PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 3023378-14 1986 Since this antiestrogen is a complete antagonist in the chick oviduct and prevents estradiol-induced stimulation of ovalbumin gene transcription, it is speculated that Rx to Ry conversion is crucial for ovalbumin gene activation and that Rx may act as a transcriptional repressor. Estradiol 83-92 ovalbumin (SERPINB14) Gallus gallus 116-125 2780293-1 1989 To understand the molecular basis of the steroid hormone regulated expression of the ovalbumin gene, we sought to identify and isolate nuclear factors from chicken oviduct which interact specifically with the ovalbumin promoter. Steroids 41-48 ovalbumin (SERPINB14) Gallus gallus 85-94 2780293-1 1989 To understand the molecular basis of the steroid hormone regulated expression of the ovalbumin gene, we sought to identify and isolate nuclear factors from chicken oviduct which interact specifically with the ovalbumin promoter. Steroids 41-48 ovalbumin (SERPINB14) Gallus gallus 209-218 3410855-1 1988 Substitution of glutamine for asparagine 293 in chicken ovalbumin does not allow glycosylation of asparagine 312. Asparagine 30-40 ovalbumin (SERPINB14) Gallus gallus 56-65 3410855-2 1988 It has been shown previously that chicken ovalbumin synthesized and secreted in a heterologous cell system is glycosylated at the correct site and that the oligosaccharides at that site, similar to the protein made in hen oviduct, are predominantly of the hybrid type (Sheares, B. T., and Robbins, P. W. (1986) Proc. Oligosaccharides 156-172 ovalbumin (SERPINB14) Gallus gallus 42-51 3410855-9 1988 Using a 20-base oligodeoxynucleotide primer containing a 2-base mismatch, the codon for Asn293 in the chicken ovalbumin gene (AAC) was changed to that for Gln (CAA), thereby preventing glycosylation at amino acid 293. Glutamine 155-158 ovalbumin (SERPINB14) Gallus gallus 110-119 3410855-11 1988 Ovalbumin secreted by these cells was recovered by immunoaffinity chromatography and analyzed for the presence of an oligosaccharide attached at Asn312. Oligosaccharides 117-132 ovalbumin (SERPINB14) Gallus gallus 0-9 3386256-1 1988 The relative rate of ovalbumin transcription was significantly increased (P less than 0.001) when purified chick liver glucocorticosteroid receptor (GR) was incubated with purified nuclei prepared from the oviducts of diethylstilboestrol (DES)-primed chickens 24 h after oestrogen withdrawal. diethylstilboestrol 218-237 ovalbumin (SERPINB14) Gallus gallus 21-30 3386256-1 1988 The relative rate of ovalbumin transcription was significantly increased (P less than 0.001) when purified chick liver glucocorticosteroid receptor (GR) was incubated with purified nuclei prepared from the oviducts of diethylstilboestrol (DES)-primed chickens 24 h after oestrogen withdrawal. desacetyluvaricin 239-242 ovalbumin (SERPINB14) Gallus gallus 21-30 3047126-7 1988 The purine contacts in the two promoters are limited to one face of the DNA helix; however, studies on phosphate contacts suggest that the COUP transcription factor wraps around the ovalbumin promoter, while it binds to only one face of the DNA helix in the insulin promoter. purine 4-10 ovalbumin (SERPINB14) Gallus gallus 182-191 3047126-7 1988 The purine contacts in the two promoters are limited to one face of the DNA helix; however, studies on phosphate contacts suggest that the COUP transcription factor wraps around the ovalbumin promoter, while it binds to only one face of the DNA helix in the insulin promoter. Phosphates 103-112 ovalbumin (SERPINB14) Gallus gallus 182-191 3064812-0 1988 Positive and negative regulatory elements control the steroid-responsive ovalbumin promoter. Steroids 54-61 ovalbumin (SERPINB14) Gallus gallus 73-82 3064812-1 1988 Steroid hormones regulate the transcriptional activity of the chicken ovalbumin gene both in vivo and in cell culture. Steroids 0-16 ovalbumin (SERPINB14) Gallus gallus 70-79 3064812-3 1988 Induction of the OvCAT genes by estrogen, progesterone, or corticosterone mimics that of the endogenous ovalbumin gene, indicating that the transfected DNA is accurately regulated. Progesterone 42-54 ovalbumin (SERPINB14) Gallus gallus 104-113 3064812-3 1988 Induction of the OvCAT genes by estrogen, progesterone, or corticosterone mimics that of the endogenous ovalbumin gene, indicating that the transfected DNA is accurately regulated. Corticosterone 59-73 ovalbumin (SERPINB14) Gallus gallus 104-113 3064812-5 1988 Thus, an NRE represses expression of the ovalbumin gene unless steroid hormones relieve this negative control through interactions involving a more distal SRE. Steroids 63-79 ovalbumin (SERPINB14) Gallus gallus 41-50 3396330-2 1988 Ovalbumin was fractionated to six fractions according to their phosphate content by high performance anion exchange chromatography. Phosphates 63-72 ovalbumin (SERPINB14) Gallus gallus 0-9 3396330-4 1988 This method was applied to analyze the phosphate content of ovalbumin subfractions having different carbohydrate chain from each other which were prepared by concanavalin A/Sepharose chromatography from oviduct slices incubated with [2-3H]mannose. Phosphates 39-48 ovalbumin (SERPINB14) Gallus gallus 60-69 3396330-4 1988 This method was applied to analyze the phosphate content of ovalbumin subfractions having different carbohydrate chain from each other which were prepared by concanavalin A/Sepharose chromatography from oviduct slices incubated with [2-3H]mannose. Carbohydrates 100-112 ovalbumin (SERPINB14) Gallus gallus 60-69 3396330-4 1988 This method was applied to analyze the phosphate content of ovalbumin subfractions having different carbohydrate chain from each other which were prepared by concanavalin A/Sepharose chromatography from oviduct slices incubated with [2-3H]mannose. [2-3h]mannose 233-246 ovalbumin (SERPINB14) Gallus gallus 60-69 3311742-8 1987 Tryptic peptides produced from the ovalbumin and serum albumin derivatives were fractionated by HPLC and subsequently analysed by amino acid analysis, FAB-MS and automated stepwise protein sequence analysis. Peptides 8-16 ovalbumin (SERPINB14) Gallus gallus 35-44 3665877-0 1987 The chicken ovalbumin promoter is under negative control which is relieved by steroid hormones. Steroids 78-94 ovalbumin (SERPINB14) Gallus gallus 12-21 3665877-1 1987 Steroid hormone regulation of activity of the chicken ovalbumin promoter was studied by microinjection of chimeric genes into the nuclei of primary cultured oviduct tubular gland cells. Steroids 0-15 ovalbumin (SERPINB14) Gallus gallus 54-63 3665877-4 1987 The activity of the ovalbumin promoter is cell-specifically repressed in these oviduct cells and the repression is relieved upon addition of steroid hormones. Steroids 141-157 ovalbumin (SERPINB14) Gallus gallus 20-29 3665877-5 1987 The -132 to -425 region of the ovalbumin promoter which is responsible for this negative regulation behaves as an independent functional unit containing the regulatory elements necessary for both repression (in the presence of steroid hormone antagonists) and induced derepression (in the presence of steroid hormones) of linked heterologous promoters. Steroids 227-242 ovalbumin (SERPINB14) Gallus gallus 31-40 3665877-5 1987 The -132 to -425 region of the ovalbumin promoter which is responsible for this negative regulation behaves as an independent functional unit containing the regulatory elements necessary for both repression (in the presence of steroid hormone antagonists) and induced derepression (in the presence of steroid hormones) of linked heterologous promoters. Steroids 301-317 ovalbumin (SERPINB14) Gallus gallus 31-40 3023378-14 1986 Since this antiestrogen is a complete antagonist in the chick oviduct and prevents estradiol-induced stimulation of ovalbumin gene transcription, it is speculated that Rx to Ry conversion is crucial for ovalbumin gene activation and that Rx may act as a transcriptional repressor. Estradiol 83-92 ovalbumin (SERPINB14) Gallus gallus 203-212 3021132-1 1986 By means of the DNA-cellulose competitive binding assay, the interaction of estrogen receptor complexed to 17 beta-estradiol with fragments of a cloned DNA region of the estrogen responsive chicken ovalbumin gene spanning from 1343 bps upstream to 373 bps within the transcribed region of the gene (p0V 1.7) was investigated. Cellulose 20-29 ovalbumin (SERPINB14) Gallus gallus 198-207 3801455-1 1986 The dependence of chromatin conformation upon salt concentration has been studied for chicken ovalbumin and beta-globin genes isolated from oviduct and adult erythrocytes. Salts 46-50 ovalbumin (SERPINB14) Gallus gallus 94-103 3021132-1 1986 By means of the DNA-cellulose competitive binding assay, the interaction of estrogen receptor complexed to 17 beta-estradiol with fragments of a cloned DNA region of the estrogen responsive chicken ovalbumin gene spanning from 1343 bps upstream to 373 bps within the transcribed region of the gene (p0V 1.7) was investigated. Estradiol 110-124 ovalbumin (SERPINB14) Gallus gallus 198-207 3487084-2 1986 We used the chicken ovalbumin peptide ovalbumin-(323-339)-Tyr, which is immunogenic in the BALB/c mouse and restricted to I-Ad. Tyrosine 58-61 ovalbumin (SERPINB14) Gallus gallus 20-29 3487084-2 1986 We used the chicken ovalbumin peptide ovalbumin-(323-339)-Tyr, which is immunogenic in the BALB/c mouse and restricted to I-Ad. Tyrosine 58-61 ovalbumin (SERPINB14) Gallus gallus 38-47 3487084-4 1986 This binding was inhibited by unlabeled ovalbumin-(323-339) but not by ovalbumin-(329-339), which is the longest N-terminally truncated peptide that fails to stimulate any of the I-Ad-restricted hybridomas that have been raised to ovalbumin-(323-339)-Tyr. Tyrosine 251-254 ovalbumin (SERPINB14) Gallus gallus 40-49 3707933-8 1986 The lower affinity receptor sediments at 3.5 S on sucrose density gradients compared to 4.2 S for the high-affinity form; it has high specificity for estrogen and is tissue specific, and its nuclear occupancy is associated with increases in ovalbumin gene transcription; thus, this macromolecule meets the criteria of an estrogen receptor. Sucrose 50-57 ovalbumin (SERPINB14) Gallus gallus 241-250 3457370-4 1986 As in the case of chicken ovalbumin, the protein synthesized in L cells contains large amounts of hybrid oligosaccharides. Oligosaccharides 105-121 ovalbumin (SERPINB14) Gallus gallus 26-35 3457370-6 1986 These results suggest that it is the polypeptide chain of ovalbumin that is responsible for proper glycosylation and subsequent processing of a substantial fraction of the oligosaccharide chains to hybrid structures. Oligosaccharides 172-187 ovalbumin (SERPINB14) Gallus gallus 58-67 3457370-5 1986 Approximately 50% of the [3H]mannose incorporated into ovalbumin secreted by L cells is found in such hybrid structures. Tritium 26-28 ovalbumin (SERPINB14) Gallus gallus 55-64 3457370-8 1986 The hybrid oligosaccharides of ovalbumin secreted by L cells are completely sialylated and do not contain a bisecting GlcNAc residue, distinguishing them from hybrid chains in chicken ovalbumin. Oligosaccharides 11-27 ovalbumin (SERPINB14) Gallus gallus 31-40 3457370-9 1986 In addition to high-mannose and hybrid oligosaccharide chains, ovalbumin synthesized in L cells contains oligosaccharides of the complex type. Oligosaccharides 105-121 ovalbumin (SERPINB14) Gallus gallus 63-72 3457370-5 1986 Approximately 50% of the [3H]mannose incorporated into ovalbumin secreted by L cells is found in such hybrid structures. Mannose 29-36 ovalbumin (SERPINB14) Gallus gallus 55-64 3457370-11 1986 These differences in fine structure, between the oligosaccharides derived from ovalbumin secreted by L cells and those known to be present in the chicken egg glycoprotein, suggest that the cell type also plays a role in oligosaccharide processing. Oligosaccharides 49-65 ovalbumin (SERPINB14) Gallus gallus 79-88 2858488-2 1985 At least 50% of the poly A+ mRNA molecules (9S rabbit globin mRNA, chicken ovalbumin, and lysozyme) were stable in oocytes over a 48-h period, irrespective of the amount injected. Poly A 20-26 ovalbumin (SERPINB14) Gallus gallus 75-84 3457370-11 1986 These differences in fine structure, between the oligosaccharides derived from ovalbumin secreted by L cells and those known to be present in the chicken egg glycoprotein, suggest that the cell type also plays a role in oligosaccharide processing. Oligosaccharides 49-64 ovalbumin (SERPINB14) Gallus gallus 79-88 3702411-10 1986 In the presence of estradiol (50 and 100 nM), progesterone (50 nM), and both estradiol and progesterone together (50 nM of each), ovalbumin and conalbumin synthesis was increased, when compared to control cultures without hormones, or to oviduct fibroblasts. Estradiol 19-28 ovalbumin (SERPINB14) Gallus gallus 130-139 3702411-10 1986 In the presence of estradiol (50 and 100 nM), progesterone (50 nM), and both estradiol and progesterone together (50 nM of each), ovalbumin and conalbumin synthesis was increased, when compared to control cultures without hormones, or to oviduct fibroblasts. Progesterone 46-58 ovalbumin (SERPINB14) Gallus gallus 130-139 3702411-10 1986 In the presence of estradiol (50 and 100 nM), progesterone (50 nM), and both estradiol and progesterone together (50 nM of each), ovalbumin and conalbumin synthesis was increased, when compared to control cultures without hormones, or to oviduct fibroblasts. Estradiol 77-86 ovalbumin (SERPINB14) Gallus gallus 130-139 3702411-10 1986 In the presence of estradiol (50 and 100 nM), progesterone (50 nM), and both estradiol and progesterone together (50 nM of each), ovalbumin and conalbumin synthesis was increased, when compared to control cultures without hormones, or to oviduct fibroblasts. Progesterone 91-103 ovalbumin (SERPINB14) Gallus gallus 130-139 4026804-0 1985 Decreased ovalbumin-gene response to oestrogen in the prenatally diethylstilboestrol-exposed chick oviduct. diethylstilboestrol 65-84 ovalbumin (SERPINB14) Gallus gallus 10-19 4026804-1 1985 Prenatal exposure of the female chick-embryo Mullerian duct to diethylstilboestrol (DES) decreases its future capacity for epithelial tubular-gland-cell differentiation and oviduct ovalbumin-gene expression. diethylstilboestrol 63-82 ovalbumin (SERPINB14) Gallus gallus 181-190 4026804-1 1985 Prenatal exposure of the female chick-embryo Mullerian duct to diethylstilboestrol (DES) decreases its future capacity for epithelial tubular-gland-cell differentiation and oviduct ovalbumin-gene expression. desacetyluvaricin 84-87 ovalbumin (SERPINB14) Gallus gallus 181-190 4026804-4 1985 Comparisons among these three parameters revealed that the expression of the ovalbumin gene was affected most by DES exposure. desacetyluvaricin 113-116 ovalbumin (SERPINB14) Gallus gallus 77-86 4026804-5 1985 Exposure to high doses of DES suppressed ovalbumin-gene expression by 75-78%, and inhibited tubular-gland-cell differentiation and thus decreased the DNA content by 29 and 32% respectively. desacetyluvaricin 26-29 ovalbumin (SERPINB14) Gallus gallus 41-50 4026804-6 1985 Exposure to low doses of DES caused suppression of ovalbumin-gene expression by 47-53%, but it did not affect the other two parameters. desacetyluvaricin 25-28 ovalbumin (SERPINB14) Gallus gallus 51-60 4016253-2 1985 Guinea-pigs which fasted for 96, 48 and 12 (control) hours were fed intragastrically 300 mg chick ovalbumin in saline, and after 3 hours immunoreactive OA was measured in blood serum by competitive radioimmunoassay. Sodium Chloride 111-117 ovalbumin (SERPINB14) Gallus gallus 98-107 3987617-3 1985 Ovalbumin accounted for 25% of oviduct protein synthesis in chicks treated with low dose of diethylstilbestrol (DES; 0.5 mg/day) for 14 days. Diethylstilbestrol 92-110 ovalbumin (SERPINB14) Gallus gallus 0-9 3987617-3 1985 Ovalbumin accounted for 25% of oviduct protein synthesis in chicks treated with low dose of diethylstilbestrol (DES; 0.5 mg/day) for 14 days. Diethylstilbestrol 112-115 ovalbumin (SERPINB14) Gallus gallus 0-9 3987617-9 1985 Ovalbumin synthesis fluctuated, but overall decreased from 25% of oviduct protein synthesis after priming with DES pellets to 16% of oviduct protein synthesis after 7-12 days of injections of estradiol benzoate (1 mg/day). estradiol 3-benzoate 192-210 ovalbumin (SERPINB14) Gallus gallus 0-9 3937332-3 1985 Significant differences in BSA and OVA digestion by the gastric juice--FGS system were detected both with respect to amino nitrogen content and to the degree of their antigenic structure decomposition, whereas no such differences were observed when the four-enzymic system was used. hydrazine 117-131 ovalbumin (SERPINB14) Gallus gallus 35-38 3996320-2 1985 The simultaneous injection of equal doses of DEX and progesterone resulted in an additive effect on the relative rate of ovalbumin synthesis at all doses tested (range: 0.05-15 mg/chick), even when the induction of ovalbumin synthesis was maximal at 6 h, for either hormone injected alone. Dexamethasone 45-48 ovalbumin (SERPINB14) Gallus gallus 121-130 3996320-2 1985 The simultaneous injection of equal doses of DEX and progesterone resulted in an additive effect on the relative rate of ovalbumin synthesis at all doses tested (range: 0.05-15 mg/chick), even when the induction of ovalbumin synthesis was maximal at 6 h, for either hormone injected alone. Dexamethasone 45-48 ovalbumin (SERPINB14) Gallus gallus 215-224 3996320-2 1985 The simultaneous injection of equal doses of DEX and progesterone resulted in an additive effect on the relative rate of ovalbumin synthesis at all doses tested (range: 0.05-15 mg/chick), even when the induction of ovalbumin synthesis was maximal at 6 h, for either hormone injected alone. Progesterone 53-65 ovalbumin (SERPINB14) Gallus gallus 121-130 3996320-2 1985 The simultaneous injection of equal doses of DEX and progesterone resulted in an additive effect on the relative rate of ovalbumin synthesis at all doses tested (range: 0.05-15 mg/chick), even when the induction of ovalbumin synthesis was maximal at 6 h, for either hormone injected alone. Progesterone 53-65 ovalbumin (SERPINB14) Gallus gallus 215-224 3996320-3 1985 Moreover, the simultaneous injection of DEX and progesterone yielded an additive effect on the relative rates of ovalbumin and conalbumin gene transcription. Dexamethasone 40-43 ovalbumin (SERPINB14) Gallus gallus 113-122 3996320-3 1985 Moreover, the simultaneous injection of DEX and progesterone yielded an additive effect on the relative rates of ovalbumin and conalbumin gene transcription. Progesterone 48-60 ovalbumin (SERPINB14) Gallus gallus 113-122 3996320-5 1985 The simultaneous injection of tamoxifen and DEX potentiated the effect of DEX on the relative rates of ovalbumin and conalbumin synthesis, and amplified the DEX-induced increase in the relative rates of ovalbumin and conalbumin gene transcription. Tamoxifen 30-39 ovalbumin (SERPINB14) Gallus gallus 103-112 3996320-5 1985 The simultaneous injection of tamoxifen and DEX potentiated the effect of DEX on the relative rates of ovalbumin and conalbumin synthesis, and amplified the DEX-induced increase in the relative rates of ovalbumin and conalbumin gene transcription. Tamoxifen 30-39 ovalbumin (SERPINB14) Gallus gallus 203-212 3996320-5 1985 The simultaneous injection of tamoxifen and DEX potentiated the effect of DEX on the relative rates of ovalbumin and conalbumin synthesis, and amplified the DEX-induced increase in the relative rates of ovalbumin and conalbumin gene transcription. Dexamethasone 44-47 ovalbumin (SERPINB14) Gallus gallus 103-112 3996320-5 1985 The simultaneous injection of tamoxifen and DEX potentiated the effect of DEX on the relative rates of ovalbumin and conalbumin synthesis, and amplified the DEX-induced increase in the relative rates of ovalbumin and conalbumin gene transcription. Dexamethasone 44-47 ovalbumin (SERPINB14) Gallus gallus 203-212 3996320-5 1985 The simultaneous injection of tamoxifen and DEX potentiated the effect of DEX on the relative rates of ovalbumin and conalbumin synthesis, and amplified the DEX-induced increase in the relative rates of ovalbumin and conalbumin gene transcription. Dexamethasone 74-77 ovalbumin (SERPINB14) Gallus gallus 103-112 3996320-5 1985 The simultaneous injection of tamoxifen and DEX potentiated the effect of DEX on the relative rates of ovalbumin and conalbumin synthesis, and amplified the DEX-induced increase in the relative rates of ovalbumin and conalbumin gene transcription. Dexamethasone 74-77 ovalbumin (SERPINB14) Gallus gallus 103-112 2580958-2 1985 The immunogenic hexapeptide H-Gly-Arg-Gly-Thr-Lys-Phe-OH was coupled to chicken egg-albumin with dimethylsuberimidate. H-Gly-Arg-Gly-Thr-Lys-Phe-OH 28-56 ovalbumin (SERPINB14) Gallus gallus 80-91 2580958-2 1985 The immunogenic hexapeptide H-Gly-Arg-Gly-Thr-Lys-Phe-OH was coupled to chicken egg-albumin with dimethylsuberimidate. Dimethyl Suberimidate 97-117 ovalbumin (SERPINB14) Gallus gallus 80-91 3880544-1 1985 A primary cell culture from estrogen-withdrawn chicken oviduct has been developed in which the genes for the major egg white proteins, ovalbumin and conalbumin, are induced by steroid hormones. Steroids 176-192 ovalbumin (SERPINB14) Gallus gallus 135-144 4000950-7 1985 Whereas 40-45% of the beta A chromatin is recovered in the supernatant fraction from n-butyrate incubated immature erythrocytes, nucleohistone containing ovalbumin DNA sequences remains insoluble. nucleohistone 129-142 ovalbumin (SERPINB14) Gallus gallus 154-163 4005225-5 1985 After its administration together with or 6 h after diethylstilbestrol (a synthetic estrogen), tamoxifen stopped or suppressed the estrogen-dependent increase of ovalbumin and conalbumin gene transcription. Tamoxifen 95-104 ovalbumin (SERPINB14) Gallus gallus 162-171 3880544-4 1985 Both insulin and a second steroid (a glucocorticoid, progestin, or androgen) are required for the induction of the ovalbumin gene by estradiol to the extent reached in vivo. Steroids 26-33 ovalbumin (SERPINB14) Gallus gallus 115-124 3880544-4 1985 Both insulin and a second steroid (a glucocorticoid, progestin, or androgen) are required for the induction of the ovalbumin gene by estradiol to the extent reached in vivo. Estradiol 133-142 ovalbumin (SERPINB14) Gallus gallus 115-124 3880544-7 1985 The data obtained with the cultured cells demonstrate that the induction of the ovalbumin gene requires the permissive effects of insulin and a second steroid (probably a glucocorticoid in vivo) to facilitate the response to estradiol. Steroids 151-158 ovalbumin (SERPINB14) Gallus gallus 80-89 3880544-7 1985 The data obtained with the cultured cells demonstrate that the induction of the ovalbumin gene requires the permissive effects of insulin and a second steroid (probably a glucocorticoid in vivo) to facilitate the response to estradiol. Estradiol 225-234 ovalbumin (SERPINB14) Gallus gallus 80-89 6329659-0 1984 Regulation of the ovalbumin gene: effects of insulin, adenosine 3",5"-monophosphate, and estrogen. Cyclic AMP 54-83 ovalbumin (SERPINB14) Gallus gallus 18-27 6332146-3 1984 A 17-amino acid tryptic peptide of chicken ovalbumin, designated P323-339, that substituted for processed antigen when presented by glutaraldehyde prefixed accessory cells to specific I-restricted T hybridomas was characterized. 17-amino acid 2-15 ovalbumin (SERPINB14) Gallus gallus 43-52 6332146-3 1984 A 17-amino acid tryptic peptide of chicken ovalbumin, designated P323-339, that substituted for processed antigen when presented by glutaraldehyde prefixed accessory cells to specific I-restricted T hybridomas was characterized. Peptides 24-31 ovalbumin (SERPINB14) Gallus gallus 43-52 6332146-3 1984 A 17-amino acid tryptic peptide of chicken ovalbumin, designated P323-339, that substituted for processed antigen when presented by glutaraldehyde prefixed accessory cells to specific I-restricted T hybridomas was characterized. Glutaral 132-146 ovalbumin (SERPINB14) Gallus gallus 43-52 6329659-7 1984 We found that cyclic nucleotide derivatives, such as 8-bromo-cAMP, can mimic the effect of insulin on ovalbumin gene expression and that the phosphodiesterase inhibitor isobutylmethylxanthine can potentiate this response to 8-bromo-cAMP. Nucleotides, Cyclic 14-31 ovalbumin (SERPINB14) Gallus gallus 102-111 6329659-7 1984 We found that cyclic nucleotide derivatives, such as 8-bromo-cAMP, can mimic the effect of insulin on ovalbumin gene expression and that the phosphodiesterase inhibitor isobutylmethylxanthine can potentiate this response to 8-bromo-cAMP. 8-Bromo Cyclic Adenosine Monophosphate 53-65 ovalbumin (SERPINB14) Gallus gallus 102-111 6329659-7 1984 We found that cyclic nucleotide derivatives, such as 8-bromo-cAMP, can mimic the effect of insulin on ovalbumin gene expression and that the phosphodiesterase inhibitor isobutylmethylxanthine can potentiate this response to 8-bromo-cAMP. 1-Methyl-3-isobutylxanthine 169-191 ovalbumin (SERPINB14) Gallus gallus 102-111 6329659-7 1984 We found that cyclic nucleotide derivatives, such as 8-bromo-cAMP, can mimic the effect of insulin on ovalbumin gene expression and that the phosphodiesterase inhibitor isobutylmethylxanthine can potentiate this response to 8-bromo-cAMP. 8-Bromo Cyclic Adenosine Monophosphate 224-236 ovalbumin (SERPINB14) Gallus gallus 102-111 6329659-10 1984 We conclude that insulin and cAMP are producing their effects on activation of the ovalbumin gene via convergent pathways. Cyclic AMP 29-33 ovalbumin (SERPINB14) Gallus gallus 83-92 6329659-11 1984 The involvement of an activator of protein kinase, cAMP, strongly suggests that protein phosphorylation events play a regulatory role in the expression of the ovalbumin gene. Cyclic AMP 51-55 ovalbumin (SERPINB14) Gallus gallus 159-168 6646237-1 1983 In the chicken oviduct, it has been well documented that steroid hormones stimulate the transcription of specific genes such as the ovalbumin gene. Steroids 57-73 ovalbumin (SERPINB14) Gallus gallus 132-141 6313672-1 1983 To study the regulation of expression of the chicken ovalbumin gene by steroid hormones, the entire ovalbumin gene and its flanking sequences were cloned together with the bacterial gene for xanthine-guanine phosphoribosyltransferase in plasmid pBR322. Steroids 71-87 ovalbumin (SERPINB14) Gallus gallus 53-62 6313672-6 1983 An 8- to 10-fold increase in the amount of ovalbumin mRNA was observed to be present in cells cultured in 10(-8)M estradiol. Estradiol 114-123 ovalbumin (SERPINB14) Gallus gallus 43-52 6314276-8 1983 When recombinants are introduced into eukaryotic cells by either calcium phosphate coprecipitation or protoplast fusion, expression of chicken ovalbumin or conalbumin may be detected by indirect immunofluorescence. calcium phosphate 65-82 ovalbumin (SERPINB14) Gallus gallus 143-152 6621702-1 1983 Ovalbumin gene transcripts are not detectable in unstimulated chick oviducts but comprise about half of oviduct cell transcripts after steroid hormone induction. Steroids 135-150 ovalbumin (SERPINB14) Gallus gallus 0-9 7160465-0 1982 The effect of estrogen on the concentration of ovalbumin gene sequence in the 2 M NaCl residual fraction of oviduct chromatin. Sodium Chloride 82-86 ovalbumin (SERPINB14) Gallus gallus 47-56 6571991-3 1983 About a 10-fold preference was seen for DNA fragments flanking the 5" end of the steroid-regulated genes ovalbumin and gene Y. Steroids 81-88 ovalbumin (SERPINB14) Gallus gallus 105-114 6557011-1 1983 The ovalbumin gene and the ovalbumin-related X and Y genes are expressed in the chicken oviduct in response to steroid hormones. Steroids 111-127 ovalbumin (SERPINB14) Gallus gallus 4-13 6557011-1 1983 The ovalbumin gene and the ovalbumin-related X and Y genes are expressed in the chicken oviduct in response to steroid hormones. Steroids 111-127 ovalbumin (SERPINB14) Gallus gallus 27-36 7130193-1 1982 We have examined the effects of steroid hormones in the chromatin sensitivity of the ovalbumin gene to micrococcal nuclease and have attempted to define the importance of the nucleosome core, higher order chromatin folding, and transcription in the maintenance of the nuclease-sensitive conformation of the ovalbumin chromatin. Steroids 32-39 ovalbumin (SERPINB14) Gallus gallus 85-94 7084121-2 1982 In estrogen differentiated, hormone-withdrawn animals, biochemical and ultrastructural data indicated that the combined treatment with progesterone plus tamoxifen increased conalbumin (+100%) and ovalbumin (+30%) more than progesterone alone and displayed estrogen-like growth-promoting properties (+50% in DNA content per oviduct). Progesterone 135-147 ovalbumin (SERPINB14) Gallus gallus 196-205 7171239-2 1982 In estrogen differentiated, hormone withdrawn animals, biochemical and ultrastructural data indicated that the combined treatment with progesterone plus tamoxifen increased conalbumin (+ 100%) and ovalbumin (+ 30%) more than progesterone alone, and displayed estrogen-like growth promoting properties (+ 50% in DNA content/oviduct). Progesterone 135-147 ovalbumin (SERPINB14) Gallus gallus 173-206 7171239-2 1982 In estrogen differentiated, hormone withdrawn animals, biochemical and ultrastructural data indicated that the combined treatment with progesterone plus tamoxifen increased conalbumin (+ 100%) and ovalbumin (+ 30%) more than progesterone alone, and displayed estrogen-like growth promoting properties (+ 50% in DNA content/oviduct). Tamoxifen 153-162 ovalbumin (SERPINB14) Gallus gallus 173-206 7084121-2 1982 In estrogen differentiated, hormone-withdrawn animals, biochemical and ultrastructural data indicated that the combined treatment with progesterone plus tamoxifen increased conalbumin (+100%) and ovalbumin (+30%) more than progesterone alone and displayed estrogen-like growth-promoting properties (+50% in DNA content per oviduct). Tamoxifen 153-162 ovalbumin (SERPINB14) Gallus gallus 196-205 7263632-0 1981 Steroid hormone regulation of ovalbumin and conalbumin gene transcription. Steroids 0-15 ovalbumin (SERPINB14) Gallus gallus 30-39 6272839-8 1981 Similarly, the hexanucleotide sequence AATAAA common to all eucaryotic messenger RNAs at the 3"-untranslated region occurs seven additional times within the ovalbumin gene. hexanucleotide 15-29 ovalbumin (SERPINB14) Gallus gallus 157-166 7263632-6 1981 These ideas are combined with our recent observations that protein synthesis inhibitors and butyrate selectively, but reversibly, inhibit ovalbumin and conalbumin gene transcription. Butyrates 92-100 ovalbumin (SERPINB14) Gallus gallus 138-147 6257282-7 1980 R0t analysis has demonstrated that, similar to the ovalbumin gene, the level of X and Y gene transcripts is increased by the steroid hormone estrogen, but to varying degrees. Steroids 125-140 ovalbumin (SERPINB14) Gallus gallus 51-60 6162638-4 1981 However, ovalbumin, which is transferred across the endoplasmic reticulum in the presence of tunicamycin and which is indistinguishable by immunoprecipitation, by two-dimensional gel electrophoresis and by concanavalin-A--Sepharose binding from the cytosolic form, is still secreted. Tunicamycin 93-104 ovalbumin (SERPINB14) Gallus gallus 9-18 6162638-4 1981 However, ovalbumin, which is transferred across the endoplasmic reticulum in the presence of tunicamycin and which is indistinguishable by immunoprecipitation, by two-dimensional gel electrophoresis and by concanavalin-A--Sepharose binding from the cytosolic form, is still secreted. Sepharose 222-231 ovalbumin (SERPINB14) Gallus gallus 9-18 7284304-0 1981 Demonstration of heterogeneity of chick ovalbumin glycopeptides using 360-MHz proton magnetic resonance spectroscopy. Glycopeptides 50-63 ovalbumin (SERPINB14) Gallus gallus 40-49 7278996-2 1981 In the chicken oviduct the synthesis of the egg-white proteins ovalbumin, conalbumin, ovomucoid and lysozyme is controlled by the female sex steroids. Steroids 141-149 ovalbumin (SERPINB14) Gallus gallus 63-72 7448870-1 1980 The short chain aliphatic acid salts, butyrate and propionate, are effective inhibitors of histone deacetylation in chick oviduct at 2--5 mM; they also prevent the hormonal induction of the ovalbumin and transferrin genes. aliphatic acid salts 16-36 ovalbumin (SERPINB14) Gallus gallus 190-199 7448870-1 1980 The short chain aliphatic acid salts, butyrate and propionate, are effective inhibitors of histone deacetylation in chick oviduct at 2--5 mM; they also prevent the hormonal induction of the ovalbumin and transferrin genes. Butyrates 38-46 ovalbumin (SERPINB14) Gallus gallus 190-199 7448870-1 1980 The short chain aliphatic acid salts, butyrate and propionate, are effective inhibitors of histone deacetylation in chick oviduct at 2--5 mM; they also prevent the hormonal induction of the ovalbumin and transferrin genes. Propionates 51-61 ovalbumin (SERPINB14) Gallus gallus 190-199 7448870-4 1980 In addition to preventing the induction, butyrate also causes a rapid deinduction when added to preinduced cultures; ovalbumin and transferrin gene transcription decline with a half-life of 15--30 min. Butyrates 41-49 ovalbumin (SERPINB14) Gallus gallus 117-126 7419588-8 1980 When 3-d-withdrawn oviducts were restimulated with either estrogen or progesterone, in situ hybridization revealed that greater than or equal to 98% of the tubular gland cells contained ovalbumin mRNA. Progesterone 70-82 ovalbumin (SERPINB14) Gallus gallus 186-195 7418002-1 1980 The X, Y and ovalbumin genes, which are found within a 40 kb region of the chicken genome, are all expressed in oviduct under steroid hormone control, and share some sequence homologies. Steroids 126-141 ovalbumin (SERPINB14) Gallus gallus 13-22 6891480-0 1980 [Control mechanisms of ovalbumin and conalbumin synthesis in chicken oviducts by estradiol and progesterone. Estradiol 81-90 ovalbumin (SERPINB14) Gallus gallus 23-32 7387999-0 1980 Ovalbumin messenger ribonucleic acid accumulation in the chick oviduct during secondary stimulation: influence of combinations of steroid hormones and circannual rhythms. Steroids 130-146 ovalbumin (SERPINB14) Gallus gallus 0-9 7387999-1 1980 We have analyzed by hybridization the accumulation of ovalbumin mRNA after the administration of estrogen, progesterone, these two hormones together, or each hormone with testosterone to "withdrawn" chicks (chicks previously stimulated with estrogen but then withdrawn from the hormone). Progesterone 107-119 ovalbumin (SERPINB14) Gallus gallus 54-63 7398635-0 1980 Effect of tamoxifen on oestradiol and progesterone-induced synthesis of ovalbumin and conalbumin in chick oviduct. Tamoxifen 10-19 ovalbumin (SERPINB14) Gallus gallus 72-81 7398635-0 1980 Effect of tamoxifen on oestradiol and progesterone-induced synthesis of ovalbumin and conalbumin in chick oviduct. Progesterone 38-50 ovalbumin (SERPINB14) Gallus gallus 72-81 6891480-0 1980 [Control mechanisms of ovalbumin and conalbumin synthesis in chicken oviducts by estradiol and progesterone. Progesterone 95-107 ovalbumin (SERPINB14) Gallus gallus 23-32 508284-5 1979 The results were correlated with diethylstilboestrol-induced ovalbumin-gene expression as measured by ovalbumin-mRNA (mRNAov) accumulation and the relative rate of ovalbumin synthesis. diethylstilboestrol 33-52 ovalbumin (SERPINB14) Gallus gallus 61-70 479179-0 1979 Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct. Steroids 68-84 ovalbumin (SERPINB14) Gallus gallus 34-43 519756-8 1979 Thirty nucleotides before the start of the messenger RNA coding sequence is the heptanucleotide TATATAT, which is also present in a similar location relative to the chicken ovalbumin gene and other unique sequence eucaryotic genes. heptanucleotide 80-95 ovalbumin (SERPINB14) Gallus gallus 173-182 508284-5 1979 The results were correlated with diethylstilboestrol-induced ovalbumin-gene expression as measured by ovalbumin-mRNA (mRNAov) accumulation and the relative rate of ovalbumin synthesis. diethylstilboestrol 33-52 ovalbumin (SERPINB14) Gallus gallus 102-111 508284-5 1979 The results were correlated with diethylstilboestrol-induced ovalbumin-gene expression as measured by ovalbumin-mRNA (mRNAov) accumulation and the relative rate of ovalbumin synthesis. diethylstilboestrol 33-52 ovalbumin (SERPINB14) Gallus gallus 102-111 104296-2 1978 When a plasmid containing the hybrid gene was introduced into E. coli, a protein identified as ovalbumin by immunoreactivity and sodium dodecyl sulfate/polyacrylamide gel electrophoresis was synthesized. Sodium Dodecyl Sulfate 129-151 ovalbumin (SERPINB14) Gallus gallus 95-104 286292-3 1979 Of the RNA synthesized by oviduct nuclei from chickens chronically stimulated with diethylstilbestrol, 0.23% corresponded to ovalbumin mRNA and 0.17% were transcripts of intervening sequences. Diethylstilbestrol 83-101 ovalbumin (SERPINB14) Gallus gallus 125-134 286292-8 1979 Previously we had identified multiple species of putative precursors of ovalbumin mRNA in oviduct nuclei from chickens chronically stimulated with diethylstilbestrol. Diethylstilbestrol 147-165 ovalbumin (SERPINB14) Gallus gallus 72-81 286292-9 1979 We demonstrate here that withdrawal of diethylstilbestrol resulted in a depletion of high-molecular-weight ovalbumin RNA and of mature ovalbumin mRNA and that readministration of the estrogen induced the nuclear accumulation of both forms of ovalbumin RNA. Diethylstilbestrol 39-57 ovalbumin (SERPINB14) Gallus gallus 107-116 286292-9 1979 We demonstrate here that withdrawal of diethylstilbestrol resulted in a depletion of high-molecular-weight ovalbumin RNA and of mature ovalbumin mRNA and that readministration of the estrogen induced the nuclear accumulation of both forms of ovalbumin RNA. Diethylstilbestrol 39-57 ovalbumin (SERPINB14) Gallus gallus 135-144 286292-9 1979 We demonstrate here that withdrawal of diethylstilbestrol resulted in a depletion of high-molecular-weight ovalbumin RNA and of mature ovalbumin mRNA and that readministration of the estrogen induced the nuclear accumulation of both forms of ovalbumin RNA. Diethylstilbestrol 39-57 ovalbumin (SERPINB14) Gallus gallus 135-144 104296-2 1978 When a plasmid containing the hybrid gene was introduced into E. coli, a protein identified as ovalbumin by immunoreactivity and sodium dodecyl sulfate/polyacrylamide gel electrophoresis was synthesized. polyacrylamide 152-166 ovalbumin (SERPINB14) Gallus gallus 95-104 78523-1 1978 Kepone induces ovalbumin and conalbumin synthesis in explants of chick oviduct in vitro by acting as a weak estrogen. Chlordecone 0-6 ovalbumin (SERPINB14) Gallus gallus 15-24 358194-5 1978 Five out of approximately 20,000 recombinant phage plaques were capable of hybridizing with a (32)P-labeled Hha I fragment of a recombinant plasmid pOV230 containing the entire structural ovalbumin gene. Phosphorus-32 94-99 ovalbumin (SERPINB14) Gallus gallus 188-197 273231-6 1978 The synthesis of ovalbumin RNA progressed during the incubation of nuclei and was sensitive to actinomycin D and low concentrations of alpha-amanitin. Dactinomycin 95-108 ovalbumin (SERPINB14) Gallus gallus 17-26 661981-1 1978 The complete sequence of chicken ovalbumin mRNA is presented; it is 1,859 residues long, excluding its terminal "cap" and poly(A). Poly A 122-129 ovalbumin (SERPINB14) Gallus gallus 33-42 273231-6 1978 The synthesis of ovalbumin RNA progressed during the incubation of nuclei and was sensitive to actinomycin D and low concentrations of alpha-amanitin. Alpha-Amanitin 135-149 ovalbumin (SERPINB14) Gallus gallus 17-26 845159-6 1977 The concentration of nuclear estrogen receptors achieved by administering different dosages of 17beta-estradiol, 17beta-estradiol-benzoate, or diethylstilbestrol is related to the rate of accumulation of ovalbumin and conalbumin mRNA. Estradiol 95-111 ovalbumin (SERPINB14) Gallus gallus 205-214 563592-2 1977 Hybridisation with terminally 32P-labelled ovalbumin mRNA fragments or with RNA populations transcribed from the DNA of a hybrid plasmid containing ovalbumin sequences was used to locate the DNA fragments coding for ovalbumin. Phosphorus-32 30-33 ovalbumin (SERPINB14) Gallus gallus 43-52 318336-9 1978 The carbohydrate compositions were slightly higher in hexose and lower in hexosamine than chicken ovalbumin. Carbohydrates 4-16 ovalbumin (SERPINB14) Gallus gallus 98-107 845159-6 1977 The concentration of nuclear estrogen receptors achieved by administering different dosages of 17beta-estradiol, 17beta-estradiol-benzoate, or diethylstilbestrol is related to the rate of accumulation of ovalbumin and conalbumin mRNA. estradiol-17 beta-benzoate 113-138 ovalbumin (SERPINB14) Gallus gallus 205-214 845159-6 1977 The concentration of nuclear estrogen receptors achieved by administering different dosages of 17beta-estradiol, 17beta-estradiol-benzoate, or diethylstilbestrol is related to the rate of accumulation of ovalbumin and conalbumin mRNA. Diethylstilbestrol 143-161 ovalbumin (SERPINB14) Gallus gallus 205-214 55272-6 1976 When such a complete ovalbumin [3H]cDNA was synthesized with a specific activity of 10(8) cpm/mug and hyfridized to an excess of chick DNA, the kinetics of hybridization indicated that the cDNA was comprised of a nonrepetitive sequence. Tritium 32-34 ovalbumin (SERPINB14) Gallus gallus 21-30 941868-8 1976 Substitution of beef, lamb, pork, liver, fish, and chicken for the egg ovalbumin in the sannisynthetic meal resulted in a significant, 2-fold to 4-fold increase in iron absorption whereas no increase was observed with milk, cheese, or egg. Iron 164-168 ovalbumin (SERPINB14) Gallus gallus 71-80 932010-3 1976 The coding strand of the ovalbumin gene was partially purified from sheared chick DNA by affinity column chromatography using ovalbumin mRNA immobilized on phosphocellulose. phosphocellulose 156-172 ovalbumin (SERPINB14) Gallus gallus 25-34 932010-4 1976 The concentrations of the ovalbumin DNA sequence in various DNA fractions were quantitated by measuring their rates of hybridization with 125I-labeled ovalbumin mRNA. Iodine-125 138-142 ovalbumin (SERPINB14) Gallus gallus 26-35 932010-4 1976 The concentrations of the ovalbumin DNA sequence in various DNA fractions were quantitated by measuring their rates of hybridization with 125I-labeled ovalbumin mRNA. Iodine-125 138-142 ovalbumin (SERPINB14) Gallus gallus 151-160 987847-10 1976 Ethionine induces ovalbumin and conalbumin synthesis in immature chick oviducts that were withdrawn from estrogen for 3 to 4 weeks following primary estrogen stimulation. Ethionine 0-9 ovalbumin (SERPINB14) Gallus gallus 18-27 182385-0 1976 A significant lag in the induction of ovalbumin messenger RNA by steroid hormones: a receptor translocation hypothesis. Steroids 65-81 ovalbumin (SERPINB14) Gallus gallus 38-47 182385-7 1976 Treatment of chicks with hydroxyurea shortens the lag for ovalbumin induction with either hormone. Hydroxyurea 25-36 ovalbumin (SERPINB14) Gallus gallus 58-67 182385-9 1976 With cycloheximide, however, ovalbumin mRNA accumulation can be prevented. Cycloheximide 5-18 ovalbumin (SERPINB14) Gallus gallus 29-38 1250407-0 1976 Induction of ovalbumin and conalbumin synthesis in immature chick oviducts by ethionine. Ethionine 78-87 ovalbumin (SERPINB14) Gallus gallus 13-22 1176463-1 1975 A complementary DNA synthesized from ovalbumin mRNA was used in hybridization experiments to study the early effect of estrogen and progesterone on the accumulation of ovalbumin mRNA sequences in the chick oviduct. Progesterone 132-144 ovalbumin (SERPINB14) Gallus gallus 168-177 4329151-5 1971 Ovalbumin is undetectable in the unstimulated chick oviduct and in oviducts of chicks treated with progesterone (P) for up to 5 days. Progesterone 99-111 ovalbumin (SERPINB14) Gallus gallus 0-9 1158896-1 1975 Preparative agarose gel electrophoresis under denaturing conditions has been successfully employed to purify large quantities of ovalbumin mRNA from hen oviducts. Sepharose 12-19 ovalbumin (SERPINB14) Gallus gallus 129-138 1158896-4 1975 It is also not contaminated by other biologically active messenger RNAs because, when it is added to the cell-free wheat germ translation system, the only protein product synthesized is ovalbumin as analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and specific immunoprecipitation. polyacrylamide 211-225 ovalbumin (SERPINB14) Gallus gallus 186-195 4531006-4 1974 Furthermore, poly(A)-free globin mRNA competed with the same efficiency as authentic globin mRNA against chick ovalbumin mRNA when translated under total mRNA saturation conditions. Poly A 13-20 ovalbumin (SERPINB14) Gallus gallus 111-120 4129926-0 1973 Synthesis of (3H)DNA complementary to ovalbumin messenger RNA: evidence for limited copies of the ovalbumin gene in chick oviduct. Tritium 14-16 ovalbumin (SERPINB14) Gallus gallus 38-47 4515943-0 1973 Rates of induction of specific translatable messenger RNAs for ovalbumin and avidin by steroid hormones. Steroids 87-103 ovalbumin (SERPINB14) Gallus gallus 63-72 4515943-1 1973 In the chick oviduct, injections of estrogen and progesterone induce synthesis of the specific proteins ovalbumin and avidin, respectively. Progesterone 49-61 ovalbumin (SERPINB14) Gallus gallus 104-113 4515943-4 1973 Single injections of estrogen in chicks previously withdrawn from all steroid hormones for 2 weeks led to rapid increases in ovalbumin mRNA with 3 hr, which coincided with increases in the rate of ovalbumin synthesis. Steroids 70-86 ovalbumin (SERPINB14) Gallus gallus 125-134 5542686-0 1971 Modulation of ovalbumin synthesis by estradiol-17 beta and actinomycin D as studied in explants of chick oviduct in culture. Estradiol 37-54 ovalbumin (SERPINB14) Gallus gallus 14-23 5542686-0 1971 Modulation of ovalbumin synthesis by estradiol-17 beta and actinomycin D as studied in explants of chick oviduct in culture. Dactinomycin 59-72 ovalbumin (SERPINB14) Gallus gallus 14-23 5350180-4 1969 Such function, as measured by the increase in specific cell products such as lysozyme and ovalbumin, requires the continuous presence of estrogen or progesterone. Progesterone 149-161 ovalbumin (SERPINB14) Gallus gallus 90-99 5814004-4 1969 Progesterone administered concomitantly with estrogen antagonizes the estrogen-induced tissue growth as well as appearance of tubular gland cells and their specific products, lysozyme and ovalbumin. Progesterone 0-12 ovalbumin (SERPINB14) Gallus gallus 188-197 5814004-7 1969 The above results of progesterone antagonism can best be explained by the hypothesis that progesterone inhibits the initial proliferation of cells which become tubular gland cells but does not antagonize the subsequent cytodifferentiation leading to the synthesis of lysozyme and ovalbumin once such cell proliferation has occurred. Progesterone 21-33 ovalbumin (SERPINB14) Gallus gallus 280-289 4694721-2 1973 Mechanism of ovalbumin "superinduction" by actinomycin D. Dactinomycin 43-56 ovalbumin (SERPINB14) Gallus gallus 13-22 4114403-0 1972 Investigation of the antigenicity of the carbohydrate moiety of chicken ovalbumin. Carbohydrates 41-53 ovalbumin (SERPINB14) Gallus gallus 72-81 6007449-37 1966 After short periods of incubation of minced tissue with [(14)C]lysine some of the radioactive protein of the isolated I particles behaved as ovalbumin. [(14)c]lysine 56-69 ovalbumin (SERPINB14) Gallus gallus 141-150 4329151-8 1971 When E + P are administered together there is initially a synergistic effect on ovalbumin synthesis, however, after 2 days ovalbumin synthesis slows and by 5 days there is only 1/20th as much ovalbumin per magnum as in the E-treated controls. e + p 5-10 ovalbumin (SERPINB14) Gallus gallus 80-89 32729901-4 2021 Using a doxycycline-driven gene expression system, we generated murine MC38, CT26 (colorectal cancer) and B16 (melanoma) cell lines with inducible expression of model immunogenic neoantigens such as chicken ovalbumin and human NY-ESO-1. Doxycycline 8-19 ovalbumin (SERPINB14) Gallus gallus 207-216 33519467-0 2020 3-Hydroxyphthalic Anhydride-Modified Chicken Ovalbumin as a Potential Candidate Inhibits SARS-CoV-2 Infection by Disrupting the Interaction of Spike Protein With Host ACE2 Receptor. 3-hydroxyphthalic anhydride 0-27 ovalbumin (SERPINB14) Gallus gallus 45-54 33519467-3 2020 Our previous studies have reported that 3-hydroxyphthalic anhydride-modified chicken ovalbumin (HP-OVA) serves as a viral entry inhibitor to prevent several kinds of virus infection. 3-hydroxyphthalic anhydride 40-67 ovalbumin (SERPINB14) Gallus gallus 85-94 33096433-0 2021 A broad-spectrum sensing strategy for the tetracycline family of antibiotics based on an ovalbumin-stabilized gold nanocluster and its application in a pump-free microfluidic sensing platform. Tetracycline 42-54 ovalbumin (SERPINB14) Gallus gallus 89-98 33096433-3 2021 The OVA-stabilized AuNCs (AuNCs@OVA) manifest intriguing multicolour fluorescence and a gradually declining fluorescence intensity at 650 nm with an increasing concentration of tetracycline family antibiotics (TCs) including tetracycline, chlorotetracycline, oxytetracycline, and doxycycline, which are a widely used class of antibiotics for treating infections in food-producing animals. tetracycline family antibiotics 177-208 ovalbumin (SERPINB14) Gallus gallus 4-7 33096433-3 2021 The OVA-stabilized AuNCs (AuNCs@OVA) manifest intriguing multicolour fluorescence and a gradually declining fluorescence intensity at 650 nm with an increasing concentration of tetracycline family antibiotics (TCs) including tetracycline, chlorotetracycline, oxytetracycline, and doxycycline, which are a widely used class of antibiotics for treating infections in food-producing animals. 9-ethyl-N-(3,4,5-trimethoxyphenyl)carbazole-3-sulfonamide 210-213 ovalbumin (SERPINB14) Gallus gallus 4-7 33096433-3 2021 The OVA-stabilized AuNCs (AuNCs@OVA) manifest intriguing multicolour fluorescence and a gradually declining fluorescence intensity at 650 nm with an increasing concentration of tetracycline family antibiotics (TCs) including tetracycline, chlorotetracycline, oxytetracycline, and doxycycline, which are a widely used class of antibiotics for treating infections in food-producing animals. Tetracycline 177-189 ovalbumin (SERPINB14) Gallus gallus 4-7 33096433-3 2021 The OVA-stabilized AuNCs (AuNCs@OVA) manifest intriguing multicolour fluorescence and a gradually declining fluorescence intensity at 650 nm with an increasing concentration of tetracycline family antibiotics (TCs) including tetracycline, chlorotetracycline, oxytetracycline, and doxycycline, which are a widely used class of antibiotics for treating infections in food-producing animals. Chlortetracycline 239-257 ovalbumin (SERPINB14) Gallus gallus 4-7 33096433-3 2021 The OVA-stabilized AuNCs (AuNCs@OVA) manifest intriguing multicolour fluorescence and a gradually declining fluorescence intensity at 650 nm with an increasing concentration of tetracycline family antibiotics (TCs) including tetracycline, chlorotetracycline, oxytetracycline, and doxycycline, which are a widely used class of antibiotics for treating infections in food-producing animals. Oxytetracycline 259-274 ovalbumin (SERPINB14) Gallus gallus 4-7 33096433-3 2021 The OVA-stabilized AuNCs (AuNCs@OVA) manifest intriguing multicolour fluorescence and a gradually declining fluorescence intensity at 650 nm with an increasing concentration of tetracycline family antibiotics (TCs) including tetracycline, chlorotetracycline, oxytetracycline, and doxycycline, which are a widely used class of antibiotics for treating infections in food-producing animals. Doxycycline 280-291 ovalbumin (SERPINB14) Gallus gallus 4-7 33096433-4 2021 This performance makes AuNCs@OVA particularly attractive as a broad-spectrum detector for TCs sensing, and we demonstrate that this simple sensing procedure can be realized in real time by directly mixing the target sample and AuNCs@OVA components. 9-ethyl-N-(3,4,5-trimethoxyphenyl)carbazole-3-sulfonamide 90-93 ovalbumin (SERPINB14) Gallus gallus 29-32 33718858-9 2021 In co-cultures of naive DO11.10 T cells and OVA peptide-loaded antigen-presenting cells (APCs), pre-exposure of the T cells (but not pre-exposure of APCs) to 4MU inhibited early T cell activation (CD69 expression). Hymecromone 158-161 ovalbumin (SERPINB14) Gallus gallus 44-47 31386980-8 2019 Our data suggest that mangiferin exerted anti-asthmatic effect through decreasing Th9 and Th17 responses and increasing Treg response in OVA-induced asthmatic mouse model. mangiferin 22-32 ovalbumin (SERPINB14) Gallus gallus 137-140 32459249-2 2020 Herein, in order to prevent intermolecular accumulation and improve photostability, indocyanine green (ICG) is spontaneously adsorbed onto a covalent organic framework (COF) with high affinity through pi-pi conjugation, and then chicken ovalbumin (OVA) is coated on the surface of COF@ICG via an electrostatic interaction force. Indocyanine Green 84-101 ovalbumin (SERPINB14) Gallus gallus 237-246 32459249-2 2020 Herein, in order to prevent intermolecular accumulation and improve photostability, indocyanine green (ICG) is spontaneously adsorbed onto a covalent organic framework (COF) with high affinity through pi-pi conjugation, and then chicken ovalbumin (OVA) is coated on the surface of COF@ICG via an electrostatic interaction force. Indocyanine Green 84-101 ovalbumin (SERPINB14) Gallus gallus 248-251 32026252-4 2020 Two types of reductive N-glycans were released from chicken egg albumin (ovalbumin) and soy protein using an ammonia catalysis method and labeled with benzenesulfonyl hydrazide (BSH). n-glycans 23-32 ovalbumin (SERPINB14) Gallus gallus 60-71 32026252-4 2020 Two types of reductive N-glycans were released from chicken egg albumin (ovalbumin) and soy protein using an ammonia catalysis method and labeled with benzenesulfonyl hydrazide (BSH). n-glycans 23-32 ovalbumin (SERPINB14) Gallus gallus 73-82 32026252-4 2020 Two types of reductive N-glycans were released from chicken egg albumin (ovalbumin) and soy protein using an ammonia catalysis method and labeled with benzenesulfonyl hydrazide (BSH). benzenesulfohydrazide 178-181 ovalbumin (SERPINB14) Gallus gallus 73-82 32026252-8 2020 A total of 21 and 8 N-glycan-AEAB conjugates were obtained from ovalbumin and soy protein, respectively. n-glycan-aeab 20-33 ovalbumin (SERPINB14) Gallus gallus 64-73 31466032-0 2020 How black tea pigment theaflavin dyes chicken eggs: Binding affinity study of theaflavin with ovalbumin. theaflavin 22-32 ovalbumin (SERPINB14) Gallus gallus 94-103 31466032-0 2020 How black tea pigment theaflavin dyes chicken eggs: Binding affinity study of theaflavin with ovalbumin. theaflavin 78-88 ovalbumin (SERPINB14) Gallus gallus 94-103 32010807-2 2020 The hapten DIG was coupled to bovine serum albumin or chicken ovalbumin by sodium periodate oxidation. metaperiodate 75-91 ovalbumin (SERPINB14) Gallus gallus 62-71 32012983-8 2020 In a murine tolerogenic antigen challenge model, chronic systemic exposure to BPA was sufficient to induce airway sensitization to innocuous chicken egg ovalbumin in the complete absence of adjuvants. bisphenol A 78-81 ovalbumin (SERPINB14) Gallus gallus 153-162 29149729-4 2018 Nevertheless, naturally available glycoproteins, such as ovalbumin in chicken egg whites, are good sources for fabricating glycan-immobilized nanoprobes. Polysaccharides 123-129 ovalbumin (SERPINB14) Gallus gallus 57-66 30184130-0 2019 Large-scale purification of ovalbumin using polyethylene glycol precipitation and isoelectric precipitation. Polyethylene Glycols 44-63 ovalbumin (SERPINB14) Gallus gallus 28-37 30101285-0 2019 Hydroxyl radical-induced early stage oxidation improves the foaming and emulsifying properties of ovalbumin. Hydroxyl Radical 0-16 ovalbumin (SERPINB14) Gallus gallus 98-107 30101285-2 2019 The present study investigated the effects of hydroxyl radical-induced early stage oxidation on the physicochemical and interfacial properties of chicken egg white ovalbumin. Hydroxyl Radical 46-62 ovalbumin (SERPINB14) Gallus gallus 164-173 30101285-4 2019 Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed that the exposure of ovalbumin to hydroxyl radicals caused self-cross-linking and resulted in the formation of dimers and trimers. Sodium Dodecyl Sulfate 0-22 ovalbumin (SERPINB14) Gallus gallus 95-104 30101285-4 2019 Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed that the exposure of ovalbumin to hydroxyl radicals caused self-cross-linking and resulted in the formation of dimers and trimers. polyacrylamide 23-37 ovalbumin (SERPINB14) Gallus gallus 95-104 30101285-4 2019 Sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis showed that the exposure of ovalbumin to hydroxyl radicals caused self-cross-linking and resulted in the formation of dimers and trimers. Hydroxyl Radical 108-125 ovalbumin (SERPINB14) Gallus gallus 95-104 30101285-7 2019 Hydroxyl radical-induced oxidation changed the surface chemical groups and structures of ovalbumin, thereby affecting the surface properties. Hydroxyl Radical 0-16 ovalbumin (SERPINB14) Gallus gallus 89-98 29580801-8 2018 Moreover, Con A-Cu(II)-IDA-MCM displays weak nonspecific adsorption for the impurities and is able to successfully enrich glycoprotein ovalbumin (OVA) from diluted chicken egg white. con a-cu(ii)-ida-mcm 10-30 ovalbumin (SERPINB14) Gallus gallus 135-144 29580801-8 2018 Moreover, Con A-Cu(II)-IDA-MCM displays weak nonspecific adsorption for the impurities and is able to successfully enrich glycoprotein ovalbumin (OVA) from diluted chicken egg white. con a-cu(ii)-ida-mcm 10-30 ovalbumin (SERPINB14) Gallus gallus 146-149 29149729-6 2018 The generated Au@cew NPs are mainly encapsulated by ovalbumin, in which the surface is decorated by abundant hybrid mannose and Galbeta(1 4)GlcNAc-terminated glycan ligands. Mannose 116-123 ovalbumin (SERPINB14) Gallus gallus 52-61 29149729-6 2018 The generated Au@cew NPs are mainly encapsulated by ovalbumin, in which the surface is decorated by abundant hybrid mannose and Galbeta(1 4)GlcNAc-terminated glycan ligands. galbeta 128-135 ovalbumin (SERPINB14) Gallus gallus 52-61 29149729-6 2018 The generated Au@cew NPs are mainly encapsulated by ovalbumin, in which the surface is decorated by abundant hybrid mannose and Galbeta(1 4)GlcNAc-terminated glycan ligands. 2-acetamido-2-deoxy-4-O-(beta-2-acetamid-2-deoxyglucopyranosyl)glucopyranose 140-146 ovalbumin (SERPINB14) Gallus gallus 52-61 29149729-6 2018 The generated Au@cew NPs are mainly encapsulated by ovalbumin, in which the surface is decorated by abundant hybrid mannose and Galbeta(1 4)GlcNAc-terminated glycan ligands. Polysaccharides 158-164 ovalbumin (SERPINB14) Gallus gallus 52-61 26842584-5 2016 METHODS: Released N-glycans from the glycoproteins bovine fetuin, ribonuclease B, chicken ovalbumin, and porcine thyroglobulin were reduced with sodium cyanoborohydride and both negative ion CID spectra and ion mobility properties of their phosphate adducts were examined with a Waters Synapt G2Si travelling-wave ion mobility mass spectrometer with electrospray sample introduction. n-glycans 18-27 ovalbumin (SERPINB14) Gallus gallus 90-99 27534448-2 2016 In rodent models, acute respiratory, subcutaneous, and direct immune cell exposure to CBNPs has been shown to enhance allergic sensitization to co-administered ovalbumin (OVA) protein from chicken egg. cbnps 86-91 ovalbumin (SERPINB14) Gallus gallus 160-169 27534448-2 2016 In rodent models, acute respiratory, subcutaneous, and direct immune cell exposure to CBNPs has been shown to enhance allergic sensitization to co-administered ovalbumin (OVA) protein from chicken egg. cbnps 86-91 ovalbumin (SERPINB14) Gallus gallus 171-174 28220659-0 2017 In situ self-assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high-efficiency separation of ovalbumin. graphene oxide 31-45 ovalbumin (SERPINB14) Gallus gallus 133-142 28220659-0 2017 In situ self-assembled reduced graphene oxide aerogel embedded with nickel oxide nanoparticles for the high-efficiency separation of ovalbumin. nickel monoxide 68-80 ovalbumin (SERPINB14) Gallus gallus 133-142 28220659-7 2017 The adsorption behavior of ovalbumin on the reduced graphene oxide composite well fitted to the Langmuir adsorption model, and a corresponding theoretical maximum adsorption capacity was 1695.2 mg/g. graphene oxide 52-66 ovalbumin (SERPINB14) Gallus gallus 27-36 28220659-8 2017 A sodium dodecyl sulfate polyacrylamide gel electrophoresis assay demonstrated that the aerogel could selectively isolate ovalbumin from chicken egg white. Sodium Dodecyl Sulfate 2-24 ovalbumin (SERPINB14) Gallus gallus 122-131 28220659-8 2017 A sodium dodecyl sulfate polyacrylamide gel electrophoresis assay demonstrated that the aerogel could selectively isolate ovalbumin from chicken egg white. polyacrylamide 25-39 ovalbumin (SERPINB14) Gallus gallus 122-131 27477117-3 2016 Some isomers, such as Man3 GlcNAc3 from chicken ovalbumin and Man3 GlcNAc3 Fuc1 from thyroglobulin could be partially resolved and identified by their negative ion fragmentation spectra obtained by collision-induced decomposition (CID). man3 glcnac3 22-34 ovalbumin (SERPINB14) Gallus gallus 48-57 26842584-5 2016 METHODS: Released N-glycans from the glycoproteins bovine fetuin, ribonuclease B, chicken ovalbumin, and porcine thyroglobulin were reduced with sodium cyanoborohydride and both negative ion CID spectra and ion mobility properties of their phosphate adducts were examined with a Waters Synapt G2Si travelling-wave ion mobility mass spectrometer with electrospray sample introduction. sodium cyanoborohydride 145-168 ovalbumin (SERPINB14) Gallus gallus 90-99 26842584-5 2016 METHODS: Released N-glycans from the glycoproteins bovine fetuin, ribonuclease B, chicken ovalbumin, and porcine thyroglobulin were reduced with sodium cyanoborohydride and both negative ion CID spectra and ion mobility properties of their phosphate adducts were examined with a Waters Synapt G2Si travelling-wave ion mobility mass spectrometer with electrospray sample introduction. Phosphates 240-249 ovalbumin (SERPINB14) Gallus gallus 90-99 26886334-3 2016 Poly(lactic-co-glycolic acid) particles (size range: 250-600 nm) were successfully formulated to include PET lipid A and/or the model tumor antigen, chicken ovalbumin (OVA). Polylactic Acid-Polyglycolic Acid Copolymer 0-29 ovalbumin (SERPINB14) Gallus gallus 157-166 25982752-0 2015 Transition of serine residues to the D-form during the conversion of ovalbumin into heat stable S-ovalbumin. Serine 14-20 ovalbumin (SERPINB14) Gallus gallus 69-78 25982752-0 2015 Transition of serine residues to the D-form during the conversion of ovalbumin into heat stable S-ovalbumin. Serine 14-20 ovalbumin (SERPINB14) Gallus gallus 98-107 25982752-5 2015 A time-dependent increase in the D-Ser contents in native ovalbumin was observed over a period of 7 days, reaching approximately 8%. D-serine 33-38 ovalbumin (SERPINB14) Gallus gallus 58-67 25982752-2 2015 Our previous X-ray crystallographic study indicated that S-ovalbumin contains three D-Ser residues (S164, S236, and S320), which may account for its thermostability. D-serine 84-89 ovalbumin (SERPINB14) Gallus gallus 59-68 25982752-3 2015 Here, we confirmed the presence of these D-Ser residues in ovalbumin using a technique combining deuterium labeling of alpha-protons of amino acids and liquid chromatography-tandem mass spectrometry (LC-MS/MS). D-serine 41-46 ovalbumin (SERPINB14) Gallus gallus 59-68 25982752-3 2015 Here, we confirmed the presence of these D-Ser residues in ovalbumin using a technique combining deuterium labeling of alpha-protons of amino acids and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Deuterium 97-106 ovalbumin (SERPINB14) Gallus gallus 59-68 25982752-4 2015 Ovalbumin from chicken egg white and recombinant ovalbumin were incubated for approximately 12 days at pH 9.5 and 37 C. They were then hydrolyzed in DCl/D2O vapor, derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and analyzed by LC-MS/MS. dcl 149-152 ovalbumin (SERPINB14) Gallus gallus 0-9 25982752-4 2015 Ovalbumin from chicken egg white and recombinant ovalbumin were incubated for approximately 12 days at pH 9.5 and 37 C. They were then hydrolyzed in DCl/D2O vapor, derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and analyzed by LC-MS/MS. dcl 149-152 ovalbumin (SERPINB14) Gallus gallus 49-58 25982752-4 2015 Ovalbumin from chicken egg white and recombinant ovalbumin were incubated for approximately 12 days at pH 9.5 and 37 C. They were then hydrolyzed in DCl/D2O vapor, derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and analyzed by LC-MS/MS. Deuterium Oxide 153-156 ovalbumin (SERPINB14) Gallus gallus 0-9 25982752-4 2015 Ovalbumin from chicken egg white and recombinant ovalbumin were incubated for approximately 12 days at pH 9.5 and 37 C. They were then hydrolyzed in DCl/D2O vapor, derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and analyzed by LC-MS/MS. Deuterium Oxide 153-156 ovalbumin (SERPINB14) Gallus gallus 49-58 25982752-4 2015 Ovalbumin from chicken egg white and recombinant ovalbumin were incubated for approximately 12 days at pH 9.5 and 37 C. They were then hydrolyzed in DCl/D2O vapor, derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and analyzed by LC-MS/MS. 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole 181-218 ovalbumin (SERPINB14) Gallus gallus 0-9 25982752-4 2015 Ovalbumin from chicken egg white and recombinant ovalbumin were incubated for approximately 12 days at pH 9.5 and 37 C. They were then hydrolyzed in DCl/D2O vapor, derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), and analyzed by LC-MS/MS. 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole 181-218 ovalbumin (SERPINB14) Gallus gallus 49-58 26335373-6 2015 Application of the strategy to chicken ovalbumin, normal mouse mammary epithelial cells (NMuMG), and human serum resulted in detection of 5, 6, and 11 additional N-glycan structures, respectively. n-glycan 162-170 ovalbumin (SERPINB14) Gallus gallus 39-48 32262779-0 2015 Preparation of a cobalt mono-substituted silicotungstic acid doped with aniline for the selective adsorption of ovalbumin. cobalt mono-substituted silicotungstic acid 17-60 ovalbumin (SERPINB14) Gallus gallus 112-121 32262779-0 2015 Preparation of a cobalt mono-substituted silicotungstic acid doped with aniline for the selective adsorption of ovalbumin. aniline 72-79 ovalbumin (SERPINB14) Gallus gallus 112-121 32262779-3 2015 5.0 mg of SiW11Co-PANI composite gives rise to an adsorption efficiency of >70% for 100 mg L-1 ovalbumin in 1.0 mL of sample solution within a wide pH range of 3-9, and a maximum adsorption efficiency of 92% is achieved at pH 9. siw11co-pani 10-22 ovalbumin (SERPINB14) Gallus gallus 95-104 32262779-5 2015 The retained ovalbumin could be readily recovered by using a 0.1 mol L-1 phosphate buffer at pH 5.6 as a stripping reagent, providing a recovery of 84.4%. Phosphates 73-82 ovalbumin (SERPINB14) Gallus gallus 13-22 32262779-7 2015 The SiW11Co-PANI composite has been applied for the selective adsorption of ovalbumin from the chicken egg white, and SDS-PAGE assay demonstrates that high purity of ovalbumin is obtained. Sodium Dodecyl Sulfate 118-121 ovalbumin (SERPINB14) Gallus gallus 76-85 32262779-7 2015 The SiW11Co-PANI composite has been applied for the selective adsorption of ovalbumin from the chicken egg white, and SDS-PAGE assay demonstrates that high purity of ovalbumin is obtained. Sodium Dodecyl Sulfate 118-121 ovalbumin (SERPINB14) Gallus gallus 166-175 23181906-5 2012 Furthermore, they were covalently linked to Sepharose and used as an affinity matrix for ovalbumin depletion. Sepharose 44-53 ovalbumin (SERPINB14) Gallus gallus 89-98 25683505-0 2015 Effect of nitric oxide on conformational changes of ovalbumin accompanying self-assembly into non-disease-associated fibrils. Nitric Oxide 10-22 ovalbumin (SERPINB14) Gallus gallus 52-61 24446294-6 2014 Moreover, the relative OVA expression level in pigeon oviduct epithelial cells could be upregulated by a constant concentration of steroid hormones. Steroids 131-147 ovalbumin (SERPINB14) Gallus gallus 23-26 24010128-8 2013 The proposed derivatization technique is successfully applied to the profiling of N-linked glycans derived from chicken ovalbumin. n-linked glycans 82-98 ovalbumin (SERPINB14) Gallus gallus 120-129 23995439-5 2013 At pH 6.0, use of 5 mg PIL@SiO2 nanocomposite results in a sorption efficiency of up to 95 % for 200 mg L(-1) ovalbumin in 1 mL sample solution. Silicon Dioxide 27-31 ovalbumin (SERPINB14) Gallus gallus 110-119 23995439-8 2013 The retained ovalbumin is recovered by elution with 0.2 % SDS solution. Sodium Dodecyl Sulfate 58-61 ovalbumin (SERPINB14) Gallus gallus 13-22 23995439-10 2013 In summary, high-purity ovalbumin is isolated from chicken egg-white by use of the PIL@SiO2 nanocomposite as adsorbent. Silicon Dioxide 87-91 ovalbumin (SERPINB14) Gallus gallus 24-33 23090025-2 2013 Conformational changes on ovalbumin at various concentrations of glyoxal, ethylene glycol (EG) and polyethylene glycol-400 (PEG-400) were investigated by fluorescence spectroscopy, circular dichroism, attenuated total reflection Fourier transform infra red spectroscopy, 8-anilino-1-naphthalenesulfonic acid and thioflavin T assay. Glyoxal 65-72 ovalbumin (SERPINB14) Gallus gallus 26-35 23090025-2 2013 Conformational changes on ovalbumin at various concentrations of glyoxal, ethylene glycol (EG) and polyethylene glycol-400 (PEG-400) were investigated by fluorescence spectroscopy, circular dichroism, attenuated total reflection Fourier transform infra red spectroscopy, 8-anilino-1-naphthalenesulfonic acid and thioflavin T assay. Ethylene Glycol 74-89 ovalbumin (SERPINB14) Gallus gallus 26-35 23090025-2 2013 Conformational changes on ovalbumin at various concentrations of glyoxal, ethylene glycol (EG) and polyethylene glycol-400 (PEG-400) were investigated by fluorescence spectroscopy, circular dichroism, attenuated total reflection Fourier transform infra red spectroscopy, 8-anilino-1-naphthalenesulfonic acid and thioflavin T assay. Ethylene Glycol 91-93 ovalbumin (SERPINB14) Gallus gallus 26-35 23090025-2 2013 Conformational changes on ovalbumin at various concentrations of glyoxal, ethylene glycol (EG) and polyethylene glycol-400 (PEG-400) were investigated by fluorescence spectroscopy, circular dichroism, attenuated total reflection Fourier transform infra red spectroscopy, 8-anilino-1-naphthalenesulfonic acid and thioflavin T assay. polyethylene glycol 400 99-122 ovalbumin (SERPINB14) Gallus gallus 26-35 23090025-2 2013 Conformational changes on ovalbumin at various concentrations of glyoxal, ethylene glycol (EG) and polyethylene glycol-400 (PEG-400) were investigated by fluorescence spectroscopy, circular dichroism, attenuated total reflection Fourier transform infra red spectroscopy, 8-anilino-1-naphthalenesulfonic acid and thioflavin T assay. polyethylene glycol 400 124-131 ovalbumin (SERPINB14) Gallus gallus 26-35 23090025-2 2013 Conformational changes on ovalbumin at various concentrations of glyoxal, ethylene glycol (EG) and polyethylene glycol-400 (PEG-400) were investigated by fluorescence spectroscopy, circular dichroism, attenuated total reflection Fourier transform infra red spectroscopy, 8-anilino-1-naphthalenesulfonic acid and thioflavin T assay. 8-anilino-1-naphthalenesulfonic acid 271-307 ovalbumin (SERPINB14) Gallus gallus 26-35 23090025-2 2013 Conformational changes on ovalbumin at various concentrations of glyoxal, ethylene glycol (EG) and polyethylene glycol-400 (PEG-400) were investigated by fluorescence spectroscopy, circular dichroism, attenuated total reflection Fourier transform infra red spectroscopy, 8-anilino-1-naphthalenesulfonic acid and thioflavin T assay. thioflavin T 312-324 ovalbumin (SERPINB14) Gallus gallus 26-35 23090025-3 2013 A partially folded state of ovalbumin at 50 % v/v glyoxal was detected that preceded the onset of the aggregation process at the maximum concentration (90 % v/v) of this aldehyde. Glyoxal 50-57 ovalbumin (SERPINB14) Gallus gallus 28-37 23090025-3 2013 A partially folded state of ovalbumin at 50 % v/v glyoxal was detected that preceded the onset of the aggregation process at the maximum concentration (90 % v/v) of this aldehyde. Aldehydes 170-178 ovalbumin (SERPINB14) Gallus gallus 28-37 23090025-4 2013 Aggregates of ovalbumin in the presence EG and PEG-400 were deduced at 70 and 80 % v/v respectively. Ethylene Glycol 40-42 ovalbumin (SERPINB14) Gallus gallus 14-23 23090025-4 2013 Aggregates of ovalbumin in the presence EG and PEG-400 were deduced at 70 and 80 % v/v respectively. polyethylene glycol 400 47-54 ovalbumin (SERPINB14) Gallus gallus 14-23 23090025-5 2013 Maximum aggregation of ovalbumin was observed at 80 % v/v PEG-400, followed by 70 % v/v EG and 90 % v/v glyoxal. polyethylene glycol 400 58-65 ovalbumin (SERPINB14) Gallus gallus 23-32 23090025-5 2013 Maximum aggregation of ovalbumin was observed at 80 % v/v PEG-400, followed by 70 % v/v EG and 90 % v/v glyoxal. Ethylene Glycol 59-61 ovalbumin (SERPINB14) Gallus gallus 23-32 23090025-5 2013 Maximum aggregation of ovalbumin was observed at 80 % v/v PEG-400, followed by 70 % v/v EG and 90 % v/v glyoxal. Glyoxal 104-111 ovalbumin (SERPINB14) Gallus gallus 23-32 23505104-2 2013 METHODS: The derivative of DES (DES-HS) was synthesized from diethylstilbestrol, ethyl bromoacetate,bovine serum albumin (BSA) and chicken ovalbumin (OVA) with the nucleophilic substitution reaction; the compound was identified by electrospray ionization mass spectrometry(ESI-MS). Diethylstilbestrol 27-30 ovalbumin (SERPINB14) Gallus gallus 139-148 23505104-2 2013 METHODS: The derivative of DES (DES-HS) was synthesized from diethylstilbestrol, ethyl bromoacetate,bovine serum albumin (BSA) and chicken ovalbumin (OVA) with the nucleophilic substitution reaction; the compound was identified by electrospray ionization mass spectrometry(ESI-MS). des-hs 32-38 ovalbumin (SERPINB14) Gallus gallus 139-148 20194691-0 2010 3-hydroxyphthalic anhydride-modified chicken ovalbumin exhibits potent and broad anti-HIV-1 activity: a potential microbicide for preventing sexual transmission of HIV-1. 3-hydroxyphthalic anhydride 0-27 ovalbumin (SERPINB14) Gallus gallus 45-54 22991893-11 2012 Five of the major known proteins including three glycoisoforms of ovalbumin in chicken egg white were identified in a single run on the GO@column with phosphate buffer (5 mM, pH 7.0) and an applied voltage of 20 kV. Phosphates 151-160 ovalbumin (SERPINB14) Gallus gallus 66-75 23281756-7 2012 In the allantoic fluid, especially on day 7, considerable proportions of cystatin and ovalbumin were phosphorylated and contained phosphorylated serine. Serine 145-151 ovalbumin (SERPINB14) Gallus gallus 86-95 21239999-2 2011 Our previous studies demonstrated that 3-hydroxyphthalic anhydride-modified chicken ovalbumin (HP-OVA) exhibited potent antiviral activity against a broad spectrum of HIV, simian immunodeficiency virus, and herpes simplex virus, making it a promising candidate as a component of combination microbicide. 3-hydroxyphthalic anhydride 39-66 ovalbumin (SERPINB14) Gallus gallus 84-93 21715713-5 2011 Results showed that oviduct-specific levels of avidin, ovalbumin, ovomucin, lysozyme, ESR1, and PGR gene expression were significantly elevated in steroid hormone-treated OECs compared with those of untreated cells (P < 0.05). Steroids 147-162 ovalbumin (SERPINB14) Gallus gallus 55-64 21728232-4 2011 Polyacrylamide gels containing immobilized Fe(III) ions were used to study the electrophoretic behavior of two model proteins differing in their phosphate group content: chicken ovalbumin and bovine alpha-casein. polyacrylamide 0-14 ovalbumin (SERPINB14) Gallus gallus 178-187 21159849-4 2011 Afterwards, mice were sensitized to chicken egg ovalbumin (OVA) precipitated in aluminium sulphate and then intranasally challenged with OVA to induce allergic lung inflammation. aluminum sulfate 80-98 ovalbumin (SERPINB14) Gallus gallus 48-57 21159849-4 2011 Afterwards, mice were sensitized to chicken egg ovalbumin (OVA) precipitated in aluminium sulphate and then intranasally challenged with OVA to induce allergic lung inflammation. aluminum sulfate 80-98 ovalbumin (SERPINB14) Gallus gallus 59-62 20147736-3 2010 To drive tissue-specific expression of rhIL1RN, a 1.35-kb fragment of the chicken ovalbumin promoter, which contains both the steroid-dependent regulatory element and the negative regulatory element, was used. Steroids 126-133 ovalbumin (SERPINB14) Gallus gallus 82-91 20194691-3 2010 Here we report that 3-hydroxyphthalic anhydride-modified chicken ovalbumin (HP-OVA) exhibits potent antiviral activity against a broad spectrum of human immunodeficiency virus type 1 (HIV-1) isolates with different genotypes and biotypes. 3-hydroxyphthalic anhydride 20-47 ovalbumin (SERPINB14) Gallus gallus 65-74 15838100-3 2004 The key discovery that set the scientific tone for the lab over the ensuing few decades began with the discovery that steroid hormones induced the nuclear production of specific mRNAs for ovalbumin and avidin in the chick oviduct. Steroids 118-134 ovalbumin (SERPINB14) Gallus gallus 188-197 19558425-4 2009 Sensitized rats received chicken egg albumin (EA, 1 mg mL(-1)) in drinking water for 2 weeks (day 1-14). Drinking Water 66-80 ovalbumin (SERPINB14) Gallus gallus 33-44 19341784-1 2009 Although the ovalbumin (Ov) gene has served as a model to study tissue-specific, steroid hormone-induced gene expression in vertebrates for decades, the mechanisms responsible for regulating this gene remain elusive. Steroids 81-96 ovalbumin (SERPINB14) Gallus gallus 13-22 19341784-1 2009 Although the ovalbumin (Ov) gene has served as a model to study tissue-specific, steroid hormone-induced gene expression in vertebrates for decades, the mechanisms responsible for regulating this gene remain elusive. Steroids 81-96 ovalbumin (SERPINB14) Gallus gallus 24-26 18672356-4 2008 The equilibrium constants of the newly selected tetrapeptides increased slightly respect to the dipeptides (Pro-Lys-His-Phe for Npt II, K(eq) 7.88 x 10(4)M(-1); Trp-Gln-Ala-Phe for Cry 1A, K(eq) 5.65 x 10(4)M(-1)), but selectivity towards other proteins (wheat gliadins, bovine gamma-globulins, bovine serum albumin and chicken ovalbumin) became higher. Dipeptides 96-106 ovalbumin (SERPINB14) Gallus gallus 328-337 18672356-4 2008 The equilibrium constants of the newly selected tetrapeptides increased slightly respect to the dipeptides (Pro-Lys-His-Phe for Npt II, K(eq) 7.88 x 10(4)M(-1); Trp-Gln-Ala-Phe for Cry 1A, K(eq) 5.65 x 10(4)M(-1)), but selectivity towards other proteins (wheat gliadins, bovine gamma-globulins, bovine serum albumin and chicken ovalbumin) became higher. Pro-Lys-His 108-119 ovalbumin (SERPINB14) Gallus gallus 328-337 18672356-4 2008 The equilibrium constants of the newly selected tetrapeptides increased slightly respect to the dipeptides (Pro-Lys-His-Phe for Npt II, K(eq) 7.88 x 10(4)M(-1); Trp-Gln-Ala-Phe for Cry 1A, K(eq) 5.65 x 10(4)M(-1)), but selectivity towards other proteins (wheat gliadins, bovine gamma-globulins, bovine serum albumin and chicken ovalbumin) became higher. Phenylalanine 120-123 ovalbumin (SERPINB14) Gallus gallus 328-337 18179854-2 2008 It has been reported previously that certain commonly used phthalate plasticizers, such as di-(2-ethylhexyl) phthalate (DEHP), are able to modify immune responses induced in mice by the common hens" egg allergen ovalbumin (OVA). phthalic acid 59-68 ovalbumin (SERPINB14) Gallus gallus 212-221 18179854-2 2008 It has been reported previously that certain commonly used phthalate plasticizers, such as di-(2-ethylhexyl) phthalate (DEHP), are able to modify immune responses induced in mice by the common hens" egg allergen ovalbumin (OVA). Diethylhexyl Phthalate 91-118 ovalbumin (SERPINB14) Gallus gallus 212-221 18179854-2 2008 It has been reported previously that certain commonly used phthalate plasticizers, such as di-(2-ethylhexyl) phthalate (DEHP), are able to modify immune responses induced in mice by the common hens" egg allergen ovalbumin (OVA). Diethylhexyl Phthalate 120-124 ovalbumin (SERPINB14) Gallus gallus 212-221 16380189-2 2006 TMC nanoparticles were prepared by ionic crosslinking of TMC solution (with or without ovalbumin) with tripolyphosphate, at ambient temperature while stirring. N-trimethyl chitosan chloride 0-3 ovalbumin (SERPINB14) Gallus gallus 87-96 15751107-1 2005 N-Linked glycans from bovine ribonuclease B, chicken ovalbumin, bovine fetuin, porcine thyroglobulin and human alpha(1)-acid glycoprotein were derivatized with 2-aminobenzoic acid by reductive amination and their tandem mass spectra were recorded by negative ion electrospray ionization with a quadrupole time-of-flight mass spectrometer. n-linked glycans 0-16 ovalbumin (SERPINB14) Gallus gallus 53-62 15659378-0 2005 Reversible self-association of ovalbumin at air-water interfaces and the consequences for the exerted surface pressure. Water 48-53 ovalbumin (SERPINB14) Gallus gallus 31-40 14719827-2 2003 A gene encoding for chicken ovalbumin (Gad dI) was isolated from chicken oviduct by PCR amplification and was cloned under the control of T5 promoter fused with a six-histidine tag at the N-terminal end. Histidine 167-176 ovalbumin (SERPINB14) Gallus gallus 28-37 14983084-2 2003 Native ovalbumin (N-OVA) is converted on the hours time-scale into more heat-stable forms denoted I- (intermediate) and S-OVA, that have denaturation temperatures 4.8 and 8.4 degrees C, respectively, higher than that of N-OVA. n-ova 18-23 ovalbumin (SERPINB14) Gallus gallus 7-16 14983084-2 2003 Native ovalbumin (N-OVA) is converted on the hours time-scale into more heat-stable forms denoted I- (intermediate) and S-OVA, that have denaturation temperatures 4.8 and 8.4 degrees C, respectively, higher than that of N-OVA. n-ova 220-225 ovalbumin (SERPINB14) Gallus gallus 7-16 15283599-5 2004 Despite the general recognition that the production of signals of oligosaccharides under MALDI conditions would be highly dependent on the matrix, most of the known N-glycans from chicken ovalbumin could be detected upon Cye derivatization nearly independent of the kind of matrix tested (e.g., nor-harman, 2,5-dihydroxybenzoic acid and alpha-cyano-4-hydroxycinnamic acid) without spoiling the signal strength. n-glycans 165-174 ovalbumin (SERPINB14) Gallus gallus 188-197 15283599-5 2004 Despite the general recognition that the production of signals of oligosaccharides under MALDI conditions would be highly dependent on the matrix, most of the known N-glycans from chicken ovalbumin could be detected upon Cye derivatization nearly independent of the kind of matrix tested (e.g., nor-harman, 2,5-dihydroxybenzoic acid and alpha-cyano-4-hydroxycinnamic acid) without spoiling the signal strength. 45-(3-AMINOPROPYL)-5,11,22,28,34-PENTAMETHYL-3,9,15,20,26,32,38,43-OCTAOXO-2,5,8,14,19,22,25,28,31,34,37,42,45,48-TETRADECAAZA-11-AZONIAHEPTACYCLO[42.2.1.1~4,7~.1~10,13~.1~21,24~.1~27,30~.1~33,36~]DOPENTACONTA-1(46),4(52),6,10(51),12,21(50),23,27(49),29,33(48),35,44(47)-DODECAENE 221-224 ovalbumin (SERPINB14) Gallus gallus 188-197 12609095-5 2002 After multiple ovalbumin exposures, airway responsiveness to intravenous injection of acetylcholine decreased significantly (-LogPC(200): 4.006 +/- 0.554 vs 2.059 +/- 0.262; P < 0.01). Acetylcholine 86-99 ovalbumin (SERPINB14) Gallus gallus 15-24 14600204-2 2003 In this study, the immunodominant IgE-binding epitopes of ovalbumin were mapped using arrays of overlapping peptides synthesized on activated cellulose membranes. Cellulose 142-151 ovalbumin (SERPINB14) Gallus gallus 58-67 9403346-1 1997 The effect of the histamine H2-receptor antagonist, cimetidine, on the cutaneous Arthus-like hypersensitivity to oxazolone elicited injecting subcutaneously oxazolone conjugated to egg-albumin (EA-OX) has been examined in the chicken. Cimetidine 52-62 ovalbumin (SERPINB14) Gallus gallus 181-192 10833030-0 2000 Composition of N-linked carbohydrates from ovalbumin and co-purified glycoproteins. n-linked carbohydrates 15-37 ovalbumin (SERPINB14) Gallus gallus 43-52 10833030-4 2000 Ovalbumin was found to be glycosylated mainly with high-mannose and hybrid structures, consistent with profiles obtained on the intact glycoprotein by electrospray. Mannose 56-63 ovalbumin (SERPINB14) Gallus gallus 0-9 10833030-5 2000 The other glycoproteins contained mainly larger, complex glycans with up to five antennae, many of which had earlier been associated with ovalbumin. Polysaccharides 57-64 ovalbumin (SERPINB14) Gallus gallus 138-147 10457454-6 1999 Their glycoform patterns were highly similar except for the conspicuous decrease in quantity of four glycoforms in the ovalbumin containing less mannose, compared to that of the other with more mannose. Mannose 145-152 ovalbumin (SERPINB14) Gallus gallus 119-128 12207461-1 2002 Bisphenol A was coupled, after derivatization into a suitable hapten, to bovine serum albumin and ovalbumin in order to produce immunizing and coating antigens. bisphenol A 0-11 ovalbumin (SERPINB14) Gallus gallus 98-107 10073574-10 1999 These results demonstrate that the Ov NRE contains not only sites responsible for the repression of the gene but also a positive element that is required for responsiveness to steroid hormones. Steroids 176-192 ovalbumin (SERPINB14) Gallus gallus 35-37 9832435-3 1998 The ovalbumin gene contains one such unit, known as the steroid-dependent regulatory element. Steroids 56-63 ovalbumin (SERPINB14) Gallus gallus 4-13 9541491-3 1998 A single exposure of the EC sensitized mice to aerosolized OVA induced eosinophilia in the bronchoalveolar lavage fluid and airway hyperresponsiveness to intravenous methacholine as assessed by measurement of pulmonary dynamic compliance (Cdyn). Methacholine Chloride 166-178 ovalbumin (SERPINB14) Gallus gallus 59-62 9541491-3 1998 A single exposure of the EC sensitized mice to aerosolized OVA induced eosinophilia in the bronchoalveolar lavage fluid and airway hyperresponsiveness to intravenous methacholine as assessed by measurement of pulmonary dynamic compliance (Cdyn). cdyn 239-243 ovalbumin (SERPINB14) Gallus gallus 59-62 9403346-1 1997 The effect of the histamine H2-receptor antagonist, cimetidine, on the cutaneous Arthus-like hypersensitivity to oxazolone elicited injecting subcutaneously oxazolone conjugated to egg-albumin (EA-OX) has been examined in the chicken. Oxazolone 113-122 ovalbumin (SERPINB14) Gallus gallus 181-192 8947831-4 1996 The half-life of OVA mRNA as determined using a transcription inhibitor (actinomycin D) was estimated to be about 24 h irrespective of the hormone treatment, though the half-life was about 6 h in the absence of hormones. Dactinomycin 73-86 ovalbumin (SERPINB14) Gallus gallus 17-20 9175273-5 1997 By using free-solution CE with polyacrylamide-coated capillaries and boric acid-alcohol amine (BA) buffers, ovalbumin of agarose electrophoresis purity can be split into more than twenty peaks at pH 8.0 +/- 0.3 (0.2-0.4 mol/l BA). polyacrylamide 31-45 ovalbumin (SERPINB14) Gallus gallus 108-117 9175273-5 1997 By using free-solution CE with polyacrylamide-coated capillaries and boric acid-alcohol amine (BA) buffers, ovalbumin of agarose electrophoresis purity can be split into more than twenty peaks at pH 8.0 +/- 0.3 (0.2-0.4 mol/l BA). boric acid-alcohol amine 69-93 ovalbumin (SERPINB14) Gallus gallus 108-117 9175273-5 1997 By using free-solution CE with polyacrylamide-coated capillaries and boric acid-alcohol amine (BA) buffers, ovalbumin of agarose electrophoresis purity can be split into more than twenty peaks at pH 8.0 +/- 0.3 (0.2-0.4 mol/l BA). Barium 95-97 ovalbumin (SERPINB14) Gallus gallus 108-117 9175273-5 1997 By using free-solution CE with polyacrylamide-coated capillaries and boric acid-alcohol amine (BA) buffers, ovalbumin of agarose electrophoresis purity can be split into more than twenty peaks at pH 8.0 +/- 0.3 (0.2-0.4 mol/l BA). Sepharose 121-128 ovalbumin (SERPINB14) Gallus gallus 108-117 9175273-5 1997 By using free-solution CE with polyacrylamide-coated capillaries and boric acid-alcohol amine (BA) buffers, ovalbumin of agarose electrophoresis purity can be split into more than twenty peaks at pH 8.0 +/- 0.3 (0.2-0.4 mol/l BA). Barium 226-228 ovalbumin (SERPINB14) Gallus gallus 108-117 9149392-1 1997 The role of estrogen receptor on ovalbumin mRNA induction by steroid hormones was investigated in primary cultures of oviduct cells from estrogen-stimulated immature chicks of genetically selected high- and low-albumen egg laying lines (H- and L-lines). Steroids 61-77 ovalbumin (SERPINB14) Gallus gallus 33-42 8947831-0 1996 Steroid hormones differentially induce transcription of the chicken ovalbumin gene, but stabilize the mRNA with the same half-life. Steroids 0-16 ovalbumin (SERPINB14) Gallus gallus 68-77 8947831-1 1996 The stabilization of chicken ovalbumin (OVA) mRNA by different classes of steroid hormones (estrogen, progesterone, glucocorticoid, and androgen) was studied in the oviducts of chicks treated with combinations of four steroids. Steroids 74-90 ovalbumin (SERPINB14) Gallus gallus 40-43 8947831-5 1996 These results suggested that the prolongation of the half-life of OVA mRNA by steroid hormones is constant irrespective of differential transcription rates of the OVA gene. Steroids 78-94 ovalbumin (SERPINB14) Gallus gallus 66-69 8916422-5 1996 The method is illustrated by the analysis of isolated glycans and complex mixtures derived from chicken ovalbumin and human immunoglobulin G. Polysaccharides 54-61 ovalbumin (SERPINB14) Gallus gallus 104-113 8546694-3 1996 Run-on analysis with oviduct nuclei isolated from E2-treated chicks showed that TAM treatment completely blocked E2-induced transcription of the OVA gene within 24 h without affecting ER gene expression. Tamoxifen 80-83 ovalbumin (SERPINB14) Gallus gallus 145-148 8819508-9 1996 However, GaAs-exposed macrophages activated hen egg lysozyme- and chicken ovalbumin-specific T cells as efficiently as vehicle control cells. gallium arsenide 9-13 ovalbumin (SERPINB14) Gallus gallus 74-83 8901111-0 1996 Steroid hormone-induced expression of the chicken ovalbumin gene and the levels of nuclear steroid hormone receptors in chick oviduct. Steroids 0-15 ovalbumin (SERPINB14) Gallus gallus 50-59 8901111-1 1996 The induction of the chicken ovalbumin (OVA) gene by different classes of steroid hormones and the mRNA levels of estrogen (ER), progesterone (PR), and glucocorticoid (GR) receptors were studied in chick oviducts. Steroids 74-90 ovalbumin (SERPINB14) Gallus gallus 29-38 8901111-1 1996 The induction of the chicken ovalbumin (OVA) gene by different classes of steroid hormones and the mRNA levels of estrogen (ER), progesterone (PR), and glucocorticoid (GR) receptors were studied in chick oviducts. Steroids 74-90 ovalbumin (SERPINB14) Gallus gallus 40-43 8833539-17 1996 There was significantly more reducible selenium in ovalbumin from control eggs than from all other samples but even so non-reducible selenium accounted for two thirds of the selenium present. Selenium 39-47 ovalbumin (SERPINB14) Gallus gallus 51-60 8628267-0 1996 Regulation of the chicken ovalbumin gene by estrogen and corticosterone requires a novel DNA element that binds a labile protein, Chirp-1. Corticosterone 57-71 ovalbumin (SERPINB14) Gallus gallus 26-35 8628267-1 1996 Because induction of the chicken ovalbumin (Ov) gene by steroid hormones requires concomitant protein synthesis, efforts have focused on defining the binding site in the Ov gene for a labile transcription factor. Steroids 56-72 ovalbumin (SERPINB14) Gallus gallus 33-42 8628267-1 1996 Because induction of the chicken ovalbumin (Ov) gene by steroid hormones requires concomitant protein synthesis, efforts have focused on defining the binding site in the Ov gene for a labile transcription factor. Steroids 56-72 ovalbumin (SERPINB14) Gallus gallus 44-46 8628267-3 1996 To ascertain whether estrogen and glucocorticoid affect the binding of this labile protein, genomic footprinting of the Ov gene was done by treating primary oviduct cell cultures with dimethyl sulfate. dimethyl sulfate 184-200 ovalbumin (SERPINB14) Gallus gallus 120-122 8628267-11 1996 These data support the contention that the ovalbumin gene is regulated by a steroid hormone-induced transcriptional cascade that culminates in the binding of chicken ovalbumin induced regulatory protein or protein complex (Chirp-I) to a DNA element from -891 to -878 in the SDRE. Steroids 76-91 ovalbumin (SERPINB14) Gallus gallus 43-52 8628267-11 1996 These data support the contention that the ovalbumin gene is regulated by a steroid hormone-induced transcriptional cascade that culminates in the binding of chicken ovalbumin induced regulatory protein or protein complex (Chirp-I) to a DNA element from -891 to -878 in the SDRE. Steroids 76-91 ovalbumin (SERPINB14) Gallus gallus 166-175 8546694-6 1996 However, in the chicks treated with TAM and E2, OVA mRNA was degraded slowly over 48 h with a half-life of 24 h, suggesting that TAM does not inhibit E2-induced mRNA stabilization. Tamoxifen 36-39 ovalbumin (SERPINB14) Gallus gallus 48-51 7584852-0 1995 Induction of ovalbumin mRNA by ascorbic acid in primary cultures of tubular gland cells of the chicken oviduct. Ascorbic Acid 31-44 ovalbumin (SERPINB14) Gallus gallus 13-22 18623494-4 1995 Lysozyme and ovalbumin CD spectra in the corresponding GuHCl aqueous solutions revealed no changes in the higher order structures of the proteins. Cadmium 23-25 ovalbumin (SERPINB14) Gallus gallus 13-22 18623494-4 1995 Lysozyme and ovalbumin CD spectra in the corresponding GuHCl aqueous solutions revealed no changes in the higher order structures of the proteins. Guanidine 55-60 ovalbumin (SERPINB14) Gallus gallus 13-22 7584852-1 1995 The present study was conducted to investigate the effects of aging and medium supplements on steroid-induced ovalbumin mRNA in primary cultures of tubular gland cells from the chicken oviduct. Steroids 94-101 ovalbumin (SERPINB14) Gallus gallus 110-119 7584852-5 1995 The results indicated that the oviduct cells from immature chicks had clearer induction of ovalbumin mRNA by the steroid treatment than did those from laying hens. Steroids 113-120 ovalbumin (SERPINB14) Gallus gallus 91-100 7584852-7 1995 The drastic induction of ovalbumin mRNA by ascorbic acid supplementation was exerted only when the steroid hormones were present in the medium, implying that the effect of ascorbic acid may be auxiliary in steroid-induced transcription of the ovalbumin gene. Ascorbic Acid 43-56 ovalbumin (SERPINB14) Gallus gallus 25-34 7584852-7 1995 The drastic induction of ovalbumin mRNA by ascorbic acid supplementation was exerted only when the steroid hormones were present in the medium, implying that the effect of ascorbic acid may be auxiliary in steroid-induced transcription of the ovalbumin gene. Ascorbic Acid 43-56 ovalbumin (SERPINB14) Gallus gallus 243-252 7584852-7 1995 The drastic induction of ovalbumin mRNA by ascorbic acid supplementation was exerted only when the steroid hormones were present in the medium, implying that the effect of ascorbic acid may be auxiliary in steroid-induced transcription of the ovalbumin gene. Steroids 99-106 ovalbumin (SERPINB14) Gallus gallus 25-34 7584852-7 1995 The drastic induction of ovalbumin mRNA by ascorbic acid supplementation was exerted only when the steroid hormones were present in the medium, implying that the effect of ascorbic acid may be auxiliary in steroid-induced transcription of the ovalbumin gene. Ascorbic Acid 172-185 ovalbumin (SERPINB14) Gallus gallus 25-34 7584852-7 1995 The drastic induction of ovalbumin mRNA by ascorbic acid supplementation was exerted only when the steroid hormones were present in the medium, implying that the effect of ascorbic acid may be auxiliary in steroid-induced transcription of the ovalbumin gene. Ascorbic Acid 172-185 ovalbumin (SERPINB14) Gallus gallus 243-252 7584852-7 1995 The drastic induction of ovalbumin mRNA by ascorbic acid supplementation was exerted only when the steroid hormones were present in the medium, implying that the effect of ascorbic acid may be auxiliary in steroid-induced transcription of the ovalbumin gene. Steroids 206-213 ovalbumin (SERPINB14) Gallus gallus 25-34 7584852-7 1995 The drastic induction of ovalbumin mRNA by ascorbic acid supplementation was exerted only when the steroid hormones were present in the medium, implying that the effect of ascorbic acid may be auxiliary in steroid-induced transcription of the ovalbumin gene. Steroids 206-213 ovalbumin (SERPINB14) Gallus gallus 243-252 7665081-4 1995 SDS-PAGE and Western blotting analysis revealed that re-Ova immunoreacting with the egg ovalbumin antibody is not glycosylated. Sodium Dodecyl Sulfate 0-3 ovalbumin (SERPINB14) Gallus gallus 56-59 7490131-2 1995 Here we report that chicken ovalbumin (OVA) denatured by heat or sodium dodecyl sulphate (SDS) effectively induced CD8+ cytolytic T cells in vivo. Sodium Dodecyl Sulfate 65-88 ovalbumin (SERPINB14) Gallus gallus 28-37 7490131-2 1995 Here we report that chicken ovalbumin (OVA) denatured by heat or sodium dodecyl sulphate (SDS) effectively induced CD8+ cytolytic T cells in vivo. Sodium Dodecyl Sulfate 65-88 ovalbumin (SERPINB14) Gallus gallus 39-42 7490131-2 1995 Here we report that chicken ovalbumin (OVA) denatured by heat or sodium dodecyl sulphate (SDS) effectively induced CD8+ cytolytic T cells in vivo. Sodium Dodecyl Sulfate 90-93 ovalbumin (SERPINB14) Gallus gallus 28-37 7490131-2 1995 Here we report that chicken ovalbumin (OVA) denatured by heat or sodium dodecyl sulphate (SDS) effectively induced CD8+ cytolytic T cells in vivo. Sodium Dodecyl Sulfate 90-93 ovalbumin (SERPINB14) Gallus gallus 39-42 7490131-7 1995 In contrast, class I-restricted presentation of denatured OVA was sensitive to lysosomotropic agents (NH4Cl, vinblastine and leupeptin), indicating that endosomal-like compartments are involved in the presentation of denatured OVA. Ammonium Chloride 102-107 ovalbumin (SERPINB14) Gallus gallus 58-61 7490131-7 1995 In contrast, class I-restricted presentation of denatured OVA was sensitive to lysosomotropic agents (NH4Cl, vinblastine and leupeptin), indicating that endosomal-like compartments are involved in the presentation of denatured OVA. Ammonium Chloride 102-107 ovalbumin (SERPINB14) Gallus gallus 227-230 7490131-7 1995 In contrast, class I-restricted presentation of denatured OVA was sensitive to lysosomotropic agents (NH4Cl, vinblastine and leupeptin), indicating that endosomal-like compartments are involved in the presentation of denatured OVA. Vinblastine 109-120 ovalbumin (SERPINB14) Gallus gallus 58-61 7490131-7 1995 In contrast, class I-restricted presentation of denatured OVA was sensitive to lysosomotropic agents (NH4Cl, vinblastine and leupeptin), indicating that endosomal-like compartments are involved in the presentation of denatured OVA. Vinblastine 109-120 ovalbumin (SERPINB14) Gallus gallus 227-230 7490131-7 1995 In contrast, class I-restricted presentation of denatured OVA was sensitive to lysosomotropic agents (NH4Cl, vinblastine and leupeptin), indicating that endosomal-like compartments are involved in the presentation of denatured OVA. leupeptin 125-134 ovalbumin (SERPINB14) Gallus gallus 58-61 7490131-7 1995 In contrast, class I-restricted presentation of denatured OVA was sensitive to lysosomotropic agents (NH4Cl, vinblastine and leupeptin), indicating that endosomal-like compartments are involved in the presentation of denatured OVA. leupeptin 125-134 ovalbumin (SERPINB14) Gallus gallus 227-230 7490131-8 1995 Sensitization was inhibited at low temperature, yet took place in the presence of sucrose and in the absence of K+, indicating that denatured OVA enters the cell via fluid-phase endocytosis. Sucrose 82-89 ovalbumin (SERPINB14) Gallus gallus 142-145 7491104-2 1995 Two major regulatory elements reside in the chicken ovalbumin gene, a steroid-dependent regulatory element (SDRE, -892 to -780) and a negative regulatory element (NRE, -308 to -88). Steroids 70-77 ovalbumin (SERPINB14) Gallus gallus 52-61 7665081-4 1995 SDS-PAGE and Western blotting analysis revealed that re-Ova immunoreacting with the egg ovalbumin antibody is not glycosylated. Sodium Dodecyl Sulfate 0-3 ovalbumin (SERPINB14) Gallus gallus 88-97 7665081-5 1995 The re-Ova was purified by anion exchange chromatography into homogeneity, as evaluated by SDS-PAGE. Sodium Dodecyl Sulfate 91-94 ovalbumin (SERPINB14) Gallus gallus 7-10 8311248-1 1993 High-performance liquid chromatography (HPLC) interface to inductively coupled plasma mass spectrometry (ICPMS) has been applied for the first time to determine the sulfhydryl groups (SHs) in chicken ovalbumin (OVA) after their conversion into mercaptides with organic mercury compounds. mercaptides 244-255 ovalbumin (SERPINB14) Gallus gallus 200-209 8311248-1 1993 High-performance liquid chromatography (HPLC) interface to inductively coupled plasma mass spectrometry (ICPMS) has been applied for the first time to determine the sulfhydryl groups (SHs) in chicken ovalbumin (OVA) after their conversion into mercaptides with organic mercury compounds. mercaptides 244-255 ovalbumin (SERPINB14) Gallus gallus 211-214 8311248-1 1993 High-performance liquid chromatography (HPLC) interface to inductively coupled plasma mass spectrometry (ICPMS) has been applied for the first time to determine the sulfhydryl groups (SHs) in chicken ovalbumin (OVA) after their conversion into mercaptides with organic mercury compounds. Mercury 269-276 ovalbumin (SERPINB14) Gallus gallus 200-209 8311248-1 1993 High-performance liquid chromatography (HPLC) interface to inductively coupled plasma mass spectrometry (ICPMS) has been applied for the first time to determine the sulfhydryl groups (SHs) in chicken ovalbumin (OVA) after their conversion into mercaptides with organic mercury compounds. Mercury 269-276 ovalbumin (SERPINB14) Gallus gallus 211-214 8311248-3 1993 The rates of reaction of five mercaptide-forming reagents with SHs in OVA were in the order ethylmercuric chloride > mersalyl acid > p-chloromercuribenzoate > p-(chloromercuri)benzenesulfonic acid > fluorescein mercuric acetate. mercaptide 30-40 ovalbumin (SERPINB14) Gallus gallus 70-73 8311248-3 1993 The rates of reaction of five mercaptide-forming reagents with SHs in OVA were in the order ethylmercuric chloride > mersalyl acid > p-chloromercuribenzoate > p-(chloromercuri)benzenesulfonic acid > fluorescein mercuric acetate. Ethylmercuric Chloride 92-114 ovalbumin (SERPINB14) Gallus gallus 70-73 8311248-3 1993 The rates of reaction of five mercaptide-forming reagents with SHs in OVA were in the order ethylmercuric chloride > mersalyl acid > p-chloromercuribenzoate > p-(chloromercuri)benzenesulfonic acid > fluorescein mercuric acetate. Mersalyl 120-133 ovalbumin (SERPINB14) Gallus gallus 70-73 8311248-3 1993 The rates of reaction of five mercaptide-forming reagents with SHs in OVA were in the order ethylmercuric chloride > mersalyl acid > p-chloromercuribenzoate > p-(chloromercuri)benzenesulfonic acid > fluorescein mercuric acetate. p-chloromercuribenzoate 139-162 ovalbumin (SERPINB14) Gallus gallus 70-73 8311248-3 1993 The rates of reaction of five mercaptide-forming reagents with SHs in OVA were in the order ethylmercuric chloride > mersalyl acid > p-chloromercuribenzoate > p-(chloromercuri)benzenesulfonic acid > fluorescein mercuric acetate. 4-Chloromercuribenzenesulfonate 168-205 ovalbumin (SERPINB14) Gallus gallus 70-73 8311248-3 1993 The rates of reaction of five mercaptide-forming reagents with SHs in OVA were in the order ethylmercuric chloride > mersalyl acid > p-chloromercuribenzoate > p-(chloromercuri)benzenesulfonic acid > fluorescein mercuric acetate. fluorescein mercuric acetate 211-239 ovalbumin (SERPINB14) Gallus gallus 70-73 8514758-0 1993 A complex array of double-stranded and single-stranded DNA-binding proteins mediates induction of the ovalbumin gene by steroid hormones. Steroids 120-136 ovalbumin (SERPINB14) Gallus gallus 102-111 8404652-6 1993 As expected, ovalbumin gene transcription is increased only after a lag period of 4 h following Pg treatment. Progesterone 96-98 ovalbumin (SERPINB14) Gallus gallus 13-22 8399219-3 1993 In analogy with hen egg ovalbumin, where the same proteases all cleaved the polypeptide chain very specifically around Ala-352, Ala-323 of Mib-CK may be located in an exposed surface loop that is sensitive to protease attack. Alanine 119-122 ovalbumin (SERPINB14) Gallus gallus 24-33 8399219-3 1993 In analogy with hen egg ovalbumin, where the same proteases all cleaved the polypeptide chain very specifically around Ala-352, Ala-323 of Mib-CK may be located in an exposed surface loop that is sensitive to protease attack. Alanine 128-131 ovalbumin (SERPINB14) Gallus gallus 24-33 8113098-0 1993 Ovalbumin-like immunoreactivity detected in chicken sensory neurons by antibodies to aldehyde-treated ovalbumin. Aldehydes 85-93 ovalbumin (SERPINB14) Gallus gallus 0-9 8113098-0 1993 Ovalbumin-like immunoreactivity detected in chicken sensory neurons by antibodies to aldehyde-treated ovalbumin. Aldehydes 85-93 ovalbumin (SERPINB14) Gallus gallus 102-111 8113098-2 1993 Ovalbumin-like immunoreactivity has been identified in a subpopulation of chicken dorsal root ganglion neurons by the generation of antibodies to aldehyde-conjugated ovalbumin but not by the antibodies to native ovalbumin, although both antibodies recognize the much higher concentrations of ovalbumin in sections of the oviduct. Aldehydes 146-154 ovalbumin (SERPINB14) Gallus gallus 0-9 8113098-2 1993 Ovalbumin-like immunoreactivity has been identified in a subpopulation of chicken dorsal root ganglion neurons by the generation of antibodies to aldehyde-conjugated ovalbumin but not by the antibodies to native ovalbumin, although both antibodies recognize the much higher concentrations of ovalbumin in sections of the oviduct. Aldehydes 146-154 ovalbumin (SERPINB14) Gallus gallus 166-175 8514758-1 1993 The transcriptional induction of the chicken ovalbumin gene by steroid hormones is abolished by inhibitors of protein synthesis such as cycloheximide, suggesting that a labile protein mediates this process. Steroids 63-79 ovalbumin (SERPINB14) Gallus gallus 45-54 8514758-1 1993 The transcriptional induction of the chicken ovalbumin gene by steroid hormones is abolished by inhibitors of protein synthesis such as cycloheximide, suggesting that a labile protein mediates this process. Cycloheximide 136-149 ovalbumin (SERPINB14) Gallus gallus 45-54 1279383-0 1992 Regulation of expression of the chicken ovalbumin gene: interactions between steroid hormones and second messenger systems. Steroids 77-93 ovalbumin (SERPINB14) Gallus gallus 40-49 1291045-1 1992 Resolution and structural characterization of ovalbumin glycans. Polysaccharides 56-63 ovalbumin (SERPINB14) Gallus gallus 46-55 1291045-2 1992 The microheterogeneous mixture of fluoresceinated glycopeptides (FGPs) obtained from the single site of glycosylation of chicken ovalbumin was resolved by a combination of discontinuous electrophoresis in a high-density poly(acrylamide) gel (PAGE) for sizing, in conjunction with borate-PAGE. fluoresceinated glycopeptides 34-63 ovalbumin (SERPINB14) Gallus gallus 129-138 1291045-2 1992 The microheterogeneous mixture of fluoresceinated glycopeptides (FGPs) obtained from the single site of glycosylation of chicken ovalbumin was resolved by a combination of discontinuous electrophoresis in a high-density poly(acrylamide) gel (PAGE) for sizing, in conjunction with borate-PAGE. polyacrylamide 220-236 ovalbumin (SERPINB14) Gallus gallus 129-138 1279383-7 1992 The results indicate that phorbol 12-myristilate 13-acetate causes a dramatic destabilization of ovalbumin message, resulting in a reduction in ovalbumin mRNA levels. phorbol 12-myristilate 13-acetate 26-59 ovalbumin (SERPINB14) Gallus gallus 97-106 1279383-7 1992 The results indicate that phorbol 12-myristilate 13-acetate causes a dramatic destabilization of ovalbumin message, resulting in a reduction in ovalbumin mRNA levels. phorbol 12-myristilate 13-acetate 26-59 ovalbumin (SERPINB14) Gallus gallus 144-153 1279383-8 1992 In contrast, the activators of the PKA system can substitute for insulin and, thereby, increase expression of the ovalbumin gene synergistically with the steroids. Steroids 154-162 ovalbumin (SERPINB14) Gallus gallus 114-123 1279383-10 1992 Thus, in chicken oviduct cell cultures, the PKA and PKC signal transduction pathways act in opposing ways to modulate the steroid-induced expression of the ovalbumin gene. Steroids 122-129 ovalbumin (SERPINB14) Gallus gallus 156-165 2332444-0 1990 The steroid-dependent regulatory element in the ovalbumin gene does not function as a typical steroid response element. Steroids 4-11 ovalbumin (SERPINB14) Gallus gallus 48-57 1546973-1 1992 The equilibrium binding of the apolar fluorescent dye 1-anilinonaphthalene-8-sulphonate (ANS) to bacteriorhodopsin, BSA, chicken egg lysozyme, ovalbumin, porcine somatotrophin (PST) and bovine pancreatic ribonuclease (RNAase) was quantitatively evaluated using Scatchard- and Klotz-plot analyses. 1-anilinonaphthalene-8-sulphonate 54-87 ovalbumin (SERPINB14) Gallus gallus 143-152 1694878-0 1990 Different roles for thiol and aspartyl proteases in antigen presentation of ovalbumin. Sulfhydryl Compounds 20-25 ovalbumin (SERPINB14) Gallus gallus 76-85 2141621-2 1990 Tryptic digest and cyanogen bromide fragments of chicken ovalbumin and synthetic peptides of ovalbumin (323-339) and influenza virus (NP 365-380) were used to generate CTL and T helper lines from unprimed T cells. Cyanogen Bromide 19-35 ovalbumin (SERPINB14) Gallus gallus 57-66 1562535-0 1992 In situ hybridization of ovalbumin mRNA in the chick oviduct reveals target cell specificity for estrogen and progesterone. Progesterone 110-122 ovalbumin (SERPINB14) Gallus gallus 25-34 1562535-1 1992 An in situ hybridization method using paraffin-embedded sections was used to characterize the chicken oviduct cells synthesizing ovalbumin mRNA due to the action of estrogen and progesterone. Paraffin 38-46 ovalbumin (SERPINB14) Gallus gallus 129-138 1562535-1 1992 An in situ hybridization method using paraffin-embedded sections was used to characterize the chicken oviduct cells synthesizing ovalbumin mRNA due to the action of estrogen and progesterone. Progesterone 178-190 ovalbumin (SERPINB14) Gallus gallus 129-138 1562535-6 1992 Administration of progesterone induced the expression of ovalbumin mRNA in the surface epithelial cells. Progesterone 18-30 ovalbumin (SERPINB14) Gallus gallus 57-66 1562535-9 1992 We conclude that the expression of ovalbumin in the surface epithelial cells and in the tubular gland cells is specific for progesterone and estrogen, respectively. Progesterone 124-136 ovalbumin (SERPINB14) Gallus gallus 35-44 1450986-16 1992 Butyrate inhibits the induction of proteins, including enzymes, by steroid hormones as has been shown for the induction of tyrosine aminotransferase by glucocorticoids, of ovalbumin and transferrin by estradiol in chick oviduct. Butyrates 0-8 ovalbumin (SERPINB14) Gallus gallus 172-181 1450986-16 1992 Butyrate inhibits the induction of proteins, including enzymes, by steroid hormones as has been shown for the induction of tyrosine aminotransferase by glucocorticoids, of ovalbumin and transferrin by estradiol in chick oviduct. Steroids 67-83 ovalbumin (SERPINB14) Gallus gallus 172-181 2037595-0 1991 A protein with a binding specificity similar to NF-kappa B binds to a steroid-dependent regulatory element in the ovalbumin gene. Steroids 70-77 ovalbumin (SERPINB14) Gallus gallus 114-123 2037595-1 1991 The chicken ovalbumin gene is regulated at the level of transcription by four classes of steroid hormones. Steroids 89-105 ovalbumin (SERPINB14) Gallus gallus 12-21 2037595-4 1991 Fusion genes containing the mutant ovalbumin 5"-flanking sequences linked to the chloramphenicol acetyltransferase structural gene (CAT) were transfected into steroid-responsive primary oviduct cells. Steroids 159-166 ovalbumin (SERPINB14) Gallus gallus 35-44 2037595-12 1991 These data suggest that a protein binding to sequences in the SDRE that are similar to an NF-kappa B-binding site participates in the steroid-mediated increase in transcription of the chicken ovalbumin gene. Steroids 134-141 ovalbumin (SERPINB14) Gallus gallus 192-201 2332444-1 1990 Transcription of the chicken ovalbumin gene is induced both in vivo and in vitro by four classes of steroid hormones. Steroids 100-116 ovalbumin (SERPINB14) Gallus gallus 29-38 2332444-4 1990 When the ovalbumin-chloramphenicol acetyltransferase fusion genes were transfected into steroid-responsive primary oviduct cells, mutants lacking sequences between -900 and -732 were no longer responsive to estrogen, corticosterone, progesterone, or dihydrotestosterone. Steroids 88-95 ovalbumin (SERPINB14) Gallus gallus 9-18 2332444-4 1990 When the ovalbumin-chloramphenicol acetyltransferase fusion genes were transfected into steroid-responsive primary oviduct cells, mutants lacking sequences between -900 and -732 were no longer responsive to estrogen, corticosterone, progesterone, or dihydrotestosterone. Corticosterone 217-231 ovalbumin (SERPINB14) Gallus gallus 9-18 2332444-4 1990 When the ovalbumin-chloramphenicol acetyltransferase fusion genes were transfected into steroid-responsive primary oviduct cells, mutants lacking sequences between -900 and -732 were no longer responsive to estrogen, corticosterone, progesterone, or dihydrotestosterone. Progesterone 233-245 ovalbumin (SERPINB14) Gallus gallus 9-18 2332444-4 1990 When the ovalbumin-chloramphenicol acetyltransferase fusion genes were transfected into steroid-responsive primary oviduct cells, mutants lacking sequences between -900 and -732 were no longer responsive to estrogen, corticosterone, progesterone, or dihydrotestosterone. Dihydrotestosterone 250-269 ovalbumin (SERPINB14) Gallus gallus 9-18 2332444-10 1990 These data indicate that induction of the ovalbumin gene by steroid hormones requires complex interactions involving both the SDRE and the negative regulatory element. Steroids 60-76 ovalbumin (SERPINB14) Gallus gallus 42-51 2404743-11 1990 After 2-week stimulation with DES, avidin was expressed predominantly by cells of the basal layer of pseudostratified surface epithelium, and ovalbumin mainly by tubular glands and cells of the luminal layer of surface epithelium. Diethylstilbestrol 30-33 ovalbumin (SERPINB14) Gallus gallus 142-151 2139364-4 1990 In mice treated with 400 ng of PT and 1 mg of chicken egg albumin (EA), the percentage of these cells increased, 14 days after immunization, to an average value of 31.1 +/- 2.2%. Platinum 31-33 ovalbumin (SERPINB14) Gallus gallus 67-69 16667406-5 1990 Both activities comigrated with chicken ovalbumin during gel filtration through Sephacryl S-200, indicating a native molecular mass of 45 kilodaltons. sephacryl S 200 80-95 ovalbumin (SERPINB14) Gallus gallus 40-49