PMID-sentid	Pub_year	Sent_text	comp_official_name	comp_offset	protein_name	organism	prot_offset
20956596-0	2011	The antibiotic gentamicin inhibits specific protein trafficking functions of the Arf1/2 family of GTPases.	Gentamicins	15-25	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	81-87
25610717-3	2014	Upon activation, Arf1-GTP interacts with effectors such as coat complexes, and is able to recruit different coat complexes to different membrane sites in cells.	Guanosine Triphosphate	22-25	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	17-21
23535599-5	2013	A yeast genetic screen for IpaJ substrates identified ADP-ribosylation factor (ARF)1p and ARF2p, small molecular mass GTPases that regulate cargo transport through the Golgi apparatus.	ipaj	27-31	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	79-85
20956596-7	2011	Further analysis has demonstrated that some conditional arf1 and gea1 alleles make cells hypersensitive to gentamicin under permissive conditions.	Gentamicins	107-117	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	56-60
20214751-4	2010	Here, we have characterized the effect of C-terminal tagging of Arf1 on (i) function in Saccharomyces cerevisiae, (ii) in vitro nucleotide exchange and (iii) interaction with guanine nucleotide exchange factors and GTPase-activating proteins.	Guanine Nucleotides	175-193	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	64-68
20519435-2	2010	Unexpectedly, in Saccharomyces cerevisiae, mutants of the small GTPase Arf1 and various secretory pathway mutants induced a significant increase in PB number, compared with PB induction by starvation or oxidative stress.	pladienolide B	148-150	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	71-75
20519435-2	2010	Unexpectedly, in Saccharomyces cerevisiae, mutants of the small GTPase Arf1 and various secretory pathway mutants induced a significant increase in PB number, compared with PB induction by starvation or oxidative stress.	pladienolide B	173-175	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	71-75
19109418-7	2009	Our findings indicate that the two types of ArfGAP proteins that reside at the Golgi use a different combination of protein-protein and protein-lipid interactions to promote GTP hydrolysis in Arf1-GTP.	Guanosine Triphosphate	174-177	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	192-196
18689681-2	2008	Here, we show that Arf1-GTP induces positive membrane curvature and find that the small GTPase can dimerize dependent on GTP.	Guanosine Triphosphate	24-27	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	19-23
18689681-5	2008	Strikingly, this mutant was not able to deform membranes, suggesting that GTP-induced dimerization of Arf1 is a critical step inducing membrane curvature during the formation of coated vesicles.	Guanosine Triphosphate	74-77	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	102-106
16100119-3	2005	Recruitment of ARF1 and coatomer to peroxisomes was significantly affected both by pretreating the animals with peroxisome proliferators and by ATP and a cytosolic fraction designated the intermediate pool fraction depleted of ARF and coatomer.	Adenosine Triphosphate	144-147	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	15-19
17062999-1	2006	Drs2p, the catalytic subunit of the Cdc50p-Drs2p putative aminophospholipid translocase, has been implicated in conjunction with the Arf1 signaling pathway in the formation of clathrin-coated vesicles (CCVs) from the TGN.	aminophospholipid	58-75	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	133-137
16100119-4	2005	In the presence of ATP, the concentrations of ARF1 and coatomer on peroxisomes were reduced, whereas intermediate pool fraction led to a concentration-dependent decrease in ARF and increase in coatomer.	Adenosine Triphosphate	19-22	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	46-50
16100119-5	2005	Brefeldin A, a fungal toxin that is known to reduce ARF1 binding to Golgi membranes, did not affect ARF1 binding to peroxisomes.	Brefeldin A	0-11	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	52-56
15356266-3	2004	To identify new interactors of Arf1p, we performed an affinity chromatography with GTP- or GDP-bound Arf1p proteins.	Guanosine Triphosphate	83-86	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	31-36
15975906-3	2005	Here, we demonstrate that yeast Gcs1p exhibits GAP activity toward Arl1p and Arf1p in vitro, and Arl1p can interact with Gcs1p in a GTP-dependent manner.	Guanosine Triphosphate	132-135	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	77-82
15930122-5	2005	COPI in these mutants is released from Golgi membranes by brefeldin A, a drug that binds directly to Gea2p and blocks Arf1 activation.	Brefeldin A	58-69	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	118-122
15944734-1	2005	ArfGAP1 promotes GTP hydrolysis in Arf1, a small G protein that interacts with lipid membranes and drives the assembly of the COPI coat in a GTP-dependent manner.	Guanosine Triphosphate	17-20	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	35-39
15944734-1	2005	ArfGAP1 promotes GTP hydrolysis in Arf1, a small G protein that interacts with lipid membranes and drives the assembly of the COPI coat in a GTP-dependent manner.	Guanosine Triphosphate	141-144	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	35-39
15356266-3	2004	To identify new interactors of Arf1p, we performed an affinity chromatography with GTP- or GDP-bound Arf1p proteins.	Guanosine Diphosphate	91-94	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	31-36
15356266-3	2004	To identify new interactors of Arf1p, we performed an affinity chromatography with GTP- or GDP-bound Arf1p proteins.	Guanosine Diphosphate	91-94	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	101-106
12006660-4	2002	All mutations were mapped onto the available crystal structures for Arf1p: Arf1p bound to GDP, to GTP, and complexed with the regulatory proteins ArfGEF and ArfGAP.	Guanosine Triphosphate	98-101	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	68-73
15125774-9	2004	The cytosolic tail of a plant p24 protein is shown to recruit not only coatomer but also ADP ribosylation factor 1 (ARF1), a process which depends on both dilysine and diphenylalanine motifs.	lysyllysine	155-163	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	89-114
15125774-9	2004	The cytosolic tail of a plant p24 protein is shown to recruit not only coatomer but also ADP ribosylation factor 1 (ARF1), a process which depends on both dilysine and diphenylalanine motifs.	lysyllysine	155-163	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	116-120
15125774-9	2004	The cytosolic tail of a plant p24 protein is shown to recruit not only coatomer but also ADP ribosylation factor 1 (ARF1), a process which depends on both dilysine and diphenylalanine motifs.	diphenylalanine	168-183	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	89-114
15125774-9	2004	The cytosolic tail of a plant p24 protein is shown to recruit not only coatomer but also ADP ribosylation factor 1 (ARF1), a process which depends on both dilysine and diphenylalanine motifs.	diphenylalanine	168-183	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	116-120
15125774-10	2004	ARF1 binding increases twofold upon treatment with brefeldin A (BFA) and is completely abolished upon treatment with GTPgammaS, suggesting that ARF1 can only interact with the cytosolic tail of p24 proteins in its GDP-bound form.	Brefeldin A	51-62	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	0-4
15125774-10	2004	ARF1 binding increases twofold upon treatment with brefeldin A (BFA) and is completely abolished upon treatment with GTPgammaS, suggesting that ARF1 can only interact with the cytosolic tail of p24 proteins in its GDP-bound form.	Brefeldin A	64-67	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	0-4
15125774-10	2004	ARF1 binding increases twofold upon treatment with brefeldin A (BFA) and is completely abolished upon treatment with GTPgammaS, suggesting that ARF1 can only interact with the cytosolic tail of p24 proteins in its GDP-bound form.	Guanosine 5'-O-(3-Thiotriphosphate)	117-126	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	0-4
15125774-10	2004	ARF1 binding increases twofold upon treatment with brefeldin A (BFA) and is completely abolished upon treatment with GTPgammaS, suggesting that ARF1 can only interact with the cytosolic tail of p24 proteins in its GDP-bound form.	Guanosine 5'-O-(3-Thiotriphosphate)	117-126	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	144-148
15125774-10	2004	ARF1 binding increases twofold upon treatment with brefeldin A (BFA) and is completely abolished upon treatment with GTPgammaS, suggesting that ARF1 can only interact with the cytosolic tail of p24 proteins in its GDP-bound form.	Guanosine Diphosphate	214-217	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	0-4
15125774-10	2004	ARF1 binding increases twofold upon treatment with brefeldin A (BFA) and is completely abolished upon treatment with GTPgammaS, suggesting that ARF1 can only interact with the cytosolic tail of p24 proteins in its GDP-bound form.	Guanosine Diphosphate	214-217	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	144-148
12627398-1	2003	Previous studies in yeast have revealed the presence of four proteins with a conserved, cysteine-rich, ARF GAP domain that share the ability to suppress the conditional growth defect of the arf1-3 mutant.	Cysteine	88-96	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	190-196
12006660-2	2002	We generated a series of 23 clustered charge-to-alanine mutations in the Arf1 protein of Saccharomyces cerevisiae to determine the portions of this protein important for its function in cells.	Alanine	48-55	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	73-77
12006660-4	2002	All mutations were mapped onto the available crystal structures for Arf1p: Arf1p bound to GDP, to GTP, and complexed with the regulatory proteins ArfGEF and ArfGAP.	Guanosine Diphosphate	90-93	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	68-73
12006660-4	2002	All mutations were mapped onto the available crystal structures for Arf1p: Arf1p bound to GDP, to GTP, and complexed with the regulatory proteins ArfGEF and ArfGAP.	Guanosine Diphosphate	90-93	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	75-80
12006660-4	2002	All mutations were mapped onto the available crystal structures for Arf1p: Arf1p bound to GDP, to GTP, and complexed with the regulatory proteins ArfGEF and ArfGAP.	Guanosine Triphosphate	98-101	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	75-80
12006660-6	2002	In addition, we describe the isolation of a spatially distant intragenic suppressor of a dominant lethal mutation in the guanine nucleotide-binding region of Arf1p.	Guanine Nucleotides	121-139	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	158-163
10930462-9	2000	Consistent with this observation, arf1 mutants exhibit reduced PtdIns(4)P levels.	PtdIns(4)P	63-73	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	34-38
10811810-1	2000	The binding of the coat protein complex, coatomer, to the Golgi is mediated by the small GTPase ADP-ribosylation factor-1 (ARF1), whereas the dissociation of coatomer, requires GTP hydrolysis on ARF1, which depends on a GTPase-activating protein (GAP).	Guanosine Triphosphate	89-92	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	123-127
10811810-3	2000	In this study, we investigated the role of coatomer in GTP hydrolysis on ARF1 both in solution and in a phospholipid environment.	Guanosine Triphosphate	55-58	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	73-77
10811810-4	2000	When GTP hydrolysis was assayed in solution using Delta17-ARF1, coatomer stimulated hydrolysis in the presence of the full-length GAP1 as well as with a Saccharomyces cerevisiae ARF GAP (Gcs1) but had no effect on hydrolysis in the presence of the phosphoinositide dependent GAP, ASAP1.	Guanosine Triphosphate	5-8	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	58-62
10811810-5	2000	Using wild-type myristoylated ARF1 loaded with GTP in the presence of phospholipid vesicles, GAP1 by itself stimulated GTP hydrolysis efficiently, and coatomer had no additional effect.	Guanosine Triphosphate	47-50	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	30-34
10811810-5	2000	Using wild-type myristoylated ARF1 loaded with GTP in the presence of phospholipid vesicles, GAP1 by itself stimulated GTP hydrolysis efficiently, and coatomer had no additional effect.	Phospholipids	70-82	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	30-34
10811810-6	2000	Disruption of the phospholipid vesicles with detergent resulted in reduced GAP1 activity that was stimulated by coatomer, a pattern that resembled Delta17-ARF1 activity.	Phospholipids	18-30	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	154-159
10811810-7	2000	Our findings suggest that in the biological membrane, the proximity between ARF1 and its GAP, which results from mutual binding to membrane phospholipids, may be sufficient for stimulation of ARF1 GTPase activity.	Phospholipids	140-153	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	76-80
10811810-7	2000	Our findings suggest that in the biological membrane, the proximity between ARF1 and its GAP, which results from mutual binding to membrane phospholipids, may be sufficient for stimulation of ARF1 GTPase activity.	Phospholipids	140-153	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	192-196
11294905-1	2001	The activation of the small ras-like GTPase Arf1p requires the action of guanine nucleotide exchange factors.	Guanine Nucleotides	73-91	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	44-49
11294905-2	2001	Four Arf1p guanine nucleotide exchange factors have been identified in yeast: Sec7p, Syt1p, Gea1p, and its homologue Gea2p.	Guanine Nucleotides	11-29	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	5-10
9693364-2	1998	The addition of the c-myc epitope at the C terminus of Arf1 resulted in a mutant (arf1-myc arf2) that supported vegetative growth and rescued cells from supersensitivity to fluoride, but homozygous diploids failed to sporulate.	Fluorides	173-181	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	55-59
10747089-5	2000	Treatment with brefeldin A or overexpression of dominant-negative ADP ribosylation factor 1 (ARF1) caused dissociation of GGAs from membranes.	Brefeldin A	15-26	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	93-97
9693364-2	1998	The addition of the c-myc epitope at the C terminus of Arf1 resulted in a mutant (arf1-myc arf2) that supported vegetative growth and rescued cells from supersensitivity to fluoride, but homozygous diploids failed to sporulate.	Fluorides	173-181	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	82-95
7879913-15	1995	S. cerevisiae arf1 mutants presented a phenotype similar to that of BFA-treated S. cerevisiae erg6.	Brefeldin A	68-71	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	14-18
9539714-7	1998	The His6-tagged Sec7 domain from ySec7p (rySec7d) synthesized in Escherichia coli enhanced binding of guanosine 5"-[gamma-[35S]thio]triphosphate by recombinant yeast ARF1 (ryARF1) and ryARF2 but not by ryARF3.	guanosine 5"-[gamma-[35s]thio]triphosphate	102-144	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	166-170
9442017-4	1998	Further, the Sec7 domain of GRP1 is found to catalyze guanine nucleotide exchange of ARF1 and -5 but not ARF6.	Guanine Nucleotides	54-72	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	85-96
9442017-7	1998	Taken together, these data are consistent with the hypothesis that selective recruitment of GRP1 to PtdIns(3,4,5)P3 in membranes activates ARF1 and -5, known regulators of intracellular membrane trafficking.	phosphatidylinositol 3,4,5-triphosphate	100-115	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	139-150
8816753-8	1996	Moreover, cells with decreased levels of Arf1 or Arf2 protein, and thus with decreased levels of GTP-Arf, are markedly inhibited for growth by increased GCS1 gene dosage, presumably because increased levels of Gcs1 GAP activity further decrease GTP-Arf levels.	Guanosine Triphosphate	245-248	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	41-45
8440712-3	1993	Incorporation of exogenous tritiated myristate into Arf1p and bacterial phospholipid biosynthetic pathways was analyzed.	tritiated myristate	27-46	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	52-57
8440712-10	1993	It can fully "restore" N-myristoylation of Arf1p.	Nitrogen	23-24	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	43-48
2123295-10	1990	Disruption of ARF1 causes slow growth, cold sensitivity, and sensitivity to normally sublethal concentrations of fluoride ion in the medium.	Fluorides	113-121	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	14-18
2123295-14	1990	Among revertants of the fluoride sensitivity of an arf1 null mutation were ARF1-ARF2 fusion genes created by a gene conversion event in which the deleted ARF1 sequences were repaired by recombination with ARF2.	Fluorides	24-32	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	51-55
2123295-14	1990	Among revertants of the fluoride sensitivity of an arf1 null mutation were ARF1-ARF2 fusion genes created by a gene conversion event in which the deleted ARF1 sequences were repaired by recombination with ARF2.	Fluorides	24-32	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	75-79
2123295-14	1990	Among revertants of the fluoride sensitivity of an arf1 null mutation were ARF1-ARF2 fusion genes created by a gene conversion event in which the deleted ARF1 sequences were repaired by recombination with ARF2.	Fluorides	24-32	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	154-158
33401608-2	2021	Two related types of guanine nucleotide exchange factors (GEFs) activate Arf1 at different Golgi sites.	Guanine Nucleotides	21-39	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	73-77
2105501-5	1990	Genetic experiments revealed interactions between ARF1 and other genes known to be involved in the secretory pathway, including YPT1, which encodes a different GTP-binding protein.	Guanosine Triphosphate	160-163	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	50-54
31331965-7	2019	We demonstrate that Glo3/ArfGAP2/3 specifically triggers Arf1 GTP hydrolysis impinging on the stability of the COPI coat.	Guanosine Triphosphate	62-65	Arf family GTPase ARF1	Saccharomyces cerevisiae S288C	57-61