PMID-sentid	Pub_year	Sent_text	comp_official_name	comp_offset	protein_name	organism	prot_offset
23759102-8	2013	Ethylene levels were similar in etiolated cesa6(ret8) and det2-1 and in eto1 mutants treated with acsinone7303.	ethylene	0-8	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	58-62
29324765-2	2018	Here, a det2-9 mutant defective in BR synthesis was identified from an EMS mutant screening for defects in root length, and was used to investigate the role of BR in root development in Arabidopsis.	Brassinosteroids	35-37	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	8-14
29324765-4	2018	Ethylene synthesis is highly increased in the det2-9 mutant compared with the wild type, resulting in the hyper-accumulation of ethylene and the consequent inhibition of root growth.	ethylene	0-8	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	46-52
29324765-4	2018	Ethylene synthesis is highly increased in the det2-9 mutant compared with the wild type, resulting in the hyper-accumulation of ethylene and the consequent inhibition of root growth.	ethylene	128-136	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	46-52
29324765-5	2018	The short-root phenotype of det2-9 was partially recovered in the det2-9/acs9 double mutant and det2-9/ein3/eil1-1 triple mutant which have defects either in ethylene synthesis or ethylene signaling, respectively.	ethylene	158-166	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	28-34
29324765-5	2018	The short-root phenotype of det2-9 was partially recovered in the det2-9/acs9 double mutant and det2-9/ein3/eil1-1 triple mutant which have defects either in ethylene synthesis or ethylene signaling, respectively.	ethylene	180-188	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	28-34
29324765-8	2018	In addition, the det2-9 mutant displayed over accumulated superoxide anions (O2-) compared with the wild-type control, and the increased O2- level was shown to contribute to the inhibition of root growth.	Superoxides	58-75	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	17-23
29324765-8	2018	In addition, the det2-9 mutant displayed over accumulated superoxide anions (O2-) compared with the wild-type control, and the increased O2- level was shown to contribute to the inhibition of root growth.	Superoxides	77-79	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	17-23
29324765-8	2018	In addition, the det2-9 mutant displayed over accumulated superoxide anions (O2-) compared with the wild-type control, and the increased O2- level was shown to contribute to the inhibition of root growth.	Superoxides	137-139	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	17-23
31344072-2	2019	Enzymes DET2 and CYP90 family are responsible for BR biosynthesis in seed plants.	Brassinosteroids	50-52	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	8-12
22170941-5	2012	Abnormal growth of det2 and dwf4 was restored via the application of 28-norcastasterone and closer precursors.	28-norcastasterone	69-87	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	19-23
22822057-6	2012	Enzyme kinetic data of CYP90A1/CPD and DET2, together with those of the earlier studied CYP90B1, CYP90C1, and CYP90D1, suggest that BR biosynthesis proceeds mainly via the campestanol-independent pathway.	Brassinosteroids	132-134	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	39-43
22822057-6	2012	Enzyme kinetic data of CYP90A1/CPD and DET2, together with those of the earlier studied CYP90B1, CYP90C1, and CYP90D1, suggest that BR biosynthesis proceeds mainly via the campestanol-independent pathway.	campestanol	172-183	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	39-43
20169349-3	2010	Triple knock-down of the DET2, DWF4, and SMT2 enzymes also resulted in reduction of brassinosteroid (BR)-specific biosynthesis intermediates.	Brassinosteroids	84-99	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	25-29
20685730-2	2010	In Arabidopsis, BR biosynthetic det2 mutants exhibited delayed flowering time by at least 10 d compared with the wild type.	Brassinosteroids	16-18	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	32-36
20685730-3	2010	The levels of endogenous BRs in det2 were below 10% of the wild type.	Brassinosteroids	25-28	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	32-36
20169349-3	2010	Triple knock-down of the DET2, DWF4, and SMT2 enzymes also resulted in reduction of brassinosteroid (BR)-specific biosynthesis intermediates.	Brassinosteroids	101-103	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	25-29
18045813-8	2008	The TDE1 gene was found to be identical to the DE-ETIOLATED 2 gene known to be involved in brassinosteroid (BR) biosynthesis, and the tde1 probacula-defective phenotypes were recovered in the presence of BR application.	Brassinosteroids	91-106	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	47-61
20035956-6	2010	AtEXPA5 expression was reduced in a brassinosteroid-deficient mutant (det2) and a signaling mutant (bri1-301), while it was increased in bzr1-1D, a dominant mutant of a brassinosteroid transcription factor.	Brassinosteroids	36-51	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	70-74
19526717-5	2009	Our experiments showed that the BR-deficient and BR-insensitive Arabidopsis mutants det2, bri1-5 and bri1-9 were more sensitive to ABA than the wild type (Ws-2), especially the det2 and bri1-9 mutants.	Brassinosteroids	32-34	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	84-88
19526717-5	2009	Our experiments showed that the BR-deficient and BR-insensitive Arabidopsis mutants det2, bri1-5 and bri1-9 were more sensitive to ABA than the wild type (Ws-2), especially the det2 and bri1-9 mutants.	Brassinosteroids	32-34	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	177-181
19526717-5	2009	Our experiments showed that the BR-deficient and BR-insensitive Arabidopsis mutants det2, bri1-5 and bri1-9 were more sensitive to ABA than the wild type (Ws-2), especially the det2 and bri1-9 mutants.	Brassinosteroids	49-51	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	84-88
19526717-5	2009	Our experiments showed that the BR-deficient and BR-insensitive Arabidopsis mutants det2, bri1-5 and bri1-9 were more sensitive to ABA than the wild type (Ws-2), especially the det2 and bri1-9 mutants.	Brassinosteroids	49-51	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	177-181
19526717-5	2009	Our experiments showed that the BR-deficient and BR-insensitive Arabidopsis mutants det2, bri1-5 and bri1-9 were more sensitive to ABA than the wild type (Ws-2), especially the det2 and bri1-9 mutants.	Abscisic Acid	131-134	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	84-88
19526717-5	2009	Our experiments showed that the BR-deficient and BR-insensitive Arabidopsis mutants det2, bri1-5 and bri1-9 were more sensitive to ABA than the wild type (Ws-2), especially the det2 and bri1-9 mutants.	Abscisic Acid	131-134	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	177-181
18045813-8	2008	The TDE1 gene was found to be identical to the DE-ETIOLATED 2 gene known to be involved in brassinosteroid (BR) biosynthesis, and the tde1 probacula-defective phenotypes were recovered in the presence of BR application.	Brassinosteroids	108-110	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	47-61
18045813-8	2008	The TDE1 gene was found to be identical to the DE-ETIOLATED 2 gene known to be involved in brassinosteroid (BR) biosynthesis, and the tde1 probacula-defective phenotypes were recovered in the presence of BR application.	Brassinosteroids	204-206	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	47-61
14617096-5	2003	These results indicate that Uzukobito had a defect in the conversion of (24R)-24-methylcholest-4-en-3-one and (22S, 24R)-22-hydroxy-24-methylcholest-4-en-3-one to their 5alpha-reduced forms, which is catalyzed by de-etiolated2 (DET2) in Arabidopsis.	campest-4-en-3-one	72-105	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	213-226
17565582-2	2007	DET2, an Arabidopsis steroid 5d-reductase, is considered to catalyze a major rate-limiting in brassinosteroid (BR) biosynthesis.	Brassinosteroids	94-109	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	0-4
17565582-2	2007	DET2, an Arabidopsis steroid 5d-reductase, is considered to catalyze a major rate-limiting in brassinosteroid (BR) biosynthesis.	Brassinosteroids	111-113	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	0-4
15993049-5	2005	Similar results were obtained with the expression in COS-7 of recombinant AtDET2 that showed 5alphaR activity for progesterone and not for campestenone.	carbonyl sulfide	53-56	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	74-80
15993049-5	2005	Similar results were obtained with the expression in COS-7 of recombinant AtDET2 that showed 5alphaR activity for progesterone and not for campestenone.	Progesterone	114-126	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	74-80
15993049-9	2005	The presence of two 5alphaR isoenzymes was demonstrated also in Arabidopsis using the det2-1 mutant, in which a residual 5alphaR activity for campestenone and progesterone was evidenced and characterized.	campestenone	142-154	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	86-90
15993049-9	2005	The presence of two 5alphaR isoenzymes was demonstrated also in Arabidopsis using the det2-1 mutant, in which a residual 5alphaR activity for campestenone and progesterone was evidenced and characterized.	Progesterone	159-171	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	86-90
15908602-3	2005	Five BR-specific biosynthesis genes (DET2, DWF4, CPD, BR6ox1, and ROT3) and two sterol biosynthesis genes (FK and DWF5) were up-regulated in BR-depleted wild-type plants grown under brassinazole, a BR biosynthesis inhibitor.	Brassinosteroids	5-7	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	37-41
15908602-3	2005	Five BR-specific biosynthesis genes (DET2, DWF4, CPD, BR6ox1, and ROT3) and two sterol biosynthesis genes (FK and DWF5) were up-regulated in BR-depleted wild-type plants grown under brassinazole, a BR biosynthesis inhibitor.	Brassinosteroids	54-56	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	37-41
16492480-10	2005	Following the isolation of det2 and cpd, a great number of BR-deficient mutants were identified.	Brassinosteroids	59-61	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	27-31
14617096-5	2003	These results indicate that Uzukobito had a defect in the conversion of (24R)-24-methylcholest-4-en-3-one and (22S, 24R)-22-hydroxy-24-methylcholest-4-en-3-one to their 5alpha-reduced forms, which is catalyzed by de-etiolated2 (DET2) in Arabidopsis.	campest-4-en-3-one	72-105	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	228-232
14617096-5	2003	These results indicate that Uzukobito had a defect in the conversion of (24R)-24-methylcholest-4-en-3-one and (22S, 24R)-22-hydroxy-24-methylcholest-4-en-3-one to their 5alpha-reduced forms, which is catalyzed by de-etiolated2 (DET2) in Arabidopsis.	(22s, 24r)-22-hydroxy-24-methylcholest-4-en-3-one	110-159	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	213-226
14617096-5	2003	These results indicate that Uzukobito had a defect in the conversion of (24R)-24-methylcholest-4-en-3-one and (22S, 24R)-22-hydroxy-24-methylcholest-4-en-3-one to their 5alpha-reduced forms, which is catalyzed by de-etiolated2 (DET2) in Arabidopsis.	(22s, 24r)-22-hydroxy-24-methylcholest-4-en-3-one	110-159	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	228-232
12602867-9	2003	Proline accumulation and induction of P5CS1 transcription are simultaneously enhanced in the ABA-hypersensitive prl1 and brassinosteroid-deficient det2 mutants, whereas P5CS2 shows enhanced induction by ABA and salt only in the det2 mutant.	Proline	0-7	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	147-151
12376657-5	2002	Analysis of endogenous levels of these brassinosteroids revealed that det2 accumulates 22-OH-4-en-3-one.	Brassinosteroids	39-55	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	70-74
12602867-9	2003	Proline accumulation and induction of P5CS1 transcription are simultaneously enhanced in the ABA-hypersensitive prl1 and brassinosteroid-deficient det2 mutants, whereas P5CS2 shows enhanced induction by ABA and salt only in the det2 mutant.	Brassinosteroids	121-136	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	147-151
12602867-10	2003	In comparison, the prl1 mutation reduces the basal level of PDH expression, whereas the det2 mutation enhances the inhibition of PDH by ABA.	Abscisic Acid	136-139	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	88-92
12376657-5	2002	Analysis of endogenous levels of these brassinosteroids revealed that det2 accumulates 22-OH-4-en-3-one.	22-oh-4-en-3-one	87-103	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	70-74
12376657-7	2002	Exogenously applied 22-OH-3-one and 6-deoxoCT were found to rescue det2 mutant phenotypes, whereas 22-OHCR and 22-OH-4-en-3-one did not.	22-oh-3-one	20-31	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	67-71
12376657-7	2002	Exogenously applied 22-OH-3-one and 6-deoxoCT were found to rescue det2 mutant phenotypes, whereas 22-OHCR and 22-OH-4-en-3-one did not.	6-deoxocathasterone	36-45	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	67-71
12376657-8	2002	These results substantiate the existence of a new subpathway (22-OHCR --> 22-OH-4-en-3-one --> 22-OH-3-one --> 6-deoxoCT) and reveal that the det2 mutant is defective in the conversion of 22-OH-4-en-3-one to 22-OH-3-one, which leads to brassinolide biosynthesis.	22-ohcr --	62-72	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	151-155
12376657-8	2002	These results substantiate the existence of a new subpathway (22-OHCR --> 22-OH-4-en-3-one --> 22-OH-3-one --> 6-deoxoCT) and reveal that the det2 mutant is defective in the conversion of 22-OH-4-en-3-one to 22-OH-3-one, which leads to brassinolide biosynthesis.	22-oh-4-en-3-one	77-93	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	151-155
12376657-8	2002	These results substantiate the existence of a new subpathway (22-OHCR --> 22-OH-4-en-3-one --> 22-OH-3-one --> 6-deoxoCT) and reveal that the det2 mutant is defective in the conversion of 22-OH-4-en-3-one to 22-OH-3-one, which leads to brassinolide biosynthesis.	22-oh-3-one --	101-115	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	151-155
12376657-8	2002	These results substantiate the existence of a new subpathway (22-OHCR --> 22-OH-4-en-3-one --> 22-OH-3-one --> 6-deoxoCT) and reveal that the det2 mutant is defective in the conversion of 22-OH-4-en-3-one to 22-OH-3-one, which leads to brassinolide biosynthesis.	6-deoxocathasterone	120-129	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	151-155
12376657-8	2002	These results substantiate the existence of a new subpathway (22-OHCR --> 22-OH-4-en-3-one --> 22-OH-3-one --> 6-deoxoCT) and reveal that the det2 mutant is defective in the conversion of 22-OH-4-en-3-one to 22-OH-3-one, which leads to brassinolide biosynthesis.	22-oh-4-en-3-one	197-213	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	151-155
12376657-8	2002	These results substantiate the existence of a new subpathway (22-OHCR --> 22-OH-4-en-3-one --> 22-OH-3-one --> 6-deoxoCT) and reveal that the det2 mutant is defective in the conversion of 22-OH-4-en-3-one to 22-OH-3-one, which leads to brassinolide biosynthesis.	brassinolide	245-257	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	151-155
9401120-0	1997	The Arabidopsis deetiolated2 mutant is blocked early in brassinosteroid biosynthesis.	Brassinosteroids	56-71	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	16-28
12114578-8	2002	Both show a mild phenotype in comparison with BR-deficient mutants such as cpd/cbb3, det2, and dwf4.	Brassinosteroids	46-48	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	85-89
10398719-0	1999	Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-En-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis.	campest-4-en-3-one	51-84	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	12-16
10398719-0	1999	Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-En-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis.	5alpha-campestan-3-one	88-126	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	12-16
10398719-0	1999	Arabidopsis det2 is defective in the conversion of (24R)-24-methylcholest-4-En-3-one to (24R)-24-methyl-5alpha-cholestan-3-one in brassinosteroid biosynthesis.	Brassinosteroids	130-145	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	12-16
10398719-1	1999	Previously, we have shown that the Arabidopsis det2 (deetiolated2) mutant is defective in the biosynthesis of brassinosteroids (BR) and that DET2 (a steroid 5alpha-reductase) acts early in the proposed BR biosynthetic pathway.	Brassinosteroids	110-126	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	47-51
10398719-1	1999	Previously, we have shown that the Arabidopsis det2 (deetiolated2) mutant is defective in the biosynthesis of brassinosteroids (BR) and that DET2 (a steroid 5alpha-reductase) acts early in the proposed BR biosynthetic pathway.	Brassinosteroids	128-130	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	47-51
10398719-4	1999	The results of these studies indicate the early operating steps of BR biosynthesis as: campesterol --> 4-en-3beta-ol --> 4-en-3-one --> 3-one --> campestanol in Arabidopsis, with det2 deficient in the conversion of 4-en-3-one to 3-one.	Brassinosteroids	67-69	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	191-195
10398719-4	1999	The results of these studies indicate the early operating steps of BR biosynthesis as: campesterol --> 4-en-3beta-ol --> 4-en-3-one --> 3-one --> campestanol in Arabidopsis, with det2 deficient in the conversion of 4-en-3-one to 3-one.	campesterol	87-98	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	191-195
10398719-4	1999	The results of these studies indicate the early operating steps of BR biosynthesis as: campesterol --> 4-en-3beta-ol --> 4-en-3-one --> 3-one --> campestanol in Arabidopsis, with det2 deficient in the conversion of 4-en-3-one to 3-one.	Testosterone	127-137	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	191-195
10398719-4	1999	The results of these studies indicate the early operating steps of BR biosynthesis as: campesterol --> 4-en-3beta-ol --> 4-en-3-one --> 3-one --> campestanol in Arabidopsis, with det2 deficient in the conversion of 4-en-3-one to 3-one.	3-one	132-137	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	191-195
10398719-4	1999	The results of these studies indicate the early operating steps of BR biosynthesis as: campesterol --> 4-en-3beta-ol --> 4-en-3-one --> 3-one --> campestanol in Arabidopsis, with det2 deficient in the conversion of 4-en-3-one to 3-one.	3-one	145-150	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	191-195
11867704-3	2002	When the leaves of brassinosteroid-related mutants, det2 (de-etiolated2 = cro1) and dwf1 (dwarf1 = cro2) were compared to wild-type plants, an earlier cessation of leaf expansion was observed; a detailed anatomical analysis further revealed that the mutants had fewer cells per leaf blade.	Brassinosteroids	19-34	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	52-56
11867704-3	2002	When the leaves of brassinosteroid-related mutants, det2 (de-etiolated2 = cro1) and dwf1 (dwarf1 = cro2) were compared to wild-type plants, an earlier cessation of leaf expansion was observed; a detailed anatomical analysis further revealed that the mutants had fewer cells per leaf blade.	Brassinosteroids	19-34	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	58-71
11867704-4	2002	Treatment of the det2 mutants with the brassinosteroid, brassinolide, reversed the mutation and restored the potential for growth to that of the wild type.	Brassinosteroids	39-54	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	17-21
11867704-4	2002	Treatment of the det2 mutants with the brassinosteroid, brassinolide, reversed the mutation and restored the potential for growth to that of the wild type.	brassinolide	56-68	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	17-21
12324600-3	1991	det2 mutations result in dark-grown Arabidopsis thaliana seedlings with many characteristics of light-grown plants, including hypocotyl growth inhibition, cotyledon expansion, primary leaf initiation, anthocyanin accumulation, and derepression of light-regulated gene expression.	Anthocyanins	201-212	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	0-4
8602526-3	1996	A mutation of glutamate 204, which is absolutely required for the activity of human steroid reductase, abolishes the in vivo activity of DET2 and leads to defects in light-regulated development that can be ameliorated by application of a plant steroid, brassinolide.	Glutamic Acid	14-23	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	137-141
8602526-3	1996	A mutation of glutamate 204, which is absolutely required for the activity of human steroid reductase, abolishes the in vivo activity of DET2 and leads to defects in light-regulated development that can be ameliorated by application of a plant steroid, brassinolide.	Steroids	84-91	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	137-141
8602526-3	1996	A mutation of glutamate 204, which is absolutely required for the activity of human steroid reductase, abolishes the in vivo activity of DET2 and leads to defects in light-regulated development that can be ameliorated by application of a plant steroid, brassinolide.	brassinolide	253-265	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	137-141
8602526-4	1996	Thus, DET2 may encode a reductase in the brassinolide biosynthetic pathway, and brassinosteroids may constitute a distinct class of phytohormones with an important role in light-regulated development of higher plants.	brassinolide	41-53	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	6-10
32487564-3	2020	Etiolated seedlings of the brassinosteroids-deficient det2-1 (de-etiolated2) mutant accumulated excess protochlorophyllide, resulting in photo-oxidative damage upon exposure to light.	Brassinosteroids	27-43	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	62-75
32487564-3	2020	Etiolated seedlings of the brassinosteroids-deficient det2-1 (de-etiolated2) mutant accumulated excess protochlorophyllide, resulting in photo-oxidative damage upon exposure to light.	Protochlorophyllide	103-122	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	62-75
31983879-5	2019	We characterized the two genes, SlS5alphaR1 and SlS5alphaR2, which show high homology with DET2, a brassinosteroid 5alpha reductase of Arabidopsis thaliana.	Brassinosteroids	99-114	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	91-95
33980131-1	2021	The increased level of endogenous abscisic acid (ABA) in brassinosteroid (BR)-deficient mutants, such as det2 and cyp85a1 x cyp85a2, suggests that ABA synthesis is inhibited by endogenous BRs in Arabidopsis thaliana.	Abscisic Acid	34-47	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	105-109
33980131-1	2021	The increased level of endogenous abscisic acid (ABA) in brassinosteroid (BR)-deficient mutants, such as det2 and cyp85a1 x cyp85a2, suggests that ABA synthesis is inhibited by endogenous BRs in Arabidopsis thaliana.	Abscisic Acid	49-52	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	105-109
33980131-2	2021	Expression of the ABA biosynthesis gene ABA-deficient 2 (ABA2) was negatively regulated by exogenously applied BR but up-regulated by the application of brassinazole and in det2 and cyp85a1 x cyp85a2.	Abscisic Acid	18-21	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	173-177
33980131-2	2021	Expression of the ABA biosynthesis gene ABA-deficient 2 (ABA2) was negatively regulated by exogenously applied BR but up-regulated by the application of brassinazole and in det2 and cyp85a1 x cyp85a2.	Abscisic Acid	40-43	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	Arabidopsis thaliana	173-177