PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3805049-1 1987 cDNA clones for the P-450(M-1) mRNA, which exhibits a male-specific expression in rat livers, were isolated by using synthetic oligonucleotides as the probes. Oligonucleotides 127-143 cytochrome P450, subfamily 2, polypeptide 11 Rattus norvegicus 20-29 3805049-2 1987 Sequence analysis of the cDNAs showed that P-450(M-1) mRNA contains 1,853 nucleotides in addition to a poly(A) chain, and a single open reading frame of 1,500 nucleotides encodes a polypeptide of 500 amino acids with a Mr = 57,187. Poly A 103-110 cytochrome P450, subfamily 2, polypeptide 11 Rattus norvegicus 43-52 3805049-3 1987 The predicted NH2-terminal sequence of 30 amino acids agrees well with that of the purified protein determined by Edman degradation, and the predicted primary structure included all the partial sequences of six internal peptides of P-450(M-1) obtained by the proteolytic digestion and a conserved amino acid sequence containing a putative heme-binding cysteine, proximate to the COOH terminus of the molecules. Heme 339-343 cytochrome P450, subfamily 2, polypeptide 11 Rattus norvegicus 232-241 3805049-3 1987 The predicted NH2-terminal sequence of 30 amino acids agrees well with that of the purified protein determined by Edman degradation, and the predicted primary structure included all the partial sequences of six internal peptides of P-450(M-1) obtained by the proteolytic digestion and a conserved amino acid sequence containing a putative heme-binding cysteine, proximate to the COOH terminus of the molecules. Cysteine 352-360 cytochrome P450, subfamily 2, polypeptide 11 Rattus norvegicus 232-241 3805049-14 1987 (1984) Biochemistry 23, 4598-4603) (71%), while its sequence similarity with 3-methylcholanthrene-inducible P-450c and P-450d is rather low. Methylcholanthrene 77-97 cytochrome P450, family 1, subfamily a, polypeptide 1 Rattus norvegicus 108-125 3805049-15 1987 Consequently, P-450(M-1) could be structurally classified into the phenobarbital-inducible type of P-450 gene family. Phenobarbital 67-80 cytochrome P450, subfamily 2, polypeptide 11 Rattus norvegicus 14-23 3805049-16 1987 RNA blot analysis using a synthetic oligonucleotide specific for P-450(M-1) revealed that P-450(M-1) mRNA was expressed exclusively in the livers of mature male rats in a sex-specific manner, but not in other tissues so far examined. Oligonucleotides 36-51 cytochrome P450, subfamily 2, polypeptide 11 Rattus norvegicus 90-99