PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 23691072-4 2013 In particular, potency and efficacy of DNA synthesis-targeting agents such as gemcitabine and etoposide could be profoundly underestimated by ATP and MTS-reduction assays. gemcitabine 78-89 MLRL Homo sapiens 150-153 23691072-4 2013 In particular, potency and efficacy of DNA synthesis-targeting agents such as gemcitabine and etoposide could be profoundly underestimated by ATP and MTS-reduction assays. Etoposide 94-103 MLRL Homo sapiens 150-153 8127183-4 1994 A cohort of MTS was treated with the chemopreventive agent retinoic acid (RA) to determine its effect on tumor cells. Tretinoin 59-72 MLRL Homo sapiens 12-15 22168771-3 2012 Here, we show the usefulness of duplex microcapsules with a Ca-alginate gel membrane as a platform for producing MTS with a highly homogeneous size distribution. ca-alginate 60-71 MLRL Homo sapiens 113-116 22168771-7 2012 Furthermore, the cells in MTS formed within microcapsules showed higher tolerance to mitomycin C (1-1000 nM) and gemcitabine (4.5-4500 nM) than 2-D cultured cells (P < 0.01). Mitomycin 85-96 MLRL Homo sapiens 26-29 22168771-7 2012 Furthermore, the cells in MTS formed within microcapsules showed higher tolerance to mitomycin C (1-1000 nM) and gemcitabine (4.5-4500 nM) than 2-D cultured cells (P < 0.01). gemcitabine 113-124 MLRL Homo sapiens 26-29 17487432-0 2007 Paradoxical proliferative potential of iron (II) sulphate on cancer cells after the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. ferrous sulfate 39-57 MLRL Homo sapiens 174-177 17487432-0 2007 Paradoxical proliferative potential of iron (II) sulphate on cancer cells after the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium 84-172 MLRL Homo sapiens 174-177 17487432-2 2007 [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) assay is one of the commonly used colourimetric absorption assays based on the ability of dehydrogenase from viable cells to produce the brown soluble formazan detectable at 490 nm. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium 1-89 MLRL Homo sapiens 92-95 17487432-2 2007 [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] (MTS) assay is one of the commonly used colourimetric absorption assays based on the ability of dehydrogenase from viable cells to produce the brown soluble formazan detectable at 490 nm. Formazans 248-256 MLRL Homo sapiens 92-95 17487432-3 2007 Here we have tested the possible growth influence of iron (II) sulphate on two human cancer cell lines, the K562 chronic myelogenous leukaemia and T47D breast carcinoma cells, based on the MTS assay. ferrous sulfate 53-71 MLRL Homo sapiens 189-192 17487432-8 2007 Interestingly, a consistent increment for the absorbance at 490 nm was further observed with increased iron (II) sulphate concentration either in the presence or absence of MTS even in a cell-free environment. ferrous sulfate 103-121 MLRL Homo sapiens 173-176 17487432-9 2007 Thus we conclude that iron (II) sulphate is actually growth inhibitory and even cytotoxic at high concentrations towards the K562 and T47D cancer cells and the paradoxical proliferative activity of iron (II) sulphate on these two cancer cell lines using the MTS assay was solely due to the oxidation of initial pale green iron (II) to brownish iron (III) during incubation in the aqueous condition. ferrous sulfate 22-40 MLRL Homo sapiens 258-261 17487432-9 2007 Thus we conclude that iron (II) sulphate is actually growth inhibitory and even cytotoxic at high concentrations towards the K562 and T47D cancer cells and the paradoxical proliferative activity of iron (II) sulphate on these two cancer cell lines using the MTS assay was solely due to the oxidation of initial pale green iron (II) to brownish iron (III) during incubation in the aqueous condition. Iron 22-28 MLRL Homo sapiens 258-261 17487432-9 2007 Thus we conclude that iron (II) sulphate is actually growth inhibitory and even cytotoxic at high concentrations towards the K562 and T47D cancer cells and the paradoxical proliferative activity of iron (II) sulphate on these two cancer cell lines using the MTS assay was solely due to the oxidation of initial pale green iron (II) to brownish iron (III) during incubation in the aqueous condition. Iron 22-26 MLRL Homo sapiens 258-261 14739608-0 2003 MTS-conjugated-antiactive caspase 3 antibodies inhibit actinomycin D-induced apoptosis. Dactinomycin 55-68 MLRL Homo sapiens 0-3 14739608-2 2003 We have conjugated a MTS-transport-peptide to monoclonal and polyclonal anti-caspase-3 antibodies to suppress Actinomycin D-induced apoptosis in human lymphoma T cells. Dactinomycin 110-123 MLRL Homo sapiens 21-24 14739608-4 2003 We could show that a MTS-transport-peptide modified monoclonal anti-caspase-3 antibody reduces Actinomycin D induced apoptosis, as shown by DNA ladder electrophoresis and cell death ELISA. Dactinomycin 95-108 MLRL Homo sapiens 21-24 12513744-2 2002 This automated colorimetric assay is based on the characteristic of viable and metabolically active leukemic cells to cleave MTS/pms into a water-soluble product whose optical density is determined at 492 nm by an automated microtiter-plate reader photometer. Water 140-145 MLRL Homo sapiens 125-128 12513744-5 2002 Compared with MTT and INT assays, the reduced product of MTS/pms is water-soluble. Water 68-73 MLRL Homo sapiens 57-60 9703889-1 1998 Human metallothioneins (hMTs), are low molecular weight cysteine-rich proteins that constitute the majority of intracellular protein thiols. Sulfhydryl Compounds 133-139 MLRL Homo sapiens 24-28 9703889-3 1998 Although their physiological function is not entirely understood, hMTs induction has been observed to be associated with protection from heavy metal toxicity and cellular resistance to cytotoxic anticancer drugs. Metals 143-148 MLRL Homo sapiens 66-70 8640448-0 1996 MTS interferon assay: a simplified cellular dehydrogenase assay for interferon activity using a water-soluble tetrazolium salt. Water 96-101 MLRL Homo sapiens 0-3 8640448-0 1996 MTS interferon assay: a simplified cellular dehydrogenase assay for interferon activity using a water-soluble tetrazolium salt. Tetrazolium Salts 110-126 MLRL Homo sapiens 0-3 8640448-1 1996 MTS, a tetrazolium dye, is reduced by hydrogenases in living cells to a water-soluble formazan. Tetrazolium Salts 7-18 MLRL Homo sapiens 0-3 8640448-1 1996 MTS, a tetrazolium dye, is reduced by hydrogenases in living cells to a water-soluble formazan. Water 72-77 MLRL Homo sapiens 0-3 8640448-1 1996 MTS, a tetrazolium dye, is reduced by hydrogenases in living cells to a water-soluble formazan. Formazans 86-94 MLRL Homo sapiens 0-3 8777059-0 1995 Aqueous soluble tetrazolium/formazan MTS as an indicator of NADH- and NADPH-dependent dehydrogenase activity. Formazans 28-36 MLRL Homo sapiens 37-40 8777059-0 1995 Aqueous soluble tetrazolium/formazan MTS as an indicator of NADH- and NADPH-dependent dehydrogenase activity. NAD 60-64 MLRL Homo sapiens 37-40 8777059-2 1995 This tetrazolium, commonly referred to as MTS [3-(4,5-dimethylthiazol-2-yl)- 5-(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], has the unusual property that it can be reduced to a water-soluble formazan. Tetrazolium Salts 5-16 MLRL Homo sapiens 42-45 8777059-2 1995 This tetrazolium, commonly referred to as MTS [3-(4,5-dimethylthiazol-2-yl)- 5-(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], has the unusual property that it can be reduced to a water-soluble formazan. 3-(4,5-dimethylthiazol-2-yl)- 5-(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2h-tetrazolium 47-136 MLRL Homo sapiens 42-45 8777059-2 1995 This tetrazolium, commonly referred to as MTS [3-(4,5-dimethylthiazol-2-yl)- 5-(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], has the unusual property that it can be reduced to a water-soluble formazan. Water 204-209 MLRL Homo sapiens 42-45 8777059-2 1995 This tetrazolium, commonly referred to as MTS [3-(4,5-dimethylthiazol-2-yl)- 5-(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt], has the unusual property that it can be reduced to a water-soluble formazan. Formazans 218-226 MLRL Homo sapiens 42-45 8777059-4 1995 In cell-free studies, MTS was reduce to the soluble formazan in the presence of NADH and NADPH, and reaction were compared to those with dithiothreitol (DTT) or 2-mercaptoethanol (2-ME). Formazans 52-60 MLRL Homo sapiens 22-25 8777059-4 1995 In cell-free studies, MTS was reduce to the soluble formazan in the presence of NADH and NADPH, and reaction were compared to those with dithiothreitol (DTT) or 2-mercaptoethanol (2-ME). NAD 80-84 MLRL Homo sapiens 22-25 8777059-4 1995 In cell-free studies, MTS was reduce to the soluble formazan in the presence of NADH and NADPH, and reaction were compared to those with dithiothreitol (DTT) or 2-mercaptoethanol (2-ME). NADP 89-94 MLRL Homo sapiens 22-25 8777059-4 1995 In cell-free studies, MTS was reduce to the soluble formazan in the presence of NADH and NADPH, and reaction were compared to those with dithiothreitol (DTT) or 2-mercaptoethanol (2-ME). Mercaptoethanol 180-184 MLRL Homo sapiens 22-25 8777059-7 1995 Generation of either NADH or NADPH in solution by malate dehydrogenase or isocitrate dehydrogenase, respectively, was monitored by the MTS reduction reaction. NAD 21-25 MLRL Homo sapiens 135-138 8777059-7 1995 Generation of either NADH or NADPH in solution by malate dehydrogenase or isocitrate dehydrogenase, respectively, was monitored by the MTS reduction reaction. NADP 29-34 MLRL Homo sapiens 135-138 8777059-9 1995 This system represents a useful tool for evaluating reaction kinetics in solutions of NAD- or NADP-dependent dehydrogenase enzymes, and these reactions can be performed in typical biological buffers containing reducing agents without significant interference to the MTS/formazan system. NAD 86-89 MLRL Homo sapiens 266-269 7868929-6 1995 Bioreduction of XTT and MTS usually requires addition of an intermediate electron acceptor, phenazine methosulphate (PMS). Methylphenazonium Methosulfate 92-115 MLRL Homo sapiens 24-27 7868929-6 1995 Bioreduction of XTT and MTS usually requires addition of an intermediate electron acceptor, phenazine methosulphate (PMS). Methylphenazonium Methosulfate 117-120 MLRL Homo sapiens 24-27 7868929-12 1995 We demonstrate that MTS/PMS provides microculture tetrazolium assays for hGH which are free from these serious artefacts and which are uniquely precise. Tetrazolium Salts 50-61 MLRL Homo sapiens 20-23 7868929-13 1995 In conclusion we therefore advocate the use of MTS in preference to XTT for the new generation of microculture tetrazolium assays. Tetrazolium Salts 111-122 MLRL Homo sapiens 47-50 9482120-0 1997 The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of 20 chemicals using human cell lines. Tetrazolium Salts 43-54 MLRL Homo sapiens 39-42 9066642-8 1997 Resistance to taxol and doxorubicin of the MDR cells grown as MTS was almost completely reversed by SDZ PSC 833. Paclitaxel 14-19 MLRL Homo sapiens 62-65 9066642-8 1997 Resistance to taxol and doxorubicin of the MDR cells grown as MTS was almost completely reversed by SDZ PSC 833. Doxorubicin 24-35 MLRL Homo sapiens 62-65 8127183-4 1994 A cohort of MTS was treated with the chemopreventive agent retinoic acid (RA) to determine its effect on tumor cells. Tretinoin 74-76 MLRL Homo sapiens 12-15 8127183-6 1994 Spectroscopic scans demonstrated consistently that RA-treated MTS exhibit a decrease in the peak associated with reduced nicotinamide-adenine dinucleotide (NADH) and an increase in the peaks associated with flavins, tryptophan, and cytokeratins when compared to controls. Tretinoin 51-53 MLRL Homo sapiens 62-65 8127183-6 1994 Spectroscopic scans demonstrated consistently that RA-treated MTS exhibit a decrease in the peak associated with reduced nicotinamide-adenine dinucleotide (NADH) and an increase in the peaks associated with flavins, tryptophan, and cytokeratins when compared to controls. NAD 121-154 MLRL Homo sapiens 62-65 8127183-6 1994 Spectroscopic scans demonstrated consistently that RA-treated MTS exhibit a decrease in the peak associated with reduced nicotinamide-adenine dinucleotide (NADH) and an increase in the peaks associated with flavins, tryptophan, and cytokeratins when compared to controls. NAD 156-160 MLRL Homo sapiens 62-65 8127183-6 1994 Spectroscopic scans demonstrated consistently that RA-treated MTS exhibit a decrease in the peak associated with reduced nicotinamide-adenine dinucleotide (NADH) and an increase in the peaks associated with flavins, tryptophan, and cytokeratins when compared to controls. Flavins 207-214 MLRL Homo sapiens 62-65 8127183-6 1994 Spectroscopic scans demonstrated consistently that RA-treated MTS exhibit a decrease in the peak associated with reduced nicotinamide-adenine dinucleotide (NADH) and an increase in the peaks associated with flavins, tryptophan, and cytokeratins when compared to controls. Tryptophan 216-226 MLRL Homo sapiens 62-65 34572561-6 2021 Bioinformatics analysis was performed using public databases, and the anti-proliferative ability of usenamine A in breast cancer cells was examined through MTS and colony formation assays. Usenamine A 100-111 MLRL Homo sapiens 156-159 33471566-4 2021 The cell viability of the cancer cells was assessed using the tetrazolium compound (MTS reagent), which was reduced to colored formazan, to indicate metabolically active cancer cells in a proliferating assay. Tetrazolium Salts 62-73 MLRL Homo sapiens 84-87 698922-1 1978 Multicellular tumor spheroids (MTS) have been exposed to chemotherapeutic agents in vitro (nitrogen mustard) or in vivo (cyclophosphamide) and analyzed in vitro in terms of altered growth patterns. Nitrogen 91-99 MLRL Homo sapiens 0-29 698922-1 1978 Multicellular tumor spheroids (MTS) have been exposed to chemotherapeutic agents in vitro (nitrogen mustard) or in vivo (cyclophosphamide) and analyzed in vitro in terms of altered growth patterns. Cyclophosphamide 121-137 MLRL Homo sapiens 0-29 698922-4 1978 injection cyclophosphamide results in patterns similar to the in vitro exposure system, except that a host anti-MTS reaction was detected. Cyclophosphamide 10-26 MLRL Homo sapiens 112-115 2715079-0 1989 Potentiation of radiation effects on multicellular tumor spheroids (MTS) of HeLa cells by lonidamine. lonidamine 90-100 MLRL Homo sapiens 68-71 2715079-4 1989 When the MTS were treated with lonidamine in combination with fractionated irradiation, remarkable enhancement of growth inhibition was observed at the drug concentration of 10 micrograms/ml. lonidamine 31-41 MLRL Homo sapiens 9-12 7438097-9 1980 Polyionic compounds known to affect cell surface charge were found to reduce the numbers of attached MTS. polyionic compounds 0-19 MLRL Homo sapiens 101-104 33471566-4 2021 The cell viability of the cancer cells was assessed using the tetrazolium compound (MTS reagent), which was reduced to colored formazan, to indicate metabolically active cancer cells in a proliferating assay. Formazans 127-135 MLRL Homo sapiens 84-87 29281029-10 2018 A colorimetric method using novel tetrazolium compound: MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] was used for cell activity and cytotoxicity assays. Tetrazolium Salts 34-45 MLRL Homo sapiens 56-59 29281029-10 2018 A colorimetric method using novel tetrazolium compound: MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt] was used for cell activity and cytotoxicity assays. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium 61-149 MLRL Homo sapiens 56-59 26837533-8 2016 MTS [(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl) 2-(4-sulfophenyl)-2H-tetrazolium] and a 5-ethynyl-2"-deoxyuridine (EdU) incorporation assay were used to detect AS fibroblasts proliferation after transfection. 5-ethynyl-2'-deoxyuridine 99-124 MLRL Homo sapiens 0-3 26837533-8 2016 MTS [(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl) 2-(4-sulfophenyl)-2H-tetrazolium] and a 5-ethynyl-2"-deoxyuridine (EdU) incorporation assay were used to detect AS fibroblasts proliferation after transfection. 5-ethynyl-2'-deoxyuridine 126-129 MLRL Homo sapiens 0-3 26574392-7 2015 We also calculated a potential of mean force (PMF) of a dihedral rotation for the MTS [(1-oxyl-2,2,5,5-tetramethyl-pyrroline-3-methyl)methanethiosulfonate] spin label via umbrella sampling with a set of regular MD trajectories, as well as a set of mass-repartitioned trajectories with a time step of 4 fs. (1-oxyl-2,2,5,5-tetramethylpyrroline-3-methyl)methanethiosulfonate 87-154 MLRL Homo sapiens 82-85 25473822-6 2015 We demonstrate the application of C&E analysis to the commonly used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell-viability assay. c& 34-39 MLRL Homo sapiens 162-165 25473822-6 2015 We demonstrate the application of C&E analysis to the commonly used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell-viability assay. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium 72-160 MLRL Homo sapiens 162-165