PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 3022802-0 1986 N-ethylmaleimide uncouples the glucagon receptor from the regulatory component of adenylyl cyclase. Ethylmaleimide 0-16 glucagon receptor Mus musculus 31-48 3022802-4 1986 Solubilized hormone-receptor complexes were fractionated as high molecular weight (Kav = 0.16) and low molecular weight (Kav = 0.46) species by gel filtration chromatography; NEM or guanosine 5"-triphosphate (GTP) diminished the fraction of high molecular weight complexes, suggesting that NEM uncouples glucagon receptor-N-protein complexes. Guanosine Triphosphate 182-207 glucagon receptor Mus musculus 304-321 3022802-7 1986 These data suggest that a sensitive thiol group that affects hormone binding resides in the glucagon receptor, which may be a transmembrane protein. Sulfhydryl Compounds 36-41 glucagon receptor Mus musculus 92-109 3022802-9 1986 Stimulation of adenylyl cyclase with fluoride, guanylyl 5"-imidodiphosphate, glucagon, or isoproterenol was observed after fusion of cyc- S49 cells [which lack the stimulatory, guanine nucleotide binding, regulatory protein of adenylyl cyclase (Ns)] with liver membranes alkylated with 1.5 mM NEM. Fluorides 37-45 peptidylprolyl isomerase A, pseudogene 1 Mus musculus 24-27 3022802-9 1986 Stimulation of adenylyl cyclase with fluoride, guanylyl 5"-imidodiphosphate, glucagon, or isoproterenol was observed after fusion of cyc- S49 cells [which lack the stimulatory, guanine nucleotide binding, regulatory protein of adenylyl cyclase (Ns)] with liver membranes alkylated with 1.5 mM NEM. guanylyl 5"-imidodiphosphate 47-75 peptidylprolyl isomerase A, pseudogene 1 Mus musculus 24-27 3022802-9 1986 Stimulation of adenylyl cyclase with fluoride, guanylyl 5"-imidodiphosphate, glucagon, or isoproterenol was observed after fusion of cyc- S49 cells [which lack the stimulatory, guanine nucleotide binding, regulatory protein of adenylyl cyclase (Ns)] with liver membranes alkylated with 1.5 mM NEM. Glucagon 77-85 peptidylprolyl isomerase A, pseudogene 1 Mus musculus 24-27 3022802-9 1986 Stimulation of adenylyl cyclase with fluoride, guanylyl 5"-imidodiphosphate, glucagon, or isoproterenol was observed after fusion of cyc- S49 cells [which lack the stimulatory, guanine nucleotide binding, regulatory protein of adenylyl cyclase (Ns)] with liver membranes alkylated with 1.5 mM NEM. Isoproterenol 90-103 peptidylprolyl isomerase A, pseudogene 1 Mus musculus 24-27 3022802-9 1986 Stimulation of adenylyl cyclase with fluoride, guanylyl 5"-imidodiphosphate, glucagon, or isoproterenol was observed after fusion of cyc- S49 cells [which lack the stimulatory, guanine nucleotide binding, regulatory protein of adenylyl cyclase (Ns)] with liver membranes alkylated with 1.5 mM NEM. Guanine Nucleotides 177-195 peptidylprolyl isomerase A, pseudogene 1 Mus musculus 24-27