PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 34553070-3 2021 Different enantiomers of amlodipine did not influence lipid profiles and serum level of eNOS in the rabbit atherosclerosis model but decreased ET-1 expression to some extent. Amlodipine 25-35 endothelin-1 Oryctolagus cuniculus 143-147 34553070-8 2021 Both S-amlodipine and R-amlodipine may protect the endothelium by reducing the serum ET-1 level, downregulating iNOS expression. Amlodipine 5-17 endothelin-1 Oryctolagus cuniculus 85-89 34553070-8 2021 Both S-amlodipine and R-amlodipine may protect the endothelium by reducing the serum ET-1 level, downregulating iNOS expression. (R)-Amlodipine 22-34 endothelin-1 Oryctolagus cuniculus 85-89 2679558-0 1989 Endothelin-1 increases intracellular calcium mobilization but not calcium uptake in rabbit vascular smooth muscle cells. Calcium 37-44 endothelin-1 Oryctolagus cuniculus 0-12 2679558-1 1989 Conflicting evidence has been reported regarding the role of endothelin-1, a potent vasconstrictor peptide, in stimulating extracellular calcium influx in rabbit vascular smooth muscle. Calcium 137-144 endothelin-1 Oryctolagus cuniculus 61-73 2679558-3 1989 In calcium containing buffer, endothelin-1 induced a concentration-dependent 45Ca2+ efflux response over the range of 10 pM to 100 nM with an EC50 of approximately 60 pM. Calcium 3-10 endothelin-1 Oryctolagus cuniculus 30-42 2476324-5 1989 Contractions induced by ET-1 were not affected by the kininase II inhibitor captopril in the rabbit jugular veins but were potentiated by methylene blue in both veins of the rabbit. Methylene Blue 138-152 endothelin-1 Oryctolagus cuniculus 24-28 2473289-0 1989 Endothelin-1 releases prostacyclin and inhibits ex vivo platelet aggregation in the anesthetized rabbit. Epoprostenol 22-34 endothelin-1 Oryctolagus cuniculus 0-12 2473289-3 1989 ET-1 inhibited ADP-induced platelet aggregation by 83 +/- 9% within 5 min (p less than 0.01). Adenosine Diphosphate 15-18 endothelin-1 Oryctolagus cuniculus 0-4 2473289-4 1989 This ET-1-induced inhibition of platelet aggregation lasted for 30 min and was associated with a significant (five- to sixfold) increase in platelet cAMP. Cyclic AMP 149-153 endothelin-1 Oryctolagus cuniculus 5-9 2473289-6 1989 Indomethacin (5 mg/kg i.v., 20 min prior to ET-1) abolished the ET-1-induced inhibition of ex vivo platelet aggregation as well as the corresponding augmentation of platelet cAMP. Indomethacin 0-12 endothelin-1 Oryctolagus cuniculus 44-48 2473289-6 1989 Indomethacin (5 mg/kg i.v., 20 min prior to ET-1) abolished the ET-1-induced inhibition of ex vivo platelet aggregation as well as the corresponding augmentation of platelet cAMP. Indomethacin 0-12 endothelin-1 Oryctolagus cuniculus 64-68 2473282-4 1989 Ring preparations of canine coronary artery and rabbit thoracic aorta showed vasoconstrictive responses to ET-1 (10(-10) M and over) with an EC50 of 3-10 x 10(-9) M. Nifedipine 10(-5) M did not reverse these effects completely. Nifedipine 166-176 endothelin-1 Oryctolagus cuniculus 107-111 2473289-6 1989 Indomethacin (5 mg/kg i.v., 20 min prior to ET-1) abolished the ET-1-induced inhibition of ex vivo platelet aggregation as well as the corresponding augmentation of platelet cAMP. Cyclic AMP 174-178 endothelin-1 Oryctolagus cuniculus 64-68 2473289-7 1989 Thus, ET-1 inhibits platelet function in vivo due to the release into the circulation of antiplatelet cyclo-oxygenase products, such as prostacyclin (or PGD2). Epoprostenol 136-148 endothelin-1 Oryctolagus cuniculus 6-10 2473289-7 1989 Thus, ET-1 inhibits platelet function in vivo due to the release into the circulation of antiplatelet cyclo-oxygenase products, such as prostacyclin (or PGD2). Prostaglandin D2 153-157 endothelin-1 Oryctolagus cuniculus 6-10 30656600-10 2021 Furthermore, compared with the MI group, the plasma levels of TXA2, ET-1 and vWF contents signififi cantly decreased in the MI+SSYX group, and the ET-1 mRNA expression levels of myocardium in the border zone significantly decreased, and the VEGF, PGI2 and eNOS mRNA expression levels signififi cantly increased (all P<0.05). Epoprostenol 247-251 endothelin-1 Oryctolagus cuniculus 147-151 2473330-2 1989 We investigated the inhibition of ET-1-induced contractions on isolated porcine coronary artery and rabbit aorta by calcium antagonists of the 1,4-dihydropyridine type. Calcium 116-123 endothelin-1 Oryctolagus cuniculus 34-38 2473330-2 1989 We investigated the inhibition of ET-1-induced contractions on isolated porcine coronary artery and rabbit aorta by calcium antagonists of the 1,4-dihydropyridine type. 1,4-dihydropyridine 143-162 endothelin-1 Oryctolagus cuniculus 34-38 2473330-4 1989 The calcium antagonists nitrendipine and nicardipine partially antagonize the vasoconstrictive effects of ET-1 in a dose-dependent manner, but this antagonism is only functional and noncompetitive. Calcium 4-11 endothelin-1 Oryctolagus cuniculus 106-110 2473330-4 1989 The calcium antagonists nitrendipine and nicardipine partially antagonize the vasoconstrictive effects of ET-1 in a dose-dependent manner, but this antagonism is only functional and noncompetitive. Nitrendipine 24-36 endothelin-1 Oryctolagus cuniculus 106-110 2473330-4 1989 The calcium antagonists nitrendipine and nicardipine partially antagonize the vasoconstrictive effects of ET-1 in a dose-dependent manner, but this antagonism is only functional and noncompetitive. Nicardipine 41-52 endothelin-1 Oryctolagus cuniculus 106-110 2473330-8 1989 Again here, the concentration-response curve of ET-1 is depressed by increasing concentrations of nitrendipine, but only functionally, noncompetitively. Nitrendipine 98-110 endothelin-1 Oryctolagus cuniculus 48-52 2473335-6 1989 Release of EDRF from the rabbit aorta in response to ET-1 but not to other agonists (acetylcholine, substance P, or adenosine diphosphate) was potentiated by infusion of potassium chloride (3 mM). Potassium Chloride 170-188 endothelin-1 Oryctolagus cuniculus 53-57 2473336-0 1989 Endothelin-1 releases eicosanoids from rabbit isolated perfused kidney and spleen. Eicosanoids 22-33 endothelin-1 Oryctolagus cuniculus 0-12 2473336-1 1989 This study analyzes the effects of porcine endothelin-1 (ET-1) on perfusion pressure and eicosanoid release from rabbit isolated spleen and kidney. Eicosanoids 89-99 endothelin-1 Oryctolagus cuniculus 57-61 2473336-5 1989 In the spleen, ET-1 stimulated the release of PGE2 mainly and, to a lesser extent, of PGI2 and TXA2. Dinoprostone 46-50 endothelin-1 Oryctolagus cuniculus 15-19 2473336-5 1989 In the spleen, ET-1 stimulated the release of PGE2 mainly and, to a lesser extent, of PGI2 and TXA2. Epoprostenol 86-90 endothelin-1 Oryctolagus cuniculus 15-19 2473336-6 1989 In the kidney, ET-1 stimulated a greater release of PGI2 than PGE2, but not TXA2. Epoprostenol 52-56 endothelin-1 Oryctolagus cuniculus 15-19 2473336-7 1989 These results demonstrate that in the isolated rabbit spleen and kidney, ET-1 is a potent vasoconstrictor and triggers the generation of vasodilator eicosanoids that limit the pressor effects of the peptide. Eicosanoids 149-160 endothelin-1 Oryctolagus cuniculus 73-77 32098974-14 2020 The blood oxygen saturation was negatively correlated with the levels of ET-1 and Ang II. Oxygen 10-16 endothelin-1 Oryctolagus cuniculus 73-88 32098974-15 2020 OSAHS-induced elevated levels of ET-1 and Ang II may be attributed to myocardial structural abnormalities and dysfunction. osahs 0-5 endothelin-1 Oryctolagus cuniculus 33-48 30244111-11 2018 Compared with SAH group, plasma ET-1 levels in BQ123 group significantly increased on the earlier (1st and 4th days) but not later days (between the 7th and 14th days). cyclo(Trp-Asp-Pro-Val-Leu) 47-52 endothelin-1 Oryctolagus cuniculus 32-36 31118045-6 2019 Both SNP and DLZ decreased mean pulmonary arterial pressure, reversed shock status, downregulated the expression of TH, NPY and ET-1, and increased NO levels in PE and non-PE regions. DLZ 13-16 endothelin-1 Oryctolagus cuniculus 128-132 29565509-8 2018 The NO, O2- and ET-1 levels in both the AS and AS + nitrate ester tolerance groups were significantly decreased, but SOD and MDA were significantly increased (p < 0.05). as + nitrate ester 48-66 endothelin-1 Oryctolagus cuniculus 17-21 30086070-8 2018 The iNOS and ET-1 inhibitors, aminoguanidine (20 mg/kg) and PD-142893 (0.02 mg/kg), respectively, induced significant improvements in vascular reactivity and organ perfusion and stabilized the hemodynamic parameters in rabbits subjected to endotoxic shock. pimagedine 30-44 endothelin-1 Oryctolagus cuniculus 13-17 30086070-8 2018 The iNOS and ET-1 inhibitors, aminoguanidine (20 mg/kg) and PD-142893 (0.02 mg/kg), respectively, induced significant improvements in vascular reactivity and organ perfusion and stabilized the hemodynamic parameters in rabbits subjected to endotoxic shock. PD 142893 60-69 endothelin-1 Oryctolagus cuniculus 13-17 30110059-5 2018 A significant correlation was observed between the peak plasma CTnI concentration and peak TXB2, 6-keto prostaglandin F1alpha and ET-1 concentrations in the model and NOI groups. ctni 63-67 endothelin-1 Oryctolagus cuniculus 130-134 29886166-0 2018 Attenuation of the degenerative effects of endothelin-1 on cartilaginous end plate cells by the endothelin receptor antagonist BQ-123 via the Wnt/beta-catenin signaling pathway. cyclo(Trp-Asp-Pro-Val-Leu) 127-133 endothelin-1 Oryctolagus cuniculus 43-55 29886166-4 2018 PURPOSE: The purpose of the study was to evaluate the influence of the endothelin-A receptor antagonist, BQ-123, on ET-1-induced effects on cartilaginous end plate cells (CECs) associated with CEP degeneration via the Wnt/beta-catenin signaling pathway. cyclo(Trp-Asp-Pro-Val-Leu) 105-111 endothelin-1 Oryctolagus cuniculus 116-120 29886166-15 2018 In addition, ET-1 stimulation increased the expression of beta-catenin, cyclin D1, and Dvl1 in the Wnt/beta-catenin signaling pathway of CECs from degenerated discs and reduced the expression of GSK-3beta, whereas BQ-123 had the opposite effect. cyclo(Trp-Asp-Pro-Val-Leu) 214-220 endothelin-1 Oryctolagus cuniculus 13-17 22683635-8 2012 When ET-1 was applied following ACh, the ET-1 activated response was greatly attenuated, demonstrating that ACh could desensitize the response to ET-1. Acetylcholine 32-35 endothelin-1 Oryctolagus cuniculus 5-9 29257200-10 2018 Administration of atorvastatin may significantly downregulate the expression levels of ET-1, vWF and TM (all P<0.01) vs. sham and SAH groups. Atorvastatin 18-30 endothelin-1 Oryctolagus cuniculus 87-91 27391329-3 2016 Inhibition of NF-kappaB with pyrrolidine dithiocarbamate and small interfering RNA decreased the expression of ET-1. pyrrolidine dithiocarbamic acid 29-56 endothelin-1 Oryctolagus cuniculus 111-115 27391329-5 2016 The supernatant obtained from cerebrovascular muscle cells stimulated by OxyHb produced contractions in arterial rings and was blocked by the ET-1 receptor antagonist (BQ-123). cyclo(Trp-Asp-Pro-Val-Leu) 168-174 endothelin-1 Oryctolagus cuniculus 142-146 23816794-11 2013 BQ123 reduced both ET-1-induced [Ca(2+)]i increase and cell death for myocytes subjected to stimulated I/R. cyclo(Trp-Asp-Pro-Val-Leu) 0-5 endothelin-1 Oryctolagus cuniculus 19-23 23816794-12 2013 CONCLUSION: We propose that components of reperfusion injury involve ET-1 release which stimulates calcium overload and apoptosis. Calcium 99-106 endothelin-1 Oryctolagus cuniculus 69-73 29207193-0 2018 All-trans retinoic acid reduces endothelin-1 expression and increases endothelial nitric oxide synthase phosphorylation in rabbits with atherosclerosis. Tretinoin 0-23 endothelin-1 Oryctolagus cuniculus 32-44 29207193-8 2018 Western blotting demonstrated significantly decreased ET-1 expression levels in the arterial tissue of rabbits in the ATRA group compared with the high fat group, together with increased p-eNOS level (P<0.05), however, no difference was observed in the expression of eNOS (P>0.05). Tretinoin 118-122 endothelin-1 Oryctolagus cuniculus 54-58 29207193-10 2018 Therefore, the present study demonstrated that ATRA may regulate the grade of AS by the reduction of ET-1 secretion and increased NO formation via increased phosphorylation of eNOS. Tretinoin 47-51 endothelin-1 Oryctolagus cuniculus 101-105 27044361-10 2016 In the celecoxib group compared to the SAH group, expression of COX-2, ET-1, and ETAR were statistically significantly decreased, and eNOS expression was significantly increased. Celecoxib 7-16 endothelin-1 Oryctolagus cuniculus 71-75 27941335-0 2016 Mechanisms Underlying Endothelin-1 Level Elevations Caused by Excessive Fluoride Exposure. Fluorides 72-80 endothelin-1 Oryctolagus cuniculus 22-34 27941335-1 2016 OBJECTIVE: To explore the mechanisms underlying endothelin-1 (ET-1) elevations induced by excessive fluoride exposure. Fluorides 100-108 endothelin-1 Oryctolagus cuniculus 48-60 27941335-1 2016 OBJECTIVE: To explore the mechanisms underlying endothelin-1 (ET-1) elevations induced by excessive fluoride exposure. Fluorides 100-108 endothelin-1 Oryctolagus cuniculus 62-66 27941335-9 2016 After U0126 was added, ECE-1 expression and ET-1 levels decreased significantly. U 0126 6-11 endothelin-1 Oryctolagus cuniculus 44-48 27941335-10 2016 CONCLUSION: Excessive fluoride exposure leads to characteristic endothelial damage (vacuoles), thoracic aorta hardening, and plasma ET-1 level elevations in rabbits. Fluorides 22-30 endothelin-1 Oryctolagus cuniculus 132-136 27941335-11 2016 In addition, the ROS-RAS-MEK1/2-pERK1/2/ERK1/2 pathway plays a crucial-and at least partial-role in ET-1 over-expression, which is promoted by excessive fluoride exposure. Reactive Oxygen Species 17-20 endothelin-1 Oryctolagus cuniculus 100-104 27941335-11 2016 In addition, the ROS-RAS-MEK1/2-pERK1/2/ERK1/2 pathway plays a crucial-and at least partial-role in ET-1 over-expression, which is promoted by excessive fluoride exposure. Fluorides 153-161 endothelin-1 Oryctolagus cuniculus 100-104 26807018-0 2016 cAMP induction by ouabain promotes endothelin-1 secretion via MAPK/ERK signaling in beating rabbit atria. Cyclic AMP 0-4 endothelin-1 Oryctolagus cuniculus 35-47 26807018-0 2016 cAMP induction by ouabain promotes endothelin-1 secretion via MAPK/ERK signaling in beating rabbit atria. Ouabain 18-25 endothelin-1 Oryctolagus cuniculus 35-47 26807018-3 2016 The aim of the present study was to examine the effect of ouabain on the regulation of atrial cAMP production and its roles in atrial endothelin-1 (ET-1) secretion in isolated perfused beating rabbit atria. Ouabain 58-65 endothelin-1 Oryctolagus cuniculus 134-146 26807018-3 2016 The aim of the present study was to examine the effect of ouabain on the regulation of atrial cAMP production and its roles in atrial endothelin-1 (ET-1) secretion in isolated perfused beating rabbit atria. Ouabain 58-65 endothelin-1 Oryctolagus cuniculus 148-152 26807018-7 2016 Ouabain and 8-Bromo-cAMP (0.5 micromol/L) markedly increased atrial ET-1 secretion, which was blocked by H-89 and by PD98059 (30 micromol/L), an inhibitor of extracellular-signal-regulated kinase (ERK) without changing ouabain-induced atrial dynamics. Ouabain 0-7 endothelin-1 Oryctolagus cuniculus 68-72 26807018-7 2016 Ouabain and 8-Bromo-cAMP (0.5 micromol/L) markedly increased atrial ET-1 secretion, which was blocked by H-89 and by PD98059 (30 micromol/L), an inhibitor of extracellular-signal-regulated kinase (ERK) without changing ouabain-induced atrial dynamics. 8-Bromo Cyclic Adenosine Monophosphate 12-24 endothelin-1 Oryctolagus cuniculus 68-72 26807018-7 2016 Ouabain and 8-Bromo-cAMP (0.5 micromol/L) markedly increased atrial ET-1 secretion, which was blocked by H-89 and by PD98059 (30 micromol/L), an inhibitor of extracellular-signal-regulated kinase (ERK) without changing ouabain-induced atrial dynamics. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 117-124 endothelin-1 Oryctolagus cuniculus 68-72 26807018-7 2016 Ouabain and 8-Bromo-cAMP (0.5 micromol/L) markedly increased atrial ET-1 secretion, which was blocked by H-89 and by PD98059 (30 micromol/L), an inhibitor of extracellular-signal-regulated kinase (ERK) without changing ouabain-induced atrial dynamics. Ouabain 219-226 endothelin-1 Oryctolagus cuniculus 68-72 25399298-2 2015 Infusion of ET-1 into isolated rabbit lung has been shown to cause pulmonary vasoconstriction with the involvement of arachidonic acid metabolites. Arachidonic Acid 118-134 endothelin-1 Oryctolagus cuniculus 12-16 24735960-9 2014 KEY FINDINGS: Here, we show that both circulatory and pulmonary ET-1 levels increased in models with lung injury induced by saline lavage compared to healthy control group. Sodium Chloride 124-130 endothelin-1 Oryctolagus cuniculus 64-68 24735960-12 2014 SIGNIFICANCE: We conclude that in a saline lavage-induced lung injury model, both circulatory and pulmonary ET-1 levels increased. Sodium Chloride 36-42 endothelin-1 Oryctolagus cuniculus 108-112 23816794-2 2013 We tested that endogenous ET-1 released during acute myocardial infarction might mediate ischemia/reperfusion (I/R) injury by stimulating increased intracellular calcium concentration, [Ca(2+)]i, and apoptosis. Calcium 162-169 endothelin-1 Oryctolagus cuniculus 26-30 23816794-6 2013 Animals were treated with ET-1 receptor antagonist BQ123. cyclo(Trp-Asp-Pro-Val-Leu) 51-56 endothelin-1 Oryctolagus cuniculus 26-30 22683635-8 2012 When ET-1 was applied following ACh, the ET-1 activated response was greatly attenuated, demonstrating that ACh could desensitize the response to ET-1. Acetylcholine 32-35 endothelin-1 Oryctolagus cuniculus 41-45 22683635-8 2012 When ET-1 was applied following ACh, the ET-1 activated response was greatly attenuated, demonstrating that ACh could desensitize the response to ET-1. Acetylcholine 32-35 endothelin-1 Oryctolagus cuniculus 41-45 22683635-8 2012 When ET-1 was applied following ACh, the ET-1 activated response was greatly attenuated, demonstrating that ACh could desensitize the response to ET-1. Acetylcholine 108-111 endothelin-1 Oryctolagus cuniculus 5-9 22683635-8 2012 When ET-1 was applied following ACh, the ET-1 activated response was greatly attenuated, demonstrating that ACh could desensitize the response to ET-1. Acetylcholine 108-111 endothelin-1 Oryctolagus cuniculus 41-45 22683635-8 2012 When ET-1 was applied following ACh, the ET-1 activated response was greatly attenuated, demonstrating that ACh could desensitize the response to ET-1. Acetylcholine 108-111 endothelin-1 Oryctolagus cuniculus 41-45 22261087-12 2012 Induction of the endogenous HO-1/CO system by hemin can prevent atherosclerosis though increasing CO levels, regulating eNOS activity, NF-kappaB activity, TNF-alpha levels, and ET-1 levels in rabbits. Carbon Monoxide 33-35 endothelin-1 Oryctolagus cuniculus 177-181 22521291-7 2012 Ouabain-increased atrial ET-1 release was blocked by PD98059 (30.0 mumol/L), an inhibitor of mitogen-activated protein kinase (MAPK). 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 53-60 endothelin-1 Oryctolagus cuniculus 25-29 22521291-11 2012 The effects of ET-1 and ouabain on ANP secretion were completely blocked by BQ788 (0.3 mumol/L), an inhibitor of ET-1 type B (ET(B)) receptors, but not by BQ123 (0.3 muM), an inhibitor of ET-1 type A receptors. BQ 788 76-81 endothelin-1 Oryctolagus cuniculus 15-19 22521291-11 2012 The effects of ET-1 and ouabain on ANP secretion were completely blocked by BQ788 (0.3 mumol/L), an inhibitor of ET-1 type B (ET(B)) receptors, but not by BQ123 (0.3 muM), an inhibitor of ET-1 type A receptors. BQ 788 76-81 endothelin-1 Oryctolagus cuniculus 113-117 22521291-13 2012 SIGNIFICANCE: Ouabain significantly stimulated atrial ANP secretion via an ET-1-ET(B) receptor-mediated pathway involving MAPK signaling pathway activation and prostaglandin formation. Prostaglandins 160-173 endothelin-1 Oryctolagus cuniculus 75-79 22257787-8 2012 Both NCX 434 and TA reversed ET-1 induced decrease in electroretinography amplitude to similar extents. ncx 5-8 endothelin-1 Oryctolagus cuniculus 29-33 22257787-9 2012 NCX 434 attenuated ET-1 induced oxidative stress markers and nitrotyrosine in retinal tissue, and interleukin-6 and tumour necrosis factor-alpha in aqueous humour more effectively than TA. 3-nitrotyrosine 61-74 endothelin-1 Oryctolagus cuniculus 19-23 25737711-3 2012 Results revealed that propofol inhibited serum interleukin-8, endothelin-1 and malondialdehyde increases and promoted plasma superoxide dismutase activity after cerebral ischemia/reperfusion. Propofol 22-30 endothelin-1 Oryctolagus cuniculus 62-74 20868682-1 2010 We investigated the effects of prostaglandin F(2alpha) (PGF(2alpha)) analogues on the endothelin-1 (ET-1)-induced impairment of optic nerve head (ONH) blood flow and on ET-1-induced contraction in isolated ciliary artery segments. Prostaglandins F 31-46 endothelin-1 Oryctolagus cuniculus 86-98 20541920-0 2011 Amlodipine treatment decreases plasma and carotid artery tissue levels of endothelin-1 in atherosclerotic rabbits. Amlodipine 0-10 endothelin-1 Oryctolagus cuniculus 74-86 20541920-3 2011 In this study, we have evaluated the effects of amlodipine treatment and/or high-cholesterol diet on blood and carotid artery tissue concentration of ET-1 in the atherosclerotic rabbits. Amlodipine 48-58 endothelin-1 Oryctolagus cuniculus 150-154 20541920-9 2011 Amlodipine treatment significantly reduced ET-1 level in NA and HA rabbits (p<0.01). Amlodipine 0-10 endothelin-1 Oryctolagus cuniculus 43-47 20541920-12 2011 Amlodipine treatment reduced levels of total cholesterol, LDL and TG as well as plasma and carotid tissue levels of ET-1 in high lipid situation. Amlodipine 0-10 endothelin-1 Oryctolagus cuniculus 116-120 20541920-13 2011 We suggest that amlodipine treatment by reducing the ET-1 may contribute to reducing the progression of atherosclerotic disease. Amlodipine 16-26 endothelin-1 Oryctolagus cuniculus 53-57 32092834-12 2012 Induction of the endogenous HO-1/CO system by hemin can prevent atherosclerosis though increasing CO levels, regulating eNOS activity, NF-kappaB activity, TNF-alpha levels, and ET-1 levels in rabbits. Carbon Monoxide 33-35 endothelin-1 Oryctolagus cuniculus 177-181 22912821-7 2012 Using specific signaling antagonists, inhibition of NCX, CaMKII, MAPKK, and IP3 could attenuate the effect of ET-1 on increased developed force. Inositol 1,4,5-Trisphosphate 76-79 endothelin-1 Oryctolagus cuniculus 110-114 21652209-1 2011 A series of novel 2-[(4,6-dimethylpyrimidin-2-yl)oxy]-3,3-diphenyl butyric acid derivatives were synthesized and evaluated for their antagonistic activity for endothelin-1-induced contraction in rabbit aorta. 2-[(4,6-dimethylpyrimidin-2-yl)oxy]-3,3-diphenyl butyric acid 18-79 endothelin-1 Oryctolagus cuniculus 159-171 21914349-13 2011 Compared with the Ch group, rabbits of the Hm group demonstrated a marked reduction of aorta ET-1 expression, whereas Zn group had a significantly higher ET-1 expression. medrysone 43-45 endothelin-1 Oryctolagus cuniculus 93-97 21914349-13 2011 Compared with the Ch group, rabbits of the Hm group demonstrated a marked reduction of aorta ET-1 expression, whereas Zn group had a significantly higher ET-1 expression. Zinc 118-120 endothelin-1 Oryctolagus cuniculus 154-158 20868682-1 2010 We investigated the effects of prostaglandin F(2alpha) (PGF(2alpha)) analogues on the endothelin-1 (ET-1)-induced impairment of optic nerve head (ONH) blood flow and on ET-1-induced contraction in isolated ciliary artery segments. Prostaglandins F 56-59 endothelin-1 Oryctolagus cuniculus 86-98 20868682-6 2010 The ET-1-induced decrease was almost completely prevented by tafluprost and significantly inhibited by the other three analogues. tafluprost 61-71 endothelin-1 Oryctolagus cuniculus 4-8 20868682-8 2010 In vitro, the effects of PGF(2alpha) analogues on ET-1-induced contractions in male rabbit ciliary arteries were evaluated using an isometric tension recording system. Prostaglandins F 25-28 endothelin-1 Oryctolagus cuniculus 50-54 20868682-9 2010 Tafluprost, latanoprost, travoprost, and 15-OH tafluprost concentration-dependently relaxed the 10 nM ET-1-induced ciliary artery contraction. tafluprost 0-10 endothelin-1 Oryctolagus cuniculus 102-106 20868682-9 2010 Tafluprost, latanoprost, travoprost, and 15-OH tafluprost concentration-dependently relaxed the 10 nM ET-1-induced ciliary artery contraction. Latanoprost 12-23 endothelin-1 Oryctolagus cuniculus 102-106 20868682-9 2010 Tafluprost, latanoprost, travoprost, and 15-OH tafluprost concentration-dependently relaxed the 10 nM ET-1-induced ciliary artery contraction. Travoprost 25-35 endothelin-1 Oryctolagus cuniculus 102-106 20868682-9 2010 Tafluprost, latanoprost, travoprost, and 15-OH tafluprost concentration-dependently relaxed the 10 nM ET-1-induced ciliary artery contraction. 15-oh tafluprost 41-57 endothelin-1 Oryctolagus cuniculus 102-106 20306886-12 2010 ET-1 levels were decreased by valsartan treatment compared to levels in the HC group. Valsartan 30-39 endothelin-1 Oryctolagus cuniculus 0-4 20852052-6 2010 In rabbit atrial myocytes, an overnight stimulation with endothelin-1, angiotensin II, and phenylephrine induced nuclear accumulation of NFATc1 that was sensitive to calcineurin inhibitors (cyclosporin A or inhibitor of NFAT-calcineurin association-6) and prevented by the IP(3) receptor inhibitor 2-aminoethoxydiphenyl borate. Cyclosporine 190-203 endothelin-1 Oryctolagus cuniculus 57-69 20852052-6 2010 In rabbit atrial myocytes, an overnight stimulation with endothelin-1, angiotensin II, and phenylephrine induced nuclear accumulation of NFATc1 that was sensitive to calcineurin inhibitors (cyclosporin A or inhibitor of NFAT-calcineurin association-6) and prevented by the IP(3) receptor inhibitor 2-aminoethoxydiphenyl borate. nfat-calcineurin association-6 220-250 endothelin-1 Oryctolagus cuniculus 57-69 20852052-6 2010 In rabbit atrial myocytes, an overnight stimulation with endothelin-1, angiotensin II, and phenylephrine induced nuclear accumulation of NFATc1 that was sensitive to calcineurin inhibitors (cyclosporin A or inhibitor of NFAT-calcineurin association-6) and prevented by the IP(3) receptor inhibitor 2-aminoethoxydiphenyl borate. 2-aminoethoxydiphenyl borate 298-326 endothelin-1 Oryctolagus cuniculus 57-69 20444986-10 2010 ET-1-induced ROS production was inhibited by blocking either NOX or mitochondrial complex I, whereas complex III-induced ROS production was insensitive to NOX blockade. Reactive Oxygen Species 13-16 endothelin-1 Oryctolagus cuniculus 0-4 20444986-11 2010 CONCLUSION: ET-1-ET(A) signalling activated I(Cl,swell) via EGFR kinase, PI-3K, and NOX ROS production, which triggered mitochondrial ROS production. Reactive Oxygen Species 88-91 endothelin-1 Oryctolagus cuniculus 12-16 20444986-11 2010 CONCLUSION: ET-1-ET(A) signalling activated I(Cl,swell) via EGFR kinase, PI-3K, and NOX ROS production, which triggered mitochondrial ROS production. Reactive Oxygen Species 134-137 endothelin-1 Oryctolagus cuniculus 12-16 20444986-13 2010 These data suggest that ET-1-induced ROS-dependent I(Cl,swell) is likely to participate in multiple physiological and pathophysiological processes. Reactive Oxygen Species 37-40 endothelin-1 Oryctolagus cuniculus 24-28 20234381-9 2010 YM254890 also inhibited the endothelin-1- and phenylephrine-induced sustained contraction. YM-254890 0-8 endothelin-1 Oryctolagus cuniculus 28-40 19626279-1 2010 The purpose of this research is to investigate the expression of matrix metalloproteinase-9 (MMP-9) in retinal ganglion cells (RGC) and the impact of topically applied brimonidine tartrate 0.2% (BMD) on this expression in an endothelin-1 (ET-1)-induced chronic optic nerve (ON) ischemia model of rabbit. Brimonidine Tartrate 168-188 endothelin-1 Oryctolagus cuniculus 225-237 19626279-1 2010 The purpose of this research is to investigate the expression of matrix metalloproteinase-9 (MMP-9) in retinal ganglion cells (RGC) and the impact of topically applied brimonidine tartrate 0.2% (BMD) on this expression in an endothelin-1 (ET-1)-induced chronic optic nerve (ON) ischemia model of rabbit. Brimonidine Tartrate 168-188 endothelin-1 Oryctolagus cuniculus 239-243 20306886-14 2010 CONCLUSION: Our data demonstrates that HC diet-induced atherosclerotic lesions in rabbit pulmonary arteries can be ameliorated by treatment with valsartan, possibly through a NO and ET-1-dependent mechanism. Valsartan 145-154 endothelin-1 Oryctolagus cuniculus 182-186 19770190-0 2009 Activation of native TRPC1/C5/C6 channels by endothelin-1 is mediated by both PIP3 and PIP2 in rabbit coronary artery myocytes. PIP3 78-82 endothelin-1 Oryctolagus cuniculus 45-57 19921236-3 2010 (2) Can intraarterial amlodipine counteract ET-1 induced vasoconstriction? Amlodipine 22-32 endothelin-1 Oryctolagus cuniculus 44-48 19921236-10 2010 With amlodipine after ET-1, we obtained vasodilation and abolition of the vasospasm. Amlodipine 5-15 endothelin-1 Oryctolagus cuniculus 22-26 19921236-11 2010 CONCLUSIONS: Our study has two main conclusions: (1) amlodipine, an L-type calcium channel blocker, caused intense vasodilation and decreased both frequency and amplitude of the spontaneous oscillations observed in the rabbit external ophthalmic artery and its collaterals, and (2) when we applied amlodipine in arteries previously contracted by the administration of ET-1, vascular resistance greatly decreased and spontaneous oscillations were abolished. Amlodipine 53-63 endothelin-1 Oryctolagus cuniculus 368-372 19770190-0 2009 Activation of native TRPC1/C5/C6 channels by endothelin-1 is mediated by both PIP3 and PIP2 in rabbit coronary artery myocytes. Phosphatidylinositol 4,5-Diphosphate 87-91 endothelin-1 Oryctolagus cuniculus 45-57 19847759-0 2009 Effect of a chloride channel inhibitor, 5-nitro-2- (3-phenylpropylamino)-benzoate, on endothelin-1 induced vasoconstriction in rabbit basilar artery. 5-nitro-2- (3-phenylpropylamino)-benzoate 40-81 endothelin-1 Oryctolagus cuniculus 86-98 19847759-3 2009 The aim of the present study was to test the effect of 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB), a Cl- channel antagonist, on the ET-1-induced cerebral vasospasm in rabbit basilar artery and thus investigate the contribution of Cl- channels. 5-nitro-2-(3-phenylpropylamino)-benzoate 55-95 endothelin-1 Oryctolagus cuniculus 137-141 19365024-1 2009 OBJECTIVE: To determine whether subconjunctival injection of unoprostone isopropyl alters changes in the topography and blood flow of the optic disc induced by endothelin 1 (ET-1) in rabbits. isopropyl unoprostone 61-82 endothelin-1 Oryctolagus cuniculus 160-172 20137409-5 2009 (2) Compared with control group after exposed for 30 days, the expression of ET-1 mRNA [B group:(17.39 +/- 4.59) x 104; C group: (36.21 +/- 13.13) x 104 ] were much higher and expression of eNOS mRNA [A group: (19.11 +/- 6.83) x 104; B group: (11.86 +/- 3.15) x 104; C group: (4.68 +/- 3.26) x 104] was much lower, there were significant differences (P < 0.01). Carbon 4-5 endothelin-1 Oryctolagus cuniculus 77-81 19365024-1 2009 OBJECTIVE: To determine whether subconjunctival injection of unoprostone isopropyl alters changes in the topography and blood flow of the optic disc induced by endothelin 1 (ET-1) in rabbits. isopropyl unoprostone 61-82 endothelin-1 Oryctolagus cuniculus 174-178 18691452-8 2009 Carbenoxolone inhibited ET-1-induced contraction; (2) connexin43 protein level is increased in ET-1-stimulated basilar arteries. Carbenoxolone 0-13 endothelin-1 Oryctolagus cuniculus 24-28 18691452-8 2009 Carbenoxolone inhibited ET-1-induced contraction; (2) connexin43 protein level is increased in ET-1-stimulated basilar arteries. Carbenoxolone 0-13 endothelin-1 Oryctolagus cuniculus 95-99 18691452-9 2009 Carbenoxolone decreases the connexin43 protein level increased by ET-1; (3) cells treated with ET-1 appeared positive communicate and the dye transfer was increased in a time-dependent fashion; 4 carbenoxolone suppressed the ET-1-induced increases in dye transfer. Carbenoxolone 0-13 endothelin-1 Oryctolagus cuniculus 66-70 18691452-9 2009 Carbenoxolone decreases the connexin43 protein level increased by ET-1; (3) cells treated with ET-1 appeared positive communicate and the dye transfer was increased in a time-dependent fashion; 4 carbenoxolone suppressed the ET-1-induced increases in dye transfer. Carbenoxolone 0-13 endothelin-1 Oryctolagus cuniculus 95-99 18691452-9 2009 Carbenoxolone decreases the connexin43 protein level increased by ET-1; (3) cells treated with ET-1 appeared positive communicate and the dye transfer was increased in a time-dependent fashion; 4 carbenoxolone suppressed the ET-1-induced increases in dye transfer. Carbenoxolone 0-13 endothelin-1 Oryctolagus cuniculus 95-99 18691452-9 2009 Carbenoxolone decreases the connexin43 protein level increased by ET-1; (3) cells treated with ET-1 appeared positive communicate and the dye transfer was increased in a time-dependent fashion; 4 carbenoxolone suppressed the ET-1-induced increases in dye transfer. Carbenoxolone 196-209 endothelin-1 Oryctolagus cuniculus 95-99 18691452-9 2009 Carbenoxolone decreases the connexin43 protein level increased by ET-1; (3) cells treated with ET-1 appeared positive communicate and the dye transfer was increased in a time-dependent fashion; 4 carbenoxolone suppressed the ET-1-induced increases in dye transfer. Carbenoxolone 196-209 endothelin-1 Oryctolagus cuniculus 95-99 19128383-1 2008 PURPOSE: To determine whether a topically instilled prostaglandin analogue inhibits endothelin-1 (ET-1)-induced vasoconstrictive effects in the posterior retina by its local effects, and the duration of the effect in normal rabbit eyes. Prostaglandins 52-65 endothelin-1 Oryctolagus cuniculus 84-96 19128383-1 2008 PURPOSE: To determine whether a topically instilled prostaglandin analogue inhibits endothelin-1 (ET-1)-induced vasoconstrictive effects in the posterior retina by its local effects, and the duration of the effect in normal rabbit eyes. Prostaglandins 52-65 endothelin-1 Oryctolagus cuniculus 98-102 19128383-5 2008 RESULTS: In the rabbit eyes where travoprost was instilled for 7 days, the ET-1-induced constriction of retinal vessels was significantly inhibited only on the drug-treated side, when ET-1 was injected 30 or 60 min after the final instillation (P = 0.026-0.005), which was abolished by indomethacin pretreatment. Travoprost 34-44 endothelin-1 Oryctolagus cuniculus 75-79 19128383-5 2008 RESULTS: In the rabbit eyes where travoprost was instilled for 7 days, the ET-1-induced constriction of retinal vessels was significantly inhibited only on the drug-treated side, when ET-1 was injected 30 or 60 min after the final instillation (P = 0.026-0.005), which was abolished by indomethacin pretreatment. Travoprost 34-44 endothelin-1 Oryctolagus cuniculus 184-188 19128383-5 2008 RESULTS: In the rabbit eyes where travoprost was instilled for 7 days, the ET-1-induced constriction of retinal vessels was significantly inhibited only on the drug-treated side, when ET-1 was injected 30 or 60 min after the final instillation (P = 0.026-0.005), which was abolished by indomethacin pretreatment. Indomethacin 286-298 endothelin-1 Oryctolagus cuniculus 75-79 19128383-5 2008 RESULTS: In the rabbit eyes where travoprost was instilled for 7 days, the ET-1-induced constriction of retinal vessels was significantly inhibited only on the drug-treated side, when ET-1 was injected 30 or 60 min after the final instillation (P = 0.026-0.005), which was abolished by indomethacin pretreatment. Indomethacin 286-298 endothelin-1 Oryctolagus cuniculus 184-188 19128383-7 2008 CONCLUSIONS: After a 7-day instillation in normal rabbit eyes, topical travoprost suppressed ET-1-induced vasoconstrictive effects only in the ipsilateral posterior retina by its local effect; this effect was maintained at least for 30 min and mediated by endogenous prostaglandins. Travoprost 71-81 endothelin-1 Oryctolagus cuniculus 93-97 19128383-7 2008 CONCLUSIONS: After a 7-day instillation in normal rabbit eyes, topical travoprost suppressed ET-1-induced vasoconstrictive effects only in the ipsilateral posterior retina by its local effect; this effect was maintained at least for 30 min and mediated by endogenous prostaglandins. Prostaglandins 267-281 endothelin-1 Oryctolagus cuniculus 93-97 18055509-7 2008 In intact myocytes endothelin-1 (100 nM) was used to stimulate IP(3) production and caused a 38% (P < 0.05) increase in the amplitude of action potential-induced (0.5 Hz, field stimulation) Ca(2+) transients. Inositol 1,4,5-Trisphosphate 63-68 endothelin-1 Oryctolagus cuniculus 19-31 18188139-2 2008 In this study we examined the effect of 17beta-estradiol (E2) alone and in combination with two progestins on the endothelin-1 (ET-1) system in coronary arteries. Estradiol 40-56 endothelin-1 Oryctolagus cuniculus 114-126 18302698-10 2008 BQ-485 (100 microM, endothelin-1 type A receptor blocker) reversed and prevented the chrono-inhibitory effects of endothelin-1 (10 nM). BQ 485 0-6 endothelin-1 Oryctolagus cuniculus 20-32 18302698-10 2008 BQ-485 (100 microM, endothelin-1 type A receptor blocker) reversed and prevented the chrono-inhibitory effects of endothelin-1 (10 nM). BQ 485 0-6 endothelin-1 Oryctolagus cuniculus 114-126 18302698-11 2008 Endothelin-1 (10 nM) reduced the L-type calcium currents, transient outward currents, delayed rectifier currents, transient inward currents, and sodium-calcium exchanger currents in the PV cardiomyocytes with and without pacemaker activity. Calcium 40-47 endothelin-1 Oryctolagus cuniculus 0-12 18302698-11 2008 Endothelin-1 (10 nM) reduced the L-type calcium currents, transient outward currents, delayed rectifier currents, transient inward currents, and sodium-calcium exchanger currents in the PV cardiomyocytes with and without pacemaker activity. Sodium 145-151 endothelin-1 Oryctolagus cuniculus 0-12 18302698-11 2008 Endothelin-1 (10 nM) reduced the L-type calcium currents, transient outward currents, delayed rectifier currents, transient inward currents, and sodium-calcium exchanger currents in the PV cardiomyocytes with and without pacemaker activity. Calcium 152-159 endothelin-1 Oryctolagus cuniculus 0-12 18302698-12 2008 Endothelin-1 (10 nM) increased the inward rectifier potassium current, hyperpolarization-induced pacemaker current, and the sustained outward potassium current in PV cardiomyocytes with and without pacemaker activity. Potassium 52-61 endothelin-1 Oryctolagus cuniculus 0-12 18078923-4 2008 YM598 (10(-7)-10(-5) M) antagonized these endothelin-1-induced contractile responses without affecting the maximal responses. N-(6-methoxy-5-(2-methoxyphenoxy)-2-(pyrimidin-2-yl)pyrimidin-4-yl)-2-phenylethenesulfonamide 0-5 endothelin-1 Oryctolagus cuniculus 42-54 17612974-6 2007 These results indicate that endothelin-1 increases intracellular calcium concentration in rabbit suprachoroidal tissue via endothelin-B receptors on melanocytes. Calcium 65-72 endothelin-1 Oryctolagus cuniculus 28-40 17760634-0 2007 Neuroprotective effect of topically applied brimonidine tartrate 0.2% in endothelin-1-induced optic nerve ischaemia model. Brimonidine Tartrate 44-64 endothelin-1 Oryctolagus cuniculus 73-85 17360961-6 2007 EDN1 decreased (P </= 0.01) progesterone release and increased (P </= 0.01) PGF2alpha secretion and NOS activity via the PLC/PKC pathway in Day-9 CL, but not in Day-4 CL, cultured in vitro. Progesterone 31-43 endothelin-1 Oryctolagus cuniculus 0-4 17360961-6 2007 EDN1 decreased (P </= 0.01) progesterone release and increased (P </= 0.01) PGF2alpha secretion and NOS activity via the PLC/PKC pathway in Day-9 CL, but not in Day-4 CL, cultured in vitro. Dinoprost 82-91 endothelin-1 Oryctolagus cuniculus 0-4 17360961-7 2007 EDN1-induced, but not alfaprostol-induced luteolysis, was blocked by cotreatment in vivo with the ACE antagonist captopril. Captopril 113-122 endothelin-1 Oryctolagus cuniculus 0-4 17360961-8 2007 These findings support the hypothesis that PGF2alpha regulates luteolysis through intraluteal activation of the renin-angiotensin/EDN1 systems in CL that have acquired luteolytic competence. Dinoprost 43-52 endothelin-1 Oryctolagus cuniculus 130-134 17459374-5 2007 Levobunolol relaxed ciliary artery rings that were pre-contracted with either high-K solution, 1microM histamine, 10microM phenylephrine, or 100nM endothelin-1. Levobunolol 0-11 endothelin-1 Oryctolagus cuniculus 147-159 17552879-0 2008 Nitric oxide and prostaglandins - important players in endothelin-1 induced myocardial distensibility. Nitric Oxide 0-12 endothelin-1 Oryctolagus cuniculus 55-67 17552879-0 2008 Nitric oxide and prostaglandins - important players in endothelin-1 induced myocardial distensibility. Prostaglandins 17-31 endothelin-1 Oryctolagus cuniculus 55-67 18068544-4 2008 A significant impairment of the endothelium-dependent relaxation (EDR) caused by acetylcholine was found in 20 cases (43.5%) out of 46 SAH animals, and the same animals exhibited accompanying the significantly impaired cyclic GMP production, accumulated endogenous NOS inhibitors, attenuated DDAH activity, enhanced arginase activity and accumulated ET-1 within the vessel wall. Acetylcholine 81-94 endothelin-1 Oryctolagus cuniculus 350-354 17658500-7 2007 NSC(MS) facilitation by ET-1 was prevented by BQ-123 (1 microM, an ET(A) antagonist) but not by BQ-788 (1 microM, an ET(B) antagonist). cyclo(Trp-Asp-Pro-Val-Leu) 46-52 endothelin-1 Oryctolagus cuniculus 24-28 17658500-9 2007 ET-1- or PMA-induced facilitation of NSC(MS) was abolished by GF109203X (10 microM), a protein kinase C inhibitor. bisindolylmaleimide I 62-71 endothelin-1 Oryctolagus cuniculus 0-4 17658500-11 2007 Treatment with ET-1 (10 pM) augmented the myogenic response and this was inhibited by DIDS (30 microM). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 86-90 endothelin-1 Oryctolagus cuniculus 15-19 17612974-0 2007 Endothelin-B receptors on suprachoroidal melanocytes mediate an endothelin-1-induced increase in the intracellular calcium concentration of rabbit ocular suprachoroidal tissue. Calcium 115-122 endothelin-1 Oryctolagus cuniculus 64-76 17612974-1 2007 The physiological properties of endothelin-1 in ocular choroidal melanocytes are not well-known, although endothelin-1 increases the intracellular calcium concentration through endothelin-B receptors in skin melanocytes. Calcium 147-154 endothelin-1 Oryctolagus cuniculus 106-118 17612974-3 2007 Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue. Fura-2 0-6 endothelin-1 Oryctolagus cuniculus 44-56 17612974-3 2007 Fura-2 microfluorophotometry indicated that endothelin-1 and endothelin-B receptor selective agonists, endothelin-3 and BQ3020, increased the intracellular calcium concentration of the tissue. Calcium 156-163 endothelin-1 Oryctolagus cuniculus 44-56 17578707-0 2007 Acute cocaine induces endothelin-1-dependent constriction of rabbit basilar artery. Cocaine 6-13 endothelin-1 Oryctolagus cuniculus 22-34 17578707-3 2007 This study tested whether cocaine constriction was mediated via endothelin-1. Cocaine 26-33 endothelin-1 Oryctolagus cuniculus 64-76 17578707-6 2007 These results support the hypothesis that constriction of the cerebral vasculature due to acute cocaine exposure is via endothelin-1 release. Cocaine 96-103 endothelin-1 Oryctolagus cuniculus 120-132 17657165-4 2007 Using confocal imaging of Fluo-3 loaded in thein situ VSMC within the intact IVC, we found that ET1 elicited [Ca2+]i oscillations with an average frequency of 0.31 +/- 0.01 Hz. Fluo-3 26-32 endothelin-1 Oryctolagus cuniculus 96-99 17303636-4 2007 ET-1-induced responses were inhibited by the ET(A) receptor antagonist BQ123 and the phospholipase C (PLC) inhibitor U73122. cyclo(Trp-Asp-Pro-Val-Leu) 71-76 endothelin-1 Oryctolagus cuniculus 0-4 17303636-4 2007 ET-1-induced responses were inhibited by the ET(A) receptor antagonist BQ123 and the phospholipase C (PLC) inhibitor U73122. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 117-123 endothelin-1 Oryctolagus cuniculus 0-4 17303636-5 2007 The diacylglycerol analogue 1-oleoyl-2-acetyl-sn-glycerol (OAG) also stimulated I(cat), but the protein kinase C (PKC) inhibitor chelerythrine did not inhibit either the OAG- or ET-1-induced I(cat). 1-oleoyl-2-acetylglycerol 59-62 endothelin-1 Oryctolagus cuniculus 178-182 17364744-8 2007 In the analyses, the ET-1 induced alterations were normalized to the values in the vehicle treated control eyes, i.e., kallidinogenase (K) + ET-1/K+ vehicle or saline (S) +ET-1/S + vehicle. Sodium Chloride 160-166 endothelin-1 Oryctolagus cuniculus 21-25 17364744-12 2007 The relative number of RGCs was decreased in the saline-injected group, and this decrease was also decreased by kallidinogenase (P < 0.05, t test, K + ET-1/K+ vehicle vs. S +ET-1/S + vehicle). Sodium Chloride 49-55 endothelin-1 Oryctolagus cuniculus 154-158 17364744-12 2007 The relative number of RGCs was decreased in the saline-injected group, and this decrease was also decreased by kallidinogenase (P < 0.05, t test, K + ET-1/K+ vehicle vs. S +ET-1/S + vehicle). Sodium Chloride 49-55 endothelin-1 Oryctolagus cuniculus 177-181 17657165-4 2007 Using confocal imaging of Fluo-3 loaded in thein situ VSMC within the intact IVC, we found that ET1 elicited [Ca2+]i oscillations with an average frequency of 0.31 +/- 0.01 Hz. vsmc 54-58 endothelin-1 Oryctolagus cuniculus 96-99 17657165-10 2007 Thus, we conclude that ET1 stimulates tonic contraction in the rabbit IVC by inducing [Ca2+]i oscillations and that stimulated Ca2+ entry through both the L-type voltage-gated Ca2+ channels and a nifedipine-resistant and SKF96365-sensitive pathway is crucial for the maintenance of [Ca2+]i oscillations and tonic contraction. Nifedipine 196-206 endothelin-1 Oryctolagus cuniculus 23-26 17657165-10 2007 Thus, we conclude that ET1 stimulates tonic contraction in the rabbit IVC by inducing [Ca2+]i oscillations and that stimulated Ca2+ entry through both the L-type voltage-gated Ca2+ channels and a nifedipine-resistant and SKF96365-sensitive pathway is crucial for the maintenance of [Ca2+]i oscillations and tonic contraction. 1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole 221-229 endothelin-1 Oryctolagus cuniculus 23-26 16162868-3 2005 This finding suggested that the increase in [Ca(2+)](i) induced by ET-1 was attributable to release of Ca(2+) from ryanodine- and inositol 1,4,5-trisphosphate-sensitive intracellular Ca(2+) stores. Ryanodine 115-124 endothelin-1 Oryctolagus cuniculus 67-71 16967374-2 2006 Decoy oligodesoxynucleotides (ODN) against the transcription factor activator protein-1 (AP-1) inhibits pre-pro-endothelin-1 expression. decoy oligodesoxynucleotides 0-28 endothelin-1 Oryctolagus cuniculus 112-124 16732986-10 2006 The expressions of GATA-4 protein and phosphorylation were enhanced significantly in groups induced with ET-1 combined with 5-aza (P < 0.05). Azacitidine 124-129 endothelin-1 Oryctolagus cuniculus 105-109 16612843-3 2006 Endothelium removal or N(G)-nitro-L-arginine (L-NOARG) enhanced contractions to endothelin-1 either in control and diabetic arteries. Nitroarginine 23-44 endothelin-1 Oryctolagus cuniculus 80-92 16612843-3 2006 Endothelium removal or N(G)-nitro-L-arginine (L-NOARG) enhanced contractions to endothelin-1 either in control and diabetic arteries. Nitroarginine 46-53 endothelin-1 Oryctolagus cuniculus 80-92 16612843-4 2006 Indomethacin inhibited endothelin-1-induced response in control arteries, but enhanced it in diabetic arteries. Indomethacin 0-12 endothelin-1 Oryctolagus cuniculus 23-35 16612843-5 2006 In contrast to that observed in rubbed and in L-NOARG treated arteries, in the presence of indomethacin the contractile action of endothelin-1 was higher in diabetic arteries than in control arteries. Nitroarginine 46-53 endothelin-1 Oryctolagus cuniculus 130-142 16612843-5 2006 In contrast to that observed in rubbed and in L-NOARG treated arteries, in the presence of indomethacin the contractile action of endothelin-1 was higher in diabetic arteries than in control arteries. Indomethacin 91-103 endothelin-1 Oryctolagus cuniculus 130-142 16612843-6 2006 Nimesulide enhanced endothelin-1 contractions both in control and diabetic arteries. nimesulide 0-10 endothelin-1 Oryctolagus cuniculus 20-32 16612843-7 2006 Cyclo-(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123, endothelin ET(A) receptor antagonist), attenuated endothelin-1 vasoconstriction in control rabbits, while vasoconstriction resulted increased in diabetic rabbits. emodepside 0-7 endothelin-1 Oryctolagus cuniculus 93-105 16612843-7 2006 Cyclo-(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123, endothelin ET(A) receptor antagonist), attenuated endothelin-1 vasoconstriction in control rabbits, while vasoconstriction resulted increased in diabetic rabbits. -asp-pro-d-val-leu-d-trp 8-32 endothelin-1 Oryctolagus cuniculus 93-105 16612843-7 2006 Cyclo-(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123, endothelin ET(A) receptor antagonist), attenuated endothelin-1 vasoconstriction in control rabbits, while vasoconstriction resulted increased in diabetic rabbits. cyclo(Trp-Asp-Pro-Val-Leu) 35-41 endothelin-1 Oryctolagus cuniculus 93-105 16612843-9 2006 In summary, diabetes decreases the sensitivity of the rabbit renal artery to endothelin-1 by decreasing the ratio between vasoconstrictor and vasodilator prostanoids released after activation of endothelin ET(A) receptors. Prostaglandins 154-165 endothelin-1 Oryctolagus cuniculus 77-89 16634525-10 2006 BQ123 10(-5) M significantly inhibited ET-1-mediated CSM contractions more than BQ788 10(-5) M (both ANOVA p<0.01). cyclo(Trp-Asp-Pro-Val-Leu) 0-5 endothelin-1 Oryctolagus cuniculus 39-43 16325476-3 2006 Sildenafil (10 nM-0.1 mM) induced concentration-dependent relaxations of endothelin-1 (10 nM)-precontracted basilar artery, which were partially inhibited both in endothelium-denuded arteries and in arteries precontracted by depolarization with KCl (50 mM). Sildenafil Citrate 0-10 endothelin-1 Oryctolagus cuniculus 73-85 16325476-3 2006 Sildenafil (10 nM-0.1 mM) induced concentration-dependent relaxations of endothelin-1 (10 nM)-precontracted basilar artery, which were partially inhibited both in endothelium-denuded arteries and in arteries precontracted by depolarization with KCl (50 mM). Potassium Chloride 245-248 endothelin-1 Oryctolagus cuniculus 73-85 16325476-4 2006 Endothelin-1 (1 pM-30 nM) induced concentration-dependent contractions that were inhibited by sildenafil (0.1-100 microM). Sildenafil Citrate 94-104 endothelin-1 Oryctolagus cuniculus 0-12 16313280-5 2005 ET-1 also stimulated activation of ERK1/2 and the activation was inhibited by BQ-123, an ETA inhibitor, and TAK044, an ETA/ETB inhibitor, but not by BQ-788, an ETB inhibitor. cyclo(Trp-Asp-Pro-Val-Leu) 78-84 endothelin-1 Oryctolagus cuniculus 0-4 16313280-6 2005 ET-1-stimulated proliferation was inhibited by PD98059 or U0126. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 47-54 endothelin-1 Oryctolagus cuniculus 0-4 16313280-6 2005 ET-1-stimulated proliferation was inhibited by PD98059 or U0126. U 0126 58-63 endothelin-1 Oryctolagus cuniculus 0-4 16253233-5 2005 Endothelin-1 (1-31) also caused ocular hypertension, which was inhibited by a selective endothelin ET(A) receptor antagonist, cyclo(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123). emodepside 126-131 endothelin-1 Oryctolagus cuniculus 0-12 16253233-5 2005 Endothelin-1 (1-31) also caused ocular hypertension, which was inhibited by a selective endothelin ET(A) receptor antagonist, cyclo(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123). d-asp-pro-d-val-leu-d-trp 132-157 endothelin-1 Oryctolagus cuniculus 0-12 16220076-5 2005 The inhibition of Kir currents by ET-1 was abolished by pretreatment with the protein kinase C (PKC) inhibitor staurosporine (100 nM) or GF 109203X (1 microm). Staurosporine 111-124 endothelin-1 Oryctolagus cuniculus 34-38 16220076-5 2005 The inhibition of Kir currents by ET-1 was abolished by pretreatment with the protein kinase C (PKC) inhibitor staurosporine (100 nM) or GF 109203X (1 microm). bisindolylmaleimide I 137-147 endothelin-1 Oryctolagus cuniculus 34-38 16220076-7 2005 The ETA-receptor inhibitor BQ-123 prevented the ET-1-induced inhibition of Kir currents. cyclo(Trp-Asp-Pro-Val-Leu) 27-33 endothelin-1 Oryctolagus cuniculus 48-52 16842775-8 2006 Losartan completely blocked the inotropic and lusitropic effects of angiotensin-II, while the attenuation of these effects after the selective removal of the endocardial endothelium was blunted by concomitant administration of endothelin-1 (Protocol F). Losartan 0-8 endothelin-1 Oryctolagus cuniculus 227-239 17219836-1 2006 OBJECT: Endothelin 1 (ET-1) is a major cause of cerebral vasospasm after subarachnoid hemorrhage (SAH), and extracellular Cal++ influx plays an essential role in ET-1-induced vasospasm. palmatine 122-127 endothelin-1 Oryctolagus cuniculus 8-20 17219836-1 2006 OBJECT: Endothelin 1 (ET-1) is a major cause of cerebral vasospasm after subarachnoid hemorrhage (SAH), and extracellular Cal++ influx plays an essential role in ET-1-induced vasospasm. palmatine 122-127 endothelin-1 Oryctolagus cuniculus 22-26 17219836-1 2006 OBJECT: Endothelin 1 (ET-1) is a major cause of cerebral vasospasm after subarachnoid hemorrhage (SAH), and extracellular Cal++ influx plays an essential role in ET-1-induced vasospasm. palmatine 122-127 endothelin-1 Oryctolagus cuniculus 162-166 17219836-3 2006 In the present study, they investigate the effects of phospholipase C (PLC) on ET-1-induced activation of these Ca++ channels and BA contraction by using the PLC inhibitor U73122. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 172-178 endothelin-1 Oryctolagus cuniculus 79-83 17219836-7 2006 The U73122 inhibited the ET-1-induced transient increase in [Ca++]i, which resulted from mobilization of Ca++ from the intracellular store. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 4-10 endothelin-1 Oryctolagus cuniculus 25-29 17219836-9 2006 The U73122 inhibited the ET-1-induced contraction of the rabbit BA rings, which depended on extracellular Cal++ influx through the SOCC and NSCC-2. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 4-10 endothelin-1 Oryctolagus cuniculus 25-29 17219836-9 2006 The U73122 inhibited the ET-1-induced contraction of the rabbit BA rings, which depended on extracellular Cal++ influx through the SOCC and NSCC-2. Barium 64-66 endothelin-1 Oryctolagus cuniculus 25-29 17219836-9 2006 The U73122 inhibited the ET-1-induced contraction of the rabbit BA rings, which depended on extracellular Cal++ influx through the SOCC and NSCC-2. palmatine 106-111 endothelin-1 Oryctolagus cuniculus 25-29 17219836-13 2006 (2) The PLC is involved in the ET-1-induced contraction of rabbit BA rings, which depends on extracellular Ca++ influx through the SOCC and NSCC-2. Barium 66-68 endothelin-1 Oryctolagus cuniculus 31-35 16162868-3 2005 This finding suggested that the increase in [Ca(2+)](i) induced by ET-1 was attributable to release of Ca(2+) from ryanodine- and inositol 1,4,5-trisphosphate-sensitive intracellular Ca(2+) stores. Inositol 1,4,5-Trisphosphate 130-158 endothelin-1 Oryctolagus cuniculus 67-71 16162868-6 2005 Using the nystatin-perforated patch-clamp technique, we found that IRL-1620 caused an increase in Ca(2+) current that was inhibited by addition of ET-1. Nystatin 10-18 endothelin-1 Oryctolagus cuniculus 147-151 16162868-10 2005 Finally, U-73122 inhibited the ET-1-induced [Ca(2+)](i) increase, indicating that phospholipase C was involved in modulation of the ET-1-induced [Ca(2+)](i) increase in rabbit pulmonary artery smooth muscle cells. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 9-16 endothelin-1 Oryctolagus cuniculus 31-35 16162868-10 2005 Finally, U-73122 inhibited the ET-1-induced [Ca(2+)](i) increase, indicating that phospholipase C was involved in modulation of the ET-1-induced [Ca(2+)](i) increase in rabbit pulmonary artery smooth muscle cells. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 9-16 endothelin-1 Oryctolagus cuniculus 132-136 15860558-3 2005 Because endothelin (ET)-1 also regulates epididymal motility, we tested its sex steroid dependence in a rabbit model. Steroids 80-87 endothelin-1 Oryctolagus cuniculus 8-25 16207427-0 2005 [The expression levels of endothelin-1 and nuclear factor-kappaB in the lung tissue of acute pulmonary embolism and the effects of thrombolysis and dexamethasone]. Dexamethasone 148-161 endothelin-1 Oryctolagus cuniculus 26-64 16207427-1 2005 OBJECTIVE: To study the expression levels of endothelin-1(ET-1) and nuclear factor-kappaB (NF-kappaB) in lung vascular endothelium, bronchial and alveolar epithelia in acute pulmonary thromboembolism (APTE), and to explore the effects of thrombolytic (urokinase, UK) or ant-inflammatory therapy (dexamethasone, Dex) on their expressions. Dexamethasone 296-309 endothelin-1 Oryctolagus cuniculus 45-57 16207427-1 2005 OBJECTIVE: To study the expression levels of endothelin-1(ET-1) and nuclear factor-kappaB (NF-kappaB) in lung vascular endothelium, bronchial and alveolar epithelia in acute pulmonary thromboembolism (APTE), and to explore the effects of thrombolytic (urokinase, UK) or ant-inflammatory therapy (dexamethasone, Dex) on their expressions. Dextromethorphan 311-314 endothelin-1 Oryctolagus cuniculus 45-57 16207427-14 2005 In the UK + Dex group, the ET-1 expression was 0.186 +/- 0.033, 0.107 +/- 0.012 and 0.098 +/- 0.026 respectively, significantly different from the PTE group (all P < 0.05); the NF-kappaB P65 expression was 0.109 +/- 0.018, 0.062 +/- 0.023 and 0.093 +/- 0.019 respectively, significantly lower as compared with the PTE and the UK groups (all P < 0. Dextromethorphan 12-15 endothelin-1 Oryctolagus cuniculus 27-31 15820090-0 2005 Effects of pinacidil on proliferation of cultured rabbit airway smooth muscle cells induced by endothelin-1. Pinacidil 11-20 endothelin-1 Oryctolagus cuniculus 95-107 15956117-4 2005 Phosphoramidon, a dual neutral endopeptidase and endothelin-converting enzyme inhibitor, blocked both pressor responses to endothelin-1 (1-31) and big endothelin-1 but not those afforded by endothelin-1. phosphoramidon 0-14 endothelin-1 Oryctolagus cuniculus 123-135 15956117-4 2005 Phosphoramidon, a dual neutral endopeptidase and endothelin-converting enzyme inhibitor, blocked both pressor responses to endothelin-1 (1-31) and big endothelin-1 but not those afforded by endothelin-1. phosphoramidon 0-14 endothelin-1 Oryctolagus cuniculus 151-163 15956117-4 2005 Phosphoramidon, a dual neutral endopeptidase and endothelin-converting enzyme inhibitor, blocked both pressor responses to endothelin-1 (1-31) and big endothelin-1 but not those afforded by endothelin-1. phosphoramidon 0-14 endothelin-1 Oryctolagus cuniculus 151-163 15956117-5 2005 Thiorphan, a neutral endopeptidase inhibitor, markedly inhibited the response to endothelin-1 (1-31) but only weakly reduced that of big endothelin-1. Thiorphan 0-9 endothelin-1 Oryctolagus cuniculus 81-93 15956117-5 2005 Thiorphan, a neutral endopeptidase inhibitor, markedly inhibited the response to endothelin-1 (1-31) but only weakly reduced that of big endothelin-1. Thiorphan 0-9 endothelin-1 Oryctolagus cuniculus 137-149 15956117-7 2005 Furthermore, injection of big endothelin-1 concomitantly with phosphoramidon induced an increase in endothelin-1 (1-31) plasma levels. phosphoramidon 62-76 endothelin-1 Oryctolagus cuniculus 30-42 15956117-7 2005 Furthermore, injection of big endothelin-1 concomitantly with phosphoramidon induced an increase in endothelin-1 (1-31) plasma levels. phosphoramidon 62-76 endothelin-1 Oryctolagus cuniculus 100-112 15956117-8 2005 Finally, intracardiac-administered endothelin-1 (1-31) induced an increase of endothelin-1 plasma levels, which are markedly reduced by phosphoramidon and thiorphan but not by CGS 35066. phosphoramidon 136-150 endothelin-1 Oryctolagus cuniculus 35-47 15956117-8 2005 Finally, intracardiac-administered endothelin-1 (1-31) induced an increase of endothelin-1 plasma levels, which are markedly reduced by phosphoramidon and thiorphan but not by CGS 35066. phosphoramidon 136-150 endothelin-1 Oryctolagus cuniculus 78-90 15956117-8 2005 Finally, intracardiac-administered endothelin-1 (1-31) induced an increase of endothelin-1 plasma levels, which are markedly reduced by phosphoramidon and thiorphan but not by CGS 35066. Thiorphan 155-164 endothelin-1 Oryctolagus cuniculus 35-47 15956117-8 2005 Finally, intracardiac-administered endothelin-1 (1-31) induced an increase of endothelin-1 plasma levels, which are markedly reduced by phosphoramidon and thiorphan but not by CGS 35066. Thiorphan 155-164 endothelin-1 Oryctolagus cuniculus 78-90 15867947-3 2005 In normal rabbit eyes, topically instilled bunazosin hydrochloride reached the posterior retina by local penetration at concentrations sufficient to attenuate the phenylephrine- or endothelin-1 (ET-1)-induced constriction of retinal arteries. bunazosin 43-66 endothelin-1 Oryctolagus cuniculus 181-193 15867947-3 2005 In normal rabbit eyes, topically instilled bunazosin hydrochloride reached the posterior retina by local penetration at concentrations sufficient to attenuate the phenylephrine- or endothelin-1 (ET-1)-induced constriction of retinal arteries. bunazosin 43-66 endothelin-1 Oryctolagus cuniculus 195-199 15867947-4 2005 Furthermore, bunazosin hydrochloride improved the impairment of optic nerve head (ONH) blood flow, the prolongation of visual-evoked potentials (VEP) implicit time, the enlargement of the optic disk cup, and the decrease in the number of retinal ganglion cell layer cells induced by repeated injections of ET-1 in rabbits. bunazosin 13-36 endothelin-1 Oryctolagus cuniculus 306-310 15814466-0 2005 Amelioration of endothelin-1-induced optic nerve head ischemia by topical bunazosin. bunazosin 74-83 endothelin-1 Oryctolagus cuniculus 16-28 15792775-3 2005 Here, we explored the effects of iptakalim on the rise of cytoplasmic free Ca(2+) concentration ([Ca(2+)](cyt)) induced by endothelin-1 (ET-1) and on the proliferation of cultured rabbit pulmonary arterial SMCs. N-(1-methylethyl)-1,1,2-trimethylpropylamine 33-42 endothelin-1 Oryctolagus cuniculus 123-135 15792775-7 2005 In conclusion, we have shown that iptakalim had an inhibitory effect on [Ca(2+)](cyt) increase and the proliferation of pulmonary arterial SMCs induced by endothelin-1 through activation of K(ATP) channels. N-(1-methylethyl)-1,1,2-trimethylpropylamine 34-43 endothelin-1 Oryctolagus cuniculus 155-167 15486036-3 2005 Simvastatin has been shown to inhibit endothelin-1. Simvastatin 0-11 endothelin-1 Oryctolagus cuniculus 38-50 15486036-9 2005 The increased endothelin-1 responses can be inhibited after simvastatin administration. Simvastatin 60-71 endothelin-1 Oryctolagus cuniculus 14-26 15486036-14 2005 The results of the present study suggest that the antihypertropic and electrocardiographic effects of simvastatin at a clinical therapeutic dose are mediated through inhibition of tissue endothelin-1 expression, which is linked to mevalonate metabolism, and result in an amelioration of cardiomyocyte hypertrophy development by an atherogenic diet. Simvastatin 102-113 endothelin-1 Oryctolagus cuniculus 187-199 15486036-14 2005 The results of the present study suggest that the antihypertropic and electrocardiographic effects of simvastatin at a clinical therapeutic dose are mediated through inhibition of tissue endothelin-1 expression, which is linked to mevalonate metabolism, and result in an amelioration of cardiomyocyte hypertrophy development by an atherogenic diet. Mevalonic Acid 231-241 endothelin-1 Oryctolagus cuniculus 187-199 15591146-3 2005 Pretreatments with Bosentan, a dual ET(A)/ET(B) receptor antagonist, or cyclooxygenase (COX) inhibitor blocked the luteolytic action of ET-1 but not that induced by prostaglandin F2alpha (PGF2alpha). Bosentan 19-27 endothelin-1 Oryctolagus cuniculus 136-140 15591146-3 2005 Pretreatments with Bosentan, a dual ET(A)/ET(B) receptor antagonist, or cyclooxygenase (COX) inhibitor blocked the luteolytic action of ET-1 but not that induced by prostaglandin F2alpha (PGF2alpha). Dinoprost 188-197 endothelin-1 Oryctolagus cuniculus 136-140 15591146-4 2005 In CL cultured in vitro, ET-1 increased (P </= 0.01) both PGF(2alpha) production and luteal nitric oxide synthase activity but decreased (P < or = 0.01) progesterone release. Prostaglandins F 61-64 endothelin-1 Oryctolagus cuniculus 25-29 15591146-4 2005 In CL cultured in vitro, ET-1 increased (P </= 0.01) both PGF(2alpha) production and luteal nitric oxide synthase activity but decreased (P < or = 0.01) progesterone release. Progesterone 159-171 endothelin-1 Oryctolagus cuniculus 25-29 15591146-5 2005 Addition of ET(A) receptor antagonist BQ123 or COX inhibitor blocked the ET-1 luteolytic effects. cyclo(Trp-Asp-Pro-Val-Leu) 38-43 endothelin-1 Oryctolagus cuniculus 73-77 15591146-11 2005 Our data suggest that the luteolytic action of ET-1 may be a result of PGF2alpha synthesis from both luteal and accessory cells, via the COX pathways. Dinoprost 71-80 endothelin-1 Oryctolagus cuniculus 47-51 15900512-0 2005 Differential inhibition by the Rho kinase inhibitor Y-27632 of the increases in contractility and Ca2+ transients induced by endothelin-1 in rabbit ventricular myocytes. Y 27632 52-59 endothelin-1 Oryctolagus cuniculus 125-137 15900512-2 2005 To study the potential role of Rho kinase in the regulation of cardiac contractility and Ca(2+) transients induced by endothelin-1 (ET-1) and isoproterenol, we used the Rho kinase inhibitor Y-27632 in rabbit ventricular myocardium and myocytes loaded with indo-1/AM. Y 27632 190-197 endothelin-1 Oryctolagus cuniculus 118-130 15900512-2 2005 To study the potential role of Rho kinase in the regulation of cardiac contractility and Ca(2+) transients induced by endothelin-1 (ET-1) and isoproterenol, we used the Rho kinase inhibitor Y-27632 in rabbit ventricular myocardium and myocytes loaded with indo-1/AM. Y 27632 190-197 endothelin-1 Oryctolagus cuniculus 132-136 15900512-4 2005 Furthermore, Y-27632 suppressed the increase in contractility and Ca(2+) transients induced by ET-1 in a concentration-dependent manner, when it was used in a concentration at which it did not affect the effects of isoproterenol via beta-adrenoceptors. Y 27632 13-20 endothelin-1 Oryctolagus cuniculus 95-99 15900512-5 2005 In the presence of Y-27632, ET-1 increased cell shortening in the absence of an increase in Ca(2+) transients. Y 27632 19-26 endothelin-1 Oryctolagus cuniculus 28-32 15900512-6 2005 This is an indication that the increase in myofilament Ca(2+) sensitivity induced by ET-1 is less susceptible to the inhibitory action of Y-27632. Y 27632 138-145 endothelin-1 Oryctolagus cuniculus 85-89 15814466-1 2005 PURPOSE: To investigate the effects of bunazosin hydrochloride, an alpha1-adrenergic blocker, on the impairment of optic nerve head (ONH) blood flow and depression of visual function induced by repeated intravitreal injections of endothelin-1 (ET-1) in rabbits. bunazosin 39-62 endothelin-1 Oryctolagus cuniculus 230-242 15814466-8 2005 CONCLUSIONS: These results indicate that in rabbits, topical bunazosin suppresses the changes in ONH circulation and function induced by intravitreal ET-1. bunazosin 61-70 endothelin-1 Oryctolagus cuniculus 150-154 15634387-0 2004 [Effects of iptakalim on the proliferation of cultured rabbit pulmonary arterial smooth muscle cells induced by endothelin-1]. N-(1-methylethyl)-1,1,2-trimethylpropylamine 12-21 endothelin-1 Oryctolagus cuniculus 112-124 15654257-1 2005 We investigated the effects of the vasoconstrictor endothelin-1 (ET-1) on the whole-cell ATP-sensitive K+ (KATP) currents of smooth muscle cells that were isolated enzymatically from rabbit coronary artery (CASMCs) and pulmonary artery (PASMCs). Adenosine Triphosphate 89-92 endothelin-1 Oryctolagus cuniculus 51-63 15659303-0 2005 Wortmannin inhibits the myofilament Ca2+ sensitization induced by endothelin-1. Wortmannin 0-10 endothelin-1 Oryctolagus cuniculus 66-78 15659303-2 2005 We carried out the experiments to examine the potential contribution of myosin light chain kinase to the Ca2+ sensitization induced by endothelin-1 by use of wortmannin that inhibits myosin light chain kinase at high concentrations (IC50=200 nM). Wortmannin 158-168 endothelin-1 Oryctolagus cuniculus 135-147 15659303-4 2005 Wortmannin at 1 and 3 microM markedly inhibited the positive inotropic effect of endothelin-1, but did not affect the increase in Ca2+ transients elicited by endothelin-1. Wortmannin 0-10 endothelin-1 Oryctolagus cuniculus 81-93 15505054-0 2004 Effects of topically instilled bunazosin, an alpha1-adrenoceptor antagonist, on constrictions induced by phenylephrine and ET-1 in rabbit retinal arteries. bunazosin 31-40 endothelin-1 Oryctolagus cuniculus 123-127 15505054-16 2004 CONCLUSIONS: The present findings suggest that topically instilled bunazosin reaches the posterior retina by local penetration at concentrations sufficient to attenuate the phenylephrine- or ET-1-induced constriction of retinal arteries in normal rabbit eyes, and that the inhibitory effect of bunazosin on the ET-1-induced vasoconstriction in this tissue may be partly attributable to an interaction between the alpha1-adrenoceptor and ET receptor. bunazosin 67-76 endothelin-1 Oryctolagus cuniculus 191-195 15505054-16 2004 CONCLUSIONS: The present findings suggest that topically instilled bunazosin reaches the posterior retina by local penetration at concentrations sufficient to attenuate the phenylephrine- or ET-1-induced constriction of retinal arteries in normal rabbit eyes, and that the inhibitory effect of bunazosin on the ET-1-induced vasoconstriction in this tissue may be partly attributable to an interaction between the alpha1-adrenoceptor and ET receptor. bunazosin 67-76 endothelin-1 Oryctolagus cuniculus 311-315 15838260-0 2004 Chloride efflux is involved in ET-1 and 5-HT-induced contraction in rabbit basilar artery. Chlorides 0-8 endothelin-1 Oryctolagus cuniculus 31-41 15258766-0 2004 Extracellular Mg(2+) blocks endothelin-1-induced contraction through the inhibition of non-selective cation channels in coronary smooth muscle. magnesium ion 14-20 endothelin-1 Oryctolagus cuniculus 28-40 15258766-1 2004 This study investigated the effects of changing the extracellular [Mg(2+)] ([Mg(2+)](o)) on endothelin-1 (ET-1)-induced contraction of rabbit coronary artery smooth muscle and the involvement of non-selective cation (NSC) channels in this response. magnesium ion 67-73 endothelin-1 Oryctolagus cuniculus 92-104 15258766-1 2004 This study investigated the effects of changing the extracellular [Mg(2+)] ([Mg(2+)](o)) on endothelin-1 (ET-1)-induced contraction of rabbit coronary artery smooth muscle and the involvement of non-selective cation (NSC) channels in this response. magnesium ion 77-83 endothelin-1 Oryctolagus cuniculus 92-104 15258766-2 2004 Increased [Mg(2+)](o) shifted the concentration/contraction relationship curve of ET-1 to the right. magnesium ion 11-17 endothelin-1 Oryctolagus cuniculus 82-86 15258766-3 2004 In whole-cell patch clamp recordings, ET-1 (10(-7) M) induced a long-lasting inwards current (94.7+/-7.2 pA) that was inhibited by 8 mM [Mg(2+)](o) (45.3+/-4.4%) and NSC channel blockers (10(-3) M streptomycin and 10(-3) M La(3+)), but not by the voltage-dependent Ca(2+) channel blocker nicardipine. Streptomycin 197-209 endothelin-1 Oryctolagus cuniculus 38-42 15258766-3 2004 In whole-cell patch clamp recordings, ET-1 (10(-7) M) induced a long-lasting inwards current (94.7+/-7.2 pA) that was inhibited by 8 mM [Mg(2+)](o) (45.3+/-4.4%) and NSC channel blockers (10(-3) M streptomycin and 10(-3) M La(3+)), but not by the voltage-dependent Ca(2+) channel blocker nicardipine. Nicardipine 288-299 endothelin-1 Oryctolagus cuniculus 38-42 15258766-5 2004 Furthermore, in pressurized arteries, the ET-1-induced contraction was also inhibited by La(3+) and streptomycin, but not by nicardipine. lanthanum(3+) 89-95 endothelin-1 Oryctolagus cuniculus 42-46 15258766-5 2004 Furthermore, in pressurized arteries, the ET-1-induced contraction was also inhibited by La(3+) and streptomycin, but not by nicardipine. Streptomycin 100-112 endothelin-1 Oryctolagus cuniculus 42-46 15258766-6 2004 U-73122, a selective phospholipase C (PLC) inhibitor and staurosporine and GF 109203X, which block protein kinase C (PKC), reduced ET-1-activated NSC currents by 54.2+/-5.1%, 60.3+/-5.5% and 48.5+/-2.9%, respectively. 1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione 0-7 endothelin-1 Oryctolagus cuniculus 131-135 15258766-6 2004 U-73122, a selective phospholipase C (PLC) inhibitor and staurosporine and GF 109203X, which block protein kinase C (PKC), reduced ET-1-activated NSC currents by 54.2+/-5.1%, 60.3+/-5.5% and 48.5+/-2.9%, respectively. Staurosporine 57-70 endothelin-1 Oryctolagus cuniculus 131-135 15258766-6 2004 U-73122, a selective phospholipase C (PLC) inhibitor and staurosporine and GF 109203X, which block protein kinase C (PKC), reduced ET-1-activated NSC currents by 54.2+/-5.1%, 60.3+/-5.5% and 48.5+/-2.9%, respectively. bisindolylmaleimide I 75-85 endothelin-1 Oryctolagus cuniculus 131-135 15258766-8 2004 Like transient receptor potential channel (TRPC3) currents, ET-1-activated NSC currents had a linear I/V relationship, were blocked by flufenamate and activated by a diacylglycerol analogue. Flufenamic Acid 135-146 endothelin-1 Oryctolagus cuniculus 60-64 15258766-8 2004 Like transient receptor potential channel (TRPC3) currents, ET-1-activated NSC currents had a linear I/V relationship, were blocked by flufenamate and activated by a diacylglycerol analogue. Diglycerides 166-180 endothelin-1 Oryctolagus cuniculus 60-64 15258766-9 2004 These results suggest that [Mg(2+)](o) blocks ET-1-induced contraction of coronary arteries by inhibiting NSC channels. magnesium ion 28-34 endothelin-1 Oryctolagus cuniculus 46-50 15634387-1 2004 OBJECTIVE: To explore the effects of iptakalim on the proliferation of rabbit pulmonary arterial smooth muscle cell(PASMC) induced by endothelin-1(ET-1) in vitro. N-(1-methylethyl)-1,1,2-trimethylpropylamine 37-46 endothelin-1 Oryctolagus cuniculus 134-146 15492768-5 2004 In anesthetized rabbits, lomerizine and the other Ca(2+) channel blockers increased the ocular circulation and also inhibited the hypoperfusion induced in optic nerve head tissue by an intravitreous injection of endothelin-1. lomerizine 25-35 endothelin-1 Oryctolagus cuniculus 212-224 15172678-0 2004 Effect of Iloprost on endothelin-1-induced free radical activation in rabbit brain stem. Iloprost 10-18 endothelin-1 Oryctolagus cuniculus 22-34 15225667-4 2004 Testosterone and nandrolone significantly reduced HDL-cholesterol levels, potentiated vasoconstriction responses to epinephrine, serotonin and endothelin-1, and attenuated vasorelaxant responses to sodium nitroprusside in rabbits. Testosterone 0-12 endothelin-1 Oryctolagus cuniculus 143-155 15225667-4 2004 Testosterone and nandrolone significantly reduced HDL-cholesterol levels, potentiated vasoconstriction responses to epinephrine, serotonin and endothelin-1, and attenuated vasorelaxant responses to sodium nitroprusside in rabbits. Nandrolone 17-27 endothelin-1 Oryctolagus cuniculus 143-155 15486769-0 2004 Effects of topically instilled bunazosin hydrochloride and other ocular hypotensive drugs on endothelin-1-induced constriction in rabbit retinal arteries. bunazosin 31-54 endothelin-1 Oryctolagus cuniculus 93-105 15193993-2 2004 In this study, we investigated the effects of phosphoinositide 3-kinase (PI3K) on ET-1-induced activation of these channels and BA contraction by using PI3K inhibitors, wortmannin and LY 249002. Wortmannin 169-179 endothelin-1 Oryctolagus cuniculus 82-86 15193993-2 2004 In this study, we investigated the effects of phosphoinositide 3-kinase (PI3K) on ET-1-induced activation of these channels and BA contraction by using PI3K inhibitors, wortmannin and LY 249002. Lysine 184-186 endothelin-1 Oryctolagus cuniculus 82-86 15193993-5 2004 Only NSCC-1 was activated by ET-1 in wortmannin- or LY 294002-pretreated VSMCs. Wortmannin 37-47 endothelin-1 Oryctolagus cuniculus 29-33 15193993-5 2004 Only NSCC-1 was activated by ET-1 in wortmannin- or LY 294002-pretreated VSMCs. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 52-61 endothelin-1 Oryctolagus cuniculus 29-33 15193993-5 2004 Only NSCC-1 was activated by ET-1 in wortmannin- or LY 294002-pretreated VSMCs. vsmcs 73-78 endothelin-1 Oryctolagus cuniculus 29-33 15193993-7 2004 Wortmannin inhibited the ET-1-induced contraction of rabbit BA rings that depends on the Ca(2+) influx through NSCC-2 and SOCC. Wortmannin 0-10 endothelin-1 Oryctolagus cuniculus 25-29 15193993-8 2004 The IC(50) values of wortmannin for the ET-1-induced Ca(2+) influx and vasoconstriction were similar to those for the ET-1-induced PI3K activation. Wortmannin 21-31 endothelin-1 Oryctolagus cuniculus 40-44 15172678-1 2004 Iloprost, a stable analogue of prostacyclin, was used to reverse the early period of vasoconstriction provoked by Endothelin-1 by administering into the rabbit basilar artery. Iloprost 0-8 endothelin-1 Oryctolagus cuniculus 114-126 15172678-1 2004 Iloprost, a stable analogue of prostacyclin, was used to reverse the early period of vasoconstriction provoked by Endothelin-1 by administering into the rabbit basilar artery. Epoprostenol 31-43 endothelin-1 Oryctolagus cuniculus 114-126 15178368-0 2004 Differential inhibition by TAK-044 of the inotropic effects of endothelin-1 and endothelin-3. TAK 044 27-34 endothelin-1 Oryctolagus cuniculus 63-75 15117817-4 2004 Affs from Ang II 200 rabbits had increased (P<0.01) mRNA for COX-2 and enhanced vasoconstriction to Ang II, U-46 619 (TP-R mimetic), and endothelin-1 that was normalized by ifetroban plus tempol together. ifetroban 176-185 endothelin-1 Oryctolagus cuniculus 140-152 15117817-4 2004 Affs from Ang II 200 rabbits had increased (P<0.01) mRNA for COX-2 and enhanced vasoconstriction to Ang II, U-46 619 (TP-R mimetic), and endothelin-1 that was normalized by ifetroban plus tempol together. tempol 191-197 endothelin-1 Oryctolagus cuniculus 140-152 15200122-5 2004 METHODS: Mitogen-activated protein kinase inhibitor, PD98059, inhibited ET-1-induced ERK1/2 stimulation in rabbit basilar artery (BA) vascular smooth-muscle cells (VSMCs). 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 53-60 endothelin-1 Oryctolagus cuniculus 72-76 15200122-6 2004 Moreover, PD98059 inhibited ET-1-induced contraction of rabbit BA rings. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 10-17 endothelin-1 Oryctolagus cuniculus 28-32 15200122-7 2004 A specific inhibitor of EGFR PTK, AG1478, inhibited ET-1-induced EGFR-PTK transactivation, ERK1/2 stimulation, and contraction of BA rings in a concentration-dependent manner. RTKI cpd 34-40 endothelin-1 Oryctolagus cuniculus 52-56 15200122-8 2004 The concentration of AG1478 required for 50% inhibition of the ET-1-induced contraction of BA rings was similar to that for ET-1-induced EGFR-PTK transactivation. RTKI cpd 21-27 endothelin-1 Oryctolagus cuniculus 63-67 15200122-9 2004 Furthermore, AG1478 also inhibited ET-1-induced BA vasospasm in vivo. RTKI cpd 13-19 endothelin-1 Oryctolagus cuniculus 35-39 15178368-4 2004 In a receptor-binding assay, TAK-044 was four times more potent in antagonizing the specific binding of endothelin-1 than that of endothelin-3. TAK 044 29-36 endothelin-1 Oryctolagus cuniculus 104-116 15178368-5 2004 Endothelin-1 may activate receptor subtypes that trigger both positive and negative inotropic effects, the latter being more susceptible to the antagonistic action of TAK-044, which may explain in part the differential antagonistic action of TAK-044 on the inotropic effect of endothelin-1 and endothelin-3. TAK 044 167-174 endothelin-1 Oryctolagus cuniculus 0-12 15178368-5 2004 Endothelin-1 may activate receptor subtypes that trigger both positive and negative inotropic effects, the latter being more susceptible to the antagonistic action of TAK-044, which may explain in part the differential antagonistic action of TAK-044 on the inotropic effect of endothelin-1 and endothelin-3. TAK 044 167-174 endothelin-1 Oryctolagus cuniculus 277-289 15178368-5 2004 Endothelin-1 may activate receptor subtypes that trigger both positive and negative inotropic effects, the latter being more susceptible to the antagonistic action of TAK-044, which may explain in part the differential antagonistic action of TAK-044 on the inotropic effect of endothelin-1 and endothelin-3. TAK 044 242-249 endothelin-1 Oryctolagus cuniculus 0-12 15178368-2 2004 While TAK-044 produced a concentration-dependent rightward shift of the concentration-response curve for endothelin-1 and endothelin-3, the effect of endothelin-3 was hundred times more sensitive to TAK-044 than that of endothelin-1. TAK 044 6-13 endothelin-1 Oryctolagus cuniculus 105-117 15259279-6 2004 RESULTS: Treatment with MPA caused a significant increase in vasoconstriction, expressed as E(max) (mN/mm, mean +/- SEM; p < 0.05), in response to potassium (3.18 +/- 0.19 vs. 2.47 +/- 0.19) and calcium (4.00 +/- 0.22 vs. 3.34 +/- 0.14) in the posterior cerebral artery, and to endothelin-1 (6.88 +/- 0.69 vs. 5.22 +/- 0.30) in the basilar artery, when compared with NETA. Potassium 150-159 endothelin-1 Oryctolagus cuniculus 281-293 14751546-2 2004 ET-1 induced inositol phosphates production, MAP kinase phosphorylation, MLC phosphorylation (MLC20-P plus MLC20-2P) and contraction in a concentration-dependent manner with EC50 values of 71, 8, 6 and 25 nM, respectively. Phosphates 22-32 endothelin-1 Oryctolagus cuniculus 0-4 14751546-3 2004 ET-1-induced MAP kinase phosphorylation, MLC phosphorylation and contraction were not significantly affected by nifedipine (1-60 microM), an L-type Ca2+ channel blocker, or by LOE 908 (1-100 microM), a blocker of Ca2+-permeable nonselective cation channels. 1-[[(3~{S})-1,4-dioxaspiro[4.5]decan-3-yl]methyl]piperidine 176-179 endothelin-1 Oryctolagus cuniculus 0-4 14751546-5 2004 2-APB, a store-operated Ca2+ channel (SOCC) blocker, inhibited ET-1-induced MLC phosphorylation and contraction in a concentration-dependent manner with IC50 values of 12.7 and 19 microM, respectively, but was without effect on MAP kinase phosphorylation. 2-aminoethoxydiphenyl borate 0-5 endothelin-1 Oryctolagus cuniculus 63-67 14751546-6 2004 The combined effects of submaximal concentrations of SKF96365 and 2-APB on ET-1-induced MLC phosphorylation and contraction were not additive, implying that their inhibitory actions could be mediated through a common Ca2+ entry channel. 1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole 53-61 endothelin-1 Oryctolagus cuniculus 75-79 14751546-6 2004 The combined effects of submaximal concentrations of SKF96365 and 2-APB on ET-1-induced MLC phosphorylation and contraction were not additive, implying that their inhibitory actions could be mediated through a common Ca2+ entry channel. 2-aminoethoxydiphenyl borate 66-71 endothelin-1 Oryctolagus cuniculus 75-79 14985051-1 2004 The influence of alloxan-induced diabetes on the reactivity of rabbit basilar artery to endothelin-1 was examined. Alloxan 17-24 endothelin-1 Oryctolagus cuniculus 88-100 14985051-4 2004 N(G)-nitro-L-arginine (L-NOArg) enhanced the maximal contraction induced by endothelin-1 in control rabbits and potentiated this response in diabetic rabbits. Nitroarginine 0-21 endothelin-1 Oryctolagus cuniculus 76-88 14985051-8 2004 These results suggest that mechanisms underlying rabbit basilar artery hyperreactivity to endothelin-1 include decreased endothelial modulation of endothelin-1-induced contraction, with impaired endothelial endothelin ETB receptor activity; decreased sensitivity to nitric oxide (NO) in vascular smooth muscle; and enhanced participation of muscular endothelin ETA and ETB receptors. Nitric Oxide 266-278 endothelin-1 Oryctolagus cuniculus 90-102 14985051-4 2004 N(G)-nitro-L-arginine (L-NOArg) enhanced the maximal contraction induced by endothelin-1 in control rabbits and potentiated this response in diabetic rabbits. Nitroarginine 23-30 endothelin-1 Oryctolagus cuniculus 76-88 14985051-5 2004 Endothelin ETA receptor antagonist, cyclo(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123), inhibited endothelin-1-induced contraction in both rabbit groups. emodepside 36-41 endothelin-1 Oryctolagus cuniculus 89-101 14985051-5 2004 Endothelin ETA receptor antagonist, cyclo(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123), inhibited endothelin-1-induced contraction in both rabbit groups. d-asp-pro-d-val-leu-d-trp 42-67 endothelin-1 Oryctolagus cuniculus 89-101 14985051-5 2004 Endothelin ETA receptor antagonist, cyclo(D-Asp-Pro-D-Val-Leu-D-Trp) (BQ-123), inhibited endothelin-1-induced contraction in both rabbit groups. cyclo(Trp-Asp-Pro-Val-Leu) 70-76 endothelin-1 Oryctolagus cuniculus 89-101 14985051-6 2004 Endothelin ETB receptor antagonist, 2,6-Dimethylpiperidinecarbonyl-gamma-Methyl-Leu-Nin-(Methoxycarbonyl)-D-Trp-D-Nle (BQ-788), enhanced endothelin-1-induced contraction in control rabbits and decreased the potency of endothelin-1 in diabetic rabbits. BQ 788 119-125 endothelin-1 Oryctolagus cuniculus 137-149 14985051-6 2004 Endothelin ETB receptor antagonist, 2,6-Dimethylpiperidinecarbonyl-gamma-Methyl-Leu-Nin-(Methoxycarbonyl)-D-Trp-D-Nle (BQ-788), enhanced endothelin-1-induced contraction in control rabbits and decreased the potency of endothelin-1 in diabetic rabbits. BQ 788 119-125 endothelin-1 Oryctolagus cuniculus 218-230 14500131-5 2004 ET-1 (0.1 nM) alone did not produce any significant contraction, but it markedly potentiated the magnitude (223% of control) and rate (149% of control) of contraction in response to OxyHb, which was attenuated by the inhibitors of Rho kinase Y-27632 and HA-1077. histidinoalanine 254-256 endothelin-1 Oryctolagus cuniculus 0-4 14751407-5 2004 2,6-Dimethylpiperidinecarbonyl-gamma-methyl-Leu-N(in)-(methoxycarbonyl)-D-Trp-D-Nle (BQ-788; endothelin ET(B) receptor antagonist) enhanced the contraction elicited by endothelin-1 in control arteries and displaced to the right the contractile curve for endothelin-1 in diabetic arteries. 2,6-dimethylpiperidinecarbonyl-gamma-methyl-leu-n 0-49 endothelin-1 Oryctolagus cuniculus 168-180 14751407-5 2004 2,6-Dimethylpiperidinecarbonyl-gamma-methyl-Leu-N(in)-(methoxycarbonyl)-D-Trp-D-Nle (BQ-788; endothelin ET(B) receptor antagonist) enhanced the contraction elicited by endothelin-1 in control arteries and displaced to the right the contractile curve for endothelin-1 in diabetic arteries. 2,6-dimethylpiperidinecarbonyl-gamma-methyl-leu-n 0-49 endothelin-1 Oryctolagus cuniculus 254-266 14751407-5 2004 2,6-Dimethylpiperidinecarbonyl-gamma-methyl-Leu-N(in)-(methoxycarbonyl)-D-Trp-D-Nle (BQ-788; endothelin ET(B) receptor antagonist) enhanced the contraction elicited by endothelin-1 in control arteries and displaced to the right the contractile curve for endothelin-1 in diabetic arteries. )-d-trp-d-nle 70-83 endothelin-1 Oryctolagus cuniculus 168-180 14751407-5 2004 2,6-Dimethylpiperidinecarbonyl-gamma-methyl-Leu-N(in)-(methoxycarbonyl)-D-Trp-D-Nle (BQ-788; endothelin ET(B) receptor antagonist) enhanced the contraction elicited by endothelin-1 in control arteries and displaced to the right the contractile curve for endothelin-1 in diabetic arteries. )-d-trp-d-nle 70-83 endothelin-1 Oryctolagus cuniculus 254-266 14751407-6 2004 In summary, diabetes induces hyperreactivity of the rabbit carotid artery to endothelin-1 by a mechanism that at least includes: (1) enhanced activity of muscular endothelin ET(A) receptors; (2) impairment of endothelin ET(B) receptor-mediated nitric oxide (NO) release; and (3) enhancement of the production of thromboxane A(2). Nitric Oxide 244-256 endothelin-1 Oryctolagus cuniculus 77-89 14751407-6 2004 In summary, diabetes induces hyperreactivity of the rabbit carotid artery to endothelin-1 by a mechanism that at least includes: (1) enhanced activity of muscular endothelin ET(A) receptors; (2) impairment of endothelin ET(B) receptor-mediated nitric oxide (NO) release; and (3) enhancement of the production of thromboxane A(2). thromboxane a 312-325 endothelin-1 Oryctolagus cuniculus 77-89 14751407-3 2004 Endothelium removal or N(G)-nitro-L-arginine enhanced contractions in response to endothelin-1 only in control arteries, without modifying the endothelin-1 response in diabetic arteries. Nitroarginine 23-44 endothelin-1 Oryctolagus cuniculus 82-94 14500131-6 2004 ET-1-mediated potentiation of the contraction was also inhibited by inhibitors of PKC, Ro-32-0432, and GF-109203X. Ro 32-0432 87-97 endothelin-1 Oryctolagus cuniculus 0-4 14500131-6 2004 ET-1-mediated potentiation of the contraction was also inhibited by inhibitors of PKC, Ro-32-0432, and GF-109203X. glycylphenylalanine 103-105 endothelin-1 Oryctolagus cuniculus 0-4 14500131-7 2004 BQ-123 prevented potentiation of vasoconstriction mediated by ET-1, indicating that the action of ET-1 was mediated by the endothelin type A receptor. cyclo(Trp-Asp-Pro-Val-Leu) 0-6 endothelin-1 Oryctolagus cuniculus 62-66 14500131-7 2004 BQ-123 prevented potentiation of vasoconstriction mediated by ET-1, indicating that the action of ET-1 was mediated by the endothelin type A receptor. cyclo(Trp-Asp-Pro-Val-Leu) 0-6 endothelin-1 Oryctolagus cuniculus 98-102 14678242-10 2003 After vehicle, renal arterial infusions of noradrenaline, angiotensin II and endothelin-1 reduced RBF and cortical laser Doppler flux (CLDF), but not MLDF. cldf 135-139 endothelin-1 Oryctolagus cuniculus 77-89 14678242-10 2003 After vehicle, renal arterial infusions of noradrenaline, angiotensin II and endothelin-1 reduced RBF and cortical laser Doppler flux (CLDF), but not MLDF. mldf 150-154 endothelin-1 Oryctolagus cuniculus 77-89 12910401-0 2003 Intra-arterial simultaneous administration of anandamide attenuates endothelin-1 induced vasospasm in rabbit basilar arteries. anandamide 46-56 endothelin-1 Oryctolagus cuniculus 68-80 14508240-1 2003 Endothelin-1 reduces the chronotropic and inotropic effects of the beta-adrenoceptor agonist isoproterenol in rabbit isolated atria. Isoproterenol 93-106 endothelin-1 Oryctolagus cuniculus 0-12 14508240-8 2003 In the presence of endothelin-1, chelerythrine (protein kinase C inhibitor; 10 micromol/L) increased (P < 0.05) vascular sensitivity to isoproterenol (8.6 +/- 0.4, n = 7), but had no influence on the Emax. chelerythrine 33-46 endothelin-1 Oryctolagus cuniculus 19-31 14508240-8 2003 In the presence of endothelin-1, chelerythrine (protein kinase C inhibitor; 10 micromol/L) increased (P < 0.05) vascular sensitivity to isoproterenol (8.6 +/- 0.4, n = 7), but had no influence on the Emax. Isoproterenol 139-152 endothelin-1 Oryctolagus cuniculus 19-31 14508240-11 2003 In conclusion, endothelin-1 reduces vascular sensitivity to isoproterenol in a PKC-dependent pathway. Isoproterenol 60-73 endothelin-1 Oryctolagus cuniculus 15-27 12910401-3 2003 The purpose of this study was to investigate whether anandamide inhibits the acute vascular and morphological effects of Endothelin-1 applied intra-arterially on rabbit basilar arteries. anandamide 53-63 endothelin-1 Oryctolagus cuniculus 121-133 12910401-7 2003 FINDINGS: Angiographic studies showed that simultaneous administration of anandamide significantly attenuated Endothelin-1 induced vasoconstriction. anandamide 74-84 endothelin-1 Oryctolagus cuniculus 110-122 12910401-8 2003 Furthermore, it was demonstrated that anandamide reversed the morphological changes induced by Endothelin-1 on the vessel wall. anandamide 38-48 endothelin-1 Oryctolagus cuniculus 95-107 12650428-3 2003 Moreover, the receptor-operated Ca++ channel blocker LOE 908 inhibits ET-1-induced extracellular Ca++ influx via NSCCs in the VSMCs of the basilar artery (BA) and the NSCC-dependent part of ET-1-induced vasoconstriction of BA rings. 1-[[(3~{S})-1,4-dioxaspiro[4.5]decan-3-yl]methyl]piperidine 53-56 endothelin-1 Oryctolagus cuniculus 190-194 12646747-0 2003 Evaluation of nitric oxide synthesis in the optic nerve head in vivo using microdialysis and high-performance liquid chromatography and its interaction with endothelin-1. Nitric Oxide 14-26 endothelin-1 Oryctolagus cuniculus 157-169 12646747-1 2003 PURPOSE: To investigate the relationship between nitric oxide synthesis and endothelin-1 (ET-1) in the optic nerve head in vivo. Nitric Oxide 49-61 endothelin-1 Oryctolagus cuniculus 76-88 12660490-0 2003 Unoprostone isopropyl pretreatment decreases endothelin-1 release and the intra-ocular pressure spike induced by laser trabeculoplasty in the rabbit. isopropyl unoprostone 0-21 endothelin-1 Oryctolagus cuniculus 45-57 12660490-1 2003 PURPOSE: We have previously shown that the intra-ocular pressure (IOP) spike due to argon laser trabeculoplasty (ALT) is caused by an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour of rabbit eyes. Argon 84-89 endothelin-1 Oryctolagus cuniculus 140-152 12660490-1 2003 PURPOSE: We have previously shown that the intra-ocular pressure (IOP) spike due to argon laser trabeculoplasty (ALT) is caused by an acute endothelin-1 (ET-1) release from the uveal tissue into the aqueous humour of rabbit eyes. Argon 84-89 endothelin-1 Oryctolagus cuniculus 154-158 12660490-2 2003 In this study we investigated whether pretreatment with topical unoprostone isopropyl, a functional antagonist of ET-1, protects against the pressure spike due to ALT in the rabbit model. isopropyl unoprostone 64-85 endothelin-1 Oryctolagus cuniculus 114-118 12644581-0 2003 Involvements of voltage-independent Ca2+ channels and phosphoinositide 3-kinase in endothelin-1-induced PYK2 tyrosine phosphorylation. Tyrosine 109-117 endothelin-1 Oryctolagus cuniculus 83-95 12644581-4 2003 The purpose of the present study was to identify the Ca(2+) channels involved in the ET-1-induced, proline-rich tyrosine kinase 2 (PYK2) phosphorylation in ICA VSMCs. isocyanic acid 156-159 endothelin-1 Oryctolagus cuniculus 85-89 12644581-5 2003 Based on sensitivity to nifedipine, an L-type voltage-operated Ca(2+) channel (VOCC) blocker, Ca(2+) influx through VOCC seems to play a minor role in the ET-1-induced PYK2 phosphorylation. Nifedipine 24-34 endothelin-1 Oryctolagus cuniculus 155-159 12644581-7 2003 The phosphoinositide 3-kinase (PI3K) inhibitors wortmannin and 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one (LY 294002), inhibited ET-1-induced Ca(2+) influx through NSCC-2 and SOCC. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 63-111 endothelin-1 Oryctolagus cuniculus 135-139 12579333-5 2003 Measurement of plasma endothelin 1 (ET-1) and nitric oxide (NO) levels showed that feeding a high-cholesterol diet significantly increased plasma ET-1 levels (from 51.4+/-17.6 to 96.9+/-24.3 pg/ml), and decreased plasma NO concentration (from 104.6+/-18.5 to 67.7+/-16.1 pg/ml). Cholesterol 98-109 endothelin-1 Oryctolagus cuniculus 22-34 12579333-5 2003 Measurement of plasma endothelin 1 (ET-1) and nitric oxide (NO) levels showed that feeding a high-cholesterol diet significantly increased plasma ET-1 levels (from 51.4+/-17.6 to 96.9+/-24.3 pg/ml), and decreased plasma NO concentration (from 104.6+/-18.5 to 67.7+/-16.1 pg/ml). Cholesterol 98-109 endothelin-1 Oryctolagus cuniculus 146-150 12579333-6 2003 With administration of resveratrol, red wine, or dealcoholized red wine, plasma ET-1 levels statistically decreased, in parallel with a significant elevation in NO levels. Resveratrol 23-34 endothelin-1 Oryctolagus cuniculus 80-84 12650428-3 2003 Moreover, the receptor-operated Ca++ channel blocker LOE 908 inhibits ET-1-induced extracellular Ca++ influx via NSCCs in the VSMCs of the basilar artery (BA) and the NSCC-dependent part of ET-1-induced vasoconstriction of BA rings. 1-[[(3~{S})-1,4-dioxaspiro[4.5]decan-3-yl]methyl]piperidine 53-56 endothelin-1 Oryctolagus cuniculus 70-74 12427604-1 2002 The purpose of this study was to demonstrate the involvement of Ca(2+) influx through voltage-independent Ca(2+) channels (VICCs) in endothelin-1 (ET-1)-induced transactivation of epidermal growth factor receptor protein tyrosine kinase (EGFR PTK) using the Ca(2+) channel blockers LOE-908 and SK&F-96365 in rabbit internal carotid artery vascular smooth muscle cells. amicloral 294-300 endothelin-1 Oryctolagus cuniculus 133-145 12605241-1 2003 Corpus cavernosum smooth muscle (CCSM) from rabbits made diabetic for 6 months as a result of alloxan injection exhibited increased sensitivity (3vs 9 nM EC(50)) and generated 20-50% greater force to endothelin-1 (ET-1) compared to CCSM from normal rabbits. Alloxan 94-101 endothelin-1 Oryctolagus cuniculus 200-212 12605241-1 2003 Corpus cavernosum smooth muscle (CCSM) from rabbits made diabetic for 6 months as a result of alloxan injection exhibited increased sensitivity (3vs 9 nM EC(50)) and generated 20-50% greater force to endothelin-1 (ET-1) compared to CCSM from normal rabbits. Alloxan 94-101 endothelin-1 Oryctolagus cuniculus 214-218 12605241-4 2003 ET-1-induced CCSM contraction is largely dependent upon Rho-kinase (ROK), since it is almost completely blocked by Y-27632 (a highly selective ROK inhibitor). Y 27632 115-122 endothelin-1 Oryctolagus cuniculus 0-4 12548074-0 2003 Cocaine-induced endothelin-1-dependent spasm in rabbit basilar artery in vivo. Cocaine 0-7 endothelin-1 Oryctolagus cuniculus 16-28 12548074-7 2003 This study demonstrates the novel finding that endothelin-1 mediates cocaine-induced cerebral vasospasm. Cocaine 69-76 endothelin-1 Oryctolagus cuniculus 47-59 12646404-5 2003 Since we previously demonstrated that constrictions to hypocapnia and isocapnic alkaline solution were endothelin-1 dependent, we tested whether the inhibition of hypocapnia- and isocapnic alkaline solution-induced constrictions following isocapnic alkaline solution was due to reduced endothelin-1 constriction. isocapnic alkaline 70-88 endothelin-1 Oryctolagus cuniculus 103-115 12646747-1 2003 PURPOSE: To investigate the relationship between nitric oxide synthesis and endothelin-1 (ET-1) in the optic nerve head in vivo. Nitric Oxide 49-61 endothelin-1 Oryctolagus cuniculus 90-94 12646747-6 2003 Intravitreal ET-1 significantly elevated the levels of nitrate to 189% of baseline 10 min after ET-1 application, which was inhibited by pretreatment of intravenous L-NAME (50 mg/kg). Nitrates 55-62 endothelin-1 Oryctolagus cuniculus 13-17 12646747-6 2003 Intravitreal ET-1 significantly elevated the levels of nitrate to 189% of baseline 10 min after ET-1 application, which was inhibited by pretreatment of intravenous L-NAME (50 mg/kg). Nitrates 55-62 endothelin-1 Oryctolagus cuniculus 96-100 12646747-6 2003 Intravitreal ET-1 significantly elevated the levels of nitrate to 189% of baseline 10 min after ET-1 application, which was inhibited by pretreatment of intravenous L-NAME (50 mg/kg). NG-Nitroarginine Methyl Ester 165-171 endothelin-1 Oryctolagus cuniculus 13-17 12646747-6 2003 Intravitreal ET-1 significantly elevated the levels of nitrate to 189% of baseline 10 min after ET-1 application, which was inhibited by pretreatment of intravenous L-NAME (50 mg/kg). NG-Nitroarginine Methyl Ester 165-171 endothelin-1 Oryctolagus cuniculus 96-100 12646747-7 2003 CONCLUSION: These results indicate that production of nitric oxide is closely connected with the ET-1 signaling pathway. Nitric Oxide 54-66 endothelin-1 Oryctolagus cuniculus 97-101 12427604-1 2002 The purpose of this study was to demonstrate the involvement of Ca(2+) influx through voltage-independent Ca(2+) channels (VICCs) in endothelin-1 (ET-1)-induced transactivation of epidermal growth factor receptor protein tyrosine kinase (EGFR PTK) using the Ca(2+) channel blockers LOE-908 and SK&F-96365 in rabbit internal carotid artery vascular smooth muscle cells. amicloral 294-300 endothelin-1 Oryctolagus cuniculus 147-151 12427604-2 2002 ET-1-induced EGFR PTK transactivation was completely inhibited by AG-1478, which is a specific inhibitor of EGFR PTK. RTKI cpd 66-73 endothelin-1 Oryctolagus cuniculus 0-4 12429339-9 2002 CONCLUSIONS: The potency of ET-1 in producing contraction on tissue strips and inducing calcium flux suggests that ET-1 might be an important mediator for modulating and maintaining corpus cavernosum smooth muscle tone. Calcium 88-95 endothelin-1 Oryctolagus cuniculus 115-119 12198330-7 2002 Both LOE 908 and SK&F 96365 inhibit endothelin-1-induced contraction in a concentration-dependent manner, and their combination abolished it. amicloral 17-23 endothelin-1 Oryctolagus cuniculus 40-52 12429339-1 2002 OBJECTIVES: To directly compare and contrast the effects of endothelin-1 (ET-1) and adrenoreceptor agonists norepinephrine and phenylephrine on eliciting calcium influx in primary rabbit corpus cavernosum cells and their ability to elicit tissue contractions. Calcium 154-161 endothelin-1 Oryctolagus cuniculus 60-72 12429339-1 2002 OBJECTIVES: To directly compare and contrast the effects of endothelin-1 (ET-1) and adrenoreceptor agonists norepinephrine and phenylephrine on eliciting calcium influx in primary rabbit corpus cavernosum cells and their ability to elicit tissue contractions. Calcium 154-161 endothelin-1 Oryctolagus cuniculus 74-78 12429339-6 2002 RESULTS: ET-1 at concentrations as low as 10 nM was sufficient to induce a transient increase of intracellular calcium in these cells. Calcium 111-118 endothelin-1 Oryctolagus cuniculus 9-13 12429339-9 2002 CONCLUSIONS: The potency of ET-1 in producing contraction on tissue strips and inducing calcium flux suggests that ET-1 might be an important mediator for modulating and maintaining corpus cavernosum smooth muscle tone. Calcium 88-95 endothelin-1 Oryctolagus cuniculus 28-32 12491787-8 2002 ET-1 accelerated the [3H]-thymidine incorporation by cultured vascular smooth muscle cells and, on the other hand, reduced TUNEL-positive cells, which was caused by the serum deprivation. Tritium 22-24 endothelin-1 Oryctolagus cuniculus 0-4 12491787-8 2002 ET-1 accelerated the [3H]-thymidine incorporation by cultured vascular smooth muscle cells and, on the other hand, reduced TUNEL-positive cells, which was caused by the serum deprivation. Thymidine 26-35 endothelin-1 Oryctolagus cuniculus 0-4 12491787-9 2002 The reduction of TUNEL-positive cells with ET-1 was blocked by ATZ1993 or BQ123 as an antagonist for ETA receptor, but unaffected by BQ788 as an antagonist for ETB receptor. ATZ1993 63-70 endothelin-1 Oryctolagus cuniculus 43-47 12491787-9 2002 The reduction of TUNEL-positive cells with ET-1 was blocked by ATZ1993 or BQ123 as an antagonist for ETA receptor, but unaffected by BQ788 as an antagonist for ETB receptor. cyclo(Trp-Asp-Pro-Val-Leu) 74-79 endothelin-1 Oryctolagus cuniculus 43-47 12207565-9 2002 Angiotensin II- and endothelin-1-induced increases in medullary perfusion were abolished by indomethacin, but indomethacin had no significant effects on responses of regional kidney perfusion to acetylcholine, bradykinin, MAHMA NONOate, noradrenaline and [Phe 2,Ile 3,Orn 8]-vasopressin. Indomethacin 92-104 endothelin-1 Oryctolagus cuniculus 20-32 12181127-4 2002 The downstream effect of ET-1-induced contraction and RhoA activity was studied in the presence of the Rho kinase inhibitor Y-27632. Y 27632 124-131 endothelin-1 Oryctolagus cuniculus 25-29 12181127-5 2002 The effect of Rho kinase inhibitor on ET-1-induced myosin light chain (MLC) phosphorylation was investigated by using urea-glycerol-PAGE immunoblotting. Urea 118-122 endothelin-1 Oryctolagus cuniculus 38-42 12181127-5 2002 The effect of Rho kinase inhibitor on ET-1-induced myosin light chain (MLC) phosphorylation was investigated by using urea-glycerol-PAGE immunoblotting. Glycerol 123-131 endothelin-1 Oryctolagus cuniculus 38-42 12181127-7 2002 Rho kinase inhibitor Y-27632 reduced the effect of ET-1 on MLC phosphorylation. Y 27632 21-28 endothelin-1 Oryctolagus cuniculus 51-55 12099999-4 2002 Acetylcholine (ACh)-induced vasodilation that is due to the release of EDRF/NO is significantly decreased, whereas big ET-1-induced vasoconstriction was increased in kidneys isolated from GLY-pretreated rabbits. Glycerol 188-191 endothelin-1 Oryctolagus cuniculus 119-123 12416272-10 2002 These effects of ET-1 were exacerbated in the presence of BQ-788 (AT increased by 82.6 +/- 17.5% and dT/dtmax by 121.3 +/- 26.6%) and inverted in the presence of BQ-123 (AT decreased by 12.8 +/- 2.7% and dT/dtmax by 16.1 +/- 3.0%). BQ 788 58-64 endothelin-1 Oryctolagus cuniculus 17-21 12416272-10 2002 These effects of ET-1 were exacerbated in the presence of BQ-788 (AT increased by 82.6 +/- 17.5% and dT/dtmax by 121.3 +/- 26.6%) and inverted in the presence of BQ-123 (AT decreased by 12.8 +/- 2.7% and dT/dtmax by 16.1 +/- 3.0%). Thymidine 101-103 endothelin-1 Oryctolagus cuniculus 17-21 12416272-10 2002 These effects of ET-1 were exacerbated in the presence of BQ-788 (AT increased by 82.6 +/- 17.5% and dT/dtmax by 121.3 +/- 26.6%) and inverted in the presence of BQ-123 (AT decreased by 12.8 +/- 2.7% and dT/dtmax by 16.1 +/- 3.0%). dtmax 104-109 endothelin-1 Oryctolagus cuniculus 17-21 12416272-10 2002 These effects of ET-1 were exacerbated in the presence of BQ-788 (AT increased by 82.6 +/- 17.5% and dT/dtmax by 121.3 +/- 26.6%) and inverted in the presence of BQ-123 (AT decreased by 12.8 +/- 2.7% and dT/dtmax by 16.1 +/- 3.0%). cyclo(Trp-Asp-Pro-Val-Leu) 162-168 endothelin-1 Oryctolagus cuniculus 17-21 12416272-10 2002 These effects of ET-1 were exacerbated in the presence of BQ-788 (AT increased by 82.6 +/- 17.5% and dT/dtmax by 121.3 +/- 26.6%) and inverted in the presence of BQ-123 (AT decreased by 12.8 +/- 2.7% and dT/dtmax by 16.1 +/- 3.0%). Thymidine 204-206 endothelin-1 Oryctolagus cuniculus 17-21 12416272-10 2002 These effects of ET-1 were exacerbated in the presence of BQ-788 (AT increased by 82.6 +/- 17.5% and dT/dtmax by 121.3 +/- 26.6%) and inverted in the presence of BQ-123 (AT decreased by 12.8 +/- 2.7% and dT/dtmax by 16.1 +/- 3.0%). dtmax 207-212 endothelin-1 Oryctolagus cuniculus 17-21 12099999-8 2002 Big ET-1, but not ET-1, responses were found to be significantly increased in kidneys isolated from GLY-pretreated rabbits. Glycerol 100-103 endothelin-1 Oryctolagus cuniculus 4-8 12099999-9 2002 This increase is attributed to the higher conversion rate of big ET-1 to ET-1 because the ET-converting enzyme (ECE) inhibitor phosphoramidon, at a concentration of 10(-6) mol/L, causes an inhibition in the response to big ET-1 by 52.6% in normal kidneys, whereas this inhibition with the same concentration of phosphoramidon was found to be significantly decreased in kidneys isolated from GLY-pretreated rabbits. phosphoramidon 127-141 endothelin-1 Oryctolagus cuniculus 65-77 12099999-9 2002 This increase is attributed to the higher conversion rate of big ET-1 to ET-1 because the ET-converting enzyme (ECE) inhibitor phosphoramidon, at a concentration of 10(-6) mol/L, causes an inhibition in the response to big ET-1 by 52.6% in normal kidneys, whereas this inhibition with the same concentration of phosphoramidon was found to be significantly decreased in kidneys isolated from GLY-pretreated rabbits. phosphoramidon 127-141 endothelin-1 Oryctolagus cuniculus 65-69 12099999-9 2002 This increase is attributed to the higher conversion rate of big ET-1 to ET-1 because the ET-converting enzyme (ECE) inhibitor phosphoramidon, at a concentration of 10(-6) mol/L, causes an inhibition in the response to big ET-1 by 52.6% in normal kidneys, whereas this inhibition with the same concentration of phosphoramidon was found to be significantly decreased in kidneys isolated from GLY-pretreated rabbits. phosphoramidon 311-325 endothelin-1 Oryctolagus cuniculus 65-77 12099999-9 2002 This increase is attributed to the higher conversion rate of big ET-1 to ET-1 because the ET-converting enzyme (ECE) inhibitor phosphoramidon, at a concentration of 10(-6) mol/L, causes an inhibition in the response to big ET-1 by 52.6% in normal kidneys, whereas this inhibition with the same concentration of phosphoramidon was found to be significantly decreased in kidneys isolated from GLY-pretreated rabbits. phosphoramidon 311-325 endothelin-1 Oryctolagus cuniculus 65-69 12099999-9 2002 This increase is attributed to the higher conversion rate of big ET-1 to ET-1 because the ET-converting enzyme (ECE) inhibitor phosphoramidon, at a concentration of 10(-6) mol/L, causes an inhibition in the response to big ET-1 by 52.6% in normal kidneys, whereas this inhibition with the same concentration of phosphoramidon was found to be significantly decreased in kidneys isolated from GLY-pretreated rabbits. Glycerol 391-394 endothelin-1 Oryctolagus cuniculus 65-77 12099999-9 2002 This increase is attributed to the higher conversion rate of big ET-1 to ET-1 because the ET-converting enzyme (ECE) inhibitor phosphoramidon, at a concentration of 10(-6) mol/L, causes an inhibition in the response to big ET-1 by 52.6% in normal kidneys, whereas this inhibition with the same concentration of phosphoramidon was found to be significantly decreased in kidneys isolated from GLY-pretreated rabbits. Glycerol 391-394 endothelin-1 Oryctolagus cuniculus 65-69 12099999-11 2002 The non-selective NO synthase inhibitor N(G)-nitro-L- arginine methyl ester (L-NAME) caused a significant potentiation in the vasoconstrictor response to ET-1 in normal isolated perfused rabbit kidneys. NG-Nitroarginine Methyl Ester 40-75 endothelin-1 Oryctolagus cuniculus 154-158 12099999-11 2002 The non-selective NO synthase inhibitor N(G)-nitro-L- arginine methyl ester (L-NAME) caused a significant potentiation in the vasoconstrictor response to ET-1 in normal isolated perfused rabbit kidneys. NG-Nitroarginine Methyl Ester 77-83 endothelin-1 Oryctolagus cuniculus 154-158 12099999-15 2002 Increased conversion of big ET-1 to ET-1 may also contribute to the mechanism of vascular damage due to GLY. Glycerol 104-107 endothelin-1 Oryctolagus cuniculus 28-40 12193145-12 2002 Naf reduces binding of ET-1 to rabbit detrusor ET(A) and ET(B) receptors and inhibits ET-1-induced detrusor contractions mediated by ET(A) receptors. Nafronyl 0-3 endothelin-1 Oryctolagus cuniculus 23-27 12193145-12 2002 Naf reduces binding of ET-1 to rabbit detrusor ET(A) and ET(B) receptors and inhibits ET-1-induced detrusor contractions mediated by ET(A) receptors. Nafronyl 0-3 endothelin-1 Oryctolagus cuniculus 86-90 12193135-2 2002 Endothelin-converting enzyme-1 (ECE-1), the protease involved in the final step of post-translational processing of ET-1, cleaves the inactive precursor big ET-1 at the Trp(21)-Val (22) peptide bond. Tryptophan 169-172 endothelin-1 Oryctolagus cuniculus 116-120 11884501-6 2002 On the contrary, (125)I-labeled endothelin-1--bound receptor mediated to superfused aortas from untreated NZW (12 +/- 9 amol.mm(-2)) and to balloon-denuded aortas from cholesterol-fed NZW (19 +/- 5 amol.mm(-2)) but not to aortas from WHHL. Cholesterol 168-179 endothelin-1 Oryctolagus cuniculus 32-44 12193135-2 2002 Endothelin-converting enzyme-1 (ECE-1), the protease involved in the final step of post-translational processing of ET-1, cleaves the inactive precursor big ET-1 at the Trp(21)-Val (22) peptide bond. Tryptophan 169-172 endothelin-1 Oryctolagus cuniculus 157-161 12152120-4 2002 We essentially found that yohimbine not only blocks contractions induced by adrenergic agonists, but also by non-adrenergic substances, such as ET-1. Yohimbine 26-35 endothelin-1 Oryctolagus cuniculus 144-148 12152120-6 2002 Conversely, our data suggest that yohimbine counteracts ET-1-induced contractions by interfering with NO release from the endothelium. Yohimbine 34-43 endothelin-1 Oryctolagus cuniculus 56-60 12193145-0 2002 Reduction of endothelin-1 binding and inhibition of endothelin-1-mediated detrusor contraction by naftidrofuryl. Nafronyl 98-111 endothelin-1 Oryctolagus cuniculus 52-64 12193145-4 2002 The purpose of this study is to assess whether Naf will reduce ET-1 binding in the rabbit detrusor muscle and to assess whether there is inhibition of ET-1-mediated detrusor contraction. Nafronyl 47-50 endothelin-1 Oryctolagus cuniculus 63-67 12193145-6 2002 In addition, ET-1 was added to the detrusor strips in the presence of the ET(A) antagonist, BQ123, and the ET(B) antagonist, BQ788, to identify the receptor subtype functionally involved. cyclo(Trp-Asp-Pro-Val-Leu) 92-97 endothelin-1 Oryctolagus cuniculus 13-17 12193145-7 2002 Overall inhibition of [(125)I]ET-1 binding by Naf was assessed using autoradiography. Nafronyl 46-49 endothelin-1 Oryctolagus cuniculus 30-34 12193145-9 2002 Naf inhibited ET-1-mediated detrusor contractions significantly (P<0.04), e.g. at 10(-10) M ET-1, contraction was completely abolished by Naf. Nafronyl 0-3 endothelin-1 Oryctolagus cuniculus 14-18 12193145-9 2002 Naf inhibited ET-1-mediated detrusor contractions significantly (P<0.04), e.g. at 10(-10) M ET-1, contraction was completely abolished by Naf. Nafronyl 0-3 endothelin-1 Oryctolagus cuniculus 95-99 12193145-9 2002 Naf inhibited ET-1-mediated detrusor contractions significantly (P<0.04), e.g. at 10(-10) M ET-1, contraction was completely abolished by Naf. Nafronyl 141-144 endothelin-1 Oryctolagus cuniculus 14-18 12193145-9 2002 Naf inhibited ET-1-mediated detrusor contractions significantly (P<0.04), e.g. at 10(-10) M ET-1, contraction was completely abolished by Naf. Nafronyl 141-144 endothelin-1 Oryctolagus cuniculus 95-99 12193145-10 2002 Autoradiography indicated that Naf competitively inhibited [(125)I]ET-1 binding in a dose-dependent manner (IC(50)=3x10(-7) M). Nafronyl 31-34 endothelin-1 Oryctolagus cuniculus 67-71 12131560-2 2002 Ca2+ influx through these channels plays an essential role for ET-1-induced mitogenesis in ICA VSMCs. isocyanic acid 91-94 endothelin-1 Oryctolagus cuniculus 63-67 12780972-11 2002 In the lavage fluid of saline-instilled pups, ET-1 remained low and no increase in Big ET-1 levels was observed. Sodium Chloride 23-29 endothelin-1 Oryctolagus cuniculus 46-50 12780972-17 2002 In general, we conclude, that ET-1 levels are significantly elevated in meconium-instilled rabbits compared with saline-instilled ones, and both can be significantly inhibited by pretreatment with captopril. Captopril 197-206 endothelin-1 Oryctolagus cuniculus 30-34 12099541-1 2002 We investigated the effect of topical betaxolol on impaired choroidal blood flow (CBF) induced by endothelin-1 (ET-1) injection into the vitreous of albino rabbits. Betaxolol 38-47 endothelin-1 Oryctolagus cuniculus 98-110 12099541-1 2002 We investigated the effect of topical betaxolol on impaired choroidal blood flow (CBF) induced by endothelin-1 (ET-1) injection into the vitreous of albino rabbits. Betaxolol 38-47 endothelin-1 Oryctolagus cuniculus 112-116 12099541-6 2002 Compared with topical BSS, topical betaxolol significantly inhibited the decrease in CBF at 2 hrs (p = .022), 12 hrs (p = .046) and 24 hrs (p = .015) after the intravitreal injection of ET-1. Betaxolol 35-44 endothelin-1 Oryctolagus cuniculus 186-190 12099541-8 2002 The decrease in CBF after the intravitreal injection of ET-1 was partially inhibited by topical betaxolol. Betaxolol 96-105 endothelin-1 Oryctolagus cuniculus 56-60 11861335-16 2002 The non-selective ET-1 receptor antagonist, SB209670, reduced basal PAP (from 16.9 mmHg to 15.9 mmHg, P < 0.05) in rabbits with PHT and blocked the response to ET-1 in the presence of L-NAME. 1H-Indene-2-carboxylic acid, 1-(1,3-benzodioxol-5-yl)-3-(2- (carboxymethoxy)-4-methoxyphenyl)-2,3-dihydro-5-propoxy-, (1S,2R,3S)- 44-52 endothelin-1 Oryctolagus cuniculus 18-22 11861335-16 2002 The non-selective ET-1 receptor antagonist, SB209670, reduced basal PAP (from 16.9 mmHg to 15.9 mmHg, P < 0.05) in rabbits with PHT and blocked the response to ET-1 in the presence of L-NAME. 1H-Indene-2-carboxylic acid, 1-(1,3-benzodioxol-5-yl)-3-(2- (carboxymethoxy)-4-methoxyphenyl)-2,3-dihydro-5-propoxy-, (1S,2R,3S)- 44-52 endothelin-1 Oryctolagus cuniculus 163-167 11861335-16 2002 The non-selective ET-1 receptor antagonist, SB209670, reduced basal PAP (from 16.9 mmHg to 15.9 mmHg, P < 0.05) in rabbits with PHT and blocked the response to ET-1 in the presence of L-NAME. NG-Nitroarginine Methyl Ester 187-193 endothelin-1 Oryctolagus cuniculus 18-22 11861335-16 2002 The non-selective ET-1 receptor antagonist, SB209670, reduced basal PAP (from 16.9 mmHg to 15.9 mmHg, P < 0.05) in rabbits with PHT and blocked the response to ET-1 in the presence of L-NAME. NG-Nitroarginine Methyl Ester 187-193 endothelin-1 Oryctolagus cuniculus 163-167 11748114-6 2001 This twitch prolongation was completely reversed by simultaneous administration of BQ-123 and indomethacin to block endogenous ET-1 and prostaglandins, respectively. cyclo(Trp-Asp-Pro-Val-Leu) 83-89 endothelin-1 Oryctolagus cuniculus 127-131 11748114-6 2001 This twitch prolongation was completely reversed by simultaneous administration of BQ-123 and indomethacin to block endogenous ET-1 and prostaglandins, respectively. Indomethacin 94-106 endothelin-1 Oryctolagus cuniculus 127-131 11748114-7 2001 In addition, in the LPS+12h group, myocardial inotropic responsiveness to exogenous ET-1 was enhanced (P<0.01). 12-hydroxydodecanoic acid 24-27 endothelin-1 Oryctolagus cuniculus 84-88 11417311-4 2001 METHODS: The myocardial effects of 1 and 10 nM of ET-1 were evaluated in isolated rabbit papillary muscles (n = 9) and human atrial trabecula from CABG patients (Krebs-Ringer; 1.8 mM CaCl2; 35 degrees C). krebs 162-167 endothelin-1 Oryctolagus cuniculus 50-54 11500050-2 2001 Moreover, ET-1 induced concentration- and time-dependent increases in cAMP concentrations in RTSM (EC(50), 58 nM; t(1/2), 2.4 min). Cyclic AMP 70-74 endothelin-1 Oryctolagus cuniculus 10-14 11500050-3 2001 Pretreatment with the AC inhibitors, SQ-22536, or 2"-5"-dideoxyadenosine, enhanced contraction to ET-1 and converted its bell-shaped tension curve into a sigmoidal one, but left contraction to carbachol and KCl unaltered. 9-(tetrahydro-2-furyl)-adenine 37-45 endothelin-1 Oryctolagus cuniculus 98-102 11500050-3 2001 Pretreatment with the AC inhibitors, SQ-22536, or 2"-5"-dideoxyadenosine, enhanced contraction to ET-1 and converted its bell-shaped tension curve into a sigmoidal one, but left contraction to carbachol and KCl unaltered. 2',5'-dideoxyadenosine 50-72 endothelin-1 Oryctolagus cuniculus 98-102 11500050-4 2001 The potent ET(B)-receptor agonists, ET-3 or sarafotoxin-c, mimicked ET-1"s effects on cAMP levels (EC(50) values 55 and 50 nM). sarafotoxin-c 44-57 endothelin-1 Oryctolagus cuniculus 68-72 11500050-4 2001 The potent ET(B)-receptor agonists, ET-3 or sarafotoxin-c, mimicked ET-1"s effects on cAMP levels (EC(50) values 55 and 50 nM). Cyclic AMP 86-90 endothelin-1 Oryctolagus cuniculus 68-72 11483700-13 2001 Arterioles that were mildly preconstricted and depolarised by 0.1-0.3 nM endothelin-1 constricted further in response to 3,4-DAP, 4-AP or correolide, but not to rAgTX2. Amifampridine 121-128 endothelin-1 Oryctolagus cuniculus 73-85 11483700-13 2001 Arterioles that were mildly preconstricted and depolarised by 0.1-0.3 nM endothelin-1 constricted further in response to 3,4-DAP, 4-AP or correolide, but not to rAgTX2. 4-Aminopyridine 130-134 endothelin-1 Oryctolagus cuniculus 73-85 11483700-13 2001 Arterioles that were mildly preconstricted and depolarised by 0.1-0.3 nM endothelin-1 constricted further in response to 3,4-DAP, 4-AP or correolide, but not to rAgTX2. correolide 138-148 endothelin-1 Oryctolagus cuniculus 73-85 11476754-0 2001 Chelerythrine and genistein inhibit the endothelin-1-induced increase in myofilament Ca(2+) sensitivity in rabbit ventricular myocytes. chelerythrine 0-13 endothelin-1 Oryctolagus cuniculus 40-52 11476754-0 2001 Chelerythrine and genistein inhibit the endothelin-1-induced increase in myofilament Ca(2+) sensitivity in rabbit ventricular myocytes. Genistein 18-27 endothelin-1 Oryctolagus cuniculus 40-52 11476754-4 2001 The selective protein kinase C inhibitor chelerythrine at 1 microM and the tyrosine kinase inhibitor genistein at 5 microM inhibited the endothelin-1-induced increase in cell shortening without significantly affecting Ca(2+) transients and the transient decrease in cell shortening and Ca(2+) transients. chelerythrine 41-54 endothelin-1 Oryctolagus cuniculus 137-149 11476754-4 2001 The selective protein kinase C inhibitor chelerythrine at 1 microM and the tyrosine kinase inhibitor genistein at 5 microM inhibited the endothelin-1-induced increase in cell shortening without significantly affecting Ca(2+) transients and the transient decrease in cell shortening and Ca(2+) transients. Genistein 101-110 endothelin-1 Oryctolagus cuniculus 137-149 11724750-12 2001 The selective tachykinin NK(2) receptor antagonist, SR48968, was effective to reduce ET-1 potentiation of EFS mediated contraction. SR 48968 52-59 endothelin-1 Oryctolagus cuniculus 85-89 11731579-5 2001 ET-1 (100 nM) increased the cycle length of action potentials (APs) from 305 +/- 15 to 388 +/- 25 ms; this effect was antagonised by the ET(A) receptor-selective antagonist BQ-123 (1 microM). cyclo(Trp-Asp-Pro-Val-Leu) 173-179 endothelin-1 Oryctolagus cuniculus 0-4 11731579-7 2001 Under exactly the same experimental conditions, ET-1 caused a positive chronotropic effect in guinea-pig SAN with a decrease of 13% in APD50, a shift of -4 mV in the take-off potential and an increase of 8% in the PMP slope. pmp 214-217 endothelin-1 Oryctolagus cuniculus 48-52 11731579-10 2001 Whereas the spontaneous pacing rate and the PMP slope were similarly decreased by ET-1 (10 nM) in both cell types, ET-1 depolarised MDP from -67 +/- 1 to -62 +/- 4 mV in spindle-shaped cells but hyperpolarised it from -73 +/- 1 to -81 +/- 3 mV in rod-shaped cells. Acetylmuramyl-Alanyl-Isoglutamine 132-135 endothelin-1 Oryctolagus cuniculus 115-119 11731579-13 2001 ET-1 decreased the high-threshold calcium current (I(CaL)) by about 50% in both cell types, without affecting its voltage dependence, and decreased the delayed rectifier K+ current (I(K)) with significant shifts (of +4.7 and +14.0 mV in spindle- and rod-shaped cells, respectively) in its voltage dependence. Calcium 34-41 endothelin-1 Oryctolagus cuniculus 0-4 11668078-5 2001 ET-1 produced a concentration-dependent contraction of the rabbit basilar artery in the normal Cl- Krebs-Henseleit bicarbonate buffer (123 mM Cl-). cl- krebs-henseleit bicarbonate 95-126 endothelin-1 Oryctolagus cuniculus 0-4 11668078-7 2001 The ET-1-induced contraction was enhanced by substitution of extracellular Cl- (10 mM) with methanesulfonic acid (113 mM). methanesulfonic acid 92-112 endothelin-1 Oryctolagus cuniculus 4-8 11454562-4 2001 The ET(A)-receptor antagonist BQ-485 shifted CRC for ET-1 to the right in parallel; however, the facilitatory response to 10(-8) M ET-1 was markedly enhanced by BQ-485 and also by the ET(B) antagonist BQ-788. bulaquine 30-32 endothelin-1 Oryctolagus cuniculus 53-57 11454562-4 2001 The ET(A)-receptor antagonist BQ-485 shifted CRC for ET-1 to the right in parallel; however, the facilitatory response to 10(-8) M ET-1 was markedly enhanced by BQ-485 and also by the ET(B) antagonist BQ-788. BQ 485 30-36 endothelin-1 Oryctolagus cuniculus 53-57 11454562-4 2001 The ET(A)-receptor antagonist BQ-485 shifted CRC for ET-1 to the right in parallel; however, the facilitatory response to 10(-8) M ET-1 was markedly enhanced by BQ-485 and also by the ET(B) antagonist BQ-788. BQ 485 30-36 endothelin-1 Oryctolagus cuniculus 131-135 11454562-4 2001 The ET(A)-receptor antagonist BQ-485 shifted CRC for ET-1 to the right in parallel; however, the facilitatory response to 10(-8) M ET-1 was markedly enhanced by BQ-485 and also by the ET(B) antagonist BQ-788. BQ 788 201-207 endothelin-1 Oryctolagus cuniculus 131-135 11454562-5 2001 The ET(A)/ET(B) antagonist TAK-044 abolished the ET-1-induced response. TAK 044 27-34 endothelin-1 Oryctolagus cuniculus 49-53 11504119-2 2001 Endothelin-1, the most potent vasoconstrictor peptide of the endothelin family, is synthesized initially as a large prepropeptide that requires multiple steps of post-translational processing for activation. prepropeptide 116-129 endothelin-1 Oryctolagus cuniculus 0-12 11435800-4 2001 In the intact rabbit basilar artery, hydroxyfasudil elicited a concentration-dependent relaxation of the artery precontracted with 1 nmol/L endothelin-1 (ET-1) plus 20 mmol/L KCl without any significant decrease in [Ca2+]i as determined by fura-2 microfluorometry (IC50: 5.1 +/- 4.6 micromol/L). hydroxyfasudil 37-51 endothelin-1 Oryctolagus cuniculus 140-152 11435800-4 2001 In the intact rabbit basilar artery, hydroxyfasudil elicited a concentration-dependent relaxation of the artery precontracted with 1 nmol/L endothelin-1 (ET-1) plus 20 mmol/L KCl without any significant decrease in [Ca2+]i as determined by fura-2 microfluorometry (IC50: 5.1 +/- 4.6 micromol/L). hydroxyfasudil 37-51 endothelin-1 Oryctolagus cuniculus 154-158 11435800-6 2001 In the permeabilized preparation, hydroxyfasudil inhibited the contraction induced by ET-1, guanosine 5"-O-(3-thiotriphosphate), or the catalytic subunit of rho-associated kinase, but it did not inhibit Ca2+-induced contraction under the condition of inhibited myosin light chain phosphatase. hydroxyfasudil 34-48 endothelin-1 Oryctolagus cuniculus 86-90 11330876-0 2001 Angiotensin II and serotonin potentiate endothelin-1-induced vascular smooth muscle cell proliferation. Serotonin 19-28 endothelin-1 Oryctolagus cuniculus 40-52 11324981-0 2001 Effects of lomerizine, a novel Ca2+ channel blocker, on the normal and endothelin-1-disturbed circulation in the optic nerve head of rabbits. lomerizine 11-21 endothelin-1 Oryctolagus cuniculus 71-83 11679200-0 2000 Reduced verapamil inhibition of endothelin-1-constricted rabbit basilar artery due to enhanced non L-type Ca(2+)-channel-dependent constriction. Verapamil 8-17 endothelin-1 Oryctolagus cuniculus 32-44 11881060-7 2001 Captopril treatment led to a statistically significant (p<0.01) reduction in ET1 levels compared with untreated animals, but the reduction was only about half that seen with AT1-receptor blockade. Captopril 0-9 endothelin-1 Oryctolagus cuniculus 80-83 11226400-0 2001 Endothelin-1-induced potentiation of adrenergic responses in the rabbit pulmonary artery: role of thromboxane A(2). thromboxane a 98-111 endothelin-1 Oryctolagus cuniculus 0-12 11226400-2 2001 Endothelin-1 (10(-10) M) potentiated the contractions induced by electrical stimulation and noradrenaline. Norepinephrine 92-105 endothelin-1 Oryctolagus cuniculus 0-12 11226400-6 2001 The results indicate that endothelin-1 potentiates the responses to electrical stimulation and noradrenaline by activating endothelin ET(B) receptors. Norepinephrine 95-108 endothelin-1 Oryctolagus cuniculus 26-38 11163049-3 2001 Iganidipine (0.0001% to 0.1%) was instilled into eyes with impaired ocular circulation before or after the intravitreal injection of endothelin-1, and the change in ONH blood flow was measured. iganidipine 0-11 endothelin-1 Oryctolagus cuniculus 133-145 11153628-15 2000 Furthermore, ET-1 was detected in the perfusate, which was free from ET-1 before ACh administration. Acetylcholine 81-84 endothelin-1 Oryctolagus cuniculus 13-17 11153628-21 2000 In lungs with a normal pulmonary vascular resistance, ACh administration causes vasoconstriction via the release of ET-1 and TXA2, whereas vasodilation is induced in preconstricted pulmonary vessels. Acetylcholine 54-57 endothelin-1 Oryctolagus cuniculus 116-120 10942749-6 2000 Inositol phosphate responses to endothelin-1, angiotensin II, thrombin agonist peptide, and platelet-derived growth factor (PDGF) were attenuated by 37% to 72% in GRK2-overexpressing cells (P<0.01), but the response to the thromboxane A(2) analogue U46619 was unaffected. Inositol Phosphates 0-18 endothelin-1 Oryctolagus cuniculus 32-44 11341901-3 2001 (2) Iganidipine (0.0001%-0.1%) was instilled into a circulation-disordered eye before or after the intravitreal injection of endothelin-1, and change in the blood flow in the ONH was measured. iganidipine 4-15 endothelin-1 Oryctolagus cuniculus 125-137 11922964-8 2000 BQ610, BQ788, and RES-701-1 relaxed the 3-5 nM endothelin-1 constriction by only 64.3+/-7.6% (4), 43.5+/-8.5% (5), and 26.7+/-4.8% (3) (means+/-S.E. BQ 788 7-12 endothelin-1 Oryctolagus cuniculus 47-59 11113276-0 2000 Synergistic effect of endothelin-1 and serotonin in rabbit platelets: effect on tyrosine phosphorylation. Tyrosine 80-88 endothelin-1 Oryctolagus cuniculus 22-34 11078394-2 2000 Endothelin-1 (ET-1; 0.1-100.0 nM) caused graded tonic contractions with a CK50 (concentration causing response equivalent to 50% of KCl 80 mM) of 3.4 nM and EH (response to highest concentration) of 186 +/- 22, being 40-fold less potent than cholecystokinin-8 (CCK-8), 103-fold more potent than carbachol, but equipotent to ET-3, sarafotoxin S6c (S6c) and IRL 1620. Potassium Chloride 132-135 endothelin-1 Oryctolagus cuniculus 0-12 11078394-2 2000 Endothelin-1 (ET-1; 0.1-100.0 nM) caused graded tonic contractions with a CK50 (concentration causing response equivalent to 50% of KCl 80 mM) of 3.4 nM and EH (response to highest concentration) of 186 +/- 22, being 40-fold less potent than cholecystokinin-8 (CCK-8), 103-fold more potent than carbachol, but equipotent to ET-3, sarafotoxin S6c (S6c) and IRL 1620. Potassium Chloride 132-135 endothelin-1 Oryctolagus cuniculus 14-18 11078394-2 2000 Endothelin-1 (ET-1; 0.1-100.0 nM) caused graded tonic contractions with a CK50 (concentration causing response equivalent to 50% of KCl 80 mM) of 3.4 nM and EH (response to highest concentration) of 186 +/- 22, being 40-fold less potent than cholecystokinin-8 (CCK-8), 103-fold more potent than carbachol, but equipotent to ET-3, sarafotoxin S6c (S6c) and IRL 1620. cholecystokinin 8 242-259 endothelin-1 Oryctolagus cuniculus 0-12 11078394-2 2000 Endothelin-1 (ET-1; 0.1-100.0 nM) caused graded tonic contractions with a CK50 (concentration causing response equivalent to 50% of KCl 80 mM) of 3.4 nM and EH (response to highest concentration) of 186 +/- 22, being 40-fold less potent than cholecystokinin-8 (CCK-8), 103-fold more potent than carbachol, but equipotent to ET-3, sarafotoxin S6c (S6c) and IRL 1620. Cholecystokinin 261-264 endothelin-1 Oryctolagus cuniculus 0-12 11078394-2 2000 Endothelin-1 (ET-1; 0.1-100.0 nM) caused graded tonic contractions with a CK50 (concentration causing response equivalent to 50% of KCl 80 mM) of 3.4 nM and EH (response to highest concentration) of 186 +/- 22, being 40-fold less potent than cholecystokinin-8 (CCK-8), 103-fold more potent than carbachol, but equipotent to ET-3, sarafotoxin S6c (S6c) and IRL 1620. Carbachol 295-304 endothelin-1 Oryctolagus cuniculus 0-12 11015318-4 2000 For a given increase in cell shortening, the ET-1-induced increase in Ca(2+) transients was much smaller than that induced by isoprenaline (ISO, 10 nM). Isoproterenol 140-143 endothelin-1 Oryctolagus cuniculus 45-49 11015318-5 2000 Pretreatment with HOE642 and KB-R9032 (1 microM) inhibited the increase in cell shortening induced by 0.1 nM ET-1 by 51 and 65. cariporide 18-24 endothelin-1 Oryctolagus cuniculus 109-113 11015318-5 2000 Pretreatment with HOE642 and KB-R9032 (1 microM) inhibited the increase in cell shortening induced by 0.1 nM ET-1 by 51 and 65. N-(4-isopropyl-2,2-dimethyl-3-oxo-3,4-dihydro-2H-benzo(1,4)oxazine-6-carbonyl)guanidine 29-37 endothelin-1 Oryctolagus cuniculus 109-113 10979293-6 2000 Iganidipine (0.0001%-0.1%) was instilled into a circulation-disordered eye before or after the intravitreal injection of endothelin-1, and change in the blood flow in the ONH was measured. iganidipine 0-11 endothelin-1 Oryctolagus cuniculus 121-133 11679200-2 2000 Pretreatment of rabbit basilar artery in vitro with 1 microM verapamil, an L-type Ca(2+) channel blocker, inhibited 3, 10, 30, and 100 nM endothelin-1 constrictions to a lesser extent than verapamil addition during the plateau endothelin-1 constriction. Verapamil 61-70 endothelin-1 Oryctolagus cuniculus 138-150 11679200-2 2000 Pretreatment of rabbit basilar artery in vitro with 1 microM verapamil, an L-type Ca(2+) channel blocker, inhibited 3, 10, 30, and 100 nM endothelin-1 constrictions to a lesser extent than verapamil addition during the plateau endothelin-1 constriction. Verapamil 61-70 endothelin-1 Oryctolagus cuniculus 227-239 11679200-3 2000 Ni(2+) (0.03 and 0.1 mM), a nonselective cation channel blocker, relaxed the plateau endothelin-1 constrictions in vessels pretreated with verapamil to greater magnitudes than vessels unexposed to verapamil. Nickel(2+) 0-6 endothelin-1 Oryctolagus cuniculus 85-97 11679200-3 2000 Ni(2+) (0.03 and 0.1 mM), a nonselective cation channel blocker, relaxed the plateau endothelin-1 constrictions in vessels pretreated with verapamil to greater magnitudes than vessels unexposed to verapamil. Verapamil 139-148 endothelin-1 Oryctolagus cuniculus 85-97 10813381-5 2000 In saline groups, myocardial ET-1 levels were higher in shunt than in sham rabbits (217+/-22 vs. 136+/-19 pg/g tissue; p < 0.01 between rabbit groups) without changes in plasma ET-1 concentrations during saline infusion for 6 weeks. Sodium Chloride 3-9 endothelin-1 Oryctolagus cuniculus 29-33 10854619-4 2000 Under basal conditions, ET-1 and ET-3 (1.0 microM each) elevated tissue cyclic AMP (cAMP) levels nearly 3-fold (P<0.001, ET-1; P<0.05, ET-3) and inositol phosphate (IP(n)) levels nearly 4-fold (P<0.01, ET-1). Cyclic AMP 72-82 endothelin-1 Oryctolagus cuniculus 24-37 10854619-4 2000 Under basal conditions, ET-1 and ET-3 (1.0 microM each) elevated tissue cyclic AMP (cAMP) levels nearly 3-fold (P<0.001, ET-1; P<0.05, ET-3) and inositol phosphate (IP(n)) levels nearly 4-fold (P<0.01, ET-1). Cyclic AMP 84-88 endothelin-1 Oryctolagus cuniculus 24-37 10854619-4 2000 Under basal conditions, ET-1 and ET-3 (1.0 microM each) elevated tissue cyclic AMP (cAMP) levels nearly 3-fold (P<0.001, ET-1; P<0.05, ET-3) and inositol phosphate (IP(n)) levels nearly 4-fold (P<0.01, ET-1). Inositol Phosphates 151-169 endothelin-1 Oryctolagus cuniculus 24-37 10854619-4 2000 Under basal conditions, ET-1 and ET-3 (1.0 microM each) elevated tissue cyclic AMP (cAMP) levels nearly 3-fold (P<0.001, ET-1; P<0.05, ET-3) and inositol phosphate (IP(n)) levels nearly 4-fold (P<0.01, ET-1). ip 171-173 endothelin-1 Oryctolagus cuniculus 24-37 10813386-4 2000 In contrast, the Ca2+-sensitizing effect of ET-1 was significantly inhibited by either calphostin C or genistein. calphostin C 87-99 endothelin-1 Oryctolagus cuniculus 44-48 10813386-4 2000 In contrast, the Ca2+-sensitizing effect of ET-1 was significantly inhibited by either calphostin C or genistein. Genistein 103-112 endothelin-1 Oryctolagus cuniculus 44-48 10813386-1 2000 This study was undertaken to explore possible signal-transduction mechanisms involved in the Ca2+-sensitizing effects of carbachol and endothelin-1 (ET-1) by using beta-escin-skinned smooth muscle of porcine coronary artery. Escin 164-174 endothelin-1 Oryctolagus cuniculus 135-147 10813386-5 2000 Although the inhibitory effect of calphostin C on ET-1-induced Ca2+ sensitization was less than that of genistein, the effects of calphostin C and genistein were additive. calphostin C 34-46 endothelin-1 Oryctolagus cuniculus 50-54 10813386-8 2000 In beta-escin-skinned smooth muscle of rabbit mesenteric artery, ET-1-induced Ca2+ sensitization was marginally affected by C3 pretreatment, calphostin C, and genistein. Escin 3-13 endothelin-1 Oryctolagus cuniculus 65-69 10813386-8 2000 In beta-escin-skinned smooth muscle of rabbit mesenteric artery, ET-1-induced Ca2+ sensitization was marginally affected by C3 pretreatment, calphostin C, and genistein. calphostin C 141-153 endothelin-1 Oryctolagus cuniculus 65-69 10813386-8 2000 In beta-escin-skinned smooth muscle of rabbit mesenteric artery, ET-1-induced Ca2+ sensitization was marginally affected by C3 pretreatment, calphostin C, and genistein. Genistein 159-168 endothelin-1 Oryctolagus cuniculus 65-69 10813386-1 2000 This study was undertaken to explore possible signal-transduction mechanisms involved in the Ca2+-sensitizing effects of carbachol and endothelin-1 (ET-1) by using beta-escin-skinned smooth muscle of porcine coronary artery. Escin 164-174 endothelin-1 Oryctolagus cuniculus 149-153 10813386-2 2000 Pretreatment with C3 exoenzyme of Clostridium botulinum, which selectively inactivates rho p21 by adenosine diphosphate (ADP) ribosylation, resulted in a significant inhibition of ET-1-induced Ca2+ sensitization, but had no effect on carbachol-induced Ca2+ sensitization. Adenosine Diphosphate 98-119 endothelin-1 Oryctolagus cuniculus 180-184 10813386-2 2000 Pretreatment with C3 exoenzyme of Clostridium botulinum, which selectively inactivates rho p21 by adenosine diphosphate (ADP) ribosylation, resulted in a significant inhibition of ET-1-induced Ca2+ sensitization, but had no effect on carbachol-induced Ca2+ sensitization. Carbachol 234-243 endothelin-1 Oryctolagus cuniculus 180-184 10810281-0 2000 In vivo effect of endothelin-1 on plasma calcium and parathyroid hormone concentrations. Calcium 41-48 endothelin-1 Oryctolagus cuniculus 18-30 10810281-2 2000 In the present experiment, ET-1 was infused into rabbits to study the in vivo effect of ET-1 on the changes in calcium, magnesium, PTH and calcitonin concentrations. Calcium 111-118 endothelin-1 Oryctolagus cuniculus 88-92 10810281-4 2000 Infusion of ET-1 (1, 5, 10 and 20 ng/kg per min) induced a dose-dependent decline in plasma ionized calcium concentrations from 6.68+/-0.26 to 5.50+/-0.46 mg/dl (P<0.05) and a decrease in calcitonin concentrations from 48.6+/-6.5 to 32.5+/-4.7 pg/ml. Calcium 100-107 endothelin-1 Oryctolagus cuniculus 12-33 10810281-7 2000 In normal calcium concentration, ET-1 infusion gradually decreased PTH concentrations from 71.4+/-8.6 to 38.0+/-6.2 pg/ml. Calcium 10-17 endothelin-1 Oryctolagus cuniculus 33-37 10885493-2 2000 We hypothesized that HFO leads to decreased endothelin 1 (ET-1) and endothelin 3 (ET-3) release when compared to conventional mechanical ventilation (CMV) in lung-lavaged rabbits. 1,3,3,3-tetrafluoropropene 21-24 endothelin-1 Oryctolagus cuniculus 44-56 10885493-2 2000 We hypothesized that HFO leads to decreased endothelin 1 (ET-1) and endothelin 3 (ET-3) release when compared to conventional mechanical ventilation (CMV) in lung-lavaged rabbits. 1,3,3,3-tetrafluoropropene 21-24 endothelin-1 Oryctolagus cuniculus 58-62 10766139-5 2000 CONCLUSION: Administration of endothelin-1 to the microvasculature of the optic nerve leads to elevation of glutamate, aspartate, and glycine concentrations in the vitreous. Glutamic Acid 108-117 endothelin-1 Oryctolagus cuniculus 30-42 10779465-6 2000 Our hypothesis was that chronic NEP-I, in association with augmented cGMP, would inhibit ECE-1 conversion of big ET-1 to active ET-1, thus reducing tissue ET-1 concentrations and associated atheroma formation. Cyclic GMP 69-73 endothelin-1 Oryctolagus cuniculus 113-117 10779465-6 2000 Our hypothesis was that chronic NEP-I, in association with augmented cGMP, would inhibit ECE-1 conversion of big ET-1 to active ET-1, thus reducing tissue ET-1 concentrations and associated atheroma formation. Cyclic GMP 69-73 endothelin-1 Oryctolagus cuniculus 128-132 10779465-6 2000 Our hypothesis was that chronic NEP-I, in association with augmented cGMP, would inhibit ECE-1 conversion of big ET-1 to active ET-1, thus reducing tissue ET-1 concentrations and associated atheroma formation. Cyclic GMP 69-73 endothelin-1 Oryctolagus cuniculus 128-132 10766139-5 2000 CONCLUSION: Administration of endothelin-1 to the microvasculature of the optic nerve leads to elevation of glutamate, aspartate, and glycine concentrations in the vitreous. Aspartic Acid 119-128 endothelin-1 Oryctolagus cuniculus 30-42 10766139-5 2000 CONCLUSION: Administration of endothelin-1 to the microvasculature of the optic nerve leads to elevation of glutamate, aspartate, and glycine concentrations in the vitreous. Glycine 134-141 endothelin-1 Oryctolagus cuniculus 30-42 10825681-0 2000 Effects of endothelin-1 on KCl-induced contractions of airway smooth muscles. Potassium Chloride 27-30 endothelin-1 Oryctolagus cuniculus 11-23 10825681-1 2000 The objective of the study was to investigate the effect of endothelin-1 (ET-1) on KCl-induced contractions. Potassium Chloride 83-86 endothelin-1 Oryctolagus cuniculus 60-72 10825681-1 2000 The objective of the study was to investigate the effect of endothelin-1 (ET-1) on KCl-induced contractions. Potassium Chloride 83-86 endothelin-1 Oryctolagus cuniculus 74-78 10825681-5 2000 Methylene blue (10(-5) M) decreased all the contractions induced from simultaneous use of KCl and ET-1. Methylene Blue 0-14 endothelin-1 Oryctolagus cuniculus 98-102 10825681-6 2000 Methylene blue caused about 18% relaxation of contraction induced from KCl (80 mM) and ET-1 (10(-8) M). Methylene Blue 0-14 endothelin-1 Oryctolagus cuniculus 87-91 10825681-7 2000 Consequently ET-1 increases the KCl-induced contractions of airway smooth muscles. Potassium Chloride 32-35 endothelin-1 Oryctolagus cuniculus 13-17 10672851-0 2000 The protein kinase inhibitor fasudil protects against ischemic myocardial injury induced by endothelin-1 in the rabbit. fasudil 29-36 endothelin-1 Oryctolagus cuniculus 92-104 10720586-7 2000 Indomethacin (10 mg/kg) sharply potentiated the pressor response to ET-1 (1 nmol/kg), but not big ET-1, at all time points. Indomethacin 0-12 endothelin-1 Oryctolagus cuniculus 68-72 10720586-8 2000 In control animals, ET-1, but not big ET-1, also triggered an indomethacin-sensitive increase in circulating prostacyclin. Indomethacin 62-74 endothelin-1 Oryctolagus cuniculus 20-24 10720586-8 2000 In control animals, ET-1, but not big ET-1, also triggered an indomethacin-sensitive increase in circulating prostacyclin. Epoprostenol 109-121 endothelin-1 Oryctolagus cuniculus 20-24 10720586-9 2000 Finally, systemically administered big ET-1, but not big ET-2, induced a phosphoramidon-sensitive increase in plasma IR-ET. phosphoramidon 73-87 endothelin-1 Oryctolagus cuniculus 39-43 10655198-7 2000 Preincubation with 10 microM BQ123 (an ET(A) receptor antagonist) obviated inhibition by ET-1 (n=6). cyclo(Trp-Asp-Pro-Val-Leu) 29-34 endothelin-1 Oryctolagus cuniculus 89-93 10617910-0 2000 Improving effects of topical administration of iganidipine, a new calcium channel blocker, on the impaired visual evoked potential after endothelin-1 injection into the vitreous body of rabbits. iganidipine 47-58 endothelin-1 Oryctolagus cuniculus 137-149 10617910-1 2000 PURPOSE: To investigate the effect of topical iganidipine, a new dihydropyridine derivative calcium channel blocker, on impairment of the ocular circulation caused by endothelin-1 (ET-1), we examined modification of the effect of ET-1 on visual-evoked potential (VEP) in albino rabbits. iganidipine 46-57 endothelin-1 Oryctolagus cuniculus 167-179 10617910-1 2000 PURPOSE: To investigate the effect of topical iganidipine, a new dihydropyridine derivative calcium channel blocker, on impairment of the ocular circulation caused by endothelin-1 (ET-1), we examined modification of the effect of ET-1 on visual-evoked potential (VEP) in albino rabbits. iganidipine 46-57 endothelin-1 Oryctolagus cuniculus 181-185 10617910-3 2000 Then modification of the effect of ET-1 on the VEP by iganidipine was examined. iganidipine 54-65 endothelin-1 Oryctolagus cuniculus 35-39 10617910-4 2000 One hour after the topical instillation of 0.1% iganidipine (20 microl) or its vehicle, 10 pmol of ET-1 was injected into the vitreous in rabbits. iganidipine 48-59 endothelin-1 Oryctolagus cuniculus 99-103 10617910-7 2000 Topical administration of 0.1% iganidipine significantly suppressed the reduction of VEP amplitude for the entire 2-hour monitoring period after intravitreal injection of 10 pmol ET-1. iganidipine 31-42 endothelin-1 Oryctolagus cuniculus 179-183 10617910-9 2000 CONCLUSIONS: Iganidipine eyedrops may be useful for the treatment of ischemic retinal and optic nerve disorders related to abnormal ET-1 production for the maintenance of visual function. iganidipine 13-24 endothelin-1 Oryctolagus cuniculus 132-136 10679512-4 2000 Both the pressure-induced increase in ppET-1 and ET(B)-R expression required RNA synthesis because they were abolished by actinomycin D. Dactinomycin 122-135 endothelin-1 Oryctolagus cuniculus 38-44 10679512-6 2000 Thus, the pressure-induced expression of ppET-1 and activation of CCAAT-enhancer binding proteins beta and delta were blocked by the tyrosine kinase inhibitor herbimycin A, whereas ET(B)-R expression and the nuclear translocation of activator protein-1 were abolished by the protein kinase C inhibitor Ro 31-8220. herbimycin 159-171 endothelin-1 Oryctolagus cuniculus 41-47 10672851-4 2000 Here we demonstrate that ET-1 induces histologic and pathologic dysfunction in the rabbit myocardium and that such pathologic events are prevented by the Rho-kinase inhibitor fasudil. fasudil 175-182 endothelin-1 Oryctolagus cuniculus 25-29 10601179-6 1999 ET-1 induced an immediate PAP increase (DeltaPAP 4.3 +/- 0.4 mmHg at 10 min) that was attenuated by pretreatment with BQ-123 (P < 0.05 at 10 min and P < 0.01 thereafter) and that was more pronounced after BQ-788 (P < 0.01 at 10 min and P < 0.001 thereafter). cyclo(Trp-Asp-Pro-Val-Leu) 118-124 endothelin-1 Oryctolagus cuniculus 0-4 10657432-4 2000 METHODS: PD-98059, damnacanthal, wortmannin, AG-490, genistein, calphostin C, and staurosporine were used to inhibit, or relax, the ET-1-induced contraction of basilar artery, studied with an isometric tension system. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 9-17 endothelin-1 Oryctolagus cuniculus 132-136 10657432-4 2000 METHODS: PD-98059, damnacanthal, wortmannin, AG-490, genistein, calphostin C, and staurosporine were used to inhibit, or relax, the ET-1-induced contraction of basilar artery, studied with an isometric tension system. alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide 45-51 endothelin-1 Oryctolagus cuniculus 132-136 10657432-4 2000 METHODS: PD-98059, damnacanthal, wortmannin, AG-490, genistein, calphostin C, and staurosporine were used to inhibit, or relax, the ET-1-induced contraction of basilar artery, studied with an isometric tension system. Genistein 53-62 endothelin-1 Oryctolagus cuniculus 132-136 10657432-4 2000 METHODS: PD-98059, damnacanthal, wortmannin, AG-490, genistein, calphostin C, and staurosporine were used to inhibit, or relax, the ET-1-induced contraction of basilar artery, studied with an isometric tension system. calphostin C 64-76 endothelin-1 Oryctolagus cuniculus 132-136 10657432-4 2000 METHODS: PD-98059, damnacanthal, wortmannin, AG-490, genistein, calphostin C, and staurosporine were used to inhibit, or relax, the ET-1-induced contraction of basilar artery, studied with an isometric tension system. Staurosporine 82-95 endothelin-1 Oryctolagus cuniculus 132-136 10657432-7 2000 (2) MAPK inhibitors PD-98059 and U-0126 produced dose-dependent inhibition of ET-1-induced contraction. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 20-28 endothelin-1 Oryctolagus cuniculus 78-82 10657432-7 2000 (2) MAPK inhibitors PD-98059 and U-0126 produced dose-dependent inhibition of ET-1-induced contraction. U 0126 33-39 endothelin-1 Oryctolagus cuniculus 78-82 10657432-8 2000 (3) The Src tyrosine kinase inhibitor damnacanthal, the phosphatidylinositol-3 kinase inhibitor wortmannin, and the Janus tyrosine kinase(2) inhibitor AG-490 abolished ET-1-induced contraction. damnacanthal 38-50 endothelin-1 Oryctolagus cuniculus 168-172 10657432-8 2000 (3) The Src tyrosine kinase inhibitor damnacanthal, the phosphatidylinositol-3 kinase inhibitor wortmannin, and the Janus tyrosine kinase(2) inhibitor AG-490 abolished ET-1-induced contraction. alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide 151-157 endothelin-1 Oryctolagus cuniculus 168-172 10657432-9 2000 (4) The PKC inhibitor staurosporine but not calphostin C abolished ET-1-induced contraction, and the PTK inhibitor genistein partially reduced ET-1-induced contraction. Staurosporine 22-35 endothelin-1 Oryctolagus cuniculus 67-71 10657432-9 2000 (4) The PKC inhibitor staurosporine but not calphostin C abolished ET-1-induced contraction, and the PTK inhibitor genistein partially reduced ET-1-induced contraction. Genistein 115-124 endothelin-1 Oryctolagus cuniculus 143-147 10657432-10 2000 (5) In arteries precontracted by ET-1, PD-98059, U-0126, wortmannin, AG-490, genistein, and staurosporine produced concentration-dependent relaxation. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 39-47 endothelin-1 Oryctolagus cuniculus 33-37 10657432-10 2000 (5) In arteries precontracted by ET-1, PD-98059, U-0126, wortmannin, AG-490, genistein, and staurosporine produced concentration-dependent relaxation. U 0126 49-55 endothelin-1 Oryctolagus cuniculus 33-37 10657432-10 2000 (5) In arteries precontracted by ET-1, PD-98059, U-0126, wortmannin, AG-490, genistein, and staurosporine produced concentration-dependent relaxation. Wortmannin 57-67 endothelin-1 Oryctolagus cuniculus 33-37 10657432-10 2000 (5) In arteries precontracted by ET-1, PD-98059, U-0126, wortmannin, AG-490, genistein, and staurosporine produced concentration-dependent relaxation. alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide 69-75 endothelin-1 Oryctolagus cuniculus 33-37 10657432-10 2000 (5) In arteries precontracted by ET-1, PD-98059, U-0126, wortmannin, AG-490, genistein, and staurosporine produced concentration-dependent relaxation. Genistein 77-86 endothelin-1 Oryctolagus cuniculus 33-37 10657432-10 2000 (5) In arteries precontracted by ET-1, PD-98059, U-0126, wortmannin, AG-490, genistein, and staurosporine produced concentration-dependent relaxation. Staurosporine 92-105 endothelin-1 Oryctolagus cuniculus 33-37 10657432-12 2000 (7) PD-98059, U-0126, genistein, AG-490, and damnacanthal, but not staurosporine or wortmannin, abolished the effect of ET-1 on MAPK immunoreactivity. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 4-12 endothelin-1 Oryctolagus cuniculus 120-124 10657432-12 2000 (7) PD-98059, U-0126, genistein, AG-490, and damnacanthal, but not staurosporine or wortmannin, abolished the effect of ET-1 on MAPK immunoreactivity. U 0126 14-20 endothelin-1 Oryctolagus cuniculus 120-124 10657432-12 2000 (7) PD-98059, U-0126, genistein, AG-490, and damnacanthal, but not staurosporine or wortmannin, abolished the effect of ET-1 on MAPK immunoreactivity. Genistein 22-31 endothelin-1 Oryctolagus cuniculus 120-124 10650177-5 2000 In contrast, plasma endothelin-1 levels increased only in the 1.5% cholesterol diet group (2.7 times). Cholesterol 67-78 endothelin-1 Oryctolagus cuniculus 20-32 11097113-5 2000 A decoy oligodeoxynucleotide directed against AP-1 inhibited deformation-induced ppET-1 expression in the rabbit jugular vein as well as in porcine aorta EC and human umbilical vein EC but not in the rabbit carotid artery. Oligodeoxyribonucleotides 8-28 endothelin-1 Oryctolagus cuniculus 81-87 11097113-7 2000 Moreover, a C/EBP-specific decoy oligodeoxynucleotide abolished ppET-1 expression in the endothelium of the rabbit carotid artery, but not in the jugular vein where basal ET-1 synthesis was greatly enhanced instead. Oligodeoxyribonucleotides 33-53 endothelin-1 Oryctolagus cuniculus 64-70 11097113-7 2000 Moreover, a C/EBP-specific decoy oligodeoxynucleotide abolished ppET-1 expression in the endothelium of the rabbit carotid artery, but not in the jugular vein where basal ET-1 synthesis was greatly enhanced instead. Oligodeoxyribonucleotides 33-53 endothelin-1 Oryctolagus cuniculus 66-70 10883463-0 2000 Heparin reduces serum levels of endothelin-1 and hepatic ischemia reperfusion injury in rabbits. Heparin 0-7 endothelin-1 Oryctolagus cuniculus 32-44 10883463-2 2000 Heparin was injected into rabbits after inducing partial hepatic ischemia for 1 h. Thereafter, the serum levels of endothelin-1 (ET-1) and liver transaminase, and tissue levels of oxidized and deoxidized hemoglobin (oxHb, deoxHb) in the reperfused liver were analyzed. Heparin 0-7 endothelin-1 Oryctolagus cuniculus 115-127 10883463-4 2000 The increased serum levels of ET-1 and liver transaminase after reperfusion were significantly reduced by heparin (P > 0.01). Heparin 106-113 endothelin-1 Oryctolagus cuniculus 30-34 10883463-8 2000 Microscopically, heparin appeared to normalize I/R-induced activation of hepatic stellate cells which are the target cells for ET-1. Heparin 17-24 endothelin-1 Oryctolagus cuniculus 127-131 10883463-9 2000 These results suggest that heparin improves the hepatic I/R injury caused by sinusoidal microscirculatory disturbances partly via an inhibition of the ET-1 increase. Heparin 27-34 endothelin-1 Oryctolagus cuniculus 151-155 10619182-2 1999 ET-1 (10(-8) M) had a biphasic effect on I(Ca) (direct effect), causing a transient decrease that was followed by a long-lasting increase which is much smaller than the increase induced by isoprenaline (ISO). Isoproterenol 189-201 endothelin-1 Oryctolagus cuniculus 0-4 10619182-2 1999 ET-1 (10(-8) M) had a biphasic effect on I(Ca) (direct effect), causing a transient decrease that was followed by a long-lasting increase which is much smaller than the increase induced by isoprenaline (ISO). Isoproterenol 203-206 endothelin-1 Oryctolagus cuniculus 0-4 10619182-3 1999 The effect of ET-1 on I(Ca) was abolished by a selective ET(A) receptor antagonist, FR139317 (10(-6) M). FR 139317 84-92 endothelin-1 Oryctolagus cuniculus 14-18 10619182-4 1999 The increase in I(Ca) induced by ET-1 (10(-8) M) was enhanced by a selective ET(B) receptor antagonist, BQ-788 (10(-6) M), as the transient decrease but not the increase in I(Ca) induced by ET-1 (10(-8) M) was suppressed by BQ-788. BQ 788 104-110 endothelin-1 Oryctolagus cuniculus 33-37 10619182-4 1999 The increase in I(Ca) induced by ET-1 (10(-8) M) was enhanced by a selective ET(B) receptor antagonist, BQ-788 (10(-6) M), as the transient decrease but not the increase in I(Ca) induced by ET-1 (10(-8) M) was suppressed by BQ-788. BQ 788 104-110 endothelin-1 Oryctolagus cuniculus 190-194 10619182-4 1999 The increase in I(Ca) induced by ET-1 (10(-8) M) was enhanced by a selective ET(B) receptor antagonist, BQ-788 (10(-6) M), as the transient decrease but not the increase in I(Ca) induced by ET-1 (10(-8) M) was suppressed by BQ-788. BQ 788 224-230 endothelin-1 Oryctolagus cuniculus 33-37 10619182-5 1999 In the presence of ISO (10(-6) M), ET-1 elicited a more pronounced inhibitory effect: at 10(-9)-10(-7) M ET-1 inhibited the ISO-induced increase in I(Ca) in a concentration-dependent manner (anti-adrenergic effect). Isoproterenol 19-22 endothelin-1 Oryctolagus cuniculus 35-39 10619182-5 1999 In the presence of ISO (10(-6) M), ET-1 elicited a more pronounced inhibitory effect: at 10(-9)-10(-7) M ET-1 inhibited the ISO-induced increase in I(Ca) in a concentration-dependent manner (anti-adrenergic effect). Isoproterenol 19-22 endothelin-1 Oryctolagus cuniculus 105-109 10619182-8 1999 The increases in I(Ca) induced by forskolin (10(-6) M), 3-isobutyl-1-methylxanthine (10(-4) M), and 8-bromo-cyclic AMP (3x10(-4) M) were also suppressed by ET-1 (10(-8) M). 1-Methyl-3-isobutylxanthine 56-83 endothelin-1 Oryctolagus cuniculus 156-160 10619182-9 1999 In summary, ET-1 has a differential effect on I(Ca) in the absence and in the presence of ISO: ET- I has a feeble biphasic action on the baseline I(Ca) and, in addition, it elicits a pronounced anti-adrenergic effect on the ISO-induced increase in I(Ca). 5,8,11-eicosatriynoic acid 95-100 endothelin-1 Oryctolagus cuniculus 12-16 10619182-10 1999 Pertussis toxin-sensitive G protein is responsible for the anti-adrenergic effect of ET-1 on I(Ca), but the anti-adrenergic effect of ET-1 may involve also the regulation at the level of signaling process beyond the cyclic AMP generation. Cyclic AMP 216-226 endothelin-1 Oryctolagus cuniculus 134-138 10619183-3 1999 Our previous study showed that endothelin-1 induced bronchial hyperresponsiveness to inhaled histamine in the rabbit. Histamine 93-102 endothelin-1 Oryctolagus cuniculus 31-43 10619183-6 1999 Moreover, bosentan (from 2.5 mg kg(-1) to 10 mg kg(-1)), an ET(A)/ET(B) receptor antagonist, also inhibited the bronchial hyperresponsiveness achieved 24 h following endothelin-1 challenge (P<0.01), but with no difference from FR 139317. Bosentan 10-18 endothelin-1 Oryctolagus cuniculus 166-178 10548680-9 1999 This effect was reversed by the endothelin-1 (ET-1) antagonist BQ-123 (1 micromol/L). cyclo(Trp-Asp-Pro-Val-Leu) 63-69 endothelin-1 Oryctolagus cuniculus 32-44 10548680-9 1999 This effect was reversed by the endothelin-1 (ET-1) antagonist BQ-123 (1 micromol/L). cyclo(Trp-Asp-Pro-Val-Leu) 63-69 endothelin-1 Oryctolagus cuniculus 46-50 10504277-5 1999 The injection of ET-1 increased aqueous prostaglandin E(2) concentration, which was inhibited by pre-treatment with ET(A) receptor antagonist. Dinoprostone 40-58 endothelin-1 Oryctolagus cuniculus 17-21 10504277-7 1999 In conclusion, ET(A) receptor mediates the increases in aqueous protein and prostaglandin E(2) concentration induced by ET-1 injection, and this inflammatory reaction occurs via the cyclooxygenase pathway of arachidonic acid cascade. Dinoprostone 76-94 endothelin-1 Oryctolagus cuniculus 120-124 10504277-7 1999 In conclusion, ET(A) receptor mediates the increases in aqueous protein and prostaglandin E(2) concentration induced by ET-1 injection, and this inflammatory reaction occurs via the cyclooxygenase pathway of arachidonic acid cascade. Arachidonic Acid 208-224 endothelin-1 Oryctolagus cuniculus 120-124 10731793-7 2000 RESULTS: ET-1 induced an increase of AT (147 +/- 33%), dT/dtmax (154 +/- 39%) and dT/dtmin (145 +/- 38%), while the duration of the twitch did not vary significantly. Thymidine 55-57 endothelin-1 Oryctolagus cuniculus 9-13 10731793-7 2000 RESULTS: ET-1 induced an increase of AT (147 +/- 33%), dT/dtmax (154 +/- 39%) and dT/dtmin (145 +/- 38%), while the duration of the twitch did not vary significantly. dtmax 58-63 endothelin-1 Oryctolagus cuniculus 9-13 10731793-7 2000 RESULTS: ET-1 induced an increase of AT (147 +/- 33%), dT/dtmax (154 +/- 39%) and dT/dtmin (145 +/- 38%), while the duration of the twitch did not vary significantly. Thymidine 82-84 endothelin-1 Oryctolagus cuniculus 9-13 10731793-7 2000 RESULTS: ET-1 induced an increase of AT (147 +/- 33%), dT/dtmax (154 +/- 39%) and dT/dtmin (145 +/- 38%), while the duration of the twitch did not vary significantly. dtmin 85-90 endothelin-1 Oryctolagus cuniculus 9-13 10731793-9 2000 CONCLUSIONS: This study showed that, in addition to the well-known positive inotropic effect, ET-1 improves diastolic function by accelerating relaxation rate (dT/dtmin) and decreasing RT. Thymidine 160-162 endothelin-1 Oryctolagus cuniculus 94-98 10731793-9 2000 CONCLUSIONS: This study showed that, in addition to the well-known positive inotropic effect, ET-1 improves diastolic function by accelerating relaxation rate (dT/dtmin) and decreasing RT. dtmin 163-168 endothelin-1 Oryctolagus cuniculus 94-98 10562595-4 1999 In arteries preconstricted with endothelin-1 to a similar tension level, MgSO(4) caused greater relaxation of juvenile rabbit PA than that of the newborn rabbit PA. Verapamil, a voltage-dependent Ca(2+) channel blocker, attenuated magnesium-induced relaxation in juvenile rabbit PA but not in newborn PA. Magnesium Sulfate 73-80 endothelin-1 Oryctolagus cuniculus 32-44 10533980-0 1999 Endothelin-1 effect on tyrosine phosphorylation and on tyrosine phosphatase (PTP-1C) translocation in rabbit platelets. Tyrosine 23-31 endothelin-1 Oryctolagus cuniculus 0-12 10533980-1 1999 This study examined the temporal relationships of endothelin-1-stimulated rabbit platelets tyrosine phosphorylated proteins. Tyrosine 91-99 endothelin-1 Oryctolagus cuniculus 50-62 10533980-2 1999 The effect of endothelin-1 on tyrosine phosphorylation was dose- and time-dependent and caused a rapid tyrosine phosphorylation of three groups of proteins in the molecular mass range 70-100 kDa, 100-150 kDa and 150-200 kDa. Tyrosine 30-38 endothelin-1 Oryctolagus cuniculus 14-26 10533980-2 1999 The effect of endothelin-1 on tyrosine phosphorylation was dose- and time-dependent and caused a rapid tyrosine phosphorylation of three groups of proteins in the molecular mass range 70-100 kDa, 100-150 kDa and 150-200 kDa. Tyrosine 103-111 endothelin-1 Oryctolagus cuniculus 14-26 10629852-0 1999 [Role of L-arginine--endogenous NOS inhibitors--endothelin-1 pathway for the vascular remodelling]. Arginine 9-19 endothelin-1 Oryctolagus cuniculus 48-60 10629852-2 1999 We describe herein the role of L-arginine-endogenous NOS inhibitors-endothelin-1 pathway for the vascular remodelling after endothelial denudation of the rabbit carotid artery. Arginine 31-41 endothelin-1 Oryctolagus cuniculus 68-80 10629852-4 1999 An accumulation of endogenous inhibitors (L-NMMA and ADMA) in regenerated endothelial cells after the endothelial denudation was accompanied by the decreased NO generation, the increased endothelin-1 content within the vessel wall and the occurrence of intimal hyperplasia. omega-N-Methylarginine 42-48 endothelin-1 Oryctolagus cuniculus 187-199 10629852-4 1999 An accumulation of endogenous inhibitors (L-NMMA and ADMA) in regenerated endothelial cells after the endothelial denudation was accompanied by the decreased NO generation, the increased endothelin-1 content within the vessel wall and the occurrence of intimal hyperplasia. N,N-dimethylarginine 53-57 endothelin-1 Oryctolagus cuniculus 187-199 10629852-5 1999 Endothelin-1 content within the vessel wall was significantly increased after the exogenous L-NMMA administration for 2 weeks, suggesting that accumulated L-NMMA results in the decreased NO generation and, in turn, increases endothelin-1 content. omega-N-Methylarginine 92-98 endothelin-1 Oryctolagus cuniculus 0-12 10629852-5 1999 Endothelin-1 content within the vessel wall was significantly increased after the exogenous L-NMMA administration for 2 weeks, suggesting that accumulated L-NMMA results in the decreased NO generation and, in turn, increases endothelin-1 content. omega-N-Methylarginine 92-98 endothelin-1 Oryctolagus cuniculus 225-237 10629852-5 1999 Endothelin-1 content within the vessel wall was significantly increased after the exogenous L-NMMA administration for 2 weeks, suggesting that accumulated L-NMMA results in the decreased NO generation and, in turn, increases endothelin-1 content. omega-N-Methylarginine 155-161 endothelin-1 Oryctolagus cuniculus 0-12 10629852-5 1999 Endothelin-1 content within the vessel wall was significantly increased after the exogenous L-NMMA administration for 2 weeks, suggesting that accumulated L-NMMA results in the decreased NO generation and, in turn, increases endothelin-1 content. omega-N-Methylarginine 155-161 endothelin-1 Oryctolagus cuniculus 225-237 10629852-6 1999 Endothelin-1 facilitated the [3H]-L-NMMA uptake by endothelial cell and brought about the potentiation of L-NMMA-mediated inhibition of NO generation. Tritium 30-32 endothelin-1 Oryctolagus cuniculus 0-12 10629852-6 1999 Endothelin-1 facilitated the [3H]-L-NMMA uptake by endothelial cell and brought about the potentiation of L-NMMA-mediated inhibition of NO generation. omega-N-Methylarginine 34-40 endothelin-1 Oryctolagus cuniculus 0-12 10629852-6 1999 Endothelin-1 facilitated the [3H]-L-NMMA uptake by endothelial cell and brought about the potentiation of L-NMMA-mediated inhibition of NO generation. omega-N-Methylarginine 106-112 endothelin-1 Oryctolagus cuniculus 0-12 10629852-7 1999 These results strongly suggest that the L-arginine--endogenous NOS inhibitors--endothelin-1 pathway plays an active role for vascular remodelling. Arginine 40-50 endothelin-1 Oryctolagus cuniculus 79-91 10548119-11 1999 The CA/DA ratio was significantly increased relative to baseline in the ET-1 treated eyes. da 7-9 endothelin-1 Oryctolagus cuniculus 72-76 10480479-2 1999 Under baseline conditions, the ET(A) receptor antagonist BQ-123 did not change basal renal hemodynamics, but completely prevented endothelin-1 (ET-1)-induced renal vasoconstriction. cyclo(Trp-Asp-Pro-Val-Leu) 57-63 endothelin-1 Oryctolagus cuniculus 130-142 10480479-2 1999 Under baseline conditions, the ET(A) receptor antagonist BQ-123 did not change basal renal hemodynamics, but completely prevented endothelin-1 (ET-1)-induced renal vasoconstriction. cyclo(Trp-Asp-Pro-Val-Leu) 57-63 endothelin-1 Oryctolagus cuniculus 144-148 10601179-6 1999 ET-1 induced an immediate PAP increase (DeltaPAP 4.3 +/- 0.4 mmHg at 10 min) that was attenuated by pretreatment with BQ-123 (P < 0.05 at 10 min and P < 0.01 thereafter) and that was more pronounced after BQ-788 (P < 0.01 at 10 min and P < 0.001 thereafter). BQ 788 211-217 endothelin-1 Oryctolagus cuniculus 0-4 10377083-1 1999 BACKGROUND: We have previously shown that nitroglycerin (NTG) therapy increases vascular expression of endothelin 1 (ET-1) and stimulates vascular superoxide (O2.-) production via activation of NADH/NADPH oxidases. Nitroglycerin 42-55 endothelin-1 Oryctolagus cuniculus 103-115 10488689-5 1999 As shown in the rabbit basilar artery contracted by endothelin-1 KRN2391 elicited a concentration-dependent relaxation. N-cyano-N'-(2-nitroxyethyl)-3-pyridinecarboximidamide methanesulfonate 65-72 endothelin-1 Oryctolagus cuniculus 52-64 10377083-1 1999 BACKGROUND: We have previously shown that nitroglycerin (NTG) therapy increases vascular expression of endothelin 1 (ET-1) and stimulates vascular superoxide (O2.-) production via activation of NADH/NADPH oxidases. Nitroglycerin 42-55 endothelin-1 Oryctolagus cuniculus 117-121 10377083-1 1999 BACKGROUND: We have previously shown that nitroglycerin (NTG) therapy increases vascular expression of endothelin 1 (ET-1) and stimulates vascular superoxide (O2.-) production via activation of NADH/NADPH oxidases. Nitroglycerin 57-60 endothelin-1 Oryctolagus cuniculus 103-115 10377083-1 1999 BACKGROUND: We have previously shown that nitroglycerin (NTG) therapy increases vascular expression of endothelin 1 (ET-1) and stimulates vascular superoxide (O2.-) production via activation of NADH/NADPH oxidases. Nitroglycerin 57-60 endothelin-1 Oryctolagus cuniculus 117-121 10377083-3 1999 We hypothesized that either angiotensin II or ET-1 may increase vascular O2.- production during nitrate therapy. Superoxides 73-75 endothelin-1 Oryctolagus cuniculus 46-50 10377083-3 1999 We hypothesized that either angiotensin II or ET-1 may increase vascular O2.- production during nitrate therapy. Nitrates 96-103 endothelin-1 Oryctolagus cuniculus 46-50 10377083-14 1999 CONCLUSIONS: The positive effects of AT1 and ET-1 receptor blockade on tolerance and O2.- production imply a pathophysiological role for angiotensin II and to some extent for ET-1 in the development of nitrate tolerance. Superoxides 85-87 endothelin-1 Oryctolagus cuniculus 45-49 10377083-14 1999 CONCLUSIONS: The positive effects of AT1 and ET-1 receptor blockade on tolerance and O2.- production imply a pathophysiological role for angiotensin II and to some extent for ET-1 in the development of nitrate tolerance. Superoxides 85-87 endothelin-1 Oryctolagus cuniculus 175-179 10377083-14 1999 CONCLUSIONS: The positive effects of AT1 and ET-1 receptor blockade on tolerance and O2.- production imply a pathophysiological role for angiotensin II and to some extent for ET-1 in the development of nitrate tolerance. Nitrates 202-209 endothelin-1 Oryctolagus cuniculus 45-49 10377083-14 1999 CONCLUSIONS: The positive effects of AT1 and ET-1 receptor blockade on tolerance and O2.- production imply a pathophysiological role for angiotensin II and to some extent for ET-1 in the development of nitrate tolerance. Nitrates 202-209 endothelin-1 Oryctolagus cuniculus 175-179 10678145-0 1999 High glucose enhances mitogenic response to endothelin-1 in rabbit vascular smooth muscle cells. Glucose 5-12 endothelin-1 Oryctolagus cuniculus 44-56 10678145-1 1999 AIM: To examine the effects of high glucose on the mitogenic response of rabbit aortic vascular smooth muscle cells (VSMC) to endothelin-1 (ET-1). Glucose 36-43 endothelin-1 Oryctolagus cuniculus 126-138 10678145-5 1999 RESULTS: At a concentration range from 10(-12) to 10(-8) mol.L-1, ET-1 stimulated [3H]thymidine incorporation and phospho-p44/42 MAPK expression in VSMC in a concentration-dependent manner. Tritium 83-85 endothelin-1 Oryctolagus cuniculus 66-70 10678145-1 1999 AIM: To examine the effects of high glucose on the mitogenic response of rabbit aortic vascular smooth muscle cells (VSMC) to endothelin-1 (ET-1). Glucose 36-43 endothelin-1 Oryctolagus cuniculus 140-144 10678145-6 1999 From 10(-11) to 10(-8) mol.L-1, the mitogenic effect of ET-1 was higher in VSMC cultured in high glucose at equivalent concentration than cells cultured in normal glucose or high osmolality (P < 0.05 or P < 0.01), but no marked difference was observed in the growth response between cells cultured under the latter two conditions. Glucose 97-104 endothelin-1 Oryctolagus cuniculus 56-60 10678145-6 1999 From 10(-11) to 10(-8) mol.L-1, the mitogenic effect of ET-1 was higher in VSMC cultured in high glucose at equivalent concentration than cells cultured in normal glucose or high osmolality (P < 0.05 or P < 0.01), but no marked difference was observed in the growth response between cells cultured under the latter two conditions. Glucose 163-170 endothelin-1 Oryctolagus cuniculus 56-60 10678145-7 1999 Similarly, ET-1 increased expression of phospho-p44/42 MAPK by 60%-65% in VSMC cultured in high glucose, compared with cells in normal glucose or high osmolality. vsmc 74-78 endothelin-1 Oryctolagus cuniculus 11-15 10678145-7 1999 Similarly, ET-1 increased expression of phospho-p44/42 MAPK by 60%-65% in VSMC cultured in high glucose, compared with cells in normal glucose or high osmolality. Glucose 96-103 endothelin-1 Oryctolagus cuniculus 11-15 10678145-7 1999 Similarly, ET-1 increased expression of phospho-p44/42 MAPK by 60%-65% in VSMC cultured in high glucose, compared with cells in normal glucose or high osmolality. Glucose 135-142 endothelin-1 Oryctolagus cuniculus 11-15 10678145-8 1999 CONCLUSION: VSMC cultured in high glucose exhibited increased mitogenic response to ET-1, which seemed to be related to the enhanced expression of phospho-p44/42 MAPK. Glucose 34-41 endothelin-1 Oryctolagus cuniculus 84-88 10509735-9 1999 The alterations in BF, PGI2, ET-1, and NOx in response to AZ, suggest that the mechanism of AZ-induced vasodilatation may involve a cascade that is triggered by CO2 retention similar to that caused by the inhalation of CO2. Acetazolamide 92-94 endothelin-1 Oryctolagus cuniculus 29-33 10225166-6 1999 ET-1 infusion produced an increase in mean arterial pressure (83.9 +/- 3.9 mmHg vs. 50.1 +/- 4.1 mmHg at 120 min; P < 0.01) associated with higher serum lactate concentrations (12.6 +/- 1.3 vs. 5.4 +/- 0.3 mg dL-1; P < 0.001) and a delayed bacterial elimination from the blood compared with controls. Lactic Acid 156-163 endothelin-1 Oryctolagus cuniculus 0-4 9863646-12 1998 To investigate if endothelin-1 (ET-1) intervened in the endothelium-dependent contractile response to ACH in the presence of OXY-dependent tone, vessels were incubated in the presence of BQ123 (1 micromol l(-1)), an ETA receptor antagonist. Acetylcholine 102-105 endothelin-1 Oryctolagus cuniculus 18-30 10214485-0 1999 Does thromboxane A2 synthase inhibitor UK-38485 prevent the vasoconstrictor effects of endothelin-1 on rabbit basilar artery? dazmegrel 39-47 endothelin-1 Oryctolagus cuniculus 87-99 10214485-3 1999 The purpose of this study was to investigate the role of a novel thromboxane A2-synthase inhibitor UK 38485 on the acute vascular and morphological effects of Endothelin-1 applied intra-arterially on rabbit basilar arteries. dazmegrel 99-107 endothelin-1 Oryctolagus cuniculus 159-171 10214485-7 1999 The results indicated that there might have been an interaction between Endothelin-1 and the prostaglandin synthesis mechanism in acute cerebral vasoconstriction. Prostaglandins 93-106 endothelin-1 Oryctolagus cuniculus 72-84 9882755-13 1999 Thapsigargin (1 microM) completely depleted Ca2+ from caffeine- and ET1-sensitive sarcoplasmic reticulum but did not inhibit the ET1-induced sustained elevation of [Ca2+]i. Thapsigargin 0-12 endothelin-1 Oryctolagus cuniculus 68-71 9882755-14 1999 ET1 effects on [Ca2+]i were prevented by the ETA receptor antagonist BQ123 (cyclo-D-Asp-Pro-D-Val-Leu-D-Trp). cyclo(Trp-Asp-Pro-Val-Leu) 69-74 endothelin-1 Oryctolagus cuniculus 0-3 9882755-14 1999 ET1 effects on [Ca2+]i were prevented by the ETA receptor antagonist BQ123 (cyclo-D-Asp-Pro-D-Val-Leu-D-Trp). cyclo-d-asp-pro-d-val-leu-d-trp 76-107 endothelin-1 Oryctolagus cuniculus 0-3 10051126-4 1999 The response of endothelium-denuded RPA rings to endothelin-1 (ET-1, pD2 = 7.84 +/- 0.03) was only partially inhibited by BQ123 (10 microM), an ET(A)R antagonist. cyclo(Trp-Asp-Pro-Val-Leu) 122-127 endothelin-1 Oryctolagus cuniculus 49-61 10051126-4 1999 The response of endothelium-denuded RPA rings to endothelin-1 (ET-1, pD2 = 7.84 +/- 0.03) was only partially inhibited by BQ123 (10 microM), an ET(A)R antagonist. cyclo(Trp-Asp-Pro-Val-Leu) 122-127 endothelin-1 Oryctolagus cuniculus 63-67 10051126-8 1999 Pretreatment with S6c had little effect on the response to ET-1, but BQ123 (10 microM) caused a parallel shift to the right of the residual ETAR-mediated response to ET-1 (pD2 = 7.84 +/- 0.03 before S6c, 7.93 +/- 0.03 after S6c, 6.81 +/- 0.05 after BQ123). cyclo(Trp-Asp-Pro-Val-Leu) 69-74 endothelin-1 Oryctolagus cuniculus 166-170 9843544-8 1998 In arteries precontracted with ET-1 (10(-8)-3 x 10(-8) M), relaxation to sodium nitroprusside (10(-8)-10(-4) M) was increased at 41 and 44 degreesC vs. at 37 degreesC, but that of ACh (10(-8)-10(-4) M) or adenosine (10(-8)-10(-4) M) was not different at all temperatures studied. Nitroprusside 73-93 endothelin-1 Oryctolagus cuniculus 31-35 9843544-8 1998 In arteries precontracted with ET-1 (10(-8)-3 x 10(-8) M), relaxation to sodium nitroprusside (10(-8)-10(-4) M) was increased at 41 and 44 degreesC vs. at 37 degreesC, but that of ACh (10(-8)-10(-4) M) or adenosine (10(-8)-10(-4) M) was not different at all temperatures studied. Acetylcholine 180-183 endothelin-1 Oryctolagus cuniculus 31-35 9843544-8 1998 In arteries precontracted with ET-1 (10(-8)-3 x 10(-8) M), relaxation to sodium nitroprusside (10(-8)-10(-4) M) was increased at 41 and 44 degreesC vs. at 37 degreesC, but that of ACh (10(-8)-10(-4) M) or adenosine (10(-8)-10(-4) M) was not different at all temperatures studied. Adenosine 205-214 endothelin-1 Oryctolagus cuniculus 31-35 9886875-8 1998 RESULTS: In the infused kidney ET-1 (2 ng/kg/min) reduced renal blood flow (RBFprobe; 52+/-8%), cortical perfusion (37+/-7%), glomerular filtration rate (GFR; 49+/-8%), urine flow (47+/-14%) and sodium excretion (49+/-13%), but not medullary perfusion (5+/-6%). Sodium 195-201 endothelin-1 Oryctolagus cuniculus 31-35 9886875-10 1998 TAK-044 dose-dependently reversed the effects of ET-1 on RBFprobe and cortical perfusion. TAK 044 0-7 endothelin-1 Oryctolagus cuniculus 49-53 9886875-14 1998 TAK-044 antagonizes local renal vascular responses to ET-1. TAK 044 0-7 endothelin-1 Oryctolagus cuniculus 54-58 9863646-12 1998 To investigate if endothelin-1 (ET-1) intervened in the endothelium-dependent contractile response to ACH in the presence of OXY-dependent tone, vessels were incubated in the presence of BQ123 (1 micromol l(-1)), an ETA receptor antagonist. Acetylcholine 102-105 endothelin-1 Oryctolagus cuniculus 32-36 9863646-14 1998 These data indicate that activation of endothelial alpha2-AR triggers an endothelium-dependent, ET-1 mediated, contraction to ACH. Acetylcholine 126-129 endothelin-1 Oryctolagus cuniculus 96-100 9844139-10 1998 CsA and tacrolimus (0.5 to 5000 microgram/liter) induced a significant, dose-dependent increase of ET-1 and ET-3 release by PT-1 cells with a maximum stimulation at a CsA concentration of 500 microgram/liter (P < 0.001) and a tacrolimus concentration of 50 microgram/liter (P < 0.001). Cyclosporine 0-3 endothelin-1 Oryctolagus cuniculus 99-112 9824081-17 1998 The endothelin-1 effects are probably mediated via endothelin-A receptors since the endothelin-A receptor antagonist LU135252 was able to suppress the early pressure reaction after FMLP injection, whereas the cyclooxygenase inhibitor diclofenac was able to reduce the second pressure increase. darusentan 117-125 endothelin-1 Oryctolagus cuniculus 4-16 9824081-17 1998 The endothelin-1 effects are probably mediated via endothelin-A receptors since the endothelin-A receptor antagonist LU135252 was able to suppress the early pressure reaction after FMLP injection, whereas the cyclooxygenase inhibitor diclofenac was able to reduce the second pressure increase. Diclofenac 234-244 endothelin-1 Oryctolagus cuniculus 4-16 9844139-10 1998 CsA and tacrolimus (0.5 to 5000 microgram/liter) induced a significant, dose-dependent increase of ET-1 and ET-3 release by PT-1 cells with a maximum stimulation at a CsA concentration of 500 microgram/liter (P < 0.001) and a tacrolimus concentration of 50 microgram/liter (P < 0.001). Tacrolimus 8-18 endothelin-1 Oryctolagus cuniculus 99-112 9844139-10 1998 CsA and tacrolimus (0.5 to 5000 microgram/liter) induced a significant, dose-dependent increase of ET-1 and ET-3 release by PT-1 cells with a maximum stimulation at a CsA concentration of 500 microgram/liter (P < 0.001) and a tacrolimus concentration of 50 microgram/liter (P < 0.001). Cyclosporine 167-170 endothelin-1 Oryctolagus cuniculus 99-112 9844139-12 1998 Coincubation of PT-1 cells with CsA or tacrolimus and HGF or EGF also resulted in a marked reduction of ET-1 release. Cyclosporine 32-35 endothelin-1 Oryctolagus cuniculus 104-108 9844139-12 1998 Coincubation of PT-1 cells with CsA or tacrolimus and HGF or EGF also resulted in a marked reduction of ET-1 release. Tacrolimus 39-49 endothelin-1 Oryctolagus cuniculus 104-108 9844139-13 1998 CONCLUSIONS: The present data suggest that ET-1 and ET-3 release by cultured rabbit proximal tubule cells are regulated differently, and that the stimulatory effect of CsA and tacrolimus on ET-1 release is antagonized by HGF and EGF. Cyclosporine 168-171 endothelin-1 Oryctolagus cuniculus 43-56 9844139-13 1998 CONCLUSIONS: The present data suggest that ET-1 and ET-3 release by cultured rabbit proximal tubule cells are regulated differently, and that the stimulatory effect of CsA and tacrolimus on ET-1 release is antagonized by HGF and EGF. Tacrolimus 176-186 endothelin-1 Oryctolagus cuniculus 43-56 9844139-13 1998 CONCLUSIONS: The present data suggest that ET-1 and ET-3 release by cultured rabbit proximal tubule cells are regulated differently, and that the stimulatory effect of CsA and tacrolimus on ET-1 release is antagonized by HGF and EGF. Tacrolimus 176-186 endothelin-1 Oryctolagus cuniculus 43-47 10452081-5 1998 The administration of PGE1 showed an advantageous effect in prevention of thrombosis and muscle "progressive necrosis" through improvement in RCD, reduction of PAR and the content of ET-1 in local circulation. Alprostadil 22-26 endothelin-1 Oryctolagus cuniculus 183-187 9840425-4 1998 Our data show that ET-1 challenge to rabbits does not modify basal lung function but results in an increased airway responsiveness to inhaled histamine. Histamine 142-151 endothelin-1 Oryctolagus cuniculus 19-23 9840425-8 1998 While capsaicin had no significant effect on the acute bronchoconstriction induced by endothelin-1, this dose was sufficient to significantly inhibit the increase in airway responsiveness to inhaled histamine, achieved 24 h following endothelin-1 challenge. Histamine 199-208 endothelin-1 Oryctolagus cuniculus 234-246 9840425-9 1998 These results indicate that ET-1 may play a role in the development of bronchial hyperresponsiveness to inhaled histamine and that the maintenance of this state is unrelated to a detectable alteration in cellular infiltration within the airway lumen, but probably via the involvement of capsaicin-sensitive nerves. Histamine 112-121 endothelin-1 Oryctolagus cuniculus 28-32 9840425-9 1998 These results indicate that ET-1 may play a role in the development of bronchial hyperresponsiveness to inhaled histamine and that the maintenance of this state is unrelated to a detectable alteration in cellular infiltration within the airway lumen, but probably via the involvement of capsaicin-sensitive nerves. Capsaicin 287-296 endothelin-1 Oryctolagus cuniculus 28-32 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. PD 145065 364-372 endothelin-1 Oryctolagus cuniculus 123-135 9788775-4 1998 RES-701-1 was selective for the ET(B1) receptor, as the endothelin-1 constriction elicited in the presence of BQ610 (homopiperidenyl-CO-Leu-D-Trp (CHO)-D-Trp-OH), an endothelin ET(A) receptor antagonist, was enhanced by RES-701-1, and relaxed by BQ788. BQ 610 110-115 endothelin-1 Oryctolagus cuniculus 56-68 9788775-4 1998 RES-701-1 was selective for the ET(B1) receptor, as the endothelin-1 constriction elicited in the presence of BQ610 (homopiperidenyl-CO-Leu-D-Trp (CHO)-D-Trp-OH), an endothelin ET(A) receptor antagonist, was enhanced by RES-701-1, and relaxed by BQ788. homopiperidenyl-co-leu-d-trp 117-145 endothelin-1 Oryctolagus cuniculus 56-68 9694914-0 1998 Endothelin-1-induced alterations in phenylephrine-induced contractile responses are largely additive in physiologically diverse rabbit vasculature. Phenylephrine 36-49 endothelin-1 Oryctolagus cuniculus 0-12 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. Calcium 181-188 endothelin-1 Oryctolagus cuniculus 123-135 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. Calcium 181-188 endothelin-1 Oryctolagus cuniculus 137-141 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. isocyanic acid 198-201 endothelin-1 Oryctolagus cuniculus 123-135 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. isocyanic acid 198-201 endothelin-1 Oryctolagus cuniculus 137-141 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. PD 155080 263-271 endothelin-1 Oryctolagus cuniculus 123-135 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. PD 155080 263-271 endothelin-1 Oryctolagus cuniculus 137-141 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. BQ 788 279-285 endothelin-1 Oryctolagus cuniculus 123-135 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. BQ 788 279-285 endothelin-1 Oryctolagus cuniculus 137-141 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. RES-701 287-294 endothelin-1 Oryctolagus cuniculus 123-135 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. RES-701 287-294 endothelin-1 Oryctolagus cuniculus 137-141 9832389-4 1998 A nitric oxide (NO) synthase inhibitor Nomega-nitro-L-arginine methyl ester (L-NAME, 50 microg/kg per min) also potentiated the endothelin-1-induced reductions in urine flow rate and urinary Na+ excretion but not the reduction in renal blood flow. Nitric Oxide 2-14 endothelin-1 Oryctolagus cuniculus 128-140 9832389-4 1998 A nitric oxide (NO) synthase inhibitor Nomega-nitro-L-arginine methyl ester (L-NAME, 50 microg/kg per min) also potentiated the endothelin-1-induced reductions in urine flow rate and urinary Na+ excretion but not the reduction in renal blood flow. NG-Nitroarginine Methyl Ester 39-75 endothelin-1 Oryctolagus cuniculus 128-140 9832389-4 1998 A nitric oxide (NO) synthase inhibitor Nomega-nitro-L-arginine methyl ester (L-NAME, 50 microg/kg per min) also potentiated the endothelin-1-induced reductions in urine flow rate and urinary Na+ excretion but not the reduction in renal blood flow. NG-Nitroarginine Methyl Ester 77-83 endothelin-1 Oryctolagus cuniculus 128-140 9832389-5 1998 Endothelin-1 reduced fractional Na+ excretion in the presence of BQ-788 or L-NAME. BQ 788 65-71 endothelin-1 Oryctolagus cuniculus 0-12 9832389-5 1998 Endothelin-1 reduced fractional Na+ excretion in the presence of BQ-788 or L-NAME. NG-Nitroarginine Methyl Ester 75-81 endothelin-1 Oryctolagus cuniculus 0-12 9700994-5 1998 Cholesterol feeding impaired endothelium-dependent relaxation of rabbit aortic rings ex vivo and increased urinary immunoreactive ET-1 excretion, along with decreased urinary nitrate excretion, an index of NO production. Cholesterol 0-11 endothelin-1 Oryctolagus cuniculus 130-134 9700994-6 1998 L-Arginine partially restored endothelium-dependent relaxation in parallel to increased urinary nitrate excretion and decreased urinary immunoreactive ET-1 excretion. Arginine 0-10 endothelin-1 Oryctolagus cuniculus 151-155 9700994-8 1998 The contractile vascular response of aortic rings to exogenous ET-1 was increased in rabbits fed a high-cholesterol diet; this enhanced contractility to ET-1 was completely reversed by L-arginine. Cholesterol 104-115 endothelin-1 Oryctolagus cuniculus 63-67 9700994-8 1998 The contractile vascular response of aortic rings to exogenous ET-1 was increased in rabbits fed a high-cholesterol diet; this enhanced contractility to ET-1 was completely reversed by L-arginine. Cholesterol 104-115 endothelin-1 Oryctolagus cuniculus 153-157 9700994-8 1998 The contractile vascular response of aortic rings to exogenous ET-1 was increased in rabbits fed a high-cholesterol diet; this enhanced contractility to ET-1 was completely reversed by L-arginine. Arginine 185-195 endothelin-1 Oryctolagus cuniculus 63-67 9700994-8 1998 The contractile vascular response of aortic rings to exogenous ET-1 was increased in rabbits fed a high-cholesterol diet; this enhanced contractility to ET-1 was completely reversed by L-arginine. Arginine 185-195 endothelin-1 Oryctolagus cuniculus 153-157 9700994-9 1998 These data suggest that L-arginine restores endothelial function and normalizes the synthesis and vasoconstrictor response to ET-1 in hypercholesterolemia. Arginine 24-34 endothelin-1 Oryctolagus cuniculus 126-130 9694918-1 1998 The purpose of this study was to establish whether specific receptor subtypes are responsible for mediating the effects of endothelin-1 (ET-1) and endothelin-3 (ET-3) on the L-type calcium current (ICa) using a number of receptor-selective antagonists, including PD155080 (ETA), BQ-788, RES-701 and IRL-1038 (ETB) and the ETA/ETB receptor-non-selective antagonist PD145065. PD 145065 364-372 endothelin-1 Oryctolagus cuniculus 137-141 9694918-5 1998 The decrease in ICa produced by ET-1 was completely blocked by PD155080 and PD145065 (1 and 10 microM); however, ICa was increased upon washout of PD155080. isocyanic acid 16-19 endothelin-1 Oryctolagus cuniculus 32-36 9694918-5 1998 The decrease in ICa produced by ET-1 was completely blocked by PD155080 and PD145065 (1 and 10 microM); however, ICa was increased upon washout of PD155080. PD 145065 76-84 endothelin-1 Oryctolagus cuniculus 32-36 9694918-5 1998 The decrease in ICa produced by ET-1 was completely blocked by PD155080 and PD145065 (1 and 10 microM); however, ICa was increased upon washout of PD155080. isocyanic acid 113-116 endothelin-1 Oryctolagus cuniculus 32-36 9694918-6 1998 Although the decrease in ICa produced by ET-1 was partially blocked by BQ-788 (1 and 10 microM), ET-1 in combination with either RES-701 (1 and 10 microM) or IRL-1038 (1 microM) produced a decrease in ICa similar to that produced by ET-1 alone. isocyanic acid 25-28 endothelin-1 Oryctolagus cuniculus 41-45 9694918-6 1998 Although the decrease in ICa produced by ET-1 was partially blocked by BQ-788 (1 and 10 microM), ET-1 in combination with either RES-701 (1 and 10 microM) or IRL-1038 (1 microM) produced a decrease in ICa similar to that produced by ET-1 alone. BQ 788 71-77 endothelin-1 Oryctolagus cuniculus 41-45 9694918-6 1998 Although the decrease in ICa produced by ET-1 was partially blocked by BQ-788 (1 and 10 microM), ET-1 in combination with either RES-701 (1 and 10 microM) or IRL-1038 (1 microM) produced a decrease in ICa similar to that produced by ET-1 alone. RES-701 129-136 endothelin-1 Oryctolagus cuniculus 41-45 9694918-6 1998 Although the decrease in ICa produced by ET-1 was partially blocked by BQ-788 (1 and 10 microM), ET-1 in combination with either RES-701 (1 and 10 microM) or IRL-1038 (1 microM) produced a decrease in ICa similar to that produced by ET-1 alone. isocyanic acid 201-204 endothelin-1 Oryctolagus cuniculus 41-45 9694918-8 1998 These data indicate that the decrease in ICa produced by ET-1 in rabbit ventricular cardiomyocytes is mediated by the ETA receptor subtype, because PD155080 completely inhibited this response. isocyanic acid 41-44 endothelin-1 Oryctolagus cuniculus 57-61 9694918-8 1998 These data indicate that the decrease in ICa produced by ET-1 in rabbit ventricular cardiomyocytes is mediated by the ETA receptor subtype, because PD155080 completely inhibited this response. PD 155080 148-156 endothelin-1 Oryctolagus cuniculus 57-61 9720787-11 1998 FR139317 inhibited responses to ET-1 in vessels from 0-24 h and 4 day, but not fetal, rabbits (pKb: 6.4 in 4 day rabbits). FR 139317 0-8 endothelin-1 Oryctolagus cuniculus 32-36 9720787-12 1998 BQ-788 inhibited responses to ET-1 at all age points except for 7 days (pKb: 6.7 at 0-24 h; 6.2 at 4 days). BQ 788 0-6 endothelin-1 Oryctolagus cuniculus 30-34 9720787-14 1998 SB 209670 inhibited responses to ET-1 and S6c at 0-24 h and 4 days (pKb for ET-1: 8.3 and 8.0 respectively; pKb for S6c: 9.2 and 10.2 respectively). 1H-Indene-2-carboxylic acid, 1-(1,3-benzodioxol-5-yl)-3-(2- (carboxymethoxy)-4-methoxyphenyl)-2,3-dihydro-5-propoxy-, (1S,2R,3S)- 0-9 endothelin-1 Oryctolagus cuniculus 33-37 9720787-14 1998 SB 209670 inhibited responses to ET-1 and S6c at 0-24 h and 4 days (pKb for ET-1: 8.3 and 8.0 respectively; pKb for S6c: 9.2 and 10.2 respectively). 1H-Indene-2-carboxylic acid, 1-(1,3-benzodioxol-5-yl)-3-(2- (carboxymethoxy)-4-methoxyphenyl)-2,3-dihydro-5-propoxy-, (1S,2R,3S)- 0-9 endothelin-1 Oryctolagus cuniculus 76-80 9593222-6 1998 Moreover, ET-1, ET-3 and SRTX-b completely displaced [3H]BQ-123 with IC50 values of 0.8, 81 and 4.4 nM, respectively, but with slopes of ET-3 and SRTX-b being again shallow. Tritium 54-56 endothelin-1 Oryctolagus cuniculus 10-20 9683027-3 1998 Similarly, 30 nM endothelin-1 induced 30% constriction of vessels pretreated with 1 microM BQ788, and the resulting constriction was completely relaxed by BQ610. BQ 788 91-96 endothelin-1 Oryctolagus cuniculus 17-29 9683027-3 1998 Similarly, 30 nM endothelin-1 induced 30% constriction of vessels pretreated with 1 microM BQ788, and the resulting constriction was completely relaxed by BQ610. BQ 610 155-160 endothelin-1 Oryctolagus cuniculus 17-29 10374426-12 1998 L-NMA and phosphoramidon obviously reduced the plasma levels of NO and ET-1 to below their respective baseline levels, and showed transient effect of increase on blood pressure. l-nma 0-5 endothelin-1 Oryctolagus cuniculus 71-75 10374426-12 1998 L-NMA and phosphoramidon obviously reduced the plasma levels of NO and ET-1 to below their respective baseline levels, and showed transient effect of increase on blood pressure. phosphoramidon 10-24 endothelin-1 Oryctolagus cuniculus 71-75 10374426-14 1998 Dexamethasone just inhibited the excessive increase of NO and ET-1 during endotoxic shock without interfering their baseline levels and showed most beneficial effects on hemodynamics. Dexamethasone 0-13 endothelin-1 Oryctolagus cuniculus 62-66 9593222-6 1998 Moreover, ET-1, ET-3 and SRTX-b completely displaced [3H]BQ-123 with IC50 values of 0.8, 81 and 4.4 nM, respectively, but with slopes of ET-3 and SRTX-b being again shallow. bulaquine 57-59 endothelin-1 Oryctolagus cuniculus 10-20 9184797-19 1997 It seems possible to assume from these results that, although, under the present experimental conditions, nicotine exhibited a tendency to accelerate the intimal hyperplasia after endothelial removal, the longer exposure to nicotine or a higher dose of the agent or both would significantly accelerate the intimal hyperplasia through the enhanced impairment of endothelium-derived relaxing factor/ NO production, which might be brought about by the enhanced increases in L-NMMA and ADMA concentrations, and the enhanced increase in endothelin-1 in the vessel wall. Nicotine 106-114 endothelin-1 Oryctolagus cuniculus 532-544 9608541-3 1998 In aortic EC from normal rabbits, ROS decreased the immunoreactivity of eNOS and ET-1 and this effect was significantly reversed by morin. Reactive Oxygen Species 34-37 endothelin-1 Oryctolagus cuniculus 81-85 9608541-4 1998 In atherosclerotic rabbits, ROS had the same effect on the immunoreactivity of eNOS and ET-1 but also induced the expression of iNOS immunoreactivity. Reactive Oxygen Species 28-31 endothelin-1 Oryctolagus cuniculus 88-92 9595390-1 1998 Endothelin-1 (ET-1) is a 21-amino-acid local and circulating factor whose plasma concentrations are increased in advanced atherosclerosis. 21-amino-acid 25-38 endothelin-1 Oryctolagus cuniculus 0-12 9595390-1 1998 Endothelin-1 (ET-1) is a 21-amino-acid local and circulating factor whose plasma concentrations are increased in advanced atherosclerosis. 21-amino-acid 25-38 endothelin-1 Oryctolagus cuniculus 14-18 9595503-3 1998 Mean ET-1 outflow from ears perfused with fresh Krebs was 4.33 +/- 0.72 pg/min; from ears perfused with coronary effluent it was 84.3% less. krebs 48-53 endothelin-1 Oryctolagus cuniculus 5-9 9595507-2 1998 Using the whole-cell patch-clamp technique in the same preparation, we found that ET-1 reduces other pacemaker currents, the T-type Ca2+ current (ICa,T) and the hyperpolarization-activated inward current (I(f)). isocyanic acid 146-149 endothelin-1 Oryctolagus cuniculus 82-86 9595507-3 1998 The inhibitory actions of ET-1 on these currents were concentration-dependent, i.e., EC50 of 0.9 nM for ICa,T and 2.3 nM for I(f), with little reversal after washout of the peptide. isocyanic acid 104-107 endothelin-1 Oryctolagus cuniculus 26-30 9595418-0 1998 Influence of endothelin-1 on clonidine-induced cardiovascular effects in anesthetized rabbits. Clonidine 29-38 endothelin-1 Oryctolagus cuniculus 13-25 9595418-1 1998 This study was designed to investigate the effect of endothelin-1 (ET-1) on clonidine-induced cardiovascular effects in urethane-anesthetized rabbits and to clarify the mechanism of its action. Clonidine 76-85 endothelin-1 Oryctolagus cuniculus 53-65 9595418-1 1998 This study was designed to investigate the effect of endothelin-1 (ET-1) on clonidine-induced cardiovascular effects in urethane-anesthetized rabbits and to clarify the mechanism of its action. Clonidine 76-85 endothelin-1 Oryctolagus cuniculus 67-71 9595418-1 1998 This study was designed to investigate the effect of endothelin-1 (ET-1) on clonidine-induced cardiovascular effects in urethane-anesthetized rabbits and to clarify the mechanism of its action. Urethane 120-128 endothelin-1 Oryctolagus cuniculus 53-65 9595418-1 1998 This study was designed to investigate the effect of endothelin-1 (ET-1) on clonidine-induced cardiovascular effects in urethane-anesthetized rabbits and to clarify the mechanism of its action. Urethane 120-128 endothelin-1 Oryctolagus cuniculus 67-71 9595418-5 1998 Intravenous clonidine-induced hypotension was significantly enhanced by pretreatment with ET-1 or sarafotoxin, but the bradycardia was not affected. Clonidine 12-21 endothelin-1 Oryctolagus cuniculus 90-94 9595418-11 1998 ET-1 and sarafotoxin was significantly inhibited by nitroprusside but not by phentolamine or sodium acetylsalicylate. Nitroprusside 52-65 endothelin-1 Oryctolagus cuniculus 0-4 9595418-13 1998 Taken together, these experimental data suggest that ET-1 potentiates clonidine-induced hypotensive responses in the urethane-anesthetized rabbit through facilitation of nitric oxide release, which appears to be associated with endothelin receptors. Clonidine 70-79 endothelin-1 Oryctolagus cuniculus 53-57 9595418-13 1998 Taken together, these experimental data suggest that ET-1 potentiates clonidine-induced hypotensive responses in the urethane-anesthetized rabbit through facilitation of nitric oxide release, which appears to be associated with endothelin receptors. Urethane 117-125 endothelin-1 Oryctolagus cuniculus 53-57 9595418-13 1998 Taken together, these experimental data suggest that ET-1 potentiates clonidine-induced hypotensive responses in the urethane-anesthetized rabbit through facilitation of nitric oxide release, which appears to be associated with endothelin receptors. Nitric Oxide 170-182 endothelin-1 Oryctolagus cuniculus 53-57 9537815-2 1997 Prostaglandin E2 caused maximum relaxation of endothelin-1 precontracted vessels (EC50: 1.8 x 10(-8) M). Dinoprostone 0-16 endothelin-1 Oryctolagus cuniculus 46-58 9334651-4 1997 The interaction between NO, PGI2 and endothelin-1 (ET-1), a powerful vasoconstrictor and smooth muscle cell mitogen, is thought to be important in maintaining vascular tone and the erectile process. Epoprostenol 28-32 endothelin-1 Oryctolagus cuniculus 51-55 9335411-4 1997 The contraction by a low concentration of ET-1 was abolished after removal of extracellular Ca2+, but it was reduced only to 50% by a maximally effective concentration (10[-5] M) of nifedipine, an inhibitor of L-type VOCs (L-VOC). Nifedipine 182-192 endothelin-1 Oryctolagus cuniculus 42-46 9335411-5 1997 Mefenamic acid and SK&F 96365 inhibited the ET-1-induced contraction with 50% inhibitory concentration (IC50) values of 10(-4) M and 2 x 10(-5) M, respectively, and abolished it at 10(-3) M and 10(-4) M. By contrast, nifedipine, mefenamic acid, or SK&F 96365 had little effect on the contraction by a high concentration of ET-1. Mefenamic Acid 0-14 endothelin-1 Oryctolagus cuniculus 48-52 9335411-5 1997 Mefenamic acid and SK&F 96365 inhibited the ET-1-induced contraction with 50% inhibitory concentration (IC50) values of 10(-4) M and 2 x 10(-5) M, respectively, and abolished it at 10(-3) M and 10(-4) M. By contrast, nifedipine, mefenamic acid, or SK&F 96365 had little effect on the contraction by a high concentration of ET-1. Mefenamic Acid 0-14 endothelin-1 Oryctolagus cuniculus 331-335 9335411-5 1997 Mefenamic acid and SK&F 96365 inhibited the ET-1-induced contraction with 50% inhibitory concentration (IC50) values of 10(-4) M and 2 x 10(-5) M, respectively, and abolished it at 10(-3) M and 10(-4) M. By contrast, nifedipine, mefenamic acid, or SK&F 96365 had little effect on the contraction by a high concentration of ET-1. amicloral 19-25 endothelin-1 Oryctolagus cuniculus 48-52 9335411-5 1997 Mefenamic acid and SK&F 96365 inhibited the ET-1-induced contraction with 50% inhibitory concentration (IC50) values of 10(-4) M and 2 x 10(-5) M, respectively, and abolished it at 10(-3) M and 10(-4) M. By contrast, nifedipine, mefenamic acid, or SK&F 96365 had little effect on the contraction by a high concentration of ET-1. amicloral 19-25 endothelin-1 Oryctolagus cuniculus 331-335 9335411-5 1997 Mefenamic acid and SK&F 96365 inhibited the ET-1-induced contraction with 50% inhibitory concentration (IC50) values of 10(-4) M and 2 x 10(-5) M, respectively, and abolished it at 10(-3) M and 10(-4) M. By contrast, nifedipine, mefenamic acid, or SK&F 96365 had little effect on the contraction by a high concentration of ET-1. Nifedipine 221-231 endothelin-1 Oryctolagus cuniculus 48-52 9335411-5 1997 Mefenamic acid and SK&F 96365 inhibited the ET-1-induced contraction with 50% inhibitory concentration (IC50) values of 10(-4) M and 2 x 10(-5) M, respectively, and abolished it at 10(-3) M and 10(-4) M. By contrast, nifedipine, mefenamic acid, or SK&F 96365 had little effect on the contraction by a high concentration of ET-1. Mefenamic Acid 233-247 endothelin-1 Oryctolagus cuniculus 48-52 9335411-5 1997 Mefenamic acid and SK&F 96365 inhibited the ET-1-induced contraction with 50% inhibitory concentration (IC50) values of 10(-4) M and 2 x 10(-5) M, respectively, and abolished it at 10(-3) M and 10(-4) M. By contrast, nifedipine, mefenamic acid, or SK&F 96365 had little effect on the contraction by a high concentration of ET-1. amicloral 252-258 endothelin-1 Oryctolagus cuniculus 48-52 9335411-6 1997 The contraction induced by a low or high concentration of ET-1 was abolished by an ETA antagonist, BQ-123, but not by an ETB antagonist, BQ-788. cyclo(Trp-Asp-Pro-Val-Leu) 99-105 endothelin-1 Oryctolagus cuniculus 58-62 9298544-10 1997 At 30 degrees C, phentolamine or yohimbine reduced, prazosin did not modify and alpha, beta-meATP slightly increased the potentiation by endothelin-1 of the response to electrical stimulation. alpha,beta-methyleneadenosine 5'-triphosphate 80-97 endothelin-1 Oryctolagus cuniculus 137-149 9298544-12 1997 The arterial contraction to ATP (2 mM) and the alpha(2)-adrenoceptor agonist BHT-920 (10 microM), but not that to (-)-noradrenaline (1 microM), was potentiated by endothelin-1 at both 37 degrees C and 30 degrees C. 5. Adenosine Triphosphate 28-31 endothelin-1 Oryctolagus cuniculus 163-175 9298544-12 1997 The arterial contraction to ATP (2 mM) and the alpha(2)-adrenoceptor agonist BHT-920 (10 microM), but not that to (-)-noradrenaline (1 microM), was potentiated by endothelin-1 at both 37 degrees C and 30 degrees C. 5. talipexole 77-84 endothelin-1 Oryctolagus cuniculus 163-175 9286621-2 1997 In isolated rabbit right atria, endothelin-1 elicited a negative chronotropic effect in the presence of isoprenaline, which was associated with a decrease in the isoprenaline-induced accumulation of cyclic AMP. Isoproterenol 104-116 endothelin-1 Oryctolagus cuniculus 32-44 9286621-2 1997 In isolated rabbit right atria, endothelin-1 elicited a negative chronotropic effect in the presence of isoprenaline, which was associated with a decrease in the isoprenaline-induced accumulation of cyclic AMP. Isoproterenol 162-174 endothelin-1 Oryctolagus cuniculus 32-44 9286621-2 1997 In isolated rabbit right atria, endothelin-1 elicited a negative chronotropic effect in the presence of isoprenaline, which was associated with a decrease in the isoprenaline-induced accumulation of cyclic AMP. Cyclic AMP 199-209 endothelin-1 Oryctolagus cuniculus 32-44 9283700-7 1997 This potentiation by endothelin-1 was reduced by the antagonist for endothelin ETA receptors BQ-123 (10 microM) but not by the antagonist for endothelin ETB receptors BQ-788 (10 microM). cyclo(Trp-Asp-Pro-Val-Leu) 93-99 endothelin-1 Oryctolagus cuniculus 21-33 9283700-12 1997 Endothelium removal increased the contraction to electrical stimulation at 30 degrees C (about 91% for 8 Hz) but not at 37 degrees C. Both L-NOARG and endothelium removal abolished the potentiating effects of endothelin-1 on the response to electrical stimulation found at 30 degrees C. 6. Nitroarginine 139-146 endothelin-1 Oryctolagus cuniculus 209-221 9283700-14 1997 This potentiating effect of endothelin-1 may require the presence of an inhibitory tone due to endothelial nitric oxide. Nitric Oxide 107-119 endothelin-1 Oryctolagus cuniculus 28-40 9249482-1 1997 In isolated rabbit right atria, endothelin (ET) isopeptides ET-1 and ET-3 elicited a concentration-dependent negative chronotropic effect (NCE) in the presence of isoproterenol (Iso): ET-1 was approximately 10 times more potent than ET-3. Isoproterenol 163-176 endothelin-1 Oryctolagus cuniculus 60-64 9249482-1 1997 In isolated rabbit right atria, endothelin (ET) isopeptides ET-1 and ET-3 elicited a concentration-dependent negative chronotropic effect (NCE) in the presence of isoproterenol (Iso): ET-1 was approximately 10 times more potent than ET-3. Isoproterenol 163-176 endothelin-1 Oryctolagus cuniculus 184-188 9249482-3 1997 ET-1 decreased the adenosine 3",5"-cyclic monophosphate (cAMP) level in the presence of Iso in rabbit atria. Cyclic AMP 19-55 endothelin-1 Oryctolagus cuniculus 0-4 9249482-3 1997 ET-1 decreased the adenosine 3",5"-cyclic monophosphate (cAMP) level in the presence of Iso in rabbit atria. Cyclic AMP 57-61 endothelin-1 Oryctolagus cuniculus 0-4 9751446-3 1998 Conformers generated from the D2O-H2O data superposed on the corresponding main-chain atoms in the crystal structure of endothelin 1 and the solution structure of BQ-123 with root mean square co-ordinate differences of 0.9A and 0.77A, respectively. Deuterium Oxide 30-33 endothelin-1 Oryctolagus cuniculus 120-132 9751446-3 1998 Conformers generated from the D2O-H2O data superposed on the corresponding main-chain atoms in the crystal structure of endothelin 1 and the solution structure of BQ-123 with root mean square co-ordinate differences of 0.9A and 0.77A, respectively. Water 34-37 endothelin-1 Oryctolagus cuniculus 120-132 9751446-5 1998 Because the peptide can adopt a conformer which closely matches the equivalent residues in the endothelin 1 crystal structure and in BQ-123, we suggest BQ-123 does not necessarily mimic the endothelin C-terminal region to achieve its antagonism, and that a helical conformation of the endothelin C-terminal hexapeptide does not favour its interaction at the endothelin B receptor. cyclo(Trp-Asp-Pro-Val-Leu) 152-158 endothelin-1 Oryctolagus cuniculus 95-107 9523100-0 1998 Inflammatory reaction via arachidonic acid cascade after intravitreal injection of endothelin-1. Arachidonic Acid 26-42 endothelin-1 Oryctolagus cuniculus 83-95 9595468-0 1998 Tetramethylpyrazine, a Chinese drug, blocks coronary vasoconstriction by endothelin-1 and decreases plasma endothelin-1 levels in experimental animals. tetramethylpyrazine 0-19 endothelin-1 Oryctolagus cuniculus 73-85 9595468-0 1998 Tetramethylpyrazine, a Chinese drug, blocks coronary vasoconstriction by endothelin-1 and decreases plasma endothelin-1 levels in experimental animals. tetramethylpyrazine 0-19 endothelin-1 Oryctolagus cuniculus 107-119 9595468-1 1998 The purpose of this study was to investigate effects of tetramethylpyrazine (TMP), a Chinese plant-derived medicine, on coronary vasoconstriction and related electrocardiographic and histologic changes caused by endothelin-1 (ET-1), and on plasma ET-1 levels. tetramethylpyrazine 56-75 endothelin-1 Oryctolagus cuniculus 212-224 9595468-1 1998 The purpose of this study was to investigate effects of tetramethylpyrazine (TMP), a Chinese plant-derived medicine, on coronary vasoconstriction and related electrocardiographic and histologic changes caused by endothelin-1 (ET-1), and on plasma ET-1 levels. tetramethylpyrazine 56-75 endothelin-1 Oryctolagus cuniculus 226-230 9595468-1 1998 The purpose of this study was to investigate effects of tetramethylpyrazine (TMP), a Chinese plant-derived medicine, on coronary vasoconstriction and related electrocardiographic and histologic changes caused by endothelin-1 (ET-1), and on plasma ET-1 levels. tetramethylpyrazine 56-75 endothelin-1 Oryctolagus cuniculus 247-251 9595468-1 1998 The purpose of this study was to investigate effects of tetramethylpyrazine (TMP), a Chinese plant-derived medicine, on coronary vasoconstriction and related electrocardiographic and histologic changes caused by endothelin-1 (ET-1), and on plasma ET-1 levels. tetramethylpyrazine 77-80 endothelin-1 Oryctolagus cuniculus 212-224 9595468-1 1998 The purpose of this study was to investigate effects of tetramethylpyrazine (TMP), a Chinese plant-derived medicine, on coronary vasoconstriction and related electrocardiographic and histologic changes caused by endothelin-1 (ET-1), and on plasma ET-1 levels. tetramethylpyrazine 77-80 endothelin-1 Oryctolagus cuniculus 226-230 9595468-1 1998 The purpose of this study was to investigate effects of tetramethylpyrazine (TMP), a Chinese plant-derived medicine, on coronary vasoconstriction and related electrocardiographic and histologic changes caused by endothelin-1 (ET-1), and on plasma ET-1 levels. tetramethylpyrazine 77-80 endothelin-1 Oryctolagus cuniculus 247-251 9595468-8 1998 Intracoronary injection of ET-1 resulted in a significant vasoconstriction of the entire vascular bed of the LCA, with a decrease in CAD of 35.9 +/- 5.7% (n = 5; p < 0.01) and ischemic changes on ECG and in tissues of endocardium, myocardium, coronary endothelial cells, and capillary vessels. Lithocholic Acid 109-112 endothelin-1 Oryctolagus cuniculus 27-31 9595468-9 1998 Pretreatment with TMP produced a significant increase in CAD by 38.5 +/- 7.8% (n = 5; p < 0.01) and greatly suppressed the vasoconstriction produced by ET-1. tetramethylpyrazine 18-21 endothelin-1 Oryctolagus cuniculus 155-159 9595468-10 1998 The myocardial tissue damage estimated from the ratio of ischemic area for the entire area after ET-1 injection (35.6%) was completely abolished by TMP (0.6%). tetramethylpyrazine 148-151 endothelin-1 Oryctolagus cuniculus 97-101 9595468-11 1998 In addition, TMP injection induced a significant decrease in plasma ET-1 levels and an increase in 6-keto-PGF1 levels in rabbits. tetramethylpyrazine 13-16 endothelin-1 Oryctolagus cuniculus 68-72 9595468-12 1998 The Chinese medicine TMP could be a useful therapeutic agent in ischemic heart disease by suppressing coronary vasoconstriction and ischemic changes in the tissues produced by ET-1. tetramethylpyrazine 21-24 endothelin-1 Oryctolagus cuniculus 176-180 9595427-0 1998 Endothelin-B receptor-dependent modulation of the pressor and prostacyclin-releasing properties of dynamically converted big endothelin-1 in the anesthetized rabbit. Epoprostenol 62-74 endothelin-1 Oryctolagus cuniculus 125-137 9595427-2 1998 In this study we investigated the effect of selective ETA or ETB receptor blockade on the pressor response and increase in plasma prostacyclin (PGI2) levels (determined by RIA) induced by big ET-1 in the anesthetized rabbit. Epoprostenol 130-142 endothelin-1 Oryctolagus cuniculus 192-196 9595427-2 1998 In this study we investigated the effect of selective ETA or ETB receptor blockade on the pressor response and increase in plasma prostacyclin (PGI2) levels (determined by RIA) induced by big ET-1 in the anesthetized rabbit. Epoprostenol 144-148 endothelin-1 Oryctolagus cuniculus 192-196 9595427-3 1998 Pretreatment (5 min) of the rabbit with the ETB receptor antagonist BQ-788 (0.25 mg/kg) potentiated the ET-1 (1 nmol/kg) and, interestingly, big ET-1 (0.5 nmol/kg) induced pressor responses. BQ 788 68-74 endothelin-1 Oryctolagus cuniculus 104-108 9595427-3 1998 Pretreatment (5 min) of the rabbit with the ETB receptor antagonist BQ-788 (0.25 mg/kg) potentiated the ET-1 (1 nmol/kg) and, interestingly, big ET-1 (0.5 nmol/kg) induced pressor responses. BQ 788 68-74 endothelin-1 Oryctolagus cuniculus 145-149 9595427-4 1998 The selective ETA receptor antagonist BQ-123 (1 mg/kg) significantly reduced the big ET-1 (0.5 and 3 nmol/kg) pressor responses. cyclo(Trp-Asp-Pro-Val-Leu) 38-44 endothelin-1 Oryctolagus cuniculus 85-89 9595427-10 1998 administration of big ET-1 (3 nmol/kg) induced a significant release of PGI2. Epoprostenol 72-76 endothelin-1 Oryctolagus cuniculus 22-26 9595427-12 1998 Furthermore, after pulmonary conversion of big ET-1, ETA receptors may be responsible for the release of vasodilator and anti-aggregatory prostacyclin, which modulates the big ET-1-induced responses in the rabbit. Epoprostenol 138-150 endothelin-1 Oryctolagus cuniculus 47-51 9595427-12 1998 Furthermore, after pulmonary conversion of big ET-1, ETA receptors may be responsible for the release of vasodilator and anti-aggregatory prostacyclin, which modulates the big ET-1-induced responses in the rabbit. Epoprostenol 138-150 endothelin-1 Oryctolagus cuniculus 176-180 9595429-3 1998 In such ETA/ETB receptor composite-type tissues, low doses of the ETB antagonist BQ-788 shifted the concentration-contraction curve for ET-1 slightly to the left, implying potentiation of contraction. BQ 788 81-87 endothelin-1 Oryctolagus cuniculus 136-140 9595429-7 1998 In contrast, the ETB-selective antagonist BQ-788 (10(-8) M to 10(-7) M) enhanced contraction in ET-1-precontracted artery. BQ 788 42-48 endothelin-1 Oryctolagus cuniculus 96-100 9374700-7 1997 The ET-1-induced hepatic vasoconstriction was significantly attenuated by the selective ETA receptor antagonist BQ-123 (1 microM). cyclo(Trp-Asp-Pro-Val-Leu) 112-118 endothelin-1 Oryctolagus cuniculus 4-8 9374700-8 1997 The ETB receptor antagonist BQ-788 (1 microM) also attenuated the constriction at ET-1 concentrations less than 10 nM. BQ 788 28-34 endothelin-1 Oryctolagus cuniculus 82-86 9369277-1 1997 Endothelin-1 (0.25 nmol/kg, injected into the left cardiac ventricle) induces a protracted increase of mean arterial pressure that is significantly reduced by the selective ET(A) receptor antagonist BQ-123 (1 and 10 mg/kg) in the anesthetized rabbit. cyclo(Trp-Asp-Pro-Val-Leu) 199-205 endothelin-1 Oryctolagus cuniculus 0-12 9369277-3 1997 Concomitant to the increase in mean arterial pressure, BQ-788 induces a significant increase in plasma levels of endothelin-1 and its precursor big endothelin-1. BQ 788 55-61 endothelin-1 Oryctolagus cuniculus 113-125 9369277-3 1997 Concomitant to the increase in mean arterial pressure, BQ-788 induces a significant increase in plasma levels of endothelin-1 and its precursor big endothelin-1. BQ 788 55-61 endothelin-1 Oryctolagus cuniculus 148-160 9369277-7 1997 Finally, L-NAME induces an increase in plasma levels of big endothelin-1 but not endothelin-1. NG-Nitroarginine Methyl Ester 9-15 endothelin-1 Oryctolagus cuniculus 60-72 9321831-2 1997 Endothelin-1 (ET-1) may interact with nitric oxide pathways. Nitric Oxide 38-50 endothelin-1 Oryctolagus cuniculus 0-12 9321831-2 1997 Endothelin-1 (ET-1) may interact with nitric oxide pathways. Nitric Oxide 38-50 endothelin-1 Oryctolagus cuniculus 14-18 9321831-6 1997 Coincubation with ET-1 (10 nM) attenuated the contractile depression and increase in cGMP with LPS (482 +/- 28 fmol/mg protein, P < 0.05 vs. LPS alone). Cyclic GMP 85-89 endothelin-1 Oryctolagus cuniculus 18-22 9321831-8 1997 ET-1 effects on contractile function were blocked by BQ-123 (10 microM), a selective ET-1 type A receptor antagonist. cyclo(Trp-Asp-Pro-Val-Leu) 53-59 endothelin-1 Oryctolagus cuniculus 0-4 9321831-8 1997 ET-1 effects on contractile function were blocked by BQ-123 (10 microM), a selective ET-1 type A receptor antagonist. cyclo(Trp-Asp-Pro-Val-Leu) 53-59 endothelin-1 Oryctolagus cuniculus 85-89 9321831-9 1997 We conclude that ET-1 ameliorates LPS-induced contractile depression in cardiac myocytes by attenuating LPS effects on nitric oxide-cGMP pathways. Nitric Oxide 119-131 endothelin-1 Oryctolagus cuniculus 17-21 9321831-9 1997 We conclude that ET-1 ameliorates LPS-induced contractile depression in cardiac myocytes by attenuating LPS effects on nitric oxide-cGMP pathways. Cyclic GMP 132-136 endothelin-1 Oryctolagus cuniculus 17-21 9298544-9 1997 At 37 degrees C, endothelin-1 in the presence of phentolamine or prazosin, but not in that of yohimbine, alpha, beta-meATP or PPADS, potentiated the contraction to electrical stimulation. Phentolamine 49-61 endothelin-1 Oryctolagus cuniculus 17-29 9298544-9 1997 At 37 degrees C, endothelin-1 in the presence of phentolamine or prazosin, but not in that of yohimbine, alpha, beta-meATP or PPADS, potentiated the contraction to electrical stimulation. Prazosin 65-73 endothelin-1 Oryctolagus cuniculus 17-29 9313942-10 1997 These ET-1-induced changes in membrane currents were abolished by BQ485 (0.3 microM), a highly selective ETA receptor antagonist. BQ 485 66-71 endothelin-1 Oryctolagus cuniculus 6-10 9313942-12 1997 When Ica(L) was inhibited by ET-1 (1 nM), subsequent application of 10 microM ACh showed no additional decrease in Ica(L), suggesting the involvement of cyclic AMP in the effects of ET-1 on Ica(L). isocyanic acid 5-8 endothelin-1 Oryctolagus cuniculus 29-33 9313942-12 1997 When Ica(L) was inhibited by ET-1 (1 nM), subsequent application of 10 microM ACh showed no additional decrease in Ica(L), suggesting the involvement of cyclic AMP in the effects of ET-1 on Ica(L). Cyclic AMP 153-163 endothelin-1 Oryctolagus cuniculus 182-186 9313942-13 1997 In contrast, 1 nM ET-1 further decreased Ica(L) in the presence of 10 microM ACh, suggesting that ET-1 activates some additional mechanism(s) which inhibit Ica(L). Acetylcholine 77-80 endothelin-1 Oryctolagus cuniculus 18-22 9313942-13 1997 In contrast, 1 nM ET-1 further decreased Ica(L) in the presence of 10 microM ACh, suggesting that ET-1 activates some additional mechanism(s) which inhibit Ica(L). Acetylcholine 77-80 endothelin-1 Oryctolagus cuniculus 98-102 9313942-14 1997 The ET-1-induced Ica(L) inhibition was abolished by protein kinase A inhibitory peptide (PKI, 20 microM) or H-89 (5 microM). isocyanic acid 17-20 endothelin-1 Oryctolagus cuniculus 4-8 9313942-14 1997 The ET-1-induced Ica(L) inhibition was abolished by protein kinase A inhibitory peptide (PKI, 20 microM) or H-89 (5 microM). N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide 108-112 endothelin-1 Oryctolagus cuniculus 4-8 9313942-25 1997 The inhibition of ICa(L) by ET-1 is mainly due to reduction of the cyclicAMP levels via PTX-sensitive G protein, but some other mechanism(s) also seems to be operative. Cyclic AMP 67-76 endothelin-1 Oryctolagus cuniculus 28-32 9313942-25 1997 The inhibition of ICa(L) by ET-1 is mainly due to reduction of the cyclicAMP levels via PTX-sensitive G protein, but some other mechanism(s) also seems to be operative. ptx 88-91 endothelin-1 Oryctolagus cuniculus 28-32 9184797-19 1997 It seems possible to assume from these results that, although, under the present experimental conditions, nicotine exhibited a tendency to accelerate the intimal hyperplasia after endothelial removal, the longer exposure to nicotine or a higher dose of the agent or both would significantly accelerate the intimal hyperplasia through the enhanced impairment of endothelium-derived relaxing factor/ NO production, which might be brought about by the enhanced increases in L-NMMA and ADMA concentrations, and the enhanced increase in endothelin-1 in the vessel wall. Nicotine 224-232 endothelin-1 Oryctolagus cuniculus 532-544 9150852-12 1997 In rabbit basilar arteries, AJ-3941 caused a dose-dependent inhibition of the contraction induced by various vasospasmogens, such as endothelin-1 (ET), arachidonic acid, 15-hydroperoxy-eicosatetraenoic acid and the thromboxane A2-mimetic U-46619. AJ 3941 28-35 endothelin-1 Oryctolagus cuniculus 133-145 9125673-2 1997 ET-1 (> or =100 pM) increased significantly total mass of cellular protein and incorporation of L-U-[(14)C]phenylalanine and 2-[(14)C]uridine into cellular protein and RNA, respectively. l-u-[(14)c]phenylalanine 99-123 endothelin-1 Oryctolagus cuniculus 0-4 9136569-4 1997 Increases in aqueous PGE2 concentration were observed after the injection of ET-1, which was inhibited by pre-treatment with 97-139. Dinoprostone 21-25 endothelin-1 Oryctolagus cuniculus 77-81 9136569-6 1997 These results indicate that the effects of ET-1 on APC are at least partially mediated by the cyclooxygenase pathway of arachidonic acid cascade, and that ETA receptors play an important role in these reactions. Arachidonic Acid 120-136 endothelin-1 Oryctolagus cuniculus 43-47 9085040-6 1997 At 0.01 and 0.003 mg/kg per h, PD 156707 also inhibited endothelin-1 induced increases in renal vascular resistance but the effects were less striking, leading to the conclusion that the minimum effective intravenous dose of the compound in rabbits is in the range of 0.01-0.03 mg/kg per h. The results of this study demonstrate that PD 156707 is an extremely potent and highly selective endothelin ETA receptor antagonist. PD 156707 31-40 endothelin-1 Oryctolagus cuniculus 56-68 9125673-2 1997 ET-1 (> or =100 pM) increased significantly total mass of cellular protein and incorporation of L-U-[(14)C]phenylalanine and 2-[(14)C]uridine into cellular protein and RNA, respectively. 2-[(14)c]uridine 128-144 endothelin-1 Oryctolagus cuniculus 0-4 9125673-3 1997 Cycloheximide (35 microM), an inhibitor of protein synthesis, significantly reduced the incorporation of L-U-[(14)C]phenylalanine and 2-[(14)C]uridine into cellular protein and RNA, respectively, under control conditions and in response to ET-1. Cycloheximide 0-13 endothelin-1 Oryctolagus cuniculus 240-244 9125673-3 1997 Cycloheximide (35 microM), an inhibitor of protein synthesis, significantly reduced the incorporation of L-U-[(14)C]phenylalanine and 2-[(14)C]uridine into cellular protein and RNA, respectively, under control conditions and in response to ET-1. l-u-[(14)c]phenylalanine 105-129 endothelin-1 Oryctolagus cuniculus 240-244 9125673-4 1997 Actinomycin D (5 microM), a selective inhibitor of transcription, abolished the incorporation of 2-[(14)C]uridine into cellular RNA and significantly reduced the incorporation of L-U-[(14)C]phenylalanine into cellular protein under control conditions and in response to ET-1. Dactinomycin 0-13 endothelin-1 Oryctolagus cuniculus 270-274 9125673-4 1997 Actinomycin D (5 microM), a selective inhibitor of transcription, abolished the incorporation of 2-[(14)C]uridine into cellular RNA and significantly reduced the incorporation of L-U-[(14)C]phenylalanine into cellular protein under control conditions and in response to ET-1. l-u-[(14)c]phenylalanine 179-203 endothelin-1 Oryctolagus cuniculus 270-274 9125673-5 1997 The selective antagonists at the ET(A) receptor [BQ123 (100 nM) and PD155080 (100 nM)] and the selective antagonist at the ET(B) receptor [BQ788 (100 nM)] significantly reduced the incorporation of L-U-[(14)C]phenylalanine into cellular protein in response to ET-1 (10 nM). BQ 788 139-144 endothelin-1 Oryctolagus cuniculus 260-264 9125673-5 1997 The selective antagonists at the ET(A) receptor [BQ123 (100 nM) and PD155080 (100 nM)] and the selective antagonist at the ET(B) receptor [BQ788 (100 nM)] significantly reduced the incorporation of L-U-[(14)C]phenylalanine into cellular protein in response to ET-1 (10 nM). l-u-[(14)c]phenylalanine 198-222 endothelin-1 Oryctolagus cuniculus 260-264 9125673-6 1997 The selective inhibitor of protein kinase C (PKC), bisindolylmaleimide (BIM) (5 microM), reduced markedly the incorporation of 2-[(14)C]uridine into cellular RNA and, to a lesser degree, the incorporation of L-U-[(14)C]phenylalanine into cellular protein in response to ET-1 (100 pM to 10 nM). bisindolylmaleimide 51-70 endothelin-1 Oryctolagus cuniculus 270-274 9125673-6 1997 The selective inhibitor of protein kinase C (PKC), bisindolylmaleimide (BIM) (5 microM), reduced markedly the incorporation of 2-[(14)C]uridine into cellular RNA and, to a lesser degree, the incorporation of L-U-[(14)C]phenylalanine into cellular protein in response to ET-1 (100 pM to 10 nM). bisindolylmaleimide 72-75 endothelin-1 Oryctolagus cuniculus 270-274 9009214-2 1997 We report that endothelin-1 stimulation of rabbit platelets resulted in a dose- and time-dependent tyrosine phosphorylation of four groups of proteins in the molecular mass ranges of 50, 60, 70-100 and 100-200 kDa and that one of these corresponds to focal adhesion kinase. Tyrosine 99-107 endothelin-1 Oryctolagus cuniculus 15-27 9086738-5 1997 Treatment with anti-prostaglandin agents before and after the injection blocked APC increase completely in the 10(-5)M ET-1 model, and partially in the 10(-4)M ET-1 model. Prostaglandins 20-33 endothelin-1 Oryctolagus cuniculus 119-123 9086738-5 1997 Treatment with anti-prostaglandin agents before and after the injection blocked APC increase completely in the 10(-5)M ET-1 model, and partially in the 10(-4)M ET-1 model. Prostaglandins 20-33 endothelin-1 Oryctolagus cuniculus 160-164 9086738-6 1997 These results indicate that ET-1 effects on APC are at least partially mediated by the cyclooxygenase pathway of the arachidonic acid cascade. Arachidonic Acid 117-133 endothelin-1 Oryctolagus cuniculus 28-32 9009214-7 1997 As a consequence, modulation and regulation by endothelin-1 in rabbit platelets can be proposed through a cAMP-dependent pathway and a tyrosine phosphorylation process that may affect some relevant proteins such as focal adhesion kinase. Cyclic AMP 106-110 endothelin-1 Oryctolagus cuniculus 47-59 9009214-7 1997 As a consequence, modulation and regulation by endothelin-1 in rabbit platelets can be proposed through a cAMP-dependent pathway and a tyrosine phosphorylation process that may affect some relevant proteins such as focal adhesion kinase. Tyrosine 135-143 endothelin-1 Oryctolagus cuniculus 47-59 9052846-6 1997 The fractional excretion of ET-1 increased from 10 to 89% at 48 h in the uranyl acetate treated group and from 6 to 15% at 24 h in the renal artery clamping group, suggesting the existence of ET-1 secretion from the nephron in both types of ARF. uranyl acetate 73-87 endothelin-1 Oryctolagus cuniculus 28-32 9117126-15 1997 The response to the third infusion of endothelin-1 following cessation of infusion of BQ-123, BMS 182874 and SB 209670 was not significantly different from that to the third infusion of endothelin in control conditions. cyclo(Trp-Asp-Pro-Val-Leu) 86-92 endothelin-1 Oryctolagus cuniculus 38-50 9117126-16 1997 However, the response to endothelin-1 was significantly higher than control in tissues pre-infused with BQ-788 or BQ-928 (56 +/- 9 and 41.6 +/- 15%, respectively, n = 8 each, P < 0.05). BQ 788 104-110 endothelin-1 Oryctolagus cuniculus 25-37 9117126-16 1997 However, the response to endothelin-1 was significantly higher than control in tissues pre-infused with BQ-788 or BQ-928 (56 +/- 9 and 41.6 +/- 15%, respectively, n = 8 each, P < 0.05). BQ 928 114-120 endothelin-1 Oryctolagus cuniculus 25-37 9117126-20 1997 In contrast, ETB selective antagonists or mixed antagonists possessing a high affinity for ETB receptors (such as BQ-928) interfere with the ETB-receptor-dependent physiological antagonism of endothelin-1-induced pressor responses in these same tissues. BQ 928 114-120 endothelin-1 Oryctolagus cuniculus 192-204 9052846-6 1997 The fractional excretion of ET-1 increased from 10 to 89% at 48 h in the uranyl acetate treated group and from 6 to 15% at 24 h in the renal artery clamping group, suggesting the existence of ET-1 secretion from the nephron in both types of ARF. uranyl acetate 73-87 endothelin-1 Oryctolagus cuniculus 192-196 9147187-0 1997 Nicardipine modification of endothelin-1 effects on visual evoked potential. Nicardipine 0-11 endothelin-1 Oryctolagus cuniculus 28-40 9147187-3 1997 We examined the antagonistic effect of a calcium-ion channel blocker, nicardipine, on ET-1 effects on visual evoked potential (VEP). Nicardipine 70-81 endothelin-1 Oryctolagus cuniculus 86-90 9147187-8 1997 Nicardipine suppressed the ET-1-induced reduction of VEP amplitude (P < 0.05, Scheffe). Nicardipine 0-11 endothelin-1 Oryctolagus cuniculus 27-31 9147187-10 1997 Since nicardipine interferes with the activity of ET-1, we believe that Ca2+ channel blockers can be useful in the treatment of ischemic retinal and optic nerve disorders that are related to abnormal ET-1 production. Nicardipine 6-17 endothelin-1 Oryctolagus cuniculus 50-54 9147187-10 1997 Since nicardipine interferes with the activity of ET-1, we believe that Ca2+ channel blockers can be useful in the treatment of ischemic retinal and optic nerve disorders that are related to abnormal ET-1 production. Nicardipine 6-17 endothelin-1 Oryctolagus cuniculus 200-204 8893732-3 1996 The last of these steps involves the proteolytic conversion of a relatively inactive propeptide, Big ET-1, to its active, 21-amino acid peptide form. propeptide 85-95 endothelin-1 Oryctolagus cuniculus 101-105 9148280-3 1997 The nitric oxide synthase inhibitor N omega-nitro-L-arginine (0.1 mM) also increased the sensitivity to endothelin-1 (0.6 log units) in basilar arteries with endothelium, whereas N omega-nitro-D-arginine (0.1 mM) and indomethacin (3 microM) had no effect, indicating that withdrawal of endothelium-derived nitric oxide may account for the enhancement of the endothelin-1-induced contraction after endothelial denudation. Nitric Oxide 4-16 endothelin-1 Oryctolagus cuniculus 104-116 9148280-3 1997 The nitric oxide synthase inhibitor N omega-nitro-L-arginine (0.1 mM) also increased the sensitivity to endothelin-1 (0.6 log units) in basilar arteries with endothelium, whereas N omega-nitro-D-arginine (0.1 mM) and indomethacin (3 microM) had no effect, indicating that withdrawal of endothelium-derived nitric oxide may account for the enhancement of the endothelin-1-induced contraction after endothelial denudation. Nitric Oxide 4-16 endothelin-1 Oryctolagus cuniculus 358-370 9148280-3 1997 The nitric oxide synthase inhibitor N omega-nitro-L-arginine (0.1 mM) also increased the sensitivity to endothelin-1 (0.6 log units) in basilar arteries with endothelium, whereas N omega-nitro-D-arginine (0.1 mM) and indomethacin (3 microM) had no effect, indicating that withdrawal of endothelium-derived nitric oxide may account for the enhancement of the endothelin-1-induced contraction after endothelial denudation. Nitroarginine 36-60 endothelin-1 Oryctolagus cuniculus 104-116 9148280-3 1997 The nitric oxide synthase inhibitor N omega-nitro-L-arginine (0.1 mM) also increased the sensitivity to endothelin-1 (0.6 log units) in basilar arteries with endothelium, whereas N omega-nitro-D-arginine (0.1 mM) and indomethacin (3 microM) had no effect, indicating that withdrawal of endothelium-derived nitric oxide may account for the enhancement of the endothelin-1-induced contraction after endothelial denudation. Nitroarginine 36-60 endothelin-1 Oryctolagus cuniculus 358-370 9148280-3 1997 The nitric oxide synthase inhibitor N omega-nitro-L-arginine (0.1 mM) also increased the sensitivity to endothelin-1 (0.6 log units) in basilar arteries with endothelium, whereas N omega-nitro-D-arginine (0.1 mM) and indomethacin (3 microM) had no effect, indicating that withdrawal of endothelium-derived nitric oxide may account for the enhancement of the endothelin-1-induced contraction after endothelial denudation. Indomethacin 217-229 endothelin-1 Oryctolagus cuniculus 104-116 9148280-3 1997 The nitric oxide synthase inhibitor N omega-nitro-L-arginine (0.1 mM) also increased the sensitivity to endothelin-1 (0.6 log units) in basilar arteries with endothelium, whereas N omega-nitro-D-arginine (0.1 mM) and indomethacin (3 microM) had no effect, indicating that withdrawal of endothelium-derived nitric oxide may account for the enhancement of the endothelin-1-induced contraction after endothelial denudation. Nitric Oxide 306-318 endothelin-1 Oryctolagus cuniculus 104-116 9148280-4 1997 Pinacidil (1 microM) shifted the concentration-response curve for endothelin-1 to the right without affecting the maximal response in arteries without endothelium, but had no effect on the endothelin-1-induced contraction in vessels with endothelium. Pinacidil 0-9 endothelin-1 Oryctolagus cuniculus 66-78 9148280-5 1997 Nimodipine (1 microM) reduced the maximal endothelin-1-induced contraction by approximately 50% in both the presence and absence of endothelium, whereas the sensitivity to endothelin-1 was reduced only in vessels without endothelium. Nimodipine 0-10 endothelin-1 Oryctolagus cuniculus 42-54 9148280-6 1997 Incubation in "calcium-free" medium reduced the maximal endothelin-1-induced contraction by 69% and 80% in vessels with and without endothelium, respectively. Calcium 15-22 endothelin-1 Oryctolagus cuniculus 56-68 8968552-4 1996 In this study, the effects of endothelin-1 (ET-1) on the acetylcholine-activated potassium current (IK(ACh) were investigated in the absence and presence of the receptor-selective antagonists, PD155080 (ETA receptor-selective) and RES-701 (ETB receptor-selective) in rabbit atrial cardiomyocytes. Acetylcholine 57-70 endothelin-1 Oryctolagus cuniculus 30-42 8968552-4 1996 In this study, the effects of endothelin-1 (ET-1) on the acetylcholine-activated potassium current (IK(ACh) were investigated in the absence and presence of the receptor-selective antagonists, PD155080 (ETA receptor-selective) and RES-701 (ETB receptor-selective) in rabbit atrial cardiomyocytes. Acetylcholine 57-70 endothelin-1 Oryctolagus cuniculus 44-48 8968552-12 1996 Acetylcholine also increased the holding current at -40 mV from +80 +/- 9 pA to +242 +/- 38 pA, and this effect was abolished (P < 0.05) in the presence of endothelin-1 (+44 +/- 13 pA). Acetylcholine 0-13 endothelin-1 Oryctolagus cuniculus 159-171 8968552-14 1996 Endothelin-1 (10 nM) in the presence of acetylcholine did not affect the "steady state" potassium current (-882 +/- 88 pA compared to a control value of -870 +/- 98 pA, at -100 mV). Acetylcholine 40-53 endothelin-1 Oryctolagus cuniculus 0-12 8968552-16 1996 The ETA receptor-selective antagonist, PD155080 (1 microM), prevented (P < 0.05) the ET-1 induced inhibition of IK(ACh) at all potentials. PD 155080 39-47 endothelin-1 Oryctolagus cuniculus 88-92 8968552-16 1996 The ETA receptor-selective antagonist, PD155080 (1 microM), prevented (P < 0.05) the ET-1 induced inhibition of IK(ACh) at all potentials. Acetylcholine 118-121 endothelin-1 Oryctolagus cuniculus 88-92 8968552-17 1996 PD155080, in the presence of endothelin-1 and acetylcholine, increased the inward component of the "steady state" potassium current to -1030 +/- 210 pA from a control value of -804 +/- 224 pA at a step potential of -100 mV. PD 155080 0-8 endothelin-1 Oryctolagus cuniculus 29-41 8968552-17 1996 PD155080, in the presence of endothelin-1 and acetylcholine, increased the inward component of the "steady state" potassium current to -1030 +/- 210 pA from a control value of -804 +/- 224 pA at a step potential of -100 mV. Potassium 114-123 endothelin-1 Oryctolagus cuniculus 29-41 8968552-19 1996 Unlike PD155080, the ETB receptor-selective antagonist, RES-701 (1 microM), only prevented (P < 0.05) the inhibitory effect of endothelin-1 on the inward component of the IK(ACh); at -100 mV, RES-701, in the presence of endothelin-1 and acetylcholine, increased the "steady state" potassium current to -913 +/- 137 pA from -733 +/- 116 pA. RES-701 56-63 endothelin-1 Oryctolagus cuniculus 130-142 8968552-19 1996 Unlike PD155080, the ETB receptor-selective antagonist, RES-701 (1 microM), only prevented (P < 0.05) the inhibitory effect of endothelin-1 on the inward component of the IK(ACh); at -100 mV, RES-701, in the presence of endothelin-1 and acetylcholine, increased the "steady state" potassium current to -913 +/- 137 pA from -733 +/- 116 pA. RES-701 56-63 endothelin-1 Oryctolagus cuniculus 223-235 8968552-19 1996 Unlike PD155080, the ETB receptor-selective antagonist, RES-701 (1 microM), only prevented (P < 0.05) the inhibitory effect of endothelin-1 on the inward component of the IK(ACh); at -100 mV, RES-701, in the presence of endothelin-1 and acetylcholine, increased the "steady state" potassium current to -913 +/- 137 pA from -733 +/- 116 pA. Acetylcholine 177-180 endothelin-1 Oryctolagus cuniculus 130-142 8968552-19 1996 Unlike PD155080, the ETB receptor-selective antagonist, RES-701 (1 microM), only prevented (P < 0.05) the inhibitory effect of endothelin-1 on the inward component of the IK(ACh); at -100 mV, RES-701, in the presence of endothelin-1 and acetylcholine, increased the "steady state" potassium current to -913 +/- 137 pA from -733 +/- 116 pA. RES-701 195-202 endothelin-1 Oryctolagus cuniculus 130-142 8968552-19 1996 Unlike PD155080, the ETB receptor-selective antagonist, RES-701 (1 microM), only prevented (P < 0.05) the inhibitory effect of endothelin-1 on the inward component of the IK(ACh); at -100 mV, RES-701, in the presence of endothelin-1 and acetylcholine, increased the "steady state" potassium current to -913 +/- 137 pA from -733 +/- 116 pA. Acetylcholine 240-253 endothelin-1 Oryctolagus cuniculus 130-142 8968552-19 1996 Unlike PD155080, the ETB receptor-selective antagonist, RES-701 (1 microM), only prevented (P < 0.05) the inhibitory effect of endothelin-1 on the inward component of the IK(ACh); at -100 mV, RES-701, in the presence of endothelin-1 and acetylcholine, increased the "steady state" potassium current to -913 +/- 137 pA from -733 +/- 116 pA. Potassium 284-293 endothelin-1 Oryctolagus cuniculus 130-142 8968552-22 1996 In conclusion, endothelin-1 clearly inhibits the effects of acetylcholine on IK(ACh) in rabbit atrial cardiomyocytes. Acetylcholine 60-73 endothelin-1 Oryctolagus cuniculus 15-27 8968552-22 1996 In conclusion, endothelin-1 clearly inhibits the effects of acetylcholine on IK(ACh) in rabbit atrial cardiomyocytes. Acetylcholine 80-83 endothelin-1 Oryctolagus cuniculus 15-27 8968552-24 1996 Inhibition of the IK(ACh) may account for the positive chronotropic properties of endothelin-1. Acetylcholine 21-24 endothelin-1 Oryctolagus cuniculus 82-94 8841350-12 1996 Control vessels challenged with 3 nmol/L ET-1, which induced a magnitude of constriction similar to that of SAH (29%), relaxed in response to Ca(2+)-free solution, verapamil, and verapamil plus Ni2+ by 69%, 20%, and 50%, respectively (P > .05) versus respective values in SAH vessels). Verapamil 179-188 endothelin-1 Oryctolagus cuniculus 41-45 8841350-12 1996 Control vessels challenged with 3 nmol/L ET-1, which induced a magnitude of constriction similar to that of SAH (29%), relaxed in response to Ca(2+)-free solution, verapamil, and verapamil plus Ni2+ by 69%, 20%, and 50%, respectively (P > .05) versus respective values in SAH vessels). Nickel(2+) 194-198 endothelin-1 Oryctolagus cuniculus 41-45 8841350-13 1996 In contrast, control vessels challenged with 2 to 8 mumol/L serotonin, which induced a magnitude of constriction similar to those of SAH and ET-1 (22%), completely relaxed in response to Ca(2+)-free solution and verapamil. Serotonin 60-69 endothelin-1 Oryctolagus cuniculus 141-145 8938666-3 1996 The metalloprotease inhibitor phosphoramidon (30 mumol/l) almost abolished the contractile response to big ET-1, whereas the ET-1-induced contraction was unaffected. phosphoramidon 30-44 endothelin-1 Oryctolagus cuniculus 107-111 8938666-6 1996 The contractions induced by big ET-1, ET-1 and ET-3 were all inhibited by ET(A) receptor antagonist BQ 123 (3 mumol/l). cyclo(Trp-Asp-Pro-Val-Leu) 100-106 endothelin-1 Oryctolagus cuniculus 32-36 8938666-6 1996 The contractions induced by big ET-1, ET-1 and ET-3 were all inhibited by ET(A) receptor antagonist BQ 123 (3 mumol/l). cyclo(Trp-Asp-Pro-Val-Leu) 100-106 endothelin-1 Oryctolagus cuniculus 38-51 8938666-9 1996 These results suggest that externally applied big ET-1 is converted to ET-1 by a phosphoramidon-sensitive "endothelin converting enzyme" present in the vascular smooth muscle cells. phosphoramidon 81-95 endothelin-1 Oryctolagus cuniculus 50-54 8938666-9 1996 These results suggest that externally applied big ET-1 is converted to ET-1 by a phosphoramidon-sensitive "endothelin converting enzyme" present in the vascular smooth muscle cells. phosphoramidon 81-95 endothelin-1 Oryctolagus cuniculus 71-75 8841350-7 1996 Relaxation of SAH-, ET-1-, serotonin-, and KC1-constricted basilar artery in response to Ca(2+)-free solution, verapamil, and Ni2+ was measured in situ with the use of a cranial window. Verapamil 111-120 endothelin-1 Oryctolagus cuniculus 20-24 8841350-12 1996 Control vessels challenged with 3 nmol/L ET-1, which induced a magnitude of constriction similar to that of SAH (29%), relaxed in response to Ca(2+)-free solution, verapamil, and verapamil plus Ni2+ by 69%, 20%, and 50%, respectively (P > .05) versus respective values in SAH vessels). Verapamil 164-173 endothelin-1 Oryctolagus cuniculus 41-45 9389195-4 1996 It was found that ET-1 produced concentration- and time-dependent increases in 3H-TdR incorporation and in MAPK activity of these cells. Tritium 79-81 endothelin-1 Oryctolagus cuniculus 18-22 9389195-6 1996 Pre-treatment with PKC activator PMA (phorbol myristate acetate) for 24 h (PKC downregulation) significantly attenuated ET-1-induced MAPK activation. Tetradecanoylphorbol Acetate 33-36 endothelin-1 Oryctolagus cuniculus 120-124 9389195-6 1996 Pre-treatment with PKC activator PMA (phorbol myristate acetate) for 24 h (PKC downregulation) significantly attenuated ET-1-induced MAPK activation. Tetradecanoylphorbol Acetate 38-63 endothelin-1 Oryctolagus cuniculus 120-124 9389195-7 1996 These results indicate that: (1) ET-1-stimulated proliferation of VSMC involves the activation of MAPK and (2) ET-1-induced MAPK activation is mediated through ETA receptor and PKC. vsmc 66-70 endothelin-1 Oryctolagus cuniculus 33-37 9389195-7 1996 These results indicate that: (1) ET-1-stimulated proliferation of VSMC involves the activation of MAPK and (2) ET-1-induced MAPK activation is mediated through ETA receptor and PKC. vsmc 66-70 endothelin-1 Oryctolagus cuniculus 111-115 8818345-5 1996 BQ-788 (10 microM), an ETB receptor antagonist, inhibited responses produced by ET-3 (pKB = 5.1; n = 8), BQ-3020 (pKB = 5.2; n = 4) or S6c (pKB = 6.2; n = 9; P < 0.05 compared to potency versus ET-3- or BQ-3020-induced contractions), but was without inhibitory effect on ET-1-induced contractions (n = 5). BQ 788 0-6 endothelin-1 Oryctolagus cuniculus 274-278 8818345-8 1996 The combination of BQ-788 (10 microM) and BQ-123 (10 microM), an ETA-selective receptor antagonist, antagonized contractions produced by lower concentrations of ET-1 (1 and 3 nM) in rabbit pulmonary artery, but was without effect on responses elicited by higher concentrations of ET-1 (n = 5). BQ 788 19-25 endothelin-1 Oryctolagus cuniculus 161-165 8818345-8 1996 The combination of BQ-788 (10 microM) and BQ-123 (10 microM), an ETA-selective receptor antagonist, antagonized contractions produced by lower concentrations of ET-1 (1 and 3 nM) in rabbit pulmonary artery, but was without effect on responses elicited by higher concentrations of ET-1 (n = 5). BQ 788 19-25 endothelin-1 Oryctolagus cuniculus 280-284 8818345-8 1996 The combination of BQ-788 (10 microM) and BQ-123 (10 microM), an ETA-selective receptor antagonist, antagonized contractions produced by lower concentrations of ET-1 (1 and 3 nM) in rabbit pulmonary artery, but was without effect on responses elicited by higher concentrations of ET-1 (n = 5). cyclo(Trp-Asp-Pro-Val-Leu) 42-48 endothelin-1 Oryctolagus cuniculus 161-165 8818345-8 1996 The combination of BQ-788 (10 microM) and BQ-123 (10 microM), an ETA-selective receptor antagonist, antagonized contractions produced by lower concentrations of ET-1 (1 and 3 nM) in rabbit pulmonary artery, but was without effect on responses elicited by higher concentrations of ET-1 (n = 5). cyclo(Trp-Asp-Pro-Val-Leu) 42-48 endothelin-1 Oryctolagus cuniculus 280-284 8818345-9 1996 The combination of RES-701 (10 microM) and BQ-123 (10 microM) potentiated responses elicited by ET-1, producing a 3.7 fold shift to the left in the agonist concentration-response curve (n = 5). RES-701 19-26 endothelin-1 Oryctolagus cuniculus 96-100 8818345-9 1996 The combination of RES-701 (10 microM) and BQ-123 (10 microM) potentiated responses elicited by ET-1, producing a 3.7 fold shift to the left in the agonist concentration-response curve (n = 5). cyclo(Trp-Asp-Pro-Val-Leu) 43-49 endothelin-1 Oryctolagus cuniculus 96-100 8670786-11 1996 CONCLUSIONS: The higher concentrations of ET-LI in the tears and the lacrimal gland fluid than in plasma along with prepro ET-1 gene transcripts in the tear glands suggest that they secrete ET-1. et-li 42-47 endothelin-1 Oryctolagus cuniculus 190-194 8613968-8 1996 FR139317 at 10(-5) M decreased the ET-3-induced increase in inositol phosphates by about 60%, whereas it attenuated the increase in [3H]IP1 induced by ET-1 (3 x 10(-8)M) by only 20%. Tritium 133-135 endothelin-1 Oryctolagus cuniculus 151-155 8791007-5 1996 Endothelin-1 also decreased proteoglycan metabolism (about 50% of proteoglycan synthesis inhibition and 270 +/- 32% of degradation rate vs. basal, P < 0.05 in both cases) and enhanced total collagen (1.5 +/- 0.5 vs. 0.8 +/- 0.2 microgram hydroxyproline/microgram DNA in basal, P < 0.05) and fibronectin protein synthesis (157 +/- 14% of [35S] methionine incorporation vs. basal, P < 0.05). Hydroxyproline 241-255 endothelin-1 Oryctolagus cuniculus 0-12 8791007-5 1996 Endothelin-1 also decreased proteoglycan metabolism (about 50% of proteoglycan synthesis inhibition and 270 +/- 32% of degradation rate vs. basal, P < 0.05 in both cases) and enhanced total collagen (1.5 +/- 0.5 vs. 0.8 +/- 0.2 microgram hydroxyproline/microgram DNA in basal, P < 0.05) and fibronectin protein synthesis (157 +/- 14% of [35S] methionine incorporation vs. basal, P < 0.05). Sulfur-35 344-347 endothelin-1 Oryctolagus cuniculus 0-12 8791007-5 1996 Endothelin-1 also decreased proteoglycan metabolism (about 50% of proteoglycan synthesis inhibition and 270 +/- 32% of degradation rate vs. basal, P < 0.05 in both cases) and enhanced total collagen (1.5 +/- 0.5 vs. 0.8 +/- 0.2 microgram hydroxyproline/microgram DNA in basal, P < 0.05) and fibronectin protein synthesis (157 +/- 14% of [35S] methionine incorporation vs. basal, P < 0.05). Methionine 349-359 endothelin-1 Oryctolagus cuniculus 0-12 8791007-6 1996 The endothelin ETA receptor antagonist BQ-123 (Cyclo D-trp-D-asp-pro-D-val-leu) displaced [125I]endothelin-1 binding and inhibited endothelin-1 effects on extracellular matrix components. cyclo(Trp-Asp-Pro-Val-Leu) 39-45 endothelin-1 Oryctolagus cuniculus 96-108 8791007-6 1996 The endothelin ETA receptor antagonist BQ-123 (Cyclo D-trp-D-asp-pro-D-val-leu) displaced [125I]endothelin-1 binding and inhibited endothelin-1 effects on extracellular matrix components. cyclo(Trp-Asp-Pro-Val-Leu) 39-45 endothelin-1 Oryctolagus cuniculus 131-143 8791007-6 1996 The endothelin ETA receptor antagonist BQ-123 (Cyclo D-trp-D-asp-pro-D-val-leu) displaced [125I]endothelin-1 binding and inhibited endothelin-1 effects on extracellular matrix components. cyclo(Trp-Asp-Pro-Val-Leu) 47-78 endothelin-1 Oryctolagus cuniculus 96-108 8791007-6 1996 The endothelin ETA receptor antagonist BQ-123 (Cyclo D-trp-D-asp-pro-D-val-leu) displaced [125I]endothelin-1 binding and inhibited endothelin-1 effects on extracellular matrix components. cyclo(Trp-Asp-Pro-Val-Leu) 47-78 endothelin-1 Oryctolagus cuniculus 131-143 8791007-7 1996 The cell incubation with indomethacin totally reversed the endothelin-1 effect. Indomethacin 25-37 endothelin-1 Oryctolagus cuniculus 59-71 8613968-8 1996 FR139317 at 10(-5) M decreased the ET-3-induced increase in inositol phosphates by about 60%, whereas it attenuated the increase in [3H]IP1 induced by ET-1 (3 x 10(-8)M) by only 20%. Isopenicillin N 136-139 endothelin-1 Oryctolagus cuniculus 151-155 8613968-9 1996 Thus, in the presence of FR139317, the PIEs of ET-3 and ET-1 were partially dissociated from the PI hydrolysis that was induced by these isopeptides. FR 139317 25-33 endothelin-1 Oryctolagus cuniculus 56-60 8613968-10 1996 FR139317 inhibited the specific binding of [125I]ET-1 and of [125I]ET-3, and it was apparent that FR139317 had a high-affinity and a low-affinity site for competing for specific binding with each ligand. FR 139317 0-8 endothelin-1 Oryctolagus cuniculus 49-53 8882626-9 1996 The contractile responses to ET-1 and ET-2 were antagonized by pretreatment with BQ-123 (10 microM), but not at a concentration of 10 nM. cyclo(Trp-Asp-Pro-Val-Leu) 81-87 endothelin-1 Oryctolagus cuniculus 29-33 8818345-13 1996 RES-701 (3 microM) was without effect on contractions produced by S6c (n = 6) or BQ-3020 (n = 4), and potentiated rather than antagonized ET-1- or ET-3-induced responses (n = 6), reflected by a significant (about 6 fold) shift to the left in ET-1 or ET-3 concentration-response curves. RES-701 0-7 endothelin-1 Oryctolagus cuniculus 242-246 8818345-15 1996 The combination of RES-701 (3 microM) and BQ-123 (3 microM) potentiated responses elicited by ET-1, producing a 5.2 fold shift to the left in the agonist concentration-response curve (n = 5). RES-701 19-26 endothelin-1 Oryctolagus cuniculus 94-98 8818345-15 1996 The combination of RES-701 (3 microM) and BQ-123 (3 microM) potentiated responses elicited by ET-1, producing a 5.2 fold shift to the left in the agonist concentration-response curve (n = 5). cyclo(Trp-Asp-Pro-Val-Leu) 42-48 endothelin-1 Oryctolagus cuniculus 94-98 8882626-15 1996 ET-1 (100 nM) induced a transient increase in [Ca2+]i in a Ca(2+)-free, 2 mM EGTA-containing physiological saline solution (Ca(2+)-free PSS), and a small sustained contraction which was significantly different from that induced by ET-1 (100 nM) in normal PSS. Egtazic Acid 77-81 endothelin-1 Oryctolagus cuniculus 0-4 8882626-15 1996 ET-1 (100 nM) induced a transient increase in [Ca2+]i in a Ca(2+)-free, 2 mM EGTA-containing physiological saline solution (Ca(2+)-free PSS), and a small sustained contraction which was significantly different from that induced by ET-1 (100 nM) in normal PSS. Sodium Chloride 107-113 endothelin-1 Oryctolagus cuniculus 0-4 8882626-17 1996 The ET-1-induced transient increase in [Ca2+]i was significantly reduced by the sarcoplasmic reticulum (SR) Ca(2+)-ATPase inhibitor, cyclopiazonic acid (30 microM); however, the ET-1-induced sustained contraction was not affected by this agent. cyclopiazonic acid 133-151 endothelin-1 Oryctolagus cuniculus 4-8 8882626-17 1996 The ET-1-induced transient increase in [Ca2+]i was significantly reduced by the sarcoplasmic reticulum (SR) Ca(2+)-ATPase inhibitor, cyclopiazonic acid (30 microM); however, the ET-1-induced sustained contraction was not affected by this agent. cyclopiazonic acid 133-151 endothelin-1 Oryctolagus cuniculus 178-182 8882626-22 1996 The selective ETB receptor antagonist, BQ-788 (1 microM) reduced the transient increase in [Ca2+]i induced by ET-1 (30 nM), ET-2 (30 nM), ET-3 (100 nM) and IRL1620 (1 microM), but did not affect the sustained elevation of [Ca2+]i and contractile responses produced by ET-1, ET-2 and ET-3. BQ 788 39-45 endothelin-1 Oryctolagus cuniculus 110-114 8882626-22 1996 The selective ETB receptor antagonist, BQ-788 (1 microM) reduced the transient increase in [Ca2+]i induced by ET-1 (30 nM), ET-2 (30 nM), ET-3 (100 nM) and IRL1620 (1 microM), but did not affect the sustained elevation of [Ca2+]i and contractile responses produced by ET-1, ET-2 and ET-3. BQ 788 39-45 endothelin-1 Oryctolagus cuniculus 268-272 9011641-7 1996 Perfusion with either 10 PM ET-1 at constant coronary artery flow for 5 min in lieu of ischemia or 50 PM ET-1 with 10 nM nicardipine to block the former"s coronary constructive effect was quite protective and equipotent with preconditioning. Nicardipine 121-132 endothelin-1 Oryctolagus cuniculus 105-109 8772525-5 1996 Extracellular calcium influx via voltage-dependent channels is necessary for contraction to ET-1 in rabbit and dog airways. Calcium 14-21 endothelin-1 Oryctolagus cuniculus 92-96 8758691-3 1996 (2) The decreases in RPF and VDD induced by ET-1 could be virtually blocked by pretreatment with ET(A) receptor blocker BQ-123 (20 or 100 micrograms/L). cyclo(Trp-Asp-Pro-Val-Leu) 120-126 endothelin-1 Oryctolagus cuniculus 44-48 8758691-5 1996 (3) The negative chronotropic action of ET-1 on the pacemaker cells was totally abolished by pretreatment with a kind of KATP channel blocker glibenclamide (10 mumol/L). Glyburide 142-155 endothelin-1 Oryctolagus cuniculus 40-44 8720476-2 1996 Methoxamine by itself elicited a positive inotropic effect and it simultaneously inhibited the positive inotropic effects of endothelin-1 and endothelin-3 without affecting the acceleration of the hydrolysis of phosphoinositide that was induced by the endothelin isopeptides. Methoxamine 0-11 endothelin-1 Oryctolagus cuniculus 125-137 7503068-7 1995 BSA and fatty acid-free BSA stimulated tubular ET-1 synthesis and release to a comparable extent, indicating that the lipid component of the molecule is not involved in the observed phenomenon. Fatty Acids 8-18 endothelin-1 Oryctolagus cuniculus 47-51 8739497-0 1996 Effects of BQ-123, an ETA recepter-selective antagonist, on changes of intraocular pressure, blood-aqueous barrier and aqueous prostaglandin concentrations caused by endothelin-1 in rabbit. cyclo(Trp-Asp-Pro-Val-Leu) 11-17 endothelin-1 Oryctolagus cuniculus 166-178 8739497-0 1996 Effects of BQ-123, an ETA recepter-selective antagonist, on changes of intraocular pressure, blood-aqueous barrier and aqueous prostaglandin concentrations caused by endothelin-1 in rabbit. Prostaglandins 127-140 endothelin-1 Oryctolagus cuniculus 166-178 8739497-13 1996 When used in combination with ET-1, BQ-123 (126.5 micrograms) significantly inhibited both the IOP rise (0.5-1 hour) and the reduction (24-72 hours) caused by ET-1 (0.5 microgram). cyclo(Trp-Asp-Pro-Val-Leu) 36-42 endothelin-1 Oryctolagus cuniculus 30-34 8739497-13 1996 When used in combination with ET-1, BQ-123 (126.5 micrograms) significantly inhibited both the IOP rise (0.5-1 hour) and the reduction (24-72 hours) caused by ET-1 (0.5 microgram). cyclo(Trp-Asp-Pro-Val-Leu) 36-42 endothelin-1 Oryctolagus cuniculus 159-163 8739497-14 1996 BQ-123 (12.6 micrograms) also significantly inhibited the IOP reduction (6-8 hours and 72-96 hours) caused by ET-1 (0.05 micrograms). cyclo(Trp-Asp-Pro-Val-Leu) 0-6 endothelin-1 Oryctolagus cuniculus 110-114 8739497-16 1996 The IOP response and the elevation of aqueous protein and PGE2 concentration following ET-1 injection are at least partly mediated by ETA receptors. Dinoprostone 58-62 endothelin-1 Oryctolagus cuniculus 87-91 8531067-12 1995 These results suggest that ET-1 enhances nervous acetylcholine release by simultaneously activating the ET-1-selective ETA receptor and the isopeptide-nonselective ETB receptor (ETB1 subtype that is sensitive to both RES-701-1 and BQ-788 and the ETB2 subtype that is sensitive only to BQ-788). Acetylcholine 49-62 endothelin-1 Oryctolagus cuniculus 27-31 7575594-2 1995 Thus, the effect of endothelin-1 on the stimulation of tyrosine phosphorylation in rabbit platelets can be inhibited by preincubation with forskolin, which increase the cAMP level. Tyrosine 55-63 endothelin-1 Oryctolagus cuniculus 20-32 8605959-3 1995 However, endothelin-1-induced contractions were much less sensitive to an endothelin ETA receptor antagonist, BQ-123 (cyclo (-D-Asp-L-Pro-D-Val-L-Leu-D-Trp-)), than sarafotoxin S6b-induced responses. cyclo(Trp-Asp-Pro-Val-Leu) 110-116 endothelin-1 Oryctolagus cuniculus 9-21 8605959-3 1995 However, endothelin-1-induced contractions were much less sensitive to an endothelin ETA receptor antagonist, BQ-123 (cyclo (-D-Asp-L-Pro-D-Val-L-Leu-D-Trp-)), than sarafotoxin S6b-induced responses. cyclo (-d-asp-l-pro-d-val-l-leu-d-trp 118-155 endothelin-1 Oryctolagus cuniculus 9-21 8605959-3 1995 However, endothelin-1-induced contractions were much less sensitive to an endothelin ETA receptor antagonist, BQ-123 (cyclo (-D-Asp-L-Pro-D-Val-L-Leu-D-Trp-)), than sarafotoxin S6b-induced responses. sarafotoxin 165-176 endothelin-1 Oryctolagus cuniculus 9-21 8605959-3 1995 However, endothelin-1-induced contractions were much less sensitive to an endothelin ETA receptor antagonist, BQ-123 (cyclo (-D-Asp-L-Pro-D-Val-L-Leu-D-Trp-)), than sarafotoxin S6b-induced responses. AKOS012388270 177-180 endothelin-1 Oryctolagus cuniculus 9-21 8605959-4 1995 The pA2 values of BQ-123 for endothelin-1- and sarafotoxin S6b-induced contractions were 5.69 and 7.65, respectively. cyclo(Trp-Asp-Pro-Val-Leu) 18-24 endothelin-1 Oryctolagus cuniculus 29-41 8590976-10 1995 The reduction in infarct size afforded by ET-1 (0.03 nmol kg-1) was abolished by pretreatment of rabbits with the KATP channel inhibitors, glibenclamide (0.3 mg kg-1) and 5-HD (5 mg kg-1), (infarct size 59 +/- 3 and 63 +/- 4% respectively; n = 4-9). Glyburide 139-152 endothelin-1 Oryctolagus cuniculus 42-46 8751073-5 1995 A selective ETA receptor antagonist, BQ 123, and selective ETB agonists, ET-3 and sarafotoxin S6c (STXc), inhibited [125I]ET-1 binding to bladder dome, bladder base, and urethra with high and low affinity constants indicating the presence of both ETA and ETB receptor subtypes in these tissues. bulaquine 37-39 endothelin-1 Oryctolagus cuniculus 122-126 8751073-5 1995 A selective ETA receptor antagonist, BQ 123, and selective ETB agonists, ET-3 and sarafotoxin S6c (STXc), inhibited [125I]ET-1 binding to bladder dome, bladder base, and urethra with high and low affinity constants indicating the presence of both ETA and ETB receptor subtypes in these tissues. sarafotoxin 82-93 endothelin-1 Oryctolagus cuniculus 122-126 7575594-2 1995 Thus, the effect of endothelin-1 on the stimulation of tyrosine phosphorylation in rabbit platelets can be inhibited by preincubation with forskolin, which increase the cAMP level. Colforsin 139-148 endothelin-1 Oryctolagus cuniculus 20-32 7589224-7 1995 The area with increased binding of [125I]endothelin-1 corresponded to the zone of arterial injury stained with Evans blue. Evans Blue 111-121 endothelin-1 Oryctolagus cuniculus 41-53 8531067-12 1995 These results suggest that ET-1 enhances nervous acetylcholine release by simultaneously activating the ET-1-selective ETA receptor and the isopeptide-nonselective ETB receptor (ETB1 subtype that is sensitive to both RES-701-1 and BQ-788 and the ETB2 subtype that is sensitive only to BQ-788). Acetylcholine 49-62 endothelin-1 Oryctolagus cuniculus 104-108 8531067-12 1995 These results suggest that ET-1 enhances nervous acetylcholine release by simultaneously activating the ET-1-selective ETA receptor and the isopeptide-nonselective ETB receptor (ETB1 subtype that is sensitive to both RES-701-1 and BQ-788 and the ETB2 subtype that is sensitive only to BQ-788). bulaquine 231-233 endothelin-1 Oryctolagus cuniculus 27-31 7573496-6 1995 ACh in situ and in vitro relaxed endothelin-1 (ET-1)-contracted control vessels to a smaller magnitude than 5-HT-contracted control vessels. Acetylcholine 0-3 endothelin-1 Oryctolagus cuniculus 33-45 7573496-6 1995 ACh in situ and in vitro relaxed endothelin-1 (ET-1)-contracted control vessels to a smaller magnitude than 5-HT-contracted control vessels. Acetylcholine 0-3 endothelin-1 Oryctolagus cuniculus 47-51 7592055-8 1995 Members of this series of compounds demonstrated antagonist behavior in a secondary assay based on blockade of ET-1 stimulated arachidonic acid release from rabbit renal artery smooth muscle cells, when present at concentrations of > or = 30 microM. Arachidonic Acid 127-143 endothelin-1 Oryctolagus cuniculus 111-115 7550073-0 1995 Demonstration of endothelin (ET) receptors on cultured rabbit chondrocytes and stimulation of DNA synthesis and calcium influx by ET-1 via its receptors. Calcium 112-119 endothelin-1 Oryctolagus cuniculus 130-134 7550073-2 1995 After crosslinking the receptors on the cells with 125I-ET-1, two major bands of 43 kDa and 46 kDa were separated by SDS-PAGE. Sodium Dodecyl Sulfate 117-120 endothelin-1 Oryctolagus cuniculus 56-60 7550073-4 1995 The binding of ET-1 to chondrocytes was increased by treatment with PTH, DBcAMP, TGF-beta 1, IL-1 beta, RA and EGF. Bucladesine 73-79 endothelin-1 Oryctolagus cuniculus 15-19 7550073-6 1995 ET-1 also stimulated calcium incorporation through the cell membrane of chondrocytes. Calcium 21-28 endothelin-1 Oryctolagus cuniculus 0-4 7557507-3 1995 METHODS: We measured CBF in a rabbit model of transient complete obstruction of retinal vessels induced by intravitreal injection of a high dose of ET-1, using the hydrogen clearance method. Hydrogen 164-172 endothelin-1 Oryctolagus cuniculus 148-152 8642900-16 1995 FLU reduced the contractile effects of ET-1 (1 x 10(-12)M-1 x 10(-8)M). Flunarizine 0-3 endothelin-1 Oryctolagus cuniculus 39-43 7582460-7 1995 The sustained elevation induced by 10(-10) M ET-1 was blocked by 300 microM mefenamic acid (a cation channel blocker) but not by 10 microM nifedipine (a blocker of voltage-operated Ca2+ channel). Mefenamic Acid 76-90 endothelin-1 Oryctolagus cuniculus 45-49 7582460-11 1995 The current was reversibly blocked by 300 microM mefenamic acid, and it persisted after all cations in the bath solution had been replaced by Ca2+ (5 or 30 mM) and nonpermeant cation N-methyl-D glucamine,indicating that the ET-1-activated channel is permeable to Ca2+. Mefenamic Acid 49-63 endothelin-1 Oryctolagus cuniculus 224-228 7582460-11 1995 The current was reversibly blocked by 300 microM mefenamic acid, and it persisted after all cations in the bath solution had been replaced by Ca2+ (5 or 30 mM) and nonpermeant cation N-methyl-D glucamine,indicating that the ET-1-activated channel is permeable to Ca2+. n-methyl-d glucamine 183-203 endothelin-1 Oryctolagus cuniculus 224-228 7582460-16 1995 The current was reversibly blocked by 300 microM mefenamic acid and was permeable to Ca2+,showing marked similarities to ET-1-induced cationic current in Ltk- cells.5. Mefenamic Acid 49-63 endothelin-1 Oryctolagus cuniculus 121-125 7610997-6 1995 These results suggest that ET-1 is an important mediator in the ocular inflammatory reactions through the arachidonic acid cascade. Arachidonic Acid 106-122 endothelin-1 Oryctolagus cuniculus 27-31 7671630-5 1995 A masked, randomized, study revealed that the intraperitoneal administration of indomethacin (50 mg/kg) prior to ET-1 injection significantly reduced the ocular hypertensive response, but not th ocular hypotensive response, to ET-1. Indomethacin 80-92 endothelin-1 Oryctolagus cuniculus 227-231 7780649-4 1995 Binding of [125I]-ET-1 to ET receptors on rabbit ventricular and cerebellar membrane fractions was inhibited by TAK-044 with IC50 values of 3.8 nM and 130 nM, respectively. TAK 044 112-119 endothelin-1 Oryctolagus cuniculus 18-22 7539147-0 1995 Evidence for a role of endothelin 1 and protein kinase C in nitroglycerin tolerance. Nitroglycerin 60-73 endothelin-1 Oryctolagus cuniculus 23-35 7539147-5 1995 Paradoxically, constrictions caused by endothelin 1 were decreased in nitrate-tolerant vessels. Nitrates 70-77 endothelin-1 Oryctolagus cuniculus 39-51 7539147-6 1995 Immunocytochemical analysis revealed intense endothelin 1-like and big endothelin 1-like immunoreactivity in the media of nitroglycerin-tolerant but not of control aortas. Nitroglycerin 122-135 endothelin-1 Oryctolagus cuniculus 71-83 7539147-7 1995 The enhanced vasoconstriction to angiotensin II, serotonin, KCl, and phenylephrine could be mimicked in normal vessels by addition of subthreshold concentrations of endothelin 1, and this effect was prevented by calphostin C. Serotonin 49-58 endothelin-1 Oryctolagus cuniculus 165-177 7539147-7 1995 The enhanced vasoconstriction to angiotensin II, serotonin, KCl, and phenylephrine could be mimicked in normal vessels by addition of subthreshold concentrations of endothelin 1, and this effect was prevented by calphostin C. Potassium Chloride 60-63 endothelin-1 Oryctolagus cuniculus 165-177 7539147-7 1995 The enhanced vasoconstriction to angiotensin II, serotonin, KCl, and phenylephrine could be mimicked in normal vessels by addition of subthreshold concentrations of endothelin 1, and this effect was prevented by calphostin C. Phenylephrine 69-82 endothelin-1 Oryctolagus cuniculus 165-177 7539147-7 1995 The enhanced vasoconstriction to angiotensin II, serotonin, KCl, and phenylephrine could be mimicked in normal vessels by addition of subthreshold concentrations of endothelin 1, and this effect was prevented by calphostin C. calphostin C 212-224 endothelin-1 Oryctolagus cuniculus 165-177 7539147-8 1995 We propose that increased autocrine production of endothelin 1 in nitrate tolerance sensitizes vascular smooth muscle to a variety of vasoconstrictors through a protein kinase C-mediated mechanism. Nitrates 66-73 endothelin-1 Oryctolagus cuniculus 50-62 7630158-4 1995 [125I]ET-1 binding was completely inhibited by unlabeled ET-1 and Ro 46-2005, a mixed-type antagonist for the ETA and ETB receptors, but partially by BQ123, a selective antagonist for ETA receptors, and IRL1620. cyclo(Trp-Asp-Pro-Val-Leu) 150-155 endothelin-1 Oryctolagus cuniculus 6-10 7630158-8 1995 The histochemical experiments with biotinylated ET-1 at lysine-9 side chain alone or in combination with unlabeled ET-1, BQ123, Ro 46-2005, or IRL1620, showed the ETA receptors to be localized mainly in the media, whereas the ETB receptors localized mainly in the neointima. Lysine 56-62 endothelin-1 Oryctolagus cuniculus 48-52 8587372-2 1995 Cooling has been shown to decrease the sensitivity of cutaneous rabbit ear arteries to endothelin-1 (ET-1), perhaps by increasing the availability of nitric oxide (NO). Nitric Oxide 150-162 endothelin-1 Oryctolagus cuniculus 87-99 7732916-5 1995 Aqueous prostaglandin (PG) E2, determined by radioimmunoassay, was significantly elevated following intravitreal injection of ET-1 (10(-5)M). Dinoprostone 8-29 endothelin-1 Oryctolagus cuniculus 126-130 7732916-6 1995 Prior injection of 97-139 (10(-2)M) significantly suppressed the elevation of aqueous PGE2 concentration caused by ET-1 (10(-5)M). Dinoprostone 86-90 endothelin-1 Oryctolagus cuniculus 115-119 7732916-7 1995 These results suggest that both the IOP response and the elevation of aqueous PGE2 following ET-1 injection are at least partially mediated by ETA receptor. Dinoprostone 78-82 endothelin-1 Oryctolagus cuniculus 93-97 7890245-6 1995 Water content was increased in brain mass in ET-1-treated rabbits (p = 0.001) suggesting an ET-1-induced edema. Water 0-5 endothelin-1 Oryctolagus cuniculus 45-49 7890245-6 1995 Water content was increased in brain mass in ET-1-treated rabbits (p = 0.001) suggesting an ET-1-induced edema. Water 0-5 endothelin-1 Oryctolagus cuniculus 92-96 7759212-12 1995 The effect of adding CaCl2 was inhibited in the buffer with nifedipine and in iohexol when the vessels were activated with histamine, endothelin-1, or PGF2 alpha. Calcium Chloride 21-26 endothelin-1 Oryctolagus cuniculus 134-146 7759212-12 1995 The effect of adding CaCl2 was inhibited in the buffer with nifedipine and in iohexol when the vessels were activated with histamine, endothelin-1, or PGF2 alpha. Iohexol 78-85 endothelin-1 Oryctolagus cuniculus 134-146 8587372-2 1995 Cooling has been shown to decrease the sensitivity of cutaneous rabbit ear arteries to endothelin-1 (ET-1), perhaps by increasing the availability of nitric oxide (NO). Nitric Oxide 150-162 endothelin-1 Oryctolagus cuniculus 101-105 8587395-5 1995 ET-1-induced contractions and elevations of [Ca]i were blocked by the dual L- and R-type calcium-channel blocker (-)PN200 110 (10(-6) M), whereas nifedipine (10(-6) M) affected only the latter parameter. Nifedipine 146-156 endothelin-1 Oryctolagus cuniculus 0-4 8587395-6 1995 BQ-123, a selective ET(A) receptor antagonist, almost totally blocked the ET-1-induced contraction and elevation of [Ca]i. cyclo(Trp-Asp-Pro-Val-Leu) 0-6 endothelin-1 Oryctolagus cuniculus 74-78 8587396-1 1995 Endothelin-1 (ET-1) induced a concentration-dependent sustained increase of intracellular calcium ([Ca2+]i) in single cells of rabbit aortic vascular smooth-muscle cells (VSMCs). Calcium 90-97 endothelin-1 Oryctolagus cuniculus 0-12 8587396-1 1995 Endothelin-1 (ET-1) induced a concentration-dependent sustained increase of intracellular calcium ([Ca2+]i) in single cells of rabbit aortic vascular smooth-muscle cells (VSMCs). Calcium 90-97 endothelin-1 Oryctolagus cuniculus 14-18 8587396-6 1995 Our results suggest that the sustained [Ca2+]i increase induced by ET-1, as with insulin, was mainly due to activation of a nifedipine-insensitive but isradipine-sensitive steady-state, voltage-dependent R-type calcium channel via an as yet unidentified ET receptor. Nifedipine 124-134 endothelin-1 Oryctolagus cuniculus 67-71 8587396-6 1995 Our results suggest that the sustained [Ca2+]i increase induced by ET-1, as with insulin, was mainly due to activation of a nifedipine-insensitive but isradipine-sensitive steady-state, voltage-dependent R-type calcium channel via an as yet unidentified ET receptor. Isradipine 151-161 endothelin-1 Oryctolagus cuniculus 67-71 8587398-4 1995 PD 156707, however, produced monophasic inhibitions of the ET-1-induced dose-response curve of rabbit aortas pretreated either with BQ 788 (an ETB-selective antagonist) or desensitized with sarafotoxin S6c (an ETB-selective agonist). s6c 202-205 endothelin-1 Oryctolagus cuniculus 59-63 8587398-4 1995 PD 156707, however, produced monophasic inhibitions of the ET-1-induced dose-response curve of rabbit aortas pretreated either with BQ 788 (an ETB-selective antagonist) or desensitized with sarafotoxin S6c (an ETB-selective agonist). PD 156707 0-9 endothelin-1 Oryctolagus cuniculus 59-63 8587398-4 1995 PD 156707, however, produced monophasic inhibitions of the ET-1-induced dose-response curve of rabbit aortas pretreated either with BQ 788 (an ETB-selective antagonist) or desensitized with sarafotoxin S6c (an ETB-selective agonist). BQ 788 132-138 endothelin-1 Oryctolagus cuniculus 59-63 8587398-4 1995 PD 156707, however, produced monophasic inhibitions of the ET-1-induced dose-response curve of rabbit aortas pretreated either with BQ 788 (an ETB-selective antagonist) or desensitized with sarafotoxin S6c (an ETB-selective agonist). sarafotoxin 190-201 endothelin-1 Oryctolagus cuniculus 59-63 8587417-3 1995 Therefore, a 15-min infusion of BQ-123 (1 microM) prevented the vasoconstrictor response to ET-1 (10 nM). cyclo(Trp-Asp-Pro-Val-Leu) 32-38 endothelin-1 Oryctolagus cuniculus 92-96 8587406-6 1995 Emulsion-dipped slides of sections of rabbit eyes incubated with [125I]ET-1 showed specific labeling for binding sites over structures that were identified from serial sections stained with hematoxylineosin. hematoxylineosin 190-206 endothelin-1 Oryctolagus cuniculus 71-75 8587417-7 1995 Co-infusion of supramaximal concentrations of BQ-123 (1 microM) and BQ-788 (10 nM) still reduced by about 90% the vasoconstrictor response to ET-1. cyclo(Trp-Asp-Pro-Val-Leu) 46-52 endothelin-1 Oryctolagus cuniculus 142-146 8587417-7 1995 Co-infusion of supramaximal concentrations of BQ-123 (1 microM) and BQ-788 (10 nM) still reduced by about 90% the vasoconstrictor response to ET-1. BQ 788 68-74 endothelin-1 Oryctolagus cuniculus 142-146 8587417-9 1995 Similarly, a mixture of both antagonists was less effective than BQ-123 alone in reducing the pressor effect of ET-1 in anesthetized rabbits. cyclo(Trp-Asp-Pro-Val-Leu) 65-71 endothelin-1 Oryctolagus cuniculus 112-116 8587417-12 1995 These results suggest that the systemic and renal vasculatures would respond to ET-1 with enhanced vasoconstriction after treatment with mixed ETA/ETB receptor antagonists owing to the slow reversibility of the antagonism of the ETB receptor-mediated release of nitric oxide. Nitric Oxide 262-274 endothelin-1 Oryctolagus cuniculus 80-84 8587477-1 1995 We had earlier shown that endothelin-1 (ET-1)-induced vasoconstriction and prostacyclin (PGI2) release in the rabbit kidney are exclusively linked to ETA receptor activation. Epoprostenol 75-87 endothelin-1 Oryctolagus cuniculus 40-44 8577073-10 1995 The aqueous prostaglandin E2 concentration was significantly increased at both 1 and 24 hours after 10(-5) M ET-1 injection. Dinoprostone 12-28 endothelin-1 Oryctolagus cuniculus 109-113 8926643-1 1995 The authors have reported that ET-1 and ETB receptor agonist, sarafotoxin S6c (STX-S6c), caused prolonged intraocular pressure (IOP) reduction in rabbit eyes in a dose-dependent fashion when injected intravitreally. Saxitoxin 79-82 endothelin-1 Oryctolagus cuniculus 31-52 7887328-0 1995 [Effect of systemic calcium antagonist on a model of ocular circulation disturbance induced by endothelin-1]. Calcium 20-27 endothelin-1 Oryctolagus cuniculus 95-107 7887328-1 1995 We studied the effect of generally applied calcium antagonist on endothelin (ET-1) treated rabbit eyes. Calcium 43-50 endothelin-1 Oryctolagus cuniculus 77-81 7887328-3 1995 In the control group (0.4 ml/kg of saline), administration of ET-1 caused contraction of the retinal artery, decrease of the capillary blood flow in ONH, prolongation of the VEP latency, and reduction of IOP. Sodium Chloride 35-41 endothelin-1 Oryctolagus cuniculus 62-66 7887328-5 1995 These results showed that generally applied calcium antagonist inhibits the disturbance of ocular circulation induced by ET-1. Calcium 44-51 endothelin-1 Oryctolagus cuniculus 121-125 8587477-2 1995 In contrast, another study showed that ET-1 inhibited platelet aggregation ex vivo through the release of PGI2 solely via the activation of ETB receptors. Epoprostenol 106-110 endothelin-1 Oryctolagus cuniculus 39-43 8587477-3 1995 In an attempt to identify the organs involved in the ET-1-induced release of PGI2 in vivo, we characterized the receptors responsible for the release of this eicosanoid and also monitored the activity of the endothelin-converting enzyme (ECE) in rabbit pulmonary lobe. Epoprostenol 77-81 endothelin-1 Oryctolagus cuniculus 53-57 8587477-3 1995 In an attempt to identify the organs involved in the ET-1-induced release of PGI2 in vivo, we characterized the receptors responsible for the release of this eicosanoid and also monitored the activity of the endothelin-converting enzyme (ECE) in rabbit pulmonary lobe. Eicosanoids 158-168 endothelin-1 Oryctolagus cuniculus 53-57 8587477-4 1995 In this perfused organ, a 5-min infusion of ET-1 (50 nM) triggered a marked release of PGI2 and an increase in perfusion pressure, both of which were mimicked by the selective ETB agonist IRL 1620 (0.5 microM). Epoprostenol 87-91 endothelin-1 Oryctolagus cuniculus 44-48 8587477-5 1995 Furthermore, a selective ETB antagonist, BQ-788 (10 nM), markedly blunted the release of PGI2 induced by ET-1 (50 nM). BQ 788 41-47 endothelin-1 Oryctolagus cuniculus 105-109 8587477-5 1995 Furthermore, a selective ETB antagonist, BQ-788 (10 nM), markedly blunted the release of PGI2 induced by ET-1 (50 nM). Epoprostenol 89-93 endothelin-1 Oryctolagus cuniculus 105-109 7996430-0 1994 Inhibition by nickel of endothelin-1-induced tension and associated 45Ca movements in rabbit aorta. Nickel 14-20 endothelin-1 Oryctolagus cuniculus 24-36 7889281-10 1994 In the rabbit perfused kidney, BQ-788 (10 nM) potentiated the increase of perfusion pressure induced by endothelin-1 (1, 5 and 10 nM) by approximately 90%, but not that induced by angiotensin II (1 microM). BQ 788 31-37 endothelin-1 Oryctolagus cuniculus 104-116 7889281-13 1994 In another series of experiments, pretreatment of the perfused kidney with a nitric oxide synthase inhibitor, L-NAME (100 microM), potentiated the pressor responses to both endothelin-1 and angiotensin II. NG-Nitroarginine Methyl Ester 110-116 endothelin-1 Oryctolagus cuniculus 173-185 7867036-0 1994 Phosphatidic acid increases in response to noradrenaline and endothelin-1 in adult rabbit ventricular myocytes. Phosphatidic Acids 0-17 endothelin-1 Oryctolagus cuniculus 61-73 7867036-1 1994 OBJECTIVE: The aim was to assess whether noradrenaline and endothelin-1 can stimulate endogenous production of phosphatidic acid in adult ventricular myocytes. Phosphatidic Acids 111-128 endothelin-1 Oryctolagus cuniculus 59-71 7867036-6 1994 An increase in phosphatidic acid was also elicited in rabbit ventricular myocytes in response to endothelin-1. Phosphatidic Acids 15-32 endothelin-1 Oryctolagus cuniculus 97-109 7867036-7 1994 The response was time and concentration dependent with the maximal increase at 12 min, EC50 5.3 x 10(-9) M, and maximum effect at 10(-6) M. Both noradrenalin and endothelin-1 stimulated phosphatidylbutanol production in the presence of butanol (100 mM), indicating that both agonists activate phospholipase D. CONCLUSIONS: Noradrenaline at physiological concentrations elicits both a rapid and a delayed increase in phosphatidic acid in adult rabbit ventricular myocytes. Butanols 198-205 endothelin-1 Oryctolagus cuniculus 162-174 7867036-7 1994 The response was time and concentration dependent with the maximal increase at 12 min, EC50 5.3 x 10(-9) M, and maximum effect at 10(-6) M. Both noradrenalin and endothelin-1 stimulated phosphatidylbutanol production in the presence of butanol (100 mM), indicating that both agonists activate phospholipase D. CONCLUSIONS: Noradrenaline at physiological concentrations elicits both a rapid and a delayed increase in phosphatidic acid in adult rabbit ventricular myocytes. Norepinephrine 323-336 endothelin-1 Oryctolagus cuniculus 162-174 7867036-7 1994 The response was time and concentration dependent with the maximal increase at 12 min, EC50 5.3 x 10(-9) M, and maximum effect at 10(-6) M. Both noradrenalin and endothelin-1 stimulated phosphatidylbutanol production in the presence of butanol (100 mM), indicating that both agonists activate phospholipase D. CONCLUSIONS: Noradrenaline at physiological concentrations elicits both a rapid and a delayed increase in phosphatidic acid in adult rabbit ventricular myocytes. Phosphatidic Acids 416-433 endothelin-1 Oryctolagus cuniculus 162-174 7867036-9 1994 Activation of phospholipase D contributes to the increase in phosphatidic acid seen during stimulation of myocytes with either noradrenaline or endothelin-1. Phosphatidic Acids 61-78 endothelin-1 Oryctolagus cuniculus 144-156 7996447-2 1994 In the endothelium loaded with fura-2, ET-1 (1-100 nM) induced large transient increase followed by small sustained increase in cytosolic Ca++ level ([Ca++]i) in a concentration-dependent manner. Fura-2 31-37 endothelin-1 Oryctolagus cuniculus 39-43 7996447-3 1994 ET-3 induced only a small increase in [Ca++]i at higher concentrations (100-300 nM) than ET-1, whereas a selective ETB agonist, 100 nM IRL 1620 (succinyl-[Glu9, Ala11,15]ET-1 8-21), was ineffective. succinyl-[glu9 145-159 endothelin-1 Oryctolagus cuniculus 170-174 7996447-6 1994 In the absence of external Ca++ (with 0.5 mM EGTA), ET-1 induced only a transient increase in [Ca++]i, which was inhibited by an inhibitor of Ca+(+)-ATPase in endoplasmic reticulum, 1 microM thapsigargin. Egtazic Acid 45-49 endothelin-1 Oryctolagus cuniculus 52-56 7996447-6 1994 In the absence of external Ca++ (with 0.5 mM EGTA), ET-1 induced only a transient increase in [Ca++]i, which was inhibited by an inhibitor of Ca+(+)-ATPase in endoplasmic reticulum, 1 microM thapsigargin. Thapsigargin 191-203 endothelin-1 Oryctolagus cuniculus 52-56 7996430-1 1994 Contractions induced by 10 nM endothelin-1 (ET) in the rabbit aortic media intimal layer were inhibited by prior exposure to 100 microM Ni++ (33.1%) or to a Ca(++)-free buffer (80.2%) but were unaffected by pretreatment with 0.1 microM nifedipine. Nifedipine 236-246 endothelin-1 Oryctolagus cuniculus 30-42 7996430-1 1994 Contractions induced by 10 nM endothelin-1 (ET) in the rabbit aortic media intimal layer were inhibited by prior exposure to 100 microM Ni++ (33.1%) or to a Ca(++)-free buffer (80.2%) but were unaffected by pretreatment with 0.1 microM nifedipine. Nifedipine 236-246 endothelin-1 Oryctolagus cuniculus 44-46 7896062-2 1994 Endothelin-1 (21-amino acids) and pre-pro endothelin-1 (39-amino acids) produced a concentration-dependent increase in perfusion pressure when infused through the renal artery of rabbit isolated perfused kidney. 21-amino acids 14-28 endothelin-1 Oryctolagus cuniculus 0-12 7896062-7 1994 Methylene blue in the medium caused a highly significant potentiation in the vasoconstrictor response to endothelin-1. Methylene Blue 0-14 endothelin-1 Oryctolagus cuniculus 105-117 7896062-3 1994 Addition of phosphoramidon to the medium caused a potentiation in the vasoconstrictor response to endothelin-1 and greatly, but not completely, inhibited vasoconstriction induced by pre-pro-endothelin-1. phosphoramidon 12-26 endothelin-1 Oryctolagus cuniculus 98-110 7896062-9 1994 A short-term infusion of endothelin-1 through the renal artery elicited a decrease in urine flow which returned to control levels after perfusing the kidney with Krebs buffer, but prolonged infusion of the peptide produced an irreversible increase in urine flow. krebs 162-167 endothelin-1 Oryctolagus cuniculus 25-37 7896062-3 1994 Addition of phosphoramidon to the medium caused a potentiation in the vasoconstrictor response to endothelin-1 and greatly, but not completely, inhibited vasoconstriction induced by pre-pro-endothelin-1. phosphoramidon 12-26 endothelin-1 Oryctolagus cuniculus 190-202 7896062-13 1994 From these results it was concluded that phosphoramidon-sensitive endothelin-converting enzyme, probably localized in microvasculature of the kidney, can convert pre-pro-endothelin-1 to endothelin-1 which is responsible for the vasoconstrictor effect of pre-pro-endothelin-1 in addition to its possible direct vasoconstrictor effect on kidney vasculature. phosphoramidon 41-55 endothelin-1 Oryctolagus cuniculus 170-182 7834180-0 1994 Synergistic inhibition by BQ-123 and BQ-788 of endothelin-1-induced contractions of the rabbit pulmonary artery. cyclo(Trp-Asp-Pro-Val-Leu) 26-32 endothelin-1 Oryctolagus cuniculus 47-59 7896062-13 1994 From these results it was concluded that phosphoramidon-sensitive endothelin-converting enzyme, probably localized in microvasculature of the kidney, can convert pre-pro-endothelin-1 to endothelin-1 which is responsible for the vasoconstrictor effect of pre-pro-endothelin-1 in addition to its possible direct vasoconstrictor effect on kidney vasculature. phosphoramidon 41-55 endothelin-1 Oryctolagus cuniculus 186-198 7896062-13 1994 From these results it was concluded that phosphoramidon-sensitive endothelin-converting enzyme, probably localized in microvasculature of the kidney, can convert pre-pro-endothelin-1 to endothelin-1 which is responsible for the vasoconstrictor effect of pre-pro-endothelin-1 in addition to its possible direct vasoconstrictor effect on kidney vasculature. phosphoramidon 41-55 endothelin-1 Oryctolagus cuniculus 186-198 7834180-0 1994 Synergistic inhibition by BQ-123 and BQ-788 of endothelin-1-induced contractions of the rabbit pulmonary artery. BQ 788 37-43 endothelin-1 Oryctolagus cuniculus 47-59 7834180-2 1994 However, the combination of BQ-123 and BQ-788 completely inhibited the ET-1-induced contraction. cyclo(Trp-Asp-Pro-Val-Leu) 28-34 endothelin-1 Oryctolagus cuniculus 71-75 7834180-2 1994 However, the combination of BQ-123 and BQ-788 completely inhibited the ET-1-induced contraction. BQ 788 39-45 endothelin-1 Oryctolagus cuniculus 71-75 7834180-4 1994 In receptor binding assays, [125I]-ET-1 specific binding to pulmonary arterial membranes was inhibited by BQ-123 (1 microM) by approximately 20% and additive treatment with BQ-788 (1 microM) completely inhibited the BQ-123-resistant component of [125I]-ET-1 specific binding. cyclo(Trp-Asp-Pro-Val-Leu) 106-112 endothelin-1 Oryctolagus cuniculus 35-39 7834180-4 1994 In receptor binding assays, [125I]-ET-1 specific binding to pulmonary arterial membranes was inhibited by BQ-123 (1 microM) by approximately 20% and additive treatment with BQ-788 (1 microM) completely inhibited the BQ-123-resistant component of [125I]-ET-1 specific binding. BQ 788 173-179 endothelin-1 Oryctolagus cuniculus 35-39 7834180-4 1994 In receptor binding assays, [125I]-ET-1 specific binding to pulmonary arterial membranes was inhibited by BQ-123 (1 microM) by approximately 20% and additive treatment with BQ-788 (1 microM) completely inhibited the BQ-123-resistant component of [125I]-ET-1 specific binding. BQ 788 173-179 endothelin-1 Oryctolagus cuniculus 253-257 7834180-4 1994 In receptor binding assays, [125I]-ET-1 specific binding to pulmonary arterial membranes was inhibited by BQ-123 (1 microM) by approximately 20% and additive treatment with BQ-788 (1 microM) completely inhibited the BQ-123-resistant component of [125I]-ET-1 specific binding. cyclo(Trp-Asp-Pro-Val-Leu) 216-222 endothelin-1 Oryctolagus cuniculus 35-39 7834180-5 1994 The present study demonstrates synergistic inhibition by BQ-123 and BQ-788 of ET-1-induced contraction of the rabbit pulmonary artery and the coexistence of ETA and ETB receptors, suggesting that the activation of either only ETA or only ETB receptors may be sufficient to cause complete vasoconstriction. cyclo(Trp-Asp-Pro-Val-Leu) 57-63 endothelin-1 Oryctolagus cuniculus 78-82 7834180-5 1994 The present study demonstrates synergistic inhibition by BQ-123 and BQ-788 of ET-1-induced contraction of the rabbit pulmonary artery and the coexistence of ETA and ETB receptors, suggesting that the activation of either only ETA or only ETB receptors may be sufficient to cause complete vasoconstriction. BQ 788 68-74 endothelin-1 Oryctolagus cuniculus 78-82 8051724-4 1994 Electrical field stimulation of CC preparations contracted by NA or endothelin-1 produced frequency-dependent and tetrodotoxin-sensitive relaxations. Tetrodotoxin 114-126 endothelin-1 Oryctolagus cuniculus 68-80 7846742-8 1994 The majority of metabolites had no effect on release of these analytes; however, GM1, at 5,000 micrograms/L resulted in a significant (p < 0.05) increase in ET-1, while GM9 at both 500 and 5,000 micrograms/L resulted in a significant (p < 0.01) decrease in PGI2 from mesangial cells. G(M1) Ganglioside 81-84 endothelin-1 Oryctolagus cuniculus 160-164 7964290-2 1994 Since, in previous studies in the rabbit, sex steroids greatly affected uterine endothelin-1 (ET-1) immunolocalization and binding, we sought to compare results obtained in a relatively steroid-deprived uterus (postmenopausal women) with those obtained in late pregnancy. Steroids 46-54 endothelin-1 Oryctolagus cuniculus 80-92 7964290-2 1994 Since, in previous studies in the rabbit, sex steroids greatly affected uterine endothelin-1 (ET-1) immunolocalization and binding, we sought to compare results obtained in a relatively steroid-deprived uterus (postmenopausal women) with those obtained in late pregnancy. Steroids 46-54 endothelin-1 Oryctolagus cuniculus 94-98 7964290-2 1994 Since, in previous studies in the rabbit, sex steroids greatly affected uterine endothelin-1 (ET-1) immunolocalization and binding, we sought to compare results obtained in a relatively steroid-deprived uterus (postmenopausal women) with those obtained in late pregnancy. Steroids 46-53 endothelin-1 Oryctolagus cuniculus 94-98 8093098-2 1994 The stimulating effect of ET-1 was inhibited by BQ-123, a specific antagonist of the ETA receptor. cyclo(Trp-Asp-Pro-Val-Leu) 48-54 endothelin-1 Oryctolagus cuniculus 26-30 8093098-5 1994 Non-selective calcium channel blockers such as econazole and La3+ strongly inhibited ET-1-activated migration but had little effect on fMLP-activated migration, underlining the importance of Ca2+ influx for ET-1-activated migration. Econazole 47-56 endothelin-1 Oryctolagus cuniculus 85-89 8093098-5 1994 Non-selective calcium channel blockers such as econazole and La3+ strongly inhibited ET-1-activated migration but had little effect on fMLP-activated migration, underlining the importance of Ca2+ influx for ET-1-activated migration. Econazole 47-56 endothelin-1 Oryctolagus cuniculus 207-211 8093098-5 1994 Non-selective calcium channel blockers such as econazole and La3+ strongly inhibited ET-1-activated migration but had little effect on fMLP-activated migration, underlining the importance of Ca2+ influx for ET-1-activated migration. lanthanum(3+) 61-65 endothelin-1 Oryctolagus cuniculus 85-89 8093098-5 1994 Non-selective calcium channel blockers such as econazole and La3+ strongly inhibited ET-1-activated migration but had little effect on fMLP-activated migration, underlining the importance of Ca2+ influx for ET-1-activated migration. lanthanum(3+) 61-65 endothelin-1 Oryctolagus cuniculus 207-211 8093098-7 1994 ET-1-activated migration of electroporated cells was completely blocked by low concentrations of calcium-channel blockers such as verapamil and nitrendipine. Verapamil 130-139 endothelin-1 Oryctolagus cuniculus 0-4 8093098-7 1994 ET-1-activated migration of electroporated cells was completely blocked by low concentrations of calcium-channel blockers such as verapamil and nitrendipine. Nitrendipine 144-156 endothelin-1 Oryctolagus cuniculus 0-4 8093098-9 1994 This suggests that calcium derived from intracellular stores is required for migration activated by ET-1. Calcium 19-26 endothelin-1 Oryctolagus cuniculus 100-104 8023985-2 1994 Because the concentration-response curve for ET-1 was biphasic (whereas that for ET-3 was monophasic), ET-1 had a PIE greater than ET-3 up to 10(-8) M. ET-1 induced a PIE at 3 x 10(-14) M and higher, which reached a plateau of 10-20% of Isomax at 10(-12) M (first phase); the curve became steeper at 10(-9) M and higher (second phase), achieving the maximal response at 10(-7) M to 3 x 10(-7) M. An ETA-selective antagonist, BQ-123, did not affect the PIE of ET-1 up to 10(-7) M; it abolished the first phase at 10(-6) M but did not affect the second phase. cyclo(Trp-Asp-Pro-Val-Leu) 425-431 endothelin-1 Oryctolagus cuniculus 103-107 7952871-6 1994 Postjunctionally, ET-1 enhanced the responses to exogenous adenosine 5"-triphosphate (ATP) and did not influence those to exogenous noradrenaline (NA). Adenosine Triphosphate 59-84 endothelin-1 Oryctolagus cuniculus 18-22 7952871-6 1994 Postjunctionally, ET-1 enhanced the responses to exogenous adenosine 5"-triphosphate (ATP) and did not influence those to exogenous noradrenaline (NA). Adenosine Triphosphate 86-89 endothelin-1 Oryctolagus cuniculus 18-22 7952871-8 1994 ET-1 increased the contractile responses to short-lasting and to long-lasting electrical field stimulation at a frequency of 5 or 10 Hz, showing a tendency towards decreasing the prazosin-sensitive component and increasing the mATP-sensitive component of the contractile responses. Prazosin 179-187 endothelin-1 Oryctolagus cuniculus 0-4 7952871-8 1994 ET-1 increased the contractile responses to short-lasting and to long-lasting electrical field stimulation at a frequency of 5 or 10 Hz, showing a tendency towards decreasing the prazosin-sensitive component and increasing the mATP-sensitive component of the contractile responses. matp 227-231 endothelin-1 Oryctolagus cuniculus 0-4 7952871-10 1994 In prazosin-treated preparations ET-1 increased the residual mATP-sensitive responses and this effect was more pronounced after yohimbine. Prazosin 3-11 endothelin-1 Oryctolagus cuniculus 33-37 7952871-10 1994 In prazosin-treated preparations ET-1 increased the residual mATP-sensitive responses and this effect was more pronounced after yohimbine. matp 61-65 endothelin-1 Oryctolagus cuniculus 33-37 7952871-10 1994 In prazosin-treated preparations ET-1 increased the residual mATP-sensitive responses and this effect was more pronounced after yohimbine. Yohimbine 128-137 endothelin-1 Oryctolagus cuniculus 33-37 7952871-12 1994 In mATP-treated preparations ET-1 increased the residual electrically-induced contractions and this increase was abolished after yohimbine. Yohimbine 129-138 endothelin-1 Oryctolagus cuniculus 29-33 7952871-14 1994 It is suggested that ET-1 modulates co-transmission in the rabbit saphenous artery by potentiating postjunctionally the purinergic component of the contractile responses to both exogenous ATP or electrical stimulation. Adenosine Triphosphate 188-191 endothelin-1 Oryctolagus cuniculus 21-25 8023985-2 1994 Because the concentration-response curve for ET-1 was biphasic (whereas that for ET-3 was monophasic), ET-1 had a PIE greater than ET-3 up to 10(-8) M. ET-1 induced a PIE at 3 x 10(-14) M and higher, which reached a plateau of 10-20% of Isomax at 10(-12) M (first phase); the curve became steeper at 10(-9) M and higher (second phase), achieving the maximal response at 10(-7) M to 3 x 10(-7) M. An ETA-selective antagonist, BQ-123, did not affect the PIE of ET-1 up to 10(-7) M; it abolished the first phase at 10(-6) M but did not affect the second phase. cyclo(Trp-Asp-Pro-Val-Leu) 425-431 endothelin-1 Oryctolagus cuniculus 103-107 8023985-2 1994 Because the concentration-response curve for ET-1 was biphasic (whereas that for ET-3 was monophasic), ET-1 had a PIE greater than ET-3 up to 10(-8) M. ET-1 induced a PIE at 3 x 10(-14) M and higher, which reached a plateau of 10-20% of Isomax at 10(-12) M (first phase); the curve became steeper at 10(-9) M and higher (second phase), achieving the maximal response at 10(-7) M to 3 x 10(-7) M. An ETA-selective antagonist, BQ-123, did not affect the PIE of ET-1 up to 10(-7) M; it abolished the first phase at 10(-6) M but did not affect the second phase. cyclo(Trp-Asp-Pro-Val-Leu) 425-431 endothelin-1 Oryctolagus cuniculus 103-107 8032665-7 1994 Infusion of FR 139317 (1 microM) significantly attenuated the increase in CPP caused by ET-1 (30 pmol: 3 +/- 1 mmHg, 100 pmol: 8 +/- 2 mmHg; n = 8). FR 139317 12-21 endothelin-1 Oryctolagus cuniculus 88-92 7932217-2 1994 The mechanism underlying the vasoconstriction induced by endothelin-1 (ET-1) was investigated by measuring the intracellular concentration of Ca2+ ([Ca2+]i), isometric force and phosphorylation of the myosin light chain (MLC) in endothelium-denuded unskinned and beta-escin-treated skinned smooth muscle from resistance vessels of the rabbit mesentery. Escin 263-273 endothelin-1 Oryctolagus cuniculus 57-69 7932217-2 1994 The mechanism underlying the vasoconstriction induced by endothelin-1 (ET-1) was investigated by measuring the intracellular concentration of Ca2+ ([Ca2+]i), isometric force and phosphorylation of the myosin light chain (MLC) in endothelium-denuded unskinned and beta-escin-treated skinned smooth muscle from resistance vessels of the rabbit mesentery. Escin 263-273 endothelin-1 Oryctolagus cuniculus 71-75 7932217-6 1994 Nicardipine completely blocked the ET-1-induced increase in [Ca2+]i. Nicardipine 0-11 endothelin-1 Oryctolagus cuniculus 35-39 7932217-7 1994 BQ-123 (an inhibitor of the ETA receptor) blocked the ET-1-induced contraction but IRL 1620 (Suc-[Glu9,Ala11,15]-ET-1(8-21), an agonist of the ETB receptor) failed to induce contraction. cyclo(Trp-Asp-Pro-Val-Leu) 0-6 endothelin-1 Oryctolagus cuniculus 54-58 7932217-9 1994 In ionomycin- and 70 mM K(+)-treated strips, ET-1 shifted the [Ca2+]i-force relationship to the left and enhanced the maximum amplitude of contraction induced by 2.6 mM Ca2+. Ionomycin 3-12 endothelin-1 Oryctolagus cuniculus 45-49 7932217-11 1994 ET-1 with GTP shifted both the Ca(2+)-force and Ca(2+)-MLC phosphorylation relationships to the left without significant changes in the maximum responses. Guanosine Triphosphate 10-13 endothelin-1 Oryctolagus cuniculus 0-4 7932217-12 1994 ET-1 with GTP did not change the relationship between force and MLC phosphorylation. Guanosine Triphosphate 10-13 endothelin-1 Oryctolagus cuniculus 0-4 7932217-14 1994 These results indicate that the sensitivity of MLC phosphorylation to Ca2+ is enhanced both by ET-1 with GTP and by PDBu. Guanosine Triphosphate 105-108 endothelin-1 Oryctolagus cuniculus 95-99 7932217-17 1994 However, PKC19-36 only partly inhibited the enhancement produced by ET-1 with GTP on the Ca(2+)-induced responses. Guanosine Triphosphate 78-81 endothelin-1 Oryctolagus cuniculus 68-72 7932217-19 1994 By contrast, BQ-123, neomycin and guanosine 5"-O-(2-thiodiphosphate) (GDP beta S) each abolished the ET-1-induced enhancement of the contraction induced by 0.3 microM Ca2+. cyclo(Trp-Asp-Pro-Val-Leu) 13-19 endothelin-1 Oryctolagus cuniculus 101-105 7932217-19 1994 By contrast, BQ-123, neomycin and guanosine 5"-O-(2-thiodiphosphate) (GDP beta S) each abolished the ET-1-induced enhancement of the contraction induced by 0.3 microM Ca2+. Neomycin 21-29 endothelin-1 Oryctolagus cuniculus 101-105 7932217-19 1994 By contrast, BQ-123, neomycin and guanosine 5"-O-(2-thiodiphosphate) (GDP beta S) each abolished the ET-1-induced enhancement of the contraction induced by 0.3 microM Ca2+. guanosine 5'-O-(2-thiodiphosphate) 34-68 endothelin-1 Oryctolagus cuniculus 101-105 7932217-19 1994 By contrast, BQ-123, neomycin and guanosine 5"-O-(2-thiodiphosphate) (GDP beta S) each abolished the ET-1-induced enhancement of the contraction induced by 0.3 microM Ca2+. guanosine 5'-O-(2-thiodiphosphate) 70-80 endothelin-1 Oryctolagus cuniculus 101-105 7521681-0 1994 Alteration by Iloprost of the vasospastic effects of endothelin-1 in rabbit cerebral vessels. Iloprost 14-22 endothelin-1 Oryctolagus cuniculus 53-65 7521681-1 1994 The reversal of endothelin-1 induced cerebral vasospasm with Iloprost was studied in the rabbit. Iloprost 61-69 endothelin-1 Oryctolagus cuniculus 16-28 7521681-3 1994 A potent antagonistic effect of Iloprost against ET-1 was observed in each of the parameters measured. Iloprost 32-40 endothelin-1 Oryctolagus cuniculus 49-53 8184985-3 1994 ET-1 (icv) caused significant increases in mean arterial pressure (MAP), renal sympathetic nerve activity (RSNA), plasma catecholamines, and vasopressin levels. rsna 107-111 endothelin-1 Oryctolagus cuniculus 0-4 7923900-2 1994 Intravenous endothelin-1 releases endothelium-derived relaxing factor (EDRF) and prostacyclin from the lungs, and substantial pulmonary clearance is claimed. Epoprostenol 81-93 endothelin-1 Oryctolagus cuniculus 12-24 8184985-3 1994 ET-1 (icv) caused significant increases in mean arterial pressure (MAP), renal sympathetic nerve activity (RSNA), plasma catecholamines, and vasopressin levels. Catecholamines 121-135 endothelin-1 Oryctolagus cuniculus 0-4 8184985-4 1994 Naloxone pretreatment significantly augmented the increases in MAP and RSNA induced by icv ET-1. Naloxone 0-8 endothelin-1 Oryctolagus cuniculus 91-95 8184985-8 1994 These results suggest that intravenous naloxone acts in the central nervous system to attenuate the baroreflex sensitivity, which might augment the increases in MAP and RSNA induced by icv ET-1 in conscious rabbits. Naloxone 39-47 endothelin-1 Oryctolagus cuniculus 189-193 8184985-8 1994 These results suggest that intravenous naloxone acts in the central nervous system to attenuate the baroreflex sensitivity, which might augment the increases in MAP and RSNA induced by icv ET-1 in conscious rabbits. rsna 169-173 endothelin-1 Oryctolagus cuniculus 189-193 8194356-1 1994 The characterization and localization of binding sites for endothelin-1 (ET-1) labeled with iodine 125I were investigated in homogenized tissues and sections of Harder"s glands of normal rabbits. Iodine 92-98 endothelin-1 Oryctolagus cuniculus 59-71 8174163-5 1994 Arginine vasopressin [2(0.6) nM] and endothelin-1 [1.5(1) nM] induced similar reductions of coronary resting flow but the pressure induced flow increases were significantly greater than in the presence of L-NNA. Nitroarginine 205-210 endothelin-1 Oryctolagus cuniculus 37-49 8138949-5 1994 Thus, in rabbit sphincter: 1) ET-1 and ET-2, two potent ETA receptor agonists, induced IP3 production and contraction at a much higher rate than ET-3, a weak ETA agonist. Inositol 1,4,5-Trisphosphate 87-90 endothelin-1 Oryctolagus cuniculus 30-34 8194356-1 1994 The characterization and localization of binding sites for endothelin-1 (ET-1) labeled with iodine 125I were investigated in homogenized tissues and sections of Harder"s glands of normal rabbits. Iodine 92-98 endothelin-1 Oryctolagus cuniculus 73-77 8020873-7 1994 Thus, differences in the pattern of desensitisation of both pressor responses and phosphatidylinositol metabolism were observed for noradrenaline and endothelin-1 suggesting that the nature of the 2nd messenger involved in signal transduction is not the only determinant of agonist desensitisation. Phosphatidylinositols 82-102 endothelin-1 Oryctolagus cuniculus 150-162 8300340-4 1994 Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium. Fura-2 206-212 endothelin-1 Oryctolagus cuniculus 125-129 8020873-0 1994 Agonist desensitisation of alpha 1 adrenoceptors and endothelin-1 receptors coupled to phosphatidylinositol metabolism. Phosphatidylinositols 87-107 endothelin-1 Oryctolagus cuniculus 53-65 8300340-4 1994 Endothelin receptor linkage to cell signaling pathways was determined based on measurements of the dose dependent effects of ET-1, ET-2, and ET-3 on intracellular Ca2+ concentration ([Ca2+]i) transients in fura-2-loaded cells, and of ET-1 on phosphoinositide turnover and cAMP accumulation in the isolated rabbit corneal epithelium. Fura-2 206-212 endothelin-1 Oryctolagus cuniculus 234-238 8300340-6 1994 ET-1 (1 nM) maximally stimulated [3H]-thymidine uptake by twofold (EC50 = 0.3 nM). 3h]-thymidine 34-47 endothelin-1 Oryctolagus cuniculus 0-4 8300340-9 1994 Intracellular mobilization was linked to increases in IP3 turnover because 1 microM ET-1 increased IP3 content by 48% from its control value (EC50 = 23 nM), whereas Ca2+ influx occurred through a non-L-type Ca2+ channel because preexposure to 1 microM nicardipine did not affect either the height or the duration of a [Ca2+]i transient. Inositol 1,4,5-Trisphosphate 54-57 endothelin-1 Oryctolagus cuniculus 84-88 8300340-9 1994 Intracellular mobilization was linked to increases in IP3 turnover because 1 microM ET-1 increased IP3 content by 48% from its control value (EC50 = 23 nM), whereas Ca2+ influx occurred through a non-L-type Ca2+ channel because preexposure to 1 microM nicardipine did not affect either the height or the duration of a [Ca2+]i transient. Inositol 1,4,5-Trisphosphate 99-102 endothelin-1 Oryctolagus cuniculus 84-88 8300340-9 1994 Intracellular mobilization was linked to increases in IP3 turnover because 1 microM ET-1 increased IP3 content by 48% from its control value (EC50 = 23 nM), whereas Ca2+ influx occurred through a non-L-type Ca2+ channel because preexposure to 1 microM nicardipine did not affect either the height or the duration of a [Ca2+]i transient. Nicardipine 252-263 endothelin-1 Oryctolagus cuniculus 84-88 8300340-10 1994 One micromolar of ET-1 was required to elicit a significant increase in cAMP accumulation of 69% from its control value. Cyclic AMP 72-76 endothelin-1 Oryctolagus cuniculus 18-22 8057752-1 1994 We investigated the effect of maturation on the superoxide anion production of rabbit alveolar macrophages by endothelin-1, N-formyl-methionyl-leucyl-phenylalanine, and phorbol 12-myristate 13-acetate. Superoxides 48-64 endothelin-1 Oryctolagus cuniculus 110-122 7906490-0 1994 [Evaluation of bunazosin hydrochloride with a model of ocular circulation disturbance induced by endothelin-1]. bunazosin 15-38 endothelin-1 Oryctolagus cuniculus 97-109 8246919-3 1993 ET-1 inhibited 125I-ET-1 binding to RSV in a monophasic manner, with an inhibition constant (Ki) of 0.08 +/- 0.02 nM and a slope factor of 0.9 +/- 0.1. Respiratory Syncytial Virus Vaccines 36-39 endothelin-1 Oryctolagus cuniculus 0-4 7906490-4 1994 Administration of bunazosin hydrochloride had no significant effect on the capillary blood flow in the ONH in normal eyes, but it inhibited the decrease of blood flow for at least 5 hours in the endothelin-1 treated eyes. bunazosin 18-41 endothelin-1 Oryctolagus cuniculus 195-207 7906490-5 1994 The relative caliber of the retinal artery decreased in the endothelin-1 treated eyes (maximum at 5 hours), but previous administration of bunazosin hydrochloride inhibited the decrease. bunazosin 139-162 endothelin-1 Oryctolagus cuniculus 60-72 7906490-6 1994 Thus, Bunazosin hydrochloride inhibits the disturbance of ONH circulation induced by endothelin-1 and promises to be useful also with regard to the ocular circulation. bunazosin 6-29 endothelin-1 Oryctolagus cuniculus 85-97 8258832-3 1993 These compounds were shown to have high antagonist activities in ET-1-induced vasoconstriction of porcine coronary artery (pA2 7.4-7.7) and in Sarafotoxin S6c-induced vasoconstriction of rabbit pulmonary artery ([Thr18, gamma-MeLeu19]ET-1: pA2 8.4). sarafotoxin 143-154 endothelin-1 Oryctolagus cuniculus 65-69 8258832-3 1993 These compounds were shown to have high antagonist activities in ET-1-induced vasoconstriction of porcine coronary artery (pA2 7.4-7.7) and in Sarafotoxin S6c-induced vasoconstriction of rabbit pulmonary artery ([Thr18, gamma-MeLeu19]ET-1: pA2 8.4). sarafotoxin 143-154 endothelin-1 Oryctolagus cuniculus 234-238 8258832-3 1993 These compounds were shown to have high antagonist activities in ET-1-induced vasoconstriction of porcine coronary artery (pA2 7.4-7.7) and in Sarafotoxin S6c-induced vasoconstriction of rabbit pulmonary artery ([Thr18, gamma-MeLeu19]ET-1: pA2 8.4). s6c 155-158 endothelin-1 Oryctolagus cuniculus 65-69 8258832-3 1993 These compounds were shown to have high antagonist activities in ET-1-induced vasoconstriction of porcine coronary artery (pA2 7.4-7.7) and in Sarafotoxin S6c-induced vasoconstriction of rabbit pulmonary artery ([Thr18, gamma-MeLeu19]ET-1: pA2 8.4). UNII-PYZ33YLR8A 213-218 endothelin-1 Oryctolagus cuniculus 65-69 8258832-3 1993 These compounds were shown to have high antagonist activities in ET-1-induced vasoconstriction of porcine coronary artery (pA2 7.4-7.7) and in Sarafotoxin S6c-induced vasoconstriction of rabbit pulmonary artery ([Thr18, gamma-MeLeu19]ET-1: pA2 8.4). gamma-meleu19 220-233 endothelin-1 Oryctolagus cuniculus 65-69 8258832-4 1993 Among these compounds, [Thr18, gamma-MeLeu19]ET-1 has the desirable characteristic of possessing no agonist activity at either receptor subtype. UNII-PYZ33YLR8A 24-29 endothelin-1 Oryctolagus cuniculus 45-49 8287895-0 1993 Endothelin-1 enhances vasoconstrictor responses to exogenously administered and neurogenically released ATP in rabbit isolated perfused arteries. Adenosine Triphosphate 104-107 endothelin-1 Oryctolagus cuniculus 0-12 8287895-1 1993 Vasoconstrictor responses to ATP were enhanced in a concentration-dependent-manner by endothelin-1 (0.1-1 nM) in isolated perfused segments of rabbit ear artery. Adenosine Triphosphate 29-32 endothelin-1 Oryctolagus cuniculus 86-98 8287895-2 1993 Higher concentrations of endothelin-1 (3-10 nM) also enhanced vasoconstrictor responses to ATP. Adenosine Triphosphate 91-94 endothelin-1 Oryctolagus cuniculus 25-37 8287895-3 1993 The enhancement of ATP responses by endothelin-1 (0.1-10 nM) was not affected by nicardipine (10 nM). Adenosine Triphosphate 19-22 endothelin-1 Oryctolagus cuniculus 36-48 8287895-4 1993 The ATP-mediated component of the vasoconstrictor response to perivascular nerve stimulation in the rabbit jejunal artery was enhanced by endothelin-1 to a similar extent as that to exogenously administered ATP. Adenosine Triphosphate 4-7 endothelin-1 Oryctolagus cuniculus 138-150 8246919-3 1993 ET-1 inhibited 125I-ET-1 binding to RSV in a monophasic manner, with an inhibition constant (Ki) of 0.08 +/- 0.02 nM and a slope factor of 0.9 +/- 0.1. Respiratory Syncytial Virus Vaccines 36-39 endothelin-1 Oryctolagus cuniculus 20-24 8246919-6 1993 The ET-A-selective peptide BQ-123 inhibited 125I-ET-1 binding in a biphasic manner, with Ki values of 10.4 +/- 1.9 nM and 3.2 +/- 0.9 microM. et-a 4-8 endothelin-1 Oryctolagus cuniculus 49-53 8246919-6 1993 The ET-A-selective peptide BQ-123 inhibited 125I-ET-1 binding in a biphasic manner, with Ki values of 10.4 +/- 1.9 nM and 3.2 +/- 0.9 microM. cyclo(Trp-Asp-Pro-Val-Leu) 27-33 endothelin-1 Oryctolagus cuniculus 49-53 8246919-10 1993 ET-1 and BQ-123 inhibited 125I-ET-3 binding in RSV with Ki values of 40 +/- 7 pM and 7.2 microM, respectively, whereas inhibition curves for ET-3 and the ET-B receptor-selective agonist sarafotoxin S6c (S6c) were best fit to two-site models. Respiratory Syncytial Virus Vaccines 47-50 endothelin-1 Oryctolagus cuniculus 0-4 8246919-10 1993 ET-1 and BQ-123 inhibited 125I-ET-3 binding in RSV with Ki values of 40 +/- 7 pM and 7.2 microM, respectively, whereas inhibition curves for ET-3 and the ET-B receptor-selective agonist sarafotoxin S6c (S6c) were best fit to two-site models. sarafotoxin 186-197 endothelin-1 Oryctolagus cuniculus 0-4 8143240-4 1993 However, in rings precontracted to a similar tone by endothelin-1, the relaxation elicited by carbachol was reduced in the vein but remained unchanged in the artery. Carbachol 94-103 endothelin-1 Oryctolagus cuniculus 53-65 8262745-11 1993 The action of endothelin-1 was inhibited to a greater extent by iohexol and mannitol than by ioxaglate and diatrizoate. Iohexol 64-71 endothelin-1 Oryctolagus cuniculus 14-26 8262745-11 1993 The action of endothelin-1 was inhibited to a greater extent by iohexol and mannitol than by ioxaglate and diatrizoate. Mannitol 76-84 endothelin-1 Oryctolagus cuniculus 14-26 8262745-11 1993 The action of endothelin-1 was inhibited to a greater extent by iohexol and mannitol than by ioxaglate and diatrizoate. Ioxaglic Acid 93-102 endothelin-1 Oryctolagus cuniculus 14-26 8262745-11 1993 The action of endothelin-1 was inhibited to a greater extent by iohexol and mannitol than by ioxaglate and diatrizoate. Diatrizoate 107-118 endothelin-1 Oryctolagus cuniculus 14-26 8143240-7 1993 The ETA receptor antagonist BQ123 shifted, to the right, the concentration-response curves of endothelin-1 on endothelium-denuded saphenous artery (pA2 = 7.25). cyclo(Trp-Asp-Pro-Val-Leu) 28-33 endothelin-1 Oryctolagus cuniculus 94-106 8143240-9 1993 In vein but not in artery, endothelin-1 and sarafotoxin S6c induced an endothelium-dependent relaxation, which was increased, in the case of endothelin-1, in the presence of BQ123.2+. cyclo(Trp-Asp-Pro-Val-Leu) 174-179 endothelin-1 Oryctolagus cuniculus 27-39 8143240-9 1993 In vein but not in artery, endothelin-1 and sarafotoxin S6c induced an endothelium-dependent relaxation, which was increased, in the case of endothelin-1, in the presence of BQ123.2+. cyclo(Trp-Asp-Pro-Val-Leu) 174-179 endothelin-1 Oryctolagus cuniculus 141-153 8225777-10 1993 Similar to authentic ET-1, the product of chymosin treatment caused contraction of isolated rabbit aortic rings, and pre-incubation of chymosin with pepstatin A abolished this contractile response. pepstatin 149-160 endothelin-1 Oryctolagus cuniculus 21-25 7686534-0 1993 Potentiation of norepinephrine-induced contractions by endothelin-1 in the rabbit aorta. Norepinephrine 16-30 endothelin-1 Oryctolagus cuniculus 55-67 7692176-0 1993 Nitric oxide and prostacyclin influence coronary vasomotor tone in perfused rabbit heart and modulate endothelin-1 activity. Nitric Oxide 0-12 endothelin-1 Oryctolagus cuniculus 102-114 7692176-0 1993 Nitric oxide and prostacyclin influence coronary vasomotor tone in perfused rabbit heart and modulate endothelin-1 activity. Epoprostenol 17-29 endothelin-1 Oryctolagus cuniculus 102-114 7692176-3 1993 Furthermore, the increase in resting CPP induced by graded doses of endothelin-1 (ET-1 0.6-160 pmol), was further augmented by blocking of prostaglandin biosynthesis with indomethacin (3 microM) and was substantially reduced when the rate of formation of PGI2 was enhanced by defibrotide (200 micrograms/ml). Endothelin-1 82-86 endothelin-1 Oryctolagus cuniculus 68-80 7692176-3 1993 Furthermore, the increase in resting CPP induced by graded doses of endothelin-1 (ET-1 0.6-160 pmol), was further augmented by blocking of prostaglandin biosynthesis with indomethacin (3 microM) and was substantially reduced when the rate of formation of PGI2 was enhanced by defibrotide (200 micrograms/ml). Prostaglandins 139-152 endothelin-1 Oryctolagus cuniculus 68-80 7692176-3 1993 Furthermore, the increase in resting CPP induced by graded doses of endothelin-1 (ET-1 0.6-160 pmol), was further augmented by blocking of prostaglandin biosynthesis with indomethacin (3 microM) and was substantially reduced when the rate of formation of PGI2 was enhanced by defibrotide (200 micrograms/ml). Indomethacin 171-183 endothelin-1 Oryctolagus cuniculus 68-80 7692176-3 1993 Furthermore, the increase in resting CPP induced by graded doses of endothelin-1 (ET-1 0.6-160 pmol), was further augmented by blocking of prostaglandin biosynthesis with indomethacin (3 microM) and was substantially reduced when the rate of formation of PGI2 was enhanced by defibrotide (200 micrograms/ml). Epoprostenol 255-259 endothelin-1 Oryctolagus cuniculus 68-80 7692176-3 1993 Furthermore, the increase in resting CPP induced by graded doses of endothelin-1 (ET-1 0.6-160 pmol), was further augmented by blocking of prostaglandin biosynthesis with indomethacin (3 microM) and was substantially reduced when the rate of formation of PGI2 was enhanced by defibrotide (200 micrograms/ml). defibrotide 276-287 endothelin-1 Oryctolagus cuniculus 68-80 7686534-1 1993 Subthreshold concentrations of endothelin-1 potentiated the norepinephrine-induced contraction in isometrically mounted rings of the rabbit aorta. Norepinephrine 60-74 endothelin-1 Oryctolagus cuniculus 31-43 7686534-2 1993 Pretreatment with endothelin-1 (0.1 nM) for 10 minutes increased the sensitivity of the aortic rings to norepinephrine without affecting the maximal contraction. Norepinephrine 104-118 endothelin-1 Oryctolagus cuniculus 18-30 7686534-7 1993 We conclude that endothelin-1 potentiation of the norepinephrine-induced contraction occurs in the absence of changes in stimulated Ca2+ entry and is endothelium independent. Norepinephrine 50-64 endothelin-1 Oryctolagus cuniculus 17-29 8365455-0 1993 Endothelin-1 induces vasoconstriction and prostacyclin release via the activation of endothelin ETA receptors in the perfused rabbit kidney. Epoprostenol 42-54 endothelin-1 Oryctolagus cuniculus 0-12 8365455-3 1993 Similarly, the release of prostacyclin triggered by intra-arterial infusion of endothelin-1 (10 nM) was significantly reduced in a concentration-dependent manner when the kidney was pretreated with BQ-123 (0.5-1 microM). Epoprostenol 26-38 endothelin-1 Oryctolagus cuniculus 79-91 8365455-3 1993 Similarly, the release of prostacyclin triggered by intra-arterial infusion of endothelin-1 (10 nM) was significantly reduced in a concentration-dependent manner when the kidney was pretreated with BQ-123 (0.5-1 microM). cyclo(Trp-Asp-Pro-Val-Leu) 198-204 endothelin-1 Oryctolagus cuniculus 79-91 8365455-6 1993 These results confirm our previous observations suggesting that pressor responses and prostanoid release induced by endothelin-1 are mediated via the selective activation of ETA receptors in the perfused rabbit kidney. Prostaglandins 86-96 endothelin-1 Oryctolagus cuniculus 116-128 8358553-6 1993 Intravenous infusion of FR139317 at 0.2 (n = 4) or 0.6 mg kg-1 min-1 (n = 4) inhibited the ET-1 pressor response by 83 or 89%, respectively. FR 139317 24-32 endothelin-1 Oryctolagus cuniculus 91-95 8358553-7 1993 Infusion of PD145065 at 0.2 (n = 4) or 0.6 mg kg-1 min-1 (n = 4) inhibited the ET-1-induced increase in MAP by 79 or 75%, respectively. PD 145065 12-20 endothelin-1 Oryctolagus cuniculus 79-83 8358553-9 1993 The transient depressor response (-16 +/- 3 mmHg) which preceded the rise in blood pressure induced by ET-1 (1 nmol kg-1, i.a., n = 8) was enhanced by an intravenous infusion of FR139317 (0.6 mg kg-1 min-1) to -35 +/- 5 mmHg (P < 0.05, n = 4). FR 139317 178-186 endothelin-1 Oryctolagus cuniculus 103-107 8358553-20 1993 In contrast, intravenous infusion of PD145065 (0.6 mg kg-1 min-1) abolished the anti-aggregatory effects of ET-1.5. PD 145065 37-45 endothelin-1 Oryctolagus cuniculus 108-112 8358553-22 1993 In contrast, PD145065 antagonizes the pressor and depressor responses to ET-1 and abolishes the anti-aggregatory effects of the peptide.6. PD 145065 13-21 endothelin-1 Oryctolagus cuniculus 73-77 8328335-6 1993 Fluorescein angiograms revealed that retinal blood flow was interrupted transiently by injection of a high dose (10(-10) mole) of ET-1. Fluorescein 0-11 endothelin-1 Oryctolagus cuniculus 130-134 7682141-2 1993 Endothelin-1 infusion (5-40 pmol kg-1 min-1) in the normal anaesthetized rabbit, produced a dose-dependent increase in mean arterial blood pressure (MAP) and reduced renal blood flow (RBF) and glomerular filtration rate (GFR), when compared with an equivalent infusion of physiological saline. Sodium Chloride 286-292 endothelin-1 Oryctolagus cuniculus 0-12 7509954-1 1993 BQ-123, a selective endothelin A (ETA) receptor antagonist, was used to study the receptors involved in the ET-1-induced release of prostacyclin (PGI2) in perfused rat lung and rabbit kidney and the pressor effects of ET-1 in rabbit renal vasculature. cyclo(Trp-Asp-Pro-Val-Leu) 0-6 endothelin-1 Oryctolagus cuniculus 218-222 8143240-2 1993 Although artery rings were more sensitive than those from vein to the contractile action of phenylephrine, endothelin-1 was about three times more potent as a contractile agonist on vein than on artery. Phenylephrine 92-105 endothelin-1 Oryctolagus cuniculus 107-119 8387043-0 1993 Endothelin-1-induced phosphoinositide hydrolysis and contraction in isolated rabbit detrusor and urethral smooth muscle. Phosphatidylinositols 21-37 endothelin-1 Oryctolagus cuniculus 0-12 8387043-2 1993 Endothelin-1 (ET-1) caused a concentration-dependent increase in the formation of inositol phosphates (IPs) in isolated rabbit detrusor and urethral smooth muscle preparations prelabelled with myo-[3H]inositol. Inositol Phosphates 82-101 endothelin-1 Oryctolagus cuniculus 0-12 8387043-2 1993 Endothelin-1 (ET-1) caused a concentration-dependent increase in the formation of inositol phosphates (IPs) in isolated rabbit detrusor and urethral smooth muscle preparations prelabelled with myo-[3H]inositol. Inositol Phosphates 82-101 endothelin-1 Oryctolagus cuniculus 14-18 8387043-2 1993 Endothelin-1 (ET-1) caused a concentration-dependent increase in the formation of inositol phosphates (IPs) in isolated rabbit detrusor and urethral smooth muscle preparations prelabelled with myo-[3H]inositol. Inositol Phosphates 103-106 endothelin-1 Oryctolagus cuniculus 0-12 8387043-2 1993 Endothelin-1 (ET-1) caused a concentration-dependent increase in the formation of inositol phosphates (IPs) in isolated rabbit detrusor and urethral smooth muscle preparations prelabelled with myo-[3H]inositol. Inositol Phosphates 103-106 endothelin-1 Oryctolagus cuniculus 14-18 8387043-2 1993 Endothelin-1 (ET-1) caused a concentration-dependent increase in the formation of inositol phosphates (IPs) in isolated rabbit detrusor and urethral smooth muscle preparations prelabelled with myo-[3H]inositol. myo-[3h]inositol 193-209 endothelin-1 Oryctolagus cuniculus 0-12 8387043-2 1993 Endothelin-1 (ET-1) caused a concentration-dependent increase in the formation of inositol phosphates (IPs) in isolated rabbit detrusor and urethral smooth muscle preparations prelabelled with myo-[3H]inositol. myo-[3h]inositol 193-209 endothelin-1 Oryctolagus cuniculus 14-18 8387043-5 1993 The increase in IPs accumulation found after exposure of detrusor tissue to ET-1 (10(-7) M) for 2 hr (250 +/- 38%, n = 7) was not significantly different from that found in the urethra (279 +/- 40%, n = 6), when expressed as per cent of corresponding control values. Inositol Phosphates 16-19 endothelin-1 Oryctolagus cuniculus 76-80 8387043-13 1993 ET-1-induced contractions in urethral preparations were markedly inhibited by Ni2+ (3 x 10(-4) M), whereas the effect of Ni2+ in the detrusor was less pronounced. Nickel(2+) 78-82 endothelin-1 Oryctolagus cuniculus 0-4 8387043-15 1993 The results suggest that ET-1 stimulates phosphoinositide hydrolysis in the rabbit detrusor and urethra. Phosphatidylinositols 41-57 endothelin-1 Oryctolagus cuniculus 25-29 8387043-16 1993 Both IPs formation and contractile activation evoked by ET-1 are dependent on extracellular Ca2+. Inositol Phosphates 5-8 endothelin-1 Oryctolagus cuniculus 56-60 7509926-2 1993 Endothelin-1 (ET-1), sarafotoxin S6c, and the linear endothelin peptide Ala11,15-ET-1[8-21] evoked approximately monophasic concentration-dependent increases in force development in the rabbit jugular vein (rank order of potency: sarafotoxin S6c > ET-1 > Ala11,15-ET-1[8-21]). sarafotoxin 230-241 endothelin-1 Oryctolagus cuniculus 0-12 7509926-2 1993 Endothelin-1 (ET-1), sarafotoxin S6c, and the linear endothelin peptide Ala11,15-ET-1[8-21] evoked approximately monophasic concentration-dependent increases in force development in the rabbit jugular vein (rank order of potency: sarafotoxin S6c > ET-1 > Ala11,15-ET-1[8-21]). sarafotoxin 230-241 endothelin-1 Oryctolagus cuniculus 81-85 7509926-2 1993 Endothelin-1 (ET-1), sarafotoxin S6c, and the linear endothelin peptide Ala11,15-ET-1[8-21] evoked approximately monophasic concentration-dependent increases in force development in the rabbit jugular vein (rank order of potency: sarafotoxin S6c > ET-1 > Ala11,15-ET-1[8-21]). sarafotoxin 230-241 endothelin-1 Oryctolagus cuniculus 81-85 7509926-2 1993 Endothelin-1 (ET-1), sarafotoxin S6c, and the linear endothelin peptide Ala11,15-ET-1[8-21] evoked approximately monophasic concentration-dependent increases in force development in the rabbit jugular vein (rank order of potency: sarafotoxin S6c > ET-1 > Ala11,15-ET-1[8-21]). sarafotoxin 230-241 endothelin-1 Oryctolagus cuniculus 81-85 7509926-5 1993 Force evoked by ET-1 was minimally affected by the relatively ETA-selective (in comparison with ETB) receptor antagonists BQ-123 and FR139317. FR 139317 133-141 endothelin-1 Oryctolagus cuniculus 16-20 7509981-9 1993 These results suggest that ET-1 decreases oxygen supply to the cardiac muscles by constricting coronary vessels and that this, in turn, worsens the ischemic condition of the heart to extend the infarct size. Oxygen 42-48 endothelin-1 Oryctolagus cuniculus 27-31 7509954-3 1993 In rabbit kidney, the PGI2 release induced by ET-1 (10 nM) was abolished by a 15-min pretreatment with BQ-123 (1 microM). Epoprostenol 22-26 endothelin-1 Oryctolagus cuniculus 46-50 7510001-3 1993 When injected OT, the contractile responses of the RbJV to ET-1 were > or = 100-200 times more than those to bET-1. rbjv 51-55 endothelin-1 Oryctolagus cuniculus 59-63 7510001-4 1993 However, at least 10 times the ET-1 dose given OT was needed TK to produce an equivalent contraction of the RbJV, showing that ET-1 was being inactivated or removed by the kidney. rbjv 108-112 endothelin-1 Oryctolagus cuniculus 31-35 7509954-3 1993 In rabbit kidney, the PGI2 release induced by ET-1 (10 nM) was abolished by a 15-min pretreatment with BQ-123 (1 microM). cyclo(Trp-Asp-Pro-Val-Leu) 103-109 endothelin-1 Oryctolagus cuniculus 46-50 7510001-7 1993 Phosphoramidon (10 microM) infused TK blocked (92 +/- 1% inhibition) the renal responses to bET-1 and reduced the overflow of ET-1-like material onto the tissues without affecting ET-1-induced renal vasoconstriction. phosphoramidon 0-14 endothelin-1 Oryctolagus cuniculus 93-97 7509954-4 1993 In both preparations the ET-1-induced release of PGI2 was fully restored 60 min after the interruption of BQ-123 infusion. Epoprostenol 49-53 endothelin-1 Oryctolagus cuniculus 25-29 7510001-7 1993 Phosphoramidon (10 microM) infused TK blocked (92 +/- 1% inhibition) the renal responses to bET-1 and reduced the overflow of ET-1-like material onto the tissues without affecting ET-1-induced renal vasoconstriction. phosphoramidon 0-14 endothelin-1 Oryctolagus cuniculus 126-130 7510001-8 1993 Incubation of bET-1 with rabbit renal cortical microsomes (100 micrograms protein) resulted in the generation of ET-1-like activity, as assessed by bioassay, which was inhibited by phosphoramidon (10 microM). phosphoramidon 181-195 endothelin-1 Oryctolagus cuniculus 15-19 7509954-4 1993 In both preparations the ET-1-induced release of PGI2 was fully restored 60 min after the interruption of BQ-123 infusion. cyclo(Trp-Asp-Pro-Val-Leu) 106-112 endothelin-1 Oryctolagus cuniculus 25-29 7509954-6 1993 The pressor responses to ET-1 were abolished by BQ-123 (0.1 microM), and 60 min after interruption of the infusion of the antagonist, the responses to ET-1 were restored to 68% and 99% of control values, respectively. cyclo(Trp-Asp-Pro-Val-Leu) 48-54 endothelin-1 Oryctolagus cuniculus 25-29 1451739-0 1992 The vasoconstrictor action of big endothelin-1 is phosphoramidon-sensitive in rabbit saphenous artery, but not in saphenous vein. phosphoramidon 50-64 endothelin-1 Oryctolagus cuniculus 34-46 8341129-3 1993 Fifteen minutes after BQ-123 (1 mumole) was injected into the posterior vitreous body, the dose-response curve of ET-1 was significantly shifted to the right. cyclo(Trp-Asp-Pro-Val-Leu) 22-28 endothelin-1 Oryctolagus cuniculus 114-118 1451739-4 1992 Phosphoramidon (100 microM), a metalloproteinase inhibitor, antagonised the contractile action of big endothelin-1 in the artery but not in the vein. phosphoramidon 0-14 endothelin-1 Oryctolagus cuniculus 102-114 1379005-1 1992 We assessed the acute effect of 17 beta-estradiol on coronary artery constrictor responses to endothelin-1. Estradiol 32-49 endothelin-1 Oryctolagus cuniculus 94-106 1489666-1 1992 The stimulation of phospholipase D (PLD) activity by endothelin-1 (ET1) was investigated in rabbit iris sphincter prelabelled with [3H]myristic acid. [3h]myristic acid 131-148 endothelin-1 Oryctolagus cuniculus 53-65 1489666-1 1992 The stimulation of phospholipase D (PLD) activity by endothelin-1 (ET1) was investigated in rabbit iris sphincter prelabelled with [3H]myristic acid. [3h]myristic acid 131-148 endothelin-1 Oryctolagus cuniculus 67-70 1489666-2 1992 In the presence of 0.5% ethanol, ET1 caused a time- and dose-dependent increase in the production of [3H]phosphatidylethanol ([3H]PEt). Ethanol 24-31 endothelin-1 Oryctolagus cuniculus 33-36 1489666-2 1992 In the presence of 0.5% ethanol, ET1 caused a time- and dose-dependent increase in the production of [3H]phosphatidylethanol ([3H]PEt). 3h]phosphatidylethanol 102-124 endothelin-1 Oryctolagus cuniculus 33-36 1489666-2 1992 In the presence of 0.5% ethanol, ET1 caused a time- and dose-dependent increase in the production of [3H]phosphatidylethanol ([3H]PEt). Tritium 102-104 endothelin-1 Oryctolagus cuniculus 33-36 1489666-5 1992 This value is appreciably lower than the EC50 we previously obtained for ET1-induced inositol trisphosphate production (45 nM), but considerably higher than that for arachidonic acid release (1 nM). inositol 1,2,3-trisphosphate 85-107 endothelin-1 Oryctolagus cuniculus 73-76 1329561-0 1992 Effects of CD-349 and 8-BrcGMP on isoproterenol-induced relaxation in rabbit aorta precontracted with endothelin-1. Isoproterenol 34-47 endothelin-1 Oryctolagus cuniculus 102-114 1329561-2 1992 The Iso (10(-8)-10(-5) M)-induced relaxation responses in rabbit aorta precontracted with 1-2 x 10(-7) M ET-1 were augmented by pretreatment with CD-349 (10(-9)-10(-5) M) in a concentration-dependent manner. Cadmium 146-149 endothelin-1 Oryctolagus cuniculus 105-109 1329561-3 1992 The effects of CD-349 on Iso-induced relaxation of the aortic strips precontracted with ET-1 were inhibited by treatment with methylene blue (10(-5) M) and oxyhemoglobin (10(-5) M), whereas they were augmented by treatment with N omega-nitro-L-arginine (10(-4) M). CD 349 15-21 endothelin-1 Oryctolagus cuniculus 88-92 1329561-3 1992 The effects of CD-349 on Iso-induced relaxation of the aortic strips precontracted with ET-1 were inhibited by treatment with methylene blue (10(-5) M) and oxyhemoglobin (10(-5) M), whereas they were augmented by treatment with N omega-nitro-L-arginine (10(-4) M). Methylene Blue 126-140 endothelin-1 Oryctolagus cuniculus 88-92 1329561-3 1992 The effects of CD-349 on Iso-induced relaxation of the aortic strips precontracted with ET-1 were inhibited by treatment with methylene blue (10(-5) M) and oxyhemoglobin (10(-5) M), whereas they were augmented by treatment with N omega-nitro-L-arginine (10(-4) M). Nitroarginine 228-252 endothelin-1 Oryctolagus cuniculus 88-92 1325299-3 1992 Addition of 100 nM ET-1 plus 10 microM GTP significantly enhanced myofilament Ca2+ sensitivity as compared with the addition of Ca2+ alone (EC50, 0.47 microM Ca2+ for Ca2+ alone and 0.13 microM Ca2+ for ET-1 plus (GTP). Guanosine Triphosphate 39-42 endothelin-1 Oryctolagus cuniculus 203-207 1325299-3 1992 Addition of 100 nM ET-1 plus 10 microM GTP significantly enhanced myofilament Ca2+ sensitivity as compared with the addition of Ca2+ alone (EC50, 0.47 microM Ca2+ for Ca2+ alone and 0.13 microM Ca2+ for ET-1 plus (GTP). Guanosine Triphosphate 214-217 endothelin-1 Oryctolagus cuniculus 19-23 1325299-5 1992 ET-1-induced contractions were relaxed at a constant [Ca2+] by the addition of 30 microM cAMP or cGMP, demonstrating a direct effect of the cyclic nucleotides on contractile regulation. Cyclic AMP 89-93 endothelin-1 Oryctolagus cuniculus 0-4 1325299-5 1992 ET-1-induced contractions were relaxed at a constant [Ca2+] by the addition of 30 microM cAMP or cGMP, demonstrating a direct effect of the cyclic nucleotides on contractile regulation. Cyclic GMP 97-101 endothelin-1 Oryctolagus cuniculus 0-4 1325299-5 1992 ET-1-induced contractions were relaxed at a constant [Ca2+] by the addition of 30 microM cAMP or cGMP, demonstrating a direct effect of the cyclic nucleotides on contractile regulation. Nucleotides, Cyclic 140-158 endothelin-1 Oryctolagus cuniculus 0-4 1325299-6 1992 Inhibition of protein kinase C activity by 100 nM staurosporine relaxed ET-1 plus GTP-induced contractions, and pretreatment with 40 microM chelerythrine inhibited the ET-1 plus GTP increase in force. Staurosporine 50-63 endothelin-1 Oryctolagus cuniculus 72-76 1325299-6 1992 Inhibition of protein kinase C activity by 100 nM staurosporine relaxed ET-1 plus GTP-induced contractions, and pretreatment with 40 microM chelerythrine inhibited the ET-1 plus GTP increase in force. chelerythrine 140-153 endothelin-1 Oryctolagus cuniculus 168-172 1398887-3 1992 Endothelin-1 and -2 (10 nM), endothelin-3 (100 nM), and big endothelin-1 and -2 (100 nM) are potent enhancers of prostacyclin release without inducing any release of thromboxane B2 in the perfused kidney. Epoprostenol 113-125 endothelin-1 Oryctolagus cuniculus 0-19 1398887-3 1992 Endothelin-1 and -2 (10 nM), endothelin-3 (100 nM), and big endothelin-1 and -2 (100 nM) are potent enhancers of prostacyclin release without inducing any release of thromboxane B2 in the perfused kidney. Epoprostenol 113-125 endothelin-1 Oryctolagus cuniculus 60-79 1398887-3 1992 Endothelin-1 and -2 (10 nM), endothelin-3 (100 nM), and big endothelin-1 and -2 (100 nM) are potent enhancers of prostacyclin release without inducing any release of thromboxane B2 in the perfused kidney. Thromboxane B2 166-180 endothelin-1 Oryctolagus cuniculus 60-79 1398887-5 1992 A metalloprotease inhibitor, phosphoramidon (100 microM, 60 minutes), reduced the prostanoid release and pressor responses induced by big endothelin-1 and -2 without affecting the response induced by endothelin-1, -2, and -3. phosphoramidon 29-43 endothelin-1 Oryctolagus cuniculus 138-157 1398887-5 1992 A metalloprotease inhibitor, phosphoramidon (100 microM, 60 minutes), reduced the prostanoid release and pressor responses induced by big endothelin-1 and -2 without affecting the response induced by endothelin-1, -2, and -3. Prostaglandins 82-92 endothelin-1 Oryctolagus cuniculus 138-157 1398887-6 1992 These results suggest the presence of a phosphoramidon-sensitive endothelin converting enzyme that converts the precursors of endothelin-1 and -2, but not of endothelin-3, in the renal vasculature of the rabbit. phosphoramidon 40-54 endothelin-1 Oryctolagus cuniculus 126-145 1415557-3 1992 ET-1 elicited significant dose-dependent (10(-12)-10(-6) M) increases in ASM cell number, with a mean potency (i.e., -log mean effective dose) of action of 9.82-log M. ET-1 also acutely stimulated intracellular inositol 1,4,5-trisphosphate accumulation. Inositol 1,4,5-Trisphosphate 211-239 endothelin-1 Oryctolagus cuniculus 0-4 1326626-0 1992 Concentration- and time-dependence of phosphoinositide hydrolysis induced by endothelin-1 in relation to the positive inotropic effect in the rabbit ventricular myocardium. Phosphatidylinositols 38-54 endothelin-1 Oryctolagus cuniculus 77-89 1326626-1 1992 The effects of endothelin-1 on phosphoinositide hydrolysis in relation to the positive inotropic effect of the compound were studied in the rabbit ventricular myocardium. Phosphatidylinositols 31-47 endothelin-1 Oryctolagus cuniculus 15-27 1326626-2 1992 Endothelin-1 elicited a concentration- and time-dependent accumulation of [3H]inositol monophosphate (IP1) in ventricular muscle slices prelabeled with myo-[3H]inositol in concentrations similar to those that caused a positive inotropic effect. [3h]inositol monophosphate 74-100 endothelin-1 Oryctolagus cuniculus 0-12 1326626-2 1992 Endothelin-1 elicited a concentration- and time-dependent accumulation of [3H]inositol monophosphate (IP1) in ventricular muscle slices prelabeled with myo-[3H]inositol in concentrations similar to those that caused a positive inotropic effect. Isopenicillin N 102-105 endothelin-1 Oryctolagus cuniculus 0-12 1326626-2 1992 Endothelin-1 elicited a concentration- and time-dependent accumulation of [3H]inositol monophosphate (IP1) in ventricular muscle slices prelabeled with myo-[3H]inositol in concentrations similar to those that caused a positive inotropic effect. myo-[3h]inositol 152-168 endothelin-1 Oryctolagus cuniculus 0-12 1326626-3 1992 The EC50 value of endothelin-1, both for the induction of [3H]IP1 accumulation and for the positive inotropic effect was 6 x 10(-9) M. The endothelin-induced positive inotropic effect was linearly related to [3H]IP1 accumulation. Tritium 59-61 endothelin-1 Oryctolagus cuniculus 18-30 1326626-3 1992 The EC50 value of endothelin-1, both for the induction of [3H]IP1 accumulation and for the positive inotropic effect was 6 x 10(-9) M. The endothelin-induced positive inotropic effect was linearly related to [3H]IP1 accumulation. Isopenicillin N 62-65 endothelin-1 Oryctolagus cuniculus 18-30 1326626-3 1992 The EC50 value of endothelin-1, both for the induction of [3H]IP1 accumulation and for the positive inotropic effect was 6 x 10(-9) M. The endothelin-induced positive inotropic effect was linearly related to [3H]IP1 accumulation. Tritium 209-211 endothelin-1 Oryctolagus cuniculus 18-30 1326626-3 1992 The EC50 value of endothelin-1, both for the induction of [3H]IP1 accumulation and for the positive inotropic effect was 6 x 10(-9) M. The endothelin-induced positive inotropic effect was linearly related to [3H]IP1 accumulation. Isopenicillin N 212-215 endothelin-1 Oryctolagus cuniculus 18-30 1326626-5 1992 After administration of endothelin-1, [3H]IP3 accumulated rapidly and transiently before the development of the positive inotropic effect. [3h]ip3 38-45 endothelin-1 Oryctolagus cuniculus 24-36 1326626-8 1992 Phorbol 12,13-dibutyrate inhibited both the [3H] IP1 accumulation and positive inotropic effect induced by endothelin-1. Phorbol 12,13-Dibutyrate 0-24 endothelin-1 Oryctolagus cuniculus 107-119 1326626-9 1992 The present results indicate that an acceleration of phosphoinositide hydrolysis induced by activation of endothelin-1 receptors may be responsible for induction of the positive inotropic effect of endothelin-1 on rabbit ventricular myocardium. Phosphatidylinositols 53-69 endothelin-1 Oryctolagus cuniculus 106-118 1326626-9 1992 The present results indicate that an acceleration of phosphoinositide hydrolysis induced by activation of endothelin-1 receptors may be responsible for induction of the positive inotropic effect of endothelin-1 on rabbit ventricular myocardium. Phosphatidylinositols 53-69 endothelin-1 Oryctolagus cuniculus 198-210 1392057-6 1992 injection of increasing doses of endothelin-1 (ET) (0.01-0.3 microgram) caused a weak but long-lasting and dose-dependent vasoconstriction of the thoracic aorta; its effect was less potent than that of angiotensin II or norepinephrine when their peak responses were compared. Norepinephrine 220-234 endothelin-1 Oryctolagus cuniculus 33-45 1392057-6 1992 injection of increasing doses of endothelin-1 (ET) (0.01-0.3 microgram) caused a weak but long-lasting and dose-dependent vasoconstriction of the thoracic aorta; its effect was less potent than that of angiotensin II or norepinephrine when their peak responses were compared. Norepinephrine 220-234 endothelin-1 Oryctolagus cuniculus 47-49 1379005-0 1992 Acute effect of 17 beta-estradiol on rabbit coronary artery contractile responses to endothelin-1. Estradiol 16-33 endothelin-1 Oryctolagus cuniculus 85-97 1379005-2 1992 17 beta-Estradiol significantly shifted endothelin-1, calcium, or BAY K 8644 concentration-dependent contraction curves to the right in endothelium-denuded coronary arteries isolated from nonpregnant female rabbits. Estradiol 3-17 endothelin-1 Oryctolagus cuniculus 40-52 1379005-5 1992 The -log ED50 of endothelin-1 was 9.2 +/- 0.08 in control and 8.8 +/- 0.1, 8.4 +/- 0.07, and 8.1 +/- 0.12 after incubation with 17 beta-estradiol (3, 10, and 30 microM, respectively). Estradiol 131-145 endothelin-1 Oryctolagus cuniculus 17-29 1379005-8 1992 17 beta-Estradiol and verapamil induced dose-dependent relaxation in both endothelium-intact or -denuded coronary arteries submaximally precontracted by endothelin-1. Estradiol 3-17 endothelin-1 Oryctolagus cuniculus 153-165 1379005-8 1992 17 beta-Estradiol and verapamil induced dose-dependent relaxation in both endothelium-intact or -denuded coronary arteries submaximally precontracted by endothelin-1. Verapamil 22-31 endothelin-1 Oryctolagus cuniculus 153-165 1379005-10 1992 These data suggest that 17 beta-estradiol attenuates the rabbit coronary artery contraction induced by endothelin-1 via an endothelium-independent mechanism, possibly by affecting calcium influx. Estradiol 24-41 endothelin-1 Oryctolagus cuniculus 103-115 1379005-10 1992 These data suggest that 17 beta-estradiol attenuates the rabbit coronary artery contraction induced by endothelin-1 via an endothelium-independent mechanism, possibly by affecting calcium influx. Calcium 180-187 endothelin-1 Oryctolagus cuniculus 103-115 1504743-8 1992 L-NOARG (30 microM) also augmented contractions of endothelium-intact tissues to noradrenaline, prostaglandin F2 alpha, and to a lesser degree endothelin-1. Nitroarginine 0-7 endothelin-1 Oryctolagus cuniculus 143-155 1321928-2 1992 Whether ET-1 exerts a direct stimulating effect on sodium reabsorption in the renal proximal convoluted tubule, the dominant locus of sodium reabsorption in the nephron, is currently unknown. Sodium 51-57 endothelin-1 Oryctolagus cuniculus 8-12 1320879-2 1992 BQ-123 competitively antagonized endothelin-1-induced contractions in rabbit aorta, increases in inositol phosphates in cultured rat vascular smooth muscle A10 cells, and binding of [125I]endothelin-1 to the cloned ETA receptor cDNA expressed in Cos 7 cells. cyclo(Trp-Asp-Pro-Val-Leu) 0-6 endothelin-1 Oryctolagus cuniculus 33-45 1321928-8 1992 The data implicate ET-1 as a novel, direct and specific modulator of sodium reabsorption in the proximal tubule. Sodium 69-75 endothelin-1 Oryctolagus cuniculus 19-23 1593712-2 1992 Both endothelin-1 and noradrenaline caused a time- and concentration-dependent increase in the accumulation of 3H-inositol phosphates in preparations prelabelled with 3H-myo-inositol. 3h-inositol phosphates 111-133 endothelin-1 Oryctolagus cuniculus 5-17 1629095-7 1992 Phosphoramidon, an inhibitor of metalloproteinase, suppressed the pressor effect of big ET-1 (P less than 0.01) and the increase in the concentration of ET-1 in the perfusate (P less than 0.05). phosphoramidon 0-14 endothelin-1 Oryctolagus cuniculus 88-92 1629095-7 1992 Phosphoramidon, an inhibitor of metalloproteinase, suppressed the pressor effect of big ET-1 (P less than 0.01) and the increase in the concentration of ET-1 in the perfusate (P less than 0.05). phosphoramidon 0-14 endothelin-1 Oryctolagus cuniculus 153-157 1593712-2 1992 Both endothelin-1 and noradrenaline caused a time- and concentration-dependent increase in the accumulation of 3H-inositol phosphates in preparations prelabelled with 3H-myo-inositol. 3h-myo-inositol 167-182 endothelin-1 Oryctolagus cuniculus 5-17 1593712-4 1992 At 60 minutes, the mean increases in 3H-inositol inositol phosphates induced by 3 x 10(-7) M endothelin-1 and 10(-3) M noradrenaline amounted to 341 and 530% of time-matched controls, respectively. 3h-inositol inositol phosphates 37-68 endothelin-1 Oryctolagus cuniculus 93-112 1593712-4 1992 At 60 minutes, the mean increases in 3H-inositol inositol phosphates induced by 3 x 10(-7) M endothelin-1 and 10(-3) M noradrenaline amounted to 341 and 530% of time-matched controls, respectively. Norepinephrine 119-132 endothelin-1 Oryctolagus cuniculus 93-112 1326745-0 1992 Endothelin-1 stimulates chloride and potassium secretion in rabbit descending colon. Chlorides 24-32 endothelin-1 Oryctolagus cuniculus 0-12 1326745-0 1992 Endothelin-1 stimulates chloride and potassium secretion in rabbit descending colon. Potassium 37-46 endothelin-1 Oryctolagus cuniculus 0-12 1326745-4 1992 The secretory effect of ET-1 is associated with increased release of prostaglandin E2 from the serosal surface of the mucosa. Dinoprostone 69-85 endothelin-1 Oryctolagus cuniculus 24-28 1326745-5 1992 ET-1-induced Cl- secretion is completely inhibited by the loop diuretic bumetanide and by indomethacin and quinacrine, inhibitors of prostaglandin synthesis. Bumetanide 72-82 endothelin-1 Oryctolagus cuniculus 0-4 1326745-5 1992 ET-1-induced Cl- secretion is completely inhibited by the loop diuretic bumetanide and by indomethacin and quinacrine, inhibitors of prostaglandin synthesis. Indomethacin 90-102 endothelin-1 Oryctolagus cuniculus 0-4 1326745-5 1992 ET-1-induced Cl- secretion is completely inhibited by the loop diuretic bumetanide and by indomethacin and quinacrine, inhibitors of prostaglandin synthesis. Quinacrine 107-117 endothelin-1 Oryctolagus cuniculus 0-4 1326745-5 1992 ET-1-induced Cl- secretion is completely inhibited by the loop diuretic bumetanide and by indomethacin and quinacrine, inhibitors of prostaglandin synthesis. Prostaglandins 133-146 endothelin-1 Oryctolagus cuniculus 0-4 1326745-8 1992 It is concluded that ET-1 causes Cl- and K+ secretion by stimulating phospholipase A2 and release of prostaglandins, whereas Na+ transport is not altered. Prostaglandins 101-115 endothelin-1 Oryctolagus cuniculus 21-25 1590388-4 1992 In isolated, vascularly perfused rabbit stomach, various concentrations (10-400 mM) of ethanol infused into the celiac artery increased the perfusion pressure and released endothelin-1 (ET-1) from the gastric vasculature in a concentration-dependent manner. Ethanol 87-94 endothelin-1 Oryctolagus cuniculus 172-184 1590388-4 1992 In isolated, vascularly perfused rabbit stomach, various concentrations (10-400 mM) of ethanol infused into the celiac artery increased the perfusion pressure and released endothelin-1 (ET-1) from the gastric vasculature in a concentration-dependent manner. Ethanol 87-94 endothelin-1 Oryctolagus cuniculus 186-190 1345777-8 1992 Increasing shear stress by a strong vasoconstriction (1 nM endothelin-1) at constant flow was also accompanied by a 2.5-fold increase in platelet cGMP content. Cyclic GMP 146-150 endothelin-1 Oryctolagus cuniculus 59-71 1314581-5 1992 The selective ETA receptor antagonist BQ-123 blunted the ET-1 contractions in rabbit carotid artery, but not in saphenous vein. cyclo(Trp-Asp-Pro-Val-Leu) 38-44 endothelin-1 Oryctolagus cuniculus 57-61 1404663-7 1992 Rabbit bladder strips in organ chambers contracted when exposed to endothelin-1 and this response was partially attenuated by calcium channel blockers or by removal of extracellular calcium. Calcium 126-133 endothelin-1 Oryctolagus cuniculus 67-79 1404663-10 1992 Endothelin-1 caused a small but consistent attenuation of the atropine sensitive component of the neurogenic contraction, while it had no effect on the atropine resistant component. Atropine 62-70 endothelin-1 Oryctolagus cuniculus 0-12 1657445-9 1991 In endothelium-intact strips of the porcine coronary artery, ET-1 significantly increased the concentration of 6-ketoprostaglandin F1 alpha but did not modify the cellular concentration of either cAMP or cGMP. 6-ketoprostaglandin 111-130 endothelin-1 Oryctolagus cuniculus 61-65 1305913-0 1992 Contribution of GTP-binding protein to the actions of endothelin-1 on rabbit parasympathetic neurons. Guanosine Triphosphate 16-19 endothelin-1 Oryctolagus cuniculus 54-66 1687465-3 1991 These basal values were decreased by azelastine with an IC50 value of 1.1 +/- 0.3 x 10(-4) M. Endothelin-1 (10(-7) M) induced a rapid increase in free cytosolic calcium up to 806 +/- 314 nM, which returned to normal levels in 3-5 min. azelastine 37-47 endothelin-1 Oryctolagus cuniculus 94-106 1687465-3 1991 These basal values were decreased by azelastine with an IC50 value of 1.1 +/- 0.3 x 10(-4) M. Endothelin-1 (10(-7) M) induced a rapid increase in free cytosolic calcium up to 806 +/- 314 nM, which returned to normal levels in 3-5 min. Calcium 161-168 endothelin-1 Oryctolagus cuniculus 94-106 1797325-10 1991 L-NAME (10(-4) M) increased the maximal contraction at 37 degrees C, and both the sensitivity and maximal contraction at 24 degrees C of intact arteries to endothelin-1. NG-Nitroarginine Methyl Ester 0-6 endothelin-1 Oryctolagus cuniculus 156-168 1797325-15 1991 These results suggest that cooling decreases sensitivity of cutaneous arteries (ear artery) to endothelin-1 probably by increasing the availability of endothelial nitric oxide. Nitric Oxide 163-175 endothelin-1 Oryctolagus cuniculus 95-107 1884967-0 1991 Effect of ethanol on endothelin-1 release from gastric vasculature. Ethanol 10-17 endothelin-1 Oryctolagus cuniculus 21-33 1657858-3 1991 Reduction of calcium availability with verapamil, cadmium, or a calcium-free buffer significantly blunts the increase in pressure caused by ET-1. Calcium 13-20 endothelin-1 Oryctolagus cuniculus 140-144 1657858-3 1991 Reduction of calcium availability with verapamil, cadmium, or a calcium-free buffer significantly blunts the increase in pressure caused by ET-1. Verapamil 39-48 endothelin-1 Oryctolagus cuniculus 140-144 1657858-3 1991 Reduction of calcium availability with verapamil, cadmium, or a calcium-free buffer significantly blunts the increase in pressure caused by ET-1. Cadmium 50-57 endothelin-1 Oryctolagus cuniculus 140-144 1657858-3 1991 Reduction of calcium availability with verapamil, cadmium, or a calcium-free buffer significantly blunts the increase in pressure caused by ET-1. Calcium 64-71 endothelin-1 Oryctolagus cuniculus 140-144 1657858-5 1991 Three different inhibitors of protein kinase C, phloretin, staurosporine, and dihydrosphingosine, significantly diminish the response to ET-1. Phloretin 48-57 endothelin-1 Oryctolagus cuniculus 137-141 1657858-5 1991 Three different inhibitors of protein kinase C, phloretin, staurosporine, and dihydrosphingosine, significantly diminish the response to ET-1. Staurosporine 59-72 endothelin-1 Oryctolagus cuniculus 137-141 1657858-5 1991 Three different inhibitors of protein kinase C, phloretin, staurosporine, and dihydrosphingosine, significantly diminish the response to ET-1. safingol 78-96 endothelin-1 Oryctolagus cuniculus 137-141 1657858-6 1991 Indomethacin and a thromboxane synthase inhibitor partially decrease the response to the highest concentration of ET-1. Indomethacin 0-12 endothelin-1 Oryctolagus cuniculus 114-118 1657858-7 1991 Isoproterenol and dibutyryl adenosine 3",5"-cyclic monophosphate (cAMP) are significantly more effective in preventing the vasoconstriction caused by ET-1 than are nitroprusside and guanosine 5"-cyclic monophosphate (cGMP) analogues. Isoproterenol 0-13 endothelin-1 Oryctolagus cuniculus 150-154 1657858-7 1991 Isoproterenol and dibutyryl adenosine 3",5"-cyclic monophosphate (cAMP) are significantly more effective in preventing the vasoconstriction caused by ET-1 than are nitroprusside and guanosine 5"-cyclic monophosphate (cGMP) analogues. Bucladesine 18-64 endothelin-1 Oryctolagus cuniculus 150-154 1657858-7 1991 Isoproterenol and dibutyryl adenosine 3",5"-cyclic monophosphate (cAMP) are significantly more effective in preventing the vasoconstriction caused by ET-1 than are nitroprusside and guanosine 5"-cyclic monophosphate (cGMP) analogues. Cyclic AMP 66-70 endothelin-1 Oryctolagus cuniculus 150-154 1657858-7 1991 Isoproterenol and dibutyryl adenosine 3",5"-cyclic monophosphate (cAMP) are significantly more effective in preventing the vasoconstriction caused by ET-1 than are nitroprusside and guanosine 5"-cyclic monophosphate (cGMP) analogues. Cyclic GMP 217-221 endothelin-1 Oryctolagus cuniculus 150-154 1657858-9 1991 ET-1 appears to cause pulmonary vasoconstriction by increasing calcium entry and by activating protein kinase C. Vasodilators that increase cAMP are substantially more effective in preventing the increase in pressure than are drugs that increase cGMP. Calcium 63-70 endothelin-1 Oryctolagus cuniculus 0-4 1657858-9 1991 ET-1 appears to cause pulmonary vasoconstriction by increasing calcium entry and by activating protein kinase C. Vasodilators that increase cAMP are substantially more effective in preventing the increase in pressure than are drugs that increase cGMP. Cyclic AMP 140-144 endothelin-1 Oryctolagus cuniculus 0-4 1657858-9 1991 ET-1 appears to cause pulmonary vasoconstriction by increasing calcium entry and by activating protein kinase C. Vasodilators that increase cAMP are substantially more effective in preventing the increase in pressure than are drugs that increase cGMP. Cyclic GMP 246-250 endothelin-1 Oryctolagus cuniculus 0-4 1649147-2 1991 They found that ET1 is a potent agonist for IP3 production, DAG formation, and contraction in rabbit, dog, cat, and pig iris sphincters, and for cAMP formation in all species that were investigated--rabbit, dog, cat, pig, bovine, monkey, and human sphincters. Inositol 1,4,5-Trisphosphate 44-47 endothelin-1 Oryctolagus cuniculus 16-19 1649147-2 1991 They found that ET1 is a potent agonist for IP3 production, DAG formation, and contraction in rabbit, dog, cat, and pig iris sphincters, and for cAMP formation in all species that were investigated--rabbit, dog, cat, pig, bovine, monkey, and human sphincters. dag 60-63 endothelin-1 Oryctolagus cuniculus 16-19 1649147-6 1991 ET1-stimulated IP3 production is dose dependent with an EC50 of 45 nM, this value is about 100- and 56-fold lower than those we reported for substance P and carbachol, respectively. Carbachol 157-166 endothelin-1 Oryctolagus cuniculus 0-3 1649147-7 1991 ET1 also increased 32P labeling of PA more than 6-fold; and in rabbit sphincter, ET1 is a more potent agonist in contracting the sphincter than in contracting the dilator (the EC50 values for sphincter and dilator were 46 and 120 nM, respectively). Phosphorus-32 19-22 endothelin-1 Oryctolagus cuniculus 0-3 1884967-4 1991 Perfusion pressure and endothelin-1 concentration in effluent increased in a dose-dependent manner with increasing ethanol concentrations. Ethanol 115-122 endothelin-1 Oryctolagus cuniculus 23-35 1649147-7 1991 ET1 also increased 32P labeling of PA more than 6-fold; and in rabbit sphincter, ET1 is a more potent agonist in contracting the sphincter than in contracting the dilator (the EC50 values for sphincter and dilator were 46 and 120 nM, respectively). Phosphatidic Acids 35-37 endothelin-1 Oryctolagus cuniculus 0-3 1649147-2 1991 They found that ET1 is a potent agonist for IP3 production, DAG formation, and contraction in rabbit, dog, cat, and pig iris sphincters, and for cAMP formation in all species that were investigated--rabbit, dog, cat, pig, bovine, monkey, and human sphincters. Cyclic AMP 145-149 endothelin-1 Oryctolagus cuniculus 16-19 1649147-5 1991 In rabbit sphincter, ET1 induced a significant increase in IP3 production by 30 sec and reached a 6-fold level more than control within 1 and 5 min. Inositol 1,4,5-Trisphosphate 59-62 endothelin-1 Oryctolagus cuniculus 21-24 1649147-6 1991 ET1-stimulated IP3 production is dose dependent with an EC50 of 45 nM, this value is about 100- and 56-fold lower than those we reported for substance P and carbachol, respectively. Inositol 1,4,5-Trisphosphate 15-18 endothelin-1 Oryctolagus cuniculus 0-3 1845558-5 1991 Ethanol infusion caused a dose-dependent increase in gastric vascular resistance in perfused rabbit stomach, which was accompanied by an increased production of endothelin-1. Ethanol 0-7 endothelin-1 Oryctolagus cuniculus 161-173 2058688-3 1991 In TSM half-maximally contracted with acetylcholine (ACh), however, ET-1 elicited dual and opposing dose-dependent effects. Acetylcholine 38-51 endothelin-1 Oryctolagus cuniculus 68-72 2058688-3 1991 In TSM half-maximally contracted with acetylcholine (ACh), however, ET-1 elicited dual and opposing dose-dependent effects. Acetylcholine 53-56 endothelin-1 Oryctolagus cuniculus 68-72 2058688-5 1991 The relaxant responses were associated with significantly enhanced (P less than 0.001) ET-1-induced release of prostaglandins E2 and I2 in the adult tissues. Dinoprostone 111-128 endothelin-1 Oryctolagus cuniculus 87-91 2058688-6 1991 At higher doses (greater than 10(-9) M), ET-1 induced TSM contractions that were 1) attenuated to a relatively greater extent by the Ca2+ channel blocker, nifedipine (10(-5) M) in the 2-wk-old tissues and 2) associated with significantly (P less than 0.001) enhanced ET-1-stimulated accumulation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] in the immature TSM. Nifedipine 155-165 endothelin-1 Oryctolagus cuniculus 41-45 2058688-6 1991 At higher doses (greater than 10(-9) M), ET-1 induced TSM contractions that were 1) attenuated to a relatively greater extent by the Ca2+ channel blocker, nifedipine (10(-5) M) in the 2-wk-old tissues and 2) associated with significantly (P less than 0.001) enhanced ET-1-stimulated accumulation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] in the immature TSM. Nifedipine 155-165 endothelin-1 Oryctolagus cuniculus 267-271 2058688-6 1991 At higher doses (greater than 10(-9) M), ET-1 induced TSM contractions that were 1) attenuated to a relatively greater extent by the Ca2+ channel blocker, nifedipine (10(-5) M) in the 2-wk-old tissues and 2) associated with significantly (P less than 0.001) enhanced ET-1-stimulated accumulation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] in the immature TSM. Inositol 1,4,5-Trisphosphate 299-327 endothelin-1 Oryctolagus cuniculus 41-45 2058688-6 1991 At higher doses (greater than 10(-9) M), ET-1 induced TSM contractions that were 1) attenuated to a relatively greater extent by the Ca2+ channel blocker, nifedipine (10(-5) M) in the 2-wk-old tissues and 2) associated with significantly (P less than 0.001) enhanced ET-1-stimulated accumulation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] in the immature TSM. Inositol 1,4,5-Trisphosphate 329-341 endothelin-1 Oryctolagus cuniculus 41-45 2058688-8 1991 Collectively, these findings demonstrate that ET-1 exerts a potent duality of action in rabbit TSM which varies significantly with maturation, wherein 1) age-dependent differences in airway relaxation are associated with changes in the evoked release of bronchodilatory prostaglandins and 2) maturational differences in airway contraction are associated with changes in Ins(1,4,5)P3 accumulation and extracellular Ca2+ mobilization, coupled to differences in PKC activation. Prostaglandins 270-284 endothelin-1 Oryctolagus cuniculus 46-50 2060603-6 1991 The effects of ET-1, ET-2 and ET-3 on the IOP and blood-aqueous barrier, as well as the effects of ET-1 on regional blood flow in the eye, were abolished by pretreatment with indomethacin. Indomethacin 175-187 endothelin-1 Oryctolagus cuniculus 15-19 2060603-6 1991 The effects of ET-1, ET-2 and ET-3 on the IOP and blood-aqueous barrier, as well as the effects of ET-1 on regional blood flow in the eye, were abolished by pretreatment with indomethacin. Indomethacin 175-187 endothelin-1 Oryctolagus cuniculus 99-103 2060603-7 1991 Injection of 4 pmol ET-1 into the anterior chamber caused an increase in the concentration of prostaglandin E2 (PGE2) in the aqueous humor, most probably as a result of increased production of PGE2 in the anterior uvea. Dinoprostone 94-110 endothelin-1 Oryctolagus cuniculus 20-24 2060603-7 1991 Injection of 4 pmol ET-1 into the anterior chamber caused an increase in the concentration of prostaglandin E2 (PGE2) in the aqueous humor, most probably as a result of increased production of PGE2 in the anterior uvea. Dinoprostone 112-116 endothelin-1 Oryctolagus cuniculus 20-24 2060603-7 1991 Injection of 4 pmol ET-1 into the anterior chamber caused an increase in the concentration of prostaglandin E2 (PGE2) in the aqueous humor, most probably as a result of increased production of PGE2 in the anterior uvea. Dinoprostone 193-197 endothelin-1 Oryctolagus cuniculus 20-24 2060603-8 1991 The results indicate that the effects of ET-1, ET-2 and ET-3 in the rabbit eye are to a large extent mediated by arachidonic acid metabolites. Arachidonic Acid 113-129 endothelin-1 Oryctolagus cuniculus 41-45 1904341-0 1991 Endothelin-1 stimulates the release of arachidonic acid and prostaglandins in rabbit iris sphincter smooth muscle: activation of phospholipase A2. Arachidonic Acid 39-55 endothelin-1 Oryctolagus cuniculus 0-12 1904341-0 1991 Endothelin-1 stimulates the release of arachidonic acid and prostaglandins in rabbit iris sphincter smooth muscle: activation of phospholipase A2. Prostaglandins 60-74 endothelin-1 Oryctolagus cuniculus 0-12 1904341-4 1991 The AA released by ET1 appears to derive mainly from the phosphoinositides through phospholipase A2, rather than phospholipase C activation. Phosphatidylinositols 57-74 endothelin-1 Oryctolagus cuniculus 19-22 1904341-6 1991 (1) Pretreatment of the labeled sphincter with the phorbol ester, PDBu (100 nM) inhibited ET1-stimulated IP3 formation, but it potentiated ET1-stimulated AA release. Phorbol Esters 51-64 endothelin-1 Oryctolagus cuniculus 90-93 1904341-6 1991 (1) Pretreatment of the labeled sphincter with the phorbol ester, PDBu (100 nM) inhibited ET1-stimulated IP3 formation, but it potentiated ET1-stimulated AA release. Inositol 1,4,5-Trisphosphate 105-108 endothelin-1 Oryctolagus cuniculus 90-93 1904341-7 1991 (2) Pretreatment of the labeled tissue with isoproterenol (5 M) inhibited ET1-stimulated IP3 production without altering AA release. Isoproterenol 44-57 endothelin-1 Oryctolagus cuniculus 74-77 1904341-7 1991 (2) Pretreatment of the labeled tissue with isoproterenol (5 M) inhibited ET1-stimulated IP3 production without altering AA release. Inositol 1,4,5-Trisphosphate 89-92 endothelin-1 Oryctolagus cuniculus 74-77 1904341-9 1991 (4) There were considerable increases, rather than decreases, in 1, 2-diacyl-glycerol formation (1.2-folds) and its phosphorylated product, phosphatidic acid (2.6-folds) by ET1. 1,2-diacylglycerol 65-85 endothelin-1 Oryctolagus cuniculus 173-176 1904341-9 1991 (4) There were considerable increases, rather than decreases, in 1, 2-diacyl-glycerol formation (1.2-folds) and its phosphorylated product, phosphatidic acid (2.6-folds) by ET1. Phosphatidic Acids 140-157 endothelin-1 Oryctolagus cuniculus 173-176 1904341-10 1991 It is concluded that in the rabbit iris sphincter ET1 is a potent agonist for AA release and eicosanoid synthesis and that AA is released from phosphoinositides mainly through activation of phospholipase A2. Eicosanoids 93-103 endothelin-1 Oryctolagus cuniculus 50-53 1847589-2 1991 The staining was dramatically affected by subacute treatment with ovarian steroids: epithelial cells were predominantly positive in immature rabbits, whereas, in sex steroid-primed rabbits, ET-1 was mainly localized in the stromal compartment. Steroids 74-81 endothelin-1 Oryctolagus cuniculus 190-194 1847589-9 1991 Conversely, divalent ions like calcium and magnesium enhance the binding of ET-1 to both uterine membranes and cells. Calcium 31-38 endothelin-1 Oryctolagus cuniculus 76-80 1847589-9 1991 Conversely, divalent ions like calcium and magnesium enhance the binding of ET-1 to both uterine membranes and cells. Magnesium 43-52 endothelin-1 Oryctolagus cuniculus 76-80 2072872-0 1991 Ethanol causes vasoconstriction due to endothelin-1 release in rabbit gastric vessels. Ethanol 0-7 endothelin-1 Oryctolagus cuniculus 39-51 1649992-6 1991 Similarly, an increase in the basal coronary perfusion pressure by endothelin-1 (0.3 nM) to the same level as observed with L-NNA did not alter the vasomotor responses to ACh and sodium nitroprusside. Nitroarginine 124-129 endothelin-1 Oryctolagus cuniculus 67-79 2054955-0 1991 Endothelin-1 enhances vasoconstrictor responses to sympathetic nerve stimulation and noradrenaline in the rabbit ear artery. Norepinephrine 85-98 endothelin-1 Oryctolagus cuniculus 0-12 2054955-2 1991 In rabbit isolated perfused ear arteries denuded of endothelium, a low concentration of endothelin-1 (0.1 nmol/L) that had no direct vasoconstrictor action produced slowly developing enhancements of vasoconstrictor responses to noradrenaline and sympathetic nerve stimulation. Norepinephrine 228-241 endothelin-1 Oryctolagus cuniculus 88-100 1847589-2 1991 The staining was dramatically affected by subacute treatment with ovarian steroids: epithelial cells were predominantly positive in immature rabbits, whereas, in sex steroid-primed rabbits, ET-1 was mainly localized in the stromal compartment. Steroids 74-82 endothelin-1 Oryctolagus cuniculus 190-194 1725319-0 1991 Differential inhibitory action of phorbol-12,13-dibutyrate on the positive inotropic effect of endothelin-1 and Bay K 8644 in the isolated rabbit papillary muscle. Phorbol 12,13-Dibutyrate 34-58 endothelin-1 Oryctolagus cuniculus 95-107 2010389-3 1991 The pulmonary vasoconstrictor response to ET-1 and ET-3 was inhibited by intralobar infusion of nitrendipine but was not altered by indomethacin. Nitrendipine 96-108 endothelin-1 Oryctolagus cuniculus 42-55 1725343-2 1991 In low concentrations with no vasoconstrictor action (0.1 and 0.3 nM), and in a concentration that increased the perfusion pressure by 70 mm Hg (1 nM), ET-1 significantly enhanced responses to the agonists studied; this enhancement was apparently mediated by an increased influx of extracellular calcium through voltage-operated channels, as it was abolished by the calcium channel antagonist nicardipine (10 nM). Calcium 296-303 endothelin-1 Oryctolagus cuniculus 152-156 1725343-2 1991 In low concentrations with no vasoconstrictor action (0.1 and 0.3 nM), and in a concentration that increased the perfusion pressure by 70 mm Hg (1 nM), ET-1 significantly enhanced responses to the agonists studied; this enhancement was apparently mediated by an increased influx of extracellular calcium through voltage-operated channels, as it was abolished by the calcium channel antagonist nicardipine (10 nM). Nicardipine 393-404 endothelin-1 Oryctolagus cuniculus 152-156 1725343-3 1991 In contrast, higher concentrations of ET-1 (3 and 10 nM) inhibited responses to VP and 5-HT and this inhibitory effect was accentuated in the presence of nicardipine. Nicardipine 154-165 endothelin-1 Oryctolagus cuniculus 38-42 1725434-7 1991 Monocyclic Cys-Ser-Aoc-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp (Aoc = 8-aminooctanoic acid) exhibited the greatest binding affinity (1.0 to 1.6 microM) in all three tissues, but was still approximately 1,000-fold less effective than ET-1. aoc 19-22 endothelin-1 Oryctolagus cuniculus 233-237 1725434-7 1991 Monocyclic Cys-Ser-Aoc-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp (Aoc = 8-aminooctanoic acid) exhibited the greatest binding affinity (1.0 to 1.6 microM) in all three tissues, but was still approximately 1,000-fold less effective than ET-1. omega-aminocaprylic acid 70-90 endothelin-1 Oryctolagus cuniculus 233-237 2082715-7 1990 In the presence of endothelin-1, Ca2(+)-induced contractions were significantly blocked by nifedipine in the bladder but not in the urethra. Nifedipine 91-101 endothelin-1 Oryctolagus cuniculus 19-31 1725393-6 1991 Effects of ET-1 on the intracellular concentration of calcium of the cultured cells were examined with Indo-1. Calcium 54-61 endothelin-1 Oryctolagus cuniculus 11-15 20504726-3 1991 The vasoconstrictor action of endothelin-1 and its enhancing effect on stimulation-induced responses were significantly decreased by the presence of the dihydropyridine-type calcium channel antagonist nicardipine (10 nM). Nicardipine 201-212 endothelin-1 Oryctolagus cuniculus 30-42 2289525-0 1990 Endothelin-1 increases arterial sensitivity to 5-hydroxytryptamine. Serotonin 47-66 endothelin-1 Oryctolagus cuniculus 0-12 2289525-2 1990 ET-1 at concentrations of 0.4, 1 and 2 nM produced contractions of 7, 15 and 35% of maximal potassium chloride-induced contractions, respectively. Potassium Chloride 92-110 endothelin-1 Oryctolagus cuniculus 0-4 2201203-7 1990 Nicardipine (0.01-1 microM) produced concentration-dependent shifts in the ET-1 concentration-response curve in afferent arterioles. Nicardipine 0-11 endothelin-1 Oryctolagus cuniculus 75-79 2201203-8 1990 Verapamil (1 microM) also caused a significant shift in the ET-1 response curve. Verapamil 0-9 endothelin-1 Oryctolagus cuniculus 60-64 2201203-9 1990 The contractile response to ET-1 was significantly more sensitive to nicardipine than was the response to norepinephrine. Nicardipine 69-80 endothelin-1 Oryctolagus cuniculus 28-32 2263532-4 1990 Endothelin-1 released eicosanoids from a number of perfused isolated organ preparations. Eicosanoids 22-33 endothelin-1 Oryctolagus cuniculus 0-12 2263532-10 1990 Endothelin-1 and ET-3 injected into anesthetized rabbits inhibited ex vivo platelet aggregation by increasing cyclic AMP, presumably through the release of prostacyclin into the circulation. Cyclic AMP 110-120 endothelin-1 Oryctolagus cuniculus 0-12 2263532-10 1990 Endothelin-1 and ET-3 injected into anesthetized rabbits inhibited ex vivo platelet aggregation by increasing cyclic AMP, presumably through the release of prostacyclin into the circulation. Epoprostenol 156-168 endothelin-1 Oryctolagus cuniculus 0-12 1725319-3 1991 A tumor-promoting phorbol ester, i.e., phorbol-12,13-dibutyrate (PDBu), inhibited selectively the positive inotropic effect of ET-1 at the concentration that it did not (10 nmol/L) or only slightly (10-20% at 100 nmol/L) reduced the basal force of contraction and the positive inotropic effect of Bay K 8644. Phorbol Esters 18-31 endothelin-1 Oryctolagus cuniculus 127-131 1725319-3 1991 A tumor-promoting phorbol ester, i.e., phorbol-12,13-dibutyrate (PDBu), inhibited selectively the positive inotropic effect of ET-1 at the concentration that it did not (10 nmol/L) or only slightly (10-20% at 100 nmol/L) reduced the basal force of contraction and the positive inotropic effect of Bay K 8644. Phorbol 12,13-Dibutyrate 39-63 endothelin-1 Oryctolagus cuniculus 127-131 1725319-3 1991 A tumor-promoting phorbol ester, i.e., phorbol-12,13-dibutyrate (PDBu), inhibited selectively the positive inotropic effect of ET-1 at the concentration that it did not (10 nmol/L) or only slightly (10-20% at 100 nmol/L) reduced the basal force of contraction and the positive inotropic effect of Bay K 8644. Phorbol 12,13-Dibutyrate 65-69 endothelin-1 Oryctolagus cuniculus 127-131 1725319-3 1991 A tumor-promoting phorbol ester, i.e., phorbol-12,13-dibutyrate (PDBu), inhibited selectively the positive inotropic effect of ET-1 at the concentration that it did not (10 nmol/L) or only slightly (10-20% at 100 nmol/L) reduced the basal force of contraction and the positive inotropic effect of Bay K 8644. bay k 297-302 endothelin-1 Oryctolagus cuniculus 127-131 1725319-4 1991 The positive inotropic effect of 10 mumol/L of phenylephrine mediated via myocardial alpha 1-adrenoceptors (in the presence of 0.3 mumol/L of bupranolol) was likewise inhibited by PDBu in the same concentration range as it suppressed the ET-1-induced positive inotropic effect. Phenylephrine 47-60 endothelin-1 Oryctolagus cuniculus 238-242 1725319-7 1991 The present findings indicate that ET-1 elicits a positive inotropic effect on the rabbit ventricular myocardium, the characteristics of which are similar to those of myocardial alpha-adrenoceptor activation, which may involve the phosphoinositide hydrolysis. Phosphatidylinositols 231-247 endothelin-1 Oryctolagus cuniculus 35-39 2183912-0 1990 Endothelin-1 inhibits platelet aggregation in vivo: a study with 111indium-labelled platelets. Indium-111 65-74 endothelin-1 Oryctolagus cuniculus 0-12 2183912-15 1990 abolished the ET-1-induced inhibition of ADP-stimulated platelet aggregation and significantly potentiated and prolonged the pressor response brought about by ET-1. Adenosine Diphosphate 41-44 endothelin-1 Oryctolagus cuniculus 14-18 2183912-17 1990 In conclusion, the data demonstrate that ET-1 potently inhibits platelet aggregation in the anaesthetized rabbit in vivo by releasing a hypotensive and anti-aggregatory cyclo-oxygenase product, presumably prostacyclin, into the circulation. Epoprostenol 205-217 endothelin-1 Oryctolagus cuniculus 41-45 26640529-6 2015 XMJ was observed to decrease the blood lipid levels of the AS rabbits; increase the concentration of high-density lipoprotein and apolipoprotein A; decrease blood viscosity, erythrocyte sedimentation rate and hematocrit; elevate the levels of endothelial nitric oxide synthase (eNOS) and Na+/H+ exchanger 1 in vascular tissues and decrease the levels of angiotensin II receptor, type 1 (AT-1) and endothelin-1 (ET-1). 4-[[(1E)-2-(4-CHLOROPHENYL)ETHENYL]SULFONYL]-1-[[1-(4-PYRIDINYL)-4-PIPERIDINYL]METHYL]PIPERAZINONE 0-3 endothelin-1 Oryctolagus cuniculus 397-409