PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 23077721-6 2012 We established that the addition of choline to these cells, at concentrations appropriate for high-affinity choline transport at presynaptic terminals, generates a hemicholinium-3 (HC-3)-sensitive, membrane depolarization that can be used for the screening of CHT inhibitors and activators. Choline 36-43 solute carrier family 5 member 7 Homo sapiens 260-263 23077721-6 2012 We established that the addition of choline to these cells, at concentrations appropriate for high-affinity choline transport at presynaptic terminals, generates a hemicholinium-3 (HC-3)-sensitive, membrane depolarization that can be used for the screening of CHT inhibitors and activators. Choline 108-115 solute carrier family 5 member 7 Homo sapiens 260-263 23077721-6 2012 We established that the addition of choline to these cells, at concentrations appropriate for high-affinity choline transport at presynaptic terminals, generates a hemicholinium-3 (HC-3)-sensitive, membrane depolarization that can be used for the screening of CHT inhibitors and activators. Hemicholinium 3 164-179 solute carrier family 5 member 7 Homo sapiens 260-263 23077721-6 2012 We established that the addition of choline to these cells, at concentrations appropriate for high-affinity choline transport at presynaptic terminals, generates a hemicholinium-3 (HC-3)-sensitive, membrane depolarization that can be used for the screening of CHT inhibitors and activators. Hemicholinium 3 181-186 solute carrier family 5 member 7 Homo sapiens 260-263 23077721-7 2012 Using this assay, we discovered that staurosporine increased CHT LV-AA choline uptake activity, an effect mediated by a decrease in choline K(M) with no change in V(max). Staurosporine 37-50 solute carrier family 5 member 7 Homo sapiens 61-64 23077721-7 2012 Using this assay, we discovered that staurosporine increased CHT LV-AA choline uptake activity, an effect mediated by a decrease in choline K(M) with no change in V(max). Choline 71-78 solute carrier family 5 member 7 Homo sapiens 61-64 23077721-7 2012 Using this assay, we discovered that staurosporine increased CHT LV-AA choline uptake activity, an effect mediated by a decrease in choline K(M) with no change in V(max). Choline 132-139 solute carrier family 5 member 7 Homo sapiens 61-64 23077721-9 2012 Surprisingly, staurosporine reduced choline-induced membrane depolarization, suggesting that increased substrate coupling to ion gradients, arising at the expense of nonstoichiometric ion flow, accompanies a shift of CHT to a higher-affinity state. Staurosporine 14-27 solute carrier family 5 member 7 Homo sapiens 217-220 23077721-9 2012 Surprisingly, staurosporine reduced choline-induced membrane depolarization, suggesting that increased substrate coupling to ion gradients, arising at the expense of nonstoichiometric ion flow, accompanies a shift of CHT to a higher-affinity state. Choline 36-43 solute carrier family 5 member 7 Homo sapiens 217-220