PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 30430898-7 2019 Taken together, our study indicates that Smad2/3/SphK1/S1P/Notch3 pathway mediates TGF-beta1-induced PASMC proliferation and suggests this pathway as a potential therapeutic target in the prevention and treatment of pulmonary hypertension. pasmc 101-106 sphingosine kinase 1 Homo sapiens 49-54 30430898-4 2019 Here, we demonstrated that TGF-beta1 significantly upregulated SphK1 expression and S1P production by promoting the phosphorylation of Smad2/3 in PASMC. pasmc 146-151 sphingosine kinase 1 Homo sapiens 63-68 30154249-9 2019 Inhibition of the FASN/SPHK/S1P axis was accompanied by decreased activation of p-MET, p-FAK, and p-PAX. p-pax 98-103 sphingosine kinase 1 Homo sapiens 23-27 30430898-5 2019 Further study indicated that SphK1/S1P pathway mediated TGF-beta1-induced Notch3 activation in PASMC. pasmc 95-100 sphingosine kinase 1 Homo sapiens 29-34 29940125-0 2018 Quercetin ameliorates pulmonary fibrosis by inhibiting SphK1/S1P signaling. Quercetin 0-9 sphingosine kinase 1 Homo sapiens 55-60 29940125-6 2018 The bleomycin or TGF-beta administration caused the increase of sphingosine-1-phosphate (S1P) level in pulmonary tissue and HELF cells, as well as its activation-required kinase, sphingosine kinase 1 (SphK1), and its degradation enzyme, sphinogosine-1-phosphate lyase (S1PL). Bleomycin 4-13 sphingosine kinase 1 Homo sapiens 179-199 29940125-6 2018 The bleomycin or TGF-beta administration caused the increase of sphingosine-1-phosphate (S1P) level in pulmonary tissue and HELF cells, as well as its activation-required kinase, sphingosine kinase 1 (SphK1), and its degradation enzyme, sphinogosine-1-phosphate lyase (S1PL). Bleomycin 4-13 sphingosine kinase 1 Homo sapiens 201-206 29940125-7 2018 However, the increase of S1P, SphK1, and S1PL was attenuated by application of quercetin. Quercetin 79-88 sphingosine kinase 1 Homo sapiens 30-35 29940125-8 2018 In addition, the effect of quercetin on fibrosis was abolished by the ectopic expression of SphK1. Quercetin 27-36 sphingosine kinase 1 Homo sapiens 92-97 29940125-10 2018 In summary, we demonstrated that quercetin ameliorated pulmonary fibrosis in vivo and in vitro by inhibiting SphK1/S1P signaling. Quercetin 33-42 sphingosine kinase 1 Homo sapiens 109-114 30315000-0 2018 Sphingosine kinase 1 activation by estrogen receptor alpha36 contributes to tamoxifen resistance in breast cancer. Tamoxifen 76-85 sphingosine kinase 1 Homo sapiens 0-20 30315000-3 2018 E2 activates sphingosine kinase 1 (SphK1), increasing sphingosine-1-phosphate (S1P) that binds to its receptors, leading to important breast cancer signaling. sphingosine 1-phosphate 54-77 sphingosine kinase 1 Homo sapiens 13-33 30315000-3 2018 E2 activates sphingosine kinase 1 (SphK1), increasing sphingosine-1-phosphate (S1P) that binds to its receptors, leading to important breast cancer signaling. sphingosine 1-phosphate 54-77 sphingosine kinase 1 Homo sapiens 35-40 30315000-6 2018 Tamoxifen, the first-line endocrine therapy for breast cancer, is an antagonist of ERalpha66, but an agonist of ERalpha36, and, like E2, activates SphK1 and markedly increases secretion of S1P. Tamoxifen 0-9 sphingosine kinase 1 Homo sapiens 147-152 30315000-8 2018 We found that tamoxifen resistance correlated with increased SphK1 and ERalpha36 expression in tamoxifen-resistant breast cancer cells, in patient-derived xenografts, and in endocrine-resistant breast cancer patients. Tamoxifen 14-23 sphingosine kinase 1 Homo sapiens 61-66 30315000-8 2018 We found that tamoxifen resistance correlated with increased SphK1 and ERalpha36 expression in tamoxifen-resistant breast cancer cells, in patient-derived xenografts, and in endocrine-resistant breast cancer patients. Tamoxifen 95-104 sphingosine kinase 1 Homo sapiens 61-66 30185808-3 2018 The kinases SphK1 and SphK2 (SphKs) phosphorilate Sphingosine (Sph) to S1P and several phosphatases revert S1P to sphingosine, thus assuring a balanced pool that can be depleted by a Sphingosine lyase in hexadecenal compounds and aldehydes. Sphingosine 50-61 sphingosine kinase 1 Homo sapiens 12-17 30281914-10 2018 We found elevated SPHK1 expression in GC tissues and cells, along with an increased concentration of SPHK1-generated sphingosine-1-phosphate (S1P) in both GC serum and tissue. sphingosine 1-phosphate 117-140 sphingosine kinase 1 Homo sapiens 101-106 30148701-6 2018 However, blockade of ceramide to S1P metabolic conversion using a specific inhibitor of SPHK1 attenuated the expected Rb1-mediated increase in KC migration. Ceramides 21-29 sphingosine kinase 1 Homo sapiens 88-93 30277602-7 2018 Notably, IS20 blocked not only MPTP-induced reductions in the A2 phenotypic markers SPHK1 and SCL10a6 but also elevation of the of A1 marker GBP2. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 31-35 sphingosine kinase 1 Homo sapiens 84-89 30138818-0 2018 Sphingosine kinase 1/sphingosine-1-phosphate receptors dependent signalling in neurodegenerative diseases. Phosphates 35-44 sphingosine kinase 1 Homo sapiens 0-20 30524898-0 2018 Sphingosine kinase 1 overexpression contributes to sunitinib resistance in clear cell renal cell carcinoma. Sunitinib 51-60 sphingosine kinase 1 Homo sapiens 0-20 30524898-1 2018 Sphingosine kinase 1 (SphK1) is the major source of the bioactive lipid and GPCR agonist sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 89-112 sphingosine kinase 1 Homo sapiens 0-20 30524898-1 2018 Sphingosine kinase 1 (SphK1) is the major source of the bioactive lipid and GPCR agonist sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 89-112 sphingosine kinase 1 Homo sapiens 22-27 30524898-8 2018 Our results unraveled that increased SphK1 kinase activation defines an important mechanism for sunitinib resistance, therefore contributes to tumour development and represents therapeutic targets for ccRCC. Sunitinib 96-105 sphingosine kinase 1 Homo sapiens 37-42 30591148-2 2018 One enzyme is the critical lipid kinase sphingosine kinase 1 (SK1), which produces the potent and pleiotropic signaling lipid, sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 127-150 sphingosine kinase 1 Homo sapiens 40-60 30591148-2 2018 One enzyme is the critical lipid kinase sphingosine kinase 1 (SK1), which produces the potent and pleiotropic signaling lipid, sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 127-150 sphingosine kinase 1 Homo sapiens 62-65 30591148-2 2018 One enzyme is the critical lipid kinase sphingosine kinase 1 (SK1), which produces the potent and pleiotropic signaling lipid, sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 152-155 sphingosine kinase 1 Homo sapiens 40-60 30591148-2 2018 One enzyme is the critical lipid kinase sphingosine kinase 1 (SK1), which produces the potent and pleiotropic signaling lipid, sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 152-155 sphingosine kinase 1 Homo sapiens 62-65 30278948-2 2018 Sphingosine-1-phosphate (S1P) is a bioactive lipid mediator that is generated by sphingosine kinase 1 (SphK1) and is known to play an important role in inflammation and cancer progression. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 81-101 30278948-2 2018 Sphingosine-1-phosphate (S1P) is a bioactive lipid mediator that is generated by sphingosine kinase 1 (SphK1) and is known to play an important role in inflammation and cancer progression. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 103-108 30092415-10 2018 Mechanistically, the anti-leukemia activity of PG was mediated by its ability to repress SphK1 and thus modulate ceramide-S1P rheostat. physcion 8-O-glucopyranoside 47-49 sphingosine kinase 1 Homo sapiens 89-94 30310531-3 2018 Particularly, sphingosine kinase 1 (SphK1) and its product sphingosine-1-phosphate (S1P) operate as mediators and facilitators of proliferation-linked signaling. sphingosine 1-phosphate 59-82 sphingosine kinase 1 Homo sapiens 14-34 30310531-3 2018 Particularly, sphingosine kinase 1 (SphK1) and its product sphingosine-1-phosphate (S1P) operate as mediators and facilitators of proliferation-linked signaling. sphingosine 1-phosphate 59-82 sphingosine kinase 1 Homo sapiens 36-41 30185808-3 2018 The kinases SphK1 and SphK2 (SphKs) phosphorilate Sphingosine (Sph) to S1P and several phosphatases revert S1P to sphingosine, thus assuring a balanced pool that can be depleted by a Sphingosine lyase in hexadecenal compounds and aldehydes. Sphingosine 114-125 sphingosine kinase 1 Homo sapiens 12-17 30185808-3 2018 The kinases SphK1 and SphK2 (SphKs) phosphorilate Sphingosine (Sph) to S1P and several phosphatases revert S1P to sphingosine, thus assuring a balanced pool that can be depleted by a Sphingosine lyase in hexadecenal compounds and aldehydes. Aldehydes 230-239 sphingosine kinase 1 Homo sapiens 12-17 29956748-2 2018 Previous studies have shown that a critical enzyme involved in the sphingomyelin cycle, sphingosine kinase 1 (SPHK1), is implicated in inflammatory processes and contributes to chronic neuroinflammation. Sphingomyelins 67-80 sphingosine kinase 1 Homo sapiens 88-108 30044952-0 2018 Synthesis of dansyl labeled sphingosine kinase 1 inhibitor. 1-myristoyl-2-(12-((5-dimethylamino-1-naphthalenesulfonyl)amino)dodecanoyl)-sn-glycero-3-phosphocholine 13-19 sphingosine kinase 1 Homo sapiens 28-48 30044952-1 2018 PF-543 is a non-sphingosine analogue with inhibitory effect against SK1, based on a Ki of 4.3 nM and 130-fold selectivity for SK1 over SK2. PF-543 0-6 sphingosine kinase 1 Homo sapiens 68-71 30044952-1 2018 PF-543 is a non-sphingosine analogue with inhibitory effect against SK1, based on a Ki of 4.3 nM and 130-fold selectivity for SK1 over SK2. PF-543 0-6 sphingosine kinase 1 Homo sapiens 126-129 30044952-5 2018 A docking study of SK1 and SK1 inhibitory activity confirmed the structural similarity between the synthetic dansyl-PF-543 and PF-543. dansyl-pf 109-118 sphingosine kinase 1 Homo sapiens 19-22 30044952-5 2018 A docking study of SK1 and SK1 inhibitory activity confirmed the structural similarity between the synthetic dansyl-PF-543 and PF-543. dansyl-pf 109-118 sphingosine kinase 1 Homo sapiens 27-30 30005204-8 2018 One of the most important genes identified by microarray analysis was sphingosine kinase-1 (SPHK1), which is a key molecule in sphingolipid metabolism. Sphingolipids 127-139 sphingosine kinase 1 Homo sapiens 70-90 30005204-8 2018 One of the most important genes identified by microarray analysis was sphingosine kinase-1 (SPHK1), which is a key molecule in sphingolipid metabolism. Sphingolipids 127-139 sphingosine kinase 1 Homo sapiens 92-97 29956748-2 2018 Previous studies have shown that a critical enzyme involved in the sphingomyelin cycle, sphingosine kinase 1 (SPHK1), is implicated in inflammatory processes and contributes to chronic neuroinflammation. Sphingomyelins 67-80 sphingosine kinase 1 Homo sapiens 110-115 30005204-9 2018 SPHK1 knock-down drastically reduced ROS scavenging efficiency as well as expression levels of inflammation-related proteins in PMA-treated HaCaT cells. Reactive Oxygen Species 37-40 sphingosine kinase 1 Homo sapiens 0-5 32002951-0 2018 Sphingosine kinase 1/sphingosine-1-phosphate receptors dependent signalling in neurodegenerative diseases. Phosphates 35-44 sphingosine kinase 1 Homo sapiens 0-20 29971548-0 2018 Theoretical models to predict the inhibitory effect of ligands of sphingosine kinase 1 using QTAIM calculations and hydrogen bond dynamic propensity analysis. Hydrogen 116-124 sphingosine kinase 1 Homo sapiens 66-86 29728804-7 2018 As for results, the amount of sphingosine kinase (SPHK)-1 and SPHK-2 in RAW264.7 macrophages increased in the presence of titanium particles in a time-dependent manner. Titanium 122-130 sphingosine kinase 1 Homo sapiens 30-57 30018456-2 2018 Recently, sphingosine-1-phosphate (S1P), a bioactive lipid mediator generated by sphingosine kinase 1 (SPHK1), has been shown to play an important role in lymphangiogenesis and lymph node metastasis in several types of cancer. sphingosine 1-phosphate 10-33 sphingosine kinase 1 Homo sapiens 81-101 30018456-2 2018 Recently, sphingosine-1-phosphate (S1P), a bioactive lipid mediator generated by sphingosine kinase 1 (SPHK1), has been shown to play an important role in lymphangiogenesis and lymph node metastasis in several types of cancer. sphingosine 1-phosphate 10-33 sphingosine kinase 1 Homo sapiens 103-108 30018456-4 2018 Here we found that S1P is elevated in BTC with the activation of ceramide-synthetic pathways, suggesting that BTC utilizes SPHK1 to promote lymphatic metastasis. Ceramides 65-73 sphingosine kinase 1 Homo sapiens 123-128 32002971-10 2018 Mechanistically, the anti-leukemia activity of PG was mediated by its ability to repress SphK1 and thus modulate ceramide-S1P rheostat. physcione 47-49 sphingosine kinase 1 Homo sapiens 89-94 29901156-5 2018 Furthermore, overexpression of miR-125b significantly promoted amyloid precursor protein and beta-secretase 1 expression and beta-amyloid peptide production, and suppressed sphingosine kinase 1 (SphK1) protein expression in vitro. mir-125b 31-39 sphingosine kinase 1 Homo sapiens 173-193 29901156-5 2018 Furthermore, overexpression of miR-125b significantly promoted amyloid precursor protein and beta-secretase 1 expression and beta-amyloid peptide production, and suppressed sphingosine kinase 1 (SphK1) protein expression in vitro. mir-125b 31-39 sphingosine kinase 1 Homo sapiens 195-200 29901156-6 2018 These findings suggested that miR-125b may regulate AD, and neuronal cell growth and apoptosis, via the regulation of inflammatory factors and oxidative stress by SphK1; therefore, miR-125b may be involved in the development of AD. mir-125b 30-38 sphingosine kinase 1 Homo sapiens 163-168 29901156-6 2018 These findings suggested that miR-125b may regulate AD, and neuronal cell growth and apoptosis, via the regulation of inflammatory factors and oxidative stress by SphK1; therefore, miR-125b may be involved in the development of AD. mir-125b 181-189 sphingosine kinase 1 Homo sapiens 163-168 29950445-4 2018 This results in an imbalance in the control of E2F-dependent targets such as SPHK1, which is derepressed and drives resistance to anthracyclines in HNSCC. Anthracyclines 130-144 sphingosine kinase 1 Homo sapiens 77-82 29844803-0 2018 Sphingosine kinase 1 overexpression is associated with poor prognosis and oxaliplatin resistance in hepatocellular carcinoma. Oxaliplatin 74-85 sphingosine kinase 1 Homo sapiens 0-20 29805524-13 2018 Furthermore, inhibition of SPHK1 by siRNA or treatment with SPHK1 inhibitor 5C sensitized thyroid carcinoma to cisplatin and doxorubicin. Cisplatin 111-120 sphingosine kinase 1 Homo sapiens 27-32 29805524-13 2018 Furthermore, inhibition of SPHK1 by siRNA or treatment with SPHK1 inhibitor 5C sensitized thyroid carcinoma to cisplatin and doxorubicin. Cisplatin 111-120 sphingosine kinase 1 Homo sapiens 60-65 29844803-2 2018 However, the role of SphK1 in the resistance of hepatocellular carcinoma (HCC) to oxaliplatin remains unclear. Oxaliplatin 82-93 sphingosine kinase 1 Homo sapiens 21-26 29805524-13 2018 Furthermore, inhibition of SPHK1 by siRNA or treatment with SPHK1 inhibitor 5C sensitized thyroid carcinoma to cisplatin and doxorubicin. Doxorubicin 125-136 sphingosine kinase 1 Homo sapiens 27-32 29805524-13 2018 Furthermore, inhibition of SPHK1 by siRNA or treatment with SPHK1 inhibitor 5C sensitized thyroid carcinoma to cisplatin and doxorubicin. Doxorubicin 125-136 sphingosine kinase 1 Homo sapiens 60-65 29844803-5 2018 Furthermore, SphK1 expression levels and activity were analyzed in a series of HCC cell lines and they were both demonstrated to be associated with resistance to oxaliplatin. Oxaliplatin 162-173 sphingosine kinase 1 Homo sapiens 13-18 29844803-6 2018 Conversely, the knockdown of SphK1 protein expression resulted in decreased oxaliplatin resistance in SK-Hep1 and HCCLM3 cell lines. Oxaliplatin 76-87 sphingosine kinase 1 Homo sapiens 29-34 29844803-7 2018 In addition, the results of the current study demonstrated that the downregulation of SphK1 decreased the levels of phosphorylated AKT serine/threonine kinase (Akt) and glycogen synthase kinase-3beta (GSK3beta), suggesting that SphK1 promotes oxaliplatin resistance of HCC cells via modulation of the Akt/GSK3beta signaling pathway. Oxaliplatin 243-254 sphingosine kinase 1 Homo sapiens 86-91 29844803-8 2018 To the best of our knowledge, the present study is the first to report that SphK1 is associated with poor prognosis and oxaliplatin resistance in HCC. Oxaliplatin 120-131 sphingosine kinase 1 Homo sapiens 76-81 29955024-1 2018 Sphingosine 1-phosphate (S1P) is a bioactive phosphorylated product of sphingosine catalyzed by sphingosine kinase (SphK) and implicated in diverse cellular functions including vesicular trafficking. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 96-114 29377379-2 2018 Among these regulators, the bioactive lipid sphingosine-1-phosphate (S1P), which is synthesized by sphingosine kinase 1/2 (SPHK1/2), has recently been recognized to play a role in immunity and bone remodeling through its receptor sphingosine-1-phosphate receptor 1 (S1PR1). sphingosine 1-phosphate 44-67 sphingosine kinase 1 Homo sapiens 99-121 29377379-2 2018 Among these regulators, the bioactive lipid sphingosine-1-phosphate (S1P), which is synthesized by sphingosine kinase 1/2 (SPHK1/2), has recently been recognized to play a role in immunity and bone remodeling through its receptor sphingosine-1-phosphate receptor 1 (S1PR1). sphingosine 1-phosphate 44-67 sphingosine kinase 1 Homo sapiens 123-130 29928348-1 2018 Sphingosine kinase 1 (SphK1) is a master kinase that catalyzes the synthesis of sphingosine 1 phosphate and participates in the regulation of cell proliferation and autophagy. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 0-20 29928348-1 2018 Sphingosine kinase 1 (SphK1) is a master kinase that catalyzes the synthesis of sphingosine 1 phosphate and participates in the regulation of cell proliferation and autophagy. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 22-27 29370930-2 2018 Previous studies suggested that sphingosine-1-phosphate produced by sphingosine kinase 1, which is activated by phosphorylation, plays important roles in the progression of disease and metastasis. sphingosine 1-phosphate 32-55 sphingosine kinase 1 Homo sapiens 68-88 29370930-3 2018 The association between phospho-sphingosine-1-phosphate produced by sphingosine kinase 1 and clinical parameters in human gastric cancer have not been fully investigated to date. phospho-sphingosine-1-phosphate 24-55 sphingosine kinase 1 Homo sapiens 68-88 29955024-1 2018 Sphingosine 1-phosphate (S1P) is a bioactive phosphorylated product of sphingosine catalyzed by sphingosine kinase (SphK) and implicated in diverse cellular functions including vesicular trafficking. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 116-120 29955024-1 2018 Sphingosine 1-phosphate (S1P) is a bioactive phosphorylated product of sphingosine catalyzed by sphingosine kinase (SphK) and implicated in diverse cellular functions including vesicular trafficking. Sphingosine 71-82 sphingosine kinase 1 Homo sapiens 96-114 29955024-1 2018 Sphingosine 1-phosphate (S1P) is a bioactive phosphorylated product of sphingosine catalyzed by sphingosine kinase (SphK) and implicated in diverse cellular functions including vesicular trafficking. Sphingosine 71-82 sphingosine kinase 1 Homo sapiens 116-120 29955024-6 2018 When cells were treated with N,N-dimethylsphingosine (DMS), one of the most frequently used inhibitors for SphK, cargo contents in purified exosomes were enhanced unexpectedly. N,N-dimethylsphingosine 29-52 sphingosine kinase 1 Homo sapiens 107-111 29955024-6 2018 When cells were treated with N,N-dimethylsphingosine (DMS), one of the most frequently used inhibitors for SphK, cargo contents in purified exosomes were enhanced unexpectedly. N,N-dimethylsphingosine 54-57 sphingosine kinase 1 Homo sapiens 107-111 29167124-5 2018 Our results demonstrate that miR-1 is downregulated by hypoxia in PASMCs and can directly inhibit SphK1 expression. pasmcs 66-72 sphingosine kinase 1 Homo sapiens 98-103 28942351-1 2018 The bioactive lipid, sphingosine 1-phosphate (S1P) is produced by phosphorylation of sphingosine and this is catalysed by two sphingosine kinase isoforms (SK1 and SK2). sphingosine 1-phosphate 21-44 sphingosine kinase 1 Homo sapiens 155-158 28942351-1 2018 The bioactive lipid, sphingosine 1-phosphate (S1P) is produced by phosphorylation of sphingosine and this is catalysed by two sphingosine kinase isoforms (SK1 and SK2). sphingosine 1-phosphate 46-49 sphingosine kinase 1 Homo sapiens 155-158 28942351-1 2018 The bioactive lipid, sphingosine 1-phosphate (S1P) is produced by phosphorylation of sphingosine and this is catalysed by two sphingosine kinase isoforms (SK1 and SK2). Sphingosine 21-32 sphingosine kinase 1 Homo sapiens 155-158 29743513-5 2018 SK1 synthesizes sphingosine-1-phosphate, which regulates autophagy in non-neuronal cells and in neurons. sphingosine 1-phosphate 16-39 sphingosine kinase 1 Homo sapiens 0-3 29743513-6 2018 We found that benzoxazine autophagy inducers upregulate SK1 and neuroprotective autophagy in neurons, but not in astrocytes. Benzoxazines 14-25 sphingosine kinase 1 Homo sapiens 56-59 29643855-3 2018 Since increased generation of the lipid mediator sphingosine-1-phosphate (S1P) by sphingosine kinase (SPHK) has been linked to oncogenesis, we studied the involvement of the two SPHK isoforms (SPHK1 and SPHK2) in the regulation of neoplastic human MC growth. sphingosine 1-phosphate 49-72 sphingosine kinase 1 Homo sapiens 82-100 29643855-3 2018 Since increased generation of the lipid mediator sphingosine-1-phosphate (S1P) by sphingosine kinase (SPHK) has been linked to oncogenesis, we studied the involvement of the two SPHK isoforms (SPHK1 and SPHK2) in the regulation of neoplastic human MC growth. sphingosine 1-phosphate 49-72 sphingosine kinase 1 Homo sapiens 102-106 29643855-6 2018 Combination treatment of SPHK inhibitors with KIT inhibitors showed greater growth inhibition of D816V-KIT MCs than either inhibitor alone. d816v 97-102 sphingosine kinase 1 Homo sapiens 25-29 29484372-5 2018 We concluded: i) initial SphK inhibitors based on sphingosine have low specificity with several important off-targets. Sphingosine 50-61 sphingosine kinase 1 Homo sapiens 25-29 29695761-8 2018 (ii) The detection of outliers using variance analysis related to private epimutations identified a dozen CpGs in converters only and highlighted two genes (RAC1 and SPHK1) from the sphingolipid signaling pathway. Sphingolipids 182-194 sphingosine kinase 1 Homo sapiens 166-171 29605826-12 2018 The expression of SphK1 was positively correlated with poor overall survival (OS) and progression-free survival (PFS), as well as poor response to 5-FU and doxorubicin. Fluorouracil 147-151 sphingosine kinase 1 Homo sapiens 18-23 29605826-12 2018 The expression of SphK1 was positively correlated with poor overall survival (OS) and progression-free survival (PFS), as well as poor response to 5-FU and doxorubicin. Doxorubicin 156-167 sphingosine kinase 1 Homo sapiens 18-23 29605826-14 2018 Furthermore, silencing of SphK1 by Ad-SPHK1-siRNA or SphK1 inhibitor PF543 sensitized TNBCs to 5-FU and doxorubicin. tnbcs 86-91 sphingosine kinase 1 Homo sapiens 26-31 29605826-14 2018 Furthermore, silencing of SphK1 by Ad-SPHK1-siRNA or SphK1 inhibitor PF543 sensitized TNBCs to 5-FU and doxorubicin. tnbcs 86-91 sphingosine kinase 1 Homo sapiens 38-43 29605826-14 2018 Furthermore, silencing of SphK1 by Ad-SPHK1-siRNA or SphK1 inhibitor PF543 sensitized TNBCs to 5-FU and doxorubicin. tnbcs 86-91 sphingosine kinase 1 Homo sapiens 53-58 29605826-14 2018 Furthermore, silencing of SphK1 by Ad-SPHK1-siRNA or SphK1 inhibitor PF543 sensitized TNBCs to 5-FU and doxorubicin. Fluorouracil 95-99 sphingosine kinase 1 Homo sapiens 26-31 29605826-14 2018 Furthermore, silencing of SphK1 by Ad-SPHK1-siRNA or SphK1 inhibitor PF543 sensitized TNBCs to 5-FU and doxorubicin. Fluorouracil 95-99 sphingosine kinase 1 Homo sapiens 53-58 29605826-14 2018 Furthermore, silencing of SphK1 by Ad-SPHK1-siRNA or SphK1 inhibitor PF543 sensitized TNBCs to 5-FU and doxorubicin. Doxorubicin 104-115 sphingosine kinase 1 Homo sapiens 26-31 29167124-10 2018 These data reveal the importance of miR-1 in regulating SphK1 expression during hypoxia in PASMCs. pasmcs 91-97 sphingosine kinase 1 Homo sapiens 56-61 29260413-0 2018 Berberine protects HK-2 cells from hypoxia/reoxygenation induced apoptosis via inhibiting SPHK1 expression. Berberine 0-9 sphingosine kinase 1 Homo sapiens 90-95 29326159-1 2018 Sphingosine kinase 1 (SK1) is required for production of sphingosine-1-phosphate (S1P) and thereby regulates many cellular processes, including cellular growth, immune cell trafficking, and inflammation. sphingosine 1-phosphate 57-80 sphingosine kinase 1 Homo sapiens 0-20 29326159-1 2018 Sphingosine kinase 1 (SK1) is required for production of sphingosine-1-phosphate (S1P) and thereby regulates many cellular processes, including cellular growth, immune cell trafficking, and inflammation. sphingosine 1-phosphate 57-80 sphingosine kinase 1 Homo sapiens 22-25 29326159-1 2018 Sphingosine kinase 1 (SK1) is required for production of sphingosine-1-phosphate (S1P) and thereby regulates many cellular processes, including cellular growth, immune cell trafficking, and inflammation. sphingosine 1-phosphate 82-85 sphingosine kinase 1 Homo sapiens 0-20 29326159-1 2018 Sphingosine kinase 1 (SK1) is required for production of sphingosine-1-phosphate (S1P) and thereby regulates many cellular processes, including cellular growth, immune cell trafficking, and inflammation. sphingosine 1-phosphate 82-85 sphingosine kinase 1 Homo sapiens 22-25 29326159-2 2018 To produce S1P, SK1 must access sphingosine directly from membranes. Sphingosine 32-43 sphingosine kinase 1 Homo sapiens 16-19 29326159-4 2018 We used hydrogen/deuterium exchange MS to study interactions of SK1 with membrane vesicles. Hydrogen 8-16 sphingosine kinase 1 Homo sapiens 64-67 29326159-4 2018 We used hydrogen/deuterium exchange MS to study interactions of SK1 with membrane vesicles. Deuterium 17-26 sphingosine kinase 1 Homo sapiens 64-67 29326159-9 2018 Furthermore, we demonstrated that SK1 uses a single contiguous interface, consisting of an electrostatic site and a hydrophobic site, to interact with membrane-associated anionic phospholipids. Phospholipids 179-192 sphingosine kinase 1 Homo sapiens 34-37 29260413-5 2018 Here, we found increased SPHK1 levels in H/R injured HK-2 cells, which could be significantly down regulated after berberine treatment. Berberine 115-124 sphingosine kinase 1 Homo sapiens 25-30 29260413-7 2018 So, in our present study, we planned to investigate whether SPHK1 participated in the anti-apoptosis process of berberine in H/R injured HK-2 cells. Berberine 112-121 sphingosine kinase 1 Homo sapiens 60-65 29260413-8 2018 Our study confirmed the protective effect of berberine against H/R-induced apoptosis in HK-2 cells through promoting cells viability, inhibiting cells apoptosis, and down-regulating p-P38, caspase-3, caspase-9 as well as SPHK1, while up regulating the ratio of Bcl-2/Bax. Berberine 45-54 sphingosine kinase 1 Homo sapiens 221-226 29260413-9 2018 However, SPHK1 overexpression in HK-2 cells induced severe apoptosis, which can be significantly ameliorated with additional berberine treatment. Berberine 125-134 sphingosine kinase 1 Homo sapiens 9-14 29260413-10 2018 We concluded that berberine could remarkably prevent H/R-induced apoptosis in HK-2 cells through down-regulating SPHK1 expression levels, and the mechanisms included the suppression of p38 MAPK activation and mitochondrial stress pathways. Berberine 18-27 sphingosine kinase 1 Homo sapiens 113-118 29260413-10 2018 We concluded that berberine could remarkably prevent H/R-induced apoptosis in HK-2 cells through down-regulating SPHK1 expression levels, and the mechanisms included the suppression of p38 MAPK activation and mitochondrial stress pathways. r 55-56 sphingosine kinase 1 Homo sapiens 113-118 27829348-9 2018 MD simulation of ligand 5 showed that the ligand-SK1 complex reached equilibrium with favorable hydrogen bonding and hydrophobic interactions. Hydrogen 96-104 sphingosine kinase 1 Homo sapiens 49-52 29643998-2 2018 Sphingosine kinase 1 (SphK1), a key regulator of sphingolipid metabolites, is over-expressed in human hepatocellular carcinoma (HCC) and our previous studies have shown that SphK1 is important in liver injury. Sphingolipids 49-61 sphingosine kinase 1 Homo sapiens 0-20 29643998-2 2018 Sphingosine kinase 1 (SphK1), a key regulator of sphingolipid metabolites, is over-expressed in human hepatocellular carcinoma (HCC) and our previous studies have shown that SphK1 is important in liver injury. Sphingolipids 49-61 sphingosine kinase 1 Homo sapiens 22-27 29643998-2 2018 Sphingosine kinase 1 (SphK1), a key regulator of sphingolipid metabolites, is over-expressed in human hepatocellular carcinoma (HCC) and our previous studies have shown that SphK1 is important in liver injury. Sphingolipids 49-61 sphingosine kinase 1 Homo sapiens 174-179 29385066-5 2018 Estrogen activates sphingosine kinase 1 (SphK1) and amplifies the intracellular concentration of sphingosine-1-phosphate (S1P) in breast cancer cells during stimulation of proliferation and survival. sphingosine 1-phosphate 97-120 sphingosine kinase 1 Homo sapiens 41-46 29385066-11 2018 Considering that sphingolipid signaling has a prominent role in terminally differentiated cells, the impact should be considered when designing specific SphK/S1PR inhibitors. Sphingolipids 17-29 sphingosine kinase 1 Homo sapiens 153-157 29360739-8 2018 Moreover, peretinoin significantly inhibited the expression of SPHK1, a potential inhibitor of HDAC activity, and might be involved in hepatic inflammation, fibrosis, and HCC. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 10-20 sphingosine kinase 1 Homo sapiens 63-68 29360739-9 2018 SPHK1 overexpression in cells cancelled the inhibition of HBV replication induced by peretinoin. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 85-95 sphingosine kinase 1 Homo sapiens 0-5 28968647-2 2018 Sphingosine kinase 1 (SphK1) is a key metabolic enzyme for the formation of pro-survival S1P from pro-apoptotic ceramide. Ceramides 112-120 sphingosine kinase 1 Homo sapiens 0-20 28968647-2 2018 Sphingosine kinase 1 (SphK1) is a key metabolic enzyme for the formation of pro-survival S1P from pro-apoptotic ceramide. Ceramides 112-120 sphingosine kinase 1 Homo sapiens 22-27 30060814-1 2018 Sphingosine kinases (SK1 and SK2) are key, druggable targets within the sphingolipid metabolism pathway that promote tumor growth and pathologic inflammation. Sphingolipids 72-84 sphingosine kinase 1 Homo sapiens 21-24 29368980-1 2018 The bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) and the enzyme that produces it, SPHK1 (sphingosine kinase 1), regulate many processes important for the etiology of cancer. Sphingolipids 14-26 sphingosine kinase 1 Homo sapiens 101-106 29368980-1 2018 The bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) and the enzyme that produces it, SPHK1 (sphingosine kinase 1), regulate many processes important for the etiology of cancer. Sphingolipids 14-26 sphingosine kinase 1 Homo sapiens 108-128 29368980-1 2018 The bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) and the enzyme that produces it, SPHK1 (sphingosine kinase 1), regulate many processes important for the etiology of cancer. sphingosine 1-phosphate 38-61 sphingosine kinase 1 Homo sapiens 101-106 29368980-1 2018 The bioactive sphingolipid metabolite sphingosine-1-phosphate (S1P) and the enzyme that produces it, SPHK1 (sphingosine kinase 1), regulate many processes important for the etiology of cancer. sphingosine 1-phosphate 38-61 sphingosine kinase 1 Homo sapiens 108-128 29368980-5 2018 SK1-I, an analog of sphingosine and isozyme-specific SPHK1 inhibitor, suppressed cancer cell growth and clonogenic survival in a TP53-dependent manner. Sphingosine 20-31 sphingosine kinase 1 Homo sapiens 0-3 30453304-1 2018 BACKGROUND/AIMS: The underlying molecular mechanisms involved in sphingosine kinase 1 (SphK1)/sphingosine 1-phosphate (S1P) mediation of platelet-derived growth factor (PDGF)-induced pulmonary arterial smooth muscle cell (PASMC) proliferation are still unclear, and the present study aims to address this issue. sphingosine 1-phosphate 94-117 sphingosine kinase 1 Homo sapiens 87-92 30453304-6 2018 Pre-silencing of SphK1 with siRNA reversed PDGF-induced S1P production, miR-21 up-regulation, BMPRII and Id1 down-regulation, as well as PASMC proliferation. pasmc 137-142 sphingosine kinase 1 Homo sapiens 17-22 30453304-10 2018 CONCLUSION: Our study indicates that SphK1/S1P pathway plays an important role in PDGF-induced PASMC proliferation via miR-21/BMPRII/Id1 axis and targeting against SphK1/S1P axis might be a novel strategy in the prevention and treatment of pulmonary arterial hypertension (PAH). pasmc 95-100 sphingosine kinase 1 Homo sapiens 37-42 28560513-1 2018 Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its production, sphingosine kinase-1 (SphK-1), are dysregulated in multiple human diseases including cancer, multiple sclerosis (MS), diabetes, neurological diseases, fibrosis, and certain pathologies associated with impaired angiogenesis such as age-related macular degeneration (AMD). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 87-107 29167409-4 2018 During phosphate-induced VSMC mineralization, endogenous S1P levels increased accompanied by increased sphingosine kinase (SK) activity and increased mRNA expression of SK1 and SK2. Phosphates 7-16 sphingosine kinase 1 Homo sapiens 169-172 28050792-12 2018 The inhibition of Sphk1 and Akt by eASN may lead to disturbances of survival pathways. easn 35-39 sphingosine kinase 1 Homo sapiens 18-23 29147025-5 2018 Moreover, the metabolism of ceramide for S1P biosynthesis, which is mediated by sphingosine kinase 1 and 2, and its role in influencing cancer cell growth, drug resistance and tumour metastasis through S1PR-dependent or receptor-independent signalling are highlighted. Ceramides 28-36 sphingosine kinase 1 Homo sapiens 80-106 28560513-1 2018 Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its production, sphingosine kinase-1 (SphK-1), are dysregulated in multiple human diseases including cancer, multiple sclerosis (MS), diabetes, neurological diseases, fibrosis, and certain pathologies associated with impaired angiogenesis such as age-related macular degeneration (AMD). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 109-115 29208982-0 2017 Peretinoin, an acyclic retinoid, inhibits hepatocarcinogenesis by suppressing sphingosine kinase 1 expression in vitro and in vivo. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 0-10 sphingosine kinase 1 Homo sapiens 78-98 29207959-8 2017 RESULTS: High IGF1R and SphK1 protein co-expression in tumor tissue was associated with improved OS specifically in ER-positive disease and stratified for anti-endocrine therapy. Osmium 97-99 sphingosine kinase 1 Homo sapiens 24-29 29208982-2 2017 Because sphingosine kinase 1 and sphingosine kinase 2 (SPHK 1/2) are both essential for sphingosine-1-phospate production, they could be a therapeutic target in various cancers. sphingosine-1-phospate 88-110 sphingosine kinase 1 Homo sapiens 8-28 29208982-2 2017 Because sphingosine kinase 1 and sphingosine kinase 2 (SPHK 1/2) are both essential for sphingosine-1-phospate production, they could be a therapeutic target in various cancers. sphingosine-1-phospate 88-110 sphingosine kinase 1 Homo sapiens 55-63 29208982-4 2017 In this study, we assessed effects of peretinoin on SPHK expression and liver cancer development in vitro and in vivo. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 38-48 sphingosine kinase 1 Homo sapiens 52-56 29208982-5 2017 We examined effects of peretinoin on expression, enzymatic and promoter activity of SPHK1 in a human hepatoma cell line, Huh-7. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 23-33 sphingosine kinase 1 Homo sapiens 84-89 29208982-7 2017 Peretinoin treatment of Huh-7 cells reduced mRNA levels, protein expression and enzymatic activity of SPHK1. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 0-10 sphingosine kinase 1 Homo sapiens 102-107 29208982-8 2017 Peretinoin reduced SPHK1 promoter activity; this effect of peretinoin was blocked by overexpression of Sp1, a transcription factor. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 0-10 sphingosine kinase 1 Homo sapiens 19-24 29208982-8 2017 Peretinoin reduced SPHK1 promoter activity; this effect of peretinoin was blocked by overexpression of Sp1, a transcription factor. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 59-69 sphingosine kinase 1 Homo sapiens 19-24 29208982-9 2017 Deletion of all Sp1 binding sites within the SPHK1 promoter region abolished SPHK1 promoter activity, suggesting that peretinoin reduced mRNA levels of SPHK1 via Sp1. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 118-128 sphingosine kinase 1 Homo sapiens 45-50 29208982-9 2017 Deletion of all Sp1 binding sites within the SPHK1 promoter region abolished SPHK1 promoter activity, suggesting that peretinoin reduced mRNA levels of SPHK1 via Sp1. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 118-128 sphingosine kinase 1 Homo sapiens 77-82 29208982-9 2017 Deletion of all Sp1 binding sites within the SPHK1 promoter region abolished SPHK1 promoter activity, suggesting that peretinoin reduced mRNA levels of SPHK1 via Sp1. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 118-128 sphingosine kinase 1 Homo sapiens 77-82 29208982-11 2017 Our data showed crucial roles of SPHK1 in hepatocarcinogenesis and suggests that peretinoin prevents hepatocarcinogenesis by suppressing mRNA levels of SPHK1. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 81-91 sphingosine kinase 1 Homo sapiens 33-38 29208982-11 2017 Our data showed crucial roles of SPHK1 in hepatocarcinogenesis and suggests that peretinoin prevents hepatocarcinogenesis by suppressing mRNA levels of SPHK1. (2E,4E,6E,10E)-3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid 81-91 sphingosine kinase 1 Homo sapiens 152-157 28842426-4 2017 Perturbing the sphingolipid pathway by disrupting Sphk genes during pregnancy led to excessive NET formation exclusively at the fetomaternal interface and early fetal death. Sphingolipids 15-27 sphingosine kinase 1 Homo sapiens 50-54 29019499-6 2017 The ss-DSSC devices with these self-assembled ordered ionic crystals with a carbazole based sensitizer (SK1) achieved a power conversion efficiency (PCE) of 4.2% and 5.2% for al and ar arrangement, respectively. carbazole 76-85 sphingosine kinase 1 Homo sapiens 104-107 29078883-7 2017 Expression of sphingosine kinase 1, the enzyme that produces S1P and links inflammation and cancer, tended to be higher in doxorubicin-resistant human cancer and cell lines. Doxorubicin 123-134 sphingosine kinase 1 Homo sapiens 14-34 29156544-9 2017 A weak positive correlation was found between high-density lipoprotein (HDL) cholesterol and SphK1, and HDL and ceramide before treatment (p = 0.036, r = 0.3826; p = 0.018, r = 0.4286, respectively. Cholesterol 77-88 sphingosine kinase 1 Homo sapiens 93-98 29156544-10 2017 Conclusions:C. membranaceus uses the ceramide pathway by modulating the SphK1/SphK2 ratio and increasing SPL to generate oxidative stress and consequently apoptosis. Ceramides 37-45 sphingosine kinase 1 Homo sapiens 72-77 29113071-0 2017 Development of 1,2,3-Triazole-Based Sphingosine Kinase Inhibitors and Their Evaluation as Antiproliferative Agents. Triazoles 15-29 sphingosine kinase 1 Homo sapiens 36-54 29113071-2 2017 All the compounds, containing a lipophilic tail and a polar headgroup, were evaluated as sphingosine kinase (SphK) inhibitors by assessing their ability to interfere with the acetylcholine (Ach) induced relaxation of aortic rings pre-contracted with phenylephrine. Acetylcholine 190-193 sphingosine kinase 1 Homo sapiens 109-113 29113071-4 2017 Compounds 5h and 8f, identified as the most efficient antiproliferative agents, showed a different selectivity profile, with 8f being selective for SphK1. 5h 10-12 sphingosine kinase 1 Homo sapiens 148-153 29113071-4 2017 Compounds 5h and 8f, identified as the most efficient antiproliferative agents, showed a different selectivity profile, with 8f being selective for SphK1. 8f 17-19 sphingosine kinase 1 Homo sapiens 148-153 29019499-6 2017 The ss-DSSC devices with these self-assembled ordered ionic crystals with a carbazole based sensitizer (SK1) achieved a power conversion efficiency (PCE) of 4.2% and 5.2% for al and ar arrangement, respectively. Aluminum 65-67 sphingosine kinase 1 Homo sapiens 104-107 29019499-6 2017 The ss-DSSC devices with these self-assembled ordered ionic crystals with a carbazole based sensitizer (SK1) achieved a power conversion efficiency (PCE) of 4.2% and 5.2% for al and ar arrangement, respectively. Argon 77-79 sphingosine kinase 1 Homo sapiens 104-107 28822281-1 2017 Sphingosine kinase 1 (SphK1), the enzyme that produces the bioactive sphingolipid metabolite, sphingosine-1-phosphate, is a promising new molecular target for therapeutic intervention in cancer and inflammatory diseases. Sphingolipids 69-81 sphingosine kinase 1 Homo sapiens 0-20 29085869-8 2017 These findings, in conjunction with fluid-flow induced upregulation of Sphk1, the kinase responsible for phosphorylation of sphingosine, suggest that mechanical stimulation of osteocytes leads to an increase in intracellular S1P. Sphingosine 124-135 sphingosine kinase 1 Homo sapiens 71-76 28822281-1 2017 Sphingosine kinase 1 (SphK1), the enzyme that produces the bioactive sphingolipid metabolite, sphingosine-1-phosphate, is a promising new molecular target for therapeutic intervention in cancer and inflammatory diseases. Sphingolipids 69-81 sphingosine kinase 1 Homo sapiens 22-27 28822281-1 2017 Sphingosine kinase 1 (SphK1), the enzyme that produces the bioactive sphingolipid metabolite, sphingosine-1-phosphate, is a promising new molecular target for therapeutic intervention in cancer and inflammatory diseases. sphingosine 1-phosphate 94-117 sphingosine kinase 1 Homo sapiens 0-20 28822281-1 2017 Sphingosine kinase 1 (SphK1), the enzyme that produces the bioactive sphingolipid metabolite, sphingosine-1-phosphate, is a promising new molecular target for therapeutic intervention in cancer and inflammatory diseases. sphingosine 1-phosphate 94-117 sphingosine kinase 1 Homo sapiens 22-27 28684192-5 2017 Sphingosine 1-phosphate (S1P), a lipid generated after sphingosine phosphorylation by sphingosine kinases 1 and 2 (SK1/2), plays important roles in cell proliferation, differentiation and survival. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 86-113 28991193-1 2017 Sphingosine kinase 1 (Sphk1) is a highly conserved lipid kinase that phosphorylates sphingosine to form sphingosine-1-phosphate (S1P). Sphingosine 84-95 sphingosine kinase 1 Homo sapiens 0-20 28991193-1 2017 Sphingosine kinase 1 (Sphk1) is a highly conserved lipid kinase that phosphorylates sphingosine to form sphingosine-1-phosphate (S1P). Sphingosine 84-95 sphingosine kinase 1 Homo sapiens 22-27 28991193-1 2017 Sphingosine kinase 1 (Sphk1) is a highly conserved lipid kinase that phosphorylates sphingosine to form sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 104-127 sphingosine kinase 1 Homo sapiens 0-20 28991193-1 2017 Sphingosine kinase 1 (Sphk1) is a highly conserved lipid kinase that phosphorylates sphingosine to form sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 104-127 sphingosine kinase 1 Homo sapiens 22-27 28775072-2 2017 Sphk (sphingosine kinase)1 phosphorylates sphingosine to S1P (sphingosine-1-phosphate). Sphingosine 6-17 sphingosine kinase 1 Homo sapiens 0-4 28775072-2 2017 Sphk (sphingosine kinase)1 phosphorylates sphingosine to S1P (sphingosine-1-phosphate). sphingosine 1-phosphate 57-60 sphingosine kinase 1 Homo sapiens 0-4 28775072-2 2017 Sphk (sphingosine kinase)1 phosphorylates sphingosine to S1P (sphingosine-1-phosphate). sphingosine 1-phosphate 57-60 sphingosine kinase 1 Homo sapiens 6-24 28775072-2 2017 Sphk (sphingosine kinase)1 phosphorylates sphingosine to S1P (sphingosine-1-phosphate). sphingosine 1-phosphate 62-85 sphingosine kinase 1 Homo sapiens 0-4 28775072-2 2017 Sphk (sphingosine kinase)1 phosphorylates sphingosine to S1P (sphingosine-1-phosphate). sphingosine 1-phosphate 62-85 sphingosine kinase 1 Homo sapiens 6-24 28684192-12 2017 Using the pharmacological drugs SKi (SK pan-inhibitor), PF-543 (SK1 specific inhibitor) or K-145 (SK2 specific inhibitor), we showed that the inhibition of SK1 and/or SK2 decreased matrix mineralization, alkaline phosphatase activity and the mRNA expression of Runx2 and Bglap in chondrocytes and osteoblasts. PF-543 56-62 sphingosine kinase 1 Homo sapiens 156-159 28684192-5 2017 Sphingosine 1-phosphate (S1P), a lipid generated after sphingosine phosphorylation by sphingosine kinases 1 and 2 (SK1/2), plays important roles in cell proliferation, differentiation and survival. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 115-120 28684192-12 2017 Using the pharmacological drugs SKi (SK pan-inhibitor), PF-543 (SK1 specific inhibitor) or K-145 (SK2 specific inhibitor), we showed that the inhibition of SK1 and/or SK2 decreased matrix mineralization, alkaline phosphatase activity and the mRNA expression of Runx2 and Bglap in chondrocytes and osteoblasts. k-145 91-96 sphingosine kinase 1 Homo sapiens 156-159 28684192-5 2017 Sphingosine 1-phosphate (S1P), a lipid generated after sphingosine phosphorylation by sphingosine kinases 1 and 2 (SK1/2), plays important roles in cell proliferation, differentiation and survival. Sphingosine 55-66 sphingosine kinase 1 Homo sapiens 86-113 28684192-5 2017 Sphingosine 1-phosphate (S1P), a lipid generated after sphingosine phosphorylation by sphingosine kinases 1 and 2 (SK1/2), plays important roles in cell proliferation, differentiation and survival. Sphingosine 55-66 sphingosine kinase 1 Homo sapiens 115-120 28684192-12 2017 Using the pharmacological drugs SKi (SK pan-inhibitor), PF-543 (SK1 specific inhibitor) or K-145 (SK2 specific inhibitor), we showed that the inhibition of SK1 and/or SK2 decreased matrix mineralization, alkaline phosphatase activity and the mRNA expression of Runx2 and Bglap in chondrocytes and osteoblasts. PF-543 56-62 sphingosine kinase 1 Homo sapiens 64-67 28695300-2 2017 Sphingosine-1-phosphate (S1P) receptor antagonist and sphingosine kinase 1 (SK1) inhibitor FTY720 (FTY) has promising anticancer properties, however, it causes systemic lymphopenia which impairs its use in cancer patients. Fingolimod Hydrochloride 91-97 sphingosine kinase 1 Homo sapiens 76-79 28695300-2 2017 Sphingosine-1-phosphate (S1P) receptor antagonist and sphingosine kinase 1 (SK1) inhibitor FTY720 (FTY) has promising anticancer properties, however, it causes systemic lymphopenia which impairs its use in cancer patients. Fingolimod Hydrochloride 91-97 sphingosine kinase 1 Homo sapiens 54-74 28982849-4 2017 MATERIALS AND METHODS: Sphingosine kinase 1 expression was evaluated using immunohistochemical staining in 110 formalin-fixed, paraffin-embedded papillary thyroid carcinoma tissue samples. Formaldehyde 111-119 sphingosine kinase 1 Homo sapiens 23-43 28982849-4 2017 MATERIALS AND METHODS: Sphingosine kinase 1 expression was evaluated using immunohistochemical staining in 110 formalin-fixed, paraffin-embedded papillary thyroid carcinoma tissue samples. Paraffin 127-135 sphingosine kinase 1 Homo sapiens 23-43 28672103-3 2017 Sphingosine 1-phosphate (S1P), a bioactive sphingolipid, is produced intracellularly by sphingosine kinase (SphK) and is regarded as a second signaling molecule that regulates inflammation, proliferation, angiogenesis, and metastasis. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 88-106 28618839-2 2017 Subsequent studies later identified two related kinases, sphingosine kinase 1 and 2, which are responsible for the phosphorylation of sphingosine to S1P. sphingosine 1-phosphate 149-152 sphingosine kinase 1 Homo sapiens 57-83 28672103-3 2017 Sphingosine 1-phosphate (S1P), a bioactive sphingolipid, is produced intracellularly by sphingosine kinase (SphK) and is regarded as a second signaling molecule that regulates inflammation, proliferation, angiogenesis, and metastasis. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 88-106 28672103-3 2017 Sphingosine 1-phosphate (S1P), a bioactive sphingolipid, is produced intracellularly by sphingosine kinase (SphK) and is regarded as a second signaling molecule that regulates inflammation, proliferation, angiogenesis, and metastasis. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 108-112 28672103-3 2017 Sphingosine 1-phosphate (S1P), a bioactive sphingolipid, is produced intracellularly by sphingosine kinase (SphK) and is regarded as a second signaling molecule that regulates inflammation, proliferation, angiogenesis, and metastasis. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 108-112 28876923-3 2017 A lysine derivative of Sph, SphK, was site-selectively incorporated into the extracellular loop regions (ECLs) of GCGR and GLP-1R, two members of class B G protein-coupled receptors (GPCRs) in mammalian cells with the incorporation efficiency dependent on the location. Lysine 2-8 sphingosine kinase 1 Homo sapiens 28-32 29169412-9 2017 Cer content in MDA-MB231 cells was reduced by TNF-alpha, which was prevented by the pretreatment of SPHK1 inhibitor DMS. dms 116-119 sphingosine kinase 1 Homo sapiens 100-105 28420720-1 2017 Purpose: Sphingosine kinases (SK1 and SK2) regulate tumor growth by generating the mitogenic and proinflammatory lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 119-142 sphingosine kinase 1 Homo sapiens 30-33 28869494-5 2017 Here we discuss additional theories of SphK cellular mislocation and aberrant "dicing and splicing" as contributors to cancer cell biology and as key determinants of the success or failure of SphK/S1P (sphingosine 1 phosphate) based therapeutics. sphingosine 1-phosphate 202-225 sphingosine kinase 1 Homo sapiens 39-43 28729416-1 2017 Sphingosine kinase 1 (SK1) is a key regulator of the cellular balance between proapoptotic and prosurvival sphingolipids. Sphingolipids 107-120 sphingosine kinase 1 Homo sapiens 0-20 28729416-1 2017 Sphingosine kinase 1 (SK1) is a key regulator of the cellular balance between proapoptotic and prosurvival sphingolipids. Sphingolipids 107-120 sphingosine kinase 1 Homo sapiens 22-25 28789830-3 2017 Therefore, SphK1 might be a novel target for treatment of PAH. p-Aminohippuric Acid 58-61 sphingosine kinase 1 Homo sapiens 11-16 28789830-4 2017 However, in our opinion, more refined strategies to target SphK1 are needed because this enzyme is protective against endothelial dysfunction and can become resistant to SphK1 inhibitors in vascular smooth muscle, thereby potentially limiting their effectiveness in PAH. p-Aminohippuric Acid 266-269 sphingosine kinase 1 Homo sapiens 59-64 28878674-1 2017 The sphingosine kinases 1 and 2 (SphK1 and 2) catalyze the phosphorylation of the lipid, sphingosine, generating the signal transmitter, sphingosine 1-phosphate (S1P). Sphingosine 4-15 sphingosine kinase 1 Homo sapiens 33-44 28878674-1 2017 The sphingosine kinases 1 and 2 (SphK1 and 2) catalyze the phosphorylation of the lipid, sphingosine, generating the signal transmitter, sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 137-160 sphingosine kinase 1 Homo sapiens 4-31 28878674-1 2017 The sphingosine kinases 1 and 2 (SphK1 and 2) catalyze the phosphorylation of the lipid, sphingosine, generating the signal transmitter, sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 137-160 sphingosine kinase 1 Homo sapiens 33-44 28420720-1 2017 Purpose: Sphingosine kinases (SK1 and SK2) regulate tumor growth by generating the mitogenic and proinflammatory lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 144-147 sphingosine kinase 1 Homo sapiens 30-33 28408190-5 2017 Based on this study, we identified two isoform selective inhibitors, including the m-phenylene derivative 4 [IC50 (SphK1) >=30muM; IC50 (SphK2)=2.2muM] and the methyl ether derivative 22 [IC50 (SphK1)=4.0muM; IC50 (SphK2) >=30muM]. m-phenylene 83-94 sphingosine kinase 1 Homo sapiens 115-120 28744400-7 2017 The overexpression of Sphk1 could significantly abrogate the anti-growth and anti-metastasis effects of hispidulin, whereas the siRNA-targeting Sphk1 or Sphk1 inhibitor was able to augment the anticancer effects of hispidulin against RCC. hispidulin 104-114 sphingosine kinase 1 Homo sapiens 22-27 29531546-2 2017 Sphingosine kinase 1 (SPHK1) and its product sphingosine-1-phosphate (S1P) are the essential key regulator molecules in breast cancer through their ability to promote cell proliferation, angiogenesis, cell proliferation, and lymphagiogenesis. sphingosine 1-phosphate 45-68 sphingosine kinase 1 Homo sapiens 0-20 29531546-2 2017 Sphingosine kinase 1 (SPHK1) and its product sphingosine-1-phosphate (S1P) are the essential key regulator molecules in breast cancer through their ability to promote cell proliferation, angiogenesis, cell proliferation, and lymphagiogenesis. sphingosine 1-phosphate 45-68 sphingosine kinase 1 Homo sapiens 22-27 28433634-4 2017 In the present study, we have investigated for the first time the anticancer efficacy and underlying molecular mechanisms of combined administration of 5-FU and dual Sphk1/Sphk2 inhibitor SKI-II (4-[[4-(4-chlorophenyl)-1,3-thiazol-2-yl]amino]phenol) in HepG2 hepatocellular carcinoma cells. Shikimic Acid 188-191 sphingosine kinase 1 Homo sapiens 166-171 28433634-4 2017 In the present study, we have investigated for the first time the anticancer efficacy and underlying molecular mechanisms of combined administration of 5-FU and dual Sphk1/Sphk2 inhibitor SKI-II (4-[[4-(4-chlorophenyl)-1,3-thiazol-2-yl]amino]phenol) in HepG2 hepatocellular carcinoma cells. N'-((2-hydroxy-1-naphthyl)methylene)-3-(2-naphthyl)-1H-pyrazole-5-carbohydrazide 196-248 sphingosine kinase 1 Homo sapiens 166-171 28433634-6 2017 Additional Western blot analyses have shown that possible mechanisms underlying enhanced sensitivity to 5-FU induced by dual Sphk 1/2 inhibition could include abrogation of FAK-regulated IGF-1R activity and down-regulation of osteopontin expression culminating in the inhibition of NF-kappaB activity and its downstream signalling mediated by sirtuin 1 and p38 MAPK. Fluorouracil 104-108 sphingosine kinase 1 Homo sapiens 125-131 28587987-1 2017 The sphingosine kinase 1 (SK1)/sphingosine-1-phosphate (S1P) signaling pathway is a crucial target for numerous human diseases from cancer to cardiovascular diseases. sphingosine 1-phosphate 31-54 sphingosine kinase 1 Homo sapiens 4-24 28587987-1 2017 The sphingosine kinase 1 (SK1)/sphingosine-1-phosphate (S1P) signaling pathway is a crucial target for numerous human diseases from cancer to cardiovascular diseases. sphingosine 1-phosphate 31-54 sphingosine kinase 1 Homo sapiens 26-29 27220679-0 2017 SphK1 inhibitor potentiates the anti-cancer effect of EGCG on leukaemia cells. epigallocatechin gallate 54-58 sphingosine kinase 1 Homo sapiens 0-5 28615679-2 2017 We have recently discovered a new molecular mechanism of prostate cancer docetaxel chemoresistance mediated by the mammalian target of rapamycin (mTOR)/sphingosine-kinase-1 (SK1) pathway. Docetaxel 73-82 sphingosine kinase 1 Homo sapiens 152-172 28615679-2 2017 We have recently discovered a new molecular mechanism of prostate cancer docetaxel chemoresistance mediated by the mammalian target of rapamycin (mTOR)/sphingosine-kinase-1 (SK1) pathway. Docetaxel 73-82 sphingosine kinase 1 Homo sapiens 174-177 28615679-5 2017 In hormone-insensitive prostate (PC3) and breast (MDA-MB-231 and BT-549) cancer cell lines the mTOR inhibitor RAD001 (everolimus) has significantly inhibited SK1 and VEGF expression, while low dose (5 nM) docetaxel had no significant effect. Everolimus 118-128 sphingosine kinase 1 Homo sapiens 158-161 28433634-7 2017 Our results clearly show that pharmacological blockade of both Sphk isoforms represents a promising strategy to boost the anti-tumour efficacy of 5-FU and provide a rationale for further in vivo studies into the possible use of SKI-II inhibitor as an adjunct to 5-FU treatment in HCC. Fluorouracil 146-150 sphingosine kinase 1 Homo sapiens 63-67 28433634-7 2017 Our results clearly show that pharmacological blockade of both Sphk isoforms represents a promising strategy to boost the anti-tumour efficacy of 5-FU and provide a rationale for further in vivo studies into the possible use of SKI-II inhibitor as an adjunct to 5-FU treatment in HCC. Fluorouracil 262-266 sphingosine kinase 1 Homo sapiens 63-67 28408190-5 2017 Based on this study, we identified two isoform selective inhibitors, including the m-phenylene derivative 4 [IC50 (SphK1) >=30muM; IC50 (SphK2)=2.2muM] and the methyl ether derivative 22 [IC50 (SphK1)=4.0muM; IC50 (SphK2) >=30muM]. m-phenylene 83-94 sphingosine kinase 1 Homo sapiens 197-202 28408190-5 2017 Based on this study, we identified two isoform selective inhibitors, including the m-phenylene derivative 4 [IC50 (SphK1) >=30muM; IC50 (SphK2)=2.2muM] and the methyl ether derivative 22 [IC50 (SphK1)=4.0muM; IC50 (SphK2) >=30muM]. Ether 170-175 sphingosine kinase 1 Homo sapiens 115-120 28258651-4 2017 Indeed, expression of SK1 induced a high glycolytic rate, characterized by increased levels of lactate along with increased expression of the proton/monocarboxylate symporter MCT1, and decreased oxidative metabolism, associated with the accumulation of intermediates of the tricarboxylic acid cycle and reduction in CO2 production. Lactic Acid 95-102 sphingosine kinase 1 Homo sapiens 22-25 28415564-4 2017 SphK is expressed as two isozymes SphK1 and SphK2, transcribed from genes located on different chromosomes and both isozymes catalyze the phosphorylation of sphingosine to S1P. Sphingosine 157-168 sphingosine kinase 1 Homo sapiens 0-4 28415564-4 2017 SphK is expressed as two isozymes SphK1 and SphK2, transcribed from genes located on different chromosomes and both isozymes catalyze the phosphorylation of sphingosine to S1P. Sphingosine 157-168 sphingosine kinase 1 Homo sapiens 34-39 28406646-3 2017 In this article, we describe the design, synthesis, and biological evaluation of aminothiazole-based guanidine inhibitors of SphK. 2-aminothiazole 81-94 sphingosine kinase 1 Homo sapiens 125-129 28406646-3 2017 In this article, we describe the design, synthesis, and biological evaluation of aminothiazole-based guanidine inhibitors of SphK. Guanidine 101-110 sphingosine kinase 1 Homo sapiens 125-129 28406646-4 2017 Surprisingly, combining features of reported SphK1 inhibitors generated SphK1/2 dual inhibitor 20l (SLC4011540) (hSphK1 Ki = 120 nM, hSphK2 Ki = 90 nM) and SphK2 inhibitor 20dd (SLC4101431) (Ki = 90 nM, 100-fold SphK2 selectivity). 20dd 172-176 sphingosine kinase 1 Homo sapiens 45-50 28406646-4 2017 Surprisingly, combining features of reported SphK1 inhibitors generated SphK1/2 dual inhibitor 20l (SLC4011540) (hSphK1 Ki = 120 nM, hSphK2 Ki = 90 nM) and SphK2 inhibitor 20dd (SLC4101431) (Ki = 90 nM, 100-fold SphK2 selectivity). 20dd 172-176 sphingosine kinase 1 Homo sapiens 72-79 28174291-5 2017 Furthermore, we demonstrate that the main product of SK1, sphingosine-1-phosphate, controls the migration of intraislet endothelial cells in vitro. sphingosine 1-phosphate 58-81 sphingosine kinase 1 Homo sapiens 53-56 28502300-0 2017 [Down-regulation of sphingosine kinase 1 (SphK1) enhances the chemosensitivity to cisplatin in human colon cancer RKO cells]. Cisplatin 82-91 sphingosine kinase 1 Homo sapiens 20-40 28502300-0 2017 [Down-regulation of sphingosine kinase 1 (SphK1) enhances the chemosensitivity to cisplatin in human colon cancer RKO cells]. Cisplatin 82-91 sphingosine kinase 1 Homo sapiens 42-47 28502300-1 2017 Objective To investigate the effect of sphingosine kinase 1 (SphK1) gene silence on the sensitivity to cisplatin (DDP) in RKO colon cancer cell line and the potential mechanism. Cisplatin 103-112 sphingosine kinase 1 Homo sapiens 39-59 28502300-1 2017 Objective To investigate the effect of sphingosine kinase 1 (SphK1) gene silence on the sensitivity to cisplatin (DDP) in RKO colon cancer cell line and the potential mechanism. Cisplatin 103-112 sphingosine kinase 1 Homo sapiens 61-66 28502300-12 2017 Moreover, with the down-regulation of SphK1, the expressions of ki67 and Bcl-2 were depressed; the expressions of caspase-9 and caspase-3 were raised, especially after treated with DDP. Cisplatin 181-184 sphingosine kinase 1 Homo sapiens 38-43 28938571-9 2017 Further studies showed that CC223 inhibited sphingosine kinase 1 (SphK1) activity and induced reactive oxygen species (ROS) production in SKOV3 cells. CC-223 28-33 sphingosine kinase 1 Homo sapiens 44-64 28938571-9 2017 Further studies showed that CC223 inhibited sphingosine kinase 1 (SphK1) activity and induced reactive oxygen species (ROS) production in SKOV3 cells. CC-223 28-33 sphingosine kinase 1 Homo sapiens 66-71 28521610-1 2017 SPHK1 (sphingosine kinase 1), a regulator of sphingolipid metabolites, plays a causal role in the development of hepatocellular carcinoma (HCC) through augmenting HCC invasion and metastasis. Sphingolipids 45-57 sphingosine kinase 1 Homo sapiens 0-5 28521610-1 2017 SPHK1 (sphingosine kinase 1), a regulator of sphingolipid metabolites, plays a causal role in the development of hepatocellular carcinoma (HCC) through augmenting HCC invasion and metastasis. Sphingolipids 45-57 sphingosine kinase 1 Homo sapiens 7-27 28521610-7 2017 Moreover, the overexpression of SPHK1 produced intracellular sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 61-84 sphingosine kinase 1 Homo sapiens 32-37 28521610-7 2017 Moreover, the overexpression of SPHK1 produced intracellular sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 86-89 sphingosine kinase 1 Homo sapiens 32-37 28258651-4 2017 Indeed, expression of SK1 induced a high glycolytic rate, characterized by increased levels of lactate along with increased expression of the proton/monocarboxylate symporter MCT1, and decreased oxidative metabolism, associated with the accumulation of intermediates of the tricarboxylic acid cycle and reduction in CO2 production. Tricarboxylic Acids 274-292 sphingosine kinase 1 Homo sapiens 22-25 28258651-4 2017 Indeed, expression of SK1 induced a high glycolytic rate, characterized by increased levels of lactate along with increased expression of the proton/monocarboxylate symporter MCT1, and decreased oxidative metabolism, associated with the accumulation of intermediates of the tricarboxylic acid cycle and reduction in CO2 production. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 316-319 sphingosine kinase 1 Homo sapiens 22-25 28322796-6 2017 Inhibition of SPHK1 using siRNA or a pharmacological inhibitor decreased S1P levels in PC3-PR cells and inhibited proliferation in the presence or absence of PTX, suggesting that SPHK1 is at least partially responsible for PTX resistance. Paclitaxel 158-161 sphingosine kinase 1 Homo sapiens 14-19 28322796-6 2017 Inhibition of SPHK1 using siRNA or a pharmacological inhibitor decreased S1P levels in PC3-PR cells and inhibited proliferation in the presence or absence of PTX, suggesting that SPHK1 is at least partially responsible for PTX resistance. Paclitaxel 223-226 sphingosine kinase 1 Homo sapiens 14-19 28322796-6 2017 Inhibition of SPHK1 using siRNA or a pharmacological inhibitor decreased S1P levels in PC3-PR cells and inhibited proliferation in the presence or absence of PTX, suggesting that SPHK1 is at least partially responsible for PTX resistance. Paclitaxel 223-226 sphingosine kinase 1 Homo sapiens 179-184 28258651-5 2017 Additionally, SK1-expressing cells displayed a significant increase in glucose uptake paralleled by GLUT3 transporter upregulation. Glucose 71-78 sphingosine kinase 1 Homo sapiens 14-17 28284343-1 2017 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine1-phosphate (S1P), plays an important role in mediating post-stroke neuroinflammation. Sphingosine 75-86 sphingosine kinase 1 Homo sapiens 0-20 28284343-1 2017 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine1-phosphate (S1P), plays an important role in mediating post-stroke neuroinflammation. Sphingosine 75-86 sphingosine kinase 1 Homo sapiens 22-27 28284343-1 2017 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine1-phosphate (S1P), plays an important role in mediating post-stroke neuroinflammation. sphingosine 1-phosphate 92-114 sphingosine kinase 1 Homo sapiens 0-20 28284343-1 2017 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine1-phosphate (S1P), plays an important role in mediating post-stroke neuroinflammation. sphingosine 1-phosphate 92-114 sphingosine kinase 1 Homo sapiens 22-27 28284343-1 2017 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine1-phosphate (S1P), plays an important role in mediating post-stroke neuroinflammation. sphingosine 1-phosphate 116-119 sphingosine kinase 1 Homo sapiens 0-20 28284343-1 2017 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine1-phosphate (S1P), plays an important role in mediating post-stroke neuroinflammation. sphingosine 1-phosphate 116-119 sphingosine kinase 1 Homo sapiens 22-27 28284343-3 2017 In this study, we found that suppression of Sphk1 decreased IL17 production and relieved neuronal damage induced by microglia in cerebral ischemia reperfusion (IR) or in an in vitro oxygen-glucose deprivation reperfusion (OGDR) system. oxygen-glucose 182-196 sphingosine kinase 1 Homo sapiens 44-49 28454397-1 2017 Sphingosine kinase 1 (Sphk1) is an oncogenic kinase that is responsible for the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). Sphingosine 99-110 sphingosine kinase 1 Homo sapiens 0-20 28206952-0 2017 Identification of sphingosine kinase 1 (SphK1) as a primary target of icaritin in hepatocellular carcinoma cells. icaritin 70-78 sphingosine kinase 1 Homo sapiens 18-38 28206952-0 2017 Identification of sphingosine kinase 1 (SphK1) as a primary target of icaritin in hepatocellular carcinoma cells. icaritin 70-78 sphingosine kinase 1 Homo sapiens 40-45 28206952-4 2017 At the signaling level, icaritin inhibited sphingosine kinase 1 (SphK1) activity in HCC cells, which led to pro-apoptotic ceramide production and JNK1 activation. icaritin 24-32 sphingosine kinase 1 Homo sapiens 43-63 28206952-4 2017 At the signaling level, icaritin inhibited sphingosine kinase 1 (SphK1) activity in HCC cells, which led to pro-apoptotic ceramide production and JNK1 activation. icaritin 24-32 sphingosine kinase 1 Homo sapiens 65-70 28206952-4 2017 At the signaling level, icaritin inhibited sphingosine kinase 1 (SphK1) activity in HCC cells, which led to pro-apoptotic ceramide production and JNK1 activation. Ceramides 122-130 sphingosine kinase 1 Homo sapiens 43-63 28206952-4 2017 At the signaling level, icaritin inhibited sphingosine kinase 1 (SphK1) activity in HCC cells, which led to pro-apoptotic ceramide production and JNK1 activation. Ceramides 122-130 sphingosine kinase 1 Homo sapiens 65-70 28206952-5 2017 SphK1 inhibition or silence (by shRNA/microRNA) mimicked icaritin-mediated cytotoxicity, and almost nullified icaritin"s activity in HepG2 cells. icaritin 57-65 sphingosine kinase 1 Homo sapiens 0-5 28206952-5 2017 SphK1 inhibition or silence (by shRNA/microRNA) mimicked icaritin-mediated cytotoxicity, and almost nullified icaritin"s activity in HepG2 cells. icaritin 110-118 sphingosine kinase 1 Homo sapiens 0-5 28206952-6 2017 Reversely, exogenous over-expression of SphK1 sensitized icaritin-induced HepG2 cell apoptosis. icaritin 57-65 sphingosine kinase 1 Homo sapiens 40-45 28206952-8 2017 Further, SphK1 activity in icaritin-treated tumors was largely inhibited. icaritin 27-35 sphingosine kinase 1 Homo sapiens 9-14 28108260-9 2017 PF-543, a selective potent inhibitor of SphK1, attenuated epidermal growth factor (EGF)-mediated cell growth and survival signaling through inhibition of AKT, ERK, and p38 MAP kinase pathways mainly in LM2-4 cells but not in parental MDA-MB-231 human breast cancer cells. PF-543 0-6 sphingosine kinase 1 Homo sapiens 40-45 28454397-1 2017 Sphingosine kinase 1 (Sphk1) is an oncogenic kinase that is responsible for the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). Sphingosine 99-110 sphingosine kinase 1 Homo sapiens 22-27 28454397-1 2017 Sphingosine kinase 1 (Sphk1) is an oncogenic kinase that is responsible for the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 114-137 sphingosine kinase 1 Homo sapiens 0-20 28454397-1 2017 Sphingosine kinase 1 (Sphk1) is an oncogenic kinase that is responsible for the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 114-137 sphingosine kinase 1 Homo sapiens 22-27 28454397-4 2017 Therefore, the expression of Sphk1 was examined in 127 formalin-fixed, paraffin-embedded HCC tissues using immunohistochemistry, and its clinical implications and prognostic significance were analyzed. Formaldehyde 55-63 sphingosine kinase 1 Homo sapiens 29-34 28454397-4 2017 Therefore, the expression of Sphk1 was examined in 127 formalin-fixed, paraffin-embedded HCC tissues using immunohistochemistry, and its clinical implications and prognostic significance were analyzed. Paraffin 71-79 sphingosine kinase 1 Homo sapiens 29-34 27801960-6 2017 At both 4 and 6 weeks following CCl4 treatment, liver mRNA levels, protein concentration and immunohistochemical labelling for SphK1 increased significantly. Carbon Tetrachloride 32-36 sphingosine kinase 1 Homo sapiens 127-132 28231433-1 2017 Sphingosine kinase (SphK) is the major source of the lipid mediator and G protein-coupled receptor agonist sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 107-130 sphingosine kinase 1 Homo sapiens 0-18 28231433-1 2017 Sphingosine kinase (SphK) is the major source of the lipid mediator and G protein-coupled receptor agonist sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 107-130 sphingosine kinase 1 Homo sapiens 20-24 28231433-1 2017 Sphingosine kinase (SphK) is the major source of the lipid mediator and G protein-coupled receptor agonist sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 132-135 sphingosine kinase 1 Homo sapiens 0-18 28231433-1 2017 Sphingosine kinase (SphK) is the major source of the lipid mediator and G protein-coupled receptor agonist sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 132-135 sphingosine kinase 1 Homo sapiens 20-24 28386327-0 2017 Reduction of COX-2 through modulating miR-124/SPHK1 axis contributes to the antimetastatic effect of alpinumisoflavone in melanoma. alpinumisoflavone 101-118 sphingosine kinase 1 Homo sapiens 46-51 28351953-2 2017 SphK1 produces the signaling lipid sphingosine 1-phosphate (S1P), which activates signaling cascades downstream five G protein-coupled receptors (S1P1-5) to modulate vascular and immune system function and promote proliferation. sphingosine 1-phosphate 35-58 sphingosine kinase 1 Homo sapiens 0-5 27801960-10 2017 Furthermore, melatonin inhibited S1P production, lowered expression of SphK1, S1PR1, SP1R3, and ASMase, and increased expression of S1PL. Melatonin 13-22 sphingosine kinase 1 Homo sapiens 71-76 27801960-12 2017 Melatonin-treated cells also exhibited an inhibition of the SphK1/S1P axis. Melatonin 0-9 sphingosine kinase 1 Homo sapiens 60-65 27956387-2 2017 Sphingosine kinase 1 (SPHK1) generates the bioactive lipid sphingosine 1-phosphate (S1P) and has established roles in tumor initiation, progression, and chemotherapy resistance in a wide range of cancers. sphingosine 1-phosphate 59-82 sphingosine kinase 1 Homo sapiens 0-20 28231844-10 2017 CONCLUSION: Our data suggested that BMP4, MGP, TGFBI, and SPHK1 may be important in CAFs-associated NSCLC, and the abnormal expression and high LOH frequency of them may be used as the diagnosis targets of CAFs in NSCLC. cafs 84-88 sphingosine kinase 1 Homo sapiens 58-63 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Cholesterol 15-26 sphingosine kinase 1 Homo sapiens 128-148 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Cholesterol 15-26 sphingosine kinase 1 Homo sapiens 150-155 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Sphingomyelins 27-40 sphingosine kinase 1 Homo sapiens 128-148 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Sphingomyelins 27-40 sphingosine kinase 1 Homo sapiens 150-155 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Sphingolipids 197-209 sphingosine kinase 1 Homo sapiens 128-148 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Sphingolipids 197-209 sphingosine kinase 1 Homo sapiens 150-155 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Sphingosine 128-139 sphingosine kinase 1 Homo sapiens 150-155 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. sphingosine 1-phosphate 236-259 sphingosine kinase 1 Homo sapiens 128-148 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. sphingosine 1-phosphate 236-259 sphingosine kinase 1 Homo sapiens 150-155 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Sphingosine 221-232 sphingosine kinase 1 Homo sapiens 128-148 28049734-3 2017 Perturbing the cholesterol/sphingomyelin balance was shown to induce narrow tubular plasma membrane invaginations enriched with sphingosine kinase 1 (SphK1), the enzyme that converts the bioactive sphingolipid metabolite sphingosine to sphingosine-1-phosphate, and suggested a role for sphingosine phosphorylation in endocytic membrane trafficking. Sphingosine 221-232 sphingosine kinase 1 Homo sapiens 150-155 28049734-4 2017 Here we show that sphingosine and sphingosine-like SphK1 inhibitors induced rapid and massive formation of vesicles in diverse cell types that accumulated as dilated late endosomes. Sphingosine 34-45 sphingosine kinase 1 Homo sapiens 51-56 28049734-6 2017 Moreover, inhibition or deletion of SphK1 prolonged the lifetime of sphingosine-induced vesicles. Sphingosine 68-79 sphingosine kinase 1 Homo sapiens 36-41 28049734-9 2017 Together, our results highlight the importance of sphingosine and its conversion to sphingosine-1-phosphate by SphK1 in endocytic membrane trafficking. Sphingosine 50-61 sphingosine kinase 1 Homo sapiens 111-116 28049734-9 2017 Together, our results highlight the importance of sphingosine and its conversion to sphingosine-1-phosphate by SphK1 in endocytic membrane trafficking. sphingosine 1-phosphate 84-107 sphingosine kinase 1 Homo sapiens 111-116 27956387-2 2017 Sphingosine kinase 1 (SPHK1) generates the bioactive lipid sphingosine 1-phosphate (S1P) and has established roles in tumor initiation, progression, and chemotherapy resistance in a wide range of cancers. sphingosine 1-phosphate 59-82 sphingosine kinase 1 Homo sapiens 22-27 28165392-0 2017 Sphingosine Kinase-1 Involves the Inhibitory Action of HIF-1alpha by Chlorogenic Acid in Hypoxic DU145 Cells. Chlorogenic Acid 69-85 sphingosine kinase 1 Homo sapiens 0-20 28135860-1 2017 We have determined the gene expression of sphingosine-1-phosphate (S1P) metabolizing enzymes (SphK1, SphK2, SGPL1, SGPP1, SGPP2, PPAP2A, PPAP2B, and PPAP2C) by quantitative real-time polymerase chain reaction in tumor tissues and adjacent normal tissues of 50 oral squamous cell carcinoma (OSCC) patients. sphingosine 1-phosphate 42-65 sphingosine kinase 1 Homo sapiens 94-99 28075451-1 2017 Sphingosine kinase (SphK) is an important signalling enzyme that catalyses the phosphorylation of sphingosine (Sph) to form sphingosine-1-phosphate (S1P). Sphingosine 98-109 sphingosine kinase 1 Homo sapiens 0-18 28075451-1 2017 Sphingosine kinase (SphK) is an important signalling enzyme that catalyses the phosphorylation of sphingosine (Sph) to form sphingosine-1-phosphate (S1P). Sphingosine 98-109 sphingosine kinase 1 Homo sapiens 20-24 28075451-1 2017 Sphingosine kinase (SphK) is an important signalling enzyme that catalyses the phosphorylation of sphingosine (Sph) to form sphingosine-1-phosphate (S1P). Sphingosine 0-3 sphingosine kinase 1 Homo sapiens 20-24 28075451-1 2017 Sphingosine kinase (SphK) is an important signalling enzyme that catalyses the phosphorylation of sphingosine (Sph) to form sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 124-147 sphingosine kinase 1 Homo sapiens 0-18 28075451-1 2017 Sphingosine kinase (SphK) is an important signalling enzyme that catalyses the phosphorylation of sphingosine (Sph) to form sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 124-147 sphingosine kinase 1 Homo sapiens 20-24 27868302-4 2017 METHODS: Bisulfite sequencing was used to assess DNA methylation levels at CpG islands associated with genes encoding selected S1P system components, including sphingosine kinase 1 (SPHK1), S1PR1 and S1PR5, in alveolar macrophages from 20 COPD patients, 7 healthy smokers and 10 healthy non/ex-smokers) by methyl quantitative real-time PCR (methyl qPCR). hydrogen sulfite 9-18 sphingosine kinase 1 Homo sapiens 160-180 27141878-11 2017 Compound 3h negatively regulated activity of sphingosine kinase 1 in HeLa cells. Tritium 9-11 sphingosine kinase 1 Homo sapiens 45-65 28081222-7 2017 Further studies demonstrated that AT7867 inhibited sphingosine kinase 1 (SphK1) activity to promote pro-apoptotic ceramide production in HT-29 cells. AT 7867 34-40 sphingosine kinase 1 Homo sapiens 51-71 28081222-7 2017 Further studies demonstrated that AT7867 inhibited sphingosine kinase 1 (SphK1) activity to promote pro-apoptotic ceramide production in HT-29 cells. AT 7867 34-40 sphingosine kinase 1 Homo sapiens 73-78 28081222-7 2017 Further studies demonstrated that AT7867 inhibited sphingosine kinase 1 (SphK1) activity to promote pro-apoptotic ceramide production in HT-29 cells. Ceramides 114-122 sphingosine kinase 1 Homo sapiens 51-71 28081222-7 2017 Further studies demonstrated that AT7867 inhibited sphingosine kinase 1 (SphK1) activity to promote pro-apoptotic ceramide production in HT-29 cells. Ceramides 114-122 sphingosine kinase 1 Homo sapiens 73-78 27875779-5 2017 Cytostatic effect of pyrimidine-2,4-dione-1,2,3-triazole hybrid 7 in HepG2 cells could be attributed to the Wee-1 kinase inhibition and abolishment of sphingolipid signaling mediated by acid ceramidase and sphingosine kinase 1. pyrimidine-2,4-dione-1,2,3-triazole 21-56 sphingosine kinase 1 Homo sapiens 206-226 27821815-0 2016 Everolimus (RAD001) sensitizes prostate cancer cells to docetaxel by down-regulation of HIF-1alpha and sphingosine kinase 1. Everolimus 0-10 sphingosine kinase 1 Homo sapiens 103-123 28270071-9 2017 RESULTS: SG12 and SG14 mutually showed stronger inhibitory effects with less cytotoxicity compared with a general SphK inhibitor, N,N-dimethylsphingosine (DMS). N,N-dimethylsphingosine 155-158 sphingosine kinase 1 Homo sapiens 114-118 27864331-3 2016 Here, we report a role for HGF-induced intracellular sphingosine-1-phosphate (S1P) generation catalyzed by sphingosine kinase 1 (SphK1), S1P transporter, spinster homolog 2 (Spns2), and S1P receptor, S1P1, in lamellipodia formation and perhaps motility of HLMVECs. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 107-127 27864331-3 2016 Here, we report a role for HGF-induced intracellular sphingosine-1-phosphate (S1P) generation catalyzed by sphingosine kinase 1 (SphK1), S1P transporter, spinster homolog 2 (Spns2), and S1P receptor, S1P1, in lamellipodia formation and perhaps motility of HLMVECs. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 129-134 28479957-6 2017 The formation of tubes by 4-HNE involved the generation of reactive oxygen species and the activation of the sphingolipid pathway, namely, the neutral type 2 sphingomyelinase and sphingosine kinase-1 (nSMase2/SK-1) pathway, indicating a role for S1P in the angiogenic signaling of 4-HNE. 4-hydroxy-2-nonenal 26-31 sphingosine kinase 1 Homo sapiens 179-199 28479957-6 2017 The formation of tubes by 4-HNE involved the generation of reactive oxygen species and the activation of the sphingolipid pathway, namely, the neutral type 2 sphingomyelinase and sphingosine kinase-1 (nSMase2/SK-1) pathway, indicating a role for S1P in the angiogenic signaling of 4-HNE. 4-hydroxy-2-nonenal 26-31 sphingosine kinase 1 Homo sapiens 209-213 28479957-6 2017 The formation of tubes by 4-HNE involved the generation of reactive oxygen species and the activation of the sphingolipid pathway, namely, the neutral type 2 sphingomyelinase and sphingosine kinase-1 (nSMase2/SK-1) pathway, indicating a role for S1P in the angiogenic signaling of 4-HNE. Sphingolipids 109-121 sphingosine kinase 1 Homo sapiens 179-199 28479957-6 2017 The formation of tubes by 4-HNE involved the generation of reactive oxygen species and the activation of the sphingolipid pathway, namely, the neutral type 2 sphingomyelinase and sphingosine kinase-1 (nSMase2/SK-1) pathway, indicating a role for S1P in the angiogenic signaling of 4-HNE. Sphingolipids 109-121 sphingosine kinase 1 Homo sapiens 209-213 28479957-6 2017 The formation of tubes by 4-HNE involved the generation of reactive oxygen species and the activation of the sphingolipid pathway, namely, the neutral type 2 sphingomyelinase and sphingosine kinase-1 (nSMase2/SK-1) pathway, indicating a role for S1P in the angiogenic signaling of 4-HNE. 4-hydroxy-2-nonenal 281-286 sphingosine kinase 1 Homo sapiens 179-199 28479957-6 2017 The formation of tubes by 4-HNE involved the generation of reactive oxygen species and the activation of the sphingolipid pathway, namely, the neutral type 2 sphingomyelinase and sphingosine kinase-1 (nSMase2/SK-1) pathway, indicating a role for S1P in the angiogenic signaling of 4-HNE. 4-hydroxy-2-nonenal 281-286 sphingosine kinase 1 Homo sapiens 209-213 28479957-7 2017 Carbonyl scavengers hydralazine and bisvanillyl-hydralazone inhibited the nSMase2/SK1 pathway activation and the formation of tubes on Matrigel evoked by 4-HNE. Hydralazine 20-31 sphingosine kinase 1 Homo sapiens 82-85 28479957-7 2017 Carbonyl scavengers hydralazine and bisvanillyl-hydralazone inhibited the nSMase2/SK1 pathway activation and the formation of tubes on Matrigel evoked by 4-HNE. bisvanillyl-hydralazone 36-59 sphingosine kinase 1 Homo sapiens 82-85 27821815-0 2016 Everolimus (RAD001) sensitizes prostate cancer cells to docetaxel by down-regulation of HIF-1alpha and sphingosine kinase 1. Docetaxel 56-65 sphingosine kinase 1 Homo sapiens 103-123 27821815-6 2016 SK1 overexpression has completely protected prostate cancer cells from RAD001/docetaxel effects. Docetaxel 78-87 sphingosine kinase 1 Homo sapiens 0-3 27821815-7 2016 Using gene knockdown and CoCl2 treatment we showed that SK1 mRNA expression is downstream of HIF-1alpha. cobaltous chloride 25-30 sphingosine kinase 1 Homo sapiens 56-59 27821815-9 2016 RAD001 alone or in combination with docetaxel has suppressed intratumoral mTOR and SK1 signaling, however as evidenced by tumor size, it required docetaxel for clinical efficacy. Docetaxel 36-45 sphingosine kinase 1 Homo sapiens 83-86 27621206-3 2016 Sphingosine Kinases (SphKs) 1 and 2, catalyze the ATP-dependent phosphorylation of sphingosine to S1P, while the degradation is mediated by the reversible dephosphorylation catalyzed by the S1P phosphatases and lipid phosphate phosphatases and the irreversible degradation to hexadecenal and ethanolamine phosphate by sphingosine-1-phosphate lyase (S1PL). Adenosine Triphosphate 50-53 sphingosine kinase 1 Homo sapiens 0-35 27539678-1 2016 INTRODUCTION: Sphingosine kinase (SphK1 & SphK2) is the sole source of the pleiotropic lipid mediator, sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 107-130 sphingosine kinase 1 Homo sapiens 34-39 27621206-3 2016 Sphingosine Kinases (SphKs) 1 and 2, catalyze the ATP-dependent phosphorylation of sphingosine to S1P, while the degradation is mediated by the reversible dephosphorylation catalyzed by the S1P phosphatases and lipid phosphate phosphatases and the irreversible degradation to hexadecenal and ethanolamine phosphate by sphingosine-1-phosphate lyase (S1PL). Sphingosine 83-94 sphingosine kinase 1 Homo sapiens 0-35 27621206-3 2016 Sphingosine Kinases (SphKs) 1 and 2, catalyze the ATP-dependent phosphorylation of sphingosine to S1P, while the degradation is mediated by the reversible dephosphorylation catalyzed by the S1P phosphatases and lipid phosphate phosphatases and the irreversible degradation to hexadecenal and ethanolamine phosphate by sphingosine-1-phosphate lyase (S1PL). (E)-2-hexadecenal 276-287 sphingosine kinase 1 Homo sapiens 0-35 27621206-3 2016 Sphingosine Kinases (SphKs) 1 and 2, catalyze the ATP-dependent phosphorylation of sphingosine to S1P, while the degradation is mediated by the reversible dephosphorylation catalyzed by the S1P phosphatases and lipid phosphate phosphatases and the irreversible degradation to hexadecenal and ethanolamine phosphate by sphingosine-1-phosphate lyase (S1PL). phosphorylethanolamine 292-314 sphingosine kinase 1 Homo sapiens 0-35 27708249-3 2016 We previously reported an increased expression of sphingosine kinase-1 (SK1), which produces the bioactive lipid sphingosine 1-phosphate (S1P), in melanoma. sphingosine 1-phosphate 113-136 sphingosine kinase 1 Homo sapiens 50-75 27904661-2 2016 SKI-5C is a new SphK1 inhibitor, and until now its molecular function in Wilms" tumor cells remained unknown. SKI 5C 0-6 sphingosine kinase 1 Homo sapiens 16-21 27904661-4 2016 We demonstrated that SphK1 is highly expressed in SK-NEP-1 and G401 cells, and through annexin V/propidium iodide staining and flow cytometry analysis, we detected cell apoptosis. Propidium 97-113 sphingosine kinase 1 Homo sapiens 21-26 27806293-2 2016 Here, we demonstrate that the recruitment of Sphk1 to sphingosine-enriched endocytic vesicles and the generation of sphingosine-1-phosphate facilitate membrane trafficking along the endosomal pathway. Sphingosine 54-65 sphingosine kinase 1 Homo sapiens 45-50 27806293-2 2016 Here, we demonstrate that the recruitment of Sphk1 to sphingosine-enriched endocytic vesicles and the generation of sphingosine-1-phosphate facilitate membrane trafficking along the endosomal pathway. sphingosine 1-phosphate 116-139 sphingosine kinase 1 Homo sapiens 45-50 27806293-3 2016 Exogenous sphingosine and sphingosine-based Sphk1 inhibitors induce the Sphk1-dependent fusion of endosomal membranes to accumulate enlarged late endosomes and amphisomes enriched in sphingolipids. Sphingosine 10-21 sphingosine kinase 1 Homo sapiens 72-77 27806293-3 2016 Exogenous sphingosine and sphingosine-based Sphk1 inhibitors induce the Sphk1-dependent fusion of endosomal membranes to accumulate enlarged late endosomes and amphisomes enriched in sphingolipids. Sphingosine 26-37 sphingosine kinase 1 Homo sapiens 44-49 27806293-3 2016 Exogenous sphingosine and sphingosine-based Sphk1 inhibitors induce the Sphk1-dependent fusion of endosomal membranes to accumulate enlarged late endosomes and amphisomes enriched in sphingolipids. Sphingosine 26-37 sphingosine kinase 1 Homo sapiens 72-77 27806293-3 2016 Exogenous sphingosine and sphingosine-based Sphk1 inhibitors induce the Sphk1-dependent fusion of endosomal membranes to accumulate enlarged late endosomes and amphisomes enriched in sphingolipids. Sphingolipids 183-196 sphingosine kinase 1 Homo sapiens 44-49 27806293-3 2016 Exogenous sphingosine and sphingosine-based Sphk1 inhibitors induce the Sphk1-dependent fusion of endosomal membranes to accumulate enlarged late endosomes and amphisomes enriched in sphingolipids. Sphingolipids 183-196 sphingosine kinase 1 Homo sapiens 72-77 27806293-4 2016 Interestingly, Sphk1 also appears to facilitate endosomal fusion independent of its catalytic activity, given that catalytically inactive Sphk1G82D is recruited to endocytic membranes by sphingosine or sphingosine-based Sphk1 inhibitor and promotes membrane fusion. Sphingosine 187-198 sphingosine kinase 1 Homo sapiens 15-20 27806293-4 2016 Interestingly, Sphk1 also appears to facilitate endosomal fusion independent of its catalytic activity, given that catalytically inactive Sphk1G82D is recruited to endocytic membranes by sphingosine or sphingosine-based Sphk1 inhibitor and promotes membrane fusion. Sphingosine 187-198 sphingosine kinase 1 Homo sapiens 138-143 27806293-4 2016 Interestingly, Sphk1 also appears to facilitate endosomal fusion independent of its catalytic activity, given that catalytically inactive Sphk1G82D is recruited to endocytic membranes by sphingosine or sphingosine-based Sphk1 inhibitor and promotes membrane fusion. Sphingosine 202-213 sphingosine kinase 1 Homo sapiens 15-20 27806293-4 2016 Interestingly, Sphk1 also appears to facilitate endosomal fusion independent of its catalytic activity, given that catalytically inactive Sphk1G82D is recruited to endocytic membranes by sphingosine or sphingosine-based Sphk1 inhibitor and promotes membrane fusion. Sphingosine 202-213 sphingosine kinase 1 Homo sapiens 138-143 27505892-5 2016 Recent studies have shown that elevated sphingosine kinase 1 (Sphk1)-mediated sphingosine 1-phosphate production contributes to sickling. sphingosine 1-phosphate 78-101 sphingosine kinase 1 Homo sapiens 40-60 27588496-1 2016 While both human sphingosine kinases (SK1 and SK2) catalyze the generation of the pleiotropic signaling lipid sphingosine 1-phosphate, these enzymes appear to be functionally distinct. sphingosine 1-phosphate 110-133 sphingosine kinase 1 Homo sapiens 38-41 27505892-5 2016 Recent studies have shown that elevated sphingosine kinase 1 (Sphk1)-mediated sphingosine 1-phosphate production contributes to sickling. sphingosine 1-phosphate 78-101 sphingosine kinase 1 Homo sapiens 62-67 27063355-1 2016 Recent studies have demonstrated that the expression of sphingosine kinase 1, the enzyme that catalyses formation of the bioactive lipid, sphingosine 1-phosphate, is increased in lungs from patients with pulmonary arterial hypertension. sphingosine 1-phosphate 138-161 sphingosine kinase 1 Homo sapiens 56-76 27621003-0 2016 Breast cancer sphingosine-1-phosphate is associated with phospho-sphingosine kinase 1 and lymphatic metastasis. sphingosine 1-phosphate 14-37 sphingosine kinase 1 Homo sapiens 65-85 27467777-3 2016 SPHK1 synthesizes sphingosine-1-phosphate (S1P), a bioactive lipid involved in cell survival. sphingosine 1-phosphate 18-41 sphingosine kinase 1 Homo sapiens 0-5 27467777-5 2016 Interestingly, in non-neuronal cells stimulated with growth factors, SPHK1 translocates to the plasma membrane, where it phosphorylates sphingosine to produce S1P. Sphingosine 136-147 sphingosine kinase 1 Homo sapiens 69-74 27297359-1 2016 Sphingosine kinase (SK) 1 and 2 are lipid kinases that catalyse the formation of sphingosine 1-phosphate (S1P), a potent signalling molecule with a wide array of cellular effects. sphingosine 1-phosphate 81-104 sphingosine kinase 1 Homo sapiens 0-31 27101794-1 2016 The sphingosine kinase (SphK)1/sphingosine-1-phosphate (S1P) pathway is involved in multiple biological processes, including liver diseases. sphingosine 1-phosphate 31-54 sphingosine kinase 1 Homo sapiens 4-30 27101794-2 2016 This study investigate whether modulation of the SphK1/S1P system associates to the beneficial effects of melatonin in an animal model of acute liver failure (ALF) induced by the rabbit hemorrhagic disease virus (RHDV). Melatonin 106-115 sphingosine kinase 1 Homo sapiens 49-54 27581969-0 2016 Anti-cancer effect of pristimerin by inhibition of HIF-1alpha involves the SPHK-1 pathway in hypoxic prostate cancer cells. pristimerin 22-33 sphingosine kinase 1 Homo sapiens 75-81 27581969-5 2016 METHODS: To investigate the molecular mechanisms of pristimerin in association with SPHK-1 pathways in hypoxic PC-3 cancer cells. pristimerin 52-63 sphingosine kinase 1 Homo sapiens 84-90 27581969-8 2016 Pristimerin suppressed the expression of HIF-1alpha by inhibiting SPHK-1. pristimerin 0-11 sphingosine kinase 1 Homo sapiens 66-72 27581969-9 2016 Moreover, inhibiting SPHK-1 with a sphingosine kinase inhibitor enhanced the suppression of HIF-1alpha, phosphorylation AKT, and glycogen synthase kinase-3beta (GSK-3beta) by pristimerin under hypoxia. pristimerin 175-186 sphingosine kinase 1 Homo sapiens 21-27 27581969-10 2016 Furthermore, a reactive oxygen species (ROS) scavenger enhanced the inhibition of HIF-1alpha and SPHK-1 by pristimerin. Reactive Oxygen Species 15-38 sphingosine kinase 1 Homo sapiens 97-103 27581969-10 2016 Furthermore, a reactive oxygen species (ROS) scavenger enhanced the inhibition of HIF-1alpha and SPHK-1 by pristimerin. Reactive Oxygen Species 40-43 sphingosine kinase 1 Homo sapiens 97-103 27581969-10 2016 Furthermore, a reactive oxygen species (ROS) scavenger enhanced the inhibition of HIF-1alpha and SPHK-1 by pristimerin. pristimerin 107-118 sphingosine kinase 1 Homo sapiens 97-103 27581969-11 2016 CONCLUSION: Taken together, these findings suggest that pristimerin can exert an anti-cancer activity by inhibiting HIF-1alpha through the SPHK-1 pathway. pristimerin 56-67 sphingosine kinase 1 Homo sapiens 139-145 27349500-0 2016 Atorvastatin partially inhibits the epithelial-mesenchymal transition in A549 cells induced by TGF-beta1 by attenuating the upregulation of SphK1. Atorvastatin 0-12 sphingosine kinase 1 Homo sapiens 140-145 27349500-8 2016 SphK1 plasmid transient transfection strengthened the EMT process induced by TGF-beta1 in the presence of atorvastatin. Atorvastatin 106-118 sphingosine kinase 1 Homo sapiens 0-5 27349500-9 2016 Our experiments confirmed that atorvastatin can partially inhibit the EMT process of non-small cell lung cancer cells induced by TGF-beta1 by attenuating the upregulation of SphK1. Atorvastatin 31-43 sphingosine kinase 1 Homo sapiens 174-179 27255176-2 2016 In recent study we reported the N"-(3-(benzyloxy)benzylidene)-3,4,5-trihydroxybenzohydrazide scaffold as a potent SphK inhibitor. n"-(3-(benzyloxy)benzylidene)-3,4,5-trihydroxybenzohydrazide 32-92 sphingosine kinase 1 Homo sapiens 114-118 27284992-5 2016 Furthermore, in in vitro (JAR cells) and ex vivo (chorionic villous explants) models of placental hypoxia, SPHK1 mRNA and protein were strongly up-regulated under low oxygen tension (1% 02). Oxygen 167-173 sphingosine kinase 1 Homo sapiens 107-112 27255176-1 2016 Sphingosine kinase (SphK)-catalyzed production of sphingosine-1-phosphate (S1P) regulates cell growth, survival and proliferation as well as inflammatory status in animals. sphingosine 1-phosphate 50-73 sphingosine kinase 1 Homo sapiens 20-24 27255176-1 2016 Sphingosine kinase (SphK)-catalyzed production of sphingosine-1-phosphate (S1P) regulates cell growth, survival and proliferation as well as inflammatory status in animals. sphingosine 1-phosphate 75-78 sphingosine kinase 1 Homo sapiens 20-24 27002656-0 2016 Sphingosine kinase 1/sphingosine-1-phosphate regulates the expression of interleukin-17A in activated microglia in cerebral ischemia/reperfusion. sphingosine 1-phosphate 21-44 sphingosine kinase 1 Homo sapiens 0-20 27002656-2 2016 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine-1-phosphate (S1P), plays an important role in the regulation of proinflammatory cytokines in activated microglia. Sphingosine 75-86 sphingosine kinase 1 Homo sapiens 0-20 27002656-2 2016 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine-1-phosphate (S1P), plays an important role in the regulation of proinflammatory cytokines in activated microglia. Sphingosine 75-86 sphingosine kinase 1 Homo sapiens 22-27 27002656-2 2016 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine-1-phosphate (S1P), plays an important role in the regulation of proinflammatory cytokines in activated microglia. sphingosine 1-phosphate 92-115 sphingosine kinase 1 Homo sapiens 0-20 27002656-2 2016 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine-1-phosphate (S1P), plays an important role in the regulation of proinflammatory cytokines in activated microglia. sphingosine 1-phosphate 92-115 sphingosine kinase 1 Homo sapiens 22-27 27002656-2 2016 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine-1-phosphate (S1P), plays an important role in the regulation of proinflammatory cytokines in activated microglia. sphingosine 1-phosphate 117-120 sphingosine kinase 1 Homo sapiens 0-20 27002656-2 2016 Sphingosine kinase 1 (Sphk1), a key enzyme responsible for phosphorylating sphingosine into sphingosine-1-phosphate (S1P), plays an important role in the regulation of proinflammatory cytokines in activated microglia. sphingosine 1-phosphate 117-120 sphingosine kinase 1 Homo sapiens 22-27 27002656-10 2016 Pre-injection with PF-543, an inhibitor of Sphk1, before IR clearly reduced the expression of Sphk1 in microglia relative to brain IR alone. PF-543 19-25 sphingosine kinase 1 Homo sapiens 43-48 27002656-10 2016 Pre-injection with PF-543, an inhibitor of Sphk1, before IR clearly reduced the expression of Sphk1 in microglia relative to brain IR alone. PF-543 19-25 sphingosine kinase 1 Homo sapiens 94-99 26792820-3 2016 Measurement of sphingosine kinase 1/2 activities by [(32)P]-labeled sphingosine-1-phosphate revealed a 30% reduction of sphingosine kinase 1 (P < 0.05) and a nonsignificant decrease of sphingosine kinase 2 in THP-1 macrophages after 1 h cigarette smoke extract exposure. Phosphorus-32 52-59 sphingosine kinase 1 Homo sapiens 15-37 26792820-3 2016 Measurement of sphingosine kinase 1/2 activities by [(32)P]-labeled sphingosine-1-phosphate revealed a 30% reduction of sphingosine kinase 1 (P < 0.05) and a nonsignificant decrease of sphingosine kinase 2 in THP-1 macrophages after 1 h cigarette smoke extract exposure. Phosphorus-32 52-59 sphingosine kinase 1 Homo sapiens 15-35 26792820-3 2016 Measurement of sphingosine kinase 1/2 activities by [(32)P]-labeled sphingosine-1-phosphate revealed a 30% reduction of sphingosine kinase 1 (P < 0.05) and a nonsignificant decrease of sphingosine kinase 2 in THP-1 macrophages after 1 h cigarette smoke extract exposure. sphingosine 1-phosphate 68-91 sphingosine kinase 1 Homo sapiens 15-37 26792820-3 2016 Measurement of sphingosine kinase 1/2 activities by [(32)P]-labeled sphingosine-1-phosphate revealed a 30% reduction of sphingosine kinase 1 (P < 0.05) and a nonsignificant decrease of sphingosine kinase 2 in THP-1 macrophages after 1 h cigarette smoke extract exposure. sphingosine 1-phosphate 68-91 sphingosine kinase 1 Homo sapiens 15-35 26792820-7 2016 Cigarette smoke-inhibited efferocytosis was significantly (P < 0.05) reversed to near-control levels in the presence of 10-100 nM exogenous sphingosine-1-phosphate or FTY720, and FTY720 reduced cigarette smoke-induced clumping of sphingosine kinase 1/2 in the Golgi domain. sphingosine 1-phosphate 143-166 sphingosine kinase 1 Homo sapiens 233-255 26923291-4 2016 In this study, we elucidated that Sphk1/S1P upregulates sirtuin 1 (SIRT1), a NAD+ dependent deacetylases protease which exerts multiple cellular functions, to regulate the proliferation and migration of endothelial cells. NAD 77-80 sphingosine kinase 1 Homo sapiens 34-39 27099350-3 2016 Recently, the bioactive lipid sphingosine-1-phosphate (S1P) and its activating kinase, sphingosine kinase 1 (SphK1), have been shown to be upregulated in PAH and promote PASMC proliferation. sphingosine 1-phosphate 30-53 sphingosine kinase 1 Homo sapiens 87-107 27099350-3 2016 Recently, the bioactive lipid sphingosine-1-phosphate (S1P) and its activating kinase, sphingosine kinase 1 (SphK1), have been shown to be upregulated in PAH and promote PASMC proliferation. sphingosine 1-phosphate 30-53 sphingosine kinase 1 Homo sapiens 109-114 27099350-3 2016 Recently, the bioactive lipid sphingosine-1-phosphate (S1P) and its activating kinase, sphingosine kinase 1 (SphK1), have been shown to be upregulated in PAH and promote PASMC proliferation. pasmc 170-175 sphingosine kinase 1 Homo sapiens 87-107 27129165-0 2016 Benzyl butyl phthalate promotes breast cancer stem cell expansion via SPHK1/S1P/S1PR3 signaling. butylbenzyl phthalate 0-22 sphingosine kinase 1 Homo sapiens 70-75 27129165-3 2016 A toxic phthalate, benzyl butyl phthalate (BBP), activated aryl hydrocarbon receptor in breast cancer cells to stimulate sphingosine kinase 1 (SPHK1)/sphingosine 1-phosphate (S1P)/sphingosine-1-phosphate receptor 3 (S1PR3) signaling and enhance formation of metastasis-initiating BCSCs. phthalic acid 8-17 sphingosine kinase 1 Homo sapiens 121-141 27129165-3 2016 A toxic phthalate, benzyl butyl phthalate (BBP), activated aryl hydrocarbon receptor in breast cancer cells to stimulate sphingosine kinase 1 (SPHK1)/sphingosine 1-phosphate (S1P)/sphingosine-1-phosphate receptor 3 (S1PR3) signaling and enhance formation of metastasis-initiating BCSCs. phthalic acid 8-17 sphingosine kinase 1 Homo sapiens 143-148 27129165-3 2016 A toxic phthalate, benzyl butyl phthalate (BBP), activated aryl hydrocarbon receptor in breast cancer cells to stimulate sphingosine kinase 1 (SPHK1)/sphingosine 1-phosphate (S1P)/sphingosine-1-phosphate receptor 3 (S1PR3) signaling and enhance formation of metastasis-initiating BCSCs. butylbenzyl phthalate 19-41 sphingosine kinase 1 Homo sapiens 121-141 27129165-3 2016 A toxic phthalate, benzyl butyl phthalate (BBP), activated aryl hydrocarbon receptor in breast cancer cells to stimulate sphingosine kinase 1 (SPHK1)/sphingosine 1-phosphate (S1P)/sphingosine-1-phosphate receptor 3 (S1PR3) signaling and enhance formation of metastasis-initiating BCSCs. butylbenzyl phthalate 19-41 sphingosine kinase 1 Homo sapiens 143-148 27129165-3 2016 A toxic phthalate, benzyl butyl phthalate (BBP), activated aryl hydrocarbon receptor in breast cancer cells to stimulate sphingosine kinase 1 (SPHK1)/sphingosine 1-phosphate (S1P)/sphingosine-1-phosphate receptor 3 (S1PR3) signaling and enhance formation of metastasis-initiating BCSCs. butylbenzyl phthalate 43-46 sphingosine kinase 1 Homo sapiens 121-141 27129165-3 2016 A toxic phthalate, benzyl butyl phthalate (BBP), activated aryl hydrocarbon receptor in breast cancer cells to stimulate sphingosine kinase 1 (SPHK1)/sphingosine 1-phosphate (S1P)/sphingosine-1-phosphate receptor 3 (S1PR3) signaling and enhance formation of metastasis-initiating BCSCs. butylbenzyl phthalate 43-46 sphingosine kinase 1 Homo sapiens 143-148 27099350-3 2016 Recently, the bioactive lipid sphingosine-1-phosphate (S1P) and its activating kinase, sphingosine kinase 1 (SphK1), have been shown to be upregulated in PAH and promote PASMC proliferation. pasmc 170-175 sphingosine kinase 1 Homo sapiens 109-114 27001955-7 2016 The present study reveals that when applied topically, fingolimod attenuates both immediate and late-phase responses to histamine with reduced extravasation of fluid, SK-1 activity, proinflammatory cytokine and chemokine production, and neutrophil influx and prevents ear swelling. Fingolimod Hydrochloride 55-65 sphingosine kinase 1 Homo sapiens 167-171 27021309-1 2016 Sphingosine kinases (SK1 and SK2) catalyse the conversion of sphingosine into sphingosine 1-phosphate and control fundamental cellular processes, including cell survival, proliferation, differentiation, migration, and immune function. Sphingosine 61-72 sphingosine kinase 1 Homo sapiens 21-24 27021309-1 2016 Sphingosine kinases (SK1 and SK2) catalyse the conversion of sphingosine into sphingosine 1-phosphate and control fundamental cellular processes, including cell survival, proliferation, differentiation, migration, and immune function. sphingosine 1-phosphate 78-101 sphingosine kinase 1 Homo sapiens 21-24 26920060-7 2016 Further, A-674563 decreased sphingosine kinase 1 (SphK1) activity in above AML cells to deplete pro-survival sphingosine-1-phosphate (S1P) and boost pro-apoptotic ceramide production. sphingosine 1-phosphate 109-132 sphingosine kinase 1 Homo sapiens 28-48 26662312-2 2016 Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers and may act as an oncogene in tumorigenesis. Sphingosine 51-62 sphingosine kinase 1 Homo sapiens 0-20 26662312-2 2016 Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers and may act as an oncogene in tumorigenesis. Sphingosine 51-62 sphingosine kinase 1 Homo sapiens 22-27 26662312-2 2016 Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers and may act as an oncogene in tumorigenesis. sphingosine 1-phosphate 66-89 sphingosine kinase 1 Homo sapiens 0-20 26662312-2 2016 Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers and may act as an oncogene in tumorigenesis. sphingosine 1-phosphate 66-89 sphingosine kinase 1 Homo sapiens 22-27 26920060-7 2016 Further, A-674563 decreased sphingosine kinase 1 (SphK1) activity in above AML cells to deplete pro-survival sphingosine-1-phosphate (S1P) and boost pro-apoptotic ceramide production. sphingosine 1-phosphate 109-132 sphingosine kinase 1 Homo sapiens 50-55 26920060-7 2016 Further, A-674563 decreased sphingosine kinase 1 (SphK1) activity in above AML cells to deplete pro-survival sphingosine-1-phosphate (S1P) and boost pro-apoptotic ceramide production. Ceramides 163-171 sphingosine kinase 1 Homo sapiens 50-55 26920060-9 2016 Significantly, K6PC-5, a novel SphK1 activator, or supplement with S1P attenuated A-674563-induced ceramide production, and subsequent U937 cell death and apoptosis. Ceramides 99-107 sphingosine kinase 1 Homo sapiens 31-36 26944438-2 2016 Sphingosine kinase 1 (SphK1), which converts sphingosine to S1P, is a key promoter in cancer. Sphingosine 45-56 sphingosine kinase 1 Homo sapiens 0-20 26944438-2 2016 Sphingosine kinase 1 (SphK1), which converts sphingosine to S1P, is a key promoter in cancer. Sphingosine 45-56 sphingosine kinase 1 Homo sapiens 22-27 26934645-10 2016 Therefore, we propose targeting androgen-independent prostate cancer cells with compounds that affect Des1/SK1 to modulate both de novo and sphingolipid rheostat pathways in order to induce growth arrest. Sphingolipids 140-152 sphingosine kinase 1 Homo sapiens 107-110 26855418-11 2016 In conclusion, low oxLDL concentration triggers sprouting angiogenesis that involves ROS-induced activation of the neutral sphingomyelinase-2/sphingosine kinase-1 pathway, and is effectively inhibited by GW4869. Reactive Oxygen Species 85-88 sphingosine kinase 1 Homo sapiens 142-162 26855418-11 2016 In conclusion, low oxLDL concentration triggers sprouting angiogenesis that involves ROS-induced activation of the neutral sphingomyelinase-2/sphingosine kinase-1 pathway, and is effectively inhibited by GW4869. GW 4869 204-210 sphingosine kinase 1 Homo sapiens 142-162 26927311-2 2016 Sphingosine kinases (SKs) including SK1 and SK2 phosphorylate sphingosine to sphingosine 1-phosphate (S1P), and control the critical balance. Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 36-39 26927311-2 2016 Sphingosine kinases (SKs) including SK1 and SK2 phosphorylate sphingosine to sphingosine 1-phosphate (S1P), and control the critical balance. sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 36-39 26927311-2 2016 Sphingosine kinases (SKs) including SK1 and SK2 phosphorylate sphingosine to sphingosine 1-phosphate (S1P), and control the critical balance. sphingosine 1-phosphate 102-105 sphingosine kinase 1 Homo sapiens 36-39 26970273-1 2016 Sphingosine kinases (isoforms SK1 and SK2) catalyse the formation of a bioactive lipid, sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 88-111 sphingosine kinase 1 Homo sapiens 30-33 26970273-1 2016 Sphingosine kinases (isoforms SK1 and SK2) catalyse the formation of a bioactive lipid, sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 113-116 sphingosine kinase 1 Homo sapiens 30-33 26970273-7 2016 Analysis of the crystal structure of SK1 with the SK1-selective inhibitor, PF-543, is used to identify residues that could be exploited to improve selectivity in SK inhibitor development for future therapeutic application. PF-543 75-81 sphingosine kinase 1 Homo sapiens 37-40 26970273-7 2016 Analysis of the crystal structure of SK1 with the SK1-selective inhibitor, PF-543, is used to identify residues that could be exploited to improve selectivity in SK inhibitor development for future therapeutic application. PF-543 75-81 sphingosine kinase 1 Homo sapiens 50-53 26934645-1 2016 Sphingosine kinases (two isoforms termed SK1 and SK2) catalyse the formation of the bioactive lipid sphingosine 1-phosphate. sphingosine 1-phosphate 100-123 sphingosine kinase 1 Homo sapiens 41-44 27190598-3 2016 Recently, we reported a series of amidine-based inhibitors with high selectivity for SphK1 and potency in the nanomolar range. Amidines 34-41 sphingosine kinase 1 Homo sapiens 85-90 27190598-6 2016 Generation of a library of molecules resulted in the identification of the most selective inhibitor of SphK1 reported to date (705-fold selectivity over SphK2), and we found that potency and selectivity vary significantly depending on the particular oxadiazole isomer employed. Oxadiazoles 250-260 sphingosine kinase 1 Homo sapiens 103-108 27190598-7 2016 The best inhibitors were subjected to in silico molecular dynamics docking analysis, which revealed key insights into the binding of amidine-based inhibitors by SphK1. Amidines 133-140 sphingosine kinase 1 Homo sapiens 161-166 26937138-1 2016 AIM: To examine the expression of SphK1, an oncogenic kinase that produces sphingosine 1-phosphate (S1P), and its correlation with the expression of LPAR2, a major lysophosphatidic acid (LPA) receptor overexpressed in various cancers, in human colorectal cancer. sphingosine 1-phosphate 75-98 sphingosine kinase 1 Homo sapiens 34-39 26985306-1 2016 The two isoforms of sphingosine kinase (SphK1 and SphK2) are the only enzymes that phosphorylate sphingosine to sphingosine-1-phosphate (S1P), which is a pleiotropic lipid mediator involved in a broad range of cellular processes including migration, proliferation, and inflammation. Sphingosine 20-31 sphingosine kinase 1 Homo sapiens 40-45 26877098-3 2016 In this study, we report that secretion of spingsine-1-phosphate (S1P) and its regulator, SphK1 expression is dependent of the substrate rigidity, which is critical for the balance between cancer cell invasion and adhesion. spingsine-1-phosphate 43-64 sphingosine kinase 1 Homo sapiens 90-95 26985306-1 2016 The two isoforms of sphingosine kinase (SphK1 and SphK2) are the only enzymes that phosphorylate sphingosine to sphingosine-1-phosphate (S1P), which is a pleiotropic lipid mediator involved in a broad range of cellular processes including migration, proliferation, and inflammation. sphingosine 1-phosphate 112-135 sphingosine kinase 1 Homo sapiens 40-45 26985306-3 2016 Herein, we disclose the structure-activity profile of naphthalene-containing SphK inhibitors and molecular modeling studies that reveal a key molecular switch that controls SphK selectivity. naphthalene 54-65 sphingosine kinase 1 Homo sapiens 77-81 26985306-3 2016 Herein, we disclose the structure-activity profile of naphthalene-containing SphK inhibitors and molecular modeling studies that reveal a key molecular switch that controls SphK selectivity. naphthalene 54-65 sphingosine kinase 1 Homo sapiens 173-177 26376826-3 2016 Recent studies showed that sphingosine kinase/sphingosine-1-phosphate (SphK/S1P) are also critical for TGF-beta-stimulated tissue fibrosis; however, it is not clear whether SphK/S1P interacts with miR-21 or not. sphingosine 1-phosphate 46-69 sphingosine kinase 1 Homo sapiens 71-75 26673009-6 2016 In addition, we further evaluated the effect of SphK1 antagonist fingolimod (FTY720) in ACC in vitro and in vivo, as a single agent or in combination with mitotane, and attempted to explore its anticarcinogenic mechanisms. Fingolimod Hydrochloride 65-75 sphingosine kinase 1 Homo sapiens 48-53 26673009-11 2016 SphK1 expression is functionally associated to cellular proliferation, apoptosis, invasion and mitotane sensitivity of ACC. Mitotane 95-103 sphingosine kinase 1 Homo sapiens 0-5 26540633-3 2016 Levels of HULC were positively correlated with levels of SPHK1 and its product, sphingosine-1-phosphate (S1P), in patients HCC samples. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 57-62 26429117-1 2016 Sphingosine kinase (there are two isoforms, SK1 and SK2) catalyses the formation of sphingosine 1-phosphate (S1P), a bioactive lipid that can be released from cells to activate a family of G protein-coupled receptors, termed S1P1-5. sphingosine 1-phosphate 84-107 sphingosine kinase 1 Homo sapiens 44-47 25900155-1 2016 BACKGROUND AND OBJECTIVE: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid, which is generated by activation of sphingosine kinase (SK) 1 and/or 2 in most mammalian cells with various stimuli, including the oral pathogen Aggregatibacter actinomycetemcomitans. sphingosine 1-phosphate 26-49 sphingosine kinase 1 Homo sapiens 121-146 25900155-1 2016 BACKGROUND AND OBJECTIVE: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid, which is generated by activation of sphingosine kinase (SK) 1 and/or 2 in most mammalian cells with various stimuli, including the oral pathogen Aggregatibacter actinomycetemcomitans. Sphingolipids 71-83 sphingosine kinase 1 Homo sapiens 121-146 27148053-6 2016 Importantly, we highlight a profound role of Sphk1 in secretion of inflammatory cytokines and survival of dendritic cells that might be mediated by a change in sphingolipid levels as well as by a change in STAT3 expression. Sphingolipids 160-172 sphingosine kinase 1 Homo sapiens 45-50 27148053-10 2016 Taken together, in this investigation we describe details of a crucial involvement of sphingolipids and Sphk1 in AICD during long-term immunogenic activity of DCs that might play an important role in autoimmunity and might explain the differences in immune response observed in in vivo studies of Sphk1 modulation. Sphingolipids 86-99 sphingosine kinase 1 Homo sapiens 297-302 26429117-9 2016 Indeed, sphingosine, which accumulates upon inhibition of SK1 activity, and sphingosine-like compounds promote activation of the inflammasome, which is linked with multiple sclerosis, to stimulate formation of the pro-inflammatory mediator, IL-1beta. Sphingosine 8-19 sphingosine kinase 1 Homo sapiens 58-61 26667073-4 2016 Sphingosine kinase 1 has emerged as an important determinant of sphingosine-1-phosphate lung levels, which, when aberrantly high, contribute to lung fibrosis, maladaptive vascular remodeling, and allergic asthma. sphingosine 1-phosphate 64-87 sphingosine kinase 1 Homo sapiens 0-20 26293898-7 2016 Further studies showed that WYE-132 inhibited sphingosine kinase-1 (SphK1) activity, leading to pro-apoptotic ceramide production in ovarian cancer cells. WYE 125132 28-35 sphingosine kinase 1 Homo sapiens 46-66 26426296-1 2016 Sphingosine kinase 1 (SphK1) is a lipid kinase that phosphorylates sphingosine to produce the bioactive sphingolipid, sphingosine-1-phosphate (S1P), and therefore represents a potential drug target for a variety of pathological processes such as fibrosis, inflammation, and cancer. Sphingosine 67-78 sphingosine kinase 1 Homo sapiens 22-27 26426296-1 2016 Sphingosine kinase 1 (SphK1) is a lipid kinase that phosphorylates sphingosine to produce the bioactive sphingolipid, sphingosine-1-phosphate (S1P), and therefore represents a potential drug target for a variety of pathological processes such as fibrosis, inflammation, and cancer. Sphingolipids 104-116 sphingosine kinase 1 Homo sapiens 22-27 26426296-1 2016 Sphingosine kinase 1 (SphK1) is a lipid kinase that phosphorylates sphingosine to produce the bioactive sphingolipid, sphingosine-1-phosphate (S1P), and therefore represents a potential drug target for a variety of pathological processes such as fibrosis, inflammation, and cancer. sphingosine 1-phosphate 118-141 sphingosine kinase 1 Homo sapiens 22-27 26426296-3 2016 The biochemical enzyme assay measures the phosphorylation of sphingosine-fluorescein to S1P-fluorescein by recombinant human full-length SphK1 using an immobilized metal affinity for phosphochemicals (IMAP) time-resolved fluorescence resonance energy transfer format. Sphingosine 61-72 sphingosine kinase 1 Homo sapiens 137-142 26426296-3 2016 The biochemical enzyme assay measures the phosphorylation of sphingosine-fluorescein to S1P-fluorescein by recombinant human full-length SphK1 using an immobilized metal affinity for phosphochemicals (IMAP) time-resolved fluorescence resonance energy transfer format. Fluorescein 73-84 sphingosine kinase 1 Homo sapiens 137-142 26426296-3 2016 The biochemical enzyme assay measures the phosphorylation of sphingosine-fluorescein to S1P-fluorescein by recombinant human full-length SphK1 using an immobilized metal affinity for phosphochemicals (IMAP) time-resolved fluorescence resonance energy transfer format. sphingosine 1-phosphate 88-91 sphingosine kinase 1 Homo sapiens 137-142 26426296-3 2016 The biochemical enzyme assay measures the phosphorylation of sphingosine-fluorescein to S1P-fluorescein by recombinant human full-length SphK1 using an immobilized metal affinity for phosphochemicals (IMAP) time-resolved fluorescence resonance energy transfer format. Fluorescein 92-103 sphingosine kinase 1 Homo sapiens 137-142 26426296-3 2016 The biochemical enzyme assay measures the phosphorylation of sphingosine-fluorescein to S1P-fluorescein by recombinant human full-length SphK1 using an immobilized metal affinity for phosphochemicals (IMAP) time-resolved fluorescence resonance energy transfer format. Metals 164-169 sphingosine kinase 1 Homo sapiens 137-142 26426296-4 2016 The yeast assay employs an engineered strain of Saccharomyces cerevisiae, in which the human gene encoding SphK1 replaced the yeast ortholog and quantitates cell viability by measuring intracellular adenosine 5"-triphosphate (ATP) using a luciferase-based luminescent readout. Adenosine Triphosphate 199-224 sphingosine kinase 1 Homo sapiens 107-112 26426296-4 2016 The yeast assay employs an engineered strain of Saccharomyces cerevisiae, in which the human gene encoding SphK1 replaced the yeast ortholog and quantitates cell viability by measuring intracellular adenosine 5"-triphosphate (ATP) using a luciferase-based luminescent readout. Adenosine Triphosphate 226-229 sphingosine kinase 1 Homo sapiens 107-112 26293898-7 2016 Further studies showed that WYE-132 inhibited sphingosine kinase-1 (SphK1) activity, leading to pro-apoptotic ceramide production in ovarian cancer cells. WYE 125132 28-35 sphingosine kinase 1 Homo sapiens 68-73 26293898-7 2016 Further studies showed that WYE-132 inhibited sphingosine kinase-1 (SphK1) activity, leading to pro-apoptotic ceramide production in ovarian cancer cells. Ceramides 110-118 sphingosine kinase 1 Homo sapiens 46-66 26293898-7 2016 Further studies showed that WYE-132 inhibited sphingosine kinase-1 (SphK1) activity, leading to pro-apoptotic ceramide production in ovarian cancer cells. Ceramides 110-118 sphingosine kinase 1 Homo sapiens 68-73 26293898-8 2016 Meanwhile, WYE-132-induced cytotoxicity against ovarian cancer cells was inhibited by sphingosine-1-phosphate (S1P) but was aggravated by SphK1 inhibitor SKI-II or C6 ceramide. WYE 125132 11-18 sphingosine kinase 1 Homo sapiens 138-143 26585812-0 2015 Curcumin Suppresses Phthalate-Induced Metastasis and the Proportion of Cancer Stem Cell (CSC)-like Cells via the Inhibition of AhR/ERK/SK1 Signaling in Hepatocellular Carcinoma. Curcumin 0-8 sphingosine kinase 1 Homo sapiens 135-138 26585812-6 2015 We also reveal that curcumin suppressed phthalate-induced migration, invasion, and CSC-like cell maintenance through inhibition of the aryl hydrocarbon receptor/ERK/SK1/S1P3 signaling pathway. Curcumin 20-28 sphingosine kinase 1 Homo sapiens 165-168 26585812-6 2015 We also reveal that curcumin suppressed phthalate-induced migration, invasion, and CSC-like cell maintenance through inhibition of the aryl hydrocarbon receptor/ERK/SK1/S1P3 signaling pathway. phthalic acid 40-49 sphingosine kinase 1 Homo sapiens 165-168 26246404-2 2015 IL-1 stimulates expression of many chemokines, including C-C motif ligand (CCL) 5, that recruit immune cells, but it also stimulates sphingosine kinase-1, an enzyme that generates sphingosine-1-phosphate (S1P), a bioactive lipid mediator essential for inflammation. sphingosine 1-phosphate 180-203 sphingosine kinase 1 Homo sapiens 133-153 26549227-2 2015 Sphingosine kinase 1 (SPHK1) and its metabolite sphingosine 1-phosphate (S1P) contribute to tumor angiogenesis. sphingosine 1-phosphate 48-71 sphingosine kinase 1 Homo sapiens 0-20 26549227-2 2015 Sphingosine kinase 1 (SPHK1) and its metabolite sphingosine 1-phosphate (S1P) contribute to tumor angiogenesis. sphingosine 1-phosphate 48-71 sphingosine kinase 1 Homo sapiens 22-27 25892494-2 2015 Sphingosine kinase 1 (SK1) is an oncogenic protein that converts sphingosine to sphingosine 1-phosphate (S1P), which is a messenger molecule involved in calcium signaling. Calcium 153-160 sphingosine kinase 1 Homo sapiens 22-25 26271487-1 2015 UNLABELLED: The bioactive sphingolipid sphingosine-1-phosphate (S1P) drives several hallmark processes of cancer, making the enzymes that synthesize S1P, that is, sphingosine kinase 1 and 2 (SK1 and SK2), important molecular targets for cancer drug development. Sphingolipids 26-38 sphingosine kinase 1 Homo sapiens 163-189 26271487-1 2015 UNLABELLED: The bioactive sphingolipid sphingosine-1-phosphate (S1P) drives several hallmark processes of cancer, making the enzymes that synthesize S1P, that is, sphingosine kinase 1 and 2 (SK1 and SK2), important molecular targets for cancer drug development. Sphingolipids 26-38 sphingosine kinase 1 Homo sapiens 191-194 26271487-1 2015 UNLABELLED: The bioactive sphingolipid sphingosine-1-phosphate (S1P) drives several hallmark processes of cancer, making the enzymes that synthesize S1P, that is, sphingosine kinase 1 and 2 (SK1 and SK2), important molecular targets for cancer drug development. sphingosine 1-phosphate 39-62 sphingosine kinase 1 Homo sapiens 163-189 26271487-1 2015 UNLABELLED: The bioactive sphingolipid sphingosine-1-phosphate (S1P) drives several hallmark processes of cancer, making the enzymes that synthesize S1P, that is, sphingosine kinase 1 and 2 (SK1 and SK2), important molecular targets for cancer drug development. sphingosine 1-phosphate 39-62 sphingosine kinase 1 Homo sapiens 191-194 26476183-3 2015 In this study we report that treatment of endothelial cells (ECs) with 17beta-estradiol (E2) resulted in a rapid, transient, and dose-dependent increase in SphK activity and increased S1P production. Estradiol 71-87 sphingosine kinase 1 Homo sapiens 156-160 26590717-4 2015 Mechanistically, this is achieved by direct association of Khps1 with a homopurine stretch upstream of the transcription start site of SPHK1, which forms a DNA-RNA triplex that anchors the lncRNA and associated effector proteins to the gene promoter. homopurine 72-82 sphingosine kinase 1 Homo sapiens 135-140 26742972-7 2015 CONCLUSIONS: SPK1 is expressed in the human bladder wall, specifically the urothelium, in bladder specimens from patients with NDO. NHDP 127-130 sphingosine kinase 1 Homo sapiens 13-17 26493335-0 2015 Regulation of cellular sphingosine-1-phosphate by sphingosine kinase 1 and sphingosine-1-phopshate lyase determines chemotherapy resistance in gastroesophageal cancer. sphingosine 1-phosphate 23-46 sphingosine kinase 1 Homo sapiens 50-104 26493335-12 2015 SPHK1:SGPL1 ratio correlated with increased cellular sphingosine-1-phosphate (S1P), and S1P correlated with drug resistance (IC50). sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 0-5 26493335-13 2015 High SPHK1 protein correlated with resistance to cisplatin (IC50) in an independent gastric cancer cell line panel and with survival of patients treated with chemotherapy prior to surgery but not in patients treated with surgery alone. Cisplatin 49-58 sphingosine kinase 1 Homo sapiens 5-10 26493335-14 2015 Safingol a SPHK1 inhibitor, was cytotoxic as a single agent and acted synergistically with cisplatin in gastric cancer cell lines. safingol 0-8 sphingosine kinase 1 Homo sapiens 11-16 26311741-3 2015 SPHK1 expression was examined in 287 formalin-fixed, paraffin-embedded cervical cancer tissues using immunohistochemistry, and its clinical implications and prognostic significance were analyzed. Formaldehyde 37-45 sphingosine kinase 1 Homo sapiens 0-5 26311741-3 2015 SPHK1 expression was examined in 287 formalin-fixed, paraffin-embedded cervical cancer tissues using immunohistochemistry, and its clinical implications and prognostic significance were analyzed. Paraffin 53-61 sphingosine kinase 1 Homo sapiens 0-5 26090720-2 2015 After analyzing the gene expression profiles of seven paired intrahepatic CCA microarrays, a novel sphingosine kinase 1 (SPHK1)/sphingosine-1-phosphate (S1P) pathway and a novel target gene, SPHK1, were identified. sphingosine 1-phosphate 128-151 sphingosine kinase 1 Homo sapiens 99-119 26090720-2 2015 After analyzing the gene expression profiles of seven paired intrahepatic CCA microarrays, a novel sphingosine kinase 1 (SPHK1)/sphingosine-1-phosphate (S1P) pathway and a novel target gene, SPHK1, were identified. sphingosine 1-phosphate 128-151 sphingosine kinase 1 Homo sapiens 121-126 26090720-6 2015 SK1-I modulated the balance of ceramide-sphinogosine-S1P and induced CCA apoptosis. Ceramides 31-39 sphingosine kinase 1 Homo sapiens 0-3 26090720-6 2015 SK1-I modulated the balance of ceramide-sphinogosine-S1P and induced CCA apoptosis. sphinogosine-s1p 40-56 sphingosine kinase 1 Homo sapiens 0-3 26337959-7 2015 While C6 ceramide and SphK1 inhibitor SKI-II facilitated ABC294640-induced cytotoxicity against HT-29 cells. 3-(4-chlorophenyl)-adamantane-1-carboxylic acid (pyridin-4-ylmethyl)amide 57-66 sphingosine kinase 1 Homo sapiens 22-27 25892494-2 2015 Sphingosine kinase 1 (SK1) is an oncogenic protein that converts sphingosine to sphingosine 1-phosphate (S1P), which is a messenger molecule involved in calcium signaling. Sphingosine 65-76 sphingosine kinase 1 Homo sapiens 0-20 25892494-2 2015 Sphingosine kinase 1 (SK1) is an oncogenic protein that converts sphingosine to sphingosine 1-phosphate (S1P), which is a messenger molecule involved in calcium signaling. Sphingosine 65-76 sphingosine kinase 1 Homo sapiens 22-25 25892494-2 2015 Sphingosine kinase 1 (SK1) is an oncogenic protein that converts sphingosine to sphingosine 1-phosphate (S1P), which is a messenger molecule involved in calcium signaling. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 0-20 25892494-2 2015 Sphingosine kinase 1 (SK1) is an oncogenic protein that converts sphingosine to sphingosine 1-phosphate (S1P), which is a messenger molecule involved in calcium signaling. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 22-25 25892494-2 2015 Sphingosine kinase 1 (SK1) is an oncogenic protein that converts sphingosine to sphingosine 1-phosphate (S1P), which is a messenger molecule involved in calcium signaling. Calcium 153-160 sphingosine kinase 1 Homo sapiens 0-20 26113114-0 2015 Sphingosine kinase 1 activation enhances epidermal innate immunity through sphingosine-1-phosphate stimulation of cathelicidin production. sphingosine 1-phosphate 75-98 sphingosine kinase 1 Homo sapiens 0-20 26113114-3 2015 OBJECTIVE: We investigated whether pharmacological modulation of SPHK1 activity, using a novel synthetic SPHK1 activator, (S)-methyl 2-(hexanamide)-3-(4-hydroxyphenyl) propanoate (MHP), stimulates CAMP expression. methyl 2-(hexanamide)-3-(4-hydroxyphenyl)propanoate 122-178 sphingosine kinase 1 Homo sapiens 65-70 26113114-3 2015 OBJECTIVE: We investigated whether pharmacological modulation of SPHK1 activity, using a novel synthetic SPHK1 activator, (S)-methyl 2-(hexanamide)-3-(4-hydroxyphenyl) propanoate (MHP), stimulates CAMP expression. methyl 2-(hexanamide)-3-(4-hydroxyphenyl)propanoate 122-178 sphingosine kinase 1 Homo sapiens 105-110 26113114-7 2015 Next, we found that exogenous MHP significantly stimulated CAMP mRNA and protein production in KC, increases that were significantly suppressed by siRNA directed against SPHK1, but not by a scrambled control siRNA. methyl 2-(hexanamide)-3-(4-hydroxyphenyl)propanoate 30-33 sphingosine kinase 1 Homo sapiens 170-175 26113114-9 2015 Conversely, pretreatment with a specific inhibitor of SPHK1 decreased MHP-induced nuclear translocation of NF-kappaB, and significantly attenuated the MHP-mediated increase in CAMP production. methyl 2-(hexanamide)-3-(4-hydroxyphenyl)propanoate 70-73 sphingosine kinase 1 Homo sapiens 54-59 25869501-12 2015 Our results suggest the pro-migratory role of CRFR1-Sphk1-S1P-S1PR3-cPLA2 signaling pathway in VSMCs. vsmcs 95-100 sphingosine kinase 1 Homo sapiens 52-57 26183376-5 2015 Contrarily, synergistic gene induction of sphingosine kinase 1 (SphK1) by glucocorticoids and pro-inflammatory stimuli via the glucocorticoid receptor in macrophages increases circulating sphingosine 1-phosphate levels, which proves essential for the inhibition of inflammation. sphingosine 1-phosphate 188-211 sphingosine kinase 1 Homo sapiens 42-62 26183376-5 2015 Contrarily, synergistic gene induction of sphingosine kinase 1 (SphK1) by glucocorticoids and pro-inflammatory stimuli via the glucocorticoid receptor in macrophages increases circulating sphingosine 1-phosphate levels, which proves essential for the inhibition of inflammation. sphingosine 1-phosphate 188-211 sphingosine kinase 1 Homo sapiens 64-69 25769964-5 2015 Tamoxifen also depressed expression and activity of sphingosine kinase 1 (SphK1), the enzyme-catalyzing production of mitogenic sphingosine 1-phosphate (S1-P). Tamoxifen 0-9 sphingosine kinase 1 Homo sapiens 52-72 25769964-5 2015 Tamoxifen also depressed expression and activity of sphingosine kinase 1 (SphK1), the enzyme-catalyzing production of mitogenic sphingosine 1-phosphate (S1-P). Tamoxifen 0-9 sphingosine kinase 1 Homo sapiens 74-79 25769964-5 2015 Tamoxifen also depressed expression and activity of sphingosine kinase 1 (SphK1), the enzyme-catalyzing production of mitogenic sphingosine 1-phosphate (S1-P). sphingosine 1-phosphate 128-151 sphingosine kinase 1 Homo sapiens 52-72 25769964-5 2015 Tamoxifen also depressed expression and activity of sphingosine kinase 1 (SphK1), the enzyme-catalyzing production of mitogenic sphingosine 1-phosphate (S1-P). sphingosine 1-phosphate 128-151 sphingosine kinase 1 Homo sapiens 74-79 26807182-8 2015 Overexpression of miR-125b led to the downregulation of SphK1 and protein level as assessed by qRT-PCR and Western blot. mir-125b 18-26 sphingosine kinase 1 Homo sapiens 56-61 25862200-1 2015 Sphingosine-1-phosphate (S1P) is a ubiquitous, endogenous small molecule that is synthesized by two isoforms of sphingosine kinase (SphK1 and 2). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 132-143 26264277-3 2015 Sphingosine kinase 1 (SphK1), a negative regulator of ceramide accumulation with antiapoptotic effects, was markedly elevated in multiple myeloma cells. Ceramides 54-62 sphingosine kinase 1 Homo sapiens 0-20 26264277-3 2015 Sphingosine kinase 1 (SphK1), a negative regulator of ceramide accumulation with antiapoptotic effects, was markedly elevated in multiple myeloma cells. Ceramides 54-62 sphingosine kinase 1 Homo sapiens 22-27 26264277-4 2015 The silencing of SphK1 potentiated the apoptotic effects of the green tea polyphenol epigallocatechin-3-O-gallate (EGCG), an activator of ASM through 67LR. polyphenol epigallocatechin-3-o-gallate 74-113 sphingosine kinase 1 Homo sapiens 17-22 26264277-4 2015 The silencing of SphK1 potentiated the apoptotic effects of the green tea polyphenol epigallocatechin-3-O-gallate (EGCG), an activator of ASM through 67LR. epigallocatechin gallate 115-119 sphingosine kinase 1 Homo sapiens 17-22 26264277-5 2015 Furthermore, the SphK1 inhibitor safingol synergistically sensitized EGCG-induced proapoptotic cell death and tumor suppression in multiple myeloma cells by promoting the prevention of RTK phosphorylation and activation of death-associated protein kinase 1 (DAPK1). safingol 33-41 sphingosine kinase 1 Homo sapiens 17-22 26264277-5 2015 Furthermore, the SphK1 inhibitor safingol synergistically sensitized EGCG-induced proapoptotic cell death and tumor suppression in multiple myeloma cells by promoting the prevention of RTK phosphorylation and activation of death-associated protein kinase 1 (DAPK1). epigallocatechin gallate 69-73 sphingosine kinase 1 Homo sapiens 17-22 26071354-4 2015 At the molecular level, miR-101 dramatically down-regulated SphK1 mRNA and protein expression, causing pro-apoptotic ceramide production in above CRC cells. Ceramides 117-125 sphingosine kinase 1 Homo sapiens 60-65 26071354-5 2015 On the other hand, inhibition of miR-101 through expressing antagomiR-101 increased SphK1 expression to down-regulate ceramide level in HT-29 cells. Ceramides 118-126 sphingosine kinase 1 Homo sapiens 84-89 26071354-7 2015 CRC cells with SphK1-shRNA knockdown showed similar phenotypes as the miR-101-expressed CRC cells, presenting with elevated level of ceramide and high sensitivity to paclitaxel or doxorubicin. Ceramides 133-141 sphingosine kinase 1 Homo sapiens 15-20 26071354-7 2015 CRC cells with SphK1-shRNA knockdown showed similar phenotypes as the miR-101-expressed CRC cells, presenting with elevated level of ceramide and high sensitivity to paclitaxel or doxorubicin. Paclitaxel 166-176 sphingosine kinase 1 Homo sapiens 15-20 26071354-7 2015 CRC cells with SphK1-shRNA knockdown showed similar phenotypes as the miR-101-expressed CRC cells, presenting with elevated level of ceramide and high sensitivity to paclitaxel or doxorubicin. Doxorubicin 180-191 sphingosine kinase 1 Homo sapiens 15-20 25724183-4 2015 Further, cinobufotalin inhibited sphingosine kinase 1 (SphK1) activity and induced pro-apoptotic ceramide production. cinobufotalin 9-22 sphingosine kinase 1 Homo sapiens 33-53 25724183-4 2015 Further, cinobufotalin inhibited sphingosine kinase 1 (SphK1) activity and induced pro-apoptotic ceramide production. cinobufotalin 9-22 sphingosine kinase 1 Homo sapiens 55-60 25724183-7 2015 SphK1 inhibitor II (SKI-II), similar to cinobufotalin, increased cellular ceramide level and promoted HCC cell apoptosis. Ceramides 74-82 sphingosine kinase 1 Homo sapiens 0-5 26193362-6 2015 We found that BCPs with low percentage of HA favored the expression of positive coupling factors, including sphingosine-kinase 1 (SPHK1) and collagen triple helix repeat containing 1 (Cthrc1). hydroxyapatite-beta tricalcium phosphate 14-18 sphingosine kinase 1 Homo sapiens 108-128 26193362-6 2015 We found that BCPs with low percentage of HA favored the expression of positive coupling factors, including sphingosine-kinase 1 (SPHK1) and collagen triple helix repeat containing 1 (Cthrc1). hydroxyapatite-beta tricalcium phosphate 14-18 sphingosine kinase 1 Homo sapiens 130-135 25805832-1 2015 Sphingosine kinase 1 (SK1), the enzyme responsible for sphingosine 1-phosphate (S1P) production, is overexpressed in many human solid tumors. sphingosine 1-phosphate 55-78 sphingosine kinase 1 Homo sapiens 0-20 25805832-1 2015 Sphingosine kinase 1 (SK1), the enzyme responsible for sphingosine 1-phosphate (S1P) production, is overexpressed in many human solid tumors. sphingosine 1-phosphate 55-78 sphingosine kinase 1 Homo sapiens 22-25 25805832-1 2015 Sphingosine kinase 1 (SK1), the enzyme responsible for sphingosine 1-phosphate (S1P) production, is overexpressed in many human solid tumors. sphingosine 1-phosphate 80-83 sphingosine kinase 1 Homo sapiens 0-20 25805832-1 2015 Sphingosine kinase 1 (SK1), the enzyme responsible for sphingosine 1-phosphate (S1P) production, is overexpressed in many human solid tumors. sphingosine 1-phosphate 80-83 sphingosine kinase 1 Homo sapiens 22-25 25892494-7 2015 HeLa cells overexpressing SK1 showed increased [Ca2+]cav during histamine-induced Ca2+ mobilization in the absence of extracellular Ca2+ as well as during receptor-operated Ca2+ entry (ROCE). Histamine 64-73 sphingosine kinase 1 Homo sapiens 26-29 25587035-4 2015 Here we report that adenosine induces SphK1 activity in human and mouse sickle and normal erythrocytes in vitro. Adenosine 20-29 sphingosine kinase 1 Homo sapiens 38-43 25942476-8 2015 In addition, a marked reduction in sphingosine kinase 1 (SPHK1) expression/activity due to impaired TGFbeta signalling via ALK1/SMAD1 contributed to the sphingosine buildup in IUGR. Sphingosine 35-46 sphingosine kinase 1 Homo sapiens 57-62 26198952-0 2015 [Sphingosine kinase 1 promotes glioma cell proliferation under hypoxia via calcium signaling]. Calcium 75-82 sphingosine kinase 1 Homo sapiens 1-21 26198952-3 2015 The cells transfected with SphK1 siRNA and with a negative control siRNA were then exposed to 3% oxygen or 150 micromol/L CoCl2 to induce hypoxia. Oxygen 97-103 sphingosine kinase 1 Homo sapiens 27-32 26198952-5 2015 RESULTS: SphK1 knockdown significantly reduced hypoxia-induced calcium reflux and suppressed the cell proliferation. Calcium 63-70 sphingosine kinase 1 Homo sapiens 9-14 26198952-7 2015 CONCLUSION: SphK1 promotes the proliferation of glioma cells under hypoxia via regulating calcium signaling. Calcium 90-97 sphingosine kinase 1 Homo sapiens 12-17 25788259-4 2015 Here we report a first-in-class ATP-binding site-directed small molecule SK inhibitor, MP-A08, discovered using an approach of structural homology modelling of the ATP-binding site of SK1 and in silico docking with small molecule libraries. Adenosine Triphosphate 32-35 sphingosine kinase 1 Homo sapiens 184-187 25788259-4 2015 Here we report a first-in-class ATP-binding site-directed small molecule SK inhibitor, MP-A08, discovered using an approach of structural homology modelling of the ATP-binding site of SK1 and in silico docking with small molecule libraries. MP-A08 87-93 sphingosine kinase 1 Homo sapiens 184-187 25788259-4 2015 Here we report a first-in-class ATP-binding site-directed small molecule SK inhibitor, MP-A08, discovered using an approach of structural homology modelling of the ATP-binding site of SK1 and in silico docking with small molecule libraries. Adenosine Triphosphate 164-167 sphingosine kinase 1 Homo sapiens 184-187 25788259-5 2015 MP-A08 is a highly selective ATP competitive SK inhibitor that targets both SK1 and SK2. MP-A08 0-6 sphingosine kinase 1 Homo sapiens 76-79 25788259-5 2015 MP-A08 is a highly selective ATP competitive SK inhibitor that targets both SK1 and SK2. Adenosine Triphosphate 29-32 sphingosine kinase 1 Homo sapiens 76-79 25563902-1 2015 We previously developed SKI-178 (N"-[(1E)-1-(3,4-dimethoxyphenyl)ethylidene]-3-(4-methoxxyphenyl)-1H-pyrazole-5-carbohydrazide) as a novel sphingosine kinase-1 (SphK1) selective inhibitor and, herein, sought to determine the mechanism-of-action of SKI-178-induced cell death. n"-[(1e)-1-(3,4-dimethoxyphenyl)ethylidene]-3-(4-methoxxyphenyl)-1h-pyrazole-5-carbohydrazide 33-126 sphingosine kinase 1 Homo sapiens 139-159 25778867-5 2015 Immunohistochemistry (IHC) was conducted to characterize the expression pattern of SPHK1 in 142 archived paraffin-embedded NPC specimens. Paraffin 105-113 sphingosine kinase 1 Homo sapiens 83-88 26045781-3 2015 Western blotting was performed for detecting changes of SphK1 expression in total cell protein and membrane/cytosol protein in K562 cells respectively after exposure to resveratrol. Resveratrol 169-180 sphingosine kinase 1 Homo sapiens 56-61 26045781-4 2015 A biochemical assay was used to measure the activity of SphK after treatment of resveratrol, and then S1P and ceramide levels were examined using ELISA kits. Resveratrol 80-91 sphingosine kinase 1 Homo sapiens 56-60 26045781-7 2015 Western blotting revealed that resveratrol did not affect total SphK1 expression level in K562 cells, but significantly changed the translocation of SphK1, the membrane SphK1 was decreased while cytosol SphK1 level was elevated. Resveratrol 31-42 sphingosine kinase 1 Homo sapiens 149-154 26045781-7 2015 Western blotting revealed that resveratrol did not affect total SphK1 expression level in K562 cells, but significantly changed the translocation of SphK1, the membrane SphK1 was decreased while cytosol SphK1 level was elevated. Resveratrol 31-42 sphingosine kinase 1 Homo sapiens 149-154 26045781-7 2015 Western blotting revealed that resveratrol did not affect total SphK1 expression level in K562 cells, but significantly changed the translocation of SphK1, the membrane SphK1 was decreased while cytosol SphK1 level was elevated. Resveratrol 31-42 sphingosine kinase 1 Homo sapiens 149-154 26045781-8 2015 The activity of SphK1 in resveratrol treated groups was decreased compared to control group with a significant decrease of S1P and increase of ceramide level. Resveratrol 25-36 sphingosine kinase 1 Homo sapiens 16-21 26045781-8 2015 The activity of SphK1 in resveratrol treated groups was decreased compared to control group with a significant decrease of S1P and increase of ceramide level. Ceramides 143-151 sphingosine kinase 1 Homo sapiens 16-21 26045781-10 2015 Resveratrol-induced proliferation inhibition of K562 cells might be mediated through its modulation activity of SphK1 pathway by regulating S1P and ceramide levels, which then affected the proliferation and apoptosis process of leukemia cells. Resveratrol 0-11 sphingosine kinase 1 Homo sapiens 112-117 26045781-10 2015 Resveratrol-induced proliferation inhibition of K562 cells might be mediated through its modulation activity of SphK1 pathway by regulating S1P and ceramide levels, which then affected the proliferation and apoptosis process of leukemia cells. Ceramides 148-156 sphingosine kinase 1 Homo sapiens 112-117 26045781-11 2015 SphK1/S1P pathway represents a target of resveratrol in human leukemia. Resveratrol 41-52 sphingosine kinase 1 Homo sapiens 0-5 25563902-1 2015 We previously developed SKI-178 (N"-[(1E)-1-(3,4-dimethoxyphenyl)ethylidene]-3-(4-methoxxyphenyl)-1H-pyrazole-5-carbohydrazide) as a novel sphingosine kinase-1 (SphK1) selective inhibitor and, herein, sought to determine the mechanism-of-action of SKI-178-induced cell death. n"-[(1e)-1-(3,4-dimethoxyphenyl)ethylidene]-3-(4-methoxxyphenyl)-1h-pyrazole-5-carbohydrazide 33-126 sphingosine kinase 1 Homo sapiens 161-166 25537088-10 2015 DMS inhibited the proliferation of SK-Hep1 and MHCCLM3 cells which have a relatively high level of SphK1 in a time- and concentration-dependent manner, and the invasion and migration of SK-Hep1 cells were distinctly suppressed after undergoing treatment with DMS. N,N-dimethylsphingosine 0-3 sphingosine kinase 1 Homo sapiens 99-104 25537088-3 2015 The purpose of this study was to determine the correlation between HCC and SphK1, and to evaluate the effect of SphK1 inhibitor N,N-dimethylsphingosine (DMS) in HCC. N,N-dimethylsphingosine 128-151 sphingosine kinase 1 Homo sapiens 112-117 25643074-4 2015 Herein, we report the design, synthesis, and structure-activity relationship studies of guanidine-based SphK inhibitors bearing an oxadiazole ring in the scaffold. Oxadiazoles 131-141 sphingosine kinase 1 Homo sapiens 104-108 25643074-4 2015 Herein, we report the design, synthesis, and structure-activity relationship studies of guanidine-based SphK inhibitors bearing an oxadiazole ring in the scaffold. Guanidine 88-97 sphingosine kinase 1 Homo sapiens 104-108 25643074-6 2015 Surprisingly, homologation with a single methylene unit between the oxadiazole and heterocyclic ring afforded a SphK1-selective inhibitor in SLP7111228 (Ki = 48 nM), which also decreased S1P levels in cultured U937 cells. Oxadiazoles 68-78 sphingosine kinase 1 Homo sapiens 112-117 25654428-5 2015 In our docking model, pachastrissamine and 4b were able to effectively bind to the binding pocket of sphingosine kinase 1 as co-crystalized sphingosine. pachastrissamine 22-38 sphingosine kinase 1 Homo sapiens 101-121 25505239-5 2015 Perturbation of the sphingolipid pathway by disruption of the sphingosine kinase gene (Sphk) during pregnancy caused unusually high expression of neutrophil chemoattractants, CXCL1 and CXCL2, in the decidua, leading to a massive infiltration of neutrophils into the fetomaternal interface with enhanced oxidative damage, resulting in early fetal death. Sphingolipids 20-32 sphingosine kinase 1 Homo sapiens 62-85 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. gamma phosphate 20-35 sphingosine kinase 1 Homo sapiens 153-172 25505239-5 2015 Perturbation of the sphingolipid pathway by disruption of the sphingosine kinase gene (Sphk) during pregnancy caused unusually high expression of neutrophil chemoattractants, CXCL1 and CXCL2, in the decidua, leading to a massive infiltration of neutrophils into the fetomaternal interface with enhanced oxidative damage, resulting in early fetal death. Sphingolipids 20-32 sphingosine kinase 1 Homo sapiens 87-91 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. gamma phosphate 20-35 sphingosine kinase 1 Homo sapiens 174-178 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. gamma phosphate 20-35 sphingosine kinase 1 Homo sapiens 210-215 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Adenosine Triphosphate 41-44 sphingosine kinase 1 Homo sapiens 153-172 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Adenosine Triphosphate 41-44 sphingosine kinase 1 Homo sapiens 174-178 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Adenosine Triphosphate 41-44 sphingosine kinase 1 Homo sapiens 210-215 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Sphingosine 48-59 sphingosine kinase 1 Homo sapiens 153-172 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Sphingosine 48-59 sphingosine kinase 1 Homo sapiens 174-178 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Sphingosine 48-59 sphingosine kinase 1 Homo sapiens 210-215 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Sphingosine 61-64 sphingosine kinase 1 Homo sapiens 153-172 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Sphingosine 61-64 sphingosine kinase 1 Homo sapiens 174-178 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. Sphingosine 61-64 sphingosine kinase 1 Homo sapiens 210-215 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. sphingosine 1-phosphate 106-129 sphingosine kinase 1 Homo sapiens 153-172 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. sphingosine 1-phosphate 106-129 sphingosine kinase 1 Homo sapiens 174-178 25384187-1 2015 The transfer of the gamma phosphate from ATP to sphingosine (Sph) to generate a small signaling molecule, sphingosine 1-phosphate (S1P), is catalyzed by sphingosine kinases (SphK), which exist as two isoforms, SphK1 and SphK2. sphingosine 1-phosphate 106-129 sphingosine kinase 1 Homo sapiens 210-215 25384187-2 2015 SphK is a key regulator of S1P and the S1P:Sph/ceramide ratio. Ceramides 47-55 sphingosine kinase 1 Homo sapiens 0-4 25446881-10 2015 Administration of SphK1 inhibitor 8 days post-bleomycin challenge reduced bleomycin-induced mortality and pulmonary fibrosis. Bleomycin 46-55 sphingosine kinase 1 Homo sapiens 18-23 25411162-0 2015 A novel E2F/sphingosine kinase 1 axis regulates anthracycline response in squamous cell carcinoma. Anthracyclines 48-61 sphingosine kinase 1 Homo sapiens 12-32 25411162-11 2015 Moreover, inhibition of Sphk1 by shRNA or the Sphk1-specific inhibitor, SK1-I (BML-EI411), enhanced the sensitivity of SCC cells to doxorubicin in vitro and in vivo. Doxorubicin 132-143 sphingosine kinase 1 Homo sapiens 24-29 25411162-11 2015 Moreover, inhibition of Sphk1 by shRNA or the Sphk1-specific inhibitor, SK1-I (BML-EI411), enhanced the sensitivity of SCC cells to doxorubicin in vitro and in vivo. Doxorubicin 132-143 sphingosine kinase 1 Homo sapiens 46-51 25411162-12 2015 Furthermore, E2F7-induced doxorubicin resistance was mediated via Sphk1-dependent activation of AKT in vitro and in vivo. Doxorubicin 26-37 sphingosine kinase 1 Homo sapiens 66-71 25446881-10 2015 Administration of SphK1 inhibitor 8 days post-bleomycin challenge reduced bleomycin-induced mortality and pulmonary fibrosis. Bleomycin 74-83 sphingosine kinase 1 Homo sapiens 18-23 25176316-7 2015 The angiogenic response to oxLDL was blocked by the sphingosine kinase (SPHK) inhibitor, dimethylsphingosine, by SPHK1-siRNA and by an anti-S1P monoclonal antibody. dimethylsphingosine 89-108 sphingosine kinase 1 Homo sapiens 52-70 25446881-6 2015 Further, the expressions of SphK1 and S1PL were increased in lung tissues from patients with IPF and bleomycin-challenged mice. Bleomycin 101-110 sphingosine kinase 1 Homo sapiens 28-33 26252351-2 2015 Sphingosine-1-phosphate (S1P) is a potent bioactive sphingolipid metabolite produced by sphingosine kinases-1 and -2 (SPHK1 and SPHK2). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 88-116 26252351-2 2015 Sphingosine-1-phosphate (S1P) is a potent bioactive sphingolipid metabolite produced by sphingosine kinases-1 and -2 (SPHK1 and SPHK2). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 118-123 26252351-2 2015 Sphingosine-1-phosphate (S1P) is a potent bioactive sphingolipid metabolite produced by sphingosine kinases-1 and -2 (SPHK1 and SPHK2). sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 88-116 26252351-2 2015 Sphingosine-1-phosphate (S1P) is a potent bioactive sphingolipid metabolite produced by sphingosine kinases-1 and -2 (SPHK1 and SPHK2). sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 118-123 26252351-2 2015 Sphingosine-1-phosphate (S1P) is a potent bioactive sphingolipid metabolite produced by sphingosine kinases-1 and -2 (SPHK1 and SPHK2). Sphingolipids 52-64 sphingosine kinase 1 Homo sapiens 88-116 26252351-2 2015 Sphingosine-1-phosphate (S1P) is a potent bioactive sphingolipid metabolite produced by sphingosine kinases-1 and -2 (SPHK1 and SPHK2). Sphingolipids 52-64 sphingosine kinase 1 Homo sapiens 118-123 25176316-7 2015 The angiogenic response to oxLDL was blocked by the sphingosine kinase (SPHK) inhibitor, dimethylsphingosine, by SPHK1-siRNA and by an anti-S1P monoclonal antibody. dimethylsphingosine 89-108 sphingosine kinase 1 Homo sapiens 72-76 25176316-8 2015 Moreover, inhibition of oxLDL uptake and subsequent redox signalling by anti-CD36 and anti-LOX-1 receptor antibodies and by N-acetylcysteine, respectively, blocked SPHK1 activation and tube formation. Acetylcysteine 124-140 sphingosine kinase 1 Homo sapiens 164-169 25417698-3 2014 Sphingosine accumulation due to inactivation of VEGF/SphK pathway led to PNs loss via inhibition of autophagosome-lysosome fusion in NP-C mice. Sphingosine 0-11 sphingosine kinase 1 Homo sapiens 53-57 25723458-1 2015 Sphingosine kinases (Sphk1 and 2) regulate the prodution of sphingosine-1-phosphate (S1P), that is key molecule in cancer development. sphingosine 1-phosphate 60-83 sphingosine kinase 1 Homo sapiens 21-32 25723458-4 2015 Different growth factorsinduced activation of receptor tyrosine kinases (RTKs) results in production of Sphk1 which catalyzes the phosphorylation of sphingosine. Sphingosine 149-160 sphingosine kinase 1 Homo sapiens 104-109 24276247-1 2014 Sphingosine kinase 1 (SK1) is a lipid kinase that catalyses the formation of sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 0-20 24276247-1 2014 Sphingosine kinase 1 (SK1) is a lipid kinase that catalyses the formation of sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 22-25 25417698-4 2014 VEGF activates SphK by binding to VEGFR2, resulting in decreased sphingosine storage as well as improved PNs survival and clinical outcomes in NP-C cells and mice. Sphingosine 65-76 sphingosine kinase 1 Homo sapiens 15-19 25216046-1 2014 Sphingosine kinase 1 (SK1) is a signaling enzyme that catalyzes the formation of sphingosine-1-phosphate. sphingosine 1-phosphate 81-104 sphingosine kinase 1 Homo sapiens 0-20 25216046-1 2014 Sphingosine kinase 1 (SK1) is a signaling enzyme that catalyzes the formation of sphingosine-1-phosphate. sphingosine 1-phosphate 81-104 sphingosine kinase 1 Homo sapiens 22-25 25197261-0 2014 Sphingosine kinase 1 mediates head & neck squamous cell carcinoma invasion through sphingosine 1-phosphate receptor 1. Adenosine Monophosphate 36-39 sphingosine kinase 1 Homo sapiens 0-20 25516793-2 2014 The crystal structure of SPHK1 in complex with inhibitor PF-543 to 1.8 A resolution reveals the inhibitor bound in a bent conformation analogous to that expected of a bound sphingosine substrate but with a rotated head group. Sphingosine 173-184 sphingosine kinase 1 Homo sapiens 25-30 26842184-4 2014 The effect of tumor necrosis factor alpha (TNF-alpha) and/or N, N-dimethylsphingosine (DMS) on the Sphk1 and E-cad expression, MCF-10A cell proliferation and invasion was investigated. N,N-dimethylsphingosine 61-85 sphingosine kinase 1 Homo sapiens 99-104 26842184-4 2014 The effect of tumor necrosis factor alpha (TNF-alpha) and/or N, N-dimethylsphingosine (DMS) on the Sphk1 and E-cad expression, MCF-10A cell proliferation and invasion was investigated. dms 87-90 sphingosine kinase 1 Homo sapiens 99-104 25109763-8 2014 In addition, the combination of FTY720 and cisplatin reduced the protein expression of SK1 and the phosphorylation levels of phosphoinositide 3-kinase (PI3K), Akt and mTOR in the SK-Mel-28 cells; the expression of epidermal growth factor receptor (EGFR) was also markedly reduced. Fingolimod Hydrochloride 32-38 sphingosine kinase 1 Homo sapiens 87-90 25109763-8 2014 In addition, the combination of FTY720 and cisplatin reduced the protein expression of SK1 and the phosphorylation levels of phosphoinositide 3-kinase (PI3K), Akt and mTOR in the SK-Mel-28 cells; the expression of epidermal growth factor receptor (EGFR) was also markedly reduced. Cisplatin 43-52 sphingosine kinase 1 Homo sapiens 87-90 24471412-1 2014 Sphingosine kinase (SphK) is an oncogenic lipid kinase that regulates the sphingolipid metabolic pathway that has been shown to play a role in numerous hyperproliferative/inflammatory diseases. Sphingolipids 74-86 sphingosine kinase 1 Homo sapiens 0-18 24471412-1 2014 Sphingosine kinase (SphK) is an oncogenic lipid kinase that regulates the sphingolipid metabolic pathway that has been shown to play a role in numerous hyperproliferative/inflammatory diseases. Sphingolipids 74-86 sphingosine kinase 1 Homo sapiens 20-24 24471412-2 2014 The SphK isoforms (SphK1 and SphK2) catalyze the conversion of the proapoptotic substrate d-erythrosphingosine to the promitogenic/migratory product sphingosine 1-phosphate (S1P). Sphingosine 90-110 sphingosine kinase 1 Homo sapiens 4-8 24471412-2 2014 The SphK isoforms (SphK1 and SphK2) catalyze the conversion of the proapoptotic substrate d-erythrosphingosine to the promitogenic/migratory product sphingosine 1-phosphate (S1P). Sphingosine 90-110 sphingosine kinase 1 Homo sapiens 19-24 24471412-2 2014 The SphK isoforms (SphK1 and SphK2) catalyze the conversion of the proapoptotic substrate d-erythrosphingosine to the promitogenic/migratory product sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 149-172 sphingosine kinase 1 Homo sapiens 4-8 24471412-2 2014 The SphK isoforms (SphK1 and SphK2) catalyze the conversion of the proapoptotic substrate d-erythrosphingosine to the promitogenic/migratory product sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 149-172 sphingosine kinase 1 Homo sapiens 19-24 24471412-2 2014 The SphK isoforms (SphK1 and SphK2) catalyze the conversion of the proapoptotic substrate d-erythrosphingosine to the promitogenic/migratory product sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 174-177 sphingosine kinase 1 Homo sapiens 4-8 24471412-2 2014 The SphK isoforms (SphK1 and SphK2) catalyze the conversion of the proapoptotic substrate d-erythrosphingosine to the promitogenic/migratory product sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 174-177 sphingosine kinase 1 Homo sapiens 19-24 24792926-3 2014 The assay conveniently follows SphK-dependent changes in 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD)-labeled sphingosine (Sph) fluorescence and can be easily performed in 384-well plate format with small reaction volumes. 7-nitro-2-1,3-benzoxadiazol-4-yl 57-89 sphingosine kinase 1 Homo sapiens 31-35 24792926-3 2014 The assay conveniently follows SphK-dependent changes in 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD)-labeled sphingosine (Sph) fluorescence and can be easily performed in 384-well plate format with small reaction volumes. nbd 91-94 sphingosine kinase 1 Homo sapiens 31-35 24792926-3 2014 The assay conveniently follows SphK-dependent changes in 7-nitro-2-1,3-benzoxadiazol-4-yl (NBD)-labeled sphingosine (Sph) fluorescence and can be easily performed in 384-well plate format with small reaction volumes. Sphingosine 104-115 sphingosine kinase 1 Homo sapiens 31-35 24792926-5 2014 The SphK1 and SphK2 binding affinities for NBD-Sph and the IC50 values of inhibitors determined were consistent with those reported with other methods. nbd-sph 43-50 sphingosine kinase 1 Homo sapiens 4-9 24768661-1 2014 We recently discovered that a signaling lipid, sphingosine-1-phosphate (S1P), generated by sphingosine kinase 1, regulates a major epidermal antimicrobial peptide"s [cathelicidin antimicrobial peptide (CAMP)] expression via an NF-kappaB C/EBPalpha-dependent pathway, independent of vitamin D receptor (VDR) in epithelial cells. sphingosine 1-phosphate 47-70 sphingosine kinase 1 Homo sapiens 91-111 25111230-1 2014 Sphingosine-1-phosphate (S1P) is produced by sphingosine kinase 1 and is implicated in tumor growth, although the mechanisms remain incompletely understood. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 45-65 25150091-1 2014 Sphingosine kinases (SphK1, SphK2) are main regulators of sphingosine-1-phosphate (S1P), which is a pleiotropic lipid mediator involved in numerous physiological and pathophysiological functions. sphingosine 1-phosphate 58-81 sphingosine kinase 1 Homo sapiens 21-26 25150091-5 2014 We identified N-(4-methylthiazol-2-yl)-(2,4"-bithiazol)-2"-amine (24, ST-1803; IC50 values: 7.3 muM (SphK1), 6.5 muM (SphK2)) as a promising candidate for further in vivo investigations and structural development. n-(4-methylthiazol-2-yl)-(2,4"-bithiazol)-2"-amine 14-64 sphingosine kinase 1 Homo sapiens 101-106 25109605-1 2014 We previously reported that sphingosine kinase 1 (SphK1), an enzyme that catalyzes the production of sphingosine-1-phosphate (SIP), is upregulated in human gastric cancer and predicts poor clinical outcome. sphingosine 1-phosphate 101-124 sphingosine kinase 1 Homo sapiens 28-48 25109605-1 2014 We previously reported that sphingosine kinase 1 (SphK1), an enzyme that catalyzes the production of sphingosine-1-phosphate (SIP), is upregulated in human gastric cancer and predicts poor clinical outcome. sphingosine 1-phosphate 101-124 sphingosine kinase 1 Homo sapiens 50-55 25109605-1 2014 We previously reported that sphingosine kinase 1 (SphK1), an enzyme that catalyzes the production of sphingosine-1-phosphate (SIP), is upregulated in human gastric cancer and predicts poor clinical outcome. sphingosine 1-phosphate 126-129 sphingosine kinase 1 Homo sapiens 28-48 25109605-1 2014 We previously reported that sphingosine kinase 1 (SphK1), an enzyme that catalyzes the production of sphingosine-1-phosphate (SIP), is upregulated in human gastric cancer and predicts poor clinical outcome. sphingosine 1-phosphate 126-129 sphingosine kinase 1 Homo sapiens 50-55 25226616-1 2014 Sphingosine 1-phosphate (S1P) is a bioactive lipid that is formed by the phosphorylation of sphingosine and catalysed by sphingosine kinase 1 (SK1) or sphingosine kinase 2 (SK2). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 121-141 25226616-1 2014 Sphingosine 1-phosphate (S1P) is a bioactive lipid that is formed by the phosphorylation of sphingosine and catalysed by sphingosine kinase 1 (SK1) or sphingosine kinase 2 (SK2). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 143-146 24399507-0 2014 Sphingosine kinase 1 and sphingosine-1-phosphate in oxidative stress evoked by 1-methyl-4-phenylpyridinium (MPP+) in human dopaminergic neuronal cells. 1-Methyl-4-phenylpyridinium 79-106 sphingosine kinase 1 Homo sapiens 0-20 24399507-0 2014 Sphingosine kinase 1 and sphingosine-1-phosphate in oxidative stress evoked by 1-methyl-4-phenylpyridinium (MPP+) in human dopaminergic neuronal cells. mangion-purified polysaccharide (Candida albicans) 108-112 sphingosine kinase 1 Homo sapiens 0-20 24399507-1 2014 Sphingosine kinases (Sphk1/2) are crucial enzymes in regulation of the biostat between sphingosine-1-phosphate (S1P) and ceramide and play an important role in the pathogenesis/pathomechanism of Alzheimer"s disease (AD). sphingosine 1-phosphate 87-110 sphingosine kinase 1 Homo sapiens 21-28 24399507-1 2014 Sphingosine kinases (Sphk1/2) are crucial enzymes in regulation of the biostat between sphingosine-1-phosphate (S1P) and ceramide and play an important role in the pathogenesis/pathomechanism of Alzheimer"s disease (AD). sphingosine 1-phosphate 112-115 sphingosine kinase 1 Homo sapiens 21-28 24399507-1 2014 Sphingosine kinases (Sphk1/2) are crucial enzymes in regulation of the biostat between sphingosine-1-phosphate (S1P) and ceramide and play an important role in the pathogenesis/pathomechanism of Alzheimer"s disease (AD). Ceramides 121-129 sphingosine kinase 1 Homo sapiens 21-28 24399507-5 2014 The aim of our study was to investigate the expression level of Sphk1 and its role in human dopaminergic neuronal cell (SH-SY5Y) viability under oxidative stress, evoked by 1-methyl-4-phenylpyridinium (MPP+). 1-Methyl-4-phenylpyridinium 173-200 sphingosine kinase 1 Homo sapiens 64-69 24399507-5 2014 The aim of our study was to investigate the expression level of Sphk1 and its role in human dopaminergic neuronal cell (SH-SY5Y) viability under oxidative stress, evoked by 1-methyl-4-phenylpyridinium (MPP+). mangion-purified polysaccharide (Candida albicans) 202-206 sphingosine kinase 1 Homo sapiens 64-69 24399507-8 2014 Inhibition of Sphk1 decreased SH-SY5Y cell viability and concomitantly enhanced the reactive oxygen species (ROS) level. Reactive Oxygen Species 84-107 sphingosine kinase 1 Homo sapiens 14-19 24399507-8 2014 Inhibition of Sphk1 decreased SH-SY5Y cell viability and concomitantly enhanced the reactive oxygen species (ROS) level. Reactive Oxygen Species 109-112 sphingosine kinase 1 Homo sapiens 14-19 25010828-1 2014 Phosphorylation of sphingosine by sphingosine kinases (SphK1 and SphK2) generates sphingosine-1-phosphate (S1P), a bioactive sphingolipid which promotes cancer cell survival and tumor progression in vivo. Sphingosine 19-30 sphingosine kinase 1 Homo sapiens 55-60 25010828-1 2014 Phosphorylation of sphingosine by sphingosine kinases (SphK1 and SphK2) generates sphingosine-1-phosphate (S1P), a bioactive sphingolipid which promotes cancer cell survival and tumor progression in vivo. sphingosine 1-phosphate 82-105 sphingosine kinase 1 Homo sapiens 55-60 25010828-1 2014 Phosphorylation of sphingosine by sphingosine kinases (SphK1 and SphK2) generates sphingosine-1-phosphate (S1P), a bioactive sphingolipid which promotes cancer cell survival and tumor progression in vivo. sphingosine 1-phosphate 107-110 sphingosine kinase 1 Homo sapiens 55-60 25010828-1 2014 Phosphorylation of sphingosine by sphingosine kinases (SphK1 and SphK2) generates sphingosine-1-phosphate (S1P), a bioactive sphingolipid which promotes cancer cell survival and tumor progression in vivo. Sphingolipids 125-137 sphingosine kinase 1 Homo sapiens 55-60 23873031-2 2014 have demonstrated a critical role of melanoma sphingosine kinase 1, which catalyses formation of sphingosine 1-phosphate (S1P), in promoting the differentiation of fibroblasts into myofibroblasts. sphingosine 1-phosphate 97-120 sphingosine kinase 1 Homo sapiens 46-66 25197261-2 2014 Sphingosine kinase1 (SphK1), an enzyme in sphingolipid metabolism, is emerging as a key player in HNSCC pathology. Sphingolipids 42-54 sphingosine kinase 1 Homo sapiens 0-19 25197261-2 2014 Sphingosine kinase1 (SphK1), an enzyme in sphingolipid metabolism, is emerging as a key player in HNSCC pathology. Sphingolipids 42-54 sphingosine kinase 1 Homo sapiens 21-26 24768446-0 2014 Coumestrol suppresses hypoxia inducible factor 1alpha by inhibiting ROS mediated sphingosine kinase 1 in hypoxic PC-3 prostate cancer cells. Coumestrol 0-10 sphingosine kinase 1 Homo sapiens 81-101 24768446-6 2014 Overall, our findings suggest that coumestrol suppresses the accumulation of HIF-1alpha via suppression of SPHK1 pathway in hypoxic PC-3 cells. Coumestrol 35-45 sphingosine kinase 1 Homo sapiens 107-112 24657936-3 2014 Sphingosine kinase (SphK1) activity can alter the sphingosine-1-phosphate (S1P)/ceramide ratio leading to an imbalance in the sphingolipid rheostat. sphingosine 1-phosphate 50-73 sphingosine kinase 1 Homo sapiens 20-25 24657936-3 2014 Sphingosine kinase (SphK1) activity can alter the sphingosine-1-phosphate (S1P)/ceramide ratio leading to an imbalance in the sphingolipid rheostat. sphingosine 1-phosphate 75-78 sphingosine kinase 1 Homo sapiens 20-25 24657936-3 2014 Sphingosine kinase (SphK1) activity can alter the sphingosine-1-phosphate (S1P)/ceramide ratio leading to an imbalance in the sphingolipid rheostat. Ceramides 80-88 sphingosine kinase 1 Homo sapiens 20-25 24657936-4 2014 Fingolimod (FTY720) is a novel sphingosine analog and a potent immunosuppressive drug that acts as a SphK1 antagonist, inhibits the growth, and induces apoptosis in different human cancer cell lines. Fingolimod Hydrochloride 0-10 sphingosine kinase 1 Homo sapiens 101-106 24657936-4 2014 Fingolimod (FTY720) is a novel sphingosine analog and a potent immunosuppressive drug that acts as a SphK1 antagonist, inhibits the growth, and induces apoptosis in different human cancer cell lines. Fingolimod Hydrochloride 12-18 sphingosine kinase 1 Homo sapiens 101-106 24768446-0 2014 Coumestrol suppresses hypoxia inducible factor 1alpha by inhibiting ROS mediated sphingosine kinase 1 in hypoxic PC-3 prostate cancer cells. Reactive Oxygen Species 68-71 sphingosine kinase 1 Homo sapiens 81-101 24768446-2 2014 Thus, in the present study, molecular mechanisms of coumestrol were investigated on the SPHK1 and HIF-1alpha signaling pathway in hypoxic PC-3 prostate cancer cells. Coumestrol 52-62 sphingosine kinase 1 Homo sapiens 88-93 24768446-3 2014 Coumestrol significantly suppressed SPHK1 activity and accumulation of HIF-1alpha in a time- and concentration-dependent manner in hypoxic PC-3 cells. Coumestrol 0-10 sphingosine kinase 1 Homo sapiens 36-41 24768446-5 2014 Furthermore, SPHK1 siRNA transfection, sphigosine kinase inhibitor (SKI), reactive oxygen species (ROS) enhanced the inhibitory effect of coumestrol on the accumulation of HIF-1alpha and the expression of pAKT and pGSK 3beta in hypoxic PC-3 cells by combination index. Reactive Oxygen Species 74-97 sphingosine kinase 1 Homo sapiens 13-18 24768446-5 2014 Furthermore, SPHK1 siRNA transfection, sphigosine kinase inhibitor (SKI), reactive oxygen species (ROS) enhanced the inhibitory effect of coumestrol on the accumulation of HIF-1alpha and the expression of pAKT and pGSK 3beta in hypoxic PC-3 cells by combination index. Reactive Oxygen Species 99-102 sphingosine kinase 1 Homo sapiens 13-18 24768446-5 2014 Furthermore, SPHK1 siRNA transfection, sphigosine kinase inhibitor (SKI), reactive oxygen species (ROS) enhanced the inhibitory effect of coumestrol on the accumulation of HIF-1alpha and the expression of pAKT and pGSK 3beta in hypoxic PC-3 cells by combination index. Coumestrol 138-148 sphingosine kinase 1 Homo sapiens 13-18 24768446-5 2014 Furthermore, SPHK1 siRNA transfection, sphigosine kinase inhibitor (SKI), reactive oxygen species (ROS) enhanced the inhibitory effect of coumestrol on the accumulation of HIF-1alpha and the expression of pAKT and pGSK 3beta in hypoxic PC-3 cells by combination index. pgsk 3beta 214-224 sphingosine kinase 1 Homo sapiens 13-18 24587339-1 2014 BACKGROUND: Sphingosine kinase 1 (SK1) is a key regulator of the dynamic ceramide/sphingosine 1-phosphate rheostat balance and important in the pathological cancer genesis, progression, and metastasis processes. Ceramides 73-81 sphingosine kinase 1 Homo sapiens 12-32 24970218-2 2014 Increased expression of sphingosine kinase 1 (SPHK1), which catalyses the synthesis of the pro-survival, pro-angiogenic metabolite sphingosine 1-phosphate (S1P), is well established as a hallmark of multiple cancers. sphingosine 1-phosphate 131-154 sphingosine kinase 1 Homo sapiens 24-44 24970218-2 2014 Increased expression of sphingosine kinase 1 (SPHK1), which catalyses the synthesis of the pro-survival, pro-angiogenic metabolite sphingosine 1-phosphate (S1P), is well established as a hallmark of multiple cancers. sphingosine 1-phosphate 131-154 sphingosine kinase 1 Homo sapiens 46-51 23975598-6 2014 In addition, we found that both SK1 and ERK were activated in Sunitinib-resistance cell lines and SK1 and ERK inhibitors could resensitize Sunitinib-resistant cell lines. Sunitinib 62-71 sphingosine kinase 1 Homo sapiens 32-35 23975598-6 2014 In addition, we found that both SK1 and ERK were activated in Sunitinib-resistance cell lines and SK1 and ERK inhibitors could resensitize Sunitinib-resistant cell lines. Sunitinib 139-148 sphingosine kinase 1 Homo sapiens 32-35 23975598-6 2014 In addition, we found that both SK1 and ERK were activated in Sunitinib-resistance cell lines and SK1 and ERK inhibitors could resensitize Sunitinib-resistant cell lines. Sunitinib 139-148 sphingosine kinase 1 Homo sapiens 98-101 23975598-7 2014 In conclusion, our observations suggest that SK1 and ERK activation is a feature of resistant cell lines, which serves as an alternative pathway evading anti-tumor activity of Sunitinib. Sunitinib 176-185 sphingosine kinase 1 Homo sapiens 45-48 24572701-1 2014 OBJECTIVES: Sphingosine kinase 1 (SphK1) phosphorylates the membrane sphingolipid, sphingosine, to sphingosine-1-phosphate (S1P), an oncogenic mediator, which drives tumor cell growth and survival. Sphingolipids 69-81 sphingosine kinase 1 Homo sapiens 12-32 24572701-1 2014 OBJECTIVES: Sphingosine kinase 1 (SphK1) phosphorylates the membrane sphingolipid, sphingosine, to sphingosine-1-phosphate (S1P), an oncogenic mediator, which drives tumor cell growth and survival. Sphingolipids 69-81 sphingosine kinase 1 Homo sapiens 34-39 24572701-1 2014 OBJECTIVES: Sphingosine kinase 1 (SphK1) phosphorylates the membrane sphingolipid, sphingosine, to sphingosine-1-phosphate (S1P), an oncogenic mediator, which drives tumor cell growth and survival. Sphingosine 83-94 sphingosine kinase 1 Homo sapiens 12-32 24572701-1 2014 OBJECTIVES: Sphingosine kinase 1 (SphK1) phosphorylates the membrane sphingolipid, sphingosine, to sphingosine-1-phosphate (S1P), an oncogenic mediator, which drives tumor cell growth and survival. Sphingosine 83-94 sphingosine kinase 1 Homo sapiens 34-39 24572701-1 2014 OBJECTIVES: Sphingosine kinase 1 (SphK1) phosphorylates the membrane sphingolipid, sphingosine, to sphingosine-1-phosphate (S1P), an oncogenic mediator, which drives tumor cell growth and survival. sphingosine 1-phosphate 99-122 sphingosine kinase 1 Homo sapiens 12-32 24572701-1 2014 OBJECTIVES: Sphingosine kinase 1 (SphK1) phosphorylates the membrane sphingolipid, sphingosine, to sphingosine-1-phosphate (S1P), an oncogenic mediator, which drives tumor cell growth and survival. sphingosine 1-phosphate 99-122 sphingosine kinase 1 Homo sapiens 34-39 24572701-1 2014 OBJECTIVES: Sphingosine kinase 1 (SphK1) phosphorylates the membrane sphingolipid, sphingosine, to sphingosine-1-phosphate (S1P), an oncogenic mediator, which drives tumor cell growth and survival. sphingosine 1-phosphate 124-127 sphingosine kinase 1 Homo sapiens 12-32 24572701-1 2014 OBJECTIVES: Sphingosine kinase 1 (SphK1) phosphorylates the membrane sphingolipid, sphingosine, to sphingosine-1-phosphate (S1P), an oncogenic mediator, which drives tumor cell growth and survival. sphingosine 1-phosphate 124-127 sphingosine kinase 1 Homo sapiens 34-39 24572701-10 2014 Inhibition of SphK1 also enhanced the sensitivity of colon cancer cells to 5-FU. Fluorouracil 75-79 sphingosine kinase 1 Homo sapiens 14-19 24587339-1 2014 BACKGROUND: Sphingosine kinase 1 (SK1) is a key regulator of the dynamic ceramide/sphingosine 1-phosphate rheostat balance and important in the pathological cancer genesis, progression, and metastasis processes. Ceramides 73-81 sphingosine kinase 1 Homo sapiens 34-37 24587339-1 2014 BACKGROUND: Sphingosine kinase 1 (SK1) is a key regulator of the dynamic ceramide/sphingosine 1-phosphate rheostat balance and important in the pathological cancer genesis, progression, and metastasis processes. sphingosine 1-phosphate 82-105 sphingosine kinase 1 Homo sapiens 12-32 24587339-1 2014 BACKGROUND: Sphingosine kinase 1 (SK1) is a key regulator of the dynamic ceramide/sphingosine 1-phosphate rheostat balance and important in the pathological cancer genesis, progression, and metastasis processes. sphingosine 1-phosphate 82-105 sphingosine kinase 1 Homo sapiens 34-37 24456642-3 2014 S1P is derived by phosphorylation of sphingosine, catalysed by sphingosine kinases 1 and 2 (SphK1 and 2), and SphK1 positively regulates glutamate secretion and synaptic strength in hippocampal neurons. Sphingosine 37-48 sphingosine kinase 1 Homo sapiens 63-90 24456642-3 2014 S1P is derived by phosphorylation of sphingosine, catalysed by sphingosine kinases 1 and 2 (SphK1 and 2), and SphK1 positively regulates glutamate secretion and synaptic strength in hippocampal neurons. Sphingosine 37-48 sphingosine kinase 1 Homo sapiens 92-103 24456642-3 2014 S1P is derived by phosphorylation of sphingosine, catalysed by sphingosine kinases 1 and 2 (SphK1 and 2), and SphK1 positively regulates glutamate secretion and synaptic strength in hippocampal neurons. Sphingosine 37-48 sphingosine kinase 1 Homo sapiens 92-97 24070975-1 2014 The enzymes that catalyze formation of the bioactive sphingolipid, sphingosine 1-phosphate, sphingosine kinase 1 and 2, are predictive markers in inflammatory diseases and cancer as evidenced by data from patients, knockout mice and the use of available molecular and chemical inhibitors. Sphingolipids 53-65 sphingosine kinase 1 Homo sapiens 67-118 23789924-3 2014 In this MiniReview, we discuss the possibility of targeting sphingolipids, specifically the sphingosine kinase 1 (SphK1) and sphingosine 1-phosphate (S1P) pathway, as new therapeutic strategy for the treatment of glomerulonephritis, as this pathway was demonstrated to be dysregulated under disease conditions. Sphingolipids 60-73 sphingosine kinase 1 Homo sapiens 92-112 23789924-3 2014 In this MiniReview, we discuss the possibility of targeting sphingolipids, specifically the sphingosine kinase 1 (SphK1) and sphingosine 1-phosphate (S1P) pathway, as new therapeutic strategy for the treatment of glomerulonephritis, as this pathway was demonstrated to be dysregulated under disease conditions. Sphingolipids 60-73 sphingosine kinase 1 Homo sapiens 114-119 23861887-3 2013 According to the proposed rheostat theory, SPHK activity shifts the intracellular balance from the pro-apoptotic sphingolipids ceramide and sphingosine to the mitogenic S1P, thereby determining the susceptibility of a cell to apoptotic stress. Sphingolipids 113-126 sphingosine kinase 1 Homo sapiens 43-47 24687256-3 2014 This study examined the anti-angiogenic properties of CK against sphingosine 1-phosphate (S1P)-induced cell migration via regulation of sphingosine kinase 1 (SPHK1) in human umbilical vein endothelial cells (HUVEC). ginsenoside M1 54-56 sphingosine kinase 1 Homo sapiens 136-156 24687256-3 2014 This study examined the anti-angiogenic properties of CK against sphingosine 1-phosphate (S1P)-induced cell migration via regulation of sphingosine kinase 1 (SPHK1) in human umbilical vein endothelial cells (HUVEC). ginsenoside M1 54-56 sphingosine kinase 1 Homo sapiens 158-163 24687256-5 2014 S1P produced by SPHK1 is also involved in cell growth, migration, and protection of apoptosis; therefore, we sought to investigate whether ginsenosides are able to regulate SPHK1. Ginsenosides 139-151 sphingosine kinase 1 Homo sapiens 173-178 24687256-8 2014 Among tested ginsenosides, CK exclusively inhibited S1P production, SPHK1 activity and SPHK1 expression in HUVEC, whereas expression of the pro-apoptotic sphingolipids, sphingosine and ceramide, was increased in response to CK. ginsenoside M1 27-29 sphingosine kinase 1 Homo sapiens 68-73 24687256-8 2014 Among tested ginsenosides, CK exclusively inhibited S1P production, SPHK1 activity and SPHK1 expression in HUVEC, whereas expression of the pro-apoptotic sphingolipids, sphingosine and ceramide, was increased in response to CK. ginsenoside M1 27-29 sphingosine kinase 1 Homo sapiens 87-92 24687256-11 2014 Ginsenoside CK acts as a unique HUVEC migration inhibitor by regulating MMP expression, as well as the activity of SPHK1 and its related sphingolipid metabolites. ginsenoside M1 0-14 sphingosine kinase 1 Homo sapiens 115-120 24687256-11 2014 Ginsenoside CK acts as a unique HUVEC migration inhibitor by regulating MMP expression, as well as the activity of SPHK1 and its related sphingolipid metabolites. Sphingolipids 137-149 sphingosine kinase 1 Homo sapiens 115-120 24376889-0 2013 Effects of inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate induced insulin resistance in L6 myotubes. Palmitates 95-104 sphingosine kinase 1 Homo sapiens 63-83 24376889-0 2013 Effects of inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate induced insulin resistance in L6 myotubes. Palmitates 95-104 sphingosine kinase 1 Homo sapiens 85-90 24376889-1 2013 BACKGROUND: The objective of this study was to examine the effects of short (2 h) and prolonged (18 h) inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate (PA) induced insulin resistance in L6 myotubes. Palmitates 187-196 sphingosine kinase 1 Homo sapiens 155-175 24376889-1 2013 BACKGROUND: The objective of this study was to examine the effects of short (2 h) and prolonged (18 h) inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate (PA) induced insulin resistance in L6 myotubes. Palmitates 187-196 sphingosine kinase 1 Homo sapiens 177-182 24376889-1 2013 BACKGROUND: The objective of this study was to examine the effects of short (2 h) and prolonged (18 h) inhibition of serine palmitoyltransferase (SPT) and sphingosine kinase 1 (SphK1) on palmitate (PA) induced insulin resistance in L6 myotubes. Palmitates 198-200 sphingosine kinase 1 Homo sapiens 177-182 24376889-7 2013 In contrast, prolonged inhibition of SphK1 intensified the effect of PA on insulin-stimulated glucose uptake and attenuated further the activity of insulin signaling proteins (pGSK3beta/GSK3beta ratio) in L6 myotubes. Palmitates 69-71 sphingosine kinase 1 Homo sapiens 37-42 24376889-7 2013 In contrast, prolonged inhibition of SphK1 intensified the effect of PA on insulin-stimulated glucose uptake and attenuated further the activity of insulin signaling proteins (pGSK3beta/GSK3beta ratio) in L6 myotubes. Glucose 94-101 sphingosine kinase 1 Homo sapiens 37-42 24376889-9 2013 CONCLUSION: Myriocin is more effective in restoration of palmitate induced insulin resistance in L6 myocytes, despite of the time of SPT inhibition, comparing to SKII (a specific SphK1 inhibitor). thermozymocidin 12-20 sphingosine kinase 1 Homo sapiens 179-184 24376889-11 2013 Interestingly, inactivation of SphK1 augmented the effect of PA induced insulin resistance in L6 myotubes, which was associated with further inhibition of insulin stimulated PKB and GSK3beta phosphorylation, glucose uptake and the accumulation of sphingosine. Palmitates 61-63 sphingosine kinase 1 Homo sapiens 31-36 24376889-11 2013 Interestingly, inactivation of SphK1 augmented the effect of PA induced insulin resistance in L6 myotubes, which was associated with further inhibition of insulin stimulated PKB and GSK3beta phosphorylation, glucose uptake and the accumulation of sphingosine. Glucose 208-215 sphingosine kinase 1 Homo sapiens 31-36 24376889-11 2013 Interestingly, inactivation of SphK1 augmented the effect of PA induced insulin resistance in L6 myotubes, which was associated with further inhibition of insulin stimulated PKB and GSK3beta phosphorylation, glucose uptake and the accumulation of sphingosine. Sphingosine 247-258 sphingosine kinase 1 Homo sapiens 31-36 23928990-7 2013 Notably, we show that estradiol (E2) treatment downregulates miR-515-5p levels, whereas the antiestrogen tamoxifen causes a decrease in SK1, which is rescued by silencing miR-515-5p. Tamoxifen 105-114 sphingosine kinase 1 Homo sapiens 136-139 23935096-1 2013 Sphingosine kinase 1 (SK1) produces the pro-survival sphingolipid sphingosine 1-phosphate and has been implicated in inflammation, proliferation, and angiogenesis. sphingolipid sphingosine 1-phosphate 53-89 sphingosine kinase 1 Homo sapiens 0-25 23935096-2 2013 Recent studies identified TRAF2 as a sphingosine 1-phosphate target, implicating SK1 in activation of the NF-kappaB pathway, but the functional consequences of this connection on gene expression are unknown. sphingosine 1-phosphate 37-60 sphingosine kinase 1 Homo sapiens 81-84 23658376-1 2013 AIMS: Sphingosine-1-phosphate (S1P) is a cellular signalling lipid generated by sphingosine kinase-1 (SPHK1). sphingosine 1-phosphate 6-29 sphingosine kinase 1 Homo sapiens 80-100 23658376-1 2013 AIMS: Sphingosine-1-phosphate (S1P) is a cellular signalling lipid generated by sphingosine kinase-1 (SPHK1). sphingosine 1-phosphate 6-29 sphingosine kinase 1 Homo sapiens 102-107 23658376-1 2013 AIMS: Sphingosine-1-phosphate (S1P) is a cellular signalling lipid generated by sphingosine kinase-1 (SPHK1). sphingosine 1-phosphate 31-34 sphingosine kinase 1 Homo sapiens 80-100 23658376-1 2013 AIMS: Sphingosine-1-phosphate (S1P) is a cellular signalling lipid generated by sphingosine kinase-1 (SPHK1). sphingosine 1-phosphate 31-34 sphingosine kinase 1 Homo sapiens 102-107 23839903-0 2013 Hepatopoietin Cn reduces ethanol-induced hepatoxicity via sphingosine kinase 1 and sphingosine 1-phosphate receptors. Ethanol 25-32 sphingosine kinase 1 Homo sapiens 58-116 23839903-2 2013 Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid produced by sphingosine kinase (SphK). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 70-88 23839903-2 2013 Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid produced by sphingosine kinase (SphK). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 90-94 23839903-2 2013 Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid produced by sphingosine kinase (SphK). sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 70-88 23839903-2 2013 Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid produced by sphingosine kinase (SphK). sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 90-94 23839903-2 2013 Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid produced by sphingosine kinase (SphK). Sphingolipids 45-57 sphingosine kinase 1 Homo sapiens 70-88 23839903-2 2013 Sphingosine 1-phosphate (S1P) is a bioactive sphingolipid produced by sphingosine kinase (SphK). Sphingolipids 45-57 sphingosine kinase 1 Homo sapiens 90-94 23839903-4 2013 This work sought to understand the mechanism by which SphK/S1P/S1PRs are involved in the protective effects of HPPCn on ethanol-induced liver injury and fibrosis. Ethanol 120-127 sphingosine kinase 1 Homo sapiens 54-58 25310737-1 2014 Sphingosine kinases (Sphk1/2 EC 2.7.1.91) are responsible for synthesis of sphingosine-1-phosphate (S1P) and for regulation of the bioactive sphingolipids homeostasis. sphingosine 1-phosphate 75-98 sphingosine kinase 1 Homo sapiens 21-26 25310737-1 2014 Sphingosine kinases (Sphk1/2 EC 2.7.1.91) are responsible for synthesis of sphingosine-1-phosphate (S1P) and for regulation of the bioactive sphingolipids homeostasis. sphingosine 1-phosphate 100-103 sphingosine kinase 1 Homo sapiens 21-26 25310737-1 2014 Sphingosine kinases (Sphk1/2 EC 2.7.1.91) are responsible for synthesis of sphingosine-1-phosphate (S1P) and for regulation of the bioactive sphingolipids homeostasis. Sphingolipids 141-154 sphingosine kinase 1 Homo sapiens 21-26 25310737-6 2014 Our latest data demonstrated significant inhibition of Sphk1 gene expression and activity in an in vitro PD model, induced by 1-methyl-4-phenylpyridinium (MPP+). 1-Methyl-4-phenylpyridinium 126-153 sphingosine kinase 1 Homo sapiens 55-60 25310737-6 2014 Our latest data demonstrated significant inhibition of Sphk1 gene expression and activity in an in vitro PD model, induced by 1-methyl-4-phenylpyridinium (MPP+). mangion-purified polysaccharide (Candida albicans) 155-159 sphingosine kinase 1 Homo sapiens 55-60 24173614-0 2014 DMS triggers apoptosis associated with the inhibition of SPHK1/NF-kappaB activation and increase in intracellular Ca2+ concentration in human cancer cells. N,N-dimethylsphingosine 0-3 sphingosine kinase 1 Homo sapiens 57-62 24173614-1 2014 N,N-Dimethyl-D-erythro-sphingosine (DMS) is known to induce cell apoptosis by specifically inhibiting sphingosine kinase 1 (SPHK1) and modulating the activity of cellular ceramide levels. N,N-dimethylsphingosine 0-34 sphingosine kinase 1 Homo sapiens 102-122 24173614-1 2014 N,N-Dimethyl-D-erythro-sphingosine (DMS) is known to induce cell apoptosis by specifically inhibiting sphingosine kinase 1 (SPHK1) and modulating the activity of cellular ceramide levels. N,N-dimethylsphingosine 0-34 sphingosine kinase 1 Homo sapiens 124-129 24173614-1 2014 N,N-Dimethyl-D-erythro-sphingosine (DMS) is known to induce cell apoptosis by specifically inhibiting sphingosine kinase 1 (SPHK1) and modulating the activity of cellular ceramide levels. N,N-dimethylsphingosine 36-39 sphingosine kinase 1 Homo sapiens 102-122 24173614-1 2014 N,N-Dimethyl-D-erythro-sphingosine (DMS) is known to induce cell apoptosis by specifically inhibiting sphingosine kinase 1 (SPHK1) and modulating the activity of cellular ceramide levels. N,N-dimethylsphingosine 36-39 sphingosine kinase 1 Homo sapiens 124-129 24173614-4 2014 Mechanistically, treatment with DMS suppressed the activation of SPHK1 and nuclear factor-kappaB (NF-kappaB) p65, but increased intracellular [Ca2+]i in A549 cells. N,N-dimethylsphingosine 32-35 sphingosine kinase 1 Homo sapiens 65-96 24140934-1 2014 Sphingosine kinase (SPHK) is overexpressed by a variety of cancers, and its phosphorylation of sphingosine results in accumulation of sphingosine-1-phosphate (S1P) and activation of antiapoptotic signal transduction. Sphingosine 95-106 sphingosine kinase 1 Homo sapiens 20-24 24140934-1 2014 Sphingosine kinase (SPHK) is overexpressed by a variety of cancers, and its phosphorylation of sphingosine results in accumulation of sphingosine-1-phosphate (S1P) and activation of antiapoptotic signal transduction. sphingosine 1-phosphate 134-157 sphingosine kinase 1 Homo sapiens 20-24 24170779-5 2014 Acting in an autocrine/paracrine manner in macrophages, PGE2 induces a regulatory phenotype including the expression of interleukin (IL)-10, sphingosine kinase 1, and the tumor necrosis factor family molecule LIGHT. Dinoprostone 56-60 sphingosine kinase 1 Homo sapiens 141-161 24164513-1 2013 The design, synthesis, and evaluation of the potency of new isoform-selective inhibitors of sphingosine kinases 1 and 2 (SK1 and SK2), the enzyme that catalyzes the phosphorylation of d-erythro-sphingosine to produce the key signaling lipid, sphingosine 1-phosphate, are described. Sphingosine 184-205 sphingosine kinase 1 Homo sapiens 121-124 24164513-1 2013 The design, synthesis, and evaluation of the potency of new isoform-selective inhibitors of sphingosine kinases 1 and 2 (SK1 and SK2), the enzyme that catalyzes the phosphorylation of d-erythro-sphingosine to produce the key signaling lipid, sphingosine 1-phosphate, are described. sphingosine 1-phosphate 242-265 sphingosine kinase 1 Homo sapiens 121-124 24164513-2 2013 Recently, we reported that 1-(4-octylphenethyl)piperidin-4-ol (RB-005) is a selective inhibitor of SK1. 1-(4-octylphenethyl)piperidin-4-ol 27-61 sphingosine kinase 1 Homo sapiens 99-102 24164513-2 2013 Recently, we reported that 1-(4-octylphenethyl)piperidin-4-ol (RB-005) is a selective inhibitor of SK1. 1-(4-octylphenethyl)piperidin-4-ol 63-69 sphingosine kinase 1 Homo sapiens 99-102 24164513-3 2013 Here we report the synthesis of 43 new analogues of RB-005, in which the lipophilic tail, polar headgroup, and linker region were modified to extend the structure-activity relationship profile for this lead compound, which we explain using modeling studies with the recently published crystal structure of SK1. Rubidium 52-54 sphingosine kinase 1 Homo sapiens 306-309 24970177-4 2013 This review offers insight into the role of sphingosine kinase-1 (SphK1), a key enzyme in sphingolipid metabolism, in HNSCC. Sphingolipids 90-102 sphingosine kinase 1 Homo sapiens 44-64 24970177-4 2013 This review offers insight into the role of sphingosine kinase-1 (SphK1), a key enzyme in sphingolipid metabolism, in HNSCC. Sphingolipids 90-102 sphingosine kinase 1 Homo sapiens 66-71 23651497-0 2013 A signaling cascade mediated by ceramide, src and PDGFRbeta coordinates the activation of the redox-sensitive neutral sphingomyelinase-2 and sphingosine kinase-1. Ceramides 32-40 sphingosine kinase 1 Homo sapiens 141-161 23651497-2 2013 The two redox-sensitive neutral sphingomyelinase-2 (nSMase2) and sphingosine kinase-1 (SK1) participate in transducing stress signaling to ceramide and S1P, respectively; however, whether these key enzymes are coordinately regulated is not known. Ceramides 139-147 sphingosine kinase 1 Homo sapiens 65-90 23651497-3 2013 We investigated whether a signaling link coordinates nSMase2 and SK1 activation by H2O2. Hydrogen Peroxide 83-87 sphingosine kinase 1 Homo sapiens 65-68 23651497-5 2013 Low H2O2 concentration triggered activation of nSMase2 and SK1 through a nSMase2/Cer-dependent signaling pathway that acted upstream of activation of SK1. Hydrogen Peroxide 4-8 sphingosine kinase 1 Homo sapiens 59-62 23651497-5 2013 Low H2O2 concentration triggered activation of nSMase2 and SK1 through a nSMase2/Cer-dependent signaling pathway that acted upstream of activation of SK1. Hydrogen Peroxide 4-8 sphingosine kinase 1 Homo sapiens 150-153 23651497-7 2013 The H2O2-induced src/PDGFRbeta/SK1 signaling cascade was impaired in nSMase2-deficient fro/fro cells and was rescued by exogenous C2Cer that activated src/PDGFRbeta/SK1. Hydrogen Peroxide 4-8 sphingosine kinase 1 Homo sapiens 31-34 23651497-7 2013 The H2O2-induced src/PDGFRbeta/SK1 signaling cascade was impaired in nSMase2-deficient fro/fro cells and was rescued by exogenous C2Cer that activated src/PDGFRbeta/SK1. Hydrogen Peroxide 4-8 sphingosine kinase 1 Homo sapiens 165-168 23651497-11 2013 Taken together the results identify a tightly coordinated nSMase2/SK1 pathway that mediates the mitogenic effects of H2O2 and may sense the degree of oxidative stress. Hydrogen Peroxide 117-121 sphingosine kinase 1 Homo sapiens 66-69 23861887-3 2013 According to the proposed rheostat theory, SPHK activity shifts the intracellular balance from the pro-apoptotic sphingolipids ceramide and sphingosine to the mitogenic S1P, thereby determining the susceptibility of a cell to apoptotic stress. Ceramides 127-135 sphingosine kinase 1 Homo sapiens 43-47 23861887-3 2013 According to the proposed rheostat theory, SPHK activity shifts the intracellular balance from the pro-apoptotic sphingolipids ceramide and sphingosine to the mitogenic S1P, thereby determining the susceptibility of a cell to apoptotic stress. Sphingosine 140-151 sphingosine kinase 1 Homo sapiens 43-47 23098068-6 2013 There was an up-regulation of antiapoptotic BCR/ABL, GCS and SK-1 genes and MRP1 transporter gene and down-regulation of apoptotic Bax and CerS1 genes in nilotinib-resistant cells. nilotinib 154-163 sphingosine kinase 1 Homo sapiens 61-65 24069553-4 2013 in Cancer Cell demonstrate that sphingosine kinase 1 (SphK1)-mediated upregulation of sphingosine-1-phosphate (S1P) drives a persistent NFkappaB/IL-6/STAT3/sphingosine-1-phosphate receptor 1 (S1PR1) amplification loop that is critical to the development of chronic colitis and colitis-associated cancer. sphingosine 1-phosphate 86-109 sphingosine kinase 1 Homo sapiens 32-52 24069553-4 2013 in Cancer Cell demonstrate that sphingosine kinase 1 (SphK1)-mediated upregulation of sphingosine-1-phosphate (S1P) drives a persistent NFkappaB/IL-6/STAT3/sphingosine-1-phosphate receptor 1 (S1PR1) amplification loop that is critical to the development of chronic colitis and colitis-associated cancer. sphingosine 1-phosphate 86-109 sphingosine kinase 1 Homo sapiens 54-59 23426175-1 2013 Our previous study has shown that the activity and expression of sphingosine kinase (SPHK) regulated the sensitivity of human colon cancer cells to the chemotherapeutic oxaliplatin (L-OHP). Oxaliplatin 169-180 sphingosine kinase 1 Homo sapiens 65-83 23426175-1 2013 Our previous study has shown that the activity and expression of sphingosine kinase (SPHK) regulated the sensitivity of human colon cancer cells to the chemotherapeutic oxaliplatin (L-OHP). Oxaliplatin 169-180 sphingosine kinase 1 Homo sapiens 85-89 23426175-1 2013 Our previous study has shown that the activity and expression of sphingosine kinase (SPHK) regulated the sensitivity of human colon cancer cells to the chemotherapeutic oxaliplatin (L-OHP). Oxaliplatin 182-187 sphingosine kinase 1 Homo sapiens 65-83 23426175-1 2013 Our previous study has shown that the activity and expression of sphingosine kinase (SPHK) regulated the sensitivity of human colon cancer cells to the chemotherapeutic oxaliplatin (L-OHP). Oxaliplatin 182-187 sphingosine kinase 1 Homo sapiens 85-89 23426175-3 2013 Here, we use colon cancer cell lines to examine the relationship between SPHK1 activity and CD44 expression.CD44 expression was measured by western blotting and quantitative PCR in two human colon cancer cell lines: L-OHP-resistant RKO and L-OHP-sensitive HCT116. Oxaliplatin 216-221 sphingosine kinase 1 Homo sapiens 73-78 23098068-8 2013 Inhibiton of GCS and SK-1 restored nilotinib sensitivity. nilotinib 35-44 sphingosine kinase 1 Homo sapiens 21-25 24970169-6 2013 Great interest has also been shown in understanding the action of sphingosine kinase (SphK), i.e., the kinase phosphorylating sphingosine to S1P, and the interactions between S1P and growth factor signaling. Sphingosine 66-77 sphingosine kinase 1 Homo sapiens 86-90 23602659-1 2013 Sphingosine kinase 1 (SphK1) is a lipid kinase that catalyzes the conversion of sphingosine to sphingosine-1-phosphate (S1P), which has been shown to play a role in lymphocyte trafficking, angiogenesis, and response to apoptotic stimuli. Sphingosine 80-91 sphingosine kinase 1 Homo sapiens 0-20 23602659-1 2013 Sphingosine kinase 1 (SphK1) is a lipid kinase that catalyzes the conversion of sphingosine to sphingosine-1-phosphate (S1P), which has been shown to play a role in lymphocyte trafficking, angiogenesis, and response to apoptotic stimuli. Sphingosine 80-91 sphingosine kinase 1 Homo sapiens 22-27 23602659-1 2013 Sphingosine kinase 1 (SphK1) is a lipid kinase that catalyzes the conversion of sphingosine to sphingosine-1-phosphate (S1P), which has been shown to play a role in lymphocyte trafficking, angiogenesis, and response to apoptotic stimuli. sphingosine 1-phosphate 95-118 sphingosine kinase 1 Homo sapiens 0-20 23602659-1 2013 Sphingosine kinase 1 (SphK1) is a lipid kinase that catalyzes the conversion of sphingosine to sphingosine-1-phosphate (S1P), which has been shown to play a role in lymphocyte trafficking, angiogenesis, and response to apoptotic stimuli. sphingosine 1-phosphate 95-118 sphingosine kinase 1 Homo sapiens 22-27 23602659-3 2013 Here, we present the crystal structures of human SphK1 in the apo form and in complexes with a substrate sphingosine-like lipid, ADP, and an inhibitor at 2.0-2.3 A resolution. Sphingosine 105-116 sphingosine kinase 1 Homo sapiens 49-54 23602659-3 2013 Here, we present the crystal structures of human SphK1 in the apo form and in complexes with a substrate sphingosine-like lipid, ADP, and an inhibitor at 2.0-2.3 A resolution. Adenosine Diphosphate 129-132 sphingosine kinase 1 Homo sapiens 49-54 23602659-5 2013 Comparative analysis of these structures with mutagenesis and kinetic studies provides insight into how SphK1 recognizes the lipid substrate and catalyzes ATP-dependent phosphorylation. Adenosine Triphosphate 155-158 sphingosine kinase 1 Homo sapiens 104-109 23664359-1 2013 Sphingosine-1-phosphate is a potent sphingolipid mediator, and the kinase that produces it, sphingosine kinase 1 (SphK1), has been implicated in cancer progression, inflammation, and cardiovascular diseases. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 92-112 23664359-1 2013 Sphingosine-1-phosphate is a potent sphingolipid mediator, and the kinase that produces it, sphingosine kinase 1 (SphK1), has been implicated in cancer progression, inflammation, and cardiovascular diseases. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 114-119 23664359-1 2013 Sphingosine-1-phosphate is a potent sphingolipid mediator, and the kinase that produces it, sphingosine kinase 1 (SphK1), has been implicated in cancer progression, inflammation, and cardiovascular diseases. Sphingolipids 36-48 sphingosine kinase 1 Homo sapiens 92-112 23664359-1 2013 Sphingosine-1-phosphate is a potent sphingolipid mediator, and the kinase that produces it, sphingosine kinase 1 (SphK1), has been implicated in cancer progression, inflammation, and cardiovascular diseases. Sphingolipids 36-48 sphingosine kinase 1 Homo sapiens 114-119 24054007-2 2013 METHODS: Human colon cancer LoVo cells were divided into three groups: phorbol 12-myristate 13-acetate (PMA) was used to induce the activation of SphK1 in the PMA group, N,N-dimethylsphingosine (DMS) used to suppress the activity of SphK1 in DMS group, and the cells treated with equal amount of 0.9 % NaCl instead of drugs served as the control group. Tetradecanoylphorbol Acetate 71-102 sphingosine kinase 1 Homo sapiens 146-151 23541833-0 2013 Synthesis of (S)-FTY720 vinylphosphonate analogues and evaluation of their potential as sphingosine kinase 1 inhibitors and activators. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 13-40 sphingosine kinase 1 Homo sapiens 88-108 23541833-3 2013 In previous work, we synthesized a novel allosteric SK1 inhibitor, (S)-FTY720 vinylphosphonate. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 67-94 sphingosine kinase 1 Homo sapiens 52-55 23541833-4 2013 We now report a more expeditious route to this inhibitor which features B-alkyl Suzuki coupling as a key step and show that replacement of the amino group in (S)-FTY720 vinylphosphonate with an azido group converts the vinylphosphonate from an allosteric inhibitor to an activator of SK1 at low micromolar concentrations. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 158-185 sphingosine kinase 1 Homo sapiens 284-287 23541833-4 2013 We now report a more expeditious route to this inhibitor which features B-alkyl Suzuki coupling as a key step and show that replacement of the amino group in (S)-FTY720 vinylphosphonate with an azido group converts the vinylphosphonate from an allosteric inhibitor to an activator of SK1 at low micromolar concentrations. 3',5-diazido-2',3'-dideoxyuridine 194-199 sphingosine kinase 1 Homo sapiens 284-287 23541833-4 2013 We now report a more expeditious route to this inhibitor which features B-alkyl Suzuki coupling as a key step and show that replacement of the amino group in (S)-FTY720 vinylphosphonate with an azido group converts the vinylphosphonate from an allosteric inhibitor to an activator of SK1 at low micromolar concentrations. vinylphosphonate 169-185 sphingosine kinase 1 Homo sapiens 284-287 23541833-5 2013 Our results demonstrate the feasibility of using the (S)-FTY720 vinylphosphonate scaffold to define structure-activity relationships in the allosteric site of SK1. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 53-80 sphingosine kinase 1 Homo sapiens 159-162 24054007-2 2013 METHODS: Human colon cancer LoVo cells were divided into three groups: phorbol 12-myristate 13-acetate (PMA) was used to induce the activation of SphK1 in the PMA group, N,N-dimethylsphingosine (DMS) used to suppress the activity of SphK1 in DMS group, and the cells treated with equal amount of 0.9 % NaCl instead of drugs served as the control group. Tetradecanoylphorbol Acetate 71-102 sphingosine kinase 1 Homo sapiens 233-238 24054007-2 2013 METHODS: Human colon cancer LoVo cells were divided into three groups: phorbol 12-myristate 13-acetate (PMA) was used to induce the activation of SphK1 in the PMA group, N,N-dimethylsphingosine (DMS) used to suppress the activity of SphK1 in DMS group, and the cells treated with equal amount of 0.9 % NaCl instead of drugs served as the control group. Tetradecanoylphorbol Acetate 104-107 sphingosine kinase 1 Homo sapiens 146-151 23675553-1 2013 The sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) and its enzyme sphingosine kinase (SphK) play an important role in the regulation of cell proliferation, survival, inflammation, and cell death. Ceramides 29-37 sphingosine kinase 1 Homo sapiens 121-125 23675553-1 2013 The sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) and its enzyme sphingosine kinase (SphK) play an important role in the regulation of cell proliferation, survival, inflammation, and cell death. Sphingolipids 4-16 sphingosine kinase 1 Homo sapiens 101-119 23675553-1 2013 The sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) and its enzyme sphingosine kinase (SphK) play an important role in the regulation of cell proliferation, survival, inflammation, and cell death. Sphingolipids 4-16 sphingosine kinase 1 Homo sapiens 121-125 23675553-1 2013 The sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) and its enzyme sphingosine kinase (SphK) play an important role in the regulation of cell proliferation, survival, inflammation, and cell death. Ceramides 29-37 sphingosine kinase 1 Homo sapiens 101-119 23434415-1 2013 Sphingosine kinase (SPHK), which catalyzes the phosphorylation of sphingosine to generate sphingosine 1-phosphate, has two mammalian isotypes, SPHK1 and SPHK2. Sphingosine 66-77 sphingosine kinase 1 Homo sapiens 0-18 23434415-1 2013 Sphingosine kinase (SPHK), which catalyzes the phosphorylation of sphingosine to generate sphingosine 1-phosphate, has two mammalian isotypes, SPHK1 and SPHK2. Sphingosine 66-77 sphingosine kinase 1 Homo sapiens 20-24 23434415-1 2013 Sphingosine kinase (SPHK), which catalyzes the phosphorylation of sphingosine to generate sphingosine 1-phosphate, has two mammalian isotypes, SPHK1 and SPHK2. Sphingosine 66-77 sphingosine kinase 1 Homo sapiens 143-148 23434415-1 2013 Sphingosine kinase (SPHK), which catalyzes the phosphorylation of sphingosine to generate sphingosine 1-phosphate, has two mammalian isotypes, SPHK1 and SPHK2. sphingosine 1-phosphate 90-113 sphingosine kinase 1 Homo sapiens 0-18 23434415-1 2013 Sphingosine kinase (SPHK), which catalyzes the phosphorylation of sphingosine to generate sphingosine 1-phosphate, has two mammalian isotypes, SPHK1 and SPHK2. sphingosine 1-phosphate 90-113 sphingosine kinase 1 Homo sapiens 20-24 23434415-1 2013 Sphingosine kinase (SPHK), which catalyzes the phosphorylation of sphingosine to generate sphingosine 1-phosphate, has two mammalian isotypes, SPHK1 and SPHK2. sphingosine 1-phosphate 90-113 sphingosine kinase 1 Homo sapiens 143-148 23314175-1 2013 Two isoforms of sphingosine kinase, SK1 and SK2, catalyze the formation of the bioactive lipid sphingosine 1-phosphate (S1P) in mammalian cells. sphingosine 1-phosphate 95-118 sphingosine kinase 1 Homo sapiens 36-39 23314175-2 2013 We have previously shown that treatment of androgen-sensitive LNCaP prostate cancer cells with a non-selective SK isoform inhibitor, 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SKi), induces the proteasomal degradation of SK1. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 133-180 sphingosine kinase 1 Homo sapiens 227-230 23675553-1 2013 The sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) and its enzyme sphingosine kinase (SphK) play an important role in the regulation of cell proliferation, survival, inflammation, and cell death. Sphingosine 39-50 sphingosine kinase 1 Homo sapiens 101-119 23675553-1 2013 The sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) and its enzyme sphingosine kinase (SphK) play an important role in the regulation of cell proliferation, survival, inflammation, and cell death. Sphingosine 39-50 sphingosine kinase 1 Homo sapiens 121-125 23675553-1 2013 The sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) and its enzyme sphingosine kinase (SphK) play an important role in the regulation of cell proliferation, survival, inflammation, and cell death. sphingosine 1-phosphate 56-79 sphingosine kinase 1 Homo sapiens 101-119 23675553-1 2013 The sphingolipid metabolites ceramide, sphingosine, and sphingosine-1-phosphate (S1P) and its enzyme sphingosine kinase (SphK) play an important role in the regulation of cell proliferation, survival, inflammation, and cell death. sphingosine 1-phosphate 56-79 sphingosine kinase 1 Homo sapiens 121-125 23675553-5 2013 A central finding of these studies is that SphK is a critical regulator of the sphingolipid rheostat, as it not only produces the pro-growth, anti-apoptotic messenger S1P, but also decreases levels of pro-apoptotic ceramide and sphingosine. Sphingolipids 79-91 sphingosine kinase 1 Homo sapiens 43-47 23675553-5 2013 A central finding of these studies is that SphK is a critical regulator of the sphingolipid rheostat, as it not only produces the pro-growth, anti-apoptotic messenger S1P, but also decreases levels of pro-apoptotic ceramide and sphingosine. Ceramides 215-223 sphingosine kinase 1 Homo sapiens 43-47 23675553-5 2013 A central finding of these studies is that SphK is a critical regulator of the sphingolipid rheostat, as it not only produces the pro-growth, anti-apoptotic messenger S1P, but also decreases levels of pro-apoptotic ceramide and sphingosine. Sphingosine 228-239 sphingosine kinase 1 Homo sapiens 43-47 23388656-2 2013 We synthesized a series of sphingosine-based SK1-selective inhibitors, the most potent of which is RB-005 (IC(50) = 3.6 muM), which also induced proteasomal degradation of SK1 in human pulmonary arterial smooth muscle cells. Sphingosine 27-38 sphingosine kinase 1 Homo sapiens 45-48 23315259-5 2013 Also, the expression of SphK1 was increased in lung tissues from patients with IPF and bleomycin-challenged mice. Bleomycin 87-96 sphingosine kinase 1 Homo sapiens 24-29 23241891-2 2013 We show in this study that PGE(2), in combination with LPS, is able to promote an anti-inflammatory phenotype in macrophages characterized by high expression of IL-10 and the regulatory markers SPHK1 and LIGHT via a protein kinase A-dependent pathway. Prostaglandins E 27-30 sphingosine kinase 1 Homo sapiens 194-199 23388656-2 2013 We synthesized a series of sphingosine-based SK1-selective inhibitors, the most potent of which is RB-005 (IC(50) = 3.6 muM), which also induced proteasomal degradation of SK1 in human pulmonary arterial smooth muscle cells. Sphingosine 27-38 sphingosine kinase 1 Homo sapiens 172-175 23388656-2 2013 We synthesized a series of sphingosine-based SK1-selective inhibitors, the most potent of which is RB-005 (IC(50) = 3.6 muM), which also induced proteasomal degradation of SK1 in human pulmonary arterial smooth muscle cells. 1-(4-octylphenethyl)piperidin-4-ol 99-105 sphingosine kinase 1 Homo sapiens 45-48 23388656-2 2013 We synthesized a series of sphingosine-based SK1-selective inhibitors, the most potent of which is RB-005 (IC(50) = 3.6 muM), which also induced proteasomal degradation of SK1 in human pulmonary arterial smooth muscle cells. 1-(4-octylphenethyl)piperidin-4-ol 99-105 sphingosine kinase 1 Homo sapiens 172-175 23232649-5 2013 In addition, N,N-dimethylsphingosine (DMS), SphK1 DNA and shRNA transfection were used to regulate the expression and activity of SphK1 in the LOVO colon cancer cell line. N,N-dimethylsphingosine 13-36 sphingosine kinase 1 Homo sapiens 154-159 23232649-5 2013 In addition, N,N-dimethylsphingosine (DMS), SphK1 DNA and shRNA transfection were used to regulate the expression and activity of SphK1 in the LOVO colon cancer cell line. N,N-dimethylsphingosine 50-53 sphingosine kinase 1 Homo sapiens 154-159 23113536-3 2013 EXPERIMENTAL APPROACH: Androgen-sensitive LNCaP cells were treated with SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole), which inhibits sphingosine kinases 1 and 2 activity, and the effect on androgen receptor expression was measured. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 77-124 sphingosine kinase 1 Homo sapiens 142-169 23180720-8 2013 In the context of pressure-induced S1P signalling, the S1P-generating enzyme sphingosine kinase 1 serves as a divergence point, by orchestrating the calcium-dependent and -independent signalling pathways underlying microvascular myogenic responsiveness. Calcium 149-156 sphingosine kinase 1 Homo sapiens 77-97 22639116-1 2013 Sphingosine kinase 1 (SphK1) is an enzyme that phosphorylates the lipid sphingosine to generate sphingosine-1-phosphate (S1P). Sphingosine 72-83 sphingosine kinase 1 Homo sapiens 0-20 22639116-1 2013 Sphingosine kinase 1 (SphK1) is an enzyme that phosphorylates the lipid sphingosine to generate sphingosine-1-phosphate (S1P). Sphingosine 72-83 sphingosine kinase 1 Homo sapiens 22-27 22639116-1 2013 Sphingosine kinase 1 (SphK1) is an enzyme that phosphorylates the lipid sphingosine to generate sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 96-119 sphingosine kinase 1 Homo sapiens 0-20 22639116-1 2013 Sphingosine kinase 1 (SphK1) is an enzyme that phosphorylates the lipid sphingosine to generate sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 96-119 sphingosine kinase 1 Homo sapiens 22-27 23109673-6 2013 Mechanistically, SphK1 down-regulation by siRNA in human umbilical vein endothelial cells decreased cytokine-induced PGE(2) production primarily through inhibition of microsomal PGE synthase-1 (mPGES-1), not COX-2. Dinoprostone 117-123 sphingosine kinase 1 Homo sapiens 17-22 23166225-6 2013 SphK1 contribution to resistance was supported by the demonstration that SphK1 inhibition by N,N-dimethyl-sphingosine or silencing via siRNA in resistant cells restores sensitivity to cetuximab, whereas exogenous SphK1 overexpression in sensitive cells confers resistance to these agents. N,N-dimethylsphingosine 93-117 sphingosine kinase 1 Homo sapiens 73-78 23328479-1 2013 In this issue of Cancer Cell, Liang and colleagues demonstrated that sphingosine kinase 1, the enzyme that catalyzes formation of the biologically active lipid sphingosine 1-phosphate, drives a malicious amplification loop involving sphingosine 1-phosphate receptor 1 and the NF-kappaB/IL-6/STAT3 pathway. sphingosine 1-phosphate 160-183 sphingosine kinase 1 Homo sapiens 69-89 23290780-1 2013 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipids ceramide and sphingosine into the antiapoptotic lipid sphingosine-1-phosphate and activates the signal transduction pathways that lead to cell proliferation, migration, the activation of the inflammatory response, and the impairment of apoptosis. Ceramides 109-117 sphingosine kinase 1 Homo sapiens 0-20 23290780-1 2013 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipids ceramide and sphingosine into the antiapoptotic lipid sphingosine-1-phosphate and activates the signal transduction pathways that lead to cell proliferation, migration, the activation of the inflammatory response, and the impairment of apoptosis. Ceramides 109-117 sphingosine kinase 1 Homo sapiens 22-25 23290780-1 2013 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipids ceramide and sphingosine into the antiapoptotic lipid sphingosine-1-phosphate and activates the signal transduction pathways that lead to cell proliferation, migration, the activation of the inflammatory response, and the impairment of apoptosis. Sphingosine 122-133 sphingosine kinase 1 Homo sapiens 0-20 23290780-1 2013 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipids ceramide and sphingosine into the antiapoptotic lipid sphingosine-1-phosphate and activates the signal transduction pathways that lead to cell proliferation, migration, the activation of the inflammatory response, and the impairment of apoptosis. Sphingosine 122-133 sphingosine kinase 1 Homo sapiens 22-25 23290780-1 2013 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipids ceramide and sphingosine into the antiapoptotic lipid sphingosine-1-phosphate and activates the signal transduction pathways that lead to cell proliferation, migration, the activation of the inflammatory response, and the impairment of apoptosis. sphingosine 1-phosphate 163-186 sphingosine kinase 1 Homo sapiens 0-20 23290780-1 2013 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipids ceramide and sphingosine into the antiapoptotic lipid sphingosine-1-phosphate and activates the signal transduction pathways that lead to cell proliferation, migration, the activation of the inflammatory response, and the impairment of apoptosis. sphingosine 1-phosphate 163-186 sphingosine kinase 1 Homo sapiens 22-25 23290781-4 2013 While the relative contribution of SK2 is still being elucidated and may involve an intranuclear role, a substantial amount of evidence suggests that regulation of sphingolipid levels by SK1 is an important component of carcinogenesis. Sphingolipids 164-176 sphingosine kinase 1 Homo sapiens 187-190 23000541-1 2013 Sphingosine kinases 1 and 2 (SK1 and SK2) generate the bioactive lipid mediator sphingosine 1-phosphate and as such play a significant role in cell fate and in human health and disease. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 0-27 23000541-1 2013 Sphingosine kinases 1 and 2 (SK1 and SK2) generate the bioactive lipid mediator sphingosine 1-phosphate and as such play a significant role in cell fate and in human health and disease. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 29-32 23166225-2 2013 Alterations in the "sphingolipid rheostat"-the balance between the proapoptotic molecule ceramide and the mitogenic factor sphingosine-1-phosphate (S1P)-due to sphingosine kinase 1 (SphK1) overactivation have been involved in resistance to anticancer-targeted agents. Sphingolipids 20-32 sphingosine kinase 1 Homo sapiens 160-180 23166225-2 2013 Alterations in the "sphingolipid rheostat"-the balance between the proapoptotic molecule ceramide and the mitogenic factor sphingosine-1-phosphate (S1P)-due to sphingosine kinase 1 (SphK1) overactivation have been involved in resistance to anticancer-targeted agents. Sphingolipids 20-32 sphingosine kinase 1 Homo sapiens 182-187 23166225-2 2013 Alterations in the "sphingolipid rheostat"-the balance between the proapoptotic molecule ceramide and the mitogenic factor sphingosine-1-phosphate (S1P)-due to sphingosine kinase 1 (SphK1) overactivation have been involved in resistance to anticancer-targeted agents. Ceramides 89-97 sphingosine kinase 1 Homo sapiens 160-180 23166225-2 2013 Alterations in the "sphingolipid rheostat"-the balance between the proapoptotic molecule ceramide and the mitogenic factor sphingosine-1-phosphate (S1P)-due to sphingosine kinase 1 (SphK1) overactivation have been involved in resistance to anticancer-targeted agents. sphingosine 1-phosphate 123-146 sphingosine kinase 1 Homo sapiens 160-180 23166225-2 2013 Alterations in the "sphingolipid rheostat"-the balance between the proapoptotic molecule ceramide and the mitogenic factor sphingosine-1-phosphate (S1P)-due to sphingosine kinase 1 (SphK1) overactivation have been involved in resistance to anticancer-targeted agents. sphingosine 1-phosphate 148-151 sphingosine kinase 1 Homo sapiens 160-180 23166225-6 2013 SphK1 contribution to resistance was supported by the demonstration that SphK1 inhibition by N,N-dimethyl-sphingosine or silencing via siRNA in resistant cells restores sensitivity to cetuximab, whereas exogenous SphK1 overexpression in sensitive cells confers resistance to these agents. N,N-dimethylsphingosine 93-117 sphingosine kinase 1 Homo sapiens 0-5 23166225-6 2013 SphK1 contribution to resistance was supported by the demonstration that SphK1 inhibition by N,N-dimethyl-sphingosine or silencing via siRNA in resistant cells restores sensitivity to cetuximab, whereas exogenous SphK1 overexpression in sensitive cells confers resistance to these agents. N,N-dimethylsphingosine 93-117 sphingosine kinase 1 Homo sapiens 73-78 23085271-0 2012 Berberine suppresses high glucose-induced TGF-beta1 and fibronectin synthesis in mesangial cells through inhibition of sphingosine kinase 1/AP-1 pathway. Berberine 0-9 sphingosine kinase 1 Homo sapiens 119-139 23563649-1 2013 In this chapter, roles of bioactive sphingolipids, specifically sphingosine kinase 1 (SK1) and 2 (SK2) and their product-sphingosine 1-phosphate (S1P)-will be reviewed with respect to regulation of cancer growth, metastasis, chemotherapeutics, and drug resistance. Sphingolipids 36-49 sphingosine kinase 1 Homo sapiens 86-89 24396570-0 2013 Novel sphingosine-containing analogues selectively inhibit sphingosine kinase (SK) isozymes, induce SK1 proteasomal degradation and reduce DNA synthesis in human pulmonary arterial smooth muscle cells. Sphingosine 6-17 sphingosine kinase 1 Homo sapiens 100-103 24396570-3 2013 A thiourea adduct of sphinganine (F02) is selective for SK2 whereas the 1-deoxysphinganines 55-21 and 77-7 are selective for SK1. spisulosine 72-91 sphingosine kinase 1 Homo sapiens 125-128 24396570-4 2013 (2S,3R)-1-Deoxysphinganine (55-21) induced the proteasomal degradation of SK1 in human pulmonary arterial smooth muscle cells and inhibited DNA synthesis, while the more potent SK1 inhibitors PF-543 and VPC96091 failed to inhibit DNA synthesis. (2s,3r)-1-deoxysphinganine 0-26 sphingosine kinase 1 Homo sapiens 74-77 23818902-8 2013 Sphingosine kinase (SphK) is a ubiquitous enzyme responsible for the production of sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 83-106 sphingosine kinase 1 Homo sapiens 0-18 23818902-8 2013 Sphingosine kinase (SphK) is a ubiquitous enzyme responsible for the production of sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 83-106 sphingosine kinase 1 Homo sapiens 20-24 23085271-0 2012 Berberine suppresses high glucose-induced TGF-beta1 and fibronectin synthesis in mesangial cells through inhibition of sphingosine kinase 1/AP-1 pathway. Glucose 26-33 sphingosine kinase 1 Homo sapiens 119-139 23085271-2 2012 Recently, we found that berberine suppressed the activation of SphK1 pathway in diabetic kidney, protecting against diabetic nephropathy. Berberine 24-33 sphingosine kinase 1 Homo sapiens 63-68 23085271-5 2012 Furthermore, berberine suppressed the activation of SphK1 pathway via inhibition of the activity and expression of SphK1 in mesangial cells cultured by high glucose. Berberine 13-22 sphingosine kinase 1 Homo sapiens 52-57 23085271-5 2012 Furthermore, berberine suppressed the activation of SphK1 pathway via inhibition of the activity and expression of SphK1 in mesangial cells cultured by high glucose. Berberine 13-22 sphingosine kinase 1 Homo sapiens 115-120 23085271-5 2012 Furthermore, berberine suppressed the activation of SphK1 pathway via inhibition of the activity and expression of SphK1 in mesangial cells cultured by high glucose. Glucose 157-164 sphingosine kinase 1 Homo sapiens 52-57 23085271-5 2012 Furthermore, berberine suppressed the activation of SphK1 pathway via inhibition of the activity and expression of SphK1 in mesangial cells cultured by high glucose. Glucose 157-164 sphingosine kinase 1 Homo sapiens 115-120 23085271-6 2012 Surprisingly, berberine blocked the increased activity and expression of SphK1 in mesangial cells transfected by wild type SphK1 under normal glucose condition. Berberine 14-23 sphingosine kinase 1 Homo sapiens 73-78 23085271-6 2012 Surprisingly, berberine blocked the increased activity and expression of SphK1 in mesangial cells transfected by wild type SphK1 under normal glucose condition. Berberine 14-23 sphingosine kinase 1 Homo sapiens 123-128 23085271-6 2012 Surprisingly, berberine blocked the increased activity and expression of SphK1 in mesangial cells transfected by wild type SphK1 under normal glucose condition. Glucose 142-149 sphingosine kinase 1 Homo sapiens 73-78 23085271-9 2012 Altogether, these data not only demonstrate that berberine is an important agent against diabetic nephropathy through inhibition of SphK1/AP-1 pathway, but also indicate that the inhibition of SphK1/AP-1 by berberine is independent of ambient glucose concentration. Berberine 49-58 sphingosine kinase 1 Homo sapiens 132-137 23085271-9 2012 Altogether, these data not only demonstrate that berberine is an important agent against diabetic nephropathy through inhibition of SphK1/AP-1 pathway, but also indicate that the inhibition of SphK1/AP-1 by berberine is independent of ambient glucose concentration. Berberine 49-58 sphingosine kinase 1 Homo sapiens 193-198 23085271-9 2012 Altogether, these data not only demonstrate that berberine is an important agent against diabetic nephropathy through inhibition of SphK1/AP-1 pathway, but also indicate that the inhibition of SphK1/AP-1 by berberine is independent of ambient glucose concentration. Berberine 207-216 sphingosine kinase 1 Homo sapiens 193-198 23085271-9 2012 Altogether, these data not only demonstrate that berberine is an important agent against diabetic nephropathy through inhibition of SphK1/AP-1 pathway, but also indicate that the inhibition of SphK1/AP-1 by berberine is independent of ambient glucose concentration. Glucose 243-250 sphingosine kinase 1 Homo sapiens 193-198 23316473-1 2012 There is substantial evidence for a role in cancer of the bioactive lipid sphingosine 1-phosphate (S1P), the enzyme sphingosine kinase 1 (that catalyses S1P formation) and S1P-specific G protein-coupled receptors. sphingosine 1-phosphate 74-97 sphingosine kinase 1 Homo sapiens 116-136 22321213-1 2012 The conversion of sphingosine to sphingosine-1-phosphate is catalyzed by sphingosine kinase (SphK), which has been implicated in disease states such as cancer and fibrosis. Sphingosine 18-29 sphingosine kinase 1 Homo sapiens 73-91 22321213-1 2012 The conversion of sphingosine to sphingosine-1-phosphate is catalyzed by sphingosine kinase (SphK), which has been implicated in disease states such as cancer and fibrosis. Sphingosine 18-29 sphingosine kinase 1 Homo sapiens 93-97 22321213-1 2012 The conversion of sphingosine to sphingosine-1-phosphate is catalyzed by sphingosine kinase (SphK), which has been implicated in disease states such as cancer and fibrosis. sphingosine 1-phosphate 33-56 sphingosine kinase 1 Homo sapiens 73-91 22321213-1 2012 The conversion of sphingosine to sphingosine-1-phosphate is catalyzed by sphingosine kinase (SphK), which has been implicated in disease states such as cancer and fibrosis. sphingosine 1-phosphate 33-56 sphingosine kinase 1 Homo sapiens 93-97 22321213-4 2012 Building on our initial work that focused on the structure-activity relationship study on an FTY720-derived cylohexylamine scaffold, we report that varying the alkyl chain length on the hydrophobic tail can impart selectivity toward SphK2 over SphK1. Cyclohexylamines 108-122 sphingosine kinase 1 Homo sapiens 244-249 22833671-0 2012 Oncogenic K-Ras regulates bioactive sphingolipids in a sphingosine kinase 1-dependent manner. Sphingolipids 36-49 sphingosine kinase 1 Homo sapiens 55-75 22922818-8 2012 Our data show that, upstream of calcium, inhibition of PI3K and Sphk activity decreases inducible IL-33 expression to IgE/Ag activation. Calcium 32-39 sphingosine kinase 1 Homo sapiens 64-68 22833671-8 2012 We believe this research has important implications for how sphingolipids may be contributing to oncogenic transformation and provide some of the first evidence for oncogenes inducing specific changes in sphingolipid metabolism through SK1 regulation. Sphingolipids 60-73 sphingosine kinase 1 Homo sapiens 236-239 22833671-8 2012 We believe this research has important implications for how sphingolipids may be contributing to oncogenic transformation and provide some of the first evidence for oncogenes inducing specific changes in sphingolipid metabolism through SK1 regulation. Sphingolipids 60-72 sphingosine kinase 1 Homo sapiens 236-239 22684547-11 2012 Moreover, SphK1 was required for the production of MMP-2/9 and uPA in tumor cells, which was suppressed by ERK1/2 inhibitor U0126, but enhanced by the p38 MAPK inhibitor SB203580. U 0126 124-129 sphingosine kinase 1 Homo sapiens 10-15 22684547-11 2012 Moreover, SphK1 was required for the production of MMP-2/9 and uPA in tumor cells, which was suppressed by ERK1/2 inhibitor U0126, but enhanced by the p38 MAPK inhibitor SB203580. SB 203580 170-178 sphingosine kinase 1 Homo sapiens 10-15 22833671-1 2012 Sphingosine kinase 1 (SK1) is an important enzyme involved in the production of the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 100-123 sphingosine kinase 1 Homo sapiens 0-20 22727936-6 2012 The sphingosine kinase 1 (SK1) inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SK1-II) sensitized the cells to the cytotoxic effects of selenite. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 41-88 sphingosine kinase 1 Homo sapiens 26-29 22727936-6 2012 The sphingosine kinase 1 (SK1) inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SK1-II) sensitized the cells to the cytotoxic effects of selenite. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 41-88 sphingosine kinase 1 Homo sapiens 90-93 22833671-1 2012 Sphingosine kinase 1 (SK1) is an important enzyme involved in the production of the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 100-123 sphingosine kinase 1 Homo sapiens 22-25 22727936-6 2012 The sphingosine kinase 1 (SK1) inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SK1-II) sensitized the cells to the cytotoxic effects of selenite. Selenious Acid 147-155 sphingosine kinase 1 Homo sapiens 4-24 22634604-1 2012 Sphingosine kinase 1 catalyses the formation of the bioactive lipid, sphingosine 1-phosphate and is a target for anti-cancer agents. sphingosine 1-phosphate 69-92 sphingosine kinase 1 Homo sapiens 0-20 22727936-6 2012 The sphingosine kinase 1 (SK1) inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SK1-II) sensitized the cells to the cytotoxic effects of selenite. Selenious Acid 147-155 sphingosine kinase 1 Homo sapiens 26-29 22727936-6 2012 The sphingosine kinase 1 (SK1) inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SK1-II) sensitized the cells to the cytotoxic effects of selenite. Selenious Acid 147-155 sphingosine kinase 1 Homo sapiens 90-93 22727936-7 2012 Preincubation with 10 muM of SK1-II prior to treatment with 10 muM of selenite caused induction of apoptosis and gave rise to a 2.5-fold increase in C14-, C16-, C18- and C18:1- ceramides. Selenious Acid 70-78 sphingosine kinase 1 Homo sapiens 29-32 22727936-7 2012 Preincubation with 10 muM of SK1-II prior to treatment with 10 muM of selenite caused induction of apoptosis and gave rise to a 2.5-fold increase in C14-, C16-, C18- and C18:1- ceramides. 1- ceramides 174-186 sphingosine kinase 1 Homo sapiens 29-32 22727936-8 2012 Instead, 50 muM of SK1-II combined with 10 muM of selenite caused accumulation of cells in G1/S phases, but less apoptosis and accumulation of ceramides. Ceramides 143-152 sphingosine kinase 1 Homo sapiens 19-22 22727936-9 2012 The formation of reactive oxygen species (ROS) after treatment with 10 muM of selenite was maximally enhanced by 1 muM of SK1-II. Reactive Oxygen Species 17-40 sphingosine kinase 1 Homo sapiens 122-125 22727936-9 2012 The formation of reactive oxygen species (ROS) after treatment with 10 muM of selenite was maximally enhanced by 1 muM of SK1-II. Reactive Oxygen Species 42-45 sphingosine kinase 1 Homo sapiens 122-125 22727936-9 2012 The formation of reactive oxygen species (ROS) after treatment with 10 muM of selenite was maximally enhanced by 1 muM of SK1-II. Selenious Acid 78-86 sphingosine kinase 1 Homo sapiens 122-125 22634604-2 2012 We demonstrate here that 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SKi, also referred to as SKI-II), FTY720 (Fingolimod), and (S)-FTY720 vinylphosphonate inhibit sphingosine kinase 1 activity with distinct kinetics, indicating that these compounds exhibit different binding modalities with sphingosine kinase 1. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 25-72 sphingosine kinase 1 Homo sapiens 169-189 22634604-2 2012 We demonstrate here that 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SKi, also referred to as SKI-II), FTY720 (Fingolimod), and (S)-FTY720 vinylphosphonate inhibit sphingosine kinase 1 activity with distinct kinetics, indicating that these compounds exhibit different binding modalities with sphingosine kinase 1. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 25-72 sphingosine kinase 1 Homo sapiens 297-317 22594559-0 2012 Sphingosine kinase-1 inhibition sensitizes curcumin-induced growth inhibition and apoptosis in ovarian cancer cells. Curcumin 43-51 sphingosine kinase 1 Homo sapiens 0-20 22594559-4 2012 Inhibition of SphK1 by SKI-II or by RNA interference (RNAi) knockdown dramatically enhanced curcumin-induced apoptosis and growth inhibition in ovarian cancer cells. Curcumin 92-100 sphingosine kinase 1 Homo sapiens 14-19 22727936-0 2012 Inhibition of sphingosine kinase 1 enhances cytotoxicity, ceramide levels and ROS formation in liver cancer cells treated with selenite. Ceramides 58-66 sphingosine kinase 1 Homo sapiens 14-34 22727936-0 2012 Inhibition of sphingosine kinase 1 enhances cytotoxicity, ceramide levels and ROS formation in liver cancer cells treated with selenite. Reactive Oxygen Species 78-81 sphingosine kinase 1 Homo sapiens 14-34 22727936-0 2012 Inhibition of sphingosine kinase 1 enhances cytotoxicity, ceramide levels and ROS formation in liver cancer cells treated with selenite. Selenious Acid 127-135 sphingosine kinase 1 Homo sapiens 14-34 22727936-6 2012 The sphingosine kinase 1 (SK1) inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole (SK1-II) sensitized the cells to the cytotoxic effects of selenite. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 41-88 sphingosine kinase 1 Homo sapiens 4-24 22583777-0 2012 Co-administration phenoxodiol with doxorubicin synergistically inhibit the activity of sphingosine kinase-1 (SphK1), a potential oncogene of osteosarcoma, to suppress osteosarcoma cell growth both in vivo and in vitro. phenoxodiol 18-29 sphingosine kinase 1 Homo sapiens 87-107 22583777-0 2012 Co-administration phenoxodiol with doxorubicin synergistically inhibit the activity of sphingosine kinase-1 (SphK1), a potential oncogene of osteosarcoma, to suppress osteosarcoma cell growth both in vivo and in vitro. phenoxodiol 18-29 sphingosine kinase 1 Homo sapiens 109-114 22583777-0 2012 Co-administration phenoxodiol with doxorubicin synergistically inhibit the activity of sphingosine kinase-1 (SphK1), a potential oncogene of osteosarcoma, to suppress osteosarcoma cell growth both in vivo and in vitro. Doxorubicin 35-46 sphingosine kinase 1 Homo sapiens 87-107 22583777-0 2012 Co-administration phenoxodiol with doxorubicin synergistically inhibit the activity of sphingosine kinase-1 (SphK1), a potential oncogene of osteosarcoma, to suppress osteosarcoma cell growth both in vivo and in vitro. Doxorubicin 35-46 sphingosine kinase 1 Homo sapiens 109-114 22583777-4 2012 Over-expression of SphK1 in OS cell line U2OS promoted its growth and endorsed its resistance against doxorubicin, while knocking-down of SphK1 by shRNA inhibited U2OS cell growth and increased its sensitivity to doxorubicin. Doxorubicin 102-113 sphingosine kinase 1 Homo sapiens 19-24 22583777-4 2012 Over-expression of SphK1 in OS cell line U2OS promoted its growth and endorsed its resistance against doxorubicin, while knocking-down of SphK1 by shRNA inhibited U2OS cell growth and increased its sensitivity to doxorubicin. Doxorubicin 213-224 sphingosine kinase 1 Homo sapiens 19-24 22583777-4 2012 Over-expression of SphK1 in OS cell line U2OS promoted its growth and endorsed its resistance against doxorubicin, while knocking-down of SphK1 by shRNA inhibited U2OS cell growth and increased its sensitivity to doxorubicin. Doxorubicin 213-224 sphingosine kinase 1 Homo sapiens 138-143 22583777-5 2012 Co-administration phenoxodiol with doxorubicin synergistically inhibited SphK1 activity to trigger cellular ceramide accumulation, and achieved synergistic anti-OS growth effect, accompanied with a significant increased of apoptosis and cytotoxicity. phenoxodiol 18-29 sphingosine kinase 1 Homo sapiens 73-78 22583777-5 2012 Co-administration phenoxodiol with doxorubicin synergistically inhibited SphK1 activity to trigger cellular ceramide accumulation, and achieved synergistic anti-OS growth effect, accompanied with a significant increased of apoptosis and cytotoxicity. Doxorubicin 35-46 sphingosine kinase 1 Homo sapiens 73-78 22583777-5 2012 Co-administration phenoxodiol with doxorubicin synergistically inhibited SphK1 activity to trigger cellular ceramide accumulation, and achieved synergistic anti-OS growth effect, accompanied with a significant increased of apoptosis and cytotoxicity. Ceramides 108-116 sphingosine kinase 1 Homo sapiens 73-78 22583777-9 2012 In conclusion, our data suggest that SphK1 might be a critical oncogene of OS and co-administration phenoxodiol with doxorubicin synergistically inhibited the activity of SphK1 to suppress osteosarcoma cell growth both in vivo and in vitro. phenoxodiol 100-111 sphingosine kinase 1 Homo sapiens 171-176 22583777-9 2012 In conclusion, our data suggest that SphK1 might be a critical oncogene of OS and co-administration phenoxodiol with doxorubicin synergistically inhibited the activity of SphK1 to suppress osteosarcoma cell growth both in vivo and in vitro. Doxorubicin 117-128 sphingosine kinase 1 Homo sapiens 171-176 22251058-0 2012 Resveratrol dimers are novel sphingosine kinase 1 inhibitors and affect sphingosine kinase 1 expression and cancer cell growth and survival. Resveratrol 0-11 sphingosine kinase 1 Homo sapiens 29-49 22251058-0 2012 Resveratrol dimers are novel sphingosine kinase 1 inhibitors and affect sphingosine kinase 1 expression and cancer cell growth and survival. Resveratrol 0-11 sphingosine kinase 1 Homo sapiens 72-92 22251058-1 2012 BACKGROUND AND PURPOSE: Sphingosine kinase 1 catalyses formation of the bioactive lipid, sphingosine 1-phosphate, which protects cancer cells from apoptosis. sphingosine 1-phosphate 89-112 sphingosine kinase 1 Homo sapiens 24-44 22251058-3 2012 We have assessed the effect of the anti-cancer agent, resveratrol and its dimers (ampelopsin A and balanocarpol) on sphingosine kinase 1 activity and on survival of MCF-7 breast cancer cells. Resveratrol 54-65 sphingosine kinase 1 Homo sapiens 116-136 22251058-3 2012 We have assessed the effect of the anti-cancer agent, resveratrol and its dimers (ampelopsin A and balanocarpol) on sphingosine kinase 1 activity and on survival of MCF-7 breast cancer cells. Balanocarpol 99-111 sphingosine kinase 1 Homo sapiens 116-136 22251058-5 2012 KEY RESULTS: Resveratrol, ampelopsin A and balanocarpol were novel inhibitors of sphingosine kinase 1 activity. Resveratrol 13-24 sphingosine kinase 1 Homo sapiens 81-101 22251058-6 2012 Balanocarpol was a mixed inhibitor (with sphingosine) of sphingosine kinase 1 with a K(ic) = 90 +- 10 microM and a K(iu) of ~500 microM. Balanocarpol 0-12 sphingosine kinase 1 Homo sapiens 57-77 22251058-6 2012 Balanocarpol was a mixed inhibitor (with sphingosine) of sphingosine kinase 1 with a K(ic) = 90 +- 10 microM and a K(iu) of ~500 microM. Sphingosine 41-52 sphingosine kinase 1 Homo sapiens 57-77 22251058-8 2012 Resveratrol was a competitive inhibitor (with sphingosine) of sphingosine kinase 1 with a K(ic) = 160 +- 40 microM, reduced sphingosine kinase 1 expression and induced PARP cleavage in MCF-7 cells. Resveratrol 0-11 sphingosine kinase 1 Homo sapiens 62-82 22251058-8 2012 Resveratrol was a competitive inhibitor (with sphingosine) of sphingosine kinase 1 with a K(ic) = 160 +- 40 microM, reduced sphingosine kinase 1 expression and induced PARP cleavage in MCF-7 cells. Resveratrol 0-11 sphingosine kinase 1 Homo sapiens 124-144 22251058-8 2012 Resveratrol was a competitive inhibitor (with sphingosine) of sphingosine kinase 1 with a K(ic) = 160 +- 40 microM, reduced sphingosine kinase 1 expression and induced PARP cleavage in MCF-7 cells. Sphingosine 46-57 sphingosine kinase 1 Homo sapiens 62-82 22251058-9 2012 CONCLUSIONS AND IMPLICATIONS: Each molecule of balanocarpol may bind at least two sphingosine kinase 1 catalytic molecules to reduce the activity of each simultaneously. Balanocarpol 47-59 sphingosine kinase 1 Homo sapiens 82-102 22251058-10 2012 These findings suggest that resveratrol, ampelopsin A and balanocarpol could perturb sphingosine kinase 1-mediated signalling and this might explain their activity against MCF-7 breast cancer cells. Resveratrol 28-39 sphingosine kinase 1 Homo sapiens 85-105 22335554-5 2012 report that resveratrol and its higher order oligomers inhibit sphingosine kinase 1 (SphK1). Resveratrol 12-23 sphingosine kinase 1 Homo sapiens 63-83 22335554-5 2012 report that resveratrol and its higher order oligomers inhibit sphingosine kinase 1 (SphK1). Resveratrol 12-23 sphingosine kinase 1 Homo sapiens 85-90 22335554-6 2012 SphK1 is a key regulator of sphingolipid metabolism and alterations of this key metabolic pathway have been linked to many hyperproliferative diseases. Sphingolipids 28-40 sphingosine kinase 1 Homo sapiens 0-5 22335554-7 2012 This study identifies a target for the action of resveratrol and its higher order oligomers and opens the door to evaluation of SphK1 as a target for chemo-prevention of cancer. Resveratrol 49-60 sphingosine kinase 1 Homo sapiens 128-133 22594559-2 2012 Using in vitro approaches, we analyzed the impact of sphingosine kinase-1 (SphK-1) inhibition on ceramide production, and evaluated SphK1 inhibitor II (SKI-II) as a potential curcumin chemo-sensitizer in ovarian cancer cells. Ceramides 97-105 sphingosine kinase 1 Homo sapiens 75-81 22594559-2 2012 Using in vitro approaches, we analyzed the impact of sphingosine kinase-1 (SphK-1) inhibition on ceramide production, and evaluated SphK1 inhibitor II (SKI-II) as a potential curcumin chemo-sensitizer in ovarian cancer cells. Curcumin 175-183 sphingosine kinase 1 Homo sapiens 132-137 22397330-1 2012 SphK (sphingosine kinase) is the major source of the bioactive lipid and GPCR (G-protein-coupled receptor) agonist S1P (sphingosine 1-phosphate). sphingosine 1-phosphate 120-143 sphingosine kinase 1 Homo sapiens 0-4 22469881-2 2012 Sphingosine kinase (Sphk), the primary enzyme metabolizing the sphingolipid ceramide into sphingosine-1-phosphate (S1P), is a primary mediator of breast cancer promotion, survival and chemoresistance. sphingolipid ceramide 75-96 sphingosine kinase 1 Homo sapiens 0-18 22469881-2 2012 Sphingosine kinase (Sphk), the primary enzyme metabolizing the sphingolipid ceramide into sphingosine-1-phosphate (S1P), is a primary mediator of breast cancer promotion, survival and chemoresistance. sphingolipid ceramide 75-96 sphingosine kinase 1 Homo sapiens 32-36 22469881-2 2012 Sphingosine kinase (Sphk), the primary enzyme metabolizing the sphingolipid ceramide into sphingosine-1-phosphate (S1P), is a primary mediator of breast cancer promotion, survival and chemoresistance. sphingosine 1-phosphate 102-125 sphingosine kinase 1 Homo sapiens 0-18 22469881-2 2012 Sphingosine kinase (Sphk), the primary enzyme metabolizing the sphingolipid ceramide into sphingosine-1-phosphate (S1P), is a primary mediator of breast cancer promotion, survival and chemoresistance. sphingosine 1-phosphate 102-125 sphingosine kinase 1 Homo sapiens 32-36 22469881-2 2012 Sphingosine kinase (Sphk), the primary enzyme metabolizing the sphingolipid ceramide into sphingosine-1-phosphate (S1P), is a primary mediator of breast cancer promotion, survival and chemoresistance. sphingosine 1-phosphate 139-142 sphingosine kinase 1 Homo sapiens 0-18 22469881-2 2012 Sphingosine kinase (Sphk), the primary enzyme metabolizing the sphingolipid ceramide into sphingosine-1-phosphate (S1P), is a primary mediator of breast cancer promotion, survival and chemoresistance. sphingosine 1-phosphate 139-142 sphingosine kinase 1 Homo sapiens 32-36 22397330-1 2012 SphK (sphingosine kinase) is the major source of the bioactive lipid and GPCR (G-protein-coupled receptor) agonist S1P (sphingosine 1-phosphate). sphingosine 1-phosphate 120-143 sphingosine kinase 1 Homo sapiens 6-24 22397330-5 2012 PF-543 inhibits SphK1 with a K(i) of 3.6 nM, is sphingosine-competitive and is more than 100-fold selective for SphK1 over the SphK2 isoform. PF-543 0-6 sphingosine kinase 1 Homo sapiens 16-21 22397330-5 2012 PF-543 inhibits SphK1 with a K(i) of 3.6 nM, is sphingosine-competitive and is more than 100-fold selective for SphK1 over the SphK2 isoform. PF-543 0-6 sphingosine kinase 1 Homo sapiens 112-117 22397330-8 2012 PF-543 was effective as a potent inhibitor of S1P formation in whole blood, indicating that the SphK1 isoform of sphingosine kinase is the major source of S1P in human blood. PF-543 0-6 sphingosine kinase 1 Homo sapiens 96-101 22397330-8 2012 PF-543 was effective as a potent inhibitor of S1P formation in whole blood, indicating that the SphK1 isoform of sphingosine kinase is the major source of S1P in human blood. PF-543 0-6 sphingosine kinase 1 Homo sapiens 113-131 22397330-9 2012 PF-543 is the most potent inhibitor of SphK1 described to date and it will be useful for dissecting specific roles of SphK1-driven S1P signalling. PF-543 0-6 sphingosine kinase 1 Homo sapiens 39-44 22397330-9 2012 PF-543 is the most potent inhibitor of SphK1 described to date and it will be useful for dissecting specific roles of SphK1-driven S1P signalling. PF-543 0-6 sphingosine kinase 1 Homo sapiens 118-123 22422617-6 2012 Treatment with LDL promoted activation and translocation of endogenous sphingosine kinase 1 (SK1) from the cytosol to the plasma membrane concomitant with production of sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 169-192 sphingosine kinase 1 Homo sapiens 71-91 22533672-3 2012 S1P is synthesized from sphingosine by two SphKs (sphingosine kinases) (SphK1 and SphK2). Sphingosine 24-35 sphingosine kinase 1 Homo sapiens 72-77 22422617-6 2012 Treatment with LDL promoted activation and translocation of endogenous sphingosine kinase 1 (SK1) from the cytosol to the plasma membrane concomitant with production of sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 194-197 sphingosine kinase 1 Homo sapiens 71-91 22422617-7 2012 Pretreating cells with SK inhibitor, dimethylsphinogsine or down-regulation of SK1 and SK2 revealed that LDL-dependent activation of ERK1/2 and JNK is mediated by SK1. dimethylsphinogsine 37-56 sphingosine kinase 1 Homo sapiens 163-166 22422617-9 2012 Pretreating cells with S1P1/S1P3 receptor antagonist VPC23019 significantly inhibited activation of ERK1/2 and JNK by LDL, suggesting that LDL elicits G protein-dependent activation of ERK1/2 and JNK by stimulating SK1-dependent transactivation of S1P receptors. VPC23019 53-61 sphingosine kinase 1 Homo sapiens 215-218 22422617-11 2012 LDL-induced CTGF expression was pertussis toxin sensitive and inhibited by dimethylsphinogsine down-regulation of SK1 and VPC23019 treatment. dimethylsphinogsine 75-94 sphingosine kinase 1 Homo sapiens 114-117 22460268-7 2012 Treatment of MDA-MB-453 cells with the SK1 inhibitor, SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole) reduced the basal and S1P/S1P(4)-induced activation of ERK-1/2 and altered HER2 trafficking in these cells. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 59-106 sphingosine kinase 1 Homo sapiens 39-42 22277942-3 2012 We show here that sphingosine levels increase and sphingosine kinase (SPHK1) expression decreases after inactivating SERCA2b with the specific SERCA2 inhibitors thapsigargin (TG) or small interfering RNA to SERCA2b. Thapsigargin 161-173 sphingosine kinase 1 Homo sapiens 70-75 22288844-1 2012 OBJECTIVES: Sphingosine kinase (SphK), which is regulated by hypoxia, catalyses phosphorylation of sphingosine to produce sphingosine-1-phosphate, which stimulates invasiveness of gliomas. Sphingosine 99-110 sphingosine kinase 1 Homo sapiens 12-30 22288844-1 2012 OBJECTIVES: Sphingosine kinase (SphK), which is regulated by hypoxia, catalyses phosphorylation of sphingosine to produce sphingosine-1-phosphate, which stimulates invasiveness of gliomas. Sphingosine 99-110 sphingosine kinase 1 Homo sapiens 32-36 22288844-1 2012 OBJECTIVES: Sphingosine kinase (SphK), which is regulated by hypoxia, catalyses phosphorylation of sphingosine to produce sphingosine-1-phosphate, which stimulates invasiveness of gliomas. sphingosine 1-phosphate 122-145 sphingosine kinase 1 Homo sapiens 12-30 22288844-1 2012 OBJECTIVES: Sphingosine kinase (SphK), which is regulated by hypoxia, catalyses phosphorylation of sphingosine to produce sphingosine-1-phosphate, which stimulates invasiveness of gliomas. sphingosine 1-phosphate 122-145 sphingosine kinase 1 Homo sapiens 32-36 22236408-3 2012 This study shows that elevated sphingosine-1-phosphate (S-1-P) levels resulting from increased activity of sphingosine kinase-1 (SPHK1) occur in advanced melanomas. sphingosine 1-phosphate 56-61 sphingosine kinase 1 Homo sapiens 129-134 22530637-2 2012 Sphingosine kinase (SphK) phosphorylates sphingosine into sphingosine-1-phosphate. Sphingosine 41-52 sphingosine kinase 1 Homo sapiens 0-18 22530637-2 2012 Sphingosine kinase (SphK) phosphorylates sphingosine into sphingosine-1-phosphate. Sphingosine 41-52 sphingosine kinase 1 Homo sapiens 20-24 22530637-2 2012 Sphingosine kinase (SphK) phosphorylates sphingosine into sphingosine-1-phosphate. sphingosine 1-phosphate 58-81 sphingosine kinase 1 Homo sapiens 0-18 22530637-2 2012 Sphingosine kinase (SphK) phosphorylates sphingosine into sphingosine-1-phosphate. sphingosine 1-phosphate 58-81 sphingosine kinase 1 Homo sapiens 20-24 22098666-1 2012 BACKGROUND/AIMS: Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers, and may act as an oncogene in tumorigenesis. Sphingosine 68-79 sphingosine kinase 1 Homo sapiens 17-37 22098666-1 2012 BACKGROUND/AIMS: Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers, and may act as an oncogene in tumorigenesis. Sphingosine 68-79 sphingosine kinase 1 Homo sapiens 39-44 22098666-1 2012 BACKGROUND/AIMS: Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers, and may act as an oncogene in tumorigenesis. sphingosine 1-phosphate 83-106 sphingosine kinase 1 Homo sapiens 17-37 22098666-1 2012 BACKGROUND/AIMS: Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers, and may act as an oncogene in tumorigenesis. sphingosine 1-phosphate 83-106 sphingosine kinase 1 Homo sapiens 39-44 22780925-1 2012 OBJECTIVE: To study the effect of the sphingosine kinase 1 (SphK1) inhibitor N,N-dimethylsphingosine (DMS) in combination with chemotherapeutic drugs (DDP, 5-Fu, MMC) on the proliferation of gastric cancer cells (SGC7901) in vitro, and to evaluate whether SphK1 inhibitors could be used as synergetic agents in chemotherapy. N,N-dimethylsphingosine 77-100 sphingosine kinase 1 Homo sapiens 38-58 22780925-1 2012 OBJECTIVE: To study the effect of the sphingosine kinase 1 (SphK1) inhibitor N,N-dimethylsphingosine (DMS) in combination with chemotherapeutic drugs (DDP, 5-Fu, MMC) on the proliferation of gastric cancer cells (SGC7901) in vitro, and to evaluate whether SphK1 inhibitors could be used as synergetic agents in chemotherapy. N,N-dimethylsphingosine 77-100 sphingosine kinase 1 Homo sapiens 60-65 22780925-1 2012 OBJECTIVE: To study the effect of the sphingosine kinase 1 (SphK1) inhibitor N,N-dimethylsphingosine (DMS) in combination with chemotherapeutic drugs (DDP, 5-Fu, MMC) on the proliferation of gastric cancer cells (SGC7901) in vitro, and to evaluate whether SphK1 inhibitors could be used as synergetic agents in chemotherapy. N,N-dimethylsphingosine 77-100 sphingosine kinase 1 Homo sapiens 256-261 22780925-1 2012 OBJECTIVE: To study the effect of the sphingosine kinase 1 (SphK1) inhibitor N,N-dimethylsphingosine (DMS) in combination with chemotherapeutic drugs (DDP, 5-Fu, MMC) on the proliferation of gastric cancer cells (SGC7901) in vitro, and to evaluate whether SphK1 inhibitors could be used as synergetic agents in chemotherapy. N,N-dimethylsphingosine 102-105 sphingosine kinase 1 Homo sapiens 38-58 22780925-1 2012 OBJECTIVE: To study the effect of the sphingosine kinase 1 (SphK1) inhibitor N,N-dimethylsphingosine (DMS) in combination with chemotherapeutic drugs (DDP, 5-Fu, MMC) on the proliferation of gastric cancer cells (SGC7901) in vitro, and to evaluate whether SphK1 inhibitors could be used as synergetic agents in chemotherapy. N,N-dimethylsphingosine 102-105 sphingosine kinase 1 Homo sapiens 60-65 22326625-3 2012 More importantly, RT-PCR results revealed that expression levels of glucosyleceramide synthase and sphingosine kinase-1 were downregulated and ceramide synthase genes were upregulated in response to docetaxel. Docetaxel 199-208 sphingosine kinase 1 Homo sapiens 99-119 22236408-3 2012 This study shows that elevated sphingosine-1-phosphate (S-1-P) levels resulting from increased activity of sphingosine kinase-1 (SPHK1) occur in advanced melanomas. sphingosine 1-phosphate 31-54 sphingosine kinase 1 Homo sapiens 107-127 22236408-3 2012 This study shows that elevated sphingosine-1-phosphate (S-1-P) levels resulting from increased activity of sphingosine kinase-1 (SPHK1) occur in advanced melanomas. sphingosine 1-phosphate 31-54 sphingosine kinase 1 Homo sapiens 129-134 22236408-3 2012 This study shows that elevated sphingosine-1-phosphate (S-1-P) levels resulting from increased activity of sphingosine kinase-1 (SPHK1) occur in advanced melanomas. sphingosine 1-phosphate 56-61 sphingosine kinase 1 Homo sapiens 107-127 22298596-0 2012 Sphingosine-1-phosphate produced by sphingosine kinase 1 promotes breast cancer progression by stimulating angiogenesis and lymphangiogenesis. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 36-56 22227192-1 2012 Sphingosine kinase 1 (SPK1) is a key enzyme in the sphingolipid metabolic pathway. Sphingolipids 51-63 sphingosine kinase 1 Homo sapiens 0-20 22227192-1 2012 Sphingosine kinase 1 (SPK1) is a key enzyme in the sphingolipid metabolic pathway. Sphingolipids 51-63 sphingosine kinase 1 Homo sapiens 22-26 22227192-3 2012 Here, we present evidence that SPK1 is acetylated by the intrinsic acetyltransferase activity of p300/cAMP-response element-binding protein (CREB)-binding protein (CBP) at a conserved acetylation motif (the GK motif). Cyclic AMP 102-106 sphingosine kinase 1 Homo sapiens 31-35 22227192-5 2012 Mutation of two lysine (K) residues in its GK motif to either arginine (R) or glutamine (Q) blocked SPK1 ubiquitination and prevented its degradation by the proteasome. Lysine 16-22 sphingosine kinase 1 Homo sapiens 100-104 22227192-5 2012 Mutation of two lysine (K) residues in its GK motif to either arginine (R) or glutamine (Q) blocked SPK1 ubiquitination and prevented its degradation by the proteasome. Arginine 62-70 sphingosine kinase 1 Homo sapiens 100-104 22227192-5 2012 Mutation of two lysine (K) residues in its GK motif to either arginine (R) or glutamine (Q) blocked SPK1 ubiquitination and prevented its degradation by the proteasome. Glutamine 78-87 sphingosine kinase 1 Homo sapiens 100-104 22227192-6 2012 The processes of acetylation and ubiquitination may compete for the same lysine residues and, therefore, form a switch for SPK1 protein regulation. Lysine 73-79 sphingosine kinase 1 Homo sapiens 123-127 22236408-5 2012 Pharmacological SPHK1 inhibitors SKI-I but not SKI-II decreased S-1-P content, elevated ceramide levels, caused a G2-M block and induced apoptotic cell death in melanomas. dexbrompheniramine 66-69 sphingosine kinase 1 Homo sapiens 16-21 22236408-5 2012 Pharmacological SPHK1 inhibitors SKI-I but not SKI-II decreased S-1-P content, elevated ceramide levels, caused a G2-M block and induced apoptotic cell death in melanomas. Ceramides 88-96 sphingosine kinase 1 Homo sapiens 16-21 22524836-8 2012 All these data indicated that SKI-II could reverse drug resistance of SGC7901/DDP to cisplatin by down-regulating expression of P-gp and up-regulating apoptosis through down-regulation of SphK1. Cisplatin 85-94 sphingosine kinase 1 Homo sapiens 188-193 22312298-0 2012 Isoflurane protects against human endothelial cell apoptosis by inducing sphingosine kinase-1 via ERK MAPK. Isoflurane 0-10 sphingosine kinase 1 Homo sapiens 73-106 22137932-2 2012 SphK phosphorylates sphingosine to form sphingosine 1-phosphate (S1P), which has been implicated in cancer growth and survival. Sphingosine 20-31 sphingosine kinase 1 Homo sapiens 0-4 22137932-2 2012 SphK phosphorylates sphingosine to form sphingosine 1-phosphate (S1P), which has been implicated in cancer growth and survival. sphingosine 1-phosphate 40-63 sphingosine kinase 1 Homo sapiens 0-4 22137932-8 2012 This is the first report describing quaternary ammonium salts as SphK inhibitors. quaternary ammonium salts 36-61 sphingosine kinase 1 Homo sapiens 65-69 21857502-0 2012 Methyl-beta-cyclodextrin induces programmed cell death in chronic myeloid leukemia cells and, combined with imatinib, produces a synergistic downregulation of ERK/SPK1 signaling. methyl-beta-cyclodextrin 0-24 sphingosine kinase 1 Homo sapiens 163-167 21857502-0 2012 Methyl-beta-cyclodextrin induces programmed cell death in chronic myeloid leukemia cells and, combined with imatinib, produces a synergistic downregulation of ERK/SPK1 signaling. Imatinib Mesylate 108-116 sphingosine kinase 1 Homo sapiens 163-167 21857502-6 2012 Extracellular signal-regulated kinase/sphingosine kinase 1 signaling downstream of lipid raft-activated signaling pathways was significantly inhibited by treatment of cells with a combination of MbetaCD and imatinib compared with treatment with either agent alone. methyl-beta-cyclodextrin 195-202 sphingosine kinase 1 Homo sapiens 38-58 21857502-6 2012 Extracellular signal-regulated kinase/sphingosine kinase 1 signaling downstream of lipid raft-activated signaling pathways was significantly inhibited by treatment of cells with a combination of MbetaCD and imatinib compared with treatment with either agent alone. Imatinib Mesylate 207-215 sphingosine kinase 1 Homo sapiens 38-58 22312298-6 2012 We also determined whether isoflurane modulates the expression and activity of sphingosine kinase-1 (SK1) and induces the phosphorylation of extracellular signal regulated kinase (ERK MAPK) as both enzymes are known to protect against cell death. Isoflurane 27-37 sphingosine kinase 1 Homo sapiens 79-104 22312298-8 2012 Mechanistically, isoflurane induces the phosphorylation of ERK MAPK and increased SK1 expression and activity in EA.hy926 cells. Isoflurane 17-27 sphingosine kinase 1 Homo sapiens 82-85 22312298-9 2012 Finally, selective blockade of SK1 (with SKI-II) or S1P(1) receptor (with W146) abolished the anti-apoptotic effects of isoflurane. Isoflurane 120-130 sphingosine kinase 1 Homo sapiens 31-34 22312298-10 2012 Taken together, we demonstrate that isoflurane, in addition to its potent analgesic and anesthetic properties, protects against endothelial apoptosis most likely via SK1 and ERK MAPK activation. Isoflurane 36-46 sphingosine kinase 1 Homo sapiens 166-169 21932365-6 2012 Notably, TNFalpha provoked an NOX-dependent oxidative damage to sphingosine kinase-1, which generates sphingosine-1-phosphate, a ceramide metabolite associated with neurite outgrowth. sphingosine 1-phosphate 102-125 sphingosine kinase 1 Homo sapiens 64-84 21932365-6 2012 Notably, TNFalpha provoked an NOX-dependent oxidative damage to sphingosine kinase-1, which generates sphingosine-1-phosphate, a ceramide metabolite associated with neurite outgrowth. Ceramides 129-137 sphingosine kinase 1 Homo sapiens 64-84 21932365-8 2012 Blunting ceramide and ROS formation both rescued sphingosine kinase-1 activity and neurite outgrowth. Ceramides 9-17 sphingosine kinase 1 Homo sapiens 49-69 21932365-8 2012 Blunting ceramide and ROS formation both rescued sphingosine kinase-1 activity and neurite outgrowth. Reactive Oxygen Species 22-25 sphingosine kinase 1 Homo sapiens 49-69 22970244-7 2012 The SK2-selective inhibitor ABC294640 demonstrated the most pronounced effects on SK1 and SK2 mRNA expression, decrease of S1P levels, elevation of ceramide levels, cell cycle arrest, and inhibition of proliferation, migration and invasion. 3-(4-chlorophenyl)-adamantane-1-carboxylic acid (pyridin-4-ylmethyl)amide 28-37 sphingosine kinase 1 Homo sapiens 82-85 22761740-3 2012 The effects of SK-1 targeting were investigated by measuring the level of intracellular sphingosine, the activity of protein kinase C (PKC) and cell cycle regulators, and the mitotic index. Sphingosine 88-99 sphingosine kinase 1 Homo sapiens 15-19 22761740-5 2012 Downregulation of SK-1 or its pharmacologic inhibition increased intracellular sphingosine and decreased PKC activity as shown by reduced phosphorylation of PKC substrates. Sphingosine 79-90 sphingosine kinase 1 Homo sapiens 18-22 22761740-7 2012 SK-1 downregulation in MDA-MB-231 cells increased the number of cells with 4N and 8N DNA content, and similar effects were observed upon treatment with sphingosine or inhibitors of SK-1 or PKC. Sphingosine 152-163 sphingosine kinase 1 Homo sapiens 0-4 21906625-1 2011 The pleiotropic sphingolipid mediator, sphingosine-1-phosphate, produced in cells by two sphingosine kinase isoenzymes, SphK1 and SphK2, regulates many cellular and physiological processes important for homeostasis and development and pathophysiology. Sphingolipids 16-28 sphingosine kinase 1 Homo sapiens 120-125 21906625-1 2011 The pleiotropic sphingolipid mediator, sphingosine-1-phosphate, produced in cells by two sphingosine kinase isoenzymes, SphK1 and SphK2, regulates many cellular and physiological processes important for homeostasis and development and pathophysiology. sphingosine 1-phosphate 39-62 sphingosine kinase 1 Homo sapiens 120-125 21455605-3 2011 Sphingosine-1-phosphate (S1P) and glucosyleceramide, converted from ceramides by sphingosine kinase-1 (SK-1) and glucosyleceramide synthase (GCS) enzymes, respectively, inhibit apoptosis and develop resistance to chemotherapeutic drugs. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 81-107 21455605-3 2011 Sphingosine-1-phosphate (S1P) and glucosyleceramide, converted from ceramides by sphingosine kinase-1 (SK-1) and glucosyleceramide synthase (GCS) enzymes, respectively, inhibit apoptosis and develop resistance to chemotherapeutic drugs. glucosyleceramide 34-51 sphingosine kinase 1 Homo sapiens 81-107 21455605-3 2011 Sphingosine-1-phosphate (S1P) and glucosyleceramide, converted from ceramides by sphingosine kinase-1 (SK-1) and glucosyleceramide synthase (GCS) enzymes, respectively, inhibit apoptosis and develop resistance to chemotherapeutic drugs. Ceramides 68-77 sphingosine kinase 1 Homo sapiens 81-107 21455605-10 2011 It was shown for the first time that dasatinib induces apoptosis through downregulating expression levels of antiapoptotic SK-1 but not GCS, and upregulating expression levels of ceramide synthase (CerS) genes, especially CerS1, in K562 cells. Dasatinib 37-46 sphingosine kinase 1 Homo sapiens 123-127 21455605-11 2011 On the other hand, dasatinib downregulates expression levels of both GCS and SK-1 and upregulate apoptotic CerS2, -5 and -6 genes in Meg-01 cells. Dasatinib 19-28 sphingosine kinase 1 Homo sapiens 77-81 22528436-1 2012 Sphingosine kinases (SK) 1 and 2 are unique lipid kinases that phosphorylate sphingosine to form -sphingosine-1-phosphate (S1P). Sphingosine 77-88 sphingosine kinase 1 Homo sapiens 0-32 22528436-1 2012 Sphingosine kinases (SK) 1 and 2 are unique lipid kinases that phosphorylate sphingosine to form -sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 98-121 sphingosine kinase 1 Homo sapiens 0-32 22528439-1 2012 Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its production, sphingosine kinase-1 (SphK-1), are thought to be dysregulated in multiple human diseases including cancer, multiple sclerosis (MS), diabetes, neurological diseases, fibrosis, and certain pathologies associated with impaired angiogenesis such as, age-related macular degeneration (AMD). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 87-107 22528439-1 2012 Sphingosine-1-phosphate (S1P) and the enzyme primarily responsible for its production, sphingosine kinase-1 (SphK-1), are thought to be dysregulated in multiple human diseases including cancer, multiple sclerosis (MS), diabetes, neurological diseases, fibrosis, and certain pathologies associated with impaired angiogenesis such as, age-related macular degeneration (AMD). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 109-115 22528446-2 2012 There are emerging evidence that many cell types can produce and release S1P; therefore, the quantification of its intracellular and extracellular content as well as the activity of sphingosine kinase (SphK), the enzyme responsible of S1P synthesis, is crucial to attribute to the SphK/S1P axis a functional significance in response to many different stimuli and in physiopathological conditions.This chapter describes experimental procedures to measure intracellular S1P formation in skeletal muscle cells and skeletal muscle fibers by using sphingolipid precursors. Sphingolipids 543-555 sphingosine kinase 1 Homo sapiens 182-200 22528446-2 2012 There are emerging evidence that many cell types can produce and release S1P; therefore, the quantification of its intracellular and extracellular content as well as the activity of sphingosine kinase (SphK), the enzyme responsible of S1P synthesis, is crucial to attribute to the SphK/S1P axis a functional significance in response to many different stimuli and in physiopathological conditions.This chapter describes experimental procedures to measure intracellular S1P formation in skeletal muscle cells and skeletal muscle fibers by using sphingolipid precursors. Sphingolipids 543-555 sphingosine kinase 1 Homo sapiens 202-206 22970244-5 2012 Therefore, we characterized several SK-inhibitory compounds which revealed differential inhibitory effects on SK1 and SK2 as follows: SKI-II and ABC294735 are SK1/2-dual inhibitors; CB5468139 is a SK1-selective inhibitor; and ABC294640 is a SK2-selective inhibitor. N-(3-chloro-1,4-dioxonaphthalen-2-yl)-N-cyclohexylacetamide 182-191 sphingosine kinase 1 Homo sapiens 110-113 20473784-1 2011 The pro-apoptotic lipid sphingosine is phosphorylated by sphingosine kinases 1 and 2 (SK1 and SK2) to generate the mitogenic lipid sphingosine-1-phosphate (S1P). Sphingosine 24-35 sphingosine kinase 1 Homo sapiens 86-89 20473784-1 2011 The pro-apoptotic lipid sphingosine is phosphorylated by sphingosine kinases 1 and 2 (SK1 and SK2) to generate the mitogenic lipid sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 131-154 sphingosine kinase 1 Homo sapiens 86-89 20473784-1 2011 The pro-apoptotic lipid sphingosine is phosphorylated by sphingosine kinases 1 and 2 (SK1 and SK2) to generate the mitogenic lipid sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 156-159 sphingosine kinase 1 Homo sapiens 86-89 22016563-3 2011 Pretreating cells with protein kinase C (PKC) inhibitor, bisindolylmaleimide-I, abolished IGF-II-stimulated translocation of green fluorescent protein (GFP)-tagged SK1 to the plasma membrane and activation of endogenous SK1, implicating PKC as an upstream regulator of SK1. bisindolylmaleimide I 57-78 sphingosine kinase 1 Homo sapiens 164-167 22016563-3 2011 Pretreating cells with protein kinase C (PKC) inhibitor, bisindolylmaleimide-I, abolished IGF-II-stimulated translocation of green fluorescent protein (GFP)-tagged SK1 to the plasma membrane and activation of endogenous SK1, implicating PKC as an upstream regulator of SK1. bisindolylmaleimide I 57-78 sphingosine kinase 1 Homo sapiens 220-223 22016563-3 2011 Pretreating cells with protein kinase C (PKC) inhibitor, bisindolylmaleimide-I, abolished IGF-II-stimulated translocation of green fluorescent protein (GFP)-tagged SK1 to the plasma membrane and activation of endogenous SK1, implicating PKC as an upstream regulator of SK1. bisindolylmaleimide I 57-78 sphingosine kinase 1 Homo sapiens 220-223 22016563-9 2011 Pretreating cells with the PLC inhibitor, U73122, attenuated IGF-II-dependent DAG production and PKCbeta2 phosphorylation, blocked membrane translocation of the kinase-deficient GFP-PKCbeta2 (K371R) mutant, and reduced sphingosine 1-phosphate production, suggesting that PLC/PKCbeta2 are upstream regulators of SK1 in the pathway. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 42-48 sphingosine kinase 1 Homo sapiens 311-314 21769916-8 2011 SPHK1-WT cells showed enhanced GDNF-induced sphingosine 1-phosphate (S1P) secretion compared with mock- and SPHK1-DN cells. sphingosine 1-phosphate 44-67 sphingosine kinase 1 Homo sapiens 0-5 21199328-8 2011 Notably, EC was associated with the activation of sphingosine kinase 1 (SphK1) and with increased endogenous S1P synthesis, further stressing the relevance of S1P in skeletal muscle protection and repair/regeneration. ec 9-11 sphingosine kinase 1 Homo sapiens 50-70 21199328-8 2011 Notably, EC was associated with the activation of sphingosine kinase 1 (SphK1) and with increased endogenous S1P synthesis, further stressing the relevance of S1P in skeletal muscle protection and repair/regeneration. ec 9-11 sphingosine kinase 1 Homo sapiens 72-77 21769916-8 2011 SPHK1-WT cells showed enhanced GDNF-induced sphingosine 1-phosphate (S1P) secretion compared with mock- and SPHK1-DN cells. sphingosine 1-phosphate 69-72 sphingosine kinase 1 Homo sapiens 0-5 21896638-1 2011 Sphingosine kinases (SK) regulate the balance between proapoptotic ceramides and mitogenic sphingosine-1-phosphate (S1P); however, the functions of the two isoenzymes (SK1 and SK2) in tumor cells are not well defined. sphingosine 1-phosphate 91-114 sphingosine kinase 1 Homo sapiens 168-171 21896638-2 2011 Therefore, RNA interference was used to assess the individual roles of SK1 and SK2 in tumor cell sphingolipid metabolism, proliferation, and migration/invasion. Sphingolipids 97-109 sphingosine kinase 1 Homo sapiens 71-74 21896638-4 2011 Ablation of SK1 did not affect mRNA or protein levels of SK2 and reduced intracellular levels of S1P while elevating ceramide levels. Ceramides 117-125 sphingosine kinase 1 Homo sapiens 12-15 22331716-3 2011 In this study, we aimed to determine the roles of sphingosine kinase-1 (SK-1) and glucosylceramide synthase (GCS) genes in paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel induced apoptosis in human MCF-7 breast cancer cells. Paclitaxel 123-133 sphingosine kinase 1 Homo sapiens 50-76 22331716-3 2011 In this study, we aimed to determine the roles of sphingosine kinase-1 (SK-1) and glucosylceramide synthase (GCS) genes in paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel induced apoptosis in human MCF-7 breast cancer cells. Doxorubicin 135-146 sphingosine kinase 1 Homo sapiens 50-76 22331716-3 2011 In this study, we aimed to determine the roles of sphingosine kinase-1 (SK-1) and glucosylceramide synthase (GCS) genes in paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel induced apoptosis in human MCF-7 breast cancer cells. Tamoxifen 148-157 sphingosine kinase 1 Homo sapiens 50-76 22331716-3 2011 In this study, we aimed to determine the roles of sphingosine kinase-1 (SK-1) and glucosylceramide synthase (GCS) genes in paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel induced apoptosis in human MCF-7 breast cancer cells. Docetaxel 180-189 sphingosine kinase 1 Homo sapiens 50-76 22331716-8 2011 Paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel application downregulated SK-1 expression while paclitaxel, tamoxifen, cyclophosphamide and docetaxel but not doxorubicin downregulated GCS comparing to untreated control cells. Paclitaxel 0-10 sphingosine kinase 1 Homo sapiens 93-97 22331716-8 2011 Paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel application downregulated SK-1 expression while paclitaxel, tamoxifen, cyclophosphamide and docetaxel but not doxorubicin downregulated GCS comparing to untreated control cells. Doxorubicin 12-23 sphingosine kinase 1 Homo sapiens 93-97 22331716-8 2011 Paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel application downregulated SK-1 expression while paclitaxel, tamoxifen, cyclophosphamide and docetaxel but not doxorubicin downregulated GCS comparing to untreated control cells. Tamoxifen 25-34 sphingosine kinase 1 Homo sapiens 93-97 22331716-8 2011 Paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel application downregulated SK-1 expression while paclitaxel, tamoxifen, cyclophosphamide and docetaxel but not doxorubicin downregulated GCS comparing to untreated control cells. Cyclophosphamide 36-52 sphingosine kinase 1 Homo sapiens 93-97 22331716-8 2011 Paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel application downregulated SK-1 expression while paclitaxel, tamoxifen, cyclophosphamide and docetaxel but not doxorubicin downregulated GCS comparing to untreated control cells. Docetaxel 57-66 sphingosine kinase 1 Homo sapiens 93-97 22331716-8 2011 Paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel application downregulated SK-1 expression while paclitaxel, tamoxifen, cyclophosphamide and docetaxel but not doxorubicin downregulated GCS comparing to untreated control cells. Docetaxel 159-168 sphingosine kinase 1 Homo sapiens 93-97 22331716-8 2011 Paclitaxel, doxorubicin, tamoxifen, cyclophosphamide and docetaxel application downregulated SK-1 expression while paclitaxel, tamoxifen, cyclophosphamide and docetaxel but not doxorubicin downregulated GCS comparing to untreated control cells. Doxorubicin 177-188 sphingosine kinase 1 Homo sapiens 93-97 22331716-9 2011 CONCLUSION: These results show for the first time that these agents induce apoptosis in MCF-7 cells by downregulating the antiapoptotic SK-1 and GCS genes that may result in accumulation of apoptotic ceramides. Ceramides 200-209 sphingosine kinase 1 Homo sapiens 136-140 21912422-1 2011 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipid sphingosine into the antiapoptotic lipid sphingosine-1-phosphate, which activates the signal transduction pathways that lead to cell proliferation, migration, activation of the inflammatory response and impairment of apoptosis. Sphingosine 108-119 sphingosine kinase 1 Homo sapiens 0-20 21912422-1 2011 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipid sphingosine into the antiapoptotic lipid sphingosine-1-phosphate, which activates the signal transduction pathways that lead to cell proliferation, migration, activation of the inflammatory response and impairment of apoptosis. Sphingosine 108-119 sphingosine kinase 1 Homo sapiens 22-25 21912422-1 2011 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipid sphingosine into the antiapoptotic lipid sphingosine-1-phosphate, which activates the signal transduction pathways that lead to cell proliferation, migration, activation of the inflammatory response and impairment of apoptosis. sphingosine 1-phosphate 149-172 sphingosine kinase 1 Homo sapiens 0-20 21912422-1 2011 Sphingosine kinase 1 (SK1) is a lipid enzyme with oncogenic properties that converts the proapoptotic lipid sphingosine into the antiapoptotic lipid sphingosine-1-phosphate, which activates the signal transduction pathways that lead to cell proliferation, migration, activation of the inflammatory response and impairment of apoptosis. sphingosine 1-phosphate 149-172 sphingosine kinase 1 Homo sapiens 22-25 21912422-6 2011 With the recent discovery that clinically approved drug fingolimod has SK1-inhibiting properties, SK1 has gained significant attention from both clinicians and the pharmaceutical industry and it is hoped that trials of newly developed SK1 inhibitors might follow soon. Fingolimod Hydrochloride 56-66 sphingosine kinase 1 Homo sapiens 71-74 21912422-6 2011 With the recent discovery that clinically approved drug fingolimod has SK1-inhibiting properties, SK1 has gained significant attention from both clinicians and the pharmaceutical industry and it is hoped that trials of newly developed SK1 inhibitors might follow soon. Fingolimod Hydrochloride 56-66 sphingosine kinase 1 Homo sapiens 98-101 21912422-6 2011 With the recent discovery that clinically approved drug fingolimod has SK1-inhibiting properties, SK1 has gained significant attention from both clinicians and the pharmaceutical industry and it is hoped that trials of newly developed SK1 inhibitors might follow soon. Fingolimod Hydrochloride 56-66 sphingosine kinase 1 Homo sapiens 98-101 21392092-0 2011 Sphingosine kinase 1 pathway is involved in melatonin-induced HIF-1alpha inactivation in hypoxic PC-3 prostate cancer cells. Melatonin 44-53 sphingosine kinase 1 Homo sapiens 0-20 21392092-6 2011 Consistently, siRNA-SPHK1 and sphingosine kinase inhibitor (SKI) effectively blocked the expression of HIF-1alpha, phospho-AKT and vascular endothelial growth factor (VEGF) production in PC-3 cells under hypoxia, suggesting the role of SPHK1 in melatonin-inhibited HIF-1alpha accumulation. Melatonin 245-254 sphingosine kinase 1 Homo sapiens 20-25 21392092-2 2011 Thus, in the present study, the biological mechanisms of melatonin were elucidated in association with SPHK1 pathway in PC-3 prostate cancer cells under hypoxia. Melatonin 57-66 sphingosine kinase 1 Homo sapiens 103-108 21392092-7 2011 Moreover, reactive oxygen species (ROS) scavenger N-acteylcysteine enhanced melatonin-inhibited HIF-1alpha expression and SPHK1 activity. Reactive Oxygen Species 10-33 sphingosine kinase 1 Homo sapiens 122-127 21392092-4 2011 Also, melatonin decreased SPHK1 activity in PC-3 cells during hypoxia. Melatonin 6-15 sphingosine kinase 1 Homo sapiens 26-31 21392092-7 2011 Moreover, reactive oxygen species (ROS) scavenger N-acteylcysteine enhanced melatonin-inhibited HIF-1alpha expression and SPHK1 activity. Reactive Oxygen Species 35-38 sphingosine kinase 1 Homo sapiens 122-127 21756066-4 2011 For the first time, The results of this study showed for the first time that nilotinib induces apoptosis through upregulating ceramide synthase genes and downregulating SK-1 in CML cells in addition to inhibition of BCR/ABL. nilotinib 77-86 sphingosine kinase 1 Homo sapiens 169-173 21392092-7 2011 Moreover, reactive oxygen species (ROS) scavenger N-acteylcysteine enhanced melatonin-inhibited HIF-1alpha expression and SPHK1 activity. n-acteylcysteine 50-66 sphingosine kinase 1 Homo sapiens 122-127 21392092-7 2011 Moreover, reactive oxygen species (ROS) scavenger N-acteylcysteine enhanced melatonin-inhibited HIF-1alpha expression and SPHK1 activity. Melatonin 76-85 sphingosine kinase 1 Homo sapiens 122-127 21392092-8 2011 Overall, our findings suggest that melatonin suppresses HIF-1alpha accumulation via inhibition of SPHK1 pathway and ROS generation in PC-3 cells under hypoxia. Melatonin 35-44 sphingosine kinase 1 Homo sapiens 98-103 21392556-10 2011 CONCLUSIONS: These results demonstrate that SphK1 is upregulated in imatinib-resistant K562 cells by a pathway contingent on a phosphoinositide 3-kinase/AKT2/mammalian target of rapamycin signaling pathway. Imatinib Mesylate 68-76 sphingosine kinase 1 Homo sapiens 44-49 21392556-0 2011 Sphingosine kinase 1 overexpression is regulated by signaling through PI3K, AKT2, and mTOR in imatinib-resistant chronic myeloid leukemia cells. Imatinib Mesylate 94-102 sphingosine kinase 1 Homo sapiens 0-20 21392556-7 2011 Furthermore, expression levels of sphingosine kinase 1 (SphK1) were increased significantly in resistant cells, channeling sphingoid bases to the SphK1 pathway and activating sphingosine-1-phosphate-dependent tyrosine phosphorylation pathways that include the adaptor protein Crk. sphingoid bases 123-138 sphingosine kinase 1 Homo sapiens 34-54 21392556-7 2011 Furthermore, expression levels of sphingosine kinase 1 (SphK1) were increased significantly in resistant cells, channeling sphingoid bases to the SphK1 pathway and activating sphingosine-1-phosphate-dependent tyrosine phosphorylation pathways that include the adaptor protein Crk. sphingoid bases 123-138 sphingosine kinase 1 Homo sapiens 56-61 21392556-7 2011 Furthermore, expression levels of sphingosine kinase 1 (SphK1) were increased significantly in resistant cells, channeling sphingoid bases to the SphK1 pathway and activating sphingosine-1-phosphate-dependent tyrosine phosphorylation pathways that include the adaptor protein Crk. sphingoid bases 123-138 sphingosine kinase 1 Homo sapiens 146-151 21392556-7 2011 Furthermore, expression levels of sphingosine kinase 1 (SphK1) were increased significantly in resistant cells, channeling sphingoid bases to the SphK1 pathway and activating sphingosine-1-phosphate-dependent tyrosine phosphorylation pathways that include the adaptor protein Crk. sphingosine 1-phosphate 175-198 sphingosine kinase 1 Homo sapiens 34-54 21392556-7 2011 Furthermore, expression levels of sphingosine kinase 1 (SphK1) were increased significantly in resistant cells, channeling sphingoid bases to the SphK1 pathway and activating sphingosine-1-phosphate-dependent tyrosine phosphorylation pathways that include the adaptor protein Crk. sphingosine 1-phosphate 175-198 sphingosine kinase 1 Homo sapiens 56-61 21392556-7 2011 Furthermore, expression levels of sphingosine kinase 1 (SphK1) were increased significantly in resistant cells, channeling sphingoid bases to the SphK1 pathway and activating sphingosine-1-phosphate-dependent tyrosine phosphorylation pathways that include the adaptor protein Crk. Tyrosine 209-217 sphingosine kinase 1 Homo sapiens 34-54 21392556-7 2011 Furthermore, expression levels of sphingosine kinase 1 (SphK1) were increased significantly in resistant cells, channeling sphingoid bases to the SphK1 pathway and activating sphingosine-1-phosphate-dependent tyrosine phosphorylation pathways that include the adaptor protein Crk. Tyrosine 209-217 sphingosine kinase 1 Homo sapiens 56-61 21392556-8 2011 The partial inhibition of SphK1 activity by N,N-dimethylsphingosine or expression by small interfering RNA increased sensitivity to imatinib-induced apoptosis in resistant cells and returned BCR-ABL to baseline levels. N,N-dimethylsphingosine 44-67 sphingosine kinase 1 Homo sapiens 26-31 21392556-8 2011 The partial inhibition of SphK1 activity by N,N-dimethylsphingosine or expression by small interfering RNA increased sensitivity to imatinib-induced apoptosis in resistant cells and returned BCR-ABL to baseline levels. Imatinib Mesylate 132-140 sphingosine kinase 1 Homo sapiens 26-31 21546914-1 2011 The potent lipid mediator sphingosine-1-phosphate (S1P) is produced inside cells by two closely related kinases, sphingosine kinase 1 (SPHK1) and SPHK2, and has emerged as a crucial regulator of immunity. sphingosine 1-phosphate 26-49 sphingosine kinase 1 Homo sapiens 113-133 21546914-1 2011 The potent lipid mediator sphingosine-1-phosphate (S1P) is produced inside cells by two closely related kinases, sphingosine kinase 1 (SPHK1) and SPHK2, and has emerged as a crucial regulator of immunity. sphingosine 1-phosphate 26-49 sphingosine kinase 1 Homo sapiens 135-140 21546914-1 2011 The potent lipid mediator sphingosine-1-phosphate (S1P) is produced inside cells by two closely related kinases, sphingosine kinase 1 (SPHK1) and SPHK2, and has emerged as a crucial regulator of immunity. sphingosine 1-phosphate 51-54 sphingosine kinase 1 Homo sapiens 113-133 21546914-1 2011 The potent lipid mediator sphingosine-1-phosphate (S1P) is produced inside cells by two closely related kinases, sphingosine kinase 1 (SPHK1) and SPHK2, and has emerged as a crucial regulator of immunity. sphingosine 1-phosphate 51-54 sphingosine kinase 1 Homo sapiens 135-140 21495716-0 2011 Development of amidine-based sphingosine kinase 1 nanomolar inhibitors and reduction of sphingosine 1-phosphate in human leukemia cells. Amidines 15-22 sphingosine kinase 1 Homo sapiens 29-49 21495716-0 2011 Development of amidine-based sphingosine kinase 1 nanomolar inhibitors and reduction of sphingosine 1-phosphate in human leukemia cells. sphingosine 1-phosphate 88-111 sphingosine kinase 1 Homo sapiens 29-49 21495716-4 2011 Herein, we present the design and synthesis of amidine-based nanomolar SphK1 subtype-selective inhibitors. Amidines 47-54 sphingosine kinase 1 Homo sapiens 71-76 21495716-5 2011 A homology model of SphK1, trained with this library of amidine inhibitors, was then used to predict the activity of additional, more potent, inhibitors. Amidines 56-63 sphingosine kinase 1 Homo sapiens 20-25 21613209-3 2011 We sought to identify the kinase(s) involved in phosphorylation of CS-0777, anticipating sphingosine kinase (SPHK) 1 or 2 as likely candidates. Cesium 67-69 sphingosine kinase 1 Homo sapiens 89-116 21613209-5 2011 N,N-Dimethylsphingosine, an inhibitor of SPHK1 and -2, did not inhibit CS-0777 kinase activity. N,N-dimethylsphingosine 0-23 sphingosine kinase 1 Homo sapiens 41-53 21527515-1 2011 The mechanisms by which sphingosine kinase-1 (SK-1)/sphingosine 1-phosphate (S1P) activation contributes to imatinib resistance in chronic myeloid leukemia (CML) are unknown. Imatinib Mesylate 108-116 sphingosine kinase 1 Homo sapiens 24-44 21527515-1 2011 The mechanisms by which sphingosine kinase-1 (SK-1)/sphingosine 1-phosphate (S1P) activation contributes to imatinib resistance in chronic myeloid leukemia (CML) are unknown. Imatinib Mesylate 108-116 sphingosine kinase 1 Homo sapiens 46-50 21527515-2 2011 We show herein that increased SK-1/S1P enhances Bcr-Abl1 protein stability, through inhibition of its proteasomal degradation in imatinib-resistant K562/IMA-3 and LAMA-4/IMA human CML cells. Imatinib Mesylate 129-137 sphingosine kinase 1 Homo sapiens 30-38 21478254-8 2011 Downregulation of sphingosine kinase 1 (SphK1), but not SphK2, with siRNA or inhibition of SphK activity with an inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl) thiazole (CII) attenuated exogenously administrated S1P-induced EC permeability. N-[(1S)-2-methyl-1-(pyridin-4-ylcarbamoyl)propyl]cyclohexanecarboxamide 173-176 sphingosine kinase 1 Homo sapiens 18-38 21478254-8 2011 Downregulation of sphingosine kinase 1 (SphK1), but not SphK2, with siRNA or inhibition of SphK activity with an inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl) thiazole (CII) attenuated exogenously administrated S1P-induced EC permeability. N-[(1S)-2-methyl-1-(pyridin-4-ylcarbamoyl)propyl]cyclohexanecarboxamide 173-176 sphingosine kinase 1 Homo sapiens 40-45 21478254-8 2011 Downregulation of sphingosine kinase 1 (SphK1), but not SphK2, with siRNA or inhibition of SphK activity with an inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl) thiazole (CII) attenuated exogenously administrated S1P-induced EC permeability. sphingosine 1-phosphate 215-218 sphingosine kinase 1 Homo sapiens 18-38 21478254-8 2011 Downregulation of sphingosine kinase 1 (SphK1), but not SphK2, with siRNA or inhibition of SphK activity with an inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl) thiazole (CII) attenuated exogenously administrated S1P-induced EC permeability. sphingosine 1-phosphate 215-218 sphingosine kinase 1 Homo sapiens 40-45 21464128-1 2011 Sphingosine kinase 1 (SK1) catalyzes the conversion of sphingosine to the bioactive lipid sphingosine 1-phosphate. Sphingosine 55-66 sphingosine kinase 1 Homo sapiens 0-20 21464128-1 2011 Sphingosine kinase 1 (SK1) catalyzes the conversion of sphingosine to the bioactive lipid sphingosine 1-phosphate. Sphingosine 55-66 sphingosine kinase 1 Homo sapiens 22-25 21464128-1 2011 Sphingosine kinase 1 (SK1) catalyzes the conversion of sphingosine to the bioactive lipid sphingosine 1-phosphate. sphingosine 1-phosphate 90-113 sphingosine kinase 1 Homo sapiens 0-20 21464128-1 2011 Sphingosine kinase 1 (SK1) catalyzes the conversion of sphingosine to the bioactive lipid sphingosine 1-phosphate. sphingosine 1-phosphate 90-113 sphingosine kinase 1 Homo sapiens 22-25 21464128-2 2011 We have previously demonstrated that FTY720 and (S)-FTY720 vinylphosphonate are novel inhibitors of SK1 activity. Fingolimod Hydrochloride 37-43 sphingosine kinase 1 Homo sapiens 100-103 21464128-2 2011 We have previously demonstrated that FTY720 and (S)-FTY720 vinylphosphonate are novel inhibitors of SK1 activity. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 48-75 sphingosine kinase 1 Homo sapiens 100-103 21464128-3 2011 Here, we show that (S)-FTY720 vinylphosphonate binds to a putative allosteric site in SK1 contingent on formation of the enzyme-sphingosine complex. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 19-46 sphingosine kinase 1 Homo sapiens 86-89 21464128-3 2011 Here, we show that (S)-FTY720 vinylphosphonate binds to a putative allosteric site in SK1 contingent on formation of the enzyme-sphingosine complex. Sphingosine 128-139 sphingosine kinase 1 Homo sapiens 86-89 21464128-5 2011 A model is proposed in which (S)-FTY720 vinylphosphonate binding to and stabilization of the allosteric site might enhance the autoinhibitory effect on SK1 activity. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 29-56 sphingosine kinase 1 Homo sapiens 152-155 21464128-6 2011 Further evidence for the existence of allosteric site(s) in SK1 was demonstrated by data showing that two new FTY720 analogues (a conjugate of sphingosine with a fluorophore and (S)-FTY720 regioisomer) increased SK1 activity, suggesting relief of autoinhibition of SK1 activity. Sphingosine 143-154 sphingosine kinase 1 Homo sapiens 60-63 21464128-7 2011 Comparisons with the SK1 inhibitor, SKi or siRNA knockdown of SK1 indicated that (S)-FTY720 vinylphosphonate and FTY720 behave as typical SK1 inhibitors in preventing sphingosine 1-phosphate-stimulated rearrangement of actin in MCF-7 cells. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 81-108 sphingosine kinase 1 Homo sapiens 21-24 21464128-7 2011 Comparisons with the SK1 inhibitor, SKi or siRNA knockdown of SK1 indicated that (S)-FTY720 vinylphosphonate and FTY720 behave as typical SK1 inhibitors in preventing sphingosine 1-phosphate-stimulated rearrangement of actin in MCF-7 cells. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 81-108 sphingosine kinase 1 Homo sapiens 62-65 21464128-7 2011 Comparisons with the SK1 inhibitor, SKi or siRNA knockdown of SK1 indicated that (S)-FTY720 vinylphosphonate and FTY720 behave as typical SK1 inhibitors in preventing sphingosine 1-phosphate-stimulated rearrangement of actin in MCF-7 cells. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 81-108 sphingosine kinase 1 Homo sapiens 62-65 21464128-7 2011 Comparisons with the SK1 inhibitor, SKi or siRNA knockdown of SK1 indicated that (S)-FTY720 vinylphosphonate and FTY720 behave as typical SK1 inhibitors in preventing sphingosine 1-phosphate-stimulated rearrangement of actin in MCF-7 cells. sphingosine 1-phosphate 167-190 sphingosine kinase 1 Homo sapiens 62-65 21464128-7 2011 Comparisons with the SK1 inhibitor, SKi or siRNA knockdown of SK1 indicated that (S)-FTY720 vinylphosphonate and FTY720 behave as typical SK1 inhibitors in preventing sphingosine 1-phosphate-stimulated rearrangement of actin in MCF-7 cells. sphingosine 1-phosphate 167-190 sphingosine kinase 1 Homo sapiens 62-65 21392556-11 2011 We propose that SphK1 plays an important role in development of acquired resistance to imatinib in chronic myeloid leukemia cell lines. Imatinib Mesylate 87-95 sphingosine kinase 1 Homo sapiens 16-21 21543615-1 2011 Sphingosine kinase (SphK) 1 and 2 phosphorylate sphingosine to generate sphingosine-1-phosphate (S1P), a pluripotent lipophilic mediator implicated in a variety of cellular events. Sphingosine 48-59 sphingosine kinase 1 Homo sapiens 0-33 21543615-1 2011 Sphingosine kinase (SphK) 1 and 2 phosphorylate sphingosine to generate sphingosine-1-phosphate (S1P), a pluripotent lipophilic mediator implicated in a variety of cellular events. sphingosine 1-phosphate 72-95 sphingosine kinase 1 Homo sapiens 0-33 21543615-1 2011 Sphingosine kinase (SphK) 1 and 2 phosphorylate sphingosine to generate sphingosine-1-phosphate (S1P), a pluripotent lipophilic mediator implicated in a variety of cellular events. sphingosine 1-phosphate 97-100 sphingosine kinase 1 Homo sapiens 0-33 21216217-2 2011 The conventional assay of SphK enzymatic activity uses [gamma-(32)P]ATP and sphingosine as substrates, with the radiolabeled S1P product recovered by organic extraction, displayed by thin layer chromatography, and quantified by liquid scintillation counting. [gamma-(32)p]atp 55-71 sphingosine kinase 1 Homo sapiens 26-30 21216217-2 2011 The conventional assay of SphK enzymatic activity uses [gamma-(32)P]ATP and sphingosine as substrates, with the radiolabeled S1P product recovered by organic extraction, displayed by thin layer chromatography, and quantified by liquid scintillation counting. Sphingosine 76-87 sphingosine kinase 1 Homo sapiens 26-30 20889557-0 2010 High expression of sphingosine 1-phosphate receptors, S1P1 and S1P3, sphingosine kinase 1, and extracellular signal-regulated kinase-1/2 is associated with development of tamoxifen resistance in estrogen receptor-positive breast cancer patients. Tamoxifen 171-180 sphingosine kinase 1 Homo sapiens 69-89 21233411-6 2011 Treatment of neutrophil-like dHL-60 cells by DHS, a SphK inhibitor, and SphK siRNA inhibited fMLF-induced NOX2 activity. dhl-60 29-35 sphingosine kinase 1 Homo sapiens 52-56 21233411-6 2011 Treatment of neutrophil-like dHL-60 cells by DHS, a SphK inhibitor, and SphK siRNA inhibited fMLF-induced NOX2 activity. dhl-60 29-35 sphingosine kinase 1 Homo sapiens 72-76 21233411-7 2011 Sequential activation of cells by thapsigargin and the phorbol ester PMA revealed that SphK-regulated NOX2 activity relies on intracellular Ca(2+) store depletion. Thapsigargin 34-46 sphingosine kinase 1 Homo sapiens 87-91 21233411-7 2011 Sequential activation of cells by thapsigargin and the phorbol ester PMA revealed that SphK-regulated NOX2 activity relies on intracellular Ca(2+) store depletion. Phorbol Esters 55-68 sphingosine kinase 1 Homo sapiens 87-91 21575515-2 2011 METHODS: Phorbol 12-myristate 13-acetate (PMA) was used to induce the activity of SphK1 and N, N-dimethylsphingosine (DMS) was used to suppress the activity of SphK1. Tetradecanoylphorbol Acetate 9-40 sphingosine kinase 1 Homo sapiens 82-87 21575515-2 2011 METHODS: Phorbol 12-myristate 13-acetate (PMA) was used to induce the activity of SphK1 and N, N-dimethylsphingosine (DMS) was used to suppress the activity of SphK1. Tetradecanoylphorbol Acetate 42-45 sphingosine kinase 1 Homo sapiens 82-87 21575515-2 2011 METHODS: Phorbol 12-myristate 13-acetate (PMA) was used to induce the activity of SphK1 and N, N-dimethylsphingosine (DMS) was used to suppress the activity of SphK1. N,N-dimethylsphingosine 92-116 sphingosine kinase 1 Homo sapiens 160-165 21575515-2 2011 METHODS: Phorbol 12-myristate 13-acetate (PMA) was used to induce the activity of SphK1 and N, N-dimethylsphingosine (DMS) was used to suppress the activity of SphK1. dms 118-121 sphingosine kinase 1 Homo sapiens 160-165 20838377-6 2011 Here, we show that the guanine nucleotide-bound state of eEF1A regulates its ability to activate SK1, with eEF1A.GDP, but not eEF1A.GTP, enhancing SK1 activity in vitro. Guanosine Diphosphate 113-116 sphingosine kinase 1 Homo sapiens 147-150 20838377-0 2011 Guanine nucleotides regulate sphingosine kinase 1 activation by eukaryotic elongation factor 1A and provide a mechanism for eEF1A-associated oncogenesis. Guanine Nucleotides 0-19 sphingosine kinase 1 Homo sapiens 29-49 20838377-1 2011 Sphingosine kinase 1 (SK1) catalyses the formation of bioactive phospholipid sphingosine 1-phosphate (S1P). phospholipid sphingosine 1-phosphate 64-100 sphingosine kinase 1 Homo sapiens 0-20 20838377-1 2011 Sphingosine kinase 1 (SK1) catalyses the formation of bioactive phospholipid sphingosine 1-phosphate (S1P). phospholipid sphingosine 1-phosphate 64-100 sphingosine kinase 1 Homo sapiens 22-25 20838377-6 2011 Here, we show that the guanine nucleotide-bound state of eEF1A regulates its ability to activate SK1, with eEF1A.GDP, but not eEF1A.GTP, enhancing SK1 activity in vitro. Guanine Nucleotides 23-41 sphingosine kinase 1 Homo sapiens 97-100 20838377-6 2011 Here, we show that the guanine nucleotide-bound state of eEF1A regulates its ability to activate SK1, with eEF1A.GDP, but not eEF1A.GTP, enhancing SK1 activity in vitro. Guanine Nucleotides 23-41 sphingosine kinase 1 Homo sapiens 147-150 20838377-6 2011 Here, we show that the guanine nucleotide-bound state of eEF1A regulates its ability to activate SK1, with eEF1A.GDP, but not eEF1A.GTP, enhancing SK1 activity in vitro. Guanosine Diphosphate 113-116 sphingosine kinase 1 Homo sapiens 97-100 20838377-7 2011 Furthermore, we show that enhancing cellular eEF1A.GDP levels through expression of a guanine nucleotide dissociation inhibitor of eEF1A, translationally controlled tumour protein (TCTP), increased SK1 activity in cells. Guanosine Diphosphate 51-54 sphingosine kinase 1 Homo sapiens 198-201 20838377-7 2011 Furthermore, we show that enhancing cellular eEF1A.GDP levels through expression of a guanine nucleotide dissociation inhibitor of eEF1A, translationally controlled tumour protein (TCTP), increased SK1 activity in cells. Guanine Nucleotides 86-104 sphingosine kinase 1 Homo sapiens 198-201 21035490-4 2011 However, whereas various components of the sphingolipid and p53 pathways may simultaneously function to elicit apoptosis and/or growth inhibition, SMase and SK1 may undergo explicit regulation by p53 that could contribute to ceramide-induced senescence in cells. Ceramides 225-233 sphingosine kinase 1 Homo sapiens 157-160 21075134-5 2011 In the work described here we explore how subcellular localization of sphingosine kinase-1 affects the downstream metabolism of sphingosine-1-phosphate and the access of sphingosine kinase to its substrates. sphingosine 1-phosphate 128-151 sphingosine kinase 1 Homo sapiens 70-90 20883472-0 2011 Loss of sphingosine kinase-1 in carcinoma cells increases formation of reactive oxygen species and sensitivity to doxorubicin-induced DNA damage. Reactive Oxygen Species 71-94 sphingosine kinase 1 Homo sapiens 8-28 20883472-0 2011 Loss of sphingosine kinase-1 in carcinoma cells increases formation of reactive oxygen species and sensitivity to doxorubicin-induced DNA damage. Doxorubicin 114-125 sphingosine kinase 1 Homo sapiens 8-28 20883472-9 2011 SK-1 knock-down (SK-1kd) cells revealed dysfunctional extracellular signal-regulated protein kinase and PKB/Akt cascades, and contained increased levels of ROS. Reactive Oxygen Species 156-159 sphingosine kinase 1 Homo sapiens 0-4 20883472-15 2011 Targeted inhibition of SK-1 represents a promising approach to sensitize cells to DNA damage and facilitate apoptosis upon doxorubicin treatment. Doxorubicin 123-134 sphingosine kinase 1 Homo sapiens 23-27 21500096-4 2011 Glucosylceramide synthase (GCS) and sphingosine kinase-1 (SK-1) enzymes can convert ceramides to antiapoptotic molecules, glucosylceramide, and sphingosine-1-phosphate, respectively. Ceramides 84-93 sphingosine kinase 1 Homo sapiens 36-62 21500096-4 2011 Glucosylceramide synthase (GCS) and sphingosine kinase-1 (SK-1) enzymes can convert ceramides to antiapoptotic molecules, glucosylceramide, and sphingosine-1-phosphate, respectively. Glucosylceramides 122-138 sphingosine kinase 1 Homo sapiens 36-62 21500096-4 2011 Glucosylceramide synthase (GCS) and sphingosine kinase-1 (SK-1) enzymes can convert ceramides to antiapoptotic molecules, glucosylceramide, and sphingosine-1-phosphate, respectively. sphingosine 1-phosphate 144-167 sphingosine kinase 1 Homo sapiens 36-62 21500096-5 2011 C8:ceramide, an important cell-permeable analogue of natural ceramides, increases intracellular ceramide levels significantly, while 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) and SK-1 inhibitor increase accumulation of ceramides by inhibiting GCS and SK-1, respectively. Ceramides 3-11 sphingosine kinase 1 Homo sapiens 193-197 21500096-5 2011 C8:ceramide, an important cell-permeable analogue of natural ceramides, increases intracellular ceramide levels significantly, while 1-phenyl-2-decanoylamino-3-morpholino-1-propanol (PDMP) and SK-1 inhibitor increase accumulation of ceramides by inhibiting GCS and SK-1, respectively. Ceramides 3-11 sphingosine kinase 1 Homo sapiens 265-269 21500096-8 2011 There were synergistic cytotoxic and apoptotic effects of resveratrol with coadministration of C8:ceramide, PDMP, and SK-1 inhibitor. Resveratrol 58-69 sphingosine kinase 1 Homo sapiens 118-122 21500096-9 2011 Interestingly, there were also significant increases in expression levels of LASS genes and decreases in expression levels of GCS and SK-1 in K562 cells in response to resveratrol. Resveratrol 168-179 sphingosine kinase 1 Homo sapiens 134-138 22812190-0 2011 Inhibition of SPHK1 suppresses phorbol 12-myristate 13-acetate-induced metastatic phenotype in colorectal cancer HT-29 cells. Tetradecanoylphorbol Acetate 31-62 sphingosine kinase 1 Homo sapiens 14-19 22812190-6 2011 PMA induced a metastatic phenotype in colorectal cancer cells, as indicated by cell viability, migration and invasion capacity, and ERK1/2 phosphorylation, whereas SPHK1 siRNA transfection suppressed the metastatic phenotype of tumor cells and antagonized PMA"s effects. Tetradecanoylphorbol Acetate 256-259 sphingosine kinase 1 Homo sapiens 164-169 22812190-8 2011 Suppression of SPHK1 expression suppresses the PMA-induced metastatic phenotype via ERK1/2 phosphorylation in human colorectal cancer cells. Tetradecanoylphorbol Acetate 47-50 sphingosine kinase 1 Homo sapiens 15-20 20926375-0 2010 The sphingosine kinase 1 inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole induces proteasomal degradation of sphingosine kinase 1 in mammalian cells. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 35-82 sphingosine kinase 1 Homo sapiens 4-24 20926375-0 2010 The sphingosine kinase 1 inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole induces proteasomal degradation of sphingosine kinase 1 in mammalian cells. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 35-82 sphingosine kinase 1 Homo sapiens 118-138 20926375-1 2010 Sphingosine kinase 1 (SK1) is an enzyme that catalyzes the phosphorylation of sphingosine to produce the bioactive lipid sphingosine 1-phosphate (S1P). Sphingosine 78-89 sphingosine kinase 1 Homo sapiens 0-20 20926375-1 2010 Sphingosine kinase 1 (SK1) is an enzyme that catalyzes the phosphorylation of sphingosine to produce the bioactive lipid sphingosine 1-phosphate (S1P). Sphingosine 78-89 sphingosine kinase 1 Homo sapiens 22-25 20926375-1 2010 Sphingosine kinase 1 (SK1) is an enzyme that catalyzes the phosphorylation of sphingosine to produce the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 121-144 sphingosine kinase 1 Homo sapiens 0-20 20926375-1 2010 Sphingosine kinase 1 (SK1) is an enzyme that catalyzes the phosphorylation of sphingosine to produce the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 121-144 sphingosine kinase 1 Homo sapiens 22-25 20926375-2 2010 We demonstrate here that the SK1 inhibitor, SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole) induces the proteasomal degradation of SK1 in human pulmonary artery smooth muscle cells, androgen-sensitive LNCaP prostate cancer cells, MCF-7 and MCF-7 HER2 breast cancer cells and that this is likely mediated by ceramide as a consequence of catalytic inhibition of SK1 by SKi. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 49-96 sphingosine kinase 1 Homo sapiens 29-32 20926375-2 2010 We demonstrate here that the SK1 inhibitor, SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole) induces the proteasomal degradation of SK1 in human pulmonary artery smooth muscle cells, androgen-sensitive LNCaP prostate cancer cells, MCF-7 and MCF-7 HER2 breast cancer cells and that this is likely mediated by ceramide as a consequence of catalytic inhibition of SK1 by SKi. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 49-96 sphingosine kinase 1 Homo sapiens 137-140 20926375-2 2010 We demonstrate here that the SK1 inhibitor, SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole) induces the proteasomal degradation of SK1 in human pulmonary artery smooth muscle cells, androgen-sensitive LNCaP prostate cancer cells, MCF-7 and MCF-7 HER2 breast cancer cells and that this is likely mediated by ceramide as a consequence of catalytic inhibition of SK1 by SKi. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 49-96 sphingosine kinase 1 Homo sapiens 137-140 20926375-2 2010 We demonstrate here that the SK1 inhibitor, SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole) induces the proteasomal degradation of SK1 in human pulmonary artery smooth muscle cells, androgen-sensitive LNCaP prostate cancer cells, MCF-7 and MCF-7 HER2 breast cancer cells and that this is likely mediated by ceramide as a consequence of catalytic inhibition of SK1 by SKi. Ceramides 313-321 sphingosine kinase 1 Homo sapiens 29-32 20926375-2 2010 We demonstrate here that the SK1 inhibitor, SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole) induces the proteasomal degradation of SK1 in human pulmonary artery smooth muscle cells, androgen-sensitive LNCaP prostate cancer cells, MCF-7 and MCF-7 HER2 breast cancer cells and that this is likely mediated by ceramide as a consequence of catalytic inhibition of SK1 by SKi. Ceramides 313-321 sphingosine kinase 1 Homo sapiens 137-140 20926375-2 2010 We demonstrate here that the SK1 inhibitor, SKi (2-(p-hydroxyanilino)-4-(p-chlorophenyl)thiazole) induces the proteasomal degradation of SK1 in human pulmonary artery smooth muscle cells, androgen-sensitive LNCaP prostate cancer cells, MCF-7 and MCF-7 HER2 breast cancer cells and that this is likely mediated by ceramide as a consequence of catalytic inhibition of SK1 by SKi. Ceramides 313-321 sphingosine kinase 1 Homo sapiens 137-140 21257722-3 2011 Safingol, (l-threo-dihydrosphingosine) is a putative inhibitor of SphK. safingol 0-8 sphingosine kinase 1 Homo sapiens 66-70 21257722-3 2011 Safingol, (l-threo-dihydrosphingosine) is a putative inhibitor of SphK. safingol 11-37 sphingosine kinase 1 Homo sapiens 66-70 21257722-19 2011 CONCLUSIONS: Safingol, the first putative SphK inhibitor to enter clinical trials, can be safely administered in combination with cisplatin. safingol 13-21 sphingosine kinase 1 Homo sapiens 42-46 21325072-2 2011 EXPERIMENTAL DESIGN: The correlation of SPHK1 expression and clinical features of NSCLC was analyzed in 218 paraffin-embedded archived NSCLC specimens by immunohistochemical analysis. Paraffin 108-116 sphingosine kinase 1 Homo sapiens 40-45 21325072-6 2011 Upregulation of SPHK1 significantly inhibited doxorubicin- or docetaxel-induced apoptosis, associated with induction of antiapoptotic proteins Bcl-xl, c-IAP1, c-IAP2, and TRAF1. Doxorubicin 46-57 sphingosine kinase 1 Homo sapiens 16-21 21325072-6 2011 Upregulation of SPHK1 significantly inhibited doxorubicin- or docetaxel-induced apoptosis, associated with induction of antiapoptotic proteins Bcl-xl, c-IAP1, c-IAP2, and TRAF1. Docetaxel 62-71 sphingosine kinase 1 Homo sapiens 16-21 21209394-4 2011 Therefore, in this study, we first analyzed the expression of SphK1 in human HNSCC tumor samples and normal head & neck tissues (n = 78 and 17, respectively) using immunohistochemistry. Adenosine Monophosphate 90-93 sphingosine kinase 1 Homo sapiens 62-67 20401667-10 2011 More importantly, gene expression analyses revealed that resveratrol-induced apoptosis via increasing expression levels of ceramide-generating genes and decreasing expression levels of antiapoptotic sphingosine kinase-1 and glucosylceramide synthase genes. Resveratrol 57-68 sphingosine kinase 1 Homo sapiens 199-219 21304987-3 2011 METHODOLOGY/PRINCIPAL FINDINGS: Inhibition of SphK activity with a SphK inhibitor 2-(p-Hydroxyanilino)-4-(p-Chlorophenyl) Thiazole or down-regulation of Sphk1, but not SphK2, with siRNA decreased S1P(int), and attenuated S1P(ext) or serum-induced motility of HPAECs. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 82-130 sphingosine kinase 1 Homo sapiens 46-50 21304987-3 2011 METHODOLOGY/PRINCIPAL FINDINGS: Inhibition of SphK activity with a SphK inhibitor 2-(p-Hydroxyanilino)-4-(p-Chlorophenyl) Thiazole or down-regulation of Sphk1, but not SphK2, with siRNA decreased S1P(int), and attenuated S1P(ext) or serum-induced motility of HPAECs. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 82-130 sphingosine kinase 1 Homo sapiens 67-71 21537471-1 2010 The sphingosine kinases, SK1 and SK2, produce the potent signaling lipid sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 73-96 sphingosine kinase 1 Homo sapiens 25-28 20649568-0 2010 Sphingosine kinase 1 is critically involved in nitric oxide-mediated human endothelial cell migration and tube formation. Nitric Oxide 47-59 sphingosine kinase 1 Homo sapiens 0-20 20930159-11 2010 Expression of a dominant-negative Sk1 mutant (Sk1(G82D)) eliminated both baseline spiral modiolar artery calcium sensitivity and TNF-alpha effects, whereas a nonphosphorylatable Sk1 mutant (Sk1(S225A)) blocked the effects of TNF-alpha only. Calcium 105-112 sphingosine kinase 1 Homo sapiens 34-37 20930159-11 2010 Expression of a dominant-negative Sk1 mutant (Sk1(G82D)) eliminated both baseline spiral modiolar artery calcium sensitivity and TNF-alpha effects, whereas a nonphosphorylatable Sk1 mutant (Sk1(S225A)) blocked the effects of TNF-alpha only. Calcium 105-112 sphingosine kinase 1 Homo sapiens 46-49 20930159-11 2010 Expression of a dominant-negative Sk1 mutant (Sk1(G82D)) eliminated both baseline spiral modiolar artery calcium sensitivity and TNF-alpha effects, whereas a nonphosphorylatable Sk1 mutant (Sk1(S225A)) blocked the effects of TNF-alpha only. Calcium 105-112 sphingosine kinase 1 Homo sapiens 46-49 20930159-11 2010 Expression of a dominant-negative Sk1 mutant (Sk1(G82D)) eliminated both baseline spiral modiolar artery calcium sensitivity and TNF-alpha effects, whereas a nonphosphorylatable Sk1 mutant (Sk1(S225A)) blocked the effects of TNF-alpha only. Calcium 105-112 sphingosine kinase 1 Homo sapiens 46-49 20352614-1 2010 Sphingosine kinase (SphK) is a key enzyme in modulating the levels of sphingosine 1-phosphate (S1P) as well as an important enzyme in numerous biological responses. sphingosine 1-phosphate 70-93 sphingosine kinase 1 Homo sapiens 0-18 20352614-1 2010 Sphingosine kinase (SphK) is a key enzyme in modulating the levels of sphingosine 1-phosphate (S1P) as well as an important enzyme in numerous biological responses. sphingosine 1-phosphate 70-93 sphingosine kinase 1 Homo sapiens 20-24 20352614-2 2010 Using C17-sphingosine as a substrate, we established a rapid, sensitive and highly efficient method for determination of SphK activity by analyzing the product C17-sphingosine 1-phosphate (C17-S1P) using liquid chromatography-tandem mass spectrometry. C17 Sphingosine 6-21 sphingosine kinase 1 Homo sapiens 121-125 20352614-2 2010 Using C17-sphingosine as a substrate, we established a rapid, sensitive and highly efficient method for determination of SphK activity by analyzing the product C17-sphingosine 1-phosphate (C17-S1P) using liquid chromatography-tandem mass spectrometry. C17 Sphingosine-1-phosphate 160-187 sphingosine kinase 1 Homo sapiens 121-125 20352614-7 2010 In contrast, the SphK activity decreased in cultured HEK 293 cells treated with dimethylsphngosine, a sphingosine kinase inhibitor. dimethylsphngosine 80-98 sphingosine kinase 1 Homo sapiens 17-21 20352614-7 2010 In contrast, the SphK activity decreased in cultured HEK 293 cells treated with dimethylsphngosine, a sphingosine kinase inhibitor. dimethylsphngosine 80-98 sphingosine kinase 1 Homo sapiens 102-120 20570726-0 2010 FTY720 and (S)-FTY720 vinylphosphonate inhibit sphingosine kinase 1 and promote its proteasomal degradation in human pulmonary artery smooth muscle, breast cancer and androgen-independent prostate cancer cells. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 11-38 sphingosine kinase 1 Homo sapiens 47-67 20570726-1 2010 Sphingosine kinase 1 (SK1) is an enzyme that catalyses the phosphorylation of sphingosine to produce the bioactive lipid sphingosine 1-phosphate (S1P). Sphingosine 78-89 sphingosine kinase 1 Homo sapiens 0-20 20570726-1 2010 Sphingosine kinase 1 (SK1) is an enzyme that catalyses the phosphorylation of sphingosine to produce the bioactive lipid sphingosine 1-phosphate (S1P). Sphingosine 78-89 sphingosine kinase 1 Homo sapiens 22-25 20570726-1 2010 Sphingosine kinase 1 (SK1) is an enzyme that catalyses the phosphorylation of sphingosine to produce the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 121-144 sphingosine kinase 1 Homo sapiens 0-20 20570726-1 2010 Sphingosine kinase 1 (SK1) is an enzyme that catalyses the phosphorylation of sphingosine to produce the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 121-144 sphingosine kinase 1 Homo sapiens 22-25 20570726-2 2010 We demonstrate here that FTY720 (Fingolimod) and (S)-FTY720 vinylphosphonate are novel inhibitors of SK1 catalytic activity and induce the proteasomal degradation of this enzyme in human pulmonary artery smooth muscle cells, MCF-7 breast cancer cells and androgen-independent LNCaP-AI prostate cancer cells. Fingolimod Hydrochloride 25-31 sphingosine kinase 1 Homo sapiens 101-104 20570726-2 2010 We demonstrate here that FTY720 (Fingolimod) and (S)-FTY720 vinylphosphonate are novel inhibitors of SK1 catalytic activity and induce the proteasomal degradation of this enzyme in human pulmonary artery smooth muscle cells, MCF-7 breast cancer cells and androgen-independent LNCaP-AI prostate cancer cells. Fingolimod Hydrochloride 33-43 sphingosine kinase 1 Homo sapiens 101-104 20570726-2 2010 We demonstrate here that FTY720 (Fingolimod) and (S)-FTY720 vinylphosphonate are novel inhibitors of SK1 catalytic activity and induce the proteasomal degradation of this enzyme in human pulmonary artery smooth muscle cells, MCF-7 breast cancer cells and androgen-independent LNCaP-AI prostate cancer cells. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 49-76 sphingosine kinase 1 Homo sapiens 101-104 20570726-3 2010 Proteasomal degradation of SK1 in response to FTY720 and (S)-FTY720 vinylphosphonate is associated with the down-regulation of the androgen receptor in LNCaP-AI cells. ((E)-3-amino-5-(4-heptylphenyl)-3-(hydroxymethyl)pent-1-enyl)phosphonic acid 57-84 sphingosine kinase 1 Homo sapiens 27-30 20522783-3 2010 We establish that SphK1 is a downstream effector of the ERK/phospholipase D (PLD) pathway, which is inhibited by green tea and wine polyphenols. Polyphenols 132-143 sphingosine kinase 1 Homo sapiens 18-23 20522783-4 2010 Enforced expression of SphK1 impaired the ability of green tea and wine polyphenols, as well as pharmacological inhibitors of PLD and ERK activities, to induce apoptosis in PC-3 and C4-2B cells. Polyphenols 72-83 sphingosine kinase 1 Homo sapiens 23-28 20522783-6 2010 PC-3/SphK1 cells implanted in animals developed larger tumors and resistance to treatment with polyphenols. Polyphenols 95-106 sphingosine kinase 1 Homo sapiens 5-10 20522783-7 2010 Furthermore, using an orthotopic PC-3/GFP model, the chemopreventive effect of an EGCg or PPE diet was associated with SphK1 inhibition, a decrease in primary tumor volume, and occurrence and number of metastases. epigallocatechin gallate 82-86 sphingosine kinase 1 Homo sapiens 119-124 20522783-8 2010 These results provide the first demonstration that the prosurvival, antiapoptotic SphK1/S1P pathway represents a target of dietary green tea and wine polyphenols in cancer. Polyphenols 150-161 sphingosine kinase 1 Homo sapiens 82-87 20804754-0 2010 Lysophosphatidic acid stimulates gastric cancer cell proliferation via ERK1-dependent upregulation of sphingosine kinase 1 transcription. lysophosphatidic acid 0-21 sphingosine kinase 1 Homo sapiens 102-122 20804754-1 2010 In MKN1 gastric cancer cells, lysophosphatidic acid (LPA) upregulates expression of sphingosine kinase 1 (SphK1) and its downregulation or inhibition suppresses LPA mediated proliferation. lysophosphatidic acid 30-51 sphingosine kinase 1 Homo sapiens 84-104 20804754-1 2010 In MKN1 gastric cancer cells, lysophosphatidic acid (LPA) upregulates expression of sphingosine kinase 1 (SphK1) and its downregulation or inhibition suppresses LPA mediated proliferation. lysophosphatidic acid 30-51 sphingosine kinase 1 Homo sapiens 106-111 20804754-1 2010 In MKN1 gastric cancer cells, lysophosphatidic acid (LPA) upregulates expression of sphingosine kinase 1 (SphK1) and its downregulation or inhibition suppresses LPA mediated proliferation. lysophosphatidic acid 53-56 sphingosine kinase 1 Homo sapiens 84-104 20804754-1 2010 In MKN1 gastric cancer cells, lysophosphatidic acid (LPA) upregulates expression of sphingosine kinase 1 (SphK1) and its downregulation or inhibition suppresses LPA mediated proliferation. lysophosphatidic acid 53-56 sphingosine kinase 1 Homo sapiens 106-111 20804754-1 2010 In MKN1 gastric cancer cells, lysophosphatidic acid (LPA) upregulates expression of sphingosine kinase 1 (SphK1) and its downregulation or inhibition suppresses LPA mediated proliferation. lysophosphatidic acid 161-164 sphingosine kinase 1 Homo sapiens 106-111 20804754-3 2010 Our data implicate ERK1 as an important mediator of LPA signaling leading to upregulation of SphK1 and point to SphK1 and sphingosine-1-phosphate production as potential therapeutic targets in gastric cancer. lysophosphatidic acid 52-55 sphingosine kinase 1 Homo sapiens 93-98 20520628-7 2010 Blockade of sphingosine kinase 1 (SPHK1) (but not SPHK2) by siRNA also increased apoptosis in L-UVB keratinocytes, revealing that conversion of sphingosine to sphingosine-1-phosphate (S1P) further protects keratinocytes from UVB-induced cell death. Sphingosine 12-23 sphingosine kinase 1 Homo sapiens 34-39 20520628-7 2010 Blockade of sphingosine kinase 1 (SPHK1) (but not SPHK2) by siRNA also increased apoptosis in L-UVB keratinocytes, revealing that conversion of sphingosine to sphingosine-1-phosphate (S1P) further protects keratinocytes from UVB-induced cell death. sphingosine 1-phosphate 159-182 sphingosine kinase 1 Homo sapiens 12-32 20520628-7 2010 Blockade of sphingosine kinase 1 (SPHK1) (but not SPHK2) by siRNA also increased apoptosis in L-UVB keratinocytes, revealing that conversion of sphingosine to sphingosine-1-phosphate (S1P) further protects keratinocytes from UVB-induced cell death. sphingosine 1-phosphate 159-182 sphingosine kinase 1 Homo sapiens 34-39 20403428-5 2010 Sphingosine kinase 1 reduces availability of sphingosine for interaction with 14-3-3, thus inhibiting cell death and providing a new mechanistic insight into the role of this enzyme in cell survival and oncogenesis. Sphingosine 45-56 sphingosine kinase 1 Homo sapiens 0-20 20386061-1 2010 Sphingosine kinase 1 (SK1) produces sphingosine-1-phosphate (S1P), a potent signaling lipid. sphingosine 1-phosphate 36-59 sphingosine kinase 1 Homo sapiens 0-25 20386061-4 2010 Expression of untargeted or ER-targeted SK1, but surprisingly not PM-targeted SK1, results in a dramatic increase in the phosphorylation of dihydrosphingosine, a metabolic precursor in de novo ceramide synthesis. safingol 140-158 sphingosine kinase 1 Homo sapiens 40-43 20386061-4 2010 Expression of untargeted or ER-targeted SK1, but surprisingly not PM-targeted SK1, results in a dramatic increase in the phosphorylation of dihydrosphingosine, a metabolic precursor in de novo ceramide synthesis. Ceramides 193-201 sphingosine kinase 1 Homo sapiens 40-43 20386061-5 2010 Conversely, knockdown of endogenous SK1 diminishes both dihydrosphingosine-1-phosphate and S1P levels. dihydrosphingosine 1-phosphate 56-86 sphingosine kinase 1 Homo sapiens 36-39 20518072-6 2010 We show in HeLa cells that NADH levels modulate the activities of two pivotal enzymes of sphingolipid metabolism: sphingosine kinase 1 (SK1) and neutral sphingomyelinase (nSMase). NAD 27-31 sphingosine kinase 1 Homo sapiens 114-134 20518072-6 2010 We show in HeLa cells that NADH levels modulate the activities of two pivotal enzymes of sphingolipid metabolism: sphingosine kinase 1 (SK1) and neutral sphingomyelinase (nSMase). NAD 27-31 sphingosine kinase 1 Homo sapiens 136-139 20518072-6 2010 We show in HeLa cells that NADH levels modulate the activities of two pivotal enzymes of sphingolipid metabolism: sphingosine kinase 1 (SK1) and neutral sphingomyelinase (nSMase). Sphingolipids 89-101 sphingosine kinase 1 Homo sapiens 114-134 20518072-6 2010 We show in HeLa cells that NADH levels modulate the activities of two pivotal enzymes of sphingolipid metabolism: sphingosine kinase 1 (SK1) and neutral sphingomyelinase (nSMase). Sphingolipids 89-101 sphingosine kinase 1 Homo sapiens 136-139 20518072-9 2010 Using plasma membranes isolated from cervical adenocarcinoma (HeLa) cells as well as purified proteins of both bacterial and human origin, we demonstrate that NADH inhibits SK1 and stimulates nSMase, while NAD(+) inhibits nSMase and has no effect on SK1. NAD 159-163 sphingosine kinase 1 Homo sapiens 173-176 20518072-9 2010 Using plasma membranes isolated from cervical adenocarcinoma (HeLa) cells as well as purified proteins of both bacterial and human origin, we demonstrate that NADH inhibits SK1 and stimulates nSMase, while NAD(+) inhibits nSMase and has no effect on SK1. NAD 159-163 sphingosine kinase 1 Homo sapiens 250-253 20649568-2 2010 EXPERIMENTAL APPROACH: We used the human endothelial cell line EA.hy926 to investigate the effect of nitric oxide (NO) donors on SK-1 expression, and on cell migration and tube formation. Nitric Oxide 101-113 sphingosine kinase 1 Homo sapiens 129-133 20649568-3 2010 KEY RESULTS: We showed that exposure of EA.hy926 cells to Deta-NO (125-1000 microM) resulted in a time- and concentration-dependent up-regulation of SK-1 mRNA and protein expression, and activity with a first significant effect at 250 microM of Deta-NO. DETA-NO 58-65 sphingosine kinase 1 Homo sapiens 149-153 20649568-3 2010 KEY RESULTS: We showed that exposure of EA.hy926 cells to Deta-NO (125-1000 microM) resulted in a time- and concentration-dependent up-regulation of SK-1 mRNA and protein expression, and activity with a first significant effect at 250 microM of Deta-NO. DETA-NO 245-252 sphingosine kinase 1 Homo sapiens 149-153 20649568-6 2010 In mechanistic terms, the NO-triggered effect occurred independently of cGMP, but involved the classical mitogen-activated protein kinase cascade because the MEK inhibitor U0126 abolished the NO-induced SK-1 expression. U 0126 172-177 sphingosine kinase 1 Homo sapiens 203-207 20404323-3 2010 In this report, we show that the production of sphingosine 1-phosphate (S1P) is significantly higher in A2780/HPR versus A2780 cells due to an increased sphingosine kinase (SK) activity and SK-1 mRNA and protein levels. sphingosine 1-phosphate 47-70 sphingosine kinase 1 Homo sapiens 190-194 20302564-4 2010 Sphingosine kinase-1 is a crucial regulator of this two-pan balance, because its produces the pro-survival and pro-angiogenic sphingosine 1-phosphate and decreases the amount of both ceramide and sphingosine, the pro-apoptotic sphingolipids. sphingosine 1-phosphate 126-149 sphingosine kinase 1 Homo sapiens 0-20 20156522-3 2010 SK catalyzes the phosphorylation of sphingosine to S1P and exists as two isoforms, SK1 and SK2. Sphingosine 36-47 sphingosine kinase 1 Homo sapiens 83-86 20302564-4 2010 Sphingosine kinase-1 is a crucial regulator of this two-pan balance, because its produces the pro-survival and pro-angiogenic sphingosine 1-phosphate and decreases the amount of both ceramide and sphingosine, the pro-apoptotic sphingolipids. Ceramides 183-191 sphingosine kinase 1 Homo sapiens 0-20 20302564-4 2010 Sphingosine kinase-1 is a crucial regulator of this two-pan balance, because its produces the pro-survival and pro-angiogenic sphingosine 1-phosphate and decreases the amount of both ceramide and sphingosine, the pro-apoptotic sphingolipids. Sphingosine 126-137 sphingosine kinase 1 Homo sapiens 0-20 20302564-4 2010 Sphingosine kinase-1 is a crucial regulator of this two-pan balance, because its produces the pro-survival and pro-angiogenic sphingosine 1-phosphate and decreases the amount of both ceramide and sphingosine, the pro-apoptotic sphingolipids. Sphingolipids 227-240 sphingosine kinase 1 Homo sapiens 0-20 20370685-1 2010 Sphingosine kinase (SK) 1 and 2 are lipid kinases that phosphorylate sphingosine to form sphingosine-1 phosphate, a potent signalling molecule with pleiotrophic effects. Sphingosine 69-80 sphingosine kinase 1 Homo sapiens 0-31 20370685-1 2010 Sphingosine kinase (SK) 1 and 2 are lipid kinases that phosphorylate sphingosine to form sphingosine-1 phosphate, a potent signalling molecule with pleiotrophic effects. sphingosine 1-phosphate 89-112 sphingosine kinase 1 Homo sapiens 0-31 20053797-5 2010 In this study, we tested the hypothesis that isoflurane, via TGF-beta1 release, increases caveolae formation in the buoyant fraction of the cell membrane of human renal proximal tubule (HK-2) cells to organize SK1 and S1P signaling. Isoflurane 45-55 sphingosine kinase 1 Homo sapiens 210-213 20179157-2 2010 Sphingosine kinase-1 and -2 (SK1 and SK2) phosphorylate sphingosine to form S1P, shifting the balanced activity of these lipids toward cell proliferation. Sphingosine 56-67 sphingosine kinase 1 Homo sapiens 0-27 20179157-2 2010 Sphingosine kinase-1 and -2 (SK1 and SK2) phosphorylate sphingosine to form S1P, shifting the balanced activity of these lipids toward cell proliferation. Sphingosine 56-67 sphingosine kinase 1 Homo sapiens 29-32 20577214-4 2010 TRAF2 binds to sphingosine kinase 1 (SphK1), one of the isoenzymes that generates the pro-survival lipid mediator sphingosine-1-phosphate (S1P) inside cells. sphingosine 1-phosphate 114-137 sphingosine kinase 1 Homo sapiens 15-35 20577214-4 2010 TRAF2 binds to sphingosine kinase 1 (SphK1), one of the isoenzymes that generates the pro-survival lipid mediator sphingosine-1-phosphate (S1P) inside cells. sphingosine 1-phosphate 114-137 sphingosine kinase 1 Homo sapiens 37-42 20577214-5 2010 Here we show that SphK1 and the production of S1P is necessary for lysine-63-linked polyubiquitination of RIP1, phosphorylation of IkappaB kinase and IkappaBalpha, and IkappaBalpha degradation, leading to NF-kappaB activation. Lysine 67-73 sphingosine kinase 1 Homo sapiens 18-23 20104488-0 2010 CXCL4-induced monocyte survival, cytokine expression, and oxygen radical formation is regulated by sphingosine kinase 1. Oxygen 58-64 sphingosine kinase 1 Homo sapiens 99-119 20236935-8 2010 Indirect immunofluorescence showed that phorbol ester, a potent protein kinase C activator that can also trigger acrosomal exocytosis, redistributes sphingosine kinase 1 to the acrosomal region. Phorbol Esters 40-53 sphingosine kinase 1 Homo sapiens 149-169 20236935-9 2010 Functional assays showed that phorbol ester-induced exocytosis depends on the activation of sphingosine kinase 1. Phorbol Esters 30-43 sphingosine kinase 1 Homo sapiens 92-112 20110355-1 2010 Sphingosine 1-phosphate (S1P), a potent sphingolipid mediator produced by sphingosine kinase isoenzymes (SphK1 and SphK2), regulates diverse cellular processes important for breast cancer progression acting in an autocrine and/or paracrine manner. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 105-110 20110355-1 2010 Sphingosine 1-phosphate (S1P), a potent sphingolipid mediator produced by sphingosine kinase isoenzymes (SphK1 and SphK2), regulates diverse cellular processes important for breast cancer progression acting in an autocrine and/or paracrine manner. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 105-110 20110355-1 2010 Sphingosine 1-phosphate (S1P), a potent sphingolipid mediator produced by sphingosine kinase isoenzymes (SphK1 and SphK2), regulates diverse cellular processes important for breast cancer progression acting in an autocrine and/or paracrine manner. Sphingolipids 40-52 sphingosine kinase 1 Homo sapiens 105-110 20104488-5 2010 Furthermore, we could show that pharmacological inhibition of SphK results in reversal of CXCL4-induced monocyte survival, cytokine expression, and release of oxygen radicals, which was confirmed by the use of SphK1-specific siRNA. Reactive Oxygen Species 159-174 sphingosine kinase 1 Homo sapiens 62-66 20104488-5 2010 Furthermore, we could show that pharmacological inhibition of SphK results in reversal of CXCL4-induced monocyte survival, cytokine expression, and release of oxygen radicals, which was confirmed by the use of SphK1-specific siRNA. Reactive Oxygen Species 159-174 sphingosine kinase 1 Homo sapiens 210-215 20502009-0 2010 A novel mode of action of the putative sphingosine kinase inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl) thiazole (SKI II): induction of lysosomal sphingosine kinase 1 degradation. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 68-116 sphingosine kinase 1 Homo sapiens 150-170 20153728-8 2010 Together, these data implicate Spred2 in imatinib-induced cytotoxicity in CML cells, possibly by inhibiting the Ras-ERK cascade and the pro-survival signaling molecules SPHK1 and Mcl-1. Imatinib Mesylate 41-49 sphingosine kinase 1 Homo sapiens 169-174 19914200-5 2010 Together the evidence indicates that the COOH-terminal region of SphK1 encompasses a structural element that is necessary for the increase in catalytic activity in response to PMA treatment and that its deletion renders SphK1 constitutively active with respect to PMA treatment. Tetradecanoylphorbol Acetate 264-267 sphingosine kinase 1 Homo sapiens 65-70 20234131-6 2010 Finally, overexpression of the SK1 enzyme in HK-2 cells protected against H(2)O(2)-induced necrosis. Hydrogen Peroxide 74-82 sphingosine kinase 1 Homo sapiens 31-34 20234131-7 2010 CONCLUSIONS: Collectively, our study demonstrates that S1P released via isoflurane-mediated SK1 stimulation produces direct anti-necrotic effects probably via S1P(1) receptor-mediated cytoprotective signaling (ERK/Akt phosphorylation and HSP70 induction) in HK-2 cells. Isoflurane 72-82 sphingosine kinase 1 Homo sapiens 92-95 20606403-1 2010 Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. Ceramides 131-139 sphingosine kinase 1 Homo sapiens 0-20 20606403-1 2010 Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. Ceramides 131-139 sphingosine kinase 1 Homo sapiens 22-27 20606403-1 2010 Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. sphingosine 1-phosphate 144-167 sphingosine kinase 1 Homo sapiens 0-20 20606403-1 2010 Sphingosine kinase-1 (SPHK1) modulates the proliferation, apoptosis and differentiation of keratinocytes through the regulation of ceramide and sphingosine-1-phosphate levels. sphingosine 1-phosphate 144-167 sphingosine kinase 1 Homo sapiens 22-27 19765607-10 2010 Over-expression of SphK1 promoted cell proliferation and protected ARPE-19 cells from ceramide-induced apoptosis. Ceramides 86-94 sphingosine kinase 1 Homo sapiens 19-24 19765607-15 2010 SphK1 over-expression increased cellular S1P, which promoted cell proliferation and protected RPE from ceramide-induced apoptosis. Ceramides 103-111 sphingosine kinase 1 Homo sapiens 0-5 20007411-4 2010 In contrast, induction of prostaglandin endoperoxide synthase 2 by S1P and stimulation of SPHK1 by estradiol and cytokines suggests a role for S1P in the termination of pregnancy. Estradiol 99-108 sphingosine kinase 1 Homo sapiens 90-95 19914200-3 2010 Herein, we report that deletion of 21 amino acids from the COOH-terminus of SphK1 (1-363) results in increased catalytic activity relative to wild-type SphK1 (1-384) which is independent of the phosphorylation state of Serine 225 and PMA stimulation. Serine 219-225 sphingosine kinase 1 Homo sapiens 76-81 19914200-3 2010 Herein, we report that deletion of 21 amino acids from the COOH-terminus of SphK1 (1-363) results in increased catalytic activity relative to wild-type SphK1 (1-384) which is independent of the phosphorylation state of Serine 225 and PMA stimulation. Tetradecanoylphorbol Acetate 234-237 sphingosine kinase 1 Homo sapiens 76-81 19914200-5 2010 Together the evidence indicates that the COOH-terminal region of SphK1 encompasses a structural element that is necessary for the increase in catalytic activity in response to PMA treatment and that its deletion renders SphK1 constitutively active with respect to PMA treatment. Tetradecanoylphorbol Acetate 176-179 sphingosine kinase 1 Homo sapiens 65-70 20502009-1 2010 BACKGROUND: Sphingosine kinase 1 (SK1) is a key enzyme in the generation of sphingosine 1-phosphate (S1P) which critically regulates a variety of important cell responses such as proliferation and migration. sphingosine 1-phosphate 76-99 sphingosine kinase 1 Homo sapiens 12-32 20502009-1 2010 BACKGROUND: Sphingosine kinase 1 (SK1) is a key enzyme in the generation of sphingosine 1-phosphate (S1P) which critically regulates a variety of important cell responses such as proliferation and migration. sphingosine 1-phosphate 76-99 sphingosine kinase 1 Homo sapiens 34-37 20502009-3 2010 RESULTS: We show here that the previously developed putative SK-1 inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl) thiazole (SKI II) displays an additional facet of action complementary to the known inhibition of enzymatic SK-1 activity. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 76-124 sphingosine kinase 1 Homo sapiens 61-65 20502009-3 2010 RESULTS: We show here that the previously developed putative SK-1 inhibitor 2-(p-hydroxyanilino)-4-(p-chlorophenyl) thiazole (SKI II) displays an additional facet of action complementary to the known inhibition of enzymatic SK-1 activity. 4-(4-(4-chloro-phenyl)thiazol-2-ylamino)phenol 76-124 sphingosine kinase 1 Homo sapiens 224-228 20502009-5 2010 By using cycloheximide to block the de novo protein synthesis, the protein expression of SK-1 under untreated conditions was stable over 24h. Cycloheximide 9-22 sphingosine kinase 1 Homo sapiens 89-93 19854831-9 2010 Furthermore, we demonstrate the requirement of CIB1-mediated translocation of SK1 in controlling cellular sphingosine 1-phosphate generation and associated anti-apoptotic signaling. sphingosine 1-phosphate 106-129 sphingosine kinase 1 Homo sapiens 78-81 19521959-0 2009 Sphingosine kinase 1 inhibition sensitizes hormone-resistant prostate cancer to docetaxel. Docetaxel 80-89 sphingosine kinase 1 Homo sapiens 0-20 19617631-8 2010 Interestingly, the mRNA level of enzymes involved in synthesis and degradation of ceramide including serine palmitoyltransferase, sphingosine kinase 1, neutral sphingomyelinase, and ceramidases was markedly higher in the myocardium of OWT and T2D-OWT patients compared with lean subjects. Ceramides 82-90 sphingosine kinase 1 Homo sapiens 130-150 20508814-2 2010 This effect is probably due to the ability of SphK-1 to be released into the extracellular medium where it catalyzes the biosynthesis of sphingosine-1-phosphate (S1P), a signaling molecule endowed with profound proangiogenic effects. sphingosine 1-phosphate 137-160 sphingosine kinase 1 Homo sapiens 46-52 19782042-3 2009 In this report, using multiple biochemical and subcellular fractionation techniques we demonstrate that endogenous SphK1 protein and its substrate, D-erythro-sphingosine, are present within the PMLRM. Sphingosine 148-169 sphingosine kinase 1 Homo sapiens 115-120 19782042-4 2009 Additionally, we demonstrate that the PMA stimulation of SphK1 localized to the PMLRM results in production of sphingosine-1-phosphate as well as induction of cell growth under serum deprivation conditions. sphingosine 1-phosphate 111-134 sphingosine kinase 1 Homo sapiens 57-62 19521959-5 2009 We have shown that SphK1 inhibition by docetaxel is a two-step process involving an initial loss of enzyme activity followed by a decrease in SphK1 gene expression. Docetaxel 39-48 sphingosine kinase 1 Homo sapiens 19-24 19521959-5 2009 We have shown that SphK1 inhibition by docetaxel is a two-step process involving an initial loss of enzyme activity followed by a decrease in SphK1 gene expression. Docetaxel 39-48 sphingosine kinase 1 Homo sapiens 142-147 19521959-6 2009 Using hormoneresistant PC-3 and DU145 PCa cells we have demonstrated that both pharmacological and siRNA-mediated SphK1 inhibition leads to a four-fold decrease in the docetaxel IC50 dose. Docetaxel 168-177 sphingosine kinase 1 Homo sapiens 114-119 19543831-0 2009 Decrease of Tau hyperphosphorylation by 17beta estradiol requires sphingosine kinase in a glutamate toxicity model. Estradiol 40-56 sphingosine kinase 1 Homo sapiens 66-84 19543831-0 2009 Decrease of Tau hyperphosphorylation by 17beta estradiol requires sphingosine kinase in a glutamate toxicity model. Glutamic Acid 90-99 sphingosine kinase 1 Homo sapiens 66-84 19543831-1 2009 Several studies have linked estrogens with sphingosine kinase (SphK) activity, enzyme responsible of sphingosine-1-phosphate synthesis (S-1P), however their possible interaction in the nervous system is not documented yet. sphingosine 1-phosphate 101-124 sphingosine kinase 1 Homo sapiens 43-61 19543831-1 2009 Several studies have linked estrogens with sphingosine kinase (SphK) activity, enzyme responsible of sphingosine-1-phosphate synthesis (S-1P), however their possible interaction in the nervous system is not documented yet. sphingosine 1-phosphate 101-124 sphingosine kinase 1 Homo sapiens 63-67 19543831-2 2009 In the present study, we developed a glutamate toxicity model in SH-SY5Y cells to evaluate the possible effect of the inhibition of SphK activity on the protective capability of 17beta-estradiol (E2). Estradiol 178-194 sphingosine kinase 1 Homo sapiens 132-136 19543831-4 2009 These effects were prevented by E2 pre-treatment during 24 h. Although the inhibition of SphK did not block this protective effect, significantly increased Tau hyperphosphorylation by glutamate, in a way that was not reverted by E2. Glutamic Acid 184-193 sphingosine kinase 1 Homo sapiens 89-93 19543831-5 2009 Our results suggest that the decrease of glutamate-induced Tau hyperphosphorylation by 17beta-estradiol requires SphK. Glutamic Acid 41-50 sphingosine kinase 1 Homo sapiens 113-117 19543831-5 2009 Our results suggest that the decrease of glutamate-induced Tau hyperphosphorylation by 17beta-estradiol requires SphK. Estradiol 87-103 sphingosine kinase 1 Homo sapiens 113-117 19956567-6 2009 Similarly, the addition of dihydrotestosterone (DHT) to androgen-deprived LNCaP cells re-established cell proliferation, through an androgen receptor/PI3K/Akt dependent stimulation of SphK1, and inhibition of SphK1 could markedly impede the effects of DHT. Dihydrotestosterone 27-46 sphingosine kinase 1 Homo sapiens 184-189 19956567-6 2009 Similarly, the addition of dihydrotestosterone (DHT) to androgen-deprived LNCaP cells re-established cell proliferation, through an androgen receptor/PI3K/Akt dependent stimulation of SphK1, and inhibition of SphK1 could markedly impede the effects of DHT. Dihydrotestosterone 27-46 sphingosine kinase 1 Homo sapiens 209-214 19956567-6 2009 Similarly, the addition of dihydrotestosterone (DHT) to androgen-deprived LNCaP cells re-established cell proliferation, through an androgen receptor/PI3K/Akt dependent stimulation of SphK1, and inhibition of SphK1 could markedly impede the effects of DHT. Dihydrotestosterone 48-51 sphingosine kinase 1 Homo sapiens 184-189 19956567-6 2009 Similarly, the addition of dihydrotestosterone (DHT) to androgen-deprived LNCaP cells re-established cell proliferation, through an androgen receptor/PI3K/Akt dependent stimulation of SphK1, and inhibition of SphK1 could markedly impede the effects of DHT. Dihydrotestosterone 48-51 sphingosine kinase 1 Homo sapiens 209-214 19521959-3 2009 We have previously reported that sphingosine kinase 1 (SphK1) inhibition is a key step in docetaxel-induced apoptosis in the PC-3 PCa cell line and that pharmacologicalSphK1 inhibition is chemosensitizing in the docetaxel-resistant PCa LNCaP cell line. Docetaxel 90-99 sphingosine kinase 1 Homo sapiens 33-53 19521959-3 2009 We have previously reported that sphingosine kinase 1 (SphK1) inhibition is a key step in docetaxel-induced apoptosis in the PC-3 PCa cell line and that pharmacologicalSphK1 inhibition is chemosensitizing in the docetaxel-resistant PCa LNCaP cell line. Docetaxel 90-99 sphingosine kinase 1 Homo sapiens 55-60 19521959-3 2009 We have previously reported that sphingosine kinase 1 (SphK1) inhibition is a key step in docetaxel-induced apoptosis in the PC-3 PCa cell line and that pharmacologicalSphK1 inhibition is chemosensitizing in the docetaxel-resistant PCa LNCaP cell line. Docetaxel 212-221 sphingosine kinase 1 Homo sapiens 33-53 19521959-3 2009 We have previously reported that sphingosine kinase 1 (SphK1) inhibition is a key step in docetaxel-induced apoptosis in the PC-3 PCa cell line and that pharmacologicalSphK1 inhibition is chemosensitizing in the docetaxel-resistant PCa LNCaP cell line. Docetaxel 212-221 sphingosine kinase 1 Homo sapiens 55-60 19521959-4 2009 In this study we have addressed the mechanism of docetaxel-induced apoptosis of PC-3 cells and identified SphK1-dependent and -independent components. Docetaxel 49-58 sphingosine kinase 1 Homo sapiens 106-111 19800228-1 2009 Sphingosine kinase 1 (SK1) is an important enzyme that regulates the balance between ceramide and sphingosine-1-phosphate (S1P). Ceramides 85-93 sphingosine kinase 1 Homo sapiens 0-20 19815712-5 2009 Overexpression studies demonstrate a requirement for SK-1 phosphorylation at serine 225 for increased heterotrimeric complex formation, activation of alpha(v)beta(3), and EC survival signals, including Bcl-X and nuclear factor-kappaB pathways. Serine 77-83 sphingosine kinase 1 Homo sapiens 53-57 19800228-1 2009 Sphingosine kinase 1 (SK1) is an important enzyme that regulates the balance between ceramide and sphingosine-1-phosphate (S1P). Ceramides 85-93 sphingosine kinase 1 Homo sapiens 22-25 19800228-1 2009 Sphingosine kinase 1 (SK1) is an important enzyme that regulates the balance between ceramide and sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 98-121 sphingosine kinase 1 Homo sapiens 0-20 19800228-1 2009 Sphingosine kinase 1 (SK1) is an important enzyme that regulates the balance between ceramide and sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 98-121 sphingosine kinase 1 Homo sapiens 22-25 19800228-2 2009 Potent and novel SK1 inhibitors (6ag, 9ab and 12aa) have been discovered through a series of modifications of sphingosine (1), the substrate of this enzyme. Sphingosine 110-121 sphingosine kinase 1 Homo sapiens 17-20 19219548-6 2009 Herein, we show that in human endothelial cells, VEGF-induced angiogenesis is mitigated by dimethylsphingosine and suramin; inhibitors of sphingosine kinase 1(SphK-1) and sphingosine1-phosphate receptor 1(S1P (1)), respectively, and this were bypassed by LacCer but not by S1P. dimethylsphingosine 91-110 sphingosine kinase 1 Homo sapiens 138-158 19842132-4 2009 The L-ribo diols 8 and 16, and the amino alcohols 19 and 20 inhibit sphingosine kinase 1 (SPHK1), while the L-arabino analogues 7, 15, 17, and 18 are inactive. l-ribo diols 4-16 sphingosine kinase 1 Homo sapiens 90-95 19842132-4 2009 The L-ribo diols 8 and 16, and the amino alcohols 19 and 20 inhibit sphingosine kinase 1 (SPHK1), while the L-arabino analogues 7, 15, 17, and 18 are inactive. Amino Alcohols 35-49 sphingosine kinase 1 Homo sapiens 68-88 19842132-4 2009 The L-ribo diols 8 and 16, and the amino alcohols 19 and 20 inhibit sphingosine kinase 1 (SPHK1), while the L-arabino analogues 7, 15, 17, and 18 are inactive. Amino Alcohols 35-49 sphingosine kinase 1 Homo sapiens 90-95 19657322-5 2009 Over-expression of SK-1 reduced CTGF induction, an effect mediated by intracellular sphingosine-1-phosphate. sphingosine 1-phosphate 84-107 sphingosine kinase 1 Homo sapiens 19-23 19723667-2 2009 Sphingosine kinase 1 (SphK1), one of the two isoenzymes that produce sphingosine-1-phosphate, is up-regulated in glioblastoma and has been linked to poor prognosis in patients with glioblastoma multiforme (GBM). sphingosine 1-phosphate 69-92 sphingosine kinase 1 Homo sapiens 0-20 19723667-2 2009 Sphingosine kinase 1 (SphK1), one of the two isoenzymes that produce sphingosine-1-phosphate, is up-regulated in glioblastoma and has been linked to poor prognosis in patients with glioblastoma multiforme (GBM). sphingosine 1-phosphate 69-92 sphingosine kinase 1 Homo sapiens 22-27 19515965-5 2009 In addition to this positive modulation, GW542573X activated SK1 in the absence of Ca(2+) and furthermore induced a 15% increase in the maximal current at saturating Ca(2+). 4-(2-methoxyphenylcarbamoyloxymethyl)piperidine-1-carboxylic acid tert-butyl ester 41-50 sphingosine kinase 1 Homo sapiens 61-64 19515965-6 2009 Thus, GW542573X also acts as a genuine opener of the hSK1 channels, a mechanism of action (MOA) not previously obtained with SK channels. 4-(2-methoxyphenylcarbamoyloxymethyl)piperidine-1-carboxylic acid tert-butyl ester 6-15 sphingosine kinase 1 Homo sapiens 53-57 19515965-8 2009 A single amino acid (Ser293) located in S5 of hSK1 was essential, and substituting the corresponding Leu476 in hSK3 with serine conferred hSK1-like potency (EC(50) = 9.3 +/- 1.4 microM, n = 5). Serine 121-127 sphingosine kinase 1 Homo sapiens 46-50 19515965-8 2009 A single amino acid (Ser293) located in S5 of hSK1 was essential, and substituting the corresponding Leu476 in hSK3 with serine conferred hSK1-like potency (EC(50) = 9.3 +/- 1.4 microM, n = 5). Serine 121-127 sphingosine kinase 1 Homo sapiens 138-142 19706837-3 2009 Enforced overexpression of human SphK1 in MCF-7 cells resulted in enhanced cell proliferation and resistance to tamoxifen-induced cell growth arrest and apoptosis. Tamoxifen 112-121 sphingosine kinase 1 Homo sapiens 33-38 19706837-4 2009 Tamoxifen-resistant (TamR) MCF-7 cells selected by prolonged exposure to 4-hydroxytamoxifen, exhibited higher levels in SphK1 expression and activity, compared with the control cells. Tamoxifen 0-9 sphingosine kinase 1 Homo sapiens 120-125 19706837-4 2009 Tamoxifen-resistant (TamR) MCF-7 cells selected by prolonged exposure to 4-hydroxytamoxifen, exhibited higher levels in SphK1 expression and activity, compared with the control cells. tamr 21-25 sphingosine kinase 1 Homo sapiens 120-125 19706837-4 2009 Tamoxifen-resistant (TamR) MCF-7 cells selected by prolonged exposure to 4-hydroxytamoxifen, exhibited higher levels in SphK1 expression and activity, compared with the control cells. hydroxytamoxifen 73-91 sphingosine kinase 1 Homo sapiens 120-125 19706837-5 2009 Inhibition of SphK1 activity by either specific pharmaceutical inhibitors or the dominant-negative mutant SphK1(G82D) restored the antiproliferative and proapoptotic effects of tamoxifen in the TamR cells. Tamoxifen 177-186 sphingosine kinase 1 Homo sapiens 14-19 19706837-5 2009 Inhibition of SphK1 activity by either specific pharmaceutical inhibitors or the dominant-negative mutant SphK1(G82D) restored the antiproliferative and proapoptotic effects of tamoxifen in the TamR cells. Tamoxifen 177-186 sphingosine kinase 1 Homo sapiens 106-111 19706837-6 2009 Furthermore, silencing of SphK1, but not SphK2, expression by the specific small interference RNA also restored the tamoxifen responsiveness in the TamR cells. Tamoxifen 116-125 sphingosine kinase 1 Homo sapiens 26-31 19706837-7 2009 Thus, blockade of the SphK1 signaling pathway may reprogram cellular responsiveness to tamoxifen and abrogate antiestrogen resistance in human breast cancer cells. Tamoxifen 87-96 sphingosine kinase 1 Homo sapiens 22-27 19515965-0 2009 Selective activation of the SK1 subtype of human small-conductance Ca2+-activated K+ channels by 4-(2-methoxyphenylcarbamoyloxymethyl)-piperidine-1-carboxylic acid tert-butyl ester (GW542573X) is dependent on serine 293 in the S5 segment. 4-(2-methoxyphenylcarbamoyloxymethyl)piperidine-1-carboxylic acid tert-butyl ester 97-180 sphingosine kinase 1 Homo sapiens 28-31 19515965-0 2009 Selective activation of the SK1 subtype of human small-conductance Ca2+-activated K+ channels by 4-(2-methoxyphenylcarbamoyloxymethyl)-piperidine-1-carboxylic acid tert-butyl ester (GW542573X) is dependent on serine 293 in the S5 segment. 4-(2-methoxyphenylcarbamoyloxymethyl)piperidine-1-carboxylic acid tert-butyl ester 182-191 sphingosine kinase 1 Homo sapiens 28-31 19515965-0 2009 Selective activation of the SK1 subtype of human small-conductance Ca2+-activated K+ channels by 4-(2-methoxyphenylcarbamoyloxymethyl)-piperidine-1-carboxylic acid tert-butyl ester (GW542573X) is dependent on serine 293 in the S5 segment. Serine 209-215 sphingosine kinase 1 Homo sapiens 28-31 19515965-2 2009 GW542573X is the first SK1-selective compound described: an EC(50) value of 8.2 +/- 0.8 microM (n = 6, [Ca(2+)](i) = 200 nM) was obtained from inside-out patches excised from hSK1-expressing HEK293 cells. 4-(2-methoxyphenylcarbamoyloxymethyl)piperidine-1-carboxylic acid tert-butyl ester 0-9 sphingosine kinase 1 Homo sapiens 23-26 19515965-2 2009 GW542573X is the first SK1-selective compound described: an EC(50) value of 8.2 +/- 0.8 microM (n = 6, [Ca(2+)](i) = 200 nM) was obtained from inside-out patches excised from hSK1-expressing HEK293 cells. 4-(2-methoxyphenylcarbamoyloxymethyl)piperidine-1-carboxylic acid tert-butyl ester 0-9 sphingosine kinase 1 Homo sapiens 175-179 19515965-4 2009 The Ca(2+)-response curve of hSK1 was left-shifted from an EC(50)(Ca(2+)) value of 410 +/- 20 nM (n = 9) to 240 +/- 10 nM (n = 5) in the presence of 10 microM GW542573X. 4-(2-methoxyphenylcarbamoyloxymethyl)piperidine-1-carboxylic acid tert-butyl ester 159-168 sphingosine kinase 1 Homo sapiens 29-33 19609004-9 2009 To enhance the cytotoxic effect R-MA, sphingosine kinase-1 and glucosylceramide synthase, enzymes that convert ceramide to the pro-proliferative sphingolipids sphingosine-1-phospate and glucosylceramide, respectively, were inhibited. Sphingolipids 145-158 sphingosine kinase 1 Homo sapiens 38-88 19609004-9 2009 To enhance the cytotoxic effect R-MA, sphingosine kinase-1 and glucosylceramide synthase, enzymes that convert ceramide to the pro-proliferative sphingolipids sphingosine-1-phospate and glucosylceramide, respectively, were inhibited. sphingosine-1-phospate 159-181 sphingosine kinase 1 Homo sapiens 38-88 19219548-6 2009 Herein, we show that in human endothelial cells, VEGF-induced angiogenesis is mitigated by dimethylsphingosine and suramin; inhibitors of sphingosine kinase 1(SphK-1) and sphingosine1-phosphate receptor 1(S1P (1)), respectively, and this were bypassed by LacCer but not by S1P. dimethylsphingosine 91-110 sphingosine kinase 1 Homo sapiens 159-165 19219548-6 2009 Herein, we show that in human endothelial cells, VEGF-induced angiogenesis is mitigated by dimethylsphingosine and suramin; inhibitors of sphingosine kinase 1(SphK-1) and sphingosine1-phosphate receptor 1(S1P (1)), respectively, and this were bypassed by LacCer but not by S1P. Suramin 115-122 sphingosine kinase 1 Homo sapiens 138-158 19293152-2 2009 Sphingosine kinase (SphK) contributes to these functions by converting sphingosine to S1P. Sphingosine 71-82 sphingosine kinase 1 Homo sapiens 0-18 19506243-7 2009 The molecular mechanisms triggering these responses include the activation of phospholipase D1 and sphingosine kinase1 to mediate calcium mobilization, Nuclear factor-kappaB activation, cytokine and eicosanoid secretion, and degranulation. Calcium 130-137 sphingosine kinase 1 Homo sapiens 99-118 19325137-4 2009 In the angiogenic signaling, we focused on sphingosine kinase-1 (SK1), which produces sphingosine-1-phosphate (S1P), a bioactive sphingolipid, as a potent angiogenic mediator in ECs. sphingosine 1-phosphate 86-109 sphingosine kinase 1 Homo sapiens 43-68 19325137-4 2009 In the angiogenic signaling, we focused on sphingosine kinase-1 (SK1), which produces sphingosine-1-phosphate (S1P), a bioactive sphingolipid, as a potent angiogenic mediator in ECs. sphingosine 1-phosphate 111-114 sphingosine kinase 1 Homo sapiens 43-68 19325137-4 2009 In the angiogenic signaling, we focused on sphingosine kinase-1 (SK1), which produces sphingosine-1-phosphate (S1P), a bioactive sphingolipid, as a potent angiogenic mediator in ECs. Sphingolipids 129-141 sphingosine kinase 1 Homo sapiens 43-68 19293152-2 2009 Sphingosine kinase (SphK) contributes to these functions by converting sphingosine to S1P. Sphingosine 71-82 sphingosine kinase 1 Homo sapiens 20-24 19293152-2 2009 Sphingosine kinase (SphK) contributes to these functions by converting sphingosine to S1P. sphingosine 1-phosphate 86-89 sphingosine kinase 1 Homo sapiens 0-18 19293152-2 2009 Sphingosine kinase (SphK) contributes to these functions by converting sphingosine to S1P. sphingosine 1-phosphate 86-89 sphingosine kinase 1 Homo sapiens 20-24 19070545-1 2009 Sphingosine-1-phosphate (S1P) is a bioactive lysophospholipid generated by the sphingosine kinase (SK1 or SK2)-catalysed phosphorylation of sphingosine. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 99-102 19211033-1 2009 We show here that the endogenous sphingosine 1-phosphate 5 receptor (S1P(5), a G protein coupled receptor (GPCR) whose natural ligand is sphingosine 1-phosphate (S1P)) and sphingosine kinases 1 and 2 (SK1 and SK2), which catalyse formation of S1P, are co-localised in the centrosome of mammalian cells, where they may participate in regulating mitosis. sphingosine 1-phosphate 33-56 sphingosine kinase 1 Homo sapiens 201-204 19479838-3 2009 The 7-thiasphingosine 5 is a poorer substrate for both isoforms of sphingosine kinase (SPHK) than sphingosine, but showed a slight preference for SPHK2. 7-thiasphingosine 4-21 sphingosine kinase 1 Homo sapiens 67-85 19479838-3 2009 The 7-thiasphingosine 5 is a poorer substrate for both isoforms of sphingosine kinase (SPHK) than sphingosine, but showed a slight preference for SPHK2. 7-thiasphingosine 4-21 sphingosine kinase 1 Homo sapiens 87-91 19479838-3 2009 The 7-thiasphingosine 5 is a poorer substrate for both isoforms of sphingosine kinase (SPHK) than sphingosine, but showed a slight preference for SPHK2. Sphingosine 10-21 sphingosine kinase 1 Homo sapiens 67-85 19479838-3 2009 The 7-thiasphingosine 5 is a poorer substrate for both isoforms of sphingosine kinase (SPHK) than sphingosine, but showed a slight preference for SPHK2. Sphingosine 10-21 sphingosine kinase 1 Homo sapiens 87-91 19240026-0 2009 Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and Akt activation. Oxaliplatin 37-48 sphingosine kinase 1 Homo sapiens 0-18 19240026-0 2009 Sphingosine kinase isoforms regulate oxaliplatin sensitivity of human colon cancer cells through ceramide accumulation and Akt activation. Ceramides 97-105 sphingosine kinase 1 Homo sapiens 0-18 19240026-6 2009 In contrast, in l-OHP-resistant RKO cells, treatment with an SPHK inhibitor or SPHK1 and SPHK2 silencing by RNA interference suppressed cell viability and increased caspase activity and cellular ceramide formation after l-OHP treatment. Oxaliplatin 16-21 sphingosine kinase 1 Homo sapiens 61-65 19240026-6 2009 In contrast, in l-OHP-resistant RKO cells, treatment with an SPHK inhibitor or SPHK1 and SPHK2 silencing by RNA interference suppressed cell viability and increased caspase activity and cellular ceramide formation after l-OHP treatment. Oxaliplatin 16-21 sphingosine kinase 1 Homo sapiens 79-84 19240026-6 2009 In contrast, in l-OHP-resistant RKO cells, treatment with an SPHK inhibitor or SPHK1 and SPHK2 silencing by RNA interference suppressed cell viability and increased caspase activity and cellular ceramide formation after l-OHP treatment. Ceramides 195-203 sphingosine kinase 1 Homo sapiens 61-65 19240026-6 2009 In contrast, in l-OHP-resistant RKO cells, treatment with an SPHK inhibitor or SPHK1 and SPHK2 silencing by RNA interference suppressed cell viability and increased caspase activity and cellular ceramide formation after l-OHP treatment. Ceramides 195-203 sphingosine kinase 1 Homo sapiens 79-84 19240026-6 2009 In contrast, in l-OHP-resistant RKO cells, treatment with an SPHK inhibitor or SPHK1 and SPHK2 silencing by RNA interference suppressed cell viability and increased caspase activity and cellular ceramide formation after l-OHP treatment. Oxaliplatin 220-225 sphingosine kinase 1 Homo sapiens 61-65 19240026-6 2009 In contrast, in l-OHP-resistant RKO cells, treatment with an SPHK inhibitor or SPHK1 and SPHK2 silencing by RNA interference suppressed cell viability and increased caspase activity and cellular ceramide formation after l-OHP treatment. Oxaliplatin 220-225 sphingosine kinase 1 Homo sapiens 79-84 19240026-7 2009 The elevated ceramide formation induced by SPHK inhibition and l-OHP was inhibited by fumonisin B1 but not myriocin, suggesting that ceramide formation was through the salvage pathway. Ceramides 13-21 sphingosine kinase 1 Homo sapiens 43-47 19240026-7 2009 The elevated ceramide formation induced by SPHK inhibition and l-OHP was inhibited by fumonisin B1 but not myriocin, suggesting that ceramide formation was through the salvage pathway. fumonisin B1 86-98 sphingosine kinase 1 Homo sapiens 43-47 19240026-7 2009 The elevated ceramide formation induced by SPHK inhibition and l-OHP was inhibited by fumonisin B1 but not myriocin, suggesting that ceramide formation was through the salvage pathway. Ceramides 133-141 sphingosine kinase 1 Homo sapiens 43-47 19240026-9 2009 Either SPHK1 or SPHK2 silencing in RKO cells decreased phosphorylated Akt levels and increased p53 and p21 protein levels as well as poly(ADP-ribose) polymerase cleavage in response to l-OHP treatment. Oxaliplatin 185-190 sphingosine kinase 1 Homo sapiens 7-12 19240026-10 2009 These findings indicate that SPHK isoforms and neutral sphingomyelinase contribute to the regulation of chemosensitivity by controlling ceramide formation and the downstream Akt pathway in human colon cancer cells. Ceramides 136-144 sphingosine kinase 1 Homo sapiens 29-33 19168577-1 2009 Two mammalian sphingosine kinase (SphK) isoforms, SphK1 and SphK2, possess identical kinase domains but have distinct kinetic properties and subcellular localizations, suggesting each has one or more specific roles in sphingosine-1-phosphate (S1P) generation. sphingosine 1-phosphate 218-241 sphingosine kinase 1 Homo sapiens 14-32 19168577-1 2009 Two mammalian sphingosine kinase (SphK) isoforms, SphK1 and SphK2, possess identical kinase domains but have distinct kinetic properties and subcellular localizations, suggesting each has one or more specific roles in sphingosine-1-phosphate (S1P) generation. sphingosine 1-phosphate 218-241 sphingosine kinase 1 Homo sapiens 34-38 19168577-1 2009 Two mammalian sphingosine kinase (SphK) isoforms, SphK1 and SphK2, possess identical kinase domains but have distinct kinetic properties and subcellular localizations, suggesting each has one or more specific roles in sphingosine-1-phosphate (S1P) generation. sphingosine 1-phosphate 218-241 sphingosine kinase 1 Homo sapiens 50-55 19372554-6 2009 A low level of the ceramide/S1P ratio, associated with a high SphK1 activity, correlates with a robust intrinsic pancreatic cancer cell chemoresistance toward gemcitabine. Ceramides 19-27 sphingosine kinase 1 Homo sapiens 62-67 19372554-6 2009 A low level of the ceramide/S1P ratio, associated with a high SphK1 activity, correlates with a robust intrinsic pancreatic cancer cell chemoresistance toward gemcitabine. gemcitabine 159-170 sphingosine kinase 1 Homo sapiens 62-67 19372554-7 2009 Strikingly, increasing the ceramide/S1P ratio, by using pharmacologic (SphK1 inhibitor or ceramide analogue) or small interfering RNA-based approaches to up-regulate intracellular ceramide levels or reduce SphK1 activity, sensitized pancreatic cancer cells to gemcitabine. Ceramides 27-35 sphingosine kinase 1 Homo sapiens 71-76 19372554-7 2009 Strikingly, increasing the ceramide/S1P ratio, by using pharmacologic (SphK1 inhibitor or ceramide analogue) or small interfering RNA-based approaches to up-regulate intracellular ceramide levels or reduce SphK1 activity, sensitized pancreatic cancer cells to gemcitabine. Ceramides 27-35 sphingosine kinase 1 Homo sapiens 206-211 19372554-8 2009 Conversely, decreasing the ceramide/S1P ratio, by up-regulating SphK1 activity, promoted gemcitabine resistance in these cells. Ceramides 27-35 sphingosine kinase 1 Homo sapiens 64-69 19372554-8 2009 Conversely, decreasing the ceramide/S1P ratio, by up-regulating SphK1 activity, promoted gemcitabine resistance in these cells. gemcitabine 89-100 sphingosine kinase 1 Homo sapiens 64-69 19119142-7 2009 FTY720-induced up-regulation of DHS1P level was mediated by sphingosine kinase (SphK) 1, but not SphK2, as confirmed by experiments using SphK1/2 silencing with small interfering RNA. dihydrosphingosine 1-phosphate 32-37 sphingosine kinase 1 Homo sapiens 60-87 19119142-7 2009 FTY720-induced up-regulation of DHS1P level was mediated by sphingosine kinase (SphK) 1, but not SphK2, as confirmed by experiments using SphK1/2 silencing with small interfering RNA. dihydrosphingosine 1-phosphate 32-37 sphingosine kinase 1 Homo sapiens 138-143 19238330-1 2009 Sphingosine kinase-1 (SK1) promotes the formation of sphingosine-1-phosphate (S1P), which has potent pro-inflammatory and pro-angiogenic effects. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 0-25 19238330-1 2009 Sphingosine kinase-1 (SK1) promotes the formation of sphingosine-1-phosphate (S1P), which has potent pro-inflammatory and pro-angiogenic effects. sphingosine 1-phosphate 78-81 sphingosine kinase 1 Homo sapiens 0-25 19238331-1 2009 The enzyme sphingosine kinase-1 (SK1) promotes the formation of sphingosine-1-phosphate (S1P), which is an important survival factor for endothelial cells (EC). sphingosine 1-phosphate 64-87 sphingosine kinase 1 Homo sapiens 11-36 19167412-3 2009 Sphingosine kinase (SphK), a key enzyme in metabolic pathways of sphingolipids, plays an important role in cell signaling and regulates a wide range of biological functions, including the actions of estrogens. Sphingolipids 65-78 sphingosine kinase 1 Homo sapiens 0-18 19167412-3 2009 Sphingosine kinase (SphK), a key enzyme in metabolic pathways of sphingolipids, plays an important role in cell signaling and regulates a wide range of biological functions, including the actions of estrogens. Sphingolipids 65-78 sphingosine kinase 1 Homo sapiens 20-24 19074142-4 2009 SphK1 is a lipid kinase that produces the pro-growth, anti-apoptotic sphingosine 1-phosphate, which can induce invasion of glioblastoma cells. sphingosine 1-phosphate 69-92 sphingosine kinase 1 Homo sapiens 0-5 18983913-5 2009 The protective action of TGFbeta from staurosporine-induced apoptosis was strongly reduced when the SphK activity was inhibited by drugs, when SphK1 but not SphK2 was downregulated by specific siRNA and when a SphK1 dominant negative mutant was overexpressed. Staurosporine 38-51 sphingosine kinase 1 Homo sapiens 100-104 18983913-5 2009 The protective action of TGFbeta from staurosporine-induced apoptosis was strongly reduced when the SphK activity was inhibited by drugs, when SphK1 but not SphK2 was downregulated by specific siRNA and when a SphK1 dominant negative mutant was overexpressed. Staurosporine 38-51 sphingosine kinase 1 Homo sapiens 143-148 18983913-5 2009 The protective action of TGFbeta from staurosporine-induced apoptosis was strongly reduced when the SphK activity was inhibited by drugs, when SphK1 but not SphK2 was downregulated by specific siRNA and when a SphK1 dominant negative mutant was overexpressed. Staurosporine 38-51 sphingosine kinase 1 Homo sapiens 210-215 18983913-6 2009 Staurosporine treatment induced down-regulation of both SphK isoforms and TGFbeta rescued SphK1 but not SphK2 expression. Staurosporine 0-13 sphingosine kinase 1 Homo sapiens 56-60 18983913-6 2009 Staurosporine treatment induced down-regulation of both SphK isoforms and TGFbeta rescued SphK1 but not SphK2 expression. Staurosporine 0-13 sphingosine kinase 1 Homo sapiens 90-95 18983913-7 2009 Interestingly, TGFbeta strongly enhanced SphK activity during staurosporine-induced cell death. Staurosporine 62-75 sphingosine kinase 1 Homo sapiens 41-45 19070545-1 2009 Sphingosine-1-phosphate (S1P) is a bioactive lysophospholipid generated by the sphingosine kinase (SK1 or SK2)-catalysed phosphorylation of sphingosine. Lysophospholipids 45-61 sphingosine kinase 1 Homo sapiens 99-102 19070545-1 2009 Sphingosine-1-phosphate (S1P) is a bioactive lysophospholipid generated by the sphingosine kinase (SK1 or SK2)-catalysed phosphorylation of sphingosine. Sphingosine 79-90 sphingosine kinase 1 Homo sapiens 99-102 18852266-3 2008 We have previously identified extracellular signal-regulated kinases 1 and 2 (ERK1/2) as the kinases responsible for the phosphorylation of human SK1 at Ser(225), but the corresponding phosphatase targeting this phosphorylation has remained undefined. Serine 153-156 sphingosine kinase 1 Homo sapiens 146-149 19147534-2 2009 We have previously shown that the bioactive lipid sphingosine-1-phosphate (S1P) stimulates in vitro invasiveness of GBM cells and that high expression levels of the enzyme that forms S1P, sphingosine kinase-1 (SphK1), correlate with shorter survival time of GBM patients. sphingosine 1-phosphate 50-73 sphingosine kinase 1 Homo sapiens 188-208 19147534-2 2009 We have previously shown that the bioactive lipid sphingosine-1-phosphate (S1P) stimulates in vitro invasiveness of GBM cells and that high expression levels of the enzyme that forms S1P, sphingosine kinase-1 (SphK1), correlate with shorter survival time of GBM patients. sphingosine 1-phosphate 50-73 sphingosine kinase 1 Homo sapiens 210-215 19075223-8 2008 We observed significant induction of sphingosine kinase 1 (SPHK1), which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (S1P), in mature multinucleated osteoclasts as compared with preosteoclasts. Sphingosine 37-48 sphingosine kinase 1 Homo sapiens 59-64 19075223-8 2008 We observed significant induction of sphingosine kinase 1 (SPHK1), which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (S1P), in mature multinucleated osteoclasts as compared with preosteoclasts. sphingosine 1-phosphate 126-149 sphingosine kinase 1 Homo sapiens 37-57 19075223-8 2008 We observed significant induction of sphingosine kinase 1 (SPHK1), which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (S1P), in mature multinucleated osteoclasts as compared with preosteoclasts. sphingosine 1-phosphate 126-149 sphingosine kinase 1 Homo sapiens 59-64 18852266-4 2008 Here, we provide data to support a role for protein phosphatase 2A (PP2A) in the deactivation of SK1 through dephosphorylation of phospho-Ser(225). Serine 138-141 sphingosine kinase 1 Homo sapiens 97-100 18852266-6 2008 Coexpression of PP2Ac with SK1 resulted in reduced Ser(225) phosphorylation of SK1 in human embryonic kidney (HEK293) cells. Serine 51-54 sphingosine kinase 1 Homo sapiens 27-30 18852266-6 2008 Coexpression of PP2Ac with SK1 resulted in reduced Ser(225) phosphorylation of SK1 in human embryonic kidney (HEK293) cells. Serine 51-54 sphingosine kinase 1 Homo sapiens 79-82 18852266-7 2008 In vitro phosphatase assays showed that PP2Ac dephosphorylated both recombinant SK1 and a phosphopeptide based on the phospho-Ser(225) region of SK1. Serine 126-129 sphingosine kinase 1 Homo sapiens 145-148 18924207-4 2008 The importance of sphingolipids as potent biological mediators of both intracellular and extracellular responses is being increasingly recognized and accepted; it is now appreciated that activation of mast cells, via the high-affinity IgE-receptor (FcepsilonRI) leads to the activation of sphingosine kinases (SphK), resulting in increased formation of sphingosine-1-phosphate. Sphingolipids 18-31 sphingosine kinase 1 Homo sapiens 310-314 18058224-0 2008 Microarray analysis of altered sphingolipid metabolism reveals prognostic significance of sphingosine kinase 1 in breast cancer. Sphingolipids 31-43 sphingosine kinase 1 Homo sapiens 90-110 18058224-11 2008 The correlation of SPHK1 with a poor prognosis as well as its high expression among ER negative tumors are in line with the antiapoptotic and proliferative properties of its product sphingosine-1-phosphate. sphingosine 1-phosphate 182-205 sphingosine kinase 1 Homo sapiens 19-24 18980995-6 2008 Immunohistochemical analysis showed that 100 of 243 (41.2%) paraffin-embedded archival astrocytoma biopsies exhibited high expression of SPHK1. Paraffin 60-68 sphingosine kinase 1 Homo sapiens 137-142 18924207-5 2008 Furthermore, FcepsilonRI activates SphK-dependent calcium mobilization in mast cells, leading to degranulation, cytokine, and eicosanoid production, and chemotaxis. Calcium 50-57 sphingosine kinase 1 Homo sapiens 35-39 18924207-5 2008 Furthermore, FcepsilonRI activates SphK-dependent calcium mobilization in mast cells, leading to degranulation, cytokine, and eicosanoid production, and chemotaxis. Eicosanoids 126-136 sphingosine kinase 1 Homo sapiens 35-39 18568950-1 2008 Sphingolipids, in particular sphingosine kinase (SphK) and its product sphingosine-1-phosphate (S1P), are now recognized to play an important role in regulating many critical processes in endothelial cells. Sphingolipids 0-13 sphingosine kinase 1 Homo sapiens 29-47 18922940-1 2008 Here, we provide the first evidence that sphingosine kinase 1 (SphK1), an oncogenic lipid kinase balancing the intracellular level of key signaling sphingolipids, modulates the transcription factor hypoxia inducible factor 1alpha (HIF-1alpha), master regulator of hypoxia. Sphingolipids 148-161 sphingosine kinase 1 Homo sapiens 41-61 18922940-1 2008 Here, we provide the first evidence that sphingosine kinase 1 (SphK1), an oncogenic lipid kinase balancing the intracellular level of key signaling sphingolipids, modulates the transcription factor hypoxia inducible factor 1alpha (HIF-1alpha), master regulator of hypoxia. Sphingolipids 148-161 sphingosine kinase 1 Homo sapiens 63-68 18922940-2 2008 SphK1 activity is stimulated under low oxygen conditions and regulated by reactive oxygen species. Oxygen 39-45 sphingosine kinase 1 Homo sapiens 0-5 18922940-2 2008 SphK1 activity is stimulated under low oxygen conditions and regulated by reactive oxygen species. Reactive Oxygen Species 74-97 sphingosine kinase 1 Homo sapiens 0-5 18485923-4 2008 The relative levels of these three interconvertible sphingolipid metabolites, and thus cell fate, are strongly influenced by the activity of sphingosine kinases, of which there are two isoforms, designated SphK1 and SphK2, the enzymes that phosphorylate sphingosine to produce S1P. Sphingolipids 52-64 sphingosine kinase 1 Homo sapiens 206-211 18485923-4 2008 The relative levels of these three interconvertible sphingolipid metabolites, and thus cell fate, are strongly influenced by the activity of sphingosine kinases, of which there are two isoforms, designated SphK1 and SphK2, the enzymes that phosphorylate sphingosine to produce S1P. Sphingosine 141-152 sphingosine kinase 1 Homo sapiens 206-211 18385762-4 2008 K6PC-5, a hydrophobic compound chemically named N-(1,3-dihydroxyisopropyl)-2-hexyl-3-oxo-decanamide, activated SphK (obtained from C57BL/6 murine blood and F9-12 cell lysates) in a dose-dependent manner. N-(1,3-dihydroxyisopropyl)-2-hexyl-3-oxodecanamide 48-99 sphingosine kinase 1 Homo sapiens 111-115 18644866-1 2008 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to produce the potent lipid mediator sphingosine-1-phosphate (S1P), which plays a critical role in cell motility via its cell surface receptors. Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 0-20 18644866-1 2008 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to produce the potent lipid mediator sphingosine-1-phosphate (S1P), which plays a critical role in cell motility via its cell surface receptors. Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 22-27 18644866-1 2008 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to produce the potent lipid mediator sphingosine-1-phosphate (S1P), which plays a critical role in cell motility via its cell surface receptors. sphingosine 1-phosphate 111-134 sphingosine kinase 1 Homo sapiens 0-20 18644866-1 2008 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to produce the potent lipid mediator sphingosine-1-phosphate (S1P), which plays a critical role in cell motility via its cell surface receptors. sphingosine 1-phosphate 111-134 sphingosine kinase 1 Homo sapiens 22-27 18701480-4 2008 Here, we report that LPA markedly enhanced SphK1 mRNA and protein in gastric cancer MKN1 cells but had no effect on SphK2. lysophosphatidic acid 21-24 sphingosine kinase 1 Homo sapiens 43-48 18701480-6 2008 An LPA(1) receptor antagonist or down-regulation of its expression prevented SphK1 and S1P(3) receptor up-regulation by LPA. lysophosphatidic acid 3-6 sphingosine kinase 1 Homo sapiens 77-82 18701480-7 2008 LPA transactivated the epidermal growth factor receptor (EGFR) in these cells, and the EGFR inhibitor AG1478 attenuated the increased SphK1 and S1P(3) expression induced by LPA. RTKI cpd 102-108 sphingosine kinase 1 Homo sapiens 134-139 18701480-8 2008 Moreover, down-regulation of SphK1 attenuated LPA-stimulated migration and invasion of MNK1 cells yet had no effect on expression of neovascularizing factors, such as interleukin (IL)-8, IL-6, urokinase-type plasminogen activator (uPA), or uPA receptor induced by LPA. lysophosphatidic acid 46-49 sphingosine kinase 1 Homo sapiens 29-34 18701480-8 2008 Moreover, down-regulation of SphK1 attenuated LPA-stimulated migration and invasion of MNK1 cells yet had no effect on expression of neovascularizing factors, such as interleukin (IL)-8, IL-6, urokinase-type plasminogen activator (uPA), or uPA receptor induced by LPA. lysophosphatidic acid 264-267 sphingosine kinase 1 Homo sapiens 29-34 18502815-7 2008 The S1P-induced calcium flux and AA release was associated with sphingosine kinase 1 (Sphk1) expression and activity. Calcium 16-23 sphingosine kinase 1 Homo sapiens 86-91 18362204-2 2008 Here we report that treatment of Jurkat and U937 leukemia cells with the pan-sphingosine kinase (SphK) inhibitor N,N-dimethylsphingosine to block S1P formation surprisingly caused a large increase in expression of SphK1 concomitant with induction of apoptosis. N,N-dimethylsphingosine 113-136 sphingosine kinase 1 Homo sapiens 73-95 18362204-2 2008 Here we report that treatment of Jurkat and U937 leukemia cells with the pan-sphingosine kinase (SphK) inhibitor N,N-dimethylsphingosine to block S1P formation surprisingly caused a large increase in expression of SphK1 concomitant with induction of apoptosis. N,N-dimethylsphingosine 113-136 sphingosine kinase 1 Homo sapiens 97-101 18362204-2 2008 Here we report that treatment of Jurkat and U937 leukemia cells with the pan-sphingosine kinase (SphK) inhibitor N,N-dimethylsphingosine to block S1P formation surprisingly caused a large increase in expression of SphK1 concomitant with induction of apoptosis. N,N-dimethylsphingosine 113-136 sphingosine kinase 1 Homo sapiens 214-219 18362204-3 2008 Another SphK inhibitor, D,L-threo-dihydrosphingosine, also induced apoptosis and produced dramatic increases in SphK1 expression. d,l-threo-dihydrosphingosine 24-52 sphingosine kinase 1 Homo sapiens 8-12 18362204-3 2008 Another SphK inhibitor, D,L-threo-dihydrosphingosine, also induced apoptosis and produced dramatic increases in SphK1 expression. d,l-threo-dihydrosphingosine 24-52 sphingosine kinase 1 Homo sapiens 112-117 18362204-5 2008 The chemotherapeutic drug doxorubicin, a potent inducer of apoptosis in these cells, also stimulated SphK1 expression and activity and promoted S1P secretion. Doxorubicin 26-37 sphingosine kinase 1 Homo sapiens 101-106 18362204-6 2008 The caspase inhibitor ZVAD reduced not only doxorubicin-induced lethality but also the increased expression of SphK1 and secretion of S1P. benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone 22-26 sphingosine kinase 1 Homo sapiens 111-116 18420384-11 2008 Both dimethylsphingosine and dihydroxysphingosine, SK inhibitors, and the transfection of SK1-siRNA blocked the K6PC-5-induced increases in [Ca(2+)](i), an effect independent of the classical PLC/IP(3)-mediated pathway. K6PC-5 112-118 sphingosine kinase 1 Homo sapiens 90-93 18602364-3 2008 Sphingosine kinase (SPHK) catalyzes the formation of sphingosine 1-phosphate (S1P), which acts as a second messenger to function as an anti-apoptotic factor and proliferation stimulator of immune cells. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 0-18 18602364-3 2008 Sphingosine kinase (SPHK) catalyzes the formation of sphingosine 1-phosphate (S1P), which acts as a second messenger to function as an anti-apoptotic factor and proliferation stimulator of immune cells. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 20-24 18602364-3 2008 Sphingosine kinase (SPHK) catalyzes the formation of sphingosine 1-phosphate (S1P), which acts as a second messenger to function as an anti-apoptotic factor and proliferation stimulator of immune cells. sphingosine 1-phosphate 78-81 sphingosine kinase 1 Homo sapiens 0-18 18602364-3 2008 Sphingosine kinase (SPHK) catalyzes the formation of sphingosine 1-phosphate (S1P), which acts as a second messenger to function as an anti-apoptotic factor and proliferation stimulator of immune cells. sphingosine 1-phosphate 78-81 sphingosine kinase 1 Homo sapiens 20-24 18511810-1 2008 The potent bioactive sphingolipid mediator, sphingosine-1-phosphate (S1P), is produced by 2 sphingosine kinase isoenzymes, SphK1 and SphK2. Sphingolipids 21-33 sphingosine kinase 1 Homo sapiens 123-128 18511810-1 2008 The potent bioactive sphingolipid mediator, sphingosine-1-phosphate (S1P), is produced by 2 sphingosine kinase isoenzymes, SphK1 and SphK2. sphingosine 1-phosphate 44-67 sphingosine kinase 1 Homo sapiens 123-128 18511810-1 2008 The potent bioactive sphingolipid mediator, sphingosine-1-phosphate (S1P), is produced by 2 sphingosine kinase isoenzymes, SphK1 and SphK2. sphingosine 1-phosphate 69-72 sphingosine kinase 1 Homo sapiens 123-128 18511810-3 2008 A screen of sphingosine analogs identified (2R,3S,4E)-N-methyl-5-(4"-pentylphenyl)-2-aminopent-4-ene-1,3-diol, designated SK1-I (BML-258), as a potent, water-soluble, isoenzyme-specific inhibitor of SphK1. Sphingosine 12-23 sphingosine kinase 1 Homo sapiens 122-125 18511810-3 2008 A screen of sphingosine analogs identified (2R,3S,4E)-N-methyl-5-(4"-pentylphenyl)-2-aminopent-4-ene-1,3-diol, designated SK1-I (BML-258), as a potent, water-soluble, isoenzyme-specific inhibitor of SphK1. (2r,3s,4e)-n-methyl-5-(4"-pentylphenyl)-2-aminopent-4-ene-1,3-diol 43-109 sphingosine kinase 1 Homo sapiens 122-125 18511810-3 2008 A screen of sphingosine analogs identified (2R,3S,4E)-N-methyl-5-(4"-pentylphenyl)-2-aminopent-4-ene-1,3-diol, designated SK1-I (BML-258), as a potent, water-soluble, isoenzyme-specific inhibitor of SphK1. (2r,3s,4e)-n-methyl-5-(4"-pentylphenyl)-2-aminopent-4-ene-1,3-diol 43-109 sphingosine kinase 1 Homo sapiens 199-204 18511810-3 2008 A screen of sphingosine analogs identified (2R,3S,4E)-N-methyl-5-(4"-pentylphenyl)-2-aminopent-4-ene-1,3-diol, designated SK1-I (BML-258), as a potent, water-soluble, isoenzyme-specific inhibitor of SphK1. N-methyl-5-(4'-pentylphenyl)-2-aminopent-4-ene-1,3-diol 129-136 sphingosine kinase 1 Homo sapiens 122-125 18511810-3 2008 A screen of sphingosine analogs identified (2R,3S,4E)-N-methyl-5-(4"-pentylphenyl)-2-aminopent-4-ene-1,3-diol, designated SK1-I (BML-258), as a potent, water-soluble, isoenzyme-specific inhibitor of SphK1. Water 152-157 sphingosine kinase 1 Homo sapiens 122-125 18511810-7 2008 SK1-I not only decreased S1P levels but concomitantly increased levels of its proapoptotic precursor ceramide. Ceramides 101-109 sphingosine kinase 1 Homo sapiens 0-3 18502815-7 2008 The S1P-induced calcium flux and AA release was associated with sphingosine kinase 1 (Sphk1) expression and activity. Calcium 16-23 sphingosine kinase 1 Homo sapiens 64-84 18687064-4 2008 Using the inducible expression of sphingosine kinase 1 and 2 in HEK293 cells as model systems, we found this ARE approach to be remarkably successful in ablating expression of these proteins in the absence of doxycycline through decreasing stability of their mRNAs. Doxycycline 209-220 sphingosine kinase 1 Homo sapiens 34-60 18718048-3 2008 The results indicated that after being cultured with 2.5 micromol/L imatinib mesylate for 0.5, 2, 6, 24 and 48 hours, the intensions of inhibiting SphK-1 activity were 0.007%, 38.9%, 34.6%, 28.1% and 76.1% resepectively. Imatinib Mesylate 68-85 sphingosine kinase 1 Homo sapiens 147-153 18718048-4 2008 SphK-1 activity in CML cells also was reduced by 2.5 micromol/L imatinib mesylate (16.8% - 41.9% decrease). Imatinib Mesylate 64-81 sphingosine kinase 1 Homo sapiens 0-6 18644996-4 2008 Importantly, pharmacologic SphK1 inhibition with the B-5354c compound sensitizes LNCaP and PC-3 cells to docetaxel and camptothecin, respectively. Docetaxel 105-114 sphingosine kinase 1 Homo sapiens 27-32 18644996-4 2008 Importantly, pharmacologic SphK1 inhibition with the B-5354c compound sensitizes LNCaP and PC-3 cells to docetaxel and camptothecin, respectively. Camptothecin 119-131 sphingosine kinase 1 Homo sapiens 27-32 18192241-0 2008 Activation of sphingosine kinase-1 mediates induction of endothelial cell proliferation and angiogenesis by epoxyeicosatrienoic acids. epoxyeicosatrienoic acids 108-133 sphingosine kinase 1 Homo sapiens 14-34 18568950-1 2008 Sphingolipids, in particular sphingosine kinase (SphK) and its product sphingosine-1-phosphate (S1P), are now recognized to play an important role in regulating many critical processes in endothelial cells. Sphingolipids 0-13 sphingosine kinase 1 Homo sapiens 49-53 18568950-1 2008 Sphingolipids, in particular sphingosine kinase (SphK) and its product sphingosine-1-phosphate (S1P), are now recognized to play an important role in regulating many critical processes in endothelial cells. sphingosine 1-phosphate 71-94 sphingosine kinase 1 Homo sapiens 29-47 18568950-1 2008 Sphingolipids, in particular sphingosine kinase (SphK) and its product sphingosine-1-phosphate (S1P), are now recognized to play an important role in regulating many critical processes in endothelial cells. sphingosine 1-phosphate 71-94 sphingosine kinase 1 Homo sapiens 49-53 17599053-5 2007 Inhibition of SPK1 by adenovirus-mediated transfer of small interfering RNA or N,N-dimethylsphingosine reduced expression of Mcl-1 in CML cells. N,N-dimethylsphingosine 79-102 sphingosine kinase 1 Homo sapiens 14-18 18192241-10 2008 Furthermore, knockdown of SK1 expression by specific siRNA also inhibited 11,12-EET-induced EC proliferation and migration, whereas SK2 siRNA knockdown was without effect. 11,12-epoxy-5,8,14-eicosatrienoic acid 74-83 sphingosine kinase 1 Homo sapiens 26-29 18192241-11 2008 CONCLUSION: These results suggest that SK1 is an important mediator of the 11,12-EET-induced angiogenic effects in human ECs. 11,12-epoxy-5,8,14-eicosatrienoic acid 75-84 sphingosine kinase 1 Homo sapiens 39-42 17913601-4 2008 Ceramide is formed from SM by the action of sphingomyelinases (SMase), however, ceramide can be very rapidly hydrolysed, by ceramidases to yield sphingosine, and sphingosine can be phosphorylated by sphingosine kinase (SphK) to yield S1P. Ceramides 0-8 sphingosine kinase 1 Homo sapiens 199-217 17913601-4 2008 Ceramide is formed from SM by the action of sphingomyelinases (SMase), however, ceramide can be very rapidly hydrolysed, by ceramidases to yield sphingosine, and sphingosine can be phosphorylated by sphingosine kinase (SphK) to yield S1P. Ceramides 0-8 sphingosine kinase 1 Homo sapiens 219-223 17913601-4 2008 Ceramide is formed from SM by the action of sphingomyelinases (SMase), however, ceramide can be very rapidly hydrolysed, by ceramidases to yield sphingosine, and sphingosine can be phosphorylated by sphingosine kinase (SphK) to yield S1P. Ceramides 80-88 sphingosine kinase 1 Homo sapiens 199-217 17913601-4 2008 Ceramide is formed from SM by the action of sphingomyelinases (SMase), however, ceramide can be very rapidly hydrolysed, by ceramidases to yield sphingosine, and sphingosine can be phosphorylated by sphingosine kinase (SphK) to yield S1P. Ceramides 80-88 sphingosine kinase 1 Homo sapiens 219-223 17913601-4 2008 Ceramide is formed from SM by the action of sphingomyelinases (SMase), however, ceramide can be very rapidly hydrolysed, by ceramidases to yield sphingosine, and sphingosine can be phosphorylated by sphingosine kinase (SphK) to yield S1P. Sphingosine 162-173 sphingosine kinase 1 Homo sapiens 199-217 17913601-4 2008 Ceramide is formed from SM by the action of sphingomyelinases (SMase), however, ceramide can be very rapidly hydrolysed, by ceramidases to yield sphingosine, and sphingosine can be phosphorylated by sphingosine kinase (SphK) to yield S1P. Sphingosine 162-173 sphingosine kinase 1 Homo sapiens 219-223 17654519-1 2008 Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes the formation of sphingosine 1-phosphate (S1P), an important lipid mediator, which regulates fundamental biological processes. sphingosine 1-phosphate 86-109 sphingosine kinase 1 Homo sapiens 0-18 17654519-1 2008 Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes the formation of sphingosine 1-phosphate (S1P), an important lipid mediator, which regulates fundamental biological processes. sphingosine 1-phosphate 86-109 sphingosine kinase 1 Homo sapiens 20-24 18401414-0 2008 Sphingosine kinase-1 is a downstream regulator of imatinib-induced apoptosis in chronic myeloid leukemia cells. Imatinib Mesylate 50-58 sphingosine kinase 1 Homo sapiens 0-20 18401414-1 2008 We examined the involvement of sphingosine kinase-1 (SphK1), which governs the ceramide/sphingosine-1-phosphate balance, in susceptibility to imatinib of either sensitive or resistant chronic myeloid leukemia cells. Ceramides 79-87 sphingosine kinase 1 Homo sapiens 31-51 18401414-1 2008 We examined the involvement of sphingosine kinase-1 (SphK1), which governs the ceramide/sphingosine-1-phosphate balance, in susceptibility to imatinib of either sensitive or resistant chronic myeloid leukemia cells. Ceramides 79-87 sphingosine kinase 1 Homo sapiens 53-58 18401414-1 2008 We examined the involvement of sphingosine kinase-1 (SphK1), which governs the ceramide/sphingosine-1-phosphate balance, in susceptibility to imatinib of either sensitive or resistant chronic myeloid leukemia cells. sphingosine 1-phosphate 88-111 sphingosine kinase 1 Homo sapiens 53-58 18401414-1 2008 We examined the involvement of sphingosine kinase-1 (SphK1), which governs the ceramide/sphingosine-1-phosphate balance, in susceptibility to imatinib of either sensitive or resistant chronic myeloid leukemia cells. Imatinib Mesylate 142-150 sphingosine kinase 1 Homo sapiens 31-51 18401414-1 2008 We examined the involvement of sphingosine kinase-1 (SphK1), which governs the ceramide/sphingosine-1-phosphate balance, in susceptibility to imatinib of either sensitive or resistant chronic myeloid leukemia cells. Imatinib Mesylate 142-150 sphingosine kinase 1 Homo sapiens 53-58 18401414-2 2008 Imatinib-sensitive LAMA84-s displayed marked SphK1 inhibition coupled with increased content of ceramide and decreased pro-survival sphingosine-1-phosphate. Imatinib Mesylate 0-8 sphingosine kinase 1 Homo sapiens 45-50 18401414-4 2008 Overcoming imatinib resistance in LAMA84-r with farnesyltransferase or MEK/ERK inhibitors as well as with cytosine arabinoside led to SphK1 inhibition. Imatinib Mesylate 11-19 sphingosine kinase 1 Homo sapiens 134-139 18401414-4 2008 Overcoming imatinib resistance in LAMA84-r with farnesyltransferase or MEK/ERK inhibitors as well as with cytosine arabinoside led to SphK1 inhibition. Cytarabine 106-126 sphingosine kinase 1 Homo sapiens 134-139 18401414-5 2008 Overexpression of SphK1 in LAMA84-s cells impaired apoptosis and inhibited the effects of imatinib on caspase-3 activation, cytochrome c and Smac release from mitochondria through modulation of Bim, Bcl-xL and Mcl-1 expression. Imatinib Mesylate 90-98 sphingosine kinase 1 Homo sapiens 18-23 18401414-6 2008 Pharmacological inhibition of SphK1 with F-12509a or its silencing by siRNA induced apoptosis of both imatinib-sensitive and -resistant cells, suggesting that SphK1 inhibition was critical for apoptosis signaling. Imatinib Mesylate 102-110 sphingosine kinase 1 Homo sapiens 30-35 18401414-8 2008 These results uncover the involvement of SphK1 in regulating imatinib-induced apoptosis and establish that SphK1 is a downstream effector of the Bcr-Abl/Ras/ERK pathway inhibited by imatinib but upstream regulator of Bcl-2 family members. Imatinib Mesylate 61-69 sphingosine kinase 1 Homo sapiens 41-46 18401414-8 2008 These results uncover the involvement of SphK1 in regulating imatinib-induced apoptosis and establish that SphK1 is a downstream effector of the Bcr-Abl/Ras/ERK pathway inhibited by imatinib but upstream regulator of Bcl-2 family members. Imatinib Mesylate 182-190 sphingosine kinase 1 Homo sapiens 107-112 18283525-0 2008 Implications of sphingosine kinase 1 expression level for the cellular sphingolipid rheostat: relevance as a marker for daunorubicin sensitivity of leukemia cells. Sphingolipids 71-83 sphingosine kinase 1 Homo sapiens 16-36 18283525-0 2008 Implications of sphingosine kinase 1 expression level for the cellular sphingolipid rheostat: relevance as a marker for daunorubicin sensitivity of leukemia cells. Daunorubicin 120-132 sphingosine kinase 1 Homo sapiens 16-36 18283525-2 2008 This alteration is supposed to change the cellular sphingolipid metabolites; however, positive correlations were observed between daunorubicin (DA)-IC50 and the SPHK1 message but not between DA-IC50 and NSMase2 messages, when 16 different leukemia cell lines were used to analyze the relationship between gene expressions and chemosensitivity against DA. Daunorubicin 130-142 sphingosine kinase 1 Homo sapiens 161-166 18283525-2 2008 This alteration is supposed to change the cellular sphingolipid metabolites; however, positive correlations were observed between daunorubicin (DA)-IC50 and the SPHK1 message but not between DA-IC50 and NSMase2 messages, when 16 different leukemia cell lines were used to analyze the relationship between gene expressions and chemosensitivity against DA. Daunorubicin 144-146 sphingosine kinase 1 Homo sapiens 161-166 18283525-6 2008 A SPHK inhibitor recovered the DA sensitivity of DA-resistant cells. Daunorubicin 31-33 sphingosine kinase 1 Homo sapiens 2-6 18166496-1 2008 Sphingosine kinase 1 (SK1) and its product sphingosine-1-phosphate (S1P) have been implicated in the regulation of many cellular processes including growth regulation, protection from apoptosis, stimulation of angiogenesis, and most recently as mediators of the TNF-alpha inflammatory response. sphingosine 1-phosphate 43-66 sphingosine kinase 1 Homo sapiens 0-20 18166496-1 2008 Sphingosine kinase 1 (SK1) and its product sphingosine-1-phosphate (S1P) have been implicated in the regulation of many cellular processes including growth regulation, protection from apoptosis, stimulation of angiogenesis, and most recently as mediators of the TNF-alpha inflammatory response. sphingosine 1-phosphate 43-66 sphingosine kinase 1 Homo sapiens 22-25 18055454-8 2008 Interestingly, knockdown of HIF-2alpha by small interfering RNA abolished the induction of SK1 and the production of extracellular S1P after CoCl(2) treatment, whereas HIF-1alpha small interfering RNA resulted in an increase of HIF-2alpha and of SK1 protein levels. cobaltous chloride 141-148 sphingosine kinase 1 Homo sapiens 91-94 17855624-6 2008 Similarly, inhibition of c-Src activity by PP2 blocked both EGF-induced translocation of SphK1 and PKCdelta to the plasma membrane and up-regulation of PAI-1 expression. pp2 43-46 sphingosine kinase 1 Homo sapiens 89-94 17599053-7 2007 SPK1 silencing enhances the STI571-induced apoptosis of CML cell lines. Imatinib Mesylate 28-34 sphingosine kinase 1 Homo sapiens 0-4 17898040-7 2007 SK activity as well as SK1 mRNA expression increased in both cultured human proximal tubule cells (HK-2) and mouse kidneys after exposure to isoflurane. Isoflurane 141-151 sphingosine kinase 1 Homo sapiens 23-26 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 141-159 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 161-165 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 179-184 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. Sphingosine 80-91 sphingosine kinase 1 Homo sapiens 141-159 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. Sphingosine 80-91 sphingosine kinase 1 Homo sapiens 161-165 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. Sphingosine 80-91 sphingosine kinase 1 Homo sapiens 179-184 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. Sphingolipids 109-122 sphingosine kinase 1 Homo sapiens 141-159 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. Sphingolipids 109-122 sphingosine kinase 1 Homo sapiens 161-165 17974990-1 2007 Sphingosine-1-phosphate is a potent lipid mediator formed by phosphorylation of sphingosine, a metabolite of sphingolipids, catalyzed by two sphingosine kinase (SphK) isoenzymes, SphK1 and SphK2. Sphingolipids 109-122 sphingosine kinase 1 Homo sapiens 179-184 17947634-1 2007 Sphingosine kinase (Sphk) phosphorylates sphingosine into sphingosine-1-phosphate (S1P), but its recently identified isoform Sphk2 has been suggested to have distinct subcellular localization and substrate specificity. Sphingosine 41-52 sphingosine kinase 1 Homo sapiens 0-18 17947634-1 2007 Sphingosine kinase (Sphk) phosphorylates sphingosine into sphingosine-1-phosphate (S1P), but its recently identified isoform Sphk2 has been suggested to have distinct subcellular localization and substrate specificity. Sphingosine 41-52 sphingosine kinase 1 Homo sapiens 20-24 17947634-1 2007 Sphingosine kinase (Sphk) phosphorylates sphingosine into sphingosine-1-phosphate (S1P), but its recently identified isoform Sphk2 has been suggested to have distinct subcellular localization and substrate specificity. sphingosine 1-phosphate 58-81 sphingosine kinase 1 Homo sapiens 0-18 17947634-1 2007 Sphingosine kinase (Sphk) phosphorylates sphingosine into sphingosine-1-phosphate (S1P), but its recently identified isoform Sphk2 has been suggested to have distinct subcellular localization and substrate specificity. sphingosine 1-phosphate 58-81 sphingosine kinase 1 Homo sapiens 20-24 17635916-1 2007 Sphingosine kinase (SPHK) is a key enzyme producing important messenger sphingosine 1-phosphate and is implicated in cell proliferation and suppression of apoptosis. sphingosine 1-phosphate 72-95 sphingosine kinase 1 Homo sapiens 0-18 17623044-4 2007 SPHK activity, measured by the conversion of [(3)H]sphingosine to [(3)H]S1P, is highest in cerebellum, followed by cortex and brainstem. [(3)h]sphingosine 45-62 sphingosine kinase 1 Homo sapiens 0-4 17623044-4 2007 SPHK activity, measured by the conversion of [(3)H]sphingosine to [(3)H]S1P, is highest in cerebellum, followed by cortex and brainstem. [(3)h]s1p 66-75 sphingosine kinase 1 Homo sapiens 0-4 17635916-1 2007 Sphingosine kinase (SPHK) is a key enzyme producing important messenger sphingosine 1-phosphate and is implicated in cell proliferation and suppression of apoptosis. sphingosine 1-phosphate 72-95 sphingosine kinase 1 Homo sapiens 20-24 17643421-0 2007 Sphingosine kinase 1 is a critical component of the copper-dependent FGF1 export pathway. Copper 52-58 sphingosine kinase 1 Homo sapiens 0-20 17643421-1 2007 Sphingosine kinase 1 catalyzes the formation of sphingosine-1-phosphate, a lipid mediator involved in the regulation of angiogenesis. sphingosine 1-phosphate 48-71 sphingosine kinase 1 Homo sapiens 0-20 17786207-7 2007 METHODOLOGY/PRINCIPAL FINDINGS: We found that functional inhibition of endogenous RARalpha in breast cancer cells by using either RARalpha specific antagonists or a dominant negative RARalpha mutant hampers on one hand the RA-induced upregulation of neutral sphingomyelinase (nSMase)-mediated CER synthesis, and on the other hand the RA-induced downregulation of sphingosine kinase 1, SK1, pivotal for S1P synthesis. Tretinoin 82-84 sphingosine kinase 1 Homo sapiens 363-383 17786207-7 2007 METHODOLOGY/PRINCIPAL FINDINGS: We found that functional inhibition of endogenous RARalpha in breast cancer cells by using either RARalpha specific antagonists or a dominant negative RARalpha mutant hampers on one hand the RA-induced upregulation of neutral sphingomyelinase (nSMase)-mediated CER synthesis, and on the other hand the RA-induced downregulation of sphingosine kinase 1, SK1, pivotal for S1P synthesis. Tretinoin 82-84 sphingosine kinase 1 Homo sapiens 385-388 17786207-9 2007 By combining genetic, pharmacological and biochemical approaches, we mechanistically demonstrated that RA-induced growth is, at least in part, due to non-RAR-mediated activation of the SK1-S1P signaling. Tretinoin 103-105 sphingosine kinase 1 Homo sapiens 185-188 17686057-6 2007 It was further demonstrated that IL-6-induced activation of SPHK inhibited dexamethasone-induced apoptosis of MM cells. Dexamethasone 75-88 sphingosine kinase 1 Homo sapiens 60-64 17643421-5 2007 We propose that sphingosine kinase 1 is a component of the copper-dependent FGF1 release pathway. Copper 59-65 sphingosine kinase 1 Homo sapiens 16-36 17504945-0 2007 Suppression of osteoclastogenesis by N,N-dimethyl-D-erythro-sphingosine: a sphingosine kinase inhibition-independent action. N,N-dimethylsphingosine 37-71 sphingosine kinase 1 Homo sapiens 75-93 17504945-1 2007 N,N-Dimethyl-D-erythro-sphingosine (DMS) competitively inhibits sphingosine kinase (SPHK) and has been widely used to assess the role of SPHK during cellular events, including motility, proliferation, and differentiation. N,N-dimethylsphingosine 0-34 sphingosine kinase 1 Homo sapiens 64-82 17504945-1 2007 N,N-Dimethyl-D-erythro-sphingosine (DMS) competitively inhibits sphingosine kinase (SPHK) and has been widely used to assess the role of SPHK during cellular events, including motility, proliferation, and differentiation. N,N-dimethylsphingosine 0-34 sphingosine kinase 1 Homo sapiens 84-88 17504945-1 2007 N,N-Dimethyl-D-erythro-sphingosine (DMS) competitively inhibits sphingosine kinase (SPHK) and has been widely used to assess the role of SPHK during cellular events, including motility, proliferation, and differentiation. N,N-dimethylsphingosine 0-34 sphingosine kinase 1 Homo sapiens 137-141 17504945-1 2007 N,N-Dimethyl-D-erythro-sphingosine (DMS) competitively inhibits sphingosine kinase (SPHK) and has been widely used to assess the role of SPHK during cellular events, including motility, proliferation, and differentiation. N,N-dimethylsphingosine 36-39 sphingosine kinase 1 Homo sapiens 64-82 17504945-1 2007 N,N-Dimethyl-D-erythro-sphingosine (DMS) competitively inhibits sphingosine kinase (SPHK) and has been widely used to assess the role of SPHK during cellular events, including motility, proliferation, and differentiation. N,N-dimethylsphingosine 36-39 sphingosine kinase 1 Homo sapiens 84-88 17504945-1 2007 N,N-Dimethyl-D-erythro-sphingosine (DMS) competitively inhibits sphingosine kinase (SPHK) and has been widely used to assess the role of SPHK during cellular events, including motility, proliferation, and differentiation. N,N-dimethylsphingosine 36-39 sphingosine kinase 1 Homo sapiens 137-141 17522181-1 2007 We examined the role of sphingosine kinase-1 (SphK1), a critical regulator of the ceramide/sphingosine 1-phosphate (S1P) biostat, in the regulation of death and survival of SH-SY5Y neuroblastoma cells in response to amyloid beta (Abeta) peptide (25-35). Ceramides 82-90 sphingosine kinase 1 Homo sapiens 24-44 17699106-0 2007 (Dihydro)ceramide synthase 1 regulated sensitivity to cisplatin is associated with the activation of p38 mitogen-activated protein kinase and is abrogated by sphingosine kinase 1. Cisplatin 54-63 sphingosine kinase 1 Homo sapiens 158-178 17699106-3 2007 Here, we show that the members of the sphingosine kinase (SphK1 and SphK2) and dihydroceramide synthase (LASS1/CerS1, LASS4/CerS4, and LASS5/CerS5) enzyme families each have a unique role in regulating sensitivity to cisplatin and other drugs. Cisplatin 217-226 sphingosine kinase 1 Homo sapiens 58-63 17699106-4 2007 Thus, expression of SphK1 decreases sensitivity to cisplatin, carboplatin, doxorubicin, and vincristine, whereas expression of SphK2 increases sensitivity. Cisplatin 51-60 sphingosine kinase 1 Homo sapiens 20-25 17699106-4 2007 Thus, expression of SphK1 decreases sensitivity to cisplatin, carboplatin, doxorubicin, and vincristine, whereas expression of SphK2 increases sensitivity. Carboplatin 62-73 sphingosine kinase 1 Homo sapiens 20-25 17699106-4 2007 Thus, expression of SphK1 decreases sensitivity to cisplatin, carboplatin, doxorubicin, and vincristine, whereas expression of SphK2 increases sensitivity. Doxorubicin 75-86 sphingosine kinase 1 Homo sapiens 20-25 17699106-4 2007 Thus, expression of SphK1 decreases sensitivity to cisplatin, carboplatin, doxorubicin, and vincristine, whereas expression of SphK2 increases sensitivity. Vincristine 92-103 sphingosine kinase 1 Homo sapiens 20-25 17699106-9 2007 Supporting the hypothesis that this translocation is mechanistically involved in the response to cisplatin, we showed that expression of SphK1, but not SphK2, abrogates both the increased cisplatin sensitivity in cells stably expressing LASS1/CerS and the translocation of the LASS1/CerS1. Cisplatin 97-106 sphingosine kinase 1 Homo sapiens 137-142 17699106-9 2007 Supporting the hypothesis that this translocation is mechanistically involved in the response to cisplatin, we showed that expression of SphK1, but not SphK2, abrogates both the increased cisplatin sensitivity in cells stably expressing LASS1/CerS and the translocation of the LASS1/CerS1. Cisplatin 188-197 sphingosine kinase 1 Homo sapiens 137-142 17639048-4 2007 A similar biphasic effect on SK-1 activity is seen for 17beta-estradiol (E(2)). Estradiol 55-71 sphingosine kinase 1 Homo sapiens 29-33 17522181-1 2007 We examined the role of sphingosine kinase-1 (SphK1), a critical regulator of the ceramide/sphingosine 1-phosphate (S1P) biostat, in the regulation of death and survival of SH-SY5Y neuroblastoma cells in response to amyloid beta (Abeta) peptide (25-35). Ceramides 82-90 sphingosine kinase 1 Homo sapiens 46-51 17522181-1 2007 We examined the role of sphingosine kinase-1 (SphK1), a critical regulator of the ceramide/sphingosine 1-phosphate (S1P) biostat, in the regulation of death and survival of SH-SY5Y neuroblastoma cells in response to amyloid beta (Abeta) peptide (25-35). sphingosine 1-phosphate 91-114 sphingosine kinase 1 Homo sapiens 24-44 17522181-1 2007 We examined the role of sphingosine kinase-1 (SphK1), a critical regulator of the ceramide/sphingosine 1-phosphate (S1P) biostat, in the regulation of death and survival of SH-SY5Y neuroblastoma cells in response to amyloid beta (Abeta) peptide (25-35). sphingosine 1-phosphate 91-114 sphingosine kinase 1 Homo sapiens 46-51 17522181-3 2007 This mechanism was redox-sensitive; N-acetylcysteine totally abrogated the down-regulation of SphK1 activity and strongly inhibited Abeta-induced cell death. Acetylcysteine 36-52 sphingosine kinase 1 Homo sapiens 94-99 17379599-0 2007 Intracellular generation of sphingosine 1-phosphate in human lung endothelial cells: role of lipid phosphate phosphatase-1 and sphingosine kinase 1. sphingosine 1-phosphate 28-51 sphingosine kinase 1 Homo sapiens 127-147 17325258-4 2007 Compared with normoglycemia, exposure of HASMCs to hyperglycemia but not mannitol significantly increased sphingosine kinase 1 (SK1) activity but not SK2 activity. hasmcs 41-47 sphingosine kinase 1 Homo sapiens 106-126 17325258-4 2007 Compared with normoglycemia, exposure of HASMCs to hyperglycemia but not mannitol significantly increased sphingosine kinase 1 (SK1) activity but not SK2 activity. hasmcs 41-47 sphingosine kinase 1 Homo sapiens 128-131 17325258-6 2007 The mechanism of SK1 activation by high glucose involves plasma membrane translocation. Glucose 40-47 sphingosine kinase 1 Homo sapiens 17-20 17325258-8 2007 Importantly, inhibition of SK1 by either a competitive inhibitor N",N"-dimethylsphingosine or expression of dominant-negative mutant of SK1(G82D) or specific small interference RNA knockdown substantially attenuated hyperglycemia-induced anti-apoptotic effect and anti-apoptotic protein Bcl-2 expression in HASMCs. N,N-dimethylsphingosine 65-90 sphingosine kinase 1 Homo sapiens 27-30 17325258-9 2007 Moreover, SK1-mediated anti-apoptotic effect requires the intracellular effects of sphingosine-1-phosphate. sphingosine 1-phosphate 83-106 sphingosine kinase 1 Homo sapiens 10-13 17325258-10 2007 We conclude that hyperglycemia stimulates SK1 activity via PKC- and oxidative stress-dependent pathways, leading to decreased apoptosis in HASMCs. hasmcs 139-145 sphingosine kinase 1 Homo sapiens 42-45 17482291-4 2007 Wortmannin, SH-5, and rapamycin significantly blocked PDGF-stimulated induction of SphK1 mRNA and protein expression, indicating a regulatory role of the PI3K/AKT/mTOR pathway in SphK1 expression. Wortmannin 0-10 sphingosine kinase 1 Homo sapiens 83-88 17325039-5 2007 Silencing SK1 expression by small interfering RNA as well as SK1 inhibition by dimethylsphingosine resulted in a strong inhibition of depolarization-evoked glutamate secretion. dimethylsphingosine 79-98 sphingosine kinase 1 Homo sapiens 61-64 17325039-5 2007 Silencing SK1 expression by small interfering RNA as well as SK1 inhibition by dimethylsphingosine resulted in a strong inhibition of depolarization-evoked glutamate secretion. Glutamic Acid 156-165 sphingosine kinase 1 Homo sapiens 10-13 17325039-5 2007 Silencing SK1 expression by small interfering RNA as well as SK1 inhibition by dimethylsphingosine resulted in a strong inhibition of depolarization-evoked glutamate secretion. Glutamic Acid 156-165 sphingosine kinase 1 Homo sapiens 61-64 17303574-7 2007 However, expression levels of sphingosine kinase-1 (SK1) and generation of sphingosine 1-phosphate (S1P) were increased significantly in K562/IMA-1 cells, channeling sphingoid bases to the sphingosine kinase pathway. sphingoid bases 166-181 sphingosine kinase 1 Homo sapiens 30-55 17303574-8 2007 The partial inhibition of SK1 expression by small interference RNA modulated S1P levels and increased sensitivity to imatinib-induced apoptosis in resistant cells. Imatinib Mesylate 117-125 sphingosine kinase 1 Homo sapiens 26-29 17303574-9 2007 On the other hand, forced expression of SK1 in K562 cells increased the ratio between total S1P/C18-ceramide levels approximately 6-fold and prevented apoptosis significantly in response to imatinib. Ceramides 100-108 sphingosine kinase 1 Homo sapiens 40-43 17303574-9 2007 On the other hand, forced expression of SK1 in K562 cells increased the ratio between total S1P/C18-ceramide levels approximately 6-fold and prevented apoptosis significantly in response to imatinib. Imatinib Mesylate 190-198 sphingosine kinase 1 Homo sapiens 40-43 17303574-10 2007 Additional data indicated a role for SK1/S1P signaling in the up-regulation of the Bcr-Abl expression at the post-transcriptional level, which suggested a possible mechanism for resistance to imatinib-mediated apoptosis. Imatinib Mesylate 192-200 sphingosine kinase 1 Homo sapiens 37-44 17303574-11 2007 In conclusion, these data suggest a role for endogenous C18-ceramide synthesis mainly via hLASS1 in imatinib-induced apoptosis in sensitive cells, whereas in resistant cells, alterations of the balance between the levels of ceramide and S1P by overexpression of SK1 result in resistance to imatinib-induced apoptosis. C18-CERAMIDE 56-68 sphingosine kinase 1 Homo sapiens 262-265 17303574-11 2007 In conclusion, these data suggest a role for endogenous C18-ceramide synthesis mainly via hLASS1 in imatinib-induced apoptosis in sensitive cells, whereas in resistant cells, alterations of the balance between the levels of ceramide and S1P by overexpression of SK1 result in resistance to imatinib-induced apoptosis. Imatinib Mesylate 100-108 sphingosine kinase 1 Homo sapiens 262-265 17482291-4 2007 Wortmannin, SH-5, and rapamycin significantly blocked PDGF-stimulated induction of SphK1 mRNA and protein expression, indicating a regulatory role of the PI3K/AKT/mTOR pathway in SphK1 expression. Wortmannin 0-10 sphingosine kinase 1 Homo sapiens 179-184 17482291-4 2007 Wortmannin, SH-5, and rapamycin significantly blocked PDGF-stimulated induction of SphK1 mRNA and protein expression, indicating a regulatory role of the PI3K/AKT/mTOR pathway in SphK1 expression. SH-5 12-16 sphingosine kinase 1 Homo sapiens 83-88 17482291-4 2007 Wortmannin, SH-5, and rapamycin significantly blocked PDGF-stimulated induction of SphK1 mRNA and protein expression, indicating a regulatory role of the PI3K/AKT/mTOR pathway in SphK1 expression. SH-5 12-16 sphingosine kinase 1 Homo sapiens 179-184 17482291-4 2007 Wortmannin, SH-5, and rapamycin significantly blocked PDGF-stimulated induction of SphK1 mRNA and protein expression, indicating a regulatory role of the PI3K/AKT/mTOR pathway in SphK1 expression. Sirolimus 22-31 sphingosine kinase 1 Homo sapiens 83-88 17482291-4 2007 Wortmannin, SH-5, and rapamycin significantly blocked PDGF-stimulated induction of SphK1 mRNA and protein expression, indicating a regulatory role of the PI3K/AKT/mTOR pathway in SphK1 expression. Sirolimus 22-31 sphingosine kinase 1 Homo sapiens 179-184 17388800-7 2007 IGF-I activated SphK, leading to sphingosine-1-phosphate (S1P) formation. sphingosine 1-phosphate 33-56 sphingosine kinase 1 Homo sapiens 16-20 17265031-4 2007 injection, were used to investigate the glucose-lowering effects of SPHK1 in vivo. Glucose 40-47 sphingosine kinase 1 Homo sapiens 68-73 17388800-7 2007 IGF-I activated SphK, leading to sphingosine-1-phosphate (S1P) formation. sphingosine 1-phosphate 58-61 sphingosine kinase 1 Homo sapiens 16-20 17376388-1 2007 Generation of sphingosine-1-phosphate by sphingosine kinases (SphKs) promotes allergic inflammatory diseases, but the roles of the individual SphK isoforms are unclear. sphingosine 1-phosphate 14-37 sphingosine kinase 1 Homo sapiens 62-66 16926156-4 2006 Treatment with IGF-1 or IGF-2 promoted translocation of green fluorescent protein (GFP)-tagged sphingosine kinase (SK) 1 from the cytosol to the plasma membrane, increased endogenous SK activity within 30 s of stimulation, and caused a statistically significant increase in intracellular and extracellular sphingosine 1-phosphate (S1P) concentration. sphingosine 1-phosphate 306-329 sphingosine kinase 1 Homo sapiens 95-120 17346996-2 2007 Stimulation of mast cells by allergens induces two mammalian sphingosine kinases (Sphk1 and Sphk2) to produce sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 110-133 sphingosine kinase 1 Homo sapiens 82-87 17127411-7 2007 PI3K was mapped upstream of SphK, since wortmannin could block SphK activation by S1P. Wortmannin 40-50 sphingosine kinase 1 Homo sapiens 28-32 17127411-7 2007 PI3K was mapped upstream of SphK, since wortmannin could block SphK activation by S1P. Wortmannin 40-50 sphingosine kinase 1 Homo sapiens 63-67 17127411-10 2007 Thus, we conclude that Fas death signaling aberration in RA LCLs is caused by extracellular S1P, which triggers PI3K-dependent SphK over-activity through a Gi protein-coupled receptor-mediated signaling cascade. ammonium ferrous sulfate 23-26 sphingosine kinase 1 Homo sapiens 127-131 17159597-4 2007 Sphingosine kinase-1 is a crucial regulator of this two-pan balance, because it produces the prosurvival sphingosine 1-phosphate, and reduces the content of both ceramide and sphingosine, the proapoptotic sphingolipids. sphingosine 1-phosphate 105-128 sphingosine kinase 1 Homo sapiens 0-20 17159597-4 2007 Sphingosine kinase-1 is a crucial regulator of this two-pan balance, because it produces the prosurvival sphingosine 1-phosphate, and reduces the content of both ceramide and sphingosine, the proapoptotic sphingolipids. Ceramides 162-170 sphingosine kinase 1 Homo sapiens 0-20 17159597-4 2007 Sphingosine kinase-1 is a crucial regulator of this two-pan balance, because it produces the prosurvival sphingosine 1-phosphate, and reduces the content of both ceramide and sphingosine, the proapoptotic sphingolipids. Sphingosine 105-116 sphingosine kinase 1 Homo sapiens 0-20 17159597-4 2007 Sphingosine kinase-1 is a crucial regulator of this two-pan balance, because it produces the prosurvival sphingosine 1-phosphate, and reduces the content of both ceramide and sphingosine, the proapoptotic sphingolipids. Sphingolipids 205-218 sphingosine kinase 1 Homo sapiens 0-20 17159597-5 2007 Sphingosine kinase-1 controls the levels of sphingolipids having opposite effects on cell survival/death, its gene was found to be of oncogenic nature, its mRNA is overexpressed in many solid tumors, its overexpression protects cells from apoptosis and its activity is decreased during anticancer treatments. Sphingolipids 44-57 sphingosine kinase 1 Homo sapiens 0-20 17124500-1 2006 Sphingosine 1-phosphate (S1P), produced by sphingosine kinase (SPHK), acts both by intracellular and extracellular modes. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 43-61 17124500-1 2006 Sphingosine 1-phosphate (S1P), produced by sphingosine kinase (SPHK), acts both by intracellular and extracellular modes. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 63-67 17124500-1 2006 Sphingosine 1-phosphate (S1P), produced by sphingosine kinase (SPHK), acts both by intracellular and extracellular modes. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 43-61 17124500-1 2006 Sphingosine 1-phosphate (S1P), produced by sphingosine kinase (SPHK), acts both by intracellular and extracellular modes. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 63-67 16926156-6 2006 Treating cells with dimethylsphingosine, silencing SK1 expression by RNA interference, and blocking endogenous S1P receptors with the competitive antagonist VPC23019 all significantly inhibited IGF-stimulated ERK1/2 activation, suggesting that IGFs elicit G protein-dependent ERK1/2 activation by stimulating SK1-dependent transactivation of S1P receptors. VPC23019 157-165 sphingosine kinase 1 Homo sapiens 309-312 17064696-5 2006 The efficacy of cleavage of SK1 at arginine 199, however, was not affected. Arginine 35-43 sphingosine kinase 1 Homo sapiens 28-31 17064696-6 2006 These studies demonstrate that SK1 is cleaved by cathepsin B in a sequential manner after basic amino acids, and that the initial cleavages at the two identified sites occur independently of each other. Amino Acids, Basic 90-107 sphingosine kinase 1 Homo sapiens 31-34 16926156-4 2006 Treatment with IGF-1 or IGF-2 promoted translocation of green fluorescent protein (GFP)-tagged sphingosine kinase (SK) 1 from the cytosol to the plasma membrane, increased endogenous SK activity within 30 s of stimulation, and caused a statistically significant increase in intracellular and extracellular sphingosine 1-phosphate (S1P) concentration. sphingosine 1-phosphate 331-334 sphingosine kinase 1 Homo sapiens 95-120 16508940-12 2006 Competitive inhibitors of SPHK reversed the resistance of RA LCLs to Fas-induced apoptosis. ammonium ferrous sulfate 69-72 sphingosine kinase 1 Homo sapiens 26-30 16529909-0 2006 De novo biosynthesis of dihydrosphingosine-1-phosphate by sphingosine kinase 1 in mammalian cells. dihydrosphingosine 1-phosphate 24-54 sphingosine kinase 1 Homo sapiens 58-78 16529909-1 2006 Sphingosine kinase 1 (SK1) is one of the two known kinases, which generates sphingosine-1-phosphate (S1P), a potent endogenous lipid mediator involved in cell survival, proliferation, and cell-cell interactions. sphingosine 1-phosphate 76-99 sphingosine kinase 1 Homo sapiens 0-20 16529909-1 2006 Sphingosine kinase 1 (SK1) is one of the two known kinases, which generates sphingosine-1-phosphate (S1P), a potent endogenous lipid mediator involved in cell survival, proliferation, and cell-cell interactions. sphingosine 1-phosphate 76-99 sphingosine kinase 1 Homo sapiens 22-25 16529909-3 2006 Using a highly selective and sensitive LC-MS/MS approach, here we show that SK1 overexpression, but not SK2, in different primary cells and cultured cell lines results in predominant upregulation of the synthesis of dihydrosphingosine-1-phosphate (DHS1P) compared to S1P. dihydrosphingosine 1-phosphate 216-246 sphingosine kinase 1 Homo sapiens 76-79 16529909-3 2006 Using a highly selective and sensitive LC-MS/MS approach, here we show that SK1 overexpression, but not SK2, in different primary cells and cultured cell lines results in predominant upregulation of the synthesis of dihydrosphingosine-1-phosphate (DHS1P) compared to S1P. dihydrosphingosine 1-phosphate 248-253 sphingosine kinase 1 Homo sapiens 76-79 16529909-4 2006 Stable isotope pulse-labeling experiments in conjunction with LC-MS/MS quantitation of different sphingolipids demonstrated strong interference of overexpressed SK1 with the de novo sphingolipid biosynthesis by deviating metabolic flow of newly formed sphingoid bases from ceramide formation toward the synthesis of DHS1P. Sphingolipids 97-110 sphingosine kinase 1 Homo sapiens 161-164 16529909-4 2006 Stable isotope pulse-labeling experiments in conjunction with LC-MS/MS quantitation of different sphingolipids demonstrated strong interference of overexpressed SK1 with the de novo sphingolipid biosynthesis by deviating metabolic flow of newly formed sphingoid bases from ceramide formation toward the synthesis of DHS1P. Sphingolipids 97-109 sphingosine kinase 1 Homo sapiens 161-164 16529909-4 2006 Stable isotope pulse-labeling experiments in conjunction with LC-MS/MS quantitation of different sphingolipids demonstrated strong interference of overexpressed SK1 with the de novo sphingolipid biosynthesis by deviating metabolic flow of newly formed sphingoid bases from ceramide formation toward the synthesis of DHS1P. sphingoid bases 252-267 sphingosine kinase 1 Homo sapiens 161-164 16529909-4 2006 Stable isotope pulse-labeling experiments in conjunction with LC-MS/MS quantitation of different sphingolipids demonstrated strong interference of overexpressed SK1 with the de novo sphingolipid biosynthesis by deviating metabolic flow of newly formed sphingoid bases from ceramide formation toward the synthesis of DHS1P. Ceramides 273-281 sphingosine kinase 1 Homo sapiens 161-164 16529909-4 2006 Stable isotope pulse-labeling experiments in conjunction with LC-MS/MS quantitation of different sphingolipids demonstrated strong interference of overexpressed SK1 with the de novo sphingolipid biosynthesis by deviating metabolic flow of newly formed sphingoid bases from ceramide formation toward the synthesis of DHS1P. dihydrosphingosine 1-phosphate 316-321 sphingosine kinase 1 Homo sapiens 161-164 16529909-8 2006 Furthermore, infection of human bronchial epithelial cells (HBEpC) with RSV A-2 virus increased SK1-mediated synthesis of DHS1P and S1P, whereas TNF-alpha enhanced only S1P production in HPAEC. dihydrosphingosine 1-phosphate 122-127 sphingosine kinase 1 Homo sapiens 96-99 16529909-9 2006 These findings uncover a new functional role for SK1, which can control survival/death (DHS1P-S1P/ceramides) balance by targeting sphingolipid de novo biosynthesis and selectively generating DHS1P at a metabolic step preceding ceramide formation. dihydrosphingosine 1-phosphate 88-93 sphingosine kinase 1 Homo sapiens 49-52 16529909-9 2006 These findings uncover a new functional role for SK1, which can control survival/death (DHS1P-S1P/ceramides) balance by targeting sphingolipid de novo biosynthesis and selectively generating DHS1P at a metabolic step preceding ceramide formation. Ceramides 98-107 sphingosine kinase 1 Homo sapiens 49-52 16529909-9 2006 These findings uncover a new functional role for SK1, which can control survival/death (DHS1P-S1P/ceramides) balance by targeting sphingolipid de novo biosynthesis and selectively generating DHS1P at a metabolic step preceding ceramide formation. Sphingolipids 130-142 sphingosine kinase 1 Homo sapiens 49-52 16529909-9 2006 These findings uncover a new functional role for SK1, which can control survival/death (DHS1P-S1P/ceramides) balance by targeting sphingolipid de novo biosynthesis and selectively generating DHS1P at a metabolic step preceding ceramide formation. dihydrosphingosine 1-phosphate 191-196 sphingosine kinase 1 Homo sapiens 49-52 16529909-9 2006 These findings uncover a new functional role for SK1, which can control survival/death (DHS1P-S1P/ceramides) balance by targeting sphingolipid de novo biosynthesis and selectively generating DHS1P at a metabolic step preceding ceramide formation. Ceramides 98-106 sphingosine kinase 1 Homo sapiens 49-52 16928321-1 2006 Sphingosine 1-phosphate (S1P), which can be impacted by different growth factors through sphingosine kinase (SphK), is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 89-107 16928321-1 2006 Sphingosine 1-phosphate (S1P), which can be impacted by different growth factors through sphingosine kinase (SphK), is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 109-113 16516161-0 2006 High expression of sphingosine kinase 1 and S1P receptors in chemotherapy-resistant prostate cancer PC3 cells and their camptothecin-induced up-regulation. Camptothecin 120-132 sphingosine kinase 1 Homo sapiens 19-37 16415355-2 2006 Here we show that overexpression of sphingosine kinase 1 (SK1), an enzyme responsible for the production of sphingosine 1-phosphate (S1P), in MCF-7 cells stimulates autophagy by increasing the formation of LC3-positive autophagosomes and the rate of proteolysis sensitive to the autophagy inhibitor 3-methyladenine. sphingosine 1-phosphate 108-131 sphingosine kinase 1 Homo sapiens 36-56 16415355-2 2006 Here we show that overexpression of sphingosine kinase 1 (SK1), an enzyme responsible for the production of sphingosine 1-phosphate (S1P), in MCF-7 cells stimulates autophagy by increasing the formation of LC3-positive autophagosomes and the rate of proteolysis sensitive to the autophagy inhibitor 3-methyladenine. sphingosine 1-phosphate 108-131 sphingosine kinase 1 Homo sapiens 58-61 16415355-2 2006 Here we show that overexpression of sphingosine kinase 1 (SK1), an enzyme responsible for the production of sphingosine 1-phosphate (S1P), in MCF-7 cells stimulates autophagy by increasing the formation of LC3-positive autophagosomes and the rate of proteolysis sensitive to the autophagy inhibitor 3-methyladenine. 3-methyladenine 299-314 sphingosine kinase 1 Homo sapiens 36-56 16415355-2 2006 Here we show that overexpression of sphingosine kinase 1 (SK1), an enzyme responsible for the production of sphingosine 1-phosphate (S1P), in MCF-7 cells stimulates autophagy by increasing the formation of LC3-positive autophagosomes and the rate of proteolysis sensitive to the autophagy inhibitor 3-methyladenine. 3-methyladenine 299-314 sphingosine kinase 1 Homo sapiens 58-61 16508940-7 2006 The functional role of sphingosine kinase (SPHK) was determined by measuring its enzymatic activity, by quantifying the levels of its product, sphingosine 1-phosphate (S1P), and by investigating the ability of the SPHK inhibitor N,N-dimethylsphingosine and isozyme-specific small interfering RNA (siRNA) oligonucleotides to reverse signaling aberrations. sphingosine 1-phosphate 143-166 sphingosine kinase 1 Homo sapiens 23-41 16508940-7 2006 The functional role of sphingosine kinase (SPHK) was determined by measuring its enzymatic activity, by quantifying the levels of its product, sphingosine 1-phosphate (S1P), and by investigating the ability of the SPHK inhibitor N,N-dimethylsphingosine and isozyme-specific small interfering RNA (siRNA) oligonucleotides to reverse signaling aberrations. sphingosine 1-phosphate 143-166 sphingosine kinase 1 Homo sapiens 43-47 16508940-7 2006 The functional role of sphingosine kinase (SPHK) was determined by measuring its enzymatic activity, by quantifying the levels of its product, sphingosine 1-phosphate (S1P), and by investigating the ability of the SPHK inhibitor N,N-dimethylsphingosine and isozyme-specific small interfering RNA (siRNA) oligonucleotides to reverse signaling aberrations. sphingosine 1-phosphate 168-171 sphingosine kinase 1 Homo sapiens 23-41 16508940-7 2006 The functional role of sphingosine kinase (SPHK) was determined by measuring its enzymatic activity, by quantifying the levels of its product, sphingosine 1-phosphate (S1P), and by investigating the ability of the SPHK inhibitor N,N-dimethylsphingosine and isozyme-specific small interfering RNA (siRNA) oligonucleotides to reverse signaling aberrations. sphingosine 1-phosphate 168-171 sphingosine kinase 1 Homo sapiens 43-47 16508940-7 2006 The functional role of sphingosine kinase (SPHK) was determined by measuring its enzymatic activity, by quantifying the levels of its product, sphingosine 1-phosphate (S1P), and by investigating the ability of the SPHK inhibitor N,N-dimethylsphingosine and isozyme-specific small interfering RNA (siRNA) oligonucleotides to reverse signaling aberrations. N,N-dimethylsphingosine 229-252 sphingosine kinase 1 Homo sapiens 23-41 16508940-7 2006 The functional role of sphingosine kinase (SPHK) was determined by measuring its enzymatic activity, by quantifying the levels of its product, sphingosine 1-phosphate (S1P), and by investigating the ability of the SPHK inhibitor N,N-dimethylsphingosine and isozyme-specific small interfering RNA (siRNA) oligonucleotides to reverse signaling aberrations. N,N-dimethylsphingosine 229-252 sphingosine kinase 1 Homo sapiens 43-47 16508940-7 2006 The functional role of sphingosine kinase (SPHK) was determined by measuring its enzymatic activity, by quantifying the levels of its product, sphingosine 1-phosphate (S1P), and by investigating the ability of the SPHK inhibitor N,N-dimethylsphingosine and isozyme-specific small interfering RNA (siRNA) oligonucleotides to reverse signaling aberrations. Oligonucleotides 304-320 sphingosine kinase 1 Homo sapiens 23-41 16508940-7 2006 The functional role of sphingosine kinase (SPHK) was determined by measuring its enzymatic activity, by quantifying the levels of its product, sphingosine 1-phosphate (S1P), and by investigating the ability of the SPHK inhibitor N,N-dimethylsphingosine and isozyme-specific small interfering RNA (siRNA) oligonucleotides to reverse signaling aberrations. Oligonucleotides 304-320 sphingosine kinase 1 Homo sapiens 43-47 16571380-2 2006 In this study, we show that in the human arterial endothelial cell line EA.hy 926 histamine induces a time-dependent upregulation of the SK-1 mRNA and protein expression which is followed by increased SK-1 activity. Histamine 82-91 sphingosine kinase 1 Homo sapiens 137-141 16571380-2 2006 In this study, we show that in the human arterial endothelial cell line EA.hy 926 histamine induces a time-dependent upregulation of the SK-1 mRNA and protein expression which is followed by increased SK-1 activity. Histamine 82-91 sphingosine kinase 1 Homo sapiens 201-205 16571380-3 2006 A similar upregulation of SK-1 is also observed with the direct protein kinase C activator 12-O-tetradecanoylphorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 91-127 sphingosine kinase 1 Homo sapiens 26-30 16571380-3 2006 A similar upregulation of SK-1 is also observed with the direct protein kinase C activator 12-O-tetradecanoylphorbol-13-acetate (TPA). Tetradecanoylphorbol Acetate 129-132 sphingosine kinase 1 Homo sapiens 26-30 16571380-5 2006 The increased SK-1 protein expression is due to stimulated de novo synthesis since cycloheximide inhibited the delayed SK-1 protein upregulation. Cycloheximide 83-96 sphingosine kinase 1 Homo sapiens 14-18 16571380-5 2006 The increased SK-1 protein expression is due to stimulated de novo synthesis since cycloheximide inhibited the delayed SK-1 protein upregulation. Cycloheximide 83-96 sphingosine kinase 1 Homo sapiens 119-123 16571380-6 2006 Moreover, the increased SK-1 mRNA expression results from an increased promoter activation by histamine and TPA. Histamine 94-103 sphingosine kinase 1 Homo sapiens 24-28 16571380-6 2006 Moreover, the increased SK-1 mRNA expression results from an increased promoter activation by histamine and TPA. Tetradecanoylphorbol Acetate 108-111 sphingosine kinase 1 Homo sapiens 24-28 16571380-7 2006 In mechanistic terms, the transcriptional upregulation of SK-1 is dependent on PKC and the extracellular signal-regulated protein kinase (ERK) cascade since staurosporine and the MEK inhibitor U0126 abolish the TPA-induced SK-1 induction. Staurosporine 157-170 sphingosine kinase 1 Homo sapiens 58-62 16571380-7 2006 In mechanistic terms, the transcriptional upregulation of SK-1 is dependent on PKC and the extracellular signal-regulated protein kinase (ERK) cascade since staurosporine and the MEK inhibitor U0126 abolish the TPA-induced SK-1 induction. Staurosporine 157-170 sphingosine kinase 1 Homo sapiens 223-227 16571380-7 2006 In mechanistic terms, the transcriptional upregulation of SK-1 is dependent on PKC and the extracellular signal-regulated protein kinase (ERK) cascade since staurosporine and the MEK inhibitor U0126 abolish the TPA-induced SK-1 induction. U 0126 193-198 sphingosine kinase 1 Homo sapiens 58-62 16571380-7 2006 In mechanistic terms, the transcriptional upregulation of SK-1 is dependent on PKC and the extracellular signal-regulated protein kinase (ERK) cascade since staurosporine and the MEK inhibitor U0126 abolish the TPA-induced SK-1 induction. U 0126 193-198 sphingosine kinase 1 Homo sapiens 223-227 16571380-7 2006 In mechanistic terms, the transcriptional upregulation of SK-1 is dependent on PKC and the extracellular signal-regulated protein kinase (ERK) cascade since staurosporine and the MEK inhibitor U0126 abolish the TPA-induced SK-1 induction. Tetradecanoylphorbol Acetate 211-214 sphingosine kinase 1 Homo sapiens 58-62 16571380-9 2006 Parallel to the induction of SK-1, histamine and TPA stimulate an increased migration of endothelial cells, which is prevented by depletion of the SK-1 by small interfering RNA (siRNA). Histamine 35-44 sphingosine kinase 1 Homo sapiens 147-151 16571380-9 2006 Parallel to the induction of SK-1, histamine and TPA stimulate an increased migration of endothelial cells, which is prevented by depletion of the SK-1 by small interfering RNA (siRNA). Tetradecanoylphorbol Acetate 49-52 sphingosine kinase 1 Homo sapiens 147-151 16571380-11 2006 Interestingly, only depletion of PKC-alpha leads to a complete loss of TPA- and histamine-triggered SK-1 induction and cell migration. Tetradecanoylphorbol Acetate 71-74 sphingosine kinase 1 Homo sapiens 100-104 16571380-11 2006 Interestingly, only depletion of PKC-alpha leads to a complete loss of TPA- and histamine-triggered SK-1 induction and cell migration. Histamine 80-89 sphingosine kinase 1 Homo sapiens 100-104 16507765-0 2006 Loss of sphingosine kinase-1 activates the intrinsic pathway of programmed cell death: modulation of sphingolipid levels and the induction of apoptosis. Sphingolipids 101-113 sphingosine kinase 1 Homo sapiens 8-28 16507765-11 2006 SK1 knockdown also induced significant increases in ceramide levels in whole cells and in mitochondria enriched fractions of cells. Ceramides 52-60 sphingosine kinase 1 Homo sapiens 0-3 16507765-12 2006 Inhibition of de novo sphingolipid biosynthesis with myriocin significantly attenuated Bax oligomerization and downstream caspase activation after SK1 loss. Sphingolipids 22-34 sphingosine kinase 1 Homo sapiens 147-150 16507765-12 2006 Inhibition of de novo sphingolipid biosynthesis with myriocin significantly attenuated Bax oligomerization and downstream caspase activation after SK1 loss. thermozymocidin 53-61 sphingosine kinase 1 Homo sapiens 147-150 16507765-13 2006 These studies for the first time implicate endogenous SK1 as an important survival enzyme in MCF-7 cells and link the biological consequences of knocking down the enzyme to its biochemical role as a regulator of sphingolipid metabolism. Sphingolipids 212-224 sphingosine kinase 1 Homo sapiens 54-57 16516816-5 2006 In this study we examined the effect of oxLDL-IC on sphingosine kinase 1 (SK1), an enzyme implicated in mediating pro-survival and inflammatory responses through the generation of the signaling molecule sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 203-226 sphingosine kinase 1 Homo sapiens 52-72 16516816-5 2006 In this study we examined the effect of oxLDL-IC on sphingosine kinase 1 (SK1), an enzyme implicated in mediating pro-survival and inflammatory responses through the generation of the signaling molecule sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 203-226 sphingosine kinase 1 Homo sapiens 74-77 16928321-1 2006 Sphingosine 1-phosphate (S1P), which can be impacted by different growth factors through sphingosine kinase (SphK), is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. Sphingolipids 163-175 sphingosine kinase 1 Homo sapiens 89-107 16928321-1 2006 Sphingosine 1-phosphate (S1P), which can be impacted by different growth factors through sphingosine kinase (SphK), is a bioactive lipid produced by metabolism of sphingolipid with various biological responses. Sphingolipids 163-175 sphingosine kinase 1 Homo sapiens 109-113 16522638-6 2006 Specifically, Phe197 and Leu198 are critically involved in the interaction because a version of hSK1 incorporating mutations of both Phe197 --> Ala and Leu198 --> Gln failed to bind CaM. Alanine 147-150 sphingosine kinase 1 Homo sapiens 96-100 16522638-6 2006 Specifically, Phe197 and Leu198 are critically involved in the interaction because a version of hSK1 incorporating mutations of both Phe197 --> Ala and Leu198 --> Gln failed to bind CaM. Glutamine 169-172 sphingosine kinase 1 Homo sapiens 96-100 16319132-6 2006 In addition, it was found that 1) down-regulation of SK1 in HT-29 human colon cancer cells by small interfering RNA (siRNA) decreases COX-2 expression and PGE2 production; 2) overexpression of SK1 in RIE-1 rat intestinal epithelial cells induces COX-2 expression; and 3) S1P stimulates COX-2 expression and PGE2 production in HT-29 cells. Dinoprostone 155-159 sphingosine kinase 1 Homo sapiens 53-56 16319132-6 2006 In addition, it was found that 1) down-regulation of SK1 in HT-29 human colon cancer cells by small interfering RNA (siRNA) decreases COX-2 expression and PGE2 production; 2) overexpression of SK1 in RIE-1 rat intestinal epithelial cells induces COX-2 expression; and 3) S1P stimulates COX-2 expression and PGE2 production in HT-29 cells. Dinoprostone 155-159 sphingosine kinase 1 Homo sapiens 193-196 16319132-6 2006 In addition, it was found that 1) down-regulation of SK1 in HT-29 human colon cancer cells by small interfering RNA (siRNA) decreases COX-2 expression and PGE2 production; 2) overexpression of SK1 in RIE-1 rat intestinal epithelial cells induces COX-2 expression; and 3) S1P stimulates COX-2 expression and PGE2 production in HT-29 cells. Dinoprostone 307-311 sphingosine kinase 1 Homo sapiens 53-56 16319132-6 2006 In addition, it was found that 1) down-regulation of SK1 in HT-29 human colon cancer cells by small interfering RNA (siRNA) decreases COX-2 expression and PGE2 production; 2) overexpression of SK1 in RIE-1 rat intestinal epithelial cells induces COX-2 expression; and 3) S1P stimulates COX-2 expression and PGE2 production in HT-29 cells. Dinoprostone 307-311 sphingosine kinase 1 Homo sapiens 193-196 16508940-13 2006 Additionally, resistance to Fas-mediated signaling was reversed by siRNA oligonucleotides specific for SPHK-1 but not by oligonucleotides specific for SPHK-2. ammonium ferrous sulfate 28-31 sphingosine kinase 1 Homo sapiens 103-109 16508940-13 2006 Additionally, resistance to Fas-mediated signaling was reversed by siRNA oligonucleotides specific for SPHK-1 but not by oligonucleotides specific for SPHK-2. Oligonucleotides 73-89 sphingosine kinase 1 Homo sapiens 103-109 16278291-1 2006 Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes formation of important regulators of inter- and intracellular signaling, sphingosine-1 phosphate (S1P), and dihydrosphingosine 1-phosphate (dhS1P). sphingosine 1-phosphate 142-165 sphingosine kinase 1 Homo sapiens 0-18 16278291-1 2006 Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes formation of important regulators of inter- and intracellular signaling, sphingosine-1 phosphate (S1P), and dihydrosphingosine 1-phosphate (dhS1P). sphingosine 1-phosphate 142-165 sphingosine kinase 1 Homo sapiens 20-24 16278291-1 2006 Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes formation of important regulators of inter- and intracellular signaling, sphingosine-1 phosphate (S1P), and dihydrosphingosine 1-phosphate (dhS1P). dihydrosphingosine 1-phosphate 177-207 sphingosine kinase 1 Homo sapiens 0-18 16278291-1 2006 Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes formation of important regulators of inter- and intracellular signaling, sphingosine-1 phosphate (S1P), and dihydrosphingosine 1-phosphate (dhS1P). dihydrosphingosine 1-phosphate 177-207 sphingosine kinase 1 Homo sapiens 20-24 16278291-1 2006 Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes formation of important regulators of inter- and intracellular signaling, sphingosine-1 phosphate (S1P), and dihydrosphingosine 1-phosphate (dhS1P). dihydrosphingosine 1-phosphate 209-214 sphingosine kinase 1 Homo sapiens 0-18 16278291-1 2006 Sphingosine kinase (SphK) is a conserved lipid kinase that catalyzes formation of important regulators of inter- and intracellular signaling, sphingosine-1 phosphate (S1P), and dihydrosphingosine 1-phosphate (dhS1P). dihydrosphingosine 1-phosphate 209-214 sphingosine kinase 1 Homo sapiens 20-24 16414307-5 2005 Moreover, dexamethasone also suppressed EGF-induced SK-1 mRNA expression and activity which is reversed by the glucocorticoid receptor antagonist RU486. Dexamethasone 10-23 sphingosine kinase 1 Homo sapiens 52-56 16698674-2 2006 Sphingosine kinase (SphK) and its product sphingosine-1-phosphate (S1P) play an important role in the regulation of immune cells and cancer, affecting processes such as differentiation, growth or migration. sphingosine 1-phosphate 42-65 sphingosine kinase 1 Homo sapiens 0-18 16698674-2 2006 Sphingosine kinase (SphK) and its product sphingosine-1-phosphate (S1P) play an important role in the regulation of immune cells and cancer, affecting processes such as differentiation, growth or migration. sphingosine 1-phosphate 42-65 sphingosine kinase 1 Homo sapiens 20-24 16698674-2 2006 Sphingosine kinase (SphK) and its product sphingosine-1-phosphate (S1P) play an important role in the regulation of immune cells and cancer, affecting processes such as differentiation, growth or migration. sphingosine 1-phosphate 67-70 sphingosine kinase 1 Homo sapiens 0-18 16698674-2 2006 Sphingosine kinase (SphK) and its product sphingosine-1-phosphate (S1P) play an important role in the regulation of immune cells and cancer, affecting processes such as differentiation, growth or migration. sphingosine 1-phosphate 67-70 sphingosine kinase 1 Homo sapiens 20-24 16698674-8 2006 The SphK-inhibitor dihydro-sphingosine (DHS) reduced migration of iDC but not of mDC. safingol 19-38 sphingosine kinase 1 Homo sapiens 4-8 16698674-8 2006 The SphK-inhibitor dihydro-sphingosine (DHS) reduced migration of iDC but not of mDC. safingol 40-43 sphingosine kinase 1 Homo sapiens 4-8 16243846-4 2005 In vitro measurements showed that hSK1 selectively bound phosphatidylserine over other anionic phospholipids and strongly preferred the plasma membrane-mimicking membrane to other cellular membrane mimetics. Phosphatidylserines 57-75 sphingosine kinase 1 Homo sapiens 34-38 16243846-4 2005 In vitro measurements showed that hSK1 selectively bound phosphatidylserine over other anionic phospholipids and strongly preferred the plasma membrane-mimicking membrane to other cellular membrane mimetics. Phospholipids 95-108 sphingosine kinase 1 Homo sapiens 34-38 16281067-1 2006 We examined the involvement of sphingosine kinase-1, a critical regulator of the sphingolipid balance, in susceptibility to antineoplastic agents of either sensitive or multidrug-resistant acute myeloid leukemia cells. Sphingolipids 81-93 sphingosine kinase 1 Homo sapiens 31-51 16281067-4 2006 In contrast, chemoresistant HL-60/ Doxo and HL-60/VP16 had sustained sphingosine kinase-1 activity and did not produce ceramide during treatment. Desoxycorticosterone Acetate 35-39 sphingosine kinase 1 Homo sapiens 69-89 16281067-6 2006 Incubation with cell-permeable ceramide of chemoresistant cells led to a sphingosine kinase-1 inhibition and apoptosis both prevented by sphingosine kinase-1 over-expression. Ceramides 31-39 sphingosine kinase 1 Homo sapiens 73-93 16281067-6 2006 Incubation with cell-permeable ceramide of chemoresistant cells led to a sphingosine kinase-1 inhibition and apoptosis both prevented by sphingosine kinase-1 over-expression. Ceramides 31-39 sphingosine kinase 1 Homo sapiens 137-157 16414307-5 2005 Moreover, dexamethasone also suppressed EGF-induced SK-1 mRNA expression and activity which is reversed by the glucocorticoid receptor antagonist RU486. Mifepristone 146-151 sphingosine kinase 1 Homo sapiens 52-56 16414307-8 2005 When cells are depleted of SK-1, but not SK-2, by siRNA transfection or by dexamethasone treatment, EGF-induced proliferation and migration are drastically reduced. Dexamethasone 75-88 sphingosine kinase 1 Homo sapiens 27-31 16357178-2 2005 We took advantage of the known differential effect of camptothecin and docetaxel on human PC-3 and LNCaP prostate cancer cells to determine their effect on sphingosine kinase-1 (SphK1) activity and subsequent ceramide/sphingosine 1-phosphate (S1P) balance in relation with cell survival. Camptothecin 54-66 sphingosine kinase 1 Homo sapiens 156-176 16357178-2 2005 We took advantage of the known differential effect of camptothecin and docetaxel on human PC-3 and LNCaP prostate cancer cells to determine their effect on sphingosine kinase-1 (SphK1) activity and subsequent ceramide/sphingosine 1-phosphate (S1P) balance in relation with cell survival. Camptothecin 54-66 sphingosine kinase 1 Homo sapiens 178-183 16357178-2 2005 We took advantage of the known differential effect of camptothecin and docetaxel on human PC-3 and LNCaP prostate cancer cells to determine their effect on sphingosine kinase-1 (SphK1) activity and subsequent ceramide/sphingosine 1-phosphate (S1P) balance in relation with cell survival. Docetaxel 71-80 sphingosine kinase 1 Homo sapiens 156-176 16357178-2 2005 We took advantage of the known differential effect of camptothecin and docetaxel on human PC-3 and LNCaP prostate cancer cells to determine their effect on sphingosine kinase-1 (SphK1) activity and subsequent ceramide/sphingosine 1-phosphate (S1P) balance in relation with cell survival. Docetaxel 71-80 sphingosine kinase 1 Homo sapiens 178-183 16118219-10 2005 Our results demonstrate that SphK1 and SphK2 have opposing roles in the regulation of ceramide biosynthesis and suggest that the location of sphingosine 1-phosphate production dictates its functions. Ceramides 86-94 sphingosine kinase 1 Homo sapiens 29-34 16118219-0 2005 SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism. Sphingolipids 74-86 sphingosine kinase 1 Homo sapiens 0-5 16103110-1 2005 Sphingosine kinase (SPHK) 1 is implicated in the regulation of cell proliferation and anti-apoptotic processes by catalyzing the formation of an important bioactive messenger, sphingosine 1-phosphate. sphingosine 1-phosphate 176-199 sphingosine kinase 1 Homo sapiens 0-27 16194537-5 2005 Downregulation of SphK1 in MCF-7 cells reduced EGF- and serum-stimulated growth and enhanced sensitivity to doxorubicin, a potent chemotherapeutic agent. Doxorubicin 108-119 sphingosine kinase 1 Homo sapiens 18-23 16038795-3 2005 This transient activation of SPHK1 was found to be required for cytokine-induced COX-2 transcription and PGE2 production, since not only specific siRNA (abolishing both basal and induced SPHK1 enzyme activity), but also a dominant-negative SPHK1 mutant (suppressing induced SPHK1 activity only) both reduced COX-2 and PGE2. Dinoprostone 105-109 sphingosine kinase 1 Homo sapiens 29-34 16038795-3 2005 This transient activation of SPHK1 was found to be required for cytokine-induced COX-2 transcription and PGE2 production, since not only specific siRNA (abolishing both basal and induced SPHK1 enzyme activity), but also a dominant-negative SPHK1 mutant (suppressing induced SPHK1 activity only) both reduced COX-2 and PGE2. Dinoprostone 318-322 sphingosine kinase 1 Homo sapiens 29-34 16038795-6 2005 Inhibition of basal SPHK1 activity by N,N-dimethylsphingosine or by downregulation of its expression using siRNA induced spontaneous apoptosis in A549 cells, an effect that can be explained through interference with constitutive NFkappaB activity in this cell type. N,N-dimethylsphingosine 38-61 sphingosine kinase 1 Homo sapiens 20-25 16246121-6 2005 Additionally, we describe a model whereby LPP2, but not LPP3, may be functionally linked to the phospholipase D1-derived PA-dependent recruitment of sphingosine kinase 1 to the perinuclear compartment. Phosphatidic Acids 121-123 sphingosine kinase 1 Homo sapiens 149-169 16313513-4 2005 Immunocytochemistry and confocal microscopy indicated that NT-3 induces translocation of SphK1 from the cytoplasm to the plasma membrane of oligodendrocytes, a process accompanied by increased SphK1 activity in the membrane fraction where its substrate sphingosine resides. Sphingosine 253-264 sphingosine kinase 1 Homo sapiens 89-94 16313513-6 2005 Remarkably, the capacity of NT-3 to protect oligodendrocyte progenitors from apoptotic cell death induced by growth factor deprivation was abolished by down-regulating the expression of SphK1, as assessed by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. deoxyuridine triphosphate 246-250 sphingosine kinase 1 Homo sapiens 186-191 16118219-10 2005 Our results demonstrate that SphK1 and SphK2 have opposing roles in the regulation of ceramide biosynthesis and suggest that the location of sphingosine 1-phosphate production dictates its functions. sphingosine 1-phosphate 141-164 sphingosine kinase 1 Homo sapiens 29-34 15951439-1 2005 Sphingosine 1-phosphate (S1P), produced by two sphingosine kinase isoenzymes, denoted SphK1 and SphK2, is the ligand for a family of five specific G protein-coupled receptors that regulate cytoskeletal rearrangements and cell motility. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 86-91 15951439-1 2005 Sphingosine 1-phosphate (S1P), produced by two sphingosine kinase isoenzymes, denoted SphK1 and SphK2, is the ligand for a family of five specific G protein-coupled receptors that regulate cytoskeletal rearrangements and cell motility. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 86-91 15993704-5 2005 EGF also activates sphingosine kinase-1 (SPHK-1), which converts sphingosine to sphingosine-1-phosphate, and its inhibition with dimethyl sphingosine (DMS) increased trophoblast death. Sphingosine 19-30 sphingosine kinase 1 Homo sapiens 41-47 15993704-5 2005 EGF also activates sphingosine kinase-1 (SPHK-1), which converts sphingosine to sphingosine-1-phosphate, and its inhibition with dimethyl sphingosine (DMS) increased trophoblast death. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 19-39 15993704-5 2005 EGF also activates sphingosine kinase-1 (SPHK-1), which converts sphingosine to sphingosine-1-phosphate, and its inhibition with dimethyl sphingosine (DMS) increased trophoblast death. sphingosine 1-phosphate 80-103 sphingosine kinase 1 Homo sapiens 41-47 15993704-5 2005 EGF also activates sphingosine kinase-1 (SPHK-1), which converts sphingosine to sphingosine-1-phosphate, and its inhibition with dimethyl sphingosine (DMS) increased trophoblast death. dimethyl sphingosine 129-149 sphingosine kinase 1 Homo sapiens 41-47 15993704-5 2005 EGF also activates sphingosine kinase-1 (SPHK-1), which converts sphingosine to sphingosine-1-phosphate, and its inhibition with dimethyl sphingosine (DMS) increased trophoblast death. dms 151-154 sphingosine kinase 1 Homo sapiens 19-39 15993704-5 2005 EGF also activates sphingosine kinase-1 (SPHK-1), which converts sphingosine to sphingosine-1-phosphate, and its inhibition with dimethyl sphingosine (DMS) increased trophoblast death. dms 151-154 sphingosine kinase 1 Homo sapiens 41-47 15693752-1 2005 Numerous extracellular stimuli activate SK1 (sphingosine kinase type 1) to catalyse the production of sphingosine 1-phosphate, a bioactive lipid that functions as both an extracellular ligand for a family of G-protein-linked receptors and as a putative intracellular messenger. sphingosine 1-phosphate 102-125 sphingosine kinase 1 Homo sapiens 40-43 15693752-1 2005 Numerous extracellular stimuli activate SK1 (sphingosine kinase type 1) to catalyse the production of sphingosine 1-phosphate, a bioactive lipid that functions as both an extracellular ligand for a family of G-protein-linked receptors and as a putative intracellular messenger. sphingosine 1-phosphate 102-125 sphingosine kinase 1 Homo sapiens 45-70 15693752-2 2005 Phorbol esters, calcium or immunoglobulin receptors stimulate SK1 by promoting its translocation to the plasma membrane, which brings it into proximity both to its substrate (i.e. sphingosine) and to activating acidic phospholipids (e.g. phosphatidylserine). Phorbol Esters 0-14 sphingosine kinase 1 Homo sapiens 62-65 15693752-2 2005 Phorbol esters, calcium or immunoglobulin receptors stimulate SK1 by promoting its translocation to the plasma membrane, which brings it into proximity both to its substrate (i.e. sphingosine) and to activating acidic phospholipids (e.g. phosphatidylserine). Calcium 16-23 sphingosine kinase 1 Homo sapiens 62-65 15693752-2 2005 Phorbol esters, calcium or immunoglobulin receptors stimulate SK1 by promoting its translocation to the plasma membrane, which brings it into proximity both to its substrate (i.e. sphingosine) and to activating acidic phospholipids (e.g. phosphatidylserine). Sphingosine 180-191 sphingosine kinase 1 Homo sapiens 62-65 15693752-2 2005 Phorbol esters, calcium or immunoglobulin receptors stimulate SK1 by promoting its translocation to the plasma membrane, which brings it into proximity both to its substrate (i.e. sphingosine) and to activating acidic phospholipids (e.g. phosphatidylserine). Phospholipids 218-231 sphingosine kinase 1 Homo sapiens 62-65 15693752-2 2005 Phorbol esters, calcium or immunoglobulin receptors stimulate SK1 by promoting its translocation to the plasma membrane, which brings it into proximity both to its substrate (i.e. sphingosine) and to activating acidic phospholipids (e.g. phosphatidylserine). Phosphatidylserines 238-256 sphingosine kinase 1 Homo sapiens 62-65 15848761-0 2005 Synthesis and evaluation of sphingoid analogs as inhibitors of sphingosine kinases. Sphingosine 28-37 sphingosine kinase 1 Homo sapiens 63-82 15848761-1 2005 Sphingosine 1-phosphate (S1P), a product of sphingosine kinases (SphK), mediates diverse biological processes such as cell differentiation, proliferation, motility, and apoptosis. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 44-63 15848761-4 2005 N,N-Dimethylsphingosine (DMS), a well-known SphK inhibitor, displayed inhibitory effects for both SphK1 and SphK2, as well as protein kinase C. In contrast, SG12 and SG14 exhibited selective inhibitory effects on hSphK2. N,N-dimethylsphingosine 25-28 sphingosine kinase 1 Homo sapiens 44-48 15848761-4 2005 N,N-Dimethylsphingosine (DMS), a well-known SphK inhibitor, displayed inhibitory effects for both SphK1 and SphK2, as well as protein kinase C. In contrast, SG12 and SG14 exhibited selective inhibitory effects on hSphK2. N,N-dimethylsphingosine 25-28 sphingosine kinase 1 Homo sapiens 98-103 15848761-1 2005 Sphingosine 1-phosphate (S1P), a product of sphingosine kinases (SphK), mediates diverse biological processes such as cell differentiation, proliferation, motility, and apoptosis. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 65-69 15848761-1 2005 Sphingosine 1-phosphate (S1P), a product of sphingosine kinases (SphK), mediates diverse biological processes such as cell differentiation, proliferation, motility, and apoptosis. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 44-63 15848761-1 2005 Sphingosine 1-phosphate (S1P), a product of sphingosine kinases (SphK), mediates diverse biological processes such as cell differentiation, proliferation, motility, and apoptosis. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 65-69 15848761-4 2005 N,N-Dimethylsphingosine (DMS), a well-known SphK inhibitor, displayed inhibitory effects for both SphK1 and SphK2, as well as protein kinase C. In contrast, SG12 and SG14 exhibited selective inhibitory effects on hSphK2. N,N-dimethylsphingosine 0-23 sphingosine kinase 1 Homo sapiens 44-48 15848761-4 2005 N,N-Dimethylsphingosine (DMS), a well-known SphK inhibitor, displayed inhibitory effects for both SphK1 and SphK2, as well as protein kinase C. In contrast, SG12 and SG14 exhibited selective inhibitory effects on hSphK2. N,N-dimethylsphingosine 0-23 sphingosine kinase 1 Homo sapiens 98-103 15863357-0 2005 Sphingosine kinase 1 is involved in dibutyryl cyclic AMP-induced granulocytic differentiation through the upregulation of extracellular signal-regulated kinase, but not p38 MAP kinase, in HL60 cells. dibutyryl 36-45 sphingosine kinase 1 Homo sapiens 0-20 15863357-1 2005 The role of sphingosine kinase (SPHK) in the dibutyryl cyclic AMP (dbcAMP)-induced granulocytic differentiation of HL60 cells was investigated. Bucladesine 45-65 sphingosine kinase 1 Homo sapiens 32-36 15863357-1 2005 The role of sphingosine kinase (SPHK) in the dibutyryl cyclic AMP (dbcAMP)-induced granulocytic differentiation of HL60 cells was investigated. Bucladesine 67-73 sphingosine kinase 1 Homo sapiens 12-30 15863357-1 2005 The role of sphingosine kinase (SPHK) in the dibutyryl cyclic AMP (dbcAMP)-induced granulocytic differentiation of HL60 cells was investigated. Bucladesine 67-73 sphingosine kinase 1 Homo sapiens 32-36 15863357-3 2005 Pretreatment of HL60 cells with N,N-dimethylsphingosine (DMS), a potent SPHK inhibitor, completely blocked dbcAMP-induced differentiation. N,N-dimethylsphingosine 32-55 sphingosine kinase 1 Homo sapiens 72-76 15863357-3 2005 Pretreatment of HL60 cells with N,N-dimethylsphingosine (DMS), a potent SPHK inhibitor, completely blocked dbcAMP-induced differentiation. N,N-dimethylsphingosine 57-60 sphingosine kinase 1 Homo sapiens 72-76 15863357-3 2005 Pretreatment of HL60 cells with N,N-dimethylsphingosine (DMS), a potent SPHK inhibitor, completely blocked dbcAMP-induced differentiation. Bucladesine 107-113 sphingosine kinase 1 Homo sapiens 72-76 15863357-5 2005 Pretreatment of HL60 cells with the MEK inhibitor, U0126, but not the p38 MAPK inhibitor, SB203580, completely suppressed dbcAMP-induced ERK1/2 activation and granulocytic differentiation, but did not affect the increase in SPHK activity. U 0126 51-56 sphingosine kinase 1 Homo sapiens 224-228 15743396-3 2005 Sphingosine kinase (SPHK) is the enzyme that phosphorylates sphingosine into sphingosine-1-phosphate (S1P), a potent bioactive sphingolipid. Sphingosine 60-71 sphingosine kinase 1 Homo sapiens 0-18 15743396-3 2005 Sphingosine kinase (SPHK) is the enzyme that phosphorylates sphingosine into sphingosine-1-phosphate (S1P), a potent bioactive sphingolipid. Sphingosine 60-71 sphingosine kinase 1 Homo sapiens 20-24 15743396-3 2005 Sphingosine kinase (SPHK) is the enzyme that phosphorylates sphingosine into sphingosine-1-phosphate (S1P), a potent bioactive sphingolipid. sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 0-18 15743396-3 2005 Sphingosine kinase (SPHK) is the enzyme that phosphorylates sphingosine into sphingosine-1-phosphate (S1P), a potent bioactive sphingolipid. sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 20-24 15743396-3 2005 Sphingosine kinase (SPHK) is the enzyme that phosphorylates sphingosine into sphingosine-1-phosphate (S1P), a potent bioactive sphingolipid. sphingosine 1-phosphate 102-105 sphingosine kinase 1 Homo sapiens 0-18 15743396-3 2005 Sphingosine kinase (SPHK) is the enzyme that phosphorylates sphingosine into sphingosine-1-phosphate (S1P), a potent bioactive sphingolipid. sphingosine 1-phosphate 102-105 sphingosine kinase 1 Homo sapiens 20-24 15743396-3 2005 Sphingosine kinase (SPHK) is the enzyme that phosphorylates sphingosine into sphingosine-1-phosphate (S1P), a potent bioactive sphingolipid. Sphingolipids 127-139 sphingosine kinase 1 Homo sapiens 0-18 15743396-3 2005 Sphingosine kinase (SPHK) is the enzyme that phosphorylates sphingosine into sphingosine-1-phosphate (S1P), a potent bioactive sphingolipid. Sphingolipids 127-139 sphingosine kinase 1 Homo sapiens 20-24 15743396-10 2005 The properties of SPHK activity in immune cells are linked to the functions of triggered growth and survival factors, phorbol esters, hormones, cytokines and chemokines, as well as antigen receptors, such as FcgammaRI and FcepsilonRI. Phorbol Esters 118-132 sphingosine kinase 1 Homo sapiens 18-22 15863357-0 2005 Sphingosine kinase 1 is involved in dibutyryl cyclic AMP-induced granulocytic differentiation through the upregulation of extracellular signal-regulated kinase, but not p38 MAP kinase, in HL60 cells. Cyclic AMP 46-56 sphingosine kinase 1 Homo sapiens 0-20 15863357-1 2005 The role of sphingosine kinase (SPHK) in the dibutyryl cyclic AMP (dbcAMP)-induced granulocytic differentiation of HL60 cells was investigated. Bucladesine 45-65 sphingosine kinase 1 Homo sapiens 12-30 15665242-2 2005 Recently, we have shown that the enzyme responsible for S1P production, sphingosine kinase (SphK), is stimulated by the phytohormone abscisic acid in guard cells of Arabidopsis (Arabidopsis thaliana) and that S1P is effective in regulating guard cell turgor. Abscisic Acid 133-146 sphingosine kinase 1 Homo sapiens 72-90 15715670-8 2005 Phorbol ester treatment resulted in an increase in sphingosine kinase 1 activity in the membranes, accompanied by a significant increase in extracellular S1P. Phorbol Esters 0-13 sphingosine kinase 1 Homo sapiens 51-71 15665242-2 2005 Recently, we have shown that the enzyme responsible for S1P production, sphingosine kinase (SphK), is stimulated by the phytohormone abscisic acid in guard cells of Arabidopsis (Arabidopsis thaliana) and that S1P is effective in regulating guard cell turgor. Abscisic Acid 133-146 sphingosine kinase 1 Homo sapiens 92-96 15665242-6 2005 In contrast, mammalian SphK1 efficiently phosphorylated Sph, dihydrosphingosine, and 4,8-sphingadienine, but not the 4-hydroxylated long-chain bases Phyto-Sph and 4-hydroxy-8-sphingenine. safingol 61-79 sphingosine kinase 1 Homo sapiens 23-28 15665242-6 2005 In contrast, mammalian SphK1 efficiently phosphorylated Sph, dihydrosphingosine, and 4,8-sphingadienine, but not the 4-hydroxylated long-chain bases Phyto-Sph and 4-hydroxy-8-sphingenine. sphinga-4,8-dienine 85-103 sphingosine kinase 1 Homo sapiens 23-28 15637591-4 2005 As sphingosine kinase (SphK) type 1 plays a critical role in determining the dynamic balance between the proapoptotic sphingolipid metabolite ceramide and the prosurvival S1P, we examined its role in apoptosis of melanoma cells. Sphingolipids 118-130 sphingosine kinase 1 Homo sapiens 3-21 15637591-0 2005 Sphingosine kinase activity counteracts ceramide-mediated cell death in human melanoma cells: role of Bcl-2 expression. Ceramides 40-48 sphingosine kinase 1 Homo sapiens 0-18 15637591-4 2005 As sphingosine kinase (SphK) type 1 plays a critical role in determining the dynamic balance between the proapoptotic sphingolipid metabolite ceramide and the prosurvival S1P, we examined its role in apoptosis of melanoma cells. Sphingolipids 118-130 sphingosine kinase 1 Homo sapiens 23-27 15637591-4 2005 As sphingosine kinase (SphK) type 1 plays a critical role in determining the dynamic balance between the proapoptotic sphingolipid metabolite ceramide and the prosurvival S1P, we examined its role in apoptosis of melanoma cells. Ceramides 142-150 sphingosine kinase 1 Homo sapiens 3-21 15637591-4 2005 As sphingosine kinase (SphK) type 1 plays a critical role in determining the dynamic balance between the proapoptotic sphingolipid metabolite ceramide and the prosurvival S1P, we examined its role in apoptosis of melanoma cells. Ceramides 142-150 sphingosine kinase 1 Homo sapiens 23-27 15637591-5 2005 Increasing SphK1 expression reduced the sensitivity of A-375 melanoma cells to Fas- and ceramide-mediated apoptosis. ammonium ferrous sulfate 79-82 sphingosine kinase 1 Homo sapiens 11-16 15637591-5 2005 Increasing SphK1 expression reduced the sensitivity of A-375 melanoma cells to Fas- and ceramide-mediated apoptosis. Ceramides 88-96 sphingosine kinase 1 Homo sapiens 11-16 15006402-0 2004 Fluorescence-labeled sphingosines as substrates of sphingosine kinases 1 and 2. Sphingosine 21-33 sphingosine kinase 1 Homo sapiens 51-78 15623571-1 2005 Sphingosine kinase (SK) 1 catalyzes the formation of the bioactive lipid sphingosine 1-phosphate, and has been implicated in several biological processes in mammalian cells, including enhanced proliferation, inhibition of apoptosis, and oncogenesis. sphingosine 1-phosphate 73-96 sphingosine kinase 1 Homo sapiens 0-25 17282113-1 2005 TNF&#945; stimulates SPHK in the monocyte, which leads to the expression of adhesion molecules on the cell surface. Adenosine Monophosphate 4-7 sphingosine kinase 1 Homo sapiens 25-29 17282113-3 2005 In this paper, we have delineated the TNF&#945;-induced and SPHK-dependent signaling pathway. Adenosine Monophosphate 42-45 sphingosine kinase 1 Homo sapiens 64-68 17282113-4 2005 In addition, we have developed a biomathematical model to qualify the SPHK time-dependent activity at a specific site in the cell upon TNF&#945; stimulation. Adenosine Monophosphate 139-142 sphingosine kinase 1 Homo sapiens 70-74 15733975-1 2005 We analyzed the intracellular action of sphingosine 1-phosphate (Sph-1-P), formed from sphingosine (Sph) by sphingosine kinase (SPHK), in platelets. sphingosine 1-phosphate 40-63 sphingosine kinase 1 Homo sapiens 108-126 15733975-1 2005 We analyzed the intracellular action of sphingosine 1-phosphate (Sph-1-P), formed from sphingosine (Sph) by sphingosine kinase (SPHK), in platelets. sphingosine 1-phosphate 40-63 sphingosine kinase 1 Homo sapiens 128-132 15733975-1 2005 We analyzed the intracellular action of sphingosine 1-phosphate (Sph-1-P), formed from sphingosine (Sph) by sphingosine kinase (SPHK), in platelets. sphingosine 1-phosphate 65-72 sphingosine kinase 1 Homo sapiens 108-126 15733975-1 2005 We analyzed the intracellular action of sphingosine 1-phosphate (Sph-1-P), formed from sphingosine (Sph) by sphingosine kinase (SPHK), in platelets. sphingosine 1-phosphate 65-72 sphingosine kinase 1 Homo sapiens 128-132 15733975-1 2005 We analyzed the intracellular action of sphingosine 1-phosphate (Sph-1-P), formed from sphingosine (Sph) by sphingosine kinase (SPHK), in platelets. Sphingosine 40-51 sphingosine kinase 1 Homo sapiens 108-126 15733975-1 2005 We analyzed the intracellular action of sphingosine 1-phosphate (Sph-1-P), formed from sphingosine (Sph) by sphingosine kinase (SPHK), in platelets. Sphingosine 40-51 sphingosine kinase 1 Homo sapiens 128-132 15733975-1 2005 We analyzed the intracellular action of sphingosine 1-phosphate (Sph-1-P), formed from sphingosine (Sph) by sphingosine kinase (SPHK), in platelets. Sphingosine 65-68 sphingosine kinase 1 Homo sapiens 108-126 15733975-1 2005 We analyzed the intracellular action of sphingosine 1-phosphate (Sph-1-P), formed from sphingosine (Sph) by sphingosine kinase (SPHK), in platelets. Sphingosine 65-68 sphingosine kinase 1 Homo sapiens 128-132 15733975-2 2005 When sphingosine kinase activity was inhibited by N,N-dimethylsphingosine (DMS), Ca2+ mobilization induced by convulxin, an agonist of the collagen receptor glycoprotein VI (GPVI), was moderately but specifically abolished; that induced via G protein-coupled receptors was not affected. N,N-dimethylsphingosine 50-73 sphingosine kinase 1 Homo sapiens 5-23 15733975-2 2005 When sphingosine kinase activity was inhibited by N,N-dimethylsphingosine (DMS), Ca2+ mobilization induced by convulxin, an agonist of the collagen receptor glycoprotein VI (GPVI), was moderately but specifically abolished; that induced via G protein-coupled receptors was not affected. N,N-dimethylsphingosine 75-78 sphingosine kinase 1 Homo sapiens 5-23 15302883-6 2004 Using the SPHK inhibitor N,N-dimethylsphingosine (DMS), we show that inhibition of SPHK abolishes the Ca2+ release from internal stores without inhibiting phospholipase C or protein kinase C activation triggered by C5a but has no effect on calcium signals triggered by other stimuli (FcgammaRII). N,N-dimethylsphingosine 25-48 sphingosine kinase 1 Homo sapiens 83-87 15302883-6 2004 Using the SPHK inhibitor N,N-dimethylsphingosine (DMS), we show that inhibition of SPHK abolishes the Ca2+ release from internal stores without inhibiting phospholipase C or protein kinase C activation triggered by C5a but has no effect on calcium signals triggered by other stimuli (FcgammaRII). N,N-dimethylsphingosine 50-53 sphingosine kinase 1 Homo sapiens 83-87 15302883-6 2004 Using the SPHK inhibitor N,N-dimethylsphingosine (DMS), we show that inhibition of SPHK abolishes the Ca2+ release from internal stores without inhibiting phospholipase C or protein kinase C activation triggered by C5a but has no effect on calcium signals triggered by other stimuli (FcgammaRII). Calcium 240-247 sphingosine kinase 1 Homo sapiens 83-87 15302883-8 2004 Moreover, an antisense oligonucleotide against SPHK1, in neutrophil-differentiated HL-60 cells, had similar inhibitory properties as DMS, suggesting that the SPHK utilized by C5a is SPHK1. Oligonucleotides 23-38 sphingosine kinase 1 Homo sapiens 47-52 15302883-8 2004 Moreover, an antisense oligonucleotide against SPHK1, in neutrophil-differentiated HL-60 cells, had similar inhibitory properties as DMS, suggesting that the SPHK utilized by C5a is SPHK1. Oligonucleotides 23-38 sphingosine kinase 1 Homo sapiens 47-51 15302883-8 2004 Moreover, an antisense oligonucleotide against SPHK1, in neutrophil-differentiated HL-60 cells, had similar inhibitory properties as DMS, suggesting that the SPHK utilized by C5a is SPHK1. Oligonucleotides 23-38 sphingosine kinase 1 Homo sapiens 182-187 15231724-5 2004 The tPA-induced sphingosine kinase-1 activation was mediated by Src, since it was inhibited by herbimycin A and in SrcK- cells (overexpressing a dominant negative kinase defective form of Src) and by ERK1/2 (early phase peaking at 15 min). herbimycin 95-107 sphingosine kinase 1 Homo sapiens 16-36 15231724-6 2004 Sphingosine kinase-1 activation was followed by a second phase of ERK1/2 phosphorylation (peaking at 120 min) and subsequent DNA synthesis, which were inhibited by D-MAPP and DMS, by anti-EGD-1 antibodies and in SrcK- cells (in which the mitogenic signaling was rescued by sphingosine-1-phosphate). d-mapp 164-170 sphingosine kinase 1 Homo sapiens 0-20 15231724-6 2004 Sphingosine kinase-1 activation was followed by a second phase of ERK1/2 phosphorylation (peaking at 120 min) and subsequent DNA synthesis, which were inhibited by D-MAPP and DMS, by anti-EGD-1 antibodies and in SrcK- cells (in which the mitogenic signaling was rescued by sphingosine-1-phosphate). N,N-dimethylsphingosine 175-178 sphingosine kinase 1 Homo sapiens 0-20 15231724-6 2004 Sphingosine kinase-1 activation was followed by a second phase of ERK1/2 phosphorylation (peaking at 120 min) and subsequent DNA synthesis, which were inhibited by D-MAPP and DMS, by anti-EGD-1 antibodies and in SrcK- cells (in which the mitogenic signaling was rescued by sphingosine-1-phosphate). sphingosine 1-phosphate 273-296 sphingosine kinase 1 Homo sapiens 0-20 15246004-3 2004 We have shown that both human sphingosine kinase 1 and 2 (hSK1 and hSK2) can efficiently phosphorylate biotinyl-Sph, with K(m) values similar to those of sphingosine. biotinyl-sph 103-115 sphingosine kinase 1 Homo sapiens 30-56 15246004-3 2004 We have shown that both human sphingosine kinase 1 and 2 (hSK1 and hSK2) can efficiently phosphorylate biotinyl-Sph, with K(m) values similar to those of sphingosine. biotinyl-sph 103-115 sphingosine kinase 1 Homo sapiens 58-62 15246004-3 2004 We have shown that both human sphingosine kinase 1 and 2 (hSK1 and hSK2) can efficiently phosphorylate biotinyl-Sph, with K(m) values similar to those of sphingosine. Sphingosine 30-41 sphingosine kinase 1 Homo sapiens 58-62 15196915-1 2004 Sphingosine kinase type 1 (SphK1) and its product sphingosine-1-phosphate have been shown to promote cell growth and inhibit apoptosis of tumor cells. sphingosine 1-phosphate 50-73 sphingosine kinase 1 Homo sapiens 0-25 15196915-1 2004 Sphingosine kinase type 1 (SphK1) and its product sphingosine-1-phosphate have been shown to promote cell growth and inhibit apoptosis of tumor cells. sphingosine 1-phosphate 50-73 sphingosine kinase 1 Homo sapiens 27-32 14988393-1 2004 Sphingosine kinase 1 (SK1), a key enzyme in sphingosine 1-phosphate (S1P) synthesis, regulates various aspects of cell behavior, including cell survival and proliferation. sphingosine 1-phosphate 44-67 sphingosine kinase 1 Homo sapiens 0-20 14988393-1 2004 Sphingosine kinase 1 (SK1), a key enzyme in sphingosine 1-phosphate (S1P) synthesis, regulates various aspects of cell behavior, including cell survival and proliferation. sphingosine 1-phosphate 44-67 sphingosine kinase 1 Homo sapiens 22-25 14988393-1 2004 Sphingosine kinase 1 (SK1), a key enzyme in sphingosine 1-phosphate (S1P) synthesis, regulates various aspects of cell behavior, including cell survival and proliferation. sphingosine 1-phosphate 69-72 sphingosine kinase 1 Homo sapiens 0-20 14988393-1 2004 Sphingosine kinase 1 (SK1), a key enzyme in sphingosine 1-phosphate (S1P) synthesis, regulates various aspects of cell behavior, including cell survival and proliferation. sphingosine 1-phosphate 69-72 sphingosine kinase 1 Homo sapiens 22-25 14988393-3 2004 In this study, we investigated the effects of a DNA damaging agent, actinomycin D (Act D), on the function of sphingosine kinase (SK1). Dactinomycin 68-81 sphingosine kinase 1 Homo sapiens 130-133 14988393-6 2004 Similar decreases in SK1 protein were observed with other DNA damaging agents such as doxorubicin, etoposide, and gamma-irradiation. Doxorubicin 86-97 sphingosine kinase 1 Homo sapiens 21-24 14988393-6 2004 Similar decreases in SK1 protein were observed with other DNA damaging agents such as doxorubicin, etoposide, and gamma-irradiation. Etoposide 99-108 sphingosine kinase 1 Homo sapiens 21-24 14988393-7 2004 ZVAD, the pancaspase inhibitor, and Bcl-2 annulled the effect of Act D on SK1, demonstrating a role for cysteine proteases downstream of Bcl-2 in the down-regulation of SK1. benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone 0-4 sphingosine kinase 1 Homo sapiens 74-77 14988393-7 2004 ZVAD, the pancaspase inhibitor, and Bcl-2 annulled the effect of Act D on SK1, demonstrating a role for cysteine proteases downstream of Bcl-2 in the down-regulation of SK1. benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone 0-4 sphingosine kinase 1 Homo sapiens 169-172 14984734-1 2004 Sphingosine 1-phosphate (S1P) is a bioactive lipid mediator generated from sphingosine by sphingosine kinase (SPHK). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 90-108 14984734-1 2004 Sphingosine 1-phosphate (S1P) is a bioactive lipid mediator generated from sphingosine by sphingosine kinase (SPHK). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 110-114 14984734-1 2004 Sphingosine 1-phosphate (S1P) is a bioactive lipid mediator generated from sphingosine by sphingosine kinase (SPHK). Sphingosine 75-86 sphingosine kinase 1 Homo sapiens 90-108 14984734-1 2004 Sphingosine 1-phosphate (S1P) is a bioactive lipid mediator generated from sphingosine by sphingosine kinase (SPHK). Sphingosine 75-86 sphingosine kinase 1 Homo sapiens 110-114 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 29-60 sphingosine kinase 1 Homo sapiens 143-161 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 29-60 sphingosine kinase 1 Homo sapiens 163-167 15310762-0 2004 Sphingosine kinase 1 is an intracellular effector of phosphatidic acid. Phosphatidic Acids 53-70 sphingosine kinase 1 Homo sapiens 0-20 15310762-1 2004 Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Sphingosine 42-53 sphingosine kinase 1 Homo sapiens 0-20 15310762-1 2004 Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Sphingosine 42-53 sphingosine kinase 1 Homo sapiens 22-25 15310762-1 2004 Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 66-89 sphingosine kinase 1 Homo sapiens 0-20 15310762-1 2004 Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 66-89 sphingosine kinase 1 Homo sapiens 22-25 15310762-3 2004 We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. Phosphatidic Acids 131-148 sphingosine kinase 1 Homo sapiens 15-18 15310762-3 2004 We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. Phosphatidic Acids 131-148 sphingosine kinase 1 Homo sapiens 245-248 15310762-3 2004 We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. Phosphatidic Acids 150-152 sphingosine kinase 1 Homo sapiens 15-18 15310762-3 2004 We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. Phosphatidic Acids 150-152 sphingosine kinase 1 Homo sapiens 245-248 15310762-3 2004 We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. Phosphatidic Acids 254-256 sphingosine kinase 1 Homo sapiens 15-18 15310762-3 2004 We report that SK1, which is ordinarily a cytosolic enzyme, translocates in vivo and in vitro to membrane compartments enriched in phosphatidic acid (PA), the lipid product of phospholipase D. This translocation depends on direct interaction of SK1 with PA, because recombinant purified enzyme shows strong affinity for pure PA coupled to Affi-Gel. Phosphatidic Acids 254-256 sphingosine kinase 1 Homo sapiens 15-18 15310762-5 2004 Thus SK1 constitutes a novel, physiologically relevant PA effector. Phosphatidic Acids 55-57 sphingosine kinase 1 Homo sapiens 5-8 15265887-6 2004 Using an antisense oligonucleotide against SPHK1, we show that knockdown of SPHK1 abolishes the C5a-triggered intracellular Ca(2+) signals, degranulation, cytokine generation, and chemotaxis. Oligonucleotides 19-34 sphingosine kinase 1 Homo sapiens 76-81 14988393-9 2004 Inhibition of cathepsin B, a lysosomal protease, produced a significant reversal of SK1 decline by Act D, suggesting that a multitude of ZVAD-sensitive cysteine proteases downstream of Bcl-2 mediated the SK1 decrease. benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone 137-141 sphingosine kinase 1 Homo sapiens 84-87 14988393-9 2004 Inhibition of cathepsin B, a lysosomal protease, produced a significant reversal of SK1 decline by Act D, suggesting that a multitude of ZVAD-sensitive cysteine proteases downstream of Bcl-2 mediated the SK1 decrease. benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone 137-141 sphingosine kinase 1 Homo sapiens 204-207 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 29-60 sphingosine kinase 1 Homo sapiens 189-194 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 29-60 sphingosine kinase 1 Homo sapiens 225-230 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 62-65 sphingosine kinase 1 Homo sapiens 143-161 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 62-65 sphingosine kinase 1 Homo sapiens 163-167 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 62-65 sphingosine kinase 1 Homo sapiens 189-194 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. Tetradecanoylphorbol Acetate 62-65 sphingosine kinase 1 Homo sapiens 225-230 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. meg-o1 115-121 sphingosine kinase 1 Homo sapiens 143-161 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. meg-o1 115-121 sphingosine kinase 1 Homo sapiens 163-167 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. meg-o1 115-121 sphingosine kinase 1 Homo sapiens 189-194 14729073-1 2003 The prolonged treatment with phorbol 12-myristate 13-acetate (PMA) of a human megakaryoblastic leukemia cell line, MEG-O1, induced increase of sphingosine kinase (SPHK) enzyme activity and SPHK1 protein expression as well as SPHK1 message. meg-o1 115-121 sphingosine kinase 1 Homo sapiens 225-230 14729073-2 2003 Protein kinase C (PKC) inhibitor prevented the PMA-induced SPHK1 gene expression. Tetradecanoylphorbol Acetate 47-50 sphingosine kinase 1 Homo sapiens 59-64 15006402-2 2004 Determining the influence of the nature, position and linkage of the label on the in vitro phosphorylation rate by sphingosine kinases 1 and 2 resulted in the identification of a pyrene- and a NBD-labeled sphingosine which are both phosphorylated with efficiency comparable to the natural substrate. pyrene 179-185 sphingosine kinase 1 Homo sapiens 115-142 12881510-7 2003 In contrast, expression of SphKG82D, a dominant-negative mutant SphK, profoundly inhibited the E2-mediated Ca2+ mobilization, Erk1/2 activity and neoplastic cell growth. Estradiol 95-97 sphingosine kinase 1 Homo sapiens 27-31 12954646-1 2003 Sphingosine kinase-1 (SPHK1) is a key enzyme catalyzing the formation of an important bioactive lipid messenger, sphingosine 1-phosphate, and is implicated in the regulation of cell proliferation and antiapoptotic processes. sphingosine 1-phosphate 113-136 sphingosine kinase 1 Homo sapiens 0-20 12954646-1 2003 Sphingosine kinase-1 (SPHK1) is a key enzyme catalyzing the formation of an important bioactive lipid messenger, sphingosine 1-phosphate, and is implicated in the regulation of cell proliferation and antiapoptotic processes. sphingosine 1-phosphate 113-136 sphingosine kinase 1 Homo sapiens 22-27 14575709-1 2003 Sphingosine kinase (SPHK) is an enzyme that phosphorylates sphingosine to form sphingosine 1-phosphate (S1P). Sphingosine 59-70 sphingosine kinase 1 Homo sapiens 0-18 14575709-1 2003 Sphingosine kinase (SPHK) is an enzyme that phosphorylates sphingosine to form sphingosine 1-phosphate (S1P). Sphingosine 59-70 sphingosine kinase 1 Homo sapiens 20-24 14575709-1 2003 Sphingosine kinase (SPHK) is an enzyme that phosphorylates sphingosine to form sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 79-102 sphingosine kinase 1 Homo sapiens 0-18 14575709-1 2003 Sphingosine kinase (SPHK) is an enzyme that phosphorylates sphingosine to form sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 79-102 sphingosine kinase 1 Homo sapiens 20-24 14575709-5 2003 Furthermore, upon treatment with leptomycin B, a specific inhibitor of the nuclear export receptor CRM1, a marked nuclear accumulation of hSPHK1 was observed, indicating that hSPHK1 shuttles between the cytoplasm and the nucleus. leptomycin B 33-45 sphingosine kinase 1 Homo sapiens 138-144 14575709-5 2003 Furthermore, upon treatment with leptomycin B, a specific inhibitor of the nuclear export receptor CRM1, a marked nuclear accumulation of hSPHK1 was observed, indicating that hSPHK1 shuttles between the cytoplasm and the nucleus. leptomycin B 33-45 sphingosine kinase 1 Homo sapiens 175-181 12835323-1 2003 There are two isoforms of sphingosine kinase (SphK) that catalyze the formation of sphingosine 1-phosphate, a potent sphingolipid mediator. sphingosine 1-phosphate 83-106 sphingosine kinase 1 Homo sapiens 26-44 12835323-1 2003 There are two isoforms of sphingosine kinase (SphK) that catalyze the formation of sphingosine 1-phosphate, a potent sphingolipid mediator. sphingosine 1-phosphate 83-106 sphingosine kinase 1 Homo sapiens 46-50 12835323-1 2003 There are two isoforms of sphingosine kinase (SphK) that catalyze the formation of sphingosine 1-phosphate, a potent sphingolipid mediator. Sphingolipids 117-129 sphingosine kinase 1 Homo sapiens 26-44 12835323-1 2003 There are two isoforms of sphingosine kinase (SphK) that catalyze the formation of sphingosine 1-phosphate, a potent sphingolipid mediator. Sphingolipids 117-129 sphingosine kinase 1 Homo sapiens 46-50 12881510-2 2003 Here we report that treatment with 17beta-estradiol (E2) activates a novel signaling pathway via activation of sphingosine kinase (SphK) in MCF-7 breast cancer cells. Estradiol 35-51 sphingosine kinase 1 Homo sapiens 111-129 12881510-2 2003 Here we report that treatment with 17beta-estradiol (E2) activates a novel signaling pathway via activation of sphingosine kinase (SphK) in MCF-7 breast cancer cells. Estradiol 35-51 sphingosine kinase 1 Homo sapiens 131-135 12881510-2 2003 Here we report that treatment with 17beta-estradiol (E2) activates a novel signaling pathway via activation of sphingosine kinase (SphK) in MCF-7 breast cancer cells. Estradiol 53-55 sphingosine kinase 1 Homo sapiens 111-129 13129923-6 2003 Further SPHK superfamily members, namely ceramide kinase and a "SPHK-like" protein, failed to phosphorylate sphingosine and FTY720. Sphingosine 108-119 sphingosine kinase 1 Homo sapiens 8-12 13129923-6 2003 Further SPHK superfamily members, namely ceramide kinase and a "SPHK-like" protein, failed to phosphorylate sphingosine and FTY720. Fingolimod Hydrochloride 124-130 sphingosine kinase 1 Homo sapiens 8-12 13129923-10 2003 In human endothelial cells, while activity of SPHK1 toward sphingosine was 2-fold higher than of SPHK2, FTY720 phosphorylation was 7-fold faster under SPHK2 assay conditions. Sphingosine 59-70 sphingosine kinase 1 Homo sapiens 46-51 14532121-1 2003 Sphingosine kinase 1 is an agonist-activated signalling enzyme that catalyses the formation of sphingosine 1-phosphate, a lipid second messenger that has been implicated in a number of agonist-driven cellular responses, including stimulation of cell proliferation, inhibition of apoptosis and expression of inflammatory molecules. sphingosine 1-phosphate 95-118 sphingosine kinase 1 Homo sapiens 0-20 12881510-2 2003 Here we report that treatment with 17beta-estradiol (E2) activates a novel signaling pathway via activation of sphingosine kinase (SphK) in MCF-7 breast cancer cells. Estradiol 53-55 sphingosine kinase 1 Homo sapiens 131-135 12847068-2 2003 METHODS AND RESULTS: We tested whether sphingosine kinase (Sphk1) that generates the endogenous sphingolipid mediator sphingosine-1-phosphate (S1P) is part of a signaling cascade to activate the RhoA/Rho kinase pathway. Sphingolipids 96-108 sphingosine kinase 1 Homo sapiens 59-64 12847068-2 2003 METHODS AND RESULTS: We tested whether sphingosine kinase (Sphk1) that generates the endogenous sphingolipid mediator sphingosine-1-phosphate (S1P) is part of a signaling cascade to activate the RhoA/Rho kinase pathway. sphingosine 1-phosphate 118-141 sphingosine kinase 1 Homo sapiens 59-64 12847068-2 2003 METHODS AND RESULTS: We tested whether sphingosine kinase (Sphk1) that generates the endogenous sphingolipid mediator sphingosine-1-phosphate (S1P) is part of a signaling cascade to activate the RhoA/Rho kinase pathway. sphingosine 1-phosphate 143-146 sphingosine kinase 1 Homo sapiens 59-64 12531188-1 2003 Activation of sphingosine kinase (SPHK), thereby increasing cellular levels of sphingosine 1-phosphate (S1P), may be involved in a variety of intracellular responses including Ca(2+) signaling. sphingosine 1-phosphate 79-102 sphingosine kinase 1 Homo sapiens 14-32 12789341-4 2003 Consistent with this observation, inhibition of SphK attenuates ABA regulation of guard cell inward K(+) channels and slow anion channels, which are involved in the regulation of stomatal pore size. Abscisic Acid 64-67 sphingosine kinase 1 Homo sapiens 48-52 12776187-9 2003 We present a model where the balance between sphingosine and SPP regulates Ras-GAP activity such that stimulation of SPK1 favors downregulation of Ras-GAP and thereby the activation of Ras proteins. Sphingosine 45-56 sphingosine kinase 1 Homo sapiens 117-121 12531188-1 2003 Activation of sphingosine kinase (SPHK), thereby increasing cellular levels of sphingosine 1-phosphate (S1P), may be involved in a variety of intracellular responses including Ca(2+) signaling. sphingosine 1-phosphate 79-102 sphingosine kinase 1 Homo sapiens 34-38 12531188-1 2003 Activation of sphingosine kinase (SPHK), thereby increasing cellular levels of sphingosine 1-phosphate (S1P), may be involved in a variety of intracellular responses including Ca(2+) signaling. sphingosine 1-phosphate 104-107 sphingosine kinase 1 Homo sapiens 14-32 12531188-1 2003 Activation of sphingosine kinase (SPHK), thereby increasing cellular levels of sphingosine 1-phosphate (S1P), may be involved in a variety of intracellular responses including Ca(2+) signaling. sphingosine 1-phosphate 104-107 sphingosine kinase 1 Homo sapiens 34-38 12485162-1 2002 BACKGROUND: The bioactive sphingolipid sphingosine 1-phosphate (S1P) is formed by the activation of sphingosine kinase (SPHK) in diverse stimuli, such as platelet-derived growth factor (PDGF). Sphingolipids 26-38 sphingosine kinase 1 Homo sapiens 100-118 12531554-5 2002 A central finding of these studies is that Sph kinase (SphK), the enzyme that phosphorylates Sph to form S1P, is a critical regulator of the sphingolipid rheostat, as it not only produces the pro-growth, anti-apoptotic messenger S1P, but also decreases levels of pro-apoptotic Cer and Sph. Sphingolipids 141-153 sphingosine kinase 1 Homo sapiens 43-53 12531554-5 2002 A central finding of these studies is that Sph kinase (SphK), the enzyme that phosphorylates Sph to form S1P, is a critical regulator of the sphingolipid rheostat, as it not only produces the pro-growth, anti-apoptotic messenger S1P, but also decreases levels of pro-apoptotic Cer and Sph. Sphingolipids 141-153 sphingosine kinase 1 Homo sapiens 55-59 12393916-4 2002 Here we have elucidated the nucleotide-binding site of human sphingosine kinase 1 (hSK1) through a combination of site-directed mutagenesis and affinity labeling with the ATP analogue, FSBA. Adenosine Triphosphate 171-174 sphingosine kinase 1 Homo sapiens 61-81 12393916-4 2002 Here we have elucidated the nucleotide-binding site of human sphingosine kinase 1 (hSK1) through a combination of site-directed mutagenesis and affinity labeling with the ATP analogue, FSBA. Adenosine Triphosphate 171-174 sphingosine kinase 1 Homo sapiens 83-87 12393916-5 2002 We have shown that Gly(82) of hSK1 is involved in ATP binding since mutation of this residue to alanine resulted in an enzyme with an approximately 45-fold higher K(m)((ATP)). Glycine 19-22 sphingosine kinase 1 Homo sapiens 30-34 12393916-5 2002 We have shown that Gly(82) of hSK1 is involved in ATP binding since mutation of this residue to alanine resulted in an enzyme with an approximately 45-fold higher K(m)((ATP)). Adenosine Triphosphate 50-53 sphingosine kinase 1 Homo sapiens 30-34 12393916-5 2002 We have shown that Gly(82) of hSK1 is involved in ATP binding since mutation of this residue to alanine resulted in an enzyme with an approximately 45-fold higher K(m)((ATP)). Alanine 96-103 sphingosine kinase 1 Homo sapiens 30-34 12393916-5 2002 We have shown that Gly(82) of hSK1 is involved in ATP binding since mutation of this residue to alanine resulted in an enzyme with an approximately 45-fold higher K(m)((ATP)). Adenosine Triphosphate 169-172 sphingosine kinase 1 Homo sapiens 30-34 12485162-1 2002 BACKGROUND: The bioactive sphingolipid sphingosine 1-phosphate (S1P) is formed by the activation of sphingosine kinase (SPHK) in diverse stimuli, such as platelet-derived growth factor (PDGF). Sphingolipids 26-38 sphingosine kinase 1 Homo sapiens 120-124 12485162-1 2002 BACKGROUND: The bioactive sphingolipid sphingosine 1-phosphate (S1P) is formed by the activation of sphingosine kinase (SPHK) in diverse stimuli, such as platelet-derived growth factor (PDGF). sphingosine 1-phosphate 39-62 sphingosine kinase 1 Homo sapiens 100-118 12485162-1 2002 BACKGROUND: The bioactive sphingolipid sphingosine 1-phosphate (S1P) is formed by the activation of sphingosine kinase (SPHK) in diverse stimuli, such as platelet-derived growth factor (PDGF). sphingosine 1-phosphate 39-62 sphingosine kinase 1 Homo sapiens 120-124 12485162-1 2002 BACKGROUND: The bioactive sphingolipid sphingosine 1-phosphate (S1P) is formed by the activation of sphingosine kinase (SPHK) in diverse stimuli, such as platelet-derived growth factor (PDGF). sphingosine 1-phosphate 64-67 sphingosine kinase 1 Homo sapiens 100-118 12485162-1 2002 BACKGROUND: The bioactive sphingolipid sphingosine 1-phosphate (S1P) is formed by the activation of sphingosine kinase (SPHK) in diverse stimuli, such as platelet-derived growth factor (PDGF). sphingosine 1-phosphate 64-67 sphingosine kinase 1 Homo sapiens 120-124 12485162-8 2002 N,N-dimethylsphingosine, a competitive inhibitor of SPHK, reduced PDGF-induced mesangial cell proliferation, whereas over-expression of SPHK promoted it. N,N-dimethylsphingosine 0-23 sphingosine kinase 1 Homo sapiens 52-56 12391145-6 2002 Small interfering RNA (siRNA) that targets SPK1, but not SPK2, blocks VEGF-induced accumulation of Ras-GTP and phospho-ERK in T24 cells. ras-gtp 99-106 sphingosine kinase 1 Homo sapiens 43-47 12441135-3 2002 Enforced expression of sphingosine kinase type 1 (SPHK1) increased S1P levels and blocked MCF-7 cell death induced by anti-cancer drugs, sphingosine, and TNF-alpha. Sphingosine 23-34 sphingosine kinase 1 Homo sapiens 50-55 12204793-1 2002 The role of sphingosine kinase (SphK) on basic fibroblast growth factor (bFGF)-induced proliferation of cerebral, aortic and coronary smooth muscle cells (SMC) was addressed using D-erythro-N,N-dimethylsphingosine (DMS), an inhibitor of SphK which blocks conversion of sphingosine to sphingosine-1-phosphate (S1P). Sphingosine 12-23 sphingosine kinase 1 Homo sapiens 32-36 12080051-3 2002 Growth factors rapidly activate sphingosine kinase type 1 (SPHK1) resulting in phosphorylation of sphingosine to form S1P, which plays important roles in cell growth regulation and protection from apoptosis. Sphingosine 32-43 sphingosine kinase 1 Homo sapiens 59-64 12102559-1 2002 Sphingosine kinase (SPHK) catalyzes the formation of sphingosine-1-phosphate (S11). sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 0-18 12124383-7 2002 PMA induced translocation of both endogenous and green fluorescent protein (GFP)-tagged human SK1 (hSK1) to the plasma membrane. Tetradecanoylphorbol Acetate 0-3 sphingosine kinase 1 Homo sapiens 94-97 12124383-7 2002 PMA induced translocation of both endogenous and green fluorescent protein (GFP)-tagged human SK1 (hSK1) to the plasma membrane. Tetradecanoylphorbol Acetate 0-3 sphingosine kinase 1 Homo sapiens 99-103 12124383-8 2002 PMA also induced phosphorylation of GFP-hSK1. Tetradecanoylphorbol Acetate 0-3 sphingosine kinase 1 Homo sapiens 40-44 11777919-3 2002 We now report a novel mechanism of TRAF2-mediated signal transduction revealed by an association of TRAF2 with sphingosine kinase (SphK), a lipid kinase that is responsible for the production of sphingosine 1-phosphate. sphingosine 1-phosphate 195-218 sphingosine kinase 1 Homo sapiens 111-129 11777919-3 2002 We now report a novel mechanism of TRAF2-mediated signal transduction revealed by an association of TRAF2 with sphingosine kinase (SphK), a lipid kinase that is responsible for the production of sphingosine 1-phosphate. sphingosine 1-phosphate 195-218 sphingosine kinase 1 Homo sapiens 131-135 11741921-8 2002 The export of SK-1 is constitutive and is inhibited by cytochalasin D and treatment at 4 degrees C but not by brefeldin A or nocodazole, suggesting that a nonclassical secretory pathway that requires the actin cytoskeleton dynamics is involved. Cytochalasin D 55-69 sphingosine kinase 1 Homo sapiens 14-18 11741921-8 2002 The export of SK-1 is constitutive and is inhibited by cytochalasin D and treatment at 4 degrees C but not by brefeldin A or nocodazole, suggesting that a nonclassical secretory pathway that requires the actin cytoskeleton dynamics is involved. Brefeldin A 110-121 sphingosine kinase 1 Homo sapiens 14-18 11741582-3 2001 Here we have tested the functional importance of Gly(113), a highly conserved residue of human sphingosine kinase 1 (hSK), by site-directed mutagenesis. Glycine 49-52 sphingosine kinase 1 Homo sapiens 95-115 12102559-1 2002 Sphingosine kinase (SPHK) catalyzes the formation of sphingosine-1-phosphate (S11). sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 20-24 12102559-1 2002 Sphingosine kinase (SPHK) catalyzes the formation of sphingosine-1-phosphate (S11). capravirine 78-81 sphingosine kinase 1 Homo sapiens 0-18 12102559-1 2002 Sphingosine kinase (SPHK) catalyzes the formation of sphingosine-1-phosphate (S11). capravirine 78-81 sphingosine kinase 1 Homo sapiens 20-24 11418646-5 2001 Pretreatment of cells with N,N-dimethylsphingosine (DMS), an inhibitor of SphK, or LY 294002, an inhibitor of PI3K that acts upstream of Akt, increased the number of apoptotic cells induced by TNF-alpha in Ad5IkappaB-infected Huh-7 and Hc cells. N,N-dimethylsphingosine 27-50 sphingosine kinase 1 Homo sapiens 74-78 11520048-1 2001 In hepatoma Huh-7 cells, inhibition of sphingosine kinase (SphK) activity by N,N-dimethylsphingosine (DMS) resulted in up-regulated production of liver-specific serum proteins including albumin and alpha-fetoprotein (AFP). N,N-dimethylsphingosine 77-100 sphingosine kinase 1 Homo sapiens 39-57 11520048-1 2001 In hepatoma Huh-7 cells, inhibition of sphingosine kinase (SphK) activity by N,N-dimethylsphingosine (DMS) resulted in up-regulated production of liver-specific serum proteins including albumin and alpha-fetoprotein (AFP). N,N-dimethylsphingosine 77-100 sphingosine kinase 1 Homo sapiens 59-63 11520048-1 2001 In hepatoma Huh-7 cells, inhibition of sphingosine kinase (SphK) activity by N,N-dimethylsphingosine (DMS) resulted in up-regulated production of liver-specific serum proteins including albumin and alpha-fetoprotein (AFP). N,N-dimethylsphingosine 102-105 sphingosine kinase 1 Homo sapiens 39-57 11520048-1 2001 In hepatoma Huh-7 cells, inhibition of sphingosine kinase (SphK) activity by N,N-dimethylsphingosine (DMS) resulted in up-regulated production of liver-specific serum proteins including albumin and alpha-fetoprotein (AFP). N,N-dimethylsphingosine 102-105 sphingosine kinase 1 Homo sapiens 59-63 11520048-5 2001 These results suggest that activation of SphK disengages cells from their liver-specific phenotype, and that 9-cis RA further induces differentiation of hepatoma cells when SphK activity is inhibited. Tretinoin 115-117 sphingosine kinase 1 Homo sapiens 173-177 11418646-5 2001 Pretreatment of cells with N,N-dimethylsphingosine (DMS), an inhibitor of SphK, or LY 294002, an inhibitor of PI3K that acts upstream of Akt, increased the number of apoptotic cells induced by TNF-alpha in Ad5IkappaB-infected Huh-7 and Hc cells. N,N-dimethylsphingosine 52-55 sphingosine kinase 1 Homo sapiens 74-78 11418646-7 2001 In contrast, exogenous sphingosine 1-phosphate, a product of SphK, was found to activate Akt and partially rescued the cells from TNF-alpha-induced apoptosis. sphingosine 1-phosphate 23-46 sphingosine kinase 1 Homo sapiens 61-65 10751414-11 2000 Triton X-100 inhibited SPHK2 and stimulated SPHK1, whereas phosphatidylserine stimulated both type 1 and type 2 SPHK. Octoxynol 0-12 sphingosine kinase 1 Homo sapiens 44-49 10961989-9 2000 In the light of previous studies, these data provide evidence that SK1-59 contributes to fibrin-independent plasminogen activation through 1) intermolecular interactions with the plasmin in the activator complex, 2) binding interactions with the plasminogen substrate, and 3) intramolecular interactions that structure the A domain of SK for Pg substrate processing. pg 342-344 sphingosine kinase 1 Homo sapiens 67-70 1899230-5 1991 The spk1+ gene product is a 45-kD protein enriched in the nucleus, and its level increases 10-fold after addition of staurosporine. Staurosporine 117-130 sphingosine kinase 1 Homo sapiens 4-8 10567432-2 1999 Here we report that TNF activates another key enzyme, sphingosine kinase (SphK), in the sphingomyelin metabolic pathway resulting in production of sphingosine-1-phosphate (S1P) and that S1P is a potent antagonist of TNF-mediated apoptosis. Sphingomyelins 88-101 sphingosine kinase 1 Homo sapiens 54-72 10567432-2 1999 Here we report that TNF activates another key enzyme, sphingosine kinase (SphK), in the sphingomyelin metabolic pathway resulting in production of sphingosine-1-phosphate (S1P) and that S1P is a potent antagonist of TNF-mediated apoptosis. Sphingomyelins 88-101 sphingosine kinase 1 Homo sapiens 74-78 10567432-2 1999 Here we report that TNF activates another key enzyme, sphingosine kinase (SphK), in the sphingomyelin metabolic pathway resulting in production of sphingosine-1-phosphate (S1P) and that S1P is a potent antagonist of TNF-mediated apoptosis. sphingosine 1-phosphate 147-170 sphingosine kinase 1 Homo sapiens 54-72 10567432-2 1999 Here we report that TNF activates another key enzyme, sphingosine kinase (SphK), in the sphingomyelin metabolic pathway resulting in production of sphingosine-1-phosphate (S1P) and that S1P is a potent antagonist of TNF-mediated apoptosis. sphingosine 1-phosphate 147-170 sphingosine kinase 1 Homo sapiens 74-78 10567432-3 1999 The TNF-induced SphK activation is independent of sphingomyelinase and ceramidase activities, suggesting that TNF affects this enzyme directly other than through a mass effect on sphingomyelin degradation. Sphingomyelins 50-63 sphingosine kinase 1 Homo sapiens 16-20 10567432-6 1999 Conversely, N, N-dimethylsphingosine, an inhibitor of SphK, profoundly sensitized normal HUVEC to killing by TNF. N,N-dimethylsphingosine 12-36 sphingosine kinase 1 Homo sapiens 54-58 10802064-5 2000 hSPHK1 also specifically phosphorylated D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Sphingosine 40-61 sphingosine kinase 1 Homo sapiens 0-6 10802064-5 2000 hSPHK1 also specifically phosphorylated D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. safingol 85-96 sphingosine kinase 1 Homo sapiens 0-6 10802064-5 2000 hSPHK1 also specifically phosphorylated D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. N,N-dimethylsphingosine 158-182 sphingosine kinase 1 Homo sapiens 0-6 10802064-5 2000 hSPHK1 also specifically phosphorylated D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Diglycerides 184-198 sphingosine kinase 1 Homo sapiens 0-6 10802064-5 2000 hSPHK1 also specifically phosphorylated D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Ceramides 200-208 sphingosine kinase 1 Homo sapiens 0-6 10802064-5 2000 hSPHK1 also specifically phosphorylated D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N, N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Phosphatidylinositols 213-233 sphingosine kinase 1 Homo sapiens 0-6 1899230-7 1991 The pap1+ gene is required for spk1(+)-conferred staurosporine resistance. Staurosporine 49-62 sphingosine kinase 1 Homo sapiens 31-35 15923363-1 2005 Sphingosine kinase 1 (SK1) is a key enzyme critical to the sphingolipid metabolic pathway responsible for catalyzing the formation of the bioactive lipid sphingosine-1-phosphate. Sphingolipids 59-71 sphingosine kinase 1 Homo sapiens 0-20 33771708-8 2021 Since sphingosine kinase 1 was overexpressed in keratinocytes incubated with the LTP-ligand complex, this enzyme might be responsible for the phosphorylation of the phytosphingosine fraction of the CPT-PHS ligand. phytosphingosine 165-181 sphingosine kinase 1 Homo sapiens 6-26 33817572-4 2021 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 0-20 33817572-4 2021 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 22-27 33817572-4 2021 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 0-20 33817572-4 2021 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 22-27 33817572-4 2021 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 102-105 sphingosine kinase 1 Homo sapiens 0-20 33817572-4 2021 Sphingosine kinase 1 (SphK1) catalyzes the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 102-105 sphingosine kinase 1 Homo sapiens 22-27 20598402-0 2010 Synthesis and bioactivity of sphingosine kinase inhibitors and their novel aspirinyl conjugated analogs. aspirinyl 75-84 sphingosine kinase 1 Homo sapiens 29-47 15923363-1 2005 Sphingosine kinase 1 (SK1) is a key enzyme critical to the sphingolipid metabolic pathway responsible for catalyzing the formation of the bioactive lipid sphingosine-1-phosphate. Sphingolipids 59-71 sphingosine kinase 1 Homo sapiens 22-25 15923363-1 2005 Sphingosine kinase 1 (SK1) is a key enzyme critical to the sphingolipid metabolic pathway responsible for catalyzing the formation of the bioactive lipid sphingosine-1-phosphate. sphingosine 1-phosphate 154-177 sphingosine kinase 1 Homo sapiens 0-20 15923363-1 2005 Sphingosine kinase 1 (SK1) is a key enzyme critical to the sphingolipid metabolic pathway responsible for catalyzing the formation of the bioactive lipid sphingosine-1-phosphate. sphingosine 1-phosphate 154-177 sphingosine kinase 1 Homo sapiens 22-25 15923363-2 2005 SK1-mediated production of sphingosine-1-phosphate has been shown to stimulate such biological processes as cell growth, differentiation, migration, angiogenesis, and inhibition of apoptosis. sphingosine 1-phosphate 27-50 sphingosine kinase 1 Homo sapiens 0-3 34549307-0 2021 SPHK1 contributes to cisplatin resistance in bladder cancer cells via the NONO/STAT3 axis. Cisplatin 21-30 sphingosine kinase 1 Homo sapiens 0-5 34838542-2 2022 We previously demonstrated that serine and glycine (SG) deprivation causes loss of sphingosine kinase 1 (SK1) in cancer cells, thereby increasing levels of its lipid substrate, sphingosine (Sph), which mediates several adaptive biological responses. Serine 32-38 sphingosine kinase 1 Homo sapiens 83-103 34838542-2 2022 We previously demonstrated that serine and glycine (SG) deprivation causes loss of sphingosine kinase 1 (SK1) in cancer cells, thereby increasing levels of its lipid substrate, sphingosine (Sph), which mediates several adaptive biological responses. Serine 32-38 sphingosine kinase 1 Homo sapiens 105-108 34838542-2 2022 We previously demonstrated that serine and glycine (SG) deprivation causes loss of sphingosine kinase 1 (SK1) in cancer cells, thereby increasing levels of its lipid substrate, sphingosine (Sph), which mediates several adaptive biological responses. Glycine 43-50 sphingosine kinase 1 Homo sapiens 83-103 34838542-2 2022 We previously demonstrated that serine and glycine (SG) deprivation causes loss of sphingosine kinase 1 (SK1) in cancer cells, thereby increasing levels of its lipid substrate, sphingosine (Sph), which mediates several adaptive biological responses. Glycine 43-50 sphingosine kinase 1 Homo sapiens 105-108 34838542-2 2022 We previously demonstrated that serine and glycine (SG) deprivation causes loss of sphingosine kinase 1 (SK1) in cancer cells, thereby increasing levels of its lipid substrate, sphingosine (Sph), which mediates several adaptive biological responses. Sphingosine 177-188 sphingosine kinase 1 Homo sapiens 83-103 34838542-2 2022 We previously demonstrated that serine and glycine (SG) deprivation causes loss of sphingosine kinase 1 (SK1) in cancer cells, thereby increasing levels of its lipid substrate, sphingosine (Sph), which mediates several adaptive biological responses. Sphingosine 177-188 sphingosine kinase 1 Homo sapiens 105-108 34838542-2 2022 We previously demonstrated that serine and glycine (SG) deprivation causes loss of sphingosine kinase 1 (SK1) in cancer cells, thereby increasing levels of its lipid substrate, sphingosine (Sph), which mediates several adaptive biological responses. Sphingosine 190-193 sphingosine kinase 1 Homo sapiens 83-103 34838542-2 2022 We previously demonstrated that serine and glycine (SG) deprivation causes loss of sphingosine kinase 1 (SK1) in cancer cells, thereby increasing levels of its lipid substrate, sphingosine (Sph), which mediates several adaptive biological responses. Sphingosine 190-193 sphingosine kinase 1 Homo sapiens 105-108 34838542-4 2022 Here, we identify 1-deoxysphinganine (dSA), a non-canonical sphingoid base generated in the absence of serine from the alternative condensation of alanine and palmitoyl CoA by serine palmitoyl transferase (SPT), as a proximal mediator of SG deprivation in SK1 loss and Sph level elevation in SG deprivation in cancer cells. spisulosine 18-36 sphingosine kinase 1 Homo sapiens 256-259 34838542-4 2022 Here, we identify 1-deoxysphinganine (dSA), a non-canonical sphingoid base generated in the absence of serine from the alternative condensation of alanine and palmitoyl CoA by serine palmitoyl transferase (SPT), as a proximal mediator of SG deprivation in SK1 loss and Sph level elevation in SG deprivation in cancer cells. spisulosine 38-41 sphingosine kinase 1 Homo sapiens 256-259 34838542-5 2022 SG starvation markedly increased dSA levels in vitro and in vivo, and in turn induced SK1 degradation through a SPT-dependent mechanism, resulting in an increase in SPH levels. spisulosine 33-36 sphingosine kinase 1 Homo sapiens 86-89 34865521-3 2022 We report that sphingosine-1-phosphate synthesis and expression is abundant in healthy trophoblast, whereas in pregnancies complicated by preeclampsia the placentae are associated with reduced sphingosine-1-phosphate and lower SPHK1 (sphingosine kinase 1) expression and activity. sphingosine 1-phosphate 15-38 sphingosine kinase 1 Homo sapiens 227-232 34865521-3 2022 We report that sphingosine-1-phosphate synthesis and expression is abundant in healthy trophoblast, whereas in pregnancies complicated by preeclampsia the placentae are associated with reduced sphingosine-1-phosphate and lower SPHK1 (sphingosine kinase 1) expression and activity. sphingosine 1-phosphate 15-38 sphingosine kinase 1 Homo sapiens 234-254 34549307-2 2021 Sphingosine kinase 1 (SPHK1) is a lipid kinase that phosphorylates sphingosine to generate S1P. Sphingosine 67-78 sphingosine kinase 1 Homo sapiens 0-20 34549307-2 2021 Sphingosine kinase 1 (SPHK1) is a lipid kinase that phosphorylates sphingosine to generate S1P. Sphingosine 67-78 sphingosine kinase 1 Homo sapiens 22-27 34549307-4 2021 However, whether SPHK1 is involved in the development of cisplatin resistance remains to be elucidated. Cisplatin 57-66 sphingosine kinase 1 Homo sapiens 17-22 34549307-8 2021 The results revealed that SPHK1 was positively correlated with cisplatin resistance in bladder cancer cells, exhibiting an antiapoptotic effect that was reflected by the downregulation of apoptosis-related proteins (Bax and cleaved caspase-3) and the upregulation of an antiapoptotic protein (Bcl-2) in SPHK1-overexpression cell lines. Cisplatin 63-72 sphingosine kinase 1 Homo sapiens 26-31 34549307-8 2021 The results revealed that SPHK1 was positively correlated with cisplatin resistance in bladder cancer cells, exhibiting an antiapoptotic effect that was reflected by the downregulation of apoptosis-related proteins (Bax and cleaved caspase-3) and the upregulation of an antiapoptotic protein (Bcl-2) in SPHK1-overexpression cell lines. Cisplatin 63-72 sphingosine kinase 1 Homo sapiens 303-308 34549307-9 2021 Suppression of SPHK1 by small interfering RNA or FTY-720 significantly reversed the antiapoptotic effect. Fingolimod Hydrochloride 49-56 sphingosine kinase 1 Homo sapiens 15-20 34549307-10 2021 A potential mechanism underlying SPHK1-induced cisplatin resistance and apoptosis inhibition may be activation of STAT3 via binding non-POU domain containing octamer binding. Cisplatin 47-56 sphingosine kinase 1 Homo sapiens 33-38 34549307-11 2021 In conclusion, the present study suggested that SPHK1 displayed significant antiapoptotic effects in cisplatin-based treatment, thus may serve as a potential novel therapeutic target for the treatment for bladder cancer. Cisplatin 101-110 sphingosine kinase 1 Homo sapiens 48-53 34737701-1 2021 Sphingosine kinase 1(SphK1) a key enzyme that catalyzes the conversion of sphingosine (Sph) to sphingosine 1-phosphate (S1P), so as to maintain the dynamic balance of sphingolipid-rheostat in cells and participate in cell growth and death, proliferation and migration, vasoconstriction and remodeling, inflammation and metabolism. Sphingosine 74-85 sphingosine kinase 1 Homo sapiens 0-20 34737701-1 2021 Sphingosine kinase 1(SphK1) a key enzyme that catalyzes the conversion of sphingosine (Sph) to sphingosine 1-phosphate (S1P), so as to maintain the dynamic balance of sphingolipid-rheostat in cells and participate in cell growth and death, proliferation and migration, vasoconstriction and remodeling, inflammation and metabolism. Sphingosine 74-85 sphingosine kinase 1 Homo sapiens 21-26 34737701-1 2021 Sphingosine kinase 1(SphK1) a key enzyme that catalyzes the conversion of sphingosine (Sph) to sphingosine 1-phosphate (S1P), so as to maintain the dynamic balance of sphingolipid-rheostat in cells and participate in cell growth and death, proliferation and migration, vasoconstriction and remodeling, inflammation and metabolism. Sphingosine 87-90 sphingosine kinase 1 Homo sapiens 0-20 34737701-1 2021 Sphingosine kinase 1(SphK1) a key enzyme that catalyzes the conversion of sphingosine (Sph) to sphingosine 1-phosphate (S1P), so as to maintain the dynamic balance of sphingolipid-rheostat in cells and participate in cell growth and death, proliferation and migration, vasoconstriction and remodeling, inflammation and metabolism. Sphingosine 87-90 sphingosine kinase 1 Homo sapiens 21-26 34737701-1 2021 Sphingosine kinase 1(SphK1) a key enzyme that catalyzes the conversion of sphingosine (Sph) to sphingosine 1-phosphate (S1P), so as to maintain the dynamic balance of sphingolipid-rheostat in cells and participate in cell growth and death, proliferation and migration, vasoconstriction and remodeling, inflammation and metabolism. sphingosine 1-phosphate 95-118 sphingosine kinase 1 Homo sapiens 0-20 34737701-1 2021 Sphingosine kinase 1(SphK1) a key enzyme that catalyzes the conversion of sphingosine (Sph) to sphingosine 1-phosphate (S1P), so as to maintain the dynamic balance of sphingolipid-rheostat in cells and participate in cell growth and death, proliferation and migration, vasoconstriction and remodeling, inflammation and metabolism. sphingosine 1-phosphate 95-118 sphingosine kinase 1 Homo sapiens 21-26 34737701-1 2021 Sphingosine kinase 1(SphK1) a key enzyme that catalyzes the conversion of sphingosine (Sph) to sphingosine 1-phosphate (S1P), so as to maintain the dynamic balance of sphingolipid-rheostat in cells and participate in cell growth and death, proliferation and migration, vasoconstriction and remodeling, inflammation and metabolism. sphingosine 1-phosphate 120-123 sphingosine kinase 1 Homo sapiens 0-20 34737701-1 2021 Sphingosine kinase 1(SphK1) a key enzyme that catalyzes the conversion of sphingosine (Sph) to sphingosine 1-phosphate (S1P), so as to maintain the dynamic balance of sphingolipid-rheostat in cells and participate in cell growth and death, proliferation and migration, vasoconstriction and remodeling, inflammation and metabolism. sphingosine 1-phosphate 120-123 sphingosine kinase 1 Homo sapiens 21-26 34418572-2 2021 Inhibition of sphingosine kinase isoform 1 (SK1) within this pathway, leads to a buildup of sphingosine and ceramide, two molecules directly linked to cell apoptosis, while decreasing the intracellular concentration of sphingosine-1-phosphate (S1P), a molecule linked to cellular proliferation. Sphingosine 92-103 sphingosine kinase 1 Homo sapiens 14-42 34639107-5 2021 The obtained data revealed significantly increased expression levels of activated sphingosine kinases (SphK1 and SphK2) in resistant cells concomitant with increased abundance of sphingosine-1-phosphate (S1P) and its precursor sphingosine, which was accompanied by increased expression levels of the enzymes regulating the ceramide salvage pathway, namely ceramide synthases 2 and 6 and acid ceramidase, especially after the exposure to vemurafenib. Vemurafenib 437-448 sphingosine kinase 1 Homo sapiens 103-108 34656931-0 2021 Overcoming enzalutamide resistance in metastatic prostate cancer by targeting sphingosine kinase. enzalutamide 11-23 sphingosine kinase 1 Homo sapiens 78-96 34656931-10 2021 SPHK inhibitors enhanced enzalutamide efficacy in PC cell lines. enzalutamide 25-37 sphingosine kinase 1 Homo sapiens 0-4 34418572-2 2021 Inhibition of sphingosine kinase isoform 1 (SK1) within this pathway, leads to a buildup of sphingosine and ceramide, two molecules directly linked to cell apoptosis, while decreasing the intracellular concentration of sphingosine-1-phosphate (S1P), a molecule linked to cellular proliferation. Sphingosine 92-103 sphingosine kinase 1 Homo sapiens 44-47 34418572-2 2021 Inhibition of sphingosine kinase isoform 1 (SK1) within this pathway, leads to a buildup of sphingosine and ceramide, two molecules directly linked to cell apoptosis, while decreasing the intracellular concentration of sphingosine-1-phosphate (S1P), a molecule linked to cellular proliferation. Ceramides 108-116 sphingosine kinase 1 Homo sapiens 14-42 34656931-11 2021 Transcriptomic and lipidomic analyses indicated that enzalutamide combined with SPHK inhibition enhanced PC cell death by SREBP-induced lipotoxicity. srebp 122-127 sphingosine kinase 1 Homo sapiens 80-84 34418572-2 2021 Inhibition of sphingosine kinase isoform 1 (SK1) within this pathway, leads to a buildup of sphingosine and ceramide, two molecules directly linked to cell apoptosis, while decreasing the intracellular concentration of sphingosine-1-phosphate (S1P), a molecule linked to cellular proliferation. Ceramides 108-116 sphingosine kinase 1 Homo sapiens 44-47 34656931-12 2021 INTERPRETATION: Ceramide-S1P signalling promotes ARSI resistance, which can be reversed with SPHK inhibitors. Ceramides 16-24 sphingosine kinase 1 Homo sapiens 93-97 34418572-2 2021 Inhibition of sphingosine kinase isoform 1 (SK1) within this pathway, leads to a buildup of sphingosine and ceramide, two molecules directly linked to cell apoptosis, while decreasing the intracellular concentration of sphingosine-1-phosphate (S1P), a molecule linked to cellular proliferation. sphingosine 1-phosphate 219-242 sphingosine kinase 1 Homo sapiens 14-42 34418572-2 2021 Inhibition of sphingosine kinase isoform 1 (SK1) within this pathway, leads to a buildup of sphingosine and ceramide, two molecules directly linked to cell apoptosis, while decreasing the intracellular concentration of sphingosine-1-phosphate (S1P), a molecule linked to cellular proliferation. sphingosine 1-phosphate 219-242 sphingosine kinase 1 Homo sapiens 44-47 34418572-2 2021 Inhibition of sphingosine kinase isoform 1 (SK1) within this pathway, leads to a buildup of sphingosine and ceramide, two molecules directly linked to cell apoptosis, while decreasing the intracellular concentration of sphingosine-1-phosphate (S1P), a molecule linked to cellular proliferation. sphingosine 1-phosphate 244-247 sphingosine kinase 1 Homo sapiens 14-42 34418572-2 2021 Inhibition of sphingosine kinase isoform 1 (SK1) within this pathway, leads to a buildup of sphingosine and ceramide, two molecules directly linked to cell apoptosis, while decreasing the intracellular concentration of sphingosine-1-phosphate (S1P), a molecule linked to cellular proliferation. sphingosine 1-phosphate 244-247 sphingosine kinase 1 Homo sapiens 44-47 34418572-4 2021 A set of compounds designed to assess the impact of synthetic modifications to the hydroxynaphthalene ring region of the template inhibitor with SK1 to obtain a compound with increased efficacy in vivo. Naphthols 83-101 sphingosine kinase 1 Homo sapiens 145-148 34530846-1 2021 BACKGROUND: Sphingosine Kinase (SphK) that catalyzes sphingosine (Sph) to sphingosine 1-phosphate (S1P), plays a key role in both sphingolipid metabolism and cellular signaling. Sphingosine 53-64 sphingosine kinase 1 Homo sapiens 12-30 34659894-0 2021 Sphingosine 1-phosphate (S1P) produced by sphingosine kinase 1 (SphK1) and exported via ABCC1 is related to hepatocellular carcinoma (HCC) progression. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 42-62 34659894-0 2021 Sphingosine 1-phosphate (S1P) produced by sphingosine kinase 1 (SphK1) and exported via ABCC1 is related to hepatocellular carcinoma (HCC) progression. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 64-69 34659894-1 2021 Sphingosine-1-Phosphate (S1P) is produced by Sphingosine Kinase 1 (SphK1) in the cell and is transported out of the cells by ABCC1 transporter. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 45-65 34659894-1 2021 Sphingosine-1-Phosphate (S1P) is produced by Sphingosine Kinase 1 (SphK1) in the cell and is transported out of the cells by ABCC1 transporter. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 67-72 34659894-1 2021 Sphingosine-1-Phosphate (S1P) is produced by Sphingosine Kinase 1 (SphK1) in the cell and is transported out of the cells by ABCC1 transporter. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 45-65 34659894-1 2021 Sphingosine-1-Phosphate (S1P) is produced by Sphingosine Kinase 1 (SphK1) in the cell and is transported out of the cells by ABCC1 transporter. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 67-72 34656931-12 2021 INTERPRETATION: Ceramide-S1P signalling promotes ARSI resistance, which can be reversed with SPHK inhibitors. arsi 49-53 sphingosine kinase 1 Homo sapiens 93-97 34530846-1 2021 BACKGROUND: Sphingosine Kinase (SphK) that catalyzes sphingosine (Sph) to sphingosine 1-phosphate (S1P), plays a key role in both sphingolipid metabolism and cellular signaling. Sphingosine 53-64 sphingosine kinase 1 Homo sapiens 32-36 34530846-1 2021 BACKGROUND: Sphingosine Kinase (SphK) that catalyzes sphingosine (Sph) to sphingosine 1-phosphate (S1P), plays a key role in both sphingolipid metabolism and cellular signaling. Sphingosine 66-69 sphingosine kinase 1 Homo sapiens 12-30 34530846-1 2021 BACKGROUND: Sphingosine Kinase (SphK) that catalyzes sphingosine (Sph) to sphingosine 1-phosphate (S1P), plays a key role in both sphingolipid metabolism and cellular signaling. Sphingosine 66-69 sphingosine kinase 1 Homo sapiens 32-36 34530846-1 2021 BACKGROUND: Sphingosine Kinase (SphK) that catalyzes sphingosine (Sph) to sphingosine 1-phosphate (S1P), plays a key role in both sphingolipid metabolism and cellular signaling. sphingosine 1-phosphate 74-97 sphingosine kinase 1 Homo sapiens 12-30 34530846-1 2021 BACKGROUND: Sphingosine Kinase (SphK) that catalyzes sphingosine (Sph) to sphingosine 1-phosphate (S1P), plays a key role in both sphingolipid metabolism and cellular signaling. sphingosine 1-phosphate 74-97 sphingosine kinase 1 Homo sapiens 32-36 34530846-1 2021 BACKGROUND: Sphingosine Kinase (SphK) that catalyzes sphingosine (Sph) to sphingosine 1-phosphate (S1P), plays a key role in both sphingolipid metabolism and cellular signaling. sphingosine 1-phosphate 99-102 sphingosine kinase 1 Homo sapiens 12-30 34530846-1 2021 BACKGROUND: Sphingosine Kinase (SphK) that catalyzes sphingosine (Sph) to sphingosine 1-phosphate (S1P), plays a key role in both sphingolipid metabolism and cellular signaling. sphingosine 1-phosphate 99-102 sphingosine kinase 1 Homo sapiens 32-36 34530846-4 2021 METHODS: Levels of SphK-related sphingolipid metabolites, including Sph, S1P, dihydrosphingosine (dhSph) and dihydro-S1P (dhS1P) in serum were measured by targeted-lipidomic analyses. Sphingolipids 32-44 sphingosine kinase 1 Homo sapiens 19-23 34530846-4 2021 METHODS: Levels of SphK-related sphingolipid metabolites, including Sph, S1P, dihydrosphingosine (dhSph) and dihydro-S1P (dhS1P) in serum were measured by targeted-lipidomic analyses. Sphingosine 68-71 sphingosine kinase 1 Homo sapiens 19-23 34530846-4 2021 METHODS: Levels of SphK-related sphingolipid metabolites, including Sph, S1P, dihydrosphingosine (dhSph) and dihydro-S1P (dhS1P) in serum were measured by targeted-lipidomic analyses. safingol 78-96 sphingosine kinase 1 Homo sapiens 19-23 34530846-4 2021 METHODS: Levels of SphK-related sphingolipid metabolites, including Sph, S1P, dihydrosphingosine (dhSph) and dihydro-S1P (dhS1P) in serum were measured by targeted-lipidomic analyses. safingol 98-103 sphingosine kinase 1 Homo sapiens 19-23 34530846-4 2021 METHODS: Levels of SphK-related sphingolipid metabolites, including Sph, S1P, dihydrosphingosine (dhSph) and dihydro-S1P (dhS1P) in serum were measured by targeted-lipidomic analyses. dihydrosphingosine 1-phosphate 109-120 sphingosine kinase 1 Homo sapiens 19-23 34530846-4 2021 METHODS: Levels of SphK-related sphingolipid metabolites, including Sph, S1P, dihydrosphingosine (dhSph) and dihydro-S1P (dhS1P) in serum were measured by targeted-lipidomic analyses. dihydrosphingosine 1-phosphate 122-127 sphingosine kinase 1 Homo sapiens 19-23 34415509-6 2021 Blocking phospholipase D activity attenuated the LPA-induced activation of SphK1 and the synthesis of ABCC1 and ABCG2 transporters that secrete S1P from cells. lpa 49-52 sphingosine kinase 1 Homo sapiens 75-80 34128259-0 2021 Design, synthesis, and biological evaluation of novel benzimidazole derivatives as sphingosine kinase 1 inhibitor. benzimidazole 54-67 sphingosine kinase 1 Homo sapiens 83-103 34128259-3 2021 In this study, a novel series of benzimidazoles was synthesized and evaluated as SphK1 inhibitors. Benzimidazoles 33-47 sphingosine kinase 1 Homo sapiens 81-86 34128259-4 2021 Our design strategy is twofold: It aimed first to study the effect of replacing the 5-position of the benzimidazole ring with a polar carboxylic acid group on the SphK1-inhibitory activity and cytotoxicity. benzimidazole 102-115 sphingosine kinase 1 Homo sapiens 163-168 34128259-7 2021 The binding affinity of the benzimidazole derivatives toward SphK1 was measured by fluorescence binding and molecular docking. benzimidazole 28-41 sphingosine kinase 1 Homo sapiens 61-66 34128259-12 2021 Molecular docking revealed that most of these compounds fit well into the ATP-binding site of SphK1 and form hydrogen bond interactions with catalytically important residues. Adenosine Triphosphate 74-77 sphingosine kinase 1 Homo sapiens 94-99 34128259-12 2021 Molecular docking revealed that most of these compounds fit well into the ATP-binding site of SphK1 and form hydrogen bond interactions with catalytically important residues. Hydrogen 109-117 sphingosine kinase 1 Homo sapiens 94-99 34128259-13 2021 Overall, the findings suggest the therapeutic potential of benzimidazoles in the clinical management of SphK1-associated diseases. Benzimidazoles 59-73 sphingosine kinase 1 Homo sapiens 104-109 34085165-1 2021 Sphingosine-1-phosphate (S1P), a bioactive lipid mediator, is generated from sphingosine by sphingosine kinases (SPHKs) 1 and 2 and is metabolized to 2-hexadecenal ( 2-HDE) and ethanolamine phosphate by S1P lyase (S1PL) in mammalian cells. Sphingosine 77-88 sphingosine kinase 1 Homo sapiens 92-127 34133010-2 2021 The sphingosine kinase 1 (SPHK1)/sphingosine-1 phosphate/sphingosine-1 phosphate receptor 2 pathway promotes vascular remodeling and induces PAH. sphingosine 1-phosphate 33-56 sphingosine kinase 1 Homo sapiens 4-24 34133010-2 2021 The sphingosine kinase 1 (SPHK1)/sphingosine-1 phosphate/sphingosine-1 phosphate receptor 2 pathway promotes vascular remodeling and induces PAH. sphingosine 1-phosphate 33-56 sphingosine kinase 1 Homo sapiens 26-31 34415509-9 2021 Doxorubicin-resistant MCF-7 cells had increased S1P2 and S1P3 receptor expression and show increased LPA-induced SphK1 activation, increased expression of ABCC1, ABCG2 and greater S1P secretion. Doxorubicin 0-11 sphingosine kinase 1 Homo sapiens 113-118 34085165-1 2021 Sphingosine-1-phosphate (S1P), a bioactive lipid mediator, is generated from sphingosine by sphingosine kinases (SPHKs) 1 and 2 and is metabolized to 2-hexadecenal ( 2-HDE) and ethanolamine phosphate by S1P lyase (S1PL) in mammalian cells. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 92-127 34415509-9 2021 Doxorubicin-resistant MCF-7 cells had increased S1P2 and S1P3 receptor expression and show increased LPA-induced SphK1 activation, increased expression of ABCC1, ABCG2 and greater S1P secretion. lpa 101-104 sphingosine kinase 1 Homo sapiens 113-118 34153838-5 2021 Among the three strained alkene amino acids examined, both SphK and 2"-aTCOK offered successful fluorescent labeling of GCGR ICL3 with the appropriate tetrazine probes. 1,2,3,4-tetrazine 151-160 sphingosine kinase 1 Homo sapiens 59-63 34502095-0 2021 A Dansyl-Modified Sphingosine Kinase Inhibitor DPF-543 Enhanced De Novo Ceramide Generation. dpf-543 47-54 sphingosine kinase 1 Homo sapiens 18-36 34502095-0 2021 A Dansyl-Modified Sphingosine Kinase Inhibitor DPF-543 Enhanced De Novo Ceramide Generation. Ceramides 72-80 sphingosine kinase 1 Homo sapiens 18-36 34502095-1 2021 Sphingosine-1-phosphate (S1P) synthesized by sphingosine kinase (SPHK) is a signaling molecule, involved in cell proliferation, growth, differentiation, and survival. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 45-63 34502095-1 2021 Sphingosine-1-phosphate (S1P) synthesized by sphingosine kinase (SPHK) is a signaling molecule, involved in cell proliferation, growth, differentiation, and survival. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 65-69 34502095-1 2021 Sphingosine-1-phosphate (S1P) synthesized by sphingosine kinase (SPHK) is a signaling molecule, involved in cell proliferation, growth, differentiation, and survival. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 45-63 34502095-1 2021 Sphingosine-1-phosphate (S1P) synthesized by sphingosine kinase (SPHK) is a signaling molecule, involved in cell proliferation, growth, differentiation, and survival. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 65-69 34502095-3 2021 In this regard, SPHK/S1P axis regulation has been a specific issue in the anticancer strategy to turn accumulated sphingosine (SPN) into cytotoxic ceramides (Cers). Sphingosine 114-125 sphingosine kinase 1 Homo sapiens 16-20 34502095-3 2021 In this regard, SPHK/S1P axis regulation has been a specific issue in the anticancer strategy to turn accumulated sphingosine (SPN) into cytotoxic ceramides (Cers). Sphingosine 127-130 sphingosine kinase 1 Homo sapiens 16-20 34502095-3 2021 In this regard, SPHK/S1P axis regulation has been a specific issue in the anticancer strategy to turn accumulated sphingosine (SPN) into cytotoxic ceramides (Cers). Ceramides 147-156 sphingosine kinase 1 Homo sapiens 16-20 34502095-3 2021 In this regard, SPHK/S1P axis regulation has been a specific issue in the anticancer strategy to turn accumulated sphingosine (SPN) into cytotoxic ceramides (Cers). Ceramides 158-162 sphingosine kinase 1 Homo sapiens 16-20 34502095-12 2021 Our results demonstrated that the structural modification on SPHK inhibitors is still an attractive anticancer strategy by regulating sphingolipid metabolism. Sphingolipids 134-146 sphingosine kinase 1 Homo sapiens 61-65 34440937-3 2021 Phosphorylation of sphingosine kinase 1 (SphK1) produces sphingosine-1-phosphate (S1P), which increases inflammatory cytokine production, although the role of S1P in RA angiogenesis is unclear. sphingosine 1-phosphate 57-80 sphingosine kinase 1 Homo sapiens 19-39 34440937-3 2021 Phosphorylation of sphingosine kinase 1 (SphK1) produces sphingosine-1-phosphate (S1P), which increases inflammatory cytokine production, although the role of S1P in RA angiogenesis is unclear. sphingosine 1-phosphate 57-80 sphingosine kinase 1 Homo sapiens 41-46 34419228-7 2021 Metabolomics analysis demonstrated that sphingosine-1-phosphate, which is regulated by sphingosine kinase 1 and sphingosine kinase 2, is a differential metabolite in plasma between the WJD-treated group and the control group, participating in the sphingolipid signaling. sphingosine 1-phosphate 40-63 sphingosine kinase 1 Homo sapiens 87-107 34419228-7 2021 Metabolomics analysis demonstrated that sphingosine-1-phosphate, which is regulated by sphingosine kinase 1 and sphingosine kinase 2, is a differential metabolite in plasma between the WJD-treated group and the control group, participating in the sphingolipid signaling. Sphingolipids 247-259 sphingosine kinase 1 Homo sapiens 87-107 34419228-9 2021 Through a series of validation assays, Tricin inhibited the tumor growth mainly by suppressing PRKCA/SPHK/S1P signaling and antiapoptotic signaling. tricin 39-45 sphingosine kinase 1 Homo sapiens 101-105 34382511-0 2022 SK1 inhibitor RB005 Induces Apoptosis in Colorectal Cancer Cells through SK1 Inhibition Dependent and Independent Pathway. 1-(4-octylphenethyl)piperidin-4-ol 14-19 sphingosine kinase 1 Homo sapiens 0-3 34382511-0 2022 SK1 inhibitor RB005 Induces Apoptosis in Colorectal Cancer Cells through SK1 Inhibition Dependent and Independent Pathway. 1-(4-octylphenethyl)piperidin-4-ol 14-19 sphingosine kinase 1 Homo sapiens 73-76 34382511-2 2022 Sphingosine-1-phosphate (S1P) is a bioactive lipid mediator that plays critical roles in cancer cell proliferation, migration, and angiogenesis converted by the isoforms of sphingosine kinase (SK1 and SK2). sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 193-196 34382511-2 2022 Sphingosine-1-phosphate (S1P) is a bioactive lipid mediator that plays critical roles in cancer cell proliferation, migration, and angiogenesis converted by the isoforms of sphingosine kinase (SK1 and SK2). sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 193-196 34382511-3 2022 SK1 is highly expressed in colorectal cancer, its inhibitors suppress the formation of S1P and increase ceramide levels having a pro-apoptotic function. Ceramides 104-112 sphingosine kinase 1 Homo sapiens 0-3 34382511-4 2022 RB005 is a selective SK1 inhibitor and a structural analog of PP2A activator FTY720. 1-(4-octylphenethyl)piperidin-4-ol 0-5 sphingosine kinase 1 Homo sapiens 21-24 34122632-4 2021 The results of cell proliferation assay and reverse transcription-PCR demonstrated that ATRA may exert synergistic effects with the SphK1 inhibitor SKI 5C or the pan-SphK inhibitor SKI II to inhibit the proliferation of K562 cells and upregulate the expression levels of the ATRA-inducible enzyme cytochrome P450 26A1 (CYP26A1). Tretinoin 88-92 sphingosine kinase 1 Homo sapiens 132-137 34122632-4 2021 The results of cell proliferation assay and reverse transcription-PCR demonstrated that ATRA may exert synergistic effects with the SphK1 inhibitor SKI 5C or the pan-SphK inhibitor SKI II to inhibit the proliferation of K562 cells and upregulate the expression levels of the ATRA-inducible enzyme cytochrome P450 26A1 (CYP26A1). Tretinoin 88-92 sphingosine kinase 1 Homo sapiens 166-170 34122632-4 2021 The results of cell proliferation assay and reverse transcription-PCR demonstrated that ATRA may exert synergistic effects with the SphK1 inhibitor SKI 5C or the pan-SphK inhibitor SKI II to inhibit the proliferation of K562 cells and upregulate the expression levels of the ATRA-inducible enzyme cytochrome P450 26A1 (CYP26A1). Tretinoin 275-279 sphingosine kinase 1 Homo sapiens 132-137 34122632-4 2021 The results of cell proliferation assay and reverse transcription-PCR demonstrated that ATRA may exert synergistic effects with the SphK1 inhibitor SKI 5C or the pan-SphK inhibitor SKI II to inhibit the proliferation of K562 cells and upregulate the expression levels of the ATRA-inducible enzyme cytochrome P450 26A1 (CYP26A1). Tretinoin 275-279 sphingosine kinase 1 Homo sapiens 166-170 34122632-5 2021 Knocking down the expression of SphK1 or SphK2 in K562 cells by small interfering RNA enhanced the inhibitory effects of ATRA and induced the expression of CYP26A1. Tretinoin 121-125 sphingosine kinase 1 Homo sapiens 32-37 34152633-0 2021 Geniposide downregulates the VEGF/SphK1/S1P pathway and alleviates angiogenesis in rheumatoid arthritis in vivo and in vitro. geniposide 0-10 sphingosine kinase 1 Homo sapiens 34-39 34262394-2 2021 Sphingosine kinases (SPHK1 and SPHK2) synthesize sphingosine-1-phosphate (S1P), which works either intracellularly or extracellularly via its receptors S1PR1-5 in an autocrine or paracrine manner. sphingosine 1-phosphate 49-72 sphingosine kinase 1 Homo sapiens 21-26 34201962-4 2021 Notably, we demonstrated that EGFR+ cells are resistant to temozolomide (TMZ), the standard chemotherapeutic drug in GBM treatment, and the inhibition of SK1 or S1P receptors made EGFR+ cells sensitive to TMZ; moreover, exogenous S1P reverted this effect, thus involving extracellular S1P as a survival signal in TMZ resistance in GBM cells. Temozolomide 205-208 sphingosine kinase 1 Homo sapiens 154-157 34201962-4 2021 Notably, we demonstrated that EGFR+ cells are resistant to temozolomide (TMZ), the standard chemotherapeutic drug in GBM treatment, and the inhibition of SK1 or S1P receptors made EGFR+ cells sensitive to TMZ; moreover, exogenous S1P reverted this effect, thus involving extracellular S1P as a survival signal in TMZ resistance in GBM cells. Temozolomide 313-316 sphingosine kinase 1 Homo sapiens 154-157 34201962-5 2021 In addition, both PI3K/AKT and MAPK inhibitors markedly reduced cell survival, suggesting that the enhanced resistance to TMZ of EGFR+ cells is dependent on the increased S1P secretion, downstream of the EGFR-ERK-SK1-S1P pathway. Temozolomide 122-125 sphingosine kinase 1 Homo sapiens 213-216 34135730-5 2021 Adenosine and sphingosine kinase/sphingosine 1-phosphate signaling axes (SphK/S1P) play important roles in remyelination processes. sphingosine 1-phosphate 33-56 sphingosine kinase 1 Homo sapiens 73-77 34749611-0 2021 High-resolution MD simulation studies to get mechanistic insights into the urea-induced denaturation of human Sphingosine kinase 1. Urea 75-79 sphingosine kinase 1 Homo sapiens 110-130 34749611-3 2021 We found that SPhK1 showed a biphasic unfolding with an intermediate state (~ 4.0 M urea). Urea 84-88 sphingosine kinase 1 Homo sapiens 14-19 34749611-7 2021 RESULTS: The results suggested a constant form of the unfolding of SPhK1 upon extending the simulation time scale at different urea concentrations. Urea 127-131 sphingosine kinase 1 Homo sapiens 67-72 34749611-8 2021 Furthermore, we showed step by step unfolding and percentage of secondary structure contents in SPhK1 under the influence of urea at each concentration. Urea 125-129 sphingosine kinase 1 Homo sapiens 96-101 34749611-9 2021 CONCLUSION: The results from the current work revealed a uniform pattern of the SPhK1 unfolding at different urea concentrations. Urea 109-113 sphingosine kinase 1 Homo sapiens 80-85 34749611-10 2021 This study provides deeper mechanistic insights into the urea-induced denaturation of SPhK1. Urea 57-61 sphingosine kinase 1 Homo sapiens 86-91 35489137-0 2022 Stimulating the expression of sphingosine kinase 1 (SphK1) is beneficial to reduce acrylamide-induced nerve cell damage. Acrylamide 83-93 sphingosine kinase 1 Homo sapiens 30-50 35489137-0 2022 Stimulating the expression of sphingosine kinase 1 (SphK1) is beneficial to reduce acrylamide-induced nerve cell damage. Acrylamide 83-93 sphingosine kinase 1 Homo sapiens 52-57 35489137-3 2022 The purpose of this study was to investigate the role and potential mechanism of SphK1 in ACR-induced nerve injury. Acrylamide 90-93 sphingosine kinase 1 Homo sapiens 81-86 35489137-5 2022 For in vitro experiments, SphK1 in human SH-SY5Y neuroblastoma cells was activated using SphK1-specific activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 114-145 sphingosine kinase 1 Homo sapiens 26-31 35489137-5 2022 For in vitro experiments, SphK1 in human SH-SY5Y neuroblastoma cells was activated using SphK1-specific activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 114-145 sphingosine kinase 1 Homo sapiens 89-94 35489137-5 2022 For in vitro experiments, SphK1 in human SH-SY5Y neuroblastoma cells was activated using SphK1-specific activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 147-150 sphingosine kinase 1 Homo sapiens 26-31 35489137-5 2022 For in vitro experiments, SphK1 in human SH-SY5Y neuroblastoma cells was activated using SphK1-specific activator phorbol 12-myristate 13-acetate (PMA). Tetradecanoylphorbol Acetate 147-150 sphingosine kinase 1 Homo sapiens 89-94 35489137-7 2022 The results of the population study showed that the contents of SphK1 and S1P in the ACR-exposed occupational contact group were lower than in the non-exposed group. Acrylamide 85-88 sphingosine kinase 1 Homo sapiens 64-69 35489137-8 2022 The results of in vitro experiments showed that expression of SphK1 decreased with the increase in ACR concentration. Acrylamide 99-102 sphingosine kinase 1 Homo sapiens 62-67 35489137-11 2022 These results suggest that activating SphK1 can protect against nerve cell damage caused by ACR. Acrylamide 92-95 sphingosine kinase 1 Homo sapiens 38-43 35630821-3 2022 Although PF-543 strongly and selectively inhibits SK1, its anticancer effect is not high, and the underlying reason remains difficult to explain. PF-543 9-15 sphingosine kinase 1 Homo sapiens 50-53 35289120-1 2022 SPHK1 (sphingosine kinase-1) catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), is found to be highly expressed in solid tumors. Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 0-5 35289120-1 2022 SPHK1 (sphingosine kinase-1) catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), is found to be highly expressed in solid tumors. Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 7-27 35289120-1 2022 SPHK1 (sphingosine kinase-1) catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), is found to be highly expressed in solid tumors. sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 0-5 35289120-1 2022 SPHK1 (sphingosine kinase-1) catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), is found to be highly expressed in solid tumors. sphingosine 1-phosphate 77-100 sphingosine kinase 1 Homo sapiens 7-27 35289120-1 2022 SPHK1 (sphingosine kinase-1) catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), is found to be highly expressed in solid tumors. sphingosine 1-phosphate 102-105 sphingosine kinase 1 Homo sapiens 0-5 35289120-1 2022 SPHK1 (sphingosine kinase-1) catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), is found to be highly expressed in solid tumors. sphingosine 1-phosphate 102-105 sphingosine kinase 1 Homo sapiens 7-27 35129008-8 2022 Treatment of FL cells with LY294002, the inhibitor of Akt, could delete the MF-induced SK1 activation under the condition of calcium-free medium. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 27-35 sphingosine kinase 1 Homo sapiens 87-90 35418289-8 2022 Finally, docking studies on sphingosine kinase 1 (PDB ID: 3VZB) of sphingosine were carried out. Sphingosine 67-78 sphingosine kinase 1 Homo sapiens 28-48 35418289-11 2022 The UHPLC-MS/MS of ethanolic and the ethyl acetate extract showed that this extract has compound such as sphinganine C16, N,N-Dimethylphingosine compound, then could be possible that the effect observed be due to their metabolites which could be ligands for the sphingosine kinase 1 as was demonstrated by docking studies. safingol 105-116 sphingosine kinase 1 Homo sapiens 262-282 35562234-4 2022 RESULTS: In adenomyotic samples, the expression of sphingosine kinase 1 (SPHK1), the enzyme responsible for the synthesis of S1P, and of S1P phosphatase 2 (SGPP2), the enzyme responsible for the conversion of S1P back to sphingosine, was lower (P = 0.0006; P = 0.0015), whereas that of calcium and integrin-binding protein 1, responsible for membrane translocation of SPHK1, was higher (P = 0.0001) compared with healthy controls. Sphingosine 221-232 sphingosine kinase 1 Homo sapiens 51-71 35129008-6 2022 Moreover, MF-activated SK1 could be completely inhibited by BAPTA, and MF-induced cell proliferation was abolished by SKI II, the specific inhibitor of SK1. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 60-65 sphingosine kinase 1 Homo sapiens 23-26 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. Phosphates 59-68 sphingosine kinase 1 Homo sapiens 0-18 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. Phosphates 59-68 sphingosine kinase 1 Homo sapiens 20-24 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. Adenosine Triphosphate 74-77 sphingosine kinase 1 Homo sapiens 0-18 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. Adenosine Triphosphate 74-77 sphingosine kinase 1 Homo sapiens 20-24 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. Sphingosine 81-92 sphingosine kinase 1 Homo sapiens 0-18 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. Sphingosine 81-92 sphingosine kinase 1 Homo sapiens 20-24 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. Sphingosine 94-97 sphingosine kinase 1 Homo sapiens 0-18 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. Sphingosine 94-97 sphingosine kinase 1 Homo sapiens 20-24 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. sphingosine 1-phosphate 110-133 sphingosine kinase 1 Homo sapiens 0-18 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. sphingosine 1-phosphate 110-133 sphingosine kinase 1 Homo sapiens 20-24 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. sphingosine 1-phosphate 135-138 sphingosine kinase 1 Homo sapiens 0-18 35219046-1 2022 Sphingosine kinase (SphK), which catalyzes the transfer of phosphate from ATP to sphingosine (Sph) generating sphingosine-1-phosphate (S1P) has emerged as therapeutic target since the discovery of connections of S1P with cancer progress. sphingosine 1-phosphate 135-138 sphingosine kinase 1 Homo sapiens 20-24 35129008-8 2022 Treatment of FL cells with LY294002, the inhibitor of Akt, could delete the MF-induced SK1 activation under the condition of calcium-free medium. Calcium 125-132 sphingosine kinase 1 Homo sapiens 87-90 35094989-7 2022 In the presence of Sphingosine kinase (SphK1) inhibitor, adenosine mediated effects on ATP release were abolished. Adenosine 57-66 sphingosine kinase 1 Homo sapiens 39-44 34993846-3 2022 Sphingosine kinase 1 (Sphk1) is a key enzyme in the regulation of sphingosine metabolism involved in the ferroptosis pathway, but its role in ICH needs clarification. Sphingosine 66-77 sphingosine kinase 1 Homo sapiens 0-20 34993846-3 2022 Sphingosine kinase 1 (Sphk1) is a key enzyme in the regulation of sphingosine metabolism involved in the ferroptosis pathway, but its role in ICH needs clarification. Sphingosine 66-77 sphingosine kinase 1 Homo sapiens 22-27 34993846-6 2022 Mechanistically, this study unveiled that sphingosine kinase 1/sphingosine 1-phosphate/extracellular-regulated protein kinases/phosphorylated extracellular-regulated protein kinases (Sphk1/S1p/ERK/p-ERK) pathway is responsible for regulation of ferroptosis leading to secondary brain injury and cell death following ICH. sphingosine 1-phosphate 63-86 sphingosine kinase 1 Homo sapiens 183-188 35184376-9 2022 Furthermore, sphingosine 1-phosphate produced by SphK1 can modulate REST protein turnover via MAPK signaling pathway. sphingosine 1-phosphate 13-36 sphingosine kinase 1 Homo sapiens 49-54 35007762-7 2022 Additionally, we showed that PA interacted with and positively regulated sphingosine kinase 1 (SPHK1). Phosphatidic Acids 29-31 sphingosine kinase 1 Homo sapiens 73-93 35007762-7 2022 Additionally, we showed that PA interacted with and positively regulated sphingosine kinase 1 (SPHK1). Phosphatidic Acids 29-31 sphingosine kinase 1 Homo sapiens 95-100 35059910-2 2022 However, how resveratrol exerts its anti-leukemic actions by modulating anti-apoptotic ceramide catabolism enzymes, mainly sphingosine kinase (SK-1) and glucosylceramide synthase (GCS), in FLT3-ITD AML remains unclear. Resveratrol 13-24 sphingosine kinase 1 Homo sapiens 143-147 35059910-2 2022 However, how resveratrol exerts its anti-leukemic actions by modulating anti-apoptotic ceramide catabolism enzymes, mainly sphingosine kinase (SK-1) and glucosylceramide synthase (GCS), in FLT3-ITD AML remains unclear. Ceramides 87-95 sphingosine kinase 1 Homo sapiens 143-147 35059910-5 2022 Caspase-3 and PARP activation, changes in SK-1 and GCS levels by resveratrol alone or PARP cleavage in co-treatments were determined by western blot. Resveratrol 65-76 sphingosine kinase 1 Homo sapiens 42-46 35059910-7 2022 Resveratrol downregulated SK-1 and GCS expression in both cell lines. Resveratrol 0-11 sphingosine kinase 1 Homo sapiens 26-30 35158767-3 2022 FTY720 (Fingolimod), which targets SphK1 and S1P1, prevented HIF-1alpha accumulation, and also inhibited cell proliferation in both normoxia and hypoxia unlike conventional chemotherapy. Fingolimod Hydrochloride 8-18 sphingosine kinase 1 Homo sapiens 35-40 35203362-3 2022 S1P is a bioactive sphingolipid synthesized by the phosphorylation of sphingosine catalyzed by sphingosine kinase (SK) 1 and 2. Sphingolipids 19-31 sphingosine kinase 1 Homo sapiens 95-126 35203362-3 2022 S1P is a bioactive sphingolipid synthesized by the phosphorylation of sphingosine catalyzed by sphingosine kinase (SK) 1 and 2. Sphingosine 70-81 sphingosine kinase 1 Homo sapiens 95-126 35203362-7 2022 Notably, the involvement of S1P axis in Adn action was highlighted since, when SK1 and 2 were inhibited by PF543 and ABC294640 inhibitors, respectively, not only the electrophysiological changes but also the increase of oxygen consumption and of aminoacid levels induced by the hormone, were significantly inhibited. 3-(4-chlorophenyl)-adamantane-1-carboxylic acid (pyridin-4-ylmethyl)amide 117-126 sphingosine kinase 1 Homo sapiens 79-88 35203362-7 2022 Notably, the involvement of S1P axis in Adn action was highlighted since, when SK1 and 2 were inhibited by PF543 and ABC294640 inhibitors, respectively, not only the electrophysiological changes but also the increase of oxygen consumption and of aminoacid levels induced by the hormone, were significantly inhibited. Oxygen 220-226 sphingosine kinase 1 Homo sapiens 79-88 35237148-13 2021 Results: In this study, we found that SPHK inhibitors (ABC294640 and FTY720) combined with ZA decreased the degree of inflammatory osteolysis in vivo. 3-(4-chlorophenyl)-adamantane-1-carboxylic acid (pyridin-4-ylmethyl)amide 55-64 sphingosine kinase 1 Homo sapiens 38-42 35237148-13 2021 Results: In this study, we found that SPHK inhibitors (ABC294640 and FTY720) combined with ZA decreased the degree of inflammatory osteolysis in vivo. Fingolimod Hydrochloride 69-75 sphingosine kinase 1 Homo sapiens 38-42 35237148-16 2021 Conclusion: Our study revealed that wear particles could induce inflammatory osteolysis by upregulating SPHKs/S1P-TRAF2-BECN1 and SPHK inhibitors/ZA inhibit osteoclastogenesis in vitro and prevent inflammatory osteolysis in vivo, suggesting that SPHK inhibitors and ZA can be a new perspective and scientific basis for the prevention and treatment of prosthesis loosening. Zoledronic Acid 146-148 sphingosine kinase 1 Homo sapiens 130-134 35237148-16 2021 Conclusion: Our study revealed that wear particles could induce inflammatory osteolysis by upregulating SPHKs/S1P-TRAF2-BECN1 and SPHK inhibitors/ZA inhibit osteoclastogenesis in vitro and prevent inflammatory osteolysis in vivo, suggesting that SPHK inhibitors and ZA can be a new perspective and scientific basis for the prevention and treatment of prosthesis loosening. Zoledronic Acid 146-148 sphingosine kinase 1 Homo sapiens 246-250 35237148-16 2021 Conclusion: Our study revealed that wear particles could induce inflammatory osteolysis by upregulating SPHKs/S1P-TRAF2-BECN1 and SPHK inhibitors/ZA inhibit osteoclastogenesis in vitro and prevent inflammatory osteolysis in vivo, suggesting that SPHK inhibitors and ZA can be a new perspective and scientific basis for the prevention and treatment of prosthesis loosening. Zoledronic Acid 266-268 sphingosine kinase 1 Homo sapiens 130-134 35115496-6 2022 The SphK1 activator K6PC-5 or sphingosine-1-phosphate partially inhibited SKI-V-induced OS cell death. K6PC-5 20-26 sphingosine kinase 1 Homo sapiens 4-9 35094989-7 2022 In the presence of Sphingosine kinase (SphK1) inhibitor, adenosine mediated effects on ATP release were abolished. Adenosine Triphosphate 87-90 sphingosine kinase 1 Homo sapiens 39-44 34051800-0 2021 miR-19a/b-3p promotes inflammation during cerebral ischemia/reperfusion injury via SIRT1/FoxO3/SPHK1 pathway. mir-19a 0-7 sphingosine kinase 1 Homo sapiens 95-100 35491169-4 2022 Stimulation with TGFbeta increased the ALDH-positive breast CSC population via the phosphorylation of sphingosine kinase 1 (SphK1), a sphingosine-1-phosphate (S1P)-producing enzyme, and subsequent S1P-mediated S1P receptor 3 (S1PR3) activation. sphingosine 1-phosphate 134-157 sphingosine kinase 1 Homo sapiens 102-122 34051800-0 2021 miR-19a/b-3p promotes inflammation during cerebral ischemia/reperfusion injury via SIRT1/FoxO3/SPHK1 pathway. b-3p 8-12 sphingosine kinase 1 Homo sapiens 95-100 34055895-2 2021 Two sphingosine kinase isoforms, sphingosine kinase 1 (SPHK1) and sphingosine kinase 2 (SPHK2), synthesize S1P by phosphorylating sphingosine. Sphingosine 4-15 sphingosine kinase 1 Homo sapiens 33-53 34033645-6 2021 Surprisingly, the toxicity and activation of the ISR triggered by 2 independent SPHK inhibitors, SKI-II and ABC294640, the latter in clinical trials, are also observed in cells lacking SPHK1 and SPHK2. N'-((2-hydroxy-1-naphthyl)methylene)-3-(2-naphthyl)-1H-pyrazole-5-carbohydrazide 97-103 sphingosine kinase 1 Homo sapiens 80-84 34055895-2 2021 Two sphingosine kinase isoforms, sphingosine kinase 1 (SPHK1) and sphingosine kinase 2 (SPHK2), synthesize S1P by phosphorylating sphingosine. Sphingosine 4-15 sphingosine kinase 1 Homo sapiens 55-60 33919234-2 2021 These BCSC were previously reported to express key sphingolipid signaling effectors, including pro-oncogenic sphingosine kinase 1 (SphK1) and sphingosine-1-phosphate receptor 3 (S1P3). Sphingolipids 51-63 sphingosine kinase 1 Homo sapiens 131-136 33175430-4 2021 METHOD OF STUDY: Sphingosine kinase 1 (SPHK1) localization, which produces sphingosine 1-phosphate (S1P), was examined in human early follicles by immunohistochemistry. sphingosine 1-phosphate 75-98 sphingosine kinase 1 Homo sapiens 17-37 33175430-4 2021 METHOD OF STUDY: Sphingosine kinase 1 (SPHK1) localization, which produces sphingosine 1-phosphate (S1P), was examined in human early follicles by immunohistochemistry. sphingosine 1-phosphate 75-98 sphingosine kinase 1 Homo sapiens 39-44 33609744-8 2021 PI3K inhibition also affected the S1P receptors and gene expression of enzymes such as the dihydroceramide delta 4 desaturase (DEGS1) and sphingosine kinase 1 (SK1) in the de novo sphingolipid pathway. Sphingolipids 180-192 sphingosine kinase 1 Homo sapiens 138-158 33609744-8 2021 PI3K inhibition also affected the S1P receptors and gene expression of enzymes such as the dihydroceramide delta 4 desaturase (DEGS1) and sphingosine kinase 1 (SK1) in the de novo sphingolipid pathway. Sphingolipids 180-192 sphingosine kinase 1 Homo sapiens 160-163 34026822-10 2021 Results showed that DSCXQ intervention significantly reduced cerebral infarct size, ameliorated behavioral abnormality, inhibited the expression of Sphk1, S1PR1, CD62P, Bax, Cleaved Caspase-3, while increased the level of Bcl-2, and prevented neuronal apoptosis. dscxq 20-25 sphingosine kinase 1 Homo sapiens 148-153 33660008-4 2021 METHODS: Expression analysis of SPHK1 was performed on formalin-fixed paraffin-embedded tissue from 1005 ovarian cancer patients with different histological subtypes using immunohistochemistry. Formaldehyde 55-63 sphingosine kinase 1 Homo sapiens 32-37 33660008-4 2021 METHODS: Expression analysis of SPHK1 was performed on formalin-fixed paraffin-embedded tissue from 1005 ovarian cancer patients with different histological subtypes using immunohistochemistry. Paraffin 70-78 sphingosine kinase 1 Homo sapiens 32-37 33920887-0 2021 Targeting the Sphingosine Kinase/Sphingosine-1-Phosphate Signaling Axis in Drug Discovery for Cancer Therapy. sphingosine 1-phosphate 33-56 sphingosine kinase 1 Homo sapiens 14-32 33920887-5 2021 Sphingosine kinase (SphK) catalyzes the synthesis of S1P from sphingosine and modulates several cellular processes and is found to be essentially involved in various pathophysiological conditions. Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 0-18 33920887-5 2021 Sphingosine kinase (SphK) catalyzes the synthesis of S1P from sphingosine and modulates several cellular processes and is found to be essentially involved in various pathophysiological conditions. Sphingosine 62-73 sphingosine kinase 1 Homo sapiens 20-24 33865856-5 2021 In addition, carbachol and PMA induced translocation of monomeric GFP-mSK1 to lamellipodia, while S1P induced translocation of dimeric GFP-mSK1 to filopodia, suggesting that SK1 regulates different cell biological processes dependent on dimerization. Carbachol 13-22 sphingosine kinase 1 Homo sapiens 71-74 32275078-1 2021 Sphingosine kinases type 1 (SphK1) is a key enzyme in the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). Sphingosine 77-88 sphingosine kinase 1 Homo sapiens 0-26 32275078-1 2021 Sphingosine kinases type 1 (SphK1) is a key enzyme in the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). Sphingosine 77-88 sphingosine kinase 1 Homo sapiens 28-33 32275078-1 2021 Sphingosine kinases type 1 (SphK1) is a key enzyme in the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 92-115 sphingosine kinase 1 Homo sapiens 0-26 32275078-1 2021 Sphingosine kinases type 1 (SphK1) is a key enzyme in the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 92-115 sphingosine kinase 1 Homo sapiens 28-33 32275078-1 2021 Sphingosine kinases type 1 (SphK1) is a key enzyme in the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 117-120 sphingosine kinase 1 Homo sapiens 0-26 32275078-1 2021 Sphingosine kinases type 1 (SphK1) is a key enzyme in the phosphorylation of sphingosine to sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 117-120 sphingosine kinase 1 Homo sapiens 28-33 33865856-5 2021 In addition, carbachol and PMA induced translocation of monomeric GFP-mSK1 to lamellipodia, while S1P induced translocation of dimeric GFP-mSK1 to filopodia, suggesting that SK1 regulates different cell biological processes dependent on dimerization. Tetradecanoylphorbol Acetate 27-30 sphingosine kinase 1 Homo sapiens 71-74 33290840-3 2021 SK catalyzes the phosphorylation of sphingosine to S1P and exists as two isoforms, SK1 and SK2. Sphingosine 36-47 sphingosine kinase 1 Homo sapiens 83-86 33400245-11 2021 In addition, overexpression of MAFG-AS1 upregulated the expression of SphK1 in BC cells, and attenuated the inhibitory effects of miR-125b-5p on the expression of SphK1. mir-125b-5p 130-141 sphingosine kinase 1 Homo sapiens 163-168 33400245-13 2021 Moreover, overexpression of miR-125b-5p inhibited BC cell proliferation, migration, and invasion, while its effects were alleviated by overexpression of SphK1. mir-125b-5p 28-39 sphingosine kinase 1 Homo sapiens 153-158 33160044-7 2021 RESULTS: The results suggested that HCQ in the treatment of RA was mainly associated with 4 targets that are smoothened homolog (SMO), sphingosine kinase (SPHK) 1, SPHK2 and gatty-acid amide hydrolase (FAAH), with their related 3276 proteins" network which regulate ErbB, HIF-1, NF-kappaB, FoxO, chemokines, MAPK, PI3K/Akt pathways and so forth. Hydroxychloroquine 36-39 sphingosine kinase 1 Homo sapiens 135-162 33160044-11 2021 SMO, SPHK1, SPHK2 and FAAH involving 3276 proteins become the multi-targets mechanism of HCQ in the treatment of RA. Hydroxychloroquine 89-92 sphingosine kinase 1 Homo sapiens 5-10 33758708-4 2021 In this article, we review the mechanism of the sphingosine kinase/sphingosine-1-phosphate (SphK/S1P) axis along with its effect on the tumor microenvironment (TME) and compounds that have been studied inhibiting the SphK/S1P axis. sphingosine 1-phosphate 67-90 sphingosine kinase 1 Homo sapiens 92-96 33758708-8 2021 The metabolism of sphingolipids was summarized, followed by the SphK/S1P upregulation in breast cancer cells. Sphingolipids 18-31 sphingosine kinase 1 Homo sapiens 64-68 33633691-2 2020 In the sphingolipid metabolic network, ceramides serve as the central hub that is hydrolyzed to sphingosine, followed by phosphorylation to sphingosine 1-phosphate (S1P) by sphingosine kinase (SphK). Sphingolipids 7-19 sphingosine kinase 1 Homo sapiens 173-191 33797381-1 2022 BACKGROUND: SphK1 is a conserved lipid kinase, which can catalyze formation of tumor promoting factor sphingosine phosphate-1 (S1P). sphingosine phosphate 102-123 sphingosine kinase 1 Homo sapiens 12-17 33445156-5 2021 Previously, our group reported a SphK2 naphthalene-based selective inhibitor, SLC5081308, which displays approximately 7-fold selectivity for hSphK2 over hSphK1 and has a SphK2 Ki value of 1.0 muM. naphthalene 39-50 sphingosine kinase 1 Homo sapiens 154-160 33445156-5 2021 Previously, our group reported a SphK2 naphthalene-based selective inhibitor, SLC5081308, which displays approximately 7-fold selectivity for hSphK2 over hSphK1 and has a SphK2 Ki value of 1.0 muM. slc5081308 78-88 sphingosine kinase 1 Homo sapiens 154-160 33633691-2 2020 In the sphingolipid metabolic network, ceramides serve as the central hub that is hydrolyzed to sphingosine, followed by phosphorylation to sphingosine 1-phosphate (S1P) by sphingosine kinase (SphK). Sphingolipids 7-19 sphingosine kinase 1 Homo sapiens 193-197 33633691-2 2020 In the sphingolipid metabolic network, ceramides serve as the central hub that is hydrolyzed to sphingosine, followed by phosphorylation to sphingosine 1-phosphate (S1P) by sphingosine kinase (SphK). Ceramides 39-48 sphingosine kinase 1 Homo sapiens 173-191 33484475-0 2021 Sphingosine kinase 1 downregulation is required for adaptation to serine deprivation. Serine 66-72 sphingosine kinase 1 Homo sapiens 0-20 33633691-2 2020 In the sphingolipid metabolic network, ceramides serve as the central hub that is hydrolyzed to sphingosine, followed by phosphorylation to sphingosine 1-phosphate (S1P) by sphingosine kinase (SphK). Ceramides 39-48 sphingosine kinase 1 Homo sapiens 193-197 33633691-2 2020 In the sphingolipid metabolic network, ceramides serve as the central hub that is hydrolyzed to sphingosine, followed by phosphorylation to sphingosine 1-phosphate (S1P) by sphingosine kinase (SphK). Sphingosine 96-107 sphingosine kinase 1 Homo sapiens 193-197 33633691-2 2020 In the sphingolipid metabolic network, ceramides serve as the central hub that is hydrolyzed to sphingosine, followed by phosphorylation to sphingosine 1-phosphate (S1P) by sphingosine kinase (SphK). sphingosine 1-phosphate 140-163 sphingosine kinase 1 Homo sapiens 173-191 33633691-2 2020 In the sphingolipid metabolic network, ceramides serve as the central hub that is hydrolyzed to sphingosine, followed by phosphorylation to sphingosine 1-phosphate (S1P) by sphingosine kinase (SphK). sphingosine 1-phosphate 140-163 sphingosine kinase 1 Homo sapiens 193-197 33633691-3 2020 SphK is regarded as a "switch" of the sphingolipid rheostat, as it catalyzes the conversion of ceramide/sphingosine to S1P, which often exhibit opposing biological roles in the cell. Ceramides 95-103 sphingosine kinase 1 Homo sapiens 0-4 33633691-3 2020 SphK is regarded as a "switch" of the sphingolipid rheostat, as it catalyzes the conversion of ceramide/sphingosine to S1P, which often exhibit opposing biological roles in the cell. Sphingosine 104-115 sphingosine kinase 1 Homo sapiens 0-4 33565370-0 2021 Investigation of sphingosine kinase 1 inhibitory potential of cinchonine and colcemid targeting anticancer therapy. cinchonine 62-72 sphingosine kinase 1 Homo sapiens 17-37 33565370-0 2021 Investigation of sphingosine kinase 1 inhibitory potential of cinchonine and colcemid targeting anticancer therapy. Demecolcine 77-85 sphingosine kinase 1 Homo sapiens 17-37 33565370-5 2021 Here, we have investigated the binding affinity and SphK1 inhibitory potential of cinchonine and colcemid using a combined molecular docking and simulation studies followed by experimental analysis. cinchonine 82-92 sphingosine kinase 1 Homo sapiens 52-57 33565370-5 2021 Here, we have investigated the binding affinity and SphK1 inhibitory potential of cinchonine and colcemid using a combined molecular docking and simulation studies followed by experimental analysis. Demecolcine 97-105 sphingosine kinase 1 Homo sapiens 52-57 33565370-7 2021 Further, MD simulation studies revealed that both cinchonine and colcemid bind to the residues at the active site pocket of SphK1 with several non-covalent interactions, which may be responsible for inhibiting its kinase activity. cinchonine 50-60 sphingosine kinase 1 Homo sapiens 124-129 33565370-7 2021 Further, MD simulation studies revealed that both cinchonine and colcemid bind to the residues at the active site pocket of SphK1 with several non-covalent interactions, which may be responsible for inhibiting its kinase activity. Demecolcine 65-73 sphingosine kinase 1 Homo sapiens 124-129 33565370-8 2021 Besides, the binding of cinchonine and colcemid causes substantial conformational changes in the structure of SphK1. cinchonine 24-34 sphingosine kinase 1 Homo sapiens 110-115 33565370-8 2021 Besides, the binding of cinchonine and colcemid causes substantial conformational changes in the structure of SphK1. Demecolcine 39-47 sphingosine kinase 1 Homo sapiens 110-115 33565370-9 2021 Taken together, cinchonine and colcemid may be implicated in designing potential drug molecules with improved affinity and specificity for SphK1 targeting anticancer therapy. cinchonine 16-26 sphingosine kinase 1 Homo sapiens 139-144 33565370-9 2021 Taken together, cinchonine and colcemid may be implicated in designing potential drug molecules with improved affinity and specificity for SphK1 targeting anticancer therapy. Demecolcine 31-39 sphingosine kinase 1 Homo sapiens 139-144 33484475-4 2021 Previously, serine deprivation has been connected to the action of the tumor suppressor p53, and we have previously published on a role for p53 regulating sphingosine kinase 1 (SK1), an enzyme that phosphorylates sphingosine to form sphingosine-1-phosphate (S1P). Serine 12-18 sphingosine kinase 1 Homo sapiens 177-180 33484475-4 2021 Previously, serine deprivation has been connected to the action of the tumor suppressor p53, and we have previously published on a role for p53 regulating sphingosine kinase 1 (SK1), an enzyme that phosphorylates sphingosine to form sphingosine-1-phosphate (S1P). Sphingosine 155-166 sphingosine kinase 1 Homo sapiens 177-180 33484475-4 2021 Previously, serine deprivation has been connected to the action of the tumor suppressor p53, and we have previously published on a role for p53 regulating sphingosine kinase 1 (SK1), an enzyme that phosphorylates sphingosine to form sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 233-256 sphingosine kinase 1 Homo sapiens 155-175 33484475-4 2021 Previously, serine deprivation has been connected to the action of the tumor suppressor p53, and we have previously published on a role for p53 regulating sphingosine kinase 1 (SK1), an enzyme that phosphorylates sphingosine to form sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 233-256 sphingosine kinase 1 Homo sapiens 177-180 33484475-5 2021 SK1 is a key enzyme in sphingolipid synthesis that functions in pro-survival and tumor-promoting pathways and whose expression is also often elevated in cancers. Sphingolipids 23-35 sphingosine kinase 1 Homo sapiens 0-3 33484475-6 2021 Here we show that SK1 was degraded during serine starvation in a time and dose-dependent manner, which led to sphingosine accumulation. Serine 42-48 sphingosine kinase 1 Homo sapiens 18-21 33484475-6 2021 Here we show that SK1 was degraded during serine starvation in a time and dose-dependent manner, which led to sphingosine accumulation. Sphingosine 110-121 sphingosine kinase 1 Homo sapiens 18-21 33484475-8 2021 Furthermore, we show that overexpression of SK1, to compensate for SK1 loss, was detrimental to cell growth under conditions of serine starvation, demonstrating that the suppression of SK1 under these conditions is adaptive. Serine 128-134 sphingosine kinase 1 Homo sapiens 44-47 33484475-8 2021 Furthermore, we show that overexpression of SK1, to compensate for SK1 loss, was detrimental to cell growth under conditions of serine starvation, demonstrating that the suppression of SK1 under these conditions is adaptive. Serine 128-134 sphingosine kinase 1 Homo sapiens 67-70 33484475-8 2021 Furthermore, we show that overexpression of SK1, to compensate for SK1 loss, was detrimental to cell growth under conditions of serine starvation, demonstrating that the suppression of SK1 under these conditions is adaptive. Serine 128-134 sphingosine kinase 1 Homo sapiens 67-70 33484475-9 2021 Mitochondrial oxygen consumption decreased in response to SK1 degradation, and this was accompanied by an increase in intracellular reactive oxygen species (ROS). Oxygen 14-20 sphingosine kinase 1 Homo sapiens 58-61 33484475-11 2021 The effects of SK1 suppression on ROS were mimicked by D-erythro-sphingosine, whereas S1P was ineffective, suggesting that the effects of loss of SK1 were due to the accumulation of its substrate sphingosine. Sphingosine 55-76 sphingosine kinase 1 Homo sapiens 15-18 33484475-11 2021 The effects of SK1 suppression on ROS were mimicked by D-erythro-sphingosine, whereas S1P was ineffective, suggesting that the effects of loss of SK1 were due to the accumulation of its substrate sphingosine. Sphingosine 65-76 sphingosine kinase 1 Homo sapiens 15-18 33484475-12 2021 This study reveals a new mechanism for regulating SK1 levels and a link of SK1 to serine starvation as well as mitochondrial function. Serine 82-88 sphingosine kinase 1 Homo sapiens 75-78 33414460-0 2021 Targeting sphingosine kinase 1 (SK1) enhances oncogene-induced senescence through ceramide synthase 2 (CerS2)-mediated generation of very-long-chain ceramides. Ceramides 149-158 sphingosine kinase 1 Homo sapiens 10-30 33385956-6 2021 Molecular modeling studies of 22d docked inside the Sph binding pocket of both SphK1 and SphK2 indicate essential hydrogen bond between the 2-(hydroxymethyl)pyrrolidine head to interact with aspartic acid and serine residues near the ATP binding pocket, which provide the basis for dual inhibition. Serine 209-215 sphingosine kinase 1 Homo sapiens 79-84 33385956-6 2021 Molecular modeling studies of 22d docked inside the Sph binding pocket of both SphK1 and SphK2 indicate essential hydrogen bond between the 2-(hydroxymethyl)pyrrolidine head to interact with aspartic acid and serine residues near the ATP binding pocket, which provide the basis for dual inhibition. Adenosine Triphosphate 234-237 sphingosine kinase 1 Homo sapiens 79-84 33385956-7 2021 In addition, the dodecyl tail adopts a "J-shape" conformation found in crystal structure of sphingosine bound to SphK1. Sphingosine 92-103 sphingosine kinase 1 Homo sapiens 113-118 33441957-3 2021 Erythrocyte possesses Sphingosine kinase (SphK) that catalyzed its biosynthesis from sphingosine (Sph). Sphingosine 85-96 sphingosine kinase 1 Homo sapiens 42-46 33441957-3 2021 Erythrocyte possesses Sphingosine kinase (SphK) that catalyzed its biosynthesis from sphingosine (Sph). Sphingosine 22-25 sphingosine kinase 1 Homo sapiens 42-46 33441957-6 2021 Next, by targeting of the SphK through specific inhibitor N,N-Dimethylsphingosine DMS, we show a reduction in erythrocyte endogenous S1P pool and SphK-phosphorylation that led to inhibition in growth and development of ring stage P. falciparum. n,n-dimethylsphingosine dms 58-85 sphingosine kinase 1 Homo sapiens 26-30 33441957-6 2021 Next, by targeting of the SphK through specific inhibitor N,N-Dimethylsphingosine DMS, we show a reduction in erythrocyte endogenous S1P pool and SphK-phosphorylation that led to inhibition in growth and development of ring stage P. falciparum. n,n-dimethylsphingosine dms 58-85 sphingosine kinase 1 Homo sapiens 146-150 33414460-0 2021 Targeting sphingosine kinase 1 (SK1) enhances oncogene-induced senescence through ceramide synthase 2 (CerS2)-mediated generation of very-long-chain ceramides. Ceramides 149-158 sphingosine kinase 1 Homo sapiens 32-35 33414460-5 2021 Since Cer and sphingosine-1-phosphate have been shown to exert opposite effects on cellular senescence, we hypothesized that targeting SK1 could enhance oncogenic K-Ras-induced senescence. sphingosine 1-phosphate 14-37 sphingosine kinase 1 Homo sapiens 135-138 33414460-6 2021 Indeed, SK1 downregulation or inhibition enhanced p21 expression and SA-beta-gal in cells expressing oncogenic K-Ras and impeded cell growth. 2-(2-quinolinyl)-1H-indene--1,3(2H)-dione-6'-sulfonic acid 69-80 sphingosine kinase 1 Homo sapiens 8-11 33406544-0 2020 [The study of the protection function of the sphingosine kinase 1 in the nerve cell damage caused by acrylamide]. Acrylamide 101-111 sphingosine kinase 1 Homo sapiens 45-65 33414358-1 2020 MPP+ (1-methyl-4-phenylpyridinium)-induced dopaminergic neuronal cell apoptosis is associated with sphingosine kinase 1 (SphK1) inhibition. 1-Methyl-4-phenylpyridinium 6-33 sphingosine kinase 1 Homo sapiens 99-119 33414358-1 2020 MPP+ (1-methyl-4-phenylpyridinium)-induced dopaminergic neuronal cell apoptosis is associated with sphingosine kinase 1 (SphK1) inhibition. 1-Methyl-4-phenylpyridinium 6-33 sphingosine kinase 1 Homo sapiens 121-126 33414358-6 2020 miR-6862 inhibition exerted opposite activity and elevated SphK1 expression. mir-6862 0-8 sphingosine kinase 1 Homo sapiens 59-64 33414358-10 2020 These results suggest that inhibition of miR-6862 induces SphK1 elevation and protects neuronal cells from MPP+-induced cell death. mir-6862 41-49 sphingosine kinase 1 Homo sapiens 58-63 33406544-1 2020 Objective: To study the protective effect and effect of SphK1 overexpression on the injury of nerve cells induced by acrylamide. Acrylamide 117-127 sphingosine kinase 1 Homo sapiens 56-61 33406544-13 2020 Conclusion: The SphK1 excessive expression plays the protective function to the nerve cell damage caused by acrylamide. Acrylamide 108-118 sphingosine kinase 1 Homo sapiens 16-21 33334894-2 2021 This potent lipid mediator is synthesized from the sphingosine precursor by two sphingosine kinases (SphK1 and 2) and must be exported to exert extracellular signalling functions. Sphingosine 51-62 sphingosine kinase 1 Homo sapiens 101-112 33334894-6 2021 The uptake of sphingosine in erythrocytes is facilitated by the activity of SphK1. Sphingosine 14-25 sphingosine kinase 1 Homo sapiens 76-81 33334894-7 2021 By converting sphingosine into S1P, SphK1 indirectly increases the influx of sphingosine, a process that is irreversible in erythrocytes. Sphingosine 14-25 sphingosine kinase 1 Homo sapiens 36-41 33334894-7 2021 By converting sphingosine into S1P, SphK1 indirectly increases the influx of sphingosine, a process that is irreversible in erythrocytes. Sphingosine 77-88 sphingosine kinase 1 Homo sapiens 36-41 32771459-1 2020 Phosphorylation of sphingosine by sphingosine kinase 1 (SPHK1) produces the bioactive sphingolipid sphingosine-1-phosphate (S1P), a microvascular and immuno-modulator associated with vascular remodeling in pulmonary arterial hypertension (PAH). Sphingosine 19-30 sphingosine kinase 1 Homo sapiens 34-54 33385956-0 2021 Lipophilic tail modifications of 2-(hydroxymethyl)pyrrolidine scaffold reveal dual sphingosine kinase 1 and 2 inhibitors. prolinol 33-61 sphingosine kinase 1 Homo sapiens 83-109 33385956-2 2021 The synthesis of S1P is catalyzed by the action of sphingosine kinase 1 or 2 (SphK1 or SphK2) on sphingosine and ATP. Sphingosine 51-62 sphingosine kinase 1 Homo sapiens 78-83 33385956-2 2021 The synthesis of S1P is catalyzed by the action of sphingosine kinase 1 or 2 (SphK1 or SphK2) on sphingosine and ATP. Adenosine Triphosphate 113-116 sphingosine kinase 1 Homo sapiens 51-76 33385956-2 2021 The synthesis of S1P is catalyzed by the action of sphingosine kinase 1 or 2 (SphK1 or SphK2) on sphingosine and ATP. Adenosine Triphosphate 113-116 sphingosine kinase 1 Homo sapiens 78-83 33385956-4 2021 Towards this end, we report the structure-activity relationship profiling of 2-(hydroxymethyl)pyrrolidine-based inhibitors with 22d being the most potent dual SphK1/SphK2 inhibitor (SphK1 Ki = 0.679 muM, SphK2 Ki = 0.951 muM) reported in this series. prolinol 77-105 sphingosine kinase 1 Homo sapiens 159-164 33385956-6 2021 Molecular modeling studies of 22d docked inside the Sph binding pocket of both SphK1 and SphK2 indicate essential hydrogen bond between the 2-(hydroxymethyl)pyrrolidine head to interact with aspartic acid and serine residues near the ATP binding pocket, which provide the basis for dual inhibition. Hydrogen 114-122 sphingosine kinase 1 Homo sapiens 79-84 33385956-6 2021 Molecular modeling studies of 22d docked inside the Sph binding pocket of both SphK1 and SphK2 indicate essential hydrogen bond between the 2-(hydroxymethyl)pyrrolidine head to interact with aspartic acid and serine residues near the ATP binding pocket, which provide the basis for dual inhibition. prolinol 140-168 sphingosine kinase 1 Homo sapiens 79-84 33385956-6 2021 Molecular modeling studies of 22d docked inside the Sph binding pocket of both SphK1 and SphK2 indicate essential hydrogen bond between the 2-(hydroxymethyl)pyrrolidine head to interact with aspartic acid and serine residues near the ATP binding pocket, which provide the basis for dual inhibition. Aspartic Acid 191-204 sphingosine kinase 1 Homo sapiens 79-84 33035646-1 2020 The sphingosine kinases, SK1 and SK2, catalyse the formation of the bioactive signalling lipid, sphingosine 1-phosphate (S1P), from sphingosine. sphingosine 1-phosphate 96-119 sphingosine kinase 1 Homo sapiens 25-28 33035646-1 2020 The sphingosine kinases, SK1 and SK2, catalyse the formation of the bioactive signalling lipid, sphingosine 1-phosphate (S1P), from sphingosine. sphingosine 1-phosphate 121-124 sphingosine kinase 1 Homo sapiens 25-28 33035646-1 2020 The sphingosine kinases, SK1 and SK2, catalyse the formation of the bioactive signalling lipid, sphingosine 1-phosphate (S1P), from sphingosine. Sphingosine 4-15 sphingosine kinase 1 Homo sapiens 25-28 33035646-6 2020 Our analysis accounts for the contrasting behaviour of fingolimod and safingol as non-turnover inhibitors of SK1, but substrates for SK2, and the observed stereoselectivity for phosphorylation of the pro-S hydroxymethyl group of fingolimod to generate (S)-FTY720-P in vivo. Fingolimod Hydrochloride 55-65 sphingosine kinase 1 Homo sapiens 109-112 33035646-6 2020 Our analysis accounts for the contrasting behaviour of fingolimod and safingol as non-turnover inhibitors of SK1, but substrates for SK2, and the observed stereoselectivity for phosphorylation of the pro-S hydroxymethyl group of fingolimod to generate (S)-FTY720-P in vivo. safingol 70-78 sphingosine kinase 1 Homo sapiens 109-112 33035646-10 2020 Similarly, the biological role of sphingadiene 1-phosphates derived from plant-based dietary sphingadienes, which we also show here are substrates for both SK1 and SK2, merits investigation. sphingadiene 1-phosphates 34-59 sphingosine kinase 1 Homo sapiens 156-159 33035646-10 2020 Similarly, the biological role of sphingadiene 1-phosphates derived from plant-based dietary sphingadienes, which we also show here are substrates for both SK1 and SK2, merits investigation. sphingadienes 93-106 sphingosine kinase 1 Homo sapiens 156-159 32949605-10 2020 In addition, suppression of S1P synthesis by Sphk1 inhibitor PF-543 reduced the expression of cPLA2 and cell apoptosis but at the same time restored the normal level of UCN in cell culture supernatant. PF-543 61-67 sphingosine kinase 1 Homo sapiens 45-50 33154352-6 2020 In NSCLC cells GDC-0349 induced sphingosine kinase 1 (SphK1) inhibition, ceramide accumulation, JNK activation and oxidative injury. GDC-0349 15-23 sphingosine kinase 1 Homo sapiens 32-52 33154352-6 2020 In NSCLC cells GDC-0349 induced sphingosine kinase 1 (SphK1) inhibition, ceramide accumulation, JNK activation and oxidative injury. GDC-0349 15-23 sphingosine kinase 1 Homo sapiens 54-59 33154352-9 2020 Akt-mTOR in-activation, SphK1 inhibition, JNK activation and oxidative stress were detected in NSCLC xenograft tissues with GDC-0349 administration. GDC-0349 124-132 sphingosine kinase 1 Homo sapiens 24-29 33065444-1 2020 IMMH001, which is a prodrug for sphingosine-1-phosphate receptor 1 (S1P1) agonist, is converted to the active form, its monophosphate ester (S)-IMMH001-P, by sphingosine kinase 1 (SphK1) and sphingosine kinase 2 (SphK2) in vivo. monophosphate ester 120-139 sphingosine kinase 1 Homo sapiens 158-178 33065444-1 2020 IMMH001, which is a prodrug for sphingosine-1-phosphate receptor 1 (S1P1) agonist, is converted to the active form, its monophosphate ester (S)-IMMH001-P, by sphingosine kinase 1 (SphK1) and sphingosine kinase 2 (SphK2) in vivo. monophosphate ester 120-139 sphingosine kinase 1 Homo sapiens 180-185 31752564-6 2020 Finally, it was observed that the ZINC06823429 binds tightly to catalytic site of SphK1 and remain stable during MD simulations. zinc06823429 34-46 sphingosine kinase 1 Homo sapiens 82-87 32919113-1 2020 Sphingosine kinase (SphK) is primarily responsible for the production of Sphingosine-1-phosphate (S1P) that plays an important role in many biological and pathobiological processes including cancer, inflammation, neurological and cardiovascular disorders. sphingosine 1-phosphate 73-96 sphingosine kinase 1 Homo sapiens 0-18 32919113-1 2020 Sphingosine kinase (SphK) is primarily responsible for the production of Sphingosine-1-phosphate (S1P) that plays an important role in many biological and pathobiological processes including cancer, inflammation, neurological and cardiovascular disorders. sphingosine 1-phosphate 73-96 sphingosine kinase 1 Homo sapiens 20-24 32919113-1 2020 Sphingosine kinase (SphK) is primarily responsible for the production of Sphingosine-1-phosphate (S1P) that plays an important role in many biological and pathobiological processes including cancer, inflammation, neurological and cardiovascular disorders. sphingosine 1-phosphate 98-101 sphingosine kinase 1 Homo sapiens 0-18 32919113-1 2020 Sphingosine kinase (SphK) is primarily responsible for the production of Sphingosine-1-phosphate (S1P) that plays an important role in many biological and pathobiological processes including cancer, inflammation, neurological and cardiovascular disorders. sphingosine 1-phosphate 98-101 sphingosine kinase 1 Homo sapiens 20-24 33171624-2 2020 Sphingosine kinase 1, or SK1, is one of two sphingosine kinases that phosphorylate sphingosine into sphingosine-1-phosphate (S1P). Sphingosine 44-55 sphingosine kinase 1 Homo sapiens 0-20 33171624-2 2020 Sphingosine kinase 1, or SK1, is one of two sphingosine kinases that phosphorylate sphingosine into sphingosine-1-phosphate (S1P). Sphingosine 44-55 sphingosine kinase 1 Homo sapiens 25-28 33171624-2 2020 Sphingosine kinase 1, or SK1, is one of two sphingosine kinases that phosphorylate sphingosine into sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 100-123 sphingosine kinase 1 Homo sapiens 0-20 33171624-2 2020 Sphingosine kinase 1, or SK1, is one of two sphingosine kinases that phosphorylate sphingosine into sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 100-123 sphingosine kinase 1 Homo sapiens 25-28 33171624-2 2020 Sphingosine kinase 1, or SK1, is one of two sphingosine kinases that phosphorylate sphingosine into sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 125-128 sphingosine kinase 1 Homo sapiens 0-20 33171624-2 2020 Sphingosine kinase 1, or SK1, is one of two sphingosine kinases that phosphorylate sphingosine into sphingosine-1-phosphate (S1P). sphingosine 1-phosphate 125-128 sphingosine kinase 1 Homo sapiens 25-28 33139717-5 2020 RSV was able to inhibit lipopolysaccharide (LPS) stimulated inflammatory responses through blocking Phospholipase D (PLD) and its downstream signaling molecules SphK1, ERK1/2 and NF-kappaB. Resveratrol 0-3 sphingosine kinase 1 Homo sapiens 161-166 32771459-1 2020 Phosphorylation of sphingosine by sphingosine kinase 1 (SPHK1) produces the bioactive sphingolipid sphingosine-1-phosphate (S1P), a microvascular and immuno-modulator associated with vascular remodeling in pulmonary arterial hypertension (PAH). Sphingosine 19-30 sphingosine kinase 1 Homo sapiens 56-61 32771459-1 2020 Phosphorylation of sphingosine by sphingosine kinase 1 (SPHK1) produces the bioactive sphingolipid sphingosine-1-phosphate (S1P), a microvascular and immuno-modulator associated with vascular remodeling in pulmonary arterial hypertension (PAH). Sphingolipids 86-98 sphingosine kinase 1 Homo sapiens 34-54 32771459-1 2020 Phosphorylation of sphingosine by sphingosine kinase 1 (SPHK1) produces the bioactive sphingolipid sphingosine-1-phosphate (S1P), a microvascular and immuno-modulator associated with vascular remodeling in pulmonary arterial hypertension (PAH). Sphingolipids 86-98 sphingosine kinase 1 Homo sapiens 56-61 32771459-1 2020 Phosphorylation of sphingosine by sphingosine kinase 1 (SPHK1) produces the bioactive sphingolipid sphingosine-1-phosphate (S1P), a microvascular and immuno-modulator associated with vascular remodeling in pulmonary arterial hypertension (PAH). sphingosine 1-phosphate 99-122 sphingosine kinase 1 Homo sapiens 34-54 32771459-1 2020 Phosphorylation of sphingosine by sphingosine kinase 1 (SPHK1) produces the bioactive sphingolipid sphingosine-1-phosphate (S1P), a microvascular and immuno-modulator associated with vascular remodeling in pulmonary arterial hypertension (PAH). sphingosine 1-phosphate 99-122 sphingosine kinase 1 Homo sapiens 56-61 32771517-0 2020 Mechanistic insights into the urea-induced denaturation of human sphingosine kinase 1. Urea 30-34 sphingosine kinase 1 Homo sapiens 65-85 32771517-3 2020 In the current work, the urea-induced unfolding of SphK1 was performed at pH 8.0 and 25 C using CD and fluorescence spectroscopy. Urea 25-29 sphingosine kinase 1 Homo sapiens 51-56 32771517-3 2020 In the current work, the urea-induced unfolding of SphK1 was performed at pH 8.0 and 25 C using CD and fluorescence spectroscopy. Cadmium 97-99 sphingosine kinase 1 Homo sapiens 51-56 32771517-4 2020 SphK1 follows a biphasic unfolding transition (N I D) with an intermediate (I) state populated around 4.0 M urea concentration in the presence of urea. Urea 112-116 sphingosine kinase 1 Homo sapiens 0-5 32771517-4 2020 SphK1 follows a biphasic unfolding transition (N I D) with an intermediate (I) state populated around 4.0 M urea concentration in the presence of urea. Urea 150-154 sphingosine kinase 1 Homo sapiens 0-5 32771517-5 2020 The circular dichroism ([theta]222) and fluorescence emission spectra (lambdamax) of SphK1 with increasing concentrations of urea were analyzed to calculate Gibbs free energy (DeltaG0) for both the transitions (N I and I D). Urea 125-129 sphingosine kinase 1 Homo sapiens 85-90 32771517-7 2020 Also, we performed 100 ns molecular dynamics (MD) simulations to get atomistic insights into the structural changes in SphK1 with increasing urea concentrations. Urea 141-145 sphingosine kinase 1 Homo sapiens 119-124 32771517-8 2020 Our results showed a consistent pattern of the SphK1 unfolding with increasing urea concentrations. Urea 79-83 sphingosine kinase 1 Homo sapiens 47-52 31985036-6 2020 The results demonstrated that after exposure to hydrogen peroxide, cell viability was reduced whereas SPHK1 expression was significantly elevated. Hydrogen Peroxide 48-65 sphingosine kinase 1 Homo sapiens 102-107 33123153-1 2020 Sphingosine kinase 1 (SPHK1) is a crucial molecule that catalyzes sphingosine to synthesize sphingosine-1-phosphate (S1P), facilitating cell survival signaling. Sphingosine 66-77 sphingosine kinase 1 Homo sapiens 0-20 33123153-1 2020 Sphingosine kinase 1 (SPHK1) is a crucial molecule that catalyzes sphingosine to synthesize sphingosine-1-phosphate (S1P), facilitating cell survival signaling. Sphingosine 66-77 sphingosine kinase 1 Homo sapiens 22-27 33123153-1 2020 Sphingosine kinase 1 (SPHK1) is a crucial molecule that catalyzes sphingosine to synthesize sphingosine-1-phosphate (S1P), facilitating cell survival signaling. sphingosine 1-phosphate 92-115 sphingosine kinase 1 Homo sapiens 0-20 33123153-1 2020 Sphingosine kinase 1 (SPHK1) is a crucial molecule that catalyzes sphingosine to synthesize sphingosine-1-phosphate (S1P), facilitating cell survival signaling. sphingosine 1-phosphate 92-115 sphingosine kinase 1 Homo sapiens 22-27 33123153-1 2020 Sphingosine kinase 1 (SPHK1) is a crucial molecule that catalyzes sphingosine to synthesize sphingosine-1-phosphate (S1P), facilitating cell survival signaling. sphingosine 1-phosphate 117-120 sphingosine kinase 1 Homo sapiens 0-20 33123153-1 2020 Sphingosine kinase 1 (SPHK1) is a crucial molecule that catalyzes sphingosine to synthesize sphingosine-1-phosphate (S1P), facilitating cell survival signaling. sphingosine 1-phosphate 117-120 sphingosine kinase 1 Homo sapiens 22-27 33123153-9 2020 Second, it could restrain SPHK1 translocation from the cytoplasm to the plasma membrane and further pyroptosis, which not only drive M2 macrophages death but also facilitate tumor microenvironment inflammation as well as the further release of sphingosine from damaged macrophages. Sphingosine 244-255 sphingosine kinase 1 Homo sapiens 26-31 32690594-2 2020 Sphingosine kinase 1 (SPHK1), which generates sphingosine-1-phosphate, has been shown to increase in liver and adipose of obese humans and mice and to regulate inflammation in hepatocytes and adipose tissue, insulin resistance, and systemic inflammation in mouse models of obesity. sphingosine 1-phosphate 46-69 sphingosine kinase 1 Homo sapiens 0-20 32690594-2 2020 Sphingosine kinase 1 (SPHK1), which generates sphingosine-1-phosphate, has been shown to increase in liver and adipose of obese humans and mice and to regulate inflammation in hepatocytes and adipose tissue, insulin resistance, and systemic inflammation in mouse models of obesity. sphingosine 1-phosphate 46-69 sphingosine kinase 1 Homo sapiens 22-27 31985036-7 2020 Knockdown of SPHK1 attenuated hydrogen peroxide-mediated cell death and reversed cell viability. Hydrogen Peroxide 30-47 sphingosine kinase 1 Homo sapiens 13-18 31985036-8 2020 Our data also demonstrated that SPHK1 deletion reduced endoplasm reticulum stress and alleviated inflammation response in hydrogen peroxide-treated cells. Hydrogen Peroxide 122-139 sphingosine kinase 1 Homo sapiens 32-37 31985036-11 2020 Altogether, our results demonstrated that SPHK1 upregulation, induced by hydrogen peroxide, is responsible for cerebral IR injury through inducing endoplasm reticulum stress and inflammation response in a manner working through the NF-kappaB signaling pathway. Hydrogen Peroxide 73-90 sphingosine kinase 1 Homo sapiens 42-47 33003441-1 2020 Diverse extracellular signals induce plasma membrane translocation of sphingosine kinase-1 (SphK1), thereby enabling inside-out signaling of sphingosine-1-phosphate. sphingosine 1-phosphate 141-164 sphingosine kinase 1 Homo sapiens 70-90 33003441-1 2020 Diverse extracellular signals induce plasma membrane translocation of sphingosine kinase-1 (SphK1), thereby enabling inside-out signaling of sphingosine-1-phosphate. sphingosine 1-phosphate 141-164 sphingosine kinase 1 Homo sapiens 92-97 33003441-2 2020 We have shown before that Gq-coupled receptors and constitutively active Galphaq/11 specifically induced a rapid and long-lasting SphK1 translocation, independently of canonical Gq/phospholipase C (PLC) signaling. galphaq 73-80 sphingosine kinase 1 Homo sapiens 130-135 33003441-5 2020 Both wild-type SphK1 and catalytically inactive SphK1-G82D reduced M3 receptor-stimulated inositol phosphate production, suggesting competition at Galphaq. Inositol Phosphates 90-108 sphingosine kinase 1 Homo sapiens 48-53 33003441-5 2020 Both wild-type SphK1 and catalytically inactive SphK1-G82D reduced M3 receptor-stimulated inositol phosphate production, suggesting competition at Galphaq. galphaq 147-154 sphingosine kinase 1 Homo sapiens 48-53 33003441-7 2020 Finally, an AIXXPL motif was identified in vertebrate SphK1 (positions 100-105 in human SphK1a), which resembles the Galphaq binding motif, ALXXPI, in PLCbeta and p63RhoGEF. galphaq 117-124 sphingosine kinase 1 Homo sapiens 54-59 33463106-0 2020 Leucine-rich Repeats and Immunoglobulin 1 (LRIG1) Ameliorates Liver Fibrosis and Hepatic Stellate Cell Activation via Inhibiting Sphingosine Kinase 1 (SphK1)/Sphingosine-1-Phosphate (S1P) Pathway. sphingosine 1-phosphate 158-181 sphingosine kinase 1 Homo sapiens 151-156 32569675-1 2020 As SET protein is overexpressed and PP2A activity is reduced in oral squamous cell carcinoma (OSCC), this study aimed to assess the effects induced by OP449, a PP2A activator/SET inhibitor, on OSCC cells in vitro, and its potential either isolated or combined with FTY720, a PP2A activator/sphingosine kinase 1 antagonist, as antitumoral therapy in vivo. op449 151-156 sphingosine kinase 1 Homo sapiens 290-310 32697651-11 2020 In vitro studies using human primary airway epithelial cells (HAEpCs) showed that SPHK1 inhibition or deletion restored HO-induced reduction in E-cadherin and reduced formation of mitochondrial reactive oxygen species (mtROS). Reactive Oxygen Species 194-217 sphingosine kinase 1 Homo sapiens 82-87 32634578-0 2020 Treatment with K6PC-5, a selective stimulator of SPHK1, ameliorates intestinal homeostasis in an animal model of Huntington"s disease. K6PC-5 15-21 sphingosine kinase 1 Homo sapiens 49-54 32705189-0 2020 Sphingosine kinase 1 contributes to doxorubicin resistance and glycolysis in osteosarcoma. Doxorubicin 36-47 sphingosine kinase 1 Homo sapiens 0-20 32705189-7 2020 SphK1 knockdown or overexpression altered doxorubicin resistance and the viability of OS cell lines. Doxorubicin 42-53 sphingosine kinase 1 Homo sapiens 0-5 32705189-8 2020 In addition, hypoxia inducible factor-1alpha (HIF-1alpha) expression was positively associated with SphK1 expression, and partly mediated SphK1-induced effects on doxorubicin resistance and glycolysis. Doxorubicin 163-174 sphingosine kinase 1 Homo sapiens 138-143 32705189-9 2020 The present study suggested that SphK1 participated in the development of doxorubicin resistance and contributed to glycolysis in OS cells by regulating HIF-1alpha expression. Doxorubicin 74-85 sphingosine kinase 1 Homo sapiens 33-38 32780686-10 2020 Conclusions: E2F1 worked as a TF of SPHK1 and exhibited anti-inflammatory and antioxidative effects through SPHK1 in PMA-induced HaCaT cells after 625 nm PBM. Tetradecanoylphorbol Acetate 117-120 sphingosine kinase 1 Homo sapiens 108-113 32780686-0 2020 Role of E2F1/SPHK1 and HSP27 During Irradiation in a PMA-Induced Inflammatory Model. Tetradecanoylphorbol Acetate 53-56 sphingosine kinase 1 Homo sapiens 13-18 32780686-6 2020 Results: A total of 6 TFs (e.g., E2F1) and 51 key DEGs (e.g., SPHK1) were identified after 625 nm PBM in PMA-stimulated HaCaT cells. pbm 98-101 sphingosine kinase 1 Homo sapiens 62-67 32780686-6 2020 Results: A total of 6 TFs (e.g., E2F1) and 51 key DEGs (e.g., SPHK1) were identified after 625 nm PBM in PMA-stimulated HaCaT cells. Tetradecanoylphorbol Acetate 105-108 sphingosine kinase 1 Homo sapiens 62-67 32905276-0 2020 Discovery of Harmaline as a Potent Inhibitor of Sphingosine Kinase-1: A Chemopreventive Role in Lung Cancer. Harmaline 13-22 sphingosine kinase 1 Homo sapiens 48-68 32905276-1 2020 The sphingosine kinase-1/sphingosine-1-phosphate pathway is linked with the cancer progression and survival of the chemotherapy-challenged cells. Phosphates 38-48 sphingosine kinase 1 Homo sapiens 4-24 32905276-3 2020 In this study, we have chosen harmaline, one of the beta-carboline alkaloids, and report its mechanism of binding to SphK1 and subsequent inhibition. Harmaline 30-39 sphingosine kinase 1 Homo sapiens 117-122 32905276-3 2020 In this study, we have chosen harmaline, one of the beta-carboline alkaloids, and report its mechanism of binding to SphK1 and subsequent inhibition. norharman 52-66 sphingosine kinase 1 Homo sapiens 117-122 32905276-4 2020 Molecular docking combined with fluorescence binding studies revealed that harmaline binds to the substrate-binding pocket of SphK1 with an appreciable binding affinity and significantly inhibits the kinase activity of SphK1 with an IC50 value in the micromolar range. Harmaline 75-84 sphingosine kinase 1 Homo sapiens 126-131 32905276-4 2020 Molecular docking combined with fluorescence binding studies revealed that harmaline binds to the substrate-binding pocket of SphK1 with an appreciable binding affinity and significantly inhibits the kinase activity of SphK1 with an IC50 value in the micromolar range. Harmaline 75-84 sphingosine kinase 1 Homo sapiens 219-224 32905276-7 2020 Our findings suggest that harmaline could be implicated as a scaffold for designing potent anticancer molecules with SphK1 inhibitory potential. Harmaline 26-35 sphingosine kinase 1 Homo sapiens 117-122 32385114-0 2020 Targeting defective sphingosine kinase 1 in Niemann-Pick type C disease with an activator mitigates cholesterol accumulation. Cholesterol 100-111 sphingosine kinase 1 Homo sapiens 20-40 32385114-4 2020 In this work, we examined the cause for sphingosine and sphingolipid accumulation in multiple cellular models of NPC disease and observed that the activity of sphingosine kinase 1 (SphK1), one of the two isoenzymes that phosphorylate sphingoid bases, was markedly reduced in both NPC1 mutant and NPC1 knockout cells. Sphingosine 40-51 sphingosine kinase 1 Homo sapiens 159-179 32385114-4 2020 In this work, we examined the cause for sphingosine and sphingolipid accumulation in multiple cellular models of NPC disease and observed that the activity of sphingosine kinase 1 (SphK1), one of the two isoenzymes that phosphorylate sphingoid bases, was markedly reduced in both NPC1 mutant and NPC1 knockout cells. Sphingosine 40-51 sphingosine kinase 1 Homo sapiens 181-186 32385114-5 2020 Conversely, SphK1 inhibition with the isotype-specific inhibitor SK1-I in wild type cells induced accumulation of cholesterol and reduced cholesterol esterification. Cholesterol 114-125 sphingosine kinase 1 Homo sapiens 12-17 32385114-5 2020 Conversely, SphK1 inhibition with the isotype-specific inhibitor SK1-I in wild type cells induced accumulation of cholesterol and reduced cholesterol esterification. Cholesterol 138-149 sphingosine kinase 1 Homo sapiens 12-17 32385114-6 2020 Of note, a novel SphK1 activator (SK1-A) that we have characterized decreased sphingoid base and complex sphingolipid accumulation and ameliorated autophagic defects in both NPC1 mutant and NPC1 knockout cells. sphingoid base 78-92 sphingosine kinase 1 Homo sapiens 17-22 32385114-8 2020 Our results indicate that a SphK1 activator rescues aberrant cholesterol and sphingolipid storage and trafficking in NPC1 mutant cells. Cholesterol 61-72 sphingosine kinase 1 Homo sapiens 28-33 32385114-8 2020 Our results indicate that a SphK1 activator rescues aberrant cholesterol and sphingolipid storage and trafficking in NPC1 mutant cells. Sphingolipids 77-89 sphingosine kinase 1 Homo sapiens 28-33 32385114-9 2020 These observations highlight a previously unknown link between SphK1 activity, NPC1, and cholesterol trafficking and metabolism. Cholesterol 89-100 sphingosine kinase 1 Homo sapiens 63-68 32244061-1 2020 Sphingosine kinases (SphK) catalyse the formation of sphingosine-1-phosphate (S1P) and play important roles in the cardiovascular, nervous and immune systems. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 0-19 32244061-1 2020 Sphingosine kinases (SphK) catalyse the formation of sphingosine-1-phosphate (S1P) and play important roles in the cardiovascular, nervous and immune systems. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 21-25 32244061-1 2020 Sphingosine kinases (SphK) catalyse the formation of sphingosine-1-phosphate (S1P) and play important roles in the cardiovascular, nervous and immune systems. sphingosine 1-phosphate 78-81 sphingosine kinase 1 Homo sapiens 0-19 32244061-1 2020 Sphingosine kinases (SphK) catalyse the formation of sphingosine-1-phosphate (S1P) and play important roles in the cardiovascular, nervous and immune systems. sphingosine 1-phosphate 78-81 sphingosine kinase 1 Homo sapiens 21-25 32780686-12 2020 Therefore, E2F1/SPHK1 and HSP27 could be used as potential biomarkers for anti-inflammatory therapy with 625 nm PBM. pbm 112-115 sphingosine kinase 1 Homo sapiens 16-21 32596609-1 2020 Sphingosine kinase 1 (SphK1) is an oncogenic lipid kinase that catalyzes the formation of sphingosine-1-phosphate via phosphorylation of sphingosine and known to play a crucial role in angiogenesis, lymphocyte trafficking, signal transduction pathways, and response to apoptotic stimuli. sphingosine 1-phosphate 90-113 sphingosine kinase 1 Homo sapiens 0-20 32006664-0 2020 Sphingosine kinase 1 is required for myristate-induced TNFalpha expression in intestinal epithelial cells. Myristates 37-46 sphingosine kinase 1 Homo sapiens 0-20 32006664-3 2020 Also, our previous studies have implicated sphingosine kinase 1 (SK1) and its product sphingosine-1-phosphate (S1P) as having key roles in the regulation of inflammatory processes in the intestinal epithelium. sphingosine 1-phosphate 86-109 sphingosine kinase 1 Homo sapiens 43-63 32006664-3 2020 Also, our previous studies have implicated sphingosine kinase 1 (SK1) and its product sphingosine-1-phosphate (S1P) as having key roles in the regulation of inflammatory processes in the intestinal epithelium. sphingosine 1-phosphate 86-109 sphingosine kinase 1 Homo sapiens 65-68 32006664-3 2020 Also, our previous studies have implicated sphingosine kinase 1 (SK1) and its product sphingosine-1-phosphate (S1P) as having key roles in the regulation of inflammatory processes in the intestinal epithelium. sphingosine 1-phosphate 111-114 sphingosine kinase 1 Homo sapiens 43-63 32006664-3 2020 Also, our previous studies have implicated sphingosine kinase 1 (SK1) and its product sphingosine-1-phosphate (S1P) as having key roles in the regulation of inflammatory processes in the intestinal epithelium. sphingosine 1-phosphate 111-114 sphingosine kinase 1 Homo sapiens 65-68 32006664-5 2020 Myristate, but not palmitate, significantly induced pro-inflammatory cytokine tumor necrosis factor alpha (TNFalpha), and it was SK1-dependent. Myristates 0-9 sphingosine kinase 1 Homo sapiens 129-132 32006664-7 2020 Additionally, myristate regulated the expression of TNFalpha via JNK activation in an SK1-dependent manner, suggesting a novel S1PR-independent target as a mediator between SK1 and JNK in response to myristate. Myristates 14-23 sphingosine kinase 1 Homo sapiens 86-89 32006664-7 2020 Additionally, myristate regulated the expression of TNFalpha via JNK activation in an SK1-dependent manner, suggesting a novel S1PR-independent target as a mediator between SK1 and JNK in response to myristate. Myristates 14-23 sphingosine kinase 1 Homo sapiens 173-176 32006664-7 2020 Additionally, myristate regulated the expression of TNFalpha via JNK activation in an SK1-dependent manner, suggesting a novel S1PR-independent target as a mediator between SK1 and JNK in response to myristate. Myristates 200-209 sphingosine kinase 1 Homo sapiens 86-89 32006664-7 2020 Additionally, myristate regulated the expression of TNFalpha via JNK activation in an SK1-dependent manner, suggesting a novel S1PR-independent target as a mediator between SK1 and JNK in response to myristate. Myristates 200-209 sphingosine kinase 1 Homo sapiens 173-176 32006664-9 2020 Taken together our studies suggest that myristate regulates the expression of TNFalpha in the intestinal epithelium via regulation of SK1 and JNK. Myristates 40-49 sphingosine kinase 1 Homo sapiens 134-137 32637407-1 2020 Sphingosine-1-phosphate (S1P) is a pleiotropic sphingolipid derived by the phosphorylation of sphingosine either by sphingosine kinase 1 (SPHK1) or SPHK2. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 116-136 32637407-1 2020 Sphingosine-1-phosphate (S1P) is a pleiotropic sphingolipid derived by the phosphorylation of sphingosine either by sphingosine kinase 1 (SPHK1) or SPHK2. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 138-143 32637407-1 2020 Sphingosine-1-phosphate (S1P) is a pleiotropic sphingolipid derived by the phosphorylation of sphingosine either by sphingosine kinase 1 (SPHK1) or SPHK2. Sphingolipids 47-59 sphingosine kinase 1 Homo sapiens 116-136 32637407-1 2020 Sphingosine-1-phosphate (S1P) is a pleiotropic sphingolipid derived by the phosphorylation of sphingosine either by sphingosine kinase 1 (SPHK1) or SPHK2. Sphingolipids 47-59 sphingosine kinase 1 Homo sapiens 138-143 32637407-1 2020 Sphingosine-1-phosphate (S1P) is a pleiotropic sphingolipid derived by the phosphorylation of sphingosine either by sphingosine kinase 1 (SPHK1) or SPHK2. Sphingosine 94-105 sphingosine kinase 1 Homo sapiens 116-136 32637407-1 2020 Sphingosine-1-phosphate (S1P) is a pleiotropic sphingolipid derived by the phosphorylation of sphingosine either by sphingosine kinase 1 (SPHK1) or SPHK2. Sphingosine 94-105 sphingosine kinase 1 Homo sapiens 138-143 32526899-0 2020 Design and Development of Novel Urea, Sulfonyltriurea, and Sulfonamide Derivatives as Potential Inhibitors of Sphingosine Kinase 1. Urea 32-36 sphingosine kinase 1 Homo sapiens 110-130 32526899-0 2020 Design and Development of Novel Urea, Sulfonyltriurea, and Sulfonamide Derivatives as Potential Inhibitors of Sphingosine Kinase 1. sulfonyltriurea 38-53 sphingosine kinase 1 Homo sapiens 110-130 32526899-0 2020 Design and Development of Novel Urea, Sulfonyltriurea, and Sulfonamide Derivatives as Potential Inhibitors of Sphingosine Kinase 1. Sulfonamides 59-70 sphingosine kinase 1 Homo sapiens 110-130 32526899-3 2020 In search of effective SphK1 inhibitors, a set of small molecules have been designed and synthesized bearing urea, sulfonylurea, sulfonamide, and sulfonyltriurea groups. Urea 109-113 sphingosine kinase 1 Homo sapiens 23-28 32526899-3 2020 In search of effective SphK1 inhibitors, a set of small molecules have been designed and synthesized bearing urea, sulfonylurea, sulfonamide, and sulfonyltriurea groups. Sulfonylurea Compounds 115-127 sphingosine kinase 1 Homo sapiens 23-28 32526899-3 2020 In search of effective SphK1 inhibitors, a set of small molecules have been designed and synthesized bearing urea, sulfonylurea, sulfonamide, and sulfonyltriurea groups. Sulfonamides 129-140 sphingosine kinase 1 Homo sapiens 23-28 32526899-3 2020 In search of effective SphK1 inhibitors, a set of small molecules have been designed and synthesized bearing urea, sulfonylurea, sulfonamide, and sulfonyltriurea groups. sulfonyltriurea 146-161 sphingosine kinase 1 Homo sapiens 23-28 32526899-6 2020 Molecular docking studies revealed that these compounds fit well into the sphingosine binding pocket of SphK1 and formed significant number of hydrogen bonds and van der Waals interactions. Sphingosine 74-85 sphingosine kinase 1 Homo sapiens 104-109 32526899-6 2020 Molecular docking studies revealed that these compounds fit well into the sphingosine binding pocket of SphK1 and formed significant number of hydrogen bonds and van der Waals interactions. Hydrogen 143-151 sphingosine kinase 1 Homo sapiens 104-109 32596609-9 2020 In conclusion, we propose natural compounds, ZINC05434006 and ZINC04260971, as potential inhibitors of SphK1, which may be further exploited as potential leads to develop effective therapeutics against SphK1-associated diseases including cancer after in vitro and in vivo validations. zinc04260971 62-74 sphingosine kinase 1 Homo sapiens 103-108 32596609-9 2020 In conclusion, we propose natural compounds, ZINC05434006 and ZINC04260971, as potential inhibitors of SphK1, which may be further exploited as potential leads to develop effective therapeutics against SphK1-associated diseases including cancer after in vitro and in vivo validations. zinc04260971 62-74 sphingosine kinase 1 Homo sapiens 202-207 32521763-5 2020 Phosphorylation of sphingoid bases by sphingosine kinase 1 (SphK1) or SphK2 forms the penultimate step in the lysosomal catabolism of all sphingolipids. sphingoid bases 19-34 sphingosine kinase 1 Homo sapiens 38-58 32521763-5 2020 Phosphorylation of sphingoid bases by sphingosine kinase 1 (SphK1) or SphK2 forms the penultimate step in the lysosomal catabolism of all sphingolipids. sphingoid bases 19-34 sphingosine kinase 1 Homo sapiens 60-65 32521763-5 2020 Phosphorylation of sphingoid bases by sphingosine kinase 1 (SphK1) or SphK2 forms the penultimate step in the lysosomal catabolism of all sphingolipids. Sphingolipids 138-151 sphingosine kinase 1 Homo sapiens 38-58 32521763-5 2020 Phosphorylation of sphingoid bases by sphingosine kinase 1 (SphK1) or SphK2 forms the penultimate step in the lysosomal catabolism of all sphingolipids. Sphingolipids 138-151 sphingosine kinase 1 Homo sapiens 60-65 32521763-6 2020 Both SphK1 and SphK2 phosphorylated sphinga-4,14-diene as efficiently as sphingosine, however deuterated tracer experiments in an oligodendrocyte cell line demonstrated that ceramides with a sphingosine base are more rapidly metabolized than those with a sphingadiene base. sphinga-4,14-diene 36-54 sphingosine kinase 1 Homo sapiens 5-10 32596609-1 2020 Sphingosine kinase 1 (SphK1) is an oncogenic lipid kinase that catalyzes the formation of sphingosine-1-phosphate via phosphorylation of sphingosine and known to play a crucial role in angiogenesis, lymphocyte trafficking, signal transduction pathways, and response to apoptotic stimuli. sphingosine 1-phosphate 90-113 sphingosine kinase 1 Homo sapiens 22-27 32596609-1 2020 Sphingosine kinase 1 (SphK1) is an oncogenic lipid kinase that catalyzes the formation of sphingosine-1-phosphate via phosphorylation of sphingosine and known to play a crucial role in angiogenesis, lymphocyte trafficking, signal transduction pathways, and response to apoptotic stimuli. Sphingosine 90-101 sphingosine kinase 1 Homo sapiens 0-20 32596609-1 2020 Sphingosine kinase 1 (SphK1) is an oncogenic lipid kinase that catalyzes the formation of sphingosine-1-phosphate via phosphorylation of sphingosine and known to play a crucial role in angiogenesis, lymphocyte trafficking, signal transduction pathways, and response to apoptotic stimuli. Sphingosine 90-101 sphingosine kinase 1 Homo sapiens 22-27 32596609-7 2020 Two compounds, ZINC05434006 and ZINC04260971, bearing appreciable binding affinity and SphK1 selectivity were selected for 100 ns molecular dynamics (MD) simulations under explicit water conditions. zinc05434006 15-27 sphingosine kinase 1 Homo sapiens 87-92 32596609-7 2020 Two compounds, ZINC05434006 and ZINC04260971, bearing appreciable binding affinity and SphK1 selectivity were selected for 100 ns molecular dynamics (MD) simulations under explicit water conditions. zinc04260971 32-44 sphingosine kinase 1 Homo sapiens 87-92 32596609-8 2020 The all-atom MD simulation results suggested that the ZINC05434006 and ZINC04260971 binding induces a slight structural change and stabilizes the SphK1 structure. zinc05434006 54-66 sphingosine kinase 1 Homo sapiens 146-151 32596609-8 2020 The all-atom MD simulation results suggested that the ZINC05434006 and ZINC04260971 binding induces a slight structural change and stabilizes the SphK1 structure. zinc04260971 71-83 sphingosine kinase 1 Homo sapiens 146-151 32596609-9 2020 In conclusion, we propose natural compounds, ZINC05434006 and ZINC04260971, as potential inhibitors of SphK1, which may be further exploited as potential leads to develop effective therapeutics against SphK1-associated diseases including cancer after in vitro and in vivo validations. zinc05434006 45-57 sphingosine kinase 1 Homo sapiens 103-108 32596609-9 2020 In conclusion, we propose natural compounds, ZINC05434006 and ZINC04260971, as potential inhibitors of SphK1, which may be further exploited as potential leads to develop effective therapeutics against SphK1-associated diseases including cancer after in vitro and in vivo validations. zinc05434006 45-57 sphingosine kinase 1 Homo sapiens 202-207 32108663-10 2020 RESULTS: SphK1 mRNA expression was higher in cells from septic patients versus healthy volunteers (septic patients vs. healthy volunteers: 50.9 +- 57.0 fold change vs. 1.2 +- 0.1 fold change, P < 0.0001) and was positively correlated with IL-1beta mRNA expression in these cells (r = 0.537, P = 0.012) and negatively correlated with PaO2/FIO2 ratios (r = 0.516, P = 0.017). fio2 338-342 sphingosine kinase 1 Homo sapiens 9-14 32251679-9 2020 An acute (10 min) exposure of OPCs to BAY60-6583 also increased the phosphorylated form of sphingosine kinase 1 (SphK1). BAY 60-6583 38-48 sphingosine kinase 1 Homo sapiens 91-111 32251679-9 2020 An acute (10 min) exposure of OPCs to BAY60-6583 also increased the phosphorylated form of sphingosine kinase 1 (SphK1). BAY 60-6583 38-48 sphingosine kinase 1 Homo sapiens 113-118 32251679-11 2020 Furthermore, A2BR was overexpressed during OPC differentiation, an effect prevented by the pan SphK1/2 inhibitor VPC69047. a2br 13-17 sphingosine kinase 1 Homo sapiens 95-102 32251679-11 2020 Furthermore, A2BR was overexpressed during OPC differentiation, an effect prevented by the pan SphK1/2 inhibitor VPC69047. vpc69047 113-121 sphingosine kinase 1 Homo sapiens 95-102 31827236-2 2020 Sphingosine-1-phosphate (S1P), whose formation is catalyzed by sphingosine kinase (SPHK)-1 or -2, is a bioactive lipid implicated in human health and disease. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 63-96 31827236-2 2020 Sphingosine-1-phosphate (S1P), whose formation is catalyzed by sphingosine kinase (SPHK)-1 or -2, is a bioactive lipid implicated in human health and disease. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 63-96 32626514-5 2020 In the current study, the anti-DIPG activity of ABC294640, the only first-in-class orally available Sphingosine kinase (SphK) inhibitor was explored. 3-(4-chlorophenyl)-adamantane-1-carboxylic acid (pyridin-4-ylmethyl)amide 48-57 sphingosine kinase 1 Homo sapiens 100-118 32278039-1 2020 Sphingosine-1-phosphate (S1P) is a signaling lipid, synthetized by sphingosine kinases (SPHK1 and SPHK2), that affects cardiovascular function in various ways. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 88-93 32278039-1 2020 Sphingosine-1-phosphate (S1P) is a signaling lipid, synthetized by sphingosine kinases (SPHK1 and SPHK2), that affects cardiovascular function in various ways. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 88-93 32471162-5 2020 PF-543 derivatives in which the methyl group was substituted by hydrogen and fluorine (compound 5) demonstrated SK1 inhibitory and anticancer activities similar to PF-543. Fluorine 77-85 sphingosine kinase 1 Homo sapiens 112-115 32626514-5 2020 In the current study, the anti-DIPG activity of ABC294640, the only first-in-class orally available Sphingosine kinase (SphK) inhibitor was explored. 3-(4-chlorophenyl)-adamantane-1-carboxylic acid (pyridin-4-ylmethyl)amide 48-57 sphingosine kinase 1 Homo sapiens 120-124 32260399-6 2020 We further demonstrated that SPHK1 inhibitors, FTY720 and PF543, synergized with doxorubicin in targeting both breast CSCs and non-CSCs. Doxorubicin 81-92 sphingosine kinase 1 Homo sapiens 29-34 31814336-2 2020 Nitric oxide (NO) is also known to play a role in vasoactive properties, whether Sphk1/S1P signaling is able to alter angiogenesis in the context of cerebral ischemia-reperfusion injury (IRI), and whether such activity is linked with NO production, however, remains uncertain. sphingosine 1-phosphate 87-90 sphingosine kinase 1 Homo sapiens 81-86 31814336-3 2020 METHODS: We used immunofluorescence to detect the expression of Sphk1 and NOS in cerebral epithelial cells (EC) after IR or oxygen-glucose deprivation (OGDR). Oxygen 124-130 sphingosine kinase 1 Homo sapiens 64-69 31814336-3 2020 METHODS: We used immunofluorescence to detect the expression of Sphk1 and NOS in cerebral epithelial cells (EC) after IR or oxygen-glucose deprivation (OGDR). Glucose 131-138 sphingosine kinase 1 Homo sapiens 64-69 31814336-9 2020 In HBMECs, knocking down Sphk1 markedly reduced NO production owing to reduced eNOS activity, and inhibiting eNOS directly similarly decreased NO production in a manner which could be reversed via exogenously treating cells with S1P. sphingosine 1-phosphate 229-232 sphingosine kinase 1 Homo sapiens 25-30 31814336-11 2020 CONCLUSIONS: Based on these results, we therefore postulate that Sphk1/S1P signaling is able to mediate angiogenesis following cerebral IRI via the regulation of eNOS activity and NO production. sphingosine 1-phosphate 71-74 sphingosine kinase 1 Homo sapiens 65-70 32371868-7 2020 I-BET726 not only downregulated BRD4-regulated proteins (c-Myc, Bcl-2, and cyclin D1), but also inhibited sphingosine kinase 1 (SphK1) and Akt signalings in SCC cells. 4-(1-acetyl-4-((4-chlorophenyl)amino)-2-methyl-1,2,3,4-tetrahydroquinolin-6-yl)benzoic acid 0-8 sphingosine kinase 1 Homo sapiens 106-126 32371868-7 2020 I-BET726 not only downregulated BRD4-regulated proteins (c-Myc, Bcl-2, and cyclin D1), but also inhibited sphingosine kinase 1 (SphK1) and Akt signalings in SCC cells. 4-(1-acetyl-4-((4-chlorophenyl)amino)-2-methyl-1,2,3,4-tetrahydroquinolin-6-yl)benzoic acid 0-8 sphingosine kinase 1 Homo sapiens 128-133 32371868-10 2020 Downregulation of BRD4-regulated proteins and inhibition of the SphK1-Akt signaling were detected in I-BET726-treated A431 xenograft tumor tissues. 4-(1-acetyl-4-((4-chlorophenyl)amino)-2-methyl-1,2,3,4-tetrahydroquinolin-6-yl)benzoic acid 101-109 sphingosine kinase 1 Homo sapiens 64-69 31703810-1 2020 2-Epi-jaspine B is an isomer of the natural product jaspine B and shows certain selectivity for SphK1 and potent antitumor activity. pachastrissamine 0-15 sphingosine kinase 1 Homo sapiens 96-101 31703810-1 2020 2-Epi-jaspine B is an isomer of the natural product jaspine B and shows certain selectivity for SphK1 and potent antitumor activity. pachastrissamine 6-15 sphingosine kinase 1 Homo sapiens 96-101 31703810-2 2020 Based on the crystal structure of SphK1, we transformed the structure of 2-epi-jaspine B and modified the hydrophobic side chain to obtain a series of 2-epi-jaspine B analogs. pachastrissamine 73-88 sphingosine kinase 1 Homo sapiens 34-39 31703810-2 2020 Based on the crystal structure of SphK1, we transformed the structure of 2-epi-jaspine B and modified the hydrophobic side chain to obtain a series of 2-epi-jaspine B analogs. pachastrissamine 151-166 sphingosine kinase 1 Homo sapiens 34-39 31703810-5 2020 Furthermore, YHR17 inhibited SphK1 with more than 125-fold selectivity over SphK2. yhr17 13-18 sphingosine kinase 1 Homo sapiens 29-34 32266132-3 2020 SK1 is upregulated by estrogen signaling, which, in turn, confers cancer cells with resistance to tamoxifen. Tamoxifen 98-107 sphingosine kinase 1 Homo sapiens 0-3 32435405-4 2020 In this framework, we designed a series of natural monosaccharide-based compounds to enhance anchoring of the known SK1 inhibitor PF-543 at the polar head of the J-shaped substrate-binding channel. Monosaccharides 51-65 sphingosine kinase 1 Homo sapiens 116-119 32435405-4 2020 In this framework, we designed a series of natural monosaccharide-based compounds to enhance anchoring of the known SK1 inhibitor PF-543 at the polar head of the J-shaped substrate-binding channel. PF-543 130-136 sphingosine kinase 1 Homo sapiens 116-119 32266132-4 2020 Sphingosine-1-phosphate (S1P) produced by SK1 has been linked to tumor invasion and metastasis. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 42-45 32266132-4 2020 Sphingosine-1-phosphate (S1P) produced by SK1 has been linked to tumor invasion and metastasis. sphingosine 1-phosphate 25-28 sphingosine kinase 1 Homo sapiens 42-45 32266132-10 2020 This review summarizes the up-to-date evidence and discusses the therapeutic potential for the SK1 inhibition in breast cancer, with emphasis on the mechanisms of chemoresistance and combination with other therapies such as gefitinib or docetaxel. Docetaxel 237-246 sphingosine kinase 1 Homo sapiens 95-98 31917989-0 2020 Investigation of guanidinium chloride-induced unfolding pathway of sphingosine kinase 1. Guanidine 17-37 sphingosine kinase 1 Homo sapiens 67-87 32203055-8 2020 MiR-3677 overexpression-induced anti-OS cell activity was reversed with re-expression of the 3"-UTR-depleted SphK1. mir-3677 0-8 sphingosine kinase 1 Homo sapiens 109-114 31917989-2 2020 In the present study, we investigated the guanidinium chloride (GdmCl)-induced denaturation of SphK1 at pH 8.0 and 25 C using two different spectroscopic probes, i.e., mean residue ellipticity at 222 nm ([theta]222) and fluorescence emission maxima (lambdamax). Guanidine 42-62 sphingosine kinase 1 Homo sapiens 95-100 31917989-2 2020 In the present study, we investigated the guanidinium chloride (GdmCl)-induced denaturation of SphK1 at pH 8.0 and 25 C using two different spectroscopic probes, i.e., mean residue ellipticity at 222 nm ([theta]222) and fluorescence emission maxima (lambdamax). Guanidine 64-69 sphingosine kinase 1 Homo sapiens 95-100 31917989-3 2020 A significant overlap between the transition curves obtained from both the spectral properties indicate that GdmCl-induced unfolding of SphK1 follows two-state process i.e., Native (N) Denatured (D) state. Guanidine 109-114 sphingosine kinase 1 Homo sapiens 136-141 29460151-1 2020 There is substantial evidence that the enzymes, sphingosine kinase 1 and 2, which catalyse the formation of the bioactive lipid sphingosine 1-phosphate, are involved in pathophysiological processes. sphingosine 1-phosphate 128-151 sphingosine kinase 1 Homo sapiens 48-74 31971297-2 2020 Here, we report that sphingosine kinase 1 (SphK1) which produces the bioactive sphingolipid metabolite, sphingosine 1-phosphate (S1P), plays a critical role in the pDC functions and interferon production. Sphingolipids 79-91 sphingosine kinase 1 Homo sapiens 21-41 31971297-2 2020 Here, we report that sphingosine kinase 1 (SphK1) which produces the bioactive sphingolipid metabolite, sphingosine 1-phosphate (S1P), plays a critical role in the pDC functions and interferon production. Sphingolipids 79-91 sphingosine kinase 1 Homo sapiens 43-48 31971297-2 2020 Here, we report that sphingosine kinase 1 (SphK1) which produces the bioactive sphingolipid metabolite, sphingosine 1-phosphate (S1P), plays a critical role in the pDC functions and interferon production. sphingosine 1-phosphate 104-127 sphingosine kinase 1 Homo sapiens 21-41 31971297-2 2020 Here, we report that sphingosine kinase 1 (SphK1) which produces the bioactive sphingolipid metabolite, sphingosine 1-phosphate (S1P), plays a critical role in the pDC functions and interferon production. sphingosine 1-phosphate 104-127 sphingosine kinase 1 Homo sapiens 43-48 31971297-2 2020 Here, we report that sphingosine kinase 1 (SphK1) which produces the bioactive sphingolipid metabolite, sphingosine 1-phosphate (S1P), plays a critical role in the pDC functions and interferon production. sphingosine 1-phosphate 129-132 sphingosine kinase 1 Homo sapiens 21-41 31971297-2 2020 Here, we report that sphingosine kinase 1 (SphK1) which produces the bioactive sphingolipid metabolite, sphingosine 1-phosphate (S1P), plays a critical role in the pDC functions and interferon production. sphingosine 1-phosphate 129-132 sphingosine kinase 1 Homo sapiens 43-48 31971297-7 2020 Taken together, our results indicate a pivotal regulatory role for the SphK1/S1P axis in maintaining the balance between immunosurveillance and immunopathology and suggest that specific SphK1 inhibitors might be a new therapeutic avenue for the treatment of type I IFN-linked autoimmune disorders. sphingosine 1-phosphate 77-80 sphingosine kinase 1 Homo sapiens 71-76 31971297-7 2020 Taken together, our results indicate a pivotal regulatory role for the SphK1/S1P axis in maintaining the balance between immunosurveillance and immunopathology and suggest that specific SphK1 inhibitors might be a new therapeutic avenue for the treatment of type I IFN-linked autoimmune disorders. sphingosine 1-phosphate 77-80 sphingosine kinase 1 Homo sapiens 186-191 31863815-2 2020 GI tumor and inflammatory cells contain activated sphingolipid-metabolizing enzymes, including sphingosine kinase 1 (SphK1) and SphK2, that generate sphingosine-1-phosphate (S1P), a highly bioactive compound. Sphingolipids 50-62 sphingosine kinase 1 Homo sapiens 95-115 31863815-2 2020 GI tumor and inflammatory cells contain activated sphingolipid-metabolizing enzymes, including sphingosine kinase 1 (SphK1) and SphK2, that generate sphingosine-1-phosphate (S1P), a highly bioactive compound. Sphingolipids 50-62 sphingosine kinase 1 Homo sapiens 117-122 31863815-2 2020 GI tumor and inflammatory cells contain activated sphingolipid-metabolizing enzymes, including sphingosine kinase 1 (SphK1) and SphK2, that generate sphingosine-1-phosphate (S1P), a highly bioactive compound. sphingosine 1-phosphate 149-172 sphingosine kinase 1 Homo sapiens 95-115 31863815-2 2020 GI tumor and inflammatory cells contain activated sphingolipid-metabolizing enzymes, including sphingosine kinase 1 (SphK1) and SphK2, that generate sphingosine-1-phosphate (S1P), a highly bioactive compound. sphingosine 1-phosphate 149-172 sphingosine kinase 1 Homo sapiens 117-122 31863815-2 2020 GI tumor and inflammatory cells contain activated sphingolipid-metabolizing enzymes, including sphingosine kinase 1 (SphK1) and SphK2, that generate sphingosine-1-phosphate (S1P), a highly bioactive compound. sphingosine 1-phosphate 174-177 sphingosine kinase 1 Homo sapiens 95-115 31863815-2 2020 GI tumor and inflammatory cells contain activated sphingolipid-metabolizing enzymes, including sphingosine kinase 1 (SphK1) and SphK2, that generate sphingosine-1-phosphate (S1P), a highly bioactive compound. sphingosine 1-phosphate 174-177 sphingosine kinase 1 Homo sapiens 117-122 31863815-4 2020 High fat and sugar diet, disbalanced intestinal flora, and obesity have recently been linked to activation of inflammation and SphK/S1P/S1P receptor (S1PR) signaling in various GI pathologies, including cancer. Carbohydrates 13-18 sphingosine kinase 1 Homo sapiens 127-131 31863815-4 2020 High fat and sugar diet, disbalanced intestinal flora, and obesity have recently been linked to activation of inflammation and SphK/S1P/S1P receptor (S1PR) signaling in various GI pathologies, including cancer. sphingosine 1-phosphate 132-135 sphingosine kinase 1 Homo sapiens 127-131 31863815-4 2020 High fat and sugar diet, disbalanced intestinal flora, and obesity have recently been linked to activation of inflammation and SphK/S1P/S1P receptor (S1PR) signaling in various GI pathologies, including cancer. sphingosine 1-phosphate 136-139 sphingosine kinase 1 Homo sapiens 127-131 31863815-6 2020 SphK/S1P axis, a mediator of inflammation in the tumor microenvironment, has recently been defined as a target for the treatment of GI disease states, including inflammatory bowel disease and colitis. sphingosine 1-phosphate 5-8 sphingosine kinase 1 Homo sapiens 0-4 32085881-6 2020 All GI tissues and circulating immune/blood cells contain activated sphingolipid-metabolizing enzymes, including sphingosine kinases (SphK1 and SphK2) that generate sphingosine-1-phosphate (S1P), a bioactive lipid and ligand for five G-protein coupled membrane S1P receptors (S1PRs). Sphingolipids 68-80 sphingosine kinase 1 Homo sapiens 134-139 32085881-6 2020 All GI tissues and circulating immune/blood cells contain activated sphingolipid-metabolizing enzymes, including sphingosine kinases (SphK1 and SphK2) that generate sphingosine-1-phosphate (S1P), a bioactive lipid and ligand for five G-protein coupled membrane S1P receptors (S1PRs). sphingosine 1-phosphate 165-188 sphingosine kinase 1 Homo sapiens 134-139 31648078-3 2020 Sphingosine kinases 1 and 2 (SK1 and SK2) catalyze the conversion of the sphingolipid metabolite sphingosine into S1P. Sphingolipids 73-85 sphingosine kinase 1 Homo sapiens 0-27 31648078-3 2020 Sphingosine kinases 1 and 2 (SK1 and SK2) catalyze the conversion of the sphingolipid metabolite sphingosine into S1P. Sphingolipids 73-85 sphingosine kinase 1 Homo sapiens 29-32 31648078-3 2020 Sphingosine kinases 1 and 2 (SK1 and SK2) catalyze the conversion of the sphingolipid metabolite sphingosine into S1P. Sphingosine 97-108 sphingosine kinase 1 Homo sapiens 0-27 31648078-3 2020 Sphingosine kinases 1 and 2 (SK1 and SK2) catalyze the conversion of the sphingolipid metabolite sphingosine into S1P. Sphingosine 97-108 sphingosine kinase 1 Homo sapiens 29-32 31544710-5 2020 RESULTS: HCC is characterised by dysregulation of ceramide metabolism, which could be ascribed to altered activity and expression of ceramide synthases 2, 4 and 6, and acid and alkaline ceramidases 2 and 3, as well as to deregulation of sphingosine kinases (SphK) 1 and 2 and sphingosine-1-phosphate receptors, in particular S1PR1. Ceramides 50-58 sphingosine kinase 1 Homo sapiens 237-271 31895563-2 2020 The formation of S1P is catalyzed by sphingosine kinase 1 or 2 (SphK1 or SphK2) from sphingosine (Sph) and ATP. Sphingosine 37-48 sphingosine kinase 1 Homo sapiens 64-69 31895563-2 2020 The formation of S1P is catalyzed by sphingosine kinase 1 or 2 (SphK1 or SphK2) from sphingosine (Sph) and ATP. Sphingosine 64-67 sphingosine kinase 1 Homo sapiens 37-62 31895563-2 2020 The formation of S1P is catalyzed by sphingosine kinase 1 or 2 (SphK1 or SphK2) from sphingosine (Sph) and ATP. Adenosine Triphosphate 107-110 sphingosine kinase 1 Homo sapiens 37-62 31895563-2 2020 The formation of S1P is catalyzed by sphingosine kinase 1 or 2 (SphK1 or SphK2) from sphingosine (Sph) and ATP. Adenosine Triphosphate 107-110 sphingosine kinase 1 Homo sapiens 64-69 31446356-1 2020 Sphingosine kinase 1 (SphK1) catalyzes the conversion of sphingosine to sphingosine-1-phosphate that acts as a bioactive signalling molecule, and regulates various cellular processes including lymphocyte trafficking, angiogenesis and response to apoptotic stimuli. Sphingosine 57-68 sphingosine kinase 1 Homo sapiens 0-20 31446356-1 2020 Sphingosine kinase 1 (SphK1) catalyzes the conversion of sphingosine to sphingosine-1-phosphate that acts as a bioactive signalling molecule, and regulates various cellular processes including lymphocyte trafficking, angiogenesis and response to apoptotic stimuli. Sphingosine 57-68 sphingosine kinase 1 Homo sapiens 22-27 31446356-1 2020 Sphingosine kinase 1 (SphK1) catalyzes the conversion of sphingosine to sphingosine-1-phosphate that acts as a bioactive signalling molecule, and regulates various cellular processes including lymphocyte trafficking, angiogenesis and response to apoptotic stimuli. sphingosine 1-phosphate 72-95 sphingosine kinase 1 Homo sapiens 0-20 31446356-1 2020 Sphingosine kinase 1 (SphK1) catalyzes the conversion of sphingosine to sphingosine-1-phosphate that acts as a bioactive signalling molecule, and regulates various cellular processes including lymphocyte trafficking, angiogenesis and response to apoptotic stimuli. sphingosine 1-phosphate 72-95 sphingosine kinase 1 Homo sapiens 22-27 31757412-4 2020 In a search for new SphK1 inhibitors we selected the well-known SKI-II inhibitor as the starting structure and we synthesized a new inhibitor structurally related to SKI-II with a significant but moderate inhibitory effect. N'-((2-hydroxy-1-naphthyl)methylene)-3-(2-naphthyl)-1H-pyrazole-5-carbohydrazide 64-70 sphingosine kinase 1 Homo sapiens 20-25 31757412-4 2020 In a search for new SphK1 inhibitors we selected the well-known SKI-II inhibitor as the starting structure and we synthesized a new inhibitor structurally related to SKI-II with a significant but moderate inhibitory effect. N'-((2-hydroxy-1-naphthyl)methylene)-3-(2-naphthyl)-1H-pyrazole-5-carbohydrazide 166-172 sphingosine kinase 1 Homo sapiens 20-25 33317322-1 2020 Sphingosine kinase 1 (SPHK1) regulates cell proliferation and survival by converting sphingosine to the signaling mediator sphingosine 1-phosphate (S1P). Sphingosine 85-96 sphingosine kinase 1 Homo sapiens 0-20 33317322-1 2020 Sphingosine kinase 1 (SPHK1) regulates cell proliferation and survival by converting sphingosine to the signaling mediator sphingosine 1-phosphate (S1P). Sphingosine 85-96 sphingosine kinase 1 Homo sapiens 22-27 33317322-1 2020 Sphingosine kinase 1 (SPHK1) regulates cell proliferation and survival by converting sphingosine to the signaling mediator sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 123-146 sphingosine kinase 1 Homo sapiens 0-20 33317322-1 2020 Sphingosine kinase 1 (SPHK1) regulates cell proliferation and survival by converting sphingosine to the signaling mediator sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 123-146 sphingosine kinase 1 Homo sapiens 22-27 33317322-1 2020 Sphingosine kinase 1 (SPHK1) regulates cell proliferation and survival by converting sphingosine to the signaling mediator sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 148-151 sphingosine kinase 1 Homo sapiens 0-20 33317322-1 2020 Sphingosine kinase 1 (SPHK1) regulates cell proliferation and survival by converting sphingosine to the signaling mediator sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 148-151 sphingosine kinase 1 Homo sapiens 22-27 31321542-3 2020 Sphingosine-1-phosphate (S1P) is a pleiotropic lipid signaling molecule produced by two isoforms of sphingosine kinase (SphK1 and SphK2) that is involved in regulation of many aspects of mammalian physiology and pathophysiology, including inflammation, epithelial and endothelial barrier function, cancer, and metastasis, among many others. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 120-125 31809267-3 2019 The phosphorylation of sphingosine by sphingosine kinase enzymes SphK1 and SphK2 generates sphingosine-1-phosphate (S1P), which inhibits human chondrosarcoma cell migration, while SphK1 overexpression suppresses lung metastasis of chondrosarcoma. Sphingosine 23-34 sphingosine kinase 1 Homo sapiens 65-70 31809267-3 2019 The phosphorylation of sphingosine by sphingosine kinase enzymes SphK1 and SphK2 generates sphingosine-1-phosphate (S1P), which inhibits human chondrosarcoma cell migration, while SphK1 overexpression suppresses lung metastasis of chondrosarcoma. Sphingosine 23-34 sphingosine kinase 1 Homo sapiens 180-185 31809267-3 2019 The phosphorylation of sphingosine by sphingosine kinase enzymes SphK1 and SphK2 generates sphingosine-1-phosphate (S1P), which inhibits human chondrosarcoma cell migration, while SphK1 overexpression suppresses lung metastasis of chondrosarcoma. Sphingosine 38-49 sphingosine kinase 1 Homo sapiens 65-70 31809267-3 2019 The phosphorylation of sphingosine by sphingosine kinase enzymes SphK1 and SphK2 generates sphingosine-1-phosphate (S1P), which inhibits human chondrosarcoma cell migration, while SphK1 overexpression suppresses lung metastasis of chondrosarcoma. Sphingosine 38-49 sphingosine kinase 1 Homo sapiens 180-185 31809267-3 2019 The phosphorylation of sphingosine by sphingosine kinase enzymes SphK1 and SphK2 generates sphingosine-1-phosphate (S1P), which inhibits human chondrosarcoma cell migration, while SphK1 overexpression suppresses lung metastasis of chondrosarcoma. sphingosine 1-phosphate 91-114 sphingosine kinase 1 Homo sapiens 65-70 31809267-3 2019 The phosphorylation of sphingosine by sphingosine kinase enzymes SphK1 and SphK2 generates sphingosine-1-phosphate (S1P), which inhibits human chondrosarcoma cell migration, while SphK1 overexpression suppresses lung metastasis of chondrosarcoma. sphingosine 1-phosphate 116-119 sphingosine kinase 1 Homo sapiens 65-70 31809267-3 2019 The phosphorylation of sphingosine by sphingosine kinase enzymes SphK1 and SphK2 generates sphingosine-1-phosphate (S1P), which inhibits human chondrosarcoma cell migration, while SphK1 overexpression suppresses lung metastasis of chondrosarcoma. sphingosine 1-phosphate 116-119 sphingosine kinase 1 Homo sapiens 180-185 31197942-0 2019 Furowanin A Exhibits Antiproliferative and Pro-Apoptotic Activities by Targeting Sphingosine Kinase 1 in Osteosarcoma. Furowanin A 0-11 sphingosine kinase 1 Homo sapiens 81-101 31822735-2 2019 Here, we have investigated the binding affinity of dietary phytochemicals viz., ursolic acid, capsaicin, DL-alpha tocopherol acetate, quercetin, vanillin, citral, limonin and simvastatin with the SphK1. alpha-Tocopherol 105-132 sphingosine kinase 1 Homo sapiens 196-201 31822735-2 2019 Here, we have investigated the binding affinity of dietary phytochemicals viz., ursolic acid, capsaicin, DL-alpha tocopherol acetate, quercetin, vanillin, citral, limonin and simvastatin with the SphK1. Simvastatin 175-186 sphingosine kinase 1 Homo sapiens 196-201 31822735-4 2019 Fluorescence binding and isothermal titration calorimetric measurements suggested that quercetin and capsaicin bind to SphK1 with an excellent affinity, and significantly inhibits its activity with an admirable IC50 values. Quercetin 87-96 sphingosine kinase 1 Homo sapiens 119-124 31822735-4 2019 Fluorescence binding and isothermal titration calorimetric measurements suggested that quercetin and capsaicin bind to SphK1 with an excellent affinity, and significantly inhibits its activity with an admirable IC50 values. Capsaicin 101-110 sphingosine kinase 1 Homo sapiens 119-124 31822735-7 2019 Quercetin forms a stable complex with SphK1 without inducing any significant conformational changes in the protein structure. Quercetin 0-9 sphingosine kinase 1 Homo sapiens 38-43 31822735-8 2019 In conclusion, we infer that quercetin and capsaicin provide a chemical scaffold to develop potent and selective inhibitors of SphK1 after required modifications for the clinical management of cancer. Quercetin 29-38 sphingosine kinase 1 Homo sapiens 127-132 31822735-8 2019 In conclusion, we infer that quercetin and capsaicin provide a chemical scaffold to develop potent and selective inhibitors of SphK1 after required modifications for the clinical management of cancer. Capsaicin 43-52 sphingosine kinase 1 Homo sapiens 127-132 31810327-2 2019 Sphingosine kinase (SK), which transforms sphingosine into S1P, has two isotypes: SK1 and SK2. Sphingosine 0-11 sphingosine kinase 1 Homo sapiens 82-85 31810327-2 2019 Sphingosine kinase (SK), which transforms sphingosine into S1P, has two isotypes: SK1 and SK2. Sphingosine 42-53 sphingosine kinase 1 Homo sapiens 82-85 31810327-2 2019 Sphingosine kinase (SK), which transforms sphingosine into S1P, has two isotypes: SK1 and SK2. sphingosine 1-phosphate 59-62 sphingosine kinase 1 Homo sapiens 82-85 31810327-8 2019 Boron-dipyrromethene (BODIPY)-introduced PF-543 has a similar SK1 inhibitory effect as PF-543. 3'-boron-dipyrromethene-flavin 0-20 sphingosine kinase 1 Homo sapiens 62-65 31810327-8 2019 Boron-dipyrromethene (BODIPY)-introduced PF-543 has a similar SK1 inhibitory effect as PF-543. 3'-boron-dipyrromethene-flavin 22-28 sphingosine kinase 1 Homo sapiens 62-65 31817453-8 2019 This suggests that the anti-cancer effect of ISO can be regulated by SPHK/tubulin destabilization pathways. isorhapontigenin 45-48 sphingosine kinase 1 Homo sapiens 69-73 34057489-3 2019 From the spectrophotometric experiments, it was seen that compound SK-1 displayed an excellent fluorescence response towards targeted aluminum ions probably due to its suitable chelating structure. Aluminum 134-142 sphingosine kinase 1 Homo sapiens 67-71 34057489-4 2019 Furthermore, such compound SK-1 also showed high sensitivity and selectivity response towards aluminum ions over other competing ions. Aluminum 94-102 sphingosine kinase 1 Homo sapiens 27-31 34057489-5 2019 In addition, the potential biological applications of SK-1 to detect aluminum ions in living cells were also investigated and results showed that fluorescence sensor SK-1 could be a promising probe for determining and/or monitoring aluminum ions in both biological and/or chemical samples. Aluminum 69-77 sphingosine kinase 1 Homo sapiens 166-170 34057489-5 2019 In addition, the potential biological applications of SK-1 to detect aluminum ions in living cells were also investigated and results showed that fluorescence sensor SK-1 could be a promising probe for determining and/or monitoring aluminum ions in both biological and/or chemical samples. Aluminum 232-240 sphingosine kinase 1 Homo sapiens 166-170 31097785-2 2019 We show that sphingosine kinase-1 (SPHK1), which generates the potent bioactive sphingolipid sphingosine-1-phosphate (S1P), is over-expressed in DLBCL. sphingolipid sphingosine-1-phosphate 80-116 sphingosine kinase 1 Homo sapiens 13-33 31097785-2 2019 We show that sphingosine kinase-1 (SPHK1), which generates the potent bioactive sphingolipid sphingosine-1-phosphate (S1P), is over-expressed in DLBCL. sphingolipid sphingosine-1-phosphate 80-116 sphingosine kinase 1 Homo sapiens 35-40 31652307-0 2019 Kelch-like protein 5-mediated ubiquitination of lysine 183 promotes proteasomal degradation of sphingosine kinase 1. tyrosyl-lysine 48-54 sphingosine kinase 1 Homo sapiens 95-115 31652307-1 2019 Sphingosine kinase 1 (SK1) is a signaling enzyme that catalyzes the phosphorylation of sphingosine to generate the bioactive lipid sphingosine 1-phosphate (S1P). Sphingosine 87-98 sphingosine kinase 1 Homo sapiens 0-20 31652307-1 2019 Sphingosine kinase 1 (SK1) is a signaling enzyme that catalyzes the phosphorylation of sphingosine to generate the bioactive lipid sphingosine 1-phosphate (S1P). Sphingosine 87-98 sphingosine kinase 1 Homo sapiens 22-25 31652307-1 2019 Sphingosine kinase 1 (SK1) is a signaling enzyme that catalyzes the phosphorylation of sphingosine to generate the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 131-154 sphingosine kinase 1 Homo sapiens 0-20 31652307-1 2019 Sphingosine kinase 1 (SK1) is a signaling enzyme that catalyzes the phosphorylation of sphingosine to generate the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 131-154 sphingosine kinase 1 Homo sapiens 22-25 31652307-1 2019 Sphingosine kinase 1 (SK1) is a signaling enzyme that catalyzes the phosphorylation of sphingosine to generate the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 156-159 sphingosine kinase 1 Homo sapiens 0-20 31652307-1 2019 Sphingosine kinase 1 (SK1) is a signaling enzyme that catalyzes the phosphorylation of sphingosine to generate the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 156-159 sphingosine kinase 1 Homo sapiens 22-25 31807117-2 2019 It is formed by one of two sphingosine kinases (SphKs), SphK1 and SphK2. Sphingosine 27-38 sphingosine kinase 1 Homo sapiens 56-61 31197942-6 2019 To validate the role of SphK1 in the pro-apoptotic activity of Fur A, overexpressing SphK1 vector and siRNA targeting SphK1 were utilized to transfect OS cells. fur a 63-68 sphingosine kinase 1 Homo sapiens 24-29 31197942-10 2019 Our results showed that the anticancer activity of Fur A was associated with downregulation of SphK1 and inactivation of its downstream signaling. fur a 51-56 sphingosine kinase 1 Homo sapiens 95-100 31197942-11 2019 The mediatory role of SphK1 was validated when the pro-apoptotic activity of Fur A was significantly blocked by SphK1 overexpression, while SphK1 knockdown sensitized the OS cells to Fur A. fur a 77-82 sphingosine kinase 1 Homo sapiens 22-27 31197942-11 2019 The mediatory role of SphK1 was validated when the pro-apoptotic activity of Fur A was significantly blocked by SphK1 overexpression, while SphK1 knockdown sensitized the OS cells to Fur A. fur a 77-82 sphingosine kinase 1 Homo sapiens 112-117 31197942-11 2019 The mediatory role of SphK1 was validated when the pro-apoptotic activity of Fur A was significantly blocked by SphK1 overexpression, while SphK1 knockdown sensitized the OS cells to Fur A. fur a 77-82 sphingosine kinase 1 Homo sapiens 112-117 31197942-11 2019 The mediatory role of SphK1 was validated when the pro-apoptotic activity of Fur A was significantly blocked by SphK1 overexpression, while SphK1 knockdown sensitized the OS cells to Fur A. fur a 183-188 sphingosine kinase 1 Homo sapiens 22-27 31197942-12 2019 We concluded that Fur A can exhibit anti-growth and pro-apoptotic activities in vitro and in vivo in OS by downregulating SphK1. fur a 18-23 sphingosine kinase 1 Homo sapiens 122-127 31352240-8 2019 EA binds to the SphK1 and forms several interactions with catalytically important residues of ATP-binding pocket. Ellagic Acid 0-2 sphingosine kinase 1 Homo sapiens 16-21 31620198-1 2019 Sphingosine kinase 1 (SPHK1), an ATP-dependent protein, has previously been demonstrated to be upregulated in several types of human cancer and to play an important role in tumor development and progression. Adenosine Triphosphate 33-36 sphingosine kinase 1 Homo sapiens 0-20 31620198-1 2019 Sphingosine kinase 1 (SPHK1), an ATP-dependent protein, has previously been demonstrated to be upregulated in several types of human cancer and to play an important role in tumor development and progression. Adenosine Triphosphate 33-36 sphingosine kinase 1 Homo sapiens 22-27 31352240-0 2019 Evaluation of ellagic acid as an inhibitor of sphingosine kinase 1: A targeted approach towards anticancer therapy. Ellagic Acid 14-26 sphingosine kinase 1 Homo sapiens 46-66 31352240-1 2019 Sphingosine kinase 1 (SphK1) is one of the central enzymes of sphingolipid metabolism whose high expression level is presumed to be correlated with cancer and other inflammatory diseases. Sphingolipids 62-74 sphingosine kinase 1 Homo sapiens 0-20 31352240-1 2019 Sphingosine kinase 1 (SphK1) is one of the central enzymes of sphingolipid metabolism whose high expression level is presumed to be correlated with cancer and other inflammatory diseases. Sphingolipids 62-74 sphingosine kinase 1 Homo sapiens 22-27 31352240-2 2019 Using a virtual screening approach and in vitro studies, we have identified the ellagic acid (EA), a dietary polyphenol, as a potent inhibitor of SphK1. Ellagic Acid 80-92 sphingosine kinase 1 Homo sapiens 146-151 31352240-2 2019 Using a virtual screening approach and in vitro studies, we have identified the ellagic acid (EA), a dietary polyphenol, as a potent inhibitor of SphK1. Ellagic Acid 94-96 sphingosine kinase 1 Homo sapiens 146-151 31352240-2 2019 Using a virtual screening approach and in vitro studies, we have identified the ellagic acid (EA), a dietary polyphenol, as a potent inhibitor of SphK1. Polyphenols 109-119 sphingosine kinase 1 Homo sapiens 146-151 31352240-3 2019 Molecular docking study has suggested a strong binding affinity of EA to the SphK1. Ellagic Acid 67-69 sphingosine kinase 1 Homo sapiens 77-82 31352240-8 2019 EA binds to the SphK1 and forms several interactions with catalytically important residues of ATP-binding pocket. Adenosine Triphosphate 94-97 sphingosine kinase 1 Homo sapiens 16-21 31352240-10 2019 Taken together, our study suggests that EA can be utilized as a chemical prototype to develop potent therapeutics targeting SphK1-associated pathologies. Ellagic Acid 40-42 sphingosine kinase 1 Homo sapiens 124-129 31413745-0 2019 SphK1 functions downstream of IGF-1 to modulate IGF-1-induced EMT, migration and paclitaxel resistance of A549 cells: A preliminary in vitro study. Paclitaxel 81-91 sphingosine kinase 1 Homo sapiens 0-5 31632969-2 2019 Investigating the effects of various inhibitors of sphingolipid metabolism in primary human peripheral blood lymphocytes (PBL) and the human B cell line BJAB we found that not only the sphingosine kinase (SphK) inhibitor SKI-II, but also the acid ceramidase inhibitor ceranib-2 efficiently inhibited measles virus (MV) replication. N'-((2-hydroxy-1-naphthyl)methylene)-3-(2-naphthyl)-1H-pyrazole-5-carbohydrazide 221-227 sphingosine kinase 1 Homo sapiens 185-203 31632969-2 2019 Investigating the effects of various inhibitors of sphingolipid metabolism in primary human peripheral blood lymphocytes (PBL) and the human B cell line BJAB we found that not only the sphingosine kinase (SphK) inhibitor SKI-II, but also the acid ceramidase inhibitor ceranib-2 efficiently inhibited measles virus (MV) replication. N'-((2-hydroxy-1-naphthyl)methylene)-3-(2-naphthyl)-1H-pyrazole-5-carbohydrazide 221-227 sphingosine kinase 1 Homo sapiens 205-209 31737503-3 2019 Nevertheless, the roles of mRNA expression of major enzymes (SPHK1, SPHK2 and SGPL1) in S1P metabolism for predicting outcomes in NSCLC patients have not been determined. sphingosine 1-phosphate 88-91 sphingosine kinase 1 Homo sapiens 61-66 31128248-1 2019 Sphingosine kinases (SPHK) generate the sphingolipid sphingosine-1-phosphate, which, among other functions, is a potent regulator of inflammation. Sphingolipids 40-52 sphingosine kinase 1 Homo sapiens 0-19 31128248-1 2019 Sphingosine kinases (SPHK) generate the sphingolipid sphingosine-1-phosphate, which, among other functions, is a potent regulator of inflammation. Sphingolipids 40-52 sphingosine kinase 1 Homo sapiens 21-25 31128248-1 2019 Sphingosine kinases (SPHK) generate the sphingolipid sphingosine-1-phosphate, which, among other functions, is a potent regulator of inflammation. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 0-19 31128248-1 2019 Sphingosine kinases (SPHK) generate the sphingolipid sphingosine-1-phosphate, which, among other functions, is a potent regulator of inflammation. sphingosine 1-phosphate 53-76 sphingosine kinase 1 Homo sapiens 21-25 31363040-4 2019 Resistant cells, those selected for growth in Ara-C- and dnr-containing medium (HL-60/Ara-C and HL-60/dnr, respectively), demonstrated upregulated expression and activity of glucosylceramide synthase, acid ceramidase (AC), and sphingosine kinase 1 (SPHK1); were more resistant to ceramide than parental cells; and displayed sensitivity to inhibitors of SL metabolism. Cytarabine 46-51 sphingosine kinase 1 Homo sapiens 227-247 31363040-4 2019 Resistant cells, those selected for growth in Ara-C- and dnr-containing medium (HL-60/Ara-C and HL-60/dnr, respectively), demonstrated upregulated expression and activity of glucosylceramide synthase, acid ceramidase (AC), and sphingosine kinase 1 (SPHK1); were more resistant to ceramide than parental cells; and displayed sensitivity to inhibitors of SL metabolism. Cytarabine 46-51 sphingosine kinase 1 Homo sapiens 249-254 31220477-1 2019 Sphingosine kinase 1 (SK1) converts sphingosine to the bioactive lipid sphingosine 1-phosphate (S1P). Sphingosine 36-47 sphingosine kinase 1 Homo sapiens 0-20 31220477-1 2019 Sphingosine kinase 1 (SK1) converts sphingosine to the bioactive lipid sphingosine 1-phosphate (S1P). Sphingosine 36-47 sphingosine kinase 1 Homo sapiens 22-25 31220477-1 2019 Sphingosine kinase 1 (SK1) converts sphingosine to the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 71-94 sphingosine kinase 1 Homo sapiens 0-20 31220477-1 2019 Sphingosine kinase 1 (SK1) converts sphingosine to the bioactive lipid sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 71-94 sphingosine kinase 1 Homo sapiens 22-25 31265827-4 2019 In this work we report that concurrent treatment of human adenocarcinoma A549 cells with specific concentrations of two antitumor agents, the sphingosine kinase 1 inhibitor N, N dimethylsphingosine (DMS) and the alkylphosphocholine miltefosine, induced synergistic cytotoxic effect, which was confirmed by calculation of the combination index. N,N-dimethylsphingosine 173-197 sphingosine kinase 1 Homo sapiens 142-162 31265827-8 2019 Here we suggest a putative mechanism underlying the effect of miltefosine on sphingosine kinase 1, involving miltefosine-induced inhibition of protein kinase C. In conclusion, our findings provide a possibility for treatment of lung cancer cells with lower concentrations of the two antitumor drugs, DMS and miltefosine, which is favorable, regarding their potential cytotoxicity to normal cells. miltefosine 62-73 sphingosine kinase 1 Homo sapiens 77-97 31265827-8 2019 Here we suggest a putative mechanism underlying the effect of miltefosine on sphingosine kinase 1, involving miltefosine-induced inhibition of protein kinase C. In conclusion, our findings provide a possibility for treatment of lung cancer cells with lower concentrations of the two antitumor drugs, DMS and miltefosine, which is favorable, regarding their potential cytotoxicity to normal cells. miltefosine 109-120 sphingosine kinase 1 Homo sapiens 77-97 31265827-8 2019 Here we suggest a putative mechanism underlying the effect of miltefosine on sphingosine kinase 1, involving miltefosine-induced inhibition of protein kinase C. In conclusion, our findings provide a possibility for treatment of lung cancer cells with lower concentrations of the two antitumor drugs, DMS and miltefosine, which is favorable, regarding their potential cytotoxicity to normal cells. dms 300-303 sphingosine kinase 1 Homo sapiens 77-97 31265827-8 2019 Here we suggest a putative mechanism underlying the effect of miltefosine on sphingosine kinase 1, involving miltefosine-induced inhibition of protein kinase C. In conclusion, our findings provide a possibility for treatment of lung cancer cells with lower concentrations of the two antitumor drugs, DMS and miltefosine, which is favorable, regarding their potential cytotoxicity to normal cells. miltefosine 109-120 sphingosine kinase 1 Homo sapiens 77-97 31423186-0 2019 Sphingosine kinase-1 predicts overall survival outcomes in non-small cell lung cancer patients treated with carboplatin and navelbine. Carboplatin 108-119 sphingosine kinase 1 Homo sapiens 0-20 31423186-0 2019 Sphingosine kinase-1 predicts overall survival outcomes in non-small cell lung cancer patients treated with carboplatin and navelbine. Vinorelbine 124-133 sphingosine kinase 1 Homo sapiens 0-20 31423186-5 2019 Increased expression of SphK1 was significantly associated with shorter overall and disease free survival in patients treated with adjuvant platinum-based chemotherapy. Platinum 140-148 sphingosine kinase 1 Homo sapiens 24-29 31423186-7 2019 Collectively, the results suggest that the immunohistochemical detection of SphK1 may be a promising predictive marker in NSCLC patients treated with adjuvant platinum-based chemotherapy. Platinum 159-167 sphingosine kinase 1 Homo sapiens 76-81 31413745-6 2019 The results showed that, IGF-1 treatment of A549 cells stimulated the expression of SphK1, the activation of ERK and AKT, the cell migration, and the expression of EMT hallmark proteins, while inhibition of SphK1 by its specific inhibitor SKI-II suppressed all the above changes and increased the sensitivity of A549 cells to paclitaxel. Paclitaxel 326-336 sphingosine kinase 1 Homo sapiens 207-212 31413745-7 2019 Our data demonstrate that SphK1 acts as a downstream effector of IGF-1 and plays a critical role in IGF-1-induced EMT, cell migration and paclitaxel resistance of A549 cells, suggesting that SphK1 might be a potential therapeutic target for NSCLC. Paclitaxel 138-148 sphingosine kinase 1 Homo sapiens 26-31 31413745-7 2019 Our data demonstrate that SphK1 acts as a downstream effector of IGF-1 and plays a critical role in IGF-1-induced EMT, cell migration and paclitaxel resistance of A549 cells, suggesting that SphK1 might be a potential therapeutic target for NSCLC. Paclitaxel 138-148 sphingosine kinase 1 Homo sapiens 191-196 30889352-1 2019 Sphingosine kinase enzymes (SK1 and SK2) catalyze the conversion of sphingosine into sphingosine 1-phosphate and play a key role in lipid signaling and cellular responses. Sphingosine 68-79 sphingosine kinase 1 Homo sapiens 28-31 31101115-1 2019 BACKGROUND: Sphingosine kinase 1 (SPHK1) is an enzyme that converts pro-apoptotic ceramide and sphingosine into anti-apoptotic sphingosine-1-phosphate. Ceramides 82-90 sphingosine kinase 1 Homo sapiens 12-32 31101115-1 2019 BACKGROUND: Sphingosine kinase 1 (SPHK1) is an enzyme that converts pro-apoptotic ceramide and sphingosine into anti-apoptotic sphingosine-1-phosphate. Ceramides 82-90 sphingosine kinase 1 Homo sapiens 34-39 30753812-5 2019 Our results show that a chemical hypoxia mimetic cobalt chloride (CoCl2) increased SphK1 expression and activation in normal FLS but not in RAFLS. cobaltous chloride 49-64 sphingosine kinase 1 Homo sapiens 83-88 30753812-5 2019 Our results show that a chemical hypoxia mimetic cobalt chloride (CoCl2) increased SphK1 expression and activation in normal FLS but not in RAFLS. cobaltous chloride 66-71 sphingosine kinase 1 Homo sapiens 83-88 30753812-9 2019 The data suggest that altered expression and/or activation of SphK1 combined with reduced induction of S1P receptor expression by CoCl2 impaired the CoCl2-mediated autocrine S1P receptor signaling loop and chemokine production in RAFLS. cobaltous chloride 149-154 sphingosine kinase 1 Homo sapiens 62-67 30917625-1 2019 Sphingosine kinase 1 and its product, sphingosine 1-phosphate (S1P), as well as their receptors, have been implicated in inflammatory responses. sphingosine 1-phosphate 38-61 sphingosine kinase 1 Homo sapiens 0-20 31152147-7 2019 Specifically, LPS induced the S225 phosphorylation of SphK1 and the translocation of SphK1 to plasma membrane, leading to the production of sphingosine 1-phosphate (S1P), ERK1/2 and matriptase activation via S1P receptor 4 (S1PR4). sphingosine 1-phosphate 140-163 sphingosine kinase 1 Homo sapiens 54-59 31152147-7 2019 Specifically, LPS induced the S225 phosphorylation of SphK1 and the translocation of SphK1 to plasma membrane, leading to the production of sphingosine 1-phosphate (S1P), ERK1/2 and matriptase activation via S1P receptor 4 (S1PR4). sphingosine 1-phosphate 140-163 sphingosine kinase 1 Homo sapiens 85-90 30844311-3 2019 Sphingosine 1-phosphate (S1P) is a bioactive lipid mediator, generated by sphingosine kinases (SPHK1 and SPHK2), that mainly exerts its effects by binding to the following 5 GPCRs: S1P1 to S1P5. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 95-100 30456838-5 2019 Two sphingosine kinases, SphK1 and SphK2, convert sphingosine to sphingosine-1-phosphate, an antiapoptotic bioactive lipid mediator. Sphingosine 4-15 sphingosine kinase 1 Homo sapiens 25-30 30456838-5 2019 Two sphingosine kinases, SphK1 and SphK2, convert sphingosine to sphingosine-1-phosphate, an antiapoptotic bioactive lipid mediator. sphingosine 1-phosphate 65-88 sphingosine kinase 1 Homo sapiens 25-30 31101115-1 2019 BACKGROUND: Sphingosine kinase 1 (SPHK1) is an enzyme that converts pro-apoptotic ceramide and sphingosine into anti-apoptotic sphingosine-1-phosphate. Sphingosine 95-106 sphingosine kinase 1 Homo sapiens 12-32 31101115-1 2019 BACKGROUND: Sphingosine kinase 1 (SPHK1) is an enzyme that converts pro-apoptotic ceramide and sphingosine into anti-apoptotic sphingosine-1-phosphate. Sphingosine 95-106 sphingosine kinase 1 Homo sapiens 34-39 31101115-1 2019 BACKGROUND: Sphingosine kinase 1 (SPHK1) is an enzyme that converts pro-apoptotic ceramide and sphingosine into anti-apoptotic sphingosine-1-phosphate. sphingosine 1-phosphate 127-150 sphingosine kinase 1 Homo sapiens 12-32 31101115-1 2019 BACKGROUND: Sphingosine kinase 1 (SPHK1) is an enzyme that converts pro-apoptotic ceramide and sphingosine into anti-apoptotic sphingosine-1-phosphate. sphingosine 1-phosphate 127-150 sphingosine kinase 1 Homo sapiens 34-39 30669957-9 2019 Meanwhile, treatment of SPHK1-transfected cells with 3-methyladenine inhibited EMT. 3-methyladenine 53-68 sphingosine kinase 1 Homo sapiens 24-29 30889352-1 2019 Sphingosine kinase enzymes (SK1 and SK2) catalyze the conversion of sphingosine into sphingosine 1-phosphate and play a key role in lipid signaling and cellular responses. sphingosine 1-phosphate 85-108 sphingosine kinase 1 Homo sapiens 28-31 30889352-3 2019 We have probed these isoform differences with a ligand series derived from the potent SK1-selective inhibitor, PF-543. PF-543 111-117 sphingosine kinase 1 Homo sapiens 86-89 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Tetradecanoylphorbol Acetate 97-128 sphingosine kinase 1 Homo sapiens 163-168 30444039-0 2019 Translocation and activation of sphingosine kinase 1 by ceramide-1-phosphate. ceramide 1-phosphate 56-76 sphingosine kinase 1 Homo sapiens 32-52 30920672-0 2019 Involvement of calcium in 50-Hz magnetic field-induced activation of sphingosine kinase 1 signaling pathway. Calcium 15-22 sphingosine kinase 1 Homo sapiens 69-89 30920672-4 2019 Also, treatment with U0126, an inhibitor of ERK, could block MF-induced SK1 phosphorylation, but had no effect on PKCalpha phosphorylation. U 0126 21-26 sphingosine kinase 1 Homo sapiens 72-75 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Tetradecanoylphorbol Acetate 97-128 sphingosine kinase 1 Homo sapiens 163-168 30444039-3 2019 The translocation and activity of SphK1 are regulated by its phosphorylation of Ser 225 and by anionic lipids such as phosphatidic acid and phosphatidylserine. Serine 80-83 sphingosine kinase 1 Homo sapiens 34-39 30444039-3 2019 The translocation and activity of SphK1 are regulated by its phosphorylation of Ser 225 and by anionic lipids such as phosphatidic acid and phosphatidylserine. Phosphatidic Acids 118-135 sphingosine kinase 1 Homo sapiens 34-39 30444039-3 2019 The translocation and activity of SphK1 are regulated by its phosphorylation of Ser 225 and by anionic lipids such as phosphatidic acid and phosphatidylserine. Phosphatidylserines 140-158 sphingosine kinase 1 Homo sapiens 34-39 30444039-5 2019 C1P concentration dependently bound with and activated recombinant human SphK1. ceramide 1-phosphate 0-3 sphingosine kinase 1 Homo sapiens 73-78 30444039-6 2019 The inhibition of CerK reduced the phorbol 12-myristate 13-acetate-induced translocation of SphK1 to the plasma membrane (PM) and activation of the enzyme in membrane fractions of cells. Tetradecanoylphorbol Acetate 35-66 sphingosine kinase 1 Homo sapiens 92-97 30444039-7 2019 A treatment with C1P translocated wild-type SphK1, but not the SphK1-S225A mutant, to the PM without affecting phosphorylation signaling. ceramide 1-phosphate 17-20 sphingosine kinase 1 Homo sapiens 44-49 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. ceramide 1-phosphate 134-137 sphingosine kinase 1 Homo sapiens 79-84 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Alanine 45-48 sphingosine kinase 1 Homo sapiens 79-84 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Alanine 45-48 sphingosine kinase 1 Homo sapiens 163-168 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. ceramide 1-phosphate 134-137 sphingosine kinase 1 Homo sapiens 163-168 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Alanine 45-48 sphingosine kinase 1 Homo sapiens 163-168 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. Tetradecanoylphorbol Acetate 97-128 sphingosine kinase 1 Homo sapiens 79-84 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. ceramide 1-phosphate 134-137 sphingosine kinase 1 Homo sapiens 163-168 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. ceramide 1-phosphate 205-208 sphingosine kinase 1 Homo sapiens 79-84 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. ceramide 1-phosphate 205-208 sphingosine kinase 1 Homo sapiens 163-168 30444039-9 2019 The mutation of four cationic amino acids to Ala in the 56-RRNHAR-61 domain in SphK1 reduced the phorbol 12-myristate 13-acetate- and C1P-induced translocation of SphK1 to the PM, however, the capacity of C1P to bind with and activate SphK1 was not affected by this mutation. ceramide 1-phosphate 205-208 sphingosine kinase 1 Homo sapiens 163-168 30885289-5 2019 SPHK1 knockdown as well as inhibition of its enzymatic activity impeded the proliferation of hAD-SCs, with concomitant induction of cellular senescence and accumulation of sphingolipids, as seen in high-passage cells. Sphingolipids 172-185 sphingosine kinase 1 Homo sapiens 0-5 30655321-0 2019 SPHK1 Is a Novel Target of Metformin in Ovarian Cancer. Metformin 27-36 sphingosine kinase 1 Homo sapiens 0-5 30655321-9 2019 In support of this, metformin blocked hypoxia-induced SPHK1, which was associated with inhibited nuclear translocation and transcriptional activity of hypoxia-inducible factors (HIF1alpha and HIF2alpha). Metformin 20-29 sphingosine kinase 1 Homo sapiens 54-59 30655321-10 2019 Further, ovarian cancer cells with high SPHK1 were found to be highly sensitive to the cytotoxic effects of metformin, whereas ovarian cancer cells with low SPHK1 were resistant. Metformin 108-117 sphingosine kinase 1 Homo sapiens 40-45 30655321-11 2019 Together, the findings reported here show that hypoxia-induced SPHK1 expression and downstream S1P signaling promote ovarian cancer progression and that tumors with high expression of SPHK1 or S1P levels might have increased sensitivity to the cytotoxic effects of metformin. Metformin 265-274 sphingosine kinase 1 Homo sapiens 63-68 30655321-11 2019 Together, the findings reported here show that hypoxia-induced SPHK1 expression and downstream S1P signaling promote ovarian cancer progression and that tumors with high expression of SPHK1 or S1P levels might have increased sensitivity to the cytotoxic effects of metformin. Metformin 265-274 sphingosine kinase 1 Homo sapiens 184-189 30655321-12 2019 IMPLICATIONS: Metformin targets sphingolipid metabolism through inhibiting SPHK1, thereby impeding ovarian cancer cell migration, proliferation, and self-renewal. Metformin 14-23 sphingosine kinase 1 Homo sapiens 75-80 30655321-12 2019 IMPLICATIONS: Metformin targets sphingolipid metabolism through inhibiting SPHK1, thereby impeding ovarian cancer cell migration, proliferation, and self-renewal. Sphingolipids 32-44 sphingosine kinase 1 Homo sapiens 75-80 30885289-6 2019 SPHK1 knockdown-accelerated cellular senescence was attenuated by co-treatment with sphingosine-1-phosphate and an inhibitor of ceramide synthesis, fumonisin B1, but not by treatment with either one alone. sphingosine 1-phosphate 84-107 sphingosine kinase 1 Homo sapiens 0-5 30885289-6 2019 SPHK1 knockdown-accelerated cellular senescence was attenuated by co-treatment with sphingosine-1-phosphate and an inhibitor of ceramide synthesis, fumonisin B1, but not by treatment with either one alone. Ceramides 128-136 sphingosine kinase 1 Homo sapiens 0-5 30885289-6 2019 SPHK1 knockdown-accelerated cellular senescence was attenuated by co-treatment with sphingosine-1-phosphate and an inhibitor of ceramide synthesis, fumonisin B1, but not by treatment with either one alone. fumonisin B1 148-160 sphingosine kinase 1 Homo sapiens 0-5 30885289-7 2019 Together, these results suggest that transcriptional down-regulation of SPHK1 is a critical inducer of altered sphingolipid profiles and enhances replicative senescence during multiple rounds of cell division. Sphingolipids 111-123 sphingosine kinase 1 Homo sapiens 72-77 30655318-3 2019 Here, we show that sphingosine kinase (Sphk) activity was induced by the LXR agonist 22(R)-hydroxycholesterol and required for the stimulation of ABCA1-mediated cholesterol efflux to apolipoprotein A-I. 22-hydroxycholesterol 85-109 sphingosine kinase 1 Homo sapiens 19-37 30655318-3 2019 Here, we show that sphingosine kinase (Sphk) activity was induced by the LXR agonist 22(R)-hydroxycholesterol and required for the stimulation of ABCA1-mediated cholesterol efflux to apolipoprotein A-I. 22-hydroxycholesterol 85-109 sphingosine kinase 1 Homo sapiens 39-43 30655318-7 2019 The inability of excess exogenous S1P to further increase efflux was consistent with tonic S1P3 signaling by a pool of constitutively generated Sphk-derived S1P dynamically regulating cholesterol efflux. Cholesterol 184-195 sphingosine kinase 1 Homo sapiens 144-148 30726072-2 2019 In particular, 2 selectively targets the microRNA(miR)-515 hairpin precursor to inhibit production of miR-515 that represses sphingosine kinase 1 (SK1), a key enzyme in the biosynthesis of sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 189-212 sphingosine kinase 1 Homo sapiens 125-145 30726072-2 2019 In particular, 2 selectively targets the microRNA(miR)-515 hairpin precursor to inhibit production of miR-515 that represses sphingosine kinase 1 (SK1), a key enzyme in the biosynthesis of sphingosine 1-phosphate (S1P). sphingosine 1-phosphate 189-212 sphingosine kinase 1 Homo sapiens 147-150 30336275-8 2019 In contrast, SPHK1, S1P3 and S1P4 mRNA expression levels were upregulated by 1,25(OH)2D3 treatment compared to the respective controls. Calcitriol 77-88 sphingosine kinase 1 Homo sapiens 13-18 30336275-12 2019 Overall, our results demonstrate for the first time that 1,25(OH)2D3 treatment can influence S1P receptor and SPHK expression and S1P levels in primary monocytes of both HC and subjects with T2D. Calcitriol 57-68 sphingosine kinase 1 Homo sapiens 110-114 30527463-2 2019 Sphingosine-1-phosphate (S1P) is a pleiotropic bioactive lipid mediator, which produced by sphingosine kinase 1 (SphK1) activated by phosphorylation. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 91-111 30527463-2 2019 Sphingosine-1-phosphate (S1P) is a pleiotropic bioactive lipid mediator, which produced by sphingosine kinase 1 (SphK1) activated by phosphorylation. sphingosine 1-phosphate 0-23 sphingosine kinase 1 Homo sapiens 113-118 30250299-1 2019 While the two mammalian sphingosine kinases, SK1 and SK2, both catalyze the generation of pro-survival sphingosine 1-phosphate (S1P), their roles vary dependent on their different subcellular localization. sphingosine 1-phosphate 103-126 sphingosine kinase 1 Homo sapiens 45-48 30551451-0 2019 MiR-128 suppresses the growth of thyroid carcinoma by negatively regulating SPHK1. mir-128 0-7 sphingosine kinase 1 Homo sapiens 76-81 30551451-8 2019 Following, we characterized sphingosine kinase-1 (SPHK1) as a direct target of miR-128 that interacted with the 3"-untranslated region (UTR) of SPHK1, and the results were confirmed by using luciferase-reporter assay. mir-128 79-86 sphingosine kinase 1 Homo sapiens 28-48 30551451-8 2019 Following, we characterized sphingosine kinase-1 (SPHK1) as a direct target of miR-128 that interacted with the 3"-untranslated region (UTR) of SPHK1, and the results were confirmed by using luciferase-reporter assay. mir-128 79-86 sphingosine kinase 1 Homo sapiens 50-55 30551451-8 2019 Following, we characterized sphingosine kinase-1 (SPHK1) as a direct target of miR-128 that interacted with the 3"-untranslated region (UTR) of SPHK1, and the results were confirmed by using luciferase-reporter assay. mir-128 79-86 sphingosine kinase 1 Homo sapiens 144-149 30551451-9 2019 We also observed that SPHK1 expression was decreased and negatively correlated with miR-128 expression in PTC and FTC tissues clinically. mir-128 84-91 sphingosine kinase 1 Homo sapiens 22-27 30551451-10 2019 Importantly, ectopic expression of SPHK1 significantly abrogated the tumor-suppressive effect induced by miR-128, as supported by the reduced apoptosis, while the enhanced proliferation and metastasis. mir-128 105-112 sphingosine kinase 1 Homo sapiens 35-40 30551451-11 2019 Finally, over-expressing miR-128 apparently reduced the tumor growth rate and tumor weight in vivo using xenograft tumor model, accompanied with a remarkable decrease of SPHK1. mir-128 25-32 sphingosine kinase 1 Homo sapiens 170-175