PMID-sentid	Pub_year	Sent_text	comp_official_name	comp_offset	protein_name	organism	prot_offset
16689931-8	2006	In the presence of phospholipid membranes, ESAT-6, but not CFP-10 and the complex, showed an increase in alpha-helical content and enhanced thermal stability.	Phospholipids	19-31	ESAT-6 protein EsxA	Mycobacterium tuberculosis H37Rv	43-49
24056255-3	2013	We have demonstrated the application of this system to obtain N-formylated form of a mycobacterial secretory protein ESAT-6 which is an important target for T-cell recognition during Mycobacterium tuberculosis infection.	Nitrogen	62-63	ESAT-6 protein EsxA	Mycobacterium tuberculosis H37Rv	117-123
24056255-4	2013	The gene encoding the ESAT-6 of M. tuberculosis was inserted into a bacterial expression vector pET23a (+) resulting in a 6 x His-esat-6 fusion gene construction with histidine tag at its C-terminus.	Histidine	126-129	ESAT-6 protein EsxA	Mycobacterium tuberculosis H37Rv	22-28
24056255-4	2013	The gene encoding the ESAT-6 of M. tuberculosis was inserted into a bacterial expression vector pET23a (+) resulting in a 6 x His-esat-6 fusion gene construction with histidine tag at its C-terminus.	Histidine	167-176	ESAT-6 protein EsxA	Mycobacterium tuberculosis H37Rv	22-28
19257911-4	2009	esxG and esxH are part of ESAT-6 cluster 3, whose expression was already known to be induced by iron starvation.	Iron	96-100	ESAT-6 protein EsxA	Mycobacterium tuberculosis H37Rv	26-32
19257911-6	2009	In contrast to what we had observed in M. tuberculosis, we found that in M. smegmatis ESAT-6 cluster 3 responds only to iron and not to zinc.	Iron	120-124	ESAT-6 protein EsxA	Mycobacterium tuberculosis H37Rv	86-92
14990181-3	2004	After sequence analysis, and digestion by restriction endonuclease, Ag85B-ESAT6 was cloned into corresponding sites of the expression vector P(PRO) EXHT, and the recombinant plasmid was transformed into expressive strain E. coli DH5 alpha, induced with IPTG and fusion protein was purified by Ni-NTA purification system.	Isopropyl Thiogalactoside	253-257	ESAT-6 protein EsxA	Mycobacterium tuberculosis H37Rv	74-79
14990181-3	2004	After sequence analysis, and digestion by restriction endonuclease, Ag85B-ESAT6 was cloned into corresponding sites of the expression vector P(PRO) EXHT, and the recombinant plasmid was transformed into expressive strain E. coli DH5 alpha, induced with IPTG and fusion protein was purified by Ni-NTA purification system.	ni-nta	293-299	ESAT-6 protein EsxA	Mycobacterium tuberculosis H37Rv	74-79