PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
11490098-3	2001	One of the post-receptor events shortly after the addition of TNF is the generation of the superoxide anion (O2-*).	Superoxides	91-107	tumor necrosis factor	Mus musculus	62-65	
11490098-3	2001	One of the post-receptor events shortly after the addition of TNF is the generation of the superoxide anion (O2-*).	Superoxides	109-113	tumor necrosis factor	Mus musculus	62-65	
11490098-4	2001	In the present study, we attempted to examine the role of O2-* in the regulation of mitochondrial membrane potential (Delta(Psi)m) and the release of cytochrome c (cyto c) in L929 cells after stimulation with TNF.	Superoxides	58-60	tumor necrosis factor	Mus musculus	209-212	
11490098-5	2001	Challenge of cells with TNF (50 ng/ml) resulted in an early (30 min after the addition of TNF) increase in the production of O2-*.	Superoxides	125-127	tumor necrosis factor	Mus musculus	24-27	
11490098-5	2001	Challenge of cells with TNF (50 ng/ml) resulted in an early (30 min after the addition of TNF) increase in the production of O2-*.	Superoxides	125-127	tumor necrosis factor	Mus musculus	90-93	
11490098-6	2001	The use of mitochondrial electron transport chain inhibitors such as antimycin A and rotenone could, respectively, potentiate or suppress the TNF-mediated release of O2-* and cytotoxicity.	Superoxides	166-168	tumor necrosis factor	Mus musculus	142-145	
11490098-7	2001	TNF also induced a late (>3 h after the addition of TNF) depolarization in the Delta(Psi)m. Reduction in the release of O2-* by rotenone (50 microM) or thenoyltrifluoroacetone (250 microM) suppressed both the TNF-mediated Delta(Psi)m depolarization and cyto c release.	Superoxides	123-125	tumor necrosis factor	Mus musculus	0-3	
11490098-7	2001	TNF also induced a late (>3 h after the addition of TNF) depolarization in the Delta(Psi)m. Reduction in the release of O2-* by rotenone (50 microM) or thenoyltrifluoroacetone (250 microM) suppressed both the TNF-mediated Delta(Psi)m depolarization and cyto c release.	Superoxides	123-125	tumor necrosis factor	Mus musculus	55-58	
11490098-7	2001	TNF also induced a late (>3 h after the addition of TNF) depolarization in the Delta(Psi)m. Reduction in the release of O2-* by rotenone (50 microM) or thenoyltrifluoroacetone (250 microM) suppressed both the TNF-mediated Delta(Psi)m depolarization and cyto c release.	Superoxides	123-125	tumor necrosis factor	Mus musculus	55-58	
11490098-8	2001	However, increase in the production of O2-* by antimycin A (25 microM) only slightly enhanced the TNF effect in altering the Delta(Psi)m and the release of cyto c. Treating cells with antimycin A alone could not induce a reduction in Delta(Psi)m nor a release of cyto c. Taken together, our results indicate that TNF induced damage in mitochondria in L929 cells.	Superoxides	39-41	tumor necrosis factor	Mus musculus	98-101	
11490098-8	2001	However, increase in the production of O2-* by antimycin A (25 microM) only slightly enhanced the TNF effect in altering the Delta(Psi)m and the release of cyto c. Treating cells with antimycin A alone could not induce a reduction in Delta(Psi)m nor a release of cyto c. Taken together, our results indicate that TNF induced damage in mitochondria in L929 cells.	Superoxides	39-41	tumor necrosis factor	Mus musculus	313-316	
11490098-9	2001	Our data also show that an increase in the production of O2-* was important in the TNF cytotoxicity, but was not sufficient to mimic the action of TNF.	Superoxides	57-59	tumor necrosis factor	Mus musculus	83-86