PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 31164445-2 2019 Lag1 and Lac1 lie in an enzymatic branch point of the sphingolipid pathway that is interconnected by the activity of the C4 hydroxylase, Sur2. Sphingolipids 54-66 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 0-4 31164445-3 2019 By uncoupling the enzymatic branch point and using lipidomic mass spectrometry, metabolic labeling and in vitro assays we show that Lag1 preferentially synthesizes phyto-sphingolipids. phyto-sphingolipids 164-183 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 132-136 31164445-4 2019 Using photo-bleaching experiments we show that Lag1 is uniquely required for the establishment of a lateral diffusion barrier in the nuclear envelope, which depends on phytoceramide. phytoceramide 168-181 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 47-51 23472095-9 2013 BarA is a homologue of the yeast Lag1 acyl-CoA-dependent ceramide synthase, which catalyzes the condensation of phytosphingosine with a fatty acyl-CoA to form phytoceramide. phytosphingosine 112-128 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 33-37 26752183-1 2016 Ceramide is synthesized in yeast by two redundant acyl-CoA dependent synthases, Lag1 and Lac1. Ceramides 0-8 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 80-84 26752183-2 2016 In lag1 lac1 cells, free fatty acids and sphingoid bases are elevated, and ceramides are produced through the redundant alkaline ceramidases Ypc1 and Ydc1, working backwards. Fatty Acids, Nonesterified 22-38 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 3-7 26752183-2 2016 In lag1 lac1 cells, free fatty acids and sphingoid bases are elevated, and ceramides are produced through the redundant alkaline ceramidases Ypc1 and Ydc1, working backwards. Sphingosine 43-52 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 3-7 26752183-2 2016 In lag1 lac1 cells, free fatty acids and sphingoid bases are elevated, and ceramides are produced through the redundant alkaline ceramidases Ypc1 and Ydc1, working backwards. Ceramides 77-86 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 3-7 26752183-4 2016 Here we show that these residual sphingolipids are not synthesized by YPR114w or YJR116w, proteins of unknown function showing a high degree of homology to Lag1 and Lac1. Sphingolipids 33-46 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 156-160 26752183-5 2016 Indeed, the hextuple lag1 lac1 ypc1 ydc1 ypr114w yjr116w mutant still contains ceramides and complex sphingolipids. Ceramides 85-94 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 21-43 26752183-5 2016 Indeed, the hextuple lag1 lac1 ypc1 ydc1 ypr114w yjr116w mutant still contains ceramides and complex sphingolipids. Sphingolipids 107-120 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 21-43 23472095-9 2013 BarA is a homologue of the yeast Lag1 acyl-CoA-dependent ceramide synthase, which catalyzes the condensation of phytosphingosine with a fatty acyl-CoA to form phytoceramide. Acyl Coenzyme A 38-46 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 33-37 23472095-9 2013 BarA is a homologue of the yeast Lag1 acyl-CoA-dependent ceramide synthase, which catalyzes the condensation of phytosphingosine with a fatty acyl-CoA to form phytoceramide. phytoceramide 159-172 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 33-37 23226203-7 2012 These results suggest that ceramide production contributes to cell death induced by acetic acid, especially through hydrolysis of complex sphingolipids catalyzed by Isc1p and de novo synthesis catalyzed by Lag1p, and provide the first in vivo indication of its involvement in mitochondrial outer membrane permeabilization in yeast. Ceramides 27-35 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 206-211 22211636-1 2012 Lag1p and Lac1p catalyse ceramide synthesis in Saccharomyces cerevisiae. Ceramides 25-33 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 0-5 23226203-7 2012 These results suggest that ceramide production contributes to cell death induced by acetic acid, especially through hydrolysis of complex sphingolipids catalyzed by Isc1p and de novo synthesis catalyzed by Lag1p, and provide the first in vivo indication of its involvement in mitochondrial outer membrane permeabilization in yeast. Acetic Acid 84-95 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 206-211 15236759-6 2004 The two multicopy suppressors YBR183w (YPC1) and YPL087w (YDC1) encode ceramidases unrelated to Lag1p and Lac1p, which were previously found to support the reverse reaction of ceramide synthesis. Ceramides 176-184 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 96-101 20208441-6 2010 In this study, we investigated the effect of the genes involved in the synthesis of ceramide, lcb1, lcb2, tsc10, lac1, lag1, and sur2 on ceramide production levels. Ceramides 137-145 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 119-123 16987101-2 2007 The bulk of VLCFAs and DHS are used for ceramide synthesis by the Lag1p (longevity-assurance gene 1)/Lac1p (longevity-assurance gene cognate 1)/Lip1p (Lag1p/Lac1p interacting protein) ceramide synthase. Ceramides 40-48 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 66-71 16987101-2 2007 The bulk of VLCFAs and DHS are used for ceramide synthesis by the Lag1p (longevity-assurance gene 1)/Lac1p (longevity-assurance gene cognate 1)/Lip1p (Lag1p/Lac1p interacting protein) ceramide synthase. Ceramides 40-48 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 151-156 16303764-7 2006 Transfection with the yeast gene Lag1 Sc and the human homolog LASS1 increased ceramide levels and partially corrected growth and apoptosis in CLN9-deficient cells. Ceramides 79-87 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 33-37 16756512-10 2006 We also present evidence that histidine and aspartic acid residues in the Lag motif are essential for the function of Lag1p in vivo. Histidine 30-39 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 118-123 16756512-10 2006 We also present evidence that histidine and aspartic acid residues in the Lag motif are essential for the function of Lag1p in vivo. Aspartic Acid 44-57 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 118-123 12105227-0 2002 Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells. N-stearoylsphinganine 134-156 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 89-115 12200041-0 2002 LAG1 puts the focus on ceramide signaling. Ceramides 23-31 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 0-4 12200041-3 2002 The Lag 1 protein is located in the endoplasmic reticulum (ER), where it helps to accelerate the transport of glycosylphosphatidylinositol (GPI)-anchored proteins to the Golgi. Glycosylphosphatidylinositols 110-138 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 4-9 12200041-3 2002 The Lag 1 protein is located in the endoplasmic reticulum (ER), where it helps to accelerate the transport of glycosylphosphatidylinositol (GPI)-anchored proteins to the Golgi. Glycosylphosphatidylinositols 140-143 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 4-9 12200041-4 2002 This function of Lag 1 p results from its participation in ceramide synthesis. Ceramides 59-67 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 17-24 12200041-5 2002 Thus, Lag 1 p and its homologues are likely to play a role in ceramide signaling, which affects growth, proliferation, stress resistance, and apoptosis. Ceramides 62-70 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 6-13 12869556-0 2003 Human homologues of LAG1 reconstitute Acyl-CoA-dependent ceramide synthesis in yeast. Ceramides 57-65 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 20-24 12105227-0 2002 Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells. N-stearoylsphinganine 134-156 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 117-121 12105227-0 2002 Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells. N-stearoylsphinganine 158-179 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 89-115 12105227-0 2002 Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells. N-stearoylsphinganine 158-179 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 117-121 12105227-1 2002 The longevity assurance gene (LAG1) and its homolog (LAC1) are required for acyl-CoA-dependent synthesis of ceramides containing very long acyl chain (e.g. C26) fatty acids in yeast, and a homolog of LAG1, ASC1, confers resistance in plants to fumonisin B(1), an inhibitor of ceramide synthesis. Acyl Coenzyme A 76-84 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 200-204 12105227-1 2002 The longevity assurance gene (LAG1) and its homolog (LAC1) are required for acyl-CoA-dependent synthesis of ceramides containing very long acyl chain (e.g. C26) fatty acids in yeast, and a homolog of LAG1, ASC1, confers resistance in plants to fumonisin B(1), an inhibitor of ceramide synthesis. Ceramides 108-117 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 200-204 12105227-1 2002 The longevity assurance gene (LAG1) and its homolog (LAC1) are required for acyl-CoA-dependent synthesis of ceramides containing very long acyl chain (e.g. C26) fatty acids in yeast, and a homolog of LAG1, ASC1, confers resistance in plants to fumonisin B(1), an inhibitor of ceramide synthesis. Fatty Acids 161-172 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 200-204 12105227-1 2002 The longevity assurance gene (LAG1) and its homolog (LAC1) are required for acyl-CoA-dependent synthesis of ceramides containing very long acyl chain (e.g. C26) fatty acids in yeast, and a homolog of LAG1, ASC1, confers resistance in plants to fumonisin B(1), an inhibitor of ceramide synthesis. fumonisin b 244-255 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 200-204 12105227-1 2002 The longevity assurance gene (LAG1) and its homolog (LAC1) are required for acyl-CoA-dependent synthesis of ceramides containing very long acyl chain (e.g. C26) fatty acids in yeast, and a homolog of LAG1, ASC1, confers resistance in plants to fumonisin B(1), an inhibitor of ceramide synthesis. Ceramides 108-116 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 200-204 34492164-5 2022 In lip1-1 cells, Ypk1 was activated via both upstream kinases, TORC2 and yeast PDK1 homologues, Pkh1/2, thereby inducing hyperphosphorylation of Lag1, but not of another Ypk1-substrate, Orm1, which is a known negative regulator of the first step of sphingolipid metabolism, in the presence of Dox. Doxorubicin 293-296 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 145-149 11694577-4 2001 In this study, we show that lag1 Delta lac1 Delta cells have reduced sphingolipid levels due to a block of the fumonisin B1-sensitive and acyl-CoA-dependent ceramide synthase reaction. Sphingolipids 69-81 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 28-32 11694577-5 2001 The sphingolipid synthesis defect in lag1 Delta lac1 Delta cells can be partially corrected by overexpression of YPC1 or YDC1, encoding ceramidases that have been reported to have acyl-CoA-independent ceramide synthesis activity. Acyl Coenzyme A 180-188 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 37-41 11694577-5 2001 The sphingolipid synthesis defect in lag1 Delta lac1 Delta cells can be partially corrected by overexpression of YPC1 or YDC1, encoding ceramidases that have been reported to have acyl-CoA-independent ceramide synthesis activity. Ceramides 201-209 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 37-41 11694577-6 2001 Quadruple mutant cells (lag1 Delta lac1 Delta ypc1 Delta ydc1 Delta) do not make any sphingolipids, but are still viable probably because they produce novel lipids. Sphingolipids 85-98 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 24-28 11694577-7 2001 Moreover, lag1 Delta lac1 Delta cells are resistant to aureobasidin A, an inhibitor of the inositolphosphorylceramide synthase, suggesting that aureobasidin A may be toxic because it leads to increased ceramide levels. aureobasidin A 55-69 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 10-14 11694577-7 2001 Moreover, lag1 Delta lac1 Delta cells are resistant to aureobasidin A, an inhibitor of the inositolphosphorylceramide synthase, suggesting that aureobasidin A may be toxic because it leads to increased ceramide levels. aureobasidin A 144-158 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 10-14 11694577-7 2001 Moreover, lag1 Delta lac1 Delta cells are resistant to aureobasidin A, an inhibitor of the inositolphosphorylceramide synthase, suggesting that aureobasidin A may be toxic because it leads to increased ceramide levels. Ceramides 109-117 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 10-14 11387200-0 2001 C26-CoA-dependent ceramide synthesis of Saccharomyces cerevisiae is operated by Lag1p and Lac1p. Ceramides 18-26 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 80-85 11387200-3 2001 Here we show that microsomes or detergent extracts from lag1lac1 double mutants lack an activity transferring C26 fatty acids from C26-coenzyme A onto dihydrosphingosine or phytosphingosine. Fatty Acids 114-125 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 56-64 11387200-3 2001 Here we show that microsomes or detergent extracts from lag1lac1 double mutants lack an activity transferring C26 fatty acids from C26-coenzyme A onto dihydrosphingosine or phytosphingosine. safingol 151-169 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 56-64 11387200-3 2001 Here we show that microsomes or detergent extracts from lag1lac1 double mutants lack an activity transferring C26 fatty acids from C26-coenzyme A onto dihydrosphingosine or phytosphingosine. phytosphingosine 173-189 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 56-64 11387200-5 2001 lag1lac1 cells compensate for the lack of normal sphingolipids by making increased amounts of C26 fatty acids, which become incorporated into glycerophospholipids. Sphingolipids 49-62 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 0-8 11387200-5 2001 lag1lac1 cells compensate for the lack of normal sphingolipids by making increased amounts of C26 fatty acids, which become incorporated into glycerophospholipids. c26 fatty acids 94-109 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 0-8 11387200-5 2001 lag1lac1 cells compensate for the lack of normal sphingolipids by making increased amounts of C26 fatty acids, which become incorporated into glycerophospholipids. Glycerophospholipids 142-162 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 0-8 11387200-8 2001 The lipid remodelling of GPI-anchored proteins is severely compromised in lag1lac1 double mutants since only few and mostly abnormal ceramides are incorporated into the GPI anchors. Glycosylphosphatidylinositols 25-28 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 74-82 11387200-8 2001 The lipid remodelling of GPI-anchored proteins is severely compromised in lag1lac1 double mutants since only few and mostly abnormal ceramides are incorporated into the GPI anchors. Ceramides 133-142 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 74-82 11387200-8 2001 The lipid remodelling of GPI-anchored proteins is severely compromised in lag1lac1 double mutants since only few and mostly abnormal ceramides are incorporated into the GPI anchors. Glycosylphosphatidylinositols 169-172 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 74-82 11387200-9 2001 The participation of Lag1p and Lac1p in ceramide synthesis may explain their role in determining longevity. Ceramides 40-48 sphingosine N-acyltransferase LAG1 Saccharomyces cerevisiae S288C 21-26