PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 34050352-11 2021 Exposure to glutamine deprivation increased the level of LC3B, GCN2, ATG12 and beclin-1. Glutamine 12-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 57-61 33747191-9 2021 With the increase in 25-(OH)D3 concentration, the expression level of mTOR increased, the expression level of LC3B-II decreased and the apoptosis rate was significantly increased. Calcifediol 21-30 microtubule associated protein 1 light chain 3 beta Homo sapiens 110-114 34032339-7 2021 CQ inhibited the degradation of mitophagosomes in vitrified oocytes, manifested as decreased mitochondria-lysosomes colocalization, increased fluorescence fraction of VDAC1 overlapping LC3B, increased LC3B-II/I protein expression ratio, and p62 accumulation. Chloroquine 0-2 microtubule associated protein 1 light chain 3 beta Homo sapiens 185-189 33335289-1 2021 Oleate, the most abundant endogenous and dietary cis-unsaturated fatty acid, has the atypical property to cause the redistribution of microtubule-associated proteins 1A/1B light chain 3B (referred to as LC3) to the trans-Golgi network (TGN), as shown here. Oleic Acid 0-6 microtubule associated protein 1 light chain 3 beta Homo sapiens 134-186 33335289-1 2021 Oleate, the most abundant endogenous and dietary cis-unsaturated fatty acid, has the atypical property to cause the redistribution of microtubule-associated proteins 1A/1B light chain 3B (referred to as LC3) to the trans-Golgi network (TGN), as shown here. cis-unsaturated fatty acid 49-75 microtubule associated protein 1 light chain 3 beta Homo sapiens 134-186 32515291-5 2021 Following the combined treatment with piperlongumine and 10 mM N-acetyl-L-cysteine (NAC), intracellular ROS and cell viability returned to normal levels, and the expression of LC3B-II decreased to the predose level. piperlonguminine 38-52 microtubule associated protein 1 light chain 3 beta Homo sapiens 176-180 33705961-6 2021 Further, ceramide accumulation increased mitochondrial fission regulators such as dynamin-related protein 1 (DRP1) and mitochondrial fission factor (MFF) as well as auto/mitophagic proteins LC3B and PINK-1 compared to control. Ceramides 9-17 microtubule associated protein 1 light chain 3 beta Homo sapiens 190-194 33927214-10 2021 Furthermore, the structure of BMI1 and target interaction of AL were virtually screened using the molecular docking program Autodock Vina; AL decreased the expression of N-cadherin, vimentin, and P62 and increased the expression of LC3B and Beclin-1 in xenograft tumors. alantolactone 61-63 microtubule associated protein 1 light chain 3 beta Homo sapiens 232-236 33927214-10 2021 Furthermore, the structure of BMI1 and target interaction of AL were virtually screened using the molecular docking program Autodock Vina; AL decreased the expression of N-cadherin, vimentin, and P62 and increased the expression of LC3B and Beclin-1 in xenograft tumors. alantolactone 139-141 microtubule associated protein 1 light chain 3 beta Homo sapiens 232-236 33920802-10 2021 Consistently, Rh1 treatment significantly reduced tumor growth in vivo and increased the ROS production and protein expression of LC3B and cleaved caspase-3 but decreased the phosphorylation of Akt and retinoblastoma (Rb) in tumor tissues. 2,5-diaziridinyl-3-(hydroxymethyl)-6-methyl-1,4-benzoquinone 14-17 microtubule associated protein 1 light chain 3 beta Homo sapiens 130-134 33539914-0 2021 LC3B/p62-mediated mitophagy protects A549 cells from resveratrol-induced apoptosis. Resveratrol 53-64 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 32515291-5 2021 Following the combined treatment with piperlongumine and 10 mM N-acetyl-L-cysteine (NAC), intracellular ROS and cell viability returned to normal levels, and the expression of LC3B-II decreased to the predose level. Acetylcysteine 63-82 microtubule associated protein 1 light chain 3 beta Homo sapiens 176-180 32515291-5 2021 Following the combined treatment with piperlongumine and 10 mM N-acetyl-L-cysteine (NAC), intracellular ROS and cell viability returned to normal levels, and the expression of LC3B-II decreased to the predose level. Acetylcysteine 84-87 microtubule associated protein 1 light chain 3 beta Homo sapiens 176-180 32515291-6 2021 Gemcitabine also induced cell apoptosis, increased ROS contents, and LC3B-II expression. gemcitabine 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 69-73 33300447-1 2021 Oleate, the most abundantly occurring cis-unsaturated fatty acid, has the particularity to induce the accumulation of MAP1LC3B/LC3 (microtubule associated protein 1 light chain 3 beta) at the trans-Golgi apparatus. Oleic Acid 0-6 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-126 33620066-6 2021 A decrease in LC3B concentration <5.5 ng/ml increased the risk of oxygen and ventilatory requirement (adjusted odds ratio: 4.6; 95% CI: 1.1-22.0; P = 0.04). Oxygen 66-72 microtubule associated protein 1 light chain 3 beta Homo sapiens 14-18 33620066-9 2021 COVID-19 patients with a decrease in LC3B concentration <5.5 ng/ml will require early hospital admission for supplemental oxygen therapy and other respiratory support. Oxygen 122-128 microtubule associated protein 1 light chain 3 beta Homo sapiens 37-41 33243500-4 2021 Besides, Mo or/and Cd elevated the number of autophagosome and microtubule-associated protein light chain 3 (LC3) puncta, upregulated mRNA levels of Beclin-1, LC3A, LC3B, Atg5 and adenosine 5"-monophosphate (AMP)-activated protein kinase alpha1 (AMPKalpha-1), inhibited Dynein, p62 and mammalian target of rapamycin (mTOR) mRNA levels, increased Beclin-1 and LC3II/LC3I protein levels. Cadmium 19-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 165-169 33686057-5 2021 NOX2 residing in the LAPosome membrane generates ROS to cause oxidative inactivation of the protease ATG4B, which otherwise releases LC3B from LAPosomes. Reactive Oxygen Species 49-52 microtubule associated protein 1 light chain 3 beta Homo sapiens 133-137 33300447-1 2021 Oleate, the most abundantly occurring cis-unsaturated fatty acid, has the particularity to induce the accumulation of MAP1LC3B/LC3 (microtubule associated protein 1 light chain 3 beta) at the trans-Golgi apparatus. Oleic Acid 0-6 microtubule associated protein 1 light chain 3 beta Homo sapiens 132-183 33300447-1 2021 Oleate, the most abundantly occurring cis-unsaturated fatty acid, has the particularity to induce the accumulation of MAP1LC3B/LC3 (microtubule associated protein 1 light chain 3 beta) at the trans-Golgi apparatus. cis-unsaturated fatty acid 38-64 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-126 33300447-1 2021 Oleate, the most abundantly occurring cis-unsaturated fatty acid, has the particularity to induce the accumulation of MAP1LC3B/LC3 (microtubule associated protein 1 light chain 3 beta) at the trans-Golgi apparatus. cis-unsaturated fatty acid 38-64 microtubule associated protein 1 light chain 3 beta Homo sapiens 132-183 33388284-6 2021 The CPZ-induced cell death was reversed by the pan-caspase inhibitor Z-VAD-FMK, by the autophagy inhibitor 3-MA and by the knockdown of LC3B using a shRNA (shLC3B), suggesting that autophagy promoted CPZ-induced apoptosis. Chlorpromazine 4-7 microtubule associated protein 1 light chain 3 beta Homo sapiens 136-140 33567124-4 2021 We demonstrated that VviNAC33 induces leaf de-greening, inhibits organ growth and directly activates the expression of STAY-GREEN PROTEIN 1 (SGR1), involved in chlorophyll and photosystem degradation, and AUTOPHAGY 8f (ATG8f) involved in the maturation of autophagosomes. vvinac33 21-29 microtubule associated protein 1 light chain 3 beta Homo sapiens 205-217 33567124-4 2021 We demonstrated that VviNAC33 induces leaf de-greening, inhibits organ growth and directly activates the expression of STAY-GREEN PROTEIN 1 (SGR1), involved in chlorophyll and photosystem degradation, and AUTOPHAGY 8f (ATG8f) involved in the maturation of autophagosomes. vvinac33 21-29 microtubule associated protein 1 light chain 3 beta Homo sapiens 219-224 33577797-7 2021 In turn, the reduced LC3B levels result in slower degradation of the autophagy receptors SQSTM1 and NBR1, and an increased accumulation of puromycin-induced aggresome-like structures. Puromycin 139-148 microtubule associated protein 1 light chain 3 beta Homo sapiens 21-25 33388284-6 2021 The CPZ-induced cell death was reversed by the pan-caspase inhibitor Z-VAD-FMK, by the autophagy inhibitor 3-MA and by the knockdown of LC3B using a shRNA (shLC3B), suggesting that autophagy promoted CPZ-induced apoptosis. Chlorpromazine 200-203 microtubule associated protein 1 light chain 3 beta Homo sapiens 136-140 33501755-9 2021 Autophagy revulsant rapamycin (RAPA) rescued the inhibitory effect of circ-LRP6 on LC3B, vimentin, and Zeb1. Sirolimus 20-29 microtubule associated protein 1 light chain 3 beta Homo sapiens 83-87 32544275-6 2021 Further study showed that resveratrol promoted the mRNA expressions of genes involved in mitophagy, such as transcription factor Ebeta (Tfebeta), Bcl1lc3beta, homo sapiens microtubule-associated protein 1 light chain 3 beta (Map1lc3beta), Parkin, sequestosome1 (p62), autophagy related protein (Atg5) and Beclin-1 (Becn1) in the mammary gland. Resveratrol 26-37 microtubule associated protein 1 light chain 3 beta Homo sapiens 172-223 33501755-9 2021 Autophagy revulsant rapamycin (RAPA) rescued the inhibitory effect of circ-LRP6 on LC3B, vimentin, and Zeb1. Sirolimus 31-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 83-87 33201170-4 2020 This process is blocked by bafilomycin A1 that binds and inhibits the vacuolar ATPase (V-ATPase) and by SopF, a bacterial effector that catalytically modifies the V-ATPase to inhibit LC3B lipidation via ATG16L1. bafilomycin A1 27-41 microtubule associated protein 1 light chain 3 beta Homo sapiens 183-187 33643568-8 2021 Levels of LC3-B, beclin-1, and SIRT1 indicated a significant increase after resveratrol treatment. Resveratrol 76-87 microtubule associated protein 1 light chain 3 beta Homo sapiens 10-15 33643568-10 2021 When the cells were treated with EX527, a SIRT1 inhibitor, the protein contents of LC3-B and Beclin-1 were suppressed. 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide 33-38 microtubule associated protein 1 light chain 3 beta Homo sapiens 83-88 33109567-7 2020 Western blotting assay revealed that melatonin increased the levels of autophagy markers, such as LC-3B and Beclin-1. Melatonin 37-46 microtubule associated protein 1 light chain 3 beta Homo sapiens 98-103 32836151-4 2020 The results demonstrated that Cd exposure notably elevated intracellular and extracellular Cd contents, the number of autophagosomes and LC3 puncta, up-regulated LC3A, LC3B, Beclin-1, Atg5 mRNA levels, and Beclin-1 and LC3II/LC3I protein levels, down-regulated mTOR, p62 and Dynein mRNA levels and p62 protein level. Cadmium 30-32 microtubule associated protein 1 light chain 3 beta Homo sapiens 168-172 33079558-7 2020 Our study demonstrated that the treatment of PmAb-TMZ-PLGA-NPs in GBM tried to adopt the autophagic pathway of the cell survival mechanism with the elevated level of autophagic marker (Beclin-1 and LC3B) at 24 h time point, thereby suppressing the expression of caspase-9 and PARP. Temozolomide 50-53 microtubule associated protein 1 light chain 3 beta Homo sapiens 198-202 33273573-8 2020 Treatment with FeCl3 also decreased expression level of LC-3B, a marker of autophagy in RPH but not in liver-derived cell lines. (1,4,7-trimethyl-1,4,7-triazacyclononane)iron(III) 15-20 microtubule associated protein 1 light chain 3 beta Homo sapiens 56-61 32793311-7 2020 Bafilomycin A1, 3-methyladenine and knockdown of autophagy related (ATG)5 with small interfering RNA prevented erastin-induced breast cancer cell death and inhibited the erastin-induced changes in the expression levels of the autophagy-associated proteins beclin1, ATG5, ATG12, microtubule-associated proteins 1A/1B light chain 3B (LC3B) and P62. erastin 111-118 microtubule associated protein 1 light chain 3 beta Homo sapiens 278-330 33132650-20 2020 Compared with the RA + CD group, the RA + HM group exhibited significantly higher PI3KC1, p-Akt1, and p-mTOR protein levels (P PI3KC1 < 0.01, P p-Akt1 < 0.05, and P p-mTOR < 0.01), a higher p62 protein level (P = 0.057), and significantly lower LC3B and Vps34 protein levels (P < 0.01 for both) in colon tissue. Radium 37-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 245-249 33076962-11 2020 By luciferase assay, MAP1LC3B, a precursor of LC3-II, was confirmed as a direct target of miR-342-3p. lc3-ii 46-52 microtubule associated protein 1 light chain 3 beta Homo sapiens 21-29 33076962-11 2020 By luciferase assay, MAP1LC3B, a precursor of LC3-II, was confirmed as a direct target of miR-342-3p. mir-342-3p 90-100 microtubule associated protein 1 light chain 3 beta Homo sapiens 21-29 33076962-14 2020 The tumor suppressor function of miR-342-3p was mediated via inhibition of pro-survival autophagy by targeting MAP1LC3B and downregulation of DNMT1 with demethylation and re-expression of tumor suppressor genes. mir-342-3p 33-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 111-119 33040385-8 2020 When we assessed native NPY neurones in brain slices from PA-treated animals, we observed increased levels of Atg7 and LC3B protein in response to PA treatment, indicating the induction of autophagy. Palmitates 58-60 microtubule associated protein 1 light chain 3 beta Homo sapiens 119-123 33040385-8 2020 When we assessed native NPY neurones in brain slices from PA-treated animals, we observed increased levels of Atg7 and LC3B protein in response to PA treatment, indicating the induction of autophagy. Palmitates 147-149 microtubule associated protein 1 light chain 3 beta Homo sapiens 119-123 33150055-13 2020 The diffused distribution of pEGFP-C3-MAP1LC3B green fluorescence were become the punctuate pattern fluorescence after treatment with RQDs in cells transfected with pEGFP-C3-MAP1LC3B plasmid RQDs increased the expression of autophagyregulatory proteins LC3 I/II, Beclin-1, p62 and Atg12 in a concentration-dependent manner, similar to autophagy induced by serum starvation, except for p62, as induction of p62 is a characteristic of arsenic compounds. Arsenic 433-440 microtubule associated protein 1 light chain 3 beta Homo sapiens 38-46 33150055-13 2020 The diffused distribution of pEGFP-C3-MAP1LC3B green fluorescence were become the punctuate pattern fluorescence after treatment with RQDs in cells transfected with pEGFP-C3-MAP1LC3B plasmid RQDs increased the expression of autophagyregulatory proteins LC3 I/II, Beclin-1, p62 and Atg12 in a concentration-dependent manner, similar to autophagy induced by serum starvation, except for p62, as induction of p62 is a characteristic of arsenic compounds. Arsenic 433-440 microtubule associated protein 1 light chain 3 beta Homo sapiens 174-182 33132650-19 2020 Compared with those in the RA + CD group, the mRNA expression levels of PI3KC1, Akt1, mTOR, and p62 in the RA + HM group were significantly higher (P PI3KC1 < 0.01 and P Akt1, mTOR, and p62 < 0.05), while those of LC3B were significantly lower (P < 0.05). Radium 107-109 microtubule associated protein 1 light chain 3 beta Homo sapiens 214-218 33085742-4 2020 Our study showed that BIX-01294 (BIX) can induce autophagic vacuoles formation and conversion of LC3B-I to LC3B-II in NPC cells in both dose- and time-dependent manners. BIX 01294 22-31 microtubule associated protein 1 light chain 3 beta Homo sapiens 97-101 33085742-4 2020 Our study showed that BIX-01294 (BIX) can induce autophagic vacuoles formation and conversion of LC3B-I to LC3B-II in NPC cells in both dose- and time-dependent manners. 2-(3,4-dihydroxyphenyl)-2-oxoethyl thiocyanate 22-25 microtubule associated protein 1 light chain 3 beta Homo sapiens 97-101 32793311-7 2020 Bafilomycin A1, 3-methyladenine and knockdown of autophagy related (ATG)5 with small interfering RNA prevented erastin-induced breast cancer cell death and inhibited the erastin-induced changes in the expression levels of the autophagy-associated proteins beclin1, ATG5, ATG12, microtubule-associated proteins 1A/1B light chain 3B (LC3B) and P62. erastin 111-118 microtubule associated protein 1 light chain 3 beta Homo sapiens 332-336 32793311-7 2020 Bafilomycin A1, 3-methyladenine and knockdown of autophagy related (ATG)5 with small interfering RNA prevented erastin-induced breast cancer cell death and inhibited the erastin-induced changes in the expression levels of the autophagy-associated proteins beclin1, ATG5, ATG12, microtubule-associated proteins 1A/1B light chain 3B (LC3B) and P62. erastin 170-177 microtubule associated protein 1 light chain 3 beta Homo sapiens 278-330 32793311-7 2020 Bafilomycin A1, 3-methyladenine and knockdown of autophagy related (ATG)5 with small interfering RNA prevented erastin-induced breast cancer cell death and inhibited the erastin-induced changes in the expression levels of the autophagy-associated proteins beclin1, ATG5, ATG12, microtubule-associated proteins 1A/1B light chain 3B (LC3B) and P62. erastin 170-177 microtubule associated protein 1 light chain 3 beta Homo sapiens 332-336 32793311-8 2020 Furthermore, erastin-induced breast cancer cell death was inhibited by an iron chelator, deferoxamine, which inhibited the increases of erastin-induced iron levels and inhibited the erastin-induced changes in the expression levels of the autophagy-related proteins beclin1, ATG5, ATG12, LC3B and P62. erastin 13-20 microtubule associated protein 1 light chain 3 beta Homo sapiens 287-291 32793311-8 2020 Furthermore, erastin-induced breast cancer cell death was inhibited by an iron chelator, deferoxamine, which inhibited the increases of erastin-induced iron levels and inhibited the erastin-induced changes in the expression levels of the autophagy-related proteins beclin1, ATG5, ATG12, LC3B and P62. Iron 74-78 microtubule associated protein 1 light chain 3 beta Homo sapiens 287-291 32793311-8 2020 Furthermore, erastin-induced breast cancer cell death was inhibited by an iron chelator, deferoxamine, which inhibited the increases of erastin-induced iron levels and inhibited the erastin-induced changes in the expression levels of the autophagy-related proteins beclin1, ATG5, ATG12, LC3B and P62. Deferoxamine 89-101 microtubule associated protein 1 light chain 3 beta Homo sapiens 287-291 32621923-10 2020 A new cellular protective pathway LC3B-DR4/Erk was also disclosed, in which levamisole only increased the expression of LC3B and then activated the phosphorylation of Erk and increased the expression of DR4, while p-Erk and DR4 inter-regulated. Levamisole 76-86 microtubule associated protein 1 light chain 3 beta Homo sapiens 34-38 32966340-8 2020 Concurrently, EPC1 and EPC2 cells underwent autophagy following EtOH exposure, as evidenced by flow cytometry for Cyto-ID, which detects autophagic vesicles, and immunoblots demonstrating induction of the lipidated and cleaved form of LC3B and downregulation of SQSTM1/p62. Ethanol 64-68 microtubule associated protein 1 light chain 3 beta Homo sapiens 235-239 32682846-4 2020 It was shown that zinc significantly induced the level of Beclin1 and LC3B by activating adenosine 5"-monophosphate (AMP)-activated protein kinase (AMPK) signaling pathway. Adenosine 89-98 microtubule associated protein 1 light chain 3 beta Homo sapiens 70-74 32682846-4 2020 It was shown that zinc significantly induced the level of Beclin1 and LC3B by activating adenosine 5"-monophosphate (AMP)-activated protein kinase (AMPK) signaling pathway. Adenosine Monophosphate 117-120 microtubule associated protein 1 light chain 3 beta Homo sapiens 70-74 32971907-8 2020 LC3B, Beclin-1, and ATG5 up-regulation along with autolysosome formation confirmed the induction of autophagy with pimozide treatment. Pimozide 115-123 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 32971907-9 2020 Inhibiting autophagy using 3-methyladenine or LC3B siRNA significantly blocked the apoptosis-inducing effects of pimozide, suggesting that pimozide mediated its apoptotic effects by inducing autophagy. Pimozide 113-121 microtubule associated protein 1 light chain 3 beta Homo sapiens 46-50 32971907-9 2020 Inhibiting autophagy using 3-methyladenine or LC3B siRNA significantly blocked the apoptosis-inducing effects of pimozide, suggesting that pimozide mediated its apoptotic effects by inducing autophagy. Pimozide 139-147 microtubule associated protein 1 light chain 3 beta Homo sapiens 46-50 32621923-10 2020 A new cellular protective pathway LC3B-DR4/Erk was also disclosed, in which levamisole only increased the expression of LC3B and then activated the phosphorylation of Erk and increased the expression of DR4, while p-Erk and DR4 inter-regulated. Levamisole 76-86 microtubule associated protein 1 light chain 3 beta Homo sapiens 120-124 32921987-11 2020 Additionally, the expression levels of Atg7/12/13, beclin and LC3B increased while p-mTOR and P62 expression levels decreased after treatment with fatostatin and tamoxifen. Tamoxifen 162-171 microtubule associated protein 1 light chain 3 beta Homo sapiens 62-66 33041510-5 2020 In this study, we exposed MG-63 cells to different concentrations of quercetin (50, 100 and 200 microM) for 24 h. Here, we show that quercetin increased autophagic flux in the MG-63 cells, as evidenced by the upregulation of LC3B-II/LC3B-I and downregulation of P62/SQSTM1. Quercetin 69-78 microtubule associated protein 1 light chain 3 beta Homo sapiens 225-229 33041510-5 2020 In this study, we exposed MG-63 cells to different concentrations of quercetin (50, 100 and 200 microM) for 24 h. Here, we show that quercetin increased autophagic flux in the MG-63 cells, as evidenced by the upregulation of LC3B-II/LC3B-I and downregulation of P62/SQSTM1. Quercetin 133-142 microtubule associated protein 1 light chain 3 beta Homo sapiens 225-229 32554696-6 2020 Uninfected and HIV-infected macrophages and T cells treated with trehalose exhibited increased markers of autophagy including LC3B lipidation with further accumulation following bafilomycin A1 treatment, and increased levels of LAMP1, LAMP2 and RAB7 proteins required for lysosomal biogenesis and fusion. Trehalose 65-74 microtubule associated protein 1 light chain 3 beta Homo sapiens 126-130 32846959-4 2020 In a dox-induced senescence/polyploidization state, the accumulation of autophagy protein markers, such as LC3B II and p62/SQSTM1, was observed. Doxorubicin 5-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 107-111 32895156-12 2020 The expression of autophagy-related protein light chain 3B (LC3B) was significantly enhanced after HS-10296 treatment (P < 0.01), which also resulted in inhibited phosphorylation of EGFR and AKT proteins in the cells. Almonertinib 99-107 microtubule associated protein 1 light chain 3 beta Homo sapiens 44-58 32774163-9 2020 When tanespimycin was combined with autophage inhibitor 3-MA, alpha-tubulin acetylation elevation was apparently, but LC3B-II was attenuated. tanespimycin 5-17 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-122 32774163-9 2020 When tanespimycin was combined with autophage inhibitor 3-MA, alpha-tubulin acetylation elevation was apparently, but LC3B-II was attenuated. 3-methyladenine 56-60 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-122 32785678-10 2020 Trehalose promoted autophagosome formation and autophagic flux, as evidenced by increased production of Beclin1, Atg5, and Atg7, as well as higher LC3B I protein turnover to LC3B II, with decreased protein levels of P62/SQSTM1. Trehalose 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 147-151 32785678-10 2020 Trehalose promoted autophagosome formation and autophagic flux, as evidenced by increased production of Beclin1, Atg5, and Atg7, as well as higher LC3B I protein turnover to LC3B II, with decreased protein levels of P62/SQSTM1. Trehalose 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 174-178 32895156-12 2020 The expression of autophagy-related protein light chain 3B (LC3B) was significantly enhanced after HS-10296 treatment (P < 0.01), which also resulted in inhibited phosphorylation of EGFR and AKT proteins in the cells. Almonertinib 99-107 microtubule associated protein 1 light chain 3 beta Homo sapiens 60-64 32774666-8 2020 Autophagy was stimulated by metformin, and inhibition of autophagy by 3-methyladenine (3-MA) and chloroquine (CQ) or knockdown of Beclin1 and LC3B blocked the protective effects of metformin. Metformin 181-190 microtubule associated protein 1 light chain 3 beta Homo sapiens 142-146 32774677-3 2020 Here, we showed that incubation of human retinal pigment epithelial (RPE) cells with trehalose enhanced the mRNA and protein expressions of TFEB, autophagy genes ATG5 and ATG7, as well as protein expressions of macroautophagy markers, LC3B and p62/SQTM1, and the chaperone-mediated autophagy (CMA) receptor LAMP2. Trehalose 85-94 microtubule associated protein 1 light chain 3 beta Homo sapiens 235-239 32774677-5 2020 Moreover, trehalose upregulated autophagy flux evident by an increase in the endogenous LC3B level, and accumulation of GFP-LC3B puncta and free GFP fragments in GFP-LC3 - expressing cells in the presence of chloroquine. Trehalose 10-19 microtubule associated protein 1 light chain 3 beta Homo sapiens 88-92 32343612-3 2020 In this study, decreased mitochondrial membrane potential and mtDNA content, increased number of autophagosomes were found in the granulosa cells of PCOS patients and the dihydrotestosterone (DHT)-treated KGN cells, with decreased protein level of the autophagy substrate p62 and increased levels of the cellular autophagy markers Beclin 1 and LC3B-II, while the protein levels of PTEN-induced kinase-1 (PINK1) and Parkin were increased and the level of sirtuin 1 (SIRT1) was decreased. Dihydrotestosterone 192-195 microtubule associated protein 1 light chain 3 beta Homo sapiens 344-348 32765083-9 2020 Metformin treatment and mCherry-GFP-LC3B plasmid transfection showed that metformin could induce the autophagic flux. Metformin 74-83 microtubule associated protein 1 light chain 3 beta Homo sapiens 36-40 32343612-3 2020 In this study, decreased mitochondrial membrane potential and mtDNA content, increased number of autophagosomes were found in the granulosa cells of PCOS patients and the dihydrotestosterone (DHT)-treated KGN cells, with decreased protein level of the autophagy substrate p62 and increased levels of the cellular autophagy markers Beclin 1 and LC3B-II, while the protein levels of PTEN-induced kinase-1 (PINK1) and Parkin were increased and the level of sirtuin 1 (SIRT1) was decreased. Dihydrotestosterone 171-190 microtubule associated protein 1 light chain 3 beta Homo sapiens 344-348 32810966-2 2020 Methods: The expressions of Cdc37 and LC3b were investigated in BTZ-resistant MM cell line ANBL-6.BR using quantitative real-time PCR (qRT-PCR) and western blot (WB) analysis. Bortezomib 64-67 microtubule associated protein 1 light chain 3 beta Homo sapiens 38-42 32810966-9 2020 Results: Cdc37 was down-regulated, while autophagy-associated gene LC3b was upregulated in BTZ-resistant cell line ANBL-6.BR. Bortezomib 91-94 microtubule associated protein 1 light chain 3 beta Homo sapiens 67-71 32430398-4 2020 Using cell viability assays, LC3B immunoblots, and live-cell fluorescence microscopy, we report here that in the presence of ruminant serum, exogenously added polyamines are quickly oxidized by the copper-containing bovine serum amine oxidase (BSAO). Polyamines 159-169 microtubule associated protein 1 light chain 3 beta Homo sapiens 29-33 31877229-8 2020 Interestingly, the AMPK activator AICAR, the autophagy inducer rapamycin and the demethylation inhibitor difenoconazole negated CD74 ablation-offered benefit against LPS-induced cardiac dysfunction while the SUV39H1 inhibitor chaetocin or methylation inhibitor 5-AzaC ameliorated LPS-induced GFP-LC3B formation and cardiomyocyte contractile dysfunction. AICA ribonucleotide 34-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 296-300 32319535-6 2020 In addition, cantharidin treatment increased lipid-modified microtubule-associated proteins 1A/1B light chain 3B expression and significantly attenuated sequestosome 1 expression. Cantharidin 13-24 microtubule associated protein 1 light chain 3 beta Homo sapiens 60-112 32565933-10 2020 The results indicated that LC3B protein expression was enhanced by EPI in a concentration-dependent manner, and the protein levels of cleaved caspase-3 and cleaved caspase-9 were higher in the combination group than in the EPI alone group. Epirubicin 67-70 microtubule associated protein 1 light chain 3 beta Homo sapiens 27-31 32565933-10 2020 The results indicated that LC3B protein expression was enhanced by EPI in a concentration-dependent manner, and the protein levels of cleaved caspase-3 and cleaved caspase-9 were higher in the combination group than in the EPI alone group. Epirubicin 223-226 microtubule associated protein 1 light chain 3 beta Homo sapiens 27-31 32895203-13 2020 Gefitinib significantly increased autophagy in A549 and H1975 cells as shown by increased LC3B expressions following the treatment (P < 0.05). Gefitinib 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 90-94 31448673-0 2020 CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis. Glucose 51-58 microtubule associated protein 1 light chain 3 beta Homo sapiens 12-16 31448673-4 2020 By inhibiting AMPK-MTOR-PIK3C3 pathway, high glucose suppresses the CAV-CAVIN-LC3B-mediated autophagic degradation of CAV1; therefore, more CAV1 is accumulated in the cytosol and utilized to form more caveolae in the cell membrane and facilitates the LDL transcytosis across endothelial cells. Glucose 45-52 microtubule associated protein 1 light chain 3 beta Homo sapiens 78-82 31448673-6 2020 Our results reveal that high glucose stimulates LDL transcytosis by a novel CAV1-CAVIN1-LC3B signaling-mediated autophagic degradation pathway. Glucose 29-36 microtubule associated protein 1 light chain 3 beta Homo sapiens 88-92 32414129-5 2020 Treatment of chloroquine retards the degradation of AHR and triggers physical interaction between AHR and LC3B. Chloroquine 13-24 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 32414129-6 2020 Knockdown of LC3B suppresses the chloroquine-mediated increase of AHR. Chloroquine 33-44 microtubule associated protein 1 light chain 3 beta Homo sapiens 13-17 32393385-6 2020 Reduced AMPK expression by siRNA-mediated knockdown of PRKAA1 and PRKAA2 blocked autophagic flux in EOC spheroids as visualized by fluorescence microscopy using the mCherry-eGFP-LC3B reporter. eoc spheroids 100-113 microtubule associated protein 1 light chain 3 beta Homo sapiens 178-182 32319589-7 2020 It was alsp demonstrated that neferine and TRAIL act synergistically to trigger autophagy in PCa cells, as revealed by autophagosome formation, LC3B-II accumulation demonstrated by transmission electron microscopy (TEM) analysis and phosphorylated c-Jun N-terminal kinase (p-JNK) upregulation. neferine 30-38 microtubule associated protein 1 light chain 3 beta Homo sapiens 144-148 32043704-8 2020 H2 O2 enhanced Beclin-1 and LC3B expression, restricted cell viability, and invasion, and meanwhile caused apoptosis. Hydrogen Peroxide 0-5 microtubule associated protein 1 light chain 3 beta Homo sapiens 28-32 31877229-8 2020 Interestingly, the AMPK activator AICAR, the autophagy inducer rapamycin and the demethylation inhibitor difenoconazole negated CD74 ablation-offered benefit against LPS-induced cardiac dysfunction while the SUV39H1 inhibitor chaetocin or methylation inhibitor 5-AzaC ameliorated LPS-induced GFP-LC3B formation and cardiomyocyte contractile dysfunction. difenoconazole 105-119 microtubule associated protein 1 light chain 3 beta Homo sapiens 296-300 32360415-8 2020 Furthermore, ALT-C increased the content of the autophagic markers LC3B and Beclin-1 in the presence of VEGF, which is associated with decreased angiogenesis. alternagin-C 13-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 67-71 32409746-7 2021 The oncolytic mechanism of rClone30-Anh(F) was analyzed by transcriptome analyses, in comparison with rClone30, rClone30-Anh(F) upregulated the expression of ATG5, Beclin 1, and MAP1LC3B, thus activating autophagy and promoting the production of syncytia. rclone30-anh 27-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 178-186 32409746-7 2021 The oncolytic mechanism of rClone30-Anh(F) was analyzed by transcriptome analyses, in comparison with rClone30, rClone30-Anh(F) upregulated the expression of ATG5, Beclin 1, and MAP1LC3B, thus activating autophagy and promoting the production of syncytia. rclone30-anh 112-124 microtubule associated protein 1 light chain 3 beta Homo sapiens 178-186 32081789-4 2020 Furthermore, KE inhibited autophagosome maturation as demonstrated by blocking the degradation of EGFP puncta in HeLa cells stably expressing EGFP-mRFP-LC3B. ke 13-15 microtubule associated protein 1 light chain 3 beta Homo sapiens 152-156 32341332-6 2020 We next confirmed concomitant upregulation of H4k16ac and H3k27me3 in the same promoter regions of the autophagy-related gene LC3B, reinforcing the specific roles for H4k16ac and H3k27me3 in mediating chidamide-induced transcriptional repression of LC3B. N-(2-amino-5-fluorobenzyl)-4-(N-(pyridine-3-acrylyl)aminomethyl)benzamide 201-210 microtubule associated protein 1 light chain 3 beta Homo sapiens 126-130 31989342-5 2020 Here, we discovered that an NO donor, sodium nitroprusside (SNP) induced autophagy in human SH-SY5Y neuroblastoma cells, including activating LC3B and inhibiting p62. Nitroprusside 38-58 microtubule associated protein 1 light chain 3 beta Homo sapiens 142-146 32189173-9 2020 Autophagy related proteins, such as ATG5 (autophagy related 5), ATG12 (autophagy related 12) and LC3B (microtubule-associated protein 1 light chain 3 beta), were also increased in anisomycin or TGF-beta1 treated corneal fibroblasts. Anisomycin 180-190 microtubule associated protein 1 light chain 3 beta Homo sapiens 97-101 32970976-6 2020 PEBCA induced an integrated stress response-and ATF4-mediated increase in LC3B mRNA, whereas POCA blocked autophagic degradation of LC3-II and long-lived proteins in bulk. polyethylbutylcyanoacrylate 0-5 microtubule associated protein 1 light chain 3 beta Homo sapiens 74-78 31952904-11 2020 After adjusting the confounding variables such as drug usage, LC3-B protein (P = 0.041) and LC3II/LC3I ratio (P = 0.044) were found significantly lower in the lung cells of SM-exposed group. Mustard Gas 173-175 microtubule associated protein 1 light chain 3 beta Homo sapiens 62-67 32189173-9 2020 Autophagy related proteins, such as ATG5 (autophagy related 5), ATG12 (autophagy related 12) and LC3B (microtubule-associated protein 1 light chain 3 beta), were also increased in anisomycin or TGF-beta1 treated corneal fibroblasts. Anisomycin 180-190 microtubule associated protein 1 light chain 3 beta Homo sapiens 103-154 32064256-7 2019 A novel methyladenine alteration was observed due to irreversible changes of GATC motif to G(6 mA) TC in the promoter region of Atg7 and LC3B indicated by cleaved genomic-DNA using the N6 methyladenine-sensitive restriction enzyme DpnI. 2-methyladenine 8-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 137-141 32111854-5 2020 The autophagy inhibitor bafilomycin caused a significant decrease in the production of Nrf2, HO-1 and NQO1 compared to DEPs treatment, whereas the Nrf2 activator sulforaphane increased the LC3B (p = 0.020) levels. bafilomycin 24-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 189-193 32111854-5 2020 The autophagy inhibitor bafilomycin caused a significant decrease in the production of Nrf2, HO-1 and NQO1 compared to DEPs treatment, whereas the Nrf2 activator sulforaphane increased the LC3B (p = 0.020) levels. sulforaphane 162-174 microtubule associated protein 1 light chain 3 beta Homo sapiens 189-193 32116708-5 2020 The expressions of autophagy markers (ATG5, ATG7, Beclin-1, LC3 B) and the necroptosis marker RIPK3 increased and another necroptosis marker RIPK1 decreased after the combination treatment of rapamycin and MK-2206, and were accompanied by the morphological characteristics of autophagy and necroptosis. Sirolimus 192-201 microtubule associated protein 1 light chain 3 beta Homo sapiens 60-65 32033497-13 2020 Combined treatment with Tenovin-6 and autophagy inhibitor chloroquine increased the cytotoxic effect by inducing microtubule-associated protein 1 light chain 3B (LC3B)-II accumulation, and by enhancing apoptosis and cell-cycle arrest. Chloroquine 58-69 microtubule associated protein 1 light chain 3 beta Homo sapiens 162-166 31648047-6 2020 Moreover, rifampicin pretreatment not only improved the ratio of LC3-II/LC3-I in rotenone-treated cells, but also increased autolysosomes and decreased autophagosomes in RFP-GFP-LC3B transfected HM cells exposed to rotenone, thus indicating rifampicin improves autophagy flux in rotenone-treated HM cells. Rifampin 10-20 microtubule associated protein 1 light chain 3 beta Homo sapiens 178-182 31919097-0 2020 Use of the LC3B-fusion technique for biochemical and structural studies of proteins involved in the N-degron pathway. Nitrogen 100-101 microtubule associated protein 1 light chain 3 beta Homo sapiens 11-15 31857374-0 2020 NIMA-related kinase 9-mediated phosphorylation of the microtubule-associated LC3B protein at Thr-50 suppresses selective autophagy of p62/sequestosome 1. Threonine 93-96 microtubule associated protein 1 light chain 3 beta Homo sapiens 77-81 31857374-4 2020 LC3B is phosphorylated at Thr-50 within the LDS by serine/threonine kinase (STK) 3 and STK4. Threonine 26-29 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 31857374-6 2020 All three kinases phosphorylated LC3B Thr-50 in vitro A phospho-mimicking substitution of Thr-50 impaired binding of several LIR-containing proteins, such as ATG4B, FYVE, and coiled-coil domain-containing 1 (FYCO1), and autophagy cargo receptors p62/sequestosome 1 (SQSTM1) and neighbor of BRCA1 gene (NBR1). Threonine 38-41 microtubule associated protein 1 light chain 3 beta Homo sapiens 33-37 31857374-6 2020 All three kinases phosphorylated LC3B Thr-50 in vitro A phospho-mimicking substitution of Thr-50 impaired binding of several LIR-containing proteins, such as ATG4B, FYVE, and coiled-coil domain-containing 1 (FYCO1), and autophagy cargo receptors p62/sequestosome 1 (SQSTM1) and neighbor of BRCA1 gene (NBR1). Threonine 90-93 microtubule associated protein 1 light chain 3 beta Homo sapiens 33-37 31857374-8 2020 Of note, the suppression of p62 degradation was mediated by NEK9-mediated phosphorylation of LC3B Thr-50. Threonine 98-101 microtubule associated protein 1 light chain 3 beta Homo sapiens 93-97 31857374-11 2020 Our findings suggest that NEK9 suppresses LC3B-mediated autophagy of p62 by phosphorylating Thr-50 within the LDS of LC3B. Threonine 92-95 microtubule associated protein 1 light chain 3 beta Homo sapiens 42-46 31857374-11 2020 Our findings suggest that NEK9 suppresses LC3B-mediated autophagy of p62 by phosphorylating Thr-50 within the LDS of LC3B. Threonine 92-95 microtubule associated protein 1 light chain 3 beta Homo sapiens 117-121 31987044-0 2020 Cell death induced by the ER stressor thapsigargin involves death receptor 5, a non-autophagic function of MAP1LC3B, and distinct contributions from unfolded protein response components. Thapsigargin 38-50 microtubule associated protein 1 light chain 3 beta Homo sapiens 107-115 31987044-9 2020 PERK, ATF4 and CHOP were required for Tg-induced cell death, but surprisingly acted in parallel rather than as a linear pathway; ATF4 and CHOP were independently required for Tg-mediated upregulation of death receptor 5 and MAP1LC3B proteins, whereas PERK acted via other pathways. Thapsigargin 38-40 microtubule associated protein 1 light chain 3 beta Homo sapiens 224-232 31987044-9 2020 PERK, ATF4 and CHOP were required for Tg-induced cell death, but surprisingly acted in parallel rather than as a linear pathway; ATF4 and CHOP were independently required for Tg-mediated upregulation of death receptor 5 and MAP1LC3B proteins, whereas PERK acted via other pathways. Thapsigargin 175-177 microtubule associated protein 1 light chain 3 beta Homo sapiens 224-232 32064256-7 2019 A novel methyladenine alteration was observed due to irreversible changes of GATC motif to G(6 mA) TC in the promoter region of Atg7 and LC3B indicated by cleaved genomic-DNA using the N6 methyladenine-sensitive restriction enzyme DpnI. 6-methyladenine 185-201 microtubule associated protein 1 light chain 3 beta Homo sapiens 137-141 32064256-10 2019 Furthermore, a constructed Lpg2936 segment in the GFP expression vector was translocated in the host nucleus and successfully induced methyladenine changes in Atg7 and LC3B promoter region and subsequently regulated autophagy in A549 cells independent of infection. 2-methyladenine 134-147 microtubule associated protein 1 light chain 3 beta Homo sapiens 168-172 32277631-9 2020 Western blot assay confirmed the autophagy results which showed glycyrrhizin-treated cells indicated increased expression of Beclin-1, LC3B-I and LC3B-II in a dose-dependent manner. Glycyrrhizic Acid 64-76 microtubule associated protein 1 light chain 3 beta Homo sapiens 135-139 32329644-5 2020 Rapamycin pre-treatment with Naringin showed significant decrease in mTOR phosphorylation and increase in LC3B activation in AGS cells. Sirolimus 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 32329644-5 2020 Rapamycin pre-treatment with Naringin showed significant decrease in mTOR phosphorylation and increase in LC3B activation in AGS cells. naringin 29-37 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 32329644-11 2020 It has also been elucidated that pre-treatment with NAC inhibited mitochondria-LC3B colocalization, where ROS acted as upstream of ERK1/2-p38 MAPKs activation. nac 52-55 microtubule associated protein 1 light chain 3 beta Homo sapiens 79-83 32329644-11 2020 It has also been elucidated that pre-treatment with NAC inhibited mitochondria-LC3B colocalization, where ROS acted as upstream of ERK1/2-p38 MAPKs activation. ros 106-109 microtubule associated protein 1 light chain 3 beta Homo sapiens 79-83 32277665-8 2020 Nootkatone led to an increase of LC3B-II and LC3B-I but also led to inhibition of p62 expression. nootkatone 0-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 33-37 31963684-7 2020 LC3B-GFP/Lysotracker and mRFP-EGFP-LC3B were utilized to show that Rg5 induced autophagosome-lysosome fusion. ginsenoside Rg5 67-70 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 32021285-7 2020 Results: PHLPP and lysosome-associated membrane protein 2 (LAMP2) levels were downregulated in BTZ-resistant MM cells compared with BTZ-sensitive MM cells, accompanied by inactivation of autophagy pathway evaluated by a reduction in Beclin1, Atg5 and LC3B and increase in p62. bortezomib 95-98 microtubule associated protein 1 light chain 3 beta Homo sapiens 251-255 32277651-12 2020 Western blot analysis showed that the expression of LC3B II increased while of p62 decreased remarkably upon treatment with Withaferin-A, suggestive of autophagic cell death. withaferin A 124-136 microtubule associated protein 1 light chain 3 beta Homo sapiens 52-56 30873870-8 2019 Moreover, after pretreatment with N-acetyl-cysteine, Shikonin increased the production of reactive oxygen species that are involved in regulating ER stress-mediated apoptosis and p38-activated autophagy, as evidenced by the reversion of cell viability and apoptosis and a decrease in p-eIF2alpha, CHOP, p-p38, LC3B-II, and Beclin 1 levels. Acetylcysteine 34-51 microtubule associated protein 1 light chain 3 beta Homo sapiens 310-314 30873870-8 2019 Moreover, after pretreatment with N-acetyl-cysteine, Shikonin increased the production of reactive oxygen species that are involved in regulating ER stress-mediated apoptosis and p38-activated autophagy, as evidenced by the reversion of cell viability and apoptosis and a decrease in p-eIF2alpha, CHOP, p-p38, LC3B-II, and Beclin 1 levels. shikonin 53-61 microtubule associated protein 1 light chain 3 beta Homo sapiens 310-314 32512969-6 2019 After H2O2 treatment, the staining area of Beclin 1 and LC3b was increased and the staining was deeper, and after LiCl handling, the staining area of Beclin 1 and LC3b was further increased and the staining was more deeper. Hydrogen Peroxide 6-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 56-60 31839039-8 2019 Western blot showed that GDC-0152 increased LC3B expression in NB4 cells and promoted the conversion of LC3BI to LC3BII; as compared with GDC-0152 (100 ng/ml), GDC-0152 (100 ng/ml) combined with ROS inhibitor YCG063 (10 mumol/L) decreased apoptosis and autophagy (P<0.05). GDC-0152 25-33 microtubule associated protein 1 light chain 3 beta Homo sapiens 44-48 31659150-5 2019 Furthermore, we show that autophagy leads to iron-dependent ferroptosis by degradation of ferritin and induction of transferrin receptor 1 (TfR1) expression, using wild-type and autophagy-deficient cells, BECN1+/- and LC3B-/-. Iron 45-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 218-222 31673803-9 2019 Rather, dexamethasone upregulated LC3B and increased autophagic flux. Dexamethasone 8-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 34-38 32512969-7 2019 The staining area of Beclin 1 and LC3b in 3-MA group was larger than that in control group, but was smaller than that in model group and lithium group, and the staining was lighter. Lithium 137-144 microtubule associated protein 1 light chain 3 beta Homo sapiens 34-38 32512969-6 2019 After H2O2 treatment, the staining area of Beclin 1 and LC3b was increased and the staining was deeper, and after LiCl handling, the staining area of Beclin 1 and LC3b was further increased and the staining was more deeper. Hydrogen Peroxide 6-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 163-167 32512969-6 2019 After H2O2 treatment, the staining area of Beclin 1 and LC3b was increased and the staining was deeper, and after LiCl handling, the staining area of Beclin 1 and LC3b was further increased and the staining was more deeper. Lithium Chloride 114-118 microtubule associated protein 1 light chain 3 beta Homo sapiens 163-167 31492830-9 2019 The anticancer effects of psilostachyin-A were due to the induction of autophagy which was accompanied by enhancement of LC3B II expression. psilostachyin A 26-41 microtubule associated protein 1 light chain 3 beta Homo sapiens 121-125 31517637-13 2019 Moreover, inhibition of autophagy with molecules such as hydroxychloroquine (20 muMu) and 3-methyladenine (5 mM) was identified by the accumulation of p62 and LC3B. Hydroxychloroquine 57-75 microtubule associated protein 1 light chain 3 beta Homo sapiens 159-163 31517637-13 2019 Moreover, inhibition of autophagy with molecules such as hydroxychloroquine (20 muMu) and 3-methyladenine (5 mM) was identified by the accumulation of p62 and LC3B. mumu 80-84 microtubule associated protein 1 light chain 3 beta Homo sapiens 159-163 31517637-13 2019 Moreover, inhibition of autophagy with molecules such as hydroxychloroquine (20 muMu) and 3-methyladenine (5 mM) was identified by the accumulation of p62 and LC3B. 3-methyladenine 90-105 microtubule associated protein 1 light chain 3 beta Homo sapiens 159-163 31612107-8 2019 Meanwhile, fluorescent GFP-RFP-MAP1LC3B puncta associated with autophagy increased under GG treatment, and transmission electron microscopy confirmed the formation of autophagic vesicles. galangin 89-91 microtubule associated protein 1 light chain 3 beta Homo sapiens 31-39 31564833-7 2019 In addition, emodin increased the number of GFP-LC3 puncta in L02 cells and upregulated the expression of LC3B-II compared to those in control cells. Emodin 13-19 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 30786016-7 2019 Treatment of HPV8-CER expressing cells with the autophagy inhibitor Bafilomycin A1 rescued CHK1 expression and led to LC3B accumulation. bafilomycin 68-79 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-122 31443312-12 2019 Furthermore, amiodarone treatment-induced autophagy in HepG2 cells was demonstrated by autophagosome formation associated with increasing LC3B-II, P62, and Beclin-1 expression. Amiodarone 13-23 microtubule associated protein 1 light chain 3 beta Homo sapiens 138-142 31316620-13 2019 XAV-939 attenuated the miR-24-induced decrease of the protein expression of LC3B and Beclin1, and decreased the stimulatory effects of miR-24 mimics on cell viability. XAV939 0-3 microtubule associated protein 1 light chain 3 beta Homo sapiens 76-80 31316620-13 2019 XAV-939 attenuated the miR-24-induced decrease of the protein expression of LC3B and Beclin1, and decreased the stimulatory effects of miR-24 mimics on cell viability. mir-24 23-29 microtubule associated protein 1 light chain 3 beta Homo sapiens 76-80 31315929-9 2019 Finally, we determined ATG3 residue Lys-243 as an LC3B modification site. Lysine 36-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 50-54 31128157-6 2019 The effects of METH on the protein expressions of autophagy-related genes (LC3B and Beclin-1) were evaluated with and without gastrodin. Methamphetamine 15-19 microtubule associated protein 1 light chain 3 beta Homo sapiens 75-79 31128157-9 2019 The present results indicate that METH exposure elevates the protein expression levels of LC3B and Beclin-1 in a dose- and time-dependent manner. Methamphetamine 34-38 microtubule associated protein 1 light chain 3 beta Homo sapiens 90-94 31128157-10 2019 Gastrodin is observed to block the METH-induced upregulation of LC3B and Beclin-1 protein expression significantly. gastrodin 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 64-68 31128157-10 2019 Gastrodin is observed to block the METH-induced upregulation of LC3B and Beclin-1 protein expression significantly. Methamphetamine 35-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 64-68 31340906-8 2019 Treatment of the cells with 1.6 g/L matrine and 10 nmol/L LY294002 resulted in significantly lowered expressions of p-AKT and p-mTOR proteins and increased the expression of light chain 3B (LC 3B), an autophagy-related protein, as compared with those in the control cells (P &lt; 0.05). 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 58-66 microtubule associated protein 1 light chain 3 beta Homo sapiens 174-188 31337282-11 2019 VK2809 stimulated hepatic autophagic flux as evidenced by increased microtubule-associated protein light chain 3-II (LC3B-II), decreased p62 protein levels, activation of AMP-activated protein kinase (AMPK), inhibition of the mammalian target of rapamycin (mTOR) signaling, enhancement of protein levels of ATG5-ATG12, and increased lysosomal protein expression. MB-07811 0-6 microtubule associated protein 1 light chain 3 beta Homo sapiens 117-121 31340906-8 2019 Treatment of the cells with 1.6 g/L matrine and 10 nmol/L LY294002 resulted in significantly lowered expressions of p-AKT and p-mTOR proteins and increased the expression of light chain 3B (LC 3B), an autophagy-related protein, as compared with those in the control cells (P &lt; 0.05). matrine 36-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 174-188 31340906-8 2019 Treatment of the cells with 1.6 g/L matrine and 10 nmol/L LY294002 resulted in significantly lowered expressions of p-AKT and p-mTOR proteins and increased the expression of light chain 3B (LC 3B), an autophagy-related protein, as compared with those in the control cells (P &lt; 0.05). 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 58-66 microtubule associated protein 1 light chain 3 beta Homo sapiens 190-195 31340906-8 2019 Treatment of the cells with 1.6 g/L matrine and 10 nmol/L LY294002 resulted in significantly lowered expressions of p-AKT and p-mTOR proteins and increased the expression of light chain 3B (LC 3B), an autophagy-related protein, as compared with those in the control cells (P &lt; 0.05). matrine 36-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 190-195 31384174-6 2019 In vitro studies confirmed that ovarian cancer cells were more sensitive to cisplatin by inhibiting LC3B. Cisplatin 76-85 microtubule associated protein 1 light chain 3 beta Homo sapiens 100-104 30788651-6 2019 LC3B was up-regulated by combined treatment of zVAD with chloroquine which also revealed that DNA damage was reduced in live cells but enhanced in dead cells indicating the role of autophagy in maintaining cell health. Chloroquine 57-68 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 31413213-9 2019 The expression levels of Beclin 1, LC3B and P62 in osteosarcoma U2-OS cells after the treatment of cisplatin were detected by Western blotting. Cisplatin 99-108 microtubule associated protein 1 light chain 3 beta Homo sapiens 35-39 31329160-8 2019 Remarkably, human recipients of Abx treatment plus OLT (Abx-OLT), despite severe pretransplantation clinical acuity, had higher EP4 and LC3B levels but lower CHOP levels, which coincided with improved hepatocellular function (serum aspartate aminotransferase/serum aspartate aminotransferase [sALT/sAST]) and a decreased incidence of early allograft dysfunction (EAD). CHEMBL369125 32-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 136-140 29879757-7 2019 Light chain 3B (LC3B), a marker of autophagy, was increased in sensitive cells in a dose-dependent manner with AZD1208 treatment, which suggested that the growth inhibition effect of AZD1208 was achieved through autophagy, not apoptosis. AZD1208 111-118 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-14 31006841-10 2019 Moreover, treatment with autophagy-specific inhibitors or stable knockdown of LC3B by shRNA could markedly enhance curcumin-induced apoptosis. Curcumin 115-123 microtubule associated protein 1 light chain 3 beta Homo sapiens 78-82 31024806-9 2019 The LC3B-II/I ratio was significantly reduced by 3-MA treatment and increased by CQ treatment. 3-methyladenine 49-53 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-8 31024806-9 2019 The LC3B-II/I ratio was significantly reduced by 3-MA treatment and increased by CQ treatment. Chloroquine 81-83 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-8 31090350-10 2019 Results: With the increase of hydrocortisone concentration, the protein expression of LC3B-II increased gradually, and the protein expression of p62 decreased gradually ( P<0.01). Hydrocortisone 30-44 microtubule associated protein 1 light chain 3 beta Homo sapiens 86-90 31090350-11 2019 Compared with group with same concentration of hydrocortisone, the protein expression of LC3B-II decreased and the protein expression of p62 increased after the administration of icariin ( P<0.01). Hydrocortisone 47-61 microtubule associated protein 1 light chain 3 beta Homo sapiens 89-93 31090350-11 2019 Compared with group with same concentration of hydrocortisone, the protein expression of LC3B-II decreased and the protein expression of p62 increased after the administration of icariin ( P<0.01). icariin 179-186 microtubule associated protein 1 light chain 3 beta Homo sapiens 89-93 31053160-8 2019 Celastrol increased the formation of autophagosomes, accumulation of LC3B and the expression of p62 protein. celastrol 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 69-73 30335591-6 2019 Trehalose upregulated genes for the TFEB target and regulator Ppargc1a, lysosomal hydrolases and membrane proteins (Ctsb, Gla, Lamp2a, Mcoln1, Tpp1) and several autophagy-related components (Becn1, Atg10, Atg12, Sqstm1/p62, Map1lc3b, Hspb8 and Bag3) mostly in a PPP3- and TFEB-dependent manner. Trehalose 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 224-232 30776154-3 2019 Whole-mount staining of hair follicles (HFs) and surrounding DWAT for the autophagy marker LC3B confirms the increased presence of LC3B+ lipid droplets adjacent to catagen HFs; moreover, DWAT around catagen HFs engages in greater glycerol release compared to DWAT surrounding anagen HFs. Glycerol 230-238 microtubule associated protein 1 light chain 3 beta Homo sapiens 91-95 29879757-7 2019 Light chain 3B (LC3B), a marker of autophagy, was increased in sensitive cells in a dose-dependent manner with AZD1208 treatment, which suggested that the growth inhibition effect of AZD1208 was achieved through autophagy, not apoptosis. AZD1208 111-118 microtubule associated protein 1 light chain 3 beta Homo sapiens 16-20 29879757-7 2019 Light chain 3B (LC3B), a marker of autophagy, was increased in sensitive cells in a dose-dependent manner with AZD1208 treatment, which suggested that the growth inhibition effect of AZD1208 was achieved through autophagy, not apoptosis. AZD1208 183-190 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-14 29879757-7 2019 Light chain 3B (LC3B), a marker of autophagy, was increased in sensitive cells in a dose-dependent manner with AZD1208 treatment, which suggested that the growth inhibition effect of AZD1208 was achieved through autophagy, not apoptosis. AZD1208 183-190 microtubule associated protein 1 light chain 3 beta Homo sapiens 16-20 31057611-15 2019 Interestingly, upon cisplatin treatment of sh-V0a2-cisR, the autophagy initiation proteins (LC3B, ATG7, and Beclin 1) were found upregulated as a stress response compared to the untreated cells. Cisplatin 20-29 microtubule associated protein 1 light chain 3 beta Homo sapiens 92-96 30934990-5 2019 Utilizing mRFP-EGFP-LC3B, LC3B-GFP/Lysotracker and LC3B-GFP/Rab7-RFP, we show that WFA induces autophagosomes-lysosomes fusion. withaferin A 83-86 microtubule associated protein 1 light chain 3 beta Homo sapiens 20-24 30934990-5 2019 Utilizing mRFP-EGFP-LC3B, LC3B-GFP/Lysotracker and LC3B-GFP/Rab7-RFP, we show that WFA induces autophagosomes-lysosomes fusion. withaferin A 83-86 microtubule associated protein 1 light chain 3 beta Homo sapiens 26-30 30934990-5 2019 Utilizing mRFP-EGFP-LC3B, LC3B-GFP/Lysotracker and LC3B-GFP/Rab7-RFP, we show that WFA induces autophagosomes-lysosomes fusion. withaferin A 83-86 microtubule associated protein 1 light chain 3 beta Homo sapiens 26-30 30964170-12 2019 The correlation analysis found a negative correlation between ATG5 and miRNA-153-3p and a positive correlation between ATG5 and LC3B in gefitinib-resistant NSCLC. Gefitinib 136-145 microtubule associated protein 1 light chain 3 beta Homo sapiens 128-132 30964170-8 2019 MiRNA-153-3p was negatively correlated with autophagy activity marker LC3B in gefitinib-resistant NSCLC patients. Gefitinib 78-87 microtubule associated protein 1 light chain 3 beta Homo sapiens 70-74 30664152-8 2019 Finally, overexpression of miR-122 inhibited the expression of AKT1 and LC3B, while overexpression of miR-199 reduced the levels of mTOR and LC3B. mir-199 102-109 microtubule associated protein 1 light chain 3 beta Homo sapiens 141-145 30551543-5 2019 Concomitant treatment of CoQ10 & methotrexate caused improvement in histological picture of the lung and liver tissues, liver function and oxidative stress biomarkers, modulation of autophagy genes [mammalian target of rapamycin (m-TOR), Microtubule-associated proteins 1 A/1B light chain 3 (MAP1LC3B), and Sequestosome 1 ubiquitin-binding protein p62 (p62/SQSTM1)] with simultaneous reduction in High Mobility Group Protein B1 (HMGB1). coenzyme Q10 25-30 microtubule associated protein 1 light chain 3 beta Homo sapiens 296-304 30635359-7 2019 Niclosamide inhibits mTORC1 and ULK1 activities and induces LC3B expression in niclosamide-sensitive cell lines, but not in the niclosamide-resistant cell lines tested. Niclosamide 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 60-64 30635359-7 2019 Niclosamide inhibits mTORC1 and ULK1 activities and induces LC3B expression in niclosamide-sensitive cell lines, but not in the niclosamide-resistant cell lines tested. Niclosamide 79-90 microtubule associated protein 1 light chain 3 beta Homo sapiens 60-64 30365942-11 2019 Cisplatin induced the ratio of LC3B-II/LC3B-I and Beclin-1 expression, and inhibited the p62 expression. Cisplatin 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 31-35 30365942-11 2019 Cisplatin induced the ratio of LC3B-II/LC3B-I and Beclin-1 expression, and inhibited the p62 expression. Cisplatin 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 39-43 30384152-9 2019 Furthermore, resveratrol activated autophagy, as evidenced by the accumulated autophagic puncta formed by lipid bound LC3B in resveratrol-treated cells. Resveratrol 13-24 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-122 30384152-9 2019 Furthermore, resveratrol activated autophagy, as evidenced by the accumulated autophagic puncta formed by lipid bound LC3B in resveratrol-treated cells. Resveratrol 126-137 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-122 30551543-5 2019 Concomitant treatment of CoQ10 & methotrexate caused improvement in histological picture of the lung and liver tissues, liver function and oxidative stress biomarkers, modulation of autophagy genes [mammalian target of rapamycin (m-TOR), Microtubule-associated proteins 1 A/1B light chain 3 (MAP1LC3B), and Sequestosome 1 ubiquitin-binding protein p62 (p62/SQSTM1)] with simultaneous reduction in High Mobility Group Protein B1 (HMGB1). Adenosine Monophosphate 32-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 296-304 30551543-5 2019 Concomitant treatment of CoQ10 & methotrexate caused improvement in histological picture of the lung and liver tissues, liver function and oxidative stress biomarkers, modulation of autophagy genes [mammalian target of rapamycin (m-TOR), Microtubule-associated proteins 1 A/1B light chain 3 (MAP1LC3B), and Sequestosome 1 ubiquitin-binding protein p62 (p62/SQSTM1)] with simultaneous reduction in High Mobility Group Protein B1 (HMGB1). Methotrexate 37-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 296-304 30563138-13 2018 Western blot analysis in the 25 nM triptolide treatment group revealed induction of autophagy as shown by a significant decrease in the levels of autophagy marker p62 (by 0.2-fold p < 0.0001) and with an increase in the levels of LC3B-II (by 8-fold p < 0.05). triptolide 35-45 microtubule associated protein 1 light chain 3 beta Homo sapiens 233-237 30539860-8 2018 Consistently, 3-MA inhibited, while rapamycin facilitated 5-FU-induced expressions of Beclin1 and LC3B. Fluorouracil 58-62 microtubule associated protein 1 light chain 3 beta Homo sapiens 98-102 30391003-8 2018 Autophagosomes, LC3B protein and apoptotic proteins were significantly increased in DHA treated hypoxic HPAECs. artenimol 84-87 microtubule associated protein 1 light chain 3 beta Homo sapiens 16-20 30272344-6 2018 Neuroinflammation of tumor necrosis factor alpha, interleukin (IL)1beta, IL6 levels and autophagy-related proteins microtubule-associated proteins 1A/1B light chain 3B, autophagy related 16 like 1 and autophagy related 7 were downregulated by berberine treatment in hippocampal pyramidal neurons. Berberine 243-252 microtubule associated protein 1 light chain 3 beta Homo sapiens 115-167 30291937-8 2018 Using RFP-GFP-LC3B transfected BEAS-2B cells exposed to CSE, andrographolide was found to impair autophagosome fusion with lysosome, which may account for the moderate increase in activated caspase 3/7 and annexin V levels. andrographolide 61-76 microtubule associated protein 1 light chain 3 beta Homo sapiens 14-18 30038230-4 2018 ER ceramide stimulates phosphatase 2A and thereby the autophagy proteins Ulk, Beclin, Vps34/Phosphatidylinositol 3-kinase, p62, and Lc3B. Ceramides 3-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 132-136 30442917-7 2018 Furthermore, LC3B was significantly correlated with the asymmetry index in the caudate and putamen, as estimated by a semi-quantitative analysis of [18F] N-(3-fluoropropyl)-2beta-carbon ethoxy-3beta-(4-iodophenyl) nortropane (FP-CIT) positron emission tomography (PET). 18f] n-(3-fluoropropyl)-2beta-carbon ethoxy-3beta-(4-iodophenyl) nortropane 149-224 microtubule associated protein 1 light chain 3 beta Homo sapiens 13-17 30291937-7 2018 However, in the presence of CSE, andrographolide was able to simultaneously increase LC3B-II level and enhance antioxidant defense by decreasing oxidative stress and increasing total antioxidant capacity, through upregulation of nuclear Nrf2 via the p62-Nrf2 positive feedback loop. andrographolide 33-48 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 30477228-8 2018 The coexpression of higher MAP1LC3B and SQSTM1 demonstrated a significantly worse disease-specific survival (DSS) and disease-free survival (DFS) in patients with BMSCC and LSCC, but not TSCC. dss 109-112 microtubule associated protein 1 light chain 3 beta Homo sapiens 27-35 30477228-10 2018 Additionally, silencing both MAP1LC3B and SQSTM1 enhanced the cytotoxic effects of paclitaxel in the tumorspheres of BMSCC cells. Paclitaxel 83-93 microtubule associated protein 1 light chain 3 beta Homo sapiens 29-37 30442917-7 2018 Furthermore, LC3B was significantly correlated with the asymmetry index in the caudate and putamen, as estimated by a semi-quantitative analysis of [18F] N-(3-fluoropropyl)-2beta-carbon ethoxy-3beta-(4-iodophenyl) nortropane (FP-CIT) positron emission tomography (PET). 2-carbomethoxy-8-(3-fluoropropyl)-3-(4-iodophenyl)tropane 226-232 microtubule associated protein 1 light chain 3 beta Homo sapiens 13-17 29994802-3 2018 Our findings indicated that ara-C induces autophagy (with alteration of LC3B, p62, and Beclin-1) in the cells; however, targeting autophagy by 3-methyladenine and chloroquine significantly increased caspase-dependent apoptosis and the sub-G1 compartment in ara-C-treated cells. Cytarabine 28-33 microtubule associated protein 1 light chain 3 beta Homo sapiens 72-76 30408071-9 2018 Finally, there was a strong and significant negative correlation between the survival capacity (MAP1LC3B/BECN1) and both maternal vitamin D and placental VDR in the preeclampsia groups. Vitamin D 130-139 microtubule associated protein 1 light chain 3 beta Homo sapiens 96-104 30170097-5 2018 Additionally, acridine orange staining and immunoblotting with LC3B antibody indicated the suppression of doxorubicin induced autophagy flux with methylglyoxal co-treatment. Doxorubicin 106-117 microtubule associated protein 1 light chain 3 beta Homo sapiens 63-67 30349463-9 2018 Deletion of LC3B resulted in defects within the RPE including increased phagosome accumulation, decreased fatty acid oxidation and a subsequent increase in RPE and sub-RPE lipid deposits. Fatty Acids 106-116 microtubule associated protein 1 light chain 3 beta Homo sapiens 12-16 30170097-5 2018 Additionally, acridine orange staining and immunoblotting with LC3B antibody indicated the suppression of doxorubicin induced autophagy flux with methylglyoxal co-treatment. Pyruvaldehyde 146-159 microtubule associated protein 1 light chain 3 beta Homo sapiens 63-67 29778718-9 2018 Moreover, nimbolide induced autophagy signaling by increasing Beclin 1 and LC3B along with decreased p62 and mTOR protein expression. nimbolide 10-19 microtubule associated protein 1 light chain 3 beta Homo sapiens 75-79 30040176-7 2018 During IRI, ROS specifically activate autophagy-related protein (ATG) 7 promoting autophagic flux and the formation of LC3B-positive puncta around mitochondria in primary human LEC. Reactive Oxygen Species 12-15 microtubule associated protein 1 light chain 3 beta Homo sapiens 119-123 30250025-3 2018 Our data demonstrated that UBCS039 induced a time-dependent activation of autophagy in several human tumor cell lines, as evaluated by increased content of the lipidated form of LC3B by western blot and of autophagosomal puncta by microscopy analysis of GFP-LC3. UBCS039 27-34 microtubule associated protein 1 light chain 3 beta Homo sapiens 178-182 30224639-4 2018 Therefore, we wanted to explore the molecular interplay between autophagy, anoikis, and ROS in relation to LC3B. Reactive Oxygen Species 88-91 microtubule associated protein 1 light chain 3 beta Homo sapiens 107-111 30224639-5 2018 We observed enhanced LC3B level along with increased expression of p62 and modulation of other autophagy-related molecules (Atg 3, 5, 7, 12, 16L1 and Beclin1) by inducing oxidative-stress in ovarian cancer cells using a ROS-producing pro-oxidant molecule. Reactive Oxygen Species 220-223 microtubule associated protein 1 light chain 3 beta Homo sapiens 21-25 30224639-8 2018 Moreover, such upregulation of LC3B via ROS enhanced several apoptotic molecules. Reactive Oxygen Species 40-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 31-35 30224639-10 2018 Furthermore, LC3B-dependent apoptosis was decreased by inhibiting ROS, indicating a possible link between ROS, LC3B, and apoptosis. Reactive Oxygen Species 66-69 microtubule associated protein 1 light chain 3 beta Homo sapiens 13-17 30224639-10 2018 Furthermore, LC3B-dependent apoptosis was decreased by inhibiting ROS, indicating a possible link between ROS, LC3B, and apoptosis. Reactive Oxygen Species 66-69 microtubule associated protein 1 light chain 3 beta Homo sapiens 111-115 30224639-10 2018 Furthermore, LC3B-dependent apoptosis was decreased by inhibiting ROS, indicating a possible link between ROS, LC3B, and apoptosis. Reactive Oxygen Species 106-109 microtubule associated protein 1 light chain 3 beta Homo sapiens 13-17 30224639-11 2018 Additionally, ROS-induced enhanced LC3B promoted detachment-induced cell death (anoikis). Reactive Oxygen Species 14-17 microtubule associated protein 1 light chain 3 beta Homo sapiens 35-39 30127910-4 2018 Salidroside induced autophagy, promoted the protein expression of nucleoporin p62 and the microtubule-associated proteins 1A/1B light chain 3B, suppressed phosphoinositide 3-kinase (PI3K) and phosphorylated protein kinase B (p-Akt) expression, inhibited matrix metalloproteinase-9 (MMP-9) expression and increased that of Bcl-2-associated X protein, which functions as an apoptosis regulator in T24 cells. rhodioloside 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 90-142 29485098-8 2018 Results: Atorvastatin reduced MCF-7 cell proliferation in a dose- and time-dependent manner inducing TUNEL-, Beclin-1-, and LC3B-positive cells. Atorvastatin 9-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 124-128 29749471-6 2018 Mutations in S156 and hydrophobic L213 to alanine counteracted the antiapoptotic properties of BCL2L12 and downregulated the activation of microtubule associated protein 1 light chain 3B (LC3B), autophagy-related (ATG)12-ATG5 conjugates and Beclin-1, compared with a BCL2L12 wild-type group. Alanine 42-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 188-192 29970185-13 2018 Azithromycin increased the expressions of DR4, DR5, p62 and LC-3B proteins and potentiated induction of apoptosis by TRAIL. Azithromycin 0-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 60-65 29897944-6 2018 Autophagic flux upon paclitaxel treatment in vitro was assessed by immunoblotting of LC3B-II and quantitative assessment of WIP1 mRNA. Paclitaxel 21-31 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 30197793-8 2018 Silencing of LC3A, LC3B, and TFEB genes in vitro, significantly sensitized cells to radiotherapy and temozolomide (U87: P < 0.01 and < 0.05, respectively; T98: P < 0.01 and < 0.01, respectively). Temozolomide 101-113 microtubule associated protein 1 light chain 3 beta Homo sapiens 19-23 30112107-9 2018 Our data show that LC3B downregulation decreased the number of apoptotic cells after treatment with SSi6, indicating that autophagy is a key initial step on SSi6-induced caspase-independent apoptosis. ssi6 100-104 microtubule associated protein 1 light chain 3 beta Homo sapiens 19-23 30112107-9 2018 Our data show that LC3B downregulation decreased the number of apoptotic cells after treatment with SSi6, indicating that autophagy is a key initial step on SSi6-induced caspase-independent apoptosis. ssi6 157-161 microtubule associated protein 1 light chain 3 beta Homo sapiens 19-23 29970185-16 2018 CONCLUSION: The synergistic antitumor effect of azithromycin and TRAIL mainly relies on the up-regulations of DR4 and DR5, which in turn result from LC-3B-involved autophagy inhibition. Azithromycin 48-60 microtubule associated protein 1 light chain 3 beta Homo sapiens 149-154 29899555-4 2018 Pre-treatment of cells with specific autophagy inhibitor (3-methyladenine) or knockdown of endogenous LC-3B by siRNA significantly abrogates magnolin-induced cell cycle arrest. magnolin 141-149 microtubule associated protein 1 light chain 3 beta Homo sapiens 102-107 29655680-5 2018 PURPOSE: Our previous proteomics analysis revealed that treatment with PA resulted in the upregulation of an autophagy marker, LC3B in melanoma cells. panduratin A 71-73 microtubule associated protein 1 light chain 3 beta Homo sapiens 127-131 29229996-6 2018 RAB37 promotes ATG5-12 interaction with ATG16L1, thus facilitates lipidation of LC3B in a GTP-dependent manner to enhance autophagy. Guanosine Triphosphate 90-93 microtubule associated protein 1 light chain 3 beta Homo sapiens 80-84 29512762-7 2018 The results demonstrated that cisplatin induces apoptotic cell death in A549 cells and triggers an autophagic response, as indicated by increased microtubule-associated protein 1 light chain 3beta (LC3B)-I/II conversion, increased LC3B puncta and autophagosome formation. Cisplatin 30-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 146-196 29512762-7 2018 The results demonstrated that cisplatin induces apoptotic cell death in A549 cells and triggers an autophagic response, as indicated by increased microtubule-associated protein 1 light chain 3beta (LC3B)-I/II conversion, increased LC3B puncta and autophagosome formation. Cisplatin 30-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 198-202 29512762-7 2018 The results demonstrated that cisplatin induces apoptotic cell death in A549 cells and triggers an autophagic response, as indicated by increased microtubule-associated protein 1 light chain 3beta (LC3B)-I/II conversion, increased LC3B puncta and autophagosome formation. Cisplatin 30-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 231-235 29475942-4 2018 Macrophages treated with dactolisib, JQ1, or SF2523 displayed an increase in LC3B lipidation combined with SQSTM1 degradation without inducing increased cell death. dactolisib 25-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 77-81 29475942-5 2018 LC3B-II levels were further increased in the presence of pepstatin A suggesting that these inhibitors induce autophagic flux. pepstatin 57-68 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 29599373-5 2018 PATIENTS AND METHODS: We immunohistochemically studied the expression of LC3B and ESRRA in 56 MIBC samples. 4-METHYL-2-PENTANOL 94-98 microtubule associated protein 1 light chain 3 beta Homo sapiens 73-77 29599373-9 2018 Double-positive LC3B and ESRRA correlated with poor overall survival (p=0.007) and disease-free survival (p=0.001) in MIBC patients. 4-METHYL-2-PENTANOL 118-122 microtubule associated protein 1 light chain 3 beta Homo sapiens 16-20 29599373-10 2018 CONCLUSION: LC3B and ESRRA might be a useful prognostic factor in patients with MIBC. 4-METHYL-2-PENTANOL 80-84 microtubule associated protein 1 light chain 3 beta Homo sapiens 12-16 29501733-9 2018 (2) CsA induced the formation of autophagosomes and up-regulated the expression of Beclin1, LC3B, and the ERK/MAPK pathway in cardiac fibroblasts. Cyclosporine 4-7 microtubule associated protein 1 light chain 3 beta Homo sapiens 92-96 29577663-10 2018 When pretreated with 3-MA, LC3B II formation and the cytotoxicity declined. 3-methyladenine 21-25 microtubule associated protein 1 light chain 3 beta Homo sapiens 27-31 29292088-7 2018 Moreover, early stage apoptosis rate was significantly increased and autophagy protein LC3 B expression level was higher in oocytes treated with Na2S and/or NH4Cl, which might be caused by ROS elevation. sodium sulfide 145-149 microtubule associated protein 1 light chain 3 beta Homo sapiens 87-92 29292088-7 2018 Moreover, early stage apoptosis rate was significantly increased and autophagy protein LC3 B expression level was higher in oocytes treated with Na2S and/or NH4Cl, which might be caused by ROS elevation. Ammonium Chloride 157-162 microtubule associated protein 1 light chain 3 beta Homo sapiens 87-92 29292088-7 2018 Moreover, early stage apoptosis rate was significantly increased and autophagy protein LC3 B expression level was higher in oocytes treated with Na2S and/or NH4Cl, which might be caused by ROS elevation. Reactive Oxygen Species 189-192 microtubule associated protein 1 light chain 3 beta Homo sapiens 87-92 29374705-9 2018 RESULTS: Naftopidil reduced phospho-AKT (Ser473) and altered LC3B. naftopidil 9-19 microtubule associated protein 1 light chain 3 beta Homo sapiens 61-65 29515581-9 2018 Upon LPS + ATP stimulation, IL-1beta was incorporated to an autophagic compartment, as was revealed by its colocalization with LC3B by confocal microscopy. Adenosine Triphosphate 11-14 microtubule associated protein 1 light chain 3 beta Homo sapiens 127-131 28722162-9 2018 In addition, we found that cyanidin promoted Nrf2 and p62 expression and suppressed LC3B expression during chondrogenic stage of MSCs. cyanidin 27-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 84-88 28722162-10 2018 Meanwhile phosphorylation of IkappaBalpha and autophagosome related protein LC3B were inactivated by cyanidin during chondrocyte hypertrophic stage. cyanidin 101-109 microtubule associated protein 1 light chain 3 beta Homo sapiens 76-80 29355734-7 2018 PpIX-PDT also induces the double membrane autophagosome, up-regulates LC3B, Atg7, Beclin-1, and Bcl-2 expression and down-regulates P62 expression. protoporphyrin IX 0-4 microtubule associated protein 1 light chain 3 beta Homo sapiens 70-74 29183837-7 2018 The viability and expression levels of Cyt C and LC3 B in rotenone and melatonin + rotenone-treated Drp1-overexpressed SH-SY5Y cells were analyzed with MTS and western blotting, respectively. Rotenone 58-66 microtubule associated protein 1 light chain 3 beta Homo sapiens 49-54 28817179-7 2018 Autophagy inhibition by 3 methyladenine (3MA) or RNA interference of LC3B resulted in increased levels of cleaved PARP and caspase-3, and the release of cytochrome c from mitochondria into cytosol in the CoCl2 -treated HDPCs. cobaltous chloride 204-209 microtubule associated protein 1 light chain 3 beta Homo sapiens 69-73 29183837-7 2018 The viability and expression levels of Cyt C and LC3 B in rotenone and melatonin + rotenone-treated Drp1-overexpressed SH-SY5Y cells were analyzed with MTS and western blotting, respectively. Rotenone 83-91 microtubule associated protein 1 light chain 3 beta Homo sapiens 49-54 29246220-8 2017 RESULTS: Sal, alone or in combination with anti-tumor agents, increased expression of autophagic biomarkers, including LC3B and Becline-1, suggesting an autophagy induction. rhodioloside 9-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 119-123 29111403-4 2018 Furthermore, Cd induced autophagy, as demonstrated by microtubule-associated protein 1 light chain 3B (LC3B) plasmid DNA transfection and its conversion from LC3-I to the LC3-II form by autophagy inhibitors, via AMP-activated protein kinase (AMPK)-mammalian target of rapamycin (mTOR) signaling. Cadmium 13-15 microtubule associated protein 1 light chain 3 beta Homo sapiens 103-107 29311680-5 2018 Along with less abundance of p62, autophagy markers LC3B and Beclin-1 significantly increased in HK-2 cells exposed to PA. Palmitic Acid 119-121 microtubule associated protein 1 light chain 3 beta Homo sapiens 52-56 29940794-8 2018 Finally, the 2 autophagic genes LC3B and ATG12 were found to be transcriptionally upregulated by downstream ATF4 and DDIT3 in ER stress, which contributed to the SiNP-enhanced autophagosome synthesis. sinp 162-166 microtubule associated protein 1 light chain 3 beta Homo sapiens 32-36 28939554-4 2017 In this study, we found that ABC294640 induces autophagic death instead of apoptosis in a KSHV long-term-infected immortalized endothelial cell-line, TIVE-LTC, but not in uninfected TIVE cells, through the upregulation of LC3B protein. 3-(4-chlorophenyl)-adamantane-1-carboxylic acid (pyridin-4-ylmethyl)amide 29-38 microtubule associated protein 1 light chain 3 beta Homo sapiens 222-226 28974556-3 2017 In this research, we have found that lycorine significantly induces the apoptotic and autophagic capacities of hepatocellular carcinoma (HCC) cells in vitro and in vivo Treatment with specific autophagy inhibitor (3-methyladenine/Bafilomycin A1) or knockdown of LC-3B/Atg5 by siRNA drastically enhances the apoptotic cell death effect by facilitating the switch from autophagy to apoptosis. lycorine 37-45 microtubule associated protein 1 light chain 3 beta Homo sapiens 262-267 31966544-0 2017 Ursodeoxycholic acid induces autophagy via LC3B to suppress hepatocellular carcinoma in vivo and in vitro. Ursodeoxycholic Acid 0-20 microtubule associated protein 1 light chain 3 beta Homo sapiens 43-47 31966544-4 2017 No researches have been published on the relationship of UDCA and LC3B. 2-methyl-3-(2-((4-nitrooxybutyloxy)carbonyl)vinyl)phenyl ursodeoxycholic acid ester 57-61 microtubule associated protein 1 light chain 3 beta Homo sapiens 66-70 28844710-4 2017 Increased expression of autophagy-regulated genes; Plac8, LC3B and Lamp-1; in CTPE cells was associated with cadmium-induced transformation. Cadmium 109-116 microtubule associated protein 1 light chain 3 beta Homo sapiens 58-62 28774732-5 2017 Meanwhile, PB induced autophagosome formation, as characterized by increased acridine orange-stained positive cells, accumulation of punctate LC3B fluorescence and a greater number of autophagic vacuoles under electron microscopy. physapubenolide 11-13 microtubule associated protein 1 light chain 3 beta Homo sapiens 142-146 28774732-7 2017 Depletion of LC3B, ATG5 or ATG7 reduced PB-induced cytotoxicity, indicating that autophagosome associated cell death participated in the anti-cancer effect of PB. physapubenolide 40-42 microtubule associated protein 1 light chain 3 beta Homo sapiens 13-17 28774732-7 2017 Depletion of LC3B, ATG5 or ATG7 reduced PB-induced cytotoxicity, indicating that autophagosome associated cell death participated in the anti-cancer effect of PB. physapubenolide 159-161 microtubule associated protein 1 light chain 3 beta Homo sapiens 13-17 28979680-11 2017 Cisplatin also induced the formation of autophagosomes and increased autophagy-related proteins ATG 5, ATG 7, Beclin 1 and LC3B II/LC3B I. Cisplatin 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 123-127 28979680-11 2017 Cisplatin also induced the formation of autophagosomes and increased autophagy-related proteins ATG 5, ATG 7, Beclin 1 and LC3B II/LC3B I. Cisplatin 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 131-135 28655748-7 2017 Finally, we show that conversion of p62/SQSTM1, FUNDC1 and FIP200 LIRs into our newly defined GIM, by introducing two valine residues, enhances their interaction with endogenous GABARAP over LC3B. Valine 118-124 microtubule associated protein 1 light chain 3 beta Homo sapiens 191-195 28580603-5 2017 In our results, high glucose significantly increased PTEN-induced putative kinase 1 (PINK1) and LC-3B expressions; as well it decreased cytochrome c oxidase subunit 4 expression and Mitotracker fluorescence intensity. Glucose 21-28 microtubule associated protein 1 light chain 3 beta Homo sapiens 96-101 28710282-5 2017 We found that IBTKalpha is located in the ER and associates with proteins LC3b, SEC16A, and SEC31A and plays a previously unrecognized role in phagophore initiation from ER exit sites. ibtkalpha 14-23 microtubule associated protein 1 light chain 3 beta Homo sapiens 74-78 28808307-3 2017 When examining the autophagy pathway as the main route for aggresomes clearance, CCHE-45 cells displayed increased autophagy flux mediated by MAP1LC3B. cche 81-85 microtubule associated protein 1 light chain 3 beta Homo sapiens 142-150 28927033-6 2017 After the RA+TMZ treatment of U251 cells, autophagy associated proteins Beclin 1 and LC3B were significantly increased, and the TEM analysis were consistent with autophagy protein levels. Tretinoin 10-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 28927033-6 2017 After the RA+TMZ treatment of U251 cells, autophagy associated proteins Beclin 1 and LC3B were significantly increased, and the TEM analysis were consistent with autophagy protein levels. Temozolomide 13-16 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 28767736-7 2017 Moreover, CSE-induced aggresome-formation (LC3B-GFP and Ub-RFP co-localization) and autophagy-flux impairment was significantly (p<0.01) mitigated by cysteamine (p<0.05) or fisetin (p<0.05) treatment, indicating the restoration of CSE-mediated autophagy-impairment. Cysteamine 153-163 microtubule associated protein 1 light chain 3 beta Homo sapiens 43-47 27835930-5 2017 Our in vitro results indicate that GEM/fisetin-mediated TFEB induction significantly (p < 0.05) decreases CSE-induced autophagy-impairment (Ub/LC3B reporter and autophagy flux assay) and resulting aggresome-formation (Ub/p62 coexpression/accumulation; immunoblotting and staining) by controlling reactive oxygen species (ROS) activity. fisetin 39-46 microtubule associated protein 1 light chain 3 beta Homo sapiens 146-150 28498472-8 2017 Salinomycin increased the expression of autophagy marker protein, LC3B, and accumulation of acidic vesicular organelles. salinomycin 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 66-70 29163768-4 2017 Depletion of ING4 stimulated the enzymatic activity of PIK3C3, as visualized by means of a red fluorescent protein-tagged short peptide (FYVE) that specifically binds to phosphatidylinositol-3-phosphate (PI3P)-containing subcellular vesicles and enhanced autophagy, as indicated by an enhanced lipidation of microtubule-associated proteins 1A/1B light chain 3 beta (LC3B) and the redistribution of a green-fluorescent protein (GFP)-LC3B fusion protein to cytoplasmic puncta. phosphatidylinositol 3-phosphate 170-202 microtubule associated protein 1 light chain 3 beta Homo sapiens 308-364 29163768-4 2017 Depletion of ING4 stimulated the enzymatic activity of PIK3C3, as visualized by means of a red fluorescent protein-tagged short peptide (FYVE) that specifically binds to phosphatidylinositol-3-phosphate (PI3P)-containing subcellular vesicles and enhanced autophagy, as indicated by an enhanced lipidation of microtubule-associated proteins 1A/1B light chain 3 beta (LC3B) and the redistribution of a green-fluorescent protein (GFP)-LC3B fusion protein to cytoplasmic puncta. phosphatidylinositol 3-phosphate 170-202 microtubule associated protein 1 light chain 3 beta Homo sapiens 366-370 29163768-4 2017 Depletion of ING4 stimulated the enzymatic activity of PIK3C3, as visualized by means of a red fluorescent protein-tagged short peptide (FYVE) that specifically binds to phosphatidylinositol-3-phosphate (PI3P)-containing subcellular vesicles and enhanced autophagy, as indicated by an enhanced lipidation of microtubule-associated proteins 1A/1B light chain 3 beta (LC3B) and the redistribution of a green-fluorescent protein (GFP)-LC3B fusion protein to cytoplasmic puncta. phosphatidylinositol 3-phosphate 170-202 microtubule associated protein 1 light chain 3 beta Homo sapiens 432-436 28650983-6 2017 In addition to its effect on apoptosis, clivorine treatment could promote autophagy in Huh-7.5 cells, as evidenced by the accumulation of autophagosomes, the enhancement of LC3B expression at the concentrations close to its IC0 value, and the increased conversion of LC3B-I to LC3B-II in the presence of lysosomal inhibitor (chloroquine) and decreased formation of green fluorescent protein (GFP)-LC3 positive puncta in the presence of autophagic sequestration inhibitor (3-methyladenine). clivorine 40-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 173-177 28650983-6 2017 In addition to its effect on apoptosis, clivorine treatment could promote autophagy in Huh-7.5 cells, as evidenced by the accumulation of autophagosomes, the enhancement of LC3B expression at the concentrations close to its IC0 value, and the increased conversion of LC3B-I to LC3B-II in the presence of lysosomal inhibitor (chloroquine) and decreased formation of green fluorescent protein (GFP)-LC3 positive puncta in the presence of autophagic sequestration inhibitor (3-methyladenine). clivorine 40-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 267-271 28650983-6 2017 In addition to its effect on apoptosis, clivorine treatment could promote autophagy in Huh-7.5 cells, as evidenced by the accumulation of autophagosomes, the enhancement of LC3B expression at the concentrations close to its IC0 value, and the increased conversion of LC3B-I to LC3B-II in the presence of lysosomal inhibitor (chloroquine) and decreased formation of green fluorescent protein (GFP)-LC3 positive puncta in the presence of autophagic sequestration inhibitor (3-methyladenine). clivorine 40-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 267-271 28659924-8 2017 Intriguingly, we also found that the upregulation of LC3B expression by rapamycin and heat-killed Brucella in vitro inhibits M2 macrophage polarization, which can be reversed partially by 3-MA. 3-methyladenine 188-192 microtubule associated protein 1 light chain 3 beta Homo sapiens 53-57 27624546-16 2017 LC3B knockdown, in turn, significantly decreased butyrate-induced cell death. Butyrates 49-57 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 27624546-17 2017 Therefore, AMPK-dependent LC3B induction apparently plays an important role in butyrate-induced cell death. Butyrates 79-87 microtubule associated protein 1 light chain 3 beta Homo sapiens 26-30 27624546-19 2017 CONCLUSION: Taken together, starvation and butyrate exposure promote autophagy via AMPK signaling, while the histone deacetylase-inhibitory effects of butyrate alter chromatin to transcriptionally active state, resulting in strong LC3B induction and subsequent cell death. Butyrates 151-159 microtubule associated protein 1 light chain 3 beta Homo sapiens 231-235 30460069-4 2017 In this study, we found that an increase of LC3B-II protein expression level and a decrease of the p62 protein expression level in both cells treated by Earle"s balanced salt solution. earle"s balanced salt solution 153-183 microtubule associated protein 1 light chain 3 beta Homo sapiens 44-48 28550286-1 2017 A novel sandwich-assay electrochemical immunosensor for simultaneous determination of autophagic biomarkers was introduced for the first time, the gold-reduced grapheme oxide nanocomposite (Au/r-GO) set as a good conductive platform with super high specific area, and provided more binding sites for the both antibodies of Beclin-1 and LC3B-II. grapheme oxide 160-174 microtubule associated protein 1 light chain 3 beta Homo sapiens 336-340 28531143-11 2017 Furthermore, an increase of autophagy-associated protein microtubule-associated protein-1 light chain-3B (LC3B)-II and the decrease of Beclin-1 and p62/SQSTM1 were observed following 4-HPPP treatment. 4-hppp 183-189 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-114 28903368-3 2017 Functional autophagy was demonstrated in CC cell lines (LoVo; HT-29) showing a dose-dependent increase of the autophagy markers LC3B, p62 and autophagic vesciles upon increasing concentrations of the autophagy inhibitor chloroquine, which was demonstrated by immunoblotting, immunofluorescence and electron microscopy. Chloroquine 220-231 microtubule associated protein 1 light chain 3 beta Homo sapiens 128-132 28131804-5 2017 IFN-lambda1 could also inhibit HCV-induced or tunicamycin (a known inducer of autophagy with similar mechanism to HCV infection) -induced LC3B-II expression and autophagosome formation. Tunicamycin 46-57 microtubule associated protein 1 light chain 3 beta Homo sapiens 138-142 28391030-6 2017 Metformin triggered autophagy (LC3B expression) was identified to interplay with apoptosis to attenuate the drug effect and postpone cancer cell death. Metformin 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 31-35 28260799-13 2017 BMA-155Cl administration effectively suppressed the growth of HepG-2 xenografts in vivo, and increased the protein expression levels of LC3B, Beclin-1, NF-kappaB p65, and Bax in vivo. bma-155cl 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 136-140 28442745-3 2017 Isothermal titration calorimetry and NMR indicate a ~100 fold enhanced affinity of the serine 34/35-phosphorylated Nix LC3-interacting region (LIR) to LC3B and formation of a very rigid complex compared to the non-phosphorylated sequence. Serine 87-93 microtubule associated protein 1 light chain 3 beta Homo sapiens 151-155 28442745-4 2017 Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Peptides 68-75 microtubule associated protein 1 light chain 3 beta Homo sapiens 35-39 28442745-4 2017 Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Peptides 68-75 microtubule associated protein 1 light chain 3 beta Homo sapiens 202-206 28442745-4 2017 Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Peptides 68-75 microtubule associated protein 1 light chain 3 beta Homo sapiens 202-206 28442745-4 2017 Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Glutamates 87-101 microtubule associated protein 1 light chain 3 beta Homo sapiens 35-39 28442745-4 2017 Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Glutamates 87-101 microtubule associated protein 1 light chain 3 beta Homo sapiens 202-206 28442745-4 2017 Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Glutamates 87-101 microtubule associated protein 1 light chain 3 beta Homo sapiens 202-206 28442745-4 2017 Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Hydrogen 247-255 microtubule associated protein 1 light chain 3 beta Homo sapiens 35-39 28442745-4 2017 Moreover, the crystal structure of LC3B in complex with the Nix LIR peptide containing glutamic acids as phosphomimetic residues and NMR experiments revealed that LIR phosphorylation stabilizes the Nix:LC3B complex via formation of two additional hydrogen bonds between phosphorylated serines of Nix LIR and Arg11, Lys49 and Lys51 in LC3B. Serine 285-292 microtubule associated protein 1 light chain 3 beta Homo sapiens 35-39 28103582-2 2017 Treatment of icaritin in both primary and established (HT-29) CRC cells induced feedback activation of autophagy, evidenced by p62 degradation, Beclin-1 and autophagy-related gene-5 (ATG-5) upregulation, as well as light chain 3B (LC3B)-GFP puncta formation. icaritin 13-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 215-229 27200496-4 2017 EGCG induced CAR cell apoptosis and autophagy by 4",6-diamidino-2-phenylindole (DAPI) dye, acridine orange (AO) staining and green fluorescent protein (GFP)-tagged LC3B assay, respectively. epigallocatechin gallate 0-4 microtubule associated protein 1 light chain 3 beta Homo sapiens 164-168 27200496-6 2017 EGCG markedly increased the protein levels of Bax, cleaved caspase-9, cleaved caspase-3, Atg5, Atg7, Atg12, Beclin-1, and LC3B-II, as well as significantly decreased the expression of Bcl-2, phosphorylated AKT (Ser473) and phosphorylation of STAT3 on Tyr705 by western blotting in CAR cells. epigallocatechin gallate 0-4 microtubule associated protein 1 light chain 3 beta Homo sapiens 122-126 28274308-7 2017 Then, T-butyl-4 (BHA), N-acetylcysteine (NAC) and coenzyme Q10 (CoQ10) were used separately to block the ROS and the expression of LC3B in Pg-LPS-treated HGFs was tested by Western blotting. t-butyl-4 6-15 microtubule associated protein 1 light chain 3 beta Homo sapiens 131-135 28274308-7 2017 Then, T-butyl-4 (BHA), N-acetylcysteine (NAC) and coenzyme Q10 (CoQ10) were used separately to block the ROS and the expression of LC3B in Pg-LPS-treated HGFs was tested by Western blotting. Butylated Hydroxyanisole 17-20 microtubule associated protein 1 light chain 3 beta Homo sapiens 131-135 28274308-7 2017 Then, T-butyl-4 (BHA), N-acetylcysteine (NAC) and coenzyme Q10 (CoQ10) were used separately to block the ROS and the expression of LC3B in Pg-LPS-treated HGFs was tested by Western blotting. Acetylcysteine 23-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 131-135 28274308-7 2017 Then, T-butyl-4 (BHA), N-acetylcysteine (NAC) and coenzyme Q10 (CoQ10) were used separately to block the ROS and the expression of LC3B in Pg-LPS-treated HGFs was tested by Western blotting. Acetylcysteine 41-44 microtubule associated protein 1 light chain 3 beta Homo sapiens 131-135 28274308-7 2017 Then, T-butyl-4 (BHA), N-acetylcysteine (NAC) and coenzyme Q10 (CoQ10) were used separately to block the ROS and the expression of LC3B in Pg-LPS-treated HGFs was tested by Western blotting. pg-lps 139-145 microtubule associated protein 1 light chain 3 beta Homo sapiens 131-135 28103582-2 2017 Treatment of icaritin in both primary and established (HT-29) CRC cells induced feedback activation of autophagy, evidenced by p62 degradation, Beclin-1 and autophagy-related gene-5 (ATG-5) upregulation, as well as light chain 3B (LC3B)-GFP puncta formation. icaritin 13-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 231-235 28106723-6 2017 Further research using 3-methyladenine (3-MA), bafilomycin A1, and ad-mCherry-GFP-LC3B adenovirus demonstrated that LC3B-II increase induced by TNF-alpha was attributed to the inhibition of autophagic degradation. 3-methyladenine 23-38 microtubule associated protein 1 light chain 3 beta Homo sapiens 116-120 28099155-6 2017 The underlying mechanisms in this process included a reduction in Src-mediated CEBPD protein degradation and an increase in CEBPD-regulated LC3B and ATG3 gene transcription under metformin treatment. Metformin 179-188 microtubule associated protein 1 light chain 3 beta Homo sapiens 140-144 28075474-9 2017 The effects on autophagy in isomahanine-treated cells were investigated via conversion of LC3B and degradation of p62/SQSTM1 (p62). mahanine 28-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 90-94 28075474-10 2017 Isomahanine obviously induced autophagic flux as shown by an increase in punctate GFP-LC3B and the LC3B-II/LC3B-I ratio with a concomitant decrease in p62 levels. mahanine 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 86-90 28075474-10 2017 Isomahanine obviously induced autophagic flux as shown by an increase in punctate GFP-LC3B and the LC3B-II/LC3B-I ratio with a concomitant decrease in p62 levels. mahanine 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 99-103 28075474-10 2017 Isomahanine obviously induced autophagic flux as shown by an increase in punctate GFP-LC3B and the LC3B-II/LC3B-I ratio with a concomitant decrease in p62 levels. mahanine 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 99-103 28106723-6 2017 Further research using 3-methyladenine (3-MA), bafilomycin A1, and ad-mCherry-GFP-LC3B adenovirus demonstrated that LC3B-II increase induced by TNF-alpha was attributed to the inhibition of autophagic degradation. 3-methyladenine 40-44 microtubule associated protein 1 light chain 3 beta Homo sapiens 116-120 28106723-6 2017 Further research using 3-methyladenine (3-MA), bafilomycin A1, and ad-mCherry-GFP-LC3B adenovirus demonstrated that LC3B-II increase induced by TNF-alpha was attributed to the inhibition of autophagic degradation. bafilomycin 47-58 microtubule associated protein 1 light chain 3 beta Homo sapiens 116-120 27751602-7 2017 In addition, LY294002 (20 muM) significantly inhibited changes in ratio of LC3B-II to LC3B-I, LC3B mRNA transcript levels, and autolysosome formation. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 13-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 75-79 28069066-11 2017 Western blot revealed the enhanced autophagy proteins LC3B and Atg16L1 expression by RSV. Resveratrol 85-88 microtubule associated protein 1 light chain 3 beta Homo sapiens 54-58 26742518-6 2017 Subsequently, EGCG can restore the disrupted autophagy flux after SAH both at the initiation and formation stages by regulating Atg5, LC3B, and Becn-1 (Beclin-1) mRNA expressions. epigallocatechin gallate 14-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 134-138 26822499-9 2017 Tetrandrine triggered LC3B expression and induced autophagy in CAL 27 cells. tetrandrine 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 22-26 27757847-7 2017 Intriguingly, the side chain of LC3B Lys49 shifts remarkably and forms a hydrogen bond and electrostatic interaction with the phosphate group of FUNDC1 pS17. Hydrogen 73-81 microtubule associated protein 1 light chain 3 beta Homo sapiens 32-36 27757847-7 2017 Intriguingly, the side chain of LC3B Lys49 shifts remarkably and forms a hydrogen bond and electrostatic interaction with the phosphate group of FUNDC1 pS17. Phosphates 126-135 microtubule associated protein 1 light chain 3 beta Homo sapiens 32-36 27751602-7 2017 In addition, LY294002 (20 muM) significantly inhibited changes in ratio of LC3B-II to LC3B-I, LC3B mRNA transcript levels, and autolysosome formation. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 13-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 86-90 27751602-7 2017 In addition, LY294002 (20 muM) significantly inhibited changes in ratio of LC3B-II to LC3B-I, LC3B mRNA transcript levels, and autolysosome formation. 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one 13-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 86-90 27337297-5 2016 Furthermore, cocaine treatment resulted in increased formation of endogenous MAP1LC3B puncta in human astrocytes. Cocaine 13-20 microtubule associated protein 1 light chain 3 beta Homo sapiens 77-85 27764541-8 2016 In intact U87MG human glioblastoma cells rotenone-induced autophagy leads to LC3B translocation to mitochondria and subsequent delivery of mitochondria to lysosomes. Rotenone 41-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 77-81 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). phosphatidylethanolamine 88-112 microtubule associated protein 1 light chain 3 beta Homo sapiens 15-67 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). phosphatidylethanolamine 114-116 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-8 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). phosphatidylethanolamine 114-116 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-8 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). phosphatidylethanolamine 114-116 microtubule associated protein 1 light chain 3 beta Homo sapiens 15-67 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). Tryptophan 328-338 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-8 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). Tryptophan 328-338 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-8 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). Tryptophan 328-338 microtubule associated protein 1 light chain 3 beta Homo sapiens 15-67 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). Leucine 343-350 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-8 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). Leucine 343-350 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-8 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). Leucine 343-350 microtubule associated protein 1 light chain 3 beta Homo sapiens 15-67 27670946-8 2016 In the presence of rapamycin, LC3B accumulation was further increased; however, this effect was attenuated by 3-MA. Sirolimus 19-28 microtubule associated protein 1 light chain 3 beta Homo sapiens 30-34 27187184-12 2016 Interestingly, inhibition of LC3B either by ROS inhibitor N-acetyl-l-cysteine (NAC) or adenovirus-mediated LC3B shRNA could partly restore AECII transdifferentiation under hyperoxia condition. Reactive Oxygen Species 44-47 microtubule associated protein 1 light chain 3 beta Homo sapiens 29-33 27337297-4 2016 Our findings demonstrated that cocaine mediated the activation of astrocytes by altering the levels of autophagy markers, such as BECN1, ATG5, MAP1LC3B-II, and SQSTM1 in both human A172 astrocytoma cells and primary human astrocytes. Cocaine 31-38 microtubule associated protein 1 light chain 3 beta Homo sapiens 143-151 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). phosphatidylethanolamine 88-112 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-8 27540684-2 2016 MAP1LC3B/LC3B (microtubule- associated protein 1 light chain 3 beta) is conjugated with phosphatidylethanolamine (PE) in the membranes and regulates initiation of autophagy through interaction with many autophagy-related proteins possessing an LC3-interacting region (LIR) motif, which is composed of 2 hydrophobic amino acids (tryptophan and leucine) separated by 2 non-conserved amino acids (WXXL). phosphatidylethanolamine 88-112 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-8 27514525-7 2016 Further studies demonstrated that after pretreatment with 3-MA (3 mmol/L) for 48 h, siKDM4A-transfected cells showed a prominent decrease in LC3B-II/LC3B-I and Beclin 1, accompanied by increased viability and invasion and decreased apoptosis. 3-methyladenine 58-62 microtubule associated protein 1 light chain 3 beta Homo sapiens 141-145 27514525-7 2016 Further studies demonstrated that after pretreatment with 3-MA (3 mmol/L) for 48 h, siKDM4A-transfected cells showed a prominent decrease in LC3B-II/LC3B-I and Beclin 1, accompanied by increased viability and invasion and decreased apoptosis. 3-methyladenine 58-62 microtubule associated protein 1 light chain 3 beta Homo sapiens 149-153 27337297-6 2016 Additionally, astrocytes transfected with the GFP-MAP1LC3B plasmid also demonstrated cocaine-mediated upregulation of the green fluorescent MAP1LC3B puncta. Cocaine 85-92 microtubule associated protein 1 light chain 3 beta Homo sapiens 50-58 27337297-6 2016 Additionally, astrocytes transfected with the GFP-MAP1LC3B plasmid also demonstrated cocaine-mediated upregulation of the green fluorescent MAP1LC3B puncta. Cocaine 85-92 microtubule associated protein 1 light chain 3 beta Homo sapiens 140-148 27172402-0 2016 BAX-BAK1-independent LC3B lipidation by BH3 mimetics is unrelated to BH3 mimetic activity and has only minimal effects on autophagic flux. BH 3 40-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 21-25 27172402-3 2016 Here we confirm that small molecule BH3 mimetics can induce BAX- and BAK1-independent MAP1LC3B/LC3B lipidation, but this only occurred at concentrations far greater than required to induce apoptosis and dissociate canonical BH3 domain-containing proteins that bind more tightly than BECN1. BH 3 36-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 86-94 27172402-3 2016 Here we confirm that small molecule BH3 mimetics can induce BAX- and BAK1-independent MAP1LC3B/LC3B lipidation, but this only occurred at concentrations far greater than required to induce apoptosis and dissociate canonical BH3 domain-containing proteins that bind more tightly than BECN1. BH 3 36-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 90-94 27172402-4 2016 Because high concentrations of a less-active enantiomer of ABT-263 also induced BAX- and BAK1-independent LC3B lipidation, induction of this marker of autophagy appears to be an off-target effect. 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid 59-62 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 27250034-5 2016 In EAC cell lines, an increase in LC3B and p62 was observed with increasing concentrations of the autophagy inhibitor chloroquine, which indicates functional basal autophagy. Chloroquine 118-129 microtubule associated protein 1 light chain 3 beta Homo sapiens 34-38 27554119-10 2016 RNA interference of LC3B in these MnCl2-exposed cells demonstrated that MMP loss and apoptosis were enhanced. manganese chloride 34-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 20-24 27177023-4 2016 Cedrol-triggered autophagy was confirmed by transmission electron microscopy (TEM) analysis of the cells, as well as by western blot analysis of microtubule-associated protein light-chain 3 (LC3)B expression. cedrol 0-6 microtubule associated protein 1 light chain 3 beta Homo sapiens 145-196 27109915-5 2016 When treated with radiation alone, human esophageal carcinoma xenografts showed increased LC3B and p-LKB1 expression, which was decreased by the autophagy inhibitor chloroquine. Chloroquine 165-176 microtubule associated protein 1 light chain 3 beta Homo sapiens 90-94 27328819-5 2016 Here, we found that trehalose treatment increased the LC3-I to LC3-II conversion, acridine orange-stained vacuoles and GFP-LC3B (LC3B protein tagged with green fluorescent protein) puncta in the HaCaT human keratinocyte cell line, indicating autophagy induction. Trehalose 20-29 microtubule associated protein 1 light chain 3 beta Homo sapiens 123-127 27328819-5 2016 Here, we found that trehalose treatment increased the LC3-I to LC3-II conversion, acridine orange-stained vacuoles and GFP-LC3B (LC3B protein tagged with green fluorescent protein) puncta in the HaCaT human keratinocyte cell line, indicating autophagy induction. Trehalose 20-29 microtubule associated protein 1 light chain 3 beta Homo sapiens 129-133 27243769-6 2016 Moreover, we demonstrated that Stel B induced autophagy in A549 cells by use of various assays including monodansylcadaverine (MDC) staining, transmission electron microscopy (TEM), tandem mRFP-GFP-LC3 fluorescence microscopy, and western blot detection of the autophagy markers of LC3B, p62 and Atg5. stellettin B 31-37 microtubule associated protein 1 light chain 3 beta Homo sapiens 282-286 26635914-8 2016 However, the potent antioxidant, N-acetylcysteine (NAC) abrogated the effects of IH by inducing large CD66b(+)/LC3B(+) Gphi and increased both NADPH oxidase expression and phagocytosis. Acetylcysteine 33-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 111-115 27116987-21 2016 (2)The expression of LC3B of rapamycin + cisplatin group was higher than those of other 3 group cells(1.72+-0.08, 1.43+-0.04, 1.37+-0.11, and 1.11+-0.09; P<0.05). Sirolimus 29-38 microtubule associated protein 1 light chain 3 beta Homo sapiens 21-25 27116987-21 2016 (2)The expression of LC3B of rapamycin + cisplatin group was higher than those of other 3 group cells(1.72+-0.08, 1.43+-0.04, 1.37+-0.11, and 1.11+-0.09; P<0.05). Cisplatin 41-50 microtubule associated protein 1 light chain 3 beta Homo sapiens 21-25 26511816-8 2016 Combined with Beclin-1 and LC3B, Luminacin induced autophagic cell death in head and neck cancer cells. luminacin 33-42 microtubule associated protein 1 light chain 3 beta Homo sapiens 27-31 26764007-6 2016 We observe that the autophagy markers LC3b and lysosome-associated membrane protein 2A (LAMP2A) localize to a subset of TRIM5alpha cytoplasmic bodies, and inhibition of lysosomal degradation with bafilomycin A1 increases this association. bafilomycin 196-207 microtubule associated protein 1 light chain 3 beta Homo sapiens 38-42 26635914-8 2016 However, the potent antioxidant, N-acetylcysteine (NAC) abrogated the effects of IH by inducing large CD66b(+)/LC3B(+) Gphi and increased both NADPH oxidase expression and phagocytosis. Acetylcysteine 51-54 microtubule associated protein 1 light chain 3 beta Homo sapiens 111-115 26884987-0 2015 beta-asarone prevents Abeta25-35-induced inflammatory responses and autophagy in SH-SY5Y cells: down expression Beclin-1, LC3B and up expression Bcl-2. asarone 0-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 122-126 26468287-9 2015 The specificity for binding to LC3A/B is due to the interaction between Asp(1285) in FYCO1 and His(57) in LC3B. Histidine 95-98 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 26445356-3 2015 Cd-induced autophagy in human hepatoma (HepG2) cells has been demonstrated by green fluorescent protein (GFP)-LC3B plasmid DNA transfection, and LC3-II conversion by autophagy inhibitors. Cadmium 0-2 microtubule associated protein 1 light chain 3 beta Homo sapiens 110-114 26715839-6 2015 When exposed to cisplatin, lung adeno-carcinoma cells demonstrated increased levels of autophagy detected by MAP1A/1B LC3B and mammalian homologue of yeast Atg6 (Beclin-1) expression using Western blot analysis. Cisplatin 16-25 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-122 26715839-7 2015 Activation of cisplatin-induced autophagic flux was increased by using chloroquine (CQ), which can accumulate LC3B-II protein and increase punctate distribution of LC3B localization. Cisplatin 14-23 microtubule associated protein 1 light chain 3 beta Homo sapiens 110-114 26715839-7 2015 Activation of cisplatin-induced autophagic flux was increased by using chloroquine (CQ), which can accumulate LC3B-II protein and increase punctate distribution of LC3B localization. Cisplatin 14-23 microtubule associated protein 1 light chain 3 beta Homo sapiens 164-168 26715839-7 2015 Activation of cisplatin-induced autophagic flux was increased by using chloroquine (CQ), which can accumulate LC3B-II protein and increase punctate distribution of LC3B localization. Chloroquine 71-82 microtubule associated protein 1 light chain 3 beta Homo sapiens 110-114 26715839-7 2015 Activation of cisplatin-induced autophagic flux was increased by using chloroquine (CQ), which can accumulate LC3B-II protein and increase punctate distribution of LC3B localization. Chloroquine 71-82 microtubule associated protein 1 light chain 3 beta Homo sapiens 164-168 26715839-7 2015 Activation of cisplatin-induced autophagic flux was increased by using chloroquine (CQ), which can accumulate LC3B-II protein and increase punctate distribution of LC3B localization. Chloroquine 84-86 microtubule associated protein 1 light chain 3 beta Homo sapiens 110-114 26715839-7 2015 Activation of cisplatin-induced autophagic flux was increased by using chloroquine (CQ), which can accumulate LC3B-II protein and increase punctate distribution of LC3B localization. Chloroquine 84-86 microtubule associated protein 1 light chain 3 beta Homo sapiens 164-168 26715839-9 2015 Compared to cisplatin treatment alone, the combination of cisplatin and CQ decreased p-AMPK and increased p-mTOR protein expressions, in addition, the AMPK inhibitor Compound C plus cisplatin downregulated p-AMPK and upregulated p-mTOR as well as depressed LC3B cleavage. Cisplatin 58-67 microtubule associated protein 1 light chain 3 beta Homo sapiens 257-261 26715839-9 2015 Compared to cisplatin treatment alone, the combination of cisplatin and CQ decreased p-AMPK and increased p-mTOR protein expressions, in addition, the AMPK inhibitor Compound C plus cisplatin downregulated p-AMPK and upregulated p-mTOR as well as depressed LC3B cleavage. Chloroquine 72-74 microtubule associated protein 1 light chain 3 beta Homo sapiens 257-261 26715839-9 2015 Compared to cisplatin treatment alone, the combination of cisplatin and CQ decreased p-AMPK and increased p-mTOR protein expressions, in addition, the AMPK inhibitor Compound C plus cisplatin downregulated p-AMPK and upregulated p-mTOR as well as depressed LC3B cleavage. Cisplatin 58-67 microtubule associated protein 1 light chain 3 beta Homo sapiens 257-261 26468287-9 2015 The specificity for binding to LC3A/B is due to the interaction between Asp(1285) in FYCO1 and His(57) in LC3B. Aspartic Acid 72-75 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 26576535-8 2015 Moreover, AIS patients with higher levels of Beclin1 and LC3B in CSF had significantly higher improvement in NIHSS. nihss 109-114 microtubule associated protein 1 light chain 3 beta Homo sapiens 57-61 26884987-8 2015 Meanwhile, beta-asarone could significantly reduce Beclin-1, LC3B and increase anti-apoptotic protein Bcl-2 level. asarone 11-23 microtubule associated protein 1 light chain 3 beta Homo sapiens 61-65 26378019-7 2015 Importantly, oral delivery of C-PAC significantly inhibited OE19 tumor xenograft growth via modulation of AKT/mTOR/MAPK signaling and induction of the autophagic form of LC3B supporting in vivo efficacy against EAC for the first time. c-pac 30-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 170-174 26005215-9 2015 Real-time PCR revealed that cisplatin upregulated multiple autophagy-related genes, including AMBRA1, ATG3, ATG4C, ATG4D, ATG5, ATG7, ATG13, ATG14, ATG16L2, Beclin1, DRAM1, GABARAP, GABARAPL1, GABARAPL2, HDAC6, IRGM, MAP1LC3B and ULK1, indicating that cisplatin induced autophagy through a multiple mechanism involved manner. Cisplatin 28-37 microtubule associated protein 1 light chain 3 beta Homo sapiens 217-225 26204837-6 2015 In addition, fluoxetine up-regulated SQSTM1 and LC3B protein expression as detected by western blotting and immunofluorescence. Fluoxetine 13-23 microtubule associated protein 1 light chain 3 beta Homo sapiens 48-52 26204837-7 2015 The autophagy inhibitor, 3-methyladenine (3-MA), significantly attenuated fluoxetine-mediated effects on ASC proliferation and SQSTM1/LC3B expression. 3-methyladenine 25-40 microtubule associated protein 1 light chain 3 beta Homo sapiens 134-138 26204837-7 2015 The autophagy inhibitor, 3-methyladenine (3-MA), significantly attenuated fluoxetine-mediated effects on ASC proliferation and SQSTM1/LC3B expression. 3-methyladenine 42-46 microtubule associated protein 1 light chain 3 beta Homo sapiens 134-138 26204837-7 2015 The autophagy inhibitor, 3-methyladenine (3-MA), significantly attenuated fluoxetine-mediated effects on ASC proliferation and SQSTM1/LC3B expression. Fluoxetine 74-84 microtubule associated protein 1 light chain 3 beta Homo sapiens 134-138 25997470-5 2015 Quercetin treatment dose-dependently decreased the p62 protein expression and increased GFP-LC3B. Quercetin 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 92-96 26345371-9 2015 Short-term treatment of 2DG induced autophagic flux, which increased microtubule associated protein 1 light chain 3B (LC3B) conversion rates and reduced p62 levels. Deoxyglucose 24-27 microtubule associated protein 1 light chain 3 beta Homo sapiens 118-122 26158232-6 2015 By using dual-immunofluorescence staining, we demonstrated that increased LC3B was located mainly in the cytokeratin 19-labeled ductular reaction (DR) in human cirrhotic livers and in an experimental cirrhosis induced by 2-acetylaminofluorene (AAF) with carbon tetrachloride (CCl4), indicating a conserved response to chronic liver damage. 2-Acetylaminofluorene 221-242 microtubule associated protein 1 light chain 3 beta Homo sapiens 74-78 26158232-6 2015 By using dual-immunofluorescence staining, we demonstrated that increased LC3B was located mainly in the cytokeratin 19-labeled ductular reaction (DR) in human cirrhotic livers and in an experimental cirrhosis induced by 2-acetylaminofluorene (AAF) with carbon tetrachloride (CCl4), indicating a conserved response to chronic liver damage. 2-Acetylaminofluorene 244-247 microtubule associated protein 1 light chain 3 beta Homo sapiens 74-78 26158232-6 2015 By using dual-immunofluorescence staining, we demonstrated that increased LC3B was located mainly in the cytokeratin 19-labeled ductular reaction (DR) in human cirrhotic livers and in an experimental cirrhosis induced by 2-acetylaminofluorene (AAF) with carbon tetrachloride (CCl4), indicating a conserved response to chronic liver damage. Carbon Tetrachloride 254-274 microtubule associated protein 1 light chain 3 beta Homo sapiens 74-78 26171034-11 2015 The expression of autophagy molecular markers, Beclin-1 and LC3-B, was increased following DHA treatment, while no significant alteration was detected in the expression of apoptotic marker Caspase-3. artenimol 91-94 microtubule associated protein 1 light chain 3 beta Homo sapiens 60-65 26150155-3 2015 Here we show the feasibility of specifically and reliably assessing the autophagy markers LC3B and p62 (SQSTM1) in formalin fixed and paraffin embedded human tissue by immunohistochemistry. Formaldehyde 115-123 microtubule associated protein 1 light chain 3 beta Homo sapiens 90-94 26150155-6 2015 LC3B and p62 antibodies were validated on formalin fixed and paraffin embedded cell pellets of treated and control cells and finally applied on a tissue microarray with 80 human malignant and non-neoplastic lung and stomach formalin fixed and paraffin embedded tissue samples. Formaldehyde 42-50 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 26150155-11 2015 In summary, we present a specific and reliable IHC staining of LC3B and p62 on formalin fixed and paraffin embedded human tissue. Formaldehyde 79-87 microtubule associated protein 1 light chain 3 beta Homo sapiens 63-67 26150155-11 2015 In summary, we present a specific and reliable IHC staining of LC3B and p62 on formalin fixed and paraffin embedded human tissue. Paraffin 98-106 microtubule associated protein 1 light chain 3 beta Homo sapiens 63-67 25925964-11 2015 Furthermore, EGCG and L-theanine inhibited TNF-alpha-induced apoptosis-related gene expression (e.g., CASP9), and caspase activity while inhibiting TNFalpha-induced VCAM1, LC3A and LC3B protein expression. epigallocatechin gallate 13-17 microtubule associated protein 1 light chain 3 beta Homo sapiens 181-185 25925964-11 2015 Furthermore, EGCG and L-theanine inhibited TNF-alpha-induced apoptosis-related gene expression (e.g., CASP9), and caspase activity while inhibiting TNFalpha-induced VCAM1, LC3A and LC3B protein expression. theanine 22-32 microtubule associated protein 1 light chain 3 beta Homo sapiens 181-185 25806943-7 2015 Isorhamnetin treatment reduced virus-induced ROS generation and blocked cytoplasmic lysosome acidification and the lipidation of microtubule associated protein1 light chain 3-B (LC3B). 3-methylquercetin 0-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 178-182 25633408-8 2015 LPA further increases the expression of LC3B II in the presence of H2O2. lysophosphatidic acid 0-3 microtubule associated protein 1 light chain 3 beta Homo sapiens 40-44 25619389-10 2015 Moreover, PL markedly increased the expression of the apoptotic proteins (Bax, Bcl-2 and caspase-3) and autophagic proteins (Beclin-1 and LC3B), and phosphorylation of p38 and JNK in BMMNCs from the patients with myeloid leukemias, whereas pretreatment with the specific p38 inhibitor SB203580 or the specific JNK inhibitor SP600125 partially reversed PL-induced ROS production, apoptotic/autophagic signaling activation and cytotoxicity. piperlonguminine 10-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 138-142 25633408-7 2015 Additionally, H2O2 increases levels of type II of light chain 3B (LC3B II), an autophagy marker, and H2O2-induced autophagy thus protected BMSCs from apoptosis. Hydrogen Peroxide 14-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 66-70 25633408-8 2015 LPA further increases the expression of LC3B II in the presence of H2O2. Hydrogen Peroxide 67-71 microtubule associated protein 1 light chain 3 beta Homo sapiens 40-44 25227893-8 2015 DM induced a characteristic morphological change with the appearance of numerous LC3B-containing acidic vacuoles and an increase in LC3BII levels. dorsomorphin 0-2 microtubule associated protein 1 light chain 3 beta Homo sapiens 81-85 25700560-9 2015 We next determined that autophagy and mitophagy were increased by ammonia by measuring autophagic proteolysis of long-lived proteins, increase of autophagy markers MAP1LC3B, GABARAP, and GABARAPL2, mitophagy markers BNIP3 and the PINK1-PARK2 system as well as mitochondrial morphology and dynamics. Ammonia 66-73 microtubule associated protein 1 light chain 3 beta Homo sapiens 164-172 25607473-5 2015 LC3B inhibition was achieved by small-interfering RNA (siRNA) targeting LC3B or by treatment with 3-methyladenine (3-MA). 3-methyladenine 98-113 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 25607473-5 2015 LC3B inhibition was achieved by small-interfering RNA (siRNA) targeting LC3B or by treatment with 3-methyladenine (3-MA). 3-methyladenine 115-119 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 25607473-9 2015 LC3B siRNA and 3-MA treatment each attenuated hypoxia-induced LC3B expression, along with migration and invasion, and this was associated with a decrease in RhoA expression and disorganization of the actin cytoskeleton. 3-methyladenine 15-19 microtubule associated protein 1 light chain 3 beta Homo sapiens 62-66 25779723-9 2015 Surprisingly, the radiosensitizing effect of 3-methyladenine was also observed in ATG7 and LC3b deficient cells and was associated with attenuated gamma-H2AX formation and DNA damage repair. 3-methyladenine 45-60 microtubule associated protein 1 light chain 3 beta Homo sapiens 91-95 25490467-6 2015 RESULTS: We demonstrate that the C-terminal part of Vif interacts directly with LC3B, independently of the presence of APOBEC3G.Vif binds to pro-LC3 and autophagy-related protein 4-cleaved LC3 forms, and glycine 120, the amino acid conjugated to phosphatidylethanolamine on autophagosomes, is required. pro-lc3 141-148 microtubule associated protein 1 light chain 3 beta Homo sapiens 80-84 25490467-6 2015 RESULTS: We demonstrate that the C-terminal part of Vif interacts directly with LC3B, independently of the presence of APOBEC3G.Vif binds to pro-LC3 and autophagy-related protein 4-cleaved LC3 forms, and glycine 120, the amino acid conjugated to phosphatidylethanolamine on autophagosomes, is required. Glycine 204-211 microtubule associated protein 1 light chain 3 beta Homo sapiens 80-84 25490467-6 2015 RESULTS: We demonstrate that the C-terminal part of Vif interacts directly with LC3B, independently of the presence of APOBEC3G.Vif binds to pro-LC3 and autophagy-related protein 4-cleaved LC3 forms, and glycine 120, the amino acid conjugated to phosphatidylethanolamine on autophagosomes, is required. phosphatidylethanolamine 246-270 microtubule associated protein 1 light chain 3 beta Homo sapiens 80-84 25749095-2 2015 Thus, RA enhanced autophagy in TLR9- and CD180-stimulated peripheral blood B cells, as revealed by increased levels of the autophagosomal marker LC3B-II, enhanced colocalization between LC3B and the lysosomal marker Lyso-ID, by a larger percentage of cells with more than 5 characteristic LC3B puncta, and by the concomitant reduction in the level of SQSTM1/p62. Tretinoin 6-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 145-149 25749095-2 2015 Thus, RA enhanced autophagy in TLR9- and CD180-stimulated peripheral blood B cells, as revealed by increased levels of the autophagosomal marker LC3B-II, enhanced colocalization between LC3B and the lysosomal marker Lyso-ID, by a larger percentage of cells with more than 5 characteristic LC3B puncta, and by the concomitant reduction in the level of SQSTM1/p62. Tretinoin 6-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 186-190 25749095-2 2015 Thus, RA enhanced autophagy in TLR9- and CD180-stimulated peripheral blood B cells, as revealed by increased levels of the autophagosomal marker LC3B-II, enhanced colocalization between LC3B and the lysosomal marker Lyso-ID, by a larger percentage of cells with more than 5 characteristic LC3B puncta, and by the concomitant reduction in the level of SQSTM1/p62. Tretinoin 6-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 186-190 25714112-3 2015 PtdIns3P associates with growing phagophores, which recruit components of the autophagic machinery, including the lipidated form of MAP1LC3B/LC3 (microtubule-associated protein 1 light chain 3 beta). phosphatidylinositol 3-phosphate 0-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 132-140 25474306-6 2014 Under gentamycin and m.1494C>T stress together, more dysfunctional mitochondria were forced to fuse and exhibited mitophagy via up-regulated LC3-B, as a compensatory protective response to try to optimize mitochondrial function, rather than undergo apoptosis. Gentamicins 6-16 microtubule associated protein 1 light chain 3 beta Homo sapiens 144-149 25714112-3 2015 PtdIns3P associates with growing phagophores, which recruit components of the autophagic machinery, including the lipidated form of MAP1LC3B/LC3 (microtubule-associated protein 1 light chain 3 beta). phosphatidylinositol 3-phosphate 0-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 146-197 25714112-4 2015 Depletion of BECN1, PIK3C3 or some of their interactors suppresses the formation of MAP1LC3B(+) phagophores or autophagosomes elicited by most physiological stimuli, including saturated fatty acids. Fatty Acids 176-197 microtubule associated protein 1 light chain 3 beta Homo sapiens 84-92 25714112-5 2015 We observed that cis-unsaturated fatty acids stimulate the generation of cytosolic puncta containing lipidated MAP1LC3B as well as the autophagic turnover of long-lived proteins in the absence of PtdIns3P accumulation. cis-unsaturated fatty acids 17-44 microtubule associated protein 1 light chain 3 beta Homo sapiens 111-119 25714112-8 2015 Importantly, MAP1LC3B(+) puncta elicited by cis-unsaturated fatty acids colocalize with Golgi apparatus markers. cis-unsaturated fatty acids 44-71 microtubule associated protein 1 light chain 3 beta Homo sapiens 13-21 25893854-2 2015 Here, we screened the North China Pharmaceutical Group Corporation"s pure compound library of microbial origin using GFP-LC3B-SKOV3 cells and identified elaiophylin as a novel autophagy inhibitor. elaiophylin 153-164 microtubule associated protein 1 light chain 3 beta Homo sapiens 121-125 25375091-5 2014 The combination of low-dose NVP-AUY922 with rapamycin had comparable effects on reducing KIT expression, increasing MAP1LC3B puncta and tumor necrosis, and inhibiting tumor growth as high-dose NVP-AUY922 did in GIST430 xenograft model. Sirolimus 44-53 microtubule associated protein 1 light chain 3 beta Homo sapiens 116-124 25486109-6 2014 Chromomycin A2 treated cells depicted several cellular fragments resembling autophagosomes and increased expression of proteins LC3-A and LC3-B. chromomycin A2 0-14 microtubule associated protein 1 light chain 3 beta Homo sapiens 138-143 25236743-5 2014 Early DNFB induction of ATF4 positively modulates autophagy-related genes MAP1LC3B and ATG3 and stabilizes the transcription factor Nrf2, causing a strong induction of the HMOX1-detoxifying gene. Dinitrofluorobenzene 6-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 74-82 25285629-5 2014 In both cancer cell lines, GDC-0980 simultaneously activated apoptosis and autophagy, the latter was detected by p62 degradation, Beclin-1 upregulation and light chain 3B (LC3B) conversion from a cytosolic (LC3B-I) to a membrane-bound (LC3B-II) form. 1-(4-((2-(2-aminopyrimidin-5-yl)-7-methyl-4-morpholinothieno(3,2-d)pyrimidin-6-yl)methyl)piperazin-1-yl)-2-hydroxypropan-1-one 27-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 156-170 25517751-2 2014 Here we establish, using shRNAs, a dominant-negative mutant, and a pharmacologic inhibitor, mefenamic acid (MFA), that the Transient Receptor Potential Melastatin 3 (TRPM3) channel promotes the growth of clear cell renal cell carcinoma (ccRCC) and stimulates MAP1LC3A (LC3A) and MAP1LC3B (LC3B) autophagy. Mefenamic Acid 92-106 microtubule associated protein 1 light chain 3 beta Homo sapiens 279-287 25517751-2 2014 Here we establish, using shRNAs, a dominant-negative mutant, and a pharmacologic inhibitor, mefenamic acid (MFA), that the Transient Receptor Potential Melastatin 3 (TRPM3) channel promotes the growth of clear cell renal cell carcinoma (ccRCC) and stimulates MAP1LC3A (LC3A) and MAP1LC3B (LC3B) autophagy. Mefenamic Acid 92-106 microtubule associated protein 1 light chain 3 beta Homo sapiens 283-287 25517751-2 2014 Here we establish, using shRNAs, a dominant-negative mutant, and a pharmacologic inhibitor, mefenamic acid (MFA), that the Transient Receptor Potential Melastatin 3 (TRPM3) channel promotes the growth of clear cell renal cell carcinoma (ccRCC) and stimulates MAP1LC3A (LC3A) and MAP1LC3B (LC3B) autophagy. Mefenamic Acid 108-111 microtubule associated protein 1 light chain 3 beta Homo sapiens 279-287 25517751-2 2014 Here we establish, using shRNAs, a dominant-negative mutant, and a pharmacologic inhibitor, mefenamic acid (MFA), that the Transient Receptor Potential Melastatin 3 (TRPM3) channel promotes the growth of clear cell renal cell carcinoma (ccRCC) and stimulates MAP1LC3A (LC3A) and MAP1LC3B (LC3B) autophagy. Mefenamic Acid 108-111 microtubule associated protein 1 light chain 3 beta Homo sapiens 283-287 25517751-4 2014 In addition, TRPM3 Ca(2+) and Zn(2+) fluxes inhibit miR-214, which directly targets LC3A and LC3B. Zinc 30-32 microtubule associated protein 1 light chain 3 beta Homo sapiens 93-97 25285629-5 2014 In both cancer cell lines, GDC-0980 simultaneously activated apoptosis and autophagy, the latter was detected by p62 degradation, Beclin-1 upregulation and light chain 3B (LC3B) conversion from a cytosolic (LC3B-I) to a membrane-bound (LC3B-II) form. 1-(4-((2-(2-aminopyrimidin-5-yl)-7-methyl-4-morpholinothieno(3,2-d)pyrimidin-6-yl)methyl)piperazin-1-yl)-2-hydroxypropan-1-one 27-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 172-176 25285629-5 2014 In both cancer cell lines, GDC-0980 simultaneously activated apoptosis and autophagy, the latter was detected by p62 degradation, Beclin-1 upregulation and light chain 3B (LC3B) conversion from a cytosolic (LC3B-I) to a membrane-bound (LC3B-II) form. 1-(4-((2-(2-aminopyrimidin-5-yl)-7-methyl-4-morpholinothieno(3,2-d)pyrimidin-6-yl)methyl)piperazin-1-yl)-2-hydroxypropan-1-one 27-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 207-211 25285629-5 2014 In both cancer cell lines, GDC-0980 simultaneously activated apoptosis and autophagy, the latter was detected by p62 degradation, Beclin-1 upregulation and light chain 3B (LC3B) conversion from a cytosolic (LC3B-I) to a membrane-bound (LC3B-II) form. 1-(4-((2-(2-aminopyrimidin-5-yl)-7-methyl-4-morpholinothieno(3,2-d)pyrimidin-6-yl)methyl)piperazin-1-yl)-2-hydroxypropan-1-one 27-35 microtubule associated protein 1 light chain 3 beta Homo sapiens 207-211 25285629-7 2014 Furthermore, knockdown of LC3B or Beclin-1 through siRNA increased GDC-0980-induced anti-pancreatic cancer cell activity. 1-(4-((2-(2-aminopyrimidin-5-yl)-7-methyl-4-morpholinothieno(3,2-d)pyrimidin-6-yl)methyl)piperazin-1-yl)-2-hydroxypropan-1-one 67-75 microtubule associated protein 1 light chain 3 beta Homo sapiens 26-30 25271424-8 2014 Pinocembrin-treatment could also reduce the number of TUNEL-positive and Caspase-3-positive neurons, and upregulate the expression of LC3B and Beclin1 in penumbra area. pinocembrin 0-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 134-138 25128814-4 2014 Use of the autophagy inhibitors chloroquine and bafilomycin A1, or RNA interference of ATG7, suggested that these increases in LC3B and SQSTM1 levels were in part attributed to altered autophagic flux. Chloroquine 32-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 127-131 24907655-7 2014 The suppression of autophagy by pharmacological inhibitors or LC3B and beclin-1 genetic knockdown rescued REH cells from idarubicin-mediated oxidative stress, mitochondrial depolarization, caspase activation and apoptotic DNA fragmentation. Idarubicin 121-131 microtubule associated protein 1 light chain 3 beta Homo sapiens 62-66 25414767-5 2014 Interestingly, gene silencing of LC3B, an autophagy marker, significantly enhanced gAcrp-induced apoptosis in both HepG2 and MCF-7 cell lines, whereas induction of autophagy by rapamycin, an mTOR inhibitor, significantly prevented gAcrp-induced apoptosis in hepatoma cells HepG2. gacrp 83-88 microtubule associated protein 1 light chain 3 beta Homo sapiens 33-37 25414767-5 2014 Interestingly, gene silencing of LC3B, an autophagy marker, significantly enhanced gAcrp-induced apoptosis in both HepG2 and MCF-7 cell lines, whereas induction of autophagy by rapamycin, an mTOR inhibitor, significantly prevented gAcrp-induced apoptosis in hepatoma cells HepG2. gacrp 231-236 microtubule associated protein 1 light chain 3 beta Homo sapiens 33-37 24907655-3 2014 Idarubicin increased punctuation/aggregation of microtubule-associated light chain 3B (LC3B), enhanced the conversion of LC3B-I to autophagosome-associated LC3B-II in the presence of proteolysis inhibitors, and promoted the degradation of the selective autophagic target p62, thus indicating the increase in autophagic flux. Idarubicin 0-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 87-91 24905917-7 2014 Meanwhile, the MAPK11/12/13/14 inhibitor SB203580 downregulated autophagy by inhibiting the transcription of the autophagy genes MAP1LC3B, BAG3, and HSPA1A, and promoted HYF127c/Cu-induced cell death. SB 203580 41-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 129-137 24907655-3 2014 Idarubicin increased punctuation/aggregation of microtubule-associated light chain 3B (LC3B), enhanced the conversion of LC3B-I to autophagosome-associated LC3B-II in the presence of proteolysis inhibitors, and promoted the degradation of the selective autophagic target p62, thus indicating the increase in autophagic flux. Idarubicin 0-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 121-125 24907655-3 2014 Idarubicin increased punctuation/aggregation of microtubule-associated light chain 3B (LC3B), enhanced the conversion of LC3B-I to autophagosome-associated LC3B-II in the presence of proteolysis inhibitors, and promoted the degradation of the selective autophagic target p62, thus indicating the increase in autophagic flux. Idarubicin 0-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 121-125 24819607-6 2014 Upon ceramide treatment, phosphorylated EMD binds MAP1LC3B leading to an increase of autophagosome formation. Ceramides 5-13 microtubule associated protein 1 light chain 3 beta Homo sapiens 50-58 24963720-8 2014 Zoledronic acid treatment upregulated reactive oxygen species as well as the autophagy marker protein LC-3B. Zoledronic Acid 0-15 microtubule associated protein 1 light chain 3 beta Homo sapiens 102-107 24418614-8 2014 Critically, cyclic adenosine monophosphate (cAMP) treatment reacidified lysosomal pH in mutant PS1-fAD; cAMP also increased the availability of active cathepsin D and lowered the LC3B-II/-I ratio. Cyclic AMP 44-48 microtubule associated protein 1 light chain 3 beta Homo sapiens 179-183 24492484-10 2014 LC3B lipidation increased with acetaldehyde treatment and increased further with the addition of cyanamide. Acetaldehyde 31-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 24492484-10 2014 LC3B lipidation increased with acetaldehyde treatment and increased further with the addition of cyanamide. Cyanamide 97-106 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 24492484-6 2014 Correspondingly, ethanol-treated C2C12 myotubes stably expressing GFP-LC3B showed increased autophagy flux as measured by accumulation of GFP-LC3B vesicles with confocal microscopy. Ethanol 17-24 microtubule associated protein 1 light chain 3 beta Homo sapiens 70-74 24492484-6 2014 Correspondingly, ethanol-treated C2C12 myotubes stably expressing GFP-LC3B showed increased autophagy flux as measured by accumulation of GFP-LC3B vesicles with confocal microscopy. Ethanol 17-24 microtubule associated protein 1 light chain 3 beta Homo sapiens 142-146 24492484-7 2014 The ethanol-induced increase in LC3B lipidation was reversed upon knockdown of Atg7, a critical autophagy gene and was associated with reversal of the ethanol-induced decrease in myotube diameter. Ethanol 4-11 microtubule associated protein 1 light chain 3 beta Homo sapiens 32-36 24492484-7 2014 The ethanol-induced increase in LC3B lipidation was reversed upon knockdown of Atg7, a critical autophagy gene and was associated with reversal of the ethanol-induced decrease in myotube diameter. Ethanol 151-158 microtubule associated protein 1 light chain 3 beta Homo sapiens 32-36 24418614-8 2014 Critically, cyclic adenosine monophosphate (cAMP) treatment reacidified lysosomal pH in mutant PS1-fAD; cAMP also increased the availability of active cathepsin D and lowered the LC3B-II/-I ratio. Adenosine 19-28 microtubule associated protein 1 light chain 3 beta Homo sapiens 179-183 24418614-8 2014 Critically, cyclic adenosine monophosphate (cAMP) treatment reacidified lysosomal pH in mutant PS1-fAD; cAMP also increased the availability of active cathepsin D and lowered the LC3B-II/-I ratio. Cyclic AMP 104-108 microtubule associated protein 1 light chain 3 beta Homo sapiens 179-183 24264887-6 2014 Using an EGFP-LC3B transfection and confocal laser microscopy approach, we found RSV-induced EGFP-LC3 puncta co-localized with ER-tracker red dye, implicating the involvement of LC3II in ER expansion. Resveratrol 81-84 microtubule associated protein 1 light chain 3 beta Homo sapiens 14-18 24628795-9 2014 8-Cl-Ado-mediated induction of autophagy was evident by increased aggregates of microtubule-associated protein 1 light chain 3B (LC3B) which was associated with its conversion to its lipidated form, LC3B-II, p62 degradative flux, and increased formation of acidic vesicular organelles. 8-chloroadenosine 0-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 129-133 24628795-9 2014 8-Cl-Ado-mediated induction of autophagy was evident by increased aggregates of microtubule-associated protein 1 light chain 3B (LC3B) which was associated with its conversion to its lipidated form, LC3B-II, p62 degradative flux, and increased formation of acidic vesicular organelles. 8-chloroadenosine 0-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 199-203 24209807-11 2014 Expression of Beclin-1, LC3A, and LC3B increased in HepG2 cells in response to TG, similar to the response seen in vivo. Thapsigargin 79-81 microtubule associated protein 1 light chain 3 beta Homo sapiens 34-38 24384561-3 2014 In this study, we identified several ATG genes, including the genes ULK1, MAP1LC3B, GABARAPL1, ATG13, WIPI1, and WDR45/WIPI4, with elevated mRNA levels in thapsigargin-, C2-ceramide-, and rapamycin-treated as well as amino acid-depleted HeLa cells except for MAP1LC3B mRNA in rapamycin-treated HeLa cells. Thapsigargin 155-167 microtubule associated protein 1 light chain 3 beta Homo sapiens 74-82 24384561-3 2014 In this study, we identified several ATG genes, including the genes ULK1, MAP1LC3B, GABARAPL1, ATG13, WIPI1, and WDR45/WIPI4, with elevated mRNA levels in thapsigargin-, C2-ceramide-, and rapamycin-treated as well as amino acid-depleted HeLa cells except for MAP1LC3B mRNA in rapamycin-treated HeLa cells. Thapsigargin 155-167 microtubule associated protein 1 light chain 3 beta Homo sapiens 259-267 24384561-3 2014 In this study, we identified several ATG genes, including the genes ULK1, MAP1LC3B, GABARAPL1, ATG13, WIPI1, and WDR45/WIPI4, with elevated mRNA levels in thapsigargin-, C2-ceramide-, and rapamycin-treated as well as amino acid-depleted HeLa cells except for MAP1LC3B mRNA in rapamycin-treated HeLa cells. N-acetylsphingosine 170-181 microtubule associated protein 1 light chain 3 beta Homo sapiens 74-82 24384561-5 2014 The increase in WIPI1 and MAP1LC3B mRNA was induced prior to the accumulation of the autophagy marker protein MAP1LC3 in the thapsigargin- and C2-ceramide-treated A549 cells. Thapsigargin 125-137 microtubule associated protein 1 light chain 3 beta Homo sapiens 26-34 24384561-5 2014 The increase in WIPI1 and MAP1LC3B mRNA was induced prior to the accumulation of the autophagy marker protein MAP1LC3 in the thapsigargin- and C2-ceramide-treated A549 cells. N-acetylsphingosine 143-154 microtubule associated protein 1 light chain 3 beta Homo sapiens 26-34 24416349-3 2014 RAD001 and MK-2206 synergistically induced light chain 3B (LC3B) and beclin-1 expression, two important autophagy indicators. MK 2206 11-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 43-57 23847175-4 2014 LC3B showed an increase with CQ treatment although this was different to LysoTracker signals in terms of dose and time. Chloroquine 29-31 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 23847175-5 2014 Rapamycin, low serum (<1%) RPMI, and nutrient starvation induction of autophagy also induced an increase in LysoTracker and LC3B signals. Sirolimus 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 127-131 23847175-5 2014 Rapamycin, low serum (<1%) RPMI, and nutrient starvation induction of autophagy also induced an increase in LysoTracker and LC3B signals. rpmi 30-34 microtubule associated protein 1 light chain 3 beta Homo sapiens 127-131 23847175-6 2014 CQ also induced apoptosis in cell lines, which was blocked by pan-caspase inhibitor z-VAD resulting in a reduction in cells undergoing apoptosis and a subsequent upregulation of autophagic markers LC3B and lysosomal dye signals. Chloroquine 0-2 microtubule associated protein 1 light chain 3 beta Homo sapiens 197-201 23847175-6 2014 CQ also induced apoptosis in cell lines, which was blocked by pan-caspase inhibitor z-VAD resulting in a reduction in cells undergoing apoptosis and a subsequent upregulation of autophagic markers LC3B and lysosomal dye signals. z-vad 84-89 microtubule associated protein 1 light chain 3 beta Homo sapiens 197-201 24416349-3 2014 RAD001 and MK-2206 synergistically induced light chain 3B (LC3B) and beclin-1 expression, two important autophagy indicators. MK 2206 11-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 59-63 24349516-7 2013 Furthermore, there were differential changes in levels of beclin-1, DRAM, and LC3B-II in response to changes in oxygen and glucose levels. Oxygen 112-118 microtubule associated protein 1 light chain 3 beta Homo sapiens 78-82 25551567-4 2014 Olanzapine-mediated oxidative stress also induced mitochondrial depolarization and damage, and the autophagic clearance of dysfunctional mitochondria was confirmed by electron microscopy, colocalization of autophagosome-associated MAP1LC3B (LC3B henceforth) and mitochondria, and mitochondrial association with the autophagic cargo receptor SQSTM1/p62. Olanzapine 0-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 235-239 25551567-5 2014 While olanzapine-triggered mitochondrial damage was not overtly toxic to SH-SY5Y cells, their death was readily initiated upon the inhibition of autophagy with pharmacological inhibitors, RNA interference knockdown of BECN1 and LC3B, or biological free radical nitric oxide. Olanzapine 6-16 microtubule associated protein 1 light chain 3 beta Homo sapiens 228-232 25551567-6 2014 The treatment of mice with olanzapine for 14 d increased the brain levels of autophagosome-associated LC3B-II and mRNA encoding Atg4b, Atg5, Atg7, Atg12, Gabarap, and Becn1. Olanzapine 27-37 microtubule associated protein 1 light chain 3 beta Homo sapiens 102-106 25551567-4 2014 Olanzapine-mediated oxidative stress also induced mitochondrial depolarization and damage, and the autophagic clearance of dysfunctional mitochondria was confirmed by electron microscopy, colocalization of autophagosome-associated MAP1LC3B (LC3B henceforth) and mitochondria, and mitochondrial association with the autophagic cargo receptor SQSTM1/p62. Olanzapine 0-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 231-239 24358342-4 2013 Inhibition of autophagy by 3-methyladenine (3-MA), or by RNA interference (RNAi) of light chain 3B (LC3B), enhanced salinomycin-induced cytotoxicity and apoptosis. salinomycin 116-127 microtubule associated protein 1 light chain 3 beta Homo sapiens 84-98 24358342-4 2013 Inhibition of autophagy by 3-methyladenine (3-MA), or by RNA interference (RNAi) of light chain 3B (LC3B), enhanced salinomycin-induced cytotoxicity and apoptosis. salinomycin 116-127 microtubule associated protein 1 light chain 3 beta Homo sapiens 100-104 24349516-7 2013 Furthermore, there were differential changes in levels of beclin-1, DRAM, and LC3B-II in response to changes in oxygen and glucose levels. Glucose 123-130 microtubule associated protein 1 light chain 3 beta Homo sapiens 78-82 23815914-6 2013 NAC or Tiron, ROS scavenger, could decrease the FTY720 induced apoptosis and the expression of LC3B-II was reduced in combination of FTY720 with NAC or Tiron as compared with treatment with FTY720 only. 1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt 7-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 95-99 24085292-6 2013 Treatment with lysosomal inhibitors (ammonium chloride or chloroquine) or inhibitors of cathepsins (Z-FF-FMK or Z-FA-FMK) or knock-down of LC3B expression by siRNAs suppressed PI-induced IkappaBalpha degradation. Chloroquine 58-69 microtubule associated protein 1 light chain 3 beta Homo sapiens 139-143 24085292-6 2013 Treatment with lysosomal inhibitors (ammonium chloride or chloroquine) or inhibitors of cathepsins (Z-FF-FMK or Z-FA-FMK) or knock-down of LC3B expression by siRNAs suppressed PI-induced IkappaBalpha degradation. MDL 201053 112-120 microtubule associated protein 1 light chain 3 beta Homo sapiens 139-143 23942054-6 2013 The morphological effects and LC3B activation in presenilin-1 knockdown cells could be prevented by using the phosphoinositide 3-kinase (PI3K) inhibitor wortmannin or by calcium chelation. Wortmannin 153-163 microtubule associated protein 1 light chain 3 beta Homo sapiens 30-34 23942054-6 2013 The morphological effects and LC3B activation in presenilin-1 knockdown cells could be prevented by using the phosphoinositide 3-kinase (PI3K) inhibitor wortmannin or by calcium chelation. Calcium 170-177 microtubule associated protein 1 light chain 3 beta Homo sapiens 30-34 23688633-4 2013 Here, we demonstrated that gAcrp significantly restores ethanol-induced suppression of autophagy-related genes, including Beclin-1 and microtubule-associated protein light chain (LC3B) both in primary rat hepatocytes and human hepatoma cell line (HepG2). gacrp 27-32 microtubule associated protein 1 light chain 3 beta Homo sapiens 179-183 23688633-4 2013 Here, we demonstrated that gAcrp significantly restores ethanol-induced suppression of autophagy-related genes, including Beclin-1 and microtubule-associated protein light chain (LC3B) both in primary rat hepatocytes and human hepatoma cell line (HepG2). Ethanol 56-63 microtubule associated protein 1 light chain 3 beta Homo sapiens 179-183 23688633-6 2013 Furthermore, inhibition of gAcrp-induced autophagic process by knock-down of LC3B prevented protection from ethanol-induced apoptosis. Ethanol 108-115 microtubule associated protein 1 light chain 3 beta Homo sapiens 77-81 24040206-5 2013 The carboxyl-terminal five amino acids, particularly Lysine(122) and Leucine(123) of human LC3B play a major role in the faster migration of unprocessed LC3B, rendering it indistinguishable from LC3B-II in Wb assays. Leucine 69-76 microtubule associated protein 1 light chain 3 beta Homo sapiens 153-157 23792164-4 2013 Upregulated ATF4 results in the transcriptional initiation of the apoptosis-related chop gene and autophagy-related map1lc3b gene, through which selenite links ER stress to apoptosis and autophagy during leukemia treatment. Selenious Acid 145-153 microtubule associated protein 1 light chain 3 beta Homo sapiens 116-124 24349176-5 2013 Up-regulation of autophagy by rapamycin or GFP-LC3B-transfection alleviated celecoxib-induced cytotoxicity in UC cells. Celecoxib 76-85 microtubule associated protein 1 light chain 3 beta Homo sapiens 47-51 23750556-5 2013 Moreover, resveratrol treatment increased the expression of autophagy-regulated genes such as gamma-aminobutyric acid A receptor-associated protein (GABARAP), microtubule-associated protein 1 light chain 3B (LC3B) and autophagy-related protein 3 (ATG3), the radical scavenger activity-related metallothionein-1X (MT1X) gene and the anti-inflammatory activity-related annexin A2 (ANXA) gene. Resveratrol 10-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 208-212 24220335-3 2013 In this study, we found that the acetylation levels of phosphotidylethanolamine (PE)-conjugated LC3B (LC3B-II), which is a critical component of double-membrane autophagosome, were profoundly decreased in HeLa cells upon autophagy induction by serum starvation. phosphotidylethanolamine 55-79 microtubule associated protein 1 light chain 3 beta Homo sapiens 96-100 24220335-3 2013 In this study, we found that the acetylation levels of phosphotidylethanolamine (PE)-conjugated LC3B (LC3B-II), which is a critical component of double-membrane autophagosome, were profoundly decreased in HeLa cells upon autophagy induction by serum starvation. phosphotidylethanolamine 55-79 microtubule associated protein 1 light chain 3 beta Homo sapiens 102-109 24220335-3 2013 In this study, we found that the acetylation levels of phosphotidylethanolamine (PE)-conjugated LC3B (LC3B-II), which is a critical component of double-membrane autophagosome, were profoundly decreased in HeLa cells upon autophagy induction by serum starvation. pe 81-83 microtubule associated protein 1 light chain 3 beta Homo sapiens 96-100 24220335-3 2013 In this study, we found that the acetylation levels of phosphotidylethanolamine (PE)-conjugated LC3B (LC3B-II), which is a critical component of double-membrane autophagosome, were profoundly decreased in HeLa cells upon autophagy induction by serum starvation. pe 81-83 microtubule associated protein 1 light chain 3 beta Homo sapiens 102-109 24220335-5 2013 Under normal culture medium, we observed increased levels of acetylated LC3B-II in cells treated with tubacin, a specific inhibitor of histone deacetylase 6 (HDAC6). tubacin 102-109 microtubule associated protein 1 light chain 3 beta Homo sapiens 72-79 24220335-6 2013 However, tubacin only partially suppressed serum-starvation-induced LC3B-II deacetylation, suggesting that HDAC6 is not the only deacetylase acting on LC3B-II during serum-starvation-induced autophagy. tubacin 9-16 microtubule associated protein 1 light chain 3 beta Homo sapiens 68-75 24220335-7 2013 Interestingly, tubacin-induced increase in LC3B-II acetylation was associated with p62/SQSTM1 accumulation upon serum starvation. tubacin 15-22 microtubule associated protein 1 light chain 3 beta Homo sapiens 43-50 23939040-6 2013 Although piperine induced low levels of apoptosis, it promoted autophagy as evidenced by the increased level of LC3B-II and the formation of LC3B puncta in LNCaP and PC-3 cells. piperine 9-17 microtubule associated protein 1 light chain 3 beta Homo sapiens 112-116 23939040-6 2013 Although piperine induced low levels of apoptosis, it promoted autophagy as evidenced by the increased level of LC3B-II and the formation of LC3B puncta in LNCaP and PC-3 cells. piperine 9-17 microtubule associated protein 1 light chain 3 beta Homo sapiens 141-145 23939040-7 2013 The piperine-induced autophagic flux was further confirmed by assaying LC3-II accumulation and LC3B puncta formation in the presence of chloroquine, a well-known autophagy inhibitor. piperine 4-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 95-99 23939040-7 2013 The piperine-induced autophagic flux was further confirmed by assaying LC3-II accumulation and LC3B puncta formation in the presence of chloroquine, a well-known autophagy inhibitor. Chloroquine 136-147 microtubule associated protein 1 light chain 3 beta Homo sapiens 95-99 23805866-7 2013 Further mutational analysis suggests that the replacement of the canonical tryptophan residue side chain of autophagy receptors with the smaller phenylalanine side chain in optineurin significantly weakens its interaction with the autophagy modifier LC3B. Tryptophan 75-85 microtubule associated protein 1 light chain 3 beta Homo sapiens 250-254 23805866-7 2013 Further mutational analysis suggests that the replacement of the canonical tryptophan residue side chain of autophagy receptors with the smaller phenylalanine side chain in optineurin significantly weakens its interaction with the autophagy modifier LC3B. Phenylalanine 145-158 microtubule associated protein 1 light chain 3 beta Homo sapiens 250-254 24040206-0 2013 The carboxyl-terminal amino acids render pro-human LC3B migration similar to lipidated LC3B in SDS-PAGE. Sodium Dodecyl Sulfate 95-98 microtubule associated protein 1 light chain 3 beta Homo sapiens 87-91 24040206-4 2013 We found that pro-human LC3B migrated at similar rate as LC3B-II in SDS-PAGE. Sodium Dodecyl Sulfate 68-71 microtubule associated protein 1 light chain 3 beta Homo sapiens 24-28 24040206-5 2013 The carboxyl-terminal five amino acids, particularly Lysine(122) and Leucine(123) of human LC3B play a major role in the faster migration of unprocessed LC3B, rendering it indistinguishable from LC3B-II in Wb assays. Lysine 53-59 microtubule associated protein 1 light chain 3 beta Homo sapiens 91-95 24040206-5 2013 The carboxyl-terminal five amino acids, particularly Lysine(122) and Leucine(123) of human LC3B play a major role in the faster migration of unprocessed LC3B, rendering it indistinguishable from LC3B-II in Wb assays. Lysine 53-59 microtubule associated protein 1 light chain 3 beta Homo sapiens 153-157 24040206-5 2013 The carboxyl-terminal five amino acids, particularly Lysine(122) and Leucine(123) of human LC3B play a major role in the faster migration of unprocessed LC3B, rendering it indistinguishable from LC3B-II in Wb assays. Leucine 69-76 microtubule associated protein 1 light chain 3 beta Homo sapiens 91-95 23815914-6 2013 NAC or Tiron, ROS scavenger, could decrease the FTY720 induced apoptosis and the expression of LC3B-II was reduced in combination of FTY720 with NAC or Tiron as compared with treatment with FTY720 only. Reactive Oxygen Species 14-17 microtubule associated protein 1 light chain 3 beta Homo sapiens 95-99 23815914-6 2013 NAC or Tiron, ROS scavenger, could decrease the FTY720 induced apoptosis and the expression of LC3B-II was reduced in combination of FTY720 with NAC or Tiron as compared with treatment with FTY720 only. 1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt 152-157 microtubule associated protein 1 light chain 3 beta Homo sapiens 95-99 23585825-3 2013 Using CS extract (CSE) as a surrogate for CS, we found that it markedly increased the expressions of both LC3B-I and LC3B-II as well as autophagosomes in airway epithelial cells. Cesium 6-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 23645732-5 2013 The MAP1LC3B gene was synergistically overexpressed in MDA-MB-231 cells treated with CTet-cisplatin combination. ctet 85-89 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-12 23645732-5 2013 The MAP1LC3B gene was synergistically overexpressed in MDA-MB-231 cells treated with CTet-cisplatin combination. Cisplatin 90-99 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-12 23585825-3 2013 Using CS extract (CSE) as a surrogate for CS, we found that it markedly increased the expressions of both LC3B-I and LC3B-II as well as autophagosomes in airway epithelial cells. Cesium 6-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 117-121 23585825-3 2013 Using CS extract (CSE) as a surrogate for CS, we found that it markedly increased the expressions of both LC3B-I and LC3B-II as well as autophagosomes in airway epithelial cells. Cesium 18-20 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 23585825-3 2013 Using CS extract (CSE) as a surrogate for CS, we found that it markedly increased the expressions of both LC3B-I and LC3B-II as well as autophagosomes in airway epithelial cells. Cesium 18-20 microtubule associated protein 1 light chain 3 beta Homo sapiens 117-121 23585825-8 2013 Supplement of NAC (a GSH precursor) or GSH recapitulated the effect of Nrf2, suggesting the increase of cellular GSH level is responsible for Nrf2 effect on LC3B and autophagosome. Glutathione 21-24 microtubule associated protein 1 light chain 3 beta Homo sapiens 157-161 23585825-8 2013 Supplement of NAC (a GSH precursor) or GSH recapitulated the effect of Nrf2, suggesting the increase of cellular GSH level is responsible for Nrf2 effect on LC3B and autophagosome. Glutathione 39-42 microtubule associated protein 1 light chain 3 beta Homo sapiens 157-161 23585825-8 2013 Supplement of NAC (a GSH precursor) or GSH recapitulated the effect of Nrf2, suggesting the increase of cellular GSH level is responsible for Nrf2 effect on LC3B and autophagosome. Glutathione 39-42 microtubule associated protein 1 light chain 3 beta Homo sapiens 157-161 23841076-3 2013 Results show that, in addition to apoptosis, DHA increased the expression levels of lipidated form LC3B and potently stimulated the autophagic flux, suggesting that DHA induces both autophagy and apoptosis in cancer cells expressing mutant p53. Docosahexaenoic Acids 45-48 microtubule associated protein 1 light chain 3 beta Homo sapiens 99-103 23333919-7 2013 The p62/LC3b complex interacts with Fas and truncated BID (tBID) physically. ammonium ferrous sulfate 36-39 microtubule associated protein 1 light chain 3 beta Homo sapiens 8-12 23333919-7 2013 The p62/LC3b complex interacts with Fas and truncated BID (tBID) physically. tBID 59-63 microtubule associated protein 1 light chain 3 beta Homo sapiens 8-12 23354299-8 2013 This autophagic effect induced by sorafenib was validated by LC3B-I lipidation and autophagosome accumulation. Sorafenib 34-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 61-65 23333919-13 2013 Subsequently, the LC3b-mediated lysosomal degradation of tBID is eliminated. tBID 57-61 microtubule associated protein 1 light chain 3 beta Homo sapiens 18-22 23195224-6 2013 On the other hand, the lower mitochondrial function in QS was associated with an increase in Beclin, LC3B, Bnip3 and LAMP protein levels, indicating active mitophagy and lysosome biosynthesis processes. quercetin-5',8-disulfonate 55-57 microtubule associated protein 1 light chain 3 beta Homo sapiens 101-105 23209295-3 2013 Here we report that phosphorylation of serine residues 17 and 24 flanking the Bnip3 LIR promotes binding to specific Atg8 members LC3B and GATE-16. Serine 39-45 microtubule associated protein 1 light chain 3 beta Homo sapiens 130-134 23841076-3 2013 Results show that, in addition to apoptosis, DHA increased the expression levels of lipidated form LC3B and potently stimulated the autophagic flux, suggesting that DHA induces both autophagy and apoptosis in cancer cells expressing mutant p53. Docosahexaenoic Acids 165-168 microtubule associated protein 1 light chain 3 beta Homo sapiens 99-103 22917563-5 2012 Transfection of SH-SY5Y cells with AMPK or LC3beta shRNA, as well as treatment with pharmacological autophagy inhibitors suppressed, while mTOR inhibitor rapamycin potentiated 6-OHDA-induced oxidative stress and apoptotic cell death. Oxidopamine 176-182 microtubule associated protein 1 light chain 3 beta Homo sapiens 43-50 23817226-4 2013 Western blot analysis confirmed that simvastatin significantly increased protein expression of typical autophagy markers LC3B and Beclin1 in these CAMs. Simvastatin 37-48 microtubule associated protein 1 light chain 3 beta Homo sapiens 121-125 22922758-5 2012 Moreover, knockdown of CerS1 abrogated sodium selenite-induced mitophagy, and stable LC3B knockdown protected against CerS1- and C(18)-ceramide-dependent mitophagy and blocked tumor suppression in vivo. Ceramides 135-143 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 22249245-3 2012 Although a form of LC3B modified by phosphatidylethanolamine (form-II) is localized in autophagosomes, it is not clear whether other LC3 proteins also function in autophagy. phosphatidylethanolamine 36-60 microtubule associated protein 1 light chain 3 beta Homo sapiens 19-23 22922758-3 2012 C(18)-ceramide-induced lethal autophagy was regulated via microtubule-associated protein 1 light chain 3 beta-lipidation, forming LC3B-II, and selective targeting of mitochondria by LC3B-II-containing autophagolysosomes (mitophagy) through direct interaction between ceramide and LC3B-II upon Drp1-dependent mitochondrial fission, leading to inhibition of mitochondrial function and oxygen consumption. Ceramides 6-14 microtubule associated protein 1 light chain 3 beta Homo sapiens 58-109 22922758-6 2012 Thus, these data suggest a new receptor function of ceramide for anchoring LC3B-II autophagolysosomes to mitochondrial membranes, defining a key mechanism for the induction of lethal mitophagy. Ceramides 52-60 microtubule associated protein 1 light chain 3 beta Homo sapiens 75-79 22922758-3 2012 C(18)-ceramide-induced lethal autophagy was regulated via microtubule-associated protein 1 light chain 3 beta-lipidation, forming LC3B-II, and selective targeting of mitochondria by LC3B-II-containing autophagolysosomes (mitophagy) through direct interaction between ceramide and LC3B-II upon Drp1-dependent mitochondrial fission, leading to inhibition of mitochondrial function and oxygen consumption. Ceramides 6-14 microtubule associated protein 1 light chain 3 beta Homo sapiens 130-134 22922758-3 2012 C(18)-ceramide-induced lethal autophagy was regulated via microtubule-associated protein 1 light chain 3 beta-lipidation, forming LC3B-II, and selective targeting of mitochondria by LC3B-II-containing autophagolysosomes (mitophagy) through direct interaction between ceramide and LC3B-II upon Drp1-dependent mitochondrial fission, leading to inhibition of mitochondrial function and oxygen consumption. Ceramides 6-14 microtubule associated protein 1 light chain 3 beta Homo sapiens 182-186 22922758-3 2012 C(18)-ceramide-induced lethal autophagy was regulated via microtubule-associated protein 1 light chain 3 beta-lipidation, forming LC3B-II, and selective targeting of mitochondria by LC3B-II-containing autophagolysosomes (mitophagy) through direct interaction between ceramide and LC3B-II upon Drp1-dependent mitochondrial fission, leading to inhibition of mitochondrial function and oxygen consumption. Ceramides 6-14 microtubule associated protein 1 light chain 3 beta Homo sapiens 182-186 22922758-4 2012 Accordingly, expression of mutant LC3B with impaired ceramide binding, as predicted by molecular modeling, prevented CerS1-mediated mitochondrial targeting, recovering oxygen consumption. Ceramides 53-61 microtubule associated protein 1 light chain 3 beta Homo sapiens 34-38 22922758-4 2012 Accordingly, expression of mutant LC3B with impaired ceramide binding, as predicted by molecular modeling, prevented CerS1-mediated mitochondrial targeting, recovering oxygen consumption. Oxygen 168-174 microtubule associated protein 1 light chain 3 beta Homo sapiens 34-38 22784363-11 2012 Suppression of caspase cleavage by the pan caspase inhibitor, Z-VAD-fmk, suppressed induction of LC3-B and Atg12 and reduced autophagic cell death, indicating caspase activity was essential for autophagic cell death induced by helenalin. benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone 62-71 microtubule associated protein 1 light chain 3 beta Homo sapiens 97-102 22622129-5 2012 To quantify autophagy, we reasoned that GFP-tagged LC3B could serve as a time-resolved fluorescence resonance energy transfer (TR-FRET) acceptor upon cell lysis in the presence of terbium-labeled LC3B antibodies. Terbium 180-187 microtubule associated protein 1 light chain 3 beta Homo sapiens 51-55 22622129-5 2012 To quantify autophagy, we reasoned that GFP-tagged LC3B could serve as a time-resolved fluorescence resonance energy transfer (TR-FRET) acceptor upon cell lysis in the presence of terbium-labeled LC3B antibodies. Terbium 180-187 microtubule associated protein 1 light chain 3 beta Homo sapiens 196-200 22784363-11 2012 Suppression of caspase cleavage by the pan caspase inhibitor, Z-VAD-fmk, suppressed induction of LC3-B and Atg12 and reduced autophagic cell death, indicating caspase activity was essential for autophagic cell death induced by helenalin. helenalin 227-236 microtubule associated protein 1 light chain 3 beta Homo sapiens 97-102 22170153-7 2012 Overexpression of LC3B, ATG5 and ATG12 resulted in an improved mitochondrial membrane potential, enhanced ATP production and generated anti-apoptotic effects, while ROS levels remained unchanged and the amount of oxidized proteins increased. Adenosine Triphosphate 106-109 microtubule associated protein 1 light chain 3 beta Homo sapiens 18-22 22367066-5 2012 Inhibition of autophagy by 3-methyladenine or LC3B small interfering (siRNA) resulted in enhanced apoptotic cell death, suggesting that MEMC-induced autophagy functions as a suppressor of apoptosis. Mercury, chloro(2-methoxyethyl)- 148-152 microtubule associated protein 1 light chain 3 beta Homo sapiens 46-50 22803432-11 2012 RESULTS: Two h after culture, when compared with the EBSS + vehicle serum group, GFP-LC3B changing from diffused distribution to dotted distribution was obviously inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group (P<0.01). ebss 53-57 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 22803432-11 2012 RESULTS: Two h after culture, when compared with the EBSS + vehicle serum group, GFP-LC3B changing from diffused distribution to dotted distribution was obviously inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group (P<0.01). ebss 180-184 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 22803432-11 2012 RESULTS: Two h after culture, when compared with the EBSS + vehicle serum group, GFP-LC3B changing from diffused distribution to dotted distribution was obviously inhibited in the EBSS +5 times LR serum group and the EBSS +10 times LR serum group (P<0.01). ebss 180-184 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 22170153-7 2012 Overexpression of LC3B, ATG5 and ATG12 resulted in an improved mitochondrial membrane potential, enhanced ATP production and generated anti-apoptotic effects, while ROS levels remained unchanged and the amount of oxidized proteins increased. Reactive Oxygen Species 165-168 microtubule associated protein 1 light chain 3 beta Homo sapiens 18-22 22905241-4 2012 METHODOLOGY/PRINCIPAL FINDINGS: Analysis of gene expression microarray data and subsequent confirmation by quantitative real-time PCR, showed that CTet is able to induce up-regulation of key signaling molecules involved in endoplasmic reticulum (ER) stress response (e.g. DDIT3/CHOP, CHAC1, ATF3, HSPA5/BiP/GRP78, CEBPB, ASNS) and autophagy (e.g. MAP1LC3B), in both MCF-7 and MDA-MB-231 cell lines. ctet 147-151 microtubule associated protein 1 light chain 3 beta Homo sapiens 347-355 21871960-10 2012 Inhibition of autophagy with bafilomycin A1, 3-methyladenine and LC3beta shRNA, as well as AMPK inhibition with compound C or AMPK siRNA, markedly increased apoptotic death of simvastatin-treated U251 cells. Simvastatin 176-187 microtubule associated protein 1 light chain 3 beta Homo sapiens 65-72 22679478-6 2012 Moreover, exposure to H(2)S led to features characteristic of autophagy, including increased formation of LC3B(+) autophagic vacuoles and acidic vesicular organelles as determined by immunofluorescence and acridine orange staining, respectively. Hydrogen Sulfide 22-27 microtubule associated protein 1 light chain 3 beta Homo sapiens 106-110 22860102-4 2012 Combination treatment resulted in a significant enhancement of ROS production resulting in immense DNA damage, induction of autophagy analyzed by transmission electron microscope and increase in expression of autophagy marker LC3B, and culminated in cell death analyzed by cleaved caspase 3. ros 63-66 microtubule associated protein 1 light chain 3 beta Homo sapiens 226-230 22860102-7 2012 Immunohistochemical analysis of the tumor tissues from animals treated with WFA/Dox combination showed a significant reduction in cell proliferation and formation of microvessels accompanied by increased in LC3B level, cleaved caspase 3, and DNA damage. withaferin A 76-79 microtubule associated protein 1 light chain 3 beta Homo sapiens 207-211 22860102-7 2012 Immunohistochemical analysis of the tumor tissues from animals treated with WFA/Dox combination showed a significant reduction in cell proliferation and formation of microvessels accompanied by increased in LC3B level, cleaved caspase 3, and DNA damage. Doxorubicin 80-83 microtubule associated protein 1 light chain 3 beta Homo sapiens 207-211 21741467-7 2011 In contrast, the MEK inhibitor (U0126) was suppressed autophagy and the effect of p-PD on ERK1/2, Beclin-1 and LC3B proteins expression, except for mutant p53. U 0126 32-37 microtubule associated protein 1 light chain 3 beta Homo sapiens 111-115 22815878-5 2012 Furthermore, cytotrophoblasts cultured under hypoxia (2% oxygen) in the presence or absence of nutlin-3 (a p53 activity stimulator) had higher levels of LC3B-II, DRAM, and M30 proteins and increased Bax mRNA expression compared with controls cultured under standard conditions. Oxygen 57-63 microtubule associated protein 1 light chain 3 beta Homo sapiens 153-157 21949121-4 2011 Autophagy inhibition by siRNA against Beclin 1 or LC3B, or by chloroquine, partially reversed the cytoprotective effect of AZD8055 that was independent of cell cycle inhibition. (5-(2,4-bis((3S)-3-methylmorpholin-4-yl)pyrido(2,3-d)pyrimidin-7-yl)-2-methoxyphenyl)methanol 123-130 microtubule associated protein 1 light chain 3 beta Homo sapiens 50-54 21269334-9 2011 Western blotting for LC3B showed increased LC3B-I and LC3B-II expression after TMZ treatment. Temozolomide 79-82 microtubule associated protein 1 light chain 3 beta Homo sapiens 21-25 21441382-8 2011 Furthermore, CO-dependent induction of LC3B expression was inhibited by N-acetyl-L-cysteine and the mitochondria-targeting antioxidant, Mito-TEMPO. Acetylcysteine 72-91 microtubule associated protein 1 light chain 3 beta Homo sapiens 39-43 21269334-9 2011 Western blotting for LC3B showed increased LC3B-I and LC3B-II expression after TMZ treatment. Temozolomide 79-82 microtubule associated protein 1 light chain 3 beta Homo sapiens 43-47 21269334-9 2011 Western blotting for LC3B showed increased LC3B-I and LC3B-II expression after TMZ treatment. Temozolomide 79-82 microtubule associated protein 1 light chain 3 beta Homo sapiens 43-47 19671920-5 2009 DFO-treated cells show cytosolic accumulation of LC3B, a critical protein involved in autophagosomal-lysosomal degradation. Deferoxamine 0-3 microtubule associated protein 1 light chain 3 beta Homo sapiens 49-53 21667024-6 2011 Searching for the mechanism that underlies the effect of stromal cells on RAF265 response we found that stromal cells inhibited RAF265-induced increase in ERK1/2 phosphorylation, supported RAF265-dependent downregulation of PKCalpha (protein kinase Calpha) and prevented RAF265-induced conversion of LC3B, a marker of autophagy. RAF265 128-134 microtubule associated protein 1 light chain 3 beta Homo sapiens 300-304 20818167-5 2010 With the LC3B-PLA(2) substrate, we show that mutation of the glycine proximal to the scissile bond in LC3B abolishes activity. Glycine 61-68 microtubule associated protein 1 light chain 3 beta Homo sapiens 9-13 20818167-5 2010 With the LC3B-PLA(2) substrate, we show that mutation of the glycine proximal to the scissile bond in LC3B abolishes activity. Glycine 61-68 microtubule associated protein 1 light chain 3 beta Homo sapiens 102-106 20818167-6 2010 Both substrates showed high specificity for recombinant purified autophagin-1/Atg4B compared to closely related proteases and the LC3B-PLA(2) substrate afforded substantially higher catalytic rates (k(cat)/K(m) 5.26 x 10(5) M(-1)/sec(-1)) than Ac-GTFG-AFC peptide (0.92 M(-1)/sec(-1)), consistent with substrate-induced activation. Actinium 244-246 microtubule associated protein 1 light chain 3 beta Homo sapiens 130-134 20818167-6 2010 Both substrates showed high specificity for recombinant purified autophagin-1/Atg4B compared to closely related proteases and the LC3B-PLA(2) substrate afforded substantially higher catalytic rates (k(cat)/K(m) 5.26 x 10(5) M(-1)/sec(-1)) than Ac-GTFG-AFC peptide (0.92 M(-1)/sec(-1)), consistent with substrate-induced activation. gtfg 247-251 microtubule associated protein 1 light chain 3 beta Homo sapiens 130-134 21667024-6 2011 Searching for the mechanism that underlies the effect of stromal cells on RAF265 response we found that stromal cells inhibited RAF265-induced increase in ERK1/2 phosphorylation, supported RAF265-dependent downregulation of PKCalpha (protein kinase Calpha) and prevented RAF265-induced conversion of LC3B, a marker of autophagy. RAF265 74-80 microtubule associated protein 1 light chain 3 beta Homo sapiens 300-304 21667024-6 2011 Searching for the mechanism that underlies the effect of stromal cells on RAF265 response we found that stromal cells inhibited RAF265-induced increase in ERK1/2 phosphorylation, supported RAF265-dependent downregulation of PKCalpha (protein kinase Calpha) and prevented RAF265-induced conversion of LC3B, a marker of autophagy. RAF265 128-134 microtubule associated protein 1 light chain 3 beta Homo sapiens 300-304 21667024-6 2011 Searching for the mechanism that underlies the effect of stromal cells on RAF265 response we found that stromal cells inhibited RAF265-induced increase in ERK1/2 phosphorylation, supported RAF265-dependent downregulation of PKCalpha (protein kinase Calpha) and prevented RAF265-induced conversion of LC3B, a marker of autophagy. RAF265 128-134 microtubule associated protein 1 light chain 3 beta Homo sapiens 300-304 20514020-4 2010 MAP1LC3B (LC3B) is a key component of the autophagosomal membrane, and in this study we demonstrate that ATF4 facilitates autophagy through direct binding to a cyclic AMP response element binding site in the LC3B promoter, resulting in LC3B upregulation. Cyclic AMP 160-170 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-8 20514020-4 2010 MAP1LC3B (LC3B) is a key component of the autophagosomal membrane, and in this study we demonstrate that ATF4 facilitates autophagy through direct binding to a cyclic AMP response element binding site in the LC3B promoter, resulting in LC3B upregulation. Cyclic AMP 160-170 microtubule associated protein 1 light chain 3 beta Homo sapiens 4-8 20514020-4 2010 MAP1LC3B (LC3B) is a key component of the autophagosomal membrane, and in this study we demonstrate that ATF4 facilitates autophagy through direct binding to a cyclic AMP response element binding site in the LC3B promoter, resulting in LC3B upregulation. Cyclic AMP 160-170 microtubule associated protein 1 light chain 3 beta Homo sapiens 10-14 20514020-4 2010 MAP1LC3B (LC3B) is a key component of the autophagosomal membrane, and in this study we demonstrate that ATF4 facilitates autophagy through direct binding to a cyclic AMP response element binding site in the LC3B promoter, resulting in LC3B upregulation. Cyclic AMP 160-170 microtubule associated protein 1 light chain 3 beta Homo sapiens 10-14 20514020-5 2010 Previously, we have shown that Bortezomib-induced ATF4 stabilization, which then upregulated LC3B expression and had a critical role in activating autophagy, protecting cells from Bortezomib-induced cell death. Bortezomib 31-41 microtubule associated protein 1 light chain 3 beta Homo sapiens 93-97 19577553-9 2009 When the cells were pretreated with inhibitors 5-aza-CdR or U0126 for 24h, the effect of arsenite on ERK1/2, LC3B, Beclin-1 and DAPK proteins expression is suppressed. U 0126 60-65 microtubule associated protein 1 light chain 3 beta Homo sapiens 109-113 19815708-6 2009 Here, we show that sirolimus and paclitaxel differentially induce self-digesting autophagy in vascular endothelial cells with changes in expression of LC3B, p53, and Bcl-2, considerably suppressing re-endothelialization and revascularization. Sirolimus 19-28 microtubule associated protein 1 light chain 3 beta Homo sapiens 151-155 19815708-6 2009 Here, we show that sirolimus and paclitaxel differentially induce self-digesting autophagy in vascular endothelial cells with changes in expression of LC3B, p53, and Bcl-2, considerably suppressing re-endothelialization and revascularization. Paclitaxel 33-43 microtubule associated protein 1 light chain 3 beta Homo sapiens 151-155 19577553-9 2009 When the cells were pretreated with inhibitors 5-aza-CdR or U0126 for 24h, the effect of arsenite on ERK1/2, LC3B, Beclin-1 and DAPK proteins expression is suppressed. arsenite 89-97 microtubule associated protein 1 light chain 3 beta Homo sapiens 109-113 15355958-2 2004 Since human Atg4B was found to cleave the carboxyl terminus of MAP1LC3B in vitro, we hypothesized that this exposes its carboxyl-terminal Gly(120). Glycine 138-141 microtubule associated protein 1 light chain 3 beta Homo sapiens 63-71 19417138-8 2009 We show that induction of autophagy by Bortezomib is dependent on the proteasomal stabilisation of ATF4 and up-regulation of LC3B by ATF4. Bortezomib 39-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 125-129 19417138-9 2009 We show that ATF4 and LC3B play a critical role in activating autophagy and protecting cells from Bortezomib-induced cell death. Bortezomib 98-108 microtubule associated protein 1 light chain 3 beta Homo sapiens 22-26 15355958-1 2004 Human light chain 3/MAP1LC3B, an autophagosomal ortholog of yeast Atg8, is conjugated to phospholipid (PL) via ubiquitylation-like reactions mediated by human Atg7 and Atg3. Phospholipids 89-101 microtubule associated protein 1 light chain 3 beta Homo sapiens 20-28 15355958-1 2004 Human light chain 3/MAP1LC3B, an autophagosomal ortholog of yeast Atg8, is conjugated to phospholipid (PL) via ubiquitylation-like reactions mediated by human Atg7 and Atg3. Phospholipids 103-105 microtubule associated protein 1 light chain 3 beta Homo sapiens 20-28 15355958-3 2004 It was recently reported, however, that when Myc-MAP1LC3B-His is expressed in HEK293 cells, its carboxyl terminus is not cleaved. Histidine 58-61 microtubule associated protein 1 light chain 3 beta Homo sapiens 49-57 15355958-7 2004 To clarify this contradiction, we sought to determine whether the carboxyl terminus of MAP1LC3B is cleaved to expose Gly(120) for further ubiquitylation-like reactions. Glycine 117-120 microtubule associated protein 1 light chain 3 beta Homo sapiens 87-95 15355958-8 2004 When MAP1LC3B-3xFLAG and Myc-MAP1LC3B-His were expressed in HEK293 cells, their carboxyl termini were cleaved, whereas there was little cleavage of mutant proteins MAP1LC3B(G120A)-3xFLAG and Myc-MAP1LC3B(G120A)-His, containing Ala in place of Gly(120). Histidine 38-41 microtubule associated protein 1 light chain 3 beta Homo sapiens 29-37 15355958-8 2004 When MAP1LC3B-3xFLAG and Myc-MAP1LC3B-His were expressed in HEK293 cells, their carboxyl termini were cleaved, whereas there was little cleavage of mutant proteins MAP1LC3B(G120A)-3xFLAG and Myc-MAP1LC3B(G120A)-His, containing Ala in place of Gly(120). Histidine 38-41 microtubule associated protein 1 light chain 3 beta Homo sapiens 29-37 15355958-8 2004 When MAP1LC3B-3xFLAG and Myc-MAP1LC3B-His were expressed in HEK293 cells, their carboxyl termini were cleaved, whereas there was little cleavage of mutant proteins MAP1LC3B(G120A)-3xFLAG and Myc-MAP1LC3B(G120A)-His, containing Ala in place of Gly(120). Histidine 38-41 microtubule associated protein 1 light chain 3 beta Homo sapiens 29-37 15355958-9 2004 An in vitro assay showed that Gly(120) is essential for carboxyl-terminal cleavage by human Atg4B as well as for formation of the intermediates Atg7-MAP1LC3B (ubiquitin-activating enzyme-substrate) and Atg3-MAP1LC3B (ubiquitin carrier protein-substrate). Glycine 30-33 microtubule associated protein 1 light chain 3 beta Homo sapiens 149-157 15355958-9 2004 An in vitro assay showed that Gly(120) is essential for carboxyl-terminal cleavage by human Atg4B as well as for formation of the intermediates Atg7-MAP1LC3B (ubiquitin-activating enzyme-substrate) and Atg3-MAP1LC3B (ubiquitin carrier protein-substrate). Glycine 30-33 microtubule associated protein 1 light chain 3 beta Homo sapiens 207-215 15355958-12 2004 These results indicate that the carboxyl terminus of MAP1LC3B is cleaved to expose Gly(120) for further ubiquitylation-like reactions. Glycine 83-86 microtubule associated protein 1 light chain 3 beta Homo sapiens 53-61 34952334-7 2022 On the other hand, UNC1999 treatment caused the increasing of LC3B and P62 in all cells, suggested that siEZH2 and UNC1999 affect ARR cells autophagy through different mechanisms. UNC1999 19-26 microtubule associated protein 1 light chain 3 beta Homo sapiens 62-66 12740394-8 2003 The essential site for the distinct post-translation modification of MAP1LC3B is Lys-122 rather than the conserved Gly-120. Lysine 81-84 microtubule associated protein 1 light chain 3 beta Homo sapiens 69-77 12740394-8 2003 The essential site for the distinct post-translation modification of MAP1LC3B is Lys-122 rather than the conserved Gly-120. Glycine 115-118 microtubule associated protein 1 light chain 3 beta Homo sapiens 69-77 33769048-3 2021 In this study, we show that the homologous members of the human Atg8 family proteins, LC3A and LC3B, are druggable by a small molecule inhibitor novobiocin. Novobiocin 145-155 microtubule associated protein 1 light chain 3 beta Homo sapiens 95-99 34913075-13 2022 Furthermore, niclosamide upregulated protein expression of cleaved caspase-3 and LC3B, while downregulated those of Bcl-2 and p62, in a dose-dependent manner in both Jurkat and CCRF-CEM cells. Niclosamide 13-24 microtubule associated protein 1 light chain 3 beta Homo sapiens 81-85 34352527-6 2022 Additionally, the results revealed that autophagy occurred in DPHP-triggered NETs formation, manifested as enhanced LC3B protein expressions and reduced p62 protein expressions. dphp 62-66 microtubule associated protein 1 light chain 3 beta Homo sapiens 116-120 34826546-4 2022 We found that iron, manifesting as ferric ammonium citrate (FAC; 0.05-4.8 mM), significantly inhibited cell growth, induced oxidative stress through the Fenton reaction, and functionally activated autophagy in EuESCs, as measured by 5-ethynyl-2"-deoxyuridine incorporation assay, MitoSOX Red staining, LC3 turnover assay, and tandem mCherry-eGFP-LC3B fluorescence microscopy. Iron 14-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 347-351 34849158-5 2022 Flow cytometry and western blot analysis were used to detect the effects of Fas on cell apoptosis and to determine the expression levels of autophagy-related proteins, LC3B and Beclin 1, induced by Neo. Neomycin 198-201 microtubule associated protein 1 light chain 3 beta Homo sapiens 168-172 34917923-5 2022 Moreover, although chloroquine and ivermectin effectively inhibited the expression of LC3B in the nucleus, chloroquine specifically maintained the performance of LC3B in cytoplasm, thereby contributing to the differentiation of ciliated cells and subsequent improvement in the beating functions of the cilia, whereas ivermectin only facilitated differentiation of goblet cells. Chloroquine 19-30 microtubule associated protein 1 light chain 3 beta Homo sapiens 86-90 34924126-6 2022 Mechanistically, Pt treatment efficiently enhanced mitophagy and upregulated PINK1, Parkin, and LC3B expression. pterostilbene 17-19 microtubule associated protein 1 light chain 3 beta Homo sapiens 96-100 34848192-8 2022 The autophagy indicators as MAP1LC3B cleavage and MAP1LC3B puncta were increased after AUY922 treatment. 5-(2,4-dihydroxy-5-isopropylphenyl)-4-(4-morpholin-4-ylmethylphenyl)isoxazole-3-carboxylic acid ethylamide 87-93 microtubule associated protein 1 light chain 3 beta Homo sapiens 28-36 34848192-8 2022 The autophagy indicators as MAP1LC3B cleavage and MAP1LC3B puncta were increased after AUY922 treatment. 5-(2,4-dihydroxy-5-isopropylphenyl)-4-(4-morpholin-4-ylmethylphenyl)isoxazole-3-carboxylic acid ethylamide 87-93 microtubule associated protein 1 light chain 3 beta Homo sapiens 50-58 34917923-5 2022 Moreover, although chloroquine and ivermectin effectively inhibited the expression of LC3B in the nucleus, chloroquine specifically maintained the performance of LC3B in cytoplasm, thereby contributing to the differentiation of ciliated cells and subsequent improvement in the beating functions of the cilia, whereas ivermectin only facilitated differentiation of goblet cells. Ivermectin 35-45 microtubule associated protein 1 light chain 3 beta Homo sapiens 86-90 34917923-5 2022 Moreover, although chloroquine and ivermectin effectively inhibited the expression of LC3B in the nucleus, chloroquine specifically maintained the performance of LC3B in cytoplasm, thereby contributing to the differentiation of ciliated cells and subsequent improvement in the beating functions of the cilia, whereas ivermectin only facilitated differentiation of goblet cells. Chloroquine 107-118 microtubule associated protein 1 light chain 3 beta Homo sapiens 162-166 34831341-0 2021 Direct Interaction of ATP7B and LC3B Proteins Suggests a Cooperative Role of Copper Transportation and Autophagy. Copper 77-83 microtubule associated protein 1 light chain 3 beta Homo sapiens 32-36 34943939-6 2021 High concentrations of PBCA inhibited MAP1LC3B/GABARAP lipidation and LC3 flux, and blocked bulk autophagic cargo flux induced by mTOR inhibition. Enbucrilate 23-27 microtubule associated protein 1 light chain 3 beta Homo sapiens 38-46 34151717-4 2021 The results showed that 1 mM Bupivacaine was available to signally inhibit CRC cell vitality, promoted apoptosis rate and apoptosis gene expression, like Bax, and caspase-3, inhibited Bcl-2 expression, inhibited cancer cell migration, promoted autophagy-related protein LC3B II/LC3B I ratio and beclin-1 expression, and inhibited p62 expression. Bupivacaine 29-40 microtubule associated protein 1 light chain 3 beta Homo sapiens 278-282 34829701-10 2021 Moreover, light chain 3B (LC3B)-II accumulation and enhanced p62 expression indicated that autophagy occurred in the shikonin-treated group. shikonin 117-125 microtubule associated protein 1 light chain 3 beta Homo sapiens 10-24 34829701-10 2021 Moreover, light chain 3B (LC3B)-II accumulation and enhanced p62 expression indicated that autophagy occurred in the shikonin-treated group. shikonin 117-125 microtubule associated protein 1 light chain 3 beta Homo sapiens 26-30 34829701-11 2021 LC3B knockdown considerably recovered cell viability in the presence of shikonin. shikonin 72-80 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-4 34726962-6 2022 Furthermore, the administration of higher concentration of DACi was able to down-regulate the protein level of Beclin1 and p62 and to induce the conversion of LC3B-I into the active LC3B-II form, thus confirming an active autophagic process. daci 59-63 microtubule associated protein 1 light chain 3 beta Homo sapiens 159-163 34831341-10 2021 These findings suggest a cooperative role of ATP7B and LC3B in autophagy-mediated copper clearance. Copper 82-88 microtubule associated protein 1 light chain 3 beta Homo sapiens 55-59 34966970-7 2021 Treatment with 2 microM triethylsilyl resveratrol for 72 h led to a significant (p < 0.05) increase in GFP-LC3B labelling in HuH7 cells compared to the control cells. triethylsilyl resveratrol 24-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 107-111 34740308-6 2021 We show that CBK79 induces non-canonical lipidation of MAP1LC3B/LC3B (microtubule-associated protein 1 light chain 3 beta) that requires ATG16L1 but is independent of the ULK1 (unc-51 like autophagy activating kinase 1) and class III phosphatidylinositol 3-kinase (PtdIns3K) complexes. cbk79 13-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 55-63 34740308-6 2021 We show that CBK79 induces non-canonical lipidation of MAP1LC3B/LC3B (microtubule-associated protein 1 light chain 3 beta) that requires ATG16L1 but is independent of the ULK1 (unc-51 like autophagy activating kinase 1) and class III phosphatidylinositol 3-kinase (PtdIns3K) complexes. cbk79 13-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 64-68 34740308-6 2021 We show that CBK79 induces non-canonical lipidation of MAP1LC3B/LC3B (microtubule-associated protein 1 light chain 3 beta) that requires ATG16L1 but is independent of the ULK1 (unc-51 like autophagy activating kinase 1) and class III phosphatidylinositol 3-kinase (PtdIns3K) complexes. cbk79 13-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 70-121 34668631-8 2021 In vitro, in vivo and clinical investigations further confirmed that the miR-99a-3p agomir reduced the expression of EIF4EBP1, LC3B and LAMP-2A. mir-99a-3p 73-83 microtubule associated protein 1 light chain 3 beta Homo sapiens 127-131 34455243-8 2021 Under AAPH-induced oxidative stress, increased fluorescence intensity of Parkin, increased expression of PINK1, Parkin, and LC3B-II, and decreased expression of MFN2 and p62 were observed, whereas the opposite effects were induced under alpha-tocopherol treatment. 2,2'-azobis(2-amidinopropane) 6-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 124-128 34589147-10 2021 Notably, itraconazole induced autophagy by enhancing LC3B and p62 expression. Itraconazole 9-21 microtubule associated protein 1 light chain 3 beta Homo sapiens 53-57 34834228-6 2021 We also showed that GA was more effective in upregulating the production of ER stress marker GRP78, autophagy marker LC3B-II, and differentiation markers involucrin and filaggrin in A431 cells than in HaCaT. gibberellic acid 20-22 microtubule associated protein 1 light chain 3 beta Homo sapiens 117-121 34455243-8 2021 Under AAPH-induced oxidative stress, increased fluorescence intensity of Parkin, increased expression of PINK1, Parkin, and LC3B-II, and decreased expression of MFN2 and p62 were observed, whereas the opposite effects were induced under alpha-tocopherol treatment. alpha-Tocopherol 237-253 microtubule associated protein 1 light chain 3 beta Homo sapiens 124-128 34510030-5 2021 BA-induced autophagy was evidenced by epifluorescence imaging of lentivirus-induced expression of mCherry-GFP-LC3B and increased expression of two autophagy-related proteins, LC3B-II and TEM. betulinic acid 0-2 microtubule associated protein 1 light chain 3 beta Homo sapiens 110-114 34681608-7 2021 BPA induced neurotoxicity by down-regulating Bcl-2 and initiating apoptosis and autophagy flux inhibition (featured by nuclear translocation of apoptosis-inducing factor (AIF), light chain 3B (LC3B) aggregation, and p62 accumulation). bisphenol A 0-3 microtubule associated protein 1 light chain 3 beta Homo sapiens 177-191 34681608-7 2021 BPA induced neurotoxicity by down-regulating Bcl-2 and initiating apoptosis and autophagy flux inhibition (featured by nuclear translocation of apoptosis-inducing factor (AIF), light chain 3B (LC3B) aggregation, and p62 accumulation). bisphenol A 0-3 microtubule associated protein 1 light chain 3 beta Homo sapiens 193-197 34343907-13 2021 Finally, specific and non-toxic production of H2O2 by the GOX/CAT system as evidenced by elevated peroxiredoxin (Prx-2) also induced LC3B which was efficiently blocked by NAC. Hydrogen Peroxide 46-50 microtubule associated protein 1 light chain 3 beta Homo sapiens 133-137 34343907-13 2021 Finally, specific and non-toxic production of H2O2 by the GOX/CAT system as evidenced by elevated peroxiredoxin (Prx-2) also induced LC3B which was efficiently blocked by NAC. Acetylcysteine 171-174 microtubule associated protein 1 light chain 3 beta Homo sapiens 133-137 34510030-5 2021 BA-induced autophagy was evidenced by epifluorescence imaging of lentivirus-induced expression of mCherry-GFP-LC3B and increased expression of two autophagy-related proteins, LC3B-II and TEM. betulinic acid 0-2 microtubule associated protein 1 light chain 3 beta Homo sapiens 175-179 34440609-6 2021 The increased level of LC3B-II induced by CB-2 was neither eliminated when autophagy initiation was suppressed by wortmannin nor further increased when autophagosome degradation was inhibited by chloroquine (CQ). Wortmannin 114-124 microtubule associated protein 1 light chain 3 beta Homo sapiens 23-27 34146643-4 2021 And chloroquine treatment increased LC3B levels in ILK-knockdown GES-1 cells. Chloroquine 4-15 microtubule associated protein 1 light chain 3 beta Homo sapiens 36-40 34571747-10 2021 Immunofluorescence evidenced the accumulation of LC3B puncta in U87Mg cells pretreated with autophagy inhibitor Bafilomycin A1. bafilomycin A1 112-126 microtubule associated protein 1 light chain 3 beta Homo sapiens 49-53 34437575-12 2021 The interaction of etoposide with pertuzumab or trastuzumab induced programmed cell death via extrinsic and intrinsic apoptotic pathways in AGS gastric cancer cells, but did not affect autophagy, where a decrease of Beclin-1, LC3A and LC3B was observed in comparison with the untreated control. Etoposide 19-28 microtubule associated protein 1 light chain 3 beta Homo sapiens 235-239 34440609-6 2021 The increased level of LC3B-II induced by CB-2 was neither eliminated when autophagy initiation was suppressed by wortmannin nor further increased when autophagosome degradation was inhibited by chloroquine (CQ). Chloroquine 195-206 microtubule associated protein 1 light chain 3 beta Homo sapiens 23-27 34440609-6 2021 The increased level of LC3B-II induced by CB-2 was neither eliminated when autophagy initiation was suppressed by wortmannin nor further increased when autophagosome degradation was inhibited by chloroquine (CQ). Chloroquine 208-210 microtubule associated protein 1 light chain 3 beta Homo sapiens 23-27 34099041-3 2021 RESULTS: We found that Spermidine (SPD) significantly increased protein expression of autophagy markers microtubule-associated protein 1 light chain 3 beta-II (MAP1LC3B-II/LC3B-II) and sequestosome-1/p62 (SQSTM1/p62), and evoked autophagic flux in FGSCs. Spermidine 23-33 microtubule associated protein 1 light chain 3 beta Homo sapiens 160-168 34399755-11 2021 Animal experiments showed that the tumor volume of metformin group was smaller, meanwhile, the levels of p-AMPK (Thr172) and LC3B were up-regulated and the Ki-67 level was down-regulated, without abnormalities in biochemical indicators. Metformin 51-60 microtubule associated protein 1 light chain 3 beta Homo sapiens 125-129 34434111-7 2021 Besides, metformin increased the expression levels of phosphorylated adenosine 5"-monophosphate (AMP)-activated protein kinase (p-AMPK), microtubule-associated protein (MAP) light chain 3B (LC3B) and Beclin1 proteins, and reduced levels of phosphorylated mammalian target of rapamycin (p-mTOR) and p62 proteins in vivo and in vitro. Metformin 9-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 174-188 34434111-7 2021 Besides, metformin increased the expression levels of phosphorylated adenosine 5"-monophosphate (AMP)-activated protein kinase (p-AMPK), microtubule-associated protein (MAP) light chain 3B (LC3B) and Beclin1 proteins, and reduced levels of phosphorylated mammalian target of rapamycin (p-mTOR) and p62 proteins in vivo and in vitro. Metformin 9-18 microtubule associated protein 1 light chain 3 beta Homo sapiens 190-194 34452418-7 2021 Besides that, rapamycin could disturb the expression of p62 and LC3B-II, and the transcription level of SCRV nucleoprotein mRNA. Sirolimus 14-23 microtubule associated protein 1 light chain 3 beta Homo sapiens 64-68 34361036-7 2021 Interestingly, except for KLE cells, hinokitiol induced autophagy by promoting the accumulation of the microtubule-associated protein light chain 3B (LC3B) and reducing the sequestosome-1 (p62/SQSTM1) protein level. beta-thujaplicin 37-47 microtubule associated protein 1 light chain 3 beta Homo sapiens 150-154 34395543-8 2021 Further studies demonstrated that penicilliumin B enhanced autophagic flux, promoted the activation of multiple autophagy-related proteins, such as mTOR, Beclin-1, ATG5, PINK1, and LC3B, and induced the degradation of p62. Penicilliumin B 34-49 microtubule associated protein 1 light chain 3 beta Homo sapiens 181-185 34443546-6 2021 Moreover, PA inhibits NF-kappaB and AKT/mTOR signaling pathways and induces LC3B protein levels, resulting in autophagy. propionic acid 10-12 microtubule associated protein 1 light chain 3 beta Homo sapiens 76-80 34467729-8 2021 The comparison with the control group revealed that galangin inhibited the viability of MGC803 cells, up-regulated the protein expression of microtuble-associated protein 1 light chain 3 B(LC3 B) II, inhibited the phosphorylation of NF-kappaB pathway-related proteins, and promoted the formation of autophagosomes in MGC803 cells. galangin 52-60 microtubule associated protein 1 light chain 3 beta Homo sapiens 189-194 34225916-2 2021 Microtubule-associated proteins 1A/1B light chain 3B (hereafter referred to as LC3) plays a crucial role during autophagosome formation, as cleavage of its immature form and subsequent conjugation to phosphatidylethanolamine facilitates autophagosomal membrane biogenesis. phosphatidylethanolamine 200-224 microtubule associated protein 1 light chain 3 beta Homo sapiens 0-52 34095303-8 2021 Our results showed that cisplatin gradually reduced the viable cell number over time, induced mitochondrial depolarization, decreased intracellular ATP concentration, and enhanced the expression of PINK1, Parkin, BNIP3, p62, and LC3B. Cisplatin 24-33 microtubule associated protein 1 light chain 3 beta Homo sapiens 229-233 34077031-12 2021 Western blot analysis showed that the expression of LC3B II increased while of p62 decreased remarkably upon treatment with Withaferin-A, suggestive of autophagic cell death. withaferin A 124-136 microtubule associated protein 1 light chain 3 beta Homo sapiens 52-56 34093169-7 2021 Furthermore, dapagliflozin increased the expression of the autophagy-related markers LC3B and Beclin1, in parallel with a drop in p62 level. dapagliflozin 13-26 microtubule associated protein 1 light chain 3 beta Homo sapiens 85-89 35636179-12 2022 PGG induced apoptosis and autophagy in NPC cells and elevated the Bax/Bcl-2 ratio and the protein levels of LC3B. pentagalloylglucose 0-3 microtubule associated protein 1 light chain 3 beta Homo sapiens 108-112 35490984-14 2022 With the use of V5-Nrf2 overexpression plasmid, Nrf2 siRNA and pathway inhibitors, we found NaHS activates the expressions of Nrf2 and PPAR-gamma, and inhibites ferritinophagy makers LC3B, NCOA4 and FTH1 in BEAS-2B cells. sodium bisulfide 92-96 microtubule associated protein 1 light chain 3 beta Homo sapiens 183-187 35460941-5 2022 The results showed, briefly, an exposure to niclosamide and quinacrine led to an increased apoptosis-related protein p53, cleaved caspase-3 and cleaved PARP and autophagy-related protein LC3B expression, and a decreased expression of autophagy-related protein p62, finally leading to cell apoptosis and autophage. Niclosamide 44-55 microtubule associated protein 1 light chain 3 beta Homo sapiens 187-191 35460941-5 2022 The results showed, briefly, an exposure to niclosamide and quinacrine led to an increased apoptosis-related protein p53, cleaved caspase-3 and cleaved PARP and autophagy-related protein LC3B expression, and a decreased expression of autophagy-related protein p62, finally leading to cell apoptosis and autophage. Quinacrine 60-70 microtubule associated protein 1 light chain 3 beta Homo sapiens 187-191 35431285-6 2022 LicB treatment not only decreased the levels of Bcl-2, p62, Caspase-3, and Ki67 protein in MG-63 and U2OS cell lines but also increased the levels of Cleaved Caspase-3, Beclin1, Bax, Atg7, and LC3B. licochalcone B 0-4 microtubule associated protein 1 light chain 3 beta Homo sapiens 193-197 35551547-6 2022 Lowest cell proliferation was achieved with the concomitant use of genomic DNA, HGFR inhibitor, and chloroquine, when the proliferation stimulating effect of STAT3 overexpression could be outweighed by the inhibitory effect of LC3B, indicating the putative involvement of HGFR-mTOR-ULK1 molecular cascade in HGFR inhibitor-mediated autophagy. Chloroquine 100-111 microtubule associated protein 1 light chain 3 beta Homo sapiens 227-231 35600978-0 2023 Zinc oxide nanoparticles inhibit osteosarcoma metastasis by downregulating beta-catenin via HIF-1alpha/BNIP3/LC3B-mediated mitophagy pathway. Zinc Oxide 0-10 microtubule associated protein 1 light chain 3 beta Homo sapiens 109-113 35600978-6 2023 We further proved that Zn2+ released from ZnO NPs induced downregulation of beta-catenin expression via HIF-1alpha/BNIP3/LC3B-mediated mitophagy pathway. Zinc 23-27 microtubule associated protein 1 light chain 3 beta Homo sapiens 121-125 35600978-6 2023 We further proved that Zn2+ released from ZnO NPs induced downregulation of beta-catenin expression via HIF-1alpha/BNIP3/LC3B-mediated mitophagy pathway. Zinc Oxide 42-45 microtubule associated protein 1 light chain 3 beta Homo sapiens 121-125 35600978-9 2023 In summary, our current study not only proved that ZnO NPs can inhibit OS metastasis by degrading beta-catenin in HIF-1alpha/BNIP3/LC3B-mediated mitophagy pathway, but also provided a far-reaching potential of ZnO NPs in clinical OS treatment with metastasis. Zinc Oxide 51-54 microtubule associated protein 1 light chain 3 beta Homo sapiens 131-135 35600978-9 2023 In summary, our current study not only proved that ZnO NPs can inhibit OS metastasis by degrading beta-catenin in HIF-1alpha/BNIP3/LC3B-mediated mitophagy pathway, but also provided a far-reaching potential of ZnO NPs in clinical OS treatment with metastasis. Zinc Oxide 210-213 microtubule associated protein 1 light chain 3 beta Homo sapiens 131-135 35571705-10 2022 Further, AdPlus-mCherry-GFP-LC3B adenovirus transfection was used to detect the effect of ursolic acid on the autophagic flow of 786-O cells; ursolic acid was combined with the autophagy inhibitor chloroquine (CQ) to detect the expression level of autophagy protein LC3II by Western blot. ursolic acid 90-102 microtubule associated protein 1 light chain 3 beta Homo sapiens 28-32 35240195-3 2022 The expression levels of Sirt3, Foxo3a, PINK1, Parkin and LC3B in RPE subjected to high-glucose (HG, 30 mM D-glucose) conditions were detected by RT-PCR and western blotting. Glucose 88-95 microtubule associated protein 1 light chain 3 beta Homo sapiens 58-62 35240195-3 2022 The expression levels of Sirt3, Foxo3a, PINK1, Parkin and LC3B in RPE subjected to high-glucose (HG, 30 mM D-glucose) conditions were detected by RT-PCR and western blotting. Glucose 109-116 microtubule associated protein 1 light chain 3 beta Homo sapiens 58-62 35414338-6 2022 Besides, the double silencing of LC3A and LC3B proteins was seen to decrease CCCP-induced mitophagy. Carbonyl Cyanide m-Chlorophenyl Hydrazone 77-81 microtubule associated protein 1 light chain 3 beta Homo sapiens 42-46 35339877-5 2022 Furthermore, Mfn2 overexpression ameliorated Cu-induced MAM dysfunction, and increased Cu-evoked autophagy in duck renal tubular epithelial cells accompanied with the elevation of autophagosomes number, ROS level, LC3 puncta, Atg5 and LC3B mRNA levels, and Beclin1, Atg14, LC3BII/LC3BI protein levels. Copper 87-89 microtubule associated protein 1 light chain 3 beta Homo sapiens 235-239 35456915-9 2022 Les-3331 decreased LC3A, LC3B, and Beclin-1 concentration in tested cell lines. les-3331 0-8 microtubule associated protein 1 light chain 3 beta Homo sapiens 25-29 35186935-0 2022 Plasmodium Circumsporozoite Protein Enhances the Efficacy of Gefitinib in Lung Adenocarcinoma Cells by Inhibiting Autophagy via Proteasomal Degradation of LC3B. Gefitinib 61-70 microtubule associated protein 1 light chain 3 beta Homo sapiens 155-159 35387352-4 2022 The inhibition of autophagy by silencing the LC3B gene reduced apoptosis, while blockade of autophagy flux using its inhibitor, Bafilomycin A1, further induced apoptosis upon pitavastatin treatment, which suggested that autophagy flux blockage was the cause of apoptosis by pitavastatin. pitavastatin 175-187 microtubule associated protein 1 light chain 3 beta Homo sapiens 45-49 35258392-6 2022 Hence, cells acquired mitochondria during evolution to profit from oxidative metabolism, but also built in an autophagy-based ROS-induced protective mechanism to guard against oxidative stress associated with OXPHOS function during quiescence.Abbreviations: AMPK: AMP-activated protein kinase; AOX: alternative oxidase; Baf A: bafilomycin A1; CI, respiratory complexes I; DCF-DA: 2",7"-dichlordihydrofluorescein diacetate; DHE: dihydroethidium; DSS: dextran sodium sulfate; DeltaPsimi: mitochondrial inner membrane potential; EdU: 5-ethynyl-2"-deoxyuridine; ETC: electron transport chain; FA: formaldehyde; HUVEC; human umbilical cord endothelial cells; IBD: inflammatory bowel disease; LC3B: microtubule associated protein 1 light chain 3 beta; LPS: lipopolysaccharide; MEFs: mouse embryonic fibroblasts; MTORC1: mechanistic target of rapamycin kinase complex 1; mtDNA: mitochondrial DNA; NAC: N-acetyl cysteine; OXPHOS: oxidative phosphorylation; PCs: proliferating cells; PE: phosphatidylethanolamine; PEITC: phenethyl isothiocyanate; QCs: quiescent cells; ROS: reactive oxygen species; PLA2: phospholipase A2, WB: western blot. ros 126-129 microtubule associated protein 1 light chain 3 beta Homo sapiens 687-691 35258392-6 2022 Hence, cells acquired mitochondria during evolution to profit from oxidative metabolism, but also built in an autophagy-based ROS-induced protective mechanism to guard against oxidative stress associated with OXPHOS function during quiescence.Abbreviations: AMPK: AMP-activated protein kinase; AOX: alternative oxidase; Baf A: bafilomycin A1; CI, respiratory complexes I; DCF-DA: 2",7"-dichlordihydrofluorescein diacetate; DHE: dihydroethidium; DSS: dextran sodium sulfate; DeltaPsimi: mitochondrial inner membrane potential; EdU: 5-ethynyl-2"-deoxyuridine; ETC: electron transport chain; FA: formaldehyde; HUVEC; human umbilical cord endothelial cells; IBD: inflammatory bowel disease; LC3B: microtubule associated protein 1 light chain 3 beta; LPS: lipopolysaccharide; MEFs: mouse embryonic fibroblasts; MTORC1: mechanistic target of rapamycin kinase complex 1; mtDNA: mitochondrial DNA; NAC: N-acetyl cysteine; OXPHOS: oxidative phosphorylation; PCs: proliferating cells; PE: phosphatidylethanolamine; PEITC: phenethyl isothiocyanate; QCs: quiescent cells; ROS: reactive oxygen species; PLA2: phospholipase A2, WB: western blot. ros 126-129 microtubule associated protein 1 light chain 3 beta Homo sapiens 693-744 35196199-11 2022 Our study shows that treatments targeting pathways to enhance autophagy have the potential for treating early AMD and provide support for the use of metformin, which has been found to reduce the risk of AMD development in human patients.Abbreviations:AMD: age-related macular degeneration; AMPK: 5" adenosine monophosphate-activated protein kinase APOE: apolipoprotein E; ATM: ataxia telangiectasia mutated; BCL2L1/Bcl-xL: BCL2-like 1; DAPI: 4"-6-diamidino-2-phenylindole; ERG: electroretinogram; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GCL: ganglion cell layer; INL: inner nuclear layer; IPL: inner plexiform layer; IS/OS: inner and outer photoreceptor segments; LAMP1: lysosomal-associated membrane protein 1; MAP1LC3B/LC3: microtubule-associated protein 1 light chain 3 beta; MTOR: mechanistic target of rapamycin kinase; OCT: optical coherence tomography; ONL: outer nuclear layer; OPs: oscillatory potentials; p-EIF4EBP1: phosphorylated eukaryotic translation initiation factor 4E binding protein 1; p-MAPK14/p38: phosphorylated mitogen-activated protein kinase 14; RPE: retinal pigment epithelium; RPS6KB/p70 S6 kinase: ribosomal protein S6 kinase; SQSTM1/p62: sequestosome 1; TP53/TRP53/p53: tumor related protein 53; TSC2: TSC complex subunit 2; WT: wild type. Metformin 149-158 microtubule associated protein 1 light chain 3 beta Homo sapiens 721-729 35196199-11 2022 Our study shows that treatments targeting pathways to enhance autophagy have the potential for treating early AMD and provide support for the use of metformin, which has been found to reduce the risk of AMD development in human patients.Abbreviations:AMD: age-related macular degeneration; AMPK: 5" adenosine monophosphate-activated protein kinase APOE: apolipoprotein E; ATM: ataxia telangiectasia mutated; BCL2L1/Bcl-xL: BCL2-like 1; DAPI: 4"-6-diamidino-2-phenylindole; ERG: electroretinogram; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GCL: ganglion cell layer; INL: inner nuclear layer; IPL: inner plexiform layer; IS/OS: inner and outer photoreceptor segments; LAMP1: lysosomal-associated membrane protein 1; MAP1LC3B/LC3: microtubule-associated protein 1 light chain 3 beta; MTOR: mechanistic target of rapamycin kinase; OCT: optical coherence tomography; ONL: outer nuclear layer; OPs: oscillatory potentials; p-EIF4EBP1: phosphorylated eukaryotic translation initiation factor 4E binding protein 1; p-MAPK14/p38: phosphorylated mitogen-activated protein kinase 14; RPE: retinal pigment epithelium; RPS6KB/p70 S6 kinase: ribosomal protein S6 kinase; SQSTM1/p62: sequestosome 1; TP53/TRP53/p53: tumor related protein 53; TSC2: TSC complex subunit 2; WT: wild type. Metformin 149-158 microtubule associated protein 1 light chain 3 beta Homo sapiens 735-786 35186935-12 2022 In the OE-CSP group, autophagy was significantly inhibited, and there was a decrease in LC3B protein after exposure to gefitinib. Gefitinib 119-128 microtubule associated protein 1 light chain 3 beta Homo sapiens 88-92 35186935-15 2022 CSP promoted ubiquitin-proteasome degradation of LC3B, leading to inhibition of autophagy in LUAD cells after treatment with gefitinib. Gefitinib 125-134 microtubule associated protein 1 light chain 3 beta Homo sapiens 49-53 35186935-17 2022 Conclusion: CSP can inhibit gefitinib-induced autophagy via proteasomal degradation of LC3B, which suggests that CSP could be used as an autophagy inhibitor to sensitize EGFR-TKIs. Gefitinib 28-37 microtubule associated protein 1 light chain 3 beta Homo sapiens 87-91 34997858-12 2022 Anlotinib induced autophagy in glioblastoma cells by increasing Beclin-1 and microtubule-associated protein 1 light chain 3B (LC3B) levels. anlotinib 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 126-130 34996966-5 2022 When rapamycin was combined with saturating concentrations of BafA, no significant further increase of LC3B lipidation could be detected over the levels induced by the late-stage inhibitor. Sirolimus 5-14 microtubule associated protein 1 light chain 3 beta Homo sapiens 103-107 34996966-3 2022 Here we scrutinize this approach and show that bafilomycin A1 (BafA) but not chloroquine is suitable for flux quantification due to the stimulating effect of chloroquine on non-canonical LC3B-lipidation. Chloroquine 158-169 microtubule associated protein 1 light chain 3 beta Homo sapiens 187-191 35014689-5 2022 Furthermore, it was found that forkhead box transcription factor O1 (FOXO1) enhanced PTX-induced autophagy through a transcriptional activation pattern in MDA-MB-231 cells, which was associated with the downstream target genes autophagy related 5, class III phosphoinositide 3-kinase vacuolar protein sorting 34, autophagy related 4B cysteine peptidase, beclin 1 and microtubule associated protein 1 light chain 3beta. Paclitaxel 85-88 microtubule associated protein 1 light chain 3 beta Homo sapiens 367-417 34996966-3 2022 Here we scrutinize this approach and show that bafilomycin A1 (BafA) but not chloroquine is suitable for flux quantification due to the stimulating effect of chloroquine on non-canonical LC3B-lipidation. bafilomycin A1 47-61 microtubule associated protein 1 light chain 3 beta Homo sapiens 187-191 34996966-3 2022 Here we scrutinize this approach and show that bafilomycin A1 (BafA) but not chloroquine is suitable for flux quantification due to the stimulating effect of chloroquine on non-canonical LC3B-lipidation. bafilomycin A1 63-67 microtubule associated protein 1 light chain 3 beta Homo sapiens 187-191 34996966-5 2022 When rapamycin was combined with saturating concentrations of BafA, no significant further increase of LC3B lipidation could be detected over the levels induced by the late-stage inhibitor. bafilomycin A1 62-66 microtubule associated protein 1 light chain 3 beta Homo sapiens 103-107 35154538-0 2022 Plumbagin triggers redox-mediated autophagy through the LC3B protein in human papillomavirus-positive cervical cancer cells. plumbagin 0-9 microtubule associated protein 1 light chain 3 beta Homo sapiens 56-60 35154538-7 2022 Translocation of LC-3B protein from the cytoplasm to the autophagosome was found in 31% of PL-treated cells, suggesting that PL provoked autophagic cell death. plumbagin 91-93 microtubule associated protein 1 light chain 3 beta Homo sapiens 17-22 35154538-7 2022 Translocation of LC-3B protein from the cytoplasm to the autophagosome was found in 31% of PL-treated cells, suggesting that PL provoked autophagic cell death. plumbagin 125-127 microtubule associated protein 1 light chain 3 beta Homo sapiens 17-22