PMID-sentid	Pub_year	Sent_text	comp_official_name	comp_offset	protein_name	organism	prot_offset
31760159-0	2019	PBS3 and EPS1 Complete Salicylic Acid Biosynthesis from Isochorismate in Arabidopsis.	Salicylic Acid	23-37	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
31760159-6	2019	We found that PBS3, a GH3 acyl adenylase-family enzyme important for SA accumulation, catalyzes ATP- and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate, isochorismoyl-glutamate A.	Acyl Coenzyme A	26-30	Auxin-responsive GH3 family protein	Arabidopsis thaliana	14-18
31760159-6	2019	We found that PBS3, a GH3 acyl adenylase-family enzyme important for SA accumulation, catalyzes ATP- and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate, isochorismoyl-glutamate A.	Salicylic Acid	69-71	Auxin-responsive GH3 family protein	Arabidopsis thaliana	14-18
31760159-6	2019	We found that PBS3, a GH3 acyl adenylase-family enzyme important for SA accumulation, catalyzes ATP- and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate, isochorismoyl-glutamate A.	Adenosine Triphosphate	96-99	Auxin-responsive GH3 family protein	Arabidopsis thaliana	14-18
31760159-6	2019	We found that PBS3, a GH3 acyl adenylase-family enzyme important for SA accumulation, catalyzes ATP- and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate, isochorismoyl-glutamate A.	Magnesium	105-109	Auxin-responsive GH3 family protein	Arabidopsis thaliana	14-18
31760159-6	2019	We found that PBS3, a GH3 acyl adenylase-family enzyme important for SA accumulation, catalyzes ATP- and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate, isochorismoyl-glutamate A.	Glutamic Acid	135-146	Auxin-responsive GH3 family protein	Arabidopsis thaliana	14-18
31760159-6	2019	We found that PBS3, a GH3 acyl adenylase-family enzyme important for SA accumulation, catalyzes ATP- and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate, isochorismoyl-glutamate A.	carboxyl radical	164-174	Auxin-responsive GH3 family protein	Arabidopsis thaliana	14-18
31760159-6	2019	We found that PBS3, a GH3 acyl adenylase-family enzyme important for SA accumulation, catalyzes ATP- and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate, isochorismoyl-glutamate A.	Salicylic Acid	211-213	Auxin-responsive GH3 family protein	Arabidopsis thaliana	14-18
31760159-6	2019	We found that PBS3, a GH3 acyl adenylase-family enzyme important for SA accumulation, catalyzes ATP- and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate, isochorismoyl-glutamate A.	isochorismoyl-glutamate a	241-266	Auxin-responsive GH3 family protein	Arabidopsis thaliana	14-18
31760159-8	2019	Together, PBS3 and EPS1 form a two-step metabolic pathway to produce SA from isochorismate in Arabidopsis, which is distinct from how SA is biosynthesized in bacteria.	Salicylic Acid	69-71	Auxin-responsive GH3 family protein	Arabidopsis thaliana	10-14
31760159-8	2019	Together, PBS3 and EPS1 form a two-step metabolic pathway to produce SA from isochorismate in Arabidopsis, which is distinct from how SA is biosynthesized in bacteria.	Salicylic Acid	134-136	Auxin-responsive GH3 family protein	Arabidopsis thaliana	10-14
31575658-3	2019	One Brassicaceae-specific clade of GH3 proteins was predicted to use benzoate as a substrate and includes AtGH3.7 and AtGH3.12/PBS3.	Benzoates	69-77	Auxin-responsive GH3 family protein	Arabidopsis thaliana	127-131
31575658-4	2019	Previously identified as a 4-hydroxybenzoic acid-glutamate synthetase, AtGH3.12/PBS3 influences pathogen defense responses through salicylic acid.	4-hydroxybenzoic acid	27-48	Auxin-responsive GH3 family protein	Arabidopsis thaliana	80-84
31575658-4	2019	Previously identified as a 4-hydroxybenzoic acid-glutamate synthetase, AtGH3.12/PBS3 influences pathogen defense responses through salicylic acid.	Glutamic Acid	49-58	Auxin-responsive GH3 family protein	Arabidopsis thaliana	80-84
31575658-4	2019	Previously identified as a 4-hydroxybenzoic acid-glutamate synthetase, AtGH3.12/PBS3 influences pathogen defense responses through salicylic acid.	Salicylic Acid	131-145	Auxin-responsive GH3 family protein	Arabidopsis thaliana	80-84
31575658-5	2019	Recent work has shown that AtGH3.12/PBS3 uses isochorismate as a substrate, forming an isochorismate-glutamate conjugate that converts into salicylic acid.	Glutamic Acid	101-110	Auxin-responsive GH3 family protein	Arabidopsis thaliana	36-40
31575658-5	2019	Recent work has shown that AtGH3.12/PBS3 uses isochorismate as a substrate, forming an isochorismate-glutamate conjugate that converts into salicylic acid.	Salicylic Acid	140-154	Auxin-responsive GH3 family protein	Arabidopsis thaliana	36-40
31575658-6	2019	Here, we show that AtGH3.7 and AtGH3.12/PBS3 can also conjugate chorismate, a precursor of aromatic amino acids and salicylic acid, to cysteine and glutamate, respectively.	Aromatic Amino Acid Decarboxylase Inhibitors	91-111	Auxin-responsive GH3 family protein	Arabidopsis thaliana	40-44
31575658-6	2019	Here, we show that AtGH3.7 and AtGH3.12/PBS3 can also conjugate chorismate, a precursor of aromatic amino acids and salicylic acid, to cysteine and glutamate, respectively.	Salicylic Acid	116-130	Auxin-responsive GH3 family protein	Arabidopsis thaliana	40-44
31575658-6	2019	Here, we show that AtGH3.7 and AtGH3.12/PBS3 can also conjugate chorismate, a precursor of aromatic amino acids and salicylic acid, to cysteine and glutamate, respectively.	Cysteine	135-143	Auxin-responsive GH3 family protein	Arabidopsis thaliana	40-44
31575658-6	2019	Here, we show that AtGH3.7 and AtGH3.12/PBS3 can also conjugate chorismate, a precursor of aromatic amino acids and salicylic acid, to cysteine and glutamate, respectively.	Glutamic Acid	148-157	Auxin-responsive GH3 family protein	Arabidopsis thaliana	40-44
31575658-7	2019	The X-ray crystal structure of AtGH3.12/PBS3 in complex with AMP and chorismate at 1.94 A resolution, along with site-directed mutagenesis, revealed how the active site potentially accommodates this substrate.	Adenosine Monophosphate	61-64	Auxin-responsive GH3 family protein	Arabidopsis thaliana	40-44
31371615-5	2019	We show that in Arabidopsis, the pathway downstream of isochorismate requires only two additional proteins: ENHANCED DISEASE SUSCEPTIBILITY5, which exports isochorismate from the plastid to the cytosol, and the cytosolic amidotransferase avrPphB SUSCEPTIBLE3 (PBS3).	isochorismic acid	55-68	Auxin-responsive GH3 family protein	Arabidopsis thaliana	260-264
31371615-5	2019	We show that in Arabidopsis, the pathway downstream of isochorismate requires only two additional proteins: ENHANCED DISEASE SUSCEPTIBILITY5, which exports isochorismate from the plastid to the cytosol, and the cytosolic amidotransferase avrPphB SUSCEPTIBLE3 (PBS3).	isochorismic acid	156-169	Auxin-responsive GH3 family protein	Arabidopsis thaliana	260-264
31371615-6	2019	PBS3 catalyzes the conjugation of glutamate to isochorismate to produce isochorismate-9-glutamate, which spontaneously decomposes into SA and 2-hydroxy-acryloyl-N-glutamate.	Glutamic Acid	34-43	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
31371615-6	2019	PBS3 catalyzes the conjugation of glutamate to isochorismate to produce isochorismate-9-glutamate, which spontaneously decomposes into SA and 2-hydroxy-acryloyl-N-glutamate.	isochorismic acid	47-60	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
31371615-6	2019	PBS3 catalyzes the conjugation of glutamate to isochorismate to produce isochorismate-9-glutamate, which spontaneously decomposes into SA and 2-hydroxy-acryloyl-N-glutamate.	isochorismate-9-glutamate	72-97	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
31371615-6	2019	PBS3 catalyzes the conjugation of glutamate to isochorismate to produce isochorismate-9-glutamate, which spontaneously decomposes into SA and 2-hydroxy-acryloyl-N-glutamate.	Salicylic Acid	135-137	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
31371615-6	2019	PBS3 catalyzes the conjugation of glutamate to isochorismate to produce isochorismate-9-glutamate, which spontaneously decomposes into SA and 2-hydroxy-acryloyl-N-glutamate.	2-hydroxy-acryloyl-n-glutamate	142-172	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
30674663-3	2019	Abiotic stresses, such as high salinity and drought, blunted immune responses in older rosette leaves through the phytohormone abscisic acid signaling, whereas this antagonistic effect was blocked in younger rosette leaves by PBS3, a signaling component of the defense phytohormone salicylic acid.	Salicylic Acid	282-296	Auxin-responsive GH3 family protein	Arabidopsis thaliana	226-230
30674663-5	2019	Together with this role, PBS3 is also indispensable for the establishment of salt stress- and leaf age-dependent phyllosphere bacterial communities.	Salts	77-81	Auxin-responsive GH3 family protein	Arabidopsis thaliana	25-29
21595875-4	2011	Also AVRPPHB SUSCEPTIBLE 3 (PBS3) plays an important role in SA metabolism, as pbs3 mutants accumulate drastically reduced levels of SA-glucoside, a putative storage form of SA.	Salicylic Acid	61-63	Auxin-responsive GH3 family protein	Arabidopsis thaliana	28-32
28743075-4	2017	For example, we found GH3.5 activity on substituted benzoates is not defined by the substitution position as it is for GH3.12/PBS3.	3'-dGTP	22-25	Auxin-responsive GH3 family protein	Arabidopsis thaliana	126-130
21594748-5	2011	Combining analysis of synteny with phylogenetics, gene expression and functional data redefines the Group III GH3 genes, of which AtGH3.12/PBS3, a regulator of stress-induced salicylic acid metabolism and plant defense, is a member.	Salicylic Acid	175-189	Auxin-responsive GH3 family protein	Arabidopsis thaliana	139-143
21543726-1	2011	The salicylic acid (SA) regulatory gene HOPW1-1-INTERACTING3 (WIN3) was previously shown to confer resistance to the biotrophic pathogen Pseudomonas syringae.	Salicylic Acid	4-18	Auxin-responsive GH3 family protein	Arabidopsis thaliana	62-66
21543726-1	2011	The salicylic acid (SA) regulatory gene HOPW1-1-INTERACTING3 (WIN3) was previously shown to confer resistance to the biotrophic pathogen Pseudomonas syringae.	Salicylic Acid	20-22	Auxin-responsive GH3 family protein	Arabidopsis thaliana	62-66
21543726-3	2011	Genetic analysis indicates that WIN3 acts additively with several known SA regulators, including PHYTOALEXIN DEFICIENT4, NONEXPRESSOR OF PR GENES1 (NPR1), and SA INDUCTION-DEFICIENT2, in regulating SA accumulation, cell death, and/or disease resistance in the Arabidopsis (Arabidopsis thaliana) mutant acd6-1.	Salicylic Acid	72-74	Auxin-responsive GH3 family protein	Arabidopsis thaliana	32-36
24100325-6	2013	Here, the X-ray crystal structure of GH3.12 is presented in the closed conformation in complex with the nonhydrolysable ATP analogue AMPCPP and the substrate salicylate.	Adenosine Triphosphate	120-123	Auxin-responsive GH3 family protein	Arabidopsis thaliana	37-43
24100325-6	2013	Here, the X-ray crystal structure of GH3.12 is presented in the closed conformation in complex with the nonhydrolysable ATP analogue AMPCPP and the substrate salicylate.	alpha,beta-methyleneadenosine 5'-triphosphate	133-139	Auxin-responsive GH3 family protein	Arabidopsis thaliana	37-43
24100325-6	2013	Here, the X-ray crystal structure of GH3.12 is presented in the closed conformation in complex with the nonhydrolysable ATP analogue AMPCPP and the substrate salicylate.	Salicylates	158-168	Auxin-responsive GH3 family protein	Arabidopsis thaliana	37-43
22628555-2	2012	Here, we report the crystal structures of benzoate-specific Arabidopsis thaliana AtGH3.12/PBS3 and jasmonic acid-specific AtGH3.11/JAR1.	Benzoates	42-50	Auxin-responsive GH3 family protein	Arabidopsis thaliana	90-94
21595875-4	2011	Also AVRPPHB SUSCEPTIBLE 3 (PBS3) plays an important role in SA metabolism, as pbs3 mutants accumulate drastically reduced levels of SA-glucoside, a putative storage form of SA.	Salicylic Acid	61-63	Auxin-responsive GH3 family protein	Arabidopsis thaliana	79-83
21595875-4	2011	Also AVRPPHB SUSCEPTIBLE 3 (PBS3) plays an important role in SA metabolism, as pbs3 mutants accumulate drastically reduced levels of SA-glucoside, a putative storage form of SA.	salicylic acid glucoside	133-145	Auxin-responsive GH3 family protein	Arabidopsis thaliana	28-32
21595875-4	2011	Also AVRPPHB SUSCEPTIBLE 3 (PBS3) plays an important role in SA metabolism, as pbs3 mutants accumulate drastically reduced levels of SA-glucoside, a putative storage form of SA.	salicylic acid glucoside	133-145	Auxin-responsive GH3 family protein	Arabidopsis thaliana	79-83
21595875-4	2011	Also AVRPPHB SUSCEPTIBLE 3 (PBS3) plays an important role in SA metabolism, as pbs3 mutants accumulate drastically reduced levels of SA-glucoside, a putative storage form of SA.	Salicylic Acid	133-135	Auxin-responsive GH3 family protein	Arabidopsis thaliana	28-32
21595875-4	2011	Also AVRPPHB SUSCEPTIBLE 3 (PBS3) plays an important role in SA metabolism, as pbs3 mutants accumulate drastically reduced levels of SA-glucoside, a putative storage form of SA.	Salicylic Acid	133-135	Auxin-responsive GH3 family protein	Arabidopsis thaliana	79-83
19816125-9	2009	Two recently identified Arabidopsis genes, PBS3 and EPS1, are important for pathogen-induced SA accumulation.	Salicylic Acid	93-95	Auxin-responsive GH3 family protein	Arabidopsis thaliana	43-47
19816125-11	2009	PBS3 and EPS1 may be directly involved in the synthesis of an important precursor or regulatory molecule for SA biosynthesis.	Salicylic Acid	109-111	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
17468220-0	2007	The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis.	Salicylic Acid	52-66	Auxin-responsive GH3 family protein	Arabidopsis thaliana	37-41
19189963-0	2009	Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate.	4-substituted benzoates	52-75	Auxin-responsive GH3 family protein	Arabidopsis thaliana	12-18
19189963-0	2009	Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate.	4-substituted benzoates	52-75	Auxin-responsive GH3 family protein	Arabidopsis thaliana	20-24
19189963-0	2009	Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate.	Salicylates	96-106	Auxin-responsive GH3 family protein	Arabidopsis thaliana	12-18
19189963-0	2009	Arabidopsis GH3.12 (PBS3) conjugates amino acids to 4-substituted benzoates and is inhibited by salicylate.	Salicylates	96-106	Auxin-responsive GH3 family protein	Arabidopsis thaliana	20-24
19189963-3	2009	In gh3.12/pbs3 mutants of Arabidopsis thaliana, induced total SA accumulation is significantly compromised as is SA-dependent gene expression and plant defense.	Salicylates	62-64	Auxin-responsive GH3 family protein	Arabidopsis thaliana	3-9
19189963-3	2009	In gh3.12/pbs3 mutants of Arabidopsis thaliana, induced total SA accumulation is significantly compromised as is SA-dependent gene expression and plant defense.	Salicylates	62-64	Auxin-responsive GH3 family protein	Arabidopsis thaliana	10-14
19189963-3	2009	In gh3.12/pbs3 mutants of Arabidopsis thaliana, induced total SA accumulation is significantly compromised as is SA-dependent gene expression and plant defense.	Salicylates	113-115	Auxin-responsive GH3 family protein	Arabidopsis thaliana	3-9
19189963-3	2009	In gh3.12/pbs3 mutants of Arabidopsis thaliana, induced total SA accumulation is significantly compromised as is SA-dependent gene expression and plant defense.	Salicylates	113-115	Auxin-responsive GH3 family protein	Arabidopsis thaliana	10-14
19189963-7	2009	We found PBS3 favors 4-substituted benzoates such as 4-aminobenzoate and 4-hydroxybenzoate, with moderate activity on benzoate and no observed activity with 2-substituted benzoates.	4-substituted benzoates	21-44	Auxin-responsive GH3 family protein	Arabidopsis thaliana	9-13
19189963-7	2009	We found PBS3 favors 4-substituted benzoates such as 4-aminobenzoate and 4-hydroxybenzoate, with moderate activity on benzoate and no observed activity with 2-substituted benzoates.	para-Aminobenzoates	53-68	Auxin-responsive GH3 family protein	Arabidopsis thaliana	9-13
19189963-7	2009	We found PBS3 favors 4-substituted benzoates such as 4-aminobenzoate and 4-hydroxybenzoate, with moderate activity on benzoate and no observed activity with 2-substituted benzoates.	4-hydroxybenzoic acid	73-90	Auxin-responsive GH3 family protein	Arabidopsis thaliana	9-13
19189963-7	2009	We found PBS3 favors 4-substituted benzoates such as 4-aminobenzoate and 4-hydroxybenzoate, with moderate activity on benzoate and no observed activity with 2-substituted benzoates.	Benzoates	35-43	Auxin-responsive GH3 family protein	Arabidopsis thaliana	9-13
19189963-8	2009	Similar to known GH3 enzymes, PBS3 catalyzes the conjugation of specific amino acids (e.g. Glu) to its preferred acyl substrates.	3'-dGTP	17-20	Auxin-responsive GH3 family protein	Arabidopsis thaliana	30-34
19189963-8	2009	Similar to known GH3 enzymes, PBS3 catalyzes the conjugation of specific amino acids (e.g. Glu) to its preferred acyl substrates.	Glutamic Acid	91-94	Auxin-responsive GH3 family protein	Arabidopsis thaliana	30-34
19189963-10	2009	Notably, SA specifically and reversibly inhibits PBS3 activity with an IC(50) of 15 microm.	Salicylates	9-11	Auxin-responsive GH3 family protein	Arabidopsis thaliana	49-53
19189963-12	2009	For PBS3, this may allow for coordination of flux through diverse chorismate-derived pathways.	Chorismic Acid	66-76	Auxin-responsive GH3 family protein	Arabidopsis thaliana	4-8
17918621-8	2007	WIN3 expression in tissue distal to an infection site requires multiple salicylic acid regulatory genes.	Salicylic Acid	72-86	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
17468220-3	2007	Surprisingly, whereas accumulation of the SA glucoside and expression of PR1 were dramatically reduced in the pbs3-1 mutant in response to P. syringae (avrRpt2) infection, free SA was elevated.	salicylic acid glucoside	42-54	Auxin-responsive GH3 family protein	Arabidopsis thaliana	110-114
17468220-3	2007	Surprisingly, whereas accumulation of the SA glucoside and expression of PR1 were dramatically reduced in the pbs3-1 mutant in response to P. syringae (avrRpt2) infection, free SA was elevated.	Salicylic Acid	42-44	Auxin-responsive GH3 family protein	Arabidopsis thaliana	110-114
17468220-4	2007	However, in response to exogenous SA, the conversion of free SA to SA glucoside and the induced expression of PR1 were similar in pbs3-1 and wild-type plants.	Salicylic Acid	34-36	Auxin-responsive GH3 family protein	Arabidopsis thaliana	130-134
17468220-7	2007	PBS3 (also referred to as GH3.12) is a member of the GH3 family of acyl-adenylate/thioester-forming enzymes.	3'-dGTP	26-29	Auxin-responsive GH3 family protein	Arabidopsis thaliana	0-4
17468220-9	2007	Thus, our results suggest that amino acid conjugation plays a critical role in SA metabolism and induced defense responses, with PBS3 acting upstream of SA, directly on SA, or on a competitive inhibitor of SA.	Salicylic Acid	153-155	Auxin-responsive GH3 family protein	Arabidopsis thaliana	129-133
17468220-9	2007	Thus, our results suggest that amino acid conjugation plays a critical role in SA metabolism and induced defense responses, with PBS3 acting upstream of SA, directly on SA, or on a competitive inhibitor of SA.	Salicylic Acid	153-155	Auxin-responsive GH3 family protein	Arabidopsis thaliana	129-133
17468220-9	2007	Thus, our results suggest that amino acid conjugation plays a critical role in SA metabolism and induced defense responses, with PBS3 acting upstream of SA, directly on SA, or on a competitive inhibitor of SA.	Salicylic Acid	153-155	Auxin-responsive GH3 family protein	Arabidopsis thaliana	129-133
10224270-9	1999	The pbs mutations are recessive and have been mapped to chromosomes I (pbs2) and V (pbs1 and pbs3).	Lead	4-7	Auxin-responsive GH3 family protein	Arabidopsis thaliana	93-97