PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 34687760-10 2021 The immobilized lipase showed about 2.7 folds (78%) higher stability than the free enzyme at 50 C. Some divalent metal ions, including Cu2+ (22%), Co2+ (37%), Mg2+ (12%), Hg2+ (11%), and Mn2+ (17%) enhanced the enzyme activity of immobilized enzyme. Carbon Dioxide 148-152 lipase Ricinus communis 16-22 33767251-1 2021 The hydrolysis properties of lipase in castor was evaluated using two different substrate forms (tripalmitic glycerides and trioleic glycerides) to catalyze the reaction under different operational conditions. Glycerides 109-119 lipase Ricinus communis 29-35 33767251-1 2021 The hydrolysis properties of lipase in castor was evaluated using two different substrate forms (tripalmitic glycerides and trioleic glycerides) to catalyze the reaction under different operational conditions. trioleic glycerides 124-143 lipase Ricinus communis 29-35 33767251-2 2021 RcLipase was obtained from castor seeds and results show that RcLipase is a conservative serine lipase with a conserved catalytic center (SDH) and a conserved pentapeptide (GXSXG). Serine 89-95 lipase Ricinus communis 0-8 33767251-2 2021 RcLipase was obtained from castor seeds and results show that RcLipase is a conservative serine lipase with a conserved catalytic center (SDH) and a conserved pentapeptide (GXSXG). Serine 89-95 lipase Ricinus communis 62-70 33767251-2 2021 RcLipase was obtained from castor seeds and results show that RcLipase is a conservative serine lipase with a conserved catalytic center (SDH) and a conserved pentapeptide (GXSXG). Serine 89-95 lipase Ricinus communis 96-102 34687760-10 2021 The immobilized lipase showed about 2.7 folds (78%) higher stability than the free enzyme at 50 C. Some divalent metal ions, including Cu2+ (22%), Co2+ (37%), Mg2+ (12%), Hg2+ (11%), and Mn2+ (17%) enhanced the enzyme activity of immobilized enzyme. Metals 114-119 lipase Ricinus communis 16-22 34687760-10 2021 The immobilized lipase showed about 2.7 folds (78%) higher stability than the free enzyme at 50 C. Some divalent metal ions, including Cu2+ (22%), Co2+ (37%), Mg2+ (12%), Hg2+ (11%), and Mn2+ (17%) enhanced the enzyme activity of immobilized enzyme. cupric ion 136-140 lipase Ricinus communis 16-22 34687760-10 2021 The immobilized lipase showed about 2.7 folds (78%) higher stability than the free enzyme at 50 C. Some divalent metal ions, including Cu2+ (22%), Co2+ (37%), Mg2+ (12%), Hg2+ (11%), and Mn2+ (17%) enhanced the enzyme activity of immobilized enzyme. magnesium ion 160-164 lipase Ricinus communis 16-22 34687760-10 2021 The immobilized lipase showed about 2.7 folds (78%) higher stability than the free enzyme at 50 C. Some divalent metal ions, including Cu2+ (22%), Co2+ (37%), Mg2+ (12%), Hg2+ (11%), and Mn2+ (17%) enhanced the enzyme activity of immobilized enzyme. Manganese(2+) 188-192 lipase Ricinus communis 16-22 16666608-1 1989 The neutral lipase (EC 3.1.1.3) in lipid body membranes isolated from the endosperm of 4 day old castor (Ricinus communis L.) seedlings catalyzes the hydrolysis of [(14)C]trioleoylglycerol, releasing [(14)C]oleic acid for up to 4 hours. Triolein 171-188 lipase Ricinus communis 12-18 16666608-1 1989 The neutral lipase (EC 3.1.1.3) in lipid body membranes isolated from the endosperm of 4 day old castor (Ricinus communis L.) seedlings catalyzes the hydrolysis of [(14)C]trioleoylglycerol, releasing [(14)C]oleic acid for up to 4 hours. Oleic Acid 207-217 lipase Ricinus communis 12-18 16666608-2 1989 However, the addition of Mg-ATP and coenzyme A (CoA), which are present in the cytoplasm of plant cells, caused a progressive inhibition of the neutral lipase such that after 15 minutes, release of [(14)C]oleic acid was almost undetectable. Adenosine Triphosphate 25-31 lipase Ricinus communis 152-158 16666608-3 1989 A fatty acyl CoA synthetase was found in the lipid body membrane which converts [(14)C]oleic acid produced from the lipase reaction to [(14)C]oleoyl-CoA under these conditions. Carbon-14 80-87 lipase Ricinus communis 116-122 16666608-3 1989 A fatty acyl CoA synthetase was found in the lipid body membrane which converts [(14)C]oleic acid produced from the lipase reaction to [(14)C]oleoyl-CoA under these conditions. Oleic Acid 87-97 lipase Ricinus communis 116-122 16666608-3 1989 A fatty acyl CoA synthetase was found in the lipid body membrane which converts [(14)C]oleic acid produced from the lipase reaction to [(14)C]oleoyl-CoA under these conditions. [(14)c]oleoyl-coa 135-152 lipase Ricinus communis 116-122 16666608-4 1989 The concentration of free oleoyl-CoA in the reaction mixture when the lipase was inhibited by 50% was calculated to be about 21 micromolar. oleoyl-coenzyme A 26-36 lipase Ricinus communis 70-76 16666608-7 1989 It is possible that this effect is important In vivo in coordinating lipase activity with fatty acid oxidation. Fatty Acids 90-100 lipase Ricinus communis 69-75 16665429-2 1987 The means by which fat hydrolysis is initiated and controlled in the endosperm of the young seedling are not yet understood, although it is generally assumed that the acid lipase is the enzyme responsible for the conversion of stored triacylglycerols to fatty acids and glycerol. Triglycerides 234-250 lipase Ricinus communis 172-178 16665429-2 1987 The means by which fat hydrolysis is initiated and controlled in the endosperm of the young seedling are not yet understood, although it is generally assumed that the acid lipase is the enzyme responsible for the conversion of stored triacylglycerols to fatty acids and glycerol. Fatty Acids 254-265 lipase Ricinus communis 172-178 16665429-2 1987 The means by which fat hydrolysis is initiated and controlled in the endosperm of the young seedling are not yet understood, although it is generally assumed that the acid lipase is the enzyme responsible for the conversion of stored triacylglycerols to fatty acids and glycerol. Glycerol 241-249 lipase Ricinus communis 172-178 16665429-6 1987 The pattern of appearance of the lipase mirrors that of other enzymes involved in the conversion of fat to sugar. Sugars 107-112 lipase Ricinus communis 33-39 16665429-7 1987 The lipase is stimulated 40-fold by 30 micromolar free Ca(2+) and the activity at pH 7.0 to 7.5 adequately accounts for the known rate of triacylglycerol hydrolysis in vivo. Triglycerides 138-153 lipase Ricinus communis 4-10 16664437-1 1985 The alkaline lipase in the glyoxysomes from the endosperm of young castor bean seedlings, an integral membrane component, was solubilized in deoxycholate:KCl and purified to apparent homogeneity. Deoxycholic Acid 141-153 lipase Ricinus communis 13-19 16664437-1 1985 The alkaline lipase in the glyoxysomes from the endosperm of young castor bean seedlings, an integral membrane component, was solubilized in deoxycholate:KCl and purified to apparent homogeneity. Potassium Chloride 154-157 lipase Ricinus communis 13-19 16659994-6 1977 The appearance of this fatty acid implies that lipase activity (lipolysis) is not strictly coordinated with beta oxidation in this tissue. Fatty Acids 23-33 lipase Ricinus communis 47-53 16661626-4 1981 Two cytochrome reductases and phosphorylcholine glyceride transferase co-sedimented with the alkaline lipase, a known component of the glyoxysome membrane, in sucrose gradient centrifugation of osmotically shocked glyoxysomes. Sucrose 159-166 lipase Ricinus communis 102-108 24414640-4 1978 The storage tissues of all these oil seeds except castor bean contained only alkaline lipase activity which increased drastically during germination. Oils 33-36 lipase Ricinus communis 86-92 24414640-8 1978 The glyoxysomal lipase was associated with the organelle membrane, and hydrolyzed only monoglyceride whereas the mitochondrial and membrane-fraction enzymes degraded mono-, di- and triglycerides equally well. Monoglycerides 87-100 lipase Ricinus communis 16-22 24414640-8 1978 The glyoxysomal lipase was associated with the organelle membrane, and hydrolyzed only monoglyceride whereas the mitochondrial and membrane-fraction enzymes degraded mono-, di- and triglycerides equally well. mono-, di- and triglycerides 166-194 lipase Ricinus communis 16-22 24414640-9 1978 Thus, although the lipase in the glyoxysomes had the highest activity, it had to cooperate with lipases in other cellular compartments for the complete hydrolysis of reserve triglycerides. Triglycerides 174-187 lipase Ricinus communis 19-25 16658831-5 1974 Sucrose density gradient centrifugation showed that the alkaline lipase was located mainly in glyoxysomes, with some 30% of the activity in the endoplasmic reticulum. Sucrose 0-7 lipase Ricinus communis 65-71 16658831-6 1974 When glyoxysomes were broken by osmotic shock and exposed to KCl, which solubilizes most of the enzymes, the alkaline lipase remained particulate and was recovered with the glyoxysomal "ghosts" at equilibrium density 1.21 g/cm(3) on the sucrose gradient. Potassium Chloride 61-64 lipase Ricinus communis 118-124 16658831-6 1974 When glyoxysomes were broken by osmotic shock and exposed to KCl, which solubilizes most of the enzymes, the alkaline lipase remained particulate and was recovered with the glyoxysomal "ghosts" at equilibrium density 1.21 g/cm(3) on the sucrose gradient. Sucrose 237-244 lipase Ricinus communis 118-124 16658831-7 1974 Association of the lipase with the gly-oxysomal membrane was supported by the responses to detergents and to butanol. Glycine 35-38 lipase Ricinus communis 19-25 16658831-7 1974 Association of the lipase with the gly-oxysomal membrane was supported by the responses to detergents and to butanol. Butanols 109-116 lipase Ricinus communis 19-25 16658831-8 1974 The alkaline lipase hydrolyzed only monosubstituted glycerols. Glycerol 52-61 lipase Ricinus communis 13-19 21258873-4 2011 Concentrated lipase extracts showed preference to medium-chain triglycerides and fatty acids. Triglycerides 63-76 lipase Ricinus communis 13-19 21258873-4 2011 Concentrated lipase extracts showed preference to medium-chain triglycerides and fatty acids. Fatty Acids 81-92 lipase Ricinus communis 13-19 21258873-6 2011 Higher esterification activities were achieved when the lipase extract was immobilized in sodium alginate and activated coal. Alginates 90-105 lipase Ricinus communis 56-62 18478376-0 2007 Acetone powder from dormant seeds of Ricinus communis L: lipase activity and presence of toxic and allergenic compounds. Acetone 0-7 lipase Ricinus communis 57-63 21114629-4 2011 Triacylglycerol lipase activity was enriched 74-fold from crude latex of Vasconcellea heilbornii to a specific activity (SA) of 57 mumol min(-1) mg(-1) on long-chain triacylglycerol (olive oil). sa 121-123 lipase Ricinus communis 16-22 21114629-4 2011 Triacylglycerol lipase activity was enriched 74-fold from crude latex of Vasconcellea heilbornii to a specific activity (SA) of 57 mumol min(-1) mg(-1) on long-chain triacylglycerol (olive oil). long-chain 155-165 lipase Ricinus communis 16-22 21114629-4 2011 Triacylglycerol lipase activity was enriched 74-fold from crude latex of Vasconcellea heilbornii to a specific activity (SA) of 57 mumol min(-1) mg(-1) on long-chain triacylglycerol (olive oil). Triglycerides 166-181 lipase Ricinus communis 16-22 21114629-8 2011 The protein fraction was incubated with the lipase inhibitor [(14) C]tetrahydrolipstatin, and a 45 kDa protein radiolabeled by the inhibitor was identified as being a putative lipase. [(14) c]tetrahydrolipstatin 61-88 lipase Ricinus communis 44-50 21114629-8 2011 The protein fraction was incubated with the lipase inhibitor [(14) C]tetrahydrolipstatin, and a 45 kDa protein radiolabeled by the inhibitor was identified as being a putative lipase. [(14) c]tetrahydrolipstatin 61-88 lipase Ricinus communis 176-182 19291579-3 2009 Afterwards, the influence on lipolysis of castor oil of free lipases and immobilized lipase derivatives have been studied in the case of production of ricinoleic acid. Castor Oil 42-52 lipase Ricinus communis 61-67 18478376-1 2007 The influence of several factors on the hydrolytic activity of lipase, present in the acetone powder from dormant castor seeds (Ricinus communis) was evaluated. Acetone 86-93 lipase Ricinus communis 63-69 18478376-3 2007 The best reaction conditions were an acid medium, Triton X-100 as the emulsifying agent and a temperature of 30 degrees C. The lipase activity of the acetone powder of different castor oil genotypes showed great variability and storage stability of up to 90%. Octoxynol 50-62 lipase Ricinus communis 127-133 18478376-3 2007 The best reaction conditions were an acid medium, Triton X-100 as the emulsifying agent and a temperature of 30 degrees C. The lipase activity of the acetone powder of different castor oil genotypes showed great variability and storage stability of up to 90%. Acetone 150-157 lipase Ricinus communis 127-133 18478376-3 2007 The best reaction conditions were an acid medium, Triton X-100 as the emulsifying agent and a temperature of 30 degrees C. The lipase activity of the acetone powder of different castor oil genotypes showed great variability and storage stability of up to 90%. Castor Oil 178-188 lipase Ricinus communis 127-133