PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
18331839-4	2008	We show that thrombin can be used for the formation of N-terminal Cys, providing another choice of reagents for expressed protein ligation.	Cysteine	66-69	coagulation factor II, thrombin	Homo sapiens	13-21	
18331839-5	2008	Proteins with N-terminal Cys can be obtained by the convenient modification of vectors with the putative thrombin cleavage site LVPRG to LVPRC.	Cysteine	25-28	coagulation factor II, thrombin	Homo sapiens	105-113	
18331839-7	2008	The use of thrombin protease to generate N-terminal Cys overcomes some of the limitations of existing methods, making it generally useful for expressed protein ligation and other biotechnological applications.	Cysteine	52-55	coagulation factor II, thrombin	Homo sapiens	11-19