PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 30613313-4 2018 Changes in the pHi were detected either by microspectrofluorimetry or by a multimode reader with a pH-sensitive fluorescent probe, BCECF, and the fluorescent ratio was calibrated by the high K+/nigericin method. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 131-136 glucose-6-phosphate isomerase Homo sapiens 15-18 30613313-4 2018 Changes in the pHi were detected either by microspectrofluorimetry or by a multimode reader with a pH-sensitive fluorescent probe, BCECF, and the fluorescent ratio was calibrated by the high K+/nigericin method. Nigericin 194-203 glucose-6-phosphate isomerase Homo sapiens 15-18 30464181-1 2018 Clinical benefits of pallidal deep brain stimulation (GPi DBS) in dystonia increase relatively slowly suggesting slow plastic processes in the motor network. dibromsalan 58-61 glucose-6-phosphate isomerase Homo sapiens 54-57 29995453-9 2018 The PGI hits are effective against trypanosomatid PGIs, with IC50 values in the micromolar range, and also against the human homologous enzyme. Prostaglandins I 50-54 glucose-6-phosphate isomerase Homo sapiens 4-7 30464181-4 2018 The acute effects of GPi DBS were associated with a shortening of the motor response whereas the grey matter of chronically treated patients with a better clinical outcome showed hypertrophy of the supplementary motor area and cerebellar vermis. dibromsalan 25-28 glucose-6-phosphate isomerase Homo sapiens 21-24 30464181-6 2018 Importantly, good responders to GPi DBS showed a similar level of short-latency intracortical inhibition in the motor cortex as healthy controls whereas non-responders were unable to increase it. dibromsalan 36-39 glucose-6-phosphate isomerase Homo sapiens 32-35 30033048-4 2018 MATERIALS AND METHODS: In current studies, intracellular pH (pHi) and NHE1 activity were analyzed using the pHi-sensitive dye BCECF-AM. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 126-134 glucose-6-phosphate isomerase Homo sapiens 108-111 30464597-1 2018 Background: Glucose-6-phosphate isomerase (GPI) is a glycolytic-related enzyme that inter-converts glucose-6-phosphate and fructose-6-phosphate in the cytoplasm. Glucose-6-Phosphate 99-118 glucose-6-phosphate isomerase Homo sapiens 12-41 30464597-1 2018 Background: Glucose-6-phosphate isomerase (GPI) is a glycolytic-related enzyme that inter-converts glucose-6-phosphate and fructose-6-phosphate in the cytoplasm. Glucose-6-Phosphate 99-118 glucose-6-phosphate isomerase Homo sapiens 43-46 30464597-1 2018 Background: Glucose-6-phosphate isomerase (GPI) is a glycolytic-related enzyme that inter-converts glucose-6-phosphate and fructose-6-phosphate in the cytoplasm. fructose-6-phosphate 123-143 glucose-6-phosphate isomerase Homo sapiens 12-41 30464597-1 2018 Background: Glucose-6-phosphate isomerase (GPI) is a glycolytic-related enzyme that inter-converts glucose-6-phosphate and fructose-6-phosphate in the cytoplasm. fructose-6-phosphate 123-143 glucose-6-phosphate isomerase Homo sapiens 43-46 30464597-6 2018 Through Kaplan-Meier analysis and according to the Oncomine database, we found that AMF/GPI was overexpressed in GC tissues compared to normal mucosa, and the patients with higher AMF/GPI expression had poorer outcomes. oncomine 51-59 glucose-6-phosphate isomerase Homo sapiens 84-87 30464597-6 2018 Through Kaplan-Meier analysis and according to the Oncomine database, we found that AMF/GPI was overexpressed in GC tissues compared to normal mucosa, and the patients with higher AMF/GPI expression had poorer outcomes. oncomine 51-59 glucose-6-phosphate isomerase Homo sapiens 88-91 30333031-1 2018 BACKGROUND: Sodium/hydrogen exchanger 1 (NHE1), encoded by the SLC9A1 gene (SoLute Carrier family 9A1) in humans, is the main H+ efflux mechanism in maintaining alkaline intracellular pH (pHi) and Warburg effects in glioma. Sodium 12-18 glucose-6-phosphate isomerase Homo sapiens 188-191 30333031-1 2018 BACKGROUND: Sodium/hydrogen exchanger 1 (NHE1), encoded by the SLC9A1 gene (SoLute Carrier family 9A1) in humans, is the main H+ efflux mechanism in maintaining alkaline intracellular pH (pHi) and Warburg effects in glioma. Hydrogen 19-27 glucose-6-phosphate isomerase Homo sapiens 188-191 30478344-7 2018 In turn, GPI maintains glucose metabolism and energy homeostasis in hypoxia by redirecting the glucose flux from androgen/AR-dependent pentose phosphate pathway (PPP) to hypoxia-induced glycolysis pathway, thereby reducing the growth inhibitory effect of enzalutamide. Glucose 23-30 glucose-6-phosphate isomerase Homo sapiens 9-12 30478344-7 2018 In turn, GPI maintains glucose metabolism and energy homeostasis in hypoxia by redirecting the glucose flux from androgen/AR-dependent pentose phosphate pathway (PPP) to hypoxia-induced glycolysis pathway, thereby reducing the growth inhibitory effect of enzalutamide. Pentosephosphates 135-152 glucose-6-phosphate isomerase Homo sapiens 9-12 30478344-7 2018 In turn, GPI maintains glucose metabolism and energy homeostasis in hypoxia by redirecting the glucose flux from androgen/AR-dependent pentose phosphate pathway (PPP) to hypoxia-induced glycolysis pathway, thereby reducing the growth inhibitory effect of enzalutamide. enzalutamide 255-267 glucose-6-phosphate isomerase Homo sapiens 9-12 30478344-8 2018 Inhibiting GPI overcomes the therapy resistance in hypoxia in vitro and increases enzalutamide efficacy in vivo. enzalutamide 82-94 glucose-6-phosphate isomerase Homo sapiens 11-14 30033048-6 2018 As expected, decreased pHi induced by NH4Cl was associated with increased apoptosis, low cell proliferation and ATP depletion, which was exacerbated by exposure to the NHE1 inhibitor cariporide. Ammonium Chloride 38-43 glucose-6-phosphate isomerase Homo sapiens 23-26 30033048-6 2018 As expected, decreased pHi induced by NH4Cl was associated with increased apoptosis, low cell proliferation and ATP depletion, which was exacerbated by exposure to the NHE1 inhibitor cariporide. Adenosine Triphosphate 112-115 glucose-6-phosphate isomerase Homo sapiens 23-26 30033048-6 2018 As expected, decreased pHi induced by NH4Cl was associated with increased apoptosis, low cell proliferation and ATP depletion, which was exacerbated by exposure to the NHE1 inhibitor cariporide. cariporide 183-193 glucose-6-phosphate isomerase Homo sapiens 23-26 30082742-8 2018 In ascidian spermatozoa, the NAC supplementation decreased external pH, which in turn brought to a pHi lowering. Acetylcysteine 29-32 glucose-6-phosphate isomerase Homo sapiens 99-102 29995171-5 2018 For fluorometric recordings, the slice-neurons were loaded with the pHi-sensitive dye BCECF-AM. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 86-94 glucose-6-phosphate isomerase Homo sapiens 68-71 30134708-1 2018 At the limit of stability of a supercooled tetrahedral liquid modeled by monatomic (mW) water potential, it was recently shown that relaxation occurs across a unique value of per particle potential energy (phimid ), which corresponds to a dynamical (non-stationary) condition of Gibbs free energy function G(T, P, N, phi): [ 2(G/N)/ phi2 = 0] and [ (G/N)/ phi 0]. Water 88-93 glucose-6-phosphate isomerase Homo sapiens 206-209 30123518-9 2018 Dystonia did not improve significantly with botulinum toxin, levodopa or trihexyphenidyl, but has shown marked improvement since DBS implantation in the GPi. dibromsalan 129-132 glucose-6-phosphate isomerase Homo sapiens 153-156 29663481-5 2018 Specific inhibition of v-ATPase with bafilomycin and KM91104 induced a down-regulation of the HSC fibrogenic gene profile, which coincided with increased lysosomal pH, decreased pHi, activation of AMPK, reduced proliferation, and lower metabolic activity. bafilomycin 37-48 glucose-6-phosphate isomerase Homo sapiens 178-181 29663481-5 2018 Specific inhibition of v-ATPase with bafilomycin and KM91104 induced a down-regulation of the HSC fibrogenic gene profile, which coincided with increased lysosomal pH, decreased pHi, activation of AMPK, reduced proliferation, and lower metabolic activity. 3,4-dihydroxy-N'-(2-hydroxybenzylidene)benzohydrazide 53-60 glucose-6-phosphate isomerase Homo sapiens 178-181 30094187-6 2018 GPI-APs function in vitamin-B6 and folate transport, nucleotide metabolism and lipid homeostasis. Vitamin B 6 20-30 glucose-6-phosphate isomerase Homo sapiens 0-3 30094187-6 2018 GPI-APs function in vitamin-B6 and folate transport, nucleotide metabolism and lipid homeostasis. Folic Acid 35-41 glucose-6-phosphate isomerase Homo sapiens 0-3 29575508-3 2018 It is also clear that mitogen signalling in nominal absence of HCO3- is associated with an intracellular alkalinization (~0.3 pH unit above steady-state pHi ), which is secondary to activation of Na+ /H+ exchange. Bicarbonates 63-67 glucose-6-phosphate isomerase Homo sapiens 153-156 29730171-7 2018 In addition, we recorded the effects of other strongly related short-chain monocarboxylates (l-lactate (10 mM) and the ketone body DL-beta-hydroxybutyrate (10 mM)) on ED and pHi. dl-beta-hydroxybutyrate 131-154 glucose-6-phosphate isomerase Homo sapiens 174-177 29748796-1 2018 In order to degrade the macromolecular pollutant of humic acid, the powder ordered mesoporous carbon (POMC, average pore diameter 4.29 nm) was first applied for preparing the granular OMC (GOMC, Phi x H = 4 x 3-6 mm) as electrodes in a continuous three-dimensional (3D) electrochemical system. Humic Substances 52-62 glucose-6-phosphate isomerase Homo sapiens 195-198 29748796-1 2018 In order to degrade the macromolecular pollutant of humic acid, the powder ordered mesoporous carbon (POMC, average pore diameter 4.29 nm) was first applied for preparing the granular OMC (GOMC, Phi x H = 4 x 3-6 mm) as electrodes in a continuous three-dimensional (3D) electrochemical system. Carbon 94-100 glucose-6-phosphate isomerase Homo sapiens 195-198 29748796-1 2018 In order to degrade the macromolecular pollutant of humic acid, the powder ordered mesoporous carbon (POMC, average pore diameter 4.29 nm) was first applied for preparing the granular OMC (GOMC, Phi x H = 4 x 3-6 mm) as electrodes in a continuous three-dimensional (3D) electrochemical system. pomc 102-106 glucose-6-phosphate isomerase Homo sapiens 195-198 29730171-9 2018 In parallel experiments, all three short-chain monocarboxylates (each n = 4) lowered the pHi of the neurons (n = 12) by 0.05-0.07 pH units which was temporally related to the reported changes in bioelectric activity. monocarboxylates 47-63 glucose-6-phosphate isomerase Homo sapiens 89-92 29706628-5 2018 Moreover, paclitaxel regulates a number of cancer-associated RNA alternative splicing events, including genes involved in cellular response to DNA damage stimulus, preassembly of GPI anchor in ER membrane, transcription, and DNA repair. Paclitaxel 10-20 glucose-6-phosphate isomerase Homo sapiens 179-182 29962845-6 2018 The goal of the present study was to evaluate the relationship between Phi, time, intracellular water content, and weight for head-and-neck cancer patients undergoing radiotherapy. Water 96-101 glucose-6-phosphate isomerase Homo sapiens 71-74 29962845-7 2018 The results demonstrate that Phi decreases with time and increases with intracellular water content and weight. Water 86-91 glucose-6-phosphate isomerase Homo sapiens 29-32 29743300-13 2018 We hypothesize that the imidazole group of His168 interacts with nearby Phe165 or other parts of hHV1 to transduce pHi into shifts of voltage-dependent gating. imidazole 24-33 glucose-6-phosphate isomerase Homo sapiens 115-118 29524788-4 2018 DESIGN: Microspectrofluorimetry technique with pH-sensitive fluorescent dye, BCECF, was used to detect pHi changes. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 77-82 glucose-6-phosphate isomerase Homo sapiens 103-106 29524788-8 2018 The pHi recovery following the induced-intracellular acidosis was blocked completely by removing [Na+]o, while only slowed (-63%) by adding HOE694 (a NHE1 specific inhibitor) in HEPES-buffered solution. 3-methylsulfonyl-4-piperidinobenzoyl guanidine 140-146 glucose-6-phosphate isomerase Homo sapiens 4-7 29524788-8 2018 The pHi recovery following the induced-intracellular acidosis was blocked completely by removing [Na+]o, while only slowed (-63%) by adding HOE694 (a NHE1 specific inhibitor) in HEPES-buffered solution. HEPES 178-183 glucose-6-phosphate isomerase Homo sapiens 4-7 29524788-10 2018 Both in HEPES-buffered and CO2/HCO3-buffered system solution, the pHi recovery after induced-intracellular alkalosis was entirely blocked by removing [Cl-]o. HEPES 8-13 glucose-6-phosphate isomerase Homo sapiens 66-69 29524788-10 2018 Both in HEPES-buffered and CO2/HCO3-buffered system solution, the pHi recovery after induced-intracellular alkalosis was entirely blocked by removing [Cl-]o. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 27-30 glucose-6-phosphate isomerase Homo sapiens 66-69 29524788-10 2018 Both in HEPES-buffered and CO2/HCO3-buffered system solution, the pHi recovery after induced-intracellular alkalosis was entirely blocked by removing [Cl-]o. Bicarbonates 31-35 glucose-6-phosphate isomerase Homo sapiens 66-69 29318675-2 2018 Herein, a 2D cyanamide-functionalized polyheptazine imide (NCN-PHI) is reported, which for the first time enables the synergistic coupling of two key functions of energy conversion within one single material: light harvesting and electrical energy storage. Cyanamide 13-22 glucose-6-phosphate isomerase Homo sapiens 63-66 29706896-4 2018 Our experimental results in HeLa cells expressing Cx36 show that changes in both pHi and [Mg2+]i affect junctional conductance (gj) in an interdependent manner; in other words, intracellular acidification cause increase or decay in gj depending on whether [Mg2+]i is high or low, respectively, and intracellular alkalization cause reduction in gj independently of [Mg2+]i. magnesium ion 257-261 glucose-6-phosphate isomerase Homo sapiens 81-84 29706896-4 2018 Our experimental results in HeLa cells expressing Cx36 show that changes in both pHi and [Mg2+]i affect junctional conductance (gj) in an interdependent manner; in other words, intracellular acidification cause increase or decay in gj depending on whether [Mg2+]i is high or low, respectively, and intracellular alkalization cause reduction in gj independently of [Mg2+]i. magnesium ion 257-261 glucose-6-phosphate isomerase Homo sapiens 81-84 29706896-6 2018 Using recombinant Cx36 we found that two glutamate residues in the N-terminus could be partly responsible for the observed interrelated effect of pHi and [Mg2+]i. Mutation of glutamate at position 8 attenuated the stimulatory effect of intracellular acidification at high [Mg2+]i, while mutation at position 12 and double mutation at both positions reversed stimulatory effect to inhibition. Glutamic Acid 41-50 glucose-6-phosphate isomerase Homo sapiens 146-149 29706896-6 2018 Using recombinant Cx36 we found that two glutamate residues in the N-terminus could be partly responsible for the observed interrelated effect of pHi and [Mg2+]i. Mutation of glutamate at position 8 attenuated the stimulatory effect of intracellular acidification at high [Mg2+]i, while mutation at position 12 and double mutation at both positions reversed stimulatory effect to inhibition. Glutamic Acid 175-184 glucose-6-phosphate isomerase Homo sapiens 146-149 29706896-6 2018 Using recombinant Cx36 we found that two glutamate residues in the N-terminus could be partly responsible for the observed interrelated effect of pHi and [Mg2+]i. Mutation of glutamate at position 8 attenuated the stimulatory effect of intracellular acidification at high [Mg2+]i, while mutation at position 12 and double mutation at both positions reversed stimulatory effect to inhibition. magnesium ion 273-277 glucose-6-phosphate isomerase Homo sapiens 146-149 29488255-6 2018 Importantly, the developed nanoprobe could successfully be applied for the detection of [Ca2+ ]i and pHi change in adenosine triphosphate (ATP) and ethylene glycol tetraacetic acid (EGTA) stimulation in living cells. Adenosine Triphosphate 115-137 glucose-6-phosphate isomerase Homo sapiens 101-104 29488255-6 2018 Importantly, the developed nanoprobe could successfully be applied for the detection of [Ca2+ ]i and pHi change in adenosine triphosphate (ATP) and ethylene glycol tetraacetic acid (EGTA) stimulation in living cells. Adenosine Triphosphate 139-142 glucose-6-phosphate isomerase Homo sapiens 101-104 29488255-6 2018 Importantly, the developed nanoprobe could successfully be applied for the detection of [Ca2+ ]i and pHi change in adenosine triphosphate (ATP) and ethylene glycol tetraacetic acid (EGTA) stimulation in living cells. Egtazic Acid 148-180 glucose-6-phosphate isomerase Homo sapiens 101-104 29488255-6 2018 Importantly, the developed nanoprobe could successfully be applied for the detection of [Ca2+ ]i and pHi change in adenosine triphosphate (ATP) and ethylene glycol tetraacetic acid (EGTA) stimulation in living cells. Egtazic Acid 182-186 glucose-6-phosphate isomerase Homo sapiens 101-104 29318675-2 2018 Herein, a 2D cyanamide-functionalized polyheptazine imide (NCN-PHI) is reported, which for the first time enables the synergistic coupling of two key functions of energy conversion within one single material: light harvesting and electrical energy storage. polyheptazine imide 38-57 glucose-6-phosphate isomerase Homo sapiens 63-66 29318675-3 2018 Photo-electrochemical measurements in aqueous electrolytes reveal the underlying mechanism of this "solar battery" material: the charge storage in NCN-PHI is based on the photoreduction of the carbon nitride backbone and charge compensation is realized by adsorption of alkali metal ions within the NCN-PHI layers and at the solution interface. cyanogen 193-207 glucose-6-phosphate isomerase Homo sapiens 151-154 28988317-11 2018 The results show concerted roles of the oppositely charged Lys and Glu in pCt for the ATP-dependent low basal activity and pHi sensitivity. Lysine 59-62 glucose-6-phosphate isomerase Homo sapiens 123-126 29858855-5 2018 The aim of this review is (a) to summarize epidemiological data confirming an association of PHI with an increased risk of a range of psychiatric disorders from childhood through adolescence to adulthood, (b) to present immunohistochemical findings on human autopsy material indicating vulnerability of the dopaminergic neurons of the human neonate to PHI that could predispose infant survivors of PHI to dopamine-related neurological and/or cognitive deficits in adulthood, and Dopamine 307-315 glucose-6-phosphate isomerase Homo sapiens 93-96 29137852-11 2018 Understanding causes of GPi-DBS-induced speech changes can improve DBS programming. dibromsalan 28-31 glucose-6-phosphate isomerase Homo sapiens 24-27 28988317-6 2018 Neutralization of cationic Lys (K330A) also eliminated the acidic pHi sensitivity of ITREK-2,i-o. Lysine 27-30 glucose-6-phosphate isomerase Homo sapiens 66-69 28988317-11 2018 The results show concerted roles of the oppositely charged Lys and Glu in pCt for the ATP-dependent low basal activity and pHi sensitivity. Glutamic Acid 67-70 glucose-6-phosphate isomerase Homo sapiens 123-126 28988317-11 2018 The results show concerted roles of the oppositely charged Lys and Glu in pCt for the ATP-dependent low basal activity and pHi sensitivity. Adenosine Triphosphate 86-89 glucose-6-phosphate isomerase Homo sapiens 123-126 29975935-1 2018 BACKGROUND/AIMS: To functionally characterize intracellular pH (pHi) regulating mechanisms, such as Na+-H+ exchanger (NHE) and Na+-HCO3- co-transporter (NBC), and further examine effects of ethanol on the pHi regulating mechanism in human oral epidermoid carcinoma (OEC-M1) cells. Ethanol 190-197 glucose-6-phosphate isomerase Homo sapiens 64-67 29975935-3 2018 Changes of pHi were detected by microspectrofluroimetry with a pH-sensitive fluorescent dye, BCECF. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 93-98 glucose-6-phosphate isomerase Homo sapiens 11-14 29975935-6 2018 CONCLUSIONS: Ethanol affects pHi in a concentration-dependent manner by changing function and expression of NHE1 and NBC isoforms in OEC-M1 cells. Ethanol 13-20 glucose-6-phosphate isomerase Homo sapiens 29-32 28869819-0 2017 Rational optimization of the mannoside building block for automated electrochemical assembly of the core trisaccharide of GPI anchor oligosaccharides. Mannosides 29-38 glucose-6-phosphate isomerase Homo sapiens 122-125 29246604-9 2017 RESULTS: Bilateral GPi DBS improved the BFMDRS total movement score by 65% at short-term follow-up and by 53% at long-term follow-up when compared to baseline scores. dibromsalan 23-26 glucose-6-phosphate isomerase Homo sapiens 19-22 29246604-13 2017 CONCLUSION: Our results showed that bilateral GPi DBS in craniocervical dystonia is effective and safe. dibromsalan 50-53 glucose-6-phosphate isomerase Homo sapiens 46-49 29050473-7 2017 Taking advantages of these merits, we employ Eu@SiNRs for the visualization of the cytoplasmic alkalization process mediated by nigericin in living cells, for around 30 min without interruption, revealing important information for understanding the dynamic process of pHi fluctuations. Nigericin 128-137 glucose-6-phosphate isomerase Homo sapiens 268-271 28869819-0 2017 Rational optimization of the mannoside building block for automated electrochemical assembly of the core trisaccharide of GPI anchor oligosaccharides. Trisaccharides 105-118 glucose-6-phosphate isomerase Homo sapiens 122-125 28869819-0 2017 Rational optimization of the mannoside building block for automated electrochemical assembly of the core trisaccharide of GPI anchor oligosaccharides. Oligosaccharides 133-149 glucose-6-phosphate isomerase Homo sapiens 122-125 28869819-2 2017 The optimized building block in hand was used to prepare the core trisaccharide of GPI anchor oligosaccharides. Trisaccharides 66-79 glucose-6-phosphate isomerase Homo sapiens 83-86 28869819-2 2017 The optimized building block in hand was used to prepare the core trisaccharide of GPI anchor oligosaccharides. Oligosaccharides 94-110 glucose-6-phosphate isomerase Homo sapiens 83-86 28590521-8 2017 The GPi reemerged in the late 1990s as a major stereotactic target for DBS in dystonia and, recently, in Tourette syndrome. dibromsalan 71-74 glucose-6-phosphate isomerase Homo sapiens 4-7 29152106-4 2017 We disrupted the upstream glycolytic enzyme, glucose-6-phosphate isomerase (GPI), to allow cells to re-route glucose-6-phosphate flux into the pentose-phosphate branch. Glucose-6-Phosphate 45-64 glucose-6-phosphate isomerase Homo sapiens 76-79 29152106-4 2017 We disrupted the upstream glycolytic enzyme, glucose-6-phosphate isomerase (GPI), to allow cells to re-route glucose-6-phosphate flux into the pentose-phosphate branch. Pentoses 143-150 glucose-6-phosphate isomerase Homo sapiens 45-74 29152106-4 2017 We disrupted the upstream glycolytic enzyme, glucose-6-phosphate isomerase (GPI), to allow cells to re-route glucose-6-phosphate flux into the pentose-phosphate branch. Pentoses 143-150 glucose-6-phosphate isomerase Homo sapiens 76-79 29152106-4 2017 We disrupted the upstream glycolytic enzyme, glucose-6-phosphate isomerase (GPI), to allow cells to re-route glucose-6-phosphate flux into the pentose-phosphate branch. Phosphates 55-64 glucose-6-phosphate isomerase Homo sapiens 76-79 29152106-5 2017 Indeed, GPI-KO severely reduced glucose consumption and suppressed lactic acid secretion, which reprogrammed these cells to rely on oxidative phosphorylation and mitochondrial ATP production to maintain viability. Lactic Acid 67-78 glucose-6-phosphate isomerase Homo sapiens 8-11 29152106-5 2017 Indeed, GPI-KO severely reduced glucose consumption and suppressed lactic acid secretion, which reprogrammed these cells to rely on oxidative phosphorylation and mitochondrial ATP production to maintain viability. Adenosine Triphosphate 176-179 glucose-6-phosphate isomerase Homo sapiens 8-11 28874603-2 2017 We found that increased pHi enabled the tumorigenic behaviors caused by somatic arginine-to-histidine mutations, which are frequent in cancer and confer pH sensing not seen with wild-type proteins. Arginine 80-88 glucose-6-phosphate isomerase Homo sapiens 24-27 28874603-2 2017 We found that increased pHi enabled the tumorigenic behaviors caused by somatic arginine-to-histidine mutations, which are frequent in cancer and confer pH sensing not seen with wild-type proteins. Histidine 92-101 glucose-6-phosphate isomerase Homo sapiens 24-27 28874603-6 2017 An Arg-to-His, but not Arg-to-Lys, mutation in the transcription factor p53 (p53-R273H) decreased its transcriptional activity and attenuated the DNA damage response in fibroblasts and breast cancer cells with high pHi. Arginine 3-6 glucose-6-phosphate isomerase Homo sapiens 215-218 28130898-4 2017 The optimum pHi was 7 for TiCl4, 5.5 for Ti(SO4)2, and 7 for Al2(SO4)3. titanium tetrachloride 26-31 glucose-6-phosphate isomerase Homo sapiens 12-15 28130898-4 2017 The optimum pHi was 7 for TiCl4, 5.5 for Ti(SO4)2, and 7 for Al2(SO4)3. Titanium(IV) sulfate 41-49 glucose-6-phosphate isomerase Homo sapiens 12-15 28130898-4 2017 The optimum pHi was 7 for TiCl4, 5.5 for Ti(SO4)2, and 7 for Al2(SO4)3. aluminum sulfate 61-70 glucose-6-phosphate isomerase Homo sapiens 12-15 28541706-1 2017 A new strategy has been developed for GPI glycan-peptide conjugate synthesis based upon a traceless Staudinger reaction between a peptide phosphinothioester and a GPI glycan azide. glycan azide 167-179 glucose-6-phosphate isomerase Homo sapiens 38-41 28541706-1 2017 A new strategy has been developed for GPI glycan-peptide conjugate synthesis based upon a traceless Staudinger reaction between a peptide phosphinothioester and a GPI glycan azide. glycan azide 167-179 glucose-6-phosphate isomerase Homo sapiens 163-166 28541706-2 2017 The strategy was first studied and optimized with simple peptides and GPI glycans, which offered excellent yields of the desired conjugates in both organic and aqueous solvents. Polysaccharides 74-81 glucose-6-phosphate isomerase Homo sapiens 70-73 28541706-3 2017 It was then used to successfully synthesize an analogue of the human CD52 antigen containing the whole CD52 peptide sequence and the conserved trimannose motif of all GPI anchors. trimannose 143-153 glucose-6-phosphate isomerase Homo sapiens 167-170 28827680-2 2017 The present study demonstrates the relationships between the intracellular pH (pHi), cell bioenergetics and the response of cervical cancer to cisplatin. Cisplatin 143-152 glucose-6-phosphate isomerase Homo sapiens 79-82 28605596-5 2017 Finally, detailed time-resolved spectral analysis of P, carotenoid, and BB (Phi in the M182HL mutant) reveals that the triplet state of the carotenoid is coupled fairly strongly to the bridging intermediate BB in wild-type and Phi in the M182HL mutant, a fact that is probably responsible for the lack of any obvious intermediate 3BB/3Phi transient formation during triplet energy transfer. boeravinone B 72-74 glucose-6-phosphate isomerase Homo sapiens 76-79 28605596-5 2017 Finally, detailed time-resolved spectral analysis of P, carotenoid, and BB (Phi in the M182HL mutant) reveals that the triplet state of the carotenoid is coupled fairly strongly to the bridging intermediate BB in wild-type and Phi in the M182HL mutant, a fact that is probably responsible for the lack of any obvious intermediate 3BB/3Phi transient formation during triplet energy transfer. Carotenoids 140-150 glucose-6-phosphate isomerase Homo sapiens 76-79 28605596-5 2017 Finally, detailed time-resolved spectral analysis of P, carotenoid, and BB (Phi in the M182HL mutant) reveals that the triplet state of the carotenoid is coupled fairly strongly to the bridging intermediate BB in wild-type and Phi in the M182HL mutant, a fact that is probably responsible for the lack of any obvious intermediate 3BB/3Phi transient formation during triplet energy transfer. Carotenoids 140-150 glucose-6-phosphate isomerase Homo sapiens 227-230 28590521-9 2017 Lately, lesioning of the GPI is being proposed to treat refractory status dystonicus or to treat DBS withdrawal syndrome in PD patients. dibromsalan 97-100 glucose-6-phosphate isomerase Homo sapiens 25-28 28599497-7 2017 In A549-xenografted nude mice treated with sunitinib or cetuximab, a decrease in the plasma AMF concentration was accompanied by a reduction in tumor weight, suggesting an association between the plasma AMF concentration and anticancer activity. Sunitinib 43-52 glucose-6-phosphate isomerase Homo sapiens 92-95 28537279-0 2017 Synthesis of biotin-labelled core glycans of GPI anchors and their application in the study of GPI interaction with pore-forming bacterial toxins. Biotin 13-19 glucose-6-phosphate isomerase Homo sapiens 45-48 28537279-0 2017 Synthesis of biotin-labelled core glycans of GPI anchors and their application in the study of GPI interaction with pore-forming bacterial toxins. Biotin 13-19 glucose-6-phosphate isomerase Homo sapiens 95-98 28537279-0 2017 Synthesis of biotin-labelled core glycans of GPI anchors and their application in the study of GPI interaction with pore-forming bacterial toxins. Polysaccharides 34-41 glucose-6-phosphate isomerase Homo sapiens 45-48 28537279-0 2017 Synthesis of biotin-labelled core glycans of GPI anchors and their application in the study of GPI interaction with pore-forming bacterial toxins. Polysaccharides 34-41 glucose-6-phosphate isomerase Homo sapiens 95-98 28537279-1 2017 A convergent strategy was developed for the first-time synthesis of biotin-labeled GPI core glycans. Biotin 68-74 glucose-6-phosphate isomerase Homo sapiens 83-86 28537279-1 2017 A convergent strategy was developed for the first-time synthesis of biotin-labeled GPI core glycans. Polysaccharides 92-99 glucose-6-phosphate isomerase Homo sapiens 83-86 28537279-2 2017 These GPI conjugates are useful for various biological studies showcased by their application in the scrutiny of pore-forming bacterial toxin-GPI interaction, revealing that the phosphate group at the GPI inositol 1-O-position had a significant impact on GPI-toxin binding. Phosphates 178-187 glucose-6-phosphate isomerase Homo sapiens 6-9 28537279-2 2017 These GPI conjugates are useful for various biological studies showcased by their application in the scrutiny of pore-forming bacterial toxin-GPI interaction, revealing that the phosphate group at the GPI inositol 1-O-position had a significant impact on GPI-toxin binding. Phosphates 178-187 glucose-6-phosphate isomerase Homo sapiens 142-145 28537279-2 2017 These GPI conjugates are useful for various biological studies showcased by their application in the scrutiny of pore-forming bacterial toxin-GPI interaction, revealing that the phosphate group at the GPI inositol 1-O-position had a significant impact on GPI-toxin binding. Phosphates 178-187 glucose-6-phosphate isomerase Homo sapiens 142-145 28537279-2 2017 These GPI conjugates are useful for various biological studies showcased by their application in the scrutiny of pore-forming bacterial toxin-GPI interaction, revealing that the phosphate group at the GPI inositol 1-O-position had a significant impact on GPI-toxin binding. Phosphates 178-187 glucose-6-phosphate isomerase Homo sapiens 142-145 28290739-7 2017 RESULTS: Reliable estimates were observed with at least 2 days (G & Phi = .910) and 12 hours (G = .806, Phi = .803) at the ankle, and with at least 3 days (G & Phi = .906) and 15 hours (G = .802, Phi = .800) at the wrist. g & 64-70 glucose-6-phosphate isomerase Homo sapiens 72-75 28599497-7 2017 In A549-xenografted nude mice treated with sunitinib or cetuximab, a decrease in the plasma AMF concentration was accompanied by a reduction in tumor weight, suggesting an association between the plasma AMF concentration and anticancer activity. Sunitinib 43-52 glucose-6-phosphate isomerase Homo sapiens 203-206 28422979-9 2017 CAGE revealed that 4 genes associated with glucose metabolism (GPI, G6PD, PKM2, and GAPDH) and 5 associated with the cell cycle (ANLN, PTTG1, CIT, KPNA2, and CDC25A) were positively correlated with SUVmax, although expression levels were lower in EGFR-mutated than in wild-type tumors. Glucose 43-50 glucose-6-phosphate isomerase Homo sapiens 63-66 28120309-5 2017 Upon improving the intracellular TDP-L-rhamnose pool by knocking out the chromosomal glucose phosphate isomerase (pgi) and D-glucose-6-phosphate dehydrogenase (zwf) deletion along with the overexpression of rhamnose biosynthetic pathway increases the biotransformation product, ATN with total conversion of ~49.5 +- 1.67% from 100 microM of taxifolin. tdp-l-rhamnose 33-47 glucose-6-phosphate isomerase Homo sapiens 114-117 27623507-2 2017 In tumors, microenvironmental changes, like in lactate metabolism, lead to altered intra- and extracellular pH (pHi , pHe ) and vice versa. Lactic Acid 47-54 glucose-6-phosphate isomerase Homo sapiens 112-115 28297686-4 2017 He experienced the successful tremor relief after unipolar DBS in the globus pallidus internus(GPi)with Vercise<sup>TM</sup> but complained of dysarthria. dibromsalan 59-62 glucose-6-phosphate isomerase Homo sapiens 95-98 28297686-10 2017 A fine current steering of Vercise<sup>TM</sup> DBS is very useful in both, the early and late phases of GPi-DBS for dystonic syndrome. dibromsalan 60-63 glucose-6-phosphate isomerase Homo sapiens 117-120 27779763-14 2017 However, mutations that disrupt the interaction of ATP with ATP-binding site 1, including K464A, D572N and the CF-associated mutation G1349D all abolished the prolongation of taucf at pHi 6.3. Adenosine Triphosphate 51-54 glucose-6-phosphate isomerase Homo sapiens 184-187 27779763-14 2017 However, mutations that disrupt the interaction of ATP with ATP-binding site 1, including K464A, D572N and the CF-associated mutation G1349D all abolished the prolongation of taucf at pHi 6.3. Adenosine Triphosphate 60-63 glucose-6-phosphate isomerase Homo sapiens 184-187 28959869-4 2017 The chromophoric groups, the changes of secondary structure and the molecular docking simulations revealed that the active pocket formed by Cys281-Lys-Asn-Lys-Glu-Lys-Lys287 and Leu313-Ala-Phe-Trp316 of P.rbeta2-GPI-DV and the -COOH carboxyl of oxLig-1 were the key for binding. Lysine 147-150 glucose-6-phosphate isomerase Homo sapiens 212-215 28959869-4 2017 The chromophoric groups, the changes of secondary structure and the molecular docking simulations revealed that the active pocket formed by Cys281-Lys-Asn-Lys-Glu-Lys-Lys287 and Leu313-Ala-Phe-Trp316 of P.rbeta2-GPI-DV and the -COOH carboxyl of oxLig-1 were the key for binding. Asparagine 151-154 glucose-6-phosphate isomerase Homo sapiens 212-215 28959869-4 2017 The chromophoric groups, the changes of secondary structure and the molecular docking simulations revealed that the active pocket formed by Cys281-Lys-Asn-Lys-Glu-Lys-Lys287 and Leu313-Ala-Phe-Trp316 of P.rbeta2-GPI-DV and the -COOH carboxyl of oxLig-1 were the key for binding. Lysine 155-158 glucose-6-phosphate isomerase Homo sapiens 212-215 28959869-4 2017 The chromophoric groups, the changes of secondary structure and the molecular docking simulations revealed that the active pocket formed by Cys281-Lys-Asn-Lys-Glu-Lys-Lys287 and Leu313-Ala-Phe-Trp316 of P.rbeta2-GPI-DV and the -COOH carboxyl of oxLig-1 were the key for binding. Glutamic Acid 159-162 glucose-6-phosphate isomerase Homo sapiens 212-215 28959869-4 2017 The chromophoric groups, the changes of secondary structure and the molecular docking simulations revealed that the active pocket formed by Cys281-Lys-Asn-Lys-Glu-Lys-Lys287 and Leu313-Ala-Phe-Trp316 of P.rbeta2-GPI-DV and the -COOH carboxyl of oxLig-1 were the key for binding. Lysine 155-158 glucose-6-phosphate isomerase Homo sapiens 212-215 28959869-4 2017 The chromophoric groups, the changes of secondary structure and the molecular docking simulations revealed that the active pocket formed by Cys281-Lys-Asn-Lys-Glu-Lys-Lys287 and Leu313-Ala-Phe-Trp316 of P.rbeta2-GPI-DV and the -COOH carboxyl of oxLig-1 were the key for binding. Phenylalanine 189-192 glucose-6-phosphate isomerase Homo sapiens 212-215 28959869-5 2017 P.rbeta2-GPI combined with ox-LDL via the fifth functional domain and the -COOH group. Carbonic Acid 75-79 glucose-6-phosphate isomerase Homo sapiens 9-12 28120309-5 2017 Upon improving the intracellular TDP-L-rhamnose pool by knocking out the chromosomal glucose phosphate isomerase (pgi) and D-glucose-6-phosphate dehydrogenase (zwf) deletion along with the overexpression of rhamnose biosynthetic pathway increases the biotransformation product, ATN with total conversion of ~49.5 +- 1.67% from 100 microM of taxifolin. astilbin 278-281 glucose-6-phosphate isomerase Homo sapiens 114-117 28120309-5 2017 Upon improving the intracellular TDP-L-rhamnose pool by knocking out the chromosomal glucose phosphate isomerase (pgi) and D-glucose-6-phosphate dehydrogenase (zwf) deletion along with the overexpression of rhamnose biosynthetic pathway increases the biotransformation product, ATN with total conversion of ~49.5 +- 1.67% from 100 microM of taxifolin. taxifolin 341-350 glucose-6-phosphate isomerase Homo sapiens 114-117 27629415-5 2016 The pHi influences cytokine secretion, activities of membrane-associated enzymes, ion transport, and other effector signaling molecules such as ATP and Ca2+ levels. Adenosine Triphosphate 144-147 glucose-6-phosphate isomerase Homo sapiens 4-7 28067317-2 2017 Herein we found the enrichment of G6PI in microvascular endothelial cells of synovial tissue in RA patients, where a 3% O2 hypoxia environment has been identified. Oxygen 120-122 glucose-6-phosphate isomerase Homo sapiens 34-38 28067317-3 2017 In order to determine the correlation between the high G6PI level and the low oxygen concentration in RA, a hypoxia condition (~3% O2) in vitro was applied to mimic the RA environment in vivo. Oxygen 78-84 glucose-6-phosphate isomerase Homo sapiens 55-59 28467979-9 2017 RESULTS: A 48 hour treatment with Ellagic acid (20 microM) significantly decreased NHE1 transcript levels by 75%, NHE1 protein abundance by 95%, pHi from 7.24 +- 0.01 to 7.02 +- 0.01, Na+/H+ exchanger activity by 77%, forward scatter by 10%, ROS by 82%, glucose uptake by 58%, and lactate release by 15%. Ellagic Acid 34-46 glucose-6-phosphate isomerase Homo sapiens 145-148 28467979-10 2017 CONCLUSION: Ellagic acid (20microM) markedly down-regulates ROS formation and NHE1 expression leading to decreased Na+/H+ exchanger activity, pHi, glucose uptake and lactate release in endometrial cancer cells. Ellagic Acid 12-24 glucose-6-phosphate isomerase Homo sapiens 142-145 28053225-9 2017 The concanamycin A-sensitive pHi recovery was stimulated by cAMP at pH 7.4 in vitro, but intraventricular infusion of the membrane-permeant cAMP analog 8-CPT-cAMP did not result in trafficking of the V-ATPase. concanamycin A 4-18 glucose-6-phosphate isomerase Homo sapiens 29-32 28053225-9 2017 The concanamycin A-sensitive pHi recovery was stimulated by cAMP at pH 7.4 in vitro, but intraventricular infusion of the membrane-permeant cAMP analog 8-CPT-cAMP did not result in trafficking of the V-ATPase. Cyclic AMP 60-64 glucose-6-phosphate isomerase Homo sapiens 29-32 28053225-9 2017 The concanamycin A-sensitive pHi recovery was stimulated by cAMP at pH 7.4 in vitro, but intraventricular infusion of the membrane-permeant cAMP analog 8-CPT-cAMP did not result in trafficking of the V-ATPase. Cyclic AMP 140-144 glucose-6-phosphate isomerase Homo sapiens 29-32 28053225-9 2017 The concanamycin A-sensitive pHi recovery was stimulated by cAMP at pH 7.4 in vitro, but intraventricular infusion of the membrane-permeant cAMP analog 8-CPT-cAMP did not result in trafficking of the V-ATPase. 8-cpt 152-157 glucose-6-phosphate isomerase Homo sapiens 29-32 28053225-9 2017 The concanamycin A-sensitive pHi recovery was stimulated by cAMP at pH 7.4 in vitro, but intraventricular infusion of the membrane-permeant cAMP analog 8-CPT-cAMP did not result in trafficking of the V-ATPase. Cyclic AMP 140-144 glucose-6-phosphate isomerase Homo sapiens 29-32 27991590-3 2016 The polarization dependence in transmission can be flexibly tailored by adjusting the intersection angle, following a cos2 (Phi + theta/2) angular dependence on polarization angle Phi. cos2 118-122 glucose-6-phosphate isomerase Homo sapiens 124-127 27991590-3 2016 The polarization dependence in transmission can be flexibly tailored by adjusting the intersection angle, following a cos2 (Phi + theta/2) angular dependence on polarization angle Phi. cos2 118-122 glucose-6-phosphate isomerase Homo sapiens 180-183 27934889-4 2016 Herein, we have developed a self-ratiometric luminescence nanoprobe based on forster resonant energy transfer (FRET) for probing pHi, in which pH-sensitive fluorescein isothiocyanate (FITC) and upconversion nanoparticles (UCNPs) were served as energy acceptor and donor, respectively. Fluorescein-5-isothiocyanate 156-182 glucose-6-phosphate isomerase Homo sapiens 129-132 27934889-4 2016 Herein, we have developed a self-ratiometric luminescence nanoprobe based on forster resonant energy transfer (FRET) for probing pHi, in which pH-sensitive fluorescein isothiocyanate (FITC) and upconversion nanoparticles (UCNPs) were served as energy acceptor and donor, respectively. Fluorescein-5-isothiocyanate 184-188 glucose-6-phosphate isomerase Homo sapiens 129-132 26864256-4 2016 This results in the generation of a pH gradient between the low extracellular pH that is acidic (pHe) and the higher cytosolic alkaline or near neutral pH (pHi) environments that are tightly regulated by the overexpression of several pumps and ion channels (e.g. NHE-1, MCT-1, V-ATPase, CA9, and CA12). Phenylalanine 97-100 glucose-6-phosphate isomerase Homo sapiens 156-159 27773735-4 2017 Basal pHi and pHi recovery (following a NH4Cl pulse) was higher in haOC and A2780, compared with HOSE cells. Ammonium Chloride 40-45 glucose-6-phosphate isomerase Homo sapiens 14-17 27773735-5 2017 Zoniporide (NHE1 inhibitor) caused intracellular acidification and pHi recovery was independent of intracellular buffer capacity, but reduced in NHE1 knockdown A2780 cells. zoniporide 0-10 glucose-6-phosphate isomerase Homo sapiens 67-70 28222424-7 2017 RESULTS: A 24 hours treatment with 1,25(OH)2D3 (100 nM) significantly increased cytosolic pH (pHi), significantly decreased Na+/H+ exchanger activity, NHE1 and MCT4 transcript levels as well as protein abundance and significantly increased lactate concentration in the supernatant. Calcitriol 35-46 glucose-6-phosphate isomerase Homo sapiens 94-97 27823565-6 2017 The inhibitors of glycogen phosphorylase (GPi) control the blood glucose level effectively by inhibiting the hepatic GP (hGP). Glucose 65-72 glucose-6-phosphate isomerase Homo sapiens 42-45 27629415-8 2016 Th cell subsets were characterized by flow cytometry and pHi was measured using 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein-acetoxymethyl ester (BCECF-AM) dye. 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein-acetoxymethyl ester 80-155 glucose-6-phosphate isomerase Homo sapiens 57-60 27595783-1 2016 BACKGROUND: Human anion exchanger 1 and 2 (AE1 and AE2) mediate the exchange of Cl(-)/HCO3 (-) across the plasma membrane and regulate intracellular pH (pHi). Bicarbonates 86-90 glucose-6-phosphate isomerase Homo sapiens 153-156 27592369-8 2016 We found that serum GPI and RF concentrations showed a significant positive correlation with TRAF1 concentrations respectively (r=0.767, p<0.001; r=0.365, p=0.001), while CCP concentration showed no significant correlation. ccp 174-177 glucose-6-phosphate isomerase Homo sapiens 20-23 27522492-2 2016 GPI compete with a wider use of ADP inhibitors and novel anticoagulant drugs although GPI use has greatly narrowed. Adenosine Diphosphate 32-35 glucose-6-phosphate isomerase Homo sapiens 0-3 27016482-0 2016 Non-basic amino acids in the ROMK1 channels via an appropriate distance modulate PIP2 regulated pHi-gating. Amino Acids, Basic 4-21 glucose-6-phosphate isomerase Homo sapiens 96-99 27450748-2 2016 AIM: To study the prevalence of neurogenic bowel dysfunction (NBD) in those patients, and to assess its severity with the Patient Global Impression of Severity (PGI-S). Sulfur 151-152 glucose-6-phosphate isomerase Homo sapiens 161-164 27630987-6 2016 The intracellular pH (pHi) measured using the fluorescent ratio dye 2",7"-bis(2-carboxyethyl)-5(6)-155 carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of acidification when proton efflux was inhibited. 2",7"-bis(2-carboxyethyl)-5(6)-155 68-102 glucose-6-phosphate isomerase Homo sapiens 18-20 27630987-6 2016 The intracellular pH (pHi) measured using the fluorescent ratio dye 2",7"-bis(2-carboxyethyl)-5(6)-155 carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of acidification when proton efflux was inhibited. 2",7"-bis(2-carboxyethyl)-5(6)-155 68-102 glucose-6-phosphate isomerase Homo sapiens 22-25 27630987-6 2016 The intracellular pH (pHi) measured using the fluorescent ratio dye 2",7"-bis(2-carboxyethyl)-5(6)-155 carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of acidification when proton efflux was inhibited. 6-carboxyfluorescein 103-121 glucose-6-phosphate isomerase Homo sapiens 18-20 27630987-6 2016 The intracellular pH (pHi) measured using the fluorescent ratio dye 2",7"-bis(2-carboxyethyl)-5(6)-155 carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of acidification when proton efflux was inhibited. 6-carboxyfluorescein 103-121 glucose-6-phosphate isomerase Homo sapiens 22-25 27630987-6 2016 The intracellular pH (pHi) measured using the fluorescent ratio dye 2",7"-bis(2-carboxyethyl)-5(6)-155 carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of acidification when proton efflux was inhibited. bcecf 123-128 glucose-6-phosphate isomerase Homo sapiens 18-20 27630987-6 2016 The intracellular pH (pHi) measured using the fluorescent ratio dye 2",7"-bis(2-carboxyethyl)-5(6)-155 carboxyfluorescein (BCECF) revealed expected differences between normal and cancer cells (low and high, respectively), and a mixed distribution in the MINO cells, with a subset of cells in the MINO having an increased rate of acidification when proton efflux was inhibited. bcecf 123-128 glucose-6-phosphate isomerase Homo sapiens 22-25 27441097-4 2016 CASE REPORT: A 24-year-old male underwent left Gpi DBS for medically refractory HT. dibromsalan 51-54 glucose-6-phosphate isomerase Homo sapiens 47-50 27035646-5 2016 In the current study, pHi and NHE activity were analyzed using the pHi-sensitive dye BCECF-AM. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 85-93 glucose-6-phosphate isomerase Homo sapiens 22-25 27035646-5 2016 In the current study, pHi and NHE activity were analyzed using the pHi-sensitive dye BCECF-AM. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 85-93 glucose-6-phosphate isomerase Homo sapiens 67-70 27035646-6 2016 Using cariporide (an NHE1-specific inhibitor) and EIPA (an NHE nonspecific inhibitor), the current study demonstrated that it was NHE1, not the other NHE isoforms, that was important in regulating pHi homeostasis in astrocytes during CoCl2 treatment. ethylisopropylamiloride 50-54 glucose-6-phosphate isomerase Homo sapiens 197-200 27035646-7 2016 Additionally, the present study observed that, during the early period of CoCl2 treatment (the first 2 h), NHE1 activity and pHi dropped immediately, and NHE1 mRNA expression was reduced compared with control levels, whereas expression levels of the NHE1 protein had not yet changed. cobaltous chloride 74-79 glucose-6-phosphate isomerase Homo sapiens 125-128 27035646-8 2016 In the later period of CoCl2 treatment, NHE1 activity and pHi significantly increased compared with the control levels, as did the mRNA and protein expression levels of NHE1. cobaltous chloride 23-28 glucose-6-phosphate isomerase Homo sapiens 58-61 27035646-9 2016 Furthermore, the cell viability and injury of astrocytes was not changed during the initial 8 h of CoCl2 treatment; their deterioration was associated with the higher levels of pHi and NHE1 activity. cobaltous chloride 99-104 glucose-6-phosphate isomerase Homo sapiens 177-180 27035646-10 2016 The current study concluded that NHE1 activity and pHi homeostasis are regulated by CoCl2 treatment in a time-dependent manner in astrocytes, and may be responsible for the changes in cell viability and injury observed under hypoxia-mimetic conditions induced by CoCl2 treatment. cobaltous chloride 84-89 glucose-6-phosphate isomerase Homo sapiens 51-54 27035646-10 2016 The current study concluded that NHE1 activity and pHi homeostasis are regulated by CoCl2 treatment in a time-dependent manner in astrocytes, and may be responsible for the changes in cell viability and injury observed under hypoxia-mimetic conditions induced by CoCl2 treatment. cobaltous chloride 263-268 glucose-6-phosphate isomerase Homo sapiens 51-54 26972028-1 2016 UNLABELLED: Glycosylphosphatidylinositol anchored proteins (GPI-APs) in the outer leaflet of the membrane microdomains, commonly referred to as lipid rafts, play important roles in many biological processes such as signal transduction, cell adhesion, protein trafficking, and antigen presentation. Glycosylphosphatidylinositols 12-40 glucose-6-phosphate isomerase Homo sapiens 60-63 26972028-12 2016 To improve the feasibility of large-scale site-specific analysis of GPI anchoring, we developed a method for identification of GPI-anchored peptides by combining titanium dioxide-based affinity purification with hydrogen fluoride treatment. titanium dioxide 162-178 glucose-6-phosphate isomerase Homo sapiens 127-130 26972028-12 2016 To improve the feasibility of large-scale site-specific analysis of GPI anchoring, we developed a method for identification of GPI-anchored peptides by combining titanium dioxide-based affinity purification with hydrogen fluoride treatment. Hydrofluoric Acid 212-229 glucose-6-phosphate isomerase Homo sapiens 127-130 27016482-2 2016 Although several residues were reported to be involved in the regulation of pHi associated with PIP2 interaction, the detailed molecular mechanism remains unclear. Phosphatidylinositol 4,5-Diphosphate 96-100 glucose-6-phosphate isomerase Homo sapiens 76-79 27526153-7 2016 The subjects pulled down a rope with the adjusted weight attached to the ankle at a frequency of 0.5 Hz for 380 s. Intracellular pH (pHi) was calculated from the median chemical shift of the inorganic phosphate (Pi) peak relative to phosphocreatine (PCr). Phosphates 191-210 glucose-6-phosphate isomerase Homo sapiens 133-136 26936801-1 2016 Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) is secreted by tumors and influences tumor growth and metastasis. Prostaglandins I 52-55 glucose-6-phosphate isomerase Homo sapiens 0-24 26998091-8 2016 Lactate influx into the tumor microenvironment produced an acidic pHi in colon cancer cells. Lactic Acid 0-7 glucose-6-phosphate isomerase Homo sapiens 66-69 26593072-1 2016 Glycosylphosphatidylinositol-anchored proteins (GPI-APs) contain a covalently linked GPI anchor located on outer cell membranes. Glycosylphosphatidylinositols 0-28 glucose-6-phosphate isomerase Homo sapiens 48-51 26593072-1 2016 Glycosylphosphatidylinositol-anchored proteins (GPI-APs) contain a covalently linked GPI anchor located on outer cell membranes. Glycosylphosphatidylinositols 0-28 glucose-6-phosphate isomerase Homo sapiens 85-88 26611690-1 2016 BACKGROUND: Dystonia has been treated well using deep brain stimulation at the globus pallidus internus (GPi DBS). dibromsalan 109-112 glucose-6-phosphate isomerase Homo sapiens 105-108 26611690-9 2016 CONCLUSIONS: The clinical outcome of phasic-type cervical dystonia is more favorable than that of tonic-type cervical dystonia following GPi DBS. dibromsalan 141-144 glucose-6-phosphate isomerase Homo sapiens 137-140 27526153-7 2016 The subjects pulled down a rope with the adjusted weight attached to the ankle at a frequency of 0.5 Hz for 380 s. Intracellular pH (pHi) was calculated from the median chemical shift of the inorganic phosphate (Pi) peak relative to phosphocreatine (PCr). Phosphocreatine 233-248 glucose-6-phosphate isomerase Homo sapiens 133-136 24697727-14 2016 In the in vitro study, the pHi increased immediately after the addition of SP. sp 75-77 glucose-6-phosphate isomerase Homo sapiens 27-30 24697727-7 2016 Intracellular pH (pHi) was fluorimetrically measured after the addition of SP. sp 75-77 glucose-6-phosphate isomerase Homo sapiens 18-21 26713849-14 2015 Thus, incubation of T84 cells with STa results in reduced NHE4 activity leading to a lower capacity of pHi recovery requiring cAMP, but not cGMP. Cyclic AMP 126-130 glucose-6-phosphate isomerase Homo sapiens 103-106 26713849-14 2015 Thus, incubation of T84 cells with STa results in reduced NHE4 activity leading to a lower capacity of pHi recovery requiring cAMP, but not cGMP. sta 35-38 glucose-6-phosphate isomerase Homo sapiens 103-106 30873452-12 2015 Moreover, tBHP significantly increased intracellular pH (pHi), decreased extracellular pH (pHe) and induced upregulation of ERK, MMP2, and MMP9. tert-Butylhydroperoxide 10-14 glucose-6-phosphate isomerase Homo sapiens 57-60 26713849-4 2015 pHi was assayed by the NH4Cl pulse technique and measured by fluorescence microscopy in BCECF-preloaded cells. Ammonium Chloride 23-28 glucose-6-phosphate isomerase Homo sapiens 0-3 26713849-4 2015 pHi was assayed by the NH4Cl pulse technique and measured by fluorescence microscopy in BCECF-preloaded cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 88-93 glucose-6-phosphate isomerase Homo sapiens 0-3 26713849-8 2015 STa and HOE-694 caused comparable reduction in dpHi/dt and JH+ (~63%), without altering basal pHi (range 7.144-7.172). sta 0-3 glucose-6-phosphate isomerase Homo sapiens 48-51 26713849-8 2015 STa and HOE-694 caused comparable reduction in dpHi/dt and JH+ (~63%), without altering basal pHi (range 7.144-7.172). 4-hydroxy-2-octenal 8-11 glucose-6-phosphate isomerase Homo sapiens 48-51 26577834-10 2015 (iv) EtOH induced a biphasic, concentration-dependent change in resting pHi (+0.25 pH unit at 100 mM but only +0.05 pH unit at 300 mM) in bicarbonate-buffered solution, while caused a concentration-dependent decrease in resting pHi (-0.06 pH unit at 300 mM) in HEPES-buffered solution. HEPES 261-266 glucose-6-phosphate isomerase Homo sapiens 72-75 26470817-2 2015 Failures in pHi homeostasis could alter critical cellular functions such as water transport and cell volume. Water 76-81 glucose-6-phosphate isomerase Homo sapiens 12-15 26577834-3 2015 The aims of the study were to identify the possible transmembrane pHi regulators and to explore the effects of ethanol (EtOH) (10 to 300 mM) on the resting pHi and pHi regulators in human aorta smooth muscle cells (HASMCs). Ethanol 111-118 glucose-6-phosphate isomerase Homo sapiens 156-159 26577834-3 2015 The aims of the study were to identify the possible transmembrane pHi regulators and to explore the effects of ethanol (EtOH) (10 to 300 mM) on the resting pHi and pHi regulators in human aorta smooth muscle cells (HASMCs). Ethanol 111-118 glucose-6-phosphate isomerase Homo sapiens 156-159 26577834-3 2015 The aims of the study were to identify the possible transmembrane pHi regulators and to explore the effects of ethanol (EtOH) (10 to 300 mM) on the resting pHi and pHi regulators in human aorta smooth muscle cells (HASMCs). Ethanol 120-124 glucose-6-phosphate isomerase Homo sapiens 156-159 26577834-3 2015 The aims of the study were to identify the possible transmembrane pHi regulators and to explore the effects of ethanol (EtOH) (10 to 300 mM) on the resting pHi and pHi regulators in human aorta smooth muscle cells (HASMCs). Ethanol 120-124 glucose-6-phosphate isomerase Homo sapiens 156-159 26577834-5 2015 The pHi was measured by microspectrofluorimetry with the pH-sensitive dye, BCECF-AM. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 75-83 glucose-6-phosphate isomerase Homo sapiens 4-7 26577834-7 2015 (i) In cultured HASMCs, the resting pHi was 7.19 +- 0.04 and 7.13 +- 0.02 for HEPES- and CO2 /HCO3--buffered solution, respectively. hasmcs 16-22 glucose-6-phosphate isomerase Homo sapiens 36-39 26577834-7 2015 (i) In cultured HASMCs, the resting pHi was 7.19 +- 0.04 and 7.13 +- 0.02 for HEPES- and CO2 /HCO3--buffered solution, respectively. HEPES 78-83 glucose-6-phosphate isomerase Homo sapiens 36-39 26577834-7 2015 (i) In cultured HASMCs, the resting pHi was 7.19 +- 0.04 and 7.13 +- 0.02 for HEPES- and CO2 /HCO3--buffered solution, respectively. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 89-92 glucose-6-phosphate isomerase Homo sapiens 36-39 26577834-7 2015 (i) In cultured HASMCs, the resting pHi was 7.19 +- 0.04 and 7.13 +- 0.02 for HEPES- and CO2 /HCO3--buffered solution, respectively. Bicarbonates 94-98 glucose-6-phosphate isomerase Homo sapiens 36-39 26577834-10 2015 (iv) EtOH induced a biphasic, concentration-dependent change in resting pHi (+0.25 pH unit at 100 mM but only +0.05 pH unit at 300 mM) in bicarbonate-buffered solution, while caused a concentration-dependent decrease in resting pHi (-0.06 pH unit at 300 mM) in HEPES-buffered solution. Ethanol 5-9 glucose-6-phosphate isomerase Homo sapiens 72-75 26577834-10 2015 (iv) EtOH induced a biphasic, concentration-dependent change in resting pHi (+0.25 pH unit at 100 mM but only +0.05 pH unit at 300 mM) in bicarbonate-buffered solution, while caused a concentration-dependent decrease in resting pHi (-0.06 pH unit at 300 mM) in HEPES-buffered solution. Ethanol 5-9 glucose-6-phosphate isomerase Homo sapiens 228-231 26577834-10 2015 (iv) EtOH induced a biphasic, concentration-dependent change in resting pHi (+0.25 pH unit at 100 mM but only +0.05 pH unit at 300 mM) in bicarbonate-buffered solution, while caused a concentration-dependent decrease in resting pHi (-0.06 pH unit at 300 mM) in HEPES-buffered solution. Bicarbonates 138-149 glucose-6-phosphate isomerase Homo sapiens 72-75 26470817-3 2015 Here, we evaluated whether alterations in syncytiotrophoblast pHi could modify water uptake mediated by aquaporins (AQPs) and the contribution of NHEs to this mechanism. Water 79-84 glucose-6-phosphate isomerase Homo sapiens 62-65 26580615-7 2015 Our results showed that EVO inhibited the proliferation of HCT-116 cells, caused accumulation of cells in S and G2/M phases, and reduced the levels of the secreted form of AMF. evodiamine 24-27 glucose-6-phosphate isomerase Homo sapiens 172-175 26580615-9 2015 Taken together, our results suggest that EVO modulates the activity of the p53 signaling pathway to induce apoptosis and downregulate MMP3 expression by inactivating the JAK2/STAT3 pathway through the downregulation of PGI to inhibit migration of HCT-116 human colorectal cancer cells. evodiamine 41-44 glucose-6-phosphate isomerase Homo sapiens 219-222 26002621-16 2015 The positive effects of GPi-DBS on the symptoms of spasmodic dysphonia merits further research as DBS is not commonly applied in this population. dibromsalan 28-31 glucose-6-phosphate isomerase Homo sapiens 24-27 26177601-11 2015 UFH+-GPI significantly increased the odds of MI vs LMWHs, of ST vs UFH+GPI, and of MB vs bivalirudin. Heparin 0-3 glucose-6-phosphate isomerase Homo sapiens 5-8 26177601-11 2015 UFH+-GPI significantly increased the odds of MI vs LMWHs, of ST vs UFH+GPI, and of MB vs bivalirudin. Heparin 0-3 glucose-6-phosphate isomerase Homo sapiens 67-74 26241157-10 2015 Multitarget DBS should be further explored in post-stroke dystonia and may offer improved outcome in other forms of secondary dystonia with limited response to GPi DBS. dibromsalan 12-15 glucose-6-phosphate isomerase Homo sapiens 160-163 26579829-1 2015 Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase, was initially identified as the second glycolytic enzyme that catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. Glucose-6-Phosphate 172-191 glucose-6-phosphate isomerase Homo sapiens 0-29 26579829-1 2015 Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase, was initially identified as the second glycolytic enzyme that catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. Glucose-6-Phosphate 172-191 glucose-6-phosphate isomerase Homo sapiens 31-34 26579829-1 2015 Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase, was initially identified as the second glycolytic enzyme that catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. Glucose-6-Phosphate 172-191 glucose-6-phosphate isomerase Homo sapiens 51-75 26579829-1 2015 Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase, was initially identified as the second glycolytic enzyme that catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. fructose-6-phosphate 195-215 glucose-6-phosphate isomerase Homo sapiens 0-29 26579829-1 2015 Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase, was initially identified as the second glycolytic enzyme that catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. fructose-6-phosphate 195-215 glucose-6-phosphate isomerase Homo sapiens 31-34 26579829-1 2015 Glucose-6-phosphate isomerase (GPI), also known as phosphoglucose isomerase, was initially identified as the second glycolytic enzyme that catalyzes the interconversion of glucose-6-phosphate to fructose-6-phosphate. fructose-6-phosphate 195-215 glucose-6-phosphate isomerase Homo sapiens 51-75 27137142-5 2015 Acetaminophen and indomethacin inhibited PGI-M excretion following single and multiple doses (P = .004 vs placebo). Acetaminophen 0-13 glucose-6-phosphate isomerase Homo sapiens 41-44 27137142-5 2015 Acetaminophen and indomethacin inhibited PGI-M excretion following single and multiple doses (P = .004 vs placebo). Indomethacin 18-30 glucose-6-phosphate isomerase Homo sapiens 41-44 27137142-6 2015 PGI-M excretion inhibition after 1 dose was similar for indomethacin and acetaminophen, but significantly greater with indomethacin after multiple doses (P = .006). Indomethacin 56-68 glucose-6-phosphate isomerase Homo sapiens 0-3 27137142-6 2015 PGI-M excretion inhibition after 1 dose was similar for indomethacin and acetaminophen, but significantly greater with indomethacin after multiple doses (P = .006). Indomethacin 119-131 glucose-6-phosphate isomerase Homo sapiens 0-3 26064449-2 2015 Sodium-hydrogen exchange (NHE) is an important contributor to pHi control in PASMCs. Sodium 0-6 glucose-6-phosphate isomerase Homo sapiens 62-65 26020555-1 2015 Na+/H+ exchangers (NHEs) are the transporter proteins that play an important role in intracellular pH (pHi) regulation, cell differentiation and cell volume and that mediate transepithelial Na+ and HCO3- absorption on the basis of chemical gradients across the plasma membrane. Bicarbonates 198-202 glucose-6-phosphate isomerase Homo sapiens 103-106 26233131-3 2015 It is found that the associated fractionation factor Phi is correlated with the strength of the intramolecular hydrogen bonds. Hydrogen 111-119 glucose-6-phosphate isomerase Homo sapiens 53-56 25711989-4 2015 Oxidant-rich flames (Phi < 1) were characterized by larger particle size, lower particle number concentration, higher black carbon (BC) concentration, lower brown carbon BrC. Carbon 127-133 glucose-6-phosphate isomerase Homo sapiens 21-24 25711989-4 2015 Oxidant-rich flames (Phi < 1) were characterized by larger particle size, lower particle number concentration, higher black carbon (BC) concentration, lower brown carbon BrC. Carbon 166-172 glucose-6-phosphate isomerase Homo sapiens 21-24 25711989-10 2015 The PAH formation was discussed based on the combination of our results and with respect to Phi settings. Polycyclic Aromatic Hydrocarbons 4-7 glucose-6-phosphate isomerase Homo sapiens 92-95 25612232-3 2015 It has been proposed that HCO3(-) is re-captured by the cell to maintain an alkaline pHi . Bicarbonates 26-30 glucose-6-phosphate isomerase Homo sapiens 85-88 25612232-11 2015 Furthermore the Na(+)/HCO3(-) dependent pHi recovery from acidosis was reduced with SLC4A4 knockdown in MDA-MB-231 cells. Bicarbonates 22-26 glucose-6-phosphate isomerase Homo sapiens 40-43 26165085-3 2015 GPI is the glycolipid which anchors 150 kinds of proteins to the plasma membrane. Glycolipids 11-21 glucose-6-phosphate isomerase Homo sapiens 0-3 26064449-2 2015 Sodium-hydrogen exchange (NHE) is an important contributor to pHi control in PASMCs. Hydrogen 7-15 glucose-6-phosphate isomerase Homo sapiens 62-65 26064449-2 2015 Sodium-hydrogen exchange (NHE) is an important contributor to pHi control in PASMCs. pasmcs 77-83 glucose-6-phosphate isomerase Homo sapiens 62-65 29124141-0 2015 A novel binding of GTP stabilizes the structure and modulates the activities of human phosphoglucose isomerase/autocrine motility factor. Guanosine Triphosphate 19-22 glucose-6-phosphate isomerase Homo sapiens 86-110 25861706-8 2015 When the two therapies were combined, GPI-DBS prevented the L-DOPA induced increase in static sway and improved the accuracy of the dynamic task. Levodopa 60-66 glucose-6-phosphate isomerase Homo sapiens 38-41 25861706-9 2015 CONCLUSION: The findings demonstrate GPI-DBS and L-DOPA have differential effects on temporal and spatial aspects of postural control in IPD and that GPI-DBS counteracts some of the adverse effects of L-DOPA. Levodopa 201-207 glucose-6-phosphate isomerase Homo sapiens 150-153 29124141-1 2015 Phosphoglucose isomerase (PGI) catalyzes the interconversion between glucose 6-phosphate and fructose 6-phosphate in the glycolysis pathway. Glucose-6-Phosphate 69-88 glucose-6-phosphate isomerase Homo sapiens 0-24 29124141-1 2015 Phosphoglucose isomerase (PGI) catalyzes the interconversion between glucose 6-phosphate and fructose 6-phosphate in the glycolysis pathway. Glucose-6-Phosphate 69-88 glucose-6-phosphate isomerase Homo sapiens 26-29 29124141-1 2015 Phosphoglucose isomerase (PGI) catalyzes the interconversion between glucose 6-phosphate and fructose 6-phosphate in the glycolysis pathway. fructose-6-phosphate 93-113 glucose-6-phosphate isomerase Homo sapiens 0-24 29124141-1 2015 Phosphoglucose isomerase (PGI) catalyzes the interconversion between glucose 6-phosphate and fructose 6-phosphate in the glycolysis pathway. fructose-6-phosphate 93-113 glucose-6-phosphate isomerase Homo sapiens 26-29 29124141-4 2015 In the present study, a novel interaction between GTP and human PGI was corroborated by UV-induced crosslinking, affinity purification and kinetic study. Guanosine Triphosphate 50-53 glucose-6-phosphate isomerase Homo sapiens 64-67 29124141-7 2015 In addition, GTP stabilizes the structure of human PGI against heat- and detergent-induced denaturation. Guanosine Triphosphate 13-16 glucose-6-phosphate isomerase Homo sapiens 51-54 25890268-7 2015 Intracellular pH (pHi) was measured using fluorescent pH-indicator BCECF-AM. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 67-75 glucose-6-phosphate isomerase Homo sapiens 18-21 25829100-6 2015 The OCDD group performed worse than the OCD group notably on Verbal Fluency Test, PGI memory test, and Object Alternation Test. octachlorodibenzo-4-dioxin 4-8 glucose-6-phosphate isomerase Homo sapiens 82-85 25897971-3 2015 Here we use protein micropatterning combined with single-molecule tracking to put current models to the test: we rearranged lipid-anchored raft proteins (glycosylphosphatidylinositol(GPI)-anchored-mGFP) directly in the live cell plasma membrane and measured the effect on the local membrane environment. Glycosylphosphatidylinositols 154-182 glucose-6-phosphate isomerase Homo sapiens 183-186 25617697-4 2015 In addition, passive fluxes of uncharged species (e.g., CO2, NH3) and charged species (e.g., HCO3(-), [Formula: see text] ) perturb pHi. Bicarbonates 93-97 glucose-6-phosphate isomerase Homo sapiens 132-135 25622724-1 2015 INTRODUCTION: The value of gastric intramucosal pH (pHi) can be calculated from the tonometrically measured partial pressure of carbon dioxide ([Formula: see text]) in the stomach and the arterial bicarbonate content. Carbon Dioxide 128-142 glucose-6-phosphate isomerase Homo sapiens 52-55 25622724-1 2015 INTRODUCTION: The value of gastric intramucosal pH (pHi) can be calculated from the tonometrically measured partial pressure of carbon dioxide ([Formula: see text]) in the stomach and the arterial bicarbonate content. Bicarbonates 197-208 glucose-6-phosphate isomerase Homo sapiens 52-55 26069708-5 2015 Only IL1beta beta decreased pHi in both cell types; all the agents enhanced pHi recovery following an ammonium prepulse in CHC, effect that was attenuated by Na(+)-H(+) exchanger inhibitors, but they had no effect in COC. Ammonium Compounds 102-110 glucose-6-phosphate isomerase Homo sapiens 76-79 27099830-1 2015 BACKGROUND: Dolichyl phosphate-linked mono- and oligosaccharides (DLO) are essential intermediates in protein N-glycosylation, C- and O-mannosylation and GPI anchor biosynthesis. dolichyl phosphate-linked mono- and oligosaccharides 12-64 glucose-6-phosphate isomerase Homo sapiens 127-157 27099830-1 2015 BACKGROUND: Dolichyl phosphate-linked mono- and oligosaccharides (DLO) are essential intermediates in protein N-glycosylation, C- and O-mannosylation and GPI anchor biosynthesis. dlo 66-69 glucose-6-phosphate isomerase Homo sapiens 127-157 27099830-1 2015 BACKGROUND: Dolichyl phosphate-linked mono- and oligosaccharides (DLO) are essential intermediates in protein N-glycosylation, C- and O-mannosylation and GPI anchor biosynthesis. Nitrogen 8-9 glucose-6-phosphate isomerase Homo sapiens 127-157 25802776-6 2015 GPi DBS should be considered in dystonic PKAN patients provided fixed contractures and/or pyramidal symptoms are not present. dibromsalan 4-7 glucose-6-phosphate isomerase Homo sapiens 0-3 25617697-8 2015 We then describe a mathematical model of a spherical cells-which to our knowledge is the first one capable of handling a multitude of buffer reactions-that our team has recently developed to simulate changes in pHi and pHo caused by movements of acid-base equivalents across the plasma membrane of a Xenopus oocyte. acid-base 246-255 glucose-6-phosphate isomerase Homo sapiens 211-214 25219457-5 2014 Bicarbonate stimulates PKA-dependent phosphorylation of two 60kDa proteins identified as Tektin and glucose-6-phosphate isomerase. Bicarbonates 0-11 glucose-6-phosphate isomerase Homo sapiens 100-129 25420189-3 2014 The two sequential aryl C-H activations, assisted by trifluoroacetate as well as the superior leaving group ability of PhI, facilitate the formation of spirocyclic bis-oxindole. spirocyclic bis-oxindole 152-176 glucose-6-phosphate isomerase Homo sapiens 119-122 26152150-7 2015 A recent patent deals with the utilization of cis-Urocanic acid to acidify the pHi and induce apoptosis in tumors. cis-Urocanic acid 46-63 glucose-6-phosphate isomerase Homo sapiens 79-82 24892772-5 2014 Rapid hydrolysis of beta-CM7 in the apical chamber by the Caco-2 cells produced three peptide metabolites: YP, GPI and FPGPI. beta-cm7 20-28 glucose-6-phosphate isomerase Homo sapiens 111-114 25317967-3 2014 We herein report the fabrication of ultrasensitive ratiometric fluorescent pHi imaging probes with robust endosomal escaping capability derived from dual dye-labeled pH-responsive block copolymers, which can directly monitor endosomal escape in living cells and quantitatively measure pHi variations during the entire endocytic and endosomolytic processes. copolymers 186-196 glucose-6-phosphate isomerase Homo sapiens 75-78 25317967-3 2014 We herein report the fabrication of ultrasensitive ratiometric fluorescent pHi imaging probes with robust endosomal escaping capability derived from dual dye-labeled pH-responsive block copolymers, which can directly monitor endosomal escape in living cells and quantitatively measure pHi variations during the entire endocytic and endosomolytic processes. copolymers 186-196 glucose-6-phosphate isomerase Homo sapiens 285-288 25262930-4 2014 Here, we present methodology that permits identification of GPI-APs liberated directly from the surface of intact mammalian cells through exploitation of their appended glycans to enrich for these proteins ahead of LC-MS/MS analyses. Polysaccharides 169-176 glucose-6-phosphate isomerase Homo sapiens 60-63 25241983-5 2014 We therefore investigated the mechanism of pHi recovery from intracellular acidosis and alkalosis, induced by NH4Cl-prepulse and Na-acetate-prepulse, respectively, using intracellular 2",7"-bis(2-carboxethyl)-5(6)- carboxy-fluorescein (BCECF)-fluorescence in HRASMCs. Ammonium Chloride 110-115 glucose-6-phosphate isomerase Homo sapiens 43-46 25241983-5 2014 We therefore investigated the mechanism of pHi recovery from intracellular acidosis and alkalosis, induced by NH4Cl-prepulse and Na-acetate-prepulse, respectively, using intracellular 2",7"-bis(2-carboxethyl)-5(6)- carboxy-fluorescein (BCECF)-fluorescence in HRASMCs. na-acetate 129-139 glucose-6-phosphate isomerase Homo sapiens 43-46 25241983-5 2014 We therefore investigated the mechanism of pHi recovery from intracellular acidosis and alkalosis, induced by NH4Cl-prepulse and Na-acetate-prepulse, respectively, using intracellular 2",7"-bis(2-carboxethyl)-5(6)- carboxy-fluorescein (BCECF)-fluorescence in HRASMCs. bcecf 236-241 glucose-6-phosphate isomerase Homo sapiens 43-46 25241983-7 2014 The resting pHi is 7.22 +- 0.03 and 7.17 +- 0.02 for HEPES- (nominally HCO3--free) and CO2/HCO3-- buffered solution, respectively. HEPES 53-58 glucose-6-phosphate isomerase Homo sapiens 12-15 25241983-5 2014 We therefore investigated the mechanism of pHi recovery from intracellular acidosis and alkalosis, induced by NH4Cl-prepulse and Na-acetate-prepulse, respectively, using intracellular 2",7"-bis(2-carboxethyl)-5(6)- carboxy-fluorescein (BCECF)-fluorescence in HRASMCs. 2",7"-bis(2-carboxethyl)-5(6)- carboxy-fluorescein 184-234 glucose-6-phosphate isomerase Homo sapiens 43-46 25241983-7 2014 The resting pHi is 7.22 +- 0.03 and 7.17 +- 0.02 for HEPES- (nominally HCO3--free) and CO2/HCO3-- buffered solution, respectively. Bicarbonates 71-75 glucose-6-phosphate isomerase Homo sapiens 12-15 25241983-7 2014 The resting pHi is 7.22 +- 0.03 and 7.17 +- 0.02 for HEPES- (nominally HCO3--free) and CO2/HCO3-- buffered solution, respectively. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 87-90 glucose-6-phosphate isomerase Homo sapiens 12-15 24992467-4 2014 DFT calculations of the stable tautomer of selected (Ph)ImC analogues suggested a relationship between the HOMO-LUMO gap and Phi and explained the loss of fluorescence in the nitro analogue. nitro 175-180 glucose-6-phosphate isomerase Homo sapiens 125-128 25241983-7 2014 The resting pHi is 7.22 +- 0.03 and 7.17 +- 0.02 for HEPES- (nominally HCO3--free) and CO2/HCO3-- buffered solution, respectively. Bicarbonates 91-95 glucose-6-phosphate isomerase Homo sapiens 12-15 25241983-8 2014 In HEPES-buffered solution, a pHi recovery from induced intracellular acidosis could be blocked completely by 30 muM HOE 694 (3-methylsulfonyl-4-piperidinobenzoyl, guanidine hydrochloride) a specific NHE inhibitor, or by removing [Na+]0. HEPES 3-8 glucose-6-phosphate isomerase Homo sapiens 30-33 25241983-8 2014 In HEPES-buffered solution, a pHi recovery from induced intracellular acidosis could be blocked completely by 30 muM HOE 694 (3-methylsulfonyl-4-piperidinobenzoyl, guanidine hydrochloride) a specific NHE inhibitor, or by removing [Na+]0. 3-methylsulfonyl-4-piperidinobenzoyl guanidine 117-124 glucose-6-phosphate isomerase Homo sapiens 30-33 25241983-8 2014 In HEPES-buffered solution, a pHi recovery from induced intracellular acidosis could be blocked completely by 30 muM HOE 694 (3-methylsulfonyl-4-piperidinobenzoyl, guanidine hydrochloride) a specific NHE inhibitor, or by removing [Na+]0. 3-methylsulfonyl-4-piperidinobenzoyl 126-162 glucose-6-phosphate isomerase Homo sapiens 30-33 25241983-8 2014 In HEPES-buffered solution, a pHi recovery from induced intracellular acidosis could be blocked completely by 30 muM HOE 694 (3-methylsulfonyl-4-piperidinobenzoyl, guanidine hydrochloride) a specific NHE inhibitor, or by removing [Na+]0. Guanidine 164-187 glucose-6-phosphate isomerase Homo sapiens 30-33 25241983-10 2014 Moreover, alpha-cyano-4-hydroxycinnamate (CHC; a specific blocker of MCT) blocked the lactate-induced pHi changes. alpha-cyano-4-hydroxycinnamate 10-40 glucose-6-phosphate isomerase Homo sapiens 102-105 25241983-10 2014 Moreover, alpha-cyano-4-hydroxycinnamate (CHC; a specific blocker of MCT) blocked the lactate-induced pHi changes. Lactic Acid 86-93 glucose-6-phosphate isomerase Homo sapiens 102-105 25042711-7 2014 His-tag-containing recombinant human glucose-6-phosphate isomerase in combination with an anti-His-tag monoclonal antibody was instrumental to develop the method. Histidine 0-3 glucose-6-phosphate isomerase Homo sapiens 37-66 24970589-6 2014 ELISA tests demonstrated that P.rbeta2-GPI DV interacted with oxLDL via 7-ketocholesteryl-9-carboxynonanoate (oxLig-1), a negatively charged lipid moiety of oxLDL. 7-ketocholesteryl-9-carboxynonanoate 72-108 glucose-6-phosphate isomerase Homo sapiens 39-42 25116406-3 2014 Furthermore, it can effectively separate these monohalobenzenes and exhibits a clear affinity for monohalobenzenes according to the following order: PhI > PhBr > PhCl > PhF. monohalobenzenes 47-63 glucose-6-phosphate isomerase Homo sapiens 149-152 25116406-3 2014 Furthermore, it can effectively separate these monohalobenzenes and exhibits a clear affinity for monohalobenzenes according to the following order: PhI > PhBr > PhCl > PhF. monohalobenzenes 98-114 glucose-6-phosphate isomerase Homo sapiens 149-152 24827113-0 2014 Utilizing hydrogen sulfide as a novel anti-cancer agent by targeting cancer glycolysis and pH imbalance. Hydrogen Sulfide 10-26 glucose-6-phosphate isomerase Homo sapiens 91-93 24827113-11 2014 CONCLUSIONS AND IMPLICATIONS: Low and continuous exposure to H2 S targets metabolic processes and pH homeostasis in cancer cells, potentially serving as a novel and selective anti-cancer strategy. Hydrogen 61-63 glucose-6-phosphate isomerase Homo sapiens 98-100 23934106-4 2014 We previously described generation of a recombinant fusion protein linking TIMP-1 to glycosylphophatidylinositol (GPI) anchor (TIMP-1-GPI) that efficiently directs the inhibitor to cell surfaces. glycosylphophatidylinositol 85-112 glucose-6-phosphate isomerase Homo sapiens 114-117 24576828-6 2014 Conversely, exogenous AMF overcame the inhibitory effect of EGF receptor inhibitor gefitinib on invasive motility by activating HER2 signaling. Gefitinib 83-92 glucose-6-phosphate isomerase Homo sapiens 22-25 24904309-4 2014 Systemic administration of L-DOPA alleviated parkinsonian motor signs and decreased abnormal neuronal oscillations (8-15 Hz) in the internal (GPi) and external (GPe) segments of the globus pallidus and the subthalamic nucleus (STN). Levodopa 27-33 glucose-6-phosphate isomerase Homo sapiens 142-145 24904309-5 2014 Inactivation of the STN by muscimol (GABAA receptor agonist) injection also ameliorated parkinsonian signs and suppressed GPi oscillations. Muscimol 27-35 glucose-6-phosphate isomerase Homo sapiens 122-125 24607544-5 2014 In prostate cancer cells cytosolic pH (pHi) was determined utilizing 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein-fluorescence and NHE-activity utilizing Na(+)-dependent cytosolic realkalinization following an ammonium pulse. 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein 69-124 glucose-6-phosphate isomerase Homo sapiens 39-42 24607544-6 2014 TAC (100 nM) significantly increased pHi and NHE-activity, effects abrogated by NHE1-inhibitor cariporide (10 muM), SGK1-inhibitors EMD638683 (50 muM) and GSK650349 (10 muM) and ROCK-inhibitors Y-27632 (10 muM) and fasudil (100 muM). cariporide 95-105 glucose-6-phosphate isomerase Homo sapiens 37-40 24714927-5 2014 Due to the strong H-bond network formed by the head groups, GPI-fragment both segregates and induces order into a model membrane phospholipid (POPC) that mimics the liquid-disordered phase of cell membranes. Phospholipids 129-141 glucose-6-phosphate isomerase Homo sapiens 60-63 24292536-8 2014 These results indicate that ionomycin may strongly inhibit human acute T lymphocyte leukemia progress in the presence of PDBu through the inhibition of ERK1/2 signaling, the activation of caspase-3 and the attenuation of TGF-beta mediated by the [Ca2(+)]i and pHi enhancement, providing a novel insight into function and potential application of both ionomycin and PDBu. Ionomycin 28-37 glucose-6-phosphate isomerase Homo sapiens 260-263 24608976-10 2014 These observations provided novel information about the pathogenetic role of a TESC-NHE1-pHi axis in mediating sorafenib resistance in AML. Sorafenib 111-120 glucose-6-phosphate isomerase Homo sapiens 89-92 24295212-3 2014 Intracellular pH (pHi) was determined using 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) and whole spectrum analysis. 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein 44-99 glucose-6-phosphate isomerase Homo sapiens 18-21 24753757-3 2014 Gastric mucosal perfusion and tissue oxygenation were monitored by measuring the gastric mucosal pH (pHi), which was determined using a carbon dioxide tonometer inserted through a nasogastric tube. Carbon Dioxide 136-150 glucose-6-phosphate isomerase Homo sapiens 101-104 24753757-6 2014 The pHi of the HES 130/0.4 group was significantly higher than that of the HES 200/0.5 group two hours after skin incision and at the end of surgery (P<0.05). Hydroxyethyl Starch Derivatives 15-18 glucose-6-phosphate isomerase Homo sapiens 4-7 24587308-6 2014 The mechanism of pHi recovery from intracellular acidosis (induced by NH4Cl-prepulse) is determined using BCECF-fluorescence in cultured HRASMCs. Ammonium Chloride 70-75 glucose-6-phosphate isomerase Homo sapiens 17-20 24587308-6 2014 The mechanism of pHi recovery from intracellular acidosis (induced by NH4Cl-prepulse) is determined using BCECF-fluorescence in cultured HRASMCs. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 106-111 glucose-6-phosphate isomerase Homo sapiens 17-20 24184393-8 2014 Specially, total dietary fiber amount and IDF ratio are more effective than SDF ratio to lower the pGI value. [(R)-(2,4-dichlorophenyl)(sulfanyl)methyl]phosphonic acid 76-79 glucose-6-phosphate isomerase Homo sapiens 99-102 24918078-5 2014 The effect of curcumin on intracellular pH (pHi) was measured by the fluorescent pH indicator 2,7-bicarboxyethyl-5,6-carboxyfluorescein-acetoxymethyl ester (BCECF-AM). Curcumin 14-22 glucose-6-phosphate isomerase Homo sapiens 44-47 24918078-5 2014 The effect of curcumin on intracellular pH (pHi) was measured by the fluorescent pH indicator 2,7-bicarboxyethyl-5,6-carboxyfluorescein-acetoxymethyl ester (BCECF-AM). 2,7-bicarboxyethyl-5,6-carboxyfluorescein-acetoxymethyl ester 94-155 glucose-6-phosphate isomerase Homo sapiens 44-47 24918078-5 2014 The effect of curcumin on intracellular pH (pHi) was measured by the fluorescent pH indicator 2,7-bicarboxyethyl-5,6-carboxyfluorescein-acetoxymethyl ester (BCECF-AM). bcecf-am 157-165 glucose-6-phosphate isomerase Homo sapiens 44-47 24918078-11 2014 This is the first study to our knowledge which examined the effect of curcumin on sperm pHi and membrane polarization that affect sperm forward motility. Curcumin 70-78 glucose-6-phosphate isomerase Homo sapiens 88-91 24446917-1 2014 The folate receptor (FR) is a GPI anchored cell surface glycoprotein that functions to facilitate folic acid uptake and mediate signal transduction. Folic Acid 98-108 glucose-6-phosphate isomerase Homo sapiens 30-33 24295212-3 2014 Intracellular pH (pHi) was determined using 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) and whole spectrum analysis. bcecf 101-106 glucose-6-phosphate isomerase Homo sapiens 18-21 24295212-6 2014 RESULTS: Treatment with LND at pHe 6.7 reduced pHi to 6.30 +- 0.21, reduced thermal induction of HSPs, and sensitised cells growing at pHe 6.7 to 42 C. When LND was combined with an acute acidification from pHe 6.7 to pHe 6.5, pHi was reduced to 6.09 +- 0.26, and additional sensitisation was observed. Phenylalanine 31-34 glucose-6-phosphate isomerase Homo sapiens 47-50 24295212-8 2014 CONCLUSIONS: The results show that LND can reduce pHi in human melanoma cells cultured at a tumour-like low pHe so that the 42 C induction of HSPs are abrogated and the cells are sensitised to thermal therapy. Phenylalanine 108-111 glucose-6-phosphate isomerase Homo sapiens 50-53 24738141-1 2014 31P magnetic resonance spectroscopy (31P MRS) can measure intracellular pH (pHi) using the chemical shift difference between pH-dependent inorganic phosphate (Pi) and a pH-independent reference peak. Phosphates 138-157 glucose-6-phosphate isomerase Homo sapiens 76-79 25349604-0 2014 Efficacy of Optical Internal Urethrotomy and Intralesional Injection of Vatsala-Santosh PGI Tri-Inject (Triamcinolone, Mitomycin C, and Hyaluronidase) in the Treatment of Anterior Urethral Stricture. Triamcinolone 104-117 glucose-6-phosphate isomerase Homo sapiens 88-91 25349604-0 2014 Efficacy of Optical Internal Urethrotomy and Intralesional Injection of Vatsala-Santosh PGI Tri-Inject (Triamcinolone, Mitomycin C, and Hyaluronidase) in the Treatment of Anterior Urethral Stricture. Mitomycin 119-130 glucose-6-phosphate isomerase Homo sapiens 88-91 25349604-2 2014 To study the efficacy of optical internal urethrotomy with intralesional injection of Vatsala-Santosh PGI tri-inject (triamcinolone, mitomycin C, and hyaluronidase) in the treatment of anterior urethral stricture. Triamcinolone 118-131 glucose-6-phosphate isomerase Homo sapiens 102-105 25349604-13 2014 Optical internal urethrotomy with intralesional injection of Vatsala-Santosh PGI tri-inject (triamcinolone, mitomycin C, and hyaluronidase) is a safe and effective minimally invasive therapeutic modality for short segment anterior urethral strictures. Triamcinolone 93-106 glucose-6-phosphate isomerase Homo sapiens 77-80 25349604-13 2014 Optical internal urethrotomy with intralesional injection of Vatsala-Santosh PGI tri-inject (triamcinolone, mitomycin C, and hyaluronidase) is a safe and effective minimally invasive therapeutic modality for short segment anterior urethral strictures. Mitomycin 108-119 glucose-6-phosphate isomerase Homo sapiens 77-80 24481225-11 2014 RESULTS: The pHi of hUC-MSCs was decreased upon cariporide treatment. cariporide 48-58 glucose-6-phosphate isomerase Homo sapiens 13-16 24606425-5 2014 The effect of PGI/ AMF on proliferation was measured by typan blue assay and antibody array. typan blue 56-66 glucose-6-phosphate isomerase Homo sapiens 14-17 24481225-14 2014 CONCLUSION: Decreased pHi induced by cariporide differentially contributes to hUC-MSCs differentiation. cariporide 37-47 glucose-6-phosphate isomerase Homo sapiens 22-25 24393154-3 2014 We recently described the effect of a glycosylphosphatidylinositol (GPI) anchored version of TIMP-1 on dermal fibroblast biology. Glycosylphosphatidylinositols 38-66 glucose-6-phosphate isomerase Homo sapiens 68-71 25322912-4 2014 BCECF microfluorimetry was used to assess changes of intracellular pH (pHi) after acute intracellular acid load (NH4Cl prepulsing). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 0-5 glucose-6-phosphate isomerase Homo sapiens 71-74 25322912-4 2014 BCECF microfluorimetry was used to assess changes of intracellular pH (pHi) after acute intracellular acid load (NH4Cl prepulsing). Ammonium Chloride 113-118 glucose-6-phosphate isomerase Homo sapiens 71-74 25322912-10 2014 The H(+)/K(+) ATPase inhibitor SCH28080 (100 microM) diminishes K(+)-dependent pHi recovery after intracellular acid load and blocks RVI after osmotic cell shrinkage. Sch 28080 31-39 glucose-6-phosphate isomerase Homo sapiens 79-82 24714077-1 2014 BACKGROUND: Bicarbonate transport has crucial roles in regulating intracellular pH (pHi) in a variety of cells. Bicarbonates 12-23 glucose-6-phosphate isomerase Homo sapiens 84-87 24714077-5 2014 Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na(+)/HCO3(-) cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. Ammonium Compounds 28-36 glucose-6-phosphate isomerase Homo sapiens 83-86 24714077-5 2014 Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na(+)/HCO3(-) cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. S 0859 compound 215-220 glucose-6-phosphate isomerase Homo sapiens 83-86 24714077-5 2014 Neutrophils acidified by an ammonium prepulse showed an EIPA-resistant recovery of pHi that was inhibited by the blocker of the anionic transporters SITS or the Na(+)/HCO3(-) cotransporter (NBC) selective inhibitor S0859, and abolished when sodium was removed from the extracellular medium. Sodium 241-247 glucose-6-phosphate isomerase Homo sapiens 83-86 24068510-5 2013 The significant risk factors for Gp I were infrequent consumption of green vegetable (odds ratio (OR), 2.3; p < 0.05), intake of tea/coffee (OR 3.3; p < 0.01) and less consumption of sugar (p < 0.01). Sugars 189-194 glucose-6-phosphate isomerase Homo sapiens 33-37 24381558-7 2013 Major mechanisms for maintenance of pHi homeostasis include monocarboxylate, bicarbonate, and proton transporters. monocarboxylate 60-75 glucose-6-phosphate isomerase Homo sapiens 36-39 24381558-7 2013 Major mechanisms for maintenance of pHi homeostasis include monocarboxylate, bicarbonate, and proton transporters. Bicarbonates 77-88 glucose-6-phosphate isomerase Homo sapiens 36-39 24128440-3 2013 This account has reviewed the recent progress made in disclosing GPI and GPI-anchored protein biosynthesis, in the chemical and chemoenzymatic synthesis of GPIs and GPI-anchored proteins, and in understanding the conformation, organization, and distribution of GPIs in the lipid membrane. Glycosylphosphatidylinositols 156-160 glucose-6-phosphate isomerase Homo sapiens 65-68 24128440-3 2013 This account has reviewed the recent progress made in disclosing GPI and GPI-anchored protein biosynthesis, in the chemical and chemoenzymatic synthesis of GPIs and GPI-anchored proteins, and in understanding the conformation, organization, and distribution of GPIs in the lipid membrane. Glycosylphosphatidylinositols 156-160 glucose-6-phosphate isomerase Homo sapiens 73-76 24128440-3 2013 This account has reviewed the recent progress made in disclosing GPI and GPI-anchored protein biosynthesis, in the chemical and chemoenzymatic synthesis of GPIs and GPI-anchored proteins, and in understanding the conformation, organization, and distribution of GPIs in the lipid membrane. Glycosylphosphatidylinositols 261-265 glucose-6-phosphate isomerase Homo sapiens 65-68 24128440-3 2013 This account has reviewed the recent progress made in disclosing GPI and GPI-anchored protein biosynthesis, in the chemical and chemoenzymatic synthesis of GPIs and GPI-anchored proteins, and in understanding the conformation, organization, and distribution of GPIs in the lipid membrane. Glycosylphosphatidylinositols 261-265 glucose-6-phosphate isomerase Homo sapiens 73-76 24005470-3 2013 After acid loading, in the presence of HCO3(-), ~50% of the pHi recovery phase was blocked by the Na(+)/H(+) exchanger inhibitors EIPA (10-50 muM) and amiloride (1 mM) and was fully cancelled by 30 muM EIPA under nominally HCO3(-)-free conditions. Bicarbonates 39-43 glucose-6-phosphate isomerase Homo sapiens 60-63 24005470-3 2013 After acid loading, in the presence of HCO3(-), ~50% of the pHi recovery phase was blocked by the Na(+)/H(+) exchanger inhibitors EIPA (10-50 muM) and amiloride (1 mM) and was fully cancelled by 30 muM EIPA under nominally HCO3(-)-free conditions. Amiloride 151-160 glucose-6-phosphate isomerase Homo sapiens 60-63 24005470-3 2013 After acid loading, in the presence of HCO3(-), ~50% of the pHi recovery phase was blocked by the Na(+)/H(+) exchanger inhibitors EIPA (10-50 muM) and amiloride (1 mM) and was fully cancelled by 30 muM EIPA under nominally HCO3(-)-free conditions. Bicarbonates 223-227 glucose-6-phosphate isomerase Homo sapiens 60-63 24005470-4 2013 In addition, in the presence of HCO3(-), pHi recovery after acid loading was completely blocked when Na(+) was omitted in the buffer. Bicarbonates 32-36 glucose-6-phosphate isomerase Homo sapiens 41-44 24005470-5 2013 pHi recovery after acidification in HEK cells was repeated in the presence of the NBC inhibitor S0859, and the pHi recovery was inhibited by S0859 in a dose-dependent manner (Ki = 30 muM, full inhibition at 60 muM), which confirmed NBC Na(+)/HCO3(-) cotransporter activation. Bicarbonates 242-246 glucose-6-phosphate isomerase Homo sapiens 0-3 24005470-5 2013 pHi recovery after acidification in HEK cells was repeated in the presence of the NBC inhibitor S0859, and the pHi recovery was inhibited by S0859 in a dose-dependent manner (Ki = 30 muM, full inhibition at 60 muM), which confirmed NBC Na(+)/HCO3(-) cotransporter activation. Bicarbonates 242-246 glucose-6-phosphate isomerase Homo sapiens 111-114 24005470-7 2013 Finally, exposure of HEK cells to high CO2 significantly increased the HCO3(-)-dependent recovery of pHi after acid loading. Carbon Dioxide 39-42 glucose-6-phosphate isomerase Homo sapiens 101-104 24005470-7 2013 Finally, exposure of HEK cells to high CO2 significantly increased the HCO3(-)-dependent recovery of pHi after acid loading. Bicarbonates 71-75 glucose-6-phosphate isomerase Homo sapiens 101-104 24224877-5 2013 The corresponding thulium complex exhibits emission efficiencies (quantum yield Phi > 0.12%; lifetime tauobs = 4.6 mus; brightness epsilonPhi > 30 M(-1) cm(-1)) and can even be detected at low micromolar concentrations in high-phonon solvents like water without the need for laser excitation. Thulium 18-25 glucose-6-phosphate isomerase Homo sapiens 80-83 23380706-2 2013 These events include structural remodeling of both the lipid and glycan moieties of GPI, recruitment of GPI-APs into ER exit sites, association with the cargo receptor, p24 protein complex, and packaging into COPII coated transport vesicles. Polysaccharides 65-71 glucose-6-phosphate isomerase Homo sapiens 84-87 24073915-2 2013 Meanwhile, replacement of sodium ion with an ammonium amphiphile 1 gives a lipid complex 1[Eu(nta)4] which shows distinct changes: its luminescence quantum yield Phi is remarkably increased to ~0.6 above the water content of ~60 vol. Sodium 26-32 glucose-6-phosphate isomerase Homo sapiens 162-165 24073915-2 2013 Meanwhile, replacement of sodium ion with an ammonium amphiphile 1 gives a lipid complex 1[Eu(nta)4] which shows distinct changes: its luminescence quantum yield Phi is remarkably increased to ~0.6 above the water content of ~60 vol. Water 208-213 glucose-6-phosphate isomerase Homo sapiens 162-165 22549898-4 2013 Only UA efficiently suppressed the AMF/PGI-induced Huh7 cell migration and MMP-3 secretion. ursolic acid 5-7 glucose-6-phosphate isomerase Homo sapiens 39-42 22549898-5 2013 Additionally, UA inhibited the AMF/PGI-mediated protection against TGF-beta-induced apoptosis in Hep3B cells, whereas OA, tangeretin, and nobiletin had no effect. ursolic acid 14-16 glucose-6-phosphate isomerase Homo sapiens 35-38 22549898-6 2013 In Huh7 cells and tumor tissues, UA disrupted the Src/RhoA/PI 3-kinase signaling and complex formation induced by AMF/PGI. ursolic acid 33-35 glucose-6-phosphate isomerase Homo sapiens 118-121 22549898-7 2013 In the Hep3B system, UA dramatically suppressed AMF/PGI-induced anti-apoptotic signaling transmission, including Akt, p85, Bad, and Stat3 phosphorylation. ursolic acid 21-23 glucose-6-phosphate isomerase Homo sapiens 52-55 24046745-12 2013 Furthermore, these results suggest that the mechanisms of GPi DBS in dystonia may involve improvement of TC relay fidelity. Technetium 105-107 glucose-6-phosphate isomerase Homo sapiens 58-61 24043698-6 2013 http://dx.doi.org/10.1083/jcb.201308034) show that pHi increase activates FAK by causing deprotonation of histidine 58 in its FERM (band 4.1, ezrin, radixin, moesin) homology domain, which exposes a region important for FAK autophosphorylation. Histidine 106-115 glucose-6-phosphate isomerase Homo sapiens 51-54 23677800-7 2013 In BCECF-loaded NPE, DIDS was found to reduce cytoplasmic pH (pHi). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 21-25 glucose-6-phosphate isomerase Homo sapiens 62-65 23677800-8 2013 PP2-sensitive Na-K-ATPase activity inhibition, (86)Rb uptake suppression, and SFK activation were observed when a similar reduction of pHi was imposed by low-pH medium or an ammonium chloride withdrawal maneuver. Rubidium 51-53 glucose-6-phosphate isomerase Homo sapiens 135-138 23677800-8 2013 PP2-sensitive Na-K-ATPase activity inhibition, (86)Rb uptake suppression, and SFK activation were observed when a similar reduction of pHi was imposed by low-pH medium or an ammonium chloride withdrawal maneuver. Ammonium Chloride 174-191 glucose-6-phosphate isomerase Homo sapiens 135-138 23677800-9 2013 PP2 and the ERK inhibitor U0126 prevented robust ERK1/2 activation in cells exposed to DIDS or subjected to pHi reduction, but U0126 did not prevent SFK activation or the Na-K-ATPase activity response. U 0126 26-31 glucose-6-phosphate isomerase Homo sapiens 108-111 23611688-12 2013 We propose that the patients with DT with a mild dystonia should be considered for Vim DBS procedure and the coexistence of severe DT and dystonia may be successfully controlled by combined GPi and Vim DBS surgeries. Thymidine 131-133 glucose-6-phosphate isomerase Homo sapiens 190-193 23397991-2 2013 To uncover novel roles and participants of the pHi regulatory system, we have screened an Arabidopsis mutant collection for resistance of seed germination to intracellular acidification induced by weak organic acids (acetic, propionic, sorbic). organic acids 202-215 glucose-6-phosphate isomerase Homo sapiens 47-50 23611688-8 2013 GPi was targeted in four patients with bilateral DBS procedure in every patient from this subgroup. dibromsalan 49-52 glucose-6-phosphate isomerase Homo sapiens 0-3 23611688-11 2013 Patients with GPi DBS had average DT improvement by approximately 50% but their dystonia symptoms were markedly improved. dibromsalan 18-21 glucose-6-phosphate isomerase Homo sapiens 14-17 23611688-11 2013 Patients with GPi DBS had average DT improvement by approximately 50% but their dystonia symptoms were markedly improved. Thymidine 34-36 glucose-6-phosphate isomerase Homo sapiens 14-17 23585132-10 2013 By altering the trajectory of the AP repolarization, low pHi has a significant indirect effect on calcium handling, especially evident in epicardial cells. Calcium 98-105 glucose-6-phosphate isomerase Homo sapiens 57-60 23656410-0 2013 One-pot synthesis of 3-hydroxyquinolin-2(1H)-ones from N-phenylacetoacetamide via PhI(OCOCF3)2-mediated alpha-hydroxylation and H2SO4-promoted intramolecular cyclization. 3-hydroxyquinolin-2(1H)-one 21-49 glucose-6-phosphate isomerase Homo sapiens 82-92 23656410-0 2013 One-pot synthesis of 3-hydroxyquinolin-2(1H)-ones from N-phenylacetoacetamide via PhI(OCOCF3)2-mediated alpha-hydroxylation and H2SO4-promoted intramolecular cyclization. N-phenylacetoacetamide 55-77 glucose-6-phosphate isomerase Homo sapiens 82-92 23656410-1 2013 A clean, one-pot synthesis of the biologically important 3-hydroxyquinolin-2(1H)-one compounds has been realized from the readily available N-phenylacetoacetamide derivatives through a PhI(OCOCF3)2-mediated alpha-hydroxylation and a H2SO4-promoted intramolecular condensation. 3-hydroxyquinolin-2(1H)-one 57-84 glucose-6-phosphate isomerase Homo sapiens 185-195 23656410-1 2013 A clean, one-pot synthesis of the biologically important 3-hydroxyquinolin-2(1H)-one compounds has been realized from the readily available N-phenylacetoacetamide derivatives through a PhI(OCOCF3)2-mediated alpha-hydroxylation and a H2SO4-promoted intramolecular condensation. N-phenylacetoacetamide 140-162 glucose-6-phosphate isomerase Homo sapiens 185-195 23656410-1 2013 A clean, one-pot synthesis of the biologically important 3-hydroxyquinolin-2(1H)-one compounds has been realized from the readily available N-phenylacetoacetamide derivatives through a PhI(OCOCF3)2-mediated alpha-hydroxylation and a H2SO4-promoted intramolecular condensation. sulfuric acid 233-238 glucose-6-phosphate isomerase Homo sapiens 185-195 23597945-2 2013 In this presentation, we will highlight some of the HIF-1-induced gene products - carbonic anhydrases IX and XII (CAs) and monocarboxylate transporters (MCTs) - which regulate the pHi by controlling export of metabolically-generated acids (carbonic and lactic acids). carbonic and 240-252 glucose-6-phosphate isomerase Homo sapiens 180-183 23597945-2 2013 In this presentation, we will highlight some of the HIF-1-induced gene products - carbonic anhydrases IX and XII (CAs) and monocarboxylate transporters (MCTs) - which regulate the pHi by controlling export of metabolically-generated acids (carbonic and lactic acids). Lactic Acid 253-265 glucose-6-phosphate isomerase Homo sapiens 180-183 23597945-3 2013 We reported that targeting these pHi-regulated processes through inhibition of either HIF-1-induced CAIX/CAXII or HIF-1-induced MCT4, MCT1 or Basigin/EMMPRIN/CD147 chaperone of MCTs, severely restricts glycolysis-generated ATP levels and tumor growth. Adenosine Triphosphate 223-226 glucose-6-phosphate isomerase Homo sapiens 33-36 23094951-2 2013 MATERIALS AND METHODS: We recorded local field potentials from the DBS lead in the GPi of four patients (seven hemispheres) with secondary dystonia and from one patient (two hemispheres) with primary DYT3+ dystonia. dibromsalan 67-70 glucose-6-phosphate isomerase Homo sapiens 83-86 23397991-4 2013 During acetic acid treatment the root epidermal cells of the mutant maintain a higher pHi and a more depolarized plasma membrane electrical potential than wild-type cells. Acetic Acid 7-18 glucose-6-phosphate isomerase Homo sapiens 86-89 23201122-3 2013 As pH(i) decreases, histones are globally deacetylated by histone deacetylases (HDACs), and the released acetate anions are coexported with protons out of the cell by monocarboxylate transporters (MCTs), preventing further reductions in pH(i). Acetates 105-112 glucose-6-phosphate isomerase Homo sapiens 3-8 23883461-2 2013 OBJECTIVE: to determine the prevalence of impaired fasting glucose (IFG) and postprandial glucose intolerance (PGI) in individuals with diabetic parent and risk factors for developing DM2. 2cer 111-114 glucose-6-phosphate isomerase Homo sapiens 77-109 23364618-1 2013 Fluorescent nucleosides (dU(bmz)) with desirable fluorescence quantum yield (Phi) are synthesized from almost non-fluorescent 5-fdU and o-phenylenediamine derivatives. Nucleosides 12-23 glucose-6-phosphate isomerase Homo sapiens 77-80 23364618-1 2013 Fluorescent nucleosides (dU(bmz)) with desirable fluorescence quantum yield (Phi) are synthesized from almost non-fluorescent 5-fdU and o-phenylenediamine derivatives. du 25-27 glucose-6-phosphate isomerase Homo sapiens 77-80 23364618-1 2013 Fluorescent nucleosides (dU(bmz)) with desirable fluorescence quantum yield (Phi) are synthesized from almost non-fluorescent 5-fdU and o-phenylenediamine derivatives. 2-(2-hydroxy-phenyl)-1h-benzoimidazole-5-carboxamidine 28-31 glucose-6-phosphate isomerase Homo sapiens 77-80 23364618-1 2013 Fluorescent nucleosides (dU(bmz)) with desirable fluorescence quantum yield (Phi) are synthesized from almost non-fluorescent 5-fdU and o-phenylenediamine derivatives. 5-formyl-2'-deoxyuridine 126-131 glucose-6-phosphate isomerase Homo sapiens 77-80 23364618-1 2013 Fluorescent nucleosides (dU(bmz)) with desirable fluorescence quantum yield (Phi) are synthesized from almost non-fluorescent 5-fdU and o-phenylenediamine derivatives. 1,2-diaminobenzene 136-154 glucose-6-phosphate isomerase Homo sapiens 77-80 23360219-4 2013 The spectral and photophysical properties (taus, Phif, Phi(ic), Phi(isc), tauT, k(f), k(ic), k(isc)) of the bacteriochlorins have been characterized. bacteriochlorin 108-124 glucose-6-phosphate isomerase Homo sapiens 49-52 23360219-4 2013 The spectral and photophysical properties (taus, Phif, Phi(ic), Phi(isc), tauT, k(f), k(ic), k(isc)) of the bacteriochlorins have been characterized. bacteriochlorin 108-124 glucose-6-phosphate isomerase Homo sapiens 55-58 23480184-2 2013 Nine GPI arginine-bearing peptides in human GPI protein were selected and cyclic citrullinated GPI peptides (CCG-1-9) were constructed. Peptides 26-34 glucose-6-phosphate isomerase Homo sapiens 5-8 23480184-2 2013 Nine GPI arginine-bearing peptides in human GPI protein were selected and cyclic citrullinated GPI peptides (CCG-1-9) were constructed. Peptides 26-34 glucose-6-phosphate isomerase Homo sapiens 44-47 23480184-2 2013 Nine GPI arginine-bearing peptides in human GPI protein were selected and cyclic citrullinated GPI peptides (CCG-1-9) were constructed. Peptides 26-34 glucose-6-phosphate isomerase Homo sapiens 44-47 23096710-4 2013 Proteins anchored by glycosylphosphatidylinositol (GPI), when purified and added to cells or tissues, are efficiently incorporated into their surface membranes. Glycosylphosphatidylinositols 21-49 glucose-6-phosphate isomerase Homo sapiens 51-54 23272736-5 2013 These particles exhibited a bright fluorescence emission with quantum yields as high as Phi = 84%, which could optionally be tuned to longer wavelengths by energy transfer to the perylene monoimide dye. perylenemonoimide 179-197 glucose-6-phosphate isomerase Homo sapiens 88-91 23201122-3 2013 As pH(i) decreases, histones are globally deacetylated by histone deacetylases (HDACs), and the released acetate anions are coexported with protons out of the cell by monocarboxylate transporters (MCTs), preventing further reductions in pH(i). Acetates 105-112 glucose-6-phosphate isomerase Homo sapiens 237-242 23201122-5 2013 Inhibition of HDACs or MCTs decreases acetate export and lowers pH(i), particularly compromising pH(i) maintenance in acidic environments. mcts 23-27 glucose-6-phosphate isomerase Homo sapiens 64-69 23201122-5 2013 Inhibition of HDACs or MCTs decreases acetate export and lowers pH(i), particularly compromising pH(i) maintenance in acidic environments. mcts 23-27 glucose-6-phosphate isomerase Homo sapiens 97-102 22962303-8 2012 Bafilomycin A1, a specific V-ATPase inhibitor, markedly slowed the intracellular pH (pHi) recovery after acid load in human HCC cells and retarded the growth of human HCC in orthotopic xenograft model. bafilomycin A1 0-14 glucose-6-phosphate isomerase Homo sapiens 85-88 24429815-4 2013 Cell exposure to solutions containing NH3/NH4(+) elicits changes in intracellular pH (pHi), which can in turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss of solutes and water. Ammonia 38-41 glucose-6-phosphate isomerase Homo sapiens 82-84 24429815-4 2013 Cell exposure to solutions containing NH3/NH4(+) elicits changes in intracellular pH (pHi), which can in turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss of solutes and water. Ammonia 38-41 glucose-6-phosphate isomerase Homo sapiens 86-89 24429815-4 2013 Cell exposure to solutions containing NH3/NH4(+) elicits changes in intracellular pH (pHi), which can in turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss of solutes and water. Ammonia 42-45 glucose-6-phosphate isomerase Homo sapiens 82-84 24429815-4 2013 Cell exposure to solutions containing NH3/NH4(+) elicits changes in intracellular pH (pHi), which can in turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss of solutes and water. Ammonia 42-45 glucose-6-phosphate isomerase Homo sapiens 86-89 24429815-4 2013 Cell exposure to solutions containing NH3/NH4(+) elicits changes in intracellular pH (pHi), which can in turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss of solutes and water. Water 122-127 glucose-6-phosphate isomerase Homo sapiens 82-84 23536911-5 2013 Reduction of Gj was prevented completely by restricting the change of both intracellular calcium ([Ca(2+)]i) and pH (pHi) with potassium phosphate buffer. potassium phosphate 127-146 glucose-6-phosphate isomerase Homo sapiens 113-115 23536911-5 2013 Reduction of Gj was prevented completely by restricting the change of both intracellular calcium ([Ca(2+)]i) and pH (pHi) with potassium phosphate buffer. potassium phosphate 127-146 glucose-6-phosphate isomerase Homo sapiens 117-120 23536911-6 2013 Clamping of either [Ca(2+)]i or pHi, through BAPTA (2 mM) or HEPES (80 mM) respectively, offered partial resistance to ischemic uncoupling. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 45-50 glucose-6-phosphate isomerase Homo sapiens 32-35 23536911-6 2013 Clamping of either [Ca(2+)]i or pHi, through BAPTA (2 mM) or HEPES (80 mM) respectively, offered partial resistance to ischemic uncoupling. HEPES 61-66 glucose-6-phosphate isomerase Homo sapiens 32-35 23190385-3 2013 We tested the hypothesis that CNIs suppress cyclooxygenase (COX)-2-derived prostacyclin (PGI) and increase thromboxane synthesis in humans. Epoprostenol 75-87 glucose-6-phosphate isomerase Homo sapiens 89-92 24429815-4 2013 Cell exposure to solutions containing NH3/NH4(+) elicits changes in intracellular pH (pHi), which can in turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss of solutes and water. Water 122-127 glucose-6-phosphate isomerase Homo sapiens 86-89 24429815-4 2013 Cell exposure to solutions containing NH3/NH4(+) elicits changes in intracellular pH (pHi), which can in turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss of solutes and water. Water 221-226 glucose-6-phosphate isomerase Homo sapiens 82-84 24429815-4 2013 Cell exposure to solutions containing NH3/NH4(+) elicits changes in intracellular pH (pHi), which can in turn affect cell water volume (CWV) by activating transport mechanisms that produce net gain or loss of solutes and water. Water 221-226 glucose-6-phosphate isomerase Homo sapiens 86-89 24429815-9 2013 NH4Cl solutions in which either extracellular [NH3] or [NH4(+)] was kept constant while the other was changed by varying the pH of the solution, demonstrated that [NH3]o rather than [NH4(+)]o is the main determinant of the NH4Cl-induced swelling. Ammonium Chloride 0-5 glucose-6-phosphate isomerase Homo sapiens 125-127 24429815-9 2013 NH4Cl solutions in which either extracellular [NH3] or [NH4(+)] was kept constant while the other was changed by varying the pH of the solution, demonstrated that [NH3]o rather than [NH4(+)]o is the main determinant of the NH4Cl-induced swelling. Ammonia 0-3 glucose-6-phosphate isomerase Homo sapiens 125-127 22808997-2 2012 The title compound exhibits strong (epsilon 5 x 10(4) M(-1)cm(-1)) low-energy PDI acetylide-based pi-pi* absorption bands in the visible range extending to 600 nm, producing highly quenched singlet fluorescence (Phi = 0.014 +- 0.001, tau = 109 ps) with respect to a nonmetalated PDI model chromophore. acetylide 84-93 glucose-6-phosphate isomerase Homo sapiens 214-217 22728776-3 2012 WHO"s Public Health, Innovation and Intellectual Property Department (PHI) was established to address healthcare resource need for developing countries, assess impact of innovation and IP on access to medicines, explore innovative funding mechanisms for R&D and provide evidence-based policy-making recommendations in response to the changing global health landscape. Adenosine Monophosphate 256-259 glucose-6-phosphate isomerase Homo sapiens 70-73 23667903-2 2012 Human TIMP-1 fused to a glycosylphosphatidylinositol(GPI) anchor (TIMP-1 - GPI) shifts the activity of TIMP-1 from the extracellular matrix to the cell surface. Glycosylphosphatidylinositols 24-52 glucose-6-phosphate isomerase Homo sapiens 53-56 23667903-2 2012 Human TIMP-1 fused to a glycosylphosphatidylinositol(GPI) anchor (TIMP-1 - GPI) shifts the activity of TIMP-1 from the extracellular matrix to the cell surface. Glycosylphosphatidylinositols 24-52 glucose-6-phosphate isomerase Homo sapiens 75-78 23297504-5 2012 Compared with the placebo controls, the patients treated with dapoxetine on demand showed significant improvement in IELT (WMD = 1.39, 95% CI [1.23, 1.55], P < 0.000 01), PGI (OR = 2.59, 95% CI [2.21, 3.04], P < 0. dapoxetine 62-72 glucose-6-phosphate isomerase Homo sapiens 174-177 23297504-7 2012 There were significant differences between the 60 mg and 30 mg dapoxetine groups in IELT (WMD = 0.46, 95% CI [0.19, 0.74], P = 0.001 0) and PGI (OR = 1.32, 95% CI [1.06, 1.64], P = 0.01), but not in CCCB (OR = 1.39, 95% CI [0.90, 2.15], P = 0.13). dapoxetine 63-73 glucose-6-phosphate isomerase Homo sapiens 140-143 23297504-8 2012 CONCLUSION: Dapoxetine on demand can prolong IELT and improve PGI and CCCB, either at the dose of 60 mg or 30 mg, and has an even better efficacy in prolonging IELT and improving PGI at 60 mg. dapoxetine 12-22 glucose-6-phosphate isomerase Homo sapiens 62-65 23297504-8 2012 CONCLUSION: Dapoxetine on demand can prolong IELT and improve PGI and CCCB, either at the dose of 60 mg or 30 mg, and has an even better efficacy in prolonging IELT and improving PGI at 60 mg. dapoxetine 12-22 glucose-6-phosphate isomerase Homo sapiens 179-182 22808997-2 2012 The title compound exhibits strong (epsilon 5 x 10(4) M(-1)cm(-1)) low-energy PDI acetylide-based pi-pi* absorption bands in the visible range extending to 600 nm, producing highly quenched singlet fluorescence (Phi = 0.014 +- 0.001, tau = 109 ps) with respect to a nonmetalated PDI model chromophore. singlet 192-199 glucose-6-phosphate isomerase Homo sapiens 214-217 22808997-6 2012 Formation of the PDI triplet excited state in the title chromophore was also supported by sensitization of the singlet oxygen photoluminescence centered at ~1275 nm in air-saturated acetonitrile solution, Phi((1)O(2)) = 0.52. Singlet Oxygen 112-126 glucose-6-phosphate isomerase Homo sapiens 206-209 22681542-8 2012 Effective quantum efficiency (Phi) for the formation of e(aq)(-) in the process was determined to be 0.116 +- 0.002 mol/einstein. e(aq 56-60 glucose-6-phosphate isomerase Homo sapiens 30-33 24750936-1 2012 In current study, a water-soluble polysaccharide (GP-I), with a molecular mass of 33 kDa, was purified from Gynostemma pentaphyllum. Water 20-25 glucose-6-phosphate isomerase Homo sapiens 50-54 24750936-1 2012 In current study, a water-soluble polysaccharide (GP-I), with a molecular mass of 33 kDa, was purified from Gynostemma pentaphyllum. Polysaccharides 34-48 glucose-6-phosphate isomerase Homo sapiens 50-54 24750936-3 2012 The GP-I (25, 50, 100, 200 and 400 mug/ml) was found to have significant anti-proliferative effects on HaCat cells in a dose-dependent manner, as measured by MTT assay. monooxyethylene trimethylolpropane tristearate 158-161 glucose-6-phosphate isomerase Homo sapiens 4-8 24750936-4 2012 On the contrary, Trypan blue exclusion experiment indicated that GP-I had no cytotoxicity to HaCat cells. Trypan Blue 17-28 glucose-6-phosphate isomerase Homo sapiens 65-69 22474310-6 2012 The relationship between the mean DABF during XC and gastric pHi after XC release and postoperative gastrointestinal recovery was analysed with Spearman"s correlation coefficient. dabf 34-38 glucose-6-phosphate isomerase Homo sapiens 61-64 22474310-8 2012 There were two distinct relationships between DABF during XC and gastric pHi after XC release. dabf 46-50 glucose-6-phosphate isomerase Homo sapiens 73-76 22474310-9 2012 Gastric pHi steeply and linearly declined when indexed DABF was below 0.82 litre min(-1) m(-2). dabf 55-59 glucose-6-phosphate isomerase Homo sapiens 8-11 22366186-1 2012 Regulation of intracellular pH (pHi) and protection against cytosolic acidification is primarily a function of the ubiquitous plasma membrane Na+/H+exchanger-1 (NHE1), which uses a highly conserved process to transfer cytosolic hydrogen ions (H+) across plasma membranes in exchange for extracellular sodium ions (Na+). Hydrogen 228-236 glucose-6-phosphate isomerase Homo sapiens 32-35 23003635-2 2012 To investigate this question, we measure, by exploiting the well-established weak localization physics, the phase coherence length L(Phi) in dilute fluorinated graphene. Graphite 160-168 glucose-6-phosphate isomerase Homo sapiens 133-136 22278334-1 2012 Nonsteroidal anti-inflammatory drugs (NSAIDs) elevate cardiovascular risk by disrupting cyclooxygenase-2 (COX-2)-dependent biosynthesis of prostacyclin (PGI(2)). Epoprostenol 139-151 glucose-6-phosphate isomerase Homo sapiens 153-156 22278334-5 2012 Both CG100649 and celecoxib had the effect of depressing urinary excretion of 2,3-dinor-6-keto-PGF(1alpha) (PGI-M); the effect of CG100649 was dose-dependent and more sustained (up to 240 h after the dose) than that of celecoxib. CG100649 5-13 glucose-6-phosphate isomerase Homo sapiens 108-111 22278334-5 2012 Both CG100649 and celecoxib had the effect of depressing urinary excretion of 2,3-dinor-6-keto-PGF(1alpha) (PGI-M); the effect of CG100649 was dose-dependent and more sustained (up to 240 h after the dose) than that of celecoxib. Celecoxib 18-27 glucose-6-phosphate isomerase Homo sapiens 108-111 22278334-5 2012 Both CG100649 and celecoxib had the effect of depressing urinary excretion of 2,3-dinor-6-keto-PGF(1alpha) (PGI-M); the effect of CG100649 was dose-dependent and more sustained (up to 240 h after the dose) than that of celecoxib. 2,3-dinor-6-keto-pgf 78-98 glucose-6-phosphate isomerase Homo sapiens 108-111 22311857-10 2012 Further complicating attempts to define an optimal pHe is that media components can impact intracellular pH (pHi). Phenylalanine 51-54 glucose-6-phosphate isomerase Homo sapiens 109-112 22366186-1 2012 Regulation of intracellular pH (pHi) and protection against cytosolic acidification is primarily a function of the ubiquitous plasma membrane Na+/H+exchanger-1 (NHE1), which uses a highly conserved process to transfer cytosolic hydrogen ions (H+) across plasma membranes in exchange for extracellular sodium ions (Na+). Sodium 301-307 glucose-6-phosphate isomerase Homo sapiens 32-35 22311857-11 2012 As a result, media with different concentrations of substances, such as lactate or amino acids, may have different pHi, despite being in the same pHe. Lactic Acid 72-79 glucose-6-phosphate isomerase Homo sapiens 115-118 22469012-7 2012 The ethanol (1b, Phi = 2%) and toluene (1c, Phi = 10%) solvates of [Au(2)Cu(2)(C(2)OHC(5)H(8))(4)](n) adopt octanuclear isomeric structures (n = 2), while 1d (Phi = 25%) is a solvent-free chain polymer built from two types of Au(4)Cu(4) units. Ethanol 4-11 glucose-6-phosphate isomerase Homo sapiens 17-20 22339672-2 2012 As glutamate induces a Ca2+-dependent cytosolic acidification, the present work tested the relationships among intracellular Ca2+ concentration ([Ca2+](i)), intracellular pH (pH(i) ), and [Zn2+](i). Glutamic Acid 3-12 glucose-6-phosphate isomerase Homo sapiens 175-180 22339672-4 2012 Glu/Gly applications decreased pH(i) to 6.1 and induced intracellular Zn2+ release in a Ca2+-dependent manner, as expected. Glutamic Acid 0-3 glucose-6-phosphate isomerase Homo sapiens 31-36 22339672-4 2012 Glu/Gly applications decreased pH(i) to 6.1 and induced intracellular Zn2+ release in a Ca2+-dependent manner, as expected. Glycine 4-7 glucose-6-phosphate isomerase Homo sapiens 31-36 22339672-5 2012 The pH(i) drop reduced the affinity of FluoZin-3 and Fura-2-FF for Zn2+. FluoZin-3 39-48 glucose-6-phosphate isomerase Homo sapiens 4-9 22339672-5 2012 The pH(i) drop reduced the affinity of FluoZin-3 and Fura-2-FF for Zn2+. fura-2-ff 53-62 glucose-6-phosphate isomerase Homo sapiens 4-9 22339672-5 2012 The pH(i) drop reduced the affinity of FluoZin-3 and Fura-2-FF for Zn2+. Zinc 67-71 glucose-6-phosphate isomerase Homo sapiens 4-9 22339672-9 2012 Inhibiting the mechanisms responsible for the Ca2+-dependent pH(i) drop (plasmalemmal Ca2+ pump and mitochondria) counteracted the Glu/Gly-induced intracellular Zn2+ release. Glutamic Acid 131-134 glucose-6-phosphate isomerase Homo sapiens 61-66 22339672-9 2012 Inhibiting the mechanisms responsible for the Ca2+-dependent pH(i) drop (plasmalemmal Ca2+ pump and mitochondria) counteracted the Glu/Gly-induced intracellular Zn2+ release. Glycine 135-138 glucose-6-phosphate isomerase Homo sapiens 61-66 22339672-9 2012 Inhibiting the mechanisms responsible for the Ca2+-dependent pH(i) drop (plasmalemmal Ca2+ pump and mitochondria) counteracted the Glu/Gly-induced intracellular Zn2+ release. Zinc 161-165 glucose-6-phosphate isomerase Homo sapiens 61-66 22339672-11 2012 A pH(i) drop to 6.0 (without any Ca2+ influx or glutamate receptor activation) led to intracellular Zn2+ release; the released Zn2+ (free Zn2+ plus Zn2+) bound to Fura-2FF and FluoZin-3) reached 1 muM. Zinc 100-104 glucose-6-phosphate isomerase Homo sapiens 2-7 22339672-11 2012 A pH(i) drop to 6.0 (without any Ca2+ influx or glutamate receptor activation) led to intracellular Zn2+ release; the released Zn2+ (free Zn2+ plus Zn2+) bound to Fura-2FF and FluoZin-3) reached 1 muM. Zinc 127-131 glucose-6-phosphate isomerase Homo sapiens 2-7 22339672-11 2012 A pH(i) drop to 6.0 (without any Ca2+ influx or glutamate receptor activation) led to intracellular Zn2+ release; the released Zn2+ (free Zn2+ plus Zn2+) bound to Fura-2FF and FluoZin-3) reached 1 muM. Zinc 127-131 glucose-6-phosphate isomerase Homo sapiens 2-7 22339672-11 2012 A pH(i) drop to 6.0 (without any Ca2+ influx or glutamate receptor activation) led to intracellular Zn2+ release; the released Zn2+ (free Zn2+ plus Zn2+) bound to Fura-2FF and FluoZin-3) reached 1 muM. Zinc 127-131 glucose-6-phosphate isomerase Homo sapiens 2-7 22339672-11 2012 A pH(i) drop to 6.0 (without any Ca2+ influx or glutamate receptor activation) led to intracellular Zn2+ release; the released Zn2+ (free Zn2+ plus Zn2+) bound to Fura-2FF and FluoZin-3) reached 1 muM. Fura F 163-171 glucose-6-phosphate isomerase Homo sapiens 2-7 22339672-11 2012 A pH(i) drop to 6.0 (without any Ca2+ influx or glutamate receptor activation) led to intracellular Zn2+ release; the released Zn2+ (free Zn2+ plus Zn2+) bound to Fura-2FF and FluoZin-3) reached 1 muM. FluoZin-3 176-185 glucose-6-phosphate isomerase Homo sapiens 2-7 22469012-7 2012 The ethanol (1b, Phi = 2%) and toluene (1c, Phi = 10%) solvates of [Au(2)Cu(2)(C(2)OHC(5)H(8))(4)](n) adopt octanuclear isomeric structures (n = 2), while 1d (Phi = 25%) is a solvent-free chain polymer built from two types of Au(4)Cu(4) units. Toluene 31-38 glucose-6-phosphate isomerase Homo sapiens 44-47 22469012-7 2012 The ethanol (1b, Phi = 2%) and toluene (1c, Phi = 10%) solvates of [Au(2)Cu(2)(C(2)OHC(5)H(8))(4)](n) adopt octanuclear isomeric structures (n = 2), while 1d (Phi = 25%) is a solvent-free chain polymer built from two types of Au(4)Cu(4) units. Toluene 31-38 glucose-6-phosphate isomerase Homo sapiens 44-47 22469012-7 2012 The ethanol (1b, Phi = 2%) and toluene (1c, Phi = 10%) solvates of [Au(2)Cu(2)(C(2)OHC(5)H(8))(4)](n) adopt octanuclear isomeric structures (n = 2), while 1d (Phi = 25%) is a solvent-free chain polymer built from two types of Au(4)Cu(4) units. Gold 68-70 glucose-6-phosphate isomerase Homo sapiens 44-47 22469012-7 2012 The ethanol (1b, Phi = 2%) and toluene (1c, Phi = 10%) solvates of [Au(2)Cu(2)(C(2)OHC(5)H(8))(4)](n) adopt octanuclear isomeric structures (n = 2), while 1d (Phi = 25%) is a solvent-free chain polymer built from two types of Au(4)Cu(4) units. Gold 68-70 glucose-6-phosphate isomerase Homo sapiens 44-47 21191669-1 2012 Cancer cells maintain low intracellular pH [pHi]; therefore, it is likely that resveratrol [RSVL] inhibits cell growth through interference with regulation of pHi. Resveratrol 79-90 glucose-6-phosphate isomerase Homo sapiens 44-47 22227371-6 2012 Based on the activities of phosphoglucose isomerase and glucose-6 phosphate dehydrogenase we demonstrate that, relative to the capacity for entry into glycolysis, vanadium-accumulating species have enhanced capacity to metabolize glucose-6 phosphate via the PPP compared to non-accumulators. Vanadium 163-171 glucose-6-phosphate isomerase Homo sapiens 27-51 22131382-1 2012 According to traditional models of the basal ganglia-thalamocortical network of connections, dopamine exerts D2-like receptor (D2LR)-mediated effects through actions on striatal neurons that give rise to the "indirect" pathway, secondarily affecting the activity in the internal and external pallidal segments (GPi and GPe, respectively) and the substantia nigra pars reticulata (SNr). Dopamine 93-101 glucose-6-phosphate isomerase Homo sapiens 311-314 22131382-5 2012 In contrast, local application of quinpirole reduced firing in GPi and SNr, possibly through D2LR-mediated effects on glutamatergic inputs. Quinpirole 34-44 glucose-6-phosphate isomerase Homo sapiens 63-66 22131382-6 2012 Injections of the D2LR antagonist sulpiride resulted in effects opposite to those of quinpirole in GPe and GPi. Sulpiride 34-43 glucose-6-phosphate isomerase Homo sapiens 107-110 21191669-1 2012 Cancer cells maintain low intracellular pH [pHi]; therefore, it is likely that resveratrol [RSVL] inhibits cell growth through interference with regulation of pHi. Resveratrol 79-90 glucose-6-phosphate isomerase Homo sapiens 159-162 21191669-1 2012 Cancer cells maintain low intracellular pH [pHi]; therefore, it is likely that resveratrol [RSVL] inhibits cell growth through interference with regulation of pHi. Resveratrol 92-96 glucose-6-phosphate isomerase Homo sapiens 159-162 22017173-6 2011 R(0) decreased strongly as the contact work function (Phi) increased for both monothiol and dithiol junctions, whereas beta was independent of Phi within error. monothiol 78-87 glucose-6-phosphate isomerase Homo sapiens 54-57 22178061-8 2012 In macrophages, increased expression levels and co-localization of ferroportin and GPI-ceruloplasmin in cell surface lipid rafts were observed after iron treatment. Iron 149-153 glucose-6-phosphate isomerase Homo sapiens 83-86 22178061-12 2012 In macrophages, GPI-ceruloplasmin and ferroportin likely interact in lipid rafts to export iron from cells. Iron 91-95 glucose-6-phosphate isomerase Homo sapiens 16-19 22142544-2 2012 Introduction of a methoxy group into the perhydrophenanthrene skeleton was successfully achieved via a PhI(OAc)(2)-mediated phenolic oxidation of a benzocyclobutene nucleus and subsequent tandem intramolecular electrocyclic reactions based on o-quinodimethane chemistry. Tetradecahydrophenanthrene 41-61 glucose-6-phosphate isomerase Homo sapiens 103-106 22142544-2 2012 Introduction of a methoxy group into the perhydrophenanthrene skeleton was successfully achieved via a PhI(OAc)(2)-mediated phenolic oxidation of a benzocyclobutene nucleus and subsequent tandem intramolecular electrocyclic reactions based on o-quinodimethane chemistry. benzocyclobutene 148-164 glucose-6-phosphate isomerase Homo sapiens 103-106 22142544-2 2012 Introduction of a methoxy group into the perhydrophenanthrene skeleton was successfully achieved via a PhI(OAc)(2)-mediated phenolic oxidation of a benzocyclobutene nucleus and subsequent tandem intramolecular electrocyclic reactions based on o-quinodimethane chemistry. o-Xylylene 243-259 glucose-6-phosphate isomerase Homo sapiens 103-106 22563426-3 2012 Doxorubicin uptake into colon HCT116 cells was measured using the drug"s intrinsic fluorescence under conditions that alter pH(i) and pH(e) or pH(i) alone. Doxorubicin 0-11 glucose-6-phosphate isomerase Homo sapiens 143-148 22563426-4 2012 Acutely, doxorubicin influx across the cell-membrane correlates with the trans-membrane pH-gradient (facilitated at alkaline pH(e) and acidic pH(i)). Doxorubicin 9-20 glucose-6-phosphate isomerase Homo sapiens 142-147 22563426-8 2012 Measurements of cell proliferation demonstrate that doxorubicin efficacy is enhanced at alkaline pH(i) and that pH-partition theory is inadequate to account for this. Doxorubicin 52-63 glucose-6-phosphate isomerase Homo sapiens 97-102 22563426-10 2012 In summary, doxorubicin uptake into cells is favoured by high pH(e) and high pH(i). Doxorubicin 12-23 glucose-6-phosphate isomerase Homo sapiens 77-82 22360560-7 2012 A particularly important feature of tumour pHi regulation is acid-extrusion, which involves H+-extrusion and HCO3--uptake by membrane-bound transporter-proteins. Bicarbonates 109-113 glucose-6-phosphate isomerase Homo sapiens 43-46 23430879-1 2012 Primary hyperoxaluria type I (PH I) is a rare genetic disorder that leads to end stage renal disease (ESRD) at an early age due to excessive deposition of calcium oxalate in the kidney. Calcium Oxalate 155-170 glucose-6-phosphate isomerase Homo sapiens 0-28 23430879-1 2012 Primary hyperoxaluria type I (PH I) is a rare genetic disorder that leads to end stage renal disease (ESRD) at an early age due to excessive deposition of calcium oxalate in the kidney. Calcium Oxalate 155-170 glucose-6-phosphate isomerase Homo sapiens 30-34 23430879-9 2012 Sequential liver transplantation followed by kidney transplantation is to be considered for PH I patients who have ESRD and very high oxalate load. Oxalates 134-141 glucose-6-phosphate isomerase Homo sapiens 92-96 22563426-3 2012 Doxorubicin uptake into colon HCT116 cells was measured using the drug"s intrinsic fluorescence under conditions that alter pH(i) and pH(e) or pH(i) alone. Doxorubicin 0-11 glucose-6-phosphate isomerase Homo sapiens 124-129 22558080-5 2012 METHODS AND FINDING: By modifying human TIMP-1 through the addition of a glycosylphosphatidylinositol (GPI) anchor, a recombinant protein was generated that efficiently focuses TIMP-1 on the cell surface. Glycosylphosphatidylinositols 73-101 glucose-6-phosphate isomerase Homo sapiens 103-106 22384096-3 2012 In this study, we used bases and acetic acid to manipulate the pHi. Acetic Acid 33-44 glucose-6-phosphate isomerase Homo sapiens 63-66 21835227-10 2011 These changes may be in part due to changes in the GABA release in the GPe and GPi, but also involve intrinsic cellular changes in pallidal neurons. gamma-Aminobutyric Acid 51-55 glucose-6-phosphate isomerase Homo sapiens 79-82 22017173-6 2011 R(0) decreased strongly as the contact work function (Phi) increased for both monothiol and dithiol junctions, whereas beta was independent of Phi within error. dithiol 92-99 glucose-6-phosphate isomerase Homo sapiens 54-57 21920515-0 2011 Synthesis of octyl S-glycosides of tri- to pentasaccharide fragments related to the GPI anchor of Trypanosoma brucei. octyl s-glycosides 13-31 glucose-6-phosphate isomerase Homo sapiens 84-87 21595652-1 2011 BACKGROUND AND PURPOSE: Na(+) /HCO(3) (-) co-transport (NBC) regulates intracellular pH (pH(i) ) in the heart. 7 alpha-hydroxy-4-cholesten-3-one 31-34 glucose-6-phosphate isomerase Homo sapiens 89-94 21595652-6 2011 KEY RESULTS: The potassium pulse produced a pH(i) increase of 0.18 +- 0.006 (n= 5), which was reduced by the a-L3 antibody (0.016 +- 0.019). Potassium 17-26 glucose-6-phosphate isomerase Homo sapiens 44-49 22146403-4 2011 The aims of the present study were to investigate the physiological mechanisms of pHi recovery and to further explore the effects of alcohol on the pHi in human umbilical cord blood CD34 stem cell-like cells (HUCB-CD34STs). Alcohols 133-140 glucose-6-phosphate isomerase Homo sapiens 148-151 21907557-3 2011 The low DC potential used decreased the interfering species effect and the biosensor showed a linear impedance response toward glucose detection at concentrations from 0mM to 20mM,with 0.9853 and 0.9945 correlation coefficient for impedance module ( Z ) and phase (Phi), respectively. Glucose 127-134 glucose-6-phosphate isomerase Homo sapiens 265-268 21920515-0 2011 Synthesis of octyl S-glycosides of tri- to pentasaccharide fragments related to the GPI anchor of Trypanosoma brucei. tri- to 35-42 glucose-6-phosphate isomerase Homo sapiens 84-87 21920515-0 2011 Synthesis of octyl S-glycosides of tri- to pentasaccharide fragments related to the GPI anchor of Trypanosoma brucei. pentasaccharide 43-58 glucose-6-phosphate isomerase Homo sapiens 84-87 21920515-7 2011 Saccharides 19, 27 and 37 are suitable substrates for studying the enzymatic glycosylation pattern of the GPI anchor of T. brucei. Carbohydrates 0-11 glucose-6-phosphate isomerase Homo sapiens 106-109 21903582-3 2011 Cells exposed to elevated CO(2) died in galactose medium as well as when glucose-6-phosphate isomerase was knocked down, suggesting mitochondrial dysfunction. co(2) 26-31 glucose-6-phosphate isomerase Homo sapiens 73-102 21932835-3 2011 Fluorescence, resonant light scattering (RLS) and (1)H NMR experiments, as well as X-ray crystallographic have demonstrated that the configurational arrangement of the synthesized chlorins prevent pi-stacking interactions between macrocycles, thus indicating that it is a nonaggregating photosensitizer with high singlet oxygen (Phi(Delta)) and fluorescence (Phi(f)) quantum yields. chlorin 180-188 glucose-6-phosphate isomerase Homo sapiens 329-339 21413028-3 2011 We addressed the mechanism by which cells manage this acid load by measuring intracellular pH (pHi) in non-transformed osteoblasts in response to weak acid or bicarbonate loading. weak acid 146-155 glucose-6-phosphate isomerase Homo sapiens 95-98 21645513-2 2011 Our study found that diphyllin can inhibit the expression of V-ATPases in a dose-dependent manner, decrease the internal pH (pHi) and reverse the transmembrane pH gradient in SGC7901 cells. diphyllin 21-30 glucose-6-phosphate isomerase Homo sapiens 125-128 21773906-5 2011 Additional immunohistochemical staining of glucose transporter type 1 (GLUT1) and the autocrine motility factor (AMF) was conducted after the tumors were resected, to identify the mechanism that increased FDG uptake on PET. Fluorodeoxyglucose F18 205-208 glucose-6-phosphate isomerase Homo sapiens 113-116 21576809-3 2011 By identifying the saturation value with the work function of graphite we determine the work function of single and bilayer graphene to be Phi(SLG) = 4.409 +- 0.039 eV and Phi(BLG) = 4.516 +- 0.035 eV, respectively. Graphite 62-70 glucose-6-phosphate isomerase Homo sapiens 139-142 21576809-3 2011 By identifying the saturation value with the work function of graphite we determine the work function of single and bilayer graphene to be Phi(SLG) = 4.409 +- 0.039 eV and Phi(BLG) = 4.516 +- 0.035 eV, respectively. Graphite 62-70 glucose-6-phosphate isomerase Homo sapiens 172-175 21576809-3 2011 By identifying the saturation value with the work function of graphite we determine the work function of single and bilayer graphene to be Phi(SLG) = 4.409 +- 0.039 eV and Phi(BLG) = 4.516 +- 0.035 eV, respectively. Graphite 124-132 glucose-6-phosphate isomerase Homo sapiens 139-142 21252914-0 2011 Autocrine motility factor/phosphoglucose isomerase regulates ER stress and cell death through control of ER calcium release. Calcium 108-115 glucose-6-phosphate isomerase Homo sapiens 26-50 21252914-3 2011 We demonstrate here that AMF/PGI also protects against thapsigargin (TG)- and tunicamycin (TUN)-induced ER stress and apoptosis. Thapsigargin 55-67 glucose-6-phosphate isomerase Homo sapiens 29-32 21252914-3 2011 We demonstrate here that AMF/PGI also protects against thapsigargin (TG)- and tunicamycin (TUN)-induced ER stress and apoptosis. Thapsigargin 69-71 glucose-6-phosphate isomerase Homo sapiens 29-32 21252914-3 2011 We demonstrate here that AMF/PGI also protects against thapsigargin (TG)- and tunicamycin (TUN)-induced ER stress and apoptosis. Tunicamycin 78-89 glucose-6-phosphate isomerase Homo sapiens 29-32 21252914-3 2011 We demonstrate here that AMF/PGI also protects against thapsigargin (TG)- and tunicamycin (TUN)-induced ER stress and apoptosis. Tunicamycin 91-94 glucose-6-phosphate isomerase Homo sapiens 29-32 21252914-6 2011 AMF/PGI reduction of the elevation of cytosolic calcium in response to either TG or inositol 1,4,5-trisphosphate receptor activation with ATP was gp78/AMFR-dependent, independent of mitochondrial depolarization and not associated with changes in ER calcium content. Calcium 48-55 glucose-6-phosphate isomerase Homo sapiens 4-7 21252914-6 2011 AMF/PGI reduction of the elevation of cytosolic calcium in response to either TG or inositol 1,4,5-trisphosphate receptor activation with ATP was gp78/AMFR-dependent, independent of mitochondrial depolarization and not associated with changes in ER calcium content. Thapsigargin 78-80 glucose-6-phosphate isomerase Homo sapiens 4-7 21252914-6 2011 AMF/PGI reduction of the elevation of cytosolic calcium in response to either TG or inositol 1,4,5-trisphosphate receptor activation with ATP was gp78/AMFR-dependent, independent of mitochondrial depolarization and not associated with changes in ER calcium content. Adenosine Triphosphate 138-141 glucose-6-phosphate isomerase Homo sapiens 4-7 21252914-6 2011 AMF/PGI reduction of the elevation of cytosolic calcium in response to either TG or inositol 1,4,5-trisphosphate receptor activation with ATP was gp78/AMFR-dependent, independent of mitochondrial depolarization and not associated with changes in ER calcium content. Calcium 249-256 glucose-6-phosphate isomerase Homo sapiens 4-7 21252914-7 2011 These results implicate regulation of ER calcium release in AMF/PGI protection against ER stress and apoptosis. Calcium 41-48 glucose-6-phosphate isomerase Homo sapiens 64-67 21252914-9 2011 Importantly, elevation of cytosolic calcium upon treatment with the calcium ionophore ionomycin, while not inducing an ER stress response, did prevent AMF/PGI protection against ER stress. Calcium 36-43 glucose-6-phosphate isomerase Homo sapiens 155-158 21252914-9 2011 Importantly, elevation of cytosolic calcium upon treatment with the calcium ionophore ionomycin, while not inducing an ER stress response, did prevent AMF/PGI protection against ER stress. Calcium 68-75 glucose-6-phosphate isomerase Homo sapiens 155-158 21252914-9 2011 Importantly, elevation of cytosolic calcium upon treatment with the calcium ionophore ionomycin, while not inducing an ER stress response, did prevent AMF/PGI protection against ER stress. Ionomycin 86-95 glucose-6-phosphate isomerase Homo sapiens 155-158 21252914-10 2011 By regulating ER calcium release, AMF/PGI interaction with gp78/AMFR therefore protects against ER stress identifying novel roles for these cancer-associated proteins in promoting tumor cell survival. Calcium 17-24 glucose-6-phosphate isomerase Homo sapiens 38-41 21419765-3 2011 To further examine this question, we investigated the anatomical localization of GABA-B receptors and the electrophysiologic effects of microinjections of GABA-B receptor ligands in GPe and GPi of MPTP-treated (parkinsonian) monkeys. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 197-201 glucose-6-phosphate isomerase Homo sapiens 190-193 21419765-5 2011 However, the magnitude of reduction in firing rate of GPe and GPi neurons produced by microinjections of the GABA-B receptor agonist baclofen was larger in MPTP-treated animals than in normal monkeys. Baclofen 133-141 glucose-6-phosphate isomerase Homo sapiens 62-65 21419765-5 2011 However, the magnitude of reduction in firing rate of GPe and GPi neurons produced by microinjections of the GABA-B receptor agonist baclofen was larger in MPTP-treated animals than in normal monkeys. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 156-160 glucose-6-phosphate isomerase Homo sapiens 62-65 21419765-7 2011 In addition, the injections of baclofen in GPe and GPi, or of CGP55845A in GPi lead to a significant increase in the proportion of spikes in rebound bursts in parkinsonian animals, but not in normal monkeys. Baclofen 31-39 glucose-6-phosphate isomerase Homo sapiens 51-54 21413028-3 2011 We addressed the mechanism by which cells manage this acid load by measuring intracellular pH (pHi) in non-transformed osteoblasts in response to weak acid or bicarbonate loading. Bicarbonates 159-170 glucose-6-phosphate isomerase Homo sapiens 95-98 21413028-4 2011 Basal pHi in mineralizing osteoblasts was ~ 7.3 and decreased by ~ 1.4 units upon replacing extracellular Na(+) with N-methyl-D-glucamine. Meglumine 117-137 glucose-6-phosphate isomerase Homo sapiens 6-9 21413028-8 2011 Sodium-dependent pHi recovery from weak acid loading was inhibited by amiloride with the Ki consistent with NHEs. Sodium 0-6 glucose-6-phosphate isomerase Homo sapiens 17-20 21413028-8 2011 Sodium-dependent pHi recovery from weak acid loading was inhibited by amiloride with the Ki consistent with NHEs. Amiloride 70-79 glucose-6-phosphate isomerase Homo sapiens 17-20 21519387-3 2011 Here, biochemical effects of STN-DBS have been assessed in putamen (PUT), internal pallidus (GPi), and inside the antero-ventral thalamus (VA), the key station receiving pallidothalamic fibers. stn-dbs 29-36 glucose-6-phosphate isomerase Homo sapiens 93-96 21599067-6 2011 We obtain initial quantum yield for ozone synthesis from solid oxygen, Phi(O(3)) = 0.24 +- 0.06, and quantum yields for destruction of O(3) and O(2) in their parent solids, Phi(-O(3)) = 1.0 +- 0.2 and Phi(-O(2)) = 0.36 +- 0.1. Ozone 36-41 glucose-6-phosphate isomerase Homo sapiens 71-74 21389093-7 2011 Collectively, these results suggest a role of miR-200s in PGI/AMF-induced EMT and thus approaches for upregulation of miR-200s could be a novel therapeutic strategy for the treatment of highly invasive breast cancer. mir-200s 46-54 glucose-6-phosphate isomerase Homo sapiens 58-61 21527629-1 2011 Leakiness (Phi), the proportion of carbon fixed by phosphoenolpyruvate carboxylation that leaks out of the bundle-sheath cells, determines C(4) photosynthetic efficiency. Carbon 35-41 glucose-6-phosphate isomerase Homo sapiens 11-14 21527629-1 2011 Leakiness (Phi), the proportion of carbon fixed by phosphoenolpyruvate carboxylation that leaks out of the bundle-sheath cells, determines C(4) photosynthetic efficiency. Phosphoenolpyruvate 51-70 glucose-6-phosphate isomerase Homo sapiens 11-14 21519387-5 2011 cGMP, an index of glutamatergic transmission, was measured in GPi and PUT by radioimmunoassay, whereas GABA from VA was measured by HPLC. Cyclic GMP 0-4 glucose-6-phosphate isomerase Homo sapiens 62-65 21519387-7 2011 Simultaneously, cGMP extracellular concentrations were enhanced in PUT (+200%) and GPi (+481%). Cyclic GMP 16-20 glucose-6-phosphate isomerase Homo sapiens 83-86 21078290-2 2011 The method uses a plot of M sin(Phi) versus M cos(Phi), where M is the modulation ratio and Phi is the phase angle taken from frequency domain fluorometry. carbonyl sulfide 46-49 glucose-6-phosphate isomerase Homo sapiens 50-53 21078290-2 2011 The method uses a plot of M sin(Phi) versus M cos(Phi), where M is the modulation ratio and Phi is the phase angle taken from frequency domain fluorometry. carbonyl sulfide 46-49 glucose-6-phosphate isomerase Homo sapiens 50-53 20845477-9 2011 Haplotype CGG of GPI and GCTATGG of HK2 were associated with better OS, respectively, with P values of .004 and .007. Osmium 68-70 glucose-6-phosphate isomerase Homo sapiens 17-20 21182530-2 2011 The quantum yields Phi(Delta) of singlet molecular oxygen formation of berberine, palmatine and sanguinarine are moderate in dichloromethane (0.2-0.6) and much smaller in acetonitrile or trifluoroethanol. Oxygen 51-57 glucose-6-phosphate isomerase Homo sapiens 19-22 21182530-2 2011 The quantum yields Phi(Delta) of singlet molecular oxygen formation of berberine, palmatine and sanguinarine are moderate in dichloromethane (0.2-0.6) and much smaller in acetonitrile or trifluoroethanol. Berberine 71-80 glucose-6-phosphate isomerase Homo sapiens 19-22 21182530-2 2011 The quantum yields Phi(Delta) of singlet molecular oxygen formation of berberine, palmatine and sanguinarine are moderate in dichloromethane (0.2-0.6) and much smaller in acetonitrile or trifluoroethanol. palmatine 82-91 glucose-6-phosphate isomerase Homo sapiens 19-22 21182530-2 2011 The quantum yields Phi(Delta) of singlet molecular oxygen formation of berberine, palmatine and sanguinarine are moderate in dichloromethane (0.2-0.6) and much smaller in acetonitrile or trifluoroethanol. sanguinarine 96-108 glucose-6-phosphate isomerase Homo sapiens 19-22 21182530-2 2011 The quantum yields Phi(Delta) of singlet molecular oxygen formation of berberine, palmatine and sanguinarine are moderate in dichloromethane (0.2-0.6) and much smaller in acetonitrile or trifluoroethanol. Methylene Chloride 125-140 glucose-6-phosphate isomerase Homo sapiens 19-22 21182530-2 2011 The quantum yields Phi(Delta) of singlet molecular oxygen formation of berberine, palmatine and sanguinarine are moderate in dichloromethane (0.2-0.6) and much smaller in acetonitrile or trifluoroethanol. acetonitrile 171-183 glucose-6-phosphate isomerase Homo sapiens 19-22 21182530-2 2011 The quantum yields Phi(Delta) of singlet molecular oxygen formation of berberine, palmatine and sanguinarine are moderate in dichloromethane (0.2-0.6) and much smaller in acetonitrile or trifluoroethanol. Trifluoroethanol 187-203 glucose-6-phosphate isomerase Homo sapiens 19-22 21190891-2 2011 Potential of hydrogen bond is the function which relates its energy to geometrical parameters of hydrogen bridge: its length R(O...O) and angles between direction O...O and OH group [phi (H-O...O)] and/or lone pair of proton accepting oxygen atom [chi(-O...O)]. Hydrogen 13-21 glucose-6-phosphate isomerase Homo sapiens 183-186 21152879-3 2011 Such a decrease of pHi was closely correlated with the dose of this phenoxazine and continued for 4 h. The activity of Na+/H+ exchanger isoform l (NHE1), which is involved in H+ extrusion from the cells, was dose-dependently suppressed by Phx-3 in these cells, and was greatly suppressed in the presence of 100 muM Phx-3. phenoxazine 68-79 glucose-6-phosphate isomerase Homo sapiens 19-22 21190891-2 2011 Potential of hydrogen bond is the function which relates its energy to geometrical parameters of hydrogen bridge: its length R(O...O) and angles between direction O...O and OH group [phi (H-O...O)] and/or lone pair of proton accepting oxygen atom [chi(-O...O)]. Hydrogen 97-105 glucose-6-phosphate isomerase Homo sapiens 183-186 21190891-2 2011 Potential of hydrogen bond is the function which relates its energy to geometrical parameters of hydrogen bridge: its length R(O...O) and angles between direction O...O and OH group [phi (H-O...O)] and/or lone pair of proton accepting oxygen atom [chi(-O...O)]. Oxygen 235-241 glucose-6-phosphate isomerase Homo sapiens 183-186 22027095-7 2011 This result could be explained, in part, by the fact that the insufficient supply of NADPH (nicotinamide adenine dinucleotide phosphate, reduced form) was caused by pHi decrease in neutrophils treated with Phx-1 or Phx, because NADPH is necessary for NADPH oxidase responsible for generating superoxide in the cells. NADP 85-90 glucose-6-phosphate isomerase Homo sapiens 165-168 20881233-6 2011 The [Ca2(+)](i)-suppressive effects of alkalinization were antagonized by the fast Ca2(+) buffer BAPTA, suggesting that pH(i) directly regulates Ca2(+) binding to internal anionic sites. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 97-102 glucose-6-phosphate isomerase Homo sapiens 120-125 20671312-5 2011 Recent developments suggest that these limitations may be overcome by encapsulation of therapeutic proteins into nanoparticles or their covalent modification with glycolipid (glycosylphosphatidylinositol, GPI) structures. Glycolipids 163-173 glucose-6-phosphate isomerase Homo sapiens 205-208 22027095-7 2011 This result could be explained, in part, by the fact that the insufficient supply of NADPH (nicotinamide adenine dinucleotide phosphate, reduced form) was caused by pHi decrease in neutrophils treated with Phx-1 or Phx, because NADPH is necessary for NADPH oxidase responsible for generating superoxide in the cells. NADP 92-135 glucose-6-phosphate isomerase Homo sapiens 165-168 20974851-7 2010 We also found that the genistein apparent dissociation constant for activation (K(a)) increased at alkaline pH(i), near cysteine pK (K(a) = 1.83, 1.81 and 4.99 mum at pH(i) 6, 7.35, and 8, respectively), suggesting the involvement of cysteines in the binding site. Genistein 23-32 glucose-6-phosphate isomerase Homo sapiens 108-113 20952463-5 2011 IgA anti-ss(2)-GPI (> 20 phospholipid units) was found in 20%. Phospholipids 28-40 glucose-6-phosphate isomerase Homo sapiens 15-18 21558757-5 2011 This rapid and drastic decrease of pHi in a variety of cancer cells caused by Phx-3 and Phx-1 possibly perturbed their intracellular homeostasis, and extensively affected the subsequent cell death, because these phenoxazines exerted dose-dependent proapoptotic and cytotoxic effects on these cells during 72 h incubation, confirming a causal relationship between DeltapHi and cytotoxic effects due to Phx-3 and Phx-1. phenoxazine 212-224 glucose-6-phosphate isomerase Homo sapiens 35-38 20974851-7 2010 We also found that the genistein apparent dissociation constant for activation (K(a)) increased at alkaline pH(i), near cysteine pK (K(a) = 1.83, 1.81 and 4.99 mum at pH(i) 6, 7.35, and 8, respectively), suggesting the involvement of cysteines in the binding site. Genistein 23-32 glucose-6-phosphate isomerase Homo sapiens 167-172 20974851-8 2010 Mutations of cysteine residues predicted to be within (Cys-491) or outside (Cys-1344) the potentiator-binding site showed that Cys-491 is responsible for the sensitivity of potentiator binding to alkaline pH(i). Cysteine 13-21 glucose-6-phosphate isomerase Homo sapiens 205-210 20860359-9 2010 The efficacy of the nickel procedure has been further applied toward the preparation of heparin disaccharides, GPI anchor pseudodisaccharides, and alpha-GluNAc/GalNAc. pseudodisaccharides 122-141 glucose-6-phosphate isomerase Homo sapiens 111-114 20978190-1 2010 Phosphoglucose isomerase (PGI) is a multifunctional enzyme that functions in glucose metabolism as a glycolytic enzyme catalyzing an interconversion between glucose and fructose inside the cell, while it acts as cytokine outside the cell, with properties that include autocrine motility factor (AMF)-regulating tumor cell motility. Glucose 7-14 glucose-6-phosphate isomerase Homo sapiens 26-29 20978190-1 2010 Phosphoglucose isomerase (PGI) is a multifunctional enzyme that functions in glucose metabolism as a glycolytic enzyme catalyzing an interconversion between glucose and fructose inside the cell, while it acts as cytokine outside the cell, with properties that include autocrine motility factor (AMF)-regulating tumor cell motility. Glucose 77-84 glucose-6-phosphate isomerase Homo sapiens 0-24 20978190-1 2010 Phosphoglucose isomerase (PGI) is a multifunctional enzyme that functions in glucose metabolism as a glycolytic enzyme catalyzing an interconversion between glucose and fructose inside the cell, while it acts as cytokine outside the cell, with properties that include autocrine motility factor (AMF)-regulating tumor cell motility. Glucose 77-84 glucose-6-phosphate isomerase Homo sapiens 26-29 21068538-1 2010 BACKGROUND: The addition of glycoprotein IIb/IIIa inhibitors (GPIs) to heparin in percutaneous coronary intervention (PCI) procedures has been demonstrated to reduce ischemic complications; however, GPI use is known to increase the risk of bleeding events, which are linked to increased mortality, longer hospital length of stay, greater medical resource utilization, and increased costs. Heparin 71-78 glucose-6-phosphate isomerase Homo sapiens 62-65 20435405-4 2010 cbeta(2)gpI strongly reduced HUVEC growth and proliferation as evidenced by the MTT and BrdU assay and delayed cell cycle progression arresting HUVEC in the S-and G2/M-phase. monooxyethylene trimethylolpropane tristearate 80-83 glucose-6-phosphate isomerase Homo sapiens 8-11 20435405-4 2010 cbeta(2)gpI strongly reduced HUVEC growth and proliferation as evidenced by the MTT and BrdU assay and delayed cell cycle progression arresting HUVEC in the S-and G2/M-phase. Bromodeoxyuridine 88-92 glucose-6-phosphate isomerase Homo sapiens 8-11 20860359-9 2010 The efficacy of the nickel procedure has been further applied toward the preparation of heparin disaccharides, GPI anchor pseudodisaccharides, and alpha-GluNAc/GalNAc. Nickel 20-26 glucose-6-phosphate isomerase Homo sapiens 111-114 21090576-3 2010 The dye substituted with four iodine atoms showed yields of Phi(T) = 0.78 and Phi((1)O(2)) = 0.70, which are the highest values so far obtained for the aza-BODIPY derivatives. Iodine 30-36 glucose-6-phosphate isomerase Homo sapiens 60-63 21090576-3 2010 The dye substituted with four iodine atoms showed yields of Phi(T) = 0.78 and Phi((1)O(2)) = 0.70, which are the highest values so far obtained for the aza-BODIPY derivatives. Iodine 30-36 glucose-6-phosphate isomerase Homo sapiens 78-81 21090576-3 2010 The dye substituted with four iodine atoms showed yields of Phi(T) = 0.78 and Phi((1)O(2)) = 0.70, which are the highest values so far obtained for the aza-BODIPY derivatives. azaBDPBA compound 152-162 glucose-6-phosphate isomerase Homo sapiens 60-63 21090576-3 2010 The dye substituted with four iodine atoms showed yields of Phi(T) = 0.78 and Phi((1)O(2)) = 0.70, which are the highest values so far obtained for the aza-BODIPY derivatives. azaBDPBA compound 152-162 glucose-6-phosphate isomerase Homo sapiens 78-81 20876775-2 2010 The major goal of this study was to determine the mechanism responsible for bile acid-induced alteration in intracellular pH (pH(i)) and its effect on DNA damage in cells derived from normal oesophagus (HET1A) or Barrett"s oesophagus (CP-A). Bile Acids and Salts 76-85 glucose-6-phosphate isomerase Homo sapiens 126-131 20876775-6 2010 RESULTS: A dose dependent decrease in pH(i) was observed in CP-A cells exposed to BA. Barium 82-84 glucose-6-phosphate isomerase Homo sapiens 38-43 20876775-8 2010 Exposure of oesophageal cells to acid in combination with BA synergistically decreased pH(i). Barium 58-60 glucose-6-phosphate isomerase Homo sapiens 87-92 20541516-5 2010 The preparation involves conversion of readily available, carrier-free d-[6,6"-(3)H]glucose to [6,6"-(3)H]Fru-2,6-P(2) using hexokinase, glucose-6-phosphate isomerase, and 6-phosphofructo-2-kinase. d-[6,6"-(3)h]glucose 71-91 glucose-6-phosphate isomerase Homo sapiens 137-166 21160910-1 2010 BACKGROUND: Sodium/hydrogen exchanger-1 (NHE-1) contributes to maintaining intracellular pH (pHi). Sodium 12-18 glucose-6-phosphate isomerase Homo sapiens 93-96 21160910-1 2010 BACKGROUND: Sodium/hydrogen exchanger-1 (NHE-1) contributes to maintaining intracellular pH (pHi). Hydrogen 19-27 glucose-6-phosphate isomerase Homo sapiens 93-96 21160910-6 2010 pHi was assessed pre- and post-incubation with glucose, insulin, leptin and adrenaline. Glucose 47-54 glucose-6-phosphate isomerase Homo sapiens 0-3 20713733-7 2010 Simultaneous dual color high-resolution images revealed that GPI anchored and certain transmembrane proteins were recruited to regions proximal (< 150 nm) to CTxB-GM1 nanodomains without physical intermixing. ctxb-gm1 161-169 glucose-6-phosphate isomerase Homo sapiens 61-64 20866447-3 2010 The compression occurs at lower crowding agent concentration, Phi when polymer molecular weight increases. Polymers 71-78 glucose-6-phosphate isomerase Homo sapiens 62-65 20866447-4 2010 The Flory exponent nu(Phi) decreases from nu(0) 0.48 in water down to 0.3 with macromolecular crowding. Water 56-61 glucose-6-phosphate isomerase Homo sapiens 22-25 20561412-7 2010 The pHi of HL-60 cells increased from 7.11 to 7.46 after treatment with etoposide for 24 hours. Etoposide 72-81 glucose-6-phosphate isomerase Homo sapiens 4-7 20351106-10 2010 We demonstrate that neutralization of a lysine residue (Lys(245)) located at the C-terminal end of transmembrane domain 4 by mutation to alanine abolishes gating by pH(i). Lysine 40-46 glucose-6-phosphate isomerase Homo sapiens 165-170 20351106-10 2010 We demonstrate that neutralization of a lysine residue (Lys(245)) located at the C-terminal end of transmembrane domain 4 by mutation to alanine abolishes gating by pH(i). Lysine 56-59 glucose-6-phosphate isomerase Homo sapiens 165-170 20351106-10 2010 We demonstrate that neutralization of a lysine residue (Lys(245)) located at the C-terminal end of transmembrane domain 4 by mutation to alanine abolishes gating by pH(i). Alanine 137-144 glucose-6-phosphate isomerase Homo sapiens 165-170 20351106-11 2010 We postulate that this lysine acts as an intracellular pH sensor as its mutation to histidine acid-shifts the pH(i)-dependence curve of TASK-2 as expected from its lower pK(a). Lysine 23-29 glucose-6-phosphate isomerase Homo sapiens 110-115 20351106-11 2010 We postulate that this lysine acts as an intracellular pH sensor as its mutation to histidine acid-shifts the pH(i)-dependence curve of TASK-2 as expected from its lower pK(a). histidine acid 84-98 glucose-6-phosphate isomerase Homo sapiens 110-115 20445242-6 2010 The crystal structure of Mtb PGI was observed to be rather more similar to human PGI than other nonbacterial PGIs, with only a few differences being detected in the loops, arm and hook regions of the human and Mtb PGIs, suggesting that the M. tuberculosis enzyme uses the same enzyme mechanism. Prostaglandins I 109-113 glucose-6-phosphate isomerase Homo sapiens 29-32 20407696-1 2010 Self-assembled monolayers (SAMs) of functionalized thiols are widely used in organic (opto)electronic devices to tune the work function, Phi, of noble-metal electrodes and, thereby, to optimize the barriers for charge-carrier injection. Sulfhydryl Compounds 51-57 glucose-6-phosphate isomerase Homo sapiens 137-140 20407696-2 2010 The achievable Phi values not only depend on the intrinsic molecular dipole moment of the thiols but, importantly, also on the bond dipole at the Au-S interface. Sulfhydryl Compounds 90-96 glucose-6-phosphate isomerase Homo sapiens 15-18 20407696-2 2010 The achievable Phi values not only depend on the intrinsic molecular dipole moment of the thiols but, importantly, also on the bond dipole at the Au-S interface. Gold 146-148 glucose-6-phosphate isomerase Homo sapiens 15-18 20407696-2 2010 The achievable Phi values not only depend on the intrinsic molecular dipole moment of the thiols but, importantly, also on the bond dipole at the Au-S interface. Sulfur 149-150 glucose-6-phosphate isomerase Homo sapiens 15-18 20357159-0 2010 Sedation with GPI 15715, a water-soluble prodrug of propofol, using target-controlled infusion in volunteers: retraction. Water 27-32 glucose-6-phosphate isomerase Homo sapiens 14-17 20357159-0 2010 Sedation with GPI 15715, a water-soluble prodrug of propofol, using target-controlled infusion in volunteers: retraction. Propofol 52-60 glucose-6-phosphate isomerase Homo sapiens 14-17 20126985-8 2010 These results suggest that a decrease of pHi, caused by depolarization of the mitochondria, may trigger the dysfunction of mitochondria causing ROS production; therefore, both the translocation of NF-kappaB from the cytoplasm to the nucleus and apoptosis induction were promoted in A549 cells. Reactive Oxygen Species 144-147 glucose-6-phosphate isomerase Homo sapiens 41-44 20208175-1 2010 Phosphoglucose isomerase (PGI) is a key enzyme in glycolysis and glycogenesis that catalyses the interconversion of glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). Glucose-6-Phosphate 116-135 glucose-6-phosphate isomerase Homo sapiens 26-29 20208175-1 2010 Phosphoglucose isomerase (PGI) is a key enzyme in glycolysis and glycogenesis that catalyses the interconversion of glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). Glucose-6-Phosphate 137-140 glucose-6-phosphate isomerase Homo sapiens 26-29 20208175-1 2010 Phosphoglucose isomerase (PGI) is a key enzyme in glycolysis and glycogenesis that catalyses the interconversion of glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). fructose-6-phosphate 146-166 glucose-6-phosphate isomerase Homo sapiens 26-29 20208175-1 2010 Phosphoglucose isomerase (PGI) is a key enzyme in glycolysis and glycogenesis that catalyses the interconversion of glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). fructose-6-phosphate 168-171 glucose-6-phosphate isomerase Homo sapiens 26-29 20129665-6 2010 In placental villi from female babies aldosterone significantly increased the rate of recovery of pH(i) from an acid load (0.0087 +/- 0.0005 versus 0.0056 +/- 0.0009 pH units/s, n = 8 p < 0.05 Paired Student"s t-test) which was inhibited by the mineralocorticoid receptor antagonist, spironolactone (1 microM) but not the glucocorticoid antagonist mifepristone (1 microM). Spironolactone 287-301 glucose-6-phosphate isomerase Homo sapiens 98-103 20129665-6 2010 In placental villi from female babies aldosterone significantly increased the rate of recovery of pH(i) from an acid load (0.0087 +/- 0.0005 versus 0.0056 +/- 0.0009 pH units/s, n = 8 p < 0.05 Paired Student"s t-test) which was inhibited by the mineralocorticoid receptor antagonist, spironolactone (1 microM) but not the glucocorticoid antagonist mifepristone (1 microM). Mifepristone 351-363 glucose-6-phosphate isomerase Homo sapiens 98-103 20174466-5 2010 Participants taking ASA alone had reduced urine Tx-M/PGI-M compared to no ASA or NSAID; however, participants taking NSAIDs plus ASA did not have reduced urine Tx-M/PGI-M ratio compared to NSAIDs alone. Aspirin 20-23 glucose-6-phosphate isomerase Homo sapiens 53-56 19672867-1 2010 BACKGROUND: In this study, we tested the ability of a novel poly(adenosine diphosphate ribose) polymerase (PARP) inhibitor, 10-(4-methyl-piperazin-1-ylmethyl)-2H-7-oxa-1,2-diaza-benzo[de]-anthracen-3-one (GPI-15427), to enhance the effect of radiotherapy in a xenograft model of human head and neck squamous cell carcinoma (HNSCC). 10-(4-methyl-piperazin-1-ylmethyl)-2h-7-oxa-1,2-diaza-benzo[de]-anthracen-3-one 124-203 glucose-6-phosphate isomerase Homo sapiens 205-208 19815513-2 2009 GPI is synthesized in the endoplasmic reticulum (ER) from phosphatidylinositol (PI) through step-wise reactions including transfers of monosaccharides and preassembled GPI is transferred en bloc to proteins. Phosphatidylinositols 58-78 glucose-6-phosphate isomerase Homo sapiens 0-3 19903819-0 2010 Isolation of novel animal cell lines defective in glycerolipid biosynthesis reveals mutations in glucose-6-phosphate isomerase. glycerolipid 50-62 glucose-6-phosphate isomerase Homo sapiens 97-126 19903819-5 2010 Expression cloning and sequencing of the cDNA obtained from GroD1 revealed a point mutation, Gly-189 --> Glu, in glucose-6-phosphate isomerase (GPI), a glycolytic enzyme involved in an inherited disorder that results in anemia and neuromuscular symptoms in humans. Glycine 93-96 glucose-6-phosphate isomerase Homo sapiens 116-145 19903819-5 2010 Expression cloning and sequencing of the cDNA obtained from GroD1 revealed a point mutation, Gly-189 --> Glu, in glucose-6-phosphate isomerase (GPI), a glycolytic enzyme involved in an inherited disorder that results in anemia and neuromuscular symptoms in humans. Glycine 93-96 glucose-6-phosphate isomerase Homo sapiens 147-150 19903819-5 2010 Expression cloning and sequencing of the cDNA obtained from GroD1 revealed a point mutation, Gly-189 --> Glu, in glucose-6-phosphate isomerase (GPI), a glycolytic enzyme involved in an inherited disorder that results in anemia and neuromuscular symptoms in humans. Glutamic Acid 108-111 glucose-6-phosphate isomerase Homo sapiens 116-145 19903819-5 2010 Expression cloning and sequencing of the cDNA obtained from GroD1 revealed a point mutation, Gly-189 --> Glu, in glucose-6-phosphate isomerase (GPI), a glycolytic enzyme involved in an inherited disorder that results in anemia and neuromuscular symptoms in humans. Glutamic Acid 108-111 glucose-6-phosphate isomerase Homo sapiens 147-150 19903819-8 2010 Expression of wild-type hamster GPI restored GPI activity, glycerolipid biosynthesis, and PAP1 activity in GroD1. glycerolipid 59-71 glucose-6-phosphate isomerase Homo sapiens 32-35 19903819-10 2010 These findings uncover a novel relationship between GPI, involved in carbohydrate metabolism, and PAP1, a lipogenic enzyme. Carbohydrates 69-81 glucose-6-phosphate isomerase Homo sapiens 52-55 19837660-6 2009 Acidic pH(i) potentiated the MgATP dependence of wild-type CFTR by increasing MgATP affinity and enhancing channel activity, whereas alkaline pH(i) inhibited the MgATP dependence of wild-type CFTR by decreasing channel activity. Adenosine Triphosphate 29-34 glucose-6-phosphate isomerase Homo sapiens 7-12 19837660-6 2009 Acidic pH(i) potentiated the MgATP dependence of wild-type CFTR by increasing MgATP affinity and enhancing channel activity, whereas alkaline pH(i) inhibited the MgATP dependence of wild-type CFTR by decreasing channel activity. Adenosine Triphosphate 78-83 glucose-6-phosphate isomerase Homo sapiens 7-12 19837660-6 2009 Acidic pH(i) potentiated the MgATP dependence of wild-type CFTR by increasing MgATP affinity and enhancing channel activity, whereas alkaline pH(i) inhibited the MgATP dependence of wild-type CFTR by decreasing channel activity. Adenosine Triphosphate 78-83 glucose-6-phosphate isomerase Homo sapiens 7-12 19837660-7 2009 Because these data suggest that pH(i) modulates the interaction of MgATP with the nucleotide-binding domains (NBDs) of CFTR, we examined the pH(i) dependence of site-directed mutations in the two ATP-binding sites of CFTR that are located at the NBD1:NBD2 dimer interface (site 1: K464A-, D572N-, and G1349D-CFTR; site 2: G551D-, K1250M-, and D1370N-CFTR). Adenosine Triphosphate 67-72 glucose-6-phosphate isomerase Homo sapiens 32-37 19837660-7 2009 Because these data suggest that pH(i) modulates the interaction of MgATP with the nucleotide-binding domains (NBDs) of CFTR, we examined the pH(i) dependence of site-directed mutations in the two ATP-binding sites of CFTR that are located at the NBD1:NBD2 dimer interface (site 1: K464A-, D572N-, and G1349D-CFTR; site 2: G551D-, K1250M-, and D1370N-CFTR). Adenosine Triphosphate 69-72 glucose-6-phosphate isomerase Homo sapiens 32-37 19837660-9 2009 The effects of pH(i) also suggest that site 2 might employ substrate-assisted catalysis to ensure that ATP hydrolysis follows NBD dimerization. Adenosine Triphosphate 103-106 glucose-6-phosphate isomerase Homo sapiens 15-20 20376224-15 2009 The proposed formula for assay on anhydrous basis= (assay on as-is basisxPhi)/(Phi-%water) in which Phi is sum of experimental results of assay and water content tests experimentally determined, separately, on as-is basis. Water 84-89 glucose-6-phosphate isomerase Homo sapiens 79-82 20376224-15 2009 The proposed formula for assay on anhydrous basis= (assay on as-is basisxPhi)/(Phi-%water) in which Phi is sum of experimental results of assay and water content tests experimentally determined, separately, on as-is basis. Water 148-153 glucose-6-phosphate isomerase Homo sapiens 79-82 19745779-5 2009 Intracellular pH (pHi) was measured by microfluorometry in cells loaded with 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein 77-126 glucose-6-phosphate isomerase Homo sapiens 18-21 19745779-5 2009 Intracellular pH (pHi) was measured by microfluorometry in cells loaded with 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). bcecf 128-133 glucose-6-phosphate isomerase Homo sapiens 18-21 19745779-6 2009 RESULTS: In HCO3(-)-free solutions, ATP-evoked changes in short-circuit current were inhibited by bumetanide, and the recovery of pHi from acid loading was abolished by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA). Bicarbonates 12-16 glucose-6-phosphate isomerase Homo sapiens 130-133 19745779-6 2009 RESULTS: In HCO3(-)-free solutions, ATP-evoked changes in short-circuit current were inhibited by bumetanide, and the recovery of pHi from acid loading was abolished by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA). Adenosine Triphosphate 36-39 glucose-6-phosphate isomerase Homo sapiens 130-133 19745779-6 2009 RESULTS: In HCO3(-)-free solutions, ATP-evoked changes in short-circuit current were inhibited by bumetanide, and the recovery of pHi from acid loading was abolished by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA). ethylisopropylamiloride 169-202 glucose-6-phosphate isomerase Homo sapiens 130-133 19745779-6 2009 RESULTS: In HCO3(-)-free solutions, ATP-evoked changes in short-circuit current were inhibited by bumetanide, and the recovery of pHi from acid loading was abolished by 5-(N-ethyl-N-isopropyl)-amiloride (EIPA). ethylisopropylamiloride 204-208 glucose-6-phosphate isomerase Homo sapiens 130-133 19752774-11 2009 CONCLUSIONS: Bile salts modulate pHi, [Ca(2+)]i, and apical anion exchange activity in human pancreatic duct cells. Bile Acids and Salts 13-23 glucose-6-phosphate isomerase Homo sapiens 33-36 20380799-0 2010 [Phenylhexyl isothiocyanate (PHI) regulates histone methylation and acetylation and induces apoptosis in SMMC-7721 cells]. 6-phenylhexyl isothiocyanate 1-27 glucose-6-phosphate isomerase Homo sapiens 29-32 20380799-5 2010 PHI treatment resulted in increased acetylation of histone H3 and H4 , elevated level of histone H3 lysine 4 methylation, and decreased level of histone H3 lysine 9 methylation. Lysine 100-106 glucose-6-phosphate isomerase Homo sapiens 0-3 20380799-5 2010 PHI treatment resulted in increased acetylation of histone H3 and H4 , elevated level of histone H3 lysine 4 methylation, and decreased level of histone H3 lysine 9 methylation. Lysine 156-162 glucose-6-phosphate isomerase Homo sapiens 0-3 20015293-4 2010 As moclobemide, an antidepressant, and also some antiepileptic drugs can reduce neuronal pHi in hippocampus slices in vitro, we screened a panel of currently used neuropsychopharmaca for comparable effects. Moclobemide 3-14 glucose-6-phosphate isomerase Homo sapiens 89-92 19815513-2 2009 GPI is synthesized in the endoplasmic reticulum (ER) from phosphatidylinositol (PI) through step-wise reactions including transfers of monosaccharides and preassembled GPI is transferred en bloc to proteins. Phosphatidylinositols 58-78 glucose-6-phosphate isomerase Homo sapiens 168-171 19815513-2 2009 GPI is synthesized in the endoplasmic reticulum (ER) from phosphatidylinositol (PI) through step-wise reactions including transfers of monosaccharides and preassembled GPI is transferred en bloc to proteins. Monosaccharides 135-150 glucose-6-phosphate isomerase Homo sapiens 0-3 19815513-3 2009 Cellular PI contains mostly diacyl glycerol and unsaturated fatty acid in the sn-2 position, whereas mammalian GPI-APs have mainly 1-alkyl-2-acyl PI and almost exclusively stearic acid, a saturated chain, at the sn-2 position. stearic acid 172-184 glucose-6-phosphate isomerase Homo sapiens 111-114 19815513-4 2009 The latter characteristic is the result of fatty acid remodeling occurring in the Golgi, generating GPI-anchors compatible with raft membrane. Fatty Acids 43-53 glucose-6-phosphate isomerase Homo sapiens 100-103 19815513-5 2009 The former characteristic is the result of diacyl to alkyl-acyl change occurring in the third GPI intermediate, glucosaminyl-inositolacylated-PI (GlcN-acyl-PI). glucosaminyl-inositolacylated-pi 112-144 glucose-6-phosphate isomerase Homo sapiens 94-97 19815513-7 2009 Using cell lines defective in the peroxisomal alkyl-phospholipid biosynthetic pathway, we demonstrated that generation of alkyl-containing GPI is dependent upon the peroxisomal pathway. alkyl-phospholipid 46-64 glucose-6-phosphate isomerase Homo sapiens 139-142 19774243-1 2009 A reactive ten-membered ring enyne-allene (tau(25 degrees C) = 5-6 min) is efficiently generated (Phi(300 nm) = 0.57) by UV irradiation of a thermally stable precursor in which a triple bond is masked as a cyclopropenone moiety. enyne-allene 29-41 glucose-6-phosphate isomerase Homo sapiens 98-101 19774243-1 2009 A reactive ten-membered ring enyne-allene (tau(25 degrees C) = 5-6 min) is efficiently generated (Phi(300 nm) = 0.57) by UV irradiation of a thermally stable precursor in which a triple bond is masked as a cyclopropenone moiety. cyclopropenone 206-220 glucose-6-phosphate isomerase Homo sapiens 98-101 19778729-4 2009 Inspired by this design, we have produced recombinant hexokinase type 1 and glucose-6-phosphate isomerase capable of oriented immobilization on a nickel-nitrilotriacetic acid modified surface. nickel nitrilotriacetic acid 146-174 glucose-6-phosphate isomerase Homo sapiens 54-105 19137453-0 2009 Antibodies to glycosylphosphatidyl-inositol anchored proteins (GPI-AP) in antithymocyte and antilymphocyte globulin: possible role for the expansion of GPI-AP deficient cells in aplastic anemia. Glycosylphosphatidylinositols 14-43 glucose-6-phosphate isomerase Homo sapiens 63-66 19137453-0 2009 Antibodies to glycosylphosphatidyl-inositol anchored proteins (GPI-AP) in antithymocyte and antilymphocyte globulin: possible role for the expansion of GPI-AP deficient cells in aplastic anemia. Glycosylphosphatidylinositols 14-43 glucose-6-phosphate isomerase Homo sapiens 152-155 19137453-2 2009 In this study we have investigated whether commercial ATG/ALG preparations contain antibodies against glycosylphosphatidyl-inositol anchored proteins (GPI-AP), which could be responsible for emergence of GPI-deficient populations in aplastic anemia after ATG/ALG therapy. Glycosylphosphatidylinositols 102-131 glucose-6-phosphate isomerase Homo sapiens 151-154 19137453-2 2009 In this study we have investigated whether commercial ATG/ALG preparations contain antibodies against glycosylphosphatidyl-inositol anchored proteins (GPI-AP), which could be responsible for emergence of GPI-deficient populations in aplastic anemia after ATG/ALG therapy. Glycosylphosphatidylinositols 102-131 glucose-6-phosphate isomerase Homo sapiens 204-207 19112160-4 2009 Phosphorus-31 magnetic resonance spectroscopy ((31)P-MRS) was used to noninvasively monitor the intracellular concentrations of phosphocreatine ([PCr]) and inorganic phosphate ([P(i)]) as well as intracellular pH (pH(i)) status during exercise in WRM and Con. Phosphorus 0-10 glucose-6-phosphate isomerase Homo sapiens 214-219 19954620-8 2009 The accumulation of Rh123 was 71.03+/-0.47 at pHi 7.0, which was increased obviously as compared to the control group 20.07+/-0.39. Rhodamine 123 20-25 glucose-6-phosphate isomerase Homo sapiens 46-49 19349276-7 2009 Clustering of TMD-HA-YFP with GPI-CFP was observed and shown to be reduced upon cholesterol depletion, a treatment known to disrupt rafts. Cholesterol 80-91 glucose-6-phosphate isomerase Homo sapiens 30-33 19583255-1 2009 It is demonstrated that sortase A (SrtA) can catalyze efficient coupling of peptides to GPI analogues with a glycine residue attached to the phosphoethanolamine moiety at the nonreducing end to form GPI-linked peptides. Glycine 109-116 glucose-6-phosphate isomerase Homo sapiens 199-202 19583255-1 2009 It is demonstrated that sortase A (SrtA) can catalyze efficient coupling of peptides to GPI analogues with a glycine residue attached to the phosphoethanolamine moiety at the nonreducing end to form GPI-linked peptides. phosphorylethanolamine 141-160 glucose-6-phosphate isomerase Homo sapiens 88-91 19583255-1 2009 It is demonstrated that sortase A (SrtA) can catalyze efficient coupling of peptides to GPI analogues with a glycine residue attached to the phosphoethanolamine moiety at the nonreducing end to form GPI-linked peptides. phosphorylethanolamine 141-160 glucose-6-phosphate isomerase Homo sapiens 199-202 19522522-1 2009 Quantum yields, Phi, for the production of the formyl radical, HCO, in the photolysis of glyoxal were determined at 85 wavelengths, lambda, in the range of 290-420 nm at pressures between 50 and 550 Torr (N(2)) at 298 K using pulsed-laser photolysis combined with cavity ring-down spectroscopy detection of HCO. formyl phosphate 47-61 glucose-6-phosphate isomerase Homo sapiens 16-19 19522522-1 2009 Quantum yields, Phi, for the production of the formyl radical, HCO, in the photolysis of glyoxal were determined at 85 wavelengths, lambda, in the range of 290-420 nm at pressures between 50 and 550 Torr (N(2)) at 298 K using pulsed-laser photolysis combined with cavity ring-down spectroscopy detection of HCO. 7 alpha-hydroxy-4-cholesten-3-one 63-66 glucose-6-phosphate isomerase Homo sapiens 16-19 19522522-1 2009 Quantum yields, Phi, for the production of the formyl radical, HCO, in the photolysis of glyoxal were determined at 85 wavelengths, lambda, in the range of 290-420 nm at pressures between 50 and 550 Torr (N(2)) at 298 K using pulsed-laser photolysis combined with cavity ring-down spectroscopy detection of HCO. Glyoxal 89-96 glucose-6-phosphate isomerase Homo sapiens 16-19 19708109-4 2009 Upon exposure to the saturated vapor of cyclohexane, which has preferential affinity for the PB block, a "threshold" of Phi(PS) (approximate 0.635-0.707) was found. Cyclohexane 40-51 glucose-6-phosphate isomerase Homo sapiens 120-127 19195490-4 2009 We also designed a chimera that had an additional N-terminal TPA leader sequence (pTPA.GPI-PA63) with an aim to target GPI-PA63 to ER where new CD1 molecules are synthesized. Tetradecanoylphorbol Acetate 61-64 glucose-6-phosphate isomerase Homo sapiens 87-90 19028925-6 2009 pHi then recovered by a mechanism that was shown to be particularly sensitive to the presence of the inhibitor DIDS (4,4"-diisothiocyanostilbene disulfonic acid). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 111-115 glucose-6-phosphate isomerase Homo sapiens 0-3 19064002-1 2009 Glucose-6-phosphate isomerase (GPI), a homodimeric enzyme, catalyzes the interconversion between glucose-6-phosphate and fructose-6-phosphate. Glucose-6-Phosphate 97-116 glucose-6-phosphate isomerase Homo sapiens 0-29 19064002-1 2009 Glucose-6-phosphate isomerase (GPI), a homodimeric enzyme, catalyzes the interconversion between glucose-6-phosphate and fructose-6-phosphate. Glucose-6-Phosphate 97-116 glucose-6-phosphate isomerase Homo sapiens 31-34 19064002-1 2009 Glucose-6-phosphate isomerase (GPI), a homodimeric enzyme, catalyzes the interconversion between glucose-6-phosphate and fructose-6-phosphate. fructose-6-phosphate 121-141 glucose-6-phosphate isomerase Homo sapiens 0-29 19064002-1 2009 Glucose-6-phosphate isomerase (GPI), a homodimeric enzyme, catalyzes the interconversion between glucose-6-phosphate and fructose-6-phosphate. fructose-6-phosphate 121-141 glucose-6-phosphate isomerase Homo sapiens 31-34 19028925-6 2009 pHi then recovered by a mechanism that was shown to be particularly sensitive to the presence of the inhibitor DIDS (4,4"-diisothiocyanostilbene disulfonic acid). 4,4"-diisothiocyanostilbene disulfonic acid 117-160 glucose-6-phosphate isomerase Homo sapiens 0-3 19028925-7 2009 In the presence of amiloride and PSA (picrylsulfonic acid), pHi recovery was also significantly affected. Amiloride 19-28 glucose-6-phosphate isomerase Homo sapiens 60-63 19028925-7 2009 In the presence of amiloride and PSA (picrylsulfonic acid), pHi recovery was also significantly affected. Trinitrobenzenesulfonic Acid 33-36 glucose-6-phosphate isomerase Homo sapiens 60-63 19028925-7 2009 In the presence of amiloride and PSA (picrylsulfonic acid), pHi recovery was also significantly affected. Trinitrobenzenesulfonic Acid 38-57 glucose-6-phosphate isomerase Homo sapiens 60-63 19028925-8 2009 These results indicate that, in the experimental conditions used, pHi is regulated by the action of an Na(+)-driven HCO(3)(-)/Cl(-) exchanger and an Na(+)/HCO(3)(-) co-transporter and also by the action of the Na(+)/H(+) exchanger. 7 alpha-hydroxy-4-cholesten-3-one 116-119 glucose-6-phosphate isomerase Homo sapiens 66-69 19028925-10 2009 Finally, pHi was shown to be sensitive to the removal of external Cl(-), but not of Na(+) or K(+), evidencing the presence of a membrane Cl(-)-dependent base extruder, namely the Na(+)-independent HCO(3)(-)/Cl(-) exchanger, and its role on pHi maintenance on these cells. 7 alpha-hydroxy-4-cholesten-3-one 197-201 glucose-6-phosphate isomerase Homo sapiens 9-12 19255516-9 2009 The decrease of pHi and of DeltapHi following NS1 expression could be significantly blunted by inhibition of caspase 3 with zVAD. benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone 124-128 glucose-6-phosphate isomerase Homo sapiens 16-19 19155602-2 2009 Studies using (31)-phosphorus magnetic resonance spectroscopy ((31)P-MRS) have shown that phosphocreatine (PCr) and muscle pH (pHi) are significantly decreased in patients with COPD during mild exercise, suggesting the early activation of anaerobic glycolysis in their muscles. Phosphorus 19-29 glucose-6-phosphate isomerase Homo sapiens 127-130 19017628-7 2009 The Phi(PSII) values of Boston fern chloroplasts decreased in the order mesophyll, epidermal and guard cell chloroplasts. mesophyll 72-81 glucose-6-phosphate isomerase Homo sapiens 8-12 18561188-0 2009 Crystal structure of phosphoglucose isomerase from Trypanosoma brucei complexed with glucose-6-phosphate at 1.6 A resolution. Glucose-6-Phosphate 85-104 glucose-6-phosphate isomerase Homo sapiens 21-45 19053540-5 2008 In particular, phi for Ca(NO(3))(2) was found to have a maximum value of (7.8 +/- 0.1) x 10(-3) for nitrate ion solution concentrations near one molal, with the smallest quantum yield for the highest concentration solution above 14 m nitrate ion, phi = (2.3 +/- 2.0) x 10(-4). Nitrates 100-107 glucose-6-phosphate isomerase Homo sapiens 15-18 19053540-5 2008 In particular, phi for Ca(NO(3))(2) was found to have a maximum value of (7.8 +/- 0.1) x 10(-3) for nitrate ion solution concentrations near one molal, with the smallest quantum yield for the highest concentration solution above 14 m nitrate ion, phi = (2.3 +/- 2.0) x 10(-4). Nitrates 100-107 glucose-6-phosphate isomerase Homo sapiens 247-250 19053540-5 2008 In particular, phi for Ca(NO(3))(2) was found to have a maximum value of (7.8 +/- 0.1) x 10(-3) for nitrate ion solution concentrations near one molal, with the smallest quantum yield for the highest concentration solution above 14 m nitrate ion, phi = (2.3 +/- 2.0) x 10(-4). Nitrates 234-241 glucose-6-phosphate isomerase Homo sapiens 15-18 19053540-5 2008 In particular, phi for Ca(NO(3))(2) was found to have a maximum value of (7.8 +/- 0.1) x 10(-3) for nitrate ion solution concentrations near one molal, with the smallest quantum yield for the highest concentration solution above 14 m nitrate ion, phi = (2.3 +/- 2.0) x 10(-4). Nitrates 234-241 glucose-6-phosphate isomerase Homo sapiens 247-250 19053540-4 2008 In particular, the quantum yield (phi) of nitrite formation was measured and found to significantly decrease at high concentrations of nitrate for Ca(NO(3))(2). Nitrites 42-49 glucose-6-phosphate isomerase Homo sapiens 34-37 19053540-4 2008 In particular, the quantum yield (phi) of nitrite formation was measured and found to significantly decrease at high concentrations of nitrate for Ca(NO(3))(2). Nitrates 135-142 glucose-6-phosphate isomerase Homo sapiens 34-37 19053540-4 2008 In particular, the quantum yield (phi) of nitrite formation was measured and found to significantly decrease at high concentrations of nitrate for Ca(NO(3))(2). punky blue 150-156 glucose-6-phosphate isomerase Homo sapiens 34-37 19155602-9 2009 These findings might reflect a slower increase in O(2) delivery in patients with a low pHi, which might partly account for altered muscle energy metabolism. o(2) 50-54 glucose-6-phosphate isomerase Homo sapiens 87-90 19032829-5 2008 Of the 20 patients with anti-GPI Abs, 17 (85%) were positive for anti-CCP Abs. cyclic citrullinated peptide 70-73 glucose-6-phosphate isomerase Homo sapiens 29-32 18515784-3 2008 Dilated lipotoxic cardiomyopathy, thought to be the result of diabetes and severe obesity, has been modeled in several genetically altered mice, including animals with cardiac-specific overexpression of glycosylphosphatidylinositol (GPI)-anchored human lipoprotein lipase (LpL(GPI)). Glycosylphosphatidylinositols 203-231 glucose-6-phosphate isomerase Homo sapiens 233-236 19053540-6 2008 The effect of the addition of the radical scavenger, formate, on the 310 nm photolysis of these solutions was also investigated and found to increase phi by a factor of 2 or more for both sodium and calcium nitrate solutions. formic acid 53-60 glucose-6-phosphate isomerase Homo sapiens 150-153 19053540-6 2008 The effect of the addition of the radical scavenger, formate, on the 310 nm photolysis of these solutions was also investigated and found to increase phi by a factor of 2 or more for both sodium and calcium nitrate solutions. Sodium 188-194 glucose-6-phosphate isomerase Homo sapiens 150-153 19053540-6 2008 The effect of the addition of the radical scavenger, formate, on the 310 nm photolysis of these solutions was also investigated and found to increase phi by a factor of 2 or more for both sodium and calcium nitrate solutions. calcium nitrate 199-214 glucose-6-phosphate isomerase Homo sapiens 150-153 19053540-7 2008 In the presence of formate, Ca(NO(3))(2) solutions again showed a significant decrease in phi with increasing NO(3)(-) concentration: phi = (1.4 +/- 0.1) x 10(-2) at (1.0 +/- 0.1) x 10(-2) m NO(3)(-) compared to phi = (4.2 +/- 0.3) x 10(-3) at 14.9 +/- 0.1 m NO(3)(-). formic acid 19-26 glucose-6-phosphate isomerase Homo sapiens 90-93 19053540-7 2008 In the presence of formate, Ca(NO(3))(2) solutions again showed a significant decrease in phi with increasing NO(3)(-) concentration: phi = (1.4 +/- 0.1) x 10(-2) at (1.0 +/- 0.1) x 10(-2) m NO(3)(-) compared to phi = (4.2 +/- 0.3) x 10(-3) at 14.9 +/- 0.1 m NO(3)(-). formic acid 19-26 glucose-6-phosphate isomerase Homo sapiens 134-137 19053540-7 2008 In the presence of formate, Ca(NO(3))(2) solutions again showed a significant decrease in phi with increasing NO(3)(-) concentration: phi = (1.4 +/- 0.1) x 10(-2) at (1.0 +/- 0.1) x 10(-2) m NO(3)(-) compared to phi = (4.2 +/- 0.3) x 10(-3) at 14.9 +/- 0.1 m NO(3)(-). formic acid 19-26 glucose-6-phosphate isomerase Homo sapiens 134-137 18778408-1 2008 Oligomerization of glycosyl-phosphatidylinositol-anchored proteins (GPI-APs) into high molecular weight complexes is an essential step for their apical sorting in polarized epithelial cells. Glycosylphosphatidylinositols 19-48 glucose-6-phosphate isomerase Homo sapiens 68-71 18778408-4 2008 We found that both the N- and O-glycosylation mutants are apically sorted, associate to detergent-resistant microdomains and are able to oligomerize, like the wild-type proteins, suggesting that glycosylation does not have a direct role in GPI-AP oligomerization and apical sorting. Nitrogen 23-24 glucose-6-phosphate isomerase Homo sapiens 240-243 18506424-9 2008 The pharmacological relevance of this effect is supported by literature data suggesting brain acidosis in bipolar patients and by preliminary observations that carbamazepine and valproate also increase pHi in astrocytes. Carbamazepine 160-173 glucose-6-phosphate isomerase Homo sapiens 202-205 18506424-9 2008 The pharmacological relevance of this effect is supported by literature data suggesting brain acidosis in bipolar patients and by preliminary observations that carbamazepine and valproate also increase pHi in astrocytes. Valproic Acid 178-187 glucose-6-phosphate isomerase Homo sapiens 202-205 18789114-3 2008 The other was to determine whether direct hemoperfusion with a polymyxin B fiber column (DHP-PMX) could improve the pHi if it remained low after early goal-directed therapy. dhp-pmx 89-96 glucose-6-phosphate isomerase Homo sapiens 116-119 18789114-6 2008 The pHi was 7.22 +/- 0.04 immediately before DHP-PMX, 7.28 +/- 0.03 (P < 0.05) at 24 h, 7.32 +/- 0.03 (P < 0.01) at 48 h, and 7.34 +/- 0.02 (P < 0.01) at 72 h, showing a significant increase from 24 h onward compared with the pretreatment value. dhp-pmx 45-52 glucose-6-phosphate isomerase Homo sapiens 4-7 18789114-7 2008 In patients with sepsis and septic shock, the pHi remained low after early goal-directed therapy; however, it was significantly improved from 24 h after the start of DHP-PMX and was normalized from 48 h onwards. dhp-pmx 166-173 glucose-6-phosphate isomerase Homo sapiens 46-49 18789114-8 2008 These findings suggest that DHP-PMX improves pHi. dhp-pmx 28-35 glucose-6-phosphate isomerase Homo sapiens 45-48 18544084-5 2008 When methyl-beta-cyclodextrin (MbetaCD) was utilized to probe for functional differences between normal and GPI(-) LR, increased levels of phospho-p38 mitogen-activated protein kinase (MAPK), and phospho-p65 nuclear factor NF-kappaB were found in control and GPI(-) cells respectively. methyl-beta-cyclodextrin 5-29 glucose-6-phosphate isomerase Homo sapiens 108-111 18544084-5 2008 When methyl-beta-cyclodextrin (MbetaCD) was utilized to probe for functional differences between normal and GPI(-) LR, increased levels of phospho-p38 mitogen-activated protein kinase (MAPK), and phospho-p65 nuclear factor NF-kappaB were found in control and GPI(-) cells respectively. methyl-beta-cyclodextrin 5-29 glucose-6-phosphate isomerase Homo sapiens 259-262 18544084-5 2008 When methyl-beta-cyclodextrin (MbetaCD) was utilized to probe for functional differences between normal and GPI(-) LR, increased levels of phospho-p38 mitogen-activated protein kinase (MAPK), and phospho-p65 nuclear factor NF-kappaB were found in control and GPI(-) cells respectively. methyl-beta-cyclodextrin 31-38 glucose-6-phosphate isomerase Homo sapiens 108-111 18544084-5 2008 When methyl-beta-cyclodextrin (MbetaCD) was utilized to probe for functional differences between normal and GPI(-) LR, increased levels of phospho-p38 mitogen-activated protein kinase (MAPK), and phospho-p65 nuclear factor NF-kappaB were found in control and GPI(-) cells respectively. methyl-beta-cyclodextrin 31-38 glucose-6-phosphate isomerase Homo sapiens 259-262 18544084-6 2008 Subsequent experiments addressing the inhibition of phosphoinositide-3-kinase (PI3K) suggest that the PI3K/AKT pathway may be responsible for the resistance of K562 GPI(-)cells to negative effects of MbetaCD. methyl-beta-cyclodextrin 200-207 glucose-6-phosphate isomerase Homo sapiens 165-168 18298402-10 2008 Due to this characteristic, G(L)-GPi in comparison with GPis could offer an advantageous risk/benefit profile circumventing the potential downsides of a complete prevention of glycogen breakdown while retaining glucose-lowering efficacy, suggesting that inhibition of the G(L)-GP interaction may provide an attractive novel approach for rebalancing the disturbed glycogen metabolism in diabetic patients. Glycogen 176-184 glucose-6-phosphate isomerase Homo sapiens 33-36 18065787-9 2008 CONCLUSIONS: HD patients with a low P(HCO3) exhibited low neutrophil pHi that in turn increased the expression of CD11b/CD18 compared with neutrophils with a normal or high pHi. Bicarbonates 38-42 glucose-6-phosphate isomerase Homo sapiens 69-72 18416535-1 2008 The influence of membrane lipid environment on the activity of GPI-anchored enzymes was investigated with human placental alkaline phosphatase reconstituted by a detergent-dialysis technique in liposomes composed of palmitoyloleoylphosphatidylcholine, alone or in mixture with lipids enriched along with the protein within lipid rafts: cholesterol, sphingomyelin, and GM1 ganglioside. 1-palmitoyl-2-oleoylphosphatidylcholine 216-250 glucose-6-phosphate isomerase Homo sapiens 63-66 18416535-1 2008 The influence of membrane lipid environment on the activity of GPI-anchored enzymes was investigated with human placental alkaline phosphatase reconstituted by a detergent-dialysis technique in liposomes composed of palmitoyloleoylphosphatidylcholine, alone or in mixture with lipids enriched along with the protein within lipid rafts: cholesterol, sphingomyelin, and GM1 ganglioside. Cholesterol 336-347 glucose-6-phosphate isomerase Homo sapiens 63-66 18416535-1 2008 The influence of membrane lipid environment on the activity of GPI-anchored enzymes was investigated with human placental alkaline phosphatase reconstituted by a detergent-dialysis technique in liposomes composed of palmitoyloleoylphosphatidylcholine, alone or in mixture with lipids enriched along with the protein within lipid rafts: cholesterol, sphingomyelin, and GM1 ganglioside. Sphingomyelins 349-362 glucose-6-phosphate isomerase Homo sapiens 63-66 18416535-1 2008 The influence of membrane lipid environment on the activity of GPI-anchored enzymes was investigated with human placental alkaline phosphatase reconstituted by a detergent-dialysis technique in liposomes composed of palmitoyloleoylphosphatidylcholine, alone or in mixture with lipids enriched along with the protein within lipid rafts: cholesterol, sphingomyelin, and GM1 ganglioside. G(M1) Ganglioside 368-383 glucose-6-phosphate isomerase Homo sapiens 63-66 18416535-9 2008 These data open the possibility that the activity of GPI-anchored enzymes may be modulated by membrane microenvironment features, in particular by membrane curvature and cholesterol-enriched ordered microenvironments, such as those of lipid rafts. Cholesterol 170-181 glucose-6-phosphate isomerase Homo sapiens 53-56 20409900-4 2008 Monocytes were isolated, and intracellular pH (pHi) was measured by the use of Bis-(carboxyethyl)-5(6)-carboxy-fluorescein acetoxymethylester. bis-(carboxyethyl)-5(6)-carboxy-fluorescein acetoxymethylester 79-141 glucose-6-phosphate isomerase Homo sapiens 47-50 20409900-12 2008 Furthermore, angiotensin II caused an increase in pHi, which was reversed by cariporide in monocytes derived from hypertensive patients. cariporide 77-87 glucose-6-phosphate isomerase Homo sapiens 50-53 18065787-9 2008 CONCLUSIONS: HD patients with a low P(HCO3) exhibited low neutrophil pHi that in turn increased the expression of CD11b/CD18 compared with neutrophils with a normal or high pHi. Bicarbonates 38-42 glucose-6-phosphate isomerase Homo sapiens 173-176 18004228-1 2008 Mucosal pH (pHi) is influenced by local perfusion and metabolism (mucosal-arterial pCO2 gradient, DeltapCO2), systemic metabolic acidosis (arterial bicarbonate), and respiration (arterial pCO2). pco2 83-87 glucose-6-phosphate isomerase Homo sapiens 12-15 18425384-2 2008 When a phenoxazine derivative, 2-aminophenoxazine-3-one (Phx-3) or 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3H-phenoxazine-3-one (Phx-1) was added to these cells, pHi rapidly decreased within 20 min, dose-dependently, though the extent of the decrease of pHi was significantly larger for Phx-3 (a decrease of 0.9 units) than for Phx-1 (a decrease of 0.4 units). phenoxazine 7-18 glucose-6-phosphate isomerase Homo sapiens 165-168 18425384-2 2008 When a phenoxazine derivative, 2-aminophenoxazine-3-one (Phx-3) or 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3H-phenoxazine-3-one (Phx-1) was added to these cells, pHi rapidly decreased within 20 min, dose-dependently, though the extent of the decrease of pHi was significantly larger for Phx-3 (a decrease of 0.9 units) than for Phx-1 (a decrease of 0.4 units). phenoxazine 7-18 glucose-6-phosphate isomerase Homo sapiens 257-260 18425384-2 2008 When a phenoxazine derivative, 2-aminophenoxazine-3-one (Phx-3) or 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3H-phenoxazine-3-one (Phx-1) was added to these cells, pHi rapidly decreased within 20 min, dose-dependently, though the extent of the decrease of pHi was significantly larger for Phx-3 (a decrease of 0.9 units) than for Phx-1 (a decrease of 0.4 units). 3-aminophenoxazone 31-55 glucose-6-phosphate isomerase Homo sapiens 165-168 18425384-2 2008 When a phenoxazine derivative, 2-aminophenoxazine-3-one (Phx-3) or 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3H-phenoxazine-3-one (Phx-1) was added to these cells, pHi rapidly decreased within 20 min, dose-dependently, though the extent of the decrease of pHi was significantly larger for Phx-3 (a decrease of 0.9 units) than for Phx-1 (a decrease of 0.4 units). 3-aminophenoxazone 31-55 glucose-6-phosphate isomerase Homo sapiens 257-260 18425384-2 2008 When a phenoxazine derivative, 2-aminophenoxazine-3-one (Phx-3) or 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3H-phenoxazine-3-one (Phx-1) was added to these cells, pHi rapidly decreased within 20 min, dose-dependently, though the extent of the decrease of pHi was significantly larger for Phx-3 (a decrease of 0.9 units) than for Phx-1 (a decrease of 0.4 units). 2-amino-4,4alpha-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one 67-130 glucose-6-phosphate isomerase Homo sapiens 165-168 18425384-2 2008 When a phenoxazine derivative, 2-aminophenoxazine-3-one (Phx-3) or 2-amino-4,4alpha-dihydro-4alpha-7-dimethyl-3H-phenoxazine-3-one (Phx-1) was added to these cells, pHi rapidly decreased within 20 min, dose-dependently, though the extent of the decrease of pHi was significantly larger for Phx-3 (a decrease of 0.9 units) than for Phx-1 (a decrease of 0.4 units). 2-amino-4,4alpha-dihydro-4alpha,7-dimethyl-3H-phenoxazine-3-one 67-130 glucose-6-phosphate isomerase Homo sapiens 257-260 18004228-1 2008 Mucosal pH (pHi) is influenced by local perfusion and metabolism (mucosal-arterial pCO2 gradient, DeltapCO2), systemic metabolic acidosis (arterial bicarbonate), and respiration (arterial pCO2). deltapco2 98-107 glucose-6-phosphate isomerase Homo sapiens 12-15 18004228-1 2008 Mucosal pH (pHi) is influenced by local perfusion and metabolism (mucosal-arterial pCO2 gradient, DeltapCO2), systemic metabolic acidosis (arterial bicarbonate), and respiration (arterial pCO2). Bicarbonates 148-159 glucose-6-phosphate isomerase Homo sapiens 12-15 18004228-1 2008 Mucosal pH (pHi) is influenced by local perfusion and metabolism (mucosal-arterial pCO2 gradient, DeltapCO2), systemic metabolic acidosis (arterial bicarbonate), and respiration (arterial pCO2). pco2 103-107 glucose-6-phosphate isomerase Homo sapiens 12-15 18004228-11 2008 Arterial bicarbonate contributes more to pHi than the DeltapCO2 but is not associated with mortality. Bicarbonates 9-20 glucose-6-phosphate isomerase Homo sapiens 41-44 18661333-3 2008 This ubiquitously expressed sodium/hydrogen exchanger (NHE-1) plays a central housekeeping role in all cells regulating cell volume and internal pH (pHi). Sodium 28-34 glucose-6-phosphate isomerase Homo sapiens 149-152 18058122-1 2008 In plasma membranes, most glycosylphosphatidylinositol-anchored proteins (GPI proteins) would be associated with ordered microdomains enriched in sphingolipids and cholesterol. Sphingolipids 146-159 glucose-6-phosphate isomerase Homo sapiens 74-77 18058122-1 2008 In plasma membranes, most glycosylphosphatidylinositol-anchored proteins (GPI proteins) would be associated with ordered microdomains enriched in sphingolipids and cholesterol. Cholesterol 164-175 glucose-6-phosphate isomerase Homo sapiens 74-77 20641758-18 2004 (2) evaluated one of these analogs, 6-FPOL, and found that the molecule exhibited exceptional sensitivity to changes in pH and could be used to measure pHi and pHe simultaneously. 6-Fluoropyridoxol 36-42 glucose-6-phosphate isomerase Homo sapiens 152-155 18085290-0 2008 A mathematical model of pH(i) regulation in central CO2- chemoreception. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 52-55 glucose-6-phosphate isomerase Homo sapiens 24-29 18085290-1 2008 In CO2 chemosensitive neurons, an increase in CO2 (hypercapnia) leads to a maintained reduction in intracellular pH (pH(i)) while in non-chemosensitive neurons pH(i) recovery is observed. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 3-6 glucose-6-phosphate isomerase Homo sapiens 117-122 18085290-1 2008 In CO2 chemosensitive neurons, an increase in CO2 (hypercapnia) leads to a maintained reduction in intracellular pH (pH(i)) while in non-chemosensitive neurons pH(i) recovery is observed. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 46-49 glucose-6-phosphate isomerase Homo sapiens 117-122 18085290-1 2008 In CO2 chemosensitive neurons, an increase in CO2 (hypercapnia) leads to a maintained reduction in intracellular pH (pH(i)) while in non-chemosensitive neurons pH(i) recovery is observed. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 46-49 glucose-6-phosphate isomerase Homo sapiens 160-165 18085290-3 2008 Here, we compare the results of two different formulations of a mathematical model to begin to explore pH(i) regulation in central CO2 chemoreception. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 131-134 glucose-6-phosphate isomerase Homo sapiens 103-108 18068318-3 2008 The quantum yield for amlodipine base is Phi congruent with 0.001 under UV-A light, about one order of magnitude larger than that for the model bearing no amino group, supporting intramolecular assistance by that group. Amlodipine 22-32 glucose-6-phosphate isomerase Homo sapiens 41-44 18326903-6 2008 GPI-PLD activities were analyzed quantitatively by triton-X-114 partition with GPI anchored placental alkaline phosphatase (PLAP) as a substrate. Nonidet P-40 51-63 glucose-6-phosphate isomerase Homo sapiens 0-3 18991471-7 2008 At hours 2, 4 and 6, gastric pH in the pantoprazole, esomeprazole and rabeprazole groups increased significantly, whereas gastric juice volume decreased significantly, compared with the omeprazole and placebo groups (p < 0.001). Pantoprazole 39-51 glucose-6-phosphate isomerase Homo sapiens 29-31 18991471-7 2008 At hours 2, 4 and 6, gastric pH in the pantoprazole, esomeprazole and rabeprazole groups increased significantly, whereas gastric juice volume decreased significantly, compared with the omeprazole and placebo groups (p < 0.001). Rabeprazole 70-81 glucose-6-phosphate isomerase Homo sapiens 29-31 18991471-9 2008 CONCLUSION: This is the first study to show that single-dose pantoprazole, esomeprazole and rabeprazole are associated with greater gastric pH increase and greater gastric juice volume decrease than omeprazole in critically ill patients. Pantoprazole 61-73 glucose-6-phosphate isomerase Homo sapiens 140-142 18991471-9 2008 CONCLUSION: This is the first study to show that single-dose pantoprazole, esomeprazole and rabeprazole are associated with greater gastric pH increase and greater gastric juice volume decrease than omeprazole in critically ill patients. Esomeprazole 75-87 glucose-6-phosphate isomerase Homo sapiens 140-142 18991471-9 2008 CONCLUSION: This is the first study to show that single-dose pantoprazole, esomeprazole and rabeprazole are associated with greater gastric pH increase and greater gastric juice volume decrease than omeprazole in critically ill patients. Rabeprazole 92-103 glucose-6-phosphate isomerase Homo sapiens 140-142 18991471-9 2008 CONCLUSION: This is the first study to show that single-dose pantoprazole, esomeprazole and rabeprazole are associated with greater gastric pH increase and greater gastric juice volume decrease than omeprazole in critically ill patients. Omeprazole 77-87 glucose-6-phosphate isomerase Homo sapiens 140-142 18661333-3 2008 This ubiquitously expressed sodium/hydrogen exchanger (NHE-1) plays a central housekeeping role in all cells regulating cell volume and internal pH (pHi). Hydrogen 35-43 glucose-6-phosphate isomerase Homo sapiens 149-152 18661333-4 2008 At physiological pHi, NHE-1 is essentially inactive but it is extremely sensitive to pHi changes, being rapidly activated by small intracellular hydrogen concentration increases. Hydrogen 145-153 glucose-6-phosphate isomerase Homo sapiens 17-20 18661333-4 2008 At physiological pHi, NHE-1 is essentially inactive but it is extremely sensitive to pHi changes, being rapidly activated by small intracellular hydrogen concentration increases. Hydrogen 145-153 glucose-6-phosphate isomerase Homo sapiens 85-88 18097579-7 2008 Likewise, further analyses of the expression of related genes triggered by the D-(+)-glucose in vivo show that the activation process of these eight genes - GLUT1, HK1, GPI, GAPD, PGK1, PGK2, ENO2, PKM2 - prominently increased with a time-dependent effect. Glucose 79-92 glucose-6-phosphate isomerase Homo sapiens 169-172 17689202-5 2008 The long-chain unsaturated free fatty acids such as arachidonic acid (AA), PHi, pressure and temperature can increase the activity of TREK-2. unsaturated free fatty acids 15-43 glucose-6-phosphate isomerase Homo sapiens 75-78 18231737-6 2007 Platelet GP I a T allele was significantly associated with aspirin resistance as revealed by multiple logistic regression (OR=3.76, 95% CI: 2.87-9.58). Aspirin 59-66 glucose-6-phosphate isomerase Homo sapiens 9-13 18477915-10 2008 There was strong correlation between the abdominal rSO2 and pHi (r = .79; p < .0001) as well as between abdominal rSO2 and SVO2 (r = .89; p < .0001). rso2 51-55 glucose-6-phosphate isomerase Homo sapiens 60-63 18477915-13 2008 CONCLUSIONS: Abdominal site rSO2, measured in infants with either single or biventricular physiology, exhibits a strong correlation with gastric pHi as well as with serum lactate and SVO2. rso2 28-32 glucose-6-phosphate isomerase Homo sapiens 145-148 18974847-5 2008 We synthesized an AMF/PGI-paclitaxel conjugate and found it to be as efficient as free paclitaxel in inducing cytotoxicity and apoptosis in tumor cells that readily internalize AMF/PGI compared to tumor cells that poorly internalize AMF/PGI. Paclitaxel 26-36 glucose-6-phosphate isomerase Homo sapiens 22-25 18974847-5 2008 We synthesized an AMF/PGI-paclitaxel conjugate and found it to be as efficient as free paclitaxel in inducing cytotoxicity and apoptosis in tumor cells that readily internalize AMF/PGI compared to tumor cells that poorly internalize AMF/PGI. Paclitaxel 26-36 glucose-6-phosphate isomerase Homo sapiens 181-184 18974847-5 2008 We synthesized an AMF/PGI-paclitaxel conjugate and found it to be as efficient as free paclitaxel in inducing cytotoxicity and apoptosis in tumor cells that readily internalize AMF/PGI compared to tumor cells that poorly internalize AMF/PGI. Paclitaxel 26-36 glucose-6-phosphate isomerase Homo sapiens 181-184 18974847-7 2008 Moreover, following in vivo intratumoral injection, FITC-conjugated AMF/PGI is internalized in K1735-M1 tumors. Fluorescein-5-isothiocyanate 52-56 glucose-6-phosphate isomerase Homo sapiens 72-75 18974847-10 2008 Free AMF/PGI exhibited a pro-survival role, reducing the cytotoxic effect of both AMF/PGI-paclitaxel and free paclitaxel suggesting that AMF/PGI-paclitaxel targets a pathway associated with resistance to chemotherapeutic agents. Paclitaxel 90-100 glucose-6-phosphate isomerase Homo sapiens 9-12 18974847-10 2008 Free AMF/PGI exhibited a pro-survival role, reducing the cytotoxic effect of both AMF/PGI-paclitaxel and free paclitaxel suggesting that AMF/PGI-paclitaxel targets a pathway associated with resistance to chemotherapeutic agents. Paclitaxel 90-100 glucose-6-phosphate isomerase Homo sapiens 86-89 18974847-10 2008 Free AMF/PGI exhibited a pro-survival role, reducing the cytotoxic effect of both AMF/PGI-paclitaxel and free paclitaxel suggesting that AMF/PGI-paclitaxel targets a pathway associated with resistance to chemotherapeutic agents. Paclitaxel 90-100 glucose-6-phosphate isomerase Homo sapiens 86-89 18231737-7 2007 The results suggest that inherited platelet GP I a variations may have an important impact on aspirin resistance and the presence of GP I a T allele may be a marker of genetic susceptibility to aspirin resistance. Aspirin 94-101 glucose-6-phosphate isomerase Homo sapiens 44-48 18231737-7 2007 The results suggest that inherited platelet GP I a variations may have an important impact on aspirin resistance and the presence of GP I a T allele may be a marker of genetic susceptibility to aspirin resistance. Aspirin 194-201 glucose-6-phosphate isomerase Homo sapiens 133-137 17604179-9 2007 The quantum yield of oxygen uptake Phi(-O2) as a measure of formation of hydrogen peroxide increases with increasing amino acid concentration, approaching Phi(-O2) for AQ in air-saturated solution. Oxygen 21-27 glucose-6-phosphate isomerase Homo sapiens 35-38 18022597-6 2007 The pHi was measured with the pH-sensitive fluorescent dye bis-carboxyethyl carboxy-fluorescein (BCECF). bcecf 97-102 glucose-6-phosphate isomerase Homo sapiens 4-7 17690101-1 2007 Autocrine motility factor (AMF) is internalized via a receptor-mediated, dynamin-dependent, cholesterol-sensitive raft pathway to the smooth endoplasmic reticulum that is negatively regulated by caveolin-1. Cholesterol 92-103 glucose-6-phosphate isomerase Homo sapiens 0-25 17690101-1 2007 Autocrine motility factor (AMF) is internalized via a receptor-mediated, dynamin-dependent, cholesterol-sensitive raft pathway to the smooth endoplasmic reticulum that is negatively regulated by caveolin-1. Cholesterol 92-103 glucose-6-phosphate isomerase Homo sapiens 27-30 17690101-4 2007 AMF uptake, determined by FACS measurement of protease-insensitive internalized fluorescein-conjugated AMF, was increased in MCF7 and MDA-435 cells relative to MCF-10A and caveolin-1-expressing MDA-231 cells. Fluorescein 80-91 glucose-6-phosphate isomerase Homo sapiens 0-3 17690101-4 2007 AMF uptake, determined by FACS measurement of protease-insensitive internalized fluorescein-conjugated AMF, was increased in MCF7 and MDA-435 cells relative to MCF-10A and caveolin-1-expressing MDA-231 cells. Fluorescein 80-91 glucose-6-phosphate isomerase Homo sapiens 103-106 17690101-5 2007 Uptake of fluorescein-conjugated AMF was dynamin-dependent, methyl-beta-cyclodextrin- and genistein-sensitive, reduced upon overexpression of caveolin-1 in MDA-435 cells, and increased upon short hairpin RNA reduction of caveolin-1 in MDA-231 cells. Fluorescein 10-21 glucose-6-phosphate isomerase Homo sapiens 33-36 17690101-5 2007 Uptake of fluorescein-conjugated AMF was dynamin-dependent, methyl-beta-cyclodextrin- and genistein-sensitive, reduced upon overexpression of caveolin-1 in MDA-435 cells, and increased upon short hairpin RNA reduction of caveolin-1 in MDA-231 cells. methyl-beta-cyclodextrin 60-84 glucose-6-phosphate isomerase Homo sapiens 33-36 17690101-5 2007 Uptake of fluorescein-conjugated AMF was dynamin-dependent, methyl-beta-cyclodextrin- and genistein-sensitive, reduced upon overexpression of caveolin-1 in MDA-435 cells, and increased upon short hairpin RNA reduction of caveolin-1 in MDA-231 cells. Genistein 90-99 glucose-6-phosphate isomerase Homo sapiens 33-36 17690101-8 2007 AMF uptake in these cells was reduced by phosphatidylinositol 3-kinase inhibition but not by regulators of macropinocytosis such as amiloride, phorbol ester, or actin cytoskeleton disruption by cytochalasin D. Cytochalasin D 194-208 glucose-6-phosphate isomerase Homo sapiens 0-3 17690101-9 2007 The raft-dependent endocytosis of AMF therefore follows a distinct phosphatidylinositol 3-kinase-dependent pathway that is up-regulated in more aggressive tumor cells. Phosphatidylinositols 67-87 glucose-6-phosphate isomerase Homo sapiens 34-37 18022597-1 2007 The effect of Tris-Hydroxymethyl Aminomethane (THAM) on intracellular pH (pHi) is unknown. Tromethamine 47-51 glucose-6-phosphate isomerase Homo sapiens 74-77 18022597-2 2007 We previously demonstrated that the effect of sodium bicarbonate on pHi depends on the non-bicarbonate buffering system. Sodium Bicarbonate 46-64 glucose-6-phosphate isomerase Homo sapiens 68-71 18022597-2 2007 We previously demonstrated that the effect of sodium bicarbonate on pHi depends on the non-bicarbonate buffering system. Bicarbonates 53-64 glucose-6-phosphate isomerase Homo sapiens 68-71 18022597-6 2007 The pHi was measured with the pH-sensitive fluorescent dye bis-carboxyethyl carboxy-fluorescein (BCECF). bis-carboxyethyl carboxy-fluorescein 59-95 glucose-6-phosphate isomerase Homo sapiens 4-7 17826995-2 2007 3-[H-Dmt-NH-(CH(2))(4)]-6-beta-phenethyl-5-methyl-2(1H)-pyrazinone 11 bound to mu-opioid receptors with high affinity (K(i)mu=0.13 nM; K(i)delta/K(i)mu=447) with mu-agonism (GPI IC(50)=15.9 nM) and weak delta-antagonism (MVD pA(2)=6.35). -[h-dmt-nh-( 1-13 glucose-6-phosphate isomerase Homo sapiens 174-177 17826995-2 2007 3-[H-Dmt-NH-(CH(2))(4)]-6-beta-phenethyl-5-methyl-2(1H)-pyrazinone 11 bound to mu-opioid receptors with high affinity (K(i)mu=0.13 nM; K(i)delta/K(i)mu=447) with mu-agonism (GPI IC(50)=15.9 nM) and weak delta-antagonism (MVD pA(2)=6.35). phenethyl-5-methyl-2(1h)-pyrazinone 31-66 glucose-6-phosphate isomerase Homo sapiens 174-177 17877362-2 2007 After a trimannose and a phospholipidated pseudodisaccharide were prepared separately, they were coupled together to form the GPI core, which was then phosphorylated to introduce two phosphoethanolamine moieties in one step to afford CD52 GPI in its fully protected form. trimannose 8-18 glucose-6-phosphate isomerase Homo sapiens 126-129 17877362-2 2007 After a trimannose and a phospholipidated pseudodisaccharide were prepared separately, they were coupled together to form the GPI core, which was then phosphorylated to introduce two phosphoethanolamine moieties in one step to afford CD52 GPI in its fully protected form. pseudodisaccharide 42-60 glucose-6-phosphate isomerase Homo sapiens 126-129 17877362-2 2007 After a trimannose and a phospholipidated pseudodisaccharide were prepared separately, they were coupled together to form the GPI core, which was then phosphorylated to introduce two phosphoethanolamine moieties in one step to afford CD52 GPI in its fully protected form. phosphorylethanolamine 183-202 glucose-6-phosphate isomerase Homo sapiens 126-129 17604179-9 2007 The quantum yield of oxygen uptake Phi(-O2) as a measure of formation of hydrogen peroxide increases with increasing amino acid concentration, approaching Phi(-O2) for AQ in air-saturated solution. Oxygen 21-27 glucose-6-phosphate isomerase Homo sapiens 155-158 17604179-9 2007 The quantum yield of oxygen uptake Phi(-O2) as a measure of formation of hydrogen peroxide increases with increasing amino acid concentration, approaching Phi(-O2) for AQ in air-saturated solution. Hydrogen Peroxide 73-90 glucose-6-phosphate isomerase Homo sapiens 35-38 17604179-9 2007 The quantum yield of oxygen uptake Phi(-O2) as a measure of formation of hydrogen peroxide increases with increasing amino acid concentration, approaching Phi(-O2) for AQ in air-saturated solution. Hydrogen Peroxide 73-90 glucose-6-phosphate isomerase Homo sapiens 155-158 17604179-9 2007 The quantum yield of oxygen uptake Phi(-O2) as a measure of formation of hydrogen peroxide increases with increasing amino acid concentration, approaching Phi(-O2) for AQ in air-saturated solution. 9,10-anthraquinone 168-170 glucose-6-phosphate isomerase Homo sapiens 35-38 17604179-9 2007 The quantum yield of oxygen uptake Phi(-O2) as a measure of formation of hydrogen peroxide increases with increasing amino acid concentration, approaching Phi(-O2) for AQ in air-saturated solution. 9,10-anthraquinone 168-170 glucose-6-phosphate isomerase Homo sapiens 155-158 17714445-0 2007 CWH43 is required for the introduction of ceramides into GPI anchors in Saccharomyces cerevisiae. Ceramides 42-51 glucose-6-phosphate isomerase Homo sapiens 57-60 19662214-2 2007 Since pH is the influential factor in the Bohr-Haldane effect, pHi is actively maintained via secondary active transports Na(+)/H(+) exchange and HC(3) (-)/Cl(-) anion exchanger. bohr 42-46 glucose-6-phosphate isomerase Homo sapiens 63-66 19662214-2 2007 Since pH is the influential factor in the Bohr-Haldane effect, pHi is actively maintained via secondary active transports Na(+)/H(+) exchange and HC(3) (-)/Cl(-) anion exchanger. haldane 47-54 glucose-6-phosphate isomerase Homo sapiens 63-66 17714445-1 2007 After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, the fatty acid in sn-2 of the diacylglycerol moiety can be replaced by a C26:0 fatty acid by a deacylation-reacylation cycle catalysed by Per1p and Gup1p. Glycosylphosphatidylinositols 6-35 glucose-6-phosphate isomerase Homo sapiens 37-40 17714445-1 2007 After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, the fatty acid in sn-2 of the diacylglycerol moiety can be replaced by a C26:0 fatty acid by a deacylation-reacylation cycle catalysed by Per1p and Gup1p. Fatty Acids 102-112 glucose-6-phosphate isomerase Homo sapiens 37-40 17714445-1 2007 After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, the fatty acid in sn-2 of the diacylglycerol moiety can be replaced by a C26:0 fatty acid by a deacylation-reacylation cycle catalysed by Per1p and Gup1p. SN 2 116-120 glucose-6-phosphate isomerase Homo sapiens 37-40 17714445-1 2007 After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, the fatty acid in sn-2 of the diacylglycerol moiety can be replaced by a C26:0 fatty acid by a deacylation-reacylation cycle catalysed by Per1p and Gup1p. Diglycerides 128-142 glucose-6-phosphate isomerase Homo sapiens 37-40 17714445-1 2007 After glycosylphosphatidylinositols (GPIs) are added to GPI proteins of Saccharomyces cerevisiae, the fatty acid in sn-2 of the diacylglycerol moiety can be replaced by a C26:0 fatty acid by a deacylation-reacylation cycle catalysed by Per1p and Gup1p. c26:0 fatty acid 171-187 glucose-6-phosphate isomerase Homo sapiens 37-40 17504845-7 2007 CONCLUSION: HD patients having low PHCO3 exhibited low neutrophil pHi. phco3 35-40 glucose-6-phosphate isomerase Homo sapiens 66-69 17714445-2 2007 Furthermore the diacylglycerol moiety of the yeast GPI anchor can also be replaced by ceramides. Diglycerides 16-30 glucose-6-phosphate isomerase Homo sapiens 51-54 17714445-2 2007 Furthermore the diacylglycerol moiety of the yeast GPI anchor can also be replaced by ceramides. Ceramides 86-95 glucose-6-phosphate isomerase Homo sapiens 51-54 17714445-3 2007 CWH43 of yeast is homologous to PGAP2, a gene that recently was implicated in a similar deacylation reacylation cycle of GPI proteins in mammalian cells, where PGAP2 is required for the reacylation of monoradylglycerol-type GPI anchors. monoradylglycerol 201-218 glucose-6-phosphate isomerase Homo sapiens 121-124 17714445-3 2007 CWH43 of yeast is homologous to PGAP2, a gene that recently was implicated in a similar deacylation reacylation cycle of GPI proteins in mammalian cells, where PGAP2 is required for the reacylation of monoradylglycerol-type GPI anchors. monoradylglycerol 201-218 glucose-6-phosphate isomerase Homo sapiens 224-227 17611644-8 2007 In addition, the IgH-CDR3 of anti-GPI Ab-positive patients was rich in basic-ionized amino acids (arginine, histidine, and lysine) near their central position, suggesting a high affinity. Arginine 98-106 glucose-6-phosphate isomerase Homo sapiens 34-37 17718606-0 2007 Quadrupole, octopole, and hexadecapole electric moments of Sigma, Pi, Delta, and Phi electronic states: cylindrically asymmetric charge density distributions in linear molecules with nonzero electronic angular momentum. quadrupole 0-10 glucose-6-phosphate isomerase Homo sapiens 81-84 17718606-0 2007 Quadrupole, octopole, and hexadecapole electric moments of Sigma, Pi, Delta, and Phi electronic states: cylindrically asymmetric charge density distributions in linear molecules with nonzero electronic angular momentum. hexadecapole 26-38 glucose-6-phosphate isomerase Homo sapiens 81-84 17631555-7 2007 This intermediate was then conjugated to adamantane-trimerized GPI (2[(3-amino-3-carboxypropyl)(hydroxy)(phosphinyl)-methyl]pentane-1,5-dioic acid) in 1 step with >95% yield and no need for HPLC purification. Adamantane 41-51 glucose-6-phosphate isomerase Homo sapiens 63-66 17631555-7 2007 This intermediate was then conjugated to adamantane-trimerized GPI (2[(3-amino-3-carboxypropyl)(hydroxy)(phosphinyl)-methyl]pentane-1,5-dioic acid) in 1 step with >95% yield and no need for HPLC purification. 2[(3-amino-3-carboxypropyl)(hydroxy)(phosphinyl)-methyl]pentane-1,5-dioic acid 68-146 glucose-6-phosphate isomerase Homo sapiens 63-66 17611644-8 2007 In addition, the IgH-CDR3 of anti-GPI Ab-positive patients was rich in basic-ionized amino acids (arginine, histidine, and lysine) near their central position, suggesting a high affinity. Histidine 108-117 glucose-6-phosphate isomerase Homo sapiens 34-37 17611644-8 2007 In addition, the IgH-CDR3 of anti-GPI Ab-positive patients was rich in basic-ionized amino acids (arginine, histidine, and lysine) near their central position, suggesting a high affinity. Lysine 123-129 glucose-6-phosphate isomerase Homo sapiens 34-37 17188004-2 2007 In the present study, we investigated the pH-dependence of phorbol myristate acetate (PMA)-induced PMN spreading. Tetradecanoylphorbol Acetate 59-84 glucose-6-phosphate isomerase Homo sapiens 42-44 17512540-6 2007 The parameter Phi(H/D), defined as the ratio of the effect of isotopic substitution upon the activation free energy to the equilibrium free energy was determined to be 0.6 in a D(2)O background and 0.75 in a H(2)O background, indicating that significant intraprotein hydrogen bond interactions are developed in the transition state for the folding of NTL9. Hydrogen 267-275 glucose-6-phosphate isomerase Homo sapiens 14-17 17483335-1 2007 Phosphoglucose isomerase (PGI) is one of the glycolytic enzymes and is a multifunctional enzyme that functions in glucose metabolism inside the cell while acting as a cytokine outside the cell, with properties that include autocrine motility factor (AMF) regulating tumor cell motility. Glucose 7-14 glucose-6-phosphate isomerase Homo sapiens 26-29 17483335-1 2007 Phosphoglucose isomerase (PGI) is one of the glycolytic enzymes and is a multifunctional enzyme that functions in glucose metabolism inside the cell while acting as a cytokine outside the cell, with properties that include autocrine motility factor (AMF) regulating tumor cell motility. Glucose 7-14 glucose-6-phosphate isomerase Homo sapiens 223-248 17483335-1 2007 Phosphoglucose isomerase (PGI) is one of the glycolytic enzymes and is a multifunctional enzyme that functions in glucose metabolism inside the cell while acting as a cytokine outside the cell, with properties that include autocrine motility factor (AMF) regulating tumor cell motility. Glucose 7-14 glucose-6-phosphate isomerase Homo sapiens 250-253 17536611-1 2007 Primary hyperoxaluria type I (PH I) is a rare recessive autosomal disorder characterized by systemic calcium oxalate depositions, that results in renal failure and systemic oxalosis. Calcium Oxalate 101-116 glucose-6-phosphate isomerase Homo sapiens 0-28 17536611-1 2007 Primary hyperoxaluria type I (PH I) is a rare recessive autosomal disorder characterized by systemic calcium oxalate depositions, that results in renal failure and systemic oxalosis. Calcium Oxalate 101-116 glucose-6-phosphate isomerase Homo sapiens 30-34 17209136-5 2007 Since HPV requires Ca2+ influx into PASMCs and can be modulated by pH, we hypothesized that AZ alters hypoxia-induced changes in PASMC intracellular pH (pH(i)) or Ca2+ concentration ([Ca2+](i)). Acetazolamide 92-94 glucose-6-phosphate isomerase Homo sapiens 153-158 17241995-3 2007 Chlorophyll a fluorescence analysis revealed differences in quantum yield efficiency of photosystem II of light adapted leaves (Phi(PSII)) among shoot types and in response to girdling. chlorophyll a 0-13 glucose-6-phosphate isomerase Homo sapiens 128-137 17188004-2 2007 In the present study, we investigated the pH-dependence of phorbol myristate acetate (PMA)-induced PMN spreading. Tetradecanoylphorbol Acetate 86-89 glucose-6-phosphate isomerase Homo sapiens 42-44 17200954-8 2007 RESULTS: The pHi clamping conditions were optimized as 140 mM potassium and 10 microM nigericin. Potassium 62-71 glucose-6-phosphate isomerase Homo sapiens 13-16 17013814-10 2007 The reduced burst effectiveness of GPI-defective granulocytes was maintained after treatment with phorbol 12-myristate 13-acetate, a pharmacological stimulus able to extensively recruit and to trigger intracellular protein kinase C (PKC). Tetradecanoylphorbol Acetate 98-129 glucose-6-phosphate isomerase Homo sapiens 35-38 17200954-8 2007 RESULTS: The pHi clamping conditions were optimized as 140 mM potassium and 10 microM nigericin. Nigericin 86-95 glucose-6-phosphate isomerase Homo sapiens 13-16 16983661-5 2006 Furthermore, inhibition of ERK activation with the UO126 compound totally prevented OPC differentiation in response to pH(i) shift. U 0126 51-56 glucose-6-phosphate isomerase Homo sapiens 119-121 17308069-9 2007 Although both hexokinase and phosphoglucose isomerase are known to interact with 2-DG, our findings of increased levels of hexokinase more likely implicate this enzyme in the mechanism of HIF-mediated resistance to 2-DG. Deoxyglucose 81-85 glucose-6-phosphate isomerase Homo sapiens 29-53 17068143-5 2007 The CSF-1-induced rise in pH(i) is not blocked by 4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid, an inhibitor of HCO(3)(-) transporters but is abolished by removing extracellular sodium. Sodium 184-190 glucose-6-phosphate isomerase Homo sapiens 26-31 16983661-7 2006 We also show that this pH(i) pathway is involved in the process of retinoic acid-induced OPC differentiation. Tretinoin 67-80 glucose-6-phosphate isomerase Homo sapiens 23-25 16971185-8 2006 On the PGI, all patients rated themselves as better and as having less disfluent speech after LEV therapy. Levetiracetam 94-97 glucose-6-phosphate isomerase Homo sapiens 7-10 16949552-7 2006 At pHi 7.2, currents in response to voltage steps positive to EK were essentially time independent for Kir4.2 indicating rapid block by Mg2+. magnesium ion 136-140 glucose-6-phosphate isomerase Homo sapiens 3-6 16971185-10 2006 The beneficial effect of LEV on verbal disfluency demonstrated on the PGI persisted for the entire period of observation, which ranged from 7 to 11 months. Levetiracetam 25-28 glucose-6-phosphate isomerase Homo sapiens 70-73 17147109-1 2006 OBJECTIVE: To investigate the function and mechanism of 3-(5"-hydroxymethyl-2"-furyl)-1-benzylindazole (YC-1) on activity of VEGF and GPI genes in human pancreatic cancer PC-3 cells incubated under hypoxic conditions. 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole 56-102 glucose-6-phosphate isomerase Homo sapiens 134-137 17077902-4 2006 The quantum yield of oxygen uptake (Phi(-O2)) increases with increasing donor concentration. Oxygen 21-27 glucose-6-phosphate isomerase Homo sapiens 36-39 17077902-5 2006 Phi(-O2) = 0.3-0.6 in the presence of 1 M 2-propanol and 3-10 mM ascorbic acid or EDTA. 2-Propanol 42-52 glucose-6-phosphate isomerase Homo sapiens 0-3 17077902-5 2006 Phi(-O2) = 0.3-0.6 in the presence of 1 M 2-propanol and 3-10 mM ascorbic acid or EDTA. Ascorbic Acid 65-78 glucose-6-phosphate isomerase Homo sapiens 0-3 17077902-5 2006 Phi(-O2) = 0.3-0.6 in the presence of 1 M 2-propanol and 3-10 mM ascorbic acid or EDTA. Edetic Acid 82-86 glucose-6-phosphate isomerase Homo sapiens 0-3 17077902-6 2006 The properties of the quinone and donor radicals involved and the pH and concentration dependences of Phi(-O2) are described. quinone 22-29 glucose-6-phosphate isomerase Homo sapiens 102-105 16965582-7 2006 RESULTS: Storage in 175 mL of the chloride-free, hypotonic medium at a hematocrit (Hct) level of 59 to 60 percent resulted in an elevated pHi and the maintenance of 2,3-DPG at or above the initial value for 2 weeks without loss of ATP. Chlorides 34-42 glucose-6-phosphate isomerase Homo sapiens 138-141 16965582-10 2006 CONCLUSION: Improvements in the maintenance of 2,3-DPG were achieved with 175 mL of a chloride-free storage solution with familiar additives at nontoxic concentrations to increase pHi. 2,3-Diphosphoglycerate 47-54 glucose-6-phosphate isomerase Homo sapiens 180-183 16808493-6 2006 Reaction optimization studies suggest that PhI(O2CCF3)2/BF3.OEt2 or MoCl5 are superior to FeCl3 or AlCl3/CuCl2. boron trifluoride 56-59 glucose-6-phosphate isomerase Homo sapiens 43-53 16808493-6 2006 Reaction optimization studies suggest that PhI(O2CCF3)2/BF3.OEt2 or MoCl5 are superior to FeCl3 or AlCl3/CuCl2. Ether 60-64 glucose-6-phosphate isomerase Homo sapiens 43-53 16808493-6 2006 Reaction optimization studies suggest that PhI(O2CCF3)2/BF3.OEt2 or MoCl5 are superior to FeCl3 or AlCl3/CuCl2. molybdenum chloride 68-73 glucose-6-phosphate isomerase Homo sapiens 43-53 16814773-7 2006 Lesions restricted to posterior motor portions of GPi produced selective degeneration of LF and only damaged AL if the lesions extended more anteriorly. Aluminum 109-111 glucose-6-phosphate isomerase Homo sapiens 50-53 16931986-7 2006 By contrast, the open probability of truncated Kir6.2DeltaC26, which forms a functional channel without SUR2A, was attenuated by isoflurane at both pHi 7.4 and pHi 6.8. Isoflurane 129-139 glucose-6-phosphate isomerase Homo sapiens 148-151 16931986-7 2006 By contrast, the open probability of truncated Kir6.2DeltaC26, which forms a functional channel without SUR2A, was attenuated by isoflurane at both pHi 7.4 and pHi 6.8. Isoflurane 129-139 glucose-6-phosphate isomerase Homo sapiens 160-163 16931986-8 2006 Coexpression of Kir6.2DeltaC26 with SUR2A restored pHi sensitivity of channel activation by isoflurane. Isoflurane 92-102 glucose-6-phosphate isomerase Homo sapiens 51-54 16931986-9 2006 Site-directed mutagenesis within the Walker motifs of SUR2A abolished isoflurane activation of KATP channel at pHi 6.8. Isoflurane 70-80 glucose-6-phosphate isomerase Homo sapiens 111-114 16931986-11 2006 CONCLUSIONS: The nucleotide binding domains of SUR2A play a crucial role in isoflurane facilitation of the KATP channel activity at moderately acidic pHi as would occur during early ischemia. Isoflurane 76-86 glucose-6-phosphate isomerase Homo sapiens 150-153 16963074-3 2006 In this study, enhanced (EGFP) was found to be a very sensitive indicator of pHi in in vitro cell culture, and responded rapidly to extracellular pH (pHe) changes. Phenylalanine 150-153 glucose-6-phosphate isomerase Homo sapiens 77-80 16963074-5 2006 EGFP intensity was found to reflect pHi values of cells at different pHe in the presence of nigericin and was affected by the addition of HCl and NaOH. Phenylalanine 69-72 glucose-6-phosphate isomerase Homo sapiens 36-39 16963074-5 2006 EGFP intensity was found to reflect pHi values of cells at different pHe in the presence of nigericin and was affected by the addition of HCl and NaOH. Nigericin 92-101 glucose-6-phosphate isomerase Homo sapiens 36-39 16963074-5 2006 EGFP intensity was found to reflect pHi values of cells at different pHe in the presence of nigericin and was affected by the addition of HCl and NaOH. Hydrochloric Acid 138-141 glucose-6-phosphate isomerase Homo sapiens 36-39 16963074-5 2006 EGFP intensity was found to reflect pHi values of cells at different pHe in the presence of nigericin and was affected by the addition of HCl and NaOH. Sodium Hydroxide 146-150 glucose-6-phosphate isomerase Homo sapiens 36-39 16740258-7 2006 Inhibition of glucose metabolism or V-ATPase impaired neutrophil spreading, blocked the increase in the pHi and induced extrusion of membrane tubulovesicular extensions (cytonemes), anchoring cells to substrata. Glucose 14-21 glucose-6-phosphate isomerase Homo sapiens 104-107 16563432-0 2006 The autocrine motility factor (AMF) and AMF-receptor combination needs sugar chain recognition ability and interaction using the C-terminal region of AMF. Sugars 71-76 glucose-6-phosphate isomerase Homo sapiens 4-29 16775143-6 2006 Under STN blockade, the duration of single Put stimulation induced gabazine (a GABA(A) antagonist)-sensitive responses differed greatly in the GPe ( approximately 400 ms long) and in the GPi (60 ms long). gabazine 67-75 glucose-6-phosphate isomerase Homo sapiens 187-190 16775143-7 2006 Burst stimulation of the Put induced CGP55845 [(2S)-3-[[(1S)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl)phosphinic acid] (a GABA(B) antagonist)-sensitive responses in the GPe and GPi. (2s)-3-[[(1s)-1-(3,4-dichlorophenyl)ethyl]amino-2-hydroxypropyl](phenylmethyl)phosphinic acid 47-140 glucose-6-phosphate isomerase Homo sapiens 200-203 16775143-9 2006 Local CGP55845 application increased the spontaneous firing of GPe and GPi neurons, suggesting that GABA released from the axons of GPe neurons effectively activates GABA(B) receptors in the GPe and GPi and contributes significantly to the control of the level of neuronal activity. gamma-Aminobutyric Acid 100-104 glucose-6-phosphate isomerase Homo sapiens 71-74 16775143-9 2006 Local CGP55845 application increased the spontaneous firing of GPe and GPi neurons, suggesting that GABA released from the axons of GPe neurons effectively activates GABA(B) receptors in the GPe and GPi and contributes significantly to the control of the level of neuronal activity. gamma-Aminobutyric Acid 100-104 glucose-6-phosphate isomerase Homo sapiens 199-202 16734821-2 2006 Whether trehalose-loaded freeze-dried and rehydrated PLTs could regulate intracellular pH (pHi) was evaluated. Trehalose 8-17 glucose-6-phosphate isomerase Homo sapiens 91-94 16734821-5 2006 pHi was measured in resting cells, cells acidified with nigericin, and cells treated with thrombin. Nigericin 56-65 glucose-6-phosphate isomerase Homo sapiens 0-3 16734821-8 2006 Nigericin treatment of cells showed that the recovery in pHi was Na+-dependent and followed Michaelis-Menten kinetics. Nigericin 0-9 glucose-6-phosphate isomerase Homo sapiens 57-60 16563432-0 2006 The autocrine motility factor (AMF) and AMF-receptor combination needs sugar chain recognition ability and interaction using the C-terminal region of AMF. Sugars 71-76 glucose-6-phosphate isomerase Homo sapiens 31-34 16563432-0 2006 The autocrine motility factor (AMF) and AMF-receptor combination needs sugar chain recognition ability and interaction using the C-terminal region of AMF. Sugars 71-76 glucose-6-phosphate isomerase Homo sapiens 40-43 16563432-0 2006 The autocrine motility factor (AMF) and AMF-receptor combination needs sugar chain recognition ability and interaction using the C-terminal region of AMF. Sugars 71-76 glucose-6-phosphate isomerase Homo sapiens 40-43 16563432-7 2006 Mutation of residues that interact with the phosphate group of the PHI substrate significantly reduced the cell motility-stimulating activity. Phosphates 44-53 glucose-6-phosphate isomerase Homo sapiens 67-70 16563432-10 2006 Mutant AMFR lacking the N-sugar chain was expressed on the cell membrane but did not respond to AMF-stimulation, and N-glycosidase-treated AMFR did not compete with receptor binding of AMF. n-sugar 24-31 glucose-6-phosphate isomerase Homo sapiens 7-10 16563432-12 2006 These results suggest that the N-glyco side-chain of AMFR is a trigger and that interaction between the 117-C-terminal part of AMF and the extracellular core protein of AMFR is needed during AMF-AMFR interactions. Nitrogen 31-32 glucose-6-phosphate isomerase Homo sapiens 53-56 16563432-12 2006 These results suggest that the N-glyco side-chain of AMFR is a trigger and that interaction between the 117-C-terminal part of AMF and the extracellular core protein of AMFR is needed during AMF-AMFR interactions. Nitrogen 31-32 glucose-6-phosphate isomerase Homo sapiens 127-130 16785205-7 2006 GPI8 functions dually to perform the proteolytic cleavage of the C-terminal signal sequence of the precursor protein, followed by the formation of an amide bond between the protein and the ethanolamine phosphate of the GPI. Amides 150-155 glucose-6-phosphate isomerase Homo sapiens 0-3 16785205-7 2006 GPI8 functions dually to perform the proteolytic cleavage of the C-terminal signal sequence of the precursor protein, followed by the formation of an amide bond between the protein and the ethanolamine phosphate of the GPI. phosphorylethanolamine 189-211 glucose-6-phosphate isomerase Homo sapiens 0-3 16785205-9 2006 We describe recently published studies that have identified other potential components of the TAM complex and that have elucidated their likely role in protein-GPI attachment. tam 94-97 glucose-6-phosphate isomerase Homo sapiens 160-163 16601836-10 2006 Aspirin treatment reduced PGI-M already at the lowest dosage (by approximately 25%), but PGI-M excretion and platelet aggregability were not correlated. Aspirin 0-7 glucose-6-phosphate isomerase Homo sapiens 26-29 16506866-3 2006 The quantum yield of oxygen uptake (Phi(-O(2))) increases with increasing 2-propanol concentration up to 0.9. Oxygen 21-27 glucose-6-phosphate isomerase Homo sapiens 36-39 16506866-3 2006 The quantum yield of oxygen uptake (Phi(-O(2))) increases with increasing 2-propanol concentration up to 0.9. 2-Propanol 74-84 glucose-6-phosphate isomerase Homo sapiens 36-39 16506866-6 2006 For the ketones in the presence of 3-30 mM ascorbic acid or triethylamine Phi(-O(2)) = 0.3-0.9. Ketones 8-15 glucose-6-phosphate isomerase Homo sapiens 74-77 16506866-6 2006 For the ketones in the presence of 3-30 mM ascorbic acid or triethylamine Phi(-O(2)) = 0.3-0.9. triethylamine 60-73 glucose-6-phosphate isomerase Homo sapiens 74-77 16506866-7 2006 The specific properties of ketones, including 4-methoxyacetophenone and 2-acetonaphthone, the radicals involved and the pH and concentration dependences of Phi(-O(2)) are discussed. Ketones 27-34 glucose-6-phosphate isomerase Homo sapiens 156-159 16564041-7 2006 Intracellular pH (pHi) was measured using the pH-sensitive fluorescent probe bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF)-AM. bis-(2-carboxyethyl)-5(6)-carboxyfluorescein 77-121 glucose-6-phosphate isomerase Homo sapiens 18-21 16564041-7 2006 Intracellular pH (pHi) was measured using the pH-sensitive fluorescent probe bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF)-AM. bcecf 123-128 glucose-6-phosphate isomerase Homo sapiens 18-21 16564041-8 2006 NHE activity was determined as Na+-induced pHi recovery from an acid load achieved by luminal exposure to 40 mmol/l NH4Cl. Ammonium Chloride 116-121 glucose-6-phosphate isomerase Homo sapiens 43-46 16564041-12 2006 Dexamethasone increased the Na+-induced pHi recovery rate which was reversed by the inhibition of NHE3 with 650 microM of HOE694. Dexamethasone 0-13 glucose-6-phosphate isomerase Homo sapiens 40-43 16930575-3 2006 However, the relationship between pHi and ROS generation is not well understood. Reactive Oxygen Species 42-45 glucose-6-phosphate isomerase Homo sapiens 34-37 16599643-4 2006 Competition experiments indicated the following relative reactivities of dehalogenation by SmI2: PhI/PhCl-Cr(CO)3/PhBr/PhCl = 50:1:0.3:<0.001. samarium diiodide 91-95 glucose-6-phosphate isomerase Homo sapiens 97-100 16481580-6 2006 Blockade of the Na+/H+ exchanger (NHE) with cariporide (5 micromol l-1) produced a complete inhibition of pHi restitution, without affecting the mechanical recovery. cariporide 44-54 glucose-6-phosphate isomerase Homo sapiens 106-109 16261161-0 2006 GPI-anchored TIMP-1 treatment renders renal cell carcinoma sensitive to FAS-meditated killing. ammonium ferrous sulfate 72-75 glucose-6-phosphate isomerase Homo sapiens 0-3 16261161-4 2006 A glycosylphosphatidylinositol (GPI) anchor was fused to TIMP-1 to focus defined concentrations of this inhibitory protein on the surface of three renal cell carcinoma (RCC) cell lines (RCC-26, RCC-53 and A498) independently of cell surface protein-protein interactions. Glycosylphosphatidylinositols 2-30 glucose-6-phosphate isomerase Homo sapiens 32-35 16288820-6 2006 When Tween 40 was added to gD and GPI-0100, mean peak lesion scores were also significantly reduced. polysorbate 40 5-13 glucose-6-phosphate isomerase Homo sapiens 34-37 16501226-5 2006 We find that, unless the change in free energy associated with the probing mutation is quite large, the precision of phi-values is relatively poor when determined using rates extrapolated to the absence of denaturant. denaturant 206-216 glucose-6-phosphate isomerase Homo sapiens 117-120 16365698-1 2006 The synthesis, photophysical characterization, and determination of singlet oxygen quantum yields (Phi(Delta)) for a class of fluorene derivatives with potential application in two-photon photodynamic therapy (PDT) is reported. Singlet Oxygen 68-82 glucose-6-phosphate isomerase Homo sapiens 99-109 16497028-5 2006 The effective interaction potential between a pair of ethanol beads, Phi(R), is approximated at three levels of sophistication. Ethanol 54-61 glucose-6-phosphate isomerase Homo sapiens 69-72 16497028-6 2006 At the lowest one, we approximate Phi(R) by the potential of mean force between the centers of mass of two ethanol beads calculated in the fully atomistic MD simulations; at the second level, we take Phi(R) to be the potential linked to total and direct correlation functions in the hypernetted-chain closure of the Ornstein-Zernike equation. Ethanol 107-114 glucose-6-phosphate isomerase Homo sapiens 34-37 16475697-11 2006 DISCUSSION: Based on results of the in vivo and in vitro studies, it is suggested that malignant musculoskeletal tumors have a large deltapH between the pHi and the pHe or between the pHi and the vaculolar pH and also that a large ApH increases AO accumulation in tumors. Phenylalanine 165-168 glucose-6-phosphate isomerase Homo sapiens 153-156 16475697-11 2006 DISCUSSION: Based on results of the in vivo and in vitro studies, it is suggested that malignant musculoskeletal tumors have a large deltapH between the pHi and the pHe or between the pHi and the vaculolar pH and also that a large ApH increases AO accumulation in tumors. Acridine Orange 245-247 glucose-6-phosphate isomerase Homo sapiens 153-156 16475697-13 2006 CONCLUSION: AO accumulation in musculoskeletal tumors was dependent on the ApH between the pHi and the pHe, or between the pHi and the vacuolar pH. Acridine Orange 12-14 glucose-6-phosphate isomerase Homo sapiens 91-94 16475697-13 2006 CONCLUSION: AO accumulation in musculoskeletal tumors was dependent on the ApH between the pHi and the pHe, or between the pHi and the vacuolar pH. Acridine Orange 12-14 glucose-6-phosphate isomerase Homo sapiens 123-126 16604431-1 2006 The quantum yield, Phi(Delta), of singlet oxygen generation under two-photon excitation has been determined for a fluorene derivative. Singlet Oxygen 34-48 glucose-6-phosphate isomerase Homo sapiens 19-22 16604431-1 2006 The quantum yield, Phi(Delta), of singlet oxygen generation under two-photon excitation has been determined for a fluorene derivative. fluorene 114-122 glucose-6-phosphate isomerase Homo sapiens 19-22 16604431-4 2006 Fluorene 1 exhibited relatively high singlet oxygen quantum yield, Phi(Delta) approximately 0.4 +/- 0.1, and had a two-photon absorption cross-section of 28 +/- 5 GM. fluorene 0-8 glucose-6-phosphate isomerase Homo sapiens 67-70 16365698-3 2006 A photochemical method, using 1,3-diphenylisobenzofuran (DPBF) as 1O2 chemical quencher, was employed to determine the singlet oxygen quantum yields (Phi(Delta)) of the fluorene-based photosensitizers in ethanol. fluorene 169-177 glucose-6-phosphate isomerase Homo sapiens 150-160 16365698-3 2006 A photochemical method, using 1,3-diphenylisobenzofuran (DPBF) as 1O2 chemical quencher, was employed to determine the singlet oxygen quantum yields (Phi(Delta)) of the fluorene-based photosensitizers in ethanol. Ethanol 204-211 glucose-6-phosphate isomerase Homo sapiens 150-160 16365698-1 2006 The synthesis, photophysical characterization, and determination of singlet oxygen quantum yields (Phi(Delta)) for a class of fluorene derivatives with potential application in two-photon photodynamic therapy (PDT) is reported. fluorene 126-134 glucose-6-phosphate isomerase Homo sapiens 99-109 16365698-3 2006 A photochemical method, using 1,3-diphenylisobenzofuran (DPBF) as 1O2 chemical quencher, was employed to determine the singlet oxygen quantum yields (Phi(Delta)) of the fluorene-based photosensitizers in ethanol. 1,3-diphenylisobenzofuran 30-55 glucose-6-phosphate isomerase Homo sapiens 150-160 16365698-3 2006 A photochemical method, using 1,3-diphenylisobenzofuran (DPBF) as 1O2 chemical quencher, was employed to determine the singlet oxygen quantum yields (Phi(Delta)) of the fluorene-based photosensitizers in ethanol. 1,3-diphenylisobenzofuran 57-61 glucose-6-phosphate isomerase Homo sapiens 150-160 16365698-3 2006 A photochemical method, using 1,3-diphenylisobenzofuran (DPBF) as 1O2 chemical quencher, was employed to determine the singlet oxygen quantum yields (Phi(Delta)) of the fluorene-based photosensitizers in ethanol. Singlet Oxygen 119-133 glucose-6-phosphate isomerase Homo sapiens 150-160 16449818-3 2006 The modulation of vasotone by pHi depends on the type of blood vessel as well as on the pattern of regulatory input signals. vasotone 18-26 glucose-6-phosphate isomerase Homo sapiens 30-33 16380753-1 2005 OBJECTIVE: This study aimed to evaluate the sensitivity and specificity of the anti-&beta;2-glycoprotein I (GPI) antibodies for pregnancy morbidity in the antiphosoplipid syndrome (APS). Adenosine Monophosphate 85-88 glucose-6-phosphate isomerase Homo sapiens 112-115 16335927-3 2005 Regardless of the type of linker between the Dmt-Tic pharmacophores, delta-opioid-mediated antagonism was extraordinarily high in all analogues (pA2 = 10.42-11.28), while in vitro agonism (MVD and GPI bioassays) was essentially absent (ca. delta-opioid 69-81 glucose-6-phosphate isomerase Homo sapiens 197-200 16291721-10 2005 Release is likely due to the exclusive use of C-14:0 myristate in the bloodstream stage GPI anchor. Carbon 46-47 glucose-6-phosphate isomerase Homo sapiens 88-91 15972387-3 2005 It was found that intracellular O(2)-* levels in these cells were increased in parallel to the elevation of pH(i) by outflow of H(+) induced via NH(4)Cl loading followed by rapid removal. Superoxides 32-36 glucose-6-phosphate isomerase Homo sapiens 108-113 16248668-3 2005 The polymers with m = 12, 14, and 16 exhibit also a hexagonal columnar crystal phase (Phi(h,k)). Polymers 4-12 glucose-6-phosphate isomerase Homo sapiens 86-89 15972387-3 2005 It was found that intracellular O(2)-* levels in these cells were increased in parallel to the elevation of pH(i) by outflow of H(+) induced via NH(4)Cl loading followed by rapid removal. Ammonium Chloride 145-152 glucose-6-phosphate isomerase Homo sapiens 108-113 15856280-4 2005 Under experimental conditions that reduce the intrinsic Na/H exchanger activity, exposure of control cells to a 10 mM NH4Cl- containing solution induces the classic pHi response profile of cells having a high permeability to NH3 (PNH3) but relatively low permeability to NH4+ (PNH4). Ammonium Chloride 118-123 glucose-6-phosphate isomerase Homo sapiens 165-168 16040608-2 2005 By using an intracellular pH (pH(i)) spectrofluorescence imaging system and the NH4Cl acid-loading technique, we have shown that the synthetic glucocorticoid,dexamethasone, accelerated intracellular pH recovery after an acid load in a human bronchial epithelial cell line (16HBE14o- cells). Dexamethasone 158-171 glucose-6-phosphate isomerase Homo sapiens 30-35 16040608-3 2005 Exposure to NH4Cl (20 mm) elicited an intracellular acidification, followed by a pH(i) recovery. Ammonium Chloride 12-17 glucose-6-phosphate isomerase Homo sapiens 81-86 16040608-4 2005 Inhibition of the Na+/H+ exchanger decreased the steady-state pH(i) and antagonized the dexamethasone stimulation of pH(i) regulation. Dexamethasone 88-101 glucose-6-phosphate isomerase Homo sapiens 117-122 16040608-5 2005 The rapid effect of dexamethasone on pH(i) was neither affected by the inhibitor of transcription, cycloheximide, nor by the classical glucocorticoid and mineralocorticoid receptors antagonists, RU486 and spironolactone, respectively. Dexamethasone 20-33 glucose-6-phosphate isomerase Homo sapiens 37-42 16040608-5 2005 The rapid effect of dexamethasone on pH(i) was neither affected by the inhibitor of transcription, cycloheximide, nor by the classical glucocorticoid and mineralocorticoid receptors antagonists, RU486 and spironolactone, respectively. Spironolactone 205-219 glucose-6-phosphate isomerase Homo sapiens 37-42 16040608-6 2005 The dexamethasone effect on pH(i) regulation was reduced by inhibitors of adenylate cyclase, cAMP-dependent protein kinase and mitogenactivated protein kinase (ERK1/2). Dexamethasone 4-17 glucose-6-phosphate isomerase Homo sapiens 28-33 16269908-9 2005 RESULTS: In pHi experiments, we demonstrated that alcohol could induce a biphasic, concentration-dependent (30-1000 mM) pHi change (i.e., alkalosis after acidosis) in human atrium in HEPES-buffered Tyrode solution. Alcohols 50-57 glucose-6-phosphate isomerase Homo sapiens 12-15 16269908-9 2005 RESULTS: In pHi experiments, we demonstrated that alcohol could induce a biphasic, concentration-dependent (30-1000 mM) pHi change (i.e., alkalosis after acidosis) in human atrium in HEPES-buffered Tyrode solution. Alcohols 50-57 glucose-6-phosphate isomerase Homo sapiens 120-123 16269908-9 2005 RESULTS: In pHi experiments, we demonstrated that alcohol could induce a biphasic, concentration-dependent (30-1000 mM) pHi change (i.e., alkalosis after acidosis) in human atrium in HEPES-buffered Tyrode solution. HEPES 183-188 glucose-6-phosphate isomerase Homo sapiens 120-123 16269908-15 2005 All these alcohol-induced pHi changes and electromechanical inhibitions were reversible. Alcohols 10-17 glucose-6-phosphate isomerase Homo sapiens 26-29 16269908-16 2005 CONCLUSIONS: To our knowledge, this study provides the first evidence that alcohol can affect pHi in human myocardial tissue by changing the activity of acid extruders (i.e., NHE and NHS). Alcohols 75-82 glucose-6-phosphate isomerase Homo sapiens 94-97 16141236-7 2005 Heparan sulphate does not affect cell association of PGI/AMF at neutral pH but enhances the FN-independent cell surface association of PGI/AMF at acid pH identifying two distinct mechanisms for PGI/AMF sequestration under acidic conditions. Heparitin Sulfate 0-16 glucose-6-phosphate isomerase Homo sapiens 135-138 16141236-7 2005 Heparan sulphate does not affect cell association of PGI/AMF at neutral pH but enhances the FN-independent cell surface association of PGI/AMF at acid pH identifying two distinct mechanisms for PGI/AMF sequestration under acidic conditions. Heparitin Sulfate 0-16 glucose-6-phosphate isomerase Homo sapiens 139-142 16141236-7 2005 Heparan sulphate does not affect cell association of PGI/AMF at neutral pH but enhances the FN-independent cell surface association of PGI/AMF at acid pH identifying two distinct mechanisms for PGI/AMF sequestration under acidic conditions. Heparitin Sulfate 0-16 glucose-6-phosphate isomerase Homo sapiens 135-138 16141236-7 2005 Heparan sulphate does not affect cell association of PGI/AMF at neutral pH but enhances the FN-independent cell surface association of PGI/AMF at acid pH identifying two distinct mechanisms for PGI/AMF sequestration under acidic conditions. Heparitin Sulfate 0-16 glucose-6-phosphate isomerase Homo sapiens 139-142 15729714-5 2005 On the other hand, monensin, a Na+/H+ ionophore mimicking NHE activation, and phorbol 12-myristate 13-acetate (PMA), which stimulates NHE, significantly increased the pHi and decreased the drug accumulation in HT29 cells to values similar to those observed in control HT29-dx cells. Monensin 19-27 glucose-6-phosphate isomerase Homo sapiens 167-170 15729714-5 2005 On the other hand, monensin, a Na+/H+ ionophore mimicking NHE activation, and phorbol 12-myristate 13-acetate (PMA), which stimulates NHE, significantly increased the pHi and decreased the drug accumulation in HT29 cells to values similar to those observed in control HT29-dx cells. Tetradecanoylphorbol Acetate 78-109 glucose-6-phosphate isomerase Homo sapiens 167-170 15729714-5 2005 On the other hand, monensin, a Na+/H+ ionophore mimicking NHE activation, and phorbol 12-myristate 13-acetate (PMA), which stimulates NHE, significantly increased the pHi and decreased the drug accumulation in HT29 cells to values similar to those observed in control HT29-dx cells. Tetradecanoylphorbol Acetate 111-114 glucose-6-phosphate isomerase Homo sapiens 167-170 16229142-0 2005 A new role of glucose-6-phosphate isomerase: protection of cell structures from malonic dialdehyde. Malondialdehyde 80-98 glucose-6-phosphate isomerase Homo sapiens 14-43 15926041-3 2005 Intracellular pH (pH(i)) was measured spectrofluorometrically in primary cultured ruminal epithelial cells loaded with the pH-sensitive fluorescent dye, 2,7-bis(carboxyethyl)-5(6")-carboxyfluorescein acetomethyl ester. 2,7-bis(carboxyethyl)-5(6")-carboxyfluorescein acetomethyl ester 153-217 glucose-6-phosphate isomerase Homo sapiens 18-23 15926041-4 2005 Switching from CO2/HCO3- -buffered to HEPES-buffered solution caused a rapid intracellular alkalinization followed by a counter-regulation towards initial pH(i). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 15-18 glucose-6-phosphate isomerase Homo sapiens 155-160 15926041-4 2005 Switching from CO2/HCO3- -buffered to HEPES-buffered solution caused a rapid intracellular alkalinization followed by a counter-regulation towards initial pH(i). Bicarbonates 19-23 glucose-6-phosphate isomerase Homo sapiens 155-160 15926041-4 2005 Switching from CO2/HCO3- -buffered to HEPES-buffered solution caused a rapid intracellular alkalinization followed by a counter-regulation towards initial pH(i). HEPES 38-43 glucose-6-phosphate isomerase Homo sapiens 155-160 15926041-5 2005 The recovery of pH(i) was dependent upon extracellular chloride, but independent of extracellular sodium. Chlorides 55-63 glucose-6-phosphate isomerase Homo sapiens 16-21 15926041-6 2005 Adding 500 microM H2DIDS significantly reduced the increase of pH(i). dihydro-DIDS 18-24 glucose-6-phosphate isomerase Homo sapiens 63-68 15926041-8 2005 Under sodium and chloride-free conditions, counter-regulation after CO2-induced pH(i) decrease did not differ from pH(i) recovery in the presence of sodium and chloride. Chlorides 17-25 glucose-6-phosphate isomerase Homo sapiens 80-85 15926041-8 2005 Under sodium and chloride-free conditions, counter-regulation after CO2-induced pH(i) decrease did not differ from pH(i) recovery in the presence of sodium and chloride. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 68-71 glucose-6-phosphate isomerase Homo sapiens 80-85 16269908-7 2005 In the whole study, pHi was measured by an epifluorescent, ratiometric microspectrofluorimetry technique with the dye BCECF, while electrophysiological experiments were performed by traditional micropipette. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 118-123 glucose-6-phosphate isomerase Homo sapiens 20-23 16177028-10 2005 These data suggest that with single stimulation and during STN-BHFS, the STN-GPe excitatory response dominates over the STN-GPe-GPe recurrent inhibition in the GPe, whereas the STN-GPe-GPi inhibitory response dominates over the STN-GPi excitatory response in the GPi. glycyl-prolyl-glutamic acid 77-80 glucose-6-phosphate isomerase Homo sapiens 232-235 16177028-10 2005 These data suggest that with single stimulation and during STN-BHFS, the STN-GPe excitatory response dominates over the STN-GPe-GPe recurrent inhibition in the GPe, whereas the STN-GPe-GPi inhibitory response dominates over the STN-GPi excitatory response in the GPi. glycyl-prolyl-glutamic acid 77-80 glucose-6-phosphate isomerase Homo sapiens 232-235 16141236-0 2005 pH-specific sequestration of phosphoglucose isomerase/autocrine motility factor by fibronectin and heparan sulphate. Heparitin Sulfate 99-115 glucose-6-phosphate isomerase Homo sapiens 29-53 16141236-0 2005 pH-specific sequestration of phosphoglucose isomerase/autocrine motility factor by fibronectin and heparan sulphate. Heparitin Sulfate 99-115 glucose-6-phosphate isomerase Homo sapiens 54-79 16002403-7 2005 The pH sensitivity of CCE in HEK 293 cells is likely to dampen the effects of pH(i) on Ca2+-regulated ACs and may partly explain the discrepancy between in vitro and in vivo data. Carbamylcholine 22-25 glucose-6-phosphate isomerase Homo sapiens 78-83 16126909-1 2005 Phosphoglucose isomerase (PGI; EC 5.3.1.9) is a housekeeping cytosolic enzyme of the sugar metabolism pathways that plays a key role in glycolysis and gluconeogenesis. Sugars 85-90 glucose-6-phosphate isomerase Homo sapiens 0-24 16126909-1 2005 Phosphoglucose isomerase (PGI; EC 5.3.1.9) is a housekeeping cytosolic enzyme of the sugar metabolism pathways that plays a key role in glycolysis and gluconeogenesis. Sugars 85-90 glucose-6-phosphate isomerase Homo sapiens 26-29 15729714-2 2005 In our study, we have observed that the activity and expression of Na+/H+ exchanger (NHE), which is involved in the homeostasis of intracellular pH (pHi), are increased in doxorubicin-resistant (HT29-dx) human colon carcinoma cells in comparison with doxorubicin-sensitive HT29 cells. Doxorubicin 172-183 glucose-6-phosphate isomerase Homo sapiens 149-152 15729714-2 2005 In our study, we have observed that the activity and expression of Na+/H+ exchanger (NHE), which is involved in the homeostasis of intracellular pH (pHi), are increased in doxorubicin-resistant (HT29-dx) human colon carcinoma cells in comparison with doxorubicin-sensitive HT29 cells. Doxorubicin 251-262 glucose-6-phosphate isomerase Homo sapiens 149-152 15729714-4 2005 The NHE inhibitor 5-(N-ethyl-N-isopropyl)amiloride (EIPA) significantly reduced the pHi value and increased the intracellular accumulation of doxorubicin in both cell populations: in the presence of EIPA HT29-dx cells accumulated as much drug as control HT29 cells. ethylisopropylamiloride 18-50 glucose-6-phosphate isomerase Homo sapiens 84-87 15729714-4 2005 The NHE inhibitor 5-(N-ethyl-N-isopropyl)amiloride (EIPA) significantly reduced the pHi value and increased the intracellular accumulation of doxorubicin in both cell populations: in the presence of EIPA HT29-dx cells accumulated as much drug as control HT29 cells. ethylisopropylamiloride 52-56 glucose-6-phosphate isomerase Homo sapiens 84-87 15856280-4 2005 Under experimental conditions that reduce the intrinsic Na/H exchanger activity, exposure of control cells to a 10 mM NH4Cl- containing solution induces the classic pHi response profile of cells having a high permeability to NH3 (PNH3) but relatively low permeability to NH4+ (PNH4). Ammonia 225-228 glucose-6-phosphate isomerase Homo sapiens 165-168 15856280-4 2005 Under experimental conditions that reduce the intrinsic Na/H exchanger activity, exposure of control cells to a 10 mM NH4Cl- containing solution induces the classic pHi response profile of cells having a high permeability to NH3 (PNH3) but relatively low permeability to NH4+ (PNH4). pnh3 230-234 glucose-6-phosphate isomerase Homo sapiens 165-168 15856280-4 2005 Under experimental conditions that reduce the intrinsic Na/H exchanger activity, exposure of control cells to a 10 mM NH4Cl- containing solution induces the classic pHi response profile of cells having a high permeability to NH3 (PNH3) but relatively low permeability to NH4+ (PNH4). Ammonium Compounds 271-275 glucose-6-phosphate isomerase Homo sapiens 165-168 15856280-4 2005 Under experimental conditions that reduce the intrinsic Na/H exchanger activity, exposure of control cells to a 10 mM NH4Cl- containing solution induces the classic pHi response profile of cells having a high permeability to NH3 (PNH3) but relatively low permeability to NH4+ (PNH4). pnh4 277-281 glucose-6-phosphate isomerase Homo sapiens 165-168 15856280-6 2005 Measurements of pHi during methylammonium exposure showed that RhAG expression enhances the influx of both the unprotonated and ionic forms of methylammonium. methylamine 27-41 glucose-6-phosphate isomerase Homo sapiens 16-19 15856280-6 2005 Measurements of pHi during methylammonium exposure showed that RhAG expression enhances the influx of both the unprotonated and ionic forms of methylammonium. methylamine 143-157 glucose-6-phosphate isomerase Homo sapiens 16-19 15896063-1 2005 Mammalian alkaline phosphatases (AP) are glycosylphosphatidylinositol (GPI) anchored proteins that are localized on the outer layer of the plasma membrane. Glycosylphosphatidylinositols 41-69 glucose-6-phosphate isomerase Homo sapiens 71-74 15896063-2 2005 The GPI anchors are covalently attached to the C-termini of proteins and consist of a glycan chain bonded to phosphatidylinositol with two acyl chains anchored into the membrane bilayer. Polysaccharides 86-92 glucose-6-phosphate isomerase Homo sapiens 4-7 15896063-2 2005 The GPI anchors are covalently attached to the C-termini of proteins and consist of a glycan chain bonded to phosphatidylinositol with two acyl chains anchored into the membrane bilayer. Phosphatidylinositols 109-129 glucose-6-phosphate isomerase Homo sapiens 4-7 15896063-5 2005 An adhesion force of 350 +/- 200 pN is measured between GPI-anchored AP (AP(GPI)) and supported phospholipid bilayers of dipalmitoylphosphatidylcholine (DPPC) presenting structural defects (holes). Phospholipids 96-108 glucose-6-phosphate isomerase Homo sapiens 73-80 15637053-1 2005 Phosphoglucose isomerase (PGI; EC 5.3.1.9) is a cytosolic housekeeping enzyme of the sugar metabolism pathways that plays a key role in both glycolysis and gluconeogenesis. Sugars 85-90 glucose-6-phosphate isomerase Homo sapiens 0-24 16852150-9 2005 Although the XPS data shows carbon present at the Ag(111) surface after exposure to PhI, no features attributable to phenyl groups were observed by STM. Carbon 28-34 glucose-6-phosphate isomerase Homo sapiens 84-87 15601755-4 2005 Acidification with the K+/H+ exchanger nigericin reduced pHi to 6.25+/-0.15 (without HCO3-) or 6.53+/-0.10 (with HCO3-). Nigericin 39-48 glucose-6-phosphate isomerase Homo sapiens 57-60 15850830-13 2005 The addition of an HIF-1 inhibitor, cadmium chloride (CdCl2), or alpha-ketoglutarate (2-oxoglutarate) decreased the AMF mRNA expression, and an AMF inhibitor, erythrose 4-phosphate, decreased the cell motility. Cadmium Chloride 36-52 glucose-6-phosphate isomerase Homo sapiens 116-119 15850830-13 2005 The addition of an HIF-1 inhibitor, cadmium chloride (CdCl2), or alpha-ketoglutarate (2-oxoglutarate) decreased the AMF mRNA expression, and an AMF inhibitor, erythrose 4-phosphate, decreased the cell motility. Cadmium Chloride 36-52 glucose-6-phosphate isomerase Homo sapiens 144-147 15850830-13 2005 The addition of an HIF-1 inhibitor, cadmium chloride (CdCl2), or alpha-ketoglutarate (2-oxoglutarate) decreased the AMF mRNA expression, and an AMF inhibitor, erythrose 4-phosphate, decreased the cell motility. Cadmium Chloride 54-59 glucose-6-phosphate isomerase Homo sapiens 116-119 15850830-13 2005 The addition of an HIF-1 inhibitor, cadmium chloride (CdCl2), or alpha-ketoglutarate (2-oxoglutarate) decreased the AMF mRNA expression, and an AMF inhibitor, erythrose 4-phosphate, decreased the cell motility. Ketoglutaric Acids 65-84 glucose-6-phosphate isomerase Homo sapiens 116-119 15850830-13 2005 The addition of an HIF-1 inhibitor, cadmium chloride (CdCl2), or alpha-ketoglutarate (2-oxoglutarate) decreased the AMF mRNA expression, and an AMF inhibitor, erythrose 4-phosphate, decreased the cell motility. Ketoglutaric Acids 86-100 glucose-6-phosphate isomerase Homo sapiens 116-119 15850830-13 2005 The addition of an HIF-1 inhibitor, cadmium chloride (CdCl2), or alpha-ketoglutarate (2-oxoglutarate) decreased the AMF mRNA expression, and an AMF inhibitor, erythrose 4-phosphate, decreased the cell motility. erythrose 4-phosphate 159-180 glucose-6-phosphate isomerase Homo sapiens 144-147 15637053-1 2005 Phosphoglucose isomerase (PGI; EC 5.3.1.9) is a cytosolic housekeeping enzyme of the sugar metabolism pathways that plays a key role in both glycolysis and gluconeogenesis. Sugars 85-90 glucose-6-phosphate isomerase Homo sapiens 26-29 15637053-3 2005 Little is known of the biochemical regulation of PGI/AMF activities, although it is known that human PGI/AMF is phosphorylated at Ser(185) by protein kinase CK2 (CK2); however, the physiological significance of this phosphorylation is unknown. Serine 130-133 glucose-6-phosphate isomerase Homo sapiens 101-104 15637053-3 2005 Little is known of the biochemical regulation of PGI/AMF activities, although it is known that human PGI/AMF is phosphorylated at Ser(185) by protein kinase CK2 (CK2); however, the physiological significance of this phosphorylation is unknown. Serine 130-133 glucose-6-phosphate isomerase Homo sapiens 105-108 15637053-12 2005 The process by which phosphorylation modulates the enzymatic activity of PGI thus has an important implication for the understanding of the biological regulation of this key glucose metabolism-regulating enzyme. Glucose 174-181 glucose-6-phosphate isomerase Homo sapiens 73-76 15654389-1 2005 A new [2 + 2 + 2] approach to construct GPI molecules through the efficient synthesis of glucosamine-inositol and tetramannose intermediates led to a total synthesis of a GPI-anchor of Trypanosoma cruzi, and also afforded a key intermediate for the synthesis of valuable [4-deoxy-Man-III]-GPI analogues. glucosamine-inositol 89-109 glucose-6-phosphate isomerase Homo sapiens 40-43 15728055-0 2005 Sedation with GPI 15715, a water-soluble prodrug of propofol, using target-controlled infusion in volunteers. Propofol 52-60 glucose-6-phosphate isomerase Homo sapiens 14-17 15728055-1 2005 GPI 15715 is the first water-soluble propofol prodrug that has been studied in humans. Water 23-28 glucose-6-phosphate isomerase Homo sapiens 0-3 15728055-1 2005 GPI 15715 is the first water-soluble propofol prodrug that has been studied in humans. Propofol 37-45 glucose-6-phosphate isomerase Homo sapiens 0-3 15886061-5 2005 However, compared to glibenclamide-sensitive cells, stationary phase resistant parasites display an increased use of amino acids as energy substrate and an increased activity of the enzymes hexokinase, phosphoglucose isomerase, and especially NAD(+)-linked glutamate dehydrogenase. Glyburide 21-34 glucose-6-phosphate isomerase Homo sapiens 202-226 15701437-4 2005 Patients were analyzed according to donor-recipient weight ratio (D-R): Group (Gp) I (n = 252) with D-R <2.5 (range 0.59 to 2.49, median 1.4), and Gp II (n = 39) with D-R >/=2.5 (range 2.5 to 4.65, median 2.78). Leu-Thr 105-107 glucose-6-phosphate isomerase Homo sapiens 72-84 15701437-4 2005 Patients were analyzed according to donor-recipient weight ratio (D-R): Group (Gp) I (n = 252) with D-R <2.5 (range 0.59 to 2.49, median 1.4), and Gp II (n = 39) with D-R >/=2.5 (range 2.5 to 4.65, median 2.78). Asp-Arg 100-103 glucose-6-phosphate isomerase Homo sapiens 72-84 15654389-1 2005 A new [2 + 2 + 2] approach to construct GPI molecules through the efficient synthesis of glucosamine-inositol and tetramannose intermediates led to a total synthesis of a GPI-anchor of Trypanosoma cruzi, and also afforded a key intermediate for the synthesis of valuable [4-deoxy-Man-III]-GPI analogues. glucosamine-inositol 89-109 glucose-6-phosphate isomerase Homo sapiens 171-174 15654389-1 2005 A new [2 + 2 + 2] approach to construct GPI molecules through the efficient synthesis of glucosamine-inositol and tetramannose intermediates led to a total synthesis of a GPI-anchor of Trypanosoma cruzi, and also afforded a key intermediate for the synthesis of valuable [4-deoxy-Man-III]-GPI analogues. glucosamine-inositol 89-109 glucose-6-phosphate isomerase Homo sapiens 171-174 15654389-1 2005 A new [2 + 2 + 2] approach to construct GPI molecules through the efficient synthesis of glucosamine-inositol and tetramannose intermediates led to a total synthesis of a GPI-anchor of Trypanosoma cruzi, and also afforded a key intermediate for the synthesis of valuable [4-deoxy-Man-III]-GPI analogues. tetramannose 114-126 glucose-6-phosphate isomerase Homo sapiens 40-43 15654389-1 2005 A new [2 + 2 + 2] approach to construct GPI molecules through the efficient synthesis of glucosamine-inositol and tetramannose intermediates led to a total synthesis of a GPI-anchor of Trypanosoma cruzi, and also afforded a key intermediate for the synthesis of valuable [4-deoxy-Man-III]-GPI analogues. tetramannose 114-126 glucose-6-phosphate isomerase Homo sapiens 171-174 15654389-1 2005 A new [2 + 2 + 2] approach to construct GPI molecules through the efficient synthesis of glucosamine-inositol and tetramannose intermediates led to a total synthesis of a GPI-anchor of Trypanosoma cruzi, and also afforded a key intermediate for the synthesis of valuable [4-deoxy-Man-III]-GPI analogues. tetramannose 114-126 glucose-6-phosphate isomerase Homo sapiens 171-174 15850610-11 2005 The increase of the decline in pHi elicited by preexposure to thrombin was still observed in the presence of an inhibitor of the Na+/H+ exchange or in sodium-free solutions. Sodium 151-157 glucose-6-phosphate isomerase Homo sapiens 31-34 15667798-12 2005 The pHi in Group N + E was maintained above 7.35 during OPCABG, while it was less than 7.35 from T4 to T7 in Group X + E. CONCLUSION: Nicardipine combined with esmolol (1:10) regimen may maintain systemic and tissue oxygenation during OPCABG. Nicardipine 134-145 glucose-6-phosphate isomerase Homo sapiens 4-7 15779865-1 2005 We had reported that the intracellular pH (pHi) of human lung adenocarcinoma A549 cells, which is sensitive to cisplatin, was more acidic than that of cisplatin-resistant A549/DDP cells. Cisplatin 111-120 glucose-6-phosphate isomerase Homo sapiens 43-46 15779865-1 2005 We had reported that the intracellular pH (pHi) of human lung adenocarcinoma A549 cells, which is sensitive to cisplatin, was more acidic than that of cisplatin-resistant A549/DDP cells. Cisplatin 151-160 glucose-6-phosphate isomerase Homo sapiens 43-46 15779865-2 2005 The correlation between the change of the pHi and cisplatin-resistance of A549/DDP cells was further studied by altering pHi in consequence of the change of CO2 concentration of the incubator. Cisplatin 50-59 glucose-6-phosphate isomerase Homo sapiens 42-45 15779865-2 2005 The correlation between the change of the pHi and cisplatin-resistance of A549/DDP cells was further studied by altering pHi in consequence of the change of CO2 concentration of the incubator. Cisplatin 50-59 glucose-6-phosphate isomerase Homo sapiens 121-124 15779865-2 2005 The correlation between the change of the pHi and cisplatin-resistance of A549/DDP cells was further studied by altering pHi in consequence of the change of CO2 concentration of the incubator. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 157-160 glucose-6-phosphate isomerase Homo sapiens 121-124 15779865-4 2005 The results indicated that the pHi was more alkaline at lower CO2 concentration (2% CO2 in the incubator) and more acidic at higher CO2 concentration (8% CO2 in the incubator) for both A549 and A549/DDP cells compared with those of both A549 and A549/DDP cells cultured at 5% CO2 as the normal condition. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 62-65 glucose-6-phosphate isomerase Homo sapiens 31-34 15779865-4 2005 The results indicated that the pHi was more alkaline at lower CO2 concentration (2% CO2 in the incubator) and more acidic at higher CO2 concentration (8% CO2 in the incubator) for both A549 and A549/DDP cells compared with those of both A549 and A549/DDP cells cultured at 5% CO2 as the normal condition. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 84-87 glucose-6-phosphate isomerase Homo sapiens 31-34 15779865-4 2005 The results indicated that the pHi was more alkaline at lower CO2 concentration (2% CO2 in the incubator) and more acidic at higher CO2 concentration (8% CO2 in the incubator) for both A549 and A549/DDP cells compared with those of both A549 and A549/DDP cells cultured at 5% CO2 as the normal condition. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 84-87 glucose-6-phosphate isomerase Homo sapiens 31-34 15779865-4 2005 The results indicated that the pHi was more alkaline at lower CO2 concentration (2% CO2 in the incubator) and more acidic at higher CO2 concentration (8% CO2 in the incubator) for both A549 and A549/DDP cells compared with those of both A549 and A549/DDP cells cultured at 5% CO2 as the normal condition. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 84-87 glucose-6-phosphate isomerase Homo sapiens 31-34 15779865-4 2005 The results indicated that the pHi was more alkaline at lower CO2 concentration (2% CO2 in the incubator) and more acidic at higher CO2 concentration (8% CO2 in the incubator) for both A549 and A549/DDP cells compared with those of both A549 and A549/DDP cells cultured at 5% CO2 as the normal condition. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 84-87 glucose-6-phosphate isomerase Homo sapiens 31-34 15779865-8 2005 It is proposed from the above results that the change of pHi in especially more acidic compartments in A549/DDP cells involves their cisplatin resistance. Cisplatin 133-142 glucose-6-phosphate isomerase Homo sapiens 57-60 15675608-1 2005 GPI-15715 (PQ-1002, Aquavan), a water-soluble prodrug of propofol, is being developed by Guilford, under license from ProQuest, as a potential anesthetic agent. Fospropofol disodium 11-18 glucose-6-phosphate isomerase Homo sapiens 0-3 15675608-1 2005 GPI-15715 (PQ-1002, Aquavan), a water-soluble prodrug of propofol, is being developed by Guilford, under license from ProQuest, as a potential anesthetic agent. Water 32-37 glucose-6-phosphate isomerase Homo sapiens 0-3 15675608-1 2005 GPI-15715 (PQ-1002, Aquavan), a water-soluble prodrug of propofol, is being developed by Guilford, under license from ProQuest, as a potential anesthetic agent. Propofol 57-65 glucose-6-phosphate isomerase Homo sapiens 0-3 15850610-3 2005 On resuspension of BCECF-loaded platelets in a chloride-free medium (gluconate replaced) that contains bicarbonate, cytosolic pH (pHi) increased and stabilized after 10 min at an alkaline value. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 19-24 glucose-6-phosphate isomerase Homo sapiens 130-133 15850610-3 2005 On resuspension of BCECF-loaded platelets in a chloride-free medium (gluconate replaced) that contains bicarbonate, cytosolic pH (pHi) increased and stabilized after 10 min at an alkaline value. gluconic acid 69-78 glucose-6-phosphate isomerase Homo sapiens 130-133 15850610-3 2005 On resuspension of BCECF-loaded platelets in a chloride-free medium (gluconate replaced) that contains bicarbonate, cytosolic pH (pHi) increased and stabilized after 10 min at an alkaline value. Bicarbonates 103-114 glucose-6-phosphate isomerase Homo sapiens 130-133 15850610-4 2005 After addition of 50 mM NaCl, pHi fell rapidly reaching steady state in the succeeding 5 min. Sodium Chloride 24-28 glucose-6-phosphate isomerase Homo sapiens 30-33 15558024-2 2005 In this study, we examined whether amiloride, an inhibitor of the Na(+)/H(+) antiporter capable of lowering the intracellular pH (pHi), can potentiate TRAIL-induced apoptotic death. Amiloride 35-44 glucose-6-phosphate isomerase Homo sapiens 130-133 15478002-5 2005 The results of these studies revealed that the inhibition of either N-linked glycosylation or oligosaccharide synthesis for GPI-anchor addition could affect the synthesis and the distribution of sALP, but not the kinetics of the phosphatase reaction. Nitrogen 68-69 glucose-6-phosphate isomerase Homo sapiens 124-127 15478002-5 2005 The results of these studies revealed that the inhibition of either N-linked glycosylation or oligosaccharide synthesis for GPI-anchor addition could affect the synthesis and the distribution of sALP, but not the kinetics of the phosphatase reaction. Oligosaccharides 94-109 glucose-6-phosphate isomerase Homo sapiens 124-127 15478002-8 2005 In contrast to the effects of tunicamycin on N-linked glycosylation, the effects of mannosamine, which inhibits GPI-anchor glycosylation/formation, included (1) an increase in cell layer protein; (2) decreases in sALP specific activity, in the cells and in the CM; and (3) increases in the percentages of both anchorless and wheat germ agglutinin (WGA)-soluble sALP in the medium, but not in the cells (P <0.005 for each). mannosamine 84-95 glucose-6-phosphate isomerase Homo sapiens 112-115 16594477-2 2005 The technique utilizes the PCO2 of the gastric fluid to calculate intramucosal pH (pHi). pco2 27-31 glucose-6-phosphate isomerase Homo sapiens 83-86 15803754-6 2004 Our results indicated that (1) LPS-stimulated pHi elevation was PKC dependent; (2) After 30 min stimulation, LPS increased PLD activity via a measured production of 3H-phosphatidylethanol from phosphatidic acid and the initiation of PLD1a mRNA expression started; (2) LPS stimulated IL-2 R expression but not IL-2 and IL-4 secretion. 3h-phosphatidylethanol 165-187 glucose-6-phosphate isomerase Homo sapiens 46-49 15850610-6 2005 The decline in pHi was observed whether chloride was delivered to the solution in the form of LiCl or NaCl, or when the later was applied after blockage of the Na+/H+ exchanger. Chlorides 40-48 glucose-6-phosphate isomerase Homo sapiens 15-18 15850610-6 2005 The decline in pHi was observed whether chloride was delivered to the solution in the form of LiCl or NaCl, or when the later was applied after blockage of the Na+/H+ exchanger. Lithium Chloride 94-98 glucose-6-phosphate isomerase Homo sapiens 15-18 15850610-6 2005 The decline in pHi was observed whether chloride was delivered to the solution in the form of LiCl or NaCl, or when the later was applied after blockage of the Na+/H+ exchanger. Sodium Chloride 102-106 glucose-6-phosphate isomerase Homo sapiens 15-18 15850610-8 2005 Posterior addition of NaCl after 5 min in high gluconate reproduced the pHi fall of the control experiment. Sodium Chloride 22-26 glucose-6-phosphate isomerase Homo sapiens 72-75 15850610-8 2005 Posterior addition of NaCl after 5 min in high gluconate reproduced the pHi fall of the control experiment. gluconic acid 47-56 glucose-6-phosphate isomerase Homo sapiens 72-75 15850610-12 2005 It is concluded that a Na-independent Cl-/HCO3- exchange mechanism mediates the recovery of pHi from alkalosis in platelets and that thrombin activates this exchanger by a direct regulatory pathway. Bicarbonates 42-46 glucose-6-phosphate isomerase Homo sapiens 92-95 15306540-2 2004 These channels are blocked by nickel, inactivate in 1-2 min in calcium-deprived medium, and are remarkably stimulated by NH(4)Cl, suggesting a role for intracellular pH (pH(i)). Nickel 30-36 glucose-6-phosphate isomerase Homo sapiens 170-175 15355852-2 2004 Extracellular ATP reversibly induced a rapid and sustained intracellular pH (pH(i)) decrease from 7.41 to 7.11. Adenosine Triphosphate 14-17 glucose-6-phosphate isomerase Homo sapiens 77-82 15733483-1 2004 OBJECTIVE: To find out the connection of serum pepsinogen and it"s subgroups (PGI, PGII) with CA72-4 to early diagnosis and postoperative recurrence on gastric cancer. ca72-4 94-100 glucose-6-phosphate isomerase Homo sapiens 78-81 15733483-10 2004 CONCLUSIONS: Apply to detect the serum PG levels on crowds, especially pGI, PGI/II levels decrease, which may be expected to become the index to earlier period GC screening. pg 39-41 glucose-6-phosphate isomerase Homo sapiens 71-74 15733483-10 2004 CONCLUSIONS: Apply to detect the serum PG levels on crowds, especially pGI, PGI/II levels decrease, which may be expected to become the index to earlier period GC screening. pg 39-41 glucose-6-phosphate isomerase Homo sapiens 76-79 15306540-2 2004 These channels are blocked by nickel, inactivate in 1-2 min in calcium-deprived medium, and are remarkably stimulated by NH(4)Cl, suggesting a role for intracellular pH (pH(i)). Calcium 63-70 glucose-6-phosphate isomerase Homo sapiens 170-175 15306540-2 2004 These channels are blocked by nickel, inactivate in 1-2 min in calcium-deprived medium, and are remarkably stimulated by NH(4)Cl, suggesting a role for intracellular pH (pH(i)). Ammonium Chloride 121-128 glucose-6-phosphate isomerase Homo sapiens 170-175 15306540-5 2004 Simultaneous depolarization and pH(i) alkalinization with NH(4)Cl induced an [Ca(2+)](i) increase that depended on the amount of NH(4)Cl added. Ammonium Chloride 58-65 glucose-6-phosphate isomerase Homo sapiens 32-37 15306540-5 2004 Simultaneous depolarization and pH(i) alkalinization with NH(4)Cl induced an [Ca(2+)](i) increase that depended on the amount of NH(4)Cl added. Ammonium Chloride 129-136 glucose-6-phosphate isomerase Homo sapiens 32-37 15306540-9 2004 The ratio of pH(i)-stimulated-to-nonstimulated calcium influx was nearly constant at different test depolarization values. Calcium 47-54 glucose-6-phosphate isomerase Homo sapiens 13-18 15306540-10 2004 Likewise, depolarization-induced calcium influx in pH(i)-stimulated and nonstimulated cells was equally blocked by nickel. Calcium 33-40 glucose-6-phosphate isomerase Homo sapiens 51-56 15306540-10 2004 Likewise, depolarization-induced calcium influx in pH(i)-stimulated and nonstimulated cells was equally blocked by nickel. Nickel 115-121 glucose-6-phosphate isomerase Homo sapiens 51-56 15306540-11 2004 In our capacitating conditions pH(i) increased 0.11 pH units, suggesting that the calcium influx stimulation observed during sperm capacitation might be partially caused by pH(i) alkalinization. Calcium 82-89 glucose-6-phosphate isomerase Homo sapiens 31-36 15306540-11 2004 In our capacitating conditions pH(i) increased 0.11 pH units, suggesting that the calcium influx stimulation observed during sperm capacitation might be partially caused by pH(i) alkalinization. Calcium 82-89 glucose-6-phosphate isomerase Homo sapiens 173-178 15306540-12 2004 Additionally, a calcium permeability pathway triggered exclusively by pH(i) alkalinization was detected. Calcium 16-23 glucose-6-phosphate isomerase Homo sapiens 70-75 15197238-1 2004 Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal, acquired hematopoietic disorder characterized by a phosphatidylinositol (PI) glycan-A gene mutation, which impairs the synthesis of the glycosyl-PI (GPI) anchor, thus causing the absence of all GPI-linked proteins on the membrane of the clonal-defective cells. Phosphatidylinositols 106-126 glucose-6-phosphate isomerase Homo sapiens 191-202 15452108-3 2004 4,4"-Diisothiocyanostilbene-2,2"-disulfonic acid (DIDS)-sensitive 36Cl- efflux from AE2-expressing Xenopus oocytes was monitored during changes in pHi or pHo in HEPES-buffered and in 5% CO2/HCO3- -buffered conditions. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 0-48 glucose-6-phosphate isomerase Homo sapiens 147-150 15452108-3 2004 4,4"-Diisothiocyanostilbene-2,2"-disulfonic acid (DIDS)-sensitive 36Cl- efflux from AE2-expressing Xenopus oocytes was monitored during changes in pHi or pHo in HEPES-buffered and in 5% CO2/HCO3- -buffered conditions. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 50-54 glucose-6-phosphate isomerase Homo sapiens 147-150 15452108-3 2004 4,4"-Diisothiocyanostilbene-2,2"-disulfonic acid (DIDS)-sensitive 36Cl- efflux from AE2-expressing Xenopus oocytes was monitored during changes in pHi or pHo in HEPES-buffered and in 5% CO2/HCO3- -buffered conditions. Chlorine-36 66-70 glucose-6-phosphate isomerase Homo sapiens 147-150 15613449-11 2004 The anti-apoptotic effect of AMF has been described by other authors who have shown that the AMF over-expressing cells were resistant to mitomycin-C-induced apoptosis showing regression of Apaf-1 and caspase-9 dependent on PI3K and MAP kinase. Mitomycin 137-148 glucose-6-phosphate isomerase Homo sapiens 29-32 15613449-11 2004 The anti-apoptotic effect of AMF has been described by other authors who have shown that the AMF over-expressing cells were resistant to mitomycin-C-induced apoptosis showing regression of Apaf-1 and caspase-9 dependent on PI3K and MAP kinase. Mitomycin 137-148 glucose-6-phosphate isomerase Homo sapiens 93-96 15308676-4 2004 Alkalization by ammonium pre-pulse to pHi 7.78 +/- 0.02 resulted in a 2.2 +/- 0.1 Hz CBF increase (P < 0.05). Ammonium Compounds 16-24 glucose-6-phosphate isomerase Homo sapiens 38-41 15308676-5 2004 Following removal of NH4Cl, pHi decreased to 7.24 +/- 0.02 and CBF to 5.8 +/- 0.1 Hz (P < 0.05). Ammonium Chloride 21-26 glucose-6-phosphate isomerase Homo sapiens 28-31 15308676-6 2004 Removal of extracellular CO2 to change pHi resulted in similar CBF changes. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 25-28 glucose-6-phosphate isomerase Homo sapiens 39-42 15382148-4 2004 Flow cytometry was used to determine pHi with 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester or 5-(and-6)-carboxy SNARF-1 acetoxymethyl ester acetate, and the appropriate fluorescence ratios were measured. 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester 46-121 glucose-6-phosphate isomerase Homo sapiens 37-40 15382148-5 2004 The calibration of fluorescence ratios versus pHi was established by using ionophore nigericin. Nigericin 85-94 glucose-6-phosphate isomerase Homo sapiens 46-49 15382148-6 2004 The activity of NHE was calculated by a kinetic flow cytometric assay as the slope at time 0 of the best-fit curve of pHi recovery versus time after intracellular acidification with a pulse of exogenous sodium propionate. sodium propionate 203-220 glucose-6-phosphate isomerase Homo sapiens 118-121 15470093-2 2004 Assembly of Saccharomyces cerevisiae GPIs includes the addition of a fourth, side-branching mannose (Man) to the third Man of the core GPI glycan by the Smp3 mannosyltransferase. Mannose 92-99 glucose-6-phosphate isomerase Homo sapiens 37-40 15470093-2 2004 Assembly of Saccharomyces cerevisiae GPIs includes the addition of a fourth, side-branching mannose (Man) to the third Man of the core GPI glycan by the Smp3 mannosyltransferase. Polysaccharides 139-145 glucose-6-phosphate isomerase Homo sapiens 37-40 15470093-6 2004 Repression of CaSMP3 expression leads to accumulation of a GPI precursor glycolipid whose glycan headgroup contains three mannoses and bears a phosphodiester-linked substituent on its first Man. Glycolipids 73-83 glucose-6-phosphate isomerase Homo sapiens 59-62 15470093-6 2004 Repression of CaSMP3 expression leads to accumulation of a GPI precursor glycolipid whose glycan headgroup contains three mannoses and bears a phosphodiester-linked substituent on its first Man. Polysaccharides 90-96 glucose-6-phosphate isomerase Homo sapiens 59-62 15470093-6 2004 Repression of CaSMP3 expression leads to accumulation of a GPI precursor glycolipid whose glycan headgroup contains three mannoses and bears a phosphodiester-linked substituent on its first Man. Mannose 122-130 glucose-6-phosphate isomerase Homo sapiens 59-62 15329587-0 2004 Comparative pharmacokinetics and pharmacodynamics of the new propofol prodrug GPI 15715 and propofol emulsion. Propofol 61-69 glucose-6-phosphate isomerase Homo sapiens 78-81 15329587-1 2004 BACKGROUND: GPI 15715 is a new water-soluble prodrug that is hydrolyzed to release propofol. Water 31-36 glucose-6-phosphate isomerase Homo sapiens 12-15 15329587-1 2004 BACKGROUND: GPI 15715 is a new water-soluble prodrug that is hydrolyzed to release propofol. Propofol 83-91 glucose-6-phosphate isomerase Homo sapiens 12-15 15329587-8 2004 RESULTS: Compared with propofol emulsion, propofol from GPI 15715 showed a different disposition function and especially larger volumes of distribution. Propofol 42-50 glucose-6-phosphate isomerase Homo sapiens 56-59 15329587-9 2004 The propofol effect site concentration for half maximum effect was 2.0 +/- 0.5 microg/ml for GPI 15715 and 3.0 +/- 0.7 microg/ml for propofol emulsion (P < 0.05). Propofol 4-12 glucose-6-phosphate isomerase Homo sapiens 93-96 15329587-10 2004 Propofol from GPI 15715 did not show a hysteresis between plasma concentration and effect. Propofol 0-8 glucose-6-phosphate isomerase Homo sapiens 14-17 15329587-11 2004 CONCLUSIONS: Compared with propofol emulsion, propofol from GPI 15715 showed different pharmacokinetics and pharmacodynamics, particularly a higher potency with respect to concentration. Propofol 46-54 glucose-6-phosphate isomerase Homo sapiens 60-63 15372609-1 2004 We report on a 66-year-old woman in whom GPi pallidotomy produced progressive and eventually complete relief of hemichorea/ballism (HCB) after a subthalamic hemorrhage. Hexachlorobenzene 132-135 glucose-6-phosphate isomerase Homo sapiens 41-44 15197238-1 2004 Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal, acquired hematopoietic disorder characterized by a phosphatidylinositol (PI) glycan-A gene mutation, which impairs the synthesis of the glycosyl-PI (GPI) anchor, thus causing the absence of all GPI-linked proteins on the membrane of the clonal-defective cells. Phosphatidylinositols 106-126 glucose-6-phosphate isomerase Homo sapiens 204-207 15197238-1 2004 Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal, acquired hematopoietic disorder characterized by a phosphatidylinositol (PI) glycan-A gene mutation, which impairs the synthesis of the glycosyl-PI (GPI) anchor, thus causing the absence of all GPI-linked proteins on the membrane of the clonal-defective cells. Phosphatidylinositols 106-126 glucose-6-phosphate isomerase Homo sapiens 249-252 15278148-1 2004 Ligandless palladium-catalyzed Suzuki-Miyaura coupling converted an inert p-bromobenzyl ether to a DDQ-labile p-(3,4-dimethoxyphenyl) benzyl ether in the presence of azide functionality and this strategy serves as a key step for the convergent synthesis of a fully lipidated malaria GPI disaccharide. p-bromobenzyl ether 74-93 glucose-6-phosphate isomerase Homo sapiens 283-286 15278148-1 2004 Ligandless palladium-catalyzed Suzuki-Miyaura coupling converted an inert p-bromobenzyl ether to a DDQ-labile p-(3,4-dimethoxyphenyl) benzyl ether in the presence of azide functionality and this strategy serves as a key step for the convergent synthesis of a fully lipidated malaria GPI disaccharide. dichlorodicyanobenzoquinone 99-102 glucose-6-phosphate isomerase Homo sapiens 283-286 15278148-1 2004 Ligandless palladium-catalyzed Suzuki-Miyaura coupling converted an inert p-bromobenzyl ether to a DDQ-labile p-(3,4-dimethoxyphenyl) benzyl ether in the presence of azide functionality and this strategy serves as a key step for the convergent synthesis of a fully lipidated malaria GPI disaccharide. Palladium 11-20 glucose-6-phosphate isomerase Homo sapiens 283-286 15278148-1 2004 Ligandless palladium-catalyzed Suzuki-Miyaura coupling converted an inert p-bromobenzyl ether to a DDQ-labile p-(3,4-dimethoxyphenyl) benzyl ether in the presence of azide functionality and this strategy serves as a key step for the convergent synthesis of a fully lipidated malaria GPI disaccharide. p-(3,4-dimethoxyphenyl) benzyl ether 110-146 glucose-6-phosphate isomerase Homo sapiens 283-286 15278148-1 2004 Ligandless palladium-catalyzed Suzuki-Miyaura coupling converted an inert p-bromobenzyl ether to a DDQ-labile p-(3,4-dimethoxyphenyl) benzyl ether in the presence of azide functionality and this strategy serves as a key step for the convergent synthesis of a fully lipidated malaria GPI disaccharide. Azides 166-171 glucose-6-phosphate isomerase Homo sapiens 283-286 15262231-5 2004 PGI-LysAP has a pI around 6.0 and high specificity toward the synthetic, fluorogenic substrate l-lysyl-7-amino-4-methylcoumarin. l-lysyl-7-amino-4-methylcoumarin 95-127 glucose-6-phosphate isomerase Homo sapiens 0-3 15219861-3 2004 [D-1-Nal3]Morphiceptin was the most potent analog of this series with a 26-fold increase in mu-opioid receptor affinity, a 15-fold potency increase in the GPI assay, and a significant potency increase in the hot-plate analgesic test, as compared with morphiceptin. [d-1-nal3]morphiceptin 0-22 glucose-6-phosphate isomerase Homo sapiens 155-158 15219861-4 2004 [d-Qal3]Morphiceptin was found to be a weak antagonist in the GPI assay. [d-qal3]morphiceptin 0-20 glucose-6-phosphate isomerase Homo sapiens 62-65 15103138-1 2004 Glucose-6-phosphate isomerase (PGI; EC 5.3.1.9; also often called by its old nomenclature phosphoglucose isomerase) is an intracellular enzyme that catalyses the reversible conversion of D-glucose 6-phosphate (G6P) to D-fructose 6-phosphate (F6P). Glucose-6-Phosphate 187-208 glucose-6-phosphate isomerase Homo sapiens 31-34 15197104-1 2004 Topiramate (TPM) is an anticonvulsant whose impact on firing activity and intracellular pH (pHi) regulation of CA3 neurons was investigated. Topiramate 0-10 glucose-6-phosphate isomerase Homo sapiens 92-95 15197104-1 2004 Topiramate (TPM) is an anticonvulsant whose impact on firing activity and intracellular pH (pHi) regulation of CA3 neurons was investigated. Topiramate 12-15 glucose-6-phosphate isomerase Homo sapiens 92-95 15197104-11 2004 However, pHi recovery subsequent to an ammonium prepulse was slightly increased, as was the case in the presence of the carbonic anhydrase (CA) inhibitor acetazolamide. Ammonium Compounds 39-47 glucose-6-phosphate isomerase Homo sapiens 9-12 15010500-1 2004 Previously, we demonstrated that malignant glioma cell lines have increased intracellular pH (pHi) as a result of increased activities of the type I sodium/hydrogen exchanger (NHE1). Sodium 149-155 glucose-6-phosphate isomerase Homo sapiens 94-97 15010500-1 2004 Previously, we demonstrated that malignant glioma cell lines have increased intracellular pH (pHi) as a result of increased activities of the type I sodium/hydrogen exchanger (NHE1). Hydrogen 156-164 glucose-6-phosphate isomerase Homo sapiens 94-97 15010500-8 2004 These findings indicated that amiloride"s cytotoxic effects on glioma cells are independent of its ability to inhibit NHE1 or to reduce intracellular pHi. Amiloride 30-39 glucose-6-phosphate isomerase Homo sapiens 150-153 15103138-1 2004 Glucose-6-phosphate isomerase (PGI; EC 5.3.1.9; also often called by its old nomenclature phosphoglucose isomerase) is an intracellular enzyme that catalyses the reversible conversion of D-glucose 6-phosphate (G6P) to D-fructose 6-phosphate (F6P). Glucose-6-Phosphate 210-213 glucose-6-phosphate isomerase Homo sapiens 31-34 15103138-1 2004 Glucose-6-phosphate isomerase (PGI; EC 5.3.1.9; also often called by its old nomenclature phosphoglucose isomerase) is an intracellular enzyme that catalyses the reversible conversion of D-glucose 6-phosphate (G6P) to D-fructose 6-phosphate (F6P). fructose-6-phosphate 218-240 glucose-6-phosphate isomerase Homo sapiens 31-34 15103138-1 2004 Glucose-6-phosphate isomerase (PGI; EC 5.3.1.9; also often called by its old nomenclature phosphoglucose isomerase) is an intracellular enzyme that catalyses the reversible conversion of D-glucose 6-phosphate (G6P) to D-fructose 6-phosphate (F6P). fructose-6-phosphate 242-245 glucose-6-phosphate isomerase Homo sapiens 31-34 14603531-4 2004 HRT-18 and Caco-2 cells cultured in medium with bicarbonate maintained a pHi of approximately 7.6, which is higher than that of non-neoplastic cells. Bicarbonates 48-59 glucose-6-phosphate isomerase Homo sapiens 73-76 14975458-7 2004 In contrast, the combination of nimesulide and aspirin inhibited PGI-M excretion to a greater extent than aspirin (p = 0.001). nimesulide 32-42 glucose-6-phosphate isomerase Homo sapiens 65-68 14975458-7 2004 In contrast, the combination of nimesulide and aspirin inhibited PGI-M excretion to a greater extent than aspirin (p = 0.001). Aspirin 47-54 glucose-6-phosphate isomerase Homo sapiens 65-68 15128240-1 2004 We demonstrate that sterically bulky N,N"-disubstituted cyclic thiourea-Pd(0) complexes are air- and moisture-stable and highly active catalysts for palladium-catalyzed Heck reaction of aryl iodides and bromides with olefins (TONs up to 500000 for the reaction of PhI and methyl acrylate). n,n"-disubstituted 37-55 glucose-6-phosphate isomerase Homo sapiens 264-267 15128240-1 2004 We demonstrate that sterically bulky N,N"-disubstituted cyclic thiourea-Pd(0) complexes are air- and moisture-stable and highly active catalysts for palladium-catalyzed Heck reaction of aryl iodides and bromides with olefins (TONs up to 500000 for the reaction of PhI and methyl acrylate). cyclic thiourea-pd(0) 56-77 glucose-6-phosphate isomerase Homo sapiens 264-267 15128240-1 2004 We demonstrate that sterically bulky N,N"-disubstituted cyclic thiourea-Pd(0) complexes are air- and moisture-stable and highly active catalysts for palladium-catalyzed Heck reaction of aryl iodides and bromides with olefins (TONs up to 500000 for the reaction of PhI and methyl acrylate). Palladium 149-158 glucose-6-phosphate isomerase Homo sapiens 264-267 15128240-1 2004 We demonstrate that sterically bulky N,N"-disubstituted cyclic thiourea-Pd(0) complexes are air- and moisture-stable and highly active catalysts for palladium-catalyzed Heck reaction of aryl iodides and bromides with olefins (TONs up to 500000 for the reaction of PhI and methyl acrylate). aryl iodides 186-198 glucose-6-phosphate isomerase Homo sapiens 264-267 15128240-1 2004 We demonstrate that sterically bulky N,N"-disubstituted cyclic thiourea-Pd(0) complexes are air- and moisture-stable and highly active catalysts for palladium-catalyzed Heck reaction of aryl iodides and bromides with olefins (TONs up to 500000 for the reaction of PhI and methyl acrylate). Bromides 203-211 glucose-6-phosphate isomerase Homo sapiens 264-267 15128240-1 2004 We demonstrate that sterically bulky N,N"-disubstituted cyclic thiourea-Pd(0) complexes are air- and moisture-stable and highly active catalysts for palladium-catalyzed Heck reaction of aryl iodides and bromides with olefins (TONs up to 500000 for the reaction of PhI and methyl acrylate). Alkenes 217-224 glucose-6-phosphate isomerase Homo sapiens 264-267 15128240-1 2004 We demonstrate that sterically bulky N,N"-disubstituted cyclic thiourea-Pd(0) complexes are air- and moisture-stable and highly active catalysts for palladium-catalyzed Heck reaction of aryl iodides and bromides with olefins (TONs up to 500000 for the reaction of PhI and methyl acrylate). methyl acrylate 272-287 glucose-6-phosphate isomerase Homo sapiens 264-267 15122671-1 2004 The intracellular pH (pHi) of a series of cancer cell lines was determined using the pH-sensitive indicators imidazole (Im) or histidine (His) and diffusion-weighted (DW) proton NMR spectroscopy. imidazole 109-118 glucose-6-phosphate isomerase Homo sapiens 22-25 15122671-1 2004 The intracellular pH (pHi) of a series of cancer cell lines was determined using the pH-sensitive indicators imidazole (Im) or histidine (His) and diffusion-weighted (DW) proton NMR spectroscopy. Histidine 127-136 glucose-6-phosphate isomerase Homo sapiens 22-25 15122671-1 2004 The intracellular pH (pHi) of a series of cancer cell lines was determined using the pH-sensitive indicators imidazole (Im) or histidine (His) and diffusion-weighted (DW) proton NMR spectroscopy. Histidine 138-141 glucose-6-phosphate isomerase Homo sapiens 22-25 15122671-3 2004 Using the chemical shift difference (deltadelta) between the imidazole ring C2-H and C4(5)-H peaks, we were able to measure the pHi independently of chemical shift standardization. deltadelta 37-47 glucose-6-phosphate isomerase Homo sapiens 128-131 15122671-3 2004 Using the chemical shift difference (deltadelta) between the imidazole ring C2-H and C4(5)-H peaks, we were able to measure the pHi independently of chemical shift standardization. imidazole 61-70 glucose-6-phosphate isomerase Homo sapiens 128-131 15122671-5 2004 An inverse correlation was also found between pHi and the intracellular lactate concentration. Lactic Acid 72-79 glucose-6-phosphate isomerase Homo sapiens 46-49 15097861-3 2004 As bicarbonate is a buffer ion, the accurate measurement of intracellular pH (pHi) in duct cells is an important technique for studying the mechanisms of bicarbonate transport. Bicarbonates 3-14 glucose-6-phosphate isomerase Homo sapiens 78-81 15097861-3 2004 As bicarbonate is a buffer ion, the accurate measurement of intracellular pH (pHi) in duct cells is an important technique for studying the mechanisms of bicarbonate transport. Bicarbonates 154-165 glucose-6-phosphate isomerase Homo sapiens 78-81 15097861-4 2004 Commonly, pHi is measured using the fluorescent dye biscarboxyethylcarboxyfluorescein (BCECF). biscarboxyethylcarboxyfluorescein 52-85 glucose-6-phosphate isomerase Homo sapiens 10-13 15097861-4 2004 Commonly, pHi is measured using the fluorescent dye biscarboxyethylcarboxyfluorescein (BCECF). bcecf 87-92 glucose-6-phosphate isomerase Homo sapiens 10-13 15097861-6 2004 Our results indicate that BCECF fluorescence is not only dependent on pHi but also on the total fluorescence intensity of the detected area (which may be influenced by dye loading, dye leakage, and the shutter size on the photomultiplier). bcecf 26-31 glucose-6-phosphate isomerase Homo sapiens 70-73 14603531-5 2004 Cells grown in bicarbonate-free medium with a pH at 6.8 showed a reduction in pHi to approximately 7.0. Bicarbonates 15-26 glucose-6-phosphate isomerase Homo sapiens 46-48 14603531-5 2004 Cells grown in bicarbonate-free medium with a pH at 6.8 showed a reduction in pHi to approximately 7.0. Bicarbonates 15-26 glucose-6-phosphate isomerase Homo sapiens 78-81 14603531-7 2004 When cells were grown in bicarbonate-supplemented medium at pH 6.8, pHi maintained at approximately 7.6 and COX-2 expression was not inhibited. Bicarbonates 25-36 glucose-6-phosphate isomerase Homo sapiens 60-62 14603531-7 2004 When cells were grown in bicarbonate-supplemented medium at pH 6.8, pHi maintained at approximately 7.6 and COX-2 expression was not inhibited. Bicarbonates 25-36 glucose-6-phosphate isomerase Homo sapiens 68-71 14603531-8 2004 Additionally, analysis utilizing protein synthesis inhibitor cycloheximide demonstrated that pHi mediated inhibition of COX-2 mRNA expression requires de novo protein synthesis of regulatory protein(s). Cycloheximide 61-74 glucose-6-phosphate isomerase Homo sapiens 93-96 14707587-1 2004 OBJECTIVE: To determine the effect of gastric feeding on the measurement of gastric intramucosal PCO2 (PiCO2) and its derived gastric intramucosal PCO2-arterial PCO2 difference (PiCO2-PaCO2 difference) and gastric intramucosal pH (pHi) in a group of critically ill children using recirculating gas tonometry. pico2 103-108 glucose-6-phosphate isomerase Homo sapiens 231-234 14715248-1 2004 Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) catalyzes the isomerization between glucose-6-phosphate and fructose-6-phosphate, and is involved in cytokine activity, mitogenesis, differentiation, oncogenesis, and tumor metastasis. Glucose-6-Phosphate 97-116 glucose-6-phosphate isomerase Homo sapiens 0-24 14715248-1 2004 Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) catalyzes the isomerization between glucose-6-phosphate and fructose-6-phosphate, and is involved in cytokine activity, mitogenesis, differentiation, oncogenesis, and tumor metastasis. Glucose-6-Phosphate 97-116 glucose-6-phosphate isomerase Homo sapiens 25-50 14715248-1 2004 Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) catalyzes the isomerization between glucose-6-phosphate and fructose-6-phosphate, and is involved in cytokine activity, mitogenesis, differentiation, oncogenesis, and tumor metastasis. Glucose-6-Phosphate 97-116 glucose-6-phosphate isomerase Homo sapiens 52-55 14715248-1 2004 Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) catalyzes the isomerization between glucose-6-phosphate and fructose-6-phosphate, and is involved in cytokine activity, mitogenesis, differentiation, oncogenesis, and tumor metastasis. fructose-6-phosphate 121-141 glucose-6-phosphate isomerase Homo sapiens 0-24 14715248-1 2004 Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) catalyzes the isomerization between glucose-6-phosphate and fructose-6-phosphate, and is involved in cytokine activity, mitogenesis, differentiation, oncogenesis, and tumor metastasis. fructose-6-phosphate 121-141 glucose-6-phosphate isomerase Homo sapiens 25-50 14715248-1 2004 Phosphoglucose isomerase/autocrine motility factor (PGI/AMF) catalyzes the isomerization between glucose-6-phosphate and fructose-6-phosphate, and is involved in cytokine activity, mitogenesis, differentiation, oncogenesis, and tumor metastasis. fructose-6-phosphate 121-141 glucose-6-phosphate isomerase Homo sapiens 52-55 14715248-3 2004 Inhibition of PGI/AMF by PGI/AMF specific inhibitor 5-phospho-D-arabinonate markedly repressed the cellular migration. 5-phospho-d-arabinonate 52-75 glucose-6-phosphate isomerase Homo sapiens 14-17 14715248-3 2004 Inhibition of PGI/AMF by PGI/AMF specific inhibitor 5-phospho-D-arabinonate markedly repressed the cellular migration. 5-phospho-d-arabinonate 52-75 glucose-6-phosphate isomerase Homo sapiens 25-28 12901768-4 2003 The simultaneous change of Phi and SEE in the case of oxygen covered tungsten have been pointed out and a direct relationship between them can be supposed. Oxygen 54-60 glucose-6-phosphate isomerase Homo sapiens 27-30 14679524-5 2003 For both structures, the canting of the guanine planes on the coordination plane is right-handed (R; canting angle (Phi) of 80.9 degrees and 73.2 degrees, respectively); this indicates that the canting direction is driven by the HT conformation chirality (Delta for both compounds) and not by the chirality of the carrier ligand (different for the two compounds). Guanine 40-47 glucose-6-phosphate isomerase Homo sapiens 116-119 14679524-7 2003 The increase in energy for Phi values below 65 degrees and 55 degrees (for 1 and 2, respectively) is mainly due to the short intramolecular contacts between C(8)H and the cis N-Me groups on the same side of the platinum coordination plane. cis n-me 171-179 glucose-6-phosphate isomerase Homo sapiens 27-30 14679524-7 2003 The increase in energy for Phi values below 65 degrees and 55 degrees (for 1 and 2, respectively) is mainly due to the short intramolecular contacts between C(8)H and the cis N-Me groups on the same side of the platinum coordination plane. Platinum 211-219 glucose-6-phosphate isomerase Homo sapiens 27-30 14586688-1 2003 A simultaneous quantification system of ionic dissociative metabolites was developed using a Fourier transform mid-infrared spectroscopic method by focusing our attention on the enzyme reaction from glucose 6-phosphate to fructose 6-phosphate with phosphoglucose isomerase (PGI). Glucose-6-Phosphate 199-218 glucose-6-phosphate isomerase Homo sapiens 248-272 14586688-1 2003 A simultaneous quantification system of ionic dissociative metabolites was developed using a Fourier transform mid-infrared spectroscopic method by focusing our attention on the enzyme reaction from glucose 6-phosphate to fructose 6-phosphate with phosphoglucose isomerase (PGI). Glucose-6-Phosphate 199-218 glucose-6-phosphate isomerase Homo sapiens 274-277 14586688-1 2003 A simultaneous quantification system of ionic dissociative metabolites was developed using a Fourier transform mid-infrared spectroscopic method by focusing our attention on the enzyme reaction from glucose 6-phosphate to fructose 6-phosphate with phosphoglucose isomerase (PGI). fructose-6-phosphate 222-242 glucose-6-phosphate isomerase Homo sapiens 248-272 14586688-1 2003 A simultaneous quantification system of ionic dissociative metabolites was developed using a Fourier transform mid-infrared spectroscopic method by focusing our attention on the enzyme reaction from glucose 6-phosphate to fructose 6-phosphate with phosphoglucose isomerase (PGI). fructose-6-phosphate 222-242 glucose-6-phosphate isomerase Homo sapiens 274-277 12888574-7 2003 PGI is enzymatically active as a dimer and we show here by non-denaturing gel electrophoresis as well as by glutaraldehyde cross-linking that it exists at neutral pH in a tetrameric form. Glutaral 108-122 glucose-6-phosphate isomerase Homo sapiens 0-3 12890576-7 2003 These data indicate that adrenaline induces an increase of pHi and Na(+) uptake of human erythrocytes through stimulation of NHE 1 activity. Epinephrine 25-35 glucose-6-phosphate isomerase Homo sapiens 59-62 14976408-4 2004 The pHi was measured using the pH-sensitive dye 2 , 7 -BIS (2-carboxyethyl-5-(6)-carboxyfluorescein. 2 , 7 -bis (2-carboxyethyl-5-(6)-carboxyfluorescein 48-99 glucose-6-phosphate isomerase Homo sapiens 4-7 14976408-7 2004 In Caco-2 cells, quercetin and flavone increased early and late apoptosis parameters associated with a concomitant decline in pHi. Quercetin 17-26 glucose-6-phosphate isomerase Homo sapiens 126-129 14976408-7 2004 In Caco-2 cells, quercetin and flavone increased early and late apoptosis parameters associated with a concomitant decline in pHi. flavone 31-38 glucose-6-phosphate isomerase Homo sapiens 126-129 14976408-10 2004 Here too, imidazole prevented the pHi decline but failed to affect apoptosis execution. imidazole 10-19 glucose-6-phosphate isomerase Homo sapiens 34-37 14645139-0 2003 Docosahexaenoic acid and other fatty acids induce a decrease in pHi in Jurkat T-cells. Docosahexaenoic Acids 0-20 glucose-6-phosphate isomerase Homo sapiens 64-67 14645139-0 2003 Docosahexaenoic acid and other fatty acids induce a decrease in pHi in Jurkat T-cells. Fatty Acids 31-42 glucose-6-phosphate isomerase Homo sapiens 64-67 14645139-2 2003 Docosahexaenoic acid (DHA) induced rapid (t1/2=33 s) and dose-dependent decreases in pHi in BCECF-loaded human (Jurkat) T-cells. Docosahexaenoic Acids 0-20 glucose-6-phosphate isomerase Homo sapiens 85-88 14645139-2 2003 Docosahexaenoic acid (DHA) induced rapid (t1/2=33 s) and dose-dependent decreases in pHi in BCECF-loaded human (Jurkat) T-cells. Docosahexaenoic Acids 22-25 glucose-6-phosphate isomerase Homo sapiens 85-88 14645139-3 2003 Addition of 5-(N,N-dimethyl)-amiloride, an inhibitor of Na+/H+ exchanger, prolonged DHA-induced acidification as a function of time, indicating that the exchanger is implicated in pHi recovery. 5-dimethylamiloride 12-38 glucose-6-phosphate isomerase Homo sapiens 180-183 14645139-3 2003 Addition of 5-(N,N-dimethyl)-amiloride, an inhibitor of Na+/H+ exchanger, prolonged DHA-induced acidification as a function of time, indicating that the exchanger is implicated in pHi recovery. Docosahexaenoic Acids 84-87 glucose-6-phosphate isomerase Homo sapiens 180-183 14645139-5 2003 Other fatty acids like oleic acid, arachidonic acid, eicosapentaenoic acid, but not palmitic acid, also induced a fall in pHi in these cells. Fatty Acids 6-17 glucose-6-phosphate isomerase Homo sapiens 122-125 14645139-5 2003 Other fatty acids like oleic acid, arachidonic acid, eicosapentaenoic acid, but not palmitic acid, also induced a fall in pHi in these cells. Oleic Acid 23-33 glucose-6-phosphate isomerase Homo sapiens 122-125 14645139-5 2003 Other fatty acids like oleic acid, arachidonic acid, eicosapentaenoic acid, but not palmitic acid, also induced a fall in pHi in these cells. Arachidonic Acid 35-51 glucose-6-phosphate isomerase Homo sapiens 122-125 14645139-5 2003 Other fatty acids like oleic acid, arachidonic acid, eicosapentaenoic acid, but not palmitic acid, also induced a fall in pHi in these cells. Eicosapentaenoic Acid 53-74 glucose-6-phosphate isomerase Homo sapiens 122-125 14645139-11 2003 Furthermore, addition of CaCl2 into 0% Ca2+ medium curtailed DHA-evoked rapid pHi recovery. Calcium Chloride 25-30 glucose-6-phosphate isomerase Homo sapiens 78-81 14645139-11 2003 Furthermore, addition of CaCl2 into 0% Ca2+ medium curtailed DHA-evoked rapid pHi recovery. Docosahexaenoic Acids 61-64 glucose-6-phosphate isomerase Homo sapiens 78-81 14703502-9 2003 The recombinant gpI was expressed in insect cells Sf 9, post-infected with recombinant baculovirus and identified by SDS-PAGE and western blotting, with its product in cell culture reaching the peak in 72 hours and with a molecular mass of 58 kd and 70 kd, the same as theoretical values. Sodium Dodecyl Sulfate 117-120 glucose-6-phosphate isomerase Homo sapiens 16-19 12890576-2 2003 Adrenaline increased both intracellular pH (pHi) and Na(+) influx in erythrocyte suspensions. Epinephrine 0-10 glucose-6-phosphate isomerase Homo sapiens 44-47 12682061-2 2003 The 5-HT2A receptor possesses a canonical Type I PDZ-binding domain (X-Ser/Thr-X-Phi) at the carboxyl terminus and has been predicted, but never demonstrated, to interact with PDZ domain-containing proteins. Serine 71-74 glucose-6-phosphate isomerase Homo sapiens 81-84 12890370-5 2003 RESULTS: At the end of surgery,the Ps-a CO(2) gap in the HES group (8.7 +/- 1.6 mmHg) was significantly lower than that of the LRS group (18.74 +/- 4.4 mmHg, P < 0.01), while the pHi (7.30 +/- 0.05 mmHg) in the HES group was significantly higher than that of the LRS group (7.21 +/- 0.07 mmHg, P < 0.01). Hydroxyethyl Starch Derivatives 57-60 glucose-6-phosphate isomerase Homo sapiens 182-185 12682061-2 2003 The 5-HT2A receptor possesses a canonical Type I PDZ-binding domain (X-Ser/Thr-X-Phi) at the carboxyl terminus and has been predicted, but never demonstrated, to interact with PDZ domain-containing proteins. Threonine 75-78 glucose-6-phosphate isomerase Homo sapiens 81-84 12777791-1 2003 Phosphoglucose isomerase (PGI) is a workhorse enzyme of carbohydrate metabolism that interconverts glucose 6-phosphate and fructose 6-phosphate. Glucose-6-Phosphate 99-118 glucose-6-phosphate isomerase Homo sapiens 0-24 12777791-1 2003 Phosphoglucose isomerase (PGI) is a workhorse enzyme of carbohydrate metabolism that interconverts glucose 6-phosphate and fructose 6-phosphate. Glucose-6-Phosphate 99-118 glucose-6-phosphate isomerase Homo sapiens 26-29 12777791-1 2003 Phosphoglucose isomerase (PGI) is a workhorse enzyme of carbohydrate metabolism that interconverts glucose 6-phosphate and fructose 6-phosphate. Carbohydrates 56-68 glucose-6-phosphate isomerase Homo sapiens 0-24 12777791-1 2003 Phosphoglucose isomerase (PGI) is a workhorse enzyme of carbohydrate metabolism that interconverts glucose 6-phosphate and fructose 6-phosphate. fructose-6-phosphate 123-143 glucose-6-phosphate isomerase Homo sapiens 0-24 12777791-1 2003 Phosphoglucose isomerase (PGI) is a workhorse enzyme of carbohydrate metabolism that interconverts glucose 6-phosphate and fructose 6-phosphate. Carbohydrates 56-68 glucose-6-phosphate isomerase Homo sapiens 26-29 12777791-1 2003 Phosphoglucose isomerase (PGI) is a workhorse enzyme of carbohydrate metabolism that interconverts glucose 6-phosphate and fructose 6-phosphate. fructose-6-phosphate 123-143 glucose-6-phosphate isomerase Homo sapiens 26-29 12777791-3 2003 A crystal structure of human PGI bound with 5-phosphoarabinonate, a strong inhibitor that mimics the cis-enediol(ate) intermediate of the reaction, has been determined at 2.5 A resolution. arabinonate-5-phosphate 44-64 glucose-6-phosphate isomerase Homo sapiens 29-32 12777791-3 2003 A crystal structure of human PGI bound with 5-phosphoarabinonate, a strong inhibitor that mimics the cis-enediol(ate) intermediate of the reaction, has been determined at 2.5 A resolution. cis-enediol 101-112 glucose-6-phosphate isomerase Homo sapiens 29-32 12777791-3 2003 A crystal structure of human PGI bound with 5-phosphoarabinonate, a strong inhibitor that mimics the cis-enediol(ate) intermediate of the reaction, has been determined at 2.5 A resolution. ate 61-64 glucose-6-phosphate isomerase Homo sapiens 29-32 12659967-5 2003 By contrast, adding to the medium ammonium or imidazole, which increased pHi, had no effect on GC uptake. Ammonium Compounds 34-42 glucose-6-phosphate isomerase Homo sapiens 73-76 12846988-4 2003 DA also acts on receptors in GPe, GPi, SNr, and STN. Dopamine 0-2 glucose-6-phosphate isomerase Homo sapiens 34-37 12846988-5 2003 Electrophysiologic and other studies in primates rendered parkinsonian by treatment with the dopaminergic neurotoxin MPTP have demonstrated a reduction of neuronal activity of GPe and an increase of neuronal discharge in STN, GPi. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 117-121 glucose-6-phosphate isomerase Homo sapiens 226-229 12737586-1 2003 A GPI-anchored dipeptide of sperm CD52 antigen was prepared through a convergent synthesis. Dipeptides 15-24 glucose-6-phosphate isomerase Homo sapiens 2-5 12737586-2 2003 First, the dipeptide with its C-terminus free and the GPI with its nonreducing end phosphoethanolamine bearing a free amino group were synthesized separately. phosphorylethanolamine 83-102 glucose-6-phosphate isomerase Homo sapiens 54-57 12850843-8 2003 The thrombin-evoked increase in pHi was markedly inhibited in the presence of kinetin (70 and 150 microM). Kinetin 78-85 glucose-6-phosphate isomerase Homo sapiens 32-35 12659967-5 2003 By contrast, adding to the medium ammonium or imidazole, which increased pHi, had no effect on GC uptake. imidazole 46-55 glucose-6-phosphate isomerase Homo sapiens 73-76 12659967-8 2003 However, GC uptake was inhibited at pHo 7.40 and 7.80 when pHo>pHi. Glycocholic Acid 9-11 glucose-6-phosphate isomerase Homo sapiens 66-69 12562898-3 2003 In normal, but not CF, epithelia, replacing mucosal Cl- with gluconate caused intracellular pH (pHi) to increase, and the initial rates (Delta pH min-1) of this increase were modestly augmented (approximately 26 %) when normal cells were pretreated with forskolin (10 microM). gluconic acid 61-70 glucose-6-phosphate isomerase Homo sapiens 96-99 12660225-2 2003 The aim of the present work was to study the effect of ANP on the intracellular pH (pHi) of human monocytes and macrophages and to investigate whether pHi changes could play a role on phospholipase activities and reactive oxygen species (ROS) production. Reactive Oxygen Species 213-236 glucose-6-phosphate isomerase Homo sapiens 151-154 12660225-2 2003 The aim of the present work was to study the effect of ANP on the intracellular pH (pHi) of human monocytes and macrophages and to investigate whether pHi changes could play a role on phospholipase activities and reactive oxygen species (ROS) production. Reactive Oxygen Species 238-241 glucose-6-phosphate isomerase Homo sapiens 151-154 12660225-4 2003 A significant decrease of pHi was observed in ANP-treated macrophages with respect to untreated cells; this effect was paralleled by enhanced phospholipase activity and ROS production. Reactive Oxygen Species 169-172 glucose-6-phosphate isomerase Homo sapiens 26-29 12660225-7 2003 Treatment of macrophages or THP-1 monocytes with 5-(N-ethyl-N-isopropyl)amiloride, a specific Na(+)/H(+) antiport inhibitor, decreases pHi in macrophages and monocytes. ethylisopropylamiloride 49-81 glucose-6-phosphate isomerase Homo sapiens 135-138 12700282-1 2003 This study tests the hypothesis that lowering intracellular pH (pHi) in melanoma cells grown at low extracellular pH (pHe) selectively abrogates 42 degrees C-induced heat shock protein (HSP) expression and reduces survival. Phenylalanine 118-121 glucose-6-phosphate isomerase Homo sapiens 64-67 12700282-6 2003 The effect of extracellular acidification combined with CNCn on pHi was measured in cells grown at pHe 6.7 and pHe 7.3. alpha-cyano-4-hydroxycinnamate 56-60 glucose-6-phosphate isomerase Homo sapiens 64-67 12700282-6 2003 The effect of extracellular acidification combined with CNCn on pHi was measured in cells grown at pHe 6.7 and pHe 7.3. Phenylalanine 111-114 glucose-6-phosphate isomerase Homo sapiens 64-67 12700282-8 2003 When cells were grown at pHe 6.7 and incubated with CNCn at pHe 6.5, the pHi decreased from 6.9 to below 6.5, and the 42 degrees C induction of HSP70 and HSP27 was blocked. alpha-cyano-4-hydroxycinnamate 52-56 glucose-6-phosphate isomerase Homo sapiens 73-76 12537996-7 2003 In contrast, D-Dmp(1)- or Phe(1)-substitution of Tyr(1) resulted in a significant decrease in mu-receptor affinity and GPI potency. d-dmp 13-18 glucose-6-phosphate isomerase Homo sapiens 94-122 12584249-1 2003 Internalization of autocrine motility factor (AMF) into the endoplasmic reticulum is sensitive to the cholesterol-extracting reagent methyl-beta-cyclodextrin, inhibited by the dynamin-1 K44A mutant and negatively regulated by caveolin-1. Cholesterol 102-113 glucose-6-phosphate isomerase Homo sapiens 19-44 12584249-1 2003 Internalization of autocrine motility factor (AMF) into the endoplasmic reticulum is sensitive to the cholesterol-extracting reagent methyl-beta-cyclodextrin, inhibited by the dynamin-1 K44A mutant and negatively regulated by caveolin-1. Cholesterol 102-113 glucose-6-phosphate isomerase Homo sapiens 46-49 12584249-1 2003 Internalization of autocrine motility factor (AMF) into the endoplasmic reticulum is sensitive to the cholesterol-extracting reagent methyl-beta-cyclodextrin, inhibited by the dynamin-1 K44A mutant and negatively regulated by caveolin-1. methyl-beta-cyclodextrin 133-157 glucose-6-phosphate isomerase Homo sapiens 19-44 12584249-1 2003 Internalization of autocrine motility factor (AMF) into the endoplasmic reticulum is sensitive to the cholesterol-extracting reagent methyl-beta-cyclodextrin, inhibited by the dynamin-1 K44A mutant and negatively regulated by caveolin-1. methyl-beta-cyclodextrin 133-157 glucose-6-phosphate isomerase Homo sapiens 46-49 12537996-7 2003 In contrast, D-Dmp(1)- or Phe(1)-substitution of Tyr(1) resulted in a significant decrease in mu-receptor affinity and GPI potency. Phenylalanine 26-29 glucose-6-phosphate isomerase Homo sapiens 94-122 12537996-7 2003 In contrast, D-Dmp(1)- or Phe(1)-substitution of Tyr(1) resulted in a significant decrease in mu-receptor affinity and GPI potency. Tyrosine 49-52 glucose-6-phosphate isomerase Homo sapiens 94-122 12496272-3 2003 The primary structure of the newly identified HDL-binding protein resembles GPI-anchored proteins consisting of an N-terminal signal sequence, an acidic region with a cluster of aspartate and glutamate residues, an Ly-6 motif highly conserved among the lymphocyte antigen family, and a C-terminal hydrophobic region. Aspartic Acid 178-187 glucose-6-phosphate isomerase Homo sapiens 76-79 12576545-3 2003 Analysis of substrate specificity shows that GDE1/MIR16 selectively hydrolyzes GPI over glycerophosphocholine. Glycerylphosphorylcholine 88-109 glucose-6-phosphate isomerase Homo sapiens 79-82 12576545-6 2003 Our results suggest that by serving as a PDE for GPI with its activity regulated by G protein signaling, GDE1/MIR16 provides a link between phosphoinositide metabolism and G protein signal transduction. Phosphatidylinositols 140-156 glucose-6-phosphate isomerase Homo sapiens 49-52 12603086-4 2003 The mean intracellular pH (pHi) in patients with COPD was similar to that of age-matched controls, but decreased in the patients with COPD by a mean pHi of 0.02 (p = 0.04), following supplemental oxygen. Oxygen 196-202 glucose-6-phosphate isomerase Homo sapiens 27-30 12603086-6 2003 The broadband component of the MR spectrum increased in all the patients with COPD (p = 0.01), suggesting altered phospholipid membrane fluidity in the brain associated with the change in pHi following oxygen administration. Phospholipids 114-126 glucose-6-phosphate isomerase Homo sapiens 188-191 12603086-6 2003 The broadband component of the MR spectrum increased in all the patients with COPD (p = 0.01), suggesting altered phospholipid membrane fluidity in the brain associated with the change in pHi following oxygen administration. Oxygen 202-208 glucose-6-phosphate isomerase Homo sapiens 188-191 12573240-1 2003 The second enzyme in the glycolytic pathway, phosphoglucose isomerase (PGI), catalyses an intracellular aldose-ketose isomerization. Ketoses 111-117 glucose-6-phosphate isomerase Homo sapiens 45-69 12573240-1 2003 The second enzyme in the glycolytic pathway, phosphoglucose isomerase (PGI), catalyses an intracellular aldose-ketose isomerization. Ketoses 111-117 glucose-6-phosphate isomerase Homo sapiens 71-74 12496272-3 2003 The primary structure of the newly identified HDL-binding protein resembles GPI-anchored proteins consisting of an N-terminal signal sequence, an acidic region with a cluster of aspartate and glutamate residues, an Ly-6 motif highly conserved among the lymphocyte antigen family, and a C-terminal hydrophobic region. Glutamic Acid 192-201 glucose-6-phosphate isomerase Homo sapiens 76-79 12558316-7 2003 The lowest value of pHi during CPB was significantly related to blood urea nitrogen (r = -0.75, p < 0.05), serum creatinine (r = -0.78, p < 0.05), creatinine clearance (r = 0.68, p < 0.05) on postoperative day 1, and blood urea nitrogen (r = -0.84, p < 0.01) on day 3. Urea 70-74 glucose-6-phosphate isomerase Homo sapiens 20-23 12812678-5 2003 RESULTS: At the end of surgery, the Ps-aCO(2) gap was 8.7 mm Hg +/- 1.6 mm Hg in the HES group, significantly lower than that in the LRS group (18.74 mm Hg +/- 4.4 mm Hg, P < 0.01), while the pHi in the HES group was 7.30 +/- 0.05, significantly higher than that in the LRS group (7.21 +/- 0.07, P < 0.01). Hydroxyethyl Starch Derivatives 85-88 glucose-6-phosphate isomerase Homo sapiens 195-198 12701204-5 2003 We found that FDG-6-phosphate was further isomerized to 18F-FDM-6-phosphate by phosphoglucose isomerase (PGI) in vitro. 2-fluoro-2-deoxyglucose-6-phosphate 14-29 glucose-6-phosphate isomerase Homo sapiens 79-103 12701204-5 2003 We found that FDG-6-phosphate was further isomerized to 18F-FDM-6-phosphate by phosphoglucose isomerase (PGI) in vitro. 2-fluoro-2-deoxyglucose-6-phosphate 14-29 glucose-6-phosphate isomerase Homo sapiens 105-108 12701204-5 2003 We found that FDG-6-phosphate was further isomerized to 18F-FDM-6-phosphate by phosphoglucose isomerase (PGI) in vitro. 18f-fdm-6-phosphate 56-75 glucose-6-phosphate isomerase Homo sapiens 79-103 12701204-5 2003 We found that FDG-6-phosphate was further isomerized to 18F-FDM-6-phosphate by phosphoglucose isomerase (PGI) in vitro. 18f-fdm-6-phosphate 56-75 glucose-6-phosphate isomerase Homo sapiens 105-108 12701204-6 2003 About 27% 18F-FDM-6-phosphate was generated at the reaction with 70 U PGI for 90 min. 18f-fdm-6-phosphate 10-29 glucose-6-phosphate isomerase Homo sapiens 70-73 12558316-7 2003 The lowest value of pHi during CPB was significantly related to blood urea nitrogen (r = -0.75, p < 0.05), serum creatinine (r = -0.78, p < 0.05), creatinine clearance (r = 0.68, p < 0.05) on postoperative day 1, and blood urea nitrogen (r = -0.84, p < 0.01) on day 3. Nitrogen 75-83 glucose-6-phosphate isomerase Homo sapiens 20-23 12952503-1 2003 Iometopane [(123)I beta-CIT, GPI 200, RTI 55], a tropane derivative labelled with iodine-123, is a dopamine imaging agent that was under development with Guilford Pharmaceuticals (as Dopascan Injection) for the early diagnosis of Parkinson"s disease. 2beta-carbomethoxy-3beta-(4-iodophenyl)tropane 0-10 glucose-6-phosphate isomerase Homo sapiens 29-32 12558316-7 2003 The lowest value of pHi during CPB was significantly related to blood urea nitrogen (r = -0.75, p < 0.05), serum creatinine (r = -0.78, p < 0.05), creatinine clearance (r = 0.68, p < 0.05) on postoperative day 1, and blood urea nitrogen (r = -0.84, p < 0.01) on day 3. Creatinine 116-126 glucose-6-phosphate isomerase Homo sapiens 20-23 12558316-7 2003 The lowest value of pHi during CPB was significantly related to blood urea nitrogen (r = -0.75, p < 0.05), serum creatinine (r = -0.78, p < 0.05), creatinine clearance (r = 0.68, p < 0.05) on postoperative day 1, and blood urea nitrogen (r = -0.84, p < 0.01) on day 3. Creatinine 153-163 glucose-6-phosphate isomerase Homo sapiens 20-23 12558316-7 2003 The lowest value of pHi during CPB was significantly related to blood urea nitrogen (r = -0.75, p < 0.05), serum creatinine (r = -0.78, p < 0.05), creatinine clearance (r = 0.68, p < 0.05) on postoperative day 1, and blood urea nitrogen (r = -0.84, p < 0.01) on day 3. Urea 232-236 glucose-6-phosphate isomerase Homo sapiens 20-23 12558316-7 2003 The lowest value of pHi during CPB was significantly related to blood urea nitrogen (r = -0.75, p < 0.05), serum creatinine (r = -0.78, p < 0.05), creatinine clearance (r = 0.68, p < 0.05) on postoperative day 1, and blood urea nitrogen (r = -0.84, p < 0.01) on day 3. Nitrogen 237-245 glucose-6-phosphate isomerase Homo sapiens 20-23 12904124-6 2003 Despite these risks, the benefits of GPI therapy in addition to conventional treatment, such as aspirin and heparin, should be considered for these high-risk patients. Aspirin 96-103 glucose-6-phosphate isomerase Homo sapiens 37-40 12904124-6 2003 Despite these risks, the benefits of GPI therapy in addition to conventional treatment, such as aspirin and heparin, should be considered for these high-risk patients. Heparin 108-115 glucose-6-phosphate isomerase Homo sapiens 37-40 12370494-5 2002 When pHi was neutralized by changing from imidazole-containing medium to fresh medium, oligonucleosomal DNA fragmentation and increased caspase-3 activity was observed in the imidazole-treated HL-60 cells. imidazole 42-51 glucose-6-phosphate isomerase Homo sapiens 5-8 12675272-10 2003 Similarly, pHi recovery from NH4Cl prepulse acid-loads (pHo 7.4) was sensitive to both EIPA and bafilomycin A1. bafilomycin A1 96-110 glucose-6-phosphate isomerase Homo sapiens 11-14 12675272-11 2003 During this recovery process, Na+/H+ exchange (EIPA-sensitive component of apparent H+ efflux) was the predominant mechanism for H+ extrusion at acid-loaded pHi values < 7.0. ethylisopropylamiloride 47-51 glucose-6-phosphate isomerase Homo sapiens 157-160 12675272-14 2003 The H+-pump and NHE interacted to set baseline pHi and for pHi recovery following cytosolic acid-loading of the monocytes. cytosolic acid 82-96 glucose-6-phosphate isomerase Homo sapiens 59-62 12675272-3 2003 The fluorescent probe 2",7"-biscarboxyethyl-5,6-carboxyfluorescein was used to monitor baseline pHi and the kinetics of pHi recovery from cytosolic acid-loads (NH4Cl prepulse). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 22-66 glucose-6-phosphate isomerase Homo sapiens 96-99 12675272-3 2003 The fluorescent probe 2",7"-biscarboxyethyl-5,6-carboxyfluorescein was used to monitor baseline pHi and the kinetics of pHi recovery from cytosolic acid-loads (NH4Cl prepulse). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 22-66 glucose-6-phosphate isomerase Homo sapiens 120-123 12675272-3 2003 The fluorescent probe 2",7"-biscarboxyethyl-5,6-carboxyfluorescein was used to monitor baseline pHi and the kinetics of pHi recovery from cytosolic acid-loads (NH4Cl prepulse). Ammonium Chloride 160-165 glucose-6-phosphate isomerase Homo sapiens 120-123 12675272-6 2003 EIPA had no effect on baseline pHi at pHo 7.4, but caused a sustained decrement in pHi at pHo 6.0-7.0. ethylisopropylamiloride 0-4 glucose-6-phosphate isomerase Homo sapiens 83-86 12675272-7 2003 Bafilomycin A1 had biphasic effects on baseline pHi at pHo 6.5-7.4; pHi declined approximately 0.1 units over the course of several minutes and then recovered. bafilomycin 0-11 glucose-6-phosphate isomerase Homo sapiens 48-51 12675272-10 2003 Similarly, pHi recovery from NH4Cl prepulse acid-loads (pHo 7.4) was sensitive to both EIPA and bafilomycin A1. Ammonium Chloride 29-34 glucose-6-phosphate isomerase Homo sapiens 11-14 12675272-10 2003 Similarly, pHi recovery from NH4Cl prepulse acid-loads (pHo 7.4) was sensitive to both EIPA and bafilomycin A1. ethylisopropylamiloride 87-91 glucose-6-phosphate isomerase Homo sapiens 11-14 12447926-5 2002 pH(i) was calculated using phosphorus-31 MR spectroscopy and lactate/creatine was measured using proton MRS. Phosphorus 27-37 glucose-6-phosphate isomerase Homo sapiens 0-5 12370494-5 2002 When pHi was neutralized by changing from imidazole-containing medium to fresh medium, oligonucleosomal DNA fragmentation and increased caspase-3 activity was observed in the imidazole-treated HL-60 cells. imidazole 175-184 glucose-6-phosphate isomerase Homo sapiens 5-8 12370494-7 2002 These results indicate that imidazole induces caspase-dependent cell death, and suggest that maintaining pHi in the neutral range is essential for the induction of oligonucleosomal DNA fragmentation in the execution phase of apoptosis. imidazole 28-37 glucose-6-phosphate isomerase Homo sapiens 105-108 12213900-0 2002 Human fallopian tubes express prostacyclin (PGI) synthase and cyclooxygenases and synthesize abundant PGI. Epoprostenol 30-42 glucose-6-phosphate isomerase Homo sapiens 44-47 12690673-1 2002 The effects of deep brain stimulation (DBS) of the subthalamic nucleus (STN) or the internal pallidum (GPi) on the parkinsonian triad and on levodopa-induced dyskinesias are very similar. Levodopa 141-149 glucose-6-phosphate isomerase Homo sapiens 103-106 12817715-2 2002 However, the effect of H2O2 on pHi has not been well characterized in the human atrial myocardium. Hydrogen Peroxide 23-27 glucose-6-phosphate isomerase Homo sapiens 31-34 12817715-6 2002 By continuously monitoring pHi changes in human atrial myocardium, we have found, for the first time, that (a) H2O2 (30 microM-3 mM) induced a significant dose-dependent intracellular acidosis, (b) N-MPG caused a significant block on the intracellular acidosis induced by 3 mM H2O2, whereas L-methionine did not, and (c) Hoe 694, a specific Na+/H+ exchanger (NHE) inhibitor, caused a similar extents like that induced by 3 mM H2O2. Hydrogen Peroxide 111-115 glucose-6-phosphate isomerase Homo sapiens 27-30 12817715-8 2002 The possible underlying mechanism for H2O2-induced acidosis is likely due to its inhibition on the activity of NHE and other acid extruders, as the pHi changes after H2O2 exposure could be detected even though the activity of NHE was completely blocked by 30 mM Hoe 694. Hydrogen Peroxide 38-42 glucose-6-phosphate isomerase Homo sapiens 148-151 12817715-8 2002 The possible underlying mechanism for H2O2-induced acidosis is likely due to its inhibition on the activity of NHE and other acid extruders, as the pHi changes after H2O2 exposure could be detected even though the activity of NHE was completely blocked by 30 mM Hoe 694. Hydrogen Peroxide 166-170 glucose-6-phosphate isomerase Homo sapiens 148-151 12374700-7 2002 Treatment of breast cancer cells with the combination of HCT and specific AMF inhibitors, erythrose 4-phosphate or D-mannose 6-phosphate, resulted in an additive inhibitory effect on both the growth rate and invasiveness of cells as compared with treatment with each agent alone. erythrose 4-phosphate 90-111 glucose-6-phosphate isomerase Homo sapiens 74-77 12374700-7 2002 Treatment of breast cancer cells with the combination of HCT and specific AMF inhibitors, erythrose 4-phosphate or D-mannose 6-phosphate, resulted in an additive inhibitory effect on both the growth rate and invasiveness of cells as compared with treatment with each agent alone. mannose-6-phosphate 115-136 glucose-6-phosphate isomerase Homo sapiens 74-77 12213900-6 2002 We found that 6 keto-PGF(1alpha), a stable metabolite of prostacyclin (PGI), and PGE(2) constituted 56% +/- 10% and 35% +/- 10% (mean +/- SEM, four samples), respectively, of total eicosanoids synthesized. 6 keto-pgf 14-24 glucose-6-phosphate isomerase Homo sapiens 71-74 12213900-12 2002 Iloprost, a PGI analog, inhibited the activities of circular and longitudinal muscles of the fallopian tube. Iloprost 0-8 glucose-6-phosphate isomerase Homo sapiens 12-15 12113831-1 2002 An inner core of the GPI anchor of sperm CD52 antigens was synthesized by a highly convergent process using specially modified inositol, glucosamine and phospholipid as key building blocks. Inositol 127-135 glucose-6-phosphate isomerase Homo sapiens 21-24 12133590-5 2002 Astrocytic pH(i) was monitored for 60 min after transferring the cells to HEPES or bicarbonate-buffered saline. bicarbonate-buffered saline 83-110 glucose-6-phosphate isomerase Homo sapiens 11-16 12133590-12 2002 A close association of tyrosine phosphorylation with stellation and pH(i) was observed. Tyrosine 23-31 glucose-6-phosphate isomerase Homo sapiens 68-73 12113831-1 2002 An inner core of the GPI anchor of sperm CD52 antigens was synthesized by a highly convergent process using specially modified inositol, glucosamine and phospholipid as key building blocks. Glucosamine 137-148 glucose-6-phosphate isomerase Homo sapiens 21-24 12113831-1 2002 An inner core of the GPI anchor of sperm CD52 antigens was synthesized by a highly convergent process using specially modified inositol, glucosamine and phospholipid as key building blocks. Phospholipids 153-165 glucose-6-phosphate isomerase Homo sapiens 21-24 12113831-2 2002 This paper also presents a new and efficient procedure to prepare 1,2,6-differentiated derivatives of inositol for GPI syntheses. Inositol 102-110 glucose-6-phosphate isomerase Homo sapiens 115-118 12082541-1 2002 We report the genomic organization of a novel human gene mapped to chromosome 6p21, encoding a putative glycosylphosphatidylinositol (GPI) anchored protein containing a MAM (meprin, A5 antigen, protein tyrosine phosphatase mu) domain, that we have termed as GPIM (GPI and MAM) protein. Glycosylphosphatidylinositols 104-132 glucose-6-phosphate isomerase Homo sapiens 134-137 12081485-4 2002 The GPI-anchored ectoenzyme placental alkaline phosphatase (PLAP) was purified from a plasma membrane extract of human placental microsomes without the use of butanol. Butanols 159-166 glucose-6-phosphate isomerase Homo sapiens 4-7 12479222-2 2002 In general, three families of transporters are capable of removing metabolic protons, but the specific transporters responsible for the maintenance of pHi at low pHe in melanomas have not been identified. Phenylalanine 162-165 glucose-6-phosphate isomerase Homo sapiens 151-154 12027906-2 2002 The aim of the study was to elucidate the mode of action of sulthiame with respect to possible changes of intracellular pH (pHi) that might develop along with sulthiame"s anticonvulsant properties. sulthiame 60-69 glucose-6-phosphate isomerase Homo sapiens 124-127 12027853-1 2002 BACKGROUND: This study was prompted by concern that administration of bicarbonate for correction of lactate acidosis aggravates a low intracellular pH (pHi). Bicarbonates 70-81 glucose-6-phosphate isomerase Homo sapiens 152-155 12027853-9 2002 In the last min of rhythmic handgrip the decrease in pHi was attenuated by the administration of bicarbonate (6.60 +/- 0.11 vs. 6.40 +/- 0.12; P<0.05). Bicarbonates 97-108 glucose-6-phosphate isomerase Homo sapiens 53-56 12054796-3 2002 The crystal structures of the inhibitor-free open form and the inhibitor (erythrose 4-phosphate, E4P, a strong inhibitor of AMF"s cytokine activity)-bound closed form of human AMF have been determined at 1.9 A and 2.4 A resolution, respectively. erythrose 4-phosphate 74-95 glucose-6-phosphate isomerase Homo sapiens 124-127 12054796-3 2002 The crystal structures of the inhibitor-free open form and the inhibitor (erythrose 4-phosphate, E4P, a strong inhibitor of AMF"s cytokine activity)-bound closed form of human AMF have been determined at 1.9 A and 2.4 A resolution, respectively. erythrose 4-phosphate 74-95 glucose-6-phosphate isomerase Homo sapiens 176-179 12054796-3 2002 The crystal structures of the inhibitor-free open form and the inhibitor (erythrose 4-phosphate, E4P, a strong inhibitor of AMF"s cytokine activity)-bound closed form of human AMF have been determined at 1.9 A and 2.4 A resolution, respectively. erythrose 4-phosphate 97-100 glucose-6-phosphate isomerase Homo sapiens 124-127 12054796-3 2002 The crystal structures of the inhibitor-free open form and the inhibitor (erythrose 4-phosphate, E4P, a strong inhibitor of AMF"s cytokine activity)-bound closed form of human AMF have been determined at 1.9 A and 2.4 A resolution, respectively. erythrose 4-phosphate 97-100 glucose-6-phosphate isomerase Homo sapiens 176-179 12054796-5 2002 The E4P-bound structure shows that the location of the inhibitor (of cytokine activity) binding site of human AMF is very similar to those of the inhibitor (of enzymatic activity) binding sites of PHIs. erythrose 4-phosphate 4-7 glucose-6-phosphate isomerase Homo sapiens 110-113 12054796-6 2002 The present study shows clearly that there is structural overlap of the regions responsible for the enzymatic and cytokine functions of AMF and PHI and suggests two scenarios for the inhibition mechanism of cytokine activity of AMF by the carbohydrate phosphate group. carbohydrate phosphate 239-261 glucose-6-phosphate isomerase Homo sapiens 136-139 12054796-6 2002 The present study shows clearly that there is structural overlap of the regions responsible for the enzymatic and cytokine functions of AMF and PHI and suggests two scenarios for the inhibition mechanism of cytokine activity of AMF by the carbohydrate phosphate group. carbohydrate phosphate 239-261 glucose-6-phosphate isomerase Homo sapiens 144-147 12054796-6 2002 The present study shows clearly that there is structural overlap of the regions responsible for the enzymatic and cytokine functions of AMF and PHI and suggests two scenarios for the inhibition mechanism of cytokine activity of AMF by the carbohydrate phosphate group. carbohydrate phosphate 239-261 glucose-6-phosphate isomerase Homo sapiens 228-231 12054796-7 2002 One likely scenario is that the compound could compete for AMF binding with the carbohydrate moiety of the AMF receptor (AMFR), which is a glycosylated seven-transmembrane helix protein. Carbohydrates 80-92 glucose-6-phosphate isomerase Homo sapiens 59-62 12027906-3 2002 METHODS: The effects of sulthiame (a) on pHi of 2",7-bis(2-carboxyethyl)-5(6)-carboxyfluorescein-acetoxymetyl ester (BCECF-AM) loaded CA3 neurones as well as (b) on epileptiform activity (induced by 50 microM 4-aminopyridine) were compared with those of the CA inhibitors acetazolamide and benzolamide. sulthiame 24-33 glucose-6-phosphate isomerase Homo sapiens 41-44 11916777-6 2002 After acute normovolemic hemodilution, pHi was significantly lower (P < 0.01) in the Control group (7.22 +/- 0.25) than in the PFC group (7.44 +/- 0.25), and CO2 gap was significantly higher (P < 0.001) in the Control group (23.4 +/- 5.1 mm Hg) than in the PFC group (1.8 +/- 0.8 mm Hg). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 161-164 glucose-6-phosphate isomerase Homo sapiens 39-42 11784758-1 2002 The role of intracellular cAMP and Ca(2+) in the excitation and adaptation of taste responses by HCl was investigated by direct measurement of intracellular pH (pH(i)) in polarized taste receptor cells (TRCs) and by chorda tympani (CT) nerve recordings. Hydrochloric Acid 97-100 glucose-6-phosphate isomerase Homo sapiens 161-166 11896299-3 2002 It has been hypothesized that intracellular formation of acetate causes a reduction of intracellular pH (pH(i)) at noncytotoxic levels, but that prolonged exposure to reduced pH(i) is cytotoxic and/or mitogenic and drives proliferative responses. Acetates 57-64 glucose-6-phosphate isomerase Homo sapiens 105-110 11896299-3 2002 It has been hypothesized that intracellular formation of acetate causes a reduction of intracellular pH (pH(i)) at noncytotoxic levels, but that prolonged exposure to reduced pH(i) is cytotoxic and/or mitogenic and drives proliferative responses. Acetates 57-64 glucose-6-phosphate isomerase Homo sapiens 175-180 11896299-7 2002 Cellular acidification was rapidly reversed to control pH(i) upon removal of vinyl acetate. vinyl acetate 77-90 glucose-6-phosphate isomerase Homo sapiens 55-60 11896299-9 2002 The effect of vinyl acetate on pH(i) was attenuated by prior exposure to the carboxylesterase inhibitor bis(p-nitrophenyl)phosphate. vinyl acetate 14-27 glucose-6-phosphate isomerase Homo sapiens 31-36 11896299-9 2002 The effect of vinyl acetate on pH(i) was attenuated by prior exposure to the carboxylesterase inhibitor bis(p-nitrophenyl)phosphate. bis(4-nitrophenyl)phosphate 104-131 glucose-6-phosphate isomerase Homo sapiens 31-36 11784807-3 2002 In this study, we sought to identify the optimal targeting sites in GPi and STN for reversal of parkinsonian signs through a series of reversible injections of the GABA(A) agonist muscimol in these nuclei in parkinsonian primates. gamma-Aminobutyric Acid 164-168 glucose-6-phosphate isomerase Homo sapiens 68-71 11784807-3 2002 In this study, we sought to identify the optimal targeting sites in GPi and STN for reversal of parkinsonian signs through a series of reversible injections of the GABA(A) agonist muscimol in these nuclei in parkinsonian primates. Muscimol 180-188 glucose-6-phosphate isomerase Homo sapiens 68-71 12440699-1 2002 Bicarbonate is important for pHi control in cardiac cells. Bicarbonates 0-11 glucose-6-phosphate isomerase Homo sapiens 29-32 12440699-3 2002 Both bicarbonate and H+/OH- transporters participate in the sarcolemmal regulation of pHi, namely Na(+)-HCO3-cotransport (NBC), Cl(-)-HCO3- exchange (i.e., anion exchange, AE), Na(+)-H+ exchange (NHE), and Cl(-)-OH- exchange (CHE). Bicarbonates 5-16 glucose-6-phosphate isomerase Homo sapiens 86-89 12440699-3 2002 Both bicarbonate and H+/OH- transporters participate in the sarcolemmal regulation of pHi, namely Na(+)-HCO3-cotransport (NBC), Cl(-)-HCO3- exchange (i.e., anion exchange, AE), Na(+)-H+ exchange (NHE), and Cl(-)-OH- exchange (CHE). Bicarbonates 104-108 glucose-6-phosphate isomerase Homo sapiens 86-89 12440699-3 2002 Both bicarbonate and H+/OH- transporters participate in the sarcolemmal regulation of pHi, namely Na(+)-HCO3-cotransport (NBC), Cl(-)-HCO3- exchange (i.e., anion exchange, AE), Na(+)-H+ exchange (NHE), and Cl(-)-OH- exchange (CHE). cholesteryl linoleate 206-214 glucose-6-phosphate isomerase Homo sapiens 86-89 12200958-9 2002 The results with the pH-insensitive probe (DHE) confirmed that oxidant production was pH-dependent. dihydroethidium 43-46 glucose-6-phosphate isomerase Homo sapiens 21-23 12200958-9 2002 The results with the pH-insensitive probe (DHE) confirmed that oxidant production was pH-dependent. dihydroethidium 43-46 glucose-6-phosphate isomerase Homo sapiens 86-88 11888741-5 2002 RESULTS: Compared to the eloHAES group, the fall in pHi was significantly greater in the gelofusine group at clamp release (7.29 vs 7.33, P=0.003) and at 4 h following clamp release (7.29 vs 7.33, P=0.03). Polygeline 89-99 glucose-6-phosphate isomerase Homo sapiens 52-55 11784758-10 2002 In polarized TRCs, basolateral exposure to ionomycin increased TRC pH(i) and activated pH(i) recovery from NH4Cl pulse by 388%. Ammonium Chloride 107-112 glucose-6-phosphate isomerase Homo sapiens 87-92 11784758-11 2002 Apical HCl stimulation induced a transient decrease in resting TRC pH(i) followed by spontaneous recovery. Hydrochloric Acid 7-10 glucose-6-phosphate isomerase Homo sapiens 67-72 11784758-12 2002 The data suggest that cAMP enhances the sour taste of strong acids by activating a Ca(2+)- and amiloride-insensitive H(+) conductance and inhibiting pH(i) recovery in TRCs. Cyclic AMP 22-26 glucose-6-phosphate isomerase Homo sapiens 149-154 11784758-12 2002 The data suggest that cAMP enhances the sour taste of strong acids by activating a Ca(2+)- and amiloride-insensitive H(+) conductance and inhibiting pH(i) recovery in TRCs. strong acids 54-66 glucose-6-phosphate isomerase Homo sapiens 149-154 11784758-5 2002 In untreated polarized TRCs, apical stimulation with HCl concentrations between 1 and 30 mM HCl induced sustained decreases in TRC pH(i). Hydrochloric Acid 53-56 glucose-6-phosphate isomerase Homo sapiens 131-136 11784758-5 2002 In untreated polarized TRCs, apical stimulation with HCl concentrations between 1 and 30 mM HCl induced sustained decreases in TRC pH(i). Hydrochloric Acid 92-95 glucose-6-phosphate isomerase Homo sapiens 131-136 11784758-6 2002 The magnitude of pH(i) decrease increased with increasing HCl concentration. Hydrochloric Acid 58-61 glucose-6-phosphate isomerase Homo sapiens 17-22 11784758-7 2002 Following treatment of the basolateral membrane with 8-CPT-cAMP the decrease in pH(i) due to apical 1 mM HCl application was significantly increased. 8-cpt 53-58 glucose-6-phosphate isomerase Homo sapiens 80-85 11784758-7 2002 Following treatment of the basolateral membrane with 8-CPT-cAMP the decrease in pH(i) due to apical 1 mM HCl application was significantly increased. Cyclic AMP 59-63 glucose-6-phosphate isomerase Homo sapiens 80-85 11784758-7 2002 Following treatment of the basolateral membrane with 8-CPT-cAMP the decrease in pH(i) due to apical 1 mM HCl application was significantly increased. Hydrochloric Acid 105-108 glucose-6-phosphate isomerase Homo sapiens 80-85 11784758-8 2002 Treatment with cAMP alone decreased resting TRC pH(i) and inhibited the recovery of pH(i) from a basolateral NH4Cl pulse by 46%. Cyclic AMP 15-19 glucose-6-phosphate isomerase Homo sapiens 48-53 11784758-8 2002 Treatment with cAMP alone decreased resting TRC pH(i) and inhibited the recovery of pH(i) from a basolateral NH4Cl pulse by 46%. Cyclic AMP 15-19 glucose-6-phosphate isomerase Homo sapiens 84-89 11784758-8 2002 Treatment with cAMP alone decreased resting TRC pH(i) and inhibited the recovery of pH(i) from a basolateral NH4Cl pulse by 46%. Ammonium Chloride 109-114 glucose-6-phosphate isomerase Homo sapiens 84-89 11784758-10 2002 In polarized TRCs, basolateral exposure to ionomycin increased TRC pH(i) and activated pH(i) recovery from NH4Cl pulse by 388%. Ionomycin 43-52 glucose-6-phosphate isomerase Homo sapiens 67-72 11784758-10 2002 In polarized TRCs, basolateral exposure to ionomycin increased TRC pH(i) and activated pH(i) recovery from NH4Cl pulse by 388%. Ionomycin 43-52 glucose-6-phosphate isomerase Homo sapiens 87-92 11862336-3 2002 This pHi recovery is highly sensitive to the anion exchange inhibitor 4,4"-diisothiocyanato-stilbene-2,2"-disulfonic acid (DIDS) and minimally sensitive to the Na+/H+ exchange inhibitor amiloride, suggesting that it is mostly due to the action of a Na+-dependent HCO3- transporter. 4,4"-diisothiocyanato-stilbene-2,2"-disulfonic acid 70-121 glucose-6-phosphate isomerase Homo sapiens 5-8 11862336-3 2002 This pHi recovery is highly sensitive to the anion exchange inhibitor 4,4"-diisothiocyanato-stilbene-2,2"-disulfonic acid (DIDS) and minimally sensitive to the Na+/H+ exchange inhibitor amiloride, suggesting that it is mostly due to the action of a Na+-dependent HCO3- transporter. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 123-127 glucose-6-phosphate isomerase Homo sapiens 5-8 11862336-3 2002 This pHi recovery is highly sensitive to the anion exchange inhibitor 4,4"-diisothiocyanato-stilbene-2,2"-disulfonic acid (DIDS) and minimally sensitive to the Na+/H+ exchange inhibitor amiloride, suggesting that it is mostly due to the action of a Na+-dependent HCO3- transporter. Amiloride 186-195 glucose-6-phosphate isomerase Homo sapiens 5-8 11862336-5 2002 Preincubation with okadaic acid stimulates the pHi recovery rate from a CO2-induced acid load in the presence of DIDS (0.002 pHu/min vs. 0.065 pHu/min), but not in the presence of amiloride. Okadaic Acid 19-31 glucose-6-phosphate isomerase Homo sapiens 47-50 11862336-5 2002 Preincubation with okadaic acid stimulates the pHi recovery rate from a CO2-induced acid load in the presence of DIDS (0.002 pHu/min vs. 0.065 pHu/min), but not in the presence of amiloride. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 72-75 glucose-6-phosphate isomerase Homo sapiens 47-50 11862336-5 2002 Preincubation with okadaic acid stimulates the pHi recovery rate from a CO2-induced acid load in the presence of DIDS (0.002 pHu/min vs. 0.065 pHu/min), but not in the presence of amiloride. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 113-117 glucose-6-phosphate isomerase Homo sapiens 47-50 11862336-5 2002 Preincubation with okadaic acid stimulates the pHi recovery rate from a CO2-induced acid load in the presence of DIDS (0.002 pHu/min vs. 0.065 pHu/min), but not in the presence of amiloride. Amiloride 180-189 glucose-6-phosphate isomerase Homo sapiens 47-50 11862336-6 2002 Okadaic acid also accelerates the pHi elevation induced by Cl- removal (0.039 pHu/min vs. 0.067 pHu/min). Okadaic Acid 0-12 glucose-6-phosphate isomerase Homo sapiens 34-37 11530213-2 2001 The final GPI precursor was identified and structurally characterized as: ethanolamine-phosphate-6Man alpha 1-2Man alpha 1-6(mannosylphosphate) Man alpha 1-4glucosamine-inositol-phospho-ceramide. phosphorylethanolamine 74-96 glucose-6-phosphate isomerase Homo sapiens 10-13 12511190-2 2002 In the present work, we studied the effects of three inhibitors of crucial mechanisms responsible for intracellular pH (pHi) regulation on hypericin phototoxicity: N-ethylmaleimide (NEM), an inhibitor of H+-ATPase, 5"-(N,N-dimethyl)-amiloride (DMA), an inhibitor of Na+/H+ exchanger, and omeprazole (OME), an inhibitor of H+K+-ATPase. hypericin 139-148 glucose-6-phosphate isomerase Homo sapiens 120-123 11758832-15 2001 After acid challenge, mucus secretion decreases, blood flow slows, and pHi returns to normal, the latter occurring via apical bicarbonate extrusion, increasing bicarbonate secretion. Bicarbonates 126-137 glucose-6-phosphate isomerase Homo sapiens 71-74 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). Water 164-169 glucose-6-phosphate isomerase Homo sapiens 160-163 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). Water 164-169 glucose-6-phosphate isomerase Homo sapiens 160-163 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). Water 164-169 glucose-6-phosphate isomerase Homo sapiens 160-163 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). Water 164-169 glucose-6-phosphate isomerase Homo sapiens 160-163 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). acetonitrile 185-197 glucose-6-phosphate isomerase Homo sapiens 51-54 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). Methanol 213-221 glucose-6-phosphate isomerase Homo sapiens 51-54 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). Hexanes 241-247 glucose-6-phosphate isomerase Homo sapiens 51-54 11577779-4 2001 SMC cAMP production was measured at normal pHi/pHo and with reduced pHi/pHo in the absence and presence of iloprost (100 pM). Cyclic AMP 4-8 glucose-6-phosphate isomerase Homo sapiens 43-46 11577779-4 2001 SMC cAMP production was measured at normal pHi/pHo and with reduced pHi/pHo in the absence and presence of iloprost (100 pM). Cyclic AMP 4-8 glucose-6-phosphate isomerase Homo sapiens 68-71 11577779-6 2001 Without iloprost, decreasing pHi/pHo increased cAMP production (40%). Cyclic AMP 47-51 glucose-6-phosphate isomerase Homo sapiens 29-32 11835452-3 2002 Cannabinoid receptors are located presynaptically on GABA terminals within the GPi, where their activation reduces GABA reuptake. gamma-Aminobutyric Acid 53-57 glucose-6-phosphate isomerase Homo sapiens 79-82 11835452-3 2002 Cannabinoid receptors are located presynaptically on GABA terminals within the GPi, where their activation reduces GABA reuptake. gamma-Aminobutyric Acid 115-119 glucose-6-phosphate isomerase Homo sapiens 79-82 11675637-1 2001 Post-exercise recovery of intracellular pH (pH(i)) assessed using phosphorus magnetic resonance spectroscopy has not been previously evaluated in its entirety due to its complex time-course and missing data points resulting from a transient loss of inorganic phosphate signal. Phosphorus 66-76 glucose-6-phosphate isomerase Homo sapiens 44-49 11675637-1 2001 Post-exercise recovery of intracellular pH (pH(i)) assessed using phosphorus magnetic resonance spectroscopy has not been previously evaluated in its entirety due to its complex time-course and missing data points resulting from a transient loss of inorganic phosphate signal. Phosphates 259-268 glucose-6-phosphate isomerase Homo sapiens 44-49 11583576-7 2001 However, the conversion of GlcN(acyl)PI to downstream mannosylated GPI intermediates in the GPI pathway was inefficient, both for GlcN(acyl)PI produced from the exogenous lipid as well as from that obtained by metabolic labelling with [(3)H]inositol. Glucosamine 27-31 glucose-6-phosphate isomerase Homo sapiens 67-70 11583576-7 2001 However, the conversion of GlcN(acyl)PI to downstream mannosylated GPI intermediates in the GPI pathway was inefficient, both for GlcN(acyl)PI produced from the exogenous lipid as well as from that obtained by metabolic labelling with [(3)H]inositol. Glucosamine 27-31 glucose-6-phosphate isomerase Homo sapiens 92-95 11583576-7 2001 However, the conversion of GlcN(acyl)PI to downstream mannosylated GPI intermediates in the GPI pathway was inefficient, both for GlcN(acyl)PI produced from the exogenous lipid as well as from that obtained by metabolic labelling with [(3)H]inositol. glcn(acyl)pi 27-39 glucose-6-phosphate isomerase Homo sapiens 67-70 11583576-7 2001 However, the conversion of GlcN(acyl)PI to downstream mannosylated GPI intermediates in the GPI pathway was inefficient, both for GlcN(acyl)PI produced from the exogenous lipid as well as from that obtained by metabolic labelling with [(3)H]inositol. glcn(acyl)pi 27-39 glucose-6-phosphate isomerase Homo sapiens 92-95 11583576-7 2001 However, the conversion of GlcN(acyl)PI to downstream mannosylated GPI intermediates in the GPI pathway was inefficient, both for GlcN(acyl)PI produced from the exogenous lipid as well as from that obtained by metabolic labelling with [(3)H]inositol. [(3)h]inositol 235-249 glucose-6-phosphate isomerase Homo sapiens 67-70 11583576-7 2001 However, the conversion of GlcN(acyl)PI to downstream mannosylated GPI intermediates in the GPI pathway was inefficient, both for GlcN(acyl)PI produced from the exogenous lipid as well as from that obtained by metabolic labelling with [(3)H]inositol. [(3)h]inositol 235-249 glucose-6-phosphate isomerase Homo sapiens 92-95 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). acifluorfen 68-79 glucose-6-phosphate isomerase Homo sapiens 51-54 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). acifluorfen 68-79 glucose-6-phosphate isomerase Homo sapiens 160-163 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). acifluorfen 68-79 glucose-6-phosphate isomerase Homo sapiens 160-163 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). acifluorfen 68-79 glucose-6-phosphate isomerase Homo sapiens 160-163 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). acifluorfen 68-79 glucose-6-phosphate isomerase Homo sapiens 160-163 11600024-4 2001 The calculated polychromatic quantum efficiencies (Phi(solvent)) of acifluorfen in different solvents are as follows (units are degraded molecules photon(-1)): Phi(water) = 10(-4), Phi(acetonitrile) = 10(-4), Phi(methanol) = 10(-4), and Phi(hexane) = 10(-2). Water 164-169 glucose-6-phosphate isomerase Homo sapiens 51-54 11575835-6 2001 In group OD, pHi was significantly decreased at OD10 (7.35 +/- 0.03 at CPB30 vs. 7.23 +/- 0.07 at OD10, p < 0.05) but recovered by the end of CPB (7.32 +/- 0.02). cpb 71-74 glucose-6-phosphate isomerase Homo sapiens 13-16 11530213-3 2001 During our investigations on the biosynthesis of the acid-labile modification, the additional mannosyl phosphate substitution, we observed that the use of the nucleotide triphosphate analogue GTP gamma S (guanosine 5-O-(thiotriphosphate)) blocks the biosynthesis of the mannosylated GPI glycolipids. guanosine 5-o-(thiotriphosphate 205-236 glucose-6-phosphate isomerase Homo sapiens 283-286 11530213-4 2001 We show that GTP gamma S inhibits the synthesis of dolichol-phosphate-mannose, which is the donor of the mannose residues for GPI biosynthesis. Guanosine Triphosphate 13-16 glucose-6-phosphate isomerase Homo sapiens 126-129 11530213-4 2001 We show that GTP gamma S inhibits the synthesis of dolichol-phosphate-mannose, which is the donor of the mannose residues for GPI biosynthesis. Sulfur 23-24 glucose-6-phosphate isomerase Homo sapiens 126-129 11530213-4 2001 We show that GTP gamma S inhibits the synthesis of dolichol-phosphate-mannose, which is the donor of the mannose residues for GPI biosynthesis. Dolichol Monophosphate Mannose 51-77 glucose-6-phosphate isomerase Homo sapiens 126-129 11530213-4 2001 We show that GTP gamma S inhibits the synthesis of dolichol-phosphate-mannose, which is the donor of the mannose residues for GPI biosynthesis. Mannose 70-77 glucose-6-phosphate isomerase Homo sapiens 126-129 11530213-6 2001 All the data obtained suggest the involvement of classical heterotrimeric G proteins in the regulation of GPI-anchor biosynthesis through dolichol-phosphate-mannose synthesis via the activation of adenylyl cyclase and protein phosphorylation. Dolichol Phosphates 138-156 glucose-6-phosphate isomerase Homo sapiens 106-109 11530213-6 2001 All the data obtained suggest the involvement of classical heterotrimeric G proteins in the regulation of GPI-anchor biosynthesis through dolichol-phosphate-mannose synthesis via the activation of adenylyl cyclase and protein phosphorylation. Mannose 157-164 glucose-6-phosphate isomerase Homo sapiens 106-109 11530213-3 2001 During our investigations on the biosynthesis of the acid-labile modification, the additional mannosyl phosphate substitution, we observed that the use of the nucleotide triphosphate analogue GTP gamma S (guanosine 5-O-(thiotriphosphate)) blocks the biosynthesis of the mannosylated GPI glycolipids. Mannosephosphates 94-112 glucose-6-phosphate isomerase Homo sapiens 283-286 11530213-3 2001 During our investigations on the biosynthesis of the acid-labile modification, the additional mannosyl phosphate substitution, we observed that the use of the nucleotide triphosphate analogue GTP gamma S (guanosine 5-O-(thiotriphosphate)) blocks the biosynthesis of the mannosylated GPI glycolipids. nucleotide triphosphate 159-182 glucose-6-phosphate isomerase Homo sapiens 283-286 11530213-3 2001 During our investigations on the biosynthesis of the acid-labile modification, the additional mannosyl phosphate substitution, we observed that the use of the nucleotide triphosphate analogue GTP gamma S (guanosine 5-O-(thiotriphosphate)) blocks the biosynthesis of the mannosylated GPI glycolipids. Guanosine Triphosphate 192-195 glucose-6-phosphate isomerase Homo sapiens 283-286 11356840-2 2001 In Saccharomyces cerevisiae, however, the GPI transferred to protein bears a fourth, alpha1,2-linked Man on the alpha1,2-Man that receives the phosphoethanolamine (EthN-P) moiety through which GPIs become linked to protein. phosphorylethanolamine 143-162 glucose-6-phosphate isomerase Homo sapiens 42-45 11356840-2 2001 In Saccharomyces cerevisiae, however, the GPI transferred to protein bears a fourth, alpha1,2-linked Man on the alpha1,2-Man that receives the phosphoethanolamine (EthN-P) moiety through which GPIs become linked to protein. ethn-p 164-170 glucose-6-phosphate isomerase Homo sapiens 42-45 11356840-3 2001 We report that temperature-sensitive smp3 mutants accumulate a GPI containing three mannoses and that smp3 is epistatic to the gpi11, gpi13, and gaa1 mutations, which normally result in the accumulation of Man(4)-GPIs, including the presumed substrate for the yeast GPI transamidase. Mannose 84-92 glucose-6-phosphate isomerase Homo sapiens 63-66 11356840-5 2001 The finding that smp3 prevents the formation of the Man(4)-GPI that accumulates when addition of EthN-P to Man-3 is blocked in a gpi13 mutant suggests that the presence of the fourth Man is important for transfer of EthN-P to Man-3 of yeast GPIs. ethn-p 97-103 glucose-6-phosphate isomerase Homo sapiens 59-62 11356840-5 2001 The finding that smp3 prevents the formation of the Man(4)-GPI that accumulates when addition of EthN-P to Man-3 is blocked in a gpi13 mutant suggests that the presence of the fourth Man is important for transfer of EthN-P to Man-3 of yeast GPIs. ethn-p 216-222 glucose-6-phosphate isomerase Homo sapiens 59-62 11356840-6 2001 The Man(3)-GPI that accumulates in smp3 is a mixture of two dominant isoforms, one bearing a single EthN-P side branch on Man-1, the other with EthN-P on Man-2, and these isoforms can be placed in separate arms of a branched GPI assembly pathway. ethn 100-104 glucose-6-phosphate isomerase Homo sapiens 11-14 11356840-6 2001 The Man(3)-GPI that accumulates in smp3 is a mixture of two dominant isoforms, one bearing a single EthN-P side branch on Man-1, the other with EthN-P on Man-2, and these isoforms can be placed in separate arms of a branched GPI assembly pathway. ethn-p 100-106 glucose-6-phosphate isomerase Homo sapiens 11-14 11677779-4 2001 The major class of modification, however, is represented by glycosylation, N-linked, O-linked, or glycosylphosphatidylinositol(GPI)-linked. Glycosylphosphatidylinositols 98-126 glucose-6-phosphate isomerase Homo sapiens 127-130 11476329-5 2001 Cellulose acetate identified more alleles at Ak and Fum, and resolved better at Pgm, whereas acrylamide identified more alleles at Gpi, Mdh, and Me. Acrylamide 93-103 glucose-6-phosphate isomerase Homo sapiens 131-134 11432820-0 2001 Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors. Acetylglucosamine 15-21 glucose-6-phosphate isomerase Homo sapiens 43-46 11264592-1 2001 Phosphoglucose isomerase (PGI) is the second enzyme in the glycolytic pathway and catalyzes an aldose-ketose isomerization. Ketoses 102-108 glucose-6-phosphate isomerase Homo sapiens 0-24 11411986-4 2001 Relative to PPh3, the effect of AsPh3 is to increase the rate of the oxidative addition of PhI by a factor ten in DMF and seven in THF, independent of the value of n, provided that n > or = 2. Dimethylformamide 114-117 glucose-6-phosphate isomerase Homo sapiens 91-94 11411986-4 2001 Relative to PPh3, the effect of AsPh3 is to increase the rate of the oxidative addition of PhI by a factor ten in DMF and seven in THF, independent of the value of n, provided that n > or = 2. tetrahydrofuran 131-134 glucose-6-phosphate isomerase Homo sapiens 91-94 11436565-0 2001 [Modification of a method for detecting the post-phoretic activity of mannose (Mpi, EC 5.3.1.8)- and glucose (Gpi, EC5.3.1.9)-6-phosphate isomerase]. Glucose 101-108 glucose-6-phosphate isomerase Homo sapiens 110-113 11307162-4 2001 Intracellular pH (pH(i)) was monitored with the fluorescent dye BCECF. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 64-69 glucose-6-phosphate isomerase Homo sapiens 18-23 11264592-1 2001 Phosphoglucose isomerase (PGI) is the second enzyme in the glycolytic pathway and catalyzes an aldose-ketose isomerization. Ketoses 102-108 glucose-6-phosphate isomerase Homo sapiens 26-29 11300380-2 2001 Thus a newly developed PDE III inhibitor, olprinone, could modify gastric intramucosal pH (pHi), systemic oxygen consumption, and systemic inflammatory responses in patients undergoing cardiac surgery with cardiopulmonary bypass (CPB). olprinone 42-51 glucose-6-phosphate isomerase Homo sapiens 91-94 11300380-8 2001 RESULTS: The pHi and PCO2-gap, the difference between PrCO2 and arterial CO2 tension (PaCO2), showed a transient decrease and an increase after CPB, respectively. prco2 54-59 glucose-6-phosphate isomerase Homo sapiens 13-16 11300380-8 2001 RESULTS: The pHi and PCO2-gap, the difference between PrCO2 and arterial CO2 tension (PaCO2), showed a transient decrease and an increase after CPB, respectively. paco2 86-91 glucose-6-phosphate isomerase Homo sapiens 13-16 11238916-7 2001 In WT PTP phi-overexpressing cells, the focal contact protein paxillin is selectively depleted from focal complexes and specifically dephosphorylated on tyrosine. Tyrosine 153-161 glucose-6-phosphate isomerase Homo sapiens 10-13 11312479-1 2001 OBJECTIVE: To investigate the usefulness of low-dose milrinone on gastric intramucosal pH (pHi) and systemic inflammation in patients undergoing hypothermic cardiopulmonary bypass (CPB). Milrinone 53-62 glucose-6-phosphate isomerase Homo sapiens 91-94 11312479-13 2001 Milrinone prevented gastric intramucosal acidosis, detected as a decrease in pHi or an increase in PCO2-gap, without affecting hepatic venous blood flow. Milrinone 0-9 glucose-6-phosphate isomerase Homo sapiens 77-80 11248096-6 2001 The potential role of pH(i) in cell viability was confirmed in Daudi cells treated with an Na(+)/H(+) exchanger inhibitor 5-(N,N-hexamethylene)amiloride. 5-(N,N-hexamethylene)amiloride 122-152 glucose-6-phosphate isomerase Homo sapiens 22-27 11275665-2 2001 A substantial part of PHI-related brain damage occurs upon reperfusion and reoxygenation by the excess production of excitatory amino acids, free (pro)radicals and the release of cytokines, triggering programmed cell death. Excitatory Amino Acids 117-139 glucose-6-phosphate isomerase Homo sapiens 22-25 11161624-3 2001 The results show stable values of basal release of the examined amino acids within 1 h. The basal levels of GABA in "OFF" state were significantly higher in the GPi than in the GPe. gamma-Aminobutyric Acid 108-112 glucose-6-phosphate isomerase Homo sapiens 161-164 11303914-7 2000 Bax immuno-reactivity was found to be related to intracellular pH (pHi), and C2-Ceramide (C2-Cer) induced a very early (<10 min) transitory increase in pHi. N-acetylsphingosine 77-88 glucose-6-phosphate isomerase Homo sapiens 155-158 11147795-4 2001 We show here for the first time that GLP-1 rapidly released free fatty acids (FFAs) from cellular stores, thereby lowering intracellular pH (pHi) and stimulating FFA oxidation in clonal beta-cells (HIT). Fatty Acids, Nonesterified 60-76 glucose-6-phosphate isomerase Homo sapiens 141-144 11303914-7 2000 Bax immuno-reactivity was found to be related to intracellular pH (pHi), and C2-Ceramide (C2-Cer) induced a very early (<10 min) transitory increase in pHi. N-acetylsphingosine 77-83 glucose-6-phosphate isomerase Homo sapiens 155-158 11007312-2 2000 Cell pH (pHi) measurements using a pH-sensitive fluorescence probe in the absence of HCO3-/CO2 revealed the presence of a Na+/H+ exchanger that required high concentrations of amiloride for full inhibition. Amiloride 176-185 glucose-6-phosphate isomerase Homo sapiens 9-12 11211125-4 2000 Acidification of cytosolic pH (pHi) increased IKs but decreased 1K whereas intracellular alkalinization decreased I(Ks) but increased IK. Potassium 47-49 glucose-6-phosphate isomerase Homo sapiens 31-34 11034090-5 2000 We have therefore compared the effects of various novel dimethanesulfonate compounds (related to BU) in terms of their toxicity to different stem cell subsets in vivo and in vitro and their ability to provide for long-term donor bone marrow engraftment using the congenic glucose-6-phosphate isomerase type 1 marker. dimethanesulfonate 56-74 glucose-6-phosphate isomerase Homo sapiens 272-301 11089752-1 2000 OBJECTIVE: To evaluate the time course of changes in the gastric mucosal pH (pHi) and the gastric mucosal to arterial CO2 gap (CO2 gap) following paracetamol-induced acute liver failure and to relate these variables to the severity of illness. Acetaminophen 146-157 glucose-6-phosphate isomerase Homo sapiens 77-80 11089752-11 2000 CONCLUSIONS: Paracetamol-induced acute liver failure is associated with increases in the CO2 gap and decreases in pHi between 4 to 9 days post-paracetamol ingestion. Acetaminophen 13-24 glucose-6-phosphate isomerase Homo sapiens 114-117 11089752-11 2000 CONCLUSIONS: Paracetamol-induced acute liver failure is associated with increases in the CO2 gap and decreases in pHi between 4 to 9 days post-paracetamol ingestion. Acetaminophen 143-154 glucose-6-phosphate isomerase Homo sapiens 114-117 11295869-12 2001 Thus, reduction in intracellular pH (pHi) is proposed as the dosimeter most closely linked to the earliest stages of vinyl acetate toxicity. vinyl acetate 117-130 glucose-6-phosphate isomerase Homo sapiens 37-40 11007312-3 2000 In the presence of HCO3-/CO2 another pHi recovery process, dependent on Na+ but independent of Cl-, was identified. Bicarbonates 19-23 glucose-6-phosphate isomerase Homo sapiens 37-40 11007312-3 2000 In the presence of HCO3-/CO2 another pHi recovery process, dependent on Na+ but independent of Cl-, was identified. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 25-28 glucose-6-phosphate isomerase Homo sapiens 37-40 11007312-5 2000 In addition, the pHi responses to Cl- removal were compatible with the presence of a Na+-independent Cl-/HCO3- exchanger that was also inhibited by DIDS. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 148-152 glucose-6-phosphate isomerase Homo sapiens 17-20 10864001-6 2000 pHi recovery from an NH4+-induced acid load was blocked by sodium removal or amiloride addition. Sodium 59-65 glucose-6-phosphate isomerase Homo sapiens 0-3 10933369-5 2000 As the index of sarcolemmal NHE activity, the rate of H+ efflux at a pHi of 6.90 J(H6.9)) was determined after the induction of intracellular acidosis in bicarbonate-free medium. Bicarbonates 154-165 glucose-6-phosphate isomerase Homo sapiens 69-72 10998877-3 2000 There were marked changes in pulse rate, PaO2, L/P ratio and gastric intramural pH (pHi) in groups PG + S, TM + S and NG + S in which sevoflurane was used compared with groups PG + P, TM + P and NG + P in which propofol was used. Alprostadil 99-105 glucose-6-phosphate isomerase Homo sapiens 84-87 10844535-11 2000 These results indicate that inhibition of annexin V binding to procoagulant phospholipid surfaces is dependent upon anti-ss2-GPI antibodies and suggest a role for annexin V in the pathogenesis of the APS. Phospholipids 76-88 glucose-6-phosphate isomerase Homo sapiens 125-128 10818071-8 2000 Aldosterone (0.5 nmol/L) rapidly increased pH(i), with a half-maximal effect between 2 and 3 nmol/L in both fetal and adult vessels. Aldosterone 0-11 glucose-6-phosphate isomerase Homo sapiens 43-48 10818071-10 2000 The hormone-mediated increase in pH(i) was unaffected by spironolactone, a classic antagonist of MR, but was completely blocked by RU28318. RU 28318 131-138 glucose-6-phosphate isomerase Homo sapiens 33-38 11004567-6 2000 The rhAMF retained the biological activities of the native AMF, i.e., catalyzes phosphohexose isomerization and stimulated cell motility. phosphohexose 80-93 glucose-6-phosphate isomerase Homo sapiens 6-9 11004567-7 2000 Additionally, we show here that human PHI is phosphorylated at serine 185 by casein kinase II (CK II) and we provide experimental evidence suggesting that this phosphorylation is associated with secretion, thus providing insights for elucidating the intracellular signal transmission of cell response to stimulation by AMF/NLK/MF. Serine 63-69 glucose-6-phosphate isomerase Homo sapiens 38-41 10910065-4 2000 Administration of MIBG (30 mg/kg) under hyperglycemic conditions (26 mM) reduced tumor pHi and pHe by approximately 0.4 (P < 0.001) and approximately 0.6 (P < 0.001) unit, respectively; coincidentally, the nucleoside triphosphates:Pi ratio decreased approximately 60% (P < 0.004) relative to the baseline level. 3-Iodobenzylguanidine 18-22 glucose-6-phosphate isomerase Homo sapiens 87-90 10864001-6 2000 pHi recovery from an NH4+-induced acid load was blocked by sodium removal or amiloride addition. Amiloride 77-86 glucose-6-phosphate isomerase Homo sapiens 0-3 10749735-3 2000 Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. Ammonium Chloride 63-70 glucose-6-phosphate isomerase Homo sapiens 11-13 10749735-3 2000 Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. co(2) 72-77 glucose-6-phosphate isomerase Homo sapiens 11-13 10749735-3 2000 Changes in pH(i) were induced by local external application of NH(4)Cl, CO(2), or sodium propionate. sodium propionate 82-99 glucose-6-phosphate isomerase Homo sapiens 11-13 10608211-7 1999 The AP disappeared only after administration of high pH lidocaine solutions (pH = 7.8, 9.0) and reappeared by washing out the solution in the chamber. Lidocaine 56-65 glucose-6-phosphate isomerase Homo sapiens 53-55 11043915-5 2000 From this measurement and from the arterial bicarbonate concentration gastric intramucosal pH (pHi) can be calculated, assuming that bicarbonate concentration in the gastric mucosal tissue is in equilibrium with systemic arterial bicarbonate. Bicarbonates 44-55 glucose-6-phosphate isomerase Homo sapiens 95-98 11043915-5 2000 From this measurement and from the arterial bicarbonate concentration gastric intramucosal pH (pHi) can be calculated, assuming that bicarbonate concentration in the gastric mucosal tissue is in equilibrium with systemic arterial bicarbonate. Bicarbonates 133-144 glucose-6-phosphate isomerase Homo sapiens 95-98 11043915-5 2000 From this measurement and from the arterial bicarbonate concentration gastric intramucosal pH (pHi) can be calculated, assuming that bicarbonate concentration in the gastric mucosal tissue is in equilibrium with systemic arterial bicarbonate. Bicarbonates 133-144 glucose-6-phosphate isomerase Homo sapiens 95-98 10916680-4 2000 GPI is a dimeric enzyme that catalyses the reversible interconversion of fructose-6-phosphate and glucose-6-phosphate. fructose-6-phosphate 73-93 glucose-6-phosphate isomerase Homo sapiens 0-3 10916680-4 2000 GPI is a dimeric enzyme that catalyses the reversible interconversion of fructose-6-phosphate and glucose-6-phosphate. Glucose 98-105 glucose-6-phosphate isomerase Homo sapiens 0-3 10666221-5 2000 Advantage was taken of this relationship by treating leukemic cells with the Na(+)/H(+) exchanger inhibitor, 5-(N, N-hexamethylene)-amiloride (HMA), which decreases the pH(i) and induces apoptosis. 5-(N,N-hexamethylene)amiloride 109-141 glucose-6-phosphate isomerase Homo sapiens 169-174 10666221-5 2000 Advantage was taken of this relationship by treating leukemic cells with the Na(+)/H(+) exchanger inhibitor, 5-(N, N-hexamethylene)-amiloride (HMA), which decreases the pH(i) and induces apoptosis. 5-(N,N-hexamethylene)amiloride 143-146 glucose-6-phosphate isomerase Homo sapiens 169-174 10666221-6 2000 By incubating patient leukemic cells in vitro with pharmacologic doses of HMA for up to 5 hours, we show, using flow cytometry and fluorescent ratio imaging microscopy, that when the pH(i) decreases, apoptosis-measured by annexin-V and TUNEL methodologies-rapidly increases so that more than 90% of the leukemic cells are killed. 5-(N,N-hexamethylene)amiloride 74-77 glucose-6-phosphate isomerase Homo sapiens 183-188 11191360-4 2000 SMC transport systems that regulate pHi include the Na+ - H+ transporter which regulates intracellular Na+ and H+ and aids in recovery from acid loads, and the Na+ -dependent and Na+ -independent Cl-/HCO3- transporters which regulate intracellular chloride. Chlorides 248-256 glucose-6-phosphate isomerase Homo sapiens 36-39 10608211-7 1999 The AP disappeared only after administration of high pH lidocaine solutions (pH = 7.8, 9.0) and reappeared by washing out the solution in the chamber. Lidocaine 56-65 glucose-6-phosphate isomerase Homo sapiens 77-79 10608211-9 1999 The mean Ci and charged lidocaine concentration in the axoplasm, when the AP disappeared or reappeared, were lower at pH 9.0 than at pH 7.8 (P < 0.05). Lidocaine 24-33 glucose-6-phosphate isomerase Homo sapiens 118-120 10548554-1 1999 Plasma membrane Cl(-)/HCO(3)(-) anion-exchange (AE) proteins contribute to regulation of intracellular pH (pH(i)). Bicarbonates 22-28 glucose-6-phosphate isomerase Homo sapiens 107-112 11970638-6 1999 The final expression for G" is related to the volume fraction of solid fat (Phi) via the mass fractal dimension (D) of the network, which agrees with the experimental verification of the scaling behavior of fat-crystal networks [S. S. Narine and A. G. Marangoni, Phys. narine 235-241 glucose-6-phosphate isomerase Homo sapiens 76-79 10579270-1 1999 OBJECTIVES: To determine the accuracy of intramucosal pH (pHi) calculated using arterial bicarbonate instead of mucosal capillary bicarbonate in the Henderson-Hasselbalch equation. Bicarbonates 89-100 glucose-6-phosphate isomerase Homo sapiens 58-61 10534758-4 1999 Next, successive chemical treatments allowed us to remove the neutral glycan moiety of thyroidal GPI, and its composition was obtained by gas chromatography. Polysaccharides 70-76 glucose-6-phosphate isomerase Homo sapiens 97-100 10534758-6 1999 Our results support the existence in porcine thyroid cells of the GPI/IPG system, which can take part in TSH-dependent signal transduction processes. 2-Isopropoxyethanol 70-73 glucose-6-phosphate isomerase Homo sapiens 66-69 10534758-6 1999 Our results support the existence in porcine thyroid cells of the GPI/IPG system, which can take part in TSH-dependent signal transduction processes. Thyrotropin 105-108 glucose-6-phosphate isomerase Homo sapiens 66-69 10602306-3 1999 For both series of benzofuran-analogues and benzothiophene-analogues, an "odd/even" relationship between the position of an oxyanion on the aromatic ring relative to the attachment point to the dioxetane and the chemiluminescent properties, lambda(max), Phi(CL), and t(1/2), is observed, as in the case for dioxetanes bearing a phenolic or naphtholic substituent. benzofuran 19-29 glucose-6-phosphate isomerase Homo sapiens 254-257 10593167-8 1999 After the administration of low-dose aspirin (40 mg/day) for about 1 month, the TX/PGI ratio decreased to around the normal level. Aspirin 37-44 glucose-6-phosphate isomerase Homo sapiens 83-86 10545154-13 1999 At pH 5.8, Popen was inhibited by 70 % and single channel conductance increased by 35 %. popen 11-16 glucose-6-phosphate isomerase Homo sapiens 3-5 10593168-6 1999 Some of the patients who had relatively low levels of blood glucose also showed high TX/PGI ratios. Glucose 60-67 glucose-6-phosphate isomerase Homo sapiens 88-91 10473043-6 1999 Using univariate linear regression, we determined that increased lactate/creatine plus phosphocreatine (Cr) was associated with an alkaline intracellular pH (pHi) (p < 0.001) and increased inorganic phosphate/phosphocreatine (Pi/PCr) (p < 0.001). Lactic Acid 65-72 glucose-6-phosphate isomerase Homo sapiens 158-161 10596888-11 1999 The median base line oxygen saturation was 88 and 93%, respectively in Gp I and II pre-operatively and improved to 90.1 and 99.2%, post-operatively. Oxygen 21-27 glucose-6-phosphate isomerase Homo sapiens 71-82 10548200-0 1999 Dopexamine increases splanchnic blood flow but decreases gastric mucosal pH in severe septic patients treated with dobutamine. dopexamine 0-10 glucose-6-phosphate isomerase Homo sapiens 73-75 10548200-0 1999 Dopexamine increases splanchnic blood flow but decreases gastric mucosal pH in severe septic patients treated with dobutamine. Dobutamine 115-125 glucose-6-phosphate isomerase Homo sapiens 73-75 10548200-10 1999 Dopexamine decreased pHi in a dose-dependent fashion in all 12 patients. dopexamine 0-10 glucose-6-phosphate isomerase Homo sapiens 21-24 10487777-7 1999 H(+)/solute-induced acidification (using glycylsarcosine or beta-alanine) led to Na(+)-dependent, EIPA-inhibitable pH(i) recovery or EIPA-inhibitable (22)Na(+) influx at the apical membrane only. glycylsarcosine 41-56 glucose-6-phosphate isomerase Homo sapiens 115-120 10487777-7 1999 H(+)/solute-induced acidification (using glycylsarcosine or beta-alanine) led to Na(+)-dependent, EIPA-inhibitable pH(i) recovery or EIPA-inhibitable (22)Na(+) influx at the apical membrane only. beta-Alanine 60-72 glucose-6-phosphate isomerase Homo sapiens 115-120 10487777-7 1999 H(+)/solute-induced acidification (using glycylsarcosine or beta-alanine) led to Na(+)-dependent, EIPA-inhibitable pH(i) recovery or EIPA-inhibitable (22)Na(+) influx at the apical membrane only. ethylisopropylamiloride 98-102 glucose-6-phosphate isomerase Homo sapiens 115-120 10458740-2 1999 Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammonium from the medium of cultured mammalian cells. udp-n-acetylhexosamine 120-142 glucose-6-phosphate isomerase Homo sapiens 0-33 10458740-2 1999 Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammonium from the medium of cultured mammalian cells. udp-n-acetylhexosamine 120-142 glucose-6-phosphate isomerase Homo sapiens 35-38 10458740-2 1999 Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammonium from the medium of cultured mammalian cells. Ammonium Compounds 171-179 glucose-6-phosphate isomerase Homo sapiens 0-33 10458740-2 1999 Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc) by accepting ammonium from the medium of cultured mammalian cells. Ammonium Compounds 171-179 glucose-6-phosphate isomerase Homo sapiens 35-38 10458740-6 1999 To reduce the negative effects of ammonium, GPI was inhibited using two different strategies. Ammonium Compounds 34-42 glucose-6-phosphate isomerase Homo sapiens 44-47 10458740-11 1999 GPI was induced to a factor of two under ammonium-containing medium conditions. Ammonium Compounds 41-49 glucose-6-phosphate isomerase Homo sapiens 0-3 10458740-12 1999 We propose gene knockout technology for GPI repression to obtain cell lines consisting of an UDPGNAc pool unaffected by the presence of ammonium. Uridine Diphosphate N-Acetylglucosamine 93-100 glucose-6-phosphate isomerase Homo sapiens 40-43 10522826-3 1999 Measurement of pHi with the ratiometric dye BCECF provided resolution similar to that of a microscopic approach. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 44-49 glucose-6-phosphate isomerase Homo sapiens 15-18 10521142-6 1999 Inhibition of membrane transporters responsible for pHi regulation (0.1 mM amiloride for the Na+/H+ antiporter or 1 mM SITS for HCO3- -dependent transporters) inhibited cell swelling from acidosis but did not affect the profound intracellular acidification. Amiloride 75-84 glucose-6-phosphate isomerase Homo sapiens 52-55 10473043-6 1999 Using univariate linear regression, we determined that increased lactate/creatine plus phosphocreatine (Cr) was associated with an alkaline intracellular pH (pHi) (p < 0.001) and increased inorganic phosphate/phosphocreatine (Pi/PCr) (p < 0.001). Chromium 104-106 glucose-6-phosphate isomerase Homo sapiens 158-161 10473043-6 1999 Using univariate linear regression, we determined that increased lactate/creatine plus phosphocreatine (Cr) was associated with an alkaline intracellular pH (pHi) (p < 0.001) and increased inorganic phosphate/phosphocreatine (Pi/PCr) (p < 0.001). Phosphocreatine 87-102 glucose-6-phosphate isomerase Homo sapiens 158-161 10362747-0 1999 Basolateral regulation of pHi in isolated snake renal proximal tubules in presence and absence of bicarbonate. Bicarbonates 98-109 glucose-6-phosphate isomerase Homo sapiens 26-29 10417409-8 1999 The Ramachandran (Phi,Psi) angles around Gly in the consensus sequence show clustering in the region which is disallowed for non-glycyl residues. Glycine 41-44 glucose-6-phosphate isomerase Homo sapiens 18-21 10417409-8 1999 The Ramachandran (Phi,Psi) angles around Gly in the consensus sequence show clustering in the region which is disallowed for non-glycyl residues. glycyl 129-135 glucose-6-phosphate isomerase Homo sapiens 18-21 10417409-10 1999 For the 44 confirmed N-glycosylating sequences, an in-depth analysis of the (Psi(N), Phi(X), Psi(X), Phi(S/T)) dihedral angles, which position the side chains of Asn and Ser/Thr, shows that these can be grouped into nine conformational states. Asparagine 162-165 glucose-6-phosphate isomerase Homo sapiens 85-88 10645027-6 1999 Serum creatinine levels and creatinine clearance correlated with pHi in the patient"s group. Creatinine 6-16 glucose-6-phosphate isomerase Homo sapiens 65-68 10645027-6 1999 Serum creatinine levels and creatinine clearance correlated with pHi in the patient"s group. Creatinine 28-38 glucose-6-phosphate isomerase Homo sapiens 65-68 10398853-1 1999 Hypercapnia as well as lowered intracellular pH (pHi) increase the bioelectric activity of CO2/H+-sensitive neurones (VLNcs) of the ventrolateral medulla oblongata. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 91-94 glucose-6-phosphate isomerase Homo sapiens 49-52 10404646-0 1999 Basolateral regulation of pHi in proximal tubules of avian loopless and long-looped nephrons in bicarbonate. Bicarbonates 96-107 glucose-6-phosphate isomerase Homo sapiens 26-29 10404646-2 1999 The rate of recovery of pHi (dpHi/dt) from this level to the resting level in proximal tubules from both nephron types was (1) significantly reduced by the removal of Na+ or both Na+ and Cl- from the bath, and (2) unaffected by the removal of Cl- from the bath or the presence of a high K+ concentration or Ba2+ in the bath. N-methyl-valyl-amiclenomycin 307-311 glucose-6-phosphate isomerase Homo sapiens 24-27 10411690-5 1999 The oral administration of 100 g glucose significantly increased the Na+/H+ antiporter activity in CRF patients from 13.35 +/- 1.26 x 10-3 pHi/second to 16.44 +/- 1.37 x 10-3 pHi/second after one hour and to 14.06 +/- 1.36 x 10-3 pHi/second after two hours (mean +/- SEM, P = 0.008 by Friedmans"s two-way analysis of variance). Glucose 33-40 glucose-6-phosphate isomerase Homo sapiens 139-142 10411690-5 1999 The oral administration of 100 g glucose significantly increased the Na+/H+ antiporter activity in CRF patients from 13.35 +/- 1.26 x 10-3 pHi/second to 16.44 +/- 1.37 x 10-3 pHi/second after one hour and to 14.06 +/- 1.36 x 10-3 pHi/second after two hours (mean +/- SEM, P = 0.008 by Friedmans"s two-way analysis of variance). Glucose 33-40 glucose-6-phosphate isomerase Homo sapiens 175-178 10411690-5 1999 The oral administration of 100 g glucose significantly increased the Na+/H+ antiporter activity in CRF patients from 13.35 +/- 1.26 x 10-3 pHi/second to 16.44 +/- 1.37 x 10-3 pHi/second after one hour and to 14.06 +/- 1.36 x 10-3 pHi/second after two hours (mean +/- SEM, P = 0.008 by Friedmans"s two-way analysis of variance). Glucose 33-40 glucose-6-phosphate isomerase Homo sapiens 175-178 10411690-6 1999 In controls, the administration of 100 g glucose significantly increased the Na+/H+ antiporter activity from 4.23 +/- 0.20 x 10-3 pHi/second to 6.00 +/- 0.56 x 10-3 pHi/second after one hour and to 6.65 +/- 0.64 x 10-3 pHi/second after two hours (P = 0.0003). Glucose 41-48 glucose-6-phosphate isomerase Homo sapiens 130-133 10411690-6 1999 In controls, the administration of 100 g glucose significantly increased the Na+/H+ antiporter activity from 4.23 +/- 0.20 x 10-3 pHi/second to 6.00 +/- 0.56 x 10-3 pHi/second after one hour and to 6.65 +/- 0.64 x 10-3 pHi/second after two hours (P = 0.0003). Glucose 41-48 glucose-6-phosphate isomerase Homo sapiens 165-168 10411690-6 1999 In controls, the administration of 100 g glucose significantly increased the Na+/H+ antiporter activity from 4.23 +/- 0.20 x 10-3 pHi/second to 6.00 +/- 0.56 x 10-3 pHi/second after one hour and to 6.65 +/- 0.64 x 10-3 pHi/second after two hours (P = 0.0003). Glucose 41-48 glucose-6-phosphate isomerase Homo sapiens 165-168 10362747-3 1999 pHi was measured with the pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) under resting conditions and in response to NH4Cl pulse. 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein 55-103 glucose-6-phosphate isomerase Homo sapiens 0-3 10362747-3 1999 pHi was measured with the pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) under resting conditions and in response to NH4Cl pulse. bcecf 105-110 glucose-6-phosphate isomerase Homo sapiens 0-3 10362747-3 1999 pHi was measured with the pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) under resting conditions and in response to NH4Cl pulse. Ammonium Chloride 156-161 glucose-6-phosphate isomerase Homo sapiens 0-3 10362747-4 1999 Resting pHi (approximately 7.1-7.2) and its response to and rate of recovery (dpHi/dt) from an NH4Cl pulse were not affected by the presence or absence of HCO-3 in either segment. Ammonium Chloride 95-100 glucose-6-phosphate isomerase Homo sapiens 8-11 10208742-7 1999 When the pHi of unfertilized eggs was elevated by exposure to 15 mM ammonium chloride in pH 9 seawater, V0 increased to a level comparable to that measured after fertilization. Ammonium Chloride 68-85 glucose-6-phosphate isomerase Homo sapiens 9-12 10513074-7 1999 Dobutamine infusion, but not amrinone, increased gastric pHi, as well as cardiac index and oxygen delivery. Dobutamine 0-10 glucose-6-phosphate isomerase Homo sapiens 57-60 10513074-8 1999 CONCLUSIONS: An improvement in gastric pHi associated with an increase in oxygen delivery, was observed with dobutamine. Oxygen 74-80 glucose-6-phosphate isomerase Homo sapiens 39-42 10513074-8 1999 CONCLUSIONS: An improvement in gastric pHi associated with an increase in oxygen delivery, was observed with dobutamine. Dobutamine 109-119 glucose-6-phosphate isomerase Homo sapiens 39-42 10796074-9 1999 In addition, in all the cell lines, HCO3- slightly acidified pHi and increased the rates of pHi recovery after an acid load, indicating the presence of anion exchanger (AE) activity. Bicarbonates 36-40 glucose-6-phosphate isomerase Homo sapiens 61-64 10796074-9 1999 In addition, in all the cell lines, HCO3- slightly acidified pHi and increased the rates of pHi recovery after an acid load, indicating the presence of anion exchanger (AE) activity. Bicarbonates 36-40 glucose-6-phosphate isomerase Homo sapiens 92-95 10404735-3 1999 Platelet intracellular pH (pHi) was measured using the fluorescent dye BCECF to monitor intracellular pH. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 71-76 glucose-6-phosphate isomerase Homo sapiens 27-30 10404735-4 1999 Sodium/hydrogen exchanger activity was estimated from the recovery of pHi clamped to 6.25 with nigericin. Hydrogen 7-15 glucose-6-phosphate isomerase Homo sapiens 70-73 10404735-4 1999 Sodium/hydrogen exchanger activity was estimated from the recovery of pHi clamped to 6.25 with nigericin. Nigericin 95-104 glucose-6-phosphate isomerase Homo sapiens 70-73 10404735-11 1999 The pHi recovery after acidification was sodium-dependent and inhibited by N-hexamethylene amiloride. Sodium 41-47 glucose-6-phosphate isomerase Homo sapiens 4-7 10404735-11 1999 The pHi recovery after acidification was sodium-dependent and inhibited by N-hexamethylene amiloride. n-hexamethylene amiloride 75-100 glucose-6-phosphate isomerase Homo sapiens 4-7 10069995-4 1999 In the nominal absence of CO2/HCO-3, exposing cell populations to a HEPES-buffered solution supplemented with approximately 300 mM mannitol (600 mosmol/kgH2O) causes steady-state pHi to increase by approximately 0.4. HEPES 68-73 glucose-6-phosphate isomerase Homo sapiens 179-182 10197642-5 1999 Clamping of pHi at 7.25 by the proton-ionophore nigericin abolished SST-signaled apoptosis without affecting its ability to regulate SHP-1, p53, and Bax. Nigericin 48-57 glucose-6-phosphate isomerase Homo sapiens 12-15 10197642-6 1999 Apoptosis could be induced by nigericin clamping of pHi to 6.5. Nigericin 30-39 glucose-6-phosphate isomerase Homo sapiens 52-55 10069984-6 1999 In Xenopus oocytes expressing hhNBC, adding 1.5% CO2/10 mM HCO-3 hyperpolarizes the membrane and causes a rapid fall in intracellular pH (pHi), followed by a pHi recovery. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 49-52 glucose-6-phosphate isomerase Homo sapiens 138-141 10069984-6 1999 In Xenopus oocytes expressing hhNBC, adding 1.5% CO2/10 mM HCO-3 hyperpolarizes the membrane and causes a rapid fall in intracellular pH (pHi), followed by a pHi recovery. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 49-52 glucose-6-phosphate isomerase Homo sapiens 158-161 10334158-13 1999 pHi decreased from 7.33 +/- 0.08 (mean +/- SD) following resuscitation to 7.26 +/- 0.04 at 24 h, 7.20 +/- 0.07 at 36 h (p < 0.05), and 7.24 +/- 0.08 at 48 h. Corresponding statistically significant and clinically relevant changes in systemic hemodynamic, oxygen utilization, and acid-base variables were not observed. Oxygen 258-264 glucose-6-phosphate isomerase Homo sapiens 0-3 10196165-11 1999 Continuous photometric monitoring of 2",7"-bis(carboxyethyl)-5, 6-carboxyfluorescein (BCECF), to assess changes in pHi or efflux of the probe through MAC pores, in single cells or cell populations, respectively, verifies changes in pHi upon CO2 conditioning and Cl- substitution and release of BCECF upon formation of MAC pores. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 37-84 glucose-6-phosphate isomerase Homo sapiens 115-118 10196165-11 1999 Continuous photometric monitoring of 2",7"-bis(carboxyethyl)-5, 6-carboxyfluorescein (BCECF), to assess changes in pHi or efflux of the probe through MAC pores, in single cells or cell populations, respectively, verifies changes in pHi upon CO2 conditioning and Cl- substitution and release of BCECF upon formation of MAC pores. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 37-84 glucose-6-phosphate isomerase Homo sapiens 232-235 10196165-11 1999 Continuous photometric monitoring of 2",7"-bis(carboxyethyl)-5, 6-carboxyfluorescein (BCECF), to assess changes in pHi or efflux of the probe through MAC pores, in single cells or cell populations, respectively, verifies changes in pHi upon CO2 conditioning and Cl- substitution and release of BCECF upon formation of MAC pores. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 86-91 glucose-6-phosphate isomerase Homo sapiens 232-235 10365907-8 1999 IP was unaltered, and pH(I) decreased as hydrogen ions produced during bicarbonate secretion were dissipated (7.41 +/- 0.01 versus 7.38 +/- 0.01, P < 0.05). Hydrogen 41-49 glucose-6-phosphate isomerase Homo sapiens 22-27 10365907-8 1999 IP was unaltered, and pH(I) decreased as hydrogen ions produced during bicarbonate secretion were dissipated (7.41 +/- 0.01 versus 7.38 +/- 0.01, P < 0.05). Bicarbonates 71-82 glucose-6-phosphate isomerase Homo sapiens 22-27 10069984-6 1999 In Xenopus oocytes expressing hhNBC, adding 1.5% CO2/10 mM HCO-3 hyperpolarizes the membrane and causes a rapid fall in intracellular pH (pHi), followed by a pHi recovery. Bicarbonates 59-64 glucose-6-phosphate isomerase Homo sapiens 138-141 10069984-6 1999 In Xenopus oocytes expressing hhNBC, adding 1.5% CO2/10 mM HCO-3 hyperpolarizes the membrane and causes a rapid fall in intracellular pH (pHi), followed by a pHi recovery. Bicarbonates 59-64 glucose-6-phosphate isomerase Homo sapiens 158-161 10069995-4 1999 In the nominal absence of CO2/HCO-3, exposing cell populations to a HEPES-buffered solution supplemented with approximately 300 mM mannitol (600 mosmol/kgH2O) causes steady-state pHi to increase by approximately 0.4. Mannitol 131-139 glucose-6-phosphate isomerase Homo sapiens 179-182 9972765-2 1999 The maximal intramucosal pH (pHi) decrease was significantly (P < 0.001) greater in the control group (0.16 +/- 0.04) than in the thoracic epidural anesthesia (TEA) group (0.07 +/- 0.05). tea 163-166 glucose-6-phosphate isomerase Homo sapiens 29-32 10206659-7 1999 Injection of buffer (0/6 oocytes) or fructose-6-phosphate, a product of GPI enzymatic reaction (0/5 oocytes), also failed to initiate Ca2+ responses. fructose-6-phosphate 37-57 glucose-6-phosphate isomerase Homo sapiens 72-75 10069937-8 1999 A subset of factors &Gammai unconditionally selectively influences Xi if the latter can be presented as a deterministic function of &Gammai and of some random variables (the same for all Xi, i=1, em leader, n) whose joint distribution does not depend on any factors from Phi. Adenosine Monophosphate 21-24 glucose-6-phosphate isomerase Homo sapiens 279-282 10069937-8 1999 A subset of factors &Gammai unconditionally selectively influences Xi if the latter can be presented as a deterministic function of &Gammai and of some random variables (the same for all Xi, i=1, em leader, n) whose joint distribution does not depend on any factors from Phi. Adenosine Monophosphate 137-140 glucose-6-phosphate isomerase Homo sapiens 279-282 9972765-5 1999 The study data show that TEA prevents the decrease of pHi during major abdominal surgery, perhaps as an effect of stable visceral perfusion. tea 25-28 glucose-6-phosphate isomerase Homo sapiens 54-57 9890750-2 1999 Moreover, Na(-)0 depletion resulted in a decreased cytosolic pH (pHi), suggesting involvement of a Na(+)-dependent pHi regulatory mechanism during the P-initiated AR. na(-)0 10-16 glucose-6-phosphate isomerase Homo sapiens 65-68 10050217-5 1999 The combination of low-dose dopamine and a low bypass flow rate was associated with a significantly greater frequency and severity of low pHi. Dopamine 28-36 glucose-6-phosphate isomerase Homo sapiens 138-141 10327616-5 1999 During VA perfusion, pHi (6.73 +/- 0.01) was significantly higher than that during LA perfusion (pHi 6.69 +/- 0.013), but the difference is probably too small to have physiological significance. Vanillic Acid 7-9 glucose-6-phosphate isomerase Homo sapiens 21-24 9890750-2 1999 Moreover, Na(-)0 depletion resulted in a decreased cytosolic pH (pHi), suggesting involvement of a Na(+)-dependent pHi regulatory mechanism during the P-initiated AR. na(-)0 10-16 glucose-6-phosphate isomerase Homo sapiens 115-118 9890750-3 1999 We now report that the decreased pHi resulting from Na(+)0 depletion is reversible and mediated by a Na+/H+ exchange (NHE) mechanism. na(+)0 52-58 glucose-6-phosphate isomerase Homo sapiens 33-36 9890750-7 1999 The steady-state pHi was then determined by spectrofluorometric measurement of bis(carboxyethyl)5(6)-carboxyfluoroscein (BCECF) fluorescence. bis(carboxyethyl)5(6)-carboxyfluoroscein 79-119 glucose-6-phosphate isomerase Homo sapiens 17-20 9890750-7 1999 The steady-state pHi was then determined by spectrofluorometric measurement of bis(carboxyethyl)5(6)-carboxyfluoroscein (BCECF) fluorescence. bcecf 121-126 glucose-6-phosphate isomerase Homo sapiens 17-20 9890750-8 1999 EIPA (0.1 microM) significantly (P < 0.05) inhibited the pHi recovery produced by NaH medium. ethylisopropylamiloride 0-4 glucose-6-phosphate isomerase Homo sapiens 60-63 9890750-8 1999 EIPA (0.1 microM) significantly (P < 0.05) inhibited the pHi recovery produced by NaH medium. nah medium 85-95 glucose-6-phosphate isomerase Homo sapiens 60-63 9890750-9 1999 Moreover, the pHi in NaH medium was not significantly (P < 0.05) different than NaB medium. nah medium 21-31 glucose-6-phosphate isomerase Homo sapiens 14-17 9890750-10 1999 These results indicate that a Na(+)-dependent, bicarbonate-independent pHi regulatory mechanism, with a pharmacological characteristic consistent with an NHE, is present in capacitated spermatozoa. Bicarbonates 47-58 glucose-6-phosphate isomerase Homo sapiens 71-74 10370901-4 1999 Reduced dopaminergic input causes overactivity of the GABA ergic inhibitory striatal neurons projecting via the "indirect loop" to SN zona reticulata (SNZR) and medial pallidum (GPI) leading to inhibition of the glutamatergic thalamo-cortical motor loop and reduced cortical activation. gamma-Aminobutyric Acid 54-58 glucose-6-phosphate isomerase Homo sapiens 178-181 9990741-7 1999 It is shown that under aerobic conditions the lactate-/H+ symporter could be the most active exchanger in the regulation of pHi in tumour cells. Lactic Acid 46-53 glucose-6-phosphate isomerase Homo sapiens 124-127 9915315-6 1999 RESULTS: Milrinone did not prevent gastrointestinal acidosis as measured by pHi, but its perioperative administration resulted in significantly higher pHi levels compared with control. Milrinone 9-18 glucose-6-phosphate isomerase Homo sapiens 151-154 16021880-6 1999 A pilot study to assess the antiviral effect of a combination of didanosine plus stavudine plus nevirapine with or without hydroxyurea in the treatment of PHI is currently under way. Didanosine 65-75 glucose-6-phosphate isomerase Homo sapiens 155-158 16021880-6 1999 A pilot study to assess the antiviral effect of a combination of didanosine plus stavudine plus nevirapine with or without hydroxyurea in the treatment of PHI is currently under way. Stavudine 81-90 glucose-6-phosphate isomerase Homo sapiens 155-158 16021880-6 1999 A pilot study to assess the antiviral effect of a combination of didanosine plus stavudine plus nevirapine with or without hydroxyurea in the treatment of PHI is currently under way. Nevirapine 96-106 glucose-6-phosphate isomerase Homo sapiens 155-158 11938765-1 1999 A method for the assay of glycosylphosphatidylinositol-specific phospholipase D(GPI-PLD) activity in human serum was established by using glycosylphosphatidylinositol(GPI) anchored placental alkaline phosphatase(PLAP) as a substrate. Glycosylphosphatidylinositols 26-54 glucose-6-phosphate isomerase Homo sapiens 80-83 11938765-1 1999 A method for the assay of glycosylphosphatidylinositol-specific phospholipase D(GPI-PLD) activity in human serum was established by using glycosylphosphatidylinositol(GPI) anchored placental alkaline phosphatase(PLAP) as a substrate. Glycosylphosphatidylinositols 26-54 glucose-6-phosphate isomerase Homo sapiens 167-170 11938765-1 1999 A method for the assay of glycosylphosphatidylinositol-specific phospholipase D(GPI-PLD) activity in human serum was established by using glycosylphosphatidylinositol(GPI) anchored placental alkaline phosphatase(PLAP) as a substrate. Glycosylphosphatidylinositols 138-166 glucose-6-phosphate isomerase Homo sapiens 80-83 11938765-1 1999 A method for the assay of glycosylphosphatidylinositol-specific phospholipase D(GPI-PLD) activity in human serum was established by using glycosylphosphatidylinositol(GPI) anchored placental alkaline phosphatase(PLAP) as a substrate. Glycosylphosphatidylinositols 138-166 glucose-6-phosphate isomerase Homo sapiens 167-170 9860472-11 1998 Jejunal intramucosal pH (pHi) was calculated every hour by the luminal PCO2, obtained with a balloon tonometer, and arterial bicarbonate concentration. pco2 71-75 glucose-6-phosphate isomerase Homo sapiens 25-28 9860472-11 1998 Jejunal intramucosal pH (pHi) was calculated every hour by the luminal PCO2, obtained with a balloon tonometer, and arterial bicarbonate concentration. Bicarbonates 125-136 glucose-6-phosphate isomerase Homo sapiens 25-28 9878595-10 1998 Treatment of T-cells with HgCl2 resulted in reduced pHi from 7.0 to 6.7. Mercuric Chloride 26-31 glucose-6-phosphate isomerase Homo sapiens 52-55 9855559-0 1998 Antiparkinsonian and anti-levodopa-induced dyskinesia effects obtained by stimulating the same site within the GPi in PD. Levodopa 26-34 glucose-6-phosphate isomerase Homo sapiens 111-114 9776724-7 1998 pHi was measured by epifluorescence with the acetoxymethyl ester form of the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF-AM). acetoxymethyl ester 45-64 glucose-6-phosphate isomerase Homo sapiens 0-3 9813142-7 1998 In an in vitro enzyme study, the NO donor sodium nitroprusside (SNP) suppressed phosphofructokinase activity and activated glucokinase and glucose-6-phosphate isomerase activity, but SNP significantly inhibited the combined activity of the enzymes. Nitroprusside 42-62 glucose-6-phosphate isomerase Homo sapiens 139-168 9824078-14 1998 Gastric pHi can be a useful measurement in the immediate posttransplantation period for differentiating between hyperlactacidemia produced by liver dysfunction (normal pHi) and hyperlactacidemia produced by lactate generation as a consequence of inadequate tissue oxygenation or of a mixed origin (abnormal pHi). Lactic Acid 207-214 glucose-6-phosphate isomerase Homo sapiens 8-11 9863995-4 1998 In tracheal strips precontracted with carbachol, hypocapnic challenge (0% CO2) produced increases in tension, pHi, and [Ca2+]i. Carbachol 38-47 glucose-6-phosphate isomerase Homo sapiens 110-113 9863995-4 1998 In tracheal strips precontracted with carbachol, hypocapnic challenge (0% CO2) produced increases in tension, pHi, and [Ca2+]i. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 74-77 glucose-6-phosphate isomerase Homo sapiens 110-113 9852690-15 1998 By multiple regression analysis, pHi was not affected significantly by CI, while it showed positive correlation with pHa, Hb, Sao2 and negative correlation with blood temperature. sao2 126-130 glucose-6-phosphate isomerase Homo sapiens 33-36 9852690-16 1998 When cardiac output increased, blood pH decreased due to increased Pco2 and decreased BE. pco2 67-71 glucose-6-phosphate isomerase Homo sapiens 37-39 9852690-16 1998 When cardiac output increased, blood pH decreased due to increased Pco2 and decreased BE. Beryllium 86-88 glucose-6-phosphate isomerase Homo sapiens 37-39 9820376-5 1998 RESULTS: Resting pHi in bicarbonate-free N"-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid was 7.5 +/- 0.03 (n = 50). Bicarbonates 24-35 glucose-6-phosphate isomerase Homo sapiens 17-20 9820376-5 1998 RESULTS: Resting pHi in bicarbonate-free N"-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid was 7.5 +/- 0.03 (n = 50). HEPES 41-93 glucose-6-phosphate isomerase Homo sapiens 17-20 9820376-6 1998 Acidification using the NH4Cl prepulse technique lowered pHi by 0.6 +/- 0.02 pH units, with recovery ensuing at an initial rate of 0.09 +/- 0.04 pH units/min. Ammonium Chloride 24-29 glucose-6-phosphate isomerase Homo sapiens 57-60 9820376-7 1998 Notably, the rate of recovery was faster the more acidic the pHi, and recovery was abolished by amiloride or replacement with an Na+-free buffer. Amiloride 96-105 glucose-6-phosphate isomerase Homo sapiens 61-64 9776724-7 1998 pHi was measured by epifluorescence with the acetoxymethyl ester form of the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF-AM). 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein 94-142 glucose-6-phosphate isomerase Homo sapiens 0-3 9776724-7 1998 pHi was measured by epifluorescence with the acetoxymethyl ester form of the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF-AM). 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 144-152 glucose-6-phosphate isomerase Homo sapiens 0-3 9781735-7 1998 Data on the effects of dopexamine on pHi are scarce and inconsistent. dopexamine 23-33 glucose-6-phosphate isomerase Homo sapiens 37-40 9781735-8 1998 Dobutamine can significantly increase SBF and usually increases pHi. Dobutamine 0-10 glucose-6-phosphate isomerase Homo sapiens 64-67 9781735-9 1998 In septic patients, norepinephrine seems to increase pHi. Norepinephrine 20-34 glucose-6-phosphate isomerase Homo sapiens 53-56 9781735-11 1998 Prostacyclin seems to increase pHi but data are limited. Epoprostenol 0-12 glucose-6-phosphate isomerase Homo sapiens 31-34 9781735-12 1998 Insufficient evidence exists to support the beneficial effects of nitric oxide donors or blockers, pentoxifylline, or N-acetylcysteine on pHi. Nitric Oxide 66-78 glucose-6-phosphate isomerase Homo sapiens 138-141 9781735-12 1998 Insufficient evidence exists to support the beneficial effects of nitric oxide donors or blockers, pentoxifylline, or N-acetylcysteine on pHi. Acetylcysteine 118-134 glucose-6-phosphate isomerase Homo sapiens 138-141 9781735-14 1998 Among the catecholamines, dopamine is the least likely, and dobutamine the most likely, to increase pHi. Catecholamines 10-24 glucose-6-phosphate isomerase Homo sapiens 100-103 9781735-14 1998 Among the catecholamines, dopamine is the least likely, and dobutamine the most likely, to increase pHi. Dopamine 26-34 glucose-6-phosphate isomerase Homo sapiens 100-103 9781735-14 1998 Among the catecholamines, dopamine is the least likely, and dobutamine the most likely, to increase pHi. Dobutamine 60-70 glucose-6-phosphate isomerase Homo sapiens 100-103 9746112-5 1998 MATERIALS AND METHODS: pHi was determined in cell suspensions using 2,7-biscarboxyethyl-5(6)-carboxyfluorescein tetraacetoxymethyl ester, a fluorescent pH indicator. 2,7-biscarboxyethyl-5(6)-carboxyfluorescein tetraacetoxymethyl ester 68-136 glucose-6-phosphate isomerase Homo sapiens 23-26 9829357-5 1998 Intracellular pH (pHi) was measured by the pH-sensitive dye BCECF and dynamic fluorescence ratio imaging. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 60-65 glucose-6-phosphate isomerase Homo sapiens 18-21 9829357-7 1998 In HCO3--free media, pHi recovery after acidification with NH4Cl was amiloride-sensitive and Na+-dependent, indicating the presence of an Na+/H+ exchanger. Bicarbonates 3-7 glucose-6-phosphate isomerase Homo sapiens 21-24 9829357-7 1998 In HCO3--free media, pHi recovery after acidification with NH4Cl was amiloride-sensitive and Na+-dependent, indicating the presence of an Na+/H+ exchanger. Ammonium Chloride 59-64 glucose-6-phosphate isomerase Homo sapiens 21-24 9829357-7 1998 In HCO3--free media, pHi recovery after acidification with NH4Cl was amiloride-sensitive and Na+-dependent, indicating the presence of an Na+/H+ exchanger. Amiloride 69-78 glucose-6-phosphate isomerase Homo sapiens 21-24 9829357-8 1998 pHi recovery after acidification was significantly enhanced by the presence of HCO3-, showing the presence of an HCO3--dependent recovery mechanism (that is, a base loader/acid extruder). Bicarbonates 79-83 glucose-6-phosphate isomerase Homo sapiens 0-3 9829357-8 1998 pHi recovery after acidification was significantly enhanced by the presence of HCO3-, showing the presence of an HCO3--dependent recovery mechanism (that is, a base loader/acid extruder). Bicarbonates 113-117 glucose-6-phosphate isomerase Homo sapiens 0-3 9746112-5 1998 MATERIALS AND METHODS: pHi was determined in cell suspensions using 2,7-biscarboxyethyl-5(6)-carboxyfluorescein tetraacetoxymethyl ester, a fluorescent pH indicator. 2,7-biscarboxyethyl-5(6)-carboxyfluorescein tetraacetoxymethyl ester 68-136 glucose-6-phosphate isomerase Homo sapiens 23-25 9688606-3 1998 However, replacement of external Cl- with gluconate caused an H2DIDS-inhibitable (100 microM) increase in the pHi of HCMV-infected cells but not in mock-infected cells. gluconic acid 42-51 glucose-6-phosphate isomerase Homo sapiens 110-113 9821105-3 1998 Intracellular pH (pHi) is regulated mainly by Na+/H+ and HCO3-/Cl- antiports through the cell membrane, and amiloride acts on the former, DIDS on the latter to lower pHi. Bicarbonates 57-62 glucose-6-phosphate isomerase Homo sapiens 18-21 9821105-3 1998 Intracellular pH (pHi) is regulated mainly by Na+/H+ and HCO3-/Cl- antiports through the cell membrane, and amiloride acts on the former, DIDS on the latter to lower pHi. Amiloride 108-117 glucose-6-phosphate isomerase Homo sapiens 18-21 9821105-3 1998 Intracellular pH (pHi) is regulated mainly by Na+/H+ and HCO3-/Cl- antiports through the cell membrane, and amiloride acts on the former, DIDS on the latter to lower pHi. Amiloride 108-117 glucose-6-phosphate isomerase Homo sapiens 166-169 9821105-3 1998 Intracellular pH (pHi) is regulated mainly by Na+/H+ and HCO3-/Cl- antiports through the cell membrane, and amiloride acts on the former, DIDS on the latter to lower pHi. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 138-142 glucose-6-phosphate isomerase Homo sapiens 18-21 9821105-3 1998 Intracellular pH (pHi) is regulated mainly by Na+/H+ and HCO3-/Cl- antiports through the cell membrane, and amiloride acts on the former, DIDS on the latter to lower pHi. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 138-142 glucose-6-phosphate isomerase Homo sapiens 166-169 9821105-8 1998 Fluorophotometric measurement of pHi was employed using the pH sensitive dye, bis(carboxyethyl)carboxyfluorescein, which is trapped in viable cells. bis(carboxyethyl)carboxyfluorescein 78-113 glucose-6-phosphate isomerase Homo sapiens 33-36 9821105-11 1998 The pHi of the cells heated in the presence of amiloride was decreased to 6.83. Amiloride 47-56 glucose-6-phosphate isomerase Homo sapiens 4-7 9821105-12 1998 The pHi was further lowered to 6.67 by the treatment with amiloride in combination with DIDS for 2 hours. Amiloride 58-67 glucose-6-phosphate isomerase Homo sapiens 4-7 9821105-12 1998 The pHi was further lowered to 6.67 by the treatment with amiloride in combination with DIDS for 2 hours. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 88-92 glucose-6-phosphate isomerase Homo sapiens 4-7 9688606-3 1998 However, replacement of external Cl- with gluconate caused an H2DIDS-inhibitable (100 microM) increase in the pHi of HCMV-infected cells but not in mock-infected cells. dihydro-DIDS 62-68 glucose-6-phosphate isomerase Homo sapiens 110-113 9688606-4 1998 Continuous exposure to hyperosmotic external media containing CO2/HCO-3 caused the pHi of both cell types to increase. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 62-65 glucose-6-phosphate isomerase Homo sapiens 83-86 9688606-4 1998 Continuous exposure to hyperosmotic external media containing CO2/HCO-3 caused the pHi of both cell types to increase. Bicarbonates 66-71 glucose-6-phosphate isomerase Homo sapiens 83-86 9688606-7 1998 This pHi recovery phase was completely blocked by 100 microM H2DIDS. dihydro-DIDS 61-67 glucose-6-phosphate isomerase Homo sapiens 5-8 9688606-10 1998 In mock-infected cells, H2DIDS-sensitive, net Cl- efflux decreased as pHi increased, whereas for HCMV-infected cells, efflux increased as pHi increased. dihydro-DIDS 24-30 glucose-6-phosphate isomerase Homo sapiens 70-73 9688606-10 1998 In mock-infected cells, H2DIDS-sensitive, net Cl- efflux decreased as pHi increased, whereas for HCMV-infected cells, efflux increased as pHi increased. dihydro-DIDS 24-30 glucose-6-phosphate isomerase Homo sapiens 138-141 9639537-0 1998 GPI anchor biosynthesis in yeast: phosphoethanolamine is attached to the alpha1,4-linked mannose of the complete precursor glycophospholipid. phosphorylethanolamine 34-53 glucose-6-phosphate isomerase Homo sapiens 0-3 9639537-0 1998 GPI anchor biosynthesis in yeast: phosphoethanolamine is attached to the alpha1,4-linked mannose of the complete precursor glycophospholipid. alpha1,4-linked mannose 73-96 glucose-6-phosphate isomerase Homo sapiens 0-3 9639537-0 1998 GPI anchor biosynthesis in yeast: phosphoethanolamine is attached to the alpha1,4-linked mannose of the complete precursor glycophospholipid. glycophospholipid 123-140 glucose-6-phosphate isomerase Homo sapiens 0-3 9639537-1 1998 Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. Carbohydrates 32-44 glucose-6-phosphate isomerase Homo sapiens 21-24 9639537-1 1998 Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. Carbohydrates 32-44 glucose-6-phosphate isomerase Homo sapiens 195-198 9639537-1 1998 Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. Acetylglucosamine 73-92 glucose-6-phosphate isomerase Homo sapiens 21-24 9639537-1 1998 Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. Mannose 100-108 glucose-6-phosphate isomerase Homo sapiens 21-24 9639537-1 1998 Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. phosphorylethanolamine 114-133 glucose-6-phosphate isomerase Homo sapiens 21-24 9639537-1 1998 Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. etn-p 135-140 glucose-6-phosphate isomerase Homo sapiens 21-24 9639537-1 1998 Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. etn-p 135-140 glucose-6-phosphate isomerase Homo sapiens 195-198 9639537-1 1998 Cells synthesize the GPI anchor carbohydrate core by successively adding N-acetylglucosamine, three mannoses, and phosphoethanolamine (EtN-P) onto phosphatidylinositol, thus forming the complete GPI precursor lipid which is then added to proteins. Phosphatidylinositols 147-167 glucose-6-phosphate isomerase Homo sapiens 21-24 9639537-2 1998 Previously, we isolated a GPI deficient yeast mutant accumulating a GPI intermediate containing only two mannoses, suggesting that it has difficulty in adding the third, alpha1,2-linked Man of GPI anchors. Mannose 105-113 glucose-6-phosphate isomerase Homo sapiens 26-29 9639537-2 1998 Previously, we isolated a GPI deficient yeast mutant accumulating a GPI intermediate containing only two mannoses, suggesting that it has difficulty in adding the third, alpha1,2-linked Man of GPI anchors. Mannose 105-113 glucose-6-phosphate isomerase Homo sapiens 68-71 9639537-2 1998 Previously, we isolated a GPI deficient yeast mutant accumulating a GPI intermediate containing only two mannoses, suggesting that it has difficulty in adding the third, alpha1,2-linked Man of GPI anchors. Mannose 105-113 glucose-6-phosphate isomerase Homo sapiens 68-71 9639537-7 1998 Further analysis of the GPI intermediate accumulating in gpi10 shows it to have the structure Manalpha1-6(EtN-P-)Manalpha1-4GlcNalpha1-6(acyl) Inositol-P-lipid. etn-p 106-111 glucose-6-phosphate isomerase Homo sapiens 24-27 9639537-7 1998 Further analysis of the GPI intermediate accumulating in gpi10 shows it to have the structure Manalpha1-6(EtN-P-)Manalpha1-4GlcNalpha1-6(acyl) Inositol-P-lipid. -4glcnalpha1-6(acyl) inositol-p-lipid 122-159 glucose-6-phosphate isomerase Homo sapiens 24-27 9639537-8 1998 The presence of EtN-P on the alpha1,4-linked Man of GPI anchors is typical of mammalian and a few other organisms but had not been observed in yeast GPI proteins. etn-p 16-21 glucose-6-phosphate isomerase Homo sapiens 52-55 9639537-9 1998 This additional EtN-P is not only found in the abnormal GPI intermediate of gpi10-1 but is equally present on the complete GPI precursor lipid of wild type cells. etn-p 16-21 glucose-6-phosphate isomerase Homo sapiens 56-59 9639537-9 1998 This additional EtN-P is not only found in the abnormal GPI intermediate of gpi10-1 but is equally present on the complete GPI precursor lipid of wild type cells. etn-p 16-21 glucose-6-phosphate isomerase Homo sapiens 123-126 9689466-4 1998 Coinciding with this W-induced biphasic shift of pHi a biphasic alteration of spontaneous bioelectric activity was recorded: as a rule, an up to 30 min lasting increase (excitatory phase) preceded a typical sustained suppression (inhibitory phase). Tungsten 21-22 glucose-6-phosphate isomerase Homo sapiens 49-52 9696525-8 1998 RESULTS: PHi was higher in the mannitol group than in the glycerol and saline groups (P<0.05) 2 h after reperfusion. Mannitol 31-39 glucose-6-phosphate isomerase Homo sapiens 9-12 9696525-8 1998 RESULTS: PHi was higher in the mannitol group than in the glycerol and saline groups (P<0.05) 2 h after reperfusion. Glycerol 58-66 glucose-6-phosphate isomerase Homo sapiens 9-12 9696525-11 1998 CONCLUSIONS: The mannitol group had improved pHi higher than the glycerol group 2 h after reperfusion (P<0.05), while the glycerol group had improved beta-ATP/Pi ratio higher than the mannitol group 6 h after reperfusion (P<0.05). Mannitol 17-25 glucose-6-phosphate isomerase Homo sapiens 45-48 9689002-8 1998 Reducing bath HCO-3 concentration from 10 to 2 mM at constant CO2 (pH 6.8) depolarized V1 and V2, decreased pHi, and lowered aNai. Bicarbonates 14-19 glucose-6-phosphate isomerase Homo sapiens 108-111 9689002-10 1998 In the presence of NE, reducing bath [HCO-3] caused a smaller depolarizations of V1 and V2, and the rate of pHi decrease was significantly reduced. Bicarbonates 38-43 glucose-6-phosphate isomerase Homo sapiens 108-111 9694686-14 1998 This higher pHi might account at least partially for the increased monoclonal antibody production observed at hyperosmolality and coincided furthermore with a faster glutamine consumption rate. Glutamine 166-175 glucose-6-phosphate isomerase Homo sapiens 12-15 10838290-14 1998 Women smokers taking 35microg EE(2) oral contraceptive had thrombophilic changes in PGI/TXA metabolite ratio. txa 88-91 glucose-6-phosphate isomerase Homo sapiens 84-87 9684362-1 1998 Previous work has shown that stomatal opening induced by indole-3-acetic acid (IAA) in epidermal strips of the orchid Paphiopedilum tonsum L. is preceded by a reduction in cytoplasmic pH (pHi) of the guard cells. indoleacetic acid 79-82 glucose-6-phosphate isomerase Homo sapiens 188-191 9696974-8 1998 Measured pHi correlated positively with mixed venous O2 tension (r = 0.21). Oxygen 53-55 glucose-6-phosphate isomerase Homo sapiens 9-12 9696974-9 1998 There were significant negative correlations between measured pHi and both oxygen delivery (r = -0.25) and oxygen consumption (r = 0.28). Oxygen 75-81 glucose-6-phosphate isomerase Homo sapiens 62-65 9696974-9 1998 There were significant negative correlations between measured pHi and both oxygen delivery (r = -0.25) and oxygen consumption (r = 0.28). Oxygen 107-113 glucose-6-phosphate isomerase Homo sapiens 62-65 9696974-12 1998 Arterial blood pressure and mixed venous oxygen saturation correlated better with measured pHi than with other indices of perfusion. Oxygen 41-47 glucose-6-phosphate isomerase Homo sapiens 91-94 9686769-1 1998 We report three patients with bilateral GPi stimulation for stage 4 Parkinson"s disease (PD) with severe levodopa-induced dyskinesias (LID). Levodopa 105-113 glucose-6-phosphate isomerase Homo sapiens 40-43 9608005-1 1998 In nonperfused proximal tubules isolated from chicken long-looped mammalian-type nephrons, intracellular pH (pHi), measured with the pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein, was approximately 7.3 under control conditions (HEPES-buffered medium with pH 7.4 at 37 degrees C) and was reduced to approximately 7.0 in response to NH4Cl pulse. 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein 162-211 glucose-6-phosphate isomerase Homo sapiens 109-112 9608005-2 1998 The rate of recovery of pHi from this level to the resting level was 1) significantly reduced by the removal of Na+ from the bath, 2) significantly increased by the removal of Cl- from the bath, 3) unchanged by the removal of both Na+ and Cl- from the bath, 4) significantly reduced by the addition of either ethylisopropylamiloride or DIDS to the bath, 5) significantly increased by a high bath K+ concentration, and 6) unchanged by the addition of Ba2+ to the bath. ethylisopropylamiloride 309-332 glucose-6-phosphate isomerase Homo sapiens 24-27 9608005-2 1998 The rate of recovery of pHi from this level to the resting level was 1) significantly reduced by the removal of Na+ from the bath, 2) significantly increased by the removal of Cl- from the bath, 3) unchanged by the removal of both Na+ and Cl- from the bath, 4) significantly reduced by the addition of either ethylisopropylamiloride or DIDS to the bath, 5) significantly increased by a high bath K+ concentration, and 6) unchanged by the addition of Ba2+ to the bath. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 336-340 glucose-6-phosphate isomerase Homo sapiens 24-27 9608005-2 1998 The rate of recovery of pHi from this level to the resting level was 1) significantly reduced by the removal of Na+ from the bath, 2) significantly increased by the removal of Cl- from the bath, 3) unchanged by the removal of both Na+ and Cl- from the bath, 4) significantly reduced by the addition of either ethylisopropylamiloride or DIDS to the bath, 5) significantly increased by a high bath K+ concentration, and 6) unchanged by the addition of Ba2+ to the bath. N-methyl-valyl-amiclenomycin 450-454 glucose-6-phosphate isomerase Homo sapiens 24-27 9576482-9 1998 In agreement with this, heat-shocked cells demonstrated increased activity of an HCO3(-)-independent/DIDS-sensitive pHi down-regulator, postulated to be a Cl-/HCO3- exchange. Bicarbonates 81-86 glucose-6-phosphate isomerase Homo sapiens 116-119 9841500-3 1998 We measured intracellular pH (pHi) in single intercalated cells of in vitro microperfused CCD using the fluorescent, pH-sensitive dye, 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein 135-184 glucose-6-phosphate isomerase Homo sapiens 30-33 9841500-5 1998 Adding extracellular CO2/HCO3 decreased B cell pHi while simultaneously increasing Cl/base exchange activity. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 21-24 glucose-6-phosphate isomerase Homo sapiens 47-50 9841500-5 1998 Adding extracellular CO2/HCO3 decreased B cell pHi while simultaneously increasing Cl/base exchange activity. Bicarbonates 25-29 glucose-6-phosphate isomerase Homo sapiens 47-50 9576482-9 1998 In agreement with this, heat-shocked cells demonstrated increased activity of an HCO3(-)-independent/DIDS-sensitive pHi down-regulator, postulated to be a Cl-/HCO3- exchange. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 101-105 glucose-6-phosphate isomerase Homo sapiens 116-119 9576482-9 1998 In agreement with this, heat-shocked cells demonstrated increased activity of an HCO3(-)-independent/DIDS-sensitive pHi down-regulator, postulated to be a Cl-/HCO3- exchange. Bicarbonates 81-85 glucose-6-phosphate isomerase Homo sapiens 116-119 9573560-1 1998 Primary hyperoxaluria type I (PH I) is a congenital error of metabolism that can be manifested by an increased oxalate production, and ultimately result in kidney failure. Oxalates 111-118 glucose-6-phosphate isomerase Homo sapiens 0-28 9569248-5 1998 Monocarboxylates but not dicarboxylates induced a transient acidification of pHi which was inhibited by 5 mM alpha-cyano-4-hydroxycinnamate (CHC) but not by 1 microM DIDS or 500 microM pCMBS. alpha-cyano-4-hydroxycinnamate 109-139 glucose-6-phosphate isomerase Homo sapiens 77-80 9569248-5 1998 Monocarboxylates but not dicarboxylates induced a transient acidification of pHi which was inhibited by 5 mM alpha-cyano-4-hydroxycinnamate (CHC) but not by 1 microM DIDS or 500 microM pCMBS. 4-Chloromercuribenzenesulfonate 185-190 glucose-6-phosphate isomerase Homo sapiens 77-80 9590311-9 1998 Reflectance spectrophotometry disclosed that patients with low gastric pHi had also a significantly (p < .05) lower hemoglobin content index (61 +/- 4 arbitrary units) than patients with normal pHi (81 +/- 3 arbitrary units), whereas oxygen saturation index was similar for both groups. Oxygen 237-243 glucose-6-phosphate isomerase Homo sapiens 71-74 9573560-1 1998 Primary hyperoxaluria type I (PH I) is a congenital error of metabolism that can be manifested by an increased oxalate production, and ultimately result in kidney failure. Oxalates 111-118 glucose-6-phosphate isomerase Homo sapiens 30-34 9573560-2 1998 After a combined liver/kidney transplantation, children with PH I have persistent excretion of oxalate that causes crystal formation in the urinary tract, and could result in systemic oxalosis and eventual graft failure. Oxalates 95-102 glucose-6-phosphate isomerase Homo sapiens 61-65 9573560-5 1998 We found that crystalluria with the OCV measurement is non-invasive, easily performed, and gives feedback on the efficacy of PH I therapy within one hour. n6-[(1r)-2-{[(1r)-1-carboxy-2-methylpropyl]oxy}-1-(mercaptomethyl)-2-oxoethyl]-6-oxo-d-lysine 36-39 glucose-6-phosphate isomerase Homo sapiens 125-129 9644846-5 1998 More findings, demonstrate that the aPL are in fact anti-b2-GP I antibodies directed against a epitope which is expressed when b2-GP I is bound to anionic phospholipid or another suitable surface. Phospholipids 155-167 glucose-6-phosphate isomerase Homo sapiens 60-64 9524136-8 1998 This kinetic effect of extracellular NH4+ can be accounted for by an increase in intracellular pH (pHi), since raising intracellular pH above 8 reduced the extent of inactivation. Ammonium Compounds 37-41 glucose-6-phosphate isomerase Homo sapiens 99-102 9530271-3 1998 Intracellular pH (pHi) was determined by the use of the fluorescent pH-sensitive dye, 2",7"-bis(2- carboxyethyl)-5(6)-carboxyfluorescein (BCECF). 2",7"-bis(2- carboxyethyl)-5(6)-carboxyfluorescein 86-136 glucose-6-phosphate isomerase Homo sapiens 18-21 9616041-2 1998 The GST-GPI fusion protein showed affinities for the substrates glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) similar to those of the native enzyme purified from human red blood cells (RBC). Glucose-6-Phosphate 64-83 glucose-6-phosphate isomerase Homo sapiens 8-11 9616041-2 1998 The GST-GPI fusion protein showed affinities for the substrates glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) similar to those of the native enzyme purified from human red blood cells (RBC). Glucose-6-Phosphate 85-88 glucose-6-phosphate isomerase Homo sapiens 8-11 9616041-2 1998 The GST-GPI fusion protein showed affinities for the substrates glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) similar to those of the native enzyme purified from human red blood cells (RBC). fructose-6-phosphate 94-114 glucose-6-phosphate isomerase Homo sapiens 8-11 9616041-2 1998 The GST-GPI fusion protein showed affinities for the substrates glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) similar to those of the native enzyme purified from human red blood cells (RBC). fructose-6-phosphate 116-119 glucose-6-phosphate isomerase Homo sapiens 8-11 9616041-4 1998 Although each mutation caused reduced thermal stability, an amino acid substitution Thr-5-->Ile (T5I) exhibited marked thermal instability, suggesting that the amino-terminal of GPI is important for enzymatic stability. Threonine 84-87 glucose-6-phosphate isomerase Homo sapiens 181-184 9616041-4 1998 Although each mutation caused reduced thermal stability, an amino acid substitution Thr-5-->Ile (T5I) exhibited marked thermal instability, suggesting that the amino-terminal of GPI is important for enzymatic stability. Isoleucine 95-98 glucose-6-phosphate isomerase Homo sapiens 181-184 9530271-3 1998 Intracellular pH (pHi) was determined by the use of the fluorescent pH-sensitive dye, 2",7"-bis(2- carboxyethyl)-5(6)-carboxyfluorescein (BCECF). bcecf 138-143 glucose-6-phosphate isomerase Homo sapiens 18-21 9589327-1 1998 Cells which have been adapted to growth at low extracellular pH (pHe) typically develop both an upregulation of steady state intracellular pH (pHi) and an ability to develop thermotolerance to 42 degrees C hyperthermia. Phenylalanine 65-68 glucose-6-phosphate isomerase Homo sapiens 143-146 9589327-4 1998 A better correlation with adaptation to low pHe (as defined by hyperthermia) was found with changes in proton extrusion and the rate of pHi recovery after cytosolic acidification. Phenylalanine 44-47 glucose-6-phosphate isomerase Homo sapiens 136-139 10374640-6 1998 Depletion of intracellular Ca2+ store with ionomycin in the presence of EGTA, no increment in pHi was observed, the basal value of pHi was even more acidic, this response of pHi to thrombin was rehabilitated after refilling of intracellular Ca2+ store with extracellular Ca2+ 1 mmol.L-1. Ionomycin 43-52 glucose-6-phosphate isomerase Homo sapiens 131-134 10374640-6 1998 Depletion of intracellular Ca2+ store with ionomycin in the presence of EGTA, no increment in pHi was observed, the basal value of pHi was even more acidic, this response of pHi to thrombin was rehabilitated after refilling of intracellular Ca2+ store with extracellular Ca2+ 1 mmol.L-1. Ionomycin 43-52 glucose-6-phosphate isomerase Homo sapiens 131-134 9493127-1 1998 We used 31P NMR to investigate the temperature-dependence of intracellular pH (pHi) in isolated frog skeletal muscles. ET bromodomain inhibitor 8-11 glucose-6-phosphate isomerase Homo sapiens 79-82 9493127-5 1998 Acid loading followed by washout resulted in an amiloride-sensitive return to the (temperature dependent) basal pHi. Amiloride 48-57 glucose-6-phosphate isomerase Homo sapiens 112-115 9928909-1 1998 The authors have previously reported decreased intracellular pH (pHi) in the frontal lobes in euthymic bipolar patients treated with lithium using 31P-MRS. White matter hyperintensity (WMHI) is frequently seen in bipolar disorder. Lithium 133-140 glucose-6-phosphate isomerase Homo sapiens 65-68 9503217-7 1998 Ranitidine significantly increased gastric juice pH, but did not affect PCO2i or pHi; pHi was 7.34 +/- 0.14 before ranitidine, and 7.30 +/- 0.12, 7.31 +/- 0.11, 7.31 +/- 0.14 and 7.31 +/- 0.12-2, 4, 6 and 8 h, respectively, after ranitidine administration (p = 0.55). Ranitidine 0-10 glucose-6-phosphate isomerase Homo sapiens 49-51 9488250-11 1998 In examining the determinants of pHi, the intramucosal-arterial PCO2 difference was improved after enalaprilat administration (27 +/- 6 to 17 +/- 3 mmHg, p = .04) while no difference was observed in arterial bicarbonate (19.5 +/- .7 to 19.7 +/- .8, p = .90). pco2 64-68 glucose-6-phosphate isomerase Homo sapiens 33-36 9488250-11 1998 In examining the determinants of pHi, the intramucosal-arterial PCO2 difference was improved after enalaprilat administration (27 +/- 6 to 17 +/- 3 mmHg, p = .04) while no difference was observed in arterial bicarbonate (19.5 +/- .7 to 19.7 +/- .8, p = .90). Enalaprilat 99-110 glucose-6-phosphate isomerase Homo sapiens 33-36 9488250-11 1998 In examining the determinants of pHi, the intramucosal-arterial PCO2 difference was improved after enalaprilat administration (27 +/- 6 to 17 +/- 3 mmHg, p = .04) while no difference was observed in arterial bicarbonate (19.5 +/- .7 to 19.7 +/- .8, p = .90). Bicarbonates 208-219 glucose-6-phosphate isomerase Homo sapiens 33-36 9488250-12 1998 Additionally, the change in pHi observed with enalaprilat correlated with predrug intramucosal-arterial PCO2 difference (r = .74, r2 = .55, p = .0005). Enalaprilat 46-57 glucose-6-phosphate isomerase Homo sapiens 28-31 9488250-12 1998 Additionally, the change in pHi observed with enalaprilat correlated with predrug intramucosal-arterial PCO2 difference (r = .74, r2 = .55, p = .0005). pco2 104-108 glucose-6-phosphate isomerase Homo sapiens 28-31 9503217-0 1998 Effect of ranitidine on gastric intramucosal pH in critically ill patients. Ranitidine 10-20 glucose-6-phosphate isomerase Homo sapiens 45-47 9503217-1 1998 OBJECTIVE: To determine whether ranitidine a) increases the values of gastric intramucosal pH (pHi) in critically ill patients, as determined by tonometry; b) reduces the variability of these measurements. ranitidine a 32-44 glucose-6-phosphate isomerase Homo sapiens 91-93 9503217-1 1998 OBJECTIVE: To determine whether ranitidine a) increases the values of gastric intramucosal pH (pHi) in critically ill patients, as determined by tonometry; b) reduces the variability of these measurements. ranitidine a 32-44 glucose-6-phosphate isomerase Homo sapiens 95-98 9928909-5 1998 These results suggest that decrease of pHi is not an effect of lithium but is instead related to the pathophysiology of illness. Lithium 63-70 glucose-6-phosphate isomerase Homo sapiens 39-42 9616770-2 1997 We propose that DA produces this net effect by a direct influence on the striato-GPI and striato-SNPR neurons, as well as indirectly via the striato-GPE-STN-GPI/SNPR circuit. amsonic acid 16-18 glucose-6-phosphate isomerase Homo sapiens 157-160 9549740-4 1998 After an equilibration period of 30 min, gastric pHi was calculated by applying the Henderson-Hasselbalch equation on the PCO2 obtained with the tonometer and the bicarbonate from the arterial blood gas analysis. Bicarbonates 163-174 glucose-6-phosphate isomerase Homo sapiens 49-52 9616770-1 1997 Taken together with electrophysiological data, these results suggest that in states of DA deficiency, systemically administered L-dopa or DA agonist drugs inhibit cell firing in the major output nuclei of the basal ganglia (GPI and SNPR). Levodopa 128-134 glucose-6-phosphate isomerase Homo sapiens 224-227 9616770-3 1997 The RCGU data suggest that DA activates the direct pathway by stimulating D1 receptor-bearing striatal GABAergic neurons projecting to GPI and SNPR. amsonic acid 27-29 glucose-6-phosphate isomerase Homo sapiens 135-138 9616770-1 1997 Taken together with electrophysiological data, these results suggest that in states of DA deficiency, systemically administered L-dopa or DA agonist drugs inhibit cell firing in the major output nuclei of the basal ganglia (GPI and SNPR). amsonic acid 138-140 glucose-6-phosphate isomerase Homo sapiens 224-227 9616770-8 1997 According to this scheme, DA exerts complementary actions via both direct and indirect anatomical pathways to decrease tonic firing rates of intrinsic neurons in the major output nuclei of the basal ganglia (i.e., GPI and SNPR). amsonic acid 26-28 glucose-6-phosphate isomerase Homo sapiens 214-217 9616770-2 1997 We propose that DA produces this net effect by a direct influence on the striato-GPI and striato-SNPR neurons, as well as indirectly via the striato-GPE-STN-GPI/SNPR circuit. amsonic acid 16-18 glucose-6-phosphate isomerase Homo sapiens 81-84 9435694-2 1997 Among the multiple transport processes that regulate VSMC pHi, Na(+)-independent Cl-/HCO3- exchange is the major process that acidifies VSMCs in response to an alkaline load. Bicarbonates 85-89 glucose-6-phosphate isomerase Homo sapiens 58-61 9429665-2 1997 The three transport pathways of importance for the control of pHi are a sodium-coupled bicarbonate transport, a Na,H-exchanger and a Cl,HCO3- exchange. Sodium 72-78 glucose-6-phosphate isomerase Homo sapiens 62-65 9429665-2 1997 The three transport pathways of importance for the control of pHi are a sodium-coupled bicarbonate transport, a Na,H-exchanger and a Cl,HCO3- exchange. Bicarbonates 87-98 glucose-6-phosphate isomerase Homo sapiens 62-65 9429665-2 1997 The three transport pathways of importance for the control of pHi are a sodium-coupled bicarbonate transport, a Na,H-exchanger and a Cl,HCO3- exchange. Bicarbonates 136-140 glucose-6-phosphate isomerase Homo sapiens 62-65 9447934-3 1997 The type of transporter was identified in functional studies by monitoring uptake of monocarboxylates into SMC through measurement of the cytosolic pH (pHi) with the pH-sensitive dye 2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). monocarboxylates 85-101 glucose-6-phosphate isomerase Homo sapiens 152-155 9447934-3 1997 The type of transporter was identified in functional studies by monitoring uptake of monocarboxylates into SMC through measurement of the cytosolic pH (pHi) with the pH-sensitive dye 2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). 2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein 183-233 glucose-6-phosphate isomerase Homo sapiens 152-155 9447934-3 1997 The type of transporter was identified in functional studies by monitoring uptake of monocarboxylates into SMC through measurement of the cytosolic pH (pHi) with the pH-sensitive dye 2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). bcecf 235-240 glucose-6-phosphate isomerase Homo sapiens 152-155 9447934-6 1997 The monocarboxylates acetate and pyruvate (each 20 mM) induced an acidification of pHi. Acetates 21-28 glucose-6-phosphate isomerase Homo sapiens 83-86 9447934-6 1997 The monocarboxylates acetate and pyruvate (each 20 mM) induced an acidification of pHi. Pyruvic Acid 33-41 glucose-6-phosphate isomerase Homo sapiens 83-86 9407610-2 1997 We discuss the role of intracellular pH (pHi) in central respiratory responses to CO2 and describe a variety of patterns of pHi regulation in chemosensory areas. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 82-85 glucose-6-phosphate isomerase Homo sapiens 41-44 9441471-7 1997 It has been customary to calculate so-called interstitial pH (pHi) by incorporating the measured pCO2 in the tonometer and the HCO3- in an arterial blood gas in the Henderson-Hasselbalch equation. pco2 97-101 glucose-6-phosphate isomerase Homo sapiens 62-65 9441471-7 1997 It has been customary to calculate so-called interstitial pH (pHi) by incorporating the measured pCO2 in the tonometer and the HCO3- in an arterial blood gas in the Henderson-Hasselbalch equation. Bicarbonates 127-131 glucose-6-phosphate isomerase Homo sapiens 62-65 9655153-0 1997 Effect of bicarbonate-based dialysis solutions on intracellular pH (pHi) and TNFalpha production by peritoneal macrophages. Bicarbonates 10-21 glucose-6-phosphate isomerase Homo sapiens 68-71 9655153-8 1997 The pHi was measured by spectrofluorometry in BCECF-loaded PMphi exposed to different dialysis solutions or Hank"s balanced salt solution. hank"s balanced salt solution 108-137 glucose-6-phosphate isomerase Homo sapiens 4-7 9655153-13 1997 Following an initial drop, pHi stabilized after 4 minutes at levels of 6.96 and 6.8 after incubation in TB and TBL, respectively. Terbium 104-106 glucose-6-phosphate isomerase Homo sapiens 27-30 9655153-13 1997 Following an initial drop, pHi stabilized after 4 minutes at levels of 6.96 and 6.8 after incubation in TB and TBL, respectively. thalicarpine 111-114 glucose-6-phosphate isomerase Homo sapiens 27-30 9655153-19 1997 CONCLUSIONS: In contrast to Dianeal, both bicarbonate-based solutions caused only a mild drop in pHi of PMphi. Bicarbonates 42-53 glucose-6-phosphate isomerase Homo sapiens 97-100 9407610-3 1997 One pattern, in which pHi retains a fixed relationship to the CO2 stimulus over time, seems well suited to chemoreceptor cells. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 62-65 glucose-6-phosphate isomerase Homo sapiens 22-25 9376463-9 1997 Coupling of the locally derived piCO2 with systemic arterial HCO3- concentration that results in the pHi as the sensitive parameter of the gastrointestinal malperfusion as suggested by Fiddian Green, is not correct. pico2 32-37 glucose-6-phosphate isomerase Homo sapiens 101-104 9338142-2 1997 ATP increased baseline pHi and the rate of acid recovery in BPAEC. Adenosine Triphosphate 0-3 glucose-6-phosphate isomerase Homo sapiens 23-26 9338142-3 1997 This response was inhibited by the amiloride analogue, methyisobutylamiloride, demonstrating that activation of the Na+/H+ antiport was responsible for the increase in baseline pHi and the recovery from acidosis. Amiloride 35-44 glucose-6-phosphate isomerase Homo sapiens 177-180 9338142-3 1997 This response was inhibited by the amiloride analogue, methyisobutylamiloride, demonstrating that activation of the Na+/H+ antiport was responsible for the increase in baseline pHi and the recovery from acidosis. methyisobutylamiloride 55-77 glucose-6-phosphate isomerase Homo sapiens 177-180 9402543-7 1997 The slower recovery of PCr/(Pi+PCr) is likely to be caused by a combination of several factors, including the lower pHi in MHS subjects at the start of recovery (inhibiting ATP production) and excessive sarcoplasmic calcium overload (causing continued enzyme activation and ATP consumption). Adenosine Triphosphate 173-176 glucose-6-phosphate isomerase Homo sapiens 116-119 9314345-6 1997 We also report based on protein sequence analysis that the amino terminal segment (which includes the site D epitope) of GpI allergens from seven different grass species is highly conserved and contains two hydroxyproline residues and an N-linked carbohydrate moiety. Hydroxyproline 207-221 glucose-6-phosphate isomerase Homo sapiens 121-124 9314345-6 1997 We also report based on protein sequence analysis that the amino terminal segment (which includes the site D epitope) of GpI allergens from seven different grass species is highly conserved and contains two hydroxyproline residues and an N-linked carbohydrate moiety. Nitrogen 238-239 glucose-6-phosphate isomerase Homo sapiens 121-124 9314345-6 1997 We also report based on protein sequence analysis that the amino terminal segment (which includes the site D epitope) of GpI allergens from seven different grass species is highly conserved and contains two hydroxyproline residues and an N-linked carbohydrate moiety. Carbohydrates 247-259 glucose-6-phosphate isomerase Homo sapiens 121-124 9277338-2 1997 We found that the resting pHi of two-cell embryos (40-50 h after human chorionic gonadotropin) in HCO3(-)-free M2 was 7.31 +/- 0.01 (n = 172 embryos), which is significantly above the level predicted if H+ is at electrochemical equilibrium. Bicarbonates 98-102 glucose-6-phosphate isomerase Homo sapiens 26-29 9376463-9 1997 Coupling of the locally derived piCO2 with systemic arterial HCO3- concentration that results in the pHi as the sensitive parameter of the gastrointestinal malperfusion as suggested by Fiddian Green, is not correct. Bicarbonates 61-65 glucose-6-phosphate isomerase Homo sapiens 101-104 9288505-8 1997 pHi correlated with arterial pH (r = 0.66), central-peripheral temperature difference (r = -0.36), lactate (r = -0.32) and central venous pressure (r = -0.21). Lactic Acid 99-106 glucose-6-phosphate isomerase Homo sapiens 0-3 9263149-5 1997 In all their tested sera we found antibodies to phospholipid-free beta 2-glycoprotein I (a beta 2-GPI). Phospholipids 48-60 glucose-6-phosphate isomerase Homo sapiens 98-101 9266190-5 1997 The c1039 C-->T substitution, found in the gene of GPI "Zwickau", has been described recently [30] and causes an Arg 347-->Cys substitution close to the putative catalytic site. Arginine 116-119 glucose-6-phosphate isomerase Homo sapiens 54-57 9266190-5 1997 The c1039 C-->T substitution, found in the gene of GPI "Zwickau", has been described recently [30] and causes an Arg 347-->Cys substitution close to the putative catalytic site. Cysteine 129-132 glucose-6-phosphate isomerase Homo sapiens 54-57 9266190-6 1997 The second mutation in this case is a novel c1538 G-->A substitution causing a Trp-->stop mutation at position 513 apparently resulting in premature RNA degradation thus resulting either in a complete lack of protein or a protein which does not show GPI activity. Tryptophan 82-85 glucose-6-phosphate isomerase Homo sapiens 256-259 9378699-4 1997 The GPI anchor precursors are synthesized in the endoplasmic reticulum by sequential addition of sugar and other components to phosphatidylinositol. Sugars 97-102 glucose-6-phosphate isomerase Homo sapiens 4-7 9378699-4 1997 The GPI anchor precursors are synthesized in the endoplasmic reticulum by sequential addition of sugar and other components to phosphatidylinositol. Phosphatidylinositols 127-147 glucose-6-phosphate isomerase Homo sapiens 4-7 9290986-0 1997 Effect of sucralfate on gastric intramucosal pH in critically ill patients. Sucralfate 10-20 glucose-6-phosphate isomerase Homo sapiens 45-47 9211814-4 1997 The magnitudes of the peak and plateau of [Ca2+]i transients induced by carbachol (CCH, 10(-6) mol/l) were greatly enhanced by an acidic pHi and nearly abolished by an alkaline pHi. Carbachol 72-81 glucose-6-phosphate isomerase Homo sapiens 137-140 9211814-4 1997 The magnitudes of the peak and plateau of [Ca2+]i transients induced by carbachol (CCH, 10(-6) mol/l) were greatly enhanced by an acidic pHi and nearly abolished by an alkaline pHi. Carbachol 72-81 glucose-6-phosphate isomerase Homo sapiens 177-180 9211814-4 1997 The magnitudes of the peak and plateau of [Ca2+]i transients induced by carbachol (CCH, 10(-6) mol/l) were greatly enhanced by an acidic pHi and nearly abolished by an alkaline pHi. Carbachol 83-86 glucose-6-phosphate isomerase Homo sapiens 137-140 9211814-4 1997 The magnitudes of the peak and plateau of [Ca2+]i transients induced by carbachol (CCH, 10(-6) mol/l) were greatly enhanced by an acidic pHi and nearly abolished by an alkaline pHi. Carbachol 83-86 glucose-6-phosphate isomerase Homo sapiens 177-180 9211814-6 1997 This effect of pHi was also observed at higher CCH concentrations (10(-4 )and 10(-5) mol/l), at which the inhibitory effect of an alkaline pHi was more pronounced than the stimulatory effect of an acidic pHi. Carbachol 47-50 glucose-6-phosphate isomerase Homo sapiens 15-18 9211814-6 1997 This effect of pHi was also observed at higher CCH concentrations (10(-4 )and 10(-5) mol/l), at which the inhibitory effect of an alkaline pHi was more pronounced than the stimulatory effect of an acidic pHi. Carbachol 47-50 glucose-6-phosphate isomerase Homo sapiens 139-142 9211814-6 1997 This effect of pHi was also observed at higher CCH concentrations (10(-4 )and 10(-5) mol/l), at which the inhibitory effect of an alkaline pHi was more pronounced than the stimulatory effect of an acidic pHi. Carbachol 47-50 glucose-6-phosphate isomerase Homo sapiens 139-142 9211814-7 1997 An acidic pHi shifted the CCH concentration/response curve to the left, whereas an alkaline pHi led to a rightward shift. Carbachol 26-29 glucose-6-phosphate isomerase Homo sapiens 10-13 9211814-10 1997 The InsP3 increase induced by CCH was unaltered at an acidic pHi, but was augmented at an alkaline pHi. Carbachol 30-33 glucose-6-phosphate isomerase Homo sapiens 61-64 9211814-10 1997 The InsP3 increase induced by CCH was unaltered at an acidic pHi, but was augmented at an alkaline pHi. Carbachol 30-33 glucose-6-phosphate isomerase Homo sapiens 99-102 9296360-6 1997 In addition, 4,4"-diisothiocyano-2,2"-disulfonic acid stilbene (DIDS), an inhibitor of anion exchangers, also inhibited NK cell activity, with an IC50 value of 160 microM, and reduced pHi at a similar concentration, although it is not a P-glycoprotein blocker. 4,4"-diisothiocyano-2,2"-disulfonic acid stilbene 13-62 glucose-6-phosphate isomerase Homo sapiens 184-187 9296360-6 1997 In addition, 4,4"-diisothiocyano-2,2"-disulfonic acid stilbene (DIDS), an inhibitor of anion exchangers, also inhibited NK cell activity, with an IC50 value of 160 microM, and reduced pHi at a similar concentration, although it is not a P-glycoprotein blocker. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 64-68 glucose-6-phosphate isomerase Homo sapiens 184-187 9296360-7 1997 Thus, the inhibitory activities of nicardipine, AHC-52, and DIDS toward NK cell activity paralleled their lowering activities of pHi, suggesting the possibility that disregulation of pHi is related to inhibition of NK cell activity. Nicardipine 35-46 glucose-6-phosphate isomerase Homo sapiens 183-186 9300756-2 1997 METHODS AND MATERIALS: The pHi was determined in cells attached to extracellular matrix proteins loaded with the fluorescent indicator dye BCECF at 37 degrees C and during 42 degrees C hyperthermia at an extracellular pH (pHe) of 6.7 or 7.3 in cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 139-144 glucose-6-phosphate isomerase Homo sapiens 27-30 9300756-7 1997 Acute acidification from pHe = 7.3 to pHe = 6.7 at 37 degrees C caused an initial reduction of 0.5-0.8 unit in pHi, but a partial recovery followed during the next 60-90 min. Phenylalanine 25-28 glucose-6-phosphate isomerase Homo sapiens 111-114 9300756-7 1997 Acute acidification from pHe = 7.3 to pHe = 6.7 at 37 degrees C caused an initial reduction of 0.5-0.8 unit in pHi, but a partial recovery followed during the next 60-90 min. Phenylalanine 38-41 glucose-6-phosphate isomerase Homo sapiens 111-114 9290986-1 1997 OBJECTIVE: To determine whether sucralfate administration affects the tonometric measurement of gastric intramucosal pH (pHi). Sucralfate 32-42 glucose-6-phosphate isomerase Homo sapiens 117-119 9290986-1 1997 OBJECTIVE: To determine whether sucralfate administration affects the tonometric measurement of gastric intramucosal pH (pHi). Sucralfate 32-42 glucose-6-phosphate isomerase Homo sapiens 121-124 9227606-1 1997 The aim of our study is to examine the effect of intracellular pH (pHi) on inorganic phosphate (Pi) uptake by a proximal tubular cell line, the opposum kidney (OK) cells. Phosphates 75-94 glucose-6-phosphate isomerase Homo sapiens 67-70 9362562-10 1997 The point mutations found in this patient"s GPI gene support the idea that GPI may have a neurological function in addition to its role in the carbohydrate metabolism; this is due to the presence of a monomeric sequence analogue called neuroleukin (NLK). Carbohydrates 143-155 glucose-6-phosphate isomerase Homo sapiens 44-47 9362562-10 1997 The point mutations found in this patient"s GPI gene support the idea that GPI may have a neurological function in addition to its role in the carbohydrate metabolism; this is due to the presence of a monomeric sequence analogue called neuroleukin (NLK). Carbohydrates 143-155 glucose-6-phosphate isomerase Homo sapiens 75-78 9362562-10 1997 The point mutations found in this patient"s GPI gene support the idea that GPI may have a neurological function in addition to its role in the carbohydrate metabolism; this is due to the presence of a monomeric sequence analogue called neuroleukin (NLK). Carbohydrates 143-155 glucose-6-phosphate isomerase Homo sapiens 236-247 9227606-6 1997 pHi acidification caused a significant decrease in cellular adenosine 3",5"-cyclic monophosphate (cAMP) content, and cAMP-dependent protein kinase inhibitor (H-89) also did not prevent inhibition of cell Pi uptake by pHi acidification. Cyclic AMP 98-102 glucose-6-phosphate isomerase Homo sapiens 0-3 9227606-7 1997 However, pHi acidification stimulated protein kinase C (PKC) activity and inhibition of PKC by PKC inhibitors (bisindolylmaleimide, calphostin C, or staurosporine) or prolonged exposure to phorbol ester abrogated the inhibitory effect of pHi acidification on cell Pi uptake. bisindolylmaleimide 111-130 glucose-6-phosphate isomerase Homo sapiens 9-12 9227606-7 1997 However, pHi acidification stimulated protein kinase C (PKC) activity and inhibition of PKC by PKC inhibitors (bisindolylmaleimide, calphostin C, or staurosporine) or prolonged exposure to phorbol ester abrogated the inhibitory effect of pHi acidification on cell Pi uptake. calphostin C 132-144 glucose-6-phosphate isomerase Homo sapiens 9-12 9227606-7 1997 However, pHi acidification stimulated protein kinase C (PKC) activity and inhibition of PKC by PKC inhibitors (bisindolylmaleimide, calphostin C, or staurosporine) or prolonged exposure to phorbol ester abrogated the inhibitory effect of pHi acidification on cell Pi uptake. Staurosporine 149-162 glucose-6-phosphate isomerase Homo sapiens 9-12 9227606-7 1997 However, pHi acidification stimulated protein kinase C (PKC) activity and inhibition of PKC by PKC inhibitors (bisindolylmaleimide, calphostin C, or staurosporine) or prolonged exposure to phorbol ester abrogated the inhibitory effect of pHi acidification on cell Pi uptake. Phorbol Esters 189-202 glucose-6-phosphate isomerase Homo sapiens 9-12 9160715-3 1997 These experiments tested the hypothesis that loss of sperm cholesterol leads to a rise in the intracellular pH (pH(i)) that makes the sperm responsive to progesterone. Cholesterol 59-70 glucose-6-phosphate isomerase Homo sapiens 112-117 9180272-3 1997 Ca2+ channels were expressed in human embryonic kidney cells (HEK 293) and pHi was increased from a basal level of 7.3 to 8.3 by exposure of cells to NH4Cl (20 mM) or by elevation of extracellular pH to 8.5. Ammonium Chloride 150-155 glucose-6-phosphate isomerase Homo sapiens 75-78 9142009-7 1997 The cGMP analogue 8-bromo-cGMP depressed cell shortening and pHi in the control myocytes but failed to modify cell contraction or pHi in the hypertrophied cells. Cyclic GMP 4-8 glucose-6-phosphate isomerase Homo sapiens 61-64 9142009-7 1997 The cGMP analogue 8-bromo-cGMP depressed cell shortening and pHi in the control myocytes but failed to modify cell contraction or pHi in the hypertrophied cells. 8-bromocyclic GMP 18-30 glucose-6-phosphate isomerase Homo sapiens 61-64 9160715-3 1997 These experiments tested the hypothesis that loss of sperm cholesterol leads to a rise in the intracellular pH (pH(i)) that makes the sperm responsive to progesterone. Progesterone 154-166 glucose-6-phosphate isomerase Homo sapiens 112-117 9160715-4 1997 pH(i) was measured using BCECF (2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein) in freshly ejaculated sperm (T0 sperm) and in sperm incubated in vitro overnight (T24 sperm). bcecf 25-30 glucose-6-phosphate isomerase Homo sapiens 0-5 9160715-4 1997 pH(i) was measured using BCECF (2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein) in freshly ejaculated sperm (T0 sperm) and in sperm incubated in vitro overnight (T24 sperm). 2",7"-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein 32-82 glucose-6-phosphate isomerase Homo sapiens 0-5 9160715-6 1997 Incubating sperm 24 h in medium supplemented with 1 microM cholesterol to prevent loss of sperm cholesterol suppressed the rise of pH(i) (T24C sperm, pH(i) = 6.96 +/- 0.03, n = 4, p = 0.64 compared to T0 sperm). Cholesterol 59-70 glucose-6-phosphate isomerase Homo sapiens 131-136 9160715-6 1997 Incubating sperm 24 h in medium supplemented with 1 microM cholesterol to prevent loss of sperm cholesterol suppressed the rise of pH(i) (T24C sperm, pH(i) = 6.96 +/- 0.03, n = 4, p = 0.64 compared to T0 sperm). Cholesterol 59-70 glucose-6-phosphate isomerase Homo sapiens 150-155 9160715-6 1997 Incubating sperm 24 h in medium supplemented with 1 microM cholesterol to prevent loss of sperm cholesterol suppressed the rise of pH(i) (T24C sperm, pH(i) = 6.96 +/- 0.03, n = 4, p = 0.64 compared to T0 sperm). Cholesterol 96-107 glucose-6-phosphate isomerase Homo sapiens 131-136 9160715-6 1997 Incubating sperm 24 h in medium supplemented with 1 microM cholesterol to prevent loss of sperm cholesterol suppressed the rise of pH(i) (T24C sperm, pH(i) = 6.96 +/- 0.03, n = 4, p = 0.64 compared to T0 sperm). Cholesterol 96-107 glucose-6-phosphate isomerase Homo sapiens 150-155 9160715-12 1997 In summary, pH(i) increases during incubation in vitro in a cholesterol-dependent fashion. Cholesterol 60-71 glucose-6-phosphate isomerase Homo sapiens 12-17 9096607-6 1997 In the absence of HCO3-, pHi recovery from an intracellular acid load (ammonia pre-pulse technique) was Na(+)-dependent and amiloride-inhibitable. Ammonia 71-78 glucose-6-phosphate isomerase Homo sapiens 25-28 9096607-6 1997 In the absence of HCO3-, pHi recovery from an intracellular acid load (ammonia pre-pulse technique) was Na(+)-dependent and amiloride-inhibitable. Amiloride 124-133 glucose-6-phosphate isomerase Homo sapiens 25-28 9096607-10 1997 Acute removal of extracellular Cl induced a pHi alkalinization that was inhibited by DIDS. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 85-89 glucose-6-phosphate isomerase Homo sapiens 44-47 9096607-11 1997 pHi recovery from an intracellular alkaline load (isohydric CO2 changes) was Cl(-)-dependent and DIDS-inhibitable. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 60-63 glucose-6-phosphate isomerase Homo sapiens 0-3 9096607-11 1997 pHi recovery from an intracellular alkaline load (isohydric CO2 changes) was Cl(-)-dependent and DIDS-inhibitable. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 97-101 glucose-6-phosphate isomerase Homo sapiens 0-3 9087585-2 1997 Muscles were loaded with the fluorescent dye 2"-7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester for simultaneous measurement of pHi and contractility. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 45-114 glucose-6-phosphate isomerase Homo sapiens 147-150 9087660-0 1997 Effects of pH on basolateral tetraethylammonium transport in snake renal proximal tubules. Tetraethylammonium 29-47 glucose-6-phosphate isomerase Homo sapiens 11-13 9087585-7 1997 In the presence of 5 microM ethyl isopropyl amiloride (EIPA, to specifically inhibit the Na+/H+ exchanger), the alkalinizing effect of ANG II was changed to a significant decrease in pHi, despite which ANG II still increased contractility by 87 +/- 16% (n = 6). ethylisopropylamiloride 28-53 glucose-6-phosphate isomerase Homo sapiens 183-186 9087660-1 1997 Previous work on snake renal proximal tubules suggested that pH might influence tetraethylammonium (TEA) transport across the basolateral membrane. Tetraethylammonium 80-98 glucose-6-phosphate isomerase Homo sapiens 61-63 9087660-1 1997 Previous work on snake renal proximal tubules suggested that pH might influence tetraethylammonium (TEA) transport across the basolateral membrane. Tetraethylammonium 100-103 glucose-6-phosphate isomerase Homo sapiens 61-63 9087660-2 1997 To examine this more directly, we determined the effects of altering either extracellular pH (pHo) or intracellular pH (pHi) on TEA uptake and efflux across the basolateral membrane of isolated snake renal proximal tubules. Tetraethylammonium 128-131 glucose-6-phosphate isomerase Homo sapiens 120-123 9087660-7 1997 Moreover, there appeared to be an optimal intracellular H+ concentration for entry of TEA into the cells that corresponded to the one found at the physiological pHi of 7.1. Tetraethylammonium 86-89 glucose-6-phosphate isomerase Homo sapiens 161-164 9087585-7 1997 In the presence of 5 microM ethyl isopropyl amiloride (EIPA, to specifically inhibit the Na+/H+ exchanger), the alkalinizing effect of ANG II was changed to a significant decrease in pHi, despite which ANG II still increased contractility by 87 +/- 16% (n = 6). ethylisopropylamiloride 55-59 glucose-6-phosphate isomerase Homo sapiens 183-186 9089403-2 1997 We evaluated pHi-sensitivity of these mutants by measuring the resting pHi in cells placed in an acidic medium (pH 6.0) and pHi-dependence of 5-(N-ethyl-N-isopropyl)amiloride-sensitive 22Na+ uptake. ethylisopropylamiloride 142-174 glucose-6-phosphate isomerase Homo sapiens 13-16 9083230-12 1997 In the norepinephrine-dobutamine group pHi (from 7.30 +/- 0.11 to 7.35 +/- 0.07) and the PCO2 gap (from 10 +/- 3.0 to 4 +/- 2.0 mmHg) were normalized within 6 h (p < 0.01). Norepinephrine 7-21 glucose-6-phosphate isomerase Homo sapiens 39-42 9083230-12 1997 In the norepinephrine-dobutamine group pHi (from 7.30 +/- 0.11 to 7.35 +/- 0.07) and the PCO2 gap (from 10 +/- 3.0 to 4 +/- 2.0 mmHg) were normalized within 6 h (p < 0.01). Dobutamine 22-32 glucose-6-phosphate isomerase Homo sapiens 39-42 9083230-13 1997 The decrease in pHi and the increase in the lactate/pyruvate ratio in the epinephrine group was transient, since it returned to normal within 24 h. CONCLUSIONS: Considering the global hemodynamic effects, epinephrine is as effective as norepinephrine-dobutamine. Epinephrine 74-85 glucose-6-phosphate isomerase Homo sapiens 16-19 9089403-6 1997 Deletion of subdomain I abolished the decrease of pHi-sensitivity induced by cell ATP depletion, indicating that domain I plays a crucial role in this phenomenon. Adenosine Triphosphate 82-85 glucose-6-phosphate isomerase Homo sapiens 50-53 9099966-5 1997 Combined use of the cytostatic drugs with pHi modifiers reduced their cytotoxicity under both physiological and low-pHe conditions. Phenylalanine 116-119 glucose-6-phosphate isomerase Homo sapiens 42-45 14646543-6 1997 The Na+/H+ antiporter inhibitor of 5-(N,N"-dimethyl)-amiloride inhibited the dexamethasone-induced increase in pHi and apoptosis of thymocytes. 5-dimethylamiloride 35-62 glucose-6-phosphate isomerase Homo sapiens 111-114 14646543-6 1997 The Na+/H+ antiporter inhibitor of 5-(N,N"-dimethyl)-amiloride inhibited the dexamethasone-induced increase in pHi and apoptosis of thymocytes. Dexamethasone 77-90 glucose-6-phosphate isomerase Homo sapiens 111-114 8985269-5 1997 First, we determined if fructose inhibited apoptosis by decreasing adenosine triphosphate (ATP) and intracellular pH (pHi). Fructose 24-32 glucose-6-phosphate isomerase Homo sapiens 118-121 8985269-7 1997 Fructose (10 mmol/L) decreased intracellular pH (pHi) by 0.2 U. Fructose 0-8 glucose-6-phosphate isomerase Homo sapiens 49-52 9318556-0 1997 Increased concentrations of haemolymph Mg2+ protect intracellular pH and ATP levels during temperature stress and anoxia in the common shrimp The effects of temperature (0, 5, 10, 15 and 20 &deg;C) and of anoxia (at 5 &deg;C) on extracellular Mg2+ concentration ([Mg2+]e), intracellular pH (pHi) and ATP and lactate levels were investigated in intermoult adults of the common shrimp Crangon crangon. magnesium ion 39-43 glucose-6-phosphate isomerase Homo sapiens 299-302 9318556-4 1997 The influence of high extracellular [Mg2+] on pHi and intracellular ATP and lactate levels under normoxic and anoxic conditions was tested using an incubation medium containing 150&shy;250 mmol l-1 Mg2+. magnesium ion 37-41 glucose-6-phosphate isomerase Homo sapiens 46-49 9318556-6 1997 In addition, the elevation of haemolymph [Mg2+] by incubation in high-[Mg2+] water prevented the drop in pHi and the rise in lactate levels induced by anoxia. magnesium ion 42-46 glucose-6-phosphate isomerase Homo sapiens 105-108 9318556-6 1997 In addition, the elevation of haemolymph [Mg2+] by incubation in high-[Mg2+] water prevented the drop in pHi and the rise in lactate levels induced by anoxia. magnesium ion 71-75 glucose-6-phosphate isomerase Homo sapiens 105-108 9318556-6 1997 In addition, the elevation of haemolymph [Mg2+] by incubation in high-[Mg2+] water prevented the drop in pHi and the rise in lactate levels induced by anoxia. Water 77-82 glucose-6-phosphate isomerase Homo sapiens 105-108 9318556-7 1997 The protective effect of high levels of extracellular Mg2+ did not depend upon the [Ca2+]/[Mg2+] ratio but only on [Mg2+]e. However, experiments with isolated muscle tissues showed no dependence of muscle intracellular pH on [Mg2+]e under both normoxic and anoxic conditions, leading to the conclusion that the protective effect is evoked via a central, possibly anaesthetising, effect of high [Mg2+]e. The dependence of pHi and muscle [ATP] on extracellular [Mg2+] resembles the protective effect of high Mg2+ levels on the post-ischaemic mammalian heart. magnesium ion 54-58 glucose-6-phosphate isomerase Homo sapiens 421-424 8997183-4 1996 In the Langendorff-perfused heart, washout of CO2 brought about by switching perfusion between 25 mM HCO3(-)-5% CO2-buffered solution and nominally HCO3(-)-CO2-free solution caused a transient rise in intracellular pH (pHi) measured by the chemical shift of 2-deoxy-D-glucose 6-phosphate with 31P nuclear magnetic resonance spectroscopy. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 46-49 glucose-6-phosphate isomerase Homo sapiens 219-222 11543590-10 1997 4-Acetamido-4"-isothio-cyanatostilbene-2,2"-disulfonic acid (SITS, 1 mM), a Cl-/HCO3- exchange inhibitor, affected the pHi of hASMCs both in static and flow conditions. 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 0-59 glucose-6-phosphate isomerase Homo sapiens 119-122 11543590-10 1997 4-Acetamido-4"-isothio-cyanatostilbene-2,2"-disulfonic acid (SITS, 1 mM), a Cl-/HCO3- exchange inhibitor, affected the pHi of hASMCs both in static and flow conditions. Bicarbonates 80-84 glucose-6-phosphate isomerase Homo sapiens 119-122 11543590-13 1997 In the case of SMCs, the involvement of pHi changes in nitric oxide production and proliferation regulation highlights further the significance of such studies. Nitric Oxide 55-67 glucose-6-phosphate isomerase Homo sapiens 40-43 8997183-4 1996 In the Langendorff-perfused heart, washout of CO2 brought about by switching perfusion between 25 mM HCO3(-)-5% CO2-buffered solution and nominally HCO3(-)-CO2-free solution caused a transient rise in intracellular pH (pHi) measured by the chemical shift of 2-deoxy-D-glucose 6-phosphate with 31P nuclear magnetic resonance spectroscopy. Bicarbonates 101-108 glucose-6-phosphate isomerase Homo sapiens 219-222 8997183-5 1996 The initial rate of change of pHi, measured over the first 60-75 s of CO2 efflux, was significantly reduced from 0.41 +/- 0.03 pH units/min (n = 9) in control hearts to 0.28 +/- 0.02 pH units/min (n = 5) in the presence of the membrane-permeable CA inhibitor 6-ethoxzolamide (P < 0.05 compared with control) and to 0.22 +/- 0.04 pH units/min (n = 5) in the presence of the membrane-impermeable CA inhibitor CL-11,366 (P < 0.01 compared with control). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 70-73 glucose-6-phosphate isomerase Homo sapiens 30-33 8997183-5 1996 The initial rate of change of pHi, measured over the first 60-75 s of CO2 efflux, was significantly reduced from 0.41 +/- 0.03 pH units/min (n = 9) in control hearts to 0.28 +/- 0.02 pH units/min (n = 5) in the presence of the membrane-permeable CA inhibitor 6-ethoxzolamide (P < 0.05 compared with control) and to 0.22 +/- 0.04 pH units/min (n = 5) in the presence of the membrane-impermeable CA inhibitor CL-11,366 (P < 0.01 compared with control). 6-ethoxzolamide 259-274 glucose-6-phosphate isomerase Homo sapiens 30-33 8997183-5 1996 The initial rate of change of pHi, measured over the first 60-75 s of CO2 efflux, was significantly reduced from 0.41 +/- 0.03 pH units/min (n = 9) in control hearts to 0.28 +/- 0.02 pH units/min (n = 5) in the presence of the membrane-permeable CA inhibitor 6-ethoxzolamide (P < 0.05 compared with control) and to 0.22 +/- 0.04 pH units/min (n = 5) in the presence of the membrane-impermeable CA inhibitor CL-11,366 (P < 0.01 compared with control). cl-11 410-415 glucose-6-phosphate isomerase Homo sapiens 30-33 8997183-7 1996 These results suggest that CA, by facilitating the hydration-dehydration of CO2-H2CO3, alters the relative concentrations of CO2 inside and outside the cells, thus enhancing the rate of CO2 transfer from the intracellular to extracellular compartments, which contributes significantly to pHi recovery after reperfusion of the ischemic myocardium. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 76-79 glucose-6-phosphate isomerase Homo sapiens 288-291 8997183-7 1996 These results suggest that CA, by facilitating the hydration-dehydration of CO2-H2CO3, alters the relative concentrations of CO2 inside and outside the cells, thus enhancing the rate of CO2 transfer from the intracellular to extracellular compartments, which contributes significantly to pHi recovery after reperfusion of the ischemic myocardium. Carbonic Acid 80-85 glucose-6-phosphate isomerase Homo sapiens 288-291 8997183-7 1996 These results suggest that CA, by facilitating the hydration-dehydration of CO2-H2CO3, alters the relative concentrations of CO2 inside and outside the cells, thus enhancing the rate of CO2 transfer from the intracellular to extracellular compartments, which contributes significantly to pHi recovery after reperfusion of the ischemic myocardium. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 125-128 glucose-6-phosphate isomerase Homo sapiens 288-291 8997183-7 1996 These results suggest that CA, by facilitating the hydration-dehydration of CO2-H2CO3, alters the relative concentrations of CO2 inside and outside the cells, thus enhancing the rate of CO2 transfer from the intracellular to extracellular compartments, which contributes significantly to pHi recovery after reperfusion of the ischemic myocardium. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 125-128 glucose-6-phosphate isomerase Homo sapiens 288-291 8931385-2 1996 Nigericin equilibrates intracellular pH (pHi) and extracellular pH (pHe) and is most effective in potentiating PDT damage when cells are in an acidic environment (pH 6.5-6.7). Nigericin 0-9 glucose-6-phosphate isomerase Homo sapiens 41-44 8952952-1 1996 ABA stimulation of outward K+ current (IK,out) in Vicia faba guard cells has been correlated with a rise in cytosolic pH (pHi). alisol B 23-acetate 0-3 glucose-6-phosphate isomerase Homo sapiens 122-125 8952952-4 1996 The stimulatory effect of alkalinizing pHi was voltage insensitive and independent of the two free calcium levels tested, 50 nM and 1 microM. Calcium 99-106 glucose-6-phosphate isomerase Homo sapiens 39-42 8947032-6 1996 Mutational analysis of this domain indicates that proper subcellular localization and cycling of gpI depend on two different determinants, a tyrosine-containing tetrapeptide related to endocytosis sorting signals and a cluster of acidic amino acids containing casein kinase II phosphorylatable residues. Tyrosine 141-149 glucose-6-phosphate isomerase Homo sapiens 97-100 9019738-2 1996 pHi was changed by replacing phosphate buffer by the diffusible buffers CO2/HCO3- or NH3/NH4+ (pH 7.4). Phosphates 29-38 glucose-6-phosphate isomerase Homo sapiens 0-3 9019738-2 1996 pHi was changed by replacing phosphate buffer by the diffusible buffers CO2/HCO3- or NH3/NH4+ (pH 7.4). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 72-75 glucose-6-phosphate isomerase Homo sapiens 0-3 9019738-2 1996 pHi was changed by replacing phosphate buffer by the diffusible buffers CO2/HCO3- or NH3/NH4+ (pH 7.4). Bicarbonates 76-80 glucose-6-phosphate isomerase Homo sapiens 0-3 9019738-2 1996 pHi was changed by replacing phosphate buffer by the diffusible buffers CO2/HCO3- or NH3/NH4+ (pH 7.4). nh3/nh4+ 85-93 glucose-6-phosphate isomerase Homo sapiens 0-3 8987090-15 1996 Similar pH-dependent MMC activity was found when multilayers were pre-treated for 1 hr with 0.5 microgram/ml nigericin, a proton ionophore known to cause the intracellular pH (pHi) to equilibrate with pHe. Nigericin 109-118 glucose-6-phosphate isomerase Homo sapiens 176-179 8973715-6 1996 Although addition of phorbol 12-myristate 13-acetate (TPA) had no effect on pHi in unstimulated conditions, the pHi recovery from an acid load was enhanced by exposure to TPA. Tetradecanoylphorbol Acetate 171-174 glucose-6-phosphate isomerase Homo sapiens 112-115 9120119-9 1996 Intramucosal pH (pHi) calculated from PCO2 air was significantly lower than that calculated from PCO2ss (7.26 +/- 0.23 vs 7.28 +/- 0.24, p = 0.0001). pco2 38-42 glucose-6-phosphate isomerase Homo sapiens 17-20 9120119-12 1996 CONCLUSION: We conclude that intraluminal PCO2 can be accurately determined in postoperative cardiac surgery patients by instilling air into the stomach and analyzing samples of gastric air on a standard blood gas machine, In comparison with saline tonometry, air tonometry consistently yields lower pHi values. pco2 42-46 glucose-6-phosphate isomerase Homo sapiens 300-303 8938899-7 1996 RESULTS: In both villus and crypt cells incubated in Hepes buffer, removal of Na+ or addition of amiloride decreased basal pHi and pHi recovery after intracellular acidification, indicating an Na+/H+ exchanger in both cell types. Amiloride 97-106 glucose-6-phosphate isomerase Homo sapiens 123-126 8938899-7 1996 RESULTS: In both villus and crypt cells incubated in Hepes buffer, removal of Na+ or addition of amiloride decreased basal pHi and pHi recovery after intracellular acidification, indicating an Na+/H+ exchanger in both cell types. Amiloride 97-106 glucose-6-phosphate isomerase Homo sapiens 131-134 8938899-10 1996 Removal of external Cl induced a reversible increase in pHi (inhibited by H2DIDS) in both villus and crypt cells, indicating a Cl-/HCO3- exchanger in both. Bicarbonates 131-135 glucose-6-phosphate isomerase Homo sapiens 56-59 8931385-3 1996 We therefore hypothesized that the ability of nigericin to lower pHi is causally related to its ability to potentiate PDT. Nigericin 46-55 glucose-6-phosphate isomerase Homo sapiens 65-68 8931385-4 1996 To test this, the pHi of A549 cells was reduced using pHe-adjusted growth medium, with or without addition of amiloride and 4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid, inhibitors of the membrane-based exchangers responsible for regulating pHi. Phenylalanine 54-57 glucose-6-phosphate isomerase Homo sapiens 18-21 8931385-5 1996 Using fluorescence ratio imaging, we found that pHi can be equilibrated to within +/- 0.05 pH unit, in the pH range of 6.0-6.8, for up to 1 h after pHe adjustment. Phenylalanine 148-151 glucose-6-phosphate isomerase Homo sapiens 48-51 8931385-9 1996 In contrast, 20 microM nigericin in medium at pHe 6.7 reduces pHi to 6.55, but reduces the surviving fraction at 20 kJ/m2 by nearly 3 logs relative to controls. Nigericin 23-32 glucose-6-phosphate isomerase Homo sapiens 62-65 8822954-7 1996 We also identified GPI Kinki as a compound heterozygote of 1124ACA-->AGA(375Thr-->Arg)/ 1615GAC-->AAC(539Asp-->Asn). Arginine 88-91 glucose-6-phosphate isomerase Homo sapiens 19-22 8914588-1 1996 2",7"-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is frequently used for fluorometric determination of intracellular pH (pHi) and its metabolic changes. 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein 0-55 glucose-6-phosphate isomerase Homo sapiens 135-138 8914588-1 1996 2",7"-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is frequently used for fluorometric determination of intracellular pH (pHi) and its metabolic changes. bcecf 57-62 glucose-6-phosphate isomerase Homo sapiens 135-138 8914588-2 1996 Studies of BCECF-loaded platelets have reported different pHi values in the range of 6.98 to 7.35, despite the use of the same probe. bcecf 11-16 glucose-6-phosphate isomerase Homo sapiens 58-61 8914588-6 1996 When least affected by BCECF, platelet pHi is 7.34. bcecf 23-28 glucose-6-phosphate isomerase Homo sapiens 39-42 8894976-4 1996 Channels were activated by cGMP or cAMP and currents as a function of voltage and cyclic nucleotide concentrations were measured as pH(i) was varied between 7.6 and 5.0. Nucleotides, Cyclic 82-99 glucose-6-phosphate isomerase Homo sapiens 132-137 8894976-8 1996 K(1/2) for cAMP decreased by increasing internal proton concentration whereas maximal currents activated by cAMP increased by lowering pH(i) from 7.6 to 5.7-5.5 and then decreased from pH(i) 5.5 to 5.0. Cyclic AMP 108-112 glucose-6-phosphate isomerase Homo sapiens 135-140 8894976-8 1996 K(1/2) for cAMP decreased by increasing internal proton concentration whereas maximal currents activated by cAMP increased by lowering pH(i) from 7.6 to 5.7-5.5 and then decreased from pH(i) 5.5 to 5.0. Cyclic AMP 108-112 glucose-6-phosphate isomerase Homo sapiens 185-190 9019738-3 1996 CO2/HCO3- buffers at 2,5 or 10% acidified pHi by 0.1, 0.32 and 0.38 pH units, respectively, and increased [Ca2+]i by 8-15 nmol/l. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 0-3 glucose-6-phosphate isomerase Homo sapiens 42-45 9019738-3 1996 CO2/HCO3- buffers at 2,5 or 10% acidified pHi by 0.1, 0.32 and 0.38 pH units, respectively, and increased [Ca2+]i by 8-15 nmol/l. Bicarbonates 4-8 glucose-6-phosphate isomerase Homo sapiens 42-45 9019738-5 1996 Removing the CO2/HCO3- buffer alkalinized pHi by 0.14 (2%), 0.27 (5%), and 0.38 (10%) units and enhanced [Ca2+]i to a peak value of 20, 65, and 143 nmol/l, respectively. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 13-16 glucose-6-phosphate isomerase Homo sapiens 42-45 9019738-5 1996 Removing the CO2/HCO3- buffer alkalinized pHi by 0.14 (2%), 0.27 (5%), and 0.38 (10%) units and enhanced [Ca2+]i to a peak value of 20, 65, and 143 nmol/l, respectively. Bicarbonates 17-21 glucose-6-phosphate isomerase Homo sapiens 42-45 9019738-12 1996 An acid pHi attenuated, and an alkaline pHi enhanced, carbachol- and thapsigargin-induced [Ca2+]i influx. Carbachol 54-63 glucose-6-phosphate isomerase Homo sapiens 8-11 9019738-12 1996 An acid pHi attenuated, and an alkaline pHi enhanced, carbachol- and thapsigargin-induced [Ca2+]i influx. Carbachol 54-63 glucose-6-phosphate isomerase Homo sapiens 40-43 9019738-12 1996 An acid pHi attenuated, and an alkaline pHi enhanced, carbachol- and thapsigargin-induced [Ca2+]i influx. Thapsigargin 69-81 glucose-6-phosphate isomerase Homo sapiens 8-11 9019738-12 1996 An acid pHi attenuated, and an alkaline pHi enhanced, carbachol- and thapsigargin-induced [Ca2+]i influx. Thapsigargin 69-81 glucose-6-phosphate isomerase Homo sapiens 40-43 8862460-2 1996 We hypothesized that this might be due to up-regulation of the alternate pHi regulator, the Na(+)-dependent HCO3-/Cl- exchanger, in the TR-4 cells. Bicarbonates 108-112 glucose-6-phosphate isomerase Homo sapiens 73-76 8862460-7 1996 Results from pHi measurements during heating reflected the clonogenic survival data in that lower pHi levels were associated with the conditions when DIDS was present. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 150-154 glucose-6-phosphate isomerase Homo sapiens 13-16 8862460-7 1996 Results from pHi measurements during heating reflected the clonogenic survival data in that lower pHi levels were associated with the conditions when DIDS was present. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 150-154 glucose-6-phosphate isomerase Homo sapiens 98-101 8794291-8 1996 Exposure of transfected cells to antibodies to the tac ectodomain revealed that the TCN targeting of expressed tac-gpI chimeric proteins occurred as a result of selective retrieval from the plasmalemma. triciribine 84-87 glucose-6-phosphate isomerase Homo sapiens 115-118 8879003-0 1996 Effect of dithiothreitol on quality control of GPI-anchor addition. Dithiothreitol 10-24 glucose-6-phosphate isomerase Homo sapiens 47-50 8917766-1 1996 Rod intracellular pH (pHi) in the intact frog retina was measured fluorometrically with the dye 2",7"-bis(2-carboxyethyl)-5(and-6)-carboxyfluorescein under treatments chosen to affect putative pH-regulating transport mechanisms in the plasma membrane. 2",7"-bis(2-carboxyethyl)-5(and-6)-carboxyfluorescein 96-149 glucose-6-phosphate isomerase Homo sapiens 18-20 8917766-1 1996 Rod intracellular pH (pHi) in the intact frog retina was measured fluorometrically with the dye 2",7"-bis(2-carboxyethyl)-5(and-6)-carboxyfluorescein under treatments chosen to affect putative pH-regulating transport mechanisms in the plasma membrane. 2",7"-bis(2-carboxyethyl)-5(and-6)-carboxyfluorescein 96-149 glucose-6-phosphate isomerase Homo sapiens 22-25 8917766-1 1996 Rod intracellular pH (pHi) in the intact frog retina was measured fluorometrically with the dye 2",7"-bis(2-carboxyethyl)-5(and-6)-carboxyfluorescein under treatments chosen to affect putative pH-regulating transport mechanisms in the plasma membrane. 2",7"-bis(2-carboxyethyl)-5(and-6)-carboxyfluorescein 96-149 glucose-6-phosphate isomerase Homo sapiens 22-24 8917766-4 1996 Bicarbonate-dependent mechanisms were characterized as follows: (1) Replacing half of a 12 mM phosphate buffer by bicarbonate caused a sustained rise of pHi. Bicarbonates 0-11 glucose-6-phosphate isomerase Homo sapiens 153-156 8917766-4 1996 Bicarbonate-dependent mechanisms were characterized as follows: (1) Replacing half of a 12 mM phosphate buffer by bicarbonate caused a sustained rise of pHi. Phosphates 94-103 glucose-6-phosphate isomerase Homo sapiens 153-156 8917766-4 1996 Bicarbonate-dependent mechanisms were characterized as follows: (1) Replacing half of a 12 mM phosphate buffer by bicarbonate caused a sustained rise of pHi. Bicarbonates 114-125 glucose-6-phosphate isomerase Homo sapiens 153-156 8917766-6 1996 (3) Substitution of external Cl- by gluconate (95 mM) caused a rapid pHi rise both in normal Na+ and low-Na+ perfusion. gluconic acid 36-45 glucose-6-phosphate isomerase Homo sapiens 69-72 8917766-9 1996 It is concluded that both Na+/H+ exchange and bicarbonate transport control rod pHi, modulating the light-sensitive current. Bicarbonates 46-57 glucose-6-phosphate isomerase Homo sapiens 80-83 8914181-12 1996 Both in vivo and in vitro lactate, but not bicarbonate, induce a significant drop in pHi (p < 0.05). Lactic Acid 26-33 glucose-6-phosphate isomerase Homo sapiens 85-88 8914181-13 1996 Decreased levels of pHi like those observed in the lactate-incubated RBC were demonstrated to be able to inhibit G-3-PD activity (25 +/- 2%) here used as an indicator of the actual decrease in pH. Lactic Acid 51-58 glucose-6-phosphate isomerase Homo sapiens 20-23 8914181-15 1996 This condition was demonstrated observing a cell energy depletion, which coincides in vitro with an acute EMP impairment; the lactate accumulation together with the consequent lowering of pHi seem to be responsible for this effect, which was not observed when bicarbonate was used instead of lactate. Bicarbonates 260-271 glucose-6-phosphate isomerase Homo sapiens 188-191 8804438-12 1996 We have found that the inhibitors of PLA2, 4-bromophenacyl bromide and manoalide, completely blocked the increase of pH(i) and spreading of neutrophils upon adhesion to solid substrata. 4-bromophenacyl bromide 43-66 glucose-6-phosphate isomerase Homo sapiens 117-122 8804438-12 1996 We have found that the inhibitors of PLA2, 4-bromophenacyl bromide and manoalide, completely blocked the increase of pH(i) and spreading of neutrophils upon adhesion to solid substrata. manoalide 71-80 glucose-6-phosphate isomerase Homo sapiens 117-122 8804438-14 1996 Inhibition of PKC with H-7 or staurosporin increased pH(i). 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine 23-26 glucose-6-phosphate isomerase Homo sapiens 53-58 8804438-14 1996 Inhibition of PKC with H-7 or staurosporin increased pH(i). Staurosporine 30-42 glucose-6-phosphate isomerase Homo sapiens 53-58 8804438-15 1996 PMA, an activator of PKC, dramatically decreased pH(i) but did not impair the spreading of neutrophils. Tetradecanoylphorbol Acetate 0-3 glucose-6-phosphate isomerase Homo sapiens 49-54 8804438-16 1996 The effect of arachidonic acid, a product of PLA2 activity, on neutrophil pH(i) and spreading was similar to that of PMA. Arachidonic Acid 14-30 glucose-6-phosphate isomerase Homo sapiens 74-79 8804438-17 1996 H-7, an inhibitor of PKC, partially blocked the effect of arachidonic acid (AA) on pH(i). 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine 0-3 glucose-6-phosphate isomerase Homo sapiens 83-88 8804438-17 1996 H-7, an inhibitor of PKC, partially blocked the effect of arachidonic acid (AA) on pH(i). Arachidonic Acid 58-74 glucose-6-phosphate isomerase Homo sapiens 83-88 8795298-13 1996 However, the ionomycin effect was still dependent on pHi and was blocked by ryanodine at a higher concentration. Ionomycin 13-22 glucose-6-phosphate isomerase Homo sapiens 53-56 8795298-7 1996 The bradykinin effect was blocked by 5 mumol/L U-73122 (an inhibitor of inositol trisphosphate production); the ionomycin effect was inhibited by increasing intracellular pH (pHi) or treatment with 100 mumol/L ryanodine while the monensin effect was enhanced by increasing pHi, but was not inhibited by ryanodine. Ionomycin 112-121 glucose-6-phosphate isomerase Homo sapiens 175-178 8795298-7 1996 The bradykinin effect was blocked by 5 mumol/L U-73122 (an inhibitor of inositol trisphosphate production); the ionomycin effect was inhibited by increasing intracellular pH (pHi) or treatment with 100 mumol/L ryanodine while the monensin effect was enhanced by increasing pHi, but was not inhibited by ryanodine. Ionomycin 112-121 glucose-6-phosphate isomerase Homo sapiens 273-276 8682873-10 1996 Inhibition of microfilament assembly with cytochalasin D precluded spreading and concomitantly abolished superoxide production and the associated pHi changes, indicating that cytoskeletal reorganization and/or an increase in the number of adherence receptors engaged are required for the responses. Cytochalasin D 42-56 glucose-6-phosphate isomerase Homo sapiens 146-149 8895841-2 1996 In the presence of Na+ at a physiological concentration (147 mM), nigericin (0.5 microM) elevates [Nai] from 20 to 50 mM, increases the pHi, 0.16 pH units, elevates four fold the [Cai] at expense of external Ca2+ and markedly increases (more than five fold) the release of [3H]GABA. Nigericin 66-75 glucose-6-phosphate isomerase Homo sapiens 136-139 8895841-3 1996 In the absence of a Na+ concentration gradient (i.e. when the external Na+ concentration equals the [Nai]), the same concentration (0.5 microM) of nigericin causes the opposite effect on the pHi (acidifies the synaptosomal interior), does not modify the [Nai] and is practically unable to elevate the [Cai] or to increase [3H]GABA release. Nigericin 147-156 glucose-6-phosphate isomerase Homo sapiens 191-194 8760139-3 1996 Treatment of type II cells with 80 nM phorbol 12-myristate 13-acetate (PMA) increased the resting pHi in a time-dependent manner. Tetradecanoylphorbol Acetate 38-69 glucose-6-phosphate isomerase Homo sapiens 98-101 8760139-3 1996 Treatment of type II cells with 80 nM phorbol 12-myristate 13-acetate (PMA) increased the resting pHi in a time-dependent manner. Tetradecanoylphorbol Acetate 71-74 glucose-6-phosphate isomerase Homo sapiens 98-101 8677271-4 1996 pHi in piglet cerebral microvascular endothelial cells in primary culture was monitored using the pH-sensitive fluorescent dye BCECF (2",7"-bis-2-carboxyethyl-5(6)-carboxy-fluorescein acetoxymethyl ester), with dual wavelength fluorescence spectroscopy. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 127-132 glucose-6-phosphate isomerase Homo sapiens 0-3 8677271-4 1996 pHi in piglet cerebral microvascular endothelial cells in primary culture was monitored using the pH-sensitive fluorescent dye BCECF (2",7"-bis-2-carboxyethyl-5(6)-carboxy-fluorescein acetoxymethyl ester), with dual wavelength fluorescence spectroscopy. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 134-203 glucose-6-phosphate isomerase Homo sapiens 0-3 8677271-5 1996 Endothelial cells attached to coverslips and continuously superfused with HCO3-/CO2 containing medium (25 mM HCO3-, 5% CO2; pH 7.40) have a steady state of pHi of 7.18 +/- 0.02. Bicarbonates 74-78 glucose-6-phosphate isomerase Homo sapiens 156-159 8677271-5 1996 Endothelial cells attached to coverslips and continuously superfused with HCO3-/CO2 containing medium (25 mM HCO3-, 5% CO2; pH 7.40) have a steady state of pHi of 7.18 +/- 0.02. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 80-83 glucose-6-phosphate isomerase Homo sapiens 156-159 8677271-5 1996 Endothelial cells attached to coverslips and continuously superfused with HCO3-/CO2 containing medium (25 mM HCO3-, 5% CO2; pH 7.40) have a steady state of pHi of 7.18 +/- 0.02. Bicarbonates 109-113 glucose-6-phosphate isomerase Homo sapiens 156-159 8677271-6 1996 Under basal conditions, amiloride (100 microMol) and H2DIDS (0.5 mM) decreased pHi 0.12 +/- 0.01 and 0.05 +/- 0.01 pH units, respectively. Amiloride 24-33 glucose-6-phosphate isomerase Homo sapiens 79-82 8677271-6 1996 Under basal conditions, amiloride (100 microMol) and H2DIDS (0.5 mM) decreased pHi 0.12 +/- 0.01 and 0.05 +/- 0.01 pH units, respectively. dihydro-DIDS 53-59 glucose-6-phosphate isomerase Homo sapiens 79-82 8677271-12 1996 pHi recovery during elevated PCO2 was blocked by amiloride, H2DIDS, or Na+-free media. pco2 29-33 glucose-6-phosphate isomerase Homo sapiens 0-3 8677271-12 1996 pHi recovery during elevated PCO2 was blocked by amiloride, H2DIDS, or Na+-free media. Amiloride 49-58 glucose-6-phosphate isomerase Homo sapiens 0-3 8677271-12 1996 pHi recovery during elevated PCO2 was blocked by amiloride, H2DIDS, or Na+-free media. dihydro-DIDS 60-66 glucose-6-phosphate isomerase Homo sapiens 0-3 8679650-3 1996 pHi changes in the colonic adenocarcinoma cell-line SW-620 were recorded by spectrofluorimetric monitoring of the pH-sensitive, fluorescent dye BCECF, and proliferative activity was assessed by [3H]thymidine uptake. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 144-149 glucose-6-phosphate isomerase Homo sapiens 0-3 8679650-3 1996 pHi changes in the colonic adenocarcinoma cell-line SW-620 were recorded by spectrofluorimetric monitoring of the pH-sensitive, fluorescent dye BCECF, and proliferative activity was assessed by [3H]thymidine uptake. Tritium 195-197 glucose-6-phosphate isomerase Homo sapiens 0-3 8679650-3 1996 pHi changes in the colonic adenocarcinoma cell-line SW-620 were recorded by spectrofluorimetric monitoring of the pH-sensitive, fluorescent dye BCECF, and proliferative activity was assessed by [3H]thymidine uptake. Thymidine 198-207 glucose-6-phosphate isomerase Homo sapiens 0-3 8679650-4 1996 Resting pHi in Hepes-buffered solution was 7.53 +/- 0.01 (n = 36). HEPES 15-20 glucose-6-phosphate isomerase Homo sapiens 8-11 8679650-5 1996 Both 1 mM amiloride and Na(+)-free solution inhibited pHi recovery from acidification and decreased pHi in resting cells. Amiloride 10-19 glucose-6-phosphate isomerase Homo sapiens 54-57 8679650-5 1996 Both 1 mM amiloride and Na(+)-free solution inhibited pHi recovery from acidification and decreased pHi in resting cells. Amiloride 10-19 glucose-6-phosphate isomerase Homo sapiens 100-103 8679650-8 1996 In the presence of amiloride and 200 microM H2DIDS pHi recovery was completely inhibited. Amiloride 19-28 glucose-6-phosphate isomerase Homo sapiens 51-54 8679650-10 1996 Addition of 5 microM tributyltin bromide (an anion/OH-exchange ionophore) caused pHi to decrease from 7.43 +/- 0.05 to 7.17 +/- 0.08 (n = 5). Tributyltin bromide 21-40 glucose-6-phosphate isomerase Homo sapiens 81-84 8679650-14 1996 Acid extrusion in physiological bicarbonate media is accomplished by a pHi-sensitive Na+/H+ exchanger and a pHi-insensitive Na(+)-HCO3-cotransporter, both of which are operational in control cells at the resting pHi. Bicarbonates 32-43 glucose-6-phosphate isomerase Homo sapiens 71-74 8725658-1 1996 We present a new convenient method for simultaneous measurement of intracellular calcium concentration ([Ca2+]i) and intracellular pH (pHi) using laser cytometry with a mixture of fluo-3 (for [Ca2+]i) and SNARF-1 (for pHi), with iso excitation (488 nm)-dual emission (530 nm for fluo-3 and > 630 nm for SNARF-1). Fluo-3 180-186 glucose-6-phosphate isomerase Homo sapiens 135-138 8725658-1 1996 We present a new convenient method for simultaneous measurement of intracellular calcium concentration ([Ca2+]i) and intracellular pH (pHi) using laser cytometry with a mixture of fluo-3 (for [Ca2+]i) and SNARF-1 (for pHi), with iso excitation (488 nm)-dual emission (530 nm for fluo-3 and > 630 nm for SNARF-1). Fluo-3 279-285 glucose-6-phosphate isomerase Homo sapiens 135-138 8725658-2 1996 By using this technique, we measured the changes in [Ca2+]i and pHi in A-431 human epidermoid carcinoma cells and HSG human salivary gland cells stimulated by ATP. Adenosine Triphosphate 159-162 glucose-6-phosphate isomerase Homo sapiens 64-67 8725658-4 1996 Using BAPTA (a chelating agent of Ca2+) and amiloride (an inhibitor of the Na+/H+ exchanger), we found that the elevation of pHi requires the elevation of [Ca2+]i but that the elevation of [Ca2+]i does not always require a rise in pHi. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 6-11 glucose-6-phosphate isomerase Homo sapiens 125-128 8725658-4 1996 Using BAPTA (a chelating agent of Ca2+) and amiloride (an inhibitor of the Na+/H+ exchanger), we found that the elevation of pHi requires the elevation of [Ca2+]i but that the elevation of [Ca2+]i does not always require a rise in pHi. Amiloride 44-53 glucose-6-phosphate isomerase Homo sapiens 125-128 8725658-4 1996 Using BAPTA (a chelating agent of Ca2+) and amiloride (an inhibitor of the Na+/H+ exchanger), we found that the elevation of pHi requires the elevation of [Ca2+]i but that the elevation of [Ca2+]i does not always require a rise in pHi. Amiloride 44-53 glucose-6-phosphate isomerase Homo sapiens 231-234 8725658-5 1996 From our results we conclude that elevation of [Ca2+]i takes precedence over the elevation of pHi in ATP-stimulated signal transduction. Adenosine Triphosphate 101-104 glucose-6-phosphate isomerase Homo sapiens 94-97 8766016-0 1996 HCO3-dependent pHi regulation in tracheal epithelial cells. Bicarbonates 0-4 glucose-6-phosphate isomerase Homo sapiens 15-18 8766016-5 1996 This residual Na-independent and HCO3-dependent pHi recovery was studied by using inhibitors of HCO3 and H transporters. Bicarbonates 33-37 glucose-6-phosphate isomerase Homo sapiens 48-51 8766016-5 1996 This residual Na-independent and HCO3-dependent pHi recovery was studied by using inhibitors of HCO3 and H transporters. Bicarbonates 96-100 glucose-6-phosphate isomerase Homo sapiens 48-51 8766016-9 1996 Thus, the HCO3-dependent, Na- and Cl-independent, DPC-blockable pHi recovery may be largely due to an influx of HCO3 via CFTR Cl channels. Bicarbonates 10-14 glucose-6-phosphate isomerase Homo sapiens 64-67 8766016-9 1996 Thus, the HCO3-dependent, Na- and Cl-independent, DPC-blockable pHi recovery may be largely due to an influx of HCO3 via CFTR Cl channels. fenamic acid 50-53 glucose-6-phosphate isomerase Homo sapiens 64-67 8766016-9 1996 Thus, the HCO3-dependent, Na- and Cl-independent, DPC-blockable pHi recovery may be largely due to an influx of HCO3 via CFTR Cl channels. Bicarbonates 112-116 glucose-6-phosphate isomerase Homo sapiens 64-67 8638631-1 1996 We conducted this study to investigate whether antioxidized low-density lipoprotein (a-oxLDL) is an antibody to cryptic and/or neo-antigen on beta2-glycoprotein I (GPI), which is introduced by binding to anionic phospholipid, similar to that of GPI-dependent anticardiolipin antibody (aCL) employing a-oxLDL ELISA. Phospholipids 212-224 glucose-6-phosphate isomerase Homo sapiens 142-162 8638631-1 1996 We conducted this study to investigate whether antioxidized low-density lipoprotein (a-oxLDL) is an antibody to cryptic and/or neo-antigen on beta2-glycoprotein I (GPI), which is introduced by binding to anionic phospholipid, similar to that of GPI-dependent anticardiolipin antibody (aCL) employing a-oxLDL ELISA. Phospholipids 212-224 glucose-6-phosphate isomerase Homo sapiens 164-167 8639816-3 1996 The amino acid sequence of a tryptic peptide and the enzyme cleavage sites revealed 100% homology to neuroleukin or phosphoglucose isomerase (PGI). Peptides 37-44 glucose-6-phosphate isomerase Homo sapiens 101-112 8639816-3 1996 The amino acid sequence of a tryptic peptide and the enzyme cleavage sites revealed 100% homology to neuroleukin or phosphoglucose isomerase (PGI). Peptides 37-44 glucose-6-phosphate isomerase Homo sapiens 116-140 8639816-3 1996 The amino acid sequence of a tryptic peptide and the enzyme cleavage sites revealed 100% homology to neuroleukin or phosphoglucose isomerase (PGI). Peptides 37-44 glucose-6-phosphate isomerase Homo sapiens 142-145 8639816-4 1996 Neuroleukin mediates differentiation of neurons and is homologous to PGI, which catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate. Glucose-6-Phosphate 113-132 glucose-6-phosphate isomerase Homo sapiens 0-11 8639816-4 1996 Neuroleukin mediates differentiation of neurons and is homologous to PGI, which catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate. Glucose-6-Phosphate 113-132 glucose-6-phosphate isomerase Homo sapiens 69-72 8639816-4 1996 Neuroleukin mediates differentiation of neurons and is homologous to PGI, which catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate. fructose-6-phosphate 137-157 glucose-6-phosphate isomerase Homo sapiens 0-11 8639816-4 1996 Neuroleukin mediates differentiation of neurons and is homologous to PGI, which catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate. fructose-6-phosphate 137-157 glucose-6-phosphate isomerase Homo sapiens 69-72 8727026-1 1996 Gastric intramucosal pH (pHi) is often calculated by the Henderson-Hasselbalch equation, using arterial plasma [HCO3-]ap and PCO2 measured in saline obtained from a silastic balloon tonometer after equilibration in the lumen of the stomach. Bicarbonates 112-116 glucose-6-phosphate isomerase Homo sapiens 25-28 8727026-1 1996 Gastric intramucosal pH (pHi) is often calculated by the Henderson-Hasselbalch equation, using arterial plasma [HCO3-]ap and PCO2 measured in saline obtained from a silastic balloon tonometer after equilibration in the lumen of the stomach. Sodium Chloride 142-148 glucose-6-phosphate isomerase Homo sapiens 25-28 8682873-11 1996 Neutrophils spread normally when the oxidase was blocked or when pHi was clamped near physiological values with nigericin. Nigericin 112-121 glucose-6-phosphate isomerase Homo sapiens 65-68 8789815-8 1996 Changes in pHi were induced by 20 or 40 mM propionate. Propionates 43-53 glucose-6-phosphate isomerase Homo sapiens 11-14 8661188-8 1996 For the same level of extracellular acidification, increases in PCO2 were more effective than acute decreases in HCO3- in acidifying pHi and eliciting disturbances in voltage-generating and ion permeability properties of the cell membranes. pco2 64-68 glucose-6-phosphate isomerase Homo sapiens 133-136 8661188-8 1996 For the same level of extracellular acidification, increases in PCO2 were more effective than acute decreases in HCO3- in acidifying pHi and eliciting disturbances in voltage-generating and ion permeability properties of the cell membranes. Bicarbonates 113-117 glucose-6-phosphate isomerase Homo sapiens 133-136 8693442-3 1996 Gastric pHi was calculated from the equation: pHi= 6.1 + log HCO3/(gastric PCO2 X 0.03). Bicarbonates 61-65 glucose-6-phosphate isomerase Homo sapiens 8-11 8693442-3 1996 Gastric pHi was calculated from the equation: pHi= 6.1 + log HCO3/(gastric PCO2 X 0.03). pco2 75-79 glucose-6-phosphate isomerase Homo sapiens 8-11 8967426-0 1996 Palytoxin modulates cytosolic pH in human osteoblast-like Saos-2 cells via an interaction with Na(+)-K(+)-ATPase. palytoxin 0-9 glucose-6-phosphate isomerase Homo sapiens 30-32 8967426-2 1996 In basal human osteoblast-like Saos-2 cells, PTx (8 nM) caused a persistent decrease in cytosolic pH (pHi) of about 0.2 units, which required the presence of extracellular Ca2+ (Cae2+) and Na+ (Nae+). palytoxin 45-48 glucose-6-phosphate isomerase Homo sapiens 98-100 8967426-2 1996 In basal human osteoblast-like Saos-2 cells, PTx (8 nM) caused a persistent decrease in cytosolic pH (pHi) of about 0.2 units, which required the presence of extracellular Ca2+ (Cae2+) and Na+ (Nae+). palytoxin 45-48 glucose-6-phosphate isomerase Homo sapiens 102-105 8967426-4 1996 Under these conditions, PTx increased the pHi without requiring Cae2+ or Nae+, and the alkalinization was unaffected by hexamethylene amiloride. palytoxin 24-27 glucose-6-phosphate isomerase Homo sapiens 42-45 8967426-5 1996 We conclude that the PTx-induced rise in pHi did not involve the Na+/H+ antiporter. palytoxin 21-24 glucose-6-phosphate isomerase Homo sapiens 41-44 8967426-8 1996 Exposure to ouabain had no effect on pHi, but it prevented the actions of PTx on PHi in both basal and nigericin-acidified cells. Ouabain 12-19 glucose-6-phosphate isomerase Homo sapiens 81-84 8967426-8 1996 Exposure to ouabain had no effect on pHi, but it prevented the actions of PTx on PHi in both basal and nigericin-acidified cells. palytoxin 74-77 glucose-6-phosphate isomerase Homo sapiens 81-84 8967426-8 1996 Exposure to ouabain had no effect on pHi, but it prevented the actions of PTx on PHi in both basal and nigericin-acidified cells. Nigericin 103-112 glucose-6-phosphate isomerase Homo sapiens 81-84 8967426-10 1996 We conclude that PTx interacts with the Na(+)-K(+)-adenosinetriphosphatase to regulate pHi in both basal and nigericin-acidified Saos-2 cells. palytoxin 17-20 glucose-6-phosphate isomerase Homo sapiens 87-90 8967426-10 1996 We conclude that PTx interacts with the Na(+)-K(+)-adenosinetriphosphatase to regulate pHi in both basal and nigericin-acidified Saos-2 cells. Nigericin 109-118 glucose-6-phosphate isomerase Homo sapiens 87-90 8789815-7 1996 pHi was measured micro-fluorometrically with the pH-sensitive dye 2",7"-bicarboxyethyl-5(6)-carboxyfluorescein (BCECF), cell volume which is a measure of the net balance between ion influx and efflux was monitored as cell height (CH) and the equivalent short circuit current (Isc) which is a measure of transepithelial K+ secretion was calculated from measurements of the transepithelial voltage (Vt) and the transepithelial resistance (Rt). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 66-110 glucose-6-phosphate isomerase Homo sapiens 0-3 8789815-7 1996 pHi was measured micro-fluorometrically with the pH-sensitive dye 2",7"-bicarboxyethyl-5(6)-carboxyfluorescein (BCECF), cell volume which is a measure of the net balance between ion influx and efflux was monitored as cell height (CH) and the equivalent short circuit current (Isc) which is a measure of transepithelial K+ secretion was calculated from measurements of the transepithelial voltage (Vt) and the transepithelial resistance (Rt). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 112-117 glucose-6-phosphate isomerase Homo sapiens 0-3 8645337-2 1996 Possible interference with mechanisms involving intracellular pH (pHi) regulation was examined by measuring the effect of ET-18-OCH3 on the activity of the Na+/H+ exchanger in the breast cancer-derived cell line MCF-7. edelfosine 122-132 glucose-6-phosphate isomerase Homo sapiens 66-69 8789815-9 1996 In the presence of propionate a transient acidification of pHi was observed in vestibular dark cells as well as a subsequent alkalinization to pHi values exceeding those under control conditions. Propionates 19-29 glucose-6-phosphate isomerase Homo sapiens 59-62 8789815-9 1996 In the presence of propionate a transient acidification of pHi was observed in vestibular dark cells as well as a subsequent alkalinization to pHi values exceeding those under control conditions. Propionates 19-29 glucose-6-phosphate isomerase Homo sapiens 143-146 8789815-10 1996 The alkalinization of pHi in the presence of propionate was inhibited by the NHE blockers amiloride and EIPA. Propionates 45-55 glucose-6-phosphate isomerase Homo sapiens 22-25 8789815-10 1996 The alkalinization of pHi in the presence of propionate was inhibited by the NHE blockers amiloride and EIPA. Amiloride 90-99 glucose-6-phosphate isomerase Homo sapiens 22-25 8789815-10 1996 The alkalinization of pHi in the presence of propionate was inhibited by the NHE blockers amiloride and EIPA. ethylisopropylamiloride 104-108 glucose-6-phosphate isomerase Homo sapiens 22-25 8789815-12 1996 The NHE blocker amiloride caused in vestibular dark cells an acidification of pHi and a decrease in CH. Amiloride 16-25 glucose-6-phosphate isomerase Homo sapiens 78-81 8789815-14 1996 Similar effects and pHi were observed in vestibular dark cells with the amiloride analog ethyl-isopropyl-amiloride (EIPA) and similar effects on Isc were observed with EIPA and the NHE blocker HOE694 when applied to the basolateral side of vestibular dark cell epithelium. Amiloride 72-81 glucose-6-phosphate isomerase Homo sapiens 20-23 8789815-14 1996 Similar effects and pHi were observed in vestibular dark cells with the amiloride analog ethyl-isopropyl-amiloride (EIPA) and similar effects on Isc were observed with EIPA and the NHE blocker HOE694 when applied to the basolateral side of vestibular dark cell epithelium. ethylisopropylamiloride 89-114 glucose-6-phosphate isomerase Homo sapiens 20-23 8621800-16 1996 Differences in pHi, possibly due to lactate differences, between post-rHeEPO and controls appear to be a key factor in the abnormal muscle cell bioenergetics during exercise observed in CRF patients. Lactic Acid 36-43 glucose-6-phosphate isomerase Homo sapiens 15-18 8652331-1 1996 We have calculated gastric intramucosal pH (pHi) from Trip catheter saline tonometered samples in two patients undergoing ventilation using four different sampling techniques, each repeated five times. Sodium Chloride 68-74 glucose-6-phosphate isomerase Homo sapiens 44-47 8662277-4 1996 Trimethylamine (20 mmol/l) increased pHi by 0.78 +/- 0.03 pH units (n = 9) and pH remained stable for the application time. trimethylamine 0-14 glucose-6-phosphate isomerase Homo sapiens 37-40 8662277-13 1996 We conclude that secondary and tertiary amines induce stable alkaline pHi changes, release Ca2+ from intracellular, inositol-1,4, 5-trisphosphate-sensitive Ca2+ stores and increase Ca2+ influx into HT29 cells. Amines 40-46 glucose-6-phosphate isomerase Homo sapiens 70-73 8652331-2 1996 pHi was calculated from measurement of PCO2 in tonometered saline (TCO2). pco2 39-43 glucose-6-phosphate isomerase Homo sapiens 0-3 8652331-2 1996 pHi was calculated from measurement of PCO2 in tonometered saline (TCO2). Sodium Chloride 59-65 glucose-6-phosphate isomerase Homo sapiens 0-3 8652331-2 1996 pHi was calculated from measurement of PCO2 in tonometered saline (TCO2). dioxotechnetium 67-71 glucose-6-phosphate isomerase Homo sapiens 0-3 8814704-5 1996 Based on the magnitude of DNA fragmentation and 3H release at different pHi, it was shown that apoptosis occurred in HL-60 cells when the pHi was lowered from normal pHi of 7.4 to about 7.2-6.7 with a peak increase at pHi 6.8-6.9. Tritium 48-50 glucose-6-phosphate isomerase Homo sapiens 138-141 8926231-4 1996 Methazolamide eliminated the initial overshoot, which also suggested involvement of the initial rapid pHi in the overshoot. Methazolamide 0-13 glucose-6-phosphate isomerase Homo sapiens 102-105 8708169-1 1996 OBJECTIVE: To study the effect of nasogastric suction and ranitidine on the determination of gastric intramucosal pH (pHi). Ranitidine 58-68 glucose-6-phosphate isomerase Homo sapiens 118-121 8708169-9 1996 Compared to control and nasogastric suction periods, after ranitidine mean gastric pHi was higher (control 7.22 +/- 0.08; nasogastric suction 7.23 +/- 0.07; after ranitidine 7.31 +/- 0.06, p < 0.001) mean gastric PCO2 lower (control 6.4 +/- 1.3; nasogastric suction 6.5 +/- 1.3; after ranitidine 5.3 +/- 0.9, p < 0.001) and pH gap lower (control 0.18 +/- 0.08; nasogastric suction 0.17 +/- 0.05; after ranitidine 0.09 +/- 0.06, p < 0.01). Ranitidine 59-69 glucose-6-phosphate isomerase Homo sapiens 83-86 9238683-1 1996 The internal pH (pHi) of human spermatozoa was measured by the fluorescent indicator 2,7-bicarboxyethyl-5,6-carboxyfluorescein acetoxymethyl ester (BCECF-AM) and the distribution of the radioactive [14C]-methylamine under different external ionic conditions. 2,7-bicarboxyethyl-5,6-carboxyfluorescein acetoxymethyl ester 85-146 glucose-6-phosphate isomerase Homo sapiens 17-20 9238683-1 1996 The internal pH (pHi) of human spermatozoa was measured by the fluorescent indicator 2,7-bicarboxyethyl-5,6-carboxyfluorescein acetoxymethyl ester (BCECF-AM) and the distribution of the radioactive [14C]-methylamine under different external ionic conditions. bcecf-am 148-156 glucose-6-phosphate isomerase Homo sapiens 17-20 9238683-1 1996 The internal pH (pHi) of human spermatozoa was measured by the fluorescent indicator 2,7-bicarboxyethyl-5,6-carboxyfluorescein acetoxymethyl ester (BCECF-AM) and the distribution of the radioactive [14C]-methylamine under different external ionic conditions. 14c]-methylamine 199-215 glucose-6-phosphate isomerase Homo sapiens 17-20 8814704-5 1996 Based on the magnitude of DNA fragmentation and 3H release at different pHi, it was shown that apoptosis occurred in HL-60 cells when the pHi was lowered from normal pHi of 7.4 to about 7.2-6.7 with a peak increase at pHi 6.8-6.9. Tritium 48-50 glucose-6-phosphate isomerase Homo sapiens 138-141 8814704-5 1996 Based on the magnitude of DNA fragmentation and 3H release at different pHi, it was shown that apoptosis occurred in HL-60 cells when the pHi was lowered from normal pHi of 7.4 to about 7.2-6.7 with a peak increase at pHi 6.8-6.9. Tritium 48-50 glucose-6-phosphate isomerase Homo sapiens 138-141 8814704-7 1996 Such an increase in apoptosis by ionomycin in HL-60 cells appeared to result from both an increase in [Ca2+]i and from a decline in pHi. Ionomycin 33-42 glucose-6-phosphate isomerase Homo sapiens 132-135 9467248-5 1996 The activity of phosphohexose isomerase remained normal both before and after the glucose load. Glucose 82-89 glucose-6-phosphate isomerase Homo sapiens 16-39 8641338-7 1996 Treatment of monocytes with the intracellular Ca2+ chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA), abolished not only the increase in [Ca2+]i but also the changes in pHi (both acidification and alkalinization) induced by MCAF or FMLP. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 61-117 glucose-6-phosphate isomerase Homo sapiens 194-197 8641338-7 1996 Treatment of monocytes with the intracellular Ca2+ chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA), abolished not only the increase in [Ca2+]i but also the changes in pHi (both acidification and alkalinization) induced by MCAF or FMLP. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 119-124 glucose-6-phosphate isomerase Homo sapiens 194-197 8606923-2 1996 We also added 5-(N-ethyl-N-isopropyl) amiloride (EIPA), a potent inhibitor of one of the major membrane regulators of pHi, the Na+/H+ antiport, and/or removed NaHCO3 to inactivate the alternate membrane regulator of pHi, the HCO3-/Cl- exchanger. ethylisopropylamiloride 14-47 glucose-6-phosphate isomerase Homo sapiens 118-121 8606923-2 1996 We also added 5-(N-ethyl-N-isopropyl) amiloride (EIPA), a potent inhibitor of one of the major membrane regulators of pHi, the Na+/H+ antiport, and/or removed NaHCO3 to inactivate the alternate membrane regulator of pHi, the HCO3-/Cl- exchanger. ethylisopropylamiloride 14-47 glucose-6-phosphate isomerase Homo sapiens 216-219 8606923-2 1996 We also added 5-(N-ethyl-N-isopropyl) amiloride (EIPA), a potent inhibitor of one of the major membrane regulators of pHi, the Na+/H+ antiport, and/or removed NaHCO3 to inactivate the alternate membrane regulator of pHi, the HCO3-/Cl- exchanger. ethylisopropylamiloride 49-53 glucose-6-phosphate isomerase Homo sapiens 118-121 8606923-2 1996 We also added 5-(N-ethyl-N-isopropyl) amiloride (EIPA), a potent inhibitor of one of the major membrane regulators of pHi, the Na+/H+ antiport, and/or removed NaHCO3 to inactivate the alternate membrane regulator of pHi, the HCO3-/Cl- exchanger. ethylisopropylamiloride 49-53 glucose-6-phosphate isomerase Homo sapiens 216-219 9388330-1 1996 We studied the correlation of intramucosal pH (pHi), oxygen delivery (DO2) and oxygen consumption (VO2) in 21 patients with septic shock, and found that pHi decreased dramatically (P < 0.05) in nonsurvivors while it had no significant change in survivors. Oxygen 53-59 glucose-6-phosphate isomerase Homo sapiens 153-156 9388330-1 1996 We studied the correlation of intramucosal pH (pHi), oxygen delivery (DO2) and oxygen consumption (VO2) in 21 patients with septic shock, and found that pHi decreased dramatically (P < 0.05) in nonsurvivors while it had no significant change in survivors. do2 70-73 glucose-6-phosphate isomerase Homo sapiens 153-156 9388330-1 1996 We studied the correlation of intramucosal pH (pHi), oxygen delivery (DO2) and oxygen consumption (VO2) in 21 patients with septic shock, and found that pHi decreased dramatically (P < 0.05) in nonsurvivors while it had no significant change in survivors. Oxygen 79-85 glucose-6-phosphate isomerase Homo sapiens 153-156 9388330-1 1996 We studied the correlation of intramucosal pH (pHi), oxygen delivery (DO2) and oxygen consumption (VO2) in 21 patients with septic shock, and found that pHi decreased dramatically (P < 0.05) in nonsurvivors while it had no significant change in survivors. vo2 99-102 glucose-6-phosphate isomerase Homo sapiens 153-156 9388330-2 1996 In survivors, there was a positive correlation between pHi and DO2 (r = 0.71, P < 0.001), pHi and VO2 (r = 0.63, P < 0.001) when DO2 was below the critical level of 641 ml/min/m2. do2 63-66 glucose-6-phosphate isomerase Homo sapiens 55-58 9388330-4 1996 The correlation between pHi and DO2 proves the oxygen supply dependency of VO2. do2 32-35 glucose-6-phosphate isomerase Homo sapiens 24-27 9388330-4 1996 The correlation between pHi and DO2 proves the oxygen supply dependency of VO2. Oxygen 47-53 glucose-6-phosphate isomerase Homo sapiens 24-27 9388330-6 1996 pHi is a reliable and useful parameter not only for prognosis, but also for titrating the therapy in septic shock if pHi is monitored serially together with DO2. do2 157-160 glucose-6-phosphate isomerase Homo sapiens 0-3 8611598-2 1996 Basal pH(i) was higher in HC(O3-)- buffered solutions (7(7.33 +/- 0.01) than in nominally HCO3- free, Hepes buffered solutions (7.16 +/- 0.01, P < 0.05). Bicarbonates 26-31 glucose-6-phosphate isomerase Homo sapiens 6-11 8611598-7 1996 The stilbene derivative SITS further inhibited the EIPA-resistant pH(i) recovery. Stilbenes 4-12 glucose-6-phosphate isomerase Homo sapiens 66-71 7485098-1 1995 The hematologic disorder paroxysmal nocturnal hemoglobinuria (PNH) arises from a somatic mutation within the Piga gene important for the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. Glycosylphosphatidylinositols 153-181 glucose-6-phosphate isomerase Homo sapiens 183-186 8805791-6 1996 Specifically, inhibition of the Na+/H+ exchanger with dimethylamiloride or HOE694 delayed the return of physiologic pH(i) after reperfusion and prevented reperfusion-induced cell killing to both cultured myocytes and perfused papillary muscle. 5-dimethylamiloride 54-71 glucose-6-phosphate isomerase Homo sapiens 116-118 8805791-6 1996 Specifically, inhibition of the Na+/H+ exchanger with dimethylamiloride or HOE694 delayed the return of physiologic pH(i) after reperfusion and prevented reperfusion-induced cell killing to both cultured myocytes and perfused papillary muscle. 3-methylsulfonyl-4-piperidinobenzoyl guanidine 75-81 glucose-6-phosphate isomerase Homo sapiens 116-118 8805791-12 1996 Increasing pH(i) may also induce a pH-dependent mitochondrial permeability transition and activate the myofibrillar ATPase, effects that increase ATP demand and compromise ATP supply. Adenosine Triphosphate 116-119 glucose-6-phosphate isomerase Homo sapiens 11-13 8805791-12 1996 Increasing pH(i) may also induce a pH-dependent mitochondrial permeability transition and activate the myofibrillar ATPase, effects that increase ATP demand and compromise ATP supply. Adenosine Triphosphate 146-149 glucose-6-phosphate isomerase Homo sapiens 11-13 8805791-12 1996 Increasing pH(i) may also induce a pH-dependent mitochondrial permeability transition and activate the myofibrillar ATPase, effects that increase ATP demand and compromise ATP supply. Adenosine Triphosphate 146-149 glucose-6-phosphate isomerase Homo sapiens 35-37 9238652-4 1996 In this study, flow cytometric analysis of pHi in human spermatozoa was accomplished by using one of the new benzo[c]xanthene dyes (SNAFL-1). benzo[c]xanthene 109-125 glucose-6-phosphate isomerase Homo sapiens 43-46 8883028-3 1996 The initial rate of pHi recovery immediately after intracellular acidification with 100 mmol/l propionic acid, representing the maximum Na+/H+ exchange activity, was significantly higher in lymphocytes from patients with mild chronic renal failure (7.10 +/- 0.52 dpHi/s, mean +/- SEM) when compared with control subjects (5.42 +/- 0.47 dpHi/s; p < 0.05). propionic acid 95-109 glucose-6-phosphate isomerase Homo sapiens 20-23 7589503-5 1995 We have found that N-ethylmaleimide and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, known as inhibitors of V-type H+ ATPase, inhibit the elevation of pHi induced by cell-cell contact interactions; meanwhile Cd2+ ions, which inhibit H+ conductive pathway, cause an increase of pHi in a confluent monolayer. 7-chloro-4-nitrobenz-2-oxa-1,3-diazole 40-78 glucose-6-phosphate isomerase Homo sapiens 146-149 8542195-1 1995 It has been proposed that in mammalian systems the glucose analog 2-fluoro-2-deoxy-D-glucose (FDG) is phosphorylated and subsequently converted to the corresponding mannose derivative via the action of phosphoglucose isomerase. Glucose 51-58 glucose-6-phosphate isomerase Homo sapiens 202-226 8542195-1 1995 It has been proposed that in mammalian systems the glucose analog 2-fluoro-2-deoxy-D-glucose (FDG) is phosphorylated and subsequently converted to the corresponding mannose derivative via the action of phosphoglucose isomerase. Fluorodeoxyglucose F18 66-92 glucose-6-phosphate isomerase Homo sapiens 202-226 8542195-1 1995 It has been proposed that in mammalian systems the glucose analog 2-fluoro-2-deoxy-D-glucose (FDG) is phosphorylated and subsequently converted to the corresponding mannose derivative via the action of phosphoglucose isomerase. Mannose 165-172 glucose-6-phosphate isomerase Homo sapiens 202-226 8542195-4 1995 Formation of the corresponding C-6 phosphorylated 2-FDG analog with hexokinase, followed by treatment of the resulting phosphorylated products with phosphoglucose isomerase, resulted in the observation of additional 19F resonances consistent with the corresponding 2-fluoro-2-deoxy-D-mannose-6-phosphate metabolite. Carbon 31-32 glucose-6-phosphate isomerase Homo sapiens 148-172 8542195-4 1995 Formation of the corresponding C-6 phosphorylated 2-FDG analog with hexokinase, followed by treatment of the resulting phosphorylated products with phosphoglucose isomerase, resulted in the observation of additional 19F resonances consistent with the corresponding 2-fluoro-2-deoxy-D-mannose-6-phosphate metabolite. 2-fdg 50-55 glucose-6-phosphate isomerase Homo sapiens 148-172 8599248-8 1995 The intracellular pH (pHi) of HL-60 cells was 6.6-6.9 when the extracellular pH (pHe) was 6.2-6.4. Phenylalanine 81-84 glucose-6-phosphate isomerase Homo sapiens 22-25 7554137-3 1995 The oral glucose challenge significantly increased plasma glucose, plasma insulin, and the lymphocytic Na(+)-H+ exchange activity, measured as change of pHi per second (control [0 hours], 5.20 +/- 0.53 x 10(-3) dpHi/s; 1 hour after glucose administration, 8.28 +/- 1.07 x 10(-3) dpHi/s; 2 hours after glucose administration, 8.15 +/- 1.18 x 10(-3) dpHi/s; P = .002). Sulfur 27-28 glucose-6-phosphate isomerase Homo sapiens 153-156 7554137-3 1995 The oral glucose challenge significantly increased plasma glucose, plasma insulin, and the lymphocytic Na(+)-H+ exchange activity, measured as change of pHi per second (control [0 hours], 5.20 +/- 0.53 x 10(-3) dpHi/s; 1 hour after glucose administration, 8.28 +/- 1.07 x 10(-3) dpHi/s; 2 hours after glucose administration, 8.15 +/- 1.18 x 10(-3) dpHi/s; P = .002). Sulfur 27-28 glucose-6-phosphate isomerase Homo sapiens 153-156 7478437-1 1995 The effect of hyperthermia at 43 degrees C on intracellular pH (pHi) in human U-87 MG glioblastoma cells was studied by using the fluorescent probe 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein-pentaacetoxymethyl ester. 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein-pentaacetoxymethyl ester 148-220 glucose-6-phosphate isomerase Homo sapiens 64-67 7478437-4 1995 The acidification was readily reversible by cooling the cells back down to 37 degrees C. The pHi change was inhibited by the addition of 1 mM amiloride in the incubation medium. Amiloride 142-151 glucose-6-phosphate isomerase Homo sapiens 93-96 8542195-4 1995 Formation of the corresponding C-6 phosphorylated 2-FDG analog with hexokinase, followed by treatment of the resulting phosphorylated products with phosphoglucose isomerase, resulted in the observation of additional 19F resonances consistent with the corresponding 2-fluoro-2-deoxy-D-mannose-6-phosphate metabolite. mannose-6-phosphate 281-303 glucose-6-phosphate isomerase Homo sapiens 148-172 8607311-1 1995 The effect of low-dose dopexamine and dopamine on gastric intramucosal pH (pHi) during cardiac surgery and 16 hours postoperatively was studied in 35 adults patients (coronary artery bypass grafting and/or valve replacement). dopexamine 23-33 glucose-6-phosphate isomerase Homo sapiens 75-78 8607311-1 1995 The effect of low-dose dopexamine and dopamine on gastric intramucosal pH (pHi) during cardiac surgery and 16 hours postoperatively was studied in 35 adults patients (coronary artery bypass grafting and/or valve replacement). Dopamine 38-46 glucose-6-phosphate isomerase Homo sapiens 75-78 8607311-8 1995 The observed changes in pHi and PtonCO2 were due to changes in pHa and in PaCO2 and not a sign of gastric mucosal ischemia. paco2 74-79 glucose-6-phosphate isomerase Homo sapiens 24-27 7554137-3 1995 The oral glucose challenge significantly increased plasma glucose, plasma insulin, and the lymphocytic Na(+)-H+ exchange activity, measured as change of pHi per second (control [0 hours], 5.20 +/- 0.53 x 10(-3) dpHi/s; 1 hour after glucose administration, 8.28 +/- 1.07 x 10(-3) dpHi/s; 2 hours after glucose administration, 8.15 +/- 1.18 x 10(-3) dpHi/s; P = .002). Glucose 9-16 glucose-6-phosphate isomerase Homo sapiens 153-156 7554137-3 1995 The oral glucose challenge significantly increased plasma glucose, plasma insulin, and the lymphocytic Na(+)-H+ exchange activity, measured as change of pHi per second (control [0 hours], 5.20 +/- 0.53 x 10(-3) dpHi/s; 1 hour after glucose administration, 8.28 +/- 1.07 x 10(-3) dpHi/s; 2 hours after glucose administration, 8.15 +/- 1.18 x 10(-3) dpHi/s; P = .002). Sulfur 14-15 glucose-6-phosphate isomerase Homo sapiens 153-156 7589503-5 1995 We have found that N-ethylmaleimide and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, known as inhibitors of V-type H+ ATPase, inhibit the elevation of pHi induced by cell-cell contact interactions; meanwhile Cd2+ ions, which inhibit H+ conductive pathway, cause an increase of pHi in a confluent monolayer. Ethylmaleimide 19-35 glucose-6-phosphate isomerase Homo sapiens 146-149 7589503-5 1995 We have found that N-ethylmaleimide and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, known as inhibitors of V-type H+ ATPase, inhibit the elevation of pHi induced by cell-cell contact interactions; meanwhile Cd2+ ions, which inhibit H+ conductive pathway, cause an increase of pHi in a confluent monolayer. Ethylmaleimide 19-35 glucose-6-phosphate isomerase Homo sapiens 272-275 7589503-5 1995 We have found that N-ethylmaleimide and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole, known as inhibitors of V-type H+ ATPase, inhibit the elevation of pHi induced by cell-cell contact interactions; meanwhile Cd2+ ions, which inhibit H+ conductive pathway, cause an increase of pHi in a confluent monolayer. 7-chloro-4-nitrobenz-2-oxa-1,3-diazole 40-78 glucose-6-phosphate isomerase Homo sapiens 272-275 7589503-7 1995 Inhibitors of phospholipase A2 (4-bromophenacyl-bromide), phospholipase C (neomycin) and protein kinase C (H-7) dramatically change the way the pHi is modulated by local cell density. 4-bromophenacyl bromide 32-55 glucose-6-phosphate isomerase Homo sapiens 144-147 7589503-7 1995 Inhibitors of phospholipase A2 (4-bromophenacyl-bromide), phospholipase C (neomycin) and protein kinase C (H-7) dramatically change the way the pHi is modulated by local cell density. Neomycin 75-83 glucose-6-phosphate isomerase Homo sapiens 144-147 7485540-4 1995 The rate of cellular NH3 influx was calculated from the time course of increase in intracellular pH (pHi), measured with 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein after 20 mM NH4Cl was added to the bath or luminal perfusate. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 121-168 glucose-6-phosphate isomerase Homo sapiens 101-104 7568166-6 1995 Parallel measurements of intracellular pH (pHi) using the fluorescent dye 2",7"-bis(2-carboxyethyl)-5-(and -6)-carboxyfluorescein (BCECF) in every case showed a 0.15- to 0.2-pH-unit alkalinization in ABA, demonstrating that the transgene was without effect on the pHi signal that mediates in ABA-evoked K+ channel control. 2",7"-bis(2-carboxyethyl)-5-(and -6)-carboxyfluorescein 74-129 glucose-6-phosphate isomerase Homo sapiens 43-46 7568166-8 1995 These results demonstrate an aberrant K+ channel behavior--including channel insensitivity to ABA-dependent alkalinization of pHi--as a major consequence of abi1-1 action and implicate AB11 as part of a phosphatase/kinase pathway that modulates the sensitivity of guard-cell K+ channels to ABA-evoked signal cascades. Abscisic Acid 94-97 glucose-6-phosphate isomerase Homo sapiens 126-129 7575397-3 1995 In HCO3(-)-free buffer (pH = 7.4), ET-1 (0.1-50 nM) induced a sustained, dose-dependent increase in pHi. Bicarbonates 3-8 glucose-6-phosphate isomerase Homo sapiens 100-103 8558591-2 1995 The cytosolic pH (pHi) was measured microfluorometrically with the pH-sensitive dye 2",7"-bicarboxyethyl-5(6)-carboxyfluorescein (BCECF) and the equivalent short-circuit current (Isc), which is a measure for transepithelial K+ secretion, was calculated from measurements of the transepithelial voltage (Vt) and the transepithelial resistance (Rt) in a micro-Ussing chamber. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 84-128 glucose-6-phosphate isomerase Homo sapiens 18-21 8558591-2 1995 The cytosolic pH (pHi) was measured microfluorometrically with the pH-sensitive dye 2",7"-bicarboxyethyl-5(6)-carboxyfluorescein (BCECF) and the equivalent short-circuit current (Isc), which is a measure for transepithelial K+ secretion, was calculated from measurements of the transepithelial voltage (Vt) and the transepithelial resistance (Rt) in a micro-Ussing chamber. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 130-135 glucose-6-phosphate isomerase Homo sapiens 18-21 8558591-2 1995 The cytosolic pH (pHi) was measured microfluorometrically with the pH-sensitive dye 2",7"-bicarboxyethyl-5(6)-carboxyfluorescein (BCECF) and the equivalent short-circuit current (Isc), which is a measure for transepithelial K+ secretion, was calculated from measurements of the transepithelial voltage (Vt) and the transepithelial resistance (Rt) in a micro-Ussing chamber. isc 179-182 glucose-6-phosphate isomerase Homo sapiens 18-21 8558591-5 1995 Addition of 20 mM propionate- caused a biphasic effect involving an initial acidification of pHi, increase in Vt and Isc and decrease in Rt and a subsequent alkalinization of pHi, decrease of Vt and increase of Rt. Propionates 18-28 glucose-6-phosphate isomerase Homo sapiens 93-96 8558591-5 1995 Addition of 20 mM propionate- caused a biphasic effect involving an initial acidification of pHi, increase in Vt and Isc and decrease in Rt and a subsequent alkalinization of pHi, decrease of Vt and increase of Rt. Propionates 18-28 glucose-6-phosphate isomerase Homo sapiens 175-178 8558591-6 1995 Removal of propionate- caused a transient effect involving an alkalinization of pHi, a decrease of Vt and Isc and an increase in Rt, pHi in the presence of propionate- exceeded pHi under control conditions. Propionates 11-21 glucose-6-phosphate isomerase Homo sapiens 80-83 8558591-6 1995 Removal of propionate- caused a transient effect involving an alkalinization of pHi, a decrease of Vt and Isc and an increase in Rt, pHi in the presence of propionate- exceeded pHi under control conditions. Propionates 11-21 glucose-6-phosphate isomerase Homo sapiens 133-136 8558591-6 1995 Removal of propionate- caused a transient effect involving an alkalinization of pHi, a decrease of Vt and Isc and an increase in Rt, pHi in the presence of propionate- exceeded pHi under control conditions. Propionates 11-21 glucose-6-phosphate isomerase Homo sapiens 133-136 8558591-6 1995 Removal of propionate- caused a transient effect involving an alkalinization of pHi, a decrease of Vt and Isc and an increase in Rt, pHi in the presence of propionate- exceeded pHi under control conditions. Propionates 156-166 glucose-6-phosphate isomerase Homo sapiens 80-83 8558591-6 1995 Removal of propionate- caused a transient effect involving an alkalinization of pHi, a decrease of Vt and Isc and an increase in Rt, pHi in the presence of propionate- exceeded pHi under control conditions. Propionates 156-166 glucose-6-phosphate isomerase Homo sapiens 133-136 8558591-6 1995 Removal of propionate- caused a transient effect involving an alkalinization of pHi, a decrease of Vt and Isc and an increase in Rt, pHi in the presence of propionate- exceeded pHi under control conditions. Propionates 156-166 glucose-6-phosphate isomerase Homo sapiens 133-136 7575397-6 1995 Recovery of pHi in response to acidification, induced by washout of a 20 mM NH4Cl prepulse, was > 90% inhibited by EIPA (3 microM), confirming the presence of an ET-1-responsive Na(+)-H+ exchanger. Ammonium Chloride 76-81 glucose-6-phosphate isomerase Homo sapiens 12-15 7572007-1 1995 Gastric tonometry was used to study the possible effect of dopexamine infusion on a low calculated intramucosal pH (pHi) as a sign of splanchnic ischemia. dopexamine 59-69 glucose-6-phosphate isomerase Homo sapiens 116-119 7635429-4 1995 Amiloride (1 mmol/L) and external Na+ removal decreased baseline pHi in both HEPES and KRB. Amiloride 0-9 glucose-6-phosphate isomerase Homo sapiens 65-68 7635429-4 1995 Amiloride (1 mmol/L) and external Na+ removal decreased baseline pHi in both HEPES and KRB. HEPES 77-82 glucose-6-phosphate isomerase Homo sapiens 65-68 7635429-4 1995 Amiloride (1 mmol/L) and external Na+ removal decreased baseline pHi in both HEPES and KRB. krb 87-90 glucose-6-phosphate isomerase Homo sapiens 65-68 7635429-8 1995 Administration of EGF and TGF alpha, but not of IGF-II, induced a dose-dependent, amiloride-inhibitable increase in baseline pHi, together with an increase in Na+/H+ exchange activity, shifting to the right the JH/pHi curve. Amiloride 82-91 glucose-6-phosphate isomerase Homo sapiens 125-128 7635429-8 1995 Administration of EGF and TGF alpha, but not of IGF-II, induced a dose-dependent, amiloride-inhibitable increase in baseline pHi, together with an increase in Na+/H+ exchange activity, shifting to the right the JH/pHi curve. Amiloride 82-91 glucose-6-phosphate isomerase Homo sapiens 214-217 8537444-1 1995 Application of extracellular adenosine triphosphate (ATP) induces a pulsed decrease in osteoclast intracellular pH (pHi), as measured with seminaphthofluorescein (SNAFL)-calcein on a laser scanning confocal microscope. Adenosine Triphosphate 29-51 glucose-6-phosphate isomerase Homo sapiens 116-119 8537444-1 1995 Application of extracellular adenosine triphosphate (ATP) induces a pulsed decrease in osteoclast intracellular pH (pHi), as measured with seminaphthofluorescein (SNAFL)-calcein on a laser scanning confocal microscope. Adenosine Triphosphate 53-56 glucose-6-phosphate isomerase Homo sapiens 116-119 8537444-2 1995 Adenosine diphosphate also produces a pHi decrease, but adenosine monophosphate, uridine triphosphate, 2-methylthio-ATP, and beta, gamma-methylene-ATP have little effect on pHi. Adenosine Diphosphate 0-21 glucose-6-phosphate isomerase Homo sapiens 38-41 8537444-3 1995 The ATP-induced pHi decrease is largely inhibited by suramin, a P2 purinergic receptor blocker. Adenosine Triphosphate 4-7 glucose-6-phosphate isomerase Homo sapiens 16-19 8537444-3 1995 The ATP-induced pHi decrease is largely inhibited by suramin, a P2 purinergic receptor blocker. Suramin 53-60 glucose-6-phosphate isomerase Homo sapiens 16-19 8537444-7 1995 Two inhibitors of the osteoclast cell membrane proton pump, N-ethylmaleimide and vanadate, produce partial inhibition of the ATP-induced pHi decrease. Ethylmaleimide 60-76 glucose-6-phosphate isomerase Homo sapiens 137-140 8537444-7 1995 Two inhibitors of the osteoclast cell membrane proton pump, N-ethylmaleimide and vanadate, produce partial inhibition of the ATP-induced pHi decrease. Vanadates 81-89 glucose-6-phosphate isomerase Homo sapiens 137-140 8537444-7 1995 Two inhibitors of the osteoclast cell membrane proton pump, N-ethylmaleimide and vanadate, produce partial inhibition of the ATP-induced pHi decrease. Adenosine Triphosphate 125-128 glucose-6-phosphate isomerase Homo sapiens 137-140 8537444-9 1995 None of the proton pump inhibitors but vanadate has a direct effect on pHi. Vanadates 39-47 glucose-6-phosphate isomerase Homo sapiens 71-74 8537444-10 1995 Vanadate produces a transient pHi increase upon application to the bathing medium, possibly as a result of its known effect of stimulating the Na+/H+ exchanger. Vanadates 0-8 glucose-6-phosphate isomerase Homo sapiens 30-33 8537444-11 1995 Inhibition of Cl-/HCO3- exchange by decreasing extracellular Cl- gives a pronounced long-term pHi increase, supporting the hypothesis that this exchange has an important role in osteoclast pHi homeostasis. Bicarbonates 18-22 glucose-6-phosphate isomerase Homo sapiens 94-97 8537444-11 1995 Inhibition of Cl-/HCO3- exchange by decreasing extracellular Cl- gives a pronounced long-term pHi increase, supporting the hypothesis that this exchange has an important role in osteoclast pHi homeostasis. Bicarbonates 18-22 glucose-6-phosphate isomerase Homo sapiens 189-192 8537444-12 1995 In Cl(-)-free extracellular medium, there is a greatly reduced effect of extracellular ATP on pHi. Adenosine Triphosphate 87-90 glucose-6-phosphate isomerase Homo sapiens 94-97 7579086-1 1995 Commercial peritoneal dialysis solution (CDS) is known to have a detrimental effect on the capacity of peritoneal macrophages (PM phi) to kill bacteria and produce acute phase cytokines. cds 41-44 glucose-6-phosphate isomerase Homo sapiens 130-133 7579086-3 1995 Because the cytoplasmic pH (pHi) is an important determinant of cellular function, the effect of CDS on the pHi of PM phi from continuous ambulatory peritoneal dialysis patients was studied. cds 97-100 glucose-6-phosphate isomerase Homo sapiens 108-111 7579086-3 1995 Because the cytoplasmic pH (pHi) is an important determinant of cellular function, the effect of CDS on the pHi of PM phi from continuous ambulatory peritoneal dialysis patients was studied. cds 97-100 glucose-6-phosphate isomerase Homo sapiens 118-121 7579086-4 1995 The pHi of PM phi was measured fluorometrically in N-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered salt solution (HBSS) or CDS at pH values of 5.3, 6.5, and 7.0, values that represent the pH existing in dialysate during the first 30 min of dwell time. n-hydroxyethylpiperazine-n"-2-ethanesulfonic acid 51-100 glucose-6-phosphate isomerase Homo sapiens 4-7 7579086-4 1995 The pHi of PM phi was measured fluorometrically in N-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered salt solution (HBSS) or CDS at pH values of 5.3, 6.5, and 7.0, values that represent the pH existing in dialysate during the first 30 min of dwell time. n-hydroxyethylpiperazine-n"-2-ethanesulfonic acid 51-100 glucose-6-phosphate isomerase Homo sapiens 14-17 7579086-4 1995 The pHi of PM phi was measured fluorometrically in N-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered salt solution (HBSS) or CDS at pH values of 5.3, 6.5, and 7.0, values that represent the pH existing in dialysate during the first 30 min of dwell time. TVZ 7 102-107 glucose-6-phosphate isomerase Homo sapiens 4-7 7579086-4 1995 The pHi of PM phi was measured fluorometrically in N-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered salt solution (HBSS) or CDS at pH values of 5.3, 6.5, and 7.0, values that represent the pH existing in dialysate during the first 30 min of dwell time. TVZ 7 102-107 glucose-6-phosphate isomerase Homo sapiens 14-17 7579086-4 1995 The pHi of PM phi was measured fluorometrically in N-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered salt solution (HBSS) or CDS at pH values of 5.3, 6.5, and 7.0, values that represent the pH existing in dialysate during the first 30 min of dwell time. Salts 118-122 glucose-6-phosphate isomerase Homo sapiens 4-7 7579086-4 1995 The pHi of PM phi was measured fluorometrically in N-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered salt solution (HBSS) or CDS at pH values of 5.3, 6.5, and 7.0, values that represent the pH existing in dialysate during the first 30 min of dwell time. hbss 133-137 glucose-6-phosphate isomerase Homo sapiens 4-7 7579086-4 1995 The pHi of PM phi was measured fluorometrically in N-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered salt solution (HBSS) or CDS at pH values of 5.3, 6.5, and 7.0, values that represent the pH existing in dialysate during the first 30 min of dwell time. hbss 133-137 glucose-6-phosphate isomerase Homo sapiens 14-17 7579086-5 1995 For any given pH of the experimental medium, the pHi was always more acidic in CDS than in HBSS. cds 79-82 glucose-6-phosphate isomerase Homo sapiens 49-52 7579086-5 1995 For any given pH of the experimental medium, the pHi was always more acidic in CDS than in HBSS. hbss 91-95 glucose-6-phosphate isomerase Homo sapiens 49-52 7579086-6 1995 When PM phi were incubated with a lactate-containing HBSS, a cellular acidification was observed that was similar to that attained by exposure to CDS at the same pH. Lactic Acid 34-41 glucose-6-phosphate isomerase Homo sapiens 8-11 7579086-6 1995 When PM phi were incubated with a lactate-containing HBSS, a cellular acidification was observed that was similar to that attained by exposure to CDS at the same pH. hbss 53-57 glucose-6-phosphate isomerase Homo sapiens 8-11 7579086-7 1995 This supports the hypothesis that the decrease in pHi was due to the influx of lactic acid from the CDS into the PM phi. Lactic Acid 79-90 glucose-6-phosphate isomerase Homo sapiens 50-53 7579086-7 1995 This supports the hypothesis that the decrease in pHi was due to the influx of lactic acid from the CDS into the PM phi. Lactic Acid 79-90 glucose-6-phosphate isomerase Homo sapiens 116-119 7579086-7 1995 This supports the hypothesis that the decrease in pHi was due to the influx of lactic acid from the CDS into the PM phi. cds 100-103 glucose-6-phosphate isomerase Homo sapiens 50-53 7579086-7 1995 This supports the hypothesis that the decrease in pHi was due to the influx of lactic acid from the CDS into the PM phi. cds 100-103 glucose-6-phosphate isomerase Homo sapiens 116-119 7579086-8 1995 In order to demonstrate a causal association between the CDS-induced cellular acidification and a defect in phagocytosis and cytokine production, these functions were studied after pHi clamping by means of K+/nigericin. cds 57-60 glucose-6-phosphate isomerase Homo sapiens 181-184 7572007-6 1995 After 4 hours a significant (P < 0.05) decrease in pHi was noted in the dopexamine group which remained significantly below the placebo group during the monitoring period. dopexamine 75-85 glucose-6-phosphate isomerase Homo sapiens 54-57 7572007-7 1995 The dopexamine treated patients had a significantly longer period of low pHi but the pH-gap i.e. the difference between arterial pH and pHi did not differ between the two groups. dopexamine 4-14 glucose-6-phosphate isomerase Homo sapiens 73-76 7572018-3 1995 This prospective study was designed to determine if stress ulcer prophylaxis with sucralfate interferes with pHi measurement. Sucralfate 82-92 glucose-6-phosphate isomerase Homo sapiens 109-112 7648106-0 1995 Effect of low-dose dopamine on sigmoid colonic intramucosal pH in patients undergoing elective abdominal aortic aneurysm repair. Dopamine 19-27 glucose-6-phosphate isomerase Homo sapiens 60-62 7648106-1 1995 The effect of low-dose dopamine administration on intramucosal pH (pHi) of the sigmoid colon and on postoperative function of various organs in patients undergoing elective abdominal aortic aneurysm repair was examined. Dopamine 23-31 glucose-6-phosphate isomerase Homo sapiens 63-65 7648106-1 1995 The effect of low-dose dopamine administration on intramucosal pH (pHi) of the sigmoid colon and on postoperative function of various organs in patients undergoing elective abdominal aortic aneurysm repair was examined. Dopamine 23-31 glucose-6-phosphate isomerase Homo sapiens 67-70 7648106-5 1995 pHi fell to a significantly lower minimum value in those receiving dopamine compared with control patients (6.86(0.10) versus 7.11(0.08), P < 0.05). Dopamine 67-75 glucose-6-phosphate isomerase Homo sapiens 0-3 7778066-6 1995 The pHi-stimulating effect of endothelin-1 was inhibited by 10(-7) M staurosporine, calphostin C and 5-(N,N-hexamethylene) amiloride. Staurosporine 69-82 glucose-6-phosphate isomerase Homo sapiens 4-7 7606962-12 1995 The pHi, but not PiCO2 correlated with extraction ratio and lactate level. Lactic Acid 60-67 glucose-6-phosphate isomerase Homo sapiens 4-7 7790401-1 1995 A new pH indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular pH (pHi) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter set. seminaphthofluorescein 20-42 glucose-6-phosphate isomerase Homo sapiens 111-114 7790401-1 1995 A new pH indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular pH (pHi) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter set. seminaphthofluorescein 44-49 glucose-6-phosphate isomerase Homo sapiens 111-114 7790401-1 1995 A new pH indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular pH (pHi) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter set. calcein AM 51-78 glucose-6-phosphate isomerase Homo sapiens 111-114 7790401-1 1995 A new pH indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular pH (pHi) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter set. Argon 215-220 glucose-6-phosphate isomerase Homo sapiens 111-114 7790401-1 1995 A new pH indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular pH (pHi) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter set. Fluorescein-5-isothiocyanate 274-278 glucose-6-phosphate isomerase Homo sapiens 111-114 7790401-1 1995 A new pH indicator, seminaphthofluorescein (SNAFL)-calcein acetoxymethyl ester, was used for intracellular pH (pHi) measurement in living MDCK cells with a laser scanning confocal microscope (LSCM) equipped with an Argon/Krypton laser and dual-excitation and dual-emission (FITC/Texas Red) filter set. Texas red 279-288 glucose-6-phosphate isomerase Homo sapiens 111-114 8544928-0 1995 [Effect of intravenous glucose load on blood glucose and fructose level and glucose-6-phosphate isomerase activity in serum of patients with acute cerebrovascular disease in its earliest phase]. Glucose 23-30 glucose-6-phosphate isomerase Homo sapiens 76-105 7614000-0 1995 The influence of calcium transients on intracellular pH in cortical neurons in primary culture. Calcium 17-24 glucose-6-phosphate isomerase Homo sapiens 53-55 7614000-4 1995 Glutamate exposure either caused no, or only a small decrease in pHi (delta pH approximately 0.06 units). Glutamic Acid 0-9 glucose-6-phosphate isomerase Homo sapiens 65-68 7614000-4 1995 Glutamate exposure either caused no, or only a small decrease in pHi (delta pH approximately 0.06 units). Glutamic Acid 0-9 glucose-6-phosphate isomerase Homo sapiens 65-67 7614000-5 1995 When a decrease was observed, a rebound rise in pHi above control was observed upon termination of glutamate exposure. Glutamic Acid 99-108 glucose-6-phosphate isomerase Homo sapiens 48-51 7614000-7 1995 Exposure of cells to 50 mM KCl consistently increased pHi. Potassium Chloride 27-30 glucose-6-phosphate isomerase Homo sapiens 54-57 7698616-2 1995 This study assessed the roles of Na+, H+, and HCO3- transport mechanisms in controlling pHi during short-term exposure of the gastric epithelium to luminal acid. Bicarbonates 46-50 glucose-6-phosphate isomerase Homo sapiens 88-91 7698616-6 1995 Blocking of Na+/H+ exchange (in the presence of HCO3-/CO2) by removal of Na+ or addition of amiloride eliminated the increase in aiNa and resulted in uncontrolled acidification of pHi. Amiloride 92-101 glucose-6-phosphate isomerase Homo sapiens 180-183 7698616-7 1995 Similarly, blocking of HCO3- transport (in the presence of Na+) by removal of HCO3-/CO2 or addition of 4-acetamido-4-isothiocyanatostilbene-2,2-disulfonic acid resulted in uncontrolled acidification of pHi despite increase in aiNa. Bicarbonates 23-27 glucose-6-phosphate isomerase Homo sapiens 202-205 7698616-7 1995 Similarly, blocking of HCO3- transport (in the presence of Na+) by removal of HCO3-/CO2 or addition of 4-acetamido-4-isothiocyanatostilbene-2,2-disulfonic acid resulted in uncontrolled acidification of pHi despite increase in aiNa. 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 103-159 glucose-6-phosphate isomerase Homo sapiens 202-205 7698616-9 1995 CONCLUSIONS: The data indicate that during short-term exposure of the gastric mucosa to luminal acid, both Na+/H+ antiport and HCO3- transport are needed to control pHi and maintain it within physiological ranges. Bicarbonates 127-131 glucose-6-phosphate isomerase Homo sapiens 165-168 7656191-6 1995 When pHo was kept at 7.40 during hypercapnia by increasing sodium bicarbonate concentration, DT recovered to 79.11 +/- 6.94% of control after 30 mins of hypercapnia, while a significant recovery of pHi (0.12 +/- 0.02 pH units) was detected. Sodium Bicarbonate 59-77 glucose-6-phosphate isomerase Homo sapiens 198-201 7494140-5 1995 The currents mirrored the changes in pHi, were strictly dependent on the presence of a Na+ gradient and were reversibly blocked by amiloride. Amiloride 131-140 glucose-6-phosphate isomerase Homo sapiens 37-40 8544928-3 1995 In patients with different forms of acute cerebrovascular damage it was found that there was hyperglycaemia in the blood of unfed patients, elevated blood fructose level and increased serum PHI activity; those changes were increased by intravenous glucose administration. Glucose 248-255 glucose-6-phosphate isomerase Homo sapiens 190-193 7665751-1 1995 OBJECTIVE: To investigate whether infusing prostacyclin (PGI2) in patients with septic shock improves splanchnic oxygenation as assessed by gastric intramucosal pH (pHi). Epoprostenol 43-55 glucose-6-phosphate isomerase Homo sapiens 165-168 7665751-10 1995 While O2 uptake remained unchanged, infusing PGI2 increased O2 delivery (from 610 +/- 140 to 682 +/- 155 ml/min.m2, p < 0.01) and pHi (from 7.32 +/- 0.09 to 7.38 +/- 0.08, p < 0.001) beyond the values obtained by conventional resuscitation. Epoprostenol 45-49 glucose-6-phosphate isomerase Homo sapiens 133-136 7665751-12 1995 CONCLUSIONS: Infusing PGI2 in patients with septic shock increases pHi probably by enhancing blood flow to the splanchnic bed and thereby improves splanchnic oxygenation even when conventional resuscitation goals have been achieved. Epoprostenol 22-26 glucose-6-phosphate isomerase Homo sapiens 67-70 7778066-6 1995 The pHi-stimulating effect of endothelin-1 was inhibited by 10(-7) M staurosporine, calphostin C and 5-(N,N-hexamethylene) amiloride. calphostin C 84-96 glucose-6-phosphate isomerase Homo sapiens 4-7 7778066-6 1995 The pHi-stimulating effect of endothelin-1 was inhibited by 10(-7) M staurosporine, calphostin C and 5-(N,N-hexamethylene) amiloride. 5-(N,N-hexamethylene)amiloride 101-132 glucose-6-phosphate isomerase Homo sapiens 4-7 7778066-7 1995 BQ-123 (10(-7) M) abolished the pHi responses to endothelin-1, whereas sarafotoxin S6c had no effect on platelet pHi. cyclo(Trp-Asp-Pro-Val-Leu) 0-6 glucose-6-phosphate isomerase Homo sapiens 32-35 19877915-9 1995 ATP modulation of pH(i) may be coupled to the rise in [Ca(2+)](j) known to occur with ATP stimulation. Adenosine Triphosphate 0-3 glucose-6-phosphate isomerase Homo sapiens 18-23 19877915-9 1995 ATP modulation of pH(i) may be coupled to the rise in [Ca(2+)](j) known to occur with ATP stimulation. Adenosine Triphosphate 86-89 glucose-6-phosphate isomerase Homo sapiens 18-23 7900765-9 1995 In HEPES, stimulation with K+, NE, ANG II, or AVP led to a fall in pHi, but this did not occur with ET-1. HEPES 3-8 glucose-6-phosphate isomerase Homo sapiens 67-70 7604180-7 1995 This basolateral Na(+)-dependent pHi recovery in the presence of HCO3-/CO2 was reduced, but present, in experiments where dimethylamiloride (DMA, 100 microM) or the stilbene derivative DIDS (500 microM) was in basolateral fluid. Bicarbonates 65-69 glucose-6-phosphate isomerase Homo sapiens 33-36 7698312-5 1995 By contrast, blockade of Na+ channels by 1-3 microM TTX prevented the effect of high K+ concentrations on pHi. Tetrodotoxin 52-55 glucose-6-phosphate isomerase Homo sapiens 106-109 7880851-4 1995 Continuous monitoring of intracellular pH (pHi) in BCECF (2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein)-loaded Caco-2 cell monolayers indicated that apical addition of MeAIB (20 mM) was associated with H(+)-flow across the apical membrane in both Na+ and Na(+)-free conditions. bcecf 51-56 glucose-6-phosphate isomerase Homo sapiens 43-46 7880851-4 1995 Continuous monitoring of intracellular pH (pHi) in BCECF (2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein)-loaded Caco-2 cell monolayers indicated that apical addition of MeAIB (20 mM) was associated with H(+)-flow across the apical membrane in both Na+ and Na(+)-free conditions. CACO 116-120 glucose-6-phosphate isomerase Homo sapiens 43-46 7880851-6 1995 On the basis of competition for MeAIB accumulation and pHi experiments, L-proline, glycine, L-alanine and beta-alanine are also substrates for H(+)-linked transport at the apical membrane of Caco-2 cells but L-valine, L-leucine and L-phenylalanine are not. beta-Alanine 106-118 glucose-6-phosphate isomerase Homo sapiens 55-58 7604180-7 1995 This basolateral Na(+)-dependent pHi recovery in the presence of HCO3-/CO2 was reduced, but present, in experiments where dimethylamiloride (DMA, 100 microM) or the stilbene derivative DIDS (500 microM) was in basolateral fluid. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 71-74 glucose-6-phosphate isomerase Homo sapiens 33-36 7604180-7 1995 This basolateral Na(+)-dependent pHi recovery in the presence of HCO3-/CO2 was reduced, but present, in experiments where dimethylamiloride (DMA, 100 microM) or the stilbene derivative DIDS (500 microM) was in basolateral fluid. 5-dimethylamiloride 122-139 glucose-6-phosphate isomerase Homo sapiens 33-36 7604180-7 1995 This basolateral Na(+)-dependent pHi recovery in the presence of HCO3-/CO2 was reduced, but present, in experiments where dimethylamiloride (DMA, 100 microM) or the stilbene derivative DIDS (500 microM) was in basolateral fluid. 5-dimethylamiloride 141-144 glucose-6-phosphate isomerase Homo sapiens 33-36 7604180-7 1995 This basolateral Na(+)-dependent pHi recovery in the presence of HCO3-/CO2 was reduced, but present, in experiments where dimethylamiloride (DMA, 100 microM) or the stilbene derivative DIDS (500 microM) was in basolateral fluid. Stilbenes 165-173 glucose-6-phosphate isomerase Homo sapiens 33-36 7604180-7 1995 This basolateral Na(+)-dependent pHi recovery in the presence of HCO3-/CO2 was reduced, but present, in experiments where dimethylamiloride (DMA, 100 microM) or the stilbene derivative DIDS (500 microM) was in basolateral fluid. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 185-189 glucose-6-phosphate isomerase Homo sapiens 33-36 7604180-10 1995 These data indicate that alveolar epithelial cells express a basolateral Na(+)- and HCO3(-)-dependent, DIDS-sensitive, Cl(-)-independent pHi recovery process that probably represents Na(+)-HCO3(-)-cotransport (symport). Bicarbonates 84-88 glucose-6-phosphate isomerase Homo sapiens 137-140 7604180-10 1995 These data indicate that alveolar epithelial cells express a basolateral Na(+)- and HCO3(-)-dependent, DIDS-sensitive, Cl(-)-independent pHi recovery process that probably represents Na(+)-HCO3(-)-cotransport (symport). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 103-107 glucose-6-phosphate isomerase Homo sapiens 137-140 7604180-10 1995 These data indicate that alveolar epithelial cells express a basolateral Na(+)- and HCO3(-)-dependent, DIDS-sensitive, Cl(-)-independent pHi recovery process that probably represents Na(+)-HCO3(-)-cotransport (symport). Bicarbonates 189-193 glucose-6-phosphate isomerase Homo sapiens 137-140 7900765-3 1995 pHi recovery from an acute acid load was dependent on external Na+ and partially inhibited by the absence of HCO3(-) [N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solution] or by the anion transport inhibitor 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS). Bicarbonates 109-113 glucose-6-phosphate isomerase Homo sapiens 0-3 7900765-3 1995 pHi recovery from an acute acid load was dependent on external Na+ and partially inhibited by the absence of HCO3(-) [N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solution] or by the anion transport inhibitor 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS). HEPES 118-169 glucose-6-phosphate isomerase Homo sapiens 0-3 7900765-3 1995 pHi recovery from an acute acid load was dependent on external Na+ and partially inhibited by the absence of HCO3(-) [N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solution] or by the anion transport inhibitor 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS). HEPES 171-176 glucose-6-phosphate isomerase Homo sapiens 0-3 7900765-3 1995 pHi recovery from an acute acid load was dependent on external Na+ and partially inhibited by the absence of HCO3(-) [N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solution] or by the anion transport inhibitor 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 233-281 glucose-6-phosphate isomerase Homo sapiens 0-3 7900765-3 1995 pHi recovery from an acute acid load was dependent on external Na+ and partially inhibited by the absence of HCO3(-) [N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solution] or by the anion transport inhibitor 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 283-287 glucose-6-phosphate isomerase Homo sapiens 0-3 7900765-4 1995 In an HCO3(-)-buffered physiological salt solution (PSS), pHi recovery was partially blocked by hexamethylene amiloride (HMA), an inhibitor of Na+/H+ exchange, and completely blocked by DIDS and HMA together. Bicarbonates 6-13 glucose-6-phosphate isomerase Homo sapiens 58-61 7900765-4 1995 In an HCO3(-)-buffered physiological salt solution (PSS), pHi recovery was partially blocked by hexamethylene amiloride (HMA), an inhibitor of Na+/H+ exchange, and completely blocked by DIDS and HMA together. salt solution 37-50 glucose-6-phosphate isomerase Homo sapiens 58-61 7900765-4 1995 In an HCO3(-)-buffered physiological salt solution (PSS), pHi recovery was partially blocked by hexamethylene amiloride (HMA), an inhibitor of Na+/H+ exchange, and completely blocked by DIDS and HMA together. pss 52-55 glucose-6-phosphate isomerase Homo sapiens 58-61 7900765-4 1995 In an HCO3(-)-buffered physiological salt solution (PSS), pHi recovery was partially blocked by hexamethylene amiloride (HMA), an inhibitor of Na+/H+ exchange, and completely blocked by DIDS and HMA together. 5-(N,N-hexamethylene)amiloride 96-119 glucose-6-phosphate isomerase Homo sapiens 58-61 7900765-4 1995 In an HCO3(-)-buffered physiological salt solution (PSS), pHi recovery was partially blocked by hexamethylene amiloride (HMA), an inhibitor of Na+/H+ exchange, and completely blocked by DIDS and HMA together. 5-(N,N-hexamethylene)amiloride 121-124 glucose-6-phosphate isomerase Homo sapiens 58-61 7736491-1 1995 The Na+/H+ exchanger in vascular smooth muscle cells represents a major mechanism for sodium influx and is also one of the principal mechanisms responsible for the regulation of intracellular pH (pHi). Sodium 86-92 glucose-6-phosphate isomerase Homo sapiens 196-199 7900765-4 1995 In an HCO3(-)-buffered physiological salt solution (PSS), pHi recovery was partially blocked by hexamethylene amiloride (HMA), an inhibitor of Na+/H+ exchange, and completely blocked by DIDS and HMA together. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 186-190 glucose-6-phosphate isomerase Homo sapiens 58-61 7900765-4 1995 In an HCO3(-)-buffered physiological salt solution (PSS), pHi recovery was partially blocked by hexamethylene amiloride (HMA), an inhibitor of Na+/H+ exchange, and completely blocked by DIDS and HMA together. 5-(N,N-hexamethylene)amiloride 195-198 glucose-6-phosphate isomerase Homo sapiens 58-61 7900765-6 1995 pHi recovery from acute alkalosis was unaffected by external Na+ removal, reduced in HEPES buffer, and abolished by removal of external Cl-. HEPES 85-90 glucose-6-phosphate isomerase Homo sapiens 0-3 7900765-7 1995 These data suggest that human small arteries maintain pHi by Na+/H+ exchange and Na(+)-dependent HCO3(-) exchange in response to an acid load, and Na(+)-independent Cl-/HCO3(-) exchange to counteract intracellular alkalosis. Bicarbonates 97-101 glucose-6-phosphate isomerase Homo sapiens 54-57 7538610-3 1995 This is supported by the fact that 97% of the change in macula densa pHi with reduction in [Cl]1 was bumetanide-sensitive whereas 92% of this pH change was amiloride-sensitive. Bumetanide 101-111 glucose-6-phosphate isomerase Homo sapiens 69-72 7736501-1 1995 OBJECTIVES: The aim was to determine the mechanisms, particularly bicarbonate dependent mechanisms, of intracellular pH (pHi) recovery from various acidoses in vascular smooth muscle and to explore the ATP dependency of the respective mechanisms. Bicarbonates 66-77 glucose-6-phosphate isomerase Homo sapiens 121-124 7736501-10 1995 Under such conditions HCO3- dependent mechanisms contributed about 40% to the overall pHi regulating capacity of vascular smooth muscle cells. Bicarbonates 22-26 glucose-6-phosphate isomerase Homo sapiens 86-89 7736501-11 1995 However, under conditions which deplete cellular ATP these pHi regulating mechanisms account for virtually all of theses cells" ability to regulate pHi. Adenosine Triphosphate 49-52 glucose-6-phosphate isomerase Homo sapiens 59-62 7736501-11 1995 However, under conditions which deplete cellular ATP these pHi regulating mechanisms account for virtually all of theses cells" ability to regulate pHi. Adenosine Triphosphate 49-52 glucose-6-phosphate isomerase Homo sapiens 148-151 7736501-17 1995 When the Na+/H+ exchanger is attenuated by cellular ATP depletion, the alternative pathways, particularly the Na(+)-HCO3- cotransporter, ensure that substantial pHi regulatory capacity is maintained. Adenosine Triphosphate 52-55 glucose-6-phosphate isomerase Homo sapiens 161-164 7739095-11 1995 With infusions of GL and large dose of PGE1, pHi tended to decrease further, although GL increased the cardiac output. Glucagon 18-20 glucose-6-phosphate isomerase Homo sapiens 45-48 7739095-11 1995 With infusions of GL and large dose of PGE1, pHi tended to decrease further, although GL increased the cardiac output. Alprostadil 39-43 glucose-6-phosphate isomerase Homo sapiens 45-48 7739095-13 1995 PF treatment showed beneficial effects not only on the cardiac output and the SMA blood flow, but also on pHi. Pentoxifylline 0-2 glucose-6-phosphate isomerase Homo sapiens 106-109 7731032-5 1995 From these experiments, we observed that under some circumstances Vm varied with pHo but without Gm or pHi changes, whereas under other circumstances changes of Gm occurred during alterations of pHi while pHo and Vm remained unaltered. gm 161-163 glucose-6-phosphate isomerase Homo sapiens 195-198 7880127-7 1995 Another five of the 20 showed low GPI dependency aCL antibodies; however, three of these patients required GPI for binding to phosphatidylserine, phosphatidylinositol, or both. Phosphatidylserines 126-144 glucose-6-phosphate isomerase Homo sapiens 107-110 7880127-7 1995 Another five of the 20 showed low GPI dependency aCL antibodies; however, three of these patients required GPI for binding to phosphatidylserine, phosphatidylinositol, or both. Phosphatidylinositols 146-166 glucose-6-phosphate isomerase Homo sapiens 107-110 10155169-7 1995 Further, a low gastric mucosal pHi on admission to the ICU appears to be predictive of mortality and pHi-guided resuscitation may improve outcome in a subpopulation of patients admitted to the ICU with normal pHi, perhaps by preventing splanchnic ischemia and the development of a systemic oxygen deficit. Oxygen 290-296 glucose-6-phosphate isomerase Homo sapiens 31-34 10155169-7 1995 Further, a low gastric mucosal pHi on admission to the ICU appears to be predictive of mortality and pHi-guided resuscitation may improve outcome in a subpopulation of patients admitted to the ICU with normal pHi, perhaps by preventing splanchnic ischemia and the development of a systemic oxygen deficit. Oxygen 290-296 glucose-6-phosphate isomerase Homo sapiens 101-104 10155169-7 1995 Further, a low gastric mucosal pHi on admission to the ICU appears to be predictive of mortality and pHi-guided resuscitation may improve outcome in a subpopulation of patients admitted to the ICU with normal pHi, perhaps by preventing splanchnic ischemia and the development of a systemic oxygen deficit. Oxygen 290-296 glucose-6-phosphate isomerase Homo sapiens 101-104 7872361-3 1995 pHi was measured, using the fluorescent probe BCECF (2",7"-bis-carboxyethyl-5,6-carboxyfluorescein), both in nominal absence of bicarbonate (Hepes solution, pH 7.4; n = 10) and in the presence of HCO3-/CO2 buffer system (pH 7.4, [HCO3-] 25 mM, pCO2 40 mm Hg; n = 6). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 46-51 glucose-6-phosphate isomerase Homo sapiens 0-3 7872361-3 1995 pHi was measured, using the fluorescent probe BCECF (2",7"-bis-carboxyethyl-5,6-carboxyfluorescein), both in nominal absence of bicarbonate (Hepes solution, pH 7.4; n = 10) and in the presence of HCO3-/CO2 buffer system (pH 7.4, [HCO3-] 25 mM, pCO2 40 mm Hg; n = 6). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 53-98 glucose-6-phosphate isomerase Homo sapiens 0-3 7872361-3 1995 pHi was measured, using the fluorescent probe BCECF (2",7"-bis-carboxyethyl-5,6-carboxyfluorescein), both in nominal absence of bicarbonate (Hepes solution, pH 7.4; n = 10) and in the presence of HCO3-/CO2 buffer system (pH 7.4, [HCO3-] 25 mM, pCO2 40 mm Hg; n = 6). Bicarbonates 196-200 glucose-6-phosphate isomerase Homo sapiens 0-3 7872361-3 1995 pHi was measured, using the fluorescent probe BCECF (2",7"-bis-carboxyethyl-5,6-carboxyfluorescein), both in nominal absence of bicarbonate (Hepes solution, pH 7.4; n = 10) and in the presence of HCO3-/CO2 buffer system (pH 7.4, [HCO3-] 25 mM, pCO2 40 mm Hg; n = 6). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 202-205 glucose-6-phosphate isomerase Homo sapiens 0-3 7872361-3 1995 pHi was measured, using the fluorescent probe BCECF (2",7"-bis-carboxyethyl-5,6-carboxyfluorescein), both in nominal absence of bicarbonate (Hepes solution, pH 7.4; n = 10) and in the presence of HCO3-/CO2 buffer system (pH 7.4, [HCO3-] 25 mM, pCO2 40 mm Hg; n = 6). Bicarbonates 230-234 glucose-6-phosphate isomerase Homo sapiens 0-3 7872361-3 1995 pHi was measured, using the fluorescent probe BCECF (2",7"-bis-carboxyethyl-5,6-carboxyfluorescein), both in nominal absence of bicarbonate (Hepes solution, pH 7.4; n = 10) and in the presence of HCO3-/CO2 buffer system (pH 7.4, [HCO3-] 25 mM, pCO2 40 mm Hg; n = 6). pco2 244-248 glucose-6-phosphate isomerase Homo sapiens 0-3 7872361-4 1995 Predialysis pHi did not differ from controls when measured in the presence of HCO3-/CO2 (7.28 +/- 0.04 vs. 7.29 +/- 0.04, p = NS), but was lower in dialysis patients than in normal subjects (7.11 +/- 0.04 and 7.20 +/- 0.02, respectively; p < 0.05) when measured in Hepes solution. HEPES 268-273 glucose-6-phosphate isomerase Homo sapiens 12-15 7872361-5 1995 This suggested that bicarbonate-independent pHi regulation was abnormal in dialysis patients. Bicarbonates 20-31 glucose-6-phosphate isomerase Homo sapiens 44-47 7872361-6 1995 To further characterize this abnormality of pHi regulation, lymphocytes were exposed to ethylisopropylamiloride, a specific Na+/H+ antiporter inhibitor, in Hepes solution; this maneuver induced a significantly lower decrement in pHi (0.04 +/- 0.04 vs. 0.15 +/- 0.03, p < 0.05) in dialysis patients than in controls, indicating reduced Na+/H+ exchanger activity in the patients. ethylisopropylamiloride 88-111 glucose-6-phosphate isomerase Homo sapiens 44-47 7872361-6 1995 To further characterize this abnormality of pHi regulation, lymphocytes were exposed to ethylisopropylamiloride, a specific Na+/H+ antiporter inhibitor, in Hepes solution; this maneuver induced a significantly lower decrement in pHi (0.04 +/- 0.04 vs. 0.15 +/- 0.03, p < 0.05) in dialysis patients than in controls, indicating reduced Na+/H+ exchanger activity in the patients. ethylisopropylamiloride 88-111 glucose-6-phosphate isomerase Homo sapiens 229-232 7872361-6 1995 To further characterize this abnormality of pHi regulation, lymphocytes were exposed to ethylisopropylamiloride, a specific Na+/H+ antiporter inhibitor, in Hepes solution; this maneuver induced a significantly lower decrement in pHi (0.04 +/- 0.04 vs. 0.15 +/- 0.03, p < 0.05) in dialysis patients than in controls, indicating reduced Na+/H+ exchanger activity in the patients. HEPES 156-161 glucose-6-phosphate isomerase Homo sapiens 44-47 7737089-3 1995 A 0.5 M Tris-HCl, pH 7.5, gel buffer was optimal for gels stained for the isozymes of 6-PGD, PGI and SkDH. Tris hydrochloride 8-16 glucose-6-phosphate isomerase Homo sapiens 93-96 7731032-6 1995 At pHi approximately 6.5 associated with Vm approximately -10 mV, Gm dramatically increased to quasi-infinite values. gm 66-68 glucose-6-phosphate isomerase Homo sapiens 3-6 7731032-8 1995 In conclusion, we demonstrate a differential regulation whereby Vm and Gm are controlled by pHo and pHi: pHo modulates mainly Vm and pHi modulates chiefly Gm. gm 71-73 glucose-6-phosphate isomerase Homo sapiens 100-103 7731032-8 1995 In conclusion, we demonstrate a differential regulation whereby Vm and Gm are controlled by pHo and pHi: pHo modulates mainly Vm and pHi modulates chiefly Gm. gm 71-73 glucose-6-phosphate isomerase Homo sapiens 133-136 7731032-8 1995 In conclusion, we demonstrate a differential regulation whereby Vm and Gm are controlled by pHo and pHi: pHo modulates mainly Vm and pHi modulates chiefly Gm. gm 155-157 glucose-6-phosphate isomerase Homo sapiens 100-103 7731032-8 1995 In conclusion, we demonstrate a differential regulation whereby Vm and Gm are controlled by pHo and pHi: pHo modulates mainly Vm and pHi modulates chiefly Gm. gm 155-157 glucose-6-phosphate isomerase Homo sapiens 133-136 7731032-9 1995 Furthermore, at pHi approximately 6.5 and Vm approximately -10 mV, our data reveal a large Gm that tends towards infinite values in a reversible fashion. gm 91-93 glucose-6-phosphate isomerase Homo sapiens 16-19 7760347-2 1995 Developed tension (DT), intracellular pH (pHi) with the pH-sensitive dye 2"-7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) and resting membrane potential (Vm) with 3M KCl filled glass microelectrodes were measured. bcecf 123-128 glucose-6-phosphate isomerase Homo sapiens 42-45 7760347-3 1995 A change from HEPES to HCO3(-)-buffered superfusate induced an immediate decrease in pHi and DT followed by a recovery in which pHi and DT stabilized at values slightly higher than in HEPES buffer. HEPES 14-19 glucose-6-phosphate isomerase Homo sapiens 85-88 7760347-3 1995 A change from HEPES to HCO3(-)-buffered superfusate induced an immediate decrease in pHi and DT followed by a recovery in which pHi and DT stabilized at values slightly higher than in HEPES buffer. HEPES 14-19 glucose-6-phosphate isomerase Homo sapiens 128-131 7760347-3 1995 A change from HEPES to HCO3(-)-buffered superfusate induced an immediate decrease in pHi and DT followed by a recovery in which pHi and DT stabilized at values slightly higher than in HEPES buffer. Bicarbonates 23-30 glucose-6-phosphate isomerase Homo sapiens 85-88 7760347-3 1995 A change from HEPES to HCO3(-)-buffered superfusate induced an immediate decrease in pHi and DT followed by a recovery in which pHi and DT stabilized at values slightly higher than in HEPES buffer. Bicarbonates 23-30 glucose-6-phosphate isomerase Homo sapiens 128-131 7760347-11 1995 However, acid equivalent extrusion is potentiated when both the Na+/H+ exchanger and the HCO3-dependent mechanism are simultaneously regulating pHi. Bicarbonates 89-93 glucose-6-phosphate isomerase Homo sapiens 144-147 7933396-8 1994 Although oxygen delivery and oxygen consumption increased in both groups of patients, the pHi increased significantly in those patients treated with norepinephrine whereas the pHi decreased significantly in those patients receiving dopamine (P < .001, for corrected 3-hour value). Norepinephrine 149-163 glucose-6-phosphate isomerase Homo sapiens 90-93 7774123-7 1994 GPi activity of monkey rendered Parkinsonism by MPTP is abnormally high and activity of GPe is lower than that before MPTP application, while the GPi activity of STN-lesioned monkey showing limb dyskinesia (hemiballismus) is much lower than normal animal. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine 48-52 glucose-6-phosphate isomerase Homo sapiens 0-3 7955153-3 1994 pHi was altered at constant pHo by use of NH4Cl and measured with the fluorescent dye BCECF. Ammonium Chloride 42-47 glucose-6-phosphate isomerase Homo sapiens 0-3 7955153-3 1994 pHi was altered at constant pHo by use of NH4Cl and measured with the fluorescent dye BCECF. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 86-91 glucose-6-phosphate isomerase Homo sapiens 0-3 7955153-8 1994 Addition of NH4Cl elicited a rapid monotonic increase in pHi and then a slow recovery toward initial levels; washout of NH4Cl led to a rapid acidification followed by recovery. Ammonium Chloride 12-17 glucose-6-phosphate isomerase Homo sapiens 57-60 7955153-12 1994 Thus, the effects on force of the NH4Cl-induced changes in pHi are associated with changes in the Ca2+ sensitivity of the contractile apparatus rather than mediated through changes in [Ca2+]i. Ammonium Chloride 34-39 glucose-6-phosphate isomerase Homo sapiens 59-62 7525616-13 1994 Moreover, activation of protein kinase C by the addition of phorbol esters increased the pHi in cells plated on cytotactin/tenascin or counteradhesive fusion proteins and reversed their effects. Phorbol Esters 60-74 glucose-6-phosphate isomerase Homo sapiens 89-92 7933396-8 1994 Although oxygen delivery and oxygen consumption increased in both groups of patients, the pHi increased significantly in those patients treated with norepinephrine whereas the pHi decreased significantly in those patients receiving dopamine (P < .001, for corrected 3-hour value). Dopamine 232-240 glucose-6-phosphate isomerase Homo sapiens 176-179 7823033-6 1994 We observed the expected pHi changes when we exposed the basolateral (i.e. blood-side) membrane to a pH 6.4 solution (a large, rapid pHi decrease), to a pH 7.4 solution containing approximately 0.3 mmoll-1 NH3 (a large and rapid pHi increase) or to a pH7.4 solution equilibrated with 1% CO2 (a rapid pHi decrease of -0.08). Ammonia 206-209 glucose-6-phosphate isomerase Homo sapiens 25-28 7525551-7 1994 Using fluorimetric determinations of pHi, a conductive, Zn(2+)-sensitive alkalinization was observed in neutrophils from both normal and cytochrome b-deficient CGD donors. Zinc 56-62 glucose-6-phosphate isomerase Homo sapiens 37-40 7823033-6 1994 We observed the expected pHi changes when we exposed the basolateral (i.e. blood-side) membrane to a pH 6.4 solution (a large, rapid pHi decrease), to a pH 7.4 solution containing approximately 0.3 mmoll-1 NH3 (a large and rapid pHi increase) or to a pH7.4 solution equilibrated with 1% CO2 (a rapid pHi decrease of -0.08). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 287-290 glucose-6-phosphate isomerase Homo sapiens 25-28 7838685-10 1994 These results suggest that reduced PO2 increases the activity of a high-conductance, Ca(2+)-sensitive K+ channel in cat cerebral arterial muscle cells, and that these effects are mediated by cytosolic events independent of changes in [Ca2+]i and pHi. PO-2 35-38 glucose-6-phosphate isomerase Homo sapiens 246-249 7943268-3 1994 However, after 30 min of RVD, pHi is not significantly different from the initial pHi in 20 mM HCO3- medium and is significantly higher in HCO3(-)-free medium. Bicarbonates 95-99 glucose-6-phosphate isomerase Homo sapiens 30-33 7943268-3 1994 However, after 30 min of RVD, pHi is not significantly different from the initial pHi in 20 mM HCO3- medium and is significantly higher in HCO3(-)-free medium. Bicarbonates 139-143 glucose-6-phosphate isomerase Homo sapiens 30-33 7943268-6 1994 We found that inhibition of Na+/H+ exchange (NHE) with 12.5 microM ethylisoproplamiloride (EIPA) causes pHi to fall significantly by the end of 30 min of RVD. ethylisoproplamiloride 67-89 glucose-6-phosphate isomerase Homo sapiens 104-107 7943268-6 1994 We found that inhibition of Na+/H+ exchange (NHE) with 12.5 microM ethylisoproplamiloride (EIPA) causes pHi to fall significantly by the end of 30 min of RVD. ethylisopropylamiloride 91-95 glucose-6-phosphate isomerase Homo sapiens 104-107 7837098-4 1994 pHi was measured using the fluorescent probe 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 45-91 glucose-6-phosphate isomerase Homo sapiens 0-3 7837098-4 1994 pHi was measured using the fluorescent probe 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 93-98 glucose-6-phosphate isomerase Homo sapiens 0-3 7971175-2 1994 Under control conditions, intracellular pH (pHi) was 7.21 +/- 0.07 (n = 22) in HCO3(-)-containing and 7.21 +/- 0.09 (n = 12) in HCO3(-)-free solution. Bicarbonates 79-86 glucose-6-phosphate isomerase Homo sapiens 44-47 8083368-5 1994 Salt loading elevated Cai and Nai while suppressing Mgi and pHi; these changes occurred predominantly in salt-sensitive subjects (n = 9). Salts 0-4 glucose-6-phosphate isomerase Homo sapiens 60-63 8083368-6 1994 Nifedipine blunted the pressor response to salt loading > 50% (delta diastolic BP [high-low salt vs placebo] = 5 +/- 2 vs 14 +/- 2 mmHg, P < 0.05) and reversed salt-induced ionic changes, lowering Cai and elevating Mgi and pHi. Nifedipine 0-10 glucose-6-phosphate isomerase Homo sapiens 229-232 8083368-6 1994 Nifedipine blunted the pressor response to salt loading > 50% (delta diastolic BP [high-low salt vs placebo] = 5 +/- 2 vs 14 +/- 2 mmHg, P < 0.05) and reversed salt-induced ionic changes, lowering Cai and elevating Mgi and pHi. Salts 43-47 glucose-6-phosphate isomerase Homo sapiens 229-232 8083368-7 1994 Regardless of the definition of salt sensitivity, continuous relationships were observed between the pressure response to salt loading, the levels of Cai (r = 0.726, P < 0.001), Nai (r = 0.747, P < 0.001), and pHi (r = -0.754, P < 0.001), and the salt-induced change in Mgi (r = -0.757, P < 0.001). Salts 122-126 glucose-6-phosphate isomerase Homo sapiens 216-219 8083368-7 1994 Regardless of the definition of salt sensitivity, continuous relationships were observed between the pressure response to salt loading, the levels of Cai (r = 0.726, P < 0.001), Nai (r = 0.747, P < 0.001), and pHi (r = -0.754, P < 0.001), and the salt-induced change in Mgi (r = -0.757, P < 0.001). Salts 122-126 glucose-6-phosphate isomerase Homo sapiens 216-219 8051419-9 1994 Tenidap did not alter pHi via inhibition of the sodium-proton antiporter, but inhibited activity of chloride-bicarbonate exchangers, as did UK5099, a known anion-transport inhibitor that also lowers pHi. 2-cyano-3-(1-phenylindol-3-yl)acrylate 140-146 glucose-6-phosphate isomerase Homo sapiens 199-202 8051419-6 1994 In cells loaded with the pH-sensitive fluorescence dye 2",7"-bis-(2-carboxyethyl)-5-(and -6) carboxyfluorescein, tenidap, but not NSAIDs, caused a rapid and sustained acidification of the cytoplasmic compartment. 2",7"-bis-(2-carboxyethyl)-5-(and -6) carboxyfluorescein 55-111 glucose-6-phosphate isomerase Homo sapiens 25-27 8051419-8 1994 Mammalian cells regulate pHi through the concerted action of a number of specific transport proteins, including sodium-proton antiporters and chloride-bicarbonate exchangers. Chlorides 142-150 glucose-6-phosphate isomerase Homo sapiens 25-28 8051419-8 1994 Mammalian cells regulate pHi through the concerted action of a number of specific transport proteins, including sodium-proton antiporters and chloride-bicarbonate exchangers. Bicarbonates 151-162 glucose-6-phosphate isomerase Homo sapiens 25-28 8049810-2 1994 In the present study we measured changes in gastric pHi with a tonometer in septic patients after a short-term infusion of dobutamine. Dobutamine 123-133 glucose-6-phosphate isomerase Homo sapiens 52-55 8049810-10 1994 Gastric pHi increased in both groups when dobutamine was infused at 5 micrograms/kg/min (p < 0.01), and then again at 10 micrograms/kg/min (p < 0.05). Dobutamine 42-52 glucose-6-phosphate isomerase Homo sapiens 8-11 8074171-3 1994 Basal pHi averaged approximately 7.2 in HCO3- media and 0.1-0.15 pH units lower in nominally HCO3(-)-free media in all cell types. Bicarbonates 40-44 glucose-6-phosphate isomerase Homo sapiens 6-9 8049810-12 1994 Moreover, a rise in gastric pHi in response to increases in systemic O2 transport may be a better indicator of regional hypoxia in septic patients than related increases in systemic VO2. Oxygen 69-71 glucose-6-phosphate isomerase Homo sapiens 28-31 8074171-3 1994 Basal pHi averaged approximately 7.2 in HCO3- media and 0.1-0.15 pH units lower in nominally HCO3(-)-free media in all cell types. Bicarbonates 40-44 glucose-6-phosphate isomerase Homo sapiens 6-8 8049810-12 1994 Moreover, a rise in gastric pHi in response to increases in systemic O2 transport may be a better indicator of regional hypoxia in septic patients than related increases in systemic VO2. vo2 182-185 glucose-6-phosphate isomerase Homo sapiens 28-31 8020795-1 1994 A high intragastric PCO2 (iPCO2), determined tonometrically, is the main factor participating in a low gastric intramucosal pH (pHi) and may point to gastric mucosal ischaemia. pco2 20-24 glucose-6-phosphate isomerase Homo sapiens 128-131 7966013-8 1994 The intracellular pH (pHi) of intact spermatozoa, estimated by measuring the accumulation of 9-aminoacridine fluorescence, increased with increasing pHe at both 30 degrees C and 40 degrees C. These results demonstrate that the reversible temperature-dependent immobilization of fowl spermatozoa at 40 degrees C is inhibited by an increased pHi. Aminacrine 93-108 glucose-6-phosphate isomerase Homo sapiens 22-25 7966013-8 1994 The intracellular pH (pHi) of intact spermatozoa, estimated by measuring the accumulation of 9-aminoacridine fluorescence, increased with increasing pHe at both 30 degrees C and 40 degrees C. These results demonstrate that the reversible temperature-dependent immobilization of fowl spermatozoa at 40 degrees C is inhibited by an increased pHi. Phenylalanine 149-152 glucose-6-phosphate isomerase Homo sapiens 22-25 7966013-8 1994 The intracellular pH (pHi) of intact spermatozoa, estimated by measuring the accumulation of 9-aminoacridine fluorescence, increased with increasing pHe at both 30 degrees C and 40 degrees C. These results demonstrate that the reversible temperature-dependent immobilization of fowl spermatozoa at 40 degrees C is inhibited by an increased pHi. Phenylalanine 149-152 glucose-6-phosphate isomerase Homo sapiens 340-343 8020795-1 1994 A high intragastric PCO2 (iPCO2), determined tonometrically, is the main factor participating in a low gastric intramucosal pH (pHi) and may point to gastric mucosal ischaemia. ipco2 26-31 glucose-6-phosphate isomerase Homo sapiens 128-131 7946993-1 1994 The effect of extracellular pH (pHe) on intracellular pH (pHi) and cellular metabolism was examined by multinuclear NMR spectroscopy of cells in vivo and in vitro. Phenylalanine 32-35 glucose-6-phosphate isomerase Homo sapiens 58-61 8089832-6 1994 The increment in pHi was blunted by the amiloride derivative EIPA, indicating that it was mediated by the Na+/H+ exchanger. Amiloride 40-49 glucose-6-phosphate isomerase Homo sapiens 17-20 8089832-6 1994 The increment in pHi was blunted by the amiloride derivative EIPA, indicating that it was mediated by the Na+/H+ exchanger. ethylisopropylamiloride 61-65 glucose-6-phosphate isomerase Homo sapiens 17-20 7914467-0 1994 The influence of pH on glutamate- and depolarization-induced increases of intracellular calcium concentration in cortical neurons in primary culture. Glutamic Acid 23-32 glucose-6-phosphate isomerase Homo sapiens 17-19 7914467-0 1994 The influence of pH on glutamate- and depolarization-induced increases of intracellular calcium concentration in cortical neurons in primary culture. Calcium 88-95 glucose-6-phosphate isomerase Homo sapiens 17-19 7914467-2 1994 Although a reduction in pHe/pHi per se increased [Ca2+]i, the acidosis attenuated both the peak rise in [Ca2+]i following exposure to glutamate, and the plateau level observed during prolonged exposure. Glutamic Acid 134-143 glucose-6-phosphate isomerase Homo sapiens 28-31 7914467-3 1994 As a result, cells exposed to solutions with low pH consistently had lower [Ca2+]i values upon glutamate exposure than cells studied at normal pH. Glutamic Acid 95-104 glucose-6-phosphate isomerase Homo sapiens 49-51 7914467-4 1994 Alkalosis, i.e., an increase in pHe/pHi, had the opposite effect, accentuating the glutamate-induced [Ca2+]i transients. Glutamic Acid 83-92 glucose-6-phosphate isomerase Homo sapiens 36-39 7914467-5 1994 Experiments designed to separate changes due to extra- and intracellular pH suggested that the decisive event was the change in pHe. Phenylalanine 128-131 glucose-6-phosphate isomerase Homo sapiens 73-75 7946993-2 1994 A decrease in pHe from 7.4 to 6.4 led to a significant drop in pHi, in both neuronal and glial tumour cells, as detected by in vivo 31P NMR of cells embedded in basement membrane gel threads. Phenylalanine 14-17 glucose-6-phosphate isomerase Homo sapiens 63-66 7946993-3 1994 A more than 50% decrease in both the phosphocreatine (PCr) level and derivatives of glycolysis (i.e., glycerol 3-phosphate) was observed, concomitantly to the fall in pHi. Phosphocreatine 37-52 glucose-6-phosphate isomerase Homo sapiens 167-170 7946993-3 1994 A more than 50% decrease in both the phosphocreatine (PCr) level and derivatives of glycolysis (i.e., glycerol 3-phosphate) was observed, concomitantly to the fall in pHi. Phosphocreatine 54-57 glucose-6-phosphate isomerase Homo sapiens 167-170 7946993-3 1994 A more than 50% decrease in both the phosphocreatine (PCr) level and derivatives of glycolysis (i.e., glycerol 3-phosphate) was observed, concomitantly to the fall in pHi. alpha-glycerophosphoric acid 102-122 glucose-6-phosphate isomerase Homo sapiens 167-170 7946993-5 1994 Reperfusion with fresh medium (pHe 7.4) resulted in the full recovery of pHi, simultaneously with an increase in both PCr and intracellular lactate back to their control levels. Phenylalanine 31-34 glucose-6-phosphate isomerase Homo sapiens 73-76 7946993-8 1994 pHi measurements in the presence of inhibitors of the various pH regulatory mechanisms showed that the Na+/H+ exchanger, the carbonic anhydrase and at least one of the bicarbonate-transport systems are involved in pH regulation of both cell types. Bicarbonates 168-179 glucose-6-phosphate isomerase Homo sapiens 0-3 8175807-3 1994 In wild-type transfectants, a short (up to 1 min) incubation with ionomycin induced a significant alkaline shift (approximately 0.2 pH unit) in the intracellular pH (pHi) dependence of the rate of 5-(N-ethyl-N-isopropyl) amiloride-sensitive 22Na+ uptake, without changes in the cell volume and phosphorylation state of NHE1. (n-ethyl-n-isopropyl) amiloride 199-230 glucose-6-phosphate isomerase Homo sapiens 166-169 8175807-3 1994 In wild-type transfectants, a short (up to 1 min) incubation with ionomycin induced a significant alkaline shift (approximately 0.2 pH unit) in the intracellular pH (pHi) dependence of the rate of 5-(N-ethyl-N-isopropyl) amiloride-sensitive 22Na+ uptake, without changes in the cell volume and phosphorylation state of NHE1. Ionomycin 66-75 glucose-6-phosphate isomerase Homo sapiens 166-169 8175807-3 1994 In wild-type transfectants, a short (up to 1 min) incubation with ionomycin induced a significant alkaline shift (approximately 0.2 pH unit) in the intracellular pH (pHi) dependence of the rate of 5-(N-ethyl-N-isopropyl) amiloride-sensitive 22Na+ uptake, without changes in the cell volume and phosphorylation state of NHE1. Sodium-22 241-246 glucose-6-phosphate isomerase Homo sapiens 166-169 8203534-5 1994 pHi was measured with quantitative fluorescence microscopy of cells loaded with the pH indicator, 2",7"-bis-(2-carboxyethyl)-5,6-carboxyfluorescein. 2",7"-bis-(2-carboxyethyl)-5,6-carboxyfluorescein 98-147 glucose-6-phosphate isomerase Homo sapiens 0-3 8203534-7 1994 After 60 min in CN(-)-fructose, pHi fell to 6.74 +/- 0.01 (n = 129, P < 0.001). Fructose 18-30 glucose-6-phosphate isomerase Homo sapiens 32-35 8203529-4 1994 Resting pHi in N-2-hydroxyethylpiperazine-N"-2- ethanesulfonic acid-buffered NaCl Ringer was 7.06 +/- 0.02. HEPES 15-67 glucose-6-phosphate isomerase Homo sapiens 8-11 8203534-8 1994 The pHi recovery rate (expressed as mmol H+.l-1.min-1) was determined under both conditions after acid loading by transient exposure and removal of 20 mM NH4Cl. Ammonium Chloride 154-159 glucose-6-phosphate isomerase Homo sapiens 4-7 8203529-4 1994 Resting pHi in N-2-hydroxyethylpiperazine-N"-2- ethanesulfonic acid-buffered NaCl Ringer was 7.06 +/- 0.02. Sodium Chloride 77-81 glucose-6-phosphate isomerase Homo sapiens 8-11 8141425-0 1994 A method for calculating the distribution of pH in tissues and a new source of pH error from the 31P-NMR spectrum. ET bromodomain inhibitor 97-100 glucose-6-phosphate isomerase Homo sapiens 79-81 8203529-5 1994 Removal of external Na+ caused reversible acidification; recovery of pHi from NH+4-induced acid load was Na+ dependent, amiloride inhibitable, and Cl-independent. Amiloride 120-129 glucose-6-phosphate isomerase Homo sapiens 69-72 8203529-6 1994 Asn and Gln had no measurable effect on resting pHi, but pretreatment with Asn or Gln induced a consistent twofold increase in pHi recovery from an acid challenge that was not seen with L-proline, D-glutamine, or L-phenylalanine. Asparagine 75-78 glucose-6-phosphate isomerase Homo sapiens 127-130 8203529-6 1994 Asn and Gln had no measurable effect on resting pHi, but pretreatment with Asn or Gln induced a consistent twofold increase in pHi recovery from an acid challenge that was not seen with L-proline, D-glutamine, or L-phenylalanine. Glutamine 82-85 glucose-6-phosphate isomerase Homo sapiens 127-130 8203529-6 1994 Asn and Gln had no measurable effect on resting pHi, but pretreatment with Asn or Gln induced a consistent twofold increase in pHi recovery from an acid challenge that was not seen with L-proline, D-glutamine, or L-phenylalanine. Phenylalanine 213-228 glucose-6-phosphate isomerase Homo sapiens 127-130 8203529-8 1994 The tumor promotor phorbol 12-myristate 13-acetate (PMA) stimulated recovery rate from acid load and also increased resting pHi. Tetradecanoylphorbol Acetate 19-50 glucose-6-phosphate isomerase Homo sapiens 124-127 8203529-8 1994 The tumor promotor phorbol 12-myristate 13-acetate (PMA) stimulated recovery rate from acid load and also increased resting pHi. Tetradecanoylphorbol Acetate 52-55 glucose-6-phosphate isomerase Homo sapiens 124-127 8051690-2 1994 Activation of Cl- as well as H(+)-selective currents may give rise to stimulus-induced changes in membrane potential and counteract changes in intracellular pH (pHi) which have been observed to be closely associated with respiratory burst activation and superoxide production in macrophages. Superoxides 254-264 glucose-6-phosphate isomerase Homo sapiens 161-164 8051690-9 1994 Activation of both conductances would contribute to the changes in membrane potential which accompany stimulation-induced activation of macrophages as well as counteract the decrease in pHi during sustained superoxide production. Superoxides 207-217 glucose-6-phosphate isomerase Homo sapiens 186-189 8178966-0 1994 pHi-dependent Cl-HCO3 exchange at the basolateral membrane of frog retinal pigment epithelium. Bicarbonates 17-21 glucose-6-phosphate isomerase Homo sapiens 0-3 8178966-1 1994 Intracellular pH (pHi) measurements in frog retinal pigment epithelium using the pH-sensitive dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein demonstrate that the basolateral membrane contains a pHi-sensitive Cl-HCO3 exchanger. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 98-145 glucose-6-phosphate isomerase Homo sapiens 18-21 8178966-1 1994 Intracellular pH (pHi) measurements in frog retinal pigment epithelium using the pH-sensitive dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein demonstrate that the basolateral membrane contains a pHi-sensitive Cl-HCO3 exchanger. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 98-145 glucose-6-phosphate isomerase Homo sapiens 199-202 8178966-1 1994 Intracellular pH (pHi) measurements in frog retinal pigment epithelium using the pH-sensitive dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein demonstrate that the basolateral membrane contains a pHi-sensitive Cl-HCO3 exchanger. Bicarbonates 216-220 glucose-6-phosphate isomerase Homo sapiens 18-21 8019864-4 1994 It was found that glycine residues of proteins have five major clusters in the phi-psi conformational space, and two or three major clusters were found for the other kinds of residues. Glycine 18-25 glucose-6-phosphate isomerase Homo sapiens 79-82 8019864-5 1994 Mode seeking on the potential surface of glycine residues identified their representative phi-psi conformational patterns: (82.64 degrees, 9.84 degrees), (-62.63 degrees, -41.71 degrees), (-85.33 degrees, 176.78 degrees), (91.88 degrees, 178.14 degrees) and (167.06 degrees, -175.33 degrees). Glycine 41-48 glucose-6-phosphate isomerase Homo sapiens 90-93 7510679-1 1994 The urokinase receptor (uPAR) is linked to plasma membranes through a glycosylphosphatidylinositol (GPI) anchor. Glycosylphosphatidylinositols 70-98 glucose-6-phosphate isomerase Homo sapiens 100-103 8160849-7 1994 pHi was also determined using 31P-NMR and found to be the same as that calculated from carnosine alpha-imidazole values. ET bromodomain inhibitor 30-33 glucose-6-phosphate isomerase Homo sapiens 0-3 8160849-7 1994 pHi was also determined using 31P-NMR and found to be the same as that calculated from carnosine alpha-imidazole values. carnosine alpha-imidazole 87-112 glucose-6-phosphate isomerase Homo sapiens 0-3 7511337-7 1994 Ethylisopropylamiloride-sensitive pHi recovery increased from 0.046 +/- 0.007 to 0.076 +/- 0.012 dpHi/min (P < 0.0001). ethylisopropylamiloride 0-23 glucose-6-phosphate isomerase Homo sapiens 34-37 8053092-2 1994 The importance of phosphohexose (PHI) activity in the urine of patients with carcinoma of the urinary bladder is examined on the basis of enzyme-histochemical studies in tumor cell homogenizates after transurethral resection for transitional cell carcinoma. phosphohexose 18-31 glucose-6-phosphate isomerase Homo sapiens 33-36 8007573-4 1994 The thrombin-induced rise in pHi was Na+ dependent and amiloride sensitive, indicating that it was mediated by the Na+/H+ exchanger. Amiloride 55-64 glucose-6-phosphate isomerase Homo sapiens 29-32 8007573-6 1994 In the presence of 1 mmol/liter external calcium (Ca2+o), thrombin-induced increment in [Ca2+]i was significantly greater in patients with EH than in NT (281 +/- 21 vs. 206 +/- 19; P < 0.05) as was the pHi increment (EH: 0.137 +/- 0.01; NT: 0.095 +/- 0.01; P < 0.05). Calcium 41-48 glucose-6-phosphate isomerase Homo sapiens 205-208 8007573-6 1994 In the presence of 1 mmol/liter external calcium (Ca2+o), thrombin-induced increment in [Ca2+]i was significantly greater in patients with EH than in NT (281 +/- 21 vs. 206 +/- 19; P < 0.05) as was the pHi increment (EH: 0.137 +/- 0.01; NT: 0.095 +/- 0.01; P < 0.05). ca2+o 50-55 glucose-6-phosphate isomerase Homo sapiens 205-208 8160784-0 1994 pHi determines rate of sodium transport in frog skin: results of a new method to determine pHi. Sodium 23-29 glucose-6-phosphate isomerase Homo sapiens 0-3 8160784-0 1994 pHi determines rate of sodium transport in frog skin: results of a new method to determine pHi. Sodium 23-29 glucose-6-phosphate isomerase Homo sapiens 91-94 8141263-1 1994 Na(+)-H+ exchange and Na(+)-dependent HCO3- influx both contribute to recovery of intracellular pH (pHi) after an acidosis induced by using the NH4Cl prepulse technique in mammalian and avian cardiac tissue. Bicarbonates 38-42 glucose-6-phosphate isomerase Homo sapiens 100-103 8141263-1 1994 Na(+)-H+ exchange and Na(+)-dependent HCO3- influx both contribute to recovery of intracellular pH (pHi) after an acidosis induced by using the NH4Cl prepulse technique in mammalian and avian cardiac tissue. Ammonium Chloride 144-149 glucose-6-phosphate isomerase Homo sapiens 100-103 8141263-3 1994 pHi was measured from the chemical shift of the exogenous 31P nuclear magnetic resonance pH indicator 2-deoxy-D-glucose 6-phosphate. 2-deoxyglucose-6-phosphate 102-131 glucose-6-phosphate isomerase Homo sapiens 0-3 8141425-1 1994 The true distribution of the pH in tissues can be determined from the in vivo 31P-nuclear magnetic resonance (NMR) spectrum by converting the parts per million (PPM) axis of the pH responsive resonance to pH using the Henderson-Hasselbalch equation. ET bromodomain inhibitor 78-81 glucose-6-phosphate isomerase Homo sapiens 29-31 8141425-1 1994 The true distribution of the pH in tissues can be determined from the in vivo 31P-nuclear magnetic resonance (NMR) spectrum by converting the parts per million (PPM) axis of the pH responsive resonance to pH using the Henderson-Hasselbalch equation. ET bromodomain inhibitor 78-81 glucose-6-phosphate isomerase Homo sapiens 178-180 8141425-1 1994 The true distribution of the pH in tissues can be determined from the in vivo 31P-nuclear magnetic resonance (NMR) spectrum by converting the parts per million (PPM) axis of the pH responsive resonance to pH using the Henderson-Hasselbalch equation. ET bromodomain inhibitor 78-81 glucose-6-phosphate isomerase Homo sapiens 178-180 7514286-3 1994 In the presence of 100 mumol/l Ba2+ the corresponding values were: -70 +/- 1 mV (n = 32), pHi 7.16 +/- 0.08 (n = 6), 0.31 +/- 0.05 (n = 4), 0.06 +/- 0.01 (n = 6), 0.20 +/- 0.03 (n = 10) and -16 mV/pH-unit (n = 15/n = 6). N-methyl-valyl-amiclenomycin 31-35 glucose-6-phosphate isomerase Homo sapiens 90-93 8081251-1 1994 Glycosylphosphatidylinositol(GPI) anchor-hydrolyzing phospholipases include C- and D-type phospholipases and have been described in a number of organisms including bacteria, protozoan parasites, plants, and mammals. Glycosylphosphatidylinositols 0-28 glucose-6-phosphate isomerase Homo sapiens 29-32 7514286-4 1994 In the presence of 500 mumol/l amiloride the corresponding values were: -72 +/- 2 mV (n = 34), pHi 7.00 +/- 0.07 (n = 5), 0.50 +/- 0.04 (n = 6), 0.04 +/- 0.01 (n = 11), 0.28 +/- 0.04 (n = 9) and -26 mV/pH-unit (n = 20/n = 5). Amiloride 31-40 glucose-6-phosphate isomerase Homo sapiens 95-98 7514286-5 1994 In the presence of 20 mmol/l propionate plus amiloride the corresponding values were: -61 +/- 2 mV (n = 27), pHi 6.72 +/- 0.06 (n = 5), 0.30 +/- 0.02 (n = 6), 0.06 +/- 0.01 (n = 5) and 0.40 +/- 0.02 (n = 8), respectively. Propionates 29-39 glucose-6-phosphate isomerase Homo sapiens 109-112 7514286-5 1994 In the presence of 20 mmol/l propionate plus amiloride the corresponding values were: -61 +/- 2 mV (n = 27), pHi 6.72 +/- 0.06 (n = 5), 0.30 +/- 0.02 (n = 6), 0.06 +/- 0.01 (n = 5) and 0.40 +/- 0.02 (n = 8), respectively. Amiloride 45-54 glucose-6-phosphate isomerase Homo sapiens 109-112 8304409-3 1994 pHi was controlled by a Na(+)-H+ antiporter that was reversible, electroneutral, inhibited by 5-(N-methyl-N-isobutyl)amiloride, and dependent on extracellular Na+. 5-(N-methyl-N-isobutyl)amiloride 94-126 glucose-6-phosphate isomerase Homo sapiens 0-3 8280110-7 1994 The separate chelation of Ca2+i (30 microM BAPTA-AM) or inhibition of PKC (1 microM staurosporine) induced about 50% inhibition of the pHi responses triggered by TRAP, trypsin, gamma- and alpha-thrombin, but the combination induced complete inhibition. ca2+i 26-31 glucose-6-phosphate isomerase Homo sapiens 135-138 8280110-7 1994 The separate chelation of Ca2+i (30 microM BAPTA-AM) or inhibition of PKC (1 microM staurosporine) induced about 50% inhibition of the pHi responses triggered by TRAP, trypsin, gamma- and alpha-thrombin, but the combination induced complete inhibition. 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester 43-51 glucose-6-phosphate isomerase Homo sapiens 135-138 8280110-7 1994 The separate chelation of Ca2+i (30 microM BAPTA-AM) or inhibition of PKC (1 microM staurosporine) induced about 50% inhibition of the pHi responses triggered by TRAP, trypsin, gamma- and alpha-thrombin, but the combination induced complete inhibition. Staurosporine 84-97 glucose-6-phosphate isomerase Homo sapiens 135-138 7982029-10 1994 Authors suggest that weak cell reactivity may be connected with alterations in cell membranes, mainly low phosphatidylinositol (GPI) content. Phosphatidylinositols 106-126 glucose-6-phosphate isomerase Homo sapiens 128-131 8304433-5 1994 Steady-state ATP-dependent pHi in the three vesicles was more similar. Adenosine Triphosphate 13-16 glucose-6-phosphate isomerase Homo sapiens 27-30 8269987-2 1994 Specifically, a glycosylphosphatidylinositol (GPI)-modified variant of the cytokine macrophage colony stimulating factor (M-CSF), designated M-CSF.GPI, was expressed on the surface of human bone marrow stromal cells. Glycosylphosphatidylinositols 16-44 glucose-6-phosphate isomerase Homo sapiens 46-49 8201088-9 1994 Of the patients (n = 7) with low pHi (7.2 +/- 0.13) and a pHL > 4 (6.51 +/- 0.48) only one responded to pentagastrin (decrease in pHL for this group 0.93 +/- 1.86). Pentagastrin 107-119 glucose-6-phosphate isomerase Homo sapiens 33-36 8258824-9 1993 Ionophores such as nigericin, CCCP, or propionic acid also lowered the pHi but did not inhibit the oxidative burst, indicating that the pHi drop was not the cause for this inhibition. Nigericin 19-28 glucose-6-phosphate isomerase Homo sapiens 71-74 8265579-8 1993 However, virtually complete and reversible block of the response was achieved when cytoplasmic pH (pHi) was brought under experimental control using the weak acid butyrate. Butyrates 163-171 glucose-6-phosphate isomerase Homo sapiens 99-102 8265579-9 1993 Parallel measurements of pHi using the fluorescent dye 2",7"-bis(2-carboxyethyl-5(6)-carboxyfluorescein (BCECF) and dual-wavelength laser-scanning confocal microscopy demonstrated that the C-terminal peptide evoked rapid and reversible cytoplasmic alkalinizations of 0.4 +/- 0.1 pHi unit and confirmed the antagonism of the pHi response in the presence of butyrate. 2",7"-bis(2-carboxyethyl-5(6)-carboxyfluorescein 55-103 glucose-6-phosphate isomerase Homo sapiens 25-28 8265579-10 1993 These, and comparable results with the auxins indole acetic acid and 1-naphthyleneacetic acid, implicate the C-terminal domain of ABPzm1 in auxin-ABP coupling to pHi and an associated intracellular signaling cascade. 1-naphthyleneacetic acid 69-93 glucose-6-phosphate isomerase Homo sapiens 162-165 8258824-9 1993 Ionophores such as nigericin, CCCP, or propionic acid also lowered the pHi but did not inhibit the oxidative burst, indicating that the pHi drop was not the cause for this inhibition. Carbonyl Cyanide m-Chlorophenyl Hydrazone 30-34 glucose-6-phosphate isomerase Homo sapiens 71-74 8258824-9 1993 Ionophores such as nigericin, CCCP, or propionic acid also lowered the pHi but did not inhibit the oxidative burst, indicating that the pHi drop was not the cause for this inhibition. propionic acid 39-53 glucose-6-phosphate isomerase Homo sapiens 71-74 7918046-2 1993 aCL was positive in all 16 SLE patients and all 15 syphilis patients, who were positive for aCL in the standard ELISA, in the GPI-independent ELISA with Tween 20. Polysorbates 153-161 glucose-6-phosphate isomerase Homo sapiens 126-129 8244517-10 1993 Endothelin-1 increased pHi through endothelin A-receptors (effect blocked by the specific antagonist BQ-123) but had no significant effect on [Ca2+]i or aggregation. cyclo(Trp-Asp-Pro-Val-Leu) 101-107 glucose-6-phosphate isomerase Homo sapiens 23-26 7918046-3 1993 GPI-dependent aCL was detected in 12/16 SLE patients by the GPI-dependent ELISA with Tween 20. Polysorbates 85-93 glucose-6-phosphate isomerase Homo sapiens 0-3 7918046-3 1993 GPI-dependent aCL was detected in 12/16 SLE patients by the GPI-dependent ELISA with Tween 20. Polysorbates 85-93 glucose-6-phosphate isomerase Homo sapiens 60-63 7918046-5 1993 On the basis of these results, we recommend that Tween 20 should be used in ELISAs to distinguish GPI-dependent aCL from GPI-independent aCL. Polysorbates 49-57 glucose-6-phosphate isomerase Homo sapiens 98-101 7918046-5 1993 On the basis of these results, we recommend that Tween 20 should be used in ELISAs to distinguish GPI-dependent aCL from GPI-independent aCL. Polysorbates 49-57 glucose-6-phosphate isomerase Homo sapiens 121-124 8273285-5 1993 Amiloride (2 mM) also caused a decrease of photoresponse amplitude, which suggests that Na+/H+ exchange contributes to pHi regulation. Amiloride 0-9 glucose-6-phosphate isomerase Homo sapiens 119-122 8224234-0 1993 Effects of repetitive stimulation, veratridine and ouabain on cytoplasmic pH in frog nerve fibres: role of internal Na+. Ouabain 51-58 glucose-6-phosphate isomerase Homo sapiens 74-76 8224234-1 1993 Changes of cytoplasmic pH (pHi) in frog nerve fibres during repetitive stimulation have been measured using the fluorescent pH indicator dye fluorescein diacetate (FDA). diacetylfluorescein 141-162 glucose-6-phosphate isomerase Homo sapiens 23-25 8224234-1 1993 Changes of cytoplasmic pH (pHi) in frog nerve fibres during repetitive stimulation have been measured using the fluorescent pH indicator dye fluorescein diacetate (FDA). diacetylfluorescein 141-162 glucose-6-phosphate isomerase Homo sapiens 27-29 8224234-1 1993 Changes of cytoplasmic pH (pHi) in frog nerve fibres during repetitive stimulation have been measured using the fluorescent pH indicator dye fluorescein diacetate (FDA). diacetylfluorescein 164-167 glucose-6-phosphate isomerase Homo sapiens 23-25 8224234-1 1993 Changes of cytoplasmic pH (pHi) in frog nerve fibres during repetitive stimulation have been measured using the fluorescent pH indicator dye fluorescein diacetate (FDA). diacetylfluorescein 164-167 glucose-6-phosphate isomerase Homo sapiens 27-29 8309782-1 1993 The intracellular pH (pHi) of the colonic tumour cell line HT29 cl.19A was studied by microspectrofluorometry using the pH-sensitive dye BCECF. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 137-142 glucose-6-phosphate isomerase Homo sapiens 22-25 7901350-0 1993 Changes in intracellular pH associated with glutamate excitotoxicity. Glutamic Acid 44-53 glucose-6-phosphate isomerase Homo sapiens 25-27 7901350-5 1993 During the hour after GLU removal, intracellular pH (pHi) recovered steadily, resulting in a rebound cytosolic alkalinization. Glutamic Acid 22-25 glucose-6-phosphate isomerase Homo sapiens 49-51 7901350-5 1993 During the hour after GLU removal, intracellular pH (pHi) recovered steadily, resulting in a rebound cytosolic alkalinization. Glutamic Acid 22-25 glucose-6-phosphate isomerase Homo sapiens 53-56 8166793-7 1993 The predictor variable, pHi, was calculated using the following equation: 6.1 + log HCO3/(gastric PCO2 x 0.0307). Bicarbonates 84-88 glucose-6-phosphate isomerase Homo sapiens 24-27 8303648-6 1993 In contrast, the OD readings of 6 control patients with systemic lupus erythematosus (SLE) increased markedly (3/6) or decreased slightly to moderately (3/6), however, within the limits of positive range, indicating GPI-dependent cCL positivity. Cefaclor 230-233 glucose-6-phosphate isomerase Homo sapiens 216-219 8238296-1 1993 Mammalian cells generally regulate their intracellular pH (pHi) via collaboration between Na(+)-H+ exchanger and HCO3- transport. Bicarbonates 113-117 glucose-6-phosphate isomerase Homo sapiens 59-62 8238296-5 1993 Bafilomycin A1 decreased pHi in the six tumor cell lines with the highest resting pHi in the absence of HCO3-. bafilomycin 0-11 glucose-6-phosphate isomerase Homo sapiens 25-28 7693667-5 1993 Because IBMX antagonizes this action of NH4Cl, these results further suggest that elevation of pHi initiates an inactivation of guanylyl cyclase that leads to K channel closure. Ammonium Chloride 40-45 glucose-6-phosphate isomerase Homo sapiens 95-98 7693668-0 1993 Activation of Ca2+ permeability by cAMP is coordinated through the pHi increase induced by speract. Cyclic AMP 35-39 glucose-6-phosphate isomerase Homo sapiens 67-70 7693668-9 1993 This study provides tentative identification of sperm Ca channels as downstream targets of cAMP action and indicates that pHi may determine whether cGMP- or cAMP-mediated second messenger pathways predominate in speract signal transduction. Cyclic GMP 148-152 glucose-6-phosphate isomerase Homo sapiens 122-125 7693668-9 1993 This study provides tentative identification of sperm Ca channels as downstream targets of cAMP action and indicates that pHi may determine whether cGMP- or cAMP-mediated second messenger pathways predominate in speract signal transduction. Cyclic AMP 157-161 glucose-6-phosphate isomerase Homo sapiens 122-125 8238296-5 1993 Bafilomycin A1 decreased pHi in the six tumor cell lines with the highest resting pHi in the absence of HCO3-. bafilomycin 0-11 glucose-6-phosphate isomerase Homo sapiens 82-85 8166793-7 1993 The predictor variable, pHi, was calculated using the following equation: 6.1 + log HCO3/(gastric PCO2 x 0.0307). pco2 98-102 glucose-6-phosphate isomerase Homo sapiens 24-27 8117250-3 1993 At the onset of ischemia intracellular pH (pHi) decreases due to anaerobic metabolism and ATP hydrolysis, leading to an activation of Na+/H+ exchange. Adenosine Triphosphate 90-93 glucose-6-phosphate isomerase Homo sapiens 43-46 8270911-8 1993 In suspended cells, recovery of the cytosolic pH (pHi) from an acid-load in Na+ and HCO3(-)-free medium was detectable in depolarizing but not in hyperpolarizing media. Bicarbonates 84-89 glucose-6-phosphate isomerase Homo sapiens 50-53 8274711-1 1993 Dipeptide carnosine effect on fibroblast proliferation was assessed by changes in intracellular pH (pHi) in human cultivated lung embryonal fibroblasts. dipeptide carnosine 0-19 glucose-6-phosphate isomerase Homo sapiens 96-98 8214077-4 1993 Exposure to 130 mM propionate in isosmotic medium causes a rapid decrease in pHi and activates pHi recovery via amiloride-sensitive Na-H exchange. Propionates 19-29 glucose-6-phosphate isomerase Homo sapiens 77-80 8214077-4 1993 Exposure to 130 mM propionate in isosmotic medium causes a rapid decrease in pHi and activates pHi recovery via amiloride-sensitive Na-H exchange. Propionates 19-29 glucose-6-phosphate isomerase Homo sapiens 95-98 8214077-4 1993 Exposure to 130 mM propionate in isosmotic medium causes a rapid decrease in pHi and activates pHi recovery via amiloride-sensitive Na-H exchange. Amiloride 112-121 glucose-6-phosphate isomerase Homo sapiens 95-98 8245824-3 1993 A stable decrease in the pHi of 0.52 +/- 0.05 U (n = 16) was obtained by switching from a bicarbonate buffer equilibrated with 5% CO2 to a buffer equilibrated with 20% CO2. Bicarbonates 90-101 glucose-6-phosphate isomerase Homo sapiens 25-28 8245824-3 1993 A stable decrease in the pHi of 0.52 +/- 0.05 U (n = 16) was obtained by switching from a bicarbonate buffer equilibrated with 5% CO2 to a buffer equilibrated with 20% CO2. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 130-133 glucose-6-phosphate isomerase Homo sapiens 25-28 8245824-3 1993 A stable decrease in the pHi of 0.52 +/- 0.05 U (n = 16) was obtained by switching from a bicarbonate buffer equilibrated with 5% CO2 to a buffer equilibrated with 20% CO2. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 168-171 glucose-6-phosphate isomerase Homo sapiens 25-28 8226330-1 1993 The cytosolic pH (pHi) of transitional cells from the ampulla of the gerbil was measured micro-fluorometrically with the pH-sensitive dye 2",7"-bicarboxyethyl-5(6)-carboxyfluorescein (BCECF) to assess the possible contribution of a Na+/H+ exchanger to the regulation of pHi. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 184-189 glucose-6-phosphate isomerase Homo sapiens 18-21 8226330-3 1993 Under control conditions, pHi was 7.19 and addition of 10(-5) M ethylisopropylamiloride (EIPA), a blocker of Na+/H+ exchange, caused a small but significant acidification of pHi. ethylisopropylamiloride 64-87 glucose-6-phosphate isomerase Homo sapiens 174-177 8226330-3 1993 Under control conditions, pHi was 7.19 and addition of 10(-5) M ethylisopropylamiloride (EIPA), a blocker of Na+/H+ exchange, caused a small but significant acidification of pHi. ethylisopropylamiloride 89-93 glucose-6-phosphate isomerase Homo sapiens 174-177 8226330-4 1993 A transient exposure to 21.4 mM NH4Cl caused a rapid cytosolic alkalinization followed by a brisk acidification and prompt recovery of pHi to its control value. Ammonium Chloride 32-37 glucose-6-phosphate isomerase Homo sapiens 135-138 8226330-6 1993 Bi was 4.7 mM/pH at pHi 7.19 and varied with pHi. Bismuth 0-2 glucose-6-phosphate isomerase Homo sapiens 20-23 8226330-6 1993 Bi was 4.7 mM/pH at pHi 7.19 and varied with pHi. Bismuth 0-2 glucose-6-phosphate isomerase Homo sapiens 45-48 8274711-1 1993 Dipeptide carnosine effect on fibroblast proliferation was assessed by changes in intracellular pH (pHi) in human cultivated lung embryonal fibroblasts. dipeptide carnosine 0-19 glucose-6-phosphate isomerase Homo sapiens 100-103 8274711-2 1993 pHi was defined by means of fluorescein diacetate (FDA) during 1-7 days of the culture growth. diacetylfluorescein 28-49 glucose-6-phosphate isomerase Homo sapiens 0-3 8274711-2 1993 pHi was defined by means of fluorescein diacetate (FDA) during 1-7 days of the culture growth. diacetylfluorescein 51-54 glucose-6-phosphate isomerase Homo sapiens 0-3 8274711-3 1993 The investigation showed changes in pHi depending on dipeptide concentration and the presence of additives in it. Dipeptides 53-62 glucose-6-phosphate isomerase Homo sapiens 36-39 8274711-6 1993 In this case dipeptide was assumed to have a stimulating influence on fibroblast pHi due to a low concentration of additives in the drug and fast adaptation of cells. Dipeptides 13-22 glucose-6-phosphate isomerase Homo sapiens 81-84 8392591-2 1993 To identify the precise location of the cellular kinase-mediated phosphorylation, we generated a tailless deletion mutant and several point mutants which had altered serine and threonine residues within the cytoplasmic domain of gpI. Threonine 177-186 glucose-6-phosphate isomerase Homo sapiens 229-232 8392591-5 1993 Examination of the point mutants established that the major phosphorylation sequence of gpI was located between amino acids 593 and 598, a site which included four phosphorylatable serine and threonine residues. Serine 181-187 glucose-6-phosphate isomerase Homo sapiens 88-91 8392591-5 1993 Examination of the point mutants established that the major phosphorylation sequence of gpI was located between amino acids 593 and 598, a site which included four phosphorylatable serine and threonine residues. Threonine 192-201 glucose-6-phosphate isomerase Homo sapiens 88-91 8392591-6 1993 Phosphorylation analyses of the mutant and wild-type glycoproteins confirmed that gpI was a substrate for casein kinase II, with threonines 596 and 598 being critical residues. Threonine 129-139 glucose-6-phosphate isomerase Homo sapiens 82-85 8392591-8 1993 Thus, these mutagenesis studies demonstrated that the gpI cytoplasmic sequence Ser-Glu-Ser-Thr-Asp-Thr was phosphorylated in mammalian cells in the absence of any other herpesvirus products. Serine 79-82 glucose-6-phosphate isomerase Homo sapiens 54-57 8392591-8 1993 Thus, these mutagenesis studies demonstrated that the gpI cytoplasmic sequence Ser-Glu-Ser-Thr-Asp-Thr was phosphorylated in mammalian cells in the absence of any other herpesvirus products. Glutamic Acid 83-86 glucose-6-phosphate isomerase Homo sapiens 54-57 8392591-8 1993 Thus, these mutagenesis studies demonstrated that the gpI cytoplasmic sequence Ser-Glu-Ser-Thr-Asp-Thr was phosphorylated in mammalian cells in the absence of any other herpesvirus products. ser-thr-asp-thr 87-102 glucose-6-phosphate isomerase Homo sapiens 54-57 7692041-2 1993 To determine whether conductive pathways contribute to the H+ efflux from granulocytes, we used the whole-cell patch-clamp technique combined with microfluorimetric determinations of cytosolic pH (pHi) in single, dimethylsulphoxide-differentiated HL-60 cells. Dimethyl Sulfoxide 213-231 glucose-6-phosphate isomerase Homo sapiens 197-200 8393626-3 1993 pHi was measured from the phosphorus-31 nuclear magnetic resonance chemical shift of 2-deoxy-D-glucose 6-phosphate. Phosphorus 26-36 glucose-6-phosphate isomerase Homo sapiens 0-3 8393626-3 1993 pHi was measured from the phosphorus-31 nuclear magnetic resonance chemical shift of 2-deoxy-D-glucose 6-phosphate. 2-deoxyglucose-6-phosphate 85-114 glucose-6-phosphate isomerase Homo sapiens 0-3 8393626-4 1993 Basal pHi was higher in HCO(3-)-buffered solution (7.05 +/- 0.01; n = 8) than in nominally HCO(3-)-free N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES) solution (6.98 +/- 0.02; n = 9). Bicarbonates 24-31 glucose-6-phosphate isomerase Homo sapiens 6-9 8393626-4 1993 Basal pHi was higher in HCO(3-)-buffered solution (7.05 +/- 0.01; n = 8) than in nominally HCO(3-)-free N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES) solution (6.98 +/- 0.02; n = 9). Bicarbonates 91-98 glucose-6-phosphate isomerase Homo sapiens 6-9 8393626-4 1993 Basal pHi was higher in HCO(3-)-buffered solution (7.05 +/- 0.01; n = 8) than in nominally HCO(3-)-free N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES) solution (6.98 +/- 0.02; n = 9). HEPES 157-162 glucose-6-phosphate isomerase Homo sapiens 6-9 8393626-5 1993 Addition of 5-(N-ethyl-N-isopropyl)amiloride (EIPA) caused a significant fall in pHi in HEPES solution (6.91 +/- 0.02; n = 5) but not in HCO3- solution (7.02 +/- 0.02; n = 5). ethylisopropylamiloride 12-44 glucose-6-phosphate isomerase Homo sapiens 81-84 8393626-5 1993 Addition of 5-(N-ethyl-N-isopropyl)amiloride (EIPA) caused a significant fall in pHi in HEPES solution (6.91 +/- 0.02; n = 5) but not in HCO3- solution (7.02 +/- 0.02; n = 5). ethylisopropylamiloride 46-50 glucose-6-phosphate isomerase Homo sapiens 81-84 8393626-5 1993 Addition of 5-(N-ethyl-N-isopropyl)amiloride (EIPA) caused a significant fall in pHi in HEPES solution (6.91 +/- 0.02; n = 5) but not in HCO3- solution (7.02 +/- 0.02; n = 5). HEPES 88-93 glucose-6-phosphate isomerase Homo sapiens 81-84 8393626-7 1993 After an intracellular acidosis induced by an NH4Cl prepulse, the proton efflux rate (JH) at pHi 6.90 was 0.5 +/- 0.2 nmol.l-1.min-1 (n = 14) in HEPES solution and 1.2 +/- 0.4 mmol.l-1.min-1 (n = 13) in HCO3- solution. Ammonium Chloride 46-51 glucose-6-phosphate isomerase Homo sapiens 93-96 8393626-7 1993 After an intracellular acidosis induced by an NH4Cl prepulse, the proton efflux rate (JH) at pHi 6.90 was 0.5 +/- 0.2 nmol.l-1.min-1 (n = 14) in HEPES solution and 1.2 +/- 0.4 mmol.l-1.min-1 (n = 13) in HCO3- solution. HEPES 145-150 glucose-6-phosphate isomerase Homo sapiens 93-96 8393626-7 1993 After an intracellular acidosis induced by an NH4Cl prepulse, the proton efflux rate (JH) at pHi 6.90 was 0.5 +/- 0.2 nmol.l-1.min-1 (n = 14) in HEPES solution and 1.2 +/- 0.4 mmol.l-1.min-1 (n = 13) in HCO3- solution. Bicarbonates 203-207 glucose-6-phosphate isomerase Homo sapiens 93-96 8393626-8 1993 The addition of 1 microM EIPA effectively blocked proton efflux in HEPES solution (JH < 0.1 mmol.l-1.min-1; n = 8), whereas it slowed pHi recovery in HCO3- solution (JH = 0.6 +/- 0.2 mmol.l-1.min-1; n = 9). ethylisopropylamiloride 25-29 glucose-6-phosphate isomerase Homo sapiens 137-140 8393626-10 1993 The Na(+)-H+ antiport and a mechanism requiring both external Na+ and HCO3- each contribute approximately 50% to proton efflux at pHi 6.90 during the recovery from intracellular acidosis in the isolated perfused mammalian heart. sodium bisulfide 4-12 glucose-6-phosphate isomerase Homo sapiens 130-133 8393626-10 1993 The Na(+)-H+ antiport and a mechanism requiring both external Na+ and HCO3- each contribute approximately 50% to proton efflux at pHi 6.90 during the recovery from intracellular acidosis in the isolated perfused mammalian heart. Bicarbonates 70-74 glucose-6-phosphate isomerase Homo sapiens 130-133 8338158-1 1993 Hepatocytes possess several mechanisms for membrane acid-base transport, which work in concert to maintain intracellular pH (pHi) in a narrow physiological range, despite metabolic processes that produce and consume substantial quantities of H+ and HCO3-.Na(+)-H+ and Cl(-)-HCO3- exchangers contribute to recovery from intracellular acidosis and alkalosis, respectively, but are largely inoperative at physiological values of pHi. sodium bisulfide 255-263 glucose-6-phosphate isomerase Homo sapiens 125-128 8338158-4 1993 In this review, the properties of hepatic Na(+)-HCO3- cotransport are described with emphasis on its effects on pHi and Na+ homeostasis and on the possible role of membrane potential difference as a signal modulating the rate of HCO3- influx and pHi of hepatocytes through effects on this transporter. Bicarbonates 48-52 glucose-6-phosphate isomerase Homo sapiens 112-115 8338158-4 1993 In this review, the properties of hepatic Na(+)-HCO3- cotransport are described with emphasis on its effects on pHi and Na+ homeostasis and on the possible role of membrane potential difference as a signal modulating the rate of HCO3- influx and pHi of hepatocytes through effects on this transporter. Bicarbonates 48-52 glucose-6-phosphate isomerase Homo sapiens 246-249 8389195-1 1993 In the present study, we investigated whether stimulation of platelets by epinephrine affects the Na+/H+ exchanger, an antiport that regulates the cytosolic pH (pHi). Epinephrine 74-85 glucose-6-phosphate isomerase Homo sapiens 161-164 8476022-5 1993 This Cai2+ increase was most apparent when pHi was very low (< 6.6) and was unaffected by removal of external Ca2+ or NiCl2 addition. cai2+ 5-10 glucose-6-phosphate isomerase Homo sapiens 43-46 8388419-4 1993 When Na+/H+ exchange was blocked with 10 microM DMA, the pHi of NKC bound to NK-sensitive K562 target cells progressively decreased for 3 to 4 min, then stabilized at 0.1 to 0.15 pH units below the pHi of NKU. 5-dimethylamiloride 48-51 glucose-6-phosphate isomerase Homo sapiens 57-60 8388419-4 1993 When Na+/H+ exchange was blocked with 10 microM DMA, the pHi of NKC bound to NK-sensitive K562 target cells progressively decreased for 3 to 4 min, then stabilized at 0.1 to 0.15 pH units below the pHi of NKU. 5-dimethylamiloride 48-51 glucose-6-phosphate isomerase Homo sapiens 198-201 8388419-8 1993 Manipulations of HCO3- and DMA that clamped NKC pHi at values ranging from 6.8 to 7.2 failed to significantly influence NK cell cytolytic function. Bicarbonates 17-21 glucose-6-phosphate isomerase Homo sapiens 48-51 8388419-8 1993 Manipulations of HCO3- and DMA that clamped NKC pHi at values ranging from 6.8 to 7.2 failed to significantly influence NK cell cytolytic function. 5-dimethylamiloride 27-30 glucose-6-phosphate isomerase Homo sapiens 48-51 8496158-2 1993 In this paper, the effect of ManN on the synthesis of putative GPI anchor precursors in mammalian cells was studied. mannosamine 29-33 glucose-6-phosphate isomerase Homo sapiens 63-66 8388633-3 1993 In addition, basal pHi (measured in the absence of bicarbonate) increased from 7.15 to 7.26, suggesting an alteration in the antiporter"s "set point" during HL-60 cell differentiation. Bicarbonates 51-62 glucose-6-phosphate isomerase Homo sapiens 19-22 8512665-5 1993 At each point in time (from t = 0 to t = 60, 120, 180 min), glucose induced significant (P < .05) elevations in Cai (27.2 +/- 2.2 to 68.3 +/- 7.2, 70.7 +/- 10.5, 59.8 +/- 10.1 nmol/L), while suppressing pHi (7.28 +/- 0.02 to 7.22 +/- 0.03, 7.23 +/- 0.03, 7.22 +/- 0.03), and Mgi (206 +/- 10 to 151 +/- 7, 131 +/- 7, 143 +/- 5 mumol/L). Glucose 60-67 glucose-6-phosphate isomerase Homo sapiens 206-209 8315350-9 1993 The chemotactic factor-induced cytoplasmic pH responses of monocytes/macrophages remained constant as the cells matured in vitro and exhibited a dimethylamiloride-independent acidification and dependent alkalinization, as did the response in neutrophils. 5-dimethylamiloride 145-162 glucose-6-phosphate isomerase Homo sapiens 43-45 8210461-1 1993 It has previously been reported that amiloride, a diuretic drug, sensitizes cells to hyperthermia by inhibiting the Na+/H+ exchange through the plasma membrane and thus decreasing the intracellular pH (pHi), particularly in a low extracellular pH (pHe) environment. Amiloride 37-46 glucose-6-phosphate isomerase Homo sapiens 202-205 8210461-1 1993 It has previously been reported that amiloride, a diuretic drug, sensitizes cells to hyperthermia by inhibiting the Na+/H+ exchange through the plasma membrane and thus decreasing the intracellular pH (pHi), particularly in a low extracellular pH (pHe) environment. Phenylalanine 248-251 glucose-6-phosphate isomerase Homo sapiens 202-205 8210461-2 1993 In the present study, the efficacy of 5-(N-ethyl-N-isopropyl) amiloride (EIPA), an analog of amiloride, to lower the pHi and sensitize tumor cells to hyperthermia was investigated. ethylisopropylamiloride 38-71 glucose-6-phosphate isomerase Homo sapiens 117-120 8210461-2 1993 In the present study, the efficacy of 5-(N-ethyl-N-isopropyl) amiloride (EIPA), an analog of amiloride, to lower the pHi and sensitize tumor cells to hyperthermia was investigated. ethylisopropylamiloride 73-77 glucose-6-phosphate isomerase Homo sapiens 117-120 8210461-2 1993 In the present study, the efficacy of 5-(N-ethyl-N-isopropyl) amiloride (EIPA), an analog of amiloride, to lower the pHi and sensitize tumor cells to hyperthermia was investigated. Amiloride 62-71 glucose-6-phosphate isomerase Homo sapiens 117-120 8210461-3 1993 It was observed that 10 microM EIPA was as effective as 500 microM amiloride to lower the pHi and to increase the thermal sensitivity of SCK tumor cells in vitro. ethylisopropylamiloride 31-35 glucose-6-phosphate isomerase Homo sapiens 90-93 8210461-3 1993 It was observed that 10 microM EIPA was as effective as 500 microM amiloride to lower the pHi and to increase the thermal sensitivity of SCK tumor cells in vitro. Amiloride 67-76 glucose-6-phosphate isomerase Homo sapiens 90-93 8498523-0 1993 pH-induced calcium transients in type II alveolar epithelial cells. Calcium 11-18 glucose-6-phosphate isomerase Homo sapiens 0-2 8498523-5 1993 pHi increased rapidly after addition of 25 mM NH4Cl in both cultured and freshly isolated cells and then decreased back toward baseline over the following 10 min. Ammonium Chloride 46-51 glucose-6-phosphate isomerase Homo sapiens 0-3 8498523-6 1993 The rise in pHi was associated with a transient increase in [Ca2+]i. Resuspension of cells in an NH4Cl-free solution resulted in rapid intracellular acidification, which recovered over the subsequent 10 min. Ammonium Chloride 97-102 glucose-6-phosphate isomerase Homo sapiens 12-15 8507440-6 1993 Furthermore, despite the predominant use of red cells as a tissue source, the measured levels of Cai, Mgi, and pHi were closely related to the ambient blood pressure, the degree of cardiac hypertrophy, and to the hyperinsulinemic response to oral glucose challenge. Glucose 247-254 glucose-6-phosphate isomerase Homo sapiens 111-114 8383969-1 1993 The aim of the present study was to evaluate the regulatory relationship between the cytosolic free calcium concentration ([Ca2+]i and cytosolic pH (pHi). Calcium 100-107 glucose-6-phosphate isomerase Homo sapiens 149-152 8383969-9 1993 Moreover, MAPTAM-loaded calcium-depleted platelets had a basal pHi that was more acidic than in the presence of 1 mmol/l extracellular calcium (6.93 +/- 0.09 versus 7.14 +/- 0.01, n = 26, P < 0.001). Calcium 24-31 glucose-6-phosphate isomerase Homo sapiens 63-66 8383969-2 1993 [Ca2+]i and pHi were measured using the fluorescent dyes fura-2 and BCECF [2",7"-bis-(carboxyethyl)-5,6-carboxyfluorescein] respectively. Fura-2 57-63 glucose-6-phosphate isomerase Homo sapiens 12-15 8383969-10 1993 Ionomycin induced an elevation of [Ca2+]i that was accompanied by a concomitant increase in pHi, which was Na(+)-dependent and amiloride-sensitive. Ionomycin 0-9 glucose-6-phosphate isomerase Homo sapiens 92-95 8383969-10 1993 Ionomycin induced an elevation of [Ca2+]i that was accompanied by a concomitant increase in pHi, which was Na(+)-dependent and amiloride-sensitive. Amiloride 127-136 glucose-6-phosphate isomerase Homo sapiens 92-95 8383969-2 1993 [Ca2+]i and pHi were measured using the fluorescent dyes fura-2 and BCECF [2",7"-bis-(carboxyethyl)-5,6-carboxyfluorescein] respectively. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 68-73 glucose-6-phosphate isomerase Homo sapiens 12-15 8383969-11 1993 [Ca2+]i and pHi increases induced by ionomycin were both dependent on the concentration of ionomycin. Ionomycin 37-46 glucose-6-phosphate isomerase Homo sapiens 12-15 8383969-11 1993 [Ca2+]i and pHi increases induced by ionomycin were both dependent on the concentration of ionomycin. Ionomycin 91-100 glucose-6-phosphate isomerase Homo sapiens 12-15 8383969-8 1993 When platelets were preloaded with the intracellular calcium chelator MAPTAM (1,2-bis-5-methylaminophenoxylethane-NNN"-tetraacetoxymethyl acetate) to block the increase in [Ca2+]i induced by thrombin, no increment in pHi was observed. 5,5'-dimethyl-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate 70-76 glucose-6-phosphate isomerase Homo sapiens 217-220 8383969-9 1993 Moreover, MAPTAM-loaded calcium-depleted platelets had a basal pHi that was more acidic than in the presence of 1 mmol/l extracellular calcium (6.93 +/- 0.09 versus 7.14 +/- 0.01, n = 26, P < 0.001). 5,5'-dimethyl-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate 10-16 glucose-6-phosphate isomerase Homo sapiens 63-66 8331595-2 1993 Neutral-carrier pH-sensitive microelectrodes were used to investigate intracellular pH (pHi) recovery from alkalinization in leech Retzius neurones in Hepes- and in CO2-HCO3(-)-buffered solution. HEPES 151-156 glucose-6-phosphate isomerase Homo sapiens 84-86 8436610-12 1993 In the hyperglycemic groups, depolarization occurred at a lactate content of about 12 mm kg-1, corresponding to a pHi of approximately 6.4. Lactic Acid 58-65 glucose-6-phosphate isomerase Homo sapiens 114-117 8331595-2 1993 Neutral-carrier pH-sensitive microelectrodes were used to investigate intracellular pH (pHi) recovery from alkalinization in leech Retzius neurones in Hepes- and in CO2-HCO3(-)-buffered solution. HEPES 151-156 glucose-6-phosphate isomerase Homo sapiens 88-91 8511671-3 1993 PNH cells are deficient in proteins attached to the cell membrane via a glycosylphosphatidylinositol structure, called the GPI anchor, and the primary lesion in PNH is thought to be a defect in the biosynthesis of the GPI anchor. Glycosylphosphatidylinositols 72-100 glucose-6-phosphate isomerase Homo sapiens 123-126 8331595-18 1993 In Cl(-)-free, CO2-HCO3(-)-buffered solution pHi recovery from an alkaline load by changing from 5% CO2-27 mM HCO3- to 2% CO2-11 mM HCO3- was slowed by 48-71%. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 15-18 glucose-6-phosphate isomerase Homo sapiens 45-48 8331595-18 1993 In Cl(-)-free, CO2-HCO3(-)-buffered solution pHi recovery from an alkaline load by changing from 5% CO2-27 mM HCO3- to 2% CO2-11 mM HCO3- was slowed by 48-71%. Bicarbonates 19-23 glucose-6-phosphate isomerase Homo sapiens 45-48 8331595-5 1993 In Hepes-buffered solution (pH 7.4) the mean pHi was 7.29 +/- 0.11 and the mean membrane potential -44.7 +/- 5.9 mV (mean +/- S.D. HEPES 3-8 glucose-6-phosphate isomerase Homo sapiens 28-30 8331595-18 1993 In Cl(-)-free, CO2-HCO3(-)-buffered solution pHi recovery from an alkaline load by changing from 5% CO2-27 mM HCO3- to 2% CO2-11 mM HCO3- was slowed by 48-71%. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 100-103 glucose-6-phosphate isomerase Homo sapiens 45-48 8331595-5 1993 In Hepes-buffered solution (pH 7.4) the mean pHi was 7.29 +/- 0.11 and the mean membrane potential -44.7 +/- 5.9 mV (mean +/- S.D. HEPES 3-8 glucose-6-phosphate isomerase Homo sapiens 45-48 8331595-18 1993 In Cl(-)-free, CO2-HCO3(-)-buffered solution pHi recovery from an alkaline load by changing from 5% CO2-27 mM HCO3- to 2% CO2-11 mM HCO3- was slowed by 48-71%. Bicarbonates 110-114 glucose-6-phosphate isomerase Homo sapiens 45-48 8331595-11 1993 A decrease of extracellular buffer concentration (Hepes concentration lowered from 20 to 5 mM) caused an acidification of extracellular and intracellular pH and an acceleration of pHi recovery from alkalinization. HEPES 50-55 glucose-6-phosphate isomerase Homo sapiens 154-156 8331595-19 1993 The rate of pHi recovery from an alkaline load induced by changing from CO2-HCO3- to Hepes buffer was reduced by 33-56% in Cl(-)-free solution. co2-hco3 72-80 glucose-6-phosphate isomerase Homo sapiens 12-15 8331595-11 1993 A decrease of extracellular buffer concentration (Hepes concentration lowered from 20 to 5 mM) caused an acidification of extracellular and intracellular pH and an acceleration of pHi recovery from alkalinization. HEPES 50-55 glucose-6-phosphate isomerase Homo sapiens 180-183 8331595-19 1993 The rate of pHi recovery from an alkaline load induced by changing from CO2-HCO3- to Hepes buffer was reduced by 33-56% in Cl(-)-free solution. HEPES 85-90 glucose-6-phosphate isomerase Homo sapiens 12-15 8331595-15 1993 The H+ current blocker Zn2+ (0.5 mM) inhibited pHi recovery from alkalinization at resting membrane potential as well as during depolarization. Zinc 23-27 glucose-6-phosphate isomerase Homo sapiens 47-50 8331595-22 1993 The pHi recovery from alkalinization was DIDS-insensitive in CO2-HCO3(-)- as in Hepes-buffered solutions and was not slowed in the absence of external Na+. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 41-45 glucose-6-phosphate isomerase Homo sapiens 4-7 8331595-22 1993 The pHi recovery from alkalinization was DIDS-insensitive in CO2-HCO3(-)- as in Hepes-buffered solutions and was not slowed in the absence of external Na+. co2-hco3 61-69 glucose-6-phosphate isomerase Homo sapiens 4-7 8331595-22 1993 The pHi recovery from alkalinization was DIDS-insensitive in CO2-HCO3(-)- as in Hepes-buffered solutions and was not slowed in the absence of external Na+. HEPES 80-85 glucose-6-phosphate isomerase Homo sapiens 4-7 8331595-25 1993 In the presence of CO2-HCO3- buffer a DIDS-insensitive Cl(-)-HCO3- exchanger additionally regulates pHi after an intracellular alkaline load. co2-hco3 19-27 glucose-6-phosphate isomerase Homo sapiens 100-103 8331595-25 1993 In the presence of CO2-HCO3- buffer a DIDS-insensitive Cl(-)-HCO3- exchanger additionally regulates pHi after an intracellular alkaline load. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 38-42 glucose-6-phosphate isomerase Homo sapiens 100-103 8331595-18 1993 In Cl(-)-free, CO2-HCO3(-)-buffered solution pHi recovery from an alkaline load by changing from 5% CO2-27 mM HCO3- to 2% CO2-11 mM HCO3- was slowed by 48-71%. co2-hco3(-) 15-26 glucose-6-phosphate isomerase Homo sapiens 45-48 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). 13c 5-8 glucose-6-phosphate isomerase Homo sapiens 14-38 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). Glucose-6-Phosphate 204-223 glucose-6-phosphate isomerase Homo sapiens 14-38 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). 13c 5-8 glucose-6-phosphate isomerase Homo sapiens 40-43 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). Glucose-6-Phosphate 204-223 glucose-6-phosphate isomerase Homo sapiens 40-43 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). Glucose-6-Phosphate 225-228 glucose-6-phosphate isomerase Homo sapiens 14-38 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). mannose-6-phosphate 116-135 glucose-6-phosphate isomerase Homo sapiens 14-38 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). Glucose-6-Phosphate 225-228 glucose-6-phosphate isomerase Homo sapiens 40-43 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). fructose-6-phosphate 234-254 glucose-6-phosphate isomerase Homo sapiens 14-38 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). mannose-6-phosphate 116-135 glucose-6-phosphate isomerase Homo sapiens 40-43 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). fructose-6-phosphate 234-254 glucose-6-phosphate isomerase Homo sapiens 40-43 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). mannose-6-phosphate 137-140 glucose-6-phosphate isomerase Homo sapiens 14-38 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). fructose-6-phosphate 256-259 glucose-6-phosphate isomerase Homo sapiens 14-38 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). fructose-6-phosphate 256-259 glucose-6-phosphate isomerase Homo sapiens 40-43 8381960-1 1993 With 13C NMR, phosphoglucose isomerase (PGI; D-glucose-6-phosphate ketol-isomerase, EC 5.3.1.9) is shown to produce mannose 6-phosphate (M6P) slowly from a much more rapidly catalyzed equilibrium between glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P). mannose-6-phosphate 137-140 glucose-6-phosphate isomerase Homo sapiens 40-43 8381960-2 1993 The identity of M6P and its formation from G6P plus F6P are confirmed by 1H NMR and by the ability of PGI to convert M6P to F6P plus G6P. mannose-6-phosphate 16-19 glucose-6-phosphate isomerase Homo sapiens 102-105 8447421-2 1993 The present studies determined the effect of pHi on intracellular free Mg2+ activity in two epithelial cell lines: one possessing absorptive and secretory functions (Caco-2 intestinal cells) and the second, an absorptive kidney cell line derived from the opossum kidney (OK).pHi and spatial H+ distribution were assessed by 2",7"-bis(carboxyethyl)-5(6")-carboxyfluorescein after an NH4+ pulse to provide rapid changes in pHi. magnesium ion 71-75 glucose-6-phosphate isomerase Homo sapiens 45-48 8381960-4 1993 The pro-R and pro-S protons of F6P become the anomeric protons of M6P and G6P through the actions of PMI and PGI, respectively. Proline 4-7 glucose-6-phosphate isomerase Homo sapiens 109-112 8381960-4 1993 The pro-R and pro-S protons of F6P become the anomeric protons of M6P and G6P through the actions of PMI and PGI, respectively. Proline 14-17 glucose-6-phosphate isomerase Homo sapiens 109-112 8381960-4 1993 The pro-R and pro-S protons of F6P become the anomeric protons of M6P and G6P through the actions of PMI and PGI, respectively. Sulfur 18-19 glucose-6-phosphate isomerase Homo sapiens 109-112 8381960-5 1993 Both isomerases exchange the C2 proton of their substrate with the medium; hence, when PGI and PMI are added together to hexose phosphate solutions in 2H2O, both the substrate and anomeric protons are exchange rapidly with deuterons from the medium. Hexosephosphates 121-137 glucose-6-phosphate isomerase Homo sapiens 87-90 8381960-5 1993 Both isomerases exchange the C2 proton of their substrate with the medium; hence, when PGI and PMI are added together to hexose phosphate solutions in 2H2O, both the substrate and anomeric protons are exchange rapidly with deuterons from the medium. 2h2o 151-155 glucose-6-phosphate isomerase Homo sapiens 87-90 8381960-5 1993 Both isomerases exchange the C2 proton of their substrate with the medium; hence, when PGI and PMI are added together to hexose phosphate solutions in 2H2O, both the substrate and anomeric protons are exchange rapidly with deuterons from the medium. Deuterium 223-232 glucose-6-phosphate isomerase Homo sapiens 87-90 8381960-6 1993 The rates of C2-epimerization of G6P and M6P by PGI are shown to be proportional to enzyme concentration and inhibited by 5-phosphoarabinoate, a competitive inhibitor of the previously demonstrated isomerase and anomerase activities of PGI. 5-phosphoarabinoate 122-141 glucose-6-phosphate isomerase Homo sapiens 48-51 8381960-6 1993 The rates of C2-epimerization of G6P and M6P by PGI are shown to be proportional to enzyme concentration and inhibited by 5-phosphoarabinoate, a competitive inhibitor of the previously demonstrated isomerase and anomerase activities of PGI. 5-phosphoarabinoate 122-141 glucose-6-phosphate isomerase Homo sapiens 236-239 8381960-9 1993 A model of the mechanism of PGI is offered, which relates C2-epimerase activity to the isomerase and anomerase activities by allowing the cis-enediol intermediate to rotate about the C2-C3 bond axis followed by protonation at C2 but not at C1 from the si face. Silicon 252-254 glucose-6-phosphate isomerase Homo sapiens 28-31 8457847-3 1993 Derivation of intracellular pH (pHi) allowed calculation of "free" ADP (ADPf) and AMP (AMPf) concentrations. Adenosine Diphosphate 67-70 glucose-6-phosphate isomerase Homo sapiens 32-35 8457847-3 1993 Derivation of intracellular pH (pHi) allowed calculation of "free" ADP (ADPf) and AMP (AMPf) concentrations. adpf 72-76 glucose-6-phosphate isomerase Homo sapiens 32-35 8457847-3 1993 Derivation of intracellular pH (pHi) allowed calculation of "free" ADP (ADPf) and AMP (AMPf) concentrations. adenosine monophosphofluoridate 82-85 glucose-6-phosphate isomerase Homo sapiens 32-35 8457847-3 1993 Derivation of intracellular pH (pHi) allowed calculation of "free" ADP (ADPf) and AMP (AMPf) concentrations. adenosine monophosphofluoridate 87-91 glucose-6-phosphate isomerase Homo sapiens 32-35 8093745-6 1993 There were significant correlations (r > 0.6, p < 0.001) between markers of metabolic acidosis (base deficit in blood and extracellular fluid and bicarbonate concentration) and pHi. Bicarbonates 152-163 glucose-6-phosphate isomerase Homo sapiens 183-186 8093682-5 1993 Another two GABA malfunctioning connections were found in our monkey model: SNr-VA/VL and GPi-VA/VL. gamma-Aminobutyric Acid 12-16 glucose-6-phosphate isomerase Homo sapiens 90-93 8093682-7 1993 This chronic glutamatergic hyperactivity may have acted via an excitotoxic mechanism and consequently both GPi and VA/VL had a low synaptic activity in our dyskinetic monkeys, as measured by 2-deoxyglucose uptake, even 4 months after the last neuroleptic dose. Deoxyglucose 191-205 glucose-6-phosphate isomerase Homo sapiens 107-110 8457423-1 1993 Previous investigators have attributed the fall of brain intracellular pH (pHi) produced by ischemia to accumulation of lactic acid. Lactic Acid 120-131 glucose-6-phosphate isomerase Homo sapiens 75-78 7670541-3 1993 In epimastigote and metacyclic forms, the activity is found in the soluble fraction upon cell lysis, whereas in tissue cultured trypomastigotes it is membrane bound and, being mostly sensitive to p-chloromercuriphenylsulfonate, resembles closely the GPI specific phospholipase of Trypanosoma brucei. 4-Chloromercuribenzenesulfonate 196-226 glucose-6-phosphate isomerase Homo sapiens 250-253 7670541-11 1993 Previously unreported resistance to glycosylphosphatidylinositol-specific phospholipase D has been observed both to glycosylphosphatidylinositol-specific phospholipase D has been observed both to 1G7-Ag and 10D8-Ag, the GPI-anchored mucynlike protein which is acceptor of sialic acid in metacyclic forms. Glycosylphosphatidylinositols 36-64 glucose-6-phosphate isomerase Homo sapiens 220-223 7670541-11 1993 Previously unreported resistance to glycosylphosphatidylinositol-specific phospholipase D has been observed both to glycosylphosphatidylinositol-specific phospholipase D has been observed both to 1G7-Ag and 10D8-Ag, the GPI-anchored mucynlike protein which is acceptor of sialic acid in metacyclic forms. Glycosylphosphatidylinositols 116-144 glucose-6-phosphate isomerase Homo sapiens 220-223 7670541-11 1993 Previously unreported resistance to glycosylphosphatidylinositol-specific phospholipase D has been observed both to glycosylphosphatidylinositol-specific phospholipase D has been observed both to 1G7-Ag and 10D8-Ag, the GPI-anchored mucynlike protein which is acceptor of sialic acid in metacyclic forms. N-Acetylneuraminic Acid 272-283 glucose-6-phosphate isomerase Homo sapiens 220-223 27314787-2 1993 Much of the [1-(13)C]D-fructose 6-phosphate produced in this reaction was converted to [1-(13)C]D-glucose 6-phosphate by the addition of glucose-6-phosphate isomerase. [1-(13)c]d-fructose 6-phosphate 12-43 glucose-6-phosphate isomerase Homo sapiens 137-166 27314787-2 1993 Much of the [1-(13)C]D-fructose 6-phosphate produced in this reaction was converted to [1-(13)C]D-glucose 6-phosphate by the addition of glucose-6-phosphate isomerase. [1-(13)c]d-glucose 6-phosphate 87-117 glucose-6-phosphate isomerase Homo sapiens 137-166 8394427-5 1993 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) was employed in order to assess the mechanisms responsible for control of the pHi in an embryonic avian chondrocyte cell suspension. 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 33-100 glucose-6-phosphate isomerase Homo sapiens 16-18 8394427-5 1993 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) was employed in order to assess the mechanisms responsible for control of the pHi in an embryonic avian chondrocyte cell suspension. 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 33-100 glucose-6-phosphate isomerase Homo sapiens 190-193 8394427-5 1993 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) was employed in order to assess the mechanisms responsible for control of the pHi in an embryonic avian chondrocyte cell suspension. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 102-107 glucose-6-phosphate isomerase Homo sapiens 16-18 8394427-7 1993 Steady-state pHi in the absence of physiological HCO3- was 7.15 +/- 0.01 pH units as compared to a pHi of 6.94 +/- 0.02 pH units in its presence (P < 0.01). Bicarbonates 49-53 glucose-6-phosphate isomerase Homo sapiens 13-16 8394427-7 1993 Steady-state pHi in the absence of physiological HCO3- was 7.15 +/- 0.01 pH units as compared to a pHi of 6.94 +/- 0.02 pH units in its presence (P < 0.01). Bicarbonates 49-53 glucose-6-phosphate isomerase Homo sapiens 13-15 8394427-7 1993 Steady-state pHi in the absence of physiological HCO3- was 7.15 +/- 0.01 pH units as compared to a pHi of 6.94 +/- 0.02 pH units in its presence (P < 0.01). Bicarbonates 49-53 glucose-6-phosphate isomerase Homo sapiens 73-75 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. HEPES 32-37 glucose-6-phosphate isomerase Homo sapiens 116-119 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. HEPES 32-37 glucose-6-phosphate isomerase Homo sapiens 116-118 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. HEPES 32-37 glucose-6-phosphate isomerase Homo sapiens 151-153 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 64-67 glucose-6-phosphate isomerase Homo sapiens 116-119 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 64-67 glucose-6-phosphate isomerase Homo sapiens 116-118 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 64-67 glucose-6-phosphate isomerase Homo sapiens 151-153 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. Bicarbonates 74-79 glucose-6-phosphate isomerase Homo sapiens 116-119 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. Bicarbonates 74-79 glucose-6-phosphate isomerase Homo sapiens 116-118 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. Bicarbonates 74-79 glucose-6-phosphate isomerase Homo sapiens 151-153 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. Bicarbonates 74-78 glucose-6-phosphate isomerase Homo sapiens 116-119 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. Bicarbonates 74-78 glucose-6-phosphate isomerase Homo sapiens 116-118 8394427-16 1993 Chondrocytes transferred from a Hepes-buffered solution to a 5% CO2-25 mM HCO3- medium (HCO3- solution) underwent a pHi decrease of approximately 0.20 pH units, followed by a regulatory alkalinizing response of 0.118 pH units/min. Bicarbonates 74-78 glucose-6-phosphate isomerase Homo sapiens 151-153 1479478-1 1992 A new flow cytometric method was developed for measuring the intracellular pH (pHi) of mammalian cells using a fluorescent pH indicator dye 2",7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF). 2",7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein 140-193 glucose-6-phosphate isomerase Homo sapiens 79-82 8380078-6 1993 Synthesis of both gpI and gpIV included intermediary partially glycosylated forms and mature N- and O-linked final product. Nitrogen 93-94 glucose-6-phosphate isomerase Homo sapiens 18-21 8380078-7 1993 Transfections in the presence of 32Pi demonstrated that the mature forms of both gpI and gpIV were phosphorylated, while similar experiments with [35S]sulfate showed that only the mature gpI was sulfated. 32pi 33-37 glucose-6-phosphate isomerase Homo sapiens 81-84 8380078-7 1993 Transfections in the presence of 32Pi demonstrated that the mature forms of both gpI and gpIV were phosphorylated, while similar experiments with [35S]sulfate showed that only the mature gpI was sulfated. 32pi 33-37 glucose-6-phosphate isomerase Homo sapiens 89-92 1476167-3 1992 After acidification with nigericin, thrombin induced an acute increase of [Ca2+]i and a rise in pHi. Nigericin 25-34 glucose-6-phosphate isomerase Homo sapiens 96-99 1335727-6 1992 In HCO3(-)-free media, cells recovered from acid load by 0.34 +/- 0.04 pH units in the first 2 min and finally reached a pHi of 7.35 +/- 0.06. Bicarbonates 3-8 glucose-6-phosphate isomerase Homo sapiens 71-73 1335727-6 1992 In HCO3(-)-free media, cells recovered from acid load by 0.34 +/- 0.04 pH units in the first 2 min and finally reached a pHi of 7.35 +/- 0.06. Bicarbonates 3-8 glucose-6-phosphate isomerase Homo sapiens 121-124 1335727-8 1992 In HCO3(-)-containing media, cells recovered from an acid load at a similar rate, but reached 99 +/- 10% (n = 9) of the baseline pH; this recovery was also dependent on Na+ ions. Bicarbonates 3-10 glucose-6-phosphate isomerase Homo sapiens 129-131 1479478-1 1992 A new flow cytometric method was developed for measuring the intracellular pH (pHi) of mammalian cells using a fluorescent pH indicator dye 2",7-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF). bcecf 195-200 glucose-6-phosphate isomerase Homo sapiens 79-82 1522132-2 1992 In this work, the regulation of cell pH (pHi) was studied after addition or withdrawal of CO2/HCO3 (5% CO2, 95 mM HCO3, pH = 8) using the fluoroprobe BCECF. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 90-93 glucose-6-phosphate isomerase Homo sapiens 41-44 1331065-5 1992 Phorbol ester activation of acidified cells induced a rapid recovery of pHi partly due to a Zn(2+)-sensitive H(+)-conductive pathway. Phorbol Esters 0-13 glucose-6-phosphate isomerase Homo sapiens 72-75 1331065-5 1992 Phorbol ester activation of acidified cells induced a rapid recovery of pHi partly due to a Zn(2+)-sensitive H(+)-conductive pathway. Zinc 92-98 glucose-6-phosphate isomerase Homo sapiens 72-75 1331065-10 1992 The activity of the V-type H+ pumps was virtually undetectable in resting cells, becoming apparent only after treatment with phorbol esters or other, chemically unrelated agonists of protein kinase C. These H+ pumps are likely to play a role in pHi homeostasis during the metabolic burst that accompanies neutrophil activation during infection and inflammation. Phorbol Esters 125-139 glucose-6-phosphate isomerase Homo sapiens 245-248 1443100-6 1992 EIPA at 10 microM inhibited the Na+ influx, the rise in pHi, and intra- and extracellular IL-1 beta production in activated monocytes; this inhibition was reversed by 10 nM monensin. ethylisopropylamiloride 0-4 glucose-6-phosphate isomerase Homo sapiens 56-59 1443100-6 1992 EIPA at 10 microM inhibited the Na+ influx, the rise in pHi, and intra- and extracellular IL-1 beta production in activated monocytes; this inhibition was reversed by 10 nM monensin. Monensin 173-181 glucose-6-phosphate isomerase Homo sapiens 56-59 1443100-7 1992 In the absence of bicarbonate, or in the presence of 10 microM 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid, the pHi of activated monocytes and the total protein synthesis did not change, but the production of IL-1 beta was inhibited. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 63-111 glucose-6-phosphate isomerase Homo sapiens 117-120 1332693-3 1992 The basal pHi in HCO3(-)-free buffer was 7.36 +/- 0.04 units (mean +/- S.D.). Bicarbonates 17-22 glucose-6-phosphate isomerase Homo sapiens 10-13 1332693-4 1992 Addition of ionomycin in Ca(2+)-containing buffer did not cause a rise in basal pHi; however, addition of the phorbol ester phorbol 12-myristate 13-acetate (PMA) did cause a slowly developing rise in resting pHi of 0.14 +/- 0.02 unit over 4-5 min. Phorbol Esters 110-123 glucose-6-phosphate isomerase Homo sapiens 208-211 1332693-4 1992 Addition of ionomycin in Ca(2+)-containing buffer did not cause a rise in basal pHi; however, addition of the phorbol ester phorbol 12-myristate 13-acetate (PMA) did cause a slowly developing rise in resting pHi of 0.14 +/- 0.02 unit over 4-5 min. Tetradecanoylphorbol Acetate 124-155 glucose-6-phosphate isomerase Homo sapiens 208-211 1332693-4 1992 Addition of ionomycin in Ca(2+)-containing buffer did not cause a rise in basal pHi; however, addition of the phorbol ester phorbol 12-myristate 13-acetate (PMA) did cause a slowly developing rise in resting pHi of 0.14 +/- 0.02 unit over 4-5 min. Tetradecanoylphorbol Acetate 157-160 glucose-6-phosphate isomerase Homo sapiens 208-211 1332693-5 1992 Nigericin, a K+/H+ ionophore, caused an abrupt fall in pHi to 6.70 +/- 0.07 units. Nigericin 0-9 glucose-6-phosphate isomerase Homo sapiens 55-58 1332693-6 1992 In nigericin-pretreated cells, PMA caused a rapid rise in pHi without changing the [Ca2+]i. Nigericin 3-12 glucose-6-phosphate isomerase Homo sapiens 58-61 1332693-6 1992 In nigericin-pretreated cells, PMA caused a rapid rise in pHi without changing the [Ca2+]i. Tetradecanoylphorbol Acetate 31-34 glucose-6-phosphate isomerase Homo sapiens 58-61 1332693-7 1992 In acidified cells, ionomycin increased [Ca2+]i and pHi in a parallel concentration-dependent (30-500 nM) manner. Ionomycin 20-29 glucose-6-phosphate isomerase Homo sapiens 52-55 1332693-11 1992 Staurosporine, a protein kinase C inhibitor, blocked the action of PMA on pHi, but it had no effect on the ionomycin-induced increase in pHi. Staurosporine 0-13 glucose-6-phosphate isomerase Homo sapiens 74-77 1438704-1 1992 We report that coincubation of 647V cells for one cell cycle with low concentrations (30 microM) of 5"-amino-5"-deoxythymidine increased IdUrd DNA incorporation and radiosensitivity at low extracellular pH (pHe 6.8) in a fashion similar to treatment at normal pHe. 5'-amino-5'-deoxythymidine 100-126 glucose-6-phosphate isomerase Homo sapiens 203-205 1438704-1 1992 We report that coincubation of 647V cells for one cell cycle with low concentrations (30 microM) of 5"-amino-5"-deoxythymidine increased IdUrd DNA incorporation and radiosensitivity at low extracellular pH (pHe 6.8) in a fashion similar to treatment at normal pHe. Phenylalanine 207-210 glucose-6-phosphate isomerase Homo sapiens 203-205 1438704-5 1992 Flow cytometric measurement of pHi in 647V cells showed that normal pHi (pH 7.4) was maintained in 647V cells over a 12- to 24-h exposure to low pHe (pH 6.8). Phenylalanine 145-148 glucose-6-phosphate isomerase Homo sapiens 68-71 1438704-5 1992 Flow cytometric measurement of pHi in 647V cells showed that normal pHi (pH 7.4) was maintained in 647V cells over a 12- to 24-h exposure to low pHe (pH 6.8). Phenylalanine 145-148 glucose-6-phosphate isomerase Homo sapiens 68-70 1522132-2 1992 In this work, the regulation of cell pH (pHi) was studied after addition or withdrawal of CO2/HCO3 (5% CO2, 95 mM HCO3, pH = 8) using the fluoroprobe BCECF. Bicarbonates 94-98 glucose-6-phosphate isomerase Homo sapiens 41-44 1522132-2 1992 In this work, the regulation of cell pH (pHi) was studied after addition or withdrawal of CO2/HCO3 (5% CO2, 95 mM HCO3, pH = 8) using the fluoroprobe BCECF. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 103-106 glucose-6-phosphate isomerase Homo sapiens 41-44 1522132-2 1992 In this work, the regulation of cell pH (pHi) was studied after addition or withdrawal of CO2/HCO3 (5% CO2, 95 mM HCO3, pH = 8) using the fluoroprobe BCECF. Bicarbonates 114-118 glucose-6-phosphate isomerase Homo sapiens 41-44 1522132-3 1992 In the presence of Na and amiloride to inhibit Na/H exchange, the recovery of pHi after CO2 entry and CO2 exit were found to depend in part on HCO3 entry and exit, respectively. Amiloride 26-35 glucose-6-phosphate isomerase Homo sapiens 78-81 1522132-3 1992 In the presence of Na and amiloride to inhibit Na/H exchange, the recovery of pHi after CO2 entry and CO2 exit were found to depend in part on HCO3 entry and exit, respectively. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 88-91 glucose-6-phosphate isomerase Homo sapiens 78-81 1522132-3 1992 In the presence of Na and amiloride to inhibit Na/H exchange, the recovery of pHi after CO2 entry and CO2 exit were found to depend in part on HCO3 entry and exit, respectively. Bicarbonates 143-147 glucose-6-phosphate isomerase Homo sapiens 78-81 1522132-5 1992 DIDS, 0.5 mM, significantly inhibited both recovery phases of pHi. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 0-4 glucose-6-phosphate isomerase Homo sapiens 62-65 1522132-8 1992 Cell depolarization in the presence of Na moderately stimulated the pHi recovery rate after CO2 addition whereas it markedly inhibited the normalization of pHi after CO2 removal. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 92-95 glucose-6-phosphate isomerase Homo sapiens 68-71 1522132-8 1992 Cell depolarization in the presence of Na moderately stimulated the pHi recovery rate after CO2 addition whereas it markedly inhibited the normalization of pHi after CO2 removal. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 166-169 glucose-6-phosphate isomerase Homo sapiens 156-159 1522132-9 1992 Cell depolarization in the absence of sodium had only a slight effect to increase pHi recovery after CO2 addition but markedly prevented the pHi recovery after CO2 removal. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 160-163 glucose-6-phosphate isomerase Homo sapiens 141-144 1331976-6 1992 The inhibitory effects of SCH 28080 and amiloride were additive, demonstrating the involvement of a gastric-like H+/K(+)-ATPase and a Na+/H+ exchanger in regulating pHi. Sch 28080 26-35 glucose-6-phosphate isomerase Homo sapiens 165-168 1324151-9 1992 1,25-(OH)2D3 caused a dose-dependent decrease in pHi in CaCo-2 cells, as assessed by the fluorescent dye BCECF, which was not observed in cells suspended in Na(+)-free buffer or pretreated with amiloride, indicating that the secosteroid inhibited Na(+)-H+ exchange. Calcitriol 0-12 glucose-6-phosphate isomerase Homo sapiens 49-52 1324151-9 1992 1,25-(OH)2D3 caused a dose-dependent decrease in pHi in CaCo-2 cells, as assessed by the fluorescent dye BCECF, which was not observed in cells suspended in Na(+)-free buffer or pretreated with amiloride, indicating that the secosteroid inhibited Na(+)-H+ exchange. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 105-110 glucose-6-phosphate isomerase Homo sapiens 49-52 1324151-9 1992 1,25-(OH)2D3 caused a dose-dependent decrease in pHi in CaCo-2 cells, as assessed by the fluorescent dye BCECF, which was not observed in cells suspended in Na(+)-free buffer or pretreated with amiloride, indicating that the secosteroid inhibited Na(+)-H+ exchange. Amiloride 194-203 glucose-6-phosphate isomerase Homo sapiens 49-52 1324151-9 1992 1,25-(OH)2D3 caused a dose-dependent decrease in pHi in CaCo-2 cells, as assessed by the fluorescent dye BCECF, which was not observed in cells suspended in Na(+)-free buffer or pretreated with amiloride, indicating that the secosteroid inhibited Na(+)-H+ exchange. Secosteroids 225-236 glucose-6-phosphate isomerase Homo sapiens 49-52 1331972-11 1992 These experiments suggest that the target for the human calcitonin effect on pHi is the Cl-/HCO3- exchanger. Bicarbonates 92-96 glucose-6-phosphate isomerase Homo sapiens 77-80 1331976-4 1992 Control pHi, measured in HCO(3-)-free medium, was 7.62 +/- 0.03 (n = 27) when cells were cultured for 14 days and decreased to 7.40 +/- 0.03 (n = 18) after 35 days in culture. Bicarbonates 25-32 glucose-6-phosphate isomerase Homo sapiens 8-11 1331976-6 1992 The inhibitory effects of SCH 28080 and amiloride were additive, demonstrating the involvement of a gastric-like H+/K(+)-ATPase and a Na+/H+ exchanger in regulating pHi. Amiloride 40-49 glucose-6-phosphate isomerase Homo sapiens 165-168 1331976-5 1992 Recovery of pHi following a NH+4/NH3 pulse could be reduced by either 100 microM SCH 28080 or 1 mM amiloride, or by removing extracellular Na+. Sch 28080 81-90 glucose-6-phosphate isomerase Homo sapiens 12-15 1331976-5 1992 Recovery of pHi following a NH+4/NH3 pulse could be reduced by either 100 microM SCH 28080 or 1 mM amiloride, or by removing extracellular Na+. Amiloride 99-108 glucose-6-phosphate isomerase Homo sapiens 12-15 1331976-9 1992 Recovery of pHi was also inhibited by 1 mM N-ethylmaleimide. Ethylmaleimide 43-59 glucose-6-phosphate isomerase Homo sapiens 12-15 1331976-11 1992 In conclusion, Caco-2 cells contain a SCH-28080-sensitive mechanism for regulating pHi, which is most conveniently studied after 28 days in culture, when the relative contribution of a Na+/H+ exchanger to pHi regulation is decreased. Sch 28080 38-47 glucose-6-phosphate isomerase Homo sapiens 83-86 1331976-11 1992 In conclusion, Caco-2 cells contain a SCH-28080-sensitive mechanism for regulating pHi, which is most conveniently studied after 28 days in culture, when the relative contribution of a Na+/H+ exchanger to pHi regulation is decreased. Sch 28080 38-47 glucose-6-phosphate isomerase Homo sapiens 205-208 1325517-6 1992 METHODS: Platelets were loaded with the intracellular pH (pHi) indicator 2"-7"-bis-carboxyethyl-5(6)-carboxyfluorescein (BCECF) and acidified by propionic acid. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 73-119 glucose-6-phosphate isomerase Homo sapiens 58-61 1322790-1 1992 The effect of microenvironmental factors on the regulation of intracellular pH (pHi) in MGH U1 cells and EMT-6 cells was studied using the fluorescent pH probe 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein. 2",7"-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein 160-215 glucose-6-phosphate isomerase Homo sapiens 80-83 1322790-5 1992 This effect was more marked for the Na(+)-dependent Cl-/HCO3- exchanger than for the Na+/H+ antiporter, suggesting that under conditions of reduced pHe the Na+/H+ antiporter is the major mechanism for regulation of pHi. Phenylalanine 148-151 glucose-6-phosphate isomerase Homo sapiens 215-218 1500883-2 1992 Liver, in common with many tissues, has plasma membrane Na(+)-H+ and Cl(-)-HCO3- electroneutral exchangers which work in opposition to tightly control pHi. Bicarbonates 75-79 glucose-6-phosphate isomerase Homo sapiens 151-154 1322838-1 1992 BCECF fluorescence has been applied to determine intracellular pH (pHi) in NIH 3T3 fibroblasts expressing the Ha-ras oncogene (+ras) and otherwise identical cells not expressing the oncogene (-ras). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 0-5 glucose-6-phosphate isomerase Homo sapiens 67-70 1324676-1 1992 The fluorescent pH probe, 2"-7"-bis (carboxyethyl) 5-carboxyfluorescein, was used to follow changes in internal pH (pHi) induced by aromatic polyene antibiotics in the BALB/c lymphoid cell line A20. 2"-7"-bis (carboxyethyl) 5-carboxyfluorescein 26-71 glucose-6-phosphate isomerase Homo sapiens 116-119 1324676-1 1992 The fluorescent pH probe, 2"-7"-bis (carboxyethyl) 5-carboxyfluorescein, was used to follow changes in internal pH (pHi) induced by aromatic polyene antibiotics in the BALB/c lymphoid cell line A20. aromatic 132-140 glucose-6-phosphate isomerase Homo sapiens 116-119 1324676-1 1992 The fluorescent pH probe, 2"-7"-bis (carboxyethyl) 5-carboxyfluorescein, was used to follow changes in internal pH (pHi) induced by aromatic polyene antibiotics in the BALB/c lymphoid cell line A20. polyene antibiotics 141-160 glucose-6-phosphate isomerase Homo sapiens 116-119 1325517-6 1992 METHODS: Platelets were loaded with the intracellular pH (pHi) indicator 2"-7"-bis-carboxyethyl-5(6)-carboxyfluorescein (BCECF) and acidified by propionic acid. propionic acid 145-159 glucose-6-phosphate isomerase Homo sapiens 58-61 1623750-5 1992 In the dobutamine low pHi group, pHi increased significantly from 7.16 +/- 0.03 to 7.24 +/- 0.03 (n = 9, p less than 0.05). Dobutamine 7-17 glucose-6-phosphate isomerase Homo sapiens 22-25 1623750-5 1992 In the dobutamine low pHi group, pHi increased significantly from 7.16 +/- 0.03 to 7.24 +/- 0.03 (n = 9, p less than 0.05). Dobutamine 7-17 glucose-6-phosphate isomerase Homo sapiens 33-36 1408660-3 1992 In order to prevent regulation of pHi, Na+ ions were replaced with N-methyl-D-glucamine. Meglumine 67-87 glucose-6-phosphate isomerase Homo sapiens 34-37 1408660-7 1992 A more prolonged application of BA and AA at concentrations of 10(-7) M and higher slowed the CO2-induced fall in pHi, which attained a rate corresponding to uncatalysed intracellular CO2 hydration at an AA concentration of 10(-4) M. The effect of BA and AA on the pHi changes developed with a time constant of 25 +/- 4 min and 7.6 +/- 1.5 min respectively, indicating that BA is less permeant than AA. Benzolamide 32-34 glucose-6-phosphate isomerase Homo sapiens 114-117 1408660-7 1992 A more prolonged application of BA and AA at concentrations of 10(-7) M and higher slowed the CO2-induced fall in pHi, which attained a rate corresponding to uncatalysed intracellular CO2 hydration at an AA concentration of 10(-4) M. The effect of BA and AA on the pHi changes developed with a time constant of 25 +/- 4 min and 7.6 +/- 1.5 min respectively, indicating that BA is less permeant than AA. Benzolamide 32-34 glucose-6-phosphate isomerase Homo sapiens 265-268 1408660-7 1992 A more prolonged application of BA and AA at concentrations of 10(-7) M and higher slowed the CO2-induced fall in pHi, which attained a rate corresponding to uncatalysed intracellular CO2 hydration at an AA concentration of 10(-4) M. The effect of BA and AA on the pHi changes developed with a time constant of 25 +/- 4 min and 7.6 +/- 1.5 min respectively, indicating that BA is less permeant than AA. Benzolamide 248-250 glucose-6-phosphate isomerase Homo sapiens 114-117 1408660-7 1992 A more prolonged application of BA and AA at concentrations of 10(-7) M and higher slowed the CO2-induced fall in pHi, which attained a rate corresponding to uncatalysed intracellular CO2 hydration at an AA concentration of 10(-4) M. The effect of BA and AA on the pHi changes developed with a time constant of 25 +/- 4 min and 7.6 +/- 1.5 min respectively, indicating that BA is less permeant than AA. Benzolamide 248-250 glucose-6-phosphate isomerase Homo sapiens 114-117 1319688-1 1992 The pH-sensitive probe 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein was used to measure intracellular pH (pHi) and test for Na(+)-H+ exchange in single ciliated human nasal epithelial (HNE) cells from normal and cystic fibrosis (CF) subjects. 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein 23-72 glucose-6-phosphate isomerase Homo sapiens 111-114 1320038-3 1992 Pretreatment with amiloride (0.3 mM) mostly blocked dbcAMP- and Sp-cAMPS-induced decrease in pHi but did not affect calcium ionophore-induced decrease in pHi. Amiloride 18-27 glucose-6-phosphate isomerase Homo sapiens 93-96 1320038-3 1992 Pretreatment with amiloride (0.3 mM) mostly blocked dbcAMP- and Sp-cAMPS-induced decrease in pHi but did not affect calcium ionophore-induced decrease in pHi. Bucladesine 52-58 glucose-6-phosphate isomerase Homo sapiens 93-96 1320038-3 1992 Pretreatment with amiloride (0.3 mM) mostly blocked dbcAMP- and Sp-cAMPS-induced decrease in pHi but did not affect calcium ionophore-induced decrease in pHi. adenosine-3',5'-cyclic phosphorothioate 64-72 glucose-6-phosphate isomerase Homo sapiens 93-96 1320038-4 1992 In the presence of amiloride, PTH and PTHrP caused a transient decrease in pHi, which was similar to the pattern of calcium ionophore-induced change in pHi. Amiloride 19-28 glucose-6-phosphate isomerase Homo sapiens 75-78 1320038-4 1992 In the presence of amiloride, PTH and PTHrP caused a transient decrease in pHi, which was similar to the pattern of calcium ionophore-induced change in pHi. Amiloride 19-28 glucose-6-phosphate isomerase Homo sapiens 152-155 1320038-4 1992 In the presence of amiloride, PTH and PTHrP caused a transient decrease in pHi, which was similar to the pattern of calcium ionophore-induced change in pHi. Calcium 116-123 glucose-6-phosphate isomerase Homo sapiens 75-78 1320038-4 1992 In the presence of amiloride, PTH and PTHrP caused a transient decrease in pHi, which was similar to the pattern of calcium ionophore-induced change in pHi. Calcium 116-123 glucose-6-phosphate isomerase Homo sapiens 152-155 1319688-2 1992 In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered NaCl Ringer solution, average pHi for normal and CF ciliated HNE cells was 7.15 +/- 0.02 and 7.18 +/- 0.03, respectively. HEPES 3-54 glucose-6-phosphate isomerase Homo sapiens 102-105 1319688-2 1992 In N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered NaCl Ringer solution, average pHi for normal and CF ciliated HNE cells was 7.15 +/- 0.02 and 7.18 +/- 0.03, respectively. HEPES 56-61 glucose-6-phosphate isomerase Homo sapiens 102-105 1319688-3 1992 Utilizing the NH+4-loading technique to acid load cells, we found that pHi decreased from approximately 7.2 to approximately 6.6; subsequent alkalinization of normal and CF ciliated cells required Na+ and was independent of Cl- but was blocked by amiloride (500 microM). Amiloride 247-256 glucose-6-phosphate isomerase Homo sapiens 71-74 1356225-1 1992 The introduction of exogenous glutamate to normally respiring hippocampal slices produced substantial reductions in ATP, phosphocreatine (PCr) and intracellular pH (pHi) when the concentration exceeded 1 mM. Glutamic Acid 30-39 glucose-6-phosphate isomerase Homo sapiens 165-168 1629081-9 1992 The results indicated that chemosensory responses to brief ischemia and hypoxia were not mediated by a fall of pHi of CB cells, whereas those to CO2 and extracellular acidity were associated with decreases in pHi. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 145-148 glucose-6-phosphate isomerase Homo sapiens 209-212 1570347-2 1992 In individual cells, membrane depolarization produced by transient exposure to 50 mM K+ caused a reversible increase in pHi in the presence, but not absence, of HCO3-, consistent with voltage-dependent HCO3- influx. Bicarbonates 202-206 glucose-6-phosphate isomerase Homo sapiens 120-123 1322994-0 1992 Na(+)-dependent HCO3- transport and Na+/H+ exchange regulate pHi in human ciliary muscle cells. Bicarbonates 16-20 glucose-6-phosphate isomerase Homo sapiens 61-64 1322994-1 1992 We investigated intracellular pH (pHi) regulation in cultured human ciliary muscle cells by means of the pH-sensitive absorbance of 5(and 6)-carboxy-4",5"-dimethylfluorescein (CDMF). 5(and 6)-carboxy-4",5"-dimethylfluorescein 132-174 glucose-6-phosphate isomerase Homo sapiens 34-37 1322994-1 1992 We investigated intracellular pH (pHi) regulation in cultured human ciliary muscle cells by means of the pH-sensitive absorbance of 5(and 6)-carboxy-4",5"-dimethylfluorescein (CDMF). cdmf 176-180 glucose-6-phosphate isomerase Homo sapiens 34-37 1322994-2 1992 The steady-state pHi was 7.09 +/- 0.04 (n = 12) in CO2/HCO3(-)-buffered and 6.86 +/- 0.03 (n = 12) in HEPES-buffered solution. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 51-54 glucose-6-phosphate isomerase Homo sapiens 17-20 1322994-4 1992 Readdition of external sodium resulted in a rapid pHi recovery, which was almost completely amiloride-sensitive in the absence of CO2/HCO3- but only slightly influenced by amiloride in its presence. Sodium 23-29 glucose-6-phosphate isomerase Homo sapiens 50-53 1322994-4 1992 Readdition of external sodium resulted in a rapid pHi recovery, which was almost completely amiloride-sensitive in the absence of CO2/HCO3- but only slightly influenced by amiloride in its presence. Amiloride 92-101 glucose-6-phosphate isomerase Homo sapiens 50-53 1322994-4 1992 Readdition of external sodium resulted in a rapid pHi recovery, which was almost completely amiloride-sensitive in the absence of CO2/HCO3- but only slightly influenced by amiloride in its presence. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 130-133 glucose-6-phosphate isomerase Homo sapiens 50-53 1322994-4 1992 Readdition of external sodium resulted in a rapid pHi recovery, which was almost completely amiloride-sensitive in the absence of CO2/HCO3- but only slightly influenced by amiloride in its presence. Bicarbonates 134-138 glucose-6-phosphate isomerase Homo sapiens 50-53 1322994-4 1992 Readdition of external sodium resulted in a rapid pHi recovery, which was almost completely amiloride-sensitive in the absence of CO2/HCO3- but only slightly influenced by amiloride in its presence. Amiloride 172-181 glucose-6-phosphate isomerase Homo sapiens 50-53 1322994-6 1992 The pHi recovery after an intracellular acid load was completely dependent on extracellular sodium. Sodium 92-98 glucose-6-phosphate isomerase Homo sapiens 4-7 1322994-7 1992 In HEPES-buffered solution the pHi recovery was almost completely mediated by Na+/H+ exchange, since it was blocked by amiloride (1 mmol/liter). HEPES 3-8 glucose-6-phosphate isomerase Homo sapiens 31-34 1322994-7 1992 In HEPES-buffered solution the pHi recovery was almost completely mediated by Na+/H+ exchange, since it was blocked by amiloride (1 mmol/liter). Amiloride 119-128 glucose-6-phosphate isomerase Homo sapiens 31-34 1322994-8 1992 In contrast, a marked amiloride-insensitive pHi recovery was observed in CO2/HCO3(-)-buffered solution which was mediated by chloride-independent and chloride-dependent Na+ HCO3- cotransport. Amiloride 22-31 glucose-6-phosphate isomerase Homo sapiens 44-47 1322994-8 1992 In contrast, a marked amiloride-insensitive pHi recovery was observed in CO2/HCO3(-)-buffered solution which was mediated by chloride-independent and chloride-dependent Na+ HCO3- cotransport. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 73-76 glucose-6-phosphate isomerase Homo sapiens 44-47 1322994-8 1992 In contrast, a marked amiloride-insensitive pHi recovery was observed in CO2/HCO3(-)-buffered solution which was mediated by chloride-independent and chloride-dependent Na+ HCO3- cotransport. Bicarbonates 77-84 glucose-6-phosphate isomerase Homo sapiens 44-47 1322994-8 1992 In contrast, a marked amiloride-insensitive pHi recovery was observed in CO2/HCO3(-)-buffered solution which was mediated by chloride-independent and chloride-dependent Na+ HCO3- cotransport. Chlorides 125-133 glucose-6-phosphate isomerase Homo sapiens 44-47 1322994-10 1992 Analysis of the sodium dependence of the pHi recovery after NH4Cl prepulse revealed Vmax = 0.57 pH units/min, Km = 39.7 mmol/liter extracellular sodium for the amiloride-sensitive component and Vmax = 0.19 pH units/min, Km = 14.3 mmol/liter extracellular sodium for the amiloride-insensitive component. Sodium 16-22 glucose-6-phosphate isomerase Homo sapiens 41-44 1322994-10 1992 Analysis of the sodium dependence of the pHi recovery after NH4Cl prepulse revealed Vmax = 0.57 pH units/min, Km = 39.7 mmol/liter extracellular sodium for the amiloride-sensitive component and Vmax = 0.19 pH units/min, Km = 14.3 mmol/liter extracellular sodium for the amiloride-insensitive component. Ammonium Chloride 60-65 glucose-6-phosphate isomerase Homo sapiens 41-44 1322994-10 1992 Analysis of the sodium dependence of the pHi recovery after NH4Cl prepulse revealed Vmax = 0.57 pH units/min, Km = 39.7 mmol/liter extracellular sodium for the amiloride-sensitive component and Vmax = 0.19 pH units/min, Km = 14.3 mmol/liter extracellular sodium for the amiloride-insensitive component. Sodium 145-151 glucose-6-phosphate isomerase Homo sapiens 41-44 1322994-10 1992 Analysis of the sodium dependence of the pHi recovery after NH4Cl prepulse revealed Vmax = 0.57 pH units/min, Km = 39.7 mmol/liter extracellular sodium for the amiloride-sensitive component and Vmax = 0.19 pH units/min, Km = 14.3 mmol/liter extracellular sodium for the amiloride-insensitive component. Amiloride 160-169 glucose-6-phosphate isomerase Homo sapiens 41-44 1322994-10 1992 Analysis of the sodium dependence of the pHi recovery after NH4Cl prepulse revealed Vmax = 0.57 pH units/min, Km = 39.7 mmol/liter extracellular sodium for the amiloride-sensitive component and Vmax = 0.19 pH units/min, Km = 14.3 mmol/liter extracellular sodium for the amiloride-insensitive component. Sodium 145-151 glucose-6-phosphate isomerase Homo sapiens 41-44 1322994-10 1992 Analysis of the sodium dependence of the pHi recovery after NH4Cl prepulse revealed Vmax = 0.57 pH units/min, Km = 39.7 mmol/liter extracellular sodium for the amiloride-sensitive component and Vmax = 0.19 pH units/min, Km = 14.3 mmol/liter extracellular sodium for the amiloride-insensitive component. Amiloride 270-279 glucose-6-phosphate isomerase Homo sapiens 41-44 1322994-11 1992 We conclude that Na+/H+ exchange and chloride-independent and chloride-dependent Na(+)-HCO3- cotransport are involved in the pHi regulation of cultured human ciliary muscle cells. Chlorides 37-45 glucose-6-phosphate isomerase Homo sapiens 125-128 1591268-3 1992 The set point of control platelets (7.28 +/- 0.01) is shifted rapidly (discernibly less than or equal to 30 s) and markedly to alkaline pHi (7.62 +/- 0.03) by PMA, that activates protein kinase C and is shifted to acidic pHi (7.05 +/- 0.01) by staurosporine, which inhibits protein kinases. Tetradecanoylphorbol Acetate 159-162 glucose-6-phosphate isomerase Homo sapiens 136-139 1591268-3 1992 The set point of control platelets (7.28 +/- 0.01) is shifted rapidly (discernibly less than or equal to 30 s) and markedly to alkaline pHi (7.62 +/- 0.03) by PMA, that activates protein kinase C and is shifted to acidic pHi (7.05 +/- 0.01) by staurosporine, which inhibits protein kinases. Tetradecanoylphorbol Acetate 159-162 glucose-6-phosphate isomerase Homo sapiens 221-224 1591268-3 1992 The set point of control platelets (7.28 +/- 0.01) is shifted rapidly (discernibly less than or equal to 30 s) and markedly to alkaline pHi (7.62 +/- 0.03) by PMA, that activates protein kinase C and is shifted to acidic pHi (7.05 +/- 0.01) by staurosporine, which inhibits protein kinases. Staurosporine 244-257 glucose-6-phosphate isomerase Homo sapiens 136-139 1316474-7 1992 Like the wild-type form of gpI expressed in VZV-infected cells, gpI precipitated from transfected cells contained both N-linked and O-linked glycans and was heavily sialated. n-linked and o-linked glycans 119-148 glucose-6-phosphate isomerase Homo sapiens 64-67 1570347-3 1992 In the absence of HCO3-, intracellular alkalinization and acidification produced by NH4Cl exposure and withdrawal produced membrane hyperpolarization and depolarization, respectively, as expected for pHi-induced changes in gK+. Ammonium Chloride 84-89 glucose-6-phosphate isomerase Homo sapiens 200-203 1570347-5 1992 Moreover, the rate of pHi and potential difference recovery was several-fold greater in the presence as compared with the absence of HCO3-. Bicarbonates 133-137 glucose-6-phosphate isomerase Homo sapiens 22-25 1570347-6 1992 Finally, continuous exposure to 10% CO2 in the presence of HCO3- produced intracellular acidification, and the rate of pHi recovery from intracellular acidosis was inhibited by Ba2+, which blocks pHi-induced changes in gK+, and by 4-acetamido-4"-isothiocyanatostilbene-2,2"-disulfonic acid, which inhibits Na+/HCO3- cotransport. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 36-39 glucose-6-phosphate isomerase Homo sapiens 196-199 1570347-6 1992 Finally, continuous exposure to 10% CO2 in the presence of HCO3- produced intracellular acidification, and the rate of pHi recovery from intracellular acidosis was inhibited by Ba2+, which blocks pHi-induced changes in gK+, and by 4-acetamido-4"-isothiocyanatostilbene-2,2"-disulfonic acid, which inhibits Na+/HCO3- cotransport. Bicarbonates 59-63 glucose-6-phosphate isomerase Homo sapiens 196-199 1570347-6 1992 Finally, continuous exposure to 10% CO2 in the presence of HCO3- produced intracellular acidification, and the rate of pHi recovery from intracellular acidosis was inhibited by Ba2+, which blocks pHi-induced changes in gK+, and by 4-acetamido-4"-isothiocyanatostilbene-2,2"-disulfonic acid, which inhibits Na+/HCO3- cotransport. N-methyl-valyl-amiclenomycin 177-181 glucose-6-phosphate isomerase Homo sapiens 119-122 1570347-6 1992 Finally, continuous exposure to 10% CO2 in the presence of HCO3- produced intracellular acidification, and the rate of pHi recovery from intracellular acidosis was inhibited by Ba2+, which blocks pHi-induced changes in gK+, and by 4-acetamido-4"-isothiocyanatostilbene-2,2"-disulfonic acid, which inhibits Na+/HCO3- cotransport. N-methyl-valyl-amiclenomycin 177-181 glucose-6-phosphate isomerase Homo sapiens 196-199 1570347-6 1992 Finally, continuous exposure to 10% CO2 in the presence of HCO3- produced intracellular acidification, and the rate of pHi recovery from intracellular acidosis was inhibited by Ba2+, which blocks pHi-induced changes in gK+, and by 4-acetamido-4"-isothiocyanatostilbene-2,2"-disulfonic acid, which inhibits Na+/HCO3- cotransport. 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 231-289 glucose-6-phosphate isomerase Homo sapiens 119-122 1570347-6 1992 Finally, continuous exposure to 10% CO2 in the presence of HCO3- produced intracellular acidification, and the rate of pHi recovery from intracellular acidosis was inhibited by Ba2+, which blocks pHi-induced changes in gK+, and by 4-acetamido-4"-isothiocyanatostilbene-2,2"-disulfonic acid, which inhibits Na+/HCO3- cotransport. 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 231-289 glucose-6-phosphate isomerase Homo sapiens 196-199 1539631-7 1992 5) pHi backregulation after an acid load occurred in both the presence and absence of extracellular bicarbonate but not in the absence of extracellular sodium. Bicarbonates 100-111 glucose-6-phosphate isomerase Homo sapiens 3-6 1324804-4 1992 Signal transduction following binding of AMF to its receptor, a cell surface glycoprotein of 78 kD (gp78) homologous to p53, is mediated by a pertussis toxin sensitive G protein, inositol phosphate production and the phosphorylation of gp78. Inositol Phosphates 179-197 glucose-6-phosphate isomerase Homo sapiens 41-44 1588931-10 1992 Similarly GSH was reduced (p less than 0.05) in EH (Gp IIA 11.2 +/- 1.7 mg per gm protein, Gp IIB 8.5 +/- 1.1 and Gp IIC 6.6 +/- 0.3) as compared to Gp I (13.5 +/- 2.5). Glutathione 10-13 glucose-6-phosphate isomerase Homo sapiens 52-56 1311273-3 1992 Subsequent removal of HCO3-/CO2 (HEPES/O2 substitution) from the serosal perfusate caused a further decrease of pHi. Bicarbonates 22-26 glucose-6-phosphate isomerase Homo sapiens 112-115 1311273-3 1992 Subsequent removal of HCO3-/CO2 (HEPES/O2 substitution) from the serosal perfusate caused a further decrease of pHi. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 28-31 glucose-6-phosphate isomerase Homo sapiens 112-115 1311273-3 1992 Subsequent removal of HCO3-/CO2 (HEPES/O2 substitution) from the serosal perfusate caused a further decrease of pHi. HEPES 33-38 glucose-6-phosphate isomerase Homo sapiens 112-115 1311273-3 1992 Subsequent removal of HCO3-/CO2 (HEPES/O2 substitution) from the serosal perfusate caused a further decrease of pHi. Oxygen 29-31 glucose-6-phosphate isomerase Homo sapiens 112-115 1311273-4 1992 Blocking of HCO3- transport across the basolateral cell membrane by addition of 4-acetamido-4,isothiosyanostilbene-2,2-disulfonic acid (SITS) to serosal perfusate also caused a slight but significant decrease of pHi. Bicarbonates 12-16 glucose-6-phosphate isomerase Homo sapiens 212-215 1311273-4 1992 Blocking of HCO3- transport across the basolateral cell membrane by addition of 4-acetamido-4,isothiosyanostilbene-2,2-disulfonic acid (SITS) to serosal perfusate also caused a slight but significant decrease of pHi. 4-acetamido-4,isothiosyanostilbene-2,2-disulfonic acid 80-134 glucose-6-phosphate isomerase Homo sapiens 212-215 1311273-5 1992 Removal of Na+ (choline substitution) from the serosal perfusate during acid exposure likewise caused a significant decrease in pHi, as did serosal addition of an inhibitor of Na+/H+ antiport, 1 mmol/L amiloride. Choline 16-23 glucose-6-phosphate isomerase Homo sapiens 128-131 1311273-9 1992 Removal of serosal HCO3-/CO2 decreased surface pH significantly both at the villus apex and at the cryptal area, suggesting that the surface alkalization is mediated by transport of serosal HCO3- to the epithelial surface. Bicarbonates 19-23 glucose-6-phosphate isomerase Homo sapiens 47-49 1311273-9 1992 Removal of serosal HCO3-/CO2 decreased surface pH significantly both at the villus apex and at the cryptal area, suggesting that the surface alkalization is mediated by transport of serosal HCO3- to the epithelial surface. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 25-28 glucose-6-phosphate isomerase Homo sapiens 47-49 1311273-9 1992 Removal of serosal HCO3-/CO2 decreased surface pH significantly both at the villus apex and at the cryptal area, suggesting that the surface alkalization is mediated by transport of serosal HCO3- to the epithelial surface. Bicarbonates 190-194 glucose-6-phosphate isomerase Homo sapiens 47-49 1311273-10 1992 The data suggest that pHi in acid-exposed duodenal mucosa is primarily maintained within physiological range by an HCO3(-)-dependent mechanism, which, at least in part, exerts its action extracellularly by forming an alkaline buffer layer at the epithelial surface. Bicarbonates 115-122 glucose-6-phosphate isomerase Homo sapiens 22-25 1311273-11 1992 If adequate serosal (or systemic) HCO3- is not available, a second-line Na(+)-dependent and amiloride-sensitive pHi-regulatory mechanism, presumably an Na+/H+ antiport, becomes the main regulator of pHi. Bicarbonates 34-38 glucose-6-phosphate isomerase Homo sapiens 112-115 1311273-11 1992 If adequate serosal (or systemic) HCO3- is not available, a second-line Na(+)-dependent and amiloride-sensitive pHi-regulatory mechanism, presumably an Na+/H+ antiport, becomes the main regulator of pHi. Amiloride 92-101 glucose-6-phosphate isomerase Homo sapiens 112-115 1311273-11 1992 If adequate serosal (or systemic) HCO3- is not available, a second-line Na(+)-dependent and amiloride-sensitive pHi-regulatory mechanism, presumably an Na+/H+ antiport, becomes the main regulator of pHi. Amiloride 92-101 glucose-6-phosphate isomerase Homo sapiens 199-202 1735251-6 1992 With AE, there was a significant increase in Pg at end inspiration (PgI, 15.4 +/- 3.2 vs 11.9 +/- 2.7 cm H2O, p less than 0.01) and in Pdi (26.5 +/- 3.4 vs 21.4 +/- 2.4 cm H2O, p less than 0.01) with no change in Pg at end expiration (PgE) or in Ppl. pg 45-47 glucose-6-phosphate isomerase Homo sapiens 68-71 1346170-11 1992 pHi-guided resuscitation may help improve outcome in such patients by preventing splanchnic organ hypoxia and the development of a systemic oxygen deficit. Oxygen 140-146 glucose-6-phosphate isomerase Homo sapiens 0-3 1733231-3 1992 Resting pHi averaged 7.15 +/- 0.03 and was unaffected by the nominal removal of medium HCO3- or by the addition of the anion-exchange inhibitor 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS) but was significantly reduced by amiloride (7.07 +/- 0.02). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 144-192 glucose-6-phosphate isomerase Homo sapiens 8-11 1488862-1 1992 A therapeutic trial with mannose given intravenously as a 5% solution during 7 consecutive days (daily doses 12.5 g, 25 g and 50 g) was performed in a GPI deficient girl with nonspherocytic hemolytic anemia. Mannose 25-32 glucose-6-phosphate isomerase Homo sapiens 151-154 1310229-4 1992 At a luminal perfusate [HCO3] of 25 mM, progressive increases in luminal flow rate (5----15----25----40 nl/min) caused progressive increases in pHi. Bicarbonates 24-28 glucose-6-phosphate isomerase Homo sapiens 144-147 1310229-7 1992 The activity of the apical membrane Na-H antiporter, assayed as the initial rate of pHi recovery from an acid load in the presence of peritubular DIDS, was faster at 40 compared with 5 nl/min. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 146-150 glucose-6-phosphate isomerase Homo sapiens 84-87 1733231-6 1992 Recovery of pHi was also aided by HCO3(-)-dependent and DIDS-sensitive mechanisms not seen in the resting cell. Bicarbonates 34-38 glucose-6-phosphate isomerase Homo sapiens 12-15 1733231-1 1992 The fluorescent pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)- carboxyfluorescein (BCECF) was used to determine changes in intracellular pH (pHi) associated with activation of secretion in isolated cells from the salt-secreting avian nasal gland. 2",7"-bis(2-carboxyethyl)-5(6)- carboxyfluorescein 33-83 glucose-6-phosphate isomerase Homo sapiens 143-146 1733231-1 1992 The fluorescent pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)- carboxyfluorescein (BCECF) was used to determine changes in intracellular pH (pHi) associated with activation of secretion in isolated cells from the salt-secreting avian nasal gland. bcecf 85-90 glucose-6-phosphate isomerase Homo sapiens 143-146 1310229-8 1992 Basolateral membrane Na-3HCO3 symporter activity, assayed as the initial rate of pHi recovery from an alkali load in the absence of luminal and peritubular chloride, was faster at 40 compared with 5 nl/min. 3hco3 24-29 glucose-6-phosphate isomerase Homo sapiens 81-84 1310229-9 1992 This effect was eliminated by luminal amiloride, suggesting an indirect effect of flow mediated by changes in pHi secondary to flow rate-dependent changes in apical membrane Na-H antiporter activity. Amiloride 38-47 glucose-6-phosphate isomerase Homo sapiens 110-113 1733231-6 1992 Recovery of pHi was also aided by HCO3(-)-dependent and DIDS-sensitive mechanisms not seen in the resting cell. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 56-60 glucose-6-phosphate isomerase Homo sapiens 12-15 1733231-8 1992 This suggests that the DIDS-sensitive pathways are activated under conditions where pHi has been shifted away from resting levels in either direction and act primarily to restore resting pHi. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 23-27 glucose-6-phosphate isomerase Homo sapiens 84-87 1733231-8 1992 This suggests that the DIDS-sensitive pathways are activated under conditions where pHi has been shifted away from resting levels in either direction and act primarily to restore resting pHi. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 23-27 glucose-6-phosphate isomerase Homo sapiens 187-190 1728060-9 1992 When a mixture of 1.0 micrograms/ml nigericin, 0.5 mM amiloride, and 0.1 mM DIDS was present in the medium, the pHi rapidly decreased by about 0.3 and 0.4 pH units at pHe 7.2 and 6.6, respectively. Nigericin 36-45 glucose-6-phosphate isomerase Homo sapiens 112-115 1728060-2 1992 Hyperthermia alone at 43 degrees C for 2 h decreased pHi of SCK cells by 0.15-0.20 pH units, as measured fluorometrically using the pH-sensitive dye BCECF. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 149-154 glucose-6-phosphate isomerase Homo sapiens 53-56 1347260-4 1992 After 14 days of metoprolol treatment (100 mg d-1) the urinary excretion of PGI-M did not differ from control (253 +/- 77 vs. 220 +/- 33 pg mg-1 creatinine, respectively). Metoprolol 17-27 glucose-6-phosphate isomerase Homo sapiens 76-79 1317479-6 1992 The most common method for pHi perturbation for the measurement of buffering is used, the rapid diffusion of ammonia across the cell membrane. Ammonia 109-116 glucose-6-phosphate isomerase Homo sapiens 27-30 1728060-3 1992 When the cells were treated with 0.5 mM amiloride at 37 degrees C, the pHi declined by 0.10-0.15 pH units at an extracellular pH (pHe) of both 7.2 and 6.6. Amiloride 40-49 glucose-6-phosphate isomerase Homo sapiens 71-74 1728060-9 1992 When a mixture of 1.0 micrograms/ml nigericin, 0.5 mM amiloride, and 0.1 mM DIDS was present in the medium, the pHi rapidly decreased by about 0.3 and 0.4 pH units at pHe 7.2 and 6.6, respectively. Amiloride 54-63 glucose-6-phosphate isomerase Homo sapiens 112-115 1728060-3 1992 When the cells were treated with 0.5 mM amiloride at 37 degrees C, the pHi declined by 0.10-0.15 pH units at an extracellular pH (pHe) of both 7.2 and 6.6. Phenylalanine 130-133 glucose-6-phosphate isomerase Homo sapiens 71-74 1728060-9 1992 When a mixture of 1.0 micrograms/ml nigericin, 0.5 mM amiloride, and 0.1 mM DIDS was present in the medium, the pHi rapidly decreased by about 0.3 and 0.4 pH units at pHe 7.2 and 6.6, respectively. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 76-80 glucose-6-phosphate isomerase Homo sapiens 112-115 1728060-6 1992 DIDS, however, enhanced the effects of amiloride in decreasing pHi and in increasing the thermoresponse of SCK cells, particularly at pHe 6.6. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 0-4 glucose-6-phosphate isomerase Homo sapiens 63-66 1728060-6 1992 DIDS, however, enhanced the effects of amiloride in decreasing pHi and in increasing the thermoresponse of SCK cells, particularly at pHe 6.6. Amiloride 39-48 glucose-6-phosphate isomerase Homo sapiens 63-66 1728060-9 1992 When a mixture of 1.0 micrograms/ml nigericin, 0.5 mM amiloride, and 0.1 mM DIDS was present in the medium, the pHi rapidly decreased by about 0.3 and 0.4 pH units at pHe 7.2 and 6.6, respectively. Phenylalanine 167-170 glucose-6-phosphate isomerase Homo sapiens 112-115 1728060-7 1992 Treatment of the cells with nigericin at 0.1-1.0 micrograms/ml lowered the pHi and enhanced the thermosensitivity of the cells in a dose-dependent manner. Nigericin 28-37 glucose-6-phosphate isomerase Homo sapiens 75-78 1659231-3 1991 The specific kappa-agonist trans-dl-3,4-dichloro-N-methyl-N-[2-(1-pyrrolidinyl)cyclohexyl]- benzene-acetamide (U-50488H) (methane sulfonate salt) caused a transient increase in cytosolic pH (pHi) measured from the change in SNARF-1 fluorescence and an increase in cytosolic [Ca2+] (Cai), indexed by a change in indo-1 fluorescence. trans-dl-3,4-dichloro-n-methyl-n-[2-(1-pyrrolidinyl)cyclohexyl]- benzene-acetamide 27-109 glucose-6-phosphate isomerase Homo sapiens 187-189 1662908-19 1991 Recovery of intracellular pH (pHi) was found to be strictly Na(+)-dependent and inhibited greater than or equal to 95% by 1 mM amiloride. Amiloride 127-136 glucose-6-phosphate isomerase Homo sapiens 30-33 1662908-23 1991 Intracellular ATP depletion, using KCN or replacement of glucose by a nonmetabolizable glucose analogue, reduced pHi recovery by 70-75% relative to control values. Adenosine Triphosphate 14-17 glucose-6-phosphate isomerase Homo sapiens 113-116 1662908-23 1991 Intracellular ATP depletion, using KCN or replacement of glucose by a nonmetabolizable glucose analogue, reduced pHi recovery by 70-75% relative to control values. Potassium Cyanide 35-38 glucose-6-phosphate isomerase Homo sapiens 113-116 1662908-23 1991 Intracellular ATP depletion, using KCN or replacement of glucose by a nonmetabolizable glucose analogue, reduced pHi recovery by 70-75% relative to control values. Glucose 57-64 glucose-6-phosphate isomerase Homo sapiens 113-116 1662908-23 1991 Intracellular ATP depletion, using KCN or replacement of glucose by a nonmetabolizable glucose analogue, reduced pHi recovery by 70-75% relative to control values. Glucose 87-94 glucose-6-phosphate isomerase Homo sapiens 113-116 1662908-26 1991 We interpret this mechanism to be an ATP-sensitive Na(+)-H+ antiporter that acts to reestablish pHi in type II alveolar epithelial cells. Adenosine Triphosphate 37-40 glucose-6-phosphate isomerase Homo sapiens 96-99 1659231-3 1991 The specific kappa-agonist trans-dl-3,4-dichloro-N-methyl-N-[2-(1-pyrrolidinyl)cyclohexyl]- benzene-acetamide (U-50488H) (methane sulfonate salt) caused a transient increase in cytosolic pH (pHi) measured from the change in SNARF-1 fluorescence and an increase in cytosolic [Ca2+] (Cai), indexed by a change in indo-1 fluorescence. trans-dl-3,4-dichloro-n-methyl-n-[2-(1-pyrrolidinyl)cyclohexyl]- benzene-acetamide 27-109 glucose-6-phosphate isomerase Homo sapiens 191-194 1659231-5 1991 Both pHi and Cai effects were blocked by the specific antagonist kappa-opioid receptor l-(N-furylmethyl)-alpha-normetazocine methane-sulfonate (Mr 1452). l-(n-furylmethyl)-alpha-normetazocine methane-sulfonate 87-142 glucose-6-phosphate isomerase Homo sapiens 5-8 1659231-8 1991 The pHi increase was abolished by 1) blockade of the Na(+)-H+ exchanger by ethyl isopropyl amiloride and 2) inhibition of protein kinase C (PKC) activity via pretreatment with staurosporine or prolonged incubation with 4 beta-phorbol 12-myristate 13-acetate. ethylisopropylamiloride 75-100 glucose-6-phosphate isomerase Homo sapiens 4-7 1659231-8 1991 The pHi increase was abolished by 1) blockade of the Na(+)-H+ exchanger by ethyl isopropyl amiloride and 2) inhibition of protein kinase C (PKC) activity via pretreatment with staurosporine or prolonged incubation with 4 beta-phorbol 12-myristate 13-acetate. Staurosporine 176-189 glucose-6-phosphate isomerase Homo sapiens 4-7 1659231-8 1991 The pHi increase was abolished by 1) blockade of the Na(+)-H+ exchanger by ethyl isopropyl amiloride and 2) inhibition of protein kinase C (PKC) activity via pretreatment with staurosporine or prolonged incubation with 4 beta-phorbol 12-myristate 13-acetate. Tetradecanoylphorbol Acetate 221-257 glucose-6-phosphate isomerase Homo sapiens 4-7 1667273-1 1991 Microspectrofluorometry was used to study the regulation of intracellular pH (pHi) in 2"-7"-bis (carboxyethyl-)-5,6-carboxyfluorescein (BCECF)-loaded astrocytes. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 86-134 glucose-6-phosphate isomerase Homo sapiens 78-81 1726709-1 1991 Interleukin-8 (IL-8) stimulated an increase in cytoplasmic-free Ca2+ ([Ca2+]i) and intracellular pH (pHi) in parallel at low concentrations (0.5 to 5 ng/mL), and stimulated O2- release and membrane depolarization in parallel at high concentrations (50 to 5,000 ng/mL). Oxygen 173-175 glucose-6-phosphate isomerase Homo sapiens 101-104 1667273-1 1991 Microspectrofluorometry was used to study the regulation of intracellular pH (pHi) in 2"-7"-bis (carboxyethyl-)-5,6-carboxyfluorescein (BCECF)-loaded astrocytes. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 136-141 glucose-6-phosphate isomerase Homo sapiens 78-81 1667273-4 1991 Furthermore, when cells were exposed to HEPES buffer with reduced or increased pHe, pHi changed in parallel. HEPES 40-45 glucose-6-phosphate isomerase Homo sapiens 84-87 1667273-4 1991 Furthermore, when cells were exposed to HEPES buffer with reduced or increased pHe, pHi changed in parallel. Phenylalanine 79-82 glucose-6-phosphate isomerase Homo sapiens 84-87 1887923-3 1991 In the absence of ATP and Mg2+, the channels had a linear current-voltage relationship during hyperpolarizing pulses (20-100 mV negative to the reversal potential) at both intracellular pH (pHi) 7.4 and 6.5, but the slope conductance was 66 +/- 2 pS at pHi 7.4 and 46 +/- 2 pS at pHi 6.5. magnesium ion 26-30 glucose-6-phosphate isomerase Homo sapiens 190-193 1951675-3 1991 Under relatively alkaline conditions [intracellular pH (pHi) greater than or equal to 7.10 and pHo greater than or equal to 7.40], the cell"s natural Cl(-)HCO3- exchanger mimicked the actions of the tributyltin compound and was the principal factor controlling steady-state pHi. Bicarbonates 155-159 glucose-6-phosphate isomerase Homo sapiens 56-59 1951675-5 1991 Exposure of neutrophils to a number of inhibitors of Cl(-)-HCO3- exchange led to a fall in pHi, apparently confirming the impression that a net HCO3- influx through Cl(-)-HCO3- countertransport was chiefly responsible for maintaining steady-state pHi. Bicarbonates 59-63 glucose-6-phosphate isomerase Homo sapiens 91-94 1951675-5 1991 Exposure of neutrophils to a number of inhibitors of Cl(-)-HCO3- exchange led to a fall in pHi, apparently confirming the impression that a net HCO3- influx through Cl(-)-HCO3- countertransport was chiefly responsible for maintaining steady-state pHi. Bicarbonates 144-148 glucose-6-phosphate isomerase Homo sapiens 247-250 1951675-5 1991 Exposure of neutrophils to a number of inhibitors of Cl(-)-HCO3- exchange led to a fall in pHi, apparently confirming the impression that a net HCO3- influx through Cl(-)-HCO3- countertransport was chiefly responsible for maintaining steady-state pHi. Bicarbonates 144-148 glucose-6-phosphate isomerase Homo sapiens 247-250 1951675-7 1991 These results imply that Cl(-)-HCO3- exchange is the dominant pH regulatory device only under relatively alkaline conditions and that other mechanisms in addition to Na(+)-H+ exchange are likely to play an important role in recovery from acidification and in maintaining steady-state pHi. Bicarbonates 31-35 glucose-6-phosphate isomerase Homo sapiens 284-287 1655031-6 1991 It is concluded that: (a) phosphorylation of the Na+/H+ exchanger is the common origin of the diverse effects of PMA, DHG, okadaic acid and staurosporine, (b) Na+/H+ exchange properties are tightly regulated by phosphorylation and dephosphorylation, and (c) the exchanger plays a major role in pHi regulation in platelets. Staurosporine 140-153 glucose-6-phosphate isomerase Homo sapiens 294-297 1887923-3 1991 In the absence of ATP and Mg2+, the channels had a linear current-voltage relationship during hyperpolarizing pulses (20-100 mV negative to the reversal potential) at both intracellular pH (pHi) 7.4 and 6.5, but the slope conductance was 66 +/- 2 pS at pHi 7.4 and 46 +/- 2 pS at pHi 6.5. magnesium ion 26-30 glucose-6-phosphate isomerase Homo sapiens 253-256 1887923-3 1991 In the absence of ATP and Mg2+, the channels had a linear current-voltage relationship during hyperpolarizing pulses (20-100 mV negative to the reversal potential) at both intracellular pH (pHi) 7.4 and 6.5, but the slope conductance was 66 +/- 2 pS at pHi 7.4 and 46 +/- 2 pS at pHi 6.5. magnesium ion 26-30 glucose-6-phosphate isomerase Homo sapiens 253-256 1887923-5 1991 However, in the presence of both 0.2 mM ATP and 1 mM MgCl2, lowering pHi from 7.4 to 6.5 increased the mean open time (from 5.0 +/- 2.6 to 17.9 +/- 5.9 ms, P less than 0.01) and the open-state probability (from 0.025 +/- 0.010 to 0.098 +/- 0.024, P less than 0.01). Adenosine Triphosphate 40-43 glucose-6-phosphate isomerase Homo sapiens 69-72 1887923-5 1991 However, in the presence of both 0.2 mM ATP and 1 mM MgCl2, lowering pHi from 7.4 to 6.5 increased the mean open time (from 5.0 +/- 2.6 to 17.9 +/- 5.9 ms, P less than 0.01) and the open-state probability (from 0.025 +/- 0.010 to 0.098 +/- 0.024, P less than 0.01). Magnesium Chloride 53-58 glucose-6-phosphate isomerase Homo sapiens 69-72 1742343-2 1991 Steady-state pHi was 7.2 in both resting (50 microM cimetidine) and stimulated (100 microM histamine) PCs. Cimetidine 52-62 glucose-6-phosphate isomerase Homo sapiens 13-16 1800793-1 1991 Extracellular ATP (adenosine 5"-triphosphate) produces a dose-dependent elevation of DNA synthesis concomitantly with raised intracellular pH (pHi) in quiescent monolayer Chang liver cells. Adenosine Triphosphate 14-17 glucose-6-phosphate isomerase Homo sapiens 143-146 1742343-2 1991 Steady-state pHi was 7.2 in both resting (50 microM cimetidine) and stimulated (100 microM histamine) PCs. Histamine 91-100 glucose-6-phosphate isomerase Homo sapiens 13-16 1800793-1 1991 Extracellular ATP (adenosine 5"-triphosphate) produces a dose-dependent elevation of DNA synthesis concomitantly with raised intracellular pH (pHi) in quiescent monolayer Chang liver cells. Adenosine Triphosphate 19-44 glucose-6-phosphate isomerase Homo sapiens 143-146 1742343-5 1991 Maximum velocity (Vmax) for Cl influx or HCO3 efflux was 80-110 mM/min at pHi 7.6-7.8, and the Km for extracellular concentrations of Cl (Clo) was 25 mM; in the physiological range (pHi 7.1-7.3), Vmax for anion fluxes was approximately 50 mM/min. Bicarbonates 41-45 glucose-6-phosphate isomerase Homo sapiens 74-77 1742343-5 1991 Maximum velocity (Vmax) for Cl influx or HCO3 efflux was 80-110 mM/min at pHi 7.6-7.8, and the Km for extracellular concentrations of Cl (Clo) was 25 mM; in the physiological range (pHi 7.1-7.3), Vmax for anion fluxes was approximately 50 mM/min. clo 138-141 glucose-6-phosphate isomerase Homo sapiens 74-77 1742343-5 1991 Maximum velocity (Vmax) for Cl influx or HCO3 efflux was 80-110 mM/min at pHi 7.6-7.8, and the Km for extracellular concentrations of Cl (Clo) was 25 mM; in the physiological range (pHi 7.1-7.3), Vmax for anion fluxes was approximately 50 mM/min. clo 138-141 glucose-6-phosphate isomerase Homo sapiens 182-185 1664334-2 1991 Using the fluorescent dyes 2,7-bis(carboxyethyl)carboxyfluorescein acetoxymethyl ester (BCECF) and fura-2, the resting pHi and [Ca2+]i in suspended cells were 7.23 +/- 0.03 and 209 +/- 30 nM; those in adherent cells were 7.28 +/- 0.02 and 87 +/- 5 nM. 2,7-bis(carboxyethyl)carboxyfluorescein acetoxymethyl ester 27-86 glucose-6-phosphate isomerase Homo sapiens 119-122 1664334-13 1991 1,2-bis(o-Aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA) (100 microM), a Ca2+ chelator, induced a decrease in pHi as well as a reduction of [Ca2+]i, also supporting the direct relation between pHi and [Ca2+]i. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 58-63 glucose-6-phosphate isomerase Homo sapiens 118-121 1651331-3 1991 This pHi increase was Na(+)-dependent and was inhibited by 5,N-disubstituted analogs of amiloride, indicating mediation by the Na+/H+ antiport. 5-doxylstearic acid 59-62 glucose-6-phosphate isomerase Homo sapiens 5-8 1651331-3 1991 This pHi increase was Na(+)-dependent and was inhibited by 5,N-disubstituted analogs of amiloride, indicating mediation by the Na+/H+ antiport. Amiloride 88-97 glucose-6-phosphate isomerase Homo sapiens 5-8 1664334-13 1991 1,2-bis(o-Aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA) (100 microM), a Ca2+ chelator, induced a decrease in pHi as well as a reduction of [Ca2+]i, also supporting the direct relation between pHi and [Ca2+]i. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 58-63 glucose-6-phosphate isomerase Homo sapiens 201-204 1664334-16 1991 Resting pHi and [Ca2+]i in cells treated with either 8-bromo-dibutyryl cAMP (1 mM) or forskolin (150 microM) are not changed. 8-bromo-dibutyryl camp 53-75 glucose-6-phosphate isomerase Homo sapiens 8-11 1664334-7 1991 The alteration in [Ca2+]i induced by modification of pHi was abolished in the absence of external Ca2+ or by adding 2 mM CoCl2, LaCl3, and attenuated by the addition of 2 mM MnCl2 to the bathing medium. cobaltous chloride 121-126 glucose-6-phosphate isomerase Homo sapiens 53-56 1664334-7 1991 The alteration in [Ca2+]i induced by modification of pHi was abolished in the absence of external Ca2+ or by adding 2 mM CoCl2, LaCl3, and attenuated by the addition of 2 mM MnCl2 to the bathing medium. lanthanum chloride 128-133 glucose-6-phosphate isomerase Homo sapiens 53-56 1664334-7 1991 The alteration in [Ca2+]i induced by modification of pHi was abolished in the absence of external Ca2+ or by adding 2 mM CoCl2, LaCl3, and attenuated by the addition of 2 mM MnCl2 to the bathing medium. manganese chloride 174-179 glucose-6-phosphate isomerase Homo sapiens 53-56 1664334-9 1991 CoCl2, LaCl3, and MnCl2 each induced changes in pHi and [Ca2+]i but verapamil and nifedipine did not. cobaltous chloride 0-5 glucose-6-phosphate isomerase Homo sapiens 48-51 1664334-9 1991 CoCl2, LaCl3, and MnCl2 each induced changes in pHi and [Ca2+]i but verapamil and nifedipine did not. lanthanum chloride 7-12 glucose-6-phosphate isomerase Homo sapiens 48-51 1664334-9 1991 CoCl2, LaCl3, and MnCl2 each induced changes in pHi and [Ca2+]i but verapamil and nifedipine did not. manganese chloride 18-23 glucose-6-phosphate isomerase Homo sapiens 48-51 1664334-13 1991 1,2-bis(o-Aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA) (100 microM), a Ca2+ chelator, induced a decrease in pHi as well as a reduction of [Ca2+]i, also supporting the direct relation between pHi and [Ca2+]i. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 0-56 glucose-6-phosphate isomerase Homo sapiens 118-121 1664334-13 1991 1,2-bis(o-Aminophenoxy)ethane-N,N,N",N"-tetraacetic acid (BAPTA) (100 microM), a Ca2+ chelator, induced a decrease in pHi as well as a reduction of [Ca2+]i, also supporting the direct relation between pHi and [Ca2+]i. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 0-56 glucose-6-phosphate isomerase Homo sapiens 201-204 1898035-1 1991 The effect of matrix pH (pHi) on the activity of the mitochondrial K+/H+ antiport has been studied using the fluorescence of 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) to monitor pHi and passive swelling in K+ acetate to follow antiport activity. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 125-168 glucose-6-phosphate isomerase Homo sapiens 25-28 1898035-1 1991 The effect of matrix pH (pHi) on the activity of the mitochondrial K+/H+ antiport has been studied using the fluorescence of 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) to monitor pHi and passive swelling in K+ acetate to follow antiport activity. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 170-175 glucose-6-phosphate isomerase Homo sapiens 25-28 1898035-3 1991 Addition of A23187 to deplete matrix Mg2+ results in a further acid shift in pHi followed by equilibration of delta pH. Calcimycin 12-18 glucose-6-phosphate isomerase Homo sapiens 77-80 1898035-3 1991 Addition of A23187 to deplete matrix Mg2+ results in a further acid shift in pHi followed by equilibration of delta pH. magnesium ion 37-41 glucose-6-phosphate isomerase Homo sapiens 77-80 1869926-4 1991 pHi was measured using fluorescent pH probes and was manipulated by changing extracellular pH (pHe). Phenylalanine 95-98 glucose-6-phosphate isomerase Homo sapiens 0-3 1869926-6 1991 Neuronal and glial death was a function of both the degree and the duration of intracellular acidification, such that the LD50 following timed exposure to HCl increased from pH, 3.5 for 10-min acid incubations to pHi 5.9 for 2-hr exposures and pHi 6.5 for 6-hr exposures. Hydrochloric Acid 155-158 glucose-6-phosphate isomerase Homo sapiens 213-216 1869926-6 1991 Neuronal and glial death was a function of both the degree and the duration of intracellular acidification, such that the LD50 following timed exposure to HCl increased from pH, 3.5 for 10-min acid incubations to pHi 5.9 for 2-hr exposures and pHi 6.5 for 6-hr exposures. Hydrochloric Acid 155-158 glucose-6-phosphate isomerase Homo sapiens 244-247 1869926-7 1991 Replacement of HCl with lactic acid raised the LD50 to pHi 4.5 for 10-min acid exposures, but did not change the LD50 for longer exposures: pHi measurements concurrent with extracellular acidification suggested that the greater cytotoxicity of lactic acid relative to that of HCl was caused by the more rapid intracellular acidification associated with lactic acid. Hydrochloric Acid 15-18 glucose-6-phosphate isomerase Homo sapiens 55-58 1869926-7 1991 Replacement of HCl with lactic acid raised the LD50 to pHi 4.5 for 10-min acid exposures, but did not change the LD50 for longer exposures: pHi measurements concurrent with extracellular acidification suggested that the greater cytotoxicity of lactic acid relative to that of HCl was caused by the more rapid intracellular acidification associated with lactic acid. Hydrochloric Acid 15-18 glucose-6-phosphate isomerase Homo sapiens 140-143 1869926-7 1991 Replacement of HCl with lactic acid raised the LD50 to pHi 4.5 for 10-min acid exposures, but did not change the LD50 for longer exposures: pHi measurements concurrent with extracellular acidification suggested that the greater cytotoxicity of lactic acid relative to that of HCl was caused by the more rapid intracellular acidification associated with lactic acid. Lactic Acid 24-35 glucose-6-phosphate isomerase Homo sapiens 55-58 1869926-7 1991 Replacement of HCl with lactic acid raised the LD50 to pHi 4.5 for 10-min acid exposures, but did not change the LD50 for longer exposures: pHi measurements concurrent with extracellular acidification suggested that the greater cytotoxicity of lactic acid relative to that of HCl was caused by the more rapid intracellular acidification associated with lactic acid. Lactic Acid 24-35 glucose-6-phosphate isomerase Homo sapiens 140-143 1649192-2 1991 Under nonreducing conditions, AMF migrates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band of 55 kDa but under reducing conditions as a band of 64 kDa. Sodium Dodecyl Sulfate 46-68 glucose-6-phosphate isomerase Homo sapiens 30-33 1649184-0 1991 Superoxide anion increases intracellular pH, intracellular free calcium, and arachidonate release in human amnion cells. Superoxides 0-16 glucose-6-phosphate isomerase Homo sapiens 41-43 1649192-2 1991 Under nonreducing conditions, AMF migrates in sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band of 55 kDa but under reducing conditions as a band of 64 kDa. polyacrylamide 69-83 glucose-6-phosphate isomerase Homo sapiens 30-33 1649184-1 1991 We determined the effects of superoxide anion, produced by addition of xanthine oxidase to hypoxanthine, on the intracellular pH (pHi) and intracellular free calcium concentration ([Ca2+]i) and release of arachidonate in human cultured amnion cells. Superoxides 29-45 glucose-6-phosphate isomerase Homo sapiens 126-128 1649192-3 1991 Two-dimensional polyacrylamide gel electrophoresis of the purified AMF resolved two groups of polypeptides with isoelectric points of 6.1 and 6.2 (majors), 6.35 and 6.4 (minors). polyacrylamide 16-30 glucose-6-phosphate isomerase Homo sapiens 67-70 1649184-1 1991 We determined the effects of superoxide anion, produced by addition of xanthine oxidase to hypoxanthine, on the intracellular pH (pHi) and intracellular free calcium concentration ([Ca2+]i) and release of arachidonate in human cultured amnion cells. Superoxides 29-45 glucose-6-phosphate isomerase Homo sapiens 130-133 1647205-0 1991 Regulation of the phosphoinositide cycle by Na+/H+ exchange and intracellular pH in human platelets. Phosphatidylinositols 18-34 glucose-6-phosphate isomerase Homo sapiens 78-80 1649184-1 1991 We determined the effects of superoxide anion, produced by addition of xanthine oxidase to hypoxanthine, on the intracellular pH (pHi) and intracellular free calcium concentration ([Ca2+]i) and release of arachidonate in human cultured amnion cells. Hypoxanthine 91-103 glucose-6-phosphate isomerase Homo sapiens 126-128 1649184-1 1991 We determined the effects of superoxide anion, produced by addition of xanthine oxidase to hypoxanthine, on the intracellular pH (pHi) and intracellular free calcium concentration ([Ca2+]i) and release of arachidonate in human cultured amnion cells. Hypoxanthine 91-103 glucose-6-phosphate isomerase Homo sapiens 130-133 1649184-2 1991 Superoxide anion induced a prompt increase of pHi and subsequent increase of [Ca2+]i. Superoxides 0-16 glucose-6-phosphate isomerase Homo sapiens 46-49 1649184-5 1991 NH4Cl, which can generally increase pHi, also increased [Ca2+]i of amnion cells. Ammonium Chloride 0-5 glucose-6-phosphate isomerase Homo sapiens 36-39 1649184-6 1991 But the increase of [Ca2+]i induced by the NH4Cl was significantly less than that induced by the amount of superoxide anion causing a similar increase in pHi. Ammonium Chloride 43-48 glucose-6-phosphate isomerase Homo sapiens 154-157 1649184-6 1991 But the increase of [Ca2+]i induced by the NH4Cl was significantly less than that induced by the amount of superoxide anion causing a similar increase in pHi. Superoxides 107-123 glucose-6-phosphate isomerase Homo sapiens 154-157 1649184-7 1991 These results show that superoxide anion, crossed through anion channel in membrane, increased [Ca2+]i at least partially via increase of pHi and that the calcium mobilization was dependent on both extracellular and intracellular sources. Superoxides 24-40 glucose-6-phosphate isomerase Homo sapiens 138-141 1649184-12 1991 These findings suggested that superoxide anion may regulate biological functions in amnion cells via pHi, [Ca2+]i mobilization, and the release of arachidonate without damaging the cells. Superoxides 30-46 glucose-6-phosphate isomerase Homo sapiens 101-104 2050661-4 1991 Intracellular pH (pHi) recording in individual transfectants loaded with the fluorescent pHi indicator, 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein, was used to determine the flux of HCO3- as a measure of Cl-/HCO3- exchange activity. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 104-150 glucose-6-phosphate isomerase Homo sapiens 18-21 2050661-4 1991 Intracellular pH (pHi) recording in individual transfectants loaded with the fluorescent pHi indicator, 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein, was used to determine the flux of HCO3- as a measure of Cl-/HCO3- exchange activity. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 104-150 glucose-6-phosphate isomerase Homo sapiens 89-92 1712019-4 1991 On treatment with V8 protease, GP-I was converted to two glycopeptides, one with poor reactivity and the other with intermediate reactivity. Glycopeptides 57-70 glucose-6-phosphate isomerase Homo sapiens 31-35 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Leucine 57-60 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Serine 61-64 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Glutamic Acid 66-69 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Serine 70-73 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Threonine 75-78 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Threonine 80-83 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Glutamine 85-88 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Leucine 89-92 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Proline 93-96 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Glycine 97-100 glucose-6-phosphate isomerase Homo sapiens 0-4 1712019-6 1991 GP-II corresponded to a part of GP-I, its sequence being Leu-Ser*-Glu-Ser*-Thr*-Thr*-Gln-Leu-Pro-Gly, where asterisks denote amino acids to which an alpha-GalNAc residue is attached. Acetylgalactosamine 149-161 glucose-6-phosphate isomerase Homo sapiens 0-4 1647205-7 1991 These data suggest a model in which Na+/H+ and pHi play an important regulatory role in permitting the phosphoinositide cycle to proceed in thrombin-activated platelets. Phosphatidylinositols 103-119 glucose-6-phosphate isomerase Homo sapiens 47-50 1647205-2 1991 This EIA effect was further synergistically enhanced by lowering intracellular pH (pHi) with either nigericin or sodium propionate, and reversed by raising pHi with monensin or ammonium chloride. Nigericin 100-109 glucose-6-phosphate isomerase Homo sapiens 79-81 1647205-2 1991 This EIA effect was further synergistically enhanced by lowering intracellular pH (pHi) with either nigericin or sodium propionate, and reversed by raising pHi with monensin or ammonium chloride. Nigericin 100-109 glucose-6-phosphate isomerase Homo sapiens 83-86 1647205-2 1991 This EIA effect was further synergistically enhanced by lowering intracellular pH (pHi) with either nigericin or sodium propionate, and reversed by raising pHi with monensin or ammonium chloride. sodium propionate 113-130 glucose-6-phosphate isomerase Homo sapiens 79-81 1647205-2 1991 This EIA effect was further synergistically enhanced by lowering intracellular pH (pHi) with either nigericin or sodium propionate, and reversed by raising pHi with monensin or ammonium chloride. sodium propionate 113-130 glucose-6-phosphate isomerase Homo sapiens 83-86 1647205-2 1991 This EIA effect was further synergistically enhanced by lowering intracellular pH (pHi) with either nigericin or sodium propionate, and reversed by raising pHi with monensin or ammonium chloride. Monensin 165-173 glucose-6-phosphate isomerase Homo sapiens 156-159 1646163-7 1991 The application of norepinephrine or 125 mM K+ solution induced contraction in the arterial segments with an accompanying fall in pHi. Norepinephrine 19-33 glucose-6-phosphate isomerase Homo sapiens 130-133 1646163-2 1991 Segments of isolated resistance arteries were mounted in a myograph and loaded with the pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein 117-166 glucose-6-phosphate isomerase Homo sapiens 88-90 2058696-0 1991 Na(+)-HCO3- symport modulates intracellular pH in alveolar epithelial cells. Bicarbonates 6-10 glucose-6-phosphate isomerase Homo sapiens 44-46 2058696-4 1991 pHi recovered in the presence of Na+ with an initial rate (dpHi/dt) of 0.15 min-1, which was reduced by 67% when Na+ was replaced by choline, unaffected by substitution of gluconate for Cl-, reduced 40% in the presence of 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS, 500 microM), and unchanged by amiloride (1 mM). dphi 59-63 glucose-6-phosphate isomerase Homo sapiens 0-3 2058696-4 1991 pHi recovered in the presence of Na+ with an initial rate (dpHi/dt) of 0.15 min-1, which was reduced by 67% when Na+ was replaced by choline, unaffected by substitution of gluconate for Cl-, reduced 40% in the presence of 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS, 500 microM), and unchanged by amiloride (1 mM). Thymidine 64-66 glucose-6-phosphate isomerase Homo sapiens 0-3 2058696-4 1991 pHi recovered in the presence of Na+ with an initial rate (dpHi/dt) of 0.15 min-1, which was reduced by 67% when Na+ was replaced by choline, unaffected by substitution of gluconate for Cl-, reduced 40% in the presence of 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS, 500 microM), and unchanged by amiloride (1 mM). Choline 133-140 glucose-6-phosphate isomerase Homo sapiens 0-3 2058696-4 1991 pHi recovered in the presence of Na+ with an initial rate (dpHi/dt) of 0.15 min-1, which was reduced by 67% when Na+ was replaced by choline, unaffected by substitution of gluconate for Cl-, reduced 40% in the presence of 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS, 500 microM), and unchanged by amiloride (1 mM). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 222-270 glucose-6-phosphate isomerase Homo sapiens 0-3 2058696-4 1991 pHi recovered in the presence of Na+ with an initial rate (dpHi/dt) of 0.15 min-1, which was reduced by 67% when Na+ was replaced by choline, unaffected by substitution of gluconate for Cl-, reduced 40% in the presence of 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS, 500 microM), and unchanged by amiloride (1 mM). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 272-276 glucose-6-phosphate isomerase Homo sapiens 0-3 2058696-4 1991 pHi recovered in the presence of Na+ with an initial rate (dpHi/dt) of 0.15 min-1, which was reduced by 67% when Na+ was replaced by choline, unaffected by substitution of gluconate for Cl-, reduced 40% in the presence of 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS, 500 microM), and unchanged by amiloride (1 mM). Amiloride 308-317 glucose-6-phosphate isomerase Homo sapiens 0-3 2058696-5 1991 In parallel experiments, cells were incubated at pH 7.4 with 20 mM HCO3- and pHi acutely lowered by NH3 prepulse. Bicarbonates 67-71 glucose-6-phosphate isomerase Homo sapiens 49-51 2058696-8 1991 This ion transport mechanism may contribute to regulation of pHi in alveolar pneumocytes, transepithelial transport of acid-base equivalents across the alveolar epithelium, and modulation of pH of alveolar fluid in adult mammalian lungs. acid-base 119-128 glucose-6-phosphate isomerase Homo sapiens 61-63 1646163-6 1991 The application of ethylisopropylamiloride (EIPA) and 4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid (DIDS) demonstrated that both Na(+)-H+ exchange and bicarbonate-dependent membrane mechanisms contributed to pHi homeostasis but that neither system was overactive in hypertension (pHi change with EIPA in vessels from hypertensive versus control subjects was -0.11 +/- 0.02 and 0.13 +/- 0.03 pH units, respectively, and pHi change with DIDS in vessels from hypertensive versus control subjects was -0.097 +/- 0.05 and -0.091 +/- 0.03 pH units, respectively). 4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid 54-104 glucose-6-phosphate isomerase Homo sapiens 214-216 1645954-2 1991 Inhibition of the Na+/H(+)-antiporter by dimethylamiloride or a reduction of external Na(+)-concentrations attenuates the increases in pHi and [Ca2+]i. 5-dimethylamiloride 41-58 glucose-6-phosphate isomerase Homo sapiens 135-138 1656038-1 1991 The sodium dependence of recovery of cytoplasmic pH (pHi) after an acid load was studied in platelets from 15 patients with untreated essential hypertension (mean arterial pressure 117 +/- 2.3 mmHg, mean +/- SE) and in 15 normotensive controls (mean arterial pressure 90 +/- 2.2 mmHg). Sodium 4-10 glucose-6-phosphate isomerase Homo sapiens 53-56 1900792-5 1991 When populational platelets were preloaded with the Ca2+ chelator BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid), the thrombin-induced initial large increase in Cai was apparently inhibited, whereas the pHi decrease induced by thrombin was not altered. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 66-71 glucose-6-phosphate isomerase Homo sapiens 221-224 1900792-5 1991 When populational platelets were preloaded with the Ca2+ chelator BAPTA (1,2-bis(o-aminophenoxy)ethane-N,N,N",N"-tetraacetic acid), the thrombin-induced initial large increase in Cai was apparently inhibited, whereas the pHi decrease induced by thrombin was not altered. 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid 73-129 glucose-6-phosphate isomerase Homo sapiens 221-224 1900792-8 1991 In both single and populational cell types, application of the K(+)-H+ ionophore nigericin, which induced a transient decrease in pHi, led to the release of Ca2+ from intracellular stores. Nigericin 81-90 glucose-6-phosphate isomerase Homo sapiens 130-133 1900792-9 1991 In single parietal cells double-labeled with fura-2 and BCECF, a temporal decrease in pHi preceded the rise in Cai after stimulation with nigericin. Fura-2 45-51 glucose-6-phosphate isomerase Homo sapiens 86-89 1900792-9 1991 In single parietal cells double-labeled with fura-2 and BCECF, a temporal decrease in pHi preceded the rise in Cai after stimulation with nigericin. bcecf 56-61 glucose-6-phosphate isomerase Homo sapiens 86-89 1900792-9 1991 In single parietal cells double-labeled with fura-2 and BCECF, a temporal decrease in pHi preceded the rise in Cai after stimulation with nigericin. Nigericin 138-147 glucose-6-phosphate isomerase Homo sapiens 86-89 1900792-11 1991 In single parietal cells, replacement of medium Na+ with N-methyl-D-glucamine (NMG+), which also induced a decrease in pHi, resulted in repetitive Ca2+ spike oscillations. Meglumine 57-77 glucose-6-phosphate isomerase Homo sapiens 119-122 1900792-11 1991 In single parietal cells, replacement of medium Na+ with N-methyl-D-glucamine (NMG+), which also induced a decrease in pHi, resulted in repetitive Ca2+ spike oscillations. nmg+ 79-83 glucose-6-phosphate isomerase Homo sapiens 119-122 1677416-5 1991 Concentrations of gamma-aminobutyric acid (GABA) in the external segment (GPe) and internal segment (GPi) of GP and ST are decreased to 62, 45 and 55% of the control mean, respectively. gamma-Aminobutyric Acid 18-41 glucose-6-phosphate isomerase Homo sapiens 101-104 2012607-5 1991 The increase in pHi was dependent on extracellular Na+ and inhibited by 5"-(NN-dimethyl)amiloride, consistent with mediation by Na+/H+ exchange. 5"-(nn-dimethyl)amiloride 72-97 glucose-6-phosphate isomerase Homo sapiens 16-19 1711091-1 1991 The effect of gamma-aminobutyric acid (GABA) on intracellular pH (pHi) was examined in the crayfish stretch-receptor neurone using H(+)-selective microelectrodes and a two-microelectrode voltage clamp. gamma-Aminobutyric Acid 14-37 glucose-6-phosphate isomerase Homo sapiens 66-69 1711091-1 1991 The effect of gamma-aminobutyric acid (GABA) on intracellular pH (pHi) was examined in the crayfish stretch-receptor neurone using H(+)-selective microelectrodes and a two-microelectrode voltage clamp. gamma-Aminobutyric Acid 39-43 glucose-6-phosphate isomerase Homo sapiens 66-69 1711091-2 1991 In the presence of 30 mmol l-1 HCO3- (pH 7.4), application of GABA (0.5 mmol l-1) produced a mean fall in pHi of 0.26 units. Bicarbonates 31-35 glucose-6-phosphate isomerase Homo sapiens 106-109 1711091-2 1991 In the presence of 30 mmol l-1 HCO3- (pH 7.4), application of GABA (0.5 mmol l-1) produced a mean fall in pHi of 0.26 units. gamma-Aminobutyric Acid 62-66 glucose-6-phosphate isomerase Homo sapiens 106-109 1711091-6 1991 Acetazolamide (0.1 mmol l-1) decreased the rate of fall of pHi caused by a step increase in CO2 partial pressure as well as by GABA, which indicates that the neurone contains carbonic anhydrase. Acetazolamide 0-13 glucose-6-phosphate isomerase Homo sapiens 59-62 1711091-6 1991 Acetazolamide (0.1 mmol l-1) decreased the rate of fall of pHi caused by a step increase in CO2 partial pressure as well as by GABA, which indicates that the neurone contains carbonic anhydrase. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 92-95 glucose-6-phosphate isomerase Homo sapiens 59-62 1711091-6 1991 Acetazolamide (0.1 mmol l-1) decreased the rate of fall of pHi caused by a step increase in CO2 partial pressure as well as by GABA, which indicates that the neurone contains carbonic anhydrase. gamma-Aminobutyric Acid 127-131 glucose-6-phosphate isomerase Homo sapiens 59-62 1711091-9 1991 All these observations support the conclusion that the GABA-induced fall in pHi is due to a net efflux of HCO3- through the inhibitory anion channels. gamma-Aminobutyric Acid 55-59 glucose-6-phosphate isomerase Homo sapiens 76-79 1711091-9 1991 All these observations support the conclusion that the GABA-induced fall in pHi is due to a net efflux of HCO3- through the inhibitory anion channels. Bicarbonates 106-110 glucose-6-phosphate isomerase Homo sapiens 76-79 1848402-1 1991 We have shown that pHi in proliferating VSMC is more alkaline (7.25) than in growth-arrested cells (7.10). vsmc 40-44 glucose-6-phosphate isomerase Homo sapiens 19-22 1677416-5 1991 Concentrations of gamma-aminobutyric acid (GABA) in the external segment (GPe) and internal segment (GPi) of GP and ST are decreased to 62, 45 and 55% of the control mean, respectively. gamma-Aminobutyric Acid 43-47 glucose-6-phosphate isomerase Homo sapiens 101-104 1677416-6 1991 Concentrations of glutamic acid are increased in GPi (144%) and ST (134%). Glutamic Acid 18-31 glucose-6-phosphate isomerase Homo sapiens 49-52 1677416-8 1991 Noradrenaline (NA) concentrations in GPe and GPi are 56 and 43% of the control mean, respectively. Norepinephrine 0-13 glucose-6-phosphate isomerase Homo sapiens 45-48 1989762-1 1991 OBJECTIVE: To determine if intraluminal production of CO2 leads to underestimation of gastric intramural pH (pHi) by tonometry. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 54-57 glucose-6-phosphate isomerase Homo sapiens 109-112 1713493-3 1991 Five minutes after the addition of EIPA 60 microM, an inhibitor of Na+/H+ exchange, the pHi fell in 0.075 +/- 0.022 pH units (P less than 0.05). ethylisopropylamiloride 35-39 glucose-6-phosphate isomerase Homo sapiens 88-91 1846315-8 1991 High concentrations of amiloride, 1.0 mM for 1 h in combination with low Na+ concentrations, caused a strong pHi decrease in glioma cells but only a slight decrease in the colon carcinoma cells. Amiloride 23-32 glucose-6-phosphate isomerase Homo sapiens 109-112 1989762-7 1991 MEASUREMENTS AND MAIN RESULTS: Gastric pHi was calculated from the arterial (HCO3-) and the tonometrically determined intraluminal PCO2 using the Henderson-Hasselbalch equation. Bicarbonates 77-81 glucose-6-phosphate isomerase Homo sapiens 39-42 1989762-7 1991 MEASUREMENTS AND MAIN RESULTS: Gastric pHi was calculated from the arterial (HCO3-) and the tonometrically determined intraluminal PCO2 using the Henderson-Hasselbalch equation. pco2 131-135 glucose-6-phosphate isomerase Homo sapiens 39-42 1989762-10 1991 Mean calculated gastric pHi was 7.30 +/- 0.11 in the untreated group and 7.39 +/- 0.03 in the ranitidine-treated group (p less than .03). Ranitidine 94-104 glucose-6-phosphate isomerase Homo sapiens 24-27 1989762-11 1991 CONCLUSIONS: These data suggest that intraluminal production of CO2 from the titration of gastric HCO3- by secreted H+ can result in the underestimation of gastric pHi by tonometry. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 64-67 glucose-6-phosphate isomerase Homo sapiens 164-167 1989762-11 1991 CONCLUSIONS: These data suggest that intraluminal production of CO2 from the titration of gastric HCO3- by secreted H+ can result in the underestimation of gastric pHi by tonometry. Bicarbonates 98-102 glucose-6-phosphate isomerase Homo sapiens 164-167 2029454-1 1991 31P NMR spectroscopy provides a means of monitoring intracellular pH (pHi). ET bromodomain inhibitor 0-3 glucose-6-phosphate isomerase Homo sapiens 66-68 1991688-2 1991 Each of the three substances caused a decrease of intracellular pH (pHi) when the cell lines were exposed at low extracellular pH (pHe) in the range 6.0-6.5. Phenylalanine 131-134 glucose-6-phosphate isomerase Homo sapiens 68-71 1991688-4 1991 The fall in pHi increased with increasing dose of each agent and with decreasing pHe. Phenylalanine 81-84 glucose-6-phosphate isomerase Homo sapiens 12-15 1991688-5 1991 The pHi recovered to almost normal values after exposure of 30 minutes to 50 mM lactate, but there was little or no recovery of pHi in the presence of succinate or monomethylsuccinate. Lactic Acid 80-87 glucose-6-phosphate isomerase Homo sapiens 4-7 2029454-1 1991 31P NMR spectroscopy provides a means of monitoring intracellular pH (pHi). ET bromodomain inhibitor 0-3 glucose-6-phosphate isomerase Homo sapiens 70-73 1847303-2 1991 From the 31P-NMR spectrum of a suspension of TALH-SVE cells using the chemical shift of the intracellular inorganic phosphate a value of 7.24 +/- 0.04 for the steady-state intracellular pH (pHi) was determined at pHo = 7.40. ET bromodomain inhibitor 9-12 glucose-6-phosphate isomerase Homo sapiens 190-193 1847303-2 1991 From the 31P-NMR spectrum of a suspension of TALH-SVE cells using the chemical shift of the intracellular inorganic phosphate a value of 7.24 +/- 0.04 for the steady-state intracellular pH (pHi) was determined at pHo = 7.40. Phosphates 106-125 glucose-6-phosphate isomerase Homo sapiens 190-193 1655517-4 1991 Signal transduction following binding of AMF to its receptor, a cell surface glycoprotein of 78 kD (gp78), is mediated by a pertussis toxin sensitive G protein, inositol phosphate production and the phosphorylation of gp78. Inositol Phosphates 161-179 glucose-6-phosphate isomerase Homo sapiens 41-44 1843837-2 1991 31P and 1H spectroscopy are particularly suitable for monitoring the cerebral energy metabolism by determining the cerebral levels of ATP, ADP, phosphocreatine (PCr), inorganic phosphate (Pi), lactate and intracellular pH (pHi). ET bromodomain inhibitor 0-3 glucose-6-phosphate isomerase Homo sapiens 223-226 1987775-3 1991 Both the Cl- efflux and intracellular pH (pHi) transient are dependent on extracellular HCO3- and are sensitive to inhibition by SITS and alpha-cyano-4-hydroxycinnamate, which block anion exchange, thereby indicating that these processes are due to the countertransport of internal Cl- for external HCO3-. Bicarbonates 88-92 glucose-6-phosphate isomerase Homo sapiens 42-45 1987775-3 1991 Both the Cl- efflux and intracellular pH (pHi) transient are dependent on extracellular HCO3- and are sensitive to inhibition by SITS and alpha-cyano-4-hydroxycinnamate, which block anion exchange, thereby indicating that these processes are due to the countertransport of internal Cl- for external HCO3-. 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 129-133 glucose-6-phosphate isomerase Homo sapiens 42-45 1987775-3 1991 Both the Cl- efflux and intracellular pH (pHi) transient are dependent on extracellular HCO3- and are sensitive to inhibition by SITS and alpha-cyano-4-hydroxycinnamate, which block anion exchange, thereby indicating that these processes are due to the countertransport of internal Cl- for external HCO3-. alpha-cyano-4-hydroxycinnamate 138-168 glucose-6-phosphate isomerase Homo sapiens 42-45 1987775-3 1991 Both the Cl- efflux and intracellular pH (pHi) transient are dependent on extracellular HCO3- and are sensitive to inhibition by SITS and alpha-cyano-4-hydroxycinnamate, which block anion exchange, thereby indicating that these processes are due to the countertransport of internal Cl- for external HCO3-. Bicarbonates 299-303 glucose-6-phosphate isomerase Homo sapiens 42-45 1987775-5 1991 The relationship to pHi follows a Hill equation with pK" approximately 7.40 and Hill coefficient of 3.3, thereby suggesting that approximately 3 HCO3- may be required to bind to the modifier site. Bicarbonates 145-149 glucose-6-phosphate isomerase Homo sapiens 20-23 1987776-4 1991 Cytoplasmic pH (pHi) was monitored in cells loaded with the fluorescent, intracellularly trapped pH-sensitive probe 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 116-162 glucose-6-phosphate isomerase Homo sapiens 16-19 1987776-9 1991 Vasoconstrictor arachidonate metabolites may control glomerular cell function by a signaling mechanism centered on concurrent changes of pHi and [Ca2+]i. Arachidonic Acid 16-28 glucose-6-phosphate isomerase Homo sapiens 137-140 1834563-7 1991 At a bath pH of 7.5, the mean initial intracellular pH (pHi) was 7.32 (SD 0.03, n = 6) in HEPES-buffered Ringer"s solution and 7.39 (SD 0.1, n = 6) in HCO3-/CO2 buffered solution. HEPES 90-95 glucose-6-phosphate isomerase Homo sapiens 56-59 1834563-9 1991 Superfusion and withdrawal of 15 mmol/l NH4+ induced an acidification of 0.2 to 0.3 pH units, which recovered toward the original steady-state pHi. Ammonium Compounds 40-44 glucose-6-phosphate isomerase Homo sapiens 143-146 1660575-3 1991 Measurements were made using the pH-sensitive fluorescent dye bis (carboxyethyl) carboxyfluorescein (BCECF). bcecf 101-106 glucose-6-phosphate isomerase Homo sapiens 33-35 15946025-2 1991 Unlike integral membrane proteins, which traverse the membrane with one or more hydrophobic peptide domains, the peptide domains of these more newly described proteins are entirely extracellular and are anchored to the cell membrane via a phosphatidylinositol-glycan (GPI) anchor. Glycosylphosphatidylinositols 239-266 glucose-6-phosphate isomerase Homo sapiens 268-271 1660575-3 1991 Measurements were made using the pH-sensitive fluorescent dye bis (carboxyethyl) carboxyfluorescein (BCECF). bis (carboxyethyl) carboxyfluorescein 62-99 glucose-6-phosphate isomerase Homo sapiens 33-35 2145772-0 1990 ATP-dependent pHi recovery in lung macrophages: evidence for a plasma membrane H(+)-ATPase. Adenosine Triphosphate 0-3 glucose-6-phosphate isomerase Homo sapiens 14-17 2241164-1 1990 Previously undetected isoforms of human glucose-6-phosphate isomerase (GPI) have been isolated utilizing substrate-induced elution of the enzyme from spherical cross-linked phosphocellulose as an affinity ligand and subjected to a series of physical and chemical studies. phosphocellulose 173-189 glucose-6-phosphate isomerase Homo sapiens 40-69 2241164-1 1990 Previously undetected isoforms of human glucose-6-phosphate isomerase (GPI) have been isolated utilizing substrate-induced elution of the enzyme from spherical cross-linked phosphocellulose as an affinity ligand and subjected to a series of physical and chemical studies. phosphocellulose 173-189 glucose-6-phosphate isomerase Homo sapiens 71-74 2171349-1 1990 Regulation of intracellular pH (pHi) via a Na(+)-H+ exchange-dependent mechanism was studied in cultured human umbilical vein endothelial cells (HEC) using the pH-sensitive fluorescent dye 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein, as well as measuring 22Na influx. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 189-235 glucose-6-phosphate isomerase Homo sapiens 32-35 2171349-2 1990 Basal pHi of HEC incubated in a bicarbonate-free Na+ medium was 6.99 +/- 0.03. Bicarbonates 32-43 glucose-6-phosphate isomerase Homo sapiens 6-9 2171349-3 1990 In HEC that had been acid-loaded using nigericin or a NH4Cl prepulse, pHi recovery occurred via a Na(+)-dependent mechanism that was inhibited by 5-(N-ethyl-N-isopropyl)amiloride (EIPA). Nigericin 39-48 glucose-6-phosphate isomerase Homo sapiens 70-73 2171349-3 1990 In HEC that had been acid-loaded using nigericin or a NH4Cl prepulse, pHi recovery occurred via a Na(+)-dependent mechanism that was inhibited by 5-(N-ethyl-N-isopropyl)amiloride (EIPA). Ammonium Chloride 54-59 glucose-6-phosphate isomerase Homo sapiens 70-73 2171349-3 1990 In HEC that had been acid-loaded using nigericin or a NH4Cl prepulse, pHi recovery occurred via a Na(+)-dependent mechanism that was inhibited by 5-(N-ethyl-N-isopropyl)amiloride (EIPA). ethylisopropylamiloride 146-178 glucose-6-phosphate isomerase Homo sapiens 70-73 2171349-3 1990 In HEC that had been acid-loaded using nigericin or a NH4Cl prepulse, pHi recovery occurred via a Na(+)-dependent mechanism that was inhibited by 5-(N-ethyl-N-isopropyl)amiloride (EIPA). ethylisopropylamiloride 180-184 glucose-6-phosphate isomerase Homo sapiens 70-73 2171349-8 1990 However, when HEC were acid-loaded in the presence of hypertonic (sucrose) medium, Na(+)-H+ exchange activity (22Na influx or pHi recovery) increased markedly. Sucrose 66-73 glucose-6-phosphate isomerase Homo sapiens 126-129 2147940-2 1990 Lipophosphoglycan (LPG) and glycosyl phosphatidylinositol Ag (GPI), are glycolipids present on the membrane of Leishmania parasites. glycosyl phosphatidylinositol ag 28-60 glucose-6-phosphate isomerase Homo sapiens 62-65 2147940-5 1990 The GPI are smaller glycolipids with a structure resembling the phosphocarbohydrate core of the LPG. Glycolipids 20-31 glucose-6-phosphate isomerase Homo sapiens 4-7 2147940-5 1990 The GPI are smaller glycolipids with a structure resembling the phosphocarbohydrate core of the LPG. phosphocarbohydrate 64-83 glucose-6-phosphate isomerase Homo sapiens 4-7 2147940-5 1990 The GPI are smaller glycolipids with a structure resembling the phosphocarbohydrate core of the LPG. lipophosphonoglycan 96-99 glucose-6-phosphate isomerase Homo sapiens 4-7 2174046-6 1990 However, exposure of growth-arrested VSMC to these agonists for 24 h caused significant differences in pHi: 7.18 (10% serum), 7.16 (platelet-derived growth factor), 6.99 (angiotensin II), and 7.08 (0.4% serum). vsmc 37-41 glucose-6-phosphate isomerase Homo sapiens 103-106 2225375-3 1990 Noninvasive indexes of platelet activation and of platelet/vessel wall interaction (urinary excretion of the 2,3-dinor-metabolites of thromboxane A2 [Tx-M] and prostacyclin [PGI-M], respectively) were analyzed in samples collected in the basal state and after the test. 2,3-dinor 109-118 glucose-6-phosphate isomerase Homo sapiens 174-177 2280254-4 1990 An NH4Cl prepulse was used to lower pHi. Ammonium Chloride 3-8 glucose-6-phosphate isomerase Homo sapiens 36-39 2280254-11 1990 We postulated that low pHi slows glycolysis with its associated ATP formation by reducing glycogenolysis and particularly by reducing conversion of fructose-6-phosphate to fructose-1,6-diphosphate through inhibition of phosphofructokinase (PFK), an enzyme which is known to be highly pH sensitive. Adenosine Triphosphate 64-67 glucose-6-phosphate isomerase Homo sapiens 23-26 2280254-11 1990 We postulated that low pHi slows glycolysis with its associated ATP formation by reducing glycogenolysis and particularly by reducing conversion of fructose-6-phosphate to fructose-1,6-diphosphate through inhibition of phosphofructokinase (PFK), an enzyme which is known to be highly pH sensitive. fructose-6-phosphate 148-168 glucose-6-phosphate isomerase Homo sapiens 23-26 2280254-11 1990 We postulated that low pHi slows glycolysis with its associated ATP formation by reducing glycogenolysis and particularly by reducing conversion of fructose-6-phosphate to fructose-1,6-diphosphate through inhibition of phosphofructokinase (PFK), an enzyme which is known to be highly pH sensitive. fructose-1,6-diphosphate 172-196 glucose-6-phosphate isomerase Homo sapiens 23-26 2170052-2 1990 We investigated in a physiological salt solution (PSS) containing HCO3- the intracellular pH (pHi) regulating mechanisms in smooth muscle cells cultured from human internal mammary arteries, using the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) and 22Na+ influx rates. salt solution 35-48 glucose-6-phosphate isomerase Homo sapiens 94-97 2170052-2 1990 We investigated in a physiological salt solution (PSS) containing HCO3- the intracellular pH (pHi) regulating mechanisms in smooth muscle cells cultured from human internal mammary arteries, using the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) and 22Na+ influx rates. pss 50-53 glucose-6-phosphate isomerase Homo sapiens 94-97 2170052-2 1990 We investigated in a physiological salt solution (PSS) containing HCO3- the intracellular pH (pHi) regulating mechanisms in smooth muscle cells cultured from human internal mammary arteries, using the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) and 22Na+ influx rates. Bicarbonates 66-70 glucose-6-phosphate isomerase Homo sapiens 94-97 2170052-3 1990 The recovery of pHi from an equivalent intracellular acidosis was more rapid when the cells were incubated in CO2/HCO3(-)-buffered PSS than in HEPES-buffered PSS. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 110-113 glucose-6-phosphate isomerase Homo sapiens 16-19 2170052-3 1990 The recovery of pHi from an equivalent intracellular acidosis was more rapid when the cells were incubated in CO2/HCO3(-)-buffered PSS than in HEPES-buffered PSS. Bicarbonates 114-118 glucose-6-phosphate isomerase Homo sapiens 16-19 2170052-3 1990 The recovery of pHi from an equivalent intracellular acidosis was more rapid when the cells were incubated in CO2/HCO3(-)-buffered PSS than in HEPES-buffered PSS. pss 131-134 glucose-6-phosphate isomerase Homo sapiens 16-19 2170052-3 1990 The recovery of pHi from an equivalent intracellular acidosis was more rapid when the cells were incubated in CO2/HCO3(-)-buffered PSS than in HEPES-buffered PSS. HEPES 143-148 glucose-6-phosphate isomerase Homo sapiens 16-19 2170052-3 1990 The recovery of pHi from an equivalent intracellular acidosis was more rapid when the cells were incubated in CO2/HCO3(-)-buffered PSS than in HEPES-buffered PSS. pss 158-161 glucose-6-phosphate isomerase Homo sapiens 16-19 2170052-10 1990 Taken together, the results indicate that Na+/H+ exchange and a previously undescribed EIPA-sensitive Na(+)- and HCO3(-)-dependent mechanism play an important role in regulating the pHi of human vascular smooth muscle. Bicarbonates 113-117 glucose-6-phosphate isomerase Homo sapiens 182-185 2145772-1 1990 We have previously shown that cytoplasmic pH (pHi) recovery in pulmonary macrophages, under nominally HCO3(-)-free conditions, after acute intracellular acidification is Na+ and amiloride insensitive and is blocked by nonspecific proton adenosinetriphosphatase (ATPase) inhibitors N-ethyl-maleimide and N,N"-dicyclohexylcarbodiimide [Am. Bicarbonates 102-107 glucose-6-phosphate isomerase Homo sapiens 46-49 2145772-1 1990 We have previously shown that cytoplasmic pH (pHi) recovery in pulmonary macrophages, under nominally HCO3(-)-free conditions, after acute intracellular acidification is Na+ and amiloride insensitive and is blocked by nonspecific proton adenosinetriphosphatase (ATPase) inhibitors N-ethyl-maleimide and N,N"-dicyclohexylcarbodiimide [Am. Amiloride 178-187 glucose-6-phosphate isomerase Homo sapiens 46-49 2145772-1 1990 We have previously shown that cytoplasmic pH (pHi) recovery in pulmonary macrophages, under nominally HCO3(-)-free conditions, after acute intracellular acidification is Na+ and amiloride insensitive and is blocked by nonspecific proton adenosinetriphosphatase (ATPase) inhibitors N-ethyl-maleimide and N,N"-dicyclohexylcarbodiimide [Am. Ethylmaleimide 281-298 glucose-6-phosphate isomerase Homo sapiens 46-49 2145772-1 1990 We have previously shown that cytoplasmic pH (pHi) recovery in pulmonary macrophages, under nominally HCO3(-)-free conditions, after acute intracellular acidification is Na+ and amiloride insensitive and is blocked by nonspecific proton adenosinetriphosphatase (ATPase) inhibitors N-ethyl-maleimide and N,N"-dicyclohexylcarbodiimide [Am. Dicyclohexylcarbodiimide 303-332 glucose-6-phosphate isomerase Homo sapiens 46-49 2145772-7 1990 Dose-dependent reductions in ATP levels and in the rate of pHi recovery were obtained in the presence of KCN (50% inhibition, 10(-4) M). Potassium Cyanide 105-108 glucose-6-phosphate isomerase Homo sapiens 59-62 2145772-8 1990 Parallel reductions in ATP content and the rate of pHi recovery were noted in the presence of antimycin A, rotenone, oligomycin, and iodoacetate. Antimycin A 94-105 glucose-6-phosphate isomerase Homo sapiens 51-54 2145772-8 1990 Parallel reductions in ATP content and the rate of pHi recovery were noted in the presence of antimycin A, rotenone, oligomycin, and iodoacetate. Rotenone 107-115 glucose-6-phosphate isomerase Homo sapiens 51-54 2145772-8 1990 Parallel reductions in ATP content and the rate of pHi recovery were noted in the presence of antimycin A, rotenone, oligomycin, and iodoacetate. Oligomycins 117-127 glucose-6-phosphate isomerase Homo sapiens 51-54 2145772-8 1990 Parallel reductions in ATP content and the rate of pHi recovery were noted in the presence of antimycin A, rotenone, oligomycin, and iodoacetate. Iodoacetates 133-144 glucose-6-phosphate isomerase Homo sapiens 51-54 2145772-10 1990 The more specific vacuolar H(+)-ATPase inhibitors, bafilomycin A1 and 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, resulted in no decrement in cellular ATP levels but significantly inhibited pHi recovery. 4-Chloro-7-nitrobenzofurazan 70-109 glucose-6-phosphate isomerase Homo sapiens 187-190 2145772-11 1990 These studies demonstrate that recovery from an acid load is ATP dependent and provide support for a plasmalemmal proton ATPase, perhaps of the vacuolar type, that participates in regulation of pHi in pulmonary macrophages. Adenosine Triphosphate 61-64 glucose-6-phosphate isomerase Homo sapiens 194-197 2169196-1 1990 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to determine the effect of ambient CO2-HCO3- on the regulation of intracellular pH (pHi) and the pHi response to arginine vasopressin (AVP) in A10 vascular smooth muscle (VSM) cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 33-79 glucose-6-phosphate isomerase Homo sapiens 181-184 2168414-4 1990 Furthermore, when thrombin (0.1 unit/ml) or the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) had raised pHi from 7.13 +/- 0.05 to 7.35 +/- 0.07 (n = 30), addition of NaF (2.5-10 mM) rapidly restored pHi to values found before stimulation. phorbol ester 12-o-tetradecanoylphorbol 13-acetate 48-98 glucose-6-phosphate isomerase Homo sapiens 116-119 2168414-4 1990 Furthermore, when thrombin (0.1 unit/ml) or the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) had raised pHi from 7.13 +/- 0.05 to 7.35 +/- 0.07 (n = 30), addition of NaF (2.5-10 mM) rapidly restored pHi to values found before stimulation. Tetradecanoylphorbol Acetate 100-103 glucose-6-phosphate isomerase Homo sapiens 116-119 2168414-4 1990 Furthermore, when thrombin (0.1 unit/ml) or the phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) had raised pHi from 7.13 +/- 0.05 to 7.35 +/- 0.07 (n = 30), addition of NaF (2.5-10 mM) rapidly restored pHi to values found before stimulation. Tetradecanoylphorbol Acetate 100-103 glucose-6-phosphate isomerase Homo sapiens 211-214 2396711-0 1990 31P-NMR measurements of pHi and high-energy phosphates in isolated turtle hearts during anoxia and acidosis. ET bromodomain inhibitor 0-3 glucose-6-phosphate isomerase Homo sapiens 24-27 2169196-5 1990 In CO2-HCO3(-)-containing media, amiloride-sensitive Na(+)-H+ exchange mediated 85% of acid extrusion at a pHi of 6.48, but the DIDS-sensitive acid extrusion mechanism (NA(+)-dependent Cl(-)-HCO3- exchange) was the dominant acid extrusion mechanism at a pHi of 6.94. Amiloride 33-42 glucose-6-phosphate isomerase Homo sapiens 107-110 2169196-5 1990 In CO2-HCO3(-)-containing media, amiloride-sensitive Na(+)-H+ exchange mediated 85% of acid extrusion at a pHi of 6.48, but the DIDS-sensitive acid extrusion mechanism (NA(+)-dependent Cl(-)-HCO3- exchange) was the dominant acid extrusion mechanism at a pHi of 6.94. Amiloride 33-42 glucose-6-phosphate isomerase Homo sapiens 254-257 2169196-7 1990 Both amiloride- and DIDS-sensitive processes regulated steady-state pHi in CO2-HCO3-. Amiloride 5-14 glucose-6-phosphate isomerase Homo sapiens 68-71 2169196-1 1990 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to determine the effect of ambient CO2-HCO3- on the regulation of intracellular pH (pHi) and the pHi response to arginine vasopressin (AVP) in A10 vascular smooth muscle (VSM) cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 33-79 glucose-6-phosphate isomerase Homo sapiens 194-197 2169196-7 1990 Both amiloride- and DIDS-sensitive processes regulated steady-state pHi in CO2-HCO3-. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 20-24 glucose-6-phosphate isomerase Homo sapiens 68-71 2169196-7 1990 Both amiloride- and DIDS-sensitive processes regulated steady-state pHi in CO2-HCO3-. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 75-78 glucose-6-phosphate isomerase Homo sapiens 68-71 2169196-1 1990 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to determine the effect of ambient CO2-HCO3- on the regulation of intracellular pH (pHi) and the pHi response to arginine vasopressin (AVP) in A10 vascular smooth muscle (VSM) cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 81-86 glucose-6-phosphate isomerase Homo sapiens 181-184 2169196-7 1990 Both amiloride- and DIDS-sensitive processes regulated steady-state pHi in CO2-HCO3-. Bicarbonates 79-83 glucose-6-phosphate isomerase Homo sapiens 68-71 2169196-1 1990 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to determine the effect of ambient CO2-HCO3- on the regulation of intracellular pH (pHi) and the pHi response to arginine vasopressin (AVP) in A10 vascular smooth muscle (VSM) cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 81-86 glucose-6-phosphate isomerase Homo sapiens 194-197 2169196-9 1990 We conclude that the steady-state pHi, the mechanisms of pHi regulation, and the pHi response to AVP in A10 cells are critically influenced by the presence of extracellular CO2-HCO3-. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 173-176 glucose-6-phosphate isomerase Homo sapiens 34-37 2169196-1 1990 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to determine the effect of ambient CO2-HCO3- on the regulation of intracellular pH (pHi) and the pHi response to arginine vasopressin (AVP) in A10 vascular smooth muscle (VSM) cells. co2-hco3 132-140 glucose-6-phosphate isomerase Homo sapiens 181-184 2169196-9 1990 We conclude that the steady-state pHi, the mechanisms of pHi regulation, and the pHi response to AVP in A10 cells are critically influenced by the presence of extracellular CO2-HCO3-. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 173-176 glucose-6-phosphate isomerase Homo sapiens 57-60 2169196-9 1990 We conclude that the steady-state pHi, the mechanisms of pHi regulation, and the pHi response to AVP in A10 cells are critically influenced by the presence of extracellular CO2-HCO3-. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 173-176 glucose-6-phosphate isomerase Homo sapiens 57-60 2169196-9 1990 We conclude that the steady-state pHi, the mechanisms of pHi regulation, and the pHi response to AVP in A10 cells are critically influenced by the presence of extracellular CO2-HCO3-. Bicarbonates 177-181 glucose-6-phosphate isomerase Homo sapiens 34-37 2169196-9 1990 We conclude that the steady-state pHi, the mechanisms of pHi regulation, and the pHi response to AVP in A10 cells are critically influenced by the presence of extracellular CO2-HCO3-. Bicarbonates 177-181 glucose-6-phosphate isomerase Homo sapiens 57-60 2169196-9 1990 We conclude that the steady-state pHi, the mechanisms of pHi regulation, and the pHi response to AVP in A10 cells are critically influenced by the presence of extracellular CO2-HCO3-. Bicarbonates 177-181 glucose-6-phosphate isomerase Homo sapiens 57-60 2169197-1 1990 The intracellular pH (pHi) dependence of the rate of Na(+)-H+ exchange was determined in undifferentiated promyelocytic HL-60 cells by measuring alkalinization rates using the fluorescent pHi indicator 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF). bcecf 252-257 glucose-6-phosphate isomerase Homo sapiens 22-25 2169196-1 1990 The fluorescent pH-sensitive dye 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein (BCECF) was used to determine the effect of ambient CO2-HCO3- on the regulation of intracellular pH (pHi) and the pHi response to arginine vasopressin (AVP) in A10 vascular smooth muscle (VSM) cells. co2-hco3 132-140 glucose-6-phosphate isomerase Homo sapiens 194-197 2169196-2 1990 Steady-state pHi averaged 7.04 +/- 0.02 in the absence and 7.25 +/- 0.01 in the presence of CO2-HCO3-. Bicarbonates 96-100 glucose-6-phosphate isomerase Homo sapiens 13-16 2382444-4 1990 pHi changes were recorded by computer-assisted spectrofluorimetric monitoring of the pH-sensitive, fluorescent dye BCECF (2",7"-bis(carboxyethyl)- 5(6)carboxyfluorescein). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 115-120 glucose-6-phosphate isomerase Homo sapiens 0-3 2231423-9 1990 pHi was altered using either NH4Cl pulses resulting in small changes in Cai2+ or using a weak acid and base (propionate and trimethylamine), which produced a fall and a rise in Cai2+ respectively. Ammonium Chloride 29-34 glucose-6-phosphate isomerase Homo sapiens 0-3 2231423-9 1990 pHi was altered using either NH4Cl pulses resulting in small changes in Cai2+ or using a weak acid and base (propionate and trimethylamine), which produced a fall and a rise in Cai2+ respectively. cai2+ 72-77 glucose-6-phosphate isomerase Homo sapiens 0-3 2231423-9 1990 pHi was altered using either NH4Cl pulses resulting in small changes in Cai2+ or using a weak acid and base (propionate and trimethylamine), which produced a fall and a rise in Cai2+ respectively. Propionates 109-119 glucose-6-phosphate isomerase Homo sapiens 0-3 2231423-9 1990 pHi was altered using either NH4Cl pulses resulting in small changes in Cai2+ or using a weak acid and base (propionate and trimethylamine), which produced a fall and a rise in Cai2+ respectively. trimethylamine 124-138 glucose-6-phosphate isomerase Homo sapiens 0-3 2231423-9 1990 pHi was altered using either NH4Cl pulses resulting in small changes in Cai2+ or using a weak acid and base (propionate and trimethylamine), which produced a fall and a rise in Cai2+ respectively. cai2+ 177-182 glucose-6-phosphate isomerase Homo sapiens 0-3 2231423-18 1990 It would appear that the mechanisms influencing Cai2+ and pHi are linked. cai2+ 48-53 glucose-6-phosphate isomerase Homo sapiens 58-61 2165610-6 1990 Correcting the total recovery rate for this EIPA-insensitive component, we found that the EIPA-sensitive (Na-H exchange) rate fell steeply with increasing pHi between 6.3 and 6.7 but was relatively pHi insensitive between 6.7 and 7.2. ethylisopropylamiloride 90-94 glucose-6-phosphate isomerase Homo sapiens 155-158 2165610-6 1990 Correcting the total recovery rate for this EIPA-insensitive component, we found that the EIPA-sensitive (Na-H exchange) rate fell steeply with increasing pHi between 6.3 and 6.7 but was relatively pHi insensitive between 6.7 and 7.2. ethylisopropylamiloride 90-94 glucose-6-phosphate isomerase Homo sapiens 198-201 2235293-1 1990 The dependence of intracellular free calcium ([Ca2+]i) and tension on membrane potential and intracellular pH (pHi) was studied in single isolated fibres of the crayfish claw-opener muscle using ion-selective microelectrodes. Calcium 37-44 glucose-6-phosphate isomerase Homo sapiens 111-114 2235293-4 1990 Contractions evoked by an increase extracellular potassium [( K+]0) produced a fall in pHi of 0.01-0.05 units. Potassium 49-58 glucose-6-phosphate isomerase Homo sapiens 87-90 2235293-8 1990 The sensitivity of resting tension to a change in pHi (quantified as -dT/dpHi) showed a progressive increase during a fall in pHi within the range examined (pHi 6.2-7.5). 1,4-androstadiene-3,17-dione 66-72 glucose-6-phosphate isomerase Homo sapiens 50-53 2235293-8 1990 The sensitivity of resting tension to a change in pHi (quantified as -dT/dpHi) showed a progressive increase during a fall in pHi within the range examined (pHi 6.2-7.5). 1,4-androstadiene-3,17-dione 66-72 glucose-6-phosphate isomerase Homo sapiens 74-77 2235293-8 1990 The sensitivity of resting tension to a change in pHi (quantified as -dT/dpHi) showed a progressive increase during a fall in pHi within the range examined (pHi 6.2-7.5). 1,4-androstadiene-3,17-dione 66-72 glucose-6-phosphate isomerase Homo sapiens 74-77 2235293-8 1990 The sensitivity of resting tension to a change in pHi (quantified as -dT/dpHi) showed a progressive increase during a fall in pHi within the range examined (pHi 6.2-7.5). dphi 73-77 glucose-6-phosphate isomerase Homo sapiens 50-53 2235293-9 1990 The pHi/[Ca2+]i and pHi/tension relationships were monotonic throughout the multiphasic pHi change caused by NH4Cl. Ammonium Chloride 109-114 glucose-6-phosphate isomerase Homo sapiens 4-7 2235293-9 1990 The pHi/[Ca2+]i and pHi/tension relationships were monotonic throughout the multiphasic pHi change caused by NH4Cl. Ammonium Chloride 109-114 glucose-6-phosphate isomerase Homo sapiens 20-23 2235293-9 1990 The pHi/[Ca2+]i and pHi/tension relationships were monotonic throughout the multiphasic pHi change caused by NH4Cl. Ammonium Chloride 109-114 glucose-6-phosphate isomerase Homo sapiens 20-23 2382707-1 1990 A dual-excitation inverted confocal laser-scanning microscope has been developed for measuring intracellular pH (pHi) using 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) in individual cells in the isolated perfused cortical collecting tubule (CCT). 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein 124-172 glucose-6-phosphate isomerase Homo sapiens 113-116 2382707-1 1990 A dual-excitation inverted confocal laser-scanning microscope has been developed for measuring intracellular pH (pHi) using 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF) in individual cells in the isolated perfused cortical collecting tubule (CCT). bcecf 174-179 glucose-6-phosphate isomerase Homo sapiens 113-116 1698980-2 1990 The mode of action of gamma-aminobutyric acid (GABA) on intracellular pH (pHi) and surface pH (pHs) was studied in crayfish muscle fibres using H(+)-selective microelectrodes. gamma-Aminobutyric Acid 22-45 glucose-6-phosphate isomerase Homo sapiens 74-77 1698980-2 1990 The mode of action of gamma-aminobutyric acid (GABA) on intracellular pH (pHi) and surface pH (pHs) was studied in crayfish muscle fibres using H(+)-selective microelectrodes. gamma-Aminobutyric Acid 47-51 glucose-6-phosphate isomerase Homo sapiens 74-77 1698980-5 1990 GABA (5 x 10(-6)-10(-3) M) produced a reversible fall in pHi which showed a dependence on the concentrations of both GABA and HCO3-. gamma-Aminobutyric Acid 0-4 glucose-6-phosphate isomerase Homo sapiens 57-60 1698980-5 1990 GABA (5 x 10(-6)-10(-3) M) produced a reversible fall in pHi which showed a dependence on the concentrations of both GABA and HCO3-. gamma-Aminobutyric Acid 117-121 glucose-6-phosphate isomerase Homo sapiens 57-60 1698980-5 1990 GABA (5 x 10(-6)-10(-3) M) produced a reversible fall in pHi which showed a dependence on the concentrations of both GABA and HCO3-. Bicarbonates 126-130 glucose-6-phosphate isomerase Homo sapiens 57-60 1698980-8 1990 In the presence of 30 mM-HCO3-, a near-saturating concentration of GABA (0.5 mM) produced a mean fall in pHi of 0.43 units. Bicarbonates 25-29 glucose-6-phosphate isomerase Homo sapiens 105-108 1698980-8 1990 In the presence of 30 mM-HCO3-, a near-saturating concentration of GABA (0.5 mM) produced a mean fall in pHi of 0.43 units. gamma-Aminobutyric Acid 67-71 glucose-6-phosphate isomerase Homo sapiens 105-108 1698980-9 1990 This change in pHi accounted for about two-thirds of the GABA-induced decrease (from -66 to -29 mV) in the sarcolemmal H+ driving force, while the rest was due to the simultaneous depolarization. gamma-Aminobutyric Acid 57-61 glucose-6-phosphate isomerase Homo sapiens 15-18 1698980-11 1990 The apparent net efflux of HCO3- (JHCO3e) produced by a given concentration of GABA was estimated on the basis of the instantaneous rate of change of pHi. Bicarbonates 27-31 glucose-6-phosphate isomerase Homo sapiens 150-153 1698980-11 1990 The apparent net efflux of HCO3- (JHCO3e) produced by a given concentration of GABA was estimated on the basis of the instantaneous rate of change of pHi. jhco3e 34-40 glucose-6-phosphate isomerase Homo sapiens 150-153 1698980-11 1990 The apparent net efflux of HCO3- (JHCO3e) produced by a given concentration of GABA was estimated on the basis of the instantaneous rate of change of pHi. gamma-Aminobutyric Acid 79-83 glucose-6-phosphate isomerase Homo sapiens 150-153 1698980-19 1990 Application of acetazolamide (10(-4) M) for 5 min or more produced a decrease of up to 60% in the maximum rate of fall of pHi at GABA concentrations higher than 20 microM. Acetazolamide 15-28 glucose-6-phosphate isomerase Homo sapiens 122-125 1698980-19 1990 Application of acetazolamide (10(-4) M) for 5 min or more produced a decrease of up to 60% in the maximum rate of fall of pHi at GABA concentrations higher than 20 microM. gamma-Aminobutyric Acid 129-133 glucose-6-phosphate isomerase Homo sapiens 122-125 1698980-23 1990 This indicates that the maintenance of a non-equilibrium H+ gradient at plateau acidosis and the recovery of pHi are attributable to Na(+)-dependent Cl(-)-HCO3- exchange. Bicarbonates 155-159 glucose-6-phosphate isomerase Homo sapiens 109-112 1698980-25 1990 We conclude that the effects of GABA on pHi and pHs are due to electrodiffusion of HCO3- across postsynaptic anion channels. gamma-Aminobutyric Acid 32-36 glucose-6-phosphate isomerase Homo sapiens 40-43 1698980-25 1990 We conclude that the effects of GABA on pHi and pHs are due to electrodiffusion of HCO3- across postsynaptic anion channels. Bicarbonates 83-87 glucose-6-phosphate isomerase Homo sapiens 40-43 2382444-4 1990 pHi changes were recorded by computer-assisted spectrofluorimetric monitoring of the pH-sensitive, fluorescent dye BCECF (2",7"-bis(carboxyethyl)- 5(6)carboxyfluorescein). 2",7"-bis(carboxyethyl)- 5 122-148 glucose-6-phosphate isomerase Homo sapiens 0-3 2382444-4 1990 pHi changes were recorded by computer-assisted spectrofluorimetric monitoring of the pH-sensitive, fluorescent dye BCECF (2",7"-bis(carboxyethyl)- 5(6)carboxyfluorescein). 6-carboxyfluorescein 151-169 glucose-6-phosphate isomerase Homo sapiens 0-3 2382444-5 1990 Resting pHi in HEPES (N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid) buffered solution was 7.53 +/- 0.01. HEPES 15-20 glucose-6-phosphate isomerase Homo sapiens 8-11 2382444-5 1990 Resting pHi in HEPES (N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid) buffered solution was 7.53 +/- 0.01. HEPES 22-73 glucose-6-phosphate isomerase Homo sapiens 8-11 2382444-6 1990 Intracellular acidification after an ammonium prepulse produced a pHi decline of 0.5 units and pHi returned to normal value in NaCl Ringer"s. Ammonium Compounds 37-45 glucose-6-phosphate isomerase Homo sapiens 66-69 2382444-6 1990 Intracellular acidification after an ammonium prepulse produced a pHi decline of 0.5 units and pHi returned to normal value in NaCl Ringer"s. Sodium Chloride 127-131 glucose-6-phosphate isomerase Homo sapiens 95-98 2382444-9 1990 Na-free solution led to a pHi decrease to 7.39 +/- 0.04 after 16 min, pHi was also lowered by 8 minute incubation of cells with 1 mM amiloride (7.40 +/- 0.02). Amiloride 133-142 glucose-6-phosphate isomerase Homo sapiens 26-29 2382444-9 1990 Na-free solution led to a pHi decrease to 7.39 +/- 0.04 after 16 min, pHi was also lowered by 8 minute incubation of cells with 1 mM amiloride (7.40 +/- 0.02). Amiloride 133-142 glucose-6-phosphate isomerase Homo sapiens 70-73 2382444-10 1990 In HCO3/CO2-buffered solution resting pHi was 7.42 +/- 0.01 (n = 35). Bicarbonates 3-7 glucose-6-phosphate isomerase Homo sapiens 38-41 2382444-10 1990 In HCO3/CO2-buffered solution resting pHi was 7.42 +/- 0.01 (n = 35). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 8-11 glucose-6-phosphate isomerase Homo sapiens 38-41 2382444-11 1990 Recovery from an acute acid load, induced by NH4 prepulse or switching from HEPES- to bicarbonate-buffered solution, was Na dependent, Cl independent, reversible and only partially blocked by 1 mM amiloride - pHi slowly recovered from 6.83 +/- 0.03 to 7.00 +/- 0.06 in 8 minutes. HEPES 76-81 glucose-6-phosphate isomerase Homo sapiens 209-212 2382444-11 1990 Recovery from an acute acid load, induced by NH4 prepulse or switching from HEPES- to bicarbonate-buffered solution, was Na dependent, Cl independent, reversible and only partially blocked by 1 mM amiloride - pHi slowly recovered from 6.83 +/- 0.03 to 7.00 +/- 0.06 in 8 minutes. Bicarbonates 86-97 glucose-6-phosphate isomerase Homo sapiens 209-212 2382444-11 1990 Recovery from an acute acid load, induced by NH4 prepulse or switching from HEPES- to bicarbonate-buffered solution, was Na dependent, Cl independent, reversible and only partially blocked by 1 mM amiloride - pHi slowly recovered from 6.83 +/- 0.03 to 7.00 +/- 0.06 in 8 minutes. Amiloride 197-206 glucose-6-phosphate isomerase Homo sapiens 209-212 2382444-12 1990 In the presence of amiloride and 200 microns H2DIDS (dihydro-4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid) pHi recovery was completely inhibited for 8 minutes. Amiloride 19-28 glucose-6-phosphate isomerase Homo sapiens 113-116 2382444-12 1990 In the presence of amiloride and 200 microns H2DIDS (dihydro-4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid) pHi recovery was completely inhibited for 8 minutes. dihydro-DIDS 45-51 glucose-6-phosphate isomerase Homo sapiens 113-116 2382444-12 1990 In the presence of amiloride and 200 microns H2DIDS (dihydro-4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid) pHi recovery was completely inhibited for 8 minutes. dihydro-4,4"-diisothiocyanatostilbene-2,2"-disulfonic acid 53-111 glucose-6-phosphate isomerase Homo sapiens 113-116 2119394-3 1990 Compared with pHo 7.5, the effects were greater at pHo 6.9, where the concentration of CO2, a permeant acid, was greater and would cause a greater acidification of intracellular pH (pHi). Carbon Dioxide 87-90 glucose-6-phosphate isomerase Homo sapiens 182-185 2115574-8 1990 In low-calcium low-sodium medium (choline substitution for all but 13 mM sodium), acidification with CO2 increased Cai to 425 +/- 35 nM (n = 11) at pHi 5.9-6.0 and gj was reduced to near zero. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 101-104 glucose-6-phosphate isomerase Homo sapiens 148-151 2115574-9 1990 Junctional conductance could also be reduced to near zero at pHi 6.0 in low-calcium medium containing the calcium ionophore, A23187. Calcium 76-83 glucose-6-phosphate isomerase Homo sapiens 61-64 2115574-9 1990 Junctional conductance could also be reduced to near zero at pHi 6.0 in low-calcium medium containing the calcium ionophore, A23187. Calcimycin 125-131 glucose-6-phosphate isomerase Homo sapiens 61-64 2159481-13 1990 Ethylisopropylamiloride, a specific inhibitor of the plasma membrane Na+/H+ antiporter, completely suppressed the effects of FN on both pHi and DNA synthesis. ethylisopropylamiloride 0-23 glucose-6-phosphate isomerase Homo sapiens 136-139 2196684-10 1990 The roles that blood pressure, glucose, temperature, or the administration of extrinsic buffers and drugs have on modulating the severity of and relationship between changes in blood flow, energy metabolites, or pHi, are all amenable to study using in vivo MRS. Glucose 31-38 glucose-6-phosphate isomerase Homo sapiens 212-215 2191723-7 1990 The modulators of vincristine sensitivity had no immediate effect on pHi, whereas after 3 days of incubation ionomycin and forskolin tended to increase pHi. Ionomycin 109-118 glucose-6-phosphate isomerase Homo sapiens 152-155 2191723-7 1990 The modulators of vincristine sensitivity had no immediate effect on pHi, whereas after 3 days of incubation ionomycin and forskolin tended to increase pHi. Colforsin 123-132 glucose-6-phosphate isomerase Homo sapiens 152-155 2372106-1 1990 Derivatives of fluorescein sensitive to pH are extensively utilized for the determination of intracellular pH (pHi). Fluorescein 15-26 glucose-6-phosphate isomerase Homo sapiens 111-114 2372106-3 1990 We examined the fluorescein derivative, 5 (and 6)-carboxy-2",7"-dichlorofluorescein (CDCF) for its potential in the microspectrofluorometric measurement of pHi during acidic conditions. Fluorescein 16-27 glucose-6-phosphate isomerase Homo sapiens 156-159 2372106-3 1990 We examined the fluorescein derivative, 5 (and 6)-carboxy-2",7"-dichlorofluorescein (CDCF) for its potential in the microspectrofluorometric measurement of pHi during acidic conditions. 5 (and 6)-carboxy-2",7"-dichlorofluorescein 40-83 glucose-6-phosphate isomerase Homo sapiens 156-159 2372106-3 1990 We examined the fluorescein derivative, 5 (and 6)-carboxy-2",7"-dichlorofluorescein (CDCF) for its potential in the microspectrofluorometric measurement of pHi during acidic conditions. 5-Carboxy-2',7'-dichlorofluorescein 85-89 glucose-6-phosphate isomerase Homo sapiens 156-159 2105226-6 1990 Experiments were carried out altering pHi using trimethylamine, propionate, and ammonium chloride to determine whether pHi could influence Cai2+. cai2+ 139-144 glucose-6-phosphate isomerase Homo sapiens 119-122 2316641-2 1990 pHi was measured using 2",7"-bis(2-carboxyethyl-5,6-carboxyfluorescein (BCECF), an internalized fluorescent pH indicator. 2",7"-bis(2-carboxyethyl-5,6-carboxyfluorescein 23-70 glucose-6-phosphate isomerase Homo sapiens 0-3 2316641-2 1990 pHi was measured using 2",7"-bis(2-carboxyethyl-5,6-carboxyfluorescein (BCECF), an internalized fluorescent pH indicator. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 72-77 glucose-6-phosphate isomerase Homo sapiens 0-3 2316641-6 1990 TPA (a tumor-promoting phorbol ester) and OAG (synthetic diacylglycerol), both potent activators of protein kinase C, imitate the action of EGF in rapidly elevating pHi and stimulating cell growth in thyroid cells. Tetradecanoylphorbol Acetate 0-3 glucose-6-phosphate isomerase Homo sapiens 165-168 2316641-6 1990 TPA (a tumor-promoting phorbol ester) and OAG (synthetic diacylglycerol), both potent activators of protein kinase C, imitate the action of EGF in rapidly elevating pHi and stimulating cell growth in thyroid cells. 1-oleoyl-2-acetylglycerol 42-45 glucose-6-phosphate isomerase Homo sapiens 165-168 2316641-6 1990 TPA (a tumor-promoting phorbol ester) and OAG (synthetic diacylglycerol), both potent activators of protein kinase C, imitate the action of EGF in rapidly elevating pHi and stimulating cell growth in thyroid cells. Diglycerides 57-71 glucose-6-phosphate isomerase Homo sapiens 165-168 2105226-0 1990 Relationship between intracellular pH (pHi) and calcium (Cai2+) in avian heart fibroblasts. Calcium 48-55 glucose-6-phosphate isomerase Homo sapiens 39-42 18592594-1 1990 A mathematical model for the effects of NH(4)Cl addition to the growth medium on hybridoma cell intracellular pH (pH(i)) is presented which includes a detailed description of the properties of the Na(+)/H(+) exchanger. Ammonium Chloride 40-47 glucose-6-phosphate isomerase Homo sapiens 114-119 18592594-2 1990 The model is used to calculate the steady-state value of pH(i) as a function of the extracellular NH(4)Cl concentration, employing parameter values taken from the literature except for the cell permeability to NH(4) (+) This parameter value, estimated to be 3.5 x 10(-7) cm/s, is obtained by fitting simulation results to experimental data obtained previously for the steady-state value of pH(i) in the presence of 10mM NH(4)Cl. (4)cl 100-105 glucose-6-phosphate isomerase Homo sapiens 57-62 18592594-2 1990 The model is used to calculate the steady-state value of pH(i) as a function of the extracellular NH(4)Cl concentration, employing parameter values taken from the literature except for the cell permeability to NH(4) (+) This parameter value, estimated to be 3.5 x 10(-7) cm/s, is obtained by fitting simulation results to experimental data obtained previously for the steady-state value of pH(i) in the presence of 10mM NH(4)Cl. Ammonium Chloride 98-105 glucose-6-phosphate isomerase Homo sapiens 57-62 18592594-3 1990 The model simulates well the major features of previously observed experimental pH(i) changes following NH(4)Cl addition: the transient characteristics, dependence on the NH(4)Cl concentration, and lack of dependence on the external pH value. (4)cl 106-111 glucose-6-phosphate isomerase Homo sapiens 80-85 2347119-0 1990 Sodium-dependent, ethylisopropylamiloride-sensitive mechanisms regulate intracellular pH in human vascular smooth muscle. Sodium 0-6 glucose-6-phosphate isomerase Homo sapiens 86-88 2347119-0 1990 Sodium-dependent, ethylisopropylamiloride-sensitive mechanisms regulate intracellular pH in human vascular smooth muscle. ethylisopropylamiloride 18-41 glucose-6-phosphate isomerase Homo sapiens 86-88 2347119-2 1990 The pH-sensitive dye 2,7-biscarboxy-ethyl-5(6)-carboxyfluorescein (BCECF) was used to examine the contribution of Na(+)-H+ exchange and bicarbonate-dependent processes to intracellular pH (pHi) regulation in cultured human vascular smooth muscle. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 21-65 glucose-6-phosphate isomerase Homo sapiens 4-6 2347119-2 1990 The pH-sensitive dye 2,7-biscarboxy-ethyl-5(6)-carboxyfluorescein (BCECF) was used to examine the contribution of Na(+)-H+ exchange and bicarbonate-dependent processes to intracellular pH (pHi) regulation in cultured human vascular smooth muscle. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 21-65 glucose-6-phosphate isomerase Homo sapiens 185-187 2347119-4 1990 The recovery of pHi following an NH4Cl-induced acidosis was Na(+)-dependent and could be inhibited by ethylisopropylamiloride (200 mumols/L). Ammonium Chloride 33-38 glucose-6-phosphate isomerase Homo sapiens 16-19 2347119-4 1990 The recovery of pHi following an NH4Cl-induced acidosis was Na(+)-dependent and could be inhibited by ethylisopropylamiloride (200 mumols/L). ethylisopropylamiloride 102-125 glucose-6-phosphate isomerase Homo sapiens 16-19 2141113-2 1990 Changes in pHi were estimated (i) from the H+ efflux across the plasma membrane using an extracellular pH electrode and (ii) using an intracellular pH-sensitive fluorescent dye (BCECF). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 178-183 glucose-6-phosphate isomerase Homo sapiens 11-14 2141113-6 1990 After blockade of Na+/H+ exchange by 100 microM ethylisopropylamiloride (EIPA), thrombin induced a decrease in pHi the rate of which averaged 0.39 unit/min in HCO3(-)-containing medium, and 0.57 unit/min in HCO3(-)-free medium. ethylisopropylamiloride 73-77 glucose-6-phosphate isomerase Homo sapiens 111-114 2141113-6 1990 After blockade of Na+/H+ exchange by 100 microM ethylisopropylamiloride (EIPA), thrombin induced a decrease in pHi the rate of which averaged 0.39 unit/min in HCO3(-)-containing medium, and 0.57 unit/min in HCO3(-)-free medium. Bicarbonates 159-166 glucose-6-phosphate isomerase Homo sapiens 111-114 2141113-6 1990 After blockade of Na+/H+ exchange by 100 microM ethylisopropylamiloride (EIPA), thrombin induced a decrease in pHi the rate of which averaged 0.39 unit/min in HCO3(-)-containing medium, and 0.57 unit/min in HCO3(-)-free medium. Bicarbonates 159-163 glucose-6-phosphate isomerase Homo sapiens 111-114 2105226-7 1990 It was found that an intracellular acidification induced a fall in Cai2+ and any rise in pHi induced a rise in Cai2+. cai2+ 111-116 glucose-6-phosphate isomerase Homo sapiens 89-92 2105226-8 1990 These results suggest a direct interaction between Cai2+ and pHi. cai2+ 51-56 glucose-6-phosphate isomerase Homo sapiens 61-64 2155021-5 1990 pHi recovery after intracellular acid loading was partially dependent on the presence of Na+ in the extracellular medium, and was partially inhibited by the Na+/H+ antiport inhibitor, amiloride. Amiloride 184-193 glucose-6-phosphate isomerase Homo sapiens 0-3 2155021-8 1990 40% of total pHi recovery was insensitive to amiloride and independent of extracellular Na+. Amiloride 45-54 glucose-6-phosphate isomerase Homo sapiens 13-16 2155021-9 1990 In both RES and ELI MOs, stimulation with TPA resulted in a biphasic pHi response: an initial acidification followed by a sustained alkalinization to a new steady-state pHi. Tetradecanoylphorbol Acetate 42-45 glucose-6-phosphate isomerase Homo sapiens 69-72 2155021-9 1990 In both RES and ELI MOs, stimulation with TPA resulted in a biphasic pHi response: an initial acidification followed by a sustained alkalinization to a new steady-state pHi. Tetradecanoylphorbol Acetate 42-45 glucose-6-phosphate isomerase Homo sapiens 169-172 2155021-11 1990 The new steady-state pHi attained after TPA stimulation was equivalent in RES and ELI MOs (7.28 +/- 0.04 and 7.31 +/- 0.06, respectively), indicating comparable stimulated Na+/H+ antiport activity. Tetradecanoylphorbol Acetate 40-43 glucose-6-phosphate isomerase Homo sapiens 21-24 2155021-13 1990 The specific NADPH oxidase inhibitor diphenylene iodonium (DPI) completely inhibited the respiratory burst but reduced the magnitude of this pHi reduction by only about 50%. diphenyleneiodonium 37-57 glucose-6-phosphate isomerase Homo sapiens 141-144 2155021-13 1990 The specific NADPH oxidase inhibitor diphenylene iodonium (DPI) completely inhibited the respiratory burst but reduced the magnitude of this pHi reduction by only about 50%. diphenyleneiodonium 59-62 glucose-6-phosphate isomerase Homo sapiens 141-144 2155021-14 1990 This suggested that the TPA-induced pHi reduction was due in part to acid produced via the respiratory burst, and in part to other acid-generating pathways stimulated by TPA. Tetradecanoylphorbol Acetate 24-27 glucose-6-phosphate isomerase Homo sapiens 36-39 2155021-14 1990 This suggested that the TPA-induced pHi reduction was due in part to acid produced via the respiratory burst, and in part to other acid-generating pathways stimulated by TPA. Tetradecanoylphorbol Acetate 170-173 glucose-6-phosphate isomerase Homo sapiens 36-39 2107595-3 1990 We addressed this by analyzing the urinary excretion of the 2,3-dinor-metabolites of TxA2 (Tx-M) and PGI2 (PGI-M) in 10 patients with severe atherosclerosis during 10 consecutive days. 2,3-dinor 60-69 glucose-6-phosphate isomerase Homo sapiens 101-104 2164168-2 1990 We found that synaptosomes isolated in sucrose media have a pHi of 6.77 +/- 0.04 and a [Ca2+]i of about 260 nM, whereas synaptosomes isolated in Na(+)-rich ionic media have a pHi of 6.96 +/- 0.07 and a [Ca2+]i of 463 nM, but both types of preparations have similar membrane potentials of about -50 mV when placed in choline media. Sucrose 39-46 glucose-6-phosphate isomerase Homo sapiens 60-63 2153936-9 1990 Here I present evidence that a decrease in intracellular pH (pHi) markedly reduces the inhibitory effect of ATP on these channels in excised patches from frog skeletal muscle. Adenosine Triphosphate 108-111 glucose-6-phosphate isomerase Homo sapiens 61-64 2154192-3 1990 The fate of D-[2-13C]glucose 6-phosphate exposed to phosphoglucoisomerase (glucose-6-phosphate isomerase, EC 5.3.1.9) in 2H2O was monitored by 13C-n.m.r. d-[2-13c]glucose 6-phosphate 12-40 glucose-6-phosphate isomerase Homo sapiens 75-104 2154192-3 1990 The fate of D-[2-13C]glucose 6-phosphate exposed to phosphoglucoisomerase (glucose-6-phosphate isomerase, EC 5.3.1.9) in 2H2O was monitored by 13C-n.m.r. 2h2o 121-125 glucose-6-phosphate isomerase Homo sapiens 75-104 2154192-3 1990 The fate of D-[2-13C]glucose 6-phosphate exposed to phosphoglucoisomerase (glucose-6-phosphate isomerase, EC 5.3.1.9) in 2H2O was monitored by 13C-n.m.r. 13c 17-20 glucose-6-phosphate isomerase Homo sapiens 75-104 2153112-1 1990 According to recent observations ADP stimulates platelets via activation of Na+/H+ exchange which increases cytosolic pH (pHi). Adenosine Diphosphate 33-36 glucose-6-phosphate isomerase Homo sapiens 122-125 2153112-4 1990 We found that ADP (5 microM) increased pHi from 7.15 +/- 0.08 to 7.35 +/- 0.04 (n = 8) in 2"-7"-bis-(carboxyethyl)-5,6-carboxyfluorescein-loaded platelets, whereas thromboxane A2 formation was inhibited by indomethacin. Adenosine Diphosphate 14-17 glucose-6-phosphate isomerase Homo sapiens 39-42 2153112-4 1990 We found that ADP (5 microM) increased pHi from 7.15 +/- 0.08 to 7.35 +/- 0.04 (n = 8) in 2"-7"-bis-(carboxyethyl)-5,6-carboxyfluorescein-loaded platelets, whereas thromboxane A2 formation was inhibited by indomethacin. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 90-137 glucose-6-phosphate isomerase Homo sapiens 39-42 2153112-8 1990 This inhibition could be completely overcome by artificially raising pHi using either NH4Cl or the Na+/H+ ionophore monensin. Ammonium Chloride 86-91 glucose-6-phosphate isomerase Homo sapiens 69-72 2153112-8 1990 This inhibition could be completely overcome by artificially raising pHi using either NH4Cl or the Na+/H+ ionophore monensin. Monensin 116-124 glucose-6-phosphate isomerase Homo sapiens 69-72 2153112-11 1990 Again, artificially elevating pHi restored U 46619-induced Ca2+ mobilization despite the presence of EIPA. ethylisopropylamiloride 101-105 glucose-6-phosphate isomerase Homo sapiens 30-33 2154219-0 1990 Autocrine motility factor stimulates a three-fold increase in inositol trisphosphate in human melanoma cells. inositol 1,2,3-trisphosphate 62-84 glucose-6-phosphate isomerase Homo sapiens 0-25 2154219-2 1990 Autocrine motility factor (AMF), which is produced by and stimulates motility in A2058 human melanoma cells, was used to characterize phosphoinositide (PtdIns) metabolism activated in association with tumor cell motility. Phosphatidylinositols 134-150 glucose-6-phosphate isomerase Homo sapiens 0-25 2154219-2 1990 Autocrine motility factor (AMF), which is produced by and stimulates motility in A2058 human melanoma cells, was used to characterize phosphoinositide (PtdIns) metabolism activated in association with tumor cell motility. Phosphatidylinositols 134-150 glucose-6-phosphate isomerase Homo sapiens 27-30 2154219-2 1990 Autocrine motility factor (AMF), which is produced by and stimulates motility in A2058 human melanoma cells, was used to characterize phosphoinositide (PtdIns) metabolism activated in association with tumor cell motility. Phosphatidylinositols 152-158 glucose-6-phosphate isomerase Homo sapiens 0-25 2154219-2 1990 Autocrine motility factor (AMF), which is produced by and stimulates motility in A2058 human melanoma cells, was used to characterize phosphoinositide (PtdIns) metabolism activated in association with tumor cell motility. Phosphatidylinositols 152-158 glucose-6-phosphate isomerase Homo sapiens 27-30 2154219-3 1990 AMF stimulated up to a 400% increase in de novo incorporation of 3H-myo-inositol into cellular lipids beginning 40 minutes after exposure. 3h-myo-inositol 65-80 glucose-6-phosphate isomerase Homo sapiens 0-3 2154219-4 1990 In cells prelabeled with 3H-myo-inositol, AMF stimulated a 200% increase in total inositol phosphates (inositol monophosphate, InsP1; inositol bisphosphate, InsP2; inositol trisphosphate, InsP3) after 90 minutes of exposure, with a 300% maximal increase in InsP3 at 120 minutes. 3h-myo-inositol 25-40 glucose-6-phosphate isomerase Homo sapiens 42-45 2154219-4 1990 In cells prelabeled with 3H-myo-inositol, AMF stimulated a 200% increase in total inositol phosphates (inositol monophosphate, InsP1; inositol bisphosphate, InsP2; inositol trisphosphate, InsP3) after 90 minutes of exposure, with a 300% maximal increase in InsP3 at 120 minutes. Inositol Phosphates 82-101 glucose-6-phosphate isomerase Homo sapiens 42-45 2107595-8 1990 Thus, during the last 5 days of ASA treatment the median excretion of Tx-M was depressed (p less than 0.001) to 148 (range 48-428) pg/mg creatinine, while that of PGI-M was decreased (p less than 0.01) to 313 (range 42-2658) pg/mg creatinine. Aspirin 32-35 glucose-6-phosphate isomerase Homo sapiens 163-166 2154219-4 1990 In cells prelabeled with 3H-myo-inositol, AMF stimulated a 200% increase in total inositol phosphates (inositol monophosphate, InsP1; inositol bisphosphate, InsP2; inositol trisphosphate, InsP3) after 90 minutes of exposure, with a 300% maximal increase in InsP3 at 120 minutes. Inositol Phosphates 103-125 glucose-6-phosphate isomerase Homo sapiens 42-45 2154219-4 1990 In cells prelabeled with 3H-myo-inositol, AMF stimulated a 200% increase in total inositol phosphates (inositol monophosphate, InsP1; inositol bisphosphate, InsP2; inositol trisphosphate, InsP3) after 90 minutes of exposure, with a 300% maximal increase in InsP3 at 120 minutes. inositol bisphosphate 134-155 glucose-6-phosphate isomerase Homo sapiens 42-45 2154219-4 1990 In cells prelabeled with 3H-myo-inositol, AMF stimulated a 200% increase in total inositol phosphates (inositol monophosphate, InsP1; inositol bisphosphate, InsP2; inositol trisphosphate, InsP3) after 90 minutes of exposure, with a 300% maximal increase in InsP3 at 120 minutes. inositol 1,2,3-trisphosphate 164-186 glucose-6-phosphate isomerase Homo sapiens 42-45 2154219-6 1990 Treatment with AMF stimulated a parallel dose-dependent increase in both motility and PtdIns levels. Phosphatidylinositols 86-92 glucose-6-phosphate isomerase Homo sapiens 15-18 2180402-1 1990 The effect of endothelin (ET) on the intracellular pH (pHi) of vascular smooth muscle cells (VSMC), was investigated using a fluorescent pH indicator 2",7"-bis(carboxyethyl)carboxyfluorescein (BCECF). vsmc 93-97 glucose-6-phosphate isomerase Homo sapiens 55-58 2154219-10 1990 These data indicate that AMF elicits increases in cell motility and phosphoinositide metabolism via a PT-sensitive G protein signal transduction pathway. Phosphatidylinositols 68-84 glucose-6-phosphate isomerase Homo sapiens 25-28 2089888-7 1990 When pHi approaches normal there will be a net extrusion of Na+, taking osmotic water with it, and presumably with passive washout of lactate. Water 80-85 glucose-6-phosphate isomerase Homo sapiens 5-8 2089888-7 1990 When pHi approaches normal there will be a net extrusion of Na+, taking osmotic water with it, and presumably with passive washout of lactate. Lactic Acid 134-141 glucose-6-phosphate isomerase Homo sapiens 5-8 2136803-13 1990 GPI-PLC treatment of neutrophils indicated that all of this material is phosphatidyl-inositol linked. Phosphatidylinositols 72-93 glucose-6-phosphate isomerase Homo sapiens 0-3 2180402-1 1990 The effect of endothelin (ET) on the intracellular pH (pHi) of vascular smooth muscle cells (VSMC), was investigated using a fluorescent pH indicator 2",7"-bis(carboxyethyl)carboxyfluorescein (BCECF). 2",7"-bis(carboxyethyl)carboxyfluorescein 150-191 glucose-6-phosphate isomerase Homo sapiens 55-58 2180402-1 1990 The effect of endothelin (ET) on the intracellular pH (pHi) of vascular smooth muscle cells (VSMC), was investigated using a fluorescent pH indicator 2",7"-bis(carboxyethyl)carboxyfluorescein (BCECF). bcecf 193-198 glucose-6-phosphate isomerase Homo sapiens 55-58 33764767-2 2021 Taking the [Ca24Al28O64]4+:4e- electride as an example, we here propose a new computational scheme of Phi and, further, characterize Phi of the bulk derivative structures of the electride for clarifying the relationship between structural characteristics and Phi. ca24al28o64]4+ 12-26 glucose-6-phosphate isomerase Homo sapiens 102-105 2109061-5 1990 In the presence of physiological HCO3- and CO2, basal pHi was 7.02 +/- 0.04 compared with 7.15 +/- 0.05 in the absence of HCO3-. Bicarbonates 33-37 glucose-6-phosphate isomerase Homo sapiens 54-57 2109061-5 1990 In the presence of physiological HCO3- and CO2, basal pHi was 7.02 +/- 0.04 compared with 7.15 +/- 0.05 in the absence of HCO3-. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 43-46 glucose-6-phosphate isomerase Homo sapiens 54-57 2109061-6 1990 Estimated cytosolic buffering power was reduced from 35.6 +/- 3.0 to 14.5 +/- 0.4 mM/pH unit by the omission of HCO3-. Bicarbonates 112-116 glucose-6-phosphate isomerase Homo sapiens 85-87 2109061-10 1990 The final pHi was 0.10 +/- 0.01 units above basal in the presence of HCO3- and 0.08 +/- 0.02 units above basal in the absence of HCO3-. Bicarbonates 69-73 glucose-6-phosphate isomerase Homo sapiens 10-13 2109061-14 1990 Replacement of extracellular Na+ with N-methyl-D-glucamine resulted in a fall in basal pHi and abolished recovery from thrombin-evoked acidification in both the presence and absence of HCO3-. Meglumine 38-58 glucose-6-phosphate isomerase Homo sapiens 87-90 2109061-17 1990 However, with PAF and ADP, the subsequent recovery in pHi was slow and did not rise above basal levels. Platelet Activating Factor 14-17 glucose-6-phosphate isomerase Homo sapiens 54-57 2109061-17 1990 However, with PAF and ADP, the subsequent recovery in pHi was slow and did not rise above basal levels. Adenosine Diphosphate 22-25 glucose-6-phosphate isomerase Homo sapiens 54-57 2109061-18 1990 Phorbol dibutyrate, an activator of protein kinase C, evoked a similar elevation in pHi of 0.04 +/- 0.01 units over 3 min in the presence and absence of HCO3-. Phorbol 12,13-Dibutyrate 0-18 glucose-6-phosphate isomerase Homo sapiens 84-87 33764767-2 2021 Taking the [Ca24Al28O64]4+:4e- electride as an example, we here propose a new computational scheme of Phi and, further, characterize Phi of the bulk derivative structures of the electride for clarifying the relationship between structural characteristics and Phi. 4e- electride 27-40 glucose-6-phosphate isomerase Homo sapiens 102-105 34308742-6 2021 The treatment effect of taxol varied in monolayer and tumor spheroids and pHi changes were able to reflect these difference. Paclitaxel 24-29 glucose-6-phosphate isomerase Homo sapiens 74-77 30576235-1 2019 TWIK-related two-pore domain K+ channels (TREKs) are activated by acidic intracellular pH (pHi), membrane stretch, temperature, and arachidonic acid (AA). Arachidonic Acid 132-148 glucose-6-phosphate isomerase Homo sapiens 91-94 30576235-10 2019 In contrast, combined neutralization of pHi-sensing Glu (E332A/R355-7A) induced tonic high current and no further activation by pHi. Glutamic Acid 52-55 glucose-6-phosphate isomerase Homo sapiens 40-43 30576235-11 2019 Interestingly, when the Gly between K330/E332 and R3-pCt was mutated (G334A), hTREK-2 was tonic activated with reversed responses to ATP and acidic pHi. Glycine 24-27 glucose-6-phosphate isomerase Homo sapiens 148-151 34976860-3 2021 Phosphoglucose isomerase (PGI) catalyzes the reversible conversion between glucose-6-phosphate and fructose-6-phosphate, thus acting as a key node for glycolysis, pentose phosphate pathway, and cell wall biosynthesis in fungi. Glucose 75-82 glucose-6-phosphate isomerase Homo sapiens 26-29 34976860-3 2021 Phosphoglucose isomerase (PGI) catalyzes the reversible conversion between glucose-6-phosphate and fructose-6-phosphate, thus acting as a key node for glycolysis, pentose phosphate pathway, and cell wall biosynthesis in fungi. fructose-6-phosphate 99-119 glucose-6-phosphate isomerase Homo sapiens 26-29 34976860-3 2021 Phosphoglucose isomerase (PGI) catalyzes the reversible conversion between glucose-6-phosphate and fructose-6-phosphate, thus acting as a key node for glycolysis, pentose phosphate pathway, and cell wall biosynthesis in fungi. Pentosephosphates 163-180 glucose-6-phosphate isomerase Homo sapiens 26-29 34976860-4 2021 In this study, we constructed an A. flavus pgi deletion mutant, which exhibited specific carbon requirement for survival, reduced conidiation, and slowed germination even under optimal experimental conditions. Carbon 89-95 glucose-6-phosphate isomerase Homo sapiens 43-46 34683262-8 2021 Studying the relationship between the ratio of oil-to-water flow rates (Phi) found that increasing Phi resulted in smaller droplets and an enhanced droplet generation rate. Oils 47-50 glucose-6-phosphate isomerase Homo sapiens 72-75 34683262-8 2021 Studying the relationship between the ratio of oil-to-water flow rates (Phi) found that increasing Phi resulted in smaller droplets and an enhanced droplet generation rate. Oils 47-50 glucose-6-phosphate isomerase Homo sapiens 99-102 34683262-8 2021 Studying the relationship between the ratio of oil-to-water flow rates (Phi) found that increasing Phi resulted in smaller droplets and an enhanced droplet generation rate. Water 54-59 glucose-6-phosphate isomerase Homo sapiens 72-75 34683262-8 2021 Studying the relationship between the ratio of oil-to-water flow rates (Phi) found that increasing Phi resulted in smaller droplets and an enhanced droplet generation rate. Water 54-59 glucose-6-phosphate isomerase Homo sapiens 99-102 34712400-9 2021 Mechanistic evaluation showed that artenimol interfered with the interaction between the SFTSV nucleoprotein and the host glucose-6-phosphate isomerase (GPI), and that omacetaxine mepesuccinate interfered with the interaction between the viral nucleoprotein with the host ribosomal protein L3 (RPL3). artenimol 35-44 glucose-6-phosphate isomerase Homo sapiens 122-151 34712400-9 2021 Mechanistic evaluation showed that artenimol interfered with the interaction between the SFTSV nucleoprotein and the host glucose-6-phosphate isomerase (GPI), and that omacetaxine mepesuccinate interfered with the interaction between the viral nucleoprotein with the host ribosomal protein L3 (RPL3). artenimol 35-44 glucose-6-phosphate isomerase Homo sapiens 153-156 34956382-5 2021 ZJP significantly ameliorate the pathological injury of gastric tissue, increase levels of PGI and PGI/II, and reduce the expression level of proinflammatory factors. zjp 0-3 glucose-6-phosphate isomerase Homo sapiens 91-94 34308742-9 2021 These findings elucidate the significance of pHi in the mechanisms of taxol action on cervical cancer cells and will be valuable for development of new approaches for cancer treatment. Paclitaxel 70-75 glucose-6-phosphate isomerase Homo sapiens 45-48 35603428-1 2022 Glycosylphosphatidylinositols (GPIs) are glycolipids that anchor many proteins (GPI-APs) on the cell surface. Glycosylphosphatidylinositols 0-29 glucose-6-phosphate isomerase Homo sapiens 80-83 34189282-4 2021 Since the apical HCO3 - efflux through CFTR often required the intracellular H+/HCO3 - production and/or the Na+-dependent basolateral HCO3 - uptake, the intracellular pH (pHi) balance might be disturbed, especially as a consequence of increased endogenous H+ and HCO3 - production. Bicarbonates 17-21 glucose-6-phosphate isomerase Homo sapiens 172-175 34189282-4 2021 Since the apical HCO3 - efflux through CFTR often required the intracellular H+/HCO3 - production and/or the Na+-dependent basolateral HCO3 - uptake, the intracellular pH (pHi) balance might be disturbed, especially as a consequence of increased endogenous H+ and HCO3 - production. Bicarbonates 264-270 glucose-6-phosphate isomerase Homo sapiens 172-175 35358527-7 2022 The evident increases in the glucose 1-phosphate and fructose 6-phosphate levels in HepG2 cells were proposed to be induced by the promotion of PGM1/PGM2 and GPI gene expression and the suppression of UPG2 and GFPT1/GFPT2 gene expression. Glucose 29-36 glucose-6-phosphate isomerase Homo sapiens 158-161 35358527-7 2022 The evident increases in the glucose 1-phosphate and fructose 6-phosphate levels in HepG2 cells were proposed to be induced by the promotion of PGM1/PGM2 and GPI gene expression and the suppression of UPG2 and GFPT1/GFPT2 gene expression. fructose-6-phosphate 53-73 glucose-6-phosphate isomerase Homo sapiens 158-161 35613869-1 2022 Combination treatment using fingolimod (FTY720), an immunomodulator, and a pathogenic antigen prevents the progression of glucose-6-phosphate isomerase (GPI)325-339-induced arthritis. Fingolimod Hydrochloride 28-38 glucose-6-phosphate isomerase Homo sapiens 122-151 35613869-1 2022 Combination treatment using fingolimod (FTY720), an immunomodulator, and a pathogenic antigen prevents the progression of glucose-6-phosphate isomerase (GPI)325-339-induced arthritis. Fingolimod Hydrochloride 40-46 glucose-6-phosphate isomerase Homo sapiens 122-151 34219652-4 2021 Na+/H+-exchange elevates pHi preferentially in estrogen receptor-negative breast carcinomas, whereas Na+,HCO3--cotransport raises pHi more in invasive lobular than ductal breast carcinomas and in higher malignancy grade breast cancer. Bicarbonates 105-109 glucose-6-phosphate isomerase Homo sapiens 130-133 34219652-6 2021 Increased dependency on Na+,HCO3--cotransport associates with severe breast cancer: enlarged CO2/HCO3--dependent rises in pHi predict accelerated cell proliferation; whereas enhanced CO2/HCO3--dependent net acid extrusion, elevated NBCn1 protein expression, and reduced NHE1 protein expression predict lymph node metastasis. Bicarbonates 28-32 glucose-6-phosphate isomerase Homo sapiens 122-125 34219652-6 2021 Increased dependency on Na+,HCO3--cotransport associates with severe breast cancer: enlarged CO2/HCO3--dependent rises in pHi predict accelerated cell proliferation; whereas enhanced CO2/HCO3--dependent net acid extrusion, elevated NBCn1 protein expression, and reduced NHE1 protein expression predict lymph node metastasis. Carbon Dioxide 93-96 glucose-6-phosphate isomerase Homo sapiens 122-125 34219652-6 2021 Increased dependency on Na+,HCO3--cotransport associates with severe breast cancer: enlarged CO2/HCO3--dependent rises in pHi predict accelerated cell proliferation; whereas enhanced CO2/HCO3--dependent net acid extrusion, elevated NBCn1 protein expression, and reduced NHE1 protein expression predict lymph node metastasis. Bicarbonates 97-101 glucose-6-phosphate isomerase Homo sapiens 122-125 34219652-9 2021 NBCn1 expression and dependency on Na+,HCO3--cotransport for pHi regulation, measured in biopsies of human primary breast carcinomas, independently predict proliferative activity, lymph node metastasis, and patient survival. Bicarbonates 39-43 glucose-6-phosphate isomerase Homo sapiens 61-64 34143606-3 2021 Recent work suggests that production of l-2-hydroxyglutarte in cancer cells can be regulated by environmental changes, including hypoxia and intracellular pH (pHi). l-2-hydroxyglutarte 40-59 glucose-6-phosphate isomerase Homo sapiens 159-162 35624145-3 2022 Thirteen kinases were found to activate sodium/hydrogen exchanger (NHE1) in normal hematopoietic progenitors, of which FLT3-ITD, KRASG12D, and BTK phosphorylated NHE1 maintained alkaline intracellular pH (pHi) and supported survival of AML cells. Sodium 40-46 glucose-6-phosphate isomerase Homo sapiens 205-208 35624145-7 2022 Plasma from patients taking amiloride for diuresis reduced pHi of leukemia and enhanced cytotoxic effects of kinase inhibitors and chemotherapy in vitro. Amiloride 28-37 glucose-6-phosphate isomerase Homo sapiens 59-62 35603428-1 2022 Glycosylphosphatidylinositols (GPIs) are glycolipids that anchor many proteins (GPI-APs) on the cell surface. Glycosylphosphatidylinositols 31-35 glucose-6-phosphate isomerase Homo sapiens 80-83 35603428-1 2022 Glycosylphosphatidylinositols (GPIs) are glycolipids that anchor many proteins (GPI-APs) on the cell surface. Glycolipids 41-52 glucose-6-phosphate isomerase Homo sapiens 80-83 35603428-2 2022 The core glycan of GPI precursor has three mannoses, which in mammals, are all modified by ethanolamine-phosphate (EthN-P). Polysaccharides 9-15 glucose-6-phosphate isomerase Homo sapiens 19-22 35603428-2 2022 The core glycan of GPI precursor has three mannoses, which in mammals, are all modified by ethanolamine-phosphate (EthN-P). phosphorylethanolamine 91-113 glucose-6-phosphate isomerase Homo sapiens 19-22 35603428-2 2022 The core glycan of GPI precursor has three mannoses, which in mammals, are all modified by ethanolamine-phosphate (EthN-P). phosphorylethanolamine 115-121 glucose-6-phosphate isomerase Homo sapiens 19-22 35603428-3 2022 It is postulated that EthN-P on the third mannose (EthN-P-Man3) is the bridge between GPI and the protein and the second (EthN-P-Man2) is removed after GPI-protein attachment. phosphorylethanolamine 22-28 glucose-6-phosphate isomerase Homo sapiens 86-89 35603428-3 2022 It is postulated that EthN-P on the third mannose (EthN-P-Man3) is the bridge between GPI and the protein and the second (EthN-P-Man2) is removed after GPI-protein attachment. phosphorylethanolamine 22-28 glucose-6-phosphate isomerase Homo sapiens 152-155 35603428-3 2022 It is postulated that EthN-P on the third mannose (EthN-P-Man3) is the bridge between GPI and the protein and the second (EthN-P-Man2) is removed after GPI-protein attachment. Mannose 42-49 glucose-6-phosphate isomerase Homo sapiens 86-89 35603428-3 2022 It is postulated that EthN-P on the third mannose (EthN-P-Man3) is the bridge between GPI and the protein and the second (EthN-P-Man2) is removed after GPI-protein attachment. ethn-p-man3 51-62 glucose-6-phosphate isomerase Homo sapiens 86-89 35603428-3 2022 It is postulated that EthN-P on the third mannose (EthN-P-Man3) is the bridge between GPI and the protein and the second (EthN-P-Man2) is removed after GPI-protein attachment. ethn-p-man2 122-133 glucose-6-phosphate isomerase Homo sapiens 152-155 35603428-5 2022 Here, we show that EthN-P on Man2 is the preferential bridge in some GPI-APs, among them, the Ect-5"-nucleotidase and Netrin G2. phosphorylethanolamine 19-25 glucose-6-phosphate isomerase Homo sapiens 69-72 35603428-5 2022 Here, we show that EthN-P on Man2 is the preferential bridge in some GPI-APs, among them, the Ect-5"-nucleotidase and Netrin G2. man2 29-33 glucose-6-phosphate isomerase Homo sapiens 69-72 35603428-6 2022 We find that CD59, a GPI-AP, is attached via EthN-P-Man2 both in PIGB-knockout cells, in which GPI lacks Man3, and with a small fraction in wild-type cells. ethn-p-man2 45-56 glucose-6-phosphate isomerase Homo sapiens 21-24 35603428-6 2022 We find that CD59, a GPI-AP, is attached via EthN-P-Man2 both in PIGB-knockout cells, in which GPI lacks Man3, and with a small fraction in wild-type cells. ethn-p-man2 45-56 glucose-6-phosphate isomerase Homo sapiens 95-98 35428816-7 2022 Ion and water transporters might participate in the maintenance of pHi and mitochondria homeostasis in differentiated SPs, which may contribute, combined with integral barrier functions, to efficient water efflux. Water 8-13 glucose-6-phosphate isomerase Homo sapiens 67-70 35503765-1 2022 Sodium-hydrogen exchangers (NHEs) tightly regulate intracellular pH (pHi), proliferation, migration and cell volume. Sodium 0-6 glucose-6-phosphate isomerase Homo sapiens 69-72 35503765-1 2022 Sodium-hydrogen exchangers (NHEs) tightly regulate intracellular pH (pHi), proliferation, migration and cell volume. Hydrogen 7-15 glucose-6-phosphate isomerase Homo sapiens 69-72 35428816-7 2022 Ion and water transporters might participate in the maintenance of pHi and mitochondria homeostasis in differentiated SPs, which may contribute, combined with integral barrier functions, to efficient water efflux. Water 200-205 glucose-6-phosphate isomerase Homo sapiens 67-70 35353246-7 2022 Very much and much improvement was reported on the PGI-I by 84% and 85% of participants in the FCM and no-FCM groups respectively. Fosfomycin 95-98 glucose-6-phosphate isomerase Homo sapiens 51-54 35510238-0 2022 PGI score: prospective validation and correlation with SOFA, SAPS-II, and APACHE-II scores for predicting outcomes in acute aluminum phosphide poisoning. aluminum phosphide 124-142 glucose-6-phosphate isomerase Homo sapiens 0-3 35510238-1 2022 Introduction: We recently derived a simplified 3-point PGI score (representing blood pH < 7.25, Glasgow coma scale (GCS) score < 13, and impaired systolic blood pressure (SBP) < 90 mm Hg), which accurately predicted in-hospital case fatality ratio (CFR) in acute aluminum phosphide poisoning. aluminum phosphide 263-281 glucose-6-phosphate isomerase Homo sapiens 55-58 35353246-7 2022 Very much and much improvement was reported on the PGI-I by 84% and 85% of participants in the FCM and no-FCM groups respectively. Fosfomycin 106-109 glucose-6-phosphate isomerase Homo sapiens 51-54 34988781-7 2022 The EID-CT reconstructions were performed using the dedicated calcium score kernel Sa36. Calcium 62-69 glucose-6-phosphate isomerase Homo sapiens 83-87 35049438-13 2022 At the same time, after alpha-solanine treatment, the expression of the energy metabolism pathway-related proteins including GPI, ALDOA, TPI1, PKLR, LDHA and ALDH3 was reduced. alpha-solanine 24-38 glucose-6-phosphate isomerase Homo sapiens 125-128 35057031-7 2022 On the other hand, 2DG lacks the ability to convert into fructose-6-phosphate, resulting in a hampering of the activity of both glucose-6-phosphate isomerase and hexokinase, and finally causing cell death. Deoxyglucose 19-22 glucose-6-phosphate isomerase Homo sapiens 128-157 35057031-7 2022 On the other hand, 2DG lacks the ability to convert into fructose-6-phosphate, resulting in a hampering of the activity of both glucose-6-phosphate isomerase and hexokinase, and finally causing cell death. fructose-6-phosphate 57-77 glucose-6-phosphate isomerase Homo sapiens 128-157 35281309-3 2021 This study followed the accumulation of acid anion into the cell pellet and parallel changes in pHi in two human pathogenic strains of L. monocytogenes (N1-227 and R2-499) and in E. coli O157:H7 after exposure to sub-bacteriostatic levels of lactic and acetic acids at mildly acidic pH 6. lactic 242-248 glucose-6-phosphate isomerase Homo sapiens 96-99 35281309-3 2021 This study followed the accumulation of acid anion into the cell pellet and parallel changes in pHi in two human pathogenic strains of L. monocytogenes (N1-227 and R2-499) and in E. coli O157:H7 after exposure to sub-bacteriostatic levels of lactic and acetic acids at mildly acidic pH 6. Acetates 253-265 glucose-6-phosphate isomerase Homo sapiens 96-99 2555323-11 1989 The removal of external Ca2+i inhibited the ionomycin-induced elevation of both Ca2+i and pHi. Ionomycin 44-53 glucose-6-phosphate isomerase Homo sapiens 90-93 35005560-6 2022 The pHi transients provide dynamic alterations of H+ transport required for ATP synthesis, and a decrease in pHi may serve as a negative feedback to cardiac contractions. Adenosine Triphosphate 76-79 glucose-6-phosphate isomerase Homo sapiens 4-7 24213040-1 1989 Internal pH (pHi) was determined inEmiliania huxleyi (Lohmann) using the probe 2",7"-bis-(2-carboxyethyl)-5(and-6)carboxyfluoresceinacetoxymethylester (BCEF-AM) and digital imaging microscopy. 2",7"-bis-(2-carboxyethyl)-5 79-107 glucose-6-phosphate isomerase Homo sapiens 13-16 24213040-1 1989 Internal pH (pHi) was determined inEmiliania huxleyi (Lohmann) using the probe 2",7"-bis-(2-carboxyethyl)-5(and-6)carboxyfluoresceinacetoxymethylester (BCEF-AM) and digital imaging microscopy. carboxyfluoresceinacetoxymethylester 114-150 glucose-6-phosphate isomerase Homo sapiens 13-16 24213040-3 1989 A linear relationship was established between pHi and the 490/450 fluorescence ratio of BCECF-AM over the pH range 6.0 to 8.0 using the ionophore nigericin. 2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester 88-96 glucose-6-phosphate isomerase Homo sapiens 46-49 24213040-3 1989 A linear relationship was established between pHi and the 490/450 fluorescence ratio of BCECF-AM over the pH range 6.0 to 8.0 using the ionophore nigericin. Nigericin 146-155 glucose-6-phosphate isomerase Homo sapiens 46-49 24213040-7 1989 The average pHi was 7.29 (+-0.11) for cells in the presence of 2 mM HCO 3 (-) . 7 alpha-hydroxy-4-cholesten-3-one 68-71 glucose-6-phosphate isomerase Homo sapiens 12-15 24213040-8 1989 In the absence of HCO 3 (-) the pHi was decreased by 0.8 pH unit. 7 alpha-hydroxy-4-cholesten-3-one 18-21 glucose-6-phosphate isomerase Homo sapiens 32-35 2558106-5 1989 SF, AMF, and MSF are soluble and heat labile proteins with Mr of 77, 55, and 70 kd by SDS-PAGE, respectively, and may be members of a new class of cell-specific regulators of motility. Sodium Dodecyl Sulfate 86-89 glucose-6-phosphate isomerase Homo sapiens 4-7 2555323-7 1989 Inhibition of the thrombin-induced increase in Ca2+i with 8-diethylaminooctyl 3,4,5-trimethoxybenzoate hydrochloride or bis-(o-aminophenoxy)ethane-N,N,N",N"- tetraacetic acid also inhibited the thrombin-stimulated increase in pHi. ca2+i 47-52 glucose-6-phosphate isomerase Homo sapiens 226-229 2555323-7 1989 Inhibition of the thrombin-induced increase in Ca2+i with 8-diethylaminooctyl 3,4,5-trimethoxybenzoate hydrochloride or bis-(o-aminophenoxy)ethane-N,N,N",N"- tetraacetic acid also inhibited the thrombin-stimulated increase in pHi. bis-(o-aminophenoxy)ethane-n,n,n",n"- tetraacetic acid 120-174 glucose-6-phosphate isomerase Homo sapiens 226-229 2555323-9 1989 When Ca2+i was elevated with ionomycin, a concomitant increase in pHi was observed. Ionomycin 29-38 glucose-6-phosphate isomerase Homo sapiens 66-69 2553125-3 1989 The presence of 7% poly(ethylene glycol) increased co-pelleting of the latter four enzymes and two other enzymes, glucose-6-phosphate isomerase, and phosphoglycerate kinase with microtubules. Polyethylene Glycols 19-40 glucose-6-phosphate isomerase Homo sapiens 114-143 2555323-10 1989 The increase in pHi due to ionomycin was dependent on Na+ and sensitive to amiloride. Ionomycin 27-36 glucose-6-phosphate isomerase Homo sapiens 16-19 2555323-10 1989 The increase in pHi due to ionomycin was dependent on Na+ and sensitive to amiloride. Amiloride 75-84 glucose-6-phosphate isomerase Homo sapiens 16-19 2555323-12 1989 The ionomycin-induced increases in Ca2+i and pHi were not inhibited by 8-diethylaminooctyl 3,4,5-trimethoxy-benzoate hydrochloride. Ionomycin 4-13 glucose-6-phosphate isomerase Homo sapiens 45-48 2556941-3 1989 The dependence of the anion exchanger on pHi was studied using microspectrofluorimetry of the pH-sensitive dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 111-158 glucose-6-phosphate isomerase Homo sapiens 41-44 2556941-3 1989 The dependence of the anion exchanger on pHi was studied using microspectrofluorimetry of the pH-sensitive dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 160-165 glucose-6-phosphate isomerase Homo sapiens 41-44 2557452-12 1989 These results indicate that prolonged aldosterone treatment raises pHi and increases gK+ by promoting insertion of K+ channels into the cell membrane. Aldosterone 38-49 glucose-6-phosphate isomerase Homo sapiens 67-70 2790039-1 1989 Differentiation of HL60 cells into neutrophil-like cells after exposure to dimethylsulfoxide is accompanied by an increase in intracellular pH (pHi) which results from an increased activity of the Na+/H+ antiporter at physiological pHi value, but not at acidic pHi values. Dimethyl Sulfoxide 75-92 glucose-6-phosphate isomerase Homo sapiens 144-147 2621616-11 1989 Removal of NH4Cl or addition of sodium acetate (pHo 7.4) reduced pHi but this gave either an increase of tension (papillary muscle) or an initial fall followed by a subsequent recovery of tension (Purkinje fibre). Ammonium Chloride 11-16 glucose-6-phosphate isomerase Homo sapiens 65-68 2621616-11 1989 Removal of NH4Cl or addition of sodium acetate (pHo 7.4) reduced pHi but this gave either an increase of tension (papillary muscle) or an initial fall followed by a subsequent recovery of tension (Purkinje fibre). Sodium Acetate 32-46 glucose-6-phosphate isomerase Homo sapiens 65-68 2551762-2 1989 Intracellular pH (pHi) was measured by using the fluorescent dye 2",7"-bis(carboxyethyl)-5(6)-carboxyfluorescein. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 65-112 glucose-6-phosphate isomerase Homo sapiens 18-21 2506759-2 1989 Cl- removal causes reversal of apical Cl- -HCO3- exchange, resulting in a fall in intracellular Cl- activity (aiCl) and an increase in intracellular pH (pHi). Bicarbonates 43-47 glucose-6-phosphate isomerase Homo sapiens 153-156 2790039-1 1989 Differentiation of HL60 cells into neutrophil-like cells after exposure to dimethylsulfoxide is accompanied by an increase in intracellular pH (pHi) which results from an increased activity of the Na+/H+ antiporter at physiological pHi value, but not at acidic pHi values. Dimethyl Sulfoxide 75-92 glucose-6-phosphate isomerase Homo sapiens 232-235 2790039-1 1989 Differentiation of HL60 cells into neutrophil-like cells after exposure to dimethylsulfoxide is accompanied by an increase in intracellular pH (pHi) which results from an increased activity of the Na+/H+ antiporter at physiological pHi value, but not at acidic pHi values. Dimethyl Sulfoxide 75-92 glucose-6-phosphate isomerase Homo sapiens 232-235 2548005-3 1989 Under in vivo conditions, VZV gpI was phosphorylated on its serine and threonine residues by protein kinases present within lysates of either VZV-infected or uninfected cells. Serine 60-66 glucose-6-phosphate isomerase Homo sapiens 30-33 2505795-1 1989 The role of calcium in cytoplasmic pH (pHi) changes was studied using 2",7"-bis(2-carboxyethyl)-5-(and 6-)carboxyfluorescein, an internalized fluorescent pH indicator, in cultured porcine thyroid cells. Calcium 12-19 glucose-6-phosphate isomerase Homo sapiens 35-37 2505795-1 1989 The role of calcium in cytoplasmic pH (pHi) changes was studied using 2",7"-bis(2-carboxyethyl)-5-(and 6-)carboxyfluorescein, an internalized fluorescent pH indicator, in cultured porcine thyroid cells. Calcium 12-19 glucose-6-phosphate isomerase Homo sapiens 39-42 2505795-1 1989 The role of calcium in cytoplasmic pH (pHi) changes was studied using 2",7"-bis(2-carboxyethyl)-5-(and 6-)carboxyfluorescein, an internalized fluorescent pH indicator, in cultured porcine thyroid cells. Calcium 12-19 glucose-6-phosphate isomerase Homo sapiens 39-41 2694498-3 1989 Two isolates of P. malariae from patients at Kanchanaburi showed a band of GPI activity on cellulose acetate gels at a cathodal position quite distinct from that of any previously known GPI variants in other human malaria parasites. acetylcellulose 91-108 glucose-6-phosphate isomerase Homo sapiens 75-78 2481729-3 1989 In the presence of CO2-HCO3-, the intracellular HCO3- activity (aiHCO3) was estimated from pHi. co2-hco3 19-27 glucose-6-phosphate isomerase Homo sapiens 91-94 2481729-3 1989 In the presence of CO2-HCO3-, the intracellular HCO3- activity (aiHCO3) was estimated from pHi. Bicarbonates 23-27 glucose-6-phosphate isomerase Homo sapiens 91-94 2481729-3 1989 In the presence of CO2-HCO3-, the intracellular HCO3- activity (aiHCO3) was estimated from pHi. aihco3 64-70 glucose-6-phosphate isomerase Homo sapiens 91-94 2481729-20 1989 Simultaneous measurements of EGABA, aiCl and aiHCO3 (pHi) gave a PHCO3/PCl value of 0.33. aihco3 45-51 glucose-6-phosphate isomerase Homo sapiens 53-56 2481729-20 1989 Simultaneous measurements of EGABA, aiCl and aiHCO3 (pHi) gave a PHCO3/PCl value of 0.33. phco3 65-70 glucose-6-phosphate isomerase Homo sapiens 53-56 2576148-1 1989 Hormonal stimulation of Na+/H+ exchange increased intracellular pH (pHi) in a dose-dependent manner in proximal tubules suspended in Krebs-Henseleit buffer (KHB) supplemented with 25 mM HCO3- and CO2 (KHB + HCO3). Krebs-Henseleit solution 133-155 glucose-6-phosphate isomerase Homo sapiens 68-71 2576148-1 1989 Hormonal stimulation of Na+/H+ exchange increased intracellular pH (pHi) in a dose-dependent manner in proximal tubules suspended in Krebs-Henseleit buffer (KHB) supplemented with 25 mM HCO3- and CO2 (KHB + HCO3). Krebs-Henseleit solution 157-160 glucose-6-phosphate isomerase Homo sapiens 68-71 2576148-1 1989 Hormonal stimulation of Na+/H+ exchange increased intracellular pH (pHi) in a dose-dependent manner in proximal tubules suspended in Krebs-Henseleit buffer (KHB) supplemented with 25 mM HCO3- and CO2 (KHB + HCO3). Bicarbonates 186-190 glucose-6-phosphate isomerase Homo sapiens 68-71 2576148-1 1989 Hormonal stimulation of Na+/H+ exchange increased intracellular pH (pHi) in a dose-dependent manner in proximal tubules suspended in Krebs-Henseleit buffer (KHB) supplemented with 25 mM HCO3- and CO2 (KHB + HCO3). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 196-199 glucose-6-phosphate isomerase Homo sapiens 68-71 2576148-2 1989 The maximum increase in pHi was approximately 45% of the response observed with segments suspended in bicarbonate-free buffer (KHB-HCO3) and the time required to achieve maximum pHi alterations was significantly increased (p less than 0.05) in the presence of KHB + HCO3 when compared to responses obtained in KHB - HCO3. Bicarbonates 102-113 glucose-6-phosphate isomerase Homo sapiens 24-27 2576148-2 1989 The maximum increase in pHi was approximately 45% of the response observed with segments suspended in bicarbonate-free buffer (KHB-HCO3) and the time required to achieve maximum pHi alterations was significantly increased (p less than 0.05) in the presence of KHB + HCO3 when compared to responses obtained in KHB - HCO3. khb-hco3 127-135 glucose-6-phosphate isomerase Homo sapiens 24-27 2576148-2 1989 The maximum increase in pHi was approximately 45% of the response observed with segments suspended in bicarbonate-free buffer (KHB-HCO3) and the time required to achieve maximum pHi alterations was significantly increased (p less than 0.05) in the presence of KHB + HCO3 when compared to responses obtained in KHB - HCO3. Krebs-Henseleit solution 127-130 glucose-6-phosphate isomerase Homo sapiens 24-27 2576148-2 1989 The maximum increase in pHi was approximately 45% of the response observed with segments suspended in bicarbonate-free buffer (KHB-HCO3) and the time required to achieve maximum pHi alterations was significantly increased (p less than 0.05) in the presence of KHB + HCO3 when compared to responses obtained in KHB - HCO3. Bicarbonates 131-135 glucose-6-phosphate isomerase Homo sapiens 24-27 2576148-2 1989 The maximum increase in pHi was approximately 45% of the response observed with segments suspended in bicarbonate-free buffer (KHB-HCO3) and the time required to achieve maximum pHi alterations was significantly increased (p less than 0.05) in the presence of KHB + HCO3 when compared to responses obtained in KHB - HCO3. Krebs-Henseleit solution 260-263 glucose-6-phosphate isomerase Homo sapiens 24-27 2576148-2 1989 The maximum increase in pHi was approximately 45% of the response observed with segments suspended in bicarbonate-free buffer (KHB-HCO3) and the time required to achieve maximum pHi alterations was significantly increased (p less than 0.05) in the presence of KHB + HCO3 when compared to responses obtained in KHB - HCO3. Bicarbonates 266-270 glucose-6-phosphate isomerase Homo sapiens 24-27 2576148-3 1989 Dose-response curves for agonist-induced pHi increases were shifted to the right by a factor of 10 for segments suspended in KHB + HCO3. Krebs-Henseleit solution 125-128 glucose-6-phosphate isomerase Homo sapiens 41-44 2576148-3 1989 Dose-response curves for agonist-induced pHi increases were shifted to the right by a factor of 10 for segments suspended in KHB + HCO3. Bicarbonates 131-135 glucose-6-phosphate isomerase Homo sapiens 41-44 2576148-4 1989 Increases in pHi induced by agonists in KHB + HCO3 were effectively blocked by pretreatment with 10 microM ethylisopropyl amiloride but not with the Cl-/HCO3- inhibitor, DIDS (0.1 mM, 30 min). Krebs-Henseleit solution 40-43 glucose-6-phosphate isomerase Homo sapiens 13-16 2576148-4 1989 Increases in pHi induced by agonists in KHB + HCO3 were effectively blocked by pretreatment with 10 microM ethylisopropyl amiloride but not with the Cl-/HCO3- inhibitor, DIDS (0.1 mM, 30 min). Bicarbonates 46-50 glucose-6-phosphate isomerase Homo sapiens 13-16 2576148-4 1989 Increases in pHi induced by agonists in KHB + HCO3 were effectively blocked by pretreatment with 10 microM ethylisopropyl amiloride but not with the Cl-/HCO3- inhibitor, DIDS (0.1 mM, 30 min). ethylisopropylamiloride 107-131 glucose-6-phosphate isomerase Homo sapiens 13-16 2576148-4 1989 Increases in pHi induced by agonists in KHB + HCO3 were effectively blocked by pretreatment with 10 microM ethylisopropyl amiloride but not with the Cl-/HCO3- inhibitor, DIDS (0.1 mM, 30 min). Bicarbonates 153-157 glucose-6-phosphate isomerase Homo sapiens 13-16 2548005-3 1989 Under in vivo conditions, VZV gpI was phosphorylated on its serine and threonine residues by protein kinases present within lysates of either VZV-infected or uninfected cells. Threonine 71-80 glucose-6-phosphate isomerase Homo sapiens 30-33 2548005-4 1989 Because this activity was diminished by heparin, a known inhibitor of casein kinase II, isolated gpI was incubated with purified casein kinase II and shown to be phosphorylated in an in vitro assay containing [gamma-32P]ATP. Heparin 40-47 glucose-6-phosphate isomerase Homo sapiens 97-100 2548005-4 1989 Because this activity was diminished by heparin, a known inhibitor of casein kinase II, isolated gpI was incubated with purified casein kinase II and shown to be phosphorylated in an in vitro assay containing [gamma-32P]ATP. [gamma-32p 209-219 glucose-6-phosphate isomerase Homo sapiens 97-100 2548005-9 1989 In summary, this study showed that VZV gpI was phosphorylated by each of two mammalian protein kinases (casein kinase I and casein kinase II) and that potential serine-threonine phosphorylation sites for each of these two kinases were present in the viral glycoprotein. Serine 161-167 glucose-6-phosphate isomerase Homo sapiens 39-42 2548005-9 1989 In summary, this study showed that VZV gpI was phosphorylated by each of two mammalian protein kinases (casein kinase I and casein kinase II) and that potential serine-threonine phosphorylation sites for each of these two kinases were present in the viral glycoprotein. Threonine 168-177 glucose-6-phosphate isomerase Homo sapiens 39-42 2546796-0 1989 Potentiation by adrenaline of thrombin-induced elevation of pHi is not essential for synergistic activation of human platelets. Epinephrine 16-26 glucose-6-phosphate isomerase Homo sapiens 60-63 2546796-2 1989 Adrenaline markedly enhanced the thrombin-induced increase in cytoplasmic pH (pHi) in BCECF-loaded platelets. Epinephrine 0-10 glucose-6-phosphate isomerase Homo sapiens 78-81 2546796-2 1989 Adrenaline markedly enhanced the thrombin-induced increase in cytoplasmic pH (pHi) in BCECF-loaded platelets. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 86-91 glucose-6-phosphate isomerase Homo sapiens 78-81 2526587-4 1989 Through the use of the pH-sensitive fluorescent dye, 2,7-biscarboxyethyl-5(6)-carboxy-fluorescein (BCECF), base-line pHi was estimated to be 7.24 +/- 0.03. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 53-97 glucose-6-phosphate isomerase Homo sapiens 117-120 2526587-4 1989 Through the use of the pH-sensitive fluorescent dye, 2,7-biscarboxyethyl-5(6)-carboxy-fluorescein (BCECF), base-line pHi was estimated to be 7.24 +/- 0.03. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 99-104 glucose-6-phosphate isomerase Homo sapiens 117-120 2526587-8 1989 In contrast, N-ethylmaleimide (NEM) and N,N"-dicyclohexylcarbodiimide, nonspecific inhibitors of proton adenosinetriphosphatases (ATPases), virtually abolished pHi recovery. Ethylmaleimide 13-29 glucose-6-phosphate isomerase Homo sapiens 160-163 2526587-8 1989 In contrast, N-ethylmaleimide (NEM) and N,N"-dicyclohexylcarbodiimide, nonspecific inhibitors of proton adenosinetriphosphatases (ATPases), virtually abolished pHi recovery. Dicyclohexylcarbodiimide 40-69 glucose-6-phosphate isomerase Homo sapiens 160-163 2547642-1 1989 Intracellular pH (pHi) was measured in basal corneal epithelial cells from fresh corneal explants using the pH sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein 137-186 glucose-6-phosphate isomerase Homo sapiens 18-21 2743336-6 1989 Addition of CCCP led to a decrease in steady-state pHi values as compared to untreated cells at pHc less than 6.50, whereas there was no apparent effect of CCCP on steady-state pHi values at pHc greater than 6.50. carbonyl 3-chlorophenylhydrazone 12-16 glucose-6-phosphate isomerase Homo sapiens 51-54 2743336-7 1989 The CCCP-induced reduction in steady-state pHi combined with the uncoupling of oxidative phosphorylation by CCCP appeared to be the major mechanisms leading to cell death at pHc less than 6.50. carbonyl 3-chlorophenylhydrazone 4-8 glucose-6-phosphate isomerase Homo sapiens 43-46 2743336-8 1989 The toxicity of CCCP under acidic conditions was enhanced by amiloride and 4,4"-diisothiocyanostilbene-2,2-disulfonic acid, agents which are known to inhibit membrane-based ion exchange mechanisms which regulate pHi under acidic conditions. carbonyl 3-chlorophenylhydrazone 16-20 glucose-6-phosphate isomerase Homo sapiens 212-215 2743336-8 1989 The toxicity of CCCP under acidic conditions was enhanced by amiloride and 4,4"-diisothiocyanostilbene-2,2-disulfonic acid, agents which are known to inhibit membrane-based ion exchange mechanisms which regulate pHi under acidic conditions. Amiloride 61-70 glucose-6-phosphate isomerase Homo sapiens 212-215 2743336-8 1989 The toxicity of CCCP under acidic conditions was enhanced by amiloride and 4,4"-diisothiocyanostilbene-2,2-disulfonic acid, agents which are known to inhibit membrane-based ion exchange mechanisms which regulate pHi under acidic conditions. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 75-122 glucose-6-phosphate isomerase Homo sapiens 212-215 2547642-6 1989 Buffering capacity measured by NH4Cl treatment was 31 mM pH at pHi 7.34 and increased with decreasing pHi. Ammonium Chloride 31-36 glucose-6-phosphate isomerase Homo sapiens 63-66 2547642-6 1989 Buffering capacity measured by NH4Cl treatment was 31 mM pH at pHi 7.34 and increased with decreasing pHi. Ammonium Chloride 31-36 glucose-6-phosphate isomerase Homo sapiens 102-105 2547642-11 1989 Net proton efflux via Na:H exchange increased with decreasing pHi and was enhanced by depleting cells of Nai, suggesting roles for both pHi and Nai in control of Na:H activation. Sodium Iodide 105-108 glucose-6-phosphate isomerase Homo sapiens 136-139 2548914-1 1989 Sodium-dependent intracellular pH (pHi) regulation was compared in granulosa cells from the three largest avian ovarian follicles by monitoring the pHi with biscarboxyethylcarboxyfluorescein, a dye whose fluorescence increases with alkalinity. Sodium 0-6 glucose-6-phosphate isomerase Homo sapiens 35-38 2548914-1 1989 Sodium-dependent intracellular pH (pHi) regulation was compared in granulosa cells from the three largest avian ovarian follicles by monitoring the pHi with biscarboxyethylcarboxyfluorescein, a dye whose fluorescence increases with alkalinity. Sodium 0-6 glucose-6-phosphate isomerase Homo sapiens 148-151 2548914-3 1989 The resting pHi measured in nominally bicarbonate free buffer with extra-cellular Na+ (Nao+ = 144 mM) and external pH (pHo) of 7.3 was about 6.8 in cells from F1, F2, and F3. Bicarbonates 38-49 glucose-6-phosphate isomerase Homo sapiens 12-15 2548914-3 1989 The resting pHi measured in nominally bicarbonate free buffer with extra-cellular Na+ (Nao+ = 144 mM) and external pH (pHo) of 7.3 was about 6.8 in cells from F1, F2, and F3. 10-N-nonylacridinium orange 87-90 glucose-6-phosphate isomerase Homo sapiens 12-15 2548914-5 1989 After acute cytoplasmic acidification by exposure of cells to nigericin in choline+ buffer, or by the abrupt removal of ammonium chloride, complete recovery of pHi occurred in 4-5 min. Nigericin 62-71 glucose-6-phosphate isomerase Homo sapiens 160-163 2548914-5 1989 After acute cytoplasmic acidification by exposure of cells to nigericin in choline+ buffer, or by the abrupt removal of ammonium chloride, complete recovery of pHi occurred in 4-5 min. Ammonium Chloride 120-137 glucose-6-phosphate isomerase Homo sapiens 160-163 2548914-10 1989 In addition, amiloride inhibited the Nao+ dependency of pHi recovery to a similar degree in F1, F2, and F3 cells. Amiloride 13-22 glucose-6-phosphate isomerase Homo sapiens 56-59 2548914-10 1989 In addition, amiloride inhibited the Nao+ dependency of pHi recovery to a similar degree in F1, F2, and F3 cells. 10-N-nonylacridinium orange 37-41 glucose-6-phosphate isomerase Homo sapiens 56-59 2548914-11 1989 Our observations demonstrate in avian granulosa cells the existence of a Nao+-dependent, amiloride-sensitive pHi regulatory system that is equally effective in cells obtained from the three largest yolk-filled follicles. 10-N-nonylacridinium orange 73-77 glucose-6-phosphate isomerase Homo sapiens 109-112 2548914-11 1989 Our observations demonstrate in avian granulosa cells the existence of a Nao+-dependent, amiloride-sensitive pHi regulatory system that is equally effective in cells obtained from the three largest yolk-filled follicles. Amiloride 89-98 glucose-6-phosphate isomerase Homo sapiens 109-112 2543453-1 1989 We studied the effects of epidermal growth factor (EGF), thyroid-stimulating hormone (TSH) and amiloride on cytoplasmic pH (pHi) in cultured porcine thyroid cells. Amiloride 95-104 glucose-6-phosphate isomerase Homo sapiens 120-122 2543453-1 1989 We studied the effects of epidermal growth factor (EGF), thyroid-stimulating hormone (TSH) and amiloride on cytoplasmic pH (pHi) in cultured porcine thyroid cells. Amiloride 95-104 glucose-6-phosphate isomerase Homo sapiens 124-127 2543587-1 1989 Incubation of toad lenses with the acetoxymethyl ester of 2",7"-biscarboxyethyl-5(6)-carboxy-fluorescein led to a highly selective accumulation of the de-esterified, pH-sensitive form of the dye in the epithelial cells, enabling the continuous fluorometric monitoring of epithelial intracellular pH (pHi) in intact lenses. acetoxymethyl ester 35-54 glucose-6-phosphate isomerase Homo sapiens 300-303 2543587-1 1989 Incubation of toad lenses with the acetoxymethyl ester of 2",7"-biscarboxyethyl-5(6)-carboxy-fluorescein led to a highly selective accumulation of the de-esterified, pH-sensitive form of the dye in the epithelial cells, enabling the continuous fluorometric monitoring of epithelial intracellular pH (pHi) in intact lenses. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 58-104 glucose-6-phosphate isomerase Homo sapiens 300-303 2543587-3 1989 Exposure of lenses to hypertonic conditions (by the addition of sucrose to the medium) resulted in a biphasic change in pHi; a rapid initial, "spike-like" decrease was immediately followed by a persistent reversal that raised pHi in CO2/HCO3- -rich and -free media by 0.13 and 0.18 units, respectively. Sucrose 64-71 glucose-6-phosphate isomerase Homo sapiens 120-123 2543587-3 1989 Exposure of lenses to hypertonic conditions (by the addition of sucrose to the medium) resulted in a biphasic change in pHi; a rapid initial, "spike-like" decrease was immediately followed by a persistent reversal that raised pHi in CO2/HCO3- -rich and -free media by 0.13 and 0.18 units, respectively. Sucrose 64-71 glucose-6-phosphate isomerase Homo sapiens 226-229 2543587-3 1989 Exposure of lenses to hypertonic conditions (by the addition of sucrose to the medium) resulted in a biphasic change in pHi; a rapid initial, "spike-like" decrease was immediately followed by a persistent reversal that raised pHi in CO2/HCO3- -rich and -free media by 0.13 and 0.18 units, respectively. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 233-236 glucose-6-phosphate isomerase Homo sapiens 226-229 2543453-2 1989 We used 2",7"-bis(2-carboxyethyl)-5- (and 6-)carboxyfluorescein (BCECF), an internalized fluorescent pH indicator, to measure pHi. 2",7"-bis(2-carboxyethyl)-5- (and 6-)carboxyfluorescein 8-63 glucose-6-phosphate isomerase Homo sapiens 101-103 2543587-4 1989 Under CO2/HCO3- -free conditions, the hypertonic exposure raised pHi to a value near the calculated equilibrium position for a lens Na+/H+ exchanger. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 6-9 glucose-6-phosphate isomerase Homo sapiens 65-68 2543587-4 1989 Under CO2/HCO3- -free conditions, the hypertonic exposure raised pHi to a value near the calculated equilibrium position for a lens Na+/H+ exchanger. Bicarbonates 10-14 glucose-6-phosphate isomerase Homo sapiens 65-68 2543453-2 1989 We used 2",7"-bis(2-carboxyethyl)-5- (and 6-)carboxyfluorescein (BCECF), an internalized fluorescent pH indicator, to measure pHi. 2",7"-bis(2-carboxyethyl)-5- (and 6-)carboxyfluorescein 8-63 glucose-6-phosphate isomerase Homo sapiens 126-129 2543587-6 1989 In contrast, in the presence of 1 mM amiloride or in the absence of extralenticular Na+, sucrose addition induced only a persistent pHi decrease, which could be reversed (in the "amiloride" case) by monensin addition. Sucrose 89-96 glucose-6-phosphate isomerase Homo sapiens 132-135 2543453-2 1989 We used 2",7"-bis(2-carboxyethyl)-5- (and 6-)carboxyfluorescein (BCECF), an internalized fluorescent pH indicator, to measure pHi. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 65-70 glucose-6-phosphate isomerase Homo sapiens 101-103 2723764-9 1989 After stimulation or SD, glia repolarized and pHi became more acidic than at resting levels. SD 0006 21-23 glucose-6-phosphate isomerase Homo sapiens 46-49 2545050-6 1989 The ammonium prepulse technique (25 mM NH4Cl) dropped the pHi to pH 6.80. Ammonium Compounds 4-12 glucose-6-phosphate isomerase Homo sapiens 58-61 2545050-6 1989 The ammonium prepulse technique (25 mM NH4Cl) dropped the pHi to pH 6.80. Ammonium Chloride 39-44 glucose-6-phosphate isomerase Homo sapiens 58-61 2545050-7 1989 A rapid recovery of the pHi after this acidification was observed in NaCl Ringer solution. Sodium Chloride 69-73 glucose-6-phosphate isomerase Homo sapiens 24-27 2545050-11 1989 We conclude that in HEPES buffered solutions human lymphocytes recover pHi via a mechanism dependent on extracellular Na+ and largely accomplished by an amiloride inhibitory Na+/H+ exchanger. HEPES 20-25 glucose-6-phosphate isomerase Homo sapiens 71-74 2493743-2 1989 Intracellular pH (pHi) was measured using the fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescin (BCECF). 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescin 62-110 glucose-6-phosphate isomerase Homo sapiens 18-21 2720408-3 1989 Na+/H+ exchange, a mechanism involved in the regulation of intracellular pH (pHi), is activated by low intracellular pH, is dependent on extracellular Na+, and is inhibited by low extracellular pH (pH less than 6) or by amiloride. Amiloride 220-229 glucose-6-phosphate isomerase Homo sapiens 77-80 2720408-6 1989 pHi was monitored fluorimetrically using the intracellularly trapped pH-sensitive dye bis(carboxyethyl)carboxyfluorescein. bis(carboxyethyl)carboxyfluorescein 86-121 glucose-6-phosphate isomerase Homo sapiens 0-3 2720408-10 1989 This pHi reversal following lactic acid-induced acidification was also inhibited at pHo less than 6. Lactic Acid 28-39 glucose-6-phosphate isomerase Homo sapiens 5-8 2493743-2 1989 Intracellular pH (pHi) was measured using the fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescin (BCECF). bcecf 112-117 glucose-6-phosphate isomerase Homo sapiens 18-21 2541250-3 1989 Red blood cells with different cell Na+ (Nai) and pH (pHi) were prepared by nystatin and DIDS treatment of acid-loaded cells. Nystatin 76-84 glucose-6-phosphate isomerase Homo sapiens 54-57 2742613-4 1989 The blocking activity in vitro (GPI and MVD tests) to the mu- and delta-receptors depends on cycle size, localization of disulphide bridge in the cycle, and amino acid configuration at second and fifth positions. disulphide 121-131 glucose-6-phosphate isomerase Homo sapiens 32-35 2541250-3 1989 Red blood cells with different cell Na+ (Nai) and pH (pHi) were prepared by nystatin and DIDS treatment of acid-loaded cells. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 89-93 glucose-6-phosphate isomerase Homo sapiens 54-57 2541250-5 1989 Net Na+ influx (Nai less than 2.0 mmol/liter cell, Nao = 150 mM) increased sigmoidally (Hill coefficient 2.5) when pHi fell below 7.0 and the external pHo was 8.0, but increased linearly at pHo 6.0. Sodium Iodide 16-19 glucose-6-phosphate isomerase Homo sapiens 115-118 2585301-3 1989 We then calculated the intracellular buffering power (beta i) from the pHi changes associated with the influx or efflux of a variety of weak acids or bases. weak acids 136-146 glucose-6-phosphate isomerase Homo sapiens 71-74 2539095-6 1989 The site of the metabolism controlled by the pHi change seemed to be the 5-lipoxygenase, because the production of all the metabolites decreased in parallel and the release of [3H]arachidonic acid from the neutrophils in response to the ionophore was not affected by the pHi change. [3h]arachidonic acid 176-196 glucose-6-phosphate isomerase Homo sapiens 45-48 2536415-1 1989 In various mammalian cell types the stimulation of the plasma membrane amiloride-sensitive Na+/H+ exchange and the resulting increase of intracellular pH (pHi) play a key role in the initiation of cell proliferation. Amiloride 71-80 glucose-6-phosphate isomerase Homo sapiens 155-158 2555474-3 1989 The influence of externally applied weak acids and bases on the total intracellular buffering power (beta T) was investigated by monitoring the pHi changes caused by the intracellular ionophoretic injection of HCl. weak acids 36-46 glucose-6-phosphate isomerase Homo sapiens 144-147 2555474-5 1989 In the absence of weak acids or bases a reduction in the extracellular HEPES concentration had no effect on pHi or on beta T. It did, however, reduce slightly the rate of pHi recovery following HCl injection. Hydrochloric Acid 194-197 glucose-6-phosphate isomerase Homo sapiens 171-174 2555474-7 1989 The presence of CO2 greatly increased beta T. However, as predicted for an open buffer system, the contributions to intracellular buffering by CO2 (beta CO2) decreased as pHi decreased. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 16-19 glucose-6-phosphate isomerase Homo sapiens 171-174 2555474-7 1989 The presence of CO2 greatly increased beta T. However, as predicted for an open buffer system, the contributions to intracellular buffering by CO2 (beta CO2) decreased as pHi decreased. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 143-146 glucose-6-phosphate isomerase Homo sapiens 171-174 2555474-7 1989 The presence of CO2 greatly increased beta T. However, as predicted for an open buffer system, the contributions to intracellular buffering by CO2 (beta CO2) decreased as pHi decreased. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 143-146 glucose-6-phosphate isomerase Homo sapiens 171-174 2555474-9 1989 When added to the superfusate, procaine, 4-aminopyridine, trimethylamine and NH4Cl (1-10 mM) all increased steady-state pHi. Procaine 31-39 glucose-6-phosphate isomerase Homo sapiens 120-123 2555474-9 1989 When added to the superfusate, procaine, 4-aminopyridine, trimethylamine and NH4Cl (1-10 mM) all increased steady-state pHi. 4-Aminopyridine 41-56 glucose-6-phosphate isomerase Homo sapiens 120-123 2555474-9 1989 When added to the superfusate, procaine, 4-aminopyridine, trimethylamine and NH4Cl (1-10 mM) all increased steady-state pHi. trimethylamine 58-72 glucose-6-phosphate isomerase Homo sapiens 120-123 2555474-9 1989 When added to the superfusate, procaine, 4-aminopyridine, trimethylamine and NH4Cl (1-10 mM) all increased steady-state pHi. Ammonium Chloride 77-82 glucose-6-phosphate isomerase Homo sapiens 120-123 2493002-11 1989 In addition to abolishing the mitogen-induced alkalinization, bicarbonate stabilizes pHi at 7.25 units as the external pH (pHe) is varied from 7.0 to 7.6. Bicarbonates 62-73 glucose-6-phosphate isomerase Homo sapiens 85-88 2493002-12 1989 In contrast, in a bicarbonate-free medium pHi increases from 6.9 to 7.3 over the same range of external pHs. Bicarbonates 18-29 glucose-6-phosphate isomerase Homo sapiens 42-45 2493002-13 1989 At a constant external pH, increasing the bicarbonate/CO2 concentration results in an increase in pHi from 6.9 in bicarbonate-free solution to 7.25 in a bicarbonate-buffered medium. Bicarbonates 42-53 glucose-6-phosphate isomerase Homo sapiens 98-101 2493002-13 1989 At a constant external pH, increasing the bicarbonate/CO2 concentration results in an increase in pHi from 6.9 in bicarbonate-free solution to 7.25 in a bicarbonate-buffered medium. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 54-57 glucose-6-phosphate isomerase Homo sapiens 98-101 2493002-13 1989 At a constant external pH, increasing the bicarbonate/CO2 concentration results in an increase in pHi from 6.9 in bicarbonate-free solution to 7.25 in a bicarbonate-buffered medium. Bicarbonates 114-125 glucose-6-phosphate isomerase Homo sapiens 98-101 2493002-13 1989 At a constant external pH, increasing the bicarbonate/CO2 concentration results in an increase in pHi from 6.9 in bicarbonate-free solution to 7.25 in a bicarbonate-buffered medium. Bicarbonates 114-125 glucose-6-phosphate isomerase Homo sapiens 98-101 2918752-1 1989 A method is presented for measuring intracellular pH (pHi) spectrophotometrically in hippocampal slices using the pH dye indicator, Neutral red (NR). Neutral Red 132-143 glucose-6-phosphate isomerase Homo sapiens 54-57 2555474-10 1989 Procaine was fastest at increasing pHi and 4-aminopyridine the slowest. Procaine 0-8 glucose-6-phosphate isomerase Homo sapiens 35-38 2555474-13 1989 HCl injection in the presence of procaine usually resulted in steady-state pHi changes with no pHi transients. Hydrochloric Acid 0-3 glucose-6-phosphate isomerase Homo sapiens 75-78 2585301-7 1989 Although solutions were nominally CO2-free, blockage of pHi regulation with SITS (4-acetamido-4"-isothiocyanatostilbene-2,2"-disulphonic acid) increased the sizes of the pHi changes upon weak acid addition and removal. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 34-37 glucose-6-phosphate isomerase Homo sapiens 56-59 2555474-13 1989 HCl injection in the presence of procaine usually resulted in steady-state pHi changes with no pHi transients. Procaine 33-41 glucose-6-phosphate isomerase Homo sapiens 75-78 2555474-14 1989 In the presence of the other three weak bases HCl injections resulted in intracellular acidifications which were followed by pHi recovery-like transients. Hydrochloric Acid 46-49 glucose-6-phosphate isomerase Homo sapiens 125-128 2585301-7 1989 Although solutions were nominally CO2-free, blockage of pHi regulation with SITS (4-acetamido-4"-isothiocyanatostilbene-2,2"-disulphonic acid) increased the sizes of the pHi changes upon weak acid addition and removal. 4-acetamido-4"-isothiocyanatostilbene-2,2"-disulphonic acid 82-141 glucose-6-phosphate isomerase Homo sapiens 56-59 2555474-20 1989 I found a clear relationship between the concentration of external buffer (HEPES) and the rate at which weak bases, applied to the superfusate, were able to increase pHi. HEPES 75-80 glucose-6-phosphate isomerase Homo sapiens 166-169 2585301-7 1989 Although solutions were nominally CO2-free, blockage of pHi regulation with SITS (4-acetamido-4"-isothiocyanatostilbene-2,2"-disulphonic acid) increased the sizes of the pHi changes upon weak acid addition and removal. 4-acetamido-4"-isothiocyanatostilbene-2,2"-disulphonic acid 82-141 glucose-6-phosphate isomerase Homo sapiens 170-173 2585301-13 1989 Removing the influence of pHi regulation on the undershoots after NH4Cl removal was found to decrease the apparent measured values of beta i by 10%. Ammonium Chloride 66-71 glucose-6-phosphate isomerase Homo sapiens 26-29 2650613-4 1989 Intracellular pH (pHi) was increased by TPA treatment; however, while the alkalinization of K-562 cells was dependent on the presence of extracellular Na+, the response of U-937 cells was unaffected by the removal of this cation. Tetradecanoylphorbol Acetate 40-43 glucose-6-phosphate isomerase Homo sapiens 18-21 2650613-5 1989 In each cell type the protein kinase C (PKC) inhibitor H-7 largely attenuated the TPA induced increase of pHi. 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine 55-58 glucose-6-phosphate isomerase Homo sapiens 106-109 2650613-5 1989 In each cell type the protein kinase C (PKC) inhibitor H-7 largely attenuated the TPA induced increase of pHi. Tetradecanoylphorbol Acetate 82-85 glucose-6-phosphate isomerase Homo sapiens 106-109 2778730-9 1989 CCCP produced an initial alkalosis of less than 0.1 units and thereafter a fall in pHi of 0.4-0.6 units during which the sarcolemmal H+ driving force decreased from 61 to 15 mV. Carbonyl Cyanide m-Chlorophenyl Hydrazone 0-4 glucose-6-phosphate isomerase Homo sapiens 83-86 2612439-2 1989 The pHi values were obtained from the chemical shift differences between the phosphoarginine PA resonance and the inorganic phosphate Pi resonance. phospho-L-arginine 77-92 glucose-6-phosphate isomerase Homo sapiens 4-7 2612439-2 1989 The pHi values were obtained from the chemical shift differences between the phosphoarginine PA resonance and the inorganic phosphate Pi resonance. Protactinium 93-95 glucose-6-phosphate isomerase Homo sapiens 4-7 2612439-2 1989 The pHi values were obtained from the chemical shift differences between the phosphoarginine PA resonance and the inorganic phosphate Pi resonance. Phosphates 124-133 glucose-6-phosphate isomerase Homo sapiens 4-7 2915212-2 1989 Intracellular pH (pHi) of the squid axon is regulated by a stilbenesensitive transporter that couples the influx of Na+ and HCO3- (or the equivalent) to the efflux of Cl-. Bicarbonates 124-128 glucose-6-phosphate isomerase Homo sapiens 18-21 2915212-7 1989 In the presence of both external Na+ and HCO3- (pHo = 8.0, 22 degrees C), pHi increased due to the pHi-regulating mechanism. Bicarbonates 41-45 glucose-6-phosphate isomerase Homo sapiens 74-77 2915212-7 1989 In the presence of both external Na+ and HCO3- (pHo = 8.0, 22 degrees C), pHi increased due to the pHi-regulating mechanism. Bicarbonates 41-45 glucose-6-phosphate isomerase Homo sapiens 99-102 2535694-1 1989 Besides its effect in inhibiting proliferation and inducing differentiation of HL-60 to monocyte-like cells, 1,25-dihydroxyvitamin D3 also causes a rise in intracellular pH (pHi) from 7.17 +/- 0.02 to 7.3 +/- 0.05, as measured by the fluorescence of 2",7"-bis(carboxyethyl)-5-(6)-carboxy-fluorescein. Calcitriol 109-133 glucose-6-phosphate isomerase Homo sapiens 174-177 2535694-1 1989 Besides its effect in inhibiting proliferation and inducing differentiation of HL-60 to monocyte-like cells, 1,25-dihydroxyvitamin D3 also causes a rise in intracellular pH (pHi) from 7.17 +/- 0.02 to 7.3 +/- 0.05, as measured by the fluorescence of 2",7"-bis(carboxyethyl)-5-(6)-carboxy-fluorescein. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 250-299 glucose-6-phosphate isomerase Homo sapiens 174-177 2535694-2 1989 The effect of 1,25-dihydroxyvitamin D3 on pHi is dose dependent in a parallel manner to its effect on the proliferation and differentiation processes. Calcitriol 14-38 glucose-6-phosphate isomerase Homo sapiens 42-45 2535694-3 1989 The elevation of pHi by 1,25-dihydroxyvitamin D3 is gradual but precedes the differentiation process. Calcitriol 24-48 glucose-6-phosphate isomerase Homo sapiens 17-20 2535694-6 1989 In contrast, other agents such as phorbol 12-myristate 13-acetate known to induce differentiation in this cell line do cause an increase in pHi. Tetradecanoylphorbol Acetate 34-65 glucose-6-phosphate isomerase Homo sapiens 140-143 2778730-20 1989 It is concluded that CCCP induces a sarcolemmal H+ conductance which leads to a fall in pHi and to a depolarization of the membrane potential. Carbonyl Cyanide m-Chlorophenyl Hydrazone 21-25 glucose-6-phosphate isomerase Homo sapiens 88-91 2699758-3 1989 Vasoactive intestinal polypeptide (VIP) and peptide with N- and C-terminal histidine (PHI) are released together with acetylcholine from parasympathetic nerves. Histidine 75-84 glucose-6-phosphate isomerase Homo sapiens 86-89 2542998-2 1989 Indeed, part of the contraction of the GPI in response to neurokinins appears to be mediated by acetylcholine and possibly prostaglandins. Acetylcholine 96-109 glucose-6-phosphate isomerase Homo sapiens 39-42 2542998-2 1989 Indeed, part of the contraction of the GPI in response to neurokinins appears to be mediated by acetylcholine and possibly prostaglandins. Prostaglandins 123-137 glucose-6-phosphate isomerase Homo sapiens 39-42 3191476-2 1988 The identity of three PHI variants in the purified preparation could be demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing analysis. Sodium Dodecyl Sulfate 88-110 glucose-6-phosphate isomerase Homo sapiens 22-25 3191476-2 1988 The identity of three PHI variants in the purified preparation could be demonstrated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and isoelectric focusing analysis. polyacrylamide gels 111-129 glucose-6-phosphate isomerase Homo sapiens 22-25 2849306-3 1988 We have developed a technique to measure the fluorescence of a pH-sensitive dye (2,7-biscarboxyethyl-5(6)-carboxyfluorescein) in single glomerular mesangial cells in culture. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 81-124 glucose-6-phosphate isomerase Homo sapiens 63-65 2848021-5 1988 Agents such as forskolin, 8-Br-cAMP, and isoproterenol which raise intracellular cAMP levels inhibit the HCO3-/Cl- antiporter by shifting its pHi dependence in the alkaline direction. Colforsin 15-24 glucose-6-phosphate isomerase Homo sapiens 142-145 2848021-5 1988 Agents such as forskolin, 8-Br-cAMP, and isoproterenol which raise intracellular cAMP levels inhibit the HCO3-/Cl- antiporter by shifting its pHi dependence in the alkaline direction. 8-Bromo Cyclic Adenosine Monophosphate 26-35 glucose-6-phosphate isomerase Homo sapiens 142-145 2848021-5 1988 Agents such as forskolin, 8-Br-cAMP, and isoproterenol which raise intracellular cAMP levels inhibit the HCO3-/Cl- antiporter by shifting its pHi dependence in the alkaline direction. Isoproterenol 41-54 glucose-6-phosphate isomerase Homo sapiens 142-145 2848021-5 1988 Agents such as forskolin, 8-Br-cAMP, and isoproterenol which raise intracellular cAMP levels inhibit the HCO3-/Cl- antiporter by shifting its pHi dependence in the alkaline direction. Cyclic AMP 31-35 glucose-6-phosphate isomerase Homo sapiens 142-145 2848021-5 1988 Agents such as forskolin, 8-Br-cAMP, and isoproterenol which raise intracellular cAMP levels inhibit the HCO3-/Cl- antiporter by shifting its pHi dependence in the alkaline direction. Bicarbonates 105-109 glucose-6-phosphate isomerase Homo sapiens 142-145 2848021-9 1988 Bicarbonate turns on the HCO3-/Cl- antiporter, decreases pHi and allows its regulation by protein kinase C through the Na+/H+ antiporter. Bicarbonates 0-11 glucose-6-phosphate isomerase Homo sapiens 57-60 2848021-10 1988 Inhibition of the HCO3-/Cl- antiporter by cAMP increases the pHi and switches off the protein kinase C-mediated regulation. Bicarbonates 18-22 glucose-6-phosphate isomerase Homo sapiens 61-64 2848021-10 1988 Inhibition of the HCO3-/Cl- antiporter by cAMP increases the pHi and switches off the protein kinase C-mediated regulation. Cyclic AMP 42-46 glucose-6-phosphate isomerase Homo sapiens 61-64 2848021-1 1988 Two mechanisms are involved in the regulation of the intracellular pH (pHi) of aortic smooth muscle cells: the Na+/H+ antiporter and a Na+-independent HCO3-/Cl- antiporter. Bicarbonates 151-155 glucose-6-phosphate isomerase Homo sapiens 71-74 2849306-5 1988 In the absence of CO2-HCO3-, mesangial cells that are acid loaded by an NH+4 prepulse exhibit a spontaneous intracellular pH (pHi) recovery that is blocked either by ethylisopropylamiloride (EIPA) or removal of external Na+. ethylisopropylamiloride 166-189 glucose-6-phosphate isomerase Homo sapiens 122-124 3202154-3 1988 We used the pH-sensitive dye 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) to further characterize the mechanisms of intracellular pH (pHi) regulation in renal mesangial cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 29-72 glucose-6-phosphate isomerase Homo sapiens 12-14 2849306-5 1988 In the absence of CO2-HCO3-, mesangial cells that are acid loaded by an NH+4 prepulse exhibit a spontaneous intracellular pH (pHi) recovery that is blocked either by ethylisopropylamiloride (EIPA) or removal of external Na+. ethylisopropylamiloride 166-189 glucose-6-phosphate isomerase Homo sapiens 126-129 3202154-3 1988 We used the pH-sensitive dye 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) to further characterize the mechanisms of intracellular pH (pHi) regulation in renal mesangial cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 29-72 glucose-6-phosphate isomerase Homo sapiens 137-139 2849306-5 1988 In the absence of CO2-HCO3-, mesangial cells that are acid loaded by an NH+4 prepulse exhibit a spontaneous intracellular pH (pHi) recovery that is blocked either by ethylisopropylamiloride (EIPA) or removal of external Na+. ethylisopropylamiloride 191-195 glucose-6-phosphate isomerase Homo sapiens 122-124 3202154-3 1988 We used the pH-sensitive dye 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) to further characterize the mechanisms of intracellular pH (pHi) regulation in renal mesangial cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 74-79 glucose-6-phosphate isomerase Homo sapiens 12-14 3202154-3 1988 We used the pH-sensitive dye 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) to further characterize the mechanisms of intracellular pH (pHi) regulation in renal mesangial cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 74-79 glucose-6-phosphate isomerase Homo sapiens 137-139 2849306-5 1988 In the absence of CO2-HCO3-, mesangial cells that are acid loaded by an NH+4 prepulse exhibit a spontaneous intracellular pH (pHi) recovery that is blocked either by ethylisopropylamiloride (EIPA) or removal of external Na+. ethylisopropylamiloride 191-195 glucose-6-phosphate isomerase Homo sapiens 126-129 3202154-3 1988 We used the pH-sensitive dye 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) to further characterize the mechanisms of intracellular pH (pHi) regulation in renal mesangial cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 74-79 glucose-6-phosphate isomerase Homo sapiens 141-144 2849306-7 1988 When the cells are switched from a N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solution to one containing CO2-HCO3-, there is an abrupt acidification due to CO2 entry, which is followed by a spontaneous recovery of pHi to a steady-state value higher than that prevailing in HEPES. HEPES 35-86 glucose-6-phosphate isomerase Homo sapiens 240-243 3202154-8 1988 In this study, we examined the ionic dependencies of pHi-regulatory mechanisms in the presence of CO2-HCO3-. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 98-101 glucose-6-phosphate isomerase Homo sapiens 53-56 3202154-8 1988 In this study, we examined the ionic dependencies of pHi-regulatory mechanisms in the presence of CO2-HCO3-. Bicarbonates 102-106 glucose-6-phosphate isomerase Homo sapiens 53-56 2849306-7 1988 When the cells are switched from a N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solution to one containing CO2-HCO3-, there is an abrupt acidification due to CO2 entry, which is followed by a spontaneous recovery of pHi to a steady-state value higher than that prevailing in HEPES. HEPES 88-93 glucose-6-phosphate isomerase Homo sapiens 240-243 2849306-7 1988 When the cells are switched from a N-2-hydroxyethylpiperazine-N"-2-ethanesulfonic acid (HEPES)-buffered solution to one containing CO2-HCO3-, there is an abrupt acidification due to CO2 entry, which is followed by a spontaneous recovery of pHi to a steady-state value higher than that prevailing in HEPES. Bicarbonates 135-139 glucose-6-phosphate isomerase Homo sapiens 240-243 2849306-17 1988 This mechanism also accounts for the increase in steady-state pHi on addition of CO2-HCO3-. Bicarbonates 85-89 glucose-6-phosphate isomerase Homo sapiens 62-65 3191738-3 1988 The stomach wall pH was calculated from the PCO2 in gastric juice and arterial bicarbonate concentration using the Henderson-Hasselbalch equation. Bicarbonates 79-90 glucose-6-phosphate isomerase Homo sapiens 17-19 3265891-5 1988 The measured values of the pHi recovery rates was 0.06 +/- 0.01 delta pHi/min (n = 5) in 3 mM HEPES and was 0.18 +/- 0.06 delta pHi/min (n = 13) in 30 mM HEPES, pHo 7.35. HEPES 94-99 glucose-6-phosphate isomerase Homo sapiens 27-30 3265891-5 1988 The measured values of the pHi recovery rates was 0.06 +/- 0.01 delta pHi/min (n = 5) in 3 mM HEPES and was 0.18 +/- 0.06 delta pHi/min (n = 13) in 30 mM HEPES, pHo 7.35. HEPES 154-159 glucose-6-phosphate isomerase Homo sapiens 27-30 3265891-6 1988 The Na+-H+ exchange inhibitor amiloride (2 mM) slightly reduced pHi recovery rate. Amiloride 30-39 glucose-6-phosphate isomerase Homo sapiens 64-67 3219729-0 1988 Brain pH in migraine: an in vivo phosphorus-31 magnetic resonance spectroscopy study. Phosphorus 33-43 glucose-6-phosphate isomerase Homo sapiens 6-8 3219729-1 1988 The intracellular pH (pHi) of cerebral cortex was measured in migraine patients by use of in vivo phosphorus-31 NMR spectroscopy. Phosphorus 98-108 glucose-6-phosphate isomerase Homo sapiens 18-20 3219729-1 1988 The intracellular pH (pHi) of cerebral cortex was measured in migraine patients by use of in vivo phosphorus-31 NMR spectroscopy. Phosphorus 98-108 glucose-6-phosphate isomerase Homo sapiens 22-25 2850931-3 1988 The 1,4-dihydropyridine VSCC antagonist (-) 202-791 (0.1-1.0 microM) inhibited the GPI twitch height, reduced contractions to exogenous ACh, but failed to affect ACh release. 1,4-dihydropyridine 4-23 glucose-6-phosphate isomerase Homo sapiens 83-86 3265144-4 1988 We found that due to the asymmetric permeability properties of apical and basolateral cell membranes to HCO3- and NH+4, the direction of the variations in pHi was dependent on the side of addition of the acid or alkali load. Bicarbonates 104-108 glucose-6-phosphate isomerase Homo sapiens 155-158 3265144-6 1988 The addition of 15 mmol/liter NH4Cl to control Ringer on the apical side caused an immediate intracellular alkalinization that lasted up to 30 min; subsequent removal of NH4Cl resulted in a reversible fall in pHi, whereas basolateral addition of NH4Cl produced a prolonged intracellular acidosis. Ammonium Chloride 30-35 glucose-6-phosphate isomerase Homo sapiens 209-212 3265144-6 1988 The addition of 15 mmol/liter NH4Cl to control Ringer on the apical side caused an immediate intracellular alkalinization that lasted up to 30 min; subsequent removal of NH4Cl resulted in a reversible fall in pHi, whereas basolateral addition of NH4Cl produced a prolonged intracellular acidosis. Ammonium Chloride 170-175 glucose-6-phosphate isomerase Homo sapiens 209-212 3265144-6 1988 The addition of 15 mmol/liter NH4Cl to control Ringer on the apical side caused an immediate intracellular alkalinization that lasted up to 30 min; subsequent removal of NH4Cl resulted in a reversible fall in pHi, whereas basolateral addition of NH4Cl produced a prolonged intracellular acidosis. Ammonium Chloride 170-175 glucose-6-phosphate isomerase Homo sapiens 209-212 3076850-2 1988 It is related to several other peptides including PHI (peptide with N-terminal histidine and C-terminal isoleucine amide), secretin, glucagon, and has some sequences similar to those of growth hormone releasing hormone (Fig. Histidine 79-88 glucose-6-phosphate isomerase Homo sapiens 50-53 3171590-0 1988 Intracellular pH, lactate, and energy metabolism in neonatal brain during partial ischemia measured in vivo by 31P and 1H nuclear magnetic resonance spectroscopy. ET bromodomain inhibitor 111-114 glucose-6-phosphate isomerase Homo sapiens 14-16 2847550-5 1988 This response of pHi is inhibited or abolished when the apical side is treated with 10(-3) M 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 93-141 glucose-6-phosphate isomerase Homo sapiens 17-20 2847550-5 1988 This response of pHi is inhibited or abolished when the apical side is treated with 10(-3) M 4,4"-diisothiocyanostilbene-2,2"-disulfonic acid (DIDS). 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 143-147 glucose-6-phosphate isomerase Homo sapiens 17-20 2847550-10 1988 The inhibitory effect of apically applied DIDS suggests that the voltage dependent changes in pHi are related to movement of HCO3 (or OH) ions across the apical cell membrane. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 42-46 glucose-6-phosphate isomerase Homo sapiens 94-97 3169488-6 1988 Removal of Na+ from and addition of amiloride to the serosal perfusate during exposure to serosal pH 6.0 induced further acidification of pHi, suggesting that in this acidotic situation (with very low ambient HCO3- concentration) a Na+/H+ exchanger does contribute to the maintenance of steady-state pHi. Amiloride 36-45 glucose-6-phosphate isomerase Homo sapiens 98-100 3169488-6 1988 Removal of Na+ from and addition of amiloride to the serosal perfusate during exposure to serosal pH 6.0 induced further acidification of pHi, suggesting that in this acidotic situation (with very low ambient HCO3- concentration) a Na+/H+ exchanger does contribute to the maintenance of steady-state pHi. Amiloride 36-45 glucose-6-phosphate isomerase Homo sapiens 138-141 3169488-6 1988 Removal of Na+ from and addition of amiloride to the serosal perfusate during exposure to serosal pH 6.0 induced further acidification of pHi, suggesting that in this acidotic situation (with very low ambient HCO3- concentration) a Na+/H+ exchanger does contribute to the maintenance of steady-state pHi. Amiloride 36-45 glucose-6-phosphate isomerase Homo sapiens 300-303 3169488-6 1988 Removal of Na+ from and addition of amiloride to the serosal perfusate during exposure to serosal pH 6.0 induced further acidification of pHi, suggesting that in this acidotic situation (with very low ambient HCO3- concentration) a Na+/H+ exchanger does contribute to the maintenance of steady-state pHi. Bicarbonates 209-213 glucose-6-phosphate isomerase Homo sapiens 98-100 3169488-7 1988 Increased PCO2 (10% vol/vol in the gas) in a slightly acidic milieu (mimicking mucosal ischemia) likewise acidified pHi to 6.73 +/- 0.05. pco2 10-14 glucose-6-phosphate isomerase Homo sapiens 116-119 3221384-5 1988 Prepulsing with 20 mM NH4 in the presence of CO2/HCO3 typically reduced pHi to only about neutral, whereas 50 mM reduced pHi to 6.7-6.8. Ammonia 22-25 glucose-6-phosphate isomerase Homo sapiens 72-75 3221384-5 1988 Prepulsing with 20 mM NH4 in the presence of CO2/HCO3 typically reduced pHi to only about neutral, whereas 50 mM reduced pHi to 6.7-6.8. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 45-48 glucose-6-phosphate isomerase Homo sapiens 72-75 3221384-5 1988 Prepulsing with 20 mM NH4 in the presence of CO2/HCO3 typically reduced pHi to only about neutral, whereas 50 mM reduced pHi to 6.7-6.8. Bicarbonates 49-53 glucose-6-phosphate isomerase Homo sapiens 72-75 3171590-0 1988 Intracellular pH, lactate, and energy metabolism in neonatal brain during partial ischemia measured in vivo by 31P and 1H nuclear magnetic resonance spectroscopy. Hydrogen 119-121 glucose-6-phosphate isomerase Homo sapiens 14-16 3221384-6 1988 In the nominal absence of CO2/HCO3, 20 mM NH4 reduced pHi to about 6.7 from which recovery took place at about 58% of the rate seen in different cells in the presence of CO2/HCO3. Ammonia 42-45 glucose-6-phosphate isomerase Homo sapiens 54-57 3221384-7 1988 In the presence of CO2/HCO3, cells prepulsed with 50 mM NH4 had fully recovered to an average pHi of 7.22 +/- 0.04 about 90 min after removal of NH4. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 19-22 glucose-6-phosphate isomerase Homo sapiens 94-97 3221384-7 1988 In the presence of CO2/HCO3, cells prepulsed with 50 mM NH4 had fully recovered to an average pHi of 7.22 +/- 0.04 about 90 min after removal of NH4. Bicarbonates 23-27 glucose-6-phosphate isomerase Homo sapiens 94-97 3221384-7 1988 In the presence of CO2/HCO3, cells prepulsed with 50 mM NH4 had fully recovered to an average pHi of 7.22 +/- 0.04 about 90 min after removal of NH4. Ammonia 56-59 glucose-6-phosphate isomerase Homo sapiens 94-97 3221384-8 1988 However, 90 min after removal of 20 mM NH4 in the absence of CO2/HCO3, average pHi was significantly less (7.05 +/- 0.03). Ammonia 39-42 glucose-6-phosphate isomerase Homo sapiens 79-82 3221384-11 1988 The data provide significant support for an important role of HCO3 in the control of pHi in fast-twitch muscle. Bicarbonates 62-66 glucose-6-phosphate isomerase Homo sapiens 85-88 3171590-4 1988 In four piglets, whose arterial blood glucose rose above control, brain lactate exceeded 20 mumol g-1 with corresponding decreases in pHi of greater than 0.7 units compared to control levels. Lactic Acid 72-79 glucose-6-phosphate isomerase Homo sapiens 134-137 3165863-1 1988 Monocytic differentiation of U937 cells induced by retinoic acid is accompanied by a 0.2-pH-unit cell alkalinisation. Tretinoin 51-64 glucose-6-phosphate isomerase Homo sapiens 89-91 2844320-6 1988 Addition of Ni2+ also suppressed stimulus-induced pHi increase. Nickel(2+) 12-16 glucose-6-phosphate isomerase Homo sapiens 50-53 2844320-7 1988 A treatment of platelets by Ca-ionophore A23187 caused a rise of pHi without its initial decrease; in medium without Ca2+ the changes of pHi were inhibited. Calcimycin 41-47 glucose-6-phosphate isomerase Homo sapiens 65-68 2844320-7 1988 A treatment of platelets by Ca-ionophore A23187 caused a rise of pHi without its initial decrease; in medium without Ca2+ the changes of pHi were inhibited. Calcimycin 41-47 glucose-6-phosphate isomerase Homo sapiens 137-140 3175363-6 1988 This quantity exhibited a three fold change between pHi 5.5 and 8.5, with extracellular chloride concentration [Cl-]e fixed at 140 mM. Chlorides 88-96 glucose-6-phosphate isomerase Homo sapiens 52-55 3165863-2 1988 The effect of retinoic acid on intracellular pH (pHi) develops slowly and it precedes the differentiation of the cells by 24 h. Heterogeneity in cellular pHi values was assessed using flow cytometry. Tretinoin 14-27 glucose-6-phosphate isomerase Homo sapiens 45-47 3165863-2 1988 The effect of retinoic acid on intracellular pH (pHi) develops slowly and it precedes the differentiation of the cells by 24 h. Heterogeneity in cellular pHi values was assessed using flow cytometry. Tretinoin 14-27 glucose-6-phosphate isomerase Homo sapiens 49-52 3165863-2 1988 The effect of retinoic acid on intracellular pH (pHi) develops slowly and it precedes the differentiation of the cells by 24 h. Heterogeneity in cellular pHi values was assessed using flow cytometry. Tretinoin 14-27 glucose-6-phosphate isomerase Homo sapiens 154-157 2842350-1 1988 We examined the role of superoxide in the increase in intracellular pH (pHi) of human histiocytic leukemia U937 cells treated with 4 beta-phorbol-12,13-didecanoate (4 beta-PDD) or serum. Superoxides 24-34 glucose-6-phosphate isomerase Homo sapiens 72-75 3403517-0 1988 Role of chloride/bicarbonate antiport in the control of cytosolic pH. Chlorides 8-16 glucose-6-phosphate isomerase Homo sapiens 66-68 3403517-0 1988 Role of chloride/bicarbonate antiport in the control of cytosolic pH. Bicarbonates 17-28 glucose-6-phosphate isomerase Homo sapiens 66-68 3403517-3 1988 Transport of HCO3- was estimated from its effect on intracellular pH (pHi) measured with the fluorescent probe 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein. Bicarbonates 13-17 glucose-6-phosphate isomerase Homo sapiens 66-68 3403517-3 1988 Transport of HCO3- was estimated from its effect on intracellular pH (pHi) measured with the fluorescent probe 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein. Bicarbonates 13-17 glucose-6-phosphate isomerase Homo sapiens 70-73 3403517-3 1988 Transport of HCO3- was estimated from its effect on intracellular pH (pHi) measured with the fluorescent probe 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 111-157 glucose-6-phosphate isomerase Homo sapiens 66-68 3403517-3 1988 Transport of HCO3- was estimated from its effect on intracellular pH (pHi) measured with the fluorescent probe 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 111-157 glucose-6-phosphate isomerase Homo sapiens 70-73 3403517-6 1988 The Na+-independent HCO3-/Cl- antiport was found to be highly pHi-dependent in a number of cell lines, whereas in others this was not the case. Bicarbonates 20-24 glucose-6-phosphate isomerase Homo sapiens 62-65 2842350-1 1988 We examined the role of superoxide in the increase in intracellular pH (pHi) of human histiocytic leukemia U937 cells treated with 4 beta-phorbol-12,13-didecanoate (4 beta-PDD) or serum. 4 beta-phorbol-12,13-didecanoate 131-163 glucose-6-phosphate isomerase Homo sapiens 72-75 2842350-2 1988 4 beta-PDD or serum induced a rapid increase in pHi, and antioxidants such as superoxide dismutase (SOD), vitamin E, and butylated hydroxyanisole (BHA) were found to inhibit the amiloride-sensitive increase in pHi induced by 4 beta-PDD. Butylated Hydroxyanisole 121-145 glucose-6-phosphate isomerase Homo sapiens 210-213 2842350-2 1988 4 beta-PDD or serum induced a rapid increase in pHi, and antioxidants such as superoxide dismutase (SOD), vitamin E, and butylated hydroxyanisole (BHA) were found to inhibit the amiloride-sensitive increase in pHi induced by 4 beta-PDD. Butylated Hydroxyanisole 147-150 glucose-6-phosphate isomerase Homo sapiens 210-213 2842350-2 1988 4 beta-PDD or serum induced a rapid increase in pHi, and antioxidants such as superoxide dismutase (SOD), vitamin E, and butylated hydroxyanisole (BHA) were found to inhibit the amiloride-sensitive increase in pHi induced by 4 beta-PDD. Amiloride 178-187 glucose-6-phosphate isomerase Homo sapiens 48-51 2842350-2 1988 4 beta-PDD or serum induced a rapid increase in pHi, and antioxidants such as superoxide dismutase (SOD), vitamin E, and butylated hydroxyanisole (BHA) were found to inhibit the amiloride-sensitive increase in pHi induced by 4 beta-PDD. Amiloride 178-187 glucose-6-phosphate isomerase Homo sapiens 210-213 2842350-4 1988 Also, a superoxide-generating system, xanthine-xanthine oxidase (X-XOD), increased pHi of U937 cells as much as 4 beta-PDD or serum. Superoxides 8-18 glucose-6-phosphate isomerase Homo sapiens 83-86 2842350-5 1988 From these findings, it appears that superoxide is the basis for the modulation of pHi. Superoxides 37-47 glucose-6-phosphate isomerase Homo sapiens 83-86 3174385-3 1988 (3) pHi-Recovery after an acute intracellular acid load (by means of NH4Cl-prepulse) was reversibly blocked by 1 mM amiloride and was dependent on the presence of sodium. Ammonium Chloride 69-74 glucose-6-phosphate isomerase Homo sapiens 4-7 2455658-2 1988 12-O-Tetradecanoyl phorbol-13-acetate (PMA), butyrate, interferon, retinoic acid and 1,25-dihydroxyvitamin D3 all increased pHi. Tetradecanoylphorbol Acetate 0-37 glucose-6-phosphate isomerase Homo sapiens 124-127 2455658-2 1988 12-O-Tetradecanoyl phorbol-13-acetate (PMA), butyrate, interferon, retinoic acid and 1,25-dihydroxyvitamin D3 all increased pHi. Tetradecanoylphorbol Acetate 39-42 glucose-6-phosphate isomerase Homo sapiens 124-127 2455658-2 1988 12-O-Tetradecanoyl phorbol-13-acetate (PMA), butyrate, interferon, retinoic acid and 1,25-dihydroxyvitamin D3 all increased pHi. Butyrates 45-53 glucose-6-phosphate isomerase Homo sapiens 124-127 2455658-2 1988 12-O-Tetradecanoyl phorbol-13-acetate (PMA), butyrate, interferon, retinoic acid and 1,25-dihydroxyvitamin D3 all increased pHi. Tretinoin 67-80 glucose-6-phosphate isomerase Homo sapiens 124-127 2455658-2 1988 12-O-Tetradecanoyl phorbol-13-acetate (PMA), butyrate, interferon, retinoic acid and 1,25-dihydroxyvitamin D3 all increased pHi. Calcitriol 85-109 glucose-6-phosphate isomerase Homo sapiens 124-127 3174385-3 1988 (3) pHi-Recovery after an acute intracellular acid load (by means of NH4Cl-prepulse) was reversibly blocked by 1 mM amiloride and was dependent on the presence of sodium. Amiloride 116-125 glucose-6-phosphate isomerase Homo sapiens 4-7 3174385-3 1988 (3) pHi-Recovery after an acute intracellular acid load (by means of NH4Cl-prepulse) was reversibly blocked by 1 mM amiloride and was dependent on the presence of sodium. Sodium 163-169 glucose-6-phosphate isomerase Homo sapiens 4-7 3174385-4 1988 The velocity of pHi recovery increased with increasing sodium concentrations with an apparent Km for external sodium of about 30 mM and a Vmax of about 0.35 pH units/min. Sodium 55-61 glucose-6-phosphate isomerase Homo sapiens 16-19 3174385-4 1988 The velocity of pHi recovery increased with increasing sodium concentrations with an apparent Km for external sodium of about 30 mM and a Vmax of about 0.35 pH units/min. Sodium 110-116 glucose-6-phosphate isomerase Homo sapiens 16-19 3174385-8 1988 (5) Removal of chloride during pHi recovery from an alkaline load (imposed by acetate prepulse) stopped and reversed pHi backregulation. Chlorides 15-23 glucose-6-phosphate isomerase Homo sapiens 31-34 3174385-8 1988 (5) Removal of chloride during pHi recovery from an alkaline load (imposed by acetate prepulse) stopped and reversed pHi backregulation. Chlorides 15-23 glucose-6-phosphate isomerase Homo sapiens 117-120 3174385-8 1988 (5) Removal of chloride during pHi recovery from an alkaline load (imposed by acetate prepulse) stopped and reversed pHi backregulation. Acetates 78-85 glucose-6-phosphate isomerase Homo sapiens 31-34 3174385-8 1988 (5) Removal of chloride during pHi recovery from an alkaline load (imposed by acetate prepulse) stopped and reversed pHi backregulation. Acetates 78-85 glucose-6-phosphate isomerase Homo sapiens 117-120 3402185-6 1988 Urinary dinor-metabolites of TxB2 and PGI2 (Tx-M and PGI-M, respectively) were estimated by gas chromatography/negative ion-chemical ionization mass spectrometry in samples collected prior to, during and immediately after 20 min of severe treadmill exertion. S-(2-(N,N-diisopropylamino)ethyl)isothiourea 8-13 glucose-6-phosphate isomerase Homo sapiens 38-41 3382644-2 1988 Lowering pHi by 1 unit or less resulted in a 20-fold stimulation of 36Cl efflux which occurred relatively rapidly and which could be inhibited by 90-95% by 4-acetamido-4"-isothio-cyanostilbene-2,2"-disulfonic acid (SITS). 4-acetamido-4"-isothio-cyanostilbene-2,2"-disulfonic acid 156-213 glucose-6-phosphate isomerase Homo sapiens 9-12 3262155-4 1988 Resting intracellular pH (pHi) in 20 mmol l-1 HEPES buffer was 7.18 +/- 0.015 (S.E. HEPES 46-51 glucose-6-phosphate isomerase Homo sapiens 26-29 3262155-8 1988 A reversible decrease in pHi and an increase in intracellular anion levels was observed when L-lactate replaced chloride in equimolar amounts. L-lactate 93-102 glucose-6-phosphate isomerase Homo sapiens 25-28 3262155-8 1988 A reversible decrease in pHi and an increase in intracellular anion levels was observed when L-lactate replaced chloride in equimolar amounts. Chlorides 112-120 glucose-6-phosphate isomerase Homo sapiens 25-28 3262155-11 1988 The rate and steady-state change in pHi and anion level was a function of both extracellular pH and L-lactate concentration, providing evidence for the coupled movement of lactate and proton equivalents. L-lactate 100-109 glucose-6-phosphate isomerase Homo sapiens 36-39 3262155-11 1988 The rate and steady-state change in pHi and anion level was a function of both extracellular pH and L-lactate concentration, providing evidence for the coupled movement of lactate and proton equivalents. Lactic Acid 102-109 glucose-6-phosphate isomerase Homo sapiens 36-39 3262155-13 1988 The initial rate of uptake of L-lactate, as measured by the change of pHi, was a non-linear function of the extracellular L-lactate concentration at extracellular pH 6.8 and 7.35. L-lactate 30-39 glucose-6-phosphate isomerase Homo sapiens 70-73 3262155-13 1988 The initial rate of uptake of L-lactate, as measured by the change of pHi, was a non-linear function of the extracellular L-lactate concentration at extracellular pH 6.8 and 7.35. L-lactate 122-131 glucose-6-phosphate isomerase Homo sapiens 70-73 3262155-17 1988 The non-linear relationship between the initial rate of change in pHi and extracellular L-lactate was well fitted by a curve defining uptake as the sum of a carrier process displaying Michaelis-Menten kinetics and a passive diffusion component. L-lactate 88-97 glucose-6-phosphate isomerase Homo sapiens 66-69 3262155-20 1988 The initial rate of change of pHi in the presence of L-lactate was significantly inhibited 39.1 +/- 6.2% by 2-5 mmol l-1 alpha-cyano-4-hydroxycinnamate (n = 9; P less than 0.05, paired t test). L-lactate 53-62 glucose-6-phosphate isomerase Homo sapiens 30-33 3262155-20 1988 The initial rate of change of pHi in the presence of L-lactate was significantly inhibited 39.1 +/- 6.2% by 2-5 mmol l-1 alpha-cyano-4-hydroxycinnamate (n = 9; P less than 0.05, paired t test). l-1 alpha-cyano-4-hydroxycinnamate 117-151 glucose-6-phosphate isomerase Homo sapiens 30-33 3262155-24 1988 The initial rate of change of pHi induced by L-lactate was not affected by 20-100 mumol l-1 4-acetamido-4"-isothiocyanostilbene-2,2"-disulphonic acid (SITS). L-lactate 45-54 glucose-6-phosphate isomerase Homo sapiens 30-33 3402185-9 1988 The basal excretion of PGI-M was 142 +/- 25 pg/mg creatinine. Creatinine 50-60 glucose-6-phosphate isomerase Homo sapiens 23-26 3402185-11 1988 During the recovery period the outflow of PGI-M was significantly higher than at rest (482 +/- 145 pg/mg creatinine; P less than 0.01). Creatinine 105-115 glucose-6-phosphate isomerase Homo sapiens 42-45 3354650-2 1988 After exposure of cells to the tripeptide N-formyl-methionyl-leucyl-phenylalanine (FMLP), the influx of Li+ was measured by flame photometry and correlated with changes in intracellular pH (pHi) derived from the equilibrium distribution of 5,5-dimethyloxazolidine-2,4-dione. Dimethadione 240-273 glucose-6-phosphate isomerase Homo sapiens 190-193 2836231-3 1988 When extracellular Na+ was substituted by equimolar choline+, pHi decreased by about 0.2 units. Choline 52-60 glucose-6-phosphate isomerase Homo sapiens 62-65 2456757-2 1988 The internal pH (pHi) of cytoplasts, derived from human neutrophils, falls 0.05 pH units upon activation of the superoxide-generating NADPH oxidase. Superoxides 112-122 glucose-6-phosphate isomerase Homo sapiens 17-20 2456757-3 1988 The decrease in pHi is absent in diphenyleneiodonium-treated cytoplasts and therefore it is likely to arise directly from the activity of the oxidase. diphenyleneiodonium 33-52 glucose-6-phosphate isomerase Homo sapiens 16-19 3354650-7 1988 The influx of Li+, as well as the increase in pHi in 140 mM Li+ medium, was competitively inhibited by amiloride (Ki approximately equal to 9 microM). li+ medium 60-70 glucose-6-phosphate isomerase Homo sapiens 46-49 3354650-7 1988 The influx of Li+, as well as the increase in pHi in 140 mM Li+ medium, was competitively inhibited by amiloride (Ki approximately equal to 9 microM). Amiloride 103-112 glucose-6-phosphate isomerase Homo sapiens 46-49 3352744-6 1988 We have cloned the gene for pig muscle phosphohexose isomerase (PHI) (EC 5.3.1.9) which catalyses the conversion of glucose-6-phosphate to fructose-6-phosphate, an obligatory step in glycolysis, and determined its amino-acid sequence. Glucose-6-Phosphate 116-135 glucose-6-phosphate isomerase Homo sapiens 39-62 2831223-3 1988 Several observations indicate that the exchanger is regulated by protein kinase C. (i) Inhibitors of protein kinase C (trifluoperazine, sphingosine) inhibit the increase in pHi seen during thrombin stimulation as well as Ca2+ mobilization; artificially increasing pHi by monensin or NH4Cl then restores Ca2+ mobilization. Monensin 271-279 glucose-6-phosphate isomerase Homo sapiens 173-176 2831223-3 1988 Several observations indicate that the exchanger is regulated by protein kinase C. (i) Inhibitors of protein kinase C (trifluoperazine, sphingosine) inhibit the increase in pHi seen during thrombin stimulation as well as Ca2+ mobilization; artificially increasing pHi by monensin or NH4Cl then restores Ca2+ mobilization. Ammonium Chloride 283-288 glucose-6-phosphate isomerase Homo sapiens 173-176 2831223-4 1988 (ii) Direct activation of protein kinase C by 1-oleoyl-2-acetylglycerol initiates an increase in pHi that depends on the presence of extracellular Na+ and is sensitive to inhibition by ethylisopropylamiloride. 1-oleoyl-2-acetylglycerol 46-71 glucose-6-phosphate isomerase Homo sapiens 97-100 2831223-4 1988 (ii) Direct activation of protein kinase C by 1-oleoyl-2-acetylglycerol initiates an increase in pHi that depends on the presence of extracellular Na+ and is sensitive to inhibition by ethylisopropylamiloride. ethylisopropylamiloride 185-208 glucose-6-phosphate isomerase Homo sapiens 97-100 2831223-6 1988 In the absence of thrombin an increase in pHi, either induced artificially (by addition of the ionophores nigericin or monensin) or via activation of protein kinase C (by addition of 1-oleoyl-2-acetylglycerol), does not induce Ca2+ mobilization. Nigericin 106-115 glucose-6-phosphate isomerase Homo sapiens 42-45 2831223-6 1988 In the absence of thrombin an increase in pHi, either induced artificially (by addition of the ionophores nigericin or monensin) or via activation of protein kinase C (by addition of 1-oleoyl-2-acetylglycerol), does not induce Ca2+ mobilization. Monensin 119-127 glucose-6-phosphate isomerase Homo sapiens 42-45 2831223-6 1988 In the absence of thrombin an increase in pHi, either induced artificially (by addition of the ionophores nigericin or monensin) or via activation of protein kinase C (by addition of 1-oleoyl-2-acetylglycerol), does not induce Ca2+ mobilization. 1-oleoyl-2-acetylglycerol 183-208 glucose-6-phosphate isomerase Homo sapiens 42-45 2831223-3 1988 Several observations indicate that the exchanger is regulated by protein kinase C. (i) Inhibitors of protein kinase C (trifluoperazine, sphingosine) inhibit the increase in pHi seen during thrombin stimulation as well as Ca2+ mobilization; artificially increasing pHi by monensin or NH4Cl then restores Ca2+ mobilization. Trifluoperazine 119-134 glucose-6-phosphate isomerase Homo sapiens 173-176 2831223-3 1988 Several observations indicate that the exchanger is regulated by protein kinase C. (i) Inhibitors of protein kinase C (trifluoperazine, sphingosine) inhibit the increase in pHi seen during thrombin stimulation as well as Ca2+ mobilization; artificially increasing pHi by monensin or NH4Cl then restores Ca2+ mobilization. Trifluoperazine 119-134 glucose-6-phosphate isomerase Homo sapiens 264-267 2831223-3 1988 Several observations indicate that the exchanger is regulated by protein kinase C. (i) Inhibitors of protein kinase C (trifluoperazine, sphingosine) inhibit the increase in pHi seen during thrombin stimulation as well as Ca2+ mobilization; artificially increasing pHi by monensin or NH4Cl then restores Ca2+ mobilization. Sphingosine 136-147 glucose-6-phosphate isomerase Homo sapiens 173-176 2831223-3 1988 Several observations indicate that the exchanger is regulated by protein kinase C. (i) Inhibitors of protein kinase C (trifluoperazine, sphingosine) inhibit the increase in pHi seen during thrombin stimulation as well as Ca2+ mobilization; artificially increasing pHi by monensin or NH4Cl then restores Ca2+ mobilization. Sphingosine 136-147 glucose-6-phosphate isomerase Homo sapiens 264-267 3352744-6 1988 We have cloned the gene for pig muscle phosphohexose isomerase (PHI) (EC 5.3.1.9) which catalyses the conversion of glucose-6-phosphate to fructose-6-phosphate, an obligatory step in glycolysis, and determined its amino-acid sequence. fructose-6-phosphate 139-159 glucose-6-phosphate isomerase Homo sapiens 39-62 2462478-5 1988 In high NaCl-containing mucosal solutions or in short-circuit conditions, the J(net)Na becomes uncoupled from J(net)H and proceeds mainly via the principal cells in the epithelium, in which pHi is regulated by basolateral Na+/H+ and Cl-/HCO3- exchangers. Sodium Chloride 8-12 glucose-6-phosphate isomerase Homo sapiens 190-193 3347270-12 1988 We have now explored the role of ATP in pHi regulation by dialysing axons with the ATP analogue ATP-gamma-S. adenosine 5'-O-(3-thiotriphosphate) 96-107 glucose-6-phosphate isomerase Homo sapiens 40-43 2828086-2 1988 Here we report that pHi, measured by the digitonin null-point method, is constant during G1-phase and the G1/S-phase transition and decreases in early S-phase. Digitonin 41-50 glucose-6-phosphate isomerase Homo sapiens 20-23 2828086-3 1988 In addition pHi is shown to be most sensitive to the diuretic amiloride in the G1/S-phase transition, in agreement with the ion influx data. Amiloride 62-71 glucose-6-phosphate isomerase Homo sapiens 12-15 2849528-1 1988 Intracellular pH (pHi) in sheep cardiac Purkinje fibres is controlled by sarcolemmal Na+/H+ and Cl-/HCO3- exchange. Bicarbonates 100-104 glucose-6-phosphate isomerase Homo sapiens 18-21 2849528-5 1988 Rate-dependent pHi changes are inhibited following inhibition of glycolysis, indicating that they are caused by accumulation of lactic acid. Lactic Acid 128-139 glucose-6-phosphate isomerase Homo sapiens 15-18 2849528-6 1988 In some cases, the efflux of lactic acid may provide a faster method for recovery of pHi from a metabolic acidosis than that provided by Na+/H+ exchange. Lactic Acid 29-40 glucose-6-phosphate isomerase Homo sapiens 85-88 3347270-2 1988 Sodium-dependent Cl-HCO3 exchange is the dominant mechanism of pHi regulation in the invertebrate cells examined, and also occurs in mammalian cells. Sodium 0-6 glucose-6-phosphate isomerase Homo sapiens 63-66 3347270-2 1988 Sodium-dependent Cl-HCO3 exchange is the dominant mechanism of pHi regulation in the invertebrate cells examined, and also occurs in mammalian cells. Bicarbonates 20-24 glucose-6-phosphate isomerase Homo sapiens 63-66 3347270-12 1988 We have now explored the role of ATP in pHi regulation by dialysing axons with the ATP analogue ATP-gamma-S. Adenosine Triphosphate 33-36 glucose-6-phosphate isomerase Homo sapiens 40-43 3123519-2 1988 For the same changes in external pH, changes in [HCO3] and PCO2 affected cell pH similarly ([HCO3]: pHi/pHe = 0.67, PCO2: pHi/pHe = 0.64, NS). Bicarbonates 49-53 glucose-6-phosphate isomerase Homo sapiens 100-103 3123519-2 1988 For the same changes in external pH, changes in [HCO3] and PCO2 affected cell pH similarly ([HCO3]: pHi/pHe = 0.67, PCO2: pHi/pHe = 0.64, NS). Bicarbonates 93-97 glucose-6-phosphate isomerase Homo sapiens 100-103 3123519-2 1988 For the same changes in external pH, changes in [HCO3] and PCO2 affected cell pH similarly ([HCO3]: pHi/pHe = 0.67, PCO2: pHi/pHe = 0.64, NS). pco2 116-120 glucose-6-phosphate isomerase Homo sapiens 122-125 3334878-2 1988 Since it can consume mannose and glucose equivalently in the hereditary deficiencies of hexokinase and phosphoglucose isomerase and since erythrocyte defense against oxidants is impaired in a variety of hereditary hemolytic anemias, we tested the hypothesis that mannose may be a significant alternative to glucose as a fuel for this defense system. Glucose 33-40 glucose-6-phosphate isomerase Homo sapiens 103-127 3261495-2 1988 The pHi was changed in a controlled way, independent of other metabolite levels, by changing the extracellular concentration of the permeant acid carbon dioxide. Carbon Dioxide 146-160 glucose-6-phosphate isomerase Homo sapiens 4-7 2849531-4 1988 Lymphocytes also possess a cation-independent anion (Cl-/HCO3-) exchange system, which, under the appropriate conditions, tends to restore pHi after an alkali load. Bicarbonates 57-61 glucose-6-phosphate isomerase Homo sapiens 139-142 2849531-11 1988 Instead, Cl-/HCO3- exchange may simply stabilize pHi by increasing the dynamic buffering power of the cells. Bicarbonates 13-17 glucose-6-phosphate isomerase Homo sapiens 49-52 2462478-5 1988 In high NaCl-containing mucosal solutions or in short-circuit conditions, the J(net)Na becomes uncoupled from J(net)H and proceeds mainly via the principal cells in the epithelium, in which pHi is regulated by basolateral Na+/H+ and Cl-/HCO3- exchangers. Bicarbonates 237-241 glucose-6-phosphate isomerase Homo sapiens 190-193 2849531-12 1988 Cation-independent Cl-/HCO3- exchange could be involved in pHi regulation only if coupled to a separate mechanism of intracellular Cl- accumulation, such as Na+-K+-2Cl- co-transport or an inward Cl- pump, which have not been detected in lymphoid cells. Bicarbonates 23-27 glucose-6-phosphate isomerase Homo sapiens 59-62 3121373-11 1988 Microinjection of an alkaline Hepes buffer or external application of ammonia, both of which increased pHi, prevented unfertilized oocytes from arresting after formation of the female pronucleus and induced chromosome cycling. alkaline hepes 21-35 glucose-6-phosphate isomerase Homo sapiens 103-106 3121373-11 1988 Microinjection of an alkaline Hepes buffer or external application of ammonia, both of which increased pHi, prevented unfertilized oocytes from arresting after formation of the female pronucleus and induced chromosome cycling. Ammonia 70-77 glucose-6-phosphate isomerase Homo sapiens 103-106 3121373-12 1988 Phosphorylation of S6 was still observed following fertilization or induction of maturation when pHi was decreased by external application of acetate, a treatment which suppressed the emission of polar bodies. Acetates 142-149 glucose-6-phosphate isomerase Homo sapiens 97-100 3257228-0 1988 Calcium-dependent intracellular acidification dominates the pH response to mitogen in human T cells. Calcium 0-7 glucose-6-phosphate isomerase Homo sapiens 60-62 3060326-1 1988 Traditional models of acid-base transport and intracellular pH (pHi) regulation in the renal proximal tubule have been based on the existence of a Na+/H+ exchanger at the luminal membrane and a simple HCO3- conductance at the basolateral membrane. Bicarbonates 201-205 glucose-6-phosphate isomerase Homo sapiens 64-67 3060326-10 1988 Thus, monocarboxylate transport, at least in the nominal absence of HCO3-, can have a major impact on pHi regulation. monocarboxylate 6-21 glucose-6-phosphate isomerase Homo sapiens 102-105 3257228-1 1988 The effects of a phorol ester and a mitogenic lectin on the intracellular pH (pHi) of human T lymphocytes was investigated. phorol ester 17-29 glucose-6-phosphate isomerase Homo sapiens 78-81 3257228-3 1988 This decrease in pHi was magnified in Na+-free medium or in the presence of amiloride analogues, suggesting that activation of Na+/H+ exchange partially counteracts the phytohemagglutinin-induced acidification. Amiloride 76-85 glucose-6-phosphate isomerase Homo sapiens 17-20 3257228-4 1988 The decrease in pHi was dependent on a sustained increase in cytosolic free Ca2+ and could be mimicked by addition of the divalent cation ionophore, ionomycin. Ionomycin 149-158 glucose-6-phosphate isomerase Homo sapiens 16-19 2449521-2 1988 Iontophoretic injection of adenosine 3",5"-cyclic monophosphate (cAMP) into identified neurons elicited a slow transient Na+ current whose amplitude and duration were sensitive to altered intracellular pH (pHi), calmodulin blocking drugs, depolarization, and manipulations of internal and external Ca2+. Cyclic AMP 27-63 glucose-6-phosphate isomerase Homo sapiens 206-209 3691531-5 1987 Bicarbonate induced pHi recovery of the cells after a cellular acidification to pHi = 6.3 provided that Na+ ions were present in the assay medium. Bicarbonates 0-11 glucose-6-phosphate isomerase Homo sapiens 20-23 2449521-2 1988 Iontophoretic injection of adenosine 3",5"-cyclic monophosphate (cAMP) into identified neurons elicited a slow transient Na+ current whose amplitude and duration were sensitive to altered intracellular pH (pHi), calmodulin blocking drugs, depolarization, and manipulations of internal and external Ca2+. Cyclic AMP 65-69 glucose-6-phosphate isomerase Homo sapiens 206-209 2449521-4 1988 Intracellular acidification between resting pHi to several tenths of a pH unit increased the amplitude of the cAMP-stimulated current and prolonged its duration. Cyclic AMP 110-114 glucose-6-phosphate isomerase Homo sapiens 44-47 2449521-9 1988 The immediacy of the increase and the dual acid/basic sensitivity of the response suggest an accommodative process whereby the responsiveness of the cell to cAMP adapts to a maintained pHi. Cyclic AMP 157-161 glucose-6-phosphate isomerase Homo sapiens 185-188 3691531-5 1987 Bicarbonate induced pHi recovery of the cells after a cellular acidification to pHi = 6.3 provided that Na+ ions were present in the assay medium. Bicarbonates 0-11 glucose-6-phosphate isomerase Homo sapiens 80-83 3691531-10 1987 36Cl- efflux experiments showed that the presence of both external Na+ and bicarbonate stimulated the efflux of 36Cl- at a cell pHi of 6.3. Chlorine-36 0-4 glucose-6-phosphate isomerase Homo sapiens 128-131 3691531-10 1987 36Cl- efflux experiments showed that the presence of both external Na+ and bicarbonate stimulated the efflux of 36Cl- at a cell pHi of 6.3. Bicarbonates 75-86 glucose-6-phosphate isomerase Homo sapiens 128-131 3691531-10 1987 36Cl- efflux experiments showed that the presence of both external Na+ and bicarbonate stimulated the efflux of 36Cl- at a cell pHi of 6.3. Chlorine-36 0-5 glucose-6-phosphate isomerase Homo sapiens 128-131 2825425-1 1987 gpI, the predominant varicella-zoster virus (VZV) envelope glycoprotein, was shown to be phosphorylated exclusively on serine and threonine residues, and phosphorylated gpI was detected in isolated virions. Serine 119-125 glucose-6-phosphate isomerase Homo sapiens 0-3 3427448-3 1987 pHi was monitored fluorimetrically with 2",7"-bis-(2-carboxyethyl)-5,6-carboxyfluorescein. 2",7"-bis-(2-carboxyethyl)-5,6-carboxyfluorescein 40-89 glucose-6-phosphate isomerase Homo sapiens 0-3 3427448-4 1987 Upon acidification with sodium propionate, pHi dropped to 6.74 +/- 0.05 (n = 25), and then recovered to levels near the physiological value of 7.23 +/- 0.02 (n = 13). sodium propionate 24-41 glucose-6-phosphate isomerase Homo sapiens 43-46 3427448-6 1987 Both pHi recovery and cell swelling were Na+-dependent, amiloride-sensitive, and inhibited at pHo less than 6.0. Amiloride 56-65 glucose-6-phosphate isomerase Homo sapiens 5-8 2825425-1 1987 gpI, the predominant varicella-zoster virus (VZV) envelope glycoprotein, was shown to be phosphorylated exclusively on serine and threonine residues, and phosphorylated gpI was detected in isolated virions. Threonine 130-139 glucose-6-phosphate isomerase Homo sapiens 0-3 2821820-2 1987 By means of intracellular dialysis, we found that changes of intracellular pH (pHi), but not of extracellular pH, affected ouabain-sensitive Na+ efflux and K+ influx over the pH range of 6.0-8.6. Ouabain 123-130 glucose-6-phosphate isomerase Homo sapiens 75-77 3667622-10 1987 Treatment with Cs+ plus valinomycin (as an alternative method of membrane depolarization) increases pHi much more effectively than it increases [Ca2+]i, and thus also partially supports this contention. Cesium 15-18 glucose-6-phosphate isomerase Homo sapiens 100-103 3667622-10 1987 Treatment with Cs+ plus valinomycin (as an alternative method of membrane depolarization) increases pHi much more effectively than it increases [Ca2+]i, and thus also partially supports this contention. Valinomycin 24-35 glucose-6-phosphate isomerase Homo sapiens 100-103 3670401-3 1987 In all the excitable cells studied to date, the intracellular pH (pHi) is higher than would be predicted from a passive distribution of H+ ions, and consequently there is an outwardly directed electrochemical driving force for HCO3-. Bicarbonates 227-232 glucose-6-phosphate isomerase Homo sapiens 66-69 3670401-4 1987 In the presence of CO2/HCO3- therefore, activation of GABA-gated channels could give rise to a significant efflux of bicarbonate, leading to a fall in postsynaptic pHi. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 19-22 glucose-6-phosphate isomerase Homo sapiens 164-167 3670401-4 1987 In the presence of CO2/HCO3- therefore, activation of GABA-gated channels could give rise to a significant efflux of bicarbonate, leading to a fall in postsynaptic pHi. Bicarbonates 23-27 glucose-6-phosphate isomerase Homo sapiens 164-167 3670401-4 1987 In the presence of CO2/HCO3- therefore, activation of GABA-gated channels could give rise to a significant efflux of bicarbonate, leading to a fall in postsynaptic pHi. gamma-Aminobutyric Acid 54-58 glucose-6-phosphate isomerase Homo sapiens 164-167 3670401-4 1987 In the presence of CO2/HCO3- therefore, activation of GABA-gated channels could give rise to a significant efflux of bicarbonate, leading to a fall in postsynaptic pHi. Bicarbonates 117-128 glucose-6-phosphate isomerase Homo sapiens 164-167 3670401-5 1987 We have examined the influence of GABA on pHi in crayfish skeletal muscle and we find that in the presence of CO2, GABA induces a dramatic fall in pHi which is coupled to an alkalosis at the extracellular surface. gamma-Aminobutyric Acid 34-38 glucose-6-phosphate isomerase Homo sapiens 147-150 3670401-5 1987 We have examined the influence of GABA on pHi in crayfish skeletal muscle and we find that in the presence of CO2, GABA induces a dramatic fall in pHi which is coupled to an alkalosis at the extracellular surface. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 110-113 glucose-6-phosphate isomerase Homo sapiens 42-45 3670401-5 1987 We have examined the influence of GABA on pHi in crayfish skeletal muscle and we find that in the presence of CO2, GABA induces a dramatic fall in pHi which is coupled to an alkalosis at the extracellular surface. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 110-113 glucose-6-phosphate isomerase Homo sapiens 147-150 3670401-5 1987 We have examined the influence of GABA on pHi in crayfish skeletal muscle and we find that in the presence of CO2, GABA induces a dramatic fall in pHi which is coupled to an alkalosis at the extracellular surface. gamma-Aminobutyric Acid 115-119 glucose-6-phosphate isomerase Homo sapiens 42-45 3670401-5 1987 We have examined the influence of GABA on pHi in crayfish skeletal muscle and we find that in the presence of CO2, GABA induces a dramatic fall in pHi which is coupled to an alkalosis at the extracellular surface. gamma-Aminobutyric Acid 115-119 glucose-6-phosphate isomerase Homo sapiens 147-150 3670401-6 1987 This fall in pHi and the extracellular alkalosis are attributable to a GABA-activated, picrotoxin-sensitive HCO3--conductance. gamma-Aminobutyric Acid 71-75 glucose-6-phosphate isomerase Homo sapiens 13-16 3670401-6 1987 This fall in pHi and the extracellular alkalosis are attributable to a GABA-activated, picrotoxin-sensitive HCO3--conductance. Picrotoxin 87-97 glucose-6-phosphate isomerase Homo sapiens 13-16 3670401-6 1987 This fall in pHi and the extracellular alkalosis are attributable to a GABA-activated, picrotoxin-sensitive HCO3--conductance. Bicarbonates 108-112 glucose-6-phosphate isomerase Homo sapiens 13-16 3670401-7 1987 In view of the sensitivity of ion channels and intracellular ion concentrations to changes in pHi, a GABA-induced postsynaptic acidosis could prove to be important in the modulation of inhibitory transmission. gamma-Aminobutyric Acid 101-105 glucose-6-phosphate isomerase Homo sapiens 94-97 2821820-2 1987 By means of intracellular dialysis, we found that changes of intracellular pH (pHi), but not of extracellular pH, affected ouabain-sensitive Na+ efflux and K+ influx over the pH range of 6.0-8.6. Ouabain 123-130 glucose-6-phosphate isomerase Homo sapiens 79-82 2821820-2 1987 By means of intracellular dialysis, we found that changes of intracellular pH (pHi), but not of extracellular pH, affected ouabain-sensitive Na+ efflux and K+ influx over the pH range of 6.0-8.6. Ouabain 123-130 glucose-6-phosphate isomerase Homo sapiens 79-81 2821820-2 1987 By means of intracellular dialysis, we found that changes of intracellular pH (pHi), but not of extracellular pH, affected ouabain-sensitive Na+ efflux and K+ influx over the pH range of 6.0-8.6. Ouabain 123-130 glucose-6-phosphate isomerase Homo sapiens 79-81 2821820-4 1987 Variations away from this optimal pHi in either the acidic or alkaline direction resulted in a graded inhibition of both ouabain-sensitive fluxes. Ouabain 121-128 glucose-6-phosphate isomerase Homo sapiens 34-37 2821820-5 1987 The kinetic basis for the inhibitory effect of acidic pHi was examined by comparing the kinetic parameters of activation of ouabain-sensitive sodium efflux by intracellular Na+ (Na+i) and extracellular K+ (K+o) at normal pHi with those at acidic pHi. Sodium 142-148 glucose-6-phosphate isomerase Homo sapiens 54-57 3036868-4 1987 In both cell types, recovery of pHi from an NH+4-induced acid load follows an exponential time course and is entirely mediated by the amiloride-sensitive Na+/H+ exchanger in the plasma membrane. Amiloride 134-143 glucose-6-phosphate isomerase Homo sapiens 32-35 3653394-3 1987 Changes of pHi up to +/- 0.35 units, imposed by additions of NH4Cl, CO2 or nigericin, produced no shape change or aggregation and only insignificant changes in [Ca2+]i. Ammonium Chloride 61-66 glucose-6-phosphate isomerase Homo sapiens 11-14 3653394-3 1987 Changes of pHi up to +/- 0.35 units, imposed by additions of NH4Cl, CO2 or nigericin, produced no shape change or aggregation and only insignificant changes in [Ca2+]i. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 68-71 glucose-6-phosphate isomerase Homo sapiens 11-14 3653394-3 1987 Changes of pHi up to +/- 0.35 units, imposed by additions of NH4Cl, CO2 or nigericin, produced no shape change or aggregation and only insignificant changes in [Ca2+]i. Nigericin 75-84 glucose-6-phosphate isomerase Homo sapiens 11-14 3039678-4 1987 In contrast, acidification of the serosal solution to pH 4.5 by replacing the bicarbonate reduced pHi from 7.32 +/- 0.04 to 6.95 +/- 0.06 (p less than 0.001, n = 8). Bicarbonates 78-89 glucose-6-phosphate isomerase Homo sapiens 98-101 3039678-5 1987 Similarly, in tissues bathed with HEPES-buffered Ringer"s solution (pH 7.0), pHi was unaffected by reducing the mucosal solution pH to 4.5 with HCl but fell 0.21 +/- 0.05 pH units (p less than 0.01, n = 7) during acidification of the serosal solution to pH 6. HEPES 34-39 glucose-6-phosphate isomerase Homo sapiens 77-80 3036868-5 1987 Kinetic analysis indicates that the higher steady-state pHi in P19 EC cells is due to an alkaline shift in the pHi sensitivity of the Na+/H+ exchange rate, as compared to that in MES-1 cells. 2-(N-morpholino)ethanesulfonic acid 179-182 glucose-6-phosphate isomerase Homo sapiens 56-59 3036868-6 1987 The Na+/H+ exchanger of MES-1 cells is responsive to epidermal growth factor, platelet-derived growth factor, serum, phorbol esters, and diacylglycerol, as shown by a rapid amiloride-sensitive rise in pHi of 0.15-0.35 unit. 2-(N-morpholino)ethanesulfonic acid 24-27 glucose-6-phosphate isomerase Homo sapiens 201-204 3036868-6 1987 The Na+/H+ exchanger of MES-1 cells is responsive to epidermal growth factor, platelet-derived growth factor, serum, phorbol esters, and diacylglycerol, as shown by a rapid amiloride-sensitive rise in pHi of 0.15-0.35 unit. Phorbol Esters 117-131 glucose-6-phosphate isomerase Homo sapiens 201-204 3036868-6 1987 The Na+/H+ exchanger of MES-1 cells is responsive to epidermal growth factor, platelet-derived growth factor, serum, phorbol esters, and diacylglycerol, as shown by a rapid amiloride-sensitive rise in pHi of 0.15-0.35 unit. Diglycerides 137-151 glucose-6-phosphate isomerase Homo sapiens 201-204 3036868-6 1987 The Na+/H+ exchanger of MES-1 cells is responsive to epidermal growth factor, platelet-derived growth factor, serum, phorbol esters, and diacylglycerol, as shown by a rapid amiloride-sensitive rise in pHi of 0.15-0.35 unit. Amiloride 173-182 glucose-6-phosphate isomerase Homo sapiens 201-204 3605330-3 1987 In addition, DPC caused the following effects, all consistent with inhibition of apical membrane Cl(-)-HCO3- exchange: fall in intracellular Cl- activity (aCli), increase in intracellular pH (pHi), reduction of the changes in aCli and pHi produced by lowering mucosal solution [Cl-], and reduction of the change in pHi produced by lowering mucosal solution [HCO3-]. fenamic acid 13-16 glucose-6-phosphate isomerase Homo sapiens 192-195 3605330-3 1987 In addition, DPC caused the following effects, all consistent with inhibition of apical membrane Cl(-)-HCO3- exchange: fall in intracellular Cl- activity (aCli), increase in intracellular pH (pHi), reduction of the changes in aCli and pHi produced by lowering mucosal solution [Cl-], and reduction of the change in pHi produced by lowering mucosal solution [HCO3-]. fenamic acid 13-16 glucose-6-phosphate isomerase Homo sapiens 235-238 3605330-3 1987 In addition, DPC caused the following effects, all consistent with inhibition of apical membrane Cl(-)-HCO3- exchange: fall in intracellular Cl- activity (aCli), increase in intracellular pH (pHi), reduction of the changes in aCli and pHi produced by lowering mucosal solution [Cl-], and reduction of the change in pHi produced by lowering mucosal solution [HCO3-]. fenamic acid 13-16 glucose-6-phosphate isomerase Homo sapiens 235-238 3605330-6 1987 In addition, DPC had no effects on the rapid changes in apical membrane voltage elicited by altering mucosal [Cl-], but caused significant reductions of the slower, secondary voltage changes observed in response to changes in mucosal [Cl-], and the changes in aCli and pHi produced by lowering mucosal [Cl-]. fenamic acid 13-16 glucose-6-phosphate isomerase Homo sapiens 269-272 3153328-5 1987 In Case 2, PH I was diagnosed early because of the finding of increased renal echogenicity at 3 weeks of age; this patient had numerous episodes of stone formation despite continuous treatment with pyridoxine, but maintained renal function with normal serum creatinine levels at the age of 28 months. Pyridoxine 198-208 glucose-6-phosphate isomerase Homo sapiens 11-15 3153328-5 1987 In Case 2, PH I was diagnosed early because of the finding of increased renal echogenicity at 3 weeks of age; this patient had numerous episodes of stone formation despite continuous treatment with pyridoxine, but maintained renal function with normal serum creatinine levels at the age of 28 months. Creatinine 258-268 glucose-6-phosphate isomerase Homo sapiens 11-15 3039752-4 1987 Immunoprecipitation of 32P-labeled VZV-infected cells showed that the precursor-products of gpI are phosphorylated. Phosphorus-32 23-26 glucose-6-phosphate isomerase Homo sapiens 92-95 3473475-1 1987 The hexose transport system of a fibroblast mutant, DS7, unable to convert glucose 6-phosphate to fructose 6-phosphate ("the phosphoglucose isomerase mutant"), is subject to a specific down-regulation ("curb") evoked by only glucose or D-allose. Hexoses 4-10 glucose-6-phosphate isomerase Homo sapiens 125-149 3473475-1 1987 The hexose transport system of a fibroblast mutant, DS7, unable to convert glucose 6-phosphate to fructose 6-phosphate ("the phosphoglucose isomerase mutant"), is subject to a specific down-regulation ("curb") evoked by only glucose or D-allose. Glucose-6-Phosphate 75-94 glucose-6-phosphate isomerase Homo sapiens 125-149 3473475-1 1987 The hexose transport system of a fibroblast mutant, DS7, unable to convert glucose 6-phosphate to fructose 6-phosphate ("the phosphoglucose isomerase mutant"), is subject to a specific down-regulation ("curb") evoked by only glucose or D-allose. allose 236-244 glucose-6-phosphate isomerase Homo sapiens 125-149 3040449-1 1987 The effect of the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on cytoplasmic pH (pHi) and H+ extrusion was studied in the human monoblastic cell line U-937. Phorbol Esters 34-47 glucose-6-phosphate isomerase Homo sapiens 110-113 3040449-1 1987 The effect of the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on cytoplasmic pH (pHi) and H+ extrusion was studied in the human monoblastic cell line U-937. Tetradecanoylphorbol Acetate 48-84 glucose-6-phosphate isomerase Homo sapiens 110-113 3040449-1 1987 The effect of the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) on cytoplasmic pH (pHi) and H+ extrusion was studied in the human monoblastic cell line U-937. Tetradecanoylphorbol Acetate 86-89 glucose-6-phosphate isomerase Homo sapiens 110-113 3040449-2 1987 About 2 min after addition of TPA, pHi started to increase and reached a steady state 10-15 min later. Tetradecanoylphorbol Acetate 30-33 glucose-6-phosphate isomerase Homo sapiens 35-38 3040449-4 1987 The TPA-induced increase in pHi was independent of the presence of extracellular Na+. Tetradecanoylphorbol Acetate 4-7 glucose-6-phosphate isomerase Homo sapiens 28-31 3599651-4 1987 In the present studies, the pH-sensitive fluorescent dye, bis-(carboxyethyl)-carboxyfluorescein (BCECF) has been used to study pHi changes in suspensions of canine proximal tubule cells following acidification or alkalinization of the cytosol. bis-(carboxyethyl)-carboxyfluorescein 58-95 glucose-6-phosphate isomerase Homo sapiens 127-130 3621057-2 1987 The mean pHi of the glial cells was 6.87 +/- 0.13 (+/- SD, n = 27) in HEPES-buffered saline (pHo 7.4) and 7.18 +/- 0.19 (n = 13) in solutions buffered with 2% CO2- 11 mM HCO3-. Sodium Chloride 85-91 glucose-6-phosphate isomerase Homo sapiens 9-12 3621057-4 1987 To investigate pHi regulation, the pHi was decreased by exposure to CO2 or by adding and then removing NH4Cl. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 68-71 glucose-6-phosphate isomerase Homo sapiens 35-38 3621057-4 1987 To investigate pHi regulation, the pHi was decreased by exposure to CO2 or by adding and then removing NH4Cl. Ammonium Chloride 103-108 glucose-6-phosphate isomerase Homo sapiens 35-38 3621057-6 1987 The pHi recovery from acidification in neuropile glial cells in HEPES-buffered saline and CO2-HCO3- buffered saline was, however, blocked by removing external Na. hepes-buffered saline 64-85 glucose-6-phosphate isomerase Homo sapiens 4-7 3621057-7 1987 In HCO3(-)-free solutions the diuretic amiloride (2 mM) reduced the rate of pHi recovery. Amiloride 39-48 glucose-6-phosphate isomerase Homo sapiens 76-79 3621057-8 1987 In the presence of HCO3-, the rate of acid efflux was stimulated; the stilbene 4-acetamido-4"-isothiocyanatostilbene-2,3"-disulfonic acid (SITS; 0.5 mM) slowed pHi recovery. Bicarbonates 19-23 glucose-6-phosphate isomerase Homo sapiens 160-163 3621057-8 1987 In the presence of HCO3-, the rate of acid efflux was stimulated; the stilbene 4-acetamido-4"-isothiocyanatostilbene-2,3"-disulfonic acid (SITS; 0.5 mM) slowed pHi recovery. stilbene 4-acetamido-4"-isothiocyanatostilbene-2,3"-disulfonic acid 70-137 glucose-6-phosphate isomerase Homo sapiens 160-163 3621057-8 1987 In the presence of HCO3-, the rate of acid efflux was stimulated; the stilbene 4-acetamido-4"-isothiocyanatostilbene-2,3"-disulfonic acid (SITS; 0.5 mM) slowed pHi recovery. 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 139-143 glucose-6-phosphate isomerase Homo sapiens 160-163 3621057-9 1987 In HEPES buffered and CO2-HCO3- buffered solutions pHi regulation in neurones was inhibited by removing external Na. HEPES 3-8 glucose-6-phosphate isomerase Homo sapiens 51-54 3621057-9 1987 In HEPES buffered and CO2-HCO3- buffered solutions pHi regulation in neurones was inhibited by removing external Na. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 22-25 glucose-6-phosphate isomerase Homo sapiens 51-54 3621057-9 1987 In HEPES buffered and CO2-HCO3- buffered solutions pHi regulation in neurones was inhibited by removing external Na. Bicarbonates 26-30 glucose-6-phosphate isomerase Homo sapiens 51-54 3621057-10 1987 In HCO3(-)-free solutions amiloride reduced the rate of pHi recovery considerably. Amiloride 26-35 glucose-6-phosphate isomerase Homo sapiens 56-59 3621057-12 1987 We conclude that leech glial cells and neurones have two mechanisms of pHi regulation, one being Na+-H+ exchange and the other Na+ and HCO3- dependent. Bicarbonates 135-139 glucose-6-phosphate isomerase Homo sapiens 71-74 3621058-4 1987 The recovery of pHi was blocked by 1-2 mM amiloride. Amiloride 42-51 glucose-6-phosphate isomerase Homo sapiens 16-19 3621058-8 1987 Transition from HEPES to HCO3(-)-buffered media increased the rate of acid extrusion during recovery of pHi. HEPES 16-21 glucose-6-phosphate isomerase Homo sapiens 104-107 3621058-8 1987 Transition from HEPES to HCO3(-)-buffered media increased the rate of acid extrusion during recovery of pHi. hco3(-)-buffered media 25-47 glucose-6-phosphate isomerase Homo sapiens 104-107 3621058-10 1987 Following inhibition of acid extrusion by amiloride, transition to HCO3- media restored pHi recovery. Bicarbonates 67-71 glucose-6-phosphate isomerase Homo sapiens 88-91 3472234-9 1987 Potassium transport is accelerated at low pHi, but in a manner consistent with its inherent voltage sensitivity and changes in Vm resulting from an increased rate of H+ extrusion by the pump. Potassium 0-9 glucose-6-phosphate isomerase Homo sapiens 42-45 3599651-4 1987 In the present studies, the pH-sensitive fluorescent dye, bis-(carboxyethyl)-carboxyfluorescein (BCECF) has been used to study pHi changes in suspensions of canine proximal tubule cells following acidification or alkalinization of the cytosol. bcecf 97-102 glucose-6-phosphate isomerase Homo sapiens 127-130 3599651-6 1987 Following removal of nigericin, pHi returned to basal levels (pHi = 7.1) when the cells were resuspended in a buffer containing 100 mM Na+. Nigericin 21-30 glucose-6-phosphate isomerase Homo sapiens 32-35 3599651-6 1987 Following removal of nigericin, pHi returned to basal levels (pHi = 7.1) when the cells were resuspended in a buffer containing 100 mM Na+. Nigericin 21-30 glucose-6-phosphate isomerase Homo sapiens 62-65 3599651-9 1987 When cell membrane potential was monitored in these experiments using the potential-sensitive fluorescent dye, bis-(1,3-dibutylbarbiturate) trimethine oxonol, the increase in pHi seen in the presence of Na+ was found to be electroneutral, whereas when that occurred in the presence of Na+, amiloride and HCO3-/CO2 was associated with membrane hyperpolarization. bis(1,3-dibutylbarbiturate)trimethine oxonol 111-157 glucose-6-phosphate isomerase Homo sapiens 175-178 3599651-9 1987 When cell membrane potential was monitored in these experiments using the potential-sensitive fluorescent dye, bis-(1,3-dibutylbarbiturate) trimethine oxonol, the increase in pHi seen in the presence of Na+ was found to be electroneutral, whereas when that occurred in the presence of Na+, amiloride and HCO3-/CO2 was associated with membrane hyperpolarization. Amiloride 290-299 glucose-6-phosphate isomerase Homo sapiens 175-178 3599651-9 1987 When cell membrane potential was monitored in these experiments using the potential-sensitive fluorescent dye, bis-(1,3-dibutylbarbiturate) trimethine oxonol, the increase in pHi seen in the presence of Na+ was found to be electroneutral, whereas when that occurred in the presence of Na+, amiloride and HCO3-/CO2 was associated with membrane hyperpolarization. Bicarbonates 304-309 glucose-6-phosphate isomerase Homo sapiens 175-178 3599651-9 1987 When cell membrane potential was monitored in these experiments using the potential-sensitive fluorescent dye, bis-(1,3-dibutylbarbiturate) trimethine oxonol, the increase in pHi seen in the presence of Na+ was found to be electroneutral, whereas when that occurred in the presence of Na+, amiloride and HCO3-/CO2 was associated with membrane hyperpolarization. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 310-313 glucose-6-phosphate isomerase Homo sapiens 175-178 3029782-0 1987 Aldosterone activates Na+/H+ exchange and raises cytoplasmic pH in target cells of the amphibian kidney. Aldosterone 0-11 glucose-6-phosphate isomerase Homo sapiens 61-63 3298291-1 1987 The dansyl derivative of glucosyl galactosyl hydroxylysine (GGH) was separated into two components, as GP-I (monodansyl GGH) and GP-II (didansyl GGH) by paper chromatography. glucosylgalactosylhydroxylysine 60-63 glucose-6-phosphate isomerase Homo sapiens 103-107 3815351-2 1987 Cells may survive conditions of acid pHe because antiports in their membrane exchange Na+ for H+, or HCO3- for Cl-, and thus regulate the intracellular pH (pHi). acid phe 32-40 glucose-6-phosphate isomerase Homo sapiens 156-159 3815351-2 1987 Cells may survive conditions of acid pHe because antiports in their membrane exchange Na+ for H+, or HCO3- for Cl-, and thus regulate the intracellular pH (pHi). Bicarbonates 101-105 glucose-6-phosphate isomerase Homo sapiens 156-159 3815351-5 1987 Amiloride and 4,4"-diisothiocyanostilbene 2,2-disulfonic acid, inhibitors of the Na+/H+ and HCO3-/Cl- exchangers, respectively, decreased pHi in the presence of nigericin at low pHe. Amiloride 0-9 glucose-6-phosphate isomerase Homo sapiens 138-141 3815351-5 1987 Amiloride and 4,4"-diisothiocyanostilbene 2,2-disulfonic acid, inhibitors of the Na+/H+ and HCO3-/Cl- exchangers, respectively, decreased pHi in the presence of nigericin at low pHe. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 14-61 glucose-6-phosphate isomerase Homo sapiens 138-141 3815351-5 1987 Amiloride and 4,4"-diisothiocyanostilbene 2,2-disulfonic acid, inhibitors of the Na+/H+ and HCO3-/Cl- exchangers, respectively, decreased pHi in the presence of nigericin at low pHe. Nigericin 161-170 glucose-6-phosphate isomerase Homo sapiens 138-141 3815351-5 1987 Amiloride and 4,4"-diisothiocyanostilbene 2,2-disulfonic acid, inhibitors of the Na+/H+ and HCO3-/Cl- exchangers, respectively, decreased pHi in the presence of nigericin at low pHe. Phenylalanine 178-181 glucose-6-phosphate isomerase Homo sapiens 138-141 3029782-5 1987 The diuretic drug amiloride blocked pHi recovery. Amiloride 18-27 glucose-6-phosphate isomerase Homo sapiens 36-39 2436344-7 1987 An important difference to all other solutions was the observation made under the St. Thomas solution with procaine, that after recovery to normal values pHi decreased between the 2.-5. minute to values of 6.39-6.48 when the preparations were superfused with Tyrode"s solution again. Procaine 107-115 glucose-6-phosphate isomerase Homo sapiens 154-157 3808046-2 1987 Several mechanisms for pHi regulation in different tissues have been found, such as direct proton pumping, Na/H exchange, Cl/HCO3 exchange, NaHCO3 cotransport, and Na/H/Cl/HCO3 obligatorily linked. Bicarbonates 125-129 glucose-6-phosphate isomerase Homo sapiens 23-26 3808046-2 1987 Several mechanisms for pHi regulation in different tissues have been found, such as direct proton pumping, Na/H exchange, Cl/HCO3 exchange, NaHCO3 cotransport, and Na/H/Cl/HCO3 obligatorily linked. Sodium Bicarbonate 140-146 glucose-6-phosphate isomerase Homo sapiens 23-26 3808046-2 1987 Several mechanisms for pHi regulation in different tissues have been found, such as direct proton pumping, Na/H exchange, Cl/HCO3 exchange, NaHCO3 cotransport, and Na/H/Cl/HCO3 obligatorily linked. Bicarbonates 142-146 glucose-6-phosphate isomerase Homo sapiens 23-26 3808046-6 1987 CCs become markedly more acidic upon removal of external Na (Nao), but pHi is restored rapidly on return to normal Nao, with or without Cl. 10-N-nonylacridinium orange 115-118 glucose-6-phosphate isomerase Homo sapiens 71-74 3808046-8 1987 Conversely, OCs become strongly more alkaline on removal of external Cl (Clo), pHi being restored when Clo is replaced with or without Na, whereas CCs are relatively insensitive to Clo. clo 103-106 glucose-6-phosphate isomerase Homo sapiens 79-82 3808046-8 1987 Conversely, OCs become strongly more alkaline on removal of external Cl (Clo), pHi being restored when Clo is replaced with or without Na, whereas CCs are relatively insensitive to Clo. clo 103-106 glucose-6-phosphate isomerase Homo sapiens 79-82 3026850-3 1987 Blocking Na+/H+ exchange by removal of extracellular Na+ or by ethylisopropylamiloride (EIPA) inhibited Ca2+ mobilization induced by 0.2 U/ml thrombin, whereas increasing pHi by NH4Cl enhanced the thrombin-induced increase in cytosolic free Ca2+. ethylisopropylamiloride 88-92 glucose-6-phosphate isomerase Homo sapiens 171-174 3491633-2 1986 The extracellular pH (pHe) of HEPES buffered Ringer"s solution influenced pHi, but change in pHi developed slowly. Phenylalanine 22-25 glucose-6-phosphate isomerase Homo sapiens 74-77 3551797-10 1987 They exhibit Na+-dependent and Cl(-)-dependent changes of pHi that are consistent with the presence of both Na+-H+ and Cl(-)-HCO3 exchangers. Bicarbonates 125-129 glucose-6-phosphate isomerase Homo sapiens 58-61 3566711-2 1987 Stimulation of platelets by thrombin or 12-O-tetradecanoylphorbol 13-acetate increased pHi by about 0.11 pH unit above the resting value. Tetradecanoylphorbol Acetate 40-76 glucose-6-phosphate isomerase Homo sapiens 87-90 3566711-3 1987 This increase in pHi depended on the presence of external Na+ and was inhibited by ethylisopropylamiloride. ethylisopropylamiloride 83-106 glucose-6-phosphate isomerase Homo sapiens 17-20 3802500-3 1987 This acidic pHi may be correlated with the increased concentration of ATP in erythrocytes in uremia, which is partly corrected by these two types of hemodialysis. Adenosine Triphosphate 70-73 glucose-6-phosphate isomerase Homo sapiens 12-15 3559479-3 1987 Lysis of the CSF leukocytes with Triton X-100 resulted in a substantial increase of phosphohexose isomerase activity. Octoxynol 33-45 glucose-6-phosphate isomerase Homo sapiens 84-107 3114472-2 1986 We examined, in frog semitendinosus muscle, the effect of calcium release, induced by depolarization or caffeine, on intracellular pH (pHi) recovery from an acid load applied at least 40 min later. Calcium 58-65 glucose-6-phosphate isomerase Homo sapiens 135-138 3114472-6 1986 In fibres depolarized by 50 mM-K, constant [K] X [Cl] in the presence of 1 mM-tetracaine (which blocks Ca release), the rate of pHi recovery from 5% CO2-induced acidification was 0.15 +/- 0.02 delta pHi h-1 (n = 7), whereas in depolarized fibres that had never been exposed to the drug, the rate of recovery was 0.27 +/- 0.01 delta pHi h-1 (n = 5). Tetracaine 77-88 glucose-6-phosphate isomerase Homo sapiens 128-131 3114472-6 1986 In fibres depolarized by 50 mM-K, constant [K] X [Cl] in the presence of 1 mM-tetracaine (which blocks Ca release), the rate of pHi recovery from 5% CO2-induced acidification was 0.15 +/- 0.02 delta pHi h-1 (n = 7), whereas in depolarized fibres that had never been exposed to the drug, the rate of recovery was 0.27 +/- 0.01 delta pHi h-1 (n = 5). Tetracaine 77-88 glucose-6-phosphate isomerase Homo sapiens 199-202 3114472-6 1986 In fibres depolarized by 50 mM-K, constant [K] X [Cl] in the presence of 1 mM-tetracaine (which blocks Ca release), the rate of pHi recovery from 5% CO2-induced acidification was 0.15 +/- 0.02 delta pHi h-1 (n = 7), whereas in depolarized fibres that had never been exposed to the drug, the rate of recovery was 0.27 +/- 0.01 delta pHi h-1 (n = 5). Tetracaine 77-88 glucose-6-phosphate isomerase Homo sapiens 199-202 3114472-6 1986 In fibres depolarized by 50 mM-K, constant [K] X [Cl] in the presence of 1 mM-tetracaine (which blocks Ca release), the rate of pHi recovery from 5% CO2-induced acidification was 0.15 +/- 0.02 delta pHi h-1 (n = 7), whereas in depolarized fibres that had never been exposed to the drug, the rate of recovery was 0.27 +/- 0.01 delta pHi h-1 (n = 5). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 149-152 glucose-6-phosphate isomerase Homo sapiens 128-131 3114472-7 1986 Yet, when Ca release was not blocked and the depolarized fibres were exposed to tetracaine shortly before CO2 exposure, a similar slow rate of 0.14 +/- 0.03 delta pHi h-1 (n = 7) was observed. Tetracaine 80-90 glucose-6-phosphate isomerase Homo sapiens 163-166 3114472-15 1986 Tetracaine reversibly inhibits pHi recovery, but this inhibition is not due to its blocking of Ca release. Tetracaine 0-10 glucose-6-phosphate isomerase Homo sapiens 31-34 3114472-17 1986 Recovery from CO2-induced acidification of fibres depolarized to -21 mV in 50 mM-K, constant Cl was halved, from 0.31 +/- 0.04 delta pHi h-1 (n = 10) to 0.15 +/- 0.01 delta pHi h-1 (n = 13), when external Ca was raised from 4 to 10 mM. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 14-17 glucose-6-phosphate isomerase Homo sapiens 133-136 3114472-17 1986 Recovery from CO2-induced acidification of fibres depolarized to -21 mV in 50 mM-K, constant Cl was halved, from 0.31 +/- 0.04 delta pHi h-1 (n = 10) to 0.15 +/- 0.01 delta pHi h-1 (n = 13), when external Ca was raised from 4 to 10 mM. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 14-17 glucose-6-phosphate isomerase Homo sapiens 173-176 2946710-7 1986 Peak LH responses to nafarelin decreased by about 70% (Gps I and II) and 95% (Gp III). Luteinizing Hormone 5-7 glucose-6-phosphate isomerase Homo sapiens 55-67 2946710-9 1986 Serum estradiol levels increased acutely in Gps I and II, but not in Gp III, in response to each dose of nafarelin. Estradiol 6-15 glucose-6-phosphate isomerase Homo sapiens 44-56 3024158-5 1986 The enzyme catalyzed the transfer of 32Pi from [gamma-32P]ATP to gpI but not to gpII and gpIII. 32pi 37-41 glucose-6-phosphate isomerase Homo sapiens 65-68 3024158-5 1986 The enzyme catalyzed the transfer of 32Pi from [gamma-32P]ATP to gpI but not to gpII and gpIII. [gamma-32p]atp 47-61 glucose-6-phosphate isomerase Homo sapiens 65-68 3491633-2 1986 The extracellular pH (pHe) of HEPES buffered Ringer"s solution influenced pHi, but change in pHi developed slowly. Phenylalanine 22-25 glucose-6-phosphate isomerase Homo sapiens 93-96 3491633-2 1986 The extracellular pH (pHe) of HEPES buffered Ringer"s solution influenced pHi, but change in pHi developed slowly. HEPES 30-35 glucose-6-phosphate isomerase Homo sapiens 74-77 3491633-2 1986 The extracellular pH (pHe) of HEPES buffered Ringer"s solution influenced pHi, but change in pHi developed slowly. HEPES 30-35 glucose-6-phosphate isomerase Homo sapiens 93-96 3491633-3 1986 Addition or removal of CO2 or NH3 from the extracellular solution caused a rapid change in pHi. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 23-26 glucose-6-phosphate isomerase Homo sapiens 91-94 3491633-3 1986 Addition or removal of CO2 or NH3 from the extracellular solution caused a rapid change in pHi. Ammonia 30-33 glucose-6-phosphate isomerase Homo sapiens 91-94 3491633-5 1986 For pHi values above approximately 7.0, the observed buffer power was greater than that expected from the values in the literature for the histidine content of intracellular proteins, carnosine and inorganic phosphate in the sarcoplasm. Histidine 139-148 glucose-6-phosphate isomerase Homo sapiens 4-7 3020026-2 1986 Intracellular pH (pHi) of human platelets was measured with the fluorescent dye 2",7"-bis(carboxyethyl)5,6-carboxyfluorescein under various conditions. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 80-125 glucose-6-phosphate isomerase Homo sapiens 18-21 3016497-2 1986 Intracellular pH (pHi) changes were measured from the equilibrium distribution of 14C-labeled 5,5-dimethyloxazolidine-2,4-dione (DMO). Carbon-14 82-85 glucose-6-phosphate isomerase Homo sapiens 18-21 3020026-5 1986 In Na+-free medium, or in presence of the Na+/H+ antiport inhibitors, 5-(N,N-dimethyl)amiloride (DMA) or 5-(N-ethyl-N-isopropyl)amiloride (EIPA), thrombin caused a greater fall of pHi (0.22-0.26 units) that was sustained. 5-dimethylamiloride 70-95 glucose-6-phosphate isomerase Homo sapiens 180-183 3020026-7 1986 Ca2+ ionophores (ionomycin, A23187) decreased platelet pHi by 0.02-0.15 units, but without an increase of pHi comparable to that following thrombin; DMA and EIPA enhanced the fall of pHi (0.14-0.33 units). Ionomycin 17-26 glucose-6-phosphate isomerase Homo sapiens 55-58 3020026-7 1986 Ca2+ ionophores (ionomycin, A23187) decreased platelet pHi by 0.02-0.15 units, but without an increase of pHi comparable to that following thrombin; DMA and EIPA enhanced the fall of pHi (0.14-0.33 units). Calcimycin 28-34 glucose-6-phosphate isomerase Homo sapiens 55-58 3017962-15 1986 It is concluded that in nominally bicarbonate-free saline, the amiloride-sensitive Na+/H+ antiport is the predominant mechanism of pHi regulation at acidic pHi, while being relatively inactive at physiological values of pHi. Sodium Chloride 51-57 glucose-6-phosphate isomerase Homo sapiens 131-134 3017962-15 1986 It is concluded that in nominally bicarbonate-free saline, the amiloride-sensitive Na+/H+ antiport is the predominant mechanism of pHi regulation at acidic pHi, while being relatively inactive at physiological values of pHi. Sodium Chloride 51-57 glucose-6-phosphate isomerase Homo sapiens 156-159 3017962-15 1986 It is concluded that in nominally bicarbonate-free saline, the amiloride-sensitive Na+/H+ antiport is the predominant mechanism of pHi regulation at acidic pHi, while being relatively inactive at physiological values of pHi. Sodium Chloride 51-57 glucose-6-phosphate isomerase Homo sapiens 156-159 3017962-16 1986 In bicarbonate saline, two other mechanisms effect pHi regulation: a DIDS-sensitive Na+-HCO3- symport, which contributes to cytoplasmic alkalinization, and a DIDS-sensitive Cl-/HCO3- exchange, which is apparently independent of Na+. Bicarbonates 3-14 glucose-6-phosphate isomerase Homo sapiens 51-54 3017962-16 1986 In bicarbonate saline, two other mechanisms effect pHi regulation: a DIDS-sensitive Na+-HCO3- symport, which contributes to cytoplasmic alkalinization, and a DIDS-sensitive Cl-/HCO3- exchange, which is apparently independent of Na+. Sodium Chloride 15-21 glucose-6-phosphate isomerase Homo sapiens 51-54 3017962-16 1986 In bicarbonate saline, two other mechanisms effect pHi regulation: a DIDS-sensitive Na+-HCO3- symport, which contributes to cytoplasmic alkalinization, and a DIDS-sensitive Cl-/HCO3- exchange, which is apparently independent of Na+. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 69-73 glucose-6-phosphate isomerase Homo sapiens 51-54 3017962-16 1986 In bicarbonate saline, two other mechanisms effect pHi regulation: a DIDS-sensitive Na+-HCO3- symport, which contributes to cytoplasmic alkalinization, and a DIDS-sensitive Cl-/HCO3- exchange, which is apparently independent of Na+. Bicarbonates 88-92 glucose-6-phosphate isomerase Homo sapiens 51-54 3017962-16 1986 In bicarbonate saline, two other mechanisms effect pHi regulation: a DIDS-sensitive Na+-HCO3- symport, which contributes to cytoplasmic alkalinization, and a DIDS-sensitive Cl-/HCO3- exchange, which is apparently independent of Na+. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 158-162 glucose-6-phosphate isomerase Homo sapiens 51-54 3017962-16 1986 In bicarbonate saline, two other mechanisms effect pHi regulation: a DIDS-sensitive Na+-HCO3- symport, which contributes to cytoplasmic alkalinization, and a DIDS-sensitive Cl-/HCO3- exchange, which is apparently independent of Na+. Bicarbonates 177-181 glucose-6-phosphate isomerase Homo sapiens 51-54 2428906-5 1986 The rise in pHi has a permissive effect on DNA synthesis and is mediated by an otherwise quiescent Na+/H+ exchange mechanism in the plasma membrane, which is turned on by protein kinase C, the cellular receptor for phorbol esters. Phorbol Esters 215-229 glucose-6-phosphate isomerase Homo sapiens 12-15 3793878-6 1986 Addition of TPA caused no change in [Ca2+]i but a biphasic change in pHi. Tetradecanoylphorbol Acetate 12-15 glucose-6-phosphate isomerase Homo sapiens 69-72 3017962-4 1986 After an acid load, the cells regulate pHi in the absence of HCO3- by a Na+ (or Li+)-dependent, amiloride-inhibitable mechanism (indicative of Na+/H+ antiport). Amiloride 96-105 glucose-6-phosphate isomerase Homo sapiens 39-42 3017962-11 1986 When HCO3- was lowered from 46 to 10 mM at constant pCO2 (5%), pHi dropped by a DIDS-sensitive mechanism. Bicarbonates 5-9 glucose-6-phosphate isomerase Homo sapiens 63-66 3017962-11 1986 When HCO3- was lowered from 46 to 10 mM at constant pCO2 (5%), pHi dropped by a DIDS-sensitive mechanism. pco2 52-56 glucose-6-phosphate isomerase Homo sapiens 63-66 3017962-11 1986 When HCO3- was lowered from 46 to 10 mM at constant pCO2 (5%), pHi dropped by a DIDS-sensitive mechanism. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 80-84 glucose-6-phosphate isomerase Homo sapiens 63-66 3017962-15 1986 It is concluded that in nominally bicarbonate-free saline, the amiloride-sensitive Na+/H+ antiport is the predominant mechanism of pHi regulation at acidic pHi, while being relatively inactive at physiological values of pHi. Bicarbonates 34-45 glucose-6-phosphate isomerase Homo sapiens 131-134 3017962-15 1986 It is concluded that in nominally bicarbonate-free saline, the amiloride-sensitive Na+/H+ antiport is the predominant mechanism of pHi regulation at acidic pHi, while being relatively inactive at physiological values of pHi. Bicarbonates 34-45 glucose-6-phosphate isomerase Homo sapiens 156-159 3017962-15 1986 It is concluded that in nominally bicarbonate-free saline, the amiloride-sensitive Na+/H+ antiport is the predominant mechanism of pHi regulation at acidic pHi, while being relatively inactive at physiological values of pHi. Bicarbonates 34-45 glucose-6-phosphate isomerase Homo sapiens 156-159 3016497-2 1986 Intracellular pH (pHi) changes were measured from the equilibrium distribution of 14C-labeled 5,5-dimethyloxazolidine-2,4-dione (DMO). Dimethadione 94-127 glucose-6-phosphate isomerase Homo sapiens 18-21 3016497-2 1986 Intracellular pH (pHi) changes were measured from the equilibrium distribution of 14C-labeled 5,5-dimethyloxazolidine-2,4-dione (DMO). Dimethadione 129-132 glucose-6-phosphate isomerase Homo sapiens 18-21 3016497-3 1986 Exposure of cells to 10 nm N-formyl-methionyl-leucyl-phenylalanine (FMLP) caused activation of Na+/H+ exchange: in 140 mM Na+ medium (extracellular pH 7.40), the pHi rose from a resting value of approximately 7.25 to reach a new steady state of approximately 7.75 by 10-15 min. N-Formylmethionine Leucyl-Phenylalanine 68-72 glucose-6-phosphate isomerase Homo sapiens 162-165 3016497-5 1986 The structure-activity relationships in the amiloride series were characterized by testing the effect of these compounds on the DMO-derived pHi changes and on the FMLP-stimulated rate of 22Na+ efflux from the cells. Amiloride 44-53 glucose-6-phosphate isomerase Homo sapiens 140-143 3016497-12 1986 The development of potent derivatives of amiloride should provide powerful tools for assessing the role of FMLP-activated Na+/H+ exchange and the resultant pHi transients on stimulated neutrophil functions. Amiloride 41-50 glucose-6-phosphate isomerase Homo sapiens 156-159 3953781-7 1986 The rate of amiloride-sensitive H+ efflux could be calculated from the rate of change of pHi, using a buffering power of 28 mmol X l-1 X pH unit-1, determined by titration with NH+4 or propionate-. Amiloride 12-21 glucose-6-phosphate isomerase Homo sapiens 89-92 3014886-8 1986 N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline recoupled partially uncoupled axons by decreasing Rj and prevented subsequent uncoupling of the junction by low pHi. EEDQ 0-46 glucose-6-phosphate isomerase Homo sapiens 159-162 3085092-4 1986 Certain drugs irreversibly (glutaraldehyde, 1-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline) or reversibly (retinoic acid) abolish dependence of gj on pHi without appreciably affecting kinetic properties of voltage dependence or the shape of the steady-state Vj-gj relation. Glutaral 28-42 glucose-6-phosphate isomerase Homo sapiens 150-153 3085092-4 1986 Certain drugs irreversibly (glutaraldehyde, 1-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline) or reversibly (retinoic acid) abolish dependence of gj on pHi without appreciably affecting kinetic properties of voltage dependence or the shape of the steady-state Vj-gj relation. EEDQ 44-90 glucose-6-phosphate isomerase Homo sapiens 150-153 3085092-4 1986 Certain drugs irreversibly (glutaraldehyde, 1-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline) or reversibly (retinoic acid) abolish dependence of gj on pHi without appreciably affecting kinetic properties of voltage dependence or the shape of the steady-state Vj-gj relation. Tretinoin 107-120 glucose-6-phosphate isomerase Homo sapiens 150-153 3007505-5 1986 On the other hand, reduction of the intracellular pH (pHi) to approximately 6.0-6.2 results in a similar effective activation of Na+/H+ exchange in both control and ATP-depleted cells. Adenosine Triphosphate 165-168 glucose-6-phosphate isomerase Homo sapiens 54-57 3007505-6 1986 Na+/H+ exchange activity in ATP-depleted cells that either have or have not been loaded with Na+ shows a steep dependency on pHi, being essentially abolished above pHi of 6.4-6.5, whereas control cells show a considerable activity also at more alkaline pHi values. Adenosine Triphosphate 28-31 glucose-6-phosphate isomerase Homo sapiens 125-128 3007505-6 1986 Na+/H+ exchange activity in ATP-depleted cells that either have or have not been loaded with Na+ shows a steep dependency on pHi, being essentially abolished above pHi of 6.4-6.5, whereas control cells show a considerable activity also at more alkaline pHi values. Adenosine Triphosphate 28-31 glucose-6-phosphate isomerase Homo sapiens 164-167 3007505-6 1986 Na+/H+ exchange activity in ATP-depleted cells that either have or have not been loaded with Na+ shows a steep dependency on pHi, being essentially abolished above pHi of 6.4-6.5, whereas control cells show a considerable activity also at more alkaline pHi values. Adenosine Triphosphate 28-31 glucose-6-phosphate isomerase Homo sapiens 164-167 3014886-3 1986 When applied to well-coupled axons, the sulfhydryl group reagents N-ethylmaleimide (NEM) and diamide uncoupled the segments; junctional resistance (Rj) was increased without changing membrane resistance or axoplasmic pH (pHi). Ethylmaleimide 66-82 glucose-6-phosphate isomerase Homo sapiens 221-224 3014886-3 1986 When applied to well-coupled axons, the sulfhydryl group reagents N-ethylmaleimide (NEM) and diamide uncoupled the segments; junctional resistance (Rj) was increased without changing membrane resistance or axoplasmic pH (pHi). Ethylmaleimide 84-87 glucose-6-phosphate isomerase Homo sapiens 221-224 3014886-3 1986 When applied to well-coupled axons, the sulfhydryl group reagents N-ethylmaleimide (NEM) and diamide uncoupled the segments; junctional resistance (Rj) was increased without changing membrane resistance or axoplasmic pH (pHi). Diamide 93-100 glucose-6-phosphate isomerase Homo sapiens 221-224 3719111-8 1986 Only GPI Homburg showed an altered electrophoretic mobility and an increased affinity for fructose-6-phosphate. fructose-6-phosphate 90-110 glucose-6-phosphate isomerase Homo sapiens 5-8 3488180-6 1986 The fluorescence of DCH is so strongly pH dependent that there were practical difficulties in its use over a wide pH range; however, pHi measurements are possible between pH 6.0 and pH 7.5 using either DCH or BCECF. dicyclohexylamine 20-23 glucose-6-phosphate isomerase Homo sapiens 133-136 3488180-6 1986 The fluorescence of DCH is so strongly pH dependent that there were practical difficulties in its use over a wide pH range; however, pHi measurements are possible between pH 6.0 and pH 7.5 using either DCH or BCECF. dicyclohexylamine 202-205 glucose-6-phosphate isomerase Homo sapiens 133-136 3488180-6 1986 The fluorescence of DCH is so strongly pH dependent that there were practical difficulties in its use over a wide pH range; however, pHi measurements are possible between pH 6.0 and pH 7.5 using either DCH or BCECF. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 209-214 glucose-6-phosphate isomerase Homo sapiens 133-136 3091840-5 1986 Although the presence of either substrate did not affect the response to hypercapnia, the addition of pyruvate was accompanied by a significant change in pHi. Pyruvic Acid 102-110 glucose-6-phosphate isomerase Homo sapiens 154-157 3091840-6 1986 Specifically, there was a monotonic decrease in pHi comparable to that observed when PCO2 is increased from 5% to 10% (delta OD = -0.018 +/- 0.002 CO2; delta OD = -0.020 +/- 0.002 PYR, respectively). pco2 85-89 glucose-6-phosphate isomerase Homo sapiens 48-51 3091840-6 1986 Specifically, there was a monotonic decrease in pHi comparable to that observed when PCO2 is increased from 5% to 10% (delta OD = -0.018 +/- 0.002 CO2; delta OD = -0.020 +/- 0.002 PYR, respectively). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 86-89 glucose-6-phosphate isomerase Homo sapiens 48-51 3091840-13 1986 However, with theophylline present, the addition of pyruvate was accompanied by an increase in pHi (delta OD = + 0.005 +/- 0.001). Theophylline 14-26 glucose-6-phosphate isomerase Homo sapiens 95-98 3091840-13 1986 However, with theophylline present, the addition of pyruvate was accompanied by an increase in pHi (delta OD = + 0.005 +/- 0.001). Pyruvic Acid 52-60 glucose-6-phosphate isomerase Homo sapiens 95-98 3091840-18 1986 The data suggest that pyruvate affects myocardial function by altering pHi, and this effect is not due to an increase in lactate. Pyruvic Acid 22-30 glucose-6-phosphate isomerase Homo sapiens 71-74 3013683-4 1986 Using nigericin/K+ to clamp pHi we demonstrated that the acidification accounts for the inhibition of O2 uptake. nigericin/k+ 6-18 glucose-6-phosphate isomerase Homo sapiens 28-31 3013683-4 1986 Using nigericin/K+ to clamp pHi we demonstrated that the acidification accounts for the inhibition of O2 uptake. Oxygen 102-104 glucose-6-phosphate isomerase Homo sapiens 28-31 3953781-7 1986 The rate of amiloride-sensitive H+ efflux could be calculated from the rate of change of pHi, using a buffering power of 28 mmol X l-1 X pH unit-1, determined by titration with NH+4 or propionate-. Propionates 185-195 glucose-6-phosphate isomerase Homo sapiens 89-92 3817608-1 1986 A 31P NMR method based on pH dependent variation of the chemical-shift-difference between the resonances of orthophosphate and methylphosphonate was used to measure simultaneously intracellular pH (pHi) and extracellular pH (pHc) during long term storage of erythrocytes; pH was determined at both 4 degrees C and 37 degrees C. An equation describing the equilibrium distribution of membrane-permeant ions was derived by consideration of the electrochemical and osmotic constraints in the RBC suspension. ET bromodomain inhibitor 2-5 glucose-6-phosphate isomerase Homo sapiens 198-201 3754753-6 1986 During monitored evaluation of arachidonic acid metabolites one patient with spontaneous angina demonstrated a gradual lowering of the 6-keto-PGI alpha, it being minimal by the beginning of the episode; TxB2 level increased more rapidly. Arachidonic Acid 31-47 glucose-6-phosphate isomerase Homo sapiens 142-145 3084168-8 1986 In hypertensives, only PgI was decreased, with increase after NTG, at rest. Nitroglycerin 62-65 glucose-6-phosphate isomerase Homo sapiens 23-26 3084168-9 1986 Thus, PgI is the only humoral indicator participating in blood pressure lowering induced by NTG. Nitroglycerin 92-95 glucose-6-phosphate isomerase Homo sapiens 6-9 3817608-1 1986 A 31P NMR method based on pH dependent variation of the chemical-shift-difference between the resonances of orthophosphate and methylphosphonate was used to measure simultaneously intracellular pH (pHi) and extracellular pH (pHc) during long term storage of erythrocytes; pH was determined at both 4 degrees C and 37 degrees C. An equation describing the equilibrium distribution of membrane-permeant ions was derived by consideration of the electrochemical and osmotic constraints in the RBC suspension. Phosphates 108-122 glucose-6-phosphate isomerase Homo sapiens 198-201 3817608-1 1986 A 31P NMR method based on pH dependent variation of the chemical-shift-difference between the resonances of orthophosphate and methylphosphonate was used to measure simultaneously intracellular pH (pHi) and extracellular pH (pHc) during long term storage of erythrocytes; pH was determined at both 4 degrees C and 37 degrees C. An equation describing the equilibrium distribution of membrane-permeant ions was derived by consideration of the electrochemical and osmotic constraints in the RBC suspension. methylphosphonic acid 127-144 glucose-6-phosphate isomerase Homo sapiens 198-201 4041540-2 1985 We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). 2,3-dicyanohydroquinone 184-211 glucose-6-phosphate isomerase Homo sapiens 62-64 2995378-7 1985 Monitoring pHi with an intracellularly trapped, pH-sensitive, fluorescent dye, 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein, we demonstrated that IL 2 rapidly (less than 90 s) initiates an increase in pHi in IL 2-sensitive human and murine T cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 79-125 glucose-6-phosphate isomerase Homo sapiens 11-14 2995378-7 1985 Monitoring pHi with an intracellularly trapped, pH-sensitive, fluorescent dye, 2",7"-bis(carboxyethyl)-5,6-carboxyfluorescein, we demonstrated that IL 2 rapidly (less than 90 s) initiates an increase in pHi in IL 2-sensitive human and murine T cells. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 79-125 glucose-6-phosphate isomerase Homo sapiens 203-206 2995378-9 1985 Using partitioning of a weak acid, 5,5-dimethyl-2,4-oxazolidinedione, we confirmed that the IL 2-dependent increase in pHi is sustained for several hours and returns to near base-line levels by 18 h. We also investigated the consequence of preventing Na+/H+ exchange on the proliferative response induced by IL 2. Dimethadione 35-68 glucose-6-phosphate isomerase Homo sapiens 119-122 2413773-7 1985 Substitution of CSF Cl with isethionate resulted in marked alkalinization of pHi when [Cl]csf was depleted to 12 mM. Isethionic Acid 28-39 glucose-6-phosphate isomerase Homo sapiens 77-80 2996649-6 1985 From urine we extracted dinor metabolites of PGI according to a selective method. S-(2-(N,N-diisopropylamino)ethyl)isothiourea 24-29 glucose-6-phosphate isomerase Homo sapiens 45-48 2995444-1 1985 The relationship of intracellular pH (pHi) to superoxide radical (O2-) generation was investigated in chemotactic factor-stimulated human neutrophils. Superoxides 46-64 glucose-6-phosphate isomerase Homo sapiens 38-41 2995444-1 1985 The relationship of intracellular pH (pHi) to superoxide radical (O2-) generation was investigated in chemotactic factor-stimulated human neutrophils. Superoxides 66-68 glucose-6-phosphate isomerase Homo sapiens 38-41 2995444-2 1985 Exposure of cells to 100 nM N-formylmethionyl-leucyl-phenylalanine (FMLP) caused activation of Na/H exchange which, in 140 mM Na medium (pH0 7.40), led to a rise in pHi from 7.22 to 7.80. N-Formylmethionine Leucyl-Phenylalanine 28-66 glucose-6-phosphate isomerase Homo sapiens 165-168 2995444-2 1985 Exposure of cells to 100 nM N-formylmethionyl-leucyl-phenylalanine (FMLP) caused activation of Na/H exchange which, in 140 mM Na medium (pH0 7.40), led to a rise in pHi from 7.22 to 7.80. na medium 126-135 glucose-6-phosphate isomerase Homo sapiens 165-168 2995444-3 1985 This pHi change was sensitive to amiloride (apparent Ki 78 microM), an inhibitor of Na/H countertransport. Amiloride 33-42 glucose-6-phosphate isomerase Homo sapiens 5-8 2995444-5 1985 In the presence of 1 mM amiloride, which nearly blocked the pHi transient elicited by FMLP, or in the absence of external Na, where intracellular acidification was observed in FMLP-stimulated cells, O2- release was still roughly 25-45% of normal. Amiloride 24-33 glucose-6-phosphate isomerase Homo sapiens 60-63 2995444-5 1985 In the presence of 1 mM amiloride, which nearly blocked the pHi transient elicited by FMLP, or in the absence of external Na, where intracellular acidification was observed in FMLP-stimulated cells, O2- release was still roughly 25-45% of normal. Superoxides 199-201 glucose-6-phosphate isomerase Homo sapiens 60-63 2995444-8 1985 In each instance, the amount of O2- release correlated directly with pHi and was enhanced by intracellular alkalinization. Superoxides 32-34 glucose-6-phosphate isomerase Homo sapiens 69-72 2995444-9 1985 In the absence of FMLP, a rise in pHi to 7.7-7.8 by exposure of cells to 30 mM NH4Cl, 10 microM monensin (a Na/H exchanging ionophore), or after a prepulse with 18% CO2 did not result in O2- generation. Ammonium Chloride 79-84 glucose-6-phosphate isomerase Homo sapiens 34-37 2995444-9 1985 In the absence of FMLP, a rise in pHi to 7.7-7.8 by exposure of cells to 30 mM NH4Cl, 10 microM monensin (a Na/H exchanging ionophore), or after a prepulse with 18% CO2 did not result in O2- generation. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 165-168 glucose-6-phosphate isomerase Homo sapiens 34-37 2995444-9 1985 In the absence of FMLP, a rise in pHi to 7.7-7.8 by exposure of cells to 30 mM NH4Cl, 10 microM monensin (a Na/H exchanging ionophore), or after a prepulse with 18% CO2 did not result in O2- generation. Superoxides 166-168 glucose-6-phosphate isomerase Homo sapiens 34-37 2995444-13 1985 Together, these results indicate a modulating effect of pHi on O2- production and suggest that other functional responses of neutrophils may be regulated by their pHi. Superoxides 63-65 glucose-6-phosphate isomerase Homo sapiens 56-59 3009823-6 1986 When pHo was kept at 8.0, the amiloride-sensitive Na+ entry was abolished as pHi was increased from 6.4 to 7.8. Amiloride 30-39 glucose-6-phosphate isomerase Homo sapiens 77-80 3009823-8 1986 Furthermore, in the absence of a chemical gradient for Na+ (Nai+ = Nao+ = 15 mM, Em = +6.7 mV), an outward H+ gradient (pHi = 6.4, pHo = 8.0) promoted a net amiloride-sensitive Na+ uptake which was abolished at an external pH of 6.0. Amiloride 157-166 glucose-6-phosphate isomerase Homo sapiens 120-123 3810060-4 1986 Intracellular bicarbonate (HCO3i) and pH (pHi) were obtained by means of muscle total carbon dioxide method: a significant (p less than 0.001) reduction in both intracellular acid--base indexes was found in all patients (pHi 6.82 +/- 13 vs. 7.04 +/- 0.05; HCO3i 6.28 +/- 2.07 vs. 11.86 +/- 0.87). Carbon Dioxide 86-100 glucose-6-phosphate isomerase Homo sapiens 38-40 3810060-4 1986 Intracellular bicarbonate (HCO3i) and pH (pHi) were obtained by means of muscle total carbon dioxide method: a significant (p less than 0.001) reduction in both intracellular acid--base indexes was found in all patients (pHi 6.82 +/- 13 vs. 7.04 +/- 0.05; HCO3i 6.28 +/- 2.07 vs. 11.86 +/- 0.87). Carbon Dioxide 86-100 glucose-6-phosphate isomerase Homo sapiens 42-45 2997160-5 1985 Exposure of cells to 0.1 microM N-formyl-methionyl-leucyl-phenylalanine (FMLP) in 140 mM Na+ medium at extracellular pH (pHo) 7.40 led to a rise in pHi along an exponential time course (rate coefficient approximately 0.55 min-1). na+ medium 89-99 glucose-6-phosphate isomerase Homo sapiens 148-151 4037093-4 1985 Replacement of luminal Na+ with choline quickly (within 1 min) decreased pHi from 7.25 +/- 0.05 to 7.10 +/- 0.05 (P less than 0.001). Choline 32-39 glucose-6-phosphate isomerase Homo sapiens 73-76 4037093-6 1985 A reduction of luminal Na+ from 20 mM to 0 also reduced pHi from 7.15 +/- 0.06 to 7.04 +/- 0.08, and this pHi reduction was blocked by luminal addition of 1 mM amiloride. Amiloride 160-169 glucose-6-phosphate isomerase Homo sapiens 56-59 4037093-6 1985 A reduction of luminal Na+ from 20 mM to 0 also reduced pHi from 7.15 +/- 0.06 to 7.04 +/- 0.08, and this pHi reduction was blocked by luminal addition of 1 mM amiloride. Amiloride 160-169 glucose-6-phosphate isomerase Homo sapiens 106-109 4037093-8 1985 Replacement of bath Na+ with choline also reversibly reduced pHi from 7.27 +/- 0.02 to 7.15 +/- 0.02 (P less than 0.001), but this change was totally blocked by the presence of 1 mM SITS. Choline 29-36 glucose-6-phosphate isomerase Homo sapiens 61-64 4037093-8 1985 Replacement of bath Na+ with choline also reversibly reduced pHi from 7.27 +/- 0.02 to 7.15 +/- 0.02 (P less than 0.001), but this change was totally blocked by the presence of 1 mM SITS. 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid 182-186 glucose-6-phosphate isomerase Homo sapiens 61-64 3875620-3 1985 The pHi image was calculated from the data obtained after injection of [11C]5,5-dimethyl-2,4-oxazolidinedione and from the FVECW image. [11c]5,5-dimethyl-2,4-oxazolidinedione 71-109 glucose-6-phosphate isomerase Homo sapiens 4-7 3875620-8 1985 The increase in pHi was not correlated with changes in FVECW, CBF, or CMRO2, but there was a significant correlation with the decrease in oxygen extraction fraction in the same region. Oxygen 138-144 glucose-6-phosphate isomerase Homo sapiens 16-19 3875620-10 1985 An alkaline shift in pHi enhances the glycolysis rate and could explain why the glucose metabolism is less affected than the oxygen metabolism in recent cerebral infarction. Glucose 80-87 glucose-6-phosphate isomerase Homo sapiens 21-24 3875620-10 1985 An alkaline shift in pHi enhances the glycolysis rate and could explain why the glucose metabolism is less affected than the oxygen metabolism in recent cerebral infarction. Oxygen 125-131 glucose-6-phosphate isomerase Homo sapiens 21-24 3875620-11 1985 The pHi measured in the infarct could represent mainly the pHi of phagocytic cells that use aerobic glycolysis to synthesize hydrogen peroxide. Hydrogen Peroxide 125-142 glucose-6-phosphate isomerase Homo sapiens 4-7 3875620-11 1985 The pHi measured in the infarct could represent mainly the pHi of phagocytic cells that use aerobic glycolysis to synthesize hydrogen peroxide. Hydrogen Peroxide 125-142 glucose-6-phosphate isomerase Homo sapiens 59-62 4041540-2 1985 We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). 2,3-dicyanohydroquinone 184-211 glucose-6-phosphate isomerase Homo sapiens 66-69 4041540-2 1985 We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). 2,3-dicyanohydroquinone 184-211 glucose-6-phosphate isomerase Homo sapiens 66-68 4041540-2 1985 We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). 2,3-dicyanohydroquinone 213-221 glucose-6-phosphate isomerase Homo sapiens 62-64 4041540-2 1985 We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). 2,3-dicyanohydroquinone 213-221 glucose-6-phosphate isomerase Homo sapiens 66-69 4041540-2 1985 We have developed new methodology for measuring intracellular pH (pHi) in cultured cell monolayers and epithelia by analyzing the emission spectra of the trapped fluorescent pH probe, 1,4-dihydroxyphthalonitrile (1,4-DHPN). 2,3-dicyanohydroquinone 213-221 glucose-6-phosphate isomerase Homo sapiens 66-68 6532183-2 1984 The mitogenic activation sequence shows at least one cyclosporin sensitive step at or prior to the rise of cytoplasmic pH (pHi), which normally occurs after exposure to mitogen and which seems to be a permissive condition for initiation of DNA synthesis (S phase of the cell cycle). Cyclosporine 53-64 glucose-6-phosphate isomerase Homo sapiens 123-126 3892164-2 1985 The present study evaluates the relationship of plasma levels of thromboxane A2 and prostacyclin, measured by radioimmunoassay as the stable metabolites thromboxane B2 (TxB) and prostaglandin 6-K-F1 alpha (PGI) to the incidence of clinical ARDS. Thromboxane A2 65-79 glucose-6-phosphate isomerase Homo sapiens 206-209 3892164-2 1985 The present study evaluates the relationship of plasma levels of thromboxane A2 and prostacyclin, measured by radioimmunoassay as the stable metabolites thromboxane B2 (TxB) and prostaglandin 6-K-F1 alpha (PGI) to the incidence of clinical ARDS. Epoprostenol 84-96 glucose-6-phosphate isomerase Homo sapiens 206-209 3892164-2 1985 The present study evaluates the relationship of plasma levels of thromboxane A2 and prostacyclin, measured by radioimmunoassay as the stable metabolites thromboxane B2 (TxB) and prostaglandin 6-K-F1 alpha (PGI) to the incidence of clinical ARDS. prostaglandin 6-k-f1 alpha 178-204 glucose-6-phosphate isomerase Homo sapiens 206-209 3838109-1 1985 We measured urinary excretion of the principal metabolite of prostacyclin, PGI-M (2,3-dinor-6-keto-PGF1 alpha) in two patients with Shy-Drager syndrome and three with idiopathic orthostatic hypotension. 2,3-dinor-6-ketoprostaglandin F1alpha 82-109 glucose-6-phosphate isomerase Homo sapiens 75-78 6542618-1 1984 It is difficult to measure intracellular calcium concentrations in dividing embryos and, furthermore, these interact with pHi and with cyclic nucleotides. Calcium 41-48 glucose-6-phosphate isomerase Homo sapiens 122-125 4032303-3 1985 Leech Retzius neurones superfused with a pH 7.4 HCO3--free physiological saline were found to have a pHi of 7.3, too high to be explained by a passive distribution of H+ or OH-. Bicarbonates 48-52 glucose-6-phosphate isomerase Homo sapiens 101-104 4032303-3 1985 Leech Retzius neurones superfused with a pH 7.4 HCO3--free physiological saline were found to have a pHi of 7.3, too high to be explained by a passive distribution of H+ or OH-. Sodium Chloride 73-79 glucose-6-phosphate isomerase Homo sapiens 101-104 4032303-4 1985 To investigate pHi regulation the pHi was decreased by one of three methods: by exposure to propionate, by adding and then removing NH4Cl or by exposure to CO2. Propionates 92-102 glucose-6-phosphate isomerase Homo sapiens 34-37 4032303-4 1985 To investigate pHi regulation the pHi was decreased by one of three methods: by exposure to propionate, by adding and then removing NH4Cl or by exposure to CO2. Ammonium Chloride 132-137 glucose-6-phosphate isomerase Homo sapiens 34-37 4032303-4 1985 To investigate pHi regulation the pHi was decreased by one of three methods: by exposure to propionate, by adding and then removing NH4Cl or by exposure to CO2. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 156-159 glucose-6-phosphate isomerase Homo sapiens 34-37 4032303-6 1985 In HCO3--free solutions, pHi recovery from acidification was blocked by removing external Na or by amiloride (2 mM). Bicarbonates 3-7 glucose-6-phosphate isomerase Homo sapiens 25-28 4032303-6 1985 In HCO3--free solutions, pHi recovery from acidification was blocked by removing external Na or by amiloride (2 mM). Amiloride 99-108 glucose-6-phosphate isomerase Homo sapiens 25-28 4032303-8 1985 The anion exchange inhibitor SITS (4-acetamido-4"-isothiocyanato-stilbene-2,2"-disulphonic acid) also slowed pHi recovery in the presence of HCO3-. 4-acetamido-4"-isothiocyanato-stilbene-2,2"-disulphonic acid 35-95 glucose-6-phosphate isomerase Homo sapiens 109-112 4032303-10 1985 We conclude that in HCO3--free solutions pHi regulation is by a Na-H exchange system; but in the presence of HCO3- there is an additional mechanism which is probably a Na-dependent Cl-HCO3 exchanger. Bicarbonates 20-24 glucose-6-phosphate isomerase Homo sapiens 41-44 3996609-6 1985 In the presence of HCO3- and Cl- a volume increase will accompany the change in pHi. Bicarbonates 19-23 glucose-6-phosphate isomerase Homo sapiens 80-83 2985735-3 1985 During elevation of intracellular cAMP, intracellular Na+ activity (alpha Nai) and intracellular pH (pHi) decreased significantly. Cyclic AMP 34-38 glucose-6-phosphate isomerase Homo sapiens 101-104 2985735-6 1985 The rates of change of alpha Nai upon rapid alterations of mucosal [Na+] from 100 to 10 mM and from 10 to 100 mM were both decreased, and the rate of pHi recovery upon acid loading was also reduced by elevated cAMP levels. Cyclic AMP 210-214 glucose-6-phosphate isomerase Homo sapiens 150-153 2985735-9 1985 The results of measurements of pHi recovery at 10 and 100 mM mucosal [Na+] and a kinetic analysis of recovery as a function of pHi suggest that the main or sole mechanism of the inhibitory effect of cAMP is a reduction in the maximal rate of acid extrusion. Cyclic AMP 199-203 glucose-6-phosphate isomerase Homo sapiens 31-34 2985735-9 1985 The results of measurements of pHi recovery at 10 and 100 mM mucosal [Na+] and a kinetic analysis of recovery as a function of pHi suggest that the main or sole mechanism of the inhibitory effect of cAMP is a reduction in the maximal rate of acid extrusion. Cyclic AMP 199-203 glucose-6-phosphate isomerase Homo sapiens 127-130 2985735-13 1985 It is also possible that inhibition of Na+/H+ exchange by cAMP plays a role in the regulation of pHi in other cell types. Cyclic AMP 58-62 glucose-6-phosphate isomerase Homo sapiens 97-100 2985737-1 1985 The intracellular pH (pHi) of isolated human peripheral blood neutrophils was measured from the fluorescence of 6-carboxyfluorescein (6-CF) and from the equilibrium distribution of [14C]5,5-dimethyloxazolidine -2,4-dione (DMO). 6-carboxyfluorescein 112-132 glucose-6-phosphate isomerase Homo sapiens 22-25 2985737-1 1985 The intracellular pH (pHi) of isolated human peripheral blood neutrophils was measured from the fluorescence of 6-carboxyfluorescein (6-CF) and from the equilibrium distribution of [14C]5,5-dimethyloxazolidine -2,4-dione (DMO). 6-carboxyfluorescein 134-138 glucose-6-phosphate isomerase Homo sapiens 22-25 2985737-1 1985 The intracellular pH (pHi) of isolated human peripheral blood neutrophils was measured from the fluorescence of 6-carboxyfluorescein (6-CF) and from the equilibrium distribution of [14C]5,5-dimethyloxazolidine -2,4-dione (DMO). Carbon-14 182-185 glucose-6-phosphate isomerase Homo sapiens 22-25 2985737-1 1985 The intracellular pH (pHi) of isolated human peripheral blood neutrophils was measured from the fluorescence of 6-carboxyfluorescein (6-CF) and from the equilibrium distribution of [14C]5,5-dimethyloxazolidine -2,4-dione (DMO). Dimethadione 186-220 glucose-6-phosphate isomerase Homo sapiens 22-25 2985737-1 1985 The intracellular pH (pHi) of isolated human peripheral blood neutrophils was measured from the fluorescence of 6-carboxyfluorescein (6-CF) and from the equilibrium distribution of [14C]5,5-dimethyloxazolidine -2,4-dione (DMO). demethyloleuropein 222-225 glucose-6-phosphate isomerase Homo sapiens 22-25 2985737-2 1985 At an extracellular pH (pHo) of 7.40 in nominally CO2-free medium, the steady state pHi using either indicator was approximately 7.25. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 50-53 glucose-6-phosphate isomerase Homo sapiens 84-87 3978424-3 1985 In nominally HCO-3-free solutions, pHi recovery from acid loading was blocked by 10(-3)M amiloride. Bicarbonates 13-18 glucose-6-phosphate isomerase Homo sapiens 35-38 3978424-3 1985 In nominally HCO-3-free solutions, pHi recovery from acid loading was blocked by 10(-3)M amiloride. Amiloride 89-98 glucose-6-phosphate isomerase Homo sapiens 35-38 3978424-6 1985 The presence of a distinct, HCO-3-dependent pHi regulatory mechanism is postulated. 7 alpha-hydroxy-4-cholesten-3-one 28-31 glucose-6-phosphate isomerase Homo sapiens 44-47 6085015-5 1984 The results indicate that the transformed laryngeal cell line HEp-2 possesses a receptor-cAMP system preferentially activated by VIP (relative potencies: VIP greater than PHI much greater than other peptides of the secretin family), and suggest that this neuropeptide could modulate biological functions in normal laryngeal epithelia in man. Cyclic AMP 89-93 glucose-6-phosphate isomerase Homo sapiens 171-174 6332816-4 1984 When measured using [14C]-dimethyloxazolidinedione (DMO), pHi in the large cells of both species was approximately 0.15 units more alkaline than in the small cells. Carbon-14 21-24 glucose-6-phosphate isomerase Homo sapiens 58-61 6095096-5 1984 Using newly developed pH microelectrodes and fluorimetric techniques, we show that a tumour promoting phorbol ester and synthetic diacylglycerol, both potent activators of kinase C (refs 12-15), mimic the action of mitogens in rapidly elevating pHi in different cell types. Phorbol Esters 102-115 glucose-6-phosphate isomerase Homo sapiens 245-248 6095096-5 1984 Using newly developed pH microelectrodes and fluorimetric techniques, we show that a tumour promoting phorbol ester and synthetic diacylglycerol, both potent activators of kinase C (refs 12-15), mimic the action of mitogens in rapidly elevating pHi in different cell types. Diglycerides 130-144 glucose-6-phosphate isomerase Homo sapiens 245-248 6095096-7 1984 Finally, we suggest that an alkaline pHi shift, mediated by Na+/H+ exchange, may be a common signal in the action of those hormones which elicit the breakdown of inositol phospholipids. Phosphatidylinositols 162-184 glucose-6-phosphate isomerase Homo sapiens 37-40 6332816-4 1984 When measured using [14C]-dimethyloxazolidinedione (DMO), pHi in the large cells of both species was approximately 0.15 units more alkaline than in the small cells. Dimethadione 26-50 glucose-6-phosphate isomerase Homo sapiens 58-61 6332816-4 1984 When measured using [14C]-dimethyloxazolidinedione (DMO), pHi in the large cells of both species was approximately 0.15 units more alkaline than in the small cells. Dimethadione 52-55 glucose-6-phosphate isomerase Homo sapiens 58-61 6332816-5 1984 However, these differences were not detectable when pHi was measured with carboxylated fluorescein derivatives generated in situ by cytoplasmic enzymes. carboxylated fluorescein 74-98 glucose-6-phosphate isomerase Homo sapiens 52-55 6440212-7 1984 On the other hand, the much larger increase in PGH synthase than in PGI synthase in pregnant as compared to non-pregnant myometrium, may serve to promote preferential synthesis of prostaglandins that are potent myometrial stimulants and of critical importance in human parturition. Prostaglandins 180-194 glucose-6-phosphate isomerase Homo sapiens 68-71 6326603-9 1984 Moreover, additional evidence is presented in favor of a passive HCO3(-) efflux at steady-state pHi in the normal superfusate. Bicarbonates 65-69 glucose-6-phosphate isomerase Homo sapiens 96-99 6087813-1 1984 The cytoplasmic pH (pHi) of human blood neutrophils was measured using trapped carboxyfluorescein derivatives. 6-carboxyfluorescein 79-97 glucose-6-phosphate isomerase Homo sapiens 20-23 6330063-5 1984 When HF cells are acid-loaded by externally applied weak acids or by pretreatment with NH4+, pHi immediately recovers toward its resting value (approximately 7.05). Ammonium Compounds 87-91 glucose-6-phosphate isomerase Homo sapiens 93-96 6330063-7 1984 Recovery of pHi and concomitant Na+/H+ fluxes are reversibly inhibited by amiloride (half-maximal effect at approximately 0.1 mM). Amiloride 74-83 glucose-6-phosphate isomerase Homo sapiens 12-15 6330063-8 1984 The rate of pHi recovery from an acid load depends on external Na+ (half-maximal rate at approximately 35 mM), but is independent of external anions (HCO3-, Cl-) and is not affected by membrane depolarization. Bicarbonates 150-154 glucose-6-phosphate isomerase Homo sapiens 12-15 6330063-12 1984 The response of pHi to alkaline pHo shifts is abolished by amiloride and by Na+ removal. Amiloride 59-68 glucose-6-phosphate isomerase Homo sapiens 16-19 6330063-13 1984 It is concluded that pHi in HF cells is closely regulated by an amiloride-sensitive, reversible Na+/H+ exchanger, which is driven by the transmembrane concentration gradients for Na+ and H+. Amiloride 64-73 glucose-6-phosphate isomerase Homo sapiens 21-24 6736918-12 1984 The pHi changes were larger in 10 mM HCO3-Ringer"s than in 1 mM HEPES-Ringer"s, which suggests that HCO3- is transported in exchange for Cl-. Bicarbonates 37-41 glucose-6-phosphate isomerase Homo sapiens 4-7 6736918-12 1984 The pHi changes were larger in 10 mM HCO3-Ringer"s than in 1 mM HEPES-Ringer"s, which suggests that HCO3- is transported in exchange for Cl-. HEPES 64-69 glucose-6-phosphate isomerase Homo sapiens 4-7 6736918-12 1984 The pHi changes were larger in 10 mM HCO3-Ringer"s than in 1 mM HEPES-Ringer"s, which suggests that HCO3- is transported in exchange for Cl-. Bicarbonates 100-104 glucose-6-phosphate isomerase Homo sapiens 4-7 6736918-13 1984 In both HEPES- and HCO3-Ringer"s, SITS inhibited the pHi changes. HEPES 8-13 glucose-6-phosphate isomerase Homo sapiens 53-56 6736918-13 1984 In both HEPES- and HCO3-Ringer"s, SITS inhibited the pHi changes. Bicarbonates 19-23 glucose-6-phosphate isomerase Homo sapiens 53-56 6538853-5 1984 The schism in carbohydrate metabolism in the pgi- fibroblasts is clearly reflected through the development of the metabolically mediated curb of the hexose transport or uptake system. Carbohydrates 14-26 glucose-6-phosphate isomerase Homo sapiens 45-48 6538853-5 1984 The schism in carbohydrate metabolism in the pgi- fibroblasts is clearly reflected through the development of the metabolically mediated curb of the hexose transport or uptake system. Hexoses 149-155 glucose-6-phosphate isomerase Homo sapiens 45-48 6231945-1 1984 Platelet glycoprotein I (GPI) is known to be required for the interaction of platelets with ristocetin and factor VIII:von Willebrand factor (VIII:vWf). Ristocetin 92-102 glucose-6-phosphate isomerase Homo sapiens 9-23 6370554-9 1984 Endogenous prostacyclin biosynthesis was measured by excretion of the major urinary metabolite 2,3-dinor-6-keto-PGF1 alpha (PGI-M). Epoprostenol 11-23 glucose-6-phosphate isomerase Homo sapiens 124-127 6370554-9 1984 Endogenous prostacyclin biosynthesis was measured by excretion of the major urinary metabolite 2,3-dinor-6-keto-PGF1 alpha (PGI-M). 2,3-dinor-6-ketoprostaglandin F1alpha 95-122 glucose-6-phosphate isomerase Homo sapiens 124-127 6370554-10 1984 Whereas aspirin, 325 mg, reduced PGI-M excretion a mean 29%, excretion increased 48% and 100% after CGS 13080, 100 mg and 200 mg. Aspirin, 20 mg, did not alter prostacyclin biosynthesis. Aspirin 8-15 glucose-6-phosphate isomerase Homo sapiens 33-36 6326601-5 1984 Similar evidence suggests that pHi changes also influence intracellular adenosine 3",5"-cyclic monophosphate levels, and vice versa. Cyclic AMP 72-108 glucose-6-phosphate isomerase Homo sapiens 31-34 6231945-1 1984 Platelet glycoprotein I (GPI) is known to be required for the interaction of platelets with ristocetin and factor VIII:von Willebrand factor (VIII:vWf). Ristocetin 92-102 glucose-6-phosphate isomerase Homo sapiens 25-28 6231945-6 1984 Platelets from two patients with Bernard-Soulier syndrome, congenitally deficient in GPI, both aggregated and released 14C-serotonin normally when exposed to latex particles coated with IgG. 14c-serotonin 119-132 glucose-6-phosphate isomerase Homo sapiens 85-88 6231945-6 1984 Platelets from two patients with Bernard-Soulier syndrome, congenitally deficient in GPI, both aggregated and released 14C-serotonin normally when exposed to latex particles coated with IgG. Latex 158-163 glucose-6-phosphate isomerase Homo sapiens 85-88 6419623-6 1983 With exposure to increasing extracellular PCO2, there is a polynomial decrease in pHi. pco2 42-46 glucose-6-phosphate isomerase Homo sapiens 82-85 6548019-1 1984 The detection of the C-terminal amide structure in porcine intestinal extracts has led to the discovery of a 27 amino acid residue peptide designated PHI (PHI-27, peptide HI). Amides 32-37 glucose-6-phosphate isomerase Homo sapiens 150-153 6548019-1 1984 The detection of the C-terminal amide structure in porcine intestinal extracts has led to the discovery of a 27 amino acid residue peptide designated PHI (PHI-27, peptide HI). Amides 32-37 glucose-6-phosphate isomerase Homo sapiens 155-161 6322760-2 1984 We show that vanadate, previously found to act as a mitogen for a number of cells, reversibly activates Na+/H+ exchange at micromolar concentrations in A431 cells, leading to a large increase of pHi. Vanadates 13-21 glucose-6-phosphate isomerase Homo sapiens 195-198 6322760-4 1984 Elevation of pHi by vanadate and by epidermal growth factor (EGF) both display similar kinetics, and both EGF and vanadate stimulate the rate of pHi recovery following an acute acid load, suggesting that vanadate stimulates Na+/H+ exchange by a mechanism similar to that of polypeptide growth factor stimulation. Vanadates 20-28 glucose-6-phosphate isomerase Homo sapiens 13-16 6322760-4 1984 Elevation of pHi by vanadate and by epidermal growth factor (EGF) both display similar kinetics, and both EGF and vanadate stimulate the rate of pHi recovery following an acute acid load, suggesting that vanadate stimulates Na+/H+ exchange by a mechanism similar to that of polypeptide growth factor stimulation. Vanadates 114-122 glucose-6-phosphate isomerase Homo sapiens 145-148 6322760-4 1984 Elevation of pHi by vanadate and by epidermal growth factor (EGF) both display similar kinetics, and both EGF and vanadate stimulate the rate of pHi recovery following an acute acid load, suggesting that vanadate stimulates Na+/H+ exchange by a mechanism similar to that of polypeptide growth factor stimulation. Vanadates 114-122 glucose-6-phosphate isomerase Homo sapiens 145-148 6746191-5 1984 Significant decreases in systolic blood pressure, heart rate, plasma noradrenaline and urinary excretion of noradrenaline were observed in Gp I but not in Gp II. Norepinephrine 69-82 glucose-6-phosphate isomerase Homo sapiens 139-143 6746191-5 1984 Significant decreases in systolic blood pressure, heart rate, plasma noradrenaline and urinary excretion of noradrenaline were observed in Gp I but not in Gp II. Norepinephrine 108-121 glucose-6-phosphate isomerase Homo sapiens 139-143 6419623-7 1983 The pHi tended to be more alkaline in SO2-4 -Ringer, suggesting the presence of a HCO-3/Cl- exchange mechanism. 7 alpha-hydroxy-4-cholesten-3-one 82-85 glucose-6-phosphate isomerase Homo sapiens 4-7 6195155-5 1983 We also described the presence of transmembrane, amiloride-sensitive Na+/H+ exchange in A431 cells using a new fluorescence technique for the measurement of intracellular pH (pHi) based on the incorporation of fluorescein-dextran into the cell cytoplasm. fluorescein-dextran 210-229 glucose-6-phosphate isomerase Homo sapiens 171-173 6355181-7 1983 Endogenous prostacyclin biosynthesis was assessed by measurement of the major urinary metabolite of prostacyclin, 2,3-dinor-6-keto-PGF1 alpha (PGI-M). Epoprostenol 11-23 glucose-6-phosphate isomerase Homo sapiens 143-146 6355181-8 1983 PGI-M excretion was increased by dazoxiben; it rose a mean 2.4-fold from predosing control values at 0-6 h after administration of the highest dose studied (200 mg). dazoxiben 33-42 glucose-6-phosphate isomerase Homo sapiens 0-3 6420546-5 1983 When pHi was lowered to about 6.8 by replacing the HEPES by 5% CO2, 24 mM-HCO3 (constant pHo), it recovered at a very slow rate of 0.025 +/- 0.011 delta pHi h-1 (n = 6). HEPES 51-56 glucose-6-phosphate isomerase Homo sapiens 5-8 6420546-5 1983 When pHi was lowered to about 6.8 by replacing the HEPES by 5% CO2, 24 mM-HCO3 (constant pHo), it recovered at a very slow rate of 0.025 +/- 0.011 delta pHi h-1 (n = 6). N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 63-66 glucose-6-phosphate isomerase Homo sapiens 5-8 6420546-5 1983 When pHi was lowered to about 6.8 by replacing the HEPES by 5% CO2, 24 mM-HCO3 (constant pHo), it recovered at a very slow rate of 0.025 +/- 0.011 delta pHi h-1 (n = 6). Bicarbonates 74-78 glucose-6-phosphate isomerase Homo sapiens 5-8 6420546-6 1983 When all the Na was replaced by N-methyl-D-glucamine (initial pHi 7.20 +/- 0.04, initial Vm -89 +/- 1.5 mV, n = 8), this slow alkalinization was converted into a slow acidification at a rate of 0.069 +/- 0.024 delta pHi h-1. Meglumine 32-52 glucose-6-phosphate isomerase Homo sapiens 62-65 6420546-6 1983 When all the Na was replaced by N-methyl-D-glucamine (initial pHi 7.20 +/- 0.04, initial Vm -89 +/- 1.5 mV, n = 8), this slow alkalinization was converted into a slow acidification at a rate of 0.069 +/- 0.024 delta pHi h-1. Meglumine 32-52 glucose-6-phosphate isomerase Homo sapiens 216-219 6420546-12 1983 When pHi of depolarized fibres (50 mM-K) was lowered to about 6.8 by the weak acid dimethyl-2,4-oxazolidinedione (DMO), it recovered at a rate of 0.12 delta pHi h-1 in two experiments. dimethyl-2,4-oxazolidinedione 83-112 glucose-6-phosphate isomerase Homo sapiens 5-8 6420546-12 1983 When pHi of depolarized fibres (50 mM-K) was lowered to about 6.8 by the weak acid dimethyl-2,4-oxazolidinedione (DMO), it recovered at a rate of 0.12 delta pHi h-1 in two experiments. demethyloleuropein 114-117 glucose-6-phosphate isomerase Homo sapiens 5-8 6420546-12 1983 When pHi of depolarized fibres (50 mM-K) was lowered to about 6.8 by the weak acid dimethyl-2,4-oxazolidinedione (DMO), it recovered at a rate of 0.12 delta pHi h-1 in two experiments. demethyloleuropein 114-117 glucose-6-phosphate isomerase Homo sapiens 157-160 6420546-13 1983 In fibres depolarized in 50 mM-K and constant Cl, either 0.1 mM-SITS or 0.5 mM-amiloride slowed pHi recovery from CO2 exposure by about 50%. Amiloride 79-88 glucose-6-phosphate isomerase Homo sapiens 96-99 6195155-5 1983 We also described the presence of transmembrane, amiloride-sensitive Na+/H+ exchange in A431 cells using a new fluorescence technique for the measurement of intracellular pH (pHi) based on the incorporation of fluorescein-dextran into the cell cytoplasm. fluorescein-dextran 210-229 glucose-6-phosphate isomerase Homo sapiens 175-178 6195155-7 1983 In this paper, 4",5"-dimethylfluorescein (pKa 6.75) was coupled to dextran, allowing increased pH sensitivity of the fluorescence assay in the physiological range. 4',5'-dimethylfluorescein 15-40 glucose-6-phosphate isomerase Homo sapiens 95-97 6195155-7 1983 In this paper, 4",5"-dimethylfluorescein (pKa 6.75) was coupled to dextran, allowing increased pH sensitivity of the fluorescence assay in the physiological range. Dextrans 67-74 glucose-6-phosphate isomerase Homo sapiens 95-97 6195155-8 1983 Using this improved assay, basic features of pHi regulation in A431 cells are documented, including the role of Na+/H+ exchange and Na+-linked C1-/HCO3-exchange in acid extrusion. Bicarbonates 147-151 glucose-6-phosphate isomerase Homo sapiens 45-48 6195155-10 1983 The pHi increase is half-maximal at 5-10 ng/ml of EGF, is dependent on external Na+, independent of external Ca2+, and inhibited by millimolar amiloride. Amiloride 143-152 glucose-6-phosphate isomerase Homo sapiens 4-7 6305208-4 1983 1) Oxytocin increased the pHi when either serosal bicarbonate or Tris buffers was used and even in the presence of a low mucosal pH (Tris buffer, pH 5.8). Bicarbonates 50-61 glucose-6-phosphate isomerase Homo sapiens 26-29 6418691-5 1983 The nitrogen absorption and digestibility of the amino acid mixtures offered to Gp I and Gp II was significantly higher than that of the controls. Nitrogen 4-12 glucose-6-phosphate isomerase Homo sapiens 80-94 6305208-4 1983 1) Oxytocin increased the pHi when either serosal bicarbonate or Tris buffers was used and even in the presence of a low mucosal pH (Tris buffer, pH 5.8). Tromethamine 65-69 glucose-6-phosphate isomerase Homo sapiens 26-29 6876366-13 1983 The activity of phosphoglucose isomerase was slightly inhibited by 10(-3) M paromomycin sulfate while those of hexokinase, phosphofructokinase and glucose-6-phosphate dehydrogenase were not inhibited. Paromomycin 76-95 glucose-6-phosphate isomerase Homo sapiens 16-40 6842177-10 1983 This net efflux required external HCO-3, external Na+, an acid pHi, internal ATP, and was blocked by SITS. Bicarbonates 34-39 glucose-6-phosphate isomerase Homo sapiens 63-66 6842177-11 1983 We conclude that the pHi-regulating system mediates the obligate net influx of HCO-3 (or equivalent species) and Na+ and the net efflux of Cl- in the stoichiometry of 2:1:1. Bicarbonates 79-84 glucose-6-phosphate isomerase Homo sapiens 21-24 6897744-0 1982 Vasoactive intestinal peptide (VIP) and peptide having N-terminal histidine and C-terminal isoleucine amide (PHI) stimulate adenylate cyclase activity in human heart membranes. L-isoleucinamide 91-107 glucose-6-phosphate isomerase Homo sapiens 109-112 6295181-7 1983 Alteration of pHi, and not pHo, induces similar effects on glucose-induced electrical and secretory events. Glucose 59-66 glucose-6-phosphate isomerase Homo sapiens 14-17 6229300-4 1983 GPI consists of two glycopeptides, an alpha and a beta chain connected by a disulphide bridge. Glycopeptides 20-33 glucose-6-phosphate isomerase Homo sapiens 0-3 6229300-4 1983 GPI consists of two glycopeptides, an alpha and a beta chain connected by a disulphide bridge. disulphide 76-86 glucose-6-phosphate isomerase Homo sapiens 0-3 7159659-7 1982 The GPI enzyme allows for the interconversion of glucose-6-PO4 and fructose-6-PO4. glucose-6-po4 49-62 glucose-6-phosphate isomerase Homo sapiens 4-7 7159659-7 1982 The GPI enzyme allows for the interconversion of glucose-6-PO4 and fructose-6-PO4. fructose-6-po4 67-81 glucose-6-phosphate isomerase Homo sapiens 4-7 7159659-10 1982 Results from Experiment 3 indicated a marked increase (P less than 0.01) in GPI activity in uterine flushings from gilts treated with estradiol valerate. Estradiol 134-152 glucose-6-phosphate isomerase Homo sapiens 76-79 7119733-9 1982 After inhibition of Na+-H+ exchange by mucosal perfusion with amiloride (1 mM) or by complete Na+ replacement with TMA+, phi fell reversibly by 0.15 and 0.22 pH units, respectively. Amiloride 62-71 glucose-6-phosphate isomerase Homo sapiens 121-124 7138551-0 1982 Schism and complementation of hexose-mediated transport regulation as illustrated in a fibroblast mutant lacking phosphoglucose-isomerase. Hexoses 30-36 glucose-6-phosphate isomerase Homo sapiens 113-137 7119733-9 1982 After inhibition of Na+-H+ exchange by mucosal perfusion with amiloride (1 mM) or by complete Na+ replacement with TMA+, phi fell reversibly by 0.15 and 0.22 pH units, respectively. 4,4-dimethylcholesta-8,14-dien-3-ol 115-119 glucose-6-phosphate isomerase Homo sapiens 121-124 7304732-2 1981 Both propionate and DMO caused an initial drop in pHi, followed by a partial recovery to the final steady pHi of 7.16. Propionates 5-15 glucose-6-phosphate isomerase Homo sapiens 50-53 7036751-4 1982 The relationship between the change in pHi and percent change in glycolytic flux was the same regardless of whether the effects were produced by insulin or by changing CO2. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 168-171 glucose-6-phosphate isomerase Homo sapiens 39-42 7319892-3 1981 Intracellular pH (pHi) was calculated both in Cl--free and Cl--containing hearts from the distribution of 14C-labeled 5",5"-dimethyloxazolidine-2,4-dione. Carbon-14 106-109 glucose-6-phosphate isomerase Homo sapiens 18-21 7319892-3 1981 Intracellular pH (pHi) was calculated both in Cl--free and Cl--containing hearts from the distribution of 14C-labeled 5",5"-dimethyloxazolidine-2,4-dione. Dimethadione 118-153 glucose-6-phosphate isomerase Homo sapiens 18-21 7319892-6 1981 Increasing PCO2 at constant perfusate HCO-3 resulted in changes in pHi and cell HCO-3 (HCO-3i) that were similar in both Cl--free and Cl--containing hearts. pco2 11-15 glucose-6-phosphate isomerase Homo sapiens 67-70 6947244-3 1981 Porcine PHI was found in the intestinal extract by the presence of its COOH-terminal isoleucine amide structure. L-isoleucinamide 85-101 glucose-6-phosphate isomerase Homo sapiens 8-11 7304732-2 1981 Both propionate and DMO caused an initial drop in pHi, followed by a partial recovery to the final steady pHi of 7.16. Propionates 5-15 glucose-6-phosphate isomerase Homo sapiens 106-109 7304732-2 1981 Both propionate and DMO caused an initial drop in pHi, followed by a partial recovery to the final steady pHi of 7.16. Dimethadione 20-23 glucose-6-phosphate isomerase Homo sapiens 50-53 7304732-2 1981 Both propionate and DMO caused an initial drop in pHi, followed by a partial recovery to the final steady pHi of 7.16. Dimethadione 20-23 glucose-6-phosphate isomerase Homo sapiens 106-109 7304732-3 1981 In the presence of 6 mM HCO3(-) (pH0 7.8), the final pHi was 7.31 for either weak acid. Bicarbonates 24-28 glucose-6-phosphate isomerase Homo sapiens 53-56 7304732-4 1981 In other experiments, pHi was initially lowered by temporarily exposing the fiber to NH4Cl-containing solution. Ammonium Chloride 85-90 glucose-6-phosphate isomerase Homo sapiens 22-25 7304732-11 1981 Since exposure of the cell to either of the weak acids lowered pHi and since the rate of anion exchange fluxes is known to increase when pHi is lowered, propionate most likely stimulated anion fluxes indirectly by lowering pHi. Propionates 153-163 glucose-6-phosphate isomerase Homo sapiens 137-140 7304732-11 1981 Since exposure of the cell to either of the weak acids lowered pHi and since the rate of anion exchange fluxes is known to increase when pHi is lowered, propionate most likely stimulated anion fluxes indirectly by lowering pHi. Propionates 153-163 glucose-6-phosphate isomerase Homo sapiens 137-140 7024780-1 1981 Several metabolic compounds have been found to be competitive inhibitors of the anomerase activity of phosphoglucose isomerase (EC 5.3.1.9).Ki values for erythrose 4-phosphate, 6-phosphogluconate, and fructose 1,6-bisphosphate for the anomerase reaction are 0.32 muM, 21 muM, and 84 muM respectively at 0 degree and pH 8.2. erythrose 4-phosphate 154-175 glucose-6-phosphate isomerase Homo sapiens 102-126 7024780-1 1981 Several metabolic compounds have been found to be competitive inhibitors of the anomerase activity of phosphoglucose isomerase (EC 5.3.1.9).Ki values for erythrose 4-phosphate, 6-phosphogluconate, and fructose 1,6-bisphosphate for the anomerase reaction are 0.32 muM, 21 muM, and 84 muM respectively at 0 degree and pH 8.2. 6-phosphogluconic acid 177-195 glucose-6-phosphate isomerase Homo sapiens 102-126 7024780-1 1981 Several metabolic compounds have been found to be competitive inhibitors of the anomerase activity of phosphoglucose isomerase (EC 5.3.1.9).Ki values for erythrose 4-phosphate, 6-phosphogluconate, and fructose 1,6-bisphosphate for the anomerase reaction are 0.32 muM, 21 muM, and 84 muM respectively at 0 degree and pH 8.2. Fructose 201-209 glucose-6-phosphate isomerase Homo sapiens 102-126 7024780-1 1981 Several metabolic compounds have been found to be competitive inhibitors of the anomerase activity of phosphoglucose isomerase (EC 5.3.1.9).Ki values for erythrose 4-phosphate, 6-phosphogluconate, and fructose 1,6-bisphosphate for the anomerase reaction are 0.32 muM, 21 muM, and 84 muM respectively at 0 degree and pH 8.2. 1,6-bisphosphate 210-226 glucose-6-phosphate isomerase Homo sapiens 102-126 7320867-7 1981 When the cytoplasm was acidified (by NH4Cl loading, CO2 application, or HCl injection), pHi recovered towards its resting value. Ammonium Chloride 37-42 glucose-6-phosphate isomerase Homo sapiens 88-91 6790498-2 1981 The intracellular pH (pHi) was determined from the distribution of 14C-labeled 5,5-dimethyloxazoladine-2,4-dione, alpha- and beta-receptor antagonists were used to block the effects of endogenous catecholamines. Carbon-14 67-70 glucose-6-phosphate isomerase Homo sapiens 22-25 6790498-2 1981 The intracellular pH (pHi) was determined from the distribution of 14C-labeled 5,5-dimethyloxazoladine-2,4-dione, alpha- and beta-receptor antagonists were used to block the effects of endogenous catecholamines. 5,5-dimethyloxazoladine-2,4-dione 79-112 glucose-6-phosphate isomerase Homo sapiens 22-25 7320867-7 1981 When the cytoplasm was acidified (by NH4Cl loading, CO2 application, or HCl injection), pHi recovered towards its resting value. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 52-55 glucose-6-phosphate isomerase Homo sapiens 88-91 7320867-7 1981 When the cytoplasm was acidified (by NH4Cl loading, CO2 application, or HCl injection), pHi recovered towards its resting value. Hydrochloric Acid 72-75 glucose-6-phosphate isomerase Homo sapiens 88-91 7019429-3 1981 Preincubation of washed ram spermatozoa with 0.025% formaldehyde increased GPI levels but decreased LDH concentration in the extracellular fluid while hexokinase release was unaffected. Formaldehyde 52-64 glucose-6-phosphate isomerase Homo sapiens 75-78 7320867-15 1981 Reducing the external HCO3(-) concentration from 5 mM to 0 mM slowed pHi recovery by an average of about 45%. Bicarbonates 22-29 glucose-6-phosphate isomerase Homo sapiens 69-72 7320867-21 1981 In the presence of the anion exchange inhibitor SITS (4-acetamide-4"-isothiocyanostilbene-2,2"-disulphonic acid), pHi recovery was slowed to the rate which was normally seen in HCO3(-)-free Ringer solution. 4-acetamide-4"-isothiocyanostilbene-2,2"-disulphonic acid 54-111 glucose-6-phosphate isomerase Homo sapiens 114-117 7320867-21 1981 In the presence of the anion exchange inhibitor SITS (4-acetamide-4"-isothiocyanostilbene-2,2"-disulphonic acid), pHi recovery was slowed to the rate which was normally seen in HCO3(-)-free Ringer solution. Bicarbonates 177-182 glucose-6-phosphate isomerase Homo sapiens 114-117 7320867-22 1981 SITS abolished the dependence of pHi recovery on the external HCO3(-) concentration. Bicarbonates 62-66 glucose-6-phosphate isomerase Homo sapiens 33-36 7320867-24 1981 It is concluded that pHi regulation in crayfish neurones involves two separate mechanisms: a Na+-dependent, HCO3(-)-independent acid extrusion process, and a Cl---HCO3(-) exchange which is probably also Na+-dependent. Bicarbonates 108-112 glucose-6-phosphate isomerase Homo sapiens 21-24 7320867-24 1981 It is concluded that pHi regulation in crayfish neurones involves two separate mechanisms: a Na+-dependent, HCO3(-)-independent acid extrusion process, and a Cl---HCO3(-) exchange which is probably also Na+-dependent. Bicarbonates 163-167 glucose-6-phosphate isomerase Homo sapiens 21-24 7013841-2 1981 Reversing the Na+ free energy gradient by substituting either Mg2+ or choline for extracellular Na+ converts the effect of insulin to a decrease in pHi, indicating that the action of insulin upon pHi is determined by the Na+ free energy gradient. magnesium ion 62-66 glucose-6-phosphate isomerase Homo sapiens 148-151 7225356-0 1981 Synthesis of 1-chloro-2-oxohexanol 6-phosphate, a covalent active-site reagent for phosphoglucose isomerase. 1-chloro-2-ketohexanol-6-phosphate 13-46 glucose-6-phosphate isomerase Homo sapiens 83-107 7013757-3 1981 The technique involves three steps: (i) immobilization of growing cell colonies in agar gel containing antibody that specifically reacts with human-type PGI; (ii) lysis of the embedded cells with Triton X-100 to release enzyme antigens and precipitate as an immune complex; and (iii) visualization of the antibody-fixed enzymes by histochemical activity staining. Agar 83-87 glucose-6-phosphate isomerase Homo sapiens 153-156 7013841-2 1981 Reversing the Na+ free energy gradient by substituting either Mg2+ or choline for extracellular Na+ converts the effect of insulin to a decrease in pHi, indicating that the action of insulin upon pHi is determined by the Na+ free energy gradient. Choline 70-77 glucose-6-phosphate isomerase Homo sapiens 148-151 7013841-5 1981 This drug also blocks the decrease in pHi by insulin when Mg2+ is substituted for Na+ in the Ringer. magnesium ion 58-62 glucose-6-phosphate isomerase Homo sapiens 38-41 7013841-6 1981 In Ringer containing Na+, the increase in pHi by insulin occurs when both metabolic and atmospheric sources of CO2 are eliminated by using a 100% N2 atmosphere. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 111-114 glucose-6-phosphate isomerase Homo sapiens 42-45 7013841-6 1981 In Ringer containing Na+, the increase in pHi by insulin occurs when both metabolic and atmospheric sources of CO2 are eliminated by using a 100% N2 atmosphere. Nitrogen 146-148 glucose-6-phosphate isomerase Homo sapiens 42-45 6262384-3 1981 In addition, Phi bodies were demonstrated in AML blasts only when DAB was used; Phi bodies were also observed in two out of seven cases of chronic granulocytic leukaemia in "myeloid" blast crisis but were not seen in any case of acute lymphoblastic leukaemia. 3,3'-Diaminobenzidine 66-69 glucose-6-phosphate isomerase Homo sapiens 13-16 6262384-4 1981 Phi bodies were more numerous when the reaction was carried out at pH 9.7, and their number was significantly reduced in the presence of 3-amino 1,2,4-triazole. Amitrole 137-159 glucose-6-phosphate isomerase Homo sapiens 0-3 475753-3 1979 For glucose 6-phosphate dehydrogenase, hexokinase and fumarase, enzyme activity became detectable (about 0.05% of the fully hydrated rate) when the water content was about 0.2g/g of reaction mixture, and for phosphoglucose isomerase, around 0.15g/g of reaction mixture. Water 148-153 glucose-6-phosphate isomerase Homo sapiens 208-232 6257118-1 1981 The time course of the intracellular pH (pHi) of barnacle muscle fibers was followed using microelectrodes while the fibers were exposed to 15 mM of the weak acid 5,5-dimethyloxazolidine-2,4-dione (DMO) at pH 6.5. Dimethadione 163-196 glucose-6-phosphate isomerase Homo sapiens 41-44 6257118-1 1981 The time course of the intracellular pH (pHi) of barnacle muscle fibers was followed using microelectrodes while the fibers were exposed to 15 mM of the weak acid 5,5-dimethyloxazolidine-2,4-dione (DMO) at pH 6.5. Dimethadione 198-201 glucose-6-phosphate isomerase Homo sapiens 41-44 7411455-12 1980 The spikes disappeared when pHi was increased again following the addition of 10 mM-bicarbonate to the external solution. Bicarbonates 84-95 glucose-6-phosphate isomerase Homo sapiens 28-31 7411455-13 1980 CO2-saturated saline, which decreased pHi to 6 . co2-saturated saline 0-20 glucose-6-phosphate isomerase Homo sapiens 38-41 7411455-17 1980 External tetraethylammonium (TEA), at concentrations which reproduced the pHi = 6 . Tetraethylammonium 9-27 glucose-6-phosphate isomerase Homo sapiens 74-77 7411455-17 1980 External tetraethylammonium (TEA), at concentrations which reproduced the pHi = 6 . Tetraethylammonium 29-32 glucose-6-phosphate isomerase Homo sapiens 74-77 6990147-0 1980 Interaction of a newly isolated intestinal polypeptide (PHI) with glucose and arginine to effect the secretion of insulin and glucagon. Glucose 66-73 glucose-6-phosphate isomerase Homo sapiens 56-59 6990147-0 1980 Interaction of a newly isolated intestinal polypeptide (PHI) with glucose and arginine to effect the secretion of insulin and glucagon. Arginine 78-86 glucose-6-phosphate isomerase Homo sapiens 56-59 6990147-0 1980 Interaction of a newly isolated intestinal polypeptide (PHI) with glucose and arginine to effect the secretion of insulin and glucagon. Glucagon 126-134 glucose-6-phosphate isomerase Homo sapiens 56-59 43394-0 1979 Recovery of pHi in snail neurones exposed to high external potassium [proceedings]. Potassium 59-68 glucose-6-phosphate isomerase Homo sapiens 12-15 7463064-1 1981 Incubation of synaptosomes together with 1-acyl-2-[14C]arachidonoyl-sn-glycerophosphoinositols (GPI) and sodium deoxycholate yielded diacylglycerols and free arachidonic acid. 1-acyl-2-[14c]arachidonoyl-sn-glycerophosphoinositols 41-94 glucose-6-phosphate isomerase Homo sapiens 96-99 7463064-1 1981 Incubation of synaptosomes together with 1-acyl-2-[14C]arachidonoyl-sn-glycerophosphoinositols (GPI) and sodium deoxycholate yielded diacylglycerols and free arachidonic acid. Diglycerides 133-148 glucose-6-phosphate isomerase Homo sapiens 96-99 7463064-1 1981 Incubation of synaptosomes together with 1-acyl-2-[14C]arachidonoyl-sn-glycerophosphoinositols (GPI) and sodium deoxycholate yielded diacylglycerols and free arachidonic acid. Arachidonic Acid 158-174 glucose-6-phosphate isomerase Homo sapiens 96-99 7463064-2 1981 Diacylglycerol formation is attributed to hydrolysis by the diacyl-GPI-specific phospholipase C (EC 3.1.4.10), and this reaction requires sodium deoxycholate for optimal activity. Diglycerides 0-14 glucose-6-phosphate isomerase Homo sapiens 67-70 7463064-2 1981 Diacylglycerol formation is attributed to hydrolysis by the diacyl-GPI-specific phospholipase C (EC 3.1.4.10), and this reaction requires sodium deoxycholate for optimal activity. Deoxycholic Acid 138-157 glucose-6-phosphate isomerase Homo sapiens 67-70 7463064-3 1981 The free arachidonic acid formed is attributed to hydrolysis of diacyl-GPI by phospholipase A (EC 3.1.1.5). Arachidonic Acid 9-25 glucose-6-phosphate isomerase Homo sapiens 71-74 6460465-3 1981 The procedure is based on the conversion of glucose-6-phosphate to 1,3-diphosphoglycerate (1,3-DPG) which is catalyzed by the sequential action of the GPI, PFK, AL and GAPD. Glucose-6-Phosphate 44-63 glucose-6-phosphate isomerase Homo sapiens 151-154 6460465-3 1981 The procedure is based on the conversion of glucose-6-phosphate to 1,3-diphosphoglycerate (1,3-DPG) which is catalyzed by the sequential action of the GPI, PFK, AL and GAPD. glycerate 1,3-biphosphate 67-89 glucose-6-phosphate isomerase Homo sapiens 151-154 6460465-3 1981 The procedure is based on the conversion of glucose-6-phosphate to 1,3-diphosphoglycerate (1,3-DPG) which is catalyzed by the sequential action of the GPI, PFK, AL and GAPD. glycerate 1,3-biphosphate 91-98 glucose-6-phosphate isomerase Homo sapiens 151-154 7021949-0 1981 [Treatment of recent myocardial infarct with infusions of potassium, glucose and insulin (PGI). Potassium 58-67 glucose-6-phosphate isomerase Homo sapiens 90-93 24470082-0 1980 Oat leaf phosphoglucose isomerase: competitive inhibition by erythrose-4-phosphate. erythrose 4-phosphate 61-82 glucose-6-phosphate isomerase Homo sapiens 9-33 24470082-1 1980 Phosphoglucose isomerase was partially purified from oat leaves and shown to be strongly inhibited by erythrose-4-P. erythrose 4-phosphate 102-115 glucose-6-phosphate isomerase Homo sapiens 0-24 6249157-11 1980 Chloride efflux from several neurons appears to be involved in the regulation of intracellular pH (pHI). Chlorides 0-8 glucose-6-phosphate isomerase Homo sapiens 99-102 6249157-12 1980 When pHi is made acidic, chloride efflux from the squid giant axon increases and this stimulation requires cellular ATP and external HCO3-. Chlorides 25-33 glucose-6-phosphate isomerase Homo sapiens 5-8 6249157-12 1980 When pHi is made acidic, chloride efflux from the squid giant axon increases and this stimulation requires cellular ATP and external HCO3-. Adenosine Triphosphate 116-119 glucose-6-phosphate isomerase Homo sapiens 5-8 6249157-12 1980 When pHi is made acidic, chloride efflux from the squid giant axon increases and this stimulation requires cellular ATP and external HCO3-. Bicarbonates 133-138 glucose-6-phosphate isomerase Homo sapiens 5-8 6249157-13 1980 When pHi is measured following an acid load, it is found that pHi recovery toward normal values requires cellular Cl-, ATP and external HCO3-. Adenosine Triphosphate 119-122 glucose-6-phosphate isomerase Homo sapiens 5-8 6249157-13 1980 When pHi is measured following an acid load, it is found that pHi recovery toward normal values requires cellular Cl-, ATP and external HCO3-. Adenosine Triphosphate 119-122 glucose-6-phosphate isomerase Homo sapiens 62-65 6249157-13 1980 When pHi is measured following an acid load, it is found that pHi recovery toward normal values requires cellular Cl-, ATP and external HCO3-. Bicarbonates 136-141 glucose-6-phosphate isomerase Homo sapiens 5-8 6249157-13 1980 When pHi is measured following an acid load, it is found that pHi recovery toward normal values requires cellular Cl-, ATP and external HCO3-. Bicarbonates 136-141 glucose-6-phosphate isomerase Homo sapiens 62-65 157102-0 1979 Novel pronounced reversible inhibition of phosphofructokinase, glucose 6-phosphate dehydrogenase, and phosphoglucose isomerase by hexacyanoferrate (II). hexacyanoferrate II 130-146 glucose-6-phosphate isomerase Homo sapiens 102-126 438320-8 1979 GPI-deficient erythrocytes incubated with glucose in the medium showed an accentuation of membrane protein aggregate formation; however, this was almost completely reversed by the addition of adenine and inosine to the incubation medium or by the use of fructose, the intermediate just distal to the "block" in glycolysis, as the sole substrate. Glucose 42-49 glucose-6-phosphate isomerase Homo sapiens 0-3 438320-8 1979 GPI-deficient erythrocytes incubated with glucose in the medium showed an accentuation of membrane protein aggregate formation; however, this was almost completely reversed by the addition of adenine and inosine to the incubation medium or by the use of fructose, the intermediate just distal to the "block" in glycolysis, as the sole substrate. Adenine 193-200 glucose-6-phosphate isomerase Homo sapiens 0-3 438320-8 1979 GPI-deficient erythrocytes incubated with glucose in the medium showed an accentuation of membrane protein aggregate formation; however, this was almost completely reversed by the addition of adenine and inosine to the incubation medium or by the use of fructose, the intermediate just distal to the "block" in glycolysis, as the sole substrate. Inosine 205-212 glucose-6-phosphate isomerase Homo sapiens 0-3 438320-8 1979 GPI-deficient erythrocytes incubated with glucose in the medium showed an accentuation of membrane protein aggregate formation; however, this was almost completely reversed by the addition of adenine and inosine to the incubation medium or by the use of fructose, the intermediate just distal to the "block" in glycolysis, as the sole substrate. Fructose 255-263 glucose-6-phosphate isomerase Homo sapiens 0-3 26400-2 1978 Pyranine is shown to be a convenient and sensitive probe for reporting pH values, pHi, at the interior of anionic and at the outer surface of cationic liposomes. pyranine 0-8 glucose-6-phosphate isomerase Homo sapiens 82-85 438320-9 1979 ATP and reduced glutathione levels in the GPI-deficient erythrocytes incubated with glucose were similar to that found in the low and high reticulocyte controls. Adenosine Triphosphate 0-3 glucose-6-phosphate isomerase Homo sapiens 42-45 438320-9 1979 ATP and reduced glutathione levels in the GPI-deficient erythrocytes incubated with glucose were similar to that found in the low and high reticulocyte controls. Glutathione 16-27 glucose-6-phosphate isomerase Homo sapiens 42-45 438320-9 1979 ATP and reduced glutathione levels in the GPI-deficient erythrocytes incubated with glucose were similar to that found in the low and high reticulocyte controls. Glucose 84-91 glucose-6-phosphate isomerase Homo sapiens 42-45 35102-0 1979 Anomerization of glucose 6-phosphate: catalysis by phosphoglucose isomerase. Glucose-6-Phosphate 17-36 glucose-6-phosphate isomerase Homo sapiens 51-75 690596-6 1978 Rather, because alteration of intracellular pH (pHi) by using either (NH4)2SO4 or CO2 produced changes in the photoresponse similar to those caused by metabolic inhibition, it is suggested that changes in pHi during metabolic inhibition can account in part for the lengthening of the time scale. Ammonium Sulfate 69-78 glucose-6-phosphate isomerase Homo sapiens 48-51 690596-6 1978 Rather, because alteration of intracellular pH (pHi) by using either (NH4)2SO4 or CO2 produced changes in the photoresponse similar to those caused by metabolic inhibition, it is suggested that changes in pHi during metabolic inhibition can account in part for the lengthening of the time scale. Ammonium Sulfate 69-78 glucose-6-phosphate isomerase Homo sapiens 205-208 690596-6 1978 Rather, because alteration of intracellular pH (pHi) by using either (NH4)2SO4 or CO2 produced changes in the photoresponse similar to those caused by metabolic inhibition, it is suggested that changes in pHi during metabolic inhibition can account in part for the lengthening of the time scale. Carbon Dioxide 82-85 glucose-6-phosphate isomerase Homo sapiens 48-51 690596-6 1978 Rather, because alteration of intracellular pH (pHi) by using either (NH4)2SO4 or CO2 produced changes in the photoresponse similar to those caused by metabolic inhibition, it is suggested that changes in pHi during metabolic inhibition can account in part for the lengthening of the time scale. Carbon Dioxide 82-85 glucose-6-phosphate isomerase Homo sapiens 205-208 631848-4 1978 GPI "Paris" was characterized by a slow electrophoretic migration and, above all, a drastically altered affinity for the substrates glucose-6-phosphate (decreased) and fructose-6-phosphate (increased). Glucose-6-Phosphate 132-151 glucose-6-phosphate isomerase Homo sapiens 0-3 27853-1 1978 Experiments are reviewed in which intracellular pH (pHi), during exposure of a cell to CO2- or NH3-containing solutions, not only undergoes an acidification of alkalinization, respectively, but tends to return toward its original value and, upon removal of the test solution, rebounds to a value more alkaline or acid, respectively, than the initial one. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 87-90 glucose-6-phosphate isomerase Homo sapiens 52-55 27853-1 1978 Experiments are reviewed in which intracellular pH (pHi), during exposure of a cell to CO2- or NH3-containing solutions, not only undergoes an acidification of alkalinization, respectively, but tends to return toward its original value and, upon removal of the test solution, rebounds to a value more alkaline or acid, respectively, than the initial one. Ammonia 95-98 glucose-6-phosphate isomerase Homo sapiens 52-55 631848-4 1978 GPI "Paris" was characterized by a slow electrophoretic migration and, above all, a drastically altered affinity for the substrates glucose-6-phosphate (decreased) and fructose-6-phosphate (increased). fructose-6-phosphate 168-188 glucose-6-phosphate isomerase Homo sapiens 0-3 631848-5 1978 GPI "Enfants malades" exhibited a slightly reduced electrophoretic mobility, an abnormal curve of the activity in function of pH, and an abnormal ratio of maximal velocity in the backward direction (fructose-6-phosphate leads to glucose-6-phosphate) to that in the forward direction (glucose-6-phosphate leads to fructose-6-phosphate). fructose-6-phosphate 199-219 glucose-6-phosphate isomerase Homo sapiens 0-3 631848-5 1978 GPI "Enfants malades" exhibited a slightly reduced electrophoretic mobility, an abnormal curve of the activity in function of pH, and an abnormal ratio of maximal velocity in the backward direction (fructose-6-phosphate leads to glucose-6-phosphate) to that in the forward direction (glucose-6-phosphate leads to fructose-6-phosphate). Glucose-6-Phosphate 229-248 glucose-6-phosphate isomerase Homo sapiens 0-3 631848-5 1978 GPI "Enfants malades" exhibited a slightly reduced electrophoretic mobility, an abnormal curve of the activity in function of pH, and an abnormal ratio of maximal velocity in the backward direction (fructose-6-phosphate leads to glucose-6-phosphate) to that in the forward direction (glucose-6-phosphate leads to fructose-6-phosphate). Glucose-6-Phosphate 284-303 glucose-6-phosphate isomerase Homo sapiens 0-3 631848-5 1978 GPI "Enfants malades" exhibited a slightly reduced electrophoretic mobility, an abnormal curve of the activity in function of pH, and an abnormal ratio of maximal velocity in the backward direction (fructose-6-phosphate leads to glucose-6-phosphate) to that in the forward direction (glucose-6-phosphate leads to fructose-6-phosphate). fructose-6-phosphate 313-333 glucose-6-phosphate isomerase Homo sapiens 0-3 631848-7 1978 Finally we emphasized the possible role of the impairment of hexosemonophosphate pathway in the reduction of viability of the GPI-deficient red cells. hexosemonophosphate 61-80 glucose-6-phosphate isomerase Homo sapiens 126-129 23429-0 1977 The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones. Bicarbonates 12-23 glucose-6-phosphate isomerase Homo sapiens 85-87 747179-0 1978 A new mutant erythrocyte glucosephosphate isomerase (GPI) associated with GSH abnormality. Glutathione 74-77 glucose-6-phosphate isomerase Homo sapiens 25-51 747179-0 1978 A new mutant erythrocyte glucosephosphate isomerase (GPI) associated with GSH abnormality. Glutathione 74-77 glucose-6-phosphate isomerase Homo sapiens 53-56 618901-4 1978 The effect of p-fluorophenylalanine incorporation on PGI was examined in early passage fibroblasts. p-Fluorophenylalanine 14-35 glucose-6-phosphate isomerase Homo sapiens 53-56 23429-0 1977 The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones. Chlorides 25-33 glucose-6-phosphate isomerase Homo sapiens 85-87 23429-0 1977 The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones. Sodium 38-44 glucose-6-phosphate isomerase Homo sapiens 85-87 23429-3 1977 Reducing the external HCO(3) (-) concentration greatly inhibited the rate of pH(i) recovery from HCl injection.3. Bicarbonates 22-28 glucose-6-phosphate isomerase Homo sapiens 77-82 23429-3 1977 Reducing the external HCO(3) (-) concentration greatly inhibited the rate of pH(i) recovery from HCl injection.3. Hydrochloric Acid 97-100 glucose-6-phosphate isomerase Homo sapiens 77-82 23429-4 1977 Reducing external Cl(-) did not inhibit pH(i) recovery, but reducing internal Cl(-), by exposing the cell to sulphate Ringer, inhibited pH(i) recovery from CO(2) application.4. Sulfates 109-117 glucose-6-phosphate isomerase Homo sapiens 136-138 23429-4 1977 Reducing external Cl(-) did not inhibit pH(i) recovery, but reducing internal Cl(-), by exposing the cell to sulphate Ringer, inhibited pH(i) recovery from CO(2) application.4. co(2) 156-161 glucose-6-phosphate isomerase Homo sapiens 136-138 23429-5 1977 During pH(i) recovery from CO(2) application the internal Cl(-) concentration decreased. co(2) 27-32 glucose-6-phosphate isomerase Homo sapiens 7-9 23429-7 1977 Removal of external Na inhibited the pH(i) recovery from either CO(2) application or HCl injection.6. co(2) 64-69 glucose-6-phosphate isomerase Homo sapiens 37-39 23429-7 1977 Removal of external Na inhibited the pH(i) recovery from either CO(2) application or HCl injection.6. Hydrochloric Acid 85-88 glucose-6-phosphate isomerase Homo sapiens 37-39 23429-10 1977 The increase in [Na(+)](i) that occurred during pH(i) recovery from an injection of HCl was about half of that produced by a similar injection of NaCl.8. Hydrochloric Acid 84-87 glucose-6-phosphate isomerase Homo sapiens 48-50 23429-10 1977 The increase in [Na(+)](i) that occurred during pH(i) recovery from an injection of HCl was about half of that produced by a similar injection of NaCl.8. Sodium Chloride 146-150 glucose-6-phosphate isomerase Homo sapiens 48-50 23429-11 1977 The inhibitory effects of Na-free Ringer and of the anion exchange inhibitor SITS on pH(i) recovery after HCl injection were not additive.9. Hydrochloric Acid 106-109 glucose-6-phosphate isomerase Homo sapiens 85-87 23429-12 1977 It is concluded that the pH(i) regulating system involves tightly linked Cl(-)-HCO(3) (-) and Na(+)-H(+) exchange, with Na entry down its concentration gradient probably providing the energy to drive the movement inwards of HCO(3) (-) and the movement outward of Cl(-) and H(+) ions. 7 alpha-hydroxy-4-cholesten-3-one 79-82 glucose-6-phosphate isomerase Homo sapiens 25-27 23429-12 1977 It is concluded that the pH(i) regulating system involves tightly linked Cl(-)-HCO(3) (-) and Na(+)-H(+) exchange, with Na entry down its concentration gradient probably providing the energy to drive the movement inwards of HCO(3) (-) and the movement outward of Cl(-) and H(+) ions. 7 alpha-hydroxy-4-cholesten-3-one 224-227 glucose-6-phosphate isomerase Homo sapiens 25-27 21021-5 1977 NAE is significantly correlated with PaCO2, pHe, pHu and pHi in all the subjects considered together. N-lauroylethanolamine 0-3 glucose-6-phosphate isomerase Homo sapiens 57-60 912103-2 1977 The defective GPI is very thermolabile and migrates on starch gel electrophoresis as a single band with a mobility of 96% of the normal main band. Starch 55-61 glucose-6-phosphate isomerase Homo sapiens 14-17 14125-2 1976 The pHi"s thus obtained were found to be slightly lower (0.02-0.17 pH unit) than the pH"s of the external medium (pHe) at pH 6.5-8.5 in the presence of 105 mM KCl and 40 mM Tris-maleate buffer. Potassium Chloride 159-162 glucose-6-phosphate isomerase Homo sapiens 4-7 14803-5 1977 In chronic metabolic acidosis, pHi decreased in proportion to the total amount of ammonium chloride administered; pHi was normal in patients with uraemic acidosis. Ammonium Chloride 82-99 glucose-6-phosphate isomerase Homo sapiens 31-34 885551-1 1977 Six hundred individuals of pure Dutch ancestry have been typed for their erythrocyte phosphoglucose isomerase phenotypes by starch gel and cellulose acetate electrophoresis. Starch 124-130 glucose-6-phosphate isomerase Homo sapiens 85-109 14125-1 1976 The intravesicular pH (pHi) of fragmented sarcoplasmic reticulum (SR) of the skeletal muscle was determined from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO), a weak organic acid, between the intra- and extravesicular spaces. Dimethadione 133-166 glucose-6-phosphate isomerase Homo sapiens 23-26 14125-1 1976 The intravesicular pH (pHi) of fragmented sarcoplasmic reticulum (SR) of the skeletal muscle was determined from the distribution of 5,5-dimethyl-2,4-oxazolidinedione (DMO), a weak organic acid, between the intra- and extravesicular spaces. Dimethadione 168-171 glucose-6-phosphate isomerase Homo sapiens 23-26 14125-2 1976 The pHi"s thus obtained were found to be slightly lower (0.02-0.17 pH unit) than the pH"s of the external medium (pHe) at pH 6.5-8.5 in the presence of 105 mM KCl and 40 mM Tris-maleate buffer. Tris-maleate 173-185 glucose-6-phosphate isomerase Homo sapiens 4-7 14125-4 1976 When pHe was changed, pHi attained equilibrium within about 20 min, the time necessary for the separation of the SR by centrifugation. Phenylalanine 5-8 glucose-6-phosphate isomerase Homo sapiens 22-25 11849-2 1976 Under such conditions we evidenced that plasma pH (pHe) to erythrocyte pH (pHi) relationship is unchanged. Phenylalanine 51-54 glucose-6-phosphate isomerase Homo sapiens 47-49 4614-3 1976 The effects on pHi of CO2 applied externally and HCO3-, H+ and NH4+ injected iontophoretically, were investigated. Carbon Dioxide 22-25 glucose-6-phosphate isomerase Homo sapiens 15-18 13893-3 1976 Therefore, to estimate pHe-pHi relationship, pHi was evaluated on true erythrolysate. Phenylalanine 23-26 glucose-6-phosphate isomerase Homo sapiens 27-30 13893-4 1976 When haemoglobin passed from the reduced to the completely oxygenated state, a significant decrease of both pHe and pHi was observed for a given PCO2 (respectively about 0.05 and 0.08 pH unit), and of pHi for a given pHe (about 0.04 pH unit). pco2 145-149 glucose-6-phosphate isomerase Homo sapiens 116-119 13893-4 1976 When haemoglobin passed from the reduced to the completely oxygenated state, a significant decrease of both pHe and pHi was observed for a given PCO2 (respectively about 0.05 and 0.08 pH unit), and of pHi for a given pHe (about 0.04 pH unit). Phenylalanine 217-220 glucose-6-phosphate isomerase Homo sapiens 116-119 13893-4 1976 When haemoglobin passed from the reduced to the completely oxygenated state, a significant decrease of both pHe and pHi was observed for a given PCO2 (respectively about 0.05 and 0.08 pH unit), and of pHi for a given pHe (about 0.04 pH unit). Phenylalanine 217-220 glucose-6-phosphate isomerase Homo sapiens 201-204 4614-7 1976 Exposure to Ringer, pH 7-5, equilibrated with 2-2% CO2 caused a rapid, but only transient, fall in pHi. Carbon Dioxide 51-54 glucose-6-phosphate isomerase Homo sapiens 99-102 4614-10 1976 When external CO2 was removed, pHi rapidly increased, and then slowly returned to normal. Carbon Dioxide 14-17 glucose-6-phosphate isomerase Homo sapiens 31-34 4614-11 1976 The pHi changes with CO2 application or removal gave a calculated intracellular buffer value of about 30 m-equiv H+/pH unit per litre. Carbon Dioxide 21-24 glucose-6-phosphate isomerase Homo sapiens 4-7 4614-13 1976 Injection of HCO3- caused a rise in pHi very similar to that seen on removal of external CO2. Bicarbonates 13-17 glucose-6-phosphate isomerase Homo sapiens 36-39 4614-15 1976 The pHi responses to CO2 application, CO2 removal and HCO3- injection were slowed by the carbonic anhydrase inhibitor acetazolamide. Carbon Dioxide 21-24 glucose-6-phosphate isomerase Homo sapiens 4-7 4614-15 1976 The pHi responses to CO2 application, CO2 removal and HCO3- injection were slowed by the carbonic anhydrase inhibitor acetazolamide. Carbon Dioxide 38-41 glucose-6-phosphate isomerase Homo sapiens 4-7 4614-15 1976 The pHi responses to CO2 application, CO2 removal and HCO3- injection were slowed by the carbonic anhydrase inhibitor acetazolamide. Bicarbonates 54-58 glucose-6-phosphate isomerase Homo sapiens 4-7 4614-15 1976 The pHi responses to CO2 application, CO2 removal and HCO3- injection were slowed by the carbonic anhydrase inhibitor acetazolamide. Acetazolamide 118-131 glucose-6-phosphate isomerase Homo sapiens 4-7 4614-18 1976 In CO2 Ringer pHi fell less and recovered faster than in CO2-free Ringer. Carbon Dioxide 3-6 glucose-6-phosphate isomerase Homo sapiens 14-17 4614-19 1976 Calculation of the internal buffer value from the pHi responses to H+ and HCO3- injection gave very similar values. Bicarbonates 74-78 glucose-6-phosphate isomerase Homo sapiens 50-53 4614-24 1976 It was concluded that the internal HCO3- was determined primarily by the CO2 level and pHi, that internal HCO3- made a large contribution to the buffering power, and that after internal acidfication pHi was restored to normal by active transport of H+, OH- or HCO3- across the cell membrane. Bicarbonates 35-39 glucose-6-phosphate isomerase Homo sapiens 87-90 1164-3 1976 GP I Barcelona was a fast variant (116%) with an increased isoelectric point (9.55), lability to heat and to urea, and shift of the pH curve towards the acidic pH. Urea 109-113 glucose-6-phosphate isomerase Homo sapiens 0-4 1460-4 1976 If the exposure to CO2 was prolonged, two additional effects were noted: (a) during the exposure, the rapid initial fall in pHi was followed by a slow rise, and (b) after the exposure, the overshoot was greatly exaggerated. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 19-22 glucose-6-phosphate isomerase Homo sapiens 124-127 985736-6 1976 The presence of a human-specific enzyme identified by this method correlated with the presence of a particular human chromosome permitting assignments of the human cytoplasmic forms of NADP-linked IDH, human PGI, and human HGPRT genes to chromosomes 2, 19, and X, respectively. NADP 185-189 glucose-6-phosphate isomerase Homo sapiens 208-211 819039-2 1976 The purification procedure includes a two step precipitation by ammonium sulfate and one column chromatography on a cation exchanger with specific elution by 6 phosphogluconate, a ligand of GPI ; of the two cation exchangers tested, phosphocellulose was found to lead to a better yield than CM-Sephadex. 6-phosphogluconic acid 158-176 glucose-6-phosphate isomerase Homo sapiens 190-193 1460-5 1976 Application of external NH4Cl caused pHi to rise sharply; return to normal ASW caused pHi to return to a value below its initial one. Ammonium Chloride 24-29 glucose-6-phosphate isomerase Homo sapiens 37-40 1460-3 1976 Exposure of the axon to 5% CO2 at constant external pH caused a sharp decrease in pHi, while the subsequent removal of the gas caused pHi to overshoot its initial value. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 27-30 glucose-6-phosphate isomerase Homo sapiens 82-85 1460-5 1976 Application of external NH4Cl caused pHi to rise sharply; return to normal ASW caused pHi to return to a value below its initial one. Ammonium Chloride 24-29 glucose-6-phosphate isomerase Homo sapiens 86-89 1460-6 1976 If the exposure to NH4Cl was prolonged, two additional effects were noted: (a) during the exposure, the rapid initial rise in pHi was followed by a slow fall, and (b) after the exposure, the undershoot was greatly exaggerated. Ammonium Chloride 19-24 glucose-6-phosphate isomerase Homo sapiens 126-129 1460-9 1976 A mathematical model explains the pHi changes caused by NH4Cl on the basis of passive movements of both NH3 and NH4+. Ammonium Chloride 56-61 glucose-6-phosphate isomerase Homo sapiens 34-37 1460-9 1976 A mathematical model explains the pHi changes caused by NH4Cl on the basis of passive movements of both NH3 and NH4+. Ammonia 104-107 glucose-6-phosphate isomerase Homo sapiens 34-37 1460-9 1976 A mathematical model explains the pHi changes caused by NH4Cl on the basis of passive movements of both NH3 and NH4+. Ammonium Compounds 112-116 glucose-6-phosphate isomerase Homo sapiens 34-37 1227349-1 1975 The authors describe simple and rapid separation technics by electrophoresis on cellulose acetate of glucose-6-phosphate dehydrogenase isoenzymes, 6-phosphogluconate dehydrogenase, phosphoglucomutase, adenosine deaminase, adenylate kinase, phosphohexose isomerase, lactate dehydrogenase iosenzymes in the red cells. acetylcellulose 80-97 glucose-6-phosphate isomerase Homo sapiens 201-263 240822-0 1975 Mechanistic implications of the pH independence of inhibition of phosphoglucose isomerase by neutral sugar phosphates. Sugar Phosphates 101-117 glucose-6-phosphate isomerase Homo sapiens 65-89 240822-4 1975 The pH independence of inhibition by erythrose 4-phosphate and other neutral sugar phosphates may reflect a mode and locus of binding to phosphoglucose isomerase different from that of the aldonate inhibitors. erythrose 4-phosphate 37-58 glucose-6-phosphate isomerase Homo sapiens 137-161 240822-4 1975 The pH independence of inhibition by erythrose 4-phosphate and other neutral sugar phosphates may reflect a mode and locus of binding to phosphoglucose isomerase different from that of the aldonate inhibitors. Sugars 77-82 glucose-6-phosphate isomerase Homo sapiens 137-161 240822-4 1975 The pH independence of inhibition by erythrose 4-phosphate and other neutral sugar phosphates may reflect a mode and locus of binding to phosphoglucose isomerase different from that of the aldonate inhibitors. Phosphates 83-93 glucose-6-phosphate isomerase Homo sapiens 137-161 5407-0 1975 Intracellular pH (pHi) of red cells stored in acid citrate dextrose medium. acid citrate dextrose 46-67 glucose-6-phosphate isomerase Homo sapiens 18-21 5407-2 1975 The intracellular pH (pHi) of red cells stored in acid citrate dextrose (ACD) medium was estimated by the 5,5"-dimethyloxazoldine,-2,4-dione (DMO) method. acid citrate dextrose 50-71 glucose-6-phosphate isomerase Homo sapiens 22-25 5407-2 1975 The intracellular pH (pHi) of red cells stored in acid citrate dextrose (ACD) medium was estimated by the 5,5"-dimethyloxazoldine,-2,4-dione (DMO) method. acid citrate dextrose 73-76 glucose-6-phosphate isomerase Homo sapiens 22-25 5407-2 1975 The intracellular pH (pHi) of red cells stored in acid citrate dextrose (ACD) medium was estimated by the 5,5"-dimethyloxazoldine,-2,4-dione (DMO) method. 5,5"-dimethyloxazoldine,-2,4-dione 106-140 glucose-6-phosphate isomerase Homo sapiens 22-25 5407-2 1975 The intracellular pH (pHi) of red cells stored in acid citrate dextrose (ACD) medium was estimated by the 5,5"-dimethyloxazoldine,-2,4-dione (DMO) method. demethyloleuropein 142-145 glucose-6-phosphate isomerase Homo sapiens 22-25 5407-5 1975 The high pHi of ACD blood was a results of the temperature at which the pHe and the pHi were measured (4degrees) and the presence of citrate anions in the medium, and could be explained by application of the Donnan-Gibbs equilibrium. Phenylalanine 72-75 glucose-6-phosphate isomerase Homo sapiens 9-12 5407-5 1975 The high pHi of ACD blood was a results of the temperature at which the pHe and the pHi were measured (4degrees) and the presence of citrate anions in the medium, and could be explained by application of the Donnan-Gibbs equilibrium. Citric Acid 133-140 glucose-6-phosphate isomerase Homo sapiens 9-12 5407-6 1975 ATP and 2,3-diphosphoglycerate (DPG) were well-maintained in heparinized blood when it was acidified and pHe and pHi at 4degrees were both about 7.4, which suggests that improvement of blood preservation may be attained by suitable adjustment of the pHi and pHe of the blood. Adenosine Triphosphate 0-3 glucose-6-phosphate isomerase Homo sapiens 113-116 5407-6 1975 ATP and 2,3-diphosphoglycerate (DPG) were well-maintained in heparinized blood when it was acidified and pHe and pHi at 4degrees were both about 7.4, which suggests that improvement of blood preservation may be attained by suitable adjustment of the pHi and pHe of the blood. Adenosine Triphosphate 0-3 glucose-6-phosphate isomerase Homo sapiens 250-253 5407-6 1975 ATP and 2,3-diphosphoglycerate (DPG) were well-maintained in heparinized blood when it was acidified and pHe and pHi at 4degrees were both about 7.4, which suggests that improvement of blood preservation may be attained by suitable adjustment of the pHi and pHe of the blood. 2,3-Diphosphoglycerate 32-35 glucose-6-phosphate isomerase Homo sapiens 250-253 1070710-1 1976 SDS-polyacrylamide gel electrophoresis of solubilized whole platelets and isolated platelet membranes from patients with chronic myeloid leukemia (CML) as compared to the normal platelet-Schiff-stained glycoproteins (GP) (noted GP I, II, III of app. Sodium Dodecyl Sulfate 0-3 glucose-6-phosphate isomerase Homo sapiens 228-232 1222331-10 1975 The DPG-pHi correlation is very good (r = 0.691 ; pless 0.001) and the changes of the acid-base balance seem to be the main factors for controlling the DPG synthesis : it increases it in chronic alkalosis and reduces it in chronic acidosis. 2,3-Diphosphoglycerate 4-7 glucose-6-phosphate isomerase Homo sapiens 8-11 4140968-0 1974 [Colorimetric method of determination of phosphohexose isomerase with the aid of anthrone reagent]. anthrone 81-89 glucose-6-phosphate isomerase Homo sapiens 41-64 13954993-1 1963 A simple, rapid colorimetric method for the estimation of phosphoglucose isomerase activity, using the determination of fructose by the method of Roe, Epstein, and Goldstein (1949), is described. Fructose 120-128 glucose-6-phosphate isomerase Homo sapiens 58-82 5421254-0 1970 [Automatic determination of phosphohexose isomerase by the reaction to thiobarbituric acid]. thiobarbituric acid 71-90 glucose-6-phosphate isomerase Homo sapiens 28-51 5722340-0 1968 [Colorimetric determination of phosphohexose isomerase by thiobarbituric acid reaction]. thiobarbituric acid 58-77 glucose-6-phosphate isomerase Homo sapiens 31-54 4570473-0 1973 Mannose 6-phosphate: anomeric form used by phosphomannose isomerase and its 1-epimerization by phosphoglucose isomerase. mannose-6-phosphate 0-19 glucose-6-phosphate isomerase Homo sapiens 95-119 5141677-0 1971 Effect of phosphoglucose isomerase and glucose 6-phosphate on UDP-N-acetylglucosamine inhibition of L-glutamine-D-fructose 6-phosphate aminotransferase. Uridine Diphosphate N-Acetylglucosamine 62-85 glucose-6-phosphate isomerase Homo sapiens 10-34 33508621-4 2021 By exchanging H+ and K+ across cell membranes, nigericin shows promising anti-cancer activities in in vitro and in vivo as a single agent or in combination with other anti-cancer drugs through decreasing intracellular pH (pHi). Nigericin 47-56 glucose-6-phosphate isomerase Homo sapiens 222-225 34038242-4 2021 Using the pharmacological reagent ethyl isopropyl amiloride (EIPA) to inhibit NHE1 activity and decrease pHi, we observe no change in glycolysis, as indicated by secreted lactate and intracellular pyruvate, despite confirming increased activity of the glycolytic enzyme phosphofructokinase-1 at higher pH. ethylisopropylamiloride 34-59 glucose-6-phosphate isomerase Homo sapiens 105-108 34038242-4 2021 Using the pharmacological reagent ethyl isopropyl amiloride (EIPA) to inhibit NHE1 activity and decrease pHi, we observe no change in glycolysis, as indicated by secreted lactate and intracellular pyruvate, despite confirming increased activity of the glycolytic enzyme phosphofructokinase-1 at higher pH. ethylisopropylamiloride 61-65 glucose-6-phosphate isomerase Homo sapiens 105-108 32423271-8 2021 The majority felt there was still a role for GPI in accordance to the American College of Cardiology (ACC)/American Heart Association (AHA) guidelines for ST-segment elevation myocardial infarction (65.2%), with eptifibatide being preferred (55.1%). Eptifibatide 212-224 glucose-6-phosphate isomerase Homo sapiens 45-48 33556676-3 2021 CD offspring had significantly lower drip loss and higher pHi, intramuscular fat content than BP+ and BP- (except for pHi). Cadmium 0-2 glucose-6-phosphate isomerase Homo sapiens 58-61 33380437-8 2022 The AAG group had lower pepsinogen (PG) I and II levels, as well as a lower PGI/PGII ratio, compared with the EAG group (p<0.01, p<0.05 and p<0.01, respectively). aag 4-7 glucose-6-phosphate isomerase Homo sapiens 76-79 33824463-4 2021 Longitudinal studies of seven patients in Group A showed a gradual decrease in the percentage of HLA(-) granulocytes, with a reciprocal increase in the GPI(-) granulocytes in four patients responding to cyclosporine (CsA) and an increase in the HLA(-) granulocytes with a stable or declining GPI(-) granulocytes in three patients in sustained remission off CsA therapy. Cyclosporine 203-215 glucose-6-phosphate isomerase Homo sapiens 152-155 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. nh3-n 40-45 glucose-6-phosphate isomerase Homo sapiens 97-100 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. po43--p 50-57 glucose-6-phosphate isomerase Homo sapiens 97-100 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. nh3-n 117-122 glucose-6-phosphate isomerase Homo sapiens 97-100 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. po43--p 127-134 glucose-6-phosphate isomerase Homo sapiens 97-100 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. magnesium ion 175-179 glucose-6-phosphate isomerase Homo sapiens 97-100 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. magnesium ion 207-211 glucose-6-phosphate isomerase Homo sapiens 97-100 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. Ammonium Compounds 212-216 glucose-6-phosphate isomerase Homo sapiens 97-100 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. magnesium ion 207-211 glucose-6-phosphate isomerase Homo sapiens 97-100 33383251-5 2021 Optimization of NRE revealed that > 90% NH3-N and PO43--P could be recovered at 8 mA cm-2 with a pHi of 6-8. Notable NH3-N and PO43--P reduction were observed at an equimolar Mg2+ dissolution ratio (1:1) of Mg2+:NH4+ and a 1.1:1 ratio of Mg2+:PO43- respectively. po43 50-54 glucose-6-phosphate isomerase Homo sapiens 97-100 33160662-4 2021 The obtained results suggested that the critical genes involved in the carbohydrate and protein metabolisms (i.e. pgmB, GPI, glsA, pyrB and etc.) Carbohydrates 71-83 glucose-6-phosphate isomerase Homo sapiens 120-123 33635336-7 2021 Variations in pHi have a remarkable impact on [Ca2+]i and hence on some of the basic biochemical mechanisms of calcium signaling. Calcium 111-118 glucose-6-phosphate isomerase Homo sapiens 14-17 33586851-4 2021 We synthesized a series of GPI fragments and evaluated the interactions and arrangement of these glycolipids in monolayers as a 2-D membrane model. Glycolipids 97-108 glucose-6-phosphate isomerase Homo sapiens 27-30 32841374-4 2021 pHi was detected by microspectrofluorimetry with pH-sensitive dye-BCECF. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 66-71 glucose-6-phosphate isomerase Homo sapiens 0-3 33510857-10 2021 Subsequently, the model predicts the effects of six selected drug targets, such as, the deactivation of transketolase and glucose-6-phosphate isomerase among others, in the case of mammalian malignant cells in terms of growth, proliferation, fermentation, and energy supply in the form of adenosine triphosphate (ATP). Adenosine 289-298 glucose-6-phosphate isomerase Homo sapiens 122-151 33510857-10 2021 Subsequently, the model predicts the effects of six selected drug targets, such as, the deactivation of transketolase and glucose-6-phosphate isomerase among others, in the case of mammalian malignant cells in terms of growth, proliferation, fermentation, and energy supply in the form of adenosine triphosphate (ATP). Adenosine Triphosphate 313-316 glucose-6-phosphate isomerase Homo sapiens 122-151 33380437-9 2022 The optimal PGI/PGII ratio for predicting AAG was <=1.9 (100% sensitivity and 100% specificity), and that for predicting EAG was <=9.2 (47.5% sensitivity and 90.6% specificity). aag 42-45 glucose-6-phosphate isomerase Homo sapiens 12-15 33101887-4 2020 Thus, AMF/GPI and AMFR/gp78 contribute to higher metabolic turnover of protein and glucose. Glucose 83-90 glucose-6-phosphate isomerase Homo sapiens 6-9 33042728-3 2020 Their photophysical properties show that the axial substitution results into a relatively higher fluorescence quantum efficiency (Phi F=5.74 for m-hydroxybenzoic acid and 9.09 % for m-hydroxyphenylacetic acid) in comparison with that of the prototype compound, despite the almost negligible influence on the maximum absorption or the emission position. 3-hydroxybenzoic acid 145-166 glucose-6-phosphate isomerase Homo sapiens 130-133 31415279-8 2020 She was found to be compound heterozygous for 2 novel missense mutations, c.490C>A p.(Pro164Thr) and c.817C>T p.(Arg273Cys), in the GPI gene. pro164thr 86-95 glucose-6-phosphate isomerase Homo sapiens 132-135 33093276-1 2020 TWIK-related two-pore domain K+ channel-2 (TREK-2) has voltageindependent activity and shows additional activation by acidic intracellular pH (pHi) via neutralizing the E332 in the cytoplasmic C terminal (Ct). Potassium Citrate 169-173 glucose-6-phosphate isomerase Homo sapiens 143-146 33093276-8 2020 Also, the inhibitory effect of ATP could be commonly demonstrated under the activation by acidic pHi in R355-7A, G334A/R355-7A, and G334A/R355-7A/R377-9A. Adenosine Triphosphate 31-34 glucose-6-phosphate isomerase Homo sapiens 97-100 33659434-3 2020 Herein, we describe a protocol for pHi measurement in living oocytes microinjected with the pH-sensitive dye BCECF. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 109-114 glucose-6-phosphate isomerase Homo sapiens 35-38 33659434-5 2020 Of note, if the actual pHi is higher or lower than the known pH of injected standard solutions, the BCECF fluorescence intensity ratio will decrease or increase, respectively. 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein 100-105 glucose-6-phosphate isomerase Homo sapiens 23-26 32514887-9 2020 RESULTS: Hyperpolarized 1-13C-pyruvate MRS revealed a high rate of lactate production immediately at day 1 after GPI-serum transfer, which remained elevated during the progression of the disease, while 19F-MRI exhibited a gradual recruitment of phagocytic immune cells in arthritic ankles, which correlated well with the course of ankle swelling. Lactic Acid 67-74 glucose-6-phosphate isomerase Homo sapiens 113-116 33101887-4 2020 Thus, AMF/GPI and AMFR/gp78 contribute to higher metabolic turnover of protein and glucose. Glucose 83-90 glucose-6-phosphate isomerase Homo sapiens 10-13 32983978-3 2020 Hypoxia-induced carbonic anhydrase IX (CA IX) plays a critical adaptive role in response to hypoxic and acidic environments by catalytically hydrating extracellular CO2 to produce bicarbonate for buffering intracellular pH (pHi). Carbon Dioxide 165-168 glucose-6-phosphate isomerase Homo sapiens 224-227 33042622-9 2020 GPI was found to be downstream effector of HP while knockdown of GPI led to decreased glycolytic activity and restored oxygen consumption. Oxygen 119-125 glucose-6-phosphate isomerase Homo sapiens 65-68 32819571-6 2020 In addition, elevated blood PGI in NASH patients coincided with increased blood L-lactate. L-lactate 80-89 glucose-6-phosphate isomerase Homo sapiens 28-31 32819571-7 2020 Cell culture experiments were then conducted to delineate the PGI-lactate axis, which revealed that treatment of HepG2 cells with recombinant PGI protein stimulated glycolysis and lactate output, suggesting that the disease-induced PGI likely contributed to the increased lactate in NASH patients. Lactic Acid 66-73 glucose-6-phosphate isomerase Homo sapiens 62-65 32819571-7 2020 Cell culture experiments were then conducted to delineate the PGI-lactate axis, which revealed that treatment of HepG2 cells with recombinant PGI protein stimulated glycolysis and lactate output, suggesting that the disease-induced PGI likely contributed to the increased lactate in NASH patients. Lactic Acid 66-73 glucose-6-phosphate isomerase Homo sapiens 142-145 32819571-7 2020 Cell culture experiments were then conducted to delineate the PGI-lactate axis, which revealed that treatment of HepG2 cells with recombinant PGI protein stimulated glycolysis and lactate output, suggesting that the disease-induced PGI likely contributed to the increased lactate in NASH patients. Lactic Acid 66-73 glucose-6-phosphate isomerase Homo sapiens 142-145 32819571-7 2020 Cell culture experiments were then conducted to delineate the PGI-lactate axis, which revealed that treatment of HepG2 cells with recombinant PGI protein stimulated glycolysis and lactate output, suggesting that the disease-induced PGI likely contributed to the increased lactate in NASH patients. Lactic Acid 180-187 glucose-6-phosphate isomerase Homo sapiens 142-145 32819571-7 2020 Cell culture experiments were then conducted to delineate the PGI-lactate axis, which revealed that treatment of HepG2 cells with recombinant PGI protein stimulated glycolysis and lactate output, suggesting that the disease-induced PGI likely contributed to the increased lactate in NASH patients. Lactic Acid 180-187 glucose-6-phosphate isomerase Homo sapiens 142-145 32983978-7 2020 Interestingly, addition of the alternative LDH substrate alpha-ketobutyrate restored LDHA activity, extracellular acidification, pHi, and cellular proliferation. acetoacetic acid 57-75 glucose-6-phosphate isomerase Homo sapiens 129-132 32983978-8 2020 These results indicate that in the absence of CA IX, reduction of pHi disrupts LDHA activity and hinders the cellular capacity to regenerate NAD+ and secrete protons to the extracellular space. NAD 141-145 glucose-6-phosphate isomerase Homo sapiens 66-69 32983978-3 2020 Hypoxia-induced carbonic anhydrase IX (CA IX) plays a critical adaptive role in response to hypoxic and acidic environments by catalytically hydrating extracellular CO2 to produce bicarbonate for buffering intracellular pH (pHi). Bicarbonates 180-191 glucose-6-phosphate isomerase Homo sapiens 224-227 32498834-7 2020 This biosensor uses a synthetic GPI phosphoglycan bioreceptor immobilized on screen-printed gold electrodes through a linear alkane thiol phosphodiester. Sulfhydryl Compounds 132-137 glucose-6-phosphate isomerase Homo sapiens 32-35 32682158-1 2020 Phosphoglucose isomerase (PGI) is a cytosolic enzyme that catalyzes the reversible interconversion of d-glucose 6-phosphate and d-fructose 6-phosphate in glycolysis. Glucose 7-14 glucose-6-phosphate isomerase Homo sapiens 26-29 32682158-1 2020 Phosphoglucose isomerase (PGI) is a cytosolic enzyme that catalyzes the reversible interconversion of d-glucose 6-phosphate and d-fructose 6-phosphate in glycolysis. fructose-6-phosphate 128-150 glucose-6-phosphate isomerase Homo sapiens 0-24 32682158-1 2020 Phosphoglucose isomerase (PGI) is a cytosolic enzyme that catalyzes the reversible interconversion of d-glucose 6-phosphate and d-fructose 6-phosphate in glycolysis. fructose-6-phosphate 128-150 glucose-6-phosphate isomerase Homo sapiens 26-29 32682158-4 2020 Hence, with the purpose of finding new strong AMF-PGI inhibitors that could be potentially used as anticancer agents and/or as bioreceptors for carbohydrate-based electrochemical biosensors, we report in this study the synthesis and kinetic evaluation of several new human PGI inhibitors derived from the synthon 5-phospho-d-arabinono-1,4-lactone. Carbohydrates 144-156 glucose-6-phosphate isomerase Homo sapiens 50-53 32682158-4 2020 Hence, with the purpose of finding new strong AMF-PGI inhibitors that could be potentially used as anticancer agents and/or as bioreceptors for carbohydrate-based electrochemical biosensors, we report in this study the synthesis and kinetic evaluation of several new human PGI inhibitors derived from the synthon 5-phospho-d-arabinono-1,4-lactone. (+)-Synthon 305-312 glucose-6-phosphate isomerase Homo sapiens 50-53 32682158-4 2020 Hence, with the purpose of finding new strong AMF-PGI inhibitors that could be potentially used as anticancer agents and/or as bioreceptors for carbohydrate-based electrochemical biosensors, we report in this study the synthesis and kinetic evaluation of several new human PGI inhibitors derived from the synthon 5-phospho-d-arabinono-1,4-lactone. 5-phospho-d-arabinono-1,4-lactone 313-346 glucose-6-phosphate isomerase Homo sapiens 50-53 32682158-5 2020 Although not designed as high-energy intermediate analogue inhibitors of the enzyme catalyzed isomerization reaction, several of these N-substituted 5-phosphate-d-arabinonamide derivatives appears as new strong PGI inhibitors. n-substituted 5-phosphate-d-arabinonamide 135-176 glucose-6-phosphate isomerase Homo sapiens 211-214 32682158-7 2020 Detailed analysis of its interactions at the active site reveals a new binding mode and shows that human PGI is relatively tolerant for modified inhibitors at the "head" C-1 part, offering promising perspectives for the future design of carbohydrate-based biosensors. Carbohydrates 237-249 glucose-6-phosphate isomerase Homo sapiens 105-108 33132561-0 2020 "PGI Score": A Simplified Three-point Prognostic Score for Acute Aluminum Phosphide Poisoning. aluminum phosphide 65-83 glucose-6-phosphate isomerase Homo sapiens 1-4 33132561-9 2020 How to cite this article: Pannu AK, Bhalla A, Sharma A, Sharma N. "PGI Score": A Simplified Three-point Prognostic Score for Acute Aluminum Phosphide Poisoning. aluminum phosphide 131-149 glucose-6-phosphate isomerase Homo sapiens 67-70 32672252-7 2020 This enabled the application of a pH sensitive oligoyne compound to the ratiometric sensing of pHi in prostate cancer (PC3) cells in response to drug treatment. oligoyne 47-55 glucose-6-phosphate isomerase Homo sapiens 95-98 32672252-8 2020 We propose that probes based on Alkyne Tag Raman Imaging offer an entirely new platform for the sensing of pHi, complementary to fluorescence microscopy. Alkynes 32-38 glucose-6-phosphate isomerase Homo sapiens 107-110 32200349-2 2020 This boost in glycolytic flux supports proliferation, but also generates acid in the form of hydrogen ions that must be eliminated from the cytoplasm to maintain the alkaline intracellular pH (pHi) associated with transformation. Hydrogen 93-101 glucose-6-phosphate isomerase Homo sapiens 193-196 32669967-6 2020 Further, acidic pHi could activate the ubiquitin-proteasome system and inhibiting proteasome activity by MG132 prevented cells entering quiescence. benzyloxycarbonylleucyl-leucyl-leucine aldehyde 105-110 glucose-6-phosphate isomerase Homo sapiens 16-19 32542257-8 2020 We monitored the pHi change of individual HeLa and fibroblast cells, triggered by the extracellular pH (pHe) change. Phenylalanine 104-107 glucose-6-phosphate isomerase Homo sapiens 17-20 32079672-4 2020 We searched PubMed for studies on genetically confirmed monogenic dystonia treated with GPi DBS documenting pre-surgical and post-surgical assessments using the Burke-Fahn-Marsden Dystonia Rating Scale Motor Score (BFMMS) and Burke-Fahn-Marsden Disability Score (BFMDS). dibromsalan 92-95 glucose-6-phosphate isomerase Homo sapiens 88-91 31784059-6 2020 It was found that FAS bound strongly to Glucose-6-phosphate isomerase (GPI), and that scopolin could affect this interaction by proteomics and MST. scopolin 86-94 glucose-6-phosphate isomerase Homo sapiens 40-69 31784059-6 2020 It was found that FAS bound strongly to Glucose-6-phosphate isomerase (GPI), and that scopolin could affect this interaction by proteomics and MST. scopolin 86-94 glucose-6-phosphate isomerase Homo sapiens 71-74 31784059-7 2020 The results of this study demonstrate that the active compounds in Cortex Fraxini could play an anti-tumor role by binding to FAS and inhibiting the interactions between FAS and GPI to affect glucose and lipid metabolism, and that the protein pathway is a potential novel target for tumor treatment. Glucose 192-199 glucose-6-phosphate isomerase Homo sapiens 178-181 32130622-1 2020 Mammalian Na+/H+ exchanger type I isoform (NHE1) is a ubiquitously expressed membrane protein that regulates intracellular pH (pHi) by removing one intracellular proton in exchange for one extracellular sodium ion. Sodium 203-209 glucose-6-phosphate isomerase Homo sapiens 127-130 32187052-10 2020 Compared with those for patients receiving MTX monotherapy at baseline, the GPI levels were significantly declined when MTX was combined with infliximab (P < 0.001). Methotrexate 43-46 glucose-6-phosphate isomerase Homo sapiens 76-79 32187052-10 2020 Compared with those for patients receiving MTX monotherapy at baseline, the GPI levels were significantly declined when MTX was combined with infliximab (P < 0.001). Methotrexate 120-123 glucose-6-phosphate isomerase Homo sapiens 76-79 32079672-10 2020 CONCLUSIONS: GPi DBS outcomes vary across monogenic dystonias. dibromsalan 17-20 glucose-6-phosphate isomerase Homo sapiens 13-16 32156170-3 2020 The GPI consists of the conserved core glycan, phosphatidylinositol and glycan side chains. Polysaccharides 39-45 glucose-6-phosphate isomerase Homo sapiens 4-7 31909840-0 2020 Calibration of the dianionic phosphate group: Validation on the recognition site of the homodimeric enzyme phosphoglucose isomerase. Phosphates 29-38 glucose-6-phosphate isomerase Homo sapiens 107-131 31909840-3 2020 It is a constitutive fragment of biosensors, which bind to the dimer interface of phosphoglucose isomerase (PGI), an intracellular enzyme involved in sugar metabolism, as well as an extracellular protein known as autocrine motility factor (AMF) closely related to metastasis formation. Carbohydrates 150-155 glucose-6-phosphate isomerase Homo sapiens 82-106 31909840-3 2020 It is a constitutive fragment of biosensors, which bind to the dimer interface of phosphoglucose isomerase (PGI), an intracellular enzyme involved in sugar metabolism, as well as an extracellular protein known as autocrine motility factor (AMF) closely related to metastasis formation. Carbohydrates 150-155 glucose-6-phosphate isomerase Homo sapiens 108-111 31909840-3 2020 It is a constitutive fragment of biosensors, which bind to the dimer interface of phosphoglucose isomerase (PGI), an intracellular enzyme involved in sugar metabolism, as well as an extracellular protein known as autocrine motility factor (AMF) closely related to metastasis formation. Carbohydrates 150-155 glucose-6-phosphate isomerase Homo sapiens 240-243 31922299-4 2020 METHODS: Two groups of patients with focal or segmental dystonia were included in the study: 6 patients with GPi-DBS and 8 without DBS (control group). dibromsalan 113-116 glucose-6-phosphate isomerase Homo sapiens 109-112 31922299-7 2020 RESULTS: In patients with GPi-DBS (stimulation ON and OFF), activity at rest was reduced in a prefrontal network, and during the motor task, sensorimotor cortex activity was lower than in patients without DBS. dibromsalan 30-33 glucose-6-phosphate isomerase Homo sapiens 26-29 31922299-8 2020 Within-group contrasts (tapping > rest) showed less extensive task-induced motor network activation in GPi-DBS patients than in non-DBS controls. dibromsalan 107-110 glucose-6-phosphate isomerase Homo sapiens 103-106 31874233-7 2020 Changes in pHi recovery due to intracellular acidification and alkalization induced by NH4Cl prepulse and Na-acetate prepulse, respectively, were detected by microspectrofluorimetry with the pH-sensitive fluorescent dye BCECF. Ammonium Chloride 87-92 glucose-6-phosphate isomerase Homo sapiens 11-14 31874233-7 2020 Changes in pHi recovery due to intracellular acidification and alkalization induced by NH4Cl prepulse and Na-acetate prepulse, respectively, were detected by microspectrofluorimetry with the pH-sensitive fluorescent dye BCECF. Sodium 106-116 glucose-6-phosphate isomerase Homo sapiens 11-14 32292350-0 2020 Esculetin Inhibits Cancer Cell Glycolysis by Binding Tumor PGK2, GPD2, and GPI. esculetin 0-9 glucose-6-phosphate isomerase Homo sapiens 75-78 32292350-8 2020 Animal tests have further demonstrated that esculetin may have anticancer effects by affecting the activity of PGK2, GPD2, and GPI. esculetin 44-53 glucose-6-phosphate isomerase Homo sapiens 127-130 32156170-3 2020 The GPI consists of the conserved core glycan, phosphatidylinositol and glycan side chains. Polysaccharides 72-78 glucose-6-phosphate isomerase Homo sapiens 4-7 32156170-3 2020 The GPI consists of the conserved core glycan, phosphatidylinositol and glycan side chains. Phosphatidylinositols 47-67 glucose-6-phosphate isomerase Homo sapiens 4-7 32156170-4 2020 The entire GPI-AP is anchored to the outer leaflet of the lipid bilayer by insertion of fatty chains of phosphatidylinositol. Phosphatidylinositols 104-124 glucose-6-phosphate isomerase Homo sapiens 11-14 31932506-4 2020 The observed localized pHi increases require the initial uptake of HCO3 -, which is mediated by several proteins acting consistently with their subcellular localization. ferrous bicarbonate 67-71 glucose-6-phosphate isomerase Homo sapiens 23-26 31691827-8 2020 RESULTS: In the maintenance phase, solriamfetol-treated participants demonstrated clinically meaningful improvements on ESS, PGI-C, and CGI-C. solriamfetol 35-47 glucose-6-phosphate isomerase Homo sapiens 125-128 31930891-8 2020 CONCLUSION: The mild moxibustion combined with salt-separated moxibustion could effectively improve the symptoms of nausea, vomiting and constipation caused by chemotherapy in patients with breast cancer, and its mechanism may be related to the down-regulation of the levels of PGI, PGII and G-17 in serum. Salts 47-51 glucose-6-phosphate isomerase Homo sapiens 278-281 31698999-0 2020 Glucose 6-Phosphate Accumulates via Phosphoglucose Isomerase Inhibition in Heart Muscle. Glucose-6-Phosphate 0-19 glucose-6-phosphate isomerase Homo sapiens 36-60 31698999-5 2020 Metabolic control analysis revealed that glucose 6-phosphate concentration is dependent on phosphoglucose isomerase activity. Glucose-6-Phosphate 41-60 glucose-6-phosphate isomerase Homo sapiens 91-115 31698999-9 2020 Inhibition of glycolytic flux at the level of phosphoglucose isomerase caused glucose 6-phosphate accumulation, which correlated with increased mTOR activation. Phosphates 86-97 glucose-6-phosphate isomerase Homo sapiens 46-70 31918564-1 2020 Aggressive tumor cells mainly rely on glycolysis, and further release vast amounts of lactate and protons by monocarboxylate transporter (MCT), which causes a higher intracellular pH (pHi) and acidic extracellular pH. Lactic Acid 86-93 glucose-6-phosphate isomerase Homo sapiens 184-187 31918564-1 2020 Aggressive tumor cells mainly rely on glycolysis, and further release vast amounts of lactate and protons by monocarboxylate transporter (MCT), which causes a higher intracellular pH (pHi) and acidic extracellular pH. dimethoxy biphenyl monocarboxylate 109-124 glucose-6-phosphate isomerase Homo sapiens 184-187 31803912-1 2020 Our previous postmortem studies on neonates with neuropathological injury of perinatal hypoxia/ischemia (PHI) showed a dramatic reduction of tyrosine hydroxylase expression (dopamine synthesis enzyme) in substantia nigra (SN) neurons, with reduction of their cellular size. Tyrosine 141-149 glucose-6-phosphate isomerase Homo sapiens 77-109 31803912-1 2020 Our previous postmortem studies on neonates with neuropathological injury of perinatal hypoxia/ischemia (PHI) showed a dramatic reduction of tyrosine hydroxylase expression (dopamine synthesis enzyme) in substantia nigra (SN) neurons, with reduction of their cellular size. Dopamine 174-182 glucose-6-phosphate isomerase Homo sapiens 77-109 31803912-1 2020 Our previous postmortem studies on neonates with neuropathological injury of perinatal hypoxia/ischemia (PHI) showed a dramatic reduction of tyrosine hydroxylase expression (dopamine synthesis enzyme) in substantia nigra (SN) neurons, with reduction of their cellular size. Enzyme Inhibitors 193-199 glucose-6-phosphate isomerase Homo sapiens 77-109 31814681-5 2019 Phosphoglucose isomerase, also known as glucose-6-phosphate isomerase (GPI), which ranked first among 27 candidate genes, was further investigated by a new analytical tool namely enviro-geno-pheno-state (E-GPS) analysis. PhenoFluor 179-196 glucose-6-phosphate isomerase Homo sapiens 0-24 32252059-9 2020 CONCLUSION: This result suggests that the PFKL rs2073436C>G and GPI rs7248411C>G are useful for predicting the clinical outcome of first-line paclitaxel-cisplatin chemotherapy in NSCLC. Paclitaxel 142-152 glucose-6-phosphate isomerase Homo sapiens 64-67 32252059-9 2020 CONCLUSION: This result suggests that the PFKL rs2073436C>G and GPI rs7248411C>G are useful for predicting the clinical outcome of first-line paclitaxel-cisplatin chemotherapy in NSCLC. Cisplatin 153-162 glucose-6-phosphate isomerase Homo sapiens 64-67 31905745-2 2019 Acting as a d-glucose mimic, 2-DG inhibits glycolysis due to formation and intracellular accumulation of 2-deoxy-d-glucose-6-phosphate (2-DG6P), inhibiting the function of hexokinase and glucose-6-phosphate isomerase, and inducing cell death. Deoxyglucose 29-33 glucose-6-phosphate isomerase Homo sapiens 187-216 31905745-2 2019 Acting as a d-glucose mimic, 2-DG inhibits glycolysis due to formation and intracellular accumulation of 2-deoxy-d-glucose-6-phosphate (2-DG6P), inhibiting the function of hexokinase and glucose-6-phosphate isomerase, and inducing cell death. 2-dg6p 136-142 glucose-6-phosphate isomerase Homo sapiens 187-216 31814681-5 2019 Phosphoglucose isomerase, also known as glucose-6-phosphate isomerase (GPI), which ranked first among 27 candidate genes, was further investigated by a new analytical tool namely enviro-geno-pheno-state (E-GPS) analysis. PhenoFluor 179-196 glucose-6-phosphate isomerase Homo sapiens 40-69 31814681-5 2019 Phosphoglucose isomerase, also known as glucose-6-phosphate isomerase (GPI), which ranked first among 27 candidate genes, was further investigated by a new analytical tool namely enviro-geno-pheno-state (E-GPS) analysis. PhenoFluor 179-196 glucose-6-phosphate isomerase Homo sapiens 71-74 31814681-9 2019 Down-regulation of GPI in tumor tissues correlated well with depressed glucose metabolism and fatty acid synthesis, as well as enhanced fatty acid oxidation and creatine metabolism, indicating that GPI represents a suitable marker for increased probability of EMT in GC cells. Fatty Acids 94-104 glucose-6-phosphate isomerase Homo sapiens 19-22 31814681-9 2019 Down-regulation of GPI in tumor tissues correlated well with depressed glucose metabolism and fatty acid synthesis, as well as enhanced fatty acid oxidation and creatine metabolism, indicating that GPI represents a suitable marker for increased probability of EMT in GC cells. Fatty Acids 136-146 glucose-6-phosphate isomerase Homo sapiens 19-22 31275687-0 2019 Psychiatric and Behavioral Complications of GPi DBS in an Adolescent with Myoclonus Dystonia. dibromsalan 48-51 glucose-6-phosphate isomerase Homo sapiens 44-47 31301422-4 2019 Typically, intracellular pH (pHi) is reduced and recovery is to some degree coupled to pHe recovery (coupled pH regulation). Phenylalanine 87-90 glucose-6-phosphate isomerase Homo sapiens 29-32 31301422-6 2019 At PCO2 values beyond that which fish can compensate pHe, some fish are able to fully protect pHi despite large sustained reductions in pHe (preferential pHi regulation) and can tolerate PCO2 > 45 Torr. Phenylalanine 53-56 glucose-6-phosphate isomerase Homo sapiens 94-97 31301422-7 2019 This review discusses pHe and pHi regulation during exposure to hypercarbia starting with modeling the capacity and theoretical limit to pHe compensation in 19 studies. Phenylalanine 137-140 glucose-6-phosphate isomerase Homo sapiens 30-33 31301422-8 2019 Next, we discuss how fish compensate severe acute hypercarbia exposures beyond the putative limit of pHe compensation using preferential pHi regulation which has recently been observed to be common among fish subjected to severe hypercarbia. Phenylalanine 101-104 glucose-6-phosphate isomerase Homo sapiens 137-140 31272044-5 2019 Further investigation revealed that TBBPA inhibited the pathways of glucolysis and pentose phosphate, and promoted glyoxylate bypass via regulating genes expressions of key enzymes (such as glucose-6-phosphate isomerase, pyruvate dehydrogenase, isocitrate lyase, etc. tetrabromobisphenol A 36-41 glucose-6-phosphate isomerase Homo sapiens 190-219 31394311-6 2019 Glucose-6-phosphate isomerase (GPI), a rate-limiting enzyme converting glucose-6-phosphate to fructose-6-phosphate, was found to be significantly decreased in asthenozoospermia by Western blotting and ELISA on an extended sample size. Glucose-6-Phosphate 71-90 glucose-6-phosphate isomerase Homo sapiens 0-29 31394311-6 2019 Glucose-6-phosphate isomerase (GPI), a rate-limiting enzyme converting glucose-6-phosphate to fructose-6-phosphate, was found to be significantly decreased in asthenozoospermia by Western blotting and ELISA on an extended sample size. Glucose-6-Phosphate 71-90 glucose-6-phosphate isomerase Homo sapiens 31-34 31394311-6 2019 Glucose-6-phosphate isomerase (GPI), a rate-limiting enzyme converting glucose-6-phosphate to fructose-6-phosphate, was found to be significantly decreased in asthenozoospermia by Western blotting and ELISA on an extended sample size. fructose-6-phosphate 94-114 glucose-6-phosphate isomerase Homo sapiens 0-29 31394311-6 2019 Glucose-6-phosphate isomerase (GPI), a rate-limiting enzyme converting glucose-6-phosphate to fructose-6-phosphate, was found to be significantly decreased in asthenozoospermia by Western blotting and ELISA on an extended sample size. fructose-6-phosphate 94-114 glucose-6-phosphate isomerase Homo sapiens 31-34 31394311-11 2019 The supplement of the product of GPI, fructose-6-phosphate, could significantly improve sperm motility. fructose-6-phosphate 38-58 glucose-6-phosphate isomerase Homo sapiens 33-36 31392597-7 2019 METHODS: Using a 9.4 T MRI scanner, CEST spectra were acquired sensitive to pHi using amine/amide concentration independent detection (AACID). Amines 86-91 glucose-6-phosphate isomerase Homo sapiens 76-79 31392597-7 2019 METHODS: Using a 9.4 T MRI scanner, CEST spectra were acquired sensitive to pHi using amine/amide concentration independent detection (AACID). Amides 92-97 glucose-6-phosphate isomerase Homo sapiens 76-79 31392597-10 2019 After injecting the drug combination with glucose the AACID value increased by 0.18 +- 0.03 corresponding to a 0.72 decrease in pHi. Glucose 42-49 glucose-6-phosphate isomerase Homo sapiens 128-131 31349224-1 2019 Stimulating cholinergic neurons causes urinary incontinence after DBS of the Gpi. dibromsalan 66-69 glucose-6-phosphate isomerase Homo sapiens 77-80 31295864-2 2019 The reversal of the pH gradient across the plasma membrane in cells that regulate intracellular pH (pHi) has potential to drive the selective uptake of weak acids at low extracellular pH (pHe). Phenylalanine 188-191 glucose-6-phosphate isomerase Homo sapiens 100-103 31947492-4 2019 A recent study has further introduced a metric called Glucose Profile Indicator (GPI) for CGM based diabetes management including a subset of the recommended CGM metrics. Glucose 54-61 glucose-6-phosphate isomerase Homo sapiens 81-84 31533238-9 2019 In a study of patients with recurrent thyroid cancer, expression levels of specific ribosomal machinery-namely PIGU (phosphatidylinositol glycan anchor biosynthesis class U), a subunit of the GPI (glycosylphosphatidylinositol transamidase complex-correlated with RAI avidity in radioiodine scanning, NIS levels, and biochemical response to RAI treatment. Glycosylphosphatidylinositols 117-144 glucose-6-phosphate isomerase Homo sapiens 192-195 31533238-9 2019 In a study of patients with recurrent thyroid cancer, expression levels of specific ribosomal machinery-namely PIGU (phosphatidylinositol glycan anchor biosynthesis class U), a subunit of the GPI (glycosylphosphatidylinositol transamidase complex-correlated with RAI avidity in radioiodine scanning, NIS levels, and biochemical response to RAI treatment. Iodine-131 278-289 glucose-6-phosphate isomerase Homo sapiens 192-195 31533238-9 2019 In a study of patients with recurrent thyroid cancer, expression levels of specific ribosomal machinery-namely PIGU (phosphatidylinositol glycan anchor biosynthesis class U), a subunit of the GPI (glycosylphosphatidylinositol transamidase complex-correlated with RAI avidity in radioiodine scanning, NIS levels, and biochemical response to RAI treatment. Nickel 300-303 glucose-6-phosphate isomerase Homo sapiens 192-195 31048086-10 2019 pGI reduced early mortality associated with CRGNB in colonized patients undergoing post-transplant cyclophosphamide-based haploidentical HCT. Cyclophosphamide 99-115 glucose-6-phosphate isomerase Homo sapiens 0-3 31104127-5 2019 Moreover, application of the CO2/HCO3--free solution did not change intracellular pH (pHi), and addition of an inhibitor of carbonic anhydrase (acetazolamide) sustained pHi increase induced by the NH4+ pulse, which transiently increased pHi in the absence of acetazolamide. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 29-32 glucose-6-phosphate isomerase Homo sapiens 169-172 31104127-5 2019 Moreover, application of the CO2/HCO3--free solution did not change intracellular pH (pHi), and addition of an inhibitor of carbonic anhydrase (acetazolamide) sustained pHi increase induced by the NH4+ pulse, which transiently increased pHi in the absence of acetazolamide. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 29-32 glucose-6-phosphate isomerase Homo sapiens 169-172 31104127-5 2019 Moreover, application of the CO2/HCO3--free solution did not change intracellular pH (pHi), and addition of an inhibitor of carbonic anhydrase (acetazolamide) sustained pHi increase induced by the NH4+ pulse, which transiently increased pHi in the absence of acetazolamide. Acetazolamide 144-157 glucose-6-phosphate isomerase Homo sapiens 169-172 31104127-5 2019 Moreover, application of the CO2/HCO3--free solution did not change intracellular pH (pHi), and addition of an inhibitor of carbonic anhydrase (acetazolamide) sustained pHi increase induced by the NH4+ pulse, which transiently increased pHi in the absence of acetazolamide. Acetazolamide 144-157 glucose-6-phosphate isomerase Homo sapiens 169-172 31104127-6 2019 These results indicate that the cHNEC produces a large amount of CO2, which maintains a constant pHi even under the CO2/HCO3--free condition. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 65-68 glucose-6-phosphate isomerase Homo sapiens 97-100 31104127-6 2019 These results indicate that the cHNEC produces a large amount of CO2, which maintains a constant pHi even under the CO2/HCO3--free condition. N2,N6-bis(4-(2-aminoethoxy)quinolin-2-yl)-4-((4-fluorobenzyl)oxy)pyridine-2,6-dicarboxamide 116-119 glucose-6-phosphate isomerase Homo sapiens 97-100 31104127-6 2019 These results indicate that the cHNEC produces a large amount of CO2, which maintains a constant pHi even under the CO2/HCO3--free condition. Bicarbonates 120-124 glucose-6-phosphate isomerase Homo sapiens 97-100 33405581-3 2019 Benefiting from the acid-triggered volume expansion of CaCO3, DSA/CC-DOX NPs can act like a "lysosomal bomb" that rapidly tears the lysosomal membrane with the release of acidic inclusions and the loaded DOX, and then the alkalized pHi in human liver tumor cells (HepG2) can be decreased from 7.61 to 7.09, thus promoting the intracellular accumulation of DOX nearly 3 times more than the free drug. Calcium Carbonate 55-60 glucose-6-phosphate isomerase Homo sapiens 232-235 33405581-3 2019 Benefiting from the acid-triggered volume expansion of CaCO3, DSA/CC-DOX NPs can act like a "lysosomal bomb" that rapidly tears the lysosomal membrane with the release of acidic inclusions and the loaded DOX, and then the alkalized pHi in human liver tumor cells (HepG2) can be decreased from 7.61 to 7.09, thus promoting the intracellular accumulation of DOX nearly 3 times more than the free drug. dsa 62-65 glucose-6-phosphate isomerase Homo sapiens 232-235 33405581-3 2019 Benefiting from the acid-triggered volume expansion of CaCO3, DSA/CC-DOX NPs can act like a "lysosomal bomb" that rapidly tears the lysosomal membrane with the release of acidic inclusions and the loaded DOX, and then the alkalized pHi in human liver tumor cells (HepG2) can be decreased from 7.61 to 7.09, thus promoting the intracellular accumulation of DOX nearly 3 times more than the free drug. Doxorubicin 69-72 glucose-6-phosphate isomerase Homo sapiens 232-235 31124647-4 2019 With ultraviolet photoelectron spectroscopy (UPS) and Kelvin probe, we evidence that even minute UV photon fluxes (500 times lower than that used in typical UPS experiments) are sufficient to induce SPV and shift the perovskite Phi and VBM by several 100 meV compared to dark. spv 199-202 glucose-6-phosphate isomerase Homo sapiens 228-231 31431834-8 2019 Gp78/AMFR expression and AMF uptake are more closely associated with DTC compared to benign thyroid lesions or ATC and with PTC-derived cancer stem-like cells. dtc 69-72 glucose-6-phosphate isomerase Homo sapiens 5-8 30590026-10 2019 Thus, LEV lowered the pHi of human neocortical pyramidal cells most likely by a weakening of the transmembrane HCO3(-)-mediated acid-extrusion. Levetiracetam 6-9 glucose-6-phosphate isomerase Homo sapiens 22-25 31133865-2 2019 Glucose-6-phosphate isomerase (GPI, EC 5.3.1.9) is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate, the second reaction step of glycolysis. Glucose-6-Phosphate 115-134 glucose-6-phosphate isomerase Homo sapiens 0-29 31133865-2 2019 Glucose-6-phosphate isomerase (GPI, EC 5.3.1.9) is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate, the second reaction step of glycolysis. Glucose-6-Phosphate 115-134 glucose-6-phosphate isomerase Homo sapiens 31-34 31133865-2 2019 Glucose-6-phosphate isomerase (GPI, EC 5.3.1.9) is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate, the second reaction step of glycolysis. fructose-6-phosphate 138-158 glucose-6-phosphate isomerase Homo sapiens 0-29 31133865-2 2019 Glucose-6-phosphate isomerase (GPI, EC 5.3.1.9) is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate, the second reaction step of glycolysis. fructose-6-phosphate 138-158 glucose-6-phosphate isomerase Homo sapiens 31-34 30590026-4 2019 Recovery-slope from intracellular acidification following an ammonium prepulse (APP) was used to measure the pHi-regulation. Ammonium Compounds 61-69 glucose-6-phosphate isomerase Homo sapiens 109-112 30590026-12 2019 Neurons with more acidic resting pHi-values showed a minimal alkalization upon LEV providing a mechanism for paradoxical proconvulsive LEV-effects rarely observed in epilepsy patients. Levetiracetam 79-82 glucose-6-phosphate isomerase Homo sapiens 33-36 30590026-5 2019 Among twenty pyramidal cells exposed to 50 muM LEV, the resting pHi (7.09 +- 0.14) was lowered in eight (40%) neurons, on average by 0.02 +- 0.011 pH-units. Levetiracetam 47-50 glucose-6-phosphate isomerase Homo sapiens 64-67 30590026-12 2019 Neurons with more acidic resting pHi-values showed a minimal alkalization upon LEV providing a mechanism for paradoxical proconvulsive LEV-effects rarely observed in epilepsy patients. Levetiracetam 135-138 glucose-6-phosphate isomerase Homo sapiens 33-36 30590026-7 2019 The LEV-induced pHi-shifts were positively correlated with the resting pHi (r = 0.6, p = 0.006, n = 20). Levetiracetam 4-7 glucose-6-phosphate isomerase Homo sapiens 16-19 30654594-4 2019 %), the time taken by the alloy to achieve the peak hardness value gradually increases aging at 120 C. When the Zn/Mg ratio is in the range from 2.27% to 2.62%, the precipitate phase of the alloy after peak-aged is mainly dominated by smaller disc-like eta" phase and GP I (Guinier Preston) zones, the grain boundary precipitates are slender and continuous and the PFZ (precipitate free zones) is narrow. Zinc 113-115 glucose-6-phosphate isomerase Homo sapiens 269-273 30590026-7 2019 The LEV-induced pHi-shifts were positively correlated with the resting pHi (r = 0.6, p = 0.006, n = 20). Levetiracetam 4-7 glucose-6-phosphate isomerase Homo sapiens 71-74 30971931-4 2019 Transmembrane cystein mutation of Na+- independent Cl-/HCO3 - exchanger (anion exchanger, AE) affects pHi. Cysteine 14-21 glucose-6-phosphate isomerase Homo sapiens 102-105 30971931-8 2019 Concurrently, NH4Cl pre-perfusion showed that SO2 significantly activated AE, whereas the AE inhibitor 4,4"-diisothiocyanatostilbene- 2,20-disulfonic acid (DIDS) significantly attenuated the effect of SO2 on pHi in VSMCs. 4,4"-diisothiocyanatostilbene- 2,20-disulfonic acid 103-154 glucose-6-phosphate isomerase Homo sapiens 208-211 30971931-8 2019 Concurrently, NH4Cl pre-perfusion showed that SO2 significantly activated AE, whereas the AE inhibitor 4,4"-diisothiocyanatostilbene- 2,20-disulfonic acid (DIDS) significantly attenuated the effect of SO2 on pHi in VSMCs. 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid 156-160 glucose-6-phosphate isomerase Homo sapiens 208-211 30971931-9 2019 While 200 muM SO2 sulphenylated AE2, while dithiothreitol (DTT) blocked the sulphenylation of AE2 and subsequent AE activation by SO2, thereby restoring the pHi in VSMCs. Dithiothreitol 43-57 glucose-6-phosphate isomerase Homo sapiens 157-160 30971931-9 2019 While 200 muM SO2 sulphenylated AE2, while dithiothreitol (DTT) blocked the sulphenylation of AE2 and subsequent AE activation by SO2, thereby restoring the pHi in VSMCs. Dithiothreitol 59-62 glucose-6-phosphate isomerase Homo sapiens 157-160 30971931-9 2019 While 200 muM SO2 sulphenylated AE2, while dithiothreitol (DTT) blocked the sulphenylation of AE2 and subsequent AE activation by SO2, thereby restoring the pHi in VSMCs. Sulfur Dioxide 130-133 glucose-6-phosphate isomerase Homo sapiens 157-160 30697740-4 2019 In this study, we verified that after transient exposure to NH4 Cl, the pHi values could rapidly recover from acidification via Na+ -H+ exchanger (NHE), Na+ -HCO3 - cotransporter (NBC), and carbonic anhydrase in human vaginal epithelial cell line VK2/E6E7. Ammonium Chloride 60-66 glucose-6-phosphate isomerase Homo sapiens 72-75 30740291-3 2019 The L4-based RuCl3 3H2O system corresponded to the best conversion of PhI (96 %) along with 99 % selectivity to the target product of methyl benzoate as well as the good generality to alkoxycarbonylation of different aryl halides (ArX, X=I and Br) with alcohols MeOH, EtOH, i-PrOH and n-BuOH. Methanol 264-268 glucose-6-phosphate isomerase Homo sapiens 72-75 30740291-3 2019 The L4-based RuCl3 3H2O system corresponded to the best conversion of PhI (96 %) along with 99 % selectivity to the target product of methyl benzoate as well as the good generality to alkoxycarbonylation of different aryl halides (ArX, X=I and Br) with alcohols MeOH, EtOH, i-PrOH and n-BuOH. Ethanol 270-274 glucose-6-phosphate isomerase Homo sapiens 72-75 30740291-3 2019 The L4-based RuCl3 3H2O system corresponded to the best conversion of PhI (96 %) along with 99 % selectivity to the target product of methyl benzoate as well as the good generality to alkoxycarbonylation of different aryl halides (ArX, X=I and Br) with alcohols MeOH, EtOH, i-PrOH and n-BuOH. 2-Propanol 276-282 glucose-6-phosphate isomerase Homo sapiens 72-75 30740291-3 2019 The L4-based RuCl3 3H2O system corresponded to the best conversion of PhI (96 %) along with 99 % selectivity to the target product of methyl benzoate as well as the good generality to alkoxycarbonylation of different aryl halides (ArX, X=I and Br) with alcohols MeOH, EtOH, i-PrOH and n-BuOH. Butanols 287-293 glucose-6-phosphate isomerase Homo sapiens 72-75 30589268-2 2019 The use of the benzimidazole ring as the internal nucleophile and the use of phenyliodosophthalate (PhI(Phth)), a new metal-free and low toxic hypervalent iodine reagent, are the most remarkable novelties of this synthetic strategy. phenyliodosophthalate 77-98 glucose-6-phosphate isomerase Homo sapiens 100-109 30589268-2 2019 The use of the benzimidazole ring as the internal nucleophile and the use of phenyliodosophthalate (PhI(Phth)), a new metal-free and low toxic hypervalent iodine reagent, are the most remarkable novelties of this synthetic strategy. Metals 118-123 glucose-6-phosphate isomerase Homo sapiens 100-109 30589268-2 2019 The use of the benzimidazole ring as the internal nucleophile and the use of phenyliodosophthalate (PhI(Phth)), a new metal-free and low toxic hypervalent iodine reagent, are the most remarkable novelties of this synthetic strategy. Iodine 155-161 glucose-6-phosphate isomerase Homo sapiens 100-109 30589268-3 2019 With this approach, we have demonstrated the usefulness of the fragmentation of anomeric alkoxyl radicals promoted by the PhI(Phth)/I2 system for the preparation of new compounds with potential interest for both medicinal and synthetic chemists. alkoxyl radical 89-105 glucose-6-phosphate isomerase Homo sapiens 122-131 30740291-3 2019 The L4-based RuCl3 3H2O system corresponded to the best conversion of PhI (96 %) along with 99 % selectivity to the target product of methyl benzoate as well as the good generality to alkoxycarbonylation of different aryl halides (ArX, X=I and Br) with alcohols MeOH, EtOH, i-PrOH and n-BuOH. rucl3 3h2o 13-25 glucose-6-phosphate isomerase Homo sapiens 72-75 30740291-3 2019 The L4-based RuCl3 3H2O system corresponded to the best conversion of PhI (96 %) along with 99 % selectivity to the target product of methyl benzoate as well as the good generality to alkoxycarbonylation of different aryl halides (ArX, X=I and Br) with alcohols MeOH, EtOH, i-PrOH and n-BuOH. methyl benzoate 136-151 glucose-6-phosphate isomerase Homo sapiens 72-75 30740291-3 2019 The L4-based RuCl3 3H2O system corresponded to the best conversion of PhI (96 %) along with 99 % selectivity to the target product of methyl benzoate as well as the good generality to alkoxycarbonylation of different aryl halides (ArX, X=I and Br) with alcohols MeOH, EtOH, i-PrOH and n-BuOH. aryl halides 219-231 glucose-6-phosphate isomerase Homo sapiens 72-75 30740291-3 2019 The L4-based RuCl3 3H2O system corresponded to the best conversion of PhI (96 %) along with 99 % selectivity to the target product of methyl benzoate as well as the good generality to alkoxycarbonylation of different aryl halides (ArX, X=I and Br) with alcohols MeOH, EtOH, i-PrOH and n-BuOH. Alcohols 255-263 glucose-6-phosphate isomerase Homo sapiens 72-75 30628770-4 2019 Prior addition of substrate to the reaction of PhI NTs with the iron(II) complex attenuates the CT absorbance of the equilibrium solution. ammonium ferrous sulfate 64-72 glucose-6-phosphate isomerase Homo sapiens 47-50 30664618-7 2019 PGAP1 modifies GPI-anchors through inositol deacylation, allowing it to be recognized by Tmp21. Inositol 35-43 glucose-6-phosphate isomerase Homo sapiens 15-18 30071192-1 2019 The mammalian Na+/H+ exchanger isoform 1 (NHE1) is an integral membrane protein that regulates intracellular pH (pHi) by removing a single intracellular proton in exchange for one extracellular sodium ion. Sodium 194-200 glucose-6-phosphate isomerase Homo sapiens 113-116 30466785-5 2019 Mechanistically, VPA suppresses aerobic glycolysis via reducing the levels of E2F transcription factor 1 (E2F1), resulting in repressed expression of glycolytic genes glucose-6-phosphate isomerase (GPI) and phosphoglycerate pinase 1 (PGK1). Valproic Acid 17-20 glucose-6-phosphate isomerase Homo sapiens 167-196 30466785-5 2019 Mechanistically, VPA suppresses aerobic glycolysis via reducing the levels of E2F transcription factor 1 (E2F1), resulting in repressed expression of glycolytic genes glucose-6-phosphate isomerase (GPI) and phosphoglycerate pinase 1 (PGK1). Valproic Acid 17-20 glucose-6-phosphate isomerase Homo sapiens 198-201