PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 33250374-1 2021 SLC5A7 encodes the presynaptic sodium-dependant high-affinity choline transporter 1 (CHT), which uptakes choline to the presynaptic nerve terminal following the breakdown of acetylcholine by the acetylcholinesterase within the synaptic cleft. Choline 62-69 solute carrier family 5 member 7 Homo sapiens 85-88 33640406-1 2021 The high-affinity choline transporter CHT 1 mediates choline uptake, the rate-limiting and regulatory step in acetylcholine synthesis at cholinergic presynaptic terminals. Choline 18-25 solute carrier family 5 member 7 Homo sapiens 38-43 33640406-1 2021 The high-affinity choline transporter CHT 1 mediates choline uptake, the rate-limiting and regulatory step in acetylcholine synthesis at cholinergic presynaptic terminals. Choline 53-60 solute carrier family 5 member 7 Homo sapiens 38-43 33640406-1 2021 The high-affinity choline transporter CHT 1 mediates choline uptake, the rate-limiting and regulatory step in acetylcholine synthesis at cholinergic presynaptic terminals. Acetylcholine 110-123 solute carrier family 5 member 7 Homo sapiens 38-43 33640406-2 2021 CHT1-medated choline uptake is specifically inhibited by hemicholinium-3, which is a type of choline analog that acts as a competitive inhibitor. Choline 13-20 solute carrier family 5 member 7 Homo sapiens 0-4 33640406-2 2021 CHT1-medated choline uptake is specifically inhibited by hemicholinium-3, which is a type of choline analog that acts as a competitive inhibitor. Hemicholinium 3 57-72 solute carrier family 5 member 7 Homo sapiens 0-4 33640406-2 2021 CHT1-medated choline uptake is specifically inhibited by hemicholinium-3, which is a type of choline analog that acts as a competitive inhibitor. Choline 93-100 solute carrier family 5 member 7 Homo sapiens 0-4 33640406-3 2021 Although the substrate choline and the inhibitor hemicholinium-3 are well-established ligands of CHT 1, few potent ligands other than choline analogs have been reported. Choline 23-30 solute carrier family 5 member 7 Homo sapiens 97-102 33640406-3 2021 Although the substrate choline and the inhibitor hemicholinium-3 are well-established ligands of CHT 1, few potent ligands other than choline analogs have been reported. Hemicholinium 3 49-64 solute carrier family 5 member 7 Homo sapiens 97-102 33640406-4 2021 Here we show that tetrahydropyrimidine anthelmintics, known as nicotinic acetylcholine receptor agonists, act as competitive inhibitors of CHT 1. tetrahydropyrimidine 18-38 solute carrier family 5 member 7 Homo sapiens 139-144 33640406-4 2021 Here we show that tetrahydropyrimidine anthelmintics, known as nicotinic acetylcholine receptor agonists, act as competitive inhibitors of CHT 1. Acetylcholine 73-86 solute carrier family 5 member 7 Homo sapiens 139-144 33640406-6 2021 We found that morantel as well as other tetrahydropyrimidines, pyrantel and oxantel, potently inhibits the high-affinity choline uptake activity of CHT 1 in a competitive manner similar to the inhibitor hemicholinium-3. Morantel 14-22 solute carrier family 5 member 7 Homo sapiens 148-153 33640406-6 2021 We found that morantel as well as other tetrahydropyrimidines, pyrantel and oxantel, potently inhibits the high-affinity choline uptake activity of CHT 1 in a competitive manner similar to the inhibitor hemicholinium-3. tetrahydropyrimidines 40-61 solute carrier family 5 member 7 Homo sapiens 148-153 33640406-6 2021 We found that morantel as well as other tetrahydropyrimidines, pyrantel and oxantel, potently inhibits the high-affinity choline uptake activity of CHT 1 in a competitive manner similar to the inhibitor hemicholinium-3. Pyrantel 63-71 solute carrier family 5 member 7 Homo sapiens 148-153 33640406-6 2021 We found that morantel as well as other tetrahydropyrimidines, pyrantel and oxantel, potently inhibits the high-affinity choline uptake activity of CHT 1 in a competitive manner similar to the inhibitor hemicholinium-3. oxantel 76-83 solute carrier family 5 member 7 Homo sapiens 148-153 33640406-6 2021 We found that morantel as well as other tetrahydropyrimidines, pyrantel and oxantel, potently inhibits the high-affinity choline uptake activity of CHT 1 in a competitive manner similar to the inhibitor hemicholinium-3. Choline 121-128 solute carrier family 5 member 7 Homo sapiens 148-153 33640406-6 2021 We found that morantel as well as other tetrahydropyrimidines, pyrantel and oxantel, potently inhibits the high-affinity choline uptake activity of CHT 1 in a competitive manner similar to the inhibitor hemicholinium-3. Hemicholinium 3 203-218 solute carrier family 5 member 7 Homo sapiens 148-153 33250374-0 2021 Presynaptic congenital myasthenic syndrome due to three novel mutations in SLC5A7 encoding the sodium-dependant high-affinity choline transporter. Sodium 95-101 solute carrier family 5 member 7 Homo sapiens 75-81 33250374-0 2021 Presynaptic congenital myasthenic syndrome due to three novel mutations in SLC5A7 encoding the sodium-dependant high-affinity choline transporter. Choline 126-133 solute carrier family 5 member 7 Homo sapiens 75-81 33250374-1 2021 SLC5A7 encodes the presynaptic sodium-dependant high-affinity choline transporter 1 (CHT), which uptakes choline to the presynaptic nerve terminal following the breakdown of acetylcholine by the acetylcholinesterase within the synaptic cleft. Sodium 31-37 solute carrier family 5 member 7 Homo sapiens 0-6 33250374-1 2021 SLC5A7 encodes the presynaptic sodium-dependant high-affinity choline transporter 1 (CHT), which uptakes choline to the presynaptic nerve terminal following the breakdown of acetylcholine by the acetylcholinesterase within the synaptic cleft. Sodium 31-37 solute carrier family 5 member 7 Homo sapiens 48-83 33250374-1 2021 SLC5A7 encodes the presynaptic sodium-dependant high-affinity choline transporter 1 (CHT), which uptakes choline to the presynaptic nerve terminal following the breakdown of acetylcholine by the acetylcholinesterase within the synaptic cleft. Sodium 31-37 solute carrier family 5 member 7 Homo sapiens 85-88 33250374-1 2021 SLC5A7 encodes the presynaptic sodium-dependant high-affinity choline transporter 1 (CHT), which uptakes choline to the presynaptic nerve terminal following the breakdown of acetylcholine by the acetylcholinesterase within the synaptic cleft. Choline 62-69 solute carrier family 5 member 7 Homo sapiens 0-6 33250374-1 2021 SLC5A7 encodes the presynaptic sodium-dependant high-affinity choline transporter 1 (CHT), which uptakes choline to the presynaptic nerve terminal following the breakdown of acetylcholine by the acetylcholinesterase within the synaptic cleft. Acetylcholine 174-187 solute carrier family 5 member 7 Homo sapiens 0-6 33250374-1 2021 SLC5A7 encodes the presynaptic sodium-dependant high-affinity choline transporter 1 (CHT), which uptakes choline to the presynaptic nerve terminal following the breakdown of acetylcholine by the acetylcholinesterase within the synaptic cleft. Acetylcholine 174-187 solute carrier family 5 member 7 Homo sapiens 48-83 33250374-1 2021 SLC5A7 encodes the presynaptic sodium-dependant high-affinity choline transporter 1 (CHT), which uptakes choline to the presynaptic nerve terminal following the breakdown of acetylcholine by the acetylcholinesterase within the synaptic cleft. Acetylcholine 174-187 solute carrier family 5 member 7 Homo sapiens 85-88 32662986-6 2020 Among them, CC, CCNK, and HCHT had both ACE inhibitory activity (IC50 values were 9.81, 9.00 and 114.99 muM respectively) and antioxidant activity (strong DPPH and ABTS free radical scavenging activities). 1,1-diphenyl-2-picrylhydrazyl 155-159 solute carrier family 5 member 7 Homo sapiens 26-30 32662986-6 2020 Among them, CC, CCNK, and HCHT had both ACE inhibitory activity (IC50 values were 9.81, 9.00 and 114.99 muM respectively) and antioxidant activity (strong DPPH and ABTS free radical scavenging activities). 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid 164-168 solute carrier family 5 member 7 Homo sapiens 26-30 32662986-9 2020 Experiments with damaged HepG2 cells showed that peptides CC, CCNK, and HCHT have antioxidant activity through their cytoprotective effects and by reducing the reactive oxygen species (ROS) content. Reactive Oxygen Species 160-183 solute carrier family 5 member 7 Homo sapiens 72-76 32662986-9 2020 Experiments with damaged HepG2 cells showed that peptides CC, CCNK, and HCHT have antioxidant activity through their cytoprotective effects and by reducing the reactive oxygen species (ROS) content. Reactive Oxygen Species 185-188 solute carrier family 5 member 7 Homo sapiens 72-76 31761205-1 2020 Polymer blend made from poly(epsilon - caprolactone)/chitosan (PCL/CHT) offers interesting opportunities for biological applications. Chitosan 53-61 solute carrier family 5 member 7 Homo sapiens 67-70 31761205-1 2020 Polymer blend made from poly(epsilon - caprolactone)/chitosan (PCL/CHT) offers interesting opportunities for biological applications. Polymers 0-7 solute carrier family 5 member 7 Homo sapiens 67-70 31761205-1 2020 Polymer blend made from poly(epsilon - caprolactone)/chitosan (PCL/CHT) offers interesting opportunities for biological applications. polycaprolactone 24-52 solute carrier family 5 member 7 Homo sapiens 67-70 31761205-4 2020 The PCL/CHT membranes are fully characterized in term of physico-chemical properties (ATR-FTIR, XRD and DSC) to understand the miscibility of the two-polymer blend. Polymers 150-157 solute carrier family 5 member 7 Homo sapiens 8-11 32640966-9 2020 RA induced the highest number of CHT1-immunoreactive cells while ST- and BDNF-differentiation reduced CHT1-immunoreactive cells, indicating a decrease in the cholinergic phenotype. Tretinoin 0-2 solute carrier family 5 member 7 Homo sapiens 33-37 32640966-6 2020 Undifferentiated cells expressed low levels of tyrosine hydroxylase (TH) and higher levels of the high-affinity choline transporter (CHT1). Choline 112-119 solute carrier family 5 member 7 Homo sapiens 133-137 32640966-11 2020 Ascorbic acid increased the number of CHT1-immunoreactive cells in all differentiation procedures and ST-differentiated TH-positive cells significantly. Ascorbic Acid 0-13 solute carrier family 5 member 7 Homo sapiens 38-42 31483434-4 2019 A new fluorophore bearing two isophorone malononitrile structures was conjugated with DNBS to provide a target probe (CHT), which exhibited a ratiometric sensing behavior towards biothiols. biothiols 179-188 solute carrier family 5 member 7 Homo sapiens 118-121 31483434-6 2019 Most importantly, CHT has high stability in the quantitative detection of Cys compared to the control probe CHM, which performed an "off-on" sensing for biothiols. Cysteine 74-77 solute carrier family 5 member 7 Homo sapiens 18-21 31483434-4 2019 A new fluorophore bearing two isophorone malononitrile structures was conjugated with DNBS to provide a target probe (CHT), which exhibited a ratiometric sensing behavior towards biothiols. isophorone 30-40 solute carrier family 5 member 7 Homo sapiens 118-121 31483434-6 2019 Most importantly, CHT has high stability in the quantitative detection of Cys compared to the control probe CHM, which performed an "off-on" sensing for biothiols. biothiols 153-162 solute carrier family 5 member 7 Homo sapiens 18-21 31483434-4 2019 A new fluorophore bearing two isophorone malononitrile structures was conjugated with DNBS to provide a target probe (CHT), which exhibited a ratiometric sensing behavior towards biothiols. dicyanmethane 41-54 solute carrier family 5 member 7 Homo sapiens 118-121 31483434-7 2019 Endogenous biothiols were successfully monitored with CHT in live cells through the ratiometric fluorescence signal. biothiols 11-20 solute carrier family 5 member 7 Homo sapiens 54-57 30681159-8 2019 This mini review will provide a summary of the biochemical pathways, in which choline is involved and their respective inborn errors of metabolism (caused by mutations in SLC5A7, CHAT, SLC44A1, CHKB, PCYT1A, CEPT1, CAD; DHODH, UMPS, FMO3, DMGDH, and GNMT). Choline 78-85 solute carrier family 5 member 7 Homo sapiens 171-177 31483434-4 2019 A new fluorophore bearing two isophorone malononitrile structures was conjugated with DNBS to provide a target probe (CHT), which exhibited a ratiometric sensing behavior towards biothiols. 2,4-dinitrofluorobenzene sulfonic acid 86-90 solute carrier family 5 member 7 Homo sapiens 118-121 29987464-1 2019 The present paper investigates the efficiency of coagulation/flocculation process using aluminum sulfate as coagulant and CHT industrial flocculent as coagulant aid/flocculent in the treatment of vegetable oil refinery wastewater (VORW). Plant Oils 196-209 solute carrier family 5 member 7 Homo sapiens 122-125 31299140-2 2019 A central component of the NMJ is the sodium-dependent high-affinity choline transporter 1 (CHT1), a solute carrier protein (gene symbol SLC5A7), responsible for the reuptake of choline into nerve termini has recently been implicated as one of several autosomal recessive causes of CMS. Sodium 38-44 solute carrier family 5 member 7 Homo sapiens 55-90 31299140-2 2019 A central component of the NMJ is the sodium-dependent high-affinity choline transporter 1 (CHT1), a solute carrier protein (gene symbol SLC5A7), responsible for the reuptake of choline into nerve termini has recently been implicated as one of several autosomal recessive causes of CMS. Sodium 38-44 solute carrier family 5 member 7 Homo sapiens 92-96 31299140-2 2019 A central component of the NMJ is the sodium-dependent high-affinity choline transporter 1 (CHT1), a solute carrier protein (gene symbol SLC5A7), responsible for the reuptake of choline into nerve termini has recently been implicated as one of several autosomal recessive causes of CMS. Sodium 38-44 solute carrier family 5 member 7 Homo sapiens 137-143 31299140-2 2019 A central component of the NMJ is the sodium-dependent high-affinity choline transporter 1 (CHT1), a solute carrier protein (gene symbol SLC5A7), responsible for the reuptake of choline into nerve termini has recently been implicated as one of several autosomal recessive causes of CMS. Choline 69-76 solute carrier family 5 member 7 Homo sapiens 92-96 31299140-2 2019 A central component of the NMJ is the sodium-dependent high-affinity choline transporter 1 (CHT1), a solute carrier protein (gene symbol SLC5A7), responsible for the reuptake of choline into nerve termini has recently been implicated as one of several autosomal recessive causes of CMS. Choline 69-76 solute carrier family 5 member 7 Homo sapiens 137-143 31547050-6 2019 They include (I) polyspecific organic cation transporters (OCT1-3: SLC22A1-3) with a low affinity for choline, (II) high-affinity choline transporter 1 (CHT1: SLC5A7), and (III) choline transporter-like proteins (CTL1-5: SLC44A1-5). Choline 102-109 solute carrier family 5 member 7 Homo sapiens 153-157 31547050-6 2019 They include (I) polyspecific organic cation transporters (OCT1-3: SLC22A1-3) with a low affinity for choline, (II) high-affinity choline transporter 1 (CHT1: SLC5A7), and (III) choline transporter-like proteins (CTL1-5: SLC44A1-5). Choline 130-137 solute carrier family 5 member 7 Homo sapiens 153-157 31547050-6 2019 They include (I) polyspecific organic cation transporters (OCT1-3: SLC22A1-3) with a low affinity for choline, (II) high-affinity choline transporter 1 (CHT1: SLC5A7), and (III) choline transporter-like proteins (CTL1-5: SLC44A1-5). Choline 130-137 solute carrier family 5 member 7 Homo sapiens 159-165 31547050-6 2019 They include (I) polyspecific organic cation transporters (OCT1-3: SLC22A1-3) with a low affinity for choline, (II) high-affinity choline transporter 1 (CHT1: SLC5A7), and (III) choline transporter-like proteins (CTL1-5: SLC44A1-5). Choline 130-137 solute carrier family 5 member 7 Homo sapiens 153-157 31547050-6 2019 They include (I) polyspecific organic cation transporters (OCT1-3: SLC22A1-3) with a low affinity for choline, (II) high-affinity choline transporter 1 (CHT1: SLC5A7), and (III) choline transporter-like proteins (CTL1-5: SLC44A1-5). Choline 130-137 solute carrier family 5 member 7 Homo sapiens 159-165 30131010-6 2019 Concurrent and adjuvant temozolomide chemotherapy (TMZ-CHT) was administered in 7 (23.3%) and 11 (40.7%) patients received only adjuvant TMZ-CHT. Temozolomide 24-36 solute carrier family 5 member 7 Homo sapiens 55-58 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Adenosine 51-60 solute carrier family 5 member 7 Homo sapiens 194-199 29874875-3 2018 CMS due to mutations in SLC5A7 and SLC18A3, impairing the synthesis and recycling of acetylcholine, have recently been described. Acetylcholine 85-98 solute carrier family 5 member 7 Homo sapiens 24-30 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Adenosine 51-60 solute carrier family 5 member 7 Homo sapiens 324-329 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Guanosine 86-95 solute carrier family 5 member 7 Homo sapiens 194-199 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Guanosine 86-95 solute carrier family 5 member 7 Homo sapiens 324-329 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Cytidine 120-128 solute carrier family 5 member 7 Homo sapiens 194-199 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Cytidine 120-128 solute carrier family 5 member 7 Homo sapiens 324-329 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Guanosine 129-138 solute carrier family 5 member 7 Homo sapiens 194-199 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Guanosine 129-138 solute carrier family 5 member 7 Homo sapiens 324-329 30172469-3 2018 One of these syndromes is caused by the impairment of the presynaptic sodium-dependent high-affinity choline transporter 1, as a result of a mutation of the SCL5A7 gene associated with congenital myasthenic syndrome type 20 (MIM # 617143). Sodium 70-76 solute carrier family 5 member 7 Homo sapiens 87-122 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Cytidine 120-128 solute carrier family 5 member 7 Homo sapiens 194-199 28577989-0 2017 Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity. Hemicholinium 3 0-15 solute carrier family 5 member 7 Homo sapiens 106-125 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Cytidine 120-128 solute carrier family 5 member 7 Homo sapiens 324-329 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Cytidine 120-128 solute carrier family 5 member 7 Homo sapiens 194-199 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Cytidine 120-128 solute carrier family 5 member 7 Homo sapiens 324-329 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Cytidine 120-128 solute carrier family 5 member 7 Homo sapiens 194-199 29087477-8 2018 In ESI-MS measurements of the solutions containing adenosine, cytidine, thymidine and guanosine, we observed protonated cytidine-guanosine dimer (CH+-G) and protonated cytidine-thymidine dimer (CH+-T) in addition to hemiprotonated cytidine-cytidine dimer (CH+-C) with following relative peak height, (CH+-C) > (CH+-G) (CH+-T) > (CH+-A). Cytidine 120-128 solute carrier family 5 member 7 Homo sapiens 324-329 29163036-3 2017 The high-affinity choline transporter (CHT) is present at the presynaptic cholinergic nerve terminal and is responsible for the reuptake of choline produced by hydrolysis of ACh following its release. Choline 18-25 solute carrier family 5 member 7 Homo sapiens 39-42 29163036-3 2017 The high-affinity choline transporter (CHT) is present at the presynaptic cholinergic nerve terminal and is responsible for the reuptake of choline produced by hydrolysis of ACh following its release. Acetylcholine 174-177 solute carrier family 5 member 7 Homo sapiens 18-37 29163036-3 2017 The high-affinity choline transporter (CHT) is present at the presynaptic cholinergic nerve terminal and is responsible for the reuptake of choline produced by hydrolysis of ACh following its release. Acetylcholine 174-177 solute carrier family 5 member 7 Homo sapiens 39-42 29163036-4 2017 Disruption of CHT function leads to decreased choline uptake and ACh synthesis, leading to impaired cholinergic neurotransmission. Choline 46-53 solute carrier family 5 member 7 Homo sapiens 14-17 29163036-4 2017 Disruption of CHT function leads to decreased choline uptake and ACh synthesis, leading to impaired cholinergic neurotransmission. Acetylcholine 65-68 solute carrier family 5 member 7 Homo sapiens 14-17 28836753-3 2017 Here, a series of 1% Ga2O3-containing mesoporous bioactive glass-chitosan composite scaffolds (Ga-MBG/CHT) were constructed by the lyophilization process and the effect of various concentrations of Ga-MBG (10, 30, and 50 wt %) on the hemostatic function of the CHT scaffold was assessed as compared to that of Celox Rapid gauze (CXR), a current commercially available chitosan-coated hemostatic gauze. ga2o3 21-26 solute carrier family 5 member 7 Homo sapiens 95-105 28577989-0 2017 Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity. Hemicholinium 3 0-15 solute carrier family 5 member 7 Homo sapiens 127-130 28577989-0 2017 Hemicholinium-3 sensitive choline transport in human T lymphocytes: Evidence for use as a proxy for brain choline transporter (CHT) capacity. Choline 26-33 solute carrier family 5 member 7 Homo sapiens 106-125 28577989-1 2017 The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. Choline 23-30 solute carrier family 5 member 7 Homo sapiens 80-99 28577989-1 2017 The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. Choline 23-30 solute carrier family 5 member 7 Homo sapiens 101-104 28577989-1 2017 The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. Acetylcholine 173-186 solute carrier family 5 member 7 Homo sapiens 80-99 28577989-1 2017 The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. Acetylcholine 173-186 solute carrier family 5 member 7 Homo sapiens 101-104 28577989-1 2017 The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. Acetylcholine 188-191 solute carrier family 5 member 7 Homo sapiens 80-99 28577989-1 2017 The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. Acetylcholine 188-191 solute carrier family 5 member 7 Homo sapiens 101-104 28577989-3 2017 Next, we demonstrated CHT-mediated choline transport in human T cells. Choline 35-42 solute carrier family 5 member 7 Homo sapiens 22-25 28649946-0 2017 Choline Transporter Immunohistochemistry: An Effective Substitute for Acetylcholinesterase Histochemistry to Diagnose Hirschsprung Disease With Formalin-fixed Paraffin-embedded Rectal Biopsies. Formaldehyde 144-152 solute carrier family 5 member 7 Homo sapiens 0-19 28649946-0 2017 Choline Transporter Immunohistochemistry: An Effective Substitute for Acetylcholinesterase Histochemistry to Diagnose Hirschsprung Disease With Formalin-fixed Paraffin-embedded Rectal Biopsies. Paraffin 159-167 solute carrier family 5 member 7 Homo sapiens 0-19 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Choline 14-21 solute carrier family 5 member 7 Homo sapiens 151-170 28584264-2 2017 The human macrophage chitotriosidase (HCHT) is a chitinase that hydrolyses glycosidic bonds between the N-acetyl-D-glucosamine units of this biopolymer. Acetylglucosamine 104-126 solute carrier family 5 member 7 Homo sapiens 38-42 28584264-3 2017 HCHT belongs to the Glycoside Hydrolase (GH) superfamily and contains a well-characterized catalytic domain appended to a chitin-binding domain (ChBDCHIT1). Chitin 122-128 solute carrier family 5 member 7 Homo sapiens 0-4 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Choline 14-21 solute carrier family 5 member 7 Homo sapiens 172-175 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Choline 14-21 solute carrier family 5 member 7 Homo sapiens 192-198 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Acetylcholine 41-54 solute carrier family 5 member 7 Homo sapiens 151-170 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Acetylcholine 41-54 solute carrier family 5 member 7 Homo sapiens 172-175 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Acetylcholine 41-54 solute carrier family 5 member 7 Homo sapiens 192-198 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Acetylcholine 56-59 solute carrier family 5 member 7 Homo sapiens 151-170 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Acetylcholine 56-59 solute carrier family 5 member 7 Homo sapiens 172-175 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Acetylcholine 56-59 solute carrier family 5 member 7 Homo sapiens 192-198 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Sodium 112-118 solute carrier family 5 member 7 Homo sapiens 151-170 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Sodium 112-118 solute carrier family 5 member 7 Homo sapiens 172-175 28289374-3 2017 High affinity choline uptake (HACU) into acetylcholine (ACh)-synthesizing neurons is critically mediated by the sodium- and pH-dependent high-affinity choline transporter (CHT, encoded by the SLC5A7 gene). Sodium 112-118 solute carrier family 5 member 7 Homo sapiens 192-198 27404793-3 2016 The neuronal choline transporter (CHT) strongly influences the synthesis and release of acetylcholine (ACh). Acetylcholine 88-101 solute carrier family 5 member 7 Homo sapiens 13-32 28129403-3 2017 The catalytic domain of HCHT is connected to the carbohydrate binding module (CBM) through a flexible hinge region. Carbohydrates 49-61 solute carrier family 5 member 7 Homo sapiens 24-28 28129403-7 2017 Although the studies on soluble chitin derivatives suggest the endo-character of HCHT, the mode of action of the enzyme on crystalline chitin is not known. Chitin 32-38 solute carrier family 5 member 7 Homo sapiens 81-85 28129403-8 2017 Here, we made a thorough characterization of HCHT in terms of the mode of action, processivity, binding, and rate constants for the catalysis and dissociation using alpha-chitin as substrate. alpha-chitin 165-177 solute carrier family 5 member 7 Homo sapiens 45-49 28129403-9 2017 HCHT efficiently released the end-label from reducing-end labelled chitin and had also high probability (95%) of endo-mode initiation of processive run. Chitin 67-73 solute carrier family 5 member 7 Homo sapiens 0-4 27575469-0 2016 Optimization of the choline transporter (CHT) inhibitor ML352: Development of VU6001221, an improved in vivo tool compound. ML352 56-61 solute carrier family 5 member 7 Homo sapiens 20-39 27575469-0 2016 Optimization of the choline transporter (CHT) inhibitor ML352: Development of VU6001221, an improved in vivo tool compound. VU6001221 78-87 solute carrier family 5 member 7 Homo sapiens 20-39 27569547-3 2016 Whole-exome sequencing and Sanger sequencing in six unrelated families identified compound heterozygous and homozygous mutations in SLC5A7 encoding the presynaptic sodium-dependent high-affinity choline transporter 1 (CHT), which is known to be mutated in one dominant form of distal motor neuronopathy (DHMN7A). Sodium 164-170 solute carrier family 5 member 7 Homo sapiens 132-138 27569547-3 2016 Whole-exome sequencing and Sanger sequencing in six unrelated families identified compound heterozygous and homozygous mutations in SLC5A7 encoding the presynaptic sodium-dependent high-affinity choline transporter 1 (CHT), which is known to be mutated in one dominant form of distal motor neuronopathy (DHMN7A). Sodium 164-170 solute carrier family 5 member 7 Homo sapiens 218-221 27404793-3 2016 The neuronal choline transporter (CHT) strongly influences the synthesis and release of acetylcholine (ACh). Acetylcholine 88-101 solute carrier family 5 member 7 Homo sapiens 34-37 27404793-3 2016 The neuronal choline transporter (CHT) strongly influences the synthesis and release of acetylcholine (ACh). Acetylcholine 103-106 solute carrier family 5 member 7 Homo sapiens 13-32 27404793-3 2016 The neuronal choline transporter (CHT) strongly influences the synthesis and release of acetylcholine (ACh). Acetylcholine 103-106 solute carrier family 5 member 7 Homo sapiens 34-37 27404793-4 2016 As the capacity of the CHT to import choline into the neuron is a major, presynaptic determinant of cholinergic neuromodulation, we hypothesize that genetically-imposed CHT capacity variation impacts the balance of bottom-up versus top-down control of visual attention. Choline 37-44 solute carrier family 5 member 7 Homo sapiens 23-26 27404793-4 2016 As the capacity of the CHT to import choline into the neuron is a major, presynaptic determinant of cholinergic neuromodulation, we hypothesize that genetically-imposed CHT capacity variation impacts the balance of bottom-up versus top-down control of visual attention. Choline 37-44 solute carrier family 5 member 7 Homo sapiens 169-172 27288078-0 2016 Choline on the Move: Perspectives on the Molecular Physiology and Pharmacology of the Presynaptic Choline Transporter. Choline 0-7 solute carrier family 5 member 7 Homo sapiens 98-117 27059281-3 2016 Herein, the use of cellulose nanocrystals (CNCs) as reinforcing nanofillers in aligned electrospun scaffolds based on a natural/synthetic polymer blend matrix, poly-epsilon-caprolactone/chitosan (PCL/CHT) is reported. Cellulose 19-28 solute carrier family 5 member 7 Homo sapiens 200-203 27288078-2 2016 High-affinity choline uptake (HACU) was one of the last features of cholinergic signaling to be defined at a molecular level, achieved through the cloning of the choline transporter (CHT, SLC5A7). Choline 14-21 solute carrier family 5 member 7 Homo sapiens 162-181 27288078-2 2016 High-affinity choline uptake (HACU) was one of the last features of cholinergic signaling to be defined at a molecular level, achieved through the cloning of the choline transporter (CHT, SLC5A7). Choline 14-21 solute carrier family 5 member 7 Homo sapiens 183-186 27288078-2 2016 High-affinity choline uptake (HACU) was one of the last features of cholinergic signaling to be defined at a molecular level, achieved through the cloning of the choline transporter (CHT, SLC5A7). Choline 14-21 solute carrier family 5 member 7 Homo sapiens 188-194 27288078-3 2016 In retrospect, the molecular era of CHT studies initiated with the identification of hemicholinium-3 (HC-3), a potent, competitive CHT antagonist, though it would take another 30 years before HC-3, in radiolabeled form, was used by Joseph Coyle"s laboratory to identify and monitor the dynamics of CHT proteins. Hemicholinium 3 85-100 solute carrier family 5 member 7 Homo sapiens 36-39 27288078-3 2016 In retrospect, the molecular era of CHT studies initiated with the identification of hemicholinium-3 (HC-3), a potent, competitive CHT antagonist, though it would take another 30 years before HC-3, in radiolabeled form, was used by Joseph Coyle"s laboratory to identify and monitor the dynamics of CHT proteins. Hemicholinium 3 85-100 solute carrier family 5 member 7 Homo sapiens 131-134 27288078-3 2016 In retrospect, the molecular era of CHT studies initiated with the identification of hemicholinium-3 (HC-3), a potent, competitive CHT antagonist, though it would take another 30 years before HC-3, in radiolabeled form, was used by Joseph Coyle"s laboratory to identify and monitor the dynamics of CHT proteins. Hemicholinium 3 85-100 solute carrier family 5 member 7 Homo sapiens 131-134 27288078-3 2016 In retrospect, the molecular era of CHT studies initiated with the identification of hemicholinium-3 (HC-3), a potent, competitive CHT antagonist, though it would take another 30 years before HC-3, in radiolabeled form, was used by Joseph Coyle"s laboratory to identify and monitor the dynamics of CHT proteins. Hydrocortisone 102-104 solute carrier family 5 member 7 Homo sapiens 36-39 27288078-3 2016 In retrospect, the molecular era of CHT studies initiated with the identification of hemicholinium-3 (HC-3), a potent, competitive CHT antagonist, though it would take another 30 years before HC-3, in radiolabeled form, was used by Joseph Coyle"s laboratory to identify and monitor the dynamics of CHT proteins. Hydrocortisone 102-104 solute carrier family 5 member 7 Homo sapiens 131-134 27288078-3 2016 In retrospect, the molecular era of CHT studies initiated with the identification of hemicholinium-3 (HC-3), a potent, competitive CHT antagonist, though it would take another 30 years before HC-3, in radiolabeled form, was used by Joseph Coyle"s laboratory to identify and monitor the dynamics of CHT proteins. Hydrocortisone 102-104 solute carrier family 5 member 7 Homo sapiens 131-134 26813123-4 2016 ACh present at the synaptic cleft is promptly hydrolyzed by the enzyme acetylcholinesterase (AChE), forming acetate and choline, which is recycled into the presynaptic nerve terminal by the high-affinity choline transporter (CHT1). Acetylcholine 0-3 solute carrier family 5 member 7 Homo sapiens 225-229 26813123-4 2016 ACh present at the synaptic cleft is promptly hydrolyzed by the enzyme acetylcholinesterase (AChE), forming acetate and choline, which is recycled into the presynaptic nerve terminal by the high-affinity choline transporter (CHT1). Acetates 108-115 solute carrier family 5 member 7 Homo sapiens 225-229 26813123-4 2016 ACh present at the synaptic cleft is promptly hydrolyzed by the enzyme acetylcholinesterase (AChE), forming acetate and choline, which is recycled into the presynaptic nerve terminal by the high-affinity choline transporter (CHT1). Choline 77-84 solute carrier family 5 member 7 Homo sapiens 225-229 26813123-4 2016 ACh present at the synaptic cleft is promptly hydrolyzed by the enzyme acetylcholinesterase (AChE), forming acetate and choline, which is recycled into the presynaptic nerve terminal by the high-affinity choline transporter (CHT1). Choline 120-127 solute carrier family 5 member 7 Homo sapiens 225-229 25073461-2 2014 CHT1 is present on the presynaptic terminal of cholinergic neurons, and takes up choline which is the precursor of acetylcholine. Choline 47-54 solute carrier family 5 member 7 Homo sapiens 0-4 26793864-9 2015 The use of CHT resulted in a decrease of AMD release only at pH 6.8 in formulations with the lowest concentration of KOL. kol 117-120 solute carrier family 5 member 7 Homo sapiens 11-14 25970623-1 2015 The high-affinity choline transporter (CHT) is responsible for choline uptake into cholinergic neurons, with this being the rate-limiting step for acetylcholine production. Choline 18-25 solute carrier family 5 member 7 Homo sapiens 39-42 25970623-1 2015 The high-affinity choline transporter (CHT) is responsible for choline uptake into cholinergic neurons, with this being the rate-limiting step for acetylcholine production. Acetylcholine 147-160 solute carrier family 5 member 7 Homo sapiens 18-37 25970623-1 2015 The high-affinity choline transporter (CHT) is responsible for choline uptake into cholinergic neurons, with this being the rate-limiting step for acetylcholine production. Acetylcholine 147-160 solute carrier family 5 member 7 Homo sapiens 39-42 25970623-2 2015 Altering CHT protein disposition directly impacts choline uptake activity and cholinergic neurotransmission. Choline 50-57 solute carrier family 5 member 7 Homo sapiens 9-12 25970623-10 2015 High-affinity choline transporter (CHT) takes choline into cholinergic neurons for acetylcholine synthesis. Choline 14-21 solute carrier family 5 member 7 Homo sapiens 35-38 25970623-10 2015 High-affinity choline transporter (CHT) takes choline into cholinergic neurons for acetylcholine synthesis. Acetylcholine 83-96 solute carrier family 5 member 7 Homo sapiens 14-33 25970623-10 2015 High-affinity choline transporter (CHT) takes choline into cholinergic neurons for acetylcholine synthesis. Acetylcholine 83-96 solute carrier family 5 member 7 Homo sapiens 35-38 26161852-3 2015 Synthesis and release of the neurotransmitter acetylcholine (ACh) is closely linked to the activity of the high-affinity choline transporter protein (CHT), but the impact of insulin receptor signaling and neuronal insulin resistance on these aspects of cholinergic function are unknown. Acetylcholine 46-59 solute carrier family 5 member 7 Homo sapiens 150-153 26161852-3 2015 Synthesis and release of the neurotransmitter acetylcholine (ACh) is closely linked to the activity of the high-affinity choline transporter protein (CHT), but the impact of insulin receptor signaling and neuronal insulin resistance on these aspects of cholinergic function are unknown. Acetylcholine 61-64 solute carrier family 5 member 7 Homo sapiens 150-153 25536497-4 2015 Specifically, we scanned (BOLD fMRI) participants with a polymorphism of the choline transporter gene that is thought to limit choline transport capacity (Ile89Val variant of the choline transporter gene SLC5A7, rs1013940) and matched controls while they completed a task previously used to demonstrate demand-related increases in right PFC cholinergic transmission in rats and right PFC activation in humans. Choline 77-84 solute carrier family 5 member 7 Homo sapiens 204-210 25073461-5 2014 A single nucleotide polymorphism (SNP) for human CHT1 has been identified, which has a replacement from isoleucine to valine in the third transmembrane segment and shows the choline uptake activity of 50-60% as much as that of wild-type CHT1. Isoleucine 104-114 solute carrier family 5 member 7 Homo sapiens 237-241 25073461-5 2014 A single nucleotide polymorphism (SNP) for human CHT1 has been identified, which has a replacement from isoleucine to valine in the third transmembrane segment and shows the choline uptake activity of 50-60% as much as that of wild-type CHT1. Valine 118-124 solute carrier family 5 member 7 Homo sapiens 49-53 25073461-5 2014 A single nucleotide polymorphism (SNP) for human CHT1 has been identified, which has a replacement from isoleucine to valine in the third transmembrane segment and shows the choline uptake activity of 50-60% as much as that of wild-type CHT1. Valine 118-124 solute carrier family 5 member 7 Homo sapiens 237-241 25073461-5 2014 A single nucleotide polymorphism (SNP) for human CHT1 has been identified, which has a replacement from isoleucine to valine in the third transmembrane segment and shows the choline uptake activity of 50-60% as much as that of wild-type CHT1. Choline 174-181 solute carrier family 5 member 7 Homo sapiens 49-53 25073461-5 2014 A single nucleotide polymorphism (SNP) for human CHT1 has been identified, which has a replacement from isoleucine to valine in the third transmembrane segment and shows the choline uptake activity of 50-60% as much as that of wild-type CHT1. Choline 174-181 solute carrier family 5 member 7 Homo sapiens 237-241 25073461-2 2014 CHT1 is present on the presynaptic terminal of cholinergic neurons, and takes up choline which is the precursor of acetylcholine. Acetylcholine 115-128 solute carrier family 5 member 7 Homo sapiens 0-4 25073461-3 2014 The Na(+)-dependent uptake of choline by CHT1 is the rate-limiting step for synthesis of acetylcholine. Choline 30-37 solute carrier family 5 member 7 Homo sapiens 41-45 25073461-3 2014 The Na(+)-dependent uptake of choline by CHT1 is the rate-limiting step for synthesis of acetylcholine. Acetylcholine 89-102 solute carrier family 5 member 7 Homo sapiens 41-45 25073461-5 2014 A single nucleotide polymorphism (SNP) for human CHT1 has been identified, which has a replacement from isoleucine to valine in the third transmembrane segment and shows the choline uptake activity of 50-60% as much as that of wild-type CHT1. Isoleucine 104-114 solute carrier family 5 member 7 Homo sapiens 49-53 24127780-1 2014 The sodium-coupled, hemicholinium-3-sensitive, high-affinity choline transporter (CHT) is responsible for transport of choline into cholinergic nerve terminals from the synaptic cleft following acetylcholine release and hydrolysis. Choline 61-68 solute carrier family 5 member 7 Homo sapiens 82-85 24525247-5 2014 In this study, using a cholinergic neuronal cell line, SN56, as an invitro model, we detected the physical interaction of UCHL1 and high affinity choline transporter (CHT), which is a key protein regulating Ach re-synthesis. Acetylcholine 207-210 solute carrier family 5 member 7 Homo sapiens 146-165 24525247-5 2014 In this study, using a cholinergic neuronal cell line, SN56, as an invitro model, we detected the physical interaction of UCHL1 and high affinity choline transporter (CHT), which is a key protein regulating Ach re-synthesis. Acetylcholine 207-210 solute carrier family 5 member 7 Homo sapiens 167-170 24127780-1 2014 The sodium-coupled, hemicholinium-3-sensitive, high-affinity choline transporter (CHT) is responsible for transport of choline into cholinergic nerve terminals from the synaptic cleft following acetylcholine release and hydrolysis. Acetylcholine 194-207 solute carrier family 5 member 7 Homo sapiens 4-80 24127780-1 2014 The sodium-coupled, hemicholinium-3-sensitive, high-affinity choline transporter (CHT) is responsible for transport of choline into cholinergic nerve terminals from the synaptic cleft following acetylcholine release and hydrolysis. Acetylcholine 194-207 solute carrier family 5 member 7 Homo sapiens 82-85 24127780-2 2014 In this study, we address regulation of CHT function by plasma membrane cholesterol. Cholesterol 72-83 solute carrier family 5 member 7 Homo sapiens 40-43 24127780-3 2014 We show for the first time that CHT is concentrated in cholesterol-rich lipid rafts in both SH-SY5Y cells and nerve terminals from mouse forebrain. Cholesterol 55-66 solute carrier family 5 member 7 Homo sapiens 32-35 24127780-5 2014 In contrast, CHT activity was increased by addition of cholesterol to membranes using cholesterol-saturated MbetaC. Cholesterol 55-66 solute carrier family 5 member 7 Homo sapiens 13-16 24127780-5 2014 In contrast, CHT activity was increased by addition of cholesterol to membranes using cholesterol-saturated MbetaC. Cholesterol 86-97 solute carrier family 5 member 7 Homo sapiens 13-16 24127780-6 2014 Kinetic analysis of binding of [(3)H]hemicholinium-3 to CHT revealed that reducing membrane cholesterol with MbetaC decreased both the apparent binding affinity (KD) and maximum number of binding sites (Bmax ); this was confirmed by decreased plasma membrane CHT protein in lipid rafts in cell surface protein biotinylation assays. Cholesterol 92-103 solute carrier family 5 member 7 Homo sapiens 56-59 24127780-6 2014 Kinetic analysis of binding of [(3)H]hemicholinium-3 to CHT revealed that reducing membrane cholesterol with MbetaC decreased both the apparent binding affinity (KD) and maximum number of binding sites (Bmax ); this was confirmed by decreased plasma membrane CHT protein in lipid rafts in cell surface protein biotinylation assays. Cholesterol 92-103 solute carrier family 5 member 7 Homo sapiens 259-262 24127780-8 2014 In summary, we provide evidence that CHT association with cholesterol-rich rafts is critical for transporter function and localization. Cholesterol 58-69 solute carrier family 5 member 7 Homo sapiens 37-40 24127780-10 2014 The sodium-coupled choline transporter CHT moves choline into cholinergic nerve terminals to serve as substrate for acetylcholine synthesis. Choline 19-26 solute carrier family 5 member 7 Homo sapiens 39-42 24127780-10 2014 The sodium-coupled choline transporter CHT moves choline into cholinergic nerve terminals to serve as substrate for acetylcholine synthesis. Acetylcholine 116-129 solute carrier family 5 member 7 Homo sapiens 39-42 24127780-11 2014 We show for the first time that CHT is concentrated in cholesterol-rich lipid rafts, and decreasing membrane cholesterol significantly reduces both choline uptake activity and cell surface CHT protein levels. Cholesterol 55-66 solute carrier family 5 member 7 Homo sapiens 32-35 24127780-11 2014 We show for the first time that CHT is concentrated in cholesterol-rich lipid rafts, and decreasing membrane cholesterol significantly reduces both choline uptake activity and cell surface CHT protein levels. Cholesterol 109-120 solute carrier family 5 member 7 Homo sapiens 32-35 24127780-11 2014 We show for the first time that CHT is concentrated in cholesterol-rich lipid rafts, and decreasing membrane cholesterol significantly reduces both choline uptake activity and cell surface CHT protein levels. Cholesterol 109-120 solute carrier family 5 member 7 Homo sapiens 189-192 24127780-11 2014 We show for the first time that CHT is concentrated in cholesterol-rich lipid rafts, and decreasing membrane cholesterol significantly reduces both choline uptake activity and cell surface CHT protein levels. Choline 148-155 solute carrier family 5 member 7 Homo sapiens 32-35 24127780-12 2014 CHT association with cholesterol-rich rafts is critical for its function, and alterations in plasma membrane cholesterol could diminish cholinergic transmission by reducing choline availability for acetylcholine synthesis. Cholesterol 21-32 solute carrier family 5 member 7 Homo sapiens 0-3 24127780-12 2014 CHT association with cholesterol-rich rafts is critical for its function, and alterations in plasma membrane cholesterol could diminish cholinergic transmission by reducing choline availability for acetylcholine synthesis. Acetylcholine 198-211 solute carrier family 5 member 7 Homo sapiens 0-3 23077721-6 2012 We established that the addition of choline to these cells, at concentrations appropriate for high-affinity choline transport at presynaptic terminals, generates a hemicholinium-3 (HC-3)-sensitive, membrane depolarization that can be used for the screening of CHT inhibitors and activators. Choline 36-43 solute carrier family 5 member 7 Homo sapiens 260-263 23651124-2 2013 In neurons, the Na(+) -dependent, high affinity choline transporter (CHT1) is absolutely required for ACh synthesis. Acetylcholine 102-105 solute carrier family 5 member 7 Homo sapiens 69-73 23651124-4 2013 The aim of this study was to identify choline transporters, other than CHT1, that play a role in non-neuronal ACh synthesis. Acetylcholine 110-113 solute carrier family 5 member 7 Homo sapiens 71-75 23399274-1 2013 Novel poly(vinyl alcohol)/citric acid/chitosan (PVA/CA/CHT, PCC) beads were prepared as an adsorbent for the removal of trivalent chromium (Cr(3+)) in aqueous solutions. Polyvinyl Alcohol 6-25 solute carrier family 5 member 7 Homo sapiens 55-58 23132865-1 2012 The high-affinity choline transporter CHT1 mediates choline uptake essential for acetylcholine synthesis in cholinergic nerve terminals. Choline 18-25 solute carrier family 5 member 7 Homo sapiens 38-42 23132865-1 2012 The high-affinity choline transporter CHT1 mediates choline uptake essential for acetylcholine synthesis in cholinergic nerve terminals. Acetylcholine 81-94 solute carrier family 5 member 7 Homo sapiens 38-42 23132865-3 2012 We examined the transmembrane topology of human CHT1 using cysteine-scanning analysis. Cysteine 59-67 solute carrier family 5 member 7 Homo sapiens 48-52 22828453-10 2012 Comparing BL with ChT1, BL with ChT3, and ChT1 with ChT3, the CV of the liver SUL MEAN was 10.4, 10.7, and 10.3%; nevertheless, in 34.0, 38.3, and 36.6% of these examinations, respectively, the liver average SUL MEAN values exceeded the limit for inclusion in the study; that is, the difference was less than +- 0.3 U and +- 20%. Sulfisoxazole 78-81 solute carrier family 5 member 7 Homo sapiens 42-46 24261905-10 2013 CONCLUSION: In this preliminary study of few patients, we have observed a potential usefulness of Supportan( ) in the compliance of concurrent RT-CHT in patients with H&N cancers; moreover, its administration was useful to maintain the initial biochemical nutritional profile. Adenosine Monophosphate 169-172 solute carrier family 5 member 7 Homo sapiens 146-149 24036453-2 2013 We have mapped preferred productive binding modes of chito-oligosaccharide substrates to HCHT and the data show that HCHT has strong binding affinity in the +3 subsite. oligochitosan 53-74 solute carrier family 5 member 7 Homo sapiens 89-93 24036453-2 2013 We have mapped preferred productive binding modes of chito-oligosaccharide substrates to HCHT and the data show that HCHT has strong binding affinity in the +3 subsite. oligochitosan 53-74 solute carrier family 5 member 7 Homo sapiens 117-121 23359170-4 2013 RT-CHT was superior to RT alone in terms of both OS (MST 19 vs. 12 months, respectively, 5-year OS 18 vs. 6 %, respectively; p = 0.003) and LPFS (MTLP 21 vs. 15 months, respectively, 5-year LPFS 28 vs. 0 %; p = 0.06), but not the DMFS (p = 0.43). dmfs 230-234 solute carrier family 5 member 7 Homo sapiens 3-6 23399274-1 2013 Novel poly(vinyl alcohol)/citric acid/chitosan (PVA/CA/CHT, PCC) beads were prepared as an adsorbent for the removal of trivalent chromium (Cr(3+)) in aqueous solutions. Chitosan 38-46 solute carrier family 5 member 7 Homo sapiens 55-58 23399274-1 2013 Novel poly(vinyl alcohol)/citric acid/chitosan (PVA/CA/CHT, PCC) beads were prepared as an adsorbent for the removal of trivalent chromium (Cr(3+)) in aqueous solutions. Chromium 130-138 solute carrier family 5 member 7 Homo sapiens 55-58 23013069-1 2013 BACKGROUND: A major problem in the treatment of patients with congenital hypothyroidism (CHT) is that the optimal individual target values of thyrotropin (TSH) and free thyroxine (fT(4)) are unknown. Thyroxine 169-178 solute carrier family 5 member 7 Homo sapiens 89-92 23141292-2 2012 Using linkage analysis and whole-exome sequencing of DNA samples from subjects with distal hereditary motor neuropathy type VII, we identified a mutation in SLC5A7, which encodes the presynaptic choline transporter (CHT), a critical determinant of synaptic acetylcholine synthesis and release at the NMJ. Acetylcholine 257-270 solute carrier family 5 member 7 Homo sapiens 157-163 23141292-2 2012 Using linkage analysis and whole-exome sequencing of DNA samples from subjects with distal hereditary motor neuropathy type VII, we identified a mutation in SLC5A7, which encodes the presynaptic choline transporter (CHT), a critical determinant of synaptic acetylcholine synthesis and release at the NMJ. Acetylcholine 257-270 solute carrier family 5 member 7 Homo sapiens 195-214 23141292-2 2012 Using linkage analysis and whole-exome sequencing of DNA samples from subjects with distal hereditary motor neuropathy type VII, we identified a mutation in SLC5A7, which encodes the presynaptic choline transporter (CHT), a critical determinant of synaptic acetylcholine synthesis and release at the NMJ. Acetylcholine 257-270 solute carrier family 5 member 7 Homo sapiens 216-219 23077721-6 2012 We established that the addition of choline to these cells, at concentrations appropriate for high-affinity choline transport at presynaptic terminals, generates a hemicholinium-3 (HC-3)-sensitive, membrane depolarization that can be used for the screening of CHT inhibitors and activators. Choline 108-115 solute carrier family 5 member 7 Homo sapiens 260-263 23077721-6 2012 We established that the addition of choline to these cells, at concentrations appropriate for high-affinity choline transport at presynaptic terminals, generates a hemicholinium-3 (HC-3)-sensitive, membrane depolarization that can be used for the screening of CHT inhibitors and activators. Hemicholinium 3 164-179 solute carrier family 5 member 7 Homo sapiens 260-263 23077721-6 2012 We established that the addition of choline to these cells, at concentrations appropriate for high-affinity choline transport at presynaptic terminals, generates a hemicholinium-3 (HC-3)-sensitive, membrane depolarization that can be used for the screening of CHT inhibitors and activators. Hemicholinium 3 181-186 solute carrier family 5 member 7 Homo sapiens 260-263 23077721-7 2012 Using this assay, we discovered that staurosporine increased CHT LV-AA choline uptake activity, an effect mediated by a decrease in choline K(M) with no change in V(max). Staurosporine 37-50 solute carrier family 5 member 7 Homo sapiens 61-64 23077721-7 2012 Using this assay, we discovered that staurosporine increased CHT LV-AA choline uptake activity, an effect mediated by a decrease in choline K(M) with no change in V(max). Choline 71-78 solute carrier family 5 member 7 Homo sapiens 61-64 23077721-7 2012 Using this assay, we discovered that staurosporine increased CHT LV-AA choline uptake activity, an effect mediated by a decrease in choline K(M) with no change in V(max). Choline 132-139 solute carrier family 5 member 7 Homo sapiens 61-64 23077721-9 2012 Surprisingly, staurosporine reduced choline-induced membrane depolarization, suggesting that increased substrate coupling to ion gradients, arising at the expense of nonstoichiometric ion flow, accompanies a shift of CHT to a higher-affinity state. Staurosporine 14-27 solute carrier family 5 member 7 Homo sapiens 217-220 23077721-9 2012 Surprisingly, staurosporine reduced choline-induced membrane depolarization, suggesting that increased substrate coupling to ion gradients, arising at the expense of nonstoichiometric ion flow, accompanies a shift of CHT to a higher-affinity state. Choline 36-43 solute carrier family 5 member 7 Homo sapiens 217-220 22483273-1 2012 Choline uptake into cholinergic nerve terminals by the sodium-dependent high-affinity choline transporter CHT is essential for providing choline as substrate for synthesis of acetylcholine (ACh); ACh is used by cholinergic neurons to communicate information to a wide range of tissues in central and peripheral nervous systems. Choline 0-7 solute carrier family 5 member 7 Homo sapiens 106-109 22483273-1 2012 Choline uptake into cholinergic nerve terminals by the sodium-dependent high-affinity choline transporter CHT is essential for providing choline as substrate for synthesis of acetylcholine (ACh); ACh is used by cholinergic neurons to communicate information to a wide range of tissues in central and peripheral nervous systems. Sodium 55-61 solute carrier family 5 member 7 Homo sapiens 106-109 22483273-1 2012 Choline uptake into cholinergic nerve terminals by the sodium-dependent high-affinity choline transporter CHT is essential for providing choline as substrate for synthesis of acetylcholine (ACh); ACh is used by cholinergic neurons to communicate information to a wide range of tissues in central and peripheral nervous systems. Choline 20-27 solute carrier family 5 member 7 Homo sapiens 106-109 22483273-1 2012 Choline uptake into cholinergic nerve terminals by the sodium-dependent high-affinity choline transporter CHT is essential for providing choline as substrate for synthesis of acetylcholine (ACh); ACh is used by cholinergic neurons to communicate information to a wide range of tissues in central and peripheral nervous systems. Acetylcholine 175-188 solute carrier family 5 member 7 Homo sapiens 106-109 22483273-1 2012 Choline uptake into cholinergic nerve terminals by the sodium-dependent high-affinity choline transporter CHT is essential for providing choline as substrate for synthesis of acetylcholine (ACh); ACh is used by cholinergic neurons to communicate information to a wide range of tissues in central and peripheral nervous systems. Acetylcholine 190-193 solute carrier family 5 member 7 Homo sapiens 106-109 22483273-1 2012 Choline uptake into cholinergic nerve terminals by the sodium-dependent high-affinity choline transporter CHT is essential for providing choline as substrate for synthesis of acetylcholine (ACh); ACh is used by cholinergic neurons to communicate information to a wide range of tissues in central and peripheral nervous systems. Acetylcholine 196-199 solute carrier family 5 member 7 Homo sapiens 106-109 22483273-4 2012 Thus, choline uptake activity is determined largely by the plasma membrane CHT level, and this is finely controlled by a balance between internalization and recycling of CHT proteins in endosomal compartments. Choline 6-13 solute carrier family 5 member 7 Homo sapiens 75-78 22483273-4 2012 Thus, choline uptake activity is determined largely by the plasma membrane CHT level, and this is finely controlled by a balance between internalization and recycling of CHT proteins in endosomal compartments. Choline 6-13 solute carrier family 5 member 7 Homo sapiens 170-173 22483273-5 2012 CHT proteins are also in synaptic vesicle membranes, thereby allowing cell surface CHT levels to increase rapidly in conjunction with exocytotic transmitter release to provide enhanced choline for ACh re-synthesis. Choline 185-192 solute carrier family 5 member 7 Homo sapiens 0-3 22483273-5 2012 CHT proteins are also in synaptic vesicle membranes, thereby allowing cell surface CHT levels to increase rapidly in conjunction with exocytotic transmitter release to provide enhanced choline for ACh re-synthesis. Choline 185-192 solute carrier family 5 member 7 Homo sapiens 83-86 22483273-5 2012 CHT proteins are also in synaptic vesicle membranes, thereby allowing cell surface CHT levels to increase rapidly in conjunction with exocytotic transmitter release to provide enhanced choline for ACh re-synthesis. Acetylcholine 197-200 solute carrier family 5 member 7 Homo sapiens 0-3 22483273-5 2012 CHT proteins are also in synaptic vesicle membranes, thereby allowing cell surface CHT levels to increase rapidly in conjunction with exocytotic transmitter release to provide enhanced choline for ACh re-synthesis. Acetylcholine 197-200 solute carrier family 5 member 7 Homo sapiens 83-86 22483273-9 2012 The critical role of CHT in maintaining cholinergic transmission indicates that it could be a target for therapeutic intervention to promote ACh synthesis, but mechanisms by which this can be accomplished have not been adequately addressed. Acetylcholine 141-144 solute carrier family 5 member 7 Homo sapiens 21-24 22514319-8 2012 Lysosome inhibitors increase choline uptake, suggesting that CHT proteins are normally degraded by lysosomes, and this is not altered by oxidative stress. Choline 29-36 solute carrier family 5 member 7 Homo sapiens 61-64 22514319-9 2012 Unexpectedly, inhibitors of proteasomes, but not lysosomes, attenuate SIN-1-mediated inhibition of choline uptake, indicating that proteasomal degradation plays a role in regulating CHT disposition in SIN-1-treated cells. Choline 99-106 solute carrier family 5 member 7 Homo sapiens 182-185 22192075-3 2012 We have studied the properties of HCHT by analyzing how the enzyme acts on high-molecular weight chitosans, soluble copolymers of beta-1,4-linked N-acetylglucosamine (GlcNAc, A), and glucosamine (GlcN, D). copolymers 116-126 solute carrier family 5 member 7 Homo sapiens 34-38 22361880-1 2012 The high-affinity choline transporter (CHT1), which is specifically expressed in cholinergic neurons, constitutes a rate-limiting step for acetylcholine synthesis. Acetylcholine 139-152 solute carrier family 5 member 7 Homo sapiens 39-43 22361880-2 2012 We have found that the exogenous ubiquitin ligase Nedd4-2 interacts with CHT1 expressed in HEK293 cells decreasing the amount of cell surface CHT1 by approximately 40%, and that small interfering RNA for endogenous Nedd4-2 enhances the choline uptake activity by CHT1 in HEK293 cells. Choline 236-243 solute carrier family 5 member 7 Homo sapiens 73-77 22361880-3 2012 These results indicate that Nedd4-2-mediated ubiquitination regulates the cell surface expression of CHT1 in cultured cells and suggest a possibility that treatments or drugs which inhibit the interaction between CHT1 and Nedd4-2 might be useful for diseases involving decrease in acetylcholine level such as Alzheimer"s disease. Acetylcholine 281-294 solute carrier family 5 member 7 Homo sapiens 101-105 22361880-3 2012 These results indicate that Nedd4-2-mediated ubiquitination regulates the cell surface expression of CHT1 in cultured cells and suggest a possibility that treatments or drugs which inhibit the interaction between CHT1 and Nedd4-2 might be useful for diseases involving decrease in acetylcholine level such as Alzheimer"s disease. Acetylcholine 281-294 solute carrier family 5 member 7 Homo sapiens 213-217 22192075-3 2012 We have studied the properties of HCHT by analyzing how the enzyme acts on high-molecular weight chitosans, soluble copolymers of beta-1,4-linked N-acetylglucosamine (GlcNAc, A), and glucosamine (GlcN, D). beta-1,4-linked n-acetylglucosamine 130-165 solute carrier family 5 member 7 Homo sapiens 34-38 22192075-3 2012 We have studied the properties of HCHT by analyzing how the enzyme acts on high-molecular weight chitosans, soluble copolymers of beta-1,4-linked N-acetylglucosamine (GlcNAc, A), and glucosamine (GlcN, D). 2-acetamido-2-deoxy-4-O-(beta-2-acetamid-2-deoxyglucopyranosyl)glucopyranose 167-173 solute carrier family 5 member 7 Homo sapiens 34-38 22192075-3 2012 We have studied the properties of HCHT by analyzing how the enzyme acts on high-molecular weight chitosans, soluble copolymers of beta-1,4-linked N-acetylglucosamine (GlcNAc, A), and glucosamine (GlcN, D). Glucosamine 154-165 solute carrier family 5 member 7 Homo sapiens 34-38 22192075-3 2012 We have studied the properties of HCHT by analyzing how the enzyme acts on high-molecular weight chitosans, soluble copolymers of beta-1,4-linked N-acetylglucosamine (GlcNAc, A), and glucosamine (GlcN, D). Glucosamine 167-171 solute carrier family 5 member 7 Homo sapiens 34-38 22192075-5 2012 The preferences of HCHT subsites for acetylated versus nonacetylated sugars were assessed by sequence analysis of obtained oligomeric products showing a very strong, absolute, and a relative weak preference for an acetylated unit in the -2, -1, and +1 subsites, respectively. Sugars 69-75 solute carrier family 5 member 7 Homo sapiens 19-23 21898555-1 2011 The influence of coexisting humic acids (HA) or Cu2+ on the photodegradation of pesticides lambda-cyhalothrin (lambda-CHT) and cypermethrin (CPM) in aqueous solution was studied under xenon lamp irradiation. cyhalothrin 98-109 solute carrier family 5 member 7 Homo sapiens 118-121 21644562-6 2011 We critically analyze results of materials development in the areas most impacted by the CHT approaches, such as catalysis, electronic and functional materials, polymer-based industrial coatings, sensing materials, and biomaterials. Polymers 161-168 solute carrier family 5 member 7 Homo sapiens 89-92 22107254-1 2011 Addition of LiOHMT (OHMT = O-2,6-dimesitylphenoxide) to W(O)(CH-t-Bu)(PMe(2)Ph)(2)Cl(2) led to WO(CH-t-Bu)Cl(OHMT)(PMe(2)Ph) (4). liohmt 12-18 solute carrier family 5 member 7 Homo sapiens 61-65 22107254-1 2011 Addition of LiOHMT (OHMT = O-2,6-dimesitylphenoxide) to W(O)(CH-t-Bu)(PMe(2)Ph)(2)Cl(2) led to WO(CH-t-Bu)Cl(OHMT)(PMe(2)Ph) (4). liohmt 12-18 solute carrier family 5 member 7 Homo sapiens 98-102 22107254-1 2011 Addition of LiOHMT (OHMT = O-2,6-dimesitylphenoxide) to W(O)(CH-t-Bu)(PMe(2)Ph)(2)Cl(2) led to WO(CH-t-Bu)Cl(OHMT)(PMe(2)Ph) (4). o-2,6-dimesitylphenoxide 27-51 solute carrier family 5 member 7 Homo sapiens 61-65 22107254-1 2011 Addition of LiOHMT (OHMT = O-2,6-dimesitylphenoxide) to W(O)(CH-t-Bu)(PMe(2)Ph)(2)Cl(2) led to WO(CH-t-Bu)Cl(OHMT)(PMe(2)Ph) (4). o-2,6-dimesitylphenoxide 27-51 solute carrier family 5 member 7 Homo sapiens 98-102 22107254-2 2011 Subsequent addition of Li(2,5-Me(2)C(4)H(2)N) to 4 yielded yellow W(O)(CH-t-Bu)(OHMT)(Me(2)Pyr)(PMe(2)Ph) (5). li(2,5-me 23-32 solute carrier family 5 member 7 Homo sapiens 71-75 22107254-2 2011 Subsequent addition of Li(2,5-Me(2)C(4)H(2)N) to 4 yielded yellow W(O)(CH-t-Bu)(OHMT)(Me(2)Pyr)(PMe(2)Ph) (5). Nitrogen 43-44 solute carrier family 5 member 7 Homo sapiens 71-75 22107254-2 2011 Subsequent addition of Li(2,5-Me(2)C(4)H(2)N) to 4 yielded yellow W(O)(CH-t-Bu)(OHMT)(Me(2)Pyr)(PMe(2)Ph) (5). w(o) 66-70 solute carrier family 5 member 7 Homo sapiens 71-75 21898555-2 2011 The removal efficiency of pesticides lambda-CHT and CPM were enhanced in the presence of either Cu2+ or HA but restrained in the presence of both Cu2+ and HA. cupric ion 96-100 solute carrier family 5 member 7 Homo sapiens 44-47 21898555-2 2011 The removal efficiency of pesticides lambda-CHT and CPM were enhanced in the presence of either Cu2+ or HA but restrained in the presence of both Cu2+ and HA. cupric ion 146-150 solute carrier family 5 member 7 Homo sapiens 44-47 20053562-5 2010 These models will allow the rapid in silico screening of binding affinity at the BBB CHT of both known nicotinic receptor antagonists and virtual compound libraries with the goal of informing the design of brain bioavailable quaternary ammonium analogs that are high affinity selective nicotinic receptor antagonists. quaternary ammonium 225-244 solute carrier family 5 member 7 Homo sapiens 85-88 20637607-1 2010 The high affinity neuronal choline transporter (CHT1) is responsible for the uptake of choline into the pre-synaptic terminal of cholinergic neurons. Choline 27-34 solute carrier family 5 member 7 Homo sapiens 48-52 20490865-3 2010 Choline reuptake at the synapse occurs via the high-affinity choline transporter (CHT1). Choline 0-7 solute carrier family 5 member 7 Homo sapiens 82-86 22016532-2 2011 The high-affinity choline transporter CHT1 is responsible for choline uptake, the rate-limiting step in acetylcholine synthesis. Choline 18-25 solute carrier family 5 member 7 Homo sapiens 38-42 22016532-2 2011 The high-affinity choline transporter CHT1 is responsible for choline uptake, the rate-limiting step in acetylcholine synthesis. Acetylcholine 104-117 solute carrier family 5 member 7 Homo sapiens 38-42 22016532-3 2011 However, endogenous physiological factors that affect CHT1 expression or function and consequently regulate the acetylcholine synthesis rate are essentially unknown. Acetylcholine 112-125 solute carrier family 5 member 7 Homo sapiens 54-58 22016532-5 2011 Extracellular choline rapidly decreases cell-surface CHT1 expression by accelerating its internalization, a process that is mediated by a dynamin-dependent endocytosis pathway in HEK293 cells. Choline 14-21 solute carrier family 5 member 7 Homo sapiens 53-57 22016532-6 2011 Specific inhibitor hemicholinium-3 decreases the constitutive internalization rate and thereby increases cell-surface CHT1 expression. Hemicholinium 3 19-34 solute carrier family 5 member 7 Homo sapiens 118-122 22016532-8 2011 Our results collectively suggest that the internalization of CHT1 is induced by extracellular substrate, providing a novel feedback mechanism for the regulation of acetylcholine synthesis at the cholinergic presynaptic terminals. Acetylcholine 164-177 solute carrier family 5 member 7 Homo sapiens 61-65 17386527-3 2007 The addition of CHT increases the fluorescence of BC but decreases the fluorescence of PC, and glucose addition gives in both cases no spectral changes. pc 87-89 solute carrier family 5 member 7 Homo sapiens 16-19 18088276-1 2007 The high-affinity choline transporter (CHT1) is responsible for uptake of choline from the synaptic cleft and supplying choline for acetylcholine synthesis. Choline 18-25 solute carrier family 5 member 7 Homo sapiens 39-43 18088276-1 2007 The high-affinity choline transporter (CHT1) is responsible for uptake of choline from the synaptic cleft and supplying choline for acetylcholine synthesis. Choline 74-81 solute carrier family 5 member 7 Homo sapiens 39-43 18088276-1 2007 The high-affinity choline transporter (CHT1) is responsible for uptake of choline from the synaptic cleft and supplying choline for acetylcholine synthesis. Acetylcholine 132-145 solute carrier family 5 member 7 Homo sapiens 39-43 18088276-2 2007 CHT1 internalization by clathrin-coated vesicles is proposed to represent a mechanism by which high-affinity choline uptake can be modulated. Choline 109-116 solute carrier family 5 member 7 Homo sapiens 0-4 17196556-8 2007 Since human neocortex has recently been shown to possess intrinsic cholinergic innervation, our results indicate that alterations in CHT1-mediated high affinity choline uptake in cortical neurons may contribute to Alzheimer"s dementia. Choline 67-74 solute carrier family 5 member 7 Homo sapiens 133-137 21547719-3 2009 The presynaptic choline transporter (CHT, SLC5A7) is the major, rate-limiting determinant of ACh production in the brain and periphery and is consequently upregulated during tasks that require sustained attention. Acetylcholine 93-96 solute carrier family 5 member 7 Homo sapiens 16-35 21547719-3 2009 The presynaptic choline transporter (CHT, SLC5A7) is the major, rate-limiting determinant of ACh production in the brain and periphery and is consequently upregulated during tasks that require sustained attention. Acetylcholine 93-96 solute carrier family 5 member 7 Homo sapiens 37-40 21547719-3 2009 The presynaptic choline transporter (CHT, SLC5A7) is the major, rate-limiting determinant of ACh production in the brain and periphery and is consequently upregulated during tasks that require sustained attention. Acetylcholine 93-96 solute carrier family 5 member 7 Homo sapiens 42-48 18581229-3 2009 We investigated the effects of H(2)O(2) and chloramine T(Ch-T) agents known to oxidize both cysteine and methionine residues, and 5, 5"-dithio-bis (2-nitrobenzoic acid) (DTNB)--a cysteine-specific oxidant, on the intracellular calcium in hippocampal neurons. chloramine-T 44-56 solute carrier family 5 member 7 Homo sapiens 57-61 18581229-3 2009 We investigated the effects of H(2)O(2) and chloramine T(Ch-T) agents known to oxidize both cysteine and methionine residues, and 5, 5"-dithio-bis (2-nitrobenzoic acid) (DTNB)--a cysteine-specific oxidant, on the intracellular calcium in hippocampal neurons. Cysteine 92-100 solute carrier family 5 member 7 Homo sapiens 57-61 18581229-3 2009 We investigated the effects of H(2)O(2) and chloramine T(Ch-T) agents known to oxidize both cysteine and methionine residues, and 5, 5"-dithio-bis (2-nitrobenzoic acid) (DTNB)--a cysteine-specific oxidant, on the intracellular calcium in hippocampal neurons. Methionine 105-115 solute carrier family 5 member 7 Homo sapiens 57-61 18581229-5 2009 The elevation induced by H(2)O(2) and Ch-T was significantly higher than DTNB. Dithionitrobenzoic Acid 73-77 solute carrier family 5 member 7 Homo sapiens 38-42 18581229-6 2009 Pretreatment with reductant DTT at 1 mM for 10 min completely prevented the action of DTNB on [Ca(2+)](i), but only partially reduced the effects of H(2)O(2) and Ch-T on [Ca(2+)](i), the reductions were 44.6 +/- 4.2% and 29.6 +/- 6.1% over baseline, respectively. Dithiothreitol 28-31 solute carrier family 5 member 7 Homo sapiens 162-166 18581229-7 2009 The elevation of [Ca(2+)](i) induced by H(2)O(2) and Ch-T after pretreatment with DTT were statistically higher than that induced by single administration of DTNB. Dithiothreitol 82-85 solute carrier family 5 member 7 Homo sapiens 53-57 18581229-7 2009 The elevation of [Ca(2+)](i) induced by H(2)O(2) and Ch-T after pretreatment with DTT were statistically higher than that induced by single administration of DTNB. Dithionitrobenzoic Acid 158-162 solute carrier family 5 member 7 Homo sapiens 53-57 16524384-1 2006 Maintenance of acetylcholine (ACh) synthesis depends on the activity of the high-affinity choline transporter (CHT1), which is responsible for the reuptake of choline from the synaptic cleft into presynaptic neurons. Acetylcholine 15-28 solute carrier family 5 member 7 Homo sapiens 111-115 16636297-8 2006 CHT1 and CTL1 but not OCT transporters are selectively inhibited with hemicholinium-3 and essentially display characteristics of specialized transporters for targeted choline metabolism. Choline 167-174 solute carrier family 5 member 7 Homo sapiens 0-4 16636297-9 2006 CHT1 is abundant in neurons and almost exclusively supplies choline for acetyl-choline synthesis. Choline 60-67 solute carrier family 5 member 7 Homo sapiens 0-4 16636297-9 2006 CHT1 is abundant in neurons and almost exclusively supplies choline for acetyl-choline synthesis. Acetylcholine 72-86 solute carrier family 5 member 7 Homo sapiens 0-4 17005849-1 2006 The recent cloning of the human choline transporter (hCHT) has allowed its expression in Xenopus laevis oocytes and the simultaneous measurement of choline transport and choline-induced current under voltage clamp. Choline 32-39 solute carrier family 5 member 7 Homo sapiens 53-57 17005849-1 2006 The recent cloning of the human choline transporter (hCHT) has allowed its expression in Xenopus laevis oocytes and the simultaneous measurement of choline transport and choline-induced current under voltage clamp. Choline 148-155 solute carrier family 5 member 7 Homo sapiens 53-57 17005849-2 2006 hCHT currents and choline transport are evident in cRNA-injected oocytes and significantly enhanced by the hCHT trafficking mutant L530A/V531A. Choline 18-25 solute carrier family 5 member 7 Homo sapiens 107-111 17005849-3 2006 The charge/choline ratio of hCHT varies from 10e/choline at -80 mV to 3e/choline at -20 mV, in contrast with the reported fixed stoichiometry of the Na+-coupled glucose transporter in the same gene family. Choline 11-18 solute carrier family 5 member 7 Homo sapiens 28-32 17005849-3 2006 The charge/choline ratio of hCHT varies from 10e/choline at -80 mV to 3e/choline at -20 mV, in contrast with the reported fixed stoichiometry of the Na+-coupled glucose transporter in the same gene family. Choline 49-56 solute carrier family 5 member 7 Homo sapiens 28-32 17005849-3 2006 The charge/choline ratio of hCHT varies from 10e/choline at -80 mV to 3e/choline at -20 mV, in contrast with the reported fixed stoichiometry of the Na+-coupled glucose transporter in the same gene family. Choline 49-56 solute carrier family 5 member 7 Homo sapiens 28-32 17005849-5 2006 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline-induced current; in addition, HC-3 alone reveals a constitutive, depolarizing leak current through hCHT. Hemicholinium 3 28-43 solute carrier family 5 member 7 Homo sapiens 4-8 17005849-5 2006 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline-induced current; in addition, HC-3 alone reveals a constitutive, depolarizing leak current through hCHT. Hemicholinium 3 28-43 solute carrier family 5 member 7 Homo sapiens 184-188 17005849-5 2006 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline-induced current; in addition, HC-3 alone reveals a constitutive, depolarizing leak current through hCHT. Hydrocortisone 45-47 solute carrier family 5 member 7 Homo sapiens 4-8 17005849-5 2006 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline-induced current; in addition, HC-3 alone reveals a constitutive, depolarizing leak current through hCHT. Hydrocortisone 45-47 solute carrier family 5 member 7 Homo sapiens 184-188 17005849-5 2006 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline-induced current; in addition, HC-3 alone reveals a constitutive, depolarizing leak current through hCHT. Choline 58-65 solute carrier family 5 member 7 Homo sapiens 4-8 17005849-5 2006 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline-induced current; in addition, HC-3 alone reveals a constitutive, depolarizing leak current through hCHT. Choline 77-84 solute carrier family 5 member 7 Homo sapiens 4-8 17005849-5 2006 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline-induced current; in addition, HC-3 alone reveals a constitutive, depolarizing leak current through hCHT. Hemicholinium 3 45-49 solute carrier family 5 member 7 Homo sapiens 4-8 17005849-5 2006 The hCHT-specific inhibitor hemicholinium-3 (HC-3) blocks choline uptake and choline-induced current; in addition, HC-3 alone reveals a constitutive, depolarizing leak current through hCHT. Hemicholinium 3 45-49 solute carrier family 5 member 7 Homo sapiens 184-188 17005849-6 2006 We show that external protons reduce hCHT current, transport, and binding with a similar pKa of 7.4, suggesting proton titration of residue(s) that support choline binding and transport. Choline 156-163 solute carrier family 5 member 7 Homo sapiens 37-41 17005849-7 2006 Given the localization of the choline transporter to synaptic vesicles, we propose that proton inactivation of hCHT prevents acetylcholine and proton leakage from the acidic interior of cholinergic synaptic vesicles. Acetylcholine 125-138 solute carrier family 5 member 7 Homo sapiens 111-115 16524384-1 2006 Maintenance of acetylcholine (ACh) synthesis depends on the activity of the high-affinity choline transporter (CHT1), which is responsible for the reuptake of choline from the synaptic cleft into presynaptic neurons. Acetylcholine 30-33 solute carrier family 5 member 7 Homo sapiens 111-115 16524384-1 2006 Maintenance of acetylcholine (ACh) synthesis depends on the activity of the high-affinity choline transporter (CHT1), which is responsible for the reuptake of choline from the synaptic cleft into presynaptic neurons. Choline 21-28 solute carrier family 5 member 7 Homo sapiens 111-115 16524384-4 2006 The presence of CHT1 at the plasma membrane is limited by rapid endocytosis of the transporter in clathrin-coated pits in a mechanism dependent on a dileucine-like motif present in the carboxyl-terminal region of the transporter. dileucine 149-158 solute carrier family 5 member 7 Homo sapiens 16-20 16524384-7 2006 Current knowledge about CHT1 indicates that stimulated and constitutive exocytosis, in addition to endocytosis, will have major consequences for regulating choline uptake. Choline 156-163 solute carrier family 5 member 7 Homo sapiens 24-28 17269365-8 2006 After ChT the regression of the disease (PR+CR) was noted in 37% of patients. Chromium 44-46 solute carrier family 5 member 7 Homo sapiens 6-9 16334159-1 2005 BACKGROUND: The impact of radiotherapy alone (RT-alone) and radiotherapy plus concurrent chemotherapy with cisplatin (RT-CHT) was determined for lymphocyte subpopulations in cervical cancer patients. Cisplatin 107-116 solute carrier family 5 member 7 Homo sapiens 121-124 11027560-3 2000 The hCHT cDNA encodes a protein of 580 amino acids having 93% identity to rCHT1 and 51% identity to the Caenorhabditis elegans homolog CHO-1, and is distantly related to members of the Na(+)-coupled glucose transporter (SGLT) gene family of Na(+)-coupled glucose (SGLT), nucleoside and iodide transporters. Glucose 199-206 solute carrier family 5 member 7 Homo sapiens 4-8 15953352-1 2005 Maintenance of acetylcholine synthesis depends on the effective functioning of a high-affinity sodium-dependent choline transporter (CHT1). Acetylcholine 15-28 solute carrier family 5 member 7 Homo sapiens 133-137 15953352-2 2005 Recent studies have shown that this transporter is predominantly localized inside the cell, unlike other neurotransmitter transporters, suggesting that the trafficking of CHT1 to and from the plasma membrane may play a crucial role in regulating choline uptake. Choline 246-253 solute carrier family 5 member 7 Homo sapiens 171-175 15953352-4 2005 CHT1 internalization is controlled by an atypical carboxyl-terminal dileucine-like motif (L531, V532) which, upon replacement by alanine residues, blocks CHT1 internalization in both human embryonic kidney 293 cells and primary cortical neurons and results in both increased CHT1 cell surface expression and choline transport activity. dileucine 68-77 solute carrier family 5 member 7 Homo sapiens 0-4 15953352-4 2005 CHT1 internalization is controlled by an atypical carboxyl-terminal dileucine-like motif (L531, V532) which, upon replacement by alanine residues, blocks CHT1 internalization in both human embryonic kidney 293 cells and primary cortical neurons and results in both increased CHT1 cell surface expression and choline transport activity. dileucine 68-77 solute carrier family 5 member 7 Homo sapiens 154-158 15953352-4 2005 CHT1 internalization is controlled by an atypical carboxyl-terminal dileucine-like motif (L531, V532) which, upon replacement by alanine residues, blocks CHT1 internalization in both human embryonic kidney 293 cells and primary cortical neurons and results in both increased CHT1 cell surface expression and choline transport activity. dileucine 68-77 solute carrier family 5 member 7 Homo sapiens 154-158 15953352-4 2005 CHT1 internalization is controlled by an atypical carboxyl-terminal dileucine-like motif (L531, V532) which, upon replacement by alanine residues, blocks CHT1 internalization in both human embryonic kidney 293 cells and primary cortical neurons and results in both increased CHT1 cell surface expression and choline transport activity. Alanine 129-136 solute carrier family 5 member 7 Homo sapiens 0-4 15953352-4 2005 CHT1 internalization is controlled by an atypical carboxyl-terminal dileucine-like motif (L531, V532) which, upon replacement by alanine residues, blocks CHT1 internalization in both human embryonic kidney 293 cells and primary cortical neurons and results in both increased CHT1 cell surface expression and choline transport activity. Alanine 129-136 solute carrier family 5 member 7 Homo sapiens 154-158 15953352-4 2005 CHT1 internalization is controlled by an atypical carboxyl-terminal dileucine-like motif (L531, V532) which, upon replacement by alanine residues, blocks CHT1 internalization in both human embryonic kidney 293 cells and primary cortical neurons and results in both increased CHT1 cell surface expression and choline transport activity. Alanine 129-136 solute carrier family 5 member 7 Homo sapiens 154-158 15953352-4 2005 CHT1 internalization is controlled by an atypical carboxyl-terminal dileucine-like motif (L531, V532) which, upon replacement by alanine residues, blocks CHT1 internalization in both human embryonic kidney 293 cells and primary cortical neurons and results in both increased CHT1 cell surface expression and choline transport activity. Choline 308-315 solute carrier family 5 member 7 Homo sapiens 0-4 15953352-5 2005 Perturbation of clathrin-mediated endocytosis with dynamin-I K44A increases cell surface expression and transport activity to a similar extent as mutating the dileucine motif, suggesting that we have identified the motif responsible for constitutive CHT1 internalization. dileucine 159-168 solute carrier family 5 member 7 Homo sapiens 250-254 15953352-6 2005 Based on the observation that the localization of CHT1 to the plasma membrane is transient, we propose that acetylcholine synthesis may be influenced by processes that lead to the attenuation of constitutive CHT1 endocytosis. Acetylcholine 108-121 solute carrier family 5 member 7 Homo sapiens 50-54 15635226-12 2005 The 70% release time, T70, of acetaminophen from the matrix particles (Act : Cht=1 : 5) increased in pH 1.2 fluid by about 9-fold and in pH 6.8 fluid by about 5-fold compared to crystalline acetaminophen. Acetaminophen 30-43 solute carrier family 5 member 7 Homo sapiens 77-82 15611726-2 2005 The capacity of the high-affinity choline uptake transporter (CHT) to import choline from the extracellular space to presynaptic terminals is essential for normal acetylcholine synthesis and therefore cholinergic transmission. Choline 34-41 solute carrier family 5 member 7 Homo sapiens 62-65 15611726-2 2005 The capacity of the high-affinity choline uptake transporter (CHT) to import choline from the extracellular space to presynaptic terminals is essential for normal acetylcholine synthesis and therefore cholinergic transmission. Acetylcholine 163-176 solute carrier family 5 member 7 Homo sapiens 62-65 12675135-6 2003 CHT1 mediates Na(+)- and Cl(-)-dependent choline uptake with high sensitivity to hemicholinium-3. Choline 41-48 solute carrier family 5 member 7 Homo sapiens 0-4 12675135-6 2003 CHT1 mediates Na(+)- and Cl(-)-dependent choline uptake with high sensitivity to hemicholinium-3. Hemicholinium 3 81-96 solute carrier family 5 member 7 Homo sapiens 0-4 12628469-7 2003 These results suggest that CHT1 plays a role in mediating choline uptake in T-lymphocytes and provides further evidence for the presence of an independent lymphocytic cholinergic system. Choline 58-65 solute carrier family 5 member 7 Homo sapiens 27-31 11068039-7 2000 The hCHT1-mediated choline uptake increased with increasing concentrations of choline, Na(+) and Cl(-), with EC(50) values of 2.0 microM, 76 mM, and 48 mM, and with apparent Hill coefficients of 1, 2.5 and 2.3, respectively. Choline 19-26 solute carrier family 5 member 7 Homo sapiens 4-9 11068039-7 2000 The hCHT1-mediated choline uptake increased with increasing concentrations of choline, Na(+) and Cl(-), with EC(50) values of 2.0 microM, 76 mM, and 48 mM, and with apparent Hill coefficients of 1, 2.5 and 2.3, respectively. Choline 78-85 solute carrier family 5 member 7 Homo sapiens 4-9 15624938-1 2005 In the present study, we applied for the first time (31)P diffusion NMR to resolve different species obtained by the addition of organophosphorus compounds (OP) such as diisopropyl phosphorofluoridate (DFP) or 1-pyrenebutyl phosphorodichloridate (PBPDC) to alpha-chymotrypsin (Cht). phosphorus-31 atom 52-57 solute carrier family 5 member 7 Homo sapiens 277-280 15624938-1 2005 In the present study, we applied for the first time (31)P diffusion NMR to resolve different species obtained by the addition of organophosphorus compounds (OP) such as diisopropyl phosphorofluoridate (DFP) or 1-pyrenebutyl phosphorodichloridate (PBPDC) to alpha-chymotrypsin (Cht). organophosphorus 129-145 solute carrier family 5 member 7 Homo sapiens 277-280 15624938-6 2005 Diffusion measurements performed on the extensively dialyzed and unfolded DIP-Cht conjugate still resulted in a high diffusion coefficient ((0.30 +/- 0.05) x 10(-5) cm(2) s(-1)) relative to the assumed molecular mass. dip 74-77 solute carrier family 5 member 7 Homo sapiens 78-81 15090548-0 2004 Par-4 inhibits choline uptake by interacting with CHT1 and reducing its incorporation on the plasma membrane. Choline 15-22 solute carrier family 5 member 7 Homo sapiens 50-54 15090548-1 2004 CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline transporter. Hemicholinium 3 38-53 solute carrier family 5 member 7 Homo sapiens 0-4 15090548-1 2004 CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline transporter. Hemicholinium 3 55-60 solute carrier family 5 member 7 Homo sapiens 0-4 15090548-1 2004 CHT1 is a Na(+)- and Cl(-)-dependent, hemicholinium-3 (HC-3)-sensitive, high affinity choline transporter. Choline 86-93 solute carrier family 5 member 7 Homo sapiens 0-4 15090548-3 2004 We now report that Par-4 is a negative regulator of CHT1 choline uptake activity. Choline 57-64 solute carrier family 5 member 7 Homo sapiens 52-56 15090548-4 2004 Transfection of neural IMR-32 cells with human CHT1 conferred Na(+)-dependent, HC-3-sensitive choline uptake that was effectively inhibited by cotransfection of Par-4. Hemicholinium 3 79-83 solute carrier family 5 member 7 Homo sapiens 47-51 15090548-4 2004 Transfection of neural IMR-32 cells with human CHT1 conferred Na(+)-dependent, HC-3-sensitive choline uptake that was effectively inhibited by cotransfection of Par-4. Choline 94-101 solute carrier family 5 member 7 Homo sapiens 47-51 15090548-6 2004 Kinetic and cell-surface biotinylation assays showed that Par-4 inhibited CHT1-mediated choline uptake by reducing CHT1 expression in the plasma membrane without significantly altering the affinity of CHT1 for choline or HC-3. Choline 88-95 solute carrier family 5 member 7 Homo sapiens 74-78 15090548-6 2004 Kinetic and cell-surface biotinylation assays showed that Par-4 inhibited CHT1-mediated choline uptake by reducing CHT1 expression in the plasma membrane without significantly altering the affinity of CHT1 for choline or HC-3. Choline 88-95 solute carrier family 5 member 7 Homo sapiens 115-119 15090548-6 2004 Kinetic and cell-surface biotinylation assays showed that Par-4 inhibited CHT1-mediated choline uptake by reducing CHT1 expression in the plasma membrane without significantly altering the affinity of CHT1 for choline or HC-3. Choline 88-95 solute carrier family 5 member 7 Homo sapiens 115-119 15090548-7 2004 These results suggest that Par-4 is directly involved in regulating choline uptake by interacting with CHT1 and by reducing its incorporation on the cell surface. Choline 68-75 solute carrier family 5 member 7 Homo sapiens 103-107 14993474-1 2004 Presynaptic choline uptake is vital to sustained neuronal acetylcholine (ACh) release; however, only with the recent cloning of choline transporters (CHTs) (i.e., SLC5A7), has a picture emerged of the regulatory pathways supporting CHT modulation. Choline 12-19 solute carrier family 5 member 7 Homo sapiens 163-169 14993474-1 2004 Presynaptic choline uptake is vital to sustained neuronal acetylcholine (ACh) release; however, only with the recent cloning of choline transporters (CHTs) (i.e., SLC5A7), has a picture emerged of the regulatory pathways supporting CHT modulation. Acetylcholine 58-71 solute carrier family 5 member 7 Homo sapiens 163-169 12969261-1 2003 Synthesis of acetylcholine depends on the plasma membrane uptake of choline by a high affinity choline transporter (CHT1). Acetylcholine 13-26 solute carrier family 5 member 7 Homo sapiens 116-120 12969261-1 2003 Synthesis of acetylcholine depends on the plasma membrane uptake of choline by a high affinity choline transporter (CHT1). Choline 19-26 solute carrier family 5 member 7 Homo sapiens 116-120 12969261-2 2003 Choline uptake is regulated by nerve impulses and trafficking of an intracellular pool of CHT1 to the plasma membrane may be important for this regulation. Choline 0-7 solute carrier family 5 member 7 Homo sapiens 90-94 12969261-4 2003 Expression of CHT1-HA in HEK 293 cells establishes Na+-dependent, hemicholinium-3 sensitive high-affinity choline transport activity. Choline 106-113 solute carrier family 5 member 7 Homo sapiens 14-18 12969261-9 2003 We propose that intracellular CHT1 can be recruited during stimulation to increase choline uptake in nerve terminals. Choline 83-90 solute carrier family 5 member 7 Homo sapiens 30-34 12628461-1 2003 Uptake of choline by the high-affinity choline transporter CHT1 is the rate-limiting step in neuronal acetylcholine (ACh) synthesis. Choline 10-17 solute carrier family 5 member 7 Homo sapiens 59-63 12628461-1 2003 Uptake of choline by the high-affinity choline transporter CHT1 is the rate-limiting step in neuronal acetylcholine (ACh) synthesis. Acetylcholine 102-115 solute carrier family 5 member 7 Homo sapiens 59-63 12628461-1 2003 Uptake of choline by the high-affinity choline transporter CHT1 is the rate-limiting step in neuronal acetylcholine (ACh) synthesis. Acetylcholine 117-120 solute carrier family 5 member 7 Homo sapiens 59-63 12628461-6 2003 The close apposition of CHT1 to reported sites of localization of choline acetyltransferase in these cells is strongly in favour of ACh synthesis being fuelled by choline uptake via CHT1 in these epithelia. Acetylcholine 132-135 solute carrier family 5 member 7 Homo sapiens 24-28 12628461-6 2003 The close apposition of CHT1 to reported sites of localization of choline acetyltransferase in these cells is strongly in favour of ACh synthesis being fuelled by choline uptake via CHT1 in these epithelia. Acetylcholine 132-135 solute carrier family 5 member 7 Homo sapiens 182-186 12628461-6 2003 The close apposition of CHT1 to reported sites of localization of choline acetyltransferase in these cells is strongly in favour of ACh synthesis being fuelled by choline uptake via CHT1 in these epithelia. Choline 66-73 solute carrier family 5 member 7 Homo sapiens 24-28 12628469-3 2003 In nerve terminals, choline taken up via the high-affinity choline transporter (CHT1) is exclusively utilized for ACh synthesis. Choline 20-27 solute carrier family 5 member 7 Homo sapiens 80-84 12628469-3 2003 In nerve terminals, choline taken up via the high-affinity choline transporter (CHT1) is exclusively utilized for ACh synthesis. Acetylcholine 114-117 solute carrier family 5 member 7 Homo sapiens 80-84 12628469-6 2003 Consistent with that finding, specific binding of [3H]hemicholinium-3 (HC-3), an inhibitor of CHT1, and HC-3-sensitive [3H]choline uptake were also detected in MOLT-3 cells. Tritium 51-53 solute carrier family 5 member 7 Homo sapiens 94-98 12628469-6 2003 Consistent with that finding, specific binding of [3H]hemicholinium-3 (HC-3), an inhibitor of CHT1, and HC-3-sensitive [3H]choline uptake were also detected in MOLT-3 cells. Hemicholinium 3 54-69 solute carrier family 5 member 7 Homo sapiens 94-98 12628469-6 2003 Consistent with that finding, specific binding of [3H]hemicholinium-3 (HC-3), an inhibitor of CHT1, and HC-3-sensitive [3H]choline uptake were also detected in MOLT-3 cells. Hemicholinium 71-75 solute carrier family 5 member 7 Homo sapiens 94-98 12589972-7 2003 RESULTS: Serum alpha-tocopherol related to the sum of cholesterol and triglyceride concentrations (AT/CHT ratio) was significantly lower in diabetic patients with macroangiopathy than in those without vascular changes (p<0.05). alpha-Tocopherol 15-31 solute carrier family 5 member 7 Homo sapiens 102-105 12939485-4 2003 This ring walk corresponds to interconversion of distonic ions and norcaradiene radical cations (the two intermediates of the TOL/CHT-rearrangement) by making and breaking of the external C-C bonds of the cyclopropane moiety of the intermediate norcaradiene structure. bicyclo(4.1.0)hepta-2,4-diene 67-79 solute carrier family 5 member 7 Homo sapiens 130-133 12939485-4 2003 This ring walk corresponds to interconversion of distonic ions and norcaradiene radical cations (the two intermediates of the TOL/CHT-rearrangement) by making and breaking of the external C-C bonds of the cyclopropane moiety of the intermediate norcaradiene structure. cyclopropane 205-217 solute carrier family 5 member 7 Homo sapiens 130-133 12939485-4 2003 This ring walk corresponds to interconversion of distonic ions and norcaradiene radical cations (the two intermediates of the TOL/CHT-rearrangement) by making and breaking of the external C-C bonds of the cyclopropane moiety of the intermediate norcaradiene structure. bicyclo(4.1.0)hepta-2,4-diene 245-257 solute carrier family 5 member 7 Homo sapiens 130-133 12939485-9 2003 The calculation was repeated for the MERPs of a TOL/CHT-rearrangement of para-xylene radical cation 5 and ethylbenzene radical cation 2, yielding basically the same results as for 1. 4-xylene 73-84 solute carrier family 5 member 7 Homo sapiens 52-55 11027560-5 2000 Expression of hCHT cDNA in COS-7 cells results in saturable, Na(+)/Cl(-)-dependent choline uptake (K(m) = 1.2 microM) in membrane vesicles and [(3)H] HC-3 binding (K(d) = 4 nM) in membrane fractions, consistent with characteristics reported in mammalian cholinergic neurons. carbonyl sulfide 27-30 solute carrier family 5 member 7 Homo sapiens 14-18 11027560-5 2000 Expression of hCHT cDNA in COS-7 cells results in saturable, Na(+)/Cl(-)-dependent choline uptake (K(m) = 1.2 microM) in membrane vesicles and [(3)H] HC-3 binding (K(d) = 4 nM) in membrane fractions, consistent with characteristics reported in mammalian cholinergic neurons. Choline 83-90 solute carrier family 5 member 7 Homo sapiens 14-18 35620456-3 2022 Objective: The present study assessed the cost-effectiveness of lorlatinib vs pemetrexed with platinum combination of carboplatin or cisplatin (P-ChT) in Greece. Cisplatin 133-142 solute carrier family 5 member 7 Homo sapiens 146-149 8740424-3 1996 Interestingly the domain structure of Cht1 is truncated when compared to the other chitinases of C. albicans and lacks a Ser/Thr-rich region. Serine 121-124 solute carrier family 5 member 7 Homo sapiens 38-42 8740424-3 1996 Interestingly the domain structure of Cht1 is truncated when compared to the other chitinases of C. albicans and lacks a Ser/Thr-rich region. Threonine 125-128 solute carrier family 5 member 7 Homo sapiens 38-42 34373737-6 2021 Results: MPC-n(IVIg) efficiently crosses the BBB and IVIg selectively accumulates in ischemic areas in a high-affinity choline transporter 1 (ChT1)-overexpression dependent manner via endothelial cells in ischemic areas. mpc-n 9-14 solute carrier family 5 member 7 Homo sapiens 105-140 34373737-6 2021 Results: MPC-n(IVIg) efficiently crosses the BBB and IVIg selectively accumulates in ischemic areas in a high-affinity choline transporter 1 (ChT1)-overexpression dependent manner via endothelial cells in ischemic areas. mpc-n 9-14 solute carrier family 5 member 7 Homo sapiens 142-146 35232764-0 2022 Disrupted choline clearance and sustained acetylcholine release in vivo by a common choline transporter coding variant associated with poor attentional control in humans. Choline 10-17 solute carrier family 5 member 7 Homo sapiens 84-103 35232764-0 2022 Disrupted choline clearance and sustained acetylcholine release in vivo by a common choline transporter coding variant associated with poor attentional control in humans. Acetylcholine 42-55 solute carrier family 5 member 7 Homo sapiens 84-103 34562520-0 2022 Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein. Choline 0-7 solute carrier family 5 member 7 Homo sapiens 16-22