PMID-sentid	Pub_year	Sent_text	comp_official_name	comp_offset	protein_name	organism	prot_offset
26059029-4	2015	The results obtained showed the presence of a large subpopulation of GFAP-expressing EGCs in the main plexuses and within the muscle layers, whereas only few GFAP-positive glial processes were found within the deeper layer of the mucosa, and they never reached the mucosal epithelium.	egcs	85-89	glial fibrillary acidic protein	Bos taurus	69-73
26059029-6	2015	Overall, our results provide the basis for future investigations aimed at elucidating the functional role of the GFAP-containing EGCs which is crucial for a better understanding of the physio-pathology of the bovine intestine.	egcs	129-133	glial fibrillary acidic protein	Bos taurus	113-117
2222397-8	1990	Concerning fetal stages, GFA-protein mRNA could be detected in the brain of a fetal calf with a body length of 28 cm by in situ hybridization using the 32P-labeled probe, and the mRNA was localized in the subpial area and the fornix.	Phosphorus-32	152-155	glial fibrillary acidic protein	Bos taurus	25-36
8509402-2	1993	The binding of S-100 protein to GFAP was investigated by fluorescence spectroscopy using acrylodan-S-100 protein and cross-linking experiments using the bifunctional cross-linker, disuccinimidyl suberate.	acrylodan	89-100	glial fibrillary acidic protein	Bos taurus	32-36
8509402-2	1993	The binding of S-100 protein to GFAP was investigated by fluorescence spectroscopy using acrylodan-S-100 protein and cross-linking experiments using the bifunctional cross-linker, disuccinimidyl suberate.	disuccinimidyl	180-194	glial fibrillary acidic protein	Bos taurus	32-36
1429673-10	1992	The phosphorylation site was determined as Ser-12 of porcine GFAP.	Serine	43-46	glial fibrillary acidic protein	Bos taurus	61-65
1861153-5	1991	Kinetic analysis of this response demonstrated that a marked decrease in GFAP mRNA was present at 12 h and continued to decrease through 72 h. To determine the reversibility of the TNF effect, astrocyte cultures were exposed to 200 U/ml TNF for varying periods of time and then cultured in fresh CD medium.	cd medium	296-305	glial fibrillary acidic protein	Bos taurus	73-77
1861153-7	1991	However, cultures incubated with 200 U/ml TNF for 48 h followed by incubation in CD medium without TNF for 7 days showed that GFAP mRNA levels had returned to 60% of the control values.	cd medium	81-90	glial fibrillary acidic protein	Bos taurus	126-130
1861153-9	1991	Polyacrylamide gel electrophoretic analysis of astrocyte cytoskeletal proteins demonstrated that GFAP levels were reduced after a 5-day incubation with 200 U/ml TNF whereas protein levels of vimentin and actin were not significantly changed.	polyacrylamide	0-14	glial fibrillary acidic protein	Bos taurus	97-101
3676806-1	1987	The distribution of messenger RNA for glial fibrillary acidic protein (GFA-protein) of cow and rat brains was studied by an in situ hybridization technique using a tritium-labeled cDNA probe and autoradiography.	Tritium	164-171	glial fibrillary acidic protein	Bos taurus	38-69
3196699-8	1988	Arginine and lysine at low concentrations slightly accelerate GFAP assembly, but above 100 mM both amino acids inhibit assembly.	Lysine	13-19	glial fibrillary acidic protein	Bos taurus	62-66
3196700-5	1988	After removal of unbound 45Ca2+, GFAP was observed to bind calcium.	Calcium	59-66	glial fibrillary acidic protein	Bos taurus	33-37
3196700-6	1988	Flow dialysis experiments showed that GFAP, dissolved in 2 mM Tris-HCl, pH 7.5, contained three classes of binding sites and 0.61 +/- 0.08 (SD), 1.7 +/- 0.4, and 4.6 +/- 0.2 sites per GFAP molecule with dissociation constants of 0.66 +/- 0.01 microM, 6.6 +/- 0.3 microM, and 44 +/- 1 microM, respectively.	Tromethamine	62-66	glial fibrillary acidic protein	Bos taurus	38-42
3196700-6	1988	Flow dialysis experiments showed that GFAP, dissolved in 2 mM Tris-HCl, pH 7.5, contained three classes of binding sites and 0.61 +/- 0.08 (SD), 1.7 +/- 0.4, and 4.6 +/- 0.2 sites per GFAP molecule with dissociation constants of 0.66 +/- 0.01 microM, 6.6 +/- 0.3 microM, and 44 +/- 1 microM, respectively.	Hydrochloric Acid	67-70	glial fibrillary acidic protein	Bos taurus	38-42
3196700-7	1988	After addition of 0.5 mM MgSO4 to the flow dialysis solution, the high- and low-affinity sites were not observed while the remaining sites (1.95 +/- 0.15 per GFAP molecule) had a Kd = 2.16 +/- 0.25 microM.	Magnesium Sulfate	25-30	glial fibrillary acidic protein	Bos taurus	158-162
3196700-9	1988	To locate the calcium-binding regions, GFAP peptides were examined for calcium binding by calcium-45 autoradiography.	Calcium	14-21	glial fibrillary acidic protein	Bos taurus	39-43
3196700-10	1988	The calcium-specific binding areas were localized in coil I. Computer-assisted analysis of the GFAP sequence revealed several EF-hand-like areas which could be the calcium binding sites.	Calcium	4-11	glial fibrillary acidic protein	Bos taurus	95-99
3196700-10	1988	The calcium-specific binding areas were localized in coil I. Computer-assisted analysis of the GFAP sequence revealed several EF-hand-like areas which could be the calcium binding sites.	Calcium	164-171	glial fibrillary acidic protein	Bos taurus	95-99
3676806-1	1987	The distribution of messenger RNA for glial fibrillary acidic protein (GFA-protein) of cow and rat brains was studied by an in situ hybridization technique using a tritium-labeled cDNA probe and autoradiography.	Tritium	164-171	glial fibrillary acidic protein	Bos taurus	71-82
6435677-3	1984	The results of these experiments show that the difference in molecular weight between the glial fibrillary acidic protein and desmin is due to a longer portion of the desmin amino terminal to the tryptophan.	Tryptophan	196-206	glial fibrillary acidic protein	Bos taurus	90-121
3196699-4	1988	The critical concentration for GFAP assembly was 0.08 +/- 0.04 mg/mL in 2 mM Tris-HCl, 60 mM KCl, and 1 mM CaCl2, pH 6.8.	Tris hydrochloride	77-85	glial fibrillary acidic protein	Bos taurus	31-35
3196699-4	1988	The critical concentration for GFAP assembly was 0.08 +/- 0.04 mg/mL in 2 mM Tris-HCl, 60 mM KCl, and 1 mM CaCl2, pH 6.8.	Potassium Chloride	93-96	glial fibrillary acidic protein	Bos taurus	31-35
3196699-4	1988	The critical concentration for GFAP assembly was 0.08 +/- 0.04 mg/mL in 2 mM Tris-HCl, 60 mM KCl, and 1 mM CaCl2, pH 6.8.	Calcium Chloride	107-112	glial fibrillary acidic protein	Bos taurus	31-35
3196699-5	1988	The Mr 38,000 rod domain of GFAP obtained by limited chymotryptic digestion is more soluble in 100 mM imidazole hydrochloride buffer, pH 6.8, than the intact molecule, and removal of the end pieces greatly reduces the ability of GFAP to form filaments.	imidazole	102-125	glial fibrillary acidic protein	Bos taurus	28-32
3196699-8	1988	Arginine and lysine at low concentrations slightly accelerate GFAP assembly, but above 100 mM both amino acids inhibit assembly.	Arginine	0-8	glial fibrillary acidic protein	Bos taurus	62-66
6435677-6	1984	Differences in the apparent amount of cysteine determined by these methods in the glial fibrillary acidic protein and 70 kDa proteins are discussed.	Cysteine	38-46	glial fibrillary acidic protein	Bos taurus	82-113
6435677-7	1984	Interchain disulfide bonds result in the formation of dimers in glial fibrillary acidic protein.	Disulfides	11-20	glial fibrillary acidic protein	Bos taurus	64-95
6684428-2	1983	After delipidation of the detergent-resistant, residual material with chloroform-methanol, glial fibrillary acidic protein (GFAP) was solubilized with 6 M urea.	Urea	155-159	glial fibrillary acidic protein	Bos taurus	124-128
6684428-5	1983	Employing SDS-polyacrylamide gel electrophoresis, the molecular weight of the purified GFAP was determined to be 51,000.	Sodium Dodecyl Sulfate	10-13	glial fibrillary acidic protein	Bos taurus	87-91
6684428-5	1983	Employing SDS-polyacrylamide gel electrophoresis, the molecular weight of the purified GFAP was determined to be 51,000.	polyacrylamide	14-28	glial fibrillary acidic protein	Bos taurus	87-91
6684428-7	1983	Upon incubation at 37 degrees C in the presence of 150 mM KCl, GFAP assembled into 10 nm-filaments.	Potassium Chloride	58-61	glial fibrillary acidic protein	Bos taurus	63-67