PMID-sentid Pub_year Sent_text comp_official_name comp_offset protein_name organism prot_offset 11415453-4 2001 Mutation of the active-site residues Asp(88) or His(118) within the human PP2A catalytic subunit (PP2Ac)alpha impaired catalytic activity in vitro; the D88N and H118N substitutions caused a 9- and 23-fold reduction in specific activity respectively, when compared with wild-type recombinant PP2Ac, indicating an important role for these residues in catalysis. Aspartic Acid 37-40 protein phosphatase 2 catalytic subunit alpha Homo sapiens 98-103 11415453-4 2001 Mutation of the active-site residues Asp(88) or His(118) within the human PP2A catalytic subunit (PP2Ac)alpha impaired catalytic activity in vitro; the D88N and H118N substitutions caused a 9- and 23-fold reduction in specific activity respectively, when compared with wild-type recombinant PP2Ac, indicating an important role for these residues in catalysis. Histidine 48-51 protein phosphatase 2 catalytic subunit alpha Homo sapiens 98-103 11440182-13 2001 Another general observation was that the complexes with Mn dications were more stable than those with Zn dications for both PP1c and PP2Ac units. Zinc 102-104 protein phosphatase 2 catalytic subunit alpha Homo sapiens 133-138 9030574-11 1997 PP-1c and PP-2Ac are also targets for other marine toxins such as okadaic acid and calyculin A. Okadaic Acid 66-78 protein phosphatase 2 catalytic subunit alpha Homo sapiens 10-16 11007961-5 2000 Other molecular mechanisms that regulate PP2Ac function include phosphorylation, carboxyl methylation, inhibition by intracellular protein inhibitors (I(1)(PP2A) and I(2)(PP2A)), and stimulation by ceramide. Ceramides 198-206 protein phosphatase 2 catalytic subunit alpha Homo sapiens 41-46 10318862-4 1999 Here, we report that substitution of glutamine for either of two putative active site histidines in the PP2A C subunit results in inactivation of PP2A and formation of stable complexes between PP2A and several cellular proteins. Glutamine 37-46 protein phosphatase 2 catalytic subunit alpha Homo sapiens 104-110 10318862-4 1999 Here, we report that substitution of glutamine for either of two putative active site histidines in the PP2A C subunit results in inactivation of PP2A and formation of stable complexes between PP2A and several cellular proteins. Histidine 86-96 protein phosphatase 2 catalytic subunit alpha Homo sapiens 104-110 9877231-1 1998 Recently, we demonstrated that the 36 kDa catalytic subunit of protein phosphatase 2A (PP2Ac) undergoes methylation at its C-terminal leucine in normal rat islets, human islets and isolated beta cells; this modification increases the catalytic activity of PP2A [Kowluru et al. Leucine 134-141 protein phosphatase 2 catalytic subunit alpha Homo sapiens 87-92 9877231-6 1998 Micromolar concentrations of ATP, ADP, GTP or GDP each inhibited the carboxyl methylation of PP2Ac and, to a lesser degree, the catalytic activity of PP2A. Adenosine Triphosphate 29-32 protein phosphatase 2 catalytic subunit alpha Homo sapiens 93-98 9877231-6 1998 Micromolar concentrations of ATP, ADP, GTP or GDP each inhibited the carboxyl methylation of PP2Ac and, to a lesser degree, the catalytic activity of PP2A. Adenosine Diphosphate 34-37 protein phosphatase 2 catalytic subunit alpha Homo sapiens 93-98 9877231-6 1998 Micromolar concentrations of ATP, ADP, GTP or GDP each inhibited the carboxyl methylation of PP2Ac and, to a lesser degree, the catalytic activity of PP2A. Guanosine Triphosphate 39-42 protein phosphatase 2 catalytic subunit alpha Homo sapiens 93-98 9877231-6 1998 Micromolar concentrations of ATP, ADP, GTP or GDP each inhibited the carboxyl methylation of PP2Ac and, to a lesser degree, the catalytic activity of PP2A. Guanosine Diphosphate 46-49 protein phosphatase 2 catalytic subunit alpha Homo sapiens 93-98 9877231-7 1998 Likewise, the carboxyl methylation of PP2Ac and its catalytic activity were inhibited by [mono- or di-] phosphates of glucose or fructose. [mono- or di-] phosphates 89-114 protein phosphatase 2 catalytic subunit alpha Homo sapiens 38-43 9877231-7 1998 Likewise, the carboxyl methylation of PP2Ac and its catalytic activity were inhibited by [mono- or di-] phosphates of glucose or fructose. Glucose 118-125 protein phosphatase 2 catalytic subunit alpha Homo sapiens 38-43 9877231-7 1998 Likewise, the carboxyl methylation of PP2Ac and its catalytic activity were inhibited by [mono- or di-] phosphates of glucose or fructose. Fructose 129-137 protein phosphatase 2 catalytic subunit alpha Homo sapiens 38-43 9877231-8 1998 Additionally, however, the carboxyl methylation of PP2Ac was significantly stimulated by divalent metal ions (Mn2+ > Mg2+ > Ca2+ > control). Metals 98-103 protein phosphatase 2 catalytic subunit alpha Homo sapiens 51-56 9877231-8 1998 Additionally, however, the carboxyl methylation of PP2Ac was significantly stimulated by divalent metal ions (Mn2+ > Mg2+ > Ca2+ > control). Manganese(2+) 110-114 protein phosphatase 2 catalytic subunit alpha Homo sapiens 51-56 9877231-8 1998 Additionally, however, the carboxyl methylation of PP2Ac was significantly stimulated by divalent metal ions (Mn2+ > Mg2+ > Ca2+ > control). magnesium ion 120-124 protein phosphatase 2 catalytic subunit alpha Homo sapiens 51-56 9877231-9 1998 The nucleotide or sugar phosphate-mediated inhibition of carboxyl methylation of PP2Ac and the catalytic activity of PP2A were completely prevented by Mn2+ or Mg2+. Sugar Phosphates 18-33 protein phosphatase 2 catalytic subunit alpha Homo sapiens 81-86 9877231-9 1998 The nucleotide or sugar phosphate-mediated inhibition of carboxyl methylation of PP2Ac and the catalytic activity of PP2A were completely prevented by Mn2+ or Mg2+. Manganese(2+) 151-155 protein phosphatase 2 catalytic subunit alpha Homo sapiens 81-86 9877231-9 1998 The nucleotide or sugar phosphate-mediated inhibition of carboxyl methylation of PP2Ac and the catalytic activity of PP2A were completely prevented by Mn2+ or Mg2+. magnesium ion 159-163 protein phosphatase 2 catalytic subunit alpha Homo sapiens 81-86 9877231-10 1998 These data indicate that divalent metal ions protect against the inhibition by purine nucleotides or sugar phosphates of the carboxyl methylation of PP2Ac perhaps permitting PP2A to function under physiologic conditions. Metals 34-39 protein phosphatase 2 catalytic subunit alpha Homo sapiens 149-154 9877231-10 1998 These data indicate that divalent metal ions protect against the inhibition by purine nucleotides or sugar phosphates of the carboxyl methylation of PP2Ac perhaps permitting PP2A to function under physiologic conditions. Purine Nucleotides 79-97 protein phosphatase 2 catalytic subunit alpha Homo sapiens 149-154 9877231-10 1998 These data indicate that divalent metal ions protect against the inhibition by purine nucleotides or sugar phosphates of the carboxyl methylation of PP2Ac perhaps permitting PP2A to function under physiologic conditions. Sugar Phosphates 101-117 protein phosphatase 2 catalytic subunit alpha Homo sapiens 149-154 9657754-4 1998 Rapamycin treatment disrupts the association of PP2Ac/alpha4 in parallel with the inhibitory effect of lymphoid cell proliferation. Sirolimus 0-9 protein phosphatase 2 catalytic subunit alpha Homo sapiens 48-53 9657754-7 1998 Introduction of the malpha4 cDNA into Jurkat cells or the increased association of PP2Ac/alpha4 by the culture with low serum concentration confers cells with rapamycin resistance. Sirolimus 159-168 protein phosphatase 2 catalytic subunit alpha Homo sapiens 83-88 11027216-8 2000 Whole-cell treatments with okadaic acid or calyculin A diminished SERT/PP2Ac associations. Okadaic Acid 27-39 protein phosphatase 2 catalytic subunit alpha Homo sapiens 71-76 11027216-9 2000 Phorbol esters, which trigger SERT phosphorylation, also diminish SERT/PP2Ac associations, effects that can be blocked by PKC antagonists as well as the SERT substrate 5-HT. Phorbol Esters 0-14 protein phosphatase 2 catalytic subunit alpha Homo sapiens 71-76 10191253-1 1999 Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro, but the functional consequence(s) of this post-translational modification in the context of the cell remain unclear. Leucine 30-37 protein phosphatase 2 catalytic subunit alpha Homo sapiens 83-115 10191253-1 1999 Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro, but the functional consequence(s) of this post-translational modification in the context of the cell remain unclear. Leucine 30-37 protein phosphatase 2 catalytic subunit alpha Homo sapiens 117-122 10191253-2 1999 Alkali-induced demethylation of PP2AC in purified PP2A heterotrimer (ABalphaC), but not in purified PP2A heterodimer (AC), indicated that a larger fraction of PP2AC is carboxymethylated in ABalphaC than in AC. abalphac 69-77 protein phosphatase 2 catalytic subunit alpha Homo sapiens 32-37 10191253-2 1999 Alkali-induced demethylation of PP2AC in purified PP2A heterotrimer (ABalphaC), but not in purified PP2A heterodimer (AC), indicated that a larger fraction of PP2AC is carboxymethylated in ABalphaC than in AC. abalphac 69-77 protein phosphatase 2 catalytic subunit alpha Homo sapiens 159-164 10191253-8 1999 These results demonstrate that the reversibly methylated C-terminal leucine residue of PP2AC is important for Balpha regulatory subunit binding. Leucine 68-75 protein phosphatase 2 catalytic subunit alpha Homo sapiens 87-92 9170161-10 1997 First, the c-AMP inhibitor, 10(-3) M Rp-c-AMPS, completely inhibited the stimulatory effect of alpha-MSH in these in vitro melanocytes. adenosine 5'-phosphorothioate 42-46 protein phosphatase 2 catalytic subunit alpha Homo sapiens 37-41 8910475-3 1996 Subsequent anion-exchange chromatography and SDS-polyacrylamide gel electrophoresis of the microcystin-biotin eluates of the three fractions revealed a complex pattern of proteins associated with PP-1C and PP-2AC. microcystin-biotin 91-109 protein phosphatase 2 catalytic subunit alpha Homo sapiens 206-212 8960363-6 1996 Specific reduction of microcystin-LA to dihydromicrocystin-LA abolished the ability of the toxin to form a covalent adduct with PP-2Ac. cyanoginosin-LA 22-36 protein phosphatase 2 catalytic subunit alpha Homo sapiens 128-134 8910475-2 1996 Biotinylated microcystin displayed IC50 values in the nM range against PP-1C (1.58 +/- 0.6 nM S.E., n = 3), PP-2AC (0.63 +/- 0.2 nM S.E., n = 3) and SMPP-1M (5.9 +/- 1.3 S.E., n = 3). microcystin 13-24 protein phosphatase 2 catalytic subunit alpha Homo sapiens 108-114 8950780-1 1996 Charge-to-alanine mutations of three amino acid residues, viz, D46, D48, and D/Hya71, which are known to be important in stabilizing Ca2+ binding to epidermal growth factor (EGF) domains of vitamin K-dependent blood coagulation proteins, have been engineered into recombinant human protein C (r-PC). Alanine 10-17 protein phosphatase 2 catalytic subunit alpha Homo sapiens 293-297 8950780-1 1996 Charge-to-alanine mutations of three amino acid residues, viz, D46, D48, and D/Hya71, which are known to be important in stabilizing Ca2+ binding to epidermal growth factor (EGF) domains of vitamin K-dependent blood coagulation proteins, have been engineered into recombinant human protein C (r-PC). Vitamin K 190-199 protein phosphatase 2 catalytic subunit alpha Homo sapiens 293-297 8950780-6 1996 All of the mutants were reactive against a monoclonal antibody (MAb) specific for a Ca(2+)-dependent epitope within the amino-terminus of the Gla domain of r-PC, demonstrating that a proper Ca(2+)-dependent conformation was adopted in this region of the protein. 1-Carboxyglutamic Acid 142-145 protein phosphatase 2 catalytic subunit alpha Homo sapiens 156-160 8809040-2 1996 Okadaic acid, an inhibitor of phosphoprotein phosphatases type 1 (PP1) and 2A (PP2A), increased the probability of channels being in the open state (Po) in intact cells. Okadaic Acid 0-12 protein phosphatase 2 catalytic subunit alpha Homo sapiens 79-83 8809040-6 1996 This effect of PP2Ac was completely prevented by 1 microM okadaic acid. Okadaic Acid 58-70 protein phosphatase 2 catalytic subunit alpha Homo sapiens 15-20 8950780-8 1996 These data strongly suggest that the alanine mutations present at D46, D48, and D71 diminished Ca2+ binding to the EGF1 domain of r-PC. Alanine 37-44 protein phosphatase 2 catalytic subunit alpha Homo sapiens 130-134 8960363-9 1996 In contrast, following rapid inactivation of PP-2Ac/PP-1c by motuporin, the equivalent N-methyldehydrobutyrine residue in this toxin is unreactive and does not form a covalent bond with the PPases. N-Methyldehydrobutyrine 87-110 protein phosphatase 2 catalytic subunit alpha Homo sapiens 45-51 8960363-6 1996 Specific reduction of microcystin-LA to dihydromicrocystin-LA abolished the ability of the toxin to form a covalent adduct with PP-2Ac. dihydromicrocystin-la 40-61 protein phosphatase 2 catalytic subunit alpha Homo sapiens 128-134 8960363-8 1996 Our data indicate that inactivation of PP-2Ac/PP-1c by microcystins precedes covalent modification of the PPases via a Michael addition reaction between a nucleophilic phosphatase residue and Mdha in the heptapeptide toxin. Microcystins 55-67 protein phosphatase 2 catalytic subunit alpha Homo sapiens 39-45 8960363-9 1996 In contrast, following rapid inactivation of PP-2Ac/PP-1c by motuporin, the equivalent N-methyldehydrobutyrine residue in this toxin is unreactive and does not form a covalent bond with the PPases. motuporin 61-70 protein phosphatase 2 catalytic subunit alpha Homo sapiens 45-51 8069853-4 1994 Western blot analysis showed that the level of PP2A catalytic subunit (PP2A-C) decreased during the course of ATRA-induced differentiation, whereas expressions of A and B (M(r) 55,000) regulatory subunits of PP2A were relatively unaltered. Tretinoin 110-114 protein phosphatase 2 catalytic subunit alpha Homo sapiens 47-69 8549768-3 1995 AMP inhibited the dephosphorylation of AMP-activated protein kinase (AMPK), but not phosphocasein, by PP2C alpha. Adenosine Monophosphate 0-3 protein phosphatase 2 catalytic subunit alpha Homo sapiens 102-112 8529028-1 1995 The catalytic subunit of protein phosphatase 2A (PP2Ac) was purified from Neurospora crassa extract by (NH4)2SO4-ethanol precipitation followed by DEAE-Sephacel, heparin-Sepharose, and MonoQ chromatography steps about 900-fold to a specific activity of 1200 U/g with a 2% yield. (nh4)2so4-ethanol 103-120 protein phosphatase 2 catalytic subunit alpha Homo sapiens 49-54 8529028-1 1995 The catalytic subunit of protein phosphatase 2A (PP2Ac) was purified from Neurospora crassa extract by (NH4)2SO4-ethanol precipitation followed by DEAE-Sephacel, heparin-Sepharose, and MonoQ chromatography steps about 900-fold to a specific activity of 1200 U/g with a 2% yield. deae-sephacel 147-160 protein phosphatase 2 catalytic subunit alpha Homo sapiens 49-54 8529028-1 1995 The catalytic subunit of protein phosphatase 2A (PP2Ac) was purified from Neurospora crassa extract by (NH4)2SO4-ethanol precipitation followed by DEAE-Sephacel, heparin-Sepharose, and MonoQ chromatography steps about 900-fold to a specific activity of 1200 U/g with a 2% yield. Heparin 162-169 protein phosphatase 2 catalytic subunit alpha Homo sapiens 49-54 8529028-1 1995 The catalytic subunit of protein phosphatase 2A (PP2Ac) was purified from Neurospora crassa extract by (NH4)2SO4-ethanol precipitation followed by DEAE-Sephacel, heparin-Sepharose, and MonoQ chromatography steps about 900-fold to a specific activity of 1200 U/g with a 2% yield. Sepharose 170-179 protein phosphatase 2 catalytic subunit alpha Homo sapiens 49-54 8529028-2 1995 The apparent M(r) of PP2Ac was estimated to be 35 kDa by gel filtration and 33 kDa by SDS polyacrylamide gel electrophoresis. Sodium Dodecyl Sulfate 86-89 protein phosphatase 2 catalytic subunit alpha Homo sapiens 21-26 8529028-2 1995 The apparent M(r) of PP2Ac was estimated to be 35 kDa by gel filtration and 33 kDa by SDS polyacrylamide gel electrophoresis. polyacrylamide 90-104 protein phosphatase 2 catalytic subunit alpha Homo sapiens 21-26 8529028-3 1995 Half maximal inhibition of PP2Ac was achieved at 0.3 nM okadaic acid, 0.1 nM microcystin-LR, 56 nM cantharidin and 280 nM endothall concentrations. Okadaic Acid 56-68 protein phosphatase 2 catalytic subunit alpha Homo sapiens 27-32 8529028-3 1995 Half maximal inhibition of PP2Ac was achieved at 0.3 nM okadaic acid, 0.1 nM microcystin-LR, 56 nM cantharidin and 280 nM endothall concentrations. cyanoginosin LR 77-91 protein phosphatase 2 catalytic subunit alpha Homo sapiens 27-32 8529028-3 1995 Half maximal inhibition of PP2Ac was achieved at 0.3 nM okadaic acid, 0.1 nM microcystin-LR, 56 nM cantharidin and 280 nM endothall concentrations. Cantharidin 99-110 protein phosphatase 2 catalytic subunit alpha Homo sapiens 27-32 8069853-4 1994 Western blot analysis showed that the level of PP2A catalytic subunit (PP2A-C) decreased during the course of ATRA-induced differentiation, whereas expressions of A and B (M(r) 55,000) regulatory subunits of PP2A were relatively unaltered. Tretinoin 110-114 protein phosphatase 2 catalytic subunit alpha Homo sapiens 71-77 8069853-6 1994 Northern blot analysis revealed that mRNA levels of PP2A-C beta and A alpha regulatory subunits were decreased following treatment with ATRA, while levels of PP2A-C alpha and B (M(r) 55,000) alpha regulatory subunit transcripts were relatively constant. Tretinoin 136-140 protein phosphatase 2 catalytic subunit alpha Homo sapiens 52-58 33912173-0 2021 Taurine Antagonizes Macrophages M1 Polarization by Mitophagy-Glycolysis Switch Blockage via Dragging SAM-PP2Ac Transmethylation. Taurine 0-7 protein phosphatase 2 catalytic subunit alpha Homo sapiens 105-110 34922339-0 2022 The miR-345-3p/PPP2CA signaling axis promotes proliferation and invasion of breast cancer cells. mir-345-3p 4-14 protein phosphatase 2 catalytic subunit alpha Homo sapiens 15-21 34922339-7 2022 Blocking miR-345-3p relieves its inhibition of PPP2CA, which attenuated PI3K-AKT signaling pathway. mir-345-3p 9-19 protein phosphatase 2 catalytic subunit alpha Homo sapiens 47-53 34998390-8 2022 In resting cells, an IGBP1-PP2Ac dimer binds to PI3K, dephosphorylates the inhibitory pSer608-p85 of PI3K and thus maintains its high basal activity. pser608 86-93 protein phosphatase 2 catalytic subunit alpha Homo sapiens 27-32 8392031-4 1993 From these studies, PP-2A alpha has been thought to be involved in proliferation, and we expected that PP-2A alpha would decrease in HL-60 cells on dimethyl sulfoxide(DMSO)-induced granulocytic differentiation with decreased growth rate. Dimethyl Sulfoxide 148-166 protein phosphatase 2 catalytic subunit alpha Homo sapiens 103-114 8392031-4 1993 From these studies, PP-2A alpha has been thought to be involved in proliferation, and we expected that PP-2A alpha would decrease in HL-60 cells on dimethyl sulfoxide(DMSO)-induced granulocytic differentiation with decreased growth rate. Dimethyl Sulfoxide 167-171 protein phosphatase 2 catalytic subunit alpha Homo sapiens 20-31 8392031-4 1993 From these studies, PP-2A alpha has been thought to be involved in proliferation, and we expected that PP-2A alpha would decrease in HL-60 cells on dimethyl sulfoxide(DMSO)-induced granulocytic differentiation with decreased growth rate. Dimethyl Sulfoxide 167-171 protein phosphatase 2 catalytic subunit alpha Homo sapiens 103-114 8392031-5 1993 In this study, we show that PP-2A alpha expression increases in HL-60 cells on DMSO-induced granulocytic differentiation, while PP-1 gamma, which is one of catalytic subunits of protein phosphatase 1, expression did not change significantly. Dimethyl Sulfoxide 79-83 protein phosphatase 2 catalytic subunit alpha Homo sapiens 28-39 34536862-8 2022 On the contrary, the soil recalcitrant carbon (RP-C) did not vary much across the wetland soils. Carbon 39-45 protein phosphatase 2 catalytic subunit alpha Homo sapiens 47-51 33991460-7 2021 Increasing the levels of cellular polyamines by stable transfection with the Odc gene stimulated alpha4 and PP2Ac expression and enhanced their association, thus also promoting epithelial restitution after wounding. Polyamines 34-44 protein phosphatase 2 catalytic subunit alpha Homo sapiens 108-113 33991460-8 2021 In contrast, depletion of cellular polyamines by treatment with alpha-difluoromethylornithine reduced alpha4/PP2Ac complexes and repressed cell migration. Polyamines 35-45 protein phosphatase 2 catalytic subunit alpha Homo sapiens 109-114 33991460-8 2021 In contrast, depletion of cellular polyamines by treatment with alpha-difluoromethylornithine reduced alpha4/PP2Ac complexes and repressed cell migration. Eflornithine 64-93 protein phosphatase 2 catalytic subunit alpha Homo sapiens 109-114 33912173-0 2021 Taurine Antagonizes Macrophages M1 Polarization by Mitophagy-Glycolysis Switch Blockage via Dragging SAM-PP2Ac Transmethylation. S-Adenosylmethionine 101-104 protein phosphatase 2 catalytic subunit alpha Homo sapiens 105-110 33912173-11 2021 Mechanistically, taurine inhibits SAM-dependent PP2Ac methylation to block PINK1-mediated mitophagy flux, thereby maintaining a high mitochondrial density, which ultimately hinders the conversion of energy metabolism to glycolysis required for M1. Taurine 17-24 protein phosphatase 2 catalytic subunit alpha Homo sapiens 48-53 32616123-4 2020 Conclusion Down-regulation of PP2Ac is involved in the abnormal shape and distribution of mitochondria and its dysfunction induced by human tau40 in rat primary hippocampal neurons and HEK293 cells. tau40 140-145 protein phosphatase 2 catalytic subunit alpha Homo sapiens 30-35 32663263-4 2020 Protein phosphatase 2A (PP2A) is an essential serine/threonine protein phosphatase that regulates a broad range of cellular signaling. Serine 46-52 protein phosphatase 2 catalytic subunit alpha Homo sapiens 24-28 33000885-10 2020 Taken together, our results suggest that PP2Ac nitration during cAMP-induced decidualization of hESCs is induced through the Epac1-Rap1-PLCepsilon-CaMKII-HDAC5-iNOS signaling pathway. Cyclic AMP 64-68 protein phosphatase 2 catalytic subunit alpha Homo sapiens 41-46 32492475-0 2020 Reduced methylation of PP2Ac promotes ethanol-induced lipid accumulation through FOXO1 phosphorylation in vitro and in vivo. Ethanol 38-45 protein phosphatase 2 catalytic subunit alpha Homo sapiens 23-28 32492475-3 2020 We found that the severity of ethanol-induced liver injury and the extent of demethylation of PP2A catalytic C subunit (PP2Ac) were reduced after treatment with betaine, a methyl donor involved in the methionine-homocysteine cycle. Ethanol 30-37 protein phosphatase 2 catalytic subunit alpha Homo sapiens 94-118 32492475-3 2020 We found that the severity of ethanol-induced liver injury and the extent of demethylation of PP2A catalytic C subunit (PP2Ac) were reduced after treatment with betaine, a methyl donor involved in the methionine-homocysteine cycle. Ethanol 30-37 protein phosphatase 2 catalytic subunit alpha Homo sapiens 120-125 32492475-3 2020 We found that the severity of ethanol-induced liver injury and the extent of demethylation of PP2A catalytic C subunit (PP2Ac) were reduced after treatment with betaine, a methyl donor involved in the methionine-homocysteine cycle. Betaine 161-168 protein phosphatase 2 catalytic subunit alpha Homo sapiens 94-118 32492475-3 2020 We found that the severity of ethanol-induced liver injury and the extent of demethylation of PP2A catalytic C subunit (PP2Ac) were reduced after treatment with betaine, a methyl donor involved in the methionine-homocysteine cycle. Betaine 161-168 protein phosphatase 2 catalytic subunit alpha Homo sapiens 120-125 32492475-3 2020 We found that the severity of ethanol-induced liver injury and the extent of demethylation of PP2A catalytic C subunit (PP2Ac) were reduced after treatment with betaine, a methyl donor involved in the methionine-homocysteine cycle. Methionine 201-211 protein phosphatase 2 catalytic subunit alpha Homo sapiens 94-118 32492475-3 2020 We found that the severity of ethanol-induced liver injury and the extent of demethylation of PP2A catalytic C subunit (PP2Ac) were reduced after treatment with betaine, a methyl donor involved in the methionine-homocysteine cycle. Methionine 201-211 protein phosphatase 2 catalytic subunit alpha Homo sapiens 120-125 32492475-3 2020 We found that the severity of ethanol-induced liver injury and the extent of demethylation of PP2A catalytic C subunit (PP2Ac) were reduced after treatment with betaine, a methyl donor involved in the methionine-homocysteine cycle. Homocysteine 212-224 protein phosphatase 2 catalytic subunit alpha Homo sapiens 94-118 32492475-3 2020 We found that the severity of ethanol-induced liver injury and the extent of demethylation of PP2A catalytic C subunit (PP2Ac) were reduced after treatment with betaine, a methyl donor involved in the methionine-homocysteine cycle. Homocysteine 212-224 protein phosphatase 2 catalytic subunit alpha Homo sapiens 120-125 32492475-4 2020 These results suggest that PP2Ac methylation is decreased due to a broad decrease in hepatic methylation capacity after exposure to ethanol. Ethanol 132-139 protein phosphatase 2 catalytic subunit alpha Homo sapiens 27-32 32594352-10 2020 Poor survival outcomes and resistance to Temozolomide therapy were associated with higher NRF1 expression including its targets - LDHA, ZMAT3, NSUN2, ARMC5, NDEL1, CLPTM1L, ALKBH5, YIPF5, PPP2CA, and TFG. Temozolomide 41-53 protein phosphatase 2 catalytic subunit alpha Homo sapiens 188-194 32896083-0 2021 Anti-renal interstitial fibrosis effect of norcantharidin is exerted through inhibition of PP2Ac-mediated C-terminal phosphorylation of Smad3. norcantharidin 43-57 protein phosphatase 2 catalytic subunit alpha Homo sapiens 91-96 33000885-4 2020 In the present study, we aimed to reveal the mechanism that induces the nitration of PP2A catalytic subunit (PP2Ac) during cAMP-induced decidualization of hESCs. Cyclic AMP 123-127 protein phosphatase 2 catalytic subunit alpha Homo sapiens 85-107 33000885-4 2020 In the present study, we aimed to reveal the mechanism that induces the nitration of PP2A catalytic subunit (PP2Ac) during cAMP-induced decidualization of hESCs. Cyclic AMP 123-127 protein phosphatase 2 catalytic subunit alpha Homo sapiens 109-114 33000885-5 2020 First, cAMP-induced PP2Ac nitration was significantly repressed using L-NAME, an inhibitor of nitric oxide synthase (NOS). Cyclic AMP 7-11 protein phosphatase 2 catalytic subunit alpha Homo sapiens 20-25 33000885-5 2020 First, cAMP-induced PP2Ac nitration was significantly repressed using L-NAME, an inhibitor of nitric oxide synthase (NOS). NG-Nitroarginine Methyl Ester 70-76 protein phosphatase 2 catalytic subunit alpha Homo sapiens 20-25 33000885-7 2020 Second, cAMP-induced iNOS expression and PP2Ac nitration were decreased by treatment with TSA, an inhibitor of histone deacetylase 5 (HDAC5). Cyclic AMP 8-12 protein phosphatase 2 catalytic subunit alpha Homo sapiens 41-46 33000885-7 2020 Second, cAMP-induced iNOS expression and PP2Ac nitration were decreased by treatment with TSA, an inhibitor of histone deacetylase 5 (HDAC5). trichostatin A 90-93 protein phosphatase 2 catalytic subunit alpha Homo sapiens 41-46 33162896-4 2020 AngII treatment reduced the levels of phosphorylation of eNOS Ser1177 and nitric oxide (NO) content along with phosphorylation of PP2Ac (PP2A catalytic subunit) Tyr307, meanwhile increased the PP2A activity and ROS production in human umbilical vein endothelial cells (HUVECs). Reactive Oxygen Species 211-214 protein phosphatase 2 catalytic subunit alpha Homo sapiens 130-135 33162896-9 2020 The pretreatments of antioxidant N-acetylcysteine (NAC) and apocynin (APO) abolished the drop of the levels of PP2Ac Tyr307 and eNOS Ser1177 induced by AngII in HUVECs. Acetylcysteine 33-49 protein phosphatase 2 catalytic subunit alpha Homo sapiens 111-116 33162896-9 2020 The pretreatments of antioxidant N-acetylcysteine (NAC) and apocynin (APO) abolished the drop of the levels of PP2Ac Tyr307 and eNOS Ser1177 induced by AngII in HUVECs. Acetylcysteine 51-54 protein phosphatase 2 catalytic subunit alpha Homo sapiens 111-116 33162896-9 2020 The pretreatments of antioxidant N-acetylcysteine (NAC) and apocynin (APO) abolished the drop of the levels of PP2Ac Tyr307 and eNOS Ser1177 induced by AngII in HUVECs. acetovanillone 60-68 protein phosphatase 2 catalytic subunit alpha Homo sapiens 111-116 33162896-9 2020 The pretreatments of antioxidant N-acetylcysteine (NAC) and apocynin (APO) abolished the drop of the levels of PP2Ac Tyr307 and eNOS Ser1177 induced by AngII in HUVECs. acetovanillone 70-73 protein phosphatase 2 catalytic subunit alpha Homo sapiens 111-116 33162896-12 2020 In summary, our findings indicate that AngII downregulates PP2A catalytic subunit Tyr307 phosphorylation to activate PP2A via AT1R-mediated Nox/ROS signaling pathway. Reactive Oxygen Species 144-147 protein phosphatase 2 catalytic subunit alpha Homo sapiens 59-81 30696301-1 2019 Several ellagitannins inhibited the activity of protein phosphatase-1 (PP1) and -2 A (PP2A) catalytic subunits (PP1c and PP2Ac) with preferential suppression of PP1c over PP2Ac. Hydrolyzable Tannins 8-21 protein phosphatase 2 catalytic subunit alpha Homo sapiens 121-126 32481626-7 2020 Bioinformatics analyses highlighted the serine/threonine-protein phosphatases PPP2CA, responsible for GSK-3beta activation, among the miR-675-5p targets, thus indicating the molecular mediator through which miR-675-5p may control beta-catenin nuclear localization. Serine 40-46 protein phosphatase 2 catalytic subunit alpha Homo sapiens 78-84 32130916-2 2020 Phosphorylation of the PP2A catalytic subunit (PP2AC) at tyrosine 307 has been claimed to inactivate PP2A and was examined in more than 180 studies using commercial antibodies, but this modification was never identified using mass spectrometry. Tyrosine 57-65 protein phosphatase 2 catalytic subunit alpha Homo sapiens 23-45 32130916-2 2020 Phosphorylation of the PP2A catalytic subunit (PP2AC) at tyrosine 307 has been claimed to inactivate PP2A and was examined in more than 180 studies using commercial antibodies, but this modification was never identified using mass spectrometry. Tyrosine 57-65 protein phosphatase 2 catalytic subunit alpha Homo sapiens 47-52 32130916-3 2020 Here we show that the most cited pTyr307 monoclonal antibodies, E155 and F-8, are not specific for phosphorylated Tyr307 but instead are hampered by PP2AC methylation at leucine 309 or phosphorylation at threonine 304. ptyr307 33-40 protein phosphatase 2 catalytic subunit alpha Homo sapiens 149-154 32130916-3 2020 Here we show that the most cited pTyr307 monoclonal antibodies, E155 and F-8, are not specific for phosphorylated Tyr307 but instead are hampered by PP2AC methylation at leucine 309 or phosphorylation at threonine 304. Leucine 170-177 protein phosphatase 2 catalytic subunit alpha Homo sapiens 149-154 32130916-3 2020 Here we show that the most cited pTyr307 monoclonal antibodies, E155 and F-8, are not specific for phosphorylated Tyr307 but instead are hampered by PP2AC methylation at leucine 309 or phosphorylation at threonine 304. Threonine 204-213 protein phosphatase 2 catalytic subunit alpha Homo sapiens 149-154 32130916-4 2020 Other pTyr307 antibodies are sensitive to PP2AC methylation as well, and some cross-react with pTyr residues in general, including phosphorylated hemagglutinin tags. ptyr307 6-13 protein phosphatase 2 catalytic subunit alpha Homo sapiens 42-47 32130916-4 2020 Other pTyr307 antibodies are sensitive to PP2AC methylation as well, and some cross-react with pTyr residues in general, including phosphorylated hemagglutinin tags. Phosphotyrosine 6-10 protein phosphatase 2 catalytic subunit alpha Homo sapiens 42-47 32130916-5 2020 We identify pTyr307 using targeted mass spectrometry after transient overexpression of PP2AC and Src kinase. ptyr307 12-19 protein phosphatase 2 catalytic subunit alpha Homo sapiens 87-92 30696301-1 2019 Several ellagitannins inhibited the activity of protein phosphatase-1 (PP1) and -2 A (PP2A) catalytic subunits (PP1c and PP2Ac) with preferential suppression of PP1c over PP2Ac. Hydrolyzable Tannins 8-21 protein phosphatase 2 catalytic subunit alpha Homo sapiens 171-176 31792377-5 2019 Following serum or lysophosphatidic acid stimulation, active RhoA binds and dissociates rhophilin and NF2/Kibra from STRIPAK, thereby inducing the association and dephosphorylation of MST1/2 and MAP4Ks by the STRIPAK phosphatase catalytic subunit PP2AC. lysophosphatidic acid 19-40 protein phosphatase 2 catalytic subunit alpha Homo sapiens 247-252 30852180-0 2019 Cucurbitacin B induces inhibitory effects via the CIP2A/PP2A/C-KIT signaling axis in t(8;21) acute myeloid leukemia. cucurbitacin B 0-14 protein phosphatase 2 catalytic subunit alpha Homo sapiens 56-62 30898057-3 2019 METHODS: In the previous study, we have showed that LCMT1-dependent PP2Ac methylation arrests H2O2-induced cell oxidative stress damage. Hydrogen Peroxide 94-98 protein phosphatase 2 catalytic subunit alpha Homo sapiens 68-73 31085588-5 2019 Overexpression of the PP2A catalytic subunit (PP2Ac) by lentiviral transduction in human Tregs increased the level of IL-2R subunits and promoted tyrosine phosphorylation of Jak3 and STAT5. Tyrosine 146-154 protein phosphatase 2 catalytic subunit alpha Homo sapiens 22-44 31085588-5 2019 Overexpression of the PP2A catalytic subunit (PP2Ac) by lentiviral transduction in human Tregs increased the level of IL-2R subunits and promoted tyrosine phosphorylation of Jak3 and STAT5. Tyrosine 146-154 protein phosphatase 2 catalytic subunit alpha Homo sapiens 46-51 30340029-10 2018 Antioxidant N-acetyl cysteine can reverse the nuclear to cytoplasmic ratio of PME-1 proteins and dem-p-PP2Ac after H2O2 exposure. Acetylcysteine 12-29 protein phosphatase 2 catalytic subunit alpha Homo sapiens 103-108 30521379-7 2019 To devise an endothelial-targeted anti-PP2Ac nitration strategy, a mimic peptide, tyrosine 265 wild type (Y265WT), conjugated with the cell-penetrating peptide HIV-1 TAT protein (TAT) was synthesized. Tyrosine 82-90 protein phosphatase 2 catalytic subunit alpha Homo sapiens 39-44 30679389-0 2019 The Antitumor Drug LB-100 Is a Catalytic Inhibitor of Protein Phosphatase 2A (PPP2CA) and 5 (PPP5C) Coordinating with the Active-Site Catalytic Metals in PPP5C. lb100 19-25 protein phosphatase 2 catalytic subunit alpha Homo sapiens 78-91 30679389-3 2019 Although LB-100 is widely reported as a specific inhibitor of serine/threonine phosphatase 2A (PP2AC/PPP2CA:PPP2CB), we could find no experimental evidence in the published literature demonstrating the specific engagement of LB-100 with PP2A in vitro, in cultured cells, or in animals. lb100 9-15 protein phosphatase 2 catalytic subunit alpha Homo sapiens 95-100 30679389-3 2019 Although LB-100 is widely reported as a specific inhibitor of serine/threonine phosphatase 2A (PP2AC/PPP2CA:PPP2CB), we could find no experimental evidence in the published literature demonstrating the specific engagement of LB-100 with PP2A in vitro, in cultured cells, or in animals. lb100 9-15 protein phosphatase 2 catalytic subunit alpha Homo sapiens 101-107 30679389-3 2019 Although LB-100 is widely reported as a specific inhibitor of serine/threonine phosphatase 2A (PP2AC/PPP2CA:PPP2CB), we could find no experimental evidence in the published literature demonstrating the specific engagement of LB-100 with PP2A in vitro, in cultured cells, or in animals. Serine 62-68 protein phosphatase 2 catalytic subunit alpha Homo sapiens 95-100 30679389-4 2019 Rather, the premise for LB-100 targeting PP2AC is derived from studies that measure phosphate released from a phosphopeptide (K-R-pT-I-R-R) or inferred from the ability of LB-100 to mimic activity previously reported to result from the inhibition of PP2AC by other means. Phosphates 84-93 protein phosphatase 2 catalytic subunit alpha Homo sapiens 41-46 30679389-4 2019 Rather, the premise for LB-100 targeting PP2AC is derived from studies that measure phosphate released from a phosphopeptide (K-R-pT-I-R-R) or inferred from the ability of LB-100 to mimic activity previously reported to result from the inhibition of PP2AC by other means. Phosphopeptides 110-124 protein phosphatase 2 catalytic subunit alpha Homo sapiens 41-46 30679389-7 2019 Inhibition assays using purified enzymes demonstrate that LB-100 is a catalytic inhibitor of both PP2AC and PPP5C. lb100 58-64 protein phosphatase 2 catalytic subunit alpha Homo sapiens 98-103 30679389-8 2019 The structure of PPP5C cocrystallized with LB-100 was solved to a resolution of 1.65A, revealing that the 7-oxabicyclo[2.2.1]heptane-2,3-dicarbonyl moiety coordinates with the metal ions and key residues that are conserved in both PP2AC and PPP5C. 7-oxabicyclo[2.2.1]heptane-2,3-dicarbonyl 106-147 protein phosphatase 2 catalytic subunit alpha Homo sapiens 231-236 30679389-9 2019 Cell-based studies revealed some known actions of LB-100 are mimicked by the genetic disruption of PPP5C These data demonstrate that LB-100 is a catalytic inhibitor of both PP2AC and PPP5C and suggest that the observed antitumor activity might be due to an additive effect achieved by suppressing both PP2A and PPP5C. lb100 50-56 protein phosphatase 2 catalytic subunit alpha Homo sapiens 173-178 31065512-7 2019 RP/C full cells using potassium manganese hexacyanoferrate as cathode show a long cycling life (680 cycles) at a current density of 1000 mA g-1, in addition, a pouch-type battery is built to demonstrate practical applications. potassium manganese hexacyanoferrate 22-58 protein phosphatase 2 catalytic subunit alpha Homo sapiens 0-4 30693913-6 2019 RESULTS Metformin treatment significantly reduced A549 or H1651 cell growth and invasive capacity in vitro as well as Ser184 phosphorylation of Bax, Ser62 phosphorylation of Myc, and Ser473 phosphorylation of Akt, all of which could be partially attenuated by OA treatment, O/E alpha4 or sh-PP2Ac. Metformin 8-17 protein phosphatase 2 catalytic subunit alpha Homo sapiens 291-296 30693913-7 2019 Metformin treatment also significantly reduced tumor formation in vivo as well as protein expression of PCNA, Akt, Myc, and serine phosphorylation of the latter 2, which can be partially blocked by O/E alpha4 or sh-PP2Ac. Metformin 0-9 protein phosphatase 2 catalytic subunit alpha Homo sapiens 215-220 30693913-7 2019 Metformin treatment also significantly reduced tumor formation in vivo as well as protein expression of PCNA, Akt, Myc, and serine phosphorylation of the latter 2, which can be partially blocked by O/E alpha4 or sh-PP2Ac. Serine 124-130 protein phosphatase 2 catalytic subunit alpha Homo sapiens 215-220 30679389-9 2019 Cell-based studies revealed some known actions of LB-100 are mimicked by the genetic disruption of PPP5C These data demonstrate that LB-100 is a catalytic inhibitor of both PP2AC and PPP5C and suggest that the observed antitumor activity might be due to an additive effect achieved by suppressing both PP2A and PPP5C. lb100 133-139 protein phosphatase 2 catalytic subunit alpha Homo sapiens 173-178 30693913-3 2019 Influences of okadaic acid (OA) treatment, O/E alpha4 or sh-PP2Ac on metformin treated cells were investigated by cell viability, proliferation, apoptosis, and Transwell invasion assay in vitro. Metformin 69-78 protein phosphatase 2 catalytic subunit alpha Homo sapiens 60-65 30340029-10 2018 Antioxidant N-acetyl cysteine can reverse the nuclear to cytoplasmic ratio of PME-1 proteins and dem-p-PP2Ac after H2O2 exposure. Hydrogen Peroxide 115-119 protein phosphatase 2 catalytic subunit alpha Homo sapiens 103-108 29317521-6 2018 Plasma membrane depolarization, PP2Ac demethylation, and cell death were prevented by glucose and, unexpectedly, by its nonmetabolizable analog 2-deoxy-d-glucose (2-DG), a glycolytic inhibitor. Glucose 86-93 protein phosphatase 2 catalytic subunit alpha Homo sapiens 32-37 30381122-10 2018 Compared with the DMSO group, PP2Ac demethylation decreased while spindle cells, phagocytosis, and the mRNA levels of COX-2, TNF-alpha, IL-6 and CXCL10 significantly increased in the administration group with 0.5 mumol/L ABL127. Dimethyl Sulfoxide 18-22 protein phosphatase 2 catalytic subunit alpha Homo sapiens 30-35 29228318-2 2018 The present study investigated the role of PP2A catalytic subunit (PP2Ac) demethylation and its link with glycogen synthase kinase-3beta (GSK)-3beta in tau hyperphosphorylation induced by MC-LR. mc-lr 188-193 protein phosphatase 2 catalytic subunit alpha Homo sapiens 43-65 29228318-3 2018 The results showed that MC-LR treatment significantly increased demethylation of PP2Ac, with a concomitant increase in GSK-3beta phosphorylation at Ser9 resulting in elevated tau hyperphosphorylation at PP2A-favorable sites in SH-SY5Y cells and rat hippocampus. mc-lr 24-29 protein phosphatase 2 catalytic subunit alpha Homo sapiens 81-86 29228318-4 2018 Coimmunoprecipitation experiments showed that MC-LR treatment dissociated PP2Ac from Balpha, making it incompetent in binding tau, thus causing tau hyperphosphorylation. mc-lr 46-51 protein phosphatase 2 catalytic subunit alpha Homo sapiens 74-79 29228318-5 2018 Moreover, we found that inhibition of PP2A resulted in an increase in phosphorylation of GSK-3beta at Ser9 and a decrease in GSK-3beta activity, which further promoted demethylation of PP2Ac induced by MC-LR. mc-lr 202-207 protein phosphatase 2 catalytic subunit alpha Homo sapiens 185-190 29228318-6 2018 These findings suggest a scenario in which MC-LR-mediated demethylation of PP2Ac is associated with GSK-3beta phosphorylation at Ser9 and contributes to dissociation of Balpha from PP2Ac, which would result in Balpha degradation and disruption of PP2A/Balpha-tau interactions, thus promoting tau hyperphosphorylation and paired helical filaments-tau accumulation and, consequently, axonal degeneration and cell death. mc-lr 43-48 protein phosphatase 2 catalytic subunit alpha Homo sapiens 75-80 29228318-6 2018 These findings suggest a scenario in which MC-LR-mediated demethylation of PP2Ac is associated with GSK-3beta phosphorylation at Ser9 and contributes to dissociation of Balpha from PP2Ac, which would result in Balpha degradation and disruption of PP2A/Balpha-tau interactions, thus promoting tau hyperphosphorylation and paired helical filaments-tau accumulation and, consequently, axonal degeneration and cell death. mc-lr 43-48 protein phosphatase 2 catalytic subunit alpha Homo sapiens 181-186 29317521-6 2018 Plasma membrane depolarization, PP2Ac demethylation, and cell death were prevented by glucose and, unexpectedly, by its nonmetabolizable analog 2-deoxy-d-glucose (2-DG), a glycolytic inhibitor. Deoxyglucose 144-161 protein phosphatase 2 catalytic subunit alpha Homo sapiens 32-37 28760745-5 2017 Enhanced expression and membrane targeting of PP2A-C was observed in 5% O2, resulting in greater interaction with eNOS in response to histamine. Oxygen 72-74 protein phosphatase 2 catalytic subunit alpha Homo sapiens 46-52 28760745-5 2017 Enhanced expression and membrane targeting of PP2A-C was observed in 5% O2, resulting in greater interaction with eNOS in response to histamine. Histamine 134-143 protein phosphatase 2 catalytic subunit alpha Homo sapiens 46-52 28760745-6 2017 Moreover, increased dephosphorylation of eNOS in 5% O2 was Ca2+-sensitive and reversed by okadaic acid or PP2A-C siRNA. Oxygen 52-54 protein phosphatase 2 catalytic subunit alpha Homo sapiens 106-112 26784437-5 2017 The results showed that PP2A activity decreased and PP2A/C subunit expression and phosphorylation at Tyr307 increased significantly in the group exposed to high MC-LR. cyanoginosin LR 161-166 protein phosphatase 2 catalytic subunit alpha Homo sapiens 52-58 29186909-10 2017 In addition, the pp2a siRNA potently reversed the ketamine-activated catalytic subunit (PP2A-C) of PP2A. Ketamine 50-58 protein phosphatase 2 catalytic subunit alpha Homo sapiens 88-94 28671332-8 2017 Betaine decreased the levels of phosphorylated tau, elevated PP2Ac activity and inhibited Abeta accumulation (P<.05). Betaine 0-7 protein phosphatase 2 catalytic subunit alpha Homo sapiens 61-66 28438603-0 2017 PP2A catalytic subunit silence by microRNA-429 activates AMPK and protects osteoblastic cells from dexamethasone. Dexamethasone 99-112 protein phosphatase 2 catalytic subunit alpha Homo sapiens 0-4 28382174-5 2017 ROS increased the stability and activity of PP2Ac, resulting in decreased AKT activity. Reactive Oxygen Species 0-3 protein phosphatase 2 catalytic subunit alpha Homo sapiens 44-49 27612201-7 2016 Moreover, inhibition of PP2A by okadaic acid (OA), a specific inhibitor of PP2A phosphatase activity or by depletion of its catalytic subunit (PP2Ac), led to a significant inactivation of Raf-MEK-ERK signaling and reduced glutaminolysis in endothelial cells. Okadaic Acid 46-48 protein phosphatase 2 catalytic subunit alpha Homo sapiens 143-148 26506538-4 2016 The results showed that there were no obvious difference in apoptosis rate and cell-cycle distribution but the cell proliferation was changed since 36 h exposure while the uptake of MC-LR and its binding to PP2A/C kept unchanged since 1h exposure. cyanoginosin LR 182-187 protein phosphatase 2 catalytic subunit alpha Homo sapiens 207-213 27012437-6 2016 Rotenone increased the phosphorylation of alpha-syn at Ser129, consistent with the inhibition of PP2A activity by increased phosphorylation of tyrosine 307 at the catalytic subunit of PP2A (pTyr307 PP2Ac). Rotenone 0-8 protein phosphatase 2 catalytic subunit alpha Homo sapiens 198-203 27012437-8 2016 Rotenone inhibited PP2A activity via phosphorylation of PP2Ac at Tyr307. Rotenone 0-8 protein phosphatase 2 catalytic subunit alpha Homo sapiens 19-23 27012437-8 2016 Rotenone inhibited PP2A activity via phosphorylation of PP2Ac at Tyr307. Rotenone 0-8 protein phosphatase 2 catalytic subunit alpha Homo sapiens 56-61 27012437-9 2016 The reduction in PP2A activity and rotenone cytotoxicity were reversed by treatment with the PP2A agonist, C2 ceramide, and the Src kinase inhibitor, SKI606. Rotenone 35-43 protein phosphatase 2 catalytic subunit alpha Homo sapiens 93-97 27012437-9 2016 The reduction in PP2A activity and rotenone cytotoxicity were reversed by treatment with the PP2A agonist, C2 ceramide, and the Src kinase inhibitor, SKI606. N-acetylsphingosine 107-118 protein phosphatase 2 catalytic subunit alpha Homo sapiens 17-21 27012437-10 2016 Immunoprecipitation experiments showed that rotenone induced an increase in calmodulin-Src complex in SK-N-SH cells, thus activating Src kinase, which in turn phosphorylated PP2A at Tyr307 and inhibited its activity. Rotenone 44-52 protein phosphatase 2 catalytic subunit alpha Homo sapiens 174-178 27012437-11 2016 C2 ceramide and SKI606 significantly reversed the rotenone-induced phosphorylation and aggregation of alpha-syn by increasing PP2A activity. Ceramides 3-11 protein phosphatase 2 catalytic subunit alpha Homo sapiens 126-130 27012437-11 2016 C2 ceramide and SKI606 significantly reversed the rotenone-induced phosphorylation and aggregation of alpha-syn by increasing PP2A activity. Rotenone 50-58 protein phosphatase 2 catalytic subunit alpha Homo sapiens 126-130 27012437-12 2016 These results demonstrate that rotenone-reduced PP2A activity via Src kinase is involved in the phosphorylation of alpha-syn. Rotenone 31-39 protein phosphatase 2 catalytic subunit alpha Homo sapiens 48-52 27013170-4 2016 METHODS: Corticosteroid sensitivity, GR nuclear translocation, phosphorylation levels of GR-Ser(226), JNK1 and PP2A catalytic subunit (PP2AC)-Tyr(307) and protein expression levels and activities of PTP-RR and PP2AC were evaluated in U937 cells and/or peripheral blood mononuclear cells (PBMCs). Tyrosine 142-145 protein phosphatase 2 catalytic subunit alpha Homo sapiens 135-140 27334924-5 2016 Truncation and alanine mutagenesis studies revealed that PP2Ac binds to the P3 block ((396)PAIPPKKPRP(405)) of the proline-rich region in CIN85. Alanine 15-22 protein phosphatase 2 catalytic subunit alpha Homo sapiens 57-62 27334924-5 2016 Truncation and alanine mutagenesis studies revealed that PP2Ac binds to the P3 block ((396)PAIPPKKPRP(405)) of the proline-rich region in CIN85. Proline 115-122 protein phosphatase 2 catalytic subunit alpha Homo sapiens 57-62 26506538-5 2016 PP2A activity had not manifested continued decline compare to 24h exposure and PP2A regulator alpha4 was found to release its associated PP2A/C since 1h exposure. Hydrogen 150-152 protein phosphatase 2 catalytic subunit alpha Homo sapiens 137-143 24997451-1 2014 BACKGROUND: The tumor suppressor protein phosphatase 2A (PP2A) is frequently inactivated in human cancer and phosphorylation of its catalytic subunit (p-PP2A-C) at tyrosine-307 (Y307) has been described to inhibit this phosphatase. Tyrosine 164-172 protein phosphatase 2 catalytic subunit alpha Homo sapiens 153-159 25047463-10 2014 Substantiating our findings, we demonstrated that ATO activates PP2A/C activity by downregulating miR-520h, which results in IKK inactivation, IkappaB-dephosphorylation, NF-kappaB inactivation, and, subsequently, a reduction in CXCR4 expression. Arsenic Trioxide 50-53 protein phosphatase 2 catalytic subunit alpha Homo sapiens 64-70 24997451-11 2014 Antitumoral effects of forskolin-mediated PP2A activation suggest that the analysis of p-PP2A-C Y307 status could be used to identify a subgroup of patients who would benefit from treatments based on PP2A activators. Colforsin 23-32 protein phosphatase 2 catalytic subunit alpha Homo sapiens 89-95 24993084-4 2014 Increased biotin-MC-LR coated sensorchip surface in the Surface Prep unit of Biacore 3000 captured PP1c, PP2Ac and their regulatory proteins including MYPT1, MYPT family TIMAP, inhibitor-2 as well as PP2A-A and -Balpha-subunits from normal and UVA-irradiated HaCaT cell lysates as revealed by dot blot analysis of the recovered proteins. biotin-mc-lr 10-22 protein phosphatase 2 catalytic subunit alpha Homo sapiens 105-110 24993084-6 2014 Partial colocalization of the biotin-MC-LR signals with those obtained using anti-PP1c and anti-PP2Ac antibodies was apparent as judged by confocal microscopy. biotin-mc-lr 30-42 protein phosphatase 2 catalytic subunit alpha Homo sapiens 96-101 24508028-6 2014 For MKN28 cells diDCP-LA-PI-induced reduction of MEK phosphorylation and cell viability was prevented by knocking-down PP2Ac. didcp-la 16-24 protein phosphatase 2 catalytic subunit alpha Homo sapiens 119-124 24145130-6 2014 However, treatment with proteasomal inhibitor MG132 showed polyubiquitinated-PP2Ac molecules (~65-250kDa) abundantly in siNT controls but low in siEDD-transfectants, implicating PP2Ac as an EDD substrate. benzyloxycarbonylleucyl-leucyl-leucine aldehyde 46-51 protein phosphatase 2 catalytic subunit alpha Homo sapiens 77-82 24632852-7 2014 Consistent with this, PA reduced PP2Ac demethylation and tyrosine307phosphorylation - events associated with PP2A activation. Palmitates 22-24 protein phosphatase 2 catalytic subunit alpha Homo sapiens 33-38 24632852-8 2014 In contrast, OA and LOA strongly opposed these PA-induced changes in PP2Ac thus exerting a repressive effect on PP2A. Palmitates 47-49 protein phosphatase 2 catalytic subunit alpha Homo sapiens 69-74 24145130-6 2014 However, treatment with proteasomal inhibitor MG132 showed polyubiquitinated-PP2Ac molecules (~65-250kDa) abundantly in siNT controls but low in siEDD-transfectants, implicating PP2Ac as an EDD substrate. benzyloxycarbonylleucyl-leucyl-leucine aldehyde 46-51 protein phosphatase 2 catalytic subunit alpha Homo sapiens 178-183 23428800-2 2013 Here, we studied whether phosphotyrosyl phosphatase activator (PTPA) could activate PP2A by reducing its inhibitory phosphorylation at tyrosine 307 (P-PP2AC). Tyrosine 135-143 protein phosphatase 2 catalytic subunit alpha Homo sapiens 151-156 24100030-5 2013 We also show that upon TRAIL treatment, Src, caspase-8, and PP2A/C (a catalytic subunit of the PP2A phosphatase) are redistributed into lipid rafts, a microdomain of the plasma membrane enriched with cholesterol, where PP2A dephosphorylates Src at tyrosine 418 and in turn inhibits caspase-8 phosphorylation. Cholesterol 200-211 protein phosphatase 2 catalytic subunit alpha Homo sapiens 60-66 24100030-5 2013 We also show that upon TRAIL treatment, Src, caspase-8, and PP2A/C (a catalytic subunit of the PP2A phosphatase) are redistributed into lipid rafts, a microdomain of the plasma membrane enriched with cholesterol, where PP2A dephosphorylates Src at tyrosine 418 and in turn inhibits caspase-8 phosphorylation. Tyrosine 248-256 protein phosphatase 2 catalytic subunit alpha Homo sapiens 60-66 23630944-6 2013 In contrast, mutant Atxn1 induces a decrease in Y307-phosphorylation in Pp2a-c, known to enhance its activity, while reducing Pp2a-b expression and inhibiting Anp32a levels. y307 48-52 protein phosphatase 2 catalytic subunit alpha Homo sapiens 72-78 23583701-6 2013 Results revealed that an oxidative stress-induced PTK/PTP imbalance led to phosphorylation of PP2Ac, following exposures to t-BHP, AAPH, and Na3VO4 in HEK293T cells. tert-Butylhydroperoxide 124-129 protein phosphatase 2 catalytic subunit alpha Homo sapiens 94-99 23775084-5 2013 We show that both chemical suppression and siRNA silencing of PP2Ac in T-cells resulted in sustained phosphorylation of MEK and ERK following stimulation with phorbol 12-myristate 13-acetate and ionomycin. Tetradecanoylphorbol Acetate 159-190 protein phosphatase 2 catalytic subunit alpha Homo sapiens 62-67 23775084-5 2013 We show that both chemical suppression and siRNA silencing of PP2Ac in T-cells resulted in sustained phosphorylation of MEK and ERK following stimulation with phorbol 12-myristate 13-acetate and ionomycin. Ionomycin 195-204 protein phosphatase 2 catalytic subunit alpha Homo sapiens 62-67 24395787-3 2014 We found a decrease in protein phosphatase 2A (PP2A) activity associated with an increase in inhibitory phosphorylation of its catalytic subunit PP2Ac at Tyr(307) and abnormal hyperphosphorylation of tau in brains of patients who had Guam PD. Tyrosine 154-157 protein phosphatase 2 catalytic subunit alpha Homo sapiens 145-150 24395787-6 2014 Moreover, we found an increase in the phosphorylation of PP2Ac at Tyr(307) in BMAA-treated rat brains. Tyrosine 66-69 protein phosphatase 2 catalytic subunit alpha Homo sapiens 57-62 24395787-6 2014 Moreover, we found an increase in the phosphorylation of PP2Ac at Tyr(307) in BMAA-treated rat brains. beta-N-methylamino-L-alanine 78-82 protein phosphatase 2 catalytic subunit alpha Homo sapiens 57-62 24395787-8 2014 Coimmunoprecipitation experiments showed that BMAA treatment dissociated PP2Ac from mGluR5, making it available for phosphorylation at Tyr(307). beta-N-methylamino-L-alanine 46-50 protein phosphatase 2 catalytic subunit alpha Homo sapiens 73-78 24395787-8 2014 Coimmunoprecipitation experiments showed that BMAA treatment dissociated PP2Ac from mGluR5, making it available for phosphorylation at Tyr(307). Tyrosine 135-138 protein phosphatase 2 catalytic subunit alpha Homo sapiens 73-78 24395787-9 2014 These findings suggest a scenario in which BMAA can lead to tau pathology by inhibiting PP2A through the activation of mGluR5, the consequent release of PP2Ac from the mGluR5-PP2A complex, and its phosphorylation at Tyr(307) by Src. beta-N-methylamino-L-alanine 43-47 protein phosphatase 2 catalytic subunit alpha Homo sapiens 153-158 24395787-9 2014 These findings suggest a scenario in which BMAA can lead to tau pathology by inhibiting PP2A through the activation of mGluR5, the consequent release of PP2Ac from the mGluR5-PP2A complex, and its phosphorylation at Tyr(307) by Src. Tyrosine 216-219 protein phosphatase 2 catalytic subunit alpha Homo sapiens 153-158 25343293-8 2014 In addition, co-treatment with paclitaxel and clusterin siRNA significantly enhanced PP2AC levels. Paclitaxel 31-41 protein phosphatase 2 catalytic subunit alpha Homo sapiens 85-90 23583701-6 2013 Results revealed that an oxidative stress-induced PTK/PTP imbalance led to phosphorylation of PP2Ac, following exposures to t-BHP, AAPH, and Na3VO4 in HEK293T cells. 2,2'-azobis(2-amidinopropane) 131-135 protein phosphatase 2 catalytic subunit alpha Homo sapiens 94-99 23583701-6 2013 Results revealed that an oxidative stress-induced PTK/PTP imbalance led to phosphorylation of PP2Ac, following exposures to t-BHP, AAPH, and Na3VO4 in HEK293T cells. Sodium orthovanadate 141-147 protein phosphatase 2 catalytic subunit alpha Homo sapiens 94-99 22260360-3 2013 EGCG and PGG inhibit PP2Ac less potently, with IC(50) values of 15 and 6.6 mum, respectively. epigallocatechin gallate 0-4 protein phosphatase 2 catalytic subunit alpha Homo sapiens 21-26 22961627-6 2013 Activation of PP2A by overexpression of PP2AC or PR65 led to cytoplasmic accumulation of hTERT, which was reversed by treatment with PP2A inhibitor okadaic acid. Okadaic Acid 148-160 protein phosphatase 2 catalytic subunit alpha Homo sapiens 40-45 22410781-5 2013 We also identified a signal cascade, namely, resveratrol- miRNA-520h- PP2A/C- Akt NF-kappaB FOXC2, in which resveratrol inhibited the expression of FOXC2 through regulation of miRNA-520h-mediated signal cascade. mirna-520h 180-190 protein phosphatase 2 catalytic subunit alpha Homo sapiens 70-76 22410781-6 2013 This study identified a new miRNA-520h-related signal cascade involved in resveratrol-mediated tumor suppression activity and provide the clinical significances of miR-520h, PP2A/C and FOXC2 in lung cancer patients. Resveratrol 74-85 protein phosphatase 2 catalytic subunit alpha Homo sapiens 174-180 23518861-5 2013 RESULTS: The expression of PP2A catalytic subunit, (PP2Ac) was reduced in A2780/CDDP as well as in cisplatin-resistant patients" tissues compared with A2780 and cisplatin-sensitive patients. Cisplatin 99-108 protein phosphatase 2 catalytic subunit alpha Homo sapiens 52-57 23518861-5 2013 RESULTS: The expression of PP2A catalytic subunit, (PP2Ac) was reduced in A2780/CDDP as well as in cisplatin-resistant patients" tissues compared with A2780 and cisplatin-sensitive patients. Cisplatin 161-170 protein phosphatase 2 catalytic subunit alpha Homo sapiens 52-57 23518861-10 2013 Our experiments demonstrated that apoptosis suppressed by the knocking down of PP2Ac can be reversed by the administration of 3-methyladenine. 3-methyladenine 126-141 protein phosphatase 2 catalytic subunit alpha Homo sapiens 79-84 23518861-13 2013 CONCLUSIONS: Taken together, our results showed that protective autophagy regulated by PP2Ac is at least part of the mechanism to how certain ovarian cancers are resistant to cisplatin. Cisplatin 175-184 protein phosphatase 2 catalytic subunit alpha Homo sapiens 87-92 22410781-5 2013 We also identified a signal cascade, namely, resveratrol- miRNA-520h- PP2A/C- Akt NF-kappaB FOXC2, in which resveratrol inhibited the expression of FOXC2 through regulation of miRNA-520h-mediated signal cascade. Resveratrol 45-56 protein phosphatase 2 catalytic subunit alpha Homo sapiens 70-76 22410781-5 2013 We also identified a signal cascade, namely, resveratrol- miRNA-520h- PP2A/C- Akt NF-kappaB FOXC2, in which resveratrol inhibited the expression of FOXC2 through regulation of miRNA-520h-mediated signal cascade. mirna-520h 58-68 protein phosphatase 2 catalytic subunit alpha Homo sapiens 70-76 22410781-5 2013 We also identified a signal cascade, namely, resveratrol- miRNA-520h- PP2A/C- Akt NF-kappaB FOXC2, in which resveratrol inhibited the expression of FOXC2 through regulation of miRNA-520h-mediated signal cascade. Resveratrol 112-123 protein phosphatase 2 catalytic subunit alpha Homo sapiens 70-76 24454482-11 2013 However, CIG significantly elevated the activity of PP2A by reducing the demethylation of PP2A catalytic subunit (PP2Ac) at Leu309 and the ratio of PME-1/LCMT in the WT/GFX-treated cells. 2-AMINO-6-CHLOROPYRAZINE 9-12 protein phosphatase 2 catalytic subunit alpha Homo sapiens 90-112 24454482-11 2013 However, CIG significantly elevated the activity of PP2A by reducing the demethylation of PP2A catalytic subunit (PP2Ac) at Leu309 and the ratio of PME-1/LCMT in the WT/GFX-treated cells. 2-AMINO-6-CHLOROPYRAZINE 9-12 protein phosphatase 2 catalytic subunit alpha Homo sapiens 114-119 22260360-3 2013 EGCG and PGG inhibit PP2Ac less potently, with IC(50) values of 15 and 6.6 mum, respectively. beta-penta-O-galloyl-glucose 9-12 protein phosphatase 2 catalytic subunit alpha Homo sapiens 21-26 22260360-8 2013 Comparative docking shows that EGCG binds to PP2Ac in a similar manner, but in a distinct pose. epigallocatechin gallate 31-35 protein phosphatase 2 catalytic subunit alpha Homo sapiens 45-50 22213051-7 2012 Interestingly, treatment of silibinin suppressed the activation of the AKT Ser473/mTOR pathway in spheroid culture through suppressing the activity of protein phosphatase 2Ac subunit (PP2Ac). Silybin 28-37 protein phosphatase 2 catalytic subunit alpha Homo sapiens 184-189 22732552-3 2012 Here we studied the effects of GSK-3beta on the inhibitory demethylation of PP2A at leucine-309 (dmL309-PP2A(C)). dml309 97-103 protein phosphatase 2 catalytic subunit alpha Homo sapiens 104-111 22732552-4 2012 We found that GSK-3beta regulates dmL309-PP2A(C) level by regulating PME-1 and PPMT1. dml309 34-40 protein phosphatase 2 catalytic subunit alpha Homo sapiens 41-48 22684030-10 2012 On the other hand, anisomycin (4 mumol/L) reduced the level of PP2A/C subunit (catalytic subunit) in a time-dependent manner in the two cell lines. Anisomycin 19-29 protein phosphatase 2 catalytic subunit alpha Homo sapiens 63-69 22684030-13 2012 The regulation of PP2A/C exression by anisomycin provides a clue to further study on its role in glioma therapy. Anisomycin 38-48 protein phosphatase 2 catalytic subunit alpha Homo sapiens 18-24 22213051-9 2012 Silibinin, which inhibits colon CSLCs self-renewal and sphere formation by suppressing the PP2Ac/AKT Ser473/mTOR pathway, may be a compound for developing new strategies in modulating CSLCs in cancer therapy. Silybin 0-9 protein phosphatase 2 catalytic subunit alpha Homo sapiens 91-96 21708940-5 2011 siRNA against PP2A catalytic subunit (PP2Ac) or treatment with pharmacological inhibitor, okadaic acid, enhanced IRAK-1 Lys(48)-linked ubiquitination and degradation. Lysine 120-123 protein phosphatase 2 catalytic subunit alpha Homo sapiens 14-36 22109829-6 2012 Furthermore, FTY720-induced toxicity in AML leukemic cells was mediated by restoration of PP2A activity, via down-regulation of PP2A inhibitor SET, dephosporylation of PP2A-C(TYR307), and up-regulation of relevant PP2A subunit A and B55alpha. Fingolimod Hydrochloride 13-19 protein phosphatase 2 catalytic subunit alpha Homo sapiens 168-174 21708940-5 2011 siRNA against PP2A catalytic subunit (PP2Ac) or treatment with pharmacological inhibitor, okadaic acid, enhanced IRAK-1 Lys(48)-linked ubiquitination and degradation. Lysine 120-123 protein phosphatase 2 catalytic subunit alpha Homo sapiens 38-43 21806989-3 2011 The C-terminal acidic region in I(2CTF) and Val 92 in I(2NTF) are essential for their association with PP2Ac and inhibition of the phosphatase activity. Valine 44-47 protein phosphatase 2 catalytic subunit alpha Homo sapiens 103-108 22131637-2 2011 The method was carried out on a Chromolith RP-C(18) column using a mixture of potassium phosphate buffer and methanol (40:60 v/v) and detection was done at 251 nm. chromolith 32-42 protein phosphatase 2 catalytic subunit alpha Homo sapiens 43-47 21098287-0 2010 Biguanide metformin acts on tau phosphorylation via mTOR/protein phosphatase 2A (PP2A) signaling. Metformin 10-19 protein phosphatase 2 catalytic subunit alpha Homo sapiens 81-85 21098287-6 2010 Affinity chromatography and immunoprecipitation experiments together with PP2A activity assays indicate that metformin interferes with the association of the catalytic subunit of PP2A (PP2Ac) to the so-called MID1-alpha4 protein complex, which regulates the degradation of PP2Ac and thereby influences PP2A activity. Metformin 109-118 protein phosphatase 2 catalytic subunit alpha Homo sapiens 185-190 21098287-0 2010 Biguanide metformin acts on tau phosphorylation via mTOR/protein phosphatase 2A (PP2A) signaling. Biguanides 0-9 protein phosphatase 2 catalytic subunit alpha Homo sapiens 81-85 21098287-6 2010 Affinity chromatography and immunoprecipitation experiments together with PP2A activity assays indicate that metformin interferes with the association of the catalytic subunit of PP2A (PP2Ac) to the so-called MID1-alpha4 protein complex, which regulates the degradation of PP2Ac and thereby influences PP2A activity. Metformin 109-118 protein phosphatase 2 catalytic subunit alpha Homo sapiens 273-278 20599567-6 2010 We found that intracellular inhibition of PP1 and PP2A-like activities by okadaic acid markedly raised the accumulation of Chk2-pT68 without DNA damage induction, and this phenomenon was also seen when PP1-C, PP2A-C, and Wip1/PPM1D were simultaneously knockdown by siRNA. Okadaic Acid 74-86 protein phosphatase 2 catalytic subunit alpha Homo sapiens 209-215 20639120-6 2010 H(2)O(2) also stimulates association of endogenous protein phosphatase 2A catalytic subunit (PP2Ac) with p70-S6K1. Hydrogen Peroxide 0-8 protein phosphatase 2 catalytic subunit alpha Homo sapiens 93-98 19857968-6 2009 Moreover, we directly demonstrated that fostriecin covalently binds to the Cys269 residue of PP2Ac by matrix assisted laser desorption/ionization time-of-flight mass spectrometry analysis. fostriecin 40-50 protein phosphatase 2 catalytic subunit alpha Homo sapiens 93-98 20448038-4 2010 Overexpression of PP2A catalytic subunit (PP2Ac) beta-isoform results in dephosphorylation of MEKK3 at Thr-516 and Ser-520 and termination of MEKK3-mediated NF-kappaB activation. Threonine 103-106 protein phosphatase 2 catalytic subunit alpha Homo sapiens 18-40 20448038-4 2010 Overexpression of PP2A catalytic subunit (PP2Ac) beta-isoform results in dephosphorylation of MEKK3 at Thr-516 and Ser-520 and termination of MEKK3-mediated NF-kappaB activation. Threonine 103-106 protein phosphatase 2 catalytic subunit alpha Homo sapiens 42-47 20212043-11 2010 Phosphatase-2A (PP2A) dephosphorylates Raf-1 Ser-259 in response to mitogens. Serine 45-48 protein phosphatase 2 catalytic subunit alpha Homo sapiens 16-20 21302808-3 2010 Besides, we confirmed PKCalpha played an antiapoptotic role through its effects on ERK1/2 and Akt, while its downregulation was attributed to caspase-3 and PP2Ac under the regulation of ROS. Reactive Oxygen Species 186-189 protein phosphatase 2 catalytic subunit alpha Homo sapiens 156-161 20432471-4 2010 Moreover, caffeine treatment led to MAPK phosphatase-1 (MKP-1) down-regulation and protein phosphatase 2A catalytic subunit (PP2Ac) up-regulation, which were involved in cross-talk between p38 MAPK and ERK. Caffeine 10-18 protein phosphatase 2 catalytic subunit alpha Homo sapiens 91-123 20432471-4 2010 Moreover, caffeine treatment led to MAPK phosphatase-1 (MKP-1) down-regulation and protein phosphatase 2A catalytic subunit (PP2Ac) up-regulation, which were involved in cross-talk between p38 MAPK and ERK. Caffeine 10-18 protein phosphatase 2 catalytic subunit alpha Homo sapiens 125-130 20448038-4 2010 Overexpression of PP2A catalytic subunit (PP2Ac) beta-isoform results in dephosphorylation of MEKK3 at Thr-516 and Ser-520 and termination of MEKK3-mediated NF-kappaB activation. Serine 115-118 protein phosphatase 2 catalytic subunit alpha Homo sapiens 18-40 20448038-4 2010 Overexpression of PP2A catalytic subunit (PP2Ac) beta-isoform results in dephosphorylation of MEKK3 at Thr-516 and Ser-520 and termination of MEKK3-mediated NF-kappaB activation. Serine 115-118 protein phosphatase 2 catalytic subunit alpha Homo sapiens 42-47 20448038-5 2010 PP2Ac associates with the phosphorylated form of MEKK3 and the interaction between PP2Ac and MEKK3 is induced by LPA in a transient fashion in the cells. lysophosphatidic acid 113-116 protein phosphatase 2 catalytic subunit alpha Homo sapiens 0-5 20448038-5 2010 PP2Ac associates with the phosphorylated form of MEKK3 and the interaction between PP2Ac and MEKK3 is induced by LPA in a transient fashion in the cells. lysophosphatidic acid 113-116 protein phosphatase 2 catalytic subunit alpha Homo sapiens 83-88 20448038-6 2010 Furthermore, knockdown of PP2Ac expression enhances LPA-induced MEKK3-mediated IkappaB kinase beta (IKKbeta) phosphorylation and NF-kappaB activation. lysophosphatidic acid 52-55 protein phosphatase 2 catalytic subunit alpha Homo sapiens 26-31 19857968-2 2009 This compound has been predicted to covalently bind to the Cys269 residue of the PP2A catalytic subunit (PP2Ac) at the alpha,beta-unsaturated lactone via a conjugate addition reaction. alpha,beta-unsaturated lactone 119-149 protein phosphatase 2 catalytic subunit alpha Homo sapiens 81-103 19857968-2 2009 This compound has been predicted to covalently bind to the Cys269 residue of the PP2A catalytic subunit (PP2Ac) at the alpha,beta-unsaturated lactone via a conjugate addition reaction. alpha,beta-unsaturated lactone 119-149 protein phosphatase 2 catalytic subunit alpha Homo sapiens 105-110 19501114-6 2009 To obtain further insight into the MC-PP2A interaction, we substituted cysteine at position 269 in PP2Ac with glycine. microcystin 35-37 protein phosphatase 2 catalytic subunit alpha Homo sapiens 99-104 19857968-5 2009 We showed that fostriecin directly binds to PP2Ac in HeLa S3 cells by pull-down assays using bio-Fos. fostriecin 15-25 protein phosphatase 2 catalytic subunit alpha Homo sapiens 44-49 19501114-6 2009 To obtain further insight into the MC-PP2A interaction, we substituted cysteine at position 269 in PP2Ac with glycine. Glycine 110-117 protein phosphatase 2 catalytic subunit alpha Homo sapiens 99-104 19501114-7 2009 The mutant PP2Ac (C269G) was comparable to the wild-type PP2Ac in the hydrolysis of p-NPP, but was more resistant to MCs as indicated by the greater IC(50) values. 4-aminophenylphosphate 84-89 protein phosphatase 2 catalytic subunit alpha Homo sapiens 11-16 19501114-7 2009 The mutant PP2Ac (C269G) was comparable to the wild-type PP2Ac in the hydrolysis of p-NPP, but was more resistant to MCs as indicated by the greater IC(50) values. Microcystins 117-120 protein phosphatase 2 catalytic subunit alpha Homo sapiens 11-16 19366811-4 2009 Among the various regulatory B56 subunits, B56gamma was directly phosphorylated by AMPK at Ser(298) and Ser(336), leading to an increase of PP2A activity through dephosphorylation of PP2Ac at Tyr(307). Serine 104-107 protein phosphatase 2 catalytic subunit alpha Homo sapiens 183-188 19366811-4 2009 Among the various regulatory B56 subunits, B56gamma was directly phosphorylated by AMPK at Ser(298) and Ser(336), leading to an increase of PP2A activity through dephosphorylation of PP2Ac at Tyr(307). Serine 91-94 protein phosphatase 2 catalytic subunit alpha Homo sapiens 183-188 18852266-4 2008 Here, we provide data to support a role for protein phosphatase 2A (PP2A) in the deactivation of SK1 through dephosphorylation of phospho-Ser(225). Serine 138-141 protein phosphatase 2 catalytic subunit alpha Homo sapiens 68-72 19155497-5 2009 After preparing serially truncated PP2Ac alpha promoter luciferase constructs, we found that the region stretching around 240 bases upstream from the translation initiation site was of functional significance and included a cAMP response element motif flanked by three GC boxes. Cyclic AMP 224-228 protein phosphatase 2 catalytic subunit alpha Homo sapiens 35-40 19155497-7 2009 Furthermore, we demonstrated that methylation of deoxycytosine in the CpG islands limited binding of phosphorylated CREB and the activity of the PP2Ac alpha promoter. 2,3-dihydropyrimidin-4-amine 49-62 protein phosphatase 2 catalytic subunit alpha Homo sapiens 145-150 19155497-9 2009 Primary T cells treated with 5-azacitidine, a DNA methyltransferase inhibitor, showed increased expression of PP2Ac alpha mRNA. Azacitidine 29-42 protein phosphatase 2 catalytic subunit alpha Homo sapiens 110-115 19366811-4 2009 Among the various regulatory B56 subunits, B56gamma was directly phosphorylated by AMPK at Ser(298) and Ser(336), leading to an increase of PP2A activity through dephosphorylation of PP2Ac at Tyr(307). Tyrosine 192-195 protein phosphatase 2 catalytic subunit alpha Homo sapiens 183-188 18845789-3 2009 Here we find that specific disruption of protein phosphatase 2A (PP2A) activity by either expression of small t antigen or depletion of PP2A/C by RNA interference enhances Bcl2 phosphorylation and suppresses cisplatin-stimulated p53/Bcl2 binding in association with prolonged cell survival. Cisplatin 208-217 protein phosphatase 2 catalytic subunit alpha Homo sapiens 136-142 18852266-6 2008 Coexpression of PP2Ac with SK1 resulted in reduced Ser(225) phosphorylation of SK1 in human embryonic kidney (HEK293) cells. Serine 51-54 protein phosphatase 2 catalytic subunit alpha Homo sapiens 16-21 18852266-7 2008 In vitro phosphatase assays showed that PP2Ac dephosphorylated both recombinant SK1 and a phosphopeptide based on the phospho-Ser(225) region of SK1. Serine 126-129 protein phosphatase 2 catalytic subunit alpha Homo sapiens 40-45 17537547-3 2008 Homocysteine inhibited the activity of protein phosphatase 2A (PP2A) with a simultaneously increased Leu(309)-demethylation and Tyr(307)-phosphorylation of PP2A catalytic subunit (PP2A(C)). Homocysteine 0-12 protein phosphatase 2 catalytic subunit alpha Homo sapiens 180-187 17537547-4 2008 PP2A(C) Leu(309)-demethylation was positively correlated with its Tyr(307)-phosphorylation; and the abnormally modified PP2A(C) was incompetent in binding to its regulatory subunit (PP2A(B)). Leucine 8-11 protein phosphatase 2 catalytic subunit alpha Homo sapiens 0-7 17537547-4 2008 PP2A(C) Leu(309)-demethylation was positively correlated with its Tyr(307)-phosphorylation; and the abnormally modified PP2A(C) was incompetent in binding to its regulatory subunit (PP2A(B)). Tyrosine 66-69 protein phosphatase 2 catalytic subunit alpha Homo sapiens 0-7 17537547-5 2008 Homocysteine also activated methylesterase which stimulates demethylation of PP2A(C). Homocysteine 0-12 protein phosphatase 2 catalytic subunit alpha Homo sapiens 77-84 17537547-7 2008 A simultaneous supplement of folate and vitamin B12 restored partially the plasma homocysteine level and thus significantly antagonized the homocysteine-induced tau hyperphosphorylation and as well as PP2A inactivation and the activity-related modifications of PP2A(C). Folic Acid 29-35 protein phosphatase 2 catalytic subunit alpha Homo sapiens 261-268 17537547-7 2008 A simultaneous supplement of folate and vitamin B12 restored partially the plasma homocysteine level and thus significantly antagonized the homocysteine-induced tau hyperphosphorylation and as well as PP2A inactivation and the activity-related modifications of PP2A(C). Vitamin B 12 40-51 protein phosphatase 2 catalytic subunit alpha Homo sapiens 261-268 17537547-7 2008 A simultaneous supplement of folate and vitamin B12 restored partially the plasma homocysteine level and thus significantly antagonized the homocysteine-induced tau hyperphosphorylation and as well as PP2A inactivation and the activity-related modifications of PP2A(C). Homocysteine 140-152 protein phosphatase 2 catalytic subunit alpha Homo sapiens 261-268 18586681-11 2008 Inhibition of PAK by overexpression of PP2Calpha or the kinase inhibitory domain prevented sorbitol-induced focal adhesion dissolution. Sorbitol 91-99 protein phosphatase 2 catalytic subunit alpha Homo sapiens 39-48 19031739-10 2008 3) Western blot analysis showed that the level of PP2A catalytic subunit (PP2A/C) was decreased during the course of ATRA-induced NB4 cell differentiation, whereas expressions of every subunits of PP2A in MR2 cells were somewhat unaltered. Tretinoin 117-121 protein phosphatase 2 catalytic subunit alpha Homo sapiens 50-72 19031739-10 2008 3) Western blot analysis showed that the level of PP2A catalytic subunit (PP2A/C) was decreased during the course of ATRA-induced NB4 cell differentiation, whereas expressions of every subunits of PP2A in MR2 cells were somewhat unaltered. Tretinoin 117-121 protein phosphatase 2 catalytic subunit alpha Homo sapiens 74-80 16635493-4 2006 In contrast to ethylene glycol, the nonpolar solvent cyclohexane was observed to be well mixed with the RP-C18 stationary phase. Cyclohexane 53-64 protein phosphatase 2 catalytic subunit alpha Homo sapiens 104-108 18299321-9 2008 Attenuation of PP2A activity by OA treatment or targeted knockdown of PP2A(C) with small interfering RNA enhances TGF-beta1-induced phosphorylation of TAK1 at Thr-187 and MKK3 (MAPK kinase 3). Threonine 159-162 protein phosphatase 2 catalytic subunit alpha Homo sapiens 70-76 18056704-11 2008 Whereas low concentrations of rapamycin (1 ng/ml) inhibited dissociation of PP2Ac after IGF-I stimulation, it required higher concentrations (> or =100 ng/ml) to block EGF-induced dissociation, consistent with the ability for rapamycin to attenuate growth factor-induced activation of p44/42. Sirolimus 30-39 protein phosphatase 2 catalytic subunit alpha Homo sapiens 76-81 18056704-15 2008 This suggests a model in which mTORC1 modulates the phosphorylation of Thr(202) on p44/42 MAPKs through direct or indirect regulation of PP2Ac. Threonine 71-74 protein phosphatase 2 catalytic subunit alpha Homo sapiens 137-142 17540780-11 2007 NaB effects on the NHE3 promoter were significantly attenuated by protein phosphatase (PP)1alpha- and PP2Aalpha-specific siRNA transfection. nab 0-3 protein phosphatase 2 catalytic subunit alpha Homo sapiens 102-111 17274953-2 2007 The catalytic subunit (PP2A(C)) undergoes reversible carboxyl-methylation and -demethylation at its C-terminal leucine residue (Leu309), catalyzed by PP2A-methyltransferase (PMT) and PP2A methylesterase (PME-1), respectively. Leucine 111-118 protein phosphatase 2 catalytic subunit alpha Homo sapiens 23-30 17121919-0 2006 Cantharidin-induced mitotic arrest is associated with the formation of aberrant mitotic spindles and lagging chromosomes resulting, in part, from the suppression of PP2Aalpha. Cantharidin 0-11 protein phosphatase 2 catalytic subunit alpha Homo sapiens 165-174 16682503-6 2006 Furthermore, ceramide- and etoposide-induced PP2A activation involved methylation of PP2A C subunit, which lithium suppressed. Ceramides 13-21 protein phosphatase 2 catalytic subunit alpha Homo sapiens 85-91 16682503-6 2006 Furthermore, ceramide- and etoposide-induced PP2A activation involved methylation of PP2A C subunit, which lithium suppressed. Etoposide 27-36 protein phosphatase 2 catalytic subunit alpha Homo sapiens 85-91 16682503-6 2006 Furthermore, ceramide- and etoposide-induced PP2A activation involved methylation of PP2A C subunit, which lithium suppressed. Lithium 107-114 protein phosphatase 2 catalytic subunit alpha Homo sapiens 85-91 17163575-4 2007 Around 100 proteins, pulled-down by His-tagged PP2Ac, were identified in 2-D gels stained with CBB. coomassie Brilliant Blue 95-98 protein phosphatase 2 catalytic subunit alpha Homo sapiens 47-52 16982626-6 2006 Overexpression of the PP2A catalytic subunit (PP2A/C) suppresses nicotine-stimulated phosphorylation of mu- and m-calpains, which is associated with inhibition of calpain activity, wound healing, cell migration, and invasion. Nicotine 65-73 protein phosphatase 2 catalytic subunit alpha Homo sapiens 22-44 16982626-6 2006 Overexpression of the PP2A catalytic subunit (PP2A/C) suppresses nicotine-stimulated phosphorylation of mu- and m-calpains, which is associated with inhibition of calpain activity, wound healing, cell migration, and invasion. Nicotine 65-73 protein phosphatase 2 catalytic subunit alpha Homo sapiens 46-52 16679323-6 2006 Overexpression of the PP2A catalytic subunit (PP2A/C) suppressed nicotine-stimulated Bax phosphorylation in association with increased apoptotic cell death. Nicotine 65-73 protein phosphatase 2 catalytic subunit alpha Homo sapiens 22-44 16679323-6 2006 Overexpression of the PP2A catalytic subunit (PP2A/C) suppressed nicotine-stimulated Bax phosphorylation in association with increased apoptotic cell death. Nicotine 65-73 protein phosphatase 2 catalytic subunit alpha Homo sapiens 46-52 16180310-10 2005 The low activity of PP2A in mitotic cells was accompanied by a strong reaction of mitotic PP2A C with anti-P-Tyr antibodies in Western blots, which was reversed by treatment of mitotic cells with TPA. Tyrosine 109-112 protein phosphatase 2 catalytic subunit alpha Homo sapiens 90-96 16224536-3 2005 Treatment of SLE T cells with PP2Ac-siRNA decreased the protein levels and activity of PP2Ac in a specific manner and increased the levels of phosphorylated cAMP response element-binding protein and its binding to the IL2 and c-fos promoters, as well as increased activator protein 1 activity, causing normalization of IL-2 production. Cyclic AMP 157-161 protein phosphatase 2 catalytic subunit alpha Homo sapiens 30-35 16206244-0 2006 Interaction of PP2A catalytic subunit with Rb2/p130 is required for all-trans retinoic acid suppression of ovarian carcinoma cell growth. Tretinoin 78-91 protein phosphatase 2 catalytic subunit alpha Homo sapiens 15-37 16180310-10 2005 The low activity of PP2A in mitotic cells was accompanied by a strong reaction of mitotic PP2A C with anti-P-Tyr antibodies in Western blots, which was reversed by treatment of mitotic cells with TPA. Tetradecanoylphorbol Acetate 196-199 protein phosphatase 2 catalytic subunit alpha Homo sapiens 90-96 15530359-3 2004 Despite sharing the fold and two-metal center of human PP2Calpha, the PstPpp catalytic domain binds a third Mn(2+) in a site created by a large shift in a previously unrecognized flap subdomain adjacent to the active site. Manganese(2+) 108-114 protein phosphatase 2 catalytic subunit alpha Homo sapiens 55-64 15848189-4 2005 Here, we demonstrated that PLMA directly binds to the PP2A catalytic subunit (PP2Ac) in cells by using biotinylated PLMA, and the PLMA-binding site was identified as the Cys-269 residue of PP2Ac. Cysteine 170-173 protein phosphatase 2 catalytic subunit alpha Homo sapiens 54-76 15848189-4 2005 Here, we demonstrated that PLMA directly binds to the PP2A catalytic subunit (PP2Ac) in cells by using biotinylated PLMA, and the PLMA-binding site was identified as the Cys-269 residue of PP2Ac. Cysteine 170-173 protein phosphatase 2 catalytic subunit alpha Homo sapiens 78-83 15848189-4 2005 Here, we demonstrated that PLMA directly binds to the PP2A catalytic subunit (PP2Ac) in cells by using biotinylated PLMA, and the PLMA-binding site was identified as the Cys-269 residue of PP2Ac. Cysteine 170-173 protein phosphatase 2 catalytic subunit alpha Homo sapiens 189-194 15848189-5 2005 Moreover, we revealed that the Cys-269 contributes to the potent inhibition of PP2Ac activity by PLMA. Cysteine 31-34 protein phosphatase 2 catalytic subunit alpha Homo sapiens 79-84 15032357-9 2004 RP C18 using methanol-acetic acid (99.5:0.5) as elution solvent provided good recoveries and precision, thus becoming a cheaper but interesting alternative at 0.1-1 ng/ml spiking levels. Methanol 13-21 protein phosphatase 2 catalytic subunit alpha Homo sapiens 0-4 15032357-9 2004 RP C18 using methanol-acetic acid (99.5:0.5) as elution solvent provided good recoveries and precision, thus becoming a cheaper but interesting alternative at 0.1-1 ng/ml spiking levels. Acetic Acid 22-33 protein phosphatase 2 catalytic subunit alpha Homo sapiens 0-4 12369833-4 2002 In contrast, T(220) is uniquely sensitive to dephosphorylation: three major Ser/Thr protein phosphatases, in the order of efficiency PP2B > PP2A(c) > PP1(c), remove this phosphate group first. Phosphates 176-185 protein phosphatase 2 catalytic subunit alpha Homo sapiens 143-150 12963337-10 2003 The interaction of PP2A(C) with alpha4 was unaffected by rapamycin treatment of mammalian cells; however, protein serine/threonine phosphatase inhibitors such as okadaic acid and microcystin-LR disrupted the alpha4/PP2A complex. Okadaic Acid 162-174 protein phosphatase 2 catalytic subunit alpha Homo sapiens 19-26 12963337-10 2003 The interaction of PP2A(C) with alpha4 was unaffected by rapamycin treatment of mammalian cells; however, protein serine/threonine phosphatase inhibitors such as okadaic acid and microcystin-LR disrupted the alpha4/PP2A complex. cyanoginosin LR 179-193 protein phosphatase 2 catalytic subunit alpha Homo sapiens 19-26 12534451-7 2002 In the control subjects, the insulin-mediated decrease in PP2A-C alpha correlated with the insulin-mediated increase in glucose disposal, glucose oxidation, nonoxidative glucose metabolism (all P < 0.05) and decrease in lipid oxidation (P < 0.01). Glucose 120-127 protein phosphatase 2 catalytic subunit alpha Homo sapiens 58-64 12534451-7 2002 In the control subjects, the insulin-mediated decrease in PP2A-C alpha correlated with the insulin-mediated increase in glucose disposal, glucose oxidation, nonoxidative glucose metabolism (all P < 0.05) and decrease in lipid oxidation (P < 0.01). Glucose 138-145 protein phosphatase 2 catalytic subunit alpha Homo sapiens 58-64 12534451-9 2002 CONCLUSIONS: Down-regulation of PP2A-C alpha expression by insulin in skeletal muscle seems to be associated with a normal insulin action on glucose storage, glucose and lipid oxidation. Glucose 141-148 protein phosphatase 2 catalytic subunit alpha Homo sapiens 32-38 12534451-9 2002 CONCLUSIONS: Down-regulation of PP2A-C alpha expression by insulin in skeletal muscle seems to be associated with a normal insulin action on glucose storage, glucose and lipid oxidation. Glucose 158-165 protein phosphatase 2 catalytic subunit alpha Homo sapiens 32-38 14657198-3 2004 D-erythro-C(6) ceramide activated the catalytic subunit of PP2A (PP2Ac) approximately 3-fold in a stereospecific manner. d-erythro-c(6) ceramide 0-23 protein phosphatase 2 catalytic subunit alpha Homo sapiens 59-63 14657198-3 2004 D-erythro-C(6) ceramide activated the catalytic subunit of PP2A (PP2Ac) approximately 3-fold in a stereospecific manner. d-erythro-c(6) ceramide 0-23 protein phosphatase 2 catalytic subunit alpha Homo sapiens 65-70 14657198-4 2004 In contrast, saturation of the 4-5 double bond, producing D-erythro-dihydro C(6) ceramide, inhibited PP2Ac (IC(50) = 8.5 microM). d-erythro-dihydro c(6) ceramide 58-89 protein phosphatase 2 catalytic subunit alpha Homo sapiens 101-106 14657198-6 2004 Modification of the sphingoid chain also abolished the ability of ceramide to activate PP2Ac. Sphingosine 20-29 protein phosphatase 2 catalytic subunit alpha Homo sapiens 87-92 14657198-6 2004 Modification of the sphingoid chain also abolished the ability of ceramide to activate PP2Ac. Ceramides 66-74 protein phosphatase 2 catalytic subunit alpha Homo sapiens 87-92 11591705-7 2001 Okadaic acid, an inhibitor of PP2A at lower concentration, increased the basal phosphorylation of RelA in melanocytes and blocked the dephosphorylation of RelA after interleukin-1 stimulation. Okadaic Acid 0-12 protein phosphatase 2 catalytic subunit alpha Homo sapiens 30-34