PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 31751560-10 2020 Additionally, treatment with 3-deazaneplanocin A (DZNep, an inhibitor of EZH2) inhibited expression of EZH2, increased protein level of ARHI, and partially abrogated the promoting effects of ARHI knockdown on glioma cell proliferation and migration. 3-deazaneplanocin 29-48 DIRAS family GTPase 3 Homo sapiens 136-140 34189759-0 2022 Overexpression of ARHI increases the sensitivity of cervical cancer cells to paclitaxel through inducing apoptosis and autophagy. Paclitaxel 77-87 DIRAS family GTPase 3 Homo sapiens 18-22 34189759-8 2022 The results revealed that the levels of ARHI mRNA and protein were down-regulated in CC tissues, and were further reduced in paclitaxel-resistant tissues and Hela cell model. Paclitaxel 125-135 DIRAS family GTPase 3 Homo sapiens 40-44 34189759-12 2022 In conclusion, overexpression of ARHI can increase the sensitivity of CC to paclitaxel through promoting apoptosis and autophagy in a AKT/mTOR inactivation dependent pathway. Paclitaxel 76-86 DIRAS family GTPase 3 Homo sapiens 33-37 35565270-0 2022 Resveratrol Contrasts IL-6 Pro-Growth Effects and Promotes Autophagy-Mediated Cancer Cell Dormancy in 3D Ovarian Cancer: Role of miR-1305 and of Its Target ARH-I. Resveratrol 0-11 DIRAS family GTPase 3 Homo sapiens 156-161 35565270-5 2022 IL-6 disrupts autophagy in ovarian cancer cells via miRNAs downregulation of ARH-I, an effect contrasted by the nutraceutical protein restriction mimetic resveratrol (RV). Resveratrol 154-165 DIRAS family GTPase 3 Homo sapiens 77-82 35565270-5 2022 IL-6 disrupts autophagy in ovarian cancer cells via miRNAs downregulation of ARH-I, an effect contrasted by the nutraceutical protein restriction mimetic resveratrol (RV). Resveratrol 167-169 DIRAS family GTPase 3 Homo sapiens 77-82 2719939-10 1989 In addition, the specificity of ARH-I toward dinucleoside 3",5"-phosphates is qualitatively similar to RNase A; while angiogenin prefers cytidylyl(3"----5")guanosine (CpG) to UpA, both RNase and the hybrid prefer UpA to CpG. dinucleoside 3",5"-phosphates 45-74 DIRAS family GTPase 3 Homo sapiens 32-37 2719939-10 1989 In addition, the specificity of ARH-I toward dinucleoside 3",5"-phosphates is qualitatively similar to RNase A; while angiogenin prefers cytidylyl(3"----5")guanosine (CpG) to UpA, both RNase and the hybrid prefer UpA to CpG. Cyclic GMP 147-165 DIRAS family GTPase 3 Homo sapiens 32-37 32484926-8 2020 RESULTS: Induction of autophagy by reexpression of DIRAS3 or serum starvation in multiple OvCa cell lines significantly reduced the 50% inhibitory concentration of crizotinib and other ALK inhibitors. Crizotinib 164-174 DIRAS family GTPase 3 Homo sapiens 51-57 32484926-9 2020 In 2 human OvCa xenograft models, the DIRAS3-expressing tumors treated with crizotinib had significantly decreased tumor burden and long-term survival in 67% to 79% of mice. Crizotinib 76-86 DIRAS family GTPase 3 Homo sapiens 38-44 31751560-10 2020 Additionally, treatment with 3-deazaneplanocin A (DZNep, an inhibitor of EZH2) inhibited expression of EZH2, increased protein level of ARHI, and partially abrogated the promoting effects of ARHI knockdown on glioma cell proliferation and migration. 3-deazaneplanocin 29-48 DIRAS family GTPase 3 Homo sapiens 191-195 31751560-10 2020 Additionally, treatment with 3-deazaneplanocin A (DZNep, an inhibitor of EZH2) inhibited expression of EZH2, increased protein level of ARHI, and partially abrogated the promoting effects of ARHI knockdown on glioma cell proliferation and migration. 3-deazaneplanocin 50-55 DIRAS family GTPase 3 Homo sapiens 136-140 31751560-10 2020 Additionally, treatment with 3-deazaneplanocin A (DZNep, an inhibitor of EZH2) inhibited expression of EZH2, increased protein level of ARHI, and partially abrogated the promoting effects of ARHI knockdown on glioma cell proliferation and migration. 3-deazaneplanocin 50-55 DIRAS family GTPase 3 Homo sapiens 191-195 34462379-11 2021 These findings provide convincing evidence that NOEY2-N regulates endothelial cell function and angiogenesis by interrupting the VEGFR-2/PDK-1/GSK-3beta signal transduction and thus strongly suggest that NOEY2-N might serve as a novel anti-tumour and anti-angiogenic agent against many diseases, including ovarian cancer. noey2-n 204-211 DIRAS family GTPase 3 Homo sapiens 48-53 33038921-9 2020 MTT and flow cytometric analysis revealed that DIRAS3 and p53 re-expression significantly inhibited proliferation and induced cell cycle arrest (P < 0.001). monooxyethylene trimethylolpropane tristearate 0-3 DIRAS family GTPase 3 Homo sapiens 47-53 31077613-0 2019 Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer. Melatonin 87-96 DIRAS family GTPase 3 Homo sapiens 46-50 31825828-4 2019 Disruption of K-RAS cluster formation requires the N terminus of DIRAS3 and interaction of both DIRAS3 and K-RAS with the plasma membrane. Nitrogen 51-52 DIRAS family GTPase 3 Homo sapiens 65-71 31077613-3 2019 Signal transducer and activator of transcription 3 (STAT3), frequently overexpressed and activated in paclitaxel (PTX)-resistant breast cancer, promotes the expression of DNA methyltransferase one (DNMT1) to epigenetically suppress the transcription of tumor suppressor Aplasia Ras homolog one (ARHI) which can sequester STAT3 in the cytoplasm to block PTX resistance. Paclitaxel 353-356 DIRAS family GTPase 3 Homo sapiens 295-299 31077613-0 2019 Epigenetic inhibition of the tumor suppressor ARHI by light at night-induced circadian melatonin disruption mediates STAT3-driven paclitaxel resistance in breast cancer. Paclitaxel 130-140 DIRAS family GTPase 3 Homo sapiens 46-50 31077613-3 2019 Signal transducer and activator of transcription 3 (STAT3), frequently overexpressed and activated in paclitaxel (PTX)-resistant breast cancer, promotes the expression of DNA methyltransferase one (DNMT1) to epigenetically suppress the transcription of tumor suppressor Aplasia Ras homolog one (ARHI) which can sequester STAT3 in the cytoplasm to block PTX resistance. Paclitaxel 102-112 DIRAS family GTPase 3 Homo sapiens 295-299 31077613-3 2019 Signal transducer and activator of transcription 3 (STAT3), frequently overexpressed and activated in paclitaxel (PTX)-resistant breast cancer, promotes the expression of DNA methyltransferase one (DNMT1) to epigenetically suppress the transcription of tumor suppressor Aplasia Ras homolog one (ARHI) which can sequester STAT3 in the cytoplasm to block PTX resistance. Paclitaxel 114-117 DIRAS family GTPase 3 Homo sapiens 295-299 30266404-5 2018 Further mechanistic study showed that the expression level of DIRAS3 in Classical and Mesenchymal subtype GBMs is higher, and over-expression of DIRAS3 promotes EGFR-AKT signaling activation at the downstream of EGFR and increases AKT phosphorylation, meanwhile suppression of AKT by MK-2206 reverses the tumor promoting function of DIRAS3. MK 2206 284-291 DIRAS family GTPase 3 Homo sapiens 62-68 31088402-8 2019 Autophagy inhibitor chloroquine (CQ) was used to study the effects of inhibition at late stage of autophagy on ARHI-induced autophagy and apoptosis. Chloroquine 20-31 DIRAS family GTPase 3 Homo sapiens 111-115 31088402-8 2019 Autophagy inhibitor chloroquine (CQ) was used to study the effects of inhibition at late stage of autophagy on ARHI-induced autophagy and apoptosis. Chloroquine 33-35 DIRAS family GTPase 3 Homo sapiens 111-115 31088402-15 2019 And chloroquine (CQ) used as an auxiliary medicine in glioma chemotherapy can enhance the antitumor effect of ARHI, and this study provides a novel mechanistic basis and strategy for glioma therapy. Chloroquine 4-15 DIRAS family GTPase 3 Homo sapiens 110-114 31088402-15 2019 And chloroquine (CQ) used as an auxiliary medicine in glioma chemotherapy can enhance the antitumor effect of ARHI, and this study provides a novel mechanistic basis and strategy for glioma therapy. Chloroquine 17-19 DIRAS family GTPase 3 Homo sapiens 110-114 30266404-5 2018 Further mechanistic study showed that the expression level of DIRAS3 in Classical and Mesenchymal subtype GBMs is higher, and over-expression of DIRAS3 promotes EGFR-AKT signaling activation at the downstream of EGFR and increases AKT phosphorylation, meanwhile suppression of AKT by MK-2206 reverses the tumor promoting function of DIRAS3. MK 2206 284-291 DIRAS family GTPase 3 Homo sapiens 145-151 30266404-5 2018 Further mechanistic study showed that the expression level of DIRAS3 in Classical and Mesenchymal subtype GBMs is higher, and over-expression of DIRAS3 promotes EGFR-AKT signaling activation at the downstream of EGFR and increases AKT phosphorylation, meanwhile suppression of AKT by MK-2206 reverses the tumor promoting function of DIRAS3. MK 2206 284-291 DIRAS family GTPase 3 Homo sapiens 145-151 28316325-2 2017 In the present study, we analyse the impact of the small GTPase, GTP-binding RAS-like 3 (DIRAS3), a recently identified weight-loss target gene, on cellular senescence in adipose stromal/progenitor cells (ASCs) derived from human subcutaneous white adipose tissue (sWAT). Guanosine Triphosphate 57-60 DIRAS family GTPase 3 Homo sapiens 89-95 29504523-10 2018 Resveratrol may inactivate STAT3 signaling of ovarian cancer cells in ARHI unrelated pattern(s). Resveratrol 0-11 DIRAS family GTPase 3 Homo sapiens 70-74 29201236-11 2017 Lithium chloride, a wnt/beta-catenin signaling activator, was able to attenuate the effect of ARHI on HCT116 cell invasion and adhesion. Lithium Chloride 0-16 DIRAS family GTPase 3 Homo sapiens 94-98 29504523-0 2018 Correlation of ARHI upregulation with growth suppression and STAT3 inactivation in resveratrol-treated ovarian cancer cells. Resveratrol 83-94 DIRAS family GTPase 3 Homo sapiens 15-19 29504523-3 2018 OBJECTIVE: The current study aims to elucidate the status of ARHI expression and its relevance with growth suppression and STAT3 inactivation of resveratrol-treated cells. Resveratrol 145-156 DIRAS family GTPase 3 Homo sapiens 61-65 29504523-5 2018 The involvement of ARHI in the growth inhibition and STAT3 inactivation of resveratrol-treated OVCAR-3 cells was investigated by transfection of ARHI-specific siRNA. Resveratrol 75-86 DIRAS family GTPase 3 Homo sapiens 19-23 29504523-5 2018 The involvement of ARHI in the growth inhibition and STAT3 inactivation of resveratrol-treated OVCAR-3 cells was investigated by transfection of ARHI-specific siRNA. Resveratrol 75-86 DIRAS family GTPase 3 Homo sapiens 145-149 29504523-6 2018 RESULTS: ARHI is expressed in low levels in three ovarian cancer cell lines, which is upregulated upon resveratrol treatment accompanied with growth arrest, extensive apoptosis, increased autophagic activity and inactivated STAT3 signaling. Resveratrol 103-114 DIRAS family GTPase 3 Homo sapiens 9-13 29504523-7 2018 Specific siRNA transfection efficiently knocked down ARHI expression in resveratrol-treated CAOV-3 and OVCAR-3 cells and increased the total cell number in limited extents (P> 0.05) in comparison with that of resveratrol-treated ovarian cancer cells without any transfection or transfected with mock oligonucleotides. Resveratrol 72-83 DIRAS family GTPase 3 Homo sapiens 53-57 29504523-7 2018 Specific siRNA transfection efficiently knocked down ARHI expression in resveratrol-treated CAOV-3 and OVCAR-3 cells and increased the total cell number in limited extents (P> 0.05) in comparison with that of resveratrol-treated ovarian cancer cells without any transfection or transfected with mock oligonucleotides. Resveratrol 212-223 DIRAS family GTPase 3 Homo sapiens 53-57 29504523-7 2018 Specific siRNA transfection efficiently knocked down ARHI expression in resveratrol-treated CAOV-3 and OVCAR-3 cells and increased the total cell number in limited extents (P> 0.05) in comparison with that of resveratrol-treated ovarian cancer cells without any transfection or transfected with mock oligonucleotides. Oligonucleotides 303-319 DIRAS family GTPase 3 Homo sapiens 53-57 29504523-9 2018 CONCLUSION: ARHI upregulation is another molecular event caused by resveratrol and one of the elements related with resveratrol"s anti-ovarian cancer efficacy. Resveratrol 67-78 DIRAS family GTPase 3 Homo sapiens 12-16 29504523-9 2018 CONCLUSION: ARHI upregulation is another molecular event caused by resveratrol and one of the elements related with resveratrol"s anti-ovarian cancer efficacy. Resveratrol 116-127 DIRAS family GTPase 3 Homo sapiens 12-16 28860610-6 2017 Longer LTL was associated with the reduced incidence of ARHI (adjusted OR = 0.550, 95% CI: 0.420-0.721, P < 0.0001 for all the ARHI; 0.498, 0.318-0.780, P = 0.0023 for FL subgroup; 0.428, 0.292-0.628, P < 0.0001 for AL subgroup; 0.552, 0.399-0.764, P = 0.0003 for mSL subgroup). Aluminum 222-224 DIRAS family GTPase 3 Homo sapiens 56-60 28316325-7 2017 DIRAS3 KD ASCs form senescence-associated heterochromatic foci as shown by increased level of gamma-H2A.X positive foci. gamma-h2a 94-103 DIRAS family GTPase 3 Homo sapiens 0-6 29192415-1 2017 OBJECTIVES: Downregulation of DIRAS3 (DIRAS family, GTP-binding Ras-like 3) is related to ovarian and breast cancer progression. Guanosine Triphosphate 52-55 DIRAS family GTPase 3 Homo sapiens 30-36 27787915-4 2017 Among the several potential candidates responsible for the anti-invasive effect promoted by Resveratrol, here we focused our attention on ARH-I (DIRAS3), that encodes a Ras homolog GTPase of 26-kDa. Resveratrol 92-103 DIRAS family GTPase 3 Homo sapiens 138-143 27787915-4 2017 Among the several potential candidates responsible for the anti-invasive effect promoted by Resveratrol, here we focused our attention on ARH-I (DIRAS3), that encodes a Ras homolog GTPase of 26-kDa. Resveratrol 92-103 DIRAS family GTPase 3 Homo sapiens 145-151 27787915-7 2017 On opposite, Resveratrol could counteract the IL-6 induction of cell migration in ovarian cancer cells through induction of autophagy in the cells at the migration front, which was paralleled by up-regulation of ARH-I and down-regulation of STAT3 expression. Resveratrol 13-24 DIRAS family GTPase 3 Homo sapiens 212-217 29192415-12 2017 Relative balance between methylated and unmethylated variants of DIRAS3 after MS-PCR was shifted towards unmethylated version after DNMTi treatment in A2780 cells. dnmti 132-137 DIRAS family GTPase 3 Homo sapiens 65-71 29192415-13 2017 Statistically significant dose dependent effect of decitabine and RG108 on DIRAS3 expression in A2780 cells was observed. Decitabine 51-61 DIRAS family GTPase 3 Homo sapiens 75-81 27685841-0 2016 Zebularine enhances apoptosis of human osteosarcoma cells by suppressing methylation of ARHI. pyrimidin-2-one beta-ribofuranoside 0-10 DIRAS family GTPase 3 Homo sapiens 88-92 27685841-3 2016 Our study aims to investigate the effect and mechanism of action of zebularine on the epigenetic modification of the ARHI gene, and whether this effect may modulate the viability and apoptosis of human osteosarcoma cells. pyrimidin-2-one beta-ribofuranoside 68-78 DIRAS family GTPase 3 Homo sapiens 117-121 27685841-5 2016 Zebularine potentiated the expression of ARHI at both the protein and mRNA level. pyrimidin-2-one beta-ribofuranoside 0-10 DIRAS family GTPase 3 Homo sapiens 41-45 27685841-6 2016 This was related to the downregulation of methylation of ARHI caused by zebularine. pyrimidin-2-one beta-ribofuranoside 72-82 DIRAS family GTPase 3 Homo sapiens 57-61 27685841-10 2016 Moreover, knockdown of ARHI rescued cell viability and apoptosis under the zebularine-treated condition. pyrimidin-2-one beta-ribofuranoside 75-85 DIRAS family GTPase 3 Homo sapiens 23-27 27685841-11 2016 We showed that zebularine inhibited viability and promoted apoptosis by disturbing the interaction between DNMT1 and G9a, thereby resulting in lower ARHI methylation and elevated ARHI expression in osteosarcoma cells. pyrimidin-2-one beta-ribofuranoside 15-25 DIRAS family GTPase 3 Homo sapiens 149-153 27685841-11 2016 We showed that zebularine inhibited viability and promoted apoptosis by disturbing the interaction between DNMT1 and G9a, thereby resulting in lower ARHI methylation and elevated ARHI expression in osteosarcoma cells. pyrimidin-2-one beta-ribofuranoside 15-25 DIRAS family GTPase 3 Homo sapiens 179-183 25193278-11 2014 Overexpression of Sp1 inhibited breast cancer cell migratory and invasive abilities, whereas knockdown of GTP-binding RAS-like 3 (DIRAS3, also known as ARHI, NOEY2) attenuated the inhibitory effects. Guanosine Triphosphate 106-109 DIRAS family GTPase 3 Homo sapiens 130-136 27368419-9 2016 This mechanism, which is shared between A-RAF and C-RAF, may be involved in the regulation of Ras12V-induced cell transformation by DiRas3. ras12v 94-100 DIRAS family GTPase 3 Homo sapiens 132-138 26247722-0 2015 ARHI (DIRAS3)-mediated autophagy-associated cell death enhances chemosensitivity to cisplatin in ovarian cancer cell lines and xenografts. Cisplatin 84-93 DIRAS family GTPase 3 Homo sapiens 0-4 26247722-0 2015 ARHI (DIRAS3)-mediated autophagy-associated cell death enhances chemosensitivity to cisplatin in ovarian cancer cell lines and xenografts. Cisplatin 84-93 DIRAS family GTPase 3 Homo sapiens 6-12 26247722-7 2015 Re-expression of ARHI enhanced the cytotoxic effect of cisplatin in cell culture, increasing caspase-3 activation and PARP cleavage by inhibiting ERK and HER2 activity and downregulating XIAP and Bcl-2. Cisplatin 55-64 DIRAS family GTPase 3 Homo sapiens 17-21 26247722-8 2015 In xenografts, treatment with cisplatin significantly slowed the outgrowth of dormant autophagic cells after reduction of ARHI, but the addition of chloroquine did not further inhibit xenograft outgrowth. Cisplatin 30-39 DIRAS family GTPase 3 Homo sapiens 122-126 25499977-5 2015 The transition of ARHI is marked by the movement of switch I region towards gamma-phosphate of GTP, in addition, the hydrophobic interaction between N-terminal helix and switch II region of ARHI accounts for its low intrinsic GTPase activity. gamma-phosphate 76-91 DIRAS family GTPase 3 Homo sapiens 18-22 25499977-5 2015 The transition of ARHI is marked by the movement of switch I region towards gamma-phosphate of GTP, in addition, the hydrophobic interaction between N-terminal helix and switch II region of ARHI accounts for its low intrinsic GTPase activity. Guanosine Triphosphate 95-98 DIRAS family GTPase 3 Homo sapiens 18-22 25077680-7 2015 (1) The expression of EZH2 inversely correlated with ARHI expression levels and predicted shorter overall survival in EOC patients; (2) EZH2 promoted repression of ARHI by catalyzing trimethylation on H3K27; (3) ARHI was synergistically silenced by DNA methylation and histone modification; and (4) DZNep, an inhibitor of EZH2, significantly reduced survival rate of EOC cells by restoring ARHI expression. 3-deazaneplanocin 299-304 DIRAS family GTPase 3 Homo sapiens 53-57 25077680-7 2015 (1) The expression of EZH2 inversely correlated with ARHI expression levels and predicted shorter overall survival in EOC patients; (2) EZH2 promoted repression of ARHI by catalyzing trimethylation on H3K27; (3) ARHI was synergistically silenced by DNA methylation and histone modification; and (4) DZNep, an inhibitor of EZH2, significantly reduced survival rate of EOC cells by restoring ARHI expression. 3-deazaneplanocin 299-304 DIRAS family GTPase 3 Homo sapiens 164-168 25077680-7 2015 (1) The expression of EZH2 inversely correlated with ARHI expression levels and predicted shorter overall survival in EOC patients; (2) EZH2 promoted repression of ARHI by catalyzing trimethylation on H3K27; (3) ARHI was synergistically silenced by DNA methylation and histone modification; and (4) DZNep, an inhibitor of EZH2, significantly reduced survival rate of EOC cells by restoring ARHI expression. 3-deazaneplanocin 299-304 DIRAS family GTPase 3 Homo sapiens 164-168 25077680-7 2015 (1) The expression of EZH2 inversely correlated with ARHI expression levels and predicted shorter overall survival in EOC patients; (2) EZH2 promoted repression of ARHI by catalyzing trimethylation on H3K27; (3) ARHI was synergistically silenced by DNA methylation and histone modification; and (4) DZNep, an inhibitor of EZH2, significantly reduced survival rate of EOC cells by restoring ARHI expression. 3-deazaneplanocin 299-304 DIRAS family GTPase 3 Homo sapiens 164-168 25077680-9 2015 Suppression of EZH2 by DZNep, as a way of restoring the expression of ARHI, could be a potential treatment modality to EOC. 3-deazaneplanocin 23-28 DIRAS family GTPase 3 Homo sapiens 70-74 25193278-11 2014 Overexpression of Sp1 inhibited breast cancer cell migratory and invasive abilities, whereas knockdown of GTP-binding RAS-like 3 (DIRAS3, also known as ARHI, NOEY2) attenuated the inhibitory effects. Guanosine Triphosphate 106-109 DIRAS family GTPase 3 Homo sapiens 152-156 25193278-11 2014 Overexpression of Sp1 inhibited breast cancer cell migratory and invasive abilities, whereas knockdown of GTP-binding RAS-like 3 (DIRAS3, also known as ARHI, NOEY2) attenuated the inhibitory effects. Guanosine Triphosphate 106-109 DIRAS family GTPase 3 Homo sapiens 158-163 24676336-6 2014 Both the transfection of pcDNA3.1(+)-ARHI and the application of TSA+DAC induced the expression of ARHI. trichostatin A 65-68 DIRAS family GTPase 3 Homo sapiens 99-103 24769729-4 2014 Our previous studies have implicated autophagy in the survival of dormant ovarian cancer cells and have shown that ARHI is required for autophagy induced by starvation or rapamycin treatment. Sirolimus 171-180 DIRAS family GTPase 3 Homo sapiens 115-119 24879154-3 2014 Functional inhibition of autophagy with choroquine in dormant xenografts that express DIRAS3 significantly delays tumor regrowth after DIRAS3 levels are reduced, suggesting that autophagy sustains dormant ovarian cancer cells. choroquine 40-50 DIRAS family GTPase 3 Homo sapiens 86-92 24879154-3 2014 Functional inhibition of autophagy with choroquine in dormant xenografts that express DIRAS3 significantly delays tumor regrowth after DIRAS3 levels are reduced, suggesting that autophagy sustains dormant ovarian cancer cells. choroquine 40-50 DIRAS family GTPase 3 Homo sapiens 135-141 24676336-6 2014 Both the transfection of pcDNA3.1(+)-ARHI and the application of TSA+DAC induced the expression of ARHI. Decitabine 69-72 DIRAS family GTPase 3 Homo sapiens 99-103 23157514-2 2013 DiRas3 (ARHI, Noey2), a mainly GTP-bound Ras-like protein with an unusual N-terminal extension, is predominantly lost in ovarian and breast cancers. Guanosine Triphosphate 31-34 DIRAS family GTPase 3 Homo sapiens 0-6 23357870-7 2013 Re-expression of ARHI inhibits FAK (Y397) phosphorylation, disrupts focal adhesions and blocks FAK-mediated RhoA signaling, resulting in decreased levels of GTP-RhoA. Guanosine Triphosphate 157-160 DIRAS family GTPase 3 Homo sapiens 17-21 23504001-9 2013 In A2780 cells, HDACIs upregulated ARHI expression only in the presence of trichostatin A (TSA) plus cisplatin. trichostatin A 75-89 DIRAS family GTPase 3 Homo sapiens 35-39 23504001-9 2013 In A2780 cells, HDACIs upregulated ARHI expression only in the presence of trichostatin A (TSA) plus cisplatin. trichostatin A 91-94 DIRAS family GTPase 3 Homo sapiens 35-39 23504001-9 2013 In A2780 cells, HDACIs upregulated ARHI expression only in the presence of trichostatin A (TSA) plus cisplatin. Cisplatin 101-110 DIRAS family GTPase 3 Homo sapiens 35-39 23247805-2 2013 The eukaryotic expression vector, pcDNA3.1-ARHI, was constructed and transfected into the human lung cancer cell line SK-MES-1. sk-mes 118-124 DIRAS family GTPase 3 Homo sapiens 43-47 23247805-3 2013 The biological properties of the resulting ARHI-expressing lung cancer cell line were evaluated using methyl thiazolyl tetrazolium assay, flow cytometry, and a Transwell invasion assay. methyl thiazolyl tetrazolium 102-130 DIRAS family GTPase 3 Homo sapiens 43-47 23247805-5 2013 Compared to the non-transfected SK-MES-1 cells and the cells transfected with the empty pcDNA3.1 plasmid, the ARHI-transfected cells displayed significantly reduced growth rates and decreased viability (P < 0.05). sk-mes 32-38 DIRAS family GTPase 3 Homo sapiens 110-114 23247805-7 2013 Furthermore, it was determined that ARHI is capable of inhibiting the expression of cyclin D1, MMP-1, and MMP-2; however, ARHI promotes the expression of both p27(KIP1) and DAPK1 in SK-MES-1 cells. 2-(N-morpholino)ethanesulfonic acid 185-188 DIRAS family GTPase 3 Homo sapiens 36-40 23247805-7 2013 Furthermore, it was determined that ARHI is capable of inhibiting the expression of cyclin D1, MMP-1, and MMP-2; however, ARHI promotes the expression of both p27(KIP1) and DAPK1 in SK-MES-1 cells. 2-(N-morpholino)ethanesulfonic acid 185-188 DIRAS family GTPase 3 Homo sapiens 122-126 23157514-2 2013 DiRas3 (ARHI, Noey2), a mainly GTP-bound Ras-like protein with an unusual N-terminal extension, is predominantly lost in ovarian and breast cancers. Guanosine Triphosphate 31-34 DIRAS family GTPase 3 Homo sapiens 8-12 23157514-2 2013 DiRas3 (ARHI, Noey2), a mainly GTP-bound Ras-like protein with an unusual N-terminal extension, is predominantly lost in ovarian and breast cancers. Guanosine Triphosphate 31-34 DIRAS family GTPase 3 Homo sapiens 14-19 23157514-10 2013 Serum-induced recruitment of DiRas3 to the plasma membrane depends mainly on its N-terminal extension and less on its C-terminus, bound nucleotide or the presence of Ras-GTP. ras-gtp 166-173 DIRAS family GTPase 3 Homo sapiens 29-35 23600200-2 2013 METHODS: The eukaryotic expression plasmid of ARHI was constructed and transfected into MKN-28 cell with lipofectamine 2000 as pEGFP-ARHI group, transfected with pEGFP-N1 as pEGFP-N1 group, and untreated MKN-28 as control group. Lipofectamine 105-123 DIRAS family GTPase 3 Homo sapiens 46-50 23600200-2 2013 METHODS: The eukaryotic expression plasmid of ARHI was constructed and transfected into MKN-28 cell with lipofectamine 2000 as pEGFP-ARHI group, transfected with pEGFP-N1 as pEGFP-N1 group, and untreated MKN-28 as control group. mkn-28 88-94 DIRAS family GTPase 3 Homo sapiens 46-50 21772049-9 2011 Among the genes uniquely altered by the combination of panobinostat and decitabine were VHL, TCEB1, WT1, and DIRAS3. Panobinostat 55-67 DIRAS family GTPase 3 Homo sapiens 109-115 21643014-11 2012 In EGF-stimulated ovarian cancer cells, re-expression of ARHI inhibited FAK(Y397) and Src(Y416) phosphorylation, disrupted focal adhesions, and blocked FAK-mediated RhoA signaling, resulting in decreased levels of GTP-RhoA. Guanosine Triphosphate 214-217 DIRAS family GTPase 3 Homo sapiens 57-61 21772049-9 2011 Among the genes uniquely altered by the combination of panobinostat and decitabine were VHL, TCEB1, WT1, and DIRAS3. Decitabine 72-82 DIRAS family GTPase 3 Homo sapiens 109-115 20068591-0 2010 A genome-wide association study for age-related hearing impairment in the Saami. saami 74-79 DIRAS family GTPase 3 Homo sapiens 36-66 21071579-11 2011 Genistein, a potential nontoxic chemopreventive agent, restores expression of ARHI and may be an important dietary therapeutic agent for treating prostate cancer. Genistein 0-9 DIRAS family GTPase 3 Homo sapiens 78-82 22117988-2 2011 Guanosine-5"-triphosphate (GTP)-binding RAS-like 3 (ARHI) is a tumour suppressor gene that has been found to be downregulated in the prostate cancer cell line PC-3. Guanosine Triphosphate 0-25 DIRAS family GTPase 3 Homo sapiens 52-56 22117988-2 2011 Guanosine-5"-triphosphate (GTP)-binding RAS-like 3 (ARHI) is a tumour suppressor gene that has been found to be downregulated in the prostate cancer cell line PC-3. Guanosine Triphosphate 27-30 DIRAS family GTPase 3 Homo sapiens 52-56 19808968-3 2009 LNCaP-ARhi cell line grew faster than the control line in low concentrations, especially in 1 nmol/L 5alpha-dihydrotestosterone (DHT). Dihydrotestosterone 101-127 DIRAS family GTPase 3 Homo sapiens 6-10 19808968-3 2009 LNCaP-ARhi cell line grew faster than the control line in low concentrations, especially in 1 nmol/L 5alpha-dihydrotestosterone (DHT). Dihydrotestosterone 129-132 DIRAS family GTPase 3 Homo sapiens 6-10 19808968-5 2009 Two hundred forty genes showed >2-fold changes on DHT treatment in LNCaP-ARhi at 4 h time point, whereas only 164 and 52 showed changes in LNCaP-ARmo and LNCaP-pcDNA3.1, respectively. Dihydrotestosterone 53-56 DIRAS family GTPase 3 Homo sapiens 76-80 19808968-6 2009 Many androgen-regulated genes were upregulated in LNCaP-ARhi at 10-fold lower concentration of DHT than in control cells. Dihydrotestosterone 95-98 DIRAS family GTPase 3 Homo sapiens 56-60 19808968-7 2009 DHT (1 nmol/L) increased expression of several cell cycle-associated genes in LNCaP-ARhi cells. Dihydrotestosterone 0-3 DIRAS family GTPase 3 Homo sapiens 84-88 19808968-9 2009 The growth of LNCaP-ARhi cells was also highly sensitive to cyclin-dependent kinase inhibitor, roscovitine, at 1nmol/L DHT. Roscovitine 95-106 DIRAS family GTPase 3 Homo sapiens 20-24 19808968-9 2009 The growth of LNCaP-ARhi cells was also highly sensitive to cyclin-dependent kinase inhibitor, roscovitine, at 1nmol/L DHT. Dihydrotestosterone 119-122 DIRAS family GTPase 3 Homo sapiens 20-24 19047183-12 2009 Together these data indicate that common alleles of GRM7 contribute to an individual"s risk of developing ARHI, possibly through a mechanism of altered susceptibility to glutamate excitotoxicity. Glutamic Acid 170-179 DIRAS family GTPase 3 Homo sapiens 106-110 18286529-12 2008 ARHI and PEG3 expression could be restored by treatment with 5-aza-2"-deoxycytidine and trichostatin A, consistent with the importance of promoter methylation and histone acetylation in regulating expression of both genes. Decitabine 61-83 DIRAS family GTPase 3 Homo sapiens 0-4 18612997-6 2009 The expression of ARHI was obviously re-expressed in some HCC cells, Bel-7402, Bel-7405, QGY-7703 and Hep3B, by a demethylation agent, 5-aza-2"-deoxycytidine (DAC). Decitabine 135-157 DIRAS family GTPase 3 Homo sapiens 18-22 18612997-6 2009 The expression of ARHI was obviously re-expressed in some HCC cells, Bel-7402, Bel-7405, QGY-7703 and Hep3B, by a demethylation agent, 5-aza-2"-deoxycytidine (DAC). Decitabine 159-162 DIRAS family GTPase 3 Homo sapiens 18-22 19033662-6 2008 However, inhibition of ARHI-induced autophagy with chloroquine dramatically reduced regrowth of xenografted tumors upon reduction of ARHI levels, suggesting that autophagy contributed to the survival of dormant cells. Chloroquine 51-62 DIRAS family GTPase 3 Homo sapiens 23-27 19033662-6 2008 However, inhibition of ARHI-induced autophagy with chloroquine dramatically reduced regrowth of xenografted tumors upon reduction of ARHI levels, suggesting that autophagy contributed to the survival of dormant cells. Chloroquine 51-62 DIRAS family GTPase 3 Homo sapiens 133-137 18286529-12 2008 ARHI and PEG3 expression could be restored by treatment with 5-aza-2"-deoxycytidine and trichostatin A, consistent with the importance of promoter methylation and histone acetylation in regulating expression of both genes. trichostatin A 88-102 DIRAS family GTPase 3 Homo sapiens 0-4 17513527-5 2007 METHODS: In the present study, the association between ARHI and polymorphisms in genes that contribute to the defence against reactive oxygen species, including GSTT1, GSTM1 and NAT2, was tested. Reactive Oxygen Species 126-149 DIRAS family GTPase 3 Homo sapiens 55-59 17909436-4 2007 Other genes involved in potassium homeostasis have been suggested to be associated with ARHI and NIHL, and distortion product otoacoustic emission distortions indicative of hearing loss alterations have been found in 35delG carriers. Potassium 24-33 DIRAS family GTPase 3 Homo sapiens 88-92 12874023-15 2003 Treatment with 5-aza-2"-deoxycytidine, a methyltransferase inhibitor, could reverse aberrant hypermethylation of CpG island I, II and III and partially restore ARHI expression in some, but not all of the cell lines. Decitabine 15-37 DIRAS family GTPase 3 Homo sapiens 160-164 16158053-2 2006 Reactivation of ARHI expression in breast cancer cells is associated with increased histone H3 acetylation and decreased lysine 9 methylation of histone H3. Lysine 121-127 DIRAS family GTPase 3 Homo sapiens 16-20 16158053-10 2006 When SKBr3 cells were cotransfected with an ARHI/luciferase reporter and E2F-expression vectors, E2F1 and 4 reduced ARHI promoter activity 2-3-fold, and this reduction could be reversed by TSA treatment. trichostatin A 189-192 DIRAS family GTPase 3 Homo sapiens 44-48 16158053-10 2006 When SKBr3 cells were cotransfected with an ARHI/luciferase reporter and E2F-expression vectors, E2F1 and 4 reduced ARHI promoter activity 2-3-fold, and this reduction could be reversed by TSA treatment. trichostatin A 189-192 DIRAS family GTPase 3 Homo sapiens 116-120 15260731-4 2004 It is found that the yield of bound Ar-I radical complexes is typically 90%-100% and 70%-80% for the initial states associated with the Ar-I-H and Ar-H-I isomers, respectively. ar-i radical 36-48 DIRAS family GTPase 3 Homo sapiens 147-153 16757345-13 2006 When ARHI is expressed from a doxycycline-inducible promoter at more physiological levels, autophagy is induced, rather than apoptosis. Doxycycline 30-41 DIRAS family GTPase 3 Homo sapiens 5-9 15172987-4 2004 High levels of promoter methylation of the ARHI gene, encoding a RAS-related small G-protein, were strongly predictive of good survival in patients who had not received tamoxifen therapy. Tamoxifen 169-178 DIRAS family GTPase 3 Homo sapiens 43-47 14506155-5 2003 EXPERIMENTAL DESIGN: We analyzed ARHI expression in DCIS, invasive breast carcinoma, and adjacent normal breast epithelium from 64 formalin-fixed, paraffin-embedded DCIS specimens by both immunohistochemistry and in situ hybridization. Formaldehyde 131-139 DIRAS family GTPase 3 Homo sapiens 33-37 14506155-5 2003 EXPERIMENTAL DESIGN: We analyzed ARHI expression in DCIS, invasive breast carcinoma, and adjacent normal breast epithelium from 64 formalin-fixed, paraffin-embedded DCIS specimens by both immunohistochemistry and in situ hybridization. Paraffin 147-155 DIRAS family GTPase 3 Homo sapiens 33-37 12874100-0 2003 Reactivation of the silenced and imprinted alleles of ARHI is associated with increased histone H3 acetylation and decreased histone H3 lysine 9 methylation. Lysine 136-142 DIRAS family GTPase 3 Homo sapiens 54-58 12874100-5 2003 Chromatin immunoprecipitation assays revealed that histone H3 lysine 9/18 acetylation levels associated with ARHI in normal cells were significantly higher than those in breast cancer cell lines that lacked ARHI expression. Lysine 62-68 DIRAS family GTPase 3 Homo sapiens 109-113 12874100-5 2003 Chromatin immunoprecipitation assays revealed that histone H3 lysine 9/18 acetylation levels associated with ARHI in normal cells were significantly higher than those in breast cancer cell lines that lacked ARHI expression. Lysine 62-68 DIRAS family GTPase 3 Homo sapiens 207-211 12874023-16 2003 Treatment with 5-aza-2"-deoxycytidine partially reactivated ARHI expression in cell lines with hypermethylation of CpG islands I and II but not in cell lines with partial methylation or hypomethylation of these CpG islands. Decitabine 15-37 DIRAS family GTPase 3 Homo sapiens 60-64 12724231-2 2003 ARHI and Ras share similar GTP/GDP binding domains, but exert opposite functions. Guanosine Triphosphate 27-30 DIRAS family GTPase 3 Homo sapiens 0-4 12771940-1 2003 Our group recently identified Ras homolog member I (ARHI), a novel maternally imprinted tumor suppressor gene that encodes a 26 kDa GTP-binding protein with high homology to Ras and Rap. Guanosine Triphosphate 132-135 DIRAS family GTPase 3 Homo sapiens 52-56 12771940-4 2003 Like Ras, ARHI can bind to GTP with high affinity but has low intrinsic GTPase activity. Guanosine Triphosphate 27-30 DIRAS family GTPase 3 Homo sapiens 10-14 12771940-6 2003 (32)Phosphorus labeling showed that ARHI is maintained in a constitutively activated GTP-bound state in resting cells, possibly because of impaired GTPase activity. Phosphorus 4-14 DIRAS family GTPase 3 Homo sapiens 36-40 12771940-6 2003 (32)Phosphorus labeling showed that ARHI is maintained in a constitutively activated GTP-bound state in resting cells, possibly because of impaired GTPase activity. Guanosine Triphosphate 85-88 DIRAS family GTPase 3 Homo sapiens 36-40 12771940-7 2003 ARHI is associated at the cell membrane through its prenylation at the C-terminal cysteine residue. Cysteine 82-90 DIRAS family GTPase 3 Homo sapiens 0-4 12771940-14 2003 In contrast to most other Ras family members, ARHI has a long N-terminal extension, modest GTPase activity, and constitutive GTP binding in resting cells. Guanosine Triphosphate 91-94 DIRAS family GTPase 3 Homo sapiens 46-50 12724231-2 2003 ARHI and Ras share similar GTP/GDP binding domains, but exert opposite functions. Guanosine Diphosphate 31-34 DIRAS family GTPase 3 Homo sapiens 0-4 12499268-1 2002 ARHI, an imprinted putative tumor suppressor gene, encodes a M(r) 26,000 GTP-binding protein that is 60% homologous to ras and rap but has a dramatically different function. Guanosine Triphosphate 73-76 DIRAS family GTPase 3 Homo sapiens 0-4 9578571-11 1998 The results suggest that the RNase A segment of ARH-I not only provides more effective purine recognition but also counteracts the deleterious effects of Gln-117 and Thr-80 on the pyrimidine site. purine 87-93 DIRAS family GTPase 3 Homo sapiens 48-53 9578571-11 1998 The results suggest that the RNase A segment of ARH-I not only provides more effective purine recognition but also counteracts the deleterious effects of Gln-117 and Thr-80 on the pyrimidine site. Glutamine 154-157 DIRAS family GTPase 3 Homo sapiens 48-53 9578571-11 1998 The results suggest that the RNase A segment of ARH-I not only provides more effective purine recognition but also counteracts the deleterious effects of Gln-117 and Thr-80 on the pyrimidine site. Threonine 166-169 DIRAS family GTPase 3 Homo sapiens 48-53 9578571-11 1998 The results suggest that the RNase A segment of ARH-I not only provides more effective purine recognition but also counteracts the deleterious effects of Gln-117 and Thr-80 on the pyrimidine site. pyrimidine 180-190 DIRAS family GTPase 3 Homo sapiens 48-53