PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 32142120-3 2020 WHAT IS KNOWN ALREADY: Sperm-specific PLCzeta is now widely considered to be the physiological stimulus that evokes intracellular calcium (Ca2+) oscillations, which are essential for egg activation during mammalian fertilization. Calcium 130-137 phospholipase C zeta 1 Homo sapiens 38-45 35038316-2 2022 At present, there is a general consensus that PLCZ1 is responsible for triggering the calcium (Ca2+) oscillations necessary to start the oocyte activation process in mammals. Calcium 86-93 phospholipase C zeta 1 Homo sapiens 46-51 35521907-2 2022 PLCzeta cRNA expression or direct protein microinjection into mammalian oocytes triggers calcium (Ca2+) oscillations indistinguishable from those observed at fertilization. Calcium 89-96 phospholipase C zeta 1 Homo sapiens 0-7 35521907-6 2022 The X and Y catalytic domains are responsible for the catalysis of the phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) substrate to produce the Ca2+-mobilising messenger, inositol 1,4,5-trisphosphate (IP3), while the XY linker plays multiple roles in the unique mode of PLCzeta action. Phosphatidylinositol 4,5-Diphosphate 71-108 phospholipase C zeta 1 Homo sapiens 272-279 35521907-6 2022 The X and Y catalytic domains are responsible for the catalysis of the phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) substrate to produce the Ca2+-mobilising messenger, inositol 1,4,5-trisphosphate (IP3), while the XY linker plays multiple roles in the unique mode of PLCzeta action. pi(4,5)p2 110-119 phospholipase C zeta 1 Homo sapiens 272-279 35521907-6 2022 The X and Y catalytic domains are responsible for the catalysis of the phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) substrate to produce the Ca2+-mobilising messenger, inositol 1,4,5-trisphosphate (IP3), while the XY linker plays multiple roles in the unique mode of PLCzeta action. Inositol 1,4,5-Trisphosphate 173-201 phospholipase C zeta 1 Homo sapiens 272-279 35521907-6 2022 The X and Y catalytic domains are responsible for the catalysis of the phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) substrate to produce the Ca2+-mobilising messenger, inositol 1,4,5-trisphosphate (IP3), while the XY linker plays multiple roles in the unique mode of PLCzeta action. Inositol 1,4,5-Trisphosphate 203-206 phospholipase C zeta 1 Homo sapiens 272-279 35521907-7 2022 Finally, the C2 domain has been proposed to facilitate the anchoring of PLCzeta to intracellular vesicles through its direct interactions with specific phosphoinositides. Phosphatidylinositols 152-169 phospholipase C zeta 1 Homo sapiens 72-79 32298520-1 2020 BACKGROUND: Oocyte activation is driven by intracellular calcium (Ca2+ ) oscillations induced by sperm specific PLCzeta, abrogation in which causes oocyte activation deficiency in humans. Calcium 57-64 phospholipase C zeta 1 Homo sapiens 112-119 32298520-11 2020 Finally, significantly higher levels of PLCzeta were exhibited by cases of fertilisation success, alongside higher proportions of Ac+Eq, and lower levels of dispersed PLCzeta. ac+eq 130-135 phospholipase C zeta 1 Homo sapiens 40-47 32285298-2 2020 In mammals, this process is initiated by a series of characteristic calcium (Ca2+) oscillations, induced by a sperm-specific phospholipase C (PLC) termed PLCzeta (PLCzeta). Calcium 68-75 phospholipase C zeta 1 Homo sapiens 154-161 32285298-2 2020 In mammals, this process is initiated by a series of characteristic calcium (Ca2+) oscillations, induced by a sperm-specific phospholipase C (PLC) termed PLCzeta (PLCzeta). Calcium 68-75 phospholipase C zeta 1 Homo sapiens 163-170 34893574-8 2021 The mean RF intensity of PLCzeta in AT- and HCl-treated spermatozoa from 12 infertile males was not significantly different from that of the non-AUM group. Hydrochloric Acid 44-47 phospholipase C zeta 1 Homo sapiens 25-32 35261925-2 2022 Oocyte activation encompasses a series of concerted events, triggered by sperm-specific phospholipase C zeta (PLCzeta), which elicits increases in free cytoplasmic calcium (Ca2+) in spatially and temporally specific oscillations. Calcium 164-171 phospholipase C zeta 1 Homo sapiens 88-108 35261925-2 2022 Oocyte activation encompasses a series of concerted events, triggered by sperm-specific phospholipase C zeta (PLCzeta), which elicits increases in free cytoplasmic calcium (Ca2+) in spatially and temporally specific oscillations. Calcium 164-171 phospholipase C zeta 1 Homo sapiens 110-117 33407934-5 2021 While PLCzeta mechanism is via IP3, how PAWP activates the oocyte still no clear, and these findings are important to study and treat fertilization failure due to oocyte activation, especially when one of the causes is the deficiency of PLCzeta in the sperm. Inositol 1,4,5-Trisphosphate 31-34 phospholipase C zeta 1 Homo sapiens 6-13 31232010-9 2019 The recombinant protein purified by Ni2+ affinity column was identified as PLCzeta by Western blotting and ionization time-of-flight mass spectrometry and the enzyme activity was up to 326.8 U/mL. Nickel(2+) 36-40 phospholipase C zeta 1 Homo sapiens 75-82 32064262-1 2020 Sperm-specific phospholipase C zeta (PLCzeta) is widely considered to be the physiological stimulus responsible for generating calcium (Ca2+) oscillations that induce egg activation and early embryonic development during mammalian fertilization. Calcium 127-134 phospholipase C zeta 1 Homo sapiens 37-44 32064262-1 2020 Sperm-specific phospholipase C zeta (PLCzeta) is widely considered to be the physiological stimulus responsible for generating calcium (Ca2+) oscillations that induce egg activation and early embryonic development during mammalian fertilization. Calcium 136-140 phospholipase C zeta 1 Homo sapiens 37-44 32064262-3 2020 Sperm-delivered PLCzeta induces Ca2+ release via the inositol 1,4,5-trisphosphate (InsP3) signaling pathway. Calcium 32-36 phospholipase C zeta 1 Homo sapiens 16-23 32064262-3 2020 Sperm-delivered PLCzeta induces Ca2+ release via the inositol 1,4,5-trisphosphate (InsP3) signaling pathway. Inositol 1,4,5-Trisphosphate 53-81 phospholipase C zeta 1 Homo sapiens 16-23 32064262-5 2020 However, the distinctive biochemical properties of PLCzeta compared with other PLC isoforms contribute to its unique potency in stimulating cytosolic Ca2+ oscillations within mammalian eggs. Calcium 150-154 phospholipase C zeta 1 Homo sapiens 51-58 32064262-8 2020 Herein, we give an update on recent advances that have refined our understanding of how sperm PLCzeta triggers Ca2 + oscillations and egg activation in mammals, while also discussing the nature of a potential "alternative" sperm factor. Calcium 111-114 phospholipase C zeta 1 Homo sapiens 94-101 32048714-3 2020 WHAT IS KNOWN ALREADY: PLCZ1 is essential for the induction of intracellular calcium (Ca2+) oscillations and the initiation of oocyte activation during mammalian fertilization. known 8-13 phospholipase C zeta 1 Homo sapiens 23-28 32048714-3 2020 WHAT IS KNOWN ALREADY: PLCZ1 is essential for the induction of intracellular calcium (Ca2+) oscillations and the initiation of oocyte activation during mammalian fertilization. Calcium 77-84 phospholipase C zeta 1 Homo sapiens 23-28 32048714-3 2020 WHAT IS KNOWN ALREADY: PLCZ1 is essential for the induction of intracellular calcium (Ca2+) oscillations and the initiation of oocyte activation during mammalian fertilization. Calcium 86-90 phospholipase C zeta 1 Homo sapiens 23-28 32219949-4 2020 Sperm-specific phospholipase C, i.e., PLC zeta (PLCzeta), is a strong candidate of the factor(s), and several research groups using different species obtained evidence that PLCzeta is a sperm factor that can induce calcium oscillations during fertilization. Calcium 215-222 phospholipase C zeta 1 Homo sapiens 38-46 32219949-4 2020 Sperm-specific phospholipase C, i.e., PLC zeta (PLCzeta), is a strong candidate of the factor(s), and several research groups using different species obtained evidence that PLCzeta is a sperm factor that can induce calcium oscillations during fertilization. Calcium 215-222 phospholipase C zeta 1 Homo sapiens 48-55 32219949-4 2020 Sperm-specific phospholipase C, i.e., PLC zeta (PLCzeta), is a strong candidate of the factor(s), and several research groups using different species obtained evidence that PLCzeta is a sperm factor that can induce calcium oscillations during fertilization. Calcium 215-222 phospholipase C zeta 1 Homo sapiens 173-180 31347677-4 2019 The sperm PLCzeta/PLCZ1 protein hydrolyzes phosphatidylinositol (4, 5)-bisphosphate in the oocyte, leading to intracellular calcium release and oocyte activation. Phosphatidylinositol 4,5-Diphosphate 43-83 phospholipase C zeta 1 Homo sapiens 10-17 31347677-4 2019 The sperm PLCzeta/PLCZ1 protein hydrolyzes phosphatidylinositol (4, 5)-bisphosphate in the oocyte, leading to intracellular calcium release and oocyte activation. Phosphatidylinositol 4,5-Diphosphate 43-83 phospholipase C zeta 1 Homo sapiens 18-23 31347677-4 2019 The sperm PLCzeta/PLCZ1 protein hydrolyzes phosphatidylinositol (4, 5)-bisphosphate in the oocyte, leading to intracellular calcium release and oocyte activation. Calcium 124-131 phospholipase C zeta 1 Homo sapiens 10-17 31347677-4 2019 The sperm PLCzeta/PLCZ1 protein hydrolyzes phosphatidylinositol (4, 5)-bisphosphate in the oocyte, leading to intracellular calcium release and oocyte activation. Calcium 124-131 phospholipase C zeta 1 Homo sapiens 18-23 29061915-4 2017 Sperm PLCzeta catalyses the hydrolysis of phosphatidylinositol 4,5-bisphosphate triggering cytosolic Ca2+ oscillations through the inositol 1,4,5-trisphosphate signalling pathway. Phosphatidylinositol 4,5-Diphosphate 42-79 phospholipase C zeta 1 Homo sapiens 6-13 30873668-3 2019 Several studies have introduced phospholipase Czeta (PLCzeta) as the main sperm factor inducing oocyte activation and thereby has the potential to act as a biomarker for the prediction of ICSI fertilisation outcome. czeta 46-51 phospholipase C zeta 1 Homo sapiens 53-60 29061915-4 2017 Sperm PLCzeta catalyses the hydrolysis of phosphatidylinositol 4,5-bisphosphate triggering cytosolic Ca2+ oscillations through the inositol 1,4,5-trisphosphate signalling pathway. Inositol 1,4,5-Trisphosphate 131-159 phospholipase C zeta 1 Homo sapiens 6-13 27932551-3 2017 WHAT IS KNOWN ALREADY: Mammalian oocyte activation is mediated via a series of intracellular calcium (Ca2+) oscillations induced by sperm-specific PLCzeta. Calcium 93-100 phospholipase C zeta 1 Homo sapiens 147-154 28270562-1 2017 Sperm-specific phospholipase C zeta (PLCzeta) is widely considered to be the physiological stimulus that evokes intracellular calcium (Ca2+) oscillations that are essential for the initiation of egg activation during mammalian fertilisation. Calcium 126-133 phospholipase C zeta 1 Homo sapiens 37-44 28270562-2 2017 A recent genetic study reported a male infertility case that was directly associated with a point mutation in the PLCzeta C2 domain, where an isoleucine residue had been substituted with a phenylalanine (I489F). Isoleucine 142-152 phospholipase C zeta 1 Homo sapiens 114-121 28270562-2 2017 A recent genetic study reported a male infertility case that was directly associated with a point mutation in the PLCzeta C2 domain, where an isoleucine residue had been substituted with a phenylalanine (I489F). Phenylalanine 189-202 phospholipase C zeta 1 Homo sapiens 114-121 28270562-6 2017 Our in vitro biochemical analysis suggested that the PLCzetaI489F mutant displayed similar enzymatic properties, but dramatically reduced binding to PI(3)P and PI(5)P-containing liposomes compared with wild-type PLCzeta. 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'D-myo-inositol-3'-phosphate) 149-155 phospholipase C zeta 1 Homo sapiens 53-60 28270562-6 2017 Our in vitro biochemical analysis suggested that the PLCzetaI489F mutant displayed similar enzymatic properties, but dramatically reduced binding to PI(3)P and PI(5)P-containing liposomes compared with wild-type PLCzeta. pi(5)p 160-166 phospholipase C zeta 1 Homo sapiens 53-60 27932551-13 2017 All methods of AUM on aldehyde-fixed sperm enhanced visualization efficacy for PLCzeta compared to visualization efficacy before AUM (P < 0.05 for all AUM interventions), but exerted no significant change upon PAWP or acrosin immunofluorescence following AUM. Aldehydes 22-30 phospholipase C zeta 1 Homo sapiens 79-86 27372854-3 2016 PLCzeta hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to produce diacylglycerol (DAG), which activates protein kinase C (PKC), and inositol 1,4,5-trisphosphate (IP3), which induces the release of Ca(2+) from endoplasmic reticulum (ER) Ca(2+) stores. Phosphatidylinositol 4,5-Diphosphate 19-56 phospholipase C zeta 1 Homo sapiens 0-7 27089467-10 2016 High fertilization (56.06%) and pregnancy (41.7%) rates accomplished in this study following ICSI-AOA indicated that expression profiles of PLCzeta, PAWP, and TR-KIT were low in globozoospermic individuals, and ICSI combined with artificial oocyte activation could mimic physiological calcium changes which occur during fertilization. Calcium 285-292 phospholipase C zeta 1 Homo sapiens 140-147 27372854-3 2016 PLCzeta hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to produce diacylglycerol (DAG), which activates protein kinase C (PKC), and inositol 1,4,5-trisphosphate (IP3), which induces the release of Ca(2+) from endoplasmic reticulum (ER) Ca(2+) stores. Phosphatidylinositol 4,5-Diphosphate 58-62 phospholipase C zeta 1 Homo sapiens 0-7 27372854-3 2016 PLCzeta hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to produce diacylglycerol (DAG), which activates protein kinase C (PKC), and inositol 1,4,5-trisphosphate (IP3), which induces the release of Ca(2+) from endoplasmic reticulum (ER) Ca(2+) stores. Diglycerides 75-89 phospholipase C zeta 1 Homo sapiens 0-7 27372854-3 2016 PLCzeta hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to produce diacylglycerol (DAG), which activates protein kinase C (PKC), and inositol 1,4,5-trisphosphate (IP3), which induces the release of Ca(2+) from endoplasmic reticulum (ER) Ca(2+) stores. Diglycerides 91-94 phospholipase C zeta 1 Homo sapiens 0-7 27372854-3 2016 PLCzeta hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to produce diacylglycerol (DAG), which activates protein kinase C (PKC), and inositol 1,4,5-trisphosphate (IP3), which induces the release of Ca(2+) from endoplasmic reticulum (ER) Ca(2+) stores. Inositol 1,4,5-Trisphosphate 141-169 phospholipase C zeta 1 Homo sapiens 0-7 27372854-3 2016 PLCzeta hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to produce diacylglycerol (DAG), which activates protein kinase C (PKC), and inositol 1,4,5-trisphosphate (IP3), which induces the release of Ca(2+) from endoplasmic reticulum (ER) Ca(2+) stores. Inositol 1,4,5-Trisphosphate 171-174 phospholipase C zeta 1 Homo sapiens 0-7 26862214-7 2016 We do not yet understand exactly how PLCzeta causes inositol 1,4,5-trisphosphate (InsP3) production in eggs. Inositol 1,4,5-Trisphosphate 52-80 phospholipase C zeta 1 Homo sapiens 37-44 26780328-6 2016 Mounting evidence now indicates that these calcium oscillations are caused by a testis-specific PLC termed PLCzeta, released into the oocyte following gamete fusion. Calcium 43-50 phospholipase C zeta 1 Homo sapiens 107-114 26862214-8 2016 Sperm PLCzeta is distinct among mammalian PI-specific PLCs in that it is far more potent in triggering Ca(2+) oscillations in eggs than other PLCs, but it lacks a PH domain that would otherwise be considered essential for binding to the phosphatidylinositol 4,5-bisphosphate (PIP2) substrate. Phosphatidylinositol 4,5-Diphosphate 237-274 phospholipase C zeta 1 Homo sapiens 6-13 26862214-8 2016 Sperm PLCzeta is distinct among mammalian PI-specific PLCs in that it is far more potent in triggering Ca(2+) oscillations in eggs than other PLCs, but it lacks a PH domain that would otherwise be considered essential for binding to the phosphatidylinositol 4,5-bisphosphate (PIP2) substrate. Phosphatidylinositol 4,5-Diphosphate 276-280 phospholipase C zeta 1 Homo sapiens 6-13 26862214-10 2016 We consider how other regions of PLCzeta may mediate its binding to PIP2 in eggs and how interaction of PLCzeta with egg-specific factors could enable the hydrolysis of internal sources of PIP2. Phosphatidylinositol 4,5-Diphosphate 68-72 phospholipase C zeta 1 Homo sapiens 33-40 26453398-7 2016 Much evidence now indicates that the sperm-derived factor is phospholipase C-zeta (PLCzeta) that cleaves PIP2 and generates IP3, eventually leading to oocyte activation. Phosphatidylinositol 4,5-Diphosphate 105-109 phospholipase C zeta 1 Homo sapiens 61-81 26453398-7 2016 Much evidence now indicates that the sperm-derived factor is phospholipase C-zeta (PLCzeta) that cleaves PIP2 and generates IP3, eventually leading to oocyte activation. Phosphatidylinositol 4,5-Diphosphate 105-109 phospholipase C zeta 1 Homo sapiens 83-90 26453398-7 2016 Much evidence now indicates that the sperm-derived factor is phospholipase C-zeta (PLCzeta) that cleaves PIP2 and generates IP3, eventually leading to oocyte activation. Inositol 1,4,5-Trisphosphate 124-127 phospholipase C zeta 1 Homo sapiens 61-81 26453398-7 2016 Much evidence now indicates that the sperm-derived factor is phospholipase C-zeta (PLCzeta) that cleaves PIP2 and generates IP3, eventually leading to oocyte activation. Inositol 1,4,5-Trisphosphate 124-127 phospholipase C zeta 1 Homo sapiens 83-90 26631595-7 2016 The strongest empirical studies to date suggest that mammalian sperm-triggered Ca(2+) oscillations are caused by the introduction of a sperm-specific protein, called phospholipase C-zeta (PLCzeta) that generates inositol trisphosphate within the egg. inositol 1,2,3-trisphosphate 212-234 phospholipase C zeta 1 Homo sapiens 166-186 26631595-7 2016 The strongest empirical studies to date suggest that mammalian sperm-triggered Ca(2+) oscillations are caused by the introduction of a sperm-specific protein, called phospholipase C-zeta (PLCzeta) that generates inositol trisphosphate within the egg. inositol 1,2,3-trisphosphate 212-234 phospholipase C zeta 1 Homo sapiens 188-195 26347343-8 2015 CONCLUSIONS: Oocyte activation requires the triggering of intracellular calcium oscillations by the release of a sperm-specific factor (phospholipase C zeta (PLCzeta)) into the oocyte cytoplasm. Calcium 72-79 phospholipase C zeta 1 Homo sapiens 136-166 26009178-9 2015 In contrast, PLCzeta appears to interact with intracellular vesicles in eggs that contain PIP2. Phosphatidylinositol 4,5-Diphosphate 90-94 phospholipase C zeta 1 Homo sapiens 13-20 23916605-6 2013 Evidence suggests that these processes are initiated and controlled by calcium release from ooplasmic sources in response to PLCzeta activity via the inositol-1,4,5-triphosphate (IP3) pathway. Calcium 71-78 phospholipase C zeta 1 Homo sapiens 125-132 25057041-3 2014 Microinjection of the sperm-derived phospholipase C-zeta (PLCzeta), which generates IP3, causes the same pattern of Ca(2+) oscillations as observed at mammalian fertilization and it is thought to be the physiological agent that triggers oocyte activation. Inositol 1,4,5-Trisphosphate 84-87 phospholipase C zeta 1 Homo sapiens 36-56 25057041-3 2014 Microinjection of the sperm-derived phospholipase C-zeta (PLCzeta), which generates IP3, causes the same pattern of Ca(2+) oscillations as observed at mammalian fertilization and it is thought to be the physiological agent that triggers oocyte activation. Inositol 1,4,5-Trisphosphate 84-87 phospholipase C zeta 1 Homo sapiens 58-65 26023673-7 2015 However, immunoreactivity of PLCzeta showed a significant negative relationship with 8-OHdG immunoreactivity (r=-0.404, P=0.009). 8-ohdg 85-91 phospholipase C zeta 1 Homo sapiens 29-36 24152875-8 2013 Notably, chimeras containing the PLCzeta XY-linker bound to PIP2-containing liposomes, while chimeras containing the PLCzeta C2 domain exhibited PI(3)P binding. Phosphatidylinositol 4,5-Diphosphate 60-64 phospholipase C zeta 1 Homo sapiens 33-40 24152875-8 2013 Notably, chimeras containing the PLCzeta XY-linker bound to PIP2-containing liposomes, while chimeras containing the PLCzeta C2 domain exhibited PI(3)P binding. (3)p 147-151 phospholipase C zeta 1 Homo sapiens 117-124 24152875-9 2013 Our data suggest that the EF hands are not solely responsible for the nanomolar Ca(2+) sensitivity of PLCzeta and that membrane PIP2 binding involves the C2 domain and XY-linker of PLCzeta. Phosphatidylinositol 4,5-Diphosphate 128-132 phospholipase C zeta 1 Homo sapiens 181-188 24157362-1 2013 Significant evidence now supports the assertion that cytosolic calcium oscillations during fertilization in mammalian eggs are mediated by a testis-specific phospholipase C (PLC), termed PLC-zeta (PLCzeta) that is released into the egg following gamete fusion. Calcium 63-70 phospholipase C zeta 1 Homo sapiens 187-195 24157362-1 2013 Significant evidence now supports the assertion that cytosolic calcium oscillations during fertilization in mammalian eggs are mediated by a testis-specific phospholipase C (PLC), termed PLC-zeta (PLCzeta) that is released into the egg following gamete fusion. Calcium 63-70 phospholipase C zeta 1 Homo sapiens 197-204 24157362-3 2013 We describe the data suggesting that PLCzeta has distinct features amongst PLCs enabling the hydrolysis of its substrate, phosphatidylinositol 4,5-bisphosphate (PIP2) at low Ca(2+) levels. Phosphatidylinositol 4,5-Diphosphate 122-159 phospholipase C zeta 1 Homo sapiens 37-44 24157362-3 2013 We describe the data suggesting that PLCzeta has distinct features amongst PLCs enabling the hydrolysis of its substrate, phosphatidylinositol 4,5-bisphosphate (PIP2) at low Ca(2+) levels. Phosphatidylinositol 4,5-Diphosphate 161-165 phospholipase C zeta 1 Homo sapiens 37-44 24157362-4 2013 PLCzeta appears to be unique in its ability to target PIP2 that is present on intracellular vesicles. Phosphatidylinositol 4,5-Diphosphate 54-58 phospholipase C zeta 1 Homo sapiens 0-7 23916605-6 2013 Evidence suggests that these processes are initiated and controlled by calcium release from ooplasmic sources in response to PLCzeta activity via the inositol-1,4,5-triphosphate (IP3) pathway. Inositol 1,4,5-Trisphosphate 150-177 phospholipase C zeta 1 Homo sapiens 125-132 23916605-6 2013 Evidence suggests that these processes are initiated and controlled by calcium release from ooplasmic sources in response to PLCzeta activity via the inositol-1,4,5-triphosphate (IP3) pathway. Inositol 1,4,5-Trisphosphate 179-182 phospholipase C zeta 1 Homo sapiens 125-132 23218672-8 2013 We also discuss the evidence that PLCzeta does not hydrolyze the conventional source of PI(4,5)P(2) in the plasma membrane to make InsP(3), but instead uses a distinct pool of PI(4,5)P(2) present on intracellular vesicles. pi(4,5)p 88-96 phospholipase C zeta 1 Homo sapiens 34-41 23218672-8 2013 We also discuss the evidence that PLCzeta does not hydrolyze the conventional source of PI(4,5)P(2) in the plasma membrane to make InsP(3), but instead uses a distinct pool of PI(4,5)P(2) present on intracellular vesicles. pi(4,5)p 176-184 phospholipase C zeta 1 Homo sapiens 34-41 22633260-1 2012 OBJECTIVE: To examine the underlying factors leading to infertility in a male patient from whom phospholipase C zeta H398P (PLCzeta(H398P), histidine > proline) and PLCzeta(H233L) (histidine > leucine) mutations were previously identified. Histidine 184-193 phospholipase C zeta 1 Homo sapiens 96-116 22633260-1 2012 OBJECTIVE: To examine the underlying factors leading to infertility in a male patient from whom phospholipase C zeta H398P (PLCzeta(H398P), histidine > proline) and PLCzeta(H233L) (histidine > leucine) mutations were previously identified. Leucine 199-206 phospholipase C zeta 1 Homo sapiens 96-116 21767260-3 2011 Unlike for somatic PLCs, the absence of the PLCzeta XY-linker significantly diminishes both in vitro PIP2 (phosphatidylinositol 4,5-bisphosphate) hydrolysis and in vivo Ca2+-oscillation-inducing activity, revealing evidence for a novel PLCzeta enzymatic mechanism. Phosphatidylinositol 4,5-Diphosphate 101-105 phospholipase C zeta 1 Homo sapiens 44-51 22453985-3 2012 Compelling evidence now indicates that these calcium oscillations are caused by a testis-specific phospholipase C (PLC) termed PLCzeta (PLCzeta), released into the oocyte following gamete fusion. Calcium 45-52 phospholipase C zeta 1 Homo sapiens 127-134 22453985-3 2012 Compelling evidence now indicates that these calcium oscillations are caused by a testis-specific phospholipase C (PLC) termed PLCzeta (PLCzeta), released into the oocyte following gamete fusion. Calcium 45-52 phospholipase C zeta 1 Homo sapiens 136-143 22114355-9 2012 In contrast to somatic PLCs, our data indicate that sperm PLCzeta induces Ca(2+) mobilization by hydrolyzing internal PI(4,5)P(2) stores, suggesting that the mechanism of mammalian fertilization comprises a novel phosphoinositide signaling pathway. pi(4,5)p(2) 118-129 phospholipase C zeta 1 Homo sapiens 58-65 22114355-9 2012 In contrast to somatic PLCs, our data indicate that sperm PLCzeta induces Ca(2+) mobilization by hydrolyzing internal PI(4,5)P(2) stores, suggesting that the mechanism of mammalian fertilization comprises a novel phosphoinositide signaling pathway. Phosphatidylinositols 213-229 phospholipase C zeta 1 Homo sapiens 58-65 22456923-0 2012 Recombinant human phospholipase C zeta 1 induces intracellular calcium oscillations and oocyte activation in mouse and human oocytes. Calcium 63-70 phospholipase C zeta 1 Homo sapiens 18-40 22456923-5 2012 METHODS: Recombinant hPLCZ1 was synthesized using the Escherichia coli system, and subjected to immunoblot analysis with anti-PLCZ1 and anti-His tag antibodies. Histidine 141-144 phospholipase C zeta 1 Homo sapiens 21-27 22456923-5 2012 METHODS: Recombinant hPLCZ1 was synthesized using the Escherichia coli system, and subjected to immunoblot analysis with anti-PLCZ1 and anti-His tag antibodies. Histidine 141-144 phospholipase C zeta 1 Homo sapiens 22-27 22456923-11 2012 U73122, a PLC inhibitor, blocked the ability of hPLCZ1 to initiate oscillations. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 0-6 phospholipase C zeta 1 Homo sapiens 48-54 22086556-5 2012 Sperm PLCzeta effects Ca(2+) release from egg intracellular stores by hydrolyzing the membrane lipid PIP(2) and consequent stimulation of the inositol 1,4,5-trisphosphate (InsP(3) ) receptor Ca(2+) -signalling pathway, leading to egg activation and early embryogenesis. piperidine 102-105 phospholipase C zeta 1 Homo sapiens 6-13 22086556-5 2012 Sperm PLCzeta effects Ca(2+) release from egg intracellular stores by hydrolyzing the membrane lipid PIP(2) and consequent stimulation of the inositol 1,4,5-trisphosphate (InsP(3) ) receptor Ca(2+) -signalling pathway, leading to egg activation and early embryogenesis. Inositol 144-152 phospholipase C zeta 1 Homo sapiens 6-13 22086556-5 2012 Sperm PLCzeta effects Ca(2+) release from egg intracellular stores by hydrolyzing the membrane lipid PIP(2) and consequent stimulation of the inositol 1,4,5-trisphosphate (InsP(3) ) receptor Ca(2+) -signalling pathway, leading to egg activation and early embryogenesis. ,4,5-trisphosphate 154-172 phospholipase C zeta 1 Homo sapiens 6-13 22095789-2 2012 Certain types of male infertility are caused by failure of the sperm to activate the oocyte, a process normally regulated by calcium oscillations, thought to be induced by a sperm-specific phospholipase C, PLCzeta (PLCzeta). Calcium 125-132 phospholipase C zeta 1 Homo sapiens 206-213 22095789-2 2012 Certain types of male infertility are caused by failure of the sperm to activate the oocyte, a process normally regulated by calcium oscillations, thought to be induced by a sperm-specific phospholipase C, PLCzeta (PLCzeta). Calcium 125-132 phospholipase C zeta 1 Homo sapiens 215-222 22095789-4 2012 METHODS AND RESULTS: Here, using a combination of direct-sequencing and mini-sequencing of the PLCzeta gene from the patient and his family, we report the identification of a second PLCzeta mutation in the same patient resulting in a histidine to leucine substitution at position 233 (PLCzeta(H233L)), which is predicted to disrupt local protein interactions in a manner similar to PLCzeta(H398P) and was shown to exhibit abnormal calcium oscillatory ability following predictive 3D modelling and cRNA injection in mouse oocytes respectively. Calcium 431-438 phospholipase C zeta 1 Homo sapiens 95-102 22095789-4 2012 METHODS AND RESULTS: Here, using a combination of direct-sequencing and mini-sequencing of the PLCzeta gene from the patient and his family, we report the identification of a second PLCzeta mutation in the same patient resulting in a histidine to leucine substitution at position 233 (PLCzeta(H233L)), which is predicted to disrupt local protein interactions in a manner similar to PLCzeta(H398P) and was shown to exhibit abnormal calcium oscillatory ability following predictive 3D modelling and cRNA injection in mouse oocytes respectively. Calcium 431-438 phospholipase C zeta 1 Homo sapiens 182-189 22095789-4 2012 METHODS AND RESULTS: Here, using a combination of direct-sequencing and mini-sequencing of the PLCzeta gene from the patient and his family, we report the identification of a second PLCzeta mutation in the same patient resulting in a histidine to leucine substitution at position 233 (PLCzeta(H233L)), which is predicted to disrupt local protein interactions in a manner similar to PLCzeta(H398P) and was shown to exhibit abnormal calcium oscillatory ability following predictive 3D modelling and cRNA injection in mouse oocytes respectively. Calcium 431-438 phospholipase C zeta 1 Homo sapiens 182-189 22095789-4 2012 METHODS AND RESULTS: Here, using a combination of direct-sequencing and mini-sequencing of the PLCzeta gene from the patient and his family, we report the identification of a second PLCzeta mutation in the same patient resulting in a histidine to leucine substitution at position 233 (PLCzeta(H233L)), which is predicted to disrupt local protein interactions in a manner similar to PLCzeta(H398P) and was shown to exhibit abnormal calcium oscillatory ability following predictive 3D modelling and cRNA injection in mouse oocytes respectively. Calcium 431-438 phospholipase C zeta 1 Homo sapiens 182-189 22010140-0 2011 Loss of activity mutations in phospholipase C zeta (PLCzeta) abolishes calcium oscillatory ability of human recombinant protein in mouse oocytes. Calcium 71-78 phospholipase C zeta 1 Homo sapiens 52-59 22010140-1 2011 BACKGROUND: Mammalian oocyte activation occurs via a series of intracellular calcium (Ca(2+)) oscillations thought to be induced by a sperm-specific phospholipase C zeta (PLCzeta). Calcium 77-84 phospholipase C zeta 1 Homo sapiens 171-178 22010140-3 2011 Molecular analysis of the PLCzeta gene of a male patient with oocyte activation deficiency has previously identified a point mutation causing a histidine to proline substitution at PLCzeta residue 398 (PLCzeta(H398P)), leading to abnormal Ca(2+) release profiles and reduced oocyte activation efficiency. Histidine 144-153 phospholipase C zeta 1 Homo sapiens 26-33 22010140-3 2011 Molecular analysis of the PLCzeta gene of a male patient with oocyte activation deficiency has previously identified a point mutation causing a histidine to proline substitution at PLCzeta residue 398 (PLCzeta(H398P)), leading to abnormal Ca(2+) release profiles and reduced oocyte activation efficiency. Histidine 144-153 phospholipase C zeta 1 Homo sapiens 181-188 22010140-3 2011 Molecular analysis of the PLCzeta gene of a male patient with oocyte activation deficiency has previously identified a point mutation causing a histidine to proline substitution at PLCzeta residue 398 (PLCzeta(H398P)), leading to abnormal Ca(2+) release profiles and reduced oocyte activation efficiency. Histidine 144-153 phospholipase C zeta 1 Homo sapiens 181-188 22010140-3 2011 Molecular analysis of the PLCzeta gene of a male patient with oocyte activation deficiency has previously identified a point mutation causing a histidine to proline substitution at PLCzeta residue 398 (PLCzeta(H398P)), leading to abnormal Ca(2+) release profiles and reduced oocyte activation efficiency. Proline 157-164 phospholipase C zeta 1 Homo sapiens 26-33 22010140-3 2011 Molecular analysis of the PLCzeta gene of a male patient with oocyte activation deficiency has previously identified a point mutation causing a histidine to proline substitution at PLCzeta residue 398 (PLCzeta(H398P)), leading to abnormal Ca(2+) release profiles and reduced oocyte activation efficiency. Proline 157-164 phospholipase C zeta 1 Homo sapiens 181-188 22010140-3 2011 Molecular analysis of the PLCzeta gene of a male patient with oocyte activation deficiency has previously identified a point mutation causing a histidine to proline substitution at PLCzeta residue 398 (PLCzeta(H398P)), leading to abnormal Ca(2+) release profiles and reduced oocyte activation efficiency. Proline 157-164 phospholipase C zeta 1 Homo sapiens 181-188 21692749-1 2011 Sperm PLCzeta (phospholipase Czeta) is a distinct phosphoinositide-specific PLC isoform that is proposed to be the physiological trigger of egg activation and embryo development at mammalian fertilization. Phosphatidylinositols 50-66 phospholipase C zeta 1 Homo sapiens 6-13 21692749-7 2011 Furthermore, either PLCzeta-transfected CHO cells or derived cell extracts could specifically cause cytoplasmic Ca2+ oscillations when microinjected into mouse eggs. cho 40-43 phospholipase C zeta 1 Homo sapiens 20-27 21767260-3 2011 Unlike for somatic PLCs, the absence of the PLCzeta XY-linker significantly diminishes both in vitro PIP2 (phosphatidylinositol 4,5-bisphosphate) hydrolysis and in vivo Ca2+-oscillation-inducing activity, revealing evidence for a novel PLCzeta enzymatic mechanism. Phosphatidylinositol 4,5-Diphosphate 107-144 phospholipase C zeta 1 Homo sapiens 44-51 21204786-1 2011 A male infertility-linked human PLCzeta (phospholipase Czeta) mutation introduced into mouse PLCzeta completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca2+ oscillations in mouse eggs. Phosphatidylinositol 4,5-Diphosphate 136-142 phospholipase C zeta 1 Homo sapiens 32-60 21665540-3 2011 DGW significantly elevated the proportion of spermatozoa in which PLCzeta could be detected (by 25-81%; P < 0.0001). DGW 0-3 phospholipase C zeta 1 Homo sapiens 66-73 21204786-1 2011 A male infertility-linked human PLCzeta (phospholipase Czeta) mutation introduced into mouse PLCzeta completely abolishes both in vitro PIP(2) (phosphatidylinositol 4,5-bisphosphate) hydrolysis activity and the ability to trigger in vivo Ca2+ oscillations in mouse eggs. Phosphatidylinositol 4,5-Diphosphate 144-181 phospholipase C zeta 1 Homo sapiens 32-60 18471090-3 2008 The specific phosphatidylinositol 4,5-bisphosphate hydrolytic activity of PLCzeta implies that DAG (diacylglycerol) production, and hence PKC (protein kinase C) stimulation, also occurs during mammalian egg fertilization. Phosphatidylinositol 4,5-Diphosphate 13-50 phospholipase C zeta 1 Homo sapiens 74-81 19252507-5 2009 The absence of HCO3-, a key factor in activating capacitation, from the capacitation-inducing medium prevents the loss of NYD-SP27 from sperm. Bicarbonates 15-19 phospholipase C zeta 1 Homo sapiens 122-130 19252507-6 2009 The anti-NYD-SP27 antibody also prevents the loss of NYD-SP27 from sperm, reduced the number of capacitated sperm, inhibited the acrosome reaction induced by ATP and progesterone, and inhibited agonist-induced PLC-coupled Ca2+ mobilization in sperm, which can be mimicked by the PLC inhibitor, U73122. Adenosine Triphosphate 158-161 phospholipase C zeta 1 Homo sapiens 9-17 19252507-6 2009 The anti-NYD-SP27 antibody also prevents the loss of NYD-SP27 from sperm, reduced the number of capacitated sperm, inhibited the acrosome reaction induced by ATP and progesterone, and inhibited agonist-induced PLC-coupled Ca2+ mobilization in sperm, which can be mimicked by the PLC inhibitor, U73122. Progesterone 166-178 phospholipase C zeta 1 Homo sapiens 9-17 19252507-6 2009 The anti-NYD-SP27 antibody also prevents the loss of NYD-SP27 from sperm, reduced the number of capacitated sperm, inhibited the acrosome reaction induced by ATP and progesterone, and inhibited agonist-induced PLC-coupled Ca2+ mobilization in sperm, which can be mimicked by the PLC inhibitor, U73122. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 294-300 phospholipase C zeta 1 Homo sapiens 9-17 20357268-1 2010 Injection of mammalian sperm extracts or cRNA of the sperm-specific phospholipase C zeta 1 (PLCZ1) has been shown to trigger repetitive oscillations in the concentration of free calcium ([Ca(2+)](i)), leading to oocyte activation and embryo development in all mammals studied to date. Calcium 178-185 phospholipase C zeta 1 Homo sapiens 68-90 20357268-1 2010 Injection of mammalian sperm extracts or cRNA of the sperm-specific phospholipase C zeta 1 (PLCZ1) has been shown to trigger repetitive oscillations in the concentration of free calcium ([Ca(2+)](i)), leading to oocyte activation and embryo development in all mammals studied to date. Calcium 178-185 phospholipase C zeta 1 Homo sapiens 92-97 20378416-4 2010 One likely candidate, phospholipase C (PLC) zeta 1, induces calcium release in mammalian oocytes; however, work on other deuterostomes suggests that the sperm factor is non-specific and multifactorial, regulating several activation events. Calcium 60-67 phospholipase C zeta 1 Homo sapiens 22-50 18471090-3 2008 The specific phosphatidylinositol 4,5-bisphosphate hydrolytic activity of PLCzeta implies that DAG (diacylglycerol) production, and hence PKC (protein kinase C) stimulation, also occurs during mammalian egg fertilization. Diglycerides 95-98 phospholipase C zeta 1 Homo sapiens 74-81 18471090-3 2008 The specific phosphatidylinositol 4,5-bisphosphate hydrolytic activity of PLCzeta implies that DAG (diacylglycerol) production, and hence PKC (protein kinase C) stimulation, also occurs during mammalian egg fertilization. Diglycerides 100-114 phospholipase C zeta 1 Homo sapiens 74-81 18322275-6 2008 Mouse and human PLCZ1 translocated into the nucleus of COS-7 cells transfected with cDNA. carbonyl sulfide 55-58 phospholipase C zeta 1 Homo sapiens 16-21 18649272-3 2008 PLCzeta is stimulated by low intracellular calcium levels in a manner which suggests that there is a regenerative feedback of calcium release and PLCzeta induced inositol 1,4,5-trisphophate (InsP3) production in eggs. Calcium 43-50 phospholipase C zeta 1 Homo sapiens 0-7 18649272-3 2008 PLCzeta is stimulated by low intracellular calcium levels in a manner which suggests that there is a regenerative feedback of calcium release and PLCzeta induced inositol 1,4,5-trisphophate (InsP3) production in eggs. Calcium 126-133 phospholipase C zeta 1 Homo sapiens 0-7 18649272-3 2008 PLCzeta is stimulated by low intracellular calcium levels in a manner which suggests that there is a regenerative feedback of calcium release and PLCzeta induced inositol 1,4,5-trisphophate (InsP3) production in eggs. inositol 1,4,5-trisphophate 162-189 phospholipase C zeta 1 Homo sapiens 0-7 18649272-3 2008 PLCzeta is stimulated by low intracellular calcium levels in a manner which suggests that there is a regenerative feedback of calcium release and PLCzeta induced inositol 1,4,5-trisphophate (InsP3) production in eggs. inositol 1,4,5-trisphophate 162-189 phospholipase C zeta 1 Homo sapiens 146-153 17430887-0 2007 Binding of phosphoinositide-specific phospholipase C-zeta (PLC-zeta) to phospholipid membranes: potential role of an unstructured cluster of basic residues. Phosphatidylinositols 11-27 phospholipase C zeta 1 Homo sapiens 37-57 17430887-8 2007 Expressed fragments corresponding to the PLC-zeta X-Y linker region also bind with higher affinity to polyvalent than monovalent phosphoinositides on nitrocellulose filters. Phosphatidylinositols 129-146 phospholipase C zeta 1 Homo sapiens 41-49 17430887-0 2007 Binding of phosphoinositide-specific phospholipase C-zeta (PLC-zeta) to phospholipid membranes: potential role of an unstructured cluster of basic residues. Phosphatidylinositols 11-27 phospholipase C zeta 1 Homo sapiens 59-67 17430887-9 2007 A peptide corresponding to the basic cluster (charge=+7) within the linker region, PLC-zeta-(374-385), binds to PC/PS vesicles with higher affinity than PLC-zeta, but its binding is less sensitive to incorporating PIP2. Phosphatidylinositol 4,5-Diphosphate 214-218 phospholipase C zeta 1 Homo sapiens 83-91 17430887-0 2007 Binding of phosphoinositide-specific phospholipase C-zeta (PLC-zeta) to phospholipid membranes: potential role of an unstructured cluster of basic residues. Phospholipids 72-84 phospholipase C zeta 1 Homo sapiens 37-57 17430887-0 2007 Binding of phosphoinositide-specific phospholipase C-zeta (PLC-zeta) to phospholipid membranes: potential role of an unstructured cluster of basic residues. Phospholipids 72-84 phospholipase C zeta 1 Homo sapiens 59-67 16155907-5 2006 The fertilizing sperm, and presumably PLCzeta promote Ca2+ release in eggs via the production of inositol 1,4,5-trisphosphate (IP3), which binds and gates its receptor, the type-1 IP3 receptor, located on the endoplasmic reticulum, the Ca2+ store of the cell. Inositol 1,4,5-Trisphosphate 97-125 phospholipase C zeta 1 Homo sapiens 38-45 17196844-0 2007 Effect of NYD-SP27 down-regulation on ATP-induced Ca2+-dependent pancreatic duct anion secretion in cystic fibrosis cells. Adenosine Triphosphate 38-41 phospholipase C zeta 1 Homo sapiens 10-18 17196844-2 2007 The present study investigated the effect of NYD-SP27 down-regulation on the ATP-stimulated and Ca(2+)-dependent pancreatic anion secretion by CFPAC-1 cell line using short-circuit current (I(SC)) recording. Adenosine Triphosphate 77-80 phospholipase C zeta 1 Homo sapiens 45-53 17196844-10 2007 The present results confirm the previously proposed inhibitory role of NYD-SP27 in the PLC pathway and demonstrate that the suppression of its expression could result in an enhancement of ATP-stimulated Ca(2+) dependent pancreatic anion secretion. Adenosine Triphosphate 188-191 phospholipase C zeta 1 Homo sapiens 71-79 16155907-5 2006 The fertilizing sperm, and presumably PLCzeta promote Ca2+ release in eggs via the production of inositol 1,4,5-trisphosphate (IP3), which binds and gates its receptor, the type-1 IP3 receptor, located on the endoplasmic reticulum, the Ca2+ store of the cell. Inositol 1,4,5-Trisphosphate 127-130 phospholipase C zeta 1 Homo sapiens 38-45 16799264-4 2006 A current strong candidate of the sperm factor is a novel sperm-specific isozyme of phospholipase C (IP(3)-producing enzyme), PLCzeta. Inositol 1,4,5-Trisphosphate 101-106 phospholipase C zeta 1 Homo sapiens 126-133 14697805-6 2003 Suppression of NYD-SP27, by transfection of its antisense into human cystic-fibrosis pancreatic-duct cells, resulted in augmentation of phospholipase-C-coupled calcium-ion release and protein kinase C activity, improvement in the amount of mutated CFTR reaching the plasma membrane, and restoration of cAMP-activated pancreatic anion secretion. Cyclic AMP 302-306 phospholipase C zeta 1 Homo sapiens 15-23 16000311-0 2005 Role of phospholipase C-zeta domains in Ca2+-dependent phosphatidylinositol 4,5-bisphosphate hydrolysis and cytoplasmic Ca2+ oscillations. Phosphatidylinositol 4,5-Diphosphate 55-92 phospholipase C zeta 1 Homo sapiens 8-28 16000311-8 2005 PLCzeta and the closely related PLCdelta1 had similar K(m) values for phosphatidylinositol 4,5-bisphosphate, but PLCzeta was around 100 times more sensitive to Ca2+ than was PLCdelta1. Phosphatidylinositol 4,5-Diphosphate 70-107 phospholipase C zeta 1 Homo sapiens 0-7 16000311-9 2005 Notably, specific phosphatidylinositol 4,5-bisphosphate hydrolysis activity was retained in PLCzeta constructs that had either EF hand domains or the C2 domain removed, or both. Phosphatidylinositol 4,5-Diphosphate 18-55 phospholipase C zeta 1 Homo sapiens 92-99 14697805-7 2003 INTERPRETATION: NYD-SP27 exerts an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C, including CFTR trafficking and function. Calcium 105-112 phospholipase C zeta 1 Homo sapiens 16-24