PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
18452279-1	2008	The serine hydrolase monoacylglycerol lipase (MGL) modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors.	glyceryl 2-arachidonate	111-133	monoglyceride lipase	Homo sapiens	21-44	
18452279-1	2008	The serine hydrolase monoacylglycerol lipase (MGL) modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors.	glyceryl 2-arachidonate	111-133	monoglyceride lipase	Homo sapiens	46-49	
18452279-1	2008	The serine hydrolase monoacylglycerol lipase (MGL) modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors.	glyceryl 2-arachidonate	111-133	cannabinoid receptor 1	Homo sapiens	182-185	
18452279-1	2008	The serine hydrolase monoacylglycerol lipase (MGL) modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors.	glyceryl 2-arachidonate	111-133	cannabinoid receptor 2	Homo sapiens	201-204	
18452279-1	2008	The serine hydrolase monoacylglycerol lipase (MGL) modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors.	glyceryl 2-arachidonate	135-139	monoglyceride lipase	Homo sapiens	21-44	
18452279-1	2008	The serine hydrolase monoacylglycerol lipase (MGL) modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors.	glyceryl 2-arachidonate	135-139	monoglyceride lipase	Homo sapiens	46-49	
18452279-1	2008	The serine hydrolase monoacylglycerol lipase (MGL) modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors.	glyceryl 2-arachidonate	135-139	cannabinoid receptor 1	Homo sapiens	182-185	
18452279-1	2008	The serine hydrolase monoacylglycerol lipase (MGL) modulates endocannabinoid signaling in vivo by inactivating 2-arachidonoylglycerol (2-AG), the main endogenous agonist for central CB1 and peripheral CB2 cannabinoid receptors.	glyceryl 2-arachidonate	135-139	cannabinoid receptor 2	Homo sapiens	201-204	
18452279-2	2008	To characterize this key endocannabinoid enzyme by mass spectrometry-based proteomics, we first overexpressed recombinant hexa-histidine-tagged human MGL (hMGL) in Escherichia coli and purified it in a single chromatographic step with high yield (approximately 30 mg/L).	His-His-His-His-His-His	122-136	monoglyceride lipase	Homo sapiens	150-153	
18452279-2	2008	To characterize this key endocannabinoid enzyme by mass spectrometry-based proteomics, we first overexpressed recombinant hexa-histidine-tagged human MGL (hMGL) in Escherichia coli and purified it in a single chromatographic step with high yield (approximately 30 mg/L).	His-His-His-His-His-His	122-136	monoglyceride lipase	Homo sapiens	155-159	
18452279-3	2008	With 2-AG as substrate, hMGL displayed an apparent V max of 25 micromol/(microg min) and K m of 19.7 microM, an affinity for 2-AG similar to that of native rat-brain MGL (rMGL) (Km=33.6 microM).	glyceryl 2-arachidonate	5-9	monoglyceride lipase	Homo sapiens	24-28	
18452279-3	2008	With 2-AG as substrate, hMGL displayed an apparent V max of 25 micromol/(microg min) and K m of 19.7 microM, an affinity for 2-AG similar to that of native rat-brain MGL (rMGL) (Km=33.6 microM).	glyceryl 2-arachidonate	5-9	monoglyceride lipase	Rattus norvegicus	25-28	
18452279-3	2008	With 2-AG as substrate, hMGL displayed an apparent V max of 25 micromol/(microg min) and K m of 19.7 microM, an affinity for 2-AG similar to that of native rat-brain MGL (rMGL) (Km=33.6 microM).	glyceryl 2-arachidonate	125-129	monoglyceride lipase	Homo sapiens	24-28	
18452279-3	2008	With 2-AG as substrate, hMGL displayed an apparent V max of 25 micromol/(microg min) and K m of 19.7 microM, an affinity for 2-AG similar to that of native rat-brain MGL (rMGL) (Km=33.6 microM).	glyceryl 2-arachidonate	125-129	monoglyceride lipase	Rattus norvegicus	25-28	
18452279-4	2008	hMGL also demonstrated a comparable affinity (Km approximately 8-9 microM) for the novel fluorogenic substrate, arachidonoyl, 7-hydroxy-6-methoxy-4-methylcoumarin ester (AHMMCE), in a sensitive, high-throughput fluorometric MGL assay.	arachidonoyl	112-124	monoglyceride lipase	Homo sapiens	0-4	
18452279-4	2008	hMGL also demonstrated a comparable affinity (Km approximately 8-9 microM) for the novel fluorogenic substrate, arachidonoyl, 7-hydroxy-6-methoxy-4-methylcoumarin ester (AHMMCE), in a sensitive, high-throughput fluorometric MGL assay.	arachidonoyl	112-124	monoglyceride lipase	Rattus norvegicus	1-4	
18452279-4	2008	hMGL also demonstrated a comparable affinity (Km approximately 8-9 microM) for the novel fluorogenic substrate, arachidonoyl, 7-hydroxy-6-methoxy-4-methylcoumarin ester (AHMMCE), in a sensitive, high-throughput fluorometric MGL assay.	7-hydroxy-6-methoxy-4-methylcoumarin ester	126-168	monoglyceride lipase	Homo sapiens	0-4	
18452279-4	2008	hMGL also demonstrated a comparable affinity (Km approximately 8-9 microM) for the novel fluorogenic substrate, arachidonoyl, 7-hydroxy-6-methoxy-4-methylcoumarin ester (AHMMCE), in a sensitive, high-throughput fluorometric MGL assay.	7-hydroxy-6-methoxy-4-methylcoumarin ester	126-168	monoglyceride lipase	Rattus norvegicus	1-4	
18452279-4	2008	hMGL also demonstrated a comparable affinity (Km approximately 8-9 microM) for the novel fluorogenic substrate, arachidonoyl, 7-hydroxy-6-methoxy-4-methylcoumarin ester (AHMMCE), in a sensitive, high-throughput fluorometric MGL assay.	ahmmce	170-176	monoglyceride lipase	Homo sapiens	0-4	
18452279-4	2008	hMGL also demonstrated a comparable affinity (Km approximately 8-9 microM) for the novel fluorogenic substrate, arachidonoyl, 7-hydroxy-6-methoxy-4-methylcoumarin ester (AHMMCE), in a sensitive, high-throughput fluorometric MGL assay.	ahmmce	170-176	monoglyceride lipase	Rattus norvegicus	1-4	
18452279-6	2008	After in-solution tryptic digestion, hMGL full proteomic characterization was carried out, which showed (1) an absence of intramolecular disulfide bridges in the functional, recombinant enzyme and (2) the post-translational removal of the enzyme"s N-terminal methionine.	Methionine	259-269	monoglyceride lipase	Homo sapiens	37-41