PMID-sentid	Pub_year	Sent_text	comp_official_name	comp_offset	protein_name	organism	prot_offset
28239415-8	2017	Delaying expression of the assembled metabolic modules using the copper-inducible CUP1 promoter resulted in a 1.6-fold increase in the exponential-phase growth rate and a twofold increase in productivity in the post-exponential phase.	Copper	65-71	metallothionein CUP1	Saccharomyces cerevisiae S288C	82-86
27074556-6	2016	Ethanol-mediated gains of YRF1 and CUP1 genes were the most accented.	Ethanol	0-7	metallothionein CUP1	Saccharomyces cerevisiae S288C	35-39
26112740-9	2015	The galactose-inducible GAL1 promoter provided the highest GFP expression on galactose, and the copper-inducible CUP1 promoter provided the highest induced GFP expression following the diauxic shift.	Copper	96-102	metallothionein CUP1	Saccharomyces cerevisiae S288C	113-117
26392044-6	2015	Utilizing 3vGFP, we further engineered a less leaky Cu(2+)-inducible promoter based on CUP1.	cupric ion	52-58	metallothionein CUP1	Saccharomyces cerevisiae S288C	87-91
26392044-7	2015	The basal expression level of the new promoter was approximately 61% below the wild-type CUP1 promoter, thus expanding the absolute range of Cu(2+)-based gene control.	cupric ion	141-147	metallothionein CUP1	Saccharomyces cerevisiae S288C	89-93
25382639-5	2015	A copper resistance gene (CUP1) was used as the selection marker for yeast transformation.	Copper	2-8	metallothionein CUP1	Saccharomyces cerevisiae S288C	26-30
25382639-6	2015	Because variants of transformants containing different copy numbers at the CUP1 locus can be readily selected after growth in the presence of elevated copper levels, we suggest that this system would prove useful in the generation of tandemly iterated gene clusters.	Copper	151-157	metallothionein CUP1	Saccharomyces cerevisiae S288C	75-79
26083994-0	2015	Yeast CUP1 protects HeLa cells against copper-induced stress.	Copper	39-45	metallothionein CUP1	Saccharomyces cerevisiae S288C	6-10
26083994-4	2015	The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress.	Copper	69-75	metallothionein CUP1	Saccharomyces cerevisiae S288C	53-57
26083994-4	2015	The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress.	Copper	114-120	metallothionein CUP1	Saccharomyces cerevisiae S288C	53-57
26083994-8	2015	The data indicated that overexpression of yeast CUP1 in HeLa cells played a protective role against copper-induced stress, leading to increased cellular viability (P<0.05) and decreased ROS production (P<0.05).	Copper	100-106	metallothionein CUP1	Saccharomyces cerevisiae S288C	48-52
26083994-8	2015	The data indicated that overexpression of yeast CUP1 in HeLa cells played a protective role against copper-induced stress, leading to increased cellular viability (P<0.05) and decreased ROS production (P<0.05).	Reactive Oxygen Species	189-192	metallothionein CUP1	Saccharomyces cerevisiae S288C	48-52
26083994-10	2015	We found that overexpression of yeast CUP1 protected HeLa cells against copper stress.	Copper	72-78	metallothionein CUP1	Saccharomyces cerevisiae S288C	38-42
25519894-10	2015	Analyses indicated that mutations in all four genes, as well as CUP1-1 copy number, contributed significantly to explaining variation in copper tolerance.	Copper	137-143	metallothionein CUP1	Saccharomyces cerevisiae S288C	64-68
25265503-0	2014	Transgenic mice expressing yeast CUP1 exhibit increased copper utilization from feeds.	Copper	56-62	metallothionein CUP1	Saccharomyces cerevisiae S288C	33-37
25265503-8	2014	These results demonstrate that the introduction of the salivary CUP1 transgene into animals offers a possible approach to increase the utilization efficiency of copper and decrease the fecal copper contents.	Copper	191-197	metallothionein CUP1	Saccharomyces cerevisiae S288C	64-68
25236733-2	2014	In the yeast Saccharomyces cerevisiae, most strains contain tandemly duplicated copies of CUP1, a gene that encodes a copper-binding metallothionein.	Copper	118-124	metallothionein CUP1	Saccharomyces cerevisiae S288C	90-94
25265503-4	2014	Considering that the S. cerevisiae CUP1 gene encodes a Cys-rich protein that can bind copper as specifically as copper chelatin in yeast, we therefore constructed a transgene plasmid containing the CUP1 gene regulated for specific expression in the salivary glands by a promoter of gene coding pig parotid secretory protein.	Cysteine	55-58	metallothionein CUP1	Saccharomyces cerevisiae S288C	35-39
25265503-4	2014	Considering that the S. cerevisiae CUP1 gene encodes a Cys-rich protein that can bind copper as specifically as copper chelatin in yeast, we therefore constructed a transgene plasmid containing the CUP1 gene regulated for specific expression in the salivary glands by a promoter of gene coding pig parotid secretory protein.	Cysteine	55-58	metallothionein CUP1	Saccharomyces cerevisiae S288C	198-202
25265503-4	2014	Considering that the S. cerevisiae CUP1 gene encodes a Cys-rich protein that can bind copper as specifically as copper chelatin in yeast, we therefore constructed a transgene plasmid containing the CUP1 gene regulated for specific expression in the salivary glands by a promoter of gene coding pig parotid secretory protein.	Copper	86-92	metallothionein CUP1	Saccharomyces cerevisiae S288C	35-39
25265503-4	2014	Considering that the S. cerevisiae CUP1 gene encodes a Cys-rich protein that can bind copper as specifically as copper chelatin in yeast, we therefore constructed a transgene plasmid containing the CUP1 gene regulated for specific expression in the salivary glands by a promoter of gene coding pig parotid secretory protein.	Copper	86-92	metallothionein CUP1	Saccharomyces cerevisiae S288C	198-202
25265503-4	2014	Considering that the S. cerevisiae CUP1 gene encodes a Cys-rich protein that can bind copper as specifically as copper chelatin in yeast, we therefore constructed a transgene plasmid containing the CUP1 gene regulated for specific expression in the salivary glands by a promoter of gene coding pig parotid secretory protein.	Copper	112-118	metallothionein CUP1	Saccharomyces cerevisiae S288C	35-39
25265503-4	2014	Considering that the S. cerevisiae CUP1 gene encodes a Cys-rich protein that can bind copper as specifically as copper chelatin in yeast, we therefore constructed a transgene plasmid containing the CUP1 gene regulated for specific expression in the salivary glands by a promoter of gene coding pig parotid secretory protein.	Copper	112-118	metallothionein CUP1	Saccharomyces cerevisiae S288C	198-202
25265503-5	2014	Transgenic CUP1 was highly expressed in the parotid and submandibular salivary glands and secreted in saliva as a 9-kDa copper-chelating protein.	Copper	120-126	metallothionein CUP1	Saccharomyces cerevisiae S288C	11-15
25265503-8	2014	These results demonstrate that the introduction of the salivary CUP1 transgene into animals offers a possible approach to increase the utilization efficiency of copper and decrease the fecal copper contents.	Copper	161-167	metallothionein CUP1	Saccharomyces cerevisiae S288C	64-68
23358723-4	2013	The chromosomal rearrangements in EC strains result in segmental duplications in chromosomes 7 and 8, which increase the copy number of genes involved in copper regulation, including the crucial transcriptional activator CUP2 and the metallothionein CUP1.	Copper	154-160	metallothionein CUP1	Saccharomyces cerevisiae S288C	250-254
24471920-1	2014	In yeast, Ace1p-dependent induction of CUP1 is responsible for protecting cells from copper toxicity.	Copper	85-91	metallothionein CUP1	Saccharomyces cerevisiae S288C	39-43
23307895-4	2013	We optimized a CNV detection assay based on a reporter cassette containing the SFA1 and CUP1 genes that confer gene dosage-dependent tolerance to formaldehyde and copper, respectively.	Formaldehyde	146-158	metallothionein CUP1	Saccharomyces cerevisiae S288C	88-92
23307895-4	2013	We optimized a CNV detection assay based on a reporter cassette containing the SFA1 and CUP1 genes that confer gene dosage-dependent tolerance to formaldehyde and copper, respectively.	Copper	163-169	metallothionein CUP1	Saccharomyces cerevisiae S288C	88-92
24298060-5	2014	Chromosome gain was measured by selecting and characterizing copper-resistant colonies that emerged due to increased copies of the metallothionein gene CUP1.	Copper	61-67	metallothionein CUP1	Saccharomyces cerevisiae S288C	152-156
22648686-2	2012	ALD6 encoding an aldehyde dehydrogenases of the indigenous yeast was replaced by a GPD1 and CUP1 gene cassette, which are responsible for NAD-dependent glycerol-3-phosphatase dehydrogenase and copper resistance, respectively.	NAD	138-141	metallothionein CUP1	Saccharomyces cerevisiae S288C	92-96
22648686-2	2012	ALD6 encoding an aldehyde dehydrogenases of the indigenous yeast was replaced by a GPD1 and CUP1 gene cassette, which are responsible for NAD-dependent glycerol-3-phosphatase dehydrogenase and copper resistance, respectively.	Copper	193-199	metallothionein CUP1	Saccharomyces cerevisiae S288C	92-96
22790396-0	2012	Amplification of the CUP1 gene is associated with evolution of copper tolerance in Saccharomyces cerevisiae.	Copper	63-69	metallothionein CUP1	Saccharomyces cerevisiae S288C	21-25
22790396-9	2012	Our results implicate CUP1 in protection of the evolved S. cerevisiae cells against Cu toxicity.	Copper	84-86	metallothionein CUP1	Saccharomyces cerevisiae S288C	22-26
22237983-6	2012	The results showed that the CUP1 gene of Saccharomyces cerevisiae elevated copper resistance of C. glycerinogenes.	Copper	75-81	metallothionein CUP1	Saccharomyces cerevisiae S288C	28-32
22609398-2	2012	CUP1 encodes a cysteine-rich, copper-binding metallothionein that protects cells against copper toxicity through its ability to sequester copper.	Cysteine	15-23	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
22609398-2	2012	CUP1 encodes a cysteine-rich, copper-binding metallothionein that protects cells against copper toxicity through its ability to sequester copper.	Copper	30-36	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
22609398-2	2012	CUP1 encodes a cysteine-rich, copper-binding metallothionein that protects cells against copper toxicity through its ability to sequester copper.	Copper	89-95	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
22609398-2	2012	CUP1 encodes a cysteine-rich, copper-binding metallothionein that protects cells against copper toxicity through its ability to sequester copper.	Copper	89-95	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
22237983-7	2012	The C. glycerinogenes transformed with recombinant vector pGUC, obtained from introducing CUP1 gene into plasmid pGAPZU, could resist 21 mM copper, while the minimum inhibitory concentration (MIC) of wild type was 18 mM in solid YEPD medium.	Copper	140-146	metallothionein CUP1	Saccharomyces cerevisiae S288C	90-94
20839184-0	2010	Evidence of native metal-S(2-)-metallothionein complexes confirmed by the analysis of Cup1 divalent-metal-ion binding properties.	Metals	19-24	metallothionein CUP1	Saccharomyces cerevisiae S288C	86-90
23019066-7	2012	Nevertheless, expression variation in genes like Cup1, important to surviving copper stress, was linked with variation in mitotic fitness and in the breadth of differential expression across the genome.	Copper	78-84	metallothionein CUP1	Saccharomyces cerevisiae S288C	49-53
22365074-0	2012	Copper deprivation modulates CTR1 and CUP1 expression and enhances cisplatin cytotoxicity in Saccharomyces cerevisiae.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	38-42
22365074-7	2012	Also, CDDP further lowered copper deprivation-induced changes in CUP1 metallothionein levels, SOD activity and GSH levels.	Cisplatin	6-10	metallothionein CUP1	Saccharomyces cerevisiae S288C	65-69
22365074-7	2012	Also, CDDP further lowered copper deprivation-induced changes in CUP1 metallothionein levels, SOD activity and GSH levels.	Copper	27-33	metallothionein CUP1	Saccharomyces cerevisiae S288C	65-69
21409410-4	2011	Among 90 genes affected by both AgNPs and Ag-ions, metalloprotein mediating high resistance to copper (CUP1-1 and CUP1-2) were strongly induced by AgNPs (~45-folds) and Ag-ions (~22-folds), respectively.	Copper	95-101	metallothionein CUP1	Saccharomyces cerevisiae S288C	103-109
21180717-2	2011	The Cu(+)-binding curves obtained for both regulators as well as for the copper storage proteins Cup1 and Crs5 show that free copper(i) is maintained in a narrow window inside yeast.	Copper	73-79	metallothionein CUP1	Saccharomyces cerevisiae S288C	97-101
21180717-2	2011	The Cu(+)-binding curves obtained for both regulators as well as for the copper storage proteins Cup1 and Crs5 show that free copper(i) is maintained in a narrow window inside yeast.	Copper	126-132	metallothionein CUP1	Saccharomyces cerevisiae S288C	97-101
20839184-4	2010	Because, although practically universal, the highest S(2-) content is incorporated by copper-thioneins when coordinating divalent metal ions, we adapted the Saccharomyces cerevisiae Cup1 protein, which is the most paradigmatic copper-thionein, as an experimental model.	Metals	130-135	metallothionein CUP1	Saccharomyces cerevisiae S288C	182-186
20839184-5	2010	Most significantly, native Cd-Cup1 complexes were purified and fully spectroscopically and spectrometrically characterized from the 301N mutant yeast strain, which allows Cup1 synthesis even in the absence of copper.	Copper	209-215	metallothionein CUP1	Saccharomyces cerevisiae S288C	30-34
20839184-6	2010	These results undoubtedly revealed the presence of a Cd-S(2-)-Cup1 species in native preparations, which were only recovered when carefully avoiding the use of ion-exchange chromatography in the purification protocol.	Cadmium	53-57	metallothionein CUP1	Saccharomyces cerevisiae S288C	62-66
20839184-7	2010	Furthermore, complete analysis of recombinant (Escherichia coli) Zn-Cup1, Cd-Cup1, and Cu-Cup1 and those complexes that result from Zn/Cd and Zn/Cu replacements in vitro and acidification/renaturalization processes yielded a comprehensive and comparative overview of the metal-binding abilities of Cup1.	Zinc	65-67	metallothionein CUP1	Saccharomyces cerevisiae S288C	68-72
18751932-3	2009	Uniform design and immunoblotting was used to determine an optimal dose of copper which control the expression of ERs through a copper inducible metallothionine promoter (CUP1).	Copper	75-81	metallothionein CUP1	Saccharomyces cerevisiae S288C	171-175
20839184-0	2010	Evidence of native metal-S(2-)-metallothionein complexes confirmed by the analysis of Cup1 divalent-metal-ion binding properties.	metal-s(2-)-metallothionein	19-46	metallothionein CUP1	Saccharomyces cerevisiae S288C	86-90
18512161-2	2009	Yeasts and the yeast Saccharomyces cerevisiae, in particular, possess in their genome tandem repeats of the CUP1 gene coding for a protein (a metallothionein) capable of capturing and binding toxic elements such as copper ions.	Copper	215-221	metallothionein CUP1	Saccharomyces cerevisiae S288C	108-112
18751932-3	2009	Uniform design and immunoblotting was used to determine an optimal dose of copper which control the expression of ERs through a copper inducible metallothionine promoter (CUP1).	Copper	128-134	metallothionein CUP1	Saccharomyces cerevisiae S288C	171-175
18751932-3	2009	Uniform design and immunoblotting was used to determine an optimal dose of copper which control the expression of ERs through a copper inducible metallothionine promoter (CUP1).	metallothionine	145-160	metallothionein CUP1	Saccharomyces cerevisiae S288C	171-175
18528910-4	2008	Copper metallothionein CUP1-1 and CUP1-2 were induced more than 20-fold.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	23-29
18581063-2	2008	Upstream activating sequences of the S. cerevisiae alcohol dehydrogenase II (ADH2) gene promoter (P(U-ADH2)) were used to regulate the expression of FLONS; alpha-acetolactate synthase gene ILV2 was chosen for homologous recombination of I10 to the YSF5 chromosome; copper binding metallothionein (encoded by CUP1) was used for selection of transformants.	Copper	265-271	metallothionein CUP1	Saccharomyces cerevisiae S288C	308-312
18528910-4	2008	Copper metallothionein CUP1-1 and CUP1-2 were induced more than 20-fold.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	23-27
18437993-5	2008	The copper-resistance gene (CUP1-MT1) was cloned into pSH47, which yielded pSH-CUP.	Copper	4-10	metallothionein CUP1	Saccharomyces cerevisiae S288C	28-36
17163970-2	2007	Although considered a secondary copper-resistance agent, we show here that it determines survival under zinc overload in a CUP1-null background.	Copper	32-38	metallothionein CUP1	Saccharomyces cerevisiae S288C	123-127
17163970-3	2007	Its overexpression prevents the deleterious effects exhibited by CUP1-CRS5-null cells when exposed to combined Zn/Cu, as it does the mouse MT1 Zn-thionein, but not Cup1.	Copper	114-116	metallothionein CUP1	Saccharomyces cerevisiae S288C	65-69
17284864-4	2007	The genes underexpressed in K-9 more than in X2180-1A were CUP1-1 and CUP1-2, PHO genes, which may explain the low copper tolerance and low acid phosphatase activity of sake yeast.	Copper	115-121	metallothionein CUP1	Saccharomyces cerevisiae S288C	59-65
17163970-3	2007	Its overexpression prevents the deleterious effects exhibited by CUP1-CRS5-null cells when exposed to combined Zn/Cu, as it does the mouse MT1 Zn-thionein, but not Cup1.	Zinc	111-113	metallothionein CUP1	Saccharomyces cerevisiae S288C	65-69
20020960-0	2007	Role of glutathione in detoxification of copper and cadmium by yeast cells having different abilities to express cup1 protein.	Glutathione	8-19	metallothionein CUP1	Saccharomyces cerevisiae S288C	113-117
17163970-3	2007	Its overexpression prevents the deleterious effects exhibited by CUP1-CRS5-null cells when exposed to combined Zn/Cu, as it does the mouse MT1 Zn-thionein, but not Cup1.	zn-thionein	143-154	metallothionein CUP1	Saccharomyces cerevisiae S288C	65-69
20020960-0	2007	Role of glutathione in detoxification of copper and cadmium by yeast cells having different abilities to express cup1 protein.	Cadmium	52-59	metallothionein CUP1	Saccharomyces cerevisiae S288C	113-117
20020960-4	2007	The yeast cells used in this study have different abilities to produce glutathione and Cup1 protein, the yeast metallothionein homolog encoded by CUP1 gene.	Glutathione	71-82	metallothionein CUP1	Saccharomyces cerevisiae S288C	146-150
20020960-5	2007	It was demonstrated that Cup1 protein plays a dominant role in buffering excess copper, and yeast does not depend on glutathione to reduce copper toxicity whether it possesses single or multiple copies of CUP1.	Copper	80-86	metallothionein CUP1	Saccharomyces cerevisiae S288C	25-29
20020960-7	2007	On the other hand, it was indicated that Cup1 protein is an important cadmium-detoxifying component, and the glutathione system can positively respond to cadmium.	Cadmium	70-77	metallothionein CUP1	Saccharomyces cerevisiae S288C	41-45
20020960-7	2007	On the other hand, it was indicated that Cup1 protein is an important cadmium-detoxifying component, and the glutathione system can positively respond to cadmium.	Glutathione	109-120	metallothionein CUP1	Saccharomyces cerevisiae S288C	41-45
20020960-7	2007	On the other hand, it was indicated that Cup1 protein is an important cadmium-detoxifying component, and the glutathione system can positively respond to cadmium.	Cadmium	154-161	metallothionein CUP1	Saccharomyces cerevisiae S288C	41-45
20020960-8	2007	In yeast containing single or multiple copies of CUP1, glutathione is an indispensable line of defense against cadmium.	Glutathione	55-66	metallothionein CUP1	Saccharomyces cerevisiae S288C	49-53
20020960-8	2007	In yeast containing single or multiple copies of CUP1, glutathione is an indispensable line of defense against cadmium.	Cadmium	111-118	metallothionein CUP1	Saccharomyces cerevisiae S288C	49-53
14612437-4	2004	Although induction of the HSF target gene CUP1 by glucose starvation is dependent on the Snf1 kinase, HSF-dependent heat shock induction of CUP1 is Snf1-independent.	Glucose	50-57	metallothionein CUP1	Saccharomyces cerevisiae S288C	42-46
15992933-2	2005	Our conventional transactivation assay functions with an expression plasmid expressing estrogen receptor alpha (ERalpha) under the control of a copper-inducible CUP1 promoter and a reporter plasmid expressing beta-galactosidase under the control of the vitellogenin estrogen response element (ERE).	Copper	144-150	metallothionein CUP1	Saccharomyces cerevisiae S288C	161-165
15501826-4	2005	In yeast, the CUP1 gene encodes a copper metallothionein that is strongly induced in response to metals and other stress and is subsequently rapidly down-regulated.	Copper	34-40	metallothionein CUP1	Saccharomyces cerevisiae S288C	14-18
15501826-5	2005	Activation of CUP1 is mediated by the copper-responsive transcriptional activator AceI, and also requires the histone acetylase Spt10 for full induction.	Copper	38-44	metallothionein CUP1	Saccharomyces cerevisiae S288C	14-18
15501826-9	2005	In contrast, serine mutations in H2A prevent CUP1 shutdown when combined with spt10 deletions.	Serine	13-19	metallothionein CUP1	Saccharomyces cerevisiae S288C	45-49
15515160-3	2004	When copper ions are present in the sample, the Ace1 protein activates the cup1 promoter located upstream from the gfpuv gene in plasmid pYEX-GFPuv, thus inducing the production of GFPuv.	Copper	5-11	metallothionein CUP1	Saccharomyces cerevisiae S288C	75-79
16377055-3	2006	Here, we investigate the separate domain contribution to the in vivo binding of Zn and Cu and to confer metal tolerance to CUP1-null yeast cells of a plant type 2 MT (QsMT).	Metals	104-109	metallothionein CUP1	Saccharomyces cerevisiae S288C	123-127
16283296-7	2006	Overexpression of the GPD1 gene encoding glycerol-3-phosphate dehydrogenase, ENA1 encoding sodium ion efflux protein, and CUP1 encoding copper metallothionein conferred high salt stress tolerance to yeast cells, and our selection of candidate genes for the creation of stress-tolerant yeast strains based on the transcriptome data was validated.	Copper	136-142	metallothionein CUP1	Saccharomyces cerevisiae S288C	122-126
16283296-7	2006	Overexpression of the GPD1 gene encoding glycerol-3-phosphate dehydrogenase, ENA1 encoding sodium ion efflux protein, and CUP1 encoding copper metallothionein conferred high salt stress tolerance to yeast cells, and our selection of candidate genes for the creation of stress-tolerant yeast strains based on the transcriptome data was validated.	Salts	174-178	metallothionein CUP1	Saccharomyces cerevisiae S288C	122-126
16579462-11	2006	The dominant selection marker, copper-resistance gene expression cassette CUP1-MTI was inserted in plasmid pPM to result in pCPM.	Copper	31-37	metallothionein CUP1	Saccharomyces cerevisiae S288C	74-78
17657345-5	2006	Moreover, Northern blot hybridization studies indicated that Pc-ace1 cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance.	Copper	83-89	metallothionein CUP1	Saccharomyces cerevisiae S288C	116-121
17657345-5	2006	Moreover, Northern blot hybridization studies indicated that Pc-ace1 cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance.	Metals	146-151	metallothionein CUP1	Saccharomyces cerevisiae S288C	116-121
17657345-5	2006	Moreover, Northern blot hybridization studies indicated that Pc-ace1 cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance.	Copper	198-204	metallothionein CUP1	Saccharomyces cerevisiae S288C	116-121
21783616-11	2005	In contrast, at high levels of Cup1, the concentration of these metals falls, inducing ZRT1 expression and favoring cadmium absorption.	Cadmium	116-123	metallothionein CUP1	Saccharomyces cerevisiae S288C	31-35
15849781-6	2005	The widely used S. cerevisiae GAL1 and CUP1 promoters both require the addition of an inducer [galactose and copper(II) ion, respectively] before regulated genes will be expressed.	Galactose	95-104	metallothionein CUP1	Saccharomyces cerevisiae S288C	39-43
15849781-6	2005	The widely used S. cerevisiae GAL1 and CUP1 promoters both require the addition of an inducer [galactose and copper(II) ion, respectively] before regulated genes will be expressed.	cupric ion	109-119	metallothionein CUP1	Saccharomyces cerevisiae S288C	39-43
15833139-9	2005	Strains with fewer copies of the CUP1 loci showed hypersensitivity to sulfomethuron methyl.	sulfomethuron methyl	70-90	metallothionein CUP1	Saccharomyces cerevisiae S288C	33-37
14994274-4	2004	We observed that the deficiency of Sod1 increases the expression of both Cup1 (a metallothionein) and Ycf1 (a vacuolar glutathione S-conjugate pump), proteins involved with protection against cadmium.	Cadmium	192-199	metallothionein CUP1	Saccharomyces cerevisiae S288C	73-77
12480908-7	2002	Sequence analysis of the tahA promoter revealed several motifs that were similar to the conserved motifs found in the copper-regulated metallothionein and Cu, Zn superoxide dismutase genes, CUP1 and SOD1, of Saccharomyces cerevisiae, Neurospora crassa and Candida glabrata.	Copper	118-124	metallothionein CUP1	Saccharomyces cerevisiae S288C	190-194
14618564-3	2003	We used a synthetic promoter library and the copper-regulated CUP1 promoter to generate G6PDH-activities between 0% and 179% of the wild-type level.	Copper	45-51	metallothionein CUP1	Saccharomyces cerevisiae S288C	62-66
14617147-5	2003	From a range of potential Cu-sensitive mutants, cup1Delta cells lacking Cu-metallothionein, and particularly sod1Delta cells lacking Cu, Zn-superoxide dismutase, exhibited diminished heterogeneity.	cu-metallothionein	72-90	metallothionein CUP1	Saccharomyces cerevisiae S288C	48-52
14558144-6	2003	In the first, expression of the luciferase gene is under control of CUP1-promoter, a well known yeast promoter that is inducible by copper ions.	Copper	132-138	metallothionein CUP1	Saccharomyces cerevisiae S288C	68-72
12675559-2	2003	To augment higher plant metal sequestration, the yeast metallothionein (CUP 1) was introduced into tobacco plants.	Metals	24-29	metallothionein CUP1	Saccharomyces cerevisiae S288C	72-77
12675559-6	2003	Despite low mRNA levels, CUP 1 transformants accumulated up to seven times more copper in older versus younger leaves during copper stress.	Copper	80-86	metallothionein CUP1	Saccharomyces cerevisiae S288C	25-30
12675559-6	2003	Despite low mRNA levels, CUP 1 transformants accumulated up to seven times more copper in older versus younger leaves during copper stress.	Copper	125-131	metallothionein CUP1	Saccharomyces cerevisiae S288C	25-30
12675559-9	2003	Using this transgenic approach, yeast CUP 1 expression under nonstressed conditions contributed to copper metal phytoextraction during a subsequent copper challenge.	Copper	99-105	metallothionein CUP1	Saccharomyces cerevisiae S288C	38-43
12675559-9	2003	Using this transgenic approach, yeast CUP 1 expression under nonstressed conditions contributed to copper metal phytoextraction during a subsequent copper challenge.	Metals	106-111	metallothionein CUP1	Saccharomyces cerevisiae S288C	38-43
12675559-9	2003	Using this transgenic approach, yeast CUP 1 expression under nonstressed conditions contributed to copper metal phytoextraction during a subsequent copper challenge.	Copper	148-154	metallothionein CUP1	Saccharomyces cerevisiae S288C	38-43
12558798-2	2003	For example, copper-inducible transcription of the copper metallothionein gene CUP1 occurs independently of basal factor TFIIE in budding yeast.	Copper	13-19	metallothionein CUP1	Saccharomyces cerevisiae S288C	79-83
11073899-4	2000	Here we show, using DAL80 fused to the carbon-regulated GAL1,10 or copper-regulated CUP1 promoter, that GAT1 expression is inversely regulated by the level of DAL80 expression, i.e., as DAL80 expression increases, GAT1 expression decreases.	Carbon	39-45	metallothionein CUP1	Saccharomyces cerevisiae S288C	84-88
12111155-2	2002	A fusion gene for the expression of GTS1, which encodes a putative zinc-finger transcription factor related to occurrence of cell-aggregation, was constructed under the control of the copper ion-inducible CUP1 promoter from the yeast metallothionein gene.	Copper	184-190	metallothionein CUP1	Saccharomyces cerevisiae S288C	205-209
12192040-2	2002	CUP1 encodes a metallothionein required for cell growth at high copper concentrations.	Copper	64-70	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
12192040-3	2002	Induction of CUP1 with copper resulted in targeted acetylation of both H3 and H4 at the CUP1 promoter.	Copper	23-29	metallothionein CUP1	Saccharomyces cerevisiae S288C	13-17
12192040-3	2002	Induction of CUP1 with copper resulted in targeted acetylation of both H3 and H4 at the CUP1 promoter.	Copper	23-29	metallothionein CUP1	Saccharomyces cerevisiae S288C	88-92
11921088-7	2002	A mutant GIN11M86 sequence was selected and fused to the CUP1 promoter for the counter-selection on a copper-containing medium.	Copper	102-108	metallothionein CUP1	Saccharomyces cerevisiae S288C	57-61
11134341-1	2001	The yeast CUP1 gene is activated by the copper-dependent binding of the transcriptional activator, Ace1p.	Copper	40-46	metallothionein CUP1	Saccharomyces cerevisiae S288C	10-14
11297016-0	2001	Induction for the expression of yeast metallothionein gene, CUP1, by cobalt.	Cobalt	69-75	metallothionein CUP1	Saccharomyces cerevisiae S288C	60-64
11297016-1	2001	Induction for the expression of the metallothionein gene, CUP1, in the yeast Saccharomyces cerevisiae by cobalt was examined using a reporter gene with the promoter of this gene fused to the coding region of lacZ.	Cobalt	105-111	metallothionein CUP1	Saccharomyces cerevisiae S288C	58-62
11297016-4	2001	It has been reported that the induction for the transcription of CUP1 by copper and silver is mediated by the Ace1 transcription factor.	Copper	73-79	metallothionein CUP1	Saccharomyces cerevisiae S288C	65-69
11297016-6	2001	These results suggest the presence of a novel cobalt-specific transcription factor for the CUP1 gene.	Cobalt	46-52	metallothionein CUP1	Saccharomyces cerevisiae S288C	91-95
11073899-4	2000	Here we show, using DAL80 fused to the carbon-regulated GAL1,10 or copper-regulated CUP1 promoter, that GAT1 expression is inversely regulated by the level of DAL80 expression, i.e., as DAL80 expression increases, GAT1 expression decreases.	Copper	67-73	metallothionein CUP1	Saccharomyces cerevisiae S288C	84-88
10922376-8	2000	Elevated copper levels induced the expression of the metallothioneins CUP1 and CRS5 and two genes, FET3 and FTR1, in the iron uptake system.	Copper	9-15	metallothionein CUP1	Saccharomyces cerevisiae S288C	70-74
11286339-3	2000	These strains contain plasmids with the Cu2+-inducible promoter of the CUP1-gene from Saccharomyces cerevisiae fused to the lacZ-gene from E. coli.	cupric ion	40-44	metallothionein CUP1	Saccharomyces cerevisiae S288C	71-75
11015728-7	2000	We placed yEGFP3-CLN2(PEST) under the control of the CUP1 promoter, which is induced only transiently by copper.	Copper	105-111	metallothionein CUP1	Saccharomyces cerevisiae S288C	53-57
11286339-4	2000	On this sensor the CUP1 promoter is first induced by the Cu2+-containing probe and subsequently lactose is used as a deputy substrate to make the measurement.	cupric ion	57-61	metallothionein CUP1	Saccharomyces cerevisiae S288C	19-23
11286339-4	2000	On this sensor the CUP1 promoter is first induced by the Cu2+-containing probe and subsequently lactose is used as a deputy substrate to make the measurement.	Lactose	96-103	metallothionein CUP1	Saccharomyces cerevisiae S288C	19-23
10806427-0	2000	The CUP1 promoter of Saccharomyces cerevisiae is inducible by copper in Pichia pastoris.	Copper	62-68	metallothionein CUP1	Saccharomyces cerevisiae S288C	4-8
10694579-1	2000	Nitric oxide (NO) was found to inhibit the copper-dependent induction of the yeast CUP1 gene.	Nitric Oxide	0-12	metallothionein CUP1	Saccharomyces cerevisiae S288C	83-87
10694579-1	2000	Nitric oxide (NO) was found to inhibit the copper-dependent induction of the yeast CUP1 gene.	Copper	43-49	metallothionein CUP1	Saccharomyces cerevisiae S288C	83-87
10694579-2	2000	This effect is attributable to an inhibition of the copper-responsive CUP1 transcriptional activator Ace1.	Copper	52-58	metallothionein CUP1	Saccharomyces cerevisiae S288C	70-74
10694579-4	2000	Moreover, it is proposed that demetallated Ace1 is proteolytically degraded in the cell, resulting in a prolonged inhibition of copper-dependent CUP1 induction.	Copper	128-134	metallothionein CUP1	Saccharomyces cerevisiae S288C	145-149
10628969-8	2000	In addition, these mutations are capable of suppressing certain alterations in the conserved PyAG trinucleotide at the 3" splice junction, as detected by an ACT1-CUP1 splicing reporter system.	pyag trinucleotide	93-111	metallothionein CUP1	Saccharomyces cerevisiae S288C	162-166
8702498-0	1996	Enhanced effectiveness of copper ion buffering by CUP1 metallothionein compared with CRS5 metallothionein in Saccharomyces cerevisiae.	Copper	26-32	metallothionein CUP1	Saccharomyces cerevisiae S288C	50-54
10816730-2	1999	Expression of the CUP1 promoter/lacZ fusion gene was inhibited by all heavy metal ions tested, especially Cd2+ and Mn2+.	Metals	76-81	metallothionein CUP1	Saccharomyces cerevisiae S288C	18-22
10816730-2	1999	Expression of the CUP1 promoter/lacZ fusion gene was inhibited by all heavy metal ions tested, especially Cd2+ and Mn2+.	Manganese(2+)	115-119	metallothionein CUP1	Saccharomyces cerevisiae S288C	18-22
10816730-8	1999	Inhibitory concentrations of Co2+, Ni2+ and Zn2+ on expression of the CUP1p/lacZ fusion gene were at least one order of magnitude higher than that of Cd2+ and Mn2+.	Cobalt(2+)	29-33	metallothionein CUP1	Saccharomyces cerevisiae S288C	70-75
10816730-8	1999	Inhibitory concentrations of Co2+, Ni2+ and Zn2+ on expression of the CUP1p/lacZ fusion gene were at least one order of magnitude higher than that of Cd2+ and Mn2+.	Nickel(2+)	35-39	metallothionein CUP1	Saccharomyces cerevisiae S288C	70-75
10816730-8	1999	Inhibitory concentrations of Co2+, Ni2+ and Zn2+ on expression of the CUP1p/lacZ fusion gene were at least one order of magnitude higher than that of Cd2+ and Mn2+.	Zinc	44-48	metallothionein CUP1	Saccharomyces cerevisiae S288C	70-75
10816730-8	1999	Inhibitory concentrations of Co2+, Ni2+ and Zn2+ on expression of the CUP1p/lacZ fusion gene were at least one order of magnitude higher than that of Cd2+ and Mn2+.	Manganese(2+)	159-163	metallothionein CUP1	Saccharomyces cerevisiae S288C	70-75
9599102-2	1998	In Saccharomyces cerevisiae, the nutritional copper sensor Mac1p regulates the copper-dependent expression of the high affinity Cu(I) uptake genes CTR1, CTR3, and FRE1, while the toxic copper sensor Ace1p regulates the transcriptional activation of the detoxification genes CUP1, CRS5, and SOD1 in response to copper.	Copper	79-85	metallothionein CUP1	Saccharomyces cerevisiae S288C	274-278
9599102-2	1998	In Saccharomyces cerevisiae, the nutritional copper sensor Mac1p regulates the copper-dependent expression of the high affinity Cu(I) uptake genes CTR1, CTR3, and FRE1, while the toxic copper sensor Ace1p regulates the transcriptional activation of the detoxification genes CUP1, CRS5, and SOD1 in response to copper.	Copper	79-85	metallothionein CUP1	Saccharomyces cerevisiae S288C	274-278
9599102-4	1998	Upon addition of CuSO4, mRNA levels of CTR3 were rapidly reduced to eightfold the original basal level whereas the Ace1p-mediated transcriptional activation of CUP1 was rapid and potent but transient.	Copper Sulfate	17-22	metallothionein CUP1	Saccharomyces cerevisiae S288C	160-164
9599102-6	1998	In vivo dimethyl sulfate footprinting analysis of the CUP1 promoter demonstrated transient occupation of the metal response elements by Ace1p which paralleled CUP1 mRNA expression.	dimethyl sulfate	8-24	metallothionein CUP1	Saccharomyces cerevisiae S288C	54-58
9599102-6	1998	In vivo dimethyl sulfate footprinting analysis of the CUP1 promoter demonstrated transient occupation of the metal response elements by Ace1p which paralleled CUP1 mRNA expression.	Metals	109-114	metallothionein CUP1	Saccharomyces cerevisiae S288C	54-58
9599102-7	1998	Analysis of a Mac1p mutant, refractile for copper-dependent repression of the Cu(I) transport genes, showed an aberrant pattern of CUP1 expression and copper sensitivity.	Copper	43-49	metallothionein CUP1	Saccharomyces cerevisiae S288C	131-135
8917464-3	1996	LDTI was expressed in the yeast Saccharomyces cerevisiae under the control of the copper-inducible CUP1 promoter and fused to the invertase signal sequence (SUC2).	Copper	82-88	metallothionein CUP1	Saccharomyces cerevisiae S288C	99-103
8798765-10	1996	In contrast, the signaling and activation of mating pheromone (FUS1) and copper-responsive (CUP1) promoter activity were not affected by dioxygen stress, while genes encoding other anti-oxidant enzymes (SOD2, CTT1 and CTA1) were strongly induced.	Copper	73-79	metallothionein CUP1	Saccharomyces cerevisiae S288C	92-96
9716404-3	1998	CUP1 transcripts made by DeltaCTD Pol II initiated correctly and some extended past the normal poly(A) site yielding a novel dicistronic mRNA.	Poly A	95-102	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
9716404-6	1998	The upstream-activating sequence (UAS) of the CUP1 promoter was sufficient to drive Cu2+ inducible transcription without Srb4 and heat shock inducible transcription without the CTD.	cupric ion	84-88	metallothionein CUP1	Saccharomyces cerevisiae S288C	46-50
9599102-2	1998	In Saccharomyces cerevisiae, the nutritional copper sensor Mac1p regulates the copper-dependent expression of the high affinity Cu(I) uptake genes CTR1, CTR3, and FRE1, while the toxic copper sensor Ace1p regulates the transcriptional activation of the detoxification genes CUP1, CRS5, and SOD1 in response to copper.	Copper	45-51	metallothionein CUP1	Saccharomyces cerevisiae S288C	274-278
9599102-2	1998	In Saccharomyces cerevisiae, the nutritional copper sensor Mac1p regulates the copper-dependent expression of the high affinity Cu(I) uptake genes CTR1, CTR3, and FRE1, while the toxic copper sensor Ace1p regulates the transcriptional activation of the detoxification genes CUP1, CRS5, and SOD1 in response to copper.	Copper	79-85	metallothionein CUP1	Saccharomyces cerevisiae S288C	274-278
9599102-2	1998	In Saccharomyces cerevisiae, the nutritional copper sensor Mac1p regulates the copper-dependent expression of the high affinity Cu(I) uptake genes CTR1, CTR3, and FRE1, while the toxic copper sensor Ace1p regulates the transcriptional activation of the detoxification genes CUP1, CRS5, and SOD1 in response to copper.	cuprous ion	128-133	metallothionein CUP1	Saccharomyces cerevisiae S288C	274-278
9153234-7	1997	Both Fre1p and Fre2p enzymes are functionally significant for copper uptake, as monitored by the accumulation of the copper-regulated CUP1 and CTR1 mRNAs in fre1Delta, fre2Delta, and fre1Deltafre2Delta mutant strains.	Copper	62-68	metallothionein CUP1	Saccharomyces cerevisiae S288C	134-138
9153234-7	1997	Both Fre1p and Fre2p enzymes are functionally significant for copper uptake, as monitored by the accumulation of the copper-regulated CUP1 and CTR1 mRNAs in fre1Delta, fre2Delta, and fre1Deltafre2Delta mutant strains.	Copper	117-123	metallothionein CUP1	Saccharomyces cerevisiae S288C	134-138
8702498-2	1996	We demonstrate that CUP1 plays the dominant role in copper detoxification.	Copper	52-58	metallothionein CUP1	Saccharomyces cerevisiae S288C	20-24
8702498-3	1996	A single copy of CUP1 was far more effective in conferring copper resistance than was CRS5.	Copper	59-65	metallothionein CUP1	Saccharomyces cerevisiae S288C	17-21
8702498-4	1996	The CUP1 promoter contributes to this resistance; in a promoter exchange experiment, the Crs5 MT conferred strong copper resistance when its expression was driven by the CUP1 promoter, and conversely, the CRS5 promoter reduced the effectiveness of Cup1 MT.	Copper	114-120	metallothionein CUP1	Saccharomyces cerevisiae S288C	4-8
8702498-6	1996	The CUP1 coding sequences also contribute to copper tolerance, presumably reflecting the enhanced binding avidity of Cup1 MT for Cu(I) ions.	Copper	45-51	metallothionein CUP1	Saccharomyces cerevisiae S288C	4-8
8702498-6	1996	The CUP1 coding sequences also contribute to copper tolerance, presumably reflecting the enhanced binding avidity of Cup1 MT for Cu(I) ions.	Copper	45-51	metallothionein CUP1	Saccharomyces cerevisiae S288C	117-121
8702498-6	1996	The CUP1 coding sequences also contribute to copper tolerance, presumably reflecting the enhanced binding avidity of Cup1 MT for Cu(I) ions.	cuprous ion	129-134	metallothionein CUP1	Saccharomyces cerevisiae S288C	4-8
8702498-6	1996	The CUP1 coding sequences also contribute to copper tolerance, presumably reflecting the enhanced binding avidity of Cup1 MT for Cu(I) ions.	cuprous ion	129-134	metallothionein CUP1	Saccharomyces cerevisiae S288C	117-121
8702498-7	1996	In studies with the bathocuproine Cu(I) chelator, the Cu(I) ions bound to Crs5 were kinetically more labile than the Cu(I) binding to Cup1.	bathocuproine	20-33	metallothionein CUP1	Saccharomyces cerevisiae S288C	134-138
8654982-3	1996	Expression features are based on the CUP1 yeast metallothionein gene promoter, which can be tightly modulated by copper.	Copper	113-119	metallothionein CUP1	Saccharomyces cerevisiae S288C	37-41
8615757-3	1996	Two yeast expression vectors have been prepared, YEpR1 and YEpR2, which code for 5alpha-R type 1 and 5alpha-R type 2 respectively; they contain the copper-responsive yeast metallothionein promoter (CUP1) upstream of the ubiquitin coding sequence, and the full-length rat 5alpha-R type 1 or 5alpha-R type 2 cDNAs in frame to the 3" end of the ubiquitin cDNA.	Copper	148-154	metallothionein CUP1	Saccharomyces cerevisiae S288C	198-202
8668123-9	1996	In fact, the kinetics of this copper loss closely parallels the kinetics of CUP1 and CRS5 gene repression.	Copper	30-36	metallothionein CUP1	Saccharomyces cerevisiae S288C	76-80
8628314-13	1996	This process may coordinate with the Cup1 pathway at different copper concentrations to prevent copper-induced toxicity.	Copper	63-69	metallothionein CUP1	Saccharomyces cerevisiae S288C	37-41
8628314-13	1996	This process may coordinate with the Cup1 pathway at different copper concentrations to prevent copper-induced toxicity.	Copper	96-102	metallothionein CUP1	Saccharomyces cerevisiae S288C	37-41
8585324-4	1995	G1810 is identical to the ACE1 gene sequenced by Szczypka and Thiele (1989), required for copper-inducible transcription of the CUP1 gene.	Copper	90-96	metallothionein CUP1	Saccharomyces cerevisiae S288C	128-132
8598289-2	1996	We demonstrate that transcription of the Saccharomyces cerevisiae MT gene CUP1 is strongly activated by the superoxide anion generator menadione.	Superoxides	108-124	metallothionein CUP1	Saccharomyces cerevisiae S288C	74-78
8598289-2	1996	We demonstrate that transcription of the Saccharomyces cerevisiae MT gene CUP1 is strongly activated by the superoxide anion generator menadione.	Vitamin K 3	135-144	metallothionein CUP1	Saccharomyces cerevisiae S288C	74-78
7766204-5	1995	The CUP1 is amplified to 3-14 copies with 2 kb-tandem-repeat units in the metal-resistant strains, whereas the wild-type strain contains only a single copy of the CUP1.	Metals	74-79	metallothionein CUP1	Saccharomyces cerevisiae S288C	4-8
7559639-1	1995	Cadmium-resistant Saccharomyces cerevisiae strain 301N exhibits high basal as well as cadmium-induced expression of the CUP1 metallothionein gene.	Cadmium	0-7	metallothionein CUP1	Saccharomyces cerevisiae S288C	120-124
7559639-1	1995	Cadmium-resistant Saccharomyces cerevisiae strain 301N exhibits high basal as well as cadmium-induced expression of the CUP1 metallothionein gene.	Cadmium	86-93	metallothionein CUP1	Saccharomyces cerevisiae S288C	120-124
7559639-2	1995	Since regulation of CUP1 is usually restricted to copper ions, our goal was to identify the factor responsible for the high metallothionein levels in strain 301N.	Copper	50-56	metallothionein CUP1	Saccharomyces cerevisiae S288C	20-24
8530401-1	1995	The copper toxicity of yeast lacking the CUP1 metallothionein is suppressed by overexpression of the CRS4 gene.	Copper	4-10	metallothionein CUP1	Saccharomyces cerevisiae S288C	41-45
7766204-6	1995	Although transcription of CUP1 is inducible by metals, the ACE1 protein serves a dual function as a sensor for copper and an inducer for CUP1 transcription in the copper-resistant strain.	Copper	163-169	metallothionein CUP1	Saccharomyces cerevisiae S288C	26-30
7766204-6	1995	Although transcription of CUP1 is inducible by metals, the ACE1 protein serves a dual function as a sensor for copper and an inducer for CUP1 transcription in the copper-resistant strain.	Copper	163-169	metallothionein CUP1	Saccharomyces cerevisiae S288C	137-141
7766204-7	1995	In the cadmium-resistant strain, the heat-shock factor having a point mutation may be the regulator for CUP1 transcription.	Cadmium	7-14	metallothionein CUP1	Saccharomyces cerevisiae S288C	104-108
7969120-1	1994	Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae.	Copper	57-63	metallothionein CUP1	Saccharomyces cerevisiae S288C	38-42
7762296-3	1995	(1) In the presence of copper salts, they were mitotically more stable than similarly designed control vectors lacking the CUP1 gene.	Copper	23-29	metallothionein CUP1	Saccharomyces cerevisiae S288C	123-127
7762296-5	1995	(2) Use of the CUP1-stabilized plasmids improved the production of hirudin by both copper-sensitive and copper-resistant hosts.	Copper	83-89	metallothionein CUP1	Saccharomyces cerevisiae S288C	15-19
7762296-5	1995	(2) Use of the CUP1-stabilized plasmids improved the production of hirudin by both copper-sensitive and copper-resistant hosts.	Copper	104-110	metallothionein CUP1	Saccharomyces cerevisiae S288C	15-19
7969120-1	1994	Yeast metallothionein, encoded by the CUP1 gene, and its copper-dependent transcriptional activator ACE1 play a key role in mediating copper resistance in Saccharomyces cerevisiae.	Copper	134-140	metallothionein CUP1	Saccharomyces cerevisiae S288C	38-42
7969120-2	1994	Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which permits yeast cells to grow at high concentrations of environmental copper, most notably when lactate is the sole carbon source.	Ethyl Methanesulfonate	9-31	metallothionein CUP1	Saccharomyces cerevisiae S288C	66-70
7969120-2	1994	Using an ethyl methanesulfonate mutant of a yeast strain in which CUP1 and ACE1 were deleted, we isolated a gene, designated CUP9, which permits yeast cells to grow at high concentrations of environmental copper, most notably when lactate is the sole carbon source.	Copper	205-211	metallothionein CUP1	Saccharomyces cerevisiae S288C	66-70
7969120-3	1994	Disruption of CUP9, which is located on chromosome XVI, caused a loss of copper resistance in strains which possessed CUP1 and ACE1, as well as in the cup1 ace1 deletion strain.	Copper	73-79	metallothionein CUP1	Saccharomyces cerevisiae S288C	118-122
8017099-6	1994	High activity of the CUP1 promoter was observed on both a fermentable (glucose) and a non-fermentable (ethanol) carbon source.	Glucose	71-78	metallothionein CUP1	Saccharomyces cerevisiae S288C	21-25
7969152-2	1994	In this report, we demonstrate that the Saccharomyces cerevisiae metallothionein gene, designated CUP1, is transcriptionally activated in response to heat shock and glucose starvation through the action of heat shock transcription factor (HSF) and a heat shock element located within the CUP1 promoter upstream regulatory region.	Glucose	165-172	metallothionein CUP1	Saccharomyces cerevisiae S288C	98-102
7969152-2	1994	In this report, we demonstrate that the Saccharomyces cerevisiae metallothionein gene, designated CUP1, is transcriptionally activated in response to heat shock and glucose starvation through the action of heat shock transcription factor (HSF) and a heat shock element located within the CUP1 promoter upstream regulatory region.	Glucose	165-172	metallothionein CUP1	Saccharomyces cerevisiae S288C	288-292
7969152-3	1994	CUP1 gene activation in response to both stresses occurs rapidly; however, heat shock activates CUP1 gene expression transiently, whereas glucose starvation activates CUP1 gene expression in a sustained manner for at least 2.5 h. Although a carboxyl-terminal HSF transcriptional activation domain is critical for the activation of CUP1 transcription in response to both heat shock stress and glucose starvation, this region is dispensable for transient heat shock activation of at least two genes encoding members of the S. cerevisiae hsp70 family.	Glucose	138-145	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
7969152-3	1994	CUP1 gene activation in response to both stresses occurs rapidly; however, heat shock activates CUP1 gene expression transiently, whereas glucose starvation activates CUP1 gene expression in a sustained manner for at least 2.5 h. Although a carboxyl-terminal HSF transcriptional activation domain is critical for the activation of CUP1 transcription in response to both heat shock stress and glucose starvation, this region is dispensable for transient heat shock activation of at least two genes encoding members of the S. cerevisiae hsp70 family.	Glucose	392-399	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
7969152-4	1994	Furthermore, inactivation of the chromosomal SNF1 gene, encoding a serine-threonine protein kinase, or the SNF4 gene, encoding a SNF1 cofactor, abolishes CUP1 transcriptional activation in response to glucose starvation without altering heat shock-induced transcription.	Glucose	201-208	metallothionein CUP1	Saccharomyces cerevisiae S288C	154-158
7929222-1	1994	Protection from copper toxicity in the bakers" yeast Saccharomyces cerevisiae involves the action of a copper binding metallothionein encoded by the CUP1 locus.	Copper	16-22	metallothionein CUP1	Saccharomyces cerevisiae S288C	149-153
7929222-1	1994	Protection from copper toxicity in the bakers" yeast Saccharomyces cerevisiae involves the action of a copper binding metallothionein encoded by the CUP1 locus.	Copper	103-109	metallothionein CUP1	Saccharomyces cerevisiae S288C	149-153
7929270-8	1994	A copper-dependent reporter gene construct, CUP1-lacZ, is not expressed in CTR1 mutants to the same level as in wild-type strains, and Cu,Zn superoxide dismutase activity is deficient in these mutants.	Copper	2-8	metallothionein CUP1	Saccharomyces cerevisiae S288C	44-48
8063718-3	1994	Constitutive expression of the Saccharomyces cerevisiae MT (CUP1) gene inhibited the accumulation of metal-phytochelatin complexes in both C. glabrata and S. pombe.	Metals	101-106	metallothionein CUP1	Saccharomyces cerevisiae S288C	60-64
8063718-9	1994	Likewise, metal exposed cultures of S. cerevisiae (cup1) transformed with C. glabrata MTII under the constitutive ADH1 promoter resulted in constitutive expression of MTII and accumulation of CuMTII complexes but no CdMTII complexes.	Metals	10-15	metallothionein CUP1	Saccharomyces cerevisiae S288C	51-55
8061521-4	1994	When expressed in an MT-deficient (cup1 delta) mutant of yeast, both MT1 and MT2 complemented the cup1 delta mutation, providing a high level of resistance to CuSO4 and moderate resistance to CdSO4.	Copper Sulfate	159-164	metallothionein CUP1	Saccharomyces cerevisiae S288C	35-39
8061521-4	1994	When expressed in an MT-deficient (cup1 delta) mutant of yeast, both MT1 and MT2 complemented the cup1 delta mutation, providing a high level of resistance to CuSO4 and moderate resistance to CdSO4.	Copper Sulfate	159-164	metallothionein CUP1	Saccharomyces cerevisiae S288C	98-102
8061521-4	1994	When expressed in an MT-deficient (cup1 delta) mutant of yeast, both MT1 and MT2 complemented the cup1 delta mutation, providing a high level of resistance to CuSO4 and moderate resistance to CdSO4.	cadmium sulfate	192-197	metallothionein CUP1	Saccharomyces cerevisiae S288C	35-39
8017099-6	1994	High activity of the CUP1 promoter was observed on both a fermentable (glucose) and a non-fermentable (ethanol) carbon source.	Ethanol	103-110	metallothionein CUP1	Saccharomyces cerevisiae S288C	21-25
8017099-6	1994	High activity of the CUP1 promoter was observed on both a fermentable (glucose) and a non-fermentable (ethanol) carbon source.	Carbon	112-118	metallothionein CUP1	Saccharomyces cerevisiae S288C	21-25
1516810-3	1992	However, assays of CUP1-specific mRNA revealed that the transcription of the CUP1 gene in the cadmium-resistant strain is constitutive and the rate of transcription is further increased by exposure to cadmium or copper ions.	Cadmium	94-101	metallothionein CUP1	Saccharomyces cerevisiae S288C	77-81
8293975-3	1993	The rrm3 mutation stimulates mitotic recombination in the naturally occurring tandem repeats of the rDNA and copper chelatin (CUP1) genes.	Copper	109-115	metallothionein CUP1	Saccharomyces cerevisiae S288C	126-130
8462846-3	1993	CUP1 is a nonessential gene that allows cells to grow in the presence of copper in a dosage-dependent manner.	Copper	73-79	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
1516810-3	1992	However, assays of CUP1-specific mRNA revealed that the transcription of the CUP1 gene in the cadmium-resistant strain is constitutive and the rate of transcription is further increased by exposure to cadmium or copper ions.	Cadmium	201-208	metallothionein CUP1	Saccharomyces cerevisiae S288C	77-81
1516810-3	1992	However, assays of CUP1-specific mRNA revealed that the transcription of the CUP1 gene in the cadmium-resistant strain is constitutive and the rate of transcription is further increased by exposure to cadmium or copper ions.	Copper	212-218	metallothionein CUP1	Saccharomyces cerevisiae S288C	77-81
1516810-4	1992	This result was confirmed by the appearance of constitutive-expression segregants from diploid crosses between the cadmium-resistant strain and a strain with a reporter gene having the promoter of CUP1.	Cadmium	115-122	metallothionein CUP1	Saccharomyces cerevisiae S288C	197-201
1916270-5	1991	The introduction of CUP1 cassettes allows these plasmids to direct Cu(2+)-regulated production of foreign proteins in yeast.	cupric ion	67-73	metallothionein CUP1	Saccharomyces cerevisiae S288C	20-24
1525854-0	1992	The gene for cadmium metallothionein from a cadmium-resistant yeast appears to be identical to CUP1 in a copper-resistant strain.	Cadmium	13-20	metallothionein CUP1	Saccharomyces cerevisiae S288C	95-99
1525854-0	1992	The gene for cadmium metallothionein from a cadmium-resistant yeast appears to be identical to CUP1 in a copper-resistant strain.	Copper	105-111	metallothionein CUP1	Saccharomyces cerevisiae S288C	95-99
1525854-1	1992	A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein with the same characteristics as the copper metallothionein that is encoded by CUP1 in a copper-resistant strain.	Cadmium	2-9	metallothionein CUP1	Saccharomyces cerevisiae S288C	169-173
1525854-1	1992	A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein with the same characteristics as the copper metallothionein that is encoded by CUP1 in a copper-resistant strain.	Copper	127-133	metallothionein CUP1	Saccharomyces cerevisiae S288C	169-173
1525854-2	1992	The structural gene for metallothionein from the cadmium-resistant strain resembles CUP1 in terms of the fragmentation patterns generated by restriction enzymes.	Cadmium	49-56	metallothionein CUP1	Saccharomyces cerevisiae S288C	84-88
1525854-5	1992	It appears that the same metallothionein gene, CUP1, is amplified in both cadmium- and copper-resistant yeasts.	Cadmium	74-81	metallothionein CUP1	Saccharomyces cerevisiae S288C	47-51
1525854-5	1992	It appears that the same metallothionein gene, CUP1, is amplified in both cadmium- and copper-resistant yeasts.	Copper	87-93	metallothionein CUP1	Saccharomyces cerevisiae S288C	47-51
1924315-3	1991	We show that the ACE1 transcriptional activator protein, which is responsible for the induction of yeast metallothionein (CUP1) in response to copper, also controls the SOD1 response to copper.	Copper	186-192	metallothionein CUP1	Saccharomyces cerevisiae S288C	122-126
1516810-1	1992	A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein encoded by the CUP1 gene as does a copper-resistant strain.	Cadmium	2-9	metallothionein CUP1	Saccharomyces cerevisiae S288C	105-109
1516810-1	1992	A cadmium-resistant strain of Saccharomyces cerevisiae produces a cadmium metallothionein encoded by the CUP1 gene as does a copper-resistant strain.	Copper	125-131	metallothionein CUP1	Saccharomyces cerevisiae S288C	105-109
1516810-3	1992	However, assays of CUP1-specific mRNA revealed that the transcription of the CUP1 gene in the cadmium-resistant strain is constitutive and the rate of transcription is further increased by exposure to cadmium or copper ions.	Cadmium	94-101	metallothionein CUP1	Saccharomyces cerevisiae S288C	19-23
1996089-1	1991	In the yeast Saccharomyces cerevisiae, transcription of the metallothionein gene CUP1 is induced by copper and silver.	Copper	100-106	metallothionein CUP1	Saccharomyces cerevisiae S288C	81-85
1924315-3	1991	We show that the ACE1 transcriptional activator protein, which is responsible for the induction of yeast metallothionein (CUP1) in response to copper, also controls the SOD1 response to copper.	Copper	143-149	metallothionein CUP1	Saccharomyces cerevisiae S288C	122-126
2017134-0	1991	Multicopy CUP1 plasmids enhance cadmium and copper resistance levels in yeast.	Cadmium	32-39	metallothionein CUP1	Saccharomyces cerevisiae S288C	10-14
2017134-0	1991	Multicopy CUP1 plasmids enhance cadmium and copper resistance levels in yeast.	Copper	44-50	metallothionein CUP1	Saccharomyces cerevisiae S288C	10-14
2017134-3	1991	Northern analysis showed that addition of cadmium (0.02 microM) or copper (50 microM) to overnight liquid cultures of yeast induced expression of CUP1 transcripts from both chromosomal and plasmid-borne gene copies.	Cadmium	42-49	metallothionein CUP1	Saccharomyces cerevisiae S288C	146-150
2017134-3	1991	Northern analysis showed that addition of cadmium (0.02 microM) or copper (50 microM) to overnight liquid cultures of yeast induced expression of CUP1 transcripts from both chromosomal and plasmid-borne gene copies.	Copper	67-73	metallothionein CUP1	Saccharomyces cerevisiae S288C	146-150
1996089-1	1991	In the yeast Saccharomyces cerevisiae, transcription of the metallothionein gene CUP1 is induced by copper and silver.	Silver	111-117	metallothionein CUP1	Saccharomyces cerevisiae S288C	81-85
2017134-5	1991	Thus, CUP1 gene expression in yeast appears to be influenced differently by cadmium and copper ions.	Cadmium	76-83	metallothionein CUP1	Saccharomyces cerevisiae S288C	6-10
2017134-5	1991	Thus, CUP1 gene expression in yeast appears to be influenced differently by cadmium and copper ions.	Copper	88-94	metallothionein CUP1	Saccharomyces cerevisiae S288C	6-10
1996089-2	1991	Strains with a complete deletion of the ACE1 gene, the copper-dependent activator of CUP1 transcription, are hypersensitive to copper.	Copper	55-61	metallothionein CUP1	Saccharomyces cerevisiae S288C	85-89
2017134-6	1991	Resistance to heavy metal poisoning resulted from enhanced gene product levels attributable to amplification of the CUP1 gene as well as to increased transcriptions.	Metals	20-25	metallothionein CUP1	Saccharomyces cerevisiae S288C	116-120
1996089-2	1991	Strains with a complete deletion of the ACE1 gene, the copper-dependent activator of CUP1 transcription, are hypersensitive to copper.	Copper	127-133	metallothionein CUP1	Saccharomyces cerevisiae S288C	85-89
2167439-1	1990	CUP2 is a copper-dependent transcriptional activator of the yeast CUP1 metallothionein gene.	Copper	10-16	metallothionein CUP1	Saccharomyces cerevisiae S288C	66-70
1648291-3	1991	Resistance to these latter metal ions, in the absence of induction by copper, suggested that the CUP1 gene is constitutively expressed in the foreign background.	Metals	27-32	metallothionein CUP1	Saccharomyces cerevisiae S288C	97-101
1648291-4	1991	Introduction of the lacZ reporter gene from Escherichia coli into a cloning site downstream from the CUP1 promoter showed that expression of this gene is constitutive in K. lactis but in S. cerevisiae induction by copper is necessary.	Copper	214-220	metallothionein CUP1	Saccharomyces cerevisiae S288C	101-105
1648291-6	1991	It is suggested that a K. lactis protein, normally involved in activating transcription of the resident CUP1 gene in the presence of copper, can promote transcription in the absence of metal ion by binding to the upstream activation sequence of the introduced CUP1 gene.	Copper	133-139	metallothionein CUP1	Saccharomyces cerevisiae S288C	104-108
1648291-6	1991	It is suggested that a K. lactis protein, normally involved in activating transcription of the resident CUP1 gene in the presence of copper, can promote transcription in the absence of metal ion by binding to the upstream activation sequence of the introduced CUP1 gene.	Copper	133-139	metallothionein CUP1	Saccharomyces cerevisiae S288C	260-264
1648291-6	1991	It is suggested that a K. lactis protein, normally involved in activating transcription of the resident CUP1 gene in the presence of copper, can promote transcription in the absence of metal ion by binding to the upstream activation sequence of the introduced CUP1 gene.	Metals	185-190	metallothionein CUP1	Saccharomyces cerevisiae S288C	104-108
1648291-6	1991	It is suggested that a K. lactis protein, normally involved in activating transcription of the resident CUP1 gene in the presence of copper, can promote transcription in the absence of metal ion by binding to the upstream activation sequence of the introduced CUP1 gene.	Metals	185-190	metallothionein CUP1	Saccharomyces cerevisiae S288C	260-264
1986241-1	1991	Transcription of the Saccharomyces cerevisiae metallothionein gene CUP1 is induced in response to high environmental levels of copper.	Copper	127-133	metallothionein CUP1	Saccharomyces cerevisiae S288C	67-71
1986241-3	1991	In this study, we found that deleting the entire coding sequence of the ACE1 gene resulted in a decrease in basal-level transcription of CUP1 to low but detectable levels and conferred a copper-sensitive phenotype to the cells.	Copper	187-193	metallothionein CUP1	Saccharomyces cerevisiae S288C	137-141
1714451-11	1991	Although this gene is unresponsive to silver ions in vivo, in contrast to the yeast copper-responsive CUP1 gene (Furst, P., Hu, S., Hackett, R., and Hamer, D. (1988) Cell 55, 705-717), it does respond to mercury ions, albeit with less sensitivity.	Copper	84-90	metallothionein CUP1	Saccharomyces cerevisiae S288C	102-106
1714451-11	1991	Although this gene is unresponsive to silver ions in vivo, in contrast to the yeast copper-responsive CUP1 gene (Furst, P., Hu, S., Hackett, R., and Hamer, D. (1988) Cell 55, 705-717), it does respond to mercury ions, albeit with less sensitivity.	Mercury	204-211	metallothionein CUP1	Saccharomyces cerevisiae S288C	102-106
2174892-2	1990	We have cloned a cDNA encoding the human vitamin D receptor (VDR) into a high copy yeast plasmid controlled transcriptionally by the copper-inducible metallothionein (CUP-1) promoter to produce YEpV1.	Copper	133-139	metallothionein CUP1	Saccharomyces cerevisiae S288C	167-172
2167439-2	1990	In the presence of Cu+ and Ag+) ions its DNA-binding domain is thought to fold as a cysteine-coordinated Cu cluster which recognizes the palindromic CUP1 upstream activation sequence (UASc).	Copper	19-22	metallothionein CUP1	Saccharomyces cerevisiae S288C	149-153
2167439-2	1990	In the presence of Cu+ and Ag+) ions its DNA-binding domain is thought to fold as a cysteine-coordinated Cu cluster which recognizes the palindromic CUP1 upstream activation sequence (UASc).	Cysteine	84-92	metallothionein CUP1	Saccharomyces cerevisiae S288C	149-153
2167439-2	1990	In the presence of Cu+ and Ag+) ions its DNA-binding domain is thought to fold as a cysteine-coordinated Cu cluster which recognizes the palindromic CUP1 upstream activation sequence (UASc).	Copper	19-21	metallothionein CUP1	Saccharomyces cerevisiae S288C	149-153
2117558-3	1990	The Mto and Mtn cDNAs, when placed under the control of CUP1 or PGK promoters, can confer a copper-resistance phenotype to copper-hypersensitive cells.	Copper	92-98	metallothionein CUP1	Saccharomyces cerevisiae S288C	56-60
2117558-3	1990	The Mto and Mtn cDNAs, when placed under the control of CUP1 or PGK promoters, can confer a copper-resistance phenotype to copper-hypersensitive cells.	Copper	123-129	metallothionein CUP1	Saccharomyces cerevisiae S288C	56-60
2188974-3	1990	A wild type copper-resistant CUP 1R-containing strain and a strain carrying a deletion of the CUP1 locus (yeast copper metallothionein) exhibited quantitatively similar saturable energy-dependent 64Cu2+ uptake when cultures were pregrown in copper-free media (medium [Cu] approximately 15 nM).	Copper	112-118	metallothionein CUP1	Saccharomyces cerevisiae S288C	94-98
2188656-0	1990	Expression of alcohol-inducible rabbit liver cytochrome P-450 3a (P-450IIE1) in Saccharomyces cerevisiae with the copper-inducible CUP1 promoter.	Alcohols	14-21	metallothionein CUP1	Saccharomyces cerevisiae S288C	131-135
2188656-0	1990	Expression of alcohol-inducible rabbit liver cytochrome P-450 3a (P-450IIE1) in Saccharomyces cerevisiae with the copper-inducible CUP1 promoter.	Copper	114-120	metallothionein CUP1	Saccharomyces cerevisiae S288C	131-135
2188656-1	1990	The expression of the cDNA for alcohol-inducible rabbit liver microsomal cytochrome P-450 form 3a (P450IIE1) in Saccharomyces cerevisiae, with the use of the copper-inducible yeast metallothionein (CUP1) promoter and the ADH1 promoter, is described.	Alcohols	31-38	metallothionein CUP1	Saccharomyces cerevisiae S288C	198-202
2188656-1	1990	The expression of the cDNA for alcohol-inducible rabbit liver microsomal cytochrome P-450 form 3a (P450IIE1) in Saccharomyces cerevisiae, with the use of the copper-inducible yeast metallothionein (CUP1) promoter and the ADH1 promoter, is described.	Copper	158-164	metallothionein CUP1	Saccharomyces cerevisiae S288C	198-202
2188974-3	1990	A wild type copper-resistant CUP 1R-containing strain and a strain carrying a deletion of the CUP1 locus (yeast copper metallothionein) exhibited quantitatively similar saturable energy-dependent 64Cu2+ uptake when cultures were pregrown in copper-free media (medium [Cu] approximately 15 nM).	Copper	12-18	metallothionein CUP1	Saccharomyces cerevisiae S288C	94-98
2500675-4	1989	In copper sensitive yeast, multiple copies of the CUP1 cassettes confer copper resistance due to the production of the copper metallothionein.	Copper	3-9	metallothionein CUP1	Saccharomyces cerevisiae S288C	50-54
2188974-3	1990	A wild type copper-resistant CUP 1R-containing strain and a strain carrying a deletion of the CUP1 locus (yeast copper metallothionein) exhibited quantitatively similar saturable energy-dependent 64Cu2+ uptake when cultures were pregrown in copper-free media (medium [Cu] approximately 15 nM).	Copper	112-118	metallothionein CUP1	Saccharomyces cerevisiae S288C	94-98
2188974-3	1990	A wild type copper-resistant CUP 1R-containing strain and a strain carrying a deletion of the CUP1 locus (yeast copper metallothionein) exhibited quantitatively similar saturable energy-dependent 64Cu2+ uptake when cultures were pregrown in copper-free media (medium [Cu] approximately 15 nM).	64cu2+	196-202	metallothionein CUP1	Saccharomyces cerevisiae S288C	94-98
35018465-2	2022	When and how adaptive mutations form is often hard to discern, but we have shown that adaptive copy number amplification of the copper resistance gene CUP1 occurs in response to environmental copper due to CUP1 transcriptional activation.	Copper	128-134	metallothionein CUP1	Saccharomyces cerevisiae S288C	151-155
35018465-2	2022	When and how adaptive mutations form is often hard to discern, but we have shown that adaptive copy number amplification of the copper resistance gene CUP1 occurs in response to environmental copper due to CUP1 transcriptional activation.	Copper	128-134	metallothionein CUP1	Saccharomyces cerevisiae S288C	206-210
35018465-2	2022	When and how adaptive mutations form is often hard to discern, but we have shown that adaptive copy number amplification of the copper resistance gene CUP1 occurs in response to environmental copper due to CUP1 transcriptional activation.	Copper	192-198	metallothionein CUP1	Saccharomyces cerevisiae S288C	151-155
35018465-2	2022	When and how adaptive mutations form is often hard to discern, but we have shown that adaptive copy number amplification of the copper resistance gene CUP1 occurs in response to environmental copper due to CUP1 transcriptional activation.	Copper	192-198	metallothionein CUP1	Saccharomyces cerevisiae S288C	206-210
35018465-7	2022	CUP1 amplification is also critically dependent on late-firing replication origins present in the CUP1 repeats, and mutations that remove or inactivate these origins strongly suppress the acquisition of copper resistance.	Copper	203-209	metallothionein CUP1	Saccharomyces cerevisiae S288C	0-4
35018465-7	2022	CUP1 amplification is also critically dependent on late-firing replication origins present in the CUP1 repeats, and mutations that remove or inactivate these origins strongly suppress the acquisition of copper resistance.	Copper	203-209	metallothionein CUP1	Saccharomyces cerevisiae S288C	98-102
34874147-5	2021	As compared to the native Cu2+-inducible CUP1 promoter, the CuIGR4 system amplified the response to copper by as much as 2.7 folds, resulting in 72-fold induction of EGFP expression and a 33-fold change in lycopene production (3-100 mg/L) with addition of 20 muM copper.	cupric ion	26-30	metallothionein CUP1	Saccharomyces cerevisiae S288C	41-45
34874147-5	2021	As compared to the native Cu2+-inducible CUP1 promoter, the CuIGR4 system amplified the response to copper by as much as 2.7 folds, resulting in 72-fold induction of EGFP expression and a 33-fold change in lycopene production (3-100 mg/L) with addition of 20 muM copper.	Copper	100-106	metallothionein CUP1	Saccharomyces cerevisiae S288C	41-45
34874147-5	2021	As compared to the native Cu2+-inducible CUP1 promoter, the CuIGR4 system amplified the response to copper by as much as 2.7 folds, resulting in 72-fold induction of EGFP expression and a 33-fold change in lycopene production (3-100 mg/L) with addition of 20 muM copper.	Lycopene	206-214	metallothionein CUP1	Saccharomyces cerevisiae S288C	41-45
34874147-5	2021	As compared to the native Cu2+-inducible CUP1 promoter, the CuIGR4 system amplified the response to copper by as much as 2.7 folds, resulting in 72-fold induction of EGFP expression and a 33-fold change in lycopene production (3-100 mg/L) with addition of 20 muM copper.	Copper	263-269	metallothionein CUP1	Saccharomyces cerevisiae S288C	41-45
34335494-6	2021	Seven targets were identified, including CAD1 and CUP1 that are known to improve cadmium tolerance, as well as CRS5, NRG1, PPH21, BMH1, and QCR6 that are less studied.	Cadmium	81-88	metallothionein CUP1	Saccharomyces cerevisiae S288C	50-54
34335494-7	2021	In the wild-type strain, cadmium exposure activated gene transcription of CAD1, CRS5, CUP1, and NRG1 and repressed PPH21, as revealed by real-time quantitative PCR analyses.	Cadmium	25-32	metallothionein CUP1	Saccharomyces cerevisiae S288C	86-90
34335494-9	2021	Synergistic improvement in cadmium tolerance was observed with episomal co-expression of CRS5 and CUP1.	Cadmium	27-34	metallothionein CUP1	Saccharomyces cerevisiae S288C	98-102
2500675-4	1989	In copper sensitive yeast, multiple copies of the CUP1 cassettes confer copper resistance due to the production of the copper metallothionein.	Copper	72-78	metallothionein CUP1	Saccharomyces cerevisiae S288C	50-54
3043194-1	1988	Copper resistance in Saccharomyces cerevisiae is mediated, in large part, by the CUP1 locus, which encodes a low-molecular-weight, cysteine-rich metal-binding protein.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	81-85
2651899-1	1989	The ACE1 gene of the yeast Saccharomyces cerevisiae is required for copper-inducible transcription of the metallothionein gene (CUP1).	Copper	68-74	metallothionein CUP1	Saccharomyces cerevisiae S288C	128-132
2653812-1	1989	The yeast CUP1 gene codes for a copper-binding protein similar to metallothionein.	Copper	32-38	metallothionein CUP1	Saccharomyces cerevisiae S288C	10-14
2653812-2	1989	Copper sensitive cup1s strains contain a single copy of the CUP1 locus.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	17-21
2653812-2	1989	Copper sensitive cup1s strains contain a single copy of the CUP1 locus.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	60-64
2653812-4	1989	We isolated 12 ethyl methane sulfonate-induced copper sensitive mutants in a wild-type CUP1r parental strain, X2180-1A.	Ethyl Methanesulfonate	15-38	metallothionein CUP1	Saccharomyces cerevisiae S288C	87-91
2653812-4	1989	We isolated 12 ethyl methane sulfonate-induced copper sensitive mutants in a wild-type CUP1r parental strain, X2180-1A.	Copper	47-53	metallothionein CUP1	Saccharomyces cerevisiae S288C	87-91
2643107-1	1989	Copper-inducible transcription of the yeast metallothionein gene (CUP1) occurs by means of cis-acting upstream activation sequences (UAS) and trans-acting cellular factors.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	66-70
3043194-2	1988	Expression of the CUP1 gene is regulated at the level of transcriptional induction in response to high environmental copper levels.	Copper	117-123	metallothionein CUP1	Saccharomyces cerevisiae S288C	18-22
3043194-3	1988	This report describes the isolation of a yeast mutant, ace1-1, which is defective in the activation of CUP1 expression upon exposure to exogenous copper.	Copper	146-152	metallothionein CUP1	Saccharomyces cerevisiae S288C	103-107
3043194-5	1988	The wild-type ACE1 gene was isolated by in vivo complementation and restores copper inducibility of CUP1 expression and copper resistance to the otherwise copper-sensitive ace1-1 mutant.	Copper	77-83	metallothionein CUP1	Saccharomyces cerevisiae S288C	100-104
3043194-8	1988	The ACE1 gene appears to play a direct or indirect positive role in activation of CUP1 expression in response to elevated copper concentrations.	Copper	122-128	metallothionein CUP1	Saccharomyces cerevisiae S288C	82-86
3054502-7	1988	Chromosome nondisjunction in chl3 is also confirmed by the data on the mini-chromosome carrying CUP1 gene responsible for copper-resistance in yeast.	Copper	122-128	metallothionein CUP1	Saccharomyces cerevisiae S288C	96-100
3054502-9	1988	Elevated copper resistance of chl3 transformants is caused by the transit accumulation of CUP1-carrying mini-chromosome in part of the cell population as a result of segregation mistakes upon cell divisions.	Copper	9-15	metallothionein CUP1	Saccharomyces cerevisiae S288C	90-94
3283130-1	1988	Cu-metallothionein was purified from Saccharomyces cerevisiae harboring plasmids containing mutated CUP1 metallothionein genes resulting in deletions at the carboxy-terminal end of the polypeptide.	cu-metallothionein	0-18	metallothionein CUP1	Saccharomyces cerevisiae S288C	100-104
3275668-2	1988	By creating a series of truncation and amino acid substitutions in CUP1, we show that the ability of the protein to autoregulate is directly correlated to its ability to bind and detoxify copper.	Copper	188-194	metallothionein CUP1	Saccharomyces cerevisiae S288C	67-71
3057171-4	1988	Copper resistance (CUPr) is correlated with amplification of CUP1 and resulted in a higher copy number of this gene on chromosome VIII.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	61-65
2959531-1	1987	A functional copperthionein (CUP1) gene in Saccharomyces cerevisiae is essential to prevent copper-mediated cytotoxicity, but is dispensable for cell growth in the absence of exogenous copper.	Copper	13-19	metallothionein CUP1	Saccharomyces cerevisiae S288C	29-33
3275610-3	1988	Furthermore, E. coli cells expressing CUP1 acquired a new, inducible ability to selectively sequester heavy metal ions from the growth medium.	Metals	108-113	metallothionein CUP1	Saccharomyces cerevisiae S288C	38-42
2959531-1	1987	A functional copperthionein (CUP1) gene in Saccharomyces cerevisiae is essential to prevent copper-mediated cytotoxicity, but is dispensable for cell growth in the absence of exogenous copper.	Copper	92-98	metallothionein CUP1	Saccharomyces cerevisiae S288C	29-33
2959531-4	1987	The expression of the CUP1 gene is induced at the level of transcription by copper via cis-dominant upstream control sequences which are tandemly repeated.	Copper	76-82	metallothionein CUP1	Saccharomyces cerevisiae S288C	22-26
2959531-5	1987	Synthetic CUP1 upstream control sequences confer copper inducibility on a heterologous yeast promoter in a manner similar to that observed for the authentic upstream control region.	Copper	49-55	metallothionein CUP1	Saccharomyces cerevisiae S288C	10-14
3536930-4	1986	Metal resistance of the cup1 delta strain with and without the metallothionein-expressing vectors was analyzed.	Metals	0-5	metallothionein CUP1	Saccharomyces cerevisiae S288C	24-28
3536930-6	1986	Two mutant versions of the metallothionein gene were constructed and tested for their ability to confer metal resistance in the cup1 delta background.	Metals	27-32	metallothionein CUP1	Saccharomyces cerevisiae S288C	128-132
3536930-9	1986	Expression of these genes under the CUP1 promoter provided significant protection from copper, but none of the other metals tested.	Copper	87-93	metallothionein CUP1	Saccharomyces cerevisiae S288C	36-40
3887570-1	1985	The CUP1 gene of yeast encodes a small, metallothionein-like protein that binds to and is inducible by copper.	Copper	103-109	metallothionein CUP1	Saccharomyces cerevisiae S288C	4-8
3537699-1	1986	Transcription of the Saccharomyces cerevisiae copper-metallothionein gene, CUP1, inducible by copper.	Copper	46-52	metallothionein CUP1	Saccharomyces cerevisiae S288C	75-79
3549458-5	1986	In contrast, in a strain deleted for the chromosomal copy of CUP1, synthesis of GalK is constitutive but can be induced to yet higher levels by copper.	Copper	144-150	metallothionein CUP1	Saccharomyces cerevisiae S288C	61-65
3549458-9	1986	The possible roles of metallothionein and free copper in CUP1 regulation are discussed.	Copper	47-53	metallothionein CUP1	Saccharomyces cerevisiae S288C	57-61
6621529-1	1983	The gene copy number at the CUP1 locus and the resistance level to external copper was directly correlated in five wild-type commercial Saccharomyces strains.	Copper	76-82	metallothionein CUP1	Saccharomyces cerevisiae S288C	28-32
6364141-1	1984	Copper resistance in yeast is controlled by the CUP1 locus.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	48-52
24173415-0	1983	Gene amplification in yeast: CUP1 copy number regulates copper resistance.	Copper	56-62	metallothionein CUP1	Saccharomyces cerevisiae S288C	29-33
6621529-2	1983	An increased copy number of the CUP1 gene leads to increased accumulation of chelatin mRNA, which codes for a low-molecular-weight, copper-binding protein.	Copper	132-138	metallothionein CUP1	Saccharomyces cerevisiae S288C	32-36
33729510-3	2021	To study copy number variation at tandem repeats, we used two well-studied repetitive arrays in the budding yeast genome, the ribosomal DNA (rDNA) locus, and the copper-inducible CUP1 gene array.	Copper	162-168	metallothionein CUP1	Saccharomyces cerevisiae S288C	179-183
33580598-2	2021	Hence, the copper-inducible CUP1 gene promoter from the related yeast Saccharomyces cerevisiae was used to express human gelatin.	Copper	11-17	metallothionein CUP1	Saccharomyces cerevisiae S288C	28-32
33580598-3	2021	Multimerization of a potential copper response element in the CUP1 promoter, a S. cerevisiae Ace1p binding site, significantly increased gelatin expression.	Copper	31-37	metallothionein CUP1	Saccharomyces cerevisiae S288C	62-66
33257505-3	2021	AceI responds to toxic copper levels by transcriptional regulation of detoxification genes CUP1 and CRS5 Cup1 metallothionein (MT) confers protection against toxic copper levels.	acei	0-4	metallothionein CUP1	Saccharomyces cerevisiae S288C	91-95
33398345-4	2021	When targeted to the CUP1 array comprising ~16 repeat units, dCas9 induced its contraction in most cells, especially in the presence of nicotinamide.	dcas9	61-66	metallothionein CUP1	Saccharomyces cerevisiae S288C	21-25
33398345-4	2021	When targeted to the CUP1 array comprising ~16 repeat units, dCas9 induced its contraction in most cells, especially in the presence of nicotinamide.	Niacinamide	136-148	metallothionein CUP1	Saccharomyces cerevisiae S288C	21-25
33498600-5	2021	Furthermore, inspired by the differential gene expression analysis which indicated that ADY2, HES1, and CUP1 showed the most remarkable changes, we found that the improvement of carotenoid accumulation in YPM media was mainly due to the copper ions, since supplementation of 0.08 mM CuSO4 in YPD media could increase carotenoid yield 9.2-fold.	Carotenoids	178-188	metallothionein CUP1	Saccharomyces cerevisiae S288C	104-108
33257505-3	2021	AceI responds to toxic copper levels by transcriptional regulation of detoxification genes CUP1 and CRS5 Cup1 metallothionein (MT) confers protection against toxic copper levels.	Copper	23-29	metallothionein CUP1	Saccharomyces cerevisiae S288C	91-95
33257505-3	2021	AceI responds to toxic copper levels by transcriptional regulation of detoxification genes CUP1 and CRS5 Cup1 metallothionein (MT) confers protection against toxic copper levels.	Copper	164-170	metallothionein CUP1	Saccharomyces cerevisiae S288C	91-95
32996528-5	2020	Two MTs, CUP1 and Crs5, originating from Saccharomyces cerevisiae predominantly bind to copper though are capable of binding with zinc and cadmium ions.	Copper	88-94	metallothionein CUP1	Saccharomyces cerevisiae S288C	9-13
32301942-10	2020	Observed higher-mass copper-detected peaks were tentatively assigned to copper-bound metallothioneins Cup1 and Crs5.	Copper	21-27	metallothionein CUP1	Saccharomyces cerevisiae S288C	102-106
32996528-5	2020	Two MTs, CUP1 and Crs5, originating from Saccharomyces cerevisiae predominantly bind to copper though are capable of binding with zinc and cadmium ions.	Cadmium	139-146	metallothionein CUP1	Saccharomyces cerevisiae S288C	9-13
32301942-10	2020	Observed higher-mass copper-detected peaks were tentatively assigned to copper-bound metallothioneins Cup1 and Crs5.	Copper	72-78	metallothionein CUP1	Saccharomyces cerevisiae S288C	102-106
32301942-11	2020	FTSs from strains in which Cup1 or the Cox17 copper chaperone were deleted altered the distribution of LMM copper complexes.	Copper	107-113	metallothionein CUP1	Saccharomyces cerevisiae S288C	27-31
32210477-7	2020	We also showed that cells experience increased sensitivity to copper toxicity when remodelers accumulate at the INO1 promoter due to the decreased CUP1 expression.	Copper	62-68	metallothionein CUP1	Saccharomyces cerevisiae S288C	147-151
32436567-3	2020	Copper tolerance is related to the copy number of the CUP1 gene, encoding for a metallothionein involved in copper detoxification.	Copper	0-6	metallothionein CUP1	Saccharomyces cerevisiae S288C	54-58
32436567-3	2020	Copper tolerance is related to the copy number of the CUP1 gene, encoding for a metallothionein involved in copper detoxification.	Copper	108-114	metallothionein CUP1	Saccharomyces cerevisiae S288C	54-58
31037485-12	2019	The PpMTs, except PpMT1.2b, were also able to confer metal tolerance and accumulation when heterologously expressed in the  cup1 yeast.	Metals	53-58	metallothionein CUP1	Saccharomyces cerevisiae S288C	124-128
31794573-2	2019	Here, we demonstrate that yeast aged under environmental copper accumulate high levels of eccDNA containing the copper-resistance gene CUP1.	Copper	57-63	metallothionein CUP1	Saccharomyces cerevisiae S288C	135-139
31794573-2	2019	Here, we demonstrate that yeast aged under environmental copper accumulate high levels of eccDNA containing the copper-resistance gene CUP1.	Copper	112-118	metallothionein CUP1	Saccharomyces cerevisiae S288C	135-139
31794573-4	2019	We have developed a sensitive and quantitative eccDNA sequencing pipeline that reveals CUP1 eccDNA accumulation on copper exposure to be exquisitely site specific, with no other detectable changes across the eccDNA complement.	Copper	115-121	metallothionein CUP1	Saccharomyces cerevisiae S288C	87-91
29098364-4	2018	Correlation between altered tRNA and protein level was shown by survival of copper sensitive S. cerevisiae cells in the presence of copper ions by an increased transcription of tRNAArgCCG molecules, recognizing rare codons in a modified CUP1 gene.	Copper	76-82	metallothionein CUP1	Saccharomyces cerevisiae S288C	237-241
30850698-6	2019	Based on these findings, we improved isobutanol production by expressing alsS under the control of a copper-inducible CUP1 promoter, and by increasing translational efficiency of the ILV5DeltaN48 and ILV3DeltaN19 genes by adding Kozak sequence.	isobutyl alcohol	37-47	metallothionein CUP1	Saccharomyces cerevisiae S288C	118-122
30850698-6	2019	Based on these findings, we improved isobutanol production by expressing alsS under the control of a copper-inducible CUP1 promoter, and by increasing translational efficiency of the ILV5DeltaN48 and ILV3DeltaN19 genes by adding Kozak sequence.	Copper	101-107	metallothionein CUP1	Saccharomyces cerevisiae S288C	118-122
29098364-4	2018	Correlation between altered tRNA and protein level was shown by survival of copper sensitive S. cerevisiae cells in the presence of copper ions by an increased transcription of tRNAArgCCG molecules, recognizing rare codons in a modified CUP1 gene.	Copper	132-138	metallothionein CUP1	Saccharomyces cerevisiae S288C	237-241
28381587-6	2017	Lastly, we showed that high levels of copper (previously shown to elevate CUP1 transcription) lead to a substantial elevation in rate of both interhomolog and intra/sister chromatid recombination in the CUP1 array; recombination events that delete the URA3 insertion from the CUP1 array occur at a rate of >10-3/division in unselected cells.	Copper	38-44	metallothionein CUP1	Saccharomyces cerevisiae S288C	74-78
28869270-6	2017	Genes responsible for genetic variation in response to copper were mapped to the ZRT2 and the CUP1 loci.	Copper	55-61	metallothionein CUP1	Saccharomyces cerevisiae S288C	94-98
28381587-6	2017	Lastly, we showed that high levels of copper (previously shown to elevate CUP1 transcription) lead to a substantial elevation in rate of both interhomolog and intra/sister chromatid recombination in the CUP1 array; recombination events that delete the URA3 insertion from the CUP1 array occur at a rate of >10-3/division in unselected cells.	Copper	38-44	metallothionein CUP1	Saccharomyces cerevisiae S288C	203-207
28381587-6	2017	Lastly, we showed that high levels of copper (previously shown to elevate CUP1 transcription) lead to a substantial elevation in rate of both interhomolog and intra/sister chromatid recombination in the CUP1 array; recombination events that delete the URA3 insertion from the CUP1 array occur at a rate of >10-3/division in unselected cells.	Copper	38-44	metallothionein CUP1	Saccharomyces cerevisiae S288C	203-207