PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 34536182-12 2022 Expression of eNOS and UCP2 mRNA and NO production were decreased after DOX exposure, and PBMT preconditioning before the DOX challenge reversed these changes. Doxorubicin 122-125 uncoupling protein 2 Homo sapiens 23-27 33763373-3 2021 Neoplastic cells, especially those with high proliferative potential such as GSCs, have been shown to upregulate UCP2 as a cytoprotective mechanism in response to chronic increased reactive oxygen species (ROS) exposure. Reactive Oxygen Species 181-204 uncoupling protein 2 Homo sapiens 113-117 33763373-3 2021 Neoplastic cells, especially those with high proliferative potential such as GSCs, have been shown to upregulate UCP2 as a cytoprotective mechanism in response to chronic increased reactive oxygen species (ROS) exposure. Reactive Oxygen Species 206-209 uncoupling protein 2 Homo sapiens 113-117 32858880-0 2020 Increased Risk of High Body Fat and Altered Lipid Metabolism Associated to Suboptimal Consumption of Vitamin A Is Modulated by Genetic Variants rs5888 (SCARB1), rs1800629 (UCP1) and rs659366 (UCP2). Vitamin A 101-110 uncoupling protein 2 Homo sapiens 192-196 26123492-12 2015 Interestingly, transcript levels of the carriers for aspartate/glutamate AGC2, malate DIC and malate/oxaloacetate/aspartate UCP2 were increased by high glucose, a profile suggesting important mitochondrial anaplerotic/cataplerotic activities and NADPH-generating shuttles. Oxaloacetic Acid 101-113 uncoupling protein 2 Homo sapiens 124-128 26123492-12 2015 Interestingly, transcript levels of the carriers for aspartate/glutamate AGC2, malate DIC and malate/oxaloacetate/aspartate UCP2 were increased by high glucose, a profile suggesting important mitochondrial anaplerotic/cataplerotic activities and NADPH-generating shuttles. Aspartic Acid 114-123 uncoupling protein 2 Homo sapiens 124-128 26123492-12 2015 Interestingly, transcript levels of the carriers for aspartate/glutamate AGC2, malate DIC and malate/oxaloacetate/aspartate UCP2 were increased by high glucose, a profile suggesting important mitochondrial anaplerotic/cataplerotic activities and NADPH-generating shuttles. Glucose 152-159 uncoupling protein 2 Homo sapiens 124-128 26123492-12 2015 Interestingly, transcript levels of the carriers for aspartate/glutamate AGC2, malate DIC and malate/oxaloacetate/aspartate UCP2 were increased by high glucose, a profile suggesting important mitochondrial anaplerotic/cataplerotic activities and NADPH-generating shuttles. NADP 246-251 uncoupling protein 2 Homo sapiens 124-128 34625529-0 2021 An interplay between UCP2 and ROS protects cells from high-salt-induced injury through autophagy stimulation. Salts 59-63 uncoupling protein 2 Homo sapiens 21-25 34146128-10 2021 Moreover, UCP-2 inhibitor Genipin significantly enhanced the proliferation suppression effects of trastuzumab and markedly promoted apoptosis. genipin 26-33 uncoupling protein 2 Homo sapiens 10-15 34719264-1 2021 Background Isolated loss-of-function single nucleotide polymorphisms (SNPs) for SIRT3 (a mitochondrial deacetylase) and UCP2 (an atypical uncoupling protein enabling mitochondrial calcium entry) have been associated with both pulmonary arterial hypertension (PAH) and insulin resistance, but their collective role in animal models and patients is unknown. Calcium 180-187 uncoupling protein 2 Homo sapiens 120-124 34710532-0 2021 Neuroprotection of retinal cells by Caffeic Acid Phenylethyl Ester(CAPE) is mediated by mitochondrial uncoupling protein UCP2. caffeic acid phenethyl ester 36-66 uncoupling protein 2 Homo sapiens 121-125 34710532-0 2021 Neuroprotection of retinal cells by Caffeic Acid Phenylethyl Ester(CAPE) is mediated by mitochondrial uncoupling protein UCP2. caffeic acid phenethyl ester 67-71 uncoupling protein 2 Homo sapiens 121-125 34710532-5 2021 The major effect of CAPE was mediated by the mitochondrial uncoupling protein UCP2 since both pharmacological inhibition of UCP2 and siRNA-induced knockdown removed the ability of CAPE to block ROS production. caffeic acid phenethyl ester 20-24 uncoupling protein 2 Homo sapiens 78-82 34710532-5 2021 The major effect of CAPE was mediated by the mitochondrial uncoupling protein UCP2 since both pharmacological inhibition of UCP2 and siRNA-induced knockdown removed the ability of CAPE to block ROS production. caffeic acid phenethyl ester 20-24 uncoupling protein 2 Homo sapiens 124-128 34710532-5 2021 The major effect of CAPE was mediated by the mitochondrial uncoupling protein UCP2 since both pharmacological inhibition of UCP2 and siRNA-induced knockdown removed the ability of CAPE to block ROS production. caffeic acid phenethyl ester 180-184 uncoupling protein 2 Homo sapiens 78-82 34710532-5 2021 The major effect of CAPE was mediated by the mitochondrial uncoupling protein UCP2 since both pharmacological inhibition of UCP2 and siRNA-induced knockdown removed the ability of CAPE to block ROS production. caffeic acid phenethyl ester 180-184 uncoupling protein 2 Homo sapiens 124-128 34710532-5 2021 The major effect of CAPE was mediated by the mitochondrial uncoupling protein UCP2 since both pharmacological inhibition of UCP2 and siRNA-induced knockdown removed the ability of CAPE to block ROS production. Reactive Oxygen Species 194-197 uncoupling protein 2 Homo sapiens 78-82 34710532-6 2021 Based on common structural features, CAPE may be acting as a mimetic of the natural UCP2 homeostatic regulator 4-hydroxy-2-nonenal. caffeic acid phenethyl ester 37-41 uncoupling protein 2 Homo sapiens 84-88 34710532-6 2021 Based on common structural features, CAPE may be acting as a mimetic of the natural UCP2 homeostatic regulator 4-hydroxy-2-nonenal. 4-hydroxy-2-nonenal 111-130 uncoupling protein 2 Homo sapiens 84-88 34748771-3 2022 The aim of the current study was to investigate whether mild sub-lethal stress induced by low concentrations of nitric oxide and hydrogen peroxide has a protective effect on quality parameters of post-thaw bull semen through modulations of mitochondrial uncoupling protein 2 (UCP2) expression. Nitric Oxide 112-124 uncoupling protein 2 Homo sapiens 240-274 34748771-3 2022 The aim of the current study was to investigate whether mild sub-lethal stress induced by low concentrations of nitric oxide and hydrogen peroxide has a protective effect on quality parameters of post-thaw bull semen through modulations of mitochondrial uncoupling protein 2 (UCP2) expression. Nitric Oxide 112-124 uncoupling protein 2 Homo sapiens 276-280 34748771-3 2022 The aim of the current study was to investigate whether mild sub-lethal stress induced by low concentrations of nitric oxide and hydrogen peroxide has a protective effect on quality parameters of post-thaw bull semen through modulations of mitochondrial uncoupling protein 2 (UCP2) expression. Hydrogen Peroxide 129-146 uncoupling protein 2 Homo sapiens 240-274 34748771-3 2022 The aim of the current study was to investigate whether mild sub-lethal stress induced by low concentrations of nitric oxide and hydrogen peroxide has a protective effect on quality parameters of post-thaw bull semen through modulations of mitochondrial uncoupling protein 2 (UCP2) expression. Hydrogen Peroxide 129-146 uncoupling protein 2 Homo sapiens 276-280 34748771-8 2022 In addition, the expression level of UCP2 was higher in the NO-1 and H2O2-10 compared to the other groups (P < 0.05). Hydrogen Peroxide 69-73 uncoupling protein 2 Homo sapiens 37-41 34419618-5 2021 Activating TRPA1 with cinnamaldehyde prevented downregulation of eNOS, Nrf2, and UCP2, inhibited superoxide production and apoptosis, and preserved nitric oxide bioavailability in senescent HUVECs. cinnamaldehyde 22-36 uncoupling protein 2 Homo sapiens 81-85 34419618-7 2021 Dietary administration of cinnamaldehyde for 12 months prevented mitochondrial dysfunction, improved endothelium-dependent relaxation, and increased expression of eNOS, Nrf2, and UCP2 in aged aortas. cinnamaldehyde 26-40 uncoupling protein 2 Homo sapiens 179-183 34174105-0 2021 Klotho inhibits H2 O2 -induced oxidative stress and apoptosis in periodontal ligament stem cells by regulating UCP2 expression. Hydrogen Peroxide 16-21 uncoupling protein 2 Homo sapiens 111-115 34174105-11 2021 Besides, klotho upregulated the production of UCP2 in H2 O2 -treated PDLSCs. Hydrogen Peroxide 54-59 uncoupling protein 2 Homo sapiens 46-50 34174105-14 2021 In conclusion, this study showed that klotho inhibits H2 O2 -induced oxidative stress and apoptosis in PDLSCs by regulating UCP2 expression. Hydrogen Peroxide 54-59 uncoupling protein 2 Homo sapiens 124-128 34536182-12 2022 Expression of eNOS and UCP2 mRNA and NO production were decreased after DOX exposure, and PBMT preconditioning before the DOX challenge reversed these changes. Doxorubicin 72-75 uncoupling protein 2 Homo sapiens 23-27 34458651-0 2021 The Effect of omega3 Fatty Acids Supplementation on Levels of PPARgamma and UCP2 Genes Expression, Serum Level of UCP2 Protein, Metabolic Status, and Appetite in Elite male Athletes: Protocol for a Randomized Control Trial. Fatty Acids, Omega-3 14-32 uncoupling protein 2 Homo sapiens 76-80 34458651-21 2021 Highlights: Omega3 fatty acids as a ligand of metabolic-related genes, have a role in energy expenditure.Omega3 supplements effect on PPARgamma and UCP2 mRNA expression as regulators of energy metabolismOmega3 supplements increased REE.Omega-3 supplementation could change the changes in body composition.For athletes, omega-3 simultaneously decreased fat mass and increased fat-mass.HDL-C increased after short-term supplementation with omega-3.Increased intake of omega-3, caused increased intake of energy and protein. Fatty Acids, Omega-3 12-30 uncoupling protein 2 Homo sapiens 148-152 34458651-3 2021 The objective of the present study is to determine the effects of omega3 fatty acids on the gene expression of PPARgamma and UCP2, levels of blood lipid profile, fat mass, and fat-free mass, and appetite. Fatty Acids, Omega-3 66-84 uncoupling protein 2 Homo sapiens 125-129 34458651-21 2021 Highlights: Omega3 fatty acids as a ligand of metabolic-related genes, have a role in energy expenditure.Omega3 supplements effect on PPARgamma and UCP2 mRNA expression as regulators of energy metabolismOmega3 supplements increased REE.Omega-3 supplementation could change the changes in body composition.For athletes, omega-3 simultaneously decreased fat mass and increased fat-mass.HDL-C increased after short-term supplementation with omega-3.Increased intake of omega-3, caused increased intake of energy and protein. Fatty Acids, Omega-3 105-111 uncoupling protein 2 Homo sapiens 148-152 34458651-21 2021 Highlights: Omega3 fatty acids as a ligand of metabolic-related genes, have a role in energy expenditure.Omega3 supplements effect on PPARgamma and UCP2 mRNA expression as regulators of energy metabolismOmega3 supplements increased REE.Omega-3 supplementation could change the changes in body composition.For athletes, omega-3 simultaneously decreased fat mass and increased fat-mass.HDL-C increased after short-term supplementation with omega-3.Increased intake of omega-3, caused increased intake of energy and protein. Fatty Acids, Omega-3 319-326 uncoupling protein 2 Homo sapiens 148-152 34458651-21 2021 Highlights: Omega3 fatty acids as a ligand of metabolic-related genes, have a role in energy expenditure.Omega3 supplements effect on PPARgamma and UCP2 mRNA expression as regulators of energy metabolismOmega3 supplements increased REE.Omega-3 supplementation could change the changes in body composition.For athletes, omega-3 simultaneously decreased fat mass and increased fat-mass.HDL-C increased after short-term supplementation with omega-3.Increased intake of omega-3, caused increased intake of energy and protein. Fatty Acids, Omega-3 438-445 uncoupling protein 2 Homo sapiens 148-152 34458651-18 2021 This study could result in the effects of omega-3 fatty acids on PPARgamma, and UCP2 expressions, blood lipid profiles and body composition. Fatty Acids, Omega-3 42-61 uncoupling protein 2 Homo sapiens 80-84 34289004-6 2021 The T/T genotype of the UCP2 gene was associated with higher levels of glycated hemoglobin, pre- and postprandial glycemia and lipid oxidation rate, lower carbohydrate oxidation, and lower serum vitamin C levels. Carbohydrates 155-167 uncoupling protein 2 Homo sapiens 24-28 34439417-7 2021 The cellular components mediating the downregulation of ROS included extracellular signal-regulated kinase 1/2 (ERK1/2), nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and uncoupling protein 2 (UCP2). Reactive Oxygen Species 56-59 uncoupling protein 2 Homo sapiens 202-222 34439417-7 2021 The cellular components mediating the downregulation of ROS included extracellular signal-regulated kinase 1/2 (ERK1/2), nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and uncoupling protein 2 (UCP2). Reactive Oxygen Species 56-59 uncoupling protein 2 Homo sapiens 224-228 34289004-6 2021 The T/T genotype of the UCP2 gene was associated with higher levels of glycated hemoglobin, pre- and postprandial glycemia and lipid oxidation rate, lower carbohydrate oxidation, and lower serum vitamin C levels. Ascorbic Acid 195-204 uncoupling protein 2 Homo sapiens 24-28 35462195-10 2022 In addition, DBDPE decreased the UCP2 level and ATP synthesis in mitochondria both under in vitro and in vivo conditions, consequently leading to apoptosis via the Cytochrome C/Caspase-9/Caspase-3 pathway. decabromodiphenyl ethane 13-18 uncoupling protein 2 Homo sapiens 33-37 35462195-13 2022 In addition, the DBDPE-treated altered levels of UCP2, ATP, and apoptosis were also found to be significantly reversed by 5-aza in AC16 cells. decabromodiphenyl ethane 17-22 uncoupling protein 2 Homo sapiens 49-53 35462195-13 2022 In addition, the DBDPE-treated altered levels of UCP2, ATP, and apoptosis were also found to be significantly reversed by 5-aza in AC16 cells. Azacitidine 122-127 uncoupling protein 2 Homo sapiens 49-53 33530558-2 2021 In this work, we use a combination of homology modelling and subsequent microsecond molecular dynamics simulations of UCP2 in the DOPC phospholipid bilayer, starting from the structure of the mitochondrial ATP/ADP carrier (ANT) as a template. Adenosine Triphosphate 206-209 uncoupling protein 2 Homo sapiens 118-122 35629388-1 2022 The mitochondrial uncoupling protein 2 (UCP2) acts as an anion transporter and as an antioxidant factor able to reduce the reactive oxygen species level. oxygen species 132-146 uncoupling protein 2 Homo sapiens 4-38 35629388-1 2022 The mitochondrial uncoupling protein 2 (UCP2) acts as an anion transporter and as an antioxidant factor able to reduce the reactive oxygen species level. oxygen species 132-146 uncoupling protein 2 Homo sapiens 40-44 35044239-7 2022 CRITICAL ISSUES: Potential functions of UCP2 and UCP3 in striated muscles include a role in protection against mitochondria-dependent oxidative stress, as transporter for pyruvate, fatty acids and protons into and out of the mitochondria, and in metabolic sensing. Fatty Acids 181-192 uncoupling protein 2 Homo sapiens 40-44 35091238-8 2022 Moreover, UCP2, 4, and 5 are ubiquitously present in all brain regions that negatively regulate ROS production and inflammation, leading to the prevention of neuronal cell death. Reactive Oxygen Species 96-99 uncoupling protein 2 Homo sapiens 10-14 35091061-7 2022 Live-cell imaging revealed that T3-induced enhancement of mitochondrial Ca2+ uptake depends on the mitochondrial Ca2+ uniporter (MCU), UCP2, and PRMT1 that are essential for increased mitochondrial ATP ((ATP)mito) production after T3 treatment. Triiodothyronine 32-34 uncoupling protein 2 Homo sapiens 135-139 35091061-7 2022 Live-cell imaging revealed that T3-induced enhancement of mitochondrial Ca2+ uptake depends on the mitochondrial Ca2+ uniporter (MCU), UCP2, and PRMT1 that are essential for increased mitochondrial ATP ((ATP)mito) production after T3 treatment. Adenosine Triphosphate 198-201 uncoupling protein 2 Homo sapiens 135-139 35091061-7 2022 Live-cell imaging revealed that T3-induced enhancement of mitochondrial Ca2+ uptake depends on the mitochondrial Ca2+ uniporter (MCU), UCP2, and PRMT1 that are essential for increased mitochondrial ATP ((ATP)mito) production after T3 treatment. Adenosine Triphosphate 204-207 uncoupling protein 2 Homo sapiens 135-139 35091061-7 2022 Live-cell imaging revealed that T3-induced enhancement of mitochondrial Ca2+ uptake depends on the mitochondrial Ca2+ uniporter (MCU), UCP2, and PRMT1 that are essential for increased mitochondrial ATP ((ATP)mito) production after T3 treatment. Triiodothyronine 231-233 uncoupling protein 2 Homo sapiens 135-139 35091061-10 2022 Based on these results, we assume that thyroid hormones adjust (Ca2+)mito homeostasis by modulating the UCP2- and PRMT1-balanced (Ca2+)mito uptake via MCU in case of physiological ROS levels to convey their impact on mitochondrial ATP and ROS production. Reactive Oxygen Species 180-183 uncoupling protein 2 Homo sapiens 104-108 35091061-10 2022 Based on these results, we assume that thyroid hormones adjust (Ca2+)mito homeostasis by modulating the UCP2- and PRMT1-balanced (Ca2+)mito uptake via MCU in case of physiological ROS levels to convey their impact on mitochondrial ATP and ROS production. Adenosine Triphosphate 231-234 uncoupling protein 2 Homo sapiens 104-108 35091061-10 2022 Based on these results, we assume that thyroid hormones adjust (Ca2+)mito homeostasis by modulating the UCP2- and PRMT1-balanced (Ca2+)mito uptake via MCU in case of physiological ROS levels to convey their impact on mitochondrial ATP and ROS production. Reactive Oxygen Species 239-242 uncoupling protein 2 Homo sapiens 104-108 35204205-1 2022 Mitochondrial uncoupling proteins (UCP) 1-3 fulfill many physiological functions, ranging from non-shivering thermogenesis (UCP1) to glucose-stimulated insulin release (GSIS) and satiety signaling (UCP2) and muscle fuel metabolism (UCP3). Glucose 133-140 uncoupling protein 2 Homo sapiens 198-202 35204205-13 2022 However, some studies also generated evidence that UCP2/3 may mitigate oxidative stress by transporting Ca2+ into the matrix, exporting lipid hydroperoxides, or by transporting C-4 metabolites. Lipid Peroxides 136-156 uncoupling protein 2 Homo sapiens 51-57 35204205-13 2022 However, some studies also generated evidence that UCP2/3 may mitigate oxidative stress by transporting Ca2+ into the matrix, exporting lipid hydroperoxides, or by transporting C-4 metabolites. imciromab pentetate 177-180 uncoupling protein 2 Homo sapiens 51-57 35129048-12 2022 In addition, siRNA-mediated UCP2 knockdown further aggravated mitochondrial fragmentation and DeltaPsim depolarization and increased mitochondrial ROS production and cell apoptosis in HS-induced HUVECs, which were abolished by Drp1 inhibition. ros 147-150 uncoupling protein 2 Homo sapiens 28-32 33957975-1 2021 OBJECTIVE: Uncoupling protein 2 (UCP2) plays a crucial role in energy homeostasis via insulin secretion regulation, free fatty acid concentrations, and lipid metabolism. Fatty Acids, Nonesterified 116-131 uncoupling protein 2 Homo sapiens 11-31 33957975-1 2021 OBJECTIVE: Uncoupling protein 2 (UCP2) plays a crucial role in energy homeostasis via insulin secretion regulation, free fatty acid concentrations, and lipid metabolism. Fatty Acids, Nonesterified 116-131 uncoupling protein 2 Homo sapiens 33-37 33859525-0 2021 Effect of 5-azacytidine (5-aza) on UCP2 expression in human liver and colon cancer cells. Azacitidine 10-23 uncoupling protein 2 Homo sapiens 35-39 33859525-0 2021 Effect of 5-azacytidine (5-aza) on UCP2 expression in human liver and colon cancer cells. Azacitidine 10-15 uncoupling protein 2 Homo sapiens 35-39 33859525-9 2021 Treatment with 5-aza increased UCP2 expression in Hep3B and HT-29 cells; however, the expression in HepG2 cells was unchanged. Azacitidine 15-20 uncoupling protein 2 Homo sapiens 31-35 33598768-3 2021 Subcutaneous neck adipose tissue from cold-acclimated or triiodothyronine (T3)-treated chickens exhibited increases in the expression of avian uncoupling protein (avUCP, an ortholog of mammalian UCP2 and UCP3) gene and some known mammalian beige adipocyte-specific markers. Triiodothyronine 57-73 uncoupling protein 2 Homo sapiens 195-199 33598768-3 2021 Subcutaneous neck adipose tissue from cold-acclimated or triiodothyronine (T3)-treated chickens exhibited increases in the expression of avian uncoupling protein (avUCP, an ortholog of mammalian UCP2 and UCP3) gene and some known mammalian beige adipocyte-specific markers. Triiodothyronine 75-77 uncoupling protein 2 Homo sapiens 195-199 35044239-7 2022 CRITICAL ISSUES: Potential functions of UCP2 and UCP3 in striated muscles include a role in protection against mitochondria-dependent oxidative stress, as transporter for pyruvate, fatty acids and protons into and out of the mitochondria, and in metabolic sensing. Pyruvic Acid 171-179 uncoupling protein 2 Homo sapiens 40-44 35409033-0 2022 Protective Effects of Mitochondrial Uncoupling Protein 2 against Aristolochic Acid I-Induced Toxicity in HK-2 Cells. aristolochic acid I 65-84 uncoupling protein 2 Homo sapiens 22-56 35409033-2 2022 This paper was designed to assess whether mitochondrial Uncoupling Protein 2 (UCP2), which plays an antioxidative and antiapoptotic role, could protect human renal proximal tubular epithelial (HK-2) cells from toxicity induced by AA I. Iodine 233-234 uncoupling protein 2 Homo sapiens 42-76 35409033-2 2022 This paper was designed to assess whether mitochondrial Uncoupling Protein 2 (UCP2), which plays an antioxidative and antiapoptotic role, could protect human renal proximal tubular epithelial (HK-2) cells from toxicity induced by AA I. Iodine 233-234 uncoupling protein 2 Homo sapiens 78-82 35409033-3 2022 In this study, HK-2 cells were treated with different concentrations of AA I with or without UCP2 inhibitor (genipin). genipin 109-116 uncoupling protein 2 Homo sapiens 93-97 33964966-0 2021 The effect of omega3 fatty acid supplementation on PPARgamma and UCP2 expressions, resting energy expenditure, and appetite in athletes. Fatty Acids, Omega-3 14-31 uncoupling protein 2 Homo sapiens 65-69 33879866-3 2021 This was followed by a transient rise in microglial production of reactive oxygen species (ROS) and a concurrent increase in expression of uncoupling protein 2 (Ucp2), a regulator of mitochondrial ROS generation. Reactive Oxygen Species 197-200 uncoupling protein 2 Homo sapiens 139-159 33879866-3 2021 This was followed by a transient rise in microglial production of reactive oxygen species (ROS) and a concurrent increase in expression of uncoupling protein 2 (Ucp2), a regulator of mitochondrial ROS generation. Reactive Oxygen Species 197-200 uncoupling protein 2 Homo sapiens 161-165 33879866-4 2021 Conditional ablation of Ucp2 from microglia hindered phasic elimination of spine synapses with consequent accumulations of ROS and lysosome-lipid droplet complexes, which resulted in hippocampal neuronal circuit dysfunctions assessed by electrophysiology, and altered anxiety-like behavior. Reactive Oxygen Species 123-126 uncoupling protein 2 Homo sapiens 24-28 33530558-2 2021 In this work, we use a combination of homology modelling and subsequent microsecond molecular dynamics simulations of UCP2 in the DOPC phospholipid bilayer, starting from the structure of the mitochondrial ATP/ADP carrier (ANT) as a template. Adenosine Diphosphate 210-213 uncoupling protein 2 Homo sapiens 118-122 33530558-4 2021 We also show that ATP binding in the UCP2 cavity is tight in the homology modelled structure of UCP2 in agreement with experimental observations. Adenosine Triphosphate 18-21 uncoupling protein 2 Homo sapiens 37-41 33530558-4 2021 We also show that ATP binding in the UCP2 cavity is tight in the homology modelled structure of UCP2 in agreement with experimental observations. Adenosine Triphosphate 18-21 uncoupling protein 2 Homo sapiens 96-100 33530558-5 2021 Finally, we corroborate our results with conductance measurements in model membranes, which further suggest that the UCP2 structure modeled from ANT protein possesses additional key functional elements, such as a fatty acid-binding site at the R60 region of the protein, directly related to the proton transport mechanism across inner mitochondrial membranes. Fatty Acids 213-223 uncoupling protein 2 Homo sapiens 117-121 33532034-0 2021 Corrigendum to "Inhibition of Uncoupling Protein 2 Enhances the Radiosensitivity of Cervical Cancer Cells by Promoting the Production of Reactive Oxygen Species". Oxygen 146-152 uncoupling protein 2 Homo sapiens 30-50 33463495-0 2020 [Uncoupling protein 2 overexpression alleviates sepsis-induced myocardial injury via inhibiting reactive oxygen species production and inflammation]. Reactive Oxygen Species 96-119 uncoupling protein 2 Homo sapiens 1-21 33162463-2 2020 Uncoupling protein 2 (UCP2) is involved in the disappearance of reactive oxygen species, suggesting the defensive role of UCP2 against oxidative stress. Reactive Oxygen Species 64-87 uncoupling protein 2 Homo sapiens 0-20 33069910-2 2020 This review aims to revisit and shed light on the fundamental molecular functions of UCP2 in mitochondria, with particular emphasis on its intricate role in regulating mitochondrial calcium (Ca2+) uptake. Calcium 182-189 uncoupling protein 2 Homo sapiens 85-89 32492982-0 2020 Uncoupling protein 2 as genetic risk factor for Systemic Lupus Erythematosus: association with malondialdehyde levels and intima media thickness. Malondialdehyde 95-110 uncoupling protein 2 Homo sapiens 0-20 32492982-9 2020 CONCLUSIONS: Our results suggest that -866G/A UCP2 polymorphism is associated with SLE causing increased ROS production that, in turn, result in increased MDA levels responsible of accelerated atherosclerosis. Reactive Oxygen Species 105-108 uncoupling protein 2 Homo sapiens 46-50 33230296-0 2020 KRAS-regulated glutamine metabolism requires UCP2-mediated aspartate transport to support pancreatic cancer growth. Glutamine 15-24 uncoupling protein 2 Homo sapiens 45-49 33230296-0 2020 KRAS-regulated glutamine metabolism requires UCP2-mediated aspartate transport to support pancreatic cancer growth. Aspartic Acid 59-68 uncoupling protein 2 Homo sapiens 45-49 33230296-5 2020 UCP2-silenced KRASmut cell lines display decreased glutaminolysis, lower NADPH/NADP+ and glutathione/glutathione disulfide ratios and higher reactive oxygen species levels compared to wild-type counterparts. NADP 73-78 uncoupling protein 2 Homo sapiens 0-4 33230296-5 2020 UCP2-silenced KRASmut cell lines display decreased glutaminolysis, lower NADPH/NADP+ and glutathione/glutathione disulfide ratios and higher reactive oxygen species levels compared to wild-type counterparts. NADP 79-84 uncoupling protein 2 Homo sapiens 0-4 33230296-5 2020 UCP2-silenced KRASmut cell lines display decreased glutaminolysis, lower NADPH/NADP+ and glutathione/glutathione disulfide ratios and higher reactive oxygen species levels compared to wild-type counterparts. Glutathione 89-100 uncoupling protein 2 Homo sapiens 0-4 33230296-5 2020 UCP2-silenced KRASmut cell lines display decreased glutaminolysis, lower NADPH/NADP+ and glutathione/glutathione disulfide ratios and higher reactive oxygen species levels compared to wild-type counterparts. Glutathione 101-112 uncoupling protein 2 Homo sapiens 0-4 33230296-5 2020 UCP2-silenced KRASmut cell lines display decreased glutaminolysis, lower NADPH/NADP+ and glutathione/glutathione disulfide ratios and higher reactive oxygen species levels compared to wild-type counterparts. Disulfides 113-122 uncoupling protein 2 Homo sapiens 0-4 33230296-5 2020 UCP2-silenced KRASmut cell lines display decreased glutaminolysis, lower NADPH/NADP+ and glutathione/glutathione disulfide ratios and higher reactive oxygen species levels compared to wild-type counterparts. Oxygen 150-156 uncoupling protein 2 Homo sapiens 0-4 33230296-8 2020 Collectively, these results demonstrate that UCP2 plays a vital role in PDAC, since its aspartate transport activity connects the mitochondrial and cytosolic reactions necessary for KRASmut rewired glutamine metabolism2, and thus it should be considered a key metabolic target for the treatment of this refractory tumour. Aspartic Acid 88-97 uncoupling protein 2 Homo sapiens 45-49 33230296-8 2020 Collectively, these results demonstrate that UCP2 plays a vital role in PDAC, since its aspartate transport activity connects the mitochondrial and cytosolic reactions necessary for KRASmut rewired glutamine metabolism2, and thus it should be considered a key metabolic target for the treatment of this refractory tumour. Glutamine 198-207 uncoupling protein 2 Homo sapiens 45-49 33463495-1 2020 OBJECTIVE: To investigate whether the overexpression of uncoupling protein 2 (UCP2) can protect myocardium from sepsis by inhibiting the production of reactive oxygen species (ROS) and inflammatory response. Reactive Oxygen Species 151-174 uncoupling protein 2 Homo sapiens 56-76 33463495-1 2020 OBJECTIVE: To investigate whether the overexpression of uncoupling protein 2 (UCP2) can protect myocardium from sepsis by inhibiting the production of reactive oxygen species (ROS) and inflammatory response. Reactive Oxygen Species 151-174 uncoupling protein 2 Homo sapiens 78-82 33463495-1 2020 OBJECTIVE: To investigate whether the overexpression of uncoupling protein 2 (UCP2) can protect myocardium from sepsis by inhibiting the production of reactive oxygen species (ROS) and inflammatory response. Reactive Oxygen Species 176-179 uncoupling protein 2 Homo sapiens 56-76 33463495-1 2020 OBJECTIVE: To investigate whether the overexpression of uncoupling protein 2 (UCP2) can protect myocardium from sepsis by inhibiting the production of reactive oxygen species (ROS) and inflammatory response. Reactive Oxygen Species 176-179 uncoupling protein 2 Homo sapiens 78-82 33463495-11 2020 Under the fluorescence microscope, ROS production in the CLP and AAV groups were found significantly increased compared with that in the Sham group; when UCP2 was overexpressed, ROS production were significantly decreased compared with the CLP and AAV groups (A value: 1.03+-0.10 vs. 1.81+-0.13, 1.67+-0.08, both P < 0.01). Reactive Oxygen Species 178-181 uncoupling protein 2 Homo sapiens 154-158 33013386-11 2020 Moreover, AU reduced ischemia-reperfusion-induced mitochondrial dysfunction and cells apoptosis, increased peroxisome proliferator-activated receptor gamma coactivator (PGC)-1alpha and uncoupling (UCP)2 protein expression, and reduced caspase-3, cleaved caspase-3, and Cytochrome P450 proteins (CYP) expression. aucubin 10-12 uncoupling protein 2 Homo sapiens 197-202 32682215-0 2020 Neuroprotective and neuro-survival properties of safinamide against methamphetamine-induced neurodegeneration: Hypothetic possible role of BDNF/TrkB/PGC-1alpha signaling pathway and mitochondrial uncoupling protein -2(UCP-2). safinamide 49-59 uncoupling protein 2 Homo sapiens 182-217 32682215-0 2020 Neuroprotective and neuro-survival properties of safinamide against methamphetamine-induced neurodegeneration: Hypothetic possible role of BDNF/TrkB/PGC-1alpha signaling pathway and mitochondrial uncoupling protein -2(UCP-2). safinamide 49-59 uncoupling protein 2 Homo sapiens 218-223 32682215-5 2020 Neuroprotective strategies and approaches to the management, treatment or prevention of methamphetamine-induced neurodegeneration by modulating BDNF / TrkB / PGC-1alpha-UCP-2 can be considered as novel therapeutic approaches to these psychostimulant neurochemical and neurobehavioral approaches. Methamphetamine 88-103 uncoupling protein 2 Homo sapiens 169-174 32682215-7 2020 Although there is some evidence that BDNF / TrkB / PGC-1alpha-UCP-2 signaling pathway and mitochondrial UCP-2 mediated safinamide induced neuroprotection but it"s exact and precise mechanism of action and neuroprotective effects in neurodegenerative disorder and the protective properties against methamphetamine induced neurodegeneration and the role of BDNF / TrkB / PGC-1alpha signaling pathway and role of mitochondrial UCP-2 in this process have not yet been clarified. safinamide 119-129 uncoupling protein 2 Homo sapiens 104-109 32682215-7 2020 Although there is some evidence that BDNF / TrkB / PGC-1alpha-UCP-2 signaling pathway and mitochondrial UCP-2 mediated safinamide induced neuroprotection but it"s exact and precise mechanism of action and neuroprotective effects in neurodegenerative disorder and the protective properties against methamphetamine induced neurodegeneration and the role of BDNF / TrkB / PGC-1alpha signaling pathway and role of mitochondrial UCP-2 in this process have not yet been clarified. safinamide 119-129 uncoupling protein 2 Homo sapiens 104-109 32840960-7 2020 Loss of complex I was associated with disturbed NAD+ metabolism with increased UCP2 expression and reduced phosphorylated SirT1. NAD 48-52 uncoupling protein 2 Homo sapiens 79-83 31479096-1 2020 Uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 38-61 uncoupling protein 2 Homo sapiens 0-20 32470336-1 2020 Uncoupling protein-2 (UCP2) is a mitochondrial inner membrane anion carrier and is emerging as a negative regulator of ROS production. ros 119-122 uncoupling protein 2 Homo sapiens 0-20 32470336-1 2020 Uncoupling protein-2 (UCP2) is a mitochondrial inner membrane anion carrier and is emerging as a negative regulator of ROS production. ros 119-122 uncoupling protein 2 Homo sapiens 22-26 32217148-0 2020 Oleoylethanolamide supplementation in obese patients newly diagnosed with non-alcoholic fatty liver disease: effects on metabolic parameters, anthropometric indices, and expression of PPAR-alpha, UCP1, and UCP2 genes. oleoylethanolamide 0-18 uncoupling protein 2 Homo sapiens 206-210 32217148-2 2020 The objective of the present study was to examine the effects of OEA supplementation along with weight loss intervention on the expression of PPAR-alpha, uncoupling proteins 1and 2 (UCP1 and UCP2) genes in the peripheral blood mononuclear cells (PBMCs), metabolic parameters, and anthropometric indices among obese patients with NAFLD. oleoylethanolamide 65-68 uncoupling protein 2 Homo sapiens 191-195 32674299-5 2020 The most important intermediates by which polyphenols exert their protective effect include Bcl-2, UCP2, SIRT-1, AMPK and JNK1. Polyphenols 42-53 uncoupling protein 2 Homo sapiens 99-103 32654534-0 2020 Fish oil and chicoric acid combination protects better against palmitate-induced lipid accumulation via regulating AMPK-mediated SREBP-1/FAS and PPARalpha/UCP2 pathways. Fish Oils 0-8 uncoupling protein 2 Homo sapiens 155-159 32654534-0 2020 Fish oil and chicoric acid combination protects better against palmitate-induced lipid accumulation via regulating AMPK-mediated SREBP-1/FAS and PPARalpha/UCP2 pathways. chicoric acid 13-26 uncoupling protein 2 Homo sapiens 155-159 32654534-0 2020 Fish oil and chicoric acid combination protects better against palmitate-induced lipid accumulation via regulating AMPK-mediated SREBP-1/FAS and PPARalpha/UCP2 pathways. Palmitates 63-72 uncoupling protein 2 Homo sapiens 155-159 32319575-7 2020 Knockdown of UCP2 suppressed cell proliferation, invasion and spheroid formation, and increased the sensitivity of melanoma cells to cisplatin. Cisplatin 133-142 uncoupling protein 2 Homo sapiens 13-17 31479096-1 2020 Uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 38-61 uncoupling protein 2 Homo sapiens 22-26 31479096-1 2020 Uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 63-66 uncoupling protein 2 Homo sapiens 0-20 31479096-1 2020 Uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 63-66 uncoupling protein 2 Homo sapiens 22-26 32213883-3 2020 Results showed that Seleno-L-methionine did not cause an increase in hydrogen peroxide production at relatively low concentrations, accompanied by a rise in the antioxidant enzymes catalase and MnSOD, and UCP2 protein expression levels. Selenomethionine 20-39 uncoupling protein 2 Homo sapiens 205-209 32213883-6 2020 Moreover, at 10 microM, Seleno-L-cystine decreased UCP2 and MnSOD protein expression. L-Selenocystine 24-40 uncoupling protein 2 Homo sapiens 51-55 31811866-0 2020 UCP2 promotes proliferation and chemoresistance through regulating the NF-kappaB/beta-catenin axis and mitochondrial ROS in gallbladder cancer. ros 117-120 uncoupling protein 2 Homo sapiens 0-4 32244176-8 2020 Bortezomib and carfilzomib caused downregulation of the contents of mitochondrial oxidative phosphorylation complexes, voltage-dependent anion channel 1 (VDAC1) and uncoupling protein 2 (UCP2) similarly. Bortezomib 0-10 uncoupling protein 2 Homo sapiens 165-185 32244176-8 2020 Bortezomib and carfilzomib caused downregulation of the contents of mitochondrial oxidative phosphorylation complexes, voltage-dependent anion channel 1 (VDAC1) and uncoupling protein 2 (UCP2) similarly. Bortezomib 0-10 uncoupling protein 2 Homo sapiens 187-191 32244176-8 2020 Bortezomib and carfilzomib caused downregulation of the contents of mitochondrial oxidative phosphorylation complexes, voltage-dependent anion channel 1 (VDAC1) and uncoupling protein 2 (UCP2) similarly. carfilzomib 15-26 uncoupling protein 2 Homo sapiens 165-185 32244176-8 2020 Bortezomib and carfilzomib caused downregulation of the contents of mitochondrial oxidative phosphorylation complexes, voltage-dependent anion channel 1 (VDAC1) and uncoupling protein 2 (UCP2) similarly. carfilzomib 15-26 uncoupling protein 2 Homo sapiens 187-191 32190174-0 2020 Inhibition of Uncoupling Protein 2 Enhances the Radiosensitivity of Cervical Cancer Cells by Promoting the Production of Reactive Oxygen Species. Reactive Oxygen Species 121-144 uncoupling protein 2 Homo sapiens 14-34 32190174-8 2020 UCP2 silencing sensitized HeLa cells to irradiation-induced DNA damage and led to increased apoptosis, cell cycle arrest in G2/M, and increased mitochondrial ROS. Reactive Oxygen Species 158-161 uncoupling protein 2 Homo sapiens 0-4 31862780-6 2020 Mechanistically, ROS promoted uncoupling protein 2 (UCP2) protein expression and phosphorylation of AMPK, upregulating the expression of a key regulatory glycolytic enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3). Reactive Oxygen Species 17-20 uncoupling protein 2 Homo sapiens 30-50 31862780-6 2020 Mechanistically, ROS promoted uncoupling protein 2 (UCP2) protein expression and phosphorylation of AMPK, upregulating the expression of a key regulatory glycolytic enzyme, 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3). Reactive Oxygen Species 17-20 uncoupling protein 2 Homo sapiens 52-56 32120777-10 2020 The results of this study show that UCP-2 seems to impact the cardiac glucose metabolism during the transition from hypertrophy to failure by affecting glucose uptake through Glut-4. Glucose 70-77 uncoupling protein 2 Homo sapiens 36-41 32120777-11 2020 We suggest that the failing heart could benefit from low UCP-2 levels by improving the efficiency of glucose oxidation. Glucose 101-108 uncoupling protein 2 Homo sapiens 57-62 31811866-7 2020 More importantly, gallbladder cancer cells became sensitive to gemcitabine treatments when UCP2 was inhibited. gemcitabine 63-74 uncoupling protein 2 Homo sapiens 91-95 31811866-8 2020 UCP2 knockdown suppressed the activation of the NF-kappaB/beta-catenin axis and promoted the increases in mitochondrial ROS in gallbladder cancer cells exposed to gemcitabine treatments. ros 120-123 uncoupling protein 2 Homo sapiens 0-4 31811866-8 2020 UCP2 knockdown suppressed the activation of the NF-kappaB/beta-catenin axis and promoted the increases in mitochondrial ROS in gallbladder cancer cells exposed to gemcitabine treatments. gemcitabine 163-174 uncoupling protein 2 Homo sapiens 0-4 31811866-9 2020 The UCP2 inhibitor genipin suppressed xenograft tumor growth and sensitized grafted tumors to gemcitabine treatments. gemcitabine 94-105 uncoupling protein 2 Homo sapiens 4-8 32425675-3 2020 Uncoupling protein-2 (UCP2) is a mitochondrial transporter protein that controls M1 macrophage activation by modulating reactive oxygen species (ROS) production. Reactive Oxygen Species 120-143 uncoupling protein 2 Homo sapiens 0-20 32425675-3 2020 Uncoupling protein-2 (UCP2) is a mitochondrial transporter protein that controls M1 macrophage activation by modulating reactive oxygen species (ROS) production. Reactive Oxygen Species 120-143 uncoupling protein 2 Homo sapiens 22-26 32425675-3 2020 Uncoupling protein-2 (UCP2) is a mitochondrial transporter protein that controls M1 macrophage activation by modulating reactive oxygen species (ROS) production. Reactive Oxygen Species 145-148 uncoupling protein 2 Homo sapiens 0-20 32425675-3 2020 Uncoupling protein-2 (UCP2) is a mitochondrial transporter protein that controls M1 macrophage activation by modulating reactive oxygen species (ROS) production. Reactive Oxygen Species 145-148 uncoupling protein 2 Homo sapiens 22-26 33372430-10 2020 While no relationship was found between the UCP 1 and 3 genotypes and glucose/insulin levels during OGTT, carriers of the Insertion allele with UCP2 Ins/Del polymorphism had significantly higher 30-minute insulin levels (p=0.018). Glucose 70-77 uncoupling protein 2 Homo sapiens 144-148 33372430-12 2020 However, the presence of the Ins allele of the UCP2 gene has been found to have an unfavorable influence on early insulin excursion after glucose loading. Glucose 138-145 uncoupling protein 2 Homo sapiens 47-51 31250660-10 2019 Moreover, irisin upregulated UCP2 expression in the pancreas, and administration of genipin, a specific UCP2 antagonist, abolished irisin"s beneficial effects in L-arginine-induced AP. genipin 84-91 uncoupling protein 2 Homo sapiens 104-108 31699970-8 2019 Increasing mitochondrial ROS by inhibition or knock-down of the ROS-protective uncoupling protein UCP2 enhances cisplatin induced apoptosis. Cisplatin 112-121 uncoupling protein 2 Homo sapiens 98-102 31618075-9 2019 Inhibiting UCP2 with genipin sensitized HepG2 cells to palmitoylcarnitine, suggesting that activation of UCP2 may be a 2nd redox-based mechanism conferring protection. genipin 21-28 uncoupling protein 2 Homo sapiens 11-15 31618075-9 2019 Inhibiting UCP2 with genipin sensitized HepG2 cells to palmitoylcarnitine, suggesting that activation of UCP2 may be a 2nd redox-based mechanism conferring protection. Palmitoylcarnitine 55-73 uncoupling protein 2 Homo sapiens 11-15 31618075-9 2019 Inhibiting UCP2 with genipin sensitized HepG2 cells to palmitoylcarnitine, suggesting that activation of UCP2 may be a 2nd redox-based mechanism conferring protection. Palmitoylcarnitine 55-73 uncoupling protein 2 Homo sapiens 105-109 31618075-10 2019 These findings suggest that HepG2 cells possess inherent metabolic and redox flexibility relative to HT29 cells that confers protection from palmitoylcarnitine-induced stress via adaptive increases in mitochondrial respiratory control, glutathione buffering, and induction of UCP2. Palmitoylcarnitine 141-159 uncoupling protein 2 Homo sapiens 276-280 31218541-0 2019 The A allele of the UCP2 -866G/A polymorphism changes UCP2 promoter activity in HUVECs treated with high glucose. Glucose 105-112 uncoupling protein 2 Homo sapiens 20-24 31218541-0 2019 The A allele of the UCP2 -866G/A polymorphism changes UCP2 promoter activity in HUVECs treated with high glucose. Glucose 105-112 uncoupling protein 2 Homo sapiens 54-58 31218541-1 2019 The mitochondrial uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 56-79 uncoupling protein 2 Homo sapiens 4-38 31218541-1 2019 The mitochondrial uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 56-79 uncoupling protein 2 Homo sapiens 40-44 31218541-1 2019 The mitochondrial uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 81-84 uncoupling protein 2 Homo sapiens 4-38 31218541-1 2019 The mitochondrial uncoupling protein 2 (UCP2) decreases reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 81-84 uncoupling protein 2 Homo sapiens 40-44 31218541-4 2019 Thus, we investigated the effect of the A allele on UCP2 promoter activity in HUVECs treated with high glucose (HG) or hydrogen peroxide (H2O2). Glucose 103-110 uncoupling protein 2 Homo sapiens 52-56 31218541-4 2019 Thus, we investigated the effect of the A allele on UCP2 promoter activity in HUVECs treated with high glucose (HG) or hydrogen peroxide (H2O2). Hydrogen Peroxide 119-136 uncoupling protein 2 Homo sapiens 52-56 31218541-7 2019 HG induced an upregulation of UCP2 promoter activity in PGL3-866G cells after 24 h of treatment (P = 0.027), but not after 48 h. Compared to pGL3-866G cells, pGL3-866A cells seems to have reduced UCP2 promoter activity following 24 h and 48 h of normal glucose treatment (P = 0.087 and P = 0.022). Glucose 253-260 uncoupling protein 2 Homo sapiens 30-34 31218541-9 2019 Both pGL3-866G and pGL3-866A cells treated with H2O2 showed a 4-fold increase in UCP2 promoter activity (both P < 0.001). Hydrogen Peroxide 48-52 uncoupling protein 2 Homo sapiens 83-87 31218541-10 2019 The -866A allele modifies UCP2 promoter activity in HUVECs under HG treatment but not in the H2O2 condition. Mercury 65-67 uncoupling protein 2 Homo sapiens 26-30 31028741-4 2019 These effects were associated with an upregulation of uncoupling protein 2 (UCP2) and the activation of its upstream Sirtuin 1 (SIRT1)/(Liver kinase B1) LKB1- (Adenosine monophosphate-activated protein kinase) AMPK axis. Adenosine 160-169 uncoupling protein 2 Homo sapiens 54-74 31081966-8 2019 Finally, suppression of mitophagy with mdivi-1 in cultured cardiomyocytes abolished UCP2-afforded protective effect on sI/R-induced mitochondrial dysfunction and cell death. Silicon 119-121 uncoupling protein 2 Homo sapiens 84-88 31081966-8 2019 Finally, suppression of mitophagy with mdivi-1 in cultured cardiomyocytes abolished UCP2-afforded protective effect on sI/R-induced mitochondrial dysfunction and cell death. r 122-123 uncoupling protein 2 Homo sapiens 84-88 31105116-6 2019 The ATP decrease, MMP loss, Ca2+ increase, the activation of uncoupling protein-2 (UCP-2) and calpain-1 were significant after DMF exposure. Dimethylformamide 127-130 uncoupling protein 2 Homo sapiens 61-81 31105116-6 2019 The ATP decrease, MMP loss, Ca2+ increase, the activation of uncoupling protein-2 (UCP-2) and calpain-1 were significant after DMF exposure. Dimethylformamide 127-130 uncoupling protein 2 Homo sapiens 83-88 31771728-15 2019 (4) The results of immunofluorescence showed that the ATP content of CLP group and AAV group were significantly lower than that of Sham group; the ATP content of UCP2 group was significantly higher than that of CLP group and AAV group (mumol/L: 1.99+-0.15 vs. 1.10+-0.17, 1.13+-0.19, both P < 0.05). Adenosine Triphosphate 147-150 uncoupling protein 2 Homo sapiens 162-166 31028741-4 2019 These effects were associated with an upregulation of uncoupling protein 2 (UCP2) and the activation of its upstream Sirtuin 1 (SIRT1)/(Liver kinase B1) LKB1- (Adenosine monophosphate-activated protein kinase) AMPK axis. Adenosine 160-169 uncoupling protein 2 Homo sapiens 76-80 31028741-5 2019 The pivotal role of UCP2 in the cancer-suppressing effect was demonstrated by overexpressing UCP2 in HCT-116 cells with similar metabolic effects to those produced by Bou. bouchardatine 167-170 uncoupling protein 2 Homo sapiens 20-24 30995317-1 2019 Purpose: We address the hypothesis that uncoupling protein 2 (UCP2), a cellular glucose regulator, delays physiologic retinal vascular development (PRVD) by interfering with glucose uptake through glucose transporter 1 (Glut1). Glucose 80-87 uncoupling protein 2 Homo sapiens 40-60 31341797-3 2019 UCP2 mRNA expression in cells treated with H2O2 was investigated by reverse transcription-polymerase chain reaction (RT-PCR). Hydrogen Peroxide 43-47 uncoupling protein 2 Homo sapiens 0-4 31341797-5 2019 Further, UCP2-siRNA treated cultures were exposed to H2O2 (0, 75, 150, and 300 micromol/L) for 2h and cell viability determined by MTT assay. Hydrogen Peroxide 53-57 uncoupling protein 2 Homo sapiens 9-13 31341797-5 2019 Further, UCP2-siRNA treated cultures were exposed to H2O2 (0, 75, 150, and 300 micromol/L) for 2h and cell viability determined by MTT assay. Deuterium 95-97 uncoupling protein 2 Homo sapiens 9-13 31341797-5 2019 Further, UCP2-siRNA treated cultures were exposed to H2O2 (0, 75, 150, and 300 micromol/L) for 2h and cell viability determined by MTT assay. monooxyethylene trimethylolpropane tristearate 131-134 uncoupling protein 2 Homo sapiens 9-13 31341797-10 2019 Levels of OS were further decreased in cells treated with UCP2-siRNA compared with those treated with H2O2 alone (P<0.05). Hydrogen Peroxide 102-106 uncoupling protein 2 Homo sapiens 58-62 31341797-11 2019 The results of RT-PCR and Western blotting demonstrated that UCP2 expression was reduced in H2O2-treated groups compared with controls (P<0.05). Hydrogen Peroxide 92-96 uncoupling protein 2 Homo sapiens 61-65 31341797-12 2019 FCM analysis showed that cell reactive oxygen species (ROS) levels were increased in H2O2-treated groups and further upregulated by UCP2-siRNA treatment (P<0.05). Reactive Oxygen Species 30-53 uncoupling protein 2 Homo sapiens 132-136 31341797-12 2019 FCM analysis showed that cell reactive oxygen species (ROS) levels were increased in H2O2-treated groups and further upregulated by UCP2-siRNA treatment (P<0.05). Reactive Oxygen Species 55-58 uncoupling protein 2 Homo sapiens 132-136 31341797-12 2019 FCM analysis showed that cell reactive oxygen species (ROS) levels were increased in H2O2-treated groups and further upregulated by UCP2-siRNA treatment (P<0.05). Hydrogen Peroxide 85-89 uncoupling protein 2 Homo sapiens 132-136 31341797-13 2019 CONCLUSION: Expression levels of UCP2 are decreased in ARPE-19 cells treated with H2O2. Hydrogen Peroxide 82-86 uncoupling protein 2 Homo sapiens 33-37 31341797-14 2019 ROS levels are further increased in cells treated with UCP2-siRNA relative to those treated with H2O2 alone. Reactive Oxygen Species 0-3 uncoupling protein 2 Homo sapiens 55-59 31341797-14 2019 ROS levels are further increased in cells treated with UCP2-siRNA relative to those treated with H2O2 alone. Hydrogen Peroxide 97-101 uncoupling protein 2 Homo sapiens 55-59 31275989-13 2019 Conclusions: siVDR, AKT inhibitor, and UCP2 inhibitor elevated the ROS and apoptosis of HK2 cells while attenuating the mitochondrial membrane potential, suggesting that vitamin D protects renal tubular cell from high glucose by AKT/UCP2 signaling pathway. Vitamin D 170-179 uncoupling protein 2 Homo sapiens 39-43 31275989-13 2019 Conclusions: siVDR, AKT inhibitor, and UCP2 inhibitor elevated the ROS and apoptosis of HK2 cells while attenuating the mitochondrial membrane potential, suggesting that vitamin D protects renal tubular cell from high glucose by AKT/UCP2 signaling pathway. Vitamin D 170-179 uncoupling protein 2 Homo sapiens 233-237 31275989-13 2019 Conclusions: siVDR, AKT inhibitor, and UCP2 inhibitor elevated the ROS and apoptosis of HK2 cells while attenuating the mitochondrial membrane potential, suggesting that vitamin D protects renal tubular cell from high glucose by AKT/UCP2 signaling pathway. Glucose 218-225 uncoupling protein 2 Homo sapiens 39-43 31071510-0 2019 UCP2 alleviates tubular epithelial cell apoptosis in lipopolysaccharide-induced acute kidney injury by decreasing ROS production. Reactive Oxygen Species 114-117 uncoupling protein 2 Homo sapiens 0-4 31071510-1 2019 Uncoupling protein 2 (UCP2), an anion transporter, modulates the production of mitochondrial reactive oxygen species (ROS) and plays an important role in protecting against cell apoptosis. Reactive Oxygen Species 93-116 uncoupling protein 2 Homo sapiens 0-20 31071510-1 2019 Uncoupling protein 2 (UCP2), an anion transporter, modulates the production of mitochondrial reactive oxygen species (ROS) and plays an important role in protecting against cell apoptosis. Reactive Oxygen Species 93-116 uncoupling protein 2 Homo sapiens 22-26 31071510-1 2019 Uncoupling protein 2 (UCP2), an anion transporter, modulates the production of mitochondrial reactive oxygen species (ROS) and plays an important role in protecting against cell apoptosis. Reactive Oxygen Species 118-121 uncoupling protein 2 Homo sapiens 0-20 31071510-1 2019 Uncoupling protein 2 (UCP2), an anion transporter, modulates the production of mitochondrial reactive oxygen species (ROS) and plays an important role in protecting against cell apoptosis. Reactive Oxygen Species 118-121 uncoupling protein 2 Homo sapiens 22-26 31071510-6 2019 Furthermore, UCP2 silencing dramatically aggravated LPS-induced apoptosis, accompanied by increased ROS production in renal tubular epithelial cell. Reactive Oxygen Species 100-103 uncoupling protein 2 Homo sapiens 13-17 31071510-8 2019 Moreover, NAC (N-acetylcysteine), a potent ROS scavenger, obviously suppressed apoptosis induced by UCP2 silencing, which suggests that the increased ROS levels were associated with tubular epithelial cell apoptosis induced by UCP2 silencing. Acetylcysteine 10-13 uncoupling protein 2 Homo sapiens 100-104 31071510-8 2019 Moreover, NAC (N-acetylcysteine), a potent ROS scavenger, obviously suppressed apoptosis induced by UCP2 silencing, which suggests that the increased ROS levels were associated with tubular epithelial cell apoptosis induced by UCP2 silencing. Acetylcysteine 10-13 uncoupling protein 2 Homo sapiens 227-231 31071510-8 2019 Moreover, NAC (N-acetylcysteine), a potent ROS scavenger, obviously suppressed apoptosis induced by UCP2 silencing, which suggests that the increased ROS levels were associated with tubular epithelial cell apoptosis induced by UCP2 silencing. Acetylcysteine 15-31 uncoupling protein 2 Homo sapiens 100-104 31071510-8 2019 Moreover, NAC (N-acetylcysteine), a potent ROS scavenger, obviously suppressed apoptosis induced by UCP2 silencing, which suggests that the increased ROS levels were associated with tubular epithelial cell apoptosis induced by UCP2 silencing. Acetylcysteine 15-31 uncoupling protein 2 Homo sapiens 227-231 31071510-8 2019 Moreover, NAC (N-acetylcysteine), a potent ROS scavenger, obviously suppressed apoptosis induced by UCP2 silencing, which suggests that the increased ROS levels were associated with tubular epithelial cell apoptosis induced by UCP2 silencing. Reactive Oxygen Species 150-153 uncoupling protein 2 Homo sapiens 100-104 31071510-8 2019 Moreover, NAC (N-acetylcysteine), a potent ROS scavenger, obviously suppressed apoptosis induced by UCP2 silencing, which suggests that the increased ROS levels were associated with tubular epithelial cell apoptosis induced by UCP2 silencing. Reactive Oxygen Species 150-153 uncoupling protein 2 Homo sapiens 227-231 31071510-9 2019 Therefore, UCP2 exerts a protective effect on the LPS-induced apoptosis of tubular epithelial cells by reducing excess ROS production. Reactive Oxygen Species 119-122 uncoupling protein 2 Homo sapiens 11-15 30910560-2 2019 Uncoupling protein-2 (UCP2) is involved in the regulation of reactive oxygen species production, insulin secretion, and lipid metabolism. Reactive Oxygen Species 61-84 uncoupling protein 2 Homo sapiens 0-20 30910560-2 2019 Uncoupling protein-2 (UCP2) is involved in the regulation of reactive oxygen species production, insulin secretion, and lipid metabolism. Reactive Oxygen Species 61-84 uncoupling protein 2 Homo sapiens 22-26 31249597-3 2019 In this context, miR-15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Reactive Oxygen Species 161-184 uncoupling protein 2 Homo sapiens 93-113 31249597-3 2019 In this context, miR-15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Reactive Oxygen Species 161-184 uncoupling protein 2 Homo sapiens 115-119 31249597-3 2019 In this context, miR-15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Reactive Oxygen Species 186-189 uncoupling protein 2 Homo sapiens 93-113 31249597-3 2019 In this context, miR-15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Reactive Oxygen Species 186-189 uncoupling protein 2 Homo sapiens 115-119 30952098-8 2019 In vitro, UCP2 overexpression protected HK-2 cells from LPS-induced injury by suppression of apoptosis, inflammation, oxidative stress, MMP loss and ROS production, increase of ATP production and mtDNA content, and amelioration of damage to the mitochondrial ultrastructure. ros 149-152 uncoupling protein 2 Homo sapiens 10-14 30952098-8 2019 In vitro, UCP2 overexpression protected HK-2 cells from LPS-induced injury by suppression of apoptosis, inflammation, oxidative stress, MMP loss and ROS production, increase of ATP production and mtDNA content, and amelioration of damage to the mitochondrial ultrastructure. Adenosine Triphosphate 177-180 uncoupling protein 2 Homo sapiens 10-14 31275989-0 2019 Active Vitamin D and Vitamin D Receptor Help Prevent High Glucose Induced Oxidative Stress of Renal Tubular Cells via AKT/UCP2 Signaling Pathway. Vitamin D 7-16 uncoupling protein 2 Homo sapiens 122-126 31275989-2 2019 We here tested the hypothesis that active vitamin D is able to up-regulate AKT/UCP2 signaling to alleviate oxidative stress of renal tubular cell line HK2. Vitamin D 42-51 uncoupling protein 2 Homo sapiens 79-83 30885735-0 2019 Glutamine regulates mitochondrial uncoupling protein 2 to promote glutaminolysis in neuroblastoma cells. Glutamine 0-9 uncoupling protein 2 Homo sapiens 20-54 30885735-4 2019 We reveal that glutamine shortage induces the rapid and reversible downregulation of UCP2, decrease of the metabolic activity and proliferation of neuroblastoma cells, that are regulated by glutamine per se but not by glutamine metabolism. Glutamine 15-24 uncoupling protein 2 Homo sapiens 85-89 30885735-4 2019 We reveal that glutamine shortage induces the rapid and reversible downregulation of UCP2, decrease of the metabolic activity and proliferation of neuroblastoma cells, that are regulated by glutamine per se but not by glutamine metabolism. Glutamine 190-199 uncoupling protein 2 Homo sapiens 85-89 30885735-4 2019 We reveal that glutamine shortage induces the rapid and reversible downregulation of UCP2, decrease of the metabolic activity and proliferation of neuroblastoma cells, that are regulated by glutamine per se but not by glutamine metabolism. Glutamine 190-199 uncoupling protein 2 Homo sapiens 85-89 30885735-6 2019 The results imply that UCP2 facilitates glutamine utilization as an energetic fuel source, thereby providing metabolic flexibility during glucose shortage. Glutamine 40-49 uncoupling protein 2 Homo sapiens 23-27 30885735-6 2019 The results imply that UCP2 facilitates glutamine utilization as an energetic fuel source, thereby providing metabolic flexibility during glucose shortage. Glucose 138-145 uncoupling protein 2 Homo sapiens 23-27 30995317-1 2019 Purpose: We address the hypothesis that uncoupling protein 2 (UCP2), a cellular glucose regulator, delays physiologic retinal vascular development (PRVD) by interfering with glucose uptake through glucose transporter 1 (Glut1). Glucose 80-87 uncoupling protein 2 Homo sapiens 62-66 30995317-1 2019 Purpose: We address the hypothesis that uncoupling protein 2 (UCP2), a cellular glucose regulator, delays physiologic retinal vascular development (PRVD) by interfering with glucose uptake through glucose transporter 1 (Glut1). Glucose 174-181 uncoupling protein 2 Homo sapiens 40-60 30995317-1 2019 Purpose: We address the hypothesis that uncoupling protein 2 (UCP2), a cellular glucose regulator, delays physiologic retinal vascular development (PRVD) by interfering with glucose uptake through glucose transporter 1 (Glut1). Glucose 174-181 uncoupling protein 2 Homo sapiens 62-66 30995317-9 2019 Compared to control, treatment with genipin or knockdown of UCP2 significantly increased Glut1, glucose uptake, ATP production, VEGF-induced p-VEGFR2 and cell proliferation in hRMVECs. Glucose 96-103 uncoupling protein 2 Homo sapiens 60-64 30995317-9 2019 Compared to control, treatment with genipin or knockdown of UCP2 significantly increased Glut1, glucose uptake, ATP production, VEGF-induced p-VEGFR2 and cell proliferation in hRMVECs. Adenosine Triphosphate 112-115 uncoupling protein 2 Homo sapiens 60-64 30556651-0 2019 UCP2 Overexpression Redirects Glucose into Anabolic Metabolic Pathways. Glucose 30-37 uncoupling protein 2 Homo sapiens 0-4 20301549-12 1993 FHI-UCP2, caused by pathgoenic variants in UCP2, is a rare cause of diazoxide-responsive FH1. Diazoxide 68-77 uncoupling protein 2 Homo sapiens 4-8 20301549-12 1993 FHI-UCP2, caused by pathgoenic variants in UCP2, is a rare cause of diazoxide-responsive FH1. Diazoxide 68-77 uncoupling protein 2 Homo sapiens 43-47 30881027-10 2019 Mitochondrial uncoupling protein 2 (UCP2) protects against mitochondrial ROS while allowing energy metabolism to switch to glycolysis. ros 73-76 uncoupling protein 2 Homo sapiens 0-34 30881027-10 2019 Mitochondrial uncoupling protein 2 (UCP2) protects against mitochondrial ROS while allowing energy metabolism to switch to glycolysis. ros 73-76 uncoupling protein 2 Homo sapiens 36-40 30881027-11 2019 Treatment of A2780 cells with various concentrations of DCA resulted in decreased expression of UCP2, a metabolic switch from glycolysis to mitochondrial OXPHOS, and an increase in oxidative stress induced by ROS. Dichloroacetic Acid 56-59 uncoupling protein 2 Homo sapiens 96-100 30881027-12 2019 These effects were not observed in A2780/DDP cells with higher UCP2 expression suggesting that UCP2 might induce changes in mitochondrial functions that result in different sensitivities to DCA. Dichloroacetic Acid 190-193 uncoupling protein 2 Homo sapiens 95-99 30742657-0 2019 Melatonin decreases M1 polarization via attenuating mitochondrial oxidative damage depending on UCP2 pathway in prorenin-treated microglia. Melatonin 0-9 uncoupling protein 2 Homo sapiens 96-100 30742657-4 2019 In present study, we investigated the protective role of melatonin in decreasing M1 phenotype switching via attenuating mitochondrial oxidative damage in dependence on uncoupling protein 2 (UCP2) pathway in microglia. Melatonin 57-66 uncoupling protein 2 Homo sapiens 168-188 30742657-4 2019 In present study, we investigated the protective role of melatonin in decreasing M1 phenotype switching via attenuating mitochondrial oxidative damage in dependence on uncoupling protein 2 (UCP2) pathway in microglia. Melatonin 57-66 uncoupling protein 2 Homo sapiens 190-194 30742657-15 2019 Our results suggested that the protective effect of melatonin against prorenin-induced M1 phenotype switching via attenuating mitochondrial oxidative damage depending on UCP2 upregulation in prorenin-treated microglia. Melatonin 52-61 uncoupling protein 2 Homo sapiens 170-174 30556651-3 2019 To determine the global metabolic impact of UCP2 upregulation, 13 C6 glucose as a source molecule is used to "trace" the metabolic fate of carbon atoms derived from glucose. Glucose 69-76 uncoupling protein 2 Homo sapiens 44-48 30556651-3 2019 To determine the global metabolic impact of UCP2 upregulation, 13 C6 glucose as a source molecule is used to "trace" the metabolic fate of carbon atoms derived from glucose. Carbon 139-145 uncoupling protein 2 Homo sapiens 44-48 30556651-3 2019 To determine the global metabolic impact of UCP2 upregulation, 13 C6 glucose as a source molecule is used to "trace" the metabolic fate of carbon atoms derived from glucose. Glucose 165-172 uncoupling protein 2 Homo sapiens 44-48 30556651-4 2019 UCP2 overexpression in skin epidermal cells enhances the incorporation of 13 C label to pyruvate, tricarboxylic acid cycle intermediates, nucleotides, and amino acids, suggesting that UCP2 upregulation reprograms cellular metabolism toward macromolecule synthesis. Pyruvic Acid 88-96 uncoupling protein 2 Homo sapiens 0-4 30556651-4 2019 UCP2 overexpression in skin epidermal cells enhances the incorporation of 13 C label to pyruvate, tricarboxylic acid cycle intermediates, nucleotides, and amino acids, suggesting that UCP2 upregulation reprograms cellular metabolism toward macromolecule synthesis. Tricarboxylic Acids 98-116 uncoupling protein 2 Homo sapiens 0-4 30431098-9 2019 Significant differences were demonstrated in the levels of UCP2 gene expression between the PEDF+ H2O2 treated group and cells treated with H2O2 alone. Hydrogen Peroxide 98-102 uncoupling protein 2 Homo sapiens 59-63 30431098-9 2019 Significant differences were demonstrated in the levels of UCP2 gene expression between the PEDF+ H2O2 treated group and cells treated with H2O2 alone. Hydrogen Peroxide 140-144 uncoupling protein 2 Homo sapiens 59-63 30431098-10 2019 Labeling of the UCP2 detector in the confocal images demonstrated decreased UCP2 protein staining in the retinal pigment epithelium (RPE) cells and RPE layers following H2O2 injury; however, this effect was inhibited following treatment with PEDF. Hydrogen Peroxide 169-173 uncoupling protein 2 Homo sapiens 16-20 30431098-10 2019 Labeling of the UCP2 detector in the confocal images demonstrated decreased UCP2 protein staining in the retinal pigment epithelium (RPE) cells and RPE layers following H2O2 injury; however, this effect was inhibited following treatment with PEDF. Hydrogen Peroxide 169-173 uncoupling protein 2 Homo sapiens 76-80 30318520-0 2018 Mutant p53 blocks SESN1/AMPK/PGC-1alpha/UCP2 axis increasing mitochondrial O2- production in cancer cells. Oxygen 75-77 uncoupling protein 2 Homo sapiens 40-44 30027365-4 2019 We show that hippocampal pyramidal neurons in mutUNG1 mice, as well as cultured rat hippocampal neurons and human fibroblasts with H2O2 induced oxidative stress, improve markers of mitochondrial biogenesis, dynamics and function when fed on a KD, and when exposed to the ketone body beta-hydroxybutyrate, respectively, by upregulating PGC1alpha, SIRT3 and UCP2, and (in cultured cells) increasing the oxygen consumption rate (OCR) and the NAD+/NADH ratio. Hydrogen Peroxide 131-135 uncoupling protein 2 Homo sapiens 356-360 30538804-8 2018 The beta3-AR/UCP2 axis induces a strong reduction of mitochondrial activity by reducing ATP synthesis and mitochondrial reactive oxygen species (mtROS) content. Adenosine Triphosphate 88-91 uncoupling protein 2 Homo sapiens 13-17 30538804-8 2018 The beta3-AR/UCP2 axis induces a strong reduction of mitochondrial activity by reducing ATP synthesis and mitochondrial reactive oxygen species (mtROS) content. Reactive Oxygen Species 120-143 uncoupling protein 2 Homo sapiens 13-17 30538804-8 2018 The beta3-AR/UCP2 axis induces a strong reduction of mitochondrial activity by reducing ATP synthesis and mitochondrial reactive oxygen species (mtROS) content. mtros 145-150 uncoupling protein 2 Homo sapiens 13-17 30429902-4 2018 We found that the UCP2 Ala55Val polymorphism was associated with running performance, with the subjects carrying the Val allele being overrepresented in the group of most successful runners (<100 min) compared to the >100 min group (84.2 vs. 55.8%; OR = 4.23, p < 0.0001). Valine 28-31 uncoupling protein 2 Homo sapiens 18-22 30464607-15 2018 Both PRL-3 and RAP1 could regulate the expression of manganese superoxide dismutase 2 (SOD2) and the uncoupling protein 2 (UCP2), which may be related to PRL-3 suppression induced mitochondria superoxide anion. Superoxides 193-209 uncoupling protein 2 Homo sapiens 101-121 30464607-15 2018 Both PRL-3 and RAP1 could regulate the expression of manganese superoxide dismutase 2 (SOD2) and the uncoupling protein 2 (UCP2), which may be related to PRL-3 suppression induced mitochondria superoxide anion. Superoxides 193-209 uncoupling protein 2 Homo sapiens 123-127 30381728-1 2018 Ala55Val and 45 basepair (bp) insertion/deletion (I/D) of UCP2 gene polymorphisms cause a decrease in resting energy expenditure, decreasing fatty acid oxidation and influencing mRNA transcription and stability, thereby increasing the risk of obesity. Fatty Acids 141-151 uncoupling protein 2 Homo sapiens 58-62 29351723-4 2018 Recent Advances: UCP2 structure, including purine nucleotide and fatty acid (FA) binding sites, strongly support the FA cycling mechanism: UCP2 expels FA anions, whereas uncoupling is achieved by the membrane backflux of protonated FA. Purine Nucleotides 43-60 uncoupling protein 2 Homo sapiens 17-21 29351723-4 2018 Recent Advances: UCP2 structure, including purine nucleotide and fatty acid (FA) binding sites, strongly support the FA cycling mechanism: UCP2 expels FA anions, whereas uncoupling is achieved by the membrane backflux of protonated FA. Fatty Acids 65-75 uncoupling protein 2 Homo sapiens 17-21 29351723-8 2018 Moreover, UCP2-mediated aspartate, oxaloacetate, and malate antiport with phosphate is expected to alter metabolism of cancer cells. Aspartic Acid 24-33 uncoupling protein 2 Homo sapiens 10-14 29351723-8 2018 Moreover, UCP2-mediated aspartate, oxaloacetate, and malate antiport with phosphate is expected to alter metabolism of cancer cells. Oxaloacetic Acid 35-47 uncoupling protein 2 Homo sapiens 10-14 29351723-8 2018 Moreover, UCP2-mediated aspartate, oxaloacetate, and malate antiport with phosphate is expected to alter metabolism of cancer cells. malic acid 53-59 uncoupling protein 2 Homo sapiens 10-14 29351723-8 2018 Moreover, UCP2-mediated aspartate, oxaloacetate, and malate antiport with phosphate is expected to alter metabolism of cancer cells. Phosphates 74-83 uncoupling protein 2 Homo sapiens 10-14 29351723-10 2018 Switching off/on the UCP2 protonophoretic function might serve as redox signaling either by employing/releasing the extra capacity of cell antioxidant systems or by directly increasing/decreasing mitochondrial superoxide sources. Superoxides 210-220 uncoupling protein 2 Homo sapiens 21-25 29351723-12 2018 FUTURE DIRECTIONS: Issues such as UCP2 participation in glucose sensing, neuronal (synaptic) function, and immune cell activation should be elucidated. Glucose 56-63 uncoupling protein 2 Homo sapiens 34-38 29859845-5 2018 There is strong evidence that UCP2 and UCP3, the UCP1 homologues expressed in the heart, protect against mitochondrial oxidative damage by reducing the production of ROS. Reactive Oxygen Species 166-169 uncoupling protein 2 Homo sapiens 30-34 30116205-7 2018 In view of the different functions of UCP2 in various cell types that contribute to whole body homeostasis, cell type-specific modification of UCP2 expression may offer a better approach to help understanding how UCP2 governs mitochondrial function, reactive oxygen species production and transmembrane proton leak and how dysfunction of UCP2 participates in the development of cardiovascular diseases. Reactive Oxygen Species 250-273 uncoupling protein 2 Homo sapiens 143-147 30160798-1 2018 Genipin, a compound derived from Gardenis jasminoides Ellis fruits, was demonstrated to be the specific uncoupling protein 2 (UCP2) inhibitor. genipin 0-7 uncoupling protein 2 Homo sapiens 104-124 30160798-1 2018 Genipin, a compound derived from Gardenis jasminoides Ellis fruits, was demonstrated to be the specific uncoupling protein 2 (UCP2) inhibitor. genipin 0-7 uncoupling protein 2 Homo sapiens 126-130 30160798-2 2018 UCP2 is a mitochondrial carrier protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from adenosine triphosphate (ATP) synthesis. Adenosine Triphosphate 154-176 uncoupling protein 2 Homo sapiens 0-4 30160798-2 2018 UCP2 is a mitochondrial carrier protein that creates proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from adenosine triphosphate (ATP) synthesis. Adenosine Triphosphate 178-181 uncoupling protein 2 Homo sapiens 0-4 30160798-16 2018 In conclusion, the antiproliferative effects of genipin on the growth of both glioblastoma cell lines have been shown in all of these assays, and genipin profoundly induced apoptosis in both cell lines via the UCP2-related mitochondrial pathway through the induction of intracellular ROS. Reactive Oxygen Species 284-287 uncoupling protein 2 Homo sapiens 210-214 29878130-9 2018 Similarly, UCP2 and UCP3 protein levels were increased in differentiated adipocytes upon acute norepinephrine stimulation. Norepinephrine 95-109 uncoupling protein 2 Homo sapiens 11-15 30007886-6 2018 In addition, Aspergillus protease-mediated mitochondrial ROS production was associated with downregulation of uncoupling protein (UCP)- 2 expression by TGF-beta-SMAD4 signaling, which may play a regulatory role in mitochondrial ROS formation during fungal protease-mediated epithelial inflammation. Reactive Oxygen Species 57-60 uncoupling protein 2 Homo sapiens 110-137 30007886-6 2018 In addition, Aspergillus protease-mediated mitochondrial ROS production was associated with downregulation of uncoupling protein (UCP)- 2 expression by TGF-beta-SMAD4 signaling, which may play a regulatory role in mitochondrial ROS formation during fungal protease-mediated epithelial inflammation. Reactive Oxygen Species 228-231 uncoupling protein 2 Homo sapiens 110-137 30116205-7 2018 In view of the different functions of UCP2 in various cell types that contribute to whole body homeostasis, cell type-specific modification of UCP2 expression may offer a better approach to help understanding how UCP2 governs mitochondrial function, reactive oxygen species production and transmembrane proton leak and how dysfunction of UCP2 participates in the development of cardiovascular diseases. Reactive Oxygen Species 250-273 uncoupling protein 2 Homo sapiens 143-147 30116205-7 2018 In view of the different functions of UCP2 in various cell types that contribute to whole body homeostasis, cell type-specific modification of UCP2 expression may offer a better approach to help understanding how UCP2 governs mitochondrial function, reactive oxygen species production and transmembrane proton leak and how dysfunction of UCP2 participates in the development of cardiovascular diseases. Reactive Oxygen Species 250-273 uncoupling protein 2 Homo sapiens 143-147 29752946-14 2018 The UCP-2 gene showed a significant increase in proline and lysine treatment. Proline 48-55 uncoupling protein 2 Homo sapiens 4-9 29752946-14 2018 The UCP-2 gene showed a significant increase in proline and lysine treatment. Lysine 60-66 uncoupling protein 2 Homo sapiens 4-9 29845235-0 2018 Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR-gamma and UCP2. Rosuvastatin Calcium 0-12 uncoupling protein 2 Homo sapiens 92-96 29845235-9 2018 RS inhibited myocardial infarct size, downregulated expression of caspase-9 and cyt c and upregulated expression of UCP2 and PPAR-gamma by inhibiting ATR. Rosuvastatin Calcium 0-2 uncoupling protein 2 Homo sapiens 116-120 29845235-10 2018 Furthermore, the results indicated that RS promoted cardiomyocyte viability, inhibited LDH release, reduced ROS production, decreased expression of caspase-9 and cyt c, and increased expression of UCP2 and PPAR-gamma following OGD/R damage. Rosuvastatin Calcium 40-42 uncoupling protein 2 Homo sapiens 197-201 29634487-7 2018 Most of the significant differences in the clinical parameters were found under a recessive model, the UCP2 -866 polymorphism was associated with diastolic blood pressure (p=0.008), triglycerides (p=0.045), low-density lipoprotein-cholesterol (LDL-C) (p=0.003), high-density lipoprotein-cholesterol (HDL-C) (p=0.050) and plasma levels of leptin (p<0.001). Triglycerides 182-195 uncoupling protein 2 Homo sapiens 103-107 29928365-0 2018 Expression of UCP2 is associated with sensitivity to platinum-based chemotherapy for ovarian serous carcinoma. Platinum 53-61 uncoupling protein 2 Homo sapiens 14-18 29928365-4 2018 Uncoupling protein 2 (UCP2) is widely expressed in cancer cells and regulates the production of mitochondrial reactive oxygen species (ROS). Reactive Oxygen Species 110-133 uncoupling protein 2 Homo sapiens 0-20 29928365-4 2018 Uncoupling protein 2 (UCP2) is widely expressed in cancer cells and regulates the production of mitochondrial reactive oxygen species (ROS). Reactive Oxygen Species 110-133 uncoupling protein 2 Homo sapiens 22-26 29928365-4 2018 Uncoupling protein 2 (UCP2) is widely expressed in cancer cells and regulates the production of mitochondrial reactive oxygen species (ROS). Reactive Oxygen Species 135-138 uncoupling protein 2 Homo sapiens 0-20 29928365-4 2018 Uncoupling protein 2 (UCP2) is widely expressed in cancer cells and regulates the production of mitochondrial reactive oxygen species (ROS). Reactive Oxygen Species 135-138 uncoupling protein 2 Homo sapiens 22-26 29928365-7 2018 The present study investigated the association between UCP2 expression and platinum sensitivity. Platinum 75-83 uncoupling protein 2 Homo sapiens 55-59 29928365-11 2018 The UCP2 weighted score was lower in the platinum-sensitive group than in the platinum resistant-group (P=0.005). Platinum 41-49 uncoupling protein 2 Homo sapiens 4-8 29928365-11 2018 The UCP2 weighted score was lower in the platinum-sensitive group than in the platinum resistant-group (P=0.005). Platinum 78-86 uncoupling protein 2 Homo sapiens 4-8 29928365-12 2018 In addition, patients in the low UCP2 expression group were more sensitive to platinum-based chemotherapy than those in the high UCP2 expression group (P=0.001). Platinum 78-86 uncoupling protein 2 Homo sapiens 33-37 29928365-13 2018 Sensitivity to carboplatin was significantly increased when UCP2 was inhibited in human ovarian serous carcinoma cells in vitro. Carboplatin 15-26 uncoupling protein 2 Homo sapiens 60-64 29928365-14 2018 UCP2 expression may be a predictive marker of the efficacy of platinum-based chemotherapy for patients with ovarian serous carcinoma. Platinum 62-70 uncoupling protein 2 Homo sapiens 0-4 29861658-8 2018 Moreover, the effect of GA on the regulation of mitochondrial ROS depends on the expression of uncoupling protein-2 (UCP-2). 18alpha-glycyrrhetinic acid 24-26 uncoupling protein 2 Homo sapiens 95-115 29861658-8 2018 Moreover, the effect of GA on the regulation of mitochondrial ROS depends on the expression of uncoupling protein-2 (UCP-2). 18alpha-glycyrrhetinic acid 24-26 uncoupling protein 2 Homo sapiens 117-122 29861658-8 2018 Moreover, the effect of GA on the regulation of mitochondrial ROS depends on the expression of uncoupling protein-2 (UCP-2). Reactive Oxygen Species 62-65 uncoupling protein 2 Homo sapiens 95-115 29861658-8 2018 Moreover, the effect of GA on the regulation of mitochondrial ROS depends on the expression of uncoupling protein-2 (UCP-2). Reactive Oxygen Species 62-65 uncoupling protein 2 Homo sapiens 117-122 29887950-3 2018 Sepsis can trigger mitochondrial dysfunction in myocardial cells, which leads to ROS overproduction, while uncoupling protein 2 (UCP2) can reduce ROS production. Reactive Oxygen Species 146-149 uncoupling protein 2 Homo sapiens 107-127 29887950-3 2018 Sepsis can trigger mitochondrial dysfunction in myocardial cells, which leads to ROS overproduction, while uncoupling protein 2 (UCP2) can reduce ROS production. Reactive Oxygen Species 146-149 uncoupling protein 2 Homo sapiens 129-133 29849618-1 2018 Objective: To investigate the association of polymorphisms in uncoupling protein 2 (UCP2) and peroxisome proliferator-activated receptor (PPARgamma) with glucolipid metabolism in Chinese Han population. glucolipid 154-164 uncoupling protein 2 Homo sapiens 62-82 29849618-1 2018 Objective: To investigate the association of polymorphisms in uncoupling protein 2 (UCP2) and peroxisome proliferator-activated receptor (PPARgamma) with glucolipid metabolism in Chinese Han population. glucolipid 154-164 uncoupling protein 2 Homo sapiens 84-88 29547569-6 2018 Consistently, genipin, a pharmacologic inhibitor of UCP2, augmented LPS-induced damage of AC16 cells. genipin 14-21 uncoupling protein 2 Homo sapiens 52-56 29786102-6 2018 Also, patients with UCP2 -866 (rs659366) AA showed increased levels of VAF (Pc = 0.003), low levels of SAF (Pc = 0.001) and a high VAT/SAT ratio (Pc = 0.002), whereas patients with the UCP3 -55 (rs1800849) TT presented high levels of VAF (Pc = 0.002). vaf 71-74 uncoupling protein 2 Homo sapiens 20-24 29786102-6 2018 Also, patients with UCP2 -866 (rs659366) AA showed increased levels of VAF (Pc = 0.003), low levels of SAF (Pc = 0.001) and a high VAT/SAT ratio (Pc = 0.002), whereas patients with the UCP3 -55 (rs1800849) TT presented high levels of VAF (Pc = 0.002). Safflower Oil 103-106 uncoupling protein 2 Homo sapiens 20-24 29786102-6 2018 Also, patients with UCP2 -866 (rs659366) AA showed increased levels of VAF (Pc = 0.003), low levels of SAF (Pc = 0.001) and a high VAT/SAT ratio (Pc = 0.002), whereas patients with the UCP3 -55 (rs1800849) TT presented high levels of VAF (Pc = 0.002). vaf 234-237 uncoupling protein 2 Homo sapiens 20-24 29391397-12 2018 The highest concentration of BT (1 mM) increased the expression of genes involved in mitochondrial fission (PINK1, DRP1, FIS1) and physiological stress (UCP2, mTOR, HIF1alpha, PGC1alpha) as well as genes thought to be linked to cognition and behavior (CREB1, CamKinase II). Butyrates 29-31 uncoupling protein 2 Homo sapiens 153-157 28894292-8 2018 Increased expression of UCP2 not only facilitated fatty acid oxidation (increased 15-fold following surgery) but also regulated the subcutaneous adipose tissue redoxome by attenuating protein cysteine oxidation and reducing oxidative stress. Fatty Acids 50-60 uncoupling protein 2 Homo sapiens 24-28 28875237-0 2018 Uncoupling Protein 2 Inhibition Exacerbates Glucose Fluctuation-Mediated Neuronal Effects. Glucose 44-51 uncoupling protein 2 Homo sapiens 0-20 28875237-5 2018 GV also caused an increase in the glutathione/glutathione disulfide ratio and in the protein expression levels of nuclear factor E2-related factor 2 (NRF2), UCP2, NADH-ubiquinone oxidoreductase chain 1 (ND1), and mitochondrially encoded cytochrome c oxidase I (MTCO1), both mitochondrial DNA encoded subunits of the electron transport chain. gv 0-2 uncoupling protein 2 Homo sapiens 157-161 28875237-8 2018 Overall, these observations suggest that UCP2 is in the core of neuronal cell protection and/or adaptation against GV-mediated effects and that other isoforms of neuronal UCPs can be upregulated to compensate the inhibition of UCP2 activity. gv 115-117 uncoupling protein 2 Homo sapiens 41-45 28894292-8 2018 Increased expression of UCP2 not only facilitated fatty acid oxidation (increased 15-fold following surgery) but also regulated the subcutaneous adipose tissue redoxome by attenuating protein cysteine oxidation and reducing oxidative stress. Cysteine 192-200 uncoupling protein 2 Homo sapiens 24-28 30355916-3 2018 UCP2 protein in spermatozoawas quantified by Western blotting. spermatozoawas 16-30 uncoupling protein 2 Homo sapiens 0-4 30355916-10 2018 Unexpectedly, this treatment also has a positive impact on the expression of UCP2 within a certain range of supplemental H2O2, indicating the moderate mROS level possibly serves as a feedback signal to stimulate the expression of UCP2. Hydrogen Peroxide 121-125 uncoupling protein 2 Homo sapiens 77-81 30355916-11 2018 Finally, the treatment of spermatozoa by an ROS scavenger, N-acetyl-l-cysteine (NAC),decreases the level of mROS and increases the curvilinear velocity (VCL) of spermatozoa, but the UCP2 level is not affected. Reactive Oxygen Species 44-47 uncoupling protein 2 Homo sapiens 182-186 29043564-1 2017 Recent studies based on experimental animal models of stroke have suggested that uncoupling protein 2 (UCP2), an inner mitochondrial membrane protein that is thought to regulate energy metabolism and reduce reactive oxygen species generation, provides protection against reperfusion damage. Reactive Oxygen Species 207-230 uncoupling protein 2 Homo sapiens 81-101 29043564-1 2017 Recent studies based on experimental animal models of stroke have suggested that uncoupling protein 2 (UCP2), an inner mitochondrial membrane protein that is thought to regulate energy metabolism and reduce reactive oxygen species generation, provides protection against reperfusion damage. Reactive Oxygen Species 207-230 uncoupling protein 2 Homo sapiens 103-107 28546450-6 2017 In FABP4 knockout macrophages, silencing of UCP2, increased ROS levels and led to increased expression of BLT1R mRNA. Reactive Oxygen Species 60-63 uncoupling protein 2 Homo sapiens 44-48 28424210-6 2017 Compared with littermates, deletion of Ucp2 exacerbated I/R-induced AKI whereas increase of UCP2 by conjugated linoleic acid (CLA) attenuated I/R injury. Linoleic Acid 111-124 uncoupling protein 2 Homo sapiens 92-96 28424210-6 2017 Compared with littermates, deletion of Ucp2 exacerbated I/R-induced AKI whereas increase of UCP2 by conjugated linoleic acid (CLA) attenuated I/R injury. Linoleic Acids, Conjugated 126-129 uncoupling protein 2 Homo sapiens 92-96 28771482-1 2017 Uncoupling protein 2 (UCP2) is a mitochondrial membrane protein that plays a role in uncoupling electron transport from adenosine triphosphate (ATP) formation. Adenosine 120-129 uncoupling protein 2 Homo sapiens 0-20 28771482-1 2017 Uncoupling protein 2 (UCP2) is a mitochondrial membrane protein that plays a role in uncoupling electron transport from adenosine triphosphate (ATP) formation. Adenosine 120-129 uncoupling protein 2 Homo sapiens 22-26 28771482-1 2017 Uncoupling protein 2 (UCP2) is a mitochondrial membrane protein that plays a role in uncoupling electron transport from adenosine triphosphate (ATP) formation. Adenosine Triphosphate 144-147 uncoupling protein 2 Homo sapiens 0-20 28771482-1 2017 Uncoupling protein 2 (UCP2) is a mitochondrial membrane protein that plays a role in uncoupling electron transport from adenosine triphosphate (ATP) formation. Adenosine Triphosphate 144-147 uncoupling protein 2 Homo sapiens 22-26 29254145-0 2017 Bile acid and cigarette smoke enhance the aggressive phenotype of esophageal adenocarcinoma cells by downregulation of the mitochondrial uncoupling protein-2. Bile Acids and Salts 0-9 uncoupling protein 2 Homo sapiens 123-157 29254145-9 2017 Furthermore, over-expression of UCP2 abrogated DCA and CSC-mediated increases in lactate and ATP production in EACC. Deoxycholic Acid 47-50 uncoupling protein 2 Homo sapiens 32-36 29254145-9 2017 Furthermore, over-expression of UCP2 abrogated DCA and CSC-mediated increases in lactate and ATP production in EACC. Lactic Acid 81-88 uncoupling protein 2 Homo sapiens 32-36 29254145-9 2017 Furthermore, over-expression of UCP2 abrogated DCA and CSC-mediated increases in lactate and ATP production in EACC. Adenosine Triphosphate 93-96 uncoupling protein 2 Homo sapiens 32-36 28574619-1 2017 Uncoupling protein 2 (UCP2), whose physiological role is to decrease mitochondrial membrane potential and reactive oxygen species (ROS) production, is often overexpressed in human cancers. Reactive Oxygen Species 106-129 uncoupling protein 2 Homo sapiens 0-20 28574619-1 2017 Uncoupling protein 2 (UCP2), whose physiological role is to decrease mitochondrial membrane potential and reactive oxygen species (ROS) production, is often overexpressed in human cancers. Reactive Oxygen Species 106-129 uncoupling protein 2 Homo sapiens 22-26 28574619-1 2017 Uncoupling protein 2 (UCP2), whose physiological role is to decrease mitochondrial membrane potential and reactive oxygen species (ROS) production, is often overexpressed in human cancers. Reactive Oxygen Species 131-134 uncoupling protein 2 Homo sapiens 0-20 28574619-1 2017 Uncoupling protein 2 (UCP2), whose physiological role is to decrease mitochondrial membrane potential and reactive oxygen species (ROS) production, is often overexpressed in human cancers. Reactive Oxygen Species 131-134 uncoupling protein 2 Homo sapiens 22-26 28574619-4 2017 Based on a widely used skin cell transformation model, our data demonstrated that UCP2 differentially regulated ROS. Reactive Oxygen Species 112-115 uncoupling protein 2 Homo sapiens 82-86 28574619-5 2017 UCP2 upregulation decreased superoxide whereas it increased hydrogen peroxide production with concomitant increase in the expression and activity of manganese superoxide dismutase (MnSOD), the primary mitochondrial antioxidant enzyme. Superoxides 28-38 uncoupling protein 2 Homo sapiens 0-4 28574619-5 2017 UCP2 upregulation decreased superoxide whereas it increased hydrogen peroxide production with concomitant increase in the expression and activity of manganese superoxide dismutase (MnSOD), the primary mitochondrial antioxidant enzyme. Hydrogen Peroxide 60-77 uncoupling protein 2 Homo sapiens 0-4 28574619-6 2017 Furthermore, hydrogen peroxide was responsible for induction of lipid peroxidation, and PLCgamma-1 activation in UCP2 overexpressed cells. Hydrogen Peroxide 13-30 uncoupling protein 2 Homo sapiens 113-117 28574619-9 2017 Taken together, our data suggest that (i) UCP2 is an important regulator of mitochondrial redox status and lipid signaling; (ii) hydrogen peroxide might mediate UCP2"s tumor promoting activity; and (iii) pharmacological disruption of PLCgamma-1 and/or hydrogen peroxide may have clinical utility for UCP2 overexpressed cancers. Hydrogen Peroxide 129-146 uncoupling protein 2 Homo sapiens 161-165 28574619-9 2017 Taken together, our data suggest that (i) UCP2 is an important regulator of mitochondrial redox status and lipid signaling; (ii) hydrogen peroxide might mediate UCP2"s tumor promoting activity; and (iii) pharmacological disruption of PLCgamma-1 and/or hydrogen peroxide may have clinical utility for UCP2 overexpressed cancers. Hydrogen Peroxide 129-146 uncoupling protein 2 Homo sapiens 161-165 28574619-9 2017 Taken together, our data suggest that (i) UCP2 is an important regulator of mitochondrial redox status and lipid signaling; (ii) hydrogen peroxide might mediate UCP2"s tumor promoting activity; and (iii) pharmacological disruption of PLCgamma-1 and/or hydrogen peroxide may have clinical utility for UCP2 overexpressed cancers. Hydrogen Peroxide 252-269 uncoupling protein 2 Homo sapiens 42-46 28574619-9 2017 Taken together, our data suggest that (i) UCP2 is an important regulator of mitochondrial redox status and lipid signaling; (ii) hydrogen peroxide might mediate UCP2"s tumor promoting activity; and (iii) pharmacological disruption of PLCgamma-1 and/or hydrogen peroxide may have clinical utility for UCP2 overexpressed cancers. Hydrogen Peroxide 252-269 uncoupling protein 2 Homo sapiens 161-165 28574619-9 2017 Taken together, our data suggest that (i) UCP2 is an important regulator of mitochondrial redox status and lipid signaling; (ii) hydrogen peroxide might mediate UCP2"s tumor promoting activity; and (iii) pharmacological disruption of PLCgamma-1 and/or hydrogen peroxide may have clinical utility for UCP2 overexpressed cancers. Hydrogen Peroxide 252-269 uncoupling protein 2 Homo sapiens 161-165 28317281-4 2017 The treatment of the cells with oleic acid increased reactive oxygen species (ROS) generation and expression of tumor necrosis factor alpha (TNF-alpha), decreased expression of uncoupling protein 2, and decreased mitochondrial content and markers of biogenesis. Oleic Acid 32-42 uncoupling protein 2 Homo sapiens 177-197 28317281-7 2017 Most polyphenols also prevented the decrease in uncoupling protein 2. Polyphenols 5-16 uncoupling protein 2 Homo sapiens 48-68 28737710-5 2017 Uncoupling protein 2 (UCP2) regulates ATP (Adenosine triphosphate) and reactive oxygen species production by affecting the mitochondrial respiratory chain, which may play a protective role in CIR. Adenosine Triphosphate 38-41 uncoupling protein 2 Homo sapiens 0-20 28737710-5 2017 Uncoupling protein 2 (UCP2) regulates ATP (Adenosine triphosphate) and reactive oxygen species production by affecting the mitochondrial respiratory chain, which may play a protective role in CIR. Adenosine Triphosphate 38-41 uncoupling protein 2 Homo sapiens 22-26 28737710-5 2017 Uncoupling protein 2 (UCP2) regulates ATP (Adenosine triphosphate) and reactive oxygen species production by affecting the mitochondrial respiratory chain, which may play a protective role in CIR. Adenosine Triphosphate 43-65 uncoupling protein 2 Homo sapiens 0-20 28737710-5 2017 Uncoupling protein 2 (UCP2) regulates ATP (Adenosine triphosphate) and reactive oxygen species production by affecting the mitochondrial respiratory chain, which may play a protective role in CIR. Adenosine Triphosphate 43-65 uncoupling protein 2 Homo sapiens 22-26 28737710-5 2017 Uncoupling protein 2 (UCP2) regulates ATP (Adenosine triphosphate) and reactive oxygen species production by affecting the mitochondrial respiratory chain, which may play a protective role in CIR. Reactive Oxygen Species 71-94 uncoupling protein 2 Homo sapiens 0-20 28737710-5 2017 Uncoupling protein 2 (UCP2) regulates ATP (Adenosine triphosphate) and reactive oxygen species production by affecting the mitochondrial respiratory chain, which may play a protective role in CIR. Reactive Oxygen Species 71-94 uncoupling protein 2 Homo sapiens 22-26 28483672-6 2017 The UCP2 inhibitor, genipin, increased ROS production with either acetoacetate or glucose by 2-fold, indicating a role for UCP2 in suppressing ROS production. Reactive Oxygen Species 39-42 uncoupling protein 2 Homo sapiens 4-8 28483672-6 2017 The UCP2 inhibitor, genipin, increased ROS production with either acetoacetate or glucose by 2-fold, indicating a role for UCP2 in suppressing ROS production. acetoacetic acid 66-78 uncoupling protein 2 Homo sapiens 4-8 28483672-6 2017 The UCP2 inhibitor, genipin, increased ROS production with either acetoacetate or glucose by 2-fold, indicating a role for UCP2 in suppressing ROS production. Glucose 82-89 uncoupling protein 2 Homo sapiens 4-8 28483672-6 2017 The UCP2 inhibitor, genipin, increased ROS production with either acetoacetate or glucose by 2-fold, indicating a role for UCP2 in suppressing ROS production. Reactive Oxygen Species 143-146 uncoupling protein 2 Homo sapiens 4-8 28483672-6 2017 The UCP2 inhibitor, genipin, increased ROS production with either acetoacetate or glucose by 2-fold, indicating a role for UCP2 in suppressing ROS production. Reactive Oxygen Species 143-146 uncoupling protein 2 Homo sapiens 123-127 28693257-7 2017 Uncoupling protein 2 (UCP2) is broadly expressed in cancer cells, and suppresses mitochondrial reactive oxygen species (ROS) production. Reactive Oxygen Species 95-118 uncoupling protein 2 Homo sapiens 0-20 28693257-7 2017 Uncoupling protein 2 (UCP2) is broadly expressed in cancer cells, and suppresses mitochondrial reactive oxygen species (ROS) production. Reactive Oxygen Species 95-118 uncoupling protein 2 Homo sapiens 22-26 28693257-7 2017 Uncoupling protein 2 (UCP2) is broadly expressed in cancer cells, and suppresses mitochondrial reactive oxygen species (ROS) production. Reactive Oxygen Species 120-123 uncoupling protein 2 Homo sapiens 0-20 28693257-7 2017 Uncoupling protein 2 (UCP2) is broadly expressed in cancer cells, and suppresses mitochondrial reactive oxygen species (ROS) production. Reactive Oxygen Species 120-123 uncoupling protein 2 Homo sapiens 22-26 28693257-8 2017 UCP2 contributes to both carcinogenesis and chemoresistance by reducing ROS. Reactive Oxygen Species 72-75 uncoupling protein 2 Homo sapiens 0-4 28693257-10 2017 The present study investigated the association between UCP2 expression and NAC effectiveness. nac 75-78 uncoupling protein 2 Homo sapiens 55-59 28693257-16 2017 UCP2 weighted score was higher in the NAC ineffective group than in the NAC effective group (P=0.038). nac 38-41 uncoupling protein 2 Homo sapiens 0-4 28693257-16 2017 UCP2 weighted score was higher in the NAC ineffective group than in the NAC effective group (P=0.038). nac 72-75 uncoupling protein 2 Homo sapiens 0-4 28693257-17 2017 Additionally, the low UCP2 expression group was more sensitive to NAC than the high UCP2 expression group (P=0.041). nac 66-69 uncoupling protein 2 Homo sapiens 22-26 28693257-18 2017 Sensitivity to cisplatin was significantly increased when UCP2 was inhibited in human uterine cervical cancer cells in vitro. Cisplatin 15-24 uncoupling protein 2 Homo sapiens 58-62 28693257-19 2017 UCP2 expression may become a predictive marker of whether NAC is effective for patients with locally advanced uterine cervical cancer, which could improve patient prognosis. nac 58-61 uncoupling protein 2 Homo sapiens 0-4 28969026-1 2017 The aim of this study is to demonstrate that improving the mitochondrial function can inhibite the loss of chondrocyte phenotype by regulating the expression of uncoupling protein 2(UCP2) and NADPH oxidase1/4(NOX1/4) to reduce the production of reactive oxygen species(ROS). Reactive Oxygen Species 245-268 uncoupling protein 2 Homo sapiens 161-181 28487946-0 2017 Ghrelin suppresses inflammation in HUVECs by inhibiting ubiquitin-mediated uncoupling protein 2 degradation. Ghrelin 0-7 uncoupling protein 2 Homo sapiens 75-95 28487946-4 2017 In this study, we demonstrate that the treatment of human umbilical vein endothelial cells (HUVECs) with ghrelin inhibits the oxidized low-density lipoprotein (oxLDL)-induced inflammatory response, In addition, treatment with ghrelin led to the accumulation of uncoupling protein 2 (UCP2) in the cells, thus decreasing reactive oxygen species (ROS) generation. Ghrelin 105-112 uncoupling protein 2 Homo sapiens 261-281 28487946-4 2017 In this study, we demonstrate that the treatment of human umbilical vein endothelial cells (HUVECs) with ghrelin inhibits the oxidized low-density lipoprotein (oxLDL)-induced inflammatory response, In addition, treatment with ghrelin led to the accumulation of uncoupling protein 2 (UCP2) in the cells, thus decreasing reactive oxygen species (ROS) generation. Ghrelin 105-112 uncoupling protein 2 Homo sapiens 283-287 28487946-4 2017 In this study, we demonstrate that the treatment of human umbilical vein endothelial cells (HUVECs) with ghrelin inhibits the oxidized low-density lipoprotein (oxLDL)-induced inflammatory response, In addition, treatment with ghrelin led to the accumulation of uncoupling protein 2 (UCP2) in the cells, thus decreasing reactive oxygen species (ROS) generation. Ghrelin 226-233 uncoupling protein 2 Homo sapiens 261-281 28487946-4 2017 In this study, we demonstrate that the treatment of human umbilical vein endothelial cells (HUVECs) with ghrelin inhibits the oxidized low-density lipoprotein (oxLDL)-induced inflammatory response, In addition, treatment with ghrelin led to the accumulation of uncoupling protein 2 (UCP2) in the cells, thus decreasing reactive oxygen species (ROS) generation. Ghrelin 226-233 uncoupling protein 2 Homo sapiens 283-287 28487946-5 2017 Moreover, the siRNA-mediated knockdown of UCP2 expression suggested that the inhibitory effects of ghrelin on the inflammatory response relied on its ability to induce the accumulation of cellular UCP2 levels. Ghrelin 99-106 uncoupling protein 2 Homo sapiens 42-46 28487946-5 2017 Moreover, the siRNA-mediated knockdown of UCP2 expression suggested that the inhibitory effects of ghrelin on the inflammatory response relied on its ability to induce the accumulation of cellular UCP2 levels. Ghrelin 99-106 uncoupling protein 2 Homo sapiens 197-201 28487946-6 2017 Further analysis indicated that the accumulation of UCP2 in the ghrelin-treated cells was due to the ability of ghrelin to inhibit the ubiquitination of UCP2 and prevent UCP2 degradation, resulting in the extended protein half-life of UCP2. Ghrelin 64-71 uncoupling protein 2 Homo sapiens 52-56 28487946-6 2017 Further analysis indicated that the accumulation of UCP2 in the ghrelin-treated cells was due to the ability of ghrelin to inhibit the ubiquitination of UCP2 and prevent UCP2 degradation, resulting in the extended protein half-life of UCP2. Ghrelin 64-71 uncoupling protein 2 Homo sapiens 153-157 28487946-6 2017 Further analysis indicated that the accumulation of UCP2 in the ghrelin-treated cells was due to the ability of ghrelin to inhibit the ubiquitination of UCP2 and prevent UCP2 degradation, resulting in the extended protein half-life of UCP2. Ghrelin 64-71 uncoupling protein 2 Homo sapiens 153-157 28487946-6 2017 Further analysis indicated that the accumulation of UCP2 in the ghrelin-treated cells was due to the ability of ghrelin to inhibit the ubiquitination of UCP2 and prevent UCP2 degradation, resulting in the extended protein half-life of UCP2. Ghrelin 64-71 uncoupling protein 2 Homo sapiens 153-157 28487946-6 2017 Further analysis indicated that the accumulation of UCP2 in the ghrelin-treated cells was due to the ability of ghrelin to inhibit the ubiquitination of UCP2 and prevent UCP2 degradation, resulting in the extended protein half-life of UCP2. Ghrelin 112-119 uncoupling protein 2 Homo sapiens 52-56 28487946-6 2017 Further analysis indicated that the accumulation of UCP2 in the ghrelin-treated cells was due to the ability of ghrelin to inhibit the ubiquitination of UCP2 and prevent UCP2 degradation, resulting in the extended protein half-life of UCP2. Ghrelin 112-119 uncoupling protein 2 Homo sapiens 153-157 28487946-6 2017 Further analysis indicated that the accumulation of UCP2 in the ghrelin-treated cells was due to the ability of ghrelin to inhibit the ubiquitination of UCP2 and prevent UCP2 degradation, resulting in the extended protein half-life of UCP2. Ghrelin 112-119 uncoupling protein 2 Homo sapiens 153-157 28487946-6 2017 Further analysis indicated that the accumulation of UCP2 in the ghrelin-treated cells was due to the ability of ghrelin to inhibit the ubiquitination of UCP2 and prevent UCP2 degradation, resulting in the extended protein half-life of UCP2. Ghrelin 112-119 uncoupling protein 2 Homo sapiens 153-157 28969026-1 2017 The aim of this study is to demonstrate that improving the mitochondrial function can inhibite the loss of chondrocyte phenotype by regulating the expression of uncoupling protein 2(UCP2) and NADPH oxidase1/4(NOX1/4) to reduce the production of reactive oxygen species(ROS). Reactive Oxygen Species 245-268 uncoupling protein 2 Homo sapiens 182-186 28969026-1 2017 The aim of this study is to demonstrate that improving the mitochondrial function can inhibite the loss of chondrocyte phenotype by regulating the expression of uncoupling protein 2(UCP2) and NADPH oxidase1/4(NOX1/4) to reduce the production of reactive oxygen species(ROS). Reactive Oxygen Species 269-272 uncoupling protein 2 Homo sapiens 161-181 28969026-1 2017 The aim of this study is to demonstrate that improving the mitochondrial function can inhibite the loss of chondrocyte phenotype by regulating the expression of uncoupling protein 2(UCP2) and NADPH oxidase1/4(NOX1/4) to reduce the production of reactive oxygen species(ROS). Reactive Oxygen Species 269-272 uncoupling protein 2 Homo sapiens 182-186 28969026-7 2017 Increasing and decreasing UCP2 and NOX1/4 expression, respectively, helps maintain the chondrocyte phenotype and improve mitochondrial functioning by reducing reactive oxygen species production. Reactive Oxygen Species 159-182 uncoupling protein 2 Homo sapiens 26-30 28089824-0 2017 Mitochondrial Uncoupling Protein 2 in human cumulus cells is associated with regulating autophagy and apoptosis, maintaining gap junction integrity and progesterone synthesis. Progesterone 152-164 uncoupling protein 2 Homo sapiens 0-34 28089824-1 2017 To explore the roles of mitochondrial Uncoupling Protein 2 (UCP2) in cumulus cells (CCs), human CCs were cultured in vitro, and the UCP2 was inhibited by treatment with Genipin, a special UCP inhibitor, or by RNA interference targeting UCP2. genipin 169-176 uncoupling protein 2 Homo sapiens 132-136 28089824-1 2017 To explore the roles of mitochondrial Uncoupling Protein 2 (UCP2) in cumulus cells (CCs), human CCs were cultured in vitro, and the UCP2 was inhibited by treatment with Genipin, a special UCP inhibitor, or by RNA interference targeting UCP2. genipin 169-176 uncoupling protein 2 Homo sapiens 132-136 28089824-3 2017 UCP2 inhibition caused a significant increase in cellular oxidative damage, which was reflected in alterations to several key parameters, including reactive oxygen species (ROS) and lipid peroxidation levels and the ratio of reduced GSH to GSSG. Reactive Oxygen Species 148-171 uncoupling protein 2 Homo sapiens 0-4 28089824-3 2017 UCP2 inhibition caused a significant increase in cellular oxidative damage, which was reflected in alterations to several key parameters, including reactive oxygen species (ROS) and lipid peroxidation levels and the ratio of reduced GSH to GSSG. Reactive Oxygen Species 173-176 uncoupling protein 2 Homo sapiens 0-4 28089824-3 2017 UCP2 inhibition caused a significant increase in cellular oxidative damage, which was reflected in alterations to several key parameters, including reactive oxygen species (ROS) and lipid peroxidation levels and the ratio of reduced GSH to GSSG. Glutathione Disulfide 233-236 uncoupling protein 2 Homo sapiens 0-4 28089824-3 2017 UCP2 inhibition caused a significant increase in cellular oxidative damage, which was reflected in alterations to several key parameters, including reactive oxygen species (ROS) and lipid peroxidation levels and the ratio of reduced GSH to GSSG. Glutathione Disulfide 240-244 uncoupling protein 2 Homo sapiens 0-4 28089824-7 2017 Our data indicated that UCP2 plays highly important roles in mediating ROS production and regulating apoptosis and autophagy, as well as maintaining gap junction integrity and progesterone synthesis, which suggests that UCP2 is involved in the regulation of follicle development and early embryo implantation and implies that it might serve as a potential biomarker for oocyte quality and competency. Reactive Oxygen Species 71-74 uncoupling protein 2 Homo sapiens 24-28 28089824-7 2017 Our data indicated that UCP2 plays highly important roles in mediating ROS production and regulating apoptosis and autophagy, as well as maintaining gap junction integrity and progesterone synthesis, which suggests that UCP2 is involved in the regulation of follicle development and early embryo implantation and implies that it might serve as a potential biomarker for oocyte quality and competency. Reactive Oxygen Species 71-74 uncoupling protein 2 Homo sapiens 220-224 28089824-7 2017 Our data indicated that UCP2 plays highly important roles in mediating ROS production and regulating apoptosis and autophagy, as well as maintaining gap junction integrity and progesterone synthesis, which suggests that UCP2 is involved in the regulation of follicle development and early embryo implantation and implies that it might serve as a potential biomarker for oocyte quality and competency. Progesterone 176-188 uncoupling protein 2 Homo sapiens 24-28 28089824-7 2017 Our data indicated that UCP2 plays highly important roles in mediating ROS production and regulating apoptosis and autophagy, as well as maintaining gap junction integrity and progesterone synthesis, which suggests that UCP2 is involved in the regulation of follicle development and early embryo implantation and implies that it might serve as a potential biomarker for oocyte quality and competency. Progesterone 176-188 uncoupling protein 2 Homo sapiens 220-224 26900134-8 2017 UCP2 activity influences glucose homeostasis by fine tuning intracellular events related to the cellular energy status, thereby controlling insulin secretion, food intake behavior and adiponectin secretion in pancreatic .- cells, brain and white adipose tissue, respectively. Glucose 25-32 uncoupling protein 2 Homo sapiens 0-4 27967291-1 2017 Context: The rarest genetic form of congenital hyperinsulinism (HI) has been associated with dominant inactivating mutations in uncoupling protein 2 (UCP2), a mitochondrial inner membrane carrier that modulates oxidation of glucose vs amino acids. Glucose 224-231 uncoupling protein 2 Homo sapiens 128-148 27967291-1 2017 Context: The rarest genetic form of congenital hyperinsulinism (HI) has been associated with dominant inactivating mutations in uncoupling protein 2 (UCP2), a mitochondrial inner membrane carrier that modulates oxidation of glucose vs amino acids. Glucose 224-231 uncoupling protein 2 Homo sapiens 150-154 27967291-5 2017 Results: Of 211 cases of diazoxide-responsive HI, we identified 5 unrelated children with UCP2 mutations (5 of 211; 2.4%). Diazoxide 25-34 uncoupling protein 2 Homo sapiens 90-94 28042952-4 2017 UCP2 suppression induced reactive oxygen species production and upregulation of the ABC transporter protein ABCG2, which leads to chemoresistance by promoting drug efflux. Reactive Oxygen Species 25-48 uncoupling protein 2 Homo sapiens 0-4 28042952-5 2017 UCP2 downregulation also altered metabolic rates as shown by elevated glucose uptake and reduced oxygen consumption. Glucose 70-77 uncoupling protein 2 Homo sapiens 0-4 28042952-5 2017 UCP2 downregulation also altered metabolic rates as shown by elevated glucose uptake and reduced oxygen consumption. Oxygen 97-103 uncoupling protein 2 Homo sapiens 0-4 27998096-2 2016 The proton transport activity of UCP1 and UCP2 requires activation by fatty acids. Fatty Acids 70-81 uncoupling protein 2 Homo sapiens 42-46 27899181-0 2017 Corrigendum to "Short chain fatty acids induce UCP2-mediated autophagy in hepatic cells" [Biochem. Fatty Acids, Volatile 16-39 uncoupling protein 2 Homo sapiens 47-51 29163755-5 2017 In contrast, UCP2 overexpression improves both hyperglycemia- and high-salt diet-induced endothelial dysfunction and ameliorates hypertensive target organ damage in SHRSP. Salts 71-75 uncoupling protein 2 Homo sapiens 13-17 29163755-6 2017 Moreover, drugs (fenofibrate and sitagliptin) and several vegetable compounds (extracts from Brassicaceae, berberine, curcumin, and capsaicin) are able to induce UCP2 expression level and to exert beneficial effects on the occurrence of vascular damage. Fenofibrate 17-28 uncoupling protein 2 Homo sapiens 162-166 29163755-6 2017 Moreover, drugs (fenofibrate and sitagliptin) and several vegetable compounds (extracts from Brassicaceae, berberine, curcumin, and capsaicin) are able to induce UCP2 expression level and to exert beneficial effects on the occurrence of vascular damage. Sitagliptin Phosphate 33-44 uncoupling protein 2 Homo sapiens 162-166 29163755-6 2017 Moreover, drugs (fenofibrate and sitagliptin) and several vegetable compounds (extracts from Brassicaceae, berberine, curcumin, and capsaicin) are able to induce UCP2 expression level and to exert beneficial effects on the occurrence of vascular damage. Berberine 107-116 uncoupling protein 2 Homo sapiens 162-166 29163755-6 2017 Moreover, drugs (fenofibrate and sitagliptin) and several vegetable compounds (extracts from Brassicaceae, berberine, curcumin, and capsaicin) are able to induce UCP2 expression level and to exert beneficial effects on the occurrence of vascular damage. Curcumin 118-126 uncoupling protein 2 Homo sapiens 162-166 29163755-6 2017 Moreover, drugs (fenofibrate and sitagliptin) and several vegetable compounds (extracts from Brassicaceae, berberine, curcumin, and capsaicin) are able to induce UCP2 expression level and to exert beneficial effects on the occurrence of vascular damage. Capsaicin 132-141 uncoupling protein 2 Homo sapiens 162-166 27989750-3 2016 Indeed, we show that UCP2 sensitizes pancreas cancer cells to the treatment with the glycolytic inhibitor 2-deoxy-D-glucose. Deoxyglucose 106-123 uncoupling protein 2 Homo sapiens 21-25 27989750-5 2016 In particular, we demonstrate that the antioxidant UCP2 induces the expression of hnRNPA2/B1, which is involved in the regulation of both GLUT1 and PKM2 mRNAs, and of lactate dehydrogenase (LDH) increasing the secretion of L-lactic acid. Lactic Acid 223-236 uncoupling protein 2 Homo sapiens 51-55 27989750-6 2016 We further demonstrate that the radical scavenger N-acetyl-L-cysteine reverts hnRNPA2/B1 and PKM2 inhibition by genipin indicating a role for reactive oxygen species in the metabolic reprogramming of cancer cells mediated by UCP2. Acetylcysteine 50-69 uncoupling protein 2 Homo sapiens 225-229 27989750-7 2016 We also observe an UCP2-dependent decrease in mtOXPHOS complex I (NADH dehydrogenase), complex IV (cytochrome c oxidase), complex V (ATPase) and in mitochondrial oxygen consumption, suggesting a role for UCP2 in the counteraction of pancreatic cancer cellular respiration. Oxygen 162-168 uncoupling protein 2 Homo sapiens 19-23 27989750-8 2016 All these results reveal novel mechanisms through which UCP2 promotes cancer cell proliferation with the concomitant metabolic shift from mtOXPHOS to the glycolytic pathway. mtoxphos 138-146 uncoupling protein 2 Homo sapiens 56-60 27417103-5 2016 High PAL levels elevated intracellular and mitochondrial superoxide generation; increased inflammation marker, acyl-coenzyme A (CoA) dehydrogenase, uncoupling protein 2 (UCP2), and superoxide dismutase 2 expression; and decreased hexokinase I and pyruvate dehydrogenase expression. Palmitic Acid 5-8 uncoupling protein 2 Homo sapiens 148-168 27491555-1 2016 The aim of this article is to solve an existing controversy over the involvement of uncoupling protein-2 in the impairment of glucose-stimulated insulin secretion induced by chronic exposure of beta-cells to palmitate. Glucose 126-133 uncoupling protein 2 Homo sapiens 84-104 27451963-15 2016 This effect appeared to occur by blocking the ability of UCP2 to dissipate energy and restrict ROS production through proton leakage. Reactive Oxygen Species 95-98 uncoupling protein 2 Homo sapiens 57-61 27417103-5 2016 High PAL levels elevated intracellular and mitochondrial superoxide generation; increased inflammation marker, acyl-coenzyme A (CoA) dehydrogenase, uncoupling protein 2 (UCP2), and superoxide dismutase 2 expression; and decreased hexokinase I and pyruvate dehydrogenase expression. Palmitic Acid 5-8 uncoupling protein 2 Homo sapiens 170-174 27562049-0 2016 [Effect of uncoupling protein 2 on high-glucose induced mitochondrial damage and apoptosis of cardiomyocytes]. Glucose 40-47 uncoupling protein 2 Homo sapiens 11-31 27562049-1 2016 OBJECTIVE: To observe the expression of uncoupling protein (UCP2) and its effect on modulation of mitochondrial function and apoptosis of cardiomyocytes exposed to high-glucose. Glucose 169-176 uncoupling protein 2 Homo sapiens 60-64 27562049-5 2016 CONCLUSION: The up-regulation of UCP2 expression in cardiomyocytes, induced by high-glucose, maybe a protective mechanism for the mitochondrial damage, and UCP2 may inhibit high-glucose induced cardiomyocytes apoptosis. Glucose 84-91 uncoupling protein 2 Homo sapiens 33-37 27562049-5 2016 CONCLUSION: The up-regulation of UCP2 expression in cardiomyocytes, induced by high-glucose, maybe a protective mechanism for the mitochondrial damage, and UCP2 may inhibit high-glucose induced cardiomyocytes apoptosis. Glucose 178-185 uncoupling protein 2 Homo sapiens 156-160 27429587-3 2016 RESULTS: Genipin inhibited the UCP2 mediated anti-oxidative proton leak significantly promoted the Cisplatin induced ROS and subsequent cell death, which was similar to that of UCP2-siRNA. Cisplatin 99-108 uncoupling protein 2 Homo sapiens 31-35 27551323-4 2016 EFA-CLA, compared to EFA, positively increased the mRNA expression of TSC2 and PTEN tumor suppressors as well as decreased the expression of NOTCH1, AGPS, GNA12, STAT3, UCP2, HIGD2A, HIF1A, PPKAR1A oncogenes. efa 0-3 uncoupling protein 2 Homo sapiens 169-173 27651753-3 2016 Negative regulators of ROS, such as mitochondrial uncoupling protein 2 (UCP2) are neuroprotective factors that decrease neuron loss in models of stroke, epilepsy, and parkinsonism. Reactive Oxygen Species 23-26 uncoupling protein 2 Homo sapiens 36-70 27651753-3 2016 Negative regulators of ROS, such as mitochondrial uncoupling protein 2 (UCP2) are neuroprotective factors that decrease neuron loss in models of stroke, epilepsy, and parkinsonism. Reactive Oxygen Species 23-26 uncoupling protein 2 Homo sapiens 72-76 27651753-5 2016 In this review article, we discuss published evidence supporting the hypothesis that UCP2 is a neuroprotective factor both through its direct effects in decreasing mitochondrial ROS and through its effects in astrocytes and microglia. Reactive Oxygen Species 178-181 uncoupling protein 2 Homo sapiens 85-89 27429587-3 2016 RESULTS: Genipin inhibited the UCP2 mediated anti-oxidative proton leak significantly promoted the Cisplatin induced ROS and subsequent cell death, which was similar to that of UCP2-siRNA. Reactive Oxygen Species 117-120 uncoupling protein 2 Homo sapiens 31-35 27429587-9 2016 Inhibition of UCP2-mediated proton leak with Genipin potentiated the cytotoxicity of Cisplatin. Cisplatin 85-94 uncoupling protein 2 Homo sapiens 14-18 27273589-0 2016 Common UCP2 variants contribute to serum urate concentrations and the risk of hyperuricemia. Uric Acid 41-46 uncoupling protein 2 Homo sapiens 7-11 27273589-2 2016 This study aimed to investigate the association between UCP2 variants and serum urate as well as hyperuricemia in a Chinese population. Uric Acid 80-85 uncoupling protein 2 Homo sapiens 56-60 27273589-9 2016 This present study identified a novel gene, UCP2, that influences the serum urate concentration and the risk of hyperuricemia, and the degree of association varies with gender and BMI levels. Uric Acid 76-81 uncoupling protein 2 Homo sapiens 44-48 26781513-5 2016 Ourin vitrostudy revealed a well-defined POD concentration of DOX below which adaptive induction of proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) -mediated mitochondrial genes, including NRF-1, MnSOD, UCP2, and COX1, concurred with negligible changes in mitochondrial superoxide and cytotoxicity. Doxorubicin 62-65 uncoupling protein 2 Homo sapiens 225-229 27271549-5 2016 Employing genetic (siRNA) and pharmacologic (Genipin) approaches, we note that antigen-induced UCP2 expression reduces glycolysis, fatty acid synthesis and production of reactive oxygen species to balance differentiation with survival of effector CD8+ T cells. Fatty Acids 131-141 uncoupling protein 2 Homo sapiens 95-99 27271549-5 2016 Employing genetic (siRNA) and pharmacologic (Genipin) approaches, we note that antigen-induced UCP2 expression reduces glycolysis, fatty acid synthesis and production of reactive oxygen species to balance differentiation with survival of effector CD8+ T cells. Reactive Oxygen Species 170-193 uncoupling protein 2 Homo sapiens 95-99 26848702-8 2016 Quantitative reverse transcription-polymerase chain reaction analyses revealed that Rhizoma Coptidis alkaloids could retard the synthesis of cholesterol by downregulating the mRNA expression of 3-hydroxy-3-methyl glutaryl coenzyme A reductase and accelerate the clearance of lipids by upregulating the low-density lipoprotein receptor, cholesterol 7alpha-hydroxylase, and uncoupling protein-2 expression. Cholesterol 141-152 uncoupling protein 2 Homo sapiens 372-392 27128320-7 2016 UCP2 silencing prevents the protective effect of the glucocorticoids on ROS production. Reactive Oxygen Species 72-75 uncoupling protein 2 Homo sapiens 0-4 27128320-8 2016 UCP2 induction also increases the oxygen consumption and the "proton leak" in microvascular endothelial cells. Oxygen 34-40 uncoupling protein 2 Homo sapiens 0-4 27128320-9 2016 Furthermore, glutamine supplementation augments the effect of glucocorticoids via further enhancing the expression of UCP2 at the translational level. Glutamine 13-22 uncoupling protein 2 Homo sapiens 118-122 27076074-3 2016 (2016) unravel a new mechanism underlying VMH-dependent regulation of systemic glucose homeostasis via uncoupling protein 2 (UCP2)-mediated control of mitochondrial dynamics and activation of glucose-excited neurons. Glucose 79-86 uncoupling protein 2 Homo sapiens 103-123 27076074-3 2016 (2016) unravel a new mechanism underlying VMH-dependent regulation of systemic glucose homeostasis via uncoupling protein 2 (UCP2)-mediated control of mitochondrial dynamics and activation of glucose-excited neurons. Glucose 79-86 uncoupling protein 2 Homo sapiens 125-129 26739488-0 2016 Altered expression of uncoupling protein 2 in GLP-1-producing cells after chronic high glucose exposure: implications for the pathogenesis of diabetes mellitus. Glucose 87-94 uncoupling protein 2 Homo sapiens 22-42 26739488-6 2016 Our results indicate that this abnormality is associated with a decrease in ATP production due to the elevated expression of mitochondrial uncoupling protein 2 (UCP2). Adenosine Triphosphate 76-79 uncoupling protein 2 Homo sapiens 125-159 26739488-6 2016 Our results indicate that this abnormality is associated with a decrease in ATP production due to the elevated expression of mitochondrial uncoupling protein 2 (UCP2). Adenosine Triphosphate 76-79 uncoupling protein 2 Homo sapiens 161-165 26739488-7 2016 Furthermore, UCP2 inhibition using small interfering RNA (siRNA) and the application of glibenclamide, an ATP-sensitive potassium (KATP(+)) channel blocker, reverse the GLP-1 secretion defect induced by chronic high-glucose treatment. Glucose 216-223 uncoupling protein 2 Homo sapiens 13-17 26771154-0 2016 Cytoprotective Effects of Oleanolic Acid in Human Umbilical Vascular Endothelial Cells is Mediated Via UCP2/ROS/Cytochrome C/AIF Pathway. Oleanolic Acid 26-40 uncoupling protein 2 Homo sapiens 103-107 26771154-0 2016 Cytoprotective Effects of Oleanolic Acid in Human Umbilical Vascular Endothelial Cells is Mediated Via UCP2/ROS/Cytochrome C/AIF Pathway. Reactive Oxygen Species 108-111 uncoupling protein 2 Homo sapiens 103-107 27186330-11 2016 In obese adolescents, UCP2 -866G/A was associated with blood pressure (p=0.025), total cholesterol level (p=0.025), LDL (p=0.024) level and HOMA IR (p<0.001) but not with dietary fat intake (p=0.386). Cholesterol 87-98 uncoupling protein 2 Homo sapiens 22-26 27669335-8 2016 DFO treatment decreased mRNA levels for genes indexing dendritic and synaptic development (i.e. BdnfVI,Camk2a,Vamp1,Psd95,Cfl1, Pfn1,Pfn2, and Gda) and mitochondrial function (i.e. Ucp2,Pink1, and Cox6a1). Deferoxamine 0-3 uncoupling protein 2 Homo sapiens 181-185 26846204-0 2016 Overexpression of uncoupling protein 2 inhibits the high glucose-induced apoptosis of human umbilical vein endothelial cells. Glucose 57-64 uncoupling protein 2 Homo sapiens 18-38 26846204-6 2016 Additionally, UCP2 knockdown induced caspase-3 activation and exaggerated high glucose (HG)-induced apoptosis of human umbilical vein endothelial cells (HUVECs). Glucose 79-86 uncoupling protein 2 Homo sapiens 14-18 26846204-10 2016 Furthermore, adenovirus-mediated UCP2 overexpression led to a significant increase in intracellular nitric oxide (NO) levels and a decrease in reactive oxygen species (ROS) generation in HUVECs. Nitric Oxide 100-112 uncoupling protein 2 Homo sapiens 33-37 26846204-10 2016 Furthermore, adenovirus-mediated UCP2 overexpression led to a significant increase in intracellular nitric oxide (NO) levels and a decrease in reactive oxygen species (ROS) generation in HUVECs. Reactive Oxygen Species 143-166 uncoupling protein 2 Homo sapiens 33-37 26846204-10 2016 Furthermore, adenovirus-mediated UCP2 overexpression led to a significant increase in intracellular nitric oxide (NO) levels and a decrease in reactive oxygen species (ROS) generation in HUVECs. Reactive Oxygen Species 168-171 uncoupling protein 2 Homo sapiens 33-37 26919426-3 2016 Here, we show that glucose load results in mitochondrial fission and reduced reactive oxygen species in VMH neurons mediated by dynamin-related peptide 1 (DRP1) under the control of uncoupling protein 2 (UCP2). Glucose 19-26 uncoupling protein 2 Homo sapiens 182-202 26919426-3 2016 Here, we show that glucose load results in mitochondrial fission and reduced reactive oxygen species in VMH neurons mediated by dynamin-related peptide 1 (DRP1) under the control of uncoupling protein 2 (UCP2). Glucose 19-26 uncoupling protein 2 Homo sapiens 204-208 26919426-3 2016 Here, we show that glucose load results in mitochondrial fission and reduced reactive oxygen species in VMH neurons mediated by dynamin-related peptide 1 (DRP1) under the control of uncoupling protein 2 (UCP2). Reactive Oxygen Species 77-100 uncoupling protein 2 Homo sapiens 182-202 26919426-3 2016 Here, we show that glucose load results in mitochondrial fission and reduced reactive oxygen species in VMH neurons mediated by dynamin-related peptide 1 (DRP1) under the control of uncoupling protein 2 (UCP2). Reactive Oxygen Species 77-100 uncoupling protein 2 Homo sapiens 204-208 26771380-2 2016 UCP2 inhibition may induce pancreatic adenocarcinoma cell death by increasing reactive oxygen species (ROS) levels. Reactive Oxygen Species 78-101 uncoupling protein 2 Homo sapiens 0-4 26771380-2 2016 UCP2 inhibition may induce pancreatic adenocarcinoma cell death by increasing reactive oxygen species (ROS) levels. Reactive Oxygen Species 103-106 uncoupling protein 2 Homo sapiens 0-4 27594970-8 2016 Through our research efforts, we discovered that two genes encoding mitochondrial proteins, one (Ndufc2) involved in OXPHOS complex I assembly and activity and the second one (UCP2) involved in clearance of mitochondrial ROS, are responsible, when dysregulated, for vascular damage in SHRSP. ros 221-224 uncoupling protein 2 Homo sapiens 176-180 26450681-0 2015 Regulatory role of estrogen-induced reactive oxygen species in the modulatory function of UCP 2 in papillary thyroid cancer cells. Reactive Oxygen Species 36-59 uncoupling protein 2 Homo sapiens 90-95 26526553-1 2015 BACKGROUND AND OBJECTIVE: Given the role of uncoupling protein 2 (UCP2) in the accumulation of fat in the hepatocytes and in the enhancement of protective mechanisms in acute ethanol intake, we hypothesised that UCP2 polymorphisms are likely to cause liver disease through their interactions with obesity and alcohol intake. Ethanol 175-182 uncoupling protein 2 Homo sapiens 44-64 26526553-1 2015 BACKGROUND AND OBJECTIVE: Given the role of uncoupling protein 2 (UCP2) in the accumulation of fat in the hepatocytes and in the enhancement of protective mechanisms in acute ethanol intake, we hypothesised that UCP2 polymorphisms are likely to cause liver disease through their interactions with obesity and alcohol intake. Ethanol 175-182 uncoupling protein 2 Homo sapiens 66-70 26526553-1 2015 BACKGROUND AND OBJECTIVE: Given the role of uncoupling protein 2 (UCP2) in the accumulation of fat in the hepatocytes and in the enhancement of protective mechanisms in acute ethanol intake, we hypothesised that UCP2 polymorphisms are likely to cause liver disease through their interactions with obesity and alcohol intake. Alcohols 309-316 uncoupling protein 2 Homo sapiens 66-70 26526553-1 2015 BACKGROUND AND OBJECTIVE: Given the role of uncoupling protein 2 (UCP2) in the accumulation of fat in the hepatocytes and in the enhancement of protective mechanisms in acute ethanol intake, we hypothesised that UCP2 polymorphisms are likely to cause liver disease through their interactions with obesity and alcohol intake. Alcohols 309-316 uncoupling protein 2 Homo sapiens 212-216 26526553-8 2015 CONCLUSION: In conclusion, our findings in 20 242 individuals suggest that UCP2 gene polymorphisms may cause liver dysfunction through the interaction with body fat rather than alcohol intake. Alcohols 177-184 uncoupling protein 2 Homo sapiens 75-79 26542482-9 2015 UCP2 requires activation by superoxide and lipid peroxidation derivatives. Superoxides 28-38 uncoupling protein 2 Homo sapiens 0-4 26542482-12 2015 UCP2 is inhibited by genipin, chromane compounds and short interfering RNAs (siRNA). Chromans 30-38 uncoupling protein 2 Homo sapiens 0-4 26173855-5 2015 The initial UCP2 down-regulation was paralleled by mitochondrial inner membrane potential (mMP) depolarization and increased mitochondrial reactive oxygen species production. Reactive Oxygen Species 139-162 uncoupling protein 2 Homo sapiens 12-16 26173855-6 2015 The key role of UCP2 in controlling mMP and reactive oxygen species production was confirmed by both pharmacological inhibition and down-regulation by RNA interference. Reactive Oxygen Species 44-67 uncoupling protein 2 Homo sapiens 16-20 26173855-7 2015 Additionally, UCP2-silenced microglia stimulated with lipopolysaccharide showed an enhanced inflammatory response, characterized by a greater production of nitric oxide and interleukin-6. Nitric Oxide 156-168 uncoupling protein 2 Homo sapiens 14-18 26123077-2 2015 Moreover, it has been reported that apoptotic cell clearance and ROS-mediated apoptosis critically depend on mitochondrial uncoupling protein-2 (UCP2). Reactive Oxygen Species 65-68 uncoupling protein 2 Homo sapiens 109-143 26123077-2 2015 Moreover, it has been reported that apoptotic cell clearance and ROS-mediated apoptosis critically depend on mitochondrial uncoupling protein-2 (UCP2). Reactive Oxygen Species 65-68 uncoupling protein 2 Homo sapiens 145-149 26210873-8 2015 Finally, the PPAR-gamma agonist pioglitazone increased the expression of PGC-1alpha (a mitochondrial biogenesis master regulator), UCP2 (a mitochondrial protein known to reduce ROS production), and cytochrome oxidase subunit COX1. Pioglitazone 32-44 uncoupling protein 2 Homo sapiens 131-135 25960046-2 2015 Uncoupling protein 2 (UCP2) may play a dual role in cancer, acting as a protective mechanism in normal cells, while its overexpression in cancer cells could confer resistance to chemotherapy and a higher survival through downregulation of ROS production. ros 239-242 uncoupling protein 2 Homo sapiens 0-20 25960046-2 2015 Uncoupling protein 2 (UCP2) may play a dual role in cancer, acting as a protective mechanism in normal cells, while its overexpression in cancer cells could confer resistance to chemotherapy and a higher survival through downregulation of ROS production. ros 239-242 uncoupling protein 2 Homo sapiens 22-26 25960046-3 2015 Thus, our aim was to check whether the inhibition of UCP2 expression and function increases oxidative stress and could render breast cancer cells more sensitive to cisplatin (CDDP) or tamoxifen (TAM). Cisplatin 164-173 uncoupling protein 2 Homo sapiens 53-57 25960046-3 2015 Thus, our aim was to check whether the inhibition of UCP2 expression and function increases oxidative stress and could render breast cancer cells more sensitive to cisplatin (CDDP) or tamoxifen (TAM). Cisplatin 175-179 uncoupling protein 2 Homo sapiens 53-57 25960046-3 2015 Thus, our aim was to check whether the inhibition of UCP2 expression and function increases oxidative stress and could render breast cancer cells more sensitive to cisplatin (CDDP) or tamoxifen (TAM). Tamoxifen 184-193 uncoupling protein 2 Homo sapiens 53-57 25960046-3 2015 Thus, our aim was to check whether the inhibition of UCP2 expression and function increases oxidative stress and could render breast cancer cells more sensitive to cisplatin (CDDP) or tamoxifen (TAM). Tamoxifen 195-198 uncoupling protein 2 Homo sapiens 53-57 25960046-6 2015 UCP2 inhibition and cytotoxic treatments produced a decrease in cell viability and clonogenicity, in addition to an increase in DeltaPsim, ROS production, apoptosis, and autophagy. ros 139-142 uncoupling protein 2 Homo sapiens 0-4 25960046-7 2015 It is important to note that CDDP decreased UCP2 protein levels, so that the greatest effects produced by the UCP2 inhibition in combination with a cytotoxic treatment, with regard to treatment alone, were observed in TAM+UCP2siRNA-treated cells. Cisplatin 29-33 uncoupling protein 2 Homo sapiens 44-48 25960046-7 2015 It is important to note that CDDP decreased UCP2 protein levels, so that the greatest effects produced by the UCP2 inhibition in combination with a cytotoxic treatment, with regard to treatment alone, were observed in TAM+UCP2siRNA-treated cells. Cisplatin 29-33 uncoupling protein 2 Homo sapiens 110-114 25960046-10 2015 In conclusion, UCP2 could be a therapeutic target in breast cancer, especially in those patients treated with tamoxifen. Tamoxifen 110-119 uncoupling protein 2 Homo sapiens 15-19 26321271-6 2015 Uncoupling protein 2 (UCP2) is a regulator of mitochondrial ROS generation and can antagonise oxidative stress-induced endothelial dysfunction. Reactive Oxygen Species 60-63 uncoupling protein 2 Homo sapiens 0-20 26321271-6 2015 Uncoupling protein 2 (UCP2) is a regulator of mitochondrial ROS generation and can antagonise oxidative stress-induced endothelial dysfunction. Reactive Oxygen Species 60-63 uncoupling protein 2 Homo sapiens 22-26 26218518-1 2015 INTRODUCTION: Uncoupling protein 2 (UCP2) reduces production of reactive oxygen species (ROS) by mitochondria. Reactive Oxygen Species 64-87 uncoupling protein 2 Homo sapiens 14-34 26218518-1 2015 INTRODUCTION: Uncoupling protein 2 (UCP2) reduces production of reactive oxygen species (ROS) by mitochondria. Reactive Oxygen Species 64-87 uncoupling protein 2 Homo sapiens 36-40 26218518-1 2015 INTRODUCTION: Uncoupling protein 2 (UCP2) reduces production of reactive oxygen species (ROS) by mitochondria. Reactive Oxygen Species 89-92 uncoupling protein 2 Homo sapiens 14-34 26218518-1 2015 INTRODUCTION: Uncoupling protein 2 (UCP2) reduces production of reactive oxygen species (ROS) by mitochondria. Reactive Oxygen Species 89-92 uncoupling protein 2 Homo sapiens 36-40 25910810-0 2015 Increased activity of mitochondrial uncoupling protein 2 improves stress resistance in cultured endothelial cells exposed in vitro to high glucose levels. Glucose 139-146 uncoupling protein 2 Homo sapiens 22-56 25910810-3 2015 We have described a functional characteristic and an antioxidative role for UCP2 in endothelial cells and isolated mitochondria and how this function is altered by long-term growth in high concentrations of glucose. Glucose 207-214 uncoupling protein 2 Homo sapiens 76-80 25910810-6 2015 More pronounced control of the respiratory rate, membrane potential, and ROS by UCP2 was observed in these mitochondria. ros 73-76 uncoupling protein 2 Homo sapiens 80-84 25910810-7 2015 A greater UCP2-mediated decrease in ROS generation indicates an improved antioxidative role for UCP2 under high glucose conditions. ros 36-39 uncoupling protein 2 Homo sapiens 10-14 25910810-7 2015 A greater UCP2-mediated decrease in ROS generation indicates an improved antioxidative role for UCP2 under high glucose conditions. ros 36-39 uncoupling protein 2 Homo sapiens 96-100 25910810-7 2015 A greater UCP2-mediated decrease in ROS generation indicates an improved antioxidative role for UCP2 under high glucose conditions. Glucose 112-119 uncoupling protein 2 Homo sapiens 10-14 25910810-7 2015 A greater UCP2-mediated decrease in ROS generation indicates an improved antioxidative role for UCP2 under high glucose conditions. Glucose 112-119 uncoupling protein 2 Homo sapiens 96-100 25910810-9 2015 UCP2 gene silencing led to elevated mitochondrial ROS formation and ICAM1 expression, especially in high glucose-cultured cells. ros 50-53 uncoupling protein 2 Homo sapiens 0-4 25910810-9 2015 UCP2 gene silencing led to elevated mitochondrial ROS formation and ICAM1 expression, especially in high glucose-cultured cells. Glucose 105-112 uncoupling protein 2 Homo sapiens 0-4 25910810-13 2015 Our results indicate that endothelial UCP2 may function as a sensor and negative regulator of mitochondrial ROS production in response to hyperglycemia. ros 108-111 uncoupling protein 2 Homo sapiens 38-42 26864036-12 2016 Hence, UCP2/K177E and UCP2/G174L produced the functional incompetence of the glycine-rich motif 1. Glycine 77-84 uncoupling protein 2 Homo sapiens 7-11 26864036-12 2016 Hence, UCP2/K177E and UCP2/G174L produced the functional incompetence of the glycine-rich motif 1. Glycine 77-84 uncoupling protein 2 Homo sapiens 22-26 26642043-0 2015 Defective Expression of the Mitochondrial-tRNA Modifying Enzyme GTPBP3 Triggers AMPK-Mediated Adaptive Responses Involving Complex I Assembly Factors, Uncoupling Protein 2, and the Mitochondrial Pyruvate Carrier. Pyruvic Acid 195-203 uncoupling protein 2 Homo sapiens 151-171 26642043-10 2015 These data are compatible with a model in which high UCP2 levels, together with a reduction in pyruvate transport due to the down-regulation of MPC, promote a shift from pyruvate to fatty acid oxidation, and to an uncoupling of glycolysis and oxidative phosphorylation. Pyruvic Acid 95-103 uncoupling protein 2 Homo sapiens 53-57 26642043-10 2015 These data are compatible with a model in which high UCP2 levels, together with a reduction in pyruvate transport due to the down-regulation of MPC, promote a shift from pyruvate to fatty acid oxidation, and to an uncoupling of glycolysis and oxidative phosphorylation. Pyruvic Acid 170-178 uncoupling protein 2 Homo sapiens 53-57 26642043-10 2015 These data are compatible with a model in which high UCP2 levels, together with a reduction in pyruvate transport due to the down-regulation of MPC, promote a shift from pyruvate to fatty acid oxidation, and to an uncoupling of glycolysis and oxidative phosphorylation. Fatty Acids 182-192 uncoupling protein 2 Homo sapiens 53-57 26276275-10 2015 UCP2-mediated electric current exhibited higher reactive oxygen species (ROS) reduction effect per unit electric current production than that of non-UCP2-mediated electric current. Reactive Oxygen Species 48-71 uncoupling protein 2 Homo sapiens 0-4 26276275-10 2015 UCP2-mediated electric current exhibited higher reactive oxygen species (ROS) reduction effect per unit electric current production than that of non-UCP2-mediated electric current. Reactive Oxygen Species 73-76 uncoupling protein 2 Homo sapiens 0-4 26349987-1 2015 AD is a common neurodegenerative disease characterized by aggregated amyloid-beta (Abeta) peptide, and oxidative stress, while uncoupling protein 2 (UCP2) is a member of the anion carrier family, predicted the existence of a protein-regulated proton leak with the main purpose of controlling mitochondrial oxidative stress, reduce the generation of superoxide anion. Superoxides 349-365 uncoupling protein 2 Homo sapiens 127-147 26349987-1 2015 AD is a common neurodegenerative disease characterized by aggregated amyloid-beta (Abeta) peptide, and oxidative stress, while uncoupling protein 2 (UCP2) is a member of the anion carrier family, predicted the existence of a protein-regulated proton leak with the main purpose of controlling mitochondrial oxidative stress, reduce the generation of superoxide anion. Superoxides 349-365 uncoupling protein 2 Homo sapiens 149-153 26349987-3 2015 Our results provide novel insight that UCP2 may protect hippocampal neurons exposed to amyloid beta protein through decreasing ROS production. ros 127-130 uncoupling protein 2 Homo sapiens 39-43 25251374-0 2015 UCP2-related mitochondrial pathway participates in oroxylin A-induced apoptosis in human colon cancer cells. 5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one 51-61 uncoupling protein 2 Homo sapiens 0-4 25351290-1 2015 BACKGROUND & AIMS: Uncoupling protein 2 - UCP2 - regulates mitochondrial lipid fluxes and reactive oxygen species production by the respiratory chain. Adenosine Monophosphate 12-15 uncoupling protein 2 Homo sapiens 23-43 25351290-1 2015 BACKGROUND & AIMS: Uncoupling protein 2 - UCP2 - regulates mitochondrial lipid fluxes and reactive oxygen species production by the respiratory chain. Adenosine Monophosphate 12-15 uncoupling protein 2 Homo sapiens 46-50 25351290-1 2015 BACKGROUND & AIMS: Uncoupling protein 2 - UCP2 - regulates mitochondrial lipid fluxes and reactive oxygen species production by the respiratory chain. Reactive Oxygen Species 94-117 uncoupling protein 2 Homo sapiens 23-43 25351290-1 2015 BACKGROUND & AIMS: Uncoupling protein 2 - UCP2 - regulates mitochondrial lipid fluxes and reactive oxygen species production by the respiratory chain. Reactive Oxygen Species 94-117 uncoupling protein 2 Homo sapiens 46-50 25351290-5 2015 The UCP2 -866 G>A polymorphism was determined by allele specific oligonucleotide probes, hepatic UCP2 mRNA levels by quantitative real-time PCR. Oligonucleotides 68-83 uncoupling protein 2 Homo sapiens 4-8 25351290-10 2015 Concerning the metabolic traits, the UCP2 A/A genotype was associated with higher total serum cholesterol levels (adjusted P = 0.03), but not with serum HDL, triglycerides or impaired fasting glucose/diabetes. Cholesterol 94-105 uncoupling protein 2 Homo sapiens 37-41 25789405-1 2015 Residing at the inner mitochondrial membrane, uncoupling protein-2 (UCP2) mediates proton transport from the intermembrane space (IMS) to the mitochondrial matrix and consequently reduces the rate of ATP synthesis in the mitochondria. Adenosine Triphosphate 200-203 uncoupling protein 2 Homo sapiens 46-66 25789405-1 2015 Residing at the inner mitochondrial membrane, uncoupling protein-2 (UCP2) mediates proton transport from the intermembrane space (IMS) to the mitochondrial matrix and consequently reduces the rate of ATP synthesis in the mitochondria. Adenosine Triphosphate 200-203 uncoupling protein 2 Homo sapiens 68-72 25789405-2 2015 The ubiquitous expression of UCP2 in humans can be attributed to the protein"s multiple physiological roles in tissues, including its involvement in protective mechanisms against oxidative stress, as well as glucose and lipid metabolisms. Glucose 208-215 uncoupling protein 2 Homo sapiens 29-33 25789405-4 2015 UCP2-mediated proton transport is activated by fatty acids and inhibited by di- and triphosphate purine nucleotides. Fatty Acids 47-58 uncoupling protein 2 Homo sapiens 0-4 25789405-4 2015 UCP2-mediated proton transport is activated by fatty acids and inhibited by di- and triphosphate purine nucleotides. di- and triphosphate purine nucleotides 76-115 uncoupling protein 2 Homo sapiens 0-4 25789405-5 2015 UCP2 also transports chloride and some other small anions. Chlorides 21-29 uncoupling protein 2 Homo sapiens 0-4 25789405-10 2015 The wild type UCP2 and its mutants were purified and reconstituted into liposomes, and their conformation and ion (proton and chloride) transport activity were studied. Chlorides 126-134 uncoupling protein 2 Homo sapiens 14-18 25789405-11 2015 TM2 Arg residues at the matrix interface of UCP2 proved to be crucial for the protein"s anion transport function, and their absence resulted in highly diminished Cl(-) transport rates. Arginine 4-7 uncoupling protein 2 Homo sapiens 44-48 25789405-12 2015 On the other hand, the two other positively charged residues of TM2, located at the UCP2-IMS interface, could participate in the salt-bridge formation in the protein and promote the interhelical tight packing in the UCP2. Salts 129-133 uncoupling protein 2 Homo sapiens 84-88 25789405-13 2015 Absence of these residues did not influence Cl(-) transport rates, but disturbed the dense packing in UCP2 and resulted in higher UCP2-mediated proton transport rates in the presence of long chain fatty acids. long chain fatty acids 186-208 uncoupling protein 2 Homo sapiens 102-106 25789405-13 2015 Absence of these residues did not influence Cl(-) transport rates, but disturbed the dense packing in UCP2 and resulted in higher UCP2-mediated proton transport rates in the presence of long chain fatty acids. long chain fatty acids 186-208 uncoupling protein 2 Homo sapiens 130-134 26082030-6 2015 To study these properties, we generated Ucp2 mutations that affected three different functions of Ucp2, namely, dissipation of the mitochondrial membrane potential, transfer of anions, and binding of purine nucleotides. Purine Nucleotides 200-218 uncoupling protein 2 Homo sapiens 40-44 26107945-0 2015 MiR-133a Is Functionally Involved in Doxorubicin-Resistance in Breast Cancer Cells MCF-7 via Its Regulation of the Expression of Uncoupling Protein 2. mir-133a 0-8 uncoupling protein 2 Homo sapiens 129-149 26107945-3 2015 In the present study we found that lower level of miR133a is accompanied by increased expression of UCP-2 in Doxorubicin-resistant breast cancer cell cline MCF-7/Dox as compared with its parental cell line MCF-7. Doxorubicin 109-120 uncoupling protein 2 Homo sapiens 100-105 26107945-3 2015 In the present study we found that lower level of miR133a is accompanied by increased expression of UCP-2 in Doxorubicin-resistant breast cancer cell cline MCF-7/Dox as compared with its parental cell line MCF-7. Doxorubicin 109-112 uncoupling protein 2 Homo sapiens 100-105 26181366-0 2015 Uncoupling protein 2 mediates resistance to gemcitabine-induced apoptosis in hepatocellular carcinoma cell lines. gemcitabine 44-55 uncoupling protein 2 Homo sapiens 0-20 26181366-3 2015 Mitochondrial uncoupling protein 2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) generation, thus mitigating oxidative stress-induced apoptosis. Reactive Oxygen Species 77-100 uncoupling protein 2 Homo sapiens 0-34 26181366-3 2015 Mitochondrial uncoupling protein 2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) generation, thus mitigating oxidative stress-induced apoptosis. Reactive Oxygen Species 77-100 uncoupling protein 2 Homo sapiens 36-40 26181366-3 2015 Mitochondrial uncoupling protein 2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) generation, thus mitigating oxidative stress-induced apoptosis. Reactive Oxygen Species 102-105 uncoupling protein 2 Homo sapiens 0-34 26181366-3 2015 Mitochondrial uncoupling protein 2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) generation, thus mitigating oxidative stress-induced apoptosis. Reactive Oxygen Species 102-105 uncoupling protein 2 Homo sapiens 36-40 26181366-5 2015 Moreover, ectopic overexpression of UCP2 in a HCC cell line with low endogenous UCP2 expression, HLE, significantly decreased mitochondrial superoxide induction by the anti-cancer drug GEM. Superoxides 140-150 uncoupling protein 2 Homo sapiens 36-40 26181366-6 2015 Conversely, UCP2 mRNA silencing by RNA interference in HCC cell lines with high endogenous UCP2 expression significantly enhanced GEM-induced mitochondrial superoxide generation and apoptosis. gemcitabine 130-133 uncoupling protein 2 Homo sapiens 12-16 26181366-6 2015 Conversely, UCP2 mRNA silencing by RNA interference in HCC cell lines with high endogenous UCP2 expression significantly enhanced GEM-induced mitochondrial superoxide generation and apoptosis. gemcitabine 130-133 uncoupling protein 2 Homo sapiens 91-95 26181366-6 2015 Conversely, UCP2 mRNA silencing by RNA interference in HCC cell lines with high endogenous UCP2 expression significantly enhanced GEM-induced mitochondrial superoxide generation and apoptosis. Superoxides 156-166 uncoupling protein 2 Homo sapiens 12-16 25976474-2 2015 In this context, the study reported here revealed for the first time that cooperativity between Apoptosis Antagonizing Transcription Factor (AATF) mRNA and miR-2909 within cellular AATF RNome ensures the regulation of mitochondrial uncoupling protein 2 (UCP2) expression in a cyclic fashion and this phenomenon is substantiated when the immune cells face high glucose threat. Glucose 360-367 uncoupling protein 2 Homo sapiens 218-252 25976474-2 2015 In this context, the study reported here revealed for the first time that cooperativity between Apoptosis Antagonizing Transcription Factor (AATF) mRNA and miR-2909 within cellular AATF RNome ensures the regulation of mitochondrial uncoupling protein 2 (UCP2) expression in a cyclic fashion and this phenomenon is substantiated when the immune cells face high glucose threat. Glucose 360-367 uncoupling protein 2 Homo sapiens 254-258 25351290-11 2015 CONCLUSIONS: UCP2 -866 A/A genotype is associated with increased hepatic UCP2 expression and reduced risk of nonalcoholic steatohepatitis, particularly in subjects with normal fasting glucose. Glucose 184-191 uncoupling protein 2 Homo sapiens 13-17 25805929-6 2015 In addition, recent discoveries established a key role of UCP2 in protecting cancer cells from an excessive production of mitochondrial superoxide ions and in the promotion of cancer cell metabolic reprogramming, including aerobic glycolysis stimulation, promotion of cancer progression. Superoxides 136-146 uncoupling protein 2 Homo sapiens 58-62 25251374-3 2015 This paper explores the mechanism how oroxylin A induce apoptosis by regulating uncoupling protein 2 (UCP2) in human colon cancer cells. 5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one 38-48 uncoupling protein 2 Homo sapiens 80-100 25251374-3 2015 This paper explores the mechanism how oroxylin A induce apoptosis by regulating uncoupling protein 2 (UCP2) in human colon cancer cells. 5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one 38-48 uncoupling protein 2 Homo sapiens 102-106 25251374-4 2015 We found that the inhibition of UCP2 by UCP2 siRNA significantly increased the sensitivity of cells to drugs, reactive oxygen species (ROS) generation and the opening of mitochondrial permeability transition pore (MPTP) of CaCo-2 cells. Reactive Oxygen Species 110-133 uncoupling protein 2 Homo sapiens 32-36 25251374-4 2015 We found that the inhibition of UCP2 by UCP2 siRNA significantly increased the sensitivity of cells to drugs, reactive oxygen species (ROS) generation and the opening of mitochondrial permeability transition pore (MPTP) of CaCo-2 cells. Reactive Oxygen Species 110-133 uncoupling protein 2 Homo sapiens 40-44 25251374-4 2015 We found that the inhibition of UCP2 by UCP2 siRNA significantly increased the sensitivity of cells to drugs, reactive oxygen species (ROS) generation and the opening of mitochondrial permeability transition pore (MPTP) of CaCo-2 cells. Reactive Oxygen Species 135-138 uncoupling protein 2 Homo sapiens 32-36 25251374-4 2015 We found that the inhibition of UCP2 by UCP2 siRNA significantly increased the sensitivity of cells to drugs, reactive oxygen species (ROS) generation and the opening of mitochondrial permeability transition pore (MPTP) of CaCo-2 cells. Reactive Oxygen Species 135-138 uncoupling protein 2 Homo sapiens 40-44 25251374-5 2015 We also found that UCP2 inhibition could lead to ROS-mediated MPTP activation. Reactive Oxygen Species 49-52 uncoupling protein 2 Homo sapiens 19-23 25251374-6 2015 Furthermore, we demonstrated that oroxylin A triggered MPTP-dependent pro-apoptotic protein release from mitochondria to matrix and then induced apoptotic cascade by inhibiting UCP2. 5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one 34-44 uncoupling protein 2 Homo sapiens 177-181 25251374-7 2015 Intriguingly, the inhibition of UCP2 by oroxylin A was able to block Bcl-2 translocation to the mitochondria, keeping MPTP at open-state. 5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one 40-50 uncoupling protein 2 Homo sapiens 32-36 25251374-8 2015 In conclusion, we have demonstrated that UCP2 plays a key role in mitochondrial apoptotic pathway; UCP2s inhibition by oroxylin A triggers the MPTP opening, and promotes the apoptosis in CaCo-2 cells. 5,7-dihydroxy-6-methoxy-2-phenylchromen-4-one 119-129 uncoupling protein 2 Homo sapiens 99-103 25407516-0 2014 N-3 polyunsaturated fatty acids decrease levels of doxorubicin-induced reactive oxygen species in cardiomyocytes -- involvement of uncoupling protein UCP2. Fatty Acids, Omega-3 0-31 uncoupling protein 2 Homo sapiens 150-154 26356408-1 2015 UCP2 plays a physiological role by regulating mitochondrial biogenesis, maintaining energy balance, ROS elimination, and regulating cellular autophagy in numerous tissues. ros 100-103 uncoupling protein 2 Homo sapiens 0-4 26356408-5 2015 The levels of ROS and Mn-SOD were markedly elevated after UCP2 inhibited Genipin. ros 14-17 uncoupling protein 2 Homo sapiens 58-62 26356408-10 2015 This study indicated that UCP2 is expressed in human cumulus cells and plays important roles on mediate ROS production, apoptotic process, and steroidogenesis, suggesting UCP2 may be involved in regulation of follicle development and oocyte maturation and quality. ros 104-107 uncoupling protein 2 Homo sapiens 26-30 25407516-0 2014 N-3 polyunsaturated fatty acids decrease levels of doxorubicin-induced reactive oxygen species in cardiomyocytes -- involvement of uncoupling protein UCP2. Doxorubicin 51-62 uncoupling protein 2 Homo sapiens 150-154 25407516-5 2014 Treatment with 100 muM EPA or 50 muM DHA for 24 h resulted in a maximal mitochondria concentration of these fatty acids and increased UCP2 expression. Eicosapentaenoic Acid 23-26 uncoupling protein 2 Homo sapiens 134-138 25407516-5 2014 Treatment with 100 muM EPA or 50 muM DHA for 24 h resulted in a maximal mitochondria concentration of these fatty acids and increased UCP2 expression. Docosahexaenoic Acids 37-40 uncoupling protein 2 Homo sapiens 134-138 25407516-6 2014 Pretreatment with 100 muM EPA or 50 muM DHA prevented the DOX-induced decrease in UCP2 mRNA and protein levels, but these effects were not seen with EPA or DHA and DOX cotreatment. Eicosapentaenoic Acid 26-29 uncoupling protein 2 Homo sapiens 82-86 25407516-6 2014 Pretreatment with 100 muM EPA or 50 muM DHA prevented the DOX-induced decrease in UCP2 mRNA and protein levels, but these effects were not seen with EPA or DHA and DOX cotreatment. Docosahexaenoic Acids 40-43 uncoupling protein 2 Homo sapiens 82-86 25407516-6 2014 Pretreatment with 100 muM EPA or 50 muM DHA prevented the DOX-induced decrease in UCP2 mRNA and protein levels, but these effects were not seen with EPA or DHA and DOX cotreatment. Doxorubicin 58-61 uncoupling protein 2 Homo sapiens 82-86 25407516-8 2014 CONCLUSION: EPA or DHA pre-treatment inhibits the DOX-induced decrease in UCP2 expression, increase in ROS production, and subsequent mitochondrial membrane potential change that contribute to the cardiotoxicity of DOX. Eicosapentaenoic Acid 12-15 uncoupling protein 2 Homo sapiens 74-78 25407516-8 2014 CONCLUSION: EPA or DHA pre-treatment inhibits the DOX-induced decrease in UCP2 expression, increase in ROS production, and subsequent mitochondrial membrane potential change that contribute to the cardiotoxicity of DOX. Docosahexaenoic Acids 19-22 uncoupling protein 2 Homo sapiens 74-78 25407516-8 2014 CONCLUSION: EPA or DHA pre-treatment inhibits the DOX-induced decrease in UCP2 expression, increase in ROS production, and subsequent mitochondrial membrane potential change that contribute to the cardiotoxicity of DOX. Doxorubicin 50-53 uncoupling protein 2 Homo sapiens 74-78 25407516-8 2014 CONCLUSION: EPA or DHA pre-treatment inhibits the DOX-induced decrease in UCP2 expression, increase in ROS production, and subsequent mitochondrial membrane potential change that contribute to the cardiotoxicity of DOX. Doxorubicin 215-218 uncoupling protein 2 Homo sapiens 74-78 25127353-0 2014 Fatty acid flippase activity of UCP2 is essential for its proton transport in mitochondria. Fatty Acids 0-10 uncoupling protein 2 Homo sapiens 32-36 27308391-1 2015 Invalidation of uncoupling protein 2 (Ucp2) increases glucose utilization and proliferation in normal cells. Glucose 54-61 uncoupling protein 2 Homo sapiens 16-36 27308391-1 2015 Invalidation of uncoupling protein 2 (Ucp2) increases glucose utilization and proliferation in normal cells. Glucose 54-61 uncoupling protein 2 Homo sapiens 38-42 25078983-8 2014 LY294002 pre-treatment significantly alleviated Ang II-induced HUVEC senescence, and partly reversed the elevation of TERT, UCP2, p-Akt, c-myc and p53 protein levels. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 0-8 uncoupling protein 2 Homo sapiens 124-128 25212780-15 2014 Control of ROS levels by PEDF was specifically linked to UCP2 regulation since PEDF-induced expression of this gene in UCP2-deficient cells was associated with a decrease in ROS production. Reactive Oxygen Species 11-14 uncoupling protein 2 Homo sapiens 57-61 25212780-15 2014 Control of ROS levels by PEDF was specifically linked to UCP2 regulation since PEDF-induced expression of this gene in UCP2-deficient cells was associated with a decrease in ROS production. Reactive Oxygen Species 11-14 uncoupling protein 2 Homo sapiens 119-123 25212780-15 2014 Control of ROS levels by PEDF was specifically linked to UCP2 regulation since PEDF-induced expression of this gene in UCP2-deficient cells was associated with a decrease in ROS production. Reactive Oxygen Species 174-177 uncoupling protein 2 Homo sapiens 57-61 25212780-15 2014 Control of ROS levels by PEDF was specifically linked to UCP2 regulation since PEDF-induced expression of this gene in UCP2-deficient cells was associated with a decrease in ROS production. Reactive Oxygen Species 174-177 uncoupling protein 2 Homo sapiens 119-123 25566617-18 2014 CONCLUSION: MCDD was found to be capable of increasing the pregnancy rate of infertility patients with PCOS, which might be associated with improving endometrial blood flow and insulin resistance, increasing the UCP2 expression, and finally improving the endometrial receptivity. mcdd 12-16 uncoupling protein 2 Homo sapiens 212-216 25256770-1 2014 Uncoupling protein 2 (UCP2) is a mitochondrial anion carrier protein, which uncouples the oxidative phosphorylation from ATP production by dissipating the proton gradient generated across the mitochondrial inner membrane. Adenosine Triphosphate 121-124 uncoupling protein 2 Homo sapiens 0-20 25256770-1 2014 Uncoupling protein 2 (UCP2) is a mitochondrial anion carrier protein, which uncouples the oxidative phosphorylation from ATP production by dissipating the proton gradient generated across the mitochondrial inner membrane. Adenosine Triphosphate 121-124 uncoupling protein 2 Homo sapiens 22-26 25256770-2 2014 UCP2 regulates not only mitochondrial ATP production, but also the generation of reactive oxygen species (ROS), considered important second-messenger signals within the cell. Adenosine Triphosphate 38-41 uncoupling protein 2 Homo sapiens 0-4 25256770-2 2014 UCP2 regulates not only mitochondrial ATP production, but also the generation of reactive oxygen species (ROS), considered important second-messenger signals within the cell. Reactive Oxygen Species 81-104 uncoupling protein 2 Homo sapiens 0-4 25256770-2 2014 UCP2 regulates not only mitochondrial ATP production, but also the generation of reactive oxygen species (ROS), considered important second-messenger signals within the cell. Reactive Oxygen Species 106-109 uncoupling protein 2 Homo sapiens 0-4 25127353-5 2014 We find, by nuclear magnetic resonance and functional mutagenesis, that UCP2 can bind FAs laterally through its peripheral site, and this intramembrane molecular recognition is essential for UCP2-catalyzed FA flipping across the membrane, which in turn is essential for proton translocation. Fatty Acids 86-89 uncoupling protein 2 Homo sapiens 72-76 25127353-5 2014 We find, by nuclear magnetic resonance and functional mutagenesis, that UCP2 can bind FAs laterally through its peripheral site, and this intramembrane molecular recognition is essential for UCP2-catalyzed FA flipping across the membrane, which in turn is essential for proton translocation. Fatty Acids 86-89 uncoupling protein 2 Homo sapiens 191-195 25127353-6 2014 The antagonist GDP binds inside the UCP2 cavity and perturbs its conformation, which can displace FA from the peripheral site as a mean of inhibiting proton currents. Guanosine Diphosphate 15-18 uncoupling protein 2 Homo sapiens 36-40 24294945-6 2014 Moreover, activation of the transcription factor peroxisome proliferator-activated receptor alpha induces expression of mitochondrial uncoupling protein 2, resulting in depletion of cellular energy (ATP) reserves. Adenosine Triphosphate 199-202 uncoupling protein 2 Homo sapiens 120-154 24965893-0 2014 The neuroprotective effect of human uncoupling protein 2 (hUCP2) requires cAMP-dependent protein kinase in a toxin model of Parkinson"s disease. Cyclic AMP 74-78 uncoupling protein 2 Homo sapiens 36-56 24965893-0 2014 The neuroprotective effect of human uncoupling protein 2 (hUCP2) requires cAMP-dependent protein kinase in a toxin model of Parkinson"s disease. Cyclic AMP 74-78 uncoupling protein 2 Homo sapiens 58-63 24965893-4 2014 We previously reported a neuroprotective effect of human uncoupling protein 2 (hUCP2) against rotenone toxicity in adult fly DA neurons. Rotenone 94-102 uncoupling protein 2 Homo sapiens 57-77 24965893-4 2014 We previously reported a neuroprotective effect of human uncoupling protein 2 (hUCP2) against rotenone toxicity in adult fly DA neurons. Rotenone 94-102 uncoupling protein 2 Homo sapiens 79-84 24965893-6 2014 In primary DA neurons, rotenone-induced mitochondrial fragmentation and lethality is attenuated as the result of hucp2 expression. Rotenone 23-31 uncoupling protein 2 Homo sapiens 113-118 24965893-7 2014 To test the idea that the neuroprotective mechanism of hUCP2 involves modulation of mitochondrial dynamics, we detect preserved mitochondrial network, mobility and fusion events in hucp2 expressing DA neurons exposed to rotenone. Rotenone 220-228 uncoupling protein 2 Homo sapiens 55-60 24965893-7 2014 To test the idea that the neuroprotective mechanism of hUCP2 involves modulation of mitochondrial dynamics, we detect preserved mitochondrial network, mobility and fusion events in hucp2 expressing DA neurons exposed to rotenone. Rotenone 220-228 uncoupling protein 2 Homo sapiens 181-186 24965893-8 2014 hucp2 expression also increases intracellular cAMP levels. Cyclic AMP 46-50 uncoupling protein 2 Homo sapiens 0-5 24965893-9 2014 Thus, we hypothesize that cAMP-dependent protein kinase (PKA) might be an effector that mediates hUCP2-associated neuroprotection against rotenone. Cyclic AMP 26-30 uncoupling protein 2 Homo sapiens 97-102 24965893-9 2014 Thus, we hypothesize that cAMP-dependent protein kinase (PKA) might be an effector that mediates hUCP2-associated neuroprotection against rotenone. Rotenone 138-146 uncoupling protein 2 Homo sapiens 97-102 24965893-10 2014 Indeed, PKA inhibitors block preserved mitochondrial integrity, movement and cell survival in hucp2 expressing DA neurons exposed to rotenone. Rotenone 133-141 uncoupling protein 2 Homo sapiens 94-99 24965893-11 2014 Taken together, we present strong evidence identifying a hUCP2-PKA axis that controls mitochondrial dynamics and survival in DA neurons exposed to rotenone implicating a novel therapeutic strategy in modifying the progression of PD pathogenesis. Rotenone 147-155 uncoupling protein 2 Homo sapiens 57-62 24833471-0 2014 Ablation of uncoupling protein 2 exacerbates salt-induced cardiovascular and renal remodeling associated with enhanced oxidative stress. Salts 45-49 uncoupling protein 2 Homo sapiens 12-32 24642125-8 2014 Tyrosine 458 in the RNA-binding protein was found to be required for suppression of UCP2 mRNA binding by Src phosphorylation. Tyrosine 0-8 uncoupling protein 2 Homo sapiens 84-88 24642125-10 2014 Consistent with the known effects of UCP2 to suppress generation of reactive oxygen species, Ang1 limited ROS production in endothelium stimulated with tumour necrosis factor-alpha. Reactive Oxygen Species 68-91 uncoupling protein 2 Homo sapiens 37-41 24642125-13 2014 The ability to rapidly upregulate UCP2 protein expression may be important in protecting endothelial cells from excessive generation of potentially damaging reactive oxygen species. Reactive Oxygen Species 157-180 uncoupling protein 2 Homo sapiens 34-38 24806964-5 2014 We demonstrate that knockdown of either of two proteins that are essential for mitochondrial Ca(2+) uptake, the mitochondrial calcium uniporter (MCU) or uncoupling protein 2 (UCP2), results in decelerated STIM1 oligomerization and impaired SOCE following cell stimulation with an inositol-1,4,5-trisphosphate (IP3)-generating agonist. Inositol 1,4,5-Trisphosphate 280-308 uncoupling protein 2 Homo sapiens 153-173 24806964-5 2014 We demonstrate that knockdown of either of two proteins that are essential for mitochondrial Ca(2+) uptake, the mitochondrial calcium uniporter (MCU) or uncoupling protein 2 (UCP2), results in decelerated STIM1 oligomerization and impaired SOCE following cell stimulation with an inositol-1,4,5-trisphosphate (IP3)-generating agonist. Inositol 1,4,5-Trisphosphate 280-308 uncoupling protein 2 Homo sapiens 175-179 24806964-5 2014 We demonstrate that knockdown of either of two proteins that are essential for mitochondrial Ca(2+) uptake, the mitochondrial calcium uniporter (MCU) or uncoupling protein 2 (UCP2), results in decelerated STIM1 oligomerization and impaired SOCE following cell stimulation with an inositol-1,4,5-trisphosphate (IP3)-generating agonist. Inositol 1,4,5-Trisphosphate 310-313 uncoupling protein 2 Homo sapiens 153-173 24806964-5 2014 We demonstrate that knockdown of either of two proteins that are essential for mitochondrial Ca(2+) uptake, the mitochondrial calcium uniporter (MCU) or uncoupling protein 2 (UCP2), results in decelerated STIM1 oligomerization and impaired SOCE following cell stimulation with an inositol-1,4,5-trisphosphate (IP3)-generating agonist. Inositol 1,4,5-Trisphosphate 310-313 uncoupling protein 2 Homo sapiens 175-179 25077985-1 2014 BACKGROUND: It has been reported that increased expression of UCP-2 in the vasculature may prevent the development of atherosclerosis in patients with increased production of reactive oxygen species, as in the diabetes, obesity or hypertension. Reactive Oxygen Species 175-198 uncoupling protein 2 Homo sapiens 62-67 25909061-4 2014 A 45 bp I/D polymorphism in hUCP2 gene was genotyped by polymerase chain reaction (PCR) amplification and agarose gel electrophoresis method. Sepharose 106-113 uncoupling protein 2 Homo sapiens 28-33 24251413-8 2014 Since oxidative stress and autophagic markers were selectively increased in some of the PD patients, we hypothesize that UCP2 expression is upregulated in response to elevated reactive oxygen species generation in affected mutation carriers and that UCP2 mRNA levels might, therefore, serve as markers of disease status in LRRK2-associated PD. Reactive Oxygen Species 176-199 uncoupling protein 2 Homo sapiens 121-125 24486445-6 2014 The defence mechanism to counteract the excess of ROS production was by the upregulation of Ucp2, Ucp3 and MnSod gene expression. Reactive Oxygen Species 50-53 uncoupling protein 2 Homo sapiens 92-96 24486445-8 2014 In myocytes co-incubated with DHA and EGCG, ROS levels and the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) ratio were similar to untreated myocytes and the decrease of oxygen consumption, higher mitochondrial mass and the overexpression of Ucp2 and Ucp3 genes were similar to the DHA-treated cells with also a higher amount of mitochondrial deoxyribonucleic acid (DNA), and reduced Drp1 and Fiss1 gene expression levels. Docosahexaenoic Acids 30-33 uncoupling protein 2 Homo sapiens 253-257 24352213-5 2014 Telmisartan also upregulated the expression of PPAR-delta target genes related to fatty acid oxidation; that is, heart type-fatty acid-binding protein and uncoupling protein-2. Fatty Acids 82-92 uncoupling protein 2 Homo sapiens 155-175 24395786-7 2014 UCP2 reconstituted in lipid vesicles catalyzed the exchange of malate, oxaloacetate, and aspartate for phosphate plus a proton from opposite sides of the membrane. Aspartic Acid 89-98 uncoupling protein 2 Homo sapiens 0-4 24591154-8 2014 Western blots of P5 cells showed that Tempol and Tempol-H upregulated expression of mitochondrial uncoupling protein-2 (UCP-2). tempol 49-55 uncoupling protein 2 Homo sapiens 84-118 24591154-8 2014 Western blots of P5 cells showed that Tempol and Tempol-H upregulated expression of mitochondrial uncoupling protein-2 (UCP-2). tempol 49-55 uncoupling protein 2 Homo sapiens 120-125 24705155-7 2014 Crotonaldehyde exposure increased TRPV1 and NADPH oxidase levels, promoted apoptosis, mitochondrial injury (decreased aconitase activity, PGC-1alpha and UCP-2) as well as production of ROS and 8-OHdG. 2-butenal 0-14 uncoupling protein 2 Homo sapiens 153-158 24448391-7 2014 Both pyridine derivatives induced thermogenesis, although trigonelline presumably promoted proton leaks, while NMP increased the concentration of the uncoupling protein-2. pyridine 5-13 uncoupling protein 2 Homo sapiens 150-170 24307565-5 2014 Inhibition of mitochondrial uncoupling protein 2 (UCP2) using a chemical inhibitor or siRNA abrogates the prosurvival effects of LIF, indicating a critical role for UCP2 in modulation of mitochondrial ROS production in survival following ROS exposure. Reactive Oxygen Species 201-204 uncoupling protein 2 Homo sapiens 14-48 24307565-5 2014 Inhibition of mitochondrial uncoupling protein 2 (UCP2) using a chemical inhibitor or siRNA abrogates the prosurvival effects of LIF, indicating a critical role for UCP2 in modulation of mitochondrial ROS production in survival following ROS exposure. Reactive Oxygen Species 201-204 uncoupling protein 2 Homo sapiens 50-54 24307565-5 2014 Inhibition of mitochondrial uncoupling protein 2 (UCP2) using a chemical inhibitor or siRNA abrogates the prosurvival effects of LIF, indicating a critical role for UCP2 in modulation of mitochondrial ROS production in survival following ROS exposure. Reactive Oxygen Species 201-204 uncoupling protein 2 Homo sapiens 165-169 24307565-5 2014 Inhibition of mitochondrial uncoupling protein 2 (UCP2) using a chemical inhibitor or siRNA abrogates the prosurvival effects of LIF, indicating a critical role for UCP2 in modulation of mitochondrial ROS production in survival following ROS exposure. Reactive Oxygen Species 238-241 uncoupling protein 2 Homo sapiens 14-48 24307565-5 2014 Inhibition of mitochondrial uncoupling protein 2 (UCP2) using a chemical inhibitor or siRNA abrogates the prosurvival effects of LIF, indicating a critical role for UCP2 in modulation of mitochondrial ROS production in survival following ROS exposure. Reactive Oxygen Species 238-241 uncoupling protein 2 Homo sapiens 50-54 24307565-5 2014 Inhibition of mitochondrial uncoupling protein 2 (UCP2) using a chemical inhibitor or siRNA abrogates the prosurvival effects of LIF, indicating a critical role for UCP2 in modulation of mitochondrial ROS production in survival following ROS exposure. Reactive Oxygen Species 238-241 uncoupling protein 2 Homo sapiens 165-169 24307565-7 2014 Although treatment with LIF alone did not increase UCP2 protein, a combination of LIF treatment and ROS stress led to increased UCP2 protein levels. Reactive Oxygen Species 100-103 uncoupling protein 2 Homo sapiens 128-132 24307565-8 2014 We conclude that LIF protects astrocytes from ROS-induced death by increasing UCP2 mRNA, allowing cells to respond to ROS stress by rapidly producing UCP2 protein that ultimately decreases endogenous mitochondrial ROS production. Reactive Oxygen Species 46-49 uncoupling protein 2 Homo sapiens 78-82 24395786-7 2014 UCP2 reconstituted in lipid vesicles catalyzed the exchange of malate, oxaloacetate, and aspartate for phosphate plus a proton from opposite sides of the membrane. Phosphates 103-112 uncoupling protein 2 Homo sapiens 0-4 24395786-8 2014 The higher levels of citric acid cycle intermediates found in the mitochondria of siUCP2-HepG2 cells compared with those found in wild-type cells in addition to the transport data indicate that, by exporting C4 compounds out of mitochondria, UCP2 limits the oxidation of acetyl-CoA-producing substrates such as glucose and enhances glutaminolysis, preventing the mitochondrial accumulation of C4 metabolites derived from glutamine. Citric Acid 21-32 uncoupling protein 2 Homo sapiens 84-88 24395786-8 2014 The higher levels of citric acid cycle intermediates found in the mitochondria of siUCP2-HepG2 cells compared with those found in wild-type cells in addition to the transport data indicate that, by exporting C4 compounds out of mitochondria, UCP2 limits the oxidation of acetyl-CoA-producing substrates such as glucose and enhances glutaminolysis, preventing the mitochondrial accumulation of C4 metabolites derived from glutamine. Acetyl Coenzyme A 271-281 uncoupling protein 2 Homo sapiens 84-88 24395786-8 2014 The higher levels of citric acid cycle intermediates found in the mitochondria of siUCP2-HepG2 cells compared with those found in wild-type cells in addition to the transport data indicate that, by exporting C4 compounds out of mitochondria, UCP2 limits the oxidation of acetyl-CoA-producing substrates such as glucose and enhances glutaminolysis, preventing the mitochondrial accumulation of C4 metabolites derived from glutamine. Glucose 311-318 uncoupling protein 2 Homo sapiens 84-88 24584700-0 2014 Inhibition of high glucose-induced apoptosis by uncoupling protein 2 in human umbilical vein endothelial cells. Glucose 19-26 uncoupling protein 2 Homo sapiens 48-68 24584700-2 2014 However, uncoupling protein 2 (UCP2) can protect retinal vascular endothelial cells from damage by inhibiting the overproduction of mitochondrial ROS, although the protective mechanism involved is not completely clear. Reactive Oxygen Species 146-149 uncoupling protein 2 Homo sapiens 9-29 24584700-2 2014 However, uncoupling protein 2 (UCP2) can protect retinal vascular endothelial cells from damage by inhibiting the overproduction of mitochondrial ROS, although the protective mechanism involved is not completely clear. Reactive Oxygen Species 146-149 uncoupling protein 2 Homo sapiens 31-35 24591154-8 2014 Western blots of P5 cells showed that Tempol and Tempol-H upregulated expression of mitochondrial uncoupling protein-2 (UCP-2). tempol 38-44 uncoupling protein 2 Homo sapiens 84-118 24591154-8 2014 Western blots of P5 cells showed that Tempol and Tempol-H upregulated expression of mitochondrial uncoupling protein-2 (UCP-2). tempol 38-44 uncoupling protein 2 Homo sapiens 120-125 24395786-0 2014 UCP2 transports C4 metabolites out of mitochondria, regulating glucose and glutamine oxidation. Glucose 63-70 uncoupling protein 2 Homo sapiens 0-4 24395786-0 2014 UCP2 transports C4 metabolites out of mitochondria, regulating glucose and glutamine oxidation. Glutamine 75-84 uncoupling protein 2 Homo sapiens 0-4 24395786-5 2014 Compared with wild-type, UCP2-silenced human hepatocellular carcinoma (HepG2) cells, grown in the presence of glucose, showed a higher inner mitochondrial membrane potential and ATP:ADP ratio associated with a lower lactate release. Glucose 110-117 uncoupling protein 2 Homo sapiens 25-29 24395786-5 2014 Compared with wild-type, UCP2-silenced human hepatocellular carcinoma (HepG2) cells, grown in the presence of glucose, showed a higher inner mitochondrial membrane potential and ATP:ADP ratio associated with a lower lactate release. Adenosine Triphosphate 178-181 uncoupling protein 2 Homo sapiens 25-29 24395786-5 2014 Compared with wild-type, UCP2-silenced human hepatocellular carcinoma (HepG2) cells, grown in the presence of glucose, showed a higher inner mitochondrial membrane potential and ATP:ADP ratio associated with a lower lactate release. Adenosine Diphosphate 182-185 uncoupling protein 2 Homo sapiens 25-29 24395786-5 2014 Compared with wild-type, UCP2-silenced human hepatocellular carcinoma (HepG2) cells, grown in the presence of glucose, showed a higher inner mitochondrial membrane potential and ATP:ADP ratio associated with a lower lactate release. Lactic Acid 216-223 uncoupling protein 2 Homo sapiens 25-29 24395786-7 2014 UCP2 reconstituted in lipid vesicles catalyzed the exchange of malate, oxaloacetate, and aspartate for phosphate plus a proton from opposite sides of the membrane. malic acid 63-69 uncoupling protein 2 Homo sapiens 0-4 24395786-7 2014 UCP2 reconstituted in lipid vesicles catalyzed the exchange of malate, oxaloacetate, and aspartate for phosphate plus a proton from opposite sides of the membrane. Oxaloacetic Acid 71-83 uncoupling protein 2 Homo sapiens 0-4 24564667-3 2014 Now we review UCP2 physiological roles emphasizing its roles in pancreatic beta-cells, such as antioxidant role, possible tuning of redox homeostasis (consequently UCP2 participation in redox regulations), and fine regulation of glucose-stimulated insulin secretion (GSIS). Glucose 229-236 uncoupling protein 2 Homo sapiens 14-18 23625627-0 2014 Forkhead box protein A1 inhibits the expression of uncoupling protein 2 in hydrogen peroxide-induced A549 cell line. Hydrogen Peroxide 75-92 uncoupling protein 2 Homo sapiens 51-71 23625627-2 2014 In this study, we show that hydrogen peroxide (H2O2) treatment upregulated expression of FoxA1 and UCP2 in the A549 cell line. Hydrogen Peroxide 28-45 uncoupling protein 2 Homo sapiens 99-103 23625627-2 2014 In this study, we show that hydrogen peroxide (H2O2) treatment upregulated expression of FoxA1 and UCP2 in the A549 cell line. Hydrogen Peroxide 47-51 uncoupling protein 2 Homo sapiens 99-103 24395786-8 2014 The higher levels of citric acid cycle intermediates found in the mitochondria of siUCP2-HepG2 cells compared with those found in wild-type cells in addition to the transport data indicate that, by exporting C4 compounds out of mitochondria, UCP2 limits the oxidation of acetyl-CoA-producing substrates such as glucose and enhances glutaminolysis, preventing the mitochondrial accumulation of C4 metabolites derived from glutamine. Glutamine 421-430 uncoupling protein 2 Homo sapiens 84-88 24564667-4 2014 For example, NADPH has been firmly established as being a modulator of GSIS and since UCP2 may influence redox homeostasis, it likely affects NADPH levels. NADP 142-147 uncoupling protein 2 Homo sapiens 86-90 24142693-10 2013 UCP2 knockdown impairs proliferation at high glucose but its absence specifically impairs ligand-induced growth when glucose levels fluctuate. Glucose 45-52 uncoupling protein 2 Homo sapiens 0-4 24142693-10 2013 UCP2 knockdown impairs proliferation at high glucose but its absence specifically impairs ligand-induced growth when glucose levels fluctuate. Glucose 117-124 uncoupling protein 2 Homo sapiens 0-4 24021091-6 2013 We apply our strategy to a structure of isoform 2 of an uncoupling protein (UCP2) binding an inhibitor recently obtained in dodecylphosphocholine detergent micelles. dodecylphosphocholine 124-145 uncoupling protein 2 Homo sapiens 76-80 23819990-6 2013 Upregulation of UCP2 was critical for controlling mitochondrial membrane potential (Deltapsi) and superoxide production. Superoxides 98-108 uncoupling protein 2 Homo sapiens 16-20 23988448-14 2013 Suppression of UCP2 by siRNA enhanced the inflammasome activity stimulated by H2O2 and, conversely, overexpression of UCP2 decreased the inflammasome activation. Hydrogen Peroxide 78-82 uncoupling protein 2 Homo sapiens 15-19 23871935-0 2013 Genistein modulates oxidative stress in breast cancer cell lines according to ERalpha/ERbeta ratio: effects on mitochondrial functionality, sirtuins, uncoupling protein 2 and antioxidant enzymes. Genistein 0-9 uncoupling protein 2 Homo sapiens 150-170 23881203-1 2013 UCP2 regulates the glucagon response to fasting and starvation. Glucagon 19-27 uncoupling protein 2 Homo sapiens 0-4 23881204-1 2013 UCP2 regulates the glucagon response to fasting and starvation. Glucagon 19-27 uncoupling protein 2 Homo sapiens 0-4 23874988-6 2013 In neurons overexpressing UCP2, exposure to ethanol resulted in significantly more effective inhibition of the NMDA-induced increase in mitochondrial free Ca(2+) levels than in those without UCP2 overexpression, despite a similarly efficient increase in intracellular Ca(2+) levels irrespective of UCP2 overexpression. N-Methylaspartate 111-115 uncoupling protein 2 Homo sapiens 26-30 23874988-7 2013 Overexpression of UCP2 significantly increased the number of dead cells in a manner prevented by ethanol in neurons exposed to glutamate. Ethanol 97-104 uncoupling protein 2 Homo sapiens 18-22 23874988-0 2013 Selective inhibition by ethanol of mitochondrial calcium influx mediated by uncoupling protein-2 in relation to N-methyl-D-aspartate cytotoxicity in cultured neurons. Ethanol 24-31 uncoupling protein 2 Homo sapiens 76-96 23874988-0 2013 Selective inhibition by ethanol of mitochondrial calcium influx mediated by uncoupling protein-2 in relation to N-methyl-D-aspartate cytotoxicity in cultured neurons. Calcium 49-56 uncoupling protein 2 Homo sapiens 76-96 23874988-7 2013 Overexpression of UCP2 significantly increased the number of dead cells in a manner prevented by ethanol in neurons exposed to glutamate. Glutamic Acid 127-136 uncoupling protein 2 Homo sapiens 18-22 23874988-0 2013 Selective inhibition by ethanol of mitochondrial calcium influx mediated by uncoupling protein-2 in relation to N-methyl-D-aspartate cytotoxicity in cultured neurons. N-Methylaspartate 112-132 uncoupling protein 2 Homo sapiens 76-96 23874988-5 2013 Lentiviral overexpression of UCP2 significantly accelerated the increase by NMDA in Rhod-2 fluorescence in neurons, without affecting Fluo-3 fluorescence for intracellular Ca(2+) levels. N-Methylaspartate 76-80 uncoupling protein 2 Homo sapiens 29-33 23874988-9 2013 Decreased protein levels of GluN2B, but not GluN2A, subunit were seen in immunoprecipitates with UCP2 from neurons with brief exposure to ethanol at concentrations over 50 mM. Ethanol 138-145 uncoupling protein 2 Homo sapiens 97-101 23874988-6 2013 In neurons overexpressing UCP2, exposure to ethanol resulted in significantly more effective inhibition of the NMDA-induced increase in mitochondrial free Ca(2+) levels than in those without UCP2 overexpression, despite a similarly efficient increase in intracellular Ca(2+) levels irrespective of UCP2 overexpression. Ethanol 44-51 uncoupling protein 2 Homo sapiens 26-30 23874988-10 2013 CONCLUSIONS/SIGNIFICANCE: Ethanol could inhibit the interaction between UCP2 and NMDAR channels to prevent the mitochondrial Ca(2+) incorporation and cell death after NMDAR activation in neurons. Ethanol 26-33 uncoupling protein 2 Homo sapiens 72-76 23124112-0 2013 UCP2 inhibition triggers ROS-dependent nuclear translocation of GAPDH and autophagic cell death in pancreatic adenocarcinoma cells. Reactive Oxygen Species 25-28 uncoupling protein 2 Homo sapiens 0-4 23842222-0 2013 Mitochondrial uncoupling protein-2 in glutamate neurotoxicity. Glutamic Acid 38-47 uncoupling protein 2 Homo sapiens 0-34 23008094-9 2013 Increased ovarian UCP2 expression in response to T3 treatment in PCOS may alter pregnenolone synthesis by influencing P450scc expression, thus altering testosterone production. Pregnenolone 80-92 uncoupling protein 2 Homo sapiens 18-22 23008094-9 2013 Increased ovarian UCP2 expression in response to T3 treatment in PCOS may alter pregnenolone synthesis by influencing P450scc expression, thus altering testosterone production. Testosterone 152-164 uncoupling protein 2 Homo sapiens 18-22 23500012-11 2013 Meanwhile GW9662 abolished the UCP2 upregulation and decreased Ca2+ activity induced by PFOS. 2-chloro-5-nitrobenzanilide 10-16 uncoupling protein 2 Homo sapiens 31-35 23414455-3 2013 We and other research groups have shown previously that UCP1- and UCP2-mediated proton transport is inhibited by purine nucleotides. Purine Nucleotides 113-131 uncoupling protein 2 Homo sapiens 66-70 23124112-1 2013 Mitochondrial uncoupling protein 2 (UCP2) can moderate oxidative stress by favoring the influx of protons into the mitochondrial matrix, thus reducing electron leakage from respiratory chain and mitochondrial superoxide production. Superoxides 209-219 uncoupling protein 2 Homo sapiens 0-34 23124112-2 2013 Here, we demonstrate that UCP2 inhibition by genipin or UCP2 siRNA strongly increases reactive oxygen species (ROS) production inhibiting pancreatic adenocarcinoma cell growth. Reactive Oxygen Species 86-109 uncoupling protein 2 Homo sapiens 26-30 23124112-2 2013 Here, we demonstrate that UCP2 inhibition by genipin or UCP2 siRNA strongly increases reactive oxygen species (ROS) production inhibiting pancreatic adenocarcinoma cell growth. Reactive Oxygen Species 86-109 uncoupling protein 2 Homo sapiens 56-60 23124112-1 2013 Mitochondrial uncoupling protein 2 (UCP2) can moderate oxidative stress by favoring the influx of protons into the mitochondrial matrix, thus reducing electron leakage from respiratory chain and mitochondrial superoxide production. Superoxides 209-219 uncoupling protein 2 Homo sapiens 36-40 23072958-0 2012 Design, synthesis and pharmacological characterization of coumarin-based fluorescent analogs of excitatory amino acid transporter subtype 1 selective inhibitors, UCPH-101 and UCPH-102. coumarin 58-66 uncoupling protein 2 Homo sapiens 162-166 23124112-2 2013 Here, we demonstrate that UCP2 inhibition by genipin or UCP2 siRNA strongly increases reactive oxygen species (ROS) production inhibiting pancreatic adenocarcinoma cell growth. Reactive Oxygen Species 111-114 uncoupling protein 2 Homo sapiens 26-30 23124112-2 2013 Here, we demonstrate that UCP2 inhibition by genipin or UCP2 siRNA strongly increases reactive oxygen species (ROS) production inhibiting pancreatic adenocarcinoma cell growth. Reactive Oxygen Species 111-114 uncoupling protein 2 Homo sapiens 56-60 23124112-3 2013 We also show that UCP2 inhibition triggers ROS-dependent nuclear translocation of the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH), formation of autophagosomes, and the expression of the autophagy marker LC3-II. Reactive Oxygen Species 43-46 uncoupling protein 2 Homo sapiens 18-22 23124112-5 2013 Furthermore, we demonstrate that autophagy induced by UCP2 inhibition determines a ROS-dependent cell death, as indicated by the apoptosis decrease in the presence of the autophagy inhibitors chloroquine (CQ) or 3-methyladenine (3-MA), or the radical scavenger NAC. Reactive Oxygen Species 83-86 uncoupling protein 2 Homo sapiens 54-58 23124112-5 2013 Furthermore, we demonstrate that autophagy induced by UCP2 inhibition determines a ROS-dependent cell death, as indicated by the apoptosis decrease in the presence of the autophagy inhibitors chloroquine (CQ) or 3-methyladenine (3-MA), or the radical scavenger NAC. Chloroquine 192-203 uncoupling protein 2 Homo sapiens 54-58 23124112-5 2013 Furthermore, we demonstrate that autophagy induced by UCP2 inhibition determines a ROS-dependent cell death, as indicated by the apoptosis decrease in the presence of the autophagy inhibitors chloroquine (CQ) or 3-methyladenine (3-MA), or the radical scavenger NAC. Chloroquine 205-207 uncoupling protein 2 Homo sapiens 54-58 23124112-5 2013 Furthermore, we demonstrate that autophagy induced by UCP2 inhibition determines a ROS-dependent cell death, as indicated by the apoptosis decrease in the presence of the autophagy inhibitors chloroquine (CQ) or 3-methyladenine (3-MA), or the radical scavenger NAC. 3-methyladenine 212-227 uncoupling protein 2 Homo sapiens 54-58 23124112-5 2013 Furthermore, we demonstrate that autophagy induced by UCP2 inhibition determines a ROS-dependent cell death, as indicated by the apoptosis decrease in the presence of the autophagy inhibitors chloroquine (CQ) or 3-methyladenine (3-MA), or the radical scavenger NAC. 3-methyladenine 229-233 uncoupling protein 2 Homo sapiens 54-58 23124112-6 2013 Intriguingly, the autophagy induced by genipin is able to potentiate the autophagic cell death triggered by gemcitabine, the standard chemotherapeutic drug for pancreatic adenocarcinoma, supporting the development of an anti-cancer therapy based on UCP2 inhibition associated to standard chemotherapy. gemcitabine 108-119 uncoupling protein 2 Homo sapiens 249-253 22349573-1 2013 CONTEXT: Uncoupling protein 2 (UCP2) is involved in regulating ATP synthesis, generation of reactive oxygen species and glucose-stimulated insulin secretion in beta-cells. Adenosine Triphosphate 63-66 uncoupling protein 2 Homo sapiens 9-29 22349573-1 2013 CONTEXT: Uncoupling protein 2 (UCP2) is involved in regulating ATP synthesis, generation of reactive oxygen species and glucose-stimulated insulin secretion in beta-cells. Adenosine Triphosphate 63-66 uncoupling protein 2 Homo sapiens 31-35 22349573-1 2013 CONTEXT: Uncoupling protein 2 (UCP2) is involved in regulating ATP synthesis, generation of reactive oxygen species and glucose-stimulated insulin secretion in beta-cells. Reactive Oxygen Species 92-115 uncoupling protein 2 Homo sapiens 9-29 22349573-1 2013 CONTEXT: Uncoupling protein 2 (UCP2) is involved in regulating ATP synthesis, generation of reactive oxygen species and glucose-stimulated insulin secretion in beta-cells. Reactive Oxygen Species 92-115 uncoupling protein 2 Homo sapiens 31-35 22349573-1 2013 CONTEXT: Uncoupling protein 2 (UCP2) is involved in regulating ATP synthesis, generation of reactive oxygen species and glucose-stimulated insulin secretion in beta-cells. Glucose 120-127 uncoupling protein 2 Homo sapiens 9-29 22349573-1 2013 CONTEXT: Uncoupling protein 2 (UCP2) is involved in regulating ATP synthesis, generation of reactive oxygen species and glucose-stimulated insulin secretion in beta-cells. Glucose 120-127 uncoupling protein 2 Homo sapiens 31-35 22349573-5 2013 DESIGN: We genotyped UCP2 rs659366 in a total of 17 636 Danish individuals and established case-control studies of obese and non-obese subjects and of type 2 diabetic and glucose-tolerant subjects. Glucose 171-178 uncoupling protein 2 Homo sapiens 21-25 23275527-6 2013 In mutation-positive diazoxide-responsive probands, 42% were GLUD1, 41% were dominant KATP mutations, and 16% were in rare genes (HADH, UCP2, HNF4A, and HNF1A). Diazoxide 21-30 uncoupling protein 2 Homo sapiens 136-140 23505621-1 2013 Uncoupling proteins 2 and 3 (UCP2 and UCP3) as mitochondrial electron transporters are involved in regulation of ATP production and energy dissipation as heat. Adenosine Triphosphate 113-116 uncoupling protein 2 Homo sapiens 0-27 23505621-1 2013 Uncoupling proteins 2 and 3 (UCP2 and UCP3) as mitochondrial electron transporters are involved in regulation of ATP production and energy dissipation as heat. Adenosine Triphosphate 113-116 uncoupling protein 2 Homo sapiens 29-33 23505621-3 2013 The aim of this study was to examine the association between maximal oxygen uptake and genetic variants of the UCP2 and UCP3 genes. Oxygen 69-75 uncoupling protein 2 Homo sapiens 111-115 23069211-1 2013 Uncoupling protein-2 (UCP2) is used by cells to control reactive oxygen species (ROS) production by mitochondria. Reactive Oxygen Species 56-79 uncoupling protein 2 Homo sapiens 0-20 23069211-1 2013 Uncoupling protein-2 (UCP2) is used by cells to control reactive oxygen species (ROS) production by mitochondria. Reactive Oxygen Species 56-79 uncoupling protein 2 Homo sapiens 22-26 23069211-1 2013 Uncoupling protein-2 (UCP2) is used by cells to control reactive oxygen species (ROS) production by mitochondria. Reactive Oxygen Species 81-84 uncoupling protein 2 Homo sapiens 0-20 23069211-1 2013 Uncoupling protein-2 (UCP2) is used by cells to control reactive oxygen species (ROS) production by mitochondria. Reactive Oxygen Species 81-84 uncoupling protein 2 Homo sapiens 22-26 23069211-3 2013 UCP2 is often overexpressed in drug resistant cancer cells and therein controls cell ROS levels and limits drug toxicity. Reactive Oxygen Species 85-88 uncoupling protein 2 Homo sapiens 0-4 23069211-4 2013 With our recent observation that glutathionylation deactivates proton leak through UCP2, we decided to test if diamide, a glutathionylation catalyst, can sensitize drug resistant cells to chemotherapeutic agents. Diamide 111-118 uncoupling protein 2 Homo sapiens 83-87 24440978-1 2013 BACKGROUND: Mitochondrial uncoupling protein 2 (UCP2) uncouples electron transport from ATP production. Adenosine Triphosphate 88-91 uncoupling protein 2 Homo sapiens 12-46 24440978-1 2013 BACKGROUND: Mitochondrial uncoupling protein 2 (UCP2) uncouples electron transport from ATP production. Adenosine Triphosphate 88-91 uncoupling protein 2 Homo sapiens 48-52 23841103-5 2013 UCP2 is expressed in several tissues and acts in the negative regulation of insulin secretion by beta-cells and in fatty acid metabolism. Fatty Acids 115-125 uncoupling protein 2 Homo sapiens 0-4 23072958-0 2012 Design, synthesis and pharmacological characterization of coumarin-based fluorescent analogs of excitatory amino acid transporter subtype 1 selective inhibitors, UCPH-101 and UCPH-102. coumarin 58-66 uncoupling protein 2 Homo sapiens 175-179 23072958-4 2012 In this paper, we present the design, synthesis and pharmacological evaluation of six coumarin-based fluorescent analogs of UCPH-101/102 as subtype-selective inhibitors at EAAT1. coumarin 86-94 uncoupling protein 2 Homo sapiens 124-128 23053476-8 2012 High glucose conditions produced elevated levels of cellular Q10, increased ROS generation, increased hexokinase I, lactate dehydrogenase, acyl-CoA dehydrogenase, uncoupling protein 2 (UCP2), and superoxide dismutase 2 expression, and decreased E3-binding protein of pyruvate dehydrogenase expression. Glucose 5-12 uncoupling protein 2 Homo sapiens 163-183 23053476-8 2012 High glucose conditions produced elevated levels of cellular Q10, increased ROS generation, increased hexokinase I, lactate dehydrogenase, acyl-CoA dehydrogenase, uncoupling protein 2 (UCP2), and superoxide dismutase 2 expression, and decreased E3-binding protein of pyruvate dehydrogenase expression. Glucose 5-12 uncoupling protein 2 Homo sapiens 185-189 23151243-0 2012 Inhibition of uncoupling protein 2 with genipin exacerbates palmitate-induced hepatic steatosis. Palmitates 60-69 uncoupling protein 2 Homo sapiens 14-34 23151243-1 2012 BACKGROUND: Uncoupling protein 2 (UCP2) was reported to be involved in lipid metabolism through regulating the production of superoxide anion. Superoxides 125-141 uncoupling protein 2 Homo sapiens 12-32 23151243-1 2012 BACKGROUND: Uncoupling protein 2 (UCP2) was reported to be involved in lipid metabolism through regulating the production of superoxide anion. Superoxides 125-141 uncoupling protein 2 Homo sapiens 34-38 23151243-6 2012 Moreover, the specific inhibition of UCP2 by genipin remarkably exacerbated PA-induced hepatocytes steatosis. Palmitic Acid 76-78 uncoupling protein 2 Homo sapiens 37-41 23151243-10 2012 CONCLUSIONS: These findings suggest that UCP2 plays a protective role in PA-induced hepatocytic steatosis through ameliorating oxidative stress. Palmitic Acid 73-75 uncoupling protein 2 Homo sapiens 41-45 22847181-0 2012 Mitochondrial uncoupling protein 2 regulates the effects of paclitaxel on Stat3 activation and cellular survival in lung cancer cells. Paclitaxel 60-70 uncoupling protein 2 Homo sapiens 0-34 22847181-5 2012 Uncoupling protein 2 (UCP-2), located in the inner membrane of the mitochondria, can reduce ROS production in conditions of oxidative stress. Reactive Oxygen Species 92-95 uncoupling protein 2 Homo sapiens 0-20 22847181-5 2012 Uncoupling protein 2 (UCP-2), located in the inner membrane of the mitochondria, can reduce ROS production in conditions of oxidative stress. Reactive Oxygen Species 92-95 uncoupling protein 2 Homo sapiens 22-27 22847181-7 2012 Silencing high UCP-2 expression with small interfering RNA (siRNA) in A549 and H460 cells restored paclitaxel-induced Stat3 activation. Paclitaxel 99-109 uncoupling protein 2 Homo sapiens 15-20 22847181-12 2012 Therefore, UCP-2 modulates the ROS/Stat3 signaling pathway and response to chemotherapy treatment in lung cancer cells. Reactive Oxygen Species 31-34 uncoupling protein 2 Homo sapiens 11-16 22710994-4 2012 Increased concentrations of uncoupling protein-2 (UCP-2) also indicated a thermogenic activity of caffeine. Caffeine 98-106 uncoupling protein 2 Homo sapiens 28-48 22825002-7 2012 Moreover, 17beta-estradiol increased the expression of the peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC1alpha), adiponectin, uncoupling protein 2 (UCP2) and glucose transporter 4 (GLUT4) genes 24 h after treatment, whereas the ER-alpha/beta non-specific antagonist, ICI 182.780 (10 microM), and the AMPK antagonist compound C (20 microM) reversed the estrogen-induced increase in the expression of these genes. Estradiol 10-26 uncoupling protein 2 Homo sapiens 153-173 22825002-7 2012 Moreover, 17beta-estradiol increased the expression of the peroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC1alpha), adiponectin, uncoupling protein 2 (UCP2) and glucose transporter 4 (GLUT4) genes 24 h after treatment, whereas the ER-alpha/beta non-specific antagonist, ICI 182.780 (10 microM), and the AMPK antagonist compound C (20 microM) reversed the estrogen-induced increase in the expression of these genes. Estradiol 10-26 uncoupling protein 2 Homo sapiens 175-179 22705884-0 2012 Role of mitochondrial uncoupling protein 2 in cancer cell resistance to gemcitabine. gemcitabine 72-83 uncoupling protein 2 Homo sapiens 8-42 22705884-2 2012 Mitochondrial uncoupling protein 2 (UCP2) can mitigate oxidative stress by increasing the influx of protons into the mitochondrial matrix and reducing electron leakage and mitochondrial superoxide generation. Superoxides 186-196 uncoupling protein 2 Homo sapiens 0-34 22705884-2 2012 Mitochondrial uncoupling protein 2 (UCP2) can mitigate oxidative stress by increasing the influx of protons into the mitochondrial matrix and reducing electron leakage and mitochondrial superoxide generation. Superoxides 186-196 uncoupling protein 2 Homo sapiens 36-40 22705884-3 2012 Here, we demonstrate that chemical uncouplers or UCP2 over-expression strongly decrease mitochondrial superoxide induction by the anticancer drug gemcitabine (GEM) and protect cancer cells from GEM-induced apoptosis. Superoxides 102-112 uncoupling protein 2 Homo sapiens 49-53 22705884-3 2012 Here, we demonstrate that chemical uncouplers or UCP2 over-expression strongly decrease mitochondrial superoxide induction by the anticancer drug gemcitabine (GEM) and protect cancer cells from GEM-induced apoptosis. gemcitabine 146-157 uncoupling protein 2 Homo sapiens 49-53 22705884-3 2012 Here, we demonstrate that chemical uncouplers or UCP2 over-expression strongly decrease mitochondrial superoxide induction by the anticancer drug gemcitabine (GEM) and protect cancer cells from GEM-induced apoptosis. gemcitabine 159-162 uncoupling protein 2 Homo sapiens 49-53 22705884-6 2012 Conversely, UCP2 inhibition by genipin or UCP2 mRNA silencing strongly enhances GEM-induced mitochondrial superoxide generation and apoptosis, synergistically inhibiting cancer cell proliferation. gemcitabine 80-83 uncoupling protein 2 Homo sapiens 12-16 22705884-6 2012 Conversely, UCP2 inhibition by genipin or UCP2 mRNA silencing strongly enhances GEM-induced mitochondrial superoxide generation and apoptosis, synergistically inhibiting cancer cell proliferation. gemcitabine 80-83 uncoupling protein 2 Homo sapiens 42-46 22705884-6 2012 Conversely, UCP2 inhibition by genipin or UCP2 mRNA silencing strongly enhances GEM-induced mitochondrial superoxide generation and apoptosis, synergistically inhibiting cancer cell proliferation. Superoxides 106-116 uncoupling protein 2 Homo sapiens 12-16 22705884-6 2012 Conversely, UCP2 inhibition by genipin or UCP2 mRNA silencing strongly enhances GEM-induced mitochondrial superoxide generation and apoptosis, synergistically inhibiting cancer cell proliferation. Superoxides 106-116 uncoupling protein 2 Homo sapiens 42-46 22710994-4 2012 Increased concentrations of uncoupling protein-2 (UCP-2) also indicated a thermogenic activity of caffeine. Caffeine 98-106 uncoupling protein 2 Homo sapiens 50-55 22490607-2 2012 In this study, we attempted to demonstrate a pivotal role of mitochondrial uncoupling protein-2 (UCP2) as a determinant of the NMDA neurotoxicity by using acquired NMDAR channels artificially orchestrated in HEK293 cells. N-Methylaspartate 127-131 uncoupling protein 2 Homo sapiens 61-95 23181133-8 2012 Moreover, hUCP2 overexpression inhibited the production of superoxide and increased the bioavailability of nitric oxide (NO). Superoxides 59-69 uncoupling protein 2 Homo sapiens 10-15 23181133-8 2012 Moreover, hUCP2 overexpression inhibited the production of superoxide and increased the bioavailability of nitric oxide (NO). Nitric Oxide 107-119 uncoupling protein 2 Homo sapiens 10-15 22490607-2 2012 In this study, we attempted to demonstrate a pivotal role of mitochondrial uncoupling protein-2 (UCP2) as a determinant of the NMDA neurotoxicity by using acquired NMDAR channels artificially orchestrated in HEK293 cells. N-Methylaspartate 127-131 uncoupling protein 2 Homo sapiens 97-101 22490607-4 2012 In cells with acquired NMDAR channels, exposure to either NMDA or the calcium ionophore A23187 similarly led to a significant increase in cytosolic Ca(2+) levels determined by Fluo-3 imaging irrespective of the overexpression of UCP2. Calcium 70-77 uncoupling protein 2 Homo sapiens 229-233 22490607-4 2012 In cells with acquired NMDAR channels, exposure to either NMDA or the calcium ionophore A23187 similarly led to a significant increase in cytosolic Ca(2+) levels determined by Fluo-3 imaging irrespective of the overexpression of UCP2. Calcimycin 88-94 uncoupling protein 2 Homo sapiens 229-233 22490607-5 2012 By contrast, NMDA, but not A23187, was significantly more effective in increasing mitochondrial Ca(2+) levels determined by Rhod-2 fluorescence imaging in cells transfected with NMDAR subunit and UCP2 expression vectors than in those without UCP2 overexpression. N-Methylaspartate 13-17 uncoupling protein 2 Homo sapiens 196-200 22490607-5 2012 By contrast, NMDA, but not A23187, was significantly more effective in increasing mitochondrial Ca(2+) levels determined by Rhod-2 fluorescence imaging in cells transfected with NMDAR subunit and UCP2 expression vectors than in those without UCP2 overexpression. N-Methylaspartate 13-17 uncoupling protein 2 Homo sapiens 242-246 22490607-6 2012 Overexpression of UCP2 significantly increased the number of cells stained with propidium iodide in cultures with acquired NMDAR channels, but failed to significantly affect that in cells exposed to A23187. Propidium 80-96 uncoupling protein 2 Homo sapiens 18-22 22807616-1 2012 AIM: To investigate whether uncoupling protein 2 (UCP2) affects oleic acid-induced secretion of glucagon-like peptide-1 (GLP-1) in L-cells. Oleic Acid 64-74 uncoupling protein 2 Homo sapiens 28-48 22807616-1 2012 AIM: To investigate whether uncoupling protein 2 (UCP2) affects oleic acid-induced secretion of glucagon-like peptide-1 (GLP-1) in L-cells. Oleic Acid 64-74 uncoupling protein 2 Homo sapiens 50-54 22807616-2 2012 METHODS: mRNA and protein expression of UCP2 were analyzed in human NCI-H716 cells, which serve as a model for enteroendocrine L-cells, by quantitative reverse transcription-polymerase chain reaction and Western blotting before and after treatment with oleic acid. Oleic Acid 253-263 uncoupling protein 2 Homo sapiens 40-44 22807616-11 2012 CONCLUSION: UCP2 affected GLP-1 secretion induced by oleic acid. Oleic Acid 53-63 uncoupling protein 2 Homo sapiens 12-16 22807616-12 2012 UCP2 plays an important role in L-cell secretion that is induced by free fatty acids. Fatty Acids, Nonesterified 68-84 uncoupling protein 2 Homo sapiens 0-4 22257551-0 2012 Uncoupling protein 2 negatively regulates glucose-induced glucagon-like peptide 1 secretion. Glucose 42-49 uncoupling protein 2 Homo sapiens 0-20 22366325-7 2012 In addition, the data suggest that uncoupling protein-2 may be a target for retarding the progressive loss of nigrostriatal dopamine neurons that occurs in Parkinson"s disease and aging. Dopamine 124-132 uncoupling protein 2 Homo sapiens 35-55 22241057-1 2012 Uncoupling proteins 2 and 3 (UCP2 and UCP3) may negatively regulate mitochondrial ATP synthesis and, through this, influence human physical performance. Adenosine Triphosphate 82-85 uncoupling protein 2 Homo sapiens 0-27 22241057-1 2012 Uncoupling proteins 2 and 3 (UCP2 and UCP3) may negatively regulate mitochondrial ATP synthesis and, through this, influence human physical performance. Adenosine Triphosphate 82-85 uncoupling protein 2 Homo sapiens 29-33 22257551-13 2012 Taken together, these results reveal an inhibitory role of UCP2 in glucose-induced GLP1 secretion. Glucose 67-74 uncoupling protein 2 Homo sapiens 59-63 22266335-4 2012 Here we show that hucp2 expression in Drosophila DA neurons under the control of the tyrosine hydroxylase (TH) promoter protects those flies against the mitochondrial toxin rotenone-induced DA neuron death, head dopamine depletion, impaired locomotor activity and energy deficiency. Rotenone 173-181 uncoupling protein 2 Homo sapiens 18-23 22266335-4 2012 Here we show that hucp2 expression in Drosophila DA neurons under the control of the tyrosine hydroxylase (TH) promoter protects those flies against the mitochondrial toxin rotenone-induced DA neuron death, head dopamine depletion, impaired locomotor activity and energy deficiency. Dopamine 212-220 uncoupling protein 2 Homo sapiens 18-23 22266335-5 2012 Under normal conditions, hUCP2 flies maintain an enhanced locomotor activity and have higher steady-state ATP levels suggesting improved energy homeostasis. Adenosine Triphosphate 106-109 uncoupling protein 2 Homo sapiens 25-30 22266335-7 2012 Those results suggest that it is increased mitochondrial function but not mitochondrial biogenesis that appears responsible for higher ATP levels in hUCP2 flies. Adenosine Triphosphate 135-138 uncoupling protein 2 Homo sapiens 149-154 22415082-9 2012 The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. Propofol 69-77 uncoupling protein 2 Homo sapiens 48-52 23210978-9 2012 Furthermore, we previously reported an interesting link between PD and metabolic processes through the protective effects of leptin (hormone produced by adipocytes) acting via UCP2 against MPP+-induced toxicity. mangion-purified polysaccharide (Candida albicans) 189-192 uncoupling protein 2 Homo sapiens 176-180 22070905-10 2012 Malondialdehyde levels were significantly elevated in subjects with DD genotype of UCP-2 I/D (p < 0.05) and CC genotype of manganese superoxide dismutase (p < 0.05) as compared with II or ID and TT or CT genotype, respectively. Malondialdehyde 0-15 uncoupling protein 2 Homo sapiens 83-88 22464786-0 2012 [Establishment of the Chang liver cell line stably overexpressing human UCP2 gene and its effect on mitochondrial membrane potential and reactive oxygen species]. Reactive Oxygen Species 137-160 uncoupling protein 2 Homo sapiens 72-76 22464786-11 2012 Using this cell system, UCP2 was found to play a role in mitochondrial function by regulating MMP and ROS. Reactive Oxygen Species 102-105 uncoupling protein 2 Homo sapiens 24-28 22415082-9 2012 The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. Sevoflurane 83-94 uncoupling protein 2 Homo sapiens 48-52 22526333-8 2012 In parallel, FasL induces upregulation of the uncoupling protein UCP2, the main uncoupling protein in MSCs, which is not abrogated by EGF; however, the production of ROS is followed by a delayed apoptotic cell death despite moderation by UCP2. Reactive Oxygen Species 166-169 uncoupling protein 2 Homo sapiens 65-69 22414059-5 2012 In addition, ROS activate UCP2 via peroxidation of the mitochondrial membrane phospholipids, which results in proton leak leading to reduced ATP synthesis and content in beta-cells - critical parameters in the regulation of glucose-stimulated insulin secretion. Adenosine Triphosphate 141-144 uncoupling protein 2 Homo sapiens 26-30 22414059-5 2012 In addition, ROS activate UCP2 via peroxidation of the mitochondrial membrane phospholipids, which results in proton leak leading to reduced ATP synthesis and content in beta-cells - critical parameters in the regulation of glucose-stimulated insulin secretion. Glucose 224-231 uncoupling protein 2 Homo sapiens 26-30 22414059-5 2012 In addition, ROS activate UCP2 via peroxidation of the mitochondrial membrane phospholipids, which results in proton leak leading to reduced ATP synthesis and content in beta-cells - critical parameters in the regulation of glucose-stimulated insulin secretion. Reactive Oxygen Species 13-16 uncoupling protein 2 Homo sapiens 26-30 22134120-1 2012 Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. Adenosine Triphosphate 148-151 uncoupling protein 2 Homo sapiens 0-20 22110477-5 2012 ROS activate UCP2, which results in proton leak across the mitochondrial inner membrane, and this leads to reduced beta-cell ATP synthesis and content, which is a critical parameter in regulating glucose-stimulated insulin secretion. Reactive Oxygen Species 0-3 uncoupling protein 2 Homo sapiens 13-17 22134120-1 2012 Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. Adenosine Triphosphate 148-151 uncoupling protein 2 Homo sapiens 22-26 22110477-5 2012 ROS activate UCP2, which results in proton leak across the mitochondrial inner membrane, and this leads to reduced beta-cell ATP synthesis and content, which is a critical parameter in regulating glucose-stimulated insulin secretion. Adenosine Triphosphate 125-128 uncoupling protein 2 Homo sapiens 13-17 22134120-1 2012 Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. Adenosine Triphosphate 241-244 uncoupling protein 2 Homo sapiens 0-20 22110477-5 2012 ROS activate UCP2, which results in proton leak across the mitochondrial inner membrane, and this leads to reduced beta-cell ATP synthesis and content, which is a critical parameter in regulating glucose-stimulated insulin secretion. Glucose 196-203 uncoupling protein 2 Homo sapiens 13-17 22134120-1 2012 Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. Adenosine Triphosphate 241-244 uncoupling protein 2 Homo sapiens 22-26 22134120-2 2012 As a consequence of the uncoupling, UCP2 decreases the reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 55-78 uncoupling protein 2 Homo sapiens 36-40 22134120-2 2012 As a consequence of the uncoupling, UCP2 decreases the reactive oxygen species (ROS) formation by mitochondria. Reactive Oxygen Species 80-83 uncoupling protein 2 Homo sapiens 36-40 23029600-5 2012 The mitochondrial uncoupling protein UCP2, even though its uncoupling properties are debated, has been associated with protective functions against ROS toxicity. ros 148-151 uncoupling protein 2 Homo sapiens 37-41 22393835-0 2012 Effect of genetic polymorphism of UCP2-866 G/A on repaglinide response in Chinese patients with type 2 diabetes. repaglinide 50-61 uncoupling protein 2 Homo sapiens 34-38 22393835-1 2012 The aim of the present study was to evaluate the impact of the UCP2-866 G/A polymorphism on the efficacy of repaglinide in treating patients with diabetes mellitus type 2 (T2DM). repaglinide 108-119 uncoupling protein 2 Homo sapiens 63-67 22393835-3 2012 16 patients with GG genotype, 14 with GA genotype and 11 with AA genotype of UCP2-866 G/A underwent an 8-week repaglinide treatment regimen. repaglinide 110-121 uncoupling protein 2 Homo sapiens 77-81 22393835-5 2012 The patient with AA genotype of UCP2-866 G/A had higher levels of fasting plasma glucose (FPG), 30-min and 2-h postload plasma glucose, glycated haemoglobin (HbA1c), and lower concentrations of 30-min and 2-h postload plasma insulin, homeostasis model assessment of beta cell function (HOMA-beta), deltaI30/deltaG30 compared with GG genotype. Glucose 81-88 uncoupling protein 2 Homo sapiens 32-36 22393835-5 2012 The patient with AA genotype of UCP2-866 G/A had higher levels of fasting plasma glucose (FPG), 30-min and 2-h postload plasma glucose, glycated haemoglobin (HbA1c), and lower concentrations of 30-min and 2-h postload plasma insulin, homeostasis model assessment of beta cell function (HOMA-beta), deltaI30/deltaG30 compared with GG genotype. Glucose 127-134 uncoupling protein 2 Homo sapiens 32-36 22393835-6 2012 After repaglinide treatment for 8 consecutive weeks, we found that A allele carriers of UCP2 in the T2DM patients had smaller decrease in FPG (P < 0.05) and HbA1c (P < 0.05), and smaller increase in 30-min postload plasma insulin (P < 0.01) compared with GG genotypes. repaglinide 6-17 uncoupling protein 2 Homo sapiens 88-92 22393835-7 2012 We demonstrated that UCP2-866 G/A polymorphism is associated with the therapeutic efficacy of repaglinide in Chinese T2DM patients. repaglinide 94-105 uncoupling protein 2 Homo sapiens 21-25 22414059-5 2012 In addition, ROS activate UCP2 via peroxidation of the mitochondrial membrane phospholipids, which results in proton leak leading to reduced ATP synthesis and content in beta-cells - critical parameters in the regulation of glucose-stimulated insulin secretion. Phospholipids 78-91 uncoupling protein 2 Homo sapiens 26-30 22292025-0 2012 UCP2 inhibits ROS-mediated apoptosis in A549 under hypoxic conditions. Reactive Oxygen Species 14-17 uncoupling protein 2 Homo sapiens 0-4 22292025-4 2012 Over-expression of UCP2 in A549 cells inhibited reactive oxygen species (ROS) accumulation (P<0.001) and apoptosis (P<0.001) compared to the controls when the cells were exposed to hypoxia. Reactive Oxygen Species 48-71 uncoupling protein 2 Homo sapiens 19-23 22292025-4 2012 Over-expression of UCP2 in A549 cells inhibited reactive oxygen species (ROS) accumulation (P<0.001) and apoptosis (P<0.001) compared to the controls when the cells were exposed to hypoxia. Reactive Oxygen Species 73-76 uncoupling protein 2 Homo sapiens 19-23 22292025-6 2012 Conversely, suppression of UCP2 resulted in the ROS generation (P = 0.006), the induction of apoptosis (P<0.001), and the release of cytochrome C from mitochondria to the cytosolic fraction, thus activating caspase-9. Reactive Oxygen Species 48-51 uncoupling protein 2 Homo sapiens 27-31 22292025-7 2012 These data suggest that over-expression of UCP2 has anti-apoptotic properties by inhibiting ROS-mediated apoptosis in A549 cells under hypoxic conditions. Reactive Oxygen Species 92-95 uncoupling protein 2 Homo sapiens 43-47 21917523-4 2012 Mitochondrial uncoupling protein 2 (UCP2) has been implicated in physiological and pathological processes related to glucose and lipid metabolism, and in this review we discuss the latest data on the relationships between UCP2 and glucose and lipid sensing from the perspective of specific hypothalamic neuronal circuits and peripheral tissue functions. Glucose 117-124 uncoupling protein 2 Homo sapiens 0-34 21917523-4 2012 Mitochondrial uncoupling protein 2 (UCP2) has been implicated in physiological and pathological processes related to glucose and lipid metabolism, and in this review we discuss the latest data on the relationships between UCP2 and glucose and lipid sensing from the perspective of specific hypothalamic neuronal circuits and peripheral tissue functions. Glucose 117-124 uncoupling protein 2 Homo sapiens 36-40 21917523-4 2012 Mitochondrial uncoupling protein 2 (UCP2) has been implicated in physiological and pathological processes related to glucose and lipid metabolism, and in this review we discuss the latest data on the relationships between UCP2 and glucose and lipid sensing from the perspective of specific hypothalamic neuronal circuits and peripheral tissue functions. Glucose 231-238 uncoupling protein 2 Homo sapiens 0-34 21917523-4 2012 Mitochondrial uncoupling protein 2 (UCP2) has been implicated in physiological and pathological processes related to glucose and lipid metabolism, and in this review we discuss the latest data on the relationships between UCP2 and glucose and lipid sensing from the perspective of specific hypothalamic neuronal circuits and peripheral tissue functions. Glucose 231-238 uncoupling protein 2 Homo sapiens 36-40 21917523-4 2012 Mitochondrial uncoupling protein 2 (UCP2) has been implicated in physiological and pathological processes related to glucose and lipid metabolism, and in this review we discuss the latest data on the relationships between UCP2 and glucose and lipid sensing from the perspective of specific hypothalamic neuronal circuits and peripheral tissue functions. Glucose 231-238 uncoupling protein 2 Homo sapiens 222-226 22085932-6 2011 Uncoupling protein 2 (UCP2) plays a regulating role in hPSC energy metabolism by preventing mitochondrial glucose oxidation and facilitating glycolysis via a substrate shunting mechanism. Glucose 106-113 uncoupling protein 2 Homo sapiens 0-20 22085932-6 2011 Uncoupling protein 2 (UCP2) plays a regulating role in hPSC energy metabolism by preventing mitochondrial glucose oxidation and facilitating glycolysis via a substrate shunting mechanism. Glucose 106-113 uncoupling protein 2 Homo sapiens 22-26 22085932-7 2011 With early differentiation, hPSC proliferation slows, energy metabolism decreases, and UCP2 is repressed, resulting in decreased glycolysis and maintained or increased mitochondrial glucose oxidation. Glucose 182-189 uncoupling protein 2 Homo sapiens 87-91 21762777-5 2011 However, UCPs 2 and 3 are generally thought to be activated by ROS or ROS by-products to induce proton leak, thus providing a negative feedback loop for mitochondrial ROS production. Reactive Oxygen Species 63-66 uncoupling protein 2 Homo sapiens 9-21 21917523-0 2012 Mitochondrial uncoupling protein 2 (UCP2) in glucose and lipid metabolism. Glucose 45-52 uncoupling protein 2 Homo sapiens 0-34 21917523-0 2012 Mitochondrial uncoupling protein 2 (UCP2) in glucose and lipid metabolism. Glucose 45-52 uncoupling protein 2 Homo sapiens 36-40 21762777-5 2011 However, UCPs 2 and 3 are generally thought to be activated by ROS or ROS by-products to induce proton leak, thus providing a negative feedback loop for mitochondrial ROS production. Reactive Oxygen Species 70-73 uncoupling protein 2 Homo sapiens 9-21 21762777-5 2011 However, UCPs 2 and 3 are generally thought to be activated by ROS or ROS by-products to induce proton leak, thus providing a negative feedback loop for mitochondrial ROS production. Reactive Oxygen Species 70-73 uncoupling protein 2 Homo sapiens 9-21 21762777-6 2011 In our laboratory, we have not only confirmed that ROS activate UCP2 and UCP3, but also demonstrated that UCP2 and UCP3 are controlled by covalent modification by glutathione. Reactive Oxygen Species 51-54 uncoupling protein 2 Homo sapiens 64-68 21762777-6 2011 In our laboratory, we have not only confirmed that ROS activate UCP2 and UCP3, but also demonstrated that UCP2 and UCP3 are controlled by covalent modification by glutathione. Glutathione 163-174 uncoupling protein 2 Homo sapiens 106-110 21762777-8 2011 Hence, our findings are consistent with the notion that UCPs 2 and 3 are acutely activated by ROS, which then directly modulate the glutathionylation status of the UCP to decrease ROS emission and participate in cell signaling mechanisms. Reactive Oxygen Species 94-97 uncoupling protein 2 Homo sapiens 56-68 21762777-8 2011 Hence, our findings are consistent with the notion that UCPs 2 and 3 are acutely activated by ROS, which then directly modulate the glutathionylation status of the UCP to decrease ROS emission and participate in cell signaling mechanisms. Reactive Oxygen Species 180-183 uncoupling protein 2 Homo sapiens 56-68 21779625-3 2011 Uncoupling protein 2 (UCP2) is expressed in several tissues, and acts in the protection against oxidative stress; in the negative regulation of insulin secretion by beta cells, and in fatty acid metabolism. Fatty Acids 184-194 uncoupling protein 2 Homo sapiens 0-20 21779625-3 2011 Uncoupling protein 2 (UCP2) is expressed in several tissues, and acts in the protection against oxidative stress; in the negative regulation of insulin secretion by beta cells, and in fatty acid metabolism. Fatty Acids 184-194 uncoupling protein 2 Homo sapiens 22-26 21626599-6 2011 These results suggest that capsaicin exerts its lipolytic action by increasing the hydrolysis of triacylglycerol in adipocytes, and that these effects are mediated at least partially by regulation of the expression of multiple genes that are involved in the lipid catabolic pathway, such as HSL and CPT-Ialpha, and those involved in thermogenesis such as UCP2. Capsaicin 27-36 uncoupling protein 2 Homo sapiens 355-359 21321094-0 2011 Uncoupling protein-2 increases nitric oxide production and TNFAIP3 pathway activation in pancreatic islets. Nitric Oxide 31-43 uncoupling protein 2 Homo sapiens 0-20 21539509-4 2011 The existing evidence suggests ghrelin primarily inhibits insulin release from the pancreas and we highlight an important mechanism involving AMPK-UCP2 ATP-stimulated potassium channels and intracellular calcium regulation. Ghrelin 31-38 uncoupling protein 2 Homo sapiens 147-151 21321094-3 2011 Nitric oxide (NO) production was elevated in Ucp2(-/-) islets. Nitric Oxide 0-12 uncoupling protein 2 Homo sapiens 45-49 21374988-0 2010 [Influence of genipin and vitamin E on UCP2 and other correlation factors in non-alcoholic fatty liver disease]. genipin 14-21 uncoupling protein 2 Homo sapiens 39-43 21980456-8 2011 In cultured human umbilical vein endothelial cells (HUVECs), berberine significantly increased UCP2 mRNA and protein expression in an AMPK-dependent manner. Berberine 61-70 uncoupling protein 2 Homo sapiens 95-99 21338923-6 2011 We demonstrate that chromanes also inhibit UCP2 and, in HT-29 human carcinoma cells, cause oxidative stress. chromanes 20-29 uncoupling protein 2 Homo sapiens 43-47 22072942-6 2011 PGC1-alpha is a master regulator of ROS scavenging enzymes including manganese superoxide dismutase 2 and the uncoupling protein 2, both are mitochondrial proteins, and may contribute to neuronal survival. Reactive Oxygen Species 36-39 uncoupling protein 2 Homo sapiens 110-130 21935467-9 2011 Genipin, a plant derived small molecule, suppressed the UCP2 led tumorigenic properties, which were mediated by decreased reactive oxygen species and down-regulation of UCP2. Reactive Oxygen Species 122-145 uncoupling protein 2 Homo sapiens 56-60 21935467-13 2011 In summary, our studies demonstrate that i) the Warburg Effect is mediated by UCP2; ii) UCP2 is over-expressed in breast and many other cancers; iii) UCP2 promotes tumorigenic properties in vitro and in vivo and iv) genipin suppresses the tumor promoting function of UCP2. genipin 216-223 uncoupling protein 2 Homo sapiens 88-92 21935467-13 2011 In summary, our studies demonstrate that i) the Warburg Effect is mediated by UCP2; ii) UCP2 is over-expressed in breast and many other cancers; iii) UCP2 promotes tumorigenic properties in vitro and in vivo and iv) genipin suppresses the tumor promoting function of UCP2. genipin 216-223 uncoupling protein 2 Homo sapiens 88-92 21935467-13 2011 In summary, our studies demonstrate that i) the Warburg Effect is mediated by UCP2; ii) UCP2 is over-expressed in breast and many other cancers; iii) UCP2 promotes tumorigenic properties in vitro and in vivo and iv) genipin suppresses the tumor promoting function of UCP2. genipin 216-223 uncoupling protein 2 Homo sapiens 88-92 21374988-0 2010 [Influence of genipin and vitamin E on UCP2 and other correlation factors in non-alcoholic fatty liver disease]. Vitamin E 26-35 uncoupling protein 2 Homo sapiens 39-43 21035764-0 2010 Glucose stimulation of hypothalamic MCH neurons involves K(ATP) channels, is modulated by UCP2, and regulates peripheral glucose homeostasis. Glucose 0-7 uncoupling protein 2 Homo sapiens 90-94 21035764-5 2010 In the present study, we demonstrate that glucose excitation of MCH-expressing neurons in the lateral hypothalamus is mediated by K(ATP) channels and is negatively regulated by UCP2 (a mitochondrial protein that reduces ATP production), and that glucose sensing by MCH neurons plays an important role in regulating glucose homeostasis. Glucose 42-49 uncoupling protein 2 Homo sapiens 177-181 21047682-6 2010 Alignment of the IML2 sequences revealed that UCP1, UCP2 and UCP3 share a basic amino acid in positions 163, 164 and 167, while only UCP2 and UCP3 contain a second basic residue in positions 168 and 171, respectively. Amino Acids, Basic 74-90 uncoupling protein 2 Homo sapiens 52-56 20967268-0 2010 Genipin-induced inhibition of uncoupling protein-2 sensitizes drug-resistant cancer cells to cytotoxic agents. genipin 0-7 uncoupling protein 2 Homo sapiens 30-50 20976134-7 2010 Moreover, uncoupling protein 2 (UCP2), a potential suppressor of ROS in mitochondria, is important for TPA-induced cell transformation in JB6 cells. Reactive Oxygen Species 65-68 uncoupling protein 2 Homo sapiens 10-30 20976134-7 2010 Moreover, uncoupling protein 2 (UCP2), a potential suppressor of ROS in mitochondria, is important for TPA-induced cell transformation in JB6 cells. Reactive Oxygen Species 65-68 uncoupling protein 2 Homo sapiens 32-36 20976134-7 2010 Moreover, uncoupling protein 2 (UCP2), a potential suppressor of ROS in mitochondria, is important for TPA-induced cell transformation in JB6 cells. Tetradecanoylphorbol Acetate 103-106 uncoupling protein 2 Homo sapiens 10-30 20976134-7 2010 Moreover, uncoupling protein 2 (UCP2), a potential suppressor of ROS in mitochondria, is important for TPA-induced cell transformation in JB6 cells. Tetradecanoylphorbol Acetate 103-106 uncoupling protein 2 Homo sapiens 32-36 20976134-8 2010 UCP2 knock down cells showed enhanced p53 mitochondrial translocation, and were less prone to form colonies in soft agar after TPA treatment. Agar 116-120 uncoupling protein 2 Homo sapiens 0-4 20976134-8 2010 UCP2 knock down cells showed enhanced p53 mitochondrial translocation, and were less prone to form colonies in soft agar after TPA treatment. Tetradecanoylphorbol Acetate 127-130 uncoupling protein 2 Homo sapiens 0-4 20967268-1 2010 Uncoupling protein-2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) production and is employed by drug-resistant cancer cells to mitigate oxidative stress. Reactive Oxygen Species 63-86 uncoupling protein 2 Homo sapiens 0-20 20967268-1 2010 Uncoupling protein-2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) production and is employed by drug-resistant cancer cells to mitigate oxidative stress. Reactive Oxygen Species 63-86 uncoupling protein 2 Homo sapiens 22-26 20967268-1 2010 Uncoupling protein-2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) production and is employed by drug-resistant cancer cells to mitigate oxidative stress. Reactive Oxygen Species 88-91 uncoupling protein 2 Homo sapiens 0-20 20967268-1 2010 Uncoupling protein-2 (UCP2) is known to suppress mitochondrial reactive oxygen species (ROS) production and is employed by drug-resistant cancer cells to mitigate oxidative stress. Reactive Oxygen Species 88-91 uncoupling protein 2 Homo sapiens 22-26 20967268-2 2010 Using the drug-sensitive HL-60 cells and the drug-resistant MX2 subline as model systems, we show that genipin, a UCP2 inhibitor, sensitizes drug-resistant cells to cytotoxic agents. genipin 103-110 uncoupling protein 2 Homo sapiens 114-118 20967268-7 2010 UCP2 accounted for a remarkable 37% of the resting cellular oxygen consumption indicating that the MX2 cells are functionally reliant on this protein. Oxygen 60-66 uncoupling protein 2 Homo sapiens 0-4 20332018-6 2010 UCP2 expression proved to be sensitive to the presence of fatty acids but remains unchanged during the ageing process. Fatty Acids 58-69 uncoupling protein 2 Homo sapiens 0-4 21029311-5 2010 An interesting mitochondrial protein whose main function appears to be the control of ROS is uncoupling protein 2 (UCP2). Reactive Oxygen Species 86-89 uncoupling protein 2 Homo sapiens 93-113 21029311-5 2010 An interesting mitochondrial protein whose main function appears to be the control of ROS is uncoupling protein 2 (UCP2). Reactive Oxygen Species 86-89 uncoupling protein 2 Homo sapiens 115-119 21029311-7 2010 This review will focus on the paradoxical roles of ROS in pancreatic beta-cell function and the regulatory role of UCP2 in ROS signalling and GSIS. Reactive Oxygen Species 123-126 uncoupling protein 2 Homo sapiens 115-119 20595066-7 2010 Additionally, inhibition of uncoupling protein-2 (UCP2) caused cytotoxicity in colon cancer cells via ROS of mitochondrial origin. ros 102-105 uncoupling protein 2 Homo sapiens 28-48 20595066-7 2010 Additionally, inhibition of uncoupling protein-2 (UCP2) caused cytotoxicity in colon cancer cells via ROS of mitochondrial origin. ros 102-105 uncoupling protein 2 Homo sapiens 50-54 20595066-8 2010 In conclusion, we show for the first time that UCP2 knockdown participates in the mechanism of action of cisplatin, thus providing evidence that targeting UCP2 may offer clinical benefit in the treatment of cancer. Cisplatin 105-114 uncoupling protein 2 Homo sapiens 47-51 20595066-8 2010 In conclusion, we show for the first time that UCP2 knockdown participates in the mechanism of action of cisplatin, thus providing evidence that targeting UCP2 may offer clinical benefit in the treatment of cancer. Cisplatin 105-114 uncoupling protein 2 Homo sapiens 155-159 20211596-1 2010 Uncoupling proteins (UCP1, UCP2 and UCP3) are important in regulating cellular fuel metabolism and as attenuators of reactive oxygen species production through strong or mild uncoupling. Reactive Oxygen Species 117-140 uncoupling protein 2 Homo sapiens 27-31 21364658-6 2010 In contrast, poorly differentiated tumor cells, known to be TGFbeta resistant, displayed aberrant UCP2 regulation, and consequently, gene overexpression, which reduced mitochondrial calcium and facilitated the maintenance of mitochondrial membrane potential, thereby significantly decreasing oxidative stress and inhibiting cell death. Calcium 182-189 uncoupling protein 2 Homo sapiens 98-102 20595066-0 2010 Uncoupling protein-2 knockdown mediates the cytotoxic effects of cisplatin. Cisplatin 65-74 uncoupling protein 2 Homo sapiens 0-20 20608355-2 2010 Accumulating evidence indicates that uncoupling protein 2 (UCP2) prevents the apoptosis of multiple types of cells induced by reactive oxygen species (ROS). Reactive Oxygen Species 126-149 uncoupling protein 2 Homo sapiens 37-57 20608355-2 2010 Accumulating evidence indicates that uncoupling protein 2 (UCP2) prevents the apoptosis of multiple types of cells induced by reactive oxygen species (ROS). Reactive Oxygen Species 126-149 uncoupling protein 2 Homo sapiens 59-63 20608355-2 2010 Accumulating evidence indicates that uncoupling protein 2 (UCP2) prevents the apoptosis of multiple types of cells induced by reactive oxygen species (ROS). Reactive Oxygen Species 151-154 uncoupling protein 2 Homo sapiens 37-57 20608355-2 2010 Accumulating evidence indicates that uncoupling protein 2 (UCP2) prevents the apoptosis of multiple types of cells induced by reactive oxygen species (ROS). Reactive Oxygen Species 151-154 uncoupling protein 2 Homo sapiens 59-63 20608355-8 2010 CONCLUSION: UCP2 plays an important protective role against oxidative stress damage to human sperm by diminishing ROS production. Reactive Oxygen Species 114-117 uncoupling protein 2 Homo sapiens 12-16 19846869-0 2009 Mitochondrial uncoupling protein 2 inhibits mast cell activation and reduces histamine content. Histamine 77-86 uncoupling protein 2 Homo sapiens 0-34 19681913-1 2010 BACKGROUND AND OBJECTIVE: Uncoupling protein 2 (UCP2) plays a role in controlling reactive oxygen species (ROS) production by mitochondria. Reactive Oxygen Species 82-105 uncoupling protein 2 Homo sapiens 26-46 19681913-1 2010 BACKGROUND AND OBJECTIVE: Uncoupling protein 2 (UCP2) plays a role in controlling reactive oxygen species (ROS) production by mitochondria. Reactive Oxygen Species 82-105 uncoupling protein 2 Homo sapiens 48-52 19681913-1 2010 BACKGROUND AND OBJECTIVE: Uncoupling protein 2 (UCP2) plays a role in controlling reactive oxygen species (ROS) production by mitochondria. Reactive Oxygen Species 107-110 uncoupling protein 2 Homo sapiens 26-46 19681913-1 2010 BACKGROUND AND OBJECTIVE: Uncoupling protein 2 (UCP2) plays a role in controlling reactive oxygen species (ROS) production by mitochondria. Reactive Oxygen Species 107-110 uncoupling protein 2 Homo sapiens 48-52 19681913-2 2010 As ROS overproduction is related to diabetic retinopathy (DR), UCP2 gene polymorphisms might be involved in the development of this complication. Reactive Oxygen Species 3-6 uncoupling protein 2 Homo sapiens 63-67 20490313-10 2010 On the other hand, our study has shown that UCP2 and UCP4 have important impact on mitochondrial calcium concentration of nerve cells, suggesting that their abnormal expression may involve in the pathogenesis of Alzheimer"s disease. Calcium 97-104 uncoupling protein 2 Homo sapiens 44-48 19763737-0 2010 Mitochondrial uncoupling protein-2 (UCP2) mediates leptin protection against MPP+ toxicity in neuronal cells. mangion-purified polysaccharide (Candida albicans) 77-81 uncoupling protein 2 Homo sapiens 0-34 19763737-0 2010 Mitochondrial uncoupling protein-2 (UCP2) mediates leptin protection against MPP+ toxicity in neuronal cells. mangion-purified polysaccharide (Candida albicans) 77-81 uncoupling protein 2 Homo sapiens 36-40 19763737-7 2010 Stable knockdown of UCP2 expression reduced ATP levels, and abolished leptin protection against MPP+-induced mitochondrial depolarization, ATP deficiency, and cell death, indicating that UCP2 is critical in mediating these neuroprotective effects of leptin against MPP+ toxicity. Adenosine Triphosphate 44-47 uncoupling protein 2 Homo sapiens 20-24 19763737-7 2010 Stable knockdown of UCP2 expression reduced ATP levels, and abolished leptin protection against MPP+-induced mitochondrial depolarization, ATP deficiency, and cell death, indicating that UCP2 is critical in mediating these neuroprotective effects of leptin against MPP+ toxicity. mangion-purified polysaccharide (Candida albicans) 96-100 uncoupling protein 2 Homo sapiens 20-24 19763737-7 2010 Stable knockdown of UCP2 expression reduced ATP levels, and abolished leptin protection against MPP+-induced mitochondrial depolarization, ATP deficiency, and cell death, indicating that UCP2 is critical in mediating these neuroprotective effects of leptin against MPP+ toxicity. mangion-purified polysaccharide (Candida albicans) 96-100 uncoupling protein 2 Homo sapiens 187-191 19763737-7 2010 Stable knockdown of UCP2 expression reduced ATP levels, and abolished leptin protection against MPP+-induced mitochondrial depolarization, ATP deficiency, and cell death, indicating that UCP2 is critical in mediating these neuroprotective effects of leptin against MPP+ toxicity. Adenosine Triphosphate 139-142 uncoupling protein 2 Homo sapiens 20-24 19763737-9 2010 Our findings show that leptin preserves cell survival by maintaining MMP and ATP levels mediated through UCP2 in MPP+-induced toxicity. Adenosine Triphosphate 77-80 uncoupling protein 2 Homo sapiens 105-109 19763737-9 2010 Our findings show that leptin preserves cell survival by maintaining MMP and ATP levels mediated through UCP2 in MPP+-induced toxicity. mangion-purified polysaccharide (Candida albicans) 113-117 uncoupling protein 2 Homo sapiens 105-109 20227410-3 2010 UCP2 controls immune cell activation by modulating MAPK pathways and the production of mitochondrial reactive oxygen species. Reactive Oxygen Species 101-124 uncoupling protein 2 Homo sapiens 0-4 20227410-4 2010 In several models of infection, inflammation and autoimmunity, a regulatory impact of UCP2 was demonstrated by its direct implication in the production of cytokines and nitric oxide and in cell migration. Nitric Oxide 169-181 uncoupling protein 2 Homo sapiens 86-90 20227410-5 2010 In addition, UCP2 is reported as a key protein for oxidation of fatty acids, glutamine and glucose. Fatty Acids 64-75 uncoupling protein 2 Homo sapiens 13-17 20227410-5 2010 In addition, UCP2 is reported as a key protein for oxidation of fatty acids, glutamine and glucose. Glutamine 77-86 uncoupling protein 2 Homo sapiens 13-17 20227410-5 2010 In addition, UCP2 is reported as a key protein for oxidation of fatty acids, glutamine and glucose. Glucose 91-98 uncoupling protein 2 Homo sapiens 13-17 20235330-3 2010 Proton leak stimulated by linoleic acid and inhibited by guanosine diphosphate (GDP) was detected, in a manner that was correlated with protein levels for uncoupling protein 2 (UCP2) in the three fractions. Guanosine Diphosphate 57-78 uncoupling protein 2 Homo sapiens 155-175 20235330-3 2010 Proton leak stimulated by linoleic acid and inhibited by guanosine diphosphate (GDP) was detected, in a manner that was correlated with protein levels for uncoupling protein 2 (UCP2) in the three fractions. Guanosine Diphosphate 57-78 uncoupling protein 2 Homo sapiens 177-181 20235330-3 2010 Proton leak stimulated by linoleic acid and inhibited by guanosine diphosphate (GDP) was detected, in a manner that was correlated with protein levels for uncoupling protein 2 (UCP2) in the three fractions. Guanosine Diphosphate 80-83 uncoupling protein 2 Homo sapiens 155-175 20235330-3 2010 Proton leak stimulated by linoleic acid and inhibited by guanosine diphosphate (GDP) was detected, in a manner that was correlated with protein levels for uncoupling protein 2 (UCP2) in the three fractions. Guanosine Diphosphate 80-83 uncoupling protein 2 Homo sapiens 177-181 21437070-8 2009 Further, expression of mRNA of ACO, medium-chain acyl-CoA dehydrogenase, FAT, and UCP-2 were significantly elevated in serotonin (400 nM)-treated Caco-2 cells compared with cells incubated without serotonin by 28.7%, 30.1%, and 39.2%, respectively. Serotonin 119-128 uncoupling protein 2 Homo sapiens 82-87 20359253-0 2010 Effect of the common -866G/A polymorphism of the uncoupling protein 2 gene on weight loss and body composition under sibutramine therapy in an obese Taiwanese population. sibutramine 117-128 uncoupling protein 2 Homo sapiens 49-69 20359253-3 2010 OBJECTIVE: In this study, our goal was to investigate whether a common SNP, -866G/A (rs659366), in the uncoupling protein 2 (UCP2) gene could influence weight reduction and body composition under sibutramine therapy in an obese Taiwanese population. sibutramine 196-207 uncoupling protein 2 Homo sapiens 103-123 20359253-3 2010 OBJECTIVE: In this study, our goal was to investigate whether a common SNP, -866G/A (rs659366), in the uncoupling protein 2 (UCP2) gene could influence weight reduction and body composition under sibutramine therapy in an obese Taiwanese population. sibutramine 196-207 uncoupling protein 2 Homo sapiens 125-129 20359253-6 2010 RESULTS AND CONCLUSION: By comparing the placebo and sibutramine groups with ANCOVA, our data showed a strong effect of sibutramine on weight loss in the combined UCP2 -866 AA + GA genotype groups (p < 0.001). sibutramine 120-131 uncoupling protein 2 Homo sapiens 163-167 20359253-9 2010 Moreover, a potential gene-gene interaction between UCP2 and GNB3 was identified by multiple linear regression models for the weight loss (p < 0.001) and for the percent fat loss (p = 0.031) in response to sibutramine. sibutramine 209-220 uncoupling protein 2 Homo sapiens 52-56 20359253-10 2010 The results suggest that the UCP2 gene may contribute to weight loss and fat change in response to sibutramine therapy in obese Taiwanese patients. sibutramine 99-110 uncoupling protein 2 Homo sapiens 29-33 20056920-2 2010 UCP2 is thought to protect cardiomyocytes against oxidative stress by dissipating the mitochondrial proton gradient and mitochondrial membrane potential (DeltaPsi(m)), thereby reducing mitochondrial reactive oxygen species generation. Reactive Oxygen Species 199-222 uncoupling protein 2 Homo sapiens 0-4 20056920-3 2010 However, in apparent conflict with its uncoupling role, UCP2 has also been proposed to be essential for mitochondrial Ca(2+) uptake, which could have a protective action by stimulating mitochondrial ATP production. Adenosine Triphosphate 199-202 uncoupling protein 2 Homo sapiens 56-60 20056920-5 2010 METHODS AND RESULTS: Adenoviral-mediated expression of UCP2 caused a mild depression of DeltaPsi(m) and increased the basal rate of oxygen consumption but did not affect total cellular ATP levels. Oxygen 132-138 uncoupling protein 2 Homo sapiens 55-59 20056920-5 2010 METHODS AND RESULTS: Adenoviral-mediated expression of UCP2 caused a mild depression of DeltaPsi(m) and increased the basal rate of oxygen consumption but did not affect total cellular ATP levels. Adenosine Triphosphate 185-188 uncoupling protein 2 Homo sapiens 55-59 20056920-8 2010 Pretreatment with the UCP2-specific inhibitor genipin largely reversed the effects UCP2 expression on mitochondrial Ca(2+) handling, bioenergetics, and oxygen utilization. Oxygen 152-158 uncoupling protein 2 Homo sapiens 22-26 20056920-8 2010 Pretreatment with the UCP2-specific inhibitor genipin largely reversed the effects UCP2 expression on mitochondrial Ca(2+) handling, bioenergetics, and oxygen utilization. Oxygen 152-158 uncoupling protein 2 Homo sapiens 83-87 19889414-2 2010 Therefore, we studied the association of leukocyte telomere length (LTL) with the presence of T2D, as well as the effect on the patients" LTL of plasma oxidative stress and of variation in UCP2, a gene involved in the mitochondrial production of reactive oxygen species. Reactive Oxygen Species 246-269 uncoupling protein 2 Homo sapiens 189-193 19889414-10 2010 The association of the UCP2 functional promoter variant with the LTL implies a link between mitochondrial production of reactive oxygen species and shorter telomere length in T2D. Reactive Oxygen Species 120-143 uncoupling protein 2 Homo sapiens 23-27 21113404-6 2010 The addition of a PPAR-beta agonist (L165,041) for the final 24 hours of 1% treatment resulted in increased levels of UCP-2 mRNA and protein whereas Rosiglitazone induced SIRT1, PGC-1alpha, RXR-alpha, PPAR-alpha, CPT-1b, and UCP-2 mRNA and SIRT1 protein. Rosiglitazone 149-162 uncoupling protein 2 Homo sapiens 225-230 21113404-7 2010 Under hypoxia, Resveratrol induced SIRT1, RXR-alpha, PPAR-alpha mRNA, and PPAR-gamma and UCP-2 protein. Resveratrol 15-26 uncoupling protein 2 Homo sapiens 89-94 19846869-8 2009 Furthermore, Ucp2(-/-) BMMCs also had greater production of both IL-6 and PGD(2) as well as ERK phosphorylation, which is known to regulate PG synthesis. pg 74-76 uncoupling protein 2 Homo sapiens 13-17 19906954-0 2009 Ghrelin promotes and protects nigrostriatal dopamine function via a UCP2-dependent mitochondrial mechanism. Ghrelin 0-7 uncoupling protein 2 Homo sapiens 68-72 19906954-0 2009 Ghrelin promotes and protects nigrostriatal dopamine function via a UCP2-dependent mitochondrial mechanism. Dopamine 44-52 uncoupling protein 2 Homo sapiens 68-72 19906954-7 2009 Ghrelin-induced neuroprotection was dependent on the mitochondrial redox state via uncoupling protein 2 (UCP2)-dependent alterations in mitochondrial respiration, reactive oxygen species production, and biogenesis. Ghrelin 0-7 uncoupling protein 2 Homo sapiens 83-103 19906954-7 2009 Ghrelin-induced neuroprotection was dependent on the mitochondrial redox state via uncoupling protein 2 (UCP2)-dependent alterations in mitochondrial respiration, reactive oxygen species production, and biogenesis. Ghrelin 0-7 uncoupling protein 2 Homo sapiens 105-109 19906954-7 2009 Ghrelin-induced neuroprotection was dependent on the mitochondrial redox state via uncoupling protein 2 (UCP2)-dependent alterations in mitochondrial respiration, reactive oxygen species production, and biogenesis. Reactive Oxygen Species 163-186 uncoupling protein 2 Homo sapiens 83-103 19906954-7 2009 Ghrelin-induced neuroprotection was dependent on the mitochondrial redox state via uncoupling protein 2 (UCP2)-dependent alterations in mitochondrial respiration, reactive oxygen species production, and biogenesis. Reactive Oxygen Species 163-186 uncoupling protein 2 Homo sapiens 105-109 19906954-8 2009 Together, our data reveal that peripheral ghrelin plays an important role in the maintenance and protection of normal nigrostriatal dopamine function by activating UCP2-dependent mitochondrial mechanisms. Ghrelin 42-49 uncoupling protein 2 Homo sapiens 164-168 19846869-2 2009 Uncoupling protein 2 (UCP2) is a mitochondrial protein that inhibits insulin secretion from beta cells, possibly through down-regulation of reactive oxygen species production. Reactive Oxygen Species 140-163 uncoupling protein 2 Homo sapiens 0-20 19846869-2 2009 Uncoupling protein 2 (UCP2) is a mitochondrial protein that inhibits insulin secretion from beta cells, possibly through down-regulation of reactive oxygen species production. Reactive Oxygen Species 140-163 uncoupling protein 2 Homo sapiens 22-26 19846869-6 2009 Ucp2(-/-) BMMCs also had elevated histamine content and histidine decarboxylase expression. Histamine 34-43 uncoupling protein 2 Homo sapiens 0-4 19846869-7 2009 Histamine content was reduced by overexpression of UCP2 or treatment with the mitochondrial-targeted superoxide dismutase-mimetic (TBAP) tetrakis(4-benzoic acid) porphyrin manganese(III). Histamine 0-9 uncoupling protein 2 Homo sapiens 51-55 19602668-4 2009 STC1 induces the expression of mitochondrial UCP2, diminishing mitochondrial membrane potential and superoxide generation; studies in UCP2 null and gp91phox null macrophages suggest that suppression of superoxide by STC1 is UCP2-dependent yet is gp91phox-independent. Superoxides 202-212 uncoupling protein 2 Homo sapiens 45-49 19659999-0 2009 Effects of UCP2 -866 G/A and ADRB3 Trp64Arg on rosiglitazone response in Chinese patients with Type 2 diabetes. Rosiglitazone 47-60 uncoupling protein 2 Homo sapiens 11-15 19950601-4 2009 RESULTS: Both the simple obesity and the normal-weight group had the Ala55Val variants of Ala/Ala, Val/Val and Ala/Val in the UCP2 gene, and the Ala55Val genotype distributions between the two groups was significantly different (chi2=11.97, P< 0.05). Alanine 69-72 uncoupling protein 2 Homo sapiens 126-130 19361273-4 2009 It was found that UCP2 and UCP4 protein levels were upregulated in neo but downregulated in APP and APPsw cells by the superoxide anion. Superoxides 119-135 uncoupling protein 2 Homo sapiens 18-22 19361273-5 2009 Our results show that the superoxide anion can regulate protein levels of UCP2 and UCP4 in SH-SY5Y cells, and the mitochondrial free Ca(2+) shifted their levels, tightly coupled with the protein levels of UCPs. Superoxides 26-42 uncoupling protein 2 Homo sapiens 74-78 19304759-6 2009 The fatty acid pathway, hypothalamic mitochondrial respiration, and uncoupling protein 2 have been outlined as downstream targets of AMPK and mediators of ghrelin"s appetite stimulating effect. Ghrelin 155-162 uncoupling protein 2 Homo sapiens 68-88 19469536-5 2009 PPARalpha, ACO, CPT-1, and UCP-2 gene expressions were increased in vitro by acetate addition to HepG2 cells. Acetates 77-84 uncoupling protein 2 Homo sapiens 27-32 19659999-6 2009 The A allele carriers of UCP2 in the T2DM patients had significantly lower PINS (61.5 +/- 34.3 vs. 41.6 +/- 28.7 mU l(-1), P < 0.01) (37.57, 59.16 vs. 34.82, 49.39) and low-density lipoprotein (LDL)-cholesterol compared with GG genotypes (3.4 +/- 1.1 vs. 2.7 +/- 1.1 mmol l(-1), P < 0.05) (2.64, 3.52 vs. 2.66, 3.15). Cholesterol 202-213 uncoupling protein 2 Homo sapiens 25-29 19659999-1 2009 AIMS: The aim of this study was to explore the impact of UCP2 and ADRB3 genetic polymorphisms on the therapeutic efficacy of rosiglitazone in Chinese Type 2 diabetes (T2DM) patients. Rosiglitazone 125-138 uncoupling protein 2 Homo sapiens 57-61 19514063-0 2009 UCP2, a metabolic sensor coupling glucose oxidation to mitochondrial metabolism? Glucose 34-41 uncoupling protein 2 Homo sapiens 0-4 19659999-7 2009 After rosiglitazone treatment for 12 consecutive weeks, we found that A allele carriers of UCP2 in the T2DM patients had smaller attenuated PINS (-3.82 +/- 13.2 vs.-42.1 +/- 30.7 mU l(-1), P < 0.01) (9.45, 51.31 vs. 0.48, 11.88) and greater attenuated HbA(1c) (-1.85 +/- 1.62 vs.-0.61 +/- 0.80, P < 0.05) (0.14, 1.37 vs. 1.10, 2.38) compared with GG genotypes, and ADRB3 Trp64Arg had greater attenuated serum TG (-3.88 +/- 2.77 vs.-0.24 +/- 1.16 mmol l(-1), P < 0.05) (-0.19, 2.74 vs. 1.19, 1.45) and smaller attenuated LDL-cholesterol (1.08 +/- 1.36 vs.-0.36 +/- 0.99, P < 0.01) (-1.26, 0.78 vs.-1.26, 0.79) as well as reduced enhanced adiponectin (1.57 +/- 1.10 vs. 3.15 +/- 2.12 mmol l(-1), P < 0.05) (1.68, 4.08 vs.-9.18, 11.40) compared with ADRB3 Trp64Trp. Rosiglitazone 6-19 uncoupling protein 2 Homo sapiens 91-95 19659999-7 2009 After rosiglitazone treatment for 12 consecutive weeks, we found that A allele carriers of UCP2 in the T2DM patients had smaller attenuated PINS (-3.82 +/- 13.2 vs.-42.1 +/- 30.7 mU l(-1), P < 0.01) (9.45, 51.31 vs. 0.48, 11.88) and greater attenuated HbA(1c) (-1.85 +/- 1.62 vs.-0.61 +/- 0.80, P < 0.05) (0.14, 1.37 vs. 1.10, 2.38) compared with GG genotypes, and ADRB3 Trp64Arg had greater attenuated serum TG (-3.88 +/- 2.77 vs.-0.24 +/- 1.16 mmol l(-1), P < 0.05) (-0.19, 2.74 vs. 1.19, 1.45) and smaller attenuated LDL-cholesterol (1.08 +/- 1.36 vs.-0.36 +/- 0.99, P < 0.01) (-1.26, 0.78 vs.-1.26, 0.79) as well as reduced enhanced adiponectin (1.57 +/- 1.10 vs. 3.15 +/- 2.12 mmol l(-1), P < 0.05) (1.68, 4.08 vs.-9.18, 11.40) compared with ADRB3 Trp64Trp. Thioguanine 415-417 uncoupling protein 2 Homo sapiens 91-95 19659999-7 2009 After rosiglitazone treatment for 12 consecutive weeks, we found that A allele carriers of UCP2 in the T2DM patients had smaller attenuated PINS (-3.82 +/- 13.2 vs.-42.1 +/- 30.7 mU l(-1), P < 0.01) (9.45, 51.31 vs. 0.48, 11.88) and greater attenuated HbA(1c) (-1.85 +/- 1.62 vs.-0.61 +/- 0.80, P < 0.05) (0.14, 1.37 vs. 1.10, 2.38) compared with GG genotypes, and ADRB3 Trp64Arg had greater attenuated serum TG (-3.88 +/- 2.77 vs.-0.24 +/- 1.16 mmol l(-1), P < 0.05) (-0.19, 2.74 vs. 1.19, 1.45) and smaller attenuated LDL-cholesterol (1.08 +/- 1.36 vs.-0.36 +/- 0.99, P < 0.01) (-1.26, 0.78 vs.-1.26, 0.79) as well as reduced enhanced adiponectin (1.57 +/- 1.10 vs. 3.15 +/- 2.12 mmol l(-1), P < 0.05) (1.68, 4.08 vs.-9.18, 11.40) compared with ADRB3 Trp64Trp. Cholesterol 533-544 uncoupling protein 2 Homo sapiens 91-95 19659999-8 2009 CONCLUSION: UCP2 -866 G/A and ADRB3 Trp64Arg polymorphisms are associated with the therapeutic efficacy of multiple-dose rosiglitazone in Chinese T2DM patients. Rosiglitazone 121-134 uncoupling protein 2 Homo sapiens 12-16 19514063-5 2009 Here, we discuss a potential new function for UCP2, as a carrier involved in the coupling between glucose oxidation and mitochondrial metabolism. Glucose 98-105 uncoupling protein 2 Homo sapiens 46-50 19272350-0 2009 Inhibition of uncoupling protein 2 by genipin reduces insulin-stimulated glucose uptake in 3T3-L1 adipocytes. Glucose 73-80 uncoupling protein 2 Homo sapiens 14-34 19272350-3 2009 In this study, UCP2 was inhibited by genipin in 3T3-L1 adipocytes, which increased mitochondrial membrane potential, intracellular ATP level and production of reactive oxygen species (ROS). Adenosine Triphosphate 131-134 uncoupling protein 2 Homo sapiens 15-19 19272350-3 2009 In this study, UCP2 was inhibited by genipin in 3T3-L1 adipocytes, which increased mitochondrial membrane potential, intracellular ATP level and production of reactive oxygen species (ROS). Reactive Oxygen Species 159-182 uncoupling protein 2 Homo sapiens 15-19 19272350-3 2009 In this study, UCP2 was inhibited by genipin in 3T3-L1 adipocytes, which increased mitochondrial membrane potential, intracellular ATP level and production of reactive oxygen species (ROS). Reactive Oxygen Species 184-187 uncoupling protein 2 Homo sapiens 15-19 19480693-0 2009 Acetoacetate reduces growth and ATP concentration in cancer cell lines which over-express uncoupling protein 2. acetoacetic acid 0-12 uncoupling protein 2 Homo sapiens 90-110 19240738-3 2009 Its physiologic role according to emerging evidences, although still not clear, indicate that distribution of UCP2 may be related to regulation of mitochondria membrane potential (DeltaPsim), production of reactive oxygen species (ROS), preservation of calcium homeostasis, modulation of neuronal activity, and eventually inhibition of cellular damage. Reactive Oxygen Species 206-229 uncoupling protein 2 Homo sapiens 110-114 19240738-3 2009 Its physiologic role according to emerging evidences, although still not clear, indicate that distribution of UCP2 may be related to regulation of mitochondria membrane potential (DeltaPsim), production of reactive oxygen species (ROS), preservation of calcium homeostasis, modulation of neuronal activity, and eventually inhibition of cellular damage. Reactive Oxygen Species 231-234 uncoupling protein 2 Homo sapiens 110-114 19240738-3 2009 Its physiologic role according to emerging evidences, although still not clear, indicate that distribution of UCP2 may be related to regulation of mitochondria membrane potential (DeltaPsim), production of reactive oxygen species (ROS), preservation of calcium homeostasis, modulation of neuronal activity, and eventually inhibition of cellular damage. Calcium 253-260 uncoupling protein 2 Homo sapiens 110-114 19368944-0 2009 Uncoupling protein 2 Ala55Val polymorphism is associated with a higher acute insulin response to glucose. Glucose 97-104 uncoupling protein 2 Homo sapiens 0-20 19368944-2 2009 We hypothesized that 2 UCP2 polymorphisms, a -55C/T (Ala55Val) substitution in exon 4 and an exon 8 insertion, would alter the acute insulin response to glucose (AIRg). Glucose 153-160 uncoupling protein 2 Homo sapiens 23-27 19480693-10 2009 The observed over-expression of UCP2 in cancer lines, but not in controls, provides a plausible molecular mechanism by which acetoacetate spares normal cells but suppresses growth in cancer lines. acetoacetic acid 125-137 uncoupling protein 2 Homo sapiens 32-36 19413946-0 2009 UCP2, not a physiologically relevant uncoupler but a glucose sparing switch impacting ROS production and glucose sensing. Glucose 53-60 uncoupling protein 2 Homo sapiens 0-4 19413946-0 2009 UCP2, not a physiologically relevant uncoupler but a glucose sparing switch impacting ROS production and glucose sensing. ros 86-89 uncoupling protein 2 Homo sapiens 0-4 19413946-0 2009 UCP2, not a physiologically relevant uncoupler but a glucose sparing switch impacting ROS production and glucose sensing. Glucose 105-112 uncoupling protein 2 Homo sapiens 0-4 19413946-2 2009 Accordingly, it was proposed that UCP2 and UCP3 are also uncoupling proteins i.e. protonophores with impact on mitochondrial ROS production and glucose signaling. ros 125-128 uncoupling protein 2 Homo sapiens 34-38 19413946-2 2009 Accordingly, it was proposed that UCP2 and UCP3 are also uncoupling proteins i.e. protonophores with impact on mitochondrial ROS production and glucose signaling. Glucose 144-151 uncoupling protein 2 Homo sapiens 34-38 19073597-0 2009 In vitro evidence suggests that miR-133a-mediated regulation of uncoupling protein 2 (UCP2) is an indispensable step in myogenic differentiation. mir-133a 32-40 uncoupling protein 2 Homo sapiens 64-84 19150400-0 2009 Mitochondrial UCP4 attenuates MPP+ - and dopamine-induced oxidative stress, mitochondrial depolarization, and ATP deficiency in neurons and is interlinked with UCP2 expression. Dopamine 41-49 uncoupling protein 2 Homo sapiens 160-164 19073597-0 2009 In vitro evidence suggests that miR-133a-mediated regulation of uncoupling protein 2 (UCP2) is an indispensable step in myogenic differentiation. mir-133a 32-40 uncoupling protein 2 Homo sapiens 86-90 19202275-0 2009 (-)-Epigallocatechin-3-gallate enhances uncoupling protein 2 gene expression in 3T3-L1 adipocytes. epigallocatechin gallate 0-30 uncoupling protein 2 Homo sapiens 40-60 19137581-4 2009 Uncoupling Protein 2 (UCP2) is an attractive candidate to screen for NTD risk because of its possible role in obesity as well as energy metabolism, type-2 diabetes, and the regulation of reactive oxygen species. Reactive Oxygen Species 187-210 uncoupling protein 2 Homo sapiens 0-20 19137581-4 2009 Uncoupling Protein 2 (UCP2) is an attractive candidate to screen for NTD risk because of its possible role in obesity as well as energy metabolism, type-2 diabetes, and the regulation of reactive oxygen species. Reactive Oxygen Species 187-210 uncoupling protein 2 Homo sapiens 22-26 19202275-1 2009 In this study, we investigated the effects of green tea (-)-epigallocatechin-3-gallate (EGCG) on the mRNA level and promoter activity of uncoupling protein 2 (UCP2), a mitochondrial membrane transporter that regulates energy expenditure and thermogenesis in 3T3-L1 adipocytes. epigallocatechin gallate 56-86 uncoupling protein 2 Homo sapiens 137-157 19202275-1 2009 In this study, we investigated the effects of green tea (-)-epigallocatechin-3-gallate (EGCG) on the mRNA level and promoter activity of uncoupling protein 2 (UCP2), a mitochondrial membrane transporter that regulates energy expenditure and thermogenesis in 3T3-L1 adipocytes. epigallocatechin gallate 56-86 uncoupling protein 2 Homo sapiens 159-163 19202275-1 2009 In this study, we investigated the effects of green tea (-)-epigallocatechin-3-gallate (EGCG) on the mRNA level and promoter activity of uncoupling protein 2 (UCP2), a mitochondrial membrane transporter that regulates energy expenditure and thermogenesis in 3T3-L1 adipocytes. epigallocatechin gallate 88-92 uncoupling protein 2 Homo sapiens 137-157 19202275-1 2009 In this study, we investigated the effects of green tea (-)-epigallocatechin-3-gallate (EGCG) on the mRNA level and promoter activity of uncoupling protein 2 (UCP2), a mitochondrial membrane transporter that regulates energy expenditure and thermogenesis in 3T3-L1 adipocytes. epigallocatechin gallate 88-92 uncoupling protein 2 Homo sapiens 159-163 19202275-2 2009 EGCG up-regulated the UCP2 mRNA level in a dose-dependent manner. epigallocatechin gallate 0-4 uncoupling protein 2 Homo sapiens 22-26 19202275-3 2009 UCP2 promoter activity was significantly stimulated by EGCG treatment, to an extent similar to that seen in mRNA expression. epigallocatechin gallate 55-59 uncoupling protein 2 Homo sapiens 0-4 19202275-4 2009 These results suggest that expression of UCP2 gene is directly regulated by green tea EGCG, which is mediated through the transcriptional activation of its proximal promoter. epigallocatechin gallate 86-90 uncoupling protein 2 Homo sapiens 41-45 19155787-1 2009 OBJECTIVES: The mitochondrial uncoupling proteins UCP2 and UCP3 are implicated in energy metabolism and regulation of reactive oxygen species, which are closely involved in autonomic nervous system function. Reactive Oxygen Species 118-141 uncoupling protein 2 Homo sapiens 50-54 19609759-1 2009 There is an increasing evidence that uncoupling protein-2 (UCP2), a recently identified molecular sensor and suppressor of mitochondrial reactive oxygen species (ROS), plays an important role in -regulating apoptosis in different cell systems. Reactive Oxygen Species 137-160 uncoupling protein 2 Homo sapiens 37-57 19056558-5 2009 Furthermore, rs1042615 was related to diabetes status, glucose, and triglycerides within sex-specific quartiles of dietary fat intake (Q1(Fat)-Q4(Fat)) and BMI (Q1(BMI)-Q4(BMI)). Triglycerides 68-81 uncoupling protein 2 Homo sapiens 161-176 19609759-1 2009 There is an increasing evidence that uncoupling protein-2 (UCP2), a recently identified molecular sensor and suppressor of mitochondrial reactive oxygen species (ROS), plays an important role in -regulating apoptosis in different cell systems. Reactive Oxygen Species 137-160 uncoupling protein 2 Homo sapiens 59-63 19609759-1 2009 There is an increasing evidence that uncoupling protein-2 (UCP2), a recently identified molecular sensor and suppressor of mitochondrial reactive oxygen species (ROS), plays an important role in -regulating apoptosis in different cell systems. Reactive Oxygen Species 162-165 uncoupling protein 2 Homo sapiens 37-57 19609759-1 2009 There is an increasing evidence that uncoupling protein-2 (UCP2), a recently identified molecular sensor and suppressor of mitochondrial reactive oxygen species (ROS), plays an important role in -regulating apoptosis in different cell systems. Reactive Oxygen Species 162-165 uncoupling protein 2 Homo sapiens 59-63 18496642-10 2008 The Ala55Val polymorphism of UCP2 was not associated with incident T2DM in the ARIC cohort. ala55val 4-12 uncoupling protein 2 Homo sapiens 29-33 19910678-1 2009 Uncoupling protein-2 (UCP2) and uncoupling protein-5 (UCP5) are ion carriers located in the inner mitochondrial membrane that mediate a regulated discharge of the proton gradient generated by the respiratory chain and are possibly involved in the protection against free radical production. Free Radicals 266-278 uncoupling protein 2 Homo sapiens 0-20 19910678-1 2009 Uncoupling protein-2 (UCP2) and uncoupling protein-5 (UCP5) are ion carriers located in the inner mitochondrial membrane that mediate a regulated discharge of the proton gradient generated by the respiratory chain and are possibly involved in the protection against free radical production. Free Radicals 266-278 uncoupling protein 2 Homo sapiens 22-26 19287073-6 2008 Reduced mRNA levels of calcineurin A beta (PPP3CB), which regulates inflammatory signalling pathways in immune cells, and of the uncoupling protein 2 (UCP2), which has been suggested to control the production of reactive oxygen species (ROS), were observed in response to 0.25 micromol/l OTA. Reactive Oxygen Species 212-235 uncoupling protein 2 Homo sapiens 129-149 19287073-6 2008 Reduced mRNA levels of calcineurin A beta (PPP3CB), which regulates inflammatory signalling pathways in immune cells, and of the uncoupling protein 2 (UCP2), which has been suggested to control the production of reactive oxygen species (ROS), were observed in response to 0.25 micromol/l OTA. Reactive Oxygen Species 212-235 uncoupling protein 2 Homo sapiens 151-155 19287073-6 2008 Reduced mRNA levels of calcineurin A beta (PPP3CB), which regulates inflammatory signalling pathways in immune cells, and of the uncoupling protein 2 (UCP2), which has been suggested to control the production of reactive oxygen species (ROS), were observed in response to 0.25 micromol/l OTA. Reactive Oxygen Species 237-240 uncoupling protein 2 Homo sapiens 129-149 19287073-6 2008 Reduced mRNA levels of calcineurin A beta (PPP3CB), which regulates inflammatory signalling pathways in immune cells, and of the uncoupling protein 2 (UCP2), which has been suggested to control the production of reactive oxygen species (ROS), were observed in response to 0.25 micromol/l OTA. Reactive Oxygen Species 237-240 uncoupling protein 2 Homo sapiens 151-155 19230380-0 2008 The role of UCP2 and ADP/ATP antiporter in superoxide radical-induced uncoupling in kidney mitochondria. Superoxides 43-61 uncoupling protein 2 Homo sapiens 12-16 18844841-2 2008 Calcitriol also suppresses UCP2 expression via the nVDR and thereby increases energy efficiency. Calcitriol 0-10 uncoupling protein 2 Homo sapiens 27-31 18183618-0 2008 1Alpha,25-dihydroxyvitamin D3 attenuates cyanide-induced neurotoxicity by inhibiting uncoupling protein-2 up-regulation. Calcitriol 0-29 uncoupling protein 2 Homo sapiens 85-105 24149904-10 2008 Key pointsObese individuals possess reduced cardiac autonomic nervous activities, especially sympathetic nervous activity associated with thermogenesis induced by capsaicin.Lower sympathetic nervous activity may associate with -866 G/A variants of UCP2 polymorphism.Capsaicin ingestion, however, may consider as a safe nutrient-aid with no adverse effects of cardiac electrical stability. Capsaicin 266-275 uncoupling protein 2 Homo sapiens 248-252 18795068-2 2008 Uncoupling protein 2 (UcP2) is a mitochondrial protein that can influence the mitochondrial membrane potential and hence the production of reactive oxygen species by mitochondria. Reactive Oxygen Species 139-162 uncoupling protein 2 Homo sapiens 0-20 18795068-2 2008 Uncoupling protein 2 (UcP2) is a mitochondrial protein that can influence the mitochondrial membrane potential and hence the production of reactive oxygen species by mitochondria. Reactive Oxygen Species 139-162 uncoupling protein 2 Homo sapiens 22-26 18703058-4 2008 The inhibition of UCP2 expression by an antisense oligonucleotide enhanced the damaging effects of TNF-alpha. Oligonucleotides 50-65 uncoupling protein 2 Homo sapiens 18-22 18282596-4 2008 However, accumulating data question this mechanism and suggest that UCP2 and UCP3 may play other roles, including carrying free fatty acids from the matrix towards the intermembrane space, or contributing to the mitochondrial Ca(2+) uniport. Fatty Acids, Nonesterified 123-139 uncoupling protein 2 Homo sapiens 68-72 18183618-0 2008 1Alpha,25-dihydroxyvitamin D3 attenuates cyanide-induced neurotoxicity by inhibiting uncoupling protein-2 up-regulation. Cyanides 41-48 uncoupling protein 2 Homo sapiens 85-105 18183618-3 2008 In this cell model, Wy14,643 pretreatment enhanced cyanide-induced cell death, and the increased cell death was linked to up-regulation of uncoupling protein-2 (UCP-2). wy14 20-24 uncoupling protein 2 Homo sapiens 161-166 18324929-0 2008 High circulating levels of RBP4 and mRNA levels of aP2, PGC-1alpha and UCP-2 predict improvement in insulin sensitivity following pioglitazone treatment of drug-naive type 2 diabetic subjects. Pioglitazone 130-142 uncoupling protein 2 Homo sapiens 71-76 18413749-3 2008 We show this critical adaptive response in cancer cells to be linked to uncoupling protein-2 (UCP2), a mitochondrial suppressor of reactive oxygen species (ROS). Reactive Oxygen Species 131-154 uncoupling protein 2 Homo sapiens 72-92 18413749-3 2008 We show this critical adaptive response in cancer cells to be linked to uncoupling protein-2 (UCP2), a mitochondrial suppressor of reactive oxygen species (ROS). Reactive Oxygen Species 131-154 uncoupling protein 2 Homo sapiens 94-98 18413749-3 2008 We show this critical adaptive response in cancer cells to be linked to uncoupling protein-2 (UCP2), a mitochondrial suppressor of reactive oxygen species (ROS). Reactive Oxygen Species 156-159 uncoupling protein 2 Homo sapiens 72-92 18413749-3 2008 We show this critical adaptive response in cancer cells to be linked to uncoupling protein-2 (UCP2), a mitochondrial suppressor of reactive oxygen species (ROS). Reactive Oxygen Species 156-159 uncoupling protein 2 Homo sapiens 94-98 18413749-5 2008 Overexpression of UCP2 in HCT116 human colon cancer cells inhibits ROS accumulation and apoptosis after exposure to chemotherapeutic agents. Reactive Oxygen Species 67-70 uncoupling protein 2 Homo sapiens 18-22 18324929-10 2008 High levels of circulating RBP4 at baseline and adipose tissue expression of aP2, proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1alpha) and uncoupling protein 2 (UCP-2) predicted a good treatment response measured as improvement in insulin-stimulated whole-body glucose uptake after 3 months. Glucose 279-286 uncoupling protein 2 Homo sapiens 179-184 18192542-1 2008 OBJECTIVE: Uncoupling protein 2 (UCP2) is a physiological downregulator of reactive oxygen species generation and plays an antiatherogenic role in the vascular wall. Reactive Oxygen Species 75-98 uncoupling protein 2 Homo sapiens 11-31 18192542-1 2008 OBJECTIVE: Uncoupling protein 2 (UCP2) is a physiological downregulator of reactive oxygen species generation and plays an antiatherogenic role in the vascular wall. Reactive Oxygen Species 75-98 uncoupling protein 2 Homo sapiens 33-37 18082129-0 2008 Uncoupling protein-2 accumulates rapidly in the inner mitochondrial membrane during mitochondrial reactive oxygen stress in macrophages. Oxygen 107-113 uncoupling protein 2 Homo sapiens 0-20 18082129-3 2008 UCP2 functions by an incompletely defined mechanism, to reduce reactive oxygen species production during mitochondrial electron transport. Reactive Oxygen Species 63-86 uncoupling protein 2 Homo sapiens 0-4 18082129-4 2008 We observed that the abundance of UCP2 in macrophages increased rapidly in response to treatments (rotenone, antimycin A and diethyldithiocarbamate) that increased mitochondrial superoxide production, but not in response to superoxide produced outside the mitochondria or in response to H2O2. Rotenone 99-107 uncoupling protein 2 Homo sapiens 34-38 18082129-4 2008 We observed that the abundance of UCP2 in macrophages increased rapidly in response to treatments (rotenone, antimycin A and diethyldithiocarbamate) that increased mitochondrial superoxide production, but not in response to superoxide produced outside the mitochondria or in response to H2O2. Antimycin A 109-120 uncoupling protein 2 Homo sapiens 34-38 18082129-4 2008 We observed that the abundance of UCP2 in macrophages increased rapidly in response to treatments (rotenone, antimycin A and diethyldithiocarbamate) that increased mitochondrial superoxide production, but not in response to superoxide produced outside the mitochondria or in response to H2O2. Ditiocarb 125-147 uncoupling protein 2 Homo sapiens 34-38 18082129-4 2008 We observed that the abundance of UCP2 in macrophages increased rapidly in response to treatments (rotenone, antimycin A and diethyldithiocarbamate) that increased mitochondrial superoxide production, but not in response to superoxide produced outside the mitochondria or in response to H2O2. Superoxides 178-188 uncoupling protein 2 Homo sapiens 34-38 18082129-4 2008 We observed that the abundance of UCP2 in macrophages increased rapidly in response to treatments (rotenone, antimycin A and diethyldithiocarbamate) that increased mitochondrial superoxide production, but not in response to superoxide produced outside the mitochondria or in response to H2O2. Hydrogen Peroxide 287-291 uncoupling protein 2 Homo sapiens 34-38 18082129-7 2008 These findings extend our understanding of the homeostatic function of UCP2 in regulating mitochondrial reactive oxygen production by identifying a feedback loop that senses mitochondrial reactive oxygen production and increases inner mitochondrial membrane UCP2 abundance and activity. reactive oxygen 104-119 uncoupling protein 2 Homo sapiens 71-75 18082129-7 2008 These findings extend our understanding of the homeostatic function of UCP2 in regulating mitochondrial reactive oxygen production by identifying a feedback loop that senses mitochondrial reactive oxygen production and increases inner mitochondrial membrane UCP2 abundance and activity. reactive 104-112 uncoupling protein 2 Homo sapiens 71-75 18082129-7 2008 These findings extend our understanding of the homeostatic function of UCP2 in regulating mitochondrial reactive oxygen production by identifying a feedback loop that senses mitochondrial reactive oxygen production and increases inner mitochondrial membrane UCP2 abundance and activity. Oxygen 113-119 uncoupling protein 2 Homo sapiens 71-75 18082129-8 2008 Reactive oxygen species-induction of UCP2 may facilitate survival of macrophages and retention of function in widely variable tissue environments. Reactive Oxygen Species 0-23 uncoupling protein 2 Homo sapiens 37-41 18068334-0 2008 Taxol-induced mitochondrial stress in melanoma cells is mediated by activation of c-Jun N-terminal kinase (JNK) and p38 pathways via uncoupling protein 2. Paclitaxel 0-5 uncoupling protein 2 Homo sapiens 133-153 18068334-5 2008 Taxol resulted in the activation of apoptosis signal regulated kinase (ASK)1, c-jun NH(2)-terminal kinase (JNK), p38(MAPK) and extracellular-regulated kinase (ERK) together with the downregulation of uncoupling protein 2 (UCP2). Paclitaxel 0-5 uncoupling protein 2 Homo sapiens 200-220 18068334-5 2008 Taxol resulted in the activation of apoptosis signal regulated kinase (ASK)1, c-jun NH(2)-terminal kinase (JNK), p38(MAPK) and extracellular-regulated kinase (ERK) together with the downregulation of uncoupling protein 2 (UCP2). Paclitaxel 0-5 uncoupling protein 2 Homo sapiens 222-226 18068334-8 2008 Pretreatment of melanoma cells with the JNK inhibitor (SP600125) or the p38 inhibitor (SB203580) blocked taxol-induced UCP2 downregulation, ROS generation and apoptosis, whereas the ERK inhibitor (PD98059) had no such effect. pyrazolanthrone 55-63 uncoupling protein 2 Homo sapiens 119-123 18068334-8 2008 Pretreatment of melanoma cells with the JNK inhibitor (SP600125) or the p38 inhibitor (SB203580) blocked taxol-induced UCP2 downregulation, ROS generation and apoptosis, whereas the ERK inhibitor (PD98059) had no such effect. SB 203580 87-95 uncoupling protein 2 Homo sapiens 119-123 18068334-8 2008 Pretreatment of melanoma cells with the JNK inhibitor (SP600125) or the p38 inhibitor (SB203580) blocked taxol-induced UCP2 downregulation, ROS generation and apoptosis, whereas the ERK inhibitor (PD98059) had no such effect. Paclitaxel 105-110 uncoupling protein 2 Homo sapiens 119-123 18068334-9 2008 Our data provide evidence that taxol-induced mitochondrial stress occurs through the activation of both JNK and p38 pathways, and suggest a novel role for UCP2 in the modulation of taxol-induced apoptosis of melanoma cells. Paclitaxel 181-186 uncoupling protein 2 Homo sapiens 155-159 18167556-2 2008 In the present studies we hypothesize an opposing effect of glucose on the regulation of UCP-2 and UCP-3 in pancreatic islets. Glucose 60-67 uncoupling protein 2 Homo sapiens 89-94 18167556-10 2008 UCP-2 protein expression in human islets was increased approximately 2-fold after high glucose exposure, whereas UCP-3 protein expression was decreased by approximately 40% (p<0.05). Glucose 87-94 uncoupling protein 2 Homo sapiens 0-5 18167556-13 2008 Increased expression of UCP-2 and decreased expression of UCP-3 in humans with chronic hyperglycemia may contribute to impaired glucose-stimulated insulin secretion. Glucose 128-135 uncoupling protein 2 Homo sapiens 24-29 18839467-2 2008 The function of uncoupling protein-2 (UCP2) is still under debate, but it has been suggested to play a role in reduction of mitochondrial reactive oxygen species. Reactive Oxygen Species 138-161 uncoupling protein 2 Homo sapiens 16-36 18839467-2 2008 The function of uncoupling protein-2 (UCP2) is still under debate, but it has been suggested to play a role in reduction of mitochondrial reactive oxygen species. Reactive Oxygen Species 138-161 uncoupling protein 2 Homo sapiens 38-42 18839467-3 2008 In the present study, we investigated whether the 45 bp deletion/insertion (del/ ins) polymorphism in the UCP2 gene is associated with elevated homocysteine levels and whether it might be associated with an increased risk of recurrent venous thrombosis (RVT). Homocysteine 144-156 uncoupling protein 2 Homo sapiens 106-110 18839467-4 2008 METHODS: The 45 bp del/ins polymorphism in the UCP2 gene was genotyped by PCR analysis in 161 RVT cases and 386 controls of Caucasian origin in which fasting- and post-load homocysteine levels were previously determined. Homocysteine 173-185 uncoupling protein 2 Homo sapiens 47-51 18839467-5 2008 Statistical analysis was performed to assess whether the UCP2 45 bp del/ins polymorphism was associated with plasma total homocysteine levels and venous thrombosis risk. Homocysteine 122-134 uncoupling protein 2 Homo sapiens 57-61 18839467-6 2008 RESULTS: Post-load homocysteine levels were positively associated with UCP2 45 bp ins/ins genotype (P = 0.02). Homocysteine 19-31 uncoupling protein 2 Homo sapiens 71-75 17555951-2 2008 The mitochondrial uncoupling protein-2 (UCP2) negatively regulates reactive oxygen species generation. Reactive Oxygen Species 67-90 uncoupling protein 2 Homo sapiens 4-38 17855623-0 2008 Uncoupling protein-2 controls proliferation by promoting fatty acid oxidation and limiting glycolysis-derived pyruvate utilization. Fatty Acids 57-67 uncoupling protein 2 Homo sapiens 0-20 17855623-0 2008 Uncoupling protein-2 controls proliferation by promoting fatty acid oxidation and limiting glycolysis-derived pyruvate utilization. Pyruvic Acid 110-118 uncoupling protein 2 Homo sapiens 0-20 17855623-1 2008 Uncoupling protein-2 (UCP2) belongs to the mitochondrial carrier family and has been thought to be involved in suppressing mitochondrial ROS production through uncoupling mitochondrial respiration from ATP synthesis. ros 137-140 uncoupling protein 2 Homo sapiens 0-20 17855623-1 2008 Uncoupling protein-2 (UCP2) belongs to the mitochondrial carrier family and has been thought to be involved in suppressing mitochondrial ROS production through uncoupling mitochondrial respiration from ATP synthesis. ros 137-140 uncoupling protein 2 Homo sapiens 22-26 17855623-1 2008 Uncoupling protein-2 (UCP2) belongs to the mitochondrial carrier family and has been thought to be involved in suppressing mitochondrial ROS production through uncoupling mitochondrial respiration from ATP synthesis. Adenosine Triphosphate 202-205 uncoupling protein 2 Homo sapiens 0-20 17855623-1 2008 Uncoupling protein-2 (UCP2) belongs to the mitochondrial carrier family and has been thought to be involved in suppressing mitochondrial ROS production through uncoupling mitochondrial respiration from ATP synthesis. Adenosine Triphosphate 202-205 uncoupling protein 2 Homo sapiens 22-26 17855623-3 2008 Instead, Ucp2-/- cells display enhanced proliferation associated with a metabolic switch from fatty acid oxidation to glucose metabolism. Fatty Acids 94-104 uncoupling protein 2 Homo sapiens 9-13 17855623-4 2008 This metabolic switch requires the unrestricted availability of glucose, and Ucp2-/- cells more readily activate autophagy than wild-type cells when deprived of glucose. Glucose 161-168 uncoupling protein 2 Homo sapiens 77-81 17855623-5 2008 Altogether, these results suggest that UCP2 promotes mitochondrial fatty acid oxidation while limiting mitochondrial catabolism of pyruvate. Fatty Acids 67-77 uncoupling protein 2 Homo sapiens 39-43 17855623-5 2008 Altogether, these results suggest that UCP2 promotes mitochondrial fatty acid oxidation while limiting mitochondrial catabolism of pyruvate. Pyruvic Acid 131-139 uncoupling protein 2 Homo sapiens 39-43 17855623-6 2008 The persistence of fatty acid catabolism in Ucp2+/+ cells during a proliferative response correlates with reduced cell proliferation and enhances resistance to glucose starvation-induced autophagy. Fatty Acids 19-29 uncoupling protein 2 Homo sapiens 44-48 17855623-6 2008 The persistence of fatty acid catabolism in Ucp2+/+ cells during a proliferative response correlates with reduced cell proliferation and enhances resistance to glucose starvation-induced autophagy. Glucose 160-167 uncoupling protein 2 Homo sapiens 44-48 17555951-2 2008 The mitochondrial uncoupling protein-2 (UCP2) negatively regulates reactive oxygen species generation. Reactive Oxygen Species 67-90 uncoupling protein 2 Homo sapiens 40-44 17718790-0 2007 Association of n-6 long-chain polyunsaturated fatty acids to -866 G/A genotypes of the human uncoupling protein 2 gene in obese children. n-6 long-chain polyunsaturated fatty acids 15-57 uncoupling protein 2 Homo sapiens 93-113 17701054-0 2007 Interaction between the UCP2-866G/A, mtDNA 10398G/A and PGC1alpha p.Thr394Thr and p.Gly482Ser polymorphisms in type 2 diabetes susceptibility in North Indian population. thr394thr 68-77 uncoupling protein 2 Homo sapiens 24-28 17718790-1 2007 AIM: To investigate the association of plasma fatty acids with the -866 G/A polymorphism of uncoupling protein 2 (UCP2) in obese children. Fatty Acids 46-57 uncoupling protein 2 Homo sapiens 92-112 17718790-1 2007 AIM: To investigate the association of plasma fatty acids with the -866 G/A polymorphism of uncoupling protein 2 (UCP2) in obese children. Fatty Acids 46-57 uncoupling protein 2 Homo sapiens 114-118 19758950-4 2007 In early atherosclerotic lesions, UCP2 seems to fulfil an atheroprotective effect by reducing reactive oxygen species (ROS) production and/or by inhibiting monocyte recruitment. Reactive Oxygen Species 94-117 uncoupling protein 2 Homo sapiens 34-38 17502873-1 2007 OBJECTIVE: Human uncoupling proteins 2 and 3 (UCP2 and UCP3) are two mitochondrial proteins that are involved in the control of metabolism of fatty acid and possibly protect against oxidative damage. Fatty Acids 142-152 uncoupling protein 2 Homo sapiens 46-50 17671740-6 2007 Fatty acid oxidation-related genes, LCAD, HADHalpha, UCP2, ACOX, BOX, CYP2E1, and CYP4A11, were all overexpressed, indicating that oxidation was enhanced in NAFLD, whereas the expression of CTP1a and PPARalpha was decreased. Fatty Acids 0-10 uncoupling protein 2 Homo sapiens 53-57 17573087-5 2007 Wy14,643 produced a concentration- and time-dependent up-regulation of UCP-2 that was linked to enhanced cyanide-induced cell death. Cyanides 105-112 uncoupling protein 2 Homo sapiens 71-76 17573087-6 2007 MK886 (PPARalpha antagonist) or PPARalpha knock-down by RNA interference (RNAi) inhibited PPARalpha activity as shown by the peroxisome proliferator response element-luciferase reporter assay, but only partially decreased up-regulation of UCP-2. MK-886 0-5 uncoupling protein 2 Homo sapiens 239-244 17573087-8 2007 Wy14,643 induced a rapid surge of ROS generation and loading cells with glutathione ethyl ester (GSH-EE) or pre-treatment with vitamin E attenuated up-regulation of UCP-2. S-ethyl glutathione 72-95 uncoupling protein 2 Homo sapiens 165-170 17573087-8 2007 Wy14,643 induced a rapid surge of ROS generation and loading cells with glutathione ethyl ester (GSH-EE) or pre-treatment with vitamin E attenuated up-regulation of UCP-2. Glutathione 97-100 uncoupling protein 2 Homo sapiens 165-170 17573087-8 2007 Wy14,643 induced a rapid surge of ROS generation and loading cells with glutathione ethyl ester (GSH-EE) or pre-treatment with vitamin E attenuated up-regulation of UCP-2. Vitamin E 127-136 uncoupling protein 2 Homo sapiens 165-170 17573087-10 2007 Co-treatment with PPARalpha-RNAi and GSH-EE blocked both the up-regulation of UCP-2 by Wy14,643 and the cyanide-induced cell death. Glutathione 37-40 uncoupling protein 2 Homo sapiens 78-83 17573087-11 2007 It was concluded that a PPARalpha-mediated pathway and an oxidative stress pathway independent of PPARalpha mediate the up-regulation of UCP-2 and subsequent increased vulnerability to cyanide-induced cytotoxicity. Cyanides 185-192 uncoupling protein 2 Homo sapiens 137-142 17544366-0 2007 Effects of genetic polymorphisms of UCP2 and UCP3 on very low calorie diet-induced body fat reduction in Korean female subjects. calorie 62-69 uncoupling protein 2 Homo sapiens 36-40 17543901-5 2007 Only the PPARdelta ligand GW501516, but not PPARalpha ligand Wy-14,643 or PPARgamma ligand rosiglitazone, significantly increased PPAR-dependent promoter activity and expression of the PPAR-responsive gene UCP2 ( approximately 5-fold). GW 501516 26-34 uncoupling protein 2 Homo sapiens 206-210 19758950-4 2007 In early atherosclerotic lesions, UCP2 seems to fulfil an atheroprotective effect by reducing reactive oxygen species (ROS) production and/or by inhibiting monocyte recruitment. Reactive Oxygen Species 119-122 uncoupling protein 2 Homo sapiens 34-38 17712111-1 2007 OBJECTIVE: We have previously shown 1alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to inhibit mitochondrial uncoupling protein 2 (UCP2) expression in adipocytes and that in vivo suppression of calcitriol levels with calcium-rich diets increases UCP2 expression. Calcitriol 36-65 uncoupling protein 2 Homo sapiens 97-131 17712111-1 2007 OBJECTIVE: We have previously shown 1alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to inhibit mitochondrial uncoupling protein 2 (UCP2) expression in adipocytes and that in vivo suppression of calcitriol levels with calcium-rich diets increases UCP2 expression. Calcitriol 36-65 uncoupling protein 2 Homo sapiens 133-137 17712111-1 2007 OBJECTIVE: We have previously shown 1alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to inhibit mitochondrial uncoupling protein 2 (UCP2) expression in adipocytes and that in vivo suppression of calcitriol levels with calcium-rich diets increases UCP2 expression. Calcitriol 36-65 uncoupling protein 2 Homo sapiens 248-252 17712111-1 2007 OBJECTIVE: We have previously shown 1alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to inhibit mitochondrial uncoupling protein 2 (UCP2) expression in adipocytes and that in vivo suppression of calcitriol levels with calcium-rich diets increases UCP2 expression. Calcitriol 67-84 uncoupling protein 2 Homo sapiens 97-131 17712111-1 2007 OBJECTIVE: We have previously shown 1alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to inhibit mitochondrial uncoupling protein 2 (UCP2) expression in adipocytes and that in vivo suppression of calcitriol levels with calcium-rich diets increases UCP2 expression. Calcitriol 67-84 uncoupling protein 2 Homo sapiens 133-137 17712111-1 2007 OBJECTIVE: We have previously shown 1alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to inhibit mitochondrial uncoupling protein 2 (UCP2) expression in adipocytes and that in vivo suppression of calcitriol levels with calcium-rich diets increases UCP2 expression. Calcitriol 67-84 uncoupling protein 2 Homo sapiens 248-252 16872578-11 2006 Induction of UCP2 expression by glutamine strengthens the proposal that new UCPs could act to determine the choice of mitochondrial substrate. Glutamine 32-41 uncoupling protein 2 Homo sapiens 13-17 17712111-1 2007 OBJECTIVE: We have previously shown 1alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to inhibit mitochondrial uncoupling protein 2 (UCP2) expression in adipocytes and that in vivo suppression of calcitriol levels with calcium-rich diets increases UCP2 expression. Calcitriol 196-206 uncoupling protein 2 Homo sapiens 133-137 17712111-1 2007 OBJECTIVE: We have previously shown 1alpha,25-dihydroxyvitamin D3 [1alpha,25-(OH)2D3] to inhibit mitochondrial uncoupling protein 2 (UCP2) expression in adipocytes and that in vivo suppression of calcitriol levels with calcium-rich diets increases UCP2 expression. Calcium 219-226 uncoupling protein 2 Homo sapiens 133-137 17712111-2 2007 Because UCP2 plays a significant role in the clearance of reactive oxygen species (ROS), we studied the effect of calcitriol on ROS production and ROS-induced adipocyte proliferation. Reactive Oxygen Species 58-81 uncoupling protein 2 Homo sapiens 8-12 17712111-2 2007 Because UCP2 plays a significant role in the clearance of reactive oxygen species (ROS), we studied the effect of calcitriol on ROS production and ROS-induced adipocyte proliferation. Reactive Oxygen Species 83-86 uncoupling protein 2 Homo sapiens 8-12 17712111-5 2007 These effects were augmented by the addition of mitochondrial uncoupling inhibitor guanosine 5"-diphosphate (GDP; 100 microM) or 1alpha,25-(OH)2D3 (10 nM) and attenuated by UCP2 overexpression, suggesting that inhibition of mitochondrial uncoupling suppresses clearance of ROS and increases adipocyte proliferation. Guanosine Diphosphate 83-107 uncoupling protein 2 Homo sapiens 173-177 17712111-5 2007 These effects were augmented by the addition of mitochondrial uncoupling inhibitor guanosine 5"-diphosphate (GDP; 100 microM) or 1alpha,25-(OH)2D3 (10 nM) and attenuated by UCP2 overexpression, suggesting that inhibition of mitochondrial uncoupling suppresses clearance of ROS and increases adipocyte proliferation. Guanosine Diphosphate 109-112 uncoupling protein 2 Homo sapiens 173-177 17712111-5 2007 These effects were augmented by the addition of mitochondrial uncoupling inhibitor guanosine 5"-diphosphate (GDP; 100 microM) or 1alpha,25-(OH)2D3 (10 nM) and attenuated by UCP2 overexpression, suggesting that inhibition of mitochondrial uncoupling suppresses clearance of ROS and increases adipocyte proliferation. Reactive Oxygen Species 273-276 uncoupling protein 2 Homo sapiens 173-177 17712111-8 2007 DISCUSSION: These data support a role of 1alpha,25-(OH)2D3, UCP2, and [Ca2+]i in the regulation of adipocyte ROS production. Reactive Oxygen Species 109-112 uncoupling protein 2 Homo sapiens 60-64 17463068-2 2007 UCP2 is a member of the mitochondrial proton transport family, which uncouples proton entry in the mitochondrial matrix from ATP synthesis. Adenosine Triphosphate 125-128 uncoupling protein 2 Homo sapiens 0-4 17242157-4 2007 It is shown that despite apparently different physiological functions, hUCP2 exhibited its protonophoric function similar to hUCP1--exclusively in the presence of long-chain fatty acids (FA). long-chain fatty acids 163-185 uncoupling protein 2 Homo sapiens 71-76 17985659-10 2007 In conclusion, a single nucleotide polymorphism in the promoter region of the UCP2 gene has a significant association with HDL cholesterol level in Iranian nonobese nondiabetic subjects. Cholesterol 127-138 uncoupling protein 2 Homo sapiens 78-82 17916951-0 2007 Increased plasma manganese, partially reduced ascorbate, 1 and absence of mitochondrial oxidative stress in type 2 diabetes mellitus: implications for the superoxide uncoupling protein 2 (UCP-2) pathway. Superoxides 155-165 uncoupling protein 2 Homo sapiens 166-186 17916951-0 2007 Increased plasma manganese, partially reduced ascorbate, 1 and absence of mitochondrial oxidative stress in type 2 diabetes mellitus: implications for the superoxide uncoupling protein 2 (UCP-2) pathway. Superoxides 155-165 uncoupling protein 2 Homo sapiens 188-193 17916951-2 2007 Manganese (Mn), the key component of the Mitochondrial antioxidant (MnSOD), plays a key role in the superoxide uncoupling protein 2 (UCP-2) pathway in inhibiting of glucose-stimulated insulin secretion (GSIS). Manganese 0-9 uncoupling protein 2 Homo sapiens 111-131 17916951-2 2007 Manganese (Mn), the key component of the Mitochondrial antioxidant (MnSOD), plays a key role in the superoxide uncoupling protein 2 (UCP-2) pathway in inhibiting of glucose-stimulated insulin secretion (GSIS). Manganese 0-9 uncoupling protein 2 Homo sapiens 133-138 17916951-2 2007 Manganese (Mn), the key component of the Mitochondrial antioxidant (MnSOD), plays a key role in the superoxide uncoupling protein 2 (UCP-2) pathway in inhibiting of glucose-stimulated insulin secretion (GSIS). Superoxides 100-110 uncoupling protein 2 Homo sapiens 111-131 17916951-2 2007 Manganese (Mn), the key component of the Mitochondrial antioxidant (MnSOD), plays a key role in the superoxide uncoupling protein 2 (UCP-2) pathway in inhibiting of glucose-stimulated insulin secretion (GSIS). Superoxides 100-110 uncoupling protein 2 Homo sapiens 133-138 17916951-2 2007 Manganese (Mn), the key component of the Mitochondrial antioxidant (MnSOD), plays a key role in the superoxide uncoupling protein 2 (UCP-2) pathway in inhibiting of glucose-stimulated insulin secretion (GSIS). Glucose 165-172 uncoupling protein 2 Homo sapiens 111-131 17916951-2 2007 Manganese (Mn), the key component of the Mitochondrial antioxidant (MnSOD), plays a key role in the superoxide uncoupling protein 2 (UCP-2) pathway in inhibiting of glucose-stimulated insulin secretion (GSIS). Glucose 165-172 uncoupling protein 2 Homo sapiens 133-138 17189204-4 2007 Fasting increased DII activity and local thyroid hormone production in the arcuate nucleus in parallel with increased GDP-regulated UCP2-dependent mitochondrial uncoupling. Guanosine Diphosphate 118-121 uncoupling protein 2 Homo sapiens 132-136 16982633-3 2007 Uncoupling protein 2 (UCP2) uncouples respiration from ATP synthesis, thus regulating energy expenditure and fat oxidation. Adenosine Triphosphate 55-58 uncoupling protein 2 Homo sapiens 0-20 16982633-3 2007 Uncoupling protein 2 (UCP2) uncouples respiration from ATP synthesis, thus regulating energy expenditure and fat oxidation. Adenosine Triphosphate 55-58 uncoupling protein 2 Homo sapiens 22-26 16982633-10 2007 CONCLUSIONS: PD patients, but not HD patients, with the UCP2 del/del genotype showed a significant increase in total and truncal fat mass during the first year of dialysis therapy, suggesting a possible role for UCP2 in dissipating the excess energy of a high-glucose environment. Glucose 260-267 uncoupling protein 2 Homo sapiens 56-60 16968550-5 2006 There is strong evidence that both UCP2 and UCP3 protect against mitochondrial oxidative damage by reducing the production of reactive oxygen species. Reactive Oxygen Species 126-149 uncoupling protein 2 Homo sapiens 35-39 16968550-6 2006 The evidence that UCP2 protein is a negative regulator of insulin secretion by pancreatic beta-cells is also strong: increased UCP2 decreases glucose stimulated insulin secretion ultimately leading to beta-cell dysfunction. Glucose 142-149 uncoupling protein 2 Homo sapiens 18-22 16968550-6 2006 The evidence that UCP2 protein is a negative regulator of insulin secretion by pancreatic beta-cells is also strong: increased UCP2 decreases glucose stimulated insulin secretion ultimately leading to beta-cell dysfunction. Glucose 142-149 uncoupling protein 2 Homo sapiens 127-131 17514359-0 2007 Glutamine stimulates translation of uncoupling protein 2mRNA. Glutamine 0-9 uncoupling protein 2 Homo sapiens 36-56 17514359-2 2007 Using cell lines representing natural sites of UCP2 expression (macrophages, colonocytes, pancreatic beta cells), we show that UCP2 expression is stimulated by glutamine at physiological concentrations. Glutamine 160-169 uncoupling protein 2 Homo sapiens 47-51 17514359-2 2007 Using cell lines representing natural sites of UCP2 expression (macrophages, colonocytes, pancreatic beta cells), we show that UCP2 expression is stimulated by glutamine at physiological concentrations. Glutamine 160-169 uncoupling protein 2 Homo sapiens 127-131 17514359-5 2007 Cloning of the 5" UTR of the UCP2 mRNA in front of a GFP cDNA resulted in a reporter gene with which GFP expression could be induced by glutamine. Glutamine 136-145 uncoupling protein 2 Homo sapiens 29-33 17514359-6 2007 An effect of glutamine on translation of a given mRNA has not been identified before, and this is the first evidence for a link between UCP2 and glutamine, an amino acid oxidized by immune cells or intestinal epithelium and playing a role in the control of insulin secretion. Glutamine 145-154 uncoupling protein 2 Homo sapiens 136-140 17666355-3 2007 RESULTS: Women with low UCP2 level had higher ROS level, suggesting an inverse relationship between them (r=-0.578, P<0.01), and their oocyte development was impaired. Reactive Oxygen Species 46-49 uncoupling protein 2 Homo sapiens 24-28 17666355-5 2007 CONCLUSION: The granular cells increase UCP2 expression to suppress the elevation of intracellular ROS level through a feedback mechanism and therefore protect the oocytes against oxidative stress. Reactive Oxygen Species 99-102 uncoupling protein 2 Homo sapiens 40-44 17512314-3 2007 Among the 10 single nucleotide polymorphisms (SNPs) in the UCP2 and UCP3 genes, 2 SNPs in UCP2, -866G>A and +4787C>T (A55V) that were tightly linked (r(2) = 0.97), were significantly associated with decreased HDL cholesterol levels after Bonferroni correction (P = .003 in the recessive model). Cholesterol 219-230 uncoupling protein 2 Homo sapiens 90-94 17240372-3 2007 Heat production by UCP1 in brown adipocytes is generally a long and adaptive phenomenon, whereas control of mitochondrial ROS by UCP2 needs more subtle regulation. ros 122-125 uncoupling protein 2 Homo sapiens 129-133 16782780-0 2006 PPARalpha-mediated upregulation of uncoupling protein-2 switches cyanide-induced apoptosis to necrosis in primary cortical cells. Cyanides 65-72 uncoupling protein 2 Homo sapiens 35-55 16782780-2 2006 Based on the report that Wy14,643, a PPARalpha agonist, can upregulate uncoupling protein-2 (UCP-2), this study was conducted in primary cortical cells to determine if PPARalpha activation enhances cyanide-induced neurotoxicity through changes in the level of UCP-2. wy14 25-29 uncoupling protein 2 Homo sapiens 71-91 16782780-2 2006 Based on the report that Wy14,643, a PPARalpha agonist, can upregulate uncoupling protein-2 (UCP-2), this study was conducted in primary cortical cells to determine if PPARalpha activation enhances cyanide-induced neurotoxicity through changes in the level of UCP-2. wy14 25-29 uncoupling protein 2 Homo sapiens 93-98 16782780-5 2006 The effect of UCP-2 upregulation on the cytotoxic response to cyanide was quantitated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (apoptosis) and propidium iodide staining (necrosis). Cyanides 62-69 uncoupling protein 2 Homo sapiens 14-19 16782780-5 2006 The effect of UCP-2 upregulation on the cytotoxic response to cyanide was quantitated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (apoptosis) and propidium iodide staining (necrosis). deoxyuridine triphosphate 136-140 uncoupling protein 2 Homo sapiens 14-19 16782780-5 2006 The effect of UCP-2 upregulation on the cytotoxic response to cyanide was quantitated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (apoptosis) and propidium iodide staining (necrosis). Propidium 175-191 uncoupling protein 2 Homo sapiens 14-19 16782780-8 2006 Knock down of UCP-2 expression by RNA interference blocked the Wy14,643-mediated enhancement of cyanide-induced mitochondrial dysfunction and the switch of the cell death mode, thus confirming that the response was mediated by upregulation of UCP-2. Cyanides 96-103 uncoupling protein 2 Homo sapiens 14-19 16782780-8 2006 Knock down of UCP-2 expression by RNA interference blocked the Wy14,643-mediated enhancement of cyanide-induced mitochondrial dysfunction and the switch of the cell death mode, thus confirming that the response was mediated by upregulation of UCP-2. Cyanides 96-103 uncoupling protein 2 Homo sapiens 243-248 16782780-9 2006 This study shows that PPARalpha activation can upregulate UCP-2 expression, which in turn enhances cyanide-induced necrotic cell death through an increase of mitochondrial dysfunction. Cyanides 99-106 uncoupling protein 2 Homo sapiens 58-63 16806053-4 2006 Here we examine critically the evidence that UCP1, UCP2 and UCP3 are stimulated by ROS (superoxide) or ROS products (4-hydroxy-2-nonenal), and that the UCPs actually diminish oxidative damage. Reactive Oxygen Species 83-86 uncoupling protein 2 Homo sapiens 51-55 16806053-4 2006 Here we examine critically the evidence that UCP1, UCP2 and UCP3 are stimulated by ROS (superoxide) or ROS products (4-hydroxy-2-nonenal), and that the UCPs actually diminish oxidative damage. Superoxides 88-98 uncoupling protein 2 Homo sapiens 51-55 16806053-4 2006 Here we examine critically the evidence that UCP1, UCP2 and UCP3 are stimulated by ROS (superoxide) or ROS products (4-hydroxy-2-nonenal), and that the UCPs actually diminish oxidative damage. Reactive Oxygen Species 103-106 uncoupling protein 2 Homo sapiens 51-55 16806053-4 2006 Here we examine critically the evidence that UCP1, UCP2 and UCP3 are stimulated by ROS (superoxide) or ROS products (4-hydroxy-2-nonenal), and that the UCPs actually diminish oxidative damage. 4-hydroxy-2-nonenal 117-136 uncoupling protein 2 Homo sapiens 51-55 16487034-3 2006 Unlike UCP1, UCP2 does not seem to be important to thermogenesis or weight control, but appears to have an important role in the regulation of production of reactive oxygen species, inhibition of inflammation, and inhibition of cell death. Reactive Oxygen Species 157-180 uncoupling protein 2 Homo sapiens 13-17 16282353-8 2006 These transcriptional responses are antagonized by orphan nuclear receptor short heterodimer partner overexpression, which might explain its positive effects on glucose-stimulated insulin secretion in beta-cells overexpressing UCP2. Glucose 161-168 uncoupling protein 2 Homo sapiens 227-231 15970480-5 2005 UCP2 is a recently identified fatty acid-responsive mitochondrial inner membrane carrier protein showing wide tissue distribution with a substantially increased presence in fatty liver. Fatty Acids 30-40 uncoupling protein 2 Homo sapiens 0-4 16021520-1 2005 Uncoupling protein 2 (UCP2) is a member of the mitochondrial proton transport family that uncouples proton entry to the mitochondria from ATP synthesis. Adenosine Triphosphate 138-141 uncoupling protein 2 Homo sapiens 0-20 16021520-1 2005 Uncoupling protein 2 (UCP2) is a member of the mitochondrial proton transport family that uncouples proton entry to the mitochondria from ATP synthesis. Adenosine Triphosphate 138-141 uncoupling protein 2 Homo sapiens 22-26 16437349-2 2006 Many anticancer drugs exert their therapeutic action by generating reactive oxygen radicals, which might be countered by the cancer cell through induction of uncoupling protein 2 (UCP-2). reactive oxygen radicals 67-91 uncoupling protein 2 Homo sapiens 158-178 16437349-2 2006 Many anticancer drugs exert their therapeutic action by generating reactive oxygen radicals, which might be countered by the cancer cell through induction of uncoupling protein 2 (UCP-2). reactive oxygen radicals 67-91 uncoupling protein 2 Homo sapiens 180-185 16437349-13 2006 UCP-2as has a 22-nucleotide insertion from the 3" end of intron 3 that introduces an early stop codon in exon 4, which theoretically can produce a protein 79 amino acids long. 22-nucleotide 14-27 uncoupling protein 2 Homo sapiens 0-5 16580138-8 2006 We also found that exercise significantly increased the expression of the mitochondrial uncoupling protein 2, an energy-balancing factor concerned with ATP production and free radical management. Adenosine Triphosphate 152-155 uncoupling protein 2 Homo sapiens 74-108 16103002-6 2005 A partial but immediate decline of mitochondrial ROS production may be triggered by activation of mitochondrial uncoupling, specifically by activation of recruited or constitutively present uncoupling proteins such as UCP2, which may counterbalance the mild oxidative stress. Reactive Oxygen Species 49-52 uncoupling protein 2 Homo sapiens 218-222 15886331-4 2005 The paralogues UCP2 and UCP3 are probably not thermogenic proteins but convey mild uncoupling, which may serve to reduce the rate of mitochondrial reactive oxygen species production. Reactive Oxygen Species 147-170 uncoupling protein 2 Homo sapiens 15-19 15998440-3 2005 METHODS: We investigated the effect of up-regulation of UCP2 in a hepatoblastoma cell line exposed to menadione or hypoxia/re-oxygenation. Vitamin K 3 102-111 uncoupling protein 2 Homo sapiens 56-60 15998440-4 2005 RESULTS: Lipid and protein oxidation was increased in HepG2 cells exposed to ROS but this increase was significantly lower in cells over-expressing UCP2 under identical conditions. Reactive Oxygen Species 77-80 uncoupling protein 2 Homo sapiens 148-152 16115020-7 2005 UCP2 message has been shown to be up-regulated in the CNS by stress signals such as kainate administration or ischemia, and overexpression of UCP2 has been reported to be neuroprotective against oxidative stress in vivo and in vitro, although the exact mechanism has not been fully established. Kainic Acid 84-91 uncoupling protein 2 Homo sapiens 0-4 15937145-0 2005 Up-regulation of uncoupling protein 2 by cyanide is linked with cytotoxicity in mesencephalic cells. Cyanides 41-48 uncoupling protein 2 Homo sapiens 17-37 15937145-1 2005 Uncoupling protein 2 (UCP-2) regulates mitochondrial function by increasing proton leak across the inner membrane to dissociate respiration from ATP synthesis and reduce reactive oxygen species generation. Adenosine Triphosphate 145-148 uncoupling protein 2 Homo sapiens 0-20 15937145-1 2005 Uncoupling protein 2 (UCP-2) regulates mitochondrial function by increasing proton leak across the inner membrane to dissociate respiration from ATP synthesis and reduce reactive oxygen species generation. Adenosine Triphosphate 145-148 uncoupling protein 2 Homo sapiens 22-27 15937145-1 2005 Uncoupling protein 2 (UCP-2) regulates mitochondrial function by increasing proton leak across the inner membrane to dissociate respiration from ATP synthesis and reduce reactive oxygen species generation. Reactive Oxygen Species 170-193 uncoupling protein 2 Homo sapiens 0-20 15937145-1 2005 Uncoupling protein 2 (UCP-2) regulates mitochondrial function by increasing proton leak across the inner membrane to dissociate respiration from ATP synthesis and reduce reactive oxygen species generation. Reactive Oxygen Species 170-193 uncoupling protein 2 Homo sapiens 22-27 15937145-3 2005 In the current study, we show UCP-2-mediated reduction in mitochondrial function contributes to the mitochondrial dysfunction and the necrotic death of primary cultured mesencephalic cells (MCs) after exposure to cyanide, a complex IV inhibitor. Cyanides 213-220 uncoupling protein 2 Homo sapiens 30-35 15937145-5 2005 Treatment with cyanide for 6 h or longer upregulated UCP-2 expression. Cyanides 15-22 uncoupling protein 2 Homo sapiens 53-58 15937145-7 2005 Knockdown with RNAi or transfection with a UCP-2 dominant-negative interfering mutant reduced the cyanide-induced mitochondrial dysfunction and cell death, showing that constitutive expression of UCP-2 plays a role in the response to cyanide. Cyanides 98-105 uncoupling protein 2 Homo sapiens 43-48 15937145-7 2005 Knockdown with RNAi or transfection with a UCP-2 dominant-negative interfering mutant reduced the cyanide-induced mitochondrial dysfunction and cell death, showing that constitutive expression of UCP-2 plays a role in the response to cyanide. Cyanides 98-105 uncoupling protein 2 Homo sapiens 196-201 15937145-7 2005 Knockdown with RNAi or transfection with a UCP-2 dominant-negative interfering mutant reduced the cyanide-induced mitochondrial dysfunction and cell death, showing that constitutive expression of UCP-2 plays a role in the response to cyanide. Cyanides 234-241 uncoupling protein 2 Homo sapiens 43-48 15937145-7 2005 Knockdown with RNAi or transfection with a UCP-2 dominant-negative interfering mutant reduced the cyanide-induced mitochondrial dysfunction and cell death, showing that constitutive expression of UCP-2 plays a role in the response to cyanide. Cyanides 234-241 uncoupling protein 2 Homo sapiens 196-201 15937145-8 2005 Overexpression of UCP-2 by transfection with human full-length cDNA potentiated the cyanide toxicity. Cyanides 84-91 uncoupling protein 2 Homo sapiens 18-23 15937145-9 2005 These findings indicate that UCP-2 can serve as a regulator of mitochondria-mediated necrotic cell death, in which enhanced expression can increase the vulnerability of primary MCs to injury due to complex IV-mediated inhibition by cyanide. mcs 177-180 uncoupling protein 2 Homo sapiens 29-34 15937145-9 2005 These findings indicate that UCP-2 can serve as a regulator of mitochondria-mediated necrotic cell death, in which enhanced expression can increase the vulnerability of primary MCs to injury due to complex IV-mediated inhibition by cyanide. Cyanides 232-239 uncoupling protein 2 Homo sapiens 29-34 16005426-1 2005 The mitochondrial uncoupling proteins UCP2 and UCP3 may be important in attenuating mitochondrial production of reactive oxygen species, in insulin signalling (UCP2), and perhaps in thermogenesis and other processes. Reactive Oxygen Species 112-135 uncoupling protein 2 Homo sapiens 38-42 15927767-9 2005 There is now increasing evidence that the physiological role of the mammalian UCP3 and UCP2 is rather related to lipid oxidation and/or prevention of reactive oxygen species accumulation than to heat production by uncoupling. Reactive Oxygen Species 150-173 uncoupling protein 2 Homo sapiens 87-91 15800031-0 2005 Enhancement of cyanide-induced mitochondrial dysfunction and cortical cell necrosis by uncoupling protein-2. Cyanides 15-22 uncoupling protein 2 Homo sapiens 87-107 15800031-10 2005 Additionally, treatment of UCP-2+ cells with cyclosporin A blocked necrosis, indicating the involvement of mitochondrial permeability pore transition in the necrotic death. Cyclosporine 45-58 uncoupling protein 2 Homo sapiens 27-32 15800031-12 2005 It is concluded that UCP-2 levels influence cellular responses to cyanide-induced mitochondrial dysfunction. Cyanides 66-73 uncoupling protein 2 Homo sapiens 21-26 15654602-9 2005 In particular, UCP-2 expression is increased (probably due to a condition of fuel overload), which leads to lower ATP, decreased ATP/ADP ratio, with consequent reduction of insulin release. Adenosine Triphosphate 114-117 uncoupling protein 2 Homo sapiens 15-20 15905464-2 2005 Uncoupling protein 2 (UCP2) is an important regulator of intracellular reactive oxygen species (ROS) production. Reactive Oxygen Species 71-94 uncoupling protein 2 Homo sapiens 0-20 15905464-2 2005 Uncoupling protein 2 (UCP2) is an important regulator of intracellular reactive oxygen species (ROS) production. Reactive Oxygen Species 71-94 uncoupling protein 2 Homo sapiens 22-26 15905464-2 2005 Uncoupling protein 2 (UCP2) is an important regulator of intracellular reactive oxygen species (ROS) production. Reactive Oxygen Species 96-99 uncoupling protein 2 Homo sapiens 0-20 15905464-2 2005 Uncoupling protein 2 (UCP2) is an important regulator of intracellular reactive oxygen species (ROS) production. Reactive Oxygen Species 96-99 uncoupling protein 2 Homo sapiens 22-26 15905464-5 2005 Moreover, UCP2 inhibited the increase in ROS production and NF-kappaB activation, and apoptosis of HAECs induced by lysophophatidylcholine (LPC) and linoleic acid. Reactive Oxygen Species 41-44 uncoupling protein 2 Homo sapiens 10-14 15905464-5 2005 Moreover, UCP2 inhibited the increase in ROS production and NF-kappaB activation, and apoptosis of HAECs induced by lysophophatidylcholine (LPC) and linoleic acid. lysophophatidylcholine 116-138 uncoupling protein 2 Homo sapiens 10-14 15905464-5 2005 Moreover, UCP2 inhibited the increase in ROS production and NF-kappaB activation, and apoptosis of HAECs induced by lysophophatidylcholine (LPC) and linoleic acid. lpc 140-143 uncoupling protein 2 Homo sapiens 10-14 15905464-5 2005 Moreover, UCP2 inhibited the increase in ROS production and NF-kappaB activation, and apoptosis of HAECs induced by lysophophatidylcholine (LPC) and linoleic acid. Linoleic Acid 149-162 uncoupling protein 2 Homo sapiens 10-14 15905464-9 2005 The data collectively suggest that UCP2, functions as a physiologic regulator of ROS generation in endothelial cells. Reactive Oxygen Species 81-84 uncoupling protein 2 Homo sapiens 35-39 15827742-2 2005 Uncoupling protein 2 (UCP-2) is an important regulator of intracellular reactive oxygen species (ROS) production. Reactive Oxygen Species 72-95 uncoupling protein 2 Homo sapiens 0-20 15827742-2 2005 Uncoupling protein 2 (UCP-2) is an important regulator of intracellular reactive oxygen species (ROS) production. Reactive Oxygen Species 72-95 uncoupling protein 2 Homo sapiens 22-27 15827742-2 2005 Uncoupling protein 2 (UCP-2) is an important regulator of intracellular reactive oxygen species (ROS) production. Reactive Oxygen Species 97-100 uncoupling protein 2 Homo sapiens 0-20 15827742-2 2005 Uncoupling protein 2 (UCP-2) is an important regulator of intracellular reactive oxygen species (ROS) production. Reactive Oxygen Species 97-100 uncoupling protein 2 Homo sapiens 22-27 15827742-3 2005 We hypothesised that UCP-2 functions as an inhibitor of the atherosclerotic process in VSMCs. vsmcs 87-92 uncoupling protein 2 Homo sapiens 21-26 15604415-2 2005 Uncoupling protein 2 (UCP2) reduces mitochondrial ROS generation and protects against the disease in animal models. Reactive Oxygen Species 50-53 uncoupling protein 2 Homo sapiens 0-20 15604415-2 2005 Uncoupling protein 2 (UCP2) reduces mitochondrial ROS generation and protects against the disease in animal models. Reactive Oxygen Species 50-53 uncoupling protein 2 Homo sapiens 22-26 15827742-10 2005 CONCLUSIONS/INTERPRETATION: The present study demonstrates that UCP-2 can modify atherosclerotic processes in HVSMCs in response to high glucose and Ang II. Glucose 137-144 uncoupling protein 2 Homo sapiens 64-69 15654602-9 2005 In particular, UCP-2 expression is increased (probably due to a condition of fuel overload), which leads to lower ATP, decreased ATP/ADP ratio, with consequent reduction of insulin release. Adenosine Triphosphate 129-132 uncoupling protein 2 Homo sapiens 15-20 15654602-9 2005 In particular, UCP-2 expression is increased (probably due to a condition of fuel overload), which leads to lower ATP, decreased ATP/ADP ratio, with consequent reduction of insulin release. Adenosine Diphosphate 133-136 uncoupling protein 2 Homo sapiens 15-20 15823721-4 2005 It is proposed that induction and activation of UCP2 may play a role in the thermogenesis evoked by hepatothermic therapy, a strategy designed to decrease body fat by maximizing hepatic fatty acid oxidation. Fatty Acids 186-196 uncoupling protein 2 Homo sapiens 48-52 16054055-3 2005 When human UCP2 (hUCP2) is targeted to the mitochondria of adult fly neurons, we find an increase in state 4 respiration, a decrease in ROS production, a decrease in oxidative damage, heightened resistance to the free radical generator paraquat, and an extension in life span without compromising fertility or physical activity. Reactive Oxygen Species 136-139 uncoupling protein 2 Homo sapiens 11-15 16054055-3 2005 When human UCP2 (hUCP2) is targeted to the mitochondria of adult fly neurons, we find an increase in state 4 respiration, a decrease in ROS production, a decrease in oxidative damage, heightened resistance to the free radical generator paraquat, and an extension in life span without compromising fertility or physical activity. Reactive Oxygen Species 136-139 uncoupling protein 2 Homo sapiens 17-22 15650415-0 2005 Selenium attenuates expression of MnSOD and uncoupling protein 2 in J774.2 macrophages: molecular mechanism for its cell-death and antiinflammatory activity. Selenium 0-8 uncoupling protein 2 Homo sapiens 44-64 15650415-13 2005 It is therefore concluded that selenium at high nanomolar to low micromolar concentrations shifts the balance between inflammatory response and cell death toward the latter, through a direct effect on the transcription factors Sp1 and NF-kappaB, and down-regulation of MnSOD and UCP2. Selenium 31-39 uncoupling protein 2 Homo sapiens 279-283 15599605-2 2005 This review focuses on transmitter-receptor mismatches in the brain, which is one of the hallmarks of the Volume Transmission (VT) concept, and how this phenomenon may be related to local temperature gradients created by brain uncoupling protein 2 (UCP2), which uncouples oxidative phosphorylation from ATP synthesis, hereby generating heat. Adenosine Triphosphate 303-306 uncoupling protein 2 Homo sapiens 227-247 15823721-2 2005 Under these circumstances, superoxide within the mitochondrial matrix directly activates uncoupling protein-2 (UCP2), and may also promote induction of this protein. Superoxides 27-37 uncoupling protein 2 Homo sapiens 89-109 15823721-2 2005 Under these circumstances, superoxide within the mitochondrial matrix directly activates uncoupling protein-2 (UCP2), and may also promote induction of this protein. Superoxides 27-37 uncoupling protein 2 Homo sapiens 111-115 15881342-0 2005 [The inhibiting effect of uncoupling protein 2 on the production of reactive oxygen species]. Reactive Oxygen Species 68-91 uncoupling protein 2 Homo sapiens 26-46 16075814-6 2005 The uncoupling protein-2 (UCP2) is found in many tissues and exerts dual effects: it protects cells function from damage caused by reactive oxygen species (ROS). Reactive Oxygen Species 131-154 uncoupling protein 2 Homo sapiens 4-24 16075814-6 2005 The uncoupling protein-2 (UCP2) is found in many tissues and exerts dual effects: it protects cells function from damage caused by reactive oxygen species (ROS). Reactive Oxygen Species 131-154 uncoupling protein 2 Homo sapiens 26-30 16075814-6 2005 The uncoupling protein-2 (UCP2) is found in many tissues and exerts dual effects: it protects cells function from damage caused by reactive oxygen species (ROS). Reactive Oxygen Species 156-159 uncoupling protein 2 Homo sapiens 4-24 16075814-6 2005 The uncoupling protein-2 (UCP2) is found in many tissues and exerts dual effects: it protects cells function from damage caused by reactive oxygen species (ROS). Reactive Oxygen Species 156-159 uncoupling protein 2 Homo sapiens 26-30 16075814-7 2005 On the other hand, the uncoupling induced by UCP2 in mitochondria of pancreatic beta cells decreases ATP synthesis and impairs insulin secretion in response to glucose. Adenosine Triphosphate 101-104 uncoupling protein 2 Homo sapiens 45-49 16075814-7 2005 On the other hand, the uncoupling induced by UCP2 in mitochondria of pancreatic beta cells decreases ATP synthesis and impairs insulin secretion in response to glucose. Glucose 160-167 uncoupling protein 2 Homo sapiens 45-49 15563984-0 2004 Enhancing effect of taurine on glucose response in UCP2-overexpressing beta cells. Taurine 20-27 uncoupling protein 2 Homo sapiens 51-55 15604077-5 2004 CONCLUSIONS: UCP2 down-regulates reactive oxygen species and plays a role in the regulation of inflammatory events to prepare for embryo implantation. Reactive Oxygen Species 33-56 uncoupling protein 2 Homo sapiens 13-17 15525551-4 2004 UCP2 and UCP3 appear to function in reactive oxygen species handling and/or in fatty acid metabolism; uncoupling might occur secondarily. Reactive Oxygen Species 36-59 uncoupling protein 2 Homo sapiens 0-4 15525551-4 2004 UCP2 and UCP3 appear to function in reactive oxygen species handling and/or in fatty acid metabolism; uncoupling might occur secondarily. Fatty Acids 79-89 uncoupling protein 2 Homo sapiens 0-4 15563984-0 2004 Enhancing effect of taurine on glucose response in UCP2-overexpressing beta cells. Glucose 31-38 uncoupling protein 2 Homo sapiens 51-55 15563984-1 2004 Uncoupling protein 2 (UCP2) is up-regulated in pancreatic beta cells when exposed to long-term high glucose or free fatty acids, which results in impaired glucose-induced insulin secretion (GIIS). Glucose 100-107 uncoupling protein 2 Homo sapiens 0-20 15563984-1 2004 Uncoupling protein 2 (UCP2) is up-regulated in pancreatic beta cells when exposed to long-term high glucose or free fatty acids, which results in impaired glucose-induced insulin secretion (GIIS). Glucose 100-107 uncoupling protein 2 Homo sapiens 22-26 15563984-1 2004 Uncoupling protein 2 (UCP2) is up-regulated in pancreatic beta cells when exposed to long-term high glucose or free fatty acids, which results in impaired glucose-induced insulin secretion (GIIS). Fatty Acids, Nonesterified 111-127 uncoupling protein 2 Homo sapiens 0-20 15563984-1 2004 Uncoupling protein 2 (UCP2) is up-regulated in pancreatic beta cells when exposed to long-term high glucose or free fatty acids, which results in impaired glucose-induced insulin secretion (GIIS). Fatty Acids, Nonesterified 111-127 uncoupling protein 2 Homo sapiens 22-26 15563984-2 2004 We have evaluated whether taurine pretreatment can restore impaired GIIS of beta cells overexpressing UCP2 by adenovirus (Ad)-mediated transfection technique. Taurine 26-33 uncoupling protein 2 Homo sapiens 102-106 15563984-6 2004 When taurine (3 mM) was pretreated for 24 h, the glucose responses of Ad-UCP2 cells were remarkably restored. Taurine 5-12 uncoupling protein 2 Homo sapiens 73-77 15563984-6 2004 When taurine (3 mM) was pretreated for 24 h, the glucose responses of Ad-UCP2 cells were remarkably restored. Glucose 49-56 uncoupling protein 2 Homo sapiens 73-77 15563984-8 2004 These results suggest that taurine restores impaired GIIS in Ad-UCP2 cells, at least partially, by acting on the mechanism for Ca2+ sequestration into the mitochondrial matrix. Taurine 27-34 uncoupling protein 2 Homo sapiens 64-68 15525581-0 2004 Overexpression of short heterodimer partner recovers impaired glucose-stimulated insulin secretion of pancreatic beta-cells overexpressing UCP2. Glucose 62-69 uncoupling protein 2 Homo sapiens 139-143 15322095-9 2004 In addition, we further demonstrate that the dissipation of DeltaPsim is accompanied by significant reduction of cellular ATP in 293T cells overexpressing HDMCP or uncoupling protein 2 (UCP2). Adenosine Triphosphate 122-125 uncoupling protein 2 Homo sapiens 164-184 15322095-9 2004 In addition, we further demonstrate that the dissipation of DeltaPsim is accompanied by significant reduction of cellular ATP in 293T cells overexpressing HDMCP or uncoupling protein 2 (UCP2). Adenosine Triphosphate 122-125 uncoupling protein 2 Homo sapiens 186-190 15541452-5 2004 When plasma free-fatty-acid concentrations were raised, cardiac mitochondrial uncoupling proteins (UCP) increased (isoform UCP2, p<0.0001; isoform UCP3, p=0.0036) and those of glucose transporter (GLUT4) protein decreased (cardiac, p=0.0001; skeletal muscle, p=0.0006). Fatty Acids 17-27 uncoupling protein 2 Homo sapiens 123-127 15525581-8 2004 SHP overexpression also corrected the impaired sensitivity of UCP2-overexpressing beta-cells to methylpyruvate, another energy fuel that bypasses glycolysis and directly enters the Krebs cycle. methyl pyruvate 96-110 uncoupling protein 2 Homo sapiens 62-66 15525581-8 2004 SHP overexpression also corrected the impaired sensitivity of UCP2-overexpressing beta-cells to methylpyruvate, another energy fuel that bypasses glycolysis and directly enters the Krebs cycle. krebs 181-186 uncoupling protein 2 Homo sapiens 62-66 15525581-11 2004 These results suggest that SHP positively regulates GSIS in beta-cells and restores glucose sensitivity in UCP2-overexpressing beta-cells by enhancing mitochondrial glucose metabolism, independent of PPARgamma activation. Glucose 84-91 uncoupling protein 2 Homo sapiens 107-111 15525581-11 2004 These results suggest that SHP positively regulates GSIS in beta-cells and restores glucose sensitivity in UCP2-overexpressing beta-cells by enhancing mitochondrial glucose metabolism, independent of PPARgamma activation. Glucose 165-172 uncoupling protein 2 Homo sapiens 107-111 15220218-1 2004 Uncoupling protein (UCP)-2 is a member of the mitochondrial inner membrane carriers that uncouple pro-ton entry in the mitochondrial matrix from ATP synthesis. Adenosine Triphosphate 145-148 uncoupling protein 2 Homo sapiens 0-26 15265758-0 2004 Taurine increases glucose sensitivity of UCP2-overexpressing beta-cells by ameliorating mitochondrial metabolism. Taurine 0-7 uncoupling protein 2 Homo sapiens 41-45 15265758-0 2004 Taurine increases glucose sensitivity of UCP2-overexpressing beta-cells by ameliorating mitochondrial metabolism. Glucose 18-25 uncoupling protein 2 Homo sapiens 41-45 15265758-6 2004 In freshly isolated mitochondria from UCP2-overexpressing insulin-secreting (INS)-1 beta-cells, methyl pyruvate-mediated mitochondrial Ca(2+) increase was significantly ameliorated by taurine. methyl pyruvate 96-111 uncoupling protein 2 Homo sapiens 38-42 15265758-6 2004 In freshly isolated mitochondria from UCP2-overexpressing insulin-secreting (INS)-1 beta-cells, methyl pyruvate-mediated mitochondrial Ca(2+) increase was significantly ameliorated by taurine. Taurine 184-191 uncoupling protein 2 Homo sapiens 38-42 15265758-8 2004 This study suggests that taurine enhances the glucose sensitivity of UCP2-overexpressing beta-cells, probably by increasing mitochondrial Ca(2+) influx through the Ca(2+) uniporter, thereby enhancing mitochondrial metabolic function and increasing the ATP/ADP ratio. Taurine 25-32 uncoupling protein 2 Homo sapiens 69-73 15265758-8 2004 This study suggests that taurine enhances the glucose sensitivity of UCP2-overexpressing beta-cells, probably by increasing mitochondrial Ca(2+) influx through the Ca(2+) uniporter, thereby enhancing mitochondrial metabolic function and increasing the ATP/ADP ratio. Glucose 46-53 uncoupling protein 2 Homo sapiens 69-73 15304252-3 2004 Superoxide and the lipid peroxidation products it engenders, including hydroxyalkenals such as hydroxynonenal, are potent activators of proton conductance by mitochondrial uncoupling proteins such as UCP2 and UCP3, although the mechanism of activation has yet to be established. Superoxides 0-10 uncoupling protein 2 Homo sapiens 200-204 15304252-3 2004 Superoxide and the lipid peroxidation products it engenders, including hydroxyalkenals such as hydroxynonenal, are potent activators of proton conductance by mitochondrial uncoupling proteins such as UCP2 and UCP3, although the mechanism of activation has yet to be established. hydroxyalkenals 71-86 uncoupling protein 2 Homo sapiens 200-204 15304252-3 2004 Superoxide and the lipid peroxidation products it engenders, including hydroxyalkenals such as hydroxynonenal, are potent activators of proton conductance by mitochondrial uncoupling proteins such as UCP2 and UCP3, although the mechanism of activation has yet to be established. hydroxynonenal 95-109 uncoupling protein 2 Homo sapiens 200-204 15448008-2 2004 One such mechanism may be via up-regulation of uncoupling protein-2 (UCP2), a mitochondrial inner membrane anion carrier recently found to provide cytoprotection in nontumor cells by acting as a sensor and negative regulator of reactive oxygen species production. Reactive Oxygen Species 228-251 uncoupling protein 2 Homo sapiens 47-67 15448008-2 2004 One such mechanism may be via up-regulation of uncoupling protein-2 (UCP2), a mitochondrial inner membrane anion carrier recently found to provide cytoprotection in nontumor cells by acting as a sensor and negative regulator of reactive oxygen species production. Reactive Oxygen Species 228-251 uncoupling protein 2 Homo sapiens 69-73 15448008-7 2004 RESULTS: UCP2 mRNA and protein levels were 3- to 4-fold higher in adenocarcinomas, and UCP2 mRNA levels showed significant correlation with increased tumor tissue malondialdehyde contents. Malondialdehyde 163-178 uncoupling protein 2 Homo sapiens 87-91 15534396-1 2004 The skeletal muscle mitochondria contain two isoforms of uncoupling protein, UCP2 and mainly UCP3, which had been shown to be activated by free fatty acids and inhibited by purine nucleotides in reconstituted systems. Fatty Acids, Nonesterified 139-155 uncoupling protein 2 Homo sapiens 77-81 15534396-1 2004 The skeletal muscle mitochondria contain two isoforms of uncoupling protein, UCP2 and mainly UCP3, which had been shown to be activated by free fatty acids and inhibited by purine nucleotides in reconstituted systems. Purine Nucleotides 173-191 uncoupling protein 2 Homo sapiens 77-81 15308133-7 2004 In conclusion, a common polymorphism in the promoter region of the UCP2 gene modulates triglycerides and cholesterol levels in French Caucasian subjects with type 2 diabetes. Triglycerides 87-100 uncoupling protein 2 Homo sapiens 67-71 15308133-7 2004 In conclusion, a common polymorphism in the promoter region of the UCP2 gene modulates triglycerides and cholesterol levels in French Caucasian subjects with type 2 diabetes. Cholesterol 105-116 uncoupling protein 2 Homo sapiens 67-71 15196910-0 2004 Links between fatty acids and expression of UCP2 and UCP3 mRNAs. Fatty Acids 14-25 uncoupling protein 2 Homo sapiens 44-48 15196910-4 2004 Oleic acid has been shown to stimulate the activity of the promoter regions of UCP2 and UCP3 genes and the FA responsive regions are beginning to be characterised. Oleic Acid 0-10 uncoupling protein 2 Homo sapiens 79-83 15196910-1 2004 Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. Fatty Acids 79-90 uncoupling protein 2 Homo sapiens 106-126 15196910-1 2004 Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. Fatty Acids 79-90 uncoupling protein 2 Homo sapiens 128-132 15196910-1 2004 Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. Fatty Acids 92-95 uncoupling protein 2 Homo sapiens 106-126 15196910-1 2004 Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. Fatty Acids 92-95 uncoupling protein 2 Homo sapiens 128-132 15016641-1 2004 OBJECTIVE: Uncoupling protein 2 (UCP2) belongs to the mitochondrial anion carrier family and regulates production of reactive oxygen species in macrophages. Reactive Oxygen Species 117-140 uncoupling protein 2 Homo sapiens 11-31 15016641-1 2004 OBJECTIVE: Uncoupling protein 2 (UCP2) belongs to the mitochondrial anion carrier family and regulates production of reactive oxygen species in macrophages. Reactive Oxygen Species 117-140 uncoupling protein 2 Homo sapiens 33-37 15016641-4 2004 METHODS AND RESULTS: UCP2 overexpression in THP1 monocytes, which induced a 10-fold increase in mitochondrial UCP2 protein levels, reduced steady-state level of intracellular reactive oxygen species (ROS) and H2O2-induced ROS production. Reactive Oxygen Species 175-198 uncoupling protein 2 Homo sapiens 21-25 15016641-4 2004 METHODS AND RESULTS: UCP2 overexpression in THP1 monocytes, which induced a 10-fold increase in mitochondrial UCP2 protein levels, reduced steady-state level of intracellular reactive oxygen species (ROS) and H2O2-induced ROS production. Reactive Oxygen Species 200-203 uncoupling protein 2 Homo sapiens 21-25 15016641-4 2004 METHODS AND RESULTS: UCP2 overexpression in THP1 monocytes, which induced a 10-fold increase in mitochondrial UCP2 protein levels, reduced steady-state level of intracellular reactive oxygen species (ROS) and H2O2-induced ROS production. Hydrogen Peroxide 209-213 uncoupling protein 2 Homo sapiens 21-25 15016641-4 2004 METHODS AND RESULTS: UCP2 overexpression in THP1 monocytes, which induced a 10-fold increase in mitochondrial UCP2 protein levels, reduced steady-state level of intracellular reactive oxygen species (ROS) and H2O2-induced ROS production. Reactive Oxygen Species 222-225 uncoupling protein 2 Homo sapiens 21-25 15016641-5 2004 THP1 monocytes with UCP2 overexpression showed lower intracellular calcium levels and less H2O2-triggered intracellular calcium mobilization, and less protein and mRNA levels of beta2 integrins, most notably CD11b. Calcium 67-74 uncoupling protein 2 Homo sapiens 20-24 15016641-5 2004 THP1 monocytes with UCP2 overexpression showed lower intracellular calcium levels and less H2O2-triggered intracellular calcium mobilization, and less protein and mRNA levels of beta2 integrins, most notably CD11b. Hydrogen Peroxide 91-95 uncoupling protein 2 Homo sapiens 20-24 15016641-5 2004 THP1 monocytes with UCP2 overexpression showed lower intracellular calcium levels and less H2O2-triggered intracellular calcium mobilization, and less protein and mRNA levels of beta2 integrins, most notably CD11b. Calcium 120-127 uncoupling protein 2 Homo sapiens 20-24 14679173-3 2003 A new study demonstrates that hyperglycemia-induced mitochondrial superoxide production activates uncoupling protein 2, which decreases the ATP/ADP ratio and thus reduces the insulin-secretory response. Superoxides 66-76 uncoupling protein 2 Homo sapiens 98-118 15163543-14 2004 Although without supportive data, superoxide production induced by arsenic exposure can theoretically impair insulin secretion by interaction with uncoupling protein 2 (UCP2), and oxidative stress can also cause amyloid formation in the pancreas, which could progressively destroy the insulin-secreting beta cells. Superoxides 34-44 uncoupling protein 2 Homo sapiens 147-167 15163543-14 2004 Although without supportive data, superoxide production induced by arsenic exposure can theoretically impair insulin secretion by interaction with uncoupling protein 2 (UCP2), and oxidative stress can also cause amyloid formation in the pancreas, which could progressively destroy the insulin-secreting beta cells. Superoxides 34-44 uncoupling protein 2 Homo sapiens 169-173 15163543-14 2004 Although without supportive data, superoxide production induced by arsenic exposure can theoretically impair insulin secretion by interaction with uncoupling protein 2 (UCP2), and oxidative stress can also cause amyloid formation in the pancreas, which could progressively destroy the insulin-secreting beta cells. Arsenic 67-74 uncoupling protein 2 Homo sapiens 147-167 15163543-14 2004 Although without supportive data, superoxide production induced by arsenic exposure can theoretically impair insulin secretion by interaction with uncoupling protein 2 (UCP2), and oxidative stress can also cause amyloid formation in the pancreas, which could progressively destroy the insulin-secreting beta cells. Arsenic 67-74 uncoupling protein 2 Homo sapiens 169-173 14749278-9 2004 In comparison to the established uncoupling and thermogenic activities of UCP1, UCP2 and UCP3 appear to be involved in the limitation of free radical levels in cells rather than in physiological uncoupling and thermogenesis. Free Radicals 137-149 uncoupling protein 2 Homo sapiens 80-84 15119950-6 2004 Functional activation of UCP2 is proposed to decrease reactive oxygen species (ROS) production. Reactive Oxygen Species 54-77 uncoupling protein 2 Homo sapiens 25-29 15119950-6 2004 Functional activation of UCP2 is proposed to decrease reactive oxygen species (ROS) production. Reactive Oxygen Species 79-82 uncoupling protein 2 Homo sapiens 25-29 15119950-7 2004 Moreover, reaction products of lipoperoxidation such as cleaved hydroperoxy-fatty acids and hydroxy-fatty acid can activate UCP2 and promote feedback down-regulation of mitochondrial ROS production. hydroperoxy-fatty acids 64-87 uncoupling protein 2 Homo sapiens 124-128 15119950-7 2004 Moreover, reaction products of lipoperoxidation such as cleaved hydroperoxy-fatty acids and hydroxy-fatty acid can activate UCP2 and promote feedback down-regulation of mitochondrial ROS production. hydroxy-fatty acid 92-110 uncoupling protein 2 Homo sapiens 124-128 15119950-7 2004 Moreover, reaction products of lipoperoxidation such as cleaved hydroperoxy-fatty acids and hydroxy-fatty acid can activate UCP2 and promote feedback down-regulation of mitochondrial ROS production. Reactive Oxygen Species 183-186 uncoupling protein 2 Homo sapiens 124-128 14679173-3 2003 A new study demonstrates that hyperglycemia-induced mitochondrial superoxide production activates uncoupling protein 2, which decreases the ATP/ADP ratio and thus reduces the insulin-secretory response. Adenosine Diphosphate 144-147 uncoupling protein 2 Homo sapiens 98-118 14679178-0 2003 Superoxide-mediated activation of uncoupling protein 2 causes pancreatic beta cell dysfunction. Superoxides 0-10 uncoupling protein 2 Homo sapiens 34-54 14679178-3 2003 Uncoupling protein 2 (UCP2), by virtue of its mitochondrial proton leak activity and consequent negative effect on ATP production, impairs glucose-stimulated insulin secretion. Adenosine Triphosphate 115-118 uncoupling protein 2 Homo sapiens 0-20 15039126-2 2004 Mitochondrial uncoupling protein 2 (UCP2) negatively regulates reactive oxygen species generation. Reactive Oxygen Species 63-86 uncoupling protein 2 Homo sapiens 0-34 15039126-2 2004 Mitochondrial uncoupling protein 2 (UCP2) negatively regulates reactive oxygen species generation. Reactive Oxygen Species 63-86 uncoupling protein 2 Homo sapiens 36-40 14693721-0 2004 The common -866 G/A polymorphism in the promoter of uncoupling protein 2 is associated with increased carbohydrate and decreased lipid oxidation in juvenile obesity. Carbohydrates 102-114 uncoupling protein 2 Homo sapiens 52-72 14693721-1 2004 Uncoupling protein (UCP) 2 is a member of the mitochondrial transporter superfamily that uncouples proton entry in the mitochondrial matrix from ATP synthesis. Adenosine Triphosphate 145-148 uncoupling protein 2 Homo sapiens 0-26 14693721-6 2004 Metabolic studies in 147 of these juvenile obese subjects showed that homozygosity for the UCP2 promoter variant A was associated with important changes in energy metabolism compared with other genotypes, i.e., a 34% increase of carbohydrate oxidation (94 +/- 10 vs. 70 +/- 3 mg.min(-1).m(-2), P = 0.004) and a 23% decrease of lipid oxidation (26 +/- 3 vs. 34 +/- 1 mg.min(-1).m(-2), P = 0.03). Carbohydrates 229-241 uncoupling protein 2 Homo sapiens 91-95 14679178-3 2003 Uncoupling protein 2 (UCP2), by virtue of its mitochondrial proton leak activity and consequent negative effect on ATP production, impairs glucose-stimulated insulin secretion. Adenosine Triphosphate 115-118 uncoupling protein 2 Homo sapiens 22-26 14679178-3 2003 Uncoupling protein 2 (UCP2), by virtue of its mitochondrial proton leak activity and consequent negative effect on ATP production, impairs glucose-stimulated insulin secretion. Glucose 139-146 uncoupling protein 2 Homo sapiens 0-20 14679178-3 2003 Uncoupling protein 2 (UCP2), by virtue of its mitochondrial proton leak activity and consequent negative effect on ATP production, impairs glucose-stimulated insulin secretion. Glucose 139-146 uncoupling protein 2 Homo sapiens 22-26 14679178-4 2003 Of interest, it has recently been shown that superoxide, when added to isolated mitochondria, activates UCP2-mediated proton leak. Superoxides 45-55 uncoupling protein 2 Homo sapiens 104-108 14679178-5 2003 Since obesity and chronic hyperglycemia increase mitochondrial superoxide production, as well as UCP2 expression in pancreatic beta cells, a superoxide-UCP2 pathway could contribute importantly to obesity- and hyperglycemia-induced beta cell dysfunction. Superoxides 63-73 uncoupling protein 2 Homo sapiens 152-156 14679178-5 2003 Since obesity and chronic hyperglycemia increase mitochondrial superoxide production, as well as UCP2 expression in pancreatic beta cells, a superoxide-UCP2 pathway could contribute importantly to obesity- and hyperglycemia-induced beta cell dysfunction. Superoxides 141-151 uncoupling protein 2 Homo sapiens 97-101 14679178-5 2003 Since obesity and chronic hyperglycemia increase mitochondrial superoxide production, as well as UCP2 expression in pancreatic beta cells, a superoxide-UCP2 pathway could contribute importantly to obesity- and hyperglycemia-induced beta cell dysfunction. Superoxides 141-151 uncoupling protein 2 Homo sapiens 152-156 14679173-3 2003 A new study demonstrates that hyperglycemia-induced mitochondrial superoxide production activates uncoupling protein 2, which decreases the ATP/ADP ratio and thus reduces the insulin-secretory response. Adenosine Triphosphate 140-143 uncoupling protein 2 Homo sapiens 98-118 14679178-6 2003 This study demonstrates that endogenously produced mitochondrial superoxide activates UCP2-mediated proton leak, thus lowering ATP levels and impairing glucose-stimulated insulin secretion. Superoxides 65-75 uncoupling protein 2 Homo sapiens 86-90 14679178-6 2003 This study demonstrates that endogenously produced mitochondrial superoxide activates UCP2-mediated proton leak, thus lowering ATP levels and impairing glucose-stimulated insulin secretion. Adenosine Triphosphate 127-130 uncoupling protein 2 Homo sapiens 86-90 14740889-4 2003 N-methylanthraniloyl-tagged ATP (Mant-ATP) experiments indicated two independent inclusion body UCP2 binding sites with dissociation constants (Kd) of 0.3-0.5 and 23-92 microM. n-methylanthraniloyl 0-20 uncoupling protein 2 Homo sapiens 96-100 14679178-6 2003 This study demonstrates that endogenously produced mitochondrial superoxide activates UCP2-mediated proton leak, thus lowering ATP levels and impairing glucose-stimulated insulin secretion. Glucose 152-159 uncoupling protein 2 Homo sapiens 86-90 14679178-9 2003 Therefore, superoxide-mediated activation of UCP2 could play an important role in the pathogenesis of beta cell dysfunction and type 2 diabetes. Superoxides 11-21 uncoupling protein 2 Homo sapiens 45-49 14642350-5 2003 Combined H(2)O(2) and glucagon treatment, but not H(2)O(2) or glucagon used alone, increased the hepatocyte oxygen uptake rate 25% above control untreated cells after a lag-time of 72 h. The same treatment also increased the expression of mitochondrial uncoupling protein-2 (UCP2). Hydrogen Peroxide 9-17 uncoupling protein 2 Homo sapiens 239-273 14642350-5 2003 Combined H(2)O(2) and glucagon treatment, but not H(2)O(2) or glucagon used alone, increased the hepatocyte oxygen uptake rate 25% above control untreated cells after a lag-time of 72 h. The same treatment also increased the expression of mitochondrial uncoupling protein-2 (UCP2). Hydrogen Peroxide 9-17 uncoupling protein 2 Homo sapiens 275-279 14642350-5 2003 Combined H(2)O(2) and glucagon treatment, but not H(2)O(2) or glucagon used alone, increased the hepatocyte oxygen uptake rate 25% above control untreated cells after a lag-time of 72 h. The same treatment also increased the expression of mitochondrial uncoupling protein-2 (UCP2). Glucagon 22-30 uncoupling protein 2 Homo sapiens 239-273 14642350-5 2003 Combined H(2)O(2) and glucagon treatment, but not H(2)O(2) or glucagon used alone, increased the hepatocyte oxygen uptake rate 25% above control untreated cells after a lag-time of 72 h. The same treatment also increased the expression of mitochondrial uncoupling protein-2 (UCP2). Glucagon 22-30 uncoupling protein 2 Homo sapiens 275-279 14642350-8 2003 These findings show, for the first time, that oxidative stress, in combination with glucagon, increases metabolic energy expenditure in cultured cells, and that this effect may be mediated by UCP-2. Glucagon 84-92 uncoupling protein 2 Homo sapiens 192-197 14740889-6 2003 By direct titration, UCP2 bound [8-(14)C] ATP and [8-(14)C] ADP with Kds of 4-5 and 16-18 microM, respectively. [8-(14)c] adp 50-63 uncoupling protein 2 Homo sapiens 21-25 14740889-8 2003 A combination of gel filtration, Cu2+-phenanthroline cross-linking, and ultracentrifugation indicated that 75-80% of UCP2 was in a monodisperse, 197 kDa form while the remainder was aggregated. cu2+-phenanthroline 33-52 uncoupling protein 2 Homo sapiens 117-121 14740889-9 2003 We conclude that (a) Mant-tagged nucleotides are useful fluorescent probes with isolated UCP2 when used with dimethylanthranilate controls; (b) UCP2 binds Mg2+-free nucleotides: the Kd for ATP is about 3-5 microM and for Mant-ATP it is about 10 times lower; and (c) in C12E9 detergent, the monodisperse protein may be in dimeric form. allyl p-tolyl ether 109-129 uncoupling protein 2 Homo sapiens 144-148 14740889-9 2003 We conclude that (a) Mant-tagged nucleotides are useful fluorescent probes with isolated UCP2 when used with dimethylanthranilate controls; (b) UCP2 binds Mg2+-free nucleotides: the Kd for ATP is about 3-5 microM and for Mant-ATP it is about 10 times lower; and (c) in C12E9 detergent, the monodisperse protein may be in dimeric form. magnesium ion 155-159 uncoupling protein 2 Homo sapiens 144-148 14740889-9 2003 We conclude that (a) Mant-tagged nucleotides are useful fluorescent probes with isolated UCP2 when used with dimethylanthranilate controls; (b) UCP2 binds Mg2+-free nucleotides: the Kd for ATP is about 3-5 microM and for Mant-ATP it is about 10 times lower; and (c) in C12E9 detergent, the monodisperse protein may be in dimeric form. mant 21-25 uncoupling protein 2 Homo sapiens 89-93 14740889-9 2003 We conclude that (a) Mant-tagged nucleotides are useful fluorescent probes with isolated UCP2 when used with dimethylanthranilate controls; (b) UCP2 binds Mg2+-free nucleotides: the Kd for ATP is about 3-5 microM and for Mant-ATP it is about 10 times lower; and (c) in C12E9 detergent, the monodisperse protein may be in dimeric form. mant 21-25 uncoupling protein 2 Homo sapiens 144-148 14518702-9 2003 CONCLUSION: Differential effects of stavudine and zidovudine therapy on mtDNA depletion and expression of adipocyte differentiation markers PPARgamma and UCP2 were observed, consistent with increased adipose tissue toxicity associated with stavudine therapy. Stavudine 36-45 uncoupling protein 2 Homo sapiens 154-158 12855674-3 2003 In the present study, we tested the hypothesis that UCP2 overexpression could protect cardiomyocytes from oxidative stress-induced cell death by reducing reactive oxygen species (ROS) production in mitochondria. Reactive Oxygen Species 154-177 uncoupling protein 2 Homo sapiens 52-56 12855674-3 2003 In the present study, we tested the hypothesis that UCP2 overexpression could protect cardiomyocytes from oxidative stress-induced cell death by reducing reactive oxygen species (ROS) production in mitochondria. Reactive Oxygen Species 179-182 uncoupling protein 2 Homo sapiens 52-56 12855674-5 2003 UCP2 overexpression significantly suppressed markers of cell death, including TUNEL positivity, phosphatidylserine exposure, propidium iodide uptake, and caspase-3 cleavage. Phosphatidylserines 96-114 uncoupling protein 2 Homo sapiens 0-4 12855674-5 2003 UCP2 overexpression significantly suppressed markers of cell death, including TUNEL positivity, phosphatidylserine exposure, propidium iodide uptake, and caspase-3 cleavage. Propidium 125-141 uncoupling protein 2 Homo sapiens 0-4 12855674-6 2003 Furthermore, UCP2 remarkably prevented the catastrophic loss of mitochondrial inner membrane potential induced by H2O2, which is a critical early event in cell death. Hydrogen Peroxide 114-118 uncoupling protein 2 Homo sapiens 13-17 12855674-7 2003 Ca2+ overload and the production of ROS in mitochondria, both of which contribute to mitochondrial inner membrane potential loss, were dramatically attenuated by UCP2 overexpression. Reactive Oxygen Species 36-39 uncoupling protein 2 Homo sapiens 162-166 12855674-8 2003 Thus, overexpression of UCP2 attenuates ROS generation and prevents mitochondrial Ca2+ overload, revealing a novel mechanism of cardioprotection. Reactive Oxygen Species 40-43 uncoupling protein 2 Homo sapiens 24-28 14518702-9 2003 CONCLUSION: Differential effects of stavudine and zidovudine therapy on mtDNA depletion and expression of adipocyte differentiation markers PPARgamma and UCP2 were observed, consistent with increased adipose tissue toxicity associated with stavudine therapy. Zidovudine 50-60 uncoupling protein 2 Homo sapiens 154-158 12858170-4 2003 In cultured cortical neurons, UCP-2 reduced cell death and inhibited caspase-3 activation induced by oxygen and glucose deprivation. Oxygen 101-107 uncoupling protein 2 Homo sapiens 30-35 12858170-5 2003 Mild mitochondrial uncoupling by 2,4-dinitrophenol (DNP) reduced neuronal death, and UCP-2 activity was enhanced by palmitic acid in isolated mitochondria. Palmitic Acid 116-129 uncoupling protein 2 Homo sapiens 85-90 12858170-6 2003 Also in isolated mitochondria, UCP-2 shifted the release of reactive oxygen species from the mitochondrial matrix to the extramitochondrial space. Reactive Oxygen Species 60-83 uncoupling protein 2 Homo sapiens 31-36 12734183-4 2003 The Ki values for ATP inhibition were 50 microm (UCP1), 70 microm (UCP2), and 120 microm (UCP3) at pH 7.2. Adenosine Triphosphate 18-21 uncoupling protein 2 Homo sapiens 67-71 12797456-1 2003 BACKGROUND: Polymorphisms in the mitochondrial membrane transporter gene UCP2 are capable of affecting energy metabolism, body weight regulation, and possibly preventing the buildup of reactive oxygen species, all factors that could contribute to neural tube defect risk through maternal obesity and diabetes. Reactive Oxygen Species 185-208 uncoupling protein 2 Homo sapiens 73-77 12864746-9 2003 By targeting the UCP2-gene there was no effect on whole body energy metabolism, but instead, a reduced ability to protect against free-radical oxygen species. Oxygen 143-149 uncoupling protein 2 Homo sapiens 17-21 12716765-0 2003 A common polymorphism in the promoter of UCP2 contributes to the variation in insulin secretion in glucose-tolerant subjects. Glucose 99-106 uncoupling protein 2 Homo sapiens 41-45 12637982-0 2003 Thiazolidinediones (PPARgamma ligands) increase IRS-1, UCP-2 and C/EBPalpha expression, but not transdifferentiation, in L6 muscle cells. Thiazolidinediones 0-18 uncoupling protein 2 Homo sapiens 55-60 12223452-6 2002 In contrast, nuclear vitamin D receptor (nVDR) knockout via antisense oligodeoxynucleotide (ODN) prevented the inhibitory effect of 1alpha,25-(OH)2-D3 on adipocyte UCP2 expression and protein levels. Oligodeoxyribonucleotides 70-90 uncoupling protein 2 Homo sapiens 164-168 12401727-4 2002 On the other hand, studies in animal and cell culture models identified pancreatic beta-cell UCP2 expression as a main determinant of the insulin secretory response to glucose. Glucose 168-175 uncoupling protein 2 Homo sapiens 93-97 12588051-0 2002 Regulation of uncoupling protein-2 mRNA in L6 myotubules: I: Thiazolidinediones stimulate uncoupling protein-2 gene expression by a mechanism requiring ongoing protein synthesis and an active mitogen-activated protein kinase. Thiazolidinediones 61-79 uncoupling protein 2 Homo sapiens 14-34 12588051-0 2002 Regulation of uncoupling protein-2 mRNA in L6 myotubules: I: Thiazolidinediones stimulate uncoupling protein-2 gene expression by a mechanism requiring ongoing protein synthesis and an active mitogen-activated protein kinase. Thiazolidinediones 61-79 uncoupling protein 2 Homo sapiens 90-110 12588051-2 2002 It has been reported that the insulin sensitizers, thiazolidinediones (TZDs), increase UCP2 mRNA levels and, more recently, that TZDs stimulate UCP2 reporter genes but that the sequences involved do not bind peroxisome proliferator-activated receptor gamma (PPARgamma). Thiazolidinediones 51-69 uncoupling protein 2 Homo sapiens 87-91 12588051-2 2002 It has been reported that the insulin sensitizers, thiazolidinediones (TZDs), increase UCP2 mRNA levels and, more recently, that TZDs stimulate UCP2 reporter genes but that the sequences involved do not bind peroxisome proliferator-activated receptor gamma (PPARgamma). Thiazolidinediones 51-69 uncoupling protein 2 Homo sapiens 144-148 12223452-0 2002 1alpha,25-dihydroxyvitamin D3 inhibits uncoupling protein 2 expression in human adipocytes. Calcitriol 0-29 uncoupling protein 2 Homo sapiens 39-59 12223452-3 2002 Accordingly, we have evaluated the role of 1alpha,25-(OH)2-D3 in regulating human adipocyte UCP2 expression. 25-(oh)2-d3 50-61 uncoupling protein 2 Homo sapiens 92-96 12223452-4 2002 Treatment of human adipocytes for 48 h with 1 nM 1alpha,25-(OH)2-D3 inhibited UCP2 mRNA and protein levels by 50% (P<0.002) and completely blocked isoproterenol- or fatty acid-stimulated two- to threefold increases in UCP2 expression. 1alpha 49-55 uncoupling protein 2 Homo sapiens 78-82 12223452-4 2002 Treatment of human adipocytes for 48 h with 1 nM 1alpha,25-(OH)2-D3 inhibited UCP2 mRNA and protein levels by 50% (P<0.002) and completely blocked isoproterenol- or fatty acid-stimulated two- to threefold increases in UCP2 expression. 1alpha 49-55 uncoupling protein 2 Homo sapiens 221-225 12223452-4 2002 Treatment of human adipocytes for 48 h with 1 nM 1alpha,25-(OH)2-D3 inhibited UCP2 mRNA and protein levels by 50% (P<0.002) and completely blocked isoproterenol- or fatty acid-stimulated two- to threefold increases in UCP2 expression. 25-(oh)2-d3 56-67 uncoupling protein 2 Homo sapiens 78-82 12223452-4 2002 Treatment of human adipocytes for 48 h with 1 nM 1alpha,25-(OH)2-D3 inhibited UCP2 mRNA and protein levels by 50% (P<0.002) and completely blocked isoproterenol- or fatty acid-stimulated two- to threefold increases in UCP2 expression. 25-(oh)2-d3 56-67 uncoupling protein 2 Homo sapiens 221-225 12196511-9 2002 However, it could play a role in preventing reactive oxygen species production as proposed for mammalian UCP2 and UCP3. Reactive Oxygen Species 44-67 uncoupling protein 2 Homo sapiens 105-109 12475182-2 2002 In the present work, we aimed to investigate the effects of the main physiological male and female sex hormones, i.e. testosterone, progesterone and 17-beta-estradiol, on the expression of uncoupling protein I (UCP1)--the main mediator of BAT thermogenesis--and on UCP2 and lipid accumulation in rodent brown adipocytes differentiated in culture. Testosterone 118-130 uncoupling protein 2 Homo sapiens 265-269 12475182-2 2002 In the present work, we aimed to investigate the effects of the main physiological male and female sex hormones, i.e. testosterone, progesterone and 17-beta-estradiol, on the expression of uncoupling protein I (UCP1)--the main mediator of BAT thermogenesis--and on UCP2 and lipid accumulation in rodent brown adipocytes differentiated in culture. Progesterone 132-144 uncoupling protein 2 Homo sapiens 265-269 12475182-2 2002 In the present work, we aimed to investigate the effects of the main physiological male and female sex hormones, i.e. testosterone, progesterone and 17-beta-estradiol, on the expression of uncoupling protein I (UCP1)--the main mediator of BAT thermogenesis--and on UCP2 and lipid accumulation in rodent brown adipocytes differentiated in culture. Estradiol 149-166 uncoupling protein 2 Homo sapiens 265-269 12475182-7 2002 Interestingly, the specific progesterone receptor antagonist RU486 induced UCP1 and UCP2 mRNAs, including UCP1 mRNA expression in non-NE-treated brown adipocytes, suggesting a profound effect of this antiprogestagen on brown adipocyte thermogenic capacity. Mifepristone 61-66 uncoupling protein 2 Homo sapiens 84-88 12475182-8 2002 Thus, are conclude that testosterone, 17-beta-estradiol, progesterone and RU486 have distinct actions on brown adipocytes, thus modulating UCP1 and UCP2 mRNA expression and/or lipid accumulation, and that sex hormones are factors that may explain in part the gender-dependent BAT thermogenic response. Testosterone 24-36 uncoupling protein 2 Homo sapiens 148-152 12475182-8 2002 Thus, are conclude that testosterone, 17-beta-estradiol, progesterone and RU486 have distinct actions on brown adipocytes, thus modulating UCP1 and UCP2 mRNA expression and/or lipid accumulation, and that sex hormones are factors that may explain in part the gender-dependent BAT thermogenic response. Estradiol 38-55 uncoupling protein 2 Homo sapiens 148-152 12475182-8 2002 Thus, are conclude that testosterone, 17-beta-estradiol, progesterone and RU486 have distinct actions on brown adipocytes, thus modulating UCP1 and UCP2 mRNA expression and/or lipid accumulation, and that sex hormones are factors that may explain in part the gender-dependent BAT thermogenic response. Progesterone 57-69 uncoupling protein 2 Homo sapiens 148-152 12475182-8 2002 Thus, are conclude that testosterone, 17-beta-estradiol, progesterone and RU486 have distinct actions on brown adipocytes, thus modulating UCP1 and UCP2 mRNA expression and/or lipid accumulation, and that sex hormones are factors that may explain in part the gender-dependent BAT thermogenic response. Mifepristone 74-79 uncoupling protein 2 Homo sapiens 148-152 12030845-2 2002 For this, sarkosyl-solubilized UCP2 inclusion bodies were treated with the polyoxyethylene ether detergent C12E9 and hydroxyapatite. sarkosyl 10-18 uncoupling protein 2 Homo sapiens 31-35 12030845-2 2002 For this, sarkosyl-solubilized UCP2 inclusion bodies were treated with the polyoxyethylene ether detergent C12E9 and hydroxyapatite. Polyethylene Glycols 75-90 uncoupling protein 2 Homo sapiens 31-35 12030845-2 2002 For this, sarkosyl-solubilized UCP2 inclusion bodies were treated with the polyoxyethylene ether detergent C12E9 and hydroxyapatite. Durapatite 117-131 uncoupling protein 2 Homo sapiens 31-35 12030845-4 2002 90% pure UCP2, as judged by Coomassie Blue and silver staining of polyacrylamide gels. Coomassie blue 28-42 uncoupling protein 2 Homo sapiens 9-13 12030845-4 2002 90% pure UCP2, as judged by Coomassie Blue and silver staining of polyacrylamide gels. polyacrylamide 66-80 uncoupling protein 2 Homo sapiens 9-13 12030845-5 2002 Using fluorescence resonance energy transfer, N-methylanthraniloyl-tagged purine nucleoside di- and tri-phosphates exhibited enhanced fluorescence with purified UCP2. -methylanthraniloyl 47-66 uncoupling protein 2 Homo sapiens 161-165 12030845-5 2002 Using fluorescence resonance energy transfer, N-methylanthraniloyl-tagged purine nucleoside di- and tri-phosphates exhibited enhanced fluorescence with purified UCP2. purine 74-80 uncoupling protein 2 Homo sapiens 161-165 12153598-9 2002 Furthermore, exposure of human myocytes to FFA for 24 h strongly induced both UCP3 and peroxisome proliferator-activated receptor-gamma (PPARgamma) but not UCP2 gene expression. Fatty Acids, Nonesterified 43-46 uncoupling protein 2 Homo sapiens 156-160 12445169-2 2002 Because uncoupling protein-2 is expressed in liver and reactive oxygen species are involved in pathogenesis of various liver diseases, this protein may protect liver cells from disease-associated oxidative stress. Reactive Oxygen Species 55-78 uncoupling protein 2 Homo sapiens 8-28 12445169-8 2002 The percentage of uncoupling protein-2 positive bile ducts in primary biliary cirrhosis patients treated with ursodeoxycholic acid was significantly lower than in untreated patients. Ursodeoxycholic Acid 110-130 uncoupling protein 2 Homo sapiens 18-38 12588051-2 2002 It has been reported that the insulin sensitizers, thiazolidinediones (TZDs), increase UCP2 mRNA levels and, more recently, that TZDs stimulate UCP2 reporter genes but that the sequences involved do not bind peroxisome proliferator-activated receptor gamma (PPARgamma). Thiazolidinediones 71-75 uncoupling protein 2 Homo sapiens 87-91 12588051-2 2002 It has been reported that the insulin sensitizers, thiazolidinediones (TZDs), increase UCP2 mRNA levels and, more recently, that TZDs stimulate UCP2 reporter genes but that the sequences involved do not bind peroxisome proliferator-activated receptor gamma (PPARgamma). Thiazolidinediones 71-75 uncoupling protein 2 Homo sapiens 144-148 12588051-2 2002 It has been reported that the insulin sensitizers, thiazolidinediones (TZDs), increase UCP2 mRNA levels and, more recently, that TZDs stimulate UCP2 reporter genes but that the sequences involved do not bind peroxisome proliferator-activated receptor gamma (PPARgamma). Thiazolidinediones 129-133 uncoupling protein 2 Homo sapiens 144-148 12588051-3 2002 We report here that TZDs stimulated UCP2 gene (ucp2) transcription in L6 myotubules involving an indirect mechanism. Thiazolidinediones 20-24 uncoupling protein 2 Homo sapiens 36-40 12588051-3 2002 We report here that TZDs stimulated UCP2 gene (ucp2) transcription in L6 myotubules involving an indirect mechanism. Thiazolidinediones 20-24 uncoupling protein 2 Homo sapiens 47-51 12588051-5 2002 UCP2 mRNA levels were increased in a time- and concentration-dependent manner by TZDs. Thiazolidinediones 81-85 uncoupling protein 2 Homo sapiens 0-4 12588051-7 2002 Bisphenol A diglycidyl ether, a PPARy antagonist, concentration dependently inhibited the TZD-induced increase in UCP2 mRNA. bisphenol A 0-11 uncoupling protein 2 Homo sapiens 114-118 12588051-7 2002 Bisphenol A diglycidyl ether, a PPARy antagonist, concentration dependently inhibited the TZD-induced increase in UCP2 mRNA. DIGLYCIDYL ETHER 12-28 uncoupling protein 2 Homo sapiens 114-118 12588051-7 2002 Bisphenol A diglycidyl ether, a PPARy antagonist, concentration dependently inhibited the TZD-induced increase in UCP2 mRNA. 2,4-thiazolidinedione 90-93 uncoupling protein 2 Homo sapiens 114-118 12588051-8 2002 Blockade of protein synthesis with cycloheximide as well as abrogation of mitogen-activated protein kinase (MAPK) activity with PD98059 or U0126 also prevented the TZD-induced increase in UCP2 mRNA. Cycloheximide 35-48 uncoupling protein 2 Homo sapiens 188-192 12588051-8 2002 Blockade of protein synthesis with cycloheximide as well as abrogation of mitogen-activated protein kinase (MAPK) activity with PD98059 or U0126 also prevented the TZD-induced increase in UCP2 mRNA. 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one 128-135 uncoupling protein 2 Homo sapiens 188-192 12588051-8 2002 Blockade of protein synthesis with cycloheximide as well as abrogation of mitogen-activated protein kinase (MAPK) activity with PD98059 or U0126 also prevented the TZD-induced increase in UCP2 mRNA. U 0126 139-144 uncoupling protein 2 Homo sapiens 188-192 12588051-8 2002 Blockade of protein synthesis with cycloheximide as well as abrogation of mitogen-activated protein kinase (MAPK) activity with PD98059 or U0126 also prevented the TZD-induced increase in UCP2 mRNA. 2,4-thiazolidinedione 164-167 uncoupling protein 2 Homo sapiens 188-192 12588051-9 2002 As with autologous UCP2 mRNA, TZDs stimulated reporter gene expression directed by ucp2 sequences in transiently transfected L6 cells. Thiazolidinediones 30-34 uncoupling protein 2 Homo sapiens 19-23 12588051-9 2002 As with autologous UCP2 mRNA, TZDs stimulated reporter gene expression directed by ucp2 sequences in transiently transfected L6 cells. Thiazolidinediones 30-34 uncoupling protein 2 Homo sapiens 83-87 12588051-11 2002 TZDs, however, did not increase the activation of MAPK, nor did its activation by other means (change of medium, insulin-like growth factor-1, insulin) increase UCP2 mRNA, indicating that phosphorylation is not limiting. Thiazolidinediones 0-4 uncoupling protein 2 Homo sapiens 161-165 12588051-12 2002 These results suggest that TZDs indirectly stimulate ucp2 transcription by inducing-via PPARgamma-limiting amounts of a protein, which must be phosphorylated by MAPK to stimulate the gene. Thiazolidinediones 27-31 uncoupling protein 2 Homo sapiens 53-57 12588052-0 2002 Regulation of uncoupling protein-2 mRNA in L6 myotubules: II: Thyroid hormone amplifies stimulation of uncoupling protein-2 gene by thiazolidinediones and other peroxisome proliferator-activated receptor ligands in L6 myotubules: evidence for a priming effect. Thiazolidinediones 132-150 uncoupling protein 2 Homo sapiens 14-34 12588052-0 2002 Regulation of uncoupling protein-2 mRNA in L6 myotubules: II: Thyroid hormone amplifies stimulation of uncoupling protein-2 gene by thiazolidinediones and other peroxisome proliferator-activated receptor ligands in L6 myotubules: evidence for a priming effect. Thiazolidinediones 132-150 uncoupling protein 2 Homo sapiens 103-123 12588052-1 2002 The stimulation of the uncoupling protein-2 gene (ucp2) by thyroid hormone (triiodothyronine [T3]) in vivo is variable, suggesting complex interactions and even the possibility of indirect effects. Triiodothyronine 76-92 uncoupling protein 2 Homo sapiens 23-43 12588052-1 2002 The stimulation of the uncoupling protein-2 gene (ucp2) by thyroid hormone (triiodothyronine [T3]) in vivo is variable, suggesting complex interactions and even the possibility of indirect effects. Triiodothyronine 76-92 uncoupling protein 2 Homo sapiens 50-54 12588052-1 2002 The stimulation of the uncoupling protein-2 gene (ucp2) by thyroid hormone (triiodothyronine [T3]) in vivo is variable, suggesting complex interactions and even the possibility of indirect effects. Triiodothyronine 94-96 uncoupling protein 2 Homo sapiens 23-43 12588052-1 2002 The stimulation of the uncoupling protein-2 gene (ucp2) by thyroid hormone (triiodothyronine [T3]) in vivo is variable, suggesting complex interactions and even the possibility of indirect effects. Triiodothyronine 94-96 uncoupling protein 2 Homo sapiens 50-54 12588052-5 2002 T3 also enhanced the stimulation of ucp2 by the nonselective peroxisome proliferator-activated receptor (PPAR) ligands bezafibrate and carbacyclin, but not that by oleic acid or norepinephrine. carboprostacyclin 135-146 uncoupling protein 2 Homo sapiens 36-40 12588052-10 2002 The histone deacetylases inhibitor trichostatin A (TSA) stimulated the expression of ucp2 but did not add to the effect of T3 nor did this hormone enhance the effect of TSA. trichostatin A 35-49 uncoupling protein 2 Homo sapiens 85-89 12588052-10 2002 The histone deacetylases inhibitor trichostatin A (TSA) stimulated the expression of ucp2 but did not add to the effect of T3 nor did this hormone enhance the effect of TSA. trichostatin A 51-54 uncoupling protein 2 Homo sapiens 85-89 12588052-11 2002 These results suggest that T3 selectively enhances the transcriptional stimulation of ucp2 by TZDs and nonselective PPAR ligands by priming the gene to a transactivating signal(s) generated by such ligands. Thiazolidinediones 94-98 uncoupling protein 2 Homo sapiens 86-90 12030845-5 2002 Using fluorescence resonance energy transfer, N-methylanthraniloyl-tagged purine nucleoside di- and tri-phosphates exhibited enhanced fluorescence with purified UCP2. nucleoside di- and tri-phosphates 81-114 uncoupling protein 2 Homo sapiens 161-165 12030845-7 2002 Competition experiments with [8-14C]ATP demonstrated that UCP2 binds unmodified purine and pyrimidine nucleoside triphosphates with 2-5 microM affinity. [8-14c]atp 29-39 uncoupling protein 2 Homo sapiens 58-62 12030845-7 2002 Competition experiments with [8-14C]ATP demonstrated that UCP2 binds unmodified purine and pyrimidine nucleoside triphosphates with 2-5 microM affinity. purine 80-86 uncoupling protein 2 Homo sapiens 58-62 12030845-7 2002 Competition experiments with [8-14C]ATP demonstrated that UCP2 binds unmodified purine and pyrimidine nucleoside triphosphates with 2-5 microM affinity. pyrimidine nucleoside triphosphates 91-126 uncoupling protein 2 Homo sapiens 58-62 12030845-10 2002 These data indicate that: UCP2 (a) is at least partially refolded from sarkosyl-solubilized bacterial inclusion bodies by a two-step treatment with C12E9 detergent and hydroxyapatite; (b) binds purine and pyrimidine nucleoside triphosphates with low micromolar affinity; (c) binds GDP with the same affinity as GDP inhibits superoxide-stimulated uncoupling by kidney mitochondria; and (d) exhibits a different nucleotide preference than kidney mitochondria. sarkosyl 71-79 uncoupling protein 2 Homo sapiens 26-30 12030845-10 2002 These data indicate that: UCP2 (a) is at least partially refolded from sarkosyl-solubilized bacterial inclusion bodies by a two-step treatment with C12E9 detergent and hydroxyapatite; (b) binds purine and pyrimidine nucleoside triphosphates with low micromolar affinity; (c) binds GDP with the same affinity as GDP inhibits superoxide-stimulated uncoupling by kidney mitochondria; and (d) exhibits a different nucleotide preference than kidney mitochondria. purine 194-200 uncoupling protein 2 Homo sapiens 26-30 12030845-10 2002 These data indicate that: UCP2 (a) is at least partially refolded from sarkosyl-solubilized bacterial inclusion bodies by a two-step treatment with C12E9 detergent and hydroxyapatite; (b) binds purine and pyrimidine nucleoside triphosphates with low micromolar affinity; (c) binds GDP with the same affinity as GDP inhibits superoxide-stimulated uncoupling by kidney mitochondria; and (d) exhibits a different nucleotide preference than kidney mitochondria. pyrimidine nucleoside triphosphates 205-240 uncoupling protein 2 Homo sapiens 26-30 12030845-10 2002 These data indicate that: UCP2 (a) is at least partially refolded from sarkosyl-solubilized bacterial inclusion bodies by a two-step treatment with C12E9 detergent and hydroxyapatite; (b) binds purine and pyrimidine nucleoside triphosphates with low micromolar affinity; (c) binds GDP with the same affinity as GDP inhibits superoxide-stimulated uncoupling by kidney mitochondria; and (d) exhibits a different nucleotide preference than kidney mitochondria. Guanosine Diphosphate 281-284 uncoupling protein 2 Homo sapiens 26-30 12030845-10 2002 These data indicate that: UCP2 (a) is at least partially refolded from sarkosyl-solubilized bacterial inclusion bodies by a two-step treatment with C12E9 detergent and hydroxyapatite; (b) binds purine and pyrimidine nucleoside triphosphates with low micromolar affinity; (c) binds GDP with the same affinity as GDP inhibits superoxide-stimulated uncoupling by kidney mitochondria; and (d) exhibits a different nucleotide preference than kidney mitochondria. Guanosine Diphosphate 311-314 uncoupling protein 2 Homo sapiens 26-30 12030845-10 2002 These data indicate that: UCP2 (a) is at least partially refolded from sarkosyl-solubilized bacterial inclusion bodies by a two-step treatment with C12E9 detergent and hydroxyapatite; (b) binds purine and pyrimidine nucleoside triphosphates with low micromolar affinity; (c) binds GDP with the same affinity as GDP inhibits superoxide-stimulated uncoupling by kidney mitochondria; and (d) exhibits a different nucleotide preference than kidney mitochondria. Superoxides 324-334 uncoupling protein 2 Homo sapiens 26-30 12223452-6 2002 In contrast, nuclear vitamin D receptor (nVDR) knockout via antisense oligodeoxynucleotide (ODN) prevented the inhibitory effect of 1alpha,25-(OH)2-D3 on adipocyte UCP2 expression and protein levels. Oligodeoxyribonucleotides 92-95 uncoupling protein 2 Homo sapiens 164-168 12223452-6 2002 In contrast, nuclear vitamin D receptor (nVDR) knockout via antisense oligodeoxynucleotide (ODN) prevented the inhibitory effect of 1alpha,25-(OH)2-D3 on adipocyte UCP2 expression and protein levels. 25-(oh)2-d3 139-150 uncoupling protein 2 Homo sapiens 164-168 12223452-7 2002 These data indicate that 1a,25-(OH)2-D3 exerts an inhibitory effect on adipocyte UCP2 expression via the nVDR. 1a,25-(oh)2-d3 25-39 uncoupling protein 2 Homo sapiens 81-85 12465744-3 2002 In pancreatic islet beta cells, induction of UCP2 is shown to inhibit glucose-stimulated insulin secretion. Glucose 70-77 uncoupling protein 2 Homo sapiens 45-49 12011039-3 2002 We demonstrate in HeLa cells but not in normal diploid fibroblasts that modest increases in the expression level of uncoupling protein 2 (UCP-2) leads to a rapid and dramatic fall in mitochondrial membrane potential and to a reduction of mitochondrial NADH and intracellular ATP. NAD 252-256 uncoupling protein 2 Homo sapiens 116-136 12011039-3 2002 We demonstrate in HeLa cells but not in normal diploid fibroblasts that modest increases in the expression level of uncoupling protein 2 (UCP-2) leads to a rapid and dramatic fall in mitochondrial membrane potential and to a reduction of mitochondrial NADH and intracellular ATP. NAD 252-256 uncoupling protein 2 Homo sapiens 138-143 12011039-3 2002 We demonstrate in HeLa cells but not in normal diploid fibroblasts that modest increases in the expression level of uncoupling protein 2 (UCP-2) leads to a rapid and dramatic fall in mitochondrial membrane potential and to a reduction of mitochondrial NADH and intracellular ATP. Adenosine Triphosphate 275-278 uncoupling protein 2 Homo sapiens 116-136 12011039-3 2002 We demonstrate in HeLa cells but not in normal diploid fibroblasts that modest increases in the expression level of uncoupling protein 2 (UCP-2) leads to a rapid and dramatic fall in mitochondrial membrane potential and to a reduction of mitochondrial NADH and intracellular ATP. Adenosine Triphosphate 275-278 uncoupling protein 2 Homo sapiens 138-143 12107378-6 2002 Concerning the hypermetabolism in cachexia, new evidence supports previous theories that uncoupling protein-2 and 3 are primarily involved in the generation of reactive oxygen species and in the control of fatty acid flux across the mitochondrial membrane, respectively. Reactive Oxygen Species 160-183 uncoupling protein 2 Homo sapiens 89-115 12107378-6 2002 Concerning the hypermetabolism in cachexia, new evidence supports previous theories that uncoupling protein-2 and 3 are primarily involved in the generation of reactive oxygen species and in the control of fatty acid flux across the mitochondrial membrane, respectively. Fatty Acids 206-216 uncoupling protein 2 Homo sapiens 89-115 11904148-0 2002 Glucose induces and leptin decreases expression of uncoupling protein-2 mRNA in human islets. Glucose 0-7 uncoupling protein 2 Homo sapiens 51-71 11904148-6 2002 Glucose dose-dependently increased UCP-2 expression in all islet batches, maximally by three-fold. Glucose 0-7 uncoupling protein 2 Homo sapiens 35-40 12418549-1 2002 Reconstitution of novel mitochondrial uncoupling proteins, human UCP2 and UCP3, expressed in yeast, was performed to characterize fatty acid (FA)-induced H+ efflux in the resulted proteoliposomes. Fatty Acids 130-140 uncoupling protein 2 Homo sapiens 65-69 11935148-8 2002 Inhibition by uncoupling protein-2 of reactive oxygen species formation in macrophages and other tissues could have implications for regulation of immune function. Reactive Oxygen Species 38-61 uncoupling protein 2 Homo sapiens 14-34 11929192-7 2002 While UCP1 has a clear role in energy homeostasis, the newcomers UCP2-UCP5 may have more delicate physiological importance acting as free radical oxygen scavengers and in the regulation of ATP-dependent processes, such as secretion. Oxygen 146-152 uncoupling protein 2 Homo sapiens 65-69 11929192-7 2002 While UCP1 has a clear role in energy homeostasis, the newcomers UCP2-UCP5 may have more delicate physiological importance acting as free radical oxygen scavengers and in the regulation of ATP-dependent processes, such as secretion. Adenosine Triphosphate 189-192 uncoupling protein 2 Homo sapiens 65-69 12418549-2 2002 We now demonstrate for the first time that representatives of physiologically abundant long chain FAs, saturated or unsaturated, activate H+ translocation in UCP2- and UCP3-proteoliposomes. ammonium ferrous sulfate 98-101 uncoupling protein 2 Homo sapiens 158-162 12418549-4 2002 We have confirmed that ATP and GTP inhibit such FA-induced H+ uniport mediated by UCP2 and UCP3. Adenosine Triphosphate 23-26 uncoupling protein 2 Homo sapiens 82-86 12418549-4 2002 We have confirmed that ATP and GTP inhibit such FA-induced H+ uniport mediated by UCP2 and UCP3. Guanosine Triphosphate 31-34 uncoupling protein 2 Homo sapiens 82-86 11470240-2 2001 Treatment of C2C12 myotubes for 24 h with 40 microM etomoxir, an irreversible inhibitor of carnitine palmitoyltransferase I (CPT-I), up-regulated uncoupling protein 3 (UCP-3) mRNA levels (2-fold induction), whereas UCP-2 mRNA levels were not modified. etomoxir 52-60 uncoupling protein 2 Homo sapiens 215-220 11468281-5 2001 They are analogues of fatty acids, and they are transported by UCP1, UCP2, and UCP3. Fatty Acids 22-33 uncoupling protein 2 Homo sapiens 69-73 11701434-0 2001 Polyunsaturated fatty acids stimulate hepatic UCP-2 expression via a PPARalpha-mediated pathway. Fatty Acids, Unsaturated 0-27 uncoupling protein 2 Homo sapiens 46-51 11701434-2 2001 Thus we hypothesized that UCP-2 would be regulated in the hepatocyte by fatty acids, which are known to control other energy-related metabolic processes. Fatty Acids 72-83 uncoupling protein 2 Homo sapiens 26-31 11701434-4 2001 Eicosapentaenoic acid (EPA), a polyunsaturated fatty acid, exerted a 50-fold upregulation of UCP-2 that was concentration dependent. Eicosapentaenoic Acid 0-21 uncoupling protein 2 Homo sapiens 93-98 11701434-4 2001 Eicosapentaenoic acid (EPA), a polyunsaturated fatty acid, exerted a 50-fold upregulation of UCP-2 that was concentration dependent. Eicosapentaenoic Acid 23-26 uncoupling protein 2 Homo sapiens 93-98 11701434-4 2001 Eicosapentaenoic acid (EPA), a polyunsaturated fatty acid, exerted a 50-fold upregulation of UCP-2 that was concentration dependent. Fatty Acids, Unsaturated 31-57 uncoupling protein 2 Homo sapiens 93-98 11701434-5 2001 This effect was seen within 12 h and was maximal by 36 h. Aspirin blocked the induction of UCP-2 by EPA, indicating involvement of the prostaglandin pathway. Aspirin 58-65 uncoupling protein 2 Homo sapiens 91-96 11701434-5 2001 This effect was seen within 12 h and was maximal by 36 h. Aspirin blocked the induction of UCP-2 by EPA, indicating involvement of the prostaglandin pathway. Eicosapentaenoic Acid 100-103 uncoupling protein 2 Homo sapiens 91-96 11701434-5 2001 This effect was seen within 12 h and was maximal by 36 h. Aspirin blocked the induction of UCP-2 by EPA, indicating involvement of the prostaglandin pathway. Prostaglandins 135-148 uncoupling protein 2 Homo sapiens 91-96 11701434-6 2001 Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. Arachidonic Acid 25-41 uncoupling protein 2 Homo sapiens 140-145 11701434-6 2001 Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. Arachidonic Acid 25-41 uncoupling protein 2 Homo sapiens 229-234 11701434-6 2001 Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. Prostaglandins 74-88 uncoupling protein 2 Homo sapiens 140-145 11701434-6 2001 Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. Prostaglandins 74-88 uncoupling protein 2 Homo sapiens 229-234 11701434-6 2001 Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. Aspirin 108-115 uncoupling protein 2 Homo sapiens 140-145 11701434-6 2001 Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. Aspirin 108-115 uncoupling protein 2 Homo sapiens 229-234 11701434-6 2001 Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. Prostaglandins 192-206 uncoupling protein 2 Homo sapiens 140-145 11701434-6 2001 Hepatocytes treated with arachidonic acid, the immediate precursor to the prostaglandins, also exhibited an aspirin-inhibitable increase in UCP-2 levels, further supporting the involvement of prostaglandins in regulating hepatic UCP-2. Prostaglandins 192-206 uncoupling protein 2 Homo sapiens 229-234 11701434-7 2001 The peroxisome proliferator-activated receptor-alpha (PPARalpha) agonist Wy-14643 stimulated UCP-2 mRNA levels as effectively as EPA. pirinixic acid 73-81 uncoupling protein 2 Homo sapiens 93-98 11701434-8 2001 These data indicate that UCP-2 is upregulated by polyunsaturated fatty acids, potentially through a prostaglandin/PPARalpha-mediated pathway. Fatty Acids, Unsaturated 49-76 uncoupling protein 2 Homo sapiens 25-30 11701434-8 2001 These data indicate that UCP-2 is upregulated by polyunsaturated fatty acids, potentially through a prostaglandin/PPARalpha-mediated pathway. Prostaglandins 100-113 uncoupling protein 2 Homo sapiens 25-30 11709071-6 2001 We conclude that CoQ acted in mitochondria through production of superoxide, which mediated uncoupling, probably by acting through uncoupling protein 2. coenzyme Q10 17-20 uncoupling protein 2 Homo sapiens 131-151 11709071-6 2001 We conclude that CoQ acted in mitochondria through production of superoxide, which mediated uncoupling, probably by acting through uncoupling protein 2. Superoxides 65-75 uncoupling protein 2 Homo sapiens 131-151 11641751-13 2001 Also mean plasma TSH concentrations 20, 30 and 45 min after the TRH injection increased more with overfeeding among UCP2 A55V (P<0.005) and UCP3 Rsa I CC (P=0.017) subjects. Thyrotropin 17-20 uncoupling protein 2 Homo sapiens 116-120 11641751-15 2001 The association of the UCP2 A55V and UCP3 Rsa I CC genotypes with a greater increase in the TSH response to TRH load could reflect a compensatory mechanism counteracting the effects of overfeeding. Thyrotropin 92-95 uncoupling protein 2 Homo sapiens 23-27 11311236-6 2001 These results suggested that catecholamines up-regulate UCP2 and UCP3 expression through direct action on the beta2-AR in skeletal muscle. Catecholamines 29-43 uncoupling protein 2 Homo sapiens 56-60 11381268-3 2001 Like its close relatives UCP1 and UCP3, UCP2 uncouples proton entry in the mitochondrial matrix from ATP synthesis and is therefore a candidate gene for obesity. Adenosine Triphosphate 101-104 uncoupling protein 2 Homo sapiens 40-44 11311236-4 2001 Stimulation of the cells with epinephrine increased the UCP3 mRNA level transiently at 6 h, and also the UCP2 mRNA level at 6-24 h. The stimulatory effects of epinephrine were also observed in the presence of carbacyclin and 9-cis retinoic acid, and mimicked by isoproterenol and salbutamol (beta2-AR agonists), but abolished by propranolol and ICI-118,551 (beta2-AR antagonists). Epinephrine 30-41 uncoupling protein 2 Homo sapiens 105-109 11311236-4 2001 Stimulation of the cells with epinephrine increased the UCP3 mRNA level transiently at 6 h, and also the UCP2 mRNA level at 6-24 h. The stimulatory effects of epinephrine were also observed in the presence of carbacyclin and 9-cis retinoic acid, and mimicked by isoproterenol and salbutamol (beta2-AR agonists), but abolished by propranolol and ICI-118,551 (beta2-AR antagonists). Epinephrine 159-170 uncoupling protein 2 Homo sapiens 105-109 11278377-0 2001 The regulation of uncoupling protein-2 gene expression by omega-6 polyunsaturated fatty acids in human skeletal muscle cells involves multiple pathways, including the nuclear receptor peroxisome proliferator-activated receptor beta. omega-6 polyunsaturated fatty acids 58-93 uncoupling protein 2 Homo sapiens 18-38 11319645-2 2001 In a group of endurance athletes, with a range in fiber type distribution, we hypothesized that the effect of the high-fat diet on UCP2 and UCP3 mRNA expression is more pronounced in muscle fibers which are known to have a high capacity to shift from carbohydrate to fat oxidation (type IIA fibers). Carbohydrates 251-263 uncoupling protein 2 Homo sapiens 131-135 11319648-0 2001 The association between the val/ala-55 polymorphism of the uncoupling protein 2 gene and exercise efficiency. Valine 28-31 uncoupling protein 2 Homo sapiens 59-79 11319648-0 2001 The association between the val/ala-55 polymorphism of the uncoupling protein 2 gene and exercise efficiency. Alanine 32-35 uncoupling protein 2 Homo sapiens 59-79 11319648-2 2001 We have previously found an increased physical activity but a similar 24-h energy expenditure (EE) in subjects with the val/val-55 UCP2 genotype compared to those with the ala/ala genotype which indicates that the val-55 allele is statistically associated with a higher metabolic efficiency. Valine 120-123 uncoupling protein 2 Homo sapiens 131-135 11319648-2 2001 We have previously found an increased physical activity but a similar 24-h energy expenditure (EE) in subjects with the val/val-55 UCP2 genotype compared to those with the ala/ala genotype which indicates that the val-55 allele is statistically associated with a higher metabolic efficiency. Valine 124-127 uncoupling protein 2 Homo sapiens 131-135 11319648-2 2001 We have previously found an increased physical activity but a similar 24-h energy expenditure (EE) in subjects with the val/val-55 UCP2 genotype compared to those with the ala/ala genotype which indicates that the val-55 allele is statistically associated with a higher metabolic efficiency. Valine 124-127 uncoupling protein 2 Homo sapiens 131-135 11319648-8 2001 CONCLUSION: As the val/ala-55 polymorphism is located in a domain of the protein without any known function, the different exercise efficiency between the two genotypes most likely reflects a linkage disequilibrium with a functionally significant polymorphism in UCP2 or in the neighbouring UCP3 gene. Valine 19-22 uncoupling protein 2 Homo sapiens 263-267 11319648-8 2001 CONCLUSION: As the val/ala-55 polymorphism is located in a domain of the protein without any known function, the different exercise efficiency between the two genotypes most likely reflects a linkage disequilibrium with a functionally significant polymorphism in UCP2 or in the neighbouring UCP3 gene. Alanine 23-26 uncoupling protein 2 Homo sapiens 263-267 11264003-3 2001 Expression of the UCP-2 gene in clonal beta-cells (INS-1) was decreased by 45% after 48 h of culture with vitamin E and selenite. Vitamin E 106-115 uncoupling protein 2 Homo sapiens 18-23 11264003-3 2001 Expression of the UCP-2 gene in clonal beta-cells (INS-1) was decreased by 45% after 48 h of culture with vitamin E and selenite. Selenious Acid 120-128 uncoupling protein 2 Homo sapiens 18-23 11264003-6 2001 We next tested whether overexpression of UCP-2 could enhance resistance of beta-cells toward H(2)O(2) toxicity. Hydrogen Peroxide 93-101 uncoupling protein 2 Homo sapiens 41-46 11171965-0 2001 Uncoupling proteins 2 and 3 are highly active H(+) transporters and highly nucleotide sensitive when activated by coenzyme Q (ubiquinone). Ubiquinone 126-136 uncoupling protein 2 Homo sapiens 0-27 11171965-1 2001 Based on the discovery of coenzyme Q (CoQ) as an obligatory cofactor for H(+) transport by uncoupling protein 1 (UCP1) [Echtay, K. S., Winkler, E. & Klingenberg, M. (2000) Nature (London) 408, 609-613] we show here that UCP2 and UCP3 are also highly active H(+) transporters and require CoQ and fatty acid for H(+) transport, which is inhibited by low concentrations of nucleotides. Ubiquinone 38-41 uncoupling protein 2 Homo sapiens 224-228 11171965-3 2001 Human UCP2 and 3 expressed in Escherichia coli inclusion bodies are solubilized, and by exchange of sarcosyl against digitonin, nucleotide binding as measured with 2"-O-[5-(dimethylamino)naphthalene-1-sulfonyl]-GTP can be restored. sarkosyl 100-108 uncoupling protein 2 Homo sapiens 6-16 11171965-3 2001 Human UCP2 and 3 expressed in Escherichia coli inclusion bodies are solubilized, and by exchange of sarcosyl against digitonin, nucleotide binding as measured with 2"-O-[5-(dimethylamino)naphthalene-1-sulfonyl]-GTP can be restored. Digitonin 117-126 uncoupling protein 2 Homo sapiens 6-16 11171965-3 2001 Human UCP2 and 3 expressed in Escherichia coli inclusion bodies are solubilized, and by exchange of sarcosyl against digitonin, nucleotide binding as measured with 2"-O-[5-(dimethylamino)naphthalene-1-sulfonyl]-GTP can be restored. 2"-o-[5-(dimethylamino)naphthalene-1-sulfonyl]-gtp 164-214 uncoupling protein 2 Homo sapiens 6-16 11171965-9 2001 In UCP2 as in UCP1, ATP is a stronger inhibitor than ADP, but in UCP3 ADP inhibits more strongly than ATP. Adenosine Triphosphate 20-23 uncoupling protein 2 Homo sapiens 3-7 11171965-11 2001 These results confirm the regulation of UCP2 and UCP3 by the same factors CoQ, fatty acids, and nucleotides as UCP1. Ubiquinone 74-77 uncoupling protein 2 Homo sapiens 40-44 11171965-11 2001 These results confirm the regulation of UCP2 and UCP3 by the same factors CoQ, fatty acids, and nucleotides as UCP1. Fatty Acids 79-90 uncoupling protein 2 Homo sapiens 40-44 11099489-0 2001 Triiodothyronine-mediated up-regulation of UCP2 and UCP3 mRNA expression in human skeletal muscle without coordinated induction of mitochondrial respiratory chain genes. Triiodothyronine 0-16 uncoupling protein 2 Homo sapiens 43-47 11502224-8 2001 The general conclusion is that UCP2 and UCP3 may have distinct primary functions, with UCP3 implicated in regulating the flux of lipid substrates across the mitochondria and UCP2 in the control of mitochondrial generation of reactive oxygen species. Reactive Oxygen Species 225-248 uncoupling protein 2 Homo sapiens 31-35 11502224-8 2001 The general conclusion is that UCP2 and UCP3 may have distinct primary functions, with UCP3 implicated in regulating the flux of lipid substrates across the mitochondria and UCP2 in the control of mitochondrial generation of reactive oxygen species. Reactive Oxygen Species 225-248 uncoupling protein 2 Homo sapiens 174-178 11278377-2 2001 We have identified, at least in part, the mechanism by which polyunsaturated fatty acids increase UCP-2 expression in primary culture of human muscle cells. Fatty Acids, Unsaturated 61-88 uncoupling protein 2 Homo sapiens 98-103 11278377-3 2001 omega-6 fatty acids and arachidonic acid induced a 3-fold rise in UCP-2 mRNA levels possibly through transcriptional activation. Fatty Acids, Omega-6 0-19 uncoupling protein 2 Homo sapiens 66-71 11278377-3 2001 omega-6 fatty acids and arachidonic acid induced a 3-fold rise in UCP-2 mRNA levels possibly through transcriptional activation. Arachidonic Acid 24-40 uncoupling protein 2 Homo sapiens 66-71 11278377-6 2001 Consistent with a role of cAMP and protein kinase A, both prostaglandins induced a marked accumulation of cAMP in human myotubes, and forskolin reproduced the effect of arachidonic acid on UCP-2 mRNA expression. Colforsin 134-143 uncoupling protein 2 Homo sapiens 189-194 11278377-6 2001 Consistent with a role of cAMP and protein kinase A, both prostaglandins induced a marked accumulation of cAMP in human myotubes, and forskolin reproduced the effect of arachidonic acid on UCP-2 mRNA expression. Arachidonic Acid 169-185 uncoupling protein 2 Homo sapiens 189-194 11278377-7 2001 Inhibition of protein kinase A with H-89 suppressed the effect of PGE(2), whereas cPGI(2) and arachidonic acid were still able to increase ucp-2 gene expression, suggesting additional mechanisms. cpgi(2) 82-89 uncoupling protein 2 Homo sapiens 139-144 11278377-7 2001 Inhibition of protein kinase A with H-89 suppressed the effect of PGE(2), whereas cPGI(2) and arachidonic acid were still able to increase ucp-2 gene expression, suggesting additional mechanisms. Arachidonic Acid 94-110 uncoupling protein 2 Homo sapiens 139-144 11278377-11 2001 These results suggest thus that ucp-2 gene expression is regulated by omega-6 fatty acids in human muscle cells through mechanisms involving at least protein kinase A and the nuclear receptor PPARbeta. Fatty Acids, Omega-6 70-89 uncoupling protein 2 Homo sapiens 32-37 11098051-1 2001 Uncoupling protein 2 (UCP2) belongs to the mitochondrial anion carrier family and partially uncouples respiration from ATP synthesis when expressed in recombinant yeast mitochondria. Adenosine Triphosphate 119-122 uncoupling protein 2 Homo sapiens 0-20 11239486-10 2001 The regulation of UCP1 and UCP2 by retinoids and the lack of effects of fatty acids on UCP2 or UCP3 are starting to set differences among the new uncoupling proteins. Retinoids 35-44 uncoupling protein 2 Homo sapiens 27-31 11179956-0 2001 Structure-function relationships in UCP1, UCP2 and chimeras: EPR analysis and retinoic acid activation of UCP2. Tretinoin 78-91 uncoupling protein 2 Homo sapiens 106-110 11179956-5 2001 Our results confirm the observations reported for recombinant yeast that retinoic acid, but not fatty acids known to activate UCP1, activates proton transport by UCP2 and that this activation is insensitive to nucleotide inhibition. Tretinoin 73-86 uncoupling protein 2 Homo sapiens 162-166 11179956-9 2001 In the other chimeric construct U2U1, hUCP2 (amino acids 1-210) and mUCP1 (amino acids 199-307), retinoic acid still acted as an activator, but no inhibition was observed with GTP. Tretinoin 97-110 uncoupling protein 2 Homo sapiens 38-43 11179956-11 2001 The EPR data show large structural changes in UCP1 and UCP2 on exposure to ATP, implying that a putative nucleotide-binding site is present on UCP2. Adenosine Triphosphate 75-78 uncoupling protein 2 Homo sapiens 55-59 11179956-11 2001 The EPR data show large structural changes in UCP1 and UCP2 on exposure to ATP, implying that a putative nucleotide-binding site is present on UCP2. Adenosine Triphosphate 75-78 uncoupling protein 2 Homo sapiens 143-147 11179956-12 2001 EPR analysis also demonstrated changes in conformation of UCP1/UCP2 chimeras following exposure to purine nucleotides. Purine Nucleotides 99-117 uncoupling protein 2 Homo sapiens 63-67 11061990-0 2000 Dietary fatty acids Up-regulate the expression of UCP2 in 3T3-L1 preadipocytes. dietary fatty acids 0-19 uncoupling protein 2 Homo sapiens 50-54 11061990-1 2000 States characterised by elevated plasma fatty acid levels are accompanied by increased UCP2 expression but the physiological regulation of UCP2 expression in white adipose tissue is not fully understood. Fatty Acids 40-50 uncoupling protein 2 Homo sapiens 87-91 11061990-2 2000 We used 3T3-L1 preadipocytes to determine whether various dietary fatty acids (20:5, 18:3, 18:2, 18:1, 18:0) directly regulate UCP2 expression. Fatty Acids 66-77 uncoupling protein 2 Homo sapiens 127-131 11061990-3 2000 Physiological concentrations of each class of polyunsaturated fatty acid and the monounsaturated fatty acid dramatically up-regulated UCP2 mRNA levels 5- to 8-fold, but the saturated fatty acid was not so effective (1.5-fold). Fatty Acids, Unsaturated 46-72 uncoupling protein 2 Homo sapiens 134-138 11061990-3 2000 Physiological concentrations of each class of polyunsaturated fatty acid and the monounsaturated fatty acid dramatically up-regulated UCP2 mRNA levels 5- to 8-fold, but the saturated fatty acid was not so effective (1.5-fold). Fatty Acids, Monounsaturated 81-107 uncoupling protein 2 Homo sapiens 134-138 11061990-3 2000 Physiological concentrations of each class of polyunsaturated fatty acid and the monounsaturated fatty acid dramatically up-regulated UCP2 mRNA levels 5- to 8-fold, but the saturated fatty acid was not so effective (1.5-fold). Fatty Acids 52-72 uncoupling protein 2 Homo sapiens 134-138 11061990-6 2000 In conclusion, dietary unsaturated fatty acids may be physiological signals to alter energy balance by direct induction of UCP2. Fatty Acids, Unsaturated 23-46 uncoupling protein 2 Homo sapiens 123-127 10843184-3 2000 We also investigated whether the thiazolidinedione, troglitazone, stimulates UCP2 and UCP3 mRNA levels to follow up on the observation that this antidiabetic drug increases the levels of expression in cultured cells. 2,4-thiazolidinedione 33-50 uncoupling protein 2 Homo sapiens 77-81 10852469-3 2000 UCP-2 messenger ribonucleic acid expression was quantified by nuclease protection in adipose tissue from lean and obese humans in both the fasting and postprandial states. ribonucleic 16-27 uncoupling protein 2 Homo sapiens 0-5 10852469-5 2000 In the fasting state UCP-2 expression correlated inversely with body mass index (r = -0.45; P = 0.026), percent body fat (r = -0.41; P = 0.05), plasma insulin (r = -0.47; P = 0.02), epigastric venous fatty acids (r = -0.45; P = 0.04), and leptin (r = -0.50; P = 0.018). Fatty Acids 200-211 uncoupling protein 2 Homo sapiens 21-26 10852469-8 2000 In conclusion, 1) UCP-2 messenger ribonucleic acid expression in sc adipose tissue is inversely related to adiposity and independently linked to local plasma leptin levels; and 2) UCP-2 expression is not acutely regulated by food intake, insulin, or fatty acids. Fatty Acids 250-261 uncoupling protein 2 Homo sapiens 18-23 10849580-0 2000 Depot-related and thiazolidinedione-responsive expression of uncoupling protein 2 (UCP2) in human adipocytes. 2,4-thiazolidinedione 18-35 uncoupling protein 2 Homo sapiens 61-81 10849580-0 2000 Depot-related and thiazolidinedione-responsive expression of uncoupling protein 2 (UCP2) in human adipocytes. 2,4-thiazolidinedione 18-35 uncoupling protein 2 Homo sapiens 83-87 10849580-2 2000 Studies were undertaken (1) to establish whether the expression of UCP2 mRNA varies in a depot-related manner in isolated human adipocytes, (2) to determine whether thiazolidinedione exposure influences the expression of UCP2 mRNA in cultured human pre-adipocytes, and (3) to determine whether human UCP2 is targeted to mitochondria when transfected into mammalian cells. 2,4-thiazolidinedione 165-182 uncoupling protein 2 Homo sapiens 221-225 10849580-2 2000 Studies were undertaken (1) to establish whether the expression of UCP2 mRNA varies in a depot-related manner in isolated human adipocytes, (2) to determine whether thiazolidinedione exposure influences the expression of UCP2 mRNA in cultured human pre-adipocytes, and (3) to determine whether human UCP2 is targeted to mitochondria when transfected into mammalian cells. 2,4-thiazolidinedione 165-182 uncoupling protein 2 Homo sapiens 221-225 10849580-4 2000 MEASUREMENTS: A competitive reverse transcriptase-polymerase chain reaction (RT-PCR) was used to quantify UCP2 mRNA expression in human omental and subcutaneous adipocytes, and in cultured human preadipocytes differentiated in vitro using the thiazolidinedione, BRL49653. 2,4-thiazolidinedione 243-260 uncoupling protein 2 Homo sapiens 106-110 11003590-0 2000 Nitric oxide-dependent downregulation of adipocyte UCP-2 expression by tumor necrosis factor-alpha. Nitric Oxide 0-12 uncoupling protein 2 Homo sapiens 51-56 11003590-6 2000 Cell treatment with the NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME; 1 mmol/l) significantly diminished the TNF-alpha-mediated sustained downregulation of UCP-2 expression, whereas cell treatment with a nitric oxide (NO) donor (10(-3) mol/l S-nitroso-L-glutathione) mimicked the TNF-alpha effect on UCP-2 expression. NG-Nitroarginine Methyl Ester 38-72 uncoupling protein 2 Homo sapiens 168-173 11003590-6 2000 Cell treatment with the NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME; 1 mmol/l) significantly diminished the TNF-alpha-mediated sustained downregulation of UCP-2 expression, whereas cell treatment with a nitric oxide (NO) donor (10(-3) mol/l S-nitroso-L-glutathione) mimicked the TNF-alpha effect on UCP-2 expression. NG-Nitroarginine Methyl Ester 38-72 uncoupling protein 2 Homo sapiens 312-317 11003590-6 2000 Cell treatment with the NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME; 1 mmol/l) significantly diminished the TNF-alpha-mediated sustained downregulation of UCP-2 expression, whereas cell treatment with a nitric oxide (NO) donor (10(-3) mol/l S-nitroso-L-glutathione) mimicked the TNF-alpha effect on UCP-2 expression. NG-Nitroarginine Methyl Ester 74-80 uncoupling protein 2 Homo sapiens 168-173 11003590-6 2000 Cell treatment with the NOS inhibitor N(G)-nitro-L-arginine methyl ester (L-NAME; 1 mmol/l) significantly diminished the TNF-alpha-mediated sustained downregulation of UCP-2 expression, whereas cell treatment with a nitric oxide (NO) donor (10(-3) mol/l S-nitroso-L-glutathione) mimicked the TNF-alpha effect on UCP-2 expression. NG-Nitroarginine Methyl Ester 74-80 uncoupling protein 2 Homo sapiens 312-317 10843184-3 2000 We also investigated whether the thiazolidinedione, troglitazone, stimulates UCP2 and UCP3 mRNA levels to follow up on the observation that this antidiabetic drug increases the levels of expression in cultured cells. Troglitazone 52-64 uncoupling protein 2 Homo sapiens 77-81 10843184-5 2000 Only nocturnal urinary norepinephrine excretion could explain a significant fraction of the variability in both UCP2 and UCP3 expression in muscle, but not adipose tissue. Norepinephrine 23-37 uncoupling protein 2 Homo sapiens 112-116 10557023-3 1999 We studied the role of a common amino acid substitution, replacing an alanine (A) with a valine (V) at codon 55, of the coding region of the UCP2 gene for 24-h energy expenditure and respiratory quotient (RQ) in healthy subjects METHODS: 24-h energy expenditure and RQ were measured in calorimeters in 60 healthy subjects. Alanine 70-77 uncoupling protein 2 Homo sapiens 141-145 10575039-4 1999 UCP2-producing neurons were found to be the targets of peripheral hormones, including leptin and gonadal steroids, and the presence of UCP2 protein in axonal processes predicted increased local brain mitochondrial uncoupling activity and heat production. Steroids 105-113 uncoupling protein 2 Homo sapiens 0-4 10548525-2 1999 Rosiglitazone (10(-6) M, 4 h) increased p85alphaphosphatidylinositol 3-kinase (p85alphaPI-3K) and uncoupling protein-2 mRNA levels and decreased leptin expression. Rosiglitazone 0-13 uncoupling protein 2 Homo sapiens 79-118 10566652-8 1999 Furthermore, plasma free fatty acids were positively correlated with the expression of UCP2 (r = 0.573; P < 0.01) and UCP3 (r = 0.518; P < 0.05) in skeletal muscle. Fatty Acids, Nonesterified 20-36 uncoupling protein 2 Homo sapiens 87-91 10751530-3 2000 METHODS: The genotypes of A55V variant in the UCP2 gene were determined by a PCR-RFLP assay in 359 unrelated Chinese [including 193 normal glucose tolerance(NGT) and 166 type 2 diabetic subjects by ADA 97" criteria]. Glucose 139-146 uncoupling protein 2 Homo sapiens 46-50 10751530-6 2000 A55V variant in the UCP2 gene was also associated with serum triglyceride (TG) level (P=0.0072) in males. Triglycerides 61-73 uncoupling protein 2 Homo sapiens 20-24 10751530-6 2000 A55V variant in the UCP2 gene was also associated with serum triglyceride (TG) level (P=0.0072) in males. Triglycerides 75-77 uncoupling protein 2 Homo sapiens 20-24 10557023-3 1999 We studied the role of a common amino acid substitution, replacing an alanine (A) with a valine (V) at codon 55, of the coding region of the UCP2 gene for 24-h energy expenditure and respiratory quotient (RQ) in healthy subjects METHODS: 24-h energy expenditure and RQ were measured in calorimeters in 60 healthy subjects. Valine 89-95 uncoupling protein 2 Homo sapiens 141-145 10653474-7 1999 The fatty acid cycling mechanism is predicted, as well for the recently discovered uncoupling proteins, UCP2 and UCP3. Fatty Acids 4-14 uncoupling protein 2 Homo sapiens 104-108 10071761-15 1999 Mutational analysis of the hUCP2 gene in a cohort of 25 children of caucasian origin (aged 7-13) characterized by low BMR values revealed a point mutation in exon 4 (C to T transition at position 164 of the corresponding cDNA resulting in the substitution of an alanine residue by a valine at codon 55) and an insertion polymorphism in exon 8. Alanine 262-269 uncoupling protein 2 Homo sapiens 27-32 10412376-2 1999 One hour after administration of the beta 3-adrenoceptor agonist Trecadrine, a statistically significant increase in UCP1 messenger RNA (mRNA) expression in BAT was observed, whereas UCP2 and UCP3 in both BAT and gastrocnemius muscle were unaffected. trecadrine 65-75 uncoupling protein 2 Homo sapiens 183-187 10454122-6 1999 In contrast, association studies of UCP2 using an Ala to Val variant at amino acid 55 have produced negative results. Alanine 50-53 uncoupling protein 2 Homo sapiens 36-40 10454122-6 1999 In contrast, association studies of UCP2 using an Ala to Val variant at amino acid 55 have produced negative results. Valine 57-60 uncoupling protein 2 Homo sapiens 36-40 10454128-1 1999 Uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3) are mitochondrial proteins that may play a role in the control of energy expenditure by uncoupling respiration from ATP synthesis. Adenosine Triphosphate 176-179 uncoupling protein 2 Homo sapiens 0-20 10454128-1 1999 Uncoupling protein-2 (UCP2) and uncoupling protein-3 (UCP3) are mitochondrial proteins that may play a role in the control of energy expenditure by uncoupling respiration from ATP synthesis. Adenosine Triphosphate 176-179 uncoupling protein 2 Homo sapiens 22-26 10333043-8 1999 Short-term incubation for 4 and 24 h with thiazolidinediones increased uncoupling protein 2 expression 1.35-fold and 2.3-fold, respectively. Thiazolidinediones 42-60 uncoupling protein 2 Homo sapiens 71-91 10333043-10 1999 CONCLUSION/INTERPRETATION: Thiazolidinediones rapidly increase the expression of uncoupling protein 2 in human PAZ6 adipocytes but the increase of uncoupling protein 2 expression is always associated with an augmentation of the expression of all adipocyte markers studied in parallel. Thiazolidinediones 27-45 uncoupling protein 2 Homo sapiens 81-101 10333043-11 1999 This indicates that the effect of thiazolidinediones on uncoupling protein 2 mRNA reflects a general increase in adipocyte differentiation rather than a specific augmentation of uncoupling protein 2 gene expression. Thiazolidinediones 34-52 uncoupling protein 2 Homo sapiens 56-76 10382588-4 1999 We investigated a recently identified variant of the UCP2 gene in exon 8 as a marker for glucose and weight homeostasis. Glucose 89-96 uncoupling protein 2 Homo sapiens 53-57 10382588-5 1999 METHODS: Length variation of the UCP2 exon 8 variant was studied by the polymerase chain reaction and agarose gel electrophoresis. Sepharose 102-109 uncoupling protein 2 Homo sapiens 33-37 10333043-0 1999 Effect of thiazolidinediones on expression of UCP2 and adipocyte markers in human PAZ6 adipocytes. Thiazolidinediones 10-28 uncoupling protein 2 Homo sapiens 46-50 10333043-1 1999 AIMS/HYPOTHESIS: Thiazolidinediones, a new class of insulin sensitizers, up-regulate the expression of uncoupling protein 2 in rodent adipocytes. Thiazolidinediones 17-35 uncoupling protein 2 Homo sapiens 103-123 10333043-2 1999 It is not known, however, whether thiazolidinediones influence uncoupling protein 2 expression in human adipocytes. Thiazolidinediones 34-52 uncoupling protein 2 Homo sapiens 63-83 10333043-6 1999 RESULTS: When cells were differentiated 15 days in the presence of thiazolidinediones, uncoupling protein 2 expression was 2.1-fold higher than in the absence of the drugs. Thiazolidinediones 67-85 uncoupling protein 2 Homo sapiens 87-107 10066437-3 1999 Analysis of changes in metabolic parameters suggested that fatty acids may be associated with the increased UCP2 mRNA level. Fatty Acids 59-70 uncoupling protein 2 Homo sapiens 108-112 10066437-9 1999 The data support the hypothesis that fatty acids are involved in the control of adipocyte UCP2 mRNA expression in humans. Fatty Acids 37-48 uncoupling protein 2 Homo sapiens 90-94 10071761-15 1999 Mutational analysis of the hUCP2 gene in a cohort of 25 children of caucasian origin (aged 7-13) characterized by low BMR values revealed a point mutation in exon 4 (C to T transition at position 164 of the corresponding cDNA resulting in the substitution of an alanine residue by a valine at codon 55) and an insertion polymorphism in exon 8. Valine 283-289 uncoupling protein 2 Homo sapiens 27-32 10023736-7 1999 Surprisingly, fasting has a stimulatory effect on UCP2 and UCP3 mRNA levels, possibly explained by the effects of free fatty acid on UCP2 and UCP3 gene expression. Fatty Acids, Nonesterified 114-129 uncoupling protein 2 Homo sapiens 133-137 9891988-0 1998 9-cis retinoic acid induces the expression of the uncoupling protein-2 gene in brown adipocytes. Alitretinoin 0-19 uncoupling protein 2 Homo sapiens 50-70 9891988-3 1998 9-cis Retinoic acid causes a dose-dependent induction of UCP2 mRNA levels in brown adipocytes, whereas all-trans retinoic acid has no effect. Alitretinoin 0-19 uncoupling protein 2 Homo sapiens 57-61 9891988-5 1998 9-cis Retinoic acid, acting through RXR receptors, is identified as a major regulator of the expression of the UCP2 gene in the brown fat cell. Alitretinoin 0-19 uncoupling protein 2 Homo sapiens 111-115 9836527-3 1998 In normal glucose-tolerant individuals, muscle UCP2 mRNA levels were positively correlated with percentage of body fat and with BMI (r = 0.6 and P < 0.05 for both). Glucose 10-17 uncoupling protein 2 Homo sapiens 47-51 9271366-0 1997 A role for uncoupling protein-2 as a regulator of mitochondrial hydrogen peroxide generation. Hydrogen Peroxide 64-81 uncoupling protein 2 Homo sapiens 11-31 10023736-1 1999 Uncoupling protein (UCP) 2 and UCP3 are newly discovered proteins that can uncouple ATP production from mitochondrial respiration, thereby dissipating energy as heat and affecting energy metabolism efficiency. Adenosine Triphosphate 84-87 uncoupling protein 2 Homo sapiens 0-26 10023736-7 1999 Surprisingly, fasting has a stimulatory effect on UCP2 and UCP3 mRNA levels, possibly explained by the effects of free fatty acid on UCP2 and UCP3 gene expression. Fatty Acids, Nonesterified 114-129 uncoupling protein 2 Homo sapiens 50-54 9709950-9 1998 These results indicate that the Ala55 --> Val and Ala232 --> Thr variants of UCP2 do not play an important role in the pathogenesis of NIDDM or obesity in the Japanese population. Threonine 67-70 uncoupling protein 2 Homo sapiens 83-87 9661627-2 1998 By uncoupling respiration from ATP synthesis, UCP2 might be involved in the control of energy expenditure. Adenosine Triphosphate 31-34 uncoupling protein 2 Homo sapiens 46-50 9693744-5 1998 Fatty acid cycling was documented for UCP-1, PUMP and ADP/ATP carrier, and is predicted also for UCP-2 and UCP-3. Fatty Acids 0-10 uncoupling protein 2 Homo sapiens 97-102 9693744-8 1998 UCP-2 is probably involved in the regulation of body weight and energy balance, in fever, and defense against generation of reactive oxygen species. Reactive Oxygen Species 124-147 uncoupling protein 2 Homo sapiens 0-5 9421444-5 1998 Within 4 h of exposing these cells to 30 microM darglitazone, there was an increase in UCP2 mRNA which reached a plateau of 5-10 times the basal in about 8 h. In all cells TZDs (darglitazone, troglitazone) were more active than the predominantly PPAR alpha ligands WY-14,613 and clofibrate, or the non-selective ligand linoleic acid. darglitazone 48-60 uncoupling protein 2 Homo sapiens 87-91 9421444-5 1998 Within 4 h of exposing these cells to 30 microM darglitazone, there was an increase in UCP2 mRNA which reached a plateau of 5-10 times the basal in about 8 h. In all cells TZDs (darglitazone, troglitazone) were more active than the predominantly PPAR alpha ligands WY-14,613 and clofibrate, or the non-selective ligand linoleic acid. tryptophyltyrosine 265-267 uncoupling protein 2 Homo sapiens 87-91 9421444-5 1998 Within 4 h of exposing these cells to 30 microM darglitazone, there was an increase in UCP2 mRNA which reached a plateau of 5-10 times the basal in about 8 h. In all cells TZDs (darglitazone, troglitazone) were more active than the predominantly PPAR alpha ligands WY-14,613 and clofibrate, or the non-selective ligand linoleic acid. Clofibrate 279-289 uncoupling protein 2 Homo sapiens 87-91 9421444-5 1998 Within 4 h of exposing these cells to 30 microM darglitazone, there was an increase in UCP2 mRNA which reached a plateau of 5-10 times the basal in about 8 h. In all cells TZDs (darglitazone, troglitazone) were more active than the predominantly PPAR alpha ligands WY-14,613 and clofibrate, or the non-selective ligand linoleic acid. Linoleic Acid 319-332 uncoupling protein 2 Homo sapiens 87-91 9421444-6 1998 These results indicate that TZDs can stimulate the expression of UCP2 gene probably via PPAR gamma and hence have the potential to increase energy expenditure in adult humans, in whom UCP2 is expressed ubiquitously. tzds 28-32 uncoupling protein 2 Homo sapiens 65-69 9421444-6 1998 These results indicate that TZDs can stimulate the expression of UCP2 gene probably via PPAR gamma and hence have the potential to increase energy expenditure in adult humans, in whom UCP2 is expressed ubiquitously. tzds 28-32 uncoupling protein 2 Homo sapiens 184-188 9271366-7 1997 GDP was also able to raise membrane potential and H2O2 production of the mitochondria from nonparenchymal cells expressing UCP2, but was completely ineffective on mitochondria from hepatocytes deprived of UCP2. Guanosine Diphosphate 0-3 uncoupling protein 2 Homo sapiens 123-127 9271366-7 1997 GDP was also able to raise membrane potential and H2O2 production of the mitochondria from nonparenchymal cells expressing UCP2, but was completely ineffective on mitochondria from hepatocytes deprived of UCP2. Guanosine Diphosphate 0-3 uncoupling protein 2 Homo sapiens 205-209 9271366-8 1997 The GDP effect was also observed with mitochondrial fractions of the spleen or thymus, which highly expressed UCP2. Guanosine Diphosphate 4-7 uncoupling protein 2 Homo sapiens 110-114 9271366-9 1997 Altogether, these results strongly suggest that UCP2 is sensitive to GDP and that the UCPs, particularly UCP2, are able to modulate H2O2 mitochondrial generation. Guanosine Diphosphate 69-72 uncoupling protein 2 Homo sapiens 48-52 9271366-9 1997 Altogether, these results strongly suggest that UCP2 is sensitive to GDP and that the UCPs, particularly UCP2, are able to modulate H2O2 mitochondrial generation. Hydrogen Peroxide 132-136 uncoupling protein 2 Homo sapiens 105-109 9133562-5 1997 Furthermore, UCPH expression in yeast causes a decrease in the mitochondrial membrane potential, as judged by staining with the potential-sensitive dye DiOC6. 3,3'-dihexyl-2,2'-oxacarbocyanine 152-157 uncoupling protein 2 Homo sapiens 13-17