PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25246550-1 2014 GTP hydrolysis by elongation factor Tu (EF-Tu), a translational GTPase that delivers aminoacyl-tRNAs to the ribosome, plays a crucial role in decoding and translational fidelity. Guanosine Triphosphate 0-3 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 18-38 25246550-1 2014 GTP hydrolysis by elongation factor Tu (EF-Tu), a translational GTPase that delivers aminoacyl-tRNAs to the ribosome, plays a crucial role in decoding and translational fidelity. Guanosine Triphosphate 0-3 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 40-45 25246550-3 2014 Here we use mutational analysis in combination with measurements of rate/pH profiles, kinetic solvent isotope effects, and ion dependence of GTP hydrolysis by EF-Tu off and on the ribosome to dissect the reaction mechanism. Guanosine Triphosphate 141-144 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 159-164 25246550-6 2014 In contrast, upon cognate codon recognition, the ribosome induces a rearrangement of EF-Tu that renders GTP hydrolysis sensitive to mutations of Asp21 and His84 and insensitive to K(+) ions. Guanosine Triphosphate 104-107 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 85-90