PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 8809420-7 1996 On all CD14 positive cells free Fc epsilon RI expression was rapidly and completely lost during culture in conventional culture media (IMDM, RPMI) but not in phosphate buffered saline (PBS). rpmi 141-145 Fc epsilon receptor Ia Homo sapiens 32-45 8809420-9 1996 However, both IgE binding and calcium mobilization were restored by treatment of fresh non-atopic monocytes with neuraminidase. Calcium 30-37 neuraminidase 1 Homo sapiens 113-126 8809420-10 1996 Importantly, culturing these monocytes overnight in conventional medium containing 2 micrograms/mL IgE induced a cycloheximide insensitive accumulation of IgE bound to Fc epsilon RI and, in addition, led to cell activation. Cycloheximide 113-126 immunoglobulin heavy constant epsilon Homo sapiens 99-102