PMID-sentid Pub_year Sent_text comp_official_name comp_offsetprotein_name organism prot_offset 16377633-2 2006 From pharmacological analysis of the recombinant T-type channels, low concentrations (<50 microM) of nickel were found to selectively block the Ca(v)3.2 over the other isoforms. Nickel 104-110 immunoglobulin lambda variable 7-43 Homo sapiens 147-155 16377633-3 2006 To date, however, the structural element(s) responsible for the nickel block on the Ca(v)3.2 T-type Ca2+ channel remain unknown. Nickel 64-70 immunoglobulin lambda variable 7-43 Homo sapiens 84-92 16377633-4 2006 Thus, we constructed chimeric channels between the nickel-sensitive Ca(v)3.2 and the nickel-insensitive Ca(v)3.1 to localize the region interacting with nickel. Nickel 51-57 immunoglobulin lambda variable 7-43 Homo sapiens 68-76 16377633-5 2006 Systematic assaying of serial chimeras suggests that the region preceding domain I S4 of Ca(v)3.2 contributes to nickel block. Nickel 113-119 immunoglobulin lambda variable 7-43 Homo sapiens 89-97 16377633-6 2006 Point mutations of potential nickel-interacting sites revealed that H191Q in the S3-S4 loop of domain I significantly attenuated the nickel block of Ca(v)3.2, mimicking the nickel-insensitive blocking potency of Ca(v)3.1. Nickel 29-35 immunoglobulin lambda variable 7-43 Homo sapiens 149-157 16377633-6 2006 Point mutations of potential nickel-interacting sites revealed that H191Q in the S3-S4 loop of domain I significantly attenuated the nickel block of Ca(v)3.2, mimicking the nickel-insensitive blocking potency of Ca(v)3.1. Nickel 133-139 immunoglobulin lambda variable 7-43 Homo sapiens 149-157 16377633-6 2006 Point mutations of potential nickel-interacting sites revealed that H191Q in the S3-S4 loop of domain I significantly attenuated the nickel block of Ca(v)3.2, mimicking the nickel-insensitive blocking potency of Ca(v)3.1. Nickel 133-139 immunoglobulin lambda variable 7-43 Homo sapiens 149-157 16377633-7 2006 These findings indicate that His-191 in the S3-S4 loop is a critical residue conferring nickel block to Ca(v)3.2 and reveal a novel role for the S3-S4 loop to control ion permeation through T-type Ca2+ channels. Nickel 88-94 immunoglobulin lambda variable 7-43 Homo sapiens 104-112