PMID-sentid	Pub_year	Sent_text	comp_official_name	comp_offset	protein_name	organism	prot_offset
23934400-6	2014	All three of these TFs specifically bind the GCC box-like element of the GAG motif required for MeJA-induced transcription of NtPMT1a, a gene encoding putrescine N-methyltransferase, the first committed step in the synthesis of the nicotine pyrrolidine ring.	Glycosaminoglycans	73-76	putrescine N-methyltransferase 1	Nicotiana tabacum	151-181
23934400-6	2014	All three of these TFs specifically bind the GCC box-like element of the GAG motif required for MeJA-induced transcription of NtPMT1a, a gene encoding putrescine N-methyltransferase, the first committed step in the synthesis of the nicotine pyrrolidine ring.	nicotine pyrrolidine	232-252	putrescine N-methyltransferase 1	Nicotiana tabacum	151-181
21746701-5	2012	RNA interference (RNAi)-mediated knockdown of NtMYC2a and NtMYC2b expression results in significant decreases in JA-inducible NtPMT1a transcript levels, as well as reduced levels of transcripts encoding other enzymes involved in nicotine and minor alkaloid biosynthesis, including an 80-90% reduction in the level of transcripts encoding the putative nicotine synthase gene NtA662.	jasmonic acid	113-115	putrescine N-methyltransferase 1	Nicotiana tabacum	126-132
12453572-1	2002	Putrescine N-methyltransferase (PMT) catalyzes the first committed step in the biosynthesis of pyrrolinium ring-containing alkaloids.	pyrrolinium	95-106	putrescine N-methyltransferase 1	Nicotiana tabacum	0-30
18535352-2	2008	Several reports have described decreasing nicotine levels by silencing the putrescine N-methyltransferase (PMT) genes, but the reported variations of nicotine levels among transgenic lines are relatively low in general.	Nicotine	42-50	putrescine N-methyltransferase 1	Nicotiana tabacum	75-105
18535352-2	2008	Several reports have described decreasing nicotine levels by silencing the putrescine N-methyltransferase (PMT) genes, but the reported variations of nicotine levels among transgenic lines are relatively low in general.	Nicotine	42-50	putrescine N-methyltransferase 1	Nicotiana tabacum	107-110
18535352-2	2008	Several reports have described decreasing nicotine levels by silencing the putrescine N-methyltransferase (PMT) genes, but the reported variations of nicotine levels among transgenic lines are relatively low in general.	Nicotine	150-158	putrescine N-methyltransferase 1	Nicotiana tabacum	107-110
18535352-5	2008	By suppressing expression of the PMT genes, over 200 transgenic lines were obtained with nicotine levels reduced by 9.1-96.7%.	Nicotine	89-97	putrescine N-methyltransferase 1	Nicotiana tabacum	33-36
17004056-0	2007	Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent.	Tropanes	0-17	putrescine N-methyltransferase 1	Nicotiana tabacum	96-126
17004056-0	2007	Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent.	methyl jasmonate	130-146	putrescine N-methyltransferase 1	Nicotiana tabacum	96-126
17004056-1	2007	The cDNA from Nicotiana tabacum encoding Putrescine N-methyltransferase (PMT), which catalyzes the first committed step in the biosynthesis of tropane alkaloids, has been introduced into the genome of a scopolamine-producing Hyoscyamus niger mediated by the disarmed Agrobacterium tumefaciens strain C58C1, which also carries Agrobacterium rhizogenes Ri plasmid pRiA4, and expressed under the control of the CaMV 35S promoter.	Scopolamine	203-214	putrescine N-methyltransferase 1	Nicotiana tabacum	41-71
17004056-1	2007	The cDNA from Nicotiana tabacum encoding Putrescine N-methyltransferase (PMT), which catalyzes the first committed step in the biosynthesis of tropane alkaloids, has been introduced into the genome of a scopolamine-producing Hyoscyamus niger mediated by the disarmed Agrobacterium tumefaciens strain C58C1, which also carries Agrobacterium rhizogenes Ri plasmid pRiA4, and expressed under the control of the CaMV 35S promoter.	Scopolamine	203-214	putrescine N-methyltransferase 1	Nicotiana tabacum	73-76
17004056-2	2007	Hairy root lines transformed with pmt presented fivefold higher PMT activity than the control, and the methylputrescine (MPUT) levels of the resulting engineered hairy roots increased four to fivefold compared to the control and wild-type roots, but there was no significant increase in tropane alkaloids.	methylputrescine	103-119	putrescine N-methyltransferase 1	Nicotiana tabacum	34-37
17004056-2	2007	Hairy root lines transformed with pmt presented fivefold higher PMT activity than the control, and the methylputrescine (MPUT) levels of the resulting engineered hairy roots increased four to fivefold compared to the control and wild-type roots, but there was no significant increase in tropane alkaloids.	mput	121-125	putrescine N-methyltransferase 1	Nicotiana tabacum	34-37
15604714-1	2004	Putrescine N-methyltransferase (PMT) catalyzes the first committed step of nicotine biosynthesis, converting putrescine into N-methylputrescine.	Nicotine	75-83	putrescine N-methyltransferase 1	Nicotiana tabacum	0-30
15604714-1	2004	Putrescine N-methyltransferase (PMT) catalyzes the first committed step of nicotine biosynthesis, converting putrescine into N-methylputrescine.	Nicotine	75-83	putrescine N-methyltransferase 1	Nicotiana tabacum	32-35
15604714-1	2004	Putrescine N-methyltransferase (PMT) catalyzes the first committed step of nicotine biosynthesis, converting putrescine into N-methylputrescine.	Putrescine	109-119	putrescine N-methyltransferase 1	Nicotiana tabacum	0-30
15604714-1	2004	Putrescine N-methyltransferase (PMT) catalyzes the first committed step of nicotine biosynthesis, converting putrescine into N-methylputrescine.	Putrescine	109-119	putrescine N-methyltransferase 1	Nicotiana tabacum	32-35
15604714-1	2004	Putrescine N-methyltransferase (PMT) catalyzes the first committed step of nicotine biosynthesis, converting putrescine into N-methylputrescine.	N-Methylputrescine	125-143	putrescine N-methyltransferase 1	Nicotiana tabacum	0-30
15604714-1	2004	Putrescine N-methyltransferase (PMT) catalyzes the first committed step of nicotine biosynthesis, converting putrescine into N-methylputrescine.	N-Methylputrescine	125-143	putrescine N-methyltransferase 1	Nicotiana tabacum	32-35
20217418-4	2010	Putrescine N-methyltransferase (PMT) is a pathway-specific enzyme required in nicotinic alkaloid production in Nicotiana species.	nicotinic alkaloid	78-96	putrescine N-methyltransferase 1	Nicotiana tabacum	0-30
20217418-4	2010	Putrescine N-methyltransferase (PMT) is a pathway-specific enzyme required in nicotinic alkaloid production in Nicotiana species.	nicotinic alkaloid	78-96	putrescine N-methyltransferase 1	Nicotiana tabacum	32-35
20217418-9	2010	Under highly repressive conditions for alkaloid synthesis, including normal culture conditions in the light, both ROS scavengers resulted in significant induction of PMT promoter activity.	Reactive Oxygen Species	114-117	putrescine N-methyltransferase 1	Nicotiana tabacum	166-169
17004056-4	2007	The results indicate that hairy root lines over-expressing pmt have a high capacity to synthesize MPUT, whereas their ability to convert hyoscyamine into scopolamine is very limited.	Hyoscyamine	137-148	putrescine N-methyltransferase 1	Nicotiana tabacum	59-62
14756309-0	2003	Antisense-mediated down-regulation of putrescine N-methyltransferase activity in transgenic Nicotiana tabacum L. can lead to elevated levels of anatabine at the expense of nicotine.	anatabine	144-153	putrescine N-methyltransferase 1	Nicotiana tabacum	38-68
14756309-0	2003	Antisense-mediated down-regulation of putrescine N-methyltransferase activity in transgenic Nicotiana tabacum L. can lead to elevated levels of anatabine at the expense of nicotine.	Nicotine	172-180	putrescine N-methyltransferase 1	Nicotiana tabacum	38-68
14756309-5	2003	Several antisense-PMT transformed root lines, and also leaf tissues of regenerated transformed plants, showed a substantial increase in anatabine content relative to controls.	anatabine	136-145	putrescine N-methyltransferase 1	Nicotiana tabacum	18-21
14756309-8	2003	We suggest that elevated anatabine levels in antisense-PMT lines are a direct consequence of a relative oversupply of nicotinic acid which, in the absence of adequate levels of 1-methyl-delta(1)-pyrrolinium cation (the ultimate product of PMT activity), is used to synthesise anatabine directly.	anatabine	25-34	putrescine N-methyltransferase 1	Nicotiana tabacum	55-58
14756309-8	2003	We suggest that elevated anatabine levels in antisense-PMT lines are a direct consequence of a relative oversupply of nicotinic acid which, in the absence of adequate levels of 1-methyl-delta(1)-pyrrolinium cation (the ultimate product of PMT activity), is used to synthesise anatabine directly.	anatabine	25-34	putrescine N-methyltransferase 1	Nicotiana tabacum	239-242
14756309-8	2003	We suggest that elevated anatabine levels in antisense-PMT lines are a direct consequence of a relative oversupply of nicotinic acid which, in the absence of adequate levels of 1-methyl-delta(1)-pyrrolinium cation (the ultimate product of PMT activity), is used to synthesise anatabine directly.	Niacin	118-132	putrescine N-methyltransferase 1	Nicotiana tabacum	55-58
14756309-8	2003	We suggest that elevated anatabine levels in antisense-PMT lines are a direct consequence of a relative oversupply of nicotinic acid which, in the absence of adequate levels of 1-methyl-delta(1)-pyrrolinium cation (the ultimate product of PMT activity), is used to synthesise anatabine directly.	Niacin	118-132	putrescine N-methyltransferase 1	Nicotiana tabacum	239-242
14756309-8	2003	We suggest that elevated anatabine levels in antisense-PMT lines are a direct consequence of a relative oversupply of nicotinic acid which, in the absence of adequate levels of 1-methyl-delta(1)-pyrrolinium cation (the ultimate product of PMT activity), is used to synthesise anatabine directly.	anatabine	276-285	putrescine N-methyltransferase 1	Nicotiana tabacum	55-58
14756309-9	2003	As is discussed, no naturally occurring species or varieties of Nicotiana are known that typically produce high levels of anatabine in root or leaf tissues, meaning that the antisense PMT transgenics produced in this study have no natural counterpart.	anatabine	122-131	putrescine N-methyltransferase 1	Nicotiana tabacum	184-187
12453572-1	2002	Putrescine N-methyltransferase (PMT) catalyzes the first committed step in the biosynthesis of pyrrolinium ring-containing alkaloids.	pyrrolinium	95-106	putrescine N-methyltransferase 1	Nicotiana tabacum	32-35
12453572-1	2002	Putrescine N-methyltransferase (PMT) catalyzes the first committed step in the biosynthesis of pyrrolinium ring-containing alkaloids.	-containing alkaloids	111-132	putrescine N-methyltransferase 1	Nicotiana tabacum	0-30
12453572-1	2002	Putrescine N-methyltransferase (PMT) catalyzes the first committed step in the biosynthesis of pyrrolinium ring-containing alkaloids.	-containing alkaloids	111-132	putrescine N-methyltransferase 1	Nicotiana tabacum	32-35
11198422-5	2000	QPRTase transcript levels increased markedly in roots of both species 12-24 h after damage to aerial tissues, with a concomitant rise in transcript levels of putrescine N-methyltransferase (PMT), another key enzyme in nicotine biosynthesis.	Nicotine	218-226	putrescine N-methyltransferase 1	Nicotiana tabacum	158-188
12369619-6	2002	The A622 expression patterns were qualitatively similar to those of putrescine N-methyltransferase, the first enzyme in nicotine biosynthesis, suggesting that A622 may function in the metabolism of nicotine or related alkaloids.	Nicotine	120-128	putrescine N-methyltransferase 1	Nicotiana tabacum	68-98
12369619-6	2002	The A622 expression patterns were qualitatively similar to those of putrescine N-methyltransferase, the first enzyme in nicotine biosynthesis, suggesting that A622 may function in the metabolism of nicotine or related alkaloids.	Nicotine	198-206	putrescine N-methyltransferase 1	Nicotiana tabacum	68-98
12369619-6	2002	The A622 expression patterns were qualitatively similar to those of putrescine N-methyltransferase, the first enzyme in nicotine biosynthesis, suggesting that A622 may function in the metabolism of nicotine or related alkaloids.	Alkaloids	218-227	putrescine N-methyltransferase 1	Nicotiana tabacum	68-98
11198422-5	2000	QPRTase transcript levels increased markedly in roots of both species 12-24 h after damage to aerial tissues, with a concomitant rise in transcript levels of putrescine N-methyltransferase (PMT), another key enzyme in nicotine biosynthesis.	Nicotine	218-226	putrescine N-methyltransferase 1	Nicotiana tabacum	190-193
10598105-1	1999	The structure and nuclear genomic organization of the gene family encoding putrescine N-methyltransferase (PMT), the key enzyme in diverting polyamine metabolism towards the biosynthesis of nicotine and related alkaloids, was examined in Nicotiana tabacum.	Polyamines	141-150	putrescine N-methyltransferase 1	Nicotiana tabacum	75-105
10598105-1	1999	The structure and nuclear genomic organization of the gene family encoding putrescine N-methyltransferase (PMT), the key enzyme in diverting polyamine metabolism towards the biosynthesis of nicotine and related alkaloids, was examined in Nicotiana tabacum.	Polyamines	141-150	putrescine N-methyltransferase 1	Nicotiana tabacum	107-110
10598105-1	1999	The structure and nuclear genomic organization of the gene family encoding putrescine N-methyltransferase (PMT), the key enzyme in diverting polyamine metabolism towards the biosynthesis of nicotine and related alkaloids, was examined in Nicotiana tabacum.	Nicotine	190-198	putrescine N-methyltransferase 1	Nicotiana tabacum	75-105
10598105-1	1999	The structure and nuclear genomic organization of the gene family encoding putrescine N-methyltransferase (PMT), the key enzyme in diverting polyamine metabolism towards the biosynthesis of nicotine and related alkaloids, was examined in Nicotiana tabacum.	Nicotine	190-198	putrescine N-methyltransferase 1	Nicotiana tabacum	107-110
29379516-3	2017	Here we isolated a transcription factor NtWRKY-R1 from the group IIe of WRKY family and it had strong negative correlation with the expression of putrescine N-methyltransferase, the key enzyme of nicotine synthesis pathway.	Nicotine	196-204	putrescine N-methyltransferase 1	Nicotiana tabacum	146-176
16660940-3	1979	Putrescine N-methyltransferase (EC 2.1.1.53) and quinolinic acid phosphoribosyltransferase (EC 2.4.2.19) activities in root tissue of these four genotypes were proportional to leaf nicotine level, whereas N-methylputrescine oxidase activity in root tissue differed in proportion and ranking.	Nicotine	181-189	putrescine N-methyltransferase 1	Nicotiana tabacum	0-30
29316708-6	2018	CO causes an increased expression of NtPMT1 (a key nicotine biosynthesis enzyme), via promoting NtMYC2a binding to the G-box region of its promoter, leading to heightened nicotine levels under HT conditions.	Nicotine	51-59	putrescine N-methyltransferase 1	Nicotiana tabacum	37-43
29316708-6	2018	CO causes an increased expression of NtPMT1 (a key nicotine biosynthesis enzyme), via promoting NtMYC2a binding to the G-box region of its promoter, leading to heightened nicotine levels under HT conditions.	Nicotine	171-179	putrescine N-methyltransferase 1	Nicotiana tabacum	37-43
27833561-1	2016	Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation.	jasmonic acid	43-56	putrescine N-methyltransferase 1	Nicotiana tabacum	205-237
27833561-1	2016	Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation.	jasmonic acid	58-60	putrescine N-methyltransferase 1	Nicotiana tabacum	205-237
27833561-1	2016	Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation.	jasmonic acid	58-60	putrescine N-methyltransferase 1	Nicotiana tabacum	239-245
27833561-1	2016	Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation.	Nicotine	96-104	putrescine N-methyltransferase 1	Nicotiana tabacum	205-237
27833561-1	2016	Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation.	Nicotine	96-104	putrescine N-methyltransferase 1	Nicotiana tabacum	239-245
27833561-1	2016	Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation.	pyridine alkaloids	117-135	putrescine N-methyltransferase 1	Nicotiana tabacum	205-237
27833561-1	2016	Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation.	pyridine alkaloids	117-135	putrescine N-methyltransferase 1	Nicotiana tabacum	239-245
27833561-1	2016	Environmental stress elevates the level of jasmonic acid (JA) and activates the biosynthesis of nicotine and related pyridine alkaloids in tobacco (Nicotiana tabacum L.) by up-regulating the expression of putrescine N-methyltransferase 1 (NtPMT1), which encodes a putrescine N-methyl transferase that catalyzes nicotine formation.	Nicotine	311-319	putrescine N-methyltransferase 1	Nicotiana tabacum	205-237
27833561-4	2016	Overexpression of NtMYC2a increased nicotine biosynthesis by enhancing its binding to the promoter of NtPMT1.	Nicotine	36-44	putrescine N-methyltransferase 1	Nicotiana tabacum	102-108