PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 1445672-2 1992 Conversion of ap-9-pivaloylfluorene, ap-(I), into lithiated (I)-9-anion followed by the addition of MeOH, then H2O, led to unexpected hydroxylation to provide 20-40% of sp-9-hydroxy-9-pivaloyfluorene, sp-(II), and 60-80% recovery of ap-(I). ap-9-pivaloylfluorene 14-35 integrin binding sialoprotein Homo sapiens 201-207 1451011-5 1992 These acidic proteins can affect the surface properties of collagen fibril, and BSP, having the cell-attachment sequence Arg-Gly-Asp, possibly mediates interaction between collagen fibril and cells. Arginine 121-124 integrin binding sialoprotein Homo sapiens 80-83 1451011-5 1992 These acidic proteins can affect the surface properties of collagen fibril, and BSP, having the cell-attachment sequence Arg-Gly-Asp, possibly mediates interaction between collagen fibril and cells. Glycine 125-128 integrin binding sialoprotein Homo sapiens 80-83 1451011-5 1992 These acidic proteins can affect the surface properties of collagen fibril, and BSP, having the cell-attachment sequence Arg-Gly-Asp, possibly mediates interaction between collagen fibril and cells. Aspartic Acid 129-132 integrin binding sialoprotein Homo sapiens 80-83 1445672-2 1992 Conversion of ap-9-pivaloylfluorene, ap-(I), into lithiated (I)-9-anion followed by the addition of MeOH, then H2O, led to unexpected hydroxylation to provide 20-40% of sp-9-hydroxy-9-pivaloyfluorene, sp-(II), and 60-80% recovery of ap-(I). (i)-9-anion 60-71 integrin binding sialoprotein Homo sapiens 201-207 1445672-2 1992 Conversion of ap-9-pivaloylfluorene, ap-(I), into lithiated (I)-9-anion followed by the addition of MeOH, then H2O, led to unexpected hydroxylation to provide 20-40% of sp-9-hydroxy-9-pivaloyfluorene, sp-(II), and 60-80% recovery of ap-(I). Methanol 100-104 integrin binding sialoprotein Homo sapiens 201-207 1445672-2 1992 Conversion of ap-9-pivaloylfluorene, ap-(I), into lithiated (I)-9-anion followed by the addition of MeOH, then H2O, led to unexpected hydroxylation to provide 20-40% of sp-9-hydroxy-9-pivaloyfluorene, sp-(II), and 60-80% recovery of ap-(I). Water 111-114 integrin binding sialoprotein Homo sapiens 201-207 1377911-2 1992 This MAb reacts with the C-terminal portion of the vWF subunit, SPII fragment [amino acids (aa) 1366-2050], which includes an Arg-Gly-Asp (RGD) sequence at positions 1744-1746, and totally inhibits vWF and SPII binding to platelet membrane glycoprotein IIb/IIIa (GPIIb/IIIa). arginyl-glycyl-aspartic acid 126-137 integrin binding sialoprotein Homo sapiens 64-68 1649377-9 1991 Some sulfated BSP was also observed in guanidine extracts and small amounts appeared to bind to collagen. Guanidine 39-48 integrin binding sialoprotein Homo sapiens 14-17 2214267-1 1990 We observed previously in humans that loading with BSP in obstructive jaundice resulted in elevated blood levels, of cysteine-conjugated BSP (Cyst-BSP). Cysteine 117-125 integrin binding sialoprotein Homo sapiens 51-54 2214267-1 1990 We observed previously in humans that loading with BSP in obstructive jaundice resulted in elevated blood levels, of cysteine-conjugated BSP (Cyst-BSP). Cysteine 117-125 integrin binding sialoprotein Homo sapiens 137-140 2214267-1 1990 We observed previously in humans that loading with BSP in obstructive jaundice resulted in elevated blood levels, of cysteine-conjugated BSP (Cyst-BSP). Cysteine 117-125 integrin binding sialoprotein Homo sapiens 137-140 34474901-6 2021 Additionally, osteoblasts responding to metabolites of CoCr-challenged endothelial cells show dynamic expression of marker genes in osteogenic differentiation, with alkaline phosphatase (ALP), osteocalcin, and bone sialoprotein (BSP) genes being significantly increased. cocr 55-59 integrin binding sialoprotein Homo sapiens 210-227 33234056-6 2021 Results Pre-treatment of BMSCs with BMP2 and BMP2/GSK126 co-stimulated expression of osteoblast-related genes (e.g., IBSP, SP7, RUNX2 and DLX5), as well as protein accumulation (e.g., collagen Type 1/COL1A1 and osteocalcin/BGLAP) based on immunofluorescence staining. GSK-2816126 50-56 integrin binding sialoprotein Homo sapiens 117-121 34810234-6 2021 IBSP microbiomes were enriched in Firmicutes and genes for amino acid and carbohydrate metabolism, but depleted in Bacteroidetes species. Carbohydrates 74-86 integrin binding sialoprotein Homo sapiens 0-4 34861472-10 2022 Mechanically, REV-ERBs activation attenuated the expression of OSX and its downstream targets including ALP, BSP and OCN. rev-erbs 14-22 integrin binding sialoprotein Homo sapiens 109-112 34474901-6 2021 Additionally, osteoblasts responding to metabolites of CoCr-challenged endothelial cells show dynamic expression of marker genes in osteogenic differentiation, with alkaline phosphatase (ALP), osteocalcin, and bone sialoprotein (BSP) genes being significantly increased. cocr 55-59 integrin binding sialoprotein Homo sapiens 229-232 35568156-10 2022 In vitro, iloprost-treated hPDLCs had a significantly increased RUNX2, OSX, BSP, and ALP gene expression that coincided with an increased deposition of mineralized nodules. Iloprost 10-18 integrin binding sialoprotein Homo sapiens 76-79 34465793-8 2021 Furthermore, our screening of a natural compound library identifies chlorogenic acid as a potent inhibitor for IBSP-receptor binding to suppress bone metastasis of ER+ tumor, suggesting its preventive use for bone recurrence in ER+ patients. Chlorogenic Acid 68-84 integrin binding sialoprotein Homo sapiens 111-115 34073955-6 2021 Similarly, protease incubation led to acute upregulation of anti-BSP immunofluorescence signals, which was blocked by cycloheximide, suggesting de novo formation of BSP. Cycloheximide 118-131 integrin binding sialoprotein Homo sapiens 65-68 34073955-6 2021 Similarly, protease incubation led to acute upregulation of anti-BSP immunofluorescence signals, which was blocked by cycloheximide, suggesting de novo formation of BSP. Cycloheximide 118-131 integrin binding sialoprotein Homo sapiens 165-168 34273166-7 2021 In vitro, nicotine induced human primary VSMC calcification, increased osteogenic gene expression (Runx2, Osx, BSP and OPN), and extracellular vesicle (EV) secretion. Nicotine 10-18 integrin binding sialoprotein Homo sapiens 111-114 35056766-10 2022 CONCLUSION: Combination treatment of TA and PAM at 75:25 ameliorated the highest enhancement of osteoblast proliferation and mineralization as well as caused a high expression of BSP and Osx genes. Tannins 37-39 integrin binding sialoprotein Homo sapiens 179-182 35222712-7 2022 Osteoblasts cultured in HG and treated with either DHA concentration displayed an improved distribution of the Col1 scaffold, increased OCN and BSP-II expression, increased NRF2 mRNA, decreased ALP activity, carbonate substitution and reduced ROS production compared with osteoblasts cultured in HG alone. Docosahexaenoic Acids 51-54 integrin binding sialoprotein Homo sapiens 144-150 35183097-0 2022 Dynamic molecular conformational change leading to energy transfer in F8-5% BSP copolymer revealed by single-molecule spectroscopy. copolymer 80-89 integrin binding sialoprotein Homo sapiens 76-79 35056766-10 2022 CONCLUSION: Combination treatment of TA and PAM at 75:25 ameliorated the highest enhancement of osteoblast proliferation and mineralization as well as caused a high expression of BSP and Osx genes. Pamidronate 44-47 integrin binding sialoprotein Homo sapiens 179-182 905194-0 1977 [Bromsulphophthalein (BSP) retention test in liver diseases]. bromsulphophthalein 1-20 integrin binding sialoprotein Homo sapiens 22-25 7457612-8 1981 BSP did not alter bile salt secretion but caused a dose-related decrease in phospholipid and cholesterol secretion. Phospholipids 76-88 integrin binding sialoprotein Homo sapiens 0-3 7457612-8 1981 BSP did not alter bile salt secretion but caused a dose-related decrease in phospholipid and cholesterol secretion. Cholesterol 93-104 integrin binding sialoprotein Homo sapiens 0-3 7457612-10 1981 Thus, BSP is hydrocholeretic but decreases phospholipid, cholesterol, and bilirubin secretion in both humans and dogs. Phospholipids 43-55 integrin binding sialoprotein Homo sapiens 6-9 7457612-10 1981 Thus, BSP is hydrocholeretic but decreases phospholipid, cholesterol, and bilirubin secretion in both humans and dogs. Cholesterol 57-68 integrin binding sialoprotein Homo sapiens 6-9 7457612-10 1981 Thus, BSP is hydrocholeretic but decreases phospholipid, cholesterol, and bilirubin secretion in both humans and dogs. Bilirubin 74-83 integrin binding sialoprotein Homo sapiens 6-9 7471656-0 1980 [Kinetics of BSP disappearance after a single dose of cimetidine in ulcer patients with liver disease]. Cimetidine 54-64 integrin binding sialoprotein Homo sapiens 13-16 7387397-1 1980 The effect of pentachlorophenol (PCP) and 2,4,6-tricholorphenol (2,4,6-T) on the disposition of the hepatodiagnostic dye, sulfobromophthalein (BSP) has been studied in isolated liver cells. 2,4,6-tricholorphenol 42-63 integrin binding sialoprotein Homo sapiens 143-146 7387397-2 1980 PCP (4-6 microM) as well as 2,4,6-T (50-100 microM) interferes with the disposition of BSP. Pentachlorophenol 0-3 integrin binding sialoprotein Homo sapiens 87-90 7387397-2 1980 PCP (4-6 microM) as well as 2,4,6-T (50-100 microM) interferes with the disposition of BSP. 2,4,6-t 28-35 integrin binding sialoprotein Homo sapiens 87-90 7387397-3 1980 The main effect apparently occurs at the secretion step as both drugs severely impair the release of the glutathione conjugate of BSP into the medium. Glutathione 105-116 integrin binding sialoprotein Homo sapiens 130-133 7387397-6 1980 Concentrations of the two phenols which interfere with the secretion of BSP also completely uncouple the oxidative phosphorylation of hepatocellular mitochondria. Phenols 26-33 integrin binding sialoprotein Homo sapiens 72-75 2712362-8 1989 Immunoprecipitates of SDS-electrophoretically separated and radiolabeled sperm surface antigens revealed a molecular weight of 18 KD for Ki-Sp II-13 and of 24 KD for Ki-Sp VI-2. Sodium Dodecyl Sulfate 22-25 integrin binding sialoprotein Homo sapiens 140-145 33925774-9 2021 RT-PCR analysis revealed that on day 8, both osteogenic (ALP, RUNX-2, and BSP) and angiogenic genes (VEGFR-2, CD31) increased significantly in the IPE group as compared to the IP or IE groups (p < 0.05). ipe 147-150 integrin binding sialoprotein Homo sapiens 74-77 5160356-0 1971 [Comparative liver function studies with high doses of BSP in intravenous galactose loading]. Galactose 74-83 integrin binding sialoprotein Homo sapiens 55-58 5203499-0 1970 [Successive abdominal scintillation with 131-I-tagged BSP--description of the thyroid gland, kidney and spleen (comparison with 131-I-RB)]. Iodine 45-46 integrin binding sialoprotein Homo sapiens 54-57 5786021-0 1969 [Binding capacity of serum albumin for BSP in patients with liver disease measured with gel filtration on sephadex G 25]. sephadex 106-114 integrin binding sialoprotein Homo sapiens 39-42 13649197-0 1959 [Respective value of the elimination rate of BSP & biligraffin in the diagnosis of diseases of bile ducts]. biligraffin 55-66 integrin binding sialoprotein Homo sapiens 45-48 33968185-8 2021 The effects of Pg-LPS on the mRNA expression of the calcification genes of ALP, core-binding factor alpha1 (Runx2) and bone sialoprotein (BSP) were assessed using reverse transcription-quantitative PCR. pg-lps 15-21 integrin binding sialoprotein Homo sapiens 119-136 33968185-8 2021 The effects of Pg-LPS on the mRNA expression of the calcification genes of ALP, core-binding factor alpha1 (Runx2) and bone sialoprotein (BSP) were assessed using reverse transcription-quantitative PCR. pg-lps 15-21 integrin binding sialoprotein Homo sapiens 138-141 33968185-11 2021 In addition, the expression of specific osteogenic genes (Runx2, ALP and BSP) significantly increased in the presence of Pg-LPS and mineralization-inducing medium, which was further enhanced in co-culture with HPDLCs. pg-lps 121-127 integrin binding sialoprotein Homo sapiens 73-76 604160-0 1977 The effect of BSP and rifamycin on the excretion of warfarin in the bile of the rate. Warfarin 52-60 integrin binding sialoprotein Homo sapiens 14-17 4772552-0 1973 [Effect of glucose refeeding after fasting on bilirubin and BSP]. Glucose 11-18 integrin binding sialoprotein Homo sapiens 60-63 5547757-2 1971 Saramycetin, a polypeptide antifungal antibiotic has been found to retard the clearance of sulphobromophthalein (BSP) in man. saramycetin 0-11 integrin binding sialoprotein Homo sapiens 113-116 5547757-2 1971 Saramycetin, a polypeptide antifungal antibiotic has been found to retard the clearance of sulphobromophthalein (BSP) in man. Sulfobromophthalein 91-111 integrin binding sialoprotein Homo sapiens 113-116 5547757-5 1971 Saramycetin strongly inhibited the hepatic enzyme which conjugates BSP to reduced glutathione, provoked a regurgitation of BSP from the liver into the bloodstream, and was anticholeretic in the dog.4. saramycetin 0-11 integrin binding sialoprotein Homo sapiens 67-70 5547757-5 1971 Saramycetin strongly inhibited the hepatic enzyme which conjugates BSP to reduced glutathione, provoked a regurgitation of BSP from the liver into the bloodstream, and was anticholeretic in the dog.4. saramycetin 0-11 integrin binding sialoprotein Homo sapiens 123-126 5547757-5 1971 Saramycetin strongly inhibited the hepatic enzyme which conjugates BSP to reduced glutathione, provoked a regurgitation of BSP from the liver into the bloodstream, and was anticholeretic in the dog.4. Glutathione 82-93 integrin binding sialoprotein Homo sapiens 67-70 5547757-6 1971 These diverse actions of Saramycetin may, in concert, explain the altered clearance of BSP. saramycetin 25-36 integrin binding sialoprotein Homo sapiens 87-90 5191734-2 1969 Dye excretion studies using sulfobromophthalein (BSP). Sulfobromophthalein 28-47 integrin binding sialoprotein Homo sapiens 49-52 33743412-8 2021 RESULTS: ICA significantly promoted hBMSC osteogenic differentiation by upregulating alkaline phosphatase activity and the expression of bone sialoprotein II (BSPII) and runt-related transcription factor-2 (Runx-2). icariin 9-12 integrin binding sialoprotein Homo sapiens 137-157 33743412-8 2021 RESULTS: ICA significantly promoted hBMSC osteogenic differentiation by upregulating alkaline phosphatase activity and the expression of bone sialoprotein II (BSPII) and runt-related transcription factor-2 (Runx-2). icariin 9-12 integrin binding sialoprotein Homo sapiens 159-164 32163873-3 2020 Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. Hypochlorous Acid 86-90 integrin binding sialoprotein Homo sapiens 38-41 33610546-0 2021 A repeated triple lysine motif anchors complexes containing bone sialoprotein and the type XI collagen A1 chain involved in bone mineralization. Lysine 18-24 integrin binding sialoprotein Homo sapiens 60-77 33610546-3 2021 To learn more, we examined the protein composition of extracellular sites of de novo hydroxyapatite deposition which were enriched in BSP and Col11a1 containing an alternatively spliced "6b" exonal sequence. Durapatite 85-99 integrin binding sialoprotein Homo sapiens 134-137 33610546-9 2021 Mass spectroscopic mapping demonstrated that FAM-peptide 3 bound to 90 kDa BSP and its 35-60 kDa fragments, as well as to 110 kDa nucleolin. fam-peptide 45-56 integrin binding sialoprotein Homo sapiens 75-78 32629962-6 2020 Surprisingly, the upregulation of cAMP level at the early stages of osteogenic differentiation downregulated the expression of osteogenic markers RUNX2, Osterix, and IBSP, which was more significant in spheroids, and it is used for the more efficient commitment of ADSCs into preosteoblasts, according to the previously reported protocol. Cyclic AMP 34-38 integrin binding sialoprotein Homo sapiens 166-170 33159588-8 2020 In the expressions of the Bsp and Bglap2 genes, for the group containing ALN (TiO2 + ALN), it was observed an increase in expression (approximately sixfold change) when compared to the control. titanium dioxide 78-82 integrin binding sialoprotein Homo sapiens 26-29 33159588-8 2020 In the expressions of the Bsp and Bglap2 genes, for the group containing ALN (TiO2 + ALN), it was observed an increase in expression (approximately sixfold change) when compared to the control. Alendronate 73-76 integrin binding sialoprotein Homo sapiens 26-29 32715131-8 2020 Alendronate-modified surfaces promoted a significant increase on ECM remodeling, as well as culminating with greater gene activity related to the osteogenic phenotype (Runx2, Alp, Bsp). Alendronate 0-11 integrin binding sialoprotein Homo sapiens 180-183 32163873-3 2020 Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. Hypochlorous Acid 103-107 integrin binding sialoprotein Homo sapiens 38-41 32163873-3 2020 Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. methylphenylsulfide 129-150 integrin binding sialoprotein Homo sapiens 38-41 32163873-3 2020 Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCl through the HOCl-induced oxidation of methyl phenyl sulfide to sulfoxide. sulfoxide 154-163 integrin binding sialoprotein Homo sapiens 38-41 32163873-4 2020 The reaction of BSP with HOCl caused a 22-fold fluorescence enhancement (quantum yield increase from 0.006 to 0.133). Hypochlorous Acid 25-29 integrin binding sialoprotein Homo sapiens 16-19 32163873-7 2020 Eventually, the cellular fluorescence imaging experiment showed that BSP could be effectively used for monitoring HOCl in living cells. Hypochlorous Acid 114-118 integrin binding sialoprotein Homo sapiens 69-72 32498305-6 2020 We report here the potential of fullerene (C60)-polyethyleneimine (PEI)/short hairpin (sh)RNA-Runx2 nano-polyplexes to efficiently down-regulate Runx2 mRNA and protein expression leading subsequently to a significant reduction in the expression of osteogenic proteins (i.e. ALP, BSP, OSP and BMP4) in osteoblast-committed VIC. Fullerenes 32-41 integrin binding sialoprotein Homo sapiens 279-282 32498305-6 2020 We report here the potential of fullerene (C60)-polyethyleneimine (PEI)/short hairpin (sh)RNA-Runx2 nano-polyplexes to efficiently down-regulate Runx2 mRNA and protein expression leading subsequently to a significant reduction in the expression of osteogenic proteins (i.e. ALP, BSP, OSP and BMP4) in osteoblast-committed VIC. -polyethyleneimine 47-65 integrin binding sialoprotein Homo sapiens 279-282 32498305-6 2020 We report here the potential of fullerene (C60)-polyethyleneimine (PEI)/short hairpin (sh)RNA-Runx2 nano-polyplexes to efficiently down-regulate Runx2 mRNA and protein expression leading subsequently to a significant reduction in the expression of osteogenic proteins (i.e. ALP, BSP, OSP and BMP4) in osteoblast-committed VIC. pei 67-70 integrin binding sialoprotein Homo sapiens 279-282 31158404-5 2019 Oral administration with 100 mg/kg of BSP for 3 days continuously could significantly prevent the formation of ethanol-induced gastric mucosal lesion. Ethanol 111-118 integrin binding sialoprotein Homo sapiens 38-41 32555274-11 2020 Expression levels of BMP-2 and BSP were significantly upregulated by OIM and Si (25 mug/ml) and were also induced by Si alone. Silicon 77-79 integrin binding sialoprotein Homo sapiens 31-34 31939626-9 2020 The mRNA expression of COL1A1, BSP and BMP2 exhibited significant upregulation from day 7 post-KR-12-a6 treatment. kr-12-a6 95-103 integrin binding sialoprotein Homo sapiens 31-34 31269454-7 2019 However, the macrophage-CDHA interaction resulted in a more favourable environment for osteogenic differentiation of osteoblasts with more collagen type I production and osteogenic genes (Runx2, BSP) expression, suggesting that osteogenic differentiation of bone cells is not only determined by the nature of biomaterials, but also significantly influenced by the inflammatory environment generated by the interaction of immune cells and biomaterials. cdha 24-28 integrin binding sialoprotein Homo sapiens 195-198 31158404-1 2019 The chemical characterization and protective role against ethanol-induced gastric ulcerated rats of a polysaccharide fraction from Bletilla striata (BSP) collected by ultrafiltration membrane approach were evaluated. Polysaccharides 102-116 integrin binding sialoprotein Homo sapiens 149-152 31158404-7 2019 Additionally, the BSP faction exhibited antioxidant activity, increased the content of PEG2 as a defensive factor, and suppressed MAPK/NF-kappaB signaling pathway in gastric tissue. peg2 87-91 integrin binding sialoprotein Homo sapiens 18-21 31158404-2 2019 This BSP faction was consisted of mannose and glucose at a molar ratio of 2.4:1 approximately, with a molecular weight of 146 KDa. Mannose 34-41 integrin binding sialoprotein Homo sapiens 5-8 31158404-2 2019 This BSP faction was consisted of mannose and glucose at a molar ratio of 2.4:1 approximately, with a molecular weight of 146 KDa. Glucose 46-53 integrin binding sialoprotein Homo sapiens 5-8 31293042-11 2019 Furthermore, 50 muM metformin significantly upregulated the gene expression levels of ALP, BSP, OPN, OCN, and Runx2 and the protein expression of ALP and Runx2 (P < 0.05). Metformin 20-29 integrin binding sialoprotein Homo sapiens 91-94 31158404-3 2019 FT-IR, NMR and XRD spectra indicated that BSP faction contained alpha-Man and beta-Glc residues with low overall crystallinity. beta-glc 78-86 integrin binding sialoprotein Homo sapiens 42-45 31114166-8 2019 On day 7, 5 muM EGCG significantly enhanced BSP expression. epigallocatechin gallate 16-20 integrin binding sialoprotein Homo sapiens 44-47 30988819-8 2019 Following treatment of both cell lines with SB225002, western blotting and RT-qPCR results indicated that the expression levels of BSP protein and mRNA were significantly downregulated. SB 225002 44-52 integrin binding sialoprotein Homo sapiens 131-134 31105837-7 2019 However, vitamin C stimulated lower Cbfa1, Col-1, ALP, OPN, BSP, OCN, VDR, CEMP-1 and IL-6 mRNA fold-changes than 10-8 M calcitriol. Ascorbic Acid 9-18 integrin binding sialoprotein Homo sapiens 60-63 28945007-4 2017 While DMH1 inhibits runt-related transcription factor 2 (Runx2) and bone sialoprotein II (BSPII) gene expression of primary human mesenchymal stem cells (hMSCs) on MC-GAG, PD98059 inhibits BSPII expression on Col-GAG independent of Runx2 expression. mc-gag 164-170 integrin binding sialoprotein Homo sapiens 90-95 30800672-11 2019 Additionally, L-C induced the phosphorylation of ERK1/2 and AKT and the main kinases involved in osteoblastic differentiation and upregulated the expression of osteogenic related genes, RUNX2, osterix (OSX), bone sialoprotein (BSP), and osteopontin (OPN) as well as OPN protein synthesis, suggesting that L-C exerts a positive modulation of key osteogenic factors. Carnitine 14-17 integrin binding sialoprotein Homo sapiens 208-225 30800672-11 2019 Additionally, L-C induced the phosphorylation of ERK1/2 and AKT and the main kinases involved in osteoblastic differentiation and upregulated the expression of osteogenic related genes, RUNX2, osterix (OSX), bone sialoprotein (BSP), and osteopontin (OPN) as well as OPN protein synthesis, suggesting that L-C exerts a positive modulation of key osteogenic factors. Carnitine 14-17 integrin binding sialoprotein Homo sapiens 227-230 30317648-7 2019 We found that exogenous cholesterol significantly inhibited alkaline phosphatase (ALP) activity and messenger RNA expression of osteoblast markers genes (Alpl, Sp7, and Ibsp) while modestly activating expression of Gli1 (a readout of Hh signaling) under both basal osteogenic culture condition and Wnt3a treatment. Cholesterol 24-35 integrin binding sialoprotein Homo sapiens 169-173 31352121-6 2019 Silencing of miR-187-3p dramatically accelerated hFOB 1.19 osteoblastic differentiation, as evidenced by the increase of alkaline phosphatase (ALP) activity and calcium deposition, as well as elevated osteopontin (OPN), collagen type I alpha 1 (COL1A1), and bone sialoprotein (BSP) gene expression, whereas overexpression of miR-187-3p suppressed osteoblastic differentiation. mir-187-3p 13-23 integrin binding sialoprotein Homo sapiens 258-275 31352121-6 2019 Silencing of miR-187-3p dramatically accelerated hFOB 1.19 osteoblastic differentiation, as evidenced by the increase of alkaline phosphatase (ALP) activity and calcium deposition, as well as elevated osteopontin (OPN), collagen type I alpha 1 (COL1A1), and bone sialoprotein (BSP) gene expression, whereas overexpression of miR-187-3p suppressed osteoblastic differentiation. mir-187-3p 13-23 integrin binding sialoprotein Homo sapiens 277-280 30585266-5 2018 The lower NADH level in atrophic nonunion tissues disrupted CtBP2 dimerization and enhanced the blockage of the accessibility of the p300-Runx2 complex to the promoters of a series of bone-related target genes, such as OSC, ALPL, COL1A1, IBSP, SPP1 and MMP13. NAD 10-14 integrin binding sialoprotein Homo sapiens 238-242 29316208-0 2018 Effect of bone sialoprotein coated three-dimensional printed calcium phosphate scaffolds on primary human osteoblasts. calcium phosphate 61-78 integrin binding sialoprotein Homo sapiens 10-27 29316208-4 2018 3D-plotted calcium phosphate cement (CPC) scaffolds were coated with BSP via physisorption. calcium phosphate 11-28 integrin binding sialoprotein Homo sapiens 69-72 29316208-4 2018 3D-plotted calcium phosphate cement (CPC) scaffolds were coated with BSP via physisorption. cpc 37-40 integrin binding sialoprotein Homo sapiens 69-72 29316208-11 2018 Quantitative cell morphology analyses demonstrated for BSP-coated CPCs an enhanced cell area and reduced circularity. cytidylyl-(3'-5')-cytidine 66-70 integrin binding sialoprotein Homo sapiens 55-58 29764806-2 2018 An important risk factor for breast cancer is individual genetic background, which is initially generated early in human life, for example, during the processes of embryogenesis and fetal development in utero Bioactive dietary components such as sulforaphane (SFN), an isothiocyanate from cruciferous vegetables including broccoli sprouts (BSp), cabbage, and kale, has been shown to reduce the risk of developing many common cancers through regulation of epigenetic mechanisms. sulforaphane 246-258 integrin binding sialoprotein Homo sapiens 340-343 29917166-0 2018 SB225002 inhibits prostate cancer invasion and attenuates the expression of BSP, OPN and MMP-2. SB 225002 0-8 integrin binding sialoprotein Homo sapiens 76-79 29917166-5 2018 In the present study, we investigated whether SB225002, a specific CXCR2 receptor antagonist, can inhibit prostate cancer cell expression of BSP and OPN and reduce cancer cell invasion ability. SB 225002 46-54 integrin binding sialoprotein Homo sapiens 141-144 29305863-8 2018 In addition, Fra-1 overexpression alleviates the inhibitory effect of miR-449 mimics on the ALP activity and the mRNA and protein of Runx2, collagen I, OCN and BSP. mir-449 70-77 integrin binding sialoprotein Homo sapiens 160-163 29060818-6 2017 In SP/SN group, the recency effect is found in AWI. Tin 6-8 integrin binding sialoprotein Homo sapiens 3-5 29211290-10 2017 The mRNA level of BSP has increased significantly in MTA with MC/CaCl2 compared to the control (p<.05). Methylcellulose 62-64 integrin binding sialoprotein Homo sapiens 18-21 29211290-10 2017 The mRNA level of BSP has increased significantly in MTA with MC/CaCl2 compared to the control (p<.05). Calcium Chloride 65-70 integrin binding sialoprotein Homo sapiens 18-21 29188650-10 2017 The inhibition of ERK1/2 by U0126 and DN-ERK1/2 suppressed the expressionof Runx2 mRNA, OCN mRNA, BSP mRNA, Runx 2 protein, and p-ERK1/2 protein relative to that in stretched cells without the ERK1/2 inhibitor. U 0126 28-33 integrin binding sialoprotein Homo sapiens 98-101 28958711-4 2017 Herein, we employed tandem mass spectrometry (MS/MS) to demonstrate that the N-glycanson the recombinant human integrin binding sialoprotein (rhiBSP) are also enriched in sialic acids (SAs) at their termini. n-glycanson 77-88 integrin binding sialoprotein Homo sapiens 111-140 28958711-4 2017 Herein, we employed tandem mass spectrometry (MS/MS) to demonstrate that the N-glycanson the recombinant human integrin binding sialoprotein (rhiBSP) are also enriched in sialic acids (SAs) at their termini. Sialic Acids 171-183 integrin binding sialoprotein Homo sapiens 111-140 28958711-9 2017 Collectively, our results identified novel N-glycans enriched in SAs on the rhiBSP and demonstrated that SAs at both N- and O-glycans are important for BSP regulation of osteoblast differentiation and mineralization in vitro. n-glycans 43-52 integrin binding sialoprotein Homo sapiens 79-82 27771827-9 2017 Interestingly, it was observed that rhBMP9 induced significantly higher ALP activity, alizarin red staining, and messenger RNA (mRNA) levels of ALP and BSP when compared to rhBMP2. rhbmp9 36-42 integrin binding sialoprotein Homo sapiens 152-155 27569283-13 2016 Furthermore, l-thyroxine enhanced the expression of COL1A1, Runx2, OC, BSP, Dlx5, and OSX mRNA and proteins. Thyroxine 13-24 integrin binding sialoprotein Homo sapiens 71-74 32263532-6 2016 The proliferation, alkaline phosphatase, osteogenesis-related proteins (OPN and BSP), and angiogenesis-related protein (vWF and ang-1) secretion of hMSCs were significantly stimulated when the PDA-coated concentration was increased. polydopamine 193-196 integrin binding sialoprotein Homo sapiens 80-83 26849824-7 2016 The expression and the activity of OPG, BSP, and FGF23 were also reduced by pioglitazone together with total (but not specific) calcium and phosphate content. Pioglitazone 76-88 integrin binding sialoprotein Homo sapiens 40-43 27104488-5 2016 Assuming that sulfate was present as ammonium bisulfate rather than as ammonium sulfate uniformly reduced estimated Bsp. Sulfates 14-21 integrin binding sialoprotein Homo sapiens 116-119 27104488-5 2016 Assuming that sulfate was present as ammonium bisulfate rather than as ammonium sulfate uniformly reduced estimated Bsp. ammonium bisulfate 37-55 integrin binding sialoprotein Homo sapiens 116-119 27104488-5 2016 Assuming that sulfate was present as ammonium bisulfate rather than as ammonium sulfate uniformly reduced estimated Bsp. Ammonium Sulfate 71-87 integrin binding sialoprotein Homo sapiens 116-119 28105418-5 2016 A concentration of 10-8 M calcitriol enhanced ALP, CBFA1, Col-1, and OCN mRNA expression at both weeks and BSP mRNA expression at the first week. Calcitriol 26-36 integrin binding sialoprotein Homo sapiens 107-110 32262965-10 2016 Furthermore, the beta-TCP-Fe-GO scaffolds significantly enhanced alkaline phosphatase (ALP) activity and osteogenic gene expression, such as OPN, Runx2, OCN and BSP, of rabbit bone marrow stromal cells (rBMSCs) and significantly stimulated rBMSCs proliferation as compared to pure beta-TCP scaffolds by the synergistic effect of GO and the released Fe ions. beta-tcp-fe-go 17-31 integrin binding sialoprotein Homo sapiens 161-164 32262965-10 2016 Furthermore, the beta-TCP-Fe-GO scaffolds significantly enhanced alkaline phosphatase (ALP) activity and osteogenic gene expression, such as OPN, Runx2, OCN and BSP, of rabbit bone marrow stromal cells (rBMSCs) and significantly stimulated rBMSCs proliferation as compared to pure beta-TCP scaffolds by the synergistic effect of GO and the released Fe ions. beta-tricalcium phosphate 17-25 integrin binding sialoprotein Homo sapiens 161-164 32262965-10 2016 Furthermore, the beta-TCP-Fe-GO scaffolds significantly enhanced alkaline phosphatase (ALP) activity and osteogenic gene expression, such as OPN, Runx2, OCN and BSP, of rabbit bone marrow stromal cells (rBMSCs) and significantly stimulated rBMSCs proliferation as compared to pure beta-TCP scaffolds by the synergistic effect of GO and the released Fe ions. Iron 26-28 integrin binding sialoprotein Homo sapiens 161-164 27111551-5 2016 In this study, BSP was covalently coupled to titanium surfaces via an aminosilane linker (APTES), and its properties were compared to BSP applied to titanium via physisorption and untreated titanium. 2-cyanoethyldimethyl(diethyl)aminosilane 70-81 integrin binding sialoprotein Homo sapiens 15-18 27111551-8 2016 Cell adhesion to titanium surfaces treated with BSP via physisorption was not significantly different from that of untreated titanium at any time point, whereas BSP application via covalent coupling caused reduced cell adhesion during the first few hours in culture. Titanium 17-25 integrin binding sialoprotein Homo sapiens 48-51 27111551-9 2016 Cell migration was increased on titanium disks that were treated with higher concentrations of BSP solution, independent of the coating method. Titanium 32-40 integrin binding sialoprotein Homo sapiens 95-98 27111551-10 2016 During the early phases of hOB proliferation, a suppressive effect of BSP was observed independent of its concentration, particularly when BSP was applied to the titanium surface via physisorption. Titanium 162-170 integrin binding sialoprotein Homo sapiens 70-73 27111551-10 2016 During the early phases of hOB proliferation, a suppressive effect of BSP was observed independent of its concentration, particularly when BSP was applied to the titanium surface via physisorption. Titanium 162-170 integrin binding sialoprotein Homo sapiens 139-142 27111551-11 2016 Although alkaline phosphatase activity was reduced in the BSP-coated titanium groups after 4 days in culture, increased calcium deposition was observed after 21 days. Titanium 69-77 integrin binding sialoprotein Homo sapiens 58-61 26923821-7 2016 Osteogenically committed hBMSC-EVs induced an osteogenic phenotype characterized by marked early induction of BMP2, SP7, SPP1, BGLAP/IBSP, and alkaline phosphatase. hbmsc-evs 25-34 integrin binding sialoprotein Homo sapiens 133-137 25837183-4 2015 RESULTS: The results showed that ZXHA-TC has a stimulating effect on the proliferation and osteogenic differentiation of primary osteoblasts by promoting cell proliferation, cell viability, and the expression of genes BSP and OCN. zxha-tc 33-40 integrin binding sialoprotein Homo sapiens 218-221 26147021-9 2015 RESULTS: Nanoparticulate mineralization of collagen-glycosaminoglycan scaffolds increased expression of both OCN and BSP. collagen-glycosaminoglycan 43-69 integrin binding sialoprotein Homo sapiens 117-120 32262393-5 2015 The three functional domains of (MBP)-BSP-HAP provide the artificial protein with multiple designated functions for intrafibrillar mineralization including binding calcium ions, binding collagen, and binding hydroxyapatite. Calcium 164-171 integrin binding sialoprotein Homo sapiens 38-41 32262393-6 2015 Platelet-like hydroxyapatite crystals periodically arranged inside the collagen fibrils have been achieved under the function of (MBP)-BSP-HAP. Durapatite 14-28 integrin binding sialoprotein Homo sapiens 135-138 26420877-6 2015 Parbendazole stimulates osteoblast differentiation as indicated by increased alkaline phosphatase activity, mineralization, and up-regulation of bone marker genes (alkaline phosphatase/ALPL, osteopontin/SPP1, and bone sialoprotein II/IBSP) in a subset of the hMSC population resistant to the apoptotic effects of parbendazole. parbendazole 0-12 integrin binding sialoprotein Homo sapiens 213-233 26420877-6 2015 Parbendazole stimulates osteoblast differentiation as indicated by increased alkaline phosphatase activity, mineralization, and up-regulation of bone marker genes (alkaline phosphatase/ALPL, osteopontin/SPP1, and bone sialoprotein II/IBSP) in a subset of the hMSC population resistant to the apoptotic effects of parbendazole. parbendazole 0-12 integrin binding sialoprotein Homo sapiens 234-238 25816130-7 2015 It was revealed that GTX was able to block TNF-alpha-induced inhibition of osteoblast differentiation, as indicated by alkaline phosphatase (ALP) activity and ALP staining assays, as well as the expression levels of osteoblast-associated genes Col I, Ocn, Bsp, Runx2, Osx and ATF4. Gliotoxin 21-24 integrin binding sialoprotein Homo sapiens 256-259 25264744-7 2014 Real-time reverse transcription-polymerase chain reaction showed that melatonin increased the expression of mRNAs for osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), dentin matrix protein-1 (DMP-1) and dentin sialophosphoprotin (DSPP). Melatonin 70-79 integrin binding sialoprotein Homo sapiens 156-173 26103903-3 2015 Limiting dilution (LD) bisulfite Pyrosequencing( ) (BSP) is a relatively simple technique to circumvent these problems. hydrogen sulfite 23-32 integrin binding sialoprotein Homo sapiens 52-55 25230055-1 2015 OBJECTIVES: The aim of this study was to estimate the effective absorbed radiation dose to human organs following promising in-vivo results of intravenous administration of 4-benzyl-1-(3-[125I]-iodobenzylsulfonyl)piperidine (4-B-[125I]-IBSP) using normal biodistribution data obtained from rats. 4-benzyl-1-(3-iodobenzylsulfonyl)piperidine 173-223 integrin binding sialoprotein Homo sapiens 236-240 32261896-4 2014 The incorporation of magnetic Fe3O4 nanoparticles into MBG/PCL scaffolds did not influence their apatite mineralization ability but endowed excellent magnetic heating ability and significantly stimulated proliferation, alkaline phosphatase (ALP) activity, osteogenesis-related gene expression (RUNX2, OCN, BSP, BMP-2 and Col-1) and extra-cellular matrix (ECM) mineralization of human bone marrow-derived mesenchymal stem cells (h-BMSCs). ferryl iron 30-35 integrin binding sialoprotein Homo sapiens 306-309 25264744-7 2014 Real-time reverse transcription-polymerase chain reaction showed that melatonin increased the expression of mRNAs for osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP), dentin matrix protein-1 (DMP-1) and dentin sialophosphoprotin (DSPP). Melatonin 70-79 integrin binding sialoprotein Homo sapiens 175-178 24115157-4 2014 The levels of methylation of lysines 4 and 36 and acetylation of histone H3, markers for active chromatin, were also reduced at the Bsp gene in these cells. Lysine 29-36 integrin binding sialoprotein Homo sapiens 132-135 24980816-3 2014 Conditional MDA-MB-231 subclones were equipped with a novel gene expression cassette containing a tet-reg-ulated miRNA providing knockdown of BSP production. tetramethylenedisulfotetramine 98-101 integrin binding sialoprotein Homo sapiens 142-145 24935539-7 2014 The MAPK inhibitor for extracellular signal-regulated kinase 1/2 (U0126) and Jun N-terminal kinase (SP600125) significantly decreased the Biodentine-induced mineralized differentiation of hDPSCs and OCN, DSPP, DMP1, and BSP messenger RNA expression, whereas p38 MAPK inhibitors (SB203580) had no effect. pyrazolanthrone 100-108 integrin binding sialoprotein Homo sapiens 220-223 24935539-7 2014 The MAPK inhibitor for extracellular signal-regulated kinase 1/2 (U0126) and Jun N-terminal kinase (SP600125) significantly decreased the Biodentine-induced mineralized differentiation of hDPSCs and OCN, DSPP, DMP1, and BSP messenger RNA expression, whereas p38 MAPK inhibitors (SB203580) had no effect. tricalcium silicate 138-148 integrin binding sialoprotein Homo sapiens 220-223 24591126-4 2014 Among more than 100 oxysterol analogues synthesized, Oxy133 induced significant expression of osteogenic markers Runx2, osterix (OSX), alkaline phosphatase (ALP), bone sialoprotein (BSP), and osteocalcin (OCN) in C3H10T1/2 mouse embryonic fibroblasts and in M2-10B4 mouse marrow stromal cells. Oxy133 53-59 integrin binding sialoprotein Homo sapiens 182-185 24591126-7 2014 Oxy133 induced the expression of OSX, BSP, and OCN, and stimulated robust mineralization in primary human mesenchymal stem cells. Oxy133 0-6 integrin binding sialoprotein Homo sapiens 38-41 24935539-6 2014 RESULTS: Biodentine significantly increased alkaline phosphatase activity and mineralized nodule formation and the expression of OCN, DSPP, DMP1, and BSP. tricalcium silicate 9-19 integrin binding sialoprotein Homo sapiens 150-153 23738684-10 2014 However, the expression of BSP and RUNX2 genes was higher at 7 d compared to 3 d. Significant calcium mineralization was detected as quantified by alizarin red assay at 14 d in 1 : 1 (1323.55 +- 6.54 mum) and 5 : 1 (994.67 +- 4.15 mum) co-cultures as compared with monoculture cell sheets (p < 0.05). Calcium 94-101 integrin binding sialoprotein Homo sapiens 27-30 23738684-10 2014 However, the expression of BSP and RUNX2 genes was higher at 7 d compared to 3 d. Significant calcium mineralization was detected as quantified by alizarin red assay at 14 d in 1 : 1 (1323.55 +- 6.54 mum) and 5 : 1 (994.67 +- 4.15 mum) co-cultures as compared with monoculture cell sheets (p < 0.05). alizarin 147-159 integrin binding sialoprotein Homo sapiens 27-30 24747566-6 2014 The degree of osteogenic differentiation of BM-MSCs and PL-MSCs after bortezomib treatment was assessed by alkaline phosphatase (ALP) activity, matrix mineralization by Alizarin Red S staining and the expression profiles of osteogenic differentiation marker genes, Osterix, RUNX2 and BSP. Bortezomib 70-80 integrin binding sialoprotein Homo sapiens 284-287 24747566-7 2014 The results showed that 1 nM and 2 nM BZB can induce osteogenic differentiation of BM-MSCs and PL-MSCs as demonstrated by increased ALP activity, increased matrix mineralization and up-regulation of osteogenic differentiation marker genes, Osterix, RUNX2 and BSP as compared to controls. Bortezomib 38-41 integrin binding sialoprotein Homo sapiens 259-262 24103036-6 2013 These results suggest that the N-terminal RGD-flanking region of hBSP peptide 278-293, in particular the tyrosine-278 residue, represents a second cell-attachment site that stabilizes the RGD-integrin receptor complex. Tyrosine 105-113 integrin binding sialoprotein Homo sapiens 65-69 24739710-0 2014 Melatonin regulates human bone sialoprotein gene transcription. Melatonin 0-9 integrin binding sialoprotein Homo sapiens 26-43 24739710-3 2014 To elucidate the effects of melatonin on human BSP gene expression, we utilized human Saos2 osteoblast-like cells. Melatonin 28-37 integrin binding sialoprotein Homo sapiens 47-50 24739710-4 2014 Melatonin (100 nM) increased the level of BSP mRNA at 3 h, and the level became maximal at 12 and 24 h. We then investigated the melatonin-induced transcriptional activity of luciferase constructs (between -84LUC and -868LUC) including different lengths of the human BSP gene promoter transfected into Saos2 cells. Melatonin 0-9 integrin binding sialoprotein Homo sapiens 42-45 24739710-4 2014 Melatonin (100 nM) increased the level of BSP mRNA at 3 h, and the level became maximal at 12 and 24 h. We then investigated the melatonin-induced transcriptional activity of luciferase constructs (between -84LUC and -868LUC) including different lengths of the human BSP gene promoter transfected into Saos2 cells. Melatonin 0-9 integrin binding sialoprotein Homo sapiens 267-270 24739710-8 2014 These data indicate that melatonin induces BSP transcription via the CRE1 and CRE2 elements in the human BSP gene promoter. Melatonin 25-34 integrin binding sialoprotein Homo sapiens 43-46 24739710-8 2014 These data indicate that melatonin induces BSP transcription via the CRE1 and CRE2 elements in the human BSP gene promoter. Melatonin 25-34 integrin binding sialoprotein Homo sapiens 105-108 24103036-7 2013 Computer modeling also suggested that a beta-turn encompassing RGD or RGE in some of the hBSP peptides may facilitate its binding to integrins by increasing the exposure of the tripeptide. tripeptide K-26 177-187 integrin binding sialoprotein Homo sapiens 89-93 23904377-9 2013 Pearson"s analysis showed that serum BSP level was positively correlated with VAS in PC patients with BM (P<0.05). vas 78-81 integrin binding sialoprotein Homo sapiens 37-40 32260928-5 2013 Our results showed that the ionic products from bredigite powder extracts led to significantly enhanced proliferation and cementogenic differentiation, including mineralization-nodule formation, ALP activity and a series of bone/cementum-related gene/protein expression (ALP, OPN, OCN, BSP, CAP and CEMP1) of PDLCs in a concentration dependent manner. tremolite 48-57 integrin binding sialoprotein Homo sapiens 286-289 22545813-2 2012 Here we proposed to develop a lipid prodrug based on a polyamine analogue bisethylnorspermine (BSP) that can function dually as gene delivery vector and, after intracellular degradation, as active anticancer agent targeting dysregulated polyamine metabolism. Polyamines 55-64 integrin binding sialoprotein Homo sapiens 95-98 23261880-3 2013 Mature BSP shows extensive post-translational modifications, including attachment of glycans, sulfation, and phosphorylation, and is highly flexible with no specific 2D or 3D structure in solution or the solid state. Polysaccharides 85-92 integrin binding sialoprotein Homo sapiens 7-10 23261880-8 2013 Density functional theory quantum calculations with an implicit solvent model indicate that Ca(2+) ions are bound most strongly by the phosphorylated serines within BSP, along with reasonably strong binding to Asp and Glu, but weak binding to His and sulfated tyrosine. Serine 150-157 integrin binding sialoprotein Homo sapiens 165-168 23261880-11 2013 The relationships between hydroxyapatite, collagen and BSP are discussed. Durapatite 26-40 integrin binding sialoprotein Homo sapiens 55-58 23141731-0 2012 [Calcium hydroxide regulates bone sialoprotein gene transcription in human dental pulp cells]. Calcium Hydroxide 1-18 integrin binding sialoprotein Homo sapiens 29-46 23141731-1 2012 OBJECTIVE: To analyze the effects of calcium hydroxide [Ca(OH)2] on transcription of the bone sialoprotein (BSP) gene in human dental pulp cells. Calcium Hydroxide 37-54 integrin binding sialoprotein Homo sapiens 89-106 23141731-1 2012 OBJECTIVE: To analyze the effects of calcium hydroxide [Ca(OH)2] on transcription of the bone sialoprotein (BSP) gene in human dental pulp cells. Calcium Hydroxide 37-54 integrin binding sialoprotein Homo sapiens 108-111 23141731-1 2012 OBJECTIVE: To analyze the effects of calcium hydroxide [Ca(OH)2] on transcription of the bone sialoprotein (BSP) gene in human dental pulp cells. Calcium Hydroxide 56-63 integrin binding sialoprotein Homo sapiens 89-106 23141731-1 2012 OBJECTIVE: To analyze the effects of calcium hydroxide [Ca(OH)2] on transcription of the bone sialoprotein (BSP) gene in human dental pulp cells. Calcium Hydroxide 56-63 integrin binding sialoprotein Homo sapiens 108-111 23141731-5 2012 The best dose of Ca(OH)2 on human BSP was determined with the real-time polymerase chain reaction (PCR). Calcium Hydroxide 17-24 integrin binding sialoprotein Homo sapiens 34-37 23141731-11 2012 CONCLUSIONS: This study demonstrate that Ca(OH)2 could stimulate BSP transcription between -84LUC and -868LUC in the human BSP gene promoter in human dental pulp cells. Calcium Hydroxide 41-48 integrin binding sialoprotein Homo sapiens 65-68 23141731-11 2012 CONCLUSIONS: This study demonstrate that Ca(OH)2 could stimulate BSP transcription between -84LUC and -868LUC in the human BSP gene promoter in human dental pulp cells. Calcium Hydroxide 41-48 integrin binding sialoprotein Homo sapiens 123-126 32481839-7 2013 Now we asked the question whether, in the presence of the mineralization activation cocktail, silica induces differentially the fibrillar proteins type I collagen [COLI] and type V collagen [COLV], as well as the non-collagenous proteins alkaline phosphatase [ALP], osteopontin [OPN], osteonectin [ON], osteocalcin [OC], and bone sialoprotein II [BSP]. Silicon Dioxide 94-100 integrin binding sialoprotein Homo sapiens 325-345 22545813-2 2012 Here we proposed to develop a lipid prodrug based on a polyamine analogue bisethylnorspermine (BSP) that can function dually as gene delivery vector and, after intracellular degradation, as active anticancer agent targeting dysregulated polyamine metabolism. N(1),N(11)-diethylnorspermine 74-93 integrin binding sialoprotein Homo sapiens 95-98 22545813-2 2012 Here we proposed to develop a lipid prodrug based on a polyamine analogue bisethylnorspermine (BSP) that can function dually as gene delivery vector and, after intracellular degradation, as active anticancer agent targeting dysregulated polyamine metabolism. Polyamines 237-246 integrin binding sialoprotein Homo sapiens 95-98 22545813-3 2012 We synthesized a prodrug of BSP (LS-BSP) capable of intracellular release of BSP using thiolytically sensitive dithiobenzyl carbamate linker. dithiobenzyl carbamate 111-133 integrin binding sialoprotein Homo sapiens 28-31 22545813-3 2012 We synthesized a prodrug of BSP (LS-BSP) capable of intracellular release of BSP using thiolytically sensitive dithiobenzyl carbamate linker. dithiobenzyl carbamate 111-133 integrin binding sialoprotein Homo sapiens 33-39 22545813-3 2012 We synthesized a prodrug of BSP (LS-BSP) capable of intracellular release of BSP using thiolytically sensitive dithiobenzyl carbamate linker. dithiobenzyl carbamate 111-133 integrin binding sialoprotein Homo sapiens 36-39 21942790-9 2012 RESULTS: The treatment of HCEMs with rh174 upregulated the ALP, OCN, and BSP mRNA levels. hcems 26-31 integrin binding sialoprotein Homo sapiens 73-76 20467657-5 2010 In contrast, unbound BSP is relatively free to migrate between both polar and non-polar regions and the MC form is more readily stabilised by the IL charge via through space interactions and spontaneous movement to charged nano-domains leading to enhancement of the MC lifetime. merocyanine 104-106 integrin binding sialoprotein Homo sapiens 21-24 22192537-8 2012 DFCSs, under the effect of TDM, highly expressed DMP-1 and bone sialoprotein (BSP), indicating their potential for odontogenesis and osteogenesis. dfcss 0-5 integrin binding sialoprotein Homo sapiens 59-76 22192537-8 2012 DFCSs, under the effect of TDM, highly expressed DMP-1 and bone sialoprotein (BSP), indicating their potential for odontogenesis and osteogenesis. dfcss 0-5 integrin binding sialoprotein Homo sapiens 78-81 22201843-9 2012 The poly glutamic acid sequences of BSP act as possible nucleation sites for hydroxyapatite crystals. Polyglutamic Acid 4-22 integrin binding sialoprotein Homo sapiens 36-39 22201843-9 2012 The poly glutamic acid sequences of BSP act as possible nucleation sites for hydroxyapatite crystals. Durapatite 77-91 integrin binding sialoprotein Homo sapiens 36-39 21706355-4 2011 The mRNA expression of ALP was scarcely affected in cells exposed to eugenol, whereas the mRNA and protein expression of BSP was down-regulated depending on the concentrations of eugenol. Eugenol 179-186 integrin binding sialoprotein Homo sapiens 121-124 21650048-8 2011 The results showed BSP gene silence can partial inhibition bone metastasis of breast cancer cells in nude mice by X-ray assay and hematoxylin-eosin staining. Hematoxylin 130-141 integrin binding sialoprotein Homo sapiens 19-22 21650048-8 2011 The results showed BSP gene silence can partial inhibition bone metastasis of breast cancer cells in nude mice by X-ray assay and hematoxylin-eosin staining. Eosine Yellowish-(YS) 142-147 integrin binding sialoprotein Homo sapiens 19-22 20965237-0 2011 cAMP and fibroblast growth factor 2 regulate bone sialoprotein gene expression in human prostate cancer cells. Cyclic AMP 0-4 integrin binding sialoprotein Homo sapiens 45-62 20965237-1 2011 Bone sialoprotein (BSP) is a noncollagenous protein of the extracellular matrix in mineralized connective tissues that has been implicated in the nucleation of hydroxyapatite. Durapatite 160-174 integrin binding sialoprotein Homo sapiens 0-17 20965237-1 2011 Bone sialoprotein (BSP) is a noncollagenous protein of the extracellular matrix in mineralized connective tissues that has been implicated in the nucleation of hydroxyapatite. Durapatite 160-174 integrin binding sialoprotein Homo sapiens 19-22 20965237-4 2011 In human prostate cancer DU145 cells, FSK (1 muM) and FGF2 (10 ng/ml) increased BSP and Runx2 mRNA and protein levels at 3 and 12h, respectively. Colforsin 38-41 integrin binding sialoprotein Homo sapiens 80-83 20965237-6 2011 Treatment of DU145 cells with FSK (1 muM) and FGF2 (10 ng/ml) increased the luciferase activities of constructs between -60LUC to -927LUC and -108LUC to -927LUC, including the human BSP gene promoter. Colforsin 30-33 integrin binding sialoprotein Homo sapiens 182-185 20965237-12 2011 These studies demonstrate that FSK and FGF2 stimulate BSP transcription in DU145 human prostate cancer cells by targeting the CRE1 and CRE2 elements in the human BSP gene promoter. Colforsin 31-34 integrin binding sialoprotein Homo sapiens 54-57 20965237-12 2011 These studies demonstrate that FSK and FGF2 stimulate BSP transcription in DU145 human prostate cancer cells by targeting the CRE1 and CRE2 elements in the human BSP gene promoter. Colforsin 31-34 integrin binding sialoprotein Homo sapiens 162-165 21228489-7 2011 There was a significant increase in the alkaline phosphatase (ALP) activity and protein expression of bone sialoprotein (BSP) and osteocalcin (OC) in response to DEX and DR(DM) as compared to control. Dexamethasone 162-165 integrin binding sialoprotein Homo sapiens 102-119 21228489-7 2011 There was a significant increase in the alkaline phosphatase (ALP) activity and protein expression of bone sialoprotein (BSP) and osteocalcin (OC) in response to DEX and DR(DM) as compared to control. Dexamethasone 162-165 integrin binding sialoprotein Homo sapiens 121-124 21228489-10 2011 The single compound (hederagenin 3-O-(2-O-acetyl)-alpha-L-arabinopyranoside) also significantly increased ALP activity and the level of protein expression of BSP and OC. 3-o-(2-o-acetyl)-alpha-l-arabinopyranoside 33-75 integrin binding sialoprotein Homo sapiens 158-161 20976116-7 2010 The presence of the silica in the silk films influenced osteogenic gene expression, with the upregulation of alkaline phosphatase (ALP), bone sialoprotein (BSP), and collagen type 1 (Col 1) markers. Silicon Dioxide 20-26 integrin binding sialoprotein Homo sapiens 137-154 20976116-7 2010 The presence of the silica in the silk films influenced osteogenic gene expression, with the upregulation of alkaline phosphatase (ALP), bone sialoprotein (BSP), and collagen type 1 (Col 1) markers. Silicon Dioxide 20-26 integrin binding sialoprotein Homo sapiens 156-159 20730931-0 2010 Modification of polymer networks with bone sialoprotein promotes cell attachment and spreading. Polymers 16-23 integrin binding sialoprotein Homo sapiens 38-55 21682547-7 2011 Saos-2 cultured within alginate gels upregulated the osteoblast phenotypic marker genes RUNX2, collagen I (COL1A1) and bone sialoprotein (BSP), and ALP protein activity was highest in alginate gels cast with strontium ions. Alginates 23-31 integrin binding sialoprotein Homo sapiens 119-136 21682547-7 2011 Saos-2 cultured within alginate gels upregulated the osteoblast phenotypic marker genes RUNX2, collagen I (COL1A1) and bone sialoprotein (BSP), and ALP protein activity was highest in alginate gels cast with strontium ions. Alginates 23-31 integrin binding sialoprotein Homo sapiens 138-141 21278525-7 2011 BSP gene expression levels in HBCs with nacre were more intense compared with beta-TCP at weeks 3 and 4. Nacre 40-45 integrin binding sialoprotein Homo sapiens 0-3 21278525-9 2011 Immunocytochemical study revealed that BSP synthesis were presented in the nacre and beta-TCP from week 2 and decreased toward week 4. Nacre 75-80 integrin binding sialoprotein Homo sapiens 39-42 21278525-9 2011 Immunocytochemical study revealed that BSP synthesis were presented in the nacre and beta-TCP from week 2 and decreased toward week 4. beta-tricalcium phosphate 85-93 integrin binding sialoprotein Homo sapiens 39-42 21278525-10 2011 CONCLUSION: This study indicated that nacre promotes ALP, BSP, and OC gene expression. Nacre 38-43 integrin binding sialoprotein Homo sapiens 58-61 20467657-2 2010 SP(Im) was prepared through alkylation of an imidazole to the photoswitchable compound and this derivative was characterised in imidazolium based ILs with increasing chain length to examine the stability of its merocyanine (MC) and spiropyran (SP) forms and compared to standard spiropyran, BSP. spiropyran 0-2 integrin binding sialoprotein Homo sapiens 291-294 20467657-3 2010 The rate of thermal relaxation of the new derivative is found to be about ten times faster than that of BSP as reflected in rates of 13.9 x 10(-3) s(-1) and 1.0 x 10(-3) s(-1) for SP(Im) and BSP, respectively, in [C(6)mIm][NTf(2)]. N-TRIFLURO-ACETYL-BETA-D-GLUCOPYRANOSYLAMINE 223-226 integrin binding sialoprotein Homo sapiens 104-107 20467657-5 2010 In contrast, unbound BSP is relatively free to migrate between both polar and non-polar regions and the MC form is more readily stabilised by the IL charge via through space interactions and spontaneous movement to charged nano-domains leading to enhancement of the MC lifetime. merocyanine 266-268 integrin binding sialoprotein Homo sapiens 21-24 20467657-6 2010 At higher concentrations, rheological and transport properties were investigated to determine the impact of covalent attachment of the BSP fragment to an imidazolium cation on the ionic liquid structure. imidazolium 154-165 integrin binding sialoprotein Homo sapiens 135-138 20565769-8 2010 RESULTS: Dasatinib significantly increased the activity of ALP and the level of calcium deposition in MSC cultured with DAG after, respectively, 7 and 14 days; it upregulated the expression of BSP and OPN genes independently of DAG; and it markedly downregulated the expression of RANKL gene and protein (decrease in RANKL/OPG ratio), the key factor that stimulates osteoclast differentiation and activity. Dasatinib 9-18 integrin binding sialoprotein Homo sapiens 193-196 20438109-0 2010 How does bone sialoprotein promote the nucleation of hydroxyapatite? Durapatite 53-67 integrin binding sialoprotein Homo sapiens 9-26 20438109-3 2010 Although BSP has been proposed to be a nucleator of hydroxyapatite (Ca(5)(PO(4))(3)OH), the major mineral component of bone, no detailed mechanism for the nucleation process has been elucidated at the atomic level to date. Durapatite 52-66 integrin binding sialoprotein Homo sapiens 9-12 20438109-3 2010 Although BSP has been proposed to be a nucleator of hydroxyapatite (Ca(5)(PO(4))(3)OH), the major mineral component of bone, no detailed mechanism for the nucleation process has been elucidated at the atomic level to date. ca(5) 68-73 integrin binding sialoprotein Homo sapiens 9-12 20438109-3 2010 Although BSP has been proposed to be a nucleator of hydroxyapatite (Ca(5)(PO(4))(3)OH), the major mineral component of bone, no detailed mechanism for the nucleation process has been elucidated at the atomic level to date. Polonium 74-77 integrin binding sialoprotein Homo sapiens 9-12 20438109-4 2010 In the present work, using a peptide model, we apply molecular dynamics (MD) simulations to study the conformational effect of a proposed nucleating motif of BSP (a phosphorylated, acidic, 10 amino-acid residue sequence) on controlling the distributions of Ca(2+) and inorganic phosphate (Pi) ions in solution, and specifically, we explore whether a nucleating template for orientated hydroxyapatite could be formed in different peptide conformations. Phosphates 278-287 integrin binding sialoprotein Homo sapiens 158-161 20438109-4 2010 In the present work, using a peptide model, we apply molecular dynamics (MD) simulations to study the conformational effect of a proposed nucleating motif of BSP (a phosphorylated, acidic, 10 amino-acid residue sequence) on controlling the distributions of Ca(2+) and inorganic phosphate (Pi) ions in solution, and specifically, we explore whether a nucleating template for orientated hydroxyapatite could be formed in different peptide conformations. Durapatite 385-399 integrin binding sialoprotein Homo sapiens 158-161 20438109-8 2010 Therefore, independent of conformations, the BSP nucleating motif is more likely to help nucleate an amorphous calcium phosphate cluster, which ultimately converts to crystalline hydroxyapatite. calcium phosphate 111-128 integrin binding sialoprotein Homo sapiens 45-48 20438109-8 2010 Therefore, independent of conformations, the BSP nucleating motif is more likely to help nucleate an amorphous calcium phosphate cluster, which ultimately converts to crystalline hydroxyapatite. Durapatite 179-193 integrin binding sialoprotein Homo sapiens 45-48 19386107-2 2009 This model is particularly relevant to cancer cell metastasis to bone since BSP, bound to the alphavbeta3 integrin through its arginine-glycine-aspartic acid motif, could recruit MMP-2 to the cell surface. Arginine 127-135 integrin binding sialoprotein Homo sapiens 76-79 19671866-2 2009 Previous studies have separately reported elevated expression of BSP, OPN, or DSPP in prostate tumor paraffin sections. Paraffin 101-109 integrin binding sialoprotein Homo sapiens 65-68 19386107-2 2009 This model is particularly relevant to cancer cell metastasis to bone since BSP, bound to the alphavbeta3 integrin through its arginine-glycine-aspartic acid motif, could recruit MMP-2 to the cell surface. Aspartic Acid 144-157 integrin binding sialoprotein Homo sapiens 76-79 19402137-2 2010 Moreover, we tested whether synthetic polymer-based porous scaffolds, despite the absence of a mineral component, could support ectopic bone formation by human BMSC if coated with BSP. Polymers 38-45 integrin binding sialoprotein Homo sapiens 180-183 19402137-3 2010 Adsorption of recombinant human BSP on tissue culture-treated polystyrene (TCTP), beta-tricalcium phosphate (Osteologic) or synthetic polymer (Polyactive) substrates was dose dependent, but did not consistently accelerate or enhance in vitro BMSC osteogenic differentiation, as assessed by the mRNA expression of osteoblast-related genes. Polystyrenes 62-73 integrin binding sialoprotein Homo sapiens 32-35 19402137-3 2010 Adsorption of recombinant human BSP on tissue culture-treated polystyrene (TCTP), beta-tricalcium phosphate (Osteologic) or synthetic polymer (Polyactive) substrates was dose dependent, but did not consistently accelerate or enhance in vitro BMSC osteogenic differentiation, as assessed by the mRNA expression of osteoblast-related genes. Tetrachlorothiophene 75-79 integrin binding sialoprotein Homo sapiens 32-35 19402137-3 2010 Adsorption of recombinant human BSP on tissue culture-treated polystyrene (TCTP), beta-tricalcium phosphate (Osteologic) or synthetic polymer (Polyactive) substrates was dose dependent, but did not consistently accelerate or enhance in vitro BMSC osteogenic differentiation, as assessed by the mRNA expression of osteoblast-related genes. beta-tricalcium phosphate 82-107 integrin binding sialoprotein Homo sapiens 32-35 19402137-3 2010 Adsorption of recombinant human BSP on tissue culture-treated polystyrene (TCTP), beta-tricalcium phosphate (Osteologic) or synthetic polymer (Polyactive) substrates was dose dependent, but did not consistently accelerate or enhance in vitro BMSC osteogenic differentiation, as assessed by the mRNA expression of osteoblast-related genes. Polymers 134-141 integrin binding sialoprotein Homo sapiens 32-35 20074475-4 2009 In our study, the immunohistochemical expression of BSP, VEGF, eNOS in eAB samples was significantly higher (p < 0.05) compared to values recorded in iAB and hB samples. eab 71-74 integrin binding sialoprotein Homo sapiens 52-55 19442631-6 2009 Activation of endogenous canonical Wnt signaling with LiCl suppressed alkaline phosphatase (ALP) activity and expression of genes associated with cementum function; ALP, bone sialoprotein (BSP), and osteocalcin (OCN). Lithium Chloride 54-58 integrin binding sialoprotein Homo sapiens 170-187 19442631-6 2009 Activation of endogenous canonical Wnt signaling with LiCl suppressed alkaline phosphatase (ALP) activity and expression of genes associated with cementum function; ALP, bone sialoprotein (BSP), and osteocalcin (OCN). Lithium Chloride 54-58 integrin binding sialoprotein Homo sapiens 189-192 19386107-2 2009 This model is particularly relevant to cancer cell metastasis to bone since BSP, bound to the alphavbeta3 integrin through its arginine-glycine-aspartic acid motif, could recruit MMP-2 to the cell surface. Glycine 136-143 integrin binding sialoprotein Homo sapiens 76-79 19012272-10 2009 We found an increase of ALP as well as BSPII expression for osteogenic-induced hBMSCs on alginate-gelatin-HA scaffolds. Alginates 89-97 integrin binding sialoprotein Homo sapiens 39-44 19127545-0 2009 Parathyroid hormone regulation of the human bone sialoprotein gene transcription is mediated through two cAMP response elements. Cyclic AMP 105-109 integrin binding sialoprotein Homo sapiens 44-61 19127545-3 2009 We here report that two cAMP response elements (CRE) in the human BSP gene promoter are target of PTH. Cyclic AMP 24-28 integrin binding sialoprotein Homo sapiens 66-69 18729384-3 2008 The 26 amino acid domain encoded by exon 4 of BSP is shown by a series of binding and activity assays to be involved in the displacement of MMP-2"s propeptide from the active site and thereby inducing the protease activity. propeptide 148-158 integrin binding sialoprotein Homo sapiens 46-49 18782614-7 2008 Sulfobromophthalein (BSP) was used as a marker for MRP2 and OATP-B functionality. Sulfobromophthalein 0-19 integrin binding sialoprotein Homo sapiens 21-24 18406397-8 2008 Following the addition of 10(-4)M BA, the expression levels of BSP, OPN, and OPG increased, whereas the expression levels of type I collagen and M-CSF were not markedly affected. Barium 34-36 integrin binding sialoprotein Homo sapiens 63-66 18406397-9 2008 CONCLUSION: These results suggest that BA stimulates bone formation by increasing the production of BSP and OPN, whereas it suppresses osteoclast differentiation by increasing the production of OPG by human osteoblasts. Barium 39-41 integrin binding sialoprotein Homo sapiens 100-103 18620053-0 2008 Bone sialoprotein-collagen interaction promotes hydroxyapatite nucleation. Durapatite 48-62 integrin binding sialoprotein Homo sapiens 0-17 17884722-11 2007 Although expression of bone sialo protein (BSP) at the mRNA level increased both after 24h and 48 h in the presence of manganese, no increased expression of BSP was detected at the protein level. Manganese 119-128 integrin binding sialoprotein Homo sapiens 23-41 18729384-1 2008 Bone sialoprotein (BSP) has been shown to induce limited gelatinase activity in latent matrix metalloproteinase-2 (MMP-2) without removal of the propeptide and to restore enzymatic activity to MMP-2 previously inhibited by tissue inhibitor of matrix metalloproteinase-2 (TIMP2). propeptide 145-155 integrin binding sialoprotein Homo sapiens 0-23 18088579-4 2008 BSP human promoter deletion analyses delineated a -56/-84 region, which comprises a cAMP response element (CRE) that was sufficient for maximal promoter activity in breast cancer cell lines. Cyclic AMP 84-88 integrin binding sialoprotein Homo sapiens 0-3 17884722-11 2007 Although expression of bone sialo protein (BSP) at the mRNA level increased both after 24h and 48 h in the presence of manganese, no increased expression of BSP was detected at the protein level. Manganese 119-128 integrin binding sialoprotein Homo sapiens 43-46 16488077-4 2007 The median BSP mRNA levels were 6.1 and 0.9copies/microl cDNA in PDAC and CP tissues, respectively, and zero copies/microl cDNA in normal pancreatic tissues. pdac 65-69 integrin binding sialoprotein Homo sapiens 11-14 16488077-6 2007 BSP was localized in the tubular complexes of both CP and PDAC, as well as in pancreatic cancer cells. pdac 58-62 integrin binding sialoprotein Homo sapiens 0-3 17096901-6 2006 The results showed that the HLA-A*0201-BSP fusion protein was successfully expressed in the form of inclusion body and amounted to over 28% of total cell proteins via induction at 42 degrees C. After washed with triton X-100 and urea, the inclusion body was dissolved with 8 mol/L urea and then purified with Sepharcyl S-300 HR, and the final purity reached above 90%. Urea 229-233 integrin binding sialoprotein Homo sapiens 39-42 17917883-6 2007 The treatment induced extracellular calcium deposition and gene and protein expression of osteonectin (ON) and bone sialoprotein (BSP): beta-GlyP induced an increase (c. 2.2-fold) of the ON gene and dexa augmented (c. 2.7-fold) the gene expression of BSP II. beta-glycerophosphoric acid 136-145 integrin binding sialoprotein Homo sapiens 111-128 17917883-6 2007 The treatment induced extracellular calcium deposition and gene and protein expression of osteonectin (ON) and bone sialoprotein (BSP): beta-GlyP induced an increase (c. 2.2-fold) of the ON gene and dexa augmented (c. 2.7-fold) the gene expression of BSP II. beta-glycerophosphoric acid 136-145 integrin binding sialoprotein Homo sapiens 130-133 17917883-6 2007 The treatment induced extracellular calcium deposition and gene and protein expression of osteonectin (ON) and bone sialoprotein (BSP): beta-GlyP induced an increase (c. 2.2-fold) of the ON gene and dexa augmented (c. 2.7-fold) the gene expression of BSP II. beta-glycerophosphoric acid 136-145 integrin binding sialoprotein Homo sapiens 251-257 17966928-9 2007 The SE-related increase of BSP in progenitor cells indicates an earlier differentiation of immigrated cells and could thus explain earlier implant integration and shorter time to functional loading observed in the clinic. Selenium 4-6 integrin binding sialoprotein Homo sapiens 27-30 17096901-6 2006 The results showed that the HLA-A*0201-BSP fusion protein was successfully expressed in the form of inclusion body and amounted to over 28% of total cell proteins via induction at 42 degrees C. After washed with triton X-100 and urea, the inclusion body was dissolved with 8 mol/L urea and then purified with Sepharcyl S-300 HR, and the final purity reached above 90%. Urea 281-285 integrin binding sialoprotein Homo sapiens 39-42 17096901-6 2006 The results showed that the HLA-A*0201-BSP fusion protein was successfully expressed in the form of inclusion body and amounted to over 28% of total cell proteins via induction at 42 degrees C. After washed with triton X-100 and urea, the inclusion body was dissolved with 8 mol/L urea and then purified with Sepharcyl S-300 HR, and the final purity reached above 90%. sepharcyl s 309-320 integrin binding sialoprotein Homo sapiens 39-42 16313952-3 2006 We found that biaxially oriented polypropylene (BOPP) plasma treated in ammonia significantly reduced up-regulation of expression of osteogenic marker genes, such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). Polypropylenes 33-46 integrin binding sialoprotein Homo sapiens 194-211 16313952-3 2006 We found that biaxially oriented polypropylene (BOPP) plasma treated in ammonia significantly reduced up-regulation of expression of osteogenic marker genes, such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). PyBOP 48-52 integrin binding sialoprotein Homo sapiens 213-216 17096901-3 2006 The extracellular region HLA*0201 was cloned by RT-PCR from HLA-A2(+) donor, and a 15-amino acid biotin-protein ligase (BirA) substrate peptide (BSP) for BirA-dependent biotinylation was added to the COOH-terminus of HLA-A*0201 heavy chain. Carbonic Acid 200-204 integrin binding sialoprotein Homo sapiens 145-148 17096901-6 2006 The results showed that the HLA-A*0201-BSP fusion protein was successfully expressed in the form of inclusion body and amounted to over 28% of total cell proteins via induction at 42 degrees C. After washed with triton X-100 and urea, the inclusion body was dissolved with 8 mol/L urea and then purified with Sepharcyl S-300 HR, and the final purity reached above 90%. Octoxynol 212-224 integrin binding sialoprotein Homo sapiens 39-42 16313952-3 2006 We found that biaxially oriented polypropylene (BOPP) plasma treated in ammonia significantly reduced up-regulation of expression of osteogenic marker genes, such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). Polypropylenes 33-46 integrin binding sialoprotein Homo sapiens 213-216 16313952-3 2006 We found that biaxially oriented polypropylene (BOPP) plasma treated in ammonia significantly reduced up-regulation of expression of osteogenic marker genes, such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). Ammonia 72-79 integrin binding sialoprotein Homo sapiens 194-211 16313952-3 2006 We found that biaxially oriented polypropylene (BOPP) plasma treated in ammonia significantly reduced up-regulation of expression of osteogenic marker genes, such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). PyBOP 48-52 integrin binding sialoprotein Homo sapiens 194-211 16313952-3 2006 We found that biaxially oriented polypropylene (BOPP) plasma treated in ammonia significantly reduced up-regulation of expression of osteogenic marker genes, such as alkaline phosphatase (ALP), bone sialoprotein (BSP) and osteocalcin (OC). Ammonia 72-79 integrin binding sialoprotein Homo sapiens 213-216 16313952-6 2006 BSP was expressed weakly on day 3, but was up-regulated when cultured on Ny-t and Ny-p. Its expression reached its maximum on day 14 when cultured on a polystyrene control, while it was cyclically up-regulated on Ny-t. Polystyrenes 152-163 integrin binding sialoprotein Homo sapiens 0-3 12798071-2 2003 We have previously reported that osteogenic differentiation of human marrow stroma-derived mesenchymal stem cells (hMSCs) is suppressed upon exposure to titanium particles, accompanied by reduced bone sialoprotein (BSP) gene expression, diminished production of collagen type I and BSP, decreased cellular viability and proliferation, and inhibition of extracellular matrix mineralization. Titanium 153-161 integrin binding sialoprotein Homo sapiens 196-213 15892946-6 2005 The codon usage patterns for the amino acid serine reveal a bias for AGC and AGT codons within the human genes dspp, dmp1 and bsp, mouse dspp and dmp1 but not significantly for statherin or caseins. amino acid serine 33-50 integrin binding sialoprotein Homo sapiens 126-129 15004030-1 2004 Addition of an organophosphate source to UMR osteoblastic cultures activates a mineralization program in which BSP localizes to extracellular matrix sites where hydroxyapatite crystals are subsequently nucleated. Organophosphates 15-30 integrin binding sialoprotein Homo sapiens 111-114 15004030-1 2004 Addition of an organophosphate source to UMR osteoblastic cultures activates a mineralization program in which BSP localizes to extracellular matrix sites where hydroxyapatite crystals are subsequently nucleated. Durapatite 161-175 integrin binding sialoprotein Homo sapiens 111-114 15004030-5 2004 After organophosphate addition, BSP accumulates within these BAG-75-containing BMF precursors, with hydroxyapatite crystal nucleation occurring subsequently. Organophosphates 6-21 integrin binding sialoprotein Homo sapiens 32-35 15004030-5 2004 After organophosphate addition, BSP accumulates within these BAG-75-containing BMF precursors, with hydroxyapatite crystal nucleation occurring subsequently. BMF 79-82 integrin binding sialoprotein Homo sapiens 32-35 15067347-6 2004 The antibody against human BSP II was significantly more active than all ASOs used and was equally active or even surpassed the activity of ibandronate and ErPC3 in all three assays. Ibandronic Acid 140-151 integrin binding sialoprotein Homo sapiens 27-33 14506948-6 2003 BSP protein expression was evaluated by the immunophosphatase technique using a BSP polyclonal antibody in paraffin-embedded cervical biopsies. Paraffin 107-115 integrin binding sialoprotein Homo sapiens 0-3 16055156-9 2005 This study demonstrates that an optimal compressive force stimulates in vitro mineralization by BSP synthesis through the autocrin action of PGE(2) production. Dinoprostone 141-147 integrin binding sialoprotein Homo sapiens 96-99 15795688-3 2005 The expression of BSP coincides with initial bone mineralization and is believed to be a center of crystallization for hydroxyapatite formation. Durapatite 119-133 integrin binding sialoprotein Homo sapiens 18-21 15324309-3 2004 We hypothesized that ZOL (zoledronic acid), a potent third-generation BP, may induce the expression of proteins associated with the bone-forming potential of osteoblastic cells such as BSP (bone sialo-protein). Zoledronic Acid 21-24 integrin binding sialoprotein Homo sapiens 185-188 15324309-3 2004 We hypothesized that ZOL (zoledronic acid), a potent third-generation BP, may induce the expression of proteins associated with the bone-forming potential of osteoblastic cells such as BSP (bone sialo-protein). Zoledronic Acid 21-24 integrin binding sialoprotein Homo sapiens 190-208 15324309-3 2004 We hypothesized that ZOL (zoledronic acid), a potent third-generation BP, may induce the expression of proteins associated with the bone-forming potential of osteoblastic cells such as BSP (bone sialo-protein). Zoledronic Acid 26-41 integrin binding sialoprotein Homo sapiens 185-188 15324309-3 2004 We hypothesized that ZOL (zoledronic acid), a potent third-generation BP, may induce the expression of proteins associated with the bone-forming potential of osteoblastic cells such as BSP (bone sialo-protein). Zoledronic Acid 26-41 integrin binding sialoprotein Homo sapiens 190-208 15324309-3 2004 We hypothesized that ZOL (zoledronic acid), a potent third-generation BP, may induce the expression of proteins associated with the bone-forming potential of osteoblastic cells such as BSP (bone sialo-protein). Diphosphonates 70-72 integrin binding sialoprotein Homo sapiens 185-188 15324309-3 2004 We hypothesized that ZOL (zoledronic acid), a potent third-generation BP, may induce the expression of proteins associated with the bone-forming potential of osteoblastic cells such as BSP (bone sialo-protein). Diphosphonates 70-72 integrin binding sialoprotein Homo sapiens 190-208 15324309-6 2004 Nuclear run-on and mRNA decay assays showed no effect at the transcriptional level but a stabilization of BSP transcripts in ZOL-treated cells. Zoledronic Acid 125-128 integrin binding sialoprotein Homo sapiens 106-109 15324309-7 2004 ZOL effect on BSP expression occurred through an interference with the mevalonate pathway since it was reversed by either mevalonate pathway intermediates or a Rho GTPase activator. Mevalonic Acid 71-81 integrin binding sialoprotein Homo sapiens 14-17 15324309-7 2004 ZOL effect on BSP expression occurred through an interference with the mevalonate pathway since it was reversed by either mevalonate pathway intermediates or a Rho GTPase activator. Mevalonic Acid 122-132 integrin binding sialoprotein Homo sapiens 14-17 15324309-10 2004 Our study demonstrates that ZOL induces BSP expression in osteoblast-like cells through inactivation of Rho GTPases and provides a potential mechanism to explain the favourable effects of ZOL treatment on bone mass and integrity. Zoledronic Acid 28-31 integrin binding sialoprotein Homo sapiens 40-43 15324309-10 2004 Our study demonstrates that ZOL induces BSP expression in osteoblast-like cells through inactivation of Rho GTPases and provides a potential mechanism to explain the favourable effects of ZOL treatment on bone mass and integrity. Zoledronic Acid 188-191 integrin binding sialoprotein Homo sapiens 40-43 15549369-12 2004 Blood lactate was higher in the hot trial compared with the control trial at 5 min post-SpII ( P<0.025). Lactic Acid 6-13 integrin binding sialoprotein Homo sapiens 88-92 15499184-2 2004 The recently identified organic anion transporter family OATP (organic anion transporting polypeptide)/LST (liver-specific transporter) family, transport bile acids, hormones as well as eicosanoids, various compounds (BSP, HMG-CoA reductase inhibitor, angiotensin converting enzyme inhibitor, etc.). Bile Acids and Salts 154-164 integrin binding sialoprotein Homo sapiens 218-221 12798071-2 2003 We have previously reported that osteogenic differentiation of human marrow stroma-derived mesenchymal stem cells (hMSCs) is suppressed upon exposure to titanium particles, accompanied by reduced bone sialoprotein (BSP) gene expression, diminished production of collagen type I and BSP, decreased cellular viability and proliferation, and inhibition of extracellular matrix mineralization. Titanium 153-161 integrin binding sialoprotein Homo sapiens 215-218 12798071-2 2003 We have previously reported that osteogenic differentiation of human marrow stroma-derived mesenchymal stem cells (hMSCs) is suppressed upon exposure to titanium particles, accompanied by reduced bone sialoprotein (BSP) gene expression, diminished production of collagen type I and BSP, decreased cellular viability and proliferation, and inhibition of extracellular matrix mineralization. Titanium 153-161 integrin binding sialoprotein Homo sapiens 282-285 12952202-2 2003 The in vitro phosphorylation sites were determined by 32P-labeling of native BSP using purified casein kinase II (CKII), followed by peptide mapping and solid-phase N-terminal sequence analyses. Phosphorus-32 54-57 integrin binding sialoprotein Homo sapiens 77-80 12952202-4 2003 Native BSP incorporated approximately 2.5 mol of phosphate/mol of BSP by CKII, which were distributed over four major peptide peaks and three shoulder peaks within the peptide map with varying degrees of phosphorylation. Phosphates 49-58 integrin binding sialoprotein Homo sapiens 7-10 12952202-5 2003 Further studies using the [14C] CM-DTT thiol reagent indicated that native and deglycosylated BSP incorporated 5.84 and 5.80 mol of 14C/mol of BSP, respectively. Carbon-14 27-30 integrin binding sialoprotein Homo sapiens 94-97 12952202-5 2003 Further studies using the [14C] CM-DTT thiol reagent indicated that native and deglycosylated BSP incorporated 5.84 and 5.80 mol of 14C/mol of BSP, respectively. Dithiothreitol 35-38 integrin binding sialoprotein Homo sapiens 94-97 12952202-5 2003 Further studies using the [14C] CM-DTT thiol reagent indicated that native and deglycosylated BSP incorporated 5.84 and 5.80 mol of 14C/mol of BSP, respectively. Sulfhydryl Compounds 39-44 integrin binding sialoprotein Homo sapiens 94-97 12952202-5 2003 Further studies using the [14C] CM-DTT thiol reagent indicated that native and deglycosylated BSP incorporated 5.84 and 5.80 mol of 14C/mol of BSP, respectively. Carbon-14 132-135 integrin binding sialoprotein Homo sapiens 94-97 12952202-6 2003 This confirmed that there were approximately 5.8 mol P-Ser/mol of BSP naturally (in vivo) occurring phosphorylation sites and that there was no overlap between the phosphorylation and glycosylation sites. Serine 55-58 integrin binding sialoprotein Homo sapiens 66-69 12952202-7 2003 The 5.8 mol P-Ser/mol BSP reflects the total number of mols of naturally occurring phosphorylation, phosphorylated in vivo by CKII (4.1 mol), protein kinase C (0.9 mol), and cGMP-dependent kinase (0.8 mol). Serine 14-17 integrin binding sialoprotein Homo sapiens 22-25 12472226-5 2002 Exposure of OS-treated hMSCs to submicron commercially pure titanium (cpTi) particles suppresses BSP gene expression, reduces collagen type I and BSP production, decreases cellular proliferation and viability, and inhibits matrix mineralization. Osmium 12-14 integrin binding sialoprotein Homo sapiens 97-100 12472226-5 2002 Exposure of OS-treated hMSCs to submicron commercially pure titanium (cpTi) particles suppresses BSP gene expression, reduces collagen type I and BSP production, decreases cellular proliferation and viability, and inhibits matrix mineralization. Osmium 12-14 integrin binding sialoprotein Homo sapiens 146-149 12472226-5 2002 Exposure of OS-treated hMSCs to submicron commercially pure titanium (cpTi) particles suppresses BSP gene expression, reduces collagen type I and BSP production, decreases cellular proliferation and viability, and inhibits matrix mineralization. Titanium 60-68 integrin binding sialoprotein Homo sapiens 97-100 12472226-5 2002 Exposure of OS-treated hMSCs to submicron commercially pure titanium (cpTi) particles suppresses BSP gene expression, reduces collagen type I and BSP production, decreases cellular proliferation and viability, and inhibits matrix mineralization. Titanium 60-68 integrin binding sialoprotein Homo sapiens 146-149 11669636-4 2001 Thus, the proposed role of BSP in hydroxyapatite nucleation and growth may depend on such modifying groups. Durapatite 34-48 integrin binding sialoprotein Homo sapiens 27-30 12459268-1 2002 Bone sialoprotein (BSP) is a phosphorylated and sulphated glycoprotein with hydroxyapatite nucleating properties that is specifically expressed in association with physiological and pathological mineralization. Durapatite 76-90 integrin binding sialoprotein Homo sapiens 0-17 12459268-1 2002 Bone sialoprotein (BSP) is a phosphorylated and sulphated glycoprotein with hydroxyapatite nucleating properties that is specifically expressed in association with physiological and pathological mineralization. Durapatite 76-90 integrin binding sialoprotein Homo sapiens 19-22 12162498-8 2002 U0126, a specific inhibitor of MEK-1/2 members of the MAPK family, abolished BMP-2-mediated expression of BSP and OCN. U 0126 0-5 integrin binding sialoprotein Homo sapiens 106-109 12023890-6 2002 Peak levels of mols of P-Ser/mol of BSP coincided with maximum rates of Ca-P deposition in calvarial implants. calcium phosphate 72-76 integrin binding sialoprotein Homo sapiens 36-39 12023890-9 2002 Further evaluation of the data provides the first evidence of a direct and linear relationship between the rate of Ca-P deposition and the ratio of P-Ser-BSP/P-Ser-OPN for calvarial implants. calcium phosphate 115-119 integrin binding sialoprotein Homo sapiens 154-157 12023890-9 2002 Further evaluation of the data provides the first evidence of a direct and linear relationship between the rate of Ca-P deposition and the ratio of P-Ser-BSP/P-Ser-OPN for calvarial implants. Serine 150-153 integrin binding sialoprotein Homo sapiens 154-157 11669636-6 2001 This work combines matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry with selective enzyme treatment of BSP to provide new information on the precise distribution and structure of oligosaccharides, sulfate, and phosphate groups in BSP isolated from human bone. Oligosaccharides 219-235 integrin binding sialoprotein Homo sapiens 143-146 11669636-6 2001 This work combines matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry with selective enzyme treatment of BSP to provide new information on the precise distribution and structure of oligosaccharides, sulfate, and phosphate groups in BSP isolated from human bone. Sulfates 237-244 integrin binding sialoprotein Homo sapiens 143-146 11669636-6 2001 This work combines matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry with selective enzyme treatment of BSP to provide new information on the precise distribution and structure of oligosaccharides, sulfate, and phosphate groups in BSP isolated from human bone. Phosphates 250-259 integrin binding sialoprotein Homo sapiens 143-146 11459848-7 2001 Carbohydrate analysis revealed 10 different complex-type N-glycans on both proteins and eight different O-glycans on recombinant BSP, four of those were found on bone-derived BSP. Carbohydrates 0-12 integrin binding sialoprotein Homo sapiens 175-178 11459848-7 2001 Carbohydrate analysis revealed 10 different complex-type N-glycans on both proteins and eight different O-glycans on recombinant BSP, four of those were found on bone-derived BSP. o-glycans 104-113 integrin binding sialoprotein Homo sapiens 129-132 11459848-11 2001 The affinity for hydroxyapatite was higher for bone-derived BSP than for recombinant BSP. Durapatite 17-31 integrin binding sialoprotein Homo sapiens 60-63 11459848-11 2001 The affinity for hydroxyapatite was higher for bone-derived BSP than for recombinant BSP. Durapatite 17-31 integrin binding sialoprotein Homo sapiens 85-88 11696986-8 2001 Poly(Glu) was able to displace a maximum of 100% of Poly(Glu), 81% of OPN, 68% of BSP and 65% of Poly(Asp). poly 0-4 integrin binding sialoprotein Homo sapiens 82-85 11696986-0 2001 Binding of bone sialoprotein, osteopontin and synthetic polypeptides to hydroxyapatite. Durapatite 72-86 integrin binding sialoprotein Homo sapiens 11-28 11696986-8 2001 Poly(Glu) was able to displace a maximum of 100% of Poly(Glu), 81% of OPN, 68% of BSP and 65% of Poly(Asp). Glutamic Acid 5-8 integrin binding sialoprotein Homo sapiens 82-85 11696986-1 2001 The phosphorylated acidic glycoproteins bone sialoprotein (BSP) and osteopontin (OPN) bind to hydroxyapatite (HA) crystals and may be involved in the regulation of bone mineralization. Durapatite 94-108 integrin binding sialoprotein Homo sapiens 40-57 11696986-8 2001 Poly(Glu) was able to displace a maximum of 100% of Poly(Glu), 81% of OPN, 68% of BSP and 65% of Poly(Asp). poly(glu) 0-9 integrin binding sialoprotein Homo sapiens 82-85 11696986-1 2001 The phosphorylated acidic glycoproteins bone sialoprotein (BSP) and osteopontin (OPN) bind to hydroxyapatite (HA) crystals and may be involved in the regulation of bone mineralization. Durapatite 94-108 integrin binding sialoprotein Homo sapiens 59-62 11696986-1 2001 The phosphorylated acidic glycoproteins bone sialoprotein (BSP) and osteopontin (OPN) bind to hydroxyapatite (HA) crystals and may be involved in the regulation of bone mineralization. Durapatite 110-112 integrin binding sialoprotein Homo sapiens 40-57 11696986-1 2001 The phosphorylated acidic glycoproteins bone sialoprotein (BSP) and osteopontin (OPN) bind to hydroxyapatite (HA) crystals and may be involved in the regulation of bone mineralization. Durapatite 110-112 integrin binding sialoprotein Homo sapiens 59-62 11696986-2 2001 The HA-binding properties of these proteins have been attributed to glutamic acid-rich sequences in BSP and aspartic acid-rich sequences in OPN. Glutamic Acid 68-81 integrin binding sialoprotein Homo sapiens 100-103 11696986-9 2001 Poly(Asp) was able to displace a maximum of 100% of Poly(Glu), 99% of Poly(Asp), 95% of OPN and 89% of BSP. poly 0-4 integrin binding sialoprotein Homo sapiens 103-106 11696986-3 2001 The present study examines the roles of these polycarboxylate sequences in the binding of BSP and OPN to HA. polycarboxylate 46-61 integrin binding sialoprotein Homo sapiens 90-93 11696986-9 2001 Poly(Asp) was able to displace a maximum of 100% of Poly(Glu), 99% of Poly(Asp), 95% of OPN and 89% of BSP. Aspartic Acid 5-9 integrin binding sialoprotein Homo sapiens 103-106 11696986-7 2001 To investigate the role of glutamic acid-rich and aspartic acid-rich sequences in the binding to HA of BSP and OPN, respectively, competitive binding studies with Poly(Glu) and Poly(Asp) were performed. Glutamic Acid 27-40 integrin binding sialoprotein Homo sapiens 103-106 11696986-9 2001 Poly(Asp) was able to displace a maximum of 100% of Poly(Glu), 99% of Poly(Asp), 95% of OPN and 89% of BSP. Aspartic Acid 5-8 integrin binding sialoprotein Homo sapiens 103-106 11696986-7 2001 To investigate the role of glutamic acid-rich and aspartic acid-rich sequences in the binding to HA of BSP and OPN, respectively, competitive binding studies with Poly(Glu) and Poly(Asp) were performed. Aspartic Acid 50-63 integrin binding sialoprotein Homo sapiens 103-106 11696986-10 2001 These results are consistent with the view that BSP and OPN bind to HA via their polycarboxylate sequences, but suggest a complex mode of interaction between polyelectrolytes and ionic crystals. polycarboxylate 81-96 integrin binding sialoprotein Homo sapiens 48-51 11763409-8 2001 Significant positive correlations exist between serum BSP and biomarkers of bone resorption (Pyr,DPyr,NTX) as well as bone resorptive cytokines (IL-11,TGFbeta2). pyr,dpyr,ntx 93-105 integrin binding sialoprotein Homo sapiens 54-57 11108282-7 2000 In addition, the PKA pathway inhibitor (9-(2-tetrahydrofuryl) adenine (THFA) partially, but significantly reversed the PTHrP-mediated down-regulation of BSP gene expression. 9-(2-tetrahydrofuryl) adenine 40-69 integrin binding sialoprotein Homo sapiens 153-156 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. arginine-glycine 94-110 integrin binding sialoprotein Homo sapiens 10-27 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. arginine-glycine 94-110 integrin binding sialoprotein Homo sapiens 29-32 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. Aspartic Acid 111-124 integrin binding sialoprotein Homo sapiens 10-27 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. Aspartic Acid 111-124 integrin binding sialoprotein Homo sapiens 29-32 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. Glutamic Acid 168-181 integrin binding sialoprotein Homo sapiens 10-27 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. Glutamic Acid 168-181 integrin binding sialoprotein Homo sapiens 29-32 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. Glutamic Acid 168-171 integrin binding sialoprotein Homo sapiens 10-27 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. Glutamic Acid 168-171 integrin binding sialoprotein Homo sapiens 29-32 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. Glutamic Acid 183-186 integrin binding sialoprotein Homo sapiens 10-27 11113390-1 2000 Mammalian bone sialoprotein (BSP) is a mineralized tissue-specific protein containing an RGD (arginine-glycine-aspartic acid) cell-attachment sequence and two distinct glutamic acid (glu)-rich regions, with each containing one contiguous glu sequence. Glutamic Acid 183-186 integrin binding sialoprotein Homo sapiens 29-32 11113390-3 2000 To further delineate the domains responsible for these activities, porcine BSP cDNA was used to construct expression vectors coding for two partial-length recombinant BSP peptides: P2S (residues 42-87), containing the first glutamic acid-rich domain; and P1L (residues 69-300), containing the second glutamic acid-rich region and the RGD sequence. Glutamic Acid 224-237 integrin binding sialoprotein Homo sapiens 75-78 11113390-3 2000 To further delineate the domains responsible for these activities, porcine BSP cDNA was used to construct expression vectors coding for two partial-length recombinant BSP peptides: P2S (residues 42-87), containing the first glutamic acid-rich domain; and P1L (residues 69-300), containing the second glutamic acid-rich region and the RGD sequence. Glutamic Acid 300-313 integrin binding sialoprotein Homo sapiens 75-78 11108282-7 2000 In addition, the PKA pathway inhibitor (9-(2-tetrahydrofuryl) adenine (THFA) partially, but significantly reversed the PTHrP-mediated down-regulation of BSP gene expression. TETRAHYDROFURFURYL ALCOHOL 71-75 integrin binding sialoprotein Homo sapiens 153-156 11108282-8 2000 Furthermore, THFA alone significantly increased BSP messenger RNA (mRNA) expression in cementoblasts. TETRAHYDROFURFURYL ALCOHOL 13-17 integrin binding sialoprotein Homo sapiens 48-51 11108282-11 2000 These data indicate that the cAMP/PKA pathway mediates the PTHrP-mediated down-regulation of BSP mRNA expression in cementoblasts; and furthermore, this pathway may, through an intrinsic inhibition mechanism, regulate the basal level of BSP mRNA expression. Cyclic AMP 29-33 integrin binding sialoprotein Homo sapiens 93-96 11108282-11 2000 These data indicate that the cAMP/PKA pathway mediates the PTHrP-mediated down-regulation of BSP mRNA expression in cementoblasts; and furthermore, this pathway may, through an intrinsic inhibition mechanism, regulate the basal level of BSP mRNA expression. Cyclic AMP 29-33 integrin binding sialoprotein Homo sapiens 237-240 11314517-9 2000 BSP may serve as a nucleator of hydroxyapatite crystal formation. Durapatite 32-46 integrin binding sialoprotein Homo sapiens 0-3 11125546-9 2000 In the female group BSP was inversely related to serum estradiol levels (r = -0.274, p = 0.002) as to BMD (DEXA) at the lumbar spine and femoral neck. Estradiol 55-64 integrin binding sialoprotein Homo sapiens 20-23 10742656-2 2000 SP-II fraction obtained by SP-Sephadex C-25 chromatography showed the most potent superoxide anion scavenging activity (SOSA), and it was further separated into a peptide using an octadecylsilano-high performance liquid chromatography. sephadex c 30-40 integrin binding sialoprotein Homo sapiens 0-5 10785511-3 2000 BSP contains an Arg-Gly-Asp (RGD) motif found in other adhesive molecules that interact with cellular integrins. Arginine 16-19 integrin binding sialoprotein Homo sapiens 0-3 10785511-3 2000 BSP contains an Arg-Gly-Asp (RGD) motif found in other adhesive molecules that interact with cellular integrins. Glycine 20-23 integrin binding sialoprotein Homo sapiens 0-3 10785511-3 2000 BSP contains an Arg-Gly-Asp (RGD) motif found in other adhesive molecules that interact with cellular integrins. Aspartic Acid 24-27 integrin binding sialoprotein Homo sapiens 0-3 10753913-7 2000 Transient activation of PKA by either PTH-(1-34) or short term cAMP analog treatment blocks the deposition of BSP in the extracellular matrix without a significant reduction in the total amount of BSP synthesized and secreted. Cyclic AMP 63-67 integrin binding sialoprotein Homo sapiens 110-113 10742656-2 2000 SP-II fraction obtained by SP-Sephadex C-25 chromatography showed the most potent superoxide anion scavenging activity (SOSA), and it was further separated into a peptide using an octadecylsilano-high performance liquid chromatography. Superoxides 82-98 integrin binding sialoprotein Homo sapiens 0-5 9699501-2 1998 BSP possesses an integrin-binding RGD (Arg-Gly-Asp) domain, which may promote interactions between HBC cells and bone extracellular matrix. Arginine 39-42 integrin binding sialoprotein Homo sapiens 0-3 10632320-6 1999 The cutoff for elevated serum BSP values was 24 ng/ml, ie., two SDs above the normal mean value. Sodium Dodecyl Sulfate 64-67 integrin binding sialoprotein Homo sapiens 30-33 10632320-17 1999 Breast cancer patients with elevated serum BSP levels may benefit from osteoprotective adjuvant therapy with bisphosphonates. Diphosphonates 109-124 integrin binding sialoprotein Homo sapiens 43-46 10203417-10 1999 Immunoprecipitation analysis of OPN and BSP in the cell/matrix layers and the culture media after [35S]-methionine labeling showed their deposition primarily in the mineralized nodules of the Dex group, and their release into the media in the IL-1 group. Dexamethasone 192-195 integrin binding sialoprotein Homo sapiens 40-43 10203417-11 1999 Immunogold labeling demonstrated the location of OPN and BSP in mineralized nodules of the Dex group, but no significant labeling occurred in the nodule-like structures from the IL-1 group. Dexamethasone 91-94 integrin binding sialoprotein Homo sapiens 57-60 10759428-9 1999 In combination, the hydroxyapatite-binding polyglutamic acid sequences and the RGD provide bi-functional entities through which BSP may mediate the targeting and attachment of normal and metastasizing cells to the bone surface. Durapatite 20-34 integrin binding sialoprotein Homo sapiens 128-131 10759428-9 1999 In combination, the hydroxyapatite-binding polyglutamic acid sequences and the RGD provide bi-functional entities through which BSP may mediate the targeting and attachment of normal and metastasizing cells to the bone surface. Polyglutamic Acid 43-60 integrin binding sialoprotein Homo sapiens 128-131 9699501-2 1998 BSP possesses an integrin-binding RGD (Arg-Gly-Asp) domain, which may promote interactions between HBC cells and bone extracellular matrix. Glycine 43-46 integrin binding sialoprotein Homo sapiens 0-3 9699501-2 1998 BSP possesses an integrin-binding RGD (Arg-Gly-Asp) domain, which may promote interactions between HBC cells and bone extracellular matrix. Aspartic Acid 47-50 integrin binding sialoprotein Homo sapiens 0-3 9699501-4 1998 Recombinant BSP fragment analysis localized a significant component of these activities to the RGD domain of the protein, and synthetic RGD peptides with BSP flanking sequences (BSP-RGD) also conferred these responses. Peptides 140-148 integrin binding sialoprotein Homo sapiens 12-15 9699501-4 1998 Recombinant BSP fragment analysis localized a significant component of these activities to the RGD domain of the protein, and synthetic RGD peptides with BSP flanking sequences (BSP-RGD) also conferred these responses. Peptides 140-148 integrin binding sialoprotein Homo sapiens 154-157 9699501-4 1998 Recombinant BSP fragment analysis localized a significant component of these activities to the RGD domain of the protein, and synthetic RGD peptides with BSP flanking sequences (BSP-RGD) also conferred these responses. Peptides 140-148 integrin binding sialoprotein Homo sapiens 154-157 9648459-7 1998 BSP and osteopontin are sialoproteins containing a RGD cell-attachment sequence and poly(acidic amino acid) sequences. poly(acidic amino acid) 84-107 integrin binding sialoprotein Homo sapiens 0-3 9458353-13 1998 We speculate that the high expression of BSP could create an appropriate microenvironment for the crystallisation of calcium and phosphate into hydroxyapatite. Calcium 117-124 integrin binding sialoprotein Homo sapiens 41-44 9258751-3 1997 Both native BSP and a 47 kD fragment of UMR-BSP (Fragment 1 approximately 133A- approximately 265Y) are more potent inhibitors of seeded hydroxyapatite crystal growth than recombinant human BSP fragments lacking post-translational modifications. Durapatite 137-151 integrin binding sialoprotein Homo sapiens 12-15 9458353-13 1998 We speculate that the high expression of BSP could create an appropriate microenvironment for the crystallisation of calcium and phosphate into hydroxyapatite. Phosphates 129-138 integrin binding sialoprotein Homo sapiens 41-44 9458353-13 1998 We speculate that the high expression of BSP could create an appropriate microenvironment for the crystallisation of calcium and phosphate into hydroxyapatite. Durapatite 144-158 integrin binding sialoprotein Homo sapiens 41-44 9262507-4 1997 BSP contains the integrin binding peptide Arg-Gly-Asp (RGD), as well as non-RGD cell attachment domain. arginyl-glycyl-aspartic acid 42-53 integrin binding sialoprotein Homo sapiens 0-3 9258751-3 1997 Both native BSP and a 47 kD fragment of UMR-BSP (Fragment 1 approximately 133A- approximately 265Y) are more potent inhibitors of seeded hydroxyapatite crystal growth than recombinant human BSP fragments lacking post-translational modifications. Durapatite 137-151 integrin binding sialoprotein Homo sapiens 44-47 9258751-5 1997 BSP facilitates the adhesion of several cell types through its integrin binding (RGD) tripeptide sequence. tripeptide K-26 86-96 integrin binding sialoprotein Homo sapiens 0-3 9258751-8 1997 In this report, non-RGD cell adhesion sites are localized within conserved amino- and carboxy-terminal tyrosine-rich domains of recombinant human BSP. Tyrosine 103-111 integrin binding sialoprotein Homo sapiens 146-149 9181551-0 1997 Attachment of osteoblastic cells to hydroxyapatite crystals by a synthetic peptide (Glu7-Pro-Arg-Gly-Asp-Thr) containing two functional sequences of bone sialoprotein. Durapatite 36-50 integrin binding sialoprotein Homo sapiens 149-166 9181551-0 1997 Attachment of osteoblastic cells to hydroxyapatite crystals by a synthetic peptide (Glu7-Pro-Arg-Gly-Asp-Thr) containing two functional sequences of bone sialoprotein. Peptides 75-82 integrin binding sialoprotein Homo sapiens 149-166 9181551-1 1997 We investigated activity of bone sialoprotein (BSP) to mediate attachment of cells to hydroxyapatite using a model peptide, Glu7-Pro-Arg-Gly-Asp-Thr, which contains a putative hydroxyapatite-binding site (poly-Glu) and a cell-attachment site. Durapatite 86-100 integrin binding sialoprotein Homo sapiens 28-45 9181551-1 1997 We investigated activity of bone sialoprotein (BSP) to mediate attachment of cells to hydroxyapatite using a model peptide, Glu7-Pro-Arg-Gly-Asp-Thr, which contains a putative hydroxyapatite-binding site (poly-Glu) and a cell-attachment site. Durapatite 86-100 integrin binding sialoprotein Homo sapiens 47-50 9181551-1 1997 We investigated activity of bone sialoprotein (BSP) to mediate attachment of cells to hydroxyapatite using a model peptide, Glu7-Pro-Arg-Gly-Asp-Thr, which contains a putative hydroxyapatite-binding site (poly-Glu) and a cell-attachment site. Durapatite 176-190 integrin binding sialoprotein Homo sapiens 28-45 9181551-1 1997 We investigated activity of bone sialoprotein (BSP) to mediate attachment of cells to hydroxyapatite using a model peptide, Glu7-Pro-Arg-Gly-Asp-Thr, which contains a putative hydroxyapatite-binding site (poly-Glu) and a cell-attachment site. Durapatite 176-190 integrin binding sialoprotein Homo sapiens 47-50 9181551-1 1997 We investigated activity of bone sialoprotein (BSP) to mediate attachment of cells to hydroxyapatite using a model peptide, Glu7-Pro-Arg-Gly-Asp-Thr, which contains a putative hydroxyapatite-binding site (poly-Glu) and a cell-attachment site. poly-glu 205-213 integrin binding sialoprotein Homo sapiens 28-45 9181551-1 1997 We investigated activity of bone sialoprotein (BSP) to mediate attachment of cells to hydroxyapatite using a model peptide, Glu7-Pro-Arg-Gly-Asp-Thr, which contains a putative hydroxyapatite-binding site (poly-Glu) and a cell-attachment site. poly-glu 205-213 integrin binding sialoprotein Homo sapiens 47-50 9181551-9 1997 In conclusion, BSP mediated attachment of osteoblastic cells to hydroxyapatite, and this activity could be accomplished only by the poly-Glu sequence and the Arg-Gly-Asp sequence. Durapatite 64-78 integrin binding sialoprotein Homo sapiens 15-18 9181551-9 1997 In conclusion, BSP mediated attachment of osteoblastic cells to hydroxyapatite, and this activity could be accomplished only by the poly-Glu sequence and the Arg-Gly-Asp sequence. poly-glu 132-140 integrin binding sialoprotein Homo sapiens 15-18 9181551-9 1997 In conclusion, BSP mediated attachment of osteoblastic cells to hydroxyapatite, and this activity could be accomplished only by the poly-Glu sequence and the Arg-Gly-Asp sequence. Arginine 158-161 integrin binding sialoprotein Homo sapiens 15-18 9181551-9 1997 In conclusion, BSP mediated attachment of osteoblastic cells to hydroxyapatite, and this activity could be accomplished only by the poly-Glu sequence and the Arg-Gly-Asp sequence. Glycine 162-165 integrin binding sialoprotein Homo sapiens 15-18 9181551-9 1997 In conclusion, BSP mediated attachment of osteoblastic cells to hydroxyapatite, and this activity could be accomplished only by the poly-Glu sequence and the Arg-Gly-Asp sequence. Aspartic Acid 166-169 integrin binding sialoprotein Homo sapiens 15-18 8784085-13 1996 In a subgroup of 15 patients with metastatic BC, iv bisphosphonate treatment resulted in a rapid reduction of serum BSP levels to 40% of the baseline values within 4 days of treatment. Diphosphonates 52-66 integrin binding sialoprotein Homo sapiens 116-119 8939923-5 1996 Co-transfection of an expression plasmid encoding heat-stable inhibitor of cAMP-dependent protein kinase, a peptide inhibitor of PKA, decreased both the basal and PTH-induced bsp transcription, while co-expression of the catalytic subunit of PKA-induced bsp expression 3-fold. Cyclic AMP 75-79 integrin binding sialoprotein Homo sapiens 175-178 8939923-5 1996 Co-transfection of an expression plasmid encoding heat-stable inhibitor of cAMP-dependent protein kinase, a peptide inhibitor of PKA, decreased both the basal and PTH-induced bsp transcription, while co-expression of the catalytic subunit of PKA-induced bsp expression 3-fold. Cyclic AMP 75-79 integrin binding sialoprotein Homo sapiens 254-257 8939923-8 1996 A half-cAMP response element site in the bsp promoter was identified as the cis-acting element that mediated the PTH response by the transient transfections with reporter constructs containing nested deletions of the promoter or a heterologous promoter containing the cAMP response element. Cyclic AMP 7-11 integrin binding sialoprotein Homo sapiens 41-44 8939923-8 1996 A half-cAMP response element site in the bsp promoter was identified as the cis-acting element that mediated the PTH response by the transient transfections with reporter constructs containing nested deletions of the promoter or a heterologous promoter containing the cAMP response element. Cyclic AMP 268-272 integrin binding sialoprotein Homo sapiens 41-44 8939923-9 1996 In conclusion, these data indicate that PTH stimulation of bsp gene expression is specific to osteoblasts and mediated by changing cellular cAMP/PKA levels. Cyclic AMP 140-144 integrin binding sialoprotein Homo sapiens 59-62 9084661-4 1996 Our in situ phosphorylation experiments done with [gamma-32P] GTP suggest the existence of extracellular phosphorylation by a casein kinase II (CKII)-like enzyme, the enzyme known to phosphorylate most of the phosphate residues in dentin phosphophoryn and bone sialoproteins (osteopontin and BSP II). [gamma-32p] gtp 50-65 integrin binding sialoprotein Homo sapiens 292-298 8797881-1 1996 We have recently demonstrated that bone sialoprotein (BSP), a bone-matrix protein involved in hydroxyapatite crystal formation, is ectopically expressed in human breast cancers. Durapatite 94-108 integrin binding sialoprotein Homo sapiens 35-52 8797881-1 1996 We have recently demonstrated that bone sialoprotein (BSP), a bone-matrix protein involved in hydroxyapatite crystal formation, is ectopically expressed in human breast cancers. Durapatite 94-108 integrin binding sialoprotein Homo sapiens 54-57 8797881-3 1996 We analyzed BSP expression in 454 breast-cancer patients by immunohistochemistry on archival paraffin-embedded material using an anti-BSP polyclonal antibody. Paraffin 93-101 integrin binding sialoprotein Homo sapiens 12-15 8641185-1 1996 Bone sialoprotein (BSP) is a protein highly specific for bone, which contains an arginine-glycine-aspartic acid (RGD) cell attachment sequence involved in osteoclast adhesion to bone matrix via the vitronectin receptor. arginyl-glycyl-aspartic acid 81-111 integrin binding sialoprotein Homo sapiens 0-17 8641185-1 1996 Bone sialoprotein (BSP) is a protein highly specific for bone, which contains an arginine-glycine-aspartic acid (RGD) cell attachment sequence involved in osteoclast adhesion to bone matrix via the vitronectin receptor. arginyl-glycyl-aspartic acid 81-111 integrin binding sialoprotein Homo sapiens 19-22 9173083-5 1996 The observed increase in the alkaline phosphatase mRNA was effective at a Dex concentration as low as 10(-10) M with maximum values achieved at 10(-8)M. In contrast, Dex decreased the steady-state mRNA levels of both bone sialoprotein (BSP) and osteopontin (OPN) over a 4 week observation period when compared to the corresponding control values. Dexamethasone 166-169 integrin binding sialoprotein Homo sapiens 217-234 9173083-5 1996 The observed increase in the alkaline phosphatase mRNA was effective at a Dex concentration as low as 10(-10) M with maximum values achieved at 10(-8)M. In contrast, Dex decreased the steady-state mRNA levels of both bone sialoprotein (BSP) and osteopontin (OPN) over a 4 week observation period when compared to the corresponding control values. Dexamethasone 166-169 integrin binding sialoprotein Homo sapiens 236-239 9173083-6 1996 The relative BSP and OPN mRNA levels among the Dex treated cultures, however, showed a steady increase after more than 1 week exposure. Dexamethasone 47-50 integrin binding sialoprotein Homo sapiens 13-16 9173083-10 1996 With the induced increment in alkaline phosphatase correlating with the mineralization effects of Dex, the observed concomitant decrease in osteopontin and bone sialoprotein mRNA levels and the associated decline of osteocalcin are consistent with the hypothesis that the regulation of the expression of these highly negatively charged proteins is essential in order to maximize the Dex-induced mineralization process conditioned by normal human bone marrow stromal osteoprogenitor cells. Dexamethasone 98-101 integrin binding sialoprotein Homo sapiens 156-173 9084640-6 1996 The TATA box is overlapped by a vitamin D3 response element (VDRE) which appears to mediate vitamin D suppression of BSP gene transcription by competing with the TATA-binding protein (TBP) for occupancy of the site of the pre-initiation complex formation. Vitamin D 32-41 integrin binding sialoprotein Homo sapiens 117-120 9084679-1 1996 Bone sialoprotein (BSP) was shown to be a potent nucleator of hydroxyapatite (HA) in a steady-state agarose gel system (Hunter and Goldberg, 1993, PNAS 90: 8562). Durapatite 62-76 integrin binding sialoprotein Homo sapiens 0-23 9084679-1 1996 Bone sialoprotein (BSP) was shown to be a potent nucleator of hydroxyapatite (HA) in a steady-state agarose gel system (Hunter and Goldberg, 1993, PNAS 90: 8562). Sepharose 100-107 integrin binding sialoprotein Homo sapiens 0-23 9084679-3 1996 Since BSP contains contiguous sequences of glutamic acid, it is reasonable to suggest that the HA-nucleating activity of BSP resides within these regions. Glutamic Acid 43-56 integrin binding sialoprotein Homo sapiens 6-9 9084679-3 1996 Since BSP contains contiguous sequences of glutamic acid, it is reasonable to suggest that the HA-nucleating activity of BSP resides within these regions. Glutamic Acid 43-56 integrin binding sialoprotein Homo sapiens 121-124 9084679-6 1996 Each of these peptides contained one of the two glutamic acid-rich regions of porcine BSP. Glutamic Acid 48-61 integrin binding sialoprotein Homo sapiens 86-89 9084679-7 1996 In the steady-state agarose gel system, BSP, P1 and P2 induced HA formation, whereas the pooled small BSP-derived peptides (P3-5) did not. Sepharose 20-27 integrin binding sialoprotein Homo sapiens 40-43 9084679-9 1996 These findings suggest that the nucleating activity does not require intact molecules, that the nucleation of HA and BSP appears to require glutamic acid-rich sequences in a helical conformation and that there are two domains in porcine BSP that are each capable of nucleating HA. Glutamic Acid 140-153 integrin binding sialoprotein Homo sapiens 117-120 9084640-2 1996 Recent studies on the developmental expression of BSP mRNA and the temporo-spatial appearance of the protein during bone formation in vivo and in vitro have demonstrated that BSP is expressed by differentiated osteoblasts and that it may function in the initial nucleation of hydroxyapatite crystals in de novo bone formation. Durapatite 276-290 integrin binding sialoprotein Homo sapiens 175-178 9084640-6 1996 The TATA box is overlapped by a vitamin D3 response element (VDRE) which appears to mediate vitamin D suppression of BSP gene transcription by competing with the TATA-binding protein (TBP) for occupancy of the site of the pre-initiation complex formation. Cholecalciferol 32-42 integrin binding sialoprotein Homo sapiens 117-120 9084640-8 1996 Further upstream an AP-1 site, overlapped by a steroid hormone response-like sequence, mediates down-regulation of BSP transcription induced by TPA that is abrogated by a complex interaction between Jun and the glucocorticoid receptor protein induced by dexamethasone. Steroids 47-62 integrin binding sialoprotein Homo sapiens 115-118 9084640-8 1996 Further upstream an AP-1 site, overlapped by a steroid hormone response-like sequence, mediates down-regulation of BSP transcription induced by TPA that is abrogated by a complex interaction between Jun and the glucocorticoid receptor protein induced by dexamethasone. Tetradecanoylphorbol Acetate 144-147 integrin binding sialoprotein Homo sapiens 115-118 9084640-8 1996 Further upstream an AP-1 site, overlapped by a steroid hormone response-like sequence, mediates down-regulation of BSP transcription induced by TPA that is abrogated by a complex interaction between Jun and the glucocorticoid receptor protein induced by dexamethasone. Dexamethasone 254-267 integrin binding sialoprotein Homo sapiens 115-118 7915111-1 1994 Bone sialoprotein (BSP) is a bone-specific glycoprotein containing phosphoserine and sulphotyrosine residues and regions of contiguous glutamic acid residues. Phosphoserine 67-80 integrin binding sialoprotein Homo sapiens 0-17 7554919-0 1995 Regulation of bone sialoprotein gene transcription by steroid hormones. Steroids 54-70 integrin binding sialoprotein Homo sapiens 14-31 7554919-1 1995 During the initial formation of bone, dentine and cementum in tooth morphogenesis, fully differentiated osteoblasts, odontoblasts and cementoblasts express bone sialoprotein (BSP), a mineralized tissue-specific acidic glycoprotein that has been implicated in the nucleation of hydroxyapatite crystal growth. Durapatite 277-291 integrin binding sialoprotein Homo sapiens 156-173 7554919-1 1995 During the initial formation of bone, dentine and cementum in tooth morphogenesis, fully differentiated osteoblasts, odontoblasts and cementoblasts express bone sialoprotein (BSP), a mineralized tissue-specific acidic glycoprotein that has been implicated in the nucleation of hydroxyapatite crystal growth. Durapatite 277-291 integrin binding sialoprotein Homo sapiens 175-178 7554919-2 1995 The expression of BSP is regulated by steroid hormones that modulate mineralized tissue formation. Steroids 38-54 integrin binding sialoprotein Homo sapiens 18-21 7554919-4 1995 In contrast, however, vitamin D3 suppresses bone formation and abrogates the expression of BSP. Cholecalciferol 22-32 integrin binding sialoprotein Homo sapiens 91-94 7554919-5 1995 Our studies, using the osteoblastic cell lines ROS 17/2.8 and UMR 106-06, have revealed that the glucocorticoid (10(-8) M dexamethasone; dex) effect on BSP mRNA involves both direct and indirect pathways. ros 47-50 integrin binding sialoprotein Homo sapiens 152-155 7554919-5 1995 Our studies, using the osteoblastic cell lines ROS 17/2.8 and UMR 106-06, have revealed that the glucocorticoid (10(-8) M dexamethasone; dex) effect on BSP mRNA involves both direct and indirect pathways. Dexamethasone 122-135 integrin binding sialoprotein Homo sapiens 152-155 7554919-5 1995 Our studies, using the osteoblastic cell lines ROS 17/2.8 and UMR 106-06, have revealed that the glucocorticoid (10(-8) M dexamethasone; dex) effect on BSP mRNA involves both direct and indirect pathways. Dextromethorphan 122-125 integrin binding sialoprotein Homo sapiens 152-155 7554919-7 1995 The promoters are characterized by a highly conserved region (BSP box) encompassing the immediate promoter region, which includes a unique inverted TATA box overlapped by a putative (DR3) vitamin D3 response element (VDRE). Cholecalciferol 188-198 integrin binding sialoprotein Homo sapiens 62-65 9084679-9 1996 These findings suggest that the nucleating activity does not require intact molecules, that the nucleation of HA and BSP appears to require glutamic acid-rich sequences in a helical conformation and that there are two domains in porcine BSP that are each capable of nucleating HA. Glutamic Acid 140-153 integrin binding sialoprotein Homo sapiens 237-240 9084680-1 1996 Bone sialoprotein (BSP) and osteopontin (OPN) are two extracellular bone matrix proteins that have the ability to modulate the growth of hydroxyapatite in vitro. Durapatite 137-151 integrin binding sialoprotein Homo sapiens 0-23 9084680-2 1996 Studies of BSP/OPN hydroxyapatite interactions in the past have been directed toward the identification of essential structural elements that allow these two proteins to modulate hydroxyapatite growth. Durapatite 19-33 integrin binding sialoprotein Homo sapiens 11-14 9084680-2 1996 Studies of BSP/OPN hydroxyapatite interactions in the past have been directed toward the identification of essential structural elements that allow these two proteins to modulate hydroxyapatite growth. Durapatite 179-193 integrin binding sialoprotein Homo sapiens 11-14 7754798-1 1995 Biosynthesis of bone sialoprotein (BSP) by a human osteoclastic cell line (FLG 29.1) during its differentiation induced by phorbol 12-myristate 13-acetate (TPA) was studied using metabolic radiolabeling experiments. Tetradecanoylphorbol Acetate 123-154 integrin binding sialoprotein Homo sapiens 16-33 7754798-1 1995 Biosynthesis of bone sialoprotein (BSP) by a human osteoclastic cell line (FLG 29.1) during its differentiation induced by phorbol 12-myristate 13-acetate (TPA) was studied using metabolic radiolabeling experiments. Tetradecanoylphorbol Acetate 123-154 integrin binding sialoprotein Homo sapiens 35-38 7754798-1 1995 Biosynthesis of bone sialoprotein (BSP) by a human osteoclastic cell line (FLG 29.1) during its differentiation induced by phorbol 12-myristate 13-acetate (TPA) was studied using metabolic radiolabeling experiments. Tetradecanoylphorbol Acetate 156-159 integrin binding sialoprotein Homo sapiens 16-33 7754798-1 1995 Biosynthesis of bone sialoprotein (BSP) by a human osteoclastic cell line (FLG 29.1) during its differentiation induced by phorbol 12-myristate 13-acetate (TPA) was studied using metabolic radiolabeling experiments. Tetradecanoylphorbol Acetate 156-159 integrin binding sialoprotein Homo sapiens 35-38 7754798-3 1995 One of the major glycoproteins synthesized by the TPA-treated FLG 29.1 cells was sulfated, had an identical electrophoretic mobility to purified BSP, and could be immunoprecipitated with a specific antibody against human BSP (LF 6). Tetradecanoylphorbol Acetate 50-53 integrin binding sialoprotein Homo sapiens 145-148 7754798-3 1995 One of the major glycoproteins synthesized by the TPA-treated FLG 29.1 cells was sulfated, had an identical electrophoretic mobility to purified BSP, and could be immunoprecipitated with a specific antibody against human BSP (LF 6). Tetradecanoylphorbol Acetate 50-53 integrin binding sialoprotein Homo sapiens 221-224 7754798-5 1995 Furthermore, mRNA for BSP was also detected in TPA-treated FLG 29.1 cells by RNA-polymerase chain reaction. Tetradecanoylphorbol Acetate 47-50 integrin binding sialoprotein Homo sapiens 22-25 7754798-7 1995 Immunocytochemistry using an anti-BSP antibody showed a prominent paranuclear (suggestive of Golgi apparatus) localization of BSP in the TPA-treated FLG 29.1 cells after permeabilization, while untreated cells were not significantly immunostained. Tetradecanoylphorbol Acetate 137-140 integrin binding sialoprotein Homo sapiens 34-37 7754798-7 1995 Immunocytochemistry using an anti-BSP antibody showed a prominent paranuclear (suggestive of Golgi apparatus) localization of BSP in the TPA-treated FLG 29.1 cells after permeabilization, while untreated cells were not significantly immunostained. Tetradecanoylphorbol Acetate 137-140 integrin binding sialoprotein Homo sapiens 126-129 7754798-8 1995 Localization of BSP at the plasma membrane was also demonstrated in the TPA-treated FLG 29.1 cells by the fluorescence-activated cell sorting analysis. Tetradecanoylphorbol Acetate 72-75 integrin binding sialoprotein Homo sapiens 16-19 7754798-9 1995 Since TPA has been demonstrated to induce expression of various osteoclastic characteristics in FLG 29.1 cells, induction of BSP expression by TPA suggests that the protein may play a role during the differentiation process of osteoclasts or in functions of differentiated osteoclasts. Tetradecanoylphorbol Acetate 143-146 integrin binding sialoprotein Homo sapiens 125-128 7915111-1 1994 Bone sialoprotein (BSP) is a bone-specific glycoprotein containing phosphoserine and sulphotyrosine residues and regions of contiguous glutamic acid residues. Phosphoserine 67-80 integrin binding sialoprotein Homo sapiens 19-22 7915111-1 1994 Bone sialoprotein (BSP) is a bone-specific glycoprotein containing phosphoserine and sulphotyrosine residues and regions of contiguous glutamic acid residues. sulphotyrosine 85-99 integrin binding sialoprotein Homo sapiens 0-17 7915111-1 1994 Bone sialoprotein (BSP) is a bone-specific glycoprotein containing phosphoserine and sulphotyrosine residues and regions of contiguous glutamic acid residues. sulphotyrosine 85-99 integrin binding sialoprotein Homo sapiens 19-22 7915111-1 1994 Bone sialoprotein (BSP) is a bone-specific glycoprotein containing phosphoserine and sulphotyrosine residues and regions of contiguous glutamic acid residues. Glutamic Acid 135-148 integrin binding sialoprotein Homo sapiens 0-17 7915111-1 1994 Bone sialoprotein (BSP) is a bone-specific glycoprotein containing phosphoserine and sulphotyrosine residues and regions of contiguous glutamic acid residues. Glutamic Acid 135-148 integrin binding sialoprotein Homo sapiens 19-22 7915111-2 1994 Recent studies in this laboratory have shown that BSP is capable of nucleating the bone mineral hydroxyapatite in a steady-state agarose gel system. Durapatite 96-110 integrin binding sialoprotein Homo sapiens 50-53 7915111-2 1994 Recent studies in this laboratory have shown that BSP is capable of nucleating the bone mineral hydroxyapatite in a steady-state agarose gel system. Sepharose 129-136 integrin binding sialoprotein Homo sapiens 50-53 7915111-3 1994 We show here that chemical modification of carboxylate groups abolishes the nucleation activity of BSP, but enzymic dephosphorylation has no effect. carboxylate 43-54 integrin binding sialoprotein Homo sapiens 99-102 7915111-6 1994 These findings suggest that the nucleation of hydroxyapatite by BSP involves one or both of the glutamic acid-rich sequences. Durapatite 46-60 integrin binding sialoprotein Homo sapiens 64-67 7915111-6 1994 These findings suggest that the nucleation of hydroxyapatite by BSP involves one or both of the glutamic acid-rich sequences. Glutamic Acid 96-109 integrin binding sialoprotein Homo sapiens 64-67 8169152-1 1994 Bone sialoprotein (BSP) and osteopontin (OPN) are two major non-collagenous proteins in bone that have similar biochemical properties and can mediate cell attachment through an RGD (Arg-Gly-Asp) motif that recognizes the vitronectin receptor. arginyl-glycyl-aspartic acid 182-193 integrin binding sialoprotein Homo sapiens 0-23 8106456-12 1994 Using normal human bone cells, the cell attachment activity of the reduced sulfate form of BSP was nearly equivalent to that of the fully sulfated product. Sulfates 75-82 integrin binding sialoprotein Homo sapiens 91-94 8187059-3 1994 Bone sialoprotein (BSP) is a glycoprotein the expression of which coincides with the appearance of the first hydroxyapatite crystals during bone development. Durapatite 109-123 integrin binding sialoprotein Homo sapiens 0-17 8187059-3 1994 Bone sialoprotein (BSP) is a glycoprotein the expression of which coincides with the appearance of the first hydroxyapatite crystals during bone development. Durapatite 109-123 integrin binding sialoprotein Homo sapiens 19-22 8187059-6 1994 Two polyclonal antibodies, one directed against intact human BSP and the other against a synthetic peptide of BSP (residues 277-294), were used and gave identical results. Peptides 99-106 integrin binding sialoprotein Homo sapiens 110-113 8318316-1 1993 Bone sialoprotein contains a cell-binding RGD sequence followed by a threonine residue. Threonine 69-78 integrin binding sialoprotein Homo sapiens 0-17 8106009-10 1993 The data indicate win4 and BSP genes are differentially regulated, and their products may play important roles in the storage and reallocation of nitrogen in perennial plants. Nitrogen 146-154 integrin binding sialoprotein Homo sapiens 27-30 8397409-4 1993 Gels containing BSP at 1-5 micrograms/ml, however, exhibited a visible precipitation band and significantly elevated Ca + PO4 contents. ca + po4 117-125 integrin binding sialoprotein Homo sapiens 16-19 8397409-5 1993 By powder x-ray diffraction, the precipitate formed in the presence of BSP was shown to be hydroxyapatite. Durapatite 91-105 integrin binding sialoprotein Homo sapiens 71-74 8318316-5 1993 The phospho-peptide inhibited binding of R1 cells to BSP-coated surfaces 10 times less efficiently compared with the non-phosphorylated peptide, as did, surprisingly, also the fibronectin-derived peptide Gly-Arg-Gly-Asp-Ser-Pro. Glycine 204-207 integrin binding sialoprotein Homo sapiens 53-56 8318316-5 1993 The phospho-peptide inhibited binding of R1 cells to BSP-coated surfaces 10 times less efficiently compared with the non-phosphorylated peptide, as did, surprisingly, also the fibronectin-derived peptide Gly-Arg-Gly-Asp-Ser-Pro. Serine 220-223 integrin binding sialoprotein Homo sapiens 53-56 8489418-2 1993 Although BSP can mediate cell attachment through an RGD sequence and binds selectively to hydroxyapatite, its precise function in mineralized tissues is unknown. Durapatite 90-104 integrin binding sialoprotein Homo sapiens 9-12 8419458-0 1993 Bone sialoprotein (BSP) secretion and osteoblast differentiation: relationship to bromodeoxyuridine incorporation, alkaline phosphatase, and matrix deposition. Bromodeoxyuridine 82-99 integrin binding sialoprotein Homo sapiens 0-17 8419458-0 1993 Bone sialoprotein (BSP) secretion and osteoblast differentiation: relationship to bromodeoxyuridine incorporation, alkaline phosphatase, and matrix deposition. Bromodeoxyuridine 82-99 integrin binding sialoprotein Homo sapiens 19-22