PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 2480518-6 1989 In the second approach we transfected 10T1/2 cells with a Mt-anti-sense c-H-ras construct which reduced p21 expression, slowed the growth rate and altered the morphology of 10T1/2 cells when induced with Zn. Zinc 204-206 Harvey rat sarcoma virus oncogene Mus musculus 72-79 2682459-3 1989 We have identified 5 potential GREs in the 5" upstream noncoding region of the mouse c-Ha-ras oncogene, two with the same hexanucleotide sequence and three with a similar sequence. hexanucleotide 122-136 Harvey rat sarcoma virus oncogene Mus musculus 85-93 2690912-0 1989 Reversible suppression by nalidixic acid of anchorage-independent growth of mouse cells transformed by 3-methylcholanthrene or an activated c-Ha-ras gene. Nalidixic Acid 26-40 Harvey rat sarcoma virus oncogene Mus musculus 140-148 2690912-1 1989 Effects of nalidixic acid and its derivatives were investigated on mouse cells transformed by methylcholanthrene or an activated c-Ha-ras oncogene. Nalidixic Acid 11-25 Harvey rat sarcoma virus oncogene Mus musculus 129-137 2690912-7 1989 Nalidixic acid suppressed growth in soft agar of NIH/3T3 mouse cells transformed by an activated c-Ha-ras, without affecting the amount of ras p21 proteins as detected by an immunoblotting analysis using a monoclonal antibody. Nalidixic Acid 0-14 Harvey rat sarcoma virus oncogene Mus musculus 97-105 2680143-0 1989 N-nitrosocimetidine as an initiator of murine skin tumors with associated H-ras oncogene activation. nitrosocimetidine 0-19 Harvey rat sarcoma virus oncogene Mus musculus 74-79 2680143-10 1989 Five were positive and four of these were found by selective oligonucleotide hybridization analysis to have an A to T transversion in the second position of codon 61 of the H-ras oncogene. Oligonucleotides 61-76 Harvey rat sarcoma virus oncogene Mus musculus 173-178 2682656-1 1989 An oncogenic 21-kDa (p21) protein (Harvey RAS protein with Val-12) has been covalently modified with a functional reagent that contains a photoactivatable aromatic azide group. Valine 59-62 Harvey rat sarcoma virus oncogene Mus musculus 35-45 2682656-1 1989 An oncogenic 21-kDa (p21) protein (Harvey RAS protein with Val-12) has been covalently modified with a functional reagent that contains a photoactivatable aromatic azide group. Azides 164-169 Harvey rat sarcoma virus oncogene Mus musculus 35-45 2472874-9 1989 The results of this experiment indicated that elevated levels of Ha-ras-specific RNA, in comparison with normal epidermal RNA, were present in papillomas and carcinomas from DMBA-initiated, TPA-promoted mice irrespective of the diet the mice were fed. Tetradecanoylphorbol Acetate 190-193 Harvey rat sarcoma virus oncogene Mus musculus 65-71 2551075-10 1989 The methylation state of the Ha-ras and Ki-ras oncogenes was also assessed in benzidine-induced hepatomas and adjacent nontumor tissue from B6C3F1 mice. benzidine 78-87 Harvey rat sarcoma virus oncogene Mus musculus 29-35 2549064-1 1989 Mutational replacements of specific residues in the GTP-binding pocket of the 21-kDa ras proteins (p21ras) reduce their GTPase activity. Guanosine Triphosphate 52-55 Harvey rat sarcoma virus oncogene Mus musculus 99-105 2549064-4 1989 alpha s in which leucine replaces glutamine 227 (corresponding to glutamine 61 of p21ras) constitutively activates adenylyl cyclase and reduces the kcat for GTP hydrolysis more than 100-fold. Glutamine 34-43 Harvey rat sarcoma virus oncogene Mus musculus 82-88 2549064-4 1989 alpha s in which leucine replaces glutamine 227 (corresponding to glutamine 61 of p21ras) constitutively activates adenylyl cyclase and reduces the kcat for GTP hydrolysis more than 100-fold. Guanosine Triphosphate 157-160 Harvey rat sarcoma virus oncogene Mus musculus 82-88 2549064-5 1989 There is a smaller reduction in GTPase activity in another mutant in which valine replaces glycine 49 (corresponding to glycine 12 of p21ras). Glycine 91-98 Harvey rat sarcoma virus oncogene Mus musculus 134-140 2549064-8 1989 We conclude that alpha s residues near glutamine 227 and glycine 49 participate in binding and hydrolysis of GTP, although the GTP binding regions of alpha s and p21ras are not identical. Glutamine 39-48 Harvey rat sarcoma virus oncogene Mus musculus 162-168 2549064-8 1989 We conclude that alpha s residues near glutamine 227 and glycine 49 participate in binding and hydrolysis of GTP, although the GTP binding regions of alpha s and p21ras are not identical. Guanosine Triphosphate 109-112 Harvey rat sarcoma virus oncogene Mus musculus 162-168 2549064-8 1989 We conclude that alpha s residues near glutamine 227 and glycine 49 participate in binding and hydrolysis of GTP, although the GTP binding regions of alpha s and p21ras are not identical. Guanosine Triphosphate 127-130 Harvey rat sarcoma virus oncogene Mus musculus 162-168 2549949-0 1989 Scrape-loaded p21ras down-regulates agonist-stimulated inositol phosphate production by a mechanism involving protein kinase C. The effect of scrape-loaded [Val-12]p21ras on agonist-stimulated phosphatidylinositol 4,5-bisphosphate (PIP2) turnover in Swiss-3T3 cells was studied. Inositol Phosphates 55-73 Harvey rat sarcoma virus oncogene Mus musculus 14-20 2543682-3 1989 In the presence of Mg2+, exchange of [alpha-32P]GDP with prebound ligand was very slow, but, as with p21ras, exchange was dramatically accelerated by excess EDTA. magnesium ion 19-23 Harvey rat sarcoma virus oncogene Mus musculus 101-107 2543682-3 1989 In the presence of Mg2+, exchange of [alpha-32P]GDP with prebound ligand was very slow, but, as with p21ras, exchange was dramatically accelerated by excess EDTA. Edetic Acid 157-161 Harvey rat sarcoma virus oncogene Mus musculus 101-107 2543682-8 1989 Analogous to p21ras, the binding activities of 9 of the 11 species were sensitive to the thiol reagent N-ethylmaleimide, and six peaks possessed detectable GTPase activity in the absence of extrinsic factors. Sulfhydryl Compounds 89-94 Harvey rat sarcoma virus oncogene Mus musculus 13-19 2543682-8 1989 Analogous to p21ras, the binding activities of 9 of the 11 species were sensitive to the thiol reagent N-ethylmaleimide, and six peaks possessed detectable GTPase activity in the absence of extrinsic factors. Ethylmaleimide 103-119 Harvey rat sarcoma virus oncogene Mus musculus 13-19 2517949-1 1989 Transformation of murine NIH3T3 fibroblasts with retroviral vectors carrying the mos, myc and the Ha-ras oncogene, respectively, was associated with a strong reduction of H2 antigen expression in the cell membrane. Hydrogen 171-173 Harvey rat sarcoma virus oncogene Mus musculus 98-104 2562161-0 1989 Plasmid-aided insertion of MMTV-LTR and ras DNAs to NIH 3T3 fibroblast cells makes them responsive to 2,3,7,8-TCDD causing overexpression of p21ras and down-regulation of EGF receptor. Polychlorinated Dibenzodioxins 102-114 Harvey rat sarcoma virus oncogene Mus musculus 141-147 2562161-1 1989 TCDD administered to NIH 3T3 fibroblast cells transfected with a plasmid containing MMTV-LTR and mouse ras DNAs caused an increased level of p21ras protein and down-regulation of EGF receptor. Polychlorinated Dibenzodioxins 0-4 Harvey rat sarcoma virus oncogene Mus musculus 141-147 2549949-4 1989 When protein kinase C was down-regulated by chronic TPA treatment, [Val-12]p21ras was no longer able to inhibit agonist-stimulated inositol phosphate production. Tetradecanoylphorbol Acetate 52-55 Harvey rat sarcoma virus oncogene Mus musculus 75-81 2549949-0 1989 Scrape-loaded p21ras down-regulates agonist-stimulated inositol phosphate production by a mechanism involving protein kinase C. The effect of scrape-loaded [Val-12]p21ras on agonist-stimulated phosphatidylinositol 4,5-bisphosphate (PIP2) turnover in Swiss-3T3 cells was studied. val-12 157-163 Harvey rat sarcoma virus oncogene Mus musculus 14-20 2549949-0 1989 Scrape-loaded p21ras down-regulates agonist-stimulated inositol phosphate production by a mechanism involving protein kinase C. The effect of scrape-loaded [Val-12]p21ras on agonist-stimulated phosphatidylinositol 4,5-bisphosphate (PIP2) turnover in Swiss-3T3 cells was studied. val-12 157-163 Harvey rat sarcoma virus oncogene Mus musculus 164-170 2549949-0 1989 Scrape-loaded p21ras down-regulates agonist-stimulated inositol phosphate production by a mechanism involving protein kinase C. The effect of scrape-loaded [Val-12]p21ras on agonist-stimulated phosphatidylinositol 4,5-bisphosphate (PIP2) turnover in Swiss-3T3 cells was studied. Phosphatidylinositol 4,5-Diphosphate 193-230 Harvey rat sarcoma virus oncogene Mus musculus 14-20 2549949-0 1989 Scrape-loaded p21ras down-regulates agonist-stimulated inositol phosphate production by a mechanism involving protein kinase C. The effect of scrape-loaded [Val-12]p21ras on agonist-stimulated phosphatidylinositol 4,5-bisphosphate (PIP2) turnover in Swiss-3T3 cells was studied. Phosphatidylinositol 4,5-Diphosphate 193-230 Harvey rat sarcoma virus oncogene Mus musculus 164-170 2549949-0 1989 Scrape-loaded p21ras down-regulates agonist-stimulated inositol phosphate production by a mechanism involving protein kinase C. The effect of scrape-loaded [Val-12]p21ras on agonist-stimulated phosphatidylinositol 4,5-bisphosphate (PIP2) turnover in Swiss-3T3 cells was studied. Phosphatidylinositol 4,5-Diphosphate 232-236 Harvey rat sarcoma virus oncogene Mus musculus 14-20 2647332-4 1989 Total cellular RNA was extracted from nude mouse tumor cell lines and analyzed by northern blot hybridization to a 32P-labeled, nick-translated Ha-ras probe. Phosphorus-32 115-118 Harvey rat sarcoma virus oncogene Mus musculus 144-150 2657577-1 1989 Expression of the activated Harvey-ras (H-ras) oncogene in cultured cells is associated with an elevated steady-state concentration of 1,2-diacylglycerol (DG), an intracellular second messenger capable of promoting cell division. 1,2-diacylglycerol 135-153 Harvey rat sarcoma virus oncogene Mus musculus 28-38 2657577-1 1989 Expression of the activated Harvey-ras (H-ras) oncogene in cultured cells is associated with an elevated steady-state concentration of 1,2-diacylglycerol (DG), an intracellular second messenger capable of promoting cell division. 1,2-diacylglycerol 135-153 Harvey rat sarcoma virus oncogene Mus musculus 40-45 2567173-0 1989 Activation of the cellular Harvey ras gene in mouse skin tumors initiated with urethane. Urethane 79-87 Harvey rat sarcoma virus oncogene Mus musculus 27-37 2561989-4 1989 In this regard, only cells transformed by mutated cellular and viral H-ras oncogenes (but not by the H-ras proto-oncogene) had elevated DAG levels compared to contact inhibited NIH/3T3 cells. Diglycerides 136-139 Harvey rat sarcoma virus oncogene Mus musculus 69-74 2648572-1 1989 The 21-kD proteins encoded by ras oncogenes (p21Ras) are modified covalently by a palmitate attached to a cysteine residue near the carboxyl terminus. Palmitates 82-91 Harvey rat sarcoma virus oncogene Mus musculus 45-51 2648572-1 1989 The 21-kD proteins encoded by ras oncogenes (p21Ras) are modified covalently by a palmitate attached to a cysteine residue near the carboxyl terminus. Cysteine 106-114 Harvey rat sarcoma virus oncogene Mus musculus 45-51 2493153-8 1989 The sequential overexpression of c-Ha-ras and c-erbB genes in a stage-specific manner and their cooperative interaction in the DMBA-induced in vivo oral carcinogenesis have been demonstrated. 9,10-Dimethyl-1,2-benzanthracene 127-131 Harvey rat sarcoma virus oncogene Mus musculus 33-41 2567173-7 1989 The results demonstrate a highly consistent activation of the Ha-ras oncogene by a specific point mutation, suggesting a functional role for an activated ras gene in the initiation of mouse skin tumors by urethane. Urethane 205-213 Harvey rat sarcoma virus oncogene Mus musculus 62-68 3380784-4 1988 The most noticeable change was observed in the increased activity of specific protein-tyrosine kinases and protein kinase C and an increased level of p21ras-associated binding of [3H]GTP. Tritium 180-182 Harvey rat sarcoma virus oncogene Mus musculus 150-156 2508660-2 1989 DNA isolated from tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) and each of these tumor promoters revealed the same mutation at the second nucleotide of codon 61 (CAA----CTA) in the c-Ha-ras gene, determined by the polymerase chain reaction procedure and DNA sequencing. 7,12-dimethylbenz 36-53 Harvey rat sarcoma virus oncogene Mus musculus 192-200 2508660-2 1989 DNA isolated from tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) and each of these tumor promoters revealed the same mutation at the second nucleotide of codon 61 (CAA----CTA) in the c-Ha-ras gene, determined by the polymerase chain reaction procedure and DNA sequencing. 9,10-Dimethyl-1,2-benzanthracene 68-72 Harvey rat sarcoma virus oncogene Mus musculus 192-200 2508660-4 1989 These results provide strong evidence that this mutation in the c-Ha-ras gene is due to a direct effect of DMBA rather than a selective effect of specific tumor promoters. 9,10-Dimethyl-1,2-benzanthracene 107-111 Harvey rat sarcoma virus oncogene Mus musculus 64-72 3063955-2 1988 After addition of dexamethasone, there is a rapid induction (within 1-3 h) of p21ras protein that is concomitant with a parallel induction of the c-Ha-ras specific mRNA. Dexamethasone 18-31 Harvey rat sarcoma virus oncogene Mus musculus 78-84 2842717-0 1988 c-H-ras-1 expression in 7,12-dimethyl benzanthracene-induced Balb/c mouse mammary hyperplasias and their tumors. 9,10-Dimethyl-1,2-benzanthracene 24-52 Harvey rat sarcoma virus oncogene Mus musculus 0-7 2842717-1 1988 Dimethylbenzanthracene (DMBA)-induced mouse mammary tumors characteristically contain an activated c-H-ras-1 gene with an A----T transversion in the 61st codon which is expressed as a rapidly migrating p21 c-H-ras. 9,10-Dimethyl-1,2-benzanthracene 0-22 Harvey rat sarcoma virus oncogene Mus musculus 99-106 2842717-1 1988 Dimethylbenzanthracene (DMBA)-induced mouse mammary tumors characteristically contain an activated c-H-ras-1 gene with an A----T transversion in the 61st codon which is expressed as a rapidly migrating p21 c-H-ras. 9,10-Dimethyl-1,2-benzanthracene 0-22 Harvey rat sarcoma virus oncogene Mus musculus 206-213 2842717-1 1988 Dimethylbenzanthracene (DMBA)-induced mouse mammary tumors characteristically contain an activated c-H-ras-1 gene with an A----T transversion in the 61st codon which is expressed as a rapidly migrating p21 c-H-ras. 9,10-Dimethyl-1,2-benzanthracene 24-28 Harvey rat sarcoma virus oncogene Mus musculus 99-106 2842717-1 1988 Dimethylbenzanthracene (DMBA)-induced mouse mammary tumors characteristically contain an activated c-H-ras-1 gene with an A----T transversion in the 61st codon which is expressed as a rapidly migrating p21 c-H-ras. 9,10-Dimethyl-1,2-benzanthracene 24-28 Harvey rat sarcoma virus oncogene Mus musculus 206-213 2675901-5 1989 These tissue samples were analyzed for mutations in the Ha-ras gene by in vitro amplification of DNA via the polymerase chain reaction combined with selective oligonucleotide hybridization. Oligonucleotides 159-174 Harvey rat sarcoma virus oncogene Mus musculus 56-62 2675901-7 1989 Our results demonstrate that approximately 15% of the glucose-6-phosphatase-negative lesions that occurred 24-28 wk after a single injection of diethylnitrosamine contain either C----A transversions at the first base or A----G transitions and A----T transversions at the second base of codon 61 of the Ha-ras gene. Diethylnitrosamine 144-162 Harvey rat sarcoma virus oncogene Mus musculus 302-308 3061796-3 1988 Transient expression of the c-Ha-ras oncogene, introduced into NIH 3T3 cells by the DEAE-dextran method, altered protein glycosylation within 25 h of transfection. DEAE-Dextran 84-96 Harvey rat sarcoma virus oncogene Mus musculus 28-36 3141783-1 1988 The postmicrosomal fraction of the extract from NIH 3T3 and BALB/c 3T3 cells stimulated the hydrolysis of GTP bound to H-ras gene product p21 by severalfold. Guanosine Triphosphate 106-109 Harvey rat sarcoma virus oncogene Mus musculus 119-124 3141785-6 1988 Conditions leading to the expression of the transforming Ha-ras gene but not those causing the induction of the normal Ha-ras gene yielded an increase in phosphatidylinositol turnover and a concomitant rise in inositol phosphates. Inositol Phosphates 210-229 Harvey rat sarcoma virus oncogene Mus musculus 57-63 3141785-8 1988 The data are consistent with a mechanism in which expression of the transforming Ha-ras gene leads to a release of Ca2+ from intracellular stores via elevated levels of inositol trisphosphate. inositol 1,2,3-trisphosphate 169-191 Harvey rat sarcoma virus oncogene Mus musculus 81-87 3380784-4 1988 The most noticeable change was observed in the increased activity of specific protein-tyrosine kinases and protein kinase C and an increased level of p21ras-associated binding of [3H]GTP. Guanosine Triphosphate 183-186 Harvey rat sarcoma virus oncogene Mus musculus 150-156 2827904-5 1988 Activating mutations in the H-ras genes from the DEN-induced mouse liver tumors were characterized by selective oligonucleotide hybridization and the detection of a new XbaI restriction site by Southern blot analysis. Diethylnitrosamine 49-52 Harvey rat sarcoma virus oncogene Mus musculus 28-33 3128410-1 1988 Previous results in a number of laboratories have demonstrated that epidermal papillomas and carcinomas induced by the two-stage protocol of initiation and promotion contain a point mutation in the 61st codon of the c-Ha-ras oncogene when the initiating agent used is 7,12-dimethylbenz[a]anthracene (DMBA). 7,12-dimethylbenz[a 268-287 Harvey rat sarcoma virus oncogene Mus musculus 216-224 3128410-1 1988 Previous results in a number of laboratories have demonstrated that epidermal papillomas and carcinomas induced by the two-stage protocol of initiation and promotion contain a point mutation in the 61st codon of the c-Ha-ras oncogene when the initiating agent used is 7,12-dimethylbenz[a]anthracene (DMBA). anthracene 288-298 Harvey rat sarcoma virus oncogene Mus musculus 216-224 3128410-1 1988 Previous results in a number of laboratories have demonstrated that epidermal papillomas and carcinomas induced by the two-stage protocol of initiation and promotion contain a point mutation in the 61st codon of the c-Ha-ras oncogene when the initiating agent used is 7,12-dimethylbenz[a]anthracene (DMBA). 9,10-Dimethyl-1,2-benzanthracene 300-304 Harvey rat sarcoma virus oncogene Mus musculus 216-224 2891437-1 1988 A mouse skin squamous cell carcinoma induced by topical application of the direct-acting alkylating agent beta-propiolactone contains an activated H-ras oncogene with an A----T transversion at the second nucleotide of codon 61. Propiolactone 106-124 Harvey rat sarcoma virus oncogene Mus musculus 147-152 2827904-5 1988 Activating mutations in the H-ras genes from the DEN-induced mouse liver tumors were characterized by selective oligonucleotide hybridization and the detection of a new XbaI restriction site by Southern blot analysis. Oligonucleotides 112-127 Harvey rat sarcoma virus oncogene Mus musculus 28-33 2827904-6 1988 In activated H-ras genes from the DEN-induced mouse liver tumor DNA, seven of 14 had a CG----AT transversion at the first base of the 61st codon, three of 14 had an AT----GC transition and four of 14 had the AT----TA transversion at the second base of codon 61. Diethylnitrosamine 34-37 Harvey rat sarcoma virus oncogene Mus musculus 13-18 2827904-8 1988 Taken together, the data suggest that the DEN-induced rat and mouse liver carcinogenesis may involve genetic targets other than or in addition to the H-ras gene. Diethylnitrosamine 42-45 Harvey rat sarcoma virus oncogene Mus musculus 150-155 3122209-3 1987 Transfection with the valine-12 allele of the human Harvey ras gene, under the control of its own promoter, was sufficient to prevent the induction of both muscle creatine kinase activity and the nicotinic acetylcholine receptor following mitogen withdrawal but did not inhibit withdrawal from the cell cycle. Valine 22-28 Harvey rat sarcoma virus oncogene Mus musculus 52-62 2835972-8 1988 We, therefore, conclude that cAMP mediates the inhibition of growth-related transformation-specific properties either by acting at steps subsequent to the expression of p21v-Ki-ras or on a pathway independent of p21ras function. Cyclic AMP 29-33 Harvey rat sarcoma virus oncogene Mus musculus 212-218 2824083-1 1987 Mouse skin papillomas and squamous cell carcinomas induced by initiation with 7,12-dimethylbenz[a]anthracene and promotion with phorbol esters, such as 12-O-tetradecanoyl phorbol-13-acetate, frequently contain an activated Harvey ras gene. Tetradecanoylphorbol Acetate 152-189 Harvey rat sarcoma virus oncogene Mus musculus 223-233 2824083-4 1987 In comparison with primary papillomas and carcinomas, shown to have a point mutation in codon 61 of the Harvey ras gene, resulting in a p21 product with the diagnostic alteration in SDS-PAGE, the papilloma cell lines exhibited neither the codon 61 mutation, nor p21 product with altered migration in SDS-PAGE. Sodium Dodecyl Sulfate 182-185 Harvey rat sarcoma virus oncogene Mus musculus 104-114 2824083-4 1987 In comparison with primary papillomas and carcinomas, shown to have a point mutation in codon 61 of the Harvey ras gene, resulting in a p21 product with the diagnostic alteration in SDS-PAGE, the papilloma cell lines exhibited neither the codon 61 mutation, nor p21 product with altered migration in SDS-PAGE. Sodium Dodecyl Sulfate 300-303 Harvey rat sarcoma virus oncogene Mus musculus 104-114 3151261-2 1988 Southern analysis of DNA from NIH 3T3 primary and secondary transformants established that four of the DMBA-transformed cell lines contained activated cellular Ki-ras, while the remaining cell line contained a transforming gene that is unrelated to Ki-ras, N-ras, and Ha-ras. 9,10-Dimethyl-1,2-benzanthracene 103-107 Harvey rat sarcoma virus oncogene Mus musculus 268-274 2889455-0 1987 Activation of the Ha-ras gene in C3H 10T1/2 cells transformed by exposure to N-methyl-N"-nitro-N-nitrosoguanidine. Methylnitronitrosoguanidine 77-113 Harvey rat sarcoma virus oncogene Mus musculus 18-24 3115617-0 1987 Elevated expression and point mutation of the Ha-ras proto-oncogene in mouse skin tumors promoted by benzoyl peroxide and other promoting agents. Benzoyl Peroxide 101-117 Harvey rat sarcoma virus oncogene Mus musculus 46-52 2889455-1 1987 A transfectable, presumably mutationally activated, c-Ha-ras gene was identified in a clonal population of 10T1/2 cells established from a Type II focus induced by exposure of a parental, wild-type population to N-methyl-N"-nitro-N-nitrosoguanidine (MNNG). Methylnitronitrosoguanidine 212-248 Harvey rat sarcoma virus oncogene Mus musculus 52-60 2889455-1 1987 A transfectable, presumably mutationally activated, c-Ha-ras gene was identified in a clonal population of 10T1/2 cells established from a Type II focus induced by exposure of a parental, wild-type population to N-methyl-N"-nitro-N-nitrosoguanidine (MNNG). Methylnitronitrosoguanidine 250-254 Harvey rat sarcoma virus oncogene Mus musculus 52-60 3495328-1 1987 The protease inhibitors antipain, leupeptin, alpha 1-antitrypsin, and epsilon-aminocaproic acid were found to inhibit transformation of NIH3T3 cells after transfection with an activated H-ras oncogene. leupeptin 34-43 Harvey rat sarcoma virus oncogene Mus musculus 186-191 3476850-6 1987 5 of 19 cell clones transformed by oncogenes (Ha-ras or SV40 ori-) were sensitive to the lethal effects of ACNU and showed the low MTR activities, but were not as much sensitive as a Ha-MuSV transformed cell clone, Ha821. Nimustine 107-111 Harvey rat sarcoma virus oncogene Mus musculus 46-52 3495328-1 1987 The protease inhibitors antipain, leupeptin, alpha 1-antitrypsin, and epsilon-aminocaproic acid were found to inhibit transformation of NIH3T3 cells after transfection with an activated H-ras oncogene. Aminocaproic Acid 70-95 Harvey rat sarcoma virus oncogene Mus musculus 186-191 2883654-7 1987 In NIH 3T3 cells carrying a glucocorticoid-inducible v-Ha-ras gene, a close correlation was found between the expression of p21ras and the loss of PDGF-stimulated [3H]InsP1 accumulation. Tritium 164-166 Harvey rat sarcoma virus oncogene Mus musculus 124-130 3099168-0 1986 Specific activation of the cellular Harvey-ras oncogene in dimethylbenzanthracene-induced mouse mammary tumors. 9,10-Dimethyl-1,2-benzanthracene 59-81 Harvey rat sarcoma virus oncogene Mus musculus 36-46 3030988-1 1987 1 alpha,25-Dihydroxyvitamin D3, a hormonally active form of vitamin D, induces anchorage-independent growth of BALB/3T3 A31-1-1 and NIH/3T3 cells with concomitant increase of their mRNA level of c-Ki-ras but not of c-Ha-ras or c-myc, through a receptor-mediated mechanism. alpha,25-dihydroxyvitamin d3 2-30 Harvey rat sarcoma virus oncogene Mus musculus 215-223 3030988-1 1987 1 alpha,25-Dihydroxyvitamin D3, a hormonally active form of vitamin D, induces anchorage-independent growth of BALB/3T3 A31-1-1 and NIH/3T3 cells with concomitant increase of their mRNA level of c-Ki-ras but not of c-Ha-ras or c-myc, through a receptor-mediated mechanism. Vitamin D 20-29 Harvey rat sarcoma virus oncogene Mus musculus 215-223 3540418-2 1986 All the MoAbs complexed p21ras from the ras gene-activated cell lines in Western immunoblot analysis and demonstrated a binding property of p21ras to guanine nucleotides. Guanine Nucleotides 150-169 Harvey rat sarcoma virus oncogene Mus musculus 24-30 3540418-2 1986 All the MoAbs complexed p21ras from the ras gene-activated cell lines in Western immunoblot analysis and demonstrated a binding property of p21ras to guanine nucleotides. Guanine Nucleotides 150-169 Harvey rat sarcoma virus oncogene Mus musculus 140-146 3767986-1 1986 cAMP-dependent protein kinase activity in the soluble fraction was decreased in both v-H-ras-transformed and activated-c-H-ras-transformed NIH3T3 cells as compared with that in NIH3T3 cells. Cyclic AMP 0-4 Harvey rat sarcoma virus oncogene Mus musculus 87-92 3759265-2 1986 Independent, HRAS1-selected chromosome-mediated transformants displayed distinctive cellular morphologies in monolayer culture and colony-forming abilities in low-melting-point agarose. Sepharose 177-184 Harvey rat sarcoma virus oncogene Mus musculus 13-18 3767986-1 1986 cAMP-dependent protein kinase activity in the soluble fraction was decreased in both v-H-ras-transformed and activated-c-H-ras-transformed NIH3T3 cells as compared with that in NIH3T3 cells. Cyclic AMP 0-4 Harvey rat sarcoma virus oncogene Mus musculus 121-126 3767986-2 1986 Both of the elution profile of type II cAMP-dependent protein kinase from DEAE-cellulose and the electrophoretic behavior of its regulatory subunits in the particulate fraction of H-ras-transformed cells are different from those of control NIH3T3 cells. Cyclic AMP 39-43 Harvey rat sarcoma virus oncogene Mus musculus 180-185 3092218-7 1986 Sequence homologies with p21ras and elongation factor Tu identify regions of the alpha chains that form the site for GTP binding and hydrolysis. Guanosine Triphosphate 117-120 Harvey rat sarcoma virus oncogene Mus musculus 25-31 2427243-5 1986 Significantly enhanced levels of Ha-ras RNA were observed in TPA-promoted papillomas as early as 7 weeks after initiation. Tetradecanoylphorbol Acetate 61-64 Harvey rat sarcoma virus oncogene Mus musculus 33-39 2427243-6 1986 Only trace amounts of Ha-ras RNA were present in untreated epidermis or epidermis treated with the (+) or (-) enantiomer followed by 2-12 treatments with TPA (pre-papilloma stage). Tetradecanoylphorbol Acetate 154-157 Harvey rat sarcoma virus oncogene Mus musculus 22-28 3016723-6 1986 Immunoprecipitation and NaDodSO4/PAGE analysis of p21 ras proteins in NIH 3T3 transformants derived from a majority of the hepatomas suggested that the activating mutations were localized in the 61st codon of the c-Ha-ras gene. nadodso4 24-32 Harvey rat sarcoma virus oncogene Mus musculus 50-57 3016738-0 1986 Mutagenesis of the Ha-ras oncogene in mouse skin tumors induced by polycyclic aromatic hydrocarbons. Polycyclic Aromatic Hydrocarbons 67-99 Harvey rat sarcoma virus oncogene Mus musculus 19-25 3014349-4 1986 Over 90% of tumours, including premalignant papillomas, initiated with dimethylbenzanthracene (DMBA) have a specific A----T transversion at the second nucleotide of codon 61 of the Harvey-ras (Ha-ras) gene. 9,10-Dimethyl-1,2-benzanthracene 71-93 Harvey rat sarcoma virus oncogene Mus musculus 181-191 3014349-4 1986 Over 90% of tumours, including premalignant papillomas, initiated with dimethylbenzanthracene (DMBA) have a specific A----T transversion at the second nucleotide of codon 61 of the Harvey-ras (Ha-ras) gene. 9,10-Dimethyl-1,2-benzanthracene 71-93 Harvey rat sarcoma virus oncogene Mus musculus 193-199 3014349-4 1986 Over 90% of tumours, including premalignant papillomas, initiated with dimethylbenzanthracene (DMBA) have a specific A----T transversion at the second nucleotide of codon 61 of the Harvey-ras (Ha-ras) gene. 9,10-Dimethyl-1,2-benzanthracene 95-99 Harvey rat sarcoma virus oncogene Mus musculus 181-191 3014349-4 1986 Over 90% of tumours, including premalignant papillomas, initiated with dimethylbenzanthracene (DMBA) have a specific A----T transversion at the second nucleotide of codon 61 of the Harvey-ras (Ha-ras) gene. 9,10-Dimethyl-1,2-benzanthracene 95-99 Harvey rat sarcoma virus oncogene Mus musculus 193-199 3520342-5 1986 When induced with heavy metal ions, mouse fibroblast lines containing this vector become dramatically sensitive to NK-mediated cytolysis concomitant with the expression of the cellular Harvey ras (c-Ha-ras) p21 protein and with cellular transformation. Metals 24-29 Harvey rat sarcoma virus oncogene Mus musculus 185-195 3520342-5 1986 When induced with heavy metal ions, mouse fibroblast lines containing this vector become dramatically sensitive to NK-mediated cytolysis concomitant with the expression of the cellular Harvey ras (c-Ha-ras) p21 protein and with cellular transformation. Metals 24-29 Harvey rat sarcoma virus oncogene Mus musculus 197-205 29760048-2 2018 In this study, we treat mice bearing Hras-driven poorly differentiated and anaplastic thyroid cancers (Tpo-Cre/HrasG12V/p53flox/flox ) with the FTI tipifarnib. tipifarnib 148-158 Harvey rat sarcoma virus oncogene Mus musculus 37-41 3011420-0 1986 Deletion mutants of Harvey ras p21 protein reveal the absolute requirement of at least two distant regions for GTP-binding and transforming activities. Guanosine Triphosphate 111-114 Harvey rat sarcoma virus oncogene Mus musculus 20-30 2992503-0 1985 Two classes of cAMP analogs synergistically inhibit p21 ras protein synthesis and phenotypic transformation of NIH/3T3 cells transfected with Ha-MuSV DNA. Cyclic AMP 15-19 Harvey rat sarcoma virus oncogene Mus musculus 52-59 2992503-3 1985 Treatment of cells with two classes of cAMP analogs which are selective for the two different cAMP-binding sites of type II protein kinase, in combination, synergistically inhibited both p21 ras protein synthesis and phenotypic transformation. Cyclic AMP 39-43 Harvey rat sarcoma virus oncogene Mus musculus 187-194 2992503-3 1985 Treatment of cells with two classes of cAMP analogs which are selective for the two different cAMP-binding sites of type II protein kinase, in combination, synergistically inhibited both p21 ras protein synthesis and phenotypic transformation. Cyclic AMP 94-98 Harvey rat sarcoma virus oncogene Mus musculus 187-194 32392918-6 2020 Pretreating cells with either NAC or DPI significantly enhanced the oncogenic H-Ras-mediated down-regulation of mdr1b expression and markedly prevented doxorubicin-induced cell death. Acetylcysteine 30-33 Harvey rat sarcoma virus oncogene Mus musculus 78-83 32392918-6 2020 Pretreating cells with either NAC or DPI significantly enhanced the oncogenic H-Ras-mediated down-regulation of mdr1b expression and markedly prevented doxorubicin-induced cell death. diphenyleneiodonium 37-40 Harvey rat sarcoma virus oncogene Mus musculus 78-83 31873167-6 2019 In contrast, Wdr76Li-TG mice showed increased HFD-induced obesity, insulin resistance with reduced HRas levels. Lithium 13-20 Harvey rat sarcoma virus oncogene Mus musculus 99-103 4064247-5 1985 In contrast a BPL-induced mouse skin tumor showed evidence of containing activated H-ras. Propiolactone 14-17 Harvey rat sarcoma virus oncogene Mus musculus 83-88 2866765-0 1985 Transformation of BALB/3T3 cells with EJ/T24/H-ras oncogene inhibits adenylate cyclase response to beta-adrenergic agonist while increases muscarinic receptor dependent hydrolysis of inositol lipids. inositol lipids 183-198 Harvey rat sarcoma virus oncogene Mus musculus 45-50 32970285-10 2021 MiR-338-3p directly bound to circZNF609 and HRAS, and circZNF609 knockdown repressed NPC cell malignant properties by binding to miR-338-3p. mir-338-3p 0-10 Harvey rat sarcoma virus oncogene Mus musculus 44-48 32970285-11 2021 MiR-338-3p was low in NPC, and miR-338-3p restoration performed anti-tumor effects in cells of NPC by targeting HRAS. mir-338-3p 31-41 Harvey rat sarcoma virus oncogene Mus musculus 112-116 32968439-10 2020 The results of the current study suggest that BZNQ exerts its effect by downregulating H-Ras protein expression and Ras-mediated signaling pathways. bznq 46-50 Harvey rat sarcoma virus oncogene Mus musculus 87-92 31529243-6 2020 Topical DMBA and TPA application resulted in a significant increase in the protein levels, immunoreactivity, and mRNA expression of HRAS, HIF1alpha, Akt, and PTEN (p < 0.05). 9,10-Dimethyl-1,2-benzanthracene 8-12 Harvey rat sarcoma virus oncogene Mus musculus 132-136 31529243-6 2020 Topical DMBA and TPA application resulted in a significant increase in the protein levels, immunoreactivity, and mRNA expression of HRAS, HIF1alpha, Akt, and PTEN (p < 0.05). Tetradecanoylphorbol Acetate 17-20 Harvey rat sarcoma virus oncogene Mus musculus 132-136 31529243-8 2020 CaFB application reduced the protein levels, immunoreactivity, and mRNA expressions of HRAS, HIF1alpha, Akt, and PTEN and also decreased the number of TUNEL-positive cells. calcium fructoborate 0-4 Harvey rat sarcoma virus oncogene Mus musculus 87-91 32395078-6 2020 Pretreating cells with either NAC or DPI significantly enhanced the oncogenic H-Ras-mediated down-regulation of mdr1b expression and markedly prevented doxorubicin-induced cell death. Acetylcysteine 30-33 Harvey rat sarcoma virus oncogene Mus musculus 78-83 32395078-6 2020 Pretreating cells with either NAC or DPI significantly enhanced the oncogenic H-Ras-mediated down-regulation of mdr1b expression and markedly prevented doxorubicin-induced cell death. diphenyleneiodonium 37-40 Harvey rat sarcoma virus oncogene Mus musculus 78-83 31906952-13 2020 In CD40 signaling, Ras isoforms also showed effector specificity; while H-Ras and K-Ras showed specificity for phosphatidyl inositol-3 kinase activation at high dose of CD40 stimulation, N-Ras primarily associated with Raf-1 at low dose of CD40 stimulation. Phosphatidylinositols 111-132 Harvey rat sarcoma virus oncogene Mus musculus 72-77 31061957-6 2019 Both HRAS- and HRAS/Myc-induced tumors showed decreased DNA methylation levels of Line1 and Igf2 differentially methylated region 1 and increased nuclear accumulation of 5-hydroxymethylcytosine, suggesting a state of global DNA hypomethylation. 5-hydroxymethylcytosine 170-193 Harvey rat sarcoma virus oncogene Mus musculus 5-9 31061957-6 2019 Both HRAS- and HRAS/Myc-induced tumors showed decreased DNA methylation levels of Line1 and Igf2 differentially methylated region 1 and increased nuclear accumulation of 5-hydroxymethylcytosine, suggesting a state of global DNA hypomethylation. 5-hydroxymethylcytosine 170-193 Harvey rat sarcoma virus oncogene Mus musculus 15-19 29760048-7 2018 Cell lines modified with these genetic lesions recapitulated tipifarnib resistance in vivo This study demonstrates the feasibility of targeting Ras membrane association in cancers in vivo and predicts combination therapies that confer additional benefit.Significance: Tipifarnib effectively inhibits oncogenic HRAS-driven tumorigenesis and abrogating adaptive signaling improves responses. tipifarnib 61-71 Harvey rat sarcoma virus oncogene Mus musculus 310-314 27207659-5 2016 Mammary tumors that followed DMBA exposure nearly always activated the Ras pathway via somatic Hras(CAA61CTA) mutations. 9,10-Dimethyl-1,2-benzanthracene 29-33 Harvey rat sarcoma virus oncogene Mus musculus 95-99 29054855-7 2018 Genetic modulation studies in cardiomyocytes and cardiac and embryonic fibroblasts revealed that the lack of H-Ras down-regulates the B-RAF/MEK/ERK pathway, which induces the glycogen synthase kinase-3beta-dependent activation of the transcription factor, cAMP response element-binding protein, which is responsible for PKG-Ibeta overexpression in H -ras-/- mouse embryonic fibroblasts. Cyclic AMP 256-260 Harvey rat sarcoma virus oncogene Mus musculus 109-114 28856719-6 2017 Increases in active ERK and Nitric Oxide (NO) were present in HRas mutants and inhibition of NO synthase (NOS) or MEK each partially rescued myelin decompaction. Nitric Oxide 28-40 Harvey rat sarcoma virus oncogene Mus musculus 62-66 28335476-8 2017 In a DMBA-induced skin hyperplasia assay in "Sensitivity to Carcinogenesis" (SENCAR) mice, both topical and oral SWT inhibited DMBA-induced epidermal hyperplasia, expression of the proliferation marker Proliferating cell nuclear antigen (PCNA), and H-ras mutations. 9,10-Dimethyl-1,2-benzanthracene 127-131 Harvey rat sarcoma virus oncogene Mus musculus 249-254 26921394-0 2016 High Sensitivity of an Ha-RAS Transgenic Model of Superficial Bladder Cancer to Metformin Is Associated with ~240-Fold Higher Drug Concentration in Urine than Serum. Metformin 80-89 Harvey rat sarcoma virus oncogene Mus musculus 23-29 27455813-4 2016 We showed that gestational arsenic exposure increased hepatic tumors having activated oncogene Ha-ras by C to A mutation. Arsenic 27-34 Harvey rat sarcoma virus oncogene Mus musculus 95-101 27185863-0 2016 Retinoic-acid-mediated HRas stabilization induces neuronal differentiation of neural stem cells during brain development. Tretinoin 0-13 Harvey rat sarcoma virus oncogene Mus musculus 23-27 27185863-5 2016 Retinoic acid, an active metabolite of vitamin A, promoted neuronal differentiation of NSCs by stabilizing HRas, and HRas knockdown blocked the retinoic acid effect. Tretinoin 0-13 Harvey rat sarcoma virus oncogene Mus musculus 107-111 27185863-5 2016 Retinoic acid, an active metabolite of vitamin A, promoted neuronal differentiation of NSCs by stabilizing HRas, and HRas knockdown blocked the retinoic acid effect. Vitamin A 39-48 Harvey rat sarcoma virus oncogene Mus musculus 107-111 27185863-5 2016 Retinoic acid, an active metabolite of vitamin A, promoted neuronal differentiation of NSCs by stabilizing HRas, and HRas knockdown blocked the retinoic acid effect. Tretinoin 144-157 Harvey rat sarcoma virus oncogene Mus musculus 117-121 27185863-6 2016 Vitamin-A-deficient mice displayed abnormal brain development with reduced HRas levels and a reduced thickness of the postmitotic region containing differentiated neurons. Vitamin A 0-9 Harvey rat sarcoma virus oncogene Mus musculus 75-79 27185863-7 2016 All of these abnormal phenotypes were rescued with the restoration of HRas protein levels achieved upon feeding with a retinoic-acid-supplemented diet. Tretinoin 119-132 Harvey rat sarcoma virus oncogene Mus musculus 70-74 27185863-8 2016 In summary, this study shows that retinoic acid stabilizes HRas protein during neurogenesis, and that this is required for NSC differentiation into neurons and murine brain development. Tretinoin 34-47 Harvey rat sarcoma virus oncogene Mus musculus 59-63 27382431-4 2016 Here we show that immortalized or HRAS-transformed mullerian epithelial cells contain a subpopulation of polyploid giant cells that grow as multicellular spheroids expressing hematopoietic markers in response to treatment with CoCl2. cobaltous chloride 227-232 Harvey rat sarcoma virus oncogene Mus musculus 34-38 25774517-3 2015 Expression of a constitutively active H-Ras mutant inhibited the amiloride-sensitive current. Amiloride 65-74 Harvey rat sarcoma virus oncogene Mus musculus 38-43 25885317-5 2015 Stable cell lines of human breast cancer (MCF-7-ras) and mouse NIH3T3 fibroblasts (7-4) harboring the inducible Ha-ras (val12) oncogene, which could be induced by isopropylthio-beta-D-galactoside (IPTG), were used to clarify the relationship between Ras and the up-regulated genes. Isopropyl Thiogalactoside 163-195 Harvey rat sarcoma virus oncogene Mus musculus 112-118 25885317-5 2015 Stable cell lines of human breast cancer (MCF-7-ras) and mouse NIH3T3 fibroblasts (7-4) harboring the inducible Ha-ras (val12) oncogene, which could be induced by isopropylthio-beta-D-galactoside (IPTG), were used to clarify the relationship between Ras and the up-regulated genes. Isopropyl Thiogalactoside 197-201 Harvey rat sarcoma virus oncogene Mus musculus 112-118 25774517-4 2015 The H-Ras-mediated signalling pathway that inhibits activity of ENaC involves c-Raf, and that the inhibitory effect of H-Ras on ENaC is abolished by the MEK1/2 inhibitor, PD98059. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 171-178 Harvey rat sarcoma virus oncogene Mus musculus 119-124 26168291-6 2015 We found that the vast majority of DMBA-induced SCCs presented recurrent mutations in Hras, Kras or Rras2 and mutations in several additional putative oncogenes and tumor-suppressor genes. 9,10-Dimethyl-1,2-benzanthracene 35-39 Harvey rat sarcoma virus oncogene Mus musculus 86-90 25774517-4 2015 The H-Ras-mediated signalling pathway that inhibits activity of ENaC involves c-Raf, and that the inhibitory effect of H-Ras on ENaC is abolished by the MEK1/2 inhibitor, PD98059. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 171-178 Harvey rat sarcoma virus oncogene Mus musculus 4-9 25489104-3 2014 Here we report that mice lacking DUSP5 show a greatly increased sensitivity to mutant Harvey-Ras (HRas(Q61L))-driven papilloma formation in the 7,12-Dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA) model of skin carcinogenesis. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 213-217 Harvey rat sarcoma virus oncogene Mus musculus 86-96 25694611-2 2015 Skin tumors produced by polyaromatic hydrocarbons, such as 7,12-dimethylbenz(a)anthracene (DMBA), often harbor an H-ras point mutation, suggesting that it is a poor target for early immunosurveillance. polyaromatic hydrocarbons 24-49 Harvey rat sarcoma virus oncogene Mus musculus 114-119 25694611-2 2015 Skin tumors produced by polyaromatic hydrocarbons, such as 7,12-dimethylbenz(a)anthracene (DMBA), often harbor an H-ras point mutation, suggesting that it is a poor target for early immunosurveillance. 7,12-dimethylbenz(a) 59-79 Harvey rat sarcoma virus oncogene Mus musculus 114-119 25694611-2 2015 Skin tumors produced by polyaromatic hydrocarbons, such as 7,12-dimethylbenz(a)anthracene (DMBA), often harbor an H-ras point mutation, suggesting that it is a poor target for early immunosurveillance. anthracene 79-89 Harvey rat sarcoma virus oncogene Mus musculus 114-119 25694611-2 2015 Skin tumors produced by polyaromatic hydrocarbons, such as 7,12-dimethylbenz(a)anthracene (DMBA), often harbor an H-ras point mutation, suggesting that it is a poor target for early immunosurveillance. 9,10-Dimethyl-1,2-benzanthracene 91-95 Harvey rat sarcoma virus oncogene Mus musculus 114-119 25694611-3 2015 The application of pyrosequencing and allele-specific PCR techniques established that mutations in the genome and expression of the Mut H-ras gene could be detected as early as 1 d after DMBA application. 9,10-Dimethyl-1,2-benzanthracene 187-191 Harvey rat sarcoma virus oncogene Mus musculus 136-141 25694611-6 2015 Mut H-ras, but not wild-type H-ras, epitope-focused vaccination generated specific CTLs and inhibited DMBA-induced tumor initiation, growth, and progression in preventative and therapeutic settings. 9,10-Dimethyl-1,2-benzanthracene 102-106 Harvey rat sarcoma virus oncogene Mus musculus 4-9 25789788-6 2015 Importantly, Suv39h1 overexpression in mice confers resistance to a DMBA/TPA induced skin carcinogenesis protocol that is characterized by the accumulation of activating H-ras mutations. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 68-72 Harvey rat sarcoma virus oncogene Mus musculus 170-175 25789788-6 2015 Importantly, Suv39h1 overexpression in mice confers resistance to a DMBA/TPA induced skin carcinogenesis protocol that is characterized by the accumulation of activating H-ras mutations. Tetradecanoylphorbol Acetate 73-76 Harvey rat sarcoma virus oncogene Mus musculus 170-175 25489104-3 2014 Here we report that mice lacking DUSP5 show a greatly increased sensitivity to mutant Harvey-Ras (HRas(Q61L))-driven papilloma formation in the 7,12-Dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA) model of skin carcinogenesis. 7,12-dimethylbenz 144-161 Harvey rat sarcoma virus oncogene Mus musculus 86-96 25489104-3 2014 Here we report that mice lacking DUSP5 show a greatly increased sensitivity to mutant Harvey-Ras (HRas(Q61L))-driven papilloma formation in the 7,12-Dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA) model of skin carcinogenesis. anthracene 164-174 Harvey rat sarcoma virus oncogene Mus musculus 86-96 25489104-3 2014 Here we report that mice lacking DUSP5 show a greatly increased sensitivity to mutant Harvey-Ras (HRas(Q61L))-driven papilloma formation in the 7,12-Dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA) model of skin carcinogenesis. Tetradecanoylphorbol Acetate 175-211 Harvey rat sarcoma virus oncogene Mus musculus 86-96 25489104-3 2014 Here we report that mice lacking DUSP5 show a greatly increased sensitivity to mutant Harvey-Ras (HRas(Q61L))-driven papilloma formation in the 7,12-Dimethylbenz[a]anthracene/12-O-tetradecanoylphorbol-13-acetate (DMBA/TPA) model of skin carcinogenesis. Tetradecanoylphorbol Acetate 218-221 Harvey rat sarcoma virus oncogene Mus musculus 86-96 24240680-11 2014 When tested in lines harboring HRAS, NRAS or KRAS mutations, SCH66336 delocalized, inhibited signaling and preferentially inhibited growth only of HRAS-mutant lines. lonafarnib 61-69 Harvey rat sarcoma virus oncogene Mus musculus 147-151 25239452-4 2014 In this study, we compared FDG uptake in mammary tumors driven by the Akt1, c-MYC, HER2/neu, Wnt1, or H-Ras oncogenes in genetically engineered mice, correlating it to tumor growth, cell proliferation, and expression levels of gene involved in key steps of glycolytic metabolism. Fluorodeoxyglucose F18 27-30 Harvey rat sarcoma virus oncogene Mus musculus 102-107 24240680-4 2014 Treatment of Caggs-Cre/FR-Hras(G12V) mice with TPA alone was sufficient to trigger papilloma development with a shorter latency and an ~10-fold greater tumor burden than DMBA/TPA-treated WT-controls. Tetradecanoylphorbol Acetate 47-50 Harvey rat sarcoma virus oncogene Mus musculus 26-30 24240680-5 2014 Hras(G12V) allele copy number was increased in all papillomas induced by TPA. Tetradecanoylphorbol Acetate 73-76 Harvey rat sarcoma virus oncogene Mus musculus 0-4 24240680-12 2014 Treatment with SCH66336 also induced near-complete regression of papillomas of TPA-treated Hras(G12V) knock-in mice. lonafarnib 15-23 Harvey rat sarcoma virus oncogene Mus musculus 91-95 24240680-6 2014 DMBA/TPA treatment of Hras(G12V) knock-in mice induced an even greater incidence of papillomas, which either harbored Hras(G12V) amplification or developed an Hras(Q61L) mutation in the second allele. 9,10-Dimethyl-1,2-benzanthracene 0-4 Harvey rat sarcoma virus oncogene Mus musculus 22-26 24240680-12 2014 Treatment with SCH66336 also induced near-complete regression of papillomas of TPA-treated Hras(G12V) knock-in mice. Tetradecanoylphorbol Acetate 79-82 Harvey rat sarcoma virus oncogene Mus musculus 91-95 24240680-6 2014 DMBA/TPA treatment of Hras(G12V) knock-in mice induced an even greater incidence of papillomas, which either harbored Hras(G12V) amplification or developed an Hras(Q61L) mutation in the second allele. 9,10-Dimethyl-1,2-benzanthracene 0-4 Harvey rat sarcoma virus oncogene Mus musculus 118-122 24240680-6 2014 DMBA/TPA treatment of Hras(G12V) knock-in mice induced an even greater incidence of papillomas, which either harbored Hras(G12V) amplification or developed an Hras(Q61L) mutation in the second allele. 9,10-Dimethyl-1,2-benzanthracene 0-4 Harvey rat sarcoma virus oncogene Mus musculus 118-122 24858502-6 2014 Using the same experimental model, we found that maternal arsenite exposure increases the incidence of hepatic tumors caused by a somatic mutation of the C61A Ha-ras gene, which encodes an activated oncogenic Ha-ras protein. arsenite 58-66 Harvey rat sarcoma virus oncogene Mus musculus 159-165 24240680-6 2014 DMBA/TPA treatment of Hras(G12V) knock-in mice induced an even greater incidence of papillomas, which either harbored Hras(G12V) amplification or developed an Hras(Q61L) mutation in the second allele. Tetradecanoylphorbol Acetate 5-8 Harvey rat sarcoma virus oncogene Mus musculus 22-26 24240680-6 2014 DMBA/TPA treatment of Hras(G12V) knock-in mice induced an even greater incidence of papillomas, which either harbored Hras(G12V) amplification or developed an Hras(Q61L) mutation in the second allele. Tetradecanoylphorbol Acetate 5-8 Harvey rat sarcoma virus oncogene Mus musculus 118-122 24240680-6 2014 DMBA/TPA treatment of Hras(G12V) knock-in mice induced an even greater incidence of papillomas, which either harbored Hras(G12V) amplification or developed an Hras(Q61L) mutation in the second allele. Tetradecanoylphorbol Acetate 5-8 Harvey rat sarcoma virus oncogene Mus musculus 118-122 24240680-9 2014 The FTI SCH66336 blocks HRAS farnesylation and delocalizes it from the plasma membrane. lonafarnib 8-16 Harvey rat sarcoma virus oncogene Mus musculus 24-28 24240680-11 2014 When tested in lines harboring HRAS, NRAS or KRAS mutations, SCH66336 delocalized, inhibited signaling and preferentially inhibited growth only of HRAS-mutant lines. lonafarnib 61-69 Harvey rat sarcoma virus oncogene Mus musculus 31-35 24535843-1 2014 The process of hepatocarcinogenesis in the diethylnitrosamine (DEN) initiation/phenobarbital (PB) promotion mouse model involves the selective clonal outgrowth of cells harboring oncogene mutations in Ctnnb1, while spontaneous or DEN-only-induced tumors are often Ha-ras- or B-raf-mutated. Diethylnitrosamine 43-61 Harvey rat sarcoma virus oncogene Mus musculus 264-270 24535843-1 2014 The process of hepatocarcinogenesis in the diethylnitrosamine (DEN) initiation/phenobarbital (PB) promotion mouse model involves the selective clonal outgrowth of cells harboring oncogene mutations in Ctnnb1, while spontaneous or DEN-only-induced tumors are often Ha-ras- or B-raf-mutated. Diethylnitrosamine 63-66 Harvey rat sarcoma virus oncogene Mus musculus 264-270 23994691-6 2014 This was discovered by correlation between the depurinating adducts formed in mouse skin by treatment with benzo[a]pyrene, dibenzo[a,l]pyrene or 7,12-dimethylbenz[a]anthracene and the site of mutations in the Harvey-ras oncogene in mouse skin papillomas initiated by one of these PAH. Benzo(a)pyrene 107-121 Harvey rat sarcoma virus oncogene Mus musculus 209-219 23994691-6 2014 This was discovered by correlation between the depurinating adducts formed in mouse skin by treatment with benzo[a]pyrene, dibenzo[a,l]pyrene or 7,12-dimethylbenz[a]anthracene and the site of mutations in the Harvey-ras oncogene in mouse skin papillomas initiated by one of these PAH. dibenzo(a,l)pyrene 123-141 Harvey rat sarcoma virus oncogene Mus musculus 209-219 23994691-6 2014 This was discovered by correlation between the depurinating adducts formed in mouse skin by treatment with benzo[a]pyrene, dibenzo[a,l]pyrene or 7,12-dimethylbenz[a]anthracene and the site of mutations in the Harvey-ras oncogene in mouse skin papillomas initiated by one of these PAH. 7,12-dimethylbenz[a 145-164 Harvey rat sarcoma virus oncogene Mus musculus 209-219 23994691-6 2014 This was discovered by correlation between the depurinating adducts formed in mouse skin by treatment with benzo[a]pyrene, dibenzo[a,l]pyrene or 7,12-dimethylbenz[a]anthracene and the site of mutations in the Harvey-ras oncogene in mouse skin papillomas initiated by one of these PAH. anthracene 165-175 Harvey rat sarcoma virus oncogene Mus musculus 209-219 23994691-6 2014 This was discovered by correlation between the depurinating adducts formed in mouse skin by treatment with benzo[a]pyrene, dibenzo[a,l]pyrene or 7,12-dimethylbenz[a]anthracene and the site of mutations in the Harvey-ras oncogene in mouse skin papillomas initiated by one of these PAH. Polycyclic Aromatic Hydrocarbons 280-283 Harvey rat sarcoma virus oncogene Mus musculus 209-219 24858502-6 2014 Using the same experimental model, we found that maternal arsenite exposure increases the incidence of hepatic tumors caused by a somatic mutation of the C61A Ha-ras gene, which encodes an activated oncogenic Ha-ras protein. arsenite 58-66 Harvey rat sarcoma virus oncogene Mus musculus 209-215 23512660-3 2013 Reduced exposure to TPA-induced chronic inflammation causes a dramatic reduction in classical papillomas and squamous cell carcinomas (SCCs), but the mice still develop highly invasive carcinomas with EMT properties, reduced levels of Hras and Egfr signaling, and frequent Ink4/Arf deletions. Tetradecanoylphorbol Acetate 20-23 Harvey rat sarcoma virus oncogene Mus musculus 235-239 22733434-6 2013 We focused on the tumor-related genes, p16(INK4a) , RASSF1A, Ha-ras and ER-alpha as target genes, because their expression and promoter methylation status in mice have been reported to be affected by long-term arsenic exposure. Arsenic 210-217 Harvey rat sarcoma virus oncogene Mus musculus 61-67 24694812-5 2014 This potent cardioprotective effect resulted from strong suppression of H-Ras signaling activated by electrophilic stimulation with 8-nitro-cGMP functioning as a second messenger for the redox signaling induced by NO and ROS. 8-nitroguanosine 3',5'-cyclic monophosphate 132-144 Harvey rat sarcoma virus oncogene Mus musculus 72-77 24694812-5 2014 This potent cardioprotective effect resulted from strong suppression of H-Ras signaling activated by electrophilic stimulation with 8-nitro-cGMP functioning as a second messenger for the redox signaling induced by NO and ROS. Reactive Oxygen Species 221-224 Harvey rat sarcoma virus oncogene Mus musculus 72-77 23157314-3 2013 For example, it can react with particular protein Cys thiols because of its electrophilicity and can cause unique post-translational modifications of redox-sensor proteins such as Keap1 and H-Ras. Cysteine 50-53 Harvey rat sarcoma virus oncogene Mus musculus 190-195 23157314-3 2013 For example, it can react with particular protein Cys thiols because of its electrophilicity and can cause unique post-translational modifications of redox-sensor proteins such as Keap1 and H-Ras. Sulfhydryl Compounds 54-60 Harvey rat sarcoma virus oncogene Mus musculus 190-195 24038307-0 2013 ACB-PCR measurement of spontaneous and furan-induced H-ras codon 61 CAA to CTA and CAA to AAA mutation in B6C3F1 mouse liver. furan 39-44 Harvey rat sarcoma virus oncogene Mus musculus 53-58 24038307-2 2013 H-ras codon 61 mutations have been detected in spontaneous liver tumors of B6C3F1 mice, and the fraction of liver tumors carrying H-ras codon 61 CAA to AAA mutation increased in furan-treated mice. furan 178-183 Harvey rat sarcoma virus oncogene Mus musculus 0-5 24038307-2 2013 H-ras codon 61 mutations have been detected in spontaneous liver tumors of B6C3F1 mice, and the fraction of liver tumors carrying H-ras codon 61 CAA to AAA mutation increased in furan-treated mice. furan 178-183 Harvey rat sarcoma virus oncogene Mus musculus 130-135 24038307-7 2013 Several furan-treated mice had H-ras codon 61 AAA or CTA MFs greater than those measured in control mice and lower bound estimates of induced MF were calculated. furan 8-13 Harvey rat sarcoma virus oncogene Mus musculus 31-36 23136424-0 2012 The small G protein H-Ras in the mesolimbic system is a molecular gateway to alcohol-seeking and excessive drinking behaviors. Alcohols 77-84 Harvey rat sarcoma virus oncogene Mus musculus 20-25 23136424-2 2012 Here, we report that the GTP binding protein, H-Ras in the nucleus accumbens (NAc) plays a key role in neuroadaptations that underlie excessive alcohol-drinking behaviors. Guanosine Triphosphate 25-28 Harvey rat sarcoma virus oncogene Mus musculus 46-51 23136424-2 2012 Here, we report that the GTP binding protein, H-Ras in the nucleus accumbens (NAc) plays a key role in neuroadaptations that underlie excessive alcohol-drinking behaviors. Alcohols 144-151 Harvey rat sarcoma virus oncogene Mus musculus 46-51 23136424-3 2012 Specifically, acute (15 min) systemic administration of alcohol (2.5 g/kg) leads to the activation of H-Ras in the NAc of mice, which is observed even 24 h later. Alcohols 56-63 Harvey rat sarcoma virus oncogene Mus musculus 102-107 23136424-5 2012 Using the same procedures, we provide evidence suggesting that the exchange factor GRF1 is upstream of H-Ras activation by alcohol. Alcohols 123-130 Harvey rat sarcoma virus oncogene Mus musculus 103-108 23136424-6 2012 Importantly, we show that infection of mice NAc with lentivirus expressing a short hairpin RNA that targets the H-Ras gene produces a significant reduction of voluntary consumption of 20% alcohol. Alcohols 188-195 Harvey rat sarcoma virus oncogene Mus musculus 112-117 23136424-10 2012 Together, these results position H-Ras as a central molecular mediator of alcohol"s actions within the mesolimbic system and put forward the potential value of the enzyme as a novel target to treat alcohol use disorders. Alcohols 74-81 Harvey rat sarcoma virus oncogene Mus musculus 33-38 23136424-10 2012 Together, these results position H-Ras as a central molecular mediator of alcohol"s actions within the mesolimbic system and put forward the potential value of the enzyme as a novel target to treat alcohol use disorders. Alcohols 198-205 Harvey rat sarcoma virus oncogene Mus musculus 33-38 22729866-0 2012 ACB-PCR measurement of H-ras codon 61 CAA CTA mutation provides an early indication of aristolochic acid I carcinogenic effect in tumor target tissues. aristolochic acid I 87-106 Harvey rat sarcoma virus oncogene Mus musculus 23-28 22336951-7 2012 Analysis of the hepatocellular tumors indicated that the increased incidence observed in mice administered 0.70 mmol glycidamide per kg body weight was associated with A G and A T mutations at codon 61 of H-ras. glycidamide 117-128 Harvey rat sarcoma virus oncogene Mus musculus 209-214 22399013-5 2012 Transformation by constitutively active upstream pathway elements (HRAS, RAF-1, and MEK) rendered FDC-P1 cells exquisitely prone to PD0325901-induced apoptosis. mirdametinib 132-141 Harvey rat sarcoma virus oncogene Mus musculus 67-71 22700541-0 2012 Late-onset increases in oxidative stress and other tumorigenic activities and tumors with a Ha-ras mutation in the liver of adult male C3H mice gestationally exposed to arsenic. Arsenic 169-176 Harvey rat sarcoma virus oncogene Mus musculus 92-98 22700541-2 2012 Gestational arsenic exposure has been shown to increase hepatic tumors in adult male offspring of C3H mice, which spontaneously develop hepatic tumors often harboring activating Ha-ras mutation. Arsenic 12-19 Harvey rat sarcoma virus oncogene Mus musculus 178-184 22700541-4 2012 The results of this study demonstrated that gestational arsenic exposure particularly increased hepatic tumors with a C61A Ha-ras mutation. Arsenic 56-63 Harvey rat sarcoma virus oncogene Mus musculus 123-129 22700541-10 2012 These results suggested that gestational arsenic exposure induces tumor-augmenting changes, including oxidative stress and L1 activation, in a late-onset manner, which would particularly promote tumorigenic expansion of cells with a C61A Ha-ras mutation. Arsenic 41-48 Harvey rat sarcoma virus oncogene Mus musculus 238-244 22357283-7 2012 DMBA treatment induces an activating mutation in the Harvey-ras (H-ras(61)) oncogene, and this mutation was identified in most papillomas and carcinomas although several papillomas and carcinomas in K14-LMP1 and K14-LMP1/LMP2A mice lacked the mutation. 9,10-Dimethyl-1,2-benzanthracene 0-4 Harvey rat sarcoma virus oncogene Mus musculus 53-63 21784148-7 2011 The decreased in vivo GR binding coincides with significantly decreased mRNA levels and slight reductions of protein of both H-Ras and Raf-1 in perinatally arsenic-exposed mice. Arsenic 156-163 Harvey rat sarcoma virus oncogene Mus musculus 125-130 22085642-0 2012 Oxidation of HRas cysteine thiols by metabolic stress prevents palmitoylation in vivo and contributes to endothelial cell apoptosis. Cysteine 18-26 Harvey rat sarcoma virus oncogene Mus musculus 13-17 22085642-0 2012 Oxidation of HRas cysteine thiols by metabolic stress prevents palmitoylation in vivo and contributes to endothelial cell apoptosis. Sulfhydryl Compounds 27-33 Harvey rat sarcoma virus oncogene Mus musculus 13-17 22085642-2 2012 In mice fed a high-fat, high-sucrose diet and in cultured endothelial cells (ECs) treated with high palmitate and high glucose (HPHG), there was decreased HRas palmitoylation on Cys181/184 (61+-24% decrease for cardiac tissue and 38+-7.0% in ECs). Sucrose 29-36 Harvey rat sarcoma virus oncogene Mus musculus 155-159 22085642-2 2012 In mice fed a high-fat, high-sucrose diet and in cultured endothelial cells (ECs) treated with high palmitate and high glucose (HPHG), there was decreased HRas palmitoylation on Cys181/184 (61+-24% decrease for cardiac tissue and 38+-7.0% in ECs). Palmitates 100-109 Harvey rat sarcoma virus oncogene Mus musculus 155-159 22085642-2 2012 In mice fed a high-fat, high-sucrose diet and in cultured endothelial cells (ECs) treated with high palmitate and high glucose (HPHG), there was decreased HRas palmitoylation on Cys181/184 (61+-24% decrease for cardiac tissue and 38+-7.0% in ECs). Glucose 119-126 Harvey rat sarcoma virus oncogene Mus musculus 155-159 22085642-7 2012 Furthermore, the relevance of thiol oxidation was demonstrated by overexpressing manganese superoxide dismutase, which improved HRas palmitoylation and ERK phosphorylation, while lessening apoptosis in HPHG treated ECs. Sulfhydryl Compounds 30-35 Harvey rat sarcoma virus oncogene Mus musculus 128-132 22256804-8 2012 In a mouse model of HRAS Q61L-mediated skin carcinogenesis, the vemurafenib analogue PLX4720 was not an initiator or a promoter of carcinogenesis but accelerated growth of the lesions harboring HRAS mutations, and this growth was blocked by concomitant treatment with a MEK inhibitor. PLX 4720 85-92 Harvey rat sarcoma virus oncogene Mus musculus 20-24 22256804-8 2012 In a mouse model of HRAS Q61L-mediated skin carcinogenesis, the vemurafenib analogue PLX4720 was not an initiator or a promoter of carcinogenesis but accelerated growth of the lesions harboring HRAS mutations, and this growth was blocked by concomitant treatment with a MEK inhibitor. PLX 4720 85-92 Harvey rat sarcoma virus oncogene Mus musculus 194-198 21827582-2 2011 DMBA mutates the Ha-ras gene, whereas TPA promotes the growth of transformed cells by activating cellular signaling molecules. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 0-4 Harvey rat sarcoma virus oncogene Mus musculus 17-23 21567393-3 2012 Using isolated retinal endothelial cells, the effect of regulation of H-Ras downstream signaling cascade, Raf-1, MEK, and ERK, was investigated on glucose-induced activation of MMP9. Glucose 147-154 Harvey rat sarcoma virus oncogene Mus musculus 70-75 20164394-1 2010 Neurofibromin, the product of the Nf1 gene, is a guanosine triphosphatase activating protein (GAP) for p21ras (Ras) that accelerates conversion of active Ras-GTP to inactive Ras-GDP. ras-gtp 154-161 Harvey rat sarcoma virus oncogene Mus musculus 103-109 21444916-1 2011 The small guanine nucleotide binding proteins of the Ras family, including in mammals the highly homologous H-ras, N-ras, and K-ras isoforms, are rapidly activated on ligation of the T-cell antigen receptor (TCR), but whether each isoform plays specific roles in T cells is largely unknown. Guanine Nucleotides 10-28 Harvey rat sarcoma virus oncogene Mus musculus 108-113 20707599-0 2010 Zonation of heme synthesis enzymes in mouse liver and their regulation by beta-catenin and Ha-ras. Heme 12-16 Harvey rat sarcoma virus oncogene Mus musculus 91-97 20707599-3 2010 The aim of the present study was to analyze the role of beta-catenin and Ha-ras in the regulation of heme synthesis. Heme 101-105 Harvey rat sarcoma virus oncogene Mus musculus 73-79 20707599-8 2010 By contrast, activation of Ha-ras repressed heme synthesis-related gene expression. Heme 44-48 Harvey rat sarcoma virus oncogene Mus musculus 27-33 20707599-10 2010 The reciprocal regulation of heme synthesis by beta-catenin and Ha-ras-dependent signaling supports our previous hypothesis that antagonistic action of these pathways plays a major role in the control of zonal gene expression in healthy mouse liver and aberrant expression patterns in hepatocellular tumors. Heme 29-33 Harvey rat sarcoma virus oncogene Mus musculus 64-70 20703083-6 2010 Treatment of Tyr-HRas:Cdk4(R24C/R24C) mice with the carcinogen, DMBA/TPA resulted in a further increase in the number of nevi and melanomas developed when compared with Tyr-HRas:Cdk4(+/+) mice. Tyrosine 13-16 Harvey rat sarcoma virus oncogene Mus musculus 17-21 20703083-6 2010 Treatment of Tyr-HRas:Cdk4(R24C/R24C) mice with the carcinogen, DMBA/TPA resulted in a further increase in the number of nevi and melanomas developed when compared with Tyr-HRas:Cdk4(+/+) mice. Tyrosine 13-16 Harvey rat sarcoma virus oncogene Mus musculus 173-177 20703083-6 2010 Treatment of Tyr-HRas:Cdk4(R24C/R24C) mice with the carcinogen, DMBA/TPA resulted in a further increase in the number of nevi and melanomas developed when compared with Tyr-HRas:Cdk4(+/+) mice. dmba/tpa 64-72 Harvey rat sarcoma virus oncogene Mus musculus 17-21 20703083-6 2010 Treatment of Tyr-HRas:Cdk4(R24C/R24C) mice with the carcinogen, DMBA/TPA resulted in a further increase in the number of nevi and melanomas developed when compared with Tyr-HRas:Cdk4(+/+) mice. dmba/tpa 64-72 Harvey rat sarcoma virus oncogene Mus musculus 173-177 20703083-6 2010 Treatment of Tyr-HRas:Cdk4(R24C/R24C) mice with the carcinogen, DMBA/TPA resulted in a further increase in the number of nevi and melanomas developed when compared with Tyr-HRas:Cdk4(+/+) mice. Tyrosine 169-172 Harvey rat sarcoma virus oncogene Mus musculus 17-21 20703083-7 2010 In summary, in Tyr-HRas:Cdk4(R24C/R24C) mice, we observed that activated CDK4 cooperates with the oncogenic HRAS(G12V) protein to increase the susceptibility of melanoma development in vivo. Tyrosine 15-18 Harvey rat sarcoma virus oncogene Mus musculus 19-23 20703083-7 2010 In summary, in Tyr-HRas:Cdk4(R24C/R24C) mice, we observed that activated CDK4 cooperates with the oncogenic HRAS(G12V) protein to increase the susceptibility of melanoma development in vivo. Tyrosine 15-18 Harvey rat sarcoma virus oncogene Mus musculus 108-112 20537511-0 2010 Eugenol restricts DMBA croton oil induced skin carcinogenesis in mice: downregulation of c-Myc and H-ras, and activation of p53 dependent apoptotic pathway. Eugenol 0-7 Harvey rat sarcoma virus oncogene Mus musculus 99-104 20537511-13 2010 Eugenol treatment led to the downregulation of c-Myc, H-ras and Bcl2 expression along with upregulation of P53, Bax and active Caspase-3 expression in the skin lesions. Eugenol 0-7 Harvey rat sarcoma virus oncogene Mus musculus 54-59 20537511-14 2010 CONCLUSION: Restriction of skin carcinogenesis at dysplastic stage by eugenol was due to attenuation of c-Myc, H-ras and modification of some p53 associated gene expression. Eugenol 70-77 Harvey rat sarcoma virus oncogene Mus musculus 111-116 21521744-7 2011 Despite significant cell death, SB-resistant HRASV12G keratinocytes repopulated the primary culture that had overcome HRas-induced senescence. 4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide 32-34 Harvey rat sarcoma virus oncogene Mus musculus 118-122 21501659-4 2011 The protein product of the Nf1 gene is neurofibromin, a guanosine triphosphatase-activating protein (GAP) for p21ras (Ras) that accelerates the conversion of active Ras-GTP to inactive Ras-GDP. Guanosine Triphosphate 169-172 Harvey rat sarcoma virus oncogene Mus musculus 110-116 21501659-4 2011 The protein product of the Nf1 gene is neurofibromin, a guanosine triphosphatase-activating protein (GAP) for p21ras (Ras) that accelerates the conversion of active Ras-GTP to inactive Ras-GDP. Guanosine Diphosphate 189-192 Harvey rat sarcoma virus oncogene Mus musculus 110-116 21189290-6 2011 Moreover, H-Ras formed a complex with Cdc42 on the endomembrane, and this interaction was enhanced when H-Ras was GTP bound or when cells were stimulated by growth factors. Guanosine Triphosphate 114-117 Harvey rat sarcoma virus oncogene Mus musculus 10-15 21189290-6 2011 Moreover, H-Ras formed a complex with Cdc42 on the endomembrane, and this interaction was enhanced when H-Ras was GTP bound or when cells were stimulated by growth factors. Guanosine Triphosphate 114-117 Harvey rat sarcoma virus oncogene Mus musculus 104-109 21168264-0 2011 Chronic administration of ethanol leads to an increased incidence of hepatocellular adenoma by promoting H-ras-mutated cells. Ethanol 26-33 Harvey rat sarcoma virus oncogene Mus musculus 105-110 21168264-3 2011 We show that ethanol does not cause detectable oxidative stress in the liver at any time point and acts by promoting H-ras mutated cells. Ethanol 13-20 Harvey rat sarcoma virus oncogene Mus musculus 117-122 21108125-7 2011 DMBA-mediated Ha-ras mutations in codon 61 were reduced by up to 50% with topical applications, but much higher inhibition was observed in mice treated with different combinations. 9,10-Dimethyl-1,2-benzanthracene 0-4 Harvey rat sarcoma virus oncogene Mus musculus 14-20 20679183-0 2010 Targeted genomic disruption of h-ras induces hypotension through a NO-cGMP-PKG pathway-dependent mechanism. Cyclic GMP 70-74 Harvey rat sarcoma virus oncogene Mus musculus 31-36 20308057-7 2010 Analysis of Ras isoforms showed that both H-Ras and N-Ras depended on RasGRP1 for activation by TPA, whereas activation of K-Ras could not be detected. Tetradecanoylphorbol Acetate 96-99 Harvey rat sarcoma virus oncogene Mus musculus 42-47 20228808-8 2010 Dub3 also transformed NIH-3T3 cells and cooperated with activated H-Ras to promote growth in soft agar. Agar 98-102 Harvey rat sarcoma virus oncogene Mus musculus 66-71 20088787-5 2010 Using SKBR3 cells, a HER2+ breast cancer cell line that is naturally devoid of mutations in PI3KCA, PTEN, BRAF, and ras we show that both H-ras overexpression and expression of an oncogenic ras allele (ras V12) reduce susceptibility to lapatinib in analogy to what observed with activating PI3KCA mutations and with a constitutively active form of Akt. Lapatinib 236-245 Harvey rat sarcoma virus oncogene Mus musculus 138-143 19923925-5 2010 First, mitochondrial respiration, as measured by mitochondrial oxygen consumption, was suppressed in NIH-3T3 cells transformed with H-Ras(Q61L). Oxygen 63-69 Harvey rat sarcoma virus oncogene Mus musculus 132-137 20103723-6 2010 All combinations of resveratrol with other compounds showed a synergistic effect on hyperplasia and Ha-ras mutations. Resveratrol 20-31 Harvey rat sarcoma virus oncogene Mus musculus 100-106 19923925-7 2010 Third, inhibition of glycolysis with iodoacetic acid reduced ATP levels at a much faster rate in H-Ras(Q61L) transformed cells than in the vector control cells. Iodoacetic Acid 37-52 Harvey rat sarcoma virus oncogene Mus musculus 97-102 19923925-7 2010 Third, inhibition of glycolysis with iodoacetic acid reduced ATP levels at a much faster rate in H-Ras(Q61L) transformed cells than in the vector control cells. Adenosine Triphosphate 61-64 Harvey rat sarcoma virus oncogene Mus musculus 97-102 19567395-0 2009 Early modification of c-myc, Ha-ras and p53 expressions by chemical carcinogens (DMBA, MNU). 9,10-Dimethyl-1,2-benzanthracene 81-85 Harvey rat sarcoma virus oncogene Mus musculus 29-35 19864567-0 2009 Simvastatin inhibits the activation of p21ras and prevents the loss of dopaminergic neurons in a mouse model of Parkinson"s disease. Simvastatin 0-11 Harvey rat sarcoma virus oncogene Mus musculus 39-45 19567395-0 2009 Early modification of c-myc, Ha-ras and p53 expressions by chemical carcinogens (DMBA, MNU). Methylnitrosourea 87-90 Harvey rat sarcoma virus oncogene Mus musculus 29-35 19567395-3 2009 Elevated expression of c-myc and Ha-ras genes was found in the spleen, lung, thymus and lymph nodes 6 and 12 hours after DMBA treatment and in the lung and thymus 3 hours after MNU treatment. 9,10-Dimethyl-1,2-benzanthracene 121-125 Harvey rat sarcoma virus oncogene Mus musculus 33-39 19567395-3 2009 Elevated expression of c-myc and Ha-ras genes was found in the spleen, lung, thymus and lymph nodes 6 and 12 hours after DMBA treatment and in the lung and thymus 3 hours after MNU treatment. Methylnitrosourea 177-180 Harvey rat sarcoma virus oncogene Mus musculus 33-39 18656336-0 2009 Tea polyphenols can restrict benzo[a]pyrene-induced lung carcinogenesis by altered expression of p53-associated genes and H-ras, c-myc and cyclin D1. Polyphenols 4-15 Harvey rat sarcoma virus oncogene Mus musculus 122-127 19330805-6 2009 The results show that in mouse thymic medullary-type epithelial cell line 1 (MTEC1) Dox leads to elevated levels of H-Ras, phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), cyclin E, cyclin dependent kinase 4/2 (CDK4/CDK2), E2F3, and c-myc. Doxycycline 84-87 Harvey rat sarcoma virus oncogene Mus musculus 116-121 18656336-0 2009 Tea polyphenols can restrict benzo[a]pyrene-induced lung carcinogenesis by altered expression of p53-associated genes and H-ras, c-myc and cyclin D1. benzo[a 29-36 Harvey rat sarcoma virus oncogene Mus musculus 122-127 18656336-0 2009 Tea polyphenols can restrict benzo[a]pyrene-induced lung carcinogenesis by altered expression of p53-associated genes and H-ras, c-myc and cyclin D1. pyrene 37-43 Harvey rat sarcoma virus oncogene Mus musculus 122-127 18656336-6 2009 The tea polyphenols inhibited inflammatory response in the lung lesions on the 9th week, when decreased expression of H-ras and c-myc and increased expression of bax were noted. Polyphenols 8-19 Harvey rat sarcoma virus oncogene Mus musculus 118-123 18656336-8 2009 These observations indicate that the tea polyphenols can restrict B[a]P-induced lung carcinogenesis by differential modulation of the expression of p53 and its associated genes such as bax, bcl-2, mdm2, p21 and p27, along with H-ras, c-myc and cyclin D1, at different time points. Polyphenols 41-52 Harvey rat sarcoma virus oncogene Mus musculus 227-232 19429390-5 2009 Pathway analysis of the genes regulated by 4-OOHCPA exposure revealed a novel damage response pathway in limbs comprised of basic transcription factors, Hif1a, Ndn, Hes1 and Myog, transcription activators and repressors, Egr1 and E2f1, intracellular transducers, effectors and modulators, Bmpr1b and Pea15, and oncogenes and tumor suppressors, Hras1, Abl1, Smad1, and Ttf1. 4-oohcpa 43-51 Harvey rat sarcoma virus oncogene Mus musculus 344-349 19167486-5 2009 BCR signaling to ERK1/2 and Akt requires cyclic AMP-regulated Epac, the latter acting as a guanine nucleotide exchange factor for Rap1 and H-Ras independent of protein kinase A. Cyclic AMP 41-51 Harvey rat sarcoma virus oncogene Mus musculus 139-144 19167486-7 2009 Our data indicate that cyclic AMP-dependent Epac signals to ERK1/2 and Akt upon activation of Rap1 and H-Ras, and is involved in BCR-induced growth arrest and apoptosis in WEHI-231 cells. Cyclic AMP 23-33 Harvey rat sarcoma virus oncogene Mus musculus 103-108 18598255-1 2008 The monomeric GTP-binding protein p21Ras has been repeatedly implicated in neuronal stability and plastic changes of the adult nervous system. Guanosine Triphosphate 14-17 Harvey rat sarcoma virus oncogene Mus musculus 34-40 18560355-3 2008 Herein, we examined urethane-induced lung tumorigenesis in a new mouse model developed by replacing the Kras gene with an Hras gene in the susceptible A/J-type Pas1 locus and crossing these mice with either C57BL/6J or A/J mice. Urethane 20-28 Harvey rat sarcoma virus oncogene Mus musculus 122-126 19182994-3 2009 Further studies show that both serine-727 and tyrosine-705 of Stat3 were phosphorylated while Ha-ras was overexpressed. Serine 31-37 Harvey rat sarcoma virus oncogene Mus musculus 94-100 19182994-4 2009 Interleukin-6 (IL-6)-induced phosphorylation of tyrosine-705 and serine-727, as well as DNA-binding and transcriptional activity of Stat3 were further enhanced by Ha-ras overexpression. Tyrosine 48-56 Harvey rat sarcoma virus oncogene Mus musculus 163-169 19182994-4 2009 Interleukin-6 (IL-6)-induced phosphorylation of tyrosine-705 and serine-727, as well as DNA-binding and transcriptional activity of Stat3 were further enhanced by Ha-ras overexpression. Serine 65-71 Harvey rat sarcoma virus oncogene Mus musculus 163-169 19182994-6 2009 We demonstrate that Ha-ras and Stat3 acting together synergistically induce Stat3 phosphorylation at serine-727 phosphorylation and cyclin D1 expression and further enhance transformation and tumorigenicity of the cell. Serine 101-107 Harvey rat sarcoma virus oncogene Mus musculus 20-26 19182994-7 2009 Ha-ras-induced Stat3 phosphorylation at serine-727 plays a pivotal role in transcriptional activation of cyclin D1 and suppression of cell apoptosis. Serine 40-46 Harvey rat sarcoma virus oncogene Mus musculus 0-6 19182994-8 2009 The effect of Ha-ras on Stat3 phosphorylation at serine-727 was also detected in human bladder (T24) and lung (H460) cancer cells. Serine 49-55 Harvey rat sarcoma virus oncogene Mus musculus 14-20 18410509-7 2008 The increase in Ha-Ras levels was sensitive to the protein synthesis inhibitor, cycloheximide, suggesting that serum deprivation increases translation of preformed Ha-Ras mRNA. Cycloheximide 80-93 Harvey rat sarcoma virus oncogene Mus musculus 16-22 18410509-7 2008 The increase in Ha-Ras levels was sensitive to the protein synthesis inhibitor, cycloheximide, suggesting that serum deprivation increases translation of preformed Ha-Ras mRNA. Cycloheximide 80-93 Harvey rat sarcoma virus oncogene Mus musculus 164-170 18410509-8 2008 The late decline in Ha-Ras levels observed after 60 min was prevented by the proteasome inhibitor, MG132, as well as by the selective mitogen-activated protein kinase (MAPK) inhibitor, PD98059. benzyloxycarbonylleucyl-leucyl-leucine aldehyde 99-104 Harvey rat sarcoma virus oncogene Mus musculus 20-26 18410509-8 2008 The late decline in Ha-Ras levels observed after 60 min was prevented by the proteasome inhibitor, MG132, as well as by the selective mitogen-activated protein kinase (MAPK) inhibitor, PD98059. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 185-192 Harvey rat sarcoma virus oncogene Mus musculus 20-26 17928010-3 2008 In total, 73% (102/140) of tumors induced by a single application of N-nitrosodiethylamine or 7,12-dimethylbenz[a]anthracene contained either B-raf or Ha-ras mutations and only <3% (4/140) were mutated in both genes. 7,12-dimethylbenz[a 94-113 Harvey rat sarcoma virus oncogene Mus musculus 151-157 17928010-3 2008 In total, 73% (102/140) of tumors induced by a single application of N-nitrosodiethylamine or 7,12-dimethylbenz[a]anthracene contained either B-raf or Ha-ras mutations and only <3% (4/140) were mutated in both genes. Diethylnitrosamine 69-90 Harvey rat sarcoma virus oncogene Mus musculus 151-157 17928010-3 2008 In total, 73% (102/140) of tumors induced by a single application of N-nitrosodiethylamine or 7,12-dimethylbenz[a]anthracene contained either B-raf or Ha-ras mutations and only <3% (4/140) were mutated in both genes. anthracene 114-124 Harvey rat sarcoma virus oncogene Mus musculus 151-157 18005061-1 2007 In previous studies we found that the GTPase p21 Harvey-Ras (Ha-Ras) stimulates the production of reactive oxygen species and induces apoptosis by oxidative stress; this effect was reversed by farnesyl transferase inhibitors (FTIs). Reactive Oxygen Species 98-121 Harvey rat sarcoma virus oncogene Mus musculus 61-67 18048363-6 2008 When we decreased expression of Spry2, using a Spry2-specific shRNA, the H-Ras(V12)-transformed fibroblasts could no longer form large colonies in agarose, grow in reduced levels of serum, or form tumors in athymic mice. Sepharose 147-154 Harvey rat sarcoma virus oncogene Mus musculus 73-78 18005061-6 2007 In brain tissue, NMDA increased the protein levels of Ha-Ras, FTIs caused the accumulation of non-prenylated inactive Ras in the cytosolic fraction, and significantly reduced superoxide production and necrotic volume after excitotoxicity. N-Methylaspartate 17-21 Harvey rat sarcoma virus oncogene Mus musculus 54-60 18005061-6 2007 In brain tissue, NMDA increased the protein levels of Ha-Ras, FTIs caused the accumulation of non-prenylated inactive Ras in the cytosolic fraction, and significantly reduced superoxide production and necrotic volume after excitotoxicity. Superoxides 175-185 Harvey rat sarcoma virus oncogene Mus musculus 54-60 17646168-7 2007 The protein H-Ras and activated Ras-GTP significantly decreased in TPA-induced skin tissues of DCR-fed mice but not exercised mice. Tetradecanoylphorbol Acetate 67-70 Harvey rat sarcoma virus oncogene Mus musculus 12-17 18210756-6 2007 RESULTS: Coadministration of afobazole and DMBA resulted in a decrease of DMBA-induced overexpression of Ha-ras and p53. 2-((2-morpholino)ethylthio)-5-ethoxybenzimidazole 29-38 Harvey rat sarcoma virus oncogene Mus musculus 105-111 18210756-6 2007 RESULTS: Coadministration of afobazole and DMBA resulted in a decrease of DMBA-induced overexpression of Ha-ras and p53. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 43-47 Harvey rat sarcoma virus oncogene Mus musculus 105-111 18210756-6 2007 RESULTS: Coadministration of afobazole and DMBA resulted in a decrease of DMBA-induced overexpression of Ha-ras and p53. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 74-78 Harvey rat sarcoma virus oncogene Mus musculus 105-111 17987514-0 2007 Two cases of uveal amelanotic melanoma in transgenic Tyr-HRAS+ Ink4a/Arf heterozygous mice. Tyrosine 53-56 Harvey rat sarcoma virus oncogene Mus musculus 57-61 17987514-3 2007 Reported here are two early stage intraocular amelanotic melanomas discovered in 2 Tyr-HRAS+ Ink4a/Arf heterozygous (1 normal CKDN2A allele) transgenic FVB/n mice. Tyrosine 83-86 Harvey rat sarcoma virus oncogene Mus musculus 87-91 17987514-10 2007 Consequently, Tyr-HRAS+ Ink4a/Arf heterozygotes provide practical advantages, compared to the cataract-prone CKDN2A knockout strains, for real-time ophthalomoscopic detection and monitoring of UM while developing chemotherapeutic regimens and other research to understand the biology of UM. Tyrosine 14-17 Harvey rat sarcoma virus oncogene Mus musculus 18-22 16908535-9 2006 Additionally, Kras2-mutant tumors exhibited substantially higher levels of ras-GTP, phospho-Erk1/2, and phospho-Mek1/2 compared to Hras1-mutant tumors, suggesting the involvement of the ras/mitogen-activated protein kinase (MAPK) pathway in the acquisition of oncogene independence. Radium 15-18 Harvey rat sarcoma virus oncogene Mus musculus 131-136 17515880-2 2007 We have shown that functional activation of a small molecular weight G-protein, H-Ras, is one of the signaling steps involved in glucose-induced apoptosis of retinal capillary cells. Glucose 129-136 Harvey rat sarcoma virus oncogene Mus musculus 80-85 17515880-7 2007 Inhibition of superoxide significantly attenuated glucose-induced activation of H-Ras, Raf-1 and p-p38 MAP kinase. Superoxides 14-24 Harvey rat sarcoma virus oncogene Mus musculus 80-85 17515880-7 2007 Inhibition of superoxide significantly attenuated glucose-induced activation of H-Ras, Raf-1 and p-p38 MAP kinase. Glucose 50-57 Harvey rat sarcoma virus oncogene Mus musculus 80-85 17515880-9 2007 CONCLUSIONS: Our results clearly indicate that the activation of H-Ras and its downstream signaling pathway in the retina and its vasculature could be under the control of superoxide, and H-Ras activation in diabetes can be prevented by inhibiting superoxide accumulation. Superoxides 172-182 Harvey rat sarcoma virus oncogene Mus musculus 65-70 17515880-9 2007 CONCLUSIONS: Our results clearly indicate that the activation of H-Ras and its downstream signaling pathway in the retina and its vasculature could be under the control of superoxide, and H-Ras activation in diabetes can be prevented by inhibiting superoxide accumulation. Superoxides 172-182 Harvey rat sarcoma virus oncogene Mus musculus 188-193 17515880-9 2007 CONCLUSIONS: Our results clearly indicate that the activation of H-Ras and its downstream signaling pathway in the retina and its vasculature could be under the control of superoxide, and H-Ras activation in diabetes can be prevented by inhibiting superoxide accumulation. Superoxides 248-258 Harvey rat sarcoma virus oncogene Mus musculus 65-70 17515880-9 2007 CONCLUSIONS: Our results clearly indicate that the activation of H-Ras and its downstream signaling pathway in the retina and its vasculature could be under the control of superoxide, and H-Ras activation in diabetes can be prevented by inhibiting superoxide accumulation. Superoxides 248-258 Harvey rat sarcoma virus oncogene Mus musculus 188-193 17408635-1 2007 BACKGROUND & AIMS: The present studies evaluated the role of H-ras and its implications in the RhoA/Rho kinase (ROCK) pathway in regulating basal tone in the internal anal sphincter (IAS). Adenosine Monophosphate 12-15 Harvey rat sarcoma virus oncogene Mus musculus 65-70 16908535-9 2006 Additionally, Kras2-mutant tumors exhibited substantially higher levels of ras-GTP, phospho-Erk1/2, and phospho-Mek1/2 compared to Hras1-mutant tumors, suggesting the involvement of the ras/mitogen-activated protein kinase (MAPK) pathway in the acquisition of oncogene independence. Guanosine Triphosphate 79-82 Harvey rat sarcoma virus oncogene Mus musculus 14-19 16710563-8 2006 Third, c-Ki-Ras-GTP high basal levels suppress activation of the c-H-Ras onco-protein. Guanosine Triphosphate 16-19 Harvey rat sarcoma virus oncogene Mus musculus 65-72 16448747-0 2006 Regulation of p21/ras protein expression by diallyl sulfide in DMBA induced neoplastic changes in mouse skin. allyl sulfide 44-59 Harvey rat sarcoma virus oncogene Mus musculus 14-21 16448747-0 2006 Regulation of p21/ras protein expression by diallyl sulfide in DMBA induced neoplastic changes in mouse skin. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 63-67 Harvey rat sarcoma virus oncogene Mus musculus 14-21 16448747-5 2006 Our results showed that DAS administration leads to modulation of the DMBA-induced levels of p21/ras oncoprotein as early as 24h after the DMBA application, suggesting down-regulation of the p21/ras by DAS. allyl sulfide 24-27 Harvey rat sarcoma virus oncogene Mus musculus 93-100 16448747-5 2006 Our results showed that DAS administration leads to modulation of the DMBA-induced levels of p21/ras oncoprotein as early as 24h after the DMBA application, suggesting down-regulation of the p21/ras by DAS. allyl sulfide 24-27 Harvey rat sarcoma virus oncogene Mus musculus 97-100 16448747-5 2006 Our results showed that DAS administration leads to modulation of the DMBA-induced levels of p21/ras oncoprotein as early as 24h after the DMBA application, suggesting down-regulation of the p21/ras by DAS. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 70-74 Harvey rat sarcoma virus oncogene Mus musculus 93-100 16448747-5 2006 Our results showed that DAS administration leads to modulation of the DMBA-induced levels of p21/ras oncoprotein as early as 24h after the DMBA application, suggesting down-regulation of the p21/ras by DAS. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 70-74 Harvey rat sarcoma virus oncogene Mus musculus 97-100 16448747-5 2006 Our results showed that DAS administration leads to modulation of the DMBA-induced levels of p21/ras oncoprotein as early as 24h after the DMBA application, suggesting down-regulation of the p21/ras by DAS. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 139-143 Harvey rat sarcoma virus oncogene Mus musculus 93-100 16448747-5 2006 Our results showed that DAS administration leads to modulation of the DMBA-induced levels of p21/ras oncoprotein as early as 24h after the DMBA application, suggesting down-regulation of the p21/ras by DAS. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 139-143 Harvey rat sarcoma virus oncogene Mus musculus 97-100 16448747-5 2006 Our results showed that DAS administration leads to modulation of the DMBA-induced levels of p21/ras oncoprotein as early as 24h after the DMBA application, suggesting down-regulation of the p21/ras by DAS. allyl sulfide 202-205 Harvey rat sarcoma virus oncogene Mus musculus 93-100 16448747-7 2006 DAS administration led to increase in the levels of cytosolic p21/ras and decrease in the levels of p21/ras in membrane fractions. allyl sulfide 0-3 Harvey rat sarcoma virus oncogene Mus musculus 66-69 16448747-7 2006 DAS administration led to increase in the levels of cytosolic p21/ras and decrease in the levels of p21/ras in membrane fractions. allyl sulfide 0-3 Harvey rat sarcoma virus oncogene Mus musculus 104-107 16448747-8 2006 DAS administration was also found to down regulate the DMBA-induced H-ras mRNA level in mouse skin tumors. allyl sulfide 0-3 Harvey rat sarcoma virus oncogene Mus musculus 68-73 16448747-8 2006 DAS administration was also found to down regulate the DMBA-induced H-ras mRNA level in mouse skin tumors. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 55-59 Harvey rat sarcoma virus oncogene Mus musculus 68-73 16805855-2 2006 Previous studies showed that the mutation of c-Ha-ras induced by DMBA in the tumors of rasH2 were detected only in transgenes. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 65-69 Harvey rat sarcoma virus oncogene Mus musculus 45-53 16805855-3 2006 To examine if the difference between the codons of the c-Ha-ras gene in human and mouse contributed to the tissue-specific sensitivity to DMBA, we generated a line of transgenic mice, mras, carrying mouse c-Ha-ras genome with its own promoter. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 138-142 Harvey rat sarcoma virus oncogene Mus musculus 55-63 16805855-12 2006 These results indicate that the differences in the codon of the c-Ha-ras gene between mouse and human might contribute to the tissue-specific sensitivity of DMBA. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 157-161 Harvey rat sarcoma virus oncogene Mus musculus 64-72 16710563-9 2006 But, Y1 cells, expressing dominant negative mutant RasN17, display a rapid and transient up-regulation of c-H-Ras-GTP upon FGF2 treatment. Guanosine Triphosphate 114-117 Harvey rat sarcoma virus oncogene Mus musculus 106-113 16612794-1 2006 To elucidate an understanding into H-Ras protein network, we have established various oncogene H-Ras-expressing NIH/3T3 mouse embryonic fibroblast cell clones, which are expressing G12V H-Ras, G12R H-Ras, and G12V/T35S H-Ras proteins under the tight control of expression by an antibiotic doxycycline. Doxycycline 289-300 Harvey rat sarcoma virus oncogene Mus musculus 95-100 16242868-4 2006 In the cell proliferation assay, TCE and punicalagin suppressed the proliferation of H-ras-transformed NIH3T3 cells with a dose-dependent manner but only partially affected non-transformed NIH3T3 cells proliferation. Trichloroethylene 33-36 Harvey rat sarcoma virus oncogene Mus musculus 85-90 16242868-4 2006 In the cell proliferation assay, TCE and punicalagin suppressed the proliferation of H-ras-transformed NIH3T3 cells with a dose-dependent manner but only partially affected non-transformed NIH3T3 cells proliferation. punicalagin 41-52 Harvey rat sarcoma virus oncogene Mus musculus 85-90 16242868-5 2006 The differential cytotoxicity of TCE/punicalagin on the H-ras-transformed and non-transformed NIH3T3 cells indicated the selectivity of TCE/punicalagin against H-ras induced transformation. Trichloroethylene 33-36 Harvey rat sarcoma virus oncogene Mus musculus 56-61 16242868-5 2006 The differential cytotoxicity of TCE/punicalagin on the H-ras-transformed and non-transformed NIH3T3 cells indicated the selectivity of TCE/punicalagin against H-ras induced transformation. Trichloroethylene 33-36 Harvey rat sarcoma virus oncogene Mus musculus 160-165 16242868-5 2006 The differential cytotoxicity of TCE/punicalagin on the H-ras-transformed and non-transformed NIH3T3 cells indicated the selectivity of TCE/punicalagin against H-ras induced transformation. Trichloroethylene 136-139 Harvey rat sarcoma virus oncogene Mus musculus 56-61 16242868-5 2006 The differential cytotoxicity of TCE/punicalagin on the H-ras-transformed and non-transformed NIH3T3 cells indicated the selectivity of TCE/punicalagin against H-ras induced transformation. Trichloroethylene 136-139 Harvey rat sarcoma virus oncogene Mus musculus 160-165 16612794-1 2006 To elucidate an understanding into H-Ras protein network, we have established various oncogene H-Ras-expressing NIH/3T3 mouse embryonic fibroblast cell clones, which are expressing G12V H-Ras, G12R H-Ras, and G12V/T35S H-Ras proteins under the tight control of expression by an antibiotic doxycycline. Doxycycline 289-300 Harvey rat sarcoma virus oncogene Mus musculus 95-100 16612794-1 2006 To elucidate an understanding into H-Ras protein network, we have established various oncogene H-Ras-expressing NIH/3T3 mouse embryonic fibroblast cell clones, which are expressing G12V H-Ras, G12R H-Ras, and G12V/T35S H-Ras proteins under the tight control of expression by an antibiotic doxycycline. Doxycycline 289-300 Harvey rat sarcoma virus oncogene Mus musculus 95-100 16612794-1 2006 To elucidate an understanding into H-Ras protein network, we have established various oncogene H-Ras-expressing NIH/3T3 mouse embryonic fibroblast cell clones, which are expressing G12V H-Ras, G12R H-Ras, and G12V/T35S H-Ras proteins under the tight control of expression by an antibiotic doxycycline. Doxycycline 289-300 Harvey rat sarcoma virus oncogene Mus musculus 95-100 16274703-2 2006 Ha-ras overexpression was induced by isopropyl beta-D-thiogalactoside (IPTG). Isopropyl Thiogalactoside 37-69 Harvey rat sarcoma virus oncogene Mus musculus 0-6 16274703-2 2006 Ha-ras overexpression was induced by isopropyl beta-D-thiogalactoside (IPTG). Isopropyl Thiogalactoside 71-75 Harvey rat sarcoma virus oncogene Mus musculus 0-6 16306046-6 2006 H-Ras-specific inhibition of mechanorepression of RANKL was also demonstrated in a murine pre-osteoblast cell line (CIMC-4). cimc-4 116-122 Harvey rat sarcoma virus oncogene Mus musculus 0-5 16306046-7 2006 The requirement of cholesterol for H-Ras activation was probed; cholesterol depletion of rafts using methyl-betacyclodextrin prevented mechanical H-Ras activation. Cholesterol 64-75 Harvey rat sarcoma virus oncogene Mus musculus 146-151 16306046-7 2006 The requirement of cholesterol for H-Ras activation was probed; cholesterol depletion of rafts using methyl-betacyclodextrin prevented mechanical H-Ras activation. Cholesterol 19-30 Harvey rat sarcoma virus oncogene Mus musculus 146-151 16306046-7 2006 The requirement of cholesterol for H-Ras activation was probed; cholesterol depletion of rafts using methyl-betacyclodextrin prevented mechanical H-Ras activation. methyl-beta-cyclodextrin 101-124 Harvey rat sarcoma virus oncogene Mus musculus 146-151 16433043-0 2006 Effect of the chalcone analog E,E-bis(2-hydroxybenzylidene) acetone on the 7,12-dimethylbenz[a]anthracene-induced Ha-ras gene activity in vivo. Chalcone 14-22 Harvey rat sarcoma virus oncogene Mus musculus 114-120 16306046-8 2006 That the mechanical repression of RANKL requires activation of H-Ras, a specific isoform of Ras-GTP that is known to reside in the lipid raft microdomain, suggests that spatial arrangements are critical for generation of specific downstream events in response to mechanical signals. Guanosine Triphosphate 96-99 Harvey rat sarcoma virus oncogene Mus musculus 63-68 16433043-2 2006 In order to determine whether this promising chemopreventive activity would extend to anticarcinogenic properties, the effect of HBA on the 7,12-dimethylbenz[a]anthracene (DMBA)-induced expression of the Ha-ras gene in isolated RNA from liver, lung, kidney, spleen, thymus, lymph nodes and bone marrow of CBA/Ca inbred mice was investigated. anthracene 160-170 Harvey rat sarcoma virus oncogene Mus musculus 204-210 16433043-2 2006 In order to determine whether this promising chemopreventive activity would extend to anticarcinogenic properties, the effect of HBA on the 7,12-dimethylbenz[a]anthracene (DMBA)-induced expression of the Ha-ras gene in isolated RNA from liver, lung, kidney, spleen, thymus, lymph nodes and bone marrow of CBA/Ca inbred mice was investigated. 9,10-Dimethyl-1,2-benzanthracene 172-176 Harvey rat sarcoma virus oncogene Mus musculus 204-210 16433043-4 2006 According to the previous results, elevated Ha-ras expression has been noted even 24 h after DMBA treatment. 9,10-Dimethyl-1,2-benzanthracene 93-97 Harvey rat sarcoma virus oncogene Mus musculus 44-50 16433043-5 2006 Administration of HBA simultaneously with DMBA resulted in a decrease of the DMBA-induced Ha-ras gene expression in all the investigated tissues. E,E-bis(2-hydroxybenzylidene)acetone 18-21 Harvey rat sarcoma virus oncogene Mus musculus 90-96 16433043-5 2006 Administration of HBA simultaneously with DMBA resulted in a decrease of the DMBA-induced Ha-ras gene expression in all the investigated tissues. 9,10-Dimethyl-1,2-benzanthracene 42-46 Harvey rat sarcoma virus oncogene Mus musculus 90-96 16433043-5 2006 Administration of HBA simultaneously with DMBA resulted in a decrease of the DMBA-induced Ha-ras gene expression in all the investigated tissues. 9,10-Dimethyl-1,2-benzanthracene 77-81 Harvey rat sarcoma virus oncogene Mus musculus 90-96 16433043-7 2006 Administration of HBA 24 h prior to the DMBA treatment reduced the Ha-ras gene expression in all the tissues but the liver, where a slight elevation could be detected. 9,10-Dimethyl-1,2-benzanthracene 40-44 Harvey rat sarcoma virus oncogene Mus musculus 67-73 16433043-0 2006 Effect of the chalcone analog E,E-bis(2-hydroxybenzylidene) acetone on the 7,12-dimethylbenz[a]anthracene-induced Ha-ras gene activity in vivo. E,E-bis(2-hydroxybenzylidene)acetone 30-67 Harvey rat sarcoma virus oncogene Mus musculus 114-120 16433043-0 2006 Effect of the chalcone analog E,E-bis(2-hydroxybenzylidene) acetone on the 7,12-dimethylbenz[a]anthracene-induced Ha-ras gene activity in vivo. 7,12-dimethylbenz[a 75-94 Harvey rat sarcoma virus oncogene Mus musculus 114-120 16433043-0 2006 Effect of the chalcone analog E,E-bis(2-hydroxybenzylidene) acetone on the 7,12-dimethylbenz[a]anthracene-induced Ha-ras gene activity in vivo. anthracene 95-105 Harvey rat sarcoma virus oncogene Mus musculus 114-120 16256070-5 2005 Furthermore, tv-a-mediated delivery of various oncogenes (v-src, H-ras, myc or akt) leads to malignant transformation. 11-octadecenoic acid 13-17 Harvey rat sarcoma virus oncogene Mus musculus 65-70 17162533-0 2006 Frequent p53 and H-ras mutations in benzene- and ethylene oxide-induced mammary gland carcinomas from B6C3F1 mice. Benzene 36-43 Harvey rat sarcoma virus oncogene Mus musculus 17-22 17162533-0 2006 Frequent p53 and H-ras mutations in benzene- and ethylene oxide-induced mammary gland carcinomas from B6C3F1 mice. Ethylene Oxide 49-63 Harvey rat sarcoma virus oncogene Mus musculus 17-22 17162533-7 2006 H-ras mutations were identified in 26% (5/19) of spontaneous, 50% (7/14) of benzene-, and 33% (4/12) of ethylene oxide-induced carcinomas. Benzene 76-83 Harvey rat sarcoma virus oncogene Mus musculus 0-5 17162533-7 2006 H-ras mutations were identified in 26% (5/19) of spontaneous, 50% (7/14) of benzene-, and 33% (4/12) of ethylene oxide-induced carcinomas. Ethylene Oxide 104-118 Harvey rat sarcoma virus oncogene Mus musculus 0-5 17162533-8 2006 When H-ras mutations were present, concurrent p53 mutations were identified in 40% (2/5) of spontaneous, 71% (5/7) of benzene-, and 75% (3/4) of ethylene oxide-induced carcinomas. Benzene 118-125 Harvey rat sarcoma virus oncogene Mus musculus 5-10 17162533-8 2006 When H-ras mutations were present, concurrent p53 mutations were identified in 40% (2/5) of spontaneous, 71% (5/7) of benzene-, and 75% (3/4) of ethylene oxide-induced carcinomas. Ethylene Oxide 145-159 Harvey rat sarcoma virus oncogene Mus musculus 5-10 16288745-0 2005 Ha-Ras sensitizes transformed mouse skin cells to Anisomycin-induced apoptosis. Anisomycin 50-60 Harvey rat sarcoma virus oncogene Mus musculus 0-6 16339563-0 2005 Pure lipopolysaccharide or synthetic lipid A induces activation of p21Ras in primary macrophages through a pathway dependent on Src family kinases and PI3K. Lipid A 37-44 Harvey rat sarcoma virus oncogene Mus musculus 67-73 16339563-4 2005 We report that p21Ras is rapidly and transiently activated in murine primary macrophages stimulated with an ultra-pure preparation of LPS or with synthetic lipid A. Lipid A 156-163 Harvey rat sarcoma virus oncogene Mus musculus 15-21 16339563-6 2005 LPS-induced activation of p21Ras was inhibited in the presence of PP2, LY294002, or wortmannin, suggesting that it depends on the activity of one or more members of the Src kinase family and the subsequent activation of PI3K. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 71-79 Harvey rat sarcoma virus oncogene Mus musculus 26-32 16339563-6 2005 LPS-induced activation of p21Ras was inhibited in the presence of PP2, LY294002, or wortmannin, suggesting that it depends on the activity of one or more members of the Src kinase family and the subsequent activation of PI3K. Wortmannin 84-94 Harvey rat sarcoma virus oncogene Mus musculus 26-32 16288745-2 2005 Here, we demonstrated that a mutated Ha-Ras activity is required in Anisomycin-induced apoptosis in transformed keratinocytes. Anisomycin 68-78 Harvey rat sarcoma virus oncogene Mus musculus 37-43 16288745-3 2005 Anisomycin stimulates JNK activity and apoptosis in oncogenic Ha-Ras positive cells, but not in normal keratinocytes. Anisomycin 0-10 Harvey rat sarcoma virus oncogene Mus musculus 62-68 16288745-4 2005 This effect was demonstrated in stably transfected cells with dominant negative Ha-Ras, that protected transformed cells, and oncogenic Ha-Ras that sensitized non-transformed cells to Anisomycin-induced apoptosis. Anisomycin 184-194 Harvey rat sarcoma virus oncogene Mus musculus 136-142 16288745-6 2005 These data suggests that the oncogenic Ha-Ras is important for Anisomycin-induced JNK activation and apoptosis in transformed keratinocytes. Anisomycin 63-73 Harvey rat sarcoma virus oncogene Mus musculus 39-45 16251872-3 2005 The haeme substrate 5-aminolevulinic acid (5-ALA) (200 mg kg(-1)) was administered to mouse strains that develop mammary tumours of various histological subtypes upon expression of the transgenic oncogenes HRAS, Polyoma Virus middle T antigen, or Simian Virus 40 large T antigen in the mammary gland. 5-amino levulinic acid 20-41 Harvey rat sarcoma virus oncogene Mus musculus 206-210 16251872-3 2005 The haeme substrate 5-aminolevulinic acid (5-ALA) (200 mg kg(-1)) was administered to mouse strains that develop mammary tumours of various histological subtypes upon expression of the transgenic oncogenes HRAS, Polyoma Virus middle T antigen, or Simian Virus 40 large T antigen in the mammary gland. 5-amino levulinic acid 43-48 Harvey rat sarcoma virus oncogene Mus musculus 206-210 16271875-4 2005 Here, we identify lovastatin as a potent inhibitor of p21Ras/Mitogen Activated Protein Kinase (MAPK) activity in the brain. Lovastatin 18-28 Harvey rat sarcoma virus oncogene Mus musculus 54-93 16271875-6 2005 We report that lovastatin decreased the enhanced brain p21Ras-MAPK activity of the nf1+/- mice, rescued their LTP deficits, and reversed their spatial learning and attention impairments. Lovastatin 15-25 Harvey rat sarcoma virus oncogene Mus musculus 55-61 16117800-0 2005 Harvey-ras gene expression and epidermal cell proliferation in dibenzo[a,l]pyrene-treated early preneoplastic SENCAR mouse skin. dibenzo(a,l)pyrene 63-81 Harvey rat sarcoma virus oncogene Mus musculus 0-10 16117800-3 2005 We have investigated DB[a,l]P-treated mouse skin (12 h-7 d) for further evidence of H-ras expression and epidermal cell proliferation. dibenzo(a,l)pyrene 21-29 Harvey rat sarcoma virus oncogene Mus musculus 84-89 16117800-5 2005 DB[a,l]P-induced early preneoplastic cell proliferation correlated with H-ras and specific G1 cyclin expression. 1,2,5,6-dibenzanthracene 0-5 Harvey rat sarcoma virus oncogene Mus musculus 72-77 16117800-5 2005 DB[a,l]P-induced early preneoplastic cell proliferation correlated with H-ras and specific G1 cyclin expression. Phosphorus 6-8 Harvey rat sarcoma virus oncogene Mus musculus 72-77 16117800-6 2005 Total H-ras protein and cyclin D1 were found to increase during DB[a,l]P-induced hyperplasia, but the levels of guanosine triphosphate-bound (active) H-ras protein and cyclin E were increased during the putative clonal proliferation of codon 61-mutated cells. Guanosine Triphosphate 112-134 Harvey rat sarcoma virus oncogene Mus musculus 150-155 15800929-0 2005 Combined subcarcinogenic benzo[a]pyrene and UVA synergistically caused high tumor incidence and mutations in H-ras gene, but not p53, in SKH-1 hairless mouse skin. Benzo(a)pyrene 25-39 Harvey rat sarcoma virus oncogene Mus musculus 109-114 15800929-1 2005 Combined subcarcinogenic doses of benzo[a]pyrene (BaP) and UVA induced H-ras, but not p53, gene mutations 8 weeks before tumor emergence in SKH-1 mice. Benzo(a)pyrene 34-48 Harvey rat sarcoma virus oncogene Mus musculus 71-76 15650250-2 2004 Depurinating DNA adducts of polycyclic aromatic hydrocarbons play a major role in the initiation of cancer, as shown by the correlation between depurinating adducts and oncogenic mutations of the H-ras oncogene in mouse skin. Polycyclic Aromatic Hydrocarbons 28-60 Harvey rat sarcoma virus oncogene Mus musculus 196-201 16024603-9 2005 In K14-Pdcd4 primary keratinocytes expressing activated Ha-Ras to mimic DMBA-initiated epidermis, ODC and CDK4 protein levels were decreased by 40% and 46%, respectively. 9,10-Dimethyl-1,2-benzanthracene 72-76 Harvey rat sarcoma virus oncogene Mus musculus 56-62 15861430-0 2005 On the in vitro and in vivo properties of four locked nucleic acid nucleotides incorporated into an anti-H-Ras antisense oligonucleotide. nucleic acid nucleotides 54-78 Harvey rat sarcoma virus oncogene Mus musculus 105-110 15861430-0 2005 On the in vitro and in vivo properties of four locked nucleic acid nucleotides incorporated into an anti-H-Ras antisense oligonucleotide. Oligonucleotides 121-136 Harvey rat sarcoma virus oncogene Mus musculus 105-110 15592514-4 2005 We now screened 82 N-nitrosodiethylamine-induced liver tumors from C3H/He mice for mutations within the hotspot positions in the Ha-ras and B-raf genes. Diethylnitrosamine 19-40 Harvey rat sarcoma virus oncogene Mus musculus 129-135 16050805-7 2005 We serially examined the histopathological changes in the lung of mice administered benzo(a)pyrene and correlated them with the frequency of proliferative and apoptotic cells in situ as well as with the expression of H-ras, c-Myc, p53, and Bcl-2 genes, which play key roles in the histopathogenesis of neoplasia. Benzo(a)pyrene 84-98 Harvey rat sarcoma virus oncogene Mus musculus 217-222 15345372-1 2004 Previous research demonstrated that 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment increased the number of skin papillomas in v-Ha-ras transgenic (Tg.AC) mice that had received sodium arsenite [(As(III)] in drinking water, indicating that this model is useful for studying the toxic effects of arsenic in vivo. Tetradecanoylphorbol Acetate 36-72 Harvey rat sarcoma virus oncogene Mus musculus 134-140 15273725-6 2004 In order to address this question, Ha-Ras(G12V) was expressed ectopically in primary as well as hTERT-immortalized human esophageal keratinocytes stably expressing dominant-negative p53 mutants. htert 96-101 Harvey rat sarcoma virus oncogene Mus musculus 35-46 15355339-2 2004 In particular, transfection of codon 12 point mutated H-Ras increases CMP-Neu5Ac: Galbeta1,4GlcNAc alpha2,6-sialyltransferase I (ST6Gal I) activity in rodent fibroblasts. N-Acetyl-D-lactosamine 82-98 Harvey rat sarcoma virus oncogene Mus musculus 54-59 15378015-1 2004 Induced transformation of mouse fibroblasts was carried out by releasing tetracycline-repressed expression of an oncogenic mutant of STAT3, STAT3-C, or of v-Src or Ha-Ras. Tetracycline 73-85 Harvey rat sarcoma virus oncogene Mus musculus 164-170 15343391-6 2004 Abrogation of Stat3 function using a decoy oligonucleotide inhibited the growth of initiated keratinocytes possessing an activated Ha-ras gene, both in vitro and in vivo. Oligonucleotides 43-58 Harvey rat sarcoma virus oncogene Mus musculus 131-137 15345372-1 2004 Previous research demonstrated that 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment increased the number of skin papillomas in v-Ha-ras transgenic (Tg.AC) mice that had received sodium arsenite [(As(III)] in drinking water, indicating that this model is useful for studying the toxic effects of arsenic in vivo. Tetradecanoylphorbol Acetate 74-77 Harvey rat sarcoma virus oncogene Mus musculus 134-140 15172983-2 2004 We have previously reported that cell transformations induced by ornithine decarboxylase (ODC) and c-Ha-ras oncogene, commonly activated in various cancer cells, are associated with constitutively increased phosphorylation of c-Jun on Ser residues 63 and 73. Serine 235-238 Harvey rat sarcoma virus oncogene Mus musculus 99-107 15289454-2 2004 H-ras mutation is normally a hallmark of DMBA-TPA-induced skin tumors, but 70% of carcinomas from Pten+/- mice do not exhibit this mutation, and in all cases have lost the wild-type Pten allele. dmba-tpa 41-49 Harvey rat sarcoma virus oncogene Mus musculus 0-5 15123632-3 2004 FAK phosphorylation at tyrosine 861 was increased in both constitutively H-Ras-transformed and H-Ras-inducible NIH3T3 cells, in parallel with cell transformation. Tyrosine 23-31 Harvey rat sarcoma virus oncogene Mus musculus 73-78 15123632-3 2004 FAK phosphorylation at tyrosine 861 was increased in both constitutively H-Ras-transformed and H-Ras-inducible NIH3T3 cells, in parallel with cell transformation. Tyrosine 23-31 Harvey rat sarcoma virus oncogene Mus musculus 95-100 15123632-7 2004 Taken together, these results strongly suggest that FAK phosphorylation at tyrosine 861 is crucial for H-Ras-induced transformation through regulation of the association of FAK with p130(CAS). Tyrosine 75-83 Harvey rat sarcoma virus oncogene Mus musculus 103-108 14633658-7 2004 TBPB and TBIBHP were selected for further examination of their ability to produce mutations in codons 12, 13 and 61 of the c-Ha-ras protooncogene, i.e. those mutations known to be involved in the initiation of mouse skin tumors, because they were the only peroxides to exhibit significant positive results in all assays except the Ha-ras mutation following 4 weeks of exposure. tert-butyl peroxybenzoate 0-4 Harvey rat sarcoma virus oncogene Mus musculus 123-131 14633658-7 2004 TBPB and TBIBHP were selected for further examination of their ability to produce mutations in codons 12, 13 and 61 of the c-Ha-ras protooncogene, i.e. those mutations known to be involved in the initiation of mouse skin tumors, because they were the only peroxides to exhibit significant positive results in all assays except the Ha-ras mutation following 4 weeks of exposure. tbibhp 9-15 Harvey rat sarcoma virus oncogene Mus musculus 123-131 14633658-7 2004 TBPB and TBIBHP were selected for further examination of their ability to produce mutations in codons 12, 13 and 61 of the c-Ha-ras protooncogene, i.e. those mutations known to be involved in the initiation of mouse skin tumors, because they were the only peroxides to exhibit significant positive results in all assays except the Ha-ras mutation following 4 weeks of exposure. Peroxides 256-265 Harvey rat sarcoma virus oncogene Mus musculus 123-131 14603530-9 2004 The PKC inhibitor 1-O-hexadecyl-2-O-methyl-rac-glycerol (HMG), significantly reduced p21Ras-mediated tumor growth in vivo in a nude mouse MIA-PaCa-2 xenograft model. 1-O-hexadecyl-2-O-methylglycerol 18-55 Harvey rat sarcoma virus oncogene Mus musculus 85-91 14603530-9 2004 The PKC inhibitor 1-O-hexadecyl-2-O-methyl-rac-glycerol (HMG), significantly reduced p21Ras-mediated tumor growth in vivo in a nude mouse MIA-PaCa-2 xenograft model. 1-O-hexadecyl-2-O-methylglycerol 57-60 Harvey rat sarcoma virus oncogene Mus musculus 85-91 14695209-0 2003 Geranylgeranyltransferase I inhibitor GGTI-2154 induces breast carcinoma apoptosis and tumor regression in H-Ras transgenic mice. GGTI-2154 38-47 Harvey rat sarcoma virus oncogene Mus musculus 107-112 15214171-7 2004 Inhibition of Cdks activity with Roscovitine, as well as violation of microtubule depolymerization with nocodazole result in the apoptotic death in the tested cell lines sensitive (E1A + c-Ha-ras) and resistant (E1A + c-Ha-ras + bcl-2) to damaging agents. Nocodazole 104-114 Harvey rat sarcoma virus oncogene Mus musculus 187-195 15214171-7 2004 Inhibition of Cdks activity with Roscovitine, as well as violation of microtubule depolymerization with nocodazole result in the apoptotic death in the tested cell lines sensitive (E1A + c-Ha-ras) and resistant (E1A + c-Ha-ras + bcl-2) to damaging agents. Nocodazole 104-114 Harvey rat sarcoma virus oncogene Mus musculus 218-226 14695209-1 2003 Treatment of H-Ras transgenic mice with the geranylgeranyltransferase I (GGTase I) inhibitor GGTI-2154 results not only in halting the growth of aggressive breast tumors but actually in inducing the regression (54 +/- 3%) of all 19 tumors analyzed. GGTI-2154 93-102 Harvey rat sarcoma virus oncogene Mus musculus 13-18 12850242-5 2003 Supplementation of AC-2-containing medium with growth factors, such as insulin-like growth factor-1 (IGF-1), partially restored the capability of anchorage-independent cell growth induced by Ha-ras overexpression. ac-2-containing medium 19-41 Harvey rat sarcoma virus oncogene Mus musculus 191-197 12958205-1 2003 Stimulation of cannabinoid CB1 receptors by 2-methyl-arachidonyl-2"-fluoro-ethylamide (Met-F-AEA) inhibits the growth of a rat thyroid cancer cell-derived tumor in athymic mice by inhibiting the activity of the oncogene product p21ras. 2-methylarachidonyl-2'-fluoroethylamide 44-85 Harvey rat sarcoma virus oncogene Mus musculus 228-234 12958205-1 2003 Stimulation of cannabinoid CB1 receptors by 2-methyl-arachidonyl-2"-fluoro-ethylamide (Met-F-AEA) inhibits the growth of a rat thyroid cancer cell-derived tumor in athymic mice by inhibiting the activity of the oncogene product p21ras. 2-methylarachidonyl-2'-fluoroethylamide 87-96 Harvey rat sarcoma virus oncogene Mus musculus 228-234 12958205-4 2003 The levels of the cyclin-dependent kinase inhibitor p27(kip1), which is down-regulated by p21ras, were instead increased by Met-F-AEA. 2-methylarachidonyl-2'-fluoroethylamide 124-133 Harvey rat sarcoma virus oncogene Mus musculus 90-96 12915131-0 2003 Ha-ras overexpression mediated cell apoptosis in the presence of 5-fluorouracil. Fluorouracil 65-79 Harvey rat sarcoma virus oncogene Mus musculus 0-6 12915131-1 2003 By using a mouse NIH3T3 derivate designed 7-4 harboring the inducible Ha-ras oncogene, we demonstrated the close relationship between Ha-ras expression level and sensitization of 5-flurouracil (5-FU)-treated cells. 5-flurouracil 179-192 Harvey rat sarcoma virus oncogene Mus musculus 70-76 12915131-1 2003 By using a mouse NIH3T3 derivate designed 7-4 harboring the inducible Ha-ras oncogene, we demonstrated the close relationship between Ha-ras expression level and sensitization of 5-flurouracil (5-FU)-treated cells. 5-flurouracil 179-192 Harvey rat sarcoma virus oncogene Mus musculus 134-140 12915131-1 2003 By using a mouse NIH3T3 derivate designed 7-4 harboring the inducible Ha-ras oncogene, we demonstrated the close relationship between Ha-ras expression level and sensitization of 5-flurouracil (5-FU)-treated cells. Fluorouracil 194-198 Harvey rat sarcoma virus oncogene Mus musculus 70-76 12915131-1 2003 By using a mouse NIH3T3 derivate designed 7-4 harboring the inducible Ha-ras oncogene, we demonstrated the close relationship between Ha-ras expression level and sensitization of 5-flurouracil (5-FU)-treated cells. Fluorouracil 194-198 Harvey rat sarcoma virus oncogene Mus musculus 134-140 12915131-5 2003 These results indicate that Ras, Bcl-2, as well as Raf-1 and PI3K pathways play pivotal roles in 5-FU-induced apoptosis under Ha-ras-overexpressed condition. Fluorouracil 97-101 Harvey rat sarcoma virus oncogene Mus musculus 126-132 12915131-7 2003 Sensitization of Ha-ras-related cells to 5-FU was also demonstrated in human bladder cancer cells. Fluorouracil 41-45 Harvey rat sarcoma virus oncogene Mus musculus 17-23 12915131-8 2003 Through understanding the mechanism of 5-FU induced apoptosis in tumor cells, a new direction toward the treatment of Ha-ras oncogene-related cancers with 5-FU at more optimal dosages is possible and combinational therapy with other drugs that suppress PI3K and Bcl-2 activities can also be considered. Fluorouracil 39-43 Harvey rat sarcoma virus oncogene Mus musculus 118-124 12915131-8 2003 Through understanding the mechanism of 5-FU induced apoptosis in tumor cells, a new direction toward the treatment of Ha-ras oncogene-related cancers with 5-FU at more optimal dosages is possible and combinational therapy with other drugs that suppress PI3K and Bcl-2 activities can also be considered. Fluorouracil 155-159 Harvey rat sarcoma virus oncogene Mus musculus 118-124 12644583-6 2003 The H-Ras+ cells also have enhanced Cx43 promoter activation, which is inhibited by the MEK1 inhibitor 2"-amino-3"-methoxyflavone (PD98059), suggesting that Ras-mediated Cx43 overexpression is via the mitogen activated protein kinase kinase/extracellular signal-regulated pathway. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 103-129 Harvey rat sarcoma virus oncogene Mus musculus 4-9 12642594-0 2003 Distinct rates of palmitate turnover on membrane-bound cellular and oncogenic H-ras. Palmitates 18-27 Harvey rat sarcoma virus oncogene Mus musculus 78-83 12642594-1 2003 H-Ras displays dynamic cycles of GTP binding and palmitate turnover. Guanosine Triphosphate 33-36 Harvey rat sarcoma virus oncogene Mus musculus 0-5 12642594-3 2003 As a way to compare COOH termini of membrane-bound, lipid-modified H-Ras, palmitate removal rates were measured for various forms of H-Ras in NIH 3T3 cells. Palmitates 74-83 Harvey rat sarcoma virus oncogene Mus musculus 133-138 12642594-5 2003 Combining this data with GTP binding measurements, the palmitate half-life of H-Ras in the fully GTP-bound state was estimated to be less than 10 min. Guanosine Triphosphate 25-28 Harvey rat sarcoma virus oncogene Mus musculus 78-83 12642594-5 2003 Combining this data with GTP binding measurements, the palmitate half-life of H-Ras in the fully GTP-bound state was estimated to be less than 10 min. Guanosine Triphosphate 97-100 Harvey rat sarcoma virus oncogene Mus musculus 78-83 12642594-9 2003 Instead, these results suggest that acylprotein thioesterases access oncogenic H-Ras more easily because the conformation of its COOH terminus against the membrane is altered. Carbonic Acid 129-133 Harvey rat sarcoma virus oncogene Mus musculus 79-84 12584202-10 2003 Tyrosine phosphorylation of one Sp3 form was decreased, whereas phosphorylation of two other forms of Sp3 was increased in nuclear extracts of Ha-Ras-transformed cells. Tyrosine 0-8 Harvey rat sarcoma virus oncogene Mus musculus 143-149 12644583-6 2003 The H-Ras+ cells also have enhanced Cx43 promoter activation, which is inhibited by the MEK1 inhibitor 2"-amino-3"-methoxyflavone (PD98059), suggesting that Ras-mediated Cx43 overexpression is via the mitogen activated protein kinase kinase/extracellular signal-regulated pathway. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 131-138 Harvey rat sarcoma virus oncogene Mus musculus 4-9 12555061-5 2003 The basal activation of NF-kappaB in cells expressing H-Ras impaired staurosporine-induced apoptosis in these cells, through a mechanism that was NF-kappaB-dependent and inhibitable in the presence of z-VAD. Staurosporine 69-82 Harvey rat sarcoma virus oncogene Mus musculus 54-59 12618760-0 2003 Glutathione depletion-induced apoptosis of Ha-ras-transformed NIH3T3 cells can be prevented by melatonin. Glutathione 0-11 Harvey rat sarcoma virus oncogene Mus musculus 43-49 12618760-0 2003 Glutathione depletion-induced apoptosis of Ha-ras-transformed NIH3T3 cells can be prevented by melatonin. Melatonin 95-104 Harvey rat sarcoma virus oncogene Mus musculus 43-49 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Isopropyl Thiogalactoside 80-112 Harvey rat sarcoma virus oncogene Mus musculus 61-67 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Isopropyl Thiogalactoside 80-112 Harvey rat sarcoma virus oncogene Mus musculus 239-245 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Isopropyl Thiogalactoside 114-118 Harvey rat sarcoma virus oncogene Mus musculus 61-67 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Isopropyl Thiogalactoside 114-118 Harvey rat sarcoma virus oncogene Mus musculus 239-245 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Reactive Oxygen Species 144-167 Harvey rat sarcoma virus oncogene Mus musculus 61-67 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Reactive Oxygen Species 169-172 Harvey rat sarcoma virus oncogene Mus musculus 61-67 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Reactive Oxygen Species 169-172 Harvey rat sarcoma virus oncogene Mus musculus 239-245 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Superoxides 184-190 Harvey rat sarcoma virus oncogene Mus musculus 61-67 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Hydrogen Peroxide 195-212 Harvey rat sarcoma virus oncogene Mus musculus 61-67 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. h(2) 214-218 Harvey rat sarcoma virus oncogene Mus musculus 61-67 12618760-4 2003 Our results demonstrate that overexpression of the inducible Ha-ras oncogene by isopropyl-beta-D-thiogalactoside (IPTG) increases the levels of reactive oxygen species (ROS, including O(2*-) and hydrogen peroxide (H(2)O(2))) and GSH in an Ha-ras-transformed NIH/3T3 fibroblast cell line. Glutathione 229-232 Harvey rat sarcoma virus oncogene Mus musculus 61-67 12514114-5 2003 We postulate that the immune stimulatory effect of ferrocene is mediated by redox-sensitive signaling such as activation of p21ras. ferrocene 51-60 Harvey rat sarcoma virus oncogene Mus musculus 124-130 12592391-4 2003 This was analysed in detail in a cell line in which the expression of c-Ha-ras and c-myc is under the control of a doxycycline-regulated promoter allowing to switch between the normal and oncogenic cell status. Doxycycline 115-126 Harvey rat sarcoma virus oncogene Mus musculus 70-78 12555061-5 2003 The basal activation of NF-kappaB in cells expressing H-Ras impaired staurosporine-induced apoptosis in these cells, through a mechanism that was NF-kappaB-dependent and inhibitable in the presence of z-VAD. z-vad 201-206 Harvey rat sarcoma virus oncogene Mus musculus 54-59 12555061-6 2003 Moreover, titration of caspase activation in response to staurosporine showed a significant resistance in cells expressing H-Ras when compared with the void vector or the N-Ras counterparts. Staurosporine 57-70 Harvey rat sarcoma virus oncogene Mus musculus 123-128 11884408-3 2002 Expression of dominant-positive H-Ras (V12-H-Ras) enhanced the host cell reactivation of luciferase activity from UV-irradiated and cisplatin-treated plasmids and also increased the unscheduled DNA synthesis following cisplatin or UV treatment of cells. Cisplatin 132-141 Harvey rat sarcoma virus oncogene Mus musculus 32-37 12110620-7 2002 DNA-binding studies confirmed that both AAs bind to the adenines of codon 61 in the H-ras mouse gene and preferentially to purines in the human p53 gene. Adenine 56-64 Harvey rat sarcoma virus oncogene Mus musculus 84-89 12044842-1 2002 Okadaic acid (OA), a tumor promoter in the mouse skin carcinogenesis model, has been shown to induce apoptosis in tumor cell lines that harbor H-ras mutations. Okadaic Acid 0-12 Harvey rat sarcoma virus oncogene Mus musculus 143-148 12044842-1 2002 Okadaic acid (OA), a tumor promoter in the mouse skin carcinogenesis model, has been shown to induce apoptosis in tumor cell lines that harbor H-ras mutations. Okadaic Acid 14-16 Harvey rat sarcoma virus oncogene Mus musculus 143-148 12740648-0 2003 Immunocytochemical detection of p21ras, Raf-1, ERK1/MAP kinase and PKC isoforms in a 20-methylcholanthrene-induced transformed murine embryonal fibroblast cells in culture. Methylcholanthrene 85-106 Harvey rat sarcoma virus oncogene Mus musculus 32-38 12165863-0 2002 Frequent mutations of the Trp53, Hras1 and beta-catenin (Catnb) genes in 1,3-butadiene-induced mammary adenocarcinomas in B6C3F1 mice. 1,3-butadiene 73-86 Harvey rat sarcoma virus oncogene Mus musculus 33-38 12165863-5 2002 Seven of these mutations were a G-->C transversion in Hras1 codon 13, consistent with a 1,3-butadiene-specific Kras2 mutation previously reported in several other tumor types. 1,3-butadiene 91-104 Harvey rat sarcoma virus oncogene Mus musculus 57-62 12165863-5 2002 Seven of these mutations were a G-->C transversion in Hras1 codon 13, consistent with a 1,3-butadiene-specific Kras2 mutation previously reported in several other tumor types. 1,3-butadiene 91-104 Harvey rat sarcoma virus oncogene Mus musculus 114-119 12165863-7 2002 In total, mutations of the Trp53, Hras1 and/or Catnb genes were identified in 15 out of 17 1,3-butadiene-induced mammary adenocarcinomas. 1,3-butadiene 91-104 Harvey rat sarcoma virus oncogene Mus musculus 34-39 12071806-7 2002 We performed a molecular analysis of Hras gene mutations in skin tumors of Car-S mice induced by X-ray initiation/TPA promotion or by TPA promotion alone. Tetradecanoylphorbol Acetate 114-117 Harvey rat sarcoma virus oncogene Mus musculus 37-41 12071806-7 2002 We performed a molecular analysis of Hras gene mutations in skin tumors of Car-S mice induced by X-ray initiation/TPA promotion or by TPA promotion alone. Tetradecanoylphorbol Acetate 134-137 Harvey rat sarcoma virus oncogene Mus musculus 37-41 11884408-3 2002 Expression of dominant-positive H-Ras (V12-H-Ras) enhanced the host cell reactivation of luciferase activity from UV-irradiated and cisplatin-treated plasmids and also increased the unscheduled DNA synthesis following cisplatin or UV treatment of cells. Cisplatin 218-227 Harvey rat sarcoma virus oncogene Mus musculus 32-37 11884408-3 2002 Expression of dominant-positive H-Ras (V12-H-Ras) enhanced the host cell reactivation of luciferase activity from UV-irradiated and cisplatin-treated plasmids and also increased the unscheduled DNA synthesis following cisplatin or UV treatment of cells. Cisplatin 218-227 Harvey rat sarcoma virus oncogene Mus musculus 43-48 11884408-7 2002 We found that ROS production by V12-H-Ras expression was mediated by the Ras/phosphatidylinositol 3-kinase (PI3K)/Rac1/NADPH oxidase-dependent pathway and that pretreatment of V12-H-Ras-transformed cells with an antioxidant (N-acetylcysteine) and an NADPH oxidase inhibitor (diphenyleneiodonium) decreased DNA repair capacity. Reactive Oxygen Species 14-17 Harvey rat sarcoma virus oncogene Mus musculus 36-41 11884408-7 2002 We found that ROS production by V12-H-Ras expression was mediated by the Ras/phosphatidylinositol 3-kinase (PI3K)/Rac1/NADPH oxidase-dependent pathway and that pretreatment of V12-H-Ras-transformed cells with an antioxidant (N-acetylcysteine) and an NADPH oxidase inhibitor (diphenyleneiodonium) decreased DNA repair capacity. Reactive Oxygen Species 14-17 Harvey rat sarcoma virus oncogene Mus musculus 180-185 11884408-7 2002 We found that ROS production by V12-H-Ras expression was mediated by the Ras/phosphatidylinositol 3-kinase (PI3K)/Rac1/NADPH oxidase-dependent pathway and that pretreatment of V12-H-Ras-transformed cells with an antioxidant (N-acetylcysteine) and an NADPH oxidase inhibitor (diphenyleneiodonium) decreased DNA repair capacity. Acetylcysteine 225-241 Harvey rat sarcoma virus oncogene Mus musculus 36-41 11884408-3 2002 Expression of dominant-positive H-Ras (V12-H-Ras) enhanced the host cell reactivation of luciferase activity from UV-irradiated and cisplatin-treated plasmids and also increased the unscheduled DNA synthesis following cisplatin or UV treatment of cells. Cisplatin 132-141 Harvey rat sarcoma virus oncogene Mus musculus 43-48 11884408-7 2002 We found that ROS production by V12-H-Ras expression was mediated by the Ras/phosphatidylinositol 3-kinase (PI3K)/Rac1/NADPH oxidase-dependent pathway and that pretreatment of V12-H-Ras-transformed cells with an antioxidant (N-acetylcysteine) and an NADPH oxidase inhibitor (diphenyleneiodonium) decreased DNA repair capacity. diphenyleneiodonium 275-294 Harvey rat sarcoma virus oncogene Mus musculus 36-41 11884408-8 2002 Similarly, treatment with PI3K inhibitors (wortmannin and LY294002) inhibited the ability of oncogenic H-Ras to enhance DNA repair capacity. Wortmannin 43-53 Harvey rat sarcoma virus oncogene Mus musculus 103-108 11884408-8 2002 Similarly, treatment with PI3K inhibitors (wortmannin and LY294002) inhibited the ability of oncogenic H-Ras to enhance DNA repair capacity. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 58-66 Harvey rat sarcoma virus oncogene Mus musculus 103-108 11884408-9 2002 Furthermore, inhibition of the Ras/PI3K/Rac1/NADPH oxidase pathway resulted in increased sensitivity to cisplatin and UV in V12-H-Ras-expressing NIH3T3 cells. Cisplatin 104-113 Harvey rat sarcoma virus oncogene Mus musculus 128-133 11884408-10 2002 Taken together, these results provide evidence that oncogenic H-Ras activates DNA repair capacity through the Ras/PI3K/Rac1/NADPH oxidase-dependent pathway and that increased ROS production via this signaling pathway is required for enhancement of the DNA repair capacity induced by oncogenic H-Ras. Reactive Oxygen Species 175-178 Harvey rat sarcoma virus oncogene Mus musculus 62-67 11884408-10 2002 Taken together, these results provide evidence that oncogenic H-Ras activates DNA repair capacity through the Ras/PI3K/Rac1/NADPH oxidase-dependent pathway and that increased ROS production via this signaling pathway is required for enhancement of the DNA repair capacity induced by oncogenic H-Ras. Reactive Oxygen Species 175-178 Harvey rat sarcoma virus oncogene Mus musculus 293-298 11731413-5 2001 Because 7,12-dimethylbenz[a]anthracene induces an activating mutation of H-ras, this provides one possible explanation for suppression of papilloma formation when FAK protein is limiting. 7,12-dimethylbenz[a] 8-28 Harvey rat sarcoma virus oncogene Mus musculus 73-78 12016150-0 2002 Sodium arsenite administration via drinking water increases genome-wide and Ha-ras DNA hypomethylation in methyl-deficient C57BL/6J mice. sodium arsenite 0-15 Harvey rat sarcoma virus oncogene Mus musculus 76-82 12016150-0 2002 Sodium arsenite administration via drinking water increases genome-wide and Ha-ras DNA hypomethylation in methyl-deficient C57BL/6J mice. Water 44-49 Harvey rat sarcoma virus oncogene Mus musculus 76-82 12016150-8 2002 Sodium arsenite increased genomic hypomethylation in a dose dependent manner and methyl-deficiency and sodium arsenite reduced the frequency of methylation at several cytosine sites within the promoter region of the oncogenic gene, Ha-ras. sodium arsenite 0-15 Harvey rat sarcoma virus oncogene Mus musculus 232-238 12016150-8 2002 Sodium arsenite increased genomic hypomethylation in a dose dependent manner and methyl-deficiency and sodium arsenite reduced the frequency of methylation at several cytosine sites within the promoter region of the oncogenic gene, Ha-ras. sodium arsenite 103-118 Harvey rat sarcoma virus oncogene Mus musculus 232-238 12016150-8 2002 Sodium arsenite increased genomic hypomethylation in a dose dependent manner and methyl-deficiency and sodium arsenite reduced the frequency of methylation at several cytosine sites within the promoter region of the oncogenic gene, Ha-ras. Cytosine 167-175 Harvey rat sarcoma virus oncogene Mus musculus 232-238 12016150-11 2002 Significantly reduced methylation at a key regulatory region of Ha-ras in the mouse liver may have relevance to understanding arsenic-induced perturbations in the methylation patterns of cellular growth genes involved in the formation of tumors. Arsenic 126-133 Harvey rat sarcoma virus oncogene Mus musculus 64-70 11921187-2 2002 In the mouse model, the most frequently used chemical carcinogen has been 7,12-dimethylbenz[a]anthracene (DMBA), which activates the Ha-ras gene but does not alter the p53 tumor-suppressor gene. 7,12-dimethylbenz[a] 74-94 Harvey rat sarcoma virus oncogene Mus musculus 133-139 11921187-2 2002 In the mouse model, the most frequently used chemical carcinogen has been 7,12-dimethylbenz[a]anthracene (DMBA), which activates the Ha-ras gene but does not alter the p53 tumor-suppressor gene. anthracene 94-104 Harvey rat sarcoma virus oncogene Mus musculus 133-139 11921187-2 2002 In the mouse model, the most frequently used chemical carcinogen has been 7,12-dimethylbenz[a]anthracene (DMBA), which activates the Ha-ras gene but does not alter the p53 tumor-suppressor gene. 9,10-Dimethyl-1,2-benzanthracene 106-110 Harvey rat sarcoma virus oncogene Mus musculus 133-139 11731413-5 2001 Because 7,12-dimethylbenz[a]anthracene induces an activating mutation of H-ras, this provides one possible explanation for suppression of papilloma formation when FAK protein is limiting. anthracene 28-38 Harvey rat sarcoma virus oncogene Mus musculus 73-78 11753661-6 2001 In tumors from mice treated with DEN alone, the prevalence of Ha-ras mutations was approximately 30% (6/20), while no beta-catenin mutations (0/13) were detectable in tumors of this treatment group. Diethylnitrosamine 33-36 Harvey rat sarcoma virus oncogene Mus musculus 62-68 11753677-1 2001 Treatment of SENCAR mouse skin with dibenzo[a,l]pyrene results in abundant formation of abasic sites that undergo error-prone excision repair, forming oncogenic H-ras mutations in the early preneoplastic period. dibenzo(a,l)pyrene 36-54 Harvey rat sarcoma virus oncogene Mus musculus 161-166 11749692-0 2001 p53 and H-ras mutations and microsatellite instability in renal pelvic carcinomas of NON / Shi mice treated with N-butyl-N-(4-hydroxybutyl)-nitrosamine: different genetic alteration from urinary bladder carcinoma. Butylhydroxybutylnitrosamine 113-151 Harvey rat sarcoma virus oncogene Mus musculus 8-13 11753677-4 2001 Between 6 h and 3 days, E(2)-3,4-Q induced abundant A to G mutations in H-ras DNA, frequently in the context of a 3"-G residue. Estradiol 24-29 Harvey rat sarcoma virus oncogene Mus musculus 72-77 11753677-4 2001 Between 6 h and 3 days, E(2)-3,4-Q induced abundant A to G mutations in H-ras DNA, frequently in the context of a 3"-G residue. 3,4-q 29-34 Harvey rat sarcoma virus oncogene Mus musculus 72-77 11753661-7 2001 By contrast, Ha-ras mutations were undetectable in tumors from mice treated with DEN/PB (0/32), while approximately 80% (37/46) of tumors from this group showed beta-catenin mutations. Diethylnitrosamine 81-84 Harvey rat sarcoma virus oncogene Mus musculus 13-19 11753661-7 2001 By contrast, Ha-ras mutations were undetectable in tumors from mice treated with DEN/PB (0/32), while approximately 80% (37/46) of tumors from this group showed beta-catenin mutations. Phenobarbital 85-87 Harvey rat sarcoma virus oncogene Mus musculus 13-19 11256959-0 2001 Oxidative modification of H-ras: S-thiolation and S-nitrosylation of reactive cysteines. reactive 69-77 Harvey rat sarcoma virus oncogene Mus musculus 26-31 11572997-0 2001 Avicins, a family of triterpenoid saponins from Acacia victoriae (Bentham), suppress H-ras mutations and aneuploidy in a murine skin carcinogenesis model. avicins 0-7 Harvey rat sarcoma virus oncogene Mus musculus 85-90 11572997-6 2001 We also observed a 62% and 74% reduction by avicins in H-ras mutations at codon 61 in the DMBA and DMBA/TPA models, respectively, as well as a significant inhibition of the modified DNA base formation (8-OH-dG) in both protocols. avicins 44-51 Harvey rat sarcoma virus oncogene Mus musculus 55-60 11572997-6 2001 We also observed a 62% and 74% reduction by avicins in H-ras mutations at codon 61 in the DMBA and DMBA/TPA models, respectively, as well as a significant inhibition of the modified DNA base formation (8-OH-dG) in both protocols. 9,10-Dimethyl-1,2-benzanthracene 90-94 Harvey rat sarcoma virus oncogene Mus musculus 55-60 11572997-6 2001 We also observed a 62% and 74% reduction by avicins in H-ras mutations at codon 61 in the DMBA and DMBA/TPA models, respectively, as well as a significant inhibition of the modified DNA base formation (8-OH-dG) in both protocols. 9,10-Dimethyl-1,2-benzanthracene 99-103 Harvey rat sarcoma virus oncogene Mus musculus 55-60 11572997-6 2001 We also observed a 62% and 74% reduction by avicins in H-ras mutations at codon 61 in the DMBA and DMBA/TPA models, respectively, as well as a significant inhibition of the modified DNA base formation (8-OH-dG) in both protocols. Tetradecanoylphorbol Acetate 104-107 Harvey rat sarcoma virus oncogene Mus musculus 55-60 11397402-8 2001 These data suggest that the epoxide intermediates of the structurally related chemicals (BD, IP, and CP) may cause DNA damage in K-ras and H-ras proto-oncogenes of B6C3F1 mice following inhalation exposure and that mutational activation of these genes may be critical events in the pathogenesis of forestomach neoplasms induced in the B6C3F1 mouse. Epoxy Compounds 28-35 Harvey rat sarcoma virus oncogene Mus musculus 139-144 11256959-0 2001 Oxidative modification of H-ras: S-thiolation and S-nitrosylation of reactive cysteines. Cysteine 78-87 Harvey rat sarcoma virus oncogene Mus musculus 26-31 11256959-1 2001 The reactive cysteines in H-ras are subject to oxidative modifications that potentially alter the cellular function of this protein. Cysteine 13-22 Harvey rat sarcoma virus oncogene Mus musculus 26-31 11256959-2 2001 In this study, purified H-ras was modified by thiol oxidants such as hydrogen peroxide (H(2)O(2)), S-nitrosoglutathione, diamide, glutathione disulphide (GSSG) and cystamine, producing as many as four charge-isomeric forms of the protein. Sulfhydryl Compounds 46-51 Harvey rat sarcoma virus oncogene Mus musculus 24-29 11256959-2 2001 In this study, purified H-ras was modified by thiol oxidants such as hydrogen peroxide (H(2)O(2)), S-nitrosoglutathione, diamide, glutathione disulphide (GSSG) and cystamine, producing as many as four charge-isomeric forms of the protein. Hydrogen Peroxide 69-86 Harvey rat sarcoma virus oncogene Mus musculus 24-29 11256959-2 2001 In this study, purified H-ras was modified by thiol oxidants such as hydrogen peroxide (H(2)O(2)), S-nitrosoglutathione, diamide, glutathione disulphide (GSSG) and cystamine, producing as many as four charge-isomeric forms of the protein. S-Nitrosoglutathione 99-119 Harvey rat sarcoma virus oncogene Mus musculus 24-29 11256959-2 2001 In this study, purified H-ras was modified by thiol oxidants such as hydrogen peroxide (H(2)O(2)), S-nitrosoglutathione, diamide, glutathione disulphide (GSSG) and cystamine, producing as many as four charge-isomeric forms of the protein. Diamide 121-128 Harvey rat sarcoma virus oncogene Mus musculus 24-29 11256959-2 2001 In this study, purified H-ras was modified by thiol oxidants such as hydrogen peroxide (H(2)O(2)), S-nitrosoglutathione, diamide, glutathione disulphide (GSSG) and cystamine, producing as many as four charge-isomeric forms of the protein. Glutathione Disulfide 130-152 Harvey rat sarcoma virus oncogene Mus musculus 24-29 11256959-2 2001 In this study, purified H-ras was modified by thiol oxidants such as hydrogen peroxide (H(2)O(2)), S-nitrosoglutathione, diamide, glutathione disulphide (GSSG) and cystamine, producing as many as four charge-isomeric forms of the protein. Glutathione Disulfide 154-158 Harvey rat sarcoma virus oncogene Mus musculus 24-29 11746817-0 2001 Comparison of Ha-ras mutational spectra of N-methyldibenzo[c,g]carbazole and 7H-dibenzo[c,g]carbazole-induced mouse skin tumors. N-Methyl-7H-dibenzo(c,g)carbazole 43-72 Harvey rat sarcoma virus oncogene Mus musculus 14-20 11746817-0 2001 Comparison of Ha-ras mutational spectra of N-methyldibenzo[c,g]carbazole and 7H-dibenzo[c,g]carbazole-induced mouse skin tumors. 7H-dibenzo(c,g)carbazole 77-101 Harvey rat sarcoma virus oncogene Mus musculus 14-20 11453734-0 2001 trans-3,4-dihydroxy-anti-1,2-epoxy-1,2,3,4-tetrahydrodi- benz[a,j]acridine involvement in dibenz[a,j]acridine DNA adduct formation in mouse skin consistent with Ha-ras mutation patterns in tumors. Acridines 66-74 Harvey rat sarcoma virus oncogene Mus musculus 161-167 11453734-0 2001 trans-3,4-dihydroxy-anti-1,2-epoxy-1,2,3,4-tetrahydrodi- benz[a,j]acridine involvement in dibenz[a,j]acridine DNA adduct formation in mouse skin consistent with Ha-ras mutation patterns in tumors. Acridines 101-109 Harvey rat sarcoma virus oncogene Mus musculus 161-167 11453734-3 2001 The present studies were undertaken to determine the role of trans-3,4-dihydroxy-anti-1,2-epoxy-1,2,3,4-tetrahydroDBA (DBADE) in DBA activation pathway(s), the DNA bases involved in the binding of DBA to DNA, and whether the adducts produced are consistent with the mutation pattern in the Ha-ras gene. dibenz(a,h)anthracene-3,4-diol 1,2-oxide 119-124 Harvey rat sarcoma virus oncogene Mus musculus 290-296 11453734-3 2001 The present studies were undertaken to determine the role of trans-3,4-dihydroxy-anti-1,2-epoxy-1,2,3,4-tetrahydroDBA (DBADE) in DBA activation pathway(s), the DNA bases involved in the binding of DBA to DNA, and whether the adducts produced are consistent with the mutation pattern in the Ha-ras gene. dibenzacridine 114-117 Harvey rat sarcoma virus oncogene Mus musculus 290-296 11453734-3 2001 The present studies were undertaken to determine the role of trans-3,4-dihydroxy-anti-1,2-epoxy-1,2,3,4-tetrahydroDBA (DBADE) in DBA activation pathway(s), the DNA bases involved in the binding of DBA to DNA, and whether the adducts produced are consistent with the mutation pattern in the Ha-ras gene. dibenzacridine 119-122 Harvey rat sarcoma virus oncogene Mus musculus 290-296 11453734-11 2001 The mutational spectra in the Ha-ras gene are consistent with the DNA binding of DBA to dG or dA in vivo. dibenzacridine 81-84 Harvey rat sarcoma virus oncogene Mus musculus 30-36 11439698-6 2001 Second, H-RAS transformed solid tumor cells were pretreated in vitro with normal oligonucleotides, after which tumor growth from the treated cells was tested in nude mice. Oligonucleotides 81-97 Harvey rat sarcoma virus oncogene Mus musculus 8-13 11439698-8 2001 Tumor growth of cells treated with H-RAS antisense oligonucleotide was significantly reduced for up to 14 d following the end of treatment and implantation into the mice, whereas the nonspecific control DNA had no significant effect. Oligonucleotides 51-66 Harvey rat sarcoma virus oncogene Mus musculus 35-40 11439698-9 2001 Third, H-RAS transformed bladder cancer cells were implanted into nude mice, after which the mice were treated for 31 d with oligonucleotide phosphorothioates. Phosphorothioate Oligonucleotides 125-158 Harvey rat sarcoma virus oncogene Mus musculus 7-12 11439698-10 2001 Tumor growth in mice treated with H-RAS 12th codon antisense oligonucleotide was reduced by about 80% throughout the treatment period, reiterating the sustained effect seen in pretreated tumor cells. Oligonucleotides 61-76 Harvey rat sarcoma virus oncogene Mus musculus 34-39 11439698-15 2001 Tumor growth in mice treated with K-RAS 5"-UTR antisense oligonucleotide was reduced by about 50% throughout the treatment period, reiterating the sustained effect seen with H-RAS transformed cells. Oligonucleotides 57-72 Harvey rat sarcoma virus oncogene Mus musculus 174-179 11256959-2 2001 In this study, purified H-ras was modified by thiol oxidants such as hydrogen peroxide (H(2)O(2)), S-nitrosoglutathione, diamide, glutathione disulphide (GSSG) and cystamine, producing as many as four charge-isomeric forms of the protein. Cystamine 164-173 Harvey rat sarcoma virus oncogene Mus musculus 24-29 11256959-3 2001 These results suggest that all four reactive cysteines of H-ras are potential sites of regulatory modification reactions. Cysteine 45-54 Harvey rat sarcoma virus oncogene Mus musculus 58-63 11451381-0 2001 Phorbol ester tumour promoter mediated altered expression and regulation of matrix metalloproteinase-2 in a H-ras transformed cell line capable of benign tumour formation. Phorbol Esters 0-13 Harvey rat sarcoma virus oncogene Mus musculus 108-113 11256959-5 2001 S-nitrosoglutathione could S-nitrosylate H-ras on four cysteine residues, while reduced glutathione (GSH) and H(2)O(2) mediate S-glutathiolation on at least one cysteine of H-ras. S-Nitrosoglutathione 0-20 Harvey rat sarcoma virus oncogene Mus musculus 41-46 11256959-5 2001 S-nitrosoglutathione could S-nitrosylate H-ras on four cysteine residues, while reduced glutathione (GSH) and H(2)O(2) mediate S-glutathiolation on at least one cysteine of H-ras. S-Nitrosoglutathione 0-20 Harvey rat sarcoma virus oncogene Mus musculus 173-178 11256959-5 2001 S-nitrosoglutathione could S-nitrosylate H-ras on four cysteine residues, while reduced glutathione (GSH) and H(2)O(2) mediate S-glutathiolation on at least one cysteine of H-ras. Cysteine 55-63 Harvey rat sarcoma virus oncogene Mus musculus 41-46 11256959-5 2001 S-nitrosoglutathione could S-nitrosylate H-ras on four cysteine residues, while reduced glutathione (GSH) and H(2)O(2) mediate S-glutathiolation on at least one cysteine of H-ras. Glutathione 9-20 Harvey rat sarcoma virus oncogene Mus musculus 41-46 11256959-5 2001 S-nitrosoglutathione could S-nitrosylate H-ras on four cysteine residues, while reduced glutathione (GSH) and H(2)O(2) mediate S-glutathiolation on at least one cysteine of H-ras. Glutathione 9-20 Harvey rat sarcoma virus oncogene Mus musculus 173-178 11256959-6 2001 Either GSSG or diamide S-glutathiolated at least two cysteine residues of purified H-ras. Glutathione Disulfide 7-11 Harvey rat sarcoma virus oncogene Mus musculus 83-88 11256959-6 2001 Either GSSG or diamide S-glutathiolated at least two cysteine residues of purified H-ras. Diamide 15-22 Harvey rat sarcoma virus oncogene Mus musculus 83-88 11256959-6 2001 Either GSSG or diamide S-glutathiolated at least two cysteine residues of purified H-ras. Cysteine 53-61 Harvey rat sarcoma virus oncogene Mus musculus 83-88 11256959-8 2001 In intact NIH-3T3 cells, wild-type H-ras was S-glutathiolated by diamide. Diamide 65-72 Harvey rat sarcoma virus oncogene Mus musculus 35-40 11256959-9 2001 Similarly, cells expressing a C118S mutant or a C181S/C184S double mutant of H-ras were S-glutathiolated by diamide. Diamide 108-115 Harvey rat sarcoma virus oncogene Mus musculus 77-82 11256959-10 2001 These results suggest that H-ras can be S-glutathiolated on multiple thiols in vivo and that at least one of these thiols is normally lipid-modified. Sulfhydryl Compounds 69-75 Harvey rat sarcoma virus oncogene Mus musculus 27-32 11256959-10 2001 These results suggest that H-ras can be S-glutathiolated on multiple thiols in vivo and that at least one of these thiols is normally lipid-modified. Sulfhydryl Compounds 115-121 Harvey rat sarcoma virus oncogene Mus musculus 27-32 11256959-11 2001 In cells treated with S-nitrosocysteine, evidence for both S-nitrosylated and S-glutathiolated H-ras was obtained and S-nitrosylation was the predominant modification. S-nitrosocysteine 22-39 Harvey rat sarcoma virus oncogene Mus musculus 95-100 11256959-12 2001 These results show that oxidative modification of H-ras can be extensive in vivo, that both S-nitrosylated and S-glutathiolated forms may be important, and that oxidation may occur on reactive cysteines that are normally targeted for lipid-modification reactions. Cysteine 193-202 Harvey rat sarcoma virus oncogene Mus musculus 50-55 11259608-17 2001 In fact, in cisplatin-resistant keratinocytes transformed by H-ras oncogene, a high dose of cisplatin (312 microM) induces characteristic features of necrotic cell death(Perez et al., 1999). Cisplatin 12-21 Harvey rat sarcoma virus oncogene Mus musculus 61-66 11259608-17 2001 In fact, in cisplatin-resistant keratinocytes transformed by H-ras oncogene, a high dose of cisplatin (312 microM) induces characteristic features of necrotic cell death(Perez et al., 1999). Cisplatin 92-101 Harvey rat sarcoma virus oncogene Mus musculus 61-66 11451381-2 2001 The present study demonstrates alterations in the regulation of matrix metalloproteinase-2 (MMP-2) expression in response to the phorbol ester tumour promoter, PMA, in a H-ras transformed cell line, NR3, which is capable of benign tumour formation. Phorbol Esters 129-142 Harvey rat sarcoma virus oncogene Mus musculus 170-175 11280800-0 2001 The farnesyltransferase inhibitor L744,832 reduces hypoxia in tumors expressing activated H-ras. 4-Methoxyphenethyl alcohol 34-38 Harvey rat sarcoma virus oncogene Mus musculus 90-95 11087892-0 2000 Evidence that error-prone DNA repair converts dibenzo[a,l]pyrene-induced depurinating lesions into mutations: formation, clonal proliferation and regression of initiated cells carrying H-ras oncogene mutations in early preneoplasia. dibenzo(a,l)pyrene 46-64 Harvey rat sarcoma virus oncogene Mus musculus 185-190 11258968-2 2001 Recent studies support depurination of DNA as a mechanism central to the genesis of H-ras mutations in PAH-treated mouse skin. Polycyclic Aromatic Hydrocarbons 103-106 Harvey rat sarcoma virus oncogene Mus musculus 84-89 11241758-3 2001 The data showed that a A(182)-->T transversion in the c-Ha-ras gene was present in 100% and 81% of the skin tumors developed in Car-S and Car-R mice, respectively, after DMBA initiation and TPA promotion, suggesting that differences in genetic susceptibility can influence the frequency of c-Ha-ras mutations in the skin tumors produced. 9,10-Dimethyl-1,2-benzanthracene 173-177 Harvey rat sarcoma virus oncogene Mus musculus 57-65 11241758-3 2001 The data showed that a A(182)-->T transversion in the c-Ha-ras gene was present in 100% and 81% of the skin tumors developed in Car-S and Car-R mice, respectively, after DMBA initiation and TPA promotion, suggesting that differences in genetic susceptibility can influence the frequency of c-Ha-ras mutations in the skin tumors produced. Tetradecanoylphorbol Acetate 193-196 Harvey rat sarcoma virus oncogene Mus musculus 57-65 11235918-10 2001 The status of the cellular polyamine levels was also an important determinant of the PMA-mediated alterations that occurred in ODC and in SAMDC expression in these H-ras transformed cells. Polyamines 27-36 Harvey rat sarcoma virus oncogene Mus musculus 164-169 11231886-0 2001 Inhibition of carcinogen induced c-Ha-ras and c-fos proto-oncogenes expression by dietary curcumin. Curcumin 90-98 Harvey rat sarcoma virus oncogene Mus musculus 33-41 11238813-2 2001 Oral administration of diallyl disulfide resulted in a dose-dependent and significant inhibition of the growth of H-ras oncogene transformed NIH 3T3 cells implanted in nude mice. diallyl disulfide 23-40 Harvey rat sarcoma virus oncogene Mus musculus 114-119 11238813-4 2001 On the other hand, the growth of H-ras oncogene transformed tumors was not inhibited by dipropyl disulfide, a naturally occurring saturated analog of diallyl disulfide. diallyl disulfide 150-167 Harvey rat sarcoma virus oncogene Mus musculus 33-38 11238813-5 2001 The diallyl disulfide-mediated inhibition of H-ras oncogene transformed tumor growth correlated with the inhibition of p21(H-ras) membrane association. diallyl disulfide 4-21 Harvey rat sarcoma virus oncogene Mus musculus 45-50 11238813-5 2001 The diallyl disulfide-mediated inhibition of H-ras oncogene transformed tumor growth correlated with the inhibition of p21(H-ras) membrane association. diallyl disulfide 4-21 Harvey rat sarcoma virus oncogene Mus musculus 123-128 11238813-6 2001 The levels of membrane-associated p21(H-ras) were markedly lower in the tumors of diallyl disulfide-treated mice than in those of controls. diallyl disulfide 82-99 Harvey rat sarcoma virus oncogene Mus musculus 38-43 11238813-8 2001 The results of this study indicate that diallyl disulfide inhibits the growth of H-ras oncogene transformed tumors in vivo by inhibiting the membrane association of p21(H-ras) and that the allyl group may be an important determinant in the inhibitory effect of this organosulfide on tumor growth. diallyl disulfide 40-57 Harvey rat sarcoma virus oncogene Mus musculus 81-86 11238813-8 2001 The results of this study indicate that diallyl disulfide inhibits the growth of H-ras oncogene transformed tumors in vivo by inhibiting the membrane association of p21(H-ras) and that the allyl group may be an important determinant in the inhibitory effect of this organosulfide on tumor growth. diallyl disulfide 40-57 Harvey rat sarcoma virus oncogene Mus musculus 169-174 11238813-8 2001 The results of this study indicate that diallyl disulfide inhibits the growth of H-ras oncogene transformed tumors in vivo by inhibiting the membrane association of p21(H-ras) and that the allyl group may be an important determinant in the inhibitory effect of this organosulfide on tumor growth. organosulfide 266-279 Harvey rat sarcoma virus oncogene Mus musculus 81-86 11180512-0 2001 Interferon-gamma protects astrocytes from apoptosis and increases the formation of p21ras-GTP complex through ras oncogene family overexpression. Guanosine Triphosphate 90-93 Harvey rat sarcoma virus oncogene Mus musculus 83-89 11087892-2 2000 Mice treated on the dorsal skin with the potent polycyclic aromatic hydrocarbon (PAH) carcinogen dibenzo[a,l]pyrene (DB[a,l]P) form papillomas carrying the H-ras codon 61 (CAA to CTA) mutations. Polycyclic Aromatic Hydrocarbons 48-79 Harvey rat sarcoma virus oncogene Mus musculus 156-161 11087892-2 2000 Mice treated on the dorsal skin with the potent polycyclic aromatic hydrocarbon (PAH) carcinogen dibenzo[a,l]pyrene (DB[a,l]P) form papillomas carrying the H-ras codon 61 (CAA to CTA) mutations. Polycyclic Aromatic Hydrocarbons 81-84 Harvey rat sarcoma virus oncogene Mus musculus 156-161 11087892-2 2000 Mice treated on the dorsal skin with the potent polycyclic aromatic hydrocarbon (PAH) carcinogen dibenzo[a,l]pyrene (DB[a,l]P) form papillomas carrying the H-ras codon 61 (CAA to CTA) mutations. dibenzo(a,l)pyrene 97-115 Harvey rat sarcoma virus oncogene Mus musculus 156-161 11059761-0 2000 Both (+/-)syn- and (+/-)anti-7,12-dimethylbenz[a]anthracene-3,4-diol-1,2-epoxides initiate tumors in mouse skin that possess -CAA- to -CTA- mutations at Codon 61 of c-H-ras. 7,12-dimethylbenz 29-46 Harvey rat sarcoma virus oncogene Mus musculus 165-172 11059761-0 2000 Both (+/-)syn- and (+/-)anti-7,12-dimethylbenz[a]anthracene-3,4-diol-1,2-epoxides initiate tumors in mouse skin that possess -CAA- to -CTA- mutations at Codon 61 of c-H-ras. anthracene-3,4-diol-1,2-epoxides 49-81 Harvey rat sarcoma virus oncogene Mus musculus 165-172 11059761-7 2000 Previously, we showed that both the syn- and anti-DMBADE bind to the adenine (A182) at codon 61 of H-ras. dmbade 50-56 Harvey rat sarcoma virus oncogene Mus musculus 99-104 11059761-7 2000 Previously, we showed that both the syn- and anti-DMBADE bind to the adenine (A182) at codon 61 of H-ras. Adenine 69-76 Harvey rat sarcoma virus oncogene Mus musculus 99-104 11059761-7 2000 Previously, we showed that both the syn- and anti-DMBADE bind to the adenine (A182) at codon 61 of H-ras. 3-hydroxybutanohydrazide 78-82 Harvey rat sarcoma virus oncogene Mus musculus 99-104 11059761-8 2000 Collectively, these results indicate that the adenine adducts induced by both bay-region diol epoxides of DMBA lead to the mutation at codon 61 of H-ras and, consequently, initiate tumorigenesis in mouse skin. Adenine 46-53 Harvey rat sarcoma virus oncogene Mus musculus 147-152 11059761-8 2000 Collectively, these results indicate that the adenine adducts induced by both bay-region diol epoxides of DMBA lead to the mutation at codon 61 of H-ras and, consequently, initiate tumorigenesis in mouse skin. diol epoxides 89-102 Harvey rat sarcoma virus oncogene Mus musculus 147-152 11059761-8 2000 Collectively, these results indicate that the adenine adducts induced by both bay-region diol epoxides of DMBA lead to the mutation at codon 61 of H-ras and, consequently, initiate tumorigenesis in mouse skin. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 106-110 Harvey rat sarcoma virus oncogene Mus musculus 147-152 10896935-4 2000 Here, we demonstrate that the expression of the dominant active mutant of Ha-Ras (Ha-Ras(Val-12)) induces redistribution of CD44 to the newly generated membrane ruffling area and CD44 ectodomain cleavage. val-12 89-95 Harvey rat sarcoma virus oncogene Mus musculus 74-80 10896935-6 2000 Treatment with LY294002, an inhibitor for phosphoinositide 3-OH kinase (PI3K), significantly inhibits Ha-Ras(Val-12)-induced CD44 cleavage, whereas that with PD98059, an inhibitor for MEK, does not. val-12 109-115 Harvey rat sarcoma virus oncogene Mus musculus 102-108 10896935-4 2000 Here, we demonstrate that the expression of the dominant active mutant of Ha-Ras (Ha-Ras(Val-12)) induces redistribution of CD44 to the newly generated membrane ruffling area and CD44 ectodomain cleavage. val-12 89-95 Harvey rat sarcoma virus oncogene Mus musculus 82-88 10896935-8 2000 Moreover, the expression of dominant negative mutants of Cdc42 and Rac1 inhibits the Ha-Ras(Val-12)-induced CD44 cleavage. val-12 92-98 Harvey rat sarcoma virus oncogene Mus musculus 85-91 10896935-5 2000 The migration assay revealed that the CD44 cleavage contributes to the Ha-Ras(Val-12)-induced migration of NIH3T3 cells on hyaluronate substrate. val-12 78-84 Harvey rat sarcoma virus oncogene Mus musculus 71-77 10896935-5 2000 The migration assay revealed that the CD44 cleavage contributes to the Ha-Ras(Val-12)-induced migration of NIH3T3 cells on hyaluronate substrate. hyaluronate 123-134 Harvey rat sarcoma virus oncogene Mus musculus 71-77 10896935-6 2000 Treatment with LY294002, an inhibitor for phosphoinositide 3-OH kinase (PI3K), significantly inhibits Ha-Ras(Val-12)-induced CD44 cleavage, whereas that with PD98059, an inhibitor for MEK, does not. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 15-23 Harvey rat sarcoma virus oncogene Mus musculus 102-108 10898794-7 2000 Early-passage DMP1-null cells, like MEFs from either ARF-null or p53-null mice, can be morphologically transformed by oncogenic Ha-Ras (Val-12) alone. Valine 136-139 Harvey rat sarcoma virus oncogene Mus musculus 128-134 10945634-3 2000 R- cells stably transfected with an activated Ha-Ras (R-Ras cells) fail to form colonies in soft agar. Agar 97-101 Harvey rat sarcoma virus oncogene Mus musculus 46-52 10801823-1 2000 Ha-Ras is modified by isoprenoid on Cys(186) and by reversibly attached palmitates at Cys(181) and Cys(184). Terpenes 22-32 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-1 2000 Ha-Ras is modified by isoprenoid on Cys(186) and by reversibly attached palmitates at Cys(181) and Cys(184). Cysteine 36-39 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-1 2000 Ha-Ras is modified by isoprenoid on Cys(186) and by reversibly attached palmitates at Cys(181) and Cys(184). Palmitates 72-82 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-1 2000 Ha-Ras is modified by isoprenoid on Cys(186) and by reversibly attached palmitates at Cys(181) and Cys(184). Cysteine 86-89 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-1 2000 Ha-Ras is modified by isoprenoid on Cys(186) and by reversibly attached palmitates at Cys(181) and Cys(184). Cysteine 86-89 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-2 2000 Ha-Ras loses 90% of its transforming activity if Cys(181) and Cys(184) are changed to serines, implying that palmitates make important contributions to oncogenicity. Cysteine 49-52 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-2 2000 Ha-Ras loses 90% of its transforming activity if Cys(181) and Cys(184) are changed to serines, implying that palmitates make important contributions to oncogenicity. Cysteine 62-65 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-2 2000 Ha-Ras loses 90% of its transforming activity if Cys(181) and Cys(184) are changed to serines, implying that palmitates make important contributions to oncogenicity. Serine 86-93 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-2 2000 Ha-Ras loses 90% of its transforming activity if Cys(181) and Cys(184) are changed to serines, implying that palmitates make important contributions to oncogenicity. Palmitates 109-119 Harvey rat sarcoma virus oncogene Mus musculus 0-6 10801823-4 2000 S-nitrosocysteine (SNC) and, to a more modest extent, S-nitrosoglutathione were found to rapidly increase [(3)H]palmitate incorporation into cellular or oncogenic Ha-Ras in NIH 3T3 cells. S-nitrosocysteine 0-17 Harvey rat sarcoma virus oncogene Mus musculus 163-169 10801823-4 2000 S-nitrosocysteine (SNC) and, to a more modest extent, S-nitrosoglutathione were found to rapidly increase [(3)H]palmitate incorporation into cellular or oncogenic Ha-Ras in NIH 3T3 cells. S-Nitrosoglutathione 54-74 Harvey rat sarcoma virus oncogene Mus musculus 163-169 10801823-4 2000 S-nitrosocysteine (SNC) and, to a more modest extent, S-nitrosoglutathione were found to rapidly increase [(3)H]palmitate incorporation into cellular or oncogenic Ha-Ras in NIH 3T3 cells. [(3)h]palmitate 106-121 Harvey rat sarcoma virus oncogene Mus musculus 163-169 10801823-6 2000 SNC accelerated loss of [(3)H]palmitate from Ha-Ras, implying that SNC stimulated deacylation and permitted subsequent reacylation of Ha-Ras. [(3)h]palmitate 24-39 Harvey rat sarcoma virus oncogene Mus musculus 45-51 10801823-6 2000 SNC accelerated loss of [(3)H]palmitate from Ha-Ras, implying that SNC stimulated deacylation and permitted subsequent reacylation of Ha-Ras. [(3)h]palmitate 24-39 Harvey rat sarcoma virus oncogene Mus musculus 134-140 10801823-7 2000 SNC also decreased Ha-Ras GTP binding and inhibited phosphorylation of the kinases ERK1 and ERK2 in NIH 3T3 cells. Guanosine Triphosphate 26-29 Harvey rat sarcoma virus oncogene Mus musculus 19-25 10801823-9 2000 These results identify SNC as a new tool for manipulating palmitate turnover on Ha-Ras and for studying requirements of repalmitoylation and the relationship between palmitate cycling, membrane localization, and signaling by Ha-Ras. Palmitates 58-67 Harvey rat sarcoma virus oncogene Mus musculus 80-86 10844550-5 2000 All papillomas analyzed (six of six) had mutations in codon 61 of H-ras, demonstrating strong cooperation between the Nf1 GTPase activating protein for Ras, neurofibromin, and Ras-GTP. Guanosine Triphosphate 122-125 Harvey rat sarcoma virus oncogene Mus musculus 66-71 10860937-4 2000 To test this hypothesis, we evaluated mitogen-activated c-Ha-ras expression in vSMCs treated with BaP or its metabolic intermediates alone, and in combination with agents that modulate cellular redox status. Benzo(a)pyrene 98-101 Harvey rat sarcoma virus oncogene Mus musculus 56-64 10860937-6 2000 Ellipticine (0.01 nM), a known inhibitor of cytochrome P450 metabolism and aryl hydrocarbon receptor (AhR) antagonist, inhibited c-Ha-ras induction by BaP (3 microM). ellipticine 0-11 Harvey rat sarcoma virus oncogene Mus musculus 129-137 10860937-6 2000 Ellipticine (0.01 nM), a known inhibitor of cytochrome P450 metabolism and aryl hydrocarbon receptor (AhR) antagonist, inhibited c-Ha-ras induction by BaP (3 microM). Benzo(a)pyrene 151-154 Harvey rat sarcoma virus oncogene Mus musculus 129-137 10860937-7 2000 Serum challenge of G(0) synchronized cultures of vSMCs with DL-buthionine-(S,R)-sulfoximine (0.1 mM), a depletor of cellular glutathione, increased c-Ha-ras mRNA levels during the early phase of the mitogenic response. vsmcs 49-54 Harvey rat sarcoma virus oncogene Mus musculus 148-156 10860937-7 2000 Serum challenge of G(0) synchronized cultures of vSMCs with DL-buthionine-(S,R)-sulfoximine (0.1 mM), a depletor of cellular glutathione, increased c-Ha-ras mRNA levels during the early phase of the mitogenic response. Buthionine Sulfoximine 60-91 Harvey rat sarcoma virus oncogene Mus musculus 148-156 10860937-8 2000 Combined BaP/DL-buthionine-(S, R)-sulfoximine challenge was cytotoxic to the cells and inhibited c-Ha-ras expression, whereas up-regulation of antioxidant capacity by N-acetylcysteine (0.5 mM) precluded BaP-induced ras expression. Benzo(a)pyrene 9-12 Harvey rat sarcoma virus oncogene Mus musculus 97-105 10860937-8 2000 Combined BaP/DL-buthionine-(S, R)-sulfoximine challenge was cytotoxic to the cells and inhibited c-Ha-ras expression, whereas up-regulation of antioxidant capacity by N-acetylcysteine (0.5 mM) precluded BaP-induced ras expression. buthionine 13-26 Harvey rat sarcoma virus oncogene Mus musculus 97-105 10860937-8 2000 Combined BaP/DL-buthionine-(S, R)-sulfoximine challenge was cytotoxic to the cells and inhibited c-Ha-ras expression, whereas up-regulation of antioxidant capacity by N-acetylcysteine (0.5 mM) precluded BaP-induced ras expression. (s, r)-sulfoximine 27-45 Harvey rat sarcoma virus oncogene Mus musculus 97-105 10871846-5 2000 We showed that H-ras null mutant mice develop approximately six times less papillomas compared with wild-type littermates after 20 weeks of TPA treatment. Tetradecanoylphorbol Acetate 140-143 Harvey rat sarcoma virus oncogene Mus musculus 15-20 10928116-2 2000 In order to determine whether this promising antineoplastic activity would extend to anticarcinogenic properties, the effect of MBB on the 7,12-dimethylbenz [alpha]anthracene (DMBA)-induced expression of the c-myc, Ha-ras and p53 genes in isolated RNA from the liver, lung, kidney, spleen, thymus, lymph nodes and bone marrow of CBA/Ca inbred mice was investigated. 7,12-dimethylbenz [alpha]anthracene 139-174 Harvey rat sarcoma virus oncogene Mus musculus 215-221 10928116-5 2000 The 48-hour experiment with female and male CBA/Ca inbred mice also determined the compound which effectively reduced the DMBA-induced c-myc and Ha-ras overexpressions in almost all tissues. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 122-126 Harvey rat sarcoma virus oncogene Mus musculus 145-151 10779650-0 2000 Carcinogen dose-dependent variation in the transgene mutation spectrum in urethane-induced lung tumors in transgenic mice carrying the human prototype c-Ha-ras gene. Urethane 74-82 Harvey rat sarcoma virus oncogene Mus musculus 151-159 10779650-5 2000 CAG to CTG transversions were observed in the c-Ha-ras gene in these lung proliferative lesions of rasH2 mice of the single injection group at high incidence (male: 58.3%, female: 62.5%), but no mutations of the mouse c-Ki-ras gene were evident in either rasH2 or non-Tg mice. GUANOSINE 5'-TRIPHOSPHATE P3-[1-(2-NITROPHENYL)ETHYL ESTER] 0-3 Harvey rat sarcoma virus oncogene Mus musculus 46-54 10779650-5 2000 CAG to CTG transversions were observed in the c-Ha-ras gene in these lung proliferative lesions of rasH2 mice of the single injection group at high incidence (male: 58.3%, female: 62.5%), but no mutations of the mouse c-Ki-ras gene were evident in either rasH2 or non-Tg mice. ctg 7-10 Harvey rat sarcoma virus oncogene Mus musculus 46-54 10928071-2 2000 In order to further study the kinetics and significance of these gene expression changes, we determined the effect of 1-nitropyrene treatment on the expression of c-myc, p53, Ha-ras, N-ras, and Ki-ras genes, 24 hours after treatment. 1-nitropyrene 118-131 Harvey rat sarcoma virus oncogene Mus musculus 175-181 10904878-0 2000 Deuterium depletion can decrease the expression of C-myc Ha-ras and p53 gene in carcinogen-treated mice. Deuterium 0-9 Harvey rat sarcoma virus oncogene Mus musculus 57-63 10815806-4 2000 Its substitution with glutamic acid results in a catalytically inactive mutant, which is able to efficiently displace wild-type GEF from p21ras and to originate a stable ras/GEF binary complex due to the reduced affinity of the nucleotide-free ras/GEF complex for the incoming nucleotide. Glutamic Acid 22-35 Harvey rat sarcoma virus oncogene Mus musculus 137-143 10729330-6 2000 In the H-ras null mutant hippocampus, the tyrosine phosphorylation of NR2A (epsilon1) and NR2B (epsilon2) subunits of NMDA receptors is increased, and, correspondingly, NMDA synaptic responses are selectively enhanced. Tyrosine 42-50 Harvey rat sarcoma virus oncogene Mus musculus 7-12 10746633-6 2000 Insulin-resistant glucosamine-treated animals displayed significantly increased farnesylated p21Ras in response to insulin infusion compared to that in control saline-treated animals. Glucosamine 18-29 Harvey rat sarcoma virus oncogene Mus musculus 93-99 10729330-6 2000 In the H-ras null mutant hippocampus, the tyrosine phosphorylation of NR2A (epsilon1) and NR2B (epsilon2) subunits of NMDA receptors is increased, and, correspondingly, NMDA synaptic responses are selectively enhanced. N-Methylaspartate 118-122 Harvey rat sarcoma virus oncogene Mus musculus 7-12 10747294-8 2000 Activating Ha-ras mutations were present in essentially all DMBA-initiated tumors and about 70% of MNNG-initiated tumors. 9,10-Dimethyl-1,2-benzanthracene 60-64 Harvey rat sarcoma virus oncogene Mus musculus 11-17 10657963-0 2000 Using polymerase arrest to detect DNA binding specificity of aristolochic acid in the mouse H-ras gene. aristolochic acid I 61-78 Harvey rat sarcoma virus oncogene Mus musculus 92-97 10767618-10 2000 In liver tumors induced by vinyl chloride, specific mutational patterns were found in the Ki-ras gene in human ASL, in the Ha-ras gene in hepatocellular carcinoma (HCC) in rats, and in the p53 gene in human and rat ASL. Vinyl Chloride 27-41 Harvey rat sarcoma virus oncogene Mus musculus 123-129 10767618-11 2000 In tumors induced by urethane in mice, codon 61 of the Ha-ras gene (liver, skin) and of the Ki-ras gene (lung) seems to be a characteristic target. Urethane 21-29 Harvey rat sarcoma virus oncogene Mus musculus 55-61 10791191-7 2000 Tumor growth in antisense H-ras treated animals was significantly retarded in comparison with that of the untreated (t = 3.509, P < 0.01) or non-specific antisense oligodeoxynucleotide treated animals (t = 3.452, P < 0.01). Oligodeoxyribonucleotides 167-187 Harvey rat sarcoma virus oncogene Mus musculus 26-31 10708482-7 2000 Sequence analysis of the endogenous c-Ha-ras from spontaneous and TPA-induced HK1 less than, with dotras/alpha papillomas revealed wild-type sequence. Tetradecanoylphorbol Acetate 66-69 Harvey rat sarcoma virus oncogene Mus musculus 36-44 10657963-5 2000 Polymerase arrest spectra showed a preference for reaction with purine bases in the mouse H-ras gene for both activated compounds, consistent with previous results that purine adducts are the principal reaction products of AAI and AAII with DNA. purine 64-70 Harvey rat sarcoma virus oncogene Mus musculus 90-95 10657963-5 2000 Polymerase arrest spectra showed a preference for reaction with purine bases in the mouse H-ras gene for both activated compounds, consistent with previous results that purine adducts are the principal reaction products of AAI and AAII with DNA. purine 169-175 Harvey rat sarcoma virus oncogene Mus musculus 90-95 10521493-6 1999 Measurement of c-Ha-ras transcription rates and protein expression by nuclear run-on and metabolic labeling assays showed a 5-12-fold enhancement, respectively, following treatment with 17beta-estradiol that was blunted by ICI 182,780 in the nonmetastatic variant. Estradiol 186-202 Harvey rat sarcoma virus oncogene Mus musculus 15-23 10769699-3 2000 In order to determine whether this promising antineoplastic activity would extend to anticarcinogenic properties, the effects of MBB on the 7,12-dimethylbenz[alpha] anthracene (DMBA)-induced expression of c-myc, Ha-ras and p53 genes in isolated RNA from liver, lung, kidney, spleen, thymus, lymph nodes and bone marrow of CBA/Ca inbred mice was investigated. 7,12-dimethylbenz[alpha] anthracene 140-175 Harvey rat sarcoma virus oncogene Mus musculus 212-218 10656492-5 1999 The abnormal expression of Ha-ras in the latter cells may enhance in this system the effects of the BP apoptosis path reported for BP-transformed Hepa 1c1c7 hepatoma cells. Benzo(a)pyrene 100-102 Harvey rat sarcoma virus oncogene Mus musculus 27-33 10656492-5 1999 The abnormal expression of Ha-ras in the latter cells may enhance in this system the effects of the BP apoptosis path reported for BP-transformed Hepa 1c1c7 hepatoma cells. Benzo(a)pyrene 131-133 Harvey rat sarcoma virus oncogene Mus musculus 27-33 10590312-3 1999 We previously demonstrated dislodgment of H-Ras from EJ cell membranes by S-trans,trans-farnesylthiosalicylic acid (FTS), and proposed that FTS disrupts the interactions between the S-prenyl moiety of Ras and the membrane anchorage domains. farnesylthiosalicylic acid 74-114 Harvey rat sarcoma virus oncogene Mus musculus 42-47 10590312-3 1999 We previously demonstrated dislodgment of H-Ras from EJ cell membranes by S-trans,trans-farnesylthiosalicylic acid (FTS), and proposed that FTS disrupts the interactions between the S-prenyl moiety of Ras and the membrane anchorage domains. farnesylthiosalicylic acid 116-119 Harvey rat sarcoma virus oncogene Mus musculus 42-47 10469612-0 1999 Dose-dependent mutation profile in the c-Ha-ras proto-oncogene of skin tumors in mice initiated with benzo[a]pyrene. Benzo(a)pyrene 101-115 Harvey rat sarcoma virus oncogene Mus musculus 39-47 10469612-13 1999 In summary, our data indicate that: (i) 79% of the BP/TPA skin tumors in CD-1 mice had c-Ha-ras mutations for the combined data for high dose and low dose tumors; (ii) the major mutations detected in BP/TPA skin tumors were G-->T transversions; (iii) the global mutation profile in the c-Ha-ras proto-oncogene in skin tumors obtained after initiation with a low dose of BP was different from that obtained after initiation with a high dose of BP. Tetradecanoylphorbol Acetate 54-57 Harvey rat sarcoma virus oncogene Mus musculus 87-95 10469620-8 1999 These results show that melanocytes expressing an activated Ha-ras in the TPras transgenic mice are susceptible to induction of melanoma by DMBA. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 140-144 Harvey rat sarcoma virus oncogene Mus musculus 60-66 10469612-6 1999 The data indicate that c-Ha-ras mutations can be detected in normal-looking and hyperplastic epidermal cells as well as in tumor cells obtained from mice initiated with BP and promoted with TPA. Benzo(a)pyrene 169-171 Harvey rat sarcoma virus oncogene Mus musculus 23-31 10469612-6 1999 The data indicate that c-Ha-ras mutations can be detected in normal-looking and hyperplastic epidermal cells as well as in tumor cells obtained from mice initiated with BP and promoted with TPA. Tetradecanoylphorbol Acetate 190-193 Harvey rat sarcoma virus oncogene Mus musculus 23-31 10469612-13 1999 In summary, our data indicate that: (i) 79% of the BP/TPA skin tumors in CD-1 mice had c-Ha-ras mutations for the combined data for high dose and low dose tumors; (ii) the major mutations detected in BP/TPA skin tumors were G-->T transversions; (iii) the global mutation profile in the c-Ha-ras proto-oncogene in skin tumors obtained after initiation with a low dose of BP was different from that obtained after initiation with a high dose of BP. Benzo(a)pyrene 51-53 Harvey rat sarcoma virus oncogene Mus musculus 87-95 10478850-4 1999 Stimulation of NG108-15 cells with Ang II induced a decrease in GTP-bound p21ras but a sustained increase in the activity of p42mapk and p44mapk as well as neurite outgrowth. Guanosine Triphosphate 64-67 Harvey rat sarcoma virus oncogene Mus musculus 74-80 10464022-5 1999 In experiments in cell culture, the methyl ester prodrugs of these inhibitors are cell permeant and potently inhibit the posttranslational modification of H-Ras protein. methyl ester 36-48 Harvey rat sarcoma virus oncogene Mus musculus 155-160 10467416-3 1999 In this model, initiation, which involves mutation of H-ras induced by DMBA, can be distinguished from promotion induced by TPA, and progression to carcinoma. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 71-75 Harvey rat sarcoma virus oncogene Mus musculus 54-59 10365912-0 1999 Frequent Ha-ras mutations in murine skin and liver tumors induced by 7H-dibenzo[c,g]carbazole. 7H-dibenzo(c,g)carbazole 69-93 Harvey rat sarcoma virus oncogene Mus musculus 9-15 10442636-5 1999 This C17 neuronal cell-induced Nf1+/- increase in proliferation was blocked by MEK inhibition (PD98059), suggesting a p21-ras-dependent effect. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 95-102 Harvey rat sarcoma virus oncogene Mus musculus 118-125 10499288-5 1999 Moreover, it is interesting to note that the Zn/HL2 complex exhibits specific cytotoxic activity against Pam-ras cells (cis-DDP resistant cells which over-express the H-ras oncogene) with an in vitro therapeutic index of 3.26 versus 0.78 for cis-DDP. Zinc 45-47 Harvey rat sarcoma virus oncogene Mus musculus 167-172 10499288-5 1999 Moreover, it is interesting to note that the Zn/HL2 complex exhibits specific cytotoxic activity against Pam-ras cells (cis-DDP resistant cells which over-express the H-ras oncogene) with an in vitro therapeutic index of 3.26 versus 0.78 for cis-DDP. Cisplatin 120-127 Harvey rat sarcoma virus oncogene Mus musculus 167-172 10225445-3 1999 In in vitro studies, lovastatin inhibited the growth of cells transformed with Ha-ras oncogene (Ras-3T3 and HBL100-ras cells) more effectively than control NIH-3T3 and HBL100-neo cells. Lovastatin 21-31 Harvey rat sarcoma virus oncogene Mus musculus 79-85 10225445-7 1999 Our results suggest that lovastatin increases antitumor activity of TNF-alpha against tumor cells transformed with v-Ha-ras oncogene via inhibition of tumor-induced blood-vessel formation. Lovastatin 25-35 Harvey rat sarcoma virus oncogene Mus musculus 117-123 10225445-0 1999 Lovastatin and tumor necrosis factor-alpha exhibit potentiated antitumor effects against Ha-ras-transformed murine tumor via inhibition of tumor-induced angiogenesis. Lovastatin 0-10 Harvey rat sarcoma virus oncogene Mus musculus 89-95 10222151-2 1999 This paper clearly shows that the sensitivity of NIH/3T3 cells to TNF-alpha cytotoxicity positively correlated with the expression level of activated Ha-ras transgene, which was manipulated either positively by isopropyl-beta-d-thiogalactoside (IPTG) induction or negatively by a ribozyme or a dominant negative Ras suppression. Isopropyl Thiogalactoside 211-243 Harvey rat sarcoma virus oncogene Mus musculus 150-156 10222151-2 1999 This paper clearly shows that the sensitivity of NIH/3T3 cells to TNF-alpha cytotoxicity positively correlated with the expression level of activated Ha-ras transgene, which was manipulated either positively by isopropyl-beta-d-thiogalactoside (IPTG) induction or negatively by a ribozyme or a dominant negative Ras suppression. Isopropyl Thiogalactoside 245-249 Harvey rat sarcoma virus oncogene Mus musculus 150-156 10029582-9 1999 8-Br-cAMP, Dibutyryl-cAMP, Ras-Raf-1 pathway inhibitors, and PD98059, a MAPK kinase inhibitor, suppressed proliferation and phosphorylation of MAPK detected by Western blotting with anti-phospho-MAPK antibody, but not adhesion of any type of H-Ras-transfected Baf3 cells, whereas U-73122, a phospholipase C (PLC) inhibitor, suppressed adhesion of these cells completely. 8-Bromo Cyclic Adenosine Monophosphate 0-9 Harvey rat sarcoma virus oncogene Mus musculus 242-247 10376347-0 1999 A cycloplatinated compound of p-isopropylbenzaldehyde thiosemicarbazone and its chloro-bridged derivative induce apoptosis in cis-DDP resistant cells which overexpress the H-ras oncogene. cuminaldehyde 30-53 Harvey rat sarcoma virus oncogene Mus musculus 172-177 10376347-0 1999 A cycloplatinated compound of p-isopropylbenzaldehyde thiosemicarbazone and its chloro-bridged derivative induce apoptosis in cis-DDP resistant cells which overexpress the H-ras oncogene. Thiosemicarbazones 54-71 Harvey rat sarcoma virus oncogene Mus musculus 172-177 10376347-2 1999 We have analyzed the cytotoxic activity of novel cyclometallated complexes of p-isopropylbenzaldehyde thiosemicarbazone (p-is.TSCN) and their dimeric chloro-bridged derivatives in murine keratinocytes transformed by the H-ras oncogene which are resistant to cis-DDP (Pam-ras cells). p-is 78-82 Harvey rat sarcoma virus oncogene Mus musculus 220-225 10376347-2 1999 We have analyzed the cytotoxic activity of novel cyclometallated complexes of p-isopropylbenzaldehyde thiosemicarbazone (p-is.TSCN) and their dimeric chloro-bridged derivatives in murine keratinocytes transformed by the H-ras oncogene which are resistant to cis-DDP (Pam-ras cells). Sodium Hypochlorite 150-156 Harvey rat sarcoma virus oncogene Mus musculus 220-225 10029582-9 1999 8-Br-cAMP, Dibutyryl-cAMP, Ras-Raf-1 pathway inhibitors, and PD98059, a MAPK kinase inhibitor, suppressed proliferation and phosphorylation of MAPK detected by Western blotting with anti-phospho-MAPK antibody, but not adhesion of any type of H-Ras-transfected Baf3 cells, whereas U-73122, a phospholipase C (PLC) inhibitor, suppressed adhesion of these cells completely. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 61-68 Harvey rat sarcoma virus oncogene Mus musculus 242-247 10029582-9 1999 8-Br-cAMP, Dibutyryl-cAMP, Ras-Raf-1 pathway inhibitors, and PD98059, a MAPK kinase inhibitor, suppressed proliferation and phosphorylation of MAPK detected by Western blotting with anti-phospho-MAPK antibody, but not adhesion of any type of H-Ras-transfected Baf3 cells, whereas U-73122, a phospholipase C (PLC) inhibitor, suppressed adhesion of these cells completely. Bucladesine 11-25 Harvey rat sarcoma virus oncogene Mus musculus 242-247 10096783-4 1999 Liver, aorta, and skeletal muscle of ob/ob mice, and mice made obese and hyperinsulinaemic by injection of gold-thioglucose contained greater amounts of farnesylated p21Ras than tissues of their lean normoinsulinaemic counterparts. Aurothioglucose 107-123 Harvey rat sarcoma virus oncogene Mus musculus 166-172 10424403-4 1999 A down-regulation of VDR expression was observed after Ha-ras transformation of HC-11 cells which desensitized the cells to the growth inhibitory effects of vitamin D3. Cholecalciferol 157-167 Harvey rat sarcoma virus oncogene Mus musculus 55-61 9774435-8 1998 Stable transfection of EGFR-negative NR6 and EGFR-positive Swiss3T3 cells with oncogenic (G12V)Ha-Ras demonstrated that only the Ha-Ras-transfected Swiss 3T3 cells possessed constitutive MAPK activity, and this activity was sensitive to PD153035. 4-((3-bromophenyl)amino)-6,7-dimethoxyquinazoline 237-245 Harvey rat sarcoma virus oncogene Mus musculus 129-135 10029404-4 1999 In particular, ras mutations have been well characterized in the mouse skin two-stage carcinogenesis model, and a large body of literature has indicated that initiation with the genotoxic carcinogen 7,12-dimethylbenz[a]anthracene induces a specific point mutation in Ha-ras gene in this model. 7,12-dimethylbenz 199-216 Harvey rat sarcoma virus oncogene Mus musculus 267-273 10029404-4 1999 In particular, ras mutations have been well characterized in the mouse skin two-stage carcinogenesis model, and a large body of literature has indicated that initiation with the genotoxic carcinogen 7,12-dimethylbenz[a]anthracene induces a specific point mutation in Ha-ras gene in this model. anthracene 219-229 Harvey rat sarcoma virus oncogene Mus musculus 267-273 9856997-3 1998 This work studies the influence of the Ras effector c-Raf-1 on the action of guanine nucleotide exchange factors (GEFs) on Ha-Ras in vitro. Guanine Nucleotides 77-95 Harvey rat sarcoma virus oncogene Mus musculus 123-129 9856997-5 1998 Our results show that not only the intrinsic GTP/GTP exchange on Ha-Ras but also the GEF-stimulated exchange is inhibited in a concentration-dependent manner by the RBDs of Raf. Guanosine Triphosphate 45-48 Harvey rat sarcoma virus oncogene Mus musculus 65-71 9810004-8 1998 In a Ha-Ras transgenic mouse model, prophylactic treatment with SCH 66336 delayed tumor onset, reduced the average number of tumors/mouse, and reduced the average tumor weight/animal. lonafarnib 64-73 Harvey rat sarcoma virus oncogene Mus musculus 5-11 9880524-4 1999 We show that the RA-induced differentiation toward PrE is accompanied by a sustained increase in Ras activity and that ectopic expression of oncogenic Ha-Ras is sufficient to induce PrE differentiation. Tretinoin 17-19 Harvey rat sarcoma virus oncogene Mus musculus 151-157 10433077-1 1999 We previously reported that a decreased TCR mediated activity of the GTP-GDP binding p21ras protooncogene is associated with prediabetes in non-obese diabetic (NOD) mice. Guanosine Triphosphate 69-72 Harvey rat sarcoma virus oncogene Mus musculus 85-91 10433077-1 1999 We previously reported that a decreased TCR mediated activity of the GTP-GDP binding p21ras protooncogene is associated with prediabetes in non-obese diabetic (NOD) mice. Guanosine Diphosphate 73-76 Harvey rat sarcoma virus oncogene Mus musculus 85-91 9856997-5 1998 Our results show that not only the intrinsic GTP/GTP exchange on Ha-Ras but also the GEF-stimulated exchange is inhibited in a concentration-dependent manner by the RBDs of Raf. Guanosine Triphosphate 49-52 Harvey rat sarcoma virus oncogene Mus musculus 65-71 9856997-6 1998 Conversely, the scintillation proximity assay, which monitors the effect of GEF on the Ras.Raf complex, showed that the binding of Raf and GEF to Ha-Ras.GTP is mutually exclusive. Guanosine Triphosphate 153-156 Harvey rat sarcoma virus oncogene Mus musculus 146-152 9856997-8 1998 It is noteworthy that under more physiological conditions mimicking the cellular GDP/GTP ratio, Raf enhances the GEF-stimulated GDP/GTP exchange on Ha-Ras, in agreement with the sequestration of Ras.GTP by Raf. Guanosine Diphosphate 81-84 Harvey rat sarcoma virus oncogene Mus musculus 148-154 9856997-8 1998 It is noteworthy that under more physiological conditions mimicking the cellular GDP/GTP ratio, Raf enhances the GEF-stimulated GDP/GTP exchange on Ha-Ras, in agreement with the sequestration of Ras.GTP by Raf. Guanosine Triphosphate 85-88 Harvey rat sarcoma virus oncogene Mus musculus 148-154 9856997-8 1998 It is noteworthy that under more physiological conditions mimicking the cellular GDP/GTP ratio, Raf enhances the GEF-stimulated GDP/GTP exchange on Ha-Ras, in agreement with the sequestration of Ras.GTP by Raf. Guanosine Diphosphate 128-131 Harvey rat sarcoma virus oncogene Mus musculus 148-154 9856997-8 1998 It is noteworthy that under more physiological conditions mimicking the cellular GDP/GTP ratio, Raf enhances the GEF-stimulated GDP/GTP exchange on Ha-Ras, in agreement with the sequestration of Ras.GTP by Raf. Guanosine Triphosphate 132-135 Harvey rat sarcoma virus oncogene Mus musculus 148-154 9856997-8 1998 It is noteworthy that under more physiological conditions mimicking the cellular GDP/GTP ratio, Raf enhances the GEF-stimulated GDP/GTP exchange on Ha-Ras, in agreement with the sequestration of Ras.GTP by Raf. Guanosine Triphosphate 132-135 Harvey rat sarcoma virus oncogene Mus musculus 148-154 9856997-9 1998 Consistent with our results, the GEF-stimulated exchange of Ha-Ras.GTP was also inhibited by another effector of Ras, the RBD (amino acid residues 133-314) of phosphatidylinositol 3-kinase p110alpha. Guanosine Triphosphate 67-70 Harvey rat sarcoma virus oncogene Mus musculus 60-66 9766659-10 1998 Ha-ras gene codon 61 mutations were detected in 7 of 10 stage I SCHF and 13 of 14 stage II SCHF microdissected from histological sections and 7 of 7 of whole papules by mutation-specific PCR analysis. schf 64-68 Harvey rat sarcoma virus oncogene Mus musculus 0-6 9766659-10 1998 Ha-ras gene codon 61 mutations were detected in 7 of 10 stage I SCHF and 13 of 14 stage II SCHF microdissected from histological sections and 7 of 7 of whole papules by mutation-specific PCR analysis. schf 91-95 Harvey rat sarcoma virus oncogene Mus musculus 0-6 9766437-0 1998 Skin tumorigenesis and Ki-ras and Ha-ras mutations in tumors from adult mice exposed in utero to 3"-azido-2",3"-dideoxythymidine. Zidovudine 97-128 Harvey rat sarcoma virus oncogene Mus musculus 34-40 9766437-9 1998 Ha-ras exon I codons 12 and 13 were mutated in 11 of 19 tumors (58%) from mice given AZT and TPA and in one of 15 tumors (7%) from mice given TPA alone (P= 0.004). Zidovudine 85-88 Harvey rat sarcoma virus oncogene Mus musculus 0-6 9766437-9 1998 Ha-ras exon I codons 12 and 13 were mutated in 11 of 19 tumors (58%) from mice given AZT and TPA and in one of 15 tumors (7%) from mice given TPA alone (P= 0.004). Tetradecanoylphorbol Acetate 93-96 Harvey rat sarcoma virus oncogene Mus musculus 0-6 9766437-9 1998 Ha-ras exon I codons 12 and 13 were mutated in 11 of 19 tumors (58%) from mice given AZT and TPA and in one of 15 tumors (7%) from mice given TPA alone (P= 0.004). Tetradecanoylphorbol Acetate 142-145 Harvey rat sarcoma virus oncogene Mus musculus 0-6 9661886-6 1998 Increasing ODC activity also correlated positively with the ability to grow in soft agar in both the H-Ras- and RhoA-expressing cells. Agar 84-88 Harvey rat sarcoma virus oncogene Mus musculus 101-106 9828784-1 1998 AIMS/BACKGROUND: The authors have developed transgenic mouse strains at the Arizona Cancer Center using a tyrosinase promoter to target expression of the mutated T24 Ha-ras gene in melanin producing cells. Melanins 181-188 Harvey rat sarcoma virus oncogene Mus musculus 166-172 9828784-11 1998 CONCLUSION: Pigmented intraocular tumours developed in transgenic strains of mice that express a mutated Ha-ras gene in melanin producing cells. Melanins 120-127 Harvey rat sarcoma virus oncogene Mus musculus 105-111 9888515-2 1998 We also found that stimulation of NG108-15 cells with Ang II induced a rapid decrease in GTP-bound p21ras. Guanosine Triphosphate 89-92 Harvey rat sarcoma virus oncogene Mus musculus 99-105 9704014-0 1998 Defective retrovirus insertion activates c-Ha-ras protooncogene in an MNU-induced rat mammary carcinoma. Methylnitrosourea 70-73 Harvey rat sarcoma virus oncogene Mus musculus 41-49 9704014-3 1998 here we report that the insertion of a defective retrovirus in the -1 intron of rat c-Ha-ras is responsible for the activation of the gene by over 10-fold overexpression in an MNU-induced rat mammary cancer. Methylnitrosourea 176-179 Harvey rat sarcoma virus oncogene Mus musculus 84-92 9661886-7 1998 In stable transfections, coexpression of the ODC dominant negative mutant K69A/C360A with either HRas(61L) or RhoA(63L) both inhibited intracellular ODC activity and caused a reversion of the transformed phenotype, as measured by a dramatic reduction in the ability of these cells to grow in soft agar and form foci on a monolayer. Agar 297-301 Harvey rat sarcoma virus oncogene Mus musculus 97-101 9671400-0 1998 Detection of dibenzo[a,l]pyrene-induced H-ras codon 61 mutant genes in preneoplastic SENCAR mouse skin using a new PCR-RFLP method. dibenzo(a,l)pyrene 13-31 Harvey rat sarcoma virus oncogene Mus musculus 40-45 9715515-3 1998 The mutation patterns of the human and endogenous mouse c-H-ras codon 61 in these proliferative liver lesions were analyzed by DNA amplification using polymerase chain reaction, single-strand conformation polymorphism (PCR-SSCP), and oligonucleotide dot blot hybridization. Oligonucleotides 234-249 Harvey rat sarcoma virus oncogene Mus musculus 56-63 9715515-4 1998 The hepatocellular carcinomas in 4 males and 1 female contained a point mutation in the mouse c-H-ras gene: 3, 1, and 1 carcinomas had a CAA to AAA transversion at the first base of codon 61, a CAA to CTA transversions, and a CAA to CGA transition at the second base of codon 61, respectively. crotonic acid 201-204 Harvey rat sarcoma virus oncogene Mus musculus 94-101 9671400-2 1998 Papillomas induced by the most carcinogenic environmental polycyclic aromatic hydrocarbon (PAH), dibenzo[a,l]pyrene (DB[a,l]P), in SENCAR mouse skin contain a specific H-ras codon 61 (CAA-->CTA) mutation. Polycyclic Aromatic Hydrocarbons 58-89 Harvey rat sarcoma virus oncogene Mus musculus 168-173 9671400-2 1998 Papillomas induced by the most carcinogenic environmental polycyclic aromatic hydrocarbon (PAH), dibenzo[a,l]pyrene (DB[a,l]P), in SENCAR mouse skin contain a specific H-ras codon 61 (CAA-->CTA) mutation. dibenzo(a,l)pyrene 97-115 Harvey rat sarcoma virus oncogene Mus musculus 168-173 9671400-2 1998 Papillomas induced by the most carcinogenic environmental polycyclic aromatic hydrocarbon (PAH), dibenzo[a,l]pyrene (DB[a,l]P), in SENCAR mouse skin contain a specific H-ras codon 61 (CAA-->CTA) mutation. dibenzo(a,l)pyrene 117-125 Harvey rat sarcoma virus oncogene Mus musculus 168-173 9720766-0 1998 Ligation of the alpha2M signalling receptor elevates the levels of p21Ras-GTP in macrophages. Guanosine Triphosphate 74-77 Harvey rat sarcoma virus oncogene Mus musculus 67-73 9667646-3 1998 We demonstrate that overexpression of activated Ha-ras oncogene by exogenous isopropyl-beta-D-thiogalactoside (IPTG) under serum-depleted conditions can stimulate cell apoptosis. Isopropyl Thiogalactoside 77-109 Harvey rat sarcoma virus oncogene Mus musculus 48-54 9667646-3 1998 We demonstrate that overexpression of activated Ha-ras oncogene by exogenous isopropyl-beta-D-thiogalactoside (IPTG) under serum-depleted conditions can stimulate cell apoptosis. Isopropyl Thiogalactoside 111-115 Harvey rat sarcoma virus oncogene Mus musculus 48-54 9667646-6 1998 Cycloheximide blocked the apoptosis of 7-4 cells in a dose-dependent manner, indicating that specific protein regulating apoptosis may be synthesized through Ha-ras overexpression. Cycloheximide 0-13 Harvey rat sarcoma virus oncogene Mus musculus 158-164 9720766-3 1998 Both alpha2M-methylamine (50 pM) and RBF (50 pM) stimulated a 2-3-fold increase in the formation of the p21Ras-GTP complex compared with unstimulated cells. Guanosine Triphosphate 111-114 Harvey rat sarcoma virus oncogene Mus musculus 104-110 9576960-0 1998 Nitric oxide mediates N-methyl-D-aspartate receptor-induced activation of p21ras. Nitric Oxide 0-12 Harvey rat sarcoma virus oncogene Mus musculus 74-80 9496909-6 1998 Immunoprecipitation showed that farnesylamine inhibited farnesylation of p21ras in vivo. farnesylamine 32-45 Harvey rat sarcoma virus oncogene Mus musculus 73-79 9525741-4 1998 The growth factor antagonist, suramin, inhibited the serum-independent proliferation of Ha-ras transformed fibroblasts, but not the serum-independent proliferation of N-ras transformed cells. Suramin 30-37 Harvey rat sarcoma virus oncogene Mus musculus 88-94 9525741-8 1998 A c-N-ras antisense oligonucleotide, which down-regulated c-N-ras protein levels, abrogated the constitutive MAPK activity and serum-independent proliferation of (G12V)Ha-ras transformed cells. Oligonucleotides 20-35 Harvey rat sarcoma virus oncogene Mus musculus 168-174 9365540-3 1997 H-ras transformation downregulated RAR expression and abolished responsiveness to RA. Tretinoin 35-37 Harvey rat sarcoma virus oncogene Mus musculus 0-5 9419414-7 1997 The inhibitory response of CR-1 in HC-11 cells on beta-casein expression after treatment with DIP can be attenuated by B581, a peptidomimetic farnesyltransferase inhibitor that blocks p21ras farnesylation and activation, and by the phosphatidylinositol 3"-kinase (PI3k) inhibitor LY 294002 but not by PD 98059, a MAPK kinase inhibitor that blocks MAPK activation. dip 94-97 Harvey rat sarcoma virus oncogene Mus musculus 184-190 9419414-7 1997 The inhibitory response of CR-1 in HC-11 cells on beta-casein expression after treatment with DIP can be attenuated by B581, a peptidomimetic farnesyltransferase inhibitor that blocks p21ras farnesylation and activation, and by the phosphatidylinositol 3"-kinase (PI3k) inhibitor LY 294002 but not by PD 98059, a MAPK kinase inhibitor that blocks MAPK activation. B 581 119-123 Harvey rat sarcoma virus oncogene Mus musculus 184-190 9415707-0 1997 Identification of benzo[a]pyrene-inducible cis-acting elements within c-Ha-ras transcriptional regulatory sequences. Benzo(a)pyrene 18-32 Harvey rat sarcoma virus oncogene Mus musculus 70-78 9415707-1 1997 Previous studies in this laboratory have demonstrated that transcriptional deregulation of c-Ha-ras expression is associated with the induction and maintenance of proliferative vascular smooth muscle cell (SMC) phenotypes by benzo[a]pyrene (BaP). Benzo(a)pyrene 225-239 Harvey rat sarcoma virus oncogene Mus musculus 91-99 9415707-1 1997 Previous studies in this laboratory have demonstrated that transcriptional deregulation of c-Ha-ras expression is associated with the induction and maintenance of proliferative vascular smooth muscle cell (SMC) phenotypes by benzo[a]pyrene (BaP). Benzo(a)pyrene 241-244 Harvey rat sarcoma virus oncogene Mus musculus 91-99 9415707-2 1997 We examined previously undescribed cis-acting elements within the proximal 5" regulatory region of c-Ha-ras (-550 to +220) for their ability to influence BaP-induced transcription in murine SMCs. Benzo(a)pyrene 154-157 Harvey rat sarcoma virus oncogene Mus musculus 99-107 9415707-5 1997 The use of monoclonal antibodies to the aryl hydrocarbon receptor transcription factor in competition electrophoretic mobility shift assays indicated this protein is specifically induced by BaP to interact at the AHRE within the c-Ha-ras 5" regulatory region. Benzo(a)pyrene 190-193 Harvey rat sarcoma virus oncogene Mus musculus 229-237 9415707-8 1997 These data indicate that c-Ha-ras gene expression is modulated by BaP via a complex mechanism that likely involves interactions among multiple regulatory elements. Benzo(a)pyrene 66-69 Harvey rat sarcoma virus oncogene Mus musculus 25-33 9374526-10 1997 Of several prenylated GTP-binding proteins tested, including Ki-Ras4B, Ha-Ras, RhoB, RhoA, and Rap1B, only Ki-Ras was found to bind significantly to microtubules, and this was in a prenylation-dependent fashion. Guanosine Triphosphate 22-25 Harvey rat sarcoma virus oncogene Mus musculus 71-77 9394675-8 1997 CONCLUSION: Broadened 1H spectra observed after c-erbB-2 or c-Ha-ras transfection suggest changes of plasma membrane viscosity, which may be related to the oncogene expression. Hydrogen 22-24 Harvey rat sarcoma virus oncogene Mus musculus 60-68 9354452-8 1997 Similarly, mouse 10T1/2 cells that are highly transformed and drug resistant due to alterations in H-ras and a mutant oncogenic form of p53 exhibited significant increases in sensitivity to hydroxyurea, PALA, and MTX when transfected with a vector containing the R2 sequence in antisense orientation and compared to cells containing the same vector without the antisense sequence. Methotrexate 213-216 Harvey rat sarcoma virus oncogene Mus musculus 99-104 9395208-12 1997 This is consistent with the previous observation that the proportion of mutations at T:A sites in codon 61 of the H-ras gene was increased in DCA-induced liver tumors in B6C3F1 mice. Dichloroacetic Acid 142-145 Harvey rat sarcoma virus oncogene Mus musculus 114-119 9472707-0 1998 Comparative mouse skin tumorigenicity and induction of Ha-ras mutations by bay region diol epoxides of 5-methylchrysene and 5,6-dimethylchrysene. diol epoxides 86-99 Harvey rat sarcoma virus oncogene Mus musculus 55-61 9472707-0 1998 Comparative mouse skin tumorigenicity and induction of Ha-ras mutations by bay region diol epoxides of 5-methylchrysene and 5,6-dimethylchrysene. 5-methylchrysene 103-119 Harvey rat sarcoma virus oncogene Mus musculus 55-61 9472707-0 1998 Comparative mouse skin tumorigenicity and induction of Ha-ras mutations by bay region diol epoxides of 5-methylchrysene and 5,6-dimethylchrysene. 5,6-dimethylchrysene 124-144 Harvey rat sarcoma virus oncogene Mus musculus 55-61 9472707-6 1998 Tumors induced by 5,6-diMeCDE had a large number of CAA-->CTA mutations in codon 61 of the Ha-ras gene: 50, 55 and 75% of the tumors analysed had this mutation at the 33, 100 and 400 nmol doses. 1,2-Dihydroxy-5,6-dimethyl-3,4-epoxy-1,2,3,4-tetrahydrochrysene 18-29 Harvey rat sarcoma virus oncogene Mus musculus 94-100 9472707-10 1998 The results of this comparative study support the hypothesis that mutations in the Ha-ras gene in mouse skin tumors induced by PAH diol epoxides occur as a result of their direct reaction with the gene. pah diol epoxides 127-144 Harvey rat sarcoma virus oncogene Mus musculus 83-89 9393768-1 1997 The interplay between cyclic AMP (cAMP)-dependent protein kinase A (PKA)- and p21ras-mediated signaling pathways is expected to determine further loss, maintenance, or modulation of differentiation and proliferation of a particular cell. Cyclic AMP 34-38 Harvey rat sarcoma virus oncogene Mus musculus 78-84 9365540-4 1997 Ha-ras-transformed cells were as active as normal NIH-3T3 cells in RA uptake but were unable to degrade it to medium oxidation product, so that, paradoxically, the resistant cells accumulated 20-30-fold as much RA as the sensitive cells. Tretinoin 67-69 Harvey rat sarcoma virus oncogene Mus musculus 0-6 9365540-4 1997 Ha-ras-transformed cells were as active as normal NIH-3T3 cells in RA uptake but were unable to degrade it to medium oxidation product, so that, paradoxically, the resistant cells accumulated 20-30-fold as much RA as the sensitive cells. Tretinoin 211-213 Harvey rat sarcoma virus oncogene Mus musculus 0-6 9349502-1 1997 R-Ras belongs to a family of low molecular weight GTP-binding proteins and exhibits 55% amino acid identity to H-Ras. Guanosine Triphosphate 50-53 Harvey rat sarcoma virus oncogene Mus musculus 111-116 9345273-4 1997 After incubation with [3H]RA, the level of retinoylated protein in NIH 3T3 cells was about 1.5-fold greater than in NIH Ha-ras-3T3 cells. Tritium 23-25 Harvey rat sarcoma virus oncogene Mus musculus 120-126 9294609-1 1997 Our previous reports have shown that two thirds of 4-nitroquinoline-1-oxide (4NQO)-induced murine oral squamous cell carcinomas (SCC) have Hras1 mutations. 4-Nitroquinoline-1-oxide 51-75 Harvey rat sarcoma virus oncogene Mus musculus 139-144 9363994-2 1997 Mutational analysis of the p53 and H-ras genes was performed for 10 pairs of N-butyl-N-(4-hydroxybutyl)nitrosamine-induced invasive mouse urinary bladder carcinomas and metastatic foci. Butylhydroxybutylnitrosamine 77-114 Harvey rat sarcoma virus oncogene Mus musculus 35-40 9316617-6 1997 When p21ras was immunoprecipitated from lovastatin (25 microns)-treated Nb2 cells and mammary gland explants, the unfarnesylated (inactive) form of ras was shown to be redistributed from the cell membrane to the cytosolic compartment of the cell. Lovastatin 40-50 Harvey rat sarcoma virus oncogene Mus musculus 5-11 9294609-1 1997 Our previous reports have shown that two thirds of 4-nitroquinoline-1-oxide (4NQO)-induced murine oral squamous cell carcinomas (SCC) have Hras1 mutations. 4-Nitroquinoline-1-oxide 77-81 Harvey rat sarcoma virus oncogene Mus musculus 139-144 9247294-0 1997 5-methylcytosine is present in the 5" flanking region of Ha-ras in mouse liver and increases with ageing. 5-Methylcytosine 0-16 Harvey rat sarcoma virus oncogene Mus musculus 57-63 9328442-13 1997 Therefore, under these tandem conditions, tumor yields were additive, indicating that there are at least two distinct populations of mutant Ha-ras cells: one promoted by mirex and the other by TPA. Tetradecanoylphorbol Acetate 193-196 Harvey rat sarcoma virus oncogene Mus musculus 140-146 9269990-5 1997 ACNU-induced skin tumors harbored mutations in the c-Ha-ras gene in both groups of mice. Nimustine 0-4 Harvey rat sarcoma virus oncogene Mus musculus 51-59 9296517-2 1997 As shown previously, expression of Ha-ras oncogene in NIH 3T3 fibroblasts (+ ras cells) increases cellular concentrations of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 and enhances bradykinin induced Ca2+ entry [1-3]. Inositol 1,4,5-Trisphosphate 125-137 Harvey rat sarcoma virus oncogene Mus musculus 35-41 9296517-2 1997 As shown previously, expression of Ha-ras oncogene in NIH 3T3 fibroblasts (+ ras cells) increases cellular concentrations of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 and enhances bradykinin induced Ca2+ entry [1-3]. inositol-1,3,4,5-tetrakisphosphate 142-156 Harvey rat sarcoma virus oncogene Mus musculus 35-41 9276648-0 1997 Dichloroacetic acid reduces Ha-ras codon 61 mutations in liver tumors from female B6C3F1 mice. Dichloroacetic Acid 0-19 Harvey rat sarcoma virus oncogene Mus musculus 28-34 9223455-3 1997 Associated with DIGEM, many signal transducer molecules such as c-Src, FAK, and the low-molecular-weight G-proteins Rho A and H-Ras were also found. digem 16-21 Harvey rat sarcoma virus oncogene Mus musculus 126-131 9230198-7 1997 Interestingly, subsequent introduction of an activated c-H-ras oncogene into the c-myc-transfected CHST8 cells resulted not only in escape from the growth arrest at 39 degrees C but also in complete inhibition of the phenobarbital-inducible apoptosis along with de novo induction of the Bax antagonist, Bcl-2. Phenobarbital 217-230 Harvey rat sarcoma virus oncogene Mus musculus 55-62 9230273-11 1997 These results demonstrate that a mismatch containing an incorrect adenine on either strand at the H-ras codon 12 middle base pair location is most likely to undergo a mutational event in NIH 3T3 cells. Adenine 66-73 Harvey rat sarcoma virus oncogene Mus musculus 98-103 9210956-8 1997 Relative to littermates lacking the transgene, the induction of Cyp1a-1 by TCDD was partially suppressed (about 50%) in the epidermises of v-Ha-ras-positive transgenic mice. Polychlorinated Dibenzodioxins 75-79 Harvey rat sarcoma virus oncogene Mus musculus 141-147 9210956-10 1997 induction of Cyp1a-1 by TCDD was also suppressed (more than 98%) in chemically induced skin papillomas having Ha-ras mutations, relative to uninvolved surrounding skin. Polychlorinated Dibenzodioxins 24-28 Harvey rat sarcoma virus oncogene Mus musculus 110-116 9115586-7 1997 Characteristic codon 61 mutations in the Ha-ras gene were found in most of the papillomas and SCCs induced by DMBA and TPA in transgenic as well as nontransgenic mice. 9,10-Dimethyl-1,2-benzanthracene 110-114 Harvey rat sarcoma virus oncogene Mus musculus 41-47 9111215-0 1997 Both K-ras and H-ras protooncogene mutations are associated with Harderian gland tumorigenesis in B6C3F1 mice exposed to isoprene for 26 weeks. isoprene 121-129 Harvey rat sarcoma virus oncogene Mus musculus 15-20 9111215-5 1997 Mutations in K-ras and H-ras were identified by single-strand conformational analysis and direct sequencing of polymerase chain reaction (PCR) amplified DNA, isolated from paraffin-embedded sections of HG neoplasms. Paraffin 172-180 Harvey rat sarcoma virus oncogene Mus musculus 23-28 9111215-7 1997 All of the isoprene-induced HG neoplasms exhibited activated K-ras (60%) or H-ras (40%) mutations. isoprene 11-19 Harvey rat sarcoma virus oncogene Mus musculus 76-81 9111215-8 1997 In contrast, ras mutations were detected in 69% of HG neoplasms from 1,3-butadiene exposed mice (14% K-ras and 55% H-ras) and in 56% of HG neoplasms obtained from control B6C3F1 mice (8% K-ras and 48% H-ras). 1,3-butadiene 69-82 Harvey rat sarcoma virus oncogene Mus musculus 115-120 9111215-9 1997 The predominant mutations in isoprene-induced HG neoplasms, but not in previously or newly analysed 1,3-butadiene-induced HG neoplasms, consisted of A-->T transversions (CAA-->CTA) at K-ras codon 61 (15/30) and C-->A transversions (CAA-->AAA) at H-ras codon 61 (8/30). isoprene 29-37 Harvey rat sarcoma virus oncogene Mus musculus 258-263 9111215-11 1997 Isoprene-induced HG neoplasms with K-ras or H-ras mutations had an elevated proliferating cell nuclear antigen (PCNA) index, compared to spontaneous HG neoplasms without ras mutations. isoprene 0-8 Harvey rat sarcoma virus oncogene Mus musculus 44-49 9084984-0 1997 Activation of Na+/H(+)-exchanger by transforming Ha-ras requires stimulated cellular calcium influx and is associated with rearrangement of the actin cytoskeleton. Calcium 85-92 Harvey rat sarcoma virus oncogene Mus musculus 49-55 9084984-6 1997 It is shown that bepridil blocks both cellular calcium entry as measured by Mn2+ quenching of fura-2 fluorescence and activation of the Na+/H(+)-exchanger following expression of the Ha-ras oncogene. Bepridil 17-25 Harvey rat sarcoma virus oncogene Mus musculus 183-189 9084984-12 1997 The results show that both cytoskeletal rearrangement and activation of the Na+/H(+)-exchanger following expression of the Ha-ras oncogene require stimulated calcium influx and Cai oscillations. Calcium 158-165 Harvey rat sarcoma virus oncogene Mus musculus 123-129 9115586-10 1997 These results demonstrate that TGF alpha, through EGFR overstimulation, can act synergistically with TPA to induce the formation, growth, and development of DMBA-initiated skin tumors containing classic Ha-ras gene mutations but not p53 gene inactivation. 9,10-Dimethyl-1,2-benzanthracene 157-161 Harvey rat sarcoma virus oncogene Mus musculus 203-209 9180923-1 1997 We have previously shown that all CBA/J mice exposed to 4-nitroquinoline-1-oxide (4NQO) eventually develop oral cavity squamous cell carcinomas, and two-thirds of these tumors have Ha-ras-1 (Hras1) point mutations at codon 12. 4-Nitroquinoline-1-oxide 56-80 Harvey rat sarcoma virus oncogene Mus musculus 181-189 9180923-1 1997 We have previously shown that all CBA/J mice exposed to 4-nitroquinoline-1-oxide (4NQO) eventually develop oral cavity squamous cell carcinomas, and two-thirds of these tumors have Ha-ras-1 (Hras1) point mutations at codon 12. 4-Nitroquinoline-1-oxide 56-80 Harvey rat sarcoma virus oncogene Mus musculus 191-196 9180923-1 1997 We have previously shown that all CBA/J mice exposed to 4-nitroquinoline-1-oxide (4NQO) eventually develop oral cavity squamous cell carcinomas, and two-thirds of these tumors have Ha-ras-1 (Hras1) point mutations at codon 12. 4-Nitroquinoline-1-oxide 82-86 Harvey rat sarcoma virus oncogene Mus musculus 191-196 9142214-0 1997 Frequency of Ha-ras-1 gene mutations inversely correlated with furan dose in mouse liver tumors. furan 63-68 Harvey rat sarcoma virus oncogene Mus musculus 13-21 9142214-4 1997 After polymerase chain reaction amplification of isolated DNA, slot-blot oligonucleotide hybridization was used to screen for mutations at known mutational hot-spots in the first three exons of Ha-ras-1 (Hras1). Oligonucleotides 73-88 Harvey rat sarcoma virus oncogene Mus musculus 194-202 9142214-4 1997 After polymerase chain reaction amplification of isolated DNA, slot-blot oligonucleotide hybridization was used to screen for mutations at known mutational hot-spots in the first three exons of Ha-ras-1 (Hras1). Oligonucleotides 73-88 Harvey rat sarcoma virus oncogene Mus musculus 204-209 9142214-6 1997 The lack of histomorphologic evidence of chronic cytotoxicity in the livers and the pattern of Hras1 activation suggest that a genotoxic mode of action is responsible for at least part of the hepatocarcinogenicity of furan in B6C3F1 mice. furan 217-222 Harvey rat sarcoma virus oncogene Mus musculus 95-100 9115586-7 1997 Characteristic codon 61 mutations in the Ha-ras gene were found in most of the papillomas and SCCs induced by DMBA and TPA in transgenic as well as nontransgenic mice. Tetradecanoylphorbol Acetate 119-122 Harvey rat sarcoma virus oncogene Mus musculus 41-47 9330640-9 1997 Cells transformed with H-ras manifest an increase in methotrexate resistance as measured by an increase in Dhfr gene amplification. Methotrexate 53-65 Harvey rat sarcoma virus oncogene Mus musculus 23-28 9078246-0 1997 cAMP suppresses p21ras and Raf-1 responses but not the Erk-1 response to granulocyte-colony-stimulating factor: possible Raf-1-independent activation of Erk-1. Cyclic AMP 0-4 Harvey rat sarcoma virus oncogene Mus musculus 16-22 9013950-9 1997 Thus, Shc tyrosine phosphorylation may be the primary CD45-dependent mechanism by which Ag receptors are coupled to the p21ras pathway in J558Lmicrom3. Tyrosine 10-18 Harvey rat sarcoma virus oncogene Mus musculus 120-126 9049185-9 1997 Increased expression of Ha-ras was evident in some spontaneous B6C3F1 liver tumors and in most of the PB-induced liver tumors. Phenobarbital 102-104 Harvey rat sarcoma virus oncogene Mus musculus 24-30 9013950-7 1997 Analysis of specific effector molecules revealed that tyrosine phosphorylation of Shc, but not rasGAP or Vav, correlated with the unique ability of BCR ligation to trigger p21ras activation in CD45+ cells. Tyrosine 54-62 Harvey rat sarcoma virus oncogene Mus musculus 172-178 9022041-2 1997 When grown in medium containing more than 0.1 mM isopropyl beta-D-thiogalactoside (IPTG), the Ha-ras gene was induced up to 20-fold. Isopropyl Thiogalactoside 49-81 Harvey rat sarcoma virus oncogene Mus musculus 94-100 9022041-2 1997 When grown in medium containing more than 0.1 mM isopropyl beta-D-thiogalactoside (IPTG), the Ha-ras gene was induced up to 20-fold. Isopropyl Thiogalactoside 83-87 Harvey rat sarcoma virus oncogene Mus musculus 94-100 9022041-5 1997 Ha-ras gene expression in tumors was strongly induced in the presence of IPTG in vivo. Isopropyl Thiogalactoside 73-77 Harvey rat sarcoma virus oncogene Mus musculus 0-6 9022041-6 1997 Induction of Ha-ras gene expression in mice was consistently observed after 48 hr of exposure to drinking water containing IPTG. Water 106-111 Harvey rat sarcoma virus oncogene Mus musculus 13-19 9022041-6 1997 Induction of Ha-ras gene expression in mice was consistently observed after 48 hr of exposure to drinking water containing IPTG. Isopropyl Thiogalactoside 123-127 Harvey rat sarcoma virus oncogene Mus musculus 13-19 9006088-0 1996 Quantitation of early clonal expansion of two mutant 61st codon c-Ha-ras alleles in DMBA/TPA treated mouse skin by nested PCR/RFLP. 9,10-Dimethyl-1,2-benzanthracene 84-88 Harvey rat sarcoma virus oncogene Mus musculus 64-72 9030247-2 1997 A murine NIH/3T3-derived cell line, designated 2-12, contains an inducible Ha-ras oncogene, which is regulated by the Escherichia coli (E. coli) lac operator/repressor system, and displays a transformed phenotype after isopropyl-beta-D-thiogalactoside induction. Lactose 145-148 Harvey rat sarcoma virus oncogene Mus musculus 75-81 9030247-2 1997 A murine NIH/3T3-derived cell line, designated 2-12, contains an inducible Ha-ras oncogene, which is regulated by the Escherichia coli (E. coli) lac operator/repressor system, and displays a transformed phenotype after isopropyl-beta-D-thiogalactoside induction. Isopropyl Thiogalactoside 219-251 Harvey rat sarcoma virus oncogene Mus musculus 75-81 9006088-0 1996 Quantitation of early clonal expansion of two mutant 61st codon c-Ha-ras alleles in DMBA/TPA treated mouse skin by nested PCR/RFLP. Tetradecanoylphorbol Acetate 89-92 Harvey rat sarcoma virus oncogene Mus musculus 64-72 8954966-7 1996 In fact, inhibition of p21(ras) by transfecting E32-4-2 cells with the transdominant negative mutant of H-ras (L61, S186) led, analogously to lovastatin or (alpha-hydroxyfarnesyl) phosphonic acid treatment, to a strong increase of stress-induced ROS levels. Lovastatin 142-152 Harvey rat sarcoma virus oncogene Mus musculus 104-109 8954966-7 1996 In fact, inhibition of p21(ras) by transfecting E32-4-2 cells with the transdominant negative mutant of H-ras (L61, S186) led, analogously to lovastatin or (alpha-hydroxyfarnesyl) phosphonic acid treatment, to a strong increase of stress-induced ROS levels. (alpha-hydroxyfarnesyl)phosphonic acid 156-195 Harvey rat sarcoma virus oncogene Mus musculus 104-109 8954966-7 1996 In fact, inhibition of p21(ras) by transfecting E32-4-2 cells with the transdominant negative mutant of H-ras (L61, S186) led, analogously to lovastatin or (alpha-hydroxyfarnesyl) phosphonic acid treatment, to a strong increase of stress-induced ROS levels. Reactive Oxygen Species 246-249 Harvey rat sarcoma virus oncogene Mus musculus 104-109 8977326-4 1996 Here we demonstrate that phorbol ester phorbol 12-myrislate 13-acetate (PMA), which increases tyrosine phosphorylation by stimulating protein kinase C and p21ras, can overcome the CTLA-4-mediated inhibition of T cell proliferation. Phorbol Esters 25-38 Harvey rat sarcoma virus oncogene Mus musculus 155-161 8951239-0 1996 Using UvrABC nuclease to detect 7,12-dimethylbenz[a]anthracene anti-diol epoxide-DNA binding specificity in the mouse H-ras gene. 7,12-dimethylbenz[a 32-51 Harvey rat sarcoma virus oncogene Mus musculus 118-123 8951239-0 1996 Using UvrABC nuclease to detect 7,12-dimethylbenz[a]anthracene anti-diol epoxide-DNA binding specificity in the mouse H-ras gene. anthracene 52-62 Harvey rat sarcoma virus oncogene Mus musculus 118-123 8951239-0 1996 Using UvrABC nuclease to detect 7,12-dimethylbenz[a]anthracene anti-diol epoxide-DNA binding specificity in the mouse H-ras gene. diol epoxide 68-80 Harvey rat sarcoma virus oncogene Mus musculus 118-123 8951239-4 1996 This method was used to analyze the anti-DMBADE-DNA binding spectrum in the exon 2 region of the mouse H-ras gene, and it was found that anti-DMBADE binds to the two adenine residues at codon 61 of the H-ras gene with an average affinity. dmbade 41-47 Harvey rat sarcoma virus oncogene Mus musculus 103-108 8951239-4 1996 This method was used to analyze the anti-DMBADE-DNA binding spectrum in the exon 2 region of the mouse H-ras gene, and it was found that anti-DMBADE binds to the two adenine residues at codon 61 of the H-ras gene with an average affinity. dmbade 41-47 Harvey rat sarcoma virus oncogene Mus musculus 202-207 8951239-4 1996 This method was used to analyze the anti-DMBADE-DNA binding spectrum in the exon 2 region of the mouse H-ras gene, and it was found that anti-DMBADE binds to the two adenine residues at codon 61 of the H-ras gene with an average affinity. dmbade 142-148 Harvey rat sarcoma virus oncogene Mus musculus 103-108 8951239-4 1996 This method was used to analyze the anti-DMBADE-DNA binding spectrum in the exon 2 region of the mouse H-ras gene, and it was found that anti-DMBADE binds to the two adenine residues at codon 61 of the H-ras gene with an average affinity. dmbade 142-148 Harvey rat sarcoma virus oncogene Mus musculus 202-207 8951239-4 1996 This method was used to analyze the anti-DMBADE-DNA binding spectrum in the exon 2 region of the mouse H-ras gene, and it was found that anti-DMBADE binds to the two adenine residues at codon 61 of the H-ras gene with an average affinity. Adenine 166-173 Harvey rat sarcoma virus oncogene Mus musculus 103-108 8951239-4 1996 This method was used to analyze the anti-DMBADE-DNA binding spectrum in the exon 2 region of the mouse H-ras gene, and it was found that anti-DMBADE binds to the two adenine residues at codon 61 of the H-ras gene with an average affinity. Adenine 166-173 Harvey rat sarcoma virus oncogene Mus musculus 202-207 8951239-5 1996 Previously, we have demonstrated that syn-DMBADE binds strongly to the adenines at codon 61 of H-ras; these results together suggest that the oncogenic mutation in H-ras may be induced by anti- and syn-DMBADE-DNA adducts. Adenine 71-79 Harvey rat sarcoma virus oncogene Mus musculus 95-100 8951239-5 1996 Previously, we have demonstrated that syn-DMBADE binds strongly to the adenines at codon 61 of H-ras; these results together suggest that the oncogenic mutation in H-ras may be induced by anti- and syn-DMBADE-DNA adducts. Adenine 71-79 Harvey rat sarcoma virus oncogene Mus musculus 164-169 8951239-5 1996 Previously, we have demonstrated that syn-DMBADE binds strongly to the adenines at codon 61 of H-ras; these results together suggest that the oncogenic mutation in H-ras may be induced by anti- and syn-DMBADE-DNA adducts. dmbade 42-48 Harvey rat sarcoma virus oncogene Mus musculus 95-100 8951239-5 1996 Previously, we have demonstrated that syn-DMBADE binds strongly to the adenines at codon 61 of H-ras; these results together suggest that the oncogenic mutation in H-ras may be induced by anti- and syn-DMBADE-DNA adducts. dmbade 42-48 Harvey rat sarcoma virus oncogene Mus musculus 164-169 9007143-2 1996 The compounds examined included: lovastatin, an inhibitor of HMG-CoA reductase, which interferes with membrane localization of p21 ras protein; H-7, a classic inhibitor of protein kinase C; and tiazofurin, a GTP depleting agent, that might affect the GTP/GDP ratio on p21ras. Lovastatin 33-43 Harvey rat sarcoma virus oncogene Mus musculus 127-134 9006114-0 1996 Transplacental mutagenicity of cisplatin: H-ras codon 12 and 13 mutations in skin tumors of SENCAR mice. Cisplatin 31-40 Harvey rat sarcoma virus oncogene Mus musculus 42-47 8977326-4 1996 Here we demonstrate that phorbol ester phorbol 12-myrislate 13-acetate (PMA), which increases tyrosine phosphorylation by stimulating protein kinase C and p21ras, can overcome the CTLA-4-mediated inhibition of T cell proliferation. phorbol 12-myrislate 39-59 Harvey rat sarcoma virus oncogene Mus musculus 155-161 8977326-4 1996 Here we demonstrate that phorbol ester phorbol 12-myrislate 13-acetate (PMA), which increases tyrosine phosphorylation by stimulating protein kinase C and p21ras, can overcome the CTLA-4-mediated inhibition of T cell proliferation. 13-acetate 60-70 Harvey rat sarcoma virus oncogene Mus musculus 155-161 8977326-4 1996 Here we demonstrate that phorbol ester phorbol 12-myrislate 13-acetate (PMA), which increases tyrosine phosphorylation by stimulating protein kinase C and p21ras, can overcome the CTLA-4-mediated inhibition of T cell proliferation. Tetradecanoylphorbol Acetate 72-75 Harvey rat sarcoma virus oncogene Mus musculus 155-161 8977326-4 1996 Here we demonstrate that phorbol ester phorbol 12-myrislate 13-acetate (PMA), which increases tyrosine phosphorylation by stimulating protein kinase C and p21ras, can overcome the CTLA-4-mediated inhibition of T cell proliferation. Tyrosine 94-102 Harvey rat sarcoma virus oncogene Mus musculus 155-161 8950978-3 1996 The contribution of v-Ha-ras to the overall p21ras-GTP content was evaluated by metabolic labelling with 32P. Guanosine Triphosphate 51-54 Harvey rat sarcoma virus oncogene Mus musculus 44-50 8950978-4 1996 Surprisingly, p21ras-GTP complexes were predominant in the serum-deprived BP-A31 cells as well as in the Ras2 cells. Guanosine Triphosphate 21-24 Harvey rat sarcoma virus oncogene Mus musculus 14-20 8950978-5 1996 The excess of p21ras-GTP was not due to the lack of the GTPase activating protein. Guanosine Triphosphate 21-24 Harvey rat sarcoma virus oncogene Mus musculus 14-20 8908192-3 1996 DT cells incubated in the presence of 1 microM dexamethasone (DEX) for 24 hr expressed a relatively high level of membrane-bound Ha-Ras protein, BK B2 receptor mRNA, and B2 receptor binding as determined by Western blotting with anti-Ha-Ras antibodies, reverse transcriptase-polymerase chain reaction using B2 receptor-specific primers, and specific [3H]BK binding, respectively. Dexamethasone 47-60 Harvey rat sarcoma virus oncogene Mus musculus 129-135 8908192-3 1996 DT cells incubated in the presence of 1 microM dexamethasone (DEX) for 24 hr expressed a relatively high level of membrane-bound Ha-Ras protein, BK B2 receptor mRNA, and B2 receptor binding as determined by Western blotting with anti-Ha-Ras antibodies, reverse transcriptase-polymerase chain reaction using B2 receptor-specific primers, and specific [3H]BK binding, respectively. Dexamethasone 47-60 Harvey rat sarcoma virus oncogene Mus musculus 234-240 8908192-3 1996 DT cells incubated in the presence of 1 microM dexamethasone (DEX) for 24 hr expressed a relatively high level of membrane-bound Ha-Ras protein, BK B2 receptor mRNA, and B2 receptor binding as determined by Western blotting with anti-Ha-Ras antibodies, reverse transcriptase-polymerase chain reaction using B2 receptor-specific primers, and specific [3H]BK binding, respectively. Dexamethasone 62-65 Harvey rat sarcoma virus oncogene Mus musculus 129-135 8908192-3 1996 DT cells incubated in the presence of 1 microM dexamethasone (DEX) for 24 hr expressed a relatively high level of membrane-bound Ha-Ras protein, BK B2 receptor mRNA, and B2 receptor binding as determined by Western blotting with anti-Ha-Ras antibodies, reverse transcriptase-polymerase chain reaction using B2 receptor-specific primers, and specific [3H]BK binding, respectively. Dexamethasone 62-65 Harvey rat sarcoma virus oncogene Mus musculus 234-240 8908192-3 1996 DT cells incubated in the presence of 1 microM dexamethasone (DEX) for 24 hr expressed a relatively high level of membrane-bound Ha-Ras protein, BK B2 receptor mRNA, and B2 receptor binding as determined by Western blotting with anti-Ha-Ras antibodies, reverse transcriptase-polymerase chain reaction using B2 receptor-specific primers, and specific [3H]BK binding, respectively. Tritium 351-353 Harvey rat sarcoma virus oncogene Mus musculus 129-135 8908192-5 1996 In the absence of DEX, the DT cells expressed a considerably lower but yet clearly significant level of Ha-Ras. Thymidine 27-29 Harvey rat sarcoma virus oncogene Mus musculus 104-110 9181055-0 1996 Ha-ras oncogene transformation abolishes retinoic acid-induced reduction of intracellular fibronectin. Tretinoin 41-54 Harvey rat sarcoma virus oncogene Mus musculus 0-6 8782662-11 1996 Furthermore, NB-506 decreased the amount of the GTP-bound form of the H-ras product in pT22-3 cells. Guanosine Triphosphate 48-51 Harvey rat sarcoma virus oncogene Mus musculus 70-75 9181055-6 1996 Transformation of NIH-3T3 cells with an activated Ha-ras oncogene downmodulated RAR expression and also abolished responsiveness to RA. Tretinoin 80-82 Harvey rat sarcoma virus oncogene Mus musculus 50-56 9181055-7 1996 A variety of approaches permitted the following conclusions: 1) RA-dependent FN downmodulation is mediated by RARs, 2) retinoid X receptors (RXRs) mediate the observed reduction of RAR alpha by RA, and 3) the blockade of RA responsiveness by Ha-ras-transfected cells cannot be overcome by overexpression of RAR alpha. Tretinoin 64-66 Harvey rat sarcoma virus oncogene Mus musculus 242-248 8876671-0 1996 Protection against malignant conversion in SENCAR mouse skin by all trans retinoic acid: inhibition of the ras p21-processing enzyme farnesyltransferase and Ha-ras p21 membrane localization. Tretinoin 74-87 Harvey rat sarcoma virus oncogene Mus musculus 157-163 8876671-8 1996 Furthermore, the tissue samples from RA-treated groups in different protocols also showed significantly diminished membrane localization of Ha-ras p21, with a concomitant increase in cytosolic Ha-ras p21 levels. Tretinoin 37-39 Harvey rat sarcoma virus oncogene Mus musculus 140-146 8876671-8 1996 Furthermore, the tissue samples from RA-treated groups in different protocols also showed significantly diminished membrane localization of Ha-ras p21, with a concomitant increase in cytosolic Ha-ras p21 levels. Tretinoin 37-39 Harvey rat sarcoma virus oncogene Mus musculus 193-199 8799883-5 1996 The 3,5-di(2,3-epoxypropoxy)xanthone also showed potent inhibitory activity against 212 cells, a Ha-ras oncogene-transformed NIH 3T3 cell line. 3,5-di(2,3-epoxypropoxy)xanthone 4-36 Harvey rat sarcoma virus oncogene Mus musculus 97-103 9173082-3 1996 The normal (Gly(12)) or the transforming (Val(12)) c-H-ras gene was expressed in NIH3T3 cells using a metallothionein promoter. Glycine 12-15 Harvey rat sarcoma virus oncogene Mus musculus 51-58 8753815-0 1996 Novel anti-carcinogenic activity of an organosulfide from garlic: inhibition of H-RAS oncogene transformed tumor growth in vivo by diallyl disulfide is associated with inhibition of p21H-ras processing. organosulfide 39-52 Harvey rat sarcoma virus oncogene Mus musculus 80-85 8753815-0 1996 Novel anti-carcinogenic activity of an organosulfide from garlic: inhibition of H-RAS oncogene transformed tumor growth in vivo by diallyl disulfide is associated with inhibition of p21H-ras processing. diallyl disulfide 131-148 Harvey rat sarcoma virus oncogene Mus musculus 80-85 8753815-2 1996 DADS treatment significantly inhibited the growth of H-ras oncogene transformed tumors in nude mice. diallyl disulfide 0-4 Harvey rat sarcoma virus oncogene Mus musculus 53-58 8753815-8 1996 These results indicate that DADS suppresses the growth of H-ras oncogene transformed tumors in nude mice by inhibiting the membrane association of tumoral p21H-ras. diallyl disulfide 28-32 Harvey rat sarcoma virus oncogene Mus musculus 58-63 8755741-0 1996 Sequence preference of 7,12-dimethylbenz[a]anthracene-syn-diol epoxide-DNA binding in the mouse H-ras gene detected by UvrABC nucleases. 7,12-dimethylbenz 23-40 Harvey rat sarcoma virus oncogene Mus musculus 96-101 8755741-6 1996 We have found that both guanine and adenine residues in codons 12, 13, and 61 of the H-ras gene are strong syn-DMBADE binding sites. Guanine 24-31 Harvey rat sarcoma virus oncogene Mus musculus 85-90 8755741-6 1996 We have found that both guanine and adenine residues in codons 12, 13, and 61 of the H-ras gene are strong syn-DMBADE binding sites. Adenine 36-43 Harvey rat sarcoma virus oncogene Mus musculus 85-90 8755741-6 1996 We have found that both guanine and adenine residues in codons 12, 13, and 61 of the H-ras gene are strong syn-DMBADE binding sites. dmbade 111-117 Harvey rat sarcoma virus oncogene Mus musculus 85-90 8755741-7 1996 These results suggest that the initial binding of DMBADE may greatly contribute to the frequency of H-ras mutations. dmbade 50-56 Harvey rat sarcoma virus oncogene Mus musculus 100-105 21594424-1 1996 We reported recently that azatyrosine inhibits the growth of c-Ha-ras, c-raf or c-erbB-2-transformed NIH3T3 cells and converts the transformed cells to cells with a normal phenotype. beta-(5-hydroxy-2-pyridyl)alanine 26-37 Harvey rat sarcoma virus oncogene Mus musculus 61-69 8681456-0 1996 Activation of H-ras oncogenes in male B6C3F1 mouse liver tumors induced by vinthionine or 2-chloroethyl-methyl sulfide. vinthionine 75-86 Harvey rat sarcoma virus oncogene Mus musculus 14-19 8681456-0 1996 Activation of H-ras oncogenes in male B6C3F1 mouse liver tumors induced by vinthionine or 2-chloroethyl-methyl sulfide. 2-chloroethyl methyl sulfide 90-118 Harvey rat sarcoma virus oncogene Mus musculus 14-19 8681456-9 1996 Thirty seven of 37 vinthionine-induced hepatomas had H-ras mutations in this codon, which consisted of seven C-->A transversions in the first base, with 29 A-->T transversions and one A-->G transition in the second base. vinthionine 19-30 Harvey rat sarcoma virus oncogene Mus musculus 53-58 8621713-4 1996 Antibodies against p21ras, Galphaq, -beta, or -gamma2 subunits of the GTP-binding protein inhibit MAP kinase-dependent phosphorylation. Guanosine Triphosphate 70-73 Harvey rat sarcoma virus oncogene Mus musculus 19-25 8603364-9 1996 This is in contrast to MNU which causes a G to A transition mutation in the 12th codon of the c-Ha-ras proto-oncogene in about one of five mammary cancers induced in pituitary-isografted mice. Methylnitrosourea 23-26 Harvey rat sarcoma virus oncogene Mus musculus 94-102 8622882-7 1996 Gel shift assays demonstrated the mobility pattern of TPA-responsive element (TRE) binding complex with AP-1 derived from H-ras transfectants migrated faster than those from Balb-Neo1, v-myc and H-ras/v-myc. Tetradecanoylphorbol Acetate 54-57 Harvey rat sarcoma virus oncogene Mus musculus 122-127 8622882-7 1996 Gel shift assays demonstrated the mobility pattern of TPA-responsive element (TRE) binding complex with AP-1 derived from H-ras transfectants migrated faster than those from Balb-Neo1, v-myc and H-ras/v-myc. Tetradecanoylphorbol Acetate 54-57 Harvey rat sarcoma virus oncogene Mus musculus 195-200 8625275-0 1996 Hormone dependent and independent mammary tumor development form N-methyl-N-nitrosourea-treated rat mammary epithelial cell xenografts in the nude mouse: multiple pathways and H-ras activation. Methylnitrosourea 65-87 Harvey rat sarcoma virus oncogene Mus musculus 176-181 8626453-2 1996 All-trans-retinoic acid (RA) markedly reduced the level of intracellular fibronectin (FN) in a time- and concentration-dependent fashion in NIH-3T3 cells, but not in NIH-3T3 cells transformed by an activated Ha-ras oncogene. Tretinoin 0-23 Harvey rat sarcoma virus oncogene Mus musculus 208-214 8626453-2 1996 All-trans-retinoic acid (RA) markedly reduced the level of intracellular fibronectin (FN) in a time- and concentration-dependent fashion in NIH-3T3 cells, but not in NIH-3T3 cells transformed by an activated Ha-ras oncogene. Tretinoin 25-27 Harvey rat sarcoma virus oncogene Mus musculus 208-214 8626453-7 1996 Transformation of NIH-3T3 cells with an activated Ha-ras oncogene down-modulated RAR expression and abolished responsiveness to RA. Tretinoin 81-83 Harvey rat sarcoma virus oncogene Mus musculus 50-56 8649795-7 1996 In addition, TAM-67ER inhibited activated c-Ha-ras- or c-raf-induced transformation of NIH3T3 cells. tam-67er 13-21 Harvey rat sarcoma virus oncogene Mus musculus 42-51 8575075-1 1996 Transformation of fibroblast-like cells (NIH 3T3) by a constitutively activated GTP-bound isoform of p21ras (EJ-Ras) produces morphogenic changes characterized by decreased attachment to the substratum, with retraction and rounding of the cell body. Guanosine Triphosphate 80-83 Harvey rat sarcoma virus oncogene Mus musculus 101-107 7495305-9 1995 In addition, the mutant c-H-ras, but not c-myc, cooperated with the functional T antigen at 33 degrees C to allow growth in soft agarose of the CHST8 and CHST10-2.1 cell lines. Sepharose 129-136 Harvey rat sarcoma virus oncogene Mus musculus 24-31 8765412-7 1996 Moreover, we initially demonstrated that IL-12 was effective in preventing and inhibiting the growth of primary tumors induced by the chemical carcinogen methylnitrosourea using c-Ha-ras transgeneic mice. Methylnitrosourea 154-171 Harvey rat sarcoma virus oncogene Mus musculus 178-186 8681026-6 1996 As phosphorylation on tyrosine residues of Lyn, Fyn, and Blk protein tyrosine kinases occurred upon BCR stimulation, these PTKs may be candidates being involved in an induction of not only tyrosine phosphorylation of substrates but also p 21ras activation. Tyrosine 22-30 Harvey rat sarcoma virus oncogene Mus musculus 237-244 8681026-6 1996 As phosphorylation on tyrosine residues of Lyn, Fyn, and Blk protein tyrosine kinases occurred upon BCR stimulation, these PTKs may be candidates being involved in an induction of not only tyrosine phosphorylation of substrates but also p 21ras activation. Tyrosine 69-77 Harvey rat sarcoma virus oncogene Mus musculus 237-244 7591272-2 1995 Rat-6 (R6) embryo fibroblasts transformed by v-myc, rc-mos (rearranged mouse c-mos), v-src and activated c-H-ras oncogenes displayed differential sensitivities to cDDP, and the IC50 values of cDDP decreased by approximately 3.0-, 1.2-, 2.0- and 1.5-fold, respectively. Cisplatin 163-167 Harvey rat sarcoma virus oncogene Mus musculus 105-112 7591272-2 1995 Rat-6 (R6) embryo fibroblasts transformed by v-myc, rc-mos (rearranged mouse c-mos), v-src and activated c-H-ras oncogenes displayed differential sensitivities to cDDP, and the IC50 values of cDDP decreased by approximately 3.0-, 1.2-, 2.0- and 1.5-fold, respectively. Cisplatin 192-196 Harvey rat sarcoma virus oncogene Mus musculus 105-112 7585519-0 1995 The H-ras oncogene interferes with retinoic acid signaling and metabolism in NIH3T3 cells. Tretinoin 35-48 Harvey rat sarcoma virus oncogene Mus musculus 4-9 7487093-1 1995 The effects of expression of the H-ras oncogene on phosphatidylcholine metabolism were examined in C3H10T1/2 and NIH3T3 cells expressing ras constitutively or under the control of inducible promoters. Phosphatidylcholines 51-70 Harvey rat sarcoma virus oncogene Mus musculus 33-38 7592920-4 1995 FTI-277, the methyl ester derivative of FTI-276, was extremely potent (IC50 = 100 nM) at inhibiting H-Ras, but not the geranylgeranylated Rap1A processing in whole cells. methyl ester 13-25 Harvey rat sarcoma virus oncogene Mus musculus 100-105 7592920-6 1995 FTI-277 induced accumulation of cytoplasmic non-farnesylated H-Ras that was able to bind Raf and form cytoplasmic Ras/Raf complexes in which Raf kinase was not activated. FTI 277 0-7 Harvey rat sarcoma virus oncogene Mus musculus 61-66 7585519-3 1995 HPLC analysis of the media and cell extracts demonstrated that H-ras-transformed cells metabolize RA to a much lesser extent than control cells, resulting in a higher concentration of RA in H-ras cells. Tretinoin 98-100 Harvey rat sarcoma virus oncogene Mus musculus 63-68 7585559-6 1995 Our results indicate that SCH 51344 inhibits the ability of v-abl, v-mos, H-ras, v-raf, and mutant active MAP kinase kinase-transformed NIH 3T3 cells to grow in soft agar. SCH 51344 26-35 Harvey rat sarcoma virus oncogene Mus musculus 74-79 7585519-3 1995 HPLC analysis of the media and cell extracts demonstrated that H-ras-transformed cells metabolize RA to a much lesser extent than control cells, resulting in a higher concentration of RA in H-ras cells. Tretinoin 98-100 Harvey rat sarcoma virus oncogene Mus musculus 190-195 7585519-3 1995 HPLC analysis of the media and cell extracts demonstrated that H-ras-transformed cells metabolize RA to a much lesser extent than control cells, resulting in a higher concentration of RA in H-ras cells. Tretinoin 184-186 Harvey rat sarcoma virus oncogene Mus musculus 63-68 7585519-3 1995 HPLC analysis of the media and cell extracts demonstrated that H-ras-transformed cells metabolize RA to a much lesser extent than control cells, resulting in a higher concentration of RA in H-ras cells. Tretinoin 184-186 Harvey rat sarcoma virus oncogene Mus musculus 190-195 7585519-4 1995 Although inactive in endogenous transglutaminase induction, H-ras cell-associated RA was shown to be biologically available to induce activation of a reporter construct containing a retinoid response element and in stimulating transglutaminase activity in nontransfected cells. Retinoids 182-190 Harvey rat sarcoma virus oncogene Mus musculus 60-65 7585519-6 1995 These data demonstrate that, even though H-ras-transformed cells accumulate up to 20 fold the concentration of RA as NIH-3T3 cells, they fail to show transglutaminase induction, suggesting that H-ras interferes with signal transduction by RA. Tretinoin 111-113 Harvey rat sarcoma virus oncogene Mus musculus 41-46 7585519-6 1995 These data demonstrate that, even though H-ras-transformed cells accumulate up to 20 fold the concentration of RA as NIH-3T3 cells, they fail to show transglutaminase induction, suggesting that H-ras interferes with signal transduction by RA. Tretinoin 111-113 Harvey rat sarcoma virus oncogene Mus musculus 194-199 7585519-6 1995 These data demonstrate that, even though H-ras-transformed cells accumulate up to 20 fold the concentration of RA as NIH-3T3 cells, they fail to show transglutaminase induction, suggesting that H-ras interferes with signal transduction by RA. Tretinoin 239-241 Harvey rat sarcoma virus oncogene Mus musculus 41-46 7482563-8 1995 The frequency and pattern of H-ras gene activation were similar in control and methylene chloride-induced liver neoplasms. Methylene Chloride 79-97 Harvey rat sarcoma virus oncogene Mus musculus 29-34 7479797-0 1995 Relating aromatic hydrocarbon-induced DNA adducts and c-H-ras mutations in mouse skin papillomas: the role of apurinic sites. Hydrocarbons 18-29 Harvey rat sarcoma virus oncogene Mus musculus 54-61 7554075-6 1995 TPA- and mirex-promoted papillomas that were refractory to RA and FA demonstrated the same incidence of Ha-ras mutation as TPA- or mirex-promoted papillomas without RA and FA treatment, further indicating that the inhibitory activity of RA and FA is promoter-dependent and not solely dependent on mutant Ha-ras. Tetradecanoylphorbol Acetate 0-3 Harvey rat sarcoma virus oncogene Mus musculus 104-110 7554075-6 1995 TPA- and mirex-promoted papillomas that were refractory to RA and FA demonstrated the same incidence of Ha-ras mutation as TPA- or mirex-promoted papillomas without RA and FA treatment, further indicating that the inhibitory activity of RA and FA is promoter-dependent and not solely dependent on mutant Ha-ras. Tetradecanoylphorbol Acetate 0-3 Harvey rat sarcoma virus oncogene Mus musculus 304-310 7554075-6 1995 TPA- and mirex-promoted papillomas that were refractory to RA and FA demonstrated the same incidence of Ha-ras mutation as TPA- or mirex-promoted papillomas without RA and FA treatment, further indicating that the inhibitory activity of RA and FA is promoter-dependent and not solely dependent on mutant Ha-ras. Mirex 9-14 Harvey rat sarcoma virus oncogene Mus musculus 104-110 7554075-6 1995 TPA- and mirex-promoted papillomas that were refractory to RA and FA demonstrated the same incidence of Ha-ras mutation as TPA- or mirex-promoted papillomas without RA and FA treatment, further indicating that the inhibitory activity of RA and FA is promoter-dependent and not solely dependent on mutant Ha-ras. Mirex 9-14 Harvey rat sarcoma virus oncogene Mus musculus 304-310 7544154-6 1995 These data indicate that epidermal expression of v-fos induces sensitivity to TPA promotion, but since additional genetic events, such as endogenous c-rasHa activation, appear to be required in tumorigenesis, v-fos may predominantly play a role in the mechanism of promotion to achieve papilloma autonomy and TPA independence. Tetradecanoylphorbol Acetate 78-81 Harvey rat sarcoma virus oncogene Mus musculus 149-156 7627957-4 1995 Molecular analysis of these tumors revealed that the majority of papillomas (17/20) and carcinomas (9/10) showed DMBA-specific mutations (A to T transversion at the 61st codon) in the Ha-ras gene. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 113-117 Harvey rat sarcoma virus oncogene Mus musculus 184-190 7659512-1 1995 An oligodeoxyribonucleotide containing 8-hydroxyadenine (OH8Ade) was chemically synthesized and single- and double-stranded c-Ha-ras gene fragments with OH8Ade at the second position of codon 61 were prepared. oh8ade 153-159 Harvey rat sarcoma virus oncogene Mus musculus 124-132 7659512-5 1995 The c-Ha-ras gene was constructed using the double-stranded ras gene fragment containing OH8Ade and was transfected into NIH 3T3 cells. oh8ade 89-95 Harvey rat sarcoma virus oncogene Mus musculus 4-12 7634393-0 1995 H-ras oncogene mutation spectra in B6C3F1 and C57BL/6 mouse liver tumors provide evidence for TCDD promotion of spontaneous and vinyl carbamate-initiated liver cells. Polychlorinated Dibenzodioxins 94-98 Harvey rat sarcoma virus oncogene Mus musculus 0-5 7634393-0 1995 H-ras oncogene mutation spectra in B6C3F1 and C57BL/6 mouse liver tumors provide evidence for TCDD promotion of spontaneous and vinyl carbamate-initiated liver cells. Carbamates 134-143 Harvey rat sarcoma virus oncogene Mus musculus 0-5 7634393-6 1995 Fifty-one percent of tumors analyzed from B6C3F1 mice treated with TCDD alone had H-ras codon 61 mutations with a pattern similar to that detected in spontaneous tumors. Polychlorinated Dibenzodioxins 67-71 Harvey rat sarcoma virus oncogene Mus musculus 82-87 7606738-5 1995 These profound changes in papilloma frequency and growth occurred in the absence of the characteristic DMBA-induced A182-->T mutation in c-Ha-ras and immunohistochemical nuclear staining for p53 protein. 9,10-Dimethyl-1,2-benzanthracene 103-107 Harvey rat sarcoma virus oncogene Mus musculus 140-148 7585889-1 1995 The effect of an induction of transforming Ha-ras on Ca2+ influx into NIH3T3 cells was studied employing Fura-2 quenching by Mn2+. Fura-2 105-111 Harvey rat sarcoma virus oncogene Mus musculus 43-49 7585889-1 1995 The effect of an induction of transforming Ha-ras on Ca2+ influx into NIH3T3 cells was studied employing Fura-2 quenching by Mn2+. Manganese(2+) 125-129 Harvey rat sarcoma virus oncogene Mus musculus 43-49 7585889-8 1995 The mitogenic response to an expression of transforming Ha-ras was inhibited by the Ca(2+)-channel blockers not, however, by charybdotoxin. Charybdotoxin 125-138 Harvey rat sarcoma virus oncogene Mus musculus 56-62 7598718-0 1995 Suppression of anionic amino acid transport impairs the maintenance of intracellular glutamate in Ha-ras-expressing cells. Glutamic Acid 85-94 Harvey rat sarcoma virus oncogene Mus musculus 98-104 7598718-1 1995 When the expression of a Ha-ras oncogene is triggered in NIH3T3 cells, a progressive inhibition of sodium dependent transport of anionic amino acids through system X-AG is observed. Sodium 99-105 Harvey rat sarcoma virus oncogene Mus musculus 25-31 7673348-1 1995 Rho protein (rho p21), a p21ras-related small guanine nucleotide binding protein, regulates cytoskeletal organization in a number of different types of cells. Guanine Nucleotides 46-64 Harvey rat sarcoma virus oncogene Mus musculus 25-31 7744871-8 1995 The results indicate that the specific binding of ATF3/Jun and a previously uncharacterized factor account for signal-specific transcription in response to EGF or an activated Ha-ras gene in a cell type in which the cooperative action of an activated Ha-ras gene and 12-O-tetradecanoylphorbol-13-acetate cause tumor growth. Tetradecanoylphorbol Acetate 267-303 Harvey rat sarcoma virus oncogene Mus musculus 176-182 7744871-8 1995 The results indicate that the specific binding of ATF3/Jun and a previously uncharacterized factor account for signal-specific transcription in response to EGF or an activated Ha-ras gene in a cell type in which the cooperative action of an activated Ha-ras gene and 12-O-tetradecanoylphorbol-13-acetate cause tumor growth. Tetradecanoylphorbol Acetate 267-303 Harvey rat sarcoma virus oncogene Mus musculus 251-257 7544154-6 1995 These data indicate that epidermal expression of v-fos induces sensitivity to TPA promotion, but since additional genetic events, such as endogenous c-rasHa activation, appear to be required in tumorigenesis, v-fos may predominantly play a role in the mechanism of promotion to achieve papilloma autonomy and TPA independence. Tetradecanoylphorbol Acetate 309-312 Harvey rat sarcoma virus oncogene Mus musculus 149-156 7728970-0 1995 8-Hydroxyguanine (7,8-dihydro-8-oxoguanine) in hot spots of the c-Ha-ras gene: effects of sequence contexts on mutation spectra. 8-hydroxyguanine 0-16 Harvey rat sarcoma virus oncogene Mus musculus 64-72 7721808-0 1995 Prostaglandin F2 alpha stimulates formation of p21ras-GTP complex and mitogen-activated protein kinase in NIH-3T3 cells via Gq-protein-coupled pathway. Dinoprost 0-22 Harvey rat sarcoma virus oncogene Mus musculus 47-53 7721808-0 1995 Prostaglandin F2 alpha stimulates formation of p21ras-GTP complex and mitogen-activated protein kinase in NIH-3T3 cells via Gq-protein-coupled pathway. Guanosine Triphosphate 54-57 Harvey rat sarcoma virus oncogene Mus musculus 47-53 7728970-0 1995 8-Hydroxyguanine (7,8-dihydro-8-oxoguanine) in hot spots of the c-Ha-ras gene: effects of sequence contexts on mutation spectra. 7,8-dihydro-8-oxoguanine 18-42 Harvey rat sarcoma virus oncogene Mus musculus 64-72 7728944-3 1995 Direct DNA sequencing of the Ha-ras retrieved from the foci revealed that most mutations (23/24, 96%) took place at dipyrimidine sequences, and the C-->T transition at the 3"-cytosine in 5"-TC or 5"-CC sequences was predominant (17/24, 71%) in codons 12, 13 and 60. dipyrimidine 116-128 Harvey rat sarcoma virus oncogene Mus musculus 29-35 7728944-3 1995 Direct DNA sequencing of the Ha-ras retrieved from the foci revealed that most mutations (23/24, 96%) took place at dipyrimidine sequences, and the C-->T transition at the 3"-cytosine in 5"-TC or 5"-CC sequences was predominant (17/24, 71%) in codons 12, 13 and 60. 3"-cytosine 175-186 Harvey rat sarcoma virus oncogene Mus musculus 29-35 7728970-1 1995 We previously reported that 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) at the second position of codon 12 of the c-Ha-ras gene induces many types of mutations in NIH3T3 cells. 8-hydroxyguanine 28-44 Harvey rat sarcoma virus oncogene Mus musculus 114-122 7728970-1 1995 We previously reported that 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) at the second position of codon 12 of the c-Ha-ras gene induces many types of mutations in NIH3T3 cells. 7,8-dihydro-8-oxoguanine 46-70 Harvey rat sarcoma virus oncogene Mus musculus 114-122 7662118-0 1995 Ha-ras p21-GTP levels remain constant during primary keratinocyte differentiation. Guanosine Triphosphate 11-14 Harvey rat sarcoma virus oncogene Mus musculus 0-6 7728944-3 1995 Direct DNA sequencing of the Ha-ras retrieved from the foci revealed that most mutations (23/24, 96%) took place at dipyrimidine sequences, and the C-->T transition at the 3"-cytosine in 5"-TC or 5"-CC sequences was predominant (17/24, 71%) in codons 12, 13 and 60. 5"-tc 190-195 Harvey rat sarcoma virus oncogene Mus musculus 29-35 7697804-7 1995 In contrast, tumors with H-ras mutations from DCA-treated mice revealed a H-61 change from CAA to AAA in 21% at 3.5 g/l and 16% at 1.0 g/l. dichloroacetylene 46-49 Harvey rat sarcoma virus oncogene Mus musculus 25-30 7697804-10 1995 The data indicates that induction of liver carcinoma by DCA and TCA involves activation of the H-ras proto-oncogene at a frequency similar to that observed in spontaneous tumors. dichloroacetylene 56-59 Harvey rat sarcoma virus oncogene Mus musculus 95-100 7697804-10 1995 The data indicates that induction of liver carcinoma by DCA and TCA involves activation of the H-ras proto-oncogene at a frequency similar to that observed in spontaneous tumors. Trichloroacetic Acid 64-67 Harvey rat sarcoma virus oncogene Mus musculus 95-100 7727042-2 1995 We used 7,12-dimethylbenz[a]anthracene-initiated and 12-O-tetradecanoylphorbol-13-acetate-promoted SENCAR mouse skin tumors, which were shown to contain Ha-ras oncogene activated by point mutation at codon 61, as an in vivo model for these studies. 7,12-dimethylbenz[a 8-27 Harvey rat sarcoma virus oncogene Mus musculus 153-159 7727042-2 1995 We used 7,12-dimethylbenz[a]anthracene-initiated and 12-O-tetradecanoylphorbol-13-acetate-promoted SENCAR mouse skin tumors, which were shown to contain Ha-ras oncogene activated by point mutation at codon 61, as an in vivo model for these studies. Tetradecanoylphorbol Acetate 53-89 Harvey rat sarcoma virus oncogene Mus musculus 153-159 7727042-5 1995 Compared with saline-treated animals, in which tumor growth continued, lovastatin-treated animals had significantly inhibited tumor growth, which led to tumor regression with concomitant inhibition of Ha-ras p21 membrane localization. Lovastatin 71-81 Harvey rat sarcoma virus oncogene Mus musculus 201-207 7662118-4 1995 In the present studies, we measured the level and activity state of Ha-ras p21 protein in cultured keratinocytes undergoing calcium-induced differentiation. Calcium 124-131 Harvey rat sarcoma virus oncogene Mus musculus 68-74 7662118-7 1995 The percentage of Ha-ras p21 protein in its active, GTP-bound form also remained unchanged during primary adult keratinocyte differentiation and in immortalized, benign, and malignant keratinocytes subjected to differentiating conditions. Guanosine Triphosphate 52-55 Harvey rat sarcoma virus oncogene Mus musculus 18-24 7774440-2 1995 It was a complementary oligonucleotide chain to the sequence of initiation position of c-Ha-ras gene transcription. Oligonucleotides 23-38 Harvey rat sarcoma virus oncogene Mus musculus 87-95 7829473-1 1995 Insulin receptor signaling acutely stimulates GTP loading of p21ras, apparently by mobilizing complexes of Grb2 and the guanine nucleotide exchangers Son-of-sevenless (Sos) 1 and 2 to associate with tyrosine-phosphorylated proteins in the plasma membrane. Guanosine Triphosphate 46-49 Harvey rat sarcoma virus oncogene Mus musculus 61-67 7829473-1 1995 Insulin receptor signaling acutely stimulates GTP loading of p21ras, apparently by mobilizing complexes of Grb2 and the guanine nucleotide exchangers Son-of-sevenless (Sos) 1 and 2 to associate with tyrosine-phosphorylated proteins in the plasma membrane. Guanine Nucleotides 120-138 Harvey rat sarcoma virus oncogene Mus musculus 61-67 7829473-1 1995 Insulin receptor signaling acutely stimulates GTP loading of p21ras, apparently by mobilizing complexes of Grb2 and the guanine nucleotide exchangers Son-of-sevenless (Sos) 1 and 2 to associate with tyrosine-phosphorylated proteins in the plasma membrane. Tyrosine 199-207 Harvey rat sarcoma virus oncogene Mus musculus 61-67 7829473-2 1995 Here we show that in 32P-labeled 3T3-L1 adipocytes the elevated cellular concentrations of [32P]GTP-bound p21ras in response to insulin return to near basal levels after 20-30 min of hormone stimulation, while insulin receptors remain activated. Phosphorus-32 21-24 Harvey rat sarcoma virus oncogene Mus musculus 106-112 7829473-2 1995 Here we show that in 32P-labeled 3T3-L1 adipocytes the elevated cellular concentrations of [32P]GTP-bound p21ras in response to insulin return to near basal levels after 20-30 min of hormone stimulation, while insulin receptors remain activated. Phosphorus-32 92-95 Harvey rat sarcoma virus oncogene Mus musculus 106-112 7829473-2 1995 Here we show that in 32P-labeled 3T3-L1 adipocytes the elevated cellular concentrations of [32P]GTP-bound p21ras in response to insulin return to near basal levels after 20-30 min of hormone stimulation, while insulin receptors remain activated. Guanosine Triphosphate 96-99 Harvey rat sarcoma virus oncogene Mus musculus 106-112 7818528-6 1995 Highly purified CDC25Mm 1005-1260, expressed in E. coli using the pMAL system, enhanced the GDP release from both H-ras p21 and S. cerevisiae Ras2p and its activity was nearly as high as that of CDC25Mm 974-1260. Guanosine Diphosphate 92-95 Harvey rat sarcoma virus oncogene Mus musculus 114-119 7960226-0 1994 Hepatic tumors induced by carbon tetrachloride in transgenic mice carrying a human c-H-ras proto-oncogene without mutations. Carbon Tetrachloride 26-46 Harvey rat sarcoma virus oncogene Mus musculus 85-90 7890805-3 1994 The signal is likely independent of protein kinase C, but depends on tyrosine kinase and other kinase activities and is mediated by c-Ha-Ras since the presence of dominant-negative mutants of Ras and Raf abrogates the induction of Egr-1 expression by Zn2+. Zinc 251-255 Harvey rat sarcoma virus oncogene Mus musculus 132-140 7525585-3 1994 This tyrosine phosphorylation correlated with an increased association with the GTPase-activating protein of p21ras (GAP). Tyrosine 5-13 Harvey rat sarcoma virus oncogene Mus musculus 109-115 7525612-0 1994 Ornithine decarboxylase gene expression is aberrantly regulated via the cAMP signal transduction pathway in malignant H-ras transformed cell lines. Cyclic AMP 72-76 Harvey rat sarcoma virus oncogene Mus musculus 118-123 7969452-2 1994 Tyrosine-phosphorylated IRS-1 binds the 85K subunit of phosphatidylinositol 3-kinase which may be involved in the translocation of glucose transporters and the abundant src homology protein (ASH)/Grb2 which may be involved in activation of p21ras and MAP kinase cascade. Tyrosine 0-8 Harvey rat sarcoma virus oncogene Mus musculus 240-246 7955123-0 1994 Activation of H-ras is prevalent in 1,3-butadiene-induced and spontaneously occurring murine Harderian gland tumors. 1,3-butadiene 36-49 Harvey rat sarcoma virus oncogene Mus musculus 14-19 7525612-1 1994 We have tested the hypothesis that H-ras transformed cells contain alterations in signal pathways important in controlling the expression of ornithine decarboxylase (ODC), the highly regulated rate-limiting activity in the biosynthesis of polyamines. Polyamines 239-249 Harvey rat sarcoma virus oncogene Mus musculus 35-40 7525612-9 1994 In addition, treatment of H-ras transformed cells with the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA) led to an elevation in ODC mRNA levels not observed in parental 10T1/2 fibroblasts. Tetradecanoylphorbol Acetate 75-111 Harvey rat sarcoma virus oncogene Mus musculus 26-31 7525612-9 1994 In addition, treatment of H-ras transformed cells with the tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA) led to an elevation in ODC mRNA levels not observed in parental 10T1/2 fibroblasts. Tetradecanoylphorbol Acetate 113-116 Harvey rat sarcoma virus oncogene Mus musculus 26-31 7523375-5 1994 Steroid treatment (dexamethasone, 1 microM) specifically induced c-Ha-ras(Asn-17) protein and mRNA and blocked IL-3-induced accumulation of p21ras-GTP in 32Dcl3/p21rasN17 cell lines, but not in control cells. Guanosine Triphosphate 147-150 Harvey rat sarcoma virus oncogene Mus musculus 140-146 7937975-0 1994 Antisense oligonucleotides adsorbed to polyalkylcyanoacrylate nanoparticles specifically inhibit mutated Ha-ras-mediated cell proliferation and tumorigenicity in nude mice. Oligonucleotides 10-26 Harvey rat sarcoma virus oncogene Mus musculus 105-111 7937975-0 1994 Antisense oligonucleotides adsorbed to polyalkylcyanoacrylate nanoparticles specifically inhibit mutated Ha-ras-mediated cell proliferation and tumorigenicity in nude mice. polyalkylcyanoacrylate 39-61 Harvey rat sarcoma virus oncogene Mus musculus 105-111 7937975-5 1994 Nanoparticle-adsorbed antisense oligonucleotides directed to a point mutation (G-->U) in codon 12 of the Ha-ras mRNA selectively inhibited the proliferation of cells expressing the point-mutated Ha-ras gene at a concentration 100 times lower than free oligonucleotides. Oligonucleotides 32-48 Harvey rat sarcoma virus oncogene Mus musculus 108-114 7937975-5 1994 Nanoparticle-adsorbed antisense oligonucleotides directed to a point mutation (G-->U) in codon 12 of the Ha-ras mRNA selectively inhibited the proliferation of cells expressing the point-mutated Ha-ras gene at a concentration 100 times lower than free oligonucleotides. Oligonucleotides 32-48 Harvey rat sarcoma virus oncogene Mus musculus 198-204 7937975-5 1994 Nanoparticle-adsorbed antisense oligonucleotides directed to a point mutation (G-->U) in codon 12 of the Ha-ras mRNA selectively inhibited the proliferation of cells expressing the point-mutated Ha-ras gene at a concentration 100 times lower than free oligonucleotides. Oligonucleotides 255-271 Harvey rat sarcoma virus oncogene Mus musculus 108-114 7923158-0 1994 Harvey ras (H-ras) point mutations are induced by 4-nitroquinoline-1-oxide in murine oral squamous epithelia, while squamous cell carcinomas and loss of heterozygosity occur without additional exposure. 4-Nitroquinoline-1-oxide 50-74 Harvey rat sarcoma virus oncogene Mus musculus 0-10 7923158-0 1994 Harvey ras (H-ras) point mutations are induced by 4-nitroquinoline-1-oxide in murine oral squamous epithelia, while squamous cell carcinomas and loss of heterozygosity occur without additional exposure. 4-Nitroquinoline-1-oxide 50-74 Harvey rat sarcoma virus oncogene Mus musculus 12-17 7523375-5 1994 Steroid treatment (dexamethasone, 1 microM) specifically induced c-Ha-ras(Asn-17) protein and mRNA and blocked IL-3-induced accumulation of p21ras-GTP in 32Dcl3/p21rasN17 cell lines, but not in control cells. Steroids 0-7 Harvey rat sarcoma virus oncogene Mus musculus 67-73 7523375-5 1994 Steroid treatment (dexamethasone, 1 microM) specifically induced c-Ha-ras(Asn-17) protein and mRNA and blocked IL-3-induced accumulation of p21ras-GTP in 32Dcl3/p21rasN17 cell lines, but not in control cells. Steroids 0-7 Harvey rat sarcoma virus oncogene Mus musculus 140-146 7945447-3 1994 H-ras-transformed cells had altered morphology and increased colony formation in soft agar in contrast to untransfected 3T3 cells. Agar 86-90 Harvey rat sarcoma virus oncogene Mus musculus 0-5 7523375-5 1994 Steroid treatment (dexamethasone, 1 microM) specifically induced c-Ha-ras(Asn-17) protein and mRNA and blocked IL-3-induced accumulation of p21ras-GTP in 32Dcl3/p21rasN17 cell lines, but not in control cells. Dexamethasone 19-32 Harvey rat sarcoma virus oncogene Mus musculus 67-73 7523375-5 1994 Steroid treatment (dexamethasone, 1 microM) specifically induced c-Ha-ras(Asn-17) protein and mRNA and blocked IL-3-induced accumulation of p21ras-GTP in 32Dcl3/p21rasN17 cell lines, but not in control cells. Dexamethasone 19-32 Harvey rat sarcoma virus oncogene Mus musculus 140-146 7945447-8 1994 Insertion of a mutant ribozyme largely devoid of cleaving capacity into H-ras-transformed cells resulted in smaller reductions in H-ras gene expression and colony formation in soft agar when compared with the ras ribozyme. Agar 181-185 Harvey rat sarcoma virus oncogene Mus musculus 72-77 8077052-0 1994 Differential Na+,K(+)-ATPase activity and cisplatin sensitivity between transformants induced by H-ras and those induced by K-ras. Cisplatin 42-51 Harvey rat sarcoma virus oncogene Mus musculus 97-102 7916997-2 1994 More than 95% of tumors induced by initiation with 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) exhibited mutations in Ha-ras and trisomy of chromosome 7. 9,10-Dimethyl-1,2-benzanthracene 51-81 Harvey rat sarcoma virus oncogene Mus musculus 175-181 7916997-2 1994 More than 95% of tumors induced by initiation with 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) exhibited mutations in Ha-ras and trisomy of chromosome 7. 9,10-Dimethyl-1,2-benzanthracene 83-87 Harvey rat sarcoma virus oncogene Mus musculus 175-181 7916997-2 1994 More than 95% of tumors induced by initiation with 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) exhibited mutations in Ha-ras and trisomy of chromosome 7. Tetradecanoylphorbol Acetate 108-145 Harvey rat sarcoma virus oncogene Mus musculus 175-181 7916997-2 1994 More than 95% of tumors induced by initiation with 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoyl-phorbol-13-acetate (TPA) exhibited mutations in Ha-ras and trisomy of chromosome 7. Tetradecanoylphorbol Acetate 147-150 Harvey rat sarcoma virus oncogene Mus musculus 175-181 7916997-3 1994 Carcinomas induced with multiple DMBA treatments had a lower frequency of alterations on chromosome 7 (50%), but only in tumors with Ha-ras mutations, and had a much wider spectrum of alterations, including trisomy, mitotic recombination, deletion, and gene duplication. 9,10-Dimethyl-1,2-benzanthracene 33-37 Harvey rat sarcoma virus oncogene Mus musculus 133-139 7916997-4 1994 Carcinomas induced with multiple N-methyl-N"-nitro-N-nitrosoguanidine treatments only rarely exhibited alterations on chromosome 7 (8%), even if they contained mutant Ha-ras. Methylnitronitrosoguanidine 33-69 Harvey rat sarcoma virus oncogene Mus musculus 167-173 7955041-8 1994 Mutations in codon 61 of the Ha-ras gene were common, however, in mouse skin tumors induced by this diol-epoxide, being detected in 63% of the tumors analyzed; 90% of these mutations were CAA-->CTA. diol-epoxide 100-112 Harvey rat sarcoma virus oncogene Mus musculus 29-35 7955041-10 1994 A CAA-->CTA mutation in codon 61 of Ha-ras was also detected in one of four acetone control tumors. Acetone 79-86 Harvey rat sarcoma virus oncogene Mus musculus 39-45 7955041-11 1994 In comparison with previous studies of other polynuclear aromatic hydrocarbons and their metabolites, the results suggest that the reactivity with DNA of anti-B[c]PhDE is one factor involved in the induction of A mutations in Ha-ras genes in mouse skin, but further studies are required to evaluate the significance of these mutations in mouse skin tumorigenesis. Polycyclic Aromatic Hydrocarbons 45-78 Harvey rat sarcoma virus oncogene Mus musculus 226-232 7955063-3 1994 The mutation spectra of H-ras codon 61 mutations showed a significant decrease in AAA and increase in CTA mutations for dichloroacetic acid- and trichloroethylene-induced tumors when compared to combined controls. Dichloroacetic Acid 120-139 Harvey rat sarcoma virus oncogene Mus musculus 24-29 7955063-3 1994 The mutation spectra of H-ras codon 61 mutations showed a significant decrease in AAA and increase in CTA mutations for dichloroacetic acid- and trichloroethylene-induced tumors when compared to combined controls. Trichloroethylene 145-162 Harvey rat sarcoma virus oncogene Mus musculus 24-29 7955063-4 1994 The H-ras codon 61 mutation frequency for tetrachloroethylene-induced tumors was significantly lower (24%) than that of combined controls and also that of the two other chemicals. Tetrachloroethylene 42-61 Harvey rat sarcoma virus oncogene Mus musculus 4-9 7955063-5 1994 Mutations at codons 13 and 117 plus a second exon insert contributed 4% to the total H-ras frequencies for trichloroethylene and tetrachloroethylene. Trichloroethylene 107-124 Harvey rat sarcoma virus oncogene Mus musculus 85-90 7955063-5 1994 Mutations at codons 13 and 117 plus a second exon insert contributed 4% to the total H-ras frequencies for trichloroethylene and tetrachloroethylene. Tetrachloroethylene 129-148 Harvey rat sarcoma virus oncogene Mus musculus 85-90 7955063-8 1994 These findings suggest that exposure to dichloroacetic acid, trichloroethylene and tetrachloroethylene provides a selective growth advantage to spontaneously occurring mutations in codon 61 of H-ras and, at the same time, is responsible for a small number of unique molecular lesions suggestive of either a random genotoxic mode of action or a non-specific result of secondary DNA damage. Dichloroacetic Acid 40-59 Harvey rat sarcoma virus oncogene Mus musculus 193-198 7955063-8 1994 These findings suggest that exposure to dichloroacetic acid, trichloroethylene and tetrachloroethylene provides a selective growth advantage to spontaneously occurring mutations in codon 61 of H-ras and, at the same time, is responsible for a small number of unique molecular lesions suggestive of either a random genotoxic mode of action or a non-specific result of secondary DNA damage. Trichloroethylene 61-78 Harvey rat sarcoma virus oncogene Mus musculus 193-198 7955063-8 1994 These findings suggest that exposure to dichloroacetic acid, trichloroethylene and tetrachloroethylene provides a selective growth advantage to spontaneously occurring mutations in codon 61 of H-ras and, at the same time, is responsible for a small number of unique molecular lesions suggestive of either a random genotoxic mode of action or a non-specific result of secondary DNA damage. Tetrachloroethylene 83-102 Harvey rat sarcoma virus oncogene Mus musculus 193-198 7712129-3 1994 Some of the more potent olefinic compounds inhibit Ras processing in intact v-ras transformed NIH 3T3 cells with IC50 values in the 0.1 to 1 microM range, and inhibit selectively the anchorage-independent growth of H-ras transformed Rat1 cells at 10 microM. olefinic 24-32 Harvey rat sarcoma virus oncogene Mus musculus 215-220 8077052-2 1994 Although the NIH/3T3 cells transformed with H-ras oncogenes (EJ-NIH/3T3 and Ha8-21) showed an increased resistance to cisplatin compared to the parental NIH/3T3, the cell lines transformed with K-ras oncogenes (DT and 1,8DNP2-2-5) did not. Cisplatin 118-127 Harvey rat sarcoma virus oncogene Mus musculus 44-49 8077052-2 1994 Although the NIH/3T3 cells transformed with H-ras oncogenes (EJ-NIH/3T3 and Ha8-21) showed an increased resistance to cisplatin compared to the parental NIH/3T3, the cell lines transformed with K-ras oncogenes (DT and 1,8DNP2-2-5) did not. Thymidine 211-213 Harvey rat sarcoma virus oncogene Mus musculus 44-49 8077052-2 1994 Although the NIH/3T3 cells transformed with H-ras oncogenes (EJ-NIH/3T3 and Ha8-21) showed an increased resistance to cisplatin compared to the parental NIH/3T3, the cell lines transformed with K-ras oncogenes (DT and 1,8DNP2-2-5) did not. 1,8dnp2-2-5 218-229 Harvey rat sarcoma virus oncogene Mus musculus 44-49 8077052-3 1994 Compared with NIH/3T3, the 2 H-ras transformants reduced both the accumulation of cisplatin and the Na+,K(+)-ATPase activity in the membrane fraction. Cisplatin 82-91 Harvey rat sarcoma virus oncogene Mus musculus 29-34 21559551-4 1994 CAPE displayed increased growth suppressive activity toward CREF cells transformed by a number of oncogenes, including Ha-ras, v-src, v-raf, human papillomavirus type 18 (HPV-18) and human papillomavirus type 51 (HPV-51). caffeic acid phenethyl ester 0-4 Harvey rat sarcoma virus oncogene Mus musculus 119-125 8063767-0 1994 Role of p21ras in insulin-stimulated glucose transport in 3T3-L1 adipocytes. Glucose 37-44 Harvey rat sarcoma virus oncogene Mus musculus 8-14 8063767-2 1994 This study examines the role of p21ras in the signaling pathways by which insulin increases hexose transport in differentiated 3T3-L1 adipose cells, a model system for study of the metabolic effects of the hormone on a physiological target tissue. Hexoses 92-98 Harvey rat sarcoma virus oncogene Mus musculus 32-38 21559551-6 1994 Ha-ras/Krev-1 cells displaying a suppression of the transformed phenotype exhibited increased resistance to CAPE-induced growth suppression versus Ha-ras cells, whereas Ha-ras/Krev-1 cells escaping transformation-suppression following in vivo growth in nude mice displayed enhanced sensitivity to growth-suppression induced by CAPE. caffeic acid phenethyl ester 108-112 Harvey rat sarcoma virus oncogene Mus musculus 0-6 21559551-6 1994 Ha-ras/Krev-1 cells displaying a suppression of the transformed phenotype exhibited increased resistance to CAPE-induced growth suppression versus Ha-ras cells, whereas Ha-ras/Krev-1 cells escaping transformation-suppression following in vivo growth in nude mice displayed enhanced sensitivity to growth-suppression induced by CAPE. caffeic acid phenethyl ester 327-331 Harvey rat sarcoma virus oncogene Mus musculus 0-6 8197195-4 1994 The NIH 3T3 cells used in this study contained mutant p53 genes and carried a selectively inducible activated (EJ) Ha-ras transgene under the control of bacterial lactose regulatory elements. Lactose 163-170 Harvey rat sarcoma virus oncogene Mus musculus 115-121 8197195-5 1994 When stably transfected cells were induced to express activated Ha-ras by isopropyl beta-D-thiogalactoside administration, there was a marked increase in the number of gross chromosomal aberrations including acentric fragments, multicentric chromosomes, and double minutes, which occurred within the time frame of a single cell cycle from the time of induction. Isopropyl Thiogalactoside 74-106 Harvey rat sarcoma virus oncogene Mus musculus 64-70 8162600-0 1994 Increased methotrexate resistance and dhfr gene amplification as a consequence of induced Ha-ras expression in NIH 3T3 cells. Methotrexate 10-22 Harvey rat sarcoma virus oncogene Mus musculus 90-96 8162600-3 1994 To better understand the relationship between expression of an oncogene and genetic instability, we have studied a cell line expressing an activated human Ha-ras under the control of bacterial lactose operon regulatory elements for changes in methotrexate resistance and dihydrofolate reductase (dhfr) gene amplification following mutant Ha-ras induction. Lactose 193-200 Harvey rat sarcoma virus oncogene Mus musculus 155-161 8200073-0 1994 Low frequency of H-ras mutations in hepatocellular adenomas and carcinomas and in hepatoblastomas from B6C3F1 mice exposed to oxazepam in the diet. Oxazepam 126-134 Harvey rat sarcoma virus oncogene Mus musculus 17-22 8162600-3 1994 To better understand the relationship between expression of an oncogene and genetic instability, we have studied a cell line expressing an activated human Ha-ras under the control of bacterial lactose operon regulatory elements for changes in methotrexate resistance and dihydrofolate reductase (dhfr) gene amplification following mutant Ha-ras induction. Methotrexate 243-255 Harvey rat sarcoma virus oncogene Mus musculus 155-161 8162600-5 1994 The expression of this Ha-ras is specifically induced by the addition of isopropyl-1-thio-beta-D-galactopyranoside (IPTG), a lactose analogue, to the culture medium. Isopropyl Thiogalactoside 73-114 Harvey rat sarcoma virus oncogene Mus musculus 23-29 8162600-5 1994 The expression of this Ha-ras is specifically induced by the addition of isopropyl-1-thio-beta-D-galactopyranoside (IPTG), a lactose analogue, to the culture medium. Isopropyl Thiogalactoside 116-120 Harvey rat sarcoma virus oncogene Mus musculus 23-29 8162600-5 1994 The expression of this Ha-ras is specifically induced by the addition of isopropyl-1-thio-beta-D-galactopyranoside (IPTG), a lactose analogue, to the culture medium. Lactose 125-132 Harvey rat sarcoma virus oncogene Mus musculus 23-29 8162600-9 1994 The increased capacity to amplify DNA in response to mutant Ha-ras induction was not locus specific since cells also displayed an increased frequency of resistance to N-(phosphonacetyl)-L-aspartic acid in the presence of ITPG. sparfosic acid 167-201 Harvey rat sarcoma virus oncogene Mus musculus 60-66 8043512-4 1994 Transgenic skin was initiated with a single dose of 7,12-dimethylbenz[a]anthracene (DMBA), shown previously to induce, in concert with a tumor promoter, murine papillomas that consistently contain specific H-ras mutations. 9,10-Dimethyl-1,2-benzanthracene 52-82 Harvey rat sarcoma virus oncogene Mus musculus 206-211 8043512-4 1994 Transgenic skin was initiated with a single dose of 7,12-dimethylbenz[a]anthracene (DMBA), shown previously to induce, in concert with a tumor promoter, murine papillomas that consistently contain specific H-ras mutations. 9,10-Dimethyl-1,2-benzanthracene 84-88 Harvey rat sarcoma virus oncogene Mus musculus 206-211 8016216-0 1994 Pyrimidine dimer induction and removal in the epidermis of hairless mice: inefficient repair in the genome overall and rapid repair in the H-ras sequence. pyrimidine 0-10 Harvey rat sarcoma virus oncogene Mus musculus 139-144 8122111-4 1994 Activated G alpha s and 8-Br-cAMP suppressed H-Ras-induced transformation of NIH 3T3 cells. 8-Bromo Cyclic Adenosine Monophosphate 24-33 Harvey rat sarcoma virus oncogene Mus musculus 45-50 7511643-2 1994 This event is accompanied by the tyrosine phosphorylation of the p21ras-associated GTPase-activating protein p120 ras.GAP, raising the possibility that AgR-stimulated p21ras activity is regulated by protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPases) in B cells. Tyrosine 33-41 Harvey rat sarcoma virus oncogene Mus musculus 65-71 7511643-2 1994 This event is accompanied by the tyrosine phosphorylation of the p21ras-associated GTPase-activating protein p120 ras.GAP, raising the possibility that AgR-stimulated p21ras activity is regulated by protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPases) in B cells. Tyrosine 33-41 Harvey rat sarcoma virus oncogene Mus musculus 167-173 8312255-2 1994 The sequence represents a portion of the mouse c-Ha-ras protooncogene and was selectively modified to contain a single N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF) adduct at the deoxyguanosine corresponding to the first base of codon 61. N-(deoxyguanosin-8-yl)-2-aminofluorene 119-157 Harvey rat sarcoma virus oncogene Mus musculus 47-55 8176761-7 1994 The increase in neurofibromin levels paralleled a decrease in the levels of activated p21-ras as assayed by in vivo 32P-orthophosphate incorporation into p21-ras. 32p-orthophosphate 116-134 Harvey rat sarcoma virus oncogene Mus musculus 86-93 8176761-7 1994 The increase in neurofibromin levels paralleled a decrease in the levels of activated p21-ras as assayed by in vivo 32P-orthophosphate incorporation into p21-ras. 32p-orthophosphate 116-134 Harvey rat sarcoma virus oncogene Mus musculus 154-161 8312255-2 1994 The sequence represents a portion of the mouse c-Ha-ras protooncogene and was selectively modified to contain a single N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF) adduct at the deoxyguanosine corresponding to the first base of codon 61. Deoxyguanosine 183-197 Harvey rat sarcoma virus oncogene Mus musculus 47-55 8307971-0 1994 Long-term expression of c-H-ras stimulates Na-H and Na(+)-dependent Cl-HCO3 exchange in NIH-3T3 fibroblasts. cl-hco3 68-75 Harvey rat sarcoma virus oncogene Mus musculus 24-31 8313527-0 1994 H-ras 61st codon activation in archival proliferative hepatic lesions isolated from female B6C3F1 mice exposed to the leukotriene D4-antagonist, LY171883. Leukotriene D4 118-132 Harvey rat sarcoma virus oncogene Mus musculus 0-5 8313527-0 1994 H-ras 61st codon activation in archival proliferative hepatic lesions isolated from female B6C3F1 mice exposed to the leukotriene D4-antagonist, LY171883. LY 171883 145-153 Harvey rat sarcoma virus oncogene Mus musculus 0-5 8313527-9 1994 It may be concluded that based on the similarity in mutation spectrum and the increase in mutation frequency, LY171883 may selectively promote spontaneous hepatic lesions containing H-ras 61st codon mutations. LY 171883 110-118 Harvey rat sarcoma virus oncogene Mus musculus 182-187 8060898-9 1994 METHOD: Antisense oligonucleotides directed to the point mutation (G to T) in codon 12 of the Ha-ras mRNA were adsorbed to nanoparticles in the presence of hydrophobic cations. Oligonucleotides 18-34 Harvey rat sarcoma virus oncogene Mus musculus 94-100 8142011-1 1994 Distinct differences have been described in the development of C3H/He mouse liver tumors induced by the genotoxic carcinogen diethylnitrosamine (DEN) and by the nongenotoxic agent phenobarbitone (PB) in terms of pathology and the frequency of mutation at codon 61 of the Ha-ras oncogene. Diethylnitrosamine 145-148 Harvey rat sarcoma virus oncogene Mus musculus 271-277 8142011-1 1994 Distinct differences have been described in the development of C3H/He mouse liver tumors induced by the genotoxic carcinogen diethylnitrosamine (DEN) and by the nongenotoxic agent phenobarbitone (PB) in terms of pathology and the frequency of mutation at codon 61 of the Ha-ras oncogene. Phenobarbital 180-194 Harvey rat sarcoma virus oncogene Mus musculus 271-277 21566909-0 1994 Increased p21ras-specific Guanine-nucleotide exchange causes tumor-formation in nude-mice. Guanine Nucleotides 26-44 Harvey rat sarcoma virus oncogene Mus musculus 10-16 7942278-2 1994 Expression of Ha-ras by dexamethasone leads to a transcriptional activation of the fos-CAT construct which was found to be sensitive to the PKC inhibitor ilmofosine (BM41440) and abrogated by PKC depletion following long-term exposure to TPA. Dexamethasone 24-37 Harvey rat sarcoma virus oncogene Mus musculus 14-20 7942278-2 1994 Expression of Ha-ras by dexamethasone leads to a transcriptional activation of the fos-CAT construct which was found to be sensitive to the PKC inhibitor ilmofosine (BM41440) and abrogated by PKC depletion following long-term exposure to TPA. ilmofosine 154-164 Harvey rat sarcoma virus oncogene Mus musculus 14-20 7942278-2 1994 Expression of Ha-ras by dexamethasone leads to a transcriptional activation of the fos-CAT construct which was found to be sensitive to the PKC inhibitor ilmofosine (BM41440) and abrogated by PKC depletion following long-term exposure to TPA. ilmofosine 166-173 Harvey rat sarcoma virus oncogene Mus musculus 14-20 7942278-2 1994 Expression of Ha-ras by dexamethasone leads to a transcriptional activation of the fos-CAT construct which was found to be sensitive to the PKC inhibitor ilmofosine (BM41440) and abrogated by PKC depletion following long-term exposure to TPA. Tetradecanoylphorbol Acetate 238-241 Harvey rat sarcoma virus oncogene Mus musculus 14-20 7942278-5 1994 Transcriptional activation of the SRE-FAP-TK-CAT as well as the SRE-TK-CAT constructs by Ha-ras is sensitive to the PKC-inhibitor ilmofosine (BM41440) and blocked by long-term exposure to TPA. ilmofosine 130-140 Harvey rat sarcoma virus oncogene Mus musculus 89-95 7942278-5 1994 Transcriptional activation of the SRE-FAP-TK-CAT as well as the SRE-TK-CAT constructs by Ha-ras is sensitive to the PKC-inhibitor ilmofosine (BM41440) and blocked by long-term exposure to TPA. ilmofosine 142-149 Harvey rat sarcoma virus oncogene Mus musculus 89-95 7942278-5 1994 Transcriptional activation of the SRE-FAP-TK-CAT as well as the SRE-TK-CAT constructs by Ha-ras is sensitive to the PKC-inhibitor ilmofosine (BM41440) and blocked by long-term exposure to TPA. Tetradecanoylphorbol Acetate 188-191 Harvey rat sarcoma virus oncogene Mus musculus 89-95 8297482-5 1994 In male rats, a single administration of 30 mg/kg furan produced necrosis and a subsequent wave of cell proliferation 48 h after treatment and induced transient peaks in the expression of myc, fos, and Ha-ras 6-24 h after treatment. furan 50-55 Harvey rat sarcoma virus oncogene Mus musculus 202-208 7505797-11 1994 Thus, p210bcr-abl appears to function in a hematopoietic p21ras activation pathway to allow growth factor-independent coupling between Grb2, which exists in a complex with the guanine nucleotide exchange factor (Sos), and p21ras. sulfur monoxide 212-215 Harvey rat sarcoma virus oncogene Mus musculus 57-63 8297482-0 1994 Expression of myc, fos, and Ha-ras in the livers of furan-treated F344 rats and B6C3F1 mice. furan 52-57 Harvey rat sarcoma virus oncogene Mus musculus 28-34 8297482-4 1994 Quantitative northern blot analysis of mRNA was used to examine the expression of the oncogenes myc, fos, and Ha-ras in the livers of animals treated with furan. furan 155-160 Harvey rat sarcoma virus oncogene Mus musculus 110-116 7505277-0 1994 Alterations in the cyclic AMP signal transduction pathway regulating ribonucleotide reductase gene expression in malignant H-ras transformed cell lines. Cyclic AMP 19-29 Harvey rat sarcoma virus oncogene Mus musculus 123-128 7505277-7 1994 Also, a direct relationship between H-ras expression and ribonucleotide reductase gene expression was observed; analysis of forskolin mediated elevations in R1 and R2 message levels closely correlated with the levels of H-ras expression in the various cell lines. Colforsin 124-133 Harvey rat sarcoma virus oncogene Mus musculus 220-225 8297482-9 1994 The absence of an increase in Ha-ras expression in the male mouse liver suggests that the unique pattern of Ha-ras mutations previously reported in furan-induced mouse liver tumors is not due to increased mutational susceptibility related to overexpression of this gene. furan 148-153 Harvey rat sarcoma virus oncogene Mus musculus 108-114 7693388-0 1993 Cyclic AMP decreases chemotaxis, invasiveness and lung colonization of H-ras transformed mouse fibroblasts. Cyclic AMP 0-10 Harvey rat sarcoma virus oncogene Mus musculus 71-76 8242857-0 1993 Characterization of benzo[a]pyrene-initiated mouse skin papillomas for Ha-ras mutations and protein kinase C levels. Benzo(a)pyrene 20-34 Harvey rat sarcoma virus oncogene Mus musculus 71-77 8242857-1 1993 The frequency and spectrum of Ha-ras mutations in benzo[a]pyrene (B[a]P)-initiated/12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted CD-1 mouse skin papillomas were characterized by amplifying high molecular weight papilloma DNA using the polymerase chain reaction (PCR) followed by direct DNA sequencing. Benzo(a)pyrene 50-64 Harvey rat sarcoma virus oncogene Mus musculus 30-36 8242857-1 1993 The frequency and spectrum of Ha-ras mutations in benzo[a]pyrene (B[a]P)-initiated/12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted CD-1 mouse skin papillomas were characterized by amplifying high molecular weight papilloma DNA using the polymerase chain reaction (PCR) followed by direct DNA sequencing. Benzo(a)pyrene 66-71 Harvey rat sarcoma virus oncogene Mus musculus 30-36 8242857-1 1993 The frequency and spectrum of Ha-ras mutations in benzo[a]pyrene (B[a]P)-initiated/12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted CD-1 mouse skin papillomas were characterized by amplifying high molecular weight papilloma DNA using the polymerase chain reaction (PCR) followed by direct DNA sequencing. Tetradecanoylphorbol Acetate 83-119 Harvey rat sarcoma virus oncogene Mus musculus 30-36 8269640-0 1993 Diacylglycerol is an effector of the clonal expansion of cells containing activated Ha-ras genes. Diglycerides 0-14 Harvey rat sarcoma virus oncogene Mus musculus 84-90 8269640-3 1993 We now report that 11/12 of DMBA-initiated/DIC10-promoted papillomas examined contain an A-->T mutation in the 61st codon of the Ha-ras gene, suggesting that DAGs affect the clonal expansion of activated Ha-ras-containing cells. 9,10-Dimethyl-1,2-benzanthracene 28-32 Harvey rat sarcoma virus oncogene Mus musculus 132-138 8269640-8 1993 These data implicate DAG as an effector of the clonal expansion of mutated Ha-ras-containing cells, and support a mechanism whereby an increase in endogenous DAG could contribute to the clonal expansion of cells containing a Ha-ras oncogene. Diglycerides 21-24 Harvey rat sarcoma virus oncogene Mus musculus 75-81 8269640-8 1993 These data implicate DAG as an effector of the clonal expansion of mutated Ha-ras-containing cells, and support a mechanism whereby an increase in endogenous DAG could contribute to the clonal expansion of cells containing a Ha-ras oncogene. Diglycerides 158-161 Harvey rat sarcoma virus oncogene Mus musculus 225-231 8242857-1 1993 The frequency and spectrum of Ha-ras mutations in benzo[a]pyrene (B[a]P)-initiated/12-O-tetradecanoylphorbol-13-acetate (TPA)-promoted CD-1 mouse skin papillomas were characterized by amplifying high molecular weight papilloma DNA using the polymerase chain reaction (PCR) followed by direct DNA sequencing. Tetradecanoylphorbol Acetate 121-124 Harvey rat sarcoma virus oncogene Mus musculus 30-36 7691239-5 1993 Inhibition of p210 bcr-abl-dependent tyrosine phosphorylation resulted in a reduction of active p21RAS-GTP complexes in the transformed cells, in diminished expression of the nuclear early response genes c-jun and c-fos, and in lower cellular proliferation rate. Guanosine Triphosphate 103-106 Harvey rat sarcoma virus oncogene Mus musculus 96-102 8242857-4 1993 A characteristic of 7,12-dimethylbenz[a]anthracene (DMBA)-initiated papillomas, which contain an A-->T mutation in the 61st codon of Ha-ras, is that they exhibit a constitutive decrease in both protein kinase C (PKC) activity and PKC alpha and beta 2 isozyme levels when compared to epidermis. 7,12-dimethylbenz[a 20-39 Harvey rat sarcoma virus oncogene Mus musculus 136-142 8242857-4 1993 A characteristic of 7,12-dimethylbenz[a]anthracene (DMBA)-initiated papillomas, which contain an A-->T mutation in the 61st codon of Ha-ras, is that they exhibit a constitutive decrease in both protein kinase C (PKC) activity and PKC alpha and beta 2 isozyme levels when compared to epidermis. anthracene 40-50 Harvey rat sarcoma virus oncogene Mus musculus 136-142 8242857-4 1993 A characteristic of 7,12-dimethylbenz[a]anthracene (DMBA)-initiated papillomas, which contain an A-->T mutation in the 61st codon of Ha-ras, is that they exhibit a constitutive decrease in both protein kinase C (PKC) activity and PKC alpha and beta 2 isozyme levels when compared to epidermis. 9,10-Dimethyl-1,2-benzanthracene 52-56 Harvey rat sarcoma virus oncogene Mus musculus 136-142 8228821-4 1993 First, stimulation of TA3 7.9 Ag-specific murine B lymphoma cells for 2 min with either Ag or F(ab")2 anti-IgM induces p21ras activation as measured by an increase in the GTP/GDP ratio of its bound nucleotides. Guanosine Triphosphate 171-174 Harvey rat sarcoma virus oncogene Mus musculus 119-125 8228821-4 1993 First, stimulation of TA3 7.9 Ag-specific murine B lymphoma cells for 2 min with either Ag or F(ab")2 anti-IgM induces p21ras activation as measured by an increase in the GTP/GDP ratio of its bound nucleotides. Guanosine Diphosphate 175-178 Harvey rat sarcoma virus oncogene Mus musculus 119-125 8240360-3 1993 Both cytochrome c and ferricyanide are reduced at a faster rate by C3H10T1/2 cells which are expressing the Ha-ras oncogene. hexacyanoferrate III 22-34 Harvey rat sarcoma virus oncogene Mus musculus 108-114 7691239-5 1993 Inhibition of p210 bcr-abl-dependent tyrosine phosphorylation resulted in a reduction of active p21RAS-GTP complexes in the transformed cells, in diminished expression of the nuclear early response genes c-jun and c-fos, and in lower cellular proliferation rate. Tyrosine 37-45 Harvey rat sarcoma virus oncogene Mus musculus 96-102 7694287-4 1993 Two of the interacting tyrosine-phosphorylated proteins were identified as the p85 subunit of phosphatidylinositol 3-kinase and the GTPase-activating protein of p21ras. Tyrosine 23-31 Harvey rat sarcoma virus oncogene Mus musculus 161-167 8378348-0 1993 GTP-dependent association of Raf-1 with Ha-Ras: identification of Raf as a target downstream of Ras in mammalian cells. Guanosine Triphosphate 0-3 Harvey rat sarcoma virus oncogene Mus musculus 40-46 8378348-4 1993 In an effort to identify the Ras-associated downstream proteins, we added recombinant Ha-Ras in a GTP-bound form to cell-free lysates and used several antibodies against Ras to immunoprecipitate Ras complexes. Guanosine Triphosphate 98-101 Harvey rat sarcoma virus oncogene Mus musculus 86-92 8378348-7 1993 The GTP-dependent association of Ha-Ras with Raf-1 was observed with lysates of various types of cultured cells, including NIH 3T3, pheochromocytoma (PC) 12, Ba/F3, and Jurkat T cells, and also with crude extracts from rat brain. Guanosine Triphosphate 4-7 Harvey rat sarcoma virus oncogene Mus musculus 33-39 8354267-5 1993 The small GTP-binding protein Ha-ras was reduced by 50%, whereas rab1 and other small GTP-binding proteins tentatively identified as rab-isoforms (ras-homologous gene products from brain) were increased by 100% and 70%, respectively. Guanosine Triphosphate 10-13 Harvey rat sarcoma virus oncogene Mus musculus 30-36 8353844-1 1993 In a previous study, the spectrum of H-ras mutations detected in B6C3F1 mouse liver tumors induced by 5, 50 or 150 mumol/kg body wt of N-nitrosodiethylamine (NDEA) was similar to that in spontaneous B6C3F1 mouse liver tumors, suggesting that activation of the H-ras gene in NDEA-induced mouse liver tumors may not be the direct result of the chemical interaction with the H-ras gene. Diethylnitrosamine 135-156 Harvey rat sarcoma virus oncogene Mus musculus 37-42 8353844-3 1993 Twenty-one of 66 NDEA-induced B6C3F1 mouse liver tumors contained activated H-ras gene with 2 of 21 having a CG to AT transversion at the first base of codon 61, 17 of 21 having AT to GC transition and 2 of 21 having an AT to TA transversion at the second base of codon 61 in the H-ras gene. Diethylnitrosamine 17-21 Harvey rat sarcoma virus oncogene Mus musculus 76-81 8353844-3 1993 Twenty-one of 66 NDEA-induced B6C3F1 mouse liver tumors contained activated H-ras gene with 2 of 21 having a CG to AT transversion at the first base of codon 61, 17 of 21 having AT to GC transition and 2 of 21 having an AT to TA transversion at the second base of codon 61 in the H-ras gene. Diethylnitrosamine 17-21 Harvey rat sarcoma virus oncogene Mus musculus 280-285 8359224-9 1993 This concentration of TPEN totally blocked DNA synthesis both in control and c-Ha-ras(val-12)-expressing fibroblasts. N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine 22-26 Harvey rat sarcoma virus oncogene Mus musculus 77-85 8359224-9 1993 This concentration of TPEN totally blocked DNA synthesis both in control and c-Ha-ras(val-12)-expressing fibroblasts. val-12 86-92 Harvey rat sarcoma virus oncogene Mus musculus 77-85 8359224-12 1993 The results suggest that c-Ha-ras(val-12)-induced proliferation is independent of changes in [Ca2+]i. val-12 34-40 Harvey rat sarcoma virus oncogene Mus musculus 25-33 8509220-5 1993 NIH-3T3 transformants expressing H-ras were less sensitive than those expressing trk or the wild type to the indoloquinone EO9, methotrexate and arabino-furanosylcytosine. Indolequinones 109-122 Harvey rat sarcoma virus oncogene Mus musculus 33-38 12595920-56 1993 The mutation spectrum of the H-ras genes in the relatively few neoplasms from exposed mice that did have an activated H-ras did not differ from the spectrum of mutations observed in neoplasms from controls, but the proportion of neoplasms with an activated H-ras gene decreased with increasing oxazepam dose. Oxazepam 294-302 Harvey rat sarcoma virus oncogene Mus musculus 29-34 8508502-9 1993 Finally, > 90% of DMBA-initiated/mirex-promoted papillomas from male mice and female mice demonstrated a mutated Ha-ras gene with an A-->T transversion in the middle base of the 61st codon. 9,10-Dimethyl-1,2-benzanthracene 21-25 Harvey rat sarcoma virus oncogene Mus musculus 116-122 8508502-9 1993 Finally, > 90% of DMBA-initiated/mirex-promoted papillomas from male mice and female mice demonstrated a mutated Ha-ras gene with an A-->T transversion in the middle base of the 61st codon. Mirex 36-41 Harvey rat sarcoma virus oncogene Mus musculus 116-122 8509220-5 1993 NIH-3T3 transformants expressing H-ras were less sensitive than those expressing trk or the wild type to the indoloquinone EO9, methotrexate and arabino-furanosylcytosine. apaziquone 123-126 Harvey rat sarcoma virus oncogene Mus musculus 33-38 8509220-5 1993 NIH-3T3 transformants expressing H-ras were less sensitive than those expressing trk or the wild type to the indoloquinone EO9, methotrexate and arabino-furanosylcytosine. Methotrexate 128-140 Harvey rat sarcoma virus oncogene Mus musculus 33-38 8509220-5 1993 NIH-3T3 transformants expressing H-ras were less sensitive than those expressing trk or the wild type to the indoloquinone EO9, methotrexate and arabino-furanosylcytosine. Cytarabine 145-170 Harvey rat sarcoma virus oncogene Mus musculus 33-38 8504471-6 1993 This mutation profile was similar to that identified for the H-ras gene in the spontaneous liver tumors and suggests that methylene chloride acts in liver by promoting cells with spontaneous lesions. Methylene Chloride 122-140 Harvey rat sarcoma virus oncogene Mus musculus 61-66 8455619-1 1993 Although p21ras is localized to the plasma membrane, proteins it interacts with, such as the GTPase-activating proteins (GAPs) ras GAP and neurofibromin (NF1), are not, suggesting that one function of p21ras GTP may be to target such proteins to the plasma membrane. Guanosine Triphosphate 93-96 Harvey rat sarcoma virus oncogene Mus musculus 9-15 8334304-0 1993 Activation of the mitogen-activated protein kinase pathway in Triton X-100 disrupted NIH-3T3 cells by p21 ras and in vitro by plasma membranes from NIH 3T3 cells. Octoxynol 62-74 Harvey rat sarcoma virus oncogene Mus musculus 102-109 8459217-5 1993 Activation of p21ras is deficient in quiescent and stimulated NOD T cells, and this is correlated with a significant reduction in the tyrosine phosphorylation of p42mapk, a serine/threonine kinase active downstream of p21ras. Tyrosine 134-142 Harvey rat sarcoma virus oncogene Mus musculus 14-20 8459217-6 1993 Treatment of NOD T cells with a phorbol ester not only enhances their p21ras activity and p42mapk tyrosine phosphorylation but also restores their proliferative responsiveness. Phorbol Esters 32-45 Harvey rat sarcoma virus oncogene Mus musculus 70-76 8280375-0 1993 Further analysis of c-Ha-ras mutations in papillomas initiated by several polycyclic aromatic hydrocarbons and papillomas from uninitiated, promoter-treated skin in SENCAR mice. Polycyclic Aromatic Hydrocarbons 74-106 Harvey rat sarcoma virus oncogene Mus musculus 20-28 8425263-4 1993 Screening of another 23 ciprofibrate-induced liver tumors by oligonucleotide hybridization analysis and direct DNA sequencing resulted in the identification of three tumor DNA samples with point mutations in codon 117 of the H-ras gene. ciprofibrate 24-36 Harvey rat sarcoma virus oncogene Mus musculus 225-230 8425263-4 1993 Screening of another 23 ciprofibrate-induced liver tumors by oligonucleotide hybridization analysis and direct DNA sequencing resulted in the identification of three tumor DNA samples with point mutations in codon 117 of the H-ras gene. Oligonucleotides 61-76 Harvey rat sarcoma virus oncogene Mus musculus 225-230 7681292-5 1993 Previous studies have suggested that the induction of K13 in mouse skin is related to the mutation of the Ha-ras gene by the initiating agent DMBA, a mutation consistently found in murine DMBA/TPA-induced tumors and rarely found in human skin tumors. 9,10-Dimethyl-1,2-benzanthracene 142-146 Harvey rat sarcoma virus oncogene Mus musculus 106-112 7681292-5 1993 Previous studies have suggested that the induction of K13 in mouse skin is related to the mutation of the Ha-ras gene by the initiating agent DMBA, a mutation consistently found in murine DMBA/TPA-induced tumors and rarely found in human skin tumors. Tetradecanoylphorbol Acetate 193-196 Harvey rat sarcoma virus oncogene Mus musculus 106-112 8380225-0 1993 The Saccharomyces cerevisiae SDC25 C-domain gene product overcomes the dominant inhibitory activity of Ha-Ras Asn-17. Asparagine 110-113 Harvey rat sarcoma virus oncogene Mus musculus 103-109 8380225-4 1993 Transcription from the polyomavirus thymidine kinase gene (Py tk) promoter is strongly inhibited by the expression of Ha-Ras Asn-17 in NIH 3T3 cells. Asparagine 125-128 Harvey rat sarcoma virus oncogene Mus musculus 118-124 8380225-6 1993 On the other hand, transactivation of the Ras-responsive element of the Py tk promoter induced by SDC25 C domain is lost upon coexpression of increasing amounts of Ha-Ras Asn-17. Asparagine 171-174 Harvey rat sarcoma virus oncogene Mus musculus 164-170 8380225-7 1993 In addition, coexpression of SDC25 C domain overcomes the inhibition of proliferation of NIH 3T3 cells caused by Ha-Ras Asn-17. Asparagine 120-123 Harvey rat sarcoma virus oncogene Mus musculus 113-119 8380225-8 1993 These results are consistent with the idea that the Ha-Ras Asn-17 mutant functions by titrating an upstream activator of cellular Ras proteins. Asparagine 59-62 Harvey rat sarcoma virus oncogene Mus musculus 52-58 7681161-0 1993 Increased levels of p21ras-GTP and enhanced DNA synthesis accompany elevated tyrosyl phosphorylation of GAP-associated proteins, p190 and p62, in c-src overexpressors. Guanosine Triphosphate 27-30 Harvey rat sarcoma virus oncogene Mus musculus 20-26 7681161-0 1993 Increased levels of p21ras-GTP and enhanced DNA synthesis accompany elevated tyrosyl phosphorylation of GAP-associated proteins, p190 and p62, in c-src overexpressors. cyclo(tyrosyl-tyrosyl) 77-84 Harvey rat sarcoma virus oncogene Mus musculus 20-26 7681161-20 1993 However, analysis of GTP-bound p21ras revealed higher basal and EGF-stimulated levels in c-src overexpressors than in control cells. Guanosine Triphosphate 21-24 Harvey rat sarcoma virus oncogene Mus musculus 31-37 7992552-3 1993 In the same cell model, lovastatin, an inhibitor of beta hydroxy-beta methyl-glutaryl-CoA-reductase and, hence, of farnesylation of p21ras, partially protects aspartate transport from the inhibition observed upon steroid treatment. Lovastatin 24-34 Harvey rat sarcoma virus oncogene Mus musculus 132-138 7992552-3 1993 In the same cell model, lovastatin, an inhibitor of beta hydroxy-beta methyl-glutaryl-CoA-reductase and, hence, of farnesylation of p21ras, partially protects aspartate transport from the inhibition observed upon steroid treatment. Aspartic Acid 159-168 Harvey rat sarcoma virus oncogene Mus musculus 132-138 7992552-3 1993 In the same cell model, lovastatin, an inhibitor of beta hydroxy-beta methyl-glutaryl-CoA-reductase and, hence, of farnesylation of p21ras, partially protects aspartate transport from the inhibition observed upon steroid treatment. Steroids 213-220 Harvey rat sarcoma virus oncogene Mus musculus 132-138 7992552-6 1993 It is proposed i) that cell production of oncogenic p21ras hinders sodium-dependent transport of anionic amino acids and ii) that the transport alteration impairs the maintenance of cell levels of glutamate in ras-expressing cells. Sodium 67-73 Harvey rat sarcoma virus oncogene Mus musculus 52-58 7992552-6 1993 It is proposed i) that cell production of oncogenic p21ras hinders sodium-dependent transport of anionic amino acids and ii) that the transport alteration impairs the maintenance of cell levels of glutamate in ras-expressing cells. Glutamic Acid 197-206 Harvey rat sarcoma virus oncogene Mus musculus 52-58 7681292-5 1993 Previous studies have suggested that the induction of K13 in mouse skin is related to the mutation of the Ha-ras gene by the initiating agent DMBA, a mutation consistently found in murine DMBA/TPA-induced tumors and rarely found in human skin tumors. 9,10-Dimethyl-1,2-benzanthracene 188-192 Harvey rat sarcoma virus oncogene Mus musculus 106-112 8280375-1 1993 In this study we analyzed the mutations in c-Ha-ras from skin papillomas initiated with benzo[a]pyrene (B[a]P), 7-methylbenz[a]anthracene (7-MBA), and 10-fluoro-7-methylbenz[a]anthracene (10-F-7-MBA) and from papillomas induced by treatment with tumor promoter alone. Phosphorus 108-109 Harvey rat sarcoma virus oncogene Mus musculus 43-51 8280375-1 1993 In this study we analyzed the mutations in c-Ha-ras from skin papillomas initiated with benzo[a]pyrene (B[a]P), 7-methylbenz[a]anthracene (7-MBA), and 10-fluoro-7-methylbenz[a]anthracene (10-F-7-MBA) and from papillomas induced by treatment with tumor promoter alone. 7-methylbenz 112-124 Harvey rat sarcoma virus oncogene Mus musculus 43-51 8280375-1 1993 In this study we analyzed the mutations in c-Ha-ras from skin papillomas initiated with benzo[a]pyrene (B[a]P), 7-methylbenz[a]anthracene (7-MBA), and 10-fluoro-7-methylbenz[a]anthracene (10-F-7-MBA) and from papillomas induced by treatment with tumor promoter alone. anthracene 127-137 Harvey rat sarcoma virus oncogene Mus musculus 43-51 8280375-1 1993 In this study we analyzed the mutations in c-Ha-ras from skin papillomas initiated with benzo[a]pyrene (B[a]P), 7-methylbenz[a]anthracene (7-MBA), and 10-fluoro-7-methylbenz[a]anthracene (10-F-7-MBA) and from papillomas induced by treatment with tumor promoter alone. anthracene 176-186 Harvey rat sarcoma virus oncogene Mus musculus 43-51 8280375-1 1993 In this study we analyzed the mutations in c-Ha-ras from skin papillomas initiated with benzo[a]pyrene (B[a]P), 7-methylbenz[a]anthracene (7-MBA), and 10-fluoro-7-methylbenz[a]anthracene (10-F-7-MBA) and from papillomas induced by treatment with tumor promoter alone. 10-f-7-mba 188-198 Harvey rat sarcoma virus oncogene Mus musculus 43-51 8280375-1 1993 In this study we analyzed the mutations in c-Ha-ras from skin papillomas initiated with benzo[a]pyrene (B[a]P), 7-methylbenz[a]anthracene (7-MBA), and 10-fluoro-7-methylbenz[a]anthracene (10-F-7-MBA) and from papillomas induced by treatment with tumor promoter alone. Benzo(a)pyrene 88-102 Harvey rat sarcoma virus oncogene Mus musculus 43-51 1448068-1 1992 We have used a dominant inhibitory ras mutant (Ha-ras Asn-17) to investigate the relationship of Ras proteins to hydrolysis of phosphatidylcholine (PC) in the transduction of mitogenic signals. Asparagine 54-57 Harvey rat sarcoma virus oncogene Mus musculus 47-53 1361883-7 1992 The frequency of H-ras codon 61 mutations in tumors induced by 0.15 mumol/g body weight VC in the C57BL/6J mouse (5/37) was similar to that in spontaneous tumors (2/9); surprisingly, tumors induced by a lower dose of VC (0.03 mumol/g body weight) had a higher frequency of H-ras mutations (12/28). vinyl carbamate 88-90 Harvey rat sarcoma virus oncogene Mus musculus 17-22 1425589-1 1992 It has been shown previously in T cells that stimulation of protein kinase C or the T cell antigen receptor leads to a rapid and persistent activation of p21ras as measured by a dramatic increase in the amount of bound GTP. Guanosine Triphosphate 219-222 Harvey rat sarcoma virus oncogene Mus musculus 154-160 1425589-5 1992 In the murine T cell line EL4, constitutively active p21ras greatly potentiates the phorbol ester and T cell receptor agonist induced production of IL-2 as measured both by biological assay for the cytokine and by the use of a reporter construct. Phorbol Esters 84-97 Harvey rat sarcoma virus oncogene Mus musculus 53-59 1425589-6 1992 Active p21ras also partially replaces the requirement for protein kinase C activation in synergizing with a calcium ionophore to induce production of IL-2. Calcium 108-115 Harvey rat sarcoma virus oncogene Mus musculus 7-13 1448068-2 1992 Expression of Ha-Ras Asn-17 inhibited NIH 3T3 cell proliferation induced by polypeptide growth factors or phorbol esters. asn-17 21-27 Harvey rat sarcoma virus oncogene Mus musculus 14-20 1448068-2 1992 Expression of Ha-Ras Asn-17 inhibited NIH 3T3 cell proliferation induced by polypeptide growth factors or phorbol esters. Phorbol Esters 106-120 Harvey rat sarcoma virus oncogene Mus musculus 14-20 1423878-0 1992 Inhibition of mouse skin tumorigenesis by dexamethasone occurs through a Ha-ras-independent mechanism. Dexamethasone 42-55 Harvey rat sarcoma virus oncogene Mus musculus 73-79 1423878-1 1992 The Ha-ras oncogene has been shown to be point-mutated and overexpressed in papillomas induced by the two-stage skin tumorigenesis regimen of 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoylphorbol-13-acetate (TPA). 9,10-Dimethyl-1,2-benzanthracene 174-178 Harvey rat sarcoma virus oncogene Mus musculus 4-10 1423878-1 1992 The Ha-ras oncogene has been shown to be point-mutated and overexpressed in papillomas induced by the two-stage skin tumorigenesis regimen of 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoylphorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 199-235 Harvey rat sarcoma virus oncogene Mus musculus 4-10 1423878-1 1992 The Ha-ras oncogene has been shown to be point-mutated and overexpressed in papillomas induced by the two-stage skin tumorigenesis regimen of 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoylphorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 237-240 Harvey rat sarcoma virus oncogene Mus musculus 4-10 1423878-1 1992 The Ha-ras oncogene has been shown to be point-mutated and overexpressed in papillomas induced by the two-stage skin tumorigenesis regimen of 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoylphorbol-13-acetate (TPA). 7,12-dimethylbenz 142-159 Harvey rat sarcoma virus oncogene Mus musculus 4-10 1423878-1 1992 The Ha-ras oncogene has been shown to be point-mutated and overexpressed in papillomas induced by the two-stage skin tumorigenesis regimen of 7,12-dimethylbenz[a]anthracene (DMBA) and promotion with 12-O-tetradecanoylphorbol-13-acetate (TPA). anthracene 162-172 Harvey rat sarcoma virus oncogene Mus musculus 4-10 1408146-4 1992 H1 histamine receptors were identified in both v-K-ras- and v-H-ras-transformed Balb/3T3 cells, but not in untransformed cells, using the specific H1 antagonist [3H]-pyrilamine. Tritium 162-164 Harvey rat sarcoma virus oncogene Mus musculus 62-67 1423854-0 1992 Search for Ha-ras codon 61 mutations in liver tumours caused by hexachlorobenzene and Aroclor 1254 in C57BL/10ScSn mice with iron overload. Hexachlorobenzene 64-81 Harvey rat sarcoma virus oncogene Mus musculus 11-17 1423854-0 1992 Search for Ha-ras codon 61 mutations in liver tumours caused by hexachlorobenzene and Aroclor 1254 in C57BL/10ScSn mice with iron overload. Aroclors 86-93 Harvey rat sarcoma virus oncogene Mus musculus 11-17 1423854-0 1992 Search for Ha-ras codon 61 mutations in liver tumours caused by hexachlorobenzene and Aroclor 1254 in C57BL/10ScSn mice with iron overload. Iron 125-129 Harvey rat sarcoma virus oncogene Mus musculus 11-17 1423854-2 1992 A range of lesions from the livers were analysed for the presence of mutations in the Ha-ras proto-oncogene at codon 61 using the polymerase chain reaction to amplify DNA from formalin-fixed sections, followed by oligonucleotide hybridization. Formaldehyde 176-184 Harvey rat sarcoma virus oncogene Mus musculus 86-92 1408146-4 1992 H1 histamine receptors were identified in both v-K-ras- and v-H-ras-transformed Balb/3T3 cells, but not in untransformed cells, using the specific H1 antagonist [3H]-pyrilamine. Pyrilamine 166-176 Harvey rat sarcoma virus oncogene Mus musculus 62-67 1511406-0 1992 Analysis of the Harvey ras gene in cisplatin-initiated mouse skin tumors by polymerase chain reaction and direct DNA sequencing. Cisplatin 35-44 Harvey rat sarcoma virus oncogene Mus musculus 16-26 1508192-0 1992 Platelet-derived growth factor stimulation of GTPase-activating protein tyrosine phosphorylation in control and c-H-ras-expressing NIH 3T3 cells correlates with p21ras activation. Tyrosine 72-80 Harvey rat sarcoma virus oncogene Mus musculus 112-119 1508192-0 1992 Platelet-derived growth factor stimulation of GTPase-activating protein tyrosine phosphorylation in control and c-H-ras-expressing NIH 3T3 cells correlates with p21ras activation. Tyrosine 72-80 Harvey rat sarcoma virus oncogene Mus musculus 161-167 1508192-5 1992 Furthermore, PDGF exposure led to a rapid and sustained increase in the levels of p21ras bound to GTP, with kinetics similar to those observed for GAP tyrosine phosphorylation. Guanosine Triphosphate 98-101 Harvey rat sarcoma virus oncogene Mus musculus 82-88 1508192-6 1992 The PDGF-induced increases in GTP-bound p21ras in NIH 3T3 cells were comparable to the steady-state level observed in serum-starved c-H-ras-overexpressing transformants, conditions in which these cells maintained high rates of DNA synthesis. Guanosine Triphosphate 30-33 Harvey rat sarcoma virus oncogene Mus musculus 40-46 1508192-8 1992 Addition of PDGF to c-H-ras-overexpressing cells also resulted in a rapid and sustained increase in GTP-bound p21ras. Guanosine Triphosphate 100-103 Harvey rat sarcoma virus oncogene Mus musculus 20-27 1508192-8 1992 Addition of PDGF to c-H-ras-overexpressing cells also resulted in a rapid and sustained increase in GTP-bound p21ras. Guanosine Triphosphate 100-103 Harvey rat sarcoma virus oncogene Mus musculus 110-116 1435769-8 1992 The vanadate-stimulated expression of actin and c-Ha-ras mRNA were unaffected by oxidants, reductants, metal chelators, or anti-oxidant enzymes. Vanadates 4-12 Harvey rat sarcoma virus oncogene Mus musculus 48-56 1435769-10 1992 The alternate hypothesis that the actions of vanadate on actin and c-Ha-ras were mediated by a protein kinase cascade was inconsistent with the following observations. Vanadates 45-53 Harvey rat sarcoma virus oncogene Mus musculus 67-75 1511406-3 1992 Since the Harvey-ras (H-ras) gene is known to be activated by point mutations in chemically initiated mouse skin tumors, we used polymerase chain reaction (PCR) and direct DNA sequencing to analyze the DNA sequence of the H-ras gene in twelve different cisplatin-initiated skin tumors. Cisplatin 253-262 Harvey rat sarcoma virus oncogene Mus musculus 10-20 1511406-3 1992 Since the Harvey-ras (H-ras) gene is known to be activated by point mutations in chemically initiated mouse skin tumors, we used polymerase chain reaction (PCR) and direct DNA sequencing to analyze the DNA sequence of the H-ras gene in twelve different cisplatin-initiated skin tumors. Cisplatin 253-262 Harvey rat sarcoma virus oncogene Mus musculus 22-27 1511406-3 1992 Since the Harvey-ras (H-ras) gene is known to be activated by point mutations in chemically initiated mouse skin tumors, we used polymerase chain reaction (PCR) and direct DNA sequencing to analyze the DNA sequence of the H-ras gene in twelve different cisplatin-initiated skin tumors. Cisplatin 253-262 Harvey rat sarcoma virus oncogene Mus musculus 222-227 1638698-0 1992 Temporal changes in the mutant frequency and mutation spectra of the 61st codon of the H-ras oncogene following exposure of B6C3F1 mice to N-nitrosodiethylamine (DEN). Diethylnitrosamine 139-160 Harvey rat sarcoma virus oncogene Mus musculus 87-92 1352887-5 1992 Activated Ha-ras genes with 61st codon A----T mutations were found in the epidermis of mice 1 week after topical initiation with 7,12-dimethylbenz[a]anthracene or urethane by using this assay. 7,12-dimethylbenz[a 129-148 Harvey rat sarcoma virus oncogene Mus musculus 10-16 1352887-5 1992 Activated Ha-ras genes with 61st codon A----T mutations were found in the epidermis of mice 1 week after topical initiation with 7,12-dimethylbenz[a]anthracene or urethane by using this assay. anthracene 149-159 Harvey rat sarcoma virus oncogene Mus musculus 10-16 1352887-5 1992 Activated Ha-ras genes with 61st codon A----T mutations were found in the epidermis of mice 1 week after topical initiation with 7,12-dimethylbenz[a]anthracene or urethane by using this assay. Urethane 163-171 Harvey rat sarcoma virus oncogene Mus musculus 10-16 1404241-2 1992 Differences in c-ras expression between control and TCDD treated groups were determined by immunoassay of p21ras protein, or indirectly measured by the specific binding of 3H-GTP to hepatic plasma membrane preparations. Polychlorinated Dibenzodioxins 52-56 Harvey rat sarcoma virus oncogene Mus musculus 106-112 1404241-6 1992 In contrast, TCDD tolerant DBA/2J mice had only a marginal increase in hepatic p21ras protein which did not become statistically significant even at 24 hr host-dosing. Polychlorinated Dibenzodioxins 13-17 Harvey rat sarcoma virus oncogene Mus musculus 79-85 1404241-7 1992 TCDD evoked increases in hepatic p21ras protein of C57BL/6J mice were accompanied by the increase in the specific binding of GTP to hepatic plasma membranes. Polychlorinated Dibenzodioxins 0-4 Harvey rat sarcoma virus oncogene Mus musculus 33-39 1404241-7 1992 TCDD evoked increases in hepatic p21ras protein of C57BL/6J mice were accompanied by the increase in the specific binding of GTP to hepatic plasma membranes. Guanosine Triphosphate 125-128 Harvey rat sarcoma virus oncogene Mus musculus 33-39 1617670-8 1992 We also found that 2 nonmelanoma tumors induced by dimethylbenz[a]anthracene + UV + croton oil contained an A----T transversion at Ha-ras codon 61 position 2, which is characteristic of most dimethylbenz[a]anthracene-induced tumors. dimethylbenz 51-63 Harvey rat sarcoma virus oncogene Mus musculus 131-137 1617670-8 1992 We also found that 2 nonmelanoma tumors induced by dimethylbenz[a]anthracene + UV + croton oil contained an A----T transversion at Ha-ras codon 61 position 2, which is characteristic of most dimethylbenz[a]anthracene-induced tumors. anthracene 66-76 Harvey rat sarcoma virus oncogene Mus musculus 131-137 1617670-8 1992 We also found that 2 nonmelanoma tumors induced by dimethylbenz[a]anthracene + UV + croton oil contained an A----T transversion at Ha-ras codon 61 position 2, which is characteristic of most dimethylbenz[a]anthracene-induced tumors. Croton Oil 84-94 Harvey rat sarcoma virus oncogene Mus musculus 131-137 1617670-8 1992 We also found that 2 nonmelanoma tumors induced by dimethylbenz[a]anthracene + UV + croton oil contained an A----T transversion at Ha-ras codon 61 position 2, which is characteristic of most dimethylbenz[a]anthracene-induced tumors. dimethylbenz 191-203 Harvey rat sarcoma virus oncogene Mus musculus 131-137 1617670-8 1992 We also found that 2 nonmelanoma tumors induced by dimethylbenz[a]anthracene + UV + croton oil contained an A----T transversion at Ha-ras codon 61 position 2, which is characteristic of most dimethylbenz[a]anthracene-induced tumors. anthracene 206-216 Harvey rat sarcoma virus oncogene Mus musculus 131-137 1638698-0 1992 Temporal changes in the mutant frequency and mutation spectra of the 61st codon of the H-ras oncogene following exposure of B6C3F1 mice to N-nitrosodiethylamine (DEN). Diethylnitrosamine 162-165 Harvey rat sarcoma virus oncogene Mus musculus 87-92 1638698-9 1992 These data indicate that DEN-induced mutations at the 61st codon of the mouse H-ras oncogene (i) are an infrequent event, (ii) have different frequencies at the 38 and 65 week timepoints and (iii) are different from the types of mutations seen in spontaneous lesions. Diethylnitrosamine 25-28 Harvey rat sarcoma virus oncogene Mus musculus 78-83 1551113-0 1992 Induction of mutation of a synthetic c-Ha-ras gene containing hypoxanthine. Hypoxanthine 62-74 Harvey rat sarcoma virus oncogene Mus musculus 37-45 1551113-1 1992 The second base of codon 61 of a synthetic c-Ha-ras gene was replaced with a hypoxanthine residue in a site-specific manner. Hypoxanthine 77-89 Harvey rat sarcoma virus oncogene Mus musculus 43-51 1464157-9 1992 We have previously reported that limonene, perillic acid, and dihydroperillic acid inhibit the posttranslational isoprenylation of p21ras and other 21- to 26-kDa cell-growth-associated proteins in NIH3T3 cells and in mammary epithelial cells. Limonene 33-41 Harvey rat sarcoma virus oncogene Mus musculus 131-137 1547824-6 1992 In T lymphocytes, activation of protein kinase C (PKC) with phorbol esters is sufficient to stimulate p21ras. Phorbol Esters 60-74 Harvey rat sarcoma virus oncogene Mus musculus 102-108 1312398-0 1992 The tumour promoters dieldrin and phenobarbital increase the frequency of c-Ha-ras wild-type, but not of c-Ha-ras mutated focal liver lesions in male C3H/He mice. Dieldrin 21-29 Harvey rat sarcoma virus oncogene Mus musculus 74-82 1312398-0 1992 The tumour promoters dieldrin and phenobarbital increase the frequency of c-Ha-ras wild-type, but not of c-Ha-ras mutated focal liver lesions in male C3H/He mice. Phenobarbital 34-47 Harvey rat sarcoma virus oncogene Mus musculus 74-82 1372356-5 1992 Furthermore, it has been demonstrated that there is a direct correlation between the two markers, as blocking antibody to p21ras cancels the ability of gamma-IFN and 5-AzaC to induce class II antigens. Azacitidine 166-172 Harvey rat sarcoma virus oncogene Mus musculus 122-128 1540938-0 1992 Detection of a Ha-ras point mutation by polymerase chain reaction-single strand conformation polymorphism analysis in 2-amino-3,4-dimethylimidazo[4,5-f]quinoline-induced mouse forestomach tumors. 2-amino-3,4-dimethylimidazo(4,5-f)quinoline 118-161 Harvey rat sarcoma virus oncogene Mus musculus 15-21 1540938-1 1992 Ha-ras activation in forestomach squamous cell carcinomas of CDF1 mice induced by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), one of the heterocyclic amines isolated from broiled sardine was analyzed. 2-amino-3,4-dimethylimidazo(4,5-f)quinoline 82-125 Harvey rat sarcoma virus oncogene Mus musculus 0-6 1540938-1 1992 Ha-ras activation in forestomach squamous cell carcinomas of CDF1 mice induced by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), one of the heterocyclic amines isolated from broiled sardine was analyzed. 2-amino-3,4-dimethylimidazo(4,5-f)quinoline 127-131 Harvey rat sarcoma virus oncogene Mus musculus 0-6 1540938-1 1992 Ha-ras activation in forestomach squamous cell carcinomas of CDF1 mice induced by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), one of the heterocyclic amines isolated from broiled sardine was analyzed. Amines 158-164 Harvey rat sarcoma virus oncogene Mus musculus 0-6 1735612-10 1992 In contrast, small but statistically significant reductions in NAb binding were observed following v-H-ras transformation of NIH 3T3 cells or v-src transformation of 10T 1/2. nab 63-66 Harvey rat sarcoma virus oncogene Mus musculus 101-106 1464157-9 1992 We have previously reported that limonene, perillic acid, and dihydroperillic acid inhibit the posttranslational isoprenylation of p21ras and other 21- to 26-kDa cell-growth-associated proteins in NIH3T3 cells and in mammary epithelial cells. perillic acid 43-56 Harvey rat sarcoma virus oncogene Mus musculus 131-137 1733568-5 1992 A to T mutation at the second base of codon 61 of the Ha-ras oncogene was found in skin tumors of DMBA-exposed mice, but not in tumors induced by TPA without initiation. 9,10-Dimethyl-1,2-benzanthracene 98-102 Harvey rat sarcoma virus oncogene Mus musculus 54-60 1464157-9 1992 We have previously reported that limonene, perillic acid, and dihydroperillic acid inhibit the posttranslational isoprenylation of p21ras and other 21- to 26-kDa cell-growth-associated proteins in NIH3T3 cells and in mammary epithelial cells. dihydroperillic acid 62-82 Harvey rat sarcoma virus oncogene Mus musculus 131-137 1497802-1 1992 The presence of an activating mutation in the Ha-ras gene in hamster cheek pouch tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) complete carcinogenesis was investigated. 7,12-dimethylbenz[a 99-118 Harvey rat sarcoma virus oncogene Mus musculus 46-52 1323970-0 1992 Role of mutations at codon 61 of the c-Ha-ras gene during diethylnitrosamine-induced hepatocarcinogenesis in C3H/He mice. Diethylnitrosamine 58-76 Harvey rat sarcoma virus oncogene Mus musculus 37-45 1497802-1 1992 The presence of an activating mutation in the Ha-ras gene in hamster cheek pouch tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) complete carcinogenesis was investigated. anthracene 119-129 Harvey rat sarcoma virus oncogene Mus musculus 46-52 1497802-1 1992 The presence of an activating mutation in the Ha-ras gene in hamster cheek pouch tumors induced by 7,12-dimethylbenz[a]anthracene (DMBA) complete carcinogenesis was investigated. 9,10-Dimethyl-1,2-benzanthracene 131-135 Harvey rat sarcoma virus oncogene Mus musculus 46-52 1497802-5 1992 Paraffin sections of 11 squamous cell carcinomas of the cheek pouch were used to detect mutated Ha-ras alleles. Paraffin 0-8 Harvey rat sarcoma virus oncogene Mus musculus 96-102 1503641-1 1992 During two-stage mouse skin tumorigenesis, the mouse c-Ha-ras oncogene undergoes activation by point mutation after initiation with polycyclic aromatic hydrocarbons. Polycyclic Aromatic Hydrocarbons 132-164 Harvey rat sarcoma virus oncogene Mus musculus 53-61 1503641-2 1992 Furthermore, initiated epidermal cells containing an activated Ha-ras oncogene have been shown to be resistant to calcium-induced terminal differentiation. Calcium 114-121 Harvey rat sarcoma virus oncogene Mus musculus 63-69 1503641-6 1992 Steady-state levels of Ha-ras mRNA remained unchanged in primary cultures of normal adult epidermal cells during calcium-induced differentiation, whereas steady-state levels of Ha-ras transcripts decreased during calcium-induced differentiation in primary newborn epidermal cells. Calcium 213-220 Harvey rat sarcoma virus oncogene Mus musculus 177-183 1503644-0 1992 Analysis of point mutations in murine c-Ha-ras of skin tumors initiated with dibenz[a,j]anthracene and derivatives. dibenzo(aj)anthracene 77-98 Harvey rat sarcoma virus oncogene Mus musculus 38-46 1503644-1 1992 This study was designed to evaluate the point mutations in the murine c-Ha-ras gene of skin papillomas induced by initiation with dibenz[a,j]anthracene (DB[a,j]A), its bay-region anti-diol epoxide ((+/-)anti-DB[a,j]A-DE), and a 7,14-dimethyl analogue (7,14-diMeDB[a,j]A). dibenz 130-136 Harvey rat sarcoma virus oncogene Mus musculus 70-78 1503644-1 1992 This study was designed to evaluate the point mutations in the murine c-Ha-ras gene of skin papillomas induced by initiation with dibenz[a,j]anthracene (DB[a,j]A), its bay-region anti-diol epoxide ((+/-)anti-DB[a,j]A-DE), and a 7,14-dimethyl analogue (7,14-diMeDB[a,j]A). anthracene 141-151 Harvey rat sarcoma virus oncogene Mus musculus 70-78 1503644-1 1992 This study was designed to evaluate the point mutations in the murine c-Ha-ras gene of skin papillomas induced by initiation with dibenz[a,j]anthracene (DB[a,j]A), its bay-region anti-diol epoxide ((+/-)anti-DB[a,j]A-DE), and a 7,14-dimethyl analogue (7,14-diMeDB[a,j]A). diol epoxide 184-196 Harvey rat sarcoma virus oncogene Mus musculus 70-78 1503644-1 1992 This study was designed to evaluate the point mutations in the murine c-Ha-ras gene of skin papillomas induced by initiation with dibenz[a,j]anthracene (DB[a,j]A), its bay-region anti-diol epoxide ((+/-)anti-DB[a,j]A-DE), and a 7,14-dimethyl analogue (7,14-diMeDB[a,j]A). 7,14-dimethyl 228-241 Harvey rat sarcoma virus oncogene Mus musculus 70-78 1289809-1 1992 In order to investigate whether several DNA lesions (O6-methylguanine, 8-hydroxyguanine, xanthine, an abasic site analogue and hypoxanthine) activate a c-Ha-ras gene and to determine the type of mutations induced by the DNA lesions, they were introduced into a synthetic c-Ha-ras gene by DNA cassette mutagenesis techniques. O-(6)-methylguanine 53-69 Harvey rat sarcoma virus oncogene Mus musculus 152-160 1747936-0 1991 Analysis of the Ha-ras oncogene in C3H/He mouse liver tumours derived spontaneously or induced with diethylnitrosamine or phenobarbitone. Diethylnitrosamine 100-118 Harvey rat sarcoma virus oncogene Mus musculus 16-22 1289809-1 1992 In order to investigate whether several DNA lesions (O6-methylguanine, 8-hydroxyguanine, xanthine, an abasic site analogue and hypoxanthine) activate a c-Ha-ras gene and to determine the type of mutations induced by the DNA lesions, they were introduced into a synthetic c-Ha-ras gene by DNA cassette mutagenesis techniques. 8-hydroxyguanine 71-87 Harvey rat sarcoma virus oncogene Mus musculus 152-160 1289809-1 1992 In order to investigate whether several DNA lesions (O6-methylguanine, 8-hydroxyguanine, xanthine, an abasic site analogue and hypoxanthine) activate a c-Ha-ras gene and to determine the type of mutations induced by the DNA lesions, they were introduced into a synthetic c-Ha-ras gene by DNA cassette mutagenesis techniques. Xanthine 89-97 Harvey rat sarcoma virus oncogene Mus musculus 152-160 1289809-1 1992 In order to investigate whether several DNA lesions (O6-methylguanine, 8-hydroxyguanine, xanthine, an abasic site analogue and hypoxanthine) activate a c-Ha-ras gene and to determine the type of mutations induced by the DNA lesions, they were introduced into a synthetic c-Ha-ras gene by DNA cassette mutagenesis techniques. Hypoxanthine 127-139 Harvey rat sarcoma virus oncogene Mus musculus 152-160 1747936-0 1991 Analysis of the Ha-ras oncogene in C3H/He mouse liver tumours derived spontaneously or induced with diethylnitrosamine or phenobarbitone. Phenobarbital 122-136 Harvey rat sarcoma virus oncogene Mus musculus 16-22 1747936-1 1991 In a study of the mechanisms involved in the induction of tumours by chemicals, the Ha-ras oncogene was analysed in liver tumours induced by the genotoxic carcinogen diethylnitrosamine (DEN), or the non-genotoxic agent phenobarbitone (PB) in C3H/He mice. Diethylnitrosamine 166-184 Harvey rat sarcoma virus oncogene Mus musculus 84-90 1747936-1 1991 In a study of the mechanisms involved in the induction of tumours by chemicals, the Ha-ras oncogene was analysed in liver tumours induced by the genotoxic carcinogen diethylnitrosamine (DEN), or the non-genotoxic agent phenobarbitone (PB) in C3H/He mice. Diethylnitrosamine 186-189 Harvey rat sarcoma virus oncogene Mus musculus 84-90 1747936-1 1991 In a study of the mechanisms involved in the induction of tumours by chemicals, the Ha-ras oncogene was analysed in liver tumours induced by the genotoxic carcinogen diethylnitrosamine (DEN), or the non-genotoxic agent phenobarbitone (PB) in C3H/He mice. Phenobarbital 219-233 Harvey rat sarcoma virus oncogene Mus musculus 84-90 1747936-1 1991 In a study of the mechanisms involved in the induction of tumours by chemicals, the Ha-ras oncogene was analysed in liver tumours induced by the genotoxic carcinogen diethylnitrosamine (DEN), or the non-genotoxic agent phenobarbitone (PB) in C3H/He mice. Phenobarbital 235-237 Harvey rat sarcoma virus oncogene Mus musculus 84-90 1747936-6 1991 Low and variable expression of the Ha-ras gene was detected in all liver tissues with moderately raised levels (175-200%) in spontaneous, DEN and PB-induced tumours as compared to normal liver tissue. Diethylnitrosamine 138-141 Harvey rat sarcoma virus oncogene Mus musculus 35-41 1747936-6 1991 Low and variable expression of the Ha-ras gene was detected in all liver tissues with moderately raised levels (175-200%) in spontaneous, DEN and PB-induced tumours as compared to normal liver tissue. Phenobarbital 146-148 Harvey rat sarcoma virus oncogene Mus musculus 35-41 1747936-8 1991 The frequency of the Ha-ras mutation at codon 61 in DEN-induced tumours is greater than in spontaneously arising tumours. Diethylnitrosamine 52-55 Harvey rat sarcoma virus oncogene Mus musculus 21-27 1934268-2 1991 After initiation with dimethylbenzanthracene (DMBA) in vivo, greater than 90% of the papillomas arising show the same A:T----T:A transversion at codon 61 of the H-ras gene, presumed to be the initiating event. 9,10-Dimethyl-1,2-benzanthracene 22-44 Harvey rat sarcoma virus oncogene Mus musculus 161-166 1657373-0 1991 Expression of the c-Ha-ras oncogene in mouse NIH 3T3 cells induces resistance to cisplatin. Cisplatin 81-90 Harvey rat sarcoma virus oncogene Mus musculus 18-26 1657373-1 1991 The effect of expression of the c-Ha-ras oncogene on cisplatin (DDP) sensitivity was examined in murine NIH 3T3 cells transfected with the dexamethasone (DEX)-inducible mouse mammary tumor virus promoter linked to an activated c-Ha-ras gene [LTR H-ras(A) cells]. Cisplatin 53-62 Harvey rat sarcoma virus oncogene Mus musculus 32-40 1657373-7 1991 We conclude that DEX-induced overexpression of a mutant c-Ha-ras gene confers DDP resistance and that this resistance is associated with an impairment of cellular drug accumulation and an increase in metallothionein content. Dexamethasone 17-20 Harvey rat sarcoma virus oncogene Mus musculus 56-64 1658621-6 1991 This induction could be suppressed by cotransfection of two inhibitory mutant ras genes, H-ras(Asn-17) or H-ras(Leu-61,Ser-186). Asparagine 95-98 Harvey rat sarcoma virus oncogene Mus musculus 89-94 1658621-6 1991 This induction could be suppressed by cotransfection of two inhibitory mutant ras genes, H-ras(Asn-17) or H-ras(Leu-61,Ser-186). Leucine 112-115 Harvey rat sarcoma virus oncogene Mus musculus 106-111 1658621-6 1991 This induction could be suppressed by cotransfection of two inhibitory mutant ras genes, H-ras(Asn-17) or H-ras(Leu-61,Ser-186). Serine 119-122 Harvey rat sarcoma virus oncogene Mus musculus 106-111 1657633-0 1991 Interference of Ha-ras with inositol trisphosphate-mediated Ca(2+)-release. inositol 1,2,3-trisphosphate 28-50 Harvey rat sarcoma virus oncogene Mus musculus 16-22 1657633-1 1991 Expression of a transforming Ha-ras by dexamethasone in NIH3T3 cells transfected with a glucocorticoid-inducible Ha-ras construct results in a rapid desensitization of the intracellular Ca(2+)-mobilizing system to bombesin. Dexamethasone 39-52 Harvey rat sarcoma virus oncogene Mus musculus 29-35 1657633-1 1991 Expression of a transforming Ha-ras by dexamethasone in NIH3T3 cells transfected with a glucocorticoid-inducible Ha-ras construct results in a rapid desensitization of the intracellular Ca(2+)-mobilizing system to bombesin. Dexamethasone 39-52 Harvey rat sarcoma virus oncogene Mus musculus 113-119 1657633-2 1991 This effect precedes the down-modulation of inositol trisphosphate (IP3) formation by several hours and is, therefore, not explained by an uncoupling of phosphoinositidase C. It is demonstrated that expression of Ha-ras attenuates the Ca(2+)-release by IP3 in permeabilized cells. inositol 1,2,3-trisphosphate 44-66 Harvey rat sarcoma virus oncogene Mus musculus 213-219 1657633-2 1991 This effect precedes the down-modulation of inositol trisphosphate (IP3) formation by several hours and is, therefore, not explained by an uncoupling of phosphoinositidase C. It is demonstrated that expression of Ha-ras attenuates the Ca(2+)-release by IP3 in permeabilized cells. Inositol 1,4,5-Trisphosphate 68-71 Harvey rat sarcoma virus oncogene Mus musculus 213-219 1657633-2 1991 This effect precedes the down-modulation of inositol trisphosphate (IP3) formation by several hours and is, therefore, not explained by an uncoupling of phosphoinositidase C. It is demonstrated that expression of Ha-ras attenuates the Ca(2+)-release by IP3 in permeabilized cells. Inositol 1,4,5-Trisphosphate 253-256 Harvey rat sarcoma virus oncogene Mus musculus 213-219 1657633-3 1991 The IP3 concentration required for half-maximal Ca(2+)-release is doubled in Ha-ras expressing cells. Inositol 1,4,5-Trisphosphate 4-7 Harvey rat sarcoma virus oncogene Mus musculus 77-83 1657633-4 1991 Maximal Ca(2+)-release which is obtained with 2 microM IP3 in control cells requires 10 microM IP3 in cells expressing Ha-ras. Inositol 1,4,5-Trisphosphate 95-98 Harvey rat sarcoma virus oncogene Mus musculus 119-125 1657633-6 1991 The results indicate that the Ha-ras mediated desensitization of the Ca(2+)-releasing system to bombesin is--at least in part--caused by a decrease in the affinity of the IP3 receptor to inositol trisphosphate. inositol 1,2,3-trisphosphate 187-209 Harvey rat sarcoma virus oncogene Mus musculus 30-36 1934268-2 1991 After initiation with dimethylbenzanthracene (DMBA) in vivo, greater than 90% of the papillomas arising show the same A:T----T:A transversion at codon 61 of the H-ras gene, presumed to be the initiating event. 9,10-Dimethyl-1,2-benzanthracene 46-50 Harvey rat sarcoma virus oncogene Mus musculus 161-166 1756258-9 1991 Our results demonstrate that i) persistent expression of normal and mutant c-Ha-RAS can bring about tumorigenic transformation of mouse mammary epithelial cells; and ii) alteration in estradiol metabolism and acquisition of anchorage-independent growth precede the emergence of a tumorigenic phenotype. Estradiol 184-193 Harvey rat sarcoma virus oncogene Mus musculus 75-83 1938604-0 1991 Transforming activity of a synthetic c-Ha-ras gene containing O6-methylguanine in codon 12. O-(6)-methylguanine 62-78 Harvey rat sarcoma virus oncogene Mus musculus 37-45 1938604-1 1991 A mutagenic DNA-adduct, O6-methylguanine, was introduced into codon 12 of the synthetic c-Ha-ras gene by cassette mutagenesis. O-(6)-methylguanine 24-40 Harvey rat sarcoma virus oncogene Mus musculus 88-96 1679532-2 1991 When c-H-ras expression was induced with heavy metal ions there was a marked reduction in the expression of two glycosylphosphatidylinositol (GPI) anchored proteins, TAP/Ly-6A.2 and Thy-1, on the plasma membrane of the transformed cells. Metals 47-52 Harvey rat sarcoma virus oncogene Mus musculus 5-12 1884371-0 1991 Activating mutations at codon 61 of the c-Ha-ras gene in thin-tissue sections of tumors induced by aristolochic acid in rats and mice. aristolochic acid I 99-116 Harvey rat sarcoma virus oncogene Mus musculus 40-48 1679532-2 1991 When c-H-ras expression was induced with heavy metal ions there was a marked reduction in the expression of two glycosylphosphatidylinositol (GPI) anchored proteins, TAP/Ly-6A.2 and Thy-1, on the plasma membrane of the transformed cells. Glycosylphosphatidylinositols 112-140 Harvey rat sarcoma virus oncogene Mus musculus 5-12 1679532-2 1991 When c-H-ras expression was induced with heavy metal ions there was a marked reduction in the expression of two glycosylphosphatidylinositol (GPI) anchored proteins, TAP/Ly-6A.2 and Thy-1, on the plasma membrane of the transformed cells. Glycosylphosphatidylinositols 142-145 Harvey rat sarcoma virus oncogene Mus musculus 5-12 2072908-2 1991 One, [Asn-17]Ras, had a substitution in the putative Mg(2+)-binding site of Ha-Ras. magnesium ion 53-59 Harvey rat sarcoma virus oncogene Mus musculus 76-82 1663450-3 1991 DNA samples isolated from tumors induced by dimethylbenz[alpha]anthracene and each of the okadaic acid class tumor promoters had the same mutation at the second nucleotide of codon 61 (CAA to CTA) in the c-H-ras gene. dimethylbenz[alpha]anthracene 44-73 Harvey rat sarcoma virus oncogene Mus musculus 204-211 1647307-1 1991 Granulosa cell lines, transformed by SV40 T-antigen and Ha-ras oncogene, have recently been established that can produce progesterone at levels comparable to those of highly differentiated cultures of primary granulosa cells (1-4). Progesterone 121-133 Harvey rat sarcoma virus oncogene Mus musculus 56-62 2049075-0 1991 Phorbol ester and bryostatin differentially regulate the hydrolysis of phosphatidylethanolamine in Ha-ras- and raf-oncogene-transformed NIH 3T3 cells. Phorbol Esters 0-13 Harvey rat sarcoma virus oncogene Mus musculus 99-105 1712678-0 1991 Induction of glutathione content in murine melanocytes after transformation with c-H-ras oncogene. Glutathione 13-24 Harvey rat sarcoma virus oncogene Mus musculus 81-88 2049075-0 1991 Phorbol ester and bryostatin differentially regulate the hydrolysis of phosphatidylethanolamine in Ha-ras- and raf-oncogene-transformed NIH 3T3 cells. Bryostatins 18-28 Harvey rat sarcoma virus oncogene Mus musculus 99-105 2049075-0 1991 Phorbol ester and bryostatin differentially regulate the hydrolysis of phosphatidylethanolamine in Ha-ras- and raf-oncogene-transformed NIH 3T3 cells. phosphatidylethanolamine 71-95 Harvey rat sarcoma virus oncogene Mus musculus 99-105 1663450-3 1991 DNA samples isolated from tumors induced by dimethylbenz[alpha]anthracene and each of the okadaic acid class tumor promoters had the same mutation at the second nucleotide of codon 61 (CAA to CTA) in the c-H-ras gene. Okadaic Acid 90-102 Harvey rat sarcoma virus oncogene Mus musculus 204-211 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. gdp-gtp 74-81 Harvey rat sarcoma virus oncogene Mus musculus 16-22 2022640-1 1991 The expression of Ha-ras in quiescent NIH3T3 cells carrying a glucocorticoid-inducible human Ha-ras gene (Val-Gly mutation at codon 12) stimulates total 86Rb+ influx. Valine 106-109 Harvey rat sarcoma virus oncogene Mus musculus 18-24 2022640-1 1991 The expression of Ha-ras in quiescent NIH3T3 cells carrying a glucocorticoid-inducible human Ha-ras gene (Val-Gly mutation at codon 12) stimulates total 86Rb+ influx. Valine 106-109 Harvey rat sarcoma virus oncogene Mus musculus 93-99 2022640-1 1991 The expression of Ha-ras in quiescent NIH3T3 cells carrying a glucocorticoid-inducible human Ha-ras gene (Val-Gly mutation at codon 12) stimulates total 86Rb+ influx. Glycine 110-113 Harvey rat sarcoma virus oncogene Mus musculus 18-24 2022640-1 1991 The expression of Ha-ras in quiescent NIH3T3 cells carrying a glucocorticoid-inducible human Ha-ras gene (Val-Gly mutation at codon 12) stimulates total 86Rb+ influx. Glycine 110-113 Harvey rat sarcoma virus oncogene Mus musculus 93-99 2022640-5 1991 Overexpression of the Ha-ras proto-oncogene causes only a marginal increase in total 86Rb+ uptake. Rubidium-86 85-90 Harvey rat sarcoma virus oncogene Mus musculus 22-28 2022640-6 1991 The stimulation of the furosemide-sensitive influx by Ha-ras is paralleled by an increase in mean cell volume which can be inhibited by furosemide. Furosemide 23-33 Harvey rat sarcoma virus oncogene Mus musculus 54-60 2022640-6 1991 The stimulation of the furosemide-sensitive influx by Ha-ras is paralleled by an increase in mean cell volume which can be inhibited by furosemide. Furosemide 136-146 Harvey rat sarcoma virus oncogene Mus musculus 54-60 2022640-8 1991 Furosemide inhibits the mitogenic response after expression of Ha-ras or addition of bombesin. Furosemide 0-10 Harvey rat sarcoma virus oncogene Mus musculus 63-69 2022640-9 1991 Both the Ha-ras and the bombesin-induced stimulation of the furosemide-sensitive Rb+ transport can be blocked by protein kinase C depletion or the protein kinase C inhibitor staurosporine. Furosemide 60-70 Harvey rat sarcoma virus oncogene Mus musculus 9-15 2022640-9 1991 Both the Ha-ras and the bombesin-induced stimulation of the furosemide-sensitive Rb+ transport can be blocked by protein kinase C depletion or the protein kinase C inhibitor staurosporine. Staurosporine 174-187 Harvey rat sarcoma virus oncogene Mus musculus 9-15 2022640-10 1991 In contrast to bombesin-induced phosphatidylinositol-4,5-bisphosphate hydrolysis which is down-modulated by Ha-ras, the stimulation of the furosemide-sensitive Rb+ influx by bombesin is elevated in Ha-ras-expressing cells. Furosemide 139-149 Harvey rat sarcoma virus oncogene Mus musculus 198-204 2043425-1 1991 Post-translational modification of oncogenic p21ras proteins with farnesyl, a lipid intermediate in cholesterol biosynthesis, is required for p21ras membrane association and for the ability of p21ras to transform cultured cells. FARNESYL 66-74 Harvey rat sarcoma virus oncogene Mus musculus 45-51 2043425-1 1991 Post-translational modification of oncogenic p21ras proteins with farnesyl, a lipid intermediate in cholesterol biosynthesis, is required for p21ras membrane association and for the ability of p21ras to transform cultured cells. FARNESYL 66-74 Harvey rat sarcoma virus oncogene Mus musculus 142-148 2043425-1 1991 Post-translational modification of oncogenic p21ras proteins with farnesyl, a lipid intermediate in cholesterol biosynthesis, is required for p21ras membrane association and for the ability of p21ras to transform cultured cells. FARNESYL 66-74 Harvey rat sarcoma virus oncogene Mus musculus 142-148 2043425-1 1991 Post-translational modification of oncogenic p21ras proteins with farnesyl, a lipid intermediate in cholesterol biosynthesis, is required for p21ras membrane association and for the ability of p21ras to transform cultured cells. Cholesterol 100-111 Harvey rat sarcoma virus oncogene Mus musculus 45-51 2043425-1 1991 Post-translational modification of oncogenic p21ras proteins with farnesyl, a lipid intermediate in cholesterol biosynthesis, is required for p21ras membrane association and for the ability of p21ras to transform cultured cells. Cholesterol 100-111 Harvey rat sarcoma virus oncogene Mus musculus 142-148 2043425-1 1991 Post-translational modification of oncogenic p21ras proteins with farnesyl, a lipid intermediate in cholesterol biosynthesis, is required for p21ras membrane association and for the ability of p21ras to transform cultured cells. Cholesterol 100-111 Harvey rat sarcoma virus oncogene Mus musculus 142-148 2043425-4 1991 Immunoprecipitation studies with transformed EJ cells showed that lovastatin (1-100 microM) inhibited p21ras membrane association in a concentration-dependent manner and that a 10 microM concentration reduced the amount of p21ras bound to the membrane by 50%. Lovastatin 66-76 Harvey rat sarcoma virus oncogene Mus musculus 102-108 2043425-4 1991 Immunoprecipitation studies with transformed EJ cells showed that lovastatin (1-100 microM) inhibited p21ras membrane association in a concentration-dependent manner and that a 10 microM concentration reduced the amount of p21ras bound to the membrane by 50%. Lovastatin 66-76 Harvey rat sarcoma virus oncogene Mus musculus 223-229 2043425-5 1991 Lovastatin also inhibited EJ cell growth in a concentration range that closely paralleled that required for inhibition of p21ras membrane association. Lovastatin 0-10 Harvey rat sarcoma virus oncogene Mus musculus 122-128 2043425-7 1991 Western blotting studies showed that lovastatin (50 mg/kg) was also able to inhibit p21ras membrane association in EJ tumors implanted s.c. in nude mice. Lovastatin 37-47 Harvey rat sarcoma virus oncogene Mus musculus 84-90 2043425-8 1991 These results demonstrate that lovastatin, an inhibitor of cholesterol biosynthesis, inhibited in vivo tumor growth of H-ras oncogene transformed cells. Lovastatin 31-41 Harvey rat sarcoma virus oncogene Mus musculus 119-124 2043425-8 1991 These results demonstrate that lovastatin, an inhibitor of cholesterol biosynthesis, inhibited in vivo tumor growth of H-ras oncogene transformed cells. Cholesterol 59-70 Harvey rat sarcoma virus oncogene Mus musculus 119-124 2043425-9 1991 The results also suggest that inhibition of p21ras membrane association, an essential step in ras oncogene neoplastic transformation, is one mechanism by which lovastatin may express its antitumor activity. Lovastatin 160-170 Harvey rat sarcoma virus oncogene Mus musculus 44-50 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Diphosphate 74-77 Harvey rat sarcoma virus oncogene Mus musculus 16-22 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Diphosphate 74-77 Harvey rat sarcoma virus oncogene Mus musculus 89-95 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Diphosphate 74-77 Harvey rat sarcoma virus oncogene Mus musculus 89-95 2017178-3 1991 A GTPase-activating protein (GAP) mediates the inactivation of p21ras by facilitating the conversion of the active p21ras-GTP to the inactive p21ras-GDP. Guanosine Triphosphate 2-5 Harvey rat sarcoma virus oncogene Mus musculus 63-69 2017178-3 1991 A GTPase-activating protein (GAP) mediates the inactivation of p21ras by facilitating the conversion of the active p21ras-GTP to the inactive p21ras-GDP. Guanosine Triphosphate 2-5 Harvey rat sarcoma virus oncogene Mus musculus 115-121 2017178-3 1991 A GTPase-activating protein (GAP) mediates the inactivation of p21ras by facilitating the conversion of the active p21ras-GTP to the inactive p21ras-GDP. Guanosine Diphosphate 149-152 Harvey rat sarcoma virus oncogene Mus musculus 63-69 2017178-3 1991 A GTPase-activating protein (GAP) mediates the inactivation of p21ras by facilitating the conversion of the active p21ras-GTP to the inactive p21ras-GDP. Guanosine Diphosphate 149-152 Harvey rat sarcoma virus oncogene Mus musculus 115-121 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. gdp-gtp 74-81 Harvey rat sarcoma virus oncogene Mus musculus 89-95 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. gdp-gtp 74-81 Harvey rat sarcoma virus oncogene Mus musculus 89-95 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Triphosphate 78-81 Harvey rat sarcoma virus oncogene Mus musculus 16-22 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Triphosphate 78-81 Harvey rat sarcoma virus oncogene Mus musculus 89-95 2017178-2 1991 The activity of p21ras itself is regulated by its cyclic association with GDP-GTP, where p21ras-GTP is the active form and p21ras-GDP is the inactive form. Guanosine Triphosphate 78-81 Harvey rat sarcoma virus oncogene Mus musculus 89-95 1899909-4 1991 COOH-terminal processing and membrane association of Rap1A were studied by constructing and expressing a chimeric protein (composed of residues 1 to 110 of an H-Ras activated by a Leu-61 mutation attached to residues 111 to 184 of Rap1A) in NIH 3T3 cells and a full-length human Rap1A protein in a baculovirus-Sf9 insect cell system. Leucine 180-183 Harvey rat sarcoma virus oncogene Mus musculus 159-164 1909909-1 1991 The mutation was revealed with substitution of A for T in the second position of the 61 Ha-ras oncogene codon in the DNA of 31 skin tumours (26 papillomas and 5 carcinomas) and in 23 mice treated with 12-O-tetradecanoyl phorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 201-238 Harvey rat sarcoma virus oncogene Mus musculus 88-94 1846354-4 1991 A maximum is obtained after 6 h. Bombesin-induced elevation of inositol 1,4,5-trisphosphate formation is also depressed in cells expressing Ha-ras. Inositol 1,4,5-Trisphosphate 63-91 Harvey rat sarcoma virus oncogene Mus musculus 140-146 1992343-8 1991 Inhibition by antisense RNA or craf301 blocked proliferation and transformation by Ki- and Ha-ras oncogenes. craf301 31-38 Harvey rat sarcoma virus oncogene Mus musculus 91-97 2000226-1 1991 By selective oligonucleotide hybridization of polymerase chain reaction (PCR) amplified tumor DNAs we have analysed the incidence of mutations at codon 61 of the Ha-ras gene in 42 liver tumors spontaneously developed in Balb/c, C3Hf and B6C3 male mice, and in 79 liver tumors induced by the chemical carcinogens diethylnitrosamine (NDEA) and urethan in B6C3 and B6C male and female mice. c3hf 228-232 Harvey rat sarcoma virus oncogene Mus musculus 162-168 2000226-1 1991 By selective oligonucleotide hybridization of polymerase chain reaction (PCR) amplified tumor DNAs we have analysed the incidence of mutations at codon 61 of the Ha-ras gene in 42 liver tumors spontaneously developed in Balb/c, C3Hf and B6C3 male mice, and in 79 liver tumors induced by the chemical carcinogens diethylnitrosamine (NDEA) and urethan in B6C3 and B6C male and female mice. Diethylnitrosamine 312-330 Harvey rat sarcoma virus oncogene Mus musculus 162-168 2000226-1 1991 By selective oligonucleotide hybridization of polymerase chain reaction (PCR) amplified tumor DNAs we have analysed the incidence of mutations at codon 61 of the Ha-ras gene in 42 liver tumors spontaneously developed in Balb/c, C3Hf and B6C3 male mice, and in 79 liver tumors induced by the chemical carcinogens diethylnitrosamine (NDEA) and urethan in B6C3 and B6C male and female mice. Diethylnitrosamine 332-336 Harvey rat sarcoma virus oncogene Mus musculus 162-168 2000226-1 1991 By selective oligonucleotide hybridization of polymerase chain reaction (PCR) amplified tumor DNAs we have analysed the incidence of mutations at codon 61 of the Ha-ras gene in 42 liver tumors spontaneously developed in Balb/c, C3Hf and B6C3 male mice, and in 79 liver tumors induced by the chemical carcinogens diethylnitrosamine (NDEA) and urethan in B6C3 and B6C male and female mice. Urethane 342-349 Harvey rat sarcoma virus oncogene Mus musculus 162-168 2000226-2 1991 The incidence of Ha-ras gene mutations in both spontaneously developed and urethan-induced liver tumors was 50%-63% in mice genetically susceptible to hepatocarcinogenesis (C3Hf, B6C3) and 7%-9% in mice genetically resistant (Balb/c, B6C). Urethane 75-82 Harvey rat sarcoma virus oncogene Mus musculus 17-23 2000226-2 1991 The incidence of Ha-ras gene mutations in both spontaneously developed and urethan-induced liver tumors was 50%-63% in mice genetically susceptible to hepatocarcinogenesis (C3Hf, B6C3) and 7%-9% in mice genetically resistant (Balb/c, B6C). c3hf 173-177 Harvey rat sarcoma virus oncogene Mus musculus 17-23 2000226-4 1991 NDEA-induced tumors showed a low incidence of Ha-ras mutations in both the hybrid mice (3/18 and 1/13 in B6C3 and B6C male mice, respectively). Diethylnitrosamine 0-4 Harvey rat sarcoma virus oncogene Mus musculus 46-52 2000226-6 1991 Our results indicate that liver tumors induced by NDEA or urethan or spontaneously arisen have a different pattern of Ha-ras mutations at codon 61 and that these mutations constitute a rare molecular alteration in the pathogenesis of liver tumors in genetically resistant mice. Diethylnitrosamine 50-54 Harvey rat sarcoma virus oncogene Mus musculus 118-124 2000226-6 1991 Our results indicate that liver tumors induced by NDEA or urethan or spontaneously arisen have a different pattern of Ha-ras mutations at codon 61 and that these mutations constitute a rare molecular alteration in the pathogenesis of liver tumors in genetically resistant mice. Urethane 58-65 Harvey rat sarcoma virus oncogene Mus musculus 118-124 1824924-0 1991 Activation and cytotoxicity of 5"-deoxy-5-fluorouridine in c-H-ras transformed NIH 3T3 cells. doxifluridine 31-55 Harvey rat sarcoma virus oncogene Mus musculus 59-66 1824924-1 1991 Transformation of NIH 3T3 cells with c-H-ras has been demonstrated to result in significantly increased activation of 5"-deoxy-5-fluorouridine and significantly increased cytotoxicity in vitro as compared to non-transformed NIH 3T3. doxifluridine 118-142 Harvey rat sarcoma virus oncogene Mus musculus 37-44 1846354-7 1991 The inositol 1,4,5-trisphosphate-mediated release of intracellular Ca2+ is reduced in permeabilized cells expressing the Ha-ras oncogene. Inositol 1,4,5-Trisphosphate 4-32 Harvey rat sarcoma virus oncogene Mus musculus 121-127 1846354-8 1991 A depletion of intracellular Ca2+ stores by Ha-ras is unlikely since (i) the Ha-ras-induced growth factor-independent stimulation of inositol phosphate formation occurs several hours after reduction of the Ca2+ response and (ii) the Ca2+ load of intracellular nonmitochondrial Ca2+ stores was found to be unaffected by Ha-ras. Inositol Phosphates 133-151 Harvey rat sarcoma virus oncogene Mus musculus 77-83 1985780-3 1991 A factor reduction similar to that caused by activated Ha-ras was transiently obtained with 12-O-tetradecanoylphorbol-13-acetate in the PB-3c cells expressing normal c-Ha-ras. Tetradecanoylphorbol Acetate 92-128 Harvey rat sarcoma virus oncogene Mus musculus 55-61 1985780-4 1991 The analogous stimulation of protein kinase C (PKC) in PB-3c cells producing oncogenic Ha-ras led to an additional reduction of the IL-3 requirement during the first 24 h. In the absence of IL-3, the prolonged exposure of the cells to 12-O-tetradecanoylphorbol-13-acetate for 72 h resulted in a stimulation of growth when activated but not when normal Ha-ras was expressed. Tetradecanoylphorbol Acetate 235-271 Harvey rat sarcoma virus oncogene Mus musculus 87-93 1846354-8 1991 A depletion of intracellular Ca2+ stores by Ha-ras is unlikely since (i) the Ha-ras-induced growth factor-independent stimulation of inositol phosphate formation occurs several hours after reduction of the Ca2+ response and (ii) the Ca2+ load of intracellular nonmitochondrial Ca2+ stores was found to be unaffected by Ha-ras. Inositol Phosphates 133-151 Harvey rat sarcoma virus oncogene Mus musculus 77-83 1985780-6 1991 Upon 12-O-tetradecanoylphorbol-13-acetate treatment, a protracted down-regulation of the immunodetectable alpha-PKC as well as constitutively high levels of c-fos mRNA were observed when oncogenic Ha-ras was expressed. Tetradecanoylphorbol Acetate 5-41 Harvey rat sarcoma virus oncogene Mus musculus 197-203 1706723-0 1991 Effects of retinoic acid on NIH3T3 cell transformation by the H-ras oncogene. Tretinoin 11-24 Harvey rat sarcoma virus oncogene Mus musculus 62-67 2018350-0 1991 Correlation of growth capacity of cells in hard agarose with successful transfection by the activated c-Ha-ras oncogene and in vivo proliferative capacity at metastatic sites. Sepharose 48-55 Harvey rat sarcoma virus oncogene Mus musculus 102-110 2018350-1 1991 The purpose of this study was to determine whether the degree of anchorage-independent growth of rodent or human cells in increasing concentrations of agarose correlated with successful transfection of the cells with an activated c-Ha-ras oncogene and tumorigenicity in nude mice. Sepharose 151-158 Harvey rat sarcoma virus oncogene Mus musculus 230-238 1647646-0 1991 Effect of vitamin E succinate and a cAMP-stimulating agent on the expression of c-myc and N-myc and H-ras in murine neuroblastoma cells. alpha-Tocopherol 10-29 Harvey rat sarcoma virus oncogene Mus musculus 100-105 1647646-0 1991 Effect of vitamin E succinate and a cAMP-stimulating agent on the expression of c-myc and N-myc and H-ras in murine neuroblastoma cells. Cyclic AMP 36-40 Harvey rat sarcoma virus oncogene Mus musculus 100-105 1647646-1 1991 D-Alpha-tocopheryl succinate (vitamin E succinate) at a concentration of 11.3 microM inhibited growth and reduced the expression of c-myc, N-myc and H-ras specific mRNAs in murine neuroblastoma cells (NBP2) in culture. alpha-Tocopherol 0-28 Harvey rat sarcoma virus oncogene Mus musculus 149-154 1647646-1 1991 D-Alpha-tocopheryl succinate (vitamin E succinate) at a concentration of 11.3 microM inhibited growth and reduced the expression of c-myc, N-myc and H-ras specific mRNAs in murine neuroblastoma cells (NBP2) in culture. alpha-Tocopherol 30-49 Harvey rat sarcoma virus oncogene Mus musculus 149-154 1706723-1 1991 Exposure of NIH3T3 cells to retinoic acid resulted in a dose-dependent modulation of transformed focus formation after transfection with an activated H-ras oncogene. Tretinoin 28-41 Harvey rat sarcoma virus oncogene Mus musculus 150-155 1706723-6 1991 A transformed cell line containing H-ras underwent reversion of the transformed phenotype after 4 weeks of treatment with retinoic acid, as determined by alterations in cell morphology and anchorage-independent growth. Tretinoin 122-135 Harvey rat sarcoma virus oncogene Mus musculus 35-40 2219132-0 1990 Increased expression of c-myc and c-Ha-ras in dichloroacetate and trichloroacetate-induced liver tumors in B6C3F1 mice. Dichloroacetic Acid 46-61 Harvey rat sarcoma virus oncogene Mus musculus 34-42 1903226-0 1991 [Activation of the Ha-ras oncogene in tumors induced in mice by transplacental exposure to 7,12-dimethylbenz(a)anthracene]. 7,12-dimethylbenz(a 91-110 Harvey rat sarcoma virus oncogene Mus musculus 19-25 1903226-0 1991 [Activation of the Ha-ras oncogene in tumors induced in mice by transplacental exposure to 7,12-dimethylbenz(a)anthracene]. anthracene 111-121 Harvey rat sarcoma virus oncogene Mus musculus 19-25 1903226-1 1991 A study of tumors induced in mice by transplacental exposure to 7,12-dimethylbenz(a)anthracene (DMBA) alone or in combination with postnatal tissue-specific promotion showed skin and liver tumor development to be associated with cellular Ha-ras oncogene activation in a large percentage of cases. 7,12-dimethylbenz 64-81 Harvey rat sarcoma virus oncogene Mus musculus 238-244 1903226-1 1991 A study of tumors induced in mice by transplacental exposure to 7,12-dimethylbenz(a)anthracene (DMBA) alone or in combination with postnatal tissue-specific promotion showed skin and liver tumor development to be associated with cellular Ha-ras oncogene activation in a large percentage of cases. anthracene 84-94 Harvey rat sarcoma virus oncogene Mus musculus 238-244 1903226-1 1991 A study of tumors induced in mice by transplacental exposure to 7,12-dimethylbenz(a)anthracene (DMBA) alone or in combination with postnatal tissue-specific promotion showed skin and liver tumor development to be associated with cellular Ha-ras oncogene activation in a large percentage of cases. 9,10-Dimethyl-1,2-benzanthracene 96-100 Harvey rat sarcoma virus oncogene Mus musculus 238-244 2177340-0 1990 Decreased expressions of c-myc and H-ras oncogenes in vitamin E succinate induced morphologically differentiated murine B-16 melanoma cells in culture. alpha-Tocopherol 54-73 Harvey rat sarcoma virus oncogene Mus musculus 35-40 2177340-2 1990 Vitamin E succinate treatment decreased the levels of c-myc and H-ras specific mRNAs in melanoma cells. alpha-Tocopherol 0-19 Harvey rat sarcoma virus oncogene Mus musculus 64-69 2177340-5 1990 These results indicate that vitamin E succinate induced reduction of the levels of c-myc and H-ras mRNAs is related to growth inhibition of melanoma cells in culture. alpha-Tocopherol 28-47 Harvey rat sarcoma virus oncogene Mus musculus 93-98 2119910-6 1990 In total, 6 out of 35 liver tumours of male CF1 mice, two of them occurring after treatment with the tumour promoter phenobarbital solely, contained mutations at either the first or second base of codon 61 of the Ha-ras gene. Phenobarbital 117-130 Harvey rat sarcoma virus oncogene Mus musculus 213-219 2118993-1 1990 We used a dominant inhibitory mutation of c-Ha-ras which changes Ser-17 to Asn-17 in the gene product p21 [p21(Asn-17)Ha-ras] to investigate ras function in mitogenic signal transduction. Asparagine 75-78 Harvey rat sarcoma virus oncogene Mus musculus 44-50 2118993-2 1990 An NIH 3T3 cell line [NIH(M17)] was isolated that displayed inducible expression of the mutant Ha-ras gene (Ha-ras Asn-17) via the mouse mammary tumor virus long terminal repeat and was growth inhibited by dexamethasone. Asparagine 115-118 Harvey rat sarcoma virus oncogene Mus musculus 95-101 2118993-2 1990 An NIH 3T3 cell line [NIH(M17)] was isolated that displayed inducible expression of the mutant Ha-ras gene (Ha-ras Asn-17) via the mouse mammary tumor virus long terminal repeat and was growth inhibited by dexamethasone. Asparagine 115-118 Harvey rat sarcoma virus oncogene Mus musculus 108-114 2118993-2 1990 An NIH 3T3 cell line [NIH(M17)] was isolated that displayed inducible expression of the mutant Ha-ras gene (Ha-ras Asn-17) via the mouse mammary tumor virus long terminal repeat and was growth inhibited by dexamethasone. Dexamethasone 206-219 Harvey rat sarcoma virus oncogene Mus musculus 95-101 2118993-8 1990 In contrast to its effect on DNA synthesis, however, Ha-ras Asn-17 expression did not inhibit fos-cat expression induced by TPA. Asparagine 60-63 Harvey rat sarcoma virus oncogene Mus musculus 53-59 2118994-1 1990 A dominant inhibitory mutation of Ha-ras which changes Ser-17 to Asn-17 in the gene product p21 [p21 (Asn-17)Ha-ras] has been used to investigate the role of ras in neuronal differentiation of PC12 cells. Asparagine 65-68 Harvey rat sarcoma virus oncogene Mus musculus 34-40 1668891-5 1991 Oligonucleotide hybridization studies revealed activating point mutations of the c-H-ras oncogene in 40% (10/25) of tumors obtained at around 6 months. Oligonucleotides 0-15 Harvey rat sarcoma virus oncogene Mus musculus 81-88 1702367-4 1990 However, exposure of the infected cells to 3-MC resulted in a rapid cell transformation with concomitant enhancement of c-Ha-ras and H-2K class I MHC gene expression in the transformed cells. Methylcholanthrene 43-47 Harvey rat sarcoma virus oncogene Mus musculus 120-128 2219132-0 1990 Increased expression of c-myc and c-Ha-ras in dichloroacetate and trichloroacetate-induced liver tumors in B6C3F1 mice. Trichloroacetic Acid 66-82 Harvey rat sarcoma virus oncogene Mus musculus 34-42 2118247-5 1990 Transforming H-ras genes were detected in two of 6 preneoplastic HOGs and 10 of 12 carcinomas from DMBA-treated mice. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 99-103 Harvey rat sarcoma virus oncogene Mus musculus 13-18 2118892-2 1990 We obtained evidence that NIH3T3 cells transformed by the c-raf or H-ras oncogene maintained a decreased level of phosphorylation of the 80K protein, with or without phorbol ester (TPA)-stimulation, at all concentrations of serum tested while normal NIH3T3 cells maintained an elevated level of phosphorylation of the 80K protein. Phorbol Esters 166-179 Harvey rat sarcoma virus oncogene Mus musculus 67-72 2118247-11 1990 The H-ras oncogene in one DMBA-treated HOG sample was activated by A to T while the second contained an A to G mutations, representative of both modes of mutational activation involved in this model of mammary tumorigenesis. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 26-30 Harvey rat sarcoma virus oncogene Mus musculus 4-9 2198577-0 1990 Platelet-derived growth factor stimulates formation of active p21ras.GTP complex in Swiss mouse 3T3 cells. Guanosine Triphosphate 69-72 Harvey rat sarcoma virus oncogene Mus musculus 62-68 2118247-12 1990 In summary, DMBA-induced point mutated H-ras oncogenes appear to potentiate the progression of hyperplastic outgrowths (HOG) to mammary carcinomas. 6,11-dimethylbenzo(b)naphtho(2,3-d)thiophene 12-16 Harvey rat sarcoma virus oncogene Mus musculus 39-44 2191770-1 1990 The frequency and mutational profile of H-ras gene activation were determined in spontaneous liver tumors of male C57BL/6 x C3H/He mice and in tumors induced with the genotoxic hepatocarcinogen benzidine.2 HCl or the nongenotoxic hepatocarcinogens phenobarbital, chloroform, and ciprofibrate. Hydrochloric Acid 206-209 Harvey rat sarcoma virus oncogene Mus musculus 40-45 2191770-1 1990 The frequency and mutational profile of H-ras gene activation were determined in spontaneous liver tumors of male C57BL/6 x C3H/He mice and in tumors induced with the genotoxic hepatocarcinogen benzidine.2 HCl or the nongenotoxic hepatocarcinogens phenobarbital, chloroform, and ciprofibrate. Phenobarbital 248-261 Harvey rat sarcoma virus oncogene Mus musculus 40-45 2191770-1 1990 The frequency and mutational profile of H-ras gene activation were determined in spontaneous liver tumors of male C57BL/6 x C3H/He mice and in tumors induced with the genotoxic hepatocarcinogen benzidine.2 HCl or the nongenotoxic hepatocarcinogens phenobarbital, chloroform, and ciprofibrate. benzidine 194-203 Harvey rat sarcoma virus oncogene Mus musculus 40-45 2191770-1 1990 The frequency and mutational profile of H-ras gene activation were determined in spontaneous liver tumors of male C57BL/6 x C3H/He mice and in tumors induced with the genotoxic hepatocarcinogen benzidine.2 HCl or the nongenotoxic hepatocarcinogens phenobarbital, chloroform, and ciprofibrate. Chloroform 263-273 Harvey rat sarcoma virus oncogene Mus musculus 40-45 2191770-1 1990 The frequency and mutational profile of H-ras gene activation were determined in spontaneous liver tumors of male C57BL/6 x C3H/He mice and in tumors induced with the genotoxic hepatocarcinogen benzidine.2 HCl or the nongenotoxic hepatocarcinogens phenobarbital, chloroform, and ciprofibrate. ciprofibrate 279-291 Harvey rat sarcoma virus oncogene Mus musculus 40-45 2191770-2 1990 DNA sequence analysis of the H-ras gene from representative tumors revealed that 32 of 50 (64%) spontaneous tumors and 13 of 22 (59%) benzidine.2 HCl-induced tumors contained a point mutation in codon 61. benzidine 134-143 Harvey rat sarcoma virus oncogene Mus musculus 29-34 2191770-2 1990 DNA sequence analysis of the H-ras gene from representative tumors revealed that 32 of 50 (64%) spontaneous tumors and 13 of 22 (59%) benzidine.2 HCl-induced tumors contained a point mutation in codon 61. Hydrochloric Acid 146-149 Harvey rat sarcoma virus oncogene Mus musculus 29-34 2156839-0 1990 Altered phosphatidylcholine metabolism in C3H10T1/2 cells transfected with the Harvey-ras oncogene. Phosphatidylcholines 8-27 Harvey rat sarcoma virus oncogene Mus musculus 79-89 2156839-1 1990 The effect of expression of the Harvey-ras oncogene on phosphatidylcholine metabolism in C3H10T1/2 mouse fibroblast cells was examined. Phosphatidylcholines 55-74 Harvey rat sarcoma virus oncogene Mus musculus 32-42 1973614-3 1990 XbaI restriction fragment-length polymorphism analysis has shown that exposure to DMBA in utero may result in appearance of A----T transversion at the second position of codon 61 of Ha-ras oncogene in skin and liver tumors but not in lung tumors. 9,10-Dimethyl-1,2-benzanthracene 82-86 Harvey rat sarcoma virus oncogene Mus musculus 182-188 2119594-4 1990 Employing this assay, we found time- and dose-dependent induction by DMBA of Ha-ras A182----T mutation in BALB/c 3T3 cells; for example, 2 wk after exposure to 100 micrograms/mL DMBA, 1.4 in 1 X 10(4) cells contained this specific mutation. 9,10-Dimethyl-1,2-benzanthracene 69-73 Harvey rat sarcoma virus oncogene Mus musculus 77-83 2119594-6 1990 These results suggest that DMBA efficiently induces Ha-ras mutation in BALB/c 3T3 cells but that this mutation is not recruited in the process of cell transformation. 9,10-Dimethyl-1,2-benzanthracene 27-31 Harvey rat sarcoma virus oncogene Mus musculus 52-58 2191273-1 1990 The sensitivity of voltage-dependent sodium current to the sodium channel blocker tetrodotoxin (TTX) is altered by transfection of a c-Ha-ras oncogene into an excitable cell line. Sodium 37-43 Harvey rat sarcoma virus oncogene Mus musculus 133-141 2191273-1 1990 The sensitivity of voltage-dependent sodium current to the sodium channel blocker tetrodotoxin (TTX) is altered by transfection of a c-Ha-ras oncogene into an excitable cell line. Tetrodotoxin 82-94 Harvey rat sarcoma virus oncogene Mus musculus 133-141 2191273-1 1990 The sensitivity of voltage-dependent sodium current to the sodium channel blocker tetrodotoxin (TTX) is altered by transfection of a c-Ha-ras oncogene into an excitable cell line. Tetrodotoxin 96-99 Harvey rat sarcoma virus oncogene Mus musculus 133-141 2191273-3 1990 AtT-20 cells transfected with the c-Ha-ras gene expressed only a TTX-sensitive current. Tetrodotoxin 65-68 Harvey rat sarcoma virus oncogene Mus musculus 34-42 1973614-8 1990 These results indicate first that DMBA treatment may induce A----T mutation at the second position of codon 61 both in Ha-ras and in Ki-ras and, second, that the role of different activated oncogenes in carcinogenesis may differ, depending on the tissue in which the tumor develops. 9,10-Dimethyl-1,2-benzanthracene 34-38 Harvey rat sarcoma virus oncogene Mus musculus 119-125 35359864-7 2022 Among them, PIK3R1, SRC, MAPK1, AKT1, and HRAS were selected as the top 5 closely related targets to celastrol. celastrol 101-110 Harvey rat sarcoma virus oncogene Mus musculus 42-46 2204019-1 1990 The relationship of antisense oligodeoxynucleotide inhibition to the predicted secondary structure of human c-Ha-ras oncogene mRNA was examined. Oligodeoxyribonucleotides 30-50 Harvey rat sarcoma virus oncogene Mus musculus 108-116 34204734-6 2021 Conversely, knockdown of CUX1, CUX2, or SATB1 was found to be synthetic lethal in cancer cells exhibiting high ROS levels as a consequence of activating mutations in KRAS, HRAS, BRAF, or EGFR. Reactive Oxygen Species 111-114 Harvey rat sarcoma virus oncogene Mus musculus 172-176 34077012-10 2021 Furthermore, FTI-277 and GGTI-287 induced cell death in v-H-Ras-transfected NIH3T3 (NW7) cells and not in empty vector-transfected NIH3T3 (NV20) cells. FTI 277 13-20 Harvey rat sarcoma virus oncogene Mus musculus 58-63 34077012-10 2021 Furthermore, FTI-277 and GGTI-287 induced cell death in v-H-Ras-transfected NIH3T3 (NW7) cells and not in empty vector-transfected NIH3T3 (NV20) cells. GGTI 287 25-33 Harvey rat sarcoma virus oncogene Mus musculus 58-63 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Threonine 47-50 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Isoleucine 51-54 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Glutamic Acid 55-58 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Aspartic Acid 59-62 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Serine 63-66 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Tyrosine 67-70 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Arginine 71-74 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Lysine 75-78 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Glutamine 79-82 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-1 1986 A decapeptide corresponding to residues 35-44(-Thr-Ile-Glu-Asp-Ser-Tyr-Arg-Lys-Gln-Val-) of p21ras was synthesized. Valine 83-86 Harvey rat sarcoma virus oncogene Mus musculus 92-98 3096340-5 1986 The order of charged amino acid residues in the fragment 35-44 of p21ras is "complementary" to that of the substrate sequence of tyrosine-specific protein kinases (-Arg-X-X-Glu-Asp-X-X-Tyr-). Tyrosine 185-188 Harvey rat sarcoma virus oncogene Mus musculus 66-72 34912374-2 2021 In parallel, a single oral dose of DMBA (50 mg/kg bodyweight) to the same murine strain induced mammary adenocarcinomas, characterized by biphasic structures differentiated into luminal and myoepithelial lineages and frequent Hras mutations at codon 61. 9,10-Dimethyl-1,2-benzanthracene 35-39 Harvey rat sarcoma virus oncogene Mus musculus 226-230 35203289-8 2022 We identified Gnai1-3, Adyc6, Irs1, Igfr1, Hras, and Elk3 as new glucose-dependent genes. Glucose 65-72 Harvey rat sarcoma virus oncogene Mus musculus 43-47 35335874-0 2022 Chemoprevention of Urothelial Cell Carcinoma Tumorigenesis by Dietary Flavokawain A in UPII-Mutant Ha-ras Transgenic Mice. flavokawain A 70-83 Harvey rat sarcoma virus oncogene Mus musculus 99-105 35335874-3 2022 Our aim was to determine whether flavokawain A (FKA), a kava chalcone isolated from the kava plant, can target the in vivo activated Ha-ras pathway for prevention and treatment of NMIBC. flavokawain A 33-46 Harvey rat sarcoma virus oncogene Mus musculus 133-139 35335874-3 2022 Our aim was to determine whether flavokawain A (FKA), a kava chalcone isolated from the kava plant, can target the in vivo activated Ha-ras pathway for prevention and treatment of NMIBC. flavokawain A 48-51 Harvey rat sarcoma virus oncogene Mus musculus 133-139 35335874-11 2022 Overall, the results suggest that FKA can target the in vivo activated Ha-ras pathway for the prevention and treatment of NMIBC. flavokawain A 34-37 Harvey rat sarcoma virus oncogene Mus musculus 71-77 35069209-1 2021 HRas-GTP has a transient intermediate state with a "non-signaling open conformation" in GTP hydrolysis and nucleotide exchange. Guanosine Triphosphate 5-8 Harvey rat sarcoma virus oncogene Mus musculus 0-4 35069209-1 2021 HRas-GTP has a transient intermediate state with a "non-signaling open conformation" in GTP hydrolysis and nucleotide exchange. Guanosine Triphosphate 88-91 Harvey rat sarcoma virus oncogene Mus musculus 0-4