PMID-sentid	Pub_year	Sent_text		comp_official_name	comp_offsetprotein_name	organism	prot_offset
10965905-5	2000	In both cells, GH-induced MAP kinase activation was markedly blunted by the MEK1 inhibitor, PD98059, but not by the PKC inhibitor, GF109203X.	2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one	92-99	mitogen activated protein kinase kinase 1	Rattus norvegicus	76-80	
10965905-6	2000	Interestingly, pretreatment with the PI3K inhibitor, wortmannin (EC50 approximately 10 nM), significantly reduced GH-induced MAP kinase activation in both 32D-rbGHR and 32D-rbGHR-IRS-1 cells.	Wortmannin	53-63	insulin receptor substrate 1	Rattus norvegicus	169-184	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	insulin receptor substrate 1	Rattus norvegicus	68-73	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	AKT serine/threonine kinase 1	Rattus norvegicus	156-159	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	mitogen activated protein kinase kinase 1	Rattus norvegicus	164-168	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	growth hormone receptor	Rattus norvegicus	254-257	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	Janus kinase 2	Rattus norvegicus	259-263	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	protein tyrosine phosphatase, non-receptor type 11	Rattus norvegicus	265-270	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	AKT serine/threonine kinase 1	Rattus norvegicus	277-280	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	Raf-1 proto-oncogene, serine/threonine kinase	Rattus norvegicus	287-292	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	mitogen activated protein kinase kinase 1	Rattus norvegicus	294-298	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	mitogen activated protein kinase 3	Rattus norvegicus	300-304	
10965905-7	2000	This same pattern in both cells of IRS-1-dependent augmentation and IRS-1-independent wortmannin sensitivity was also observed for GH-induced activation of Akt and MEK1 (using state-specific antibody blotting for both), despite the lack of difference in GHR, JAK2, SHP-2, p85, Akt, Ras, Raf-1, MEK1, ERK1, or ERK2 abundance between the two cells.	Wortmannin	86-96	mitogen activated protein kinase 1	Rattus norvegicus	309-313	
10965905-8	2000	A different PI3K inhibitor, LY294002 (50 microM), substantially inhibited (roughly 72%) GH-induced MAP kinase activation in 32D-rbGHR-IRS-1 cells, but only marginally (and statistically insignificantly) inhibited GH-induced MAP kinase activation in 32D-rbGHR cells.	2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one	28-36	isoleucine-tRNA synthetase	Mus musculus	134-137	
10965905-9	2000	Because GH-induced Akt activation was completely inhibited in both cells by the same concentration of LY294002, these findings indicate that the wortmannin sensitivity of both the IRS-1-independent and -dependent GH-induced MAP kinase activation may reflect the activity of another wortmannin-sensitive target(s) in addition to PI3K in mediation of GH-induced MAP kinase activation in these cells.	2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one	102-110	AKT serine/threonine kinase 1	Rattus norvegicus	19-22	
10965905-9	2000	Because GH-induced Akt activation was completely inhibited in both cells by the same concentration of LY294002, these findings indicate that the wortmannin sensitivity of both the IRS-1-independent and -dependent GH-induced MAP kinase activation may reflect the activity of another wortmannin-sensitive target(s) in addition to PI3K in mediation of GH-induced MAP kinase activation in these cells.	2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one	102-110	insulin receptor substrate 1	Rattus norvegicus	180-185	
10965905-9	2000	Because GH-induced Akt activation was completely inhibited in both cells by the same concentration of LY294002, these findings indicate that the wortmannin sensitivity of both the IRS-1-independent and -dependent GH-induced MAP kinase activation may reflect the activity of another wortmannin-sensitive target(s) in addition to PI3K in mediation of GH-induced MAP kinase activation in these cells.	Wortmannin	145-155	AKT serine/threonine kinase 1	Rattus norvegicus	19-22	
10965905-9	2000	Because GH-induced Akt activation was completely inhibited in both cells by the same concentration of LY294002, these findings indicate that the wortmannin sensitivity of both the IRS-1-independent and -dependent GH-induced MAP kinase activation may reflect the activity of another wortmannin-sensitive target(s) in addition to PI3K in mediation of GH-induced MAP kinase activation in these cells.	Wortmannin	145-155	insulin receptor substrate 1	Rattus norvegicus	180-185	
10965905-10	2000	Notably, GH-induced STAT5 tyrosine phosphorylation, unlike Akt or MAPK activation, did not differ between the cells.	Tyrosine	26-34	signal transducer and activator of transcription 5A	Rattus norvegicus	20-25	
10965905-12	2000	These data indicate that while GH induces tyrosine phosphorylation of STAT5 and activation of the Ras/Raf/MEK1/MAPK and PI3K pathways, IRS-1 expression augments the latter two more than the former.	Tyrosine	42-50	signal transducer and activator of transcription 5A	Rattus norvegicus	70-75