PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 25122779-5 2014 To test this hypothesis, we generated cell lines for doxycycline-inducible expression of short hairpin RNAs (shRNAs) targeting the PI4P effector, four-phosphate adaptor protein 2 (FAPP2). Doxycycline 53-64 pleckstrin homology domain containing A8 Homo sapiens 146-178 25122779-5 2014 To test this hypothesis, we generated cell lines for doxycycline-inducible expression of short hairpin RNAs (shRNAs) targeting the PI4P effector, four-phosphate adaptor protein 2 (FAPP2). Doxycycline 53-64 pleckstrin homology domain containing A8 Homo sapiens 180-185 25122779-10 2014 We also found that HCV significantly increases the level of some glycosphingolipids, whereas adding these lipids to FAPP2-depleted cells partially rescued replication, further arguing for the importance of glycosphingolipids in HCV RNA synthesis. Glycosphingolipids 206-224 pleckstrin homology domain containing A8 Homo sapiens 116-121 25122779-13 2014 Altogether, our study implies that HCV coopts FAPP2 for virus genome replication via PI4P binding and glycosphingolipid transport to the HCV RC. Glycosphingolipids 102-119 pleckstrin homology domain containing A8 Homo sapiens 46-51