PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 34855283-7 2021 More interestingly, almost all LacdiNAc-containing N-glycopeptides were enhanced in ICC tumor but not in HCC tumor, and this phenomenon was further confirmed by lectin histochemistry and the high expression of beta1-4 GalNAc transferases in ICC at both mRNA and protein expression levels. n-glycopeptides 51-66 immunoglobulin kappa variable 6D-41 (non-functional) Homo sapiens 210-217 17918875-2 2007 The use of alpha(2-3,6,8,9) neuraminidase is central to isolate sialylated N-glycopeptides out of a complex peptide mixture. n-glycopeptides 75-90 neuraminidase 1 Homo sapiens 28-41 17154134-3 2006 It was demonstrated that the ZIC-HILIC separation column has a selectivity for sialylated N-glycopeptides and a high capability for separation based on the structural recognition of sialylated N-glycan isomers as well as for the previously reported neutral N-glycans and N-glycopeptides. n-glycopeptides 90-105 Zic family member 1 Homo sapiens 29-32 17154134-3 2006 It was demonstrated that the ZIC-HILIC separation column has a selectivity for sialylated N-glycopeptides and a high capability for separation based on the structural recognition of sialylated N-glycan isomers as well as for the previously reported neutral N-glycans and N-glycopeptides. n-glycopeptides 271-286 Zic family member 1 Homo sapiens 29-32 34296732-7 2021 The inherent properties endowed the nanocomposite with excellent enrichment performance for N-glycopeptides: excellent selectivity (1 : 2000, IgG/BSA, m/m), an ultralow detection limit (0.05 fmol muL-1), and a good size-exclusion effect (1 : 500, IgG digests/BSA, m/m). n-glycopeptides 92-107 mitochondrial E3 ubiquitin protein ligase 1 Homo sapiens 196-201 34779613-6 2021 Finally, our results illustrated the aberrant sialylation of haptoglobin (Hp) in hepatocellular carcinoma (HCC), where the ratios of alpha2,3 to alpha2,6 sialylation of seven N-glycopeptides were found to be significantly altered (p < 0.01) in HCC individuals (n = 27) compared with healthy controls (n = 27). n-glycopeptides 175-190 haptoglobin Homo sapiens 61-72 34779613-6 2021 Finally, our results illustrated the aberrant sialylation of haptoglobin (Hp) in hepatocellular carcinoma (HCC), where the ratios of alpha2,3 to alpha2,6 sialylation of seven N-glycopeptides were found to be significantly altered (p < 0.01) in HCC individuals (n = 27) compared with healthy controls (n = 27). n-glycopeptides 175-190 glycoprotein hormone subunit alpha 2 Homo sapiens 133-151 34436504-0 2021 PRM-MS Quantitative Analysis of Isomeric N-Glycopeptides Derived from Human Serum Haptoglobin of Patients with Cirrhosis and Hepatocellular Carcinoma. n-glycopeptides 41-56 haptoglobin Homo sapiens 82-93 34436504-6 2021 Strategic combinations of the significant N-glycopeptides, either with alpha-fetoprotein (AFP) or themselves, better estimate the areas under the curve (AUC) of their respective receiver operating characteristic (ROC) curves with respect to AFP. n-glycopeptides 42-57 alpha fetoprotein Homo sapiens 90-93 34436504-6 2021 Strategic combinations of the significant N-glycopeptides, either with alpha-fetoprotein (AFP) or themselves, better estimate the areas under the curve (AUC) of their respective receiver operating characteristic (ROC) curves with respect to AFP. n-glycopeptides 42-57 alpha fetoprotein Homo sapiens 241-244 34436504-7 2021 The combination of AFP with the isomeric sialylated fucosylated N-glycopeptides Asn207 + 5-6-1-2 and Asn207 + 5-6-1-3, resulted with an AUC value of 0.98, while the AUC value for AFP alone was 0.85. n-glycopeptides 64-79 alpha fetoprotein Homo sapiens 19-22 34436504-7 2021 The combination of AFP with the isomeric sialylated fucosylated N-glycopeptides Asn207 + 5-6-1-2 and Asn207 + 5-6-1-3, resulted with an AUC value of 0.98, while the AUC value for AFP alone was 0.85. n-glycopeptides 64-79 alpha fetoprotein Homo sapiens 179-182 34126284-7 2021 In contrast, the human alpha-l-fucosidase (FucA1) showed low activity on truncated Fucalpha1,6GlcNAc substrates but was able to remove core fucose from intact and full-length core-fucosylated N-glycopeptides and N-glycoproteins. n-glycopeptides 192-207 alpha-L-fucosidase 1 Homo sapiens 43-48 34236361-2 2021 We describe here a facile chemoenzymatic synthesis of core-fucosylated N-glycopeptides derived from the SARS-CoV-2 Spike protein and their binding with glycan-dependent neutralizing antibody S309 and human lectin CLEC4G. n-glycopeptides 71-86 surface glycoprotein Severe acute respiratory syndrome coronavirus 2 115-120 34236361-2 2021 We describe here a facile chemoenzymatic synthesis of core-fucosylated N-glycopeptides derived from the SARS-CoV-2 Spike protein and their binding with glycan-dependent neutralizing antibody S309 and human lectin CLEC4G. n-glycopeptides 71-86 C-type lectin domain family 4 member G Homo sapiens 213-219 35573732-7 2022 Moreover, the alterations of LAMP2 site-specific intact N-glycopeptides were comprehensively assessed. n-glycopeptides 56-71 lysosomal associated membrane protein 2 Homo sapiens 29-34 35510762-6 2022 The experimental results demonstrated that 217 N-glycosylation sites were identified in 283 N-glycopeptides, corresponding to 95 glycoproteins identified from 10 muL human serum by the nano-LC-MS/MS analysis, revealing the great potential of the novel ZIF-8/SAP hydrogel for glycopeptide enrichment and glycoproteomic research. n-glycopeptides 92-107 SH2 domain containing 1A Homo sapiens 258-261 34105527-3 2021 Under the synergistic effect of hydrogen bonding and electrostatic adsorption, Fe3O4@PANI can rapidly and easily enrich N-glycopeptides derived from standard protein (bovine fetuin and transferrin) tryptic digests and serum haptoglobin tryptic digests. n-glycopeptides 120-135 serotransferrin Bos taurus 185-196 34178943-7 2021 We have newly identified systemic site-specific N-glycosylation alterations underlying T2D patients in the complement activation pathways, including decreased levels of N-glycopeptides from C1s, MASP1, and CFP proteins, and increased levels of N-glycopeptides from C2, C4, C4BPA, C4BPB, and CFH. n-glycopeptides 169-184 complement C1s Homo sapiens 190-193 35559953-7 2022 We identified 79 N-glycopeptides from 15 different proteins and found that proteins including immunoglobulin A1, polymeric immunoglobulin receptor, and lactotransferrin displayed significant glycan heterogeneity. n-glycopeptides 17-32 megakaryocyte and platelet inhibitory receptor G6b Homo sapiens 123-146 35559953-7 2022 We identified 79 N-glycopeptides from 15 different proteins and found that proteins including immunoglobulin A1, polymeric immunoglobulin receptor, and lactotransferrin displayed significant glycan heterogeneity. n-glycopeptides 17-32 lactotransferrin Homo sapiens 152-168 35604151-0 2022 A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues. n-glycopeptides 60-75 spindlin 1 Homo sapiens 2-6 35602943-3 2022 Further study showed that indole-3-acetic acid (IAA) levels were undetectable in mns1 mns2 mns3 at normal condition and recovered at alkaline condition, which corroborate our N-glycopeptide profiling, from which N-glycopeptides related with IAA biosynthesis, amino acid conjugates hydrolysis, and response showed differential abundance between normal and alkaline conditions in mns1 mns2 mns3. n-glycopeptides 212-227 alpha-mannosidase 1 Arabidopsis thaliana 81-90 33889548-6 2021 Then, the alteration of site-specific intact N-glycopeptides of PON1 was comprehensively assessed by using Immunoprecipitation (IP) and mass spectrometry based 16O/18O C-terminal labeling quantification method to distinguish AFP-negative HCC from LC patients in a validation set (n = 64). n-glycopeptides 45-60 paraoxonase 1 Homo sapiens 64-68 33406838-7 2021 Our recently developed liquid chromatography-mass spectrometry methodology allowed us to identify LacdiNAc structural motifs on all occupied N-glycopeptides and polyLacNAc structures on six glycopeptides of the spike protein. n-glycopeptides 141-156 surface glycoprotein Severe acute respiratory syndrome coronavirus 2 211-216 30370771-0 2019 Differential Quantitative Determination of Site-Specific Intact N-Glycopeptides in Serum Haptoglobin between Hepatocellular Carcinoma and Cirrhosis Using LC-EThcD-MS/MS. n-glycopeptides 64-79 haptoglobin Homo sapiens 89-100 32892942-8 2020 Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 muL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. n-glycopeptides 111-126 N-glycanase 1 Homo sapiens 215-221 32892942-8 2020 Moreover, in the LC-MS/MS analysis of real biological sample, a total of 344 unique N-glycosites in 598 unique N-glycopeptides from 172 N-glycoproteins were identified from 2 muL human serum after deglycosylated by PNGase F, and 825 intact N-glycopeptides with different types of glycoform were detected when directly analyzed the N-glycopeptides enriched by PAM-OH HMS. n-glycopeptides 111-126 peptidylglycine alpha-amidating monooxygenase Homo sapiens 359-362 31517451-3 2019 The protocols include sample preparation of a 1:1 mixture of light (-CH3 )2 and heavy (-13 CD2 H)2 dimethylated intact N-glycopeptides from LO2 and HepG2 cells, RPLC-pentaHILIC 2DLC separation of the mixture, intact N-glycopeptide database search and identification using GPSeeker, and quantitation of differentially expressed intact N-glycopeptides using the quantitation module GPSeekerQuan. n-glycopeptides 119-134 CD2 molecule Homo sapiens 91-94 31393126-6 2019 A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time. n-glycopeptides 14-29 major royal jelly protein 1 Apis mellifera 126-131 31393126-6 2019 A total of 50 N-glycopeptides and 30 N-glycans have been site-specific glycosylation profiled in major royal jelly protein 1 (MRJP1) and MRJP2 of RJ for the first time. n-glycopeptides 14-29 major royal jelly protein 2 Apis mellifera 137-142 31079452-4 2019 Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions. n-glycopeptides 254-269 AXL receptor tyrosine kinase Homo sapiens 10-13 31079452-4 2019 Using the AXL receptor tyrosine kinase (AXL) as a model glycoprotein with multiple N-glycosylation sites, we show that those LCMS features that could be grouped into graph networks on the basis of glycan mass and retention time differences were actually N-glycopeptides with the same peptide backbone but different N-glycan compositions. n-glycopeptides 254-269 AXL receptor tyrosine kinase Homo sapiens 40-43 31079452-8 2019 GlycopeptideGraphMS detected more than 500 unique N-glycopeptides from AXL, triple the number found by a database search with Byonic software, and detected incorrect assignments due to a nonspecific protease cleavage. n-glycopeptides 50-65 AXL receptor tyrosine kinase Homo sapiens 71-74 32412768-3 2020 Herein, we have performed a comprehensive screening of site-specific N-glycopeptides in serum haptoglobin (Hp), a reporter molecule for aberrant glycosylation in HCC, to characterize glycopeptide markers for NASH-related HCCs. n-glycopeptides 69-84 haptoglobin Homo sapiens 94-105 32412768-6 2020 Receiver operating characteristic (ROC) curve analysis demonstrated that the N-glycopeptides at sites N184 and N241 bearing a monofucosylated tri-antennary glycan A3G3F1S3, had the best diagnostic performance in detection of early NASH HCC, area under the curve (AUC) = 0.733 and 0.775, respectively, whereas alpha-fetoprotein (AFP) had an AUC of 0.692. n-glycopeptides 77-92 alpha fetoprotein Homo sapiens 309-326 32412768-6 2020 Receiver operating characteristic (ROC) curve analysis demonstrated that the N-glycopeptides at sites N184 and N241 bearing a monofucosylated tri-antennary glycan A3G3F1S3, had the best diagnostic performance in detection of early NASH HCC, area under the curve (AUC) = 0.733 and 0.775, respectively, whereas alpha-fetoprotein (AFP) had an AUC of 0.692. n-glycopeptides 77-92 alpha fetoprotein Homo sapiens 328-331 32306330-4 2020 Recently, the recombinant Fbs1 derivative protein has been developed as a tool for comprehensive enrichment of N-glycopeptides. n-glycopeptides 111-126 F-box protein 2 Homo sapiens 26-30 31377859-6 2019 When using MagG@PEI@HA, 376 N-glycopeptides derived from 102 glycoproteins were identified, both from a 2 muL serum sample. n-glycopeptides 28-43 tripartite motif containing 37 Homo sapiens 106-109 30798070-4 2019 Using the high-temperature RPLC method, alpha2-6-linked sialylated N-glycopeptides were eluted first, followed by alpha2-3-linked isomers. n-glycopeptides 67-82 immunoglobulin binding protein 1 Homo sapiens 40-48 30370771-2 2019 Herein, we developed a workflow that involved advanced methodologies, the EThcD-MS/MS fragmentation method and data interpretation software, for differential analysis of the microheterogeneity of site-specific intact N-glycopeptides of serum haptoglobin between early hepatocellular carcinoma (HCC) and liver cirrhosis. n-glycopeptides 217-232 haptoglobin Homo sapiens 242-253 28534482-0 2017 An engineered high affinity Fbs1 carbohydrate binding protein for selective capture of N-glycans and N-glycopeptides. n-glycopeptides 101-116 fibrosin Homo sapiens 28-32 29557479-7 2018 We found that boronic acid chemistry in this study preferred to capture glycopeptides with high mannose glycans, ZIC-HILIC enriched most N-glycopeptides and did not show significant preference during enrichment and PGC was not suitable for separating glycopeptides with a long amino acid sequence. n-glycopeptides 137-152 zinc finger protein of the cerebellum 1 Mus musculus 113-116 29237727-6 2018 To demonstrate the precision of the approach, we also profiled N-glycopeptides from the mutant (xylt) of beta-1,2-xylosyltransferase, an enzyme in the N-glycan biosynthetic pathway. n-glycopeptides 63-78 beta-1,2-xylosyltransferase Arabidopsis thaliana 105-132 29806066-2 2018 In this work, we used separation involving hydrophilic interaction liquid chromatography using a superficially porous particle HALO penta-HILIC column with tandem mass spectrometric detection for the analysis of N-glycopeptides of hemopexin. n-glycopeptides 213-228 hemopexin Homo sapiens 232-241 29644550-0 2018 Distinctive and Complementary MS2 Fragmentation Characteristics for Identification of Sulfated Sialylated N-Glycopeptides by nanoLC-MS/MS Workflow. n-glycopeptides 106-121 MS2 Homo sapiens 30-33 29687791-0 2018 Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD). n-glycopeptides 51-66 pyruvate kinase M1/2 Homo sapiens 70-74 29502397-8 2018 A total of 67 N-glycopeptides were identified from the PSA pooled from the patients. n-glycopeptides 14-29 kallikrein related peptidase 3 Homo sapiens 55-58 27760464-6 2016 165 site-specific N-glycopeptides representative of all N-glycosylation sites were identified from AGP 1 and AGP 2 isoforms. n-glycopeptides 18-33 orosomucoid 1 Homo sapiens 99-104 27760464-6 2016 165 site-specific N-glycopeptides representative of all N-glycosylation sites were identified from AGP 1 and AGP 2 isoforms. n-glycopeptides 18-33 orosomucoid 2 Homo sapiens 109-114 25374123-3 2014 Here, we systematically analyzed the site-specific N-glycopeptides of vitronectin in human plasma by tandem mass spectrometry combined with immunoprecipitation and hydrophilic interaction liquid chromatography (HILIC) enrichment. n-glycopeptides 51-66 vitronectin Homo sapiens 70-81 27565792-5 2016 Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. n-glycopeptides 29-44 serpin family A member 3 Homo sapiens 112-136 27565792-5 2016 Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. n-glycopeptides 29-44 serpin family A member 1 Homo sapiens 138-157 27565792-5 2016 Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. n-glycopeptides 29-44 ceruloplasmin Homo sapiens 184-197 27565792-5 2016 Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. n-glycopeptides 29-44 orosomucoid 1 Homo sapiens 199-228 27565792-5 2016 Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. n-glycopeptides 29-44 alpha-2-macroglobulin Homo sapiens 230-251 27565792-5 2016 Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. n-glycopeptides 29-44 transferrin Homo sapiens 253-268 27565792-5 2016 Additionally, 37 fucosylated N-glycopeptides were newly identified from nine liver-secreted proteins, including alpha-1-antichymotrypsin, alpha-1-antitrypsin, alpha-2-HS-glycoprotein, ceruloplasmin, alpha-1-acid glycoprotein 1/2, alpha-2-macroglobulin, serotransferrin, and beta-2-glycoprotein 1. n-glycopeptides 29-44 apolipoprotein H Homo sapiens 274-295 27591658-1 2016 In this work, we describe a multivariate data analysis approach for data exploration and classification of the complex and large data sets generated to study the alteration of human transferrin (Tf) N-glycopeptides in patients with congenital disorders of glycosylation (CDG). n-glycopeptides 199-214 transferrin Homo sapiens 182-193 27591658-1 2016 In this work, we describe a multivariate data analysis approach for data exploration and classification of the complex and large data sets generated to study the alteration of human transferrin (Tf) N-glycopeptides in patients with congenital disorders of glycosylation (CDG). n-glycopeptides 199-214 transferrin Homo sapiens 195-197 27519006-3 2016 Detailed site-specific reference glycoprofiles of purified basigin were manually established using ion-trap CID-MS/MS and high-resolution Q-Exactive Orbitrap HCD-MS/MS of tryptic N-glycopeptides and released N-glycans. n-glycopeptides 179-194 basigin (Ok blood group) Homo sapiens 59-66 27139140-0 2016 pGlyco: a pipeline for the identification of intact N-glycopeptides by using HCD- and CID-MS/MS and MS3. n-glycopeptides 52-67 MS3 Homo sapiens 100-103 26700048-4 2016 As a result of their higher hydrophilicity N-glycopeptides comprising short peptide backbones are preferably accumulated by the ZIC-HILIC-based separation procedure. n-glycopeptides 43-58 Zic family member 1 Homo sapiens 128-131 25374123-4 2014 Vitronectin was purified with immunoprecipitation by monoclonal antibody from plasma and digested to tryptic N-glycopeptides.Then, enrichment with HILIC materials was used and followed by analysis with nano-LC/MS/MS. n-glycopeptides 109-124 vitronectin Homo sapiens 0-11 25374123-8 2014 As a result, a total of 17 site-specific N-glycopeptides were completely identified in all of the three N-glycosylation sites of vitronectin in human plasma, including 12 N-glycopeptides first reported. n-glycopeptides 41-56 vitronectin Homo sapiens 129-140 25374123-8 2014 As a result, a total of 17 site-specific N-glycopeptides were completely identified in all of the three N-glycosylation sites of vitronectin in human plasma, including 12 N-glycopeptides first reported. n-glycopeptides 171-186 vitronectin Homo sapiens 129-140 24668066-1 2014 Conventional N-glycoproteome analysis usually applies C18 reversed-phase (RP) adsorbent for sample purification, which will lead to unavoidable sample loss due to the high hydrophilicity of N-glycopeptides. n-glycopeptides 190-205 Bardet-Biedl syndrome 9 Homo sapiens 54-57 24668066-3 2014 It was observed that the small hydrophilic N-glycopeptides that cannot retain onto C18 adsorbent can be captured by the graphitized carbon, while the large hydrophobic N-glycopeptides that cannot retain onto the graphitized carbon can be feasibly captured by the C18 adsorbent. n-glycopeptides 43-58 Bardet-Biedl syndrome 9 Homo sapiens 83-86 24668066-4 2014 Comparing with sample purification by using C18 adsorbent only, 28.5 % more N-glycopeptides were identified by combining both C18 and PGC adsorbents. n-glycopeptides 76-91 Bardet-Biedl syndrome 9 Homo sapiens 44-47 24668066-4 2014 Comparing with sample purification by using C18 adsorbent only, 28.5 % more N-glycopeptides were identified by combining both C18 and PGC adsorbents. n-glycopeptides 76-91 Bardet-Biedl syndrome 9 Homo sapiens 126-129 22223312-3 2012 The experimental data showed that the microcolumn could efficiently and reproducibly isolate N-glycopeptides from enzymatic mixtures of ovalbumin, fetuin, haptoglobin and human plasma. n-glycopeptides 93-108 haptoglobin Homo sapiens 155-166 23028207-2 2012 Herein, the detailed N-glycosylation pattern of human serum alpha-2-macroglobulin was studied using an integrative approach, including permethylation of N-glycans, collision induced dissociation (CID) and electron transfer dissociation (ETD) of chymotryptic N-glycopeptides, and partial deglycosylation of chymotryptic N-glycopeptides with endo-beta-N-acetylglucosaminidase F3 (Endo F3). n-glycopeptides 258-273 alpha-2-macroglobulin Homo sapiens 60-81 23028207-2 2012 Herein, the detailed N-glycosylation pattern of human serum alpha-2-macroglobulin was studied using an integrative approach, including permethylation of N-glycans, collision induced dissociation (CID) and electron transfer dissociation (ETD) of chymotryptic N-glycopeptides, and partial deglycosylation of chymotryptic N-glycopeptides with endo-beta-N-acetylglucosaminidase F3 (Endo F3). n-glycopeptides 319-334 alpha-2-macroglobulin Homo sapiens 60-81