PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 1925827-1 1991 Human erythrocyte porphobilinogen deaminase was isolated using ammonium sulphate fractionation and heat treatment, Sephadex G-25 and G-100 chromatography, di-ethylamino-ethyl anion-exchange chromatography, chromatofocusing over a pH gradient of 7-4 and, finally, hydrophobic interaction chromatography on a phenyl-Sepharose column. sephadex 115-128 hydroxymethylbilane synthase Homo sapiens 18-43