PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
15769087-4	2005	An 18 mer amino-derivitized MORF was made bivalent by dimerizing with disuccinimidyl suberate (DSS) in 1-methyl-2-pyrrolidinone (NMP) with N,N-diisopropylethylamine (DIEA) followed by purification by ion exchange chromatography.	disuccinimidyl	70-84	lysine acetyltransferase 6B	Homo sapiens	28-32
25350700-9	2014	Next, we generated cross-linked data from an ssDNA binding protein, Rim1with a specific cross-linker disuccinimidyl suberate, and a semi-specific cross-linker NHS-Diazirine, followed by analysis of the cross-linked products by nanoLC-LTQ-Orbitrap mass spectrometry.	disuccinimidyl	101-115	regulating synaptic membrane exocytosis 1	Homo sapiens	68-72
23613850-7	2013	Akt antibody was first immobilized on protein A/G beads using disuccinimidyl suberate and allowed to bind to cellular Akt along with its interacting proteins.	disuccinimidyl	62-76	AKT serine/threonine kinase 1	Homo sapiens	0-3
15695509-10	2005	Cross-linking studies of the meprin B dimer with the amine-reactive cross-linker disuccinimidyl suberate revealed inter- and intradomain contacts within the protein, including prosequence-prosequence, protease-TRAF, protease-epidermal growth factor, and TRAF-TRAF interactions.	disuccinimidyl	81-95	meprin A subunit beta	Homo sapiens	29-37
15118992-2	2004	In order to mimic Abetaaggregates, Abeta-(1-40) was crosslinked to bovine serum albumin (BSA) with disuccinimidyl suberate according to a previously described procedure.	disuccinimidyl	99-113	albumin	Homo sapiens	74-87
15519239-3	2004	Treatment of intact, living cells with the chemical crosslinker disuccinimidyl suberate (DSS) revealed that DJ-1WT and mutant DJ-1M26I were present as stable homodimers; DJ-1L166P in particular tended to form high-order complexes as well.	disuccinimidyl	64-78	Parkinsonism associated deglycase	Homo sapiens	108-112
15231309-1	2004	Force curves were obtained on the live cell surface using an atomic force microscope mounted with a modified tip with the bifunctional covalent crosslinker, disuccinimidyl suberate, which forms a covalent bond with amino-bearing molecules on the cell surface.	disuccinimidyl	157-171	TOR signaling pathway regulator	Homo sapiens	109-112
12217076-10	2002	In addition, an association of ALB3 with the cpSecY complex was demonstrated directly by cross-linking experiments using the chemical cross-linker disuccinimidyl suberate.	disuccinimidyl	147-161	63 kDa inner membrane family protein	Arabidopsis thaliana	31-35
11356684-9	2001	Cross-linking experiments using disuccinimidyl suberate revealed that in the cell lines expressing G protein alpha(i) and G protein alpha(q), both G proteins coupled to the LHRH receptor.	disuccinimidyl	32-46	gonadotropin releasing hormone 1	Homo sapiens	173-177
12029097-2	2002	(125)I-YQLS or (125)I-YQS was cross-linked to CRFR1 using the chemical cross-linker, disuccinimidyl suberate (DSS), which cross-links the epsilon amino groups of lysine residues that have a molecular distance of 11.4 A.	disuccinimidyl	85-99	corticotropin releasing hormone receptor 1	Homo sapiens	46-51
11087120-7	2000	Chemical cross-linking of the two chimeras suggests that disuccinimidyl suberate interacts with the C-terminal half of ecto-ATPase in a manner that results in an increase of activity for both the ecto-ATPase and the ecto-apyrase/ecto-ATPase chimera.	disuccinimidyl	57-71	ectonucleoside triphosphate diphosphohydrolase 1	Homo sapiens	216-228
18432801-6	2001	This method employs the chemical disuccinimidyl suberate (DSS) to achieve irreversible cross-linking of IL-2 to IL-2R.	disuccinimidyl	33-47	interleukin 2	Homo sapiens	104-108
18432801-6	2001	This method employs the chemical disuccinimidyl suberate (DSS) to achieve irreversible cross-linking of IL-2 to IL-2R.	disuccinimidyl	33-47	interleukin 2 receptor subunit alpha	Homo sapiens	112-117
9571191-6	1998	125I-PCDGF was chemically crosslinked to cell surface receptors on CCL64 cells with disuccinimidyl suberate.	disuccinimidyl	84-98	granulin	Mus musculus	5-10
9762008-7	1998	Affinity cross-linking with [125I]IGF-I using disuccinimidyl suberate and SDS-PAGE under reducing conditions yielded a polypeptide band with apparent Mr 130,000, consistent with the alpha-subunit of the IGF-I receptor.	disuccinimidyl	46-60	insulin like growth factor 1	Homo sapiens	34-39
8639831-7	1996	125I-VIP was covalently cross-linked to human platelet membranes using the homobifunctional reagent disuccinimidyl suberate, followed by polyacrylamide gel electrophoresis and autoradiography.	disuccinimidyl	100-114	vasoactive intestinal peptide	Homo sapiens	5-8
9058190-2	1997	When actin molecules were crosslinked with DSS (disuccinimidyl suberate), the myosin ATPase activity in the presence of the modified actin filaments complexed with both troponin and tropomyosin was only 10% less than that in the case of unmodified actin, and the ATPase activation was independent of calcium ions.	disuccinimidyl	48-62	myosin heavy chain 14	Homo sapiens	78-84
9058190-2	1997	When actin molecules were crosslinked with DSS (disuccinimidyl suberate), the myosin ATPase activity in the presence of the modified actin filaments complexed with both troponin and tropomyosin was only 10% less than that in the case of unmodified actin, and the ATPase activation was independent of calcium ions.	disuccinimidyl	48-62	dynein axonemal heavy chain 8	Homo sapiens	85-91
9058190-2	1997	When actin molecules were crosslinked with DSS (disuccinimidyl suberate), the myosin ATPase activity in the presence of the modified actin filaments complexed with both troponin and tropomyosin was only 10% less than that in the case of unmodified actin, and the ATPase activation was independent of calcium ions.	disuccinimidyl	48-62	dynein axonemal heavy chain 8	Homo sapiens	263-269
8855944-9	1996	At high concentrations, V5D-Gal-1 and N-Gal-1 dimerize and can be covalently cross-linked with disuccinimidyl suberate.	disuccinimidyl	95-109	galectin 1	Homo sapiens	28-33
8855944-9	1996	At high concentrations, V5D-Gal-1 and N-Gal-1 dimerize and can be covalently cross-linked with disuccinimidyl suberate.	disuccinimidyl	95-109	galectin 1	Homo sapiens	40-45
8932339-5	1996	Cross-linking of 125I-labelled TGF-beta1 to melanoma cells, Mel 97, by disuccinimidyl suberate (DSS) demonstrated that CD105 expressed on pigmented cells binds TGF-beta1; the pattern of binding of TGF-beta1 to melanoma cells was found to be similar to that of human umbilical vein endothelial cells.	disuccinimidyl	71-85	transforming growth factor beta 1	Homo sapiens	31-40
8077280-6	1994	This product was also detected following cell surface labeling with 125I, and by cross-linking 125I-EGF to intact cells with disuccinimidyl suberate.	disuccinimidyl	125-139	epidermal growth factor	Homo sapiens	100-103
7529752-6	1995	In conjunction with the chemical cross-linking agent disuccinimidyl suberate, 125I-VIP specifically labeled a 63-kd protein in membranes from ciliary processes.	disuccinimidyl	53-67	VIP peptides	Oryctolagus cuniculus	83-86
8702527-4	1996	No increase in high affinity insulin binding sites was observed on Chinese hamster ovary cells expressing the mutant receptor, and also, affinity cross-linking of 125I-labeled insulin by disuccinimidyl suberate to these cells failed to label the mutant alpha subunit.	disuccinimidyl	187-201	insulin	Homo sapiens	176-183
8702527-6	1996	On solubilization of the mutant insulin receptor in Triton X-100-containing buffers, 125I-insulin was efficiently cross-linked to the receptor alpha subunit by disuccinimidyl suberate.	disuccinimidyl	160-174	insulin receptor	Homo sapiens	32-48
8562964-9	1996	Erythropoietin bound to the surface of infected Sf9 cells could be cross-linked to two proteins with molecular masses of 90 and 65 kD using the homobifunctional cross-linker, disuccinimidyl suberate (DSS).	disuccinimidyl	175-189	erythropoietin	Homo sapiens	0-14
7581990-8	1995	Chemical cross-linking studies using disuccinimidyl suberate indicated that the molecular weight of the CRH receptor in the placenta and rat pituitary is 75 kD.	disuccinimidyl	37-51	corticotropin releasing hormone	Rattus norvegicus	104-107
8143746-5	1994	The proteins in the myometrial membrane preparation were cross-linked to 125I-[Sar1, Ile8]angiotensin II (Sarile) with disuccinimidyl suberate.	disuccinimidyl	119-133	angiotensinogen	Homo sapiens	85-104
8048522-5	1994	Affinity cross-linking of cell membranes using disuccinimidyl suberate as a cross-linking agent under reducing conditions revealed a single polypeptide band (relative mol wt 133,000) representing the alpha-subunit of the IGF-I receptor.	disuccinimidyl	47-61	insulin like growth factor 1	Homo sapiens	221-226
8065549-6	1994	Cross-linking of [125I] hCGRP to the brain membrane fractions with disuccinimidyl suberate revealed specific incorporation of [125I] hCGRP to a protein band of approximate molecular weight 52 kDa.	disuccinimidyl	67-81	calcitonin related polypeptide alpha	Homo sapiens	24-29
8065549-6	1994	Cross-linking of [125I] hCGRP to the brain membrane fractions with disuccinimidyl suberate revealed specific incorporation of [125I] hCGRP to a protein band of approximate molecular weight 52 kDa.	disuccinimidyl	67-81	calcitonin related polypeptide alpha	Homo sapiens	133-138
7515840-6	1994	Affinity labeling of these receptors by covalently binding them to 125I-IGF-I with disuccinimidyl suberate and subsequent electrophoretic analysis of the labeled complex revealed that the inhibitory action of TGF-beta 1 (2 ng/ml, 72 h) occurs at the level of a 135 kDa protein which represents the classical form of the binding subunit of the IGF-I receptor.	disuccinimidyl	83-97	insulin like growth factor 1	Homo sapiens	72-77
7515840-6	1994	Affinity labeling of these receptors by covalently binding them to 125I-IGF-I with disuccinimidyl suberate and subsequent electrophoretic analysis of the labeled complex revealed that the inhibitory action of TGF-beta 1 (2 ng/ml, 72 h) occurs at the level of a 135 kDa protein which represents the classical form of the binding subunit of the IGF-I receptor.	disuccinimidyl	83-97	transforming growth factor beta 1	Homo sapiens	209-219
8360677-7	1993	Cross-linking of 125I-ET-1 to retinal particulate preparations with disuccinimidyl suberate resulted in the labeling of two bands with apparent molecular masses of 52 and 34 kDa.	disuccinimidyl	68-82	endothelin 1	Rattus norvegicus	22-26
7545917-6	1994	Cross-linking of 125I-EMAP II-derived peptide (residues 12-20) by disuccinimidyl suberate to human MPs demonstrated a band, approximately 73 kDa, on reduced sodium dodecyl sulfate-polyacrylamide gel electrophoresis.	disuccinimidyl	66-80	aminoacyl tRNA synthetase complex interacting multifunctional protein 1	Homo sapiens	22-29
8243303-4	1993	Cross-linking of [125I]Ang II to BAC in the presence of disuccinimidyl suberate, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, revealed a band of 70,000 +/- 8,000 mol wt (M(r)) under both reducing and nonreducing conditions.	disuccinimidyl	56-70	angiotensinogen	Rattus norvegicus	23-29
7514602-3	1994	Chemical cross-linking studies with disuccinimidyl suberate in the presence of either cyclophilin A, B, or C in complex with cyclosporin A or FK506 binding protein-FK506 result on the other hand in the apparently exclusive and strictly immunosuppressant-dependent formation of covalent immunophilin-calcineurin B subunit products.	disuccinimidyl	36-50	peptidyl-prolyl cis-trans isomerase A	Bos taurus	86-99
8133076-4	1994	Covalent adducts between molecules of TNF and molecules of the binding protein are then produced by crosslinking with disuccinimidyl suberate (DSS).	disuccinimidyl	118-132	tumor necrosis factor	Homo sapiens	38-41
8440709-2	1993	Recombinant human 125I-interleukin-6 (IL-6) was cross-linked with the homobifunctional reagent disuccinimidyl suberate to human hepatoma cells (HepG2).	disuccinimidyl	95-109	interleukin 6	Homo sapiens	18-36
8369420-2	1993	Two modifications were used: (a) covalent attachment of various amounts of myosin subfragment-1 (S1) with the bifunctional reagent disuccinimidyl suberate and (b) copolymerization of unmodified actin monomers with monomers cross-linked internally with 1-ethyl-3-(dimethylaminopropyl)-carbodiimide.	disuccinimidyl	131-145	myosin heavy chain 14	Homo sapiens	75-81
8449938-8	1993	Studies using 125I-Epo and the covalent cross-linking reagent disuccinimidyl suberate provided unambiguous evidence for ternary complexes of 125I-Epo.EpoR.gp55P on surfaces of all factor-independent cells.	disuccinimidyl	62-76	erythropoietin	Homo sapiens	146-149
8449938-8	1993	Studies using 125I-Epo and the covalent cross-linking reagent disuccinimidyl suberate provided unambiguous evidence for ternary complexes of 125I-Epo.EpoR.gp55P on surfaces of all factor-independent cells.	disuccinimidyl	62-76	erythropoietin receptor	Homo sapiens	150-154
8509402-2	1993	The binding of S-100 protein to GFAP was investigated by fluorescence spectroscopy using acrylodan-S-100 protein and cross-linking experiments using the bifunctional cross-linker, disuccinimidyl suberate.	disuccinimidyl	180-194	glial fibrillary acidic protein	Bos taurus	32-36
8440709-2	1993	Recombinant human 125I-interleukin-6 (IL-6) was cross-linked with the homobifunctional reagent disuccinimidyl suberate to human hepatoma cells (HepG2).	disuccinimidyl	95-109	interleukin 6	Homo sapiens	38-42
8382811-5	1993	Cross-linking of 125I-labeled NT to brain membranes using disuccinimidyl suberate was found to specifically label two substances of approximately 120 kDa and approximately 160 kDa, which could represent different binding proteins or complexes.	disuccinimidyl	58-72	neurotensin/neuromedin N	Cavia porcellus	30-32
1358197-6	1992	Wild-type NPR-A was cross-linked with disuccinimidyl suberate to an M(r) > 500,000 species in the absence of ANP, and with ANP, a smaller, M(r) approximately 400,000 receptor trimer cross-linking product was observed, together with the larger, possibly tetrameric complex.	disuccinimidyl	38-52	natriuretic peptide receptor 1	Homo sapiens	10-15
8383439-10	1993	125I-VIP was cross-linked to membranes using disuccinimidyl suberate.	disuccinimidyl	45-59	vasoactive intestinal peptide	Homo sapiens	5-8
1356991-7	1992	Disuccinimidyl suberate cross-linked [125I]ANP to 135-kDa NPR-A on intact cells.	disuccinimidyl	0-14	natriuretic peptide receptor 1	Homo sapiens	58-63
1526996-4	1992	125I-IL2-receptor complexes were solubilized with the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propane-sulfonate, and IL2-binding polypeptides were identified by cross-linking with disuccinimidyl suberate.	disuccinimidyl	191-205	interleukin 2 receptor subunit beta	Homo sapiens	5-17
1526996-4	1992	125I-IL2-receptor complexes were solubilized with the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propane-sulfonate, and IL2-binding polypeptides were identified by cross-linking with disuccinimidyl suberate.	disuccinimidyl	191-205	interleukin 2	Homo sapiens	5-8
1350903-2	1992	In order to elucidate the possible quarternary structure/function relationship of P-glycoprotein, we treated multidrug-resistant human leukemia K562/ADM cells with the crosslinking reagent, disuccinimidyl suberate.	disuccinimidyl	190-204	ATP binding cassette subfamily B member 1	Homo sapiens	82-96
1657987-6	1991	The binding of EGF to EGF-Rx leads to the formation of receptor dimers and higher oligomerization states which are irreversibly captured using the covalent cross-linking agent disuccinimidyl suberate.	disuccinimidyl	176-190	epidermal growth factor	Homo sapiens	15-18
1381409-5	1992	After prior cross-linking with disuccinimidyl suberate, the 150 kDa fractions (IGFBP-3) from non-pregnant and pregnant serum showed a similar pattern on SDS-PAGE (several bands at different molecular masses).	disuccinimidyl	31-45	insulin like growth factor binding protein 3	Homo sapiens	79-86
1657987-6	1991	The binding of EGF to EGF-Rx leads to the formation of receptor dimers and higher oligomerization states which are irreversibly captured using the covalent cross-linking agent disuccinimidyl suberate.	disuccinimidyl	176-190	epidermal growth factor	Homo sapiens	22-25
1719079-8	1991	Lymphocyte CGRP receptor proteins were identified by affinity labeling by using disuccinimidyl suberate to covalently cross-link 125I-[His10]CGRP to its receptor.	disuccinimidyl	80-94	calcitonin-related polypeptide alpha	Rattus norvegicus	11-15
1719079-8	1991	Lymphocyte CGRP receptor proteins were identified by affinity labeling by using disuccinimidyl suberate to covalently cross-link 125I-[His10]CGRP to its receptor.	disuccinimidyl	80-94	calcitonin-related polypeptide alpha	Rattus norvegicus	141-145
1834739-5	1991	The kinetics of association between CD4 or CD8 and CD45 was measured by fluorescence resonance energy transfer and confirmed by immunoprecipitation of dithiobis succinimidylpropionate or disuccinimidyl suberate cross-linked 125I-labeled resting or activated T cells.	disuccinimidyl	187-201	CD4 molecule	Homo sapiens	36-39
1834739-5	1991	The kinetics of association between CD4 or CD8 and CD45 was measured by fluorescence resonance energy transfer and confirmed by immunoprecipitation of dithiobis succinimidylpropionate or disuccinimidyl suberate cross-linked 125I-labeled resting or activated T cells.	disuccinimidyl	187-201	CD8a molecule	Homo sapiens	43-46
1834739-5	1991	The kinetics of association between CD4 or CD8 and CD45 was measured by fluorescence resonance energy transfer and confirmed by immunoprecipitation of dithiobis succinimidylpropionate or disuccinimidyl suberate cross-linked 125I-labeled resting or activated T cells.	disuccinimidyl	187-201	protein tyrosine phosphatase receptor type C	Homo sapiens	51-55
1849486-1	1991	125I-labelled GLP-I(7-36)amide was cross-liked to a specific binding protein in rat lung membranes using disuccinimidyl suberate.	disuccinimidyl	105-119	granzyme B	Rattus norvegicus	14-19
1653602-7	1991	Cross-linking of 125I-hCGRP-I and -II and 125I-hCGRP-I(8-37) to soluble CGRP binding sites using disuccinimidyl suberate revealed three specifically labeled binding proteins with the same Mr.	disuccinimidyl	97-111	calcitonin related polypeptide alpha	Homo sapiens	22-59
1653602-7	1991	Cross-linking of 125I-hCGRP-I and -II and 125I-hCGRP-I(8-37) to soluble CGRP binding sites using disuccinimidyl suberate revealed three specifically labeled binding proteins with the same Mr.	disuccinimidyl	97-111	calcitonin related polypeptide alpha	Homo sapiens	23-27
1714793-5	1991	Partial characterization of the receptor was accomplished by crosslinking 125I-labeled GHRH to thymus cells with disuccinimidyl suberate and polyacrylamide gel electrophoresis.	disuccinimidyl	113-127	growth hormone releasing hormone	Rattus norvegicus	87-91
1660472-4	1991	When the complexes were cross-linked by disuccinimidyl suberate, the 85- and 100-kDa erythropoietin-cross-linked proteins previously described were immunoprecipitated.	disuccinimidyl	40-54	erythropoietin	Mus musculus	85-99
1651930-2	1991	In erythroleukemia cells infected with the polycythemia strain of the Friend virus complex, erythropoietin could be cross-linked mainly to a protein of 63 kDa when using disuccinimidyl suberate.	disuccinimidyl	170-184	erythropoietin	Homo sapiens	92-106
1650327-6	1991	Disuccinimidyl suberate was used to covalently label 125I-transforming growth factor-beta 1 to cell-surface binding sites.	disuccinimidyl	0-14	transforming growth factor beta 1	Homo sapiens	58-91
2072111-4	1991	Moreover, covalent labeling of 125I-Ang II to solubilized receptors with the homo-bifunctional cross-linker disuccinimidyl suberate, followed by gel filtration, revealed one major and one minor binding peak with apparent molecular weights of 64,000 and 115,000, respectively.	disuccinimidyl	108-122	angiotensinogen	Rattus norvegicus	36-42
1645708-8	1991	After cross-linking with disuccinimidyl suberate, electrophoresis of covalent [125I]PYY-receptor complexes in membranes or CHAPS extracts revealed the presence of two bands of Mr 49,000 or 28,000 whose labeling was completely abolished by 1 microM unlabeled PYY.	disuccinimidyl	25-39	peptide YY	Rattus norvegicus	84-87
1847858-10	1991	Affinity labeling of these receptors by covalent attachment to [125I] TGF beta 1 with disuccinimidyl suberate and subsequent electrophoretic analysis of the labeled complexes revealed the specific binding of [125I] TGF beta 1 to three predominant molecules of 260, 130, and 70 kDa.	disuccinimidyl	86-100	transforming growth factor beta-1 proprotein	Canis lupus familiaris	70-80
1847858-10	1991	Affinity labeling of these receptors by covalent attachment to [125I] TGF beta 1 with disuccinimidyl suberate and subsequent electrophoretic analysis of the labeled complexes revealed the specific binding of [125I] TGF beta 1 to three predominant molecules of 260, 130, and 70 kDa.	disuccinimidyl	86-100	transforming growth factor beta-1 proprotein	Canis lupus familiaris	215-225
1847982-8	1991	Affinity cross-linking of 125I-EPO using disuccinimidyl suberate followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions demonstrated that EPO was preferentially cross-linked to a protein of approximately 116 kD.	disuccinimidyl	41-55	erythropoietin	Homo sapiens	31-34
1676485-2	1991	When cell-bound 125I-IFN gamma was cross-linked by disuccinimidyl suberate, the receptor-ligand complex migrated under nonreducing conditions as major bands of 155 kD and 90 kD and a minor band of 65-70 kD.	disuccinimidyl	51-65	interferon gamma	Homo sapiens	21-30
1846877-7	1991	Bound [125I]ANP was covalently cross-linked to the receptor with 5 mM disuccinimidyl suberate.	disuccinimidyl	70-84	natriuretic peptide A	Homo sapiens	12-15
1846294-5	1991	In this report, cross-linking to the receptor through the monofunctional DJ-EP labeled the same 140- and 125-kDa molecular mass bands (100- and 85-kDa proteins) cross-linked with 125I-EP and disuccinimidyl suberate.	disuccinimidyl	191-205	erythropoietin	Homo sapiens	76-78
1847982-8	1991	Affinity cross-linking of 125I-EPO using disuccinimidyl suberate followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions demonstrated that EPO was preferentially cross-linked to a protein of approximately 116 kD.	disuccinimidyl	41-55	erythropoietin	Homo sapiens	179-182
2174333-6	1990	The bioactive binding protein(s) was cross-linked with disuccinimidyl suberate to IGF-I or the recombinant IGF analog [1-27,Gly4,38-70]IGF-I (Analog).	disuccinimidyl	55-69	insulin like growth factor 1	Homo sapiens	82-87
1846107-2	1991	In the present study, the mechanism of this antagonism was examined using a [125I]IGF-II cross-linking assay with disuccinimidyl suberate in cell membranes.	disuccinimidyl	114-128	insulin like growth factor 2	Homo sapiens	82-88
2174333-6	1990	The bioactive binding protein(s) was cross-linked with disuccinimidyl suberate to IGF-I or the recombinant IGF analog [1-27,Gly4,38-70]IGF-I (Analog).	disuccinimidyl	55-69	insulin like growth factor 1	Homo sapiens	135-140
2170560-5	1990	Treatment of surface-bound radiolabeled PSTI with a chemical crosslinker (disuccinimidyl suberate) led to the identification of a membrane polypeptide of Mr 140,000 to which PSTI was crosslinked.	disuccinimidyl	74-88	serine peptidase inhibitor Kazal type 1	Homo sapiens	40-44
2170560-5	1990	Treatment of surface-bound radiolabeled PSTI with a chemical crosslinker (disuccinimidyl suberate) led to the identification of a membrane polypeptide of Mr 140,000 to which PSTI was crosslinked.	disuccinimidyl	74-88	serine peptidase inhibitor Kazal type 1	Homo sapiens	174-178
1706979-4	1990	Chemical cross-linking of 125I-EGF to its receptors using disuccinimidyl suberate (DSS) revealed a radiolabelled band of relative molecular mass 160,000, similar to that identified as the EGF receptor in other cell types.	disuccinimidyl	58-72	epidermal growth factor	Sus scrofa	31-34
2383597-5	1990	The triamcinolone acetonide-induced increase in [125I]EGF binding capacity was dose-dependent between 10(-9) and 10(-6)M, and maximal binding was observed at 10(-6)M. EGF receptors on HSG cells were affinity-labeled with [125I]EGF by use of the cross-linking reagent disuccinimidyl suberate (DSS).	disuccinimidyl	267-281	epidermal growth factor	Homo sapiens	54-57
1703092-1	1990	The subunit interaction of hCG with its rat ovarian LH/CG receptor was studied by cross-linking the solubilized receptor-hormone complex with glutaraldehyde (GA), disuccinimidyl suberate (DSS) or dithiobis(succinimidyl propionate) (DSP) and analyzing the complexes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and autoradiography.	disuccinimidyl	163-177	glycoprotein hormones, alpha polypeptide	Homo sapiens	27-30
2383597-5	1990	The triamcinolone acetonide-induced increase in [125I]EGF binding capacity was dose-dependent between 10(-9) and 10(-6)M, and maximal binding was observed at 10(-6)M. EGF receptors on HSG cells were affinity-labeled with [125I]EGF by use of the cross-linking reagent disuccinimidyl suberate (DSS).	disuccinimidyl	267-281	epidermal growth factor	Homo sapiens	167-170
2383597-5	1990	The triamcinolone acetonide-induced increase in [125I]EGF binding capacity was dose-dependent between 10(-9) and 10(-6)M, and maximal binding was observed at 10(-6)M. EGF receptors on HSG cells were affinity-labeled with [125I]EGF by use of the cross-linking reagent disuccinimidyl suberate (DSS).	disuccinimidyl	267-281	epidermal growth factor	Homo sapiens	167-170
2550441-5	1989	When the purified receptors from these three species were cross-linked to 125I-IGF-II with disuccinimidyl suberate, only receptors isolated from rat membranes were affinity-labeled.	disuccinimidyl	91-105	insulin-like growth factor 2	Rattus norvegicus	79-85
2100269-7	1990	Cross-linking experiments using disuccinimidyl suberate to link the [125I]-EGF to gastric membranes followed by polyacrylamide gel electrophoresis and autoradiography revealed that the major receptor for EGF was a protein of 170 kilodaltons.	disuccinimidyl	32-46	epidermal growth factor like 1	Rattus norvegicus	75-78
2100269-7	1990	Cross-linking experiments using disuccinimidyl suberate to link the [125I]-EGF to gastric membranes followed by polyacrylamide gel electrophoresis and autoradiography revealed that the major receptor for EGF was a protein of 170 kilodaltons.	disuccinimidyl	32-46	epidermal growth factor like 1	Rattus norvegicus	204-207
2557332-4	1989	Cross-linking of 125I-IGF-I to the cell surface with disuccinimidyl suberate followed by analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography reveals a 130-kDa protein (reduced) consistent with the alpha component of a type I receptor and a 38-kDa protein which does not bind insulin, and thus could be another IGF-I cell surface binding protein.	disuccinimidyl	53-67	insulin like growth factor 1	Homo sapiens	22-27
2156850-6	1990	By cross-linking NPY to its receptor with either disuccinimidyl suberate or dithiobis(succinimidyl propionate) and analyzing the resulting complexes on sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by radioautography, we identified two NPY receptor species with molecular sizes of 52-59 kDa and 37-39 kDa.	disuccinimidyl	49-63	neuropeptide Y	Rattus norvegicus	17-20
1690749-4	1990	When [125I]IGF-I or [125I]IGF-II was cross-linked to the 150K BP with disuccinimidyl suberate and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10-15%) and autoradiography, four specifically labeled complexes of 20K, 24K, 33K, and 47K mol wt were identified.	disuccinimidyl	70-84	insulin like growth factor 1	Homo sapiens	11-16
1690749-4	1990	When [125I]IGF-I or [125I]IGF-II was cross-linked to the 150K BP with disuccinimidyl suberate and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (10-15%) and autoradiography, four specifically labeled complexes of 20K, 24K, 33K, and 47K mol wt were identified.	disuccinimidyl	70-84	insulin like growth factor 2	Homo sapiens	26-32
1691502-1	1990	An epidermal growth factor (EGF) receptor monoclonal antibody (mAb), mAb LA22, was used to analyze the covalent coupling of human EGF receptors to mouse EGF by the amine-reactive cross-linking agent disuccinimidyl suberate.	disuccinimidyl	199-213	epidermal growth factor receptor	Homo sapiens	3-41
1691502-1	1990	An epidermal growth factor (EGF) receptor monoclonal antibody (mAb), mAb LA22, was used to analyze the covalent coupling of human EGF receptors to mouse EGF by the amine-reactive cross-linking agent disuccinimidyl suberate.	disuccinimidyl	199-213	epidermal growth factor	Homo sapiens	28-31
1691502-1	1990	An epidermal growth factor (EGF) receptor monoclonal antibody (mAb), mAb LA22, was used to analyze the covalent coupling of human EGF receptors to mouse EGF by the amine-reactive cross-linking agent disuccinimidyl suberate.	disuccinimidyl	199-213	epidermal growth factor	Mus musculus	130-133
1691502-8	1990	These results indicated that disuccinimidyl suberate cross-linked the NH2 group of EGF residue Asn-1 to the human EGF receptor residue Lys-336.	disuccinimidyl	29-43	epidermal growth factor	Homo sapiens	83-86
1691502-8	1990	These results indicated that disuccinimidyl suberate cross-linked the NH2 group of EGF residue Asn-1 to the human EGF receptor residue Lys-336.	disuccinimidyl	29-43	epidermal growth factor	Homo sapiens	114-117
2156441-5	1990	Through the use of 0.5 mM disuccinimidyl suberate, 125I-IGF-II is cross-linked to a 260-kDa protein that is identified as the IGF-II receptor by displacement experiments with unlabeled IGF-II, IGF-I, and insulin and either by immunoprecipitation or by Western blot analysis with mannose 6-phosphate receptor antibodies.	disuccinimidyl	26-40	insulin like growth factor 2	Homo sapiens	56-62
2156441-5	1990	Through the use of 0.5 mM disuccinimidyl suberate, 125I-IGF-II is cross-linked to a 260-kDa protein that is identified as the IGF-II receptor by displacement experiments with unlabeled IGF-II, IGF-I, and insulin and either by immunoprecipitation or by Western blot analysis with mannose 6-phosphate receptor antibodies.	disuccinimidyl	26-40	insulin like growth factor 2 receptor	Homo sapiens	126-141
2156441-5	1990	Through the use of 0.5 mM disuccinimidyl suberate, 125I-IGF-II is cross-linked to a 260-kDa protein that is identified as the IGF-II receptor by displacement experiments with unlabeled IGF-II, IGF-I, and insulin and either by immunoprecipitation or by Western blot analysis with mannose 6-phosphate receptor antibodies.	disuccinimidyl	26-40	insulin like growth factor 2	Homo sapiens	126-132
2156441-5	1990	Through the use of 0.5 mM disuccinimidyl suberate, 125I-IGF-II is cross-linked to a 260-kDa protein that is identified as the IGF-II receptor by displacement experiments with unlabeled IGF-II, IGF-I, and insulin and either by immunoprecipitation or by Western blot analysis with mannose 6-phosphate receptor antibodies.	disuccinimidyl	26-40	insulin like growth factor 1	Homo sapiens	56-61
2136995-5	1990	Conversely, attempts to prevent the dissociation of R[5-HT1A]-G by treatment of CHAPS soluble hippocampal extracts with the cross-linking reagent disuccinimidyl suberate (0.1 mM) resulted in a significant increase (+70%) in [3H]8-OH-DPAT binding activity associated with the appearance of a new sedimenting material with a higher coefficient (16.5 S).	disuccinimidyl	146-160	5-hydroxytryptamine receptor 1A	Rattus norvegicus	54-60
2404777-2	1990	When [125I]mIL-3 was cross-linked to receptor sites on the surfaces of intact B6SUtA1 cells with disuccinimidyl suberate (DSS), sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed the existence of two radiolabeled species with molecular weights of 140 (p140) and 70 (p70) kd (after subtraction of [125I]mIL-3).	disuccinimidyl	97-111	interleukin 3	Mus musculus	11-16
2681215-4	1989	Addition of antiphosphotyrosine antibodies (alpha PTyr Abs) to cell lysates from B6SUtA1 cells, to which 125I-mIL-3 had been disuccinimidyl suberate-cross-linked, resulted in the immunoprecipitation of 125I-mIL-3 complexed to both 70- and 140-kDa proteins.	disuccinimidyl	125-139	interleukin 3	Mus musculus	110-115
2549150-3	1989	Affinity cross-linking of 125I-labelled hCRF to the purified hCRF-BP was performed using disuccinimidyl suberate (1 mmol/l).	disuccinimidyl	89-103	corticotropin releasing hormone binding protein	Homo sapiens	61-68
2543671-2	1989	Using the cross-linking reagents disuccinimidyl suberate and dithiobissuccinimidyl propionate, we show that 125I-Epo can be specifically conjugated in a complex of 224kDa using mouse fetal liver cells, bone marrow cells, and Friend virus-induced splenic erythroblasts as demonstrated by electrophoresis on sodium dodecyl sulfate-polyacrylamide gels under nonreducing conditions.	disuccinimidyl	33-47	erythropoietin	Mus musculus	113-116
2456065-2	1988	When receptor-bound [125I]IGF-II was cross-linked by use of disuccinimidyl suberate, a 240 K-Da protein was radiolabeled.	disuccinimidyl	60-74	insulin-like growth factor 2	Mus musculus	26-32
2536017-3	1989	Chemical cross-linking of Mr 55,000 125I-uPA to the surface of HeLa and U937 cells with disuccinimidyl suberate or with formaldehyde results in the formation of a labeled complex of Mr 100,000, suggesting a Mr of 45,000 +/- 5,000 for the receptor or a subunit thereof.	disuccinimidyl	88-102	plasminogen activator, urokinase	Homo sapiens	41-44
2535812-4	1989	To elucidate PYY receptor structure, we used the cross-linking reagent disuccinimidyl suberate to covalently attach [125I]PYY to its receptors in the hippocampus membrane.	disuccinimidyl	71-85	peptide YY	Homo sapiens	13-16
2535812-4	1989	To elucidate PYY receptor structure, we used the cross-linking reagent disuccinimidyl suberate to covalently attach [125I]PYY to its receptors in the hippocampus membrane.	disuccinimidyl	71-85	peptide YY	Homo sapiens	122-125
2538697-3	1989	[125I]VIP was covalently cross-linked to tracheal membrane proteins using disuccinimidyl suberate.	disuccinimidyl	74-88	vasoactive intestinal peptide	Bos taurus	6-9
2848056-1	1988	A human plasma CRH-binding protein (CRH-BP) was identified and characterized by chemical cross-linking of 125I-Tyr-hCRH to human plasma using disuccinimidyl suberate.	disuccinimidyl	142-156	corticotropin releasing hormone binding protein	Homo sapiens	15-34
2848056-1	1988	A human plasma CRH-binding protein (CRH-BP) was identified and characterized by chemical cross-linking of 125I-Tyr-hCRH to human plasma using disuccinimidyl suberate.	disuccinimidyl	142-156	corticotropin releasing hormone binding protein	Homo sapiens	36-42
2466092-2	1988	Crosslinking of iodinated-recombinant DNA-derived IFN-alpha-Con1, an analog of the known IFN-alpha subtypes, to the cell surface with disuccinimidyl suberate yielded four IFN-receptor complexes of 118, 138, 159, and 260 kD on all cell lines that specifically bind IFN-alpha.	disuccinimidyl	134-148	interferon alpha 1	Homo sapiens	50-59
2466092-2	1988	Crosslinking of iodinated-recombinant DNA-derived IFN-alpha-Con1, an analog of the known IFN-alpha subtypes, to the cell surface with disuccinimidyl suberate yielded four IFN-receptor complexes of 118, 138, 159, and 260 kD on all cell lines that specifically bind IFN-alpha.	disuccinimidyl	134-148	interferon alpha 1	Homo sapiens	89-98
2466092-2	1988	Crosslinking of iodinated-recombinant DNA-derived IFN-alpha-Con1, an analog of the known IFN-alpha subtypes, to the cell surface with disuccinimidyl suberate yielded four IFN-receptor complexes of 118, 138, 159, and 260 kD on all cell lines that specifically bind IFN-alpha.	disuccinimidyl	134-148	interferon alpha 1	Homo sapiens	50-53
2466092-2	1988	Crosslinking of iodinated-recombinant DNA-derived IFN-alpha-Con1, an analog of the known IFN-alpha subtypes, to the cell surface with disuccinimidyl suberate yielded four IFN-receptor complexes of 118, 138, 159, and 260 kD on all cell lines that specifically bind IFN-alpha.	disuccinimidyl	134-148	interferon alpha 1	Homo sapiens	89-98
2460452-10	1988	When cell-bound 125I-IGF-I was cross-linked by use of disuccinimidyl suberate, a 130-kDa protein was radiolabeled.	disuccinimidyl	54-68	insulin-like growth factor 1	Mus musculus	21-26
3263648-4	1988	Analysis of IL-4 by cross-linkage of 125I-labeled IL-4 to IL-4 receptor with disuccinimidyl suberate indicated that the IL-4-IL-4 receptor complex was the same size in the resting and up-regulated cells, implying that the same receptor species found in resting cells was up-regulated in response to IL-4.	disuccinimidyl	77-91	interleukin 4	Homo sapiens	12-16
3263648-4	1988	Analysis of IL-4 by cross-linkage of 125I-labeled IL-4 to IL-4 receptor with disuccinimidyl suberate indicated that the IL-4-IL-4 receptor complex was the same size in the resting and up-regulated cells, implying that the same receptor species found in resting cells was up-regulated in response to IL-4.	disuccinimidyl	77-91	interleukin 4	Homo sapiens	50-54
3263648-4	1988	Analysis of IL-4 by cross-linkage of 125I-labeled IL-4 to IL-4 receptor with disuccinimidyl suberate indicated that the IL-4-IL-4 receptor complex was the same size in the resting and up-regulated cells, implying that the same receptor species found in resting cells was up-regulated in response to IL-4.	disuccinimidyl	77-91	interleukin 4 receptor	Homo sapiens	58-71
3263648-4	1988	Analysis of IL-4 by cross-linkage of 125I-labeled IL-4 to IL-4 receptor with disuccinimidyl suberate indicated that the IL-4-IL-4 receptor complex was the same size in the resting and up-regulated cells, implying that the same receptor species found in resting cells was up-regulated in response to IL-4.	disuccinimidyl	77-91	interleukin 4	Homo sapiens	50-54
3263648-4	1988	Analysis of IL-4 by cross-linkage of 125I-labeled IL-4 to IL-4 receptor with disuccinimidyl suberate indicated that the IL-4-IL-4 receptor complex was the same size in the resting and up-regulated cells, implying that the same receptor species found in resting cells was up-regulated in response to IL-4.	disuccinimidyl	77-91	interleukin 4 receptor	Homo sapiens	125-138
3263648-4	1988	Analysis of IL-4 by cross-linkage of 125I-labeled IL-4 to IL-4 receptor with disuccinimidyl suberate indicated that the IL-4-IL-4 receptor complex was the same size in the resting and up-regulated cells, implying that the same receptor species found in resting cells was up-regulated in response to IL-4.	disuccinimidyl	77-91	interleukin 4	Homo sapiens	50-54
2708454-5	1989	Conditioned medium from these two cell types has similar amounts of unoccupied 28 K protein as evidenced by the ability of 125I-IGF I to specifically bind to and be covalently bound to the protein after treatment with the bifunctional cross-linking reagent disuccinimidyl suberate.	disuccinimidyl	257-271	insulin-like growth factor 1	Mus musculus	128-133
2550026-1	1989	125I-Labelled glucagon-like peptide-1(7-36)amide was cross-linked to a specific binding protein in plasma membranes prepared from RINm5F rat insulinoma-derived cells using disuccinimidyl suberate.	disuccinimidyl	172-186	glucagon	Rattus norvegicus	14-37
2840960-4	1988	Crosslinking of 125I-erythropoietin to its binding sites with disuccinimidyl suberate revealed the existence of a single binding protein with molecular mass of 63 kDa.	disuccinimidyl	62-76	erythropoietin	Mus musculus	21-35
2839512-2	1988	The ligand binding subunits of the corticotropin-releasing factor (CRF) receptors in brain and anterior pituitary of a number of species have been identified by chemical affinity cross-linking using the homobifunctional cross-linking agent disuccinimidyl suberate and 125I-Tyr0-oCRF (ovine CRF).	disuccinimidyl	240-254	corticotropin releasing hormone	Rattus norvegicus	35-65
2836384-4	1988	Chemical cross-linking using disuccinimidyl suberate and the analysis of EGF receptor complexes by Western blotting demonstrated that EGF caused the aggregation of receptors.	disuccinimidyl	29-43	epidermal growth factor	Homo sapiens	134-137
2836166-4	1988	Under reduction conditions, disuccinimidyl suberate cross-linked [125I]iodo-IGF-I to one receptor complex with an Mr of 125,000.	disuccinimidyl	28-42	insulin like growth factor 1	Bos taurus	76-81
2844145-10	1988	When disuccinimidyl suberate was used to covalently couple 125I-GRP to the cells, two major radiolabelled complexes were detected with molecular masses of approx.	disuccinimidyl	5-19	gastrin releasing peptide	Mus musculus	64-67
2844304-6	1988	More precise identification of protein components of the VIP receptor resulted from covalent cross-linking on intact HT 29 cells by four bifunctional reagents: dithiobis-(succinimidyl propionate) and its non-cleavable analog disuccinimidyl suberate, the photoactivable azido phenyl glyoxal and dimethylpimelimidate.	disuccinimidyl	225-239	vasoactive intestinal peptide	Homo sapiens	57-60
2968745-5	1988	Cross-linking of 125I-IGF-I to the receptor with disuccinimidyl suberate, followed by SDS-polyacrylamide gel electrophoresis under reducing conditions showed an IGF-I binding protein with a molecular weight of 146,000 Dalton.	disuccinimidyl	49-63	IGFI	Bos taurus	22-27
2969326-4	1988	[125I]IGF-II was covalently cross-linked with disuccinimidyl suberate to its receptor in rat renal glomeruli and a specific high mol wt (255,000) band could be identified on autoradiograms of dodecyl sulfate-polyacrylamide gels.	disuccinimidyl	46-60	insulin-like growth factor 2	Rattus norvegicus	6-12
2826160-3	1987	Cross-linking studies with disuccinimidyl suberate and N-hydroxysuccinimidyl-4-azidobenzoate using membrane-solubilised, partially purified and CGRP-affinity-purified preparations, show a number of 125I-alpha-CGRP binding subunit(s) of Mr 62,000-68,000.	disuccinimidyl	27-41	calcitonin related polypeptide alpha	Homo sapiens	144-148
2466913-2	1988	When [32P]HuIFN-gamma was bound and crosslinked to IFN-gamma the receptor of human cells with a bifunctional crosslinker disuccinimidyl suberate (DSS), a single diffused 32P-labeled band corresponding to the IFN-gamma.receptor complex was visualized by SDS-polyacrylamide gel electrophoresis and autoradiography.	disuccinimidyl	121-135	interferon gamma	Homo sapiens	12-21
2466913-2	1988	When [32P]HuIFN-gamma was bound and crosslinked to IFN-gamma the receptor of human cells with a bifunctional crosslinker disuccinimidyl suberate (DSS), a single diffused 32P-labeled band corresponding to the IFN-gamma.receptor complex was visualized by SDS-polyacrylamide gel electrophoresis and autoradiography.	disuccinimidyl	121-135	interferon gamma	Homo sapiens	51-60
2894373-6	1988	Two ANP-binding sites of 66,000 and 130,000 daltons were specifically labeled by 125I-ANP after cross-linking with disuccinimidyl suberate.	disuccinimidyl	115-129	natriuretic peptide A	Bos taurus	4-7
2894373-6	1988	Two ANP-binding sites of 66,000 and 130,000 daltons were specifically labeled by 125I-ANP after cross-linking with disuccinimidyl suberate.	disuccinimidyl	115-129	natriuretic peptide A	Bos taurus	86-89
3355513-1	1988	Lactogenic receptors were analysed with the use of the cross-linking agent disuccinimidyl suberate to attach covalently 125I-labelled ovine prolactin or human growth hormone to binding sites from (1) liver from pregnant rats and (2) the rat-derived Nb2 lymphoma cell line.	disuccinimidyl	75-89	prolactin	Homo sapiens	140-149
3355513-1	1988	Lactogenic receptors were analysed with the use of the cross-linking agent disuccinimidyl suberate to attach covalently 125I-labelled ovine prolactin or human growth hormone to binding sites from (1) liver from pregnant rats and (2) the rat-derived Nb2 lymphoma cell line.	disuccinimidyl	75-89	growth hormone 1	Homo sapiens	159-173
3277630-3	1988	[125I]insulin-receptor complex was incubated with various amounts of IDE at 0 degree C in the presence of disuccinimidyl suberate and analyzed by reduced 7.5% SDS-PAGE and autoradiography.	disuccinimidyl	106-120	insulin	Homo sapiens	6-13
3335512-3	1988	The cells were incubated with 125I-human chorionic gonadotropin (hCG) following which the bound 125I-hCG was covalently cross-linked to the cell surface receptor using a cleavable (dithiobis(succinimidyl propionate] and a noncleavable (disuccinimidyl suberate) cross-linking reagent.	disuccinimidyl	236-250	chorionic gonadotropin subunit beta 5	Homo sapiens	101-104
3164434-6	1988	[125I]TGF beta was cross-linked to Leydig cells using disuccinimidyl suberate; cells affinity labelled with [125I]TGF beta exhibit a major labelled band of approx 280 kDa, which has the properties expected from a TGF beta receptor.	disuccinimidyl	54-68	transforming growth factor beta 1	Homo sapiens	6-14
3308583-7	1987	Covalent cross-linking of labeled insulin to BLM insulin receptors with disuccinimidyl suberate revealed a single 135,000-Mr band that was completely inhibited by unlabeled insulin.	disuccinimidyl	72-86	insulin	Canis lupus familiaris	34-41
2822405-5	1987	Covalent binding of 125I-erythropoietin to its receptors with a cross-linking reagent, disuccinimidyl suberate or glutaraldehyde, resulted in the formation of two major radiolabeled products that migrated as 120-kDa and 140-kDa species on sodium dodecyl sulfate/polyacrylamide electrophoresis gels under reducing conditions.	disuccinimidyl	87-101	erythropoietin	Mus musculus	25-39
3498628-3	1987	Affinity labeling with [125I]iodo-EGF and subsequent cross-linking with disuccinimidyl suberate demonstrated the specific binding of [125I]iodo-EGF to a macromolecule with a mol wt of 170,000.	disuccinimidyl	72-86	epidermal growth factor	Rattus norvegicus	144-147
2823637-3	1987	[125I]SP was specifically crosslinked by disuccinimidyl suberate to IM-9 cell membrane proteins of 78, 58, and 33 kDa.	disuccinimidyl	41-55	tachykinin precursor 1	Homo sapiens	6-8
2968745-5	1988	Cross-linking of 125I-IGF-I to the receptor with disuccinimidyl suberate, followed by SDS-polyacrylamide gel electrophoresis under reducing conditions showed an IGF-I binding protein with a molecular weight of 146,000 Dalton.	disuccinimidyl	49-63	IGFI	Bos taurus	161-166
3035548-5	1987	125I-Epo binding reached equilibrium within 20 min at 37 degrees C. Twenty percent of the receptors bound 125I-Epo with a Kd of 0.08 X 10(-9) M, while the remaining receptors bound the hormone with a Kd of 0.6 X 10(-9) M. In this study, a receptor for Epo was identified by cross-linking 125I-Epo to the receptor in intact cells and plasma membrane preparations using disuccinimidyl suberate.	disuccinimidyl	368-382	erythropoietin	Mus musculus	5-8
3035570-5	1987	Membrane components that specifically interact with 125I-Epo were identified by covalent crosslinking with disuccinimidyl suberate, and three receptor species with apparent Mr 63,000, 94,000, and 119,000 were found in membrane from SKT6 cells, suggesting the complex structure of the receptor molecules.	disuccinimidyl	107-121	erythropoietin	Mus musculus	57-60
2889449-5	1987	The molecular characteristics of ANF binding sites were examined by affinity cross-linking of 125I-ANF to intact cells with disuccinimidyl suberate.	disuccinimidyl	124-138	natriuretic peptide A	Rattus norvegicus	33-36
2889449-5	1987	The molecular characteristics of ANF binding sites were examined by affinity cross-linking of 125I-ANF to intact cells with disuccinimidyl suberate.	disuccinimidyl	124-138	natriuretic peptide A	Rattus norvegicus	99-102
2952646-1	1987	When mouse L cells are incubated with 32P-labeled recombinant murine immune interferon ( [32P]Mu-IFN-gamma) and subsequently cross-linked with disuccinimidyl suberate, a major complex with an apparent molecular mass of 95,000-125,000 daltons can be visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.	disuccinimidyl	143-157	interferon gamma	Mus musculus	97-106
3614558-1	1987	To investigate the physicochemical characteristics of angiotensin II receptors in nervous tissue, [125I]-labeled AII was covalently linked to its binding sites in cultured mouse spinal cord cells, using the chemical crosslinking agent disuccinimidyl suberate.	disuccinimidyl	235-249	arginase type II	Mus musculus	113-116
3031028-4	1987	Treatment of surface-bound 125I-hemopexin with divalent lysine-directed cross-linking disuccinimidyl suberate revealed a membrane polypeptide of about 80,000 Da, to which hemopexin is cross-linked.	disuccinimidyl	86-100	hemopexin	Homo sapiens	32-41
3031028-4	1987	Treatment of surface-bound 125I-hemopexin with divalent lysine-directed cross-linking disuccinimidyl suberate revealed a membrane polypeptide of about 80,000 Da, to which hemopexin is cross-linked.	disuccinimidyl	86-100	hemopexin	Homo sapiens	171-180
2435170-2	1987	To characterize the molecular components of these receptors, 125I-VIP was covalently cross-linked to these receptors by four different cross-linking agents: disuccinimidyl suberate, ethylene glycol bis (succinimidyl succinate), dithiobis (succinimidylpropionate), and m-maleimidobenzoyl N-hydroxysuccinimide ester.	disuccinimidyl	157-171	vasoactive intestinal peptide	Rattus norvegicus	66-69
3026782-1	1987	A cytosolic insulin-degrading enzyme (Mr = 110,000) was found to be cross-linked to [125I]-insulin in intact human hepatoma cells, HepG2, incubated with the hormone and treated with the bifunctional cross-linker, disuccinimidyl suberate.	disuccinimidyl	213-227	insulin degrading enzyme	Homo sapiens	12-36
3026782-1	1987	A cytosolic insulin-degrading enzyme (Mr = 110,000) was found to be cross-linked to [125I]-insulin in intact human hepatoma cells, HepG2, incubated with the hormone and treated with the bifunctional cross-linker, disuccinimidyl suberate.	disuccinimidyl	213-227	insulin	Homo sapiens	12-19
3812684-3	1987	125I-gastrin2(-17) was cross-linked to plasma membranes or intact parietal cells by incubation in disuccinimidyl suberate.	disuccinimidyl	98-112	gastrin	Canis lupus familiaris	5-12
3023363-3	1986	We have attempted to label the GRF receptor by chemically coupling the 125I-GRF analog [His1, Nle27]-hGRF(1-32)-NH2 (GRFa) (where Nle is norleucine) to plated rat anterior pituitary cells with the protein cross-linker disuccinimidyl suberate (DSS) (0.1 mM).	disuccinimidyl	218-232	growth hormone releasing hormone receptor	Rattus norvegicus	31-43
3023363-3	1986	We have attempted to label the GRF receptor by chemically coupling the 125I-GRF analog [His1, Nle27]-hGRF(1-32)-NH2 (GRFa) (where Nle is norleucine) to plated rat anterior pituitary cells with the protein cross-linker disuccinimidyl suberate (DSS) (0.1 mM).	disuccinimidyl	218-232	growth hormone releasing hormone	Rattus norvegicus	31-34
2958982-5	1987	Results from disuccinimidyl suberate cross-linking of 125I-IGF-I to muscle-derived ovine fibroblasts in the presence of related polypeptides verified the competitive binding data.	disuccinimidyl	13-27	insulin like growth factor 1	Homo sapiens	59-64
2948816-9	1987	Disuccinimidyl suberate cross-linked [125I]IGF-I to a moiety in FRTL-5 that had an apparent mol wt of approximately 135,000, as judged from sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions.	disuccinimidyl	0-14	insulin-like growth factor 1	Rattus norvegicus	43-48
2429956-7	1986	Affinity cross-linking of 125I-SP by disuccinimidyl suberate to intact IM-9 cells and membranes revealed specifically labeled proteins of Mr 58,000 and 33,000 in cells, and 58,000, 33,000, and 16,000 in membranes by sodium dodecyl sulfate-polyacrylamide gel electrophoresis under both reducing and nonreducing conditions.	disuccinimidyl	37-51	tachykinin precursor 1	Homo sapiens	31-33
3793398-4	1986	The 125I-insulin-protein complexes were covalently cross-linked with disuccinimidyl suberate and chromatographed on a Sepharose CL-6B column.	disuccinimidyl	69-83	insulin	Bos taurus	9-16
3017969-1	1986	A gastrin binding protein (GBP) has been identified in detergent extracts of porcine gastric mucosal membranes by covalent cross-linking to 125I-[Nle15]gastrin with disuccinimidyl suberate.	disuccinimidyl	165-179	gastrin	Canis lupus familiaris	2-9
2944578-6	1986	Cross-linking of membrane-bound 125I-IFN-gamma with disuccinimidyl suberate and subsequent sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed, in both leukemia and carcinoma cells, three distinct complexes with molecular weights of approximately 70,000, 92,000, and 160,000, suggesting the existence of IFN-gamma receptor subunits.	disuccinimidyl	52-66	interferon gamma	Homo sapiens	37-46
3017969-1	1986	A gastrin binding protein (GBP) has been identified in detergent extracts of porcine gastric mucosal membranes by covalent cross-linking to 125I-[Nle15]gastrin with disuccinimidyl suberate.	disuccinimidyl	165-179	gastrin	Canis lupus familiaris	152-159
3743768-1	1986	Binding of 125I-gastrin to porcine gastric transferrin has been demonstrated by covalent cross-linking with disuccinimidyl suberate.	disuccinimidyl	108-122	gastrin	Homo sapiens	16-23
3019243-4	1986	The mean number of binding sites per cell was calculated to be 12,000, and the apparent dissociation constant (Kd) was 2 X 10(-10) M. Crosslinking of 125I-TNF to the cell surface receptor with disuccinimidyl suberate, followed by NaDodSO4-polyacrylamide gel electrophoresis of the cell lysate, revealed a TNF-receptor complex with a molecular weight of approximately 100,000.	disuccinimidyl	193-207	tumor necrosis factor	Homo sapiens	155-158
3019243-4	1986	The mean number of binding sites per cell was calculated to be 12,000, and the apparent dissociation constant (Kd) was 2 X 10(-10) M. Crosslinking of 125I-TNF to the cell surface receptor with disuccinimidyl suberate, followed by NaDodSO4-polyacrylamide gel electrophoresis of the cell lysate, revealed a TNF-receptor complex with a molecular weight of approximately 100,000.	disuccinimidyl	193-207	tumor necrosis factor	Homo sapiens	305-308
3743768-1	1986	Binding of 125I-gastrin to porcine gastric transferrin has been demonstrated by covalent cross-linking with disuccinimidyl suberate.	disuccinimidyl	108-122	transferrin	Homo sapiens	43-54
2874135-1	1986	Affinity labeling of target cells for transforming growth factor-beta (TGF beta) by cross-linking with 125I-TGF beta via disuccinimidyl suberate or by the photoreactive analogue 4-azidobenzoyl-125I-TGF beta has revealed the presence of multiple TGF beta receptor forms.	disuccinimidyl	121-135	transforming growth factor beta 1	Homo sapiens	38-69
3023310-13	1986	The turnover of TNF receptors on HeLA S3 cells seemed to be rapid, since the level of specific binding quickly decreased after treatment with 100 micrograms/ml of cycloheximide at 37 degrees C with a half-life of about 1.5 h. The crosslinking of the cell-bound 125I-TNF with the use of disuccinimidyl suberate yielded a complex of 105 kDa for HeLa S3 and THP-1 cells, and a complex of 100 kDa for U937 cells.	disuccinimidyl	286-300	tumor necrosis factor	Homo sapiens	16-19
3025822-4	1986	125I-VIP has been covalently incorporated into lung membranes using disuccinimidyl suberate.	disuccinimidyl	68-82	vasoactive intestinal peptide	Homo sapiens	5-8
2874135-1	1986	Affinity labeling of target cells for transforming growth factor-beta (TGF beta) by cross-linking with 125I-TGF beta via disuccinimidyl suberate or by the photoreactive analogue 4-azidobenzoyl-125I-TGF beta has revealed the presence of multiple TGF beta receptor forms.	disuccinimidyl	121-135	transforming growth factor beta 1	Homo sapiens	71-79
2874135-1	1986	Affinity labeling of target cells for transforming growth factor-beta (TGF beta) by cross-linking with 125I-TGF beta via disuccinimidyl suberate or by the photoreactive analogue 4-azidobenzoyl-125I-TGF beta has revealed the presence of multiple TGF beta receptor forms.	disuccinimidyl	121-135	transforming growth factor beta 1	Homo sapiens	108-116
2874135-1	1986	Affinity labeling of target cells for transforming growth factor-beta (TGF beta) by cross-linking with 125I-TGF beta via disuccinimidyl suberate or by the photoreactive analogue 4-azidobenzoyl-125I-TGF beta has revealed the presence of multiple TGF beta receptor forms.	disuccinimidyl	121-135	transforming growth factor beta 1	Homo sapiens	108-116
2874135-1	1986	Affinity labeling of target cells for transforming growth factor-beta (TGF beta) by cross-linking with 125I-TGF beta via disuccinimidyl suberate or by the photoreactive analogue 4-azidobenzoyl-125I-TGF beta has revealed the presence of multiple TGF beta receptor forms.	disuccinimidyl	121-135	transforming growth factor beta 1	Homo sapiens	108-116
3087891-4	1986	Disuccinimidyl-suberate cross-linking of receptor-bound 125I-TNF-alpha and SDS-PAGE of membrane preparations of either U937 or K562 cells suggest a single receptor protein with an apparent molecular weight of 76 kDa.	disuccinimidyl	0-14	tumor necrosis factor	Homo sapiens	61-70
3527844-2	1986	However, after 125I-insulin was covalently cross-linked to liver membranes using disuccinimidyl suberate in the presence of anti-insulin antibody, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography showed that 125I-insulin bound to the alpha-subunit of the insulin receptor was inhibited by anti-insulin insulin antibody in an dose-dependent manner.	disuccinimidyl	81-95	insulin	Homo sapiens	20-27
3011572-5	1986	The IGF I receptor was further characterized by reduced polyacrylamide gel electrophoresis of the disuccinimidyl suberate cross-linked 125I-labeled IGF I receptor.	disuccinimidyl	98-112	insulin like growth factor 1 receptor	Homo sapiens	4-18
3011572-5	1986	The IGF I receptor was further characterized by reduced polyacrylamide gel electrophoresis of the disuccinimidyl suberate cross-linked 125I-labeled IGF I receptor.	disuccinimidyl	98-112	insulin like growth factor 1 receptor	Homo sapiens	148-162
3718510-3	1986	When 125I-IGF-1 was cross-linked to inhibitor using disuccinimidyl suberate, five specifically labelled bands were seen on SDS-PAGE and autoradiography.	disuccinimidyl	52-66	insulin like growth factor 1	Homo sapiens	10-15
3003157-1	1986	To identify the molecular site of thrombin binding to the platelet membrane, we covalently linked 125I-thrombin to platelets by using the bifunctional chemical cross-linking agents disuccinimidyl suberate and dithiobis(succinimidyl propionate).	disuccinimidyl	181-195	coagulation factor II, thrombin	Homo sapiens	103-111
2426267-3	1986	The IGF-binding protein is efficiently and specifically cross-linked to either 125I-IGF-I (human) or 125I-IGF-II (rat) using disuccinimidyl suberate.	disuccinimidyl	125-139	insulin like growth factor 2	Homo sapiens	106-112
3008661-1	1986	The subunit composition of the thyrotropin (TSH) receptor has been characterized using the bifunctional crosslinking agent, disuccinimidyl suberate (DSS), to covalently link [125I]TSH to its receptor.	disuccinimidyl	124-138	thyroid stimulating hormone receptor	Homo sapiens	4-57
3004907-1	1986	We have used a bifunctional cross-linker, disuccinimidyl suberate, to covalently attach [125I]human pancreatic GH-releasing factor (GHRF) (-1-40)OH to bovine pituitary membranes and rat anterior pituitary cells.	disuccinimidyl	42-56	growth hormone releasing hormone	Homo sapiens	132-136
3001089-7	1986	The insulin receptor made in the presence of the inhibitor can be affinity labeled at the cell surface using 125I-insulin and disuccinimidyl suberate cross-linking; there is also no significant difference in its affinity for insulin.	disuccinimidyl	126-140	insulin receptor	Homo sapiens	4-20
3001089-7	1986	The insulin receptor made in the presence of the inhibitor can be affinity labeled at the cell surface using 125I-insulin and disuccinimidyl suberate cross-linking; there is also no significant difference in its affinity for insulin.	disuccinimidyl	126-140	insulin	Homo sapiens	4-11
2999143-4	1985	Under nonreducing conditions, disuccinimidyl suberate cross-linked 125I-IGF I and 125I-IGF II to two receptor complexes with apparent Mr greater than 300,000 (type I) and 220,000 (type II).	disuccinimidyl	30-44	insulin like growth factor 2	Homo sapiens	72-93
3510133-1	1986	The structure of the insulin receptor in intact human erythrocytes was defined using the techniques of disuccinimidyl suberate (DSS) cross-linking of 125I-insulin and surface [125I]iodination followed by receptor immunoprecipitation.	disuccinimidyl	103-117	insulin receptor	Homo sapiens	21-37
3510133-1	1986	The structure of the insulin receptor in intact human erythrocytes was defined using the techniques of disuccinimidyl suberate (DSS) cross-linking of 125I-insulin and surface [125I]iodination followed by receptor immunoprecipitation.	disuccinimidyl	103-117	insulin	Homo sapiens	21-28
2934742-1	1986	When 32P-labeled human recombinant immune interferon gamma (Hu-[32P]IFN-gamma) is crosslinked to human cells with disuccinimidyl suberate, a complex with a molecular size of approximately equal to 117,000 Da was identified by sodium dodecyl sulfate/polyacrylamide gel electrophoresis.	disuccinimidyl	114-128	interferon gamma	Homo sapiens	68-77
2995005-6	1985	When [125I]insulin and [125I]IGF-I were cross-linked to GH3 cells with disuccinimidyl suberate, followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, both receptors were found to have an apparent mol wt greater than 300,000 in the unreduced state, with subunits of apparent mol wt 125,000 after reduction.	disuccinimidyl	71-85	insulin-like growth factor 1	Rattus norvegicus	29-34
3010256-1	1985	Neurotensin receptors from plasma membranes of rat gastric fundus smooth muscle were specifically and covalently labeled either by using the photoreactive analogue 125I-labeled azidobenzoyl (Trp11)-neurotensin or by cross-linking (monoiodo-Tyr3)neurotensin to the membrane preparation by means of disuccinimidyl suberate.	disuccinimidyl	297-311	neurotensin	Rattus norvegicus	0-11
2993308-10	1985	The IGF-II binding activity of the three different biosynthetic forms of the receptor was assessed by affinity cross-linking of 125I-IGF-II to the receptors using disuccinimidyl suberate.	disuccinimidyl	163-177	insulin-like growth factor 2	Rattus norvegicus	4-10
2994048-13	1985	125I-TNF, covalently cross-linked to its receptor on sensitive L-M cells with disuccinimidyl suberate, was isolated and analyzed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and autoradiography.	disuccinimidyl	78-92	tumor necrosis factor	Homo sapiens	5-8
2988913-3	1985	Incubation of [125I] iodo-EGF with uterine membranes, followed by chemical cross-linking with disuccinimidyl suberate and detergent extraction reveals a major species of specifically bound EGF (mol wt = 170,000) and a minor species (mol wt = 150,000) visualized by autoradiography of sodium dodecyl sulfate gels after electrophoresis of the extracts.	disuccinimidyl	94-108	epidermal growth factor	Rattus norvegicus	189-192
3997862-3	1985	Cross-linking 125I-C5a to intact PMN with disuccinimidyl suberate produced a species that had a molecular mass on sodium dodecyl sulfate gels of 5.2 X 10(4) daltons.	disuccinimidyl	42-56	complement C5a receptor 1	Homo sapiens	19-22
3888982-1	1985	Cytosol extracts high in insulin-degrading activity were cross-linked to 125I-insulin with the bifunctional cross-linker disuccinimidyl suberate.	disuccinimidyl	121-135	insulin	Homo sapiens	25-32
3888982-1	1985	Cytosol extracts high in insulin-degrading activity were cross-linked to 125I-insulin with the bifunctional cross-linker disuccinimidyl suberate.	disuccinimidyl	121-135	insulin	Homo sapiens	78-85
2982593-2	1985	Cross-linking of solubilized [125I]iodohuman GH-receptor complexes with disuccinimidyl suberate followed by analysis by sodium dodecyl sulfate-gel electrophoresis in the presence of beta-mercaptoethanol and autoradiography resulted in the appearance of bands with apparent mol wt of 113,000, 103,000, 59,000, and 53,000.	disuccinimidyl	72-86	growth hormone receptor	Rattus norvegicus	45-56
2981215-4	1985	In the second, the reversible association between neurotensin receptors and 125I-labeled[Trp11]neurotensin, a radioactive but nonphotoreactive analogue of neurotensin, was made irreversible by means of disuccinimidyl suberate, a bifunctional cross-linking reagent.	disuccinimidyl	202-216	neurotensin	Rattus norvegicus	50-61
6088554-0	1984	Analysis of cholecystokinin-binding proteins using endo-beta-N-acetylglucosaminidase F. We have previously shown that the cholecystokinin (CCK)-binding proteins in rat pancreatic plasma membranes consist of a major Mr 85,000 and minor Mr 55,000 and Mr 130,000 species as revealed by affinity labeling with 125I-CCK-33 using the cross-linker, disuccinimidyl suberate.	disuccinimidyl	342-356	cholecystokinin	Rattus norvegicus	139-142
6378598-4	1984	Binding of [125I]insulin to retinal microvessels, followed by covalent cross-linking of the bound ligand to the alpha-subunit of the insulin receptor with the bifunctional reagent disuccinimidyl suberate, yielded a prominent specific [125I]insulin-labeled band when analyzed by sodium dodecyl sulfate-gel electrophoresis followed by autoradiography, and this band had a mobility identical to that of the corresponding complex obtained with rat liver plasma membranes (mol wt, 125,000).	disuccinimidyl	180-194	insulin	Bos taurus	133-140
6378598-4	1984	Binding of [125I]insulin to retinal microvessels, followed by covalent cross-linking of the bound ligand to the alpha-subunit of the insulin receptor with the bifunctional reagent disuccinimidyl suberate, yielded a prominent specific [125I]insulin-labeled band when analyzed by sodium dodecyl sulfate-gel electrophoresis followed by autoradiography, and this band had a mobility identical to that of the corresponding complex obtained with rat liver plasma membranes (mol wt, 125,000).	disuccinimidyl	180-194	insulin	Bos taurus	133-140
6378700-2	1984	After covalent cross-linking of 125I-insulin to the insulin receptor on cultured human lymphocytes (IM-9 cells) using disuccinimidyl suberate, we inquired whether the insulin-receptor complex could be immunoprecipitated with anti-insulin antibodies.	disuccinimidyl	118-132	insulin receptor	Homo sapiens	52-68
6378700-2	1984	After covalent cross-linking of 125I-insulin to the insulin receptor on cultured human lymphocytes (IM-9 cells) using disuccinimidyl suberate, we inquired whether the insulin-receptor complex could be immunoprecipitated with anti-insulin antibodies.	disuccinimidyl	118-132	insulin	Homo sapiens	52-59
6378702-4	1984	Maximum binding at 37 degrees C occurred at 90 min, and was 3.8%/mg protein and, at 15 degrees C, 7%/mg protein at 4 h. 125I-insulin was crosslinked to its receptor using disuccinimidyl suberate (DSS), and the structure of the receptor complex was identified by SDS-polyacrylamide gel electrophoresis and autoradiography; a major band with Mr = 145,000 was identified, which corresponds to the alpha-subunit of the insulin receptor reported in other tissues.	disuccinimidyl	171-185	insulin	Bos taurus	125-132
2986151-9	1985	Affinity labeling of the MSA receptor using the chemical crosslinking reagent, disuccinimidyl suberate, confirmed that this receptor was of the type II class of somatomedin receptors and exhibited a molecular weight of 218,000 under nonreducing conditions.	disuccinimidyl	79-93	insulin-like growth factor 1	Rattus norvegicus	161-172
2580858-2	1985	By using disuccinimidyl suberate, we have covalently cross-linked 125I-labeled somatomedin-C (Sm-C)/insulinlike growth factor I to specific binding proteins in human plasma.	disuccinimidyl	9-23	insulin like growth factor 1	Homo sapiens	79-92
2983665-10	1985	Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis separation of the 125I-EGF-receptor complex from RIE-1 cells after covalently cross-linking with disuccinimidyl suberate indicated a receptor of Mr congruent to 160 000.	disuccinimidyl	156-170	epidermal growth factor like 1	Rattus norvegicus	82-85
2981215-4	1985	In the second, the reversible association between neurotensin receptors and 125I-labeled[Trp11]neurotensin, a radioactive but nonphotoreactive analogue of neurotensin, was made irreversible by means of disuccinimidyl suberate, a bifunctional cross-linking reagent.	disuccinimidyl	202-216	neurotensin	Rattus norvegicus	95-106
2981215-4	1985	In the second, the reversible association between neurotensin receptors and 125I-labeled[Trp11]neurotensin, a radioactive but nonphotoreactive analogue of neurotensin, was made irreversible by means of disuccinimidyl suberate, a bifunctional cross-linking reagent.	disuccinimidyl	202-216	neurotensin	Rattus norvegicus	95-106
6330113-3	1984	Affinity labeling of the 170,000-dalton EGF receptor with 125I-EGF and disuccinimidyl suberate was also decreased by the tumor promoter at low, but not high, concentrations of 125I-EGF.	disuccinimidyl	71-85	epidermal growth factor receptor	Homo sapiens	40-52
6330113-3	1984	Affinity labeling of the 170,000-dalton EGF receptor with 125I-EGF and disuccinimidyl suberate was also decreased by the tumor promoter at low, but not high, concentrations of 125I-EGF.	disuccinimidyl	71-85	epidermal growth factor	Homo sapiens	40-43
6323477-2	1984	125I-Human growth hormone was cross-linked with high efficiency to lactogen receptors in membranes and in Triton X-100-solubilized preparations from luteinized rat ovaries using the bifunctional reagent disuccinimidyl suberate.	disuccinimidyl	203-217	growth hormone 1	Homo sapiens	11-25
6321495-3	1984	A native insulin receptor species with apparent Mr = 350,000 in soleus muscle is revealed by affinity cross-linking to 125I-insulin with disuccinimidyl suberate.	disuccinimidyl	137-151	insulin receptor	Rattus norvegicus	9-25
6321506-7	1984	The proteins eluted from the region of the gel when cross-linked to 125I-IGF-II with disuccinimidyl suberate and electrophoresed in the presence of sodium dodecyl sulfate have the same molecular weight, Mr, as the previously described IGF-II receptor.	disuccinimidyl	85-99	insulin-like growth factor 2	Rattus norvegicus	73-79
6322783-2	1984	The receptor, labeled by crosslinking to 125I-CSF-1 with disuccinimidyl suberate, is a polypeptide of approximately 165,000 molecular weight, irrespective of cell type.	disuccinimidyl	57-71	colony stimulating factor 1 (macrophage)	Mus musculus	46-51
6420403-3	1984	2) Cross-linking 125I-calmodulin to whole MAPs and MAP2 by disuccinimidyl suberate: 125I-calmodulin was cross-linked to MAP2 and tau proteins showing an intense radioactivity band at 300,000 daltons and a diffuse band between 70,000 and 90,000 daltons.	disuccinimidyl	59-73	calmodulin 1	Homo sapiens	22-32
6420403-3	1984	2) Cross-linking 125I-calmodulin to whole MAPs and MAP2 by disuccinimidyl suberate: 125I-calmodulin was cross-linked to MAP2 and tau proteins showing an intense radioactivity band at 300,000 daltons and a diffuse band between 70,000 and 90,000 daltons.	disuccinimidyl	59-73	microtubule associated protein 2	Homo sapiens	51-55
6420403-3	1984	2) Cross-linking 125I-calmodulin to whole MAPs and MAP2 by disuccinimidyl suberate: 125I-calmodulin was cross-linked to MAP2 and tau proteins showing an intense radioactivity band at 300,000 daltons and a diffuse band between 70,000 and 90,000 daltons.	disuccinimidyl	59-73	calmodulin 1	Homo sapiens	89-99
6361025-9	1983	Insulin receptors were covalently attached to [125I]iodoinsulin with disuccinimidyl suberate.	disuccinimidyl	69-83	insulin	Meleagris gallopavo	0-7
6355185-6	1983	The insulin-binding sites of liver membranes were affinity-labeled with 125I-insulin and the cross-linking reagent, disuccinimidyl suberate.	disuccinimidyl	116-130	insulin	Homo sapiens	4-11
6331415-6	1984	Disuccinimidyl suberate covalently coupled 125I-human somatotropin into saturable complexes of Mr 300 000, 220 000, 130 000, 65 000 and 50 000.	disuccinimidyl	0-14	growth hormone 1	Homo sapiens	54-66
6311206-1	1983	125I-labeled human somatotropin specifically bound to the lactogenic sites of microsomal membranes from pregnant rat liver, originated a radioactive covalent complex of Mr 63,000 upon reaction with dimethyl suberimidate, disuccinimidyl suberate (DSS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide.	disuccinimidyl	221-235	growth hormone 1	Homo sapiens	19-31
6311548-9	1983	Covalent binding of the radioiodinated angiotensin II to a single, specific macromolecular component was achieved by treatment with disuccinimidyl suberate.	disuccinimidyl	132-146	angiotensinogen	Homo sapiens	39-53
6873516-1	1983	125I-insulin was covalently crosslinked to its receptor on human placental membrane fractions with disuccinimidyl suberate.	disuccinimidyl	99-113	insulin	Homo sapiens	5-12
6348041-6	1983	After brief or extended incubations with hormone, during which the fraction of higher affinity receptors increased, 125I-insulin was covalently coupled to the alpha subunits of its receptor using disuccinimidyl suberate.	disuccinimidyl	196-210	insulin	Homo sapiens	121-128
6313160-1	1983	Human placental receptors for insulin and for human basic somatomedin (BSM, analogous to human insulinlike growth factor I (IGF-I] were selectively cross-link labelled with 125I-labelled insulin and BSM using disuccinimidyl suberate under conditions whereby cross-linking of 125I-labelled insulin to the BSM receptor and of 125I-labelled BSM to the insulin receptor was avoided.	disuccinimidyl	209-223	insulin like growth factor 1	Homo sapiens	95-122
6313160-1	1983	Human placental receptors for insulin and for human basic somatomedin (BSM, analogous to human insulinlike growth factor I (IGF-I] were selectively cross-link labelled with 125I-labelled insulin and BSM using disuccinimidyl suberate under conditions whereby cross-linking of 125I-labelled insulin to the BSM receptor and of 125I-labelled BSM to the insulin receptor was avoided.	disuccinimidyl	209-223	insulin like growth factor 1	Homo sapiens	124-129
6313160-1	1983	Human placental receptors for insulin and for human basic somatomedin (BSM, analogous to human insulinlike growth factor I (IGF-I] were selectively cross-link labelled with 125I-labelled insulin and BSM using disuccinimidyl suberate under conditions whereby cross-linking of 125I-labelled insulin to the BSM receptor and of 125I-labelled BSM to the insulin receptor was avoided.	disuccinimidyl	209-223	insulin	Homo sapiens	95-102
6313160-1	1983	Human placental receptors for insulin and for human basic somatomedin (BSM, analogous to human insulinlike growth factor I (IGF-I] were selectively cross-link labelled with 125I-labelled insulin and BSM using disuccinimidyl suberate under conditions whereby cross-linking of 125I-labelled insulin to the BSM receptor and of 125I-labelled BSM to the insulin receptor was avoided.	disuccinimidyl	209-223	insulin	Homo sapiens	95-102
6841449-2	1983	To define the molecular properties of the CCK-binding site, we incubated rat pancreatic plasma membranes with 125-I-CCK-33 for 15 min at 23 degrees C followed by washing and cross-linking with disuccinimidyl suberate.	disuccinimidyl	193-207	cholecystokinin	Rattus norvegicus	42-45
6300099-7	1983	These results were confirmed by direct visualization of the Mr = 270,000 IGF-II receptor band on dodecyl sulfate gels following affinity labeling with 125I-IGF-II and the cross-linker disuccinimidyl suberate.	disuccinimidyl	184-198	insulin-like growth factor 2	Rattus norvegicus	73-79
6833290-3	1983	In contrast, when the 125I-insulin-treated membrane sample was extracted with Triton X-102, purified by DEAE-Sephacel ion exchange chromatography, covalently cross-linked with disuccinimidyl suberate, and then subjected to SDS-polyacrylamide gel electrophoresis, a highly radioactive component with Mr = 53,000 was obtained.	disuccinimidyl	176-190	insulin	Homo sapiens	27-34
6297990-3	1983	When [125I]CCK was cross-linked to its receptors with disuccinimidyl suberate, and the preparation solubilized and subjected to gel electrophoresis and autoradiography, the hormone was associated with Mr 80 000 protein in both the presence and absence of the reducing agent dithiothreitol.	disuccinimidyl	54-68	cholecystokinin	Mus musculus	11-14
6889490-8	1982	When photolabeled ABP was treated with the cross-linking reagent disuccinimidyl suberate before electrophoresis on gels containing sodium dodecyl sulfate, an additional androgen-specific peak corresponding to approximately 100,000 daltons was obtained.	disuccinimidyl	65-79	sex hormone-binding globulin	Oryctolagus cuniculus	18-21
6285362-1	1982	To identify the molecular components of the angiotensin II (AII) receptor in the adrenal cortex, we have covalently linked 125I-labeled AII (125I-AII) to its binding sites by using the chemical crosslinker disuccinimidyl suberate.	disuccinimidyl	206-220	angiotensinogen	Rattus norvegicus	44-58
6285362-1	1982	To identify the molecular components of the angiotensin II (AII) receptor in the adrenal cortex, we have covalently linked 125I-labeled AII (125I-AII) to its binding sites by using the chemical crosslinker disuccinimidyl suberate.	disuccinimidyl	206-220	angiotensinogen	Rattus norvegicus	60-63
6285362-1	1982	To identify the molecular components of the angiotensin II (AII) receptor in the adrenal cortex, we have covalently linked 125I-labeled AII (125I-AII) to its binding sites by using the chemical crosslinker disuccinimidyl suberate.	disuccinimidyl	206-220	angiotensinogen	Rattus norvegicus	136-139
6285362-1	1982	To identify the molecular components of the angiotensin II (AII) receptor in the adrenal cortex, we have covalently linked 125I-labeled AII (125I-AII) to its binding sites by using the chemical crosslinker disuccinimidyl suberate.	disuccinimidyl	206-220	angiotensinogen	Rattus norvegicus	136-139
6275879-1	1981	Human placental receptors for insulin and somatomedin C (Sm-C) were affinity labeled with [125I]insulin and [125I]Sm-C by using the bifunctional cross-linking agent disuccinimidyl suberate.	disuccinimidyl	165-179	insulin like growth factor 1	Homo sapiens	42-55
6756616-2	1982	Further, we have developed conditions under which the basic SM receptor, in the presence of equivalent amounts of insulin receptor, can be selectively and specifically affinity-labeled with 125I-labeled basic SM, using the cross-linking reagent disuccinimidyl suberate (DSS).	disuccinimidyl	245-259	insulin receptor	Homo sapiens	114-130
6756618-5	1982	Pretreatment of cells with unlabelled insulin partially blocked the effect of disuccinimidyl suberate, and as has been previously shown, disuccinimidyl suberate cross-linked insulin to its receptor.	disuccinimidyl	78-92	insulin	Homo sapiens	38-45
6756618-5	1982	Pretreatment of cells with unlabelled insulin partially blocked the effect of disuccinimidyl suberate, and as has been previously shown, disuccinimidyl suberate cross-linked insulin to its receptor.	disuccinimidyl	137-151	insulin	Homo sapiens	38-45
7202866-1	1981	125I-insulin was specifically cross-linked to membranes of human cultured lymphocytes (IM-9 line) using disuccinimidyl suberate.	disuccinimidyl	104-118	insulin	Homo sapiens	5-12
6264462-1	1981	The bifunctional crosslinking reagents disuccinimidyl suberate and dithiobis(succinimidyl propionate) were used to attach 125I-labeled human chorionic gonadotropin (125I-hCG) covalently to rat testicular membranes.	disuccinimidyl	39-53	chorionic gonadotropin subunit beta 5	Homo sapiens	170-173
6986378-2	1980	125I-Insulin equilibrated with high affinity fat cell and liver plasma membrane receptors was cross-linked to the membrane by addition of disuccinimidyl suberate.	disuccinimidyl	138-152	insulin	Homo sapiens	5-12
6986378-11	1980	Since the 125I-insulin used in these studies is labeled predominantly on the A chain, this large loss of label upon reduction indicates that it is predominantly the B chain of insulin that is covalently linked to the receptor protein by disuccinimidyl suberate.	disuccinimidyl	237-251	insulin	Homo sapiens	176-183
6986378-14	1980	It is concluded that the B1 terminal amino and B29 lysine amino groups are both accessible for cross-linking of insulin to the receptor by disuccinimidyl suberate.	disuccinimidyl	139-153	insulin	Homo sapiens	112-119
25164810-5	2014	Using a chemical cross-linker, disuccinimidyl suberate (DSS), we demonstrated that DGAT2 formed a dimer and was also part of a protein complex of ~ 650 kDa, both in membranes and on lipid droplets.	disuccinimidyl	31-45	diacylglycerol O-acyltransferase 2	Rattus norvegicus	83-88