PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 11516162-4 2001 Using Ni(2+)-immobilized agarose affinity chromatography, the recombinant mouse DAF modules with their 6x His tags could be one-step isolated to SDS-PAGE purity. Sepharose 25-32 CD55 molecule, decay accelerating factor for complement Mus musculus 80-83 11570502-6 2001 As an example, we demonstrate that more than 95% of the human transferrin receptor bound to a transferrin-sepharose ligand affinity column can be eluted with full binding activity at KSCN concentrations between 232 and 414 nM, whereas elution with urea is not suitable to purify fully functional protein. Sepharose 106-115 transferrin Homo sapiens 62-73 11570502-6 2001 As an example, we demonstrate that more than 95% of the human transferrin receptor bound to a transferrin-sepharose ligand affinity column can be eluted with full binding activity at KSCN concentrations between 232 and 414 nM, whereas elution with urea is not suitable to purify fully functional protein. Sepharose 106-115 transferrin Homo sapiens 94-105 11695234-6 2001 Agarose gel electrophoresis of DNAs extracted from the VP, SV, CG and Ep of +/+, lpr/lpr and gld/gld mice on day 4 after castration showed a ladder pattern. Sepharose 0-7 Fas (TNF receptor superfamily member 6) Mus musculus 81-84 11695234-6 2001 Agarose gel electrophoresis of DNAs extracted from the VP, SV, CG and Ep of +/+, lpr/lpr and gld/gld mice on day 4 after castration showed a ladder pattern. Sepharose 0-7 Fas (TNF receptor superfamily member 6) Mus musculus 85-88 11580191-1 2001 By affinity chromatography with Sepharose coupled to 2"-deoxy-1-beta-D-ribofuranosyl-N4-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated. Sepharose 32-41 deoxycytidine kinase Mus musculus 108-128 11561721-10 2001 The quantitative affinity chromatographic studies showed that the influence of GTP on the binding of GDH isoproteins to DEPC-Sepharose was significantly distinct for the two GDH isoproteins. Sepharose 125-134 glutamate dehydrogenase 1, mitochondrial Bos taurus 101-104 11561721-10 2001 The quantitative affinity chromatographic studies showed that the influence of GTP on the binding of GDH isoproteins to DEPC-Sepharose was significantly distinct for the two GDH isoproteins. Sepharose 125-134 glutamate dehydrogenase 1, mitochondrial Bos taurus 174-177 11561721-11 2001 GDH I was more sensitively affected by GTP than GDH II in the binding affinity for DEPC-Sepharose. Sepharose 88-97 glutamate dehydrogenase 1, mitochondrial Bos taurus 0-3 11561721-11 2001 GDH I was more sensitively affected by GTP than GDH II in the binding affinity for DEPC-Sepharose. Sepharose 88-97 glutamate dehydrogenase 1, mitochondrial Bos taurus 48-51 11761848-3 2001 The coding sequence of MyoD in PEMMBC2 beta 5 was confirmed by agarose gel electrophoresis and DNA sequence analysis. Sepharose 63-70 myogenic differentiation 1 Mus musculus 23-27 11761848-5 2001 The pcDNA3/MyoD, digested with restriction enzymes, was found to contain the MyoD cDNA sequence by agarose gel electrophoresis analysis. Sepharose 99-106 myogenic differentiation 1 Mus musculus 11-15 11761848-5 2001 The pcDNA3/MyoD, digested with restriction enzymes, was found to contain the MyoD cDNA sequence by agarose gel electrophoresis analysis. Sepharose 99-106 myogenic differentiation 1 Mus musculus 77-81 11397808-5 2001 The permeabilization of Env-expressing cells was also triggered by CD4.co-receptor complexes attached to Protein G-Sepharose beads in the absence of target cells. Sepharose 115-124 endogenous retrovirus group K member 20 Homo sapiens 24-27 11397808-5 2001 The permeabilization of Env-expressing cells was also triggered by CD4.co-receptor complexes attached to Protein G-Sepharose beads in the absence of target cells. Sepharose 115-124 CD4 molecule Homo sapiens 67-70 11478777-0 2001 Dynamic compression inhibits the synthesis of nitric oxide and PGE(2) by IL-1beta-stimulated chondrocytes cultured in agarose constructs. Sepharose 118-125 interleukin 1 beta Homo sapiens 73-81 11749844-7 2001 After incubation of K562 cells with CFP-1 300 mg/L for 5 d, morphological changes of typical apoptosis were observed and agarose gel electrophoresis of DNA revealed "ladder" pattern. Sepharose 121-128 CXXC finger protein 1 Homo sapiens 36-41 11461121-9 2001 The appearance of the fragmented chromatin visualized by DAPI staining, and DNA fragments seen on an agarose gel, coincided with the decrease in ERK1/2 activation and concomitant increase of the level of active p38 MAPK. Sepharose 101-108 mitogen-activated protein kinase 3 Homo sapiens 145-151 11461121-9 2001 The appearance of the fragmented chromatin visualized by DAPI staining, and DNA fragments seen on an agarose gel, coincided with the decrease in ERK1/2 activation and concomitant increase of the level of active p38 MAPK. Sepharose 101-108 mitogen-activated protein kinase 1 Homo sapiens 211-214 11563677-2 2001 We attempted to isolate the putative receptor protein p62 from cell lysates of the human B cell line Raji by affinity chromatography using sepharose-columns which were conjugated with three different peptides of the C-domain respectively. Sepharose 139-148 nucleoporin 62 Homo sapiens 54-57 11563677-3 2001 A protein of 62 kDa was isolated by peptide (NP2)-sepharose-column, while none of the proteins was identified in eluates of the other two overlapped peptides of C-domain, indicating that HIV-1 gp41 by the region aa635-664 of C-domain binds to the putative receptor protein p62. Sepharose 50-59 neuropilin 2 Homo sapiens 45-48 11511796-1 2001 Recombinant mouse tryptophan hydroxylase (TPH) was expressed in Escherichia coli, using a bacterial expression vector and has been purified to homogeneity by sonication followed by Sepharose 4B column chromatography and native slab gel electrophoresis. Sepharose 181-190 tryptophan hydroxylase 1 Mus musculus 18-40 11511796-1 2001 Recombinant mouse tryptophan hydroxylase (TPH) was expressed in Escherichia coli, using a bacterial expression vector and has been purified to homogeneity by sonication followed by Sepharose 4B column chromatography and native slab gel electrophoresis. Sepharose 181-190 tryptophan hydroxylase 1 Mus musculus 42-45 11520897-10 2001 The F3-Fc coated Sepharose beads precipitated RPTPzeta/beta from SC lysates. Sepharose 17-26 protein tyrosine phosphatase receptor type Z1 Homo sapiens 46-59 11444983-9 2001 PS1 was specifically absorbed to pepstatin-agarose, with an optimal pH of 6. Sepharose 43-50 presenilin 1 Homo sapiens 0-3 11437376-7 2001 In agarose gel electrophoresis, AxCAEC-SOD decreased the negative charge of oxidized LDL by 50% and suppressed fragmentation of apolipoprotein B. Sepharose 3-10 apolipoprotein B Homo sapiens 128-144 11465712-10 2001 Anchorage-independent growth and the reexpression of COL2A1 in agarose culture were insufficient to predict cartilage formation in vivo. Sepharose 63-70 collagen type II alpha 1 chain Homo sapiens 53-59 11433424-8 2001 Hyaluronan-sepharose chromatography and cetylpiridinium chloride precipitation confirmed the hyaluronan-binding capacity of RHAMM forms. Sepharose 11-20 hyaluronan-mediated motility receptor Rattus norvegicus 124-129 11413323-4 2001 IgG1, IgG2, and IgG3 were enriched from HIVIG by using protein A-Sepharose. Sepharose 65-74 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 16-20 11423751-5 2001 IgA was isolated with affinity chromatography using cyanogen bromide activated Sepharose 4B coupled to sheep antihuman IgA antiserum. Sepharose 79-91 immunoglobulin heavy variable 4-38-2-like Homo sapiens 0-3 11467894-9 2001 RESULTS: IL-1beta suppressed aggrecan synthesis by chondrocytes in agarose. Sepharose 67-74 interleukin 1 beta Homo sapiens 9-17 11465839-6 2001 Colostral IgA was isolated by affinity chromatography in Sepharose anti-human alpha chain column. Sepharose 57-66 CD79a molecule Homo sapiens 10-13 11520413-2 2001 MATERIALS AND METHODS: A1PI was purified from Cohn fraction IV-1,4 using ethanol precipitation and Q-Sepharose chromatography. Sepharose 101-110 serpin family A member 1 Homo sapiens 23-27 11312263-4 2001 AHNAK binds to S100B-Sepharose beads and is also recovered in anti-S100B immunoprecipitates in a strict Ca(2+)- and Zn(2+)-dependent manner. Sepharose 21-30 AHNAK nucleoprotein Rattus norvegicus 0-5 11312263-4 2001 AHNAK binds to S100B-Sepharose beads and is also recovered in anti-S100B immunoprecipitates in a strict Ca(2+)- and Zn(2+)-dependent manner. Sepharose 21-30 S100 calcium binding protein B Rattus norvegicus 15-20 11320080-7 2001 The NCLK from the eluted column fractions bound to PP1-specific microcystin-Sepharose and glutathione S-transferase (GST)-I-2-coated glutathione-agarose beads. Sepharose 76-85 inorganic pyrophosphatase 1 Homo sapiens 51-54 11320080-8 2001 Similarly, PP1 from the eluted column fractions was pulled down with GST-Cdk5-coated glutathione-agarose beads. Sepharose 97-104 inorganic pyrophosphatase 1 Homo sapiens 11-14 11320080-8 2001 Similarly, PP1 from the eluted column fractions was pulled down with GST-Cdk5-coated glutathione-agarose beads. Sepharose 97-104 cyclin dependent kinase 5 Bos taurus 73-77 11304529-5 2001 Also, GST.Tat prevents the binding of [(3)H]heparin to GST.Tat immobilized to glutathione-agarose beads. Sepharose 90-97 tyrosine aminotransferase Homo sapiens 10-13 11304529-5 2001 Also, GST.Tat prevents the binding of [(3)H]heparin to GST.Tat immobilized to glutathione-agarose beads. Sepharose 90-97 tyrosine aminotransferase Homo sapiens 59-62 11416206-10 2001 The enhanced translocation efficiency of TAP1/T2MT1C relative to TAP2/T1MT2C complexes correlates with enhanced binding of the TAP1 NBD-containing constructs to ATP-agarose beads. Sepharose 165-172 transporter 1, ATP binding cassette subfamily B member Homo sapiens 41-45 11416206-10 2001 The enhanced translocation efficiency of TAP1/T2MT1C relative to TAP2/T1MT2C complexes correlates with enhanced binding of the TAP1 NBD-containing constructs to ATP-agarose beads. Sepharose 165-172 transporter 2, ATP binding cassette subfamily B member Homo sapiens 65-69 11416206-10 2001 The enhanced translocation efficiency of TAP1/T2MT1C relative to TAP2/T1MT2C complexes correlates with enhanced binding of the TAP1 NBD-containing constructs to ATP-agarose beads. Sepharose 165-172 transporter 1, ATP binding cassette subfamily B member Homo sapiens 127-131 11390517-4 2001 Ig using an affinity matrix consisting of a synthetic peptide corresponding to the N-terminus of CCR5 coupled to Sepharose. Sepharose 113-122 C-C motif chemokine receptor 5 Homo sapiens 97-101 11397124-11 2001 When the serum was passed over a gelatin-Sepharose column, which binds numerous proteins including fibronectin, the serum effect was lost. Sepharose 41-50 fibronectin 1 Homo sapiens 99-110 11353864-4 2001 Purification of GST-RyR3 was achieved by affinity chromatography by using glutathione-Sepharose. Sepharose 86-95 glutathione S-transferase kappa 1 Homo sapiens 16-19 11410294-4 2001 In this study, the pathway of hPAH degradation is addressed with focus on its conjugation with polyubiquitin chains catalysed by the ubiquitin-conjugating enzyme system (E1, E2, E3) isolated from rat liver by covalent affinity chromatography on ubiquitin-Sepharose. Sepharose 255-264 carboxylesterase 1C Rattus norvegicus 170-180 11475150-3 2001 Von Willebrand"s factor (VWF) multimers were assayed with high- and low-resolution sodium dodecyl sulfate (SDS) agarose gels. Sepharose 112-119 von Willebrand factor Homo sapiens 0-23 11475150-3 2001 Von Willebrand"s factor (VWF) multimers were assayed with high- and low-resolution sodium dodecyl sulfate (SDS) agarose gels. Sepharose 112-119 von Willebrand factor Homo sapiens 25-28 11278906-4 2001 After cell-surface biotinylation, isoelectric focusing of membrane fraction, avidin-agarose extraction of biotinylated proteins, and SDS-polyacrylamide gel electrophoresis, EWS protein was identified by matrix-assisted laser desorption ionization and nanoelectrospray tandem mass spectrometry of in-gel-digested peptides. Sepharose 84-91 EWS RNA binding protein 1 Homo sapiens 173-176 11409126-7 2001 Upon fractionation of MMP by gelatin-Sepharose affinity chromatography it was found that the gelatin-unbound collagenases are exclusively responsible for DNP-S degrading activity. Sepharose 37-46 matrix metallopeptidase 1 Homo sapiens 22-25 11433776-5 2001 alpha 2-Macroglobulin (alpha 2M) was isolated from the human blood plasma by affinity chromatography on a column containing Asp-hemolysin coupled to Sepharose. Sepharose 149-158 alpha-2-macroglobulin Homo sapiens 0-21 11433776-5 2001 alpha 2-Macroglobulin (alpha 2M) was isolated from the human blood plasma by affinity chromatography on a column containing Asp-hemolysin coupled to Sepharose. Sepharose 149-158 alpha-2-macroglobulin Homo sapiens 23-31 11353864-4 2001 Purification of GST-RyR3 was achieved by affinity chromatography by using glutathione-Sepharose. Sepharose 86-95 ryanodine receptor 3 Homo sapiens 20-24 11278648-2 2001 Previous studies have demonstrated that the estrogen receptor binds to calmodulin-Sepharose in vitro. Sepharose 82-91 estrogen receptor 1 Homo sapiens 44-61 11145957-5 2001 This possibility was tested using affinity chromatography by applying synthetic fragments of pro-IAPP to heparin-agarose and heparan sulfate-Sepharose. Sepharose 113-120 islet amyloid polypeptide Homo sapiens 97-101 11145957-5 2001 This possibility was tested using affinity chromatography by applying synthetic fragments of pro-IAPP to heparin-agarose and heparan sulfate-Sepharose. Sepharose 141-150 islet amyloid polypeptide Homo sapiens 97-101 11145957-6 2001 An N-terminal human pro-IAPP fragment (residues 1-30) was retained by both heparin-agarose and heparan sulfate-Sepharose, eluting at 0.18 m NaCl at pH 7.5. Sepharose 83-90 islet amyloid polypeptide Homo sapiens 24-28 11145957-6 2001 An N-terminal human pro-IAPP fragment (residues 1-30) was retained by both heparin-agarose and heparan sulfate-Sepharose, eluting at 0.18 m NaCl at pH 7.5. Sepharose 111-120 islet amyloid polypeptide Homo sapiens 24-28 11278648-2 2001 Previous studies have demonstrated that the estrogen receptor binds to calmodulin-Sepharose in vitro. Sepharose 82-91 calmodulin 1 Homo sapiens 71-81 11311128-4 2001 In the present paper we report the direct interaction of TRP4 and calmodulin (CaM) by: (1) retention of in vitro translated TRP4 and of TRP4 protein solubilized from bovine adrenal cortex by CaM-Sepharose in the presence of Ca(2+), and (2) TRP4-glutathione S-transferase pull-down experiments. Sepharose 195-204 transient receptor potential cation channel subfamily C member 4 Homo sapiens 57-61 11350927-6 2001 Both GD and Snk bind to heparin-Sepharose, providing a link between the pipe-defined ventral prepattern and the protease cascade. Sepharose 32-41 snake Drosophila melanogaster 12-15 11311128-4 2001 In the present paper we report the direct interaction of TRP4 and calmodulin (CaM) by: (1) retention of in vitro translated TRP4 and of TRP4 protein solubilized from bovine adrenal cortex by CaM-Sepharose in the presence of Ca(2+), and (2) TRP4-glutathione S-transferase pull-down experiments. Sepharose 195-204 calmodulin Bos taurus 66-76 11311128-4 2001 In the present paper we report the direct interaction of TRP4 and calmodulin (CaM) by: (1) retention of in vitro translated TRP4 and of TRP4 protein solubilized from bovine adrenal cortex by CaM-Sepharose in the presence of Ca(2+), and (2) TRP4-glutathione S-transferase pull-down experiments. Sepharose 195-204 calmodulin Bos taurus 78-81 11311147-7 2001 Nevertheless, suramin and NF307 only blocked the binding of Gbetagamma and RyR1 to calmodulin-Sepharose. Sepharose 94-103 ryanodine receptor 1 Oryctolagus cuniculus 75-79 11516152-5 2001 The behaviour of the myristoylated form of StCDPK1 during its purification on a phenyl-Sepharose column mimics that of the endogenous potato enzyme suggesting that this modification occurs in vivo. Sepharose 87-96 calcium-dependent protein kinase Solanum tuberosum 43-50 11386872-6 2001 hCTRB is further purified by adsorption from the top phase to the cation exchanger SP Sepharose Big Beads and elution in a salt step. Sepharose 86-95 chymotrypsinogen B1 Homo sapiens 0-5 11402501-11 2001 Furthermore, fH and factor I (fI)-mediated cleavage of agarose bound C3b into iC3b was decreased in the presence of pepAred. Sepharose 55-62 complement factor H Homo sapiens 13-15 11402501-11 2001 Furthermore, fH and factor I (fI)-mediated cleavage of agarose bound C3b into iC3b was decreased in the presence of pepAred. Sepharose 55-62 complement C3 Homo sapiens 69-72 11377982-2 2001 Recombinant human dehydroepiandrosterone sulfotransferase (DHEA-ST) expressed in glutathione sulfotransferase fusion form in E. coli was purified using glutathione sepharose 4B affinity adsorption chromatography, a Factor Xa cleavage step, and Q-sepharose fast flow column chromatography. Sepharose 164-173 sulfotransferase family 2A member 1 Homo sapiens 18-57 11377982-2 2001 Recombinant human dehydroepiandrosterone sulfotransferase (DHEA-ST) expressed in glutathione sulfotransferase fusion form in E. coli was purified using glutathione sepharose 4B affinity adsorption chromatography, a Factor Xa cleavage step, and Q-sepharose fast flow column chromatography. Sepharose 164-173 sulfotransferase family 2A member 1 Homo sapiens 59-66 11377982-2 2001 Recombinant human dehydroepiandrosterone sulfotransferase (DHEA-ST) expressed in glutathione sulfotransferase fusion form in E. coli was purified using glutathione sepharose 4B affinity adsorption chromatography, a Factor Xa cleavage step, and Q-sepharose fast flow column chromatography. Sepharose 246-255 sulfotransferase family 2A member 1 Homo sapiens 18-57 11377982-2 2001 Recombinant human dehydroepiandrosterone sulfotransferase (DHEA-ST) expressed in glutathione sulfotransferase fusion form in E. coli was purified using glutathione sepharose 4B affinity adsorption chromatography, a Factor Xa cleavage step, and Q-sepharose fast flow column chromatography. Sepharose 246-255 sulfotransferase family 2A member 1 Homo sapiens 59-66 11311147-7 2001 Nevertheless, suramin and NF307 only blocked the binding of Gbetagamma and RyR1 to calmodulin-Sepharose. Sepharose 94-103 calmodulin Oryctolagus cuniculus 83-93 11288141-6 2001 Apoptotic-associated oligonucleosomal-sized DNA fragmentation on agarose gels was detected from 6 to 72 hr after exposure to TNF-alpha. Sepharose 65-72 tumor necrosis factor Homo sapiens 125-134 11319880-13 2001 This was confirmed further by 7-methyl-GTP Sepharose isolation of eIF-4E with which 4E-BP1 coprecipitates. Sepharose 43-52 eukaryotic translation initiation factor 4E Rattus norvegicus 66-72 11319880-13 2001 This was confirmed further by 7-methyl-GTP Sepharose isolation of eIF-4E with which 4E-BP1 coprecipitates. Sepharose 43-52 eukaryotic translation initiation factor 4E binding protein 1 Rattus norvegicus 84-90 11393956-3 2001 The resulting Sal I-Xba I fragment (1.24 kb) which contains the full length of human BMP-2 cDNA was separated on agarose gel and ligated into eukaryotic expression vector pcDNA3 digested with XhoI and XbaI. Sepharose 113-120 bone morphogenetic protein 2 Homo sapiens 85-90 11393956-6 2001 RESULTS: The two fragments digested from recombinant pcDNA3-hBMP-2 plasmid by EcoR I and Xba I represented 1.3 kb and 5.38 kb respectively by agarose electrophoresis, meanwhile the Xho I site was disappeared in pcDNA3-hBMP-2 indicating the successful construction of recombinant pcDNA3-hBMP-2 plasmid. Sepharose 142-149 bone morphogenetic protein 2 Homo sapiens 60-66 11336784-6 2001 The colony size of this cell line in agarose suspension cultures was reduced to 20-40% of control, when exposed to Wnt-1, 3a, 4, 7a, and 7b for 14 days. Sepharose 37-44 Wnt family member 1 Rattus norvegicus 115-120 11258898-6 2001 Activated MAPK immunoprecipitates of nuclei from T(4)-treated cells accumulated p53 in a time-dependent manner; T(4) and T(4)-agarose were more effective than T(3). Sepharose 126-133 tumor protein p53 Homo sapiens 80-83 11278417-3 2001 Affinity chromatography was utilized to purify UPase from colon 26 and NIH 3T3 cells using the uridine phosphorylase inhibitor 5"-amino benzylacyclouridine linked to an agarose matrix. Sepharose 169-176 uridine phosphorylase 1 Mus musculus 47-52 11247938-0 2001 Mechanical compression influences intracellular Ca2+ signaling in chondrocytes seeded in agarose constructs. Sepharose 89-96 carbonic anhydrase 2 Bos taurus 48-51 11260419-6 2001 METHODS: Agarose-immobilized murine anti-human beta2m monoclonal antibodies were used in a Vortex Flow Plasmapheretic Reactor (VFPR) to remove donor baseline and controlled amounts of recombinant beta2m from human blood in vitro. Sepharose 9-16 beta-2-microglobulin Homo sapiens 47-53 11260419-6 2001 METHODS: Agarose-immobilized murine anti-human beta2m monoclonal antibodies were used in a Vortex Flow Plasmapheretic Reactor (VFPR) to remove donor baseline and controlled amounts of recombinant beta2m from human blood in vitro. Sepharose 9-16 beta-2-microglobulin Homo sapiens 196-202 11495355-3 2001 In the present study we describe the affi-gel blue-nonbinding phosphatases which were separated into seven different phosphatase activities, designated P5-P11 by poly-(L-lysine)-agarose and aminohexyl Sepharose 4B chromatographies. Sepharose 201-210 S100 calcium binding protein A10 Homo sapiens 155-158 11281725-3 2001 In order to sort out functional Ets-1-binding sites among those present in gene promoters, we constructed an expression vector and designed a purification protocol for the production of the 440-amino-acid form of mouse Ets-1, based on heparin-Sepharose affinity and anion-exchange chromatographies. Sepharose 243-252 E26 avian leukemia oncogene 1, 5' domain Mus musculus 219-224 11286567-5 2001 Quantitative studies of LDH by agarose gel electrophoresis and Northern analysis in patients concluded that LDH-A(4)isozyme was increased in pre-eclampsia. Sepharose 31-38 lactate dehydrogenase A Homo sapiens 24-27 12905882-2 2001 METHOD: Chemically synthesized hEGF gene was expressed in Yeast Pichia pastoris and the secretory hEGF was purified by Phenysepharose 6 Fast Flow(high sub), Q-sepharose High Performance, and Superdex 30 chromatography, and its characters were studied by respective methods. Sepharose 124-133 epidermal growth factor Homo sapiens 31-35 12905882-2 2001 METHOD: Chemically synthesized hEGF gene was expressed in Yeast Pichia pastoris and the secretory hEGF was purified by Phenysepharose 6 Fast Flow(high sub), Q-sepharose High Performance, and Superdex 30 chromatography, and its characters were studied by respective methods. Sepharose 124-133 epidermal growth factor Homo sapiens 98-102 11098058-10 2001 When full-length collagen XIV polypeptides that either contain or lack the first F3 domain are tested on heparin-Sepharose, a pronounced difference in their relative affinity is observed. Sepharose 113-122 collagen type XIV alpha 1 chain Gallus gallus 17-29 11318608-10 2001 Our data show that DENTT mRNA induction by TGF-beta1 correlates with induction of TGF-beta1 mRNA, induction of extracellular matrix gene expression, and inhibition of colony formation in soft agarose in TGF-beta1 responsive NSCLC cells when exposed to TGF-beta1. Sepharose 192-199 TSPY like 2 Homo sapiens 19-24 11318608-10 2001 Our data show that DENTT mRNA induction by TGF-beta1 correlates with induction of TGF-beta1 mRNA, induction of extracellular matrix gene expression, and inhibition of colony formation in soft agarose in TGF-beta1 responsive NSCLC cells when exposed to TGF-beta1. Sepharose 192-199 transforming growth factor beta 1 Homo sapiens 43-52 11401532-3 2001 The preS1 was purified to near homogeneity from bacterially expressed glutathione S-transferase (GST)-preS1 fusion protein by two-step purification, affinity chromatography on glutathione-agarose column, and cation-exchange chromatography on Mono S column. Sepharose 188-195 large envelope protein;middle envelope protein;small envelope protein Hepatitis B virus 4-9 11308323-3 2001 Glycosylated bovine beta-casein (L70S/P71S) expressed in P. pastoris was purified to homogeneity by the combination of ammonium sulfate fractionation, Concanavalin A--Sepharose affinity column, and Mono Q anion-exchange FPLC. Sepharose 167-176 casein beta Bos taurus 20-31 11254726-6 2001 Stimulation of neutrophils by these agonists decreases the association of SAM68 with Sepharose-conjugated poly-U beads. Sepharose 85-94 KH RNA binding domain containing, signal transduction associated 1 Homo sapiens 74-79 11248329-2 2001 For each clone, we found a significant correlation between the reduction in 3[H]-thymidine incorporation and the reduction in CD25 expression (r=0.701, P<0.05) following treatment with 10(-5) M ATRA for 48 h. Agarose gel electrophoresis revealed DNA fragmentation of the cell lines treated with ATRA, indicative of apoptosis. Sepharose 212-219 interleukin 2 receptor subunit alpha Homo sapiens 126-130 11294894-5 2001 Here, we demonstrate that layilin-Fc fusion protein binds to hyaluronan immobilized to Sepharose. Sepharose 87-96 layilin Homo sapiens 26-33 11099496-3 2001 Agarose bead-coated phosphorylated ITIM peptides (pITIMs) bind in vitro the single-SH2 inositol 5-phosphatases (SHIP1 and SHIP2) and the two-SH2 protein tyrosine phosphatases (SHP-1 and SHP-2). Sepharose 0-7 inositol polyphosphate-5-phosphatase D Homo sapiens 112-117 11248021-6 2001 Third, GTP-p67(PHOX) bound to glutathione agarose is able to pull down cytochrome b(558.) Sepharose 42-49 CD33 molecule Homo sapiens 11-14 11248021-6 2001 Third, GTP-p67(PHOX) bound to glutathione agarose is able to pull down cytochrome b(558.) Sepharose 42-49 mitochondrially encoded cytochrome b Homo sapiens 71-83 11243846-9 2001 The receptor protein was purified from spleen and thymic lymphocytes using protein A agarose that precipitated material complexed to alphaN-MC5-R. Sepharose 85-92 melanocortin 5 receptor Rattus norvegicus 140-145 11226885-2 2001 This factor was immunologically identified as the cross-linked homodimer of S19 ribosomal protein (RP S19), since the majority of the chemotactic activity was absorbed by both anti--RP S19 rabbit antibodies and an anti--isopeptide bond monoclonal antibody immobilized on agarose beads. Sepharose 271-278 ribosomal protein S19 Homo sapiens 76-105 11226885-2 2001 This factor was immunologically identified as the cross-linked homodimer of S19 ribosomal protein (RP S19), since the majority of the chemotactic activity was absorbed by both anti--RP S19 rabbit antibodies and an anti--isopeptide bond monoclonal antibody immobilized on agarose beads. Sepharose 271-278 ribosomal protein S19 Homo sapiens 99-105 11207269-3 2001 To identify this tyrosine phosphorylated p95 component, we first purified it by affinity chromatography on anti-phosphotyrosine mAb covalently linked to Sepharose 4B, followed by polyacrylamide gel electrophoresis. Sepharose 153-162 nibrin Homo sapiens 41-44 11355444-8 2001 RESULTS: Recombinant gp120-CH-Sepharose chromatography of one subject"s parotid saliva revealed specific binding of human parotid basic proline-rich proteins, most prominently one with an apparent molecular weight of 37 kDa. Sepharose 30-39 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 21-26 11237688-4 2001 Inclusion bodies of expressed rat MMP-12 catalytic domain were denatured and refolded using a new method, and then affinity purified to near homogeneity with zinc-chelating Sepharose. Sepharose 173-182 matrix metallopeptidase 12 Rattus norvegicus 34-40 11237690-1 2001 Glucose-6-phosphate dehydrogenase was purified from human placenta using DEAE-Sepharose fast flow, 2",5"-ADP Sepharose 4B column chromatography, and chromatofocusing on PBE 94 with PB 74. Sepharose 78-87 glucose-6-phosphate dehydrogenase Homo sapiens 0-33 11248059-9 2001 In vivo association of D-AKAP2 with PKA in mouse brain was demonstrated by using cAMP agarose pull-down assay. Sepharose 86-93 A kinase (PRKA) anchor protein 10 Mus musculus 23-30 11171108-4 2001 Anhydrothrombin retained affinity for various natural substrates of thrombin, including fibrinogen, factor VIII, factor XIII and protein C. In addition, these proteins were bound to anhydrothrombin-agarose in a reversible manner. Sepharose 198-205 coagulation factor II, thrombin Homo sapiens 7-15 11171108-4 2001 Anhydrothrombin retained affinity for various natural substrates of thrombin, including fibrinogen, factor VIII, factor XIII and protein C. In addition, these proteins were bound to anhydrothrombin-agarose in a reversible manner. Sepharose 198-205 fibrinogen beta chain Homo sapiens 88-98 11305322-1 2001 PSGL-1, a specific ligand for P-, E- and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin-agarose and P- or E-selectin-agarose chromatography. Sepharose 158-165 selectin P ligand Homo sapiens 0-6 11305322-1 2001 PSGL-1, a specific ligand for P-, E- and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin-agarose and P- or E-selectin-agarose chromatography. Sepharose 187-194 selectin P ligand Homo sapiens 0-6 11254757-5 2001 Further characterization of the GPI-PLD-containing lipoproteins by gel-filtration chromatography and nondenaturing polyacrylamide and agarose gel electrophoresis revealed that these apoA-I-containing particles/complexes were small (8 nm) and migrated with pre-beta particles on agarose electrophoresis. Sepharose 134-141 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 32-39 11254757-5 2001 Further characterization of the GPI-PLD-containing lipoproteins by gel-filtration chromatography and nondenaturing polyacrylamide and agarose gel electrophoresis revealed that these apoA-I-containing particles/complexes were small (8 nm) and migrated with pre-beta particles on agarose electrophoresis. Sepharose 134-141 apolipoprotein A1 Homo sapiens 182-188 11254757-5 2001 Further characterization of the GPI-PLD-containing lipoproteins by gel-filtration chromatography and nondenaturing polyacrylamide and agarose gel electrophoresis revealed that these apoA-I-containing particles/complexes were small (8 nm) and migrated with pre-beta particles on agarose electrophoresis. Sepharose 278-285 apolipoprotein A1 Homo sapiens 182-188 11222776-7 2001 Using western blot analysis of GH3 nuclear proteins that bind to heparin-Sepharose, we have shown that Ets-1 and GABP, which are MAP kinase substrates, co-purify with complex A, and supershift analysis with specific antisera revealed that complex A contains Ets-1, GABPalpha and GABPbeta1. Sepharose 73-82 ETS proto-oncogene 1, transcription factor Rattus norvegicus 103-108 11099496-3 2001 Agarose bead-coated phosphorylated ITIM peptides (pITIMs) bind in vitro the single-SH2 inositol 5-phosphatases (SHIP1 and SHIP2) and the two-SH2 protein tyrosine phosphatases (SHP-1 and SHP-2). Sepharose 0-7 inositol polyphosphate phosphatase like 1 Homo sapiens 122-127 11099496-3 2001 Agarose bead-coated phosphorylated ITIM peptides (pITIMs) bind in vitro the single-SH2 inositol 5-phosphatases (SHIP1 and SHIP2) and the two-SH2 protein tyrosine phosphatases (SHP-1 and SHP-2). Sepharose 0-7 protein tyrosine phosphatase non-receptor type 6 Homo sapiens 176-181 11099496-3 2001 Agarose bead-coated phosphorylated ITIM peptides (pITIMs) bind in vitro the single-SH2 inositol 5-phosphatases (SHIP1 and SHIP2) and the two-SH2 protein tyrosine phosphatases (SHP-1 and SHP-2). Sepharose 0-7 protein tyrosine phosphatase non-receptor type 11 Homo sapiens 186-191 12578642-0 2001 [c-myc Antisense Oligodeoxynucleotides Induce Apoptosis of Human Myeloid Leukemia Cell Line HL-60] In order to study the effects of c-myc antisense phosphorothioate oligodeoxynucleotide in inducing apoptosis of HL-60 cells, the expression of c-myc mRNA was determined by RT-PCR, the morphologic signs of apoptotic cells were observed by transmission electron microscopy, the proportion of apoptotic cells was detected by flow cytometry, and the DNA fragments were analysed by agarose gel electrophoresis. Sepharose 476-483 MYC proto-oncogene, bHLH transcription factor Homo sapiens 1-6 11253165-3 2001 The agarose gel electrophoresis demonstrated that AVP attenuated a ladder formation stimulated by the serum deprivation. Sepharose 4-11 arginine vasopressin Rattus norvegicus 50-53 11231159-2 2001 In this system, Sepharose beads coated with Ran induce the formation of functional NEs in the absence of chromatin [1]. Sepharose 16-25 RAN, member RAS oncogene family Homo sapiens 44-47 11327827-6 2001 The zymogen, with a molecular mass of 28 kDa, was readily activated by agarose-immobilized trypsin to generate prostin, a serine protease, which cleaves the chromogenic substrate (N-benzoyl-L-Ile-L-Glu-L-Gly-L-Arg-p-nitroaniline hydrochloride) (S-2222). Sepharose 71-78 coagulation factor II, thrombin Homo sapiens 122-137 11166769-5 2001 Versican was almost completely extracted with 4 M guanidine hydrochloride in the presence of Triton X-100, isolated by chromatography on DEAE-Sephacel and characterized using treatment with specific chondro-/dermato-lyases and agarose gel electrophoresis. Sepharose 227-234 versican Homo sapiens 0-8 11157483-5 2001 Heavy and light chains of FVIII were detected in plasma-derived immune complexes extracted by using protein G Sepharose. Sepharose 110-119 coagulation factor VIII Homo sapiens 26-31 11255129-1 2001 Homogeneous casein kinase type 2 (CK2) was obtained from oocytes of Rana temporaria and cells of Drosophila melanogaster by chromatography on heparin-Sepharose, phosphocellulose, and Mono Q columns using a Pharmacia FPLC system. Sepharose 150-159 casein kinase IIalpha Drosophila melanogaster 34-37 11152530-5 2001 As cells expressed bcl-2 at higher levels, they became more capable of growing in soft agarose and became resistant to apoptosis. Sepharose 87-94 BCL2 apoptosis regulator Homo sapiens 19-24 11239513-3 2001 DESIGN AND METHODS: IgA1 was purified from sera of 6 DM patients and 4 healthy matched controls by using highly acetylated-Sepharose 6B, anti-IgA-agarose, and jacalin-agarose columns, and further separated into jacalin low-affinity, medium, and high-affinity fractions. Sepharose 146-153 immunoglobulin heavy constant alpha 1 Homo sapiens 20-24 11239513-3 2001 DESIGN AND METHODS: IgA1 was purified from sera of 6 DM patients and 4 healthy matched controls by using highly acetylated-Sepharose 6B, anti-IgA-agarose, and jacalin-agarose columns, and further separated into jacalin low-affinity, medium, and high-affinity fractions. Sepharose 167-174 immunoglobulin heavy constant alpha 1 Homo sapiens 20-24 11168395-3 2001 The basic-region and leucine zipper (bZip) domain of c-fos was expressed as a fusion protein with glutathione S-transferase, and it was bound to glutathione-agarose. Sepharose 157-164 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 53-58 11168396-2 2001 Significant differences were observed; p53CD produced a relatively small and continuous retardation of scDNA, in contrast to the ladder of distinct bands formed by p53 in agarose gels. Sepharose 171-178 tumor protein p53 Homo sapiens 164-167 11162381-5 2001 When bacterial cell lysate containing overexpressed GST-Csk was applied to a column of Zn2+-iminodiacetic acid immobilized to agarose, Csk was specifically retained by the column. Sepharose 126-133 C-terminal Src kinase Homo sapiens 56-59 11162381-5 2001 When bacterial cell lysate containing overexpressed GST-Csk was applied to a column of Zn2+-iminodiacetic acid immobilized to agarose, Csk was specifically retained by the column. Sepharose 126-133 C-terminal Src kinase Homo sapiens 135-138 11162381-9 2001 The 1 M imidazole-eluted fraction contained pure Csk that had a specific activity similar to the enzyme purified by a glutathione-agarose affinity column. Sepharose 130-137 C-terminal Src kinase Homo sapiens 49-52 11162401-10 2001 The four scFv antibody fragments bound specifically to ED-B-modified Sepharose and binding was further confirmed by immunofluorescence on cell cultures using ED-B-positive human Caco-2 tumor cells. Sepharose 69-78 immunglobulin heavy chain variable region Homo sapiens 9-13 11162401-10 2001 The four scFv antibody fragments bound specifically to ED-B-modified Sepharose and binding was further confirmed by immunofluorescence on cell cultures using ED-B-positive human Caco-2 tumor cells. Sepharose 69-78 fibronectin 1 Homo sapiens 55-59 11162401-10 2001 The four scFv antibody fragments bound specifically to ED-B-modified Sepharose and binding was further confirmed by immunofluorescence on cell cultures using ED-B-positive human Caco-2 tumor cells. Sepharose 69-78 fibronectin 1 Homo sapiens 158-162 11217018-3 2001 The fraction with an average VI content of 8.5% was most efficient as a reusable displacer for IMAC of ovalbumin, lysozyme and other proteins of egg white on Cu2+-IDA-Sepharose. Sepharose 167-176 lysozyme Homo sapiens 114-122 11680876-9 2001 Already glycopeptides from asialofetuin and ovalbumin were successfully captured by galectin-agarose and Con A-agarose, respectively. Sepharose 93-100 Galectin Caenorhabditis elegans 84-92 11163598-5 2001 Immunoblotting revealed that the predominant molecular species of VEGF concentrated with heparin-sepharose beads was VEGF(188). Sepharose 97-106 vascular endothelial growth factor A Rattus norvegicus 66-70 11206779-2 2001 Lac repressor elutes at lower heparin concentrations from a lower affinity lactose operatorl (Op1)-Sepharose column than from a higher affinity column containing the same sequence with a T18:A18 tail (Op1T18). Sepharose 99-108 bone morphogenetic protein 7 Homo sapiens 94-97 11206779-3 2001 A bi-column method was developed in which lac repressor fusion protein is eluted from the Op1-Sepharose with a low heparin concentration and trapped on a Op1T18-Sepharose column because of its higher affinity for the lac repressor protein. Sepharose 94-103 bone morphogenetic protein 7 Homo sapiens 90-93 11163598-5 2001 Immunoblotting revealed that the predominant molecular species of VEGF concentrated with heparin-sepharose beads was VEGF(188). Sepharose 97-106 vascular endothelial growth factor A Rattus norvegicus 117-121 11392817-2 2001 Our previous investigation of the total DNA from mdx mice myocardium in 0.5% agarose and 5% PAAG revealed middle sized DNA fragments about 64 kb only. Sepharose 77-84 dystrophin, muscular dystrophy Mus musculus 49-52 12233737-1 2001 The purification of human IgG3 subclass out of IgG (Immunoglobulin-G) was studied using protein A-Sepharose affinity chromatography. Sepharose 98-107 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 26-30 12064590-8 2001 Furthermore we found that ZO-1 could bind to peptides derived from the carboxyl terminal of Cx45 that had been covalently bound to an agarose resin. Sepharose 134-141 tight junction protein 1 Homo sapiens 26-30 12064590-8 2001 Furthermore we found that ZO-1 could bind to peptides derived from the carboxyl terminal of Cx45 that had been covalently bound to an agarose resin. Sepharose 134-141 gap junction protein gamma 1 Homo sapiens 92-96 11173927-9 2001 However, tyrosine phosphorylation levels in the protein components purified with p13(suc1) agarose (p13(suc1) complex) from RPTPgamma-expressing cells were different from those of the control cells. Sepharose 91-98 protein tyrosine phosphatase, receptor type, G Rattus norvegicus 124-133 11137137-6 2001 Furthermore, Lyn bound to ubiquitin-conjugated Sepharose beads in vitro and was efficiently competed by soluble ubiquitin. Sepharose 47-56 LYN proto-oncogene, Src family tyrosine kinase Rattus norvegicus 13-16 11169735-8 2001 TIMP-4 was purified by reverse phase HPLC and gelatin-sepharose affinity chromatography. Sepharose 54-63 TIMP metallopeptidase inhibitor 4 Homo sapiens 0-6 11150552-6 2001 As vitronectin and fibronectin each bind to heparin, these molecules are removed first and the heparin-Sepharose depletion occurs last in the sequence. Sepharose 103-112 vitronectin Homo sapiens 3-14 11150552-6 2001 As vitronectin and fibronectin each bind to heparin, these molecules are removed first and the heparin-Sepharose depletion occurs last in the sequence. Sepharose 103-112 fibronectin 1 Homo sapiens 19-30 11269509-6 2001 Then, the size of the frataxin repeat was determined by polymerase chain reaction (PCR) and agarose gel electrophoresis. Sepharose 92-99 frataxin Homo sapiens 22-30 11160803-4 2001 The 58 kDa GroEL protein was expressed in Escherichia coli in fusion with glutathione S:-transferase and the fusion protein was purified from IPTG-induced bacterial lysates by affinity chromatography on glutathione-Sepharose. Sepharose 215-224 GroEL Escherichia coli 11-16 11274797-3 2001 In this study, a linear concentration gradient of nerve growth factor was achieved across a 5-mm agarose membrane that separated a nerve growth factor source compartment from a sink compartment. Sepharose 97-104 nerve growth factor Rattus norvegicus 50-69 11680695-5 2001 To evaluate the effect of ChM-I on ectopic bone formation, guanidine extracts of demineralized bone matrix were mixed with the ChM-I-bound heparin-Sepharose beads and were implanted onto the fasciae of back muscle of 6-week old nude mice. Sepharose 147-156 chondromodulin Mus musculus 127-132 11177613-5 2000 IgH PCR with agarose gel detection was performed between framework regions FR3 and FR1, both in combination with 5 different primers in FR4. Sepharose 13-20 immunoglobulin heavy locus Homo sapiens 0-3 11780582-5 2001 MUC 1 antigen was isolated from human gastric juice by the gel filtration on Sepharose 4B column and affinity chromatography with HMFG-1 antibody coupled to CNBr activated Sepharose 4B. Sepharose 77-86 mucin 1, cell surface associated Homo sapiens 0-5 11780582-5 2001 MUC 1 antigen was isolated from human gastric juice by the gel filtration on Sepharose 4B column and affinity chromatography with HMFG-1 antibody coupled to CNBr activated Sepharose 4B. Sepharose 172-181 mucin 1, cell surface associated Homo sapiens 0-5 11156405-1 2000 A novel gene, designated UROC28, was identified by an agarose gel-based differential display technique, and it was found to be up-regulated in prostate, breast, and bladder cancer. Sepharose 54-61 prostate and breast cancer overexpressed 1 Homo sapiens 25-31 11076803-3 2000 Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. Sepharose 0-9 C-X-C motif chemokine ligand 8 Homo sapiens 22-26 11076803-3 2000 Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. Sepharose 0-9 C-X-C motif chemokine ligand 8 Homo sapiens 62-66 11076803-3 2000 Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. Sepharose 0-9 C-X-C motif chemokine ligand 8 Homo sapiens 62-66 11076803-3 2000 Sepharose-immobilized IL-8 stimulated a sevenfold increase in IL-8 production within 2 h. IL-8 induced the expression of its own message, and IL-8 biosynthesis was inhibited by cycloheximide and actinomycin D, indicating de novo RNA and protein synthesis. Sepharose 0-9 C-X-C motif chemokine ligand 8 Homo sapiens 62-66 11131610-3 2000 PROCEDURES: The feline hepatic insulin receptor was purified from Triton X-100 solubilized plasma membranes by the use of several chromatography matrices, including affinity chromatography on an insulin-Sepharose matrix. Sepharose 203-212 insulin receptor Felis catus 31-47 11131610-3 2000 PROCEDURES: The feline hepatic insulin receptor was purified from Triton X-100 solubilized plasma membranes by the use of several chromatography matrices, including affinity chromatography on an insulin-Sepharose matrix. Sepharose 203-212 insulin Felis catus 31-38 11090059-4 2000 Binding studies using the 165 amino acid form of VEGF immobilized on Sepharose beads and soluble iodine 125 ((125)I)-labeled fibrinogen demonstrated saturable and specific binding. Sepharose 69-78 vascular endothelial growth factor A Homo sapiens 49-53 11082199-10 2000 In summary, the cytochalasin B-binding state of GLUT1 seemed to be affected by (a) biotinylation of the cell surface, (b) removal of the cytoskeleton at high pH and low ionic strength, (c) interaction between the dextran-grafted agarose gel matrix and the membrane vesicles, and (d) reconstitution to form proteoliposomes. Sepharose 229-236 solute carrier family 2 member 1 Homo sapiens 48-53 11108742-1 2000 Pre-beta1-HDL, a putative discoid-shaped high density lipoprotein (HDL) of approximately 67-kDa mass that migrates with pre-beta mobility in agarose gel electrophoresis, contains apolipoprotein A-I (apoA-I), phospholipids, and unesterified cholesterol. Sepharose 141-148 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 4-9 12092657-4 2001 A heterozygous deleted mutation was detected in K-RAS oncogene (exon 2) in agarose gel electrophoresis in one patient and point or substitution mutations are detected using single strand conformational polymorphism (SSCP) in other different patients with bladder cancer (4/14). Sepharose 75-82 KRAS proto-oncogene, GTPase Homo sapiens 48-53 11102533-5 2000 GST-tagged Bet3p or Bet5p, two of the TRAPP subunits, were expressed in yeast cells and were precipitated by glutathione-agarose (GA) beads. Sepharose 121-128 TRAPP complex core subunit BET3 Saccharomyces cerevisiae S288C 11-16 11102533-5 2000 GST-tagged Bet3p or Bet5p, two of the TRAPP subunits, were expressed in yeast cells and were precipitated by glutathione-agarose (GA) beads. Sepharose 121-128 TRAPP subunit BET5 Saccharomyces cerevisiae S288C 20-25 11399324-10 2000 Furthermore, the percentage of direct binding of 125I-labeled StAb or monomeric scFv on CEA-Sepharose beads and on CEA-expressing cells showed a dramatic increase for the tetramerized scFv (>80%), as compared with the monomeric scFv (<20%). Sepharose 92-101 immunglobulin heavy chain variable region Homo sapiens 80-84 11399324-10 2000 Furthermore, the percentage of direct binding of 125I-labeled StAb or monomeric scFv on CEA-Sepharose beads and on CEA-expressing cells showed a dramatic increase for the tetramerized scFv (>80%), as compared with the monomeric scFv (<20%). Sepharose 92-101 CEA cell adhesion molecule 3 Homo sapiens 88-91 11399324-10 2000 Furthermore, the percentage of direct binding of 125I-labeled StAb or monomeric scFv on CEA-Sepharose beads and on CEA-expressing cells showed a dramatic increase for the tetramerized scFv (>80%), as compared with the monomeric scFv (<20%). Sepharose 92-101 immunglobulin heavy chain variable region Homo sapiens 184-188 11399324-10 2000 Furthermore, the percentage of direct binding of 125I-labeled StAb or monomeric scFv on CEA-Sepharose beads and on CEA-expressing cells showed a dramatic increase for the tetramerized scFv (>80%), as compared with the monomeric scFv (<20%). Sepharose 92-101 immunglobulin heavy chain variable region Homo sapiens 184-188 12903404-2 2000 METHODS: The experiment was carried out under the low water activity condition, using tosylate chloride activating side-chain hydroxyl group of Sepharose CL-4B agarose to form a high active group which could react with the free amino-group of ACE to link the enzyme with agarose. Sepharose 160-167 angiotensin I converting enzyme Homo sapiens 243-246 11087679-5 2000 A polyhistidine-tagged axin peptide (residues 419-672) was produced in Escherichia coli and either immobilized on Ni-NTA agarose beads or purified and immobilized on CNBr-activated Sepharose 4B. Sepharose 121-128 axin 1 Rattus norvegicus 23-27 11087679-5 2000 A polyhistidine-tagged axin peptide (residues 419-672) was produced in Escherichia coli and either immobilized on Ni-NTA agarose beads or purified and immobilized on CNBr-activated Sepharose 4B. Sepharose 181-190 axin 1 Rattus norvegicus 23-27 11135699-2 2000 METHODS: The ARL-1 cDNA was cloned into procaryotic expression vector PQE-30 and the recombinant ARL-1 expressed in E.coli M(15); then Ni(+)-NTA agarose columns were used to purify the ARL-1 protein. Sepharose 145-152 aldo-keto reductase family 1 member B10 Homo sapiens 13-18 12903404-2 2000 METHODS: The experiment was carried out under the low water activity condition, using tosylate chloride activating side-chain hydroxyl group of Sepharose CL-4B agarose to form a high active group which could react with the free amino-group of ACE to link the enzyme with agarose. Sepharose 271-278 angiotensin I converting enzyme Homo sapiens 243-246 10903313-1 2000 The two cytosolic members of the highly conserved 70-kDa stress protein family, Ssa1p and Ssa2p, were specifically retained by the prepro-NH(2) extension of the vacuolar aminopeptidase I precursor (pAPI) conjugated to agarose (Sulfolink). Sepharose 218-225 Hsp70 family ATPase SSA1 Saccharomyces cerevisiae S288C 80-85 10903313-1 2000 The two cytosolic members of the highly conserved 70-kDa stress protein family, Ssa1p and Ssa2p, were specifically retained by the prepro-NH(2) extension of the vacuolar aminopeptidase I precursor (pAPI) conjugated to agarose (Sulfolink). Sepharose 218-225 Hsp70 family chaperone SSA2 Saccharomyces cerevisiae S288C 90-95 11097156-8 2000 RESULTS: Clear bands expressing cDNA of iNOS mRNA were identified in agarose gels in all cultured cells in both groups. Sepharose 69-76 nitric oxide synthase 2 Homo sapiens 40-44 11137456-1 2000 A prolyl endopeptidase (PE) was purified 83 times from human urine by DEAE-cellulose and Sepharose Mercurial chromatographies. Sepharose 89-98 prolyl endopeptidase Homo sapiens 2-22 11137456-1 2000 A prolyl endopeptidase (PE) was purified 83 times from human urine by DEAE-cellulose and Sepharose Mercurial chromatographies. Sepharose 89-98 prolyl endopeptidase Homo sapiens 24-26 11069705-8 2000 The higher molecular weight immunoreactive protein was also present in soluble extracts and bound to calmodulin-agarose in the presence of calcium, demonstrating that native LeCBDGK is a calmodulin-binding protein. Sepharose 112-119 calcium-binding protein CP1 Solanum lycopersicum 101-111 11041873-8 2000 Affinity chromatography analysis showed that mMCP-6 bound strongly to heparin-Sepharose at pH 6.0 but not at neutral pH. Sepharose 78-87 tryptase beta 2 Mus musculus 45-51 11029041-4 2000 Isolation and analysis of gelatinases from conditioned media by gelatin-agarose affinity chromatography and gelatinolytic assays demonstrated that TSP2-null fibroblasts produce a 2-fold increase in gelatinase A (MMP2) compared with wild-type cells. Sepharose 72-79 thrombospondin 2 Mus musculus 147-151 10993209-1 2000 The inhibitory effects of natural and synthetic inhibitors on the intestinal membrane-bound hydrolase, alpha-glucosidase (AGH), were evaluated by using an immobilized cyanogen bromide-activated Sepharose 4B support. Sepharose 194-203 sucrase-isomaltase Homo sapiens 103-120 10988247-5 2000 Human antibodies affinity-purified on a synthetic Gal(beta1-4)Gal(beta1-4)Glc-Sepharose column recognized the newly characterized Gal(beta1-4)Gal-terminated pentaglycosylceramide, and, in addition, longer chain glycolipids. Sepharose 78-87 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 54-61 10988247-5 2000 Human antibodies affinity-purified on a synthetic Gal(beta1-4)Gal(beta1-4)Glc-Sepharose column recognized the newly characterized Gal(beta1-4)Gal-terminated pentaglycosylceramide, and, in addition, longer chain glycolipids. Sepharose 78-87 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 66-73 10988247-5 2000 Human antibodies affinity-purified on a synthetic Gal(beta1-4)Gal(beta1-4)Glc-Sepharose column recognized the newly characterized Gal(beta1-4)Gal-terminated pentaglycosylceramide, and, in addition, longer chain glycolipids. Sepharose 78-87 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 66-73 10945828-5 2000 Moesin phosphorylated in mast cells treated with cromolyn shifted from the soluble to the precipitable fraction and associated with Sepharose-linked beta-actin. Sepharose 132-141 moesin Rattus norvegicus 0-6 10945828-5 2000 Moesin phosphorylated in mast cells treated with cromolyn shifted from the soluble to the precipitable fraction and associated with Sepharose-linked beta-actin. Sepharose 132-141 actin, beta Rattus norvegicus 149-159 10945828-6 2000 Recombinant moesin also associated with Sepharose-linked beta-actin, and so did purified RBL moesin, but only if the latter was first denatured. Sepharose 40-49 moesin Rattus norvegicus 12-18 10945828-6 2000 Recombinant moesin also associated with Sepharose-linked beta-actin, and so did purified RBL moesin, but only if the latter was first denatured. Sepharose 40-49 actin, beta Rattus norvegicus 57-67 10998179-5 2000 Native agarose gel electrophoresis confirmed that FliH and FliI form a complex. Sepharose 7-14 FLII actin remodeling protein Homo sapiens 59-63 11877016-3 2000 Human vWF was purified from blood cryoprecipitate by glycine and NaCl precipitation and subsequent separation on sepharose 4B column. Sepharose 113-125 von Willebrand factor Homo sapiens 6-9 10956019-1 2000 A novel thrombin-like enzyme (named contortrixobin) has been purified to homogeneity from the venom of Agkistrodon contortrix contortrix by affinity chromatography on arginine-Sepharose, anionic exchange chromatography, and HPLC. Sepharose 176-185 coagulation factor II, thrombin Homo sapiens 8-16 11009029-4 2000 LEC-6 was coupled to N-hydroxysuccinimide-activated Sepharose 4 Fast Flow (100 microm diameter), and packed into a miniature column (e.g., 10 x 4.0 mm, 0.126 ml). Sepharose 52-61 Galectin Caenorhabditis elegans 0-5 10925288-5 2000 After triggering the C1 by IgG-Sepharose, both C1s subunits are cleaved by the single proteolytically active C1r subunit in the C1s-C1rQI-C1r-C1s tetramer. Sepharose 31-40 complement C1s Homo sapiens 47-50 10925288-5 2000 After triggering the C1 by IgG-Sepharose, both C1s subunits are cleaved by the single proteolytically active C1r subunit in the C1s-C1rQI-C1r-C1s tetramer. Sepharose 31-40 complement C1r Homo sapiens 109-112 10925288-5 2000 After triggering the C1 by IgG-Sepharose, both C1s subunits are cleaved by the single proteolytically active C1r subunit in the C1s-C1rQI-C1r-C1s tetramer. Sepharose 31-40 complement C1s Homo sapiens 128-131 10925288-5 2000 After triggering the C1 by IgG-Sepharose, both C1s subunits are cleaved by the single proteolytically active C1r subunit in the C1s-C1rQI-C1r-C1s tetramer. Sepharose 31-40 complement C1r Homo sapiens 132-137 10925288-5 2000 After triggering the C1 by IgG-Sepharose, both C1s subunits are cleaved by the single proteolytically active C1r subunit in the C1s-C1rQI-C1r-C1s tetramer. Sepharose 31-40 complement C1r Homo sapiens 132-135 10925288-5 2000 After triggering the C1 by IgG-Sepharose, both C1s subunits are cleaved by the single proteolytically active C1r subunit in the C1s-C1rQI-C1r-C1s tetramer. Sepharose 31-40 complement C1s Homo sapiens 128-131 10945626-3 2000 When immobilized on Sepharose beads, these antibodies allow a high-yield, one-step purification of native gp96 molecules from both mouse and human tumor cell lysates. Sepharose 20-29 heat shock protein 90, beta (Grp94), member 1 Mus musculus 106-110 10923043-2 2000 A new method, which could detect base pair mismatches with Rnase cleavage on agarose gel electrophoresis, identified 5 and 4 mutations of the MPZ and Cx32 genes, respectively, including one novel mutation (Ser128Ter) of Cx32. Sepharose 77-84 myelin protein zero Homo sapiens 142-145 10923043-2 2000 A new method, which could detect base pair mismatches with Rnase cleavage on agarose gel electrophoresis, identified 5 and 4 mutations of the MPZ and Cx32 genes, respectively, including one novel mutation (Ser128Ter) of Cx32. Sepharose 77-84 gap junction protein beta 1 Homo sapiens 150-154 10923043-2 2000 A new method, which could detect base pair mismatches with Rnase cleavage on agarose gel electrophoresis, identified 5 and 4 mutations of the MPZ and Cx32 genes, respectively, including one novel mutation (Ser128Ter) of Cx32. Sepharose 77-84 gap junction protein beta 1 Homo sapiens 220-224 11082041-4 2000 HA coupled to Sepharose is able to precipitate SorLA specifically proving that SorLA binds HA. Sepharose 14-23 sortilin related receptor 1 Homo sapiens 47-52 11082041-4 2000 HA coupled to Sepharose is able to precipitate SorLA specifically proving that SorLA binds HA. Sepharose 14-23 sortilin related receptor 1 Homo sapiens 79-84 11392781-1 2000 Myometrium cell plasma membrane Ca2+, Mg(2+)-ATPase purified by an affinity chromatography on calmodulin-sepharose 4B is calmodulin-dependent enzyme. Sepharose 105-114 calmodulin 1 Homo sapiens 94-104 11392781-1 2000 Myometrium cell plasma membrane Ca2+, Mg(2+)-ATPase purified by an affinity chromatography on calmodulin-sepharose 4B is calmodulin-dependent enzyme. Sepharose 105-114 calmodulin 1 Homo sapiens 121-131 10903319-1 2000 A new beta1,4-N-acetylglucosaminyltransferase (GnT) responsible for the formation of branched N-linked complex-type sugar chains has been purified 64,000-fold in 16% yield from a homogenate of hen oviduct by column chromatography procedures using Q-Sepharose FF, Ni(2+)-chelating Sepharose FF, and UDP-hexanolamine-agarose. Sepharose 249-258 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 6-11 10903319-1 2000 A new beta1,4-N-acetylglucosaminyltransferase (GnT) responsible for the formation of branched N-linked complex-type sugar chains has been purified 64,000-fold in 16% yield from a homogenate of hen oviduct by column chromatography procedures using Q-Sepharose FF, Ni(2+)-chelating Sepharose FF, and UDP-hexanolamine-agarose. Sepharose 315-322 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 6-11 11035682-7 2000 ACE genotyping was performed on DNA samples from patients using the polymerase chain reaction followed by agarose gel electrophoresis. Sepharose 106-113 angiotensin I converting enzyme Homo sapiens 0-3 11013299-3 2000 It has been shown, however, that only a fraction of plasma Lp[a] (Lp[a]-Lys(+)) binds to lysine-Sepharose in vitro. Sepharose 96-105 lipoprotein(a) Homo sapiens 59-63 11013299-3 2000 It has been shown, however, that only a fraction of plasma Lp[a] (Lp[a]-Lys(+)) binds to lysine-Sepharose in vitro. Sepharose 96-105 lipoprotein(a) Homo sapiens 66-70 11013299-10 2000 These interactions appear to mask the lysine-binding site in apo[a] kringle IV type 10, which mediates the interaction of Lp[a] with lysine-Sepharose. Sepharose 140-149 lipoprotein(a) Homo sapiens 122-126 11073083-3 2000 The recombinant IL-2 (rIL-2) was purified by a batch method using Glutathione Sepharose 4B and factor Xa digestion and used for preparation of antisera in mice. Sepharose 78-87 interleukin 2 Mus musculus 16-20 11073083-3 2000 The recombinant IL-2 (rIL-2) was purified by a batch method using Glutathione Sepharose 4B and factor Xa digestion and used for preparation of antisera in mice. Sepharose 78-87 interleukin 2 Rattus norvegicus 22-27 11006108-1 2000 Using biotinylated adrenodoxin and avidin-Sepharose 4B, dissociation constants for the complex between adrenodoxin reductase and adrenodoxin in the oxidized and reduced states were determined as 50 +/- 11 and 296 +/- 44 nM, respectively. Sepharose 42-51 ferredoxin reductase Bos taurus 103-124 10961890-3 2000 It was demonstrated by affinity chromatography that hIL-10 binds strongly to heparin-agarose at physiological pH. Sepharose 85-92 interleukin 10 Homo sapiens 52-58 10993483-2 2000 This assertion is based on the observations (1) TNF-alpha increased the number of cells with hypo-diploid DNA in a time dependent manner as revealed by flow cytometry, and (2) TNF-alpha induced DNA fragmentation as resolved by agarose gel electrophoresis. Sepharose 227-234 tumor necrosis factor Mus musculus 176-185 11083457-3 2000 In this study, human platelet GGT was solubilized with Triton X-100 and purified by lectin affinity chromatography on Con A Sepharose 4B to determine its electrophoretic properties. Sepharose 124-136 gamma-glutamyltransferase 1 Homo sapiens 30-33 10821830-6 2000 In reducing agarose gels, EMILIN also migrated as a trimer, whereas under non-reducing conditions it formed polymers of many millions of daltons. Sepharose 12-19 elastin microfibril interfacer 1 Homo sapiens 26-32 10931206-9 2000 Recombinant PP2A subunits were able to form a complex as demonstrated both by activity assays in the presence of protamine and by chromatography on protamine-agarose. Sepharose 158-165 protein phosphatase 2 phosphatase activator Homo sapiens 12-16 11819639-8 2000 Fourty-eight h and 72 h of p27(KIP1) expression showed a characteristic DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak may induce cell cycle arrest in G(1) phase through upregulating expression of p27(KIP1) and subsequently lead to apoptosis in HCC-9204 cells. Sepharose 86-93 interferon alpha inducible protein 27 Homo sapiens 27-30 11819639-8 2000 Fourty-eight h and 72 h of p27(KIP1) expression showed a characteristic DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak may induce cell cycle arrest in G(1) phase through upregulating expression of p27(KIP1) and subsequently lead to apoptosis in HCC-9204 cells. Sepharose 86-93 cyclin dependent kinase inhibitor 1B Homo sapiens 31-35 11819639-8 2000 Fourty-eight h and 72 h of p27(KIP1) expression showed a characteristic DNA ladder on agarose gel electrophoresis.CONCLUSION:Bak may induce cell cycle arrest in G(1) phase through upregulating expression of p27(KIP1) and subsequently lead to apoptosis in HCC-9204 cells. Sepharose 86-93 BCL2 antagonist/killer 1 Homo sapiens 125-128 10880356-2 2000 Here, we show that GSNO-Sepharose mimicks site-specific S-glutathionylation of the transcription factors c-Jun and p50 by free GSNO in vitro. Sepharose 24-33 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 105-110 10880356-2 2000 Here, we show that GSNO-Sepharose mimicks site-specific S-glutathionylation of the transcription factors c-Jun and p50 by free GSNO in vitro. Sepharose 24-33 nuclear factor kappa B subunit 1 Homo sapiens 115-118 10880356-4 2000 Furthermore, we show that c-Jun, p50, glycogen phosphorylase b, glyceraldehyde-3-phosphate dehydrogenase, creatine kinase, glutaredoxin and caspase-3 can be precipitated from a mixture of purified thiol-containing proteins by the formation of a mixed-disulphide bond with GSNO-Sepharose. Sepharose 277-286 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 26-31 10880356-4 2000 Furthermore, we show that c-Jun, p50, glycogen phosphorylase b, glyceraldehyde-3-phosphate dehydrogenase, creatine kinase, glutaredoxin and caspase-3 can be precipitated from a mixture of purified thiol-containing proteins by the formation of a mixed-disulphide bond with GSNO-Sepharose. Sepharose 277-286 caspase 3 Homo sapiens 140-149 10889507-4 2000 Affinity chromatography on m7GTP-Sepharose shows that M-FAG retains the ability of eIF4GI to associate with both the mRNA cap-binding protein eIF4E and initiation factor eIF4A and that the ribosome-bound form of M-FAG is also present as a complex with eIF4E and eIF4A. Sepharose 33-42 eukaryotic translation initiation factor 4 gamma 1 Homo sapiens 83-89 10987507-1 2000 The immunohistochemical analysis of the HNK-1 epitope presence in the liver and upper digestive tract nerves was carried out in 12- to 18-day-old rat embryos embedded in acrylamide-agarose and observed with laser scanning confocal microscopy. Sepharose 181-188 beta-1,3-glucuronyltransferase 1 Rattus norvegicus 40-45 10889253-6 2000 This differential binding capacity was confirmed by an affinity chromatography of R-FNR on Fd-sepharose with stronger binding to Fd III. Sepharose 94-103 ferredoxin Zea mays 84-87 10889253-6 2000 This differential binding capacity was confirmed by an affinity chromatography of R-FNR on Fd-sepharose with stronger binding to Fd III. Sepharose 94-103 ferredoxin-3, chloroplastic Zea mays 129-135 10856318-6 2000 Rat bronchoalveolar lavage (BAL) fluid was enriched for surfactant protein D (SP-D) using maltose agarose affinity chromatography. Sepharose 98-105 surfactant protein D Rattus norvegicus 56-76 10856318-6 2000 Rat bronchoalveolar lavage (BAL) fluid was enriched for surfactant protein D (SP-D) using maltose agarose affinity chromatography. Sepharose 98-105 surfactant protein D Rattus norvegicus 78-82 10749870-7 2000 Affinity coelectrophoresis analysis of a subset of samples demonstrated that increased interaction of proteoglycan with HCII in agarose gels paralleled increased activity in thrombin-HCII inhibition assays. Sepharose 128-135 serpin family D member 1 Homo sapiens 120-124 10849447-11 2000 Recombinant ADAM 12-S partially purified from conditioned medium on a heparin-Sepharose column also proteolyzed IGFBP-3. Sepharose 78-87 ADAM metallopeptidase domain 12 Homo sapiens 12-19 10849447-11 2000 Recombinant ADAM 12-S partially purified from conditioned medium on a heparin-Sepharose column also proteolyzed IGFBP-3. Sepharose 78-87 insulin like growth factor binding protein 3 Homo sapiens 112-119 10836986-2 2000 Partial purification of ATP diphosphohydrolase (ATPDase) was achieved by successive chromatography on concanavalin A-Sepharose, Q-Sepharose Fast Flow, and 5"-AMP-Sepharose 4B. Sepharose 117-126 ectonucleoside triphosphate diphosphohydrolase 1 Sus scrofa 24-46 10836986-2 2000 Partial purification of ATP diphosphohydrolase (ATPDase) was achieved by successive chromatography on concanavalin A-Sepharose, Q-Sepharose Fast Flow, and 5"-AMP-Sepharose 4B. Sepharose 117-126 ectonucleoside triphosphate diphosphohydrolase 1 Sus scrofa 48-55 10910095-3 2000 Activation of M+ Akt:ER* in Rat1 cells elicited alterations in cell morphology and promoted anchorage-independent growth in agarose with high efficiency. Sepharose 124-131 AKT serine/threonine kinase 1 Rattus norvegicus 17-20 10848900-8 2000 Partial purification of a putative sheep tryptase from lung and gut extracts was achieved using heparin-Sepharose affinity chromatography. Sepharose 104-113 tryptase beta-2 Ovis aries 41-49 10885198-2 2000 Plasma samples treated with neuraminidase and carboxypeptidase-B were subjected to high-voltage agarose gel electrophoresis followed by immunofixation. Sepharose 96-103 neuraminidase 1 Homo sapiens 28-41 10885198-2 2000 Plasma samples treated with neuraminidase and carboxypeptidase-B were subjected to high-voltage agarose gel electrophoresis followed by immunofixation. Sepharose 96-103 carboxypeptidase B1 Homo sapiens 46-64 10833390-0 2000 The preparation of apo-Cu,Zn superoxide dismutase by ion-exchange chromatography on iminodiacetic acid-sepharose. Sepharose 103-112 superoxide dismutase [Cu-Zn] Bos taurus 23-49 10851070-6 2000 Furthermore, when T/t-common was transiently cotransfected with the activated genomic neu into NIH3T3 cells, the transforming activity of the latter was suppressed by T/t-common in soft-agarose microcolony formation assays. Sepharose 186-193 erb-b2 receptor tyrosine kinase 2 Mus musculus 86-89 10839120-7 2000 Thus, the allyl group of the partially benzylated alpha-D-Galp-(1-->3)-beta-D-Galp-OAll was engineered, via the hydroxy-, the tosyloxy- and the azidopropyl intermediates, into an aminopropyl group amenable to binding to N-hydroxysuccinimide-activated agarose gel matrices in order to obtain specific immunoabsorption columns. Sepharose 254-261 galanin like peptide Homo sapiens 58-62 10839120-7 2000 Thus, the allyl group of the partially benzylated alpha-D-Galp-(1-->3)-beta-D-Galp-OAll was engineered, via the hydroxy-, the tosyloxy- and the azidopropyl intermediates, into an aminopropyl group amenable to binding to N-hydroxysuccinimide-activated agarose gel matrices in order to obtain specific immunoabsorption columns. Sepharose 254-261 galanin like peptide Homo sapiens 81-85 10817252-5 2000 Serum Lp(a) levels were measured by enzyme-linked immunosorbent assay and apo(a) isoforms determined by a high-resolution sodium dodecyl sulphate/agarose gel electrophoresis with immunoblotting in two groups of Kuwaiti subjects: healthy controls (n = 140) and subjects with CHD (n = 140). Sepharose 146-153 lipoprotein(a) Homo sapiens 74-80 10851030-3 2000 The human LBP was expressed by E. coli cells, and it was purified using Ni-NTA-Sepharose chromatography. Sepharose 79-88 galectin 3 Homo sapiens 10-13 10772945-5 2000 In cells preincubated with IGF-1 before the addition of SOR, we detected an increase in the number of viable cells, a decrease in the generation of DNA fragments on agarose gel electrophoresis and in the percentage of positive TUNEL cells, and a reduction on PARP levels. Sepharose 165-172 insulin like growth factor 1 Homo sapiens 27-32 10806244-1 2000 High-isoelectric-point (pI) alpha-glucosidase was purified 7, 300-fold from an extract of barley (Hordeum vulgare) malt by ammonium sulfate fractionation, ion-exchange, and butyl-Sepharose chromatography. Sepharose 179-188 Agl1 Hordeum vulgare 28-45 10753822-7 2000 alpha-Actinin and moesin are preferentially adsorbed on Sepharose beads bearing a recombinant CD43 intracellular domain. Sepharose 56-65 actinin alpha 1 Homo sapiens 0-13 10753822-7 2000 alpha-Actinin and moesin are preferentially adsorbed on Sepharose beads bearing a recombinant CD43 intracellular domain. Sepharose 56-65 moesin Homo sapiens 18-24 10753822-7 2000 alpha-Actinin and moesin are preferentially adsorbed on Sepharose beads bearing a recombinant CD43 intracellular domain. Sepharose 56-65 sialophorin Homo sapiens 94-98 10725457-5 2000 We report here that hsp40, hsp60, hsc70, hsp70, hsp84, hsp86, and gp96 (grp94) but not BiP (grp78) and calreticulin can be separated from a single tumor sample in one step using heparin-agarose chromatography. Sepharose 186-193 DnaJ heat shock protein family (Hsp40) member B1 pseudogene 1 Homo sapiens 20-25 10749676-9 2000 Affinity chromatography with a polyhistidine-containing peptide immobilized on agarose revealed that TSP1 in platelet releasates is the major polypeptide retained on the six-histidine-peptide column. Sepharose 79-86 thrombospondin 1 Homo sapiens 101-105 10713316-1 2000 Fibrinogen was purified by protamine-agarose chromatography from plasma from three patients after their submission to hospital due to acute myocardial infarction. Sepharose 37-44 fibrinogen beta chain Homo sapiens 0-10 10725457-5 2000 We report here that hsp40, hsp60, hsc70, hsp70, hsp84, hsp86, and gp96 (grp94) but not BiP (grp78) and calreticulin can be separated from a single tumor sample in one step using heparin-agarose chromatography. Sepharose 186-193 heat shock protein family A (Hsp70) member 8 Homo sapiens 34-39 10725457-5 2000 We report here that hsp40, hsp60, hsc70, hsp70, hsp84, hsp86, and gp96 (grp94) but not BiP (grp78) and calreticulin can be separated from a single tumor sample in one step using heparin-agarose chromatography. Sepharose 186-193 heat shock protein family A (Hsp70) member 4 Homo sapiens 41-46 10725457-5 2000 We report here that hsp40, hsp60, hsc70, hsp70, hsp84, hsp86, and gp96 (grp94) but not BiP (grp78) and calreticulin can be separated from a single tumor sample in one step using heparin-agarose chromatography. Sepharose 186-193 heat shock protein 90 alpha family class B member 1 Homo sapiens 48-53 10725457-5 2000 We report here that hsp40, hsp60, hsc70, hsp70, hsp84, hsp86, and gp96 (grp94) but not BiP (grp78) and calreticulin can be separated from a single tumor sample in one step using heparin-agarose chromatography. Sepharose 186-193 heat shock protein 90 beta family member 1 Homo sapiens 66-70 10725457-5 2000 We report here that hsp40, hsp60, hsc70, hsp70, hsp84, hsp86, and gp96 (grp94) but not BiP (grp78) and calreticulin can be separated from a single tumor sample in one step using heparin-agarose chromatography. Sepharose 186-193 heat shock protein 90 beta family member 1 Homo sapiens 72-77 10727399-4 2000 The MUC5AC reduced-mucin subunits exhibited a homogeneous charge distribution on anion-exchange chromatography, but appeared as two bands, one major and one more minor, after agarose gel electrophoresis. Sepharose 175-182 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 4-10 10810185-6 2000 A withdrawal of the two-enzyme complexes from the erythrocyte lysates using Sepharose-bound anti-GAPDH antibodies prevents the pH-dependent accumulation of the metabolites. Sepharose 76-85 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 97-102 10913513-3 2000 Isopycnic density gradient centrifugation gave good resolution of mature and precursor forms of MUC2 as assessed by agarose gel electrophoresis. Sepharose 116-123 mucin 2, oligomeric mucus/gel-forming Homo sapiens 96-100 10727399-4 2000 The MUC5AC reduced-mucin subunits exhibited a homogeneous charge distribution on anion-exchange chromatography, but appeared as two bands, one major and one more minor, after agarose gel electrophoresis. Sepharose 175-182 LOC100508689 Homo sapiens 19-24 10727399-6 2000 Agarose gel electrophoresis of unreduced MUC5AC mucins identified a major band just entering the gel with evidence of a "ladder" of faster-migrating minor bands. Sepharose 0-7 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 41-47 10727399-11 2000 In addition the MUC5AC mucin from respiratory tract secretions exhibited similar behaviour, reduced and unreduced on agarose gel electrophoresis, indicating that the mucin has a similar molecular phenotype in vivo and in vitro. Sepharose 117-124 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 16-22 10727399-11 2000 In addition the MUC5AC mucin from respiratory tract secretions exhibited similar behaviour, reduced and unreduced on agarose gel electrophoresis, indicating that the mucin has a similar molecular phenotype in vivo and in vitro. Sepharose 117-124 LOC100508689 Homo sapiens 23-28 11112095-8 2000 Fucoidan-Sepharose affinity chromatography revealed that the binding of PAI-1 with fucoidan may be responsible for the reversal of the enhancement by fucoidan-6-AH. Sepharose 9-18 serpin family E member 1 Homo sapiens 72-77 10719057-8 2000 Using anti-MIF antibody-immobilized sepharose column chromatography, we discovered for the first time that MIF was present in erythrocytes. Sepharose 36-45 macrophage migration inhibitory factor Homo sapiens 11-14 10719057-8 2000 Using anti-MIF antibody-immobilized sepharose column chromatography, we discovered for the first time that MIF was present in erythrocytes. Sepharose 36-45 macrophage migration inhibitory factor Homo sapiens 107-110 12548934-4 2000 The hGSTA1 from both E. coli and L. lactis was purified by affinity chromatography on glutathione-agarose and all showed enzymatic activity. Sepharose 98-105 glutathione S-transferase alpha 1 Homo sapiens 4-10 10752523-9 2000 Affinity precipitation of biotin-labeled HUVEC membrane proteins with protein G-Sepharose bearing PR3 resulted in specific precipitation of a membrane molecule with a molecular weight of 111 kD under nonreducing conditions and 52 and 63 kD under reducing conditions. Sepharose 80-89 proteinase 3 Homo sapiens 98-101 10713175-6 2000 Using bacterially produced ARF polypeptides and chemically synthesized peptides conjugated to Sepharose, residues 1 to 14 and 26 to 37 of mouse p19(ARF) were found to interact independently and cooperatively with Mdm2, while residues 15 to 25 were dispensable for binding. Sepharose 94-103 interleukin 23, alpha subunit p19 Mus musculus 144-147 10713175-6 2000 Using bacterially produced ARF polypeptides and chemically synthesized peptides conjugated to Sepharose, residues 1 to 14 and 26 to 37 of mouse p19(ARF) were found to interact independently and cooperatively with Mdm2, while residues 15 to 25 were dispensable for binding. Sepharose 94-103 interleukin 23, alpha subunit p19 Mus musculus 148-151 10713175-6 2000 Using bacterially produced ARF polypeptides and chemically synthesized peptides conjugated to Sepharose, residues 1 to 14 and 26 to 37 of mouse p19(ARF) were found to interact independently and cooperatively with Mdm2, while residues 15 to 25 were dispensable for binding. Sepharose 94-103 transformed mouse 3T3 cell double minute 2 Mus musculus 213-217 10807041-4 2000 When glutathione S-transferase was purified by affinity chromatography on Sepharose-linked glutathione, incubated with lead chloride or lead acetate, a concentration-dependent inhibition of the enzymatic activity was observed reaching 50% inhibition at a lead salt concentration of 6000 microg/dl. Sepharose 74-83 glutathione S-transferase kappa 1 Homo sapiens 5-30 10734072-3 2000 Co-immunoprecipitation of prostate cancer LNCaP cell extract using protein A-Sepharose coupled with anti-AR antibody indicates that the AR interacts with the general transcription factor TFIIH in a physiological condition. Sepharose 77-86 androgen receptor Homo sapiens 136-138 10706790-1 2000 Oligonucleotides bound by the CAAT enhancer binding protein (C/EBP), the lactose repressor, and Gal4 were chemically coupled to cyanogen bromide-activated Sepharose and the temperature dependence of transcription factor chromatography was characterized. Sepharose 155-164 CCAAT enhancer binding protein alpha Homo sapiens 30-59 10734072-3 2000 Co-immunoprecipitation of prostate cancer LNCaP cell extract using protein A-Sepharose coupled with anti-AR antibody indicates that the AR interacts with the general transcription factor TFIIH in a physiological condition. Sepharose 77-86 general transcription factor IIH subunit 2 Homo sapiens 187-192 10706790-1 2000 Oligonucleotides bound by the CAAT enhancer binding protein (C/EBP), the lactose repressor, and Gal4 were chemically coupled to cyanogen bromide-activated Sepharose and the temperature dependence of transcription factor chromatography was characterized. Sepharose 155-164 CCAAT enhancer binding protein alpha Homo sapiens 61-66 10706790-1 2000 Oligonucleotides bound by the CAAT enhancer binding protein (C/EBP), the lactose repressor, and Gal4 were chemically coupled to cyanogen bromide-activated Sepharose and the temperature dependence of transcription factor chromatography was characterized. Sepharose 155-164 galectin 4 Homo sapiens 96-100 10669869-2 2000 Recombinant human heart chymase (rh-chymase) was purified from the culture medium via a single one-step heparin-agarose column chromatography tracing, using succinyl-Ala-Ala-Pro-Phe-para-nitroanilide (Suc-AAPF-pNA) hydrolysing activity. Sepharose 112-119 chymase 1 Homo sapiens 24-31 10699320-3 2000 The rLTB purified directly from the culture supernatant by using D-galactose immobilized agarose was identical to the native LTB with respect to the molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and the amino terminal amino acid sequence. Sepharose 89-96 lymphotoxin beta Rattus norvegicus 4-8 10698948-3 2000 Known cap-binding proteins, such as eIF4E and the nuclear cap-binding complex, are not detectable in the enzyme preparation, and PARN itself binds to m(7)GTP-Sepharose and is eluted specifically with the cap analog m(7)GTP. Sepharose 158-167 poly(A)-specific ribonuclease Homo sapiens 129-133 10710003-4 2000 Genotypes for the Spl COLIA1 polymorphism were determined by polymerase chain reaction, digestion with Ball restriction enzyme, and agarose gel electrophoresis. Sepharose 132-139 sphingosine-1-phosphate lyase 1 Homo sapiens 18-21 10688641-8 2000 Calmodulin seems to regulate the full activation of Raf-1 after Ras activation, since functional Ras is necessary for Raf-1 activation after nerve growth factor stimulation and calmodulin-Sepharose is able to precipitate Raf-1 in a calcium-dependent manner. Sepharose 188-197 calmodulin 1 Rattus norvegicus 0-10 10688641-8 2000 Calmodulin seems to regulate the full activation of Raf-1 after Ras activation, since functional Ras is necessary for Raf-1 activation after nerve growth factor stimulation and calmodulin-Sepharose is able to precipitate Raf-1 in a calcium-dependent manner. Sepharose 188-197 Raf-1 proto-oncogene, serine/threonine kinase Rattus norvegicus 52-57 10688641-8 2000 Calmodulin seems to regulate the full activation of Raf-1 after Ras activation, since functional Ras is necessary for Raf-1 activation after nerve growth factor stimulation and calmodulin-Sepharose is able to precipitate Raf-1 in a calcium-dependent manner. Sepharose 188-197 Raf-1 proto-oncogene, serine/threonine kinase Rattus norvegicus 118-123 10688641-8 2000 Calmodulin seems to regulate the full activation of Raf-1 after Ras activation, since functional Ras is necessary for Raf-1 activation after nerve growth factor stimulation and calmodulin-Sepharose is able to precipitate Raf-1 in a calcium-dependent manner. Sepharose 188-197 calmodulin 1 Rattus norvegicus 177-187 10688641-8 2000 Calmodulin seems to regulate the full activation of Raf-1 after Ras activation, since functional Ras is necessary for Raf-1 activation after nerve growth factor stimulation and calmodulin-Sepharose is able to precipitate Raf-1 in a calcium-dependent manner. Sepharose 188-197 Raf-1 proto-oncogene, serine/threonine kinase Rattus norvegicus 118-123 10671554-9 2000 Proteolytic cleavage of GST-GLTP fusion protein (bound to GST-Sepharose) and affinity purification resulted in fully active GLTP. Sepharose 62-71 glycolipid transfer protein Bos taurus 28-32 10772242-6 2000 In agarose cultures of bovine and porcine chondrocytes HAS2 mRNA was present in control, IL-1 and retinoic acid treated cultures, whereas HAS3 mRNA was only detected in IL-1 stimulated cultures. Sepharose 3-10 hyaluronan synthase 2 Bos taurus 55-59 10671493-8 2000 Further, when the leukemic cell line K562 was treated with c-myb RiAS oligos, colony formation on soft agarose was reduced by 92 +/- 2%. Sepharose 103-110 MYB proto-oncogene, transcription factor Homo sapiens 59-64 11776655-2 2000 METHODS: Cytosolic hsp70 protein of heat treated H22 cells was purified successively by chromatographic procedures, including DEAE 52-cellulose, ConA-sepharose 4B and ADP-argarose chromatography. Sepharose 150-162 heat shock protein 1B Mus musculus 19-24 10681564-3 2000 Under conditions in which avidin-Sepharose beads specifically adsorbed biotinylated Atp11p from yeast mitochondrial extracts, the F(1) beta-subunit coprecipitated with the tagged Atp11p protein. Sepharose 33-42 Atp11p Saccharomyces cerevisiae S288C 84-90 10681564-3 2000 Under conditions in which avidin-Sepharose beads specifically adsorbed biotinylated Atp11p from yeast mitochondrial extracts, the F(1) beta-subunit coprecipitated with the tagged Atp11p protein. Sepharose 33-42 Atp11p Saccharomyces cerevisiae S288C 179-185 10671554-9 2000 Proteolytic cleavage of GST-GLTP fusion protein (bound to GST-Sepharose) and affinity purification resulted in fully active GLTP. Sepharose 62-71 glycolipid transfer protein Bos taurus 124-128 10716622-3 2000 Agarose beads were soaked in a 500 ng/ml or 100 ng/ml solution of BMP-7 in culture medium and placed on the odontoblast area of the dentine pulp complex of rat tooth slices. Sepharose 0-7 bone morphogenetic protein 7 Rattus norvegicus 66-71 10660601-6 2000 Both constructs bound to calmodulin-Sepharose. Sepharose 36-45 calmodulin 1 Rattus norvegicus 25-35 10642604-2 2000 In the present work, gal-1 has been identified to be a ligand for the CD3-complex as well as for CD2 as detected by affinity chromatography of Jurkat T-cell lysates on gal-1 agarose and by binding of the biotinylated lectin to CD3 and CD2 immunoprecipitates on blots. Sepharose 174-181 galectin 1 Homo sapiens 21-26 10669644-7 2000 Agarose gel electrophoresis revealed that human LCAT gene transfer into human apoAI-transgenic mice resulted in an increase of pre-beta-HDL and of pre-alpha-HDL. Sepharose 0-7 lecithin-cholesterol acyltransferase Homo sapiens 48-52 10669644-7 2000 Agarose gel electrophoresis revealed that human LCAT gene transfer into human apoAI-transgenic mice resulted in an increase of pre-beta-HDL and of pre-alpha-HDL. Sepharose 0-7 apolipoprotein A1 Homo sapiens 78-83 10642604-2 2000 In the present work, gal-1 has been identified to be a ligand for the CD3-complex as well as for CD2 as detected by affinity chromatography of Jurkat T-cell lysates on gal-1 agarose and by binding of the biotinylated lectin to CD3 and CD2 immunoprecipitates on blots. Sepharose 174-181 CD2 molecule Homo sapiens 97-100 10705228-3 2000 The anti-rspg41 antisera after adsorption by IFN-beta sepharose column lost the activity of interaction with both synthetic peptides. Sepharose 54-63 interferon beta 1 Homo sapiens 45-53 10670468-8 2000 Agarose electrophoresis confirmed the presence of MUC1 and MUC4 and the absence of MUC2 or MUC5AC. Sepharose 0-7 mucin 1, cell surface associated Homo sapiens 50-54 10670468-8 2000 Agarose electrophoresis confirmed the presence of MUC1 and MUC4 and the absence of MUC2 or MUC5AC. Sepharose 0-7 mucin 4, cell surface associated Homo sapiens 59-63 10705228-4 2000 In another experiment, rsgp41 could bind to sepharose column conjugated with anti-IFN-beta polyclonal antibodies (IgG). Sepharose 44-53 interferon beta 1 Homo sapiens 82-90 10644679-3 2000 Both long and short fusion proteins retained the ability to bind CaM in a Ca(2+)-dependent manner as measured by CaM-Sepharose chromatography or a dansyl-CaM fluorescence assay. Sepharose 117-126 calmodulin 1 Homo sapiens 65-68 10681410-18 2000 This is different for LPL: mutations in the C-terminal binding domain (Cluster 4) cause a more significant shift in the salt required for elution from heparin-Sepharose than mutations in the N-terminal domain (Cluster 1). Sepharose 159-168 LOW QUALITY PROTEIN: lipoprotein lipase Cricetulus griseus 22-25 11272902-6 2000 As expected, IgH PCR assays produced diffuse smears in agarose gels or complex ladders in polyacrylamide gels when polyclonal B cell controls (blood and tonsil) were analyzed. Sepharose 55-62 immunoglobulin heavy constant delta Homo sapiens 13-16 10648172-12 2000 We conclude that the S-Sepharose included in the culture medium captures the BPI protein products as they are secreted and protects them from degradation and/or irreversible binding to cell surfaces. Sepharose 23-32 LOW QUALITY PROTEIN: bactericidal permeability-increasing protein Cricetulus griseus 77-80 10644679-3 2000 Both long and short fusion proteins retained the ability to bind CaM in a Ca(2+)-dependent manner as measured by CaM-Sepharose chromatography or a dansyl-CaM fluorescence assay. Sepharose 117-126 calmodulin 1 Homo sapiens 113-116 10644679-3 2000 Both long and short fusion proteins retained the ability to bind CaM in a Ca(2+)-dependent manner as measured by CaM-Sepharose chromatography or a dansyl-CaM fluorescence assay. Sepharose 117-126 calmodulin 1 Homo sapiens 113-116 10644679-5 2000 In addition, the binding of the short form to CaM-Sepharose was inhibited by phosphorylation with protein kinase A, whereas the binding of the long form was unaffected. Sepharose 50-59 calmodulin 1 Homo sapiens 46-49 10623479-8 2000 However, following immunoaffinity purification on a laminin beta1 antibody-Sepharose column, a 200-kDa band was readily detected by the antibodies to laminin alpha4 chain. Sepharose 75-84 laminin subunit beta 1 Homo sapiens 52-65 10640617-9 2000 An ATP-binding property for torsinA has been suggested by its homology to ATP-binding proteins; this was confirmed by enrichment of torsinA in ATP-agarose affinity-purified fractions from tissue homogenates. Sepharose 147-154 torsin family 1, member A Rattus norvegicus 28-35 10640617-9 2000 An ATP-binding property for torsinA has been suggested by its homology to ATP-binding proteins; this was confirmed by enrichment of torsinA in ATP-agarose affinity-purified fractions from tissue homogenates. Sepharose 147-154 torsin family 1, member A Rattus norvegicus 132-139 10673394-2 2000 Supershifting of p53-DNA complexes by MAbs in agarose gels was applied to studies of activation of p53 for sequence-specific binding within scDNA. Sepharose 46-53 tumor protein p53 Homo sapiens 17-20 10673394-2 2000 Supershifting of p53-DNA complexes by MAbs in agarose gels was applied to studies of activation of p53 for sequence-specific binding within scDNA. Sepharose 46-53 tumor protein p53 Homo sapiens 99-102 10620355-3 2000 XOR was purified as the NAD(+)-dependent dehydrogenase by benzamidine-Sepharose chromatography and was shown to be intact and to have biochemical properties similar to those of enzyme from other sources. Sepharose 70-79 xanthine dehydrogenase Homo sapiens 0-3 10625674-1 2000 A novel eosinophil chemotactic cytokine (ECF-L) was purified from the culture supernatant of splenocytes of mice by a combination of anion-exchange chromatography, Procion red-agarose affinity chromatography, size exclusion high performance liquid chromatography (HPLC), and reverse phase HPLC. Sepharose 176-183 chitinase-like 3 Mus musculus 41-46 10623479-8 2000 However, following immunoaffinity purification on a laminin beta1 antibody-Sepharose column, a 200-kDa band was readily detected by the antibodies to laminin alpha4 chain. Sepharose 75-84 laminin subunit alpha 4 Homo sapiens 150-170 10617640-3 2000 After affinity purification of protein A-tagged Gsp1p or Gsp2p by IgG-Sepharose chromatography, known karyopherin beta transport receptors (e.g. Kap121p and Kap123p) and a novel member of this protein family, Pdr6p, were found to be associated with yeast Ran. Sepharose 70-79 Ran GTPase GSP1 Saccharomyces cerevisiae S288C 48-53 10634811-2 2000 VLDL was oxidized for a maximum of 4 hours, resulting in an increase in thiobarbituric acid-reactive substances and an increased electrophoretic mobility on agarose gel. Sepharose 157-164 CD320 antigen Mus musculus 0-4 10645906-7 2000 Thus instillation of agarose plugs induces profound goblet cell metaplasia by causing EGFR expression and activation. Sepharose 21-28 epidermal growth factor receptor Rattus norvegicus 86-90 10601851-7 2000 Using reverse transcriptase PCR, mRNAs corresponding to the thrombin and fragment 1 + 2 (F1+2) regions of prothrombin were analysed by agarose gel electrophoresis. Sepharose 135-142 coagulation factor II Rattus norvegicus 106-117 12716306-14 2000 A significant reduction of SMBP-mediated DNA damage by Pcs was confirmed on agarose electrophoresis. Sepharose 76-83 transmembrane 9 superfamily member 3 Homo sapiens 27-31 16232878-6 2000 The renatured midkine was recovered using a SP-Sepharose column, and purified further by Heparin-Sepharose column chromatography. Sepharose 47-56 midkine Homo sapiens 14-21 10810223-4 2000 A Sepharose 4B column conjugated with 1 of the 4 antibodies that had inhibitory activity effectively adsorbed and eluted the DNase I enzyme; this did not occur with the rabbit polyclonal antibody. Sepharose 2-11 deoxyribonuclease I Mus musculus 125-132 11920189-4 2000 We determined the range of vWF concentrations in plasma where the percentage of (125)I-MAb/vWF complexes bound to heparin-agarose beads was constant. Sepharose 122-129 von Willebrand factor Homo sapiens 27-30 10591628-3 2000 Microcystin-Sepharose affinity purification revealed a prominent 35-kDa axonemal protein, predicted to be the catalytic subunit of type-1 protein phosphatase (PP1c). Sepharose 12-21 protein phosphatase 1 catalytic subunit gamma Homo sapiens 159-163 16232878-6 2000 The renatured midkine was recovered using a SP-Sepharose column, and purified further by Heparin-Sepharose column chromatography. Sepharose 97-106 midkine Homo sapiens 14-21 10687142-1 2000 An interferon-gamma (IFN-gamma)-inducing molecule (OK-PSA) has been purified from OK-432 by an affinity chromatographic technique performed on cyanogen bromide-activated Sepharose 4B-bound TS-2 monoclonal antibody, which neutralizes IFN-gamma-inducing activity of OK-432. Sepharose 170-182 interferon gamma Mus musculus 3-19 10687142-1 2000 An interferon-gamma (IFN-gamma)-inducing molecule (OK-PSA) has been purified from OK-432 by an affinity chromatographic technique performed on cyanogen bromide-activated Sepharose 4B-bound TS-2 monoclonal antibody, which neutralizes IFN-gamma-inducing activity of OK-432. Sepharose 170-182 interferon gamma Mus musculus 21-30 11188946-7 2000 A portion of CCR-5 gene from genomic DNA was amplified by PCR and analyzed on a 3% agarose gel. Sepharose 83-90 C-C motif chemokine receptor 5 Homo sapiens 13-18 10617137-8 2000 A similar difference in the inhibitory potential of the antibody was observed between fractions of isolated 5"-nucleotidase binding to concanavalin A-Sepharose (70%) and fractions not retained by the lectin column (18%). Sepharose 150-159 5' nucleotidase, ecto Rattus norvegicus 108-123 12075418-1 2000 An acidic phospholipase A(2) from Agkistrondon acutus venom has been purified to homogeneity via four steps using CM-Sepharose, two times DEAE-Sepharose, and Mono Q FPLC. Sepharose 117-126 phospholipase A2 group IB Homo sapiens 10-28 11831986-3 2000 The constructed eukaryorotic expression vectors containing the human CD(59) cDNA, LXSN-CD(59) were identified by the methods of restriction endonucleases cleavage, agarose gel electrophoresis, and polymerase chain reaction. Sepharose 164-171 CD59 molecule (CD59 blood group) Homo sapiens 69-75 12110906-5 2000 The antiserum showed specificity to hB1F after the purification by GST-Sepharose 4B. Sepharose 71-80 nuclear receptor subfamily 5 group A member 2 Homo sapiens 36-40 10606531-7 1999 When we added SAA to a column of Sepharose beads coupled to the isolated HDL (alpha-migrating HDL), prebeta1-HDL was dissociated from the column together with the SAA-associated HDL. Sepharose 33-42 serum amyloid A1 Homo sapiens 14-17 10585296-4 1999 Immunoaffinity purification on a laminin beta1 antibody-Sepharose column yielded polypeptides of 230, 220, 200, and 180 kDa from platelet lysates. Sepharose 56-65 laminin subunit beta 1 Homo sapiens 33-46 10607589-6 1999 Both proteins were isolated by affinity chromatography on uPA-conjugated cyanogen-bromide-activated Sepharose 4B and were identified using nano-electrospray mass spectrometry and immunoblotting. Sepharose 100-109 plasminogen activator, urokinase Homo sapiens 58-61 10572250-6 1999 We also found that polyhistidine-tagged rap2 immobilized on Ni(2+)-Sepharose and loaded with either GDP or GTPgammaS, could specifically bind to cytoskeletal actin. Sepharose 67-76 RAP2A, member of RAS oncogene family Homo sapiens 40-44 10601500-5 1999 Immunoblotting of extracts from freshly isolated myometrial tissue, affinity-enriched for NOS proteins by precipitation with ADP-sepharose, revealed expression of endothelial NOS (eNOS or NOS3) in tissues from preterm, term non-labour and active labour at term. Sepharose 129-138 nitric oxide synthase 3 Homo sapiens 180-184 10616011-6 1999 RESULTS: A total of 48 synovial explants were cultured in agarose with TGFbeta1. Sepharose 58-65 transforming growth factor, beta 1 Mus musculus 71-79 10648963-4 1999 The recombinant Ra-eRF1 was marked with a histidine tag, overexpressed, and purified to homogeneity by two-step chromatography using Ni-NTA-agarose and Mono Q columns. Sepharose 140-147 eukaryotic peptide chain release factor subunit 1 Oryctolagus cuniculus 19-23 10585350-3 1999 METHODS: beta-Glucuronidase (GRD; EC 3.2.1.31) and arylsulfatase (ARS; EC 3.1.6.1) were purified and coimmobilized on an agarose gel matrix and packed into columns. Sepharose 121-128 glucuronidase beta Homo sapiens 9-27 10585350-3 1999 METHODS: beta-Glucuronidase (GRD; EC 3.2.1.31) and arylsulfatase (ARS; EC 3.1.6.1) were purified and coimmobilized on an agarose gel matrix and packed into columns. Sepharose 121-128 glucuronidase beta Homo sapiens 29-32 10669049-4 1999 It was observed that suramin competed with heparin-Sepharose gel for binding to chymase and the inhibition of chymase activity by suramin was partially impaired by heparin. Sepharose 51-60 chymase 1 Homo sapiens 80-87 10599977-6 1999 Moreover, qualitative assessment of the induced DNA damage on agarose gels showed that 10 nmol/L TNF-alpha produced characteristic apoptotic patterns of DNA fragments formed by internucleosomal hydrolysis of static chromatin. Sepharose 62-69 tumor necrosis factor Mus musculus 97-106 10570059-4 1999 The trapped in agarose DNA immunostaining assay (TARDIS) was used to detect topo IIalpha and beta cleavable complexes in topo IIbeta -/- and topo IIbeta +/+ cells. Sepharose 15-22 ATPase, class II, type 9A Mus musculus 81-88 10570059-4 1999 The trapped in agarose DNA immunostaining assay (TARDIS) was used to detect topo IIalpha and beta cleavable complexes in topo IIbeta -/- and topo IIbeta +/+ cells. Sepharose 15-22 ATPase, class II, type 9B Mus musculus 126-132 10570059-4 1999 The trapped in agarose DNA immunostaining assay (TARDIS) was used to detect topo IIalpha and beta cleavable complexes in topo IIbeta -/- and topo IIbeta +/+ cells. Sepharose 15-22 ATPase, class II, type 9B Mus musculus 146-152 10581349-2 1999 METHODS: YAP element was detected by PCR amplification and agarose gel electrophoresis. Sepharose 59-66 Yes1 associated transcriptional regulator Homo sapiens 9-12 10594362-8 1999 Using RT-PCR assay with several specific human SP primer pairs, we were able to differentiate four isoforms of preprotachykinin (PPT-A, the SP precursor) mRNA transcripts on ethidium bromide-stained agarose gels and clone the PCR amplified cDNA of the four isoforms (alpha, beta, gamma, and delta) of the PPT-A gene. Sepharose 199-206 tachykinin precursor 1 Homo sapiens 111-127 10594362-8 1999 Using RT-PCR assay with several specific human SP primer pairs, we were able to differentiate four isoforms of preprotachykinin (PPT-A, the SP precursor) mRNA transcripts on ethidium bromide-stained agarose gels and clone the PCR amplified cDNA of the four isoforms (alpha, beta, gamma, and delta) of the PPT-A gene. Sepharose 199-206 tachykinin precursor 1 Homo sapiens 129-134 10545219-2 1999 XOR was purified 80-fold in two steps using benzamidine-Sepharose affinity chromatography. Sepharose 56-65 xanthine dehydrogenase Mus musculus 0-3 10553088-5 1999 Proteins eluted from a galectin-3-Sepharose column by lactose were analyzed on SDS-polyacrylamide gels and showed two major bands of 100 and 160 kDa and a minor band of 120 kDa. Sepharose 34-43 galectin 3 Homo sapiens 23-33 10551888-2 1999 By affinity chromatography with p21(Cip1)-Sepharose 4B columns, we purified a 39-kDa protein, which was identified by microsequence analysis as the oncoprotein SET. Sepharose 42-51 cyclin dependent kinase inhibitor 1A Homo sapiens 36-40 10684259-8 2000 Furthermore, the expression of occludin inhibited anchorage-independent growth of Raf-1-activated Pa-4 cells in soft agarose. Sepharose 117-124 occludin Rattus norvegicus 31-39 10684259-8 2000 Furthermore, the expression of occludin inhibited anchorage-independent growth of Raf-1-activated Pa-4 cells in soft agarose. Sepharose 117-124 Raf-1 proto-oncogene, serine/threonine kinase Rattus norvegicus 82-87 10583216-5 1999 Furthermore, agarose beads coated with PF4 alone were almost as effective as beads coated with PF4 plus heparin in depleting HIT plasmas of anti-PF4-heparin antibodies. Sepharose 13-20 platelet factor 4 Homo sapiens 39-42 10583216-7 1999 Binding of PF4 to heparin (optimal) or polystyrene/agarose (suboptimal) promotes recognition of this epitope. Sepharose 51-58 platelet factor 4 Homo sapiens 11-14 10502299-8 1999 Mutation of either Tyr851 or Tyr883, but mostly the latter, decreased Src phosphorylation of cadherin and the binding of Shc to cadherin, as determined by Sepharose bead binding and gel overlay assays. Sepharose 155-164 SHC adaptor protein 1 Homo sapiens 121-124 10527938-2 1999 In this study, we adapted a deoxyribonuclease I-Sepharose affinity purification scheme and we were able to enrich and isolate multiple functional plant actin isovariants from common bean leaves (Phaseolus vulgaris). Sepharose 48-57 actin Oryctolagus cuniculus 152-157 10589788-5 1999 Purified K1-3 protein is apparently folded in an active conformation, as evidenced by its ability to bind to lysine-Sepharose. Sepharose 116-125 keratin 13 Homo sapiens 9-13 10599999-4 1999 The appearance of CD14 on lymphocytes was also elicited by exposure of PBMC to phytohemagglutinin (PHA), concanavalin A (Con A), or agarose-bound phytohemagglutinin but not by exposure to lipopolysaccharide, interferon-alpha, or interleukin-2. Sepharose 132-139 CD14 molecule Homo sapiens 18-22 10599999-4 1999 The appearance of CD14 on lymphocytes was also elicited by exposure of PBMC to phytohemagglutinin (PHA), concanavalin A (Con A), or agarose-bound phytohemagglutinin but not by exposure to lipopolysaccharide, interferon-alpha, or interleukin-2. Sepharose 132-139 interleukin 2 Homo sapiens 229-242 10527657-2 1999 The expression of iNOS mRNA in the astrocytes was inhibited by LY294002, as revealed by reverse transcriptional polymerase chain reaction and agarose gel electrophoresis. Sepharose 142-149 nitric oxide synthase 2, inducible Mus musculus 18-22 10541273-8 1999 Using reverse transcription-PCR, mRNA corresponding to the thrombin and F1+2 regions of PT was analyzed by agarose gel electrophoresis. Sepharose 107-114 coagulation factor II, thrombin Homo sapiens 59-67 10541273-8 1999 Using reverse transcription-PCR, mRNA corresponding to the thrombin and F1+2 regions of PT was analyzed by agarose gel electrophoresis. Sepharose 107-114 coagulation factor II, thrombin Homo sapiens 88-90 10537071-5 1999 The binding of zinc by the small myelin-associated glycoprotein induces a conformational change that enables the protein to reversibly bind to a hydrophobic phenyl-Sepharose matrix. Sepharose 164-173 myelin associated glycoprotein Homo sapiens 33-63 10590683-3 1999 The detective sensitivity of the IgH-PCR was different dependently upon each analysis for amplified products, namely 10(-2) per mononuclear cells in agarose gel analysis and 10(-3) in polyacrylamide gel and single strand conformation polymorphism analysis (PAGE and SSCP). Sepharose 149-156 immunoglobulin heavy locus Homo sapiens 33-36 10526902-7 1999 Cdk2 activity was determined by autoradiography after polyacrylamide gel electrophoresis of VSMC extracts that had been immunoprecipitated with anti-cdk2 antibody and protein A sepharose, and then incubated with 32P-ATP and histone H1. Sepharose 177-186 cyclin dependent kinase 2 Rattus norvegicus 0-4 10933056-9 1999 R/R-BRCA1 cells generated approximately 5 to 10 times less colonies in a soft-agarose assay compared to the negative control. Sepharose 78-85 BRCA1, DNA repair associated Rattus norvegicus 4-9 10529380-2 1999 In order to examine the presence of the specific binding protein to the sticky IgA1 in human serum, IgA1, asialo-IgA1 (IgA1-S) and asialo-, agalacto-IgA1 (IgA1-SG)/Sepharose column chromatography of normal human serum was carried out. Sepharose 164-173 immunoglobulin heavy constant alpha 1 Homo sapiens 79-83 10529380-3 1999 Purified hinge glycopeptide (HGP33) prepared from IgA1 was used for the preparation of HGP/Sepharose. Sepharose 91-100 immunoglobulin heavy constant alpha 1 Homo sapiens 50-54 10529380-5 1999 About four times the amount of protein was eluted from the IgA1-SG/Sepharose column than that from IgA1/Sepharose. Sepharose 67-76 immunoglobulin heavy constant alpha 1 Homo sapiens 59-63 10596471-5 1999 METHODS AND RESULTS: Our diagnosis is based on the differentiation of normal and mutant alleles of gene X25 with PCR and electrophoresis on agarose gel. Sepharose 140-147 frataxin Homo sapiens 104-107 10510350-8 1999 Of note, CD154 itself not only functioned as a ligand but also as a direct signaling molecule as anti-CD154-conjugated Sepharose beads costimulated B cell responses induced by engaging surface Ig. Sepharose 119-128 CD40 ligand Homo sapiens 9-14 10510350-8 1999 Of note, CD154 itself not only functioned as a ligand but also as a direct signaling molecule as anti-CD154-conjugated Sepharose beads costimulated B cell responses induced by engaging surface Ig. Sepharose 119-128 CD40 ligand Homo sapiens 102-107 10504408-7 1999 PC-1 was capable of binding to heparin-Sepharose and the binding was inhibited in the presence of the enzyme substrate, ATP or its nonhydrolyzable analog. Sepharose 39-48 ectonucleotide pyrophosphatase/phosphodiesterase 1 Homo sapiens 0-4 12666954-7 1999 Removal of lipopolysaccharide binding protein from either serum by adsorption to an anti lipopolysaccharide binding protein-sepharose affinity column removed the priming activity of normal neutrophils. Sepharose 124-133 lipopolysaccharide binding protein Homo sapiens 11-45 12666954-7 1999 Removal of lipopolysaccharide binding protein from either serum by adsorption to an anti lipopolysaccharide binding protein-sepharose affinity column removed the priming activity of normal neutrophils. Sepharose 124-133 lipopolysaccharide binding protein Homo sapiens 89-123 10863479-2 1999 Agarose gel electrophoresis of the PCR products of the cDNA generated from RNA was carried out to demonstrate the expression of mRNA in syndecan-1, syndecan-2, syndecan-4 and glypican in this study. Sepharose 0-7 syndecan 1 Homo sapiens 136-146 10863479-2 1999 Agarose gel electrophoresis of the PCR products of the cDNA generated from RNA was carried out to demonstrate the expression of mRNA in syndecan-1, syndecan-2, syndecan-4 and glypican in this study. Sepharose 0-7 syndecan 2 Homo sapiens 148-158 10863479-2 1999 Agarose gel electrophoresis of the PCR products of the cDNA generated from RNA was carried out to demonstrate the expression of mRNA in syndecan-1, syndecan-2, syndecan-4 and glypican in this study. Sepharose 0-7 syndecan 4 Homo sapiens 160-170 10863479-2 1999 Agarose gel electrophoresis of the PCR products of the cDNA generated from RNA was carried out to demonstrate the expression of mRNA in syndecan-1, syndecan-2, syndecan-4 and glypican in this study. Sepharose 0-7 glypican 1 Homo sapiens 175-183 10504266-8 1999 Phosphorylation of GST-neurabin I (residues 318-661) by PKA significantly reduced its binding to PP1 by overlay and by glutathione-Sepharose coprecipitation assays. Sepharose 131-140 protein phosphatase 1 regulatory subunit 9A Homo sapiens 23-33 10556557-4 1999 The present paper shows that modified P450scc, purified by affinity chromatography using adrenodoxin-Sepharose to remove non-covalently bound FITC, retains the functional activity of the unmodified enzyme, including its ability to bind adrenodoxin. Sepharose 101-110 cholesterol side-chain cleavage enzyme, mitochondrial Bos taurus 38-45 10478584-5 1999 A simple non-radioactive PCR for rapid detection of the expanded SCA2 alleles via agarose gel electrophoresis was also employed. Sepharose 82-89 ataxin 2 Homo sapiens 65-69 10536969-8 1999 Gradient elution on a benzamidine sepharose 6B column resulted however in a single, low yield (17.9%) tryptase peak and a broader, high yield (>90%) chymase peak, and a 34% yield high purity fraction. Sepharose 34-43 tryptase alpha/beta 1 Mus musculus 102-110 10484460-2 1999 Three peaks of PLA2 activity were identified by heparin-Sepharose chromatography. Sepharose 56-65 phospholipase A2 Ovis aries 15-19 10511040-3 1999 The coding regions of the CYP1B1 gene were amplified by polymerase chain reaction, examined by agarose gel separation and heteroduplex methods, and directly sequenced. Sepharose 95-102 cytochrome P450 family 1 subfamily B member 1 Homo sapiens 26-32 10511040-4 1999 RESULTS: One of the patients with primary infantile glaucoma had a mutation of the CYP1B1 gene, with an abnormal shift in agarose gel separation and heteroduplex analysis. Sepharose 122-129 cytochrome P450 family 1 subfamily B member 1 Homo sapiens 83-89 10505210-5 1999 Here, we describe a new method for haptoglobin phenotyping using commercially available agarose gels and a non-carcinogenic stain (3,3",5,5"-tetramethylbenzidine). Sepharose 88-95 haptoglobin Homo sapiens 35-46 10455014-4 1999 The recombinant rhodopsin could be purified with high overall yield by using immobilized-metal-affinity chromatography on Ni(2+)-agarose. Sepharose 129-136 rhodopsin Bos taurus 16-25 10513810-3 1999 METHODS: Chondrocytes from human articular cartilage, cultured in agarose, were stimulated with IL-6-type cytokines. Sepharose 66-73 interleukin 6 Homo sapiens 96-100 10469037-16 1999 Profilin of the extract was purified by poli-L-proline-Sepharose chromatography and it appeared as one of the most frequent allergens. Sepharose 55-64 profilin-1-like Ricinus communis 0-8 10994080-5 2000 According to the results of chromatography on anti-Hsp70 antibody-Sepharose gel, approximately 50% of the protein was retarded by the gel, while the other part was not recognized by immunoglobulins. Sepharose 66-75 heat shock protein family A (Hsp70) member 4 Homo sapiens 51-56 10461922-1 1999 We purified an 80-kDa Ca2+-independent phospholipase A2 (iPLA2) from rat brain using octyl-Sepharose, ATP-agarose, and calmodulin-agarose column chromatography steps. Sepharose 106-113 phospholipase A2 group VI Rattus norvegicus 22-55 10461922-1 1999 We purified an 80-kDa Ca2+-independent phospholipase A2 (iPLA2) from rat brain using octyl-Sepharose, ATP-agarose, and calmodulin-agarose column chromatography steps. Sepharose 106-113 phospholipase A2 group VI Rattus norvegicus 57-62 10432310-1 1999 The ability to separate the isoforms of human tumour suppressor protein p53 expressed in insect cells using heparin-Sepharose correlates with differences in the isoelectric point of p53, demonstrating that p53 can be heterogeneously modified and providing support for the use of insect cells as a model system for identifying novel signalling pathways that target p53. Sepharose 116-125 tumor protein p53 Homo sapiens 72-75 10441379-5 1999 After 24 h treatment with 25 micrograms/ml of ACL LAO, the typical DNA fragmentation pattern of apoptotic cells was observed on agarose gel electrophoresis. Sepharose 128-135 interleukin 4 induced 1 Homo sapiens 50-53 10446402-6 1999 The concentration of p21 from the cell lysate was performed using an anti-p21 antibody crosslinked to protein G Sepharose. Sepharose 112-121 cyclin dependent kinase inhibitor 1A Homo sapiens 21-24 10446402-6 1999 The concentration of p21 from the cell lysate was performed using an anti-p21 antibody crosslinked to protein G Sepharose. Sepharose 112-121 cyclin dependent kinase inhibitor 1A Homo sapiens 74-77 10425209-7 1999 In order to examine the nature of such a sticky IgA1, affinity chromatography using asialo-IgA1 (deSIgA1)-Sepharose was carried out. Sepharose 106-115 immunoglobulin heavy constant alpha 1 Homo sapiens 48-52 10425209-7 1999 In order to examine the nature of such a sticky IgA1, affinity chromatography using asialo-IgA1 (deSIgA1)-Sepharose was carried out. Sepharose 106-115 immunoglobulin heavy constant alpha 1 Homo sapiens 91-95 10430972-4 1999 The C677T transition in the MTHFR gene was detected by HinF 1 restriction enzyme analysis and subsequent electrophoresis in a 3% agarose gel. Sepharose 129-136 methylenetetrahydrofolate reductase Homo sapiens 28-33 10430974-3 1999 The ACE insertion/deletion (I/D) polymorphism was amplified with the previously published flanking primers, and the polymerase chain reaction product was separated, sized on a 2% agarose gel, and visualized by ultraviolet transillumination. Sepharose 179-186 angiotensin I converting enzyme Homo sapiens 4-7 10417337-2 1999 Myrosinase was purified to homogeneity in good yield from 8-day-old seedlings of Raphanus sativus (daikon) using a four-step procedure involving chromatographies on anion exchange, hydrophobic Phenyl-Sepharose, gel filtration and concanavalin A-Sepharose. Sepharose 200-209 myrosinase Raphanus sativus 0-10 10419879-5 1999 APC was also shown to bind to patient"s IgG immobilized on a protein A agarose column. Sepharose 71-78 APC regulator of WNT signaling pathway Homo sapiens 0-3 10456886-6 1999 Phase-contrast microscopy observations of P40-treated cells revealed morphological changes, including pronounced blebbing of the plasma membrane and cytoplasmic shrinkage characteristic of programmed cell death, which is in agreement with the internucleosomal fragmentation of P40-treated cell DNA as shown by agarose gel electrophoresis. Sepharose 310-317 interleukin 9 Homo sapiens 42-45 10362844-4 1999 Isopycnic density gradient centrifugation gave good resolution of mature and precursor forms of MUC2 as assessed by agarose gel electrophoresis. Sepharose 116-123 mucin 2, oligomeric mucus/gel-forming Homo sapiens 96-100 10362844-6 1999 Rate-zonal centrifugation and agarose electrophoretic analysis of the low-density fraction indicated that the N-glycosylated MUC2 polypeptide was present as putative monomer and dimer/oligomer species. Sepharose 30-37 mucin 2, oligomeric mucus/gel-forming Homo sapiens 125-129 10362844-7 1999 The combination of isopycnic density gradient centrifugation with agarose electrophoresis provides a new and simple approach that allows us to follow the MUC2 gene product from polypeptide through to the mature glycosylated mucin. Sepharose 66-73 mucin 2, oligomeric mucus/gel-forming Homo sapiens 154-158 10362844-7 1999 The combination of isopycnic density gradient centrifugation with agarose electrophoresis provides a new and simple approach that allows us to follow the MUC2 gene product from polypeptide through to the mature glycosylated mucin. Sepharose 66-73 LOC100508689 Homo sapiens 224-229 10393319-3 1999 Overproduction of AR triggered DNA fragmentation, as judged with the TUNEL method and agarose gel electrophoresis. Sepharose 86-93 aldo-keto reductase family 1 member B1 Rattus norvegicus 18-20 10404822-14 1999 After incubation of serum samples with L2 cell extracts, incubation with antihuman IgG Fc-specific agarose beads resulted in immunoprecipitation of megalin in all the 18 positive patients, but not in normal subjects, as assessed by Western blotting using a monoclonal antibody against megalin. Sepharose 99-106 LDL receptor related protein 2 Homo sapiens 148-155 10404822-14 1999 After incubation of serum samples with L2 cell extracts, incubation with antihuman IgG Fc-specific agarose beads resulted in immunoprecipitation of megalin in all the 18 positive patients, but not in normal subjects, as assessed by Western blotting using a monoclonal antibody against megalin. Sepharose 99-106 LDL receptor related protein 2 Homo sapiens 285-292 10393207-7 1999 Hemin-agarose affinity chromatography of the native RFABG followed by Western blot analysis showed a single immunoreactive band at 70 kDa, indicating that the heme-binding domain resides in the 70 kDa subunit. Sepharose 6-13 apolipophorin Drosophila melanogaster 52-57 10546279-1 1999 A simple and convenient method of simultaneous determinations of effects of any set of chemical compounds on enzymatic hydrolysis of phospholipids by using the method of diffusion phospholipase A2 in a lecithin-agarose gel. Sepharose 211-218 phospholipase A2 group IB Homo sapiens 180-196 10373411-4 1999 We report here the expression in Sf9 cells of glutathione S-transferase-tagged recombinant human sGCalpha1 and beta1 subunits, applying a novel and rapid purification method based on GSH-Sepharose affinity chromatography. Sepharose 187-196 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 111-116 10382588-5 1999 METHODS: Length variation of the UCP2 exon 8 variant was studied by the polymerase chain reaction and agarose gel electrophoresis. Sepharose 102-109 uncoupling protein 2 Homo sapiens 33-37 10352300-3 1999 Neutrophil migration in response to fMLP was assessed using an agarose overlay method with slides precoated with fibronectin (Fn) +/- beta-glucan. Sepharose 63-70 formyl peptide receptor 1 Homo sapiens 36-40 10459873-5 1999 METHODS: Maternal and fetal samples were genotyped at the insertion-deletion (I-D) polymorphism in intron 16 of the angiotensin-converting enzyme gene by the polymerase chain reaction followed by agarose electrophoresis. Sepharose 196-203 angiotensin I converting enzyme Homo sapiens 116-145 10533721-6 1999 Agarose gel electrophoresis confirmed the AdCMV.p53-dependent cellular apoptosis. Sepharose 0-7 tumor protein p53 Homo sapiens 48-51 10336866-7 1999 The SSB protein was purified by metal-affinity chromatography on Ni2+-TED-Sepharose columns. Sepharose 74-83 single-stranded DNA-binding protein Escherichia coli 4-7 10418058-4 1999 METHODS: Ro60 ribonucleoprotein was affinity-purified from human spleen extracts using 4B-Sepharose linked to anti-Ro60 monoclonal antibodies. Sepharose 90-99 Ro60, Y RNA binding protein Homo sapiens 9-13 10336486-7 1999 Agarose gel electrophoresis of subunits from individuals expressing both a "long" and a "short" MUC2 allele revealed a larger number of populations, consistent with the presence of short and long monomers and oligomers arising from permutations of the two types of monomers. Sepharose 0-7 mucin 2, oligomeric mucus/gel-forming Homo sapiens 96-100 10412842-7 1999 Agarose gel electrophoresis of the RT-PCR products from the ganglia demonstrated the presence of mRNA corresponding to CGRP1, NPY Y1 and Y2, and VIP1 receptors. Sepharose 0-7 calcitonin related polypeptide alpha Homo sapiens 119-124 10412842-7 1999 Agarose gel electrophoresis of the RT-PCR products from the ganglia demonstrated the presence of mRNA corresponding to CGRP1, NPY Y1 and Y2, and VIP1 receptors. Sepharose 0-7 diphosphoinositol pentakisphosphate kinase 1 Homo sapiens 145-149 10328774-4 1999 The in vivo biotinylated luciferase and oxidoreductase were immobilized on avidin-conjugated agarose beads with little loss of activity. Sepharose 93-100 oxidoreductase Escherichia coli 40-54 10318819-7 1999 NIPP-1(191-210) competed for PP-1 inhibition by full-length NIPP-1(1-351), inhibitor-1 and inhibitor-2, and dissociated PP-1C from inhibitor-1- and NIPP-1(143-217)-Sepharose but not from full-length NIPP-1(1-351)-Sepharose. Sepharose 164-173 protein phosphatase 1 regulatory subunit 8 Homo sapiens 0-6 10318819-7 1999 NIPP-1(191-210) competed for PP-1 inhibition by full-length NIPP-1(1-351), inhibitor-1 and inhibitor-2, and dissociated PP-1C from inhibitor-1- and NIPP-1(143-217)-Sepharose but not from full-length NIPP-1(1-351)-Sepharose. Sepharose 164-173 inorganic pyrophosphatase 1 Homo sapiens 2-6 10318819-7 1999 NIPP-1(191-210) competed for PP-1 inhibition by full-length NIPP-1(1-351), inhibitor-1 and inhibitor-2, and dissociated PP-1C from inhibitor-1- and NIPP-1(143-217)-Sepharose but not from full-length NIPP-1(1-351)-Sepharose. Sepharose 213-222 protein phosphatase 1 regulatory subunit 8 Homo sapiens 0-6 10318819-7 1999 NIPP-1(191-210) competed for PP-1 inhibition by full-length NIPP-1(1-351), inhibitor-1 and inhibitor-2, and dissociated PP-1C from inhibitor-1- and NIPP-1(143-217)-Sepharose but not from full-length NIPP-1(1-351)-Sepharose. Sepharose 213-222 inorganic pyrophosphatase 1 Homo sapiens 2-6 10329602-8 1999 Finally, we demonstrated that authentic full-length human ADAM 12 could bind to heparin Sepharose. Sepharose 88-97 ADAM metallopeptidase domain 12 Homo sapiens 58-65 10212199-4 1999 When vWf was immobilized on agarose-linked monoclonal antibody, factor VIII bound to vWf with high affinity, and neither the affinity nor binding site availability was influenced by the presence of 50% plasma. Sepharose 28-35 von Willebrand factor Homo sapiens 5-8 10212199-4 1999 When vWf was immobilized on agarose-linked monoclonal antibody, factor VIII bound to vWf with high affinity, and neither the affinity nor binding site availability was influenced by the presence of 50% plasma. Sepharose 28-35 von Willebrand factor Homo sapiens 85-88 10212199-6 1999 In contrast, when vWf was immobilized on agarose-linked collagen, its affinity for factor VIII was reduced 4-fold, with KD increasing from 0.9 to 3.8 nM. Sepharose 41-48 von Willebrand factor Homo sapiens 18-21 10206965-5 1999 Wild-type CPE and the Gln300 point mutant bound to a p-aminobenzoyl-Arg-Sepharose affinity resin, and this binding was competed by an active site-directed inhibitor, guanidinoethylmercaptosuccinic acid. Sepharose 72-81 carboxypeptidase E Rattus norvegicus 10-13 10206989-3 1999 In this study, we have purified an nNOS-interacting protein from bovine brain using an affinity column made of Sepharose conjugated with glutathione S-transferase-rat nNOS fusion protein and identified it as alpha1-syntrophin by microsequencing. Sepharose 111-120 nitric oxide synthase 1 Rattus norvegicus 35-39 10206989-3 1999 In this study, we have purified an nNOS-interacting protein from bovine brain using an affinity column made of Sepharose conjugated with glutathione S-transferase-rat nNOS fusion protein and identified it as alpha1-syntrophin by microsequencing. Sepharose 111-120 nitric oxide synthase 1 Rattus norvegicus 167-171 10206989-3 1999 In this study, we have purified an nNOS-interacting protein from bovine brain using an affinity column made of Sepharose conjugated with glutathione S-transferase-rat nNOS fusion protein and identified it as alpha1-syntrophin by microsequencing. Sepharose 111-120 syntrophin, alpha 1 Rattus norvegicus 208-225 10327168-1 1999 Mouse plasma acetylcholinesterase (AChE) tetramers (G4) and dimers (G2) were retained by edrophonium-Sepharose, whereas AChE monomers (G1), and G4, G2 and G1 butyrylcholinesterase (BuChE) forms were not. Sepharose 101-110 acetylcholinesterase Mus musculus 13-33 10327168-1 1999 Mouse plasma acetylcholinesterase (AChE) tetramers (G4) and dimers (G2) were retained by edrophonium-Sepharose, whereas AChE monomers (G1), and G4, G2 and G1 butyrylcholinesterase (BuChE) forms were not. Sepharose 101-110 acetylcholinesterase Mus musculus 35-39 10196161-7 1999 The isolation of complexes between TIMP-3 and progelatinases A and B on gelatin-agarose demonstrated that TIMP-3 binds to both proenzymes. Sepharose 80-87 TIMP metallopeptidase inhibitor 3 Homo sapiens 35-41 10196161-7 1999 The isolation of complexes between TIMP-3 and progelatinases A and B on gelatin-agarose demonstrated that TIMP-3 binds to both proenzymes. Sepharose 80-87 matrix metallopeptidase 2 Homo sapiens 46-68 10196161-7 1999 The isolation of complexes between TIMP-3 and progelatinases A and B on gelatin-agarose demonstrated that TIMP-3 binds to both proenzymes. Sepharose 80-87 TIMP metallopeptidase inhibitor 3 Homo sapiens 106-112 10217155-1 1999 The separation of complement factors C3 and C3b by isotachophoresis in 1% agarose gel followed by immunoprecipitation and quantification is presented. Sepharose 74-81 complement C3 Homo sapiens 44-47 10217162-0 1999 Human dopamine D4 receptor allele genotyping by ultrathin agarose gel electrophoresis with To-Pro-3 complexation. Sepharose 58-65 dopamine receptor D4 Homo sapiens 6-26 10217162-2 1999 A sensitive, ultrathin agarose gel electrophoresis-based, high-throughput screening method was developed for genotyping the dopamine D4 receptor (D4DR) exon III 48 base pair repeat polymorphism. Sepharose 23-30 dopamine receptor D4 Homo sapiens 124-144 10217162-2 1999 A sensitive, ultrathin agarose gel electrophoresis-based, high-throughput screening method was developed for genotyping the dopamine D4 receptor (D4DR) exon III 48 base pair repeat polymorphism. Sepharose 23-30 dopamine receptor D4 Homo sapiens 146-150 10024667-6 1999 Western blots of agarose gel electrophoresis of fractions across the anion exchange distribution indicated that the polypeptide underlying the mucins was the product of the MUC5B gene. Sepharose 17-24 mucin 5B, oligomeric mucus/gel-forming Homo sapiens 173-178 10051489-5 1999 Glucocorticoid receptor (GR) can be precipitated with the fusion protein, GST-c-Jun (1-79), bound to agarose beads. Sepharose 101-108 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 0-23 10051489-5 1999 Glucocorticoid receptor (GR) can be precipitated with the fusion protein, GST-c-Jun (1-79), bound to agarose beads. Sepharose 101-108 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 25-27 12205917-5 1999 The results showed that stimulating index (SI) of MLIC and IL-2 level in the macroencapsulated islet xenografts in agarose group were similar to those of syngenic pancreatic islets group. Sepharose 115-122 interleukin 2 Homo sapiens 59-63 10331242-3 1999 In this context, the mineralization potential of fibronectin was tested in an agarose gel precipitation system and a metastable calcium phosphate solution. Sepharose 78-85 fibronectin 1 Homo sapiens 49-60 10199223-6 1999 Presence of precursor and mature forms of cathepsin D in vaginal wash was demonstrated after passage through a pepstatin A-agarose column. Sepharose 123-130 cathepsin D Homo sapiens 42-53 10026282-3 1999 Lp(a) binds lysine-Sepharose via a lysine binding site (LBS) located in KIV-10 (88% homology with plasminogen K4). Sepharose 19-28 lipoprotein(a) Homo sapiens 0-5 10037139-2 1999 GST fusion proteins with the calcyclin binding site of annexin XI-A, GST-AXI 34-62 and GST-AXI 49-77 bound to calcyclin-Sepharose Ca2+-dependently. Sepharose 120-129 S100 calcium binding protein A6 Homo sapiens 29-38 10037139-2 1999 GST fusion proteins with the calcyclin binding site of annexin XI-A, GST-AXI 34-62 and GST-AXI 49-77 bound to calcyclin-Sepharose Ca2+-dependently. Sepharose 120-129 S100 calcium binding protein A6 Homo sapiens 110-119 9895287-8 1999 Immunoaffinity chromatography on an anti-(apoA-I)-Sepharose column revealed that 10-20% of the apoA-II resided in particles that also contained apoA-I. Sepharose 50-59 apolipoprotein A2 Homo sapiens 95-102 9895287-8 1999 Immunoaffinity chromatography on an anti-(apoA-I)-Sepharose column revealed that 10-20% of the apoA-II resided in particles that also contained apoA-I. Sepharose 50-59 apolipoprotein A1 Homo sapiens 42-48 9895287-8 1999 Immunoaffinity chromatography on an anti-(apoA-I)-Sepharose column revealed that 10-20% of the apoA-II resided in particles that also contained apoA-I. Sepharose 50-59 apolipoprotein A1 Homo sapiens 95-101 9916029-3 1999 Single-headed myosin, which consists of a full length myosin heavy chain and a tagged tail, was isolated on the basis of the affinities for Nickel agarose and actin. Sepharose 147-154 myosin heavy chain 14 Homo sapiens 14-20 10205671-0 1999 Purification of cytochrome P-450 from pig testis by aniline-sepharose 4B and isoelectric focusing. Sepharose 60-69 cytochrome P450 family 2 subfamily D member 6 Sus scrofa 16-32 10400721-6 1999 Presence of PE2 was associated with an increase in HS-dependent attachment to cells and efficient attachment to heparin-agarose beads, presumably because the furin recognition site for PE2 cleavage also represents a candidate HS binding sequence. Sepharose 120-127 ETS2 repressor factor Homo sapiens 12-15 10477852-4 1999 After incubation of DRGs or PC 12 cells with YIGSR peptide or integrin beta1 antibody respectively, the neurite outgrowth promoting effects in LN-modified agarose gels were significantly decreased or abolished. Sepharose 155-162 integrin subunit beta 1 Rattus norvegicus 62-76 10477852-5 1999 These results indicate that DRG/PC 12 cell neurite outgrowth promoting effect of LN-modified agarose gels involves receptors for YIGSR/integrin beta1 subunits respectively. Sepharose 93-100 integrin subunit beta 1 Rattus norvegicus 135-149 10413105-5 1999 Affinity chromatography of a sized mixture of heparin oligosaccharides, having a degree of polymerization (dp) of > 14 saccharide units, on HB-GAM-Sepharose demonstrated that oligosaccharides having more than 18 saccharide residues showed the tightest interaction. Sepharose 150-159 pleiotrophin Homo sapiens 143-149 10429674-7 1999 The results from TCS-Sepharose pull-down and TCS and chemokine receptor coimmunoprecipitation and cross-linking experiments demonstrated association of TCS with CCR5. Sepharose 21-30 C-C motif chemokine receptor 5 Homo sapiens 161-165 10354208-4 1999 Alleles of an insertion/deletion polymorphism of the ACE gene were determined by one-stage polymerase chain reaction and visualized on agarose gels. Sepharose 135-142 angiotensin I converting enzyme Homo sapiens 53-56 10354214-3 1999 METHODS AND RESULTS: Using native agarose electrophoresis, we demonstrated that alpha2M binds [125I]-TGF-beta1 within minutes. Sepharose 34-41 alpha-2-macroglobulin Rattus norvegicus 80-87 10354214-3 1999 METHODS AND RESULTS: Using native agarose electrophoresis, we demonstrated that alpha2M binds [125I]-TGF-beta1 within minutes. Sepharose 34-41 transforming growth factor, beta 1 Rattus norvegicus 101-110 10470139-5 1999 Purification of the enzymatic activities using benzamidine sepharose yields a 50 kD and a 70 kD band of which the 50 kD band has fibronectin degrading capacity. Sepharose 59-68 fibronectin 1 Homo sapiens 129-140 10369126-3 1999 The binding of galectin-1 to CD45 was detected by affinity chromatography of NP 40 solubilized Jurkat T cell membranes on galectin-1 agarose followed by immunoblotting of the galectin-1 agarose bound fraction applying monoclonal antibodies to CD45 isoforms. Sepharose 133-140 galectin 1 Homo sapiens 15-25 10369126-3 1999 The binding of galectin-1 to CD45 was detected by affinity chromatography of NP 40 solubilized Jurkat T cell membranes on galectin-1 agarose followed by immunoblotting of the galectin-1 agarose bound fraction applying monoclonal antibodies to CD45 isoforms. Sepharose 133-140 protein tyrosine phosphatase receptor type C Homo sapiens 29-33 10369126-3 1999 The binding of galectin-1 to CD45 was detected by affinity chromatography of NP 40 solubilized Jurkat T cell membranes on galectin-1 agarose followed by immunoblotting of the galectin-1 agarose bound fraction applying monoclonal antibodies to CD45 isoforms. Sepharose 186-193 galectin 1 Homo sapiens 15-25 10369126-3 1999 The binding of galectin-1 to CD45 was detected by affinity chromatography of NP 40 solubilized Jurkat T cell membranes on galectin-1 agarose followed by immunoblotting of the galectin-1 agarose bound fraction applying monoclonal antibodies to CD45 isoforms. Sepharose 186-193 protein tyrosine phosphatase receptor type C Homo sapiens 29-33 10369126-4 1999 The PTPase activity of the galectin-1 agarose binding membrane fraction could be inhibited by sodium orthovanadate. Sepharose 38-45 galectin 1 Homo sapiens 27-37 10209280-1 1999 Cathepsin D was purified to homogeneity from the liver of Antarctic icefish by anion-exchange chromatography followed by affinity chromatography on concanavalin-A Sepharose. Sepharose 163-172 cathepsin D Homo sapiens 0-11 10092663-4 1999 Similarly, full-length [35S]mSec7-1/cytohesin was specifically adsorbed to glutathione-Sepharose loaded with glutathione S-transferase (GST)-ARP-Q79L, GST-ARP, or GST-ARP-T31N, the latter exhibiting the lowest binding affinity. Sepharose 87-96 cytohesin 1 Mus musculus 28-35 10092663-4 1999 Similarly, full-length [35S]mSec7-1/cytohesin was specifically adsorbed to glutathione-Sepharose loaded with glutathione S-transferase (GST)-ARP-Q79L, GST-ARP, or GST-ARP-T31N, the latter exhibiting the lowest binding affinity. Sepharose 87-96 glutathione S-transferase kappa 1 Homo sapiens 109-134 11593542-2 1999 METHODS: Rat anti-mouse antibodies coupled sepharose-4B beads were used to purify mouse H-2 antigens by affinity chromatography. Sepharose 43-52 histocompatibility-2, MHC Mus musculus 88-91 10103041-3 1999 The protein, designated Pen c 1, was purified by sequential DEAE-Sepharose and carboxymethyl (CM)-Sepharose chromatographies. Sepharose 65-74 proprotein convertase subtilisin/kexin type 1 inhibitor Homo sapiens 24-27 10103041-3 1999 The protein, designated Pen c 1, was purified by sequential DEAE-Sepharose and carboxymethyl (CM)-Sepharose chromatographies. Sepharose 65-74 heterogeneous nuclear ribonucleoprotein C Homo sapiens 28-31 10363718-4 1999 We demonstrate that two linear peptides (aa 124-138 and 130-143) and a cyclic one (aa 121-138) specifically bind to CD4-sepharose affinity columns. Sepharose 120-129 CD4 antigen Mus musculus 116-119 10402763-1 1999 We describe the separation of an active glutamate dehydrogenase [GDH (NADP+)] enzyme from the plasma of patients with P. falciparum infection using columns of sepharose anti-GDH (NADP+) of Proteus spp. Sepharose 159-168 glutamate dehydrogenase 1 Homo sapiens 65-68 10191282-2 1999 PLA2-treated Lp[a] had a decreased molecular weight, increased density, and greater electronegativity on agarose gels. Sepharose 105-112 phospholipase A2 group IB Homo sapiens 0-4 10191282-2 1999 PLA2-treated Lp[a] had a decreased molecular weight, increased density, and greater electronegativity on agarose gels. Sepharose 105-112 lipoprotein(a) Homo sapiens 13-17 11938994-5 1999 The lymphocytes treated with DON and AFG1 showed characteristic "ladder" pattern in agarose gel electrophoresis. Sepharose 84-91 AFG1 like ATPase Homo sapiens 37-41 10079081-17 1999 An SDK activity separated and present in "fraction 42" from Q-Sepharose chromatography specifically phosphorylated GRP105 (or GRP94) and HSP68 but did not phosphorylate PDI or 14-3-3. Sepharose 62-71 heat shock protein 90, beta (Grp94), member 1 Mus musculus 126-131 10079081-17 1999 An SDK activity separated and present in "fraction 42" from Q-Sepharose chromatography specifically phosphorylated GRP105 (or GRP94) and HSP68 but did not phosphorylate PDI or 14-3-3. Sepharose 62-71 heat shock protein 1B Mus musculus 137-142 10211407-4 1999 Among several methods for concentrating A beta from biological materials, immunoadsorption to Sepharose-bound antibodies was the most efficient. Sepharose 94-103 amyloid beta precursor protein Homo sapiens 40-46 10535696-4 1999 ACE was immobilized onto epoxy Sepharose as well as Affi-Gel 15. Sepharose 31-40 angiotensin I converting enzyme Bos taurus 0-3 10037172-6 1999 JNK was first extracted from the tumor lysates by incubation over a Sepharose-bound c-Jun(1-89) fusion protein, and its activity was then measured by chemiluminescent Western blot by detection of the phosphorylated product using a phospho-Jun(Ser-63)-specific primary antibody. Sepharose 68-77 mitogen-activated protein kinase 8 Homo sapiens 0-3 10074901-6 1999 Under the third condition (0.5% FCS plus 10 ng/ml basic fibroblast growth factor), astrocytes gained the ability to form colonies in soft agarose and had abnormal chromosome numbers similar to cells in the first two conditions but did not form tumors in nude mice or overexpress glioma-associated genes. Sepharose 138-145 fibroblast growth factor 2 Mus musculus 50-80 10190043-1 1999 Butyrylcholinesterase (BChE, EC 3.1.1.8) has been purified about 6600-fold from human serum with a procedure including ammonium sulfate fractionation (55-70%) with acid step at pH 4.5 and procainamide-Sepharose 4B affinity chromatography. Sepharose 201-210 cholinesterase Ovis aries 0-21 10190043-1 1999 Butyrylcholinesterase (BChE, EC 3.1.1.8) has been purified about 6600-fold from human serum with a procedure including ammonium sulfate fractionation (55-70%) with acid step at pH 4.5 and procainamide-Sepharose 4B affinity chromatography. Sepharose 201-210 cholinesterase Ovis aries 23-27 10197448-5 1999 Hsc70 is among the proteins coimmunoprecipitated with stathmin, and it is the main protein retained specifically on stathmin-Sepharose beads identified by one- and two-dimensional electrophoresis and immunoblots. Sepharose 125-134 heat shock protein family A (Hsp70) member 8 Homo sapiens 0-5 10197448-5 1999 Hsc70 is among the proteins coimmunoprecipitated with stathmin, and it is the main protein retained specifically on stathmin-Sepharose beads identified by one- and two-dimensional electrophoresis and immunoblots. Sepharose 125-134 stathmin 1 Homo sapiens 116-124 10197448-6 1999 Bovine serum albumin (BSA)-Sepharose did not bind Hsc70, and anti-stathmin antisera specifically inhibited the interaction of Hsc70 with stathmin-Sepharose. Sepharose 146-155 stathmin 1 Homo sapiens 66-74 10197448-6 1999 Bovine serum albumin (BSA)-Sepharose did not bind Hsc70, and anti-stathmin antisera specifically inhibited the interaction of Hsc70 with stathmin-Sepharose. Sepharose 146-155 heat shock protein family A (Hsp70) member 8 Homo sapiens 126-131 10197448-6 1999 Bovine serum albumin (BSA)-Sepharose did not bind Hsc70, and anti-stathmin antisera specifically inhibited the interaction of Hsc70 with stathmin-Sepharose. Sepharose 146-155 stathmin 1 Homo sapiens 137-145 9916061-11 1999 Subjecting the cell surface extract to affinity chromatography on an agarose-transferrin column revealed that it contained a protein having an estimated molecular mass of 37 kDa and possessing transferrin-binding activity. Sepharose 69-76 transferrin Homo sapiens 77-88 9916061-11 1999 Subjecting the cell surface extract to affinity chromatography on an agarose-transferrin column revealed that it contained a protein having an estimated molecular mass of 37 kDa and possessing transferrin-binding activity. Sepharose 69-76 transferrin Homo sapiens 193-204 10216966-9 1999 NADH-cytochrome b5 reductase was also partially purified from the same source, detergent solubilized sheep liver microsomes, by using two successive DEAE-cellulose, and 5"-ADP-agarose affinity column chromatographies. Sepharose 176-183 cytochrome b5 Ovis aries 5-18 9924193-5 1999 Streptavidin-agarose-immobilized TSHR-Xa-BIO was labeled with 125I using the chloramine T oxidation procedure and specifically eluted from the solid phase after cleavage with protease Xa. Sepharose 13-20 thyroid stimulating hormone receptor Homo sapiens 33-44 9925666-5 1999 The electrophoretic mobility of the recombinant apo[a] isoforms expressed by these cells in a hollow-fiber bioreactor was determined after reduction by SDS-gel (agarose, acrylamide or a mixture of both) electrophoresis and immunoblotting using an antibody specific for human apo[a]. Sepharose 161-168 aminopeptidase O (putative) Homo sapiens 48-51 9989767-4 1999 Total IgA1 in serum samples from patients with IgAN or from healthy controls was isolated with a jacalin-agarose column as jacalin-bound protein (JBP). Sepharose 105-112 immunoglobulin heavy constant alpha 1 Homo sapiens 6-10 10081758-5 1999 The recombinant IL-2, IL-6 and IFN-gamma could be obtained by the batch method using Glutathione Sepharose 4B and Factor Xa digestion, which may be useful for preparation of antisera as antigens and functional studies. Sepharose 97-106 interleukin 2 Bos taurus 16-20 10081758-5 1999 The recombinant IL-2, IL-6 and IFN-gamma could be obtained by the batch method using Glutathione Sepharose 4B and Factor Xa digestion, which may be useful for preparation of antisera as antigens and functional studies. Sepharose 97-106 interferon beta-2 Bos taurus 22-26 10081758-5 1999 The recombinant IL-2, IL-6 and IFN-gamma could be obtained by the batch method using Glutathione Sepharose 4B and Factor Xa digestion, which may be useful for preparation of antisera as antigens and functional studies. Sepharose 97-106 interferon gamma Bos taurus 31-40 9891085-6 1999 Biochemical evidence for an interaction was provided by the copurification on immunoglobulin G-Sepharose of Nip7p with protein A-tagged Rrp43p and Nop8p. Sepharose 95-104 ribosome biosynthesis protein NIP7 Saccharomyces cerevisiae S288C 108-113 9891085-6 1999 Biochemical evidence for an interaction was provided by the copurification on immunoglobulin G-Sepharose of Nip7p with protein A-tagged Rrp43p and Nop8p. Sepharose 95-104 exosome non-catalytic core subunit RRP43 Saccharomyces cerevisiae S288C 136-142 9891085-6 1999 Biochemical evidence for an interaction was provided by the copurification on immunoglobulin G-Sepharose of Nip7p with protein A-tagged Rrp43p and Nop8p. Sepharose 95-104 Nop8p Saccharomyces cerevisiae S288C 147-152 10052357-2 1999 Its three components-GroEL minichaperone (191-345), which can prevent protein aggregation; DsbA, which catalyzes the shuffling and oxidative formation of disulfide bonds; and peptidyl-prolyl isomerase-were immobilized on an agarose gel. Sepharose 224-231 heat shock protein family D (Hsp60) member 1 Homo sapiens 21-26 18726494-8 1999 The existence of the introduced plasmid pDC-TNF in recombinant cyanobacterial cells has been demonstrated by the results of the agarose electrophoresis with the extracted plasmid samples and Southern blotting with alpha-(32)P labeled hTNF cDNA probes, while the expression of the hTNF gene in Anabaena sp. Sepharose 128-135 tumor necrosis factor Homo sapiens 44-47 10082391-2 1999 The induced intracellular glutathione S-transferase (GST) fusion proteins of HBeAg-MV and HBeAg-SV were recovered and purified from bacterial lysates by affinity chromatography with glutathione-sepharose beads. Sepharose 194-203 glutathione S-transferase kappa 1 Homo sapiens 26-51 10082391-2 1999 The induced intracellular glutathione S-transferase (GST) fusion proteins of HBeAg-MV and HBeAg-SV were recovered and purified from bacterial lysates by affinity chromatography with glutathione-sepharose beads. Sepharose 194-203 glutathione S-transferase kappa 1 Homo sapiens 53-56 9882631-6 1999 The progress of topotecan- or anti-CD95-induced cell death was monitored by flow cytometry analysis and also by agarose electrophoresis of fragmented DNA. Sepharose 112-119 Fas cell surface death receptor Homo sapiens 35-39 9880564-13 1999 This CETP inhibitor activity was efficiently removed from the media by nickel-Sepharose, consistent with the 6-His tag incorporated into recombinant apoF. Sepharose 78-87 cholesteryl ester transfer protein Homo sapiens 5-9 9880564-13 1999 This CETP inhibitor activity was efficiently removed from the media by nickel-Sepharose, consistent with the 6-His tag incorporated into recombinant apoF. Sepharose 78-87 apolipoprotein F Homo sapiens 149-153 10048202-7 1999 An important genotyping application is also shown by characterizing the polymorphic region (2 X or 4 X 48 base pair repeats) of the dopamine D4 receptor gene (D4DR, exon III region) for ten individuals, using PCR technology with Cy5-labeled primers and ultra-thin-layer agarose gel electrophoresis. Sepharose 270-277 dopamine receptor D4 Homo sapiens 132-152 10048202-7 1999 An important genotyping application is also shown by characterizing the polymorphic region (2 X or 4 X 48 base pair repeats) of the dopamine D4 receptor gene (D4DR, exon III region) for ten individuals, using PCR technology with Cy5-labeled primers and ultra-thin-layer agarose gel electrophoresis. Sepharose 270-277 dopamine receptor D4 Homo sapiens 159-163 9990301-3 1999 By applying agarose gel differential display to these populations we cloned a cDNA encoding a novel 268 amino acid protein (3X11A). Sepharose 12-19 phosphoethanolamine/phosphocholine phosphatase Gallus gallus 124-129 9873003-5 1999 The existence of protein kinase-PP2A complexes in rat brain soluble extracts was further substantiated by the following results: 1) independent immunoprecipitation of the kinases revealed that PP2A co-precipitated with p70 S6 kinase and the two PAK isoforms; 2) glutathione S-transferase fusion proteins of p70 S6 kinase and PAK3 each isolated PP2A; and 3) PAK3 and p70 S6 kinase bound to microcystin-Sepharose (an affinity resin for PP2A-PP1). Sepharose 401-410 p21 (RAC1) activated kinase 1 Rattus norvegicus 245-248 9923612-2 1999 The human APLP2 ectodomain (sAPLP2) was expressed in the yeast Pichia pastoris and the recombinant sAPLP2 was purified from the culture medium in a single step by metal-chelating Sepharose chromatography. Sepharose 179-188 amyloid beta precursor like protein 2 Homo sapiens 10-15 10824864-9 1999 Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit a skeletal muscle actin (Mr 43,000) and isoelectric point equal to 5.3. Sepharose 79-86 actin Oryctolagus cuniculus 0-5 10824864-9 1999 Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit a skeletal muscle actin (Mr 43,000) and isoelectric point equal to 5.3. Sepharose 79-86 deoxyribonuclease-1 Oryctolagus cuniculus 62-69 10824864-9 1999 Actin isolated from the cytosol by affinity chromatography on DNase I bound to agarose shows the presence of only one protein spot on 2D gel electrophoresis corresponding to the mobility of the rabbit a skeletal muscle actin (Mr 43,000) and isoelectric point equal to 5.3. Sepharose 79-86 actin Oryctolagus cuniculus 219-224 9888876-3 1999 Three natriuretic peptides (atrial natriuretic peptide, brain natriuretic peptide, and C-type natriuretic peptide) induced endothelial apoptosis as demonstrated by nucleosomal laddering on agarose gel electrophoresis and by the terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling method. Sepharose 189-196 natriuretic peptide C Homo sapiens 87-113 9854036-4 1999 Mouse GSTT1-1 was expressed in Escherichia coli as an N-terminal 6x histidine-tagged protein and purified using immobilized-metal affinity chromatography on nickel-agarose. Sepharose 164-171 glutathione S-transferase, theta 1 Mus musculus 6-13 9989668-1 1999 Deoxycytidine kinase from Ehrlich carcinoma cells was purified 10400-fold by ammonium sulfate fractionation and affinity chromatography using Sepharose 4B coupled to 2"-C-cyano-2"-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine , with a yield of 45%. Sepharose 142-154 deoxycytidine kinase Mus musculus 0-20 10735644-0 1999 Criteria of evaluation and of interpretation of Sepharose drug IgE-RIA to anaesthetic drugs. Sepharose 48-57 immunoglobulin heavy constant epsilon Homo sapiens 63-66 10643638-3 1999 Here, we demonstrate the binding of human matrix metalloproteinase 1 to a thiophilic resin (mercaptoethylquinazolinedione derivatized agarose) and take advantage of this thiophilic interaction for the purification of organomercurial activated matrix metalloproteinase 1 from the supernatant of a thyroid carcinoma cell line in connection with the simultaneous removal of the activator. Sepharose 134-141 matrix metallopeptidase 1 Homo sapiens 42-68 10643638-3 1999 Here, we demonstrate the binding of human matrix metalloproteinase 1 to a thiophilic resin (mercaptoethylquinazolinedione derivatized agarose) and take advantage of this thiophilic interaction for the purification of organomercurial activated matrix metalloproteinase 1 from the supernatant of a thyroid carcinoma cell line in connection with the simultaneous removal of the activator. Sepharose 134-141 matrix metallopeptidase 1 Homo sapiens 243-269 10051128-12 1999 Agarose gel analysis of the PCR products followed by ethidium bromide fluorescence or CSPD chemiluminescence detection revealed an almost exclusive expression of the NR1 splice variants lacking exon (E) 5 and E22. Sepharose 0-7 glutamate ionotropic receptor NMDA type subunit 1 Rattus norvegicus 166-169 10322636-6 1999 Applied locally on agarose beads, neurturin induces supernumerary ureteric buds to emerge from the wolffian duct and causes nearby collecting duct branches to distend to an abnormally large diameter. Sepharose 19-26 neurturin Homo sapiens 34-43 10023114-4 1999 ACE (I/D) genotype was determined using polymerase chain reaction and 3% agarose gel electrophoresis. Sepharose 73-80 angiotensin I converting enzyme Homo sapiens 0-3 10612487-6 1999 Biotinylated TSHR-BIO-6HIS was immobilized on Ni-NTA agarose and selectively labeled with a biotin binding protein-- 125I-neutravidin. Sepharose 53-60 thyroid stimulating hormone receptor Homo sapiens 13-17 10830921-4 1999 The patients" multimeric vWF pattern was analyzed by sodium dodecylsulfate (SDS)-agarose-acrylamide electrophoresis, Western blot, and densitometric analysis. Sepharose 81-88 von Willebrand factor Homo sapiens 25-28 10612487-7 1999 The 125I-neutravidin labeled TSHR-BIO-6HIS, freed of the excess of nonbound radioactivity, was eluted from Ni-NTA agarose and used for the detection of pathological autoantibodies in 50 Graves" disease, 10 Hashimoto"s disease, 10 insulin-dependent diabetes mellitus and 50 normal sera. Sepharose 114-121 thyroid stimulating hormone receptor Homo sapiens 29-33 9950264-2 1999 NSF-60 cells proliferate in response to interleukin-3 (IL-3) and undergo apoptosis when deprived of exogenous IL-3, as demonstrated by the appearance of characteristic DNA "ladders" following agarose gel electrophoresis. Sepharose 192-199 N-ethylmaleimide sensitive fusion protein Mus musculus 0-3 9950264-2 1999 NSF-60 cells proliferate in response to interleukin-3 (IL-3) and undergo apoptosis when deprived of exogenous IL-3, as demonstrated by the appearance of characteristic DNA "ladders" following agarose gel electrophoresis. Sepharose 192-199 interleukin 3 Mus musculus 110-114 9925962-5 1999 We also examined whether cultured eosinophils degranulated eosinophil-derived neurotoxin (EDN) induced by secretory immunoglobulin A conjugated to sepharose beads (sIgA-beads) and responded to eotaxin. Sepharose 147-156 ribonuclease A family member 2 Homo sapiens 59-88 9925962-5 1999 We also examined whether cultured eosinophils degranulated eosinophil-derived neurotoxin (EDN) induced by secretory immunoglobulin A conjugated to sepharose beads (sIgA-beads) and responded to eotaxin. Sepharose 147-156 ribonuclease A family member 2 Homo sapiens 90-93 9927147-4 1999 Bile hepatocyte growth factor was purified on a heparin-Sepharose column and subjected to Western blotting. Sepharose 56-65 hepatocyte growth factor Homo sapiens 5-29 9920842-8 1999 HO-1 expressed mRNA for apoptosis-inducing mediators, including perforin, granzyme B, Fas ligand, TNF-alpha, and lymphotoxin; however, no DNA fragmentation was detected in target cells incubated with HO-1 by 5-[125I]Iodo-2"-deoxyuridine release assay and agarose gel electrophoresis of DNA. Sepharose 255-262 heme oxygenase 1 Homo sapiens 0-4 10094397-6 1999 We had also observed that rat liver membrane glycoproteins obtained through chromatography on wheat-germ lectin-Sepharose contain CK2 activity which copurifies with grp94/endoplasmin. Sepharose 112-121 casein kinase 2 beta Rattus norvegicus 130-133 9886097-8 1999 The glycosylated rds/peripherin..rom1 complex bound to concanavalin A-Sepharose, suggesting that the glycan is not directly involved in the interaction between these proteins. Sepharose 70-79 peripherin 2 Mus musculus 17-31 9886097-8 1999 The glycosylated rds/peripherin..rom1 complex bound to concanavalin A-Sepharose, suggesting that the glycan is not directly involved in the interaction between these proteins. Sepharose 70-79 rod outer segment membrane protein 1 Mus musculus 33-37 10071932-3 1999 Similar apparent KD values for the complex GroEL x GAPDH were obtained in both cases (0.04 and 0.03 microM, respectively), the stoichiometry being 1.0 mol chaperonin per GAPDH subunit in the system with the immobilized GroEL and 0.2 mol chaperonin per Sepharose-bound GAPDH monomer. Sepharose 252-261 GroEL Escherichia coli 43-48 10071932-5 1999 Incubation of the Sepharose-bound catalytically active tetrameric and dimeric GAPDH forms with the protein fraction of a wild-type E. coli cell extract resulted in the binding of GroEL to the dimer and no interaction with the tetrameric form. Sepharose 18-27 GroEL Escherichia coli 179-184 10210284-3 1999 Following incubation of the cells with Mic-1 (10-100 nM) or Mic-3 (10-100 nM), internucleosomal DNA fragmentation in apoptotic cells was detected by agarose gel electrophoresis and the diphenylamine (DPA) assay; the presence of hypodiploid nuclei assessed by propidium iodide (PI) staining; and the percentages of apoptotic and necrotic cells quantified by morphological observation and fluorescein labeled annexin-V binding. Sepharose 149-156 regulator of MON1-CCZ1 Mus musculus 39-44 10094397-9 1999 Calreticulin and grp94/endoplasmin could be partially resolved from CK2 by chromatography on heparin-agarose and almost completely on ConA-Sepharose. Sepharose 139-148 calreticulin Rattus norvegicus 0-12 10094397-9 1999 Calreticulin and grp94/endoplasmin could be partially resolved from CK2 by chromatography on heparin-agarose and almost completely on ConA-Sepharose. Sepharose 139-148 heat shock protein 90 beta family member 1 Rattus norvegicus 17-22 10094397-9 1999 Calreticulin and grp94/endoplasmin could be partially resolved from CK2 by chromatography on heparin-agarose and almost completely on ConA-Sepharose. Sepharose 139-148 heat shock protein 90 beta family member 1 Rattus norvegicus 23-34 10094397-9 1999 Calreticulin and grp94/endoplasmin could be partially resolved from CK2 by chromatography on heparin-agarose and almost completely on ConA-Sepharose. Sepharose 101-108 calreticulin Rattus norvegicus 0-12 10094397-9 1999 Calreticulin and grp94/endoplasmin could be partially resolved from CK2 by chromatography on heparin-agarose and almost completely on ConA-Sepharose. Sepharose 101-108 heat shock protein 90 beta family member 1 Rattus norvegicus 17-22 10094397-9 1999 Calreticulin and grp94/endoplasmin could be partially resolved from CK2 by chromatography on heparin-agarose and almost completely on ConA-Sepharose. Sepharose 101-108 heat shock protein 90 beta family member 1 Rattus norvegicus 23-34 10094397-9 1999 Calreticulin and grp94/endoplasmin could be partially resolved from CK2 by chromatography on heparin-agarose and almost completely on ConA-Sepharose. Sepharose 101-108 casein kinase 2 beta Rattus norvegicus 68-71 10092156-1 1999 Two proteins of 17 and 24 kDa, respectively, which were immunologically related to bikunin, were purified from urine of healthy men, using in the last step a trypsin CNBr-sepharose affinity column. Sepharose 171-180 alpha-1-microglobulin/bikunin precursor Homo sapiens 83-90 10643050-7 1999 NF1-rich nuclear protein fraction was purified from the rat liver nuclear extract by DEAE-cellulose and heparin-sepharose chromatography. Sepharose 112-121 neurofibromin 1 Rattus norvegicus 0-3 9922179-2 1998 In this study, we have investigated the possibility that protein phosphatase-1 (PP1) is targeted to the junctional sarcoplasmic reticulum by the direct isolation of PP1-binding proteins on PP1-Sepharose affinity columns. Sepharose 193-202 neuropeptide Y receptor Y4 Homo sapiens 57-78 9922179-2 1998 In this study, we have investigated the possibility that protein phosphatase-1 (PP1) is targeted to the junctional sarcoplasmic reticulum by the direct isolation of PP1-binding proteins on PP1-Sepharose affinity columns. Sepharose 193-202 neuropeptide Y receptor Y4 Homo sapiens 80-83 9922179-2 1998 In this study, we have investigated the possibility that protein phosphatase-1 (PP1) is targeted to the junctional sarcoplasmic reticulum by the direct isolation of PP1-binding proteins on PP1-Sepharose affinity columns. Sepharose 193-202 neuropeptide Y receptor Y4 Homo sapiens 165-168 9922179-2 1998 In this study, we have investigated the possibility that protein phosphatase-1 (PP1) is targeted to the junctional sarcoplasmic reticulum by the direct isolation of PP1-binding proteins on PP1-Sepharose affinity columns. Sepharose 193-202 neuropeptide Y receptor Y4 Homo sapiens 165-168 9922179-5 1998 The direct binding of PP1 to the ryanodine receptor was supported by the finding that tryptic fragments of the receptor were retained on PP1-Sepharose. Sepharose 141-150 neuropeptide Y receptor Y4 Homo sapiens 22-25 9922179-5 1998 The direct binding of PP1 to the ryanodine receptor was supported by the finding that tryptic fragments of the receptor were retained on PP1-Sepharose. Sepharose 141-150 neuropeptide Y receptor Y4 Homo sapiens 137-140 9841882-5 1998 We first provide evidence that the CyPB binding to heparin-Sepharose is prevented by soluble sulphated glycosaminoglycans (GAG), raising the interesting possibility that such interactions may occur on the T-cell surface. Sepharose 59-68 peptidylprolyl isomerase B Homo sapiens 35-39 9857175-7 1998 These observations were corroborated by subjecting a peroxisomal matrix protein fraction to affinity chromatography on Sepharose-immobilized pre-nsL-TP. Sepharose 119-128 sterol carrier protein 2 Homo sapiens 145-151 9837878-1 1998 The 80/40-kDa CD98 protein complex was purified using an anti-CD98 heavy chain monoclonal antibody coupled to Sepharose beads. Sepharose 110-119 solute carrier family 7 member 5 Homo sapiens 14-18 10206734-1 1998 A porcine liver 40 kDa protein designated SBP40 isolated by affinity chromatography with agarose-linked spermine was identified as a porcine cytokeratin 18 on the basis of partial amino acid sequences of peptides derived by lysylendopeptidase digestion and by its reactivity with two commercially available preparations of monoclonal antibody. Sepharose 89-96 keratin 18 Mus musculus 141-155 9853960-8 1998 Similar to mouse odontogenic epithelium, agarose beads soaked in recombinant BMP-4 induced a translucent zone, cellular proliferation, and expression of Msx-1, Msx-2, and Bmp-4 in chick mandibular mesenchyme after 24 hours. Sepharose 41-48 bone morphogenetic protein 4 Mus musculus 77-82 10743701-3 1999 This was carried out following isolation of PLAP from biological samples using CNBr Sepharose-conjugated ATC2 beads. Sepharose 84-93 alkaline phosphatase, placental Homo sapiens 44-48 10743701-3 1999 This was carried out following isolation of PLAP from biological samples using CNBr Sepharose-conjugated ATC2 beads. Sepharose 84-93 corin, serine peptidase Homo sapiens 105-109 9874241-4 1998 The fusion protein was bound to glutathione-agarose to form the cyclophilin-D affinity matrix. Sepharose 44-51 peptidylprolyl isomerase D Rattus norvegicus 64-77 9990335-10 1998 The following differences were observed in KCS secreted mucins compared to normal samples: an increase in the proportion of mucin with low buoyant density; a decrease in mannose content detected with Concanavalin A lectin; an increase in N-acetylglucosamine structures detected with Lycopersicon esculentum lectin; increased migration and lack of evidence for distinct subunit structure on agarose gels. Sepharose 390-397 mucin Canis lupus familiaris 56-61 9856832-6 1998 HDMEC membranes extracted with Triton X114 were incubated with gelatin-sepharose purified MMP-2 and MMP-9 to show activation of proenzymes. Sepharose 71-80 matrix metallopeptidase 2 Homo sapiens 90-95 9864293-4 1998 Moreover, nNOS bound to a HMN-1180-coupled Sepharose column, but eNOS and iNOS did not. Sepharose 43-52 nitric oxide synthase 1 Homo sapiens 10-14 9836747-4 1998 The immunoresponse to the antibodies correlated with the enzymatic activity profile of the RdRP after chromatography on Q-, poly(A)-, and poly(U)-Sepharose, hydroxyapatite, and Sephadex G-200 columns. Sepharose 146-155 RNA-directed RNA polymerase Solanum lycopersicum 91-95 9847114-5 1998 Recently, purified mammalian p23 preadsorbed with JJ3 antibody-protein A-Sepharose pellets was used to isolate a mammalian p23-wheat hsp90 heterocomplex from wheat germ lysate (J.K. Owens-Grillo, L.F. Stancato, K. Hoffmann, W.B. Sepharose 73-82 prostaglandin E synthase 3 Homo sapiens 29-32 9847114-5 1998 Recently, purified mammalian p23 preadsorbed with JJ3 antibody-protein A-Sepharose pellets was used to isolate a mammalian p23-wheat hsp90 heterocomplex from wheat germ lysate (J.K. Owens-Grillo, L.F. Stancato, K. Hoffmann, W.B. Sepharose 73-82 prostaglandin E synthase 3 Homo sapiens 123-126 9847114-5 1998 Recently, purified mammalian p23 preadsorbed with JJ3 antibody-protein A-Sepharose pellets was used to isolate a mammalian p23-wheat hsp90 heterocomplex from wheat germ lysate (J.K. Owens-Grillo, L.F. Stancato, K. Hoffmann, W.B. Sepharose 73-82 heat shock protein 83 Triticum aestivum 133-138 9839674-1 1998 Bothroalternin (MW 27 kDa), a new member of the family of C-type lectins is a thrombin inhibitor which was purified from pooled B. alternatus venom by affinity chromatography on PPACK-thrombin-Sepharose, followed by size exclusion and reverse-phase on HPLC columns. Sepharose 193-202 prothrombin Oryctolagus cuniculus 78-86 9839674-1 1998 Bothroalternin (MW 27 kDa), a new member of the family of C-type lectins is a thrombin inhibitor which was purified from pooled B. alternatus venom by affinity chromatography on PPACK-thrombin-Sepharose, followed by size exclusion and reverse-phase on HPLC columns. Sepharose 193-202 prothrombin Oryctolagus cuniculus 184-192 9853960-8 1998 Similar to mouse odontogenic epithelium, agarose beads soaked in recombinant BMP-4 induced a translucent zone, cellular proliferation, and expression of Msx-1, Msx-2, and Bmp-4 in chick mandibular mesenchyme after 24 hours. Sepharose 41-48 msh homeobox 1 Mus musculus 153-158 9853960-8 1998 Similar to mouse odontogenic epithelium, agarose beads soaked in recombinant BMP-4 induced a translucent zone, cellular proliferation, and expression of Msx-1, Msx-2, and Bmp-4 in chick mandibular mesenchyme after 24 hours. Sepharose 41-48 msh homeobox 2 Mus musculus 160-165 9853960-8 1998 Similar to mouse odontogenic epithelium, agarose beads soaked in recombinant BMP-4 induced a translucent zone, cellular proliferation, and expression of Msx-1, Msx-2, and Bmp-4 in chick mandibular mesenchyme after 24 hours. Sepharose 41-48 bone morphogenetic protein 4 Mus musculus 171-176 9812994-7 1998 Heparin-Sepharose chromatography demonstrated that HL-LPLC1 and HL-LPLC2 eluted at 0.80 and 1.3 M NaCl, respectively, elution positions that corresponded to native HL and LPL. Sepharose 8-17 lipoprotein lipase Homo sapiens 54-57 9866676-2 1998 The stained zone diameters resulting from the hydrolysis of 0.1% (w/v) 90% esterified pectin in an agarose gel by diffused, commercial PME were log-linear over 4 orders of magnitude, with a minimum detection limit of 3.6 pkatals. Sepharose 99-106 pectinesterase/pectinesterase inhibitor U1 Solanum lycopersicum 135-138 9801304-8 1998 Recombinant as well as native CRY1 proteins from mouse and human cells showed a tight binding activity to DNA Sepharose, while CRY2 protein did not bind to DNA Sepharose at all under the same condition as CRY1. Sepharose 110-119 cryptochrome 1 (photolyase-like) Mus musculus 30-34 9792715-10 1998 The presence of 4 +/- 0.4 microM GST-DHPR II-III or 5 +/- 0.1 microM His-peptide-DHPR III-IV was required for half-maximal co-purification of 35S-labeled RyR1 Leu922-Asp1112 on glutathione-Sepharose or Ni2+-nitrilotriacetic acid. Sepharose 189-198 glutathione S-transferase kappa 1 Homo sapiens 33-36 9792715-10 1998 The presence of 4 +/- 0.4 microM GST-DHPR II-III or 5 +/- 0.1 microM His-peptide-DHPR III-IV was required for half-maximal co-purification of 35S-labeled RyR1 Leu922-Asp1112 on glutathione-Sepharose or Ni2+-nitrilotriacetic acid. Sepharose 189-198 quinoid dihydropteridine reductase Homo sapiens 37-41 9814975-6 1998 Domain-selective cell surface biotinylation followed by immunoblotting of streptavidin-agarose-fractionated biotinylated glycoproteins independently confirmed the polarized distribution of FLAG epitope-tagged hCTR-2 in the basolateral domain. Sepharose 87-94 solute carrier family 31 member 2 Homo sapiens 209-215 9875280-4 1998 Overexpression of HA-Bax in MN9D cells was shown to attenuate 6-OHDA-induced cell death as determined by the MTT reduction assay and agarose gel analysis for DNA fragmentation. Sepharose 133-140 BCL2-associated X protein Mus musculus 21-24 9839935-3 1998 The supernatant is applied to a maltosyl-agarose column and the bound SP-D is specifically eluted using MnCl2. Sepharose 41-48 surfactant protein D Homo sapiens 70-74 9811480-5 1998 Alginate/heparin-Sepharose microspheres and films were designed as drug carriers to control release the bFGF-SAP conjugate or bFGF alone in small doses. Sepharose 17-26 fibroblast growth factor 2 Bos taurus 104-108 9811480-5 1998 Alginate/heparin-Sepharose microspheres and films were designed as drug carriers to control release the bFGF-SAP conjugate or bFGF alone in small doses. Sepharose 17-26 fibroblast growth factor 2 Bos taurus 126-130 9817593-10 1998 Depletion assays using IGF-II-Sepharose showed that the mutant receptor had lower affinity for IGF-II than the wild-type receptor. Sepharose 30-39 insulin like growth factor 2 Homo sapiens 23-29 9817593-10 1998 Depletion assays using IGF-II-Sepharose showed that the mutant receptor had lower affinity for IGF-II than the wild-type receptor. Sepharose 30-39 insulin like growth factor 2 Homo sapiens 95-101 9832358-3 1998 Other serine proteases (C2, factor II, factor IX, trypsin, chymotrypsin, proteinase 3) also bound to Blue Sepharose but only those belonging to the trypsin family could be eluted with benzamidine. Sepharose 106-115 complement C2 Homo sapiens 24-48 9832358-3 1998 Other serine proteases (C2, factor II, factor IX, trypsin, chymotrypsin, proteinase 3) also bound to Blue Sepharose but only those belonging to the trypsin family could be eluted with benzamidine. Sepharose 106-115 proteinase 3 Homo sapiens 73-85 9853702-0 1998 Differential expression of double-band apolipoprotein(a) phenotypes in healthy Spanish subjects detected by SDS-agarose immunoblotting. Sepharose 112-119 lipoprotein(a) Homo sapiens 39-56 10211703-1 1998 P-selectin glycoprotein ligand-1, PSGL-1, a specific ligand for P-, E-, and L-selectin, was isolated from in vivo [3H]-glucosamine labeled HL-60 cells by a combination of wheat germ agglutinin and platelet P-selectin- or E-selectin receptor globulin-agarose chromatography. Sepharose 250-257 selectin P ligand Homo sapiens 34-40 9796912-4 1998 The CD23 molecule was in the form of a trimer in the soluble complex purified from plasma by affinity chromatography on anti-CD21 Sepharose. Sepharose 130-139 Fc epsilon receptor II Homo sapiens 4-8 9796912-4 1998 The CD23 molecule was in the form of a trimer in the soluble complex purified from plasma by affinity chromatography on anti-CD21 Sepharose. Sepharose 130-139 complement C3d receptor 2 Homo sapiens 125-129 9736462-9 1998 Regucalcin has been demonstrated to bind on calmodulin-agarose beads by analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 55-62 regucalcin Rattus norvegicus 0-10 9736462-9 1998 Regucalcin has been demonstrated to bind on calmodulin-agarose beads by analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 55-62 calmodulin 1 Rattus norvegicus 44-54 9737974-8 1998 HIP/L29 directly binds 125I-Hp in gel overlay assays and requires 0.75 M NaCl for elution from Hp-agarose. Sepharose 98-105 ribosomal protein L29 Homo sapiens 0-3 9737974-8 1998 HIP/L29 directly binds 125I-Hp in gel overlay assays and requires 0.75 M NaCl for elution from Hp-agarose. Sepharose 98-105 ribosomal protein L27A Mus musculus 4-7 9743356-6 1998 We propose that the molecular structure(s) on NK cells mediating the inhibitory effects HIV-1 Tat belong to L-type calcium channels, based on three lines of evidence: 1) binding of phenylalkylamine derivatives to these channels is cross-inhibited by Tat; 2) L-type calcium channels from NK cell lysates bind to Tat linked to Sepharose columns; 3) the inhibitory effect of HIV-1 Tat on NK cell function is prevented by the agonist of L-type calcium channels, Bay K 8644. Sepharose 325-334 Tat Human immunodeficiency virus 1 94-97 9743356-6 1998 We propose that the molecular structure(s) on NK cells mediating the inhibitory effects HIV-1 Tat belong to L-type calcium channels, based on three lines of evidence: 1) binding of phenylalkylamine derivatives to these channels is cross-inhibited by Tat; 2) L-type calcium channels from NK cell lysates bind to Tat linked to Sepharose columns; 3) the inhibitory effect of HIV-1 Tat on NK cell function is prevented by the agonist of L-type calcium channels, Bay K 8644. Sepharose 325-334 Tat Human immunodeficiency virus 1 250-253 9743356-6 1998 We propose that the molecular structure(s) on NK cells mediating the inhibitory effects HIV-1 Tat belong to L-type calcium channels, based on three lines of evidence: 1) binding of phenylalkylamine derivatives to these channels is cross-inhibited by Tat; 2) L-type calcium channels from NK cell lysates bind to Tat linked to Sepharose columns; 3) the inhibitory effect of HIV-1 Tat on NK cell function is prevented by the agonist of L-type calcium channels, Bay K 8644. Sepharose 325-334 Tat Human immunodeficiency virus 1 250-253 9743356-6 1998 We propose that the molecular structure(s) on NK cells mediating the inhibitory effects HIV-1 Tat belong to L-type calcium channels, based on three lines of evidence: 1) binding of phenylalkylamine derivatives to these channels is cross-inhibited by Tat; 2) L-type calcium channels from NK cell lysates bind to Tat linked to Sepharose columns; 3) the inhibitory effect of HIV-1 Tat on NK cell function is prevented by the agonist of L-type calcium channels, Bay K 8644. Sepharose 325-334 Tat Human immunodeficiency virus 1 250-253 9727035-5 1998 Rat skeletal muscle laminins partially purified by heparin-agarose affinity chromatography also bound alpha-dystroglycan without sensitivity to heparin. Sepharose 59-66 dystroglycan 1 Rattus norvegicus 102-120 9722586-5 1998 Nip1p can be isolated in an about 670-kDa complex containing polyhistidine-tagged Prt1p, a subunit of translation initiation factor 3, by binding to Ni2+-NTA-agarose beads in a manner completely dependent on the tagged form of Prt1p. Sepharose 158-165 translation initiation factor eIF3 core subunit c Saccharomyces cerevisiae S288C 0-5 9722586-5 1998 Nip1p can be isolated in an about 670-kDa complex containing polyhistidine-tagged Prt1p, a subunit of translation initiation factor 3, by binding to Ni2+-NTA-agarose beads in a manner completely dependent on the tagged form of Prt1p. Sepharose 158-165 translation initiation factor eIF3 core subunit b Saccharomyces cerevisiae S288C 82-87 9748646-2 1998 The four PLRP1s were identified using microsequencing methods after performing gel filtration on Ultrogel AcA-54 followed by chromatography on Heparin-Sepharose cation-exchanger. Sepharose 151-160 pancreatic lipase related protein 1 Homo sapiens 9-14 9831173-2 1998 Because IL-10 is a potent anti-inflammatory cytokine, we analyzed its in vitro effect on chemotaxis using an under agarose method. Sepharose 115-122 interleukin 10 Homo sapiens 8-13 9722667-6 1998 The proteolytic activity of PA-PGC was used for digesting the blocking reagent around the target antigen (in situ digestion method) or casein-clotting in the agarose plate containing skimmed milk (caseogram print method). Sepharose 158-165 progastricsin Homo sapiens 31-34 9696811-2 1998 The genetic interaction was confirmed by the specific coprecipitation of the NS1 protein from solution by a glutathione S-transferase-NS1-BP fusion protein and glutathione-Sepharose. Sepharose 172-181 influenza virus NS1A binding protein Homo sapiens 77-80 9725916-10 1998 We purified LAP-binding integrins from extracts of A549 cells using LAP bound to Sepharose. Sepharose 81-90 transforming growth factor beta 1 Homo sapiens 12-15 9725916-10 1998 We purified LAP-binding integrins from extracts of A549 cells using LAP bound to Sepharose. Sepharose 81-90 transforming growth factor beta 1 Homo sapiens 68-71 9731231-1 1998 Cartilage-specific functional matrix chondromodulin-I (ChM-I) is a 25 kDa glycoprotein purified from fetal bovine epiphyseal cartilage which stimulates the growth of rabbit chondrocytes and the colony formation of the cells in agarose. Sepharose 227-234 chondromodulin Bos taurus 37-53 9731231-1 1998 Cartilage-specific functional matrix chondromodulin-I (ChM-I) is a 25 kDa glycoprotein purified from fetal bovine epiphyseal cartilage which stimulates the growth of rabbit chondrocytes and the colony formation of the cells in agarose. Sepharose 227-234 chondromodulin Bos taurus 55-60 9705864-4 1998 When purified ASGPRs were bound to ligand-Sepharose, the CRD of RHL1, but not RHL2 or RHL3, was resistant to digestion with subtilisin. Sepharose 42-51 asialoglycoprotein receptor 1 Rattus norvegicus 64-68 9803314-5 1998 The S100A1 sequence identified contains a cluster of three hydrophobic residues (Phe-88, Phe-89 and Trp-90) at least one of which--as revealed by qualitative phenyl Sepharose binding and hydrophobic fluorescent probe spectroscopy--is exposed on the protein surface of Ca2+ bound S100A1. Sepharose 165-174 S100 calcium binding protein A1 Rattus norvegicus 4-10 10098613-3 1998 A test rig mounted on the stage of an inverted microscope was used to apply precise levels of compressive strain to individual cell-agarose constructs bathed in culture medium. Sepharose 132-139 dickkopf WNT signaling pathway inhibitor 3 Homo sapiens 7-10 9809796-3 1998 Insulin and IGF-I receptors were semipurified by affinity chromatography (WGA-agarose). Sepharose 78-85 insulin-like growth factor I Oncorhynchus mykiss 12-17 9820590-3 1998 An Alu element insertion in the ZFX intron results in a size difference in the amplicons which is resolved by agarose gel electrophoresis. Sepharose 110-117 zinc finger protein X-linked Homo sapiens 32-35 9809751-8 1998 CPX-2 is able to bind to Sepharose-Arg; this binding is blocked by 10 mM Arg. Sepharose 25-34 carboxypeptidase X 2 (M14 family) Mus musculus 0-5 9733854-6 1998 After partial purification using three different columns (DEAE-Sepharose, Econo S, and heparin-agarose), LRP was primarily associated with cdk2-cyclin E complexes, an enzyme which is necessary for G1-to-S-phase cell cycle progression. Sepharose 95-102 ribosomal protein SA Homo sapiens 105-108 9751364-6 1998 To determine the TaqI restriction fragment length polymorphism a 740 base pairs (bp) segment of the vitamin D receptor was amplified by PCR, digested with TaqI endonuclease and resolved on an agarose gel. Sepharose 192-199 vitamin D receptor Homo sapiens 100-118 9784842-2 1998 The purified HBp17 was digested by staphylococcus urcus V8 protease or chymotrypsin and the heparin-binding fragments were isolated by Heparin-Sepharose. Sepharose 143-152 fibroblast growth factor binding protein 1 Homo sapiens 13-18 9721171-8 1998 PHAS-I/eIF-4E association was measured as the amount of PHAS-I recovered after purification of translation factor eIF-4E by 7-methyl guanosine triphosphate-Sepharose. Sepharose 156-165 eukaryotic translation initiation factor 4E binding protein 1 Rattus norvegicus 0-6 9721171-8 1998 PHAS-I/eIF-4E association was measured as the amount of PHAS-I recovered after purification of translation factor eIF-4E by 7-methyl guanosine triphosphate-Sepharose. Sepharose 156-165 eukaryotic translation initiation factor 4E Rattus norvegicus 7-13 9721171-8 1998 PHAS-I/eIF-4E association was measured as the amount of PHAS-I recovered after purification of translation factor eIF-4E by 7-methyl guanosine triphosphate-Sepharose. Sepharose 156-165 eukaryotic translation initiation factor 4E binding protein 1 Rattus norvegicus 56-62 9721171-8 1998 PHAS-I/eIF-4E association was measured as the amount of PHAS-I recovered after purification of translation factor eIF-4E by 7-methyl guanosine triphosphate-Sepharose. Sepharose 156-165 eukaryotic translation initiation factor 4E Rattus norvegicus 114-120 9773404-6 1998 The expression of HOXD10 relative to beta-tubulin (internal control) was assessed by densitometric comparison of co-amplified Phosphorus-32 (32P) labeled gene products separated by agarose gel electrophoresis. Sepharose 181-188 homeobox D10 Homo sapiens 18-24 9712834-6 1998 By using heparin-Sepharose affinity to discriminate between the monomer and homodimer forms of FGF-1 and resolution by conventional and limited denaturant gel shift immunoblot analysis, it was possible to identify FGF-1 and Syn-1 as potential components of a denaturant- and reducing agent-sensitive extracellular complex. Sepharose 17-26 fibroblast growth factor 1 Bos taurus 95-100 9712834-6 1998 By using heparin-Sepharose affinity to discriminate between the monomer and homodimer forms of FGF-1 and resolution by conventional and limited denaturant gel shift immunoblot analysis, it was possible to identify FGF-1 and Syn-1 as potential components of a denaturant- and reducing agent-sensitive extracellular complex. Sepharose 17-26 fibroblast growth factor 1 Bos taurus 214-219 9698365-4 1998 Heparin-derived disaccharides that could disrupt the IL-8-heparin Sepharose interaction were identified by a competitive binding assay. Sepharose 66-75 C-X-C motif chemokine ligand 8 Homo sapiens 53-57 9714750-7 1998 Our most recent data shows that a fusion protein of CyP-D and glutathione-S-transferase immobilised to Sepharose specifically binds the ANT from Triton-solubilised inner mitochondrial membranes in a cyclosporin A (CsA) sensitive manner. Sepharose 103-112 peptidylprolyl isomerase F Homo sapiens 52-57 9714750-7 1998 Our most recent data shows that a fusion protein of CyP-D and glutathione-S-transferase immobilised to Sepharose specifically binds the ANT from Triton-solubilised inner mitochondrial membranes in a cyclosporin A (CsA) sensitive manner. Sepharose 103-112 solute carrier family 25 member 6 Homo sapiens 136-139 9692396-3 1998 FVIII binding to vWF was measured by capture of patient vWF by polyclonal antibodies on cyanogen bromide-activated Sepharose beads, reaction with recombinant FVIII, and assay of unbound FVIII by clotting methods. Sepharose 115-124 coagulation factor VIII Homo sapiens 0-5 9692396-3 1998 FVIII binding to vWF was measured by capture of patient vWF by polyclonal antibodies on cyanogen bromide-activated Sepharose beads, reaction with recombinant FVIII, and assay of unbound FVIII by clotting methods. Sepharose 115-124 von Willebrand factor Homo sapiens 17-20 9792455-1 1998 In order to study functions of selenoprotein P in human plasma, its level was lowered via two techniques, chromatography on Sepharose-bound heparin, or immunoprecipitation; Western blot analysis showed that both techniques were effective at substantially lowering selenoprotein P levels in plasma. Sepharose 124-133 selenoprotein P Homo sapiens 31-46 9792455-4 1998 Conversely, in a selenoprotein P-enriched plasma preparation obtained via heparin-Sepharose chromatography, protection against benzoate hydroxylation was above controls. Sepharose 82-91 selenoprotein P Homo sapiens 17-32 9693068-7 1998 Detergent-solubilized GST-Sss1p was isolated by adsorption on glutathione-agarose beads. Sepharose 74-81 translocon subunit SSS1 Saccharomyces cerevisiae S288C 26-31 9733580-3 1998 The inhibition of the neoforming capillary network in the chorioallantoic membrane of chick embryo (CAM) was evaluated by agarose disks containing suleparoide and applied on the CAM surface. Sepharose 122-129 calmodulin 2 Gallus gallus 100-103 9733580-7 1998 Likewise, suleparoide 150 microg in agarose disks produced an avascular area of 19.7+/-2.7% of the total area of the CAM (P<0.05 as compared to controls). Sepharose 36-43 calmodulin 3 Homo sapiens 117-120 9668062-16 1998 Reduction of the mucins followed by purification by isopycnic density gradient ultracentrifugation and analysis by agarose gel electrophoresis revealed two bands reacting with an anti-MUC2 tandem repeat antibody after deglycosylation. Sepharose 115-122 mucin 2, oligomeric mucus/gel-forming Homo sapiens 184-188 9675234-1 1998 Ecto-5"-nucleotidase (eNT) from mouse muscle has been purified after extraction with detergent followed by chromatography on concanavalin A- and AMP-Sepharose. Sepharose 149-158 5' nucleotidase, ecto Mus musculus 0-20 9675234-1 1998 Ecto-5"-nucleotidase (eNT) from mouse muscle has been purified after extraction with detergent followed by chromatography on concanavalin A- and AMP-Sepharose. Sepharose 149-158 5' nucleotidase, ecto Mus musculus 22-25 9704765-7 1998 The HLA-DR locus was screened for the presence of the high-risk DRB1*1501 allele by means of selective polymerase chain reaction amplification followed by agarose gel electrophoresis of HLA-DR2 types. Sepharose 155-162 major histocompatibility complex, class II, DR beta 1 Homo sapiens 64-68 9716392-5 1998 UCaM Syn-F2, which binds to calmodulin-Sepharose in a Ca2+-dependent manner, has been purified over 3500-fold in seven steps from rabbit reticulocytes and has a native molecular mass of approximately 620 kDa. Sepharose 39-48 calmodulin Oryctolagus cuniculus 28-38 9692925-1 1998 We have isolated the collagenase/gelatinase activity of fibronectin from a bovine lens capsule hydrolysate, using heparin-agarose, gelatin-agarose, immunopurification with polyclonal antibodies directed against bovine plasma fibronectin, and immunopurification with a monoclonal antibody directed against the extra-domain A of cellular fibronectin. Sepharose 122-129 fibronectin 1 Bos taurus 56-67 9713704-1 1998 Cathepsin D, a lysosomal aspartic protease, has been purified from porcine liver using a combination of pepstatin-A agarose and Affi-Gel Blue affinity chromatography, followed by size-exclusion chromatography. Sepharose 116-123 cathepsin D Sus scrofa 0-11 9692925-1 1998 We have isolated the collagenase/gelatinase activity of fibronectin from a bovine lens capsule hydrolysate, using heparin-agarose, gelatin-agarose, immunopurification with polyclonal antibodies directed against bovine plasma fibronectin, and immunopurification with a monoclonal antibody directed against the extra-domain A of cellular fibronectin. Sepharose 139-146 fibronectin 1 Bos taurus 56-67 9712409-4 1998 Competitive PCR for hAR was then immediately performed on normalized cDNA with a competitor DNA that exhibited a 13 bp deletion as compared to the 163 bp for the target fragment, and the PCR products were easily separated by 3.5% agarose gel electrophoresis. Sepharose 230-237 lymphatic vessel endothelial hyaluronan receptor 1 Homo sapiens 20-23 9614116-3 1998 Both native individual alpha1(V) chains and HepV are eluted at identical NaCl concentrations (0.35 M) from a heparin-Sepharose column, and this binding can be inhibited specifically by the addition of free heparin or heparan sulfate. Sepharose 117-126 collagen type V alpha 1 chain Homo sapiens 23-32 9632802-3 1998 To gain an understanding of the precise mechanism by which p21 affects PCNA function, we have designed a new assay for replication factor C (RFC)-catalyzed loading of PCNA onto DNA, a method that utilizes a primer-template DNA attached to agarose beads via biotin-streptavidin. Sepharose 239-246 cyclin dependent kinase inhibitor 1A Homo sapiens 59-62 9632802-3 1998 To gain an understanding of the precise mechanism by which p21 affects PCNA function, we have designed a new assay for replication factor C (RFC)-catalyzed loading of PCNA onto DNA, a method that utilizes a primer-template DNA attached to agarose beads via biotin-streptavidin. Sepharose 239-246 proliferating cell nuclear antigen Homo sapiens 167-171 9675072-6 1998 Recombinant human gamma-GCS was purified to homogeneity in an overall yield of 45% by ammonium sulfate fractionation followed by sequential chromatography on Q-Sepharose ion-exchange, Superdex 200 gel filtration and ATP-affinity resins. Sepharose 160-169 glutamate-cysteine ligase catalytic subunit Homo sapiens 18-27 9784061-5 1998 The PRV DBP binds cooperatively and preferentially to single-stranded DNA with no significant base preference, judged by agarose gel electrophoresis and competitive nitrocellulose filter binding assays. Sepharose 121-128 D-box binding PAR bZIP transcription factor Homo sapiens 8-11 9632662-0 1998 TIMP-2 promotes activation of progelatinase A by membrane-type 1 matrix metalloproteinase immobilized on agarose beads. Sepharose 105-112 TIMP metallopeptidase inhibitor 2 Homo sapiens 0-6 9632662-0 1998 TIMP-2 promotes activation of progelatinase A by membrane-type 1 matrix metalloproteinase immobilized on agarose beads. Sepharose 105-112 matrix metallopeptidase 2 Homo sapiens 30-45 9632662-0 1998 TIMP-2 promotes activation of progelatinase A by membrane-type 1 matrix metalloproteinase immobilized on agarose beads. Sepharose 105-112 matrix metallopeptidase 14 Homo sapiens 49-89 9632662-3 1998 In this study, a truncated MT1-MMP having a FLAG-tag sequence at the C terminus (MT1-F) was immobilized onto agarose beads (MT1-F/B) and used to analyze the role of TIMP-2. Sepharose 109-116 metallothionein 1F Homo sapiens 81-86 9636226-4 1998 Insulin also increased by severalfold the 32P content of mTOR that was determined after purifying the protein from 32P-labeled adipocytes with rapamycin.FKBP12 agarose beads. Sepharose 160-167 insulin Homo sapiens 0-7 9636226-4 1998 Insulin also increased by severalfold the 32P content of mTOR that was determined after purifying the protein from 32P-labeled adipocytes with rapamycin.FKBP12 agarose beads. Sepharose 160-167 mechanistic target of rapamycin kinase Homo sapiens 57-61 9636226-4 1998 Insulin also increased by severalfold the 32P content of mTOR that was determined after purifying the protein from 32P-labeled adipocytes with rapamycin.FKBP12 agarose beads. Sepharose 160-167 FKBP prolyl isomerase 1A pseudogene 1 Homo sapiens 153-159 9693941-7 1998 ACE genotypes were determined by agarose gel sizing after polymerase chain reaction (PCR) amplification. Sepharose 33-40 angiotensin I converting enzyme Homo sapiens 0-3 9645366-5 1998 Sepharose 4B gel chromatography fractionation followed by density gradient ultracentrifugation revealed both size and density heterogeneity of Thy-1 and Lyn PTK complexes. Sepharose 0-9 Thy-1 cell surface antigen Rattus norvegicus 143-148 9645366-5 1998 Sepharose 4B gel chromatography fractionation followed by density gradient ultracentrifugation revealed both size and density heterogeneity of Thy-1 and Lyn PTK complexes. Sepharose 0-9 LYN proto-oncogene, Src family tyrosine kinase Rattus norvegicus 153-156 9599940-10 1998 Using SDS-agarose gel electrophoresis, we detected 38 APOA isoforms, the highest number recorded to data. Sepharose 10-17 lipoprotein(a) Homo sapiens 54-58 10683997-3 1998 The present study demonstrated that HLP-1, HLP-2 and HLP-3 separated by preparative acid-urea polyacrylamide gel electrophoresis were potently bactericidal against E. coli MI-35P, S. aureus ATCC 25923, P. aeruginosa ATCC 27853 when tested for their antibacteiral activities by using both agarose radial diffusion assay and gel overlay technique. Sepharose 288-295 huntingtin associated protein 1 Homo sapiens 36-41 10683997-3 1998 The present study demonstrated that HLP-1, HLP-2 and HLP-3 separated by preparative acid-urea polyacrylamide gel electrophoresis were potently bactericidal against E. coli MI-35P, S. aureus ATCC 25923, P. aeruginosa ATCC 27853 when tested for their antibacteiral activities by using both agarose radial diffusion assay and gel overlay technique. Sepharose 288-295 hippocalcin like 1 Homo sapiens 43-48 10683997-3 1998 The present study demonstrated that HLP-1, HLP-2 and HLP-3 separated by preparative acid-urea polyacrylamide gel electrophoresis were potently bactericidal against E. coli MI-35P, S. aureus ATCC 25923, P. aeruginosa ATCC 27853 when tested for their antibacteiral activities by using both agarose radial diffusion assay and gel overlay technique. Sepharose 288-295 visinin like 1 Homo sapiens 53-58 9803663-5 1998 Studies on 65Zn2+ binding and zinc chelate Sepharose chromatography showed that monkey serum BChE and human serum BChE have similar characteristics. Sepharose 43-52 butyrylcholinesterase Homo sapiens 93-97 9634083-2 1998 Analysis of binding activity to poly(A)-Sepharose indicated that infected cells contained significant amounts of a protein that co-migrated with ARV protein sigmaNS present in total virus-infected cell extracts. Sepharose 40-49 sigma-NS protein Avian orthoreovirus 157-164 9634083-3 1998 Determination of the N-terminal amino acid sequence of several peptide fragments generated by V8 protease digestion of the poly(A)-Sepharose-purified protein confirmed that this viral protein was sigmaNS. Sepharose 131-140 sigma-NS protein Avian orthoreovirus 196-203 9634083-4 1998 Competition assays showed that single-stranded RNA from the unrelated avian pathogen infectious bursal disease virus was able to compete for binding of sigmaNS to poly(A)-Sepharose. Sepharose 171-180 sigma-NS protein Avian orthoreovirus 152-159 9703362-10 1998 The dominant pi class form in adenomas could also be identified as p-1 by its binding to S-hexylglutathione-Sepharose. Sepharose 108-117 zinc finger protein 185 Mus musculus 67-70 9655178-1 1998 Chromatography of human myeloperoxidase (MPO) on a heparin-agarose column demonstrated a tight association of the protein with the resin. Sepharose 59-66 myeloperoxidase Homo sapiens 24-39 9655178-1 1998 Chromatography of human myeloperoxidase (MPO) on a heparin-agarose column demonstrated a tight association of the protein with the resin. Sepharose 59-66 myeloperoxidase Homo sapiens 41-44 9631524-5 1998 The GST-Bax fusion protein was bound to glutathione-Sepharose, and Bax was released by thrombin cleavage and further purified by sequential chromatography on heparin-Sepharose and DEAE-Sepharose. Sepharose 52-61 BCL2 associated X, apoptosis regulator Homo sapiens 8-11 9631524-5 1998 The GST-Bax fusion protein was bound to glutathione-Sepharose, and Bax was released by thrombin cleavage and further purified by sequential chromatography on heparin-Sepharose and DEAE-Sepharose. Sepharose 166-175 BCL2 associated X, apoptosis regulator Homo sapiens 8-11 9631524-5 1998 The GST-Bax fusion protein was bound to glutathione-Sepharose, and Bax was released by thrombin cleavage and further purified by sequential chromatography on heparin-Sepharose and DEAE-Sepharose. Sepharose 166-175 BCL2 associated X, apoptosis regulator Homo sapiens 67-70 9593830-3 1998 In the LBS-Lp(a) immunoassay, minimal changes in the LBS activity of Lp(a) were observed after modification with lipoprotein lipase, sphingomyelinase, or phospholipase C. In contrast, a significant (p<0.003) increase in the LBS activity of Lp(a) occurred after phospholipase A2 (PLA2) treatment, and this increase was confirmed using the lysine-Sepharose bead assay. Sepharose 348-357 lipoprotein(a) Homo sapiens 69-74 9593830-3 1998 In the LBS-Lp(a) immunoassay, minimal changes in the LBS activity of Lp(a) were observed after modification with lipoprotein lipase, sphingomyelinase, or phospholipase C. In contrast, a significant (p<0.003) increase in the LBS activity of Lp(a) occurred after phospholipase A2 (PLA2) treatment, and this increase was confirmed using the lysine-Sepharose bead assay. Sepharose 348-357 lipoprotein(a) Homo sapiens 69-74 9659399-3 1998 Recombinant mGBP3 protein, expressed using a baculovirus expression system, binds to agarose-immobilized guanine nucleotides (GTP, GDP and GMP). Sepharose 85-92 guanylate binding protein 3 Mus musculus 12-17 9572990-5 1998 An association between hSIE and Stat-3 after MHC-I ligation was directly demonstrated by precipitating Stat-3 from nuclear extracts with biotinylated hSIE probe and avidin-coupled agarose. Sepharose 180-187 signal transducer and activator of transcription 3 Homo sapiens 32-38 9560321-1 1998 Macrophage-migration-inhibitory factor (MIF) is retained by S-hexylglutathione-agarose but is not specifically eluted in high yield. Sepharose 79-86 macrophage migration inhibitory factor Homo sapiens 0-38 9560321-1 1998 Macrophage-migration-inhibitory factor (MIF) is retained by S-hexylglutathione-agarose but is not specifically eluted in high yield. Sepharose 79-86 macrophage migration inhibitory factor Homo sapiens 40-43 9632894-5 1998 The cytochrome P-450scc form whose amino groups are not accessible to the modifier is retained on the affinity matrix, and after elution from adrenodoxin-Sepharose has the absorption spectrum typical of the high-spin protein with a spectral homogeneity index A392/A278 = 1.0. Sepharose 154-163 cytochrome P450 family 11 subfamily A member 1 Homo sapiens 15-23 9634038-3 1998 Using reverse transcriptase-polymerase chain reaction, mRNA corresponding to the UPTF1 portion of prothrombin was analysed by agarose-gel electrophoresis and Southern blotting. Sepharose 126-133 coagulation factor II, thrombin Homo sapiens 98-109 9581823-2 1998 We now report that pro-uPA-Sepharose-purified uPAR from ascites of patients with ovarian carcinoma is the full-length molecule missing the glycosyl-phosphatidylinositol anchor, as determined by its amino acid composition. Sepharose 27-36 plasminogen activator, urokinase receptor Homo sapiens 46-50 9629899-1 1998 In an attempt to subfractionate apolipoprotein B-containing lipoproteins (apoB-Lp), agarose isoelectric focusing (IEF) of apoB-Lp in human sera was carried out in the presence of two kinds of cyclodextrins (CDs), which have different affinities for cholesterol. Sepharose 84-91 apolipoprotein B Homo sapiens 122-126 9629899-2 1998 ApoB-Lp in the agarose gels was detected by immunofixation, followed by protein staining. Sepharose 15-22 apolipoprotein B Homo sapiens 0-4 9653632-5 1998 In this study, fractionated acid-washed gelatin was cleaved with trypsin and resultant peptides fractionated by fibronectin-Sepharose affinity chromatography. Sepharose 124-133 fibronectin 1 Homo sapiens 112-123 9610467-8 1998 RESULTS: PCR products, resolved by electrophoresis on agarose gels, showed four bands corresponding to four discrete, alternatively spliced forms of the FSH receptor. Sepharose 54-61 follicle stimulating hormone receptor Mus musculus 153-165 9599809-6 1998 Immunoaffinity chromatography using anti-mzFKBP66 demonstrated an association of the protein with an unknown 36-kDa polypeptide, and affinity chromatography of mzFKBP-66 on calmodulin-agarose beads indicated the presence of a calmodulin-binding site. Sepharose 184-191 calmodulin1 Zea mays 173-183 9848182-2 1998 Biospecific adsorbents for thrombin and antithrombin III (AT III) isolation were synthesized with heparin, phyllophoran and furcellaran as ligands on aminopropylsilochrome and this proteins sorption characteristics on this sorbents in comparisons with heparin-cellulose and heparin-sepharose were investigated. Sepharose 282-291 coagulation factor II, thrombin Homo sapiens 27-35 9848182-2 1998 Biospecific adsorbents for thrombin and antithrombin III (AT III) isolation were synthesized with heparin, phyllophoran and furcellaran as ligands on aminopropylsilochrome and this proteins sorption characteristics on this sorbents in comparisons with heparin-cellulose and heparin-sepharose were investigated. Sepharose 282-291 serpin family C member 1 Homo sapiens 40-56 9848182-2 1998 Biospecific adsorbents for thrombin and antithrombin III (AT III) isolation were synthesized with heparin, phyllophoran and furcellaran as ligands on aminopropylsilochrome and this proteins sorption characteristics on this sorbents in comparisons with heparin-cellulose and heparin-sepharose were investigated. Sepharose 282-291 serpin family C member 1 Homo sapiens 58-64 9679332-1 1998 The glyceraldehyde-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) was purified to homogeneity from electric organ of Electrophorus electricus (L.) by a hydrophobic chromatography method on deacetylcolchicine-Sepharose. Sepharose 206-215 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 44-49 9553113-9 1998 One eIF4E isoform, IFE-1, was also retained on m32,2,7GTP-Sepharose. Sepharose 58-67 Eukaryotic translation initiation factor 4E-1;eIF-4F 25 kDa subunit Caenorhabditis elegans 19-24 9553113-10 1998 Furthermore, binding of IFE-1 and IFE-2 to m7GTP-Sepharose was inhibited by m32,2,7GTP. Sepharose 49-58 Eukaryotic translation initiation factor 4E-1;eIF-4F 25 kDa subunit Caenorhabditis elegans 24-29 9553113-10 1998 Furthermore, binding of IFE-1 and IFE-2 to m7GTP-Sepharose was inhibited by m32,2,7GTP. Sepharose 49-58 Eukaryotic translation initiation factor 4E-2 Caenorhabditis elegans 34-39 9553114-7 1998 Agarose gel electrophoresis revealed that unlike human apoAIV, which migrates to a pre-alpha-position, chicken apoAIV shows fast alpha migration. Sepharose 0-7 apolipoprotein A4 Gallus gallus 111-117 9545355-3 1998 The presence of an approximately 120-kDa soluble leptin receptor in mouse plasma was confirmed by precipitation with leptin-Sepharose beads followed by immunobloting with anti-leptin receptor antibodies. Sepharose 124-133 leptin receptor Mus musculus 49-64 9648916-8 1998 PAI-1 mRNA levels in NRK cells maintained in serum- and CD-free agarose suspension culture were low or undetectable. Sepharose 64-71 serpin family E member 2 Rattus norvegicus 0-5 9604329-6 1998 GFP-C/EBP also binds to DNA-Sepharose which contains the CAAT element and is eluted by a salt gradient. Sepharose 28-37 CCAAT/enhancer binding protein gamma Rattus norvegicus 4-9 9558116-4 1998 Affinity purification using factor H Sepharose followed by immunoprecipitation using mAbs to various integrin chains identified Mac-1 (CD11b/CD18) as a factor H binding receptor. Sepharose 37-46 integrin subunit alpha M Homo sapiens 128-133 9558116-4 1998 Affinity purification using factor H Sepharose followed by immunoprecipitation using mAbs to various integrin chains identified Mac-1 (CD11b/CD18) as a factor H binding receptor. Sepharose 37-46 complement factor H Homo sapiens 152-160 9598988-2 1998 PDI dimers bound to CNBr-activated Sepharose were shown to possess high TPOR activity as well as the ability to reactivate lysozyme. Sepharose 35-44 prolyl 4-hydroxylase subunit beta Homo sapiens 0-3 9598988-2 1998 PDI dimers bound to CNBr-activated Sepharose were shown to possess high TPOR activity as well as the ability to reactivate lysozyme. Sepharose 35-44 MPL proto-oncogene, thrombopoietin receptor Homo sapiens 72-76 9535885-2 1998 The reconstructions of an intermediate form of human alpha2-macroglobulin (half-transformed alpha2M) in which two of its four bait regions and thiol ester sites were cleaved by chymotrypsin bound to Sepharose were obtained by three-dimensional electron microscopy from stain and frozen-hydrated specimens. Sepharose 199-208 alpha-2-macroglobulin Homo sapiens 53-73 9535885-2 1998 The reconstructions of an intermediate form of human alpha2-macroglobulin (half-transformed alpha2M) in which two of its four bait regions and thiol ester sites were cleaved by chymotrypsin bound to Sepharose were obtained by three-dimensional electron microscopy from stain and frozen-hydrated specimens. Sepharose 199-208 alpha-2-macroglobulin Homo sapiens 92-99 9537999-10 1998 Clusterin in human serum bound to heparin-Sepharose at pH 6.0 but not at pH 7.4. Sepharose 42-51 clusterin Homo sapiens 0-9 9537999-11 1998 Dot-blot experiments showed that one of the polypeptide chains of clusterin which had been reduced and alkylated under denaturing conditions bound to heparin-Sepharose. Sepharose 158-167 clusterin Homo sapiens 66-75 9525888-6 1998 In vitro synthesized FLI bound to actin-Sepharose and binding was reduced by competition with excess soluble actin. Sepharose 40-49 FLII actin remodeling protein Homo sapiens 21-24 9525888-6 1998 In vitro synthesized FLI bound to actin-Sepharose and binding was reduced by competition with excess soluble actin. Sepharose 40-49 Actin 79B Drosophila melanogaster 34-39 9525946-2 1998 We reported that MCM protein complexes consisting of MCM2, -4, -6, and -7 bind strongly to a histone-Sepharose column (Ishimi, Y., Ichinose, S., Omori, A., Sato, K., and Kimura, H. (1996) J. Biol. Sepharose 101-110 minichromosome maintenance complex component 7 Homo sapiens 17-20 9525946-2 1998 We reported that MCM protein complexes consisting of MCM2, -4, -6, and -7 bind strongly to a histone-Sepharose column (Ishimi, Y., Ichinose, S., Omori, A., Sato, K., and Kimura, H. (1996) J. Biol. Sepharose 101-110 minichromosome maintenance complex component 2 Mus musculus 53-57 9525959-3 1998 The membrane-associated platelet CKII kinase was partially purified using heparin-agarose, phosphocellulose, and ion exchange chromatography. Sepharose 82-89 casein kinase 2 alpha 1 Homo sapiens 33-37 9579441-2 1998 STUDY DESIGN: The level of urea, creatinine, electrolytes, enzymes, total protein, and alpha-fetoprotein and the affinity of alpha-fetoprotein for concanavalin A Sepharose was measured in samples of vesicular fluid from complete (n=2) and partial (n=1) mole, nuchal fluid (n=4), and cystic hygroma fluid (n=4). Sepharose 162-171 alpha fetoprotein Homo sapiens 125-142 9635924-3 1998 However, combined treatment with cisplatin and IFN-gamma induced apoptosis in macrophages as studied by percent DNA fragmentation assay, qualitative analysis of DNA on agarose gel electrophoresis, and morphological and nuclear alterations studied by phase contrast and fluorescence microscopy. Sepharose 168-175 interferon gamma Mus musculus 47-56 9576010-9 1998 The presence of soluble B7-1 was confirmed by immunoprecipitation using PSRM-3-coupled Sepharose beads. Sepharose 87-96 CD80 molecule Homo sapiens 24-28 9578489-3 1998 The gene for human chymase was cloned in a baculovirus vector and expressed in High Five insect cells, and the recombinant protein purified by heparin-agarose and gel-filtration chromatography. Sepharose 151-158 chymase 1 Homo sapiens 19-26 9762585-3 1998 Our results indicate that G4 AChE expressed at embryonic day (ED) 9 and ED15 could be purified by acridinium-Sepharose chromatography and shared similar biochemical and kinetic properties with the adult form. Sepharose 109-118 acetylcholinesterase Mus musculus 29-33 9538257-1 1998 A chymotrypsin-like proteinase, designated myonase, was successfully purified to homogeneity from X-chromosome linked muscular dystrophic mouse skeletal muscle by affinity chromatography on agarose conjugated with lima bean trypsin inhibitor as ligand. Sepharose 190-197 mast cell protease 4 Mus musculus 43-50 9538264-1 1998 Cathepsin A was purified approximately 550-fold with an overall yield of 4% from bovine spleen crude extracts by successive chromatographies on DEAE-Sephadex A-50, phenyl-Toyopearl 650C, and Con A-agarose. Sepharose 197-204 cathepsin A Bos taurus 0-11 9523576-3 1998 The expressed protein was localized to inclusion bodies, and varying the growth parameters resulted in the solubilization of small amounts of GST-MOG that could be affinity purified on glutathione agarose columns. Sepharose 197-204 myelin oligodendrocyte glycoprotein Homo sapiens 146-149 9516457-4 1998 Binding studies were performed using bFGF immobilized on Sepharose beads and soluble 125I-labeled fibrinogen and also using Sepharose-immobilized fibrinogen and soluble 125I-bFGF. Sepharose 124-133 fibrinogen beta chain Homo sapiens 146-156 9521730-5 1998 Specifically, recombinant human galectin-3 was found to bind to galectin-3C (the carboxyl-terminal domain fragment) conjugated to Sepharose 4B and the binding was inhibitable by lactose. Sepharose 130-139 galectin 3 Homo sapiens 32-42 9507013-12 1998 rPLD1 bound to concanavalin A-Sepharose beads, and its electrophoretic mobility was altered by treatment with endoglycosidase F. The amount of PLD bound to the beads was decreased in a concentration-dependent manner when tunicamycin was added to the Sf9 expression system. Sepharose 30-39 phospholipase D1 Rattus norvegicus 0-5 9507013-12 1998 rPLD1 bound to concanavalin A-Sepharose beads, and its electrophoretic mobility was altered by treatment with endoglycosidase F. The amount of PLD bound to the beads was decreased in a concentration-dependent manner when tunicamycin was added to the Sf9 expression system. Sepharose 30-39 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 1-4 9494087-7 1998 Alternatively, surface-biotinylated GLUT4 could be easily, but less sensitively, detected in streptavidin agarose precipitates which were analysed by conventional GLUT4 Western blotting. Sepharose 106-113 solute carrier family 2 member 4 Rattus norvegicus 36-41 9521842-1 1998 The novel microtubule-interacting protein Mip-90 was originally isolated from HeLa cells by using affinity columns of agarose derivatized with peptides from the C-terminal regulatory domain on beta-tubulin. Sepharose 118-125 major intrinsic protein of lens fiber Homo sapiens 42-45 9532348-4 1998 Genotype of the 12-bp repeat polymorphism of DRD4 was determined with polymerase chain reaction and agarose gel electrophoresis. Sepharose 100-107 dopamine receptor D4 Homo sapiens 45-49 9539166-1 1998 Apo-myoglobin covalently linked on CNBr-activated Sepharose 4B is proposed as a new heme acceptor for investigating the heme transfer reaction from hemoproteins. Sepharose 50-62 myoglobin Homo sapiens 4-13 9522942-6 1998 A segment of the myophosphorylase gene containing the mutation site was amplified by polymerase chain reaction assays, and the genotype (normal vs affected allele) was determined by using restriction enzyme and agarose gel electrophoretic analysis. Sepharose 211-218 glycogen phosphorylase, muscle associated Bos taurus 17-33 9480888-3 1998 In order to investigate this, we isolated LPL from bovine milk via heparin Sepharose chromatography using a continuous salt gradient. Sepharose 75-84 lipoprotein lipase Bos taurus 42-45 9563831-6 1998 cTnI is extracted from small bovine, rabbit and human heart tissue samples (30-100 mg) under special conditions avoiding dephosphorylation and is isolated by affinity chromatography on cTnC Sepharose. Sepharose 190-199 troponin I3, cardiac type Homo sapiens 0-4 9500454-5 1998 For 0.5 microM Pc 4 and either 2.1 or 3 kJ/m2, which kill 90 or 99.9% of the cells, oligonucleosomal fragmentation was visible on agarose gels as early as 60 or 30 min after PDT, respectively. Sepharose 130-137 proprotein convertase subtilisin/kexin type 4 Mus musculus 15-19 9523852-4 1998 We developed a new cell haptotactic/attachment assay by using Thr and Fib covalently bound to Sepharose beads (SBs). Sepharose 94-103 fibrinogen beta chain Homo sapiens 70-73 10920955-2 1998 METHODS: The growth and gene expression of HCE16/3 cells treated with IGF-I and epidermal growth factor (EGF) were examined by flow cytometry, soft-agarose assay and RT-PCR analysis. Sepharose 148-155 insulin like growth factor 1 Homo sapiens 70-75 10920955-2 1998 METHODS: The growth and gene expression of HCE16/3 cells treated with IGF-I and epidermal growth factor (EGF) were examined by flow cytometry, soft-agarose assay and RT-PCR analysis. Sepharose 148-155 epidermal growth factor Homo sapiens 80-103 10920955-2 1998 METHODS: The growth and gene expression of HCE16/3 cells treated with IGF-I and epidermal growth factor (EGF) were examined by flow cytometry, soft-agarose assay and RT-PCR analysis. Sepharose 148-155 epidermal growth factor Homo sapiens 105-108 10920955-6 1998 Furthermore, the combined use of EGF and IGF-I could induce colony formation of HCE16/3 cells in soft agarose. Sepharose 102-109 epidermal growth factor Homo sapiens 33-36 10920955-6 1998 Furthermore, the combined use of EGF and IGF-I could induce colony formation of HCE16/3 cells in soft agarose. Sepharose 102-109 insulin like growth factor 1 Homo sapiens 41-46 9494024-5 1998 Using thrombin-agarose affinity chromatography, (DD)E and fragment E but not D-dimer were identified as the thrombin-binding fibrin fragments, indicating that the thrombin-binding site is located within the E domain. Sepharose 15-22 coagulation factor II, thrombin Homo sapiens 6-14 9461514-5 1998 PP1c and associated proteins were isolated by microcystin-Sepharose. Sepharose 58-67 protein phosphatase 1 catalytic subunit gamma Homo sapiens 0-4 9540793-5 1998 Consistent with studies on lipoprotein lipase, the denatured rHL eluted from heparin-Sepharose at a lower salt concentration of 0.42 M NaCl than the native rHL which eluted at 0.72 M NaCl. Sepharose 85-94 hes family bHLH transcription factor 1 Rattus norvegicus 61-64 9538935-10 1998 FGF-2 administered with heparin was the most effective method of enhancing angiogenesis as compared to FGF-2 alone, FGF-2 plus heparan sulfate, or FGF-2 coated heparin agarose beads. Sepharose 168-175 fibroblast growth factor 2 Sus scrofa 0-5 9491903-7 1998 Lp(a) was removed from plasma by sepharose coupled anti-Lp(a) columns. Sepharose 33-42 lipoprotein(a) Homo sapiens 0-5 9516927-3 1998 Pgp function was determined using PSC833 in a drug resistance-reversal assay and with a three-dimensional agarose-based extreme drug resistance assay. Sepharose 106-113 ATP binding cassette subfamily B member 1 Homo sapiens 0-3 9445371-6 1998 The study with a heparin-Sepharose column showed that 3T3-L1 adipocytes contained three forms of LPL. Sepharose 25-34 lipoprotein lipase Homo sapiens 97-100 9495520-11 1998 This value approximated that of phosphatidic acid liposomes and of phosvitin cross-linked to agarose beads, and it might reflect the energetics of heterogeneous nucleation on a highly phosphorylated surface. Sepharose 93-100 casein kinase 2 beta Bos taurus 67-76 9396813-1 1997 The identity of DNA replication proteins and cell cycle regulatory proteins which can be found in complexes involving PCNA were investigated by the use of PCNA immobilized on Sepharose 4B. Sepharose 175-187 proliferating cell nuclear antigen Bos taurus 118-122 9487529-5 1998 One of the ligands binds IgG competitively with SpA in solution and when immobilized on agarose beads, with an affinity constant of 10(5)-10(6) M-1. Sepharose 88-95 surfactant protein A2 Homo sapiens 48-51 9490776-0 1998 Purification of the Plasma Membrane Ca2+-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography The Ca2+-ATPase of the plasma membrane (PM) of germinating radish (Raphanus sativus L.) seeds was purified by calmodulin (CaM)-affinity chromatography using a batch procedure. Sepharose 84-91 autoinhibited Ca2+-ATPase 1 Arabidopsis thaliana 36-47 9490776-0 1998 Purification of the Plasma Membrane Ca2+-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography The Ca2+-ATPase of the plasma membrane (PM) of germinating radish (Raphanus sativus L.) seeds was purified by calmodulin (CaM)-affinity chromatography using a batch procedure. Sepharose 84-91 calmodulin Raphanus sativus 73-83 9490776-0 1998 Purification of the Plasma Membrane Ca2+-ATPase from Radish Seedlings by Calmodulin-Agarose Affinity Chromatography The Ca2+-ATPase of the plasma membrane (PM) of germinating radish (Raphanus sativus L.) seeds was purified by calmodulin (CaM)-affinity chromatography using a batch procedure. Sepharose 84-91 autoinhibited Ca2+-ATPase 1 Arabidopsis thaliana 120-131 9490776-1 1998 PM purified by aqueous two-phase partitioning was solubilized with n-dodecyl beta-d-maltoside and applied to a CaM-agarose matrix. Sepharose 115-122 calmodulin Raphanus sativus 111-114 9454608-8 1998 Use of Adx--Sepharose affinity matrix yielded < 90% pure P450MT2 (specific activity: 13.5 nmol/mg of protein) starting from a partially purified fraction of 10-15% purity, further demonstrating the specificity of P450MT2 and Adx interaction. Sepharose 12-21 ferredoxin 1 Homo sapiens 7-10 9422715-4 1998 xSmad2, generated either by in vitro translation or by overexpression in COS cells, specifically bound to calmodulin-agarose; the association was calcium-dependent and required xSmad2 N-terminal residues. Sepharose 117-124 calmodulin 1 Homo sapiens 106-116 9422715-5 1998 In the same assay, xSmad1 and hSmads 2, 3, and 4 also bound to calmodulin-agarose. Sepharose 74-81 calmodulin 1 Homo sapiens 63-73 9613705-12 1998 Myoglobin was isolated from human heart extract by immunoaffinity chromatography on Sepharose-antibody no. Sepharose 84-93 myoglobin Homo sapiens 0-9 10319528-6 1998 DNA topoisomerase I relaxing activity was determined on supercoiled plasmid DNA, and topoisomers separated by agarose gel electrophoresis. Sepharose 110-117 DNA topoisomerase I Rattus norvegicus 0-19 10806763-1 1998 Phenotyping of erythrocyte EsD and PGM1 enzymes was performed by a mixed agarose-starch gel electrophoresis in 165 healthy blood donors of Changsha District. Sepharose 73-80 phosphoglucomutase 1 Homo sapiens 35-39 10076829-8 1998 The characteristics of electrophoretic binding of HSA on Blue Sepharose Fast Flow are examined. Sepharose 62-71 albumin Homo sapiens 50-53 9595551-3 1998 Cell surface localization of the Tg-induced GRP 78 was confirmed by biotinylation of membrane-exposed proteins and subsequent isolation of the biotin-labelled proteins by streptavidin/agarose affinity chromatography. Sepharose 184-191 heat shock protein family A (Hsp70) member 5 Homo sapiens 44-50 9769931-0 1998 [Determination of alpha 1 antitrypsin phenotypes in plasma using isoelectric focusing on this agarose gel]. Sepharose 94-101 serpin family A member 1 Homo sapiens 18-37 9405391-1 1997 The human alpha1,3-fucosyltransferase, Fuc-TVII, a key enzyme in the biosynthesis of selectin ligands, was expressed as a soluble protein-A chimeric form in a human B cell lymphoma cell line, Namalwa KJM-1, and purified using IgG-Sepharose. Sepharose 230-239 fucosyltransferase 7 Homo sapiens 10-37 9405391-1 1997 The human alpha1,3-fucosyltransferase, Fuc-TVII, a key enzyme in the biosynthesis of selectin ligands, was expressed as a soluble protein-A chimeric form in a human B cell lymphoma cell line, Namalwa KJM-1, and purified using IgG-Sepharose. Sepharose 230-239 fucosyltransferase 7 Homo sapiens 39-47 9405398-2 1997 p37 was expressed as a glutathione S-transferase fusion protein and was purified to homogeneity by silver staining using glutathione-agarose, Sephacryl S-200, and DEAE-cellulose chromatography. Sepharose 133-140 nucleoporin 37 Homo sapiens 0-3 18642336-2 1997 PDI and PPI were successfully immobilized on cross-linked agarose beads. Sepharose 58-65 protein disulfide isomerase family A member 2 Homo sapiens 0-3 9548595-11 1998 Heparin-Sepharose chromatography of wild-type LPL and a mutant LPL in which the well-characterized heparin-binding sequence (Arg 281-Lys 282-Arg 284) has been mutated was carried out in the presence and absence of xCAL 3-6a. Sepharose 8-17 lipoprotein lipase Bos taurus 46-49 9548595-11 1998 Heparin-Sepharose chromatography of wild-type LPL and a mutant LPL in which the well-characterized heparin-binding sequence (Arg 281-Lys 282-Arg 284) has been mutated was carried out in the presence and absence of xCAL 3-6a. Sepharose 8-17 lipoprotein lipase Bos taurus 63-66 9531454-4 1998 Polymerase chain reaction using "hot start" and restriction enzyme digestion by HhaI was used for genotyping of apoE alleles, which were detected by polyacrylamide or MetaPhor agarose gel. Sepharose 176-183 apolipoprotein E Homo sapiens 112-116 9446560-10 1998 (ii) Galactomannan proteins can be precipitated with agarose-immobilized alpha-galactose-specific lectin from a whole cell lysate prepared from transformed mnn1 cells grown in a galactose medium. Sepharose 53-60 alpha-1,3-mannosyltransferase MNN1 Saccharomyces cerevisiae S288C 156-160 9592052-4 1998 ApoJ mRNA levels were increased in both cortex (77%, p < 0.01) and hippocampus (64%, p < 0.02), whereas, in contrast to previous findings 3 days after KA injection, DNA fragmentation was not detected on agarose gel electrophoresis. Sepharose 209-216 clusterin Rattus norvegicus 0-4 9544731-5 1998 NO donor, S-Nitroso-n-acetylpenicillamine (SNAP) or 3-morpholinosydnonimine (SIN-1) (0.1-1.0 mmol/l) dose-dependently reduced copper-induced oxidation of LDL as demonstrated by the inhibition of electrophoretic mobilities on agarose gels and the formation of thiobarbituric acid-reactive substances (TBARS) and conjugated dienes. Sepharose 225-232 MAPK associated protein 1 Homo sapiens 77-82 9544731-6 1998 Moreover, treatment with IL-1beta (5-50 ng/ml) reduced the increases in electrophoretic mobilities on agarose gels and TBARS formation in association with increases in NO production. Sepharose 102-109 interleukin 1 beta Homo sapiens 25-33 9699011-5 1998 Agarose gel electrophoresis of TGF-beta 1-treated hepatocytes shows a typical ladder-like pattern of DNA fragments and PMA, a selective stimulator of protein kinase C, diminishes the DNA fragmentation and cell death. Sepharose 0-7 transforming growth factor beta 1 Homo sapiens 31-41 9484958-3 1998 We report a procedure designed to purify actin from nonmuscle tissues employing extraction of monomeric actin from tissues with high concentrations of Tris, chromatography on DE-53 cellulose, and affinity chromatography of DNase I-agarose. Sepharose 231-238 actin epsilon 1 Bos taurus 41-46 9484958-3 1998 We report a procedure designed to purify actin from nonmuscle tissues employing extraction of monomeric actin from tissues with high concentrations of Tris, chromatography on DE-53 cellulose, and affinity chromatography of DNase I-agarose. Sepharose 231-238 deoxyribonuclease 1 Bos taurus 223-230 10452831-5 1998 We found that 1-10 ng/ml of hrIL-6 inhibited proliferation to approximately 65% of control levels and suppressed colony formation induced by bFGF in soft agarose. Sepharose 154-161 fibroblast growth factor 2 Homo sapiens 141-145 10322660-3 1998 CK-MM isoforms were separated within 30 minutes by electrophoresis on agarose gel with a discontinuous buffer system at constant current of 30 mA and low voltage of 200-300 V, then measured by fluorescence scanning. Sepharose 70-77 creatine kinase, M-type Homo sapiens 0-5 9541209-4 1998 Agarose beads soaked in growth factors or pieces of mouse mandibular epithelium (E11) were placed in contact with E11 molar-forming mesenchyme and cultured for 24 h. Whole-mount in situ hybridization analysis revealed that, in contrast to mouse mandibular epithelium and BMP-4-releasing beads, EGF-releasing beads did not induce the expression of Msx-1 and Msx-2 in E11 molar-forming mesenchyme. Sepharose 0-7 bone morphogenetic protein 4 Mus musculus 271-276 9541209-4 1998 Agarose beads soaked in growth factors or pieces of mouse mandibular epithelium (E11) were placed in contact with E11 molar-forming mesenchyme and cultured for 24 h. Whole-mount in situ hybridization analysis revealed that, in contrast to mouse mandibular epithelium and BMP-4-releasing beads, EGF-releasing beads did not induce the expression of Msx-1 and Msx-2 in E11 molar-forming mesenchyme. Sepharose 0-7 epidermal growth factor Mus musculus 294-297 9541209-4 1998 Agarose beads soaked in growth factors or pieces of mouse mandibular epithelium (E11) were placed in contact with E11 molar-forming mesenchyme and cultured for 24 h. Whole-mount in situ hybridization analysis revealed that, in contrast to mouse mandibular epithelium and BMP-4-releasing beads, EGF-releasing beads did not induce the expression of Msx-1 and Msx-2 in E11 molar-forming mesenchyme. Sepharose 0-7 msh homeobox 1 Mus musculus 347-352 9541209-4 1998 Agarose beads soaked in growth factors or pieces of mouse mandibular epithelium (E11) were placed in contact with E11 molar-forming mesenchyme and cultured for 24 h. Whole-mount in situ hybridization analysis revealed that, in contrast to mouse mandibular epithelium and BMP-4-releasing beads, EGF-releasing beads did not induce the expression of Msx-1 and Msx-2 in E11 molar-forming mesenchyme. Sepharose 0-7 msh homeobox 2 Mus musculus 357-362 9813410-4 1998 MBL variants were detected by subsequent restriction enzyme digestion and agarose gel electrophoresis. Sepharose 74-81 mannose binding lectin 2 Homo sapiens 0-3 10072635-7 1998 Semiquantitative C4-alpha-chain estimates of relative isotype levels correlated well with the number of alleles seen at each locus by agarose gel electrophoresis, and were superior to other isotype quantitation methods. Sepharose 134-141 complement C4A (Rodgers blood group) Homo sapiens 17-25 10028315-1 1998 In order to provide patients with von Willebrand disease a factor VIII (FVIII)/von Willebrand factor (vWF) concentrate of reproducible quality, an SDS-agarose gel electrophoresis method has been established to determine the content of the high molecular weight multimers (band 11 and higher) of vWF. Sepharose 151-158 von Willebrand factor Homo sapiens 102-105 9452308-4 1998 The carbohydrate recognition domain of microglia-derived galectin-3 was functional as the molecule could be affinity purified on lactose-agarose. Sepharose 137-144 lectin, galactose binding, soluble 3 Mus musculus 57-67 9484897-7 1998 LcrG could bind directly to heparin-agarose beads and, in agreement with these results, analysis of the protein sequence of Yersinia enterocolitica LcrG revealed heparin-binding motifs. Sepharose 36-43 control of Yop release Yersinia enterocolitica 0-4 9663461-2 1998 Here, we report that RT-PCR amplification of human caudal medulla RNA generated a distinct band on agarose gels corresponding in size and sequence to the predicted 742-bp POMC PCR product. Sepharose 99-106 proopiomelanocortin Homo sapiens 171-175 9523381-9 1998 PCR amplification showed that CENP-B adsorbed on a Sepharose-4B/anti-CENP-B antibody column retained alphaDNA satellites. Sepharose 51-60 centromere protein B Homo sapiens 30-36 9492146-1 1998 We have prepared a biotinylated thyrotropin receptor (TSHR-BIO), and characterized its activity in cells and when bound to solid phase (streptavidin agarose). Sepharose 149-156 thyroid stimulating hormone receptor Homo sapiens 32-52 9492146-1 1998 We have prepared a biotinylated thyrotropin receptor (TSHR-BIO), and characterized its activity in cells and when bound to solid phase (streptavidin agarose). Sepharose 149-156 thyroid stimulating hormone receptor Homo sapiens 54-58 9492146-8 1998 We have shown the application of streptavidin agarose immobilized TSHR-BIO for the detection of thyroid-binding inhibiting immunoglobulines in unfractionated sera. Sepharose 46-53 thyroid stimulating hormone receptor Homo sapiens 66-70 9396813-6 1997 The DNA replication proteins which bound to PCNA-Sepharose included DNA polymerase delta and straightepsilon, PCNA, the 37 and 40 kDa subunits of RFC, the 70 kDa subunit of RPA, NDH II and topoisomerase I. Sepharose 49-58 proliferating cell nuclear antigen Bos taurus 44-48 9396813-6 1997 The DNA replication proteins which bound to PCNA-Sepharose included DNA polymerase delta and straightepsilon, PCNA, the 37 and 40 kDa subunits of RFC, the 70 kDa subunit of RPA, NDH II and topoisomerase I. Sepharose 49-58 proliferating cell nuclear antigen Bos taurus 110-114 9396813-6 1997 The DNA replication proteins which bound to PCNA-Sepharose included DNA polymerase delta and straightepsilon, PCNA, the 37 and 40 kDa subunits of RFC, the 70 kDa subunit of RPA, NDH II and topoisomerase I. Sepharose 49-58 DExH-box helicase 9 Bos taurus 155-184 9416791-8 1997 Furthermore, this PARP inhibitor was shown to significantly increase the amount of DNA fragmentation as revealed by the DNA migration pattern in agarose gel electrophoresis. Sepharose 145-152 poly (ADP-ribose) polymerase family, member 1 Mus musculus 18-22 9425282-0 1997 Identification of an autocrine chondrocyte colony-stimulating factor: chondromodulin-I stimulates the colony formation of growth plate chondrocytes in agarose culture. Sepharose 151-158 leukocyte cell-derived chemotaxin 1 Oryctolagus cuniculus 70-86 9425282-5 1997 In the present study, we observed that ChM-I stimulates the colony formation of rabbit growth plate chondrocytes in agarose culture. Sepharose 116-123 leukocyte cell-derived chemotaxin 1 Oryctolagus cuniculus 39-44 9395481-1 1997 Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) was identified as a low abundance protein in bovine uterus that co-purified with estrogen receptor (ER) in a ligand-independent manner and was separated from the ER by its lower retention on estrogen response element (ERE)-Sepharose. Sepharose 290-299 nuclear receptor subfamily 2 group F member 1 Bos taurus 0-56 9395481-1 1997 Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) was identified as a low abundance protein in bovine uterus that co-purified with estrogen receptor (ER) in a ligand-independent manner and was separated from the ER by its lower retention on estrogen response element (ERE)-Sepharose. Sepharose 290-299 nuclear receptor subfamily 2 group F member 1 Bos taurus 58-65 9395522-7 1997 Deglycosylation experiments, reactivity with lectins, and chromatography on concanavalin A-Sepharose indicated that corneodesmosin is N-glycosylated. Sepharose 91-100 corneodesmosin Homo sapiens 116-130 9398266-9 1997 Recombinant 43 kDa 5-phosphatase bound to recombinant glutathione S-transferase (GST)/14-3-3zeta fusion protein, but not GST alone, immobilized on glutathione-Sepharose. Sepharose 159-168 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta Homo sapiens 86-96 9398266-13 1997 In addition, platelet cytosolic 5-phosphatase bound to recombinant 14-3-3zeta immobilized on glutathione-Sepharose. Sepharose 105-114 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta Homo sapiens 67-77 9398268-6 1997 Furthermore, it was shown that agarose-coupled NCAM-antibodies retained 85% of the ATPase activity released from synaptosomes after treatment with phosphatidylinositol-specific phospholipase C. These findings restricted the association or expression of the enzymatic activity to the extracellular part of NCAM. Sepharose 31-38 neural cell adhesion molecule 1 Rattus norvegicus 47-51 9398268-6 1997 Furthermore, it was shown that agarose-coupled NCAM-antibodies retained 85% of the ATPase activity released from synaptosomes after treatment with phosphatidylinositol-specific phospholipase C. These findings restricted the association or expression of the enzymatic activity to the extracellular part of NCAM. Sepharose 31-38 neural cell adhesion molecule 1 Rattus norvegicus 305-309 9435686-10 1997 To demonstrate that OP-1 can exert both stimulatory and inhibitory effects within a developing kidney, embryonic explants were treated with agarose beads saturated with 2 microM OP-1. Sepharose 140-147 bone morphogenetic protein 7 Mus musculus 20-24 9390170-1 1997 In the present study, using the C24 bile acid chenodeoxycholic acid as substrate, rat liver bile acid CoA ligase activity (rBAL) was purified 200-fold from detergent-solubilized microsomes using a combination of Q-Sepharose anion exchange, hydroxyapatite, and CM-Sepharose chromatography. Sepharose 214-223 solute carrier family 27 member 5 Rattus norvegicus 123-127 9390170-1 1997 In the present study, using the C24 bile acid chenodeoxycholic acid as substrate, rat liver bile acid CoA ligase activity (rBAL) was purified 200-fold from detergent-solubilized microsomes using a combination of Q-Sepharose anion exchange, hydroxyapatite, and CM-Sepharose chromatography. Sepharose 263-272 solute carrier family 27 member 5 Rattus norvegicus 123-127 9390170-4 1997 A monoclonal antibody raised against the 65-kDa protein and covalently coupled to 6B-Sepharose completely absorbed rBAL activity from a semipurified preparation of rat liver microsomes. Sepharose 85-94 solute carrier family 27 member 5 Rattus norvegicus 115-119 9390173-3 1997 The binding of most cartilage lysozyme isoforms and hen egg-white lysozyme (control) to chondroitin sulfate and hyaluronan linked to agarose supports displayed optimal levels at approximately 20 and 5-10 mM salt, respectively, but decreased at both lower and higher salt concentrations indicating the electrostatic nature of the interactions. Sepharose 133-140 lysozyme C, tracheal isozyme Bos taurus 30-38 9390173-3 1997 The binding of most cartilage lysozyme isoforms and hen egg-white lysozyme (control) to chondroitin sulfate and hyaluronan linked to agarose supports displayed optimal levels at approximately 20 and 5-10 mM salt, respectively, but decreased at both lower and higher salt concentrations indicating the electrostatic nature of the interactions. Sepharose 133-140 lysozyme C, tracheal isozyme Bos taurus 66-74 9440622-6 1997 When the cells were incubated with apoptosis-inducing anti-Fas-Ag monoclonal antibody, agarose gel electrophoresis of the control cells yielded a typical pattern of DNA fragmentations. Sepharose 87-94 Fas (TNF receptor superfamily member 6) Mus musculus 59-65 9428743-1 1997 Cytosolic glyoxalase II from Arabidopsis thaliana, GLX2-2, was overexpressed and purified to homogeneity using Q-sepharose chromatography. Sepharose 113-122 Metallo-hydrolase/oxidoreductase superfamily protein Arabidopsis thaliana 10-23 9428743-1 1997 Cytosolic glyoxalase II from Arabidopsis thaliana, GLX2-2, was overexpressed and purified to homogeneity using Q-sepharose chromatography. Sepharose 113-122 Metallo-hydrolase/oxidoreductase superfamily protein Arabidopsis thaliana 51-57 9498561-1 1997 Endo-beta-N-acetylglucosaminidase from hen oviduct (Endo-HO) was purified to homogeneity by ammonium sulfate fractionation and then by column chromatographies on DEAE-Sephacel, hydroxyapatite, Octyl-Sepharose CL-4B, Co2+-chelating Sepharose FF, and YMC-Pack Diol-200G. Sepharose 199-208 endo-beta-N-acetylglucosaminidase Homo sapiens 0-33 9389772-5 1997 The antibody was further affinity-purified on MP78/70 bound to Sepharose and used to localize the protein in a range of bovine tissues. Sepharose 63-72 transforming growth factor beta induced Bos taurus 46-53 9433481-3 1997 In the study reported here, exposure of primary human skin fibroblasts to PAF for 1 h in serum-free medium was shown to cause sustained proliferation over 50 d in medium containing low serum and anchorage-independent growth in soft agarose. Sepharose 232-239 PCNA clamp associated factor Homo sapiens 74-77 9400613-10 1997 Intraspecies PKR interactions were more efficient than interspecies PKR interactions, and interactions between RNA-binding-sufficient PKR proteins were more efficient than those involving an RNA-binding mutant as measured by binding to GST-PKR protein Sepharose beads. Sepharose 252-261 glutathione S-transferase kappa 1 Homo sapiens 236-239 9400613-12 1997 Purified mouse full-length PKR(1-515)WT GST fusion protein retained kinase activity on Sepharose beads, but the activity was not impaired by association with either the full-length or the N-terminal region of human PKR. Sepharose 87-96 eukaryotic translation initiation factor 2-alpha kinase 2 Mus musculus 27-30 9400613-12 1997 Purified mouse full-length PKR(1-515)WT GST fusion protein retained kinase activity on Sepharose beads, but the activity was not impaired by association with either the full-length or the N-terminal region of human PKR. Sepharose 87-96 glutathione S-transferase kappa 1 Homo sapiens 40-43 9453215-4 1997 The Drosophila nAChR, isolated with three putative subunits (69, 66 and 61 kDa) using a nicotinoid-agarose affinity column, is labeled by [125I]AN primarily at the 66 kDa subunit and secondarily at the 61 kDa subunit. Sepharose 99-106 nicotinic Acetylcholine Receptor beta1 Drosophila melanogaster 15-20 9358157-3 1997 The resulting recombinant protein (Pus4) was expressed at high level and purified to homogeneity by metal affinity chromatography on Ni2+-NTA-agarose, followed by ion-exchange chromatography on MonoQ. Sepharose 142-149 pseudouridine synthase PUS4 Saccharomyces cerevisiae S288C 35-39 9395244-10 1997 DNA polymerase alpha specifically bound to a purified Rb protein-immobilized Sepharose column. Sepharose 77-86 DNA polymerase alpha 1, catalytic subunit Homo sapiens 0-20 9395244-11 1997 Rb protein also bound to DNA polymerase alpha trapped to anti-DNA polymerase alpha antibody-Sepharose column, suggesting the direct association of these two proteins. Sepharose 92-101 DNA polymerase alpha 1, catalytic subunit Homo sapiens 25-45 9395244-11 1997 Rb protein also bound to DNA polymerase alpha trapped to anti-DNA polymerase alpha antibody-Sepharose column, suggesting the direct association of these two proteins. Sepharose 92-101 DNA polymerase alpha 1, catalytic subunit Homo sapiens 62-82 9367892-6 1997 In accordance with this result YY1 was specifically precipitated from nuclear extracts by ADPRT immobilized on sepharose. Sepharose 111-120 YY1 transcription factor Homo sapiens 31-34 9367892-6 1997 In accordance with this result YY1 was specifically precipitated from nuclear extracts by ADPRT immobilized on sepharose. Sepharose 111-120 poly(ADP-ribose) polymerase 1 Homo sapiens 90-95 9367896-2 1997 We have revealed that several proteins, including alpha adaptins which are specific subunits of clathrin assembly protein, AP2, were eluted from mouse brain by affinity elution chromatography from the C2 domains of Syt II-immobilized Sepharose using 50 microM of InsP6. Sepharose 234-243 transcription factor AP-2, alpha Mus musculus 123-126 9367896-2 1997 We have revealed that several proteins, including alpha adaptins which are specific subunits of clathrin assembly protein, AP2, were eluted from mouse brain by affinity elution chromatography from the C2 domains of Syt II-immobilized Sepharose using 50 microM of InsP6. Sepharose 234-243 SS18, nBAF chromatin remodeling complex subunit Mus musculus 215-218 9305914-2 1997 Our group purified MCM protein complexes consisting of MCM2, -4 (Cdc21), -6 (Mis5), and -7 (CDC47) proteins from HeLa cells by using histone-Sepharose column chromatography (Ishimi, Y., Ichinose, S., Omori, A., Sato K., and Kimura, H. (1996) J. Biol. Sepharose 141-150 minichromosome maintenance complex component 7 Homo sapiens 19-22 9361024-7 1997 Binding of HAP1 to p150 Glued (amino acids 879-1150) was confirmed in vitro by binding of p150 Glued to a HAP1-GST fusion protein immobilized on glutathione-Sepharose beads. Sepharose 157-166 huntingtin-associated protein 1 Rattus norvegicus 11-15 9361024-7 1997 Binding of HAP1 to p150 Glued (amino acids 879-1150) was confirmed in vitro by binding of p150 Glued to a HAP1-GST fusion protein immobilized on glutathione-Sepharose beads. Sepharose 157-166 dynactin subunit 1 Rattus norvegicus 19-29 9361024-7 1997 Binding of HAP1 to p150 Glued (amino acids 879-1150) was confirmed in vitro by binding of p150 Glued to a HAP1-GST fusion protein immobilized on glutathione-Sepharose beads. Sepharose 157-166 dynactin subunit 1 Rattus norvegicus 90-100 9344469-0 1997 Escherichia coli flavodoxin sepharose as an affinity resin for cytochromes P450 and use to identify a putative cytochrome P450c17/3beta-hydroxysteroid dehydrogenase interaction. Sepharose 28-37 steroid 17-alpha-hydroxylase/17,20 lyase Bos taurus 122-129 9344469-1 1997 Flavodoxin Sepharose (Fld Sepharose), a reagent originally developed to demonstrate an interaction between native Escherichia coli Fld and cytochrome P450c17, has been synthesized, using highly expressed (7 micromol Fld/liter E. coli culture) recombinant E. coli Fld, for use as an affinity resin for microsomal cytochromes P450. Sepharose 11-20 steroid 17-alpha-hydroxylase/17,20 lyase Bos taurus 150-157 9344469-1 1997 Flavodoxin Sepharose (Fld Sepharose), a reagent originally developed to demonstrate an interaction between native Escherichia coli Fld and cytochrome P450c17, has been synthesized, using highly expressed (7 micromol Fld/liter E. coli culture) recombinant E. coli Fld, for use as an affinity resin for microsomal cytochromes P450. Sepharose 26-35 steroid 17-alpha-hydroxylase/17,20 lyase Bos taurus 150-157 9344469-4 1997 Electrophoretic and immunoblot analyses of fractions eluted from the Fld Sepharose column revealed the presence of P450c17 and P450c21, both of which were sufficiently pure, after SDS-PAGE, for identification by N-terminal sequence analysis. Sepharose 73-82 steroid 17-alpha-hydroxylase/17,20 lyase Bos taurus 115-122 9344469-5 1997 Intriguingly, a major protein copurifying with P450c17 and P450c21 was identified as 3beta-hydroxysteroid dehydrogenase (3beta-HSD) which was subsequently found not to directly bind Fld Sepharose. Sepharose 186-195 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Bos taurus 121-130 9344469-6 1997 Purified bovine 3beta-HSD covalently linked to Sepharose can bind recombinant bovine P450c17, an interaction which is partially disrupted upon mild heat denaturation of P450c17 or by the nonionic detergent Emulgen. Sepharose 47-56 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Bos taurus 16-25 9344469-6 1997 Purified bovine 3beta-HSD covalently linked to Sepharose can bind recombinant bovine P450c17, an interaction which is partially disrupted upon mild heat denaturation of P450c17 or by the nonionic detergent Emulgen. Sepharose 47-56 steroid 17-alpha-hydroxylase/17,20 lyase Bos taurus 85-92 9344469-6 1997 Purified bovine 3beta-HSD covalently linked to Sepharose can bind recombinant bovine P450c17, an interaction which is partially disrupted upon mild heat denaturation of P450c17 or by the nonionic detergent Emulgen. Sepharose 47-56 steroid 17-alpha-hydroxylase/17,20 lyase Bos taurus 169-176 9345039-4 1997 Both rvWF and pdvWF caused increases in factor VIII, which were sustained even when vWF:Ag had decreased to nearly undetectable levels and only monomeric or dimeric species were detectable on agarose gels. Sepharose 192-199 von Willebrand factor Canis lupus familiaris 6-9 22358898-3 1997 The (His)(6)-LECT2 was purified from the soluble fraction in E. coli as a single band in sodium dodesyl sulfate/polyacrylamide gel electrophoresis using three steps of column chromatography with Ni(2+)-charged nitrilo-triacetic acid (Ni-NTA) agarose, DEAE-Sepharose, and CM-Sepharose. Sepharose 242-249 leukocyte cell derived chemotaxin 2 Homo sapiens 13-18 9362347-3 1997 By immunoblotting, using a monoclonal antibody raised against the HGF alpha-subunit, the bile HGF, which was purified on a Heparin-Sepharose column, showed a band of the same size as the recombinant HGF alpha-subunit (69 kd). Sepharose 131-140 hepatocyte growth factor Homo sapiens 66-69 9362347-3 1997 By immunoblotting, using a monoclonal antibody raised against the HGF alpha-subunit, the bile HGF, which was purified on a Heparin-Sepharose column, showed a band of the same size as the recombinant HGF alpha-subunit (69 kd). Sepharose 131-140 hepatocyte growth factor Homo sapiens 94-97 9362347-3 1997 By immunoblotting, using a monoclonal antibody raised against the HGF alpha-subunit, the bile HGF, which was purified on a Heparin-Sepharose column, showed a band of the same size as the recombinant HGF alpha-subunit (69 kd). Sepharose 131-140 hepatocyte growth factor Homo sapiens 94-97 9413555-1 1997 The milk protein alpha-lactalbumin was isolated from bovine whey protein concentrate solution by immobilized metal ion affinity chromatography (IMAC) using Cu(II)-Chelating Sepharose Fast Flow. Sepharose 173-182 Weaning weight-maternal milk Bos taurus 4-8 9413555-1 1997 The milk protein alpha-lactalbumin was isolated from bovine whey protein concentrate solution by immobilized metal ion affinity chromatography (IMAC) using Cu(II)-Chelating Sepharose Fast Flow. Sepharose 173-182 lactalbumin alpha Bos taurus 17-34 9341124-7 1997 Both tenascin-C and TNfn4,5 were retained on Sepharose 4B-linked carboxyl-terminal neurocan domains, which in BIAcore binding studies yielded a KD value of 17 nM for purified tenascin-C. Sepharose 45-54 tenascin C Homo sapiens 5-15 9341124-7 1997 Both tenascin-C and TNfn4,5 were retained on Sepharose 4B-linked carboxyl-terminal neurocan domains, which in BIAcore binding studies yielded a KD value of 17 nM for purified tenascin-C. Sepharose 45-54 tenascin C Homo sapiens 175-185 9325262-10 1997 Additional support for reciprocal binding was obtained in fluorescence experiments where both probes were labeled and in experiments monitoring ligand binding to agarose-immobilized thrombin. Sepharose 162-169 coagulation factor II, thrombin Homo sapiens 182-190 9324935-1 1997 In this paper, we report a fast, simple, and reproducible staining protocol for nucleic acids in agarose gels with a sensitivity in the order of 10 pg/mm2. Sepharose 97-104 PNMA family member 2 Homo sapiens 151-154 9324946-7 1997 Enzymatically active complexes of HA-CaM and MLCK could be immunoprecipitated from actomyosin preparations using the same monoclonal antibody and protein G-Sepharose beads. Sepharose 156-165 calmodulin 2 Gallus gallus 37-40 9324946-7 1997 Enzymatically active complexes of HA-CaM and MLCK could be immunoprecipitated from actomyosin preparations using the same monoclonal antibody and protein G-Sepharose beads. Sepharose 156-165 myosin light chain kinase Gallus gallus 45-49 16130275-1 1997 Naturally occurring forms of hepatocyte growth factor/scatter factor (HGF/SF) have been purified by heparin-Sepharose chromatography, followed by cation exchange chromatography from a pool of human seminal plasma. Sepharose 108-117 hepatocyte growth factor Homo sapiens 29-53 16130275-2 1997 Using an enzyme-linked immunosorbant assay, MDCK scatter assay, and Western blot analysis, it was found that, after heparin-Sepharose chromatography, human HGF/SF present in seminal plasma eluted in two different fractions, between 0.72 and 0.85 M NaCl (fraction I) and between 0.95 and 1.10 M NaCl (fraction II). Sepharose 124-133 hepatocyte growth factor Homo sapiens 156-162 9441485-3 1997 We characterized a gel-electrophoretic system based on MS-4 agarose (and its correspondence to its passport) and tested its fitness for standard molecular genetic expert investigations. Sepharose 60-67 MS4 Homo sapiens 55-59 9441485-0 1997 [The electrophoretic properties of gels of high-resolution MS-4 agarose for separating DNA fragments in molecular genetic analysis]. Sepharose 64-71 MS4 Homo sapiens 59-63 9441485-5 1997 Important features of MS-4 agarose gels were detected, not typical of other agarose systems, which should be borne in mind during investigations with this agarose. Sepharose 27-34 MS4 Homo sapiens 22-26 9441485-5 1997 Important features of MS-4 agarose gels were detected, not typical of other agarose systems, which should be borne in mind during investigations with this agarose. Sepharose 76-83 MS4 Homo sapiens 22-26 9441485-5 1997 Important features of MS-4 agarose gels were detected, not typical of other agarose systems, which should be borne in mind during investigations with this agarose. Sepharose 76-83 MS4 Homo sapiens 22-26 10923398-2 1998 METHODS: Immunoadsorbent against TNF-alpha was produced by the attachment of anti-TNF-alpha monoclonal antibody (McAb) to agarose beads. Sepharose 122-129 tumor necrosis factor Oryctolagus cuniculus 33-42 10923398-2 1998 METHODS: Immunoadsorbent against TNF-alpha was produced by the attachment of anti-TNF-alpha monoclonal antibody (McAb) to agarose beads. Sepharose 122-129 tumor necrosis factor Oryctolagus cuniculus 82-91 9385634-4 1997 AAPH treatment caused a time-dependent decrease in the number of functional Lp(a) or r-apo(a) molecules capable of binding to fibrin or lysine-Sepharose and in the intrinsic protein fluorescence of both Lp(a) and r-apo(a). Sepharose 143-152 lipoprotein(a) Homo sapiens 76-81 9367170-1 1997 Prostate specific antigen (uPSA) was purified to homogeneity from human urine using SuperQ-Toyopearl, Sulfate-Cellulofine, Phenyl-Toyopearl, CM-Sepharose, anti-urokinase IgG Sepharose and Sephadex G-100. Sepharose 144-153 kallikrein related peptidase 3 Homo sapiens 0-32 9367170-1 1997 Prostate specific antigen (uPSA) was purified to homogeneity from human urine using SuperQ-Toyopearl, Sulfate-Cellulofine, Phenyl-Toyopearl, CM-Sepharose, anti-urokinase IgG Sepharose and Sephadex G-100. Sepharose 174-183 kallikrein related peptidase 3 Homo sapiens 0-32 9391721-2 1997 Binding competition assays of plasminogen and uPA to heparin-sepharose demonstrated that heparin bound to both enzymes. Sepharose 61-70 plasminogen activator, urokinase Mus musculus 46-49 9420169-0 1997 Detection and semiquantitation of albumin forms in fresh human plasma separated on gradient polyacrylamide gel by means of electroblotting on agarose gel matrix. Sepharose 142-149 albumin Homo sapiens 34-41 9420169-6 1997 After staining of the agarose gel, it revealed the presence of seven stained bands of albumin in addition to the monomer. Sepharose 22-29 albumin Homo sapiens 86-93 9393978-1 1997 Wild type human tumor suppressor protein p53 (expressed in insect cells) binds strongly to negatively supercoiled (sc) plasmid DNA at a native superhelix density, as evidenced by electrophoretic retardation of scDNA in agarose gels and imaging by scanning force microscopy (SFM). Sepharose 219-226 TSC complex subunit 1 Homo sapiens 16-32 9393978-1 1997 Wild type human tumor suppressor protein p53 (expressed in insect cells) binds strongly to negatively supercoiled (sc) plasmid DNA at a native superhelix density, as evidenced by electrophoretic retardation of scDNA in agarose gels and imaging by scanning force microscopy (SFM). Sepharose 219-226 tumor protein p53 Homo sapiens 41-44 9393978-12 1997 The above results are not restricted to human p53; the wild type rat p53 protein also results in the retardation of scDNA on agarose gels. Sepharose 125-132 Wistar clone pR53P1 p53 pseudogene Rattus norvegicus 69-72 9299527-5 1997 Because the enzyme kinetics indicated a PLA2-melittin interaction, a melittin-sepharose affinity column was used to purify a PLA2 from human serum. Sepharose 78-87 phospholipase A2 group IIA Homo sapiens 125-129 9299551-6 1997 Furthermore, UTI forms multi-polymers in the presence of Ca2+ as demonstrated by gel filtration and agarose gel electrophoresis. Sepharose 100-107 alpha-1-microglobulin/bikunin precursor Homo sapiens 13-16 9299551-6 1997 Furthermore, UTI forms multi-polymers in the presence of Ca2+ as demonstrated by gel filtration and agarose gel electrophoresis. Sepharose 100-107 carbonic anhydrase 2 Homo sapiens 57-60 9236937-2 1997 A 52 kDa maltose binding-AgB fusion protein (rAgB.MBP) was produced and inclusion bodies containing the fusion protein were solubilised in urea and affinity purified on an amylose-Sepharose 6B column. Sepharose 180-189 Ras-related GTP binding B Rattus norvegicus 45-49 9307037-1 1997 A protein that neutralizes the biological activities of basic phospholipase A2 (PLA2) myotoxin isoforms from the venom of the snake Bothrops asper was isolated from its blood by affinity chromatography with Sepharose-immobilized myotoxins. Sepharose 207-216 phospholipase A2 group IB Homo sapiens 80-84 9308888-2 1997 It was found that highly active 2,3-DPGM can be extracted from human erythrocyte hemolysates in a complex with GAPDH adsorbed on Sepharose-bound anti-GAPDH antibodies at pH 6.5, the molar ratio being one 2,3-GPGM subunit per subunit of GAPDH. Sepharose 129-138 bisphosphoglycerate mutase Homo sapiens 36-40 9308888-2 1997 It was found that highly active 2,3-DPGM can be extracted from human erythrocyte hemolysates in a complex with GAPDH adsorbed on Sepharose-bound anti-GAPDH antibodies at pH 6.5, the molar ratio being one 2,3-GPGM subunit per subunit of GAPDH. Sepharose 129-138 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 111-116 9308888-2 1997 It was found that highly active 2,3-DPGM can be extracted from human erythrocyte hemolysates in a complex with GAPDH adsorbed on Sepharose-bound anti-GAPDH antibodies at pH 6.5, the molar ratio being one 2,3-GPGM subunit per subunit of GAPDH. Sepharose 129-138 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 150-155 9308888-2 1997 It was found that highly active 2,3-DPGM can be extracted from human erythrocyte hemolysates in a complex with GAPDH adsorbed on Sepharose-bound anti-GAPDH antibodies at pH 6.5, the molar ratio being one 2,3-GPGM subunit per subunit of GAPDH. Sepharose 129-138 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 150-155 9308888-6 1997 The Kd values of the complexes determined with purified enzyme preparations were in the range 2.40-2.48 microM for both the GAPDH x 2,3-DPGM and GAPGH x 3-PGK enzyme pairs, when titrations of GAPDH covalently bound to CNBr-activated Sepharose were performed by the soluble 2,3-DPGM or 3-PGK. Sepharose 233-242 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 124-129 9308888-6 1997 The Kd values of the complexes determined with purified enzyme preparations were in the range 2.40-2.48 microM for both the GAPDH x 2,3-DPGM and GAPGH x 3-PGK enzyme pairs, when titrations of GAPDH covalently bound to CNBr-activated Sepharose were performed by the soluble 2,3-DPGM or 3-PGK. Sepharose 233-242 bisphosphoglycerate mutase Homo sapiens 132-140 9308888-6 1997 The Kd values of the complexes determined with purified enzyme preparations were in the range 2.40-2.48 microM for both the GAPDH x 2,3-DPGM and GAPGH x 3-PGK enzyme pairs, when titrations of GAPDH covalently bound to CNBr-activated Sepharose were performed by the soluble 2,3-DPGM or 3-PGK. Sepharose 233-242 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 192-197 9308888-6 1997 The Kd values of the complexes determined with purified enzyme preparations were in the range 2.40-2.48 microM for both the GAPDH x 2,3-DPGM and GAPGH x 3-PGK enzyme pairs, when titrations of GAPDH covalently bound to CNBr-activated Sepharose were performed by the soluble 2,3-DPGM or 3-PGK. Sepharose 233-242 bisphosphoglycerate mutase Homo sapiens 273-281 9281592-5 1997 Chondroadherin-binding proteins from chondrocyte lysates were affinity purified on chondroadherin-Sepharose. Sepharose 98-107 chondroadherin Homo sapiens 0-14 9281592-5 1997 Chondroadherin-binding proteins from chondrocyte lysates were affinity purified on chondroadherin-Sepharose. Sepharose 98-107 chondroadherin Homo sapiens 83-97 9299453-3 1997 The decrease in calnexin gene expression by HT-29 cells growing as colonies in soft agarose was confirmed by reverse transcriptase-PCR (RT-PCR) using calnexin-specific primers. Sepharose 84-91 calnexin Homo sapiens 16-24 9413156-1 1997 Interleukin-18 (IL-18) induces apoptosis in human myelomonocytic KG-1 cells as determined by agarose gel electrophoresis, and flow cytometry after propidium iodide (PI) staining. Sepharose 93-100 interleukin 18 Homo sapiens 0-14 9413156-1 1997 Interleukin-18 (IL-18) induces apoptosis in human myelomonocytic KG-1 cells as determined by agarose gel electrophoresis, and flow cytometry after propidium iodide (PI) staining. Sepharose 93-100 interleukin 18 Homo sapiens 16-21 9327767-3 1997 Type III HTG-VLDL were oxidized as measured by both conjugated-diene formation and increased electrophoretic mobility on agarose gels. Sepharose 121-128 CD320 antigen Mus musculus 13-17 9457766-3 1997 A protein which specifically and reversibly binds to human [125I]PRL is isolated from the pooled fractions of HMW-irPRL by affinity chromatography on prolactin-Sepharose. Sepharose 160-169 cilia and flagella associated protein 97 Homo sapiens 110-113 9313931-5 1997 P-glycoprotein was immunoprecipitated with a monoclonal antibody (MRK-16) and the P-glycoprotein-MRK-16-Protein A-Sepharose complexes obtained were subjected to a coupled enzyme ATPase assay. Sepharose 114-123 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9313931-5 1997 P-glycoprotein was immunoprecipitated with a monoclonal antibody (MRK-16) and the P-glycoprotein-MRK-16-Protein A-Sepharose complexes obtained were subjected to a coupled enzyme ATPase assay. Sepharose 114-123 ATP binding cassette subfamily B member 1 Homo sapiens 82-96 9332326-3 1997 TNF alpha-induced cell death showed an apoptotic pattern, as detected by agarose electrophoresis, flow cytometry and transmission electron microscopy (TEM). Sepharose 73-80 tumor necrosis factor Homo sapiens 0-9 9275069-2 1997 By affinity chromatography on agarose-IGF-I and HPLC, truncated IGFBP-2 of apparent Mr 14,000-16,000 resolved into two peaks. Sepharose 30-37 insulin like growth factor 1 Homo sapiens 38-43 9275069-2 1997 By affinity chromatography on agarose-IGF-I and HPLC, truncated IGFBP-2 of apparent Mr 14,000-16,000 resolved into two peaks. Sepharose 30-37 insulin like growth factor binding protein 2 Homo sapiens 64-71 9306058-6 1997 Equine MMP-2 was purified from synovial fibroblast cell culture supernatant, and equine MMP-9 from polymorph neutrophil cell culture supernatant, by gelatin-sepharose affinity chromatography. Sepharose 157-166 matrix metallopeptidase 2 Equus caballus 7-12 9346305-4 1997 Free PCI and tissue-kallikrein x PCI complexes but not free tissue kallikrein bound to heparin-Sepharose, implying that the inhibitory effect of heparin cannot be caused by a tissue-kallikrein-heparin interaction. Sepharose 95-104 serpin family A member 5 Homo sapiens 5-8 9322737-7 1997 Moreover, the GST-vRaf fusion protein could be readily purified from Xenopus extracts using glutathione Sepharose. Sepharose 104-113 hematopoietic prostaglandin D synthase S homeolog Xenopus laevis 14-17 9363706-7 1997 HSP70 mRNA was detected in HeLa cells by a reverse transcriptase-polymerase chain reaction (RT-PCR) and analysis on agarose gels. Sepharose 116-123 heat shock protein family A (Hsp70) member 4 Homo sapiens 0-5 9378530-6 1997 SDS-PAGE on 12.5 per cent gels of eluates obtained from calmodulin-agarose columns, followed by overlay of corresponding western blots with biotinylated calmodulin revealed the presence of a 68 kDa calmodulin binding protein in samples collected either at 28 wk or at full-term of pregnancy. Sepharose 67-74 calmodulin 2 Mus musculus 56-66 9349753-4 1997 Platelet-activating factor acetylhydrolase was partially purified from bovine seminal plasma using gelatin-agarose and ion-exchange chromatography and nondenaturing polyacrylamide gel electrophoresis (PAGE). Sepharose 107-114 phospholipase A2 group VII Bos taurus 0-42 9378778-4 1997 Ethidium bromide/agarose gel electrophoresis on the cytosolic fraction of BME cells revealed a basal level of fragmented DNA that was increased by anti-bFGF antibodies to an extent not exceeding that observed in parallel cultures incubated with concentrations of transforming growth factor-ss1 that increase VEGF-induced in vitro angiogenesis. Sepharose 17-24 fibroblast growth factor 2 Bos taurus 152-156 9449207-4 1997 The results of both assays indicate that ERE-purified ER, with its four associated proteins (hsp70, PDI, p48, p45), has a greater ability to bind to the vitellogenin A2 ERE than ER purified by estradiol-Sepharose chromatography in the absence (ESeph) or presence (EATP) of ATP, in which p48, p45 (ESeph) and hsp70 (EATP) are removed. Sepharose 203-212 estrogen receptor 1 Homo sapiens 41-43 9307035-2 1997 The cDNA encoding human glutathione S-transferase (GST) T1 has been expressed as two recombinant forms in Escherichia coli that could be purified by affinity chromatography on either IgG-Sepharose or nickel-agarose; one form of the transferase was synthesized from the pALP 1 expression vector as a Staphylococcus aureus protein A fusion, whereas the other form was synthesized from the pET-20b expression vector as a C-terminal polyhistidine-tagged recombinant. Sepharose 187-196 glutathione S-transferase theta 1 Homo sapiens 24-58 9307035-2 1997 The cDNA encoding human glutathione S-transferase (GST) T1 has been expressed as two recombinant forms in Escherichia coli that could be purified by affinity chromatography on either IgG-Sepharose or nickel-agarose; one form of the transferase was synthesized from the pALP 1 expression vector as a Staphylococcus aureus protein A fusion, whereas the other form was synthesized from the pET-20b expression vector as a C-terminal polyhistidine-tagged recombinant. Sepharose 207-214 glutathione S-transferase theta 1 Homo sapiens 24-58 9278336-9 1997 BRN-1 anti-MCR1/MCR2 Ab immunoprecipitated proteins of 125 and 150 kDa from surface-radiolabeled mouse platelets, which specifically bound to C3d-Sepharose. Sepharose 146-155 POU domain, class 3, transcription factor 3 Mus musculus 0-5 9278336-9 1997 BRN-1 anti-MCR1/MCR2 Ab immunoprecipitated proteins of 125 and 150 kDa from surface-radiolabeled mouse platelets, which specifically bound to C3d-Sepharose. Sepharose 146-155 melanocortin 1 receptor Mus musculus 11-15 9298970-15 1997 The interaction between S100A1 and the purified RYR complex was also investigated by affinity chromatography: in the presence of nanomolar Ca2+, we observed binding of native RYR complex to S100A1-conjugated Sepharose. Sepharose 208-217 protein S100-A1 Oryctolagus cuniculus 24-30 9298970-15 1997 The interaction between S100A1 and the purified RYR complex was also investigated by affinity chromatography: in the presence of nanomolar Ca2+, we observed binding of native RYR complex to S100A1-conjugated Sepharose. Sepharose 208-217 protein S100-A1 Oryctolagus cuniculus 190-196 9449207-4 1997 The results of both assays indicate that ERE-purified ER, with its four associated proteins (hsp70, PDI, p48, p45), has a greater ability to bind to the vitellogenin A2 ERE than ER purified by estradiol-Sepharose chromatography in the absence (ESeph) or presence (EATP) of ATP, in which p48, p45 (ESeph) and hsp70 (EATP) are removed. Sepharose 203-212 estrogen receptor 1 Homo sapiens 54-56 9264546-3 1997 Recombinant NACP purified through heat treatment, DEAE-Sephacel anion-exchange, Sephacryl S-200 size-exclusion, and S-Sepharose cation-exchange chromatography steps appeared as a single band on SDS-PAGE with Mr of 19 kDa. Sepharose 116-127 synuclein alpha Homo sapiens 12-16 9295164-1 1997 A phospholipase D1 (PLD1) was purified from rat brain by the use of antibody-coupled protein A Sepharose. Sepharose 95-104 phospholipase D1 Rattus norvegicus 2-18 9295164-1 1997 A phospholipase D1 (PLD1) was purified from rat brain by the use of antibody-coupled protein A Sepharose. Sepharose 95-104 phospholipase D1 Rattus norvegicus 20-24 9268686-4 1997 The recombinant Fuc-TVII could be highly purified in a single-step purification procedure, i.e., IgG-Sepharose column chromatography. Sepharose 101-110 fucosyltransferase 7 Homo sapiens 16-24 9242707-4 1997 Using this recombinant substrate we developed a novel agarose cell culture system containing either rat chondrosarcoma or bovine chondrocytes that could be used in studies of the biochemical characterization of aggrecanase activities. Sepharose 54-61 ADAM metallopeptidase with thrombospondin type 1 motif, 4 Rattus norvegicus 211-222 9244377-1 1997 Chromatography of extracts from rat liver membranes on wheat-germ lectin-Sepharose resulted in a partial resolution of the insulin receptor from other phosphorylatable proteins. Sepharose 73-82 insulin receptor Rattus norvegicus 123-139 9259426-11 1997 Addition of recombinant Gas-6 to serum-free medium resulted in increased survival of primary chondrocytes cultured at low density in agarose. Sepharose 133-140 growth arrest specific 6 Homo sapiens 24-29 9300133-1 1997 Carboxylesterases (EC 3.1.1.1) from human liver were purified using Q-Sepharose, Sephadex G-150, isoelectrofocusing and Con A-Sepharose. Sepharose 70-79 carboxylesterase 2 Homo sapiens 0-17 9301123-1 1997 We have identified 2 DnaK and 3 GroEL homologues from moderately halophilic Acinetobacter, Pseudomonas, and Planococcus species by partial purification using an ATP-agarose column and by the analysis and similarity search of these NH2-terminal amino acid sequences. Sepharose 165-172 heat shock protein family D (Hsp60) member 1 Homo sapiens 32-37 9378707-8 1997 GST-AICAR transformylase can be purified to homogeneity by a single-step affinity procedure with glutathione Sepharose. Sepharose 109-118 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase Homo sapiens 4-9 9242510-9 1997 Heparitinase pretreatment retarded, whereas endo-beta-galactosidase enhanced the mobility of the > or = 180-kDa polydisperse CD44 on agarose gel electrophoresis. Sepharose 136-143 galactosidase beta 1 Homo sapiens 49-67 9242510-9 1997 Heparitinase pretreatment retarded, whereas endo-beta-galactosidase enhanced the mobility of the > or = 180-kDa polydisperse CD44 on agarose gel electrophoresis. Sepharose 136-143 CD44 molecule (Indian blood group) Homo sapiens 128-132 9218435-6 1997 An alternative possibility, that the phosphorylated cyclosome binds directly to a phosphate-binding site of p13(suc1), is supported by the observation that the cyclosome is efficiently eluted from p13(suc1)-Sepharose by phosphate-containing compounds. Sepharose 207-216 H3 histone pseudogene 6 Homo sapiens 108-111 9282913-7 1997 Solubilized PP1bp175 and PP1bp134 interact with PP1C under native conditions, because they both (1) coeluted from size-exclusion and ion-exchange columns; (2) bound to microcystin-LR-Sepharose; and (3) coprecipitated using PP1C antibodies. Sepharose 183-192 protein phosphatase 1, regulatory subunit 9A Rattus norvegicus 12-20 9282913-7 1997 Solubilized PP1bp175 and PP1bp134 interact with PP1C under native conditions, because they both (1) coeluted from size-exclusion and ion-exchange columns; (2) bound to microcystin-LR-Sepharose; and (3) coprecipitated using PP1C antibodies. Sepharose 183-192 protein phosphatase 1, regulatory subunit 9B Rattus norvegicus 25-33 9245707-4 1997 Recombinant murine angiostatin was purified from the culture medium of angiostatin baculovirus-infected insect cells (yield = 1 mg/liter) with a single-step of lysine-Sepharose chromatography. Sepharose 167-176 plasminogen Mus musculus 19-30 9218435-6 1997 An alternative possibility, that the phosphorylated cyclosome binds directly to a phosphate-binding site of p13(suc1), is supported by the observation that the cyclosome is efficiently eluted from p13(suc1)-Sepharose by phosphate-containing compounds. Sepharose 207-216 H3 histone pseudogene 6 Homo sapiens 197-200 9223381-6 1997 From a HT-29 cell lysate, only alphaVbeta6 bound to a fibronectin-Sepharose column. Sepharose 66-75 fibronectin 1 Homo sapiens 54-65 9204878-3 1997 This novel finding of a protein-protein interaction between PP1 and PFK was confirmed by reciprocal experiments in which the binding of PP1 to PFK-agarose was demonstrated. Sepharose 147-154 neuropeptide Y receptor Y4 Rattus norvegicus 60-63 9204878-3 1997 This novel finding of a protein-protein interaction between PP1 and PFK was confirmed by reciprocal experiments in which the binding of PP1 to PFK-agarose was demonstrated. Sepharose 147-154 ATP-dependent 6-phosphofructokinase, muscle type Oryctolagus cuniculus 68-71 9204878-3 1997 This novel finding of a protein-protein interaction between PP1 and PFK was confirmed by reciprocal experiments in which the binding of PP1 to PFK-agarose was demonstrated. Sepharose 147-154 neuropeptide Y receptor Y4 Rattus norvegicus 136-139 9204878-3 1997 This novel finding of a protein-protein interaction between PP1 and PFK was confirmed by reciprocal experiments in which the binding of PP1 to PFK-agarose was demonstrated. Sepharose 147-154 ATP-dependent 6-phosphofructokinase, muscle type Oryctolagus cuniculus 143-146 9204878-4 1997 Elution of PP1 from PFK-agarose was maximal at ca. Sepharose 24-31 neuropeptide Y receptor Y4 Rattus norvegicus 11-14 9204878-4 1997 Elution of PP1 from PFK-agarose was maximal at ca. Sepharose 24-31 ATP-dependent 6-phosphofructokinase, muscle type Oryctolagus cuniculus 20-23 9204878-7 1997 All four known isoforms of PP1 (PP1alpha, PP1gamma1, PP1gamma2, and PP1delta) were shown to bind to PFK-agarose. Sepharose 104-111 neuropeptide Y receptor Y4 Rattus norvegicus 27-30 9204878-7 1997 All four known isoforms of PP1 (PP1alpha, PP1gamma1, PP1gamma2, and PP1delta) were shown to bind to PFK-agarose. Sepharose 104-111 serine/threonine-protein phosphatase PP1-alpha catalytic subunit Oryctolagus cuniculus 32-40 9204878-7 1997 All four known isoforms of PP1 (PP1alpha, PP1gamma1, PP1gamma2, and PP1delta) were shown to bind to PFK-agarose. Sepharose 104-111 ATP-dependent 6-phosphofructokinase, muscle type Oryctolagus cuniculus 100-103 9204883-6 1997 Third, when Triton X-100 extracts of hepatocyte membrane ghosts were chromatographed on Lf-agarose, a 45-kDa polypeptide (p45) was eluted by EGTA. Sepharose 91-98 caspase 1 Rattus norvegicus 122-125 9288226-4 1997 Both aFGF and bFGF, each with a molecular weight of 18 kDa, were identified in PA using heparin-sepharose chromatography and Western blot analysis. Sepharose 96-105 fibroblast growth factor 1 Homo sapiens 5-9 9288226-4 1997 Both aFGF and bFGF, each with a molecular weight of 18 kDa, were identified in PA using heparin-sepharose chromatography and Western blot analysis. Sepharose 96-105 fibroblast growth factor 2 Homo sapiens 14-18 9247717-5 1997 We prepared immobilized BCP bound to AH-Sepharose and examined the activation. Sepharose 40-49 fibroinase Bombyx mori 24-27 9201957-6 1997 Substitutions of negatively charged (Glu195, Glu202, and Asp215) and polar residues (Asn214) of the CRD with alanine but not substitution of the Arg197 with glycine reduced the binding of SP-A to mannose-Sepharose beads and to MSG. Sepharose 204-213 surfactant protein A1 Rattus norvegicus 188-192 9199449-5 1997 rDBP was expressed as a glutathione S-transferase (GST) fusion protein and was isolated from GST by thrombin treatment of the purified fusion protein bound on glutathione agarose beads. Sepharose 171-178 D-box binding PAR bZIP transcription factor Rattus norvegicus 0-4 9232244-1 1997 RNase A, which is routinely added during DNA purification to reduce contaminating RNA, causes shifting of DNA bands in agarose gels. Sepharose 119-126 ribonuclease A family member 1, pancreatic Homo sapiens 0-7 9232244-2 1997 DNA band sizes on agarose gels increase as much as 10%-20% when RNase A is present. Sepharose 18-25 ribonuclease A family member 1, pancreatic Homo sapiens 64-71 9224287-2 1997 METHODS: Vitronectin was removed from fetal bovine serum by heparin-agarose affinity chromatography. Sepharose 68-75 vitronectin Homo sapiens 9-20 9276686-1 1997 A 74-kDa delta/B" subunit was isolated by heparin-Sepharose column chromatography from human erythrocyte protein phosphatase 2A (PP2A) consisting of a 34-kDa catalytic subunit (alpha/C) and 63- and 74-kDa regulatory subunits (beta/A and delta/B") in a ratio of 1:1:1. Sepharose 50-59 protein phosphatase 2 phosphatase activator Homo sapiens 129-133 9262643-2 1997 Chemically synthesized Rat cpn10 was immobilized in a defined orientation to agarose-bound monomeric avidin using a reversible biotinylated affinity label (1), attached to the N alpha-terminal residue. Sepharose 77-84 heat shock protein family E (Hsp10) member 1 Rattus norvegicus 27-32 29512538-1 1997 Myelin basic protein (MBP) from one month old rat brain was purified by CM-52 cellulose chromatography and heparin sepharose chromatography. Sepharose 115-124 myelin basic protein Rattus norvegicus 0-20 9309874-4 1997 The expression patterns of ADH and ALDH isoenzymes were identified by means of agarose isoelectric focusing, and the activities were assayed spectrophotometrically. Sepharose 79-86 alcohol dehydrogenase 1B (class I), beta polypeptide Homo sapiens 27-30 9304300-1 1997 The heterocomplex of glycoproteins E and NS1 of tick-borne encephalitis (TBE) virus was isolated from culture fluid of continuous SPEV (porcine embryo kidney) cells by affinity chromatography on CL4B sepharose with immobilized monoclonal antibodies to TBE virus protein NS1. Sepharose 200-209 influenza virus NS1A binding protein Homo sapiens 41-44 9217011-4 1997 The fusion of the 27 C-terminal amino acids of hEC-SOD to the C-terminal of HCAII (FusCC) resulted in the formation of a monomeric protein, which binds to heparin-Sepharose with approximately the same affinity as the tetrameric hEC-SOD. Sepharose 163-172 superoxide dismutase 3 Homo sapiens 47-54 9217258-3 1997 Fractionation of PARP on Con A-Sepharose revealed that the majority (80 to 90%) of the PARP fraction did not bind to Con A and was composed of the parpA alpha gene product that contains repeats of -Glu-Pro-Pro-Thr- (GPEET-PARP) and that lacks an N-glycosylation site. Sepharose 31-40 collagen type XI alpha 2 chain Homo sapiens 87-91 9217258-3 1997 Fractionation of PARP on Con A-Sepharose revealed that the majority (80 to 90%) of the PARP fraction did not bind to Con A and was composed of the parpA alpha gene product that contains repeats of -Glu-Pro-Pro-Thr- (GPEET-PARP) and that lacks an N-glycosylation site. Sepharose 31-40 collagen type XI alpha 2 chain Homo sapiens 87-91 9200678-11 1997 In another set of experiments, unmodified or poly(ADP-ribosyl)ated pADPRT was immobilized on Sepharose. Sepharose 93-102 poly(ADP-ribose) polymerase 1 Homo sapiens 67-73 9200678-13 1997 Affinity precipitation of HeLa nuclear extracts with poly(ADP-ribosyl)ated pADPRT-Sepharose led to the enrichment of a number of proteins, whereas nuclear proteins bound to the unmodified pADPRT-Sepharose in a smaller extent. Sepharose 82-91 poly(ADP-ribose) polymerase 1 Homo sapiens 75-81 9200678-13 1997 Affinity precipitation of HeLa nuclear extracts with poly(ADP-ribosyl)ated pADPRT-Sepharose led to the enrichment of a number of proteins, whereas nuclear proteins bound to the unmodified pADPRT-Sepharose in a smaller extent. Sepharose 82-91 poly(ADP-ribose) polymerase 1 Homo sapiens 188-194 9200678-13 1997 Affinity precipitation of HeLa nuclear extracts with poly(ADP-ribosyl)ated pADPRT-Sepharose led to the enrichment of a number of proteins, whereas nuclear proteins bound to the unmodified pADPRT-Sepharose in a smaller extent. Sepharose 195-204 poly(ADP-ribose) polymerase 1 Homo sapiens 75-81 9200678-13 1997 Affinity precipitation of HeLa nuclear extracts with poly(ADP-ribosyl)ated pADPRT-Sepharose led to the enrichment of a number of proteins, whereas nuclear proteins bound to the unmodified pADPRT-Sepharose in a smaller extent. Sepharose 195-204 poly(ADP-ribose) polymerase 1 Homo sapiens 188-194 9171112-2 1997 In our assay, the protein is fused to the glutathione-S-transferase and bound to glutathione-Sepharose beads. Sepharose 93-102 glutathione S-transferase kappa 1 Homo sapiens 42-67 9182573-3 1997 In this study, calmodulin-binding proteins were isolated from normal (Hs578Bst) and malignant (MCF-7) human breast cell lines with calmodulin-Sepharose and analyzed by SDS-polyacrylamide gel electrophoresis. Sepharose 142-151 calmodulin 1 Homo sapiens 15-25 9182573-3 1997 In this study, calmodulin-binding proteins were isolated from normal (Hs578Bst) and malignant (MCF-7) human breast cell lines with calmodulin-Sepharose and analyzed by SDS-polyacrylamide gel electrophoresis. Sepharose 142-151 calmodulin 1 Homo sapiens 131-141 9197365-5 1997 Approximately 300 ng of mucin/Ig chimeras absorbed onto 50 microl of packed anti-mouse IgG agarose beads could completely remove cytotoxic human anti-pig antibodies from 1 ml of human AB serum, as estimated in porcine endothelial cell cytotoxicity assays. Sepharose 91-98 LOC100508689 Homo sapiens 24-29 9177183-1 1997 Mammalian selenocysteine-containing thioredoxin reductase (TR) isolated from HeLa cells and from human lung adenocarcinoma cells was separated into two major enzyme species by heparin-agarose affinity chromatography. Sepharose 184-191 peroxiredoxin 5 Homo sapiens 36-57 9177183-1 1997 Mammalian selenocysteine-containing thioredoxin reductase (TR) isolated from HeLa cells and from human lung adenocarcinoma cells was separated into two major enzyme species by heparin-agarose affinity chromatography. Sepharose 184-191 peroxiredoxin 5 Homo sapiens 59-61 9385399-3 1997 The synthesized HBX was assayed for its binding to a glutathione-S-transferase (GST)-pRB fusion protein bound to Sepharose beads. Sepharose 113-122 RB transcriptional corepressor 1 Homo sapiens 85-88 9238529-2 1997 We have developed a procedure for isolating TFR from human placental tissues by affinity chromatography on transferrin-Sepharose. Sepharose 119-128 transferrin receptor Homo sapiens 44-47 9238529-2 1997 We have developed a procedure for isolating TFR from human placental tissues by affinity chromatography on transferrin-Sepharose. Sepharose 119-128 transferrin Homo sapiens 107-118 9276185-2 1997 In 63.0% of the fragments detected by ethidium bromide stained agarose gel electrophoresis (Agarose-EtBr) the sensitivity was insufficient to separate the specific clonal population from the background of normal B cells. Sepharose 63-70 endothelin receptor type B Homo sapiens 100-104 9276185-2 1997 In 63.0% of the fragments detected by ethidium bromide stained agarose gel electrophoresis (Agarose-EtBr) the sensitivity was insufficient to separate the specific clonal population from the background of normal B cells. Sepharose 92-99 endothelin receptor type B Homo sapiens 100-104 9246561-4 1997 When Fab(kappa) was reduced with dithiothreitol (DTT), the Fd fragment in the UF could be separated from the free kappa-chain and the unreduced Fab(kappa) in the BF with a Protein L-Sepharose column. Sepharose 182-191 FA complementation group B Homo sapiens 5-8 9228925-10 1997 Constant production and delivery of proinsulin could be achieved by encapsulating the human insulin cDNA-transfected cells using 5% agarose. Sepharose 132-139 insulin Homo sapiens 36-46 9228925-10 1997 Constant production and delivery of proinsulin could be achieved by encapsulating the human insulin cDNA-transfected cells using 5% agarose. Sepharose 132-139 insulin Homo sapiens 39-46 9271162-1 1997 When human myelogenous leukemic cell lines, HL-60 and ML-1, were exposed to 50 Hz electromagnetic fields (EMFs), nucleosome-sized DNA fragmentation (a biochemical marker of apoptosis) was induced as assessed by agarose gel electrophoresis. Sepharose 211-218 interleukin 17F Homo sapiens 54-58 9154805-4 1997 Ada2 can be precipitated from nuclear extracts by a glutathione S-transferase-tau1 fusion protein coupled to agarose beads, and a direct interaction between Ada2 and tau1c can be shown by using purified proteins. Sepharose 109-116 transcriptional adaptor 2A Homo sapiens 0-4 9179288-4 1997 The conditioned medium, containing recombinant LCAT, was harvested at 24 and 48 h and subjected to chromatography on phenyl-Sepharose and ACA-44 agarose to isolate the recombinant enzyme. Sepharose 145-152 phosphatidylcholine-sterol acyltransferase Mesocricetus auratus 47-51 9213396-2 1997 HIV-1 gp120 binding proteins of the CD4-negative and Gal-C-negative, non-productively infectable human glioblastoma cell line D54 were purified by affinity chromatography over a gp120-conjugated sepharose column and identified by peptide microsequencing. Sepharose 195-204 Envelope surface glycoprotein gp160, precursor Human immunodeficiency virus 1 6-11 9213396-2 1997 HIV-1 gp120 binding proteins of the CD4-negative and Gal-C-negative, non-productively infectable human glioblastoma cell line D54 were purified by affinity chromatography over a gp120-conjugated sepharose column and identified by peptide microsequencing. Sepharose 195-204 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 178-183 9158831-0 1997 A modified sodium dodecyl sulphate-agarose gel/immunoblotting method for apolipoprotein(a) phenotyping using alkaline phosphatase-linked chemiluminescent detection. Sepharose 35-42 lipoprotein(a) Homo sapiens 73-89 9183676-0 1997 Structural colocalisation of type VI collagen and fibronectin in agarose cultured chondrocytes and isolated chondrons extracted from adult canine tibial cartilage. Sepharose 65-72 fibronectin 1 Canis lupus familiaris 50-61 9141532-5 1997 FGF-2 was extracted by heparin-Sepharose affinity chromatography and quantified by specific RIA. Sepharose 31-40 fibroblast growth factor 2 Homo sapiens 0-5 9201618-3 1997 Simultaneous exposure of endothelial cells to VEGF resulted in a dose dependent inhibition of apoptosis when evaluated by: (1) direct counting of cells with morphologic features of apoptosis after acridine orange staining; (2) analysis of DNA fragmentation by (a) agarose gel electrophoresis and (b) fluorescence activated cell sorting (FACS); and (3) viability assays dependent upon mitochondrial function. Sepharose 264-271 vascular endothelial growth factor A Homo sapiens 46-50 9152389-9 1997 Direct interaction between ISIS 2302 and Factor H was demonstrated in a competition assay, where increasing concentrations of ISIS 2302 eluted Factor H from a heparin-sepharose column. Sepharose 167-176 complement factor H Homo sapiens 41-49 9152389-9 1997 Direct interaction between ISIS 2302 and Factor H was demonstrated in a competition assay, where increasing concentrations of ISIS 2302 eluted Factor H from a heparin-sepharose column. Sepharose 167-176 complement factor H Homo sapiens 143-151 12237391-6 1997 The profilin-actin complex was then isolated by affinity chromatography on poly-L-proline-Sepharose, and actin was selectively eluted with a salt wash. Pollen actin was further purified by one cycle of polymerization and depolymerization. Sepharose 90-99 profilin-4 Zea mays 4-12 12237391-6 1997 The profilin-actin complex was then isolated by affinity chromatography on poly-L-proline-Sepharose, and actin was selectively eluted with a salt wash. Pollen actin was further purified by one cycle of polymerization and depolymerization. Sepharose 90-99 actin-7 Zea mays 13-18 9169418-5 1997 Glutathione S-transferase-human transcription factor IIB (TFIIB) fusion protein linked to Sepharose equally coprecipitated the wild-type hVDR and the AF-2 mutants. Sepharose 90-99 cilia and flagella associated protein 20 Homo sapiens 32-56 9169418-5 1997 Glutathione S-transferase-human transcription factor IIB (TFIIB) fusion protein linked to Sepharose equally coprecipitated the wild-type hVDR and the AF-2 mutants. Sepharose 90-99 cilia and flagella associated protein 20 Homo sapiens 58-63 9169418-5 1997 Glutathione S-transferase-human transcription factor IIB (TFIIB) fusion protein linked to Sepharose equally coprecipitated the wild-type hVDR and the AF-2 mutants. Sepharose 90-99 vitamin D receptor Homo sapiens 137-141 9175749-4 1997 The fusion product GST-wALS was purified in a single step on a glutathione-Sepharose column. Sepharose 75-84 glutathione S-transferase Nicotiana tabacum 19-22 9187366-5 1997 This Aplysia CPE is purified on a p-aminobenzoyl-Arg Sepharose affinity column under conditions that selectively purify rat CPE. Sepharose 53-62 carboxypeptidase E Rattus norvegicus 13-16 9164851-7 1997 Treatment of kidney microvillar membranes with phospholipase A2 resulted in the release of membrane dipeptidase in a form that demonstrated electrophoretic and cilastatin-Sepharose binding properties identical to those of the endogenously released form of the enzyme from zymogen granule membranes. Sepharose 171-180 phospholipase A2 group IB Homo sapiens 47-63 9169007-8 1997 Heparin-agarose chromatography of the four antithrombin variants revealed that Gln 96, Gln 135, and Gln 192 variants still displayed the two functional heparin-affinity forms previously observed with the wild-type inhibitor, whereas the Gln 155 variant showed only a single functional high heparin affinity form. Sepharose 8-15 serpin family C member 1 Homo sapiens 43-55 9153406-2 1997 To further examine this interaction, a glutathione S-transferase (GST) fusion protein containing the ligand binding domain of human RXR alpha has been used to copurify the ligand binding domain of human RAR gamma by affinity chromatography over glutathione-agarose. Sepharose 257-264 retinoid X receptor alpha Homo sapiens 132-141 9153406-2 1997 To further examine this interaction, a glutathione S-transferase (GST) fusion protein containing the ligand binding domain of human RXR alpha has been used to copurify the ligand binding domain of human RAR gamma by affinity chromatography over glutathione-agarose. Sepharose 257-264 retinoic acid receptor gamma Homo sapiens 203-212 9168958-3 1997 DNA helicase Q1 in human cell lysates was coprecipitated with bacterially expressed Qip1 and Rch1 fused with glutathione-S-transferase with glutathione Sepharose beads, confirming the interaction between these proteins and DNA helicase Q1. Sepharose 152-161 karyopherin subunit alpha 2 Homo sapiens 93-97 9188815-3 1997 Albumin was separated by chromatography on Blue-Sepharose. Sepharose 48-57 albumin Homo sapiens 0-7 9188826-2 1997 Lysozyme and alpha-lactalbumin were immobilized on an N-hydroxysuccinimide activated agarose support. Sepharose 85-92 lysozyme C, tracheal isozyme Bos taurus 0-8 9188826-2 1997 Lysozyme and alpha-lactalbumin were immobilized on an N-hydroxysuccinimide activated agarose support. Sepharose 85-92 lactalbumin alpha Bos taurus 13-30 9115241-2 1997 Here we show that Rad and Gem bind to calmodulin (CaM)-Sepharose in vitro in a calcium-dependent manner and that Rad can be co-immunoprecipitated with CaM in C2C12 cells. Sepharose 55-64 Ras-related associated with diabetes Mus musculus 18-21 9115241-2 1997 Here we show that Rad and Gem bind to calmodulin (CaM)-Sepharose in vitro in a calcium-dependent manner and that Rad can be co-immunoprecipitated with CaM in C2C12 cells. Sepharose 55-64 GTP binding protein (gene overexpressed in skeletal muscle) Mus musculus 26-29 9115241-2 1997 Here we show that Rad and Gem bind to calmodulin (CaM)-Sepharose in vitro in a calcium-dependent manner and that Rad can be co-immunoprecipitated with CaM in C2C12 cells. Sepharose 55-64 calmodulin 2 Mus musculus 38-48 9115241-2 1997 Here we show that Rad and Gem bind to calmodulin (CaM)-Sepharose in vitro in a calcium-dependent manner and that Rad can be co-immunoprecipitated with CaM in C2C12 cells. Sepharose 55-64 calmodulin 2 Mus musculus 50-53 9115241-2 1997 Here we show that Rad and Gem bind to calmodulin (CaM)-Sepharose in vitro in a calcium-dependent manner and that Rad can be co-immunoprecipitated with CaM in C2C12 cells. Sepharose 55-64 calmodulin 2 Mus musculus 151-154 9115294-6 1997 An endogenous soluble form of this protein, which was designated as placental bikunin, was highly purified from human placenta by sequential kallikrein-Sepharose affinity, gel filtration, and C18 reverse-phase chromatography. Sepharose 152-161 serine peptidase inhibitor, Kunitz type 2 Homo sapiens 68-85 9139874-3 1997 When extracts of the cells were fractionated on columns of heparin-Sepharose, bFGF-like activity was found in extracts from each cell line. Sepharose 67-76 fibroblast growth factor 2 Homo sapiens 78-82 9151897-4 1997 Recombinant MDC was expressed in Chinese hamster ovary cells and purified by heparin-Sepharose chromatography. Sepharose 85-94 C-C motif chemokine ligand 22 Homo sapiens 12-15 9199726-2 1997 Electrophoretic separation of the multiple forms of gamma GT on agarose gel was performed in 20 alcoholic patients (six with cirrhosis and 14 with fatty liver) and the results compared with those obtained in 50 healthy volunteers, 43 patients affected with chronic hepatitis C, 36 patients with posthepatitic cirrhosis and in 52 epileptic patients on long-term anti-epileptic medication. Sepharose 64-71 inactive glutathione hydrolase 2 Homo sapiens 52-60 9176151-6 1997 Association of Rab6p with the atrial granule membranes was also confirmed by immunodiffusion electron microscopy in agarose-embedded atrial granules. Sepharose 116-123 RAB6A, member RAS oncogene family Rattus norvegicus 15-20 9216635-7 1997 Also, the addition of TGF-beta 1 to the breast and prostate cancer cells inhibited colony formation in soft agarose. Sepharose 108-115 transforming growth factor beta 1 Homo sapiens 22-32 9161709-2 1997 The AFP-binding protein (AFPbp) possibly containing the AFP-receptor (rAFP) was isolated from human embryos and human breast cancer tissue using affinity chromatography on an AFP-Sepharose column. Sepharose 179-188 alpha fetoprotein Homo sapiens 4-7 9161709-2 1997 The AFP-binding protein (AFPbp) possibly containing the AFP-receptor (rAFP) was isolated from human embryos and human breast cancer tissue using affinity chromatography on an AFP-Sepharose column. Sepharose 179-188 alpha fetoprotein Homo sapiens 25-28 9161709-2 1997 The AFP-binding protein (AFPbp) possibly containing the AFP-receptor (rAFP) was isolated from human embryos and human breast cancer tissue using affinity chromatography on an AFP-Sepharose column. Sepharose 179-188 alpha fetoprotein Homo sapiens 25-28 9129011-5 1997 The extent of vWF degradation was assayed by electrophoresis in sodium dodecyl sulfate-agarose gels and immunoblotting. Sepharose 87-94 von Willebrand factor Homo sapiens 14-17 9505355-1 1997 The polyclonal antibodies purified by affine chromatography against tyrosyl-tRNA synthetase (TyrRS) immobilized on the column with affigel-sepharose have been obtained from the bovine liver. Sepharose 139-148 tyrosyl-tRNA synthetase 1 Bos taurus 93-98 29512538-1 1997 Myelin basic protein (MBP) from one month old rat brain was purified by CM-52 cellulose chromatography and heparin sepharose chromatography. Sepharose 115-124 myelin basic protein Rattus norvegicus 22-25 9149808-3 1997 Incubation of protein G-Sepharose with non-saturating amounts of 4 Fab preparations, representative of all IgG subclasses, showed that gamma 1, gamma 3 and gamma 4 Fabs adsorbed from 26 to 28.3%, whereas 80% of gamma 2 Fab was left in the supernatant after adsorption. Sepharose 24-33 FA complementation group B Homo sapiens 164-167 9111037-6 1997 Here we demonstrate that heparin binds specifically to recombinant HIV-1 Tat produced as glutathione S-transferase (GST) fusion protein and immobilized on glutathione-agarose beads. Sepharose 167-174 Tat Human immunodeficiency virus 1 73-76 9099704-1 1997 We found that 35S-labeled recombinant human interleukin-1beta (rhIL-1beta) binds phosphatidylinositol-specific phospholipase C-treated human placental alkaline phosphatase, phosphatidylinositol-specific phospholipase C-treated trypanosome surface variant glycoproteins, and urinary uromodulin immobilized on plates or immobilized on CNBr-activated Sepharose 4B. Sepharose 348-357 interleukin 1 beta Homo sapiens 44-61 9108018-1 1997 Mini-chaperones (e.g., a peptide consisting of residues 191-345 of GroEL) that are immobilized on agarose have very efficient chaperoning activity with several proteins that are otherwise recalcitrant to renaturation by conventional methods. Sepharose 98-105 heat shock protein family D (Hsp60) member 1 Homo sapiens 67-72 9175273-5 1997 By using free-solution CE with polyacrylamide-coated capillaries and boric acid-alcohol amine (BA) buffers, ovalbumin of agarose electrophoresis purity can be split into more than twenty peaks at pH 8.0 +/- 0.3 (0.2-0.4 mol/l BA). Sepharose 121-128 ovalbumin (SERPINB14) Gallus gallus 108-117 9251249-2 1997 From 11 of E. coli culture 21 mg of pure PLC delta 1 was obtained by a two-step purification procedure, which includes Ni(2+)-NAT agarose and Mono S cation exchange chromatography. Sepharose 130-137 phospholipase C delta 1 Homo sapiens 41-52 9092516-4 1997 Soluble heparin inhibits adhesion, and Ang itself binds tightly to heparin-Sepharose. Sepharose 75-84 angiogenin Homo sapiens 39-42 9092516-7 1997 Mutations of these residues, and of Arg-70 as well, decrease both the affinity of Ang for heparin-Sepharose and the capacity of Ang to support cell adhesion. Sepharose 98-107 angiogenin Homo sapiens 82-85 9115748-8 1997 The growth-promoting activity from U937 cells that bound to heparin-sepharose was inhibited by a neutralizing antibody to human HB-EGF. Sepharose 68-77 heparin binding EGF like growth factor Homo sapiens 128-134 9092566-5 1997 GRK4alpha kinase activity was inhibited by Ca2+/calmodulin (CaM) (IC50 = 80 nM), and a direct interaction between GRK4alpha and Ca2+/CaM was revealed using CaM-conjugated Sepharose 4B. Sepharose 171-183 G protein-coupled receptor kinase 4 Homo sapiens 0-9 9092566-5 1997 GRK4alpha kinase activity was inhibited by Ca2+/calmodulin (CaM) (IC50 = 80 nM), and a direct interaction between GRK4alpha and Ca2+/CaM was revealed using CaM-conjugated Sepharose 4B. Sepharose 171-183 calmodulin 3 Homo sapiens 60-63 9092566-5 1997 GRK4alpha kinase activity was inhibited by Ca2+/calmodulin (CaM) (IC50 = 80 nM), and a direct interaction between GRK4alpha and Ca2+/CaM was revealed using CaM-conjugated Sepharose 4B. Sepharose 171-183 G protein-coupled receptor kinase 4 Homo sapiens 114-123 9092566-5 1997 GRK4alpha kinase activity was inhibited by Ca2+/calmodulin (CaM) (IC50 = 80 nM), and a direct interaction between GRK4alpha and Ca2+/CaM was revealed using CaM-conjugated Sepharose 4B. Sepharose 171-183 calmodulin 3 Homo sapiens 133-136 9092566-5 1997 GRK4alpha kinase activity was inhibited by Ca2+/calmodulin (CaM) (IC50 = 80 nM), and a direct interaction between GRK4alpha and Ca2+/CaM was revealed using CaM-conjugated Sepharose 4B. Sepharose 171-183 calmodulin 3 Homo sapiens 133-136 9105676-4 1997 Previously, we have demonstrated that N1E-115 cells possess two distinct subpopulations of AT2 receptors, defined as peak I and peak III receptors, that can be separated by heparin-sepharose chromatography. Sepharose 181-190 angiotensin II receptor type 2 Homo sapiens 91-94 9126381-1 1997 The complex formed between 32P-labeled (dT)15 and a hexahistidine (6-His)-tagged anti-single-stranded DNA (ssDNA) Fab, DNA-1, was trapped by addition of nickel-chelating nitrilotriacetic acid (Ni-NTA) agarose that led to efficient separation of bound ligand from free. Sepharose 201-208 FA complementation group B Homo sapiens 114-117 9163329-5 1997 The recombinant SP-D was purified on a nickel column and then on a maltose-agarose column. Sepharose 75-82 surfactant protein D Homo sapiens 16-20 9147055-2 1997 Both secretory IgA subclasses bound to ABL-Sepharose and the IgA2 subclass (which contains only N-glycans) was recovered with a high degree of purity when NH4OH was used as eluent. Sepharose 43-52 CD79a molecule Homo sapiens 15-18 9146940-7 1997 Specificity controls included omission of the primary antibody and substitution of the primary antibody with non-immune normal rabbit IgG or with Charcot-Leyden crystal protein-Sepharose-absorbed primary antibody. Sepharose 177-186 Charcot-Leyden crystal galectin Homo sapiens 146-176 9149391-2 1997 The lectin binds to Sepharose (an agarose polymer) in a calcium-dependent manner. Sepharose 20-29 lectin Oncorhynchus mykiss 4-10 9149391-2 1997 The lectin binds to Sepharose (an agarose polymer) in a calcium-dependent manner. Sepharose 34-41 lectin Oncorhynchus mykiss 4-10 9147056-2 1997 Analysis of amino acid sequence revealed that p180 has a strong homology to the macrophage mannose receptor (MMR), which was corroborated by the observation that p180 reacted with polyclonal anti-alveolar MMR antibody and mannosyl-BSA-agarose. Sepharose 235-242 protein phosphatase 1, regulatory subunit 9A Rattus norvegicus 46-50 9147056-2 1997 Analysis of amino acid sequence revealed that p180 has a strong homology to the macrophage mannose receptor (MMR), which was corroborated by the observation that p180 reacted with polyclonal anti-alveolar MMR antibody and mannosyl-BSA-agarose. Sepharose 235-242 protein phosphatase 1, regulatory subunit 9A Rattus norvegicus 162-166 9111501-6 1997 Binding levels were not identical; however, hIgE-Dp2A bound significantly better to the mite-extract sepharose. Sepharose 101-110 immunoglobulin heavy constant epsilon Homo sapiens 44-48 9111501-8 1997 The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Sepharose 76-85 immunoglobulin heavy constant epsilon Homo sapiens 48-51 9111501-8 1997 The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Sepharose 76-85 immunoglobulin heavy constant epsilon Homo sapiens 72-75 9111501-8 1997 The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Sepharose 76-85 immunoglobulin heavy constant epsilon Homo sapiens 72-75 9111501-8 1997 The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Sepharose 76-85 immunoglobulin heavy constant epsilon Homo sapiens 72-75 9111501-8 1997 The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Sepharose 158-167 immunoglobulin heavy constant epsilon Homo sapiens 48-51 9136969-4 1997 Depletion of the IgG by absorption of plasma with protein G-Sepharose in vitro resulted in normalization of the thrombin time and reptilase time. Sepharose 60-69 coagulation factor II, thrombin Homo sapiens 112-120 9111501-8 1997 The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Sepharose 158-167 immunoglobulin heavy constant epsilon Homo sapiens 72-75 9111501-8 1997 The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Sepharose 158-167 immunoglobulin heavy constant epsilon Homo sapiens 72-75 9111501-8 1997 The less efficient binding of the detector anti-IgE in case of the anti-IgE sepharose is likely to be because of the occupation of epitopes of the IgE by the sepharose-bound anti-IgE. Sepharose 158-167 immunoglobulin heavy constant epsilon Homo sapiens 72-75 9126601-6 1997 Furthermore, OPN coupled to Sepharose was capable of binding the alpha v beta 3 integrin from a detergent extract of endothelial cells. Sepharose 28-37 secreted phosphoprotein 1 Bos taurus 13-16 9126612-1 1997 In the present work we describe a procedure for the purification of human pregnancy zone protein (PZP) from pooled late pregnancy plasma by using hydrophobic interaction chromatography (HIC) on a phenyl-Sepharose column. Sepharose 203-212 PZP alpha-2-macroglobulin like Homo sapiens 74-96 9126612-1 1997 In the present work we describe a procedure for the purification of human pregnancy zone protein (PZP) from pooled late pregnancy plasma by using hydrophobic interaction chromatography (HIC) on a phenyl-Sepharose column. Sepharose 203-212 PZP alpha-2-macroglobulin like Homo sapiens 98-101 9126612-7 1997 In addition, considering the different hydrophobic properties exhibited by native PZP and PZP-protease complexes, HIC on phenyl-Sepharose column could also be used for separating both conformational states of PZP. Sepharose 128-137 PZP alpha-2-macroglobulin like Homo sapiens 82-85 9126612-7 1997 In addition, considering the different hydrophobic properties exhibited by native PZP and PZP-protease complexes, HIC on phenyl-Sepharose column could also be used for separating both conformational states of PZP. Sepharose 128-137 PZP alpha-2-macroglobulin like Homo sapiens 90-93 9126612-7 1997 In addition, considering the different hydrophobic properties exhibited by native PZP and PZP-protease complexes, HIC on phenyl-Sepharose column could also be used for separating both conformational states of PZP. Sepharose 128-137 PZP alpha-2-macroglobulin like Homo sapiens 90-93 9167445-3 1997 The anti-CCK F (ab")2 fragment was immobilized to the Sepharose 4B gel activated BrCN and packed to column. Sepharose 54-63 cholecystokinin Rattus norvegicus 9-12 9096214-9 1997 These results were confirmed through affinity chromatography, where UvsX and UvsY bound to Endo VII, immobilized on a NHS-activated Sepharose matrix. Sepharose 132-141 recombination mediator protein UvsY Escherichia phage T4 77-81 9178346-1 1997 A soluble 65582.9 Da (in MALDI-TOF) angiotensin converting (ACE)-like enzyme has been purified by a captopril-Sepharose affinity column chromatography from the mollusk Mytilus edulis. Sepharose 110-119 angiotensin I converting enzyme Homo sapiens 60-63 9054425-4 1997 Like other FGF-2 proteins, bacterially produced rainbow trout FGF-2 protein binds tightly to heparin-Sepharose and also promotes the proliferation of fibroblast cells. Sepharose 101-110 fibroblast growth factor 2 Homo sapiens 62-67 9054394-2 1997 The stability of the complex was indicated by the copurification of MLCKase and MLCPase activities through multiple steps that included myofibril preparation, gel filtration chromatography, cation (SP-Sepharose BB) and anion (Q-Sepharose FF) exchange chromatography, and affinity purification on calmodulin and on thiophosphorylated regulatory light chain columns. Sepharose 201-210 myosin light chain kinase Homo sapiens 68-75 9054394-2 1997 The stability of the complex was indicated by the copurification of MLCKase and MLCPase activities through multiple steps that included myofibril preparation, gel filtration chromatography, cation (SP-Sepharose BB) and anion (Q-Sepharose FF) exchange chromatography, and affinity purification on calmodulin and on thiophosphorylated regulatory light chain columns. Sepharose 228-237 myosin light chain kinase Homo sapiens 68-75 9119086-2 1997 The cytosolic domain of the human mitochondrial protein import receptor, hTom20, has been expressed as a fusion protein with glutathione S-transferase (GST) in bacteria and the purified protein immobilized on Sepharose beads. Sepharose 209-218 translocase of outer mitochondrial membrane 20 Homo sapiens 73-79 9062127-3 1997 N- and C-terminal truncations were also placed in the same vector, allowing the expression and purification of glutathione S-transferase (GST)-PDE fusion proteins using glutathione-Sepharose. Sepharose 181-190 aldehyde dehydrogenase 7 family member A1 Homo sapiens 143-146 9124530-8 1997 The elution profile of this activity from gelatin-Sepharose 4B was similar to matrix metalloproteinase 2 (MMP-2, a 72-kDa matrixin with a 62-kDa mature form), and the dimethyl sulfoxide eluant from these columns contained MMP-2 immunoreactivity. Sepharose 50-59 matrix metallopeptidase 2 Rattus norvegicus 222-227 9049034-4 1997 Examination of whether sperm binding to PNA-, UEA-1-, WGA-, Con A-, or PSA-coated agarose microbeads reflected sperm binding to biological ZP and correlated with in vitro fertilization rates showed that only binding to WGA-coated microbeads showed significant positive and negative predictive values when compared to IVF rates in 2 x 2 contingency. Sepharose 82-89 aminopeptidase puromycin sensitive Homo sapiens 71-74 9074423-9 1997 Plating of FACS-sorted sub-populations of CD34+ marrow cells in both serum-free agarose and methylcellulose assays demonstrated that most CFC-Mk are generated from CD34+ cells that are CD45RA- and CD71+, approximately half of which are CD41+. Sepharose 80-87 CD34 molecule Homo sapiens 42-46 9060459-7 1997 Recombinant MCP-4 was expressed in mammalian cells and purified by heparin-Sepharose chromatography. Sepharose 75-84 mast cell protease 4 Mus musculus 12-17 9075127-14 1997 Analysis of plasma vWF multimeric pattern by SDS-agarose gel electrophoresis disclosed in four IgAN patients abnormally large vWF multimers that were not documented in PGN subjects. Sepharose 49-56 von Willebrand factor Homo sapiens 19-22 9056490-3 1997 With repeated cycles of freezing and thawing as a first step, the purification of the recombinant ch-DHFR to homogeneity requires only one further step, gel filtration on a Sephadex G-75 column with 85-90% enzyme recovery, two to three times higher than that obtained with the commonly used affinity chromatography on a methotrexate-Sepharose column. Sepharose 333-342 dihydrofolate reductase Escherichia coli 101-105 9132063-1 1997 The Arabidopsis G alpha subunit, GP alpha1, was expressed within Escherichia coli by co-transformation with the expression vector and the dnaY gene which encodes tRNA(Arg)(AGA/AGG) Isolation of the recombinant GP alpha1 in a highly pure form could be achieved by a combination of anion exchange and dye affinity chromatography or by a single step affinity procedure via chromatography on 4-amino-anilido-GTP agarose. Sepharose 408-415 G protein alpha subunit 1 Arabidopsis thaliana 4-42 9148252-2 1997 Agarose electrophoresis of the amplified products showed reverse transcription polymerase chain reaction (RT-PCR) products of the expected size for both TH and AADC. Sepharose 0-7 dopa decarboxylase Homo sapiens 160-164 9068694-5 1997 Human recombinant acidic fibroblast growth factor (hraFGF) imbided in agarose was topically administered at the craniectomy site. Sepharose 70-77 fibroblast growth factor 1 Rattus norvegicus 18-49 9074491-4 1997 The mannan-binding protein fraction of bovine serum that eluted with 100 mM mannose from a mannan-Sepharose column was analyzed under reducing conditions by SDS-PAGE. Sepharose 98-107 mannose binding lectin 2 Bos taurus 4-26 9020107-4 1997 Results from affinity chromatography on m7GTP-agarose demonstrated that AII-induced phosphorylation of 4E-BP1 promotes its dissociation from eIF4E in target cells. Sepharose 46-53 angiotensinogen Homo sapiens 72-75 9020107-4 1997 Results from affinity chromatography on m7GTP-agarose demonstrated that AII-induced phosphorylation of 4E-BP1 promotes its dissociation from eIF4E in target cells. Sepharose 46-53 eukaryotic translation initiation factor 4E binding protein 1 Homo sapiens 103-109 9020107-4 1997 Results from affinity chromatography on m7GTP-agarose demonstrated that AII-induced phosphorylation of 4E-BP1 promotes its dissociation from eIF4E in target cells. Sepharose 46-53 eukaryotic translation initiation factor 4E Homo sapiens 141-146 9009306-6 1997 In this study, by using affinity chromatography with biotinylated HLf and streptavidin-agarose, we identified a 70-kDa lactoferrin-binding protein (Lbp) from outer membrane proteins of H. pylori. Sepharose 87-94 lipopolysaccharide binding protein Homo sapiens 148-151 9135700-5 1997 The Fab fragment was separated by Protein A-Sepharose after papain hydrolysis of TSAb-IgG, then separated into two Fab(kappa) and Fab(lambda) forms by Protein L-Sepharose. Sepharose 44-53 FA complementation group B Homo sapiens 4-7 9135700-5 1997 The Fab fragment was separated by Protein A-Sepharose after papain hydrolysis of TSAb-IgG, then separated into two Fab(kappa) and Fab(lambda) forms by Protein L-Sepharose. Sepharose 161-170 FA complementation group B Homo sapiens 4-7 9026427-8 1997 Agarose gel electrophoresis of the PCR products yielded a single band corresponding to the 290-bp HGF product for both TIC and GC. Sepharose 0-7 hepatocyte growth factor Rattus norvegicus 98-101 9225113-8 1997 Uterine GCP-2 exhibited high affinity to heparin agarose, a characteristic shared by all alpha chemokines. Sepharose 49-56 C-X-C motif chemokine 6 Bos taurus 8-13 21153117-8 1997 Uterine GCP-2 exhibited high affinity to heparin agarose, a characteristic shared by all alpha chemokines. Sepharose 49-56 C-X-C motif chemokine 6 Bos taurus 8-13 9343926-4 1997 When these fusion proteins (or GST), immobilized on glutathione-agarose beads were incubated with [35S] methionine labelled cell extracts, multiple proteins which interact specifically with SH3 domain of Hck were detected by SDS-PAGE followed by autoradiography. Sepharose 64-71 glutathione S-transferase kappa 1 Homo sapiens 31-34 9343926-4 1997 When these fusion proteins (or GST), immobilized on glutathione-agarose beads were incubated with [35S] methionine labelled cell extracts, multiple proteins which interact specifically with SH3 domain of Hck were detected by SDS-PAGE followed by autoradiography. Sepharose 64-71 HCK proto-oncogene, Src family tyrosine kinase Homo sapiens 204-207 9343926-12 1997 Hck was detected in the eluate of WGA-Sepharose column, suggesting that it interacts with WGA binding glycoprotein (s). Sepharose 38-47 HCK proto-oncogene, Src family tyrosine kinase Homo sapiens 0-3 9343936-4 1997 The 800 bp cDNA fragment encoding a region (90%) of alpha 2-3 sialyltransferase (SAT-3) catalytic domain from ECB has been expressed as a glutathione S-transferase (GST) soluble fusion protein (62 kDa) in E. coli and purified over glutathione-agarose affinity matrix. Sepharose 243-250 ST3 beta-galactoside alpha-2,3-sialyltransferase 4 Homo sapiens 81-86 9023203-5 1997 VirB1* was purified from the supernatant of vir-induced cells by ammonium sulfate precipitation and Q-Sepharose chromatography. Sepharose 102-111 type IV secretion system lytic transglycosylase VirB1 Agrobacterium tumefaciens 0-5 9071984-12 1997 This suggests that the 44-46 kDa IGFBP-3 in serum was degraded to yield a 31 kDa fragment, while any endogenous IGFBP-5 could not pass out of the agarose. Sepharose 146-153 insulin-like growth factor binding protein 3 Rattus norvegicus 33-40 9116502-9 1997 To isolate eIF-4E binding proteins, recombinant eIF-4E was linked to agarose beads and incubated with cell lysates. Sepharose 69-76 eukaryotic translation initiation factor 4E Homo sapiens 48-54 9203626-10 1997 Using specific antibodies and immunoadsorption to protein A/Sepharose columns, we found that G protein isotypes G(alpha q/11), G(alpha i3), and G(alpha s), and effector enzymes PLC-beta1, PLC-gamma2 and PLA-2 were associated with the antagonist-labeled receptor in the 400-kDa fraction. Sepharose 60-69 phospholipase C beta 1 Rattus norvegicus 177-186 8999954-5 1997 Wheat germ agglutinin-Sepharose affinity chromatography also readily detected terminal GlcNAc residues on subpopulations of ER purified from calf uterus, from human breast cancer cells (MCF-7), or from mER produced by in vitro translation. Sepharose 22-31 MER proto-oncogene tyrosine kinase Mus musculus 124-126 9037257-1 1997 Previously, two antiestrogens estradiol derivatives (3 and 4) bearing the basic side chain of tamoxifen were shown to impede the binding of the estrogen receptor (ER) to calmodulin (CaM)-Sepharose. Sepharose 187-196 estrogen receptor 1 Homo sapiens 144-161 9037257-1 1997 Previously, two antiestrogens estradiol derivatives (3 and 4) bearing the basic side chain of tamoxifen were shown to impede the binding of the estrogen receptor (ER) to calmodulin (CaM)-Sepharose. Sepharose 187-196 estrogen receptor 1 Homo sapiens 163-165 9037257-1 1997 Previously, two antiestrogens estradiol derivatives (3 and 4) bearing the basic side chain of tamoxifen were shown to impede the binding of the estrogen receptor (ER) to calmodulin (CaM)-Sepharose. Sepharose 187-196 calmodulin 1 Homo sapiens 170-180 9037257-1 1997 Previously, two antiestrogens estradiol derivatives (3 and 4) bearing the basic side chain of tamoxifen were shown to impede the binding of the estrogen receptor (ER) to calmodulin (CaM)-Sepharose. Sepharose 187-196 calmodulin 1 Homo sapiens 182-185 8999954-5 1997 Wheat germ agglutinin-Sepharose affinity chromatography also readily detected terminal GlcNAc residues on subpopulations of ER purified from calf uterus, from human breast cancer cells (MCF-7), or from mER produced by in vitro translation. Sepharose 22-31 MER proto-oncogene tyrosine kinase Mus musculus 202-205 9030741-8 1997 When expressed as a recombinant fusion protein in bacteria, APP-1 bound specifically to poly(A)-Sepharose. Sepharose 96-105 poly(A) binding protein cytoplasmic 4 Homo sapiens 60-65 9020891-6 1997 The binding of GST P1-1 to antibody adsorbed to Protein A-Sepharose was also prevented by both 0.1 mM GSH and N-ethylmaleimide treatment. Sepharose 58-67 glutathione S-transferase pi 1 Homo sapiens 15-23 9027364-14 1997 DNA fragmentation in PC-3(AR)2 cells after 3 or 6 days of DHT treatment was demonstrated by agarose gel electrophoresis, further indicating the cell death was apoptotic. Sepharose 92-99 androgen receptor Homo sapiens 26-28 8995429-3 1997 The interaction between RF-C and proliferating cell nuclear antigen (PCNA) was studied with PCNA-agarose beads. Sepharose 97-104 proliferating cell nuclear antigen Saccharomyces cerevisiae S288C 33-67 8995429-3 1997 The interaction between RF-C and proliferating cell nuclear antigen (PCNA) was studied with PCNA-agarose beads. Sepharose 97-104 proliferating cell nuclear antigen Saccharomyces cerevisiae S288C 69-73 8995429-3 1997 The interaction between RF-C and proliferating cell nuclear antigen (PCNA) was studied with PCNA-agarose beads. Sepharose 97-104 proliferating cell nuclear antigen Saccharomyces cerevisiae S288C 92-96 8995429-4 1997 Binding of RF-C to PCNA-agarose beads is negligible in buffers containing 0.3 M NaCl. Sepharose 24-31 proliferating cell nuclear antigen Saccharomyces cerevisiae S288C 19-23 9003440-1 1997 Hepsin, a putative cell-surface serine proteinase, has been isolated from the microsomal membranes of rat liver and purified to homogeneity by hydroxyapatite, DEAE-Sepharose, and benzamidine-Sepharose chromatography. Sepharose 164-173 hepsin Rattus norvegicus 0-6 9025988-4 1997 CD34+ cells were plated in agarose with different combinations of cytokines. Sepharose 27-34 CD34 molecule Homo sapiens 0-4 18629959-10 1997 When the ligand H-TPR-OH was immobilized via its N-terminal on agarose, at a concentration of 14 micromol/g gel, it produced the most effective affinity chromatography adsorbent. Sepharose 63-70 nucleoprotein TPR Bos taurus 18-21 9360709-1 1997 UDP-glucose:solanidine glucosyltransferase and UDP-galactose:solanidine galactosyltransferase from cytosol of potato sprouts were partially separated by Sephadex G-100 and Q-Sepharose chromatographies, proving the existence of different glycosylation systems in biosynthesis of alpha-chaconine and alpha-solanine. Sepharose 174-183 solanidine UDP-glucose glucosyltransferase 1 Solanum tuberosum 47-93 9192073-7 1997 Recombinant apoE3(1-183) was isolated by a combination of heparin-Sepharose chromatography and reverse-phase HPLC. Sepharose 66-75 apolipoprotein E Homo sapiens 12-17 9417344-5 1997 Since October 1992, we carried out 229 immunoadsorptions (IA) with specific columns containing anti-Lp(a) antibodies covalently bound to sepharose. Sepharose 137-146 lipoprotein(a) Homo sapiens 100-105 9610889-8 1997 The IGF-I did not bind to heparin-sepharose, while CGF bound to it and was eluted with 0.6M NaCl from heparin-sepharose columns. Sepharose 110-119 insulin like growth factor 1 Homo sapiens 4-9 9054465-10 1997 Calmodulin-agarose beads bound NHE-1 extracted from SHR cells to a lesser extent than that from WKY cells. Sepharose 11-18 calmodulin 1 Rattus norvegicus 0-10 9054465-10 1997 Calmodulin-agarose beads bound NHE-1 extracted from SHR cells to a lesser extent than that from WKY cells. Sepharose 11-18 solute carrier family 9 member A1 Rattus norvegicus 31-36 8977402-5 1997 The recombinant (rec) 20 alpha HSD expressed as glutathione-S-transferase-20 alpha HSD fusion protein was purified from bacterial lysates by affinity binding to glutathione-Sepharose beads followed by thrombin digestion, whereas the rec enzyme expressed in baculovirus-insect cell system was purified to apparent homogeneity by ion exchange chromatography, followed by dye affinity chromatographies. Sepharose 173-182 aldo-keto reductase family 1, member C3 Rattus norvegicus 22-34 9022035-5 1997 Sepharose 4B gel chromatography of postnuclear supernatants from Nonidet-P40-solubilized antigen (Ag)- or pervanadate-activated RBL cells revealed extensive changes in the size of complexes formed by Lyn and Syk kinases and other cellular components. Sepharose 0-9 LYN proto-oncogene, Src family tyrosine kinase Rattus norvegicus 200-203 9022035-5 1997 Sepharose 4B gel chromatography of postnuclear supernatants from Nonidet-P40-solubilized antigen (Ag)- or pervanadate-activated RBL cells revealed extensive changes in the size of complexes formed by Lyn and Syk kinases and other cellular components. Sepharose 0-9 spleen associated tyrosine kinase Rattus norvegicus 208-211 9610889-9 1997 Heparin-sepharose 0.2M NaCl fractions of cementum extracts contained IGF-I migrating with Mr 7,500, but its mobility was not affected by N-glycosidase treatment. Sepharose 8-17 insulin like growth factor 1 Homo sapiens 69-74 8989571-2 1997 In an agarose drop migration assay PDGF and bFGF stimulated migration while GGF-2 had no effect. Sepharose 6-13 fibroblast growth factor 2 Homo sapiens 44-48 9093876-2 1997 Anti-gp41-antibodies affinity-purified from sera of HIV-1-infected individuals (stage A) using rsgp41-Sepharose column could recognize human IFN-alpha in ELISA, but no antibody against IFN-alpha was detected if immunoglobulins were prepared in the same way from pooled HIV-negative serum. Sepharose 102-111 interferon alpha 1 Homo sapiens 141-150 9211750-4 1997 The NKR-P1 and the CD94-containing complexes were independent of each other and both very large, as judged by Sepharose 4B gel chromatography. Sepharose 110-119 killer cell lectin like receptor B1 Homo sapiens 4-10 9211750-4 1997 The NKR-P1 and the CD94-containing complexes were independent of each other and both very large, as judged by Sepharose 4B gel chromatography. Sepharose 110-119 killer cell lectin like receptor D1 Homo sapiens 19-23 9403355-2 1997 The aim of this study was to verify if analgesic drugs, such as morphine and ketorolac, may affect the c-fos protooncogene expression by using a method highly sensitive and specific, based on transformation of activated c-fos specific mRNA in cDNA (reverse transcription), its amplification (PCR) and final visualization by electrophoresis on agarose gel. Sepharose 343-350 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 103-108 9403355-2 1997 The aim of this study was to verify if analgesic drugs, such as morphine and ketorolac, may affect the c-fos protooncogene expression by using a method highly sensitive and specific, based on transformation of activated c-fos specific mRNA in cDNA (reverse transcription), its amplification (PCR) and final visualization by electrophoresis on agarose gel. Sepharose 343-350 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 220-225 9034196-3 1997 Based on their mass composition and electrophoretic mobility in agarose gel, the released apoA-I-containing particles are similar to previously described pre beta-HDL particles known as primary acceptors of peripheral cholesterol. Sepharose 64-71 apolipoprotein A1 Sus scrofa 90-96 9869862-20 1997 In conclusion, the present study indicates that the agarose-based microencapsulated islets can function in large diabetic animals, resulting in the independence of exogenous insulin therapy for prolonged periods without the need for immunosuppression. Sepharose 52-59 insulin Canis lupus familiaris 174-181 9034202-7 1997 LDL binding to LPL containing heparin-agarose was also disrupted by the amino-terminal antibodies to apoB. Sepharose 38-45 lipoprotein lipase Homo sapiens 15-18 9343823-5 1997 The agarose gel coupled with reactive red 120 (RR120), another triazine dye with similar characteristics, also retained both Env proteins, and the bound Env proteins could be eluted in a similar manner. Sepharose 4-11 endogenous retrovirus group K member 20 Homo sapiens 125-128 9034202-7 1997 LDL binding to LPL containing heparin-agarose was also disrupted by the amino-terminal antibodies to apoB. Sepharose 38-45 apolipoprotein B Homo sapiens 101-105 21533339-5 1997 Anchorage-independent growth in hard agarose was enhanced only in bFGF-transfected nonmetastatic C-10 cells which, subsequent to the transfection, also expressed high levels of collagenase IV/gelatinase A activity. Sepharose 37-44 fibroblast growth factor 2 Mus musculus 66-70 21533365-5 1997 This growth factor(s) bound to heparin Sepharose (HS) beads and its effects on cell proliferation were partially blocked by a neutralizing bFGF antibody. Sepharose 39-48 fibroblast growth factor 2 Mus musculus 139-143 9343823-5 1997 The agarose gel coupled with reactive red 120 (RR120), another triazine dye with similar characteristics, also retained both Env proteins, and the bound Env proteins could be eluted in a similar manner. Sepharose 4-11 endogenous retrovirus group K member 20 Homo sapiens 153-156 9031461-3 1997 To verify this hypothesis we have purified IgG autoantibodies to beta 2-GPI from plasma of 6 patients with antiphospholipid antibody syndrome, by means of agarose-immobilized human beta 2-GPI. Sepharose 155-162 apolipoprotein H Homo sapiens 65-75 9008393-2 1997 After purification by glutathione-Sepharose chromatography, the glutathione S-transferase moiety was cleaved off and the resulting PAL enzyme analyzed. Sepharose 34-43 glutathione S-transferase Zea mays 64-89 12215771-6 1997 In addition it could bind on the heparin-Sepharose affinity chromatography column as Apo-E. Sepharose 41-50 apolipoprotein E Homo sapiens 85-90 12215802-3 1997 The cell-modified VLDL migrated faster than the controls on agarose electrophoregram and the SDS-PAGE of the apolipoproteins showed that apo B(100) of VLDL was degraded to fragments without distinct bands, while apo E was essentially kept intact. Sepharose 60-67 CD320 antigen Mus musculus 18-22 9031461-3 1997 To verify this hypothesis we have purified IgG autoantibodies to beta 2-GPI from plasma of 6 patients with antiphospholipid antibody syndrome, by means of agarose-immobilized human beta 2-GPI. Sepharose 155-162 apolipoprotein H Homo sapiens 181-191 9116643-2 1996 Binding ability was related to human immunoglobulin G because bovine lactoferrin binding proteins were isolated by extraction of cell surface associated proteins with distilled water, applied on IgG-Sepharose and nickel sulphate chelate affinity chromatography. Sepharose 199-208 lactotransferrin Bos taurus 69-80 21781743-8 1996 A combination of isoelectric focusing-agarose gel electrophoresis (IEF-AGE) and synchrotron radiation X-ray fluorescence (SR-XRF) analysis revealed that Mn-SOD rather than Cu,Zn-SOD underwent modification. Sepharose 38-45 superoxide dismutase 2, mitochondrial Mus musculus 153-159 8943314-4 1996 Furthermore, AChRs affinity-isolated from C2 myotubes using alpha-bungarotoxin-Sepharose were specifically associated with Src and Fyn and had tyrosine-phosphorylated beta subunits. Sepharose 79-88 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 123-126 8971189-4 1996 Thioredoxin-transfected MCF-7 cells showed unaltered monolayer growth on plastic surfaces compared with vector alone-transfected cells, but exhibited severalfold increased colony formation in soft agarose. Sepharose 197-204 thioredoxin Homo sapiens 0-11 8985151-1 1996 Human placental acid sphingomyelinase (ASM) was purified by sequential chromatography on Con A-Sepharose, octyl-Sepharose and Matrex gel red A. Sepharose 95-104 sphingomyelin phosphodiesterase 1 Homo sapiens 39-42 8943314-4 1996 Furthermore, AChRs affinity-isolated from C2 myotubes using alpha-bungarotoxin-Sepharose were specifically associated with Src and Fyn and had tyrosine-phosphorylated beta subunits. Sepharose 79-88 FYN proto-oncogene, Src family tyrosine kinase Homo sapiens 131-134 8952474-6 1996 To demonstrate the plant heterocomplex, we add purified mammalian p23, preadsorbed with the JJ3 antibody to protein A-Sepharose, to wheat germ lysate and allow ATP-dependent formation of an animal p23. Sepharose 118-127 prostaglandin E synthase 3 Homo sapiens 66-69 8986639-1 1996 A new type of major aminopeptidase was purified from bovine brain by ammonium sulfate fractionation and TMAE-fractogel (anion exchange), arginine-Sepharose 4B, Sephadex G-150, and Sephadex G-100 column chromatography. Sepharose 146-155 carboxypeptidase Q Homo sapiens 20-34 9222425-2 1996 vWF antigen (vWFAg)-like protein was obtained by gel filtration of the concentrate on Sepharose 4B. Sepharose 86-98 von Willebrand factor Homo sapiens 0-3 8973615-4 1996 Binding of C1-INH to paraproteins, transferred to Immobilon after agarose gel electrophoresis, was detectable in five of seven M-components associated with AAE. Sepharose 66-73 serpin family G member 1 Homo sapiens 11-17 8940380-4 1996 The heparin Sepharose purified material degraded IGFBP-5 into 22-, 17-, and 16-kDa fragments. Sepharose 12-21 insulin like growth factor binding protein 5 Homo sapiens 49-56 8940380-9 1996 When this same pool of protease activity that had been eluted from heparin Sepharose was applied to an alpha1 antichymotrypsin peptide affinity column, the protease activity that bound to the column was inhibited by 3,4 dichloroisocoumain, but was not inhibited by TIMP-1. Sepharose 75-84 serpin family A member 3 Homo sapiens 103-126 8940380-9 1996 When this same pool of protease activity that had been eluted from heparin Sepharose was applied to an alpha1 antichymotrypsin peptide affinity column, the protease activity that bound to the column was inhibited by 3,4 dichloroisocoumain, but was not inhibited by TIMP-1. Sepharose 75-84 TIMP metallopeptidase inhibitor 1 Homo sapiens 265-271 8971542-5 1996 HLA-DPB1 exon 2 was amplified, recovered from agarose gel, and used as a template for subsequent low-stringency (30 degrees C) amplification cycles (LS-PCR) in the presence of a single primer. Sepharose 46-53 major histocompatibility complex, class II, DP beta 1 Homo sapiens 0-8 9237234-2 1996 The primary aim of this study was to use a commercially available in-vitro transcription and translation system to produce immobilized recombinant human ZP3 (rhuZP3) on agarose beads. Sepharose 169-176 zona pellucida glycoprotein 3 Homo sapiens 153-156 8954887-7 1996 Recombinant TP2 was purified from the soluble extract of E. coli using nickel-agarose and heparin-agarose chromatography and was shown to be identical to native rat TP2 as revealed by immunoblotting with anti-rat TP2 antibodies and radioactive 65Zn-blotting. Sepharose 78-85 transition protein 2 Rattus norvegicus 12-15 8954887-7 1996 Recombinant TP2 was purified from the soluble extract of E. coli using nickel-agarose and heparin-agarose chromatography and was shown to be identical to native rat TP2 as revealed by immunoblotting with anti-rat TP2 antibodies and radioactive 65Zn-blotting. Sepharose 98-105 transition protein 2 Rattus norvegicus 12-15 10168548-5 1996 Since October 1992, 186 immunoadsorption treatments have been carried out weekly with Sepharose-coupled anti-Lp(a)-columns. Sepharose 86-95 lipoprotein(a) Homo sapiens 109-114 8940072-2 1996 The recombinant polypeptide was purified from cytosol by sequential tandem affinity chromatographies employing ATP-agarose and calmodulin-Sepharose stationary phases. Sepharose 138-147 LOC100759184 Cricetulus griseus 127-137 8942655-3 1996 N-His (D381E) ICE was expressed in Escherichia coli and purified by nickel-chelating Sepharose and size-exclusion chromatography (SEC). Sepharose 85-94 caspase 1 Homo sapiens 14-17 8960098-3 1996 We report a new method for determination of this genotype in which an allele-specific restriction enzyme site is introduced into an Fc gamma RIIA PCR product from genomic DNA, and polymorphism assignment is determined by restriction enzyme digestion followed by agarose gel electrophoresis. Sepharose 262-269 Fc gamma receptor IIa Homo sapiens 132-145 8939938-3 1996 During purification of factors required for protein folding in the cytosol, we found that the ATP-agarose binding activity of the yeast Hsp70 Ssa1p in postribosomal supernatants was inhibited by NEM. Sepharose 98-105 Hsp70 family ATPase SSA1 Saccharomyces cerevisiae S288C 142-147 8939938-4 1996 We also found that completely removing nucleotides from purified Ssa1p rendered its ATP-agarose binding activity, ATPase activity, and post-translational translocation-stimulating activity sensitive to NEM. Sepharose 88-95 Hsp70 family ATPase SSA1 Saccharomyces cerevisiae S288C 65-70 8914924-9 1996 Immunoprecipitation of this PTP with Syp-antibody coupled to protein A-agarose confirmed the vanadate-induced decrease in SHPTP2 activity. Sepharose 71-78 protein tyrosine phosphatase, non-receptor type 1 Rattus norvegicus 28-31 8916921-2 1996 Here we describe methods for the purification of an insulin-stimulated insulin receptor serine kinase from human placenta and rat liver by sequential chromatography of solubilized membranes on wheat germ agglutinin-agarose, Mono Q, phenyl-Superose, and Superose 12. Sepharose 215-222 insulin Homo sapiens 52-59 8920919-4 1996 After co-amplification, both products of target ER cDNA and internal standard were separated on agarose gel by electrophoresis and transferred onto a membrane filter. Sepharose 96-103 estrogen receptor 1 Homo sapiens 48-50 8910504-10 1996 A direct interaction between GRK5 and Ca2+/CaM was revealed using CaM-conjugated Sepharose 4B. Sepharose 81-90 G protein-coupled receptor kinase 5 Homo sapiens 29-33 8918934-2 1996 Bacterially expressed rat NTF2 exists in solution as dimers and, when bound to Sepharose beads, is able to interact specifically with both the Ras-like GTPase Ran, and the xFxFG repeat containing domains of nucleoporins p62 (vertebrate) and Nsp1p (yeast). Sepharose 79-88 nuclear transport factor 2 Rattus norvegicus 26-30 8918934-2 1996 Bacterially expressed rat NTF2 exists in solution as dimers and, when bound to Sepharose beads, is able to interact specifically with both the Ras-like GTPase Ran, and the xFxFG repeat containing domains of nucleoporins p62 (vertebrate) and Nsp1p (yeast). Sepharose 79-88 RAN, member RAS oncogene family Rattus norvegicus 159-162 9206331-10 1996 The mobility of the amplification products on 4% agarose gels indicates that the leucine-rich amelogenin polypeptide (LRAP) is expressed in the opossum and that the major amelogenin is larger than its homologue in the mouse. Sepharose 49-56 amelogenin, X-linked Mus musculus 81-116 8982248-1 1996 An experimental set-up for estimating a) cellular migration under agarose and b) response to chemoattractant gradients built up in the agarose was used in order to explore the behavior of adherent interleukin-2 (IL-2)-activated natural killer (A-NK) cells on cell culture plastic and after coating with extracellular matrix (ECM) constituents. Sepharose 135-142 interleukin 2 Mus musculus 197-210 8950040-2 1996 Mannose 6-phosphate receptor (MPR) proteins designated as MPR 300 and MPR 46 have earlier been purified from some mammals on phosphomannan coupled to cyanogen bromide activated Sepharose. Sepharose 177-186 mannose-6-phosphate receptor, cation dependent Homo sapiens 70-76 8985594-3 1996 Calmodulin-binding proteins present in this nuclear subfraction were purified by chromatography using first a DEAE-Sephacel column and subsequently a calmodulin-Sepharose column. Sepharose 161-170 calmodulin 1 Rattus norvegicus 0-10 8932996-7 1996 Agarose gel electrophoresis of DNA extracted from cells exposed to IL-1 alpha and agonistic anti-Fas showed internucleosomal DNA fragmentation, a hallmark of apoptotic cell death. Sepharose 0-7 interleukin 1 alpha Mus musculus 67-77 8937850-4 1996 The fusion protein, His-P-PST, was isolated from the bacterial cytosol in a single affinity chromatography step, using a Ni2+ agarose column. Sepharose 126-133 sulfotransferase family 1A member 1 Homo sapiens 24-29 8910336-6 1996 Using antibodies to Lck and to the cyclin-dependent serine kinase, Cdc2, as well as the cyclin-dependent kinase affinity resin, Suc1-agarose, we detected a stable interaction between Lck and Cdc2. Sepharose 133-140 LCK proto-oncogene, Src family tyrosine kinase Homo sapiens 183-186 8910336-6 1996 Using antibodies to Lck and to the cyclin-dependent serine kinase, Cdc2, as well as the cyclin-dependent kinase affinity resin, Suc1-agarose, we detected a stable interaction between Lck and Cdc2. Sepharose 133-140 cyclin dependent kinase 1 Homo sapiens 191-195 8982850-2 1996 The dissociated MetRS complex fraction was purified by gel filtration followed by tRNA-Sepharose chromatography using partially purified tRNA(met) in Method I, and by hydrophobic interaction chromatography in Method II. Sepharose 87-96 methionyl-tRNA synthetase 1 Rattus norvegicus 16-21 9004081-9 1996 Agarose gel electrophoresis of the products of SCFR reverse transcription-polymerase chain reaction derived from each tumour had the molecular weight predicted for canine SCFR cDNA on the basis of the mouse and human counterparts. Sepharose 0-7 KIT proto-oncogene, receptor tyrosine kinase Canis lupus familiaris 47-51 9004081-9 1996 Agarose gel electrophoresis of the products of SCFR reverse transcription-polymerase chain reaction derived from each tumour had the molecular weight predicted for canine SCFR cDNA on the basis of the mouse and human counterparts. Sepharose 0-7 KIT proto-oncogene, receptor tyrosine kinase Canis lupus familiaris 171-175 8946317-0 1996 Tryptophan hydroxylase: purification by affinity chromatography on calmodulin-sepharose. Sepharose 78-87 calmodulin 1 Rattus norvegicus 67-77 8946317-3 1996 Tryptophan hydroxylase binds to calmodulin-Sepharose in the presence of calcium and is eluted with either EGTA or calmodulin itself, but not with tryptophan. Sepharose 43-52 calmodulin 1 Rattus norvegicus 32-42 8959022-3 1996 ssCRE-BP was purified from the mouse cerebellum by a combination of chromatography on DNA affinity agarose and Mono Q HR. Sepharose 99-106 purine rich element binding protein A Mus musculus 0-8 8917617-4 1996 C1q (identified from its behaviour on SDS/PAGE, immunoreactivity with C1q-specific monoclonal antibodies and N-terminal sequencing data) was purified from serum by AMP-Sepharose chromatography. Sepharose 168-177 complement C1q A chain Homo sapiens 0-3 8917617-5 1996 The binding of C1q to AMP-Sepharose was inhibited by adenine nucleotides. Sepharose 26-35 complement C1q A chain Homo sapiens 15-18 8905463-2 1996 The apo D concentrations of samples of the serum, plasma and CSF were directly proportional to the migration distances of the corresponding zones of immunoprecipitates developed during electrophoresis in glass capillaries filled with antibody-containing agarose gel. Sepharose 254-261 apolipoprotein D Homo sapiens 4-9 8912663-5 1996 Since MBL was found to bind to underivatized Sepharose 4B, the MBL-MASP complex was purified using Sepharose 4B and protease inhibitors were included to purify the complex with MASP in its proenzyme form. Sepharose 45-54 mannose binding lectin 2 Homo sapiens 6-9 8912663-5 1996 Since MBL was found to bind to underivatized Sepharose 4B, the MBL-MASP complex was purified using Sepharose 4B and protease inhibitors were included to purify the complex with MASP in its proenzyme form. Sepharose 45-54 mannose binding lectin 2 Homo sapiens 63-66 8912663-5 1996 Since MBL was found to bind to underivatized Sepharose 4B, the MBL-MASP complex was purified using Sepharose 4B and protease inhibitors were included to purify the complex with MASP in its proenzyme form. Sepharose 45-54 MBL associated serine protease 1 Homo sapiens 67-71 8912663-5 1996 Since MBL was found to bind to underivatized Sepharose 4B, the MBL-MASP complex was purified using Sepharose 4B and protease inhibitors were included to purify the complex with MASP in its proenzyme form. Sepharose 45-57 mannose binding lectin 2 Homo sapiens 6-9 8912663-5 1996 Since MBL was found to bind to underivatized Sepharose 4B, the MBL-MASP complex was purified using Sepharose 4B and protease inhibitors were included to purify the complex with MASP in its proenzyme form. Sepharose 45-57 mannose binding lectin 2 Homo sapiens 63-66 8912663-5 1996 Since MBL was found to bind to underivatized Sepharose 4B, the MBL-MASP complex was purified using Sepharose 4B and protease inhibitors were included to purify the complex with MASP in its proenzyme form. Sepharose 45-57 MBL associated serine protease 1 Homo sapiens 67-71 8855944-11 1996 The cross-linked dimers of V5D and N-Gal-1 were isolated and were similar to native lectin in both hemagglutinating activity and high-affinity binding to lactosyl-Sepharose. Sepharose 163-172 galectin 1 Homo sapiens 37-42 8875978-4 1996 Tumour cell growth in vitro and colony formation in soft agarose were greatly inhibited by Ad.E1A(+) transduction in HER-2/neu-overexpressing lung cancer cell lines. Sepharose 57-64 erb-b2 receptor tyrosine kinase 2 Mus musculus 117-122 8875978-4 1996 Tumour cell growth in vitro and colony formation in soft agarose were greatly inhibited by Ad.E1A(+) transduction in HER-2/neu-overexpressing lung cancer cell lines. Sepharose 57-64 erb-b2 receptor tyrosine kinase 2 Mus musculus 123-126 8898021-9 1996 Megalin-Sepharose columns bound 125I-labeled BSA as well as 125I-RSA, the binding was inhibited by RAP and EDTA, and analysis of the eluate revealed the bound tracer to be albumin. Sepharose 8-17 LDL receptor related protein 2 Rattus norvegicus 0-7 8921160-4 1996 The insert (545 bp) of the Drosophila clone 2(8) containing 66 copies of GATA repeats was used to prepare the sequence-specific DNA-Sepharose affinity column. Sepharose 132-141 Glutamyl-tRNA amidotransferase, subunit A Drosophila melanogaster 73-77 8839849-6 1996 When detergent extracts of platelets were subjected to fibulin-1-Sepharose affinity chromatography, the integrin alpha IIb beta 3 was selected. Sepharose 65-74 fibulin 1 Homo sapiens 55-64 8816821-3 1996 Tyrosine phosphorylation of NR2B was measured in two ways: binding of antiphosphotyrosine antibodies (PY20) to glycoprotein(s) of 180 kDa (GP180) purified on Con A-Sepharose and binding of anti-NR2B antibodies to tyrosine-phosphorylated proteins purified on PY20-agarose. Sepharose 164-173 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 28-32 8909940-2 1996 Genetic typing of C4A and B alleles is routinely carried out by high-voltage agarose gel electrophoresis. Sepharose 77-84 complement C4A (Rodgers blood group) Homo sapiens 18-27 8816429-8 1996 Using hapten-conjugated-BSA (DNS-BSA) as a model Ag we show that the Ab response elicited by anti-DNS-IgG3-IL-2-bound DNS-BSA-Sepharose injected into mice is increased over that of DNS-BSA-Sepharose or anti-DNS-IgG3-bound DNS-BSA-Sepharose. Sepharose 189-198 Immunoglobulin heavy constant gamma 3 Mus musculus 102-106 8816429-8 1996 Using hapten-conjugated-BSA (DNS-BSA) as a model Ag we show that the Ab response elicited by anti-DNS-IgG3-IL-2-bound DNS-BSA-Sepharose injected into mice is increased over that of DNS-BSA-Sepharose or anti-DNS-IgG3-bound DNS-BSA-Sepharose. Sepharose 189-198 interleukin 2 Mus musculus 107-111 8798650-3 1996 The human homologs of the MCM2 (BM28), Mis5, Cdc21, and CDC47 proteins were tightly bound to a histone-Sepharose column and purified to near homogeneity, whereas the P1/MCM3 and CDC46/MCM5 proteins passed through. Sepharose 103-112 minichromosome maintenance complex component 2 Homo sapiens 26-30 8798650-3 1996 The human homologs of the MCM2 (BM28), Mis5, Cdc21, and CDC47 proteins were tightly bound to a histone-Sepharose column and purified to near homogeneity, whereas the P1/MCM3 and CDC46/MCM5 proteins passed through. Sepharose 103-112 minichromosome maintenance complex component 2 Homo sapiens 32-36 8798650-3 1996 The human homologs of the MCM2 (BM28), Mis5, Cdc21, and CDC47 proteins were tightly bound to a histone-Sepharose column and purified to near homogeneity, whereas the P1/MCM3 and CDC46/MCM5 proteins passed through. Sepharose 103-112 minichromosome maintenance complex component 6 Homo sapiens 39-43 8798650-3 1996 The human homologs of the MCM2 (BM28), Mis5, Cdc21, and CDC47 proteins were tightly bound to a histone-Sepharose column and purified to near homogeneity, whereas the P1/MCM3 and CDC46/MCM5 proteins passed through. Sepharose 103-112 minichromosome maintenance complex component 7 Homo sapiens 56-61 8943308-6 1996 Solubilized 78-kDa EPOR bound to purified, genetically engineered JAK2 better than the 62-76-kDa receptor proteins, and additional phosphorylation of tyrosine residues further increased the binding of the 78-kDa EPOR to JAK2-agarose beads. Sepharose 225-232 erythropoietin receptor Mus musculus 19-23 8816821-3 1996 Tyrosine phosphorylation of NR2B was measured in two ways: binding of antiphosphotyrosine antibodies (PY20) to glycoprotein(s) of 180 kDa (GP180) purified on Con A-Sepharose and binding of anti-NR2B antibodies to tyrosine-phosphorylated proteins purified on PY20-agarose. Sepharose 263-270 glutamate ionotropic receptor NMDA type subunit 2B Rattus norvegicus 28-32 8880136-2 1996 DNA fragmentation of measles virus-infected THP-1 cells was demonstrated by DNA agarose gel electrophoresis as well as by DNA fragmentation ELISA. Sepharose 80-87 GLI family zinc finger 2 Homo sapiens 44-49 8805661-7 1996 Expression of baboon CR1b yielded a membrane protein that reacted with an anti-CR1 mAb, was identical in size to baboon E-CR, and, like baboon E-CR, could bind baboon C3 linked to activated thiol-Sepharose (C3i-ATS), but not human C3i-ATS. Sepharose 196-205 LOW QUALITY PROTEIN: complement receptor type 1 Papio anubis 21-24 8814225-7 1996 The kinetic data revealed that the maize histone deacetylase HD1-B peak after partial purification by Q-Sepharose chromatography was heterogeneous and consisted of two histone binding sites that differed significantly in their affinity for purified core histones. Sepharose 104-113 histone deacetylase Zea mays 41-60 8814225-7 1996 The kinetic data revealed that the maize histone deacetylase HD1-B peak after partial purification by Q-Sepharose chromatography was heterogeneous and consisted of two histone binding sites that differed significantly in their affinity for purified core histones. Sepharose 104-113 histone deacetylase 108 Zea mays 61-66 8809039-4 1996 The CRD of SP-D, without the neck region peptide, was also expressed and shown to behave as a monomer that showed a very weak affinity for maltose-agarose, LPSs and phospholipids. Sepharose 147-154 surfactant protein D Homo sapiens 11-15 8924055-8 1996 Oxygen radical-induced damage on apolipoprotein-B100 was evaluated by acrylamide and agarose gel electrophoresis. Sepharose 85-92 apolipoprotein B Homo sapiens 33-52 8894274-2 1996 These results were confirmed by affinity chromatography experiments demonstrating that S100A1 and S100B bound to phosphoglucomutase-Sepharose in a calcium-dependent manner. Sepharose 132-141 S100 calcium binding protein A1 Homo sapiens 87-93 8894274-2 1996 These results were confirmed by affinity chromatography experiments demonstrating that S100A1 and S100B bound to phosphoglucomutase-Sepharose in a calcium-dependent manner. Sepharose 132-141 S100 calcium binding protein B Homo sapiens 98-103 8894274-3 1996 In the reverse experiment, phosphoglucomutase bound to S100A1 and S100B-Sepharose in a calcium-dependent manner. Sepharose 72-81 S100 calcium binding protein B Homo sapiens 66-71 8905256-4 1996 For DNA of approximately 1, 2 and 3 kbp, D(diff) and D(disp) were measured in agarose gel electrophoresis (1.0% SeaKem GTG). Sepharose 78-85 gamma-glutamyltransferase 1 Homo sapiens 119-122 8814268-1 1996 Stimulation in vitro of murine splenic B cells by lipopolysaccharide, anti-kappa Sepharose, anti-CD40 or allo-reactive T helper cells all up-regulated CD21 and CD23 surface expression. Sepharose 81-90 complement receptor 2 Mus musculus 151-155 8814268-1 1996 Stimulation in vitro of murine splenic B cells by lipopolysaccharide, anti-kappa Sepharose, anti-CD40 or allo-reactive T helper cells all up-regulated CD21 and CD23 surface expression. Sepharose 81-90 Fc receptor, IgE, low affinity II, alpha polypeptide Mus musculus 160-164 8814268-3 1996 However, anti-CD40-stimulated B cells showed increased proliferation in the presence of anti-CD21 antibodies coupled to Sepharose; co-stimulation via CD21 also induced differentiation to immunoglobulin secretion in a fraction of anti-CD40-stimulated B cells. Sepharose 120-129 CD40 antigen Mus musculus 14-18 8814268-3 1996 However, anti-CD40-stimulated B cells showed increased proliferation in the presence of anti-CD21 antibodies coupled to Sepharose; co-stimulation via CD21 also induced differentiation to immunoglobulin secretion in a fraction of anti-CD40-stimulated B cells. Sepharose 120-129 complement receptor 2 Mus musculus 93-97 8709225-6 1996 Using the pre-S protein fused to glutathione S-transferase and immobilized on Sepharose beads, we have now identified an additional binding protein with a size of 120 kDa (p120). Sepharose 78-87 catenin delta 1 Homo sapiens 172-176 8816429-8 1996 Using hapten-conjugated-BSA (DNS-BSA) as a model Ag we show that the Ab response elicited by anti-DNS-IgG3-IL-2-bound DNS-BSA-Sepharose injected into mice is increased over that of DNS-BSA-Sepharose or anti-DNS-IgG3-bound DNS-BSA-Sepharose. Sepharose 126-135 Immunoglobulin heavy constant gamma 3 Mus musculus 102-106 8816429-8 1996 Using hapten-conjugated-BSA (DNS-BSA) as a model Ag we show that the Ab response elicited by anti-DNS-IgG3-IL-2-bound DNS-BSA-Sepharose injected into mice is increased over that of DNS-BSA-Sepharose or anti-DNS-IgG3-bound DNS-BSA-Sepharose. Sepharose 126-135 interleukin 2 Mus musculus 107-111 8816429-8 1996 Using hapten-conjugated-BSA (DNS-BSA) as a model Ag we show that the Ab response elicited by anti-DNS-IgG3-IL-2-bound DNS-BSA-Sepharose injected into mice is increased over that of DNS-BSA-Sepharose or anti-DNS-IgG3-bound DNS-BSA-Sepharose. Sepharose 126-135 Immunoglobulin heavy constant gamma 3 Mus musculus 211-215 8816429-8 1996 Using hapten-conjugated-BSA (DNS-BSA) as a model Ag we show that the Ab response elicited by anti-DNS-IgG3-IL-2-bound DNS-BSA-Sepharose injected into mice is increased over that of DNS-BSA-Sepharose or anti-DNS-IgG3-bound DNS-BSA-Sepharose. Sepharose 189-198 Immunoglobulin heavy constant gamma 3 Mus musculus 102-106 8816429-8 1996 Using hapten-conjugated-BSA (DNS-BSA) as a model Ag we show that the Ab response elicited by anti-DNS-IgG3-IL-2-bound DNS-BSA-Sepharose injected into mice is increased over that of DNS-BSA-Sepharose or anti-DNS-IgG3-bound DNS-BSA-Sepharose. Sepharose 189-198 interleukin 2 Mus musculus 107-111 8812848-5 1996 Fusion apo(a) Kr V was isolated from cytoplasmic extracts and purified by amylose-agarose affinity chromatography by eluting with 10 mM maltose. Sepharose 82-89 lipoprotein(a) Homo sapiens 7-13 9239682-6 1996 Heparanase (endo-beta-glucuronidase) was isolated and purified by ammonium sulphate precipitation followed by sequential chromatographies on carboxymethyl-, heparin- and ConA-Sepharose columns. Sepharose 175-184 heparanase Homo sapiens 0-10 8812854-5 1996 Hirudin, the most specific thrombin inhibitor, and hirudin-based peptides were covalently immobilized to Sepharose, thus creating thrombin-specific affinity gels that immediately absorb the thrombin generated from the activation mixture. Sepharose 105-114 coagulation factor II, thrombin Homo sapiens 130-138 8812854-5 1996 Hirudin, the most specific thrombin inhibitor, and hirudin-based peptides were covalently immobilized to Sepharose, thus creating thrombin-specific affinity gels that immediately absorb the thrombin generated from the activation mixture. Sepharose 105-114 coagulation factor II, thrombin Homo sapiens 130-138 8883269-8 1996 First, we used fibrin monomers coupled to Sepharose to which thrombin and unfractionated heparin (UFH) were bound. Sepharose 42-51 coagulation factor II, thrombin Homo sapiens 61-69 9185264-5 1996 GM-CSF was entirely adsorbed to concanavalin A-Sepharose 4B affinity chromatography, and was sensitive to pronase digestion, indicating its glycoprotein nature. Sepharose 47-56 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 0-6 8883269-9 1996 Factor XI activation by thrombin was the same in the presence of fibrin-Sepharose or control-Sepharose. Sepharose 72-81 coagulation factor II, thrombin Homo sapiens 24-32 8883269-9 1996 Factor XI activation by thrombin was the same in the presence of fibrin-Sepharose or control-Sepharose. Sepharose 93-102 coagulation factor II, thrombin Homo sapiens 24-32 8702861-3 1996 Purification of this protein by sequential column chromatographies revealed an 18-kDa doublet, which was identified as calmodulin by Western blotting, calcium-dependent precipitation with W-7 agarose beads, and reconstitution of calcium-mediated phospholipase A2 inhibition with authentic homogeneous calmodulin. Sepharose 192-199 calmodulin 1 Homo sapiens 119-129 8752130-3 1996 Actin and its binding proteins were then isolated by affinity chromatography on DNase I-Sepharose and by one- and two-dimensional polyacrylamide gel electrophoresis. Sepharose 88-97 actin, beta Gallus gallus 0-5 8702861-5 1996 Moreover, ternary complex affinity chromatography with calmodulin-Sepharose demonstrated that inhibition of myocardial phospholipase A2 activity by calmodulin resulted from the direct interaction of calmodulin with the myocardial phospholipase A2 catalytic complex. Sepharose 66-75 calmodulin 1 Homo sapiens 55-65 8702861-5 1996 Moreover, ternary complex affinity chromatography with calmodulin-Sepharose demonstrated that inhibition of myocardial phospholipase A2 activity by calmodulin resulted from the direct interaction of calmodulin with the myocardial phospholipase A2 catalytic complex. Sepharose 66-75 phospholipase A2 group IB Homo sapiens 119-135 8702861-5 1996 Moreover, ternary complex affinity chromatography with calmodulin-Sepharose demonstrated that inhibition of myocardial phospholipase A2 activity by calmodulin resulted from the direct interaction of calmodulin with the myocardial phospholipase A2 catalytic complex. Sepharose 66-75 calmodulin 1 Homo sapiens 148-158 8702861-5 1996 Moreover, ternary complex affinity chromatography with calmodulin-Sepharose demonstrated that inhibition of myocardial phospholipase A2 activity by calmodulin resulted from the direct interaction of calmodulin with the myocardial phospholipase A2 catalytic complex. Sepharose 66-75 calmodulin 1 Homo sapiens 148-158 8702861-5 1996 Moreover, ternary complex affinity chromatography with calmodulin-Sepharose demonstrated that inhibition of myocardial phospholipase A2 activity by calmodulin resulted from the direct interaction of calmodulin with the myocardial phospholipase A2 catalytic complex. Sepharose 66-75 phospholipase A2 group IB Homo sapiens 230-246 8756702-6 1996 Recombinant Msi4p with an N-terminal polyhistidine leader was purified on a Ni(2+)-Sepharose column, followed by gel filtration and ion exchange chromatography. Sepharose 83-92 GTPase-activating protein MRS6 Saccharomyces cerevisiae S288C 12-17 8702753-3 1996 Furthermore, p62 bound to ubiquitin-conjugated Sepharose beads in vitro and was efficiently competed by soluble ubiquitin. Sepharose 47-56 sequestosome 1 Homo sapiens 13-16 8695871-7 1996 Polymerase chain reaction products from DNA mixtures containing as low as 0.5 ng of a DRB4-positive DNA control in 1.0 microgram of a DRB4-negative DNA sample (1:2 x 10(3) dilution) showed a visible DRB4 band in agarose gels stained with ethidium bromide. Sepharose 212-219 major histocompatibility complex, class II, DR beta 4 Homo sapiens 86-90 8695871-7 1996 Polymerase chain reaction products from DNA mixtures containing as low as 0.5 ng of a DRB4-positive DNA control in 1.0 microgram of a DRB4-negative DNA sample (1:2 x 10(3) dilution) showed a visible DRB4 band in agarose gels stained with ethidium bromide. Sepharose 212-219 major histocompatibility complex, class II, DR beta 4 Homo sapiens 134-138 8695871-7 1996 Polymerase chain reaction products from DNA mixtures containing as low as 0.5 ng of a DRB4-positive DNA control in 1.0 microgram of a DRB4-negative DNA sample (1:2 x 10(3) dilution) showed a visible DRB4 band in agarose gels stained with ethidium bromide. Sepharose 212-219 major histocompatibility complex, class II, DR beta 4 Homo sapiens 134-138 8702680-5 1996 GFRP is dissociated from the GTP agarose-bound complex with 0.2 NaCl, a concentration of salt which also effectively blocks the tetrahydrobiopterin-dependent inhibitory activity of GFRP. Sepharose 33-40 GTP cyclohydrolase I feedback regulator Rattus norvegicus 0-4 8702680-5 1996 GFRP is dissociated from the GTP agarose-bound complex with 0.2 NaCl, a concentration of salt which also effectively blocks the tetrahydrobiopterin-dependent inhibitory activity of GFRP. Sepharose 33-40 GTP cyclohydrolase I feedback regulator Rattus norvegicus 181-185 8702680-6 1996 GTP cyclohydrolase I is then eluted from the GTP-agarose column with GTP. Sepharose 49-56 GTP cyclohydrolase 1 Rattus norvegicus 0-20 8756800-3 1996 IgE-independent HRF due to chemokines was removed from mononuclear cell supernatants with heparin-Sepharose. Sepharose 98-107 tumor protein, translationally-controlled 1 Homo sapiens 16-19 8806718-0 1996 Purification of rat liver xanthine oxidase and xanthine dehydrogenase by affinity chromatography on benzamidine-sepharose. Sepharose 112-121 xanthine dehydrogenase Rattus norvegicus 47-69 8695857-9 1996 mRNA from purified SS clones but not mRNA from SS total PBMC was positive for AF-1 in an agarose gel and/or after hybridization. Sepharose 89-96 interferon gamma receptor 2 Homo sapiens 78-82 8806718-1 1996 The oxidase form of xanthine dehydrogenase (XO; EC 1.1.3.22) has been purified approximately 200-fold from rat liver extracts using a three-step process of heat treatment, ammonium sulfate precipitation, and chromatography on benzamidine-Sepharose. Sepharose 238-247 xanthine dehydrogenase Rattus norvegicus 20-42 8774702-2 1996 By the direct expression of a synthetic gene encoding human eIF-4E as the soluble form in Escherichia coli and the application on a 7-methylguanosine-5"-triphosphate-Sepharose 4B cap affinity column, pure recombinant eIF-4E was prepared; the optimum pH for the binding of the mRNA cap was 7.5. Sepharose 166-178 eukaryotic translation initiation factor 4E Homo sapiens 60-66 8811836-3 1996 A series of chromatographic steps (DEAE-Sepharose, Sephacryl S-200 and Activated Thiol Sepharose 4B) generated a soluble PAP activity purified to near homogeneity with a total active yield of 6.6% The enzyme displayed a native molecular mass of approximately 23,700 Da, which compares well with that value obtained under denaturing conditions via SDS-PAGE (24,000 Da), suggesting that the enzyme exists as a monomer. Sepharose 40-49 PDGFA associated protein 1 Homo sapiens 121-124 8881765-4 1996 The antigen-biding activity of IgM NAb increased after isolation of the serum immunoglobulins on a Staphylococcus Protein A (SPA)-Sepharose column. Sepharose 130-139 pulmonary surfactant-associated protein A Sus scrofa 125-128 8764609-8 1996 The soluble 5"-nucleotidase showed an elution profile on AMP-Sepharose affinity chromatography or on Mono Q ion-exchange chromatography different from that of the brain ectoenzyme. Sepharose 61-70 5' nucleotidase, ecto Rattus norvegicus 12-27 8806438-6 1996 Using a unique affinity purification protocol (Ugi-Sepharose) and anti-UDG2 antibodies, we have identified a physical interaction between the cyclin-like uracil-DNA glycosylase and PCNA in extracts derived from HeLa cells. Sepharose 51-60 proliferating cell nuclear antigen Homo sapiens 181-185 8806438-10 1996 We show that UDG2 and PCNA are coprecipitated using anti-PCNA antibodies and anti-UDG2 antibodies as well as Ugi-Sepharose. Sepharose 113-122 cyclin O Homo sapiens 13-17 8806438-10 1996 We show that UDG2 and PCNA are coprecipitated using anti-PCNA antibodies and anti-UDG2 antibodies as well as Ugi-Sepharose. Sepharose 113-122 proliferating cell nuclear antigen Homo sapiens 22-26 8864964-12 1996 Finally, VLDL from HuCIITg and MoCIIITg mice showed decreased binding to heparin-Sepharose. Sepharose 81-90 CD320 antigen Mus musculus 9-13 8878798-5 1996 PLC isozymes in tissue extracts of the lung were partially purified by successive chromatographic steps on heparin-sepharose CL-6B conventional and TSKgel heparin-5PW HPLC columns and their activities were assayed. Sepharose 115-124 heparan sulfate proteoglycan 2 Homo sapiens 0-3 8797889-0 1996 Selective enhancement by tumor necrosis factor-alpha of vascular permeability of new blood vessels induced with agarose hydrogel-entrapped Meth-A fibrosarcoma cells. Sepharose 112-119 tumor necrosis factor Mus musculus 25-52 8797889-2 1996 In this article, we report that the new blood vessels induced with agarose-encapsulated tumor cells have the same sensitivity to tumor necrosis factor-alpha (TNF-alpha) as the original solid-tumor vessels. Sepharose 67-74 tumor necrosis factor Mus musculus 129-156 8797889-2 1996 In this article, we report that the new blood vessels induced with agarose-encapsulated tumor cells have the same sensitivity to tumor necrosis factor-alpha (TNF-alpha) as the original solid-tumor vessels. Sepharose 67-74 tumor necrosis factor Mus musculus 158-167 8875777-2 1996 Rabbit alpha 2M was purified with ultracentrifugation, gel filtration on Sepharose 6B and ion exchange chromatography on DEAE-Sephacel. Sepharose 73-82 LOW QUALITY PROTEIN: alpha-2-macroglobulin Oryctolagus cuniculus 7-15 8864949-3 1996 Lp[a] was further purified using a lysine-Sepharose affinity column and Lp[a]- obtained by incubating Lp[a] with dithiothreitol. Sepharose 42-51 lipoprotein(a) Homo sapiens 0-4 8844414-5 1996 We were able to identify four distinct subspecies of Lp(a) showing different affinity to epsilon-amino groups of lysine-Sepharose, simply by modifying molarity and pH of the eluents; the fraction obtained in highly purified state represented the major form and could be eluted with 0.5 M sodium phosphate buffer (pH 4.4). Sepharose 120-129 lipoprotein(a) Homo sapiens 53-58 8693544-4 1996 Specific IgM depletion was accomplished with an immunoabsorption column containing sheep anti-human IgM (mu-chain specific) conjugated to Sepharose beads. Sepharose 138-147 Ig kappa chain Sus scrofa 9-12 8844414-4 1996 We present here a procedure for Lp(a) purification based on sequential elutions after lysine-Sepharose affinity chromatography. Sepharose 93-102 lipoprotein(a) Homo sapiens 32-36 8760249-4 1996 ABP was purified from rat renal proximal tubular cell brush-border membrane by affinity chromatography with rat serum albumin-Sepharose. Sepharose 126-135 sex hormone binding globulin Rattus norvegicus 0-3 8874867-4 1996 Reteplase and alteplase bound completely to a heparin-agarose column and eluted respectively at 0.39 M and 0.60 M in a NaCl gradient. Sepharose 54-61 plasminogen activator, tissue type Homo sapiens 0-9 8855402-0 1996 Resolution of apolipoprotein B repeat unit position variants on agarose, denaturing, and native polyacrylamide gels. Sepharose 64-71 apolipoprotein B Homo sapiens 14-30 8864858-5 1996 Human HP1 was expressed as a GST-fusion in Escherichia coli and purified with glutathione-Sepharose. Sepharose 90-99 chromobox 5 Homo sapiens 6-9 8984476-1 1996 Acetylcholinesterase (AChE) was purified on columns with iminodiacetate Agarose charged with Cu2+, Zn2+, and Ni2+. Sepharose 72-79 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 8984476-1 1996 Acetylcholinesterase (AChE) was purified on columns with iminodiacetate Agarose charged with Cu2+, Zn2+, and Ni2+. Sepharose 72-79 acetylcholinesterase (Cartwright blood group) Homo sapiens 22-26 8663023-4 1996 Using heparin-Sepharose, we isolated two of the protein forms from immunoaffinity-purified selenoprotein P. Sepharose 14-23 selenoprotein P Rattus norvegicus 91-106 8682309-2 1996 I4L- VV recombinants formed blue plaques when an agarose overlay containing XGluc (5-bromo-4-chloro-3-indolyl-beta-glucuronide) was added to the infected cell monolayer. Sepharose 49-56 ribonucleotide reductase large subunit Vaccinia virus 0-3 8663298-1 1996 Matrix metalloproteinase 3 cleaves insulin-like growth factor-binding protein-3 (IGFBP-3) into six fragments, four of which bind heparin-Sepharose (Fowlkes, J. L., Enghild, J. J., Suzuki, K., and Nagase, H. (1994) J. Biol. Sepharose 137-146 insulin like growth factor binding protein 3 Homo sapiens 35-79 8663298-1 1996 Matrix metalloproteinase 3 cleaves insulin-like growth factor-binding protein-3 (IGFBP-3) into six fragments, four of which bind heparin-Sepharose (Fowlkes, J. L., Enghild, J. J., Suzuki, K., and Nagase, H. (1994) J. Biol. Sepharose 137-146 insulin like growth factor binding protein 3 Homo sapiens 81-88 8670177-10 1996 Furthermore, in all these different respiratory secretions the MUC5AC mucin appears as a similar biochemical entity, as assessed by Mono Q chromatography and agarose electrophoresis, suggesting that it may have a well-defined pattern of glycosylation in the respiratory tract. Sepharose 158-165 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 63-69 8670177-10 1996 Furthermore, in all these different respiratory secretions the MUC5AC mucin appears as a similar biochemical entity, as assessed by Mono Q chromatography and agarose electrophoresis, suggesting that it may have a well-defined pattern of glycosylation in the respiratory tract. Sepharose 158-165 LOC100508689 Homo sapiens 70-75 8672509-6 1996 Preliminary, we screened Lib#1 against human plasmin (PLA, EC 3.4.21.7) immobolized on agarose to enrich for phage displaying variants with PLA affinity. Sepharose 87-94 plasminogen Homo sapiens 45-52 8672509-6 1996 Preliminary, we screened Lib#1 against human plasmin (PLA, EC 3.4.21.7) immobolized on agarose to enrich for phage displaying variants with PLA affinity. Sepharose 87-94 plasminogen Homo sapiens 54-57 8672509-8 1996 Lib#2 (allowing approximately 50 million amino-acid sequences) was screened against PLA-agarose to isolate highest affinity binders. Sepharose 88-95 plasminogen Homo sapiens 84-87 8672510-9 1996 The purified proteins derived from Lib#6 selectants bound to THBN-agarose beads but did not inhibit proteolytic activity of THBN, suggesting that these selectants bind to a site on THBN other than the catalytic site. Sepharose 66-73 coagulation factor II, thrombin Homo sapiens 61-65 8711982-0 1996 Analysis of dystrophin in muscular diseases by two-dimensional gel electrophoresis using agarose gels in the first dimension. Sepharose 89-96 dystrophin Homo sapiens 12-22 8665685-1 1996 Apolipoprotein B gene 3" variable number tandem repeat (VNTR) and related regions were amplified by PCR and analyzed by agarose gel electrophoresis. Sepharose 120-127 apolipoprotein B Homo sapiens 0-16 8681443-4 1996 In this study we show, using agarose gel electrophoresis, that Trp-P-1 inhibits incision by T4 endonuclease V, which cleaves DNA at the site of cyclobutane dimers. Sepharose 29-36 polycystin 1, transient receptor potential channel interacting Homo sapiens 63-70 15566887-7 1996 The products of nested polymerase chain reactions (PCR) amplifying two regions in the IE1 and gp58 genes were detected by agarose gel electrophoresis. Sepharose 122-129 lectin, mannose binding 1 Homo sapiens 94-98 8667637-2 1996 Cordycepin-treated, ADA-inhibited, TdT-positive cells undergo the classic changes associated with drug-induced apoptosis: reduction in cell volume, chromatin clumping, membrane blebbing, and 180-bp multimer DNA laddering on agarose gels. Sepharose 224-231 DNA nucleotidylexotransferase Homo sapiens 35-38 8827452-1 1996 A novel hyaluronan-binding protein (PHBP) was purified from human plasma by affinity chromatography on hyaluronan-conjugated Sepharose. Sepharose 125-134 hyaluronan binding protein 2 Homo sapiens 36-40 8710043-2 1996 This appeared to be due to a macro-enzyme of ASAT, as was demonstrated by protein-A-Sepharose treatment of the serum sample. Sepharose 84-93 ATP binding cassette subfamily B member 7 Homo sapiens 45-49 8843627-4 1996 Two injections of < or = 1 microgram of native PspA purified by use of a choline-Sepharose column are highly immunogenic in BALB/c and CBA/N mice, and even in the absence of adjuvant can elicit protection against otherwise fatal sepsis with 100 times the LD50 of S. pneumoniae. Sepharose 84-93 surfactant associated protein A1 Mus musculus 50-54 8776760-7 1996 The recombinant apoE isoforms were purified, under denaturating conditions, in one step by affinity chromatography on a Ni-chelated agarose column, yielding to about 20 mg of 96% pure protein per liter of culture. Sepharose 132-139 apolipoprotein E Homo sapiens 16-20 8662638-1 1996 Inositol 1,3,4-trisphosphate 5/6-kinase was purified 12,900-fold from calf brain using chromatography on heparin-agarose and affinity elution with inositol hexakisphosphate. Sepharose 113-120 inositol-tetrakisphosphate 1-kinase Bos taurus 0-39 8639781-4 1996 We purified (approximately 10,000-fold) from human plasma a vWF-degrading protease, using chelating Sepharose, hydrophobic interaction chromatography, and gel filtration. Sepharose 100-109 von Willebrand factor Homo sapiens 60-63 8665935-2 1996 A 27-amino-acid synthetic peptide, corresponding to the cytoplasmic domain of rat ICAM-1, was covalently linked to a Sepharose matrix. Sepharose 117-126 intercellular adhesion molecule 1 Rattus norvegicus 82-88 8634257-4 1996 We have characterized the hydrodynamic properties of Triton X-100 solubilized peripherin/rds-rom-1 complexes from bovine ROS membranes by gel exclusion chromatography on Sepharose C1-6B and velocity sedimentation through H2O- and D2O-based sucrose gradients. Sepharose 170-179 peripherin Bos taurus 78-88 8643525-6 1996 Huntingtin from brain extracts is retained on calmodulin(CAM)-Sepharose in a calcium-dependent fashion. Sepharose 62-71 huntingtin Homo sapiens 0-10 8643525-6 1996 Huntingtin from brain extracts is retained on calmodulin(CAM)-Sepharose in a calcium-dependent fashion. Sepharose 62-71 calmodulin 1 Homo sapiens 46-56 8643525-6 1996 Huntingtin from brain extracts is retained on calmodulin(CAM)-Sepharose in a calcium-dependent fashion. Sepharose 62-71 calmodulin 1 Homo sapiens 57-60 8643525-10 1996 Furthermore, an increased amount of mutant huntingtin from HD patient brains is retained on CAM-Sepharose compared to normal huntingtin from control patient brains, and the mutant allele is preferentially retained on CAM-Sepharose in the absence of calcium. Sepharose 96-105 huntingtin Homo sapiens 43-53 8643525-10 1996 Furthermore, an increased amount of mutant huntingtin from HD patient brains is retained on CAM-Sepharose compared to normal huntingtin from control patient brains, and the mutant allele is preferentially retained on CAM-Sepharose in the absence of calcium. Sepharose 96-105 calmodulin 1 Homo sapiens 92-95 8643525-10 1996 Furthermore, an increased amount of mutant huntingtin from HD patient brains is retained on CAM-Sepharose compared to normal huntingtin from control patient brains, and the mutant allele is preferentially retained on CAM-Sepharose in the absence of calcium. Sepharose 221-230 huntingtin Homo sapiens 43-53 8643525-10 1996 Furthermore, an increased amount of mutant huntingtin from HD patient brains is retained on CAM-Sepharose compared to normal huntingtin from control patient brains, and the mutant allele is preferentially retained on CAM-Sepharose in the absence of calcium. Sepharose 221-230 calmodulin 1 Homo sapiens 217-220 8796479-3 1996 The eluate was diluted 2-fold, then subjected to an immunoaffinity column, anti-CR1 (named 31R)-conjugated to Sepharose. Sepharose 110-119 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 80-83 8629620-5 1996 We isolated total IgA, in plasma by jacalin-agarose. Sepharose 44-51 CD79a molecule Homo sapiens 18-21 8645144-4 1996 The macromolecules were fragmented by reduction into two distinct populations of MUC2 subunits as assessed by agarose electrophoresis. Sepharose 110-117 mucin 2, oligomeric mucus/gel-forming Homo sapiens 81-85 8963723-8 1996 When applied to plasma samples, the LBS activity of Lp(a) ranged from 0% to 100% of an isolated reference Lp(a); the signal corresponded to the percent retention of Lp(a) on a lysine-Sepharose but did not correlate well with total Lp(a) levels in plasma. Sepharose 183-192 lipoprotein(a) Homo sapiens 52-57 8963724-2 1996 Cell extracts were then prepared and subjected to affinity chromatography on diisopropyl fluorophosphate (DFP)-inactivated TPA-Sepharose 4B. Sepharose 127-139 plasminogen activator, tissue type Homo sapiens 123-126 8963724-3 1996 Approximately 6% of the incorporated 35S radioactivity bound to DFP-treated TPA-Sepharose 4B and was eluted with 2 mol/L NaCl. Sepharose 80-89 plasminogen activator, tissue type Homo sapiens 76-79 8647124-12 1996 Using the biotinylated cholecystokinin derivative the photoaffinity-labeled CCKB receptor could be purified 1260-fold by a two-step procedure including affinity chromatography on a streptavidin/avidin agarose matrix. Sepharose 201-208 cholecystokinin B receptor Homo sapiens 76-80 8636239-4 1996 We have found that eleven proteins from embryonic chick brain membranes consistently bind to and elute from a DM-GRASP-Sepharose affinity column. Sepharose 119-128 activated leukocyte cell adhesion molecule Gallus gallus 110-118 8636239-8 1996 The DM-GRASP-Sepharose eluate also contains a potent neurite stimulating activity, which cannot be accounted for by either DM-GRASP or NgCAM. Sepharose 13-22 activated leukocyte cell adhesion molecule Gallus gallus 4-12 8636239-8 1996 The DM-GRASP-Sepharose eluate also contains a potent neurite stimulating activity, which cannot be accounted for by either DM-GRASP or NgCAM. Sepharose 13-22 activated leukocyte cell adhesion molecule Gallus gallus 123-131 8633059-2 1996 The PLD enzyme associated with particulate fractions was solubilized by deoxycholate and partially purified by chromatography on a heparin-Sepharose column. Sepharose 139-148 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 4-7 8626599-6 1996 Like mammalian GBPs, recombinant chicken GBP specifically bound to agarose-immobilized guanine nucleotides and hydrolyzed GTP to both GDP and GMP. Sepharose 67-74 guanylate binding protein Gallus gallus 15-18 8621623-8 1996 GST-TEF-1 fusion peptides fixed to glutathione-Sepharose beads retained in vitro-generated human TATA-binding protein, hTBP. Sepharose 47-56 TEA domain transcription factor 1 Homo sapiens 4-9 8621623-8 1996 GST-TEF-1 fusion peptides fixed to glutathione-Sepharose beads retained in vitro-generated human TATA-binding protein, hTBP. Sepharose 47-56 TATA-box binding protein Homo sapiens 97-117 8621623-8 1996 GST-TEF-1 fusion peptides fixed to glutathione-Sepharose beads retained in vitro-generated human TATA-binding protein, hTBP. Sepharose 47-56 TATA-box binding protein Homo sapiens 119-123 8738019-3 1996 Additional protein G Sepharose treatment leads to an IgA preparation of greater than 99% purity. Sepharose 21-30 CD79a molecule Homo sapiens 53-56 8605204-2 1996 A polyhistidine extension was incorporated at the C-terminus of the expressed protein, which, after purification of the protein on a nickel-agarose column, could be removed proteolytically by treatment with thrombin. Sepharose 140-147 coagulation factor II, thrombin Homo sapiens 207-215 8639272-1 1996 The transcriptional regulator of the Citrobacter freundii ampC beta-lactamase gene, AmpR, was purified as a single SDS/PAGE-gel band by using various techniques, including DNA-Sepharose 4B affinity chromatography. Sepharose 176-185 lysR-type transcriptional regulator AmpR Citrobacter freundii 84-88 8630104-6 1996 FN was purified on gelatin-agarose columns and further characterized by polyacrylamide gel electrophoresis. Sepharose 27-34 fibronectin 1 Bos taurus 0-2 8670096-1 1996 Purified human serum butyrylcholinesterase after treatment with either of the metal chelators EDTA or NaCN was able to bind to a Zn(2+)-chelate-Sepharose affinity column and was eluted from the column by EDTA or imidazole. Sepharose 144-153 butyrylcholinesterase Homo sapiens 21-42 8818262-2 1996 It makes use of batch adsorption on Procion Red H-3B coupled to agarose through an amine linkage. Sepharose 64-71 H3 clustered histone 4 Homo sapiens 48-52 8743563-4 1996 Binding of 125I-anti-band 3 IgG to band 3-Sepharose gel was partially inhibited by band 3 oligosaccharides or lactoferrin, but was less inhibited by them after they had been treated with N-glycosidase F or endo-beta-galactosidase. Sepharose 42-51 galactosidase beta 1 Homo sapiens 211-229 8925451-1 1996 Cathepsin D was purified from ovaries of Xenopus laevis by both QAE-cellulose and pepstatin-Sepharose chromatography and then characterized and compared with Xenopus liver cathepsin D. Sepharose 92-101 cathepsin D Xenopus laevis 0-11 8741012-2 1996 Mucin secretion was followed by measuring the release of [3H]-glucosamine metabolically labelled glycoproteins eluted in the void volume of Sepharose 4B column chromatography. Sepharose 140-149 LOC100508689 Homo sapiens 0-5 8743558-2 1996 Fibronectin receptor in several cell lysates was bound to a column of C279-immobilized Sepharose HP and obtained in a highly purified form by elution with a synthetic peptide, GRGDSP. Sepharose 87-96 fibronectin 1 Homo sapiens 0-11 8992886-3 1996 When cultured in agarose gel containing 100 ng/ml of recombinant human bone morphogenetic protein 2 (rhBMP-2; BMP-2), RMD-1 cells formed colonies and showed chondrocyte-like features as assessed by their ultrastructure, metachromatic staining with toluidine blue, and the production of large hydrodynamic-size proteoglycans. Sepharose 17-24 bone morphogenetic protein 2 Homo sapiens 71-99 8992886-3 1996 When cultured in agarose gel containing 100 ng/ml of recombinant human bone morphogenetic protein 2 (rhBMP-2; BMP-2), RMD-1 cells formed colonies and showed chondrocyte-like features as assessed by their ultrastructure, metachromatic staining with toluidine blue, and the production of large hydrodynamic-size proteoglycans. Sepharose 17-24 bone morphogenetic protein 2 Homo sapiens 103-108 8992886-3 1996 When cultured in agarose gel containing 100 ng/ml of recombinant human bone morphogenetic protein 2 (rhBMP-2; BMP-2), RMD-1 cells formed colonies and showed chondrocyte-like features as assessed by their ultrastructure, metachromatic staining with toluidine blue, and the production of large hydrodynamic-size proteoglycans. Sepharose 17-24 RMD1 Homo sapiens 118-123 8786322-7 1996 These bands were also recovered with CD21 precipitated from MT2 cells with C3d bound to Sepharose via the internal thioester, but were absent in CD21-expressing B cell lines. Sepharose 88-97 complement C3d receptor 2 Homo sapiens 37-41 8786322-7 1996 These bands were also recovered with CD21 precipitated from MT2 cells with C3d bound to Sepharose via the internal thioester, but were absent in CD21-expressing B cell lines. Sepharose 88-97 metallothionein 2A Homo sapiens 60-63 8786322-7 1996 These bands were also recovered with CD21 precipitated from MT2 cells with C3d bound to Sepharose via the internal thioester, but were absent in CD21-expressing B cell lines. Sepharose 88-97 endogenous retrovirus group K member 13 Homo sapiens 75-78 8613703-3 1996 Affinity chromatography with serglycin-Sepharose and chondroitin sulphate-Sepharose was used to isolate proteoglycin-binding proteins from macrophages and platelets. Sepharose 39-48 serglycin Homo sapiens 29-38 8613703-9 1996 Amino-terminal sequencing of two proteins from platelet extracts that bound to serglycin-Sepharose revealed that they corresponded to multimeric forms of human platelet factor 4 (PE4). Sepharose 89-98 serglycin Homo sapiens 79-88 8613703-9 1996 Amino-terminal sequencing of two proteins from platelet extracts that bound to serglycin-Sepharose revealed that they corresponded to multimeric forms of human platelet factor 4 (PE4). Sepharose 89-98 platelet factor 4 Homo sapiens 160-177 8613703-9 1996 Amino-terminal sequencing of two proteins from platelet extracts that bound to serglycin-Sepharose revealed that they corresponded to multimeric forms of human platelet factor 4 (PE4). Sepharose 89-98 platelet factor 4 Homo sapiens 179-182 8613703-10 1996 Chondroitin sulfate-Sepharose was shown to be equally efficient in retaining PF4 from platelet extracts as serglycin-Sepharose indicating that the glycosaminoglycan chains mediate the binding to PF4 in the intact proteoglycan molecule. Sepharose 20-29 platelet factor 4 Homo sapiens 77-80 8613703-10 1996 Chondroitin sulfate-Sepharose was shown to be equally efficient in retaining PF4 from platelet extracts as serglycin-Sepharose indicating that the glycosaminoglycan chains mediate the binding to PF4 in the intact proteoglycan molecule. Sepharose 20-29 platelet factor 4 Homo sapiens 195-198 8613703-10 1996 Chondroitin sulfate-Sepharose was shown to be equally efficient in retaining PF4 from platelet extracts as serglycin-Sepharose indicating that the glycosaminoglycan chains mediate the binding to PF4 in the intact proteoglycan molecule. Sepharose 117-126 serglycin Homo sapiens 107-116 8740442-5 1996 CSD was immunotrapped from brain and liver tissue supernatant using a specific CSD antiserum and Protein-A Sepharose. Sepharose 107-116 cysteine sulfinic acid decarboxylase Homo sapiens 0-3 9148599-1 1996 Human blood plasma fibronectin immobilised on agarose in physiological interacts with soluble myeloperoxidase (Kd = 2.43 mM). Sepharose 46-53 fibronectin 1 Homo sapiens 19-30 9148599-1 1996 Human blood plasma fibronectin immobilised on agarose in physiological interacts with soluble myeloperoxidase (Kd = 2.43 mM). Sepharose 46-53 myeloperoxidase Homo sapiens 94-109 8612795-1 1996 Hemopexin, the heme-binding serum glycoprotein, exhibits a complex electrophoretic pattern on two-dimensional immunoelectrophoresis on agarose gels into which hyaluronic acid is incorporated in the first and monospecific anti-hemopexin in the second dimension. Sepharose 135-142 hemopexin Homo sapiens 0-9 8636075-2 1996 This fusion protein, called DysS9, bound to calmodulin-Sepharose, bound biotinylated calmodulin, caused characteristic changes in the fluorescence emission spectrum of dansyl-calmodulin, and had an apparent affinity for dansyl-calmodulin of 54 nM. Sepharose 55-64 calmodulin 2 Mus musculus 44-54 8615756-6 1996 Serum increased PAI-1 expression in NRK cells in agarose suspension, as well as monolayer, culture; induction in suspended cells (similar to monolayer culture conditions) also occurred in the presence of cyclohexamide or puromycin. Sepharose 49-56 serpin family E member 2 Rattus norvegicus 16-21 8615786-14 1996 The nucleosomal necdin complex (>200 kDa) was adsorbed on an organomercurial agarose affinity chromatography column and was eluted with 10 mM DTT, revealing that necdin is possibly involved in the transactive nucleosomal complex. Sepharose 80-87 necdin, MAGE family member Mus musculus 16-22 8615786-14 1996 The nucleosomal necdin complex (>200 kDa) was adsorbed on an organomercurial agarose affinity chromatography column and was eluted with 10 mM DTT, revealing that necdin is possibly involved in the transactive nucleosomal complex. Sepharose 80-87 necdin, MAGE family member Mus musculus 165-171 8621443-11 1996 The GST-VEGF-exon 7 fusion protein bound to heparin-Sepharose with a similar affinity as VEGF165 and inhibited the binding of 125I-VEGF165 to 231 cells. Sepharose 52-61 glutathione S-transferase kappa 1 Homo sapiens 4-7 8621443-11 1996 The GST-VEGF-exon 7 fusion protein bound to heparin-Sepharose with a similar affinity as VEGF165 and inhibited the binding of 125I-VEGF165 to 231 cells. Sepharose 52-61 vascular endothelial growth factor A Homo sapiens 8-12 8721305-11 1996 The well-known adsorbent Cibacron blue 3GA-agarose exhibited 8% lower recovery and 25% lower purification for mMDH. Sepharose 43-50 malate dehydrogenase 2, NAD (mitochondrial) Mus musculus 110-114 8614006-6 1996 c-myc mRNA expression was determined by northern blot analysis, protein expression by western blot analysis, cell growth inhibition counts, and DNA cleavage by agarose gel electrophoretic analysis. Sepharose 160-167 MYC proto-oncogene, bHLH transcription factor Homo sapiens 0-5 8687140-3 1996 The competitor RNA harbors a 19-base deletion and 63-base insertion compared to wild-type c-erbB-2 mRNA and generates a PCR product which is easily distinguished from the wild-type PCR product by agarose gel electrophoresis. Sepharose 196-203 erb-b2 receptor tyrosine kinase 2 Homo sapiens 90-98 8670079-7 1996 HD2 dissociated into HD1 when treated with 1.6 M NaCl or when applied to a Q-Sepharose column. Sepharose 77-86 histone deacetylase 2 Gallus gallus 0-3 8670080-1 1996 A soluble 67 kDa angiotensin-converting enzyme (ACE) has been purified by lisinopril-Sepharose affinity column chromatography from adult houseflies, Musca domestica. Sepharose 85-94 angiotensin-converting enzyme Musca domestica 17-46 8670080-1 1996 A soluble 67 kDa angiotensin-converting enzyme (ACE) has been purified by lisinopril-Sepharose affinity column chromatography from adult houseflies, Musca domestica. Sepharose 85-94 angiotensin-converting enzyme Musca domestica 48-51 8634427-3 1996 Purified or plasma vWF was immobilized with a monoclonal antibody (MoAb RU1) covalently linked to Sepharose (Pharmacia LKB Biotechnology, Uppsala, Sweden). Sepharose 98-107 von Willebrand factor Homo sapiens 19-22 8634427-4 1996 Factor VIII was incubated with vWF-RU1-Sepharose and unbound factor VIII was separated from bound factor VIII by centrifugation. Sepharose 39-48 von Willebrand factor Homo sapiens 31-34 8634427-4 1996 Factor VIII was incubated with vWF-RU1-Sepharose and unbound factor VIII was separated from bound factor VIII by centrifugation. Sepharose 39-48 Scm like with four mbt domains 1 Homo sapiens 35-38 8634427-6 1996 Factor VIII binding to vWF-RU1-Sepharose conformed to the Langmuir model for independent binding sites with a Kd of 0.46 +/- 0.12 nmol/L, and a stoichiometry of 1.3 factor VIII molecules per vWF monomer at saturation, suggesting that each vWF subunit contains a binding site for factor VIII. Sepharose 31-40 von Willebrand factor Homo sapiens 23-26 8634427-6 1996 Factor VIII binding to vWF-RU1-Sepharose conformed to the Langmuir model for independent binding sites with a Kd of 0.46 +/- 0.12 nmol/L, and a stoichiometry of 1.3 factor VIII molecules per vWF monomer at saturation, suggesting that each vWF subunit contains a binding site for factor VIII. Sepharose 31-40 Scm like with four mbt domains 1 Homo sapiens 27-30 8634427-6 1996 Factor VIII binding to vWF-RU1-Sepharose conformed to the Langmuir model for independent binding sites with a Kd of 0.46 +/- 0.12 nmol/L, and a stoichiometry of 1.3 factor VIII molecules per vWF monomer at saturation, suggesting that each vWF subunit contains a binding site for factor VIII. Sepharose 31-40 von Willebrand factor Homo sapiens 191-194 8634427-6 1996 Factor VIII binding to vWF-RU1-Sepharose conformed to the Langmuir model for independent binding sites with a Kd of 0.46 +/- 0.12 nmol/L, and a stoichiometry of 1.3 factor VIII molecules per vWF monomer at saturation, suggesting that each vWF subunit contains a binding site for factor VIII. Sepharose 31-40 von Willebrand factor Homo sapiens 191-194 8634427-8 1996 DeltaA2-rvWF effectively displaced previously bound factor VIII, confirming that factor VIII binding to vWF-RU1-Sepharose was reversible. Sepharose 112-121 von Willebrand factor Homo sapiens 9-12 8634427-8 1996 DeltaA2-rvWF effectively displaced previously bound factor VIII, confirming that factor VIII binding to vWF-RU1-Sepharose was reversible. Sepharose 112-121 Scm like with four mbt domains 1 Homo sapiens 108-111 8634427-9 1996 To determine the association rate constant (k(on)) and the dissociation rate constant (k(off)), factor VIII was incubated with vWF-RU1-Sepharose for various time intervals. Sepharose 135-144 von Willebrand factor Homo sapiens 127-130 8634427-9 1996 To determine the association rate constant (k(on)) and the dissociation rate constant (k(off)), factor VIII was incubated with vWF-RU1-Sepharose for various time intervals. Sepharose 135-144 Scm like with four mbt domains 1 Homo sapiens 131-134 8634427-11 1996 Similar values were obtained from the dissociation kinetics measured after dilution of preformed factor VIII-vWF-RU1-Sepharose complexes. Sepharose 117-126 von Willebrand factor Homo sapiens 109-112 8634427-11 1996 Similar values were obtained from the dissociation kinetics measured after dilution of preformed factor VIII-vWF-RU1-Sepharose complexes. Sepharose 117-126 Scm like with four mbt domains 1 Homo sapiens 113-116 8598109-0 1996 Relation between number of apolipoprotein(a) kringle 4 repeats and mobility of isoforms in agarose gel: basis for a standardized isoform nomenclature. Sepharose 91-98 lipoprotein(a) Homo sapiens 27-44 8598109-2 1996 Plasma apo(a) isoform sizes were determined by a high-resolution sodium dodecyl sulfate-agarose gel electrophoretic method, followed by immunoblotting. Sepharose 88-95 lipoprotein(a) Homo sapiens 7-13 8598109-3 1996 The relation between the number of apo(a) kringle 4-encoding sequences in the apo(a) gene, as assessed by pulsed-field gel electrophoresis and genomic blotting, and the mobility of apo(a) isoforms in agarose gel was evaluated in 29 individuals who had 48 expressed apo(a) isoforms that contained between 12 and 41 kringle 4 repeats. Sepharose 200-207 lipoprotein(a) Homo sapiens 78-84 8598109-3 1996 The relation between the number of apo(a) kringle 4-encoding sequences in the apo(a) gene, as assessed by pulsed-field gel electrophoresis and genomic blotting, and the mobility of apo(a) isoforms in agarose gel was evaluated in 29 individuals who had 48 expressed apo(a) isoforms that contained between 12 and 41 kringle 4 repeats. Sepharose 200-207 lipoprotein(a) Homo sapiens 78-84 8598109-3 1996 The relation between the number of apo(a) kringle 4-encoding sequences in the apo(a) gene, as assessed by pulsed-field gel electrophoresis and genomic blotting, and the mobility of apo(a) isoforms in agarose gel was evaluated in 29 individuals who had 48 expressed apo(a) isoforms that contained between 12 and 41 kringle 4 repeats. Sepharose 200-207 lipoprotein(a) Homo sapiens 78-84 8665801-1 1996 Retinol-binding protein (RBP) was purified from Day 60 porcine allantoic fluid by a combination of diethylaminoethyl cellulose, G-100 Sephadex, G-50 Sephadex, Phenyl-Sepharose, and Reactive Green 19-dye-agarose chromatography. Sepharose 203-210 retinol binding protein 4 Homo sapiens 25-28 8743563-5 1996 A significant part of 125I-anti-band 3 IgG that bound to the band 3-Sepharose gel was released upon treatment of the gel with N-glycosidase F or endo-beta-galactosidase. Sepharose 68-77 galactosidase beta 1 Homo sapiens 150-168 8773545-2 1996 They were subsequently chromatographed on a lipoprotein lipase (LpL)-Sepharose column. Sepharose 69-78 lipoprotein lipase Homo sapiens 64-67 8617783-3 1996 The combined effect of two histidine mutants, E30H and Q62H, gave thioredoxin the capacity to bind to nickel ions immobilized on iminodiacetic acid- and nitrilotriacetic acid-Sepharose resins. Sepharose 175-184 thioredoxin Homo sapiens 66-77 8596020-4 1996 Affinity-purified Ag-specific GIF preparations, obtained by using Ag-coupled Sepharose or anti-TCR alpha-chain-coupled Affi-Gel, contained a 55-kDa peptide, which bound both polyclonal anti-GIF Abs and anti-TCR-alpha mAb in immunoblotting. Sepharose 77-86 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 30-33 8830055-4 1996 Very-long-chain acyl-CoA synthetase has been extracted from the washed membrane fraction of frozen rat liver peroxisomes with a buffer containing a detergent, and has been purified by chromatography on Ultrogel AcA 34, calcium phosphate gel/cellulose, blue dextran-Sepharose 4B and DEAE-Toyopearl. Sepharose 265-274 solute carrier family 27 member 2 Rattus norvegicus 0-35 8648928-4 1996 Total IgA1 in plasma from patients or healthy controls was isolated by jacalin-agarose column as jacalin-bound proteins (JBP). Sepharose 79-86 immunoglobulin heavy constant alpha 1 Homo sapiens 6-10 8849037-3 1996 Selenoprotein-P is retained on a heparin-Sepharose column, and subsequently eluted with an excess of heparin, while glutathione peroxidase is separated by a blue-Sepharose column. Sepharose 41-50 selenoprotein P Homo sapiens 0-15 8621687-4 1996 Deletion mutants of CyP-40 fused to glutathione S-transferase were immobilized on glutathione-agarose and then used in a rapid hsp90 retention assay to define regions of the CyP-40 C terminus that are important for hsp90 binding. Sepharose 94-101 peptidylprolyl isomerase D Homo sapiens 20-26 8619803-2 1996 Recombinant (r) PrP27-30 corresponding to aa 90-231 from the Syrian golden hamster prion protein was expressed as a fusion with GST in E. coli and secreted from insect cells infected with recombinant baculoviruses, GST::rPrP27-30 isolated from either system was purified to homogenity by glutathione-Sepharose chromatography. Sepharose 300-309 major prion protein Mesocricetus auratus 16-24 8812855-2 1996 Human placental RNase inhibitor was conjugated to CNBr-activated Sepharose in the presence of dithiothreitol. Sepharose 65-74 ribonuclease/angiogenin inhibitor 1 Homo sapiens 6-31 8812719-5 1996 The amplified cDNA message for the growth factors and beta-actin migrated as a discrete band at the expected base pair number on agarose gels stained with the intercalating fluorescent dye ethidium bromide. Sepharose 129-136 actin, beta Mus musculus 54-64 8611172-6 1996 When tissue extracts were applied to columns of heparin-agarose and eluted by a gradient of NaCl, a peak of active LPL was eluted at 1.0 M NaCl, but there was also a peak of inactive LPL protein, which was eluted at 0.6 M NaCl. Sepharose 56-63 lipoprotein lipase Rattus norvegicus 115-118 8611172-8 1996 The mass ratio between inactive and active LPL, as separated by heparin-agarose chromatography, increased from 0.5 to over 2 during the fast. Sepharose 72-79 lipoprotein lipase Rattus norvegicus 43-46 9064288-6 1996 Both affinity purified (C4b Sepharose) and chromatographically isolated (using jacqualine agarose) sgp 120 were recognized by B1.9.E-2. Sepharose 28-37 complement component 4B (Chido blood group) Mus musculus 24-27 8598223-8 1996 With the use of Ugi-Sepharose affinity chromatography, we show that a second and distinct uracil-DNA glycosylase is associated with the nuclear compartment. Sepharose 20-29 uracil DNA glycosylase Homo sapiens 90-112 8907612-1 1996 We recently demonstrated that keratinocyte growth factor (KGF), a fibroblast-derived growth factor, induced anchorage-independent growth of HPV16 DNA-immortalized human uterine exocervical epithelial cells (HCE16/3 cell line) in soft agarose assay. Sepharose 234-241 fibroblast growth factor 7 Homo sapiens 58-61 8645623-1 1996 The kinetics of the interaction between recombinant human estrogen receptor and chicken vitellogenin gene II estrogen response element (ERE) were determined by ERE-Sepharose chromatography. Sepharose 164-173 estrogen receptor 1 Homo sapiens 58-75 8882708-1 1996 Recombinant human eukaryotic initiation factor-4E (eIF-4E), purified by m7GTP-Sepharose 4B affinity chromatography, was used for crystallization. Sepharose 78-87 eukaryotic translation initiation factor 4E Homo sapiens 18-49 8882708-1 1996 Recombinant human eukaryotic initiation factor-4E (eIF-4E), purified by m7GTP-Sepharose 4B affinity chromatography, was used for crystallization. Sepharose 78-87 eukaryotic translation initiation factor 4E Homo sapiens 51-57 8882644-1 1996 A reverse transcription (RT) nested polymerase chain reaction (PCR) procedure is described for detecting RNA to a spliced late gene (SLG) of human cytomegalovirus (CMV), the product of which (175 bp) is easily differentiated in agarose gels from the product when the target is unspliced viral RNA or DNA (258 bp). Sepharose 228-235 sialic acid binding Ig like lectin 12 Homo sapiens 133-136 8821828-6 1996 The effect of heparin on c-fos induction may be independent of interaction with cytokines or cytokine receptors; its magnitude is not diminished when heparin-binding substances are removed from serum by heparin-Sepharose. Sepharose 211-220 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 25-30 8920005-2 1996 The DHFR domain was purified to homogeneity by methotrexate-Sepharose chromatography followed by an anion-exchange chromatography step in a mono Q column, and displayed a single 27-kDa band on SDS-PAGE. Sepharose 60-69 Dihydrofolate reductase Escherichia coli 4-8 8744421-1 1996 Mevalonate pyrophosphate decarboxylase was isolated from rat liver to 90% purity as judged by SDS-PAGE using Phenyl Sepharose, p-coumaric acid-Sepharose, Mono P, and Mono Q chromatography. Sepharose 116-125 mevalonate diphosphate decarboxylase Rattus norvegicus 0-38 8785405-5 1996 Silver-stained two-dimensional gels of renin separated from kidney homogenate with pepstatin agarose confirm the presence of an acidic renin isoform in CsA-treated rats. Sepharose 93-100 renin Rattus norvegicus 39-44 8785405-5 1996 Silver-stained two-dimensional gels of renin separated from kidney homogenate with pepstatin agarose confirm the presence of an acidic renin isoform in CsA-treated rats. Sepharose 93-100 renin Rattus norvegicus 135-140 8820970-6 1996 The amphiphilic properties of the several AChE and BuChE molecules were analyzed by Triton X-114 phase-partitioning and by phenyl-agarose chromatography. Sepharose 130-137 acetylcholinesterase Mus musculus 42-46 8579612-3 1996 The fusion protein adsorbed to glutathione sepharose was cleaved with thrombin to yield soluble 105-427 human 1,25-dihydroxyvitamin D3 receptor. Sepharose 43-52 vitamin D receptor Homo sapiens 110-143 8567691-4 1996 Purification of GCS was achieved via a two-step dye-agarose chromatography procedure using UDP-Glc to elute the enzyme. Sepharose 52-59 UDP-glucose ceramide glucosyltransferase Rattus norvegicus 16-19 8576121-4 1996 We found that detergent-solubilized pIgR binds to CaM-agarose in a Ca(2+)-dependent fashion, and binding is inhibited by the addition of excess free CaM or the CaM antagonist W-13 (N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide), suggesting that pIgR binding to CaM is specific. Sepharose 54-61 polymeric immunoglobulin receptor Canis lupus familiaris 36-40 8576121-4 1996 We found that detergent-solubilized pIgR binds to CaM-agarose in a Ca(2+)-dependent fashion, and binding is inhibited by the addition of excess free CaM or the CaM antagonist W-13 (N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide), suggesting that pIgR binding to CaM is specific. Sepharose 54-61 calmodulin 1 Rattus norvegicus 50-53 8576121-4 1996 We found that detergent-solubilized pIgR binds to CaM-agarose in a Ca(2+)-dependent fashion, and binding is inhibited by the addition of excess free CaM or the CaM antagonist W-13 (N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide), suggesting that pIgR binding to CaM is specific. Sepharose 54-61 calmodulin 1 Rattus norvegicus 149-152 8576121-4 1996 We found that detergent-solubilized pIgR binds to CaM-agarose in a Ca(2+)-dependent fashion, and binding is inhibited by the addition of excess free CaM or the CaM antagonist W-13 (N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide), suggesting that pIgR binding to CaM is specific. Sepharose 54-61 calmodulin 1 Rattus norvegicus 149-152 8576121-4 1996 We found that detergent-solubilized pIgR binds to CaM-agarose in a Ca(2+)-dependent fashion, and binding is inhibited by the addition of excess free CaM or the CaM antagonist W-13 (N-(4-aminobutyl)-5-chloro-2-naphthalenesulfonamide), suggesting that pIgR binding to CaM is specific. Sepharose 54-61 calmodulin 1 Rattus norvegicus 149-152 9172774-10 1996 Further, the radioligand equilibrium binding constant for this interaction has been determined by immobilizing EBP on agarose gel via a free cysteine. Sepharose 118-125 EBP cholestenol delta-isomerase Homo sapiens 111-114 8547345-3 1996 The apo-form of the folate binding protein thus obtained was purified by affinity chromatography using pteroylglutamic acid covalently coupled to Sepharose 4B. Sepharose 146-158 glycine N-methyltransferase Rattus norvegicus 20-42 8557634-6 1996 Affinity chromatography of human plasma on Sepharose coupled with the protein specifically absorbed two plasma proteins which were identified as clusterin and histidine-rich glycoprotein (HRG). Sepharose 43-52 histidine rich glycoprotein Homo sapiens 159-186 8557634-6 1996 Affinity chromatography of human plasma on Sepharose coupled with the protein specifically absorbed two plasma proteins which were identified as clusterin and histidine-rich glycoprotein (HRG). Sepharose 43-52 histidine rich glycoprotein Homo sapiens 188-191 8788251-2 1996 The rabbit anti-NKR antibody was raised against a bacterial fusion protein containing a C-terminal portion of NKR and affinity purified with a Sepharose 4B column conjugated to the fusion protein. Sepharose 143-152 tachykinin receptor 3 Rattus norvegicus 16-19 8789157-2 1996 The method allows direct detection of small amounts of diamine oxidase in serum samples after agarose gel electrophoresis and allows visualization of diamine oxidase activity in tissue sections. Sepharose 94-101 amine oxidase copper containing 1 Homo sapiens 55-70 8825618-3 1996 CD4-free tryptic glycopeptides, prepared from the membrane of CD4+ monocytic U937 cells and partially purified by ConA-agarose affinity chromatography, could be eluted by mannan but not by methyl-alpha-mannose or methyl-alpha-glucose, which strongly suggests that they displayed oligomannosidic structures. Sepharose 119-126 CD4 molecule Homo sapiens 0-3 11178476-0 1996 Production and Purification of Mouse IgG Subclass Specific Chicken Egg Yolk Antibodies Using a New Indirect Affinity Chromatography Method with Protein G Sepharose. Sepharose 154-163 immunoglobulin heavy variable V1-62 Mus musculus 37-40 8574094-7 1996 The interaction of ceruloplasmin with the chromatographic material used for its isolation, Sepharose 4B derivatized with chloroethylamine, was weakened by calcium. Sepharose 91-103 ceruloplasmin Homo sapiens 19-32 8546710-4 1996 We have purified native and recombinant human DBH by a modified purification procedure using SP-Sepharose, lentil lectin-Sepharose and gel-filtration chromatography and carried out studies to compare the two enzymes. Sepharose 96-105 dopamine beta-hydroxylase Homo sapiens 46-49 8913639-10 1996 Two monoclonal antibodies were cloned that react with p73 present in fractions released from beta actin Sepharose-4B or purified to homogeneity by DEAE chromatography. Sepharose 104-116 transformation related protein 73 Mus musculus 54-57 8913639-13 1996 Anti-p73 Western blotting reveals that nanomolar concentrations of CD are capable of selectively eluting p73 and ezrin from beta actin Sepharose 4B, indicating that p73 binds beta actin via the barbed end. Sepharose 135-144 transformation related protein 73 Mus musculus 105-108 8913639-13 1996 Anti-p73 Western blotting reveals that nanomolar concentrations of CD are capable of selectively eluting p73 and ezrin from beta actin Sepharose 4B, indicating that p73 binds beta actin via the barbed end. Sepharose 135-144 transformation related protein 73 Mus musculus 105-108 9084679-1 1996 Bone sialoprotein (BSP) was shown to be a potent nucleator of hydroxyapatite (HA) in a steady-state agarose gel system (Hunter and Goldberg, 1993, PNAS 90: 8562). Sepharose 100-107 integrin binding sialoprotein Homo sapiens 0-23 9084679-7 1996 In the steady-state agarose gel system, BSP, P1 and P2 induced HA formation, whereas the pooled small BSP-derived peptides (P3-5) did not. Sepharose 20-27 integrin binding sialoprotein Homo sapiens 40-43 8742062-1 1996 Recombinant chicken stem cell factor (SCF) was produced in bacteria as a histidine-tagged protein (His delta SCF) and purified by affinity chromatography on an Ni(2+)-NTA agarose column. Sepharose 171-178 KIT ligand Gallus gallus 20-36 8742062-1 1996 Recombinant chicken stem cell factor (SCF) was produced in bacteria as a histidine-tagged protein (His delta SCF) and purified by affinity chromatography on an Ni(2+)-NTA agarose column. Sepharose 171-178 KIT ligand Gallus gallus 38-41 8565823-7 1996 Epidermal growth factor (EGF) and fibroblast growth factor-4 (FGF-4) inhibited apoptosis in the dental mesenchyme when applied locally using agarose or heparin-coated acrylic beads, suggesting involvement of these or related growth factors in the prevention of apoptosis in dental tissues in vivo. Sepharose 141-148 epidermal growth factor Mus musculus 0-23 8565823-7 1996 Epidermal growth factor (EGF) and fibroblast growth factor-4 (FGF-4) inhibited apoptosis in the dental mesenchyme when applied locally using agarose or heparin-coated acrylic beads, suggesting involvement of these or related growth factors in the prevention of apoptosis in dental tissues in vivo. Sepharose 141-148 epidermal growth factor Mus musculus 25-28 8565823-7 1996 Epidermal growth factor (EGF) and fibroblast growth factor-4 (FGF-4) inhibited apoptosis in the dental mesenchyme when applied locally using agarose or heparin-coated acrylic beads, suggesting involvement of these or related growth factors in the prevention of apoptosis in dental tissues in vivo. Sepharose 141-148 fibroblast growth factor 4 Mus musculus 34-60 8565823-7 1996 Epidermal growth factor (EGF) and fibroblast growth factor-4 (FGF-4) inhibited apoptosis in the dental mesenchyme when applied locally using agarose or heparin-coated acrylic beads, suggesting involvement of these or related growth factors in the prevention of apoptosis in dental tissues in vivo. Sepharose 141-148 fibroblast growth factor 4 Mus musculus 62-67 8907516-2 1996 The performance of agarose and polyacrylamide (PAGE) gels in quantitating polymerase chain reaction (PCR) amplified c-erbB2 and p53 gene sequences (213 and 133 base pairs, respectively) was studied by applying image analysis on photographed ethidium bromide stained gels. Sepharose 19-26 erb-b2 receptor tyrosine kinase 2 Homo sapiens 116-123 8681140-0 1996 Long RT-PCR of the entire 8.5-kb NF1 open reading frame and mutation detection on agarose gels. Sepharose 82-89 neurofibromin 1 Homo sapiens 33-36 8557340-6 1996 65-kDa fibronectin-binding protein of M. penetrans was eluted following Sepharose-fibronectin affinity chromatography. Sepharose 72-81 fibronectin 1 Homo sapiens 7-18 8557340-6 1996 65-kDa fibronectin-binding protein of M. penetrans was eluted following Sepharose-fibronectin affinity chromatography. Sepharose 72-81 fibronectin 1 Homo sapiens 82-93 8639481-4 1996 Alb-hep surfaces incubated in plasma which was preexposed to heparin-Sepharose retained 30% of their initial activity. Sepharose 69-78 albumin Homo sapiens 0-3 8649192-2 1996 We show here that enzymatically active recombinant CLC binds to a lactose-conjugated agarose resin, and that binding is inhibited in a dose dependent fashion by both lactose (IC50 = 41 mM) and fucose (IC50 = 380 mM), but not by arabinose. Sepharose 85-92 Charcot-Leyden crystal galectin Homo sapiens 51-54 9112222-5 1996 The p25 antigen was expressed as a glutathione S-transferase-fusion protein in E. coli and purified with glutathione-sepharose. Sepharose 117-126 P-25 Drosophila melanogaster 4-7 8576617-6 1996 Thirdly the pooled fractions which contained PAPP-A after Heparin-Sepharose affinity chromatography were applied to DEAE-Sephacel Chromatography and eluted by a stepwise increase in NaCl 0.15, 0.30 and 0.45 M in 0.01 M acetate buffer, pH 5.5. Sepharose 66-75 pappalysin 1 Homo sapiens 45-51 8978021-7 1996 Moreover, IE180 antigens obtained from lysates of CHX-reversed, PrV-infected cells by heparin/agarose affinity separation also stimulated specific proliferation of PBMNCs from MLV-vaccinated swine, as their proliferative responses were significantly higher than those of unvaccinated swine (p < or = 0.05). Sepharose 94-101 transcriptional regulator ICP4 Suid alphaherpesvirus 1 10-15 8537405-2 1995 The PDI-calreticulin complex can be dissociated by Zn(2+)-iminodiacetate-substituted Sepharose-agarose chromatography, suggesting that these interactions may be Zn2+-dependent. Sepharose 85-94 prolyl 4-hydroxylase subunit beta Homo sapiens 4-7 8537405-2 1995 The PDI-calreticulin complex can be dissociated by Zn(2+)-iminodiacetate-substituted Sepharose-agarose chromatography, suggesting that these interactions may be Zn2+-dependent. Sepharose 85-94 calreticulin Homo sapiens 8-20 8537405-2 1995 The PDI-calreticulin complex can be dissociated by Zn(2+)-iminodiacetate-substituted Sepharose-agarose chromatography, suggesting that these interactions may be Zn2+-dependent. Sepharose 95-102 prolyl 4-hydroxylase subunit beta Homo sapiens 4-7 8537405-2 1995 The PDI-calreticulin complex can be dissociated by Zn(2+)-iminodiacetate-substituted Sepharose-agarose chromatography, suggesting that these interactions may be Zn2+-dependent. Sepharose 95-102 calreticulin Homo sapiens 8-20 8547317-1 1995 Using monoclonal antibodies against Z-DNA three AluI restriction fragments of the human c-myc gene were previously found to form Z-DNA in agarose-embedded, metabolically active permeabilized nuclei. Sepharose 138-145 MYC proto-oncogene, bHLH transcription factor Homo sapiens 88-93 8530382-13 1995 PI9 was purified to homogeneity from the yeast cell lysate by a combination of heparin-agarose chromatography and Mono Q fast protein liquid chromatography and migrated as a single band in SDS-polyacrylamide gel electrophoresis with an apparent molecular mass of 42 kDa. Sepharose 87-94 serpin family B member 9 Homo sapiens 0-3 7498546-2 1995 RNA binding activity of the NS3 protein with an apparent dissociation constant of 2 x 10(-7) M was detected using a poly(U)-Sepharose resin. Sepharose 124-133 KRAS proto-oncogene, GTPase Homo sapiens 28-31 9161700-2 1996 The CR1 genotype was determined by a method based on polymerase chain reaction (PCR) amplification of the genomic DNA fragment of 1.8 kb separated by HindIII endonuclease digestion and agarose gel electrophoresis. Sepharose 185-192 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 4-7 8610756-2 1995 STUDY DESIGN: The alpha-fetoprotein level and the affinity of alpha-fetoprotein for concanavalin A Sepharose was measured between 8 and 11 weeks of gestation in the maternal serum and coelomic fluid of nine pregnancies complicated by an empty gestational sac and of 27 normal pregrancies. Sepharose 99-108 alpha fetoprotein Homo sapiens 62-79 8547663-8 1996 Furthermore, L-selectin IgG chimeric protein directly bound to type IV collagen-Sepharose column, and anti-L-selectin MoAb DREG56 inhibited the neutrophil adherence to type IV collagen-coated plastic by 51%. Sepharose 80-89 selectin L Homo sapiens 13-23 7503560-4 1995 Following activation, matrilysin was purified to > 95% homogeneity using a Sepharose-Pro-Leu-Gly-NHOH affinity column. Sepharose 78-87 matrix metallopeptidase 7 Homo sapiens 22-32 8770320-12 1995 Apolipoprotein (apo) B-100 on LDL was carried on polyacrylamide and agarose gel electrophoresis. Sepharose 68-75 apolipoprotein B Homo sapiens 0-26 7586732-5 1995 The presence of anti-idiotypic activity against anti-PDH fragments on Sepharose-bound IVIG [F(ab)2]. Sepharose 70-79 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 53-56 8556165-1 1995 Three biomimetic dye ligands bearing as a triazine-linked terminal moiety a carboxylated structure, which mimics substrates and inhibitors of L-lactate dehydrogenase (LDH), were immobilized on cross-linked agarose Ultrogel A6R. Sepharose 206-213 LDH Bos taurus 142-165 8547175-7 1995 The behaviour of 3 beta-HSD on a column of Heparin-Sepharose is modified by the presence of cyt. Sepharose 51-60 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase Bos taurus 17-27 8746633-3 1995 Approximately 40 micrograms of partially purified rHCII was routinely recovered from 50 ml of media after sequential heparin and Q-Sepharose affinity adsorption. Sepharose 131-140 serpin family D member 1 Rattus norvegicus 50-55 8772226-1 1995 Two forms of histidine-rich glycoprotein (HRG) were detected on SDS-PAGE by silver staining and immunoblotting after isolation of the protein from pooled plasma using immuno-affinity chromatography followed by chromatography with heparin-Sepharose. Sepharose 238-247 histidine rich glycoprotein Homo sapiens 13-40 8772226-1 1995 Two forms of histidine-rich glycoprotein (HRG) were detected on SDS-PAGE by silver staining and immunoblotting after isolation of the protein from pooled plasma using immuno-affinity chromatography followed by chromatography with heparin-Sepharose. Sepharose 238-247 histidine rich glycoprotein Homo sapiens 42-45 7488211-3 1995 LPL-sepharose affinity chromatography of [35S]O4 labeled HAEC proteins identified a 220-kDa proteoglycan. Sepharose 4-13 lipoprotein lipase Homo sapiens 0-3 7492310-3 1995 In the present study we isolated the proteins from bovine lung soluble fraction that bind to SP-A in a Ca(2+)-dependent manner using DEAE-Sephacel and SP-A-conjugated Sepharose 4B. Sepharose 167-176 pulmonary surfactant-associated protein A Bos taurus 93-97 7492310-3 1995 In the present study we isolated the proteins from bovine lung soluble fraction that bind to SP-A in a Ca(2+)-dependent manner using DEAE-Sephacel and SP-A-conjugated Sepharose 4B. Sepharose 167-176 pulmonary surfactant-associated protein A Bos taurus 151-155 8529649-2 1995 Bovine lung endothelin-B receptor has been isolated in good yield with a new procedure involving the use of endothelin-1 coupled to iminobiotin with a long spacer and avidin-agarose affinity chromatography. Sepharose 174-181 endothelin receptor type B Bos taurus 12-33 7488169-2 1995 By these purification steps, DNA polymerase and proliferating cell nuclear antigen (PCNA) were completely separated at the step of heparin-Sepharose CL-6B column chromatography. Sepharose 139-148 proliferating cell nuclear antigen Homo sapiens 48-82 7488169-2 1995 By these purification steps, DNA polymerase and proliferating cell nuclear antigen (PCNA) were completely separated at the step of heparin-Sepharose CL-6B column chromatography. Sepharose 139-148 proliferating cell nuclear antigen Homo sapiens 84-88 7592935-6 1995 The GDI-Rab5 complex can be captured using streptavidin-linked agarose beads. Sepharose 63-70 RAB5A, member RAS oncogene family Homo sapiens 8-12 7592941-6 1995 SAP-Sepharose specifically bound the 160-kDa fragment, suggesting that the central core of C4BP contains the binding site for SAP. Sepharose 4-13 amyloid P component, serum Homo sapiens 0-3 7592941-6 1995 SAP-Sepharose specifically bound the 160-kDa fragment, suggesting that the central core of C4BP contains the binding site for SAP. Sepharose 4-13 complement component 4 binding protein alpha Homo sapiens 91-95 7592941-6 1995 SAP-Sepharose specifically bound the 160-kDa fragment, suggesting that the central core of C4BP contains the binding site for SAP. Sepharose 4-13 amyloid P component, serum Homo sapiens 126-129 7488288-9 1995 Selective removal of HRES-1 antibodies from sera of HRES-1-seropositive/RNP-seropositive patients by absorption on recombinant HRES-1/glutathione-S-transferase-conjugated agarose beads had no effect on anti-RNP reactivities. Sepharose 171-178 HTLV-1 related endogenous sequence Homo sapiens 21-27 7488288-9 1995 Selective removal of HRES-1 antibodies from sera of HRES-1-seropositive/RNP-seropositive patients by absorption on recombinant HRES-1/glutathione-S-transferase-conjugated agarose beads had no effect on anti-RNP reactivities. Sepharose 171-178 glutathione S-transferase kappa 1 Homo sapiens 134-159 7579442-10 1995 Flow cytometry and DNA agarose gel electrophoresis showed that treated CD34+ cells accumulated in the G0/G1 region of the DNA histogram with no evidence of either differentiation or apoptosis. Sepharose 23-30 CD34 molecule Homo sapiens 71-75 7495305-9 1995 In addition, the mutant c-H-ras, but not c-myc, cooperated with the functional T antigen at 33 degrees C to allow growth in soft agarose of the CHST8 and CHST10-2.1 cell lines. Sepharose 129-136 Harvey rat sarcoma virus oncogene Mus musculus 24-31 7495305-9 1995 In addition, the mutant c-H-ras, but not c-myc, cooperated with the functional T antigen at 33 degrees C to allow growth in soft agarose of the CHST8 and CHST10-2.1 cell lines. Sepharose 129-136 carbohydrate sulfotransferase 8 Mus musculus 144-149 8787779-7 1995 As the final step of the purification, HMW-hCG was allowed to adsorb on a column of ATP-agarose and anti-hCG IgG-agarose, respectively. Sepharose 88-95 chorionic gonadotropin subunit beta 5 Homo sapiens 43-46 8748166-1 1995 alpha 1,3-Fucosyltransferase solubilized from human liver has been purified 40,000-fold to apparent homogeneity by a multistage process involving cation exchange chromatography on CM-Sephadex, hydrophobic interaction chromatography on Phenyl Sepharose, affinity chromatography on GDP-hexanolamine Sepharose and HPLC gel exclusion chromatography. Sepharose 242-251 fucosyltransferase 11 Homo sapiens 0-28 8732060-1 1995 The human placenta transferrin receptor was purified in the form of transferrin-transferrin receptor complex (Tf-TfR), and a monospecific polyclonal antibody against TfR was developed by a Tf-coupled Sepharose 4B affinity chromatography to remove the anti-Tf components in the antiserum. Sepharose 200-212 transferrin Homo sapiens 19-30 8732060-1 1995 The human placenta transferrin receptor was purified in the form of transferrin-transferrin receptor complex (Tf-TfR), and a monospecific polyclonal antibody against TfR was developed by a Tf-coupled Sepharose 4B affinity chromatography to remove the anti-Tf components in the antiserum. Sepharose 200-212 transferrin receptor Homo sapiens 166-169 7593230-1 1995 Spontaneously arising, TGF beta 1-resistant colonies were isolated directly from the soft agarose plates of MOSER human colon carcinoma cells grown in the presence of TGF beta 1 but in the absence of serum. Sepharose 90-97 transforming growth factor beta 1 Homo sapiens 23-33 8567753-7 1995 The addition of intact pro alpha 1(I)-N-propeptide at 25 micrograms/ml completely inhibited hsp47 binding to the gelatin-Sepharose. Sepharose 121-130 serine (or cysteine) peptidase inhibitor, clade H, member 1 Mus musculus 92-97 8600179-5 1995 The GST/re-Hst1 fusion protein was isolated from cell lysates by affinity chromatography on glutathione (GSH)-Sepharose and digested with cyanogen bromide to separate re-Hst1 from the GST fusion partner. Sepharose 110-119 glutathione S-transferase kappa 1 Homo sapiens 4-7 8600179-5 1995 The GST/re-Hst1 fusion protein was isolated from cell lysates by affinity chromatography on glutathione (GSH)-Sepharose and digested with cyanogen bromide to separate re-Hst1 from the GST fusion partner. Sepharose 110-119 fibroblast growth factor 4 Homo sapiens 11-15 8593245-0 1995 A 56,000 Mr phosphoseryl protein in PC12 cell lysates strongly associates with protein-A sepharose beads and was observed in immune complex kinase assays for PP60c-src. Sepharose 89-98 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 158-167 8593245-1 1995 Using an immune complex kinase assay to measure pp60c-src kinase activity, we have identified a 56,000 Mr protein (p56) from PC12 cell lysates that co-purified with pp60c-src by strong association with protein-A sepharose beads. Sepharose 212-221 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 48-57 8593245-1 1995 Using an immune complex kinase assay to measure pp60c-src kinase activity, we have identified a 56,000 Mr protein (p56) from PC12 cell lysates that co-purified with pp60c-src by strong association with protein-A sepharose beads. Sepharose 212-221 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 165-174 8593245-6 1995 The identify of p56 awaits further investigation but its appearance in immunoprecipitates of pp60c-src using protein-A sepharose beads is of interest but complicates the the interpretation of results from immune complex kinase assays in PC12 cells. Sepharose 119-128 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 93-102 8529028-1 1995 The catalytic subunit of protein phosphatase 2A (PP2Ac) was purified from Neurospora crassa extract by (NH4)2SO4-ethanol precipitation followed by DEAE-Sephacel, heparin-Sepharose, and MonoQ chromatography steps about 900-fold to a specific activity of 1200 U/g with a 2% yield. Sepharose 170-179 protein phosphatase 2 catalytic subunit alpha Homo sapiens 49-54 8567753-11 1995 GST-fusion protein constructs of residues 23-108 (NP1), 23-151 (NP2), and 23-178 (NP3) within the pro alpha 1 (I)- N-propeptide were coupled to Sepharose 4B and used as affinity beads for collection of hsp47 from 3T6 cell lysates. Sepharose 144-156 neuronal pentraxin 1 Mus musculus 50-53 8573469-1 1995 The Bacillus subtilis GroESL chaperonin was isolated by sucrose density gradient centrifugation and the constituent GroES and GroEL moieties were purified by electrophoresis in agarose. Sepharose 177-184 chaperonin GroES Escherichia coli 22-27 8590307-6 1995 The recombinant mutant MCP-3 was purified to homogeneity by adsorption on silicic acid, affinity chromatography on heparin-Sepharose, and reversed-phase HPLC. Sepharose 123-132 C-C motif chemokine ligand 7 Homo sapiens 23-28 8587495-4 1995 The charge of Epo in blood and concentrates of urine was determined by electrophoresis in 0.10% agarose suspension expressed as electrophoretic mobility. Sepharose 96-103 erythropoietin Homo sapiens 14-17 7592779-7 1995 VEGF from conditioned medium of v-raf transformed NIH 3T3 cells was partially purified by chromatography on heparin-Sepharose. Sepharose 116-125 vascular endothelial growth factor A Mus musculus 0-4 7491122-2 1995 The GST-rAPEN fusion was subsequently overexpressed in Escherichia coli, purified on glutathione-agarose affinity columns, and the purified protein tested for AP endonuclease activity. Sepharose 97-104 glutathione S-transferase kappa 1 Homo sapiens 4-7 8548657-2 1995 The affinity column was prepared by coupling CNBr-activated Sepharose with the segment of the 5"-untranslated region of encephalomyocarditis virus (EMCV) RNA previously shown to bind PTB. Sepharose 60-69 polypyrimidine tract binding protein 1 Homo sapiens 183-186 7559504-5 1995 Moreover, rapid inhibitors of cathepsin G and alpha-chymotrypsin suppressed neutrophil chemotaxis to the chemoattractants N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP) and zymosan-activated serum in multiple blind well assays and to fMLP in migration assays under agarose. Sepharose 271-278 cathepsin G Homo sapiens 30-41 7488053-2 1995 The activator was retained on a CaM-Sepharose column in the presence of Ca2+ and kinase assay of renatured gel revealed the 64 kDa molecule in the purified activator fraction to be autophosphorylated and to phosphorylate recombinant CaM kinase I in the presence of Ca2+/calmodulin. Sepharose 36-45 calmodulin 1 Rattus norvegicus 32-35 7578274-8 1995 Furthermore, depletion of the Ser-209 kinase activity with p13suc1-Sepharose and anti-p34cdc2 antiserum demonstrated conclusively that the isolated Ser-209 kinase is p34cdc2. Sepharose 67-76 cyclin dependent kinase 1 Homo sapiens 166-173 7575459-2 1995 Chromatography on antithrombin (AT)-Sepharose resulted in the separation of the 35S-labelled PGs into three fractions: PGs with no affinity for the gel (NA-PGs), PGs with low affinity (LA-PGs), and PGs with high affinity (HA-PGs) for antithrombin. Sepharose 36-45 serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Mus musculus 18-30 7487006-1 1995 A serine protease from the keratin-degrading Streptomyces pactum DSM 40530 was purified by casein agarose affinity chromatography. Sepharose 98-105 keratin Gallus gallus 27-34 8777055-5 1995 The pure scFv was coupled to CNBr-activated Sepharose 4B and compared the original monoclonal antibody (MAb) CB-Hep.1 in the immunoaffinity purification of a vaccine recombinant HBsAg (r-HBsAg). Sepharose 44-53 immunglobulin heavy chain variable region Homo sapiens 9-13 7583552-2 1995 HDL encompasses several apoA-I-containing particles that differ by size and show pre-beta- or alpha-mobility on agarose gel electrophoresis: pre-beta 1-LpA-I, pre-beta 2-LpA-I, pre-beta 3-LpA-I, alpha-LpA-I2, and alpha-LpA-I3. Sepharose 112-119 apolipoprotein A1 Homo sapiens 24-30 7558284-6 1995 All of the strains displayed a transferrin binding phenotype, and affinity isolation of receptor proteins with transferrin-conjugated Sepharose recovered Tbp1 and/or Tbp2 from 11 of 14 strains, including 2 of the nontypeable biotype IV strains. Sepharose 134-143 transferrin Homo sapiens 111-122 8581282-10 1995 Reverse-transcriptase polymerase chain reaction (RI-PCR) assays and subsequent product separation by agarose gel electrophoresis revealed single bands corresponding to the expected product sizes encoding cDNA for ETA (299 base pairs) and ETB (428 base pairs) (n = 3 different cultures). Sepharose 101-108 endothelin receptor type A Homo sapiens 213-216 8576934-7 1995 TIMP and MMP were further purified on collagen-Sepharose affinity column. Sepharose 47-56 TIMP metallopeptidase inhibitor 1 Homo sapiens 0-4 7558284-6 1995 All of the strains displayed a transferrin binding phenotype, and affinity isolation of receptor proteins with transferrin-conjugated Sepharose recovered Tbp1 and/or Tbp2 from 11 of 14 strains, including 2 of the nontypeable biotype IV strains. Sepharose 134-143 EGFLAM antisense RNA 3 Homo sapiens 154-158 7548166-5 1995 We also show that protamine was able to displace AChE bound to heparin-agarose. Sepharose 71-78 acetylcholinesterase Rattus norvegicus 49-53 8539777-5 1995 The labeling procedure preserved the main properties of native SHBG: interaction with the lectine concanavaline A-Sepharose, recognition by anti-hSHBG monoclonal antibody, and, although lower than in native SHBG, the binding affinity for 5 alpha-dihydrotestosterone. Sepharose 114-123 sex hormone-binding globulin Oryctolagus cuniculus 63-67 8539777-9 1995 [125I]rSHBG was detected by measurement of radioactivity either directly on serum or after fixation on concanavaline A-Sepharose. Sepharose 119-128 sex hormone binding globulin Rattus norvegicus 6-11 7574683-2 1995 The data showed that recombinant human gelatinase A (MMP-2) and B (MMP-9) were both specifically bound to asialofetuin and fetuin immobilized to activated agarose (affigel) with subsequent cleavage of the enzymes to lower molecular weight forms, which were likewise bound to asialofetuin/fetuin. Sepharose 155-162 matrix metallopeptidase 2 Homo sapiens 53-65 7557435-2 1995 The vectors, pGEX-GTH and pET-HTG, produce protein fused to glutathione S-transferase (GST) at the N- and C-termini, respectively, allowing one-step purification on glutathione-Sepharose. Sepharose 177-186 glutathione S-transferase kappa 1 Homo sapiens 60-85 7574683-2 1995 The data showed that recombinant human gelatinase A (MMP-2) and B (MMP-9) were both specifically bound to asialofetuin and fetuin immobilized to activated agarose (affigel) with subsequent cleavage of the enzymes to lower molecular weight forms, which were likewise bound to asialofetuin/fetuin. Sepharose 155-162 matrix metallopeptidase 9 Homo sapiens 67-72 7557435-2 1995 The vectors, pGEX-GTH and pET-HTG, produce protein fused to glutathione S-transferase (GST) at the N- and C-termini, respectively, allowing one-step purification on glutathione-Sepharose. Sepharose 177-186 glutathione S-transferase kappa 1 Homo sapiens 87-90 7673143-6 1995 With the exception of L10R, all of the mutants were successfully expressed in Escherichia coli and purified by affinity chromatography on PLP-Sepharose. Sepharose 142-151 proteolipid protein 1 Homo sapiens 138-141 7669046-6 1995 The TC were then solubilized with CHAPS and the complex of RyR.GST/FKBP12 was specifically adsorbed by glutathione Sepharose 4B and then eluted with glutathione. Sepharose 115-127 ryanodine receptor 1 Homo sapiens 59-62 7673175-15 1995 Furthermore, under mild conditions NH2OH caused dissociation and inactivation of approximately 50% of the total ASGP-Rs (State 1 and State 2) that were prebound to ASOR-Sepharose, whereas the same treatment caused dissociation of only < 20% of State 1 ASGP-Rs from such preformed complexes. Sepharose 169-178 mucin 4, cell surface associated Rattus norvegicus 112-116 7669046-6 1995 The TC were then solubilized with CHAPS and the complex of RyR.GST/FKBP12 was specifically adsorbed by glutathione Sepharose 4B and then eluted with glutathione. Sepharose 115-127 FKBP prolyl isomerase 1A Homo sapiens 67-73 7544379-6 1995 Eosinophil degranulation induced by IgG covalently coupled to Sepharose 4B beads was also inhibited by mAb to CD18. Sepharose 62-74 integrin subunit beta 2 Homo sapiens 110-114 8572575-9 1995 Agarose gel electrophoresis revealed characteristic DNA laddering only at 35 microM dose of VP 16. Sepharose 0-7 host cell factor C1 Homo sapiens 92-97 7592126-3 1995 Esterase electrophoretic typing, based on esterase activity against seven synthetic substrates after polyacrylamide-agarose gel electrophoresis, led to the description of 12 electrophoretic types, two of which were predominant with 60 and 20 strains respectively. Sepharose 116-123 AT695_RS05650 Staphylococcus aureus 0-8 9223007-5 1995 Free rat cpn10 was released from avidin-agarose column with 5% aqueous triethylamine and after desalting by RP-HPLC gave 9.9% recovery. Sepharose 40-47 heat shock protein family E (Hsp10) member 1 Rattus norvegicus 9-14 8596433-2 1995 By using the "two-hybrid system" and co-purification experiments on glutathione-agarose beads, we have shown that Slt2p interacts in vivo and in vitro with both Mkk1p and Mkk2p, thus confirming a previous suggestion based on epistasis experiments of the corresponding genes. Sepharose 80-87 mitogen-activated serine/threonine-protein kinase SLT2 Saccharomyces cerevisiae S288C 114-119 7544912-3 1995 The alpha 2M/pMBP-28 complexes was isolated by PEG-precipitation and affinity chromatography on mannan-Sepharose, protein A-Sepharose and anti-IgM Sepharose. Sepharose 103-112 alpha-2-macroglobulin Homo sapiens 4-12 7544912-3 1995 The alpha 2M/pMBP-28 complexes was isolated by PEG-precipitation and affinity chromatography on mannan-Sepharose, protein A-Sepharose and anti-IgM Sepharose. Sepharose 103-112 progesterone receptor membrane component 2 Homo sapiens 13-17 7544912-3 1995 The alpha 2M/pMBP-28 complexes was isolated by PEG-precipitation and affinity chromatography on mannan-Sepharose, protein A-Sepharose and anti-IgM Sepharose. Sepharose 124-133 alpha-2-macroglobulin Homo sapiens 4-12 7544912-3 1995 The alpha 2M/pMBP-28 complexes was isolated by PEG-precipitation and affinity chromatography on mannan-Sepharose, protein A-Sepharose and anti-IgM Sepharose. Sepharose 124-133 progesterone receptor membrane component 2 Homo sapiens 13-17 7544912-3 1995 The alpha 2M/pMBP-28 complexes was isolated by PEG-precipitation and affinity chromatography on mannan-Sepharose, protein A-Sepharose and anti-IgM Sepharose. Sepharose 124-133 alpha-2-macroglobulin Homo sapiens 4-12 7544912-3 1995 The alpha 2M/pMBP-28 complexes was isolated by PEG-precipitation and affinity chromatography on mannan-Sepharose, protein A-Sepharose and anti-IgM Sepharose. Sepharose 124-133 progesterone receptor membrane component 2 Homo sapiens 13-17 7544912-4 1995 The occurrence of alpha 2M/pMBP-28 complexes was further indicated by crossed immunoelectrophoresis and by use of an anti-alpha 2M affinity column and chelating Sepharose loaded with Zn2+. Sepharose 161-170 alpha-2-macroglobulin Homo sapiens 18-26 7673405-7 1995 FGF-2 was extracted from serum and amniotic fluid by heparin-Sepharose affinity chromatography and subjected to Western blot analysis or quantified by specific RIA. Sepharose 61-70 fibroblast growth factor 2 Homo sapiens 0-5 7544350-7 1995 Immunoaffinity chromatography of detergent extracts of rat synaptic plasma membranes on anti-phosphotyrosine antibody-agarose showed that the NR2A and NR2B subunits but not the NR1 subunit are tyrosine-phosphorylated. Sepharose 118-125 glutamate ionotropic receptor NMDA type subunit 2A Rattus norvegicus 142-146 7654238-2 1995 In order to detail the DNA-binding specificity of this protein, a TTF-1HD-Sepharose column chromatography was used. Sepharose 74-83 transcription termination factor 1 Homo sapiens 66-71 7654695-7 1995 When purified PCR178 was coupled to a cyanogen bromide-activated Sepharose column, bovine hsp70 bound to the column and was eluted with MgATP. Sepharose 65-74 heat shock 70 kDa protein 1B Bos taurus 90-95 7556138-5 1995 After the reaction, only the GST-P complexed with the glutathione analogue was prepared with glutathione-immobilized agarose. Sepharose 117-124 glutathione S-transferase pi 1 Rattus norvegicus 29-34 7636265-4 1995 That the 120-kDa phosphoprotein coimmunoprecipitated by anti-lyn Abs is the beta subunit of GM-CSFR was confirmed in the immunoprecipitates (IP) of M-07e cells with the use of an agarose-conjugated anti-p-tyr mAb. Sepharose 179-186 LYN proto-oncogene, Src family tyrosine kinase Homo sapiens 61-64 8535285-0 1995 Isolation of ERp72 from guinea pig term placentae using heparin Sepharose affinity chromatography. Sepharose 64-73 protein disulfide isomerase family A member 4 Homo sapiens 13-18 8535287-3 1995 We demonstrated, using a sepharose-bead-Tf complex, the rapid recycling of unoccupied internal transferrin receptors was correlated with ligand binding to surface receptors. Sepharose 25-34 transferrin Homo sapiens 95-106 7578576-1 1995 Previous studies have demonstrated that fibronectin immobilized on BrCN-activated agarose forms a complex with soluble myeloperoxidase. Sepharose 82-89 fibronectin 1 Homo sapiens 40-51 7578576-1 1995 Previous studies have demonstrated that fibronectin immobilized on BrCN-activated agarose forms a complex with soluble myeloperoxidase. Sepharose 82-89 myeloperoxidase Homo sapiens 119-134 7578576-3 1995 The thermodynamic characteristics of the myeloperoxidase interaction with fibronectin-gelatin-agarose have been established. Sepharose 94-101 myeloperoxidase Homo sapiens 41-56 7578576-3 1995 The thermodynamic characteristics of the myeloperoxidase interaction with fibronectin-gelatin-agarose have been established. Sepharose 94-101 fibronectin 1 Homo sapiens 74-85 8555986-2 1995 The biological activity of vWF was measured by the ristocetin cofactor assay and its multimeric structure was assessed by Western immunoblotting after SDS-agarose gel electrophoresis. Sepharose 155-162 von Willebrand factor Homo sapiens 27-30 7544912-4 1995 The occurrence of alpha 2M/pMBP-28 complexes was further indicated by crossed immunoelectrophoresis and by use of an anti-alpha 2M affinity column and chelating Sepharose loaded with Zn2+. Sepharose 161-170 progesterone receptor membrane component 2 Homo sapiens 27-31 7544912-7 1995 Fractionation of pMBP-containing D-mannose eluate from mannan-Sepharose on Superose 6 showed two protein peaks which reacted with anti-C1 s antibodies in ELISA, one of about 650-800 kDa, which in addition contained pMBP-28 and anti-alpha 2M reactive material, the other with an M(r) of 100-150 kDa. Sepharose 62-71 progesterone receptor membrane component 2 Homo sapiens 17-21 8527931-9 1995 Passage of the cell-free culture medium over an affinity column of 5-formyltetrahydrofolate-Sepharose provided 10-FTHFDH that was more than 95% pure. Sepharose 92-101 aldehyde dehydrogenase 1 family, member L1 Rattus norvegicus 111-120 8529603-4 1995 Measurements of the immunoglobulin IgE in dot blots and in blots after sodium dodecyl sulfate (SDS) electrophoresis under nonreducing conditions in agarose gels are described. Sepharose 148-155 immunoglobulin heavy constant epsilon Homo sapiens 35-38 7631157-5 1995 The product purified by Protein G-Sepharose was identified as authentic MAdCAM-1-Fc by the anti-MAdCAM-1 monoclonal antibody (MoAb) MECA-367 using Western blot and ELISA analysis. Sepharose 34-43 mucosal vascular addressin cell adhesion molecule 1 Homo sapiens 72-80 7495171-1 1995 Polymorphism of prochymosin was observed in individual calf abomasa, using agarose gel electrophoresis followed by detection of proteolytic activity. Sepharose 75-82 chymosin Bos taurus 16-27 7495171-3 1995 Four distinct prochymosins were found and, according to their decreasing electrophoretic mobility in alkaline agarose gel, termed as prochymosin A, D, B and C which occurred singly and in pairs (then with equal proteolytic activities of both components). Sepharose 110-117 chymosin Bos taurus 14-25 7629119-5 1995 Mutant GST fusion proteins that contain a single amino acid change (Y392S, Y392F, and Y392W) in the AIDA along with control GST were coupled to glutathione-Sepharose beads to form affinity beads. Sepharose 156-165 glutathione S-transferase kappa 1 Homo sapiens 7-10 7629119-5 1995 Mutant GST fusion proteins that contain a single amino acid change (Y392S, Y392F, and Y392W) in the AIDA along with control GST were coupled to glutathione-Sepharose beads to form affinity beads. Sepharose 156-165 axin interactor, dorsalization associated Homo sapiens 100-104 7629119-11 1995 Also, beta subunits from solubilized skeletal muscle triads can be affinity-purified using AIDA CNBr-Sepharose. Sepharose 101-110 axin interactor, dorsalization associated Homo sapiens 91-95 7622504-4 1995 SF9 cells infected with vAc-DTRB3 expressed functional DTR, which could be precipitated from the solubilized membrane fraction of infected cells with Sepharose-immobilized diphtheria toxin. Sepharose 150-159 heparin binding EGF like growth factor Homo sapiens 28-31 7559467-5 1995 Furthermore, alpha 8 beta 1 binds to both fibronectin- and vitronectin-Sepharose and can be specifically eluted from either matrix protein by the arginine-glycine-aspartic acid (RGD)-containing peptide, GRGDSP. Sepharose 71-80 vitronectin Homo sapiens 59-70 7622864-8 1995 A production system consisting of transfected 293-EBNA cells cultured in serum free medium followed by protein G-Sepharose chromatography of the conditioned medium was found to be sufficient for the rapid production of purified chimeric Fab. Sepharose 113-122 FA complementation group B Homo sapiens 237-240 7608216-3 1995 Affinity-purified receptor isolated by adsorption to phosphomannan-agarose and elution with mannose 6-phosphate contained nearly stoichiometric amounts of bound 7.5-kDa IGF-II. Sepharose 67-74 insulin like growth factor 2 Homo sapiens 169-175 7625844-9 1995 The CDP effect on glycogenin further allowed the development of an improved procedure for the purification of this enzyme, in which specific elution of an affinity matrix (UDP-glucuronic acid-agarose) was carried out with CDP as the eluant. Sepharose 192-199 cut like homeobox 1 Homo sapiens 4-7 8578949-2 1995 Tyrosinase was purified from 100 microl serum by adsorption to concanavalin A sepharose, the tyrosinase adsorbed to the gel being separated from other components by centrifugation. Sepharose 78-87 tyrosinase Homo sapiens 0-10 7580117-6 1995 HSA or BSA linked to Sepharose was incubated with a 15, 20, or 24 mer S-ODN followed by the addition of selected drugs known to be highly protein bound (nifedipine, warfarin, midazolam, probenecid, indomethacin, and mitoxantrone). Sepharose 21-30 albumin Homo sapiens 7-10 7647008-2 1995 The predominant species of factor Xa-ATIII detected after plasma and plasma to which factor Xa had been added were gel filtered on Sephadex G-200 and Sepharose 4B had apparent M(r) > 200,000, in which factor Xa-ATIII was associated with vitronectin. Sepharose 150-159 coagulation factor X Homo sapiens 27-36 7647008-2 1995 The predominant species of factor Xa-ATIII detected after plasma and plasma to which factor Xa had been added were gel filtered on Sephadex G-200 and Sepharose 4B had apparent M(r) > 200,000, in which factor Xa-ATIII was associated with vitronectin. Sepharose 150-159 serpin family C member 1 Homo sapiens 37-42 7600689-0 1995 Apolipoprotein E genotyping on agarose gels. Sepharose 31-38 apolipoprotein E Homo sapiens 0-16 7545566-4 1995 The results showed that albumin, lactoferrin, transferrin, and lysozyme could--but serum prealbumin, IgA, carboxymethyl cellulose (CMC), and Sepharose 4B-purified porcine stomach mucin (PSM) could not--bind rose bengal. Sepharose 141-153 serotransferrin Oryctolagus cuniculus 46-57 7593463-5 1995 The following results indicate that this suppression is mediated by IgG: (1) IVIG preparations, which consist mainly of IgG, suppressed the IgE synthesis from IL-4-stimulated PBMC in a dose-dependent way; (2) when HS was fractionated by protein G sepharose or anti-IgG sepharose, the eluate fractions (containing IgG), but not the effluent fractions (void of IgG) suppressed IgE synthesis, whereas the opposite was found when HS was fractionated by FCS-coupled sepharose. Sepharose 247-256 interleukin 4 Homo sapiens 159-163 7593463-5 1995 The following results indicate that this suppression is mediated by IgG: (1) IVIG preparations, which consist mainly of IgG, suppressed the IgE synthesis from IL-4-stimulated PBMC in a dose-dependent way; (2) when HS was fractionated by protein G sepharose or anti-IgG sepharose, the eluate fractions (containing IgG), but not the effluent fractions (void of IgG) suppressed IgE synthesis, whereas the opposite was found when HS was fractionated by FCS-coupled sepharose. Sepharose 269-278 interleukin 4 Homo sapiens 159-163 7593463-5 1995 The following results indicate that this suppression is mediated by IgG: (1) IVIG preparations, which consist mainly of IgG, suppressed the IgE synthesis from IL-4-stimulated PBMC in a dose-dependent way; (2) when HS was fractionated by protein G sepharose or anti-IgG sepharose, the eluate fractions (containing IgG), but not the effluent fractions (void of IgG) suppressed IgE synthesis, whereas the opposite was found when HS was fractionated by FCS-coupled sepharose. Sepharose 269-278 interleukin 4 Homo sapiens 159-163 7791777-6 1995 pOTC import was also decreased when the precursor was synthesized in the lysate depleted for hsc70 by treatment with hsc70 antibody-conjugated Sepharose. Sepharose 143-152 heat shock protein family A (Hsp70) member 8 Homo sapiens 93-98 7791777-6 1995 pOTC import was also decreased when the precursor was synthesized in the lysate depleted for hsc70 by treatment with hsc70 antibody-conjugated Sepharose. Sepharose 143-152 heat shock protein family A (Hsp70) member 8 Homo sapiens 117-122 8532625-3 1995 Polymerase chain reaction (PCR) amplification of exon 10 and parts of the flanking introns of the gene encoding the beta 3 chain of laminin 5 (LAMB3) and subsequent analysis by agarose gel electrophoresis showed a more slowly migrating band in the affected fetus compared with the normal control. Sepharose 177-184 laminin subunit beta 3 Homo sapiens 143-148 7794926-9 1995 Heparin, which binds to CKII alpha, inhibited the binding of CKII to HSP90-Sepharose. Sepharose 75-84 casein kinase 2 alpha 2 Homo sapiens 24-34 7794926-9 1995 Heparin, which binds to CKII alpha, inhibited the binding of CKII to HSP90-Sepharose. Sepharose 75-84 casein kinase 2 alpha 1 Homo sapiens 24-28 7794926-9 1995 Heparin, which binds to CKII alpha, inhibited the binding of CKII to HSP90-Sepharose. Sepharose 75-84 heat shock protein 90 alpha family class A member 1 Homo sapiens 69-74 7612663-3 1995 Chymase was purified from human skin by high salt extraction, cetylpyridinium chloride precipitation, heparin agarose affinity chromatography and gel filtration. Sepharose 110-117 chymase 1 Homo sapiens 0-7 7791217-6 1995 Analysis of endonuclease III-treated dihydrothymidine containing plasmid DNA by agarose gel electrophoresis indicated that the enzyme generated only single-strand breaks when the base damage was set one and three base-pairs apart, and only slowly introduced double-strand breaks in the other substrates. Sepharose 80-87 endonuclease III Escherichia coli 12-28 7794894-8 1995 We next developed a rapid purification method for bacterial recombinant MyoD-bHLH domain by affinity chromatography using a calmodulin-Sepharose column and investigated the phosphorylation of that peptide by PKC and its interactions with calmodulin and S100a. Sepharose 135-144 basic helix-loop-helix family, member a15 Rattus norvegicus 77-81 7797516-6 1995 (ii) Phosphorylation of residue Ser488 influences the affinity of RK for heparin-Sepharose only moderately, whereas Thr489 and Lys491 are important for this interaction. Sepharose 81-90 G protein-coupled receptor kinase 1 Homo sapiens 66-68 7782349-4 1995 Bovine kidney eIF-4E enhanced up to 1.8-fold globin synthesis in m7GTP-Sepharose-treated reticulocyte lysates. Sepharose 71-80 eukaryotic translation initiation factor 4E Bos taurus 14-20 7601155-4 1995 After ligation, the covalently closed substrate was used to follow an 1800-fold purification of the mouse X-solvase (EMX1) from crude nuclear extracts by chromatography on DEAE-cellulose, MonoQ and heparin-Sepharose. Sepharose 206-215 empty spiracles homeobox 1 Mus musculus 117-121 7779780-7 1995 The Nter domain of apo(a) was purified as a soluble protein in a two-step procedure which involved sequential use of a heparin-Sepharose column and a lysine-Sepharose column. Sepharose 127-136 lipoprotein(a) Homo sapiens 19-25 7779780-7 1995 The Nter domain of apo(a) was purified as a soluble protein in a two-step procedure which involved sequential use of a heparin-Sepharose column and a lysine-Sepharose column. Sepharose 157-166 lipoprotein(a) Homo sapiens 19-25 7779780-8 1995 The Cter domain of apo(a), which remained in disulfide linkage with apo B100 of Lp(a), was isolated as a lipoprotein particle by a combination of chromatographic steps on heparin-Sepharose and Q-Sepharose columns. Sepharose 179-188 lipoprotein(a) Homo sapiens 19-25 7779780-8 1995 The Cter domain of apo(a), which remained in disulfide linkage with apo B100 of Lp(a), was isolated as a lipoprotein particle by a combination of chromatographic steps on heparin-Sepharose and Q-Sepharose columns. Sepharose 195-204 lipoprotein(a) Homo sapiens 19-25 7768885-3 1995 An activity to activate a recombinant rat SAPK alpha was detected in extracts obtained from rat fibroblastic 3Y1 cells exposed to hyperosmolar media and was resolved into unadsorbed and adsorbed fractions on Q-Sepharose chromatography. Sepharose 210-219 mitogen-activated protein kinase 9 Rattus norvegicus 42-52 7768901-3 1995 To identify ligands specific for gp330 and relevant to its localization on epithelia such as in the mammary gland, gp330-Sepharose affinity chromatography was performed. Sepharose 121-130 LDL receptor related protein 2 Homo sapiens 33-38 7768901-3 1995 To identify ligands specific for gp330 and relevant to its localization on epithelia such as in the mammary gland, gp330-Sepharose affinity chromatography was performed. Sepharose 121-130 LDL receptor related protein 2 Homo sapiens 115-120 7768945-2 1995 Mammalian chaperonin homolog (HSP60) was purified from porcine livers cytosol using a tandem ATP-Sepharose column and Mono Q column chromatography. Sepharose 97-106 heat shock protein family D (Hsp60) member 1 Homo sapiens 30-35 7576231-1 1995 Cathepsin G was isolated from granules of rabbit bloodstream leukocytes and purified to apparent homogeneity by a multi-step procedure consisting of ammonium sulphate precipitation, affinity chromatography on elastin-Sepharose, and finally by ion-exchange chromatography on a CM-52 column. Sepharose 217-226 cathepsin G Homo sapiens 0-11 7757970-2 1995 We produced a recombinant MAGE-3 gene product by expression cloning of the entire reading frame in the context of a fusion protein characterized by a 10-histidine tail, allowing purification by metal chelation on a nickel Sepharose column. Sepharose 222-231 MAGE family member A3 Homo sapiens 26-32 7579799-7 1995 In addition, L-KT9 and DH3 cells bound to asialo-fetuin-coupled Sepharose (ASF-Sepharose) and this binding was significantly inhibited by pre-treatment of cells with anti-CD23, but not with anti-CD21 or other anti-adhesion molecules. Sepharose 64-73 Fc epsilon receptor II Homo sapiens 171-175 7579799-7 1995 In addition, L-KT9 and DH3 cells bound to asialo-fetuin-coupled Sepharose (ASF-Sepharose) and this binding was significantly inhibited by pre-treatment of cells with anti-CD23, but not with anti-CD21 or other anti-adhesion molecules. Sepharose 64-73 complement C3d receptor 2 Homo sapiens 195-199 7579799-7 1995 In addition, L-KT9 and DH3 cells bound to asialo-fetuin-coupled Sepharose (ASF-Sepharose) and this binding was significantly inhibited by pre-treatment of cells with anti-CD23, but not with anti-CD21 or other anti-adhesion molecules. Sepharose 79-88 Fc epsilon receptor II Homo sapiens 171-175 7579799-7 1995 In addition, L-KT9 and DH3 cells bound to asialo-fetuin-coupled Sepharose (ASF-Sepharose) and this binding was significantly inhibited by pre-treatment of cells with anti-CD23, but not with anti-CD21 or other anti-adhesion molecules. Sepharose 79-88 complement C3d receptor 2 Homo sapiens 195-199 7768617-5 1995 Affinity chromatography on hemopexin-Sepharose 4B of detergent-solubilized membranes from Hib strain 760705 results in the copurification of three proteins with molecular masses of 57, 38, and 29 kDa. Sepharose 37-46 hemopexin Homo sapiens 27-36 7540194-5 1995 After incubation with a Jurkat cell lysate, the GST-Nef constructs immobilized on glutathione-agarose beads bound to cellular kinase(s) and were phosphorylated at three sites in vitro: one on threonine at position 15, one on serine between residues 1 and 35, and one on threonine between residues 36 and 86. Sepharose 94-101 S100 calcium binding protein B Homo sapiens 52-55 8538681-5 1995 DHFR-TS was purified by methotrexate-Sepharose chromatography. Sepharose 37-46 dihydrofolate reductase Homo sapiens 0-4 7663153-4 1995 Following induction and cell disruption, a protein composed of Schistosoma japonicum glutathione S-transferase and E. coli thioredoxin was obtained in soluble form and purified by affinity chromatography on agarose columns bearing immobilized glutathione. Sepharose 207-214 thioredoxin Bos taurus 123-134 7539971-7 1995 The result was the same irrespective of whether poly(U)-Sepharose or viral mRNA was used, indicating that ssRNA-binding by NS2 is nonspecific. Sepharose 56-65 NS2 Homo sapiens 123-126 7744732-7 1995 The P-gp content of detergent-solubilized plasma membrane from MEL/VCR-6 cells could be appreciably depleted by treating this material with CLM-Sepharose beads as shown by SDS-polyacrylamide gel electrophoresis (PAGE) and Western blotting with anti-P-gp antibody (C219) before and after CLM-Sepharose treatment. Sepharose 144-153 phosphoglycolate phosphatase Mus musculus 4-8 7539816-4 1995 FGF-2 was extracted from maternal serum (MS), cord serum (CS), and AF by heparin-Sepharose affinity chromatography and subjected to Western blot analysis or quantified by specific RIA. Sepharose 81-90 fibroblast growth factor 2 Homo sapiens 0-5 7766603-2 1995 Fluorescence polarization binding assays of TR-CB7 with pFn and the 40-kDa GBF yielded progressively higher Kd"s with increased time of exposure to 280-nm light at 25 degrees C. Binding of nonirradiated and irradiated pFn and fragments to gelatin-Sepharose correlated with the polarization data, confirming diminished gelatin binding following exposure to 280-nm light. Sepharose 247-256 Kruppel like factor 6 Homo sapiens 75-78 7766684-2 1995 In the course of our investigation, we detected a serum protein that co-eluted with IgA from jacalin-agarose affinity column. Sepharose 101-108 CD79a molecule Homo sapiens 84-87 7744732-7 1995 The P-gp content of detergent-solubilized plasma membrane from MEL/VCR-6 cells could be appreciably depleted by treating this material with CLM-Sepharose beads as shown by SDS-polyacrylamide gel electrophoresis (PAGE) and Western blotting with anti-P-gp antibody (C219) before and after CLM-Sepharose treatment. Sepharose 144-153 phosphoglycolate phosphatase Mus musculus 249-253 7612053-1 1995 Low-anticoagulant heparin (LA-heparin) obtained by affinity chromatography on antithrombin III Sepharose inhibits the proliferation of cultured arterial smooth muscle cells in an in vitro bioassay system as effectively as standard heparin. Sepharose 95-104 serpin family C member 1 Rattus norvegicus 78-94 7744732-7 1995 The P-gp content of detergent-solubilized plasma membrane from MEL/VCR-6 cells could be appreciably depleted by treating this material with CLM-Sepharose beads as shown by SDS-polyacrylamide gel electrophoresis (PAGE) and Western blotting with anti-P-gp antibody (C219) before and after CLM-Sepharose treatment. Sepharose 291-300 phosphoglycolate phosphatase Mus musculus 4-8 7744733-4 1995 Peptides containing such sequences (P-(145-159) and P-(249-262)) were able to release asymmetric AChE bound to heparin-agarose. Sepharose 119-126 acetylcholinesterase (Cartwright blood group) Homo sapiens 97-101 7794038-5 1995 The presence of PK was assessed by incubation with soya bean trypsin inhibitor (SBTI), and by adsorption with anti-PK antibody linked to Sepharose. Sepharose 137-146 kallikrein B1 Homo sapiens 16-18 7794038-5 1995 The presence of PK was assessed by incubation with soya bean trypsin inhibitor (SBTI), and by adsorption with anti-PK antibody linked to Sepharose. Sepharose 137-146 kallikrein B1 Homo sapiens 115-117 7794038-8 1995 RESULTS: Amidase activity was demonstrated in synovial fluid pools and shown to be inhibited completely by SBTI, and removed by prior treatment with anti-PK Sepharose. Sepharose 157-166 kallikrein B1 Homo sapiens 154-156 7794038-12 1995 Most of the measured immunoreactivity was removed by adsorption with anti-PK antibody linked to Sepharose. Sepharose 96-105 kallikrein B1 Homo sapiens 74-76 7658163-2 1995 When PLA2 from the venom of Agkistrodon piscivorus piscivorus was pretreated with 4-nitro-3-octanoyl-oxybenzoic acid to acylate epsilon-amino groups of two lysines (Lys-7 and Lys-10) and the resulting acylated enzyme was covalently coupled onto carbonyldiimidazole-activated cross-linked agarose beads, the immobilized acylated enzyme showed high retention of activity toward various aggregated phospholipids. Sepharose 288-295 phospholipase A2 group IB Homo sapiens 5-9 7750206-2 1995 In this report we show that EGF promotes the malignant behavior of the Moser cells in vitro in terms of growth in soft agarose and invasion of Matrigel-coated porous membranes. Sepharose 119-126 epidermal growth factor Homo sapiens 28-31 7750206-3 1995 Expressing antisense EGFR RNA in the Moser cells (through transfection with an inducible antisense EGFR expression vector) downmodulated the expression of cell surface EGFR and EGFR mRNA with a concurrent inhibition of growth in soft agarose and invasion of Matrigel-coated membranes. Sepharose 234-241 epidermal growth factor receptor Homo sapiens 21-25 7750206-3 1995 Expressing antisense EGFR RNA in the Moser cells (through transfection with an inducible antisense EGFR expression vector) downmodulated the expression of cell surface EGFR and EGFR mRNA with a concurrent inhibition of growth in soft agarose and invasion of Matrigel-coated membranes. Sepharose 234-241 epidermal growth factor receptor Homo sapiens 99-103 7750206-3 1995 Expressing antisense EGFR RNA in the Moser cells (through transfection with an inducible antisense EGFR expression vector) downmodulated the expression of cell surface EGFR and EGFR mRNA with a concurrent inhibition of growth in soft agarose and invasion of Matrigel-coated membranes. Sepharose 234-241 epidermal growth factor receptor Homo sapiens 99-103 7750206-3 1995 Expressing antisense EGFR RNA in the Moser cells (through transfection with an inducible antisense EGFR expression vector) downmodulated the expression of cell surface EGFR and EGFR mRNA with a concurrent inhibition of growth in soft agarose and invasion of Matrigel-coated membranes. Sepharose 234-241 epidermal growth factor receptor Homo sapiens 99-103 7635280-4 1995 Our orthogonal field agarose gel electrophoresis analysis of chromosomal DNA from strains with KRB1 shows a novel band of approximately 250 kb. Sepharose 21-28 ribosomal 60S subunit protein L8B Saccharomyces cerevisiae S288C 95-99 7730030-7 1995 Both the 70-to 85-kDa and the 55-kDa proteins were precipitated by FGF-2 heparin-Sepharose but not by heparin-Sepharose alone, suggesting that the interaction was dependent on the presence of FGF-2. Sepharose 81-90 fibroblast growth factor 2 Homo sapiens 67-72 7730030-7 1995 Both the 70-to 85-kDa and the 55-kDa proteins were precipitated by FGF-2 heparin-Sepharose but not by heparin-Sepharose alone, suggesting that the interaction was dependent on the presence of FGF-2. Sepharose 81-90 fibroblast growth factor 2 Homo sapiens 192-197 7658169-1 1995 Phospholipid transfer protein (PLTP) from pig plasma was purified to homogeneity using ultracentrifugation and a combination of hydrophobic-, heparin-Sepharose-, and anti-pig-PLTP-affinity chromatography techniques. Sepharose 150-159 PLTP Sus scrofa 31-35 7730370-6 1995 Binding of FACC gamma 1 to protein A-agarose and incubation with radiolabeled cell lysates identified three polypeptides with molecular masses of 65, 50, and 35 kDa that were also detected on immunoblots probed with the purified FACC gamma 1 polypeptide. Sepharose 37-44 FA complementation group C Homo sapiens 11-15 7627554-4 1995 RESULTS: We have identified importin 90, a 90 kD second subunit that dissociates from importin 60 during affinity chromatography on nickel (II)-nitrolotriacetic acid-Sepharose, a technique that was originally used to purify importin 60. Sepharose 166-175 karyopherin (importin) beta 1 S homeolog Xenopus laevis 28-39 7721854-10 1995 AKAP100 was detected in preparations of RII purified from L6P cell extracts by cAMP-agarose affinity chromatography. Sepharose 84-91 A-kinase anchoring protein 6 Homo sapiens 0-7 7482433-1 1995 We have developed a simple, rapid assay method to measure remnant-like lipoproteins by using an immunoaffinity gel mixture of anti apo B-100 and apoA-1 antibodies to Sepharose 4B. Sepharose 166-178 apolipoprotein A1 Homo sapiens 145-151 7720853-1 1995 The form of protein phosphatase-1 associated with hepatic glycogen (PP1G) was purified to near homogeneity from rat liver by affinity chromatography on microcystin-Sepharose and gel-filtration. Sepharose 164-173 protein phosphatase 1 catalytic subunit gamma Rattus norvegicus 68-72 7726560-3 1995 Structural analysis of the GPI glycan of NCAM, which was purified from C2C12 myotubes after metabolic labeling with [3H]inositol, was performed by sequential exoglycosidase digestion and Wistaria floribunda agglutinin-agarose column chromatography. Sepharose 218-225 neural cell adhesion molecule 1 Mus musculus 41-45 7794814-11 1995 Also, addition of TGF-beta 1 to NSCLC cells inhibited colony formation of some of these cells in soft agarose in a dose-dependent manner. Sepharose 102-109 transforming growth factor beta 1 Homo sapiens 18-28 9049329-2 1995 The ability of GST-Nef to act as a substrate for cellular kinases in vitro was examined by incubation of purified GST-Nef fusion proteins, immobilized on glutathione-agarose beads, with cytoplasmic extracts from a number of human cell lines. Sepharose 166-173 S100 calcium binding protein B Homo sapiens 19-22 7547690-7 1995 When culture medium was analyzed with a radioimmunoassay for human placenta Trx, the MP6 clone was shown to release 30-50 ng/ml per million cells during 24 h. The B cell stimulatory activity of the MP6 medium was removed by Sepharose-bound anti-human placenta Trx IgG and activity was recovered by elution from the antibodies. Sepharose 224-233 thioredoxin Homo sapiens 260-263 7620338-1 1995 We have recently described a new method for measurement of tyrosinase activity in small amounts of human serum (100 microliters), where the purification of tyrosinase is obtained by adsorption of the enzyme to concanavalin A sepharose. Sepharose 225-234 tyrosinase Homo sapiens 59-69 7620338-1 1995 We have recently described a new method for measurement of tyrosinase activity in small amounts of human serum (100 microliters), where the purification of tyrosinase is obtained by adsorption of the enzyme to concanavalin A sepharose. Sepharose 225-234 tyrosinase Homo sapiens 156-166 7698335-1 1995 DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. Sepharose 113-122 elastase, neutrophil expressed Homo sapiens 10-29 7639849-9 1995 For FPLC using agarose resins, a low ionic strength, isotonic buffer system resulted in near total recoveries of CETP, and demonstrated a peak for CETP mass centered at molecular masses of 150 to 180 kilodaltons, larger than that for free monomeric CETP. Sepharose 15-22 cholesteryl ester transfer protein Homo sapiens 113-117 7639849-9 1995 For FPLC using agarose resins, a low ionic strength, isotonic buffer system resulted in near total recoveries of CETP, and demonstrated a peak for CETP mass centered at molecular masses of 150 to 180 kilodaltons, larger than that for free monomeric CETP. Sepharose 15-22 cholesteryl ester transfer protein Homo sapiens 147-151 7639849-9 1995 For FPLC using agarose resins, a low ionic strength, isotonic buffer system resulted in near total recoveries of CETP, and demonstrated a peak for CETP mass centered at molecular masses of 150 to 180 kilodaltons, larger than that for free monomeric CETP. Sepharose 15-22 cholesteryl ester transfer protein Homo sapiens 147-151 7639849-12 1995 Agarose gel electrophoresis showed plasma CETP, as well as purified recombinant CETP, to be prebeta migrating. Sepharose 0-7 cholesteryl ester transfer protein Homo sapiens 42-46 7696250-4 1995 The 68 kDa calmodulin-binding protein and the DNA polymerase alpha-primase complex cofractionate during Q-Sepharose chromatography to isolate the 21S enzyme complex, native and denatured DNA-cellulose to dissociate the 21S complex, and DEAE-Bio-Gel chromatography to isolate the multiprotein DNA polymerase alpha-primase complex. Sepharose 106-115 IQ motif containing J Homo sapiens 11-37 7696250-4 1995 The 68 kDa calmodulin-binding protein and the DNA polymerase alpha-primase complex cofractionate during Q-Sepharose chromatography to isolate the 21S enzyme complex, native and denatured DNA-cellulose to dissociate the 21S complex, and DEAE-Bio-Gel chromatography to isolate the multiprotein DNA polymerase alpha-primase complex. Sepharose 106-115 DNA polymerase alpha 1, catalytic subunit Homo sapiens 46-66 7696250-5 1995 The 68 kDa calmodulin-specific binding protein and DNA polymerase alpha also bind and coelute during affinity chromatography on calmodulin-agarose. Sepharose 139-146 calmodulin 1 Homo sapiens 11-21 7696250-5 1995 The 68 kDa calmodulin-specific binding protein and DNA polymerase alpha also bind and coelute during affinity chromatography on calmodulin-agarose. Sepharose 139-146 DNA polymerase alpha 1, catalytic subunit Homo sapiens 51-71 7696250-5 1995 The 68 kDa calmodulin-specific binding protein and DNA polymerase alpha also bind and coelute during affinity chromatography on calmodulin-agarose. Sepharose 139-146 calmodulin 1 Homo sapiens 128-138 7696250-6 1995 They also coprecipitate with C10-agarose-linked monoclonal antibody SJK 132-20 to human DNA polymerase alpha. Sepharose 33-40 DNA polymerase alpha 1, catalytic subunit Homo sapiens 88-108 7695632-3 1995 Elution on CaM-Sepharose of truncated ER molecules located the CaM binding domain of ER at the left edge of HBD. Sepharose 15-24 calmodulin 1 Rattus norvegicus 11-14 7695632-3 1995 Elution on CaM-Sepharose of truncated ER molecules located the CaM binding domain of ER at the left edge of HBD. Sepharose 15-24 estrogen receptor 1 Rattus norvegicus 38-40 7695632-3 1995 Elution on CaM-Sepharose of truncated ER molecules located the CaM binding domain of ER at the left edge of HBD. Sepharose 15-24 calmodulin 1 Rattus norvegicus 63-66 7695632-3 1995 Elution on CaM-Sepharose of truncated ER molecules located the CaM binding domain of ER at the left edge of HBD. Sepharose 15-24 estrogen receptor 1 Rattus norvegicus 85-87 7698335-1 1995 DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. Sepharose 113-122 secretory leukocyte peptidase inhibitor Homo sapiens 34-60 7698335-1 1995 DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. Sepharose 134-143 elastase, neutrophil expressed Homo sapiens 10-29 7698335-1 1995 DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. Sepharose 134-143 secretory leukocyte peptidase inhibitor Homo sapiens 34-60 7890776-10 1995 The major product of limited proteolysis of grp94 by chymotrypsin or papain is an N-terminal 85-kDa fragment that can bind to ATP-agarose but does not show autophosphorylation. Sepharose 130-137 heat shock protein 90 beta family member 1 Homo sapiens 44-49 7896441-8 1995 Using an affinity column of protein-A-Sepharose-bound IgG, purified from breast-cancer patients" sera, the BTAA was also recovered from HF1. Sepharose 38-47 complement factor H Homo sapiens 136-139 7892201-3 1995 NIH 3T3 cells expressing CBF beta-SMMHC acquired a transformed phenotype, as indicated by their ability to form foci, grow in soft agarose, and form tumors in nude mice. Sepharose 131-138 core binding factor beta Mus musculus 25-33 7892201-3 1995 NIH 3T3 cells expressing CBF beta-SMMHC acquired a transformed phenotype, as indicated by their ability to form foci, grow in soft agarose, and form tumors in nude mice. Sepharose 131-138 myosin, heavy polypeptide 11, smooth muscle Mus musculus 34-39 7890760-4 1995 The lysine binding of plasma Lp(a) and r-apo(a) in the culture supernatants of transfected HepG2 cells was analyzed by lysine-Sepharose affinity chromatography. Sepharose 126-135 lipoprotein(a) Homo sapiens 29-34 7890669-4 1995 The p53 kinase activity co-purifies with UV-activated c-Jun kinase activity on heparin-Sepharose and on a c-Jun (but not a v-Jun-) affinity column. Sepharose 87-96 transformation related protein 53, pseudogene Mus musculus 4-7 7890669-4 1995 The p53 kinase activity co-purifies with UV-activated c-Jun kinase activity on heparin-Sepharose and on a c-Jun (but not a v-Jun-) affinity column. Sepharose 87-96 jun proto-oncogene Mus musculus 54-59 7876259-6 1995 We used the soluble recombinant insulin receptor kinase domain, which phosphorylated hIRS-p30 with high affinity (Km = 11.9 +/- 0.8 microM), and affinity columns prepared by coupling hIRS-p30 to NHS-activated Sepharose for binding assays. Sepharose 209-218 insulin Homo sapiens 32-39 7876198-2 1995 Topoisomerase I-mediated relaxation assays, analyzed by one- and two-dimensional agarose gel electrophoresis, indicate that binding of recombinant human HMG-I/Y to closed circular DNA introduces positive supercoils at low protein to nucleotide molar ratios and negative supercoils at higher ratios. Sepharose 81-88 high mobility group AT-hook 1 Homo sapiens 153-160 7779399-4 1995 The Rapid-ABO technique involves a two-step process: (i) amplification of DNA samples using primer sets specific for the ABO alleles and (ii) electrophoresis and visualization of amplified ABO fragments on a 3% MetaPhor agarose gel. Sepharose 220-227 ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Homo sapiens 10-13 7893711-3 1995 eIF-4E plus associated proteins was purified from lysates by m7GTP-Sepharose chromatography. Sepharose 67-76 eukaryotic translation initiation factor 4E1 Drosophila melanogaster 0-6 7893711-9 1995 A major phosphoprotein which copurifies with eIF-4E on m7GTP-Sepharose shows decreased overall phosphorylation and decreased association with eIF-4E following heat shock. Sepharose 61-70 eukaryotic translation initiation factor 4E1 Drosophila melanogaster 45-51 7893711-9 1995 A major phosphoprotein which copurifies with eIF-4E on m7GTP-Sepharose shows decreased overall phosphorylation and decreased association with eIF-4E following heat shock. Sepharose 61-70 eukaryotic translation initiation factor 4E1 Drosophila melanogaster 142-148 7749849-6 1995 Glycation increased the negative charge of Lp(a) and LDL as monitored by electrophoresis and ion-exchange chromatography and also reduced the affinity of Lp(a) and LDL for heparin-Sepharose. Sepharose 180-189 lipoprotein(a) Homo sapiens 154-159 7780201-3 1995 Extract of human placenta with 2 M urea was applied to a Sepharose 4B column conjugated with galectin-1 purified from frog (Rana catesbeiana) eggs. Sepharose 57-66 galectin 1 Homo sapiens 93-103 11578463-1 1995 Point mutations of c-K-ras in ovarian cancer were detected by replacement of GGT of codon 12 by GAT, AGT, TGT and GTT, polymerase chain reaction, agarose gel electrophoresis and Southern blot hybridization with a digoxigenin detection system. Sepharose 146-153 KRAS proto-oncogene, GTPase Homo sapiens 19-26 7897148-7 1995 CONCLUSION: Immunologic IgE-mediated reactions to erythromycin do occur, and in vitro diagnosis of such reactions can be made by using Sepharose as a solid phase covalently linked to this drug. Sepharose 135-144 immunoglobulin heavy constant epsilon Homo sapiens 24-27 7730348-3 1995 Ligand cross-linking, ligand blotting, and uPA-Sepharose affinity chromatography revealed an increase in a cell surface uPA binding protein that corresponds to the uPAR on the basis of its affinity for uPA, M(r) of 50,000-55,000, and phosphatidylinositol-specific phospholipase C sensitivity. Sepharose 47-56 plasminogen activator, urokinase Homo sapiens 43-46 7730348-3 1995 Ligand cross-linking, ligand blotting, and uPA-Sepharose affinity chromatography revealed an increase in a cell surface uPA binding protein that corresponds to the uPAR on the basis of its affinity for uPA, M(r) of 50,000-55,000, and phosphatidylinositol-specific phospholipase C sensitivity. Sepharose 47-56 plasminogen activator, urokinase Homo sapiens 120-123 7730348-3 1995 Ligand cross-linking, ligand blotting, and uPA-Sepharose affinity chromatography revealed an increase in a cell surface uPA binding protein that corresponds to the uPAR on the basis of its affinity for uPA, M(r) of 50,000-55,000, and phosphatidylinositol-specific phospholipase C sensitivity. Sepharose 47-56 plasminogen activator, urokinase receptor Homo sapiens 164-168 7730348-3 1995 Ligand cross-linking, ligand blotting, and uPA-Sepharose affinity chromatography revealed an increase in a cell surface uPA binding protein that corresponds to the uPAR on the basis of its affinity for uPA, M(r) of 50,000-55,000, and phosphatidylinositol-specific phospholipase C sensitivity. Sepharose 47-56 plasminogen activator, urokinase Homo sapiens 120-123 7766891-7 1995 Escherichia coli containing the plasmid expressed a functional AAT isozyme which comigrated with the soybean AAT4 isozyme during agarose gel electrophoresis. Sepharose 129-136 aspartate aminotransferase glyoxysomal isozyme AAT1 precursor Glycine max 63-66 7861162-6 1995 It was shown that the 85-kDa protein bound specifically to an affinity column of Sepharose-beta II-(422-434) tubulin peptide, which contains the sequence of the MAP binding domain on beta II-tubulin. Sepharose 81-90 beta-Tubulin at 85D Drosophila melanogaster 109-116 7861162-6 1995 It was shown that the 85-kDa protein bound specifically to an affinity column of Sepharose-beta II-(422-434) tubulin peptide, which contains the sequence of the MAP binding domain on beta II-tubulin. Sepharose 81-90 beta-Tubulin at 85D Drosophila melanogaster 191-198 7876143-5 1995 The IFN gamma R-IgG3 fusion protein was secreted into the culture medium as a 175-kDa glycoprotein and was purified over Protein G-Sepharose, DEAE-Sepharose, and size exclusion chromatography. Sepharose 131-140 interferon gamma Cricetulus griseus 4-13 7876143-5 1995 The IFN gamma R-IgG3 fusion protein was secreted into the culture medium as a 175-kDa glycoprotein and was purified over Protein G-Sepharose, DEAE-Sepharose, and size exclusion chromatography. Sepharose 131-140 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 16-20 21597717-6 1995 The transient induction of p21/WAF1/CIP1 in early G1 was correlated with a transient decrease in p9Ckshs1-agarose precipitable histone H1 kinase activity, as determined by in vitro kinase assays. Sepharose 106-113 cyclin dependent kinase inhibitor 1A Homo sapiens 27-30 7876565-5 1995 First, the IgE-enriched DEAE-cellulose chromatography fraction was incubated in a batch mode with two alpha hIgE-Fc MAbs (HP6029, HP6061) coupled to CNBr-Sepharose, CL-4B. Sepharose 154-163 immunoglobulin heavy constant epsilon Homo sapiens 11-14 21597717-6 1995 The transient induction of p21/WAF1/CIP1 in early G1 was correlated with a transient decrease in p9Ckshs1-agarose precipitable histone H1 kinase activity, as determined by in vitro kinase assays. Sepharose 106-113 cyclin dependent kinase inhibitor 1A Homo sapiens 31-35 21597717-6 1995 The transient induction of p21/WAF1/CIP1 in early G1 was correlated with a transient decrease in p9Ckshs1-agarose precipitable histone H1 kinase activity, as determined by in vitro kinase assays. Sepharose 106-113 cyclin dependent kinase inhibitor 1A Homo sapiens 36-40 7532004-7 1995 To remove the sugar moiety, CHIP28 was enzymatically deglycosylated by PNGase F and purified by Q-Sepharose anion-exchange and Erythrina cristagalli lectin chromatography. Sepharose 98-107 aquaporin 1 (Colton blood group) Homo sapiens 28-34 7532004-3 1995 A new purification procedure based on phenylboronic acid-agarose (PBA) affinity chromatography was developed to isolate CHIP28. Sepharose 57-64 aquaporin 1 (Colton blood group) Homo sapiens 120-126 7852411-1 1995 Tissue plasminogen activator (tPA) was fractionated using lysine-Sepharose affinity chromatography. Sepharose 65-74 plasminogen activator, tissue type Homo sapiens 0-34 7531705-4 1995 First, native tenascin-C was bound to heparin-agarose and digested with Pronase. Sepharose 46-53 tenascin C Gallus gallus 14-24 7532400-9 1995 This enzyme could be partially purified from epimastigote plasma-membrane vesicles using calmodulin-agarose affinity chromatography. Sepharose 100-107 calmodulin Bos taurus 89-99 7864806-1 1995 Phospholipase A2 (PLA2) activity was purified 12,544-fold with a 13% yield from the plasma of patients diagnosed of septic shock by the sequential use of heparin-agarose affinity chromatography, gel filtration, and reverse-phase f.p.l.c. Sepharose 162-169 phospholipase A2 group IB Homo sapiens 0-16 7864806-1 1995 Phospholipase A2 (PLA2) activity was purified 12,544-fold with a 13% yield from the plasma of patients diagnosed of septic shock by the sequential use of heparin-agarose affinity chromatography, gel filtration, and reverse-phase f.p.l.c. Sepharose 162-169 phospholipase A2 group IB Homo sapiens 18-22 7862663-4 1995 Nuclear extracts depleted of galectin-3 by affinity adsorption on a lactose-agarose column were deficient in splicing activity. Sepharose 76-83 galectin 3 Homo sapiens 29-39 7852353-6 1995 Recombinant thrombospondin-4 has been purified from the culture supernatant by heparin-Sepharose and anti-thrombospondin-4 antibody-Affi-gel affinity chromatography. Sepharose 87-96 thrombospondin 4 Homo sapiens 12-28 7757233-0 1995 Intrinsic factor covalently bound to Sepharose as affinity medium for the purification of a soluble intrinsic factor receptor from human urine. Sepharose 37-46 cobalamin binding intrinsic factor Homo sapiens 0-16 7757233-0 1995 Intrinsic factor covalently bound to Sepharose as affinity medium for the purification of a soluble intrinsic factor receptor from human urine. Sepharose 37-46 cobalamin binding intrinsic factor Homo sapiens 100-116 7836475-2 1995 The binding of clathrin-coated vesicles, clathrin triskelions, and free clathrin light chains to calmodulin-Sepharose was compared. Sepharose 108-117 calmodulin Bos taurus 97-107 7836475-3 1995 When isolated from bovine brain, all three components bound to calmodulin-Sepharose in the presence of calcium and could be eluted by its removal. Sepharose 74-83 calmodulin Bos taurus 63-73 7836475-7 1995 Recombinant mutants of LCa, with deletions spanning the entire sequence, were tested for binding to calmodulin-Sepharose. Sepharose 111-120 clathrin light chain A Bos taurus 23-26 7836475-7 1995 Recombinant mutants of LCa, with deletions spanning the entire sequence, were tested for binding to calmodulin-Sepharose. Sepharose 111-120 calmodulin Bos taurus 100-110 7848263-2 1995 Cultures treated for 8 h with methional in the presence of IL3 show extensive DNA double-strand breaks on flow cytometric analysis, increases in DNA fragmentation as measured by the amount of non-sedimentable DNA present in the 30,000 g supernatant of cell lysates and the typical laddering pattern of multiples of 180 bp seen upon agarose gel electrophoresis. Sepharose 332-339 interleukin 3 Mus musculus 59-62 7848290-6 1995 CL-43 was purified by affinity chromatography on mannan-Sepharose. Sepharose 56-65 collectin-43 Bos taurus 0-5 7599706-5 1995 DNA bands obtained for inhibin alpha, beta A and ACTR 2 by agarose gel-electrophoresis were 367, 285, and 389 bp, respectively. Sepharose 59-66 actin related protein 2 Homo sapiens 49-55 7875375-2 1995 This 30-kDa protease, which we have termed ovochymase, was isolated from the exudate of activated eggs using a soy bean trypsin inhibitor-agarose affinity column. Sepharose 138-145 ovochymase 1 L homeolog Xenopus laevis 43-53 7538587-2 1995 When compared longterm in 60 experiments under standardized conditions, articular chondrocytes cultured in agarose exhibit variability in proteoglycan synthesis, and its suppression by interleukin 1 (IL-1), but a high reproducibility in the modulation of these effects by antirheumatic drugs. Sepharose 107-114 interleukin 1 alpha Homo sapiens 185-204 7823935-8 1995 The finding that the A2 and C proteins can bind to a calmodulin-Sepharose column in a Ca(2+)-dependent manner suggests that this association could prevent the phosphorylation of the proteins by casein kinase 2. Sepharose 64-73 calmodulin 1 Rattus norvegicus 53-63 8574192-2 1995 The competitor RNA harbors a 32-base deletion compared with wild-type EGFR mRNA and generates a PCR product that is easily distinguished from the wild-type PCR product by agarose gel electrophoresis. Sepharose 171-178 epidermal growth factor receptor Homo sapiens 70-74 7843421-4 1995 To purify leukotriene A4 hydrolase from human epidermis a new non-specific affinity chromatography column, with the leukotriene A4 hydrolase inhibitor bestatin coupled to AH-Sepharose, was introduced. Sepharose 174-183 leukotriene A4 hydrolase Homo sapiens 10-34 7851377-9 1995 A subclass of LpA-I with pre-beta migration on agarose electrophoresis was increased in CETP-D subjects. Sepharose 47-54 cholesteryl ester transfer protein Homo sapiens 88-92 7822313-8 1995 Using GSH-Sepharose to selectively bind GST constructs, tightly bound kinase was shown to rapidly transfer in a highly preferential way from intact E2 core to GST constructs containing the E2L2 domain rather than to ones containing only the E2L1 domain. Sepharose 10-19 glutathione S-transferase kappa 1 Homo sapiens 40-43 7822313-8 1995 Using GSH-Sepharose to selectively bind GST constructs, tightly bound kinase was shown to rapidly transfer in a highly preferential way from intact E2 core to GST constructs containing the E2L2 domain rather than to ones containing only the E2L1 domain. Sepharose 10-19 glutathione S-transferase kappa 1 Homo sapiens 159-162 7822313-9 1995 GST-E2L2-kinase complexes could be eluted from GSH-Sepharose with glutathione. Sepharose 51-60 glutathione S-transferase kappa 1 Homo sapiens 0-3 7811743-3 1995 Nearly all the LPL in post-heparin plasma of controls bound to heparin-Sepharose and this LPL bound was mainly eluted with 1.5 M NaCl in parallel with the activity. Sepharose 71-80 lipoprotein lipase Homo sapiens 15-18 8540332-1 1995 An acid proteinase of Dirofilaria immitis worms was purified 437-fold by gel filtration on Sephadex G-75 followed by pepstatin-Agarose gel affinity chromatography. Sepharose 127-134 endogenous retrovirus group K member 25 Homo sapiens 8-18 7832187-6 1995 Analysis of the proposita"s plasma by crossed immunoelectrophoresis in the presence or absence of heparin and by affinity chromatography on heparin-Sepharose revealed that the proposita"s AT III had apparently normal affinity for heparin. Sepharose 148-157 serpin family C member 1 Homo sapiens 188-194 7536836-2 1995 We found that at pH 7.2, both free PSA and PSA-ACT molecules are negatively charged and bind to the DEAE-Sepharose column. Sepharose 105-114 aminopeptidase puromycin sensitive Homo sapiens 35-38 7536836-2 1995 We found that at pH 7.2, both free PSA and PSA-ACT molecules are negatively charged and bind to the DEAE-Sepharose column. Sepharose 105-114 aminopeptidase puromycin sensitive Homo sapiens 43-46 7536836-4 1995 Both free PSA and PSA-ACT molecules were also found to be retained by the Con A Sepharose column because of the carbohydrate moiety of the PSA molecule. Sepharose 80-89 aminopeptidase puromycin sensitive Homo sapiens 10-13 7536836-4 1995 Both free PSA and PSA-ACT molecules were also found to be retained by the Con A Sepharose column because of the carbohydrate moiety of the PSA molecule. Sepharose 80-89 aminopeptidase puromycin sensitive Homo sapiens 18-21 7536836-4 1995 Both free PSA and PSA-ACT molecules were also found to be retained by the Con A Sepharose column because of the carbohydrate moiety of the PSA molecule. Sepharose 80-89 aminopeptidase puromycin sensitive Homo sapiens 18-21 7798663-4 1995 After incubation of the PCR product with Bsu36I and a subsequent agarose gel electrophoresis, the presence of the TAG mutant is revealed by an altered position of the DNA band. Sepharose 65-72 long intergenic non-protein coding RNA 1194 Homo sapiens 114-117 7528268-3 1995 Neurogranin for the study was purified from perchloric acid-soluble calf brain proteins by a combination of calmodulin-Sepharose affinity chromatography and reverse-phase HPLC. Sepharose 119-128 neurogranin Bos taurus 0-11 8577286-4 1995 LPS inhibited spontaneous and TNF-alpha-induced human neutrophil apoptosis in vitro, as determined by 1) light and electron microscopy, 2) flow cytometry, and 3) agarose gel electrophoresis of DNA. Sepharose 162-169 tumor necrosis factor Homo sapiens 30-39 8524477-2 1995 It has already been shown that concentrations of all IgG isotypes (except IgG3) are efficiently decreased by immunoadsorption (IA) on protein A linked to sepharose beads. Sepharose 154-163 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 74-78 7537919-5 1995 When beta 2GPI dependent IgG-aPL were purified using agarose-immobilized cardiolipin, 4 out of 4 preparations were highly positive in anti-beta 2GPI antibody ELISA using PVC plates, while beta 2GPI was not fully recognized by aPL-IgG when adsorbed onto PST plates. Sepharose 53-60 apolipoprotein H Homo sapiens 5-14 7537919-5 1995 When beta 2GPI dependent IgG-aPL were purified using agarose-immobilized cardiolipin, 4 out of 4 preparations were highly positive in anti-beta 2GPI antibody ELISA using PVC plates, while beta 2GPI was not fully recognized by aPL-IgG when adsorbed onto PST plates. Sepharose 53-60 apolipoprotein H Homo sapiens 139-148 7537919-5 1995 When beta 2GPI dependent IgG-aPL were purified using agarose-immobilized cardiolipin, 4 out of 4 preparations were highly positive in anti-beta 2GPI antibody ELISA using PVC plates, while beta 2GPI was not fully recognized by aPL-IgG when adsorbed onto PST plates. Sepharose 53-60 apolipoprotein H Homo sapiens 139-148 7818503-7 1994 Protamine was able to almost quantitatively release both lipoprotein lipase and hepatic lipase from columns of heparin-agarose. Sepharose 119-126 lipoprotein lipase Rattus norvegicus 57-75 7818503-7 1994 Protamine was able to almost quantitatively release both lipoprotein lipase and hepatic lipase from columns of heparin-agarose. Sepharose 119-126 lipase C, hepatic type Rattus norvegicus 80-94 7527659-5 1994 Direct evidence that these diSLex-containing structures are, indeed, high-affinity ligands for E-selectin came from the use of recombinant soluble E-selectin-agarose affinity chromatography. Sepharose 158-165 selectin E Homo sapiens 95-105 7527659-5 1994 Direct evidence that these diSLex-containing structures are, indeed, high-affinity ligands for E-selectin came from the use of recombinant soluble E-selectin-agarose affinity chromatography. Sepharose 158-165 selectin E Homo sapiens 147-157 7601270-3 1994 Pigeon fibrinogen, isolated from plasma by precipitation with PEG-1000 and purified over Sepharose 4B, was used to study receptor-ligand interaction. Sepharose 89-98 fibrinogen beta chain Homo sapiens 7-17 7779687-3 1994 Conversely, tubulin was recovered in association with p34cdc2 in p13-Sepharose pellets. Sepharose 69-78 cyclin-dependent kinase 1 L homeolog Xenopus laevis 54-61 7535784-6 1994 However, dBcAMP treatment increased the binding of alpha 1 beta 1 receptors to collagen type IV-Sepharose by a factor 2.3 +/- 0.6 (P < 0.02), while no alteration in the binding to collagen type I was detected. Sepharose 96-105 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 59-65 7751970-2 1994 Two 59Fe peaks (peaks 1 and 2) were separated on Sepharose 6B gel filtration from detergent solubilized 20,000 x g precipitate of upper intestinal mucosal homogenate after administration of 59Fe-labelled ferrous materials. Sepharose 49-58 pseudopodium-enriched atypical kinase 1 Rattus norvegicus 16-29 7603970-3 1995 The activated thrombin was isolated by a single Heparin-Sepharose affinity chromatography step. Sepharose 56-65 coagulation factor II, thrombin Bos taurus 14-22 7836779-5 1995 Treatment of cells to TNF lead to apoptosis as indicated by thymidine release, DNA laddering on agarose gels and morphological alterations. Sepharose 96-103 tumor necrosis factor Homo sapiens 22-25 7816827-5 1995 Kinetic properties of the DNA autoantibody Fab fragment were evaluated from the flow linear dichroism and agarose gel electrophoresis data and revealed a high affinity (Kobsm = 43 nM) and considerable catalytic efficiency (kappcat/Kobsm = 0.32 min-1.nM-1) of the reaction. Sepharose 106-113 FA complementation group B Homo sapiens 43-46 7832187-10 1995 Heparin affinity of purified abnormal AT III from the proposita"s plasma was demonstrated to be increased upon affinity chromatography using heparin-Sepharose, suggesting that the mutation (Arg393-His) per se could possibly increase the affinity of antithrombin III for heparin. Sepharose 149-158 serpin family C member 1 Homo sapiens 38-44 7832187-10 1995 Heparin affinity of purified abnormal AT III from the proposita"s plasma was demonstrated to be increased upon affinity chromatography using heparin-Sepharose, suggesting that the mutation (Arg393-His) per se could possibly increase the affinity of antithrombin III for heparin. Sepharose 149-158 serpin family C member 1 Homo sapiens 249-265 7710083-0 1995 Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose. Sepharose 131-138 titin Homo sapiens 41-50 7710083-0 1995 Detection of giant myofibrillar proteins connectin and nebulin by electrophoresis in 2% polyacrylamide slab gels strengthened with agarose. Sepharose 131-138 nebulin Homo sapiens 55-62 7779581-9 1995 Although native SOD has no affinity for heparin, the modified enzyme bound to a heparin-agarose column. Sepharose 88-95 superoxide dismutase 1 Homo sapiens 16-19 8789465-4 1995 Biotinyl-NPY was radiolabeled with Na125l by the chloramine-T method and bound to a streptavidin-agarose matrix. Sepharose 97-104 neuropeptide Y Homo sapiens 9-12 7702840-4 1995 Complexes containing both ATFa and either c-Jun or c-Fos were specifically retained on glutathione (GSH)-agarose beads as revealed by immunoblot analyses. Sepharose 105-112 activating transcription factor 7 Homo sapiens 26-30 7702840-4 1995 Complexes containing both ATFa and either c-Jun or c-Fos were specifically retained on glutathione (GSH)-agarose beads as revealed by immunoblot analyses. Sepharose 105-112 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 42-47 7702840-4 1995 Complexes containing both ATFa and either c-Jun or c-Fos were specifically retained on glutathione (GSH)-agarose beads as revealed by immunoblot analyses. Sepharose 105-112 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 51-56 7765987-2 1995 A second derivative of factor Xa in which a hexahistidine tail is fused to the C-terminus of the heavy chain (FXa-H6) also retains activity when immobilized, in this case on Ni(2+)-NTA agarose. Sepharose 185-192 coagulation factor X Homo sapiens 23-32 7765987-2 1995 A second derivative of factor Xa in which a hexahistidine tail is fused to the C-terminus of the heavy chain (FXa-H6) also retains activity when immobilized, in this case on Ni(2+)-NTA agarose. Sepharose 185-192 coagulation factor X Homo sapiens 110-113 7765987-3 1995 The stabilities and activities of of FXa-CBD and FXa-H6 immobilized on cellulose and Ni(2+)-NTA agarose, respectively, are similar. Sepharose 96-103 coagulation factor X Homo sapiens 37-40 7728656-5 1995 An affinity isolation technique based on biotinylated porcine transferrin plus streptavidin-agarose, followed by SDS-PAGE, allowed the isolation and identification of two potential porcine transferrin binding polypeptides (94 and 60 kDa) from total membranes derived from the type strain grown under iron-restricted conditions but only one (96 kDa) from strain E751. Sepharose 92-99 serotransferrin Bos taurus 189-200 7531118-4 1995 CD34+ cells were enriched from normal donors by apheresis and positive selection using an affinity column and plated in agarose with different combinations of cytokines. Sepharose 120-127 CD34 molecule Homo sapiens 0-4 8867780-12 1995 Furthermore, when GST:Cdc8p protein was expressed in yeast, the protein could bind to the glutathione-agarose, along with nucleoside diphosphate kinase, suggesting that there is an interaction between GST:Cdc8p and nucleoside diphosphate kinase in vivo. Sepharose 102-109 bifunctional thymidylate/uridylate kinase Saccharomyces cerevisiae S288C 22-27 8867780-12 1995 Furthermore, when GST:Cdc8p protein was expressed in yeast, the protein could bind to the glutathione-agarose, along with nucleoside diphosphate kinase, suggesting that there is an interaction between GST:Cdc8p and nucleoside diphosphate kinase in vivo. Sepharose 102-109 nucleoside diphosphate kinase Saccharomyces cerevisiae S288C 122-151 8867780-12 1995 Furthermore, when GST:Cdc8p protein was expressed in yeast, the protein could bind to the glutathione-agarose, along with nucleoside diphosphate kinase, suggesting that there is an interaction between GST:Cdc8p and nucleoside diphosphate kinase in vivo. Sepharose 102-109 bifunctional thymidylate/uridylate kinase Saccharomyces cerevisiae S288C 205-210 8867780-12 1995 Furthermore, when GST:Cdc8p protein was expressed in yeast, the protein could bind to the glutathione-agarose, along with nucleoside diphosphate kinase, suggesting that there is an interaction between GST:Cdc8p and nucleoside diphosphate kinase in vivo. Sepharose 102-109 nucleoside diphosphate kinase Saccharomyces cerevisiae S288C 215-244 7813084-0 1995 Apolipoprotein E2/E3/E4 genotyping with agarose gels. Sepharose 40-47 apolipoprotein E Homo sapiens 0-17 7858951-2 1995 A screen of tissues of a marine mollusc revealed a prominent intestine-specific cDNA encoding a pancreatic serine protease homolog, which was not detectable as a discrete poly(A)+ RNA species on formaldehyde agarose gels. Sepharose 208-215 coagulation factor II, thrombin Homo sapiens 107-122 7547035-6 1995 They were also retained on thrombin-Sepharose. Sepharose 36-45 coagulation factor II, thrombin Homo sapiens 27-35 7796532-3 1995 SRF binds WGA-agarose and apparently is O-glycosylated. Sepharose 14-21 serum response factor Homo sapiens 0-3 7775392-3 1995 After removal of guanidine HCl, rMME was purified with a Q-Sepharose column. Sepharose 59-68 membrane metallo-endopeptidase Rattus norvegicus 32-36 7775392-10 1995 Significant enzyme activity was present in rMME treated with EDTA followed by Q-Sepharose column chromatography. Sepharose 80-89 membrane metallo-endopeptidase Rattus norvegicus 43-47 7775400-4 1995 delta 54 beta-amylase was purified from the cells by consecutive alpha-cyclodextrin/Sepharose 6B column chromatography. Sepharose 84-93 1,4-alpha-D-glucan maltohydrolase Hordeum vulgare 9-21 7602422-6 1995 Both p120 and p185 are glycoproteins and were retained by the ConA Sepharose column. Sepharose 67-76 catenin delta 1 Homo sapiens 5-9 7602422-6 1995 Both p120 and p185 are glycoproteins and were retained by the ConA Sepharose column. Sepharose 67-76 eukaryotic translation initiation factor 3 subunit A Homo sapiens 14-18 7714662-2 1995 In this study, we purified the hEGF sample using Benzamidine-Sepharose 6B column chromatography as a critical step for purification and newly developed an EIA system with specificity for hEGF. Sepharose 61-70 epidermal growth factor Homo sapiens 31-35 7845020-4 1995 Agarose gel analysis of the PCR products was sufficient to discriminate between the absence of any of the four MLL rearrangements and the presence of at least one of them. Sepharose 0-7 lysine methyltransferase 2A Homo sapiens 111-114 7845020-6 1995 For this reason, automatic fluorescence-based DNA-fragment analysis was used to exactly define the MLL translocation partner genes if a positive result had been obtained by agarose gel analysis. Sepharose 173-180 lysine methyltransferase 2A Homo sapiens 99-102 7528891-1 1995 The capacity of two radiation-sensitive clones (SX9 and SX10) of the mouse mammary carcinoma cell line SR1 to rejoin radiation-induced DNA double-strand breaks (DSBs) was determined by pulsed-field agarose gel electrophoresis. Sepharose 198-205 spectrotype regulation Mus musculus 103-106 7747396-2 1995 A bovine TNF-alpha cDNA insert containing plasmid was used to produce a 237-bp cDNA competitive template which, when PCR-amplified with primers designed to amplify bovine TNF-alpha cDNA, resulted in a 147-bp product distinguishable by agarose gel electrophoresis from the 378-bp TNF-alpha RT-PCR-amplified cDNA product. Sepharose 235-242 tumor necrosis factor Bos taurus 9-18 7747396-2 1995 A bovine TNF-alpha cDNA insert containing plasmid was used to produce a 237-bp cDNA competitive template which, when PCR-amplified with primers designed to amplify bovine TNF-alpha cDNA, resulted in a 147-bp product distinguishable by agarose gel electrophoresis from the 378-bp TNF-alpha RT-PCR-amplified cDNA product. Sepharose 235-242 tumor necrosis factor Bos taurus 171-180 7747396-2 1995 A bovine TNF-alpha cDNA insert containing plasmid was used to produce a 237-bp cDNA competitive template which, when PCR-amplified with primers designed to amplify bovine TNF-alpha cDNA, resulted in a 147-bp product distinguishable by agarose gel electrophoresis from the 378-bp TNF-alpha RT-PCR-amplified cDNA product. Sepharose 235-242 tumor necrosis factor Bos taurus 171-180 7988718-2 1994 The MC-Sepharose bound protein phosphatase-1 (PP1) with high capacity and purified human PP1 gamma in one step from E. coli extracts. Sepharose 7-16 inorganic pyrophosphatase 1 Homo sapiens 23-44 7988718-2 1994 The MC-Sepharose bound protein phosphatase-1 (PP1) with high capacity and purified human PP1 gamma in one step from E. coli extracts. Sepharose 7-16 inorganic pyrophosphatase 1 Homo sapiens 46-49 7988718-2 1994 The MC-Sepharose bound protein phosphatase-1 (PP1) with high capacity and purified human PP1 gamma in one step from E. coli extracts. Sepharose 7-16 inorganic pyrophosphatase 1 Homo sapiens 89-92 7983056-2 1994 Here we report that GNT has been purified to apparent homogeneity from mouse kidney by means of preparation of a microsomal fraction, solubilization with Triton X-100, and sequential column chromatographies on CM-Sepharose, UDP-hexanolamine-Sepharose, and Gg4Ose-Aminocellulo-fine. Sepharose 213-222 glucosaminyl (N-acetyl) transferase family member 7 Mus musculus 20-23 7983056-2 1994 Here we report that GNT has been purified to apparent homogeneity from mouse kidney by means of preparation of a microsomal fraction, solubilization with Triton X-100, and sequential column chromatographies on CM-Sepharose, UDP-hexanolamine-Sepharose, and Gg4Ose-Aminocellulo-fine. Sepharose 241-250 glucosaminyl (N-acetyl) transferase family member 7 Mus musculus 20-23 7793356-1 1994 A 67 K outer membrane protein (OMP) isolated from phase I Coxiella burnetii QiYi strain was purified with monoclonal antibodies (MoAb) coupled to CNBr-Sepharose 4B. Sepharose 151-160 olfactory marker protein Mus musculus 31-34 7811387-2 1994 We have isolated a mitochondrial homolog (mtHSP70) from rat liver using ATP agarose affinity chromatography. Sepharose 76-83 heat shock protein family A (Hsp70) member 9 Rattus norvegicus 42-49 7890120-10 1994 Placental arylsulfatase A became bound to Lens culinaris agglutinin agarose, while no interaction with Ricinus communis or Griffonia simplicifolia agglutinin agarose was observed. Sepharose 68-75 arylsulfatase A Homo sapiens 10-25 7891388-5 1994 Heparin-binding fractions of FCS obtained by heparin-Sepharose chromatography synergized with TGF-beta 1 and beta 2 to produce a mitogenic response. Sepharose 53-62 hemoglobin, beta adult minor chain Mus musculus 109-115 7877136-2 1994 Detergent-solubilized insulin receptors from IM-9 cells immobilized on Sepharose beads by 960 antisera bound 2-3 times more 125I-insulin tracer (25-60 pM) than receptors immobilized with either 83-7 or CT-1. Sepharose 71-80 insulin Homo sapiens 22-29 7708061-9 1994 The results demonstrate that labeled estrogen receptor can bind to Pit-1 immobilized on glutathione agarose beads. Sepharose 100-107 POU class 1 homeobox 1 Rattus norvegicus 67-72 7947835-1 1994 The bifunctional enzyme 5-aminoimidazole-4-carboxamide ribotide (AICAR) transformylase-IMP cyclohydrolase has been purified 780-fold to apparent homogeneity from human CCRF-CEM leukemia cells, completed with chromatography on Affi-Gel Blue followed by AICAR-Sepharose 4B. Sepharose 258-267 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase Homo sapiens 65-70 7969171-7 1994 Agarose gel electrophoretic mobility shift showed that a preformed Zta-holo-TFIID-TFIIA complex was resistant to Sarkosyl and to Zta response element oligonucleotide challenge. Sepharose 0-7 general transcription factor IIA subunit 1 Homo sapiens 82-87 7961651-7 1994 In addition, bovine P450c17 binds reversibly to flavodoxin Sepharose in an ionic strength-dependent manner. Sepharose 59-68 steroid 17-alpha-hydroxylase/17,20 lyase Bos taurus 20-27 7961820-3 1994 The PCI fusion protein interfered in heparin-accelerated PCI-proteinase inhibition reactions, and it bound to heparin-Sepharose. Sepharose 118-127 endogenous retrovirus group K member 18 Homo sapiens 61-71 7961820-4 1994 Compared to the wild-type PCI fusion protein, deletion of the H helix from the fusion protein resulted in a reduction of both heparin-Sepharose binding and the ability to compete for heparin during PCI-proteinase inhibition reactions. Sepharose 134-143 endogenous retrovirus group K member 18 Homo sapiens 202-212 7525578-8 1994 Supporting evidence that intact full-length alpha v beta 8 could also bind to vitronectin-Sepharose was provided by performing affinity chromatography with the melanoma cell line MeWo, which normally expresses the intact beta 8 subunit. Sepharose 90-99 vitronectin Homo sapiens 78-89 7947757-1 1994 A Xenopus laevis casein kinase II-like activity copurified with X. laevis DNA topoisomerase I activity during chromatography on DEAE-cellulose, phosphocellulose, and hydroxylapatite, but the two activities were resolved by chromatography on DNA-agarose [Kaiserman, H. B., Ingebritsen, T. S., & Benbow, R. M. (1988) Biochemistry 27, 3216-3222]. Sepharose 245-252 DNA topoisomerase I, gene 1 L homeolog Xenopus laevis 74-93 7980644-7 1994 The carboxylesterase was purified from autopsy liver by gel filtration, chromatofocusing, ion-exchange, and hydrophobic interaction chromatography to purity by SDS-PAGE and agarose gel isoelectric focusing. Sepharose 173-180 carboxylesterase 1 Homo sapiens 4-20 7957681-3 1994 A recombinant human wild type p53 fused with glutathione S-transferase was immobilized on glutathione-agarose as a ligand for affinity column. Sepharose 102-109 tumor protein p53 Homo sapiens 30-33 7957681-3 1994 A recombinant human wild type p53 fused with glutathione S-transferase was immobilized on glutathione-agarose as a ligand for affinity column. Sepharose 102-109 glutathione S-transferase kappa 1 Homo sapiens 45-70 7863825-5 1994 Partial purification of cartilage-derived bFGF was performed on crude extracts of cartilage using heparin-Sepharose affinity chromatography. Sepharose 106-115 fibroblast growth factor 2 Homo sapiens 42-46 7863825-6 1994 The presence of bFGF in the heparin-Sepharose column fractions was confirmed by immunoblotting and radioimmunoassay. Sepharose 36-45 fibroblast growth factor 2 Homo sapiens 16-20 7530727-4 1994 Hsp56 bound to an antibody-Sepharose column binds 3H-FK506 with an affinity of 19.4 +/- 4.6 nM, as compared to 23.2 +/- 6.8 nM for soluble hsp56. Sepharose 27-36 FKBP prolyl isomerase 4 Homo sapiens 0-5 7929434-2 1994 The D-glucuronyl C-5 epimerase involved in the biosynthesis of heparin/heparan sulfate was purified from the high speed supernatant fraction of a homogenate of bovine liver by chromatography on immobilized O-desulfated heparin, red Sepharose, phenyl Sepharose, and concanavalin A-Sepharose. Sepharose 232-241 glucuronic acid epimerase Bos taurus 4-30 7831205-4 1994 At the same time, the ceruloplasmin-free protein preparation eluted in a first peak, following further Q-Sepharose and Con A-Sepharose chromatography, leads to purified bovine serum amine oxidase (BSAO) with an improved yield. Sepharose 105-114 ceruloplasmin and hephaestin like 1 Bos taurus 22-35 7831205-4 1994 At the same time, the ceruloplasmin-free protein preparation eluted in a first peak, following further Q-Sepharose and Con A-Sepharose chromatography, leads to purified bovine serum amine oxidase (BSAO) with an improved yield. Sepharose 105-114 primary amine oxidase, liver isozyme Bos taurus 176-195 7831209-2 1994 Cibacron Blue F3GA, the chromophore of Blue Sepharose, inhibited the activity of fumarate reductase. Sepharose 44-53 fumarate reductase Saccharomyces cerevisiae S288C 81-99 7929392-5 1994 Both APLP1 and APLP2 bound heparin-Sepharose and had NaCl elution profiles similar to that of APP. Sepharose 35-44 amyloid beta precursor like protein 1 Homo sapiens 5-10 7929392-5 1994 Both APLP1 and APLP2 bound heparin-Sepharose and had NaCl elution profiles similar to that of APP. Sepharose 35-44 amyloid beta precursor like protein 2 Homo sapiens 15-20 7929392-6 1994 As previously reported for APP, zinc increased the recovery of APLP1 and APLP2 upon heparin-Sepharose chromatography. Sepharose 92-101 amyloid beta precursor like protein 1 Homo sapiens 63-68 7929392-6 1994 As previously reported for APP, zinc increased the recovery of APLP1 and APLP2 upon heparin-Sepharose chromatography. Sepharose 92-101 amyloid beta precursor like protein 2 Homo sapiens 73-78 7930536-0 1994 A floating medium for transfer of PEG sections with agarose blotting technique. Sepharose 52-59 progestagen associated endometrial protein Homo sapiens 34-37 7929434-2 1994 The D-glucuronyl C-5 epimerase involved in the biosynthesis of heparin/heparan sulfate was purified from the high speed supernatant fraction of a homogenate of bovine liver by chromatography on immobilized O-desulfated heparin, red Sepharose, phenyl Sepharose, and concanavalin A-Sepharose. Sepharose 250-259 glucuronic acid epimerase Bos taurus 4-30 7929392-7 1994 APP, APLP1, and APLP2 all bind zinc-chelating Sepharose, indicating that the zinc binding motif may be functionally conserved in these proteins. Sepharose 46-55 amyloid beta precursor like protein 1 Homo sapiens 5-10 7929392-7 1994 APP, APLP1, and APLP2 all bind zinc-chelating Sepharose, indicating that the zinc binding motif may be functionally conserved in these proteins. Sepharose 46-55 amyloid beta precursor like protein 2 Homo sapiens 16-21 7700563-3 1994 Fibrin-agarose zymographies show that tissue-type PA (tPA) is the main PA associated with these structures. Sepharose 7-14 plasminogen activator, tissue type Rattus norvegicus 38-52 7524207-4 1994 The importance of the Gal alpha(1,3)Gal epitope was supported by (1) sugar inhibition studies; (2) complete absorption of cytotoxic antibodies by melibiose-sepharose columns; and (3) the ability of normal human serum to lyse COS cells after transfection with a cDNA clone encoding alpha(1,3)galactosyl transferase. Sepharose 156-165 galanin and GMAP prepropeptide Sus scrofa 22-25 7524207-4 1994 The importance of the Gal alpha(1,3)Gal epitope was supported by (1) sugar inhibition studies; (2) complete absorption of cytotoxic antibodies by melibiose-sepharose columns; and (3) the ability of normal human serum to lyse COS cells after transfection with a cDNA clone encoding alpha(1,3)galactosyl transferase. Sepharose 156-165 galanin and GMAP prepropeptide Sus scrofa 36-39 7700563-3 1994 Fibrin-agarose zymographies show that tissue-type PA (tPA) is the main PA associated with these structures. Sepharose 7-14 plasminogen activator, tissue type Rattus norvegicus 54-57 7947966-1 1994 A 120 kDa plasma protein, which is susceptible to plasma kallikrein, was purified from human plasma by polyethylene glycol fractionation followed by ion exchange chromatography using Q-Sepharose, S-Sepharose, and hydroxyapatite and gel filtration on Sephacryl S-200. Sepharose 185-194 kallikrein B1 Homo sapiens 50-67 7929379-9 1994 When SAPKs were immunodepleted from kidney extracts prior to incubation of the extracts with agarose-coupled GST-c-Jun(1-135), it was found that SAPKs accounted for the majority of the amino-terminal c-Jun kinase activity of kidney at 5 min following reperfusion. Sepharose 93-100 Jun proto-oncogene, AP-1 transcription factor subunit Canis lupus familiaris 113-118 7523391-6 1994 Degradation of recombinant human (rh) IGFBP-3 by conditioned media was blocked (> 80% inhibition) by tissue inhibitor of metallo-proteinases-1, a specific inhibitor of all MMPs, while removal of MMPs -1, -2, and -3 from conditioned medium by sequential immunoaffinity and gelatin-Sepharose chromatography resulted in the complete loss of IGFBP-3-degrading proteinase activity. Sepharose 283-292 insulin like growth factor binding protein 3 Homo sapiens 38-45 7937896-4 1994 Affinity chromatography was used to bind glutaredoxin on a glutathione-containing thiol-Sepharose column. Sepharose 88-97 glutaredoxin Homo sapiens 41-53 7850992-9 1994 In consequence, since it is reliable and relatively inexpensive, this detection test on modified agarose gel appears very useful for revealing the presence of abnormally high values of Lp(a) in populations. Sepharose 97-104 lipoprotein(a) Homo sapiens 185-190 7859819-7 1994 Furthermore, this labeled polypeptide could be specifically eluted from columns of Sepharose conjugated with antibody against rabbit plasma transferrin. Sepharose 83-92 serotransferrin Oryctolagus cuniculus 140-151 7945230-11 1994 The BSP proteins were adsorbed on PLA2-agarose and could be affinity cross-linked to the enzyme, indicating a direct interaction of enzyme with the inhibitor. Sepharose 39-46 LOC104974671 Bos taurus 34-38 7929600-5 1994 When the same 24-kD bFGF cDNA was expressed in E. coli, the recombinant protein was purified to homogeneity by heparin-Sepharose and ion-exchange chromatography. Sepharose 119-128 fibroblast growth factor 2 Homo sapiens 20-24 7535137-4 1994 Furthermore, L-929 PNGase was revealed to bind to the glycan moiety of yeast mannan by using mannan-conjugated Sepharose 4B gel as a ligand, suggesting that L-929 PNGase could serve not only as an enzyme but also as a carbohydrate recognition protein in vivo. Sepharose 111-123 N-glycanase 1 Homo sapiens 19-25 7535137-4 1994 Furthermore, L-929 PNGase was revealed to bind to the glycan moiety of yeast mannan by using mannan-conjugated Sepharose 4B gel as a ligand, suggesting that L-929 PNGase could serve not only as an enzyme but also as a carbohydrate recognition protein in vivo. Sepharose 111-123 N-glycanase 1 Homo sapiens 163-169 7881184-5 1994 The recombinant alpha-mannosidase was present in the medium at a level of approximately 1 mg/l and was purified in a single step by chromatography on S-Sepharose. Sepharose 152-161 alpha-mannosidase Saccharomyces cerevisiae S288C 16-33 7851631-3 1994 PHGPx was isolated from human liver using ammonium sulphate fractionation, affinity chromatography on bromosulphophthalein-glutathione-agarose, gel filtration on Sephadex G-50, anion exchange chromatography on Mono Q resin and high resolution gel filtration on Superdex 75. Sepharose 135-142 glutathione peroxidase 4 Homo sapiens 0-5 7654517-5 1994 The elution profiles displayed by RC3 and sequence variants during purification on CaM-Sepharose columns suggest that two different affinity forms of the RC3.CaM complex coexist when Ca2+ is absent and that GAP-43.CaM interactions are far more sensitive to salt than those that occur between recombinant RC3 and CaM. Sepharose 87-96 calmodulin 1 Homo sapiens 83-86 8089481-4 1994 In contrast, stimulation with SPA containing S. aureus or SPA-Sepharose resulted in biased expression of VH3 containing IgM. Sepharose 62-71 surfactant protein A1 Homo sapiens 58-61 8089481-4 1994 In contrast, stimulation with SPA containing S. aureus or SPA-Sepharose resulted in biased expression of VH3 containing IgM. Sepharose 62-71 immunoglobulin heavy variable 3-75 (pseudogene) Homo sapiens 105-108 7654517-5 1994 The elution profiles displayed by RC3 and sequence variants during purification on CaM-Sepharose columns suggest that two different affinity forms of the RC3.CaM complex coexist when Ca2+ is absent and that GAP-43.CaM interactions are far more sensitive to salt than those that occur between recombinant RC3 and CaM. Sepharose 87-96 neurogranin Homo sapiens 154-157 7654517-5 1994 The elution profiles displayed by RC3 and sequence variants during purification on CaM-Sepharose columns suggest that two different affinity forms of the RC3.CaM complex coexist when Ca2+ is absent and that GAP-43.CaM interactions are far more sensitive to salt than those that occur between recombinant RC3 and CaM. Sepharose 87-96 calmodulin 1 Homo sapiens 158-161 7654517-5 1994 The elution profiles displayed by RC3 and sequence variants during purification on CaM-Sepharose columns suggest that two different affinity forms of the RC3.CaM complex coexist when Ca2+ is absent and that GAP-43.CaM interactions are far more sensitive to salt than those that occur between recombinant RC3 and CaM. Sepharose 87-96 growth associated protein 43 Homo sapiens 207-213 7654517-5 1994 The elution profiles displayed by RC3 and sequence variants during purification on CaM-Sepharose columns suggest that two different affinity forms of the RC3.CaM complex coexist when Ca2+ is absent and that GAP-43.CaM interactions are far more sensitive to salt than those that occur between recombinant RC3 and CaM. Sepharose 87-96 calmodulin 1 Homo sapiens 158-161 7930945-3 1994 My43 immunoblotted Fc alpha R that had been affinity purified from PMN membranes and immunoprecipitated a 50-70 kDa protein from radiolabeled PMN membranes, apparently identical to that purified on IgA-Sepharose. Sepharose 202-211 Fc alpha receptor Homo sapiens 19-29 7654517-5 1994 The elution profiles displayed by RC3 and sequence variants during purification on CaM-Sepharose columns suggest that two different affinity forms of the RC3.CaM complex coexist when Ca2+ is absent and that GAP-43.CaM interactions are far more sensitive to salt than those that occur between recombinant RC3 and CaM. Sepharose 87-96 neurogranin Homo sapiens 154-157 7654517-5 1994 The elution profiles displayed by RC3 and sequence variants during purification on CaM-Sepharose columns suggest that two different affinity forms of the RC3.CaM complex coexist when Ca2+ is absent and that GAP-43.CaM interactions are far more sensitive to salt than those that occur between recombinant RC3 and CaM. Sepharose 87-96 calmodulin 1 Homo sapiens 158-161 7945307-2 1994 We find that suramin strongly inhibits the catalytic activity of purified p34cdc2 kinase (IC50 approximately 4 microM), whereas it only weakly inhibits the p13-agarose precipitated kinase activity from nuclear and cytoplasmic extracts of the asynchronous H69 human small cell lung cancer cells. Sepharose 160-167 H3 histone pseudogene 6 Homo sapiens 156-159 7827508-7 1994 In addition, we show here that the bacterially expressed Fab4-BCCP* complexes, adsorbed to streptavidin-agarose beads, can be used for the one-step purification of recombinant TNF by immunoaffinity chromatography. Sepharose 104-111 tumor necrosis factor Homo sapiens 176-179 7945307-3 1994 We also find that the tyrosine phosphorylation of p34cdc2 kinase in the nuclear extract is increased about twice when the extracts are preincubated with 50 microM of suramin prior to the p13-agarose precipitation. Sepharose 191-198 cyclin dependent kinase 1 Homo sapiens 50-57 7945307-3 1994 We also find that the tyrosine phosphorylation of p34cdc2 kinase in the nuclear extract is increased about twice when the extracts are preincubated with 50 microM of suramin prior to the p13-agarose precipitation. Sepharose 191-198 H3 histone pseudogene 6 Homo sapiens 187-190 8083232-7 1994 The VLDL receptor present in detergent extracts from transfected cells bound to RAP-Sepharose, and a KD of 0.7 nM for the interaction between RAP and the purified VLDL receptor was determined using enzyme-linked immunosorbent assay. Sepharose 84-93 very low-density lipoprotein receptor Cricetulus griseus 4-17 8089141-2 1994 A protein that binds to the MRE sequence in a zinc-dependent manner (zinc regulatory factor; ZRF) was purified 16,000-fold from HeLa cell nuclear extracts by means of the avidin-biotin method, in which a complex formed between ZRF and a biotinylated probe containing MRE was trapped by streptavidin-agarose beads, and ZRF was recovered by salt extraction. Sepharose 299-306 metal regulatory transcription factor 1 Homo sapiens 93-96 8089141-2 1994 A protein that binds to the MRE sequence in a zinc-dependent manner (zinc regulatory factor; ZRF) was purified 16,000-fold from HeLa cell nuclear extracts by means of the avidin-biotin method, in which a complex formed between ZRF and a biotinylated probe containing MRE was trapped by streptavidin-agarose beads, and ZRF was recovered by salt extraction. Sepharose 299-306 metal regulatory transcription factor 1 Homo sapiens 227-230 8089141-2 1994 A protein that binds to the MRE sequence in a zinc-dependent manner (zinc regulatory factor; ZRF) was purified 16,000-fold from HeLa cell nuclear extracts by means of the avidin-biotin method, in which a complex formed between ZRF and a biotinylated probe containing MRE was trapped by streptavidin-agarose beads, and ZRF was recovered by salt extraction. Sepharose 299-306 metal regulatory transcription factor 1 Homo sapiens 227-230 8083206-2 1994 To accomplish this goal, a subset of cytosolic CaM-binding proteins (CaMBPs) was partially purified by a combination of DE52 and CaM-Sepharose affinity chromatography. Sepharose 133-142 calmodulin Oryctolagus cuniculus 47-50 8083206-2 1994 To accomplish this goal, a subset of cytosolic CaM-binding proteins (CaMBPs) was partially purified by a combination of DE52 and CaM-Sepharose affinity chromatography. Sepharose 133-142 calmodulin Oryctolagus cuniculus 69-72 8086476-6 1994 Soluble HMG 1 depleted of its C-terminal domain interacted with tubulin immobilized on an agarose gel and with microtubules formed from purified tubulin. Sepharose 90-97 high mobility group box 1 Rattus norvegicus 8-13 7985993-1 1994 The distribution of plasma alpha 1B-glycoprotein (A1BG) was determined by a two-dimensional electrophoresis (agarose-polyacrylamide gel) followed by protein staining in a group of 1099 individuals from 11 populations of the Indian subcontinent. Sepharose 109-116 alpha-1-B glycoprotein Homo sapiens 27-48 7985993-1 1994 The distribution of plasma alpha 1B-glycoprotein (A1BG) was determined by a two-dimensional electrophoresis (agarose-polyacrylamide gel) followed by protein staining in a group of 1099 individuals from 11 populations of the Indian subcontinent. Sepharose 109-116 alpha-1-B glycoprotein Homo sapiens 50-54 8080270-4 1994 Qualitatively similar results were obtained (i.e., inhibition and ionic strength effect) when the GAPDH-catalyzed reaction was measured in the presence of Sepharose-immobilized tubulin. Sepharose 155-164 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 98-103 8080270-5 1994 The physical interaction between these two proteins, i.e., GAPDH and tubulin, was measured by the ability of one protein (immobilized on a Sepharose matrix) to copellet the other protein. Sepharose 139-148 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 59-64 7802604-11 1994 Moreover, the apoptosis of eosinophils induced by TGF-beta 1 was determined with the assay of DNA fragmentation on agarose gel electrophoresis. Sepharose 115-122 transforming growth factor beta 1 Homo sapiens 50-60 7988042-10 1994 EC-SOD in Group II showed two different profiles on heparin-Sepharose column chromatographies: one consisted mainly of EC-SOD C and the other consisted of EC-SOD A and C. It is probable that the high serum EC-SOD level in hemodialysis patients was due to two possible factors: the genetic transmitted factor and unknown pathophysiological factor(s). Sepharose 60-69 superoxide dismutase 3 Homo sapiens 0-6 8088431-6 1994 Here, by criteria of mRNA mobility on agarose gels, primer extension analysis, and differential Northern hybridization, we show that the promoter previously considered to be liver-specific is operative in SiaT-1 expression in the small intestine of suckling animals. Sepharose 38-45 ST6 beta-galactoside alpha-2,6-sialyltransferase 1 Rattus norvegicus 205-211 21559621-8 1994 p53 protein was studied by Western-blot and apoptosis in DNA agarose gels. Sepharose 61-68 transformation related protein 53, pseudogene Mus musculus 0-3 7530543-2 1994 A mouse anti-bombesin monoclonal antibody was immobilized to agarose beads and then the antigen, gastrin-releasing peptide (GRP), was allowed to bind. Sepharose 61-68 gastrin releasing peptide Homo sapiens 13-21 21559651-5 1994 Further evidence that these isoforms are cathepsin D came from studies in which the pepstatin-inhibitable protease activity and not the pepstatin-uninhibitable protease activity, bound to and was elutable from, an immunoaffinity resin made by coupling anticathepsin D polyclonal antibodies to agarose. Sepharose 293-300 cathepsin D Homo sapiens 41-52 7831282-1 1994 Rat glutathione transferase (GST) 3-3 binds to Ni(II)-iminodiacetic acid (IDA)-agarose, whereas other GSTs that are abundant in rat liver do not bind to this immobilized metal ion affinity chromatography (IMAC) adsorbent. Sepharose 79-86 glutathione S-transferase mu 1 Rattus norvegicus 0-37 7972519-5 1994 Polyclonal antibodies raised against the fusion project and purified by affinity chromatography on a GST-ASA1-Sepharose column cross-reacted with a 61.5-kD protein in a partially purified anthranilate synthase preparation from corn seedlings. Sepharose 110-119 glutathione S-transferase tau 5 Arabidopsis thaliana 101-104 7972519-5 1994 Polyclonal antibodies raised against the fusion project and purified by affinity chromatography on a GST-ASA1-Sepharose column cross-reacted with a 61.5-kD protein in a partially purified anthranilate synthase preparation from corn seedlings. Sepharose 110-119 anthranilate synthase alpha subunit 1 Arabidopsis thaliana 105-109 7822240-5 1994 Apolipoprotein (apo) E-free HDL2 from the patients, separated by heparin-Sepharose column chromatography, was rich in CE, poor in triglycerides (TG), and enlarged in size on 4-30% nondenaturing polyacrylamide gradient gel electrophoresis. Sepharose 73-82 apolipoprotein E Homo sapiens 0-22 7839663-3 1994 LDH C4 was isolated as follows: precipitation with ammonium sulfate, separation of LDH C4 from other LDH isoenzymes using immunosorbents, purification from contaminating proteins of oxamate-Sepharose. Sepharose 190-199 lactate dehydrogenase C Homo sapiens 0-3 7839663-5 1994 Elution of LDH C4 from oxamate-Sepharose by means of lithium lactate appeared inadequate therefore LDH C4 was eluted using 0.63 mM sodium pyruvate. Sepharose 31-40 lactate dehydrogenase C Homo sapiens 11-14 8078898-3 1994 The recombinant Hx proteins were purified by sequential chromatography on Con A-agarose and SP-Sepharose. Sepharose 80-87 hemopexin Homo sapiens 16-18 7915111-2 1994 Recent studies in this laboratory have shown that BSP is capable of nucleating the bone mineral hydroxyapatite in a steady-state agarose gel system. Sepharose 129-136 integrin binding sialoprotein Homo sapiens 50-53 8060336-1 1994 A 41,000 protein, p41, was highly purified from human promyelocytic HL-60 cells by chromatography on GTP-agarose columns. Sepharose 105-112 mitogen-activated protein kinase 1 Homo sapiens 18-21 8060353-6 1994 Our present data suggest a direct interaction of hsp90 with histones, showing that hsp90 is able to bind histone-agarose and enhances the binding of histones to DNA. Sepharose 113-120 heat shock protein 90 alpha family class A member 1 Rattus norvegicus 49-54 8060353-6 1994 Our present data suggest a direct interaction of hsp90 with histones, showing that hsp90 is able to bind histone-agarose and enhances the binding of histones to DNA. Sepharose 113-120 heat shock protein 90 alpha family class A member 1 Rattus norvegicus 83-88 8076651-1 1994 Carboxylester lipase obtained from pig pancreas is associated with fatty acid ethyl ester synthase as judged by their elution in the same fraction from a heparin-Sepharose column, coprecipitations by antibody against purified carboxylester lipase and identical profiles of inhibition by diisopropyl fluorophosphate. Sepharose 162-171 carboxyl ester lipase Sus scrofa 0-20 8049265-6 1994 Northern blot analysis of RNA prepared from the tRNA-Sepharose fraction showed the presence of 55SrRNA blot analysis of the tRNA-Sepharose fraction using an antibody against ribosomal protein L5, showed the presence of ribosomal protein L5 in this fraction. Sepharose 53-62 ribosomal protein L5 Rattus norvegicus 174-194 8049265-6 1994 Northern blot analysis of RNA prepared from the tRNA-Sepharose fraction showed the presence of 55SrRNA blot analysis of the tRNA-Sepharose fraction using an antibody against ribosomal protein L5, showed the presence of ribosomal protein L5 in this fraction. Sepharose 53-62 ribosomal protein L5 Rattus norvegicus 219-239 8049265-6 1994 Northern blot analysis of RNA prepared from the tRNA-Sepharose fraction showed the presence of 55SrRNA blot analysis of the tRNA-Sepharose fraction using an antibody against ribosomal protein L5, showed the presence of ribosomal protein L5 in this fraction. Sepharose 129-138 ribosomal protein L5 Rattus norvegicus 174-194 8049265-6 1994 Northern blot analysis of RNA prepared from the tRNA-Sepharose fraction showed the presence of 55SrRNA blot analysis of the tRNA-Sepharose fraction using an antibody against ribosomal protein L5, showed the presence of ribosomal protein L5 in this fraction. Sepharose 129-138 ribosomal protein L5 Rattus norvegicus 219-239 8052664-4 1994 Column chromatography, including modified affinity chromatography on lisinopril-Sepharose, yielded homogeneous ACE after only a 45-fold purification. Sepharose 80-89 angiotensin I converting enzyme Homo sapiens 111-114 8037455-1 1994 Ribonuclease inhibitor (RI) was purified about 1300-fold from human cerebrum (including a small portion of midbrain) by a combination of ammonium sulfate precipitation, ribonuclease A-Sepharose chromatography, and high-performance anion-exchange chromatography. Sepharose 184-193 ribonuclease/angiogenin inhibitor 1 Homo sapiens 0-27 7822240-5 1994 Apolipoprotein (apo) E-free HDL2 from the patients, separated by heparin-Sepharose column chromatography, was rich in CE, poor in triglycerides (TG), and enlarged in size on 4-30% nondenaturing polyacrylamide gradient gel electrophoresis. Sepharose 73-82 junctophilin 3 Homo sapiens 28-32 7808470-6 1994 Consistent with hydrophilicity of its primary sequence and the presence of a nucleotide binding site, p36 is a soluble molecule non-sedimentable at 105,000 x g and binds Blue Sepharose, elutable only with NADPH. Sepharose 175-184 annexin A2 Homo sapiens 102-105 7801727-3 1994 By affinity column on Sepharose 4 B coupled with 1 G 10 IgG, colonic carcinoma-associated antigen (CCA) was purified from SW 620 cell extracts and thoracic ascitic fluid of a patient with lung adenocarcinoma. Sepharose 22-31 fibrillin 2 Homo sapiens 61-103 8047075-5 1994 Moreover, soluble forms of the alpha/beta heterodimer will bind tightly to FL substituted sepharose, which demonstrates the retention of biological activity by the TCR after solubilization. Sepharose 90-99 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 164-167 7980949-3 1994 beta-glucuronidase was purified by ion exchange chromatography, saccharolactone-agarose affinity chromatography, and gel filtration. Sepharose 80-87 glucuronidase beta Homo sapiens 0-18 7950376-3 1994 Latent recombinant PAI-1 was purified by two chromatographic steps, cation exchange chromatography on CM-Sepharose and affinity chromatography on heparin-Sepharose. Sepharose 105-114 serpin family E member 1 Homo sapiens 19-24 7950376-3 1994 Latent recombinant PAI-1 was purified by two chromatographic steps, cation exchange chromatography on CM-Sepharose and affinity chromatography on heparin-Sepharose. Sepharose 154-163 serpin family E member 1 Homo sapiens 19-24 8021956-6 1994 To determine if there was heterozygosity of the Alu I and Apa I restriction site polymorphism in the H19 and IGF2 genes, respectively, DNA was isolated from cells of the peripheral blood of these patients, then subjected to polymerase chain reaction (PCR) amplification, restriction enzyme digestion, and electrophoresis in agarose gels. Sepharose 324-331 H19 imprinted maternally expressed transcript Homo sapiens 101-104 8033073-5 1994 We used alkaline treatment, affinity chromatography on Cm-papain Sepharose, followed by gel filtration and ion-exchange chromatography and anti-Stefin B-Sepharose 4B to isolate two major isoforms of Stefin B with pI values 5.9 and 6.5. Sepharose 153-162 cystatin-B Bos taurus 144-152 7974391-3 1994 We have developed an assay based on the use of heparin-Sepharose micro columns in order to compare small quantities of heparin fractions as well as different glycosaminoglycans on a weight basis for their TFPI binding. Sepharose 55-64 tissue factor pathway inhibitor Homo sapiens 205-209 8043002-10 1994 Essentially pure NTE was obtained after separation from two endogenous biotinylated polypeptides (120 and 70 kDa) in avidin-Sepharose eluates by preparative SDS/PAGE. Sepharose 124-133 patatin like phospholipase domain containing 6 Homo sapiens 17-20 7974391-4 1994 In this assay a glycosaminoglycan in solution compete with heparin-Sepharose for TFPI binding. Sepharose 67-76 tissue factor pathway inhibitor Homo sapiens 81-85 8027112-8 1994 In the agarose gels, chondrogenesis from the periosteum was observed at four and six weeks and was enhanced by the presence of one or ten nanograms of transforming growth factor-beta 1 per milliliter. Sepharose 7-14 LOW QUALITY PROTEIN: transforming growth factor beta-1 Oryctolagus cuniculus 151-184 8027112-9 1994 The combination of agarose with transforming growth factor-beta 1 most favored the formation of cartilage, which was maximum at six weeks in the presence of ten nanograms of transforming growth factor-beta 1 per milliliter. Sepharose 19-26 LOW QUALITY PROTEIN: transforming growth factor beta-1 Oryctolagus cuniculus 174-207 8040267-8 1994 Morphology and analysis of DNA after separation on agarose gels showed that IL-10 inhibits loss of cell volume, chromatin condensation, and DNA fragmentation, characteristics of death by apoptosis. Sepharose 51-58 interleukin 10 Homo sapiens 76-81 8040268-4 1994 The supernatant of thrombin-stimulated platelets contained an inhibitor of bFGF-induced mitogenesis; this activity coeluted with PF 4 upon gel filtration, heparin-agarose, and ion-exchange chromatography. Sepharose 163-170 coagulation factor II, thrombin Homo sapiens 19-27 8040268-4 1994 The supernatant of thrombin-stimulated platelets contained an inhibitor of bFGF-induced mitogenesis; this activity coeluted with PF 4 upon gel filtration, heparin-agarose, and ion-exchange chromatography. Sepharose 163-170 fibroblast growth factor 2 Homo sapiens 75-79 7911495-4 1994 Covalent coupling of C1q to Sepharose beads allowed the affinity precipitation of a single 125-kDa band from surface iodinated PMN. Sepharose 28-37 complement C1q A chain Homo sapiens 21-24 7964180-10 1994 When the dissociated apoA-I was subjected to agarose gel electrophoresis, it migrated to a prebeta position comparable to that of purified, lipid-free apoA-I. Sepharose 45-52 apolipoprotein A1 Homo sapiens 21-27 8011684-6 1994 Agarose gel electrophoresis revealed that Lf binds to Ac- or OxLDLs and neutralizes their negative charges. Sepharose 0-7 lactotransferrin Bos taurus 42-44 7948782-6 1994 A minimal increase in the PG synthesis was observed in NK cells; however, PG synthesized under the influence of HGF or TGF-beta 1 were of relatively higher molecular weight, with a shift of K(av) from 0.28 to 0.12, as ascertained by Sepharose-6B chromatography. Sepharose 233-242 hepatocyte growth factor Homo sapiens 112-115 7948782-6 1994 A minimal increase in the PG synthesis was observed in NK cells; however, PG synthesized under the influence of HGF or TGF-beta 1 were of relatively higher molecular weight, with a shift of K(av) from 0.28 to 0.12, as ascertained by Sepharose-6B chromatography. Sepharose 233-242 transforming growth factor beta 1 Homo sapiens 119-129 8010949-7 1994 ABP was purified to apparent homogeneity from platelets and aortic smooth muscle as a 63 kDa protein by immunoaffinity chromatography on mAb 213-21-agarose. Sepharose 148-155 sex hormone binding globulin Homo sapiens 0-3 8207004-8 1994 ZZ-tagged Pkc1p was partially purified by chromatography on DEAE-Sepharose, followed by IgG-Sepharose. Sepharose 65-74 protein kinase C Saccharomyces cerevisiae S288C 10-15 8013625-3 1994 In contrast, Mab V58A4 was shown to react with larger HBD fragments (50-60 kDa) that were present in platelet or endothelial cell extracts and could be retained on a heparin-Sepharose column at low salt concentrations. Sepharose 174-183 HBD Homo sapiens 54-57 8003509-7 1994 HIT cell ASCI-PLA2 exhibited an apparent isoelectric point of 7.5 on chromatofocusing analysis and was quantitatively adsorbed to an ATP-agarose matrix and selectively desorbed from this column by ATP. Sepharose 137-144 phospholipase A2 group IB Homo sapiens 9-18 8003509-8 1994 Mono-Q anion-exchange analysis of the active ATP-agarose eluant yielded a peak of ASCI-PLA2 activity associated with a single protein band with an apparent molecular mass of 40 kDa. Sepharose 49-56 phospholipase A2 group IB Homo sapiens 82-91 8202520-8 1994 However, affinity for heparin-agarose correlated directly with the rate of inhibition by antithrombin III with heparin. Sepharose 30-37 serpin family C member 1 Homo sapiens 89-105 8203882-5 1994 This stromelysin form apparently played a major role in Fn-f-mediated PG release, since addition of Sepharose-bound anti-stromelysin-1 to cartilage cultures greatly slowed rates of PG release. Sepharose 100-109 stromelysin-1 Bos taurus 121-134 8195236-5 1994 The secreted molecules (RrSP-D), which were purified by affinity chromatography on maltosyl-agarose, comigrated with rSP-D on SDS-polyacrylamide gel electrophoresis in the presence and absence of reduction, and coeluted with rSP-D dodecamers from 4% agarose. Sepharose 92-99 surfactant protein D Rattus norvegicus 25-30 7925074-1 1994 Glutathione S-transferase (GST) with activity towards CDNB as a substrate from human intrauterine conceptual tissues (HICT) at 6-10 weeks of gestation was purified approximately 200-fold by GSH coupled Sepharose 4B affinity chromatography. Sepharose 202-214 glutathione S-transferase kappa 1 Homo sapiens 0-25 7516153-1 1994 The interaction between bovine lipoprotein lipase (bLPL) and human alpha 2-macroglobulin (alpha 2M) was studied by use of non-denaturing PAGE and gel-permeation, Zn(2+)-Sepharose and heparin-Sepharose chromatography. Sepharose 169-178 lipoprotein lipase Homo sapiens 31-49 7516153-1 1994 The interaction between bovine lipoprotein lipase (bLPL) and human alpha 2-macroglobulin (alpha 2M) was studied by use of non-denaturing PAGE and gel-permeation, Zn(2+)-Sepharose and heparin-Sepharose chromatography. Sepharose 169-178 alpha-2-macroglobulin Homo sapiens 90-98 7516153-4 1994 Whereas alpha 2M in complex with bLPL still bound to Zn(2+)-Sepharose, bLPL lost the ability to bind to heparin-Sepharose. Sepharose 60-69 alpha-2-macroglobulin Homo sapiens 8-16 8086857-3 1994 Osteogenin was extracted from bovine bone with 6 M urea and purified by chromatography on hydroxyapatite, heparin-Sepharose and Sephacryl S-200 gel filtration. Sepharose 114-123 bone morphogenetic protein 3 Bos taurus 0-10 8086338-5 1994 Western blot analysis of growth factors revealed the presence of the M(r) 18,000, 22,000 and 24,000 forms of basic fibroblast growth factor (bFGF or FGF2) both in HBL100 cell extracts partially purified on heparin-Sepharose beads and in concentrated conditioned medium. Sepharose 214-223 fibroblast growth factor 2 Homo sapiens 141-145 7925074-1 1994 Glutathione S-transferase (GST) with activity towards CDNB as a substrate from human intrauterine conceptual tissues (HICT) at 6-10 weeks of gestation was purified approximately 200-fold by GSH coupled Sepharose 4B affinity chromatography. Sepharose 202-214 glutathione S-transferase kappa 1 Homo sapiens 27-30 7522590-2 1994 We demonstrated that differences in the percentage of prostate-specific antigen bound to concanavalin A-Sepharose in patients with benign prostatic hyperplasia compared with patients with prostatic carcinoma, as described in the literature, arise when insufficient concanavalin A binding sites are added for complete binding of the glycosylation variants of prostate-specific antigen. Sepharose 104-113 kallikrein related peptidase 3 Homo sapiens 54-79 7522590-2 1994 We demonstrated that differences in the percentage of prostate-specific antigen bound to concanavalin A-Sepharose in patients with benign prostatic hyperplasia compared with patients with prostatic carcinoma, as described in the literature, arise when insufficient concanavalin A binding sites are added for complete binding of the glycosylation variants of prostate-specific antigen. Sepharose 104-113 kallikrein related peptidase 3 Homo sapiens 358-383 7915999-6 1994 The sICAM-1, purified from the cell-free supernatant obtained after a 48 h culture of EC in IL-1 beta by affinity column chromatography using monoclonal ICAM-1 antibody coupled to Sepharose beads, significantly inhibited leukocyte-EC adhesion. Sepharose 180-189 interleukin 1 beta Homo sapiens 92-101 7915999-6 1994 The sICAM-1, purified from the cell-free supernatant obtained after a 48 h culture of EC in IL-1 beta by affinity column chromatography using monoclonal ICAM-1 antibody coupled to Sepharose beads, significantly inhibited leukocyte-EC adhesion. Sepharose 180-189 intercellular adhesion molecule 1 Homo sapiens 5-11 7959741-8 1994 A set of 39 SSR variants, resolvable by agarose gel electrophoresis and suitable for pooled-SSR analysis, is identified. Sepharose 40-47 signal sequence receptor, alpha Mus musculus 12-15 8188759-11 1994 Further studies demonstrated that monoclonal antibody against the fibrin- and heparin-binding domain at the N-terminal of plasma fibronectin prevented radiolabeled hyaluronan from binding to fibronectin; likewise, the isolated N-terminal fragment, coupled with Sepharose 4B, bound to hyaluronan in columns. Sepharose 261-273 fibronectin 1 Homo sapiens 129-140 7515388-5 1994 The median charge and degree of charge heterogeneity of the isoforms of hCG in each serum was determined by electrophoresis in 0.10% agarose suspension. Sepharose 133-140 hypertrichosis 2 (generalised, congenital) Homo sapiens 72-75 8077841-14 1994 Lysine-Sepharose chromatography of plasma yielded retained products that contained apo[a] and apoB-100 but not apoB-38.9. Sepharose 7-16 apolipoprotein B Homo sapiens 94-102 8077841-14 1994 Lysine-Sepharose chromatography of plasma yielded retained products that contained apo[a] and apoB-100 but not apoB-38.9. Sepharose 7-16 apolipoprotein B Homo sapiens 94-98 8189220-5 1994 More specifically, three distinct protein peaks eluted from the heparin-Sepharose column, two of which bound 125I-AngII with high affinity and saturability. Sepharose 72-81 angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Mus musculus 114-119 8189220-13 1994 Collectively, these results suggest that heparin-Sepharose chromatography can efficiently separate two pharmacologically, biochemically and immunologically distinct populations of AT2 receptors. Sepharose 49-58 serine (or cysteine) peptidase inhibitor, clade B, member 9d Mus musculus 180-183 7969266-7 1994 Recombinant bifunctional enzyme and the DHFR domain have been purified by methotrexate-Sepharose chromatography to yield 1-2 mg of active DHFR-TS per litre of culture. Sepharose 87-96 dihydrofolate reductase Escherichia coli 40-44 8052513-1 1994 Acetylcholinesterase (AChE) has been purified from the excretory/secretory (ES) products of Trichostrongylus colubriformis (using edrophonium chloride linked to epoxy-activated Sepharose) with yields of 40-50%. Sepharose 177-186 acetylcholinesterase Ovis aries 22-26 7950373-1 1994 Human cholesteryl ester transfer protein was purified from lipoprotein-depleted serum or plasma in a three-step procedure utilizing commercially available triazine dyes immobilized on agarose. Sepharose 184-191 cholesteryl ester transfer protein Homo sapiens 6-40 8195168-3 1994 p20 was separated from alpha B crystallin and HSP27/28 and was resolved into two fractions, a minor first peak and a major second peak, by column chromatography on S-Sepharose in the presence of 7 M urea. Sepharose 166-175 tubulin polymerization promoting protein family member 3 Homo sapiens 0-3 8198596-3 1994 These PKC gamma overexpressing cell clones show an increased rate of growth in monolayer culture, increased colony-forming efficiency on soft agarose, and increased DNA synthesis in response to epidermal growth factor and basic fibroblast growth factor. Sepharose 142-149 protein kinase C gamma Homo sapiens 6-15 8186264-6 1994 Investigating the novel t-PA binding to casein micelles by ligand blotting and Sepharose immobilized casein, multimeric forms of kappa-casein and dimeric alpha s2-casein were identified as t-PA binding components. Sepharose 79-88 plasminogen activator, tissue type Bos taurus 24-28 8182050-6 1994 Affinity chromatography on heparin-Sepharose showed that RMCP-1 displayed high affinity for heparin, with approximately 1.2 M NaCl being required for elution of RMCP-1 from the affinity matrix. Sepharose 35-44 mast cell protease 1-like 1 Rattus norvegicus 57-63 7764847-5 1994 After partial purification of inclusion bodies and subject to the HA-cleavage treatment, gag protein was further purified to homogeneity using DEAE-Sepharose chromatography. Sepharose 148-157 Pr55(Gag) Human immunodeficiency virus 1 89-92 8168085-6 1994 Agarose gel electrophoresis of DNA extracted from irradiated bovine aortic endothelial cells showed that both TPA (30 ng/ml; 30 min) and bFGF (1 ng/ml) inhibited the apoptotic degradation of DNA induced in these cells by radiation exposure (500 cGy). Sepharose 0-7 fibroblast growth factor 2 Bos taurus 137-141 8181864-4 1994 IFN alpha/beta treatment resulted in 50- to 100-fold inhibition of FLC multiplication in the liver and spleen of normal DBA/2 mice shortly after tumor inoculation, as evaluated by colony formation in agarose. Sepharose 200-207 interferon alpha Mus musculus 0-9 8198542-6 1994 Oleic acid at 200 microM also effectively displaced plasmin prebound to a polylysine-Sepharose column. Sepharose 85-94 plasminogen Homo sapiens 52-59 8188683-6 1994 NBD-TAP1 bound to C-8-ATP-agarose and was specifically eluted with ATP or EDTA. Sepharose 26-33 transporter 1, ATP binding cassette subfamily B member Homo sapiens 4-8 8080958-2 1994 In the vulnerable CA3 region of the rat hippocampus, neurones developed apoptotic properties since, (i) their nuclei were positively stained with a selective in situ DNA fragmentation staining method; (ii) DNA cleavage into internucleosome-sized fragments was observed on agarose gels. Sepharose 272-279 carbonic anhydrase 3 Rattus norvegicus 18-21 8175758-4 1994 The p15cdk-BP-bound "PSTAIRE signal" is part of a 250-kDa complex distinct from p34cdc2/cyclin B. p15cdk-BP-Sepharose beads retain a kinase phosphorylating HMG I/Y, P1, and myelin basic protein (among 24 substrates tested). Sepharose 108-117 cyclin dependent kinase 1 Homo sapiens 80-87 8175758-4 1994 The p15cdk-BP-bound "PSTAIRE signal" is part of a 250-kDa complex distinct from p34cdc2/cyclin B. p15cdk-BP-Sepharose beads retain a kinase phosphorylating HMG I/Y, P1, and myelin basic protein (among 24 substrates tested). Sepharose 108-117 high mobility group AT-hook 1 Homo sapiens 156-163 8175758-4 1994 The p15cdk-BP-bound "PSTAIRE signal" is part of a 250-kDa complex distinct from p34cdc2/cyclin B. p15cdk-BP-Sepharose beads retain a kinase phosphorylating HMG I/Y, P1, and myelin basic protein (among 24 substrates tested). Sepharose 108-117 myelin basic protein Homo sapiens 173-193 8175758-9 1994 cdc2 and cdk2 bind to p9CKShs1-Sepharose, but not to p15cdk-BP. Sepharose 31-40 cyclin dependent kinase 1 Homo sapiens 0-4 8175758-9 1994 cdc2 and cdk2 bind to p9CKShs1-Sepharose, but not to p15cdk-BP. Sepharose 31-40 cyclin dependent kinase 2 Homo sapiens 9-13 8175758-10 1994 cdk4 and cdk5 bind to p15cdk-BP-Sepharose, but not to p9CKShs1-Sepharose. Sepharose 32-41 cyclin dependent kinase 4 Homo sapiens 0-4 8175758-10 1994 cdk4 and cdk5 bind to p15cdk-BP-Sepharose, but not to p9CKShs1-Sepharose. Sepharose 32-41 cyclin dependent kinase 5 Homo sapiens 9-13 8074289-4 1994 [3H]BANA-CCK-8s was bound to a streptavidin-agarose affinity matrix and was subsequently released by irradiation with uv light of wavelengths > 320 nm. Sepharose 44-51 cholecystokinin Homo sapiens 9-12 8172858-8 1994 PAI-1 stimulatory activity in SMC CM was completely abolished by boiling or incubation with protamine sulfate and was reduced by transient acidification or heparin-Sepharose pretreatment by 33% or 48%, respectively. Sepharose 164-173 serpin family E member 1 Homo sapiens 0-5 8049156-3 1994 The major binding proteins and substrates in REF52 cells shared similar properties including enrichment by calmodulin-Sepharose chromatography, binding to phosphatidylserine, and resistance to heat denaturation. Sepharose 118-127 calmodulin 1 Rattus norvegicus 107-117 8075524-1 1994 A method for the preparation of a p-aminobenzene sulphonyl ethyl containing crosslinked Sepharose 4B (ABSE-Sepharose 4B-CL) is described, trypsin, bovine serum albumin (BSA) and concanavalin A (Con A) were immobilized onto this matrix by diazotization. Sepharose 88-100 albumin Homo sapiens 154-167 7514531-4 1994 We have used Red A Sepharose- and sequence-specific DNA affinity chromatography to purify MGF from mammary gland tissue of lactating sheep. Sepharose 19-28 signal transducer and activator of transcription 5A Ovis aries 90-93 8088314-2 1994 Recently Lp(a) was fractionated into two species with different affinities for Lysine-Sepharose. Sepharose 86-95 lipoprotein(a) Homo sapiens 9-14 8166722-2 1994 An expression plasmid encoding the extracellular plasma region of the human EPO-R (sEPO-R) was transfected into COS cells, and the sEPO-R so produced was labelled with [35S]methionine and purified by EPO-affinity chromatography on a EAH-Sepharose 4B-EPO column. Sepharose 237-249 erythropoietin receptor Homo sapiens 76-81 7921790-8 1994 Further fractionation of the HDL2-HDL3 bound activity on heparin-agarose established that 70% of the recovered activity was bound to the apo-E containing HDL. Sepharose 65-72 high density lipoprotein (HDL) level 2 Mus musculus 29-33 7921790-8 1994 Further fractionation of the HDL2-HDL3 bound activity on heparin-agarose established that 70% of the recovered activity was bound to the apo-E containing HDL. Sepharose 65-72 high density lipoprotein (HDL) level 3 Mus musculus 34-38 7921790-8 1994 Further fractionation of the HDL2-HDL3 bound activity on heparin-agarose established that 70% of the recovered activity was bound to the apo-E containing HDL. Sepharose 65-72 apolipoprotein E Mus musculus 137-142 8079446-5 1994 Stable form of the high molecular angiotensin-converting enzyme, which did not transform into its low molecular derivative, was obtained after treatment with agarose-immobilized aprotinin. Sepharose 158-165 pancreatic trypsin inhibitor Bos taurus 178-187 8175664-2 1994 As expected, it binds with high affinity to heparin-Sepharose like FGF-2 and can displace the binding of radiolabeled FGF-2 to its high affinity receptor. Sepharose 52-61 fibroblast growth factor 2 Mus musculus 67-72 8175664-2 1994 As expected, it binds with high affinity to heparin-Sepharose like FGF-2 and can displace the binding of radiolabeled FGF-2 to its high affinity receptor. Sepharose 52-61 fibroblast growth factor 2 Mus musculus 118-123 8185227-5 1994 GST-huEPO eluted from glutathione-agarose using reduced glutathione (GSH) was tested by radioimmunoassay and in a mouse spleen cell assay (MSCA). Sepharose 34-41 hematopoietic prostaglandin D synthase Mus musculus 0-3 7512561-1 1994 hGBP1 is an interferon-induced 67-kDa protein of human cells that readily binds to agarose-immobilized GTP, GDP, and GMP but not to other nucleotides. Sepharose 83-90 guanylate binding protein 1 Homo sapiens 0-5 8159751-6 1994 TFPI-2 was expressed in baby hamster kidney cells and purified from the serum-free conditioned medium by a combination of heparin-agarose chromatography, Mono Q FPLC, Mono S FPLC, and Superose 12 FPLC. Sepharose 130-137 tissue factor pathway inhibitor 2 Homo sapiens 0-6 8157662-5 1994 The LBR amino-terminal domain precipitates lamin B from nuclear extracts and retards the migration of double-stranded DNA subjected to agarose gel electrophoresis. Sepharose 135-142 lamin B receptor Homo sapiens 4-7 8053565-2 1994 The histidine-tagged protein A bound efficiently to iminodiacetic acid (IDA)-Sepharose loaded with Zn2+, and the GST-protein A was efficiently retained by glutathione-Sepharose. Sepharose 167-176 glutathione S-transferase kappa 1 Homo sapiens 113-116 8161225-3 1994 The mouse mitochondrial matrix GST was purified using a combination of (NH4)2SO4 fractionation, Sephadex gel filtration and affinity chromatography on glutathione (GSH) conjugated Sepharose. Sepharose 180-189 hematopoietic prostaglandin D synthase Mus musculus 31-34 8069230-5 1994 IR and Gir from human placental membrane bound to insulin-Sepharose column. Sepharose 58-67 insulin receptor Homo sapiens 0-2 8069230-5 1994 IR and Gir from human placental membrane bound to insulin-Sepharose column. Sepharose 58-67 G protein-coupled receptor 83 Homo sapiens 7-10 8060533-5 1994 TNF-alpha receptor was purified from detergent-solubilized rat Kupffer cells by adsorption to recombinant human TNF-alpha-Sepharose. Sepharose 122-131 tumor necrosis factor Rattus norvegicus 0-9 8069230-5 1994 IR and Gir from human placental membrane bound to insulin-Sepharose column. Sepharose 58-67 insulin Homo sapiens 50-57 8069230-7 1994 Both IR and Gir, thus eluted, absorbed on the anti-IR-Sepharose column and GTP eluted Gir. Sepharose 54-63 insulin receptor Homo sapiens 5-7 8069230-7 1994 Both IR and Gir, thus eluted, absorbed on the anti-IR-Sepharose column and GTP eluted Gir. Sepharose 54-63 G protein-coupled receptor 83 Homo sapiens 12-15 8060533-5 1994 TNF-alpha receptor was purified from detergent-solubilized rat Kupffer cells by adsorption to recombinant human TNF-alpha-Sepharose. Sepharose 122-131 tumor necrosis factor Homo sapiens 112-121 8033590-1 1994 Intracellular binding proteins have been identified and isolated from B cells by their ability to bind to the synthetic peptide (1169-1191), the major immunodominant epitope of bovine interphotoreceptor retinoid-binding protein (IRBP) coupled to cyanogen bromide activated Sepharose 4B. Sepharose 273-282 retinol binding protein 3 Bos taurus 184-227 8156688-2 1994 Transforming growth factor-beta 1 (TGF-beta 1) has been shown to stimulate chondrogenesis in periosteal explants cultured in agarose suspension. Sepharose 125-132 LOW QUALITY PROTEIN: transforming growth factor beta-1 Oryctolagus cuniculus 0-33 8156688-2 1994 Transforming growth factor-beta 1 (TGF-beta 1) has been shown to stimulate chondrogenesis in periosteal explants cultured in agarose suspension. Sepharose 125-132 LOW QUALITY PROTEIN: transforming growth factor beta-1 Oryctolagus cuniculus 35-45 8156688-4 1994 Periosteal explants and fascia lata were harvested from two-month-old rabbits, cultured for six weeks with 0, 0.1, 1, 5, 10, 50, or 100 ng/mL TGF-beta 1 in agarose suspension, then analyzed by histomorphometry and quantitative collagen typing. Sepharose 156-163 LOW QUALITY PROTEIN: transforming growth factor beta-1 Oryctolagus cuniculus 142-152 8033590-1 1994 Intracellular binding proteins have been identified and isolated from B cells by their ability to bind to the synthetic peptide (1169-1191), the major immunodominant epitope of bovine interphotoreceptor retinoid-binding protein (IRBP) coupled to cyanogen bromide activated Sepharose 4B. Sepharose 273-282 retinol binding protein 3 Bos taurus 229-233 7921655-8 1994 MGSA was cleaved from the complex of fusion protein and glutathione-sepharose beads with thrombin and purified to homogeneity by anion-exchange high-performance liquid chromatography with a Mono-S-column. Sepharose 68-77 C-X-C motif chemokine ligand 1 Homo sapiens 0-4 7915252-8 1994 sICAM-1, purified from cell-free supernatants obtained after a 48-h culture of EC in IL-1 beta by affinity chromatography using monoclonal ICAM-1 antibody coupled to Sepharose beads, significantly inhibited lymphocyte EC adhesion. Sepharose 166-175 interleukin 1 beta Homo sapiens 85-94 7988920-2 1994 The purity and specificity of isolated transcortin was tested by agarose gel electrophoresis using racket and cross immunoelectrophoresis and specific CBG antibodies. Sepharose 65-72 serpin family A member 6 Homo sapiens 39-50 7927439-1 1994 The 14 kDa beta-galactoside-binding lectin from bovine brain grey matter (BBL) covalently attached to caproic acid-Sepharose by the N-hydroxy succinimide procedure was used to isolate endogenous glycoprotein receptors of this lectin. Sepharose 115-124 galactoside-binding soluble lectin 13 Bos taurus 11-42 7915252-8 1994 sICAM-1, purified from cell-free supernatants obtained after a 48-h culture of EC in IL-1 beta by affinity chromatography using monoclonal ICAM-1 antibody coupled to Sepharose beads, significantly inhibited lymphocyte EC adhesion. Sepharose 166-175 intercellular adhesion molecule 1 Homo sapiens 1-7 8144498-1 1994 A previously undescribed high molecular mass protein (HMP) from human erythrocyte membranes was solubilized by Triton X-100 and purified on a calmodulin-agarose column in the presence of Ca2+. Sepharose 153-160 inner membrane mitochondrial protein Homo sapiens 25-52 8144540-10 1994 When tested on fibrin-Sepharose, only the bimodular fragment rF4-5 was able to bind. Sepharose 22-31 Renal function QTL 45 Rattus norvegicus 61-66 8144498-1 1994 A previously undescribed high molecular mass protein (HMP) from human erythrocyte membranes was solubilized by Triton X-100 and purified on a calmodulin-agarose column in the presence of Ca2+. Sepharose 153-160 inner membrane mitochondrial protein Homo sapiens 54-57 8144523-8 1994 Using heparin-agarose affinity chromatography, a 116-kDa LPL-binding protein was purified from endothelial cell extracts. Sepharose 14-21 lipoprotein lipase Homo sapiens 57-60 7511169-4 1994 Protease-sensitive PrP released from the surface of mouse neuroblastoma cells bound to heparin-agarose and Congo red-glass beads. Sepharose 95-102 prion protein Mus musculus 19-22 8163669-8 1994 However, HuCIITg VLDL showed markedly decreased binding to heparin-Sepharose, suggesting that apoCII-rich, apoE-poor lipoprotein may be less accessible to cell surface lipases or receptors within their glycosaminoglycan matrices. Sepharose 67-76 apolipoprotein C2 Mus musculus 94-100 8163669-8 1994 However, HuCIITg VLDL showed markedly decreased binding to heparin-Sepharose, suggesting that apoCII-rich, apoE-poor lipoprotein may be less accessible to cell surface lipases or receptors within their glycosaminoglycan matrices. Sepharose 67-76 apolipoprotein E Mus musculus 107-111 8110198-1 1994 Neutrophil-membrane-associated NADPH-cytochrome c reductase and cytochrome b558 were separately eluted and highly purified by a combination of ion-exchange Sepharose, N-amino-octylagarose, 2",5"-ADP-Sepharose and heparin-Sepharose column chromatographies. Sepharose 199-208 mitochondrially encoded cytochrome b Homo sapiens 64-76 8143454-1 1994 Fibronectin from human early pregnancy (5-8 weeks) placenta (epFN) has been isolated by 2M urea-PBS extraction and purified by heparin-Sepharose 4B affinity chromatography followed by Sepharose CL-6B gel filtration, and compared with that of term placenta (tpFN). Sepharose 135-144 fibronectin 1 Homo sapiens 0-11 8179823-6 1994 Immunization of rabbits with the GST/CP-10 fusion protein bound to glutathione-agarose beads resulted in high titer, specific antibodies that neutralized CP-10-initiated chemotaxis and were suitable for immunoblotting. Sepharose 79-86 S100 calcium binding protein A8 (calgranulin A) Mus musculus 37-42 8179823-6 1994 Immunization of rabbits with the GST/CP-10 fusion protein bound to glutathione-agarose beads resulted in high titer, specific antibodies that neutralized CP-10-initiated chemotaxis and were suitable for immunoblotting. Sepharose 79-86 S100 calcium binding protein A8 (calgranulin A) Mus musculus 154-159 7515696-1 1994 To study the relationship between a bovine serum mannan-binding protein (MBP) and a serum protein reactive with a Ra chemotype strain of Salmonella serovar Typhimurium (Ra-reactive factor, RaRF), both proteins were isolated by use of their affinity for yeast mannan and the Salmonella cells followed by affinity chromatography on mannobiose-Sepharose 4B. Sepharose 341-350 myelin basic protein Homo sapiens 49-71 7515696-1 1994 To study the relationship between a bovine serum mannan-binding protein (MBP) and a serum protein reactive with a Ra chemotype strain of Salmonella serovar Typhimurium (Ra-reactive factor, RaRF), both proteins were isolated by use of their affinity for yeast mannan and the Salmonella cells followed by affinity chromatography on mannobiose-Sepharose 4B. Sepharose 341-350 myelin basic protein Bos taurus 73-76 8170475-9 1994 Hsp70 coeluted with hGR from a glucocorticoid response element (GRE)-Sepharose column, suggesting that hsp70 is part of the GRE-hGR complex. Sepharose 69-78 heat shock protein family A (Hsp70) member 4 Homo sapiens 0-5 8170475-9 1994 Hsp70 coeluted with hGR from a glucocorticoid response element (GRE)-Sepharose column, suggesting that hsp70 is part of the GRE-hGR complex. Sepharose 69-78 heat shock protein family A (Hsp70) member 4 Homo sapiens 103-108 8190711-1 1994 A method involving direct affinity chromatography of undialysed bovine seminal plasma on a calmodulin-agarose column was developed for the purification of seminalplasmin. Sepharose 102-109 caltrin Bos taurus 155-169 8190711-2 1994 Seminalplasmin thus obtained was further purified from any contaminants left by ion-exchange chromatography on a short CM-Sepharose column. Sepharose 122-131 caltrin Bos taurus 0-14 8294420-2 1994 Phosphodiester alpha-GlcNAcase, a membrane-bound enzyme, has been purified about 3,000-fold from bovine liver to apparent homogeneity using detergent solubilization, fractionation on DEAE-cellulose, affinity chromatography on lectin-Sepharose columns, gel filtration, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 233-242 N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase Bos taurus 0-30 8307033-3 1994 UCP was purified by means of specific polyclonal antibodies immobilized on protein-A--agarose. Sepharose 86-93 uncoupling protein 1 Rattus norvegicus 0-3 8278410-2 1994 Exposure of these cells to tumor necrosis factor alpha resulted in internucleosomal cleavage of genomic DNA, yielding laddered patterns of oligonucleosomal fragments characteristic of apoptosis when resolved by agarose gel electrophoresis; similar responses were observed after exposure to exogenous sphingomyelinase or synthetic ceramides. Sepharose 211-218 tumor necrosis factor Homo sapiens 27-54 7509643-1 1994 Using plasminogen-Sepharose 4B chromatography, a 50-70% reduction in the alpha-2-antiplasmin (alpha-2-AP) content in human and dog blood plasma was reached. Sepharose 18-27 serpin family F member 2 Homo sapiens 73-92 7509643-1 1994 Using plasminogen-Sepharose 4B chromatography, a 50-70% reduction in the alpha-2-antiplasmin (alpha-2-AP) content in human and dog blood plasma was reached. Sepharose 18-27 serpin family F member 2 Homo sapiens 94-104 7509643-4 1994 The decrease in the fibrinogen concentration in human blood plasma caused by plasmosorption on plasminogen-Sepharose enhanced the model fibrin clot lysis by urokinase and streptokinase, however, by no more than 5-10%. Sepharose 107-116 fibrinogen beta chain Homo sapiens 20-30 7510554-5 1994 The conjugate purified by the use of Blue Sepharose showed selective cytotoxicity against AFP-producing human hepatoma cells. Sepharose 42-51 alpha fetoprotein Homo sapiens 90-93 7912672-3 1994 EPF has been purified from clinically outdated human platelets by heat extraction, ion-exchange and affinity chromatographies on SP-Sephadex and heparin-Sepharose respectively, high-performance hydrophobic interaction chromatography and three reverse-phase HPLC steps, with an average yield of 15 micrograms/100 platelet units (equivalent to approximately 50 1 blood). Sepharose 153-162 heat shock protein family E (Hsp10) member 1 Homo sapiens 0-3 7935515-1 1994 A deazaguanine-substituted DNA PCR product from FMR-1 (the fragile X mental retardation syndrome gene) can be efficiently visualized with ethidium bromide on standard agarose gels. Sepharose 167-174 fragile X messenger ribonucleoprotein 1 Homo sapiens 48-53 8054845-3 1994 However, the Lens culinaris lectin immobilized on Sepharose 4B (LCA-Sepharose) provided a more selective and flexible affinity system for the purification of FVIII/vWF than Concanavalia lectin. Sepharose 50-62 coagulation factor VIII Homo sapiens 158-163 8054845-3 1994 However, the Lens culinaris lectin immobilized on Sepharose 4B (LCA-Sepharose) provided a more selective and flexible affinity system for the purification of FVIII/vWF than Concanavalia lectin. Sepharose 50-62 von Willebrand factor Homo sapiens 164-167 8054845-3 1994 However, the Lens culinaris lectin immobilized on Sepharose 4B (LCA-Sepharose) provided a more selective and flexible affinity system for the purification of FVIII/vWF than Concanavalia lectin. Sepharose 50-59 coagulation factor VIII Homo sapiens 158-163 8054845-3 1994 However, the Lens culinaris lectin immobilized on Sepharose 4B (LCA-Sepharose) provided a more selective and flexible affinity system for the purification of FVIII/vWF than Concanavalia lectin. Sepharose 50-59 von Willebrand factor Homo sapiens 164-167 8054845-4 1994 Chromatography on LCA-Sepharose of a purified FVIII containing a small proportion of vWF required a weak acidic medium (pH 6.3) and relatively slow kinetics (about 20 cm/h flow rate). Sepharose 22-31 coagulation factor VIII Homo sapiens 46-51 8054845-4 1994 Chromatography on LCA-Sepharose of a purified FVIII containing a small proportion of vWF required a weak acidic medium (pH 6.3) and relatively slow kinetics (about 20 cm/h flow rate). Sepharose 22-31 von Willebrand factor Homo sapiens 85-88 8054845-5 1994 The bound FVIII was specifically dissociated from LCA-Sepharose by methyl-alpha-D-mannopyranoside, and to a lesser extent by other monosaccharides such as D-glucose, methyl-alpha-D-glucopyranoside, D-mannose, and D-galactose. Sepharose 54-63 coagulation factor VIII Homo sapiens 10-15 8054846-0 1994 Catalysis of protein folding by agarose-immobilized protein disulfide isomerase. Sepharose 32-39 prolyl 4-hydroxylase subunit beta Homo sapiens 52-79 8054846-2 1994 PDI coupled to cyanogen bromide-activated agarose retains its catalytic activity, and a column of this material increases both the rate and the yield for folding disulfide-containing proteins. Sepharose 42-49 prolyl 4-hydroxylase subunit beta Homo sapiens 0-3 8054853-2 1994 The glutathione-S-transferase:calretinin fusion protein produced in Escherichia coli was purified on a glutathione-Sepharose affinity column. Sepharose 115-124 calbindin 2 Rattus norvegicus 30-40 8052970-2 1994 Thrombin adsorbed to either sulfopropyl-Sephadex or heparin-Sepharose bound > 95% of 125I-r-hirudin and the complex remained bound to the matrix. Sepharose 60-69 prothrombin Oryctolagus cuniculus 0-8 7969099-11 1994 The results of this study demonstrate that GR is an effective substrate for action of cAMP-PK and that the immunopurified protein A-Sepharose adsorbed GR lacks intrinsic kinase activity but can be conveniently used for the characterization of the phosphorylation reaction in the presence of an exogenous kinase. Sepharose 132-141 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 151-153 7510698-8 1994 In addition, angiogenin binds tightly to heparin-Sepharose, requiring 0.78 M NaCl for elution. Sepharose 49-58 angiogenin Homo sapiens 13-23 8132633-4 1994 Quantitative analysis of electrophoretic mobilities in agarose gels indicates that the surface potentials of reconstituted pre-beta HDL and alpha-HDL3 are -7.6 and -11.4 mV, respectively. Sepharose 55-62 HDL3 Homo sapiens 146-150 8132639-5 1994 The CMP-NeuAc hydroxylase was purified to homogeneity from the cytosolic fraction of mouse liver, using ion exchange columns, a Red-Sepharose column, and a soluble cytochrome b5-immobilized Sepharose column. Sepharose 132-141 cytidine monophospho-N-acetylneuraminic acid hydroxylase Mus musculus 4-25 8132639-5 1994 The CMP-NeuAc hydroxylase was purified to homogeneity from the cytosolic fraction of mouse liver, using ion exchange columns, a Red-Sepharose column, and a soluble cytochrome b5-immobilized Sepharose column. Sepharose 190-199 cytidine monophospho-N-acetylneuraminic acid hydroxylase Mus musculus 4-25 8132639-5 1994 The CMP-NeuAc hydroxylase was purified to homogeneity from the cytosolic fraction of mouse liver, using ion exchange columns, a Red-Sepharose column, and a soluble cytochrome b5-immobilized Sepharose column. Sepharose 190-199 cytochrome b5 type A (microsomal) Mus musculus 164-177 8132538-1 1994 We measured Ca(2+)-calmodulin binding to expressed human caldesmon fragments by three techniques: tryptophan fluorescence enhancement, change in fluorescence of TA-calmodulin, and cosedimentation with calmodulin-Sepharose. Sepharose 212-221 calmodulin 1 Homo sapiens 19-29 8132576-1 1994 In an effort to understand the relationship between a 72-kDa heat shock protein (Hsp72) and peroxisome proliferator-activated receptors (PPARs), we have characterized their interaction using clofibric acid-Sepharose chromatography and co-immunoprecipitation with antisera raised against either rat PPAR (rPPAR) or Hsp72. Sepharose 206-215 heat shock protein family A (Hsp70) member 1A Rattus norvegicus 81-86 8132576-2 1994 First, we observed that both rPPAR and Hsp72 elute in a clofibrate-dependent manner from the clofibric acid-Sepharose matrix. Sepharose 108-117 peroxisome proliferator activated receptor alpha Rattus norvegicus 29-34 8132576-2 1994 First, we observed that both rPPAR and Hsp72 elute in a clofibrate-dependent manner from the clofibric acid-Sepharose matrix. Sepharose 108-117 heat shock protein family A (Hsp70) member 1A Rattus norvegicus 39-44 7511628-8 1994 The active TcsF that bound to anti-TCR-alpha but not to the anti-TCR-beta column was shown subsequently to have the ability to bind to specific Ag columns TNP-BSA and TNP-BGG Sepharose 4B. Sepharose 175-184 basigin (Ok blood group) Homo sapiens 11-15 7511628-8 1994 The active TcsF that bound to anti-TCR-alpha but not to the anti-TCR-beta column was shown subsequently to have the ability to bind to specific Ag columns TNP-BSA and TNP-BGG Sepharose 4B. Sepharose 175-184 T cell receptor alpha constant Homo sapiens 35-44 8143719-5 1994 The maximal velocity at high pH becomes insensitive to both calmodulin and controlled proteolysis, although calmodulin binds to the protein with similar affinities at pH 7.0 and 8.0, as indicated by similarity in binding to a calmodulin-Sepharose resin and in dependence on calmodulin concentrations when the pH is increased. Sepharose 237-246 calmodulin 1 Homo sapiens 108-118 8143719-5 1994 The maximal velocity at high pH becomes insensitive to both calmodulin and controlled proteolysis, although calmodulin binds to the protein with similar affinities at pH 7.0 and 8.0, as indicated by similarity in binding to a calmodulin-Sepharose resin and in dependence on calmodulin concentrations when the pH is increased. Sepharose 237-246 calmodulin 1 Homo sapiens 108-118 8143719-5 1994 The maximal velocity at high pH becomes insensitive to both calmodulin and controlled proteolysis, although calmodulin binds to the protein with similar affinities at pH 7.0 and 8.0, as indicated by similarity in binding to a calmodulin-Sepharose resin and in dependence on calmodulin concentrations when the pH is increased. Sepharose 237-246 calmodulin 1 Homo sapiens 108-118 8160198-8 1994 Anaerobic solutions of SIN-1 in phosphate buffer, pH 7.4, released NO to the gas phase, where it was identified by trapping it with hemoglobin on agarose beads and deriving the characteristic NO-hemoglobin EPR spectrum. Sepharose 146-153 MAPK associated protein 1 Homo sapiens 23-28 8120033-3 1994 We purified IP3R-I from a 1% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid solubilized mouse cerebellar microsomal fraction by immunoaffinity chromatography on an anti-pep 6 antibody-Sepharose 4B column with specific elution by the pep 6 peptide (GHPPHMNVNPQQ) of the IP3R-I C terminus. Sepharose 199-208 inositol 1,4,5-trisphosphate receptor 1 Mus musculus 12-16 8123667-1 1994 Calmodulin (lysine 115) N-methyltransferase was purified from the cytosolic fraction of Paramecium tetraurelia by sequential dialysis, cellulose phosphate chromatography, Reactive Red 120 agarose chromatography, and calmodulin-Sepharose affinity chromatography. Sepharose 188-195 calmodulin 1 Homo sapiens 0-10 8123667-1 1994 Calmodulin (lysine 115) N-methyltransferase was purified from the cytosolic fraction of Paramecium tetraurelia by sequential dialysis, cellulose phosphate chromatography, Reactive Red 120 agarose chromatography, and calmodulin-Sepharose affinity chromatography. Sepharose 227-236 calmodulin 1 Homo sapiens 0-10 8117201-8 1994 Intramuscular implantation of bFGF in heparin-sepharose pellets at the time of arterial ligation markedly enhanced the blood flow for 3 weeks compared with untreated ischaemic limbs. Sepharose 46-55 fibroblast growth factor 2 Homo sapiens 30-34 8129795-6 1994 RESULTS: Human chondrocytes in agarose culture expressed messenger RNA (mRNA) for the IL-6 receptor (gp80) and its signal-transducing subunit gp130. Sepharose 31-38 interleukin 6 Homo sapiens 86-90 8129795-6 1994 RESULTS: Human chondrocytes in agarose culture expressed messenger RNA (mRNA) for the IL-6 receptor (gp80) and its signal-transducing subunit gp130. Sepharose 31-38 interleukin 6 receptor Homo sapiens 101-105 8129795-6 1994 RESULTS: Human chondrocytes in agarose culture expressed messenger RNA (mRNA) for the IL-6 receptor (gp80) and its signal-transducing subunit gp130. Sepharose 31-38 interleukin 6 cytokine family signal transducer Homo sapiens 142-147 8019501-1 1994 In order to identify the membrane-bound peptidase that is responsible for the degradation of endothelin (ET), an endothelin-1 (ET-1) degradation enzyme was solubilized from membrane fractions of porcine kidney with 1% Triton X-100, and subsequently purified by column chromatographies, i.e., diethylamino-Sepharose ion exchange, gel permeation, Con A Sepharose and hydroxyapatite chromatography. Sepharose 305-314 endothelin 1 Homo sapiens 113-125 8019501-1 1994 In order to identify the membrane-bound peptidase that is responsible for the degradation of endothelin (ET), an endothelin-1 (ET-1) degradation enzyme was solubilized from membrane fractions of porcine kidney with 1% Triton X-100, and subsequently purified by column chromatographies, i.e., diethylamino-Sepharose ion exchange, gel permeation, Con A Sepharose and hydroxyapatite chromatography. Sepharose 305-314 endothelin 1 Homo sapiens 127-131 8019501-1 1994 In order to identify the membrane-bound peptidase that is responsible for the degradation of endothelin (ET), an endothelin-1 (ET-1) degradation enzyme was solubilized from membrane fractions of porcine kidney with 1% Triton X-100, and subsequently purified by column chromatographies, i.e., diethylamino-Sepharose ion exchange, gel permeation, Con A Sepharose and hydroxyapatite chromatography. Sepharose 351-360 endothelin 1 Homo sapiens 113-125 8019501-1 1994 In order to identify the membrane-bound peptidase that is responsible for the degradation of endothelin (ET), an endothelin-1 (ET-1) degradation enzyme was solubilized from membrane fractions of porcine kidney with 1% Triton X-100, and subsequently purified by column chromatographies, i.e., diethylamino-Sepharose ion exchange, gel permeation, Con A Sepharose and hydroxyapatite chromatography. Sepharose 351-360 endothelin 1 Homo sapiens 127-131 8069777-8 1994 Preliminary studies indicate that the presence of a threonine at this position may enhance the interaction of Lp(a) with lysine-Sepharose. Sepharose 128-137 lipoprotein(a) Homo sapiens 110-115 8125130-8 1994 The latter was also immunoprecipitated with anti-Fc-Sepharose from the pepsin digestion supernatants of 14C-labeled-C3b-IgG complexes. Sepharose 52-61 complement C3 Homo sapiens 116-119 8206511-3 1994 Sepharose-coupled, but not streptavidin-cross-linked, plastic-adsorbed or soluble, anti-mu up-regulated the expression of IL-2R alpha and beta chains and mRNA to levels comparable to those seen in activated T cells. Sepharose 0-9 interleukin 2 receptor, alpha chain Mus musculus 122-133 8113672-6 1994 TGF-beta at concentrations > or = 10(-12) M abrogated the survival-prolonging effects of hematopoietins in a dose-dependent manner and induced apoptosis as determined by DNA fragmentation in agarose gels. Sepharose 194-201 transforming growth factor beta 1 Homo sapiens 0-8 8126463-4 1994 Large-scale production of E. coli-derived Nef 27 and Nef 25 was carried out by growing recombinant cells in a fermenter under fed-batch conditions followed by affinity purification on glutathione-Sepharose before and after thrombin cleavage. Sepharose 196-205 S100 calcium binding protein B Homo sapiens 42-45 8120612-10 1994 Following solubilization by detergents, gelsolin in the myelin fraction could be purified using anion exchange and blue Sepharose column chromatographies. Sepharose 120-129 gelsolin Rattus norvegicus 40-48 7510414-0 1994 Formation of the terminal complement complex on agarose beads: further evidence that vitronectin (complement S-protein) inhibits C9 polymerization. Sepharose 48-55 vitronectin Homo sapiens 85-96 7510414-0 1994 Formation of the terminal complement complex on agarose beads: further evidence that vitronectin (complement S-protein) inhibits C9 polymerization. Sepharose 48-55 vitronectin Homo sapiens 98-118 7510414-3 1994 The authors wished to add to this background by studying the effect of vitronectin on formation of TCC on a carbohydrate surface like agarose beads, an alternative complement pathway activator. Sepharose 134-141 vitronectin Homo sapiens 71-82 8119904-10 1994 Mature IP3R was bound by concanavalin A-Sepharose beads, and binding was greatly reduced by endoglycosidase H treatment of the receptor. Sepharose 40-49 inositol 1,4,5-trisphosphate receptor, type 1 Rattus norvegicus 7-11 8119932-1 1994 Lipoprotein lipase (LPL) was obtained from rat postheparin plasma by chromatographies on heparin-Sepharose and hydroxyapatite. Sepharose 97-106 lipoprotein lipase Rattus norvegicus 0-18 8119932-1 1994 Lipoprotein lipase (LPL) was obtained from rat postheparin plasma by chromatographies on heparin-Sepharose and hydroxyapatite. Sepharose 97-106 lipoprotein lipase Rattus norvegicus 20-23 8119969-1 1994 Recombinant human factor VIII (fVIII) was activated by thrombin at pH 7.4, followed by CM-Sepharose chromatography at pH values ranging from 3.5 to 7.4. Sepharose 90-99 coagulation factor VIII Homo sapiens 18-29 8119969-1 1994 Recombinant human factor VIII (fVIII) was activated by thrombin at pH 7.4, followed by CM-Sepharose chromatography at pH values ranging from 3.5 to 7.4. Sepharose 90-99 coagulation factor VIII Homo sapiens 31-36 8133076-5 1994 The complexes are swept out of solution using sepharose beads to which polyclonal anti-TNF antibodies have been affixed. Sepharose 46-55 tumor necrosis factor Homo sapiens 87-90 8035775-7 1994 Isolation of MAP-2 isoforms together with MAP-3 was achieved on the basis of their selective interactions with calmodulin-agarose affinity columns. Sepharose 122-129 microtubule-associated protein 2 Rattus norvegicus 13-18 7510477-2 1994 The alpha 1-macroglobulin-proteinase complex endocytosed into rat liver lysosomes was purified by a series of column chromatographic steps on concanavalin A-Sepharose, Sephacryl S-300, DEAE-cellulose and TSK gel DEAE-5PW columns. Sepharose 157-166 pregnancy-zone protein Rattus norvegicus 4-25 8117103-6 1994 Sepharose CL-4B chromatography, glycosaminoglycan analysis and immunoprecipitation of control cell layer proteoglycans demonstrated the presence of HSPG of hydrodynamic size, Kav 0.4, resembling rat GBM HSPG in size and antigenic nature. Sepharose 0-9 syndecan 2 Rattus norvegicus 148-152 8117283-2 1994 Using Ca(2+)-dependent affinity chromatography on an isoquinolinesulfonamide derivative (CKA-1303)-coupled Sepharose, we obtained a pure form of 36-kDa MAP. Sepharose 107-116 microfibril associated protein 4 Bos taurus 145-155 8108429-6 1994 n-Sec1 binds to syntaxin 1a, 2, and 3 fusion proteins coupled to agarose beads, but not to syntaxin 4 fusion protein or beads coupled to a variety of other proteins. Sepharose 65-72 syntaxin binding protein 1 Rattus norvegicus 0-6 8174615-3 1994 Wheat germ, gorse seed and lentil lectin-agarose columns specifically retained 5-HT transporter. Sepharose 41-48 solute carrier family 6 member 4 Homo sapiens 79-95 8107809-3 1994 Here we show that both the 60K protein (p60), purified on a KLGFFKR-Sepharose affinity matrix, and recombinant calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element in a KXFFKR-sequence-specific manner. Sepharose 68-77 interferon induced protein with tetratricopeptide repeats 3 Homo sapiens 40-43 8192856-3 1994 Here we report on two putative HLA class II associated proteins (PHAPI and PHAPII) which have been purified from the cytosolic fraction of the human lymphoblastoid B-cell line H2LCL using an affinity matrix composed of the synthetic biotinylated cytoplasmic region of the DR2 alpha chain immobilized on avidin agarose. Sepharose 310-317 acidic nuclear phosphoprotein 32 family member A Homo sapiens 65-70 8192856-3 1994 Here we report on two putative HLA class II associated proteins (PHAPI and PHAPII) which have been purified from the cytosolic fraction of the human lymphoblastoid B-cell line H2LCL using an affinity matrix composed of the synthetic biotinylated cytoplasmic region of the DR2 alpha chain immobilized on avidin agarose. Sepharose 310-317 SET nuclear proto-oncogene Homo sapiens 75-81 8305421-2 1994 In this report we used immunoelectrophoresis and agarose gel electrophoresis to show increased anodic migration of the LDL particle as a result of the modification of LDL by rat CRP. Sepharose 49-56 C-reactive protein Rattus norvegicus 178-181 8305428-1 1994 In our study, 5"-nucleotidase was released from bovine liver by the treatment with Bacillus thuringiensis phosphatidylinositol-specific phospholipase C and purified to a homogeneous state by concanavalin A-Sepharose and (diethylaminoethyl)-Toyopearl column chromatography and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 206-215 5'-nucleotidase Bos taurus 14-29 8136082-1 1994 A toxic phospholipase A2 (PLA2) was isolated from Taiwan-habu-snake (Trimeresurus mucrosquamatus) venom by sequential chromatographies on CM-52, Sephadex G-75 and S-Sepharose columns. Sepharose 165-174 phospholipase A2, group V Mus musculus 26-30 7507794-8 1994 The elution patterns of the serum AFP on lectin-affinity sepharose column study also suggested a correlation with fetal gut differentiation. Sepharose 57-66 alpha fetoprotein Homo sapiens 34-37 8306501-7 1994 F(ab")2 fragments from three anti-MPO containing sera and two affinity-purified anti-MPO antibodies were eluted by affinity chromatography from Sepharose-bound PHIG F(ab")2 and showed anti-MPO antibody activity. Sepharose 144-153 myeloperoxidase Homo sapiens 85-88 8306501-7 1994 F(ab")2 fragments from three anti-MPO containing sera and two affinity-purified anti-MPO antibodies were eluted by affinity chromatography from Sepharose-bound PHIG F(ab")2 and showed anti-MPO antibody activity. Sepharose 144-153 myeloperoxidase Homo sapiens 85-88 7514789-1 1994 Thiostatin was purified from acute phase plasma of turpentine-treated rats by a novel, single-step carboxymethyl-papain Sepharose 4B column chromatographic procedure. Sepharose 120-129 kininogen 2 Rattus norvegicus 0-10 9747332-1 1994 We report the use of an allele-specific polymerase chain reaction (ASPCR) format together with low melting temperature agarose gel electrophoresis which allows rapid identification of the six major genotypes of the ABO blood group. Sepharose 119-126 ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Homo sapiens 215-230 8294456-6 1994 The in vitro product also formed a complex with eIF-4E, as judged by its ability to bind to m7GTP-Sepharose. Sepharose 98-107 eukaryotic translation initiation factor 4E Homo sapiens 48-54 7524706-4 1994 We affinity purified TSHr auto-antibodies from four Graves" patients using the three above noted peptides bound to epoxy-activated sepharose. Sepharose 131-140 thyroid stimulating hormone receptor Homo sapiens 21-25 7511169-5 1994 Sucrose density gradient fractionation provided evidence that at least some of the PrP capable of binding heparin-agarose was monomeric. Sepharose 114-121 prion protein Mus musculus 83-86 7511169-7 1994 The relative efficacies of pentosan polysulfate, Congo red, heparin, and chondroitin sulfate in blocking PrP binding to heparin-agarose corresponded with their previously demonstrated potencies in inhibiting protease-resistant PrP accumulation. Sepharose 128-135 prion protein Mus musculus 105-108 8287615-4 1994 Gelatin Sepharose chromatography and gel filtration chromatography were employed as partial purification procedures for MMP-2 and MMP-9. Sepharose 8-17 matrix metallopeptidase 2 Homo sapiens 120-125 8011429-9 1994 Chromatography of human platelet extract on heparin-Sepharose resolved two peaks of MLC phosphatase activity: PP2A in 0.1 M NaCl eluate and PP1 in 0.5 NaCl eluate. Sepharose 52-61 modulator of VRAC current 1 Homo sapiens 84-87 7803688-9 1994 The PAI-1 was purified 51 folds to homogeneity from serum free medium of HepG2 with the recovery rate of 92% by one-step procedure using Sepharose 4B conjugated with anti-PAI-1 McAb (AP1, AP3 and AP4). Sepharose 137-146 serine (or cysteine) peptidase inhibitor, clade E, member 1 Mus musculus 4-9 7496925-2 1994 The beta-2 transferrin was detected by agarose gel electrophoresis of the fluid on Beckman Paragon equipment, followed by pressure transfer to a nitrocellulose membrane and then incubation with enzyme-labeled antitransferrin antibody and substrate. Sepharose 39-46 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 4-10 7496925-2 1994 The beta-2 transferrin was detected by agarose gel electrophoresis of the fluid on Beckman Paragon equipment, followed by pressure transfer to a nitrocellulose membrane and then incubation with enzyme-labeled antitransferrin antibody and substrate. Sepharose 39-46 transferrin Homo sapiens 11-22 8054509-2 1994 Following p-aminobenzamidine- and D-phenylalanine-agarose affinity chromatography both trypsin- and chymotrypsin-like protease activities were found. Sepharose 50-57 mast cell protease 1-like 1 Rattus norvegicus 87-126 7890268-4 1994 Analysis of plasma vWf multimeric structure by short-SDS-agarose gel electrophoresis showed an abnormal banding pattern for each vWf oligomer, which was organized as a doublet instead of the normal triplet. Sepharose 57-64 von Willebrand factor Homo sapiens 19-22 7890268-4 1994 Analysis of plasma vWf multimeric structure by short-SDS-agarose gel electrophoresis showed an abnormal banding pattern for each vWf oligomer, which was organized as a doublet instead of the normal triplet. Sepharose 57-64 von Willebrand factor Homo sapiens 129-132 8199465-3 1994 Protein A-Sepharose did not bind to monoclonal IgE, but bound to 12-14% of serum polyclonal IgE and to -3% of the polyclonal IgE purified from the serum. Sepharose 10-19 immunoglobulin heavy constant epsilon Homo sapiens 92-95 7544773-8 1994 Using laminin-Sepharose affinity chromatography, more beta 1 integrin was obtained from the laminin-adherent subclone. Sepharose 14-23 integrin subunit beta 1 Homo sapiens 54-69 8199465-3 1994 Protein A-Sepharose did not bind to monoclonal IgE, but bound to 12-14% of serum polyclonal IgE and to -3% of the polyclonal IgE purified from the serum. Sepharose 10-19 immunoglobulin heavy constant epsilon Homo sapiens 92-95 9222865-5 1994 Agarose column chromatography and gradient gel electrophoresis indicated that alpha-apoE belongs to early fractionated HDL, and that the precipitatable alpha-ApoE belongs to the higher molecular size HDL alpha-ApoE (%) estimated by immunofixation showed a strong positive correlation with apoE (%) in pHDL-fr. Sepharose 0-7 apolipoprotein E Homo sapiens 84-88 7508463-9 1994 By affinity chromatography with peptide-Sepharose anti-Fel d I antibodies were isolated, also confirming the specificity of IgE binding to the peptides. Sepharose 40-49 major allergen I polypeptide chain 2 Felis catus 55-62 7508463-9 1994 By affinity chromatography with peptide-Sepharose anti-Fel d I antibodies were isolated, also confirming the specificity of IgE binding to the peptides. Sepharose 40-49 immunoglobulin heavy constant epsilon Homo sapiens 124-127 7508463-10 1994 The percentage of IgE antibodies against Fel d I reactive with the peptides varied with the serum and the peptide-Sepharose used and ranged from 2% to 55%. Sepharose 114-123 immunoglobulin heavy constant epsilon Homo sapiens 18-21 7508463-10 1994 The percentage of IgE antibodies against Fel d I reactive with the peptides varied with the serum and the peptide-Sepharose used and ranged from 2% to 55%. Sepharose 114-123 major allergen I polypeptide chain 2 Felis catus 41-48 8046546-5 1994 Agarose gels provided better separation of ALP isoenzymes than cellulose acetate gels. Sepharose 0-7 ATHS Homo sapiens 43-46 7714548-3 1994 Exposure of rat glioma cell line KEG-1 to ACNU for 2 hours resulted in oligonucleosomal DNA fragmentation, creating a "ladder" on agarose gel electrophoresis. Sepharose 130-137 glycine-N-acyltransferase-like 2 Rattus norvegicus 33-38 7530143-3 1994 This we achieved by affinity chromatography of tissue extracts and of isolated proteins over tenascin-Sepharose and by solid-phase assays using the individual proteins. Sepharose 102-111 avian tenascin X Gallus gallus 93-101 8289486-3 1994 Different size products are generated from ela2 (p190) and b3a2 or b2a2 (p210) BCR-ABL transcripts which are readily and unambiguously distinguishable after agarose gel electrophoresis without the need for either nested PCR or hybridization. Sepharose 157-164 elastase, neutrophil expressed Homo sapiens 43-47 8289486-3 1994 Different size products are generated from ela2 (p190) and b3a2 or b2a2 (p210) BCR-ABL transcripts which are readily and unambiguously distinguishable after agarose gel electrophoresis without the need for either nested PCR or hybridization. Sepharose 157-164 contactin associated protein 1 Homo sapiens 49-53 8289486-3 1994 Different size products are generated from ela2 (p190) and b3a2 or b2a2 (p210) BCR-ABL transcripts which are readily and unambiguously distinguishable after agarose gel electrophoresis without the need for either nested PCR or hybridization. Sepharose 157-164 ABL proto-oncogene 1, non-receptor tyrosine kinase Homo sapiens 79-86 7696973-5 1994 The identity of this protein as the P1A gene product was confirmed by cell-free transcription-translation of the P1A cDNA, the product of which also migrated near 40 kDa in SDS-PAGE and was captured by protein A-Sepharose in the presence of the antiserum. Sepharose 212-221 zinc finger protein 185 Mus musculus 36-39 7696973-5 1994 The identity of this protein as the P1A gene product was confirmed by cell-free transcription-translation of the P1A cDNA, the product of which also migrated near 40 kDa in SDS-PAGE and was captured by protein A-Sepharose in the presence of the antiserum. Sepharose 212-221 zinc finger protein 185 Mus musculus 113-116 7530143-8 1994 Glypican bound to a column of tenascin-Sepharose cannot be dissociated by chondroitin sulfate or dermatan sulfate, but elutes in a broad peak with a gradient of heparan sulfate and in a sharper peak with heparin. Sepharose 39-48 avian tenascin X Gallus gallus 30-38 8262246-4 1993 Furthermore, agarose gel immunoelectrophoresis of solubilized brain microsomes followed by ATPase assay directly in the gel revealed ATPase activity associated with the NCAM immunoprecipitate. Sepharose 13-20 neural cell adhesion molecule 1 Rattus norvegicus 169-173 8253764-5 1993 We characterize the RAD1/RAD10 endonuclease activity on both single-stranded and double-stranded DNAs, using agarose gel electrophoresis and trichloroacetic acid precipitation. Sepharose 109-116 ssDNA endodeoxyribonuclease RAD1 Saccharomyces cerevisiae S288C 20-24 8165633-6 1994 In addition, different fractions with low affinity for ATIII were compared as competitors of 125I-vWF binding to heparin-agarose. Sepharose 121-128 von Willebrand factor Homo sapiens 98-101 8253749-3 1993 Final purification of GM3-synthase was achieved by chromatography on a "lactosylceramide acid"-Sepharose column and specific elution with lactosylceramide. Sepharose 95-104 ST3 beta-galactoside alpha-2,3-sialyltransferase 5 Rattus norvegicus 22-34 8253764-5 1993 We characterize the RAD1/RAD10 endonuclease activity on both single-stranded and double-stranded DNAs, using agarose gel electrophoresis and trichloroacetic acid precipitation. Sepharose 109-116 DNA repair protein RAD10 Saccharomyces cerevisiae S288C 25-30 8253764-5 1993 We characterize the RAD1/RAD10 endonuclease activity on both single-stranded and double-stranded DNAs, using agarose gel electrophoresis and trichloroacetic acid precipitation. Sepharose 109-116 endonuclease Saccharomyces cerevisiae S288C 31-43 8280076-5 1993 *I-DLT-hirudin which bound to thrombin was isolated by chromatography on concanavalin A-Sepharose; hirudin itself does not bind to concanavalin A. Sepharose 88-97 coagulation factor II Rattus norvegicus 30-38 8253796-3 1993 The partially purified PACAP receptors were mixed with biotinylated PACAP27 to form receptor-ligand complexes and then adsorbed onto avidin-agarose. Sepharose 140-147 adenylate cyclase activating polypeptide 1 Bos taurus 23-28 8258717-8 1993 The possible IgA receptor (IgA-R) was purified from MC by affinity chromatography on an IgA-Sepharose column and appeared on SDS-PAGE gels as a 60-kDa band. Sepharose 92-101 CD79a molecule Homo sapiens 13-16 8258717-8 1993 The possible IgA receptor (IgA-R) was purified from MC by affinity chromatography on an IgA-Sepharose column and appeared on SDS-PAGE gels as a 60-kDa band. Sepharose 92-101 CD79a molecule Homo sapiens 27-30 8258717-8 1993 The possible IgA receptor (IgA-R) was purified from MC by affinity chromatography on an IgA-Sepharose column and appeared on SDS-PAGE gels as a 60-kDa band. Sepharose 92-101 CD79a molecule Homo sapiens 27-30 8279543-5 1993 A 116-kDa cell surface protein was metabolically labeled with [35S]methionine and bound to LPL-Sepharose. Sepharose 95-104 lipoprotein lipase Rattus norvegicus 91-94 8128455-2 1993 First, we prepared fibrinogen-coated agarose beads (fbg-beads) as a model of platelets, and subjected them to aggregometry using TSP as an inducer. Sepharose 37-44 fibrinogen beta chain Homo sapiens 19-29 8288876-3 1993 Agarose blocks containing supernatants from ConA activated rat spleen cells attracted neutrophils within 4 h. These cells were followed by lymphocytes and macrophages in 24 h. Flow cytometry analysis of lymphoid cells on day 1 revealed that 38% were Ig+ (B cell marker), 60% MAC-2,3+ and 20% Thy 1.2+ of which only a small fraction were expressing CD4 on their surface. Sepharose 0-7 lectin, galactose binding, soluble 3 Mus musculus 275-280 8288876-3 1993 Agarose blocks containing supernatants from ConA activated rat spleen cells attracted neutrophils within 4 h. These cells were followed by lymphocytes and macrophages in 24 h. Flow cytometry analysis of lymphoid cells on day 1 revealed that 38% were Ig+ (B cell marker), 60% MAC-2,3+ and 20% Thy 1.2+ of which only a small fraction were expressing CD4 on their surface. Sepharose 0-7 thymus cell antigen 1, theta Mus musculus 292-299 8288876-3 1993 Agarose blocks containing supernatants from ConA activated rat spleen cells attracted neutrophils within 4 h. These cells were followed by lymphocytes and macrophages in 24 h. Flow cytometry analysis of lymphoid cells on day 1 revealed that 38% were Ig+ (B cell marker), 60% MAC-2,3+ and 20% Thy 1.2+ of which only a small fraction were expressing CD4 on their surface. Sepharose 0-7 CD4 antigen Mus musculus 348-351 8080606-2 1993 In the normal Indian population, purification of CR1 by immunoprecipitation or C3b-Sepharose affinity column and subjecting it to electrophoresis showed the existence of two types of structural polymorphic patterns with M(r) of 190 kDa and 220 kDa, and with gene frequencies of 0.975 and 0.025, respectively. Sepharose 83-92 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 49-52 8241100-9 1993 Soft agar culture of an adenocarcinoma cell line (SW-13) demonstrates growth-stimulating activity similar to that described for pleiotrophin in the heparin-agarose eluate of postheparin plasma but not in the heparin-agarose eluate of preheparin plasma. Sepharose 156-163 pleiotrophin Homo sapiens 128-140 8080606-2 1993 In the normal Indian population, purification of CR1 by immunoprecipitation or C3b-Sepharose affinity column and subjecting it to electrophoresis showed the existence of two types of structural polymorphic patterns with M(r) of 190 kDa and 220 kDa, and with gene frequencies of 0.975 and 0.025, respectively. Sepharose 83-92 endogenous retrovirus group K member 3 Homo sapiens 79-82 8172562-7 1993 The complex formation of C1q, the most basic serum protein, with this polyanion was demonstrated by several methods: agarose gel electrophoresis followed by immunoprecipitation in the gel and Coomassie staining; western blot analysis of C1q-PRP complexes; complex formation in electrophoretic separation of PRP; retardation of electrophoretic mobility of PRP was checked by blotting of this polysaccharide. Sepharose 117-124 complement C1q A chain Homo sapiens 25-28 8136702-2 1993 MIF specifically bound glutathione (dissociation constant = 600 microM), and the protein was effectively purified by an S-hexylglutathione Sepharose affinity column. Sepharose 139-148 macrophage migration inhibitory factor Homo sapiens 0-3 8292335-2 1993 The 50-kbp fragments are further degraded, in some but not all cells, to smaller fragments (10-40 kbp) and release the small oligonucleosome fragments that are recognized as the characteristic DNA ladder on conventional agarose gels. Sepharose 220-227 kinesin family binding protein Homo sapiens 7-10 8292650-1 1993 A procedure for one-step rapid isolation of highly purified elastase and cathepsin G from human leucocytes including biospecific chromatography on Gordox-Sepharose is described. Sepharose 154-163 cathepsin G Homo sapiens 73-84 7518263-1 1993 Recombinant human acidic fibroblast growth factor (haFGF) was purified from E. coli lysate by heparin-sepharose affinity chromatography. Sepharose 102-111 fibroblast growth factor 1 Bos taurus 18-49 8312229-9 1993 Analysis of DNA after separation on agarose gels revealed that IL-10 inhibits DNA fragmentation in IL-2-starved T cells. Sepharose 36-43 interleukin 10 Homo sapiens 63-68 8194083-4 1993 For identification of the S-100-specific polypeptide, proteins of external synaptosomal membranes were iodinated with lactoperoxidase fixed on cyanogen bromide (CNBr)-Sepharose, and after synaptosome lysis S-100-positive material was isolated by means of affinity chromatography antibodies to S-100 protein (a-S-100)-Sepharose. Sepharose 317-326 S100 calcium binding protein A1 Mus musculus 26-31 8269627-3 1993 Agarose gel electrophoresis of DNA from cells treated with 10-100 microM VP-16 showed the appearance of a characteristic ladder due to the internucleosomal DNA cleavage. Sepharose 0-7 host cell factor C1 Homo sapiens 73-78 8262512-1 1993 We have developed a sensitive, high-resolutin method for the analysis of the apolipoprotein(a) [apo(a)] isoforms using sodium dodecyl sulfate (SDS)-agarose/gradient polyacrylamide gel electrophoresis. Sepharose 148-155 lipoprotein(a) Homo sapiens 77-94 8262512-1 1993 We have developed a sensitive, high-resolutin method for the analysis of the apolipoprotein(a) [apo(a)] isoforms using sodium dodecyl sulfate (SDS)-agarose/gradient polyacrylamide gel electrophoresis. Sepharose 148-155 lipoprotein(a) Homo sapiens 96-102 8312229-9 1993 Analysis of DNA after separation on agarose gels revealed that IL-10 inhibits DNA fragmentation in IL-2-starved T cells. Sepharose 36-43 interleukin 2 Homo sapiens 99-103 7510300-5 1993 8A2 IgG or FAb fragments increased the amount of alpha 5 beta 1 in cell extracts that bound to fibronectin-Sepharose and in the presence of 8A2 the amount of bound alpha 5 beta 1 in 0 hour and 5 hour extracts was equal. Sepharose 107-116 FA complementation group B Homo sapiens 11-14 8138544-8 1993 Of these three proteoglycans, only CPGIIIB proteoglycan bound specifically to fibronectin-Sepharose 4B under physiological conditions. Sepharose 90-99 fibronectin 1 Homo sapiens 78-89 8138544-10 1993 Affinity chromatographies of the CPGIIIB proteoglycan on fibronectin-Sepharose 4B after treatments with these enzymes demonstrated that it bound to fibronectin via its heparan sulfate chains. Sepharose 69-78 fibronectin 1 Homo sapiens 57-68 8138544-10 1993 Affinity chromatographies of the CPGIIIB proteoglycan on fibronectin-Sepharose 4B after treatments with these enzymes demonstrated that it bound to fibronectin via its heparan sulfate chains. Sepharose 69-78 fibronectin 1 Homo sapiens 148-159 8288243-6 1993 The mutated LPL protein was secreted from the cells in a manner similar to that of wild-type LPL and bound to heparin-Sepharose with identical properties. Sepharose 118-127 lipoprotein lipase Homo sapiens 12-15 8254099-2 1993 On agarose gel electrophoresis of the major ALP isoenzyme found in the cerebrospinal fluid, its mobility was different from those of the usual serum ALP isoenzymes. Sepharose 3-10 alkaline phosphatase, placental Homo sapiens 44-47 8263492-0 1993 Simultaneous subtyping of group specific component and esterase D by isoelectric focusing on agarose gels. Sepharose 93-100 esterase D Homo sapiens 55-65 8409443-5 1993 Flow cytometric cell cycle analysis of the JURKAT cells incubated with ISF revealed that > 90% of these cells had undergone apoptosis within 24 h. Agarose gel electrophoresis of DNA extracted from the ISF-treated lymphoid cells resolved a DNA fragmentation pattern characteristic of apoptosis in both the NSO cells and to a lesser extent in the activated PBL exposed to the ISF but not in control cells. Sepharose 150-157 ATPase, H+ transporting, lysosomal V0 subunit A2 Mus musculus 71-74 8409443-5 1993 Flow cytometric cell cycle analysis of the JURKAT cells incubated with ISF revealed that > 90% of these cells had undergone apoptosis within 24 h. Agarose gel electrophoresis of DNA extracted from the ISF-treated lymphoid cells resolved a DNA fragmentation pattern characteristic of apoptosis in both the NSO cells and to a lesser extent in the activated PBL exposed to the ISF but not in control cells. Sepharose 150-157 ATPase, H+ transporting, lysosomal V0 subunit A2 Mus musculus 204-207 8409443-5 1993 Flow cytometric cell cycle analysis of the JURKAT cells incubated with ISF revealed that > 90% of these cells had undergone apoptosis within 24 h. Agarose gel electrophoresis of DNA extracted from the ISF-treated lymphoid cells resolved a DNA fragmentation pattern characteristic of apoptosis in both the NSO cells and to a lesser extent in the activated PBL exposed to the ISF but not in control cells. Sepharose 150-157 ATPase, H+ transporting, lysosomal V0 subunit A2 Mus musculus 204-207 7874290-5 1993 Angiogenin injected in 3% agarose with low melting point into abdominal skin or into the ear skin induced neovascularization in doses no less than 3 mg. Sepharose 26-33 angiogenin Rattus norvegicus 0-10 8408007-7 1993 The cytoplasmic antithrombin activity was purified to apparent homogeneity from the cytosol of BSC-1 cells previously pulsed with [35S]methionine by a combination of heparin-agarose chromatography, Mono Q fast protein liquid chromatography, and anhydrotrypsin-Affi-Gel 10 affinity chromatography. Sepharose 174-181 serpin family C member 1 Homo sapiens 16-28 8408053-1 1993 Chondroitin 6-sulfotransferase, which transfers sulfate from 3"-phosphoadenylyl sulfate to position 6 of N-acetylgalactosamine in chondroitin, was purified 1,430-fold to apparent homogeneity with a 22% yield from the serum-free culture medium of chick embryo chondrocytes by affinity chromatography on heparin-Sepharose CL-6B, wheat germ agglutinin-agarose, and 3",5"-ADP-agarose. Sepharose 349-356 carbohydrate sulfotransferase 3 Gallus gallus 0-30 8415688-7 1993 These two phenomena were confirmed upon examination of an elution profile of VDR bound to DRE-linked Sepharose. Sepharose 101-110 vitamin D receptor Rattus norvegicus 77-80 8104708-4 1993 To address the role of this factor, BMP-4-releasing agarose beads were added to dental mesenchyme in culture. Sepharose 52-59 bone morphogenetic protein 4 Homo sapiens 36-41 8407952-2 1993 To characterize the substrate specificity of Mck1, the enzyme was purified to apparent homogeneity from the soluble fraction of yeast cell extracts by ammonium sulfate precipitation, followed by ion exchange chromatography (Q- and S-Sepharose), dye-ligand affinity chromatography (Orange A-agarose), adsorption chromatography (hydroxylapatite), and ion exchange fast protein liquid chromatography (Mono-S). Sepharose 233-242 serine/threonine/tyrosine protein kinase MCK1 Saccharomyces cerevisiae S288C 45-49 8407952-2 1993 To characterize the substrate specificity of Mck1, the enzyme was purified to apparent homogeneity from the soluble fraction of yeast cell extracts by ammonium sulfate precipitation, followed by ion exchange chromatography (Q- and S-Sepharose), dye-ligand affinity chromatography (Orange A-agarose), adsorption chromatography (hydroxylapatite), and ion exchange fast protein liquid chromatography (Mono-S). Sepharose 290-297 serine/threonine/tyrosine protein kinase MCK1 Saccharomyces cerevisiae S288C 45-49 8257702-6 1993 A series of omega-aminocarboxylic acids inhibited Lp(a), plasminogen, and K4 binding to the lysine-Sepharose beads, but marked differences in the effectiveness of these compounds were observed with each ligand. Sepharose 99-108 lipoprotein(a) Homo sapiens 50-55 8254057-5 1993 The human apo-B100 in transgenic mouse plasma was present largely in lipoproteins of the LDL class as shown by agarose gel electrophoresis, chromatography on a Superose 6 column, and density gradient ultracentrifugation. Sepharose 111-118 apolipoprotein B Homo sapiens 10-18 8246266-8 1993 Negative affinity chromatography was used to obtain antibody fractions enriched for IgG1, IgG2 or IgG4 and protein A-sepharose chromatography was used to isolate IgG3 antibodies from CF patients. Sepharose 117-126 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 162-166 8136018-9 1993 gamma T-Thrombin and thrombin Quick I (Arg 67-->Cys) are both altered in anion-binding exosite-I, yet bind to heparin-Sepharose and can be inhibited by AT, HC, and PCI in an essentially normal manner in the absence of heparin. Sepharose 121-130 coagulation factor II, thrombin Homo sapiens 8-16 8136018-9 1993 gamma T-Thrombin and thrombin Quick I (Arg 67-->Cys) are both altered in anion-binding exosite-I, yet bind to heparin-Sepharose and can be inhibited by AT, HC, and PCI in an essentially normal manner in the absence of heparin. Sepharose 121-130 coagulation factor II, thrombin Homo sapiens 21-29 8145772-5 1993 This inhibitory activity copurifies with the IR on insulin-Sepharose affinity chromatography and is also effective against the tyrosine kinase activity of the IR-related insulin-like growth factor-I receptor and the oncoprotein v-abl but is ineffective against c-src tyrosine kinase activity. Sepharose 59-68 insulin Homo sapiens 51-58 8145772-5 1993 This inhibitory activity copurifies with the IR on insulin-Sepharose affinity chromatography and is also effective against the tyrosine kinase activity of the IR-related insulin-like growth factor-I receptor and the oncoprotein v-abl but is ineffective against c-src tyrosine kinase activity. Sepharose 59-68 insulin Homo sapiens 170-177 8145772-5 1993 This inhibitory activity copurifies with the IR on insulin-Sepharose affinity chromatography and is also effective against the tyrosine kinase activity of the IR-related insulin-like growth factor-I receptor and the oncoprotein v-abl but is ineffective against c-src tyrosine kinase activity. Sepharose 59-68 ABL proto-oncogene 1, non-receptor tyrosine kinase Homo sapiens 228-233 8145772-5 1993 This inhibitory activity copurifies with the IR on insulin-Sepharose affinity chromatography and is also effective against the tyrosine kinase activity of the IR-related insulin-like growth factor-I receptor and the oncoprotein v-abl but is ineffective against c-src tyrosine kinase activity. Sepharose 59-68 C-terminal Src kinase Homo sapiens 261-282 8248203-4 1993 AT2 receptors were further purified by gel filtration and a CGP42112 Sepharose affinity column. Sepharose 69-78 angiotensin II receptor, type 2 Rattus norvegicus 0-3 8248225-4 1993 B50/GAP-43 protein was further separated into phosphorylated and dephosphorylated species by calmodulin-Sepharose chromatography. Sepharose 104-113 growth associated protein 43 Homo sapiens 0-3 8248225-4 1993 B50/GAP-43 protein was further separated into phosphorylated and dephosphorylated species by calmodulin-Sepharose chromatography. Sepharose 104-113 growth associated protein 43 Bos taurus 4-10 8023093-0 1993 Effects of ionizing radiation on the size distribution of proteoglycan aggregates synthesized by chondrocytes in agarose. Sepharose 113-120 versican Gallus gallus 58-70 8149633-2 1993 Electrophoresis on agarose gel displays a better resolving power than SDS-PAGE (a larger number of apo(a) isoforms is detected). Sepharose 19-26 lipoprotein(a) Homo sapiens 99-105 8227071-4 1993 The purification procedure included anion-exchange, cation-exchange, gel filtration, heparin-Sepharose, and chromatography on a column of thiophosphorylated MAP kinase-Sepharose, resulting in a > 3000-fold purification. Sepharose 168-177 mitogen-activated protein kinase 1 S homeolog Xenopus laevis 157-167 7504471-6 1993 cdc25C was also found associated with cdc2-p13-Sepharose complex and its PTP activity was 7-fold higher in samples from mitotic than from asynchronous cells. Sepharose 47-56 cell division cycle 25C Homo sapiens 0-6 7504471-6 1993 cdc25C was also found associated with cdc2-p13-Sepharose complex and its PTP activity was 7-fold higher in samples from mitotic than from asynchronous cells. Sepharose 47-56 cyclin dependent kinase 1 Homo sapiens 0-4 8111962-2 1993 A subfraction of HDL enriched in apolipoprotein E (apo E), separated by heparin-Sepharose affinity chromatography, was present in lower concentrations (P < 0.001) in the plasma of the coronary patients than in the control subjects. Sepharose 80-89 apolipoprotein E Homo sapiens 33-49 8111962-2 1993 A subfraction of HDL enriched in apolipoprotein E (apo E), separated by heparin-Sepharose affinity chromatography, was present in lower concentrations (P < 0.001) in the plasma of the coronary patients than in the control subjects. Sepharose 80-89 apolipoprotein E Homo sapiens 51-56 8239650-2 1993 By using murine IL-5-coupled Sepharose, the single step purification method of the protein was established. Sepharose 29-38 interleukin 5 Mus musculus 16-20 8293961-4 1993 Plasmin was detected by hydrolysis of skimmed milk protein in agarose. Sepharose 62-69 plasminogen Bos taurus 0-7 8128448-6 1993 The binding of u-PA and t-PA to heparin-agarose was less salt sensitive. Sepharose 40-47 plasminogen activator, urokinase Homo sapiens 15-19 8128448-6 1993 The binding of u-PA and t-PA to heparin-agarose was less salt sensitive. Sepharose 40-47 plasminogen activator, tissue type Homo sapiens 24-28 8226849-5 1993 Aginactin capping activity and the 70-kDa protein bind to ATP-agarose columns and are quantitatively depleted from the load, indicating that an Hsc70 is associated with aginactin activity. Sepharose 62-69 heat shock protein family A (Hsp70) member 8 Homo sapiens 144-149 8226865-14 1993 Here, we describe the synthesis of mercurated agarose magnetic beads with high capacity for SH groups and test their application to the recovery of chromatin restriction fragments of c-myc and the growth arrest gene gas1. Sepharose 46-53 MYC proto-oncogene, bHLH transcription factor Homo sapiens 183-188 8226865-14 1993 Here, we describe the synthesis of mercurated agarose magnetic beads with high capacity for SH groups and test their application to the recovery of chromatin restriction fragments of c-myc and the growth arrest gene gas1. Sepharose 46-53 growth arrest specific 1 Homo sapiens 216-220 8215459-10 1993 Furthermore, when immobilized on Sepharose in a manner which does not compromise essential cationic residues, the vWF domain peptides are effective affinity ligands. Sepharose 33-42 von Willebrand factor Homo sapiens 114-117 8118427-1 1993 A soluble protein kinase (PK) was purified from bovine and human follicular fluids (FF) by ultrafiltration through a PM-10 membrane followed by chromatography on heparin-agarose, DEAE-cellulose and cellulose phosphate columns. Sepharose 170-177 protein kinase cAMP-activated catalytic subunit beta Bos taurus 10-24 8118427-1 1993 A soluble protein kinase (PK) was purified from bovine and human follicular fluids (FF) by ultrafiltration through a PM-10 membrane followed by chromatography on heparin-agarose, DEAE-cellulose and cellulose phosphate columns. Sepharose 170-177 protein kinase cAMP-activated catalytic subunit beta Bos taurus 26-28 7693043-8 1993 Preliminary studies show that a proportion of the PECAM-1 molecules on the lymphomyeloid/multipotential progenitor cell line, KG1, and on the monocytic cell line, U937, binds to heparin-sepharose. Sepharose 186-195 platelet and endothelial cell adhesion molecule 1 Homo sapiens 50-57 7693043-9 1993 A soluble form of PECAM-1 also binds heparin-sepharose. Sepharose 45-54 platelet and endothelial cell adhesion molecule 1 Homo sapiens 18-25 8222162-5 1993 Endothelial cell fibronectin synthesis was determined after radiolabeling with [35S]-methionine in serum-free medium, gelatin-sepharose extraction of the culture medium and resolution on 5% SDS-PAGE. Sepharose 126-135 fibronectin 1 Homo sapiens 17-28 8222806-7 1993 In addition to mild loss of the largest multimers, changes in oligomeric composition of plasma vWF were observed in most patients using both agarose and polyacrylamide gel electrophoresis. Sepharose 141-148 von Willebrand factor Homo sapiens 95-98 8223431-6 1993 Both p31 and p27 bind quantitatively to heparin-Sepharose and can be displaced from the cell surface and extracellular matrix by soluble heparin. Sepharose 48-57 unconventional SNARE in the ER 1 homolog (S. cerevisiae) Mus musculus 5-8 8223431-6 1993 Both p31 and p27 bind quantitatively to heparin-Sepharose and can be displaced from the cell surface and extracellular matrix by soluble heparin. Sepharose 48-57 cyclin-dependent kinase inhibitor 1B Mus musculus 13-16 8275010-6 1993 A 67 kDa protein was isolated from the hepatic plasma membrane of CCl4 induced regenerating rat liver by affinity chromatography over laminin sepharose. Sepharose 142-151 C-C motif chemokine ligand 4 Rattus norvegicus 66-70 8291446-4 1993 This bioactive molecule was determined as bFGF by using the heparin-Sepharose affinity chromatography and western blot analysis. Sepharose 68-77 fibroblast growth factor 2 Homo sapiens 42-46 7764266-3 1993 Concanavalin A-Sepharose chromatography is available for purification of the fungal PDI, indicating that the enzyme is also glycosylated like the yeast PDI. Sepharose 15-24 prolyl 4-hydroxylase subunit beta Bos taurus 84-87 7764266-3 1993 Concanavalin A-Sepharose chromatography is available for purification of the fungal PDI, indicating that the enzyme is also glycosylated like the yeast PDI. Sepharose 15-24 prolyl 4-hydroxylase subunit beta Bos taurus 152-155 8243059-2 1993 Dog liver acid beta-galactosidase was isolated in high yield and purified to homogeneity using a series of chromatographies on Con A-Sepharose, decyl-agarose, anion-exchange HPLC and gel-filtration HPLC. Sepharose 133-142 galactosidase beta 1 Canis lupus familiaris 10-33 8299212-4 1993 The agarose gel electrophoresis pattern of the patient"s serum ALP was identical to that of common intestinal ALP from healthy adults, and only a single band of intestinal ALP was detected by immunoaffinity electrophoresis. Sepharose 4-11 alkaline phosphatase, placental Homo sapiens 63-66 8125053-0 1993 Salivary deoxyribonuclease I polymorphism separated by polyacrylamide gel-isoelectric focusing and detected by the dried agarose film overlay method. Sepharose 121-128 deoxyribonuclease 1 Homo sapiens 9-28 8408473-8 1993 Inclusion of competitive standard cDNAs of known concentration in the PCR reactions allowed quantitation of the absolute levels of the beta 1-, alpha 1-, and alpha 2 mRNAs by comparison of products on ethidium bromide-stained agarose gels. Sepharose 226-233 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 135-165 8136033-1 1993 Acetylcholinesterase from human caudate nucleus and partial thalamus was purified by using Con A-Sepharose, short-arm and long-arm ligand Sepharose affinity chromatographies. Sepharose 97-106 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 8376787-6 1993 This conclusion is based on findings that four H-2Kb-transfected clones selected on SBA-agarose beads did not expressed H-2Kb Ag but manifested increase in SBA and GS1B4 lectin binding and loss of MAA and also became sensitive to TNF lysis. Sepharose 88-95 histocompatibility 2, K1, K region Mus musculus 47-52 8136033-1 1993 Acetylcholinesterase from human caudate nucleus and partial thalamus was purified by using Con A-Sepharose, short-arm and long-arm ligand Sepharose affinity chromatographies. Sepharose 138-147 acetylcholinesterase (Cartwright blood group) Homo sapiens 0-20 8136033-6 1993 Con A-Sepharose affinity chromatography retained most of the applied AChE activity implying that the enzyme is a kind of glycoprotein. Sepharose 6-15 acetylcholinesterase (Cartwright blood group) Homo sapiens 69-73 8376410-8 1993 Both recombinant proteins were also retained on thrombin-Sepharose 4B. Sepharose 57-66 coagulation factor II, thrombin Homo sapiens 48-56 8257857-3 1993 TSA was detectable in all of 11 women in normal early pregnancy, correlated positively with serum hCG concentration, and was abolished completely by the pretreatment of serum sample with the solid-phase hCG antibody coupled with Sepharose 4B. Sepharose 229-238 chorionic gonadotropin subunit beta 5 Homo sapiens 203-206 7691055-7 1993 Both proteins bound with high affinity and specificity to IGF-1 immobilized on agarose, and each underwent phosphorylation when the agarose beads were incubated with [gamma-32P]ATP and MnCl2. Sepharose 79-86 insulin-like growth factor 1 Rattus norvegicus 58-63 7691055-7 1993 Both proteins bound with high affinity and specificity to IGF-1 immobilized on agarose, and each underwent phosphorylation when the agarose beads were incubated with [gamma-32P]ATP and MnCl2. Sepharose 132-139 insulin-like growth factor 1 Rattus norvegicus 58-63 8366104-6 1993 The calmodulin binding domain in the petunia GAD was mapped by binding truncated forms of GAD immobilized on nitrocellulose membranes to recombinant petunia 35S-calmodulin as well as to biotinylated bovine calmodulin and by binding truncated forms of GAD to calmodulin-Sepharose columns. Sepharose 269-278 calmodulin Bos taurus 4-14 8373425-7 1993 Moreover, 2C5/1D10 antibodies coupled to Protein A-sepharose beads immunoprecipitated enzymatically active granzyme B from YT cell lysates. Sepharose 51-60 granzyme B Homo sapiens 107-117 8373400-4 1993 Following expression in E. Coli and purification on Glutathione-Sepharose of either the full-length recombinant p59/HBI, or the recombinant FKBP-like domains, we demonstrate by autoradiography of [alpha 32P]-8-azido ATP and of [alpha 32P]-8-azido GTP photoaffinity labeled complexes, that an ATP (GTP) binding site is located in the domain II. Sepharose 64-73 HLA complex P5B Homo sapiens 112-115 8216357-1 1993 Aldehyde oxidase was purified about 120-fold from rat liver cytosol by sequential column chromatography using diethylaminoethyl (DEAE) cellulose, Benzamidine-Sepharose 6B and gel filtration. Sepharose 158-167 aldehyde oxidase 1 Homo sapiens 0-16 8395505-7 1993 As expected, K2P showed enhanced plasminogen activation in the presence of CNBr fragments of fibrinogen, bound to lysine-Sepharose and to a forming fibrin clot. Sepharose 121-130 keratin 76 Homo sapiens 13-16 8370183-6 1993 Preincubation of SLE sera with Protein A-Sepharose beads conjugated with antibodies against tumor necrosis factor-alpha and interferon-alpha was able to significantly reduce their ability to upregulate class I MHC expression by HUVE cells, indicating that these cytokines were responsible for the modulatory effect. Sepharose 41-50 tumor necrosis factor Homo sapiens 92-119 8358737-4 1993 An M(r) 29,000 bFGF-like form eluted from heparin-Sepharose by 0.6 M NaCl was found in extracts of both NDP and LTP cells. Sepharose 50-59 fibroblast growth factor 2 Mus musculus 15-19 8358737-4 1993 An M(r) 29,000 bFGF-like form eluted from heparin-Sepharose by 0.6 M NaCl was found in extracts of both NDP and LTP cells. Sepharose 50-59 Norrie disease (pseudoglioma) (human) Mus musculus 104-107 8358737-8 1993 The M(r) 29,000 form present in the conditioned medium of NDP cells was retained on heparin-Sepharose. Sepharose 92-101 Norrie disease (pseudoglioma) (human) Mus musculus 58-61 8396016-3 1993 Insulin and IGF-I receptors were isolated by wheat germ agglutinin-agarose chromatography and immunoprecipitation with antiphosphotyrosine antibodies (alpha PY20). Sepharose 67-74 insulin Bos taurus 0-7 8396016-3 1993 Insulin and IGF-I receptors were isolated by wheat germ agglutinin-agarose chromatography and immunoprecipitation with antiphosphotyrosine antibodies (alpha PY20). Sepharose 67-74 insulin like growth factor 1 Bos taurus 12-17 8060669-1 1993 The binding of phosphorylase kinase to calmodulin-Sepharose 4B was studied by column and batch methods. Sepharose 50-59 calmodulin Oryctolagus cuniculus 39-49 8282539-6 1993 Our results indicate that the potentiating effect of forskolin (50 microM) on TNF-mediated MCF7 cell lysis did not involve a modulation in the TNF-induced activation of the nuclear factor NF-kB but was associated with an increase in the DNA fragmenting capacity of TNF as assessed by agarose gel electrophoresis of target cell DNA. Sepharose 284-291 tumor necrosis factor Homo sapiens 78-81 8060669-2 1993 It was found that the Ca2+ dependence of the interaction strongly depended on the degree of substitution of agarose with calmodulin. Sepharose 108-115 calmodulin Oryctolagus cuniculus 121-131 8228638-5 1993 The LPL mass in preheparin plasma eluted early from heparin-Sepharose, in the position expected for inactive LPL monomers. Sepharose 60-69 lipoprotein lipase Homo sapiens 4-7 8060669-3 1993 Equilibrium adsorption isotherms (i.e., bulk ligand binding functions and lattice site binding functions) of phosphorylase kinase were measured on calmodulin-Sepharose. Sepharose 158-167 calmodulin Oryctolagus cuniculus 147-157 8228638-7 1993 The increment of mass and activity after heparin eluted later from heparin-Sepharose, in the position expected for active LPL dimers. Sepharose 75-84 lipoprotein lipase Homo sapiens 122-125 8060669-6 1993 These findings were combined to optimize the adsorption of phosphorylase kinase on calmodulin-Sepharose, for purification procedures at low Ca2+ concentrations (5-10 microM) minimizing proteolysis by calpains. Sepharose 94-103 calmodulin Oryctolagus cuniculus 83-93 7689151-9 1993 The cytoplasmic region of the TCR zeta chain was synthesized, tyrosine phosphorylated, and conjugated to Sepharose beads. Sepharose 105-114 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 30-33 8191164-4 1993 These are used to reveal, by autoradiography, the bands of vWF of various molecular weights separated by electrophoresis in agarose gels. Sepharose 124-131 von Willebrand factor Homo sapiens 59-62 8246925-2 1993 These proteins, corresponding to human Oct-1 and Oct-2A nuclear factors, were purified by ion exchange and heparin-agarose chromatography. Sepharose 115-122 POU class 2 homeobox 1 Homo sapiens 39-44 8246925-2 1993 These proteins, corresponding to human Oct-1 and Oct-2A nuclear factors, were purified by ion exchange and heparin-agarose chromatography. Sepharose 115-122 POU domain, class 2, transcription factor 2 Mus musculus 49-55 8367447-4 1993 In the present study, heparin-Sepharose chromatography was used to partially purify solubilized N1E-115 membranes to produce an enriched population of AT2 receptors. Sepharose 30-39 serine (or cysteine) peptidase inhibitor, clade B, member 9d Mus musculus 151-154 8371976-8 1993 SRF was purified by binding to Wheat Germ Agglutinin (WGA)-agarose but the active factors remained in the WGA-unbound fractions. Sepharose 59-66 serum response factor Mus musculus 0-3 7694519-1 1993 Entrapment in human alpha 2-macroglobulin (alpha 2M) of non-proteolytic enzymes was achieved with the help of trypsin covalently attached to Sepharose matrix. Sepharose 141-150 alpha-2-macroglobulin Homo sapiens 20-41 8395834-2 1993 TBP co-immunoprecipitates with T antigen when incubated with the T antigen-specific monoclonal antibody PAb419, and Protein-A agarose. Sepharose 126-133 TATA-box binding protein Homo sapiens 0-3 8347617-1 1993 Chemical modification of plasma fibronectin (pFn) or its 40-kDa collagen/gelatin binding (CGB) domain by low concentrations of chloramine T (CT), a methionine-specific oxidant, caused decreased binding affinity between pFn or the isolated CGB domain and Sepharose-immobilized denatured collagen or a Texas Red-labeled CNBr fragment CB7 from the alpha 1 chain of type I collagen. Sepharose 254-263 fibronectin 1 Homo sapiens 32-43 7694519-1 1993 Entrapment in human alpha 2-macroglobulin (alpha 2M) of non-proteolytic enzymes was achieved with the help of trypsin covalently attached to Sepharose matrix. Sepharose 141-150 alpha-2-macroglobulin Homo sapiens 43-51 8393643-1 1993 The NAD glycohydrolase (NADase) was solubilized from intact erythrocytes with bacterial phosphatidylinositol-specific phospholipase C and purified to homogeneity by affinity chromatography on Cibacron blue-agarose. Sepharose 206-213 NAD glycohydrolase Oryctolagus cuniculus 4-22 7688313-4 1993 Affinity chromatography of bovine chondrocyte membrane proteins on a collagen-Sepharose column followed by immunoprecipitation confirmed the presence of the collagen-binding alpha 2 beta 1-integrin on chondrocytes. Sepharose 78-87 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 182-188 8352754-4 1993 Purified mucin, obtained from the void volume of the Sepharose column, was characterized by SDS/PAGE, amino acid and carbohydrate analyses, sensitivity to thiol reduction, and cross-reactivity with antibody preparations to rat and human intestinal mucins on Western blots. Sepharose 53-62 solute carrier family 13 member 2 Rattus norvegicus 9-14 8397885-2 1993 beta-Lactoglobulin coupled to Sepharose 4B can be used as a specific affinity matrix to remove beta-lactoglobulin from milk without the use of buffer systems. Sepharose 30-39 beta-lactoglobulin Bos taurus 95-113 8405191-5 1993 Dnp-SG ATPase was purified from bovine lens epithelium and cortex using Dnp-SG-Sepharose 6MB affinity chromatography. Sepharose 79-88 ralA binding protein 1 Homo sapiens 0-13 8104872-6 1993 GPI (EC 5.3.1.9) and PGD (EC 1.1.1.44) phenotypes were determined by agarose gel electrophoresis of isozymes. Sepharose 69-76 glucose-6-phosphate isomerase Sus scrofa 0-3 8399492-10 1993 Colony formation in soft agarose and tumorigenicity in an orthotopic lung cancer model in nu/nu mice were dramatically reduced in H460a cells expressing antisense K-ras. Sepharose 25-32 KRAS proto-oncogene, GTPase Homo sapiens 163-168 7691164-7 1993 It was also found that not only the synthetic peptide 19-34, but also the peptides 13-28 and 19-30 inhibited the binding of antigen-binding GIF to PLA2-coupled Sepharose, while peptide 25-40 failed to do so. Sepharose 160-169 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 140-143 7691164-7 1993 It was also found that not only the synthetic peptide 19-34, but also the peptides 13-28 and 19-30 inhibited the binding of antigen-binding GIF to PLA2-coupled Sepharose, while peptide 25-40 failed to do so. Sepharose 160-169 phospholipase A2, group IB, pancreas Mus musculus 147-151 8374302-1 1993 This paper reports a novel method of affinity purification of soybean and barley beta-amylase on cyclomaltohexaose-immobilized Sepharose. Sepharose 127-136 1,4-alpha-D-glucan maltohydrolase Hordeum vulgare 81-93 8105086-3 1993 With this mutation, one MnlI recognition site is abolished and a new BsmAI site is present in the DNA sequence of the SRY gene; therefore, it is easily detected by analysis of the digestion of the amplified SRY DNA fragment on an electrophoretic agarose gel. Sepharose 246-253 sex determining region Y Homo sapiens 118-121 8105086-3 1993 With this mutation, one MnlI recognition site is abolished and a new BsmAI site is present in the DNA sequence of the SRY gene; therefore, it is easily detected by analysis of the digestion of the amplified SRY DNA fragment on an electrophoretic agarose gel. Sepharose 246-253 sex determining region Y Homo sapiens 207-210 8361955-2 1993 The growth hormone-like substance was not adsorbed on Concanavalin A-Sepharose nor DEAE-cellulose, but could be purified by gel filtration on Sephadex G-50. Sepharose 69-78 gonadotropin releasing hormone receptor Rattus norvegicus 4-18 8374300-7 1993 Once renatured, as described, eIF-4 alpha can be purified by affinity chromatography on m7GTP-Sepharose. Sepharose 94-103 eukaryotic translation initiation factor 4A2 Homo sapiens 30-41 8324749-9 1993 Morphology and agarose gel analysis of DNA showed UCSD/AML1 cells underwent apoptosis when grown with GM-CSF and TGF-beta 1 but not with M-CSF and TGF-beta 1. Sepharose 15-22 RUNX family transcription factor 1 Homo sapiens 55-59 8364895-1 1993 Human melanoma HMV-1 cells formed efficiently multicellular spheroids in media containing low concentrations (0.001-0.01%) of agarose (LCAM). Sepharose 126-133 cadherin 1 Homo sapiens 135-139 8325382-4 1993 DRP was purified from the high alkaline extract of lung membranes using heparin-agarose column chromatography followed by anti-DRP immunoaffinity column chromatography. Sepharose 80-87 utrophin Homo sapiens 0-3 8328965-1 1993 A cytosolic phospholipase A2 (PLA2) has been purified to homogeneity from the human monocytic tumour cell line THP-1 by a combination of ion-exchange, heparin-agarose and hydrophobic-interaction chromatography and non-denaturing PAGE. Sepharose 159-166 phospholipase A2 group IVA Homo sapiens 2-28 8341686-9 1993 Moreover, in vitro synthesized PAB5 protein bound to poly(A)-Sepharose with high specificity. Sepharose 61-70 poly(A)-binding protein 5 Arabidopsis thaliana 31-35 8323285-1 1993 The specific interaction between fibronectin and collagen has permitted the isolation of fibronectin from plasma using gelatin-Sepharose affinity matrices. Sepharose 127-136 fibronectin 1 Homo sapiens 33-44 8323285-1 1993 The specific interaction between fibronectin and collagen has permitted the isolation of fibronectin from plasma using gelatin-Sepharose affinity matrices. Sepharose 127-136 fibronectin 1 Homo sapiens 89-100 8323285-4 1993 (ii) This tightly bound fibronectin could not be eluted from gelatin-Sepharose matrices with strong denaturing agents, such as 8.0 M urea or 6.0 M guanidium chloride. Sepharose 69-78 fibronectin 1 Homo sapiens 24-35 8323285-6 1993 (iv) Two fibronectin-derived fragments, CB52kDa and T55 kDa, selectively bound to fresh gelatin-Sepharose but not to gelatin-Sepharose previously employed to purify fibronectin, suggesting that these fragments recognize only the high affinity binding sites in gelatin. Sepharose 96-105 fibronectin 1 Homo sapiens 9-20 8328965-1 1993 A cytosolic phospholipase A2 (PLA2) has been purified to homogeneity from the human monocytic tumour cell line THP-1 by a combination of ion-exchange, heparin-agarose and hydrophobic-interaction chromatography and non-denaturing PAGE. Sepharose 159-166 phospholipase A2 group IIA Homo sapiens 30-34 8401393-3 1993 The recombinant adrenodoxin reductase was purified from the transformed E. coli cell lysates using adrenodoxin-Sepharose affinity chromatography [T. Sugiyama and T. Yamano, FEBS Lett., 52, 145 (1975)] with a yield of 2.5 mg/l of culture. Sepharose 111-120 ferredoxin reductase Bos taurus 16-37 8401310-1 1993 A 66 kDa GTP-binding protein, Gir, and insulin receptor (IR) were copurified from human placental membrane by DEAE-Sephacel and Wheat Germ Agglutinin (WGA)-Sepharose affinity chromatography. Sepharose 156-165 insulin receptor Homo sapiens 57-59 7691449-2 1993 Products of amplification of CFTR gene were analyzed in NuSieve agarose gel. Sepharose 64-71 CF transmembrane conductance regulator Homo sapiens 29-33 8407881-6 1993 We also found that HMW-hCG, but not acetone-treated HMW-hCG, bound to ATP-agarose resin. Sepharose 74-81 cilia and flagella associated protein 97 Homo sapiens 19-22 8407881-6 1993 We also found that HMW-hCG, but not acetone-treated HMW-hCG, bound to ATP-agarose resin. Sepharose 74-81 chorionic gonadotropin subunit beta 5 Homo sapiens 23-26 8325944-5 1993 The Tg mRNA from normal and goitrous thyroid tissue was first reverse transcribed and divided into five overlapping portions from positions 57-8448, and the resulting cDNAs were amplified by polymerase chain reaction and analyzed by agarose gel electrophoresis. Sepharose 233-240 thyroglobulin Homo sapiens 4-6 7688075-3 1993 The selective removal of C-reactive protein from the plasma with phosphorylethanolamine-agarose inactivated hemolysis. Sepharose 88-95 C-reactive protein Homo sapiens 25-43 8391001-15 1993 In fact, the extracellular portion of CD43 bound to HSA-Sepharose, but not to ovalbumin- or glycylglycine-Sepharose. Sepharose 56-65 sialophorin Homo sapiens 38-42 8393080-7 1993 In contrast, expression of EBNA1 to -6 and LMP1 was accompanied by a shift towards a more lymphoblastoid cell line-like phenotype and by loss of agarose clonability in the E95B convertant. Sepharose 145-152 PDZ and LIM domain 7 Homo sapiens 43-47 8514746-6 1993 TBP co-immunoprecipitates with wild-type or mutant human p53 when incubated with the p53-specific monoclonal antibody and Protein A-agarose. Sepharose 132-139 TATA-box binding protein Homo sapiens 0-3 8499486-8 1993 Several criteria indicate that this enzyme is a member of the family of matrix metalloproteinases: (1) this activity was inhibited by EDTA, 1,10-phenanthroline and TIMP; (2) this activity bound to a gelatin-agarose affinity resin; (3) it has a mass of approx. Sepharose 207-214 TIMP metallopeptidase inhibitor 1 Homo sapiens 164-168 7687250-3 1993 The purification scheme was optimized for high-yield production of homogeneous p66/p51 RT using a combination of chromatographic matrices in the following order: Q-Sepharose, heparin-Sepharose, phenyl-Sepharose, S-Sepharose, Poly(A)-Sepharose and Q-Sepharose. Sepharose 164-173 DNA polymerase delta 3, accessory subunit Homo sapiens 79-82 7687250-5 1993 A high yield of p66/p51 RT was obtained when the time from application to elution of heparin-Sepharose in the second chromatographic step was prolonged. Sepharose 93-102 DNA polymerase delta 3, accessory subunit Homo sapiens 16-19 7687250-5 1993 A high yield of p66/p51 RT was obtained when the time from application to elution of heparin-Sepharose in the second chromatographic step was prolonged. Sepharose 93-102 tumor protein p63 Homo sapiens 20-23 7687250-7 1993 The chromatography on S-Sepharose resolved the major heterodimeric form, p66/p51, from other heterodimeric variants. Sepharose 24-33 DNA polymerase delta 3, accessory subunit Homo sapiens 73-76 7687250-7 1993 The chromatography on S-Sepharose resolved the major heterodimeric form, p66/p51, from other heterodimeric variants. Sepharose 24-33 tumor protein p63 Homo sapiens 77-80 8364506-4 1993 Caldesmon bound to a PSK-Sepharose 4B column at low ionic strength was released at about 400 mM NaCl, whereas G-actin was not retained by the column. Sepharose 25-34 caldesmon 1 Gallus gallus 0-9 8352823-4 1993 Isolation and cross-linking of suitable fragments of fibronectin (relative molecular weights 65 and 52 kDa) to polylysine is followed by conjugation to gold thiomalate on a solid phase, gelatin-agarose affinity absorbent. Sepharose 194-201 fibronectin 1 Homo sapiens 53-64 8364506-7 1993 Fragments of 80 and 38 kDa, major products produced by chymotrypsin, bound individually to a PSK-Sepharose column, indicating that caldesmon has at least two binding sites for PSK. Sepharose 97-106 caldesmon 1 Gallus gallus 131-140 8343992-8 1993 MNTFP-Sepharose affinity chromatography in combination with preparative SDS-PAGE therefore holds promise as a method for obtaining microgram quantities of NTE for chemical analysis and sequencing. Sepharose 6-15 patatin like phospholipase domain containing 6 Gallus gallus 155-158 8504760-7 1993 PRL BP was purified from estradiol-treated ovariectomized females by an oPRL sepharose 4B affinity column. Sepharose 77-89 prolactin Rattus norvegicus 0-3 8347196-7 1993 This receptor was enriched by affinity chromatography using polymerized galaptin-Sepharose. Sepharose 81-90 galectin 1 Homo sapiens 72-80 8390354-3 1993 It could be adsorbed onto calmodulin-Sepharose and eluted in Ca(2+)-free medium as a 48-kDa protein. Sepharose 37-46 calmodulin 1 Rattus norvegicus 26-36 8370668-2 1993 When human plasma was applied to an alpha-elastin-Sepharose column at 4 degrees C, the column-binding fraction contained fibronectin. Sepharose 50-59 elastin Homo sapiens 42-49 8370597-1 1993 By covalent binding of recombinant interleukin-2 (rIL-2) to Sepharose, it was possible to immunopurify specific human anti-IL-2 antibodies from a pool of immunoglobulins obtained from healthy subjects. Sepharose 60-69 interleukin 2 Homo sapiens 35-48 8370597-1 1993 By covalent binding of recombinant interleukin-2 (rIL-2) to Sepharose, it was possible to immunopurify specific human anti-IL-2 antibodies from a pool of immunoglobulins obtained from healthy subjects. Sepharose 60-69 interleukin 2 Rattus norvegicus 50-55 8370597-1 1993 By covalent binding of recombinant interleukin-2 (rIL-2) to Sepharose, it was possible to immunopurify specific human anti-IL-2 antibodies from a pool of immunoglobulins obtained from healthy subjects. Sepharose 60-69 interleukin 2 Homo sapiens 51-55 8370668-2 1993 When human plasma was applied to an alpha-elastin-Sepharose column at 4 degrees C, the column-binding fraction contained fibronectin. Sepharose 50-59 fibronectin 1 Homo sapiens 121-132 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 fibronectin 1 Homo sapiens 40-51 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 elastin Homo sapiens 65-72 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 elastin Homo sapiens 147-154 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 fibronectin 1 Homo sapiens 162-173 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 elastin Homo sapiens 147-154 8501121-5 1993 Type I collagen carrying these substitutions bound weakly to fibronectin-sepharose and could be eluted off with 1 M urea. Sepharose 73-82 fibronectin 1 Homo sapiens 61-72 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 fibronectin 1 Homo sapiens 162-173 8370668-4 1993 However, the binding affinity of plasma fibronectin to the alpha-elastin-Sepharose column was much weaker at 25 degrees C than at 4 degrees C. The elastin-plasma fibronectin interaction was further confirmed by demonstrating the binding of alpha-elastin to fibronectin on polyvinylidene difluoride membranes using an alpha-elastin specific antibody. Sepharose 73-82 elastin Homo sapiens 147-154 8389032-7 1993 Clonability in soft agarose increased with increasing LMP1 expression. Sepharose 20-27 PDZ and LIM domain 7 Homo sapiens 54-58 8496603-4 1993 Recombinant guinea pig MCP-1 was expressed in COS-7 cells, then purified by a three step procedure with orange A-agarose, carboxymethyl-HPLC, and reversed phase-HPLC. Sepharose 113-120 chemokine (C-C motif) ligand 2 Mus musculus 23-28 8496151-3 1993 PC cell-derived growth factor (PCDGF) was purified to homogeneity from PC cell-conditioned medium as an apparent 88-kDa protein by chromatography on heparin-Sepharose, Sephacryl S-200, and phenyl-Sepharose. Sepharose 157-166 granulin Mus musculus 0-29 8496151-3 1993 PC cell-derived growth factor (PCDGF) was purified to homogeneity from PC cell-conditioned medium as an apparent 88-kDa protein by chromatography on heparin-Sepharose, Sephacryl S-200, and phenyl-Sepharose. Sepharose 157-166 granulin Mus musculus 31-36 8486673-9 1993 Rat CRP acquired the ability to bind to phosphorylcholine-Sepharose and to form the dimeric and oligomeric species prior to acquiring resistance to endo H. Studies using tunicamycin revealed that the N-linked oligosaccharide present in rat CRP was not required for formation of its dimeric component, oligomerization, ability to bind to phosphorylcholine, or secretion. Sepharose 58-67 C-reactive protein Rattus norvegicus 4-7 8512595-10 1993 Analysis of DNA by agarose gel electrophoresis showed that in GC3/c1 cells nucleosomal ladders were formed only when proliferating cells were exposed to ISCU. Sepharose 19-26 ankyrin repeat domain 36 Mus musculus 62-65 8512595-10 1993 Analysis of DNA by agarose gel electrophoresis showed that in GC3/c1 cells nucleosomal ladders were formed only when proliferating cells were exposed to ISCU. Sepharose 19-26 iron-sulfur cluster assembly enzyme Mus musculus 153-157 8503856-3 1993 After 10 min pulse-labelling, LPL protein was eluted as two peaks from heparin-agarose: peak 1 at about 0.65 M NaCl, peak 2 at about 0.95 M NaCl. Sepharose 79-86 lipoprotein lipase Cavia porcellus 30-33 7683664-6 1993 When mixtures of plasmin and STAR were adsorbed to lysine-Sepharose, STAR adsorbed quantitatively (96 +/- 1%) to the gel, whereas it was nearly quantitatively recovered in the unbound fraction (92 +/- 4%) after addition of alpha 2-antiplasmin to the mixture. Sepharose 58-67 plasminogen Homo sapiens 17-24 7683664-6 1993 When mixtures of plasmin and STAR were adsorbed to lysine-Sepharose, STAR adsorbed quantitatively (96 +/- 1%) to the gel, whereas it was nearly quantitatively recovered in the unbound fraction (92 +/- 4%) after addition of alpha 2-antiplasmin to the mixture. Sepharose 58-67 steroidogenic acute regulatory protein Homo sapiens 29-33 7683664-6 1993 When mixtures of plasmin and STAR were adsorbed to lysine-Sepharose, STAR adsorbed quantitatively (96 +/- 1%) to the gel, whereas it was nearly quantitatively recovered in the unbound fraction (92 +/- 4%) after addition of alpha 2-antiplasmin to the mixture. Sepharose 58-67 steroidogenic acute regulatory protein Homo sapiens 69-73 7683664-7 1993 Scatchard analysis of the binding of STAR to plasmin-Sepharose yielded a dissociation constant of 55 nM, whereas no specific binding of STAR to plasmin-alpha 2-antiplasmin-Sepharose could be demonstrated. Sepharose 53-62 steroidogenic acute regulatory protein Homo sapiens 37-41 7683664-7 1993 Scatchard analysis of the binding of STAR to plasmin-Sepharose yielded a dissociation constant of 55 nM, whereas no specific binding of STAR to plasmin-alpha 2-antiplasmin-Sepharose could be demonstrated. Sepharose 53-62 plasminogen Homo sapiens 45-52 7684610-3 1993 PTPase activity of agarose conjugated PTP-1B was demonstrated using the ELISA system. Sepharose 19-26 protein tyrosine phosphatase non-receptor type 1 Homo sapiens 38-44 8334107-8 1993 These findings indicate that the polysaccharide sample purified by the affinity chromatography of butanol extract of OK-432 on CNBr-activated Sepharose 4B-bound TS-2 MAb carries the IFN-gamma-inducing activity of OK-432 and marked antitumor activity. Sepharose 142-151 interferon gamma Homo sapiens 182-191 8486666-10 1993 The yield of VLA-5 fibronectin receptor bound to FN80-Sepharose columns was strongly increased upon treatment of U-937 cell lysates with mAb TS2/16. Sepharose 54-63 integrin subunit alpha 5 Homo sapiens 13-18 8486666-10 1993 The yield of VLA-5 fibronectin receptor bound to FN80-Sepharose columns was strongly increased upon treatment of U-937 cell lysates with mAb TS2/16. Sepharose 54-63 fibronectin 1 Homo sapiens 19-30 8486666-13 1993 Similarly, the yield of VLA-2 retained on a collagen I-Sepharose column was dramatically increased by pretreatment of A375 melanoma cell lysates with the mAb TS2/16. Sepharose 55-64 integrin subunit alpha 2 Homo sapiens 24-29 7506041-5 1993 The U138 PAI-2-like protein was adherent to an anti-PAI-2 immunoaffinity column and was demonstrated to be nonadherent to concanavalin A-agarose, heparin-Sepharose, and the anti-PAI-1 immunoaffinity column. Sepharose 137-144 serpin family B member 2 Homo sapiens 9-14 7506041-5 1993 The U138 PAI-2-like protein was adherent to an anti-PAI-2 immunoaffinity column and was demonstrated to be nonadherent to concanavalin A-agarose, heparin-Sepharose, and the anti-PAI-1 immunoaffinity column. Sepharose 154-163 serpin family E member 1 Homo sapiens 9-12 7506041-5 1993 The U138 PAI-2-like protein was adherent to an anti-PAI-2 immunoaffinity column and was demonstrated to be nonadherent to concanavalin A-agarose, heparin-Sepharose, and the anti-PAI-1 immunoaffinity column. Sepharose 154-163 serpin family B member 2 Homo sapiens 9-14 7506041-8 1993 A third PAI was detected that was nonadherent to concanavalin A-agarose and both of the anti-PAI columns. Sepharose 64-71 serpin family E member 1 Homo sapiens 8-11 7506041-9 1993 This 50-kDa PAI was adherent to heparin-Sepharose and thrombin-agarose columns, and was not reactive with any antibodies for either PAI-1 or PAI-2. Sepharose 40-49 serpin family E member 1 Homo sapiens 12-15 7506041-9 1993 This 50-kDa PAI was adherent to heparin-Sepharose and thrombin-agarose columns, and was not reactive with any antibodies for either PAI-1 or PAI-2. Sepharose 63-70 serpin family E member 1 Homo sapiens 12-15 8097673-2 1993 We purified GST from human placenta using drug affinity chromatography on a column of W-77 coupled with Sepharose 6B and demonstrated that W-77 bound to GST. Sepharose 104-113 glutathione S-transferase kappa 1 Homo sapiens 12-15 8143085-2 1993 The Xenopus antigen purified with a mAbH6-Sepharose column was a complex of 88 kDa and 72 kDa proteins. Sepharose 42-51 alkB homolog 6 Mus musculus 36-41 8509711-2 1993 Both apolipoprotein (apo) E-poor and apoE-rich Type IV VLDL subfractions, isolated by heparin-Sepharose chromatography, were capable of enhancing cellular cholesterol and triglyceride content. Sepharose 94-103 apolipoprotein E Mus musculus 37-41 8386317-7 1993 The phospho-CREB (P-CREB) phosphatase activity present in nuclear extracts coelutes with protein Ser/Thr phosphatase type 2A (PP2A) on Mono Q, amino-hexyl Sepharose, and heparin agarose columns and was chromatographically resolved from nuclear protein Ser/Thr-phosphatase type 1 (PP1). Sepharose 155-164 cAMP responsive element binding protein 1 Homo sapiens 12-16 8386317-7 1993 The phospho-CREB (P-CREB) phosphatase activity present in nuclear extracts coelutes with protein Ser/Thr phosphatase type 2A (PP2A) on Mono Q, amino-hexyl Sepharose, and heparin agarose columns and was chromatographically resolved from nuclear protein Ser/Thr-phosphatase type 1 (PP1). Sepharose 155-164 cAMP responsive element binding protein 1 Homo sapiens 20-24 8386317-7 1993 The phospho-CREB (P-CREB) phosphatase activity present in nuclear extracts coelutes with protein Ser/Thr phosphatase type 2A (PP2A) on Mono Q, amino-hexyl Sepharose, and heparin agarose columns and was chromatographically resolved from nuclear protein Ser/Thr-phosphatase type 1 (PP1). Sepharose 155-164 protein phosphatase 2 phosphatase activator Homo sapiens 126-130 8386317-7 1993 The phospho-CREB (P-CREB) phosphatase activity present in nuclear extracts coelutes with protein Ser/Thr phosphatase type 2A (PP2A) on Mono Q, amino-hexyl Sepharose, and heparin agarose columns and was chromatographically resolved from nuclear protein Ser/Thr-phosphatase type 1 (PP1). Sepharose 178-185 cAMP responsive element binding protein 1 Homo sapiens 12-16 8386317-7 1993 The phospho-CREB (P-CREB) phosphatase activity present in nuclear extracts coelutes with protein Ser/Thr phosphatase type 2A (PP2A) on Mono Q, amino-hexyl Sepharose, and heparin agarose columns and was chromatographically resolved from nuclear protein Ser/Thr-phosphatase type 1 (PP1). Sepharose 178-185 cAMP responsive element binding protein 1 Homo sapiens 20-24 8386317-7 1993 The phospho-CREB (P-CREB) phosphatase activity present in nuclear extracts coelutes with protein Ser/Thr phosphatase type 2A (PP2A) on Mono Q, amino-hexyl Sepharose, and heparin agarose columns and was chromatographically resolved from nuclear protein Ser/Thr-phosphatase type 1 (PP1). Sepharose 178-185 protein phosphatase 2 phosphatase activator Homo sapiens 126-130 8481396-5 1993 Binding studies with L-2 LOX bound to Sepharose indicated BC-binding and inhibition with the immobilized LOX. Sepharose 38-47 linoleate 9S-lipoxygenase-4 Glycine max 25-28 8481396-5 1993 Binding studies with L-2 LOX bound to Sepharose indicated BC-binding and inhibition with the immobilized LOX. Sepharose 38-47 linoleate 9S-lipoxygenase-4 Glycine max 105-108 7686248-3 1993 We have described a serine kinase (here designated insulin receptor serine (IRS) kinase) from rat liver membranes that co-purifies with IR on wheat germ agglutinin-agarose. Sepharose 164-171 insulin receptor Rattus norvegicus 51-67 7686248-3 1993 We have described a serine kinase (here designated insulin receptor serine (IRS) kinase) from rat liver membranes that co-purifies with IR on wheat germ agglutinin-agarose. Sepharose 164-171 insulin receptor Rattus norvegicus 76-78 8496277-3 1993 The 17 beta-HSD-NADP+ complex was prepared using NADP+ elution in a Blue-Sepharose column. Sepharose 73-82 hydroxysteroid 17-beta dehydrogenase 1 Homo sapiens 4-15 8463302-2 1993 RNase L was purified from human Daudi cells on a column of 2-5A-Sepharose and used to immunize Balb/c mice. Sepharose 64-73 ribonuclease L Homo sapiens 0-7 8458437-0 1993 Assignment of the ligand binding site of the porcine estradiol receptor to the N-terminal 17 kDa part of domain E. Ligand-filled porcine estradiol receptor was adsorbed to heparin-Sepharose, from which a 26 kDa fragment was released by papain. Sepharose 180-189 estrogen receptor 1 Homo sapiens 53-71 8458437-0 1993 Assignment of the ligand binding site of the porcine estradiol receptor to the N-terminal 17 kDa part of domain E. Ligand-filled porcine estradiol receptor was adsorbed to heparin-Sepharose, from which a 26 kDa fragment was released by papain. Sepharose 180-189 estrogen receptor 1 Homo sapiens 137-155 8463263-3 1993 The CEs in both the cytosolic and the vacuolar fractions were highly purified by a simple method involving Percoll density gradient fractionation, chromatography on concanavalin A-Sepharose, Mono Q, and TSK-GelG2000SW, and termed s-CE and v-CE, respectively. Sepharose 180-189 cathepsin E Homo sapiens 4-6 8470887-5 1993 In contrast, binding of thrombin to p-CBA agarose was eliminated by modification of the active site histidine using either H-D-phenylalanyl-L-prolyl-L-arginine chloromethylketone or dansyl-L-glutamyl-glycyl-L-arginine chloromethylketone but not with tosyl-L-lysine chloromethylketone. Sepharose 42-49 coagulation factor II, thrombin Homo sapiens 24-32 8470887-7 1993 These results indicate that p-CBA agarose binds to thrombin outside of the enzyme active site and its use should be valuable in characterizing site-specific modified thrombins obtained by either protein engineering or chemical modification. Sepharose 34-41 coagulation factor II, thrombin Homo sapiens 51-59 8349731-5 1993 In addition, laminin-Sepharose quantitatively precipitated purified dystrophin-glycoprotein complex, demonstrating that the laminin-binding site is accessible when dystroglycan is associated with the complex. Sepharose 21-30 dystrophin Homo sapiens 68-78 8479904-5 1993 MEP-1 does not appear to be glycosylated since it eluted with the flow-through on a Wheat Germ Sepharose column. Sepharose 95-104 meprin 1 alpha Mus musculus 0-5 8463260-5 1993 Amino-terminal deletions in BiP eliminated ATP-agarose binding. Sepharose 47-54 heat shock protein family A (Hsp70) member 5 Homo sapiens 28-31 8489015-5 1993 We provide methods for the purification of GST fusion proteins at analytical and preparative scales, and demonstrate that saturation of glutathione agarose is dependent on fusion protein molecular weight. Sepharose 148-155 glutathione S-transferase kappa 1 Homo sapiens 43-46 8484904-3 1993 bFGF purification comprises three steps: extraction and chromatographies on S-Sepharose and heparin-Sepharose. Sepharose 78-87 fibroblast growth factor 2 Homo sapiens 0-4 8484904-3 1993 bFGF purification comprises three steps: extraction and chromatographies on S-Sepharose and heparin-Sepharose. Sepharose 100-109 fibroblast growth factor 2 Homo sapiens 0-4 8484904-5 1993 PALP purification comprises four steps: acidic butan-1-ol extraction and chromatographies on Q-Sepharose, concanavalin A-Sepharose and Q-Sepharose. Sepharose 95-104 alkaline phosphatase, placental Homo sapiens 0-4 8508141-1 1993 Glutathione S-transferase (GST) isoenzymes of rat liver cytosol were purified from a S-hexylglutathione Sepharose affinity column without using S-hexyl-glutathione for elution. Sepharose 104-113 hematopoietic prostaglandin D synthase Rattus norvegicus 0-25 8508141-1 1993 Glutathione S-transferase (GST) isoenzymes of rat liver cytosol were purified from a S-hexylglutathione Sepharose affinity column without using S-hexyl-glutathione for elution. Sepharose 104-113 hematopoietic prostaglandin D synthase Rattus norvegicus 27-30 7680955-2 1993 The immunogenicity of the concanavalin A-Sepharose-binding protein fraction of Meth A cytosol had been attributed to gp96, the major component of this fraction. Sepharose 41-50 heat shock protein 90, beta (Grp94), member 1 Mus musculus 117-121 8485105-2 1993 We show that at 70 degrees C in the sol state agarose chains are fairly rigid with a lower limiting value for the persistence length of about 9 nm and a mass per unit length of muL = 360 +/- 36 g/mol x nm. Sepharose 46-53 tripartite motif containing 37 Homo sapiens 177-180 8494994-3 1993 The patient"s vWF-multimeric structure on SDS agarose gel electrophoresis was similar to that in normal subjects. Sepharose 46-53 von Willebrand factor Homo sapiens 14-17 8458861-8 1993 Nup155 is among the integral of 30 proteins that were extracted from rat liver nuclear envelopes by 2.0 M urea/1.0 mM EDTA, separated from WGA-reactive proteins by WGA-Sepharose and further subfractionated by SDS-hydroxylapatite. Sepharose 168-177 nucleoporin 155 Rattus norvegicus 0-6 7683331-5 1993 Anti-MBP was isolated from purified IgG from brain and spinal cord by MBP-Sepharose affinity chromatography. Sepharose 74-83 myelin basic protein Homo sapiens 5-8 8486673-10 1993 The non-glycosylated rat CRP, however, was still able to bind to phosphorylcholine-Sepharose and to be secreted by hepatocytes. Sepharose 83-92 C-reactive protein Rattus norvegicus 25-28 7681142-5 1993 SNT was phosphorylated in vitro on serine, threonine, and tyrosine in p13suc1-agarose precipitates from NGF-treated PC12 cells, indicating that this protein may be a substrate of kinase activities associated with p13suc1-p34cdc2/cdk2 complexes. Sepharose 78-85 nerve growth factor Rattus norvegicus 104-107 8496672-6 1993 Before use in the CETP-IRMA, the antibodies were affinity-purified by chromatography on CETP-Sepharose. Sepharose 93-102 cholesteryl ester transfer protein Homo sapiens 88-92 8449919-9 1993 Both the sucrose gradient experiments and m7GTP-Sepharose 4B assays demonstrated that the [14C]p46 subunit of [14C]eIF-4F was displaced in the presence of eIF-4AI or eIF-4AII and that mouse [14C]eIF-4AI or [14C]eIF-4AII became incorporated into the eIF-4F complex in the same manner as rabbit reticulocyte eIF-4A. Sepharose 48-57 eukaryotic translation initiation factor 4A1 Mus musculus 155-162 7681142-1 1993 To elucidate the signal transduction mechanisms used by ligands that induce differentiation and the cessation of cell division, we utilized p13suc1-agarose, a reagent that binds p34cdc2/cdk2. Sepharose 148-155 cyclin dependent kinase 2 Rattus norvegicus 186-190 8464426-3 1993 The availability of human and rat C1q allowed comparison of the two molecules, revealing differences in their mobility on SDS-PAGE as well as on agarose gel electrophoresis. Sepharose 145-152 complement C1q A chain Homo sapiens 34-37 8469911-2 1993 Glycation of LDL at a molar ratio of 4 mol glucose mol-1 apoB, decreases affinity for heparin, as shown by heparin-agarose affinity chromatography since salt molarity needed for elution decreases from 550 mmol l-1 for control LDL (c-LDL) to 350 mmol l-1 for glycated LDL (glc-LDL). Sepharose 115-122 apolipoprotein B Homo sapiens 57-61 8469927-1 1993 Serum amyloid A (SAA) protein, a sensitive acute phase protein and the precursor of protein AA in secondary amyloid, was purified from pooled acute phase rabbit serum using two different methods: isolation of protein SAA directly by octyl-Sepharose chromatography of total serum, and dissociation and isolation of apoSAA from acute phase high density lipoprotein (HDL). Sepharose 238-248 serum amyloid A-3 protein Oryctolagus cuniculus 0-15 8506615-4 1993 When lattices were sensitized under the same condition with the IgG antibodies of different avidity levels separated from the above-mentioned antisera using Protein A and canine CRP-Sepharose 4B immunosorbent, these demonstrated different patterns of agglutinability in slide RPLA. Sepharose 182-194 C-reactive protein Canis lupus familiaris 178-181 8449919-2 1993 Using sucrose gradient centrifugation and an m7GTP-Sepharose 4B assay, it was shown that eIF-4A exchanges with the p46 subunit of eIF-4F. Sepharose 51-60 eukaryotic initiation factor 4A-I Oryctolagus cuniculus 89-95 8364464-5 1993 The effect disappeared by the treatment of NMS with gelatin-Sepharose which removed fibronectin (FN) from the serum, suggesting a significant contribution of FN on GIZUP. Sepharose 60-69 fibronectin 1 Mus musculus 84-95 8364464-5 1993 The effect disappeared by the treatment of NMS with gelatin-Sepharose which removed fibronectin (FN) from the serum, suggesting a significant contribution of FN on GIZUP. Sepharose 60-69 fibronectin 1 Mus musculus 97-99 8452539-5 1993 Recombinant bovine intestinal alkaline phosphatase displays enzymatic properties comparable with those of purified native bovine intestinal alkaline phosphatase, a slightly increased thermal stability and, upon desialylation, it shows a homogeneous behaviour in agarose gel electrophoresis and isoelectric focusing. Sepharose 262-269 alkaline phosphatase 3, intestine, not Mn requiring Mus musculus 19-50 8448855-1 1993 In the single radial enzyme-diffusion (SRED) method for assay of deoxyribonuclease I, a precisely measured volume of the enzyme solution is dispensed into a circular well in an agarose gel layer in which DNA and ethidium bromide are uniformly distributed. Sepharose 177-184 deoxyribonuclease 1 Homo sapiens 65-84 8364981-4 1993 The partially purified growth factor from the NPC appeared to be related to the HGF isolated from platelets according to three criteria: (a) binding to Heparin-Sepharose and eluting at about 0.65 M NaCl, (b) having a Mr of about 70 kDa, (c) having an immunoreactivity to antibody against rat platelet-derived HGF. Sepharose 160-169 myotrophin Rattus norvegicus 23-36 8364981-4 1993 The partially purified growth factor from the NPC appeared to be related to the HGF isolated from platelets according to three criteria: (a) binding to Heparin-Sepharose and eluting at about 0.65 M NaCl, (b) having a Mr of about 70 kDa, (c) having an immunoreactivity to antibody against rat platelet-derived HGF. Sepharose 160-169 hepatocyte growth factor Rattus norvegicus 80-83 8449960-7 1993 The GST-hDRS fusion protein and the GST-hDRS delta 32 were purified by affinity chromatography on glutathione-agarose and were fully active in aspartylation of mammalian tRNA. Sepharose 110-117 sushi repeat containing protein X-linked Homo sapiens 8-12 8449960-7 1993 The GST-hDRS fusion protein and the GST-hDRS delta 32 were purified by affinity chromatography on glutathione-agarose and were fully active in aspartylation of mammalian tRNA. Sepharose 110-117 sushi repeat containing protein X-linked Homo sapiens 40-44 8449960-8 1993 After cleavage of GST from the fusion proteins by thrombin, hDRS and hDRS delta 32 were purified by affinity chromatography on tRNA-Sepharose. Sepharose 132-141 sushi repeat containing protein X-linked Homo sapiens 60-64 8449960-8 1993 After cleavage of GST from the fusion proteins by thrombin, hDRS and hDRS delta 32 were purified by affinity chromatography on tRNA-Sepharose. Sepharose 132-141 sushi repeat containing protein X-linked Homo sapiens 69-73 8444841-2 1993 The resulting pools of di-, tetra-, hexa-, octa-, and decasaccharides were sequentially applied to an affinity column of human recombinant basic fibroblast growth factor (bFGF) covalently attached to Sepharose 4B and further fractionated into subpools based on their elution from this column in response to gradients of sodium chloride. Sepharose 200-212 fibroblast growth factor 2 Homo sapiens 139-169 8444841-2 1993 The resulting pools of di-, tetra-, hexa-, octa-, and decasaccharides were sequentially applied to an affinity column of human recombinant basic fibroblast growth factor (bFGF) covalently attached to Sepharose 4B and further fractionated into subpools based on their elution from this column in response to gradients of sodium chloride. Sepharose 200-212 fibroblast growth factor 2 Homo sapiens 171-175 8480910-1 1993 A novel electrophoresis system using agarose gel has been developed for the separation and as an aid in the classification of antibiotics. Sepharose 37-44 activation induced cytidine deaminase Homo sapiens 97-100 8460942-3 1993 Sepharose-6B gel filtration experiments with combinations of nonlabeled and 14C reductively methylated initiation factors (eIF-4A, eIF-4B, eIF-4F, and eIF-3) provide evidence that both eIF-4B and eIF-4F, but not eIF-4A, interact with ribosomes in the presence of specific factors and ATP. Sepharose 0-9 eukaryotic initiation factor 4A-I Oryctolagus cuniculus 123-129 8460942-3 1993 Sepharose-6B gel filtration experiments with combinations of nonlabeled and 14C reductively methylated initiation factors (eIF-4A, eIF-4B, eIF-4F, and eIF-3) provide evidence that both eIF-4B and eIF-4F, but not eIF-4A, interact with ribosomes in the presence of specific factors and ATP. Sepharose 0-9 eukaryotic translation initiation factor 4B Oryctolagus cuniculus 131-137 8460942-3 1993 Sepharose-6B gel filtration experiments with combinations of nonlabeled and 14C reductively methylated initiation factors (eIF-4A, eIF-4B, eIF-4F, and eIF-3) provide evidence that both eIF-4B and eIF-4F, but not eIF-4A, interact with ribosomes in the presence of specific factors and ATP. Sepharose 0-9 eukaryotic translation initiation factor 4B Oryctolagus cuniculus 185-191 8460942-3 1993 Sepharose-6B gel filtration experiments with combinations of nonlabeled and 14C reductively methylated initiation factors (eIF-4A, eIF-4B, eIF-4F, and eIF-3) provide evidence that both eIF-4B and eIF-4F, but not eIF-4A, interact with ribosomes in the presence of specific factors and ATP. Sepharose 0-9 eukaryotic initiation factor 4A-I Oryctolagus cuniculus 212-218 8443391-7 1993 Analysis by crossed immunoelectrophoresis in the presence of heparin and affinity chromatography on heparin-Sepharose demonstrated that the propositus" AT III was composed of two populations, one having no affinity for heparin and the other binding heparin normally. Sepharose 108-117 serpin family C member 1 Homo sapiens 152-158 8485376-0 1993 High performance gel filtration using monodisperse highly cross-linked agarose as a one-step system for mucin purification. Sepharose 71-78 LOC100508689 Homo sapiens 104-109 8489739-4 1993 For this purpose G- and F-actin or actin in complex with deoxyribonuclease I (DNase I) was passed over ADP-agarose which was found to retain all three forms of actin. Sepharose 107-114 deoxyribonuclease 1 Homo sapiens 57-76 8449919-9 1993 Both the sucrose gradient experiments and m7GTP-Sepharose 4B assays demonstrated that the [14C]p46 subunit of [14C]eIF-4F was displaced in the presence of eIF-4AI or eIF-4AII and that mouse [14C]eIF-4AI or [14C]eIF-4AII became incorporated into the eIF-4F complex in the same manner as rabbit reticulocyte eIF-4A. Sepharose 48-57 eukaryotic translation initiation factor 4A2 Mus musculus 166-174 8449919-9 1993 Both the sucrose gradient experiments and m7GTP-Sepharose 4B assays demonstrated that the [14C]p46 subunit of [14C]eIF-4F was displaced in the presence of eIF-4AI or eIF-4AII and that mouse [14C]eIF-4AI or [14C]eIF-4AII became incorporated into the eIF-4F complex in the same manner as rabbit reticulocyte eIF-4A. Sepharose 48-57 eukaryotic translation initiation factor 4A1 Mus musculus 166-173 8449919-9 1993 Both the sucrose gradient experiments and m7GTP-Sepharose 4B assays demonstrated that the [14C]p46 subunit of [14C]eIF-4F was displaced in the presence of eIF-4AI or eIF-4AII and that mouse [14C]eIF-4AI or [14C]eIF-4AII became incorporated into the eIF-4F complex in the same manner as rabbit reticulocyte eIF-4A. Sepharose 48-57 eukaryotic initiation factor 4A-I Oryctolagus cuniculus 155-161 8320135-3 1993 The amplified TCR cDNA was purified by agarose gel electrophoresis and subjected to direct sequencing. Sepharose 39-46 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 14-17 8486604-8 1993 The binding of NBP60 to nucleoplasmin-Sepharose was inhibited by 50% in the presence of 0.12 mM T-peptide. Sepharose 38-47 lamin B receptor Rattus norvegicus 15-20 8486610-2 1993 A major component of the family, p62, was purified from a salt extract of rat liver nuclear envelopes by wheat germ agglutinin-Sepharose affinity chromatography and DEAE-anion exchange HPLC. Sepharose 127-136 KH RNA binding domain containing, signal transduction associated 1 Rattus norvegicus 33-36 8486612-1 1993 In order to characterize the intracellular processing event of lysosomal cathepsin B, the proenzyme was purified from the rat liver microsomal contents using a Con A-Sepharose column, a Sepharose-Gly-Phe-GlySc column, and an anti-cathepsin B IgG column. Sepharose 166-175 cathepsin B Rattus norvegicus 73-84 8486612-1 1993 In order to characterize the intracellular processing event of lysosomal cathepsin B, the proenzyme was purified from the rat liver microsomal contents using a Con A-Sepharose column, a Sepharose-Gly-Phe-GlySc column, and an anti-cathepsin B IgG column. Sepharose 186-195 cathepsin B Rattus norvegicus 73-84 8486612-4 1993 When the purified proenzyme was incubated with the cathepsin B-free tritosomal contents, prepared by treatment of the tritosomal contents with anti-cathepsin B IgG Sepharose, at pH 3.0, 30 degrees C, a remarkable increase of enzymatic activity was observed. Sepharose 164-173 cathepsin B Rattus norvegicus 51-62 8486612-7 1993 When the proenzyme was incubated with the cathepsins B- and D-free tritosomal contents, prepared by treatment of the cathepsin B-free tritosomal contents with anti-cathepsin D IgG Sepharose, the processing and activation did not occur. Sepharose 180-189 cathepsin D Rattus norvegicus 164-175 8382315-4 1993 The expressed BMRF1 gene products were purified to near homogeneity from the nuclear extract of the recombinant baculovirus-infected insect cells by double-stranded DNA-cellulose column chromatography followed by heparin-agarose column chromatography. Sepharose 221-228 BMRF1 Human gammaherpesvirus 4 14-19 8483891-3 1993 Three pairs of primers for the amplification of beta-actin cDNAs of 83,260, and 540 base pairs were used to evaluate the length of mRNA that could be analyzed; the results indicate the consistent amplification of cDNA for the short- and intermediate-sized fragments as revealed by ethidium bromide fluorescence of agarose gel-resolved PCR products. Sepharose 314-321 POTE ankyrin domain family member F Homo sapiens 48-58 7683917-10 1993 Transformed Escherichia coli expressed a functional soybean AAT isozyme, which comigrated with the soybean AAT5 isozyme during agarose gel electrophoresis. Sepharose 127-134 aspartate aminotransferase glyoxysomal isozyme AAT1 precursor Glycine max 60-63 8439314-1 1993 Rat hepatic GST 7-7 expression in cytosol and in the S-hexylglutathione-agarose affinity purified fraction of cytosol from saline- (control) and pyrrole-treated animals was examined using metabolic activity, SDS-PAGE, immunoblot, and HPLC analyses. Sepharose 72-79 glutathione S-transferase pi 1 Rattus norvegicus 12-19 7681451-4 1993 IgG was purified from free protein extracts by protein G Sepharose affinity chromatography and anti-MBP was further isolated from purified IgG by antigen specific (MBP) Sepharose affinity chromatography. Sepharose 169-178 myelin basic protein Homo sapiens 100-103 8450740-3 1993 T lymphocytes in standard culture and inside agarose beads exhibit comparable viability, and similar extent and kinetics of DNA synthesis and interleukin-2 secretion. Sepharose 45-52 interleukin 2 Homo sapiens 142-155 8492909-11 1993 GluR1 was immunoaffinity-purified using subunit-specific antibodies against both N-terminal (253-267) and C-terminal (877-889) residues, covalently attached to protein A-agarose. Sepharose 170-177 glutamate ionotropic receptor AMPA type subunit 1 Rattus norvegicus 0-5 8446623-2 1993 Beginning with taurocholate-solubilized microsomal membranes, LTC4 synthase was chromatographically resolved by (i) anion exchange, (ii) affinity chromatography (through a resin of biotinylated LTC2 immobilized on streptavidin-agarose), and then (iii) gel filtration. Sepharose 227-234 leukotriene C4 synthase Homo sapiens 62-75 8382699-5 1993 We report here the purification of CISP to apparent homogeneity with an overall yield of 43% using a combination of heparin-agarose and Mono-Q chromatographies. Sepharose 124-131 thrombospondin 2 Bos taurus 35-39 8439564-9 1993 This translation product was immunoprecipitated with rabbit anti-serum to human TC II and was able to bind to Cbl-Sepharose beads. Sepharose 114-123 transcobalamin 2 Homo sapiens 80-85 8439564-9 1993 This translation product was immunoprecipitated with rabbit anti-serum to human TC II and was able to bind to Cbl-Sepharose beads. Sepharose 114-123 Cbl proto-oncogene Homo sapiens 110-113 8429005-7 1993 On a heparin-Sepharose column, LPL 5G eluted at 0.96 M NaCl compared with 1.35 M for wild-type LPL. Sepharose 13-22 LOW QUALITY PROTEIN: lipoprotein lipase Cricetulus griseus 31-34 8439332-0 1993 Identification of the fatty acid binding site on glutathione S-transferase P by immobilization to fatty acid-linked sepharose. Sepharose 116-125 glutathione S-transferase pi 1 Homo sapiens 49-76 8486604-11 1993 NBP60 extracted from the nuclear envelope was purified by nucleoplasmin-Sepharose affinity chromatography following hydroxyapatite high performance liquid chromatography. Sepharose 72-81 lamin B receptor Rattus norvegicus 0-5 8439332-4 1993 GST-P tightly bound the fatty acid-linked Sepharoses (CH3(CH2)nCOOH, n = 4 approximately 16), and the site was determined to be residues 121-156 from the amino terminus by tryptic digestion of GST-P bound to a fatty acid-linked Sepharose. Sepharose 42-52 glutathione S-transferase pi 1 Homo sapiens 0-5 8439332-4 1993 GST-P tightly bound the fatty acid-linked Sepharoses (CH3(CH2)nCOOH, n = 4 approximately 16), and the site was determined to be residues 121-156 from the amino terminus by tryptic digestion of GST-P bound to a fatty acid-linked Sepharose. Sepharose 42-52 glutathione S-transferase pi 1 Homo sapiens 193-198 8439332-4 1993 GST-P tightly bound the fatty acid-linked Sepharoses (CH3(CH2)nCOOH, n = 4 approximately 16), and the site was determined to be residues 121-156 from the amino terminus by tryptic digestion of GST-P bound to a fatty acid-linked Sepharose. Sepharose 42-51 glutathione S-transferase pi 1 Homo sapiens 0-5 8439332-4 1993 GST-P tightly bound the fatty acid-linked Sepharoses (CH3(CH2)nCOOH, n = 4 approximately 16), and the site was determined to be residues 121-156 from the amino terminus by tryptic digestion of GST-P bound to a fatty acid-linked Sepharose. Sepharose 42-51 glutathione S-transferase pi 1 Homo sapiens 193-198 8486606-1 1993 CK-II has been partially purified from a 1.5 M KCl extract of unfertilized sea urchin eggs by means of DEAE-cellulose column chromatography, gel filtration on Sephacryl S300, and heparin-agarose column chromatography, successively. Sepharose 187-194 casein kinase 2 alpha 1 Homo sapiens 0-5 8431480-15 1993 ECE activity in M1 was able to bind to a concanavalin A-agarose column and was eluted by alpha-methyl-D-glucoside, indicating that the ECE is glycosylated. Sepharose 56-63 endothelin converting enzyme 1 Homo sapiens 0-3 8444150-5 1993 The recombinant gal-T was detergent solubilized and subsequently purified by affinity chromatography on N-acetylglucosamine-derivatized Sepharose followed by alpha-lactalbumin-Sepharose. Sepharose 136-145 galactose-1-phosphate uridylyltransferase Homo sapiens 16-21 8444150-5 1993 The recombinant gal-T was detergent solubilized and subsequently purified by affinity chromatography on N-acetylglucosamine-derivatized Sepharose followed by alpha-lactalbumin-Sepharose. Sepharose 176-185 galactose-1-phosphate uridylyltransferase Homo sapiens 16-21 8428923-8 1993 GPRP specifically eluted t-PA, which was previously bound to a fibrin-Sepharose column, and also inhibited t-PA binding to fibrin-Sepharose in a concentration-dependent manner. Sepharose 70-79 plasminogen activator, tissue type Homo sapiens 25-29 8428923-8 1993 GPRP specifically eluted t-PA, which was previously bound to a fibrin-Sepharose column, and also inhibited t-PA binding to fibrin-Sepharose in a concentration-dependent manner. Sepharose 130-139 plasminogen activator, tissue type Homo sapiens 107-111 8428931-1 1993 Recombinant human 5-lipoxygenase was expressed in Escherichia coli and purified to more than 95% homogeneity by ammonium sulfate precipitation and agarose-ATP column chromatography. Sepharose 147-154 arachidonate 5-lipoxygenase Homo sapiens 18-32 8457652-10 1993 These clones secreted biologically active recombinant human fibrinogen, which was purified from serum-free culture media by protamine-Sepharose chromatography. Sepharose 134-143 fibrinogen beta chain Homo sapiens 60-70 8429008-3 1993 Quick I, Quick II, and alpha-thrombin were eluted at the same salt concentration from heparin-Sepharose suggesting that the putative heparin-binding site (also termed anion binding exosite-II) is functional. Sepharose 94-103 coagulation factor II, thrombin Homo sapiens 29-37 8384496-1 1993 The region and mechanism of urinary protein C inhibitor (PCI) binding to fibrin(ogen) were examined using fibrin(ogen)-Sepharose and ligand blotting. Sepharose 119-128 serpin family A member 5 Homo sapiens 36-55 8384496-2 1993 Urinary PCI bound to fibrin(ogen)-Sepharose in a heparin-dependent manner at a level about 1.6-fold higher to fibrin-Sepharose than to fibrinogen-Sepharose. Sepharose 34-43 fibrinogen beta chain Homo sapiens 135-145 8384496-3 1993 Scatchard analysis of the binding between urinary PCI and fibrin(ogen)-Sepharose showed that the Kd for fibrin-Sepharose and fibrinogen-Sepharose were 4.0 nM and 5.7 nM respectively. Sepharose 71-80 fibrinogen beta chain Homo sapiens 125-135 8473417-5 1993 Both HLA-DR expression and inhibition of proliferation were found to be directly dependent on the dose of IFN-gamma that was allowed to diffuse in the agarose beneath the spheroids. Sepharose 151-158 interferon gamma Homo sapiens 106-115 8443798-5 1993 The expression of the p120 protein was inhibited by 44% in the antisense-transfected MCF-7pSVX021 cells; a 56% inhibition of cell-growth and a reduced colony formation in soft agarose were also observed. Sepharose 176-183 catenin delta 1 Homo sapiens 22-26 8382586-4 1993 The material had the characteristics of calmodulin in that it stimulated both phosphodiesterase and erythrocyte Ca(2+)-ATPase activities, cross-reacted with and was neutralized by anti-calmodulin antibody, was adsorbed by phenothiazine-Sepharose and was heat-stable. Sepharose 236-245 calmodulin 1 Homo sapiens 40-50 8382586-7 1993 Calmodulin-agarose removed calmodulin-enhancing activity from the supernatant. Sepharose 11-18 calmodulin 1 Homo sapiens 0-10 8382586-7 1993 Calmodulin-agarose removed calmodulin-enhancing activity from the supernatant. Sepharose 11-18 calmodulin 1 Homo sapiens 27-37 7679105-6 1993 Furthermore, the forced secretion of biologically active FGF-1 resulted in NIH 3T3 cell transformation as demonstrated by altered morphology in vitro, the formation of discrete colonies in soft agarose, growth under serum-free conditions, and ability to rapidly form highly vascular tumors in vivo. Sepharose 194-201 fibroblast growth factor 1 Mus musculus 57-62 8473433-3 1993 As W7 is able to penetrate the zona pellucida and enter the cells, calmodulin antibody can traverse the zona but not enter the cells and W7-agarose cannot traverse the zona, we have deduced that the calmodulin which appears relevant to embryo division may be both intracellular and intrazonal but not extrazonal. Sepharose 140-147 calmodulin 1 Homo sapiens 199-209 8459200-2 1993 Immunoreactive-bFGF (ir-bFGF) bound to heparin-Sepharose affinity columns eluted with 1.8-2.0 mol NaCl/l and had a molecular weight of approximately 17,000. Sepharose 47-56 fibroblast growth factor 2 Homo sapiens 15-19 8459200-2 1993 Immunoreactive-bFGF (ir-bFGF) bound to heparin-Sepharose affinity columns eluted with 1.8-2.0 mol NaCl/l and had a molecular weight of approximately 17,000. Sepharose 47-56 fibroblast growth factor 2 Homo sapiens 24-28 7678252-7 1993 Furthermore, TAT in patient plasmas (disseminated intravascular coagulation and sepsis) was found to bind to heparin-Sepharose, indicating that this endogenously formed TAT was also associated with VN. Sepharose 117-126 tyrosine aminotransferase Homo sapiens 13-16 8380340-6 1993 Ubiquitin-activating enzyme (E1), the first enzyme in the cascade of ubiquitin ligation, was purified over 3000-fold from a rabbit reticulocyte lysate using Ubiquitin-Sepharose, and showed ATP-PPi exchange activity. Sepharose 167-176 ubiquitin-like modifier-activating enzyme 1 Oryctolagus cuniculus 0-32 8380340-6 1993 Ubiquitin-activating enzyme (E1), the first enzyme in the cascade of ubiquitin ligation, was purified over 3000-fold from a rabbit reticulocyte lysate using Ubiquitin-Sepharose, and showed ATP-PPi exchange activity. Sepharose 167-176 ubiquitin Oryctolagus cuniculus 69-78 8380340-6 1993 Ubiquitin-activating enzyme (E1), the first enzyme in the cascade of ubiquitin ligation, was purified over 3000-fold from a rabbit reticulocyte lysate using Ubiquitin-Sepharose, and showed ATP-PPi exchange activity. Sepharose 167-176 ubiquitin Oryctolagus cuniculus 0-9 21573527-3 1993 MIS-transfection inhibited proliferation of CHO cells in double-layer agarose, tumor spheroid, and murine subrenal capsule assays, as well as growth of CHO and OM431 cells in pulmonary metastasis studies. Sepharose 70-77 anti-Mullerian hormone Homo sapiens 0-3 7678252-7 1993 Furthermore, TAT in patient plasmas (disseminated intravascular coagulation and sepsis) was found to bind to heparin-Sepharose, indicating that this endogenously formed TAT was also associated with VN. Sepharose 117-126 tyrosine aminotransferase Homo sapiens 169-172 7678252-7 1993 Furthermore, TAT in patient plasmas (disseminated intravascular coagulation and sepsis) was found to bind to heparin-Sepharose, indicating that this endogenously formed TAT was also associated with VN. Sepharose 117-126 vitronectin Homo sapiens 198-200 8424779-1 1993 Lysosomal beta-mannosidase was purified 160,000-fold in 24% yield from bovine kidney by a four-step purification procedure, which included concanavalin A-Sepharose, immunoaffinity, TSK-butyl and h.p.l.c. Sepharose 154-163 mannosidase beta Bos taurus 10-26 8422470-5 1993 Protein G sepharose absorption showed that the anti-HLA V region-reactive antibodies were IgG. Sepharose 10-19 major histocompatibility complex, class I, V (pseudogene) Homo sapiens 52-57 8285022-8 1993 When the DNA of leukemic cells treated with TNF was separated on agarose, no fragments characteristic of apoptosis were visible. Sepharose 65-72 tumor necrosis factor Homo sapiens 44-47 8424457-5 1993 When chromatographed on 4% agarose (A-15M) in the presence of ethylenediamine tetraacetic acid, the solubilized human proteinosis SP-D eluted near the void volume and earlier than rat SP-D dodecamers or human SP-D multimers in the lavage supernatant. Sepharose 27-34 surfactant protein D Homo sapiens 130-134 8422024-2 1993 The CK-MB-like activity present in serum samples from this patient was definitively identified as an immunoglobulin A complex of CK-MM (macro CK, type 1) using CK isoenzyme agarose gel electrophoresis after pretreating the patient"s serum sample with either anti-immunoglobulin A antiserum or anti-CK-M antibody reagent. Sepharose 173-180 creatine kinase, M-type Homo sapiens 4-8 8416739-4 1993 VPF was isolated from serum-free tumor cell conditioned medium using heparin-Sepharose affinity chromatography. Sepharose 77-86 vascular endothelial growth factor A Mus musculus 0-3 8434786-3 1993 GSH-Px was not retained by either of these columns but selenoprotein P was retained by heparin-Sepharose and albumin by reactive blue. Sepharose 95-104 selenoprotein P Rattus norvegicus 55-70 8434786-4 1993 After the two columns were separated, selenoprotein P was eluted with heparin from heparin-Sepharose and albumin eluted from reactive blue with high salt. Sepharose 91-100 selenoprotein P Rattus norvegicus 38-53 8010870-1 1993 Two monoclonal antibodies, coupled to Sepharose 4B, were used for rapid isolation of the human haptoglobin in a single chromatographic step. Sepharose 38-47 haptoglobin Homo sapiens 95-106 8423369-5 1993 Subsequent NaCl gradient FPLC on Hiload S-Sepharose, equilibrated in 10 mM potassium phosphate, 5 mM EDTA, pH 7.0, provides both factor B and beta 2I in homogeneous form. Sepharose 42-51 proteasome 20S subunit beta 10 Homo sapiens 142-149 8403897-2 1993 Affinity chromatography of "Enamelin Extracts" of developing bovine molar enamel on CNBr activated Sepharose 4B to which polyclonal antibodies of whole bovine serum and fetuin were cross-linked, revealed that at most, only 1-2% of the proteins in the extracts were not bound to the columns. Sepharose 99-108 enamelin Bos taurus 28-36 8382130-10 1993 Human renin in the hRN8-12 mouse kidney was bound to a concanavalin A-Sepharose column, and was eluted with alpha-methyl-D-mannoside, showing that this renin is glycosylated, as is native human renin. Sepharose 70-79 renin Homo sapiens 6-11 8382130-10 1993 Human renin in the hRN8-12 mouse kidney was bound to a concanavalin A-Sepharose column, and was eluted with alpha-methyl-D-mannoside, showing that this renin is glycosylated, as is native human renin. Sepharose 70-79 renin Homo sapiens 152-157 8382130-10 1993 Human renin in the hRN8-12 mouse kidney was bound to a concanavalin A-Sepharose column, and was eluted with alpha-methyl-D-mannoside, showing that this renin is glycosylated, as is native human renin. Sepharose 70-79 renin Homo sapiens 152-157 8402795-4 1993 Phosphoprotein, proteoglycan and gamma-carboxyglutamate-containing protein (Gla-protein) of the osteocalcin type were covalently coupled to AH-Sepharose beads, and these were implanted subcutaneously in minipigs for periods between 3 and 24 weeks. Sepharose 143-152 galactosidase, alpha Rattus norvegicus 76-79 8380742-4 1993 F-TCF was purified from conditioned medium by a combination of UF-concentration, CM sephadex C-50, Con A sepharose, Mono S cation-exchange and heparin sepharose chromatography and exhibited a molecular mass (M(r)) of 76 to 80 kD on SDS-PAGE under non-reducing conditions. Sepharose 105-114 hepatocyte growth factor Homo sapiens 0-5 8391444-7 1993 The uptake of glucose-6-phosphate has taken place in liposomes of Sepharose affinity purified products suggesting that this preparation may be a complex of PH and glucose-6-phosphate translocase. Sepharose 66-75 solute carrier family 37 member 4 Homo sapiens 163-194 8087205-1 1993 Two major 2-aminoadipate aminotransferase (AadAT) activities of human liver extract were separated by DEAE-Sepharose column chromatography. Sepharose 107-116 aminoadipate aminotransferase Homo sapiens 10-41 8087205-1 1993 Two major 2-aminoadipate aminotransferase (AadAT) activities of human liver extract were separated by DEAE-Sepharose column chromatography. Sepharose 107-116 aminoadipate aminotransferase Homo sapiens 43-48 8380742-4 1993 F-TCF was purified from conditioned medium by a combination of UF-concentration, CM sephadex C-50, Con A sepharose, Mono S cation-exchange and heparin sepharose chromatography and exhibited a molecular mass (M(r)) of 76 to 80 kD on SDS-PAGE under non-reducing conditions. Sepharose 151-160 hepatocyte growth factor Homo sapiens 0-5 8294188-5 1993 Between 15 and 22 bands were observed in agarose gel electrophoresis with AHV DNA cut by BamHI, EcoRI, PstI or BglII. Sepharose 41-48 serine peptidase inhibitor Kazal type 1 Homo sapiens 103-107 8444404-4 1993 The binding region was determined to be at around 142-157 residues from amino terminus by the studies of GST-P binding to fatty acid-linked Sepharose and affinity labelings with either fluorescent fatty acid or bilirubin. Sepharose 140-149 glutathione S-transferase pi 1 Homo sapiens 105-110 8318251-3 1993 Partial purification of the immunoreactive 18 kDa BMP-2 from Xenopus embryo extract by gel filtration, heparin-Sepharose affinity chromatography and preparative SDS-PAGE resulted in co-purification and identification of higher molecular forms of BMP-2 of 110 kDa and 36 kDa. Sepharose 111-120 bone morphogenetic protein 2 S homeolog Xenopus laevis 50-55 8423225-2 1993 Rhesus lipoprotein(a) (Lp[a]) binds less efficiently than human Lp(a) to lysine-Sepharose and to cultured U937 cells. Sepharose 80-89 lipoprotein(a) Homo sapiens 7-21 8423225-2 1993 Rhesus lipoprotein(a) (Lp[a]) binds less efficiently than human Lp(a) to lysine-Sepharose and to cultured U937 cells. Sepharose 80-89 lipoprotein(a) Homo sapiens 64-68 8421063-5 1993 Purified CSVTCG binding protein (CBP) bound either CSVTCG- or TSP-Sepharose but showed little interaction with either VCTGSC- or BSA-Sepharose. Sepharose 66-75 CREB binding protein Homo sapiens 9-31 8452629-4 1993 These data demonstrate that: (1) IgG 3 antibodies can be partly responsible for lymphocytotoxic anti-HLA reactivity in some sensitized renal transplant recipients and (2) at least in this patient category, in vitro immunoadsorption with protein G is more efficient than protein A sepharose columns in completely removing anti-HLA IgG antibodies from sera. Sepharose 280-289 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 33-38 8421063-5 1993 Purified CSVTCG binding protein (CBP) bound either CSVTCG- or TSP-Sepharose but showed little interaction with either VCTGSC- or BSA-Sepharose. Sepharose 66-75 CREB binding protein Homo sapiens 33-36 8421063-5 1993 Purified CSVTCG binding protein (CBP) bound either CSVTCG- or TSP-Sepharose but showed little interaction with either VCTGSC- or BSA-Sepharose. Sepharose 66-75 thrombospondin 1 Homo sapiens 62-65 8443592-2 1993 Baculoviral-insect cell-derived recombinant FGF-R1 was phosphorylated and fragmented with trypsin while immobilized on heparin-agarose beads. Sepharose 127-134 fibroblast growth factor receptor 1 Homo sapiens 44-50 7694351-1 1993 Serum alpha-fetoprotein (AFP) from cord blood and from patients with hepatitis, cirrhosis, hepatocellular carcinoma, gastrointestinal tumors and yolk sac tumor was analyzed by extended agarose gel electrophoresis coupled with our sensitive detection method of antibody-affinity blotting. Sepharose 185-192 alpha fetoprotein Homo sapiens 6-23 1334488-2 1992 A novel human tissue kallikrein inhibitor designated as kallistatin has been purified from plasma to apparent homogeneity by polyethylene glycol fractionation and successive chromatography on heparin-Agarose, DEAE-Sepharose, hydroxylapatite, and phenyl-Superose columns. Sepharose 200-207 serpin family A member 4 Homo sapiens 56-67 1335236-1 1992 Angiotensin converting enzyme (ACE; EC 3.4.15.1) was purified from porcine kidney and lung (endothelial isoenzyme) and testis (testicular isoenzyme) by affinity chromatography on lisinopril-2.8 nm-Sepharose. Sepharose 197-206 angiotensin-converting enzyme Sus scrofa 0-29 1335236-1 1992 Angiotensin converting enzyme (ACE; EC 3.4.15.1) was purified from porcine kidney and lung (endothelial isoenzyme) and testis (testicular isoenzyme) by affinity chromatography on lisinopril-2.8 nm-Sepharose. Sepharose 197-206 angiotensin-converting enzyme Sus scrofa 31-34 1475180-4 1992 The effects of covalently bound BPDE residues on the electrophoretic mobilities of the RF I DNA form in agarose gels were investigated in detail in the range of binding ratios rb approximately 0.0-0.06 (covalently bound BPDE residues/nucleotide). Sepharose 104-111 ring finger protein 34 Homo sapiens 87-91 1280270-3 1992 Yolk vitronectin was purified 2,500-fold from chick egg yolk by a combination of hydroxylapatite, DEAE-cellulose, and anti-vitronectin-Sepharose column chromatographies. Sepharose 135-144 vitronectin Gallus gallus 5-16 1280270-3 1992 Yolk vitronectin was purified 2,500-fold from chick egg yolk by a combination of hydroxylapatite, DEAE-cellulose, and anti-vitronectin-Sepharose column chromatographies. Sepharose 135-144 vitronectin Gallus gallus 123-134 1292684-1 1992 The relationship between the concentration of serum amyloid protein (SAP) isolated from human serum and the parameters of the protein elution during gel-filtration and alos with the efficiency of Ca(2+)-dependent SAP binding with sepharose 4B was studied. Sepharose 230-239 amyloid P component, serum Homo sapiens 46-67 1292684-1 1992 The relationship between the concentration of serum amyloid protein (SAP) isolated from human serum and the parameters of the protein elution during gel-filtration and alos with the efficiency of Ca(2+)-dependent SAP binding with sepharose 4B was studied. Sepharose 230-239 amyloid P component, serum Homo sapiens 69-72 1292684-1 1992 The relationship between the concentration of serum amyloid protein (SAP) isolated from human serum and the parameters of the protein elution during gel-filtration and alos with the efficiency of Ca(2+)-dependent SAP binding with sepharose 4B was studied. Sepharose 230-239 amyloid P component, serum Homo sapiens 213-216 1298437-1 1992 Human transferrin receptor was isolated from Triton X-100 solubilized placental plasma membranes by a rapid one-step chromatographic procedure based on immunoadsorption of the receptor-transferrin complex on anti-transferrin Sepharose and lectin-affinity on wheat germ agglutinin. Sepharose 225-234 transferrin receptor Homo sapiens 6-26 1299558-8 1992 The fusion protein was purified by affinity chromatography on an anti-beta-galactosidase-Sepharose column. Sepharose 89-98 galactosidase beta 1 Homo sapiens 70-88 1458597-2 1992 We have used the activity patterns of liver and bone isoenzymes of alkaline phosphatase (ALP), separated by agarose gel electrophoresis, to detect early metastasis. Sepharose 108-115 alkaline phosphatase, placental Homo sapiens 67-87 1458597-2 1992 We have used the activity patterns of liver and bone isoenzymes of alkaline phosphatase (ALP), separated by agarose gel electrophoresis, to detect early metastasis. Sepharose 108-115 alkaline phosphatase, placental Homo sapiens 89-92 1284814-2 1992 We show the occurrence of saturable, temperature, pH, and calcium dependent carbohydrate-specific interactions between recombinant precursor gp160 (rgp160) and two affinity matrices: D-mannose-divinylsulfone-agarose, and natural glycoprotein, fetuin, also coupled to agarose. Sepharose 208-215 glutamyl aminopeptidase Homo sapiens 141-146 1284814-2 1992 We show the occurrence of saturable, temperature, pH, and calcium dependent carbohydrate-specific interactions between recombinant precursor gp160 (rgp160) and two affinity matrices: D-mannose-divinylsulfone-agarose, and natural glycoprotein, fetuin, also coupled to agarose. Sepharose 267-274 glutamyl aminopeptidase Homo sapiens 141-146 1334944-6 1992 After reverse transcription of mRNA from this case to cDNA and amplification by the polymerase chain reaction, we identified an amplification product that co-migrated with IL-5-positive controls in an agarose gel. Sepharose 201-208 interleukin 5 Homo sapiens 172-176 1391338-7 1992 Finally, Sertoli cells were incubated with 35S-cysteine and the conditioned medium was put over a TTR-Sepharose column to isolate the radioactive protein, as characterized above. Sepharose 102-111 transthyretin Rattus norvegicus 98-101 1294810-1 1992 The delta-subspecies of protein kinase C (PKC) was purified to near homogeneity from the Triton X-100 extract of the rat brain particulate fraction by successive chromatographies on S-Sepharose Fast Flow, Phenyl 5PW, Heparin 5PW, hydroxyapatite, and Mono Q columns. Sepharose 184-193 protein kinase C, gamma Rattus norvegicus 24-40 1294810-1 1992 The delta-subspecies of protein kinase C (PKC) was purified to near homogeneity from the Triton X-100 extract of the rat brain particulate fraction by successive chromatographies on S-Sepharose Fast Flow, Phenyl 5PW, Heparin 5PW, hydroxyapatite, and Mono Q columns. Sepharose 184-193 protein kinase C, gamma Rattus norvegicus 42-45 8069555-5 1993 Determination of the interaction between avidin-pLys460-]bio-transferrin] and DNA was carried out by nitro cellulose filter binding and agarose gel retardation assays. Sepharose 136-143 transferrin Homo sapiens 61-72 1485730-5 1992 Moreover, one of the main findings here reported is the failure of anti-human apo A-1 murine monoclonal antibody and monoclonal antibody mixture to precipitate free apo A-1 in agarose systems. Sepharose 176-183 apolipoprotein A1 Homo sapiens 165-172 1335242-3 1992 Carboxymethylated HBNF and MK, which retain affinity for heparin-Sepharose, do not exhibit anti-viral activities. Sepharose 65-74 pleiotrophin Homo sapiens 18-22 1487516-3 1992 Over-expressed Ck-beta-galactosidase fusion protein was purified using monoclonal antibodies immobilised on Sepharose 4B. Sepharose 108-117 galactosidase beta 1 Homo sapiens 18-36 1487520-2 1992 The dye leaching from Remazol Yellow GGL-Sepharose used for the affinity chromatography of human plasma transthyretin was quantitatively studied by a sensitive competitive enzyme immunoassay. Sepharose 41-50 transthyretin Homo sapiens 104-117 1335701-5 1992 In HUVE cells exposed to histamine we estimated actin-gelsolin binding by quantitating actin and gelsolin, immunoprecipitated with anti-gelsolin Sepharose. Sepharose 145-154 gelsolin Homo sapiens 54-62 1363192-3 1992 Using different concentrations of methyl-alpha-D-mannopyranoside for the elution of GGT from Con A-Sepharose column, the enzyme from brain regions could be separated into five fractions, two of which contained about 75% of the total GGT activity without regional differences in elution profiles. Sepharose 99-108 gamma-glutamyltransferase 1 Rattus norvegicus 84-87 1482396-2 1992 Transferrin receptors were isolated from the solubilized membranes by affinity chromatography on diferric transferrin-coupled Sepharose 4B. Sepharose 126-138 transferrin Homo sapiens 0-11 1482396-2 1992 Transferrin receptors were isolated from the solubilized membranes by affinity chromatography on diferric transferrin-coupled Sepharose 4B. Sepharose 126-138 transferrin Homo sapiens 106-117 1473607-2 1992 In the present study, we isolated the two forms of proacrosin from acid extracts (pH 3.0) of cauda epididymal bovine spermatozoa by ammonium sulfate fractionation, gel filtration on Sephadex G-150 and affinity chromatography on Concanavalin A Sepharose 4B. Sepharose 243-252 acrosin Homo sapiens 51-61 1289411-3 1992 Al-IFN-alpha is a mixture of both glycosylated and unglycosylated molecules as shown by separation on Concanavalin-A Sepharose. Sepharose 117-126 interferon alpha 1 Homo sapiens 3-12 1460414-3 1992 The human serum MBP fraction was obtained by sequential affinity chromatography on mannan-Sepharose, anti-IgM-Sepharose and anti-MBP-Sepharose in the presence of calcium ions. Sepharose 90-99 myelin basic protein Homo sapiens 16-19 1460414-3 1992 The human serum MBP fraction was obtained by sequential affinity chromatography on mannan-Sepharose, anti-IgM-Sepharose and anti-MBP-Sepharose in the presence of calcium ions. Sepharose 110-119 myelin basic protein Homo sapiens 16-19 1460414-3 1992 The human serum MBP fraction was obtained by sequential affinity chromatography on mannan-Sepharose, anti-IgM-Sepharose and anti-MBP-Sepharose in the presence of calcium ions. Sepharose 110-119 myelin basic protein Homo sapiens 16-19 1460414-5 1992 The C1s-like serine protease, designated MBP-associated serine protease (MASP), was separated from MBP by rechromatography on anti-MBP-Sepharose in the presence of ethylenediaminetetraacetic acid. Sepharose 135-144 MBL associated serine protease 1 Homo sapiens 41-71 1460414-5 1992 The C1s-like serine protease, designated MBP-associated serine protease (MASP), was separated from MBP by rechromatography on anti-MBP-Sepharose in the presence of ethylenediaminetetraacetic acid. Sepharose 135-144 MBL associated serine protease 1 Homo sapiens 73-77 1460414-5 1992 The C1s-like serine protease, designated MBP-associated serine protease (MASP), was separated from MBP by rechromatography on anti-MBP-Sepharose in the presence of ethylenediaminetetraacetic acid. Sepharose 135-144 myelin basic protein Homo sapiens 41-44 1297892-2 1992 The binding of the selected compound, [3H]-VUF 4576, to calmodulin was studied according to a recently described technique using CaM agarose. Sepharose 133-140 calmodulin 1 Homo sapiens 56-66 1283096-4 1992 A single heparin-Sepharose chromatography of the ultrafiltrates yielded essentially homogenous, biologically active, recombinant rat aFGF or bFGF. Sepharose 17-26 fibroblast growth factor 1 Rattus norvegicus 133-137 1283096-4 1992 A single heparin-Sepharose chromatography of the ultrafiltrates yielded essentially homogenous, biologically active, recombinant rat aFGF or bFGF. Sepharose 17-26 fibroblast growth factor 2 Rattus norvegicus 141-145 1454793-4 1992 Binding of CBF to the origin, identified by the retarded mobility of the origin DNA fragment in agarose gels, required, in addition to ATP and the OBF1-OBF2-origin complex, a functional essential core nucleotide sequence. Sepharose 96-103 DNA-binding protein ABF1 Saccharomyces cerevisiae S288C 147-151 1454855-2 1992 We also demonstrate that p53 interacts in a calcium-dependent manner with S100b, a member of the S100 protein family involved in cell cycle progression and cell differentiation, and that such an interaction inhibits in vitro p53 phosphorylation by protein kinase C. The interaction between p53 and S100b was utilized for the purification of cellular and recombinant murine p53 by affinity chromatography with S100b-Sepharose. Sepharose 415-424 transformation related protein 53, pseudogene Mus musculus 25-28 1454855-2 1992 We also demonstrate that p53 interacts in a calcium-dependent manner with S100b, a member of the S100 protein family involved in cell cycle progression and cell differentiation, and that such an interaction inhibits in vitro p53 phosphorylation by protein kinase C. The interaction between p53 and S100b was utilized for the purification of cellular and recombinant murine p53 by affinity chromatography with S100b-Sepharose. Sepharose 415-424 S100 protein, beta polypeptide, neural Mus musculus 74-79 1454855-2 1992 We also demonstrate that p53 interacts in a calcium-dependent manner with S100b, a member of the S100 protein family involved in cell cycle progression and cell differentiation, and that such an interaction inhibits in vitro p53 phosphorylation by protein kinase C. The interaction between p53 and S100b was utilized for the purification of cellular and recombinant murine p53 by affinity chromatography with S100b-Sepharose. Sepharose 415-424 S100 calcium binding protein A1 Mus musculus 74-78 1454855-2 1992 We also demonstrate that p53 interacts in a calcium-dependent manner with S100b, a member of the S100 protein family involved in cell cycle progression and cell differentiation, and that such an interaction inhibits in vitro p53 phosphorylation by protein kinase C. The interaction between p53 and S100b was utilized for the purification of cellular and recombinant murine p53 by affinity chromatography with S100b-Sepharose. Sepharose 415-424 transformation related protein 53, pseudogene Mus musculus 225-228 1454855-2 1992 We also demonstrate that p53 interacts in a calcium-dependent manner with S100b, a member of the S100 protein family involved in cell cycle progression and cell differentiation, and that such an interaction inhibits in vitro p53 phosphorylation by protein kinase C. The interaction between p53 and S100b was utilized for the purification of cellular and recombinant murine p53 by affinity chromatography with S100b-Sepharose. Sepharose 415-424 transformation related protein 53, pseudogene Mus musculus 225-228 1454855-2 1992 We also demonstrate that p53 interacts in a calcium-dependent manner with S100b, a member of the S100 protein family involved in cell cycle progression and cell differentiation, and that such an interaction inhibits in vitro p53 phosphorylation by protein kinase C. The interaction between p53 and S100b was utilized for the purification of cellular and recombinant murine p53 by affinity chromatography with S100b-Sepharose. Sepharose 415-424 S100 protein, beta polypeptide, neural Mus musculus 298-303 1454855-2 1992 We also demonstrate that p53 interacts in a calcium-dependent manner with S100b, a member of the S100 protein family involved in cell cycle progression and cell differentiation, and that such an interaction inhibits in vitro p53 phosphorylation by protein kinase C. The interaction between p53 and S100b was utilized for the purification of cellular and recombinant murine p53 by affinity chromatography with S100b-Sepharose. Sepharose 415-424 transformation related protein 53, pseudogene Mus musculus 225-228 1454855-2 1992 We also demonstrate that p53 interacts in a calcium-dependent manner with S100b, a member of the S100 protein family involved in cell cycle progression and cell differentiation, and that such an interaction inhibits in vitro p53 phosphorylation by protein kinase C. The interaction between p53 and S100b was utilized for the purification of cellular and recombinant murine p53 by affinity chromatography with S100b-Sepharose. Sepharose 415-424 S100 protein, beta polypeptide, neural Mus musculus 298-303 1280230-3 1992 CSK and TPK-IIB once resolved from each other by heparin-Sepharose affinity chromatography, display opposite specificities toward synthetic peptides reproducing the sequences around the main phosphoacceptor residues of pp60c-src, namely Tyr-416 and Tyr-527. Sepharose 57-66 C-terminal Src kinase Homo sapiens 0-3 1333892-3 1992 Treatment of the nuclear Ah receptor complexes from both cell lines with chymotrypsin for 10 or 60 min gave lower molecular weight proteolytic products which also exhibited comparable molecular properties and salt gradient elution profiles from Sepharose columns linked to DNA. Sepharose 245-254 aryl-hydrocarbon receptor Mus musculus 25-36 1477980-9 1992 The serum EC-SOD in Group I is heterogeneous with regard to affinity for heparin-Sepharose and could be separated into three approximately equal fractions, whereas the EC-SOD in Group II is mainly one fraction with a high affinity for the column. Sepharose 81-90 superoxide dismutase 3 Homo sapiens 10-16 1445886-3 1992 Moreover, this mutant P450coh binds loosely to cytochrome b5-conjugated Sepharose 4B, whereas wild-type P450coh binds tightly. Sepharose 72-81 cytochrome b5 type A (microsomal) Mus musculus 47-60 1280240-2 1992 Rapid purification of GST::p15 in an active form by one-step glutathione-agarose chromatography was accomplished in the presence of an antioxidant. Sepharose 73-80 cyclin dependent kinase inhibitor 2B Homo sapiens 27-30 1449522-5 1992 GST purified from rat skin cytosol by GSH-agarose affinity chromatography exhibited a several-fold increase in the specific activity of enzyme with 1-chloro-2,4-dinitrobenzene (55-fold), ethacrynic acid (67-fold) and LTA4-methyl ester (12-fold) as substrates. Sepharose 42-49 glutathione S-transferase kappa 1 Homo sapiens 0-3 1489089-3 1992 NAD+- and (ADP-ribose)-derivatized agarose beads were recognized as polymer acceptors by the nuclear enzyme poly(ADP-ribose) polymerase. Sepharose 35-42 poly (ADP-ribose) polymerase 1 Rattus norvegicus 108-135 1480164-9 1992 As compared to human AdoMetDC, the trypanosomal enzyme showed weaker binding to a column of MGBG-Sepharose and also was significantly less sensitive to inhibition by MGBG and its congener ethylglyoxal bis(guanylhydrazone) (EGBG). Sepharose 97-106 adenosylmethionine decarboxylase 1 Homo sapiens 21-29 1491061-1 1992 Homogeneous human placental 17 beta-hydroxysteroid dehydrogenase was obtained by a procedure consisting of two fast protein liquid chromatographic (FPLC) steps using Phenyl-Sepharose hydrophobic interaction and Blue-Sepharose affinity columns. Sepharose 173-182 hydroxysteroid 17-beta dehydrogenase 1 Homo sapiens 18-64 1463777-4 1992 No significant loss of immunoreactivity was observed in solid-phase immunoassay, 90-95% of 111In-labeled conjugates being bound to CEA-coated Sepharose beads. Sepharose 142-151 carcinoembryonic antigen gene family Mus musculus 131-134 1419639-2 1992 Enzymatic activity of GST in cytosols was measured by determining 1-chloro-2,4-dinitrobenzene conjugation with glutathione, cytosolic GST subunits were determined by wide pore reversed phase HPLC, using a S-hexylglutathione-agarose affinity column, and isoelectric focussing. Sepharose 224-231 glutathione S-transferase kappa 1 Homo sapiens 22-25 1327522-7 1992 Gastrin stimulated the clonal growth of H510 cells in semisolid (agarose-containing) medium, increasing both the number and the size of the colonies. Sepharose 65-72 gastrin Homo sapiens 0-7 1468219-1 1992 The regional distribution of mouse aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase activities in mouse ocular tissues was examined using spectrophotometric and agarose-isoelectric focusing techniques. Sepharose 168-175 aldehyde dehydrogenase family 3, subfamily A1 Mus musculus 35-57 1468219-1 1992 The regional distribution of mouse aldehyde dehydrogenase (ALDH) and alcohol dehydrogenase activities in mouse ocular tissues was examined using spectrophotometric and agarose-isoelectric focusing techniques. Sepharose 168-175 aldehyde dehydrogenase family 3, subfamily A1 Mus musculus 59-63 1478276-1 1992 A biologically active fluorescent derivative of recombinant human basic fibroblast growth factor (bFGF) was prepared by immobilization on heparin-Sepharose 4B (HS) and derivatization with the fluorophore, Texas Red (TR). Sepharose 146-158 fibroblast growth factor 2 Homo sapiens 66-96 1478276-1 1992 A biologically active fluorescent derivative of recombinant human basic fibroblast growth factor (bFGF) was prepared by immobilization on heparin-Sepharose 4B (HS) and derivatization with the fluorophore, Texas Red (TR). Sepharose 146-158 fibroblast growth factor 2 Homo sapiens 98-102 1478445-7 1992 Additionally, a glycosylated variant of chicken growth hormone (G-cGH) was also revealed in the serum after concanavalin A-Sepharose chromatography. Sepharose 123-132 growth hormone Gallus gallus 48-62 1478445-7 1992 Additionally, a glycosylated variant of chicken growth hormone (G-cGH) was also revealed in the serum after concanavalin A-Sepharose chromatography. Sepharose 123-132 growth hormone Gallus gallus 66-69 1451914-3 1992 A protein kinase type II was purified from calf thymus chromatin using ammonium sulphate fractionation, ion exchange chromatography on DEAE and phosphocellulose and affinity chromatography on phosvitin- and casein-sepharose columns. Sepharose 214-223 protein kinase cAMP-activated catalytic subunit beta Bos taurus 2-16 1383386-5 1992 A 145-kD protein, similar in size to B lymphocyte CD21, was specifically precipitated from surface iodinated RHEK-1 cells using the HB-5a mAb, or using purified soluble gp350/220 coupled to agarose beads. Sepharose 190-197 complement C3d receptor 2 Homo sapiens 50-54 1464753-10 1992 Agarose gel electrophoresis of medium containing apoA-I that had been incubated with cholesterol-enriched fibroblasts revealed a particle with alpha to pre-beta mobility. Sepharose 0-7 apolipoprotein A1 Homo sapiens 49-55 1484504-5 1992 Osteogenin was isolated from demineralized baboon bone matrix and purified by chromatography on heparin-Sepharose, hydroxyapatite, and Sephacryl S-200. Sepharose 104-113 bone morphogenetic protein 3 Homo sapiens 0-10 1445299-3 1992 Annexin I and annexin II could be rapidly purified from a human placental membrane extract by concanavalin A-Sepharose, which indicated that these proteins contain two biantennary mannosyl residues. Sepharose 109-118 annexin A2 Homo sapiens 14-24 1328244-6 1992 Purification of the epitope-tagged beta 2-AR by immunoprecipitation as well as by alprenolol-Sepharose affinity chromatography revealed that the receptor is covalently modified with palmitic acid in the insect cells as is observed in mammalian cells. Sepharose 93-102 adrenoceptor beta 2 Homo sapiens 35-44 1356985-3 1992 Immunoprecipitation of apoenzyme-containing cell extract with protein-A-Sepharose-bound 6-HDNO- or GroEL-specific antibodies failed to reveal the formation of complexes between these proteins. Sepharose 72-81 GroEL Escherichia coli 99-104 1394043-5 1992 In addition, the immunosuppressive activity was bound to a gelatin-Sepharose affinity column, known to bind fibronectin. Sepharose 67-76 fibronectin 1 Homo sapiens 108-119 1400409-4 1992 The key to the purification procedures we used was buffer containing 0.5 M NaCl and 50% ethylene glycol, as a specific elutant of p34cdc2 kinase from p13suc1-Sepharose. Sepharose 158-167 cyclin dependent kinase 1 Homo sapiens 130-137 1400426-6 1992 Exploiting the interaction of gp330 and RAP, we purified gp330 by affinity chromatography with a column of RAP coupled to Sepharose. Sepharose 122-131 LDL receptor related protein 2 Homo sapiens 57-62 1400294-5 1992 Purification of soluble glycoprotein III using wheat germ agglutinin-Sepharose resulted in the recovery of similar proportions of glycoproteins H, J, and K and gel filtration of the eluted material in combination with immunoprecipitation revealed the presence of heteroaggregates containing all of the glycoproteins. Sepharose 69-78 clusterin Bos taurus 24-40 1391338-3 1992 First, transthyretin (TTR) bound to Sepharose 4B retained a labeled protein from media collected from Sertoli cells provided with 35S-methionine, under the same conditions as authentic RBP is bound. Sepharose 36-48 transthyretin Rattus norvegicus 7-20 1391338-3 1992 First, transthyretin (TTR) bound to Sepharose 4B retained a labeled protein from media collected from Sertoli cells provided with 35S-methionine, under the same conditions as authentic RBP is bound. Sepharose 36-48 transthyretin Rattus norvegicus 22-25 1398925-4 1992 The SCID-PBL-Hu mice with high concentrations of human IgG regularly had mono- or oligoclonal human IgG bands in serum, as demonstrated by agarose gel electrophoresis. Sepharose 139-146 protein kinase, DNA activated, catalytic polypeptide Mus musculus 4-8 1398925-4 1992 The SCID-PBL-Hu mice with high concentrations of human IgG regularly had mono- or oligoclonal human IgG bands in serum, as demonstrated by agarose gel electrophoresis. Sepharose 139-146 immunoglobulin heavy variable V1-62 Mus musculus 55-58 1398925-4 1992 The SCID-PBL-Hu mice with high concentrations of human IgG regularly had mono- or oligoclonal human IgG bands in serum, as demonstrated by agarose gel electrophoresis. Sepharose 139-146 immunoglobulin heavy variable V1-62 Mus musculus 100-103 1382076-4 1992 Through affinity chromatography of membrane glycoproteins from chick brain on tenascin-Sepharose, we isolated a major cell surface ligand of 135 kD which we identified as contactin/F11 by NH2-terminal sequencing. Sepharose 87-96 tenascin C Gallus gallus 78-86 1331752-8 1992 Calf thymus DNA-Sepharose or DRE-Sepharose column chromatography showed that transformed human cytosolic AhR interacts with DNA as a single species. Sepharose 16-25 aryl hydrocarbon receptor Homo sapiens 105-108 1331752-8 1992 Calf thymus DNA-Sepharose or DRE-Sepharose column chromatography showed that transformed human cytosolic AhR interacts with DNA as a single species. Sepharose 33-42 aryl hydrocarbon receptor Homo sapiens 105-108 1363243-2 1992 NAG isoenzyme fractions in urine were measured by agarose electrophoresis-m-cresol sulfonphthaleinyl-NAG reaction. Sepharose 50-57 O-GlcNAcase Homo sapiens 0-3 16653083-7 1992 Pea mitochondrial Hsc70, purified by a combination of ATP-agarose affinity chromatography and gel permeation chromatography, was labeled when incubated with ATP plus calcium, suggesting autophosphorylation rather than phosphorylation by an associated kinase. Sepharose 58-65 heat shock protein family A (Hsp70) member 8 Homo sapiens 18-23 1458050-3 1992 Since chimeric B72.3 Fab" bound weakly to the protein G-Sepharose it could be separated from F(ab")2 and eluted with a pH 7 wash whereas B72.3 F(ab")2 required elution at pH 2. Sepharose 56-65 FA complementation group B Homo sapiens 21-24 1284390-2 1992 They formed opaque precipitation rings with IgA in agarose containing 2% PEG 6000. Sepharose 51-58 CD79a molecule Homo sapiens 44-47 1384470-1 1992 FKBP59-HBI, a heat shock protein hsp90-binding immunophilin that was originally detected in heterooligomer forms of steroid receptors, is retained on Calmodulin (CAM)-Sepharose 4B in the presence of 2 mM Ca2+ and is eluted by EGTA, demonstrating a specific p59-CAM interaction. Sepharose 167-176 peptidyl-prolyl cis-trans isomerase FKBP4 Oryctolagus cuniculus 0-6 1384470-1 1992 FKBP59-HBI, a heat shock protein hsp90-binding immunophilin that was originally detected in heterooligomer forms of steroid receptors, is retained on Calmodulin (CAM)-Sepharose 4B in the presence of 2 mM Ca2+ and is eluted by EGTA, demonstrating a specific p59-CAM interaction. Sepharose 167-176 peptidyl-prolyl cis-trans isomerase FKBP4 Oryctolagus cuniculus 7-10 1384470-1 1992 FKBP59-HBI, a heat shock protein hsp90-binding immunophilin that was originally detected in heterooligomer forms of steroid receptors, is retained on Calmodulin (CAM)-Sepharose 4B in the presence of 2 mM Ca2+ and is eluted by EGTA, demonstrating a specific p59-CAM interaction. Sepharose 167-176 calmodulin Oryctolagus cuniculus 150-160 1384470-1 1992 FKBP59-HBI, a heat shock protein hsp90-binding immunophilin that was originally detected in heterooligomer forms of steroid receptors, is retained on Calmodulin (CAM)-Sepharose 4B in the presence of 2 mM Ca2+ and is eluted by EGTA, demonstrating a specific p59-CAM interaction. Sepharose 167-176 calmodulin Oryctolagus cuniculus 162-165 1326560-2 1992 The cytochrome b558 was solubilized from membranes with the detergent n-heptyl-beta-thioglucoside and purified by DEAE-Sepharose and heparin-Sepharose chromatographies. Sepharose 119-128 cytochrome b Sus scrofa 4-16 1461569-1 1992 Differences in glycosylation of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) in human brain, plasma and cerebrospinal fluid (CSF) have been investigated by means of their interaction with agarose-immobilized lectins. Sepharose 205-212 acetylcholinesterase (Cartwright blood group) Homo sapiens 32-52 1461569-3 1992 Brain, plasma and CSF BuChE was almost fully bound to Con A, LCA and WGA-agarose. Sepharose 73-80 butyrylcholinesterase Homo sapiens 22-27 1327146-5 1992 Partial purification of neutrophil cytosolic PLC on Q Sepharose, phenyl Sepharose and heparin-Agarose columns is described. Sepharose 54-63 heparan sulfate proteoglycan 2 Homo sapiens 45-48 1530639-3 1992 The proteolysis of the alpha-chain of MHC class I was shown to take place between the alpha 2- and alpha 3- domains as estimated by the Con A-Sepharose precipitation pattern on SDS-PAGE. Sepharose 142-151 Fc gamma receptor and transporter Homo sapiens 23-34 1327146-6 1992 From heparin-Agarose column, two PLC activity peaks exhibiting different substrate specificities were eluted. Sepharose 13-20 heparan sulfate proteoglycan 2 Homo sapiens 33-36 1326533-3 1992 The membrane-associated cytochrome b558 was solubilized with a detergent, n-heptyl beta-thioglucoside, and purified by DEAE-Sepharose, heparin-Sepharose, and Mono Q column chromatography. Sepharose 124-133 cytochrome b Oryctolagus cuniculus 24-36 1326533-6 1992 However, the system showed high O2(-)-generating activity of 31.8 mol/s/mol of cytochrome b558 (52.5% of the original O2(-)-generating activity of the solubilized membranes) in the presence of a nitro blue tetrazolium (NBT) reductase fraction that was separated from the cytochrome b fraction by heparin-Sepharose chromatography. Sepharose 304-313 cytochrome b Oryctolagus cuniculus 79-91 1525179-3 1992 Here we describe a method for the one-step immunoaffinity purification of t-PA from conditioned melanoma cell medium, using 1-3-1 immobilised on Sepharose under mild elution conditions, favourable for t-PA. Sepharose 145-154 plasminogen activator, tissue type Homo sapiens 74-78 1527019-3 1992 The receptor was isolated by affinity chromatography on mouse recombinant entactin-Sepharose of 125I surface-labeled octyl glucoside cell extracts. Sepharose 83-92 nidogen 1 Mus musculus 74-82 1527019-4 1992 The receptor, which consisted of two polypeptides of relative molecular masses of 150 and 116 kDa, bound to the entactin-Sepharose matrix in the presence of CaCl2, MgCl2, and MnCl2, and was eluted with EDTA, but not with Arg-Gly-Asp-containing peptides. Sepharose 121-130 nidogen 1 Homo sapiens 112-120 1527019-6 1992 Purified alpha 3 beta 1 bound to entactin Sepharose in a divalent cation-dependent manner and liposomes prepared with fractions eluted from the entactin-Sepharose matrix, as well as purified alpha 3 beta 1 also bound to entactin. Sepharose 42-51 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 17-23 1527021-5 1992 A quantitative assay was developed which rapidly measured the ability of wild-type or mutant glutathione S-transferase to bind to glutathione-agarose. Sepharose 142-149 glutathione S-transferase kappa 1 Homo sapiens 93-118 1527025-2 1992 We have developed a significantly improved method for isolation of RK based on the specific interactions of phosphorylated forms of the enzyme with heparin-Sepharose. Sepharose 156-165 G protein-coupled receptor kinase 1 Homo sapiens 67-69 1528859-2 1992 The lipase was purified 100- to 250-fold from the supernatant of lipolytically quiet cells to 10-20% purity by a single passage over phenyl-Sepharose resin with high (greater than 70%) activity yields. Sepharose 140-149 lipase G, endothelial type Rattus norvegicus 4-10 1419789-8 1992 Inhibition of extracellular calmodulin activity by a calmodulin antagonist immobilized on agarose beads, or by an antibody to calmodulin significantly decreased proliferation in all dividing cultures. Sepharose 90-97 calmodulin 1 Homo sapiens 28-38 1358574-1 1992 Soluble CD4 (sT4) has been metabolically labeled with [3H]leucine in Chinese hamster ovary cells and purified by S Sepharose chromatography. Sepharose 115-124 T-cell surface glycoprotein CD4 Cricetulus griseus 8-11 1499862-4 1992 We obtained a specific antiproinsulin antibody by absorbing the initial goat antiserum with human C-peptide-agarose. Sepharose 108-115 insulin Homo sapiens 98-107 1380438-2 1992 Insulin-dependent tyrosine phosphorylation of a monomeric 195K glycoprotein (pp195) was observed in wheatgerm agglutinin (WGA)-Sepharose-purified insulin receptor preparations from rat liver and muscle. Sepharose 127-136 insulin receptor Rattus norvegicus 146-162 1355095-7 1992 For characterization of cytoskeletal proteins interacting with ICAM-1, a 28-residue peptide that encompasses the entire predicted cytoplasmic domain (ICAM-1,478-505) was synthesized, coupled to Sepharose-4B, and used as an affinity matrix. Sepharose 194-203 intercellular adhesion molecule 1 Homo sapiens 63-69 1355095-7 1992 For characterization of cytoskeletal proteins interacting with ICAM-1, a 28-residue peptide that encompasses the entire predicted cytoplasmic domain (ICAM-1,478-505) was synthesized, coupled to Sepharose-4B, and used as an affinity matrix. Sepharose 194-203 intercellular adhesion molecule 1 Homo sapiens 150-156 1402396-10 1992 In contrast, A-I/A-II-HDL2 were twice as effective as A-I-HDL2 in liberating hepatic lipase immobilized on HDL3-Sepharose. Sepharose 112-121 junctophilin 3 Homo sapiens 17-26 1402396-10 1992 In contrast, A-I/A-II-HDL2 were twice as effective as A-I-HDL2 in liberating hepatic lipase immobilized on HDL3-Sepharose. Sepharose 112-121 lipase C, hepatic type Homo sapiens 77-91 1402396-10 1992 In contrast, A-I/A-II-HDL2 were twice as effective as A-I-HDL2 in liberating hepatic lipase immobilized on HDL3-Sepharose. Sepharose 112-121 HDL3 Homo sapiens 107-111 1323818-8 1992 Furthermore, both v-Mos and the p35cdk co-precipitated with p13suc1-Sepharose beads. Sepharose 68-77 MOS proto-oncogene, serine/threonine kinase Homo sapiens 20-23 1337603-1 1992 Latent protein phosphatase, Fc.M, was purified from porcine heart extracts by a procedure involving precipitation at pH 5.0, DEAE-Sephacel chromatography, ammonium sulfate fractionation, chromatography on phenyl-Sepharose, Biogel-A 0.5m and poly-L-lysine-agarose. Sepharose 255-262 nucleolar protein 3 Homo sapiens 28-32 1434029-3 1992 TSA, which was detectable in all of 11 women in normal early pregnancy, correlated positively with serum hCG level, but was abolished completely by the pretreatment of serum samples with the solid-phase hCG antibody coupled with Sepharose-4B. Sepharose 229-238 hypertrichosis 2 (generalised, congenital) Homo sapiens 105-108 1281927-6 1992 P. carinii also bound to purified glycoproteins coupled to Sepharose or adsorbed to plastic, and the binding was inhibited by soluble bovine submaxillary mucin. Sepharose 59-68 mucin 1, cell surface associated Bos taurus 154-159 1359673-2 1992 After PCR amplification of the polymorphic second exon of the HLA-DPB1 locus, the PCR product was digested with seven allele-specific restriction endonucleases: RsaI, FokI, ApaI, SacI, BstUI, EcoNI, and DdeI, and the DNA fragments were separated by electrophoresis in agarose gels. Sepharose 268-275 major histocompatibility complex, class II, DP beta 1 Homo sapiens 62-70 1322114-2 1992 Incubation of the cytosol plus the different radioligands for 2 h at 20 degrees C resulted in the formation of Ah receptor complexes which exhibited increased retention times on DNA-Sepharose columns. Sepharose 182-191 aryl hydrocarbon receptor Rattus norvegicus 111-122 1511689-1 1992 Aminopeptidase H was purified from fresh chicken breast muscle by ammonium sulfate fractionation and successive chromatographies on DEAE-cellulose, Ultrogel AcA 34, activated thiol-Sepharose 4B, phenyl-Sepharose CL-4B and DEAE-cellulose again. Sepharose 181-190 bleomycin hydrolase Gallus gallus 0-16 1644832-2 1992 Previous studies from this laboratory using LPL-Sepharose affinity chromatography identified a 220-kDa LPL binding proteoglycan. Sepharose 48-57 lipoprotein lipase Homo sapiens 44-47 1644832-2 1992 Previous studies from this laboratory using LPL-Sepharose affinity chromatography identified a 220-kDa LPL binding proteoglycan. Sepharose 48-57 lipoprotein lipase Homo sapiens 103-106 1644820-9 1992 The combination of Bio-Rex 70-, DNA-Sepharose-, and sequence-specific DNA affinity column chromatography yielded a highly purified preparation of MGF. Sepharose 36-45 transforming growth factor beta 2 Bos taurus 146-149 1644832-6 1992 Furthermore, the biotinylated 116-kDa protein bound to LPL-Sepharose and eluted with 0.4 M NaCl suggesting that the 116-kDa LPL binding protein is present on the cell surface. Sepharose 59-68 lipoprotein lipase Homo sapiens 55-58 1644832-6 1992 Furthermore, the biotinylated 116-kDa protein bound to LPL-Sepharose and eluted with 0.4 M NaCl suggesting that the 116-kDa LPL binding protein is present on the cell surface. Sepharose 59-68 lipoprotein lipase Homo sapiens 124-127 1412220-5 1992 However, when thrombin was modified with PPACK after coupling to the agarose matrix, ability to bind radiolabel was lost. Sepharose 69-76 coagulation factor II, thrombin Homo sapiens 14-22 1644832-7 1992 When detergent extracts of endothelial cells were applied to LPL-Sepharose in the presence of 0.15 M NaCl, the 116-kDa, but not the 220-kDa, protein still bound to LPL-Sepharose. Sepharose 65-74 lipoprotein lipase Homo sapiens 61-64 1644832-7 1992 When detergent extracts of endothelial cells were applied to LPL-Sepharose in the presence of 0.15 M NaCl, the 116-kDa, but not the 220-kDa, protein still bound to LPL-Sepharose. Sepharose 168-177 lipoprotein lipase Homo sapiens 164-167 1644833-1 1992 In this report we describe the purification of the murine interleukin 3 receptor (mIL-3R) to apparent homogeneity using a two-step procedure involving biotinylated mIL-3 (B-mIL-3) and affinity binding to immobilized antiphosphotyrosine and streptavidin agarose (SA). Sepharose 253-260 colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage) Mus musculus 82-88 1644833-1 1992 In this report we describe the purification of the murine interleukin 3 receptor (mIL-3R) to apparent homogeneity using a two-step procedure involving biotinylated mIL-3 (B-mIL-3) and affinity binding to immobilized antiphosphotyrosine and streptavidin agarose (SA). Sepharose 253-260 interleukin 3 Mus musculus 82-87 1644833-2 1992 Purification was monitored using an assay for detergent solubilized-mIL-3Rs that utilized unglycosylated 125I-mIL-3 and concanavalin A (ConA)-Sepharose beads. Sepharose 142-151 interleukin 3 Mus musculus 68-73 1412223-4 1992 The modified antithrombin III retained a reduced affinity for heparin (eluting at 0.3M NaCl from heparin Agarose) and was observed to be a competitive inhibitor of the heparin-dependent rate of inhibition of thrombin by native antithrombin III. Sepharose 105-112 serpin family C member 1 Homo sapiens 13-29 1412223-4 1992 The modified antithrombin III retained a reduced affinity for heparin (eluting at 0.3M NaCl from heparin Agarose) and was observed to be a competitive inhibitor of the heparin-dependent rate of inhibition of thrombin by native antithrombin III. Sepharose 105-112 coagulation factor II, thrombin Homo sapiens 17-25 1642659-3 1992 METHODS: Anti-IL-6 IgG autoantibodies were measured by the 125I-IL-6 binding activity of IgG, which was isolated from serum by protein A-Sepharose. Sepharose 137-146 interleukin 6 Homo sapiens 14-18 1412165-5 1992 We prepared Lp(a) from plasma by sequential ultracentrifugation followed by lysine-sepharose affinity chromatography. Sepharose 83-92 lipoprotein(a) Homo sapiens 12-17 1525985-1 1992 To evaluate a method to quantitate the isoforms of serum creatine kinase isoenzyme MM (CK-MM) by agarose gel electrophoresis, sera of normal subjects (n = 74) and patients with acute myocardial infarction (n = 21) and other diseases (n = 67) were studied. Sepharose 97-104 creatine kinase, M-type Homo sapiens 57-85 1321590-1 1992 The NCI-H69 cell alpha 1----3fucosyltransferase has been purified from a 0.2% Triton X-100R solubilized enzyme fraction by GDP-hexanolamine-Sepharose affinity chromatography and Superose 12 gel filtration. Sepharose 140-149 fucosyltransferase 11 Homo sapiens 17-47 1639159-8 1992 The assay with the immune IgG1 and IgG2, separated through protein A-Sepharose affinity chromatography, showed that IgG1 retains most of this capacity. Sepharose 69-78 LOC105243590 Mus musculus 26-30 1639159-8 1992 The assay with the immune IgG1 and IgG2, separated through protein A-Sepharose affinity chromatography, showed that IgG1 retains most of this capacity. Sepharose 69-78 LOC105243590 Mus musculus 116-120 1422140-7 1992 We next isolated GP65 from human peripheral blood lymphocytes by a combination of chromatography on a cation-exchange column and a WGA-agarose column and preparative SDS gel electrophoresis. Sepharose 135-142 neuroplastin Homo sapiens 17-21 1505964-3 1992 Amplification by the polymerase chain reaction (PCR) of a genomic fragment encompassing GNAS1 exons 7 and 8 from one patient resulted in a product with aberrant migration on nondenaturing polyacrylamide and agarose gels. Sepharose 207-214 GNAS complex locus Homo sapiens 88-93 1525985-1 1992 To evaluate a method to quantitate the isoforms of serum creatine kinase isoenzyme MM (CK-MM) by agarose gel electrophoresis, sera of normal subjects (n = 74) and patients with acute myocardial infarction (n = 21) and other diseases (n = 67) were studied. Sepharose 97-104 creatine kinase, M-type Homo sapiens 87-92 1639873-9 1992 Synthetic peptides homologous to these three regions, but not to other regions of TGF-beta 1, were found to bind to heparin-Sepharose and were eluted with 0.15 M-0.30 M NaCl. Sepharose 124-133 transforming growth factor beta 1 Homo sapiens 82-92 1639872-8 1992 Thus, clusterin did not aggregate TM4 cells plated upon a laminin substratum, but readily aggregated TM4 cells plated upon an agarose-coated substratum, independent of the sequence of addition of cells and clusterin to the culture dish. Sepharose 126-133 clusterin Mus musculus 6-15 1378864-2 1992 When the same RA sera were adsorbed to beta 2m-Sepharose affinity columns, eluted material showed predominant IgM anti-Fc of IgG and anti-beta 2m reactivity. Sepharose 47-56 beta-2-microglobulin Homo sapiens 39-46 1639873-3 1992 The present studies indicate that 20-70% of iodinated TGF-beta 1 binds to heparin-Sepharose and the retained fraction is eluted with approximately 0.37 M NaCl. Sepharose 82-91 transforming growth factor beta 1 Homo sapiens 54-64 1639873-4 1992 Native, unlabelled platelet TGF-beta 1, however, is completely retained by heparin-Sepharose and eluted with 0.9-1.2 M NaCl. Sepharose 83-92 transforming growth factor beta 1 Homo sapiens 28-38 1279089-12 1992 The effect of conformation and dimeric structure on LPL-heparin interaction was studied by heparin-Sepharose chromatography. Sepharose 99-108 lipoprotein lipase Homo sapiens 52-55 1321340-2 1992 pp39mos is coprecipitated with p34cdc2 when using either anti-PSTAIR antibody or p13suc1-Sepharose beads. Sepharose 89-98 cyclin-dependent kinase 1 Mus musculus 31-38 1322379-1 1992 In a previous study on several independently established Epstein-Barr virus (EBV)-converted sublines of the EBV-negative Burkitt lymphoma (BL) line BL41, we found that expression of the virally encoded membrane protein LMP1 was accompanied by reduced agarose clonability and tumorigenicity. Sepharose 251-258 LMP1 Human gammaherpesvirus 4 219-223 1497654-4 1992 In contrast to Ca(2+)-binding protein calsequestrin not having detectable 65Zn-binding sites, HRC protein bound selectively to immobilized Zn2+ on IDA-agarose affinity columns. Sepharose 151-158 sarcoplasmic reticulum histidine-rich calcium-binding protein Oryctolagus cuniculus 94-97 1637820-1 1992 Human S-adenosylmethionine decarboxylase (AdoMetDC) was expressed in high yield in Escherichia coli using the pIN-III(lppP-5) expression vector and purified to apparent homogeneity using affinity chromatography on methylglyoxal bis(guanylhydrazone)-Sepharose. Sepharose 249-258 adenosylmethionine decarboxylase 1 Homo sapiens 6-40 1637820-1 1992 Human S-adenosylmethionine decarboxylase (AdoMetDC) was expressed in high yield in Escherichia coli using the pIN-III(lppP-5) expression vector and purified to apparent homogeneity using affinity chromatography on methylglyoxal bis(guanylhydrazone)-Sepharose. Sepharose 249-258 adenosylmethionine decarboxylase 1 Homo sapiens 42-50 1642648-1 1992 A highly active preparation of rat liver dihydrodiol/3 alpha-hydroxysteroid dehydrogenase was obtained using a newly developed, rapid purification scheme involving affinity chromatography on Red Sepharose. Sepharose 195-204 aldo-keto reductase family 1, member C14 Rattus norvegicus 53-89 1624806-3 1992 For example, eosinophils cultured for 7 days with hrIL-5 released 30 and 10% of granule eosinophil-derived neurotoxin (EDN) when exposed to Sepharose 4B beads coupled to secretory IgA and IgG, respectively, whereas freshly isolated eosinophils released only 19 and 4%, respectively, of their EDN in response to the same stimuli. Sepharose 140-152 ribonuclease A family member 2 Homo sapiens 88-117 1624806-3 1992 For example, eosinophils cultured for 7 days with hrIL-5 released 30 and 10% of granule eosinophil-derived neurotoxin (EDN) when exposed to Sepharose 4B beads coupled to secretory IgA and IgG, respectively, whereas freshly isolated eosinophils released only 19 and 4%, respectively, of their EDN in response to the same stimuli. Sepharose 140-152 ribonuclease A family member 2 Homo sapiens 119-122 1632469-3 1992 Solubilized plasma membranes of human cerebral cortical gray matter were subjected to affinity chromatography on alpha 2M-methylamine-sepharose. Sepharose 134-143 alpha-2-macroglobulin Homo sapiens 113-121 1445429-1 1992 A molecular form of CEA non-binding Con A (CEAnC) was isolated from colon adenocarcinoma metastases in liver as a fraction of CEA having no affinity to Con-A-Sepharose. Sepharose 158-167 CEA cell adhesion molecule 3 Homo sapiens 20-23 1377164-4 1992 A biochemical analysis of AFP on affinity sepharose columns revealed that the AFP derived from the tumor tissues was similar to that of hepatocellular carcinoma. Sepharose 42-51 alpha fetoprotein Homo sapiens 26-29 1382290-7 1992 The tyrosine-phosphorylated pp55 substrate appeared to be associated with EGFR; both pp55 and EGFR were adsorbed to EGF-Affi-Gel, wheat germ lectin-Sepharose, and anti-EGFR antibodies bound to protein A-Sepharose. Sepharose 148-157 epidermal growth factor receptor Mus musculus 94-98 1382290-7 1992 The tyrosine-phosphorylated pp55 substrate appeared to be associated with EGFR; both pp55 and EGFR were adsorbed to EGF-Affi-Gel, wheat germ lectin-Sepharose, and anti-EGFR antibodies bound to protein A-Sepharose. Sepharose 148-157 epidermal growth factor receptor Mus musculus 94-98 1382290-7 1992 The tyrosine-phosphorylated pp55 substrate appeared to be associated with EGFR; both pp55 and EGFR were adsorbed to EGF-Affi-Gel, wheat germ lectin-Sepharose, and anti-EGFR antibodies bound to protein A-Sepharose. Sepharose 203-212 epidermal growth factor receptor Mus musculus 94-98 1382290-7 1992 The tyrosine-phosphorylated pp55 substrate appeared to be associated with EGFR; both pp55 and EGFR were adsorbed to EGF-Affi-Gel, wheat germ lectin-Sepharose, and anti-EGFR antibodies bound to protein A-Sepharose. Sepharose 203-212 epidermal growth factor receptor Mus musculus 94-98 1320902-1 1992 Two receptors for tumor necrosis factor-alpha (TNF) were purified from detergent-solubilized human lung tissues by adsorption to TNF-Sepharose, followed by elution with low pH. Sepharose 133-142 tumor necrosis factor Homo sapiens 18-45 1320902-1 1992 Two receptors for tumor necrosis factor-alpha (TNF) were purified from detergent-solubilized human lung tissues by adsorption to TNF-Sepharose, followed by elution with low pH. Sepharose 133-142 tumor necrosis factor Homo sapiens 47-50 1320902-1 1992 Two receptors for tumor necrosis factor-alpha (TNF) were purified from detergent-solubilized human lung tissues by adsorption to TNF-Sepharose, followed by elution with low pH. Sepharose 133-142 tumor necrosis factor Homo sapiens 129-132 1320902-6 1992 Both the 75- and 55-kD receptors could be precipitated from 125I-surface-labeled or 35S-methionine-labeled U937 cells using TNF-Sepharose or anti-receptor antibodies. Sepharose 128-137 tumor necrosis factor Homo sapiens 124-127 1503565-1 1992 The mechanism of how chloroethylamine-treated agarose greatly simplifies the purification of ceruloplasmin (Cp) by preferentially binding this protein from blood plasma has been investigated. Sepharose 46-53 ceruloplasmin Homo sapiens 93-106 1394704-6 1992 As a result, it was found that beta-glucuronidase had an affinity for adsorbents prepared with either acetyl derivatives or methoxy derivatives of glycosides and CH-Sepharose 4B. Sepharose 165-174 beta-glucuronidase Bos taurus 31-49 1499287-2 1992 Cathepsin H was purified about 5400-fold from hepatopancreas of carp (Cyprinus carpio) by the method involving ammonium sulfate fractionation, and chromatography on S-Sepharose, DEAE-Sephacel, Ultrogel AcA54, Concanavalin A-Sepharose 4B and GPC on Protein-Pak 125. Sepharose 167-176 cathepsin H Rattus norvegicus 0-11 1499287-2 1992 Cathepsin H was purified about 5400-fold from hepatopancreas of carp (Cyprinus carpio) by the method involving ammonium sulfate fractionation, and chromatography on S-Sepharose, DEAE-Sephacel, Ultrogel AcA54, Concanavalin A-Sepharose 4B and GPC on Protein-Pak 125. Sepharose 224-233 cathepsin H Rattus norvegicus 0-11 1378864-6 1992 Conversely, RF eluted from beta 2m Sepharose reacted slightly more with beta 2 m than Fc. Sepharose 35-44 beta-2-microglobulin Homo sapiens 27-34 1339284-4 1992 For structural studies, human SP-D was isolated from amniotic fluid by affinity chromatography on maltose-Sepharose followed by f.p.l.c. Sepharose 106-115 surfactant protein D Homo sapiens 30-34 1377216-5 1992 We found evidence for the formation of a high-affinity 1:1 complex between SAP and heparin and for inhibition of binding of both thrombin and antithrombin III to heparin-Sepharose by SAP. Sepharose 170-179 amyloid P component, serum Homo sapiens 75-78 1377216-5 1992 We found evidence for the formation of a high-affinity 1:1 complex between SAP and heparin and for inhibition of binding of both thrombin and antithrombin III to heparin-Sepharose by SAP. Sepharose 170-179 coagulation factor II, thrombin Homo sapiens 129-137 1377216-5 1992 We found evidence for the formation of a high-affinity 1:1 complex between SAP and heparin and for inhibition of binding of both thrombin and antithrombin III to heparin-Sepharose by SAP. Sepharose 170-179 serpin family C member 1 Homo sapiens 142-158 1377216-5 1992 We found evidence for the formation of a high-affinity 1:1 complex between SAP and heparin and for inhibition of binding of both thrombin and antithrombin III to heparin-Sepharose by SAP. Sepharose 170-179 amyloid P component, serum Homo sapiens 183-186 1380928-13 1992 Two types of peptide epitopes seemed to be present in rhCG beta since when the flowthrough fraction from the ovalbumin-glycopeptide-affinity column was passed through the hCG beta-Sepharose column, the antibodies in the flowthrough from the latter column were specific to the unique antigenic determinants present only in the rhCG beta and not in hCG beta. Sepharose 180-189 Rh family C glycoprotein Homo sapiens 54-58 1380928-13 1992 Two types of peptide epitopes seemed to be present in rhCG beta since when the flowthrough fraction from the ovalbumin-glycopeptide-affinity column was passed through the hCG beta-Sepharose column, the antibodies in the flowthrough from the latter column were specific to the unique antigenic determinants present only in the rhCG beta and not in hCG beta. Sepharose 180-189 chorionic gonadotropin subunit beta 3 Homo sapiens 55-63 1380928-14 1992 The eluate from the hCG beta-Sepharose column contained the third type of antibodies, being the predominant ones, directed to the common epitopes between rhCG beta and hCG beta. Sepharose 29-38 chorionic gonadotropin subunit beta 3 Homo sapiens 20-28 1380928-14 1992 The eluate from the hCG beta-Sepharose column contained the third type of antibodies, being the predominant ones, directed to the common epitopes between rhCG beta and hCG beta. Sepharose 29-38 Rh family C glycoprotein Homo sapiens 154-158 1380928-14 1992 The eluate from the hCG beta-Sepharose column contained the third type of antibodies, being the predominant ones, directed to the common epitopes between rhCG beta and hCG beta. Sepharose 29-38 chorionic gonadotropin subunit beta 3 Homo sapiens 155-163 1377399-4 1992 By radioimmunoassay (RIA) based on monoclonal and polyclonal S-protein antibodies, we detected S-protein on harvested agarose beads. Sepharose 118-125 vitronectin Homo sapiens 95-104 1377399-6 1992 We also found a strong binding of S-protein antibodies to agarose beads preincubated in native serum, which was strongly reduced (70-80%) by inactivation of the alternative complement pathway (50 degrees C, 20 min). Sepharose 58-65 vitronectin Homo sapiens 34-43 1632822-0 1992 Immobilization of solubilized UDP-glucuronosyltransferase from rat liver microsomes to Sepharose 4B. Sepharose 87-99 UDP glycosyltransferase 2 family, polypeptide B Rattus norvegicus 30-57 1632822-1 1992 A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. Sepharose 107-114 UDP glycosyltransferase 2 family, polypeptide B Rattus norvegicus 47-74 1639919-1 1992 Aminophenylboronate-substituted agarose in 20 mM N-2-hydroxyethylpiperazine-N"-2-ethanesulphonic acid, pH 8.5, selectively adsorbs immunoglobulins and complement factors C3 and C4 from human serum. Sepharose 32-39 complement C3 Homo sapiens 170-179 1639922-4 1992 Divinylsulfone-activated agarose (6%) was used to immobilize three different synthetic peptides representing metal-binding protein surface domains from the human plasma metal transport protein histidine-rich glycoprotein (HRG). Sepharose 25-32 histidine rich glycoprotein Homo sapiens 222-225 1613466-3 1992 Although these proteins copurified with MCP-1 and IL-8 on heparin-Sepharose, they could be separated by cation-exchange fast protein liquid chromatography and reverse-phase high-performance liquid chromatography. Sepharose 66-75 C-X-C motif chemokine ligand 8 Homo sapiens 50-54 1325221-7 1992 Western blotting analysis with antibodies for the cdc2-encoded protein kinase and p13suc1-agarose binding studies allowed positive identification of this MBP kinase as p34cdc2. Sepharose 90-97 myelin basic protein Gallus gallus 154-157 1380142-2 1992 A major peak of mitogenic activity (heparin binding growth factor 2, HBGF-2) eluted from heparin-Sepharose between 1.7 and 2.0 M NaCl. Sepharose 97-106 fibroblast growth factor 2 Monodelphis domestica 36-67 1380142-2 1992 A major peak of mitogenic activity (heparin binding growth factor 2, HBGF-2) eluted from heparin-Sepharose between 1.7 and 2.0 M NaCl. Sepharose 97-106 fibroblast growth factor 2 Monodelphis domestica 69-75 1380142-4 1992 Opossum brain heparin binding growth factor 1 (HBGF-1), a minor peak of activity, eluted from heparin-Sepharose at 1.1 NaCl and contained a 16.2 kDa protein that cross-reacted with antiserum against bovine aFGF. Sepharose 102-111 fibroblast growth factor 1 Bos taurus 14-45 1380142-4 1992 Opossum brain heparin binding growth factor 1 (HBGF-1), a minor peak of activity, eluted from heparin-Sepharose at 1.1 NaCl and contained a 16.2 kDa protein that cross-reacted with antiserum against bovine aFGF. Sepharose 102-111 fibroblast growth factor 1 Bos taurus 47-53 1608942-5 1992 A c-Jun kinase activity was affinity-purified 5000-fold by using glutathione S-transferase-c-Jun-glutathione-Sepharose beads and was found to phosphorylate the N terminus of c-Jun but not v-Jun or c-Jun containing a 27-amino acid N-terminal deletion found in v-Jun. Sepharose 109-118 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 2-7 1614866-6 1992 The limit of detection of dsDNA-TOTO and YOYO complexes with a laser-excited confocal fluorescence gel scanner for a band 5-mm wide on a 1-mm thick agarose gel was 4 picograms, about 500-fold lower than attainable by conventional staining with ethidium bromide. Sepharose 148-155 HOP homeobox Homo sapiens 32-36 1601896-4 1992 Antibodies to selected inhibitory peptides affinity purified on a factor Xa-agarose column inhibited thrombin formation in a dose-dependent manner, indicating that the corresponding regions on factor Xa are surface-exposed. Sepharose 76-83 coagulation factor X Homo sapiens 66-75 1601896-4 1992 Antibodies to selected inhibitory peptides affinity purified on a factor Xa-agarose column inhibited thrombin formation in a dose-dependent manner, indicating that the corresponding regions on factor Xa are surface-exposed. Sepharose 76-83 coagulation factor II, thrombin Homo sapiens 101-109 1601896-4 1992 Antibodies to selected inhibitory peptides affinity purified on a factor Xa-agarose column inhibited thrombin formation in a dose-dependent manner, indicating that the corresponding regions on factor Xa are surface-exposed. Sepharose 76-83 coagulation factor X Homo sapiens 193-202 1608942-5 1992 A c-Jun kinase activity was affinity-purified 5000-fold by using glutathione S-transferase-c-Jun-glutathione-Sepharose beads and was found to phosphorylate the N terminus of c-Jun but not v-Jun or c-Jun containing a 27-amino acid N-terminal deletion found in v-Jun. Sepharose 109-118 glutathione S-transferase kappa 1 Homo sapiens 65-90 1608942-5 1992 A c-Jun kinase activity was affinity-purified 5000-fold by using glutathione S-transferase-c-Jun-glutathione-Sepharose beads and was found to phosphorylate the N terminus of c-Jun but not v-Jun or c-Jun containing a 27-amino acid N-terminal deletion found in v-Jun. Sepharose 109-118 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 91-96 1317864-3 1992 Solubilized IGF-I receptor was immobilized on antireceptor antibody-agarose beads. Sepharose 68-75 insulin like growth factor 1 receptor Homo sapiens 12-26 1608942-5 1992 A c-Jun kinase activity was affinity-purified 5000-fold by using glutathione S-transferase-c-Jun-glutathione-Sepharose beads and was found to phosphorylate the N terminus of c-Jun but not v-Jun or c-Jun containing a 27-amino acid N-terminal deletion found in v-Jun. Sepharose 109-118 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 91-96 1608942-5 1992 A c-Jun kinase activity was affinity-purified 5000-fold by using glutathione S-transferase-c-Jun-glutathione-Sepharose beads and was found to phosphorylate the N terminus of c-Jun but not v-Jun or c-Jun containing a 27-amino acid N-terminal deletion found in v-Jun. Sepharose 109-118 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 91-96 1385104-3 1992 Inactive renin was converted into active renin with Sepharose-bound trypsin. Sepharose 52-61 renin Homo sapiens 9-14 1375119-5 1992 Partial reduction of complement functional activity was shown when serum was absorbed by an HRG-Sepharose 6MB column. Sepharose 96-105 histidine-rich glycoprotein Ovis aries 92-95 1317779-5 1992 Gel permeation chromatography of the CIT-agarose eluates revealed one protein peak that coincided with PDE activity at an elution position of 135,000 daltons. Sepharose 41-48 phosphodiesterase 3A Homo sapiens 103-106 1375940-3 1992 Here we show that purified brain NO synthase binds to cytochrome c-agarose and exhibits superoxide dismutase-insensitive cytochrome c reductase activity with a Vmax of 10.2 mumol x mg-1 x min-1 and a Km of 34.1 microM. Sepharose 67-74 cytochrome c, somatic Homo sapiens 54-66 1394685-2 1992 Highly purified cathepsin G was obtained from column of EAH Sepharose 4B or Suc-L-Tyr-D-Leu-D-Val-pNA-Sepharose (affinity chromatography) by elution with poly-L-lysine solution (0.4 mg/ml, molecular weight (MW.) Sepharose 60-72 cathepsin G Homo sapiens 16-27 1394685-2 1992 Highly purified cathepsin G was obtained from column of EAH Sepharose 4B or Suc-L-Tyr-D-Leu-D-Val-pNA-Sepharose (affinity chromatography) by elution with poly-L-lysine solution (0.4 mg/ml, molecular weight (MW.) Sepharose 60-69 cathepsin G Homo sapiens 16-27 1394685-5 1992 Leukocyte elastase, adsorbed to Suc-L-Tyr-D-Leu-D-Val-pNA-Sepharose, was not eluted with poly-L-lysine solution. Sepharose 58-67 elastase, neutrophil expressed Homo sapiens 0-18 1385104-3 1992 Inactive renin was converted into active renin with Sepharose-bound trypsin. Sepharose 52-61 renin Homo sapiens 41-46 1572393-7 1992 Affinity chromatography experiments revealed that the Mac-1 and p150,95 integrins could be isolated from monocyte-differentiated HL-60 cells or neutrophils on a denatured protein-Sepharose column. Sepharose 179-188 integrin subunit beta 2 Homo sapiens 54-59 1572393-7 1992 Affinity chromatography experiments revealed that the Mac-1 and p150,95 integrins could be isolated from monocyte-differentiated HL-60 cells or neutrophils on a denatured protein-Sepharose column. Sepharose 179-188 chromatin assembly factor 1 subunit A Homo sapiens 64-68 1323564-3 1992 When the preformed complexes of receptor (solubilized from canine brain and lung membranes) and biotinylated ET-1 were subjected to avidin agarose column chromatography, most of the ETA activity was recovered in the pass-through fraction and the remainder was recovered in the 0.5 M KSCN eluate as ligand-free forms. Sepharose 139-146 endothelin 1 Canis lupus familiaris 109-113 1323564-3 1992 When the preformed complexes of receptor (solubilized from canine brain and lung membranes) and biotinylated ET-1 were subjected to avidin agarose column chromatography, most of the ETA activity was recovered in the pass-through fraction and the remainder was recovered in the 0.5 M KSCN eluate as ligand-free forms. Sepharose 139-146 endothelin receptor type A Canis lupus familiaris 182-185 1323564-4 1992 On the other hand, the ETB activity bound firmly to the avidin agarose column was eluted with 1.5 M KSCN. Sepharose 63-70 endothelin receptor type B Canis lupus familiaris 23-26 1602739-9 1992 By a combination of acetone fractionation, affinity chromatography on Cm-papain-Sepharose, and gel exclusion chromatography a protein of approximately 14 kilodaltons was isolated from TT cells that reacted with antibodies against human cystatin C, and strongly inhibited papain. Sepharose 80-89 cystatin C Homo sapiens 236-246 1382725-6 1992 Studies on mucin polymer assembly revealed that mucin preparations prepared by equilibrium density gradient centrifugation and Sepharose 2B chromatography (Mantle, M., Mantle, D., and Allen, A. Sepharose 127-136 LOC100508689 Homo sapiens 48-53 1434041-2 1992 Fibronectin was purified from blood bank homologous plasma by gelatin-Sepharose 4B affinity chromatography. Sepharose 70-82 fibronectin 1 Homo sapiens 0-11 1640050-6 1992 The TfR was affinity purified from Lec8 cells metabolically radiolabeled with [3H]glucosamine and the receptor was found to bind tightly to HPA-Sepharose. Sepharose 144-153 transferrin receptor protein 1 Cricetulus griseus 4-7 1632075-2 1992 A vWf-Ag antiserum was raised in rabbits and purified by preabsorption with the low molecular weight vWf-Ag-deficient fraction of canine cryoprecipitate, followed by affinity chromatography on protein-A Sepharose. Sepharose 203-212 von Willebrand factor Canis lupus familiaris 2-5 16668941-5 1992 Recombinant cytochrome c reductase was purified by immunoaffinity chromatography on monoclonal antibody Zm2(69) Sepharose. Sepharose 112-121 cytochrome c Zea mays 12-24 1319206-4 1992 Affinity chromatography of heparin in cytochrome c immobilized to thiol-Sepharose shows that commercial heparin is eluted in the low-affinity and high-affinity fractions. Sepharose 72-81 cytochrome c, somatic Homo sapiens 38-50 1587861-10 1992 Here we provide direct evidence for recognition by AP-2 of isolated terminal domains immobilized on Sepharose and show that the core of the AP-2 molecule is responsible for this interaction. Sepharose 100-109 transcription factor AP-2 alpha Homo sapiens 51-55 1587861-10 1992 Here we provide direct evidence for recognition by AP-2 of isolated terminal domains immobilized on Sepharose and show that the core of the AP-2 molecule is responsible for this interaction. Sepharose 100-109 transcription factor AP-2 alpha Homo sapiens 140-144 1591243-4 1992 The c-fes cDNA was expressed at high levels in a baculovirus system, and the catalytically active recombinant c-fes gene product p93c-fes was partially purified by DEAE-Sepharose and tyrosine-agarose chromatography. Sepharose 192-199 FES proto-oncogene, tyrosine kinase Homo sapiens 129-137 1350246-1 1992 The bovine dopamine D2 receptor was purified by wheat-germ-agglutinin-Sepharose chromatography and affinity chromatography, using the D2-receptor-specific agonist N-0434. Sepharose 70-79 dopamine receptor D2 Bos taurus 11-31 1376110-10 1992 A fragment of 68 kDa, obtained by further digestion of the 140 kDa fragment, bound to the GPIIIb-Sepharose affinity column. Sepharose 97-106 CD36 molecule Homo sapiens 90-96 1577805-1 1992 A novel lysosomal alpha-mannosidase, with unique substrate specificity, has been partially purified from human spleen by chromatography through concanavalin A-Sepharose, DEAE-Sephadex, and Sephacryl S-300. Sepharose 159-168 mannosidase alpha class 2B member 1 Homo sapiens 8-35 1584769-14 1992 (vi) CBD covalently linked to crosslinked agarose beads inhibited interleukin 2 production by phorbol ester-stimulated EL-4 cells. Sepharose 42-49 interleukin 2 Mus musculus 66-79 1374404-5 1992 We find that FKBP12 and the RyRec are tightly associated in skeletal muscle SR on the basis of: 1) co-purification through sequential heparin-agarose, hydroxylapatite, and size exclusion chromatography columns; 2) coimmunoprecipitation of the RyRec and FKBP12 with anti-FKBP12 antibodies; and 3) subcellular localization of both proteins to the terminal cisternae of the SR, and not in the longitudinal tubules of SR, in fast twitch skeletal muscle. Sepharose 142-149 FKBP prolyl isomerase 1A Homo sapiens 13-19 1317705-3 1992 The enzyme was purified 3000-fold using phosphocellulose and calmodulin-Agarose column chromatography. Sepharose 72-79 calmodulin 1 Rattus norvegicus 61-71 1577755-2 1992 This hsp70, isolated by affinity chromatography with RNase S-peptide-Sepharose, is referred to as the 73-kDa peptide recognition protein (prp73). Sepharose 69-78 heat shock protein family A (Hsp70) member 4 Homo sapiens 5-10 1590376-7 1992 Decavanadate displaced LPL bound to heparin-Sepharose and increased LPL release into the perfusate of hearts. Sepharose 44-53 lipoprotein lipase Rattus norvegicus 23-26 1590758-1 1992 The interactions between guanine nucleotide regulatory proteins and the Ca(2+)-binding protein calmodulin were studied using calmodulin-Sepharose affinity chromatography. Sepharose 136-145 calmodulin Bos taurus 95-105 1590758-1 1992 The interactions between guanine nucleotide regulatory proteins and the Ca(2+)-binding protein calmodulin were studied using calmodulin-Sepharose affinity chromatography. Sepharose 136-145 calmodulin Bos taurus 125-135 1590758-2 1992 Purified bovine brain beta gamma subunits bound to calmodulin-Sepharose in a Ca(2+)-dependent manner. Sepharose 62-71 calmodulin Bos taurus 51-61 1590758-6 1992 In contrast to bovine brain G proteins, both purified transducin beta gamma subunits and beta gamma released from rhodopsin-activated transducin bound to calmodulin-Sepharose in a Ca(2+)-dependent manner. Sepharose 165-174 rhodopsin Bos taurus 114-123 1590758-6 1992 In contrast to bovine brain G proteins, both purified transducin beta gamma subunits and beta gamma released from rhodopsin-activated transducin bound to calmodulin-Sepharose in a Ca(2+)-dependent manner. Sepharose 165-174 calmodulin Bos taurus 154-164 18600909-6 1992 Thus, inner mitochondrial membranes bound to ConA-Sepharose appear to be a potentially interesting model for the study of immobilized multienzymatic complexes. Sepharose 50-59 CONA Bos taurus 45-49 1637867-5 1992 Chromatography of eIF-2 over ATP-agarose, in denaturing conditions that induce polypeptide subunit dissociation, results in selective retention of the beta-subunit of eIF-2. Sepharose 33-40 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 18-23 1637867-5 1992 Chromatography of eIF-2 over ATP-agarose, in denaturing conditions that induce polypeptide subunit dissociation, results in selective retention of the beta-subunit of eIF-2. Sepharose 33-40 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 167-172 1601772-6 1992 Depleting macro CM of ET-1 by affinity columns constructed with protein A agarose and anti-ET-1 antibody removed the contractile activity for coronary artery rings and tracheal smooth muscle strips. Sepharose 74-81 endothelin-1 Ovis aries 22-26 1602240-7 1992 Two binding proteins of approximately 35 and 80 kd were isolated from the surface of the peritoneal macrophages by affinity chromatography on a CEA-Sepharose column. Sepharose 148-157 CEA cell adhesion molecule 20 Rattus norvegicus 144-147 1639763-3 1992 One peptide was released after proteolysis of the native GDH by trypsin and purified by anhydrotrypsin agarose and reversed-phase HPLC. Sepharose 103-110 glutamate dehydrogenase 1 Homo sapiens 57-60 1583109-7 1992 By a modification of the published method for purification of the foreign polypeptide from the GST carrier, the recombinant P22 was readily purified to homogeneity by thrombin cleavage of the fusion protein while it was adsorbed to glutathione agarose. Sepharose 244-251 calcineurin like EF-hand protein 1 Homo sapiens 124-127 1588920-4 1992 Human lung microsomes contain approximately 10 pmol of P-450/mg of protein, on the basis of Fe2+.CO versus Fe2+ difference spectra of the eluates obtained from an octylamino-agarose column. Sepharose 174-181 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 55-60 1588920-6 1992 After DEAE-cellulose column chromatography, a partially purified P-450 fraction containing polypeptides of Mr 52,000 and 58,000 was obtained from the early fraction of the octylamino-agarose column eluate, and an electrophoretically homogeneous protein having a molecular weight of approximately 52,000 was recovered from a latter fraction. Sepharose 183-190 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 65-70 1588920-8 1992 The protein from the latter octylamino-agarose fraction was immunoreactive with anti-rat P-450 1A2 and anti-human P-450 1A2 but not with antibodies raised against other P-450 enzymes or autoimmune antibodies that specifically recognize human P-450 1A2. Sepharose 39-46 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 89-94 1588920-8 1992 The protein from the latter octylamino-agarose fraction was immunoreactive with anti-rat P-450 1A2 and anti-human P-450 1A2 but not with antibodies raised against other P-450 enzymes or autoimmune antibodies that specifically recognize human P-450 1A2. Sepharose 39-46 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 114-119 1588920-8 1992 The protein from the latter octylamino-agarose fraction was immunoreactive with anti-rat P-450 1A2 and anti-human P-450 1A2 but not with antibodies raised against other P-450 enzymes or autoimmune antibodies that specifically recognize human P-450 1A2. Sepharose 39-46 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 114-119 1588920-8 1992 The protein from the latter octylamino-agarose fraction was immunoreactive with anti-rat P-450 1A2 and anti-human P-450 1A2 but not with antibodies raised against other P-450 enzymes or autoimmune antibodies that specifically recognize human P-450 1A2. Sepharose 39-46 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 114-119 1575761-4 1992 The expressed hybrid molecule bound to the TTR-Sepharose. Sepharose 47-56 transthyretin Homo sapiens 43-46 1477659-3 1992 We amplified eight coding exons of the p53 gene using rat liver DNA as template, and, in each case, one major amplification product was apparent on agarose gels. Sepharose 148-155 Wistar clone pR53P1 p53 pseudogene Rattus norvegicus 39-42 1413649-1 1992 Highly purified preparation of prolactin with molecular mass 23 kDa, which is the main form of the hormone in human amnionic fluid, was isolated from the fluid using gel filtration on Sephadex G-100, chromatography on DEAE-Sepharose, concanavalin A Sepharose, CM-cellulose. Sepharose 223-232 prolactin Homo sapiens 31-40 1517328-3 1992 The artificial IgG-binding proteins [pA(AB)1-6], which had been expressed in Escherichia coli JM109, were purified by methods involving IgG-Sepharose affinity chromatography. Sepharose 140-149 NBPF member 10 Homo sapiens 37-46 1517328-4 1992 Among pA(AB)1-6 immobilized on cyanogen bromide-Sepharose, pA(AB)4-Sepharose was the highest in IgG-binding capacity at the same level of mg protein per ml gel, about 30% higher than protein A-Sepharose. Sepharose 48-57 NBPF member 10 Homo sapiens 6-15 1517328-4 1992 Among pA(AB)1-6 immobilized on cyanogen bromide-Sepharose, pA(AB)4-Sepharose was the highest in IgG-binding capacity at the same level of mg protein per ml gel, about 30% higher than protein A-Sepharose. Sepharose 67-76 NBPF member 10 Homo sapiens 6-15 1517347-3 1992 Their application is then illustrated by consideration of results from recycling partition equilibrium studies of the heparin-facilitated desorption of thrombin from heparin-Sepharose, and of the competition between methyl-alpha-D-mannoside and p-nitrophenyl-alpha-D- mannoside for concanavalin A immobilized on CPG-170. Sepharose 174-183 prothrombin Oryctolagus cuniculus 152-160 1517349-1 1992 The three proteins phosphorylase b, calmodulin and fibrinogen are adsorbed onto thioalkyl derivatives of Sepharose much more strongly than onto gels carrying the same alkyl residue coupled via a carbamate linkage. Sepharose 105-114 calmodulin 1 Homo sapiens 36-46 1517349-1 1992 The three proteins phosphorylase b, calmodulin and fibrinogen are adsorbed onto thioalkyl derivatives of Sepharose much more strongly than onto gels carrying the same alkyl residue coupled via a carbamate linkage. Sepharose 105-114 fibrinogen beta chain Homo sapiens 51-61 1583313-4 1992 This assay was used to evaluate SpA leakage when purifying a serum-free murine IgG1 cell culture supernatant using SpA immobilized on agarose (Protein A-Sepharose CL-4B) or controlled pore glass (Prosep A, high capacity). Sepharose 134-141 surfactant associated protein A1 Mus musculus 32-35 1374026-14 1992 Purification of the two mouse u-PAR variant proteins by diisopropylfluorophosphate-inactivated mouse u-PA-Sepharose affinity chromatography yielded two silver-stained bands when analysed by SDS/PAGE, corresponding in electrophoretic mobility to those seen by ligand-blotting analysis. Sepharose 106-115 plasminogen activator, urokinase receptor Mus musculus 30-35 1314597-1 1992 Dissociation of the complex of transcortin receptor with immobilized transcortin in the presence of 10(-5) M testosterone has been shown with the use of affinity chromatography on transcortin-Sepharose. Sepharose 192-201 serpin family A member 6 Homo sapiens 31-42 1314597-1 1992 Dissociation of the complex of transcortin receptor with immobilized transcortin in the presence of 10(-5) M testosterone has been shown with the use of affinity chromatography on transcortin-Sepharose. Sepharose 192-201 serpin family A member 6 Homo sapiens 69-80 1314597-1 1992 Dissociation of the complex of transcortin receptor with immobilized transcortin in the presence of 10(-5) M testosterone has been shown with the use of affinity chromatography on transcortin-Sepharose. Sepharose 192-201 serpin family A member 6 Homo sapiens 69-80 1532796-14 1992 Biotinated ATP11 protein can be partially purified by affinity chromatography on monomeric or tetrameric avidin coupled to Sepharose. Sepharose 123-132 Atp11p Saccharomyces cerevisiae S288C 11-16 1374026-14 1992 Purification of the two mouse u-PAR variant proteins by diisopropylfluorophosphate-inactivated mouse u-PA-Sepharose affinity chromatography yielded two silver-stained bands when analysed by SDS/PAGE, corresponding in electrophoretic mobility to those seen by ligand-blotting analysis. Sepharose 106-115 plasminogen activator, urokinase Mus musculus 30-34 1562726-3 1992 Wild-type recombinant TFPI (rTFPI), expressed in mouse C127 cells, separates into two forms on heparin-agarose chromatography that elute at 0.3 mol/L and 0.6 mol/L NaCl. Sepharose 103-110 tissue factor pathway inhibitor Mus musculus 22-26 1562726-10 1992 This form of rTFPI elutes from heparin-agarose at 0.28 mol/L NaCl and inhibits factor Xa at a rate that is slower than rTFPI(0.3). Sepharose 39-46 tissue factor pathway inhibitor Rattus norvegicus 13-18 1562726-3 1992 Wild-type recombinant TFPI (rTFPI), expressed in mouse C127 cells, separates into two forms on heparin-agarose chromatography that elute at 0.3 mol/L and 0.6 mol/L NaCl. Sepharose 103-110 tissue factor pathway inhibitor Rattus norvegicus 28-33 1313007-1 1992 The effective resolution of human platelet cytosolic phosphoinositide-phospholipase C (PLC) revealed five distinct activity peaks by Q-Sepharose and heparin-Sepharose column chromatographies when assayed using phosphatidylinositol (PI) and phosphatidylinositol 4,5-bisphosphate (PIP2). Sepharose 135-144 phospholipase C gamma 1 Homo sapiens 53-85 1313007-1 1992 The effective resolution of human platelet cytosolic phosphoinositide-phospholipase C (PLC) revealed five distinct activity peaks by Q-Sepharose and heparin-Sepharose column chromatographies when assayed using phosphatidylinositol (PI) and phosphatidylinositol 4,5-bisphosphate (PIP2). Sepharose 135-144 phospholipase C gamma 1 Homo sapiens 87-90 1551903-3 1992 Determination of the distribution of apoE among media lipoproteins by agarose column chromatography showed that the majority of secreted apoE was associated with large lipoproteins when cells were incubated with fetal bovine serum. Sepharose 70-77 apolipoprotein E Homo sapiens 37-41 1551911-3 1992 The protein kinase was separable from HSP90 by adsorption to heparin-Sepharose or phosphocellulose. Sepharose 69-78 heat shock protein 90 alpha family class A member 1 Homo sapiens 38-43 1590750-2 1992 ADH and ALDH isozyme phenotypes from 39 surgical Chinese lung specimens were identified by agarose isoelectric focusing. Sepharose 91-98 aldo-keto reductase family 1 member A1 Homo sapiens 0-3 1522178-4 1992 Synaptophysin was finally purified by preparative sodium dodecyl sulphate-polyacrylamine gel electrophoresis and synapsin I by affinity chromatography using a calmodulin-Sepharose column. Sepharose 170-179 synaptophysin Bos taurus 0-13 1581233-2 1992 The circulating highest molecular weight multimers (HMWM) of von Willebrand factor (vWF) were decreased when assessed by SDS-agarose plasma electrophoresis, leading to the diagnosis of type II AvWD. Sepharose 125-132 von Willebrand factor Homo sapiens 61-82 1581233-2 1992 The circulating highest molecular weight multimers (HMWM) of von Willebrand factor (vWF) were decreased when assessed by SDS-agarose plasma electrophoresis, leading to the diagnosis of type II AvWD. Sepharose 125-132 von Willebrand factor Homo sapiens 84-87 1516732-4 1992 Cathepsin A was purified homogeneously from porcine spleen by DE 52 column, Sephadex G-150 column and Try-Phe-CH-Sepharose column chromatography. Sepharose 113-122 cathepsin A Homo sapiens 0-11 1555592-1 1992 Affinity chromatography of human plasma on placental-alkaline-phosphatase-Sepharose columns (placental alkaline phosphatase, PLAP) yielded consistently a pure protein which was identified as IgG on the basis of electrophoretical and immunological comparisons with authentic human IgG. Sepharose 74-83 alkaline phosphatase, placental Homo sapiens 125-129 1569919-7 1992 DHEA-ST activity was purified from the 100,000 x g supernatant fraction of human adrenal tissue by DEAE-Sepharose CL-6B chromatography and 3",5"-diphosphoadenosine-agarose affinity chromatography. Sepharose 164-171 sulfotransferase family 2A member 1 Homo sapiens 0-7 1569928-15 1992 Direct interaction of polyanions with AT1 was suggested by the capacity of solubilized photoaffinity-labeled 125I-AT1 to adsorb to heparin-agarose gels. Sepharose 139-146 angiotensin II receptor type 1 Bos taurus 38-41 1569928-15 1992 Direct interaction of polyanions with AT1 was suggested by the capacity of solubilized photoaffinity-labeled 125I-AT1 to adsorb to heparin-agarose gels. Sepharose 139-146 angiotensin II receptor type 1 Bos taurus 114-117 1569928-16 1992 The adsorption of 125I-AT1 to heparin-agarose was inhibited by prior incubation of solubilized receptor with heparin or PVS. Sepharose 38-45 angiotensin II receptor type 1 Bos taurus 23-26 1603034-6 1992 Decorin is also synthesized in small amounts but it is rapidly lost from the agarose or alginate gel. Sepharose 77-84 decorin Homo sapiens 0-7 1313432-6 1992 The t-PA receptor binds to and can be eluted from wheat germ agglutinin-Sepharose. Sepharose 72-81 plasminogen activator, tissue type Rattus norvegicus 4-8 1545118-8 1992 IgD- Sepharose-purified IgD-BF was able to competitively inhibit rosetting of IgD-R+ T cells with IgD-coated RBC. Sepharose 5-14 immunoglobulin heavy constant delta Mus musculus 0-3 1545118-8 1992 IgD- Sepharose-purified IgD-BF was able to competitively inhibit rosetting of IgD-R+ T cells with IgD-coated RBC. Sepharose 5-14 immunoglobulin heavy constant delta Mus musculus 24-27 1545118-8 1992 IgD- Sepharose-purified IgD-BF was able to competitively inhibit rosetting of IgD-R+ T cells with IgD-coated RBC. Sepharose 5-14 immunoglobulin heavy constant delta Mus musculus 24-27 1545118-8 1992 IgD- Sepharose-purified IgD-BF was able to competitively inhibit rosetting of IgD-R+ T cells with IgD-coated RBC. Sepharose 5-14 immunoglobulin heavy constant delta Mus musculus 24-27 1545118-13 1992 Finally, LTB9 as well as IgD-Sepharose, immunoprecipitated a 70-kDa protein from the lysates of biosynthetically labeled H9-CEM1 cells. Sepharose 29-38 immunoglobulin heavy constant delta Mus musculus 25-28 1544938-3 1992 GPIIb-IIIa was bound to an affinity matrix of GRGDSPK-coupled Sepharose 4B and was then treated with chymotrypsin. Sepharose 62-71 integrin subunit alpha 2b Homo sapiens 0-5 1626920-0 1992 A method for the determination of sex hormone binding globulin using Concanavalin A-sepharose. Sepharose 84-93 sex hormone binding globulin Homo sapiens 34-62 1543734-3 1992 A bile salt-dependent lipase (BSDL) was purified from either defatted powder of cod pyloric caeca or aqueous pancreatic extracts by combined affinity chromatography on cholate-Sepharose and gel filtration on Sephacryl S-200 HR. Sepharose 176-185 carboxyl ester lipase Homo sapiens 2-28 1543734-3 1992 A bile salt-dependent lipase (BSDL) was purified from either defatted powder of cod pyloric caeca or aqueous pancreatic extracts by combined affinity chromatography on cholate-Sepharose and gel filtration on Sephacryl S-200 HR. Sepharose 176-185 carboxyl ester lipase Homo sapiens 30-34 1641822-4 1992 Analysis of plasma-vWf multimers by agarose gel electrophoresis consistently demonstrated a subtle decrease in the largest vWf multimers. Sepharose 36-43 von Willebrand factor Homo sapiens 19-22 1641822-4 1992 Analysis of plasma-vWf multimers by agarose gel electrophoresis consistently demonstrated a subtle decrease in the largest vWf multimers. Sepharose 36-43 von Willebrand factor Homo sapiens 123-126 1387538-2 1992 A single-step purification was performed on a calmodulin-agarose affinity column, following solubilization with Triton X-100. Sepharose 57-64 calmodulin 1 Rattus norvegicus 46-56 1536566-1 1992 Pig liver squalene epoxidase (SE) has been partially purified from solubilized microsomes by DEAE-Sephacel and Blue Sepharose 4B chromatography. Sepharose 116-125 squalene epoxidase Sus scrofa 10-28 1536566-1 1992 Pig liver squalene epoxidase (SE) has been partially purified from solubilized microsomes by DEAE-Sephacel and Blue Sepharose 4B chromatography. Sepharose 116-125 squalene epoxidase Sus scrofa 30-32 1533307-4 1992 The acylated and non-acylated plasminogen and plasmin were coupled to cyanogen bromide-activated Sepharose 4B and evaluated for streptokinase purification. Sepharose 97-109 plasminogen Homo sapiens 30-37 1616823-7 1992 The hPKC alpha overexpressing cells were able to grow in soft agarose after treatment with phorbol ester such as TPA (12-O-tetradecanoylphorbol 13-acetate). Sepharose 62-69 protein kinase C alpha Homo sapiens 4-14 1548051-6 1992 In addition, an immunoaffinity column of R1-20-5 coupled to Sepharose retained active renin but had a low affinity for prorenin. Sepharose 60-69 renin Homo sapiens 86-91 1582991-7 1992 The mitogenic activity of the unretained serum fraction was restored by the addition of PDGF AA, AB, or BB dimers or of a fraction (RF I) that dissociated from Heparin-Sepharose at 0.2 to 0.6 M NaCl. Sepharose 168-177 ring finger protein 34 Homo sapiens 132-136 1560180-1 1992 We have made a significant improvement in the electroelution device, Elutrap (Schleicher and Schuell) by substituting an agarose gel barrier, which is made from 0.6% agarose (SeaKem GTG; FMC Corporation), into the elution chamber in place of the manufacturer specified BT2 membrane. Sepharose 166-173 gamma-glutamyltransferase 1 Homo sapiens 182-185 1587806-4 1992 The affinity of rat enzyme to rat apo-A-I was stronger than that of human enzyme to human apo-A-I when estimated on the apo-A-I-Sepharose 4B column. Sepharose 128-137 apolipoprotein A1 Rattus norvegicus 34-41 1587806-4 1992 The affinity of rat enzyme to rat apo-A-I was stronger than that of human enzyme to human apo-A-I when estimated on the apo-A-I-Sepharose 4B column. Sepharose 128-137 apolipoprotein A1 Homo sapiens 90-97 1587806-4 1992 The affinity of rat enzyme to rat apo-A-I was stronger than that of human enzyme to human apo-A-I when estimated on the apo-A-I-Sepharose 4B column. Sepharose 128-137 apolipoprotein A1 Rattus norvegicus 90-97 1639209-4 1992 Preliminary characterisation has excluded EGF and TGF alpha, and demonstrated that the activity is bound reversibly by heparin-Sepharose, thus pointing to a member of the heparin-binding fibroblast- or hepatocyte-growth factor families. Sepharose 127-136 epidermal growth factor Homo sapiens 42-45 1639209-4 1992 Preliminary characterisation has excluded EGF and TGF alpha, and demonstrated that the activity is bound reversibly by heparin-Sepharose, thus pointing to a member of the heparin-binding fibroblast- or hepatocyte-growth factor families. Sepharose 127-136 transforming growth factor alpha Homo sapiens 50-59 1286675-1 1992 Interphotoreceptor retinoid-binding protein, IRBP, not only functioning as a shuttle to carry the retinoid between photoreceptor cells and pigment epithelium, but also inducing experimental autoimmune uveoretinitis (EAU), was purified by ConA Sepharose affinity chromatography from the fractions containing IRBP obtained in the course of ion-exchange chromatography by which the bovine retinal S-antigen was purified. Sepharose 243-252 retinol binding protein 3 Bos taurus 0-43 1286675-1 1992 Interphotoreceptor retinoid-binding protein, IRBP, not only functioning as a shuttle to carry the retinoid between photoreceptor cells and pigment epithelium, but also inducing experimental autoimmune uveoretinitis (EAU), was purified by ConA Sepharose affinity chromatography from the fractions containing IRBP obtained in the course of ion-exchange chromatography by which the bovine retinal S-antigen was purified. Sepharose 243-252 retinol binding protein 3 Bos taurus 45-49 1425044-3 1992 The authors first purified IRBP in China by Con A Sepharose affinity chromatography in conjunction with the purification of bovine retinal S-antigen by ion-exchange chromatography. Sepharose 50-59 retinol binding protein 3 Bos taurus 27-31 1441433-3 1992 The extraction of GP120 from culture fluid with immunosorbent based on sepharose 4B with ligated immunoglobulins from HIV-1-infected persons enriched the preparation for immunoblot with proteins increasing its diagnostic value. Sepharose 71-80 Envelope surface glycoprotein gp160, precursor Human immunodeficiency virus 1 18-23 1544444-2 1992 Interaction between calmodulin and human lactoferrin, a 78 kDa protein with antibacterial properties, was found in the presence of Ca2+ using (i) a method for the detection of calmodulin binding proteins with biotinylated calmodulin, (ii) affinity chromatography on an agarose-calmodulin column with subsequent detection by an enzyme-linked immunosorbent assay (ELISA). Sepharose 269-276 calmodulin 1 Homo sapiens 20-30 1544444-2 1992 Interaction between calmodulin and human lactoferrin, a 78 kDa protein with antibacterial properties, was found in the presence of Ca2+ using (i) a method for the detection of calmodulin binding proteins with biotinylated calmodulin, (ii) affinity chromatography on an agarose-calmodulin column with subsequent detection by an enzyme-linked immunosorbent assay (ELISA). Sepharose 269-276 calmodulin 1 Homo sapiens 176-186 1544444-2 1992 Interaction between calmodulin and human lactoferrin, a 78 kDa protein with antibacterial properties, was found in the presence of Ca2+ using (i) a method for the detection of calmodulin binding proteins with biotinylated calmodulin, (ii) affinity chromatography on an agarose-calmodulin column with subsequent detection by an enzyme-linked immunosorbent assay (ELISA). Sepharose 269-276 calmodulin 1 Homo sapiens 176-186 1544444-2 1992 Interaction between calmodulin and human lactoferrin, a 78 kDa protein with antibacterial properties, was found in the presence of Ca2+ using (i) a method for the detection of calmodulin binding proteins with biotinylated calmodulin, (ii) affinity chromatography on an agarose-calmodulin column with subsequent detection by an enzyme-linked immunosorbent assay (ELISA). Sepharose 269-276 calmodulin 1 Homo sapiens 176-186 1417990-3 1992 P26 was expressed in E. coli as a fusion protein and, after purification by affinity chromatography on IgG-Sepharose 6, cleaved off with enteropeptidase. Sepharose 107-116 recoverin Bos taurus 0-3 1311552-7 1992 Control and experimental pEX-1-Na+/H+ exchanger protein was purified on a p-aminophenyl beta-D-thiogalactopyranoside-agarose column. Sepharose 117-124 peroxisome biogenesis factor 1 Oryctolagus cuniculus 25-30 1531309-9 1992 Fc epsilon RI+ cells sorted at day 3 and cultured in agarose with IL-3 gave rise to 4,800 small and 150 medium-size mast cell colony-forming units per 10(6) cells, while Fc epsilon RI- cells gave rise to 23 medium-size and 49 large mast cell colony-forming units per 10(6) cells. Sepharose 53-60 Fc receptor, IgE, high affinity I, gamma polypeptide Mus musculus 0-13 1531309-9 1992 Fc epsilon RI+ cells sorted at day 3 and cultured in agarose with IL-3 gave rise to 4,800 small and 150 medium-size mast cell colony-forming units per 10(6) cells, while Fc epsilon RI- cells gave rise to 23 medium-size and 49 large mast cell colony-forming units per 10(6) cells. Sepharose 53-60 interleukin 3 Mus musculus 66-70 1531335-2 1992 GlcNAcT-V was purified by sequential affinity chromatography using first UDP-hexanolamine-agarose and then a synthetic oligosaccharide inhibitor-agarose column. Sepharose 90-97 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase Rattus norvegicus 0-9 1367986-5 1992 bFGF was purified close to homogeneity using a Heparin-Sepharose column and Mono S cation exchange chromatography. Sepharose 55-64 fibroblast growth factor 2 Homo sapiens 0-4 1349516-2 1992 Amniotic fluid cholinesterases (mixture of acetylcholinesterase and butyrylcholinesterase) purified by procainamide-Sepharose affinity chromatography exhibited aryl acylamidase activity which was sensitive to serotonin inhibition (a property of aryl acylamidases associated with both acetyl- and butyrylcholinesterases) and tyramine activation (shown exclusively by aryl acylamidase associated with butyrylcholinesterase). Sepharose 116-125 acetylcholinesterase (Cartwright blood group) Homo sapiens 43-63 1349516-2 1992 Amniotic fluid cholinesterases (mixture of acetylcholinesterase and butyrylcholinesterase) purified by procainamide-Sepharose affinity chromatography exhibited aryl acylamidase activity which was sensitive to serotonin inhibition (a property of aryl acylamidases associated with both acetyl- and butyrylcholinesterases) and tyramine activation (shown exclusively by aryl acylamidase associated with butyrylcholinesterase). Sepharose 116-125 butyrylcholinesterase Homo sapiens 68-89 1632506-2 1992 In this assay an agarose replica of the polyacrylamide gel which contains hyaluronic acid and bovine serum albumin (BSA) was used. Sepharose 17-24 albumin Homo sapiens 101-114 1540178-2 1992 VEGF was partially purified from the conditioned medium of transfected cells using heparin-sepharose affinity chromatography. Sepharose 91-100 vascular endothelial growth factor A Homo sapiens 0-4 1320300-5 1992 The inhibitor activity was due to the presence of polyclonal IgGs which bound to thrombin-Sepharose. Sepharose 90-99 coagulation factor II, thrombin Homo sapiens 81-89 1733970-1 1992 A beta-N-Acetylglucosaminide alpha 1----3-fucosyltransferase was purified from human serum by ammonium sulfate precipitation, hydrophobic chromatography on phenyl-Sepharose, ion-exchange chromatography on sulfopropyl-Sepharose, affinity chromatography on GDP-hexanolamine-Sepharose, and finally high pressure liquid chromatography gel filtration. Sepharose 163-172 fucosyltransferase 11 Homo sapiens 29-60 1733970-1 1992 A beta-N-Acetylglucosaminide alpha 1----3-fucosyltransferase was purified from human serum by ammonium sulfate precipitation, hydrophobic chromatography on phenyl-Sepharose, ion-exchange chromatography on sulfopropyl-Sepharose, affinity chromatography on GDP-hexanolamine-Sepharose, and finally high pressure liquid chromatography gel filtration. Sepharose 217-226 fucosyltransferase 11 Homo sapiens 29-60 1731608-1 1992 Human placental leucine aminopeptidase (P-LAP) was purified from retroplacental serum for the first time by serial chromatography on columns of Matrex Blue A, DEAE-Sepharose CL-6B, phenyl-Sepharose 4B, chelating-Sepharose, and Sepharose CL-6B. Sepharose 164-173 leucyl and cystinyl aminopeptidase Homo sapiens 40-45 1616632-2 1992 After the cryogel was solubilized at 37 degrees C, 1:1 complex of fibrinogen and fibronectin was purified at room temperature by affinity chromatography on a gelatin-Sepharose 4B. Sepharose 166-175 fibrinogen beta chain Homo sapiens 66-76 1616632-2 1992 After the cryogel was solubilized at 37 degrees C, 1:1 complex of fibrinogen and fibronectin was purified at room temperature by affinity chromatography on a gelatin-Sepharose 4B. Sepharose 166-175 fibronectin 1 Homo sapiens 81-92 1730869-11 1992 Affinity-purification of GIF in the DEAE Sepharose fraction by using anti-GIF-coupled Affigel, and analysis of the purified GIF by SDS-PAGE revealed that human GIF is a single polypeptide chain of 14 to 15 kDa. Sepharose 41-50 cobalamin binding intrinsic factor Homo sapiens 25-28 1732379-11 1992 The observed ability of dapsone to inhibit neutrophil chemotaxis under agarose to FMLP and interleukin-8 may also be explained by interference with integrin-mediated adherence required for motility in this assay system. Sepharose 71-78 formyl peptide receptor 1 Homo sapiens 82-86 1560789-3 1992 The region containing intervening sequence 25 of the COL3A1 gene was amplified using the polymerase chain reaction and the products were analyzed by agarose gel electrophoresis and dideoxynucleotide sequencing. Sepharose 149-156 collagen type III alpha 1 chain Homo sapiens 53-59 1330132-2 1992 When detergent extracts of bovine cardiac mitochondria were applied to either a poly-L-lysine-agarose or a lysine-Sepharose column at low ionic strength, cytochrome c oxidase was found to adhere tightly, whereas the bulk of the proteins were eluted by washing with the same buffer. Sepharose 114-123 cytochrome c oxidase subunit 6A1, mitochondrial Bos taurus 154-174 1741416-3 1992 We have employed IgG and IgE-coated Sepharose beads to investigate selective modulation of IgG and IgE-mediated enzyme release by IL-1 beta. Sepharose 36-45 interleukin 1 beta Homo sapiens 130-139 1370959-7 1992 MAb 1D11 coupled to Sepharose was suitable for immunoextraction of PTHRP, and successfully localised PTHRP on immunoblots. Sepharose 20-29 parathyroid hormone like hormone Homo sapiens 67-72 1309805-2 1992 We show here that the Eg1 gene product (cdk2) possesses histone H1 protein kinase activity and binds to PSTAIR antibodies as well as to Sepharose beads linked to the 13-kDa product of the suc 1 gene (p13suc1). Sepharose 136-145 cyclin-dependent kinase 2 S homeolog Xenopus laevis 22-25 1309805-2 1992 We show here that the Eg1 gene product (cdk2) possesses histone H1 protein kinase activity and binds to PSTAIR antibodies as well as to Sepharose beads linked to the 13-kDa product of the suc 1 gene (p13suc1). Sepharose 136-145 cyclin-dependent kinase 2 S homeolog Xenopus laevis 40-44 1730728-6 1992 These two apoE variants were purified from cell lysates of the transfected Escherichia coli by ultracentrifugal flotation in the presence of phospholipid, by gel filtration chromatography, and by heparin-Sepharose chromatography. Sepharose 204-213 apolipoprotein E Homo sapiens 10-14 1377416-10 1992 Many antibodies quench also binding of t-PA to lysine-Sepharose. Sepharose 54-63 plasminogen activator, tissue type Homo sapiens 39-43 1615470-5 1992 By SDS-agarose gel electrophoresis, the percentage of high molecular weight forms of vWF varied from 39 to 49% relative to normal plasma for BHK, CHO, 143B and chicken cells but was less than 10% for L cells. Sepharose 7-14 von Willebrand factor Mesocricetus auratus 85-88 1363453-1 1992 Arylsulfatase A (arylsulfatase sulfohydrolase) EC 3.1.6.1 was purified from rat liver by a procedure consisting of differential centrifugation, Con A-Sepharose and Blue Sepharose chromatography, PBE 94 chromatofocusing, DEAE-cellulose and gel filtration chromatography followed by preparative electrophoresis. Sepharose 150-159 arylsulfatase A Rattus norvegicus 0-15 1283495-2 1992 Concentration of bFGF in cell extract was measured by sandwich radioimmunoassay (RIA) with heparin-Sepharose and 125I-labeled monoclonal antibody. Sepharose 99-108 fibroblast growth factor 2 Rattus norvegicus 17-21 1736938-5 1992 ConA did not appear to bind to the V1 domain of CD4 at the U937 cell surface since Leu3a binding was not blocked in the presence of ConA, nor was recombinant CD4 retained on a ConA-agarose affinity matrix. Sepharose 181-188 CD4 molecule Homo sapiens 158-161 1595899-1 1992 Triton-solubilized rabbit mammary prolactin (PRL) receptor was purified by concanavalin A-agarose chromatography and immobilized on a nitrocellulose membrane. Sepharose 90-97 prolactin receptor Oryctolagus cuniculus 34-58 1515867-1 1992 Calmodulin was purified from human tonsillar lymphocytes utilizing calcium-dependent binding of calmodulin to fluphenazine-Sepharose. Sepharose 123-132 calmodulin 1 Homo sapiens 0-10 1515867-1 1992 Calmodulin was purified from human tonsillar lymphocytes utilizing calcium-dependent binding of calmodulin to fluphenazine-Sepharose. Sepharose 123-132 calmodulin 1 Homo sapiens 96-106 1642965-1 1992 The effect of phenprocoumon enantiomers on the stereoselective binding of 3-substituted 1,4-benzodiazepines to human serum albumin (HSA) was studied by chromatography on HSA-Sepharose column. Sepharose 174-183 albumin Homo sapiens 117-130 1468208-9 1992 TIMP-1 (28 kDa) and TIMP-2 (21 kDa) were also identified by immunoblotting of gelatin Sepharose bound plasma proteins using non-crossreacting antibodies to each protein. Sepharose 86-95 TIMP metallopeptidase inhibitor 1 Homo sapiens 0-6 1306686-2 1992 AR possesses two putative nuclear localization sequences (NLS), binds to DNA sepharose, and localizes to the nucleoli of human ovarian surface epithelial carcinoma cells suggesting that AR has a direct nuclear role. Sepharose 77-86 amphiregulin Homo sapiens 0-2 1643445-3 1992 Profilin was purified from Lolium perenne grass pollen by means of affinity purification with Sepharose-coupled poly(L-proline). Sepharose 94-103 profilin Solanum tuberosum 0-8 1373999-6 1992 Antibodies to MBP were isolated from purified CSF IgG of MS patients with acute relapses by two-step antigen specific (MBP-Sepharose) affinity chromatography. Sepharose 123-132 myelin basic protein Homo sapiens 14-17 1363453-1 1992 Arylsulfatase A (arylsulfatase sulfohydrolase) EC 3.1.6.1 was purified from rat liver by a procedure consisting of differential centrifugation, Con A-Sepharose and Blue Sepharose chromatography, PBE 94 chromatofocusing, DEAE-cellulose and gel filtration chromatography followed by preparative electrophoresis. Sepharose 169-178 arylsulfatase A Rattus norvegicus 0-15 1373999-6 1992 Antibodies to MBP were isolated from purified CSF IgG of MS patients with acute relapses by two-step antigen specific (MBP-Sepharose) affinity chromatography. Sepharose 123-132 myelin basic protein Homo sapiens 119-122 1542088-9 1992 Using polymerase chain reaction (PCR) amplification and agarose gel electrophoresis, deletions in the HPRT promoter have been observed and correlated to deficient enzyme expression. Sepharose 56-63 hypoxanthine guanine phosphoribosyl transferase Mus musculus 102-106 1552475-3 1992 The removal of hCG from the purified preparation with anti-hCG Sepharose 4B abolished the vasopermeability effect of the preparation, confirming that hCG itself is modified in such a way as to produce the response. Sepharose 63-75 chorionic gonadotropin subunit beta 5 Homo sapiens 15-18 1552475-3 1992 The removal of hCG from the purified preparation with anti-hCG Sepharose 4B abolished the vasopermeability effect of the preparation, confirming that hCG itself is modified in such a way as to produce the response. Sepharose 63-75 chorionic gonadotropin subunit beta 5 Homo sapiens 59-62 1552475-3 1992 The removal of hCG from the purified preparation with anti-hCG Sepharose 4B abolished the vasopermeability effect of the preparation, confirming that hCG itself is modified in such a way as to produce the response. Sepharose 63-75 chorionic gonadotropin subunit beta 5 Homo sapiens 59-62 1359387-1 1992 We have provided a detailed protocol for the synthesis of a yohimbine-agarose matrix that has been shown to be effective for isolation of the alpha 2A-adrenergic receptor from human platelet and purification of the alpha 2A-adrenergic receptor to apparent homogeneity from porcine brain cortex using chromatography on only two sequential yohimbine-agarose columns. Sepharose 70-77 adrenoceptor alpha 2A Homo sapiens 142-170 1740645-4 1992 Plasma Fn was purified from the blood by gelatin-Sepharose affinity chromatography. Sepharose 49-58 fibronectin 1 Homo sapiens 7-9 1359387-1 1992 We have provided a detailed protocol for the synthesis of a yohimbine-agarose matrix that has been shown to be effective for isolation of the alpha 2A-adrenergic receptor from human platelet and purification of the alpha 2A-adrenergic receptor to apparent homogeneity from porcine brain cortex using chromatography on only two sequential yohimbine-agarose columns. Sepharose 70-77 adrenoceptor alpha 2A Homo sapiens 215-243 1359387-1 1992 We have provided a detailed protocol for the synthesis of a yohimbine-agarose matrix that has been shown to be effective for isolation of the alpha 2A-adrenergic receptor from human platelet and purification of the alpha 2A-adrenergic receptor to apparent homogeneity from porcine brain cortex using chromatography on only two sequential yohimbine-agarose columns. Sepharose 348-355 adrenoceptor alpha 2A Homo sapiens 142-170 1764068-1 1991 S-Adenosylmethionine decarboxylase (EC 4.1.1.19) was purified to homogeneity from the cytosol of soybean (Glycine max) axes by ammonium sulfate fractionation, DEAE-Sepharose and methylglyoxalbis(guanylhydrazone)-Sepharose 6B chromatographies. Sepharose 164-173 S-adenosylmethionine decarboxylase Glycine max 0-34 1378467-6 1992 Studies on mucin polymer assembly revealed that mucin preparations prepared by equilibrium density gradient centrifugation and Sepharose 2B chromatography are not completely purified and contain DNA and extraneous proteins. Sepharose 127-136 LOC100508689 Homo sapiens 48-53 1777984-3 1991 Two distinct zones of PLA2 activity were found on agarose electrophoresis of purified human PLA2 in the presence of albumin, and in the sera from the acute pancreatitis patients. Sepharose 50-57 phospholipase A2 group IB Homo sapiens 22-26 1761551-4 1991 Polyclonal antibodies raised against this peptide and immunoaffinity-purified on a protein C-Sepharose column inhibited APC anticoagulant activity in activated partial thromboplastin time and Xa-1-stage assays in normal, protein S-depleted, and Factor VIII-deficient plasma with half-maximal inhibition at 30 nM anti-(390-404) antibody. Sepharose 93-102 APC regulator of WNT signaling pathway Homo sapiens 120-123 1742731-6 1991 U-77 was also found to induce DNA interstrand cross-links in naked DNA, as measured by an agarose gel method. Sepharose 90-97 small nucleolar RNA, C/D box 77 Homo sapiens 0-4 1744125-2 1991 Betaglycan solubilized from rat embryo membrane preparations was purified to near-homogeneity by sequential chromatography through DEAE-Trisacryl, wheat germ lectin-Sepharose, and TGF-beta 1-agarose. Sepharose 165-174 transforming growth factor beta receptor 3 Rattus norvegicus 0-10 1661685-5 1991 This novel band could be identified as ubiquityl-calmodulin by the following methods: (i) identical Rf-value of novel conjugate and standard uCaM in SDS-PAGE; (ii) Ca(2+)-dependent conjugate formation; (iii) Ca(2+)-dependent adsorption to fluphenazine-Sepharose; (iv) Ca(2+)-dependent mobility change of the novel conjugate during SDS-PAGE; and (v) inhibition of conjugate band formation by phosphorylase kinase. Sepharose 252-261 calmodulin Oryctolagus cuniculus 49-59 1809336-0 1991 Cloning, restriction digestion and DNA labeling of large DNA fragments (greater than or equal to 1 kb) in the presence of remelted SeaPlaque GTG agarose gels. Sepharose 145-152 gamma-glutamyltransferase 1 Homo sapiens 141-144 1959989-1 1991 A novel 21-kDa protein (p21) was isolated from auto-immune complexes, found in sera of 14 patients with malignant urogenital tumors and isolated on Protein A-Sepharose. Sepharose 158-167 H3 histone pseudogene 16 Homo sapiens 24-27 1804104-2 1991 We here report on the preparation of an immunoaffinity chromatographic system (high affinity digoxin-binding antibodies (Fab fragments) bound covalently to Sepharose) for the purification of endogenous digitalis like factor(s). Sepharose 156-165 FA complementation group B Homo sapiens 121-124 1804104-4 1991 Endogenous digitalis like factor(s) absorbed to Sepharose coupled to Fab fragments can be eluted by methanol. Sepharose 48-57 FA complementation group B Homo sapiens 69-72 1777419-4 1991 Cells were incubated with biotin-IL-3 at 4 degrees C and IL-3 bound to the low affinity site was removed by washing, cells were detergent extracted, and then streptavidin - agarose was used to purify proteins bound to biotin-IL-3. Sepharose 173-180 interleukin 3 Mus musculus 57-61 1777419-4 1991 Cells were incubated with biotin-IL-3 at 4 degrees C and IL-3 bound to the low affinity site was removed by washing, cells were detergent extracted, and then streptavidin - agarose was used to purify proteins bound to biotin-IL-3. Sepharose 173-180 interleukin 3 Mus musculus 57-61 1658224-5 1991 To examine the role of protein tyrosine phosphorylation in the activation of the MAP-2 kinase, we isolated phosphotyrosine (PTyr)-containing proteins from chromaffin cells by immunoaffinity adsorption on anti-PTyr-Sepharose beads. Sepharose 214-223 microtubule associated protein 2 Bos taurus 81-86 1658224-6 1991 Anti-PTyr-Sepharose eluates from IGF-I-treated cells showed increased MAP-2 kinase activity; thus, the MAP-2 kinase (or a closely associated protein) appears to be a PTyr-containing protein. Sepharose 10-19 insulin like growth factor 1 Bos taurus 33-38 1658224-6 1991 Anti-PTyr-Sepharose eluates from IGF-I-treated cells showed increased MAP-2 kinase activity; thus, the MAP-2 kinase (or a closely associated protein) appears to be a PTyr-containing protein. Sepharose 10-19 microtubule associated protein 2 Bos taurus 70-75 1658224-6 1991 Anti-PTyr-Sepharose eluates from IGF-I-treated cells showed increased MAP-2 kinase activity; thus, the MAP-2 kinase (or a closely associated protein) appears to be a PTyr-containing protein. Sepharose 10-19 microtubule associated protein 2 Bos taurus 103-108 1685016-4 1991 Secreted NEU protein possessing only the extracellular domain but lacking transmembrane and protein kinase domains was expressed in HeLa cells and then purified from conditioned medium, using affinity chromatography on WGA-Sepharose. Sepharose 223-232 erb-b2 receptor tyrosine kinase 2 Homo sapiens 9-12 1660262-5 1991 The results show that the removal of 34 amino acids from the C-terminal end of InsP3 3-kinase resulted in an inactive protein which still interacted with CaM-Sepharose in a Ca2(+)-dependent way. Sepharose 158-167 calmodulin 1 Rattus norvegicus 154-157 1660262-9 1991 Affinity chromatography on CaM-Sepharose of 5" and 3" deletion mutants revealed that the sequence stretching from Ser-156 to Leu-189 is involved in CaM binding and enzyme stimulation. Sepharose 31-40 calmodulin 1 Rattus norvegicus 27-30 1660262-9 1991 Affinity chromatography on CaM-Sepharose of 5" and 3" deletion mutants revealed that the sequence stretching from Ser-156 to Leu-189 is involved in CaM binding and enzyme stimulation. Sepharose 31-40 calmodulin 1 Rattus norvegicus 148-151 1835839-1 1991 Various amounts of the activation fragment C3b of the complement (C) protein C3 were coupled to Sepharose 4B by catalysis with the C3 convertase of the alternative pathway of C. The binding of radioactively labelled C proteins B and H (= factor H) to the C3b-carrying particles was assayed. Sepharose 96-105 complement C3 Homo sapiens 43-46 1929427-4 1991 There was preferential binding of the [35S]TfR to Con A-Sepharose, indicating the existence of a higher density of high mannose chains on the 35S-labeled TfR. Sepharose 56-65 transferrin receptor protein 1 Ovis aries 43-46 1657983-10 1991 Biosynthetically labeled 40-kDa protein coprecipitated with t-PA- or Lys-PLG-Sepharose beads, but not with unconjugated Sepharose. Sepharose 77-86 plasminogen Homo sapiens 73-76 1719984-3 1991 Phosphorylated EGF receptor bound to immobilized SH2/SH3 of PLC gamma 1 in Sepharose beads, while nonphosphorylated EGF receptor did not bind. Sepharose 75-84 phospholipase C gamma 1 Homo sapiens 60-71 1834652-5 1991 Sepharose-immobilized ceramide and Sepharose-immobilized glucosylceramide were found to be suitable acceptors for GlcT and GalT-2, respectively, still using intact Golgi cisternae as the enzyme source. Sepharose 0-9 beta-1,3-galactosyltransferase 4 Homo sapiens 123-129 1834652-5 1991 Sepharose-immobilized ceramide and Sepharose-immobilized glucosylceramide were found to be suitable acceptors for GlcT and GalT-2, respectively, still using intact Golgi cisternae as the enzyme source. Sepharose 35-44 beta-1,3-galactosyltransferase 4 Homo sapiens 123-129 1656877-2 1991 Incubation of cytosolic [3H]TCDD-aryl hydrocarbon (Ah) receptor from wild-type Hepa 1c1c7 cells for 16 h at 4 degrees C in 0.4 M KCl resulted in the formation of transformed liganded receptor which exhibited increased binding affinity on DNA-Sepharose columns. Sepharose 242-251 aryl-hydrocarbon receptor Mus musculus 24-63 1937785-2 1991 The inhibitory activity could be detected after the depletion of IL-1 alpha by the use of a specific antibody (anti-human recombinant IL-1 alpha monoclonal antibody)-conjugated Sepharose column. Sepharose 177-186 interleukin 1 alpha Homo sapiens 65-75 1937785-2 1991 The inhibitory activity could be detected after the depletion of IL-1 alpha by the use of a specific antibody (anti-human recombinant IL-1 alpha monoclonal antibody)-conjugated Sepharose column. Sepharose 177-186 interleukin 1 alpha Homo sapiens 134-144 1959923-2 1991 The amplified fragment was examined by direct agarose gel electrophoresis, and a deletion of 20 base pairs (bp) in exon 6 of the LDH-A gene was found. Sepharose 46-53 lactate dehydrogenase A Homo sapiens 129-134 1717490-4 1991 HNK-1-positive PI-GP150 was purified from the PI-PLC-released materials with three successive chromatographies (Sephacryl S-300, mAb HNK-1-Sepharose 4B, and Mono Q) and proven to be a novel molecule by immunoblot and structural analyses. Sepharose 139-148 beta-1,3-glucuronyltransferase 1 Rattus norvegicus 0-5 1762032-6 1991 DNA extracted from apoptotic cells cultured with TNF alpha and IFN gamma was fragmented, and a set of bands of the "200 bp ladder", which is characteristic of the DNA of apoptotic cells, was observed in agarose gel electrophoresis. Sepharose 203-210 tumor necrosis factor Rattus norvegicus 49-58 1762032-6 1991 DNA extracted from apoptotic cells cultured with TNF alpha and IFN gamma was fragmented, and a set of bands of the "200 bp ladder", which is characteristic of the DNA of apoptotic cells, was observed in agarose gel electrophoresis. Sepharose 203-210 interferon gamma Rattus norvegicus 63-72 1795446-8 1991 The author recently reported that the apo C of high-density lipoprotein (HDL-apo C) was detected in alpha lipoprotein, but that HDL-apo E was detected in the near alpha 2-globulin region behind alpha lipoprotein on agarose-gel immunofixation electrophoresis. Sepharose 215-222 apolipoprotein E Homo sapiens 132-137 1681618-4 1991 A single major virus-specific polypeptide of Mr = 25,000 (p25) bound to the poly(rI).poly(rC)-Sepharose. Sepharose 94-103 tubulin polymerization promoting protein Homo sapiens 58-61 1718413-1 1991 Rabbit serum paraoxonase/arylesterase has been purified to homogeneity by Cibacron Blue-agarose chromatography, gel filtration, DEAE-Trisacryl M chromatography, and preparative SDS gel electrophoresis. Sepharose 88-95 serum paraoxonase/arylesterase 1 Oryctolagus cuniculus 7-24 1722405-3 1991 Similarly, insulin-stimulated autophosphorylation and kinase activity of the insulin receptor purified on wheat germ agglutinin-agarose from pertussis toxin-treated FaO cells was diminished 50%; however, treatment of cells with the catalytically inactive B-oligomer of the toxin had no effect on receptor tyrosine kinase activity in vitro. Sepharose 128-135 insulin receptor Rattus norvegicus 77-93 1955119-2 1991 The response of lamina propria T lymphocytes to Sepharose-bound anti-CD3 antibody plus interleukin 2 was significantly lower than the response of autologous peripheral blood T lymphocytes, whereas the responses of lamina propria T lymphocytes to anti-T11(2/3) antibodies plus sheep erythrocytes or anti-CD28 antibody plus interleukin 2 were largely preserved. Sepharose 48-57 T-cell-specific surface glycoprotein CD28 Ovis aries 303-307 1955119-2 1991 The response of lamina propria T lymphocytes to Sepharose-bound anti-CD3 antibody plus interleukin 2 was significantly lower than the response of autologous peripheral blood T lymphocytes, whereas the responses of lamina propria T lymphocytes to anti-T11(2/3) antibodies plus sheep erythrocytes or anti-CD28 antibody plus interleukin 2 were largely preserved. Sepharose 48-57 interleukin-2 Ovis aries 322-335 1791429-1 1991 Each of two affinity isolation methods, the first based on biotinylated porcine transferrin plus streptavidin-agarose, and the second on Sepharose-coupled porcine transferrin, followed by SDS-PAGE, allowed the isolation and identification of two potential porcine-transferrin-binding polypeptides (approximately 64 kDa and 99 kDa) from total membranes of Actinobacillus pleuropneumoniae grown under iron-restricted conditions. Sepharose 137-146 transferrin Homo sapiens 163-174 1791429-1 1991 Each of two affinity isolation methods, the first based on biotinylated porcine transferrin plus streptavidin-agarose, and the second on Sepharose-coupled porcine transferrin, followed by SDS-PAGE, allowed the isolation and identification of two potential porcine-transferrin-binding polypeptides (approximately 64 kDa and 99 kDa) from total membranes of Actinobacillus pleuropneumoniae grown under iron-restricted conditions. Sepharose 137-146 transferrin Homo sapiens 163-174 1791429-3 1991 The 64 kDa polypeptide was the more easily removed from Sepharose-coupled porcine transferrin and only the 99 kDa polypeptide appeared to be an outer-membrane protein. Sepharose 56-65 transferrin Homo sapiens 82-93 1811793-4 1991 Growth hormone was purified from human pituitaries and the differently charged forms were separated by column electrophoresis in agarose suspension. Sepharose 129-136 growth hormone 1 Homo sapiens 0-14 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 87-96 Fc gamma receptor Ia Homo sapiens 34-45 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 87-96 C-reactive protein Homo sapiens 212-215 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 87-96 Fc gamma receptor Ia Homo sapiens 234-245 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 105-114 Fc gamma receptor Ia Homo sapiens 34-45 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 105-114 C-reactive protein Homo sapiens 101-104 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 105-114 Fc gamma receptor Ia Homo sapiens 34-45 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 105-114 C-reactive protein Homo sapiens 101-104 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 105-114 Fc gamma receptor Ia Homo sapiens 34-45 1834740-13 1991 The identity of this protein with Fc gamma RI was confirmed by the ability of both IgG-Sepharose and CRP-Sepharose to preclear the protein from cell lysates and by inhibition of binding to both IgG-Sepharose and CRP-Sepharose by anti-Fc gamma RI mAb 197. Sepharose 105-114 C-reactive protein Homo sapiens 101-104 1953677-9 1991 On lipoprotein lipase-agarose the endothelial heparan sulphate chains were eluted at the same salt concentration as heparin, and the binding persisted, although with decreased strength, after digestion with heparinase. Sepharose 22-29 lipoprotein lipase Homo sapiens 3-21 1814436-5 1991 The resulting construct is able to drive the expression of a high amount of FGF-6 protein in Escherichia coli, which can be solubilized and purified through heparin-Sepharose chromatography and high salt elution. Sepharose 165-174 fibroblast growth factor 6 Mus musculus 76-81 1839507-6 1991 The IL-1 binding factor could be eliminated from plasma by incubation with protein A-Sepharose, suggesting that it consisted in IgG antibodies directed against IL-1. Sepharose 85-94 interleukin 1 beta Homo sapiens 4-8 1839507-6 1991 The IL-1 binding factor could be eliminated from plasma by incubation with protein A-Sepharose, suggesting that it consisted in IgG antibodies directed against IL-1. Sepharose 85-94 interleukin 1 beta Homo sapiens 160-164 1961198-1 1991 Interleukin 2 was bound to Sepharose beads in order to assess its potential bioactivity in vitro. Sepharose 27-36 interleukin 2 Homo sapiens 0-13 1810021-8 1991 Agarose electrophoresis of plasma with [125I]-ET-1 indicated a reversible binding to albumin. Sepharose 0-7 endothelin 1 Homo sapiens 46-50 1932084-7 1991 A single step method for the immunoaffinity purification of pig liver S-COMT was developed by using a Sepharose 4B column to which the mAb Co54-5F/8 was covalently coupled. Sepharose 102-111 catechol-O-methyltransferase Sus scrofa 72-76 1953650-11 1991 These data suggest that bovine thymus p56lck is responsible for the activity found in the early-eluting peak from heparin-agarose. Sepharose 122-129 lymphocyte protein tyrosine kinase Mus musculus 38-44 1917990-1 1991 A novel Ca(2+)-binding protein, tentatively designated calgizzarin, has been purified to apparent homogeneity from chicken gizzard smooth muscle by W-7 (N-(6-aminohexyl-5-chloro-1-naphthalenesulfonamide))-Sepharose affinity chromatography and ion-exchange chromatography. Sepharose 205-214 S100 calcium binding protein A11 Gallus gallus 55-66 1918001-2 1991 By using insulin that has been protected in positions A1 and B29, we have been able to couple the insulin selectively through the B1 amino group to divinyl sulfone-activated agarose. Sepharose 174-181 insulin Homo sapiens 9-16 1918001-2 1991 By using insulin that has been protected in positions A1 and B29, we have been able to couple the insulin selectively through the B1 amino group to divinyl sulfone-activated agarose. Sepharose 174-181 CD79b molecule Homo sapiens 61-64 1918001-2 1991 By using insulin that has been protected in positions A1 and B29, we have been able to couple the insulin selectively through the B1 amino group to divinyl sulfone-activated agarose. Sepharose 174-181 insulin Homo sapiens 98-105 1928066-7 1991 IgA-fibronectin aggregates also were detected in serum using an antifibronectin antibody capture assay; and could be depleted from serum by heparin-agarose affinity chromatography. Sepharose 148-155 fibronectin 1 Homo sapiens 4-15 1724753-1 1991 Hyaluronic acid binding protein (HABP) has been purified to homogeneity from normal adult rat kidney by hyaluronate Sepharose affinity chromatography, and its apparent molecular mass was found to be 68 kDa. Sepharose 116-125 hyaluronan binding protein 2 Rattus norvegicus 0-31 1724753-1 1991 Hyaluronic acid binding protein (HABP) has been purified to homogeneity from normal adult rat kidney by hyaluronate Sepharose affinity chromatography, and its apparent molecular mass was found to be 68 kDa. Sepharose 116-125 hyaluronan binding protein 2 Rattus norvegicus 33-37 1936188-13 1991 The RCA agarose eluted protein co-electrophoresed with a rhodopsin standard and was light sensitive. Sepharose 8-15 rhodopsin Rattus norvegicus 57-66 1660513-4 1991 The eluted protein from a calmodulin-coupled Sepharose 4B column with EGTA was analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis which revealed a major protein band of Mr 50,000. Sepharose 45-54 calmodulin 1 Rattus norvegicus 26-36 1961021-2 1991 TNF increased the appearance of very high molecular weight DNA fragments detected by agarose gel electrophoresis. Sepharose 85-92 tumor necrosis factor Homo sapiens 0-3 1798438-1 1991 An endopeptidase generating a peptide comprised of the first five amino acids of luteinizing hormone-releasing hormone (LHRH) was isolated form the membranes of glioma C6 cells by a procedure including Brij extraction, p-mercuribenzoate-Sepharose chromatography, and Mono Q high-performance liquid chromatography. Sepharose 237-246 gonadotropin releasing hormone 1 Homo sapiens 81-118 1798438-1 1991 An endopeptidase generating a peptide comprised of the first five amino acids of luteinizing hormone-releasing hormone (LHRH) was isolated form the membranes of glioma C6 cells by a procedure including Brij extraction, p-mercuribenzoate-Sepharose chromatography, and Mono Q high-performance liquid chromatography. Sepharose 237-246 gonadotropin releasing hormone 1 Homo sapiens 120-124 1821803-1 1991 Pneumocystis carinii dihydrofolate reductase (DHFR) expressed in Escherichia coli was purified to homogeneity in a single step using methotrexate-Sepharose affinity chromatography. Sepharose 146-155 Dihydrofolate reductase Escherichia coli 21-44 1821803-1 1991 Pneumocystis carinii dihydrofolate reductase (DHFR) expressed in Escherichia coli was purified to homogeneity in a single step using methotrexate-Sepharose affinity chromatography. Sepharose 146-155 Dihydrofolate reductase Escherichia coli 46-50 1767248-3 1991 Pure haptoglobin was obtained by the subsequent affinity chromatography on a rabbit-haemoglobin Sepharose column. Sepharose 96-105 haptoglobin Oryctolagus cuniculus 5-16 24213342-6 1991 These two proteins were further confirmed as HSP-70 cognates by their ability to bind to an ATP-agarose column. Sepharose 96-103 heat shock 70 kDa protein 4 Triticum aestivum 45-51 1655737-9 1991 An active recombinant catalytic subunit was expressed in bacteria and purified by CaM-Sepharose chromatography. Sepharose 86-95 calmodulin Bos taurus 82-85 1809336-1 1991 Large DNA fragments (greater than or equal to 1 kb), separated in low melting temperature SeaPlaque GTG agarose gels, can be enzymatically processed directly in the presence of this agarose (in-gel). Sepharose 104-111 gamma-glutamyltransferase 1 Homo sapiens 100-103 1655745-1 1991 A vasoactive intestinal peptide (VIP) binding protein was purified in active form by detergent solubilization of lung membranes, gel filtration, VIP-Sepharose affinity chromatography, reverse phase high performance liquid chromatography, and anion exchange chromatography. Sepharose 149-158 vasoactive intestinal peptide Rattus norvegicus 33-36 1655745-1 1991 A vasoactive intestinal peptide (VIP) binding protein was purified in active form by detergent solubilization of lung membranes, gel filtration, VIP-Sepharose affinity chromatography, reverse phase high performance liquid chromatography, and anion exchange chromatography. Sepharose 149-158 vasoactive intestinal peptide Rattus norvegicus 145-148 1809336-1 1991 Large DNA fragments (greater than or equal to 1 kb), separated in low melting temperature SeaPlaque GTG agarose gels, can be enzymatically processed directly in the presence of this agarose (in-gel). Sepharose 182-189 gamma-glutamyltransferase 1 Homo sapiens 100-103 1809336-2 1991 Time saving protocols are discussed for in-gel processing of large DNA fragments in the presence of remelted SeaPlaque GTG agarose, including cloning into pUC18, nick translation, random priming and restriction digestion. Sepharose 123-130 gamma-glutamyltransferase 1 Homo sapiens 119-122 1909888-7 1991 Amino acid sequence analysis of the Blue Sepharose eluate identified three protein chains--those of apolipoprotein A1 and immunoglobulin heavy and light chains--suggesting that the preparation is an apolipoprotein A1-immunoglobulin complex. Sepharose 41-50 apolipoprotein A1 Homo sapiens 199-216 1925827-1 1991 Human erythrocyte porphobilinogen deaminase was isolated using ammonium sulphate fractionation and heat treatment, Sephadex G-25 and G-100 chromatography, di-ethylamino-ethyl anion-exchange chromatography, chromatofocusing over a pH gradient of 7-4 and, finally, hydrophobic interaction chromatography on a phenyl-Sepharose column. Sepharose 314-323 hydroxymethylbilane synthase Homo sapiens 18-43 1797823-2 1991 H-FABP was purified by gel permeation chromatography on a Sephacryl S-200 column followed by anion-exchange chromatography on a Sepharose Q fast-flow column at pH 7.0. Sepharose 128-137 fatty acid binding protein 3 Homo sapiens 0-6 1909733-5 1991 We found that 22% of the IgA and 15% of the IgG F(ab")2 bound to SPA-agarose. Sepharose 69-76 surfactant protein A1 Homo sapiens 65-68 1654330-3 1991 To identify this LPL binding protein(s), radioiodinated cell surface proteins from cultured bovine aortic endothelial cells were chromatographed using bovine LPL-Sepharose. Sepharose 162-171 lipoprotein lipase Bos taurus 17-20 1654330-3 1991 To identify this LPL binding protein(s), radioiodinated cell surface proteins from cultured bovine aortic endothelial cells were chromatographed using bovine LPL-Sepharose. Sepharose 162-171 lipoprotein lipase Bos taurus 158-161 1654330-7 1991 A 220-kDa protein from the basal cell surface was also identified using LPL-Sepharose chromatography. Sepharose 76-85 lipoprotein lipase Bos taurus 72-75 1894620-1 1991 Rat mast cell proteinase II (RMCP II) from mucosal mast cells was titrated into rat serum, and the resulting serine proteinase inhibitor (serpin)-enzyme complex was purified by affinity chromatography on anti-RMCP II-Sepharose 4B and by Mono-Q anion-exchange. Sepharose 217-226 mast cell protease 2 Rattus norvegicus 209-216 1894620-2 1991 The purified complex was used to raise polyclonal antibodies which, after cross-absorption against RMCP II-Sepharose 4B, were specific for serpin and were used to affinity purify two rat serpin molecules (RSI and RSII) that inhibit RMCP II in rat serum. Sepharose 107-116 mast cell protease 2 Rattus norvegicus 99-106 1892900-6 1991 Furthermore, immunoprecipitation and protein A-Sepharose adsorption analysis revealed that in the calf uterine cytosol, the [3H]R5020-receptor complexes were recognized by anti-progesterone receptor monoclonal antibody PR6. Sepharose 47-56 progesterone receptor Bos taurus 177-198 1910811-9 1991 The chemotaxin was shown to be fibronectin, since activity was abolished, in a dose-dependent manner, by treatment with anti-rat fibronectin antiserum as well as by passage through a gelatin agarose affinity column. Sepharose 191-198 fibronectin 1 Rattus norvegicus 31-42 1686394-1 1991 In rat hepatocytes; the tumorigenic rat liver cell line ARL-16; and the human hepatoma line, Hep G2, 50% of the total gamma-glutamyl transpeptidase (GGT) activity was bound by a Concanavalin-A Sepharose 4B column, calling for alpha-methylmannoside elution (Peak I). Sepharose 193-202 inactive glutathione hydrolase 2 Homo sapiens 118-147 1911389-3 1991 Accordingly, the antithrombin was isolated by heparin-Sepharose chromatography: this produced a mixture of normal and variant antithrombin, as the patient was heterozygous for the abnormality. Sepharose 54-63 serpin family C member 1 Homo sapiens 17-29 1911389-4 1991 To remove the normal component, the antithrombin was passed through a column of thrombin-Sepharose. Sepharose 89-98 serpin family C member 1 Homo sapiens 36-48 1911389-4 1991 To remove the normal component, the antithrombin was passed through a column of thrombin-Sepharose. Sepharose 89-98 coagulation factor II, thrombin Homo sapiens 40-48 1911389-5 1991 On sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), prior to its application to thrombin-Sepharose, the antithrombin migrated as a single band with identical mobility to that of normal antithrombin. Sepharose 111-120 coagulation factor II, thrombin Homo sapiens 102-110 1911389-5 1991 On sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), prior to its application to thrombin-Sepharose, the antithrombin migrated as a single band with identical mobility to that of normal antithrombin. Sepharose 111-120 serpin family C member 1 Homo sapiens 126-138 1751780-9 1991 Furthermore, the 97-kDa and 58-kDa proteins were found in streptavidin-agarose/biotinylated IL-2 purified receptor preparations and showed high affinity for tyrosine kinase substrate support matrixes. Sepharose 71-78 interleukin 2 Homo sapiens 92-96 1909626-1 1991 The enzyme hyodeoxycholic-acid: UDP-glucuronosyltransferase was purified about 230-fold from a solubilized human liver microsomal preparation utilizing anion-exchange chromatography, ampholyte-displacement chromatography and UDP-hexanolamine--Sepharose affinity chromatography. Sepharose 243-252 UDP glucuronosyltransferase family 2 member B4 Homo sapiens 11-30 1959942-2 1991 In the present study we describe a one-step chromatographic procedure for the purification of the anti-IFN-gamma antibodies from human Ig preparations, using a recombinant IFN-gamma-coupled Sepharose CL4B affinity column. Sepharose 190-199 interferon gamma Homo sapiens 103-112 1959942-2 1991 In the present study we describe a one-step chromatographic procedure for the purification of the anti-IFN-gamma antibodies from human Ig preparations, using a recombinant IFN-gamma-coupled Sepharose CL4B affinity column. Sepharose 190-199 interferon gamma Homo sapiens 172-181 1909351-2 1991 As shown by two-dimensional nonreduced/reduced agarose/polyacrylamide gel electrophoresis, the structure of circulating vWf molecules may deviate from that represented by assemblage of a variable number of identical subunits. Sepharose 47-54 von Willebrand factor Homo sapiens 120-123 1875058-6 1991 Supernatants from melanocyte cultures stimulated with either IL-1 alpha or TNF alpha and separated on a heparin-Sepharose column became positive for neutrophil and monocyte chemotactic activity in a dose- and time-dependent fashion. Sepharose 112-121 interleukin 1 alpha Homo sapiens 61-71 1875058-6 1991 Supernatants from melanocyte cultures stimulated with either IL-1 alpha or TNF alpha and separated on a heparin-Sepharose column became positive for neutrophil and monocyte chemotactic activity in a dose- and time-dependent fashion. Sepharose 112-121 tumor necrosis factor Homo sapiens 75-84 1679120-9 1991 Third, solubilized cyclase activity that bound to calmodulin-Sepharose in a Ca(2+)-dependent manner was stimulated by Gs, which had been partially purified from Aplysia CNS, as well as by Ca2+/calmodulin. Sepharose 61-70 calmodulin 1 Homo sapiens 50-60 1679120-9 1991 Third, solubilized cyclase activity that bound to calmodulin-Sepharose in a Ca(2+)-dependent manner was stimulated by Gs, which had been partially purified from Aplysia CNS, as well as by Ca2+/calmodulin. Sepharose 61-70 calmodulin 1 Homo sapiens 193-203 1960708-2 1991 Secretion collected by Sepharose beads contained IgA, IgG, and IgM. Sepharose 23-32 immunoglobulin heavy chain (V7183 family) Mus musculus 54-57 1960708-3 1991 The method could be made ten-fold more sensitive by using anti-mouse IgG or IgM conjugated to Sepharose beads. Sepharose 94-103 immunoglobulin heavy chain (V7183 family) Mus musculus 69-72 1715024-6 1991 Both GTP and GMP, but not ATP, competed for binding of murine GBP-1 to agarose-immobilized GMP. Sepharose 71-78 guanylate binding protein 2b Mus musculus 62-67 1875942-4 1991 We base this conclusion on the following criteria: (i) a disruption of the IRA1 gene produced a mutant with very low membrane-associated levels of adenylate cyclase activity, (ii) membranes made from these mutants were incapable of binding adenylate cyclase in vitro, (iii) IRA1 antibodies inhibit binding of adenylate cyclase to the membrane, and (iv) IRA1 and adenylate cyclase comigrate on Sepharose 4B. Sepharose 393-402 GTPase-activating protein IRA1 Saccharomyces cerevisiae S288C 75-79 1946316-6 1991 S-Sepharose column analysis of sera showed a small peak of pro-PLA2 and a large peak of PLA2 in sera from patients with severe acute pancreatitis, but a large peak of pro-PLA2 in healthy controls and patients with other diseases. Sepharose 2-11 phospholipase A2 group IB Homo sapiens 63-67 1946316-6 1991 S-Sepharose column analysis of sera showed a small peak of pro-PLA2 and a large peak of PLA2 in sera from patients with severe acute pancreatitis, but a large peak of pro-PLA2 in healthy controls and patients with other diseases. Sepharose 2-11 phospholipase A2 group IB Homo sapiens 88-92 1946316-6 1991 S-Sepharose column analysis of sera showed a small peak of pro-PLA2 and a large peak of PLA2 in sera from patients with severe acute pancreatitis, but a large peak of pro-PLA2 in healthy controls and patients with other diseases. Sepharose 2-11 phospholipase A2 group IB Homo sapiens 88-92 1755003-5 1991 These results show that the anti-Xa activity of some HTGL preparations is neither due to the lipase itself nor to the content of AT III, but suggest, that it could be due to contamination with another protein, which binds to heparin sepharose columns but is removed during ion exchange chromatography. Sepharose 233-242 lipase C, hepatic type Homo sapiens 53-57 1686394-1 1991 In rat hepatocytes; the tumorigenic rat liver cell line ARL-16; and the human hepatoma line, Hep G2, 50% of the total gamma-glutamyl transpeptidase (GGT) activity was bound by a Concanavalin-A Sepharose 4B column, calling for alpha-methylmannoside elution (Peak I). Sepharose 193-202 inactive glutathione hydrolase 2 Homo sapiens 149-152 1831222-10 1991 p68 was identified as a calcium-binding protein by its ability to be solubilized from B lymphocyte membranes by EGTA, a calcium-chelating agent, to bind specifically on phenothiazine-Sepharose in a calcium-dependent interaction, and to be recognized by specific antibodies directed against human p68, a calcium-binding protein of the annexin VI family. Sepharose 183-192 annexin A6 Homo sapiens 0-3 1939090-4 1991 An affinity matrix of C1q tails coupled to Sepharose was used to select C1q-binding proteins from detergent extracts of surface-iodinated human monocytes, polymorphonuclear leukocytes, and the U937 cells. Sepharose 43-52 complement C1q A chain Homo sapiens 72-75 1880428-1 1991 We have modified a standard isolation procedure for C1r and C1s, which employs IgG-Sepharose affinity chromatography followed by DEAE chromatography. Sepharose 83-92 complement C1r Homo sapiens 52-55 1880428-1 1991 We have modified a standard isolation procedure for C1r and C1s, which employs IgG-Sepharose affinity chromatography followed by DEAE chromatography. Sepharose 83-92 complement C1s Homo sapiens 60-63 1937496-4 1991 P57 proteinase eluted from MP1-sepharose was inhibited by 5 x 10(-4) M PMSF, enhanced by 0.5% SDS and generated C3 fragments identical to those generated by membrane crude extract of human erythrocytes. Sepharose 31-40 cyclin dependent kinase inhibitor 1C Homo sapiens 0-3 1872403-2 1991 BH-renin was composed mainly (80%) of a form that bound to concanavalin A-agarose (CB-renin). Sepharose 74-81 renin Homo sapiens 3-8 1872403-2 1991 BH-renin was composed mainly (80%) of a form that bound to concanavalin A-agarose (CB-renin). Sepharose 74-81 renin Homo sapiens 86-91 1868610-3 1991 Agarose gel electrophoresis of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes in serum demonstrated markedly increased intestinal isoforms (the "soluble" and the "hydrophobic" forms), which accounted for approximately 60% of total ALP activity. Sepharose 0-7 alkaline phosphatase, placental Homo sapiens 31-51 1868610-3 1991 Agarose gel electrophoresis of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes in serum demonstrated markedly increased intestinal isoforms (the "soluble" and the "hydrophobic" forms), which accounted for approximately 60% of total ALP activity. Sepharose 0-7 alkaline phosphatase, placental Homo sapiens 53-56 1868610-3 1991 Agarose gel electrophoresis of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes in serum demonstrated markedly increased intestinal isoforms (the "soluble" and the "hydrophobic" forms), which accounted for approximately 60% of total ALP activity. Sepharose 0-7 alkaline phosphatase, placental Homo sapiens 234-237 1937496-4 1991 P57 proteinase eluted from MP1-sepharose was inhibited by 5 x 10(-4) M PMSF, enhanced by 0.5% SDS and generated C3 fragments identical to those generated by membrane crude extract of human erythrocytes. Sepharose 31-40 late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 Homo sapiens 27-30 1937940-3 1991 Fab-containing IA on Sepharose retained 70-90% of the SAC of native Ab-containing IA. Sepharose 21-30 FA complementation group B Homo sapiens 0-3 1932371-5 1991 F5 cells produced two species of MIF that could be separated on a phenyl-Sepharose column. Sepharose 73-82 macrophage migration inhibitory factor Homo sapiens 33-36 2071944-1 1991 Centromere protein-B (CENP-B) was purified from HeLa nuclear extract by using a combination of Q-sepharose ion change and oligonucleotide sepharose column chromatography. Sepharose 97-106 centromere protein B Homo sapiens 0-20 2071944-1 1991 Centromere protein-B (CENP-B) was purified from HeLa nuclear extract by using a combination of Q-sepharose ion change and oligonucleotide sepharose column chromatography. Sepharose 97-106 centromere protein B Homo sapiens 22-28 1650483-3 1991 Recombinant TGF-beta 1 elicited dose-dependent directed migration of neutrophils under agarose that was inhibited in the presence of a neutralizing antibody to TGF-beta 1. Sepharose 87-94 transforming growth factor beta 1 Homo sapiens 12-22 1650483-3 1991 Recombinant TGF-beta 1 elicited dose-dependent directed migration of neutrophils under agarose that was inhibited in the presence of a neutralizing antibody to TGF-beta 1. Sepharose 87-94 transforming growth factor beta 1 Homo sapiens 160-170 1821794-0 1991 Polyamine-linked sepharoses: preparation and application to mammalian spermine synthase. Sepharose 17-27 spermine synthase Homo sapiens 70-87 1821794-1 1991 Seven different polyamine-linked Sepharose derivatives were prepared for the affinity chromatography of spermidine and spermine binding macromolecules: Spermine synthase from rat and hog brain was used as a model protein with a spermidine binding site. Sepharose 33-42 spermine synthase Rattus norvegicus 152-169 1862074-3 1991 This modification resulted in denaturation of apolipoprotein AI on SDS/PAGE and increased the negative charge on agarose gel electrophoresis. Sepharose 113-120 apolipoprotein A1 Homo sapiens 46-63 1920880-3 1991 After agarose gel-immunofixation electrophoresis using anti-apo C-II or anti-apo C-III antisera, the immune complexes of apo C-II or apo C-III selectively remained in the gels by salting out the non-immunoreacted proteins and were stained to be measured by the densitometry. Sepharose 6-13 apolipoprotein C3 Homo sapiens 125-142 1713213-5 1991 The hybrid GST-Pim-1 fusion protein was affinity purified on a glutathione-Sepharose column prior to treatment with thrombin for cleavage of the Pim-1 protein from the transferase. Sepharose 75-84 glutathione S-transferase kappa 1 Homo sapiens 11-14 1713213-5 1991 The hybrid GST-Pim-1 fusion protein was affinity purified on a glutathione-Sepharose column prior to treatment with thrombin for cleavage of the Pim-1 protein from the transferase. Sepharose 75-84 Pim-1 proto-oncogene, serine/threonine kinase Homo sapiens 15-20 1715234-3 1991 The angiogenic activity in the RMT-1 conditioned medium was separated into two fractions on a column of heparin-Sepharose; one was eluted with 0.1 M NaCl and the other with 0.5 M NaCl, which are referred to hereafter as rAF-1 and rAF-2, respectively. Sepharose 112-121 small integral membrane protein 38 Rattus norvegicus 31-36 1856198-6 1991 A single radiolabeled component (120 kDa) was identified and shown to be identical with the alpha subunit of GPIIb based on two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western blotting with anti-GPIIb monoclonal antibodies, by isoelectric focusing (pI 4.5-5.5), by immunoaffinity adsorption using monoclonal anti-GPIIb/IIIa antibodies coupled to Sepharose, and by crossed immunoelectrophoresis. Sepharose 388-397 integrin subunit alpha 2b Homo sapiens 109-114 1865126-9 1991 U-EPX was purified by a two step procedure involving affinity chromatography on heparin Sepharose and size exclusion chromatography on Sephadex G-50 superfine. Sepharose 88-97 eosinophil peroxidase Homo sapiens 2-5 1865126-10 1991 Extracted EPX and U-EPX had ribonuclease activity and comigrated on agarose electrophoresis. Sepharose 68-75 eosinophil peroxidase Homo sapiens 10-13 1865126-10 1991 Extracted EPX and U-EPX had ribonuclease activity and comigrated on agarose electrophoresis. Sepharose 68-75 eosinophil peroxidase Homo sapiens 20-23 1854718-8 1991 The cementum mitogenic factor eluting with 2.0 M NaCl from a heparin-Sepharose column was shown to be basic fibroblast growth factor (bFGF) on the basis of inhibition by anti-bFGF antibody and Western blots. Sepharose 69-78 fibroblast growth factor 2 Bos taurus 102-132 1854718-8 1991 The cementum mitogenic factor eluting with 2.0 M NaCl from a heparin-Sepharose column was shown to be basic fibroblast growth factor (bFGF) on the basis of inhibition by anti-bFGF antibody and Western blots. Sepharose 69-78 fibroblast growth factor 2 Bos taurus 134-138 1712728-10 1991 Native tenascin and all fragments containing the distal part of its arms bind to heparin-agarose, whereas the proximal fragments do not. Sepharose 89-96 avian tenascin X Gallus gallus 7-15 1722741-0 1991 Agarose gel electrophoresis of glucose-6-phosphate-dehydrogenase isoenzymes. Sepharose 0-7 glucose-6-phosphate dehydrogenase Homo sapiens 31-64 1859377-1 1991 Isolation of mouse glutathione S-transferase heterodimers by gradient elution of the glutathione-Sepharose affinity matrix. Sepharose 97-106 hematopoietic prostaglandin D synthase Mus musculus 19-44 1906465-2 1991 The nascent transferrin receptor containing core-glycosylated asparagine-linked oligosaccharides does not possess complete intersubunit disulfide bonds, sediments predominantly as a monomer in sucrose density gradients, and shows reduced binding to transferrin-agarose. Sepharose 261-268 transferrin receptor Homo sapiens 12-32 1906465-2 1991 The nascent transferrin receptor containing core-glycosylated asparagine-linked oligosaccharides does not possess complete intersubunit disulfide bonds, sediments predominantly as a monomer in sucrose density gradients, and shows reduced binding to transferrin-agarose. Sepharose 261-268 transferrin Homo sapiens 12-23 2068099-2 1991 To dissect the epitope specificity of the group-specific neutralizing antibodies, CD4 attachment site-specific antibodies (CD4-site Abs) were isolated from total anti-gp120 Abs by using a CD4-blocked gp120SF2-Sepharose column. Sepharose 209-218 CD4 molecule Homo sapiens 123-126 2069550-3 1991 A simple method for isolation of hsp70 binding proteins using hsp70-sepharose column chromatography is described. Sepharose 68-77 heat shock 70 kDa protein 1B Bos taurus 33-38 2069550-3 1991 A simple method for isolation of hsp70 binding proteins using hsp70-sepharose column chromatography is described. Sepharose 68-77 heat shock 70 kDa protein 1B Bos taurus 62-67 2068099-2 1991 To dissect the epitope specificity of the group-specific neutralizing antibodies, CD4 attachment site-specific antibodies (CD4-site Abs) were isolated from total anti-gp120 Abs by using a CD4-blocked gp120SF2-Sepharose column. Sepharose 209-218 CD4 molecule Homo sapiens 123-126 1718450-6 1991 Monoclonal antibody 3D12F11 (subclass IgG1) showed a high affinity for the apoE (Kd = 3.5 +/- 0.5 nM) without any effect on the apoprotein binding to heparin-Sepharose and apoE-induced destruction of dipalmitoylphosphatidylcholine liposomes. Sepharose 158-167 apolipoprotein E Homo sapiens 75-79 1905717-2 1991 GTP cyclohydrolase I, an enzyme that catalyzes the first reaction in the pathway for the biosynthesis of pterin compounds, was purified from of C3H mouse liver by 192-fold to apparent homogeneity, using Ultrogel AcA34, DEAE-Trisacryl, and GTP-agarose gels. Sepharose 243-250 GTP cyclohydrolase 1 Mus musculus 0-20 2066564-7 1991 The numbers of IL-1-produced colonies resulting from IL-1-producing monocytes could be completely abolished by incorporation of rabbit anti-human IL-1 in the semisolid agarose but not by rabbit anti-human IL-6 or anti-human TNF-alpha. Sepharose 168-175 interleukin 1 alpha Homo sapiens 15-19 2066564-7 1991 The numbers of IL-1-produced colonies resulting from IL-1-producing monocytes could be completely abolished by incorporation of rabbit anti-human IL-1 in the semisolid agarose but not by rabbit anti-human IL-6 or anti-human TNF-alpha. Sepharose 168-175 interleukin 1 alpha Homo sapiens 53-57 2066564-7 1991 The numbers of IL-1-produced colonies resulting from IL-1-producing monocytes could be completely abolished by incorporation of rabbit anti-human IL-1 in the semisolid agarose but not by rabbit anti-human IL-6 or anti-human TNF-alpha. Sepharose 168-175 interleukin 1 alpha Homo sapiens 53-57 2065032-9 1991 Analysis of the d less than 1.21 g/ml peritoneal lipoproteins isolated by ultracentrifugation revealed the presence, in both normal and hypercholesterolemic rats, of apolipoprotein A-I-rich lipid complexes with pre-beta mobility on agarose gel electrophoresis. Sepharose 232-239 apolipoprotein A1 Rattus norvegicus 166-184 2065676-7 1991 MCP activity from virally infected L929 cells was concentrated and purified by sequential adsorption to controlled pore glass, heparin-Sepharose chromatography, ion-exchange FPLC and reversed-phase HPLC. Sepharose 135-144 complement component (3b/4b) receptor 1-like Mus musculus 0-3 1893088-1 1991 Monoclonal antibodies against rat liver mitochondrial phospholipase A2 were found to be cross-reactive with the phospholipase A2 present in caseinate-induced rat peritoneal exudate, both in dot-blot and in monoclonal antibody-Sepharose binding experiments. Sepharose 226-235 phospholipase A2 group IB Rattus norvegicus 54-70 1939020-1 1991 A beta-galactosidase was extracted from the internal organs of a sea squirt, Styela plicata, and purified 959-fold, with an 18% yield, by successive gel chromatography, anion-exchange chromatography, chromatofocusing, and affinity chromatography on a Con A-Sepharose column. Sepharose 257-266 galactosidase beta 1 Bos taurus 2-20 1893088-1 1991 Monoclonal antibodies against rat liver mitochondrial phospholipase A2 were found to be cross-reactive with the phospholipase A2 present in caseinate-induced rat peritoneal exudate, both in dot-blot and in monoclonal antibody-Sepharose binding experiments. Sepharose 226-235 phospholipase A2 group IB Rattus norvegicus 112-128 1861869-9 1991 However, compared to control cells, clones expressing N-myc grew in agarose 2.8- to 18-fold higher in response to basic fibroblast growth factor (bFGF) and 5.5- to 55-fold higher in response to platelet-derived growth factor B-chain homodimer (PDGF-BB). Sepharose 68-75 MYCN proto-oncogene, bHLH transcription factor Homo sapiens 54-59 1656060-2 1991 The muM and mM Ca(2+)-sensitive (mu- and mCANP) forms of CANP were separated by DEAE and phenyl Sepharose column chromatography, the latter step enabling removal of the endogenous inhibitor calpastatin. Sepharose 96-105 latexin Homo sapiens 4-7 1716946-8 1991 Removal of excessive IGFBP from patient sera by affinity chromatography on an IGF-II Sepharose column resulted in a significant increase in somatomedin bioactivity. Sepharose 85-94 insulin like growth factor 2 Homo sapiens 78-84 1949232-9 1991 A proteolytically modified form of tyrosyl-tRNA-synthetase possesses, like the main form, the affinity to high-molecular rRNA but it is eluted from the column filled with rRNA-sepharose at lower salt concentration (50 mM KCl) as compared to the main form of the enzyme (100 mM KCl). Sepharose 176-185 tyrosyl-tRNA synthetase 1 Bos taurus 35-58 1682045-7 1991 Conversely, beta-D-[125I]GlcNAc47-BSA bound specifically to gp 160-Sepharose. Sepharose 67-76 glutamyl aminopeptidase Homo sapiens 60-66 1652440-5 1991 Purified antibodies specific for the sequence SAEQNRMGQ (residues 314-322) of rat heart CX43 were covalently bound to a protein-A-Sepharose-CL-4B matrix. Sepharose 130-139 gap junction protein, alpha 1 Rattus norvegicus 88-92 1933942-1 1991 A lectin from Japanese jack bean (Canavalia gladiata agglutinin, CGA) was purified by affinity chromatography on a maltamyl-Sepharose column. Sepharose 124-133 chromogranin A Homo sapiens 65-68 2050694-4 1991 The valyl-tRNA synthetase.EF-1 complex has been purified by gel filtration and tRNA-Sepharose chromatography from 32P-labeled rabbit reticulocytes stimulated by phorbol 12-myristate 13-acetate (PMA) and compared to the complex purified from control cells. Sepharose 84-93 valine--tRNA ligase Oryctolagus cuniculus 4-25 2050696-8 1991 The apoE3 dimer displayed a preference for high density lipoproteins, as determined by agarose chromatography of E3/3 plasma but was stripped from high density lipoproteins by ultracentrifugation. Sepharose 87-94 apolipoprotein E Homo sapiens 4-9 2059225-1 1991 Clusterin was purified from human serum by sequential affinity chromatography over IgG-, protein A- and Con A-Sepharose. Sepharose 110-119 clusterin Oryctolagus cuniculus 0-9 1647974-6 1991 TIMP was co-purified with gelatinase on gelatin sepharose and identified by microsequencing. Sepharose 48-57 TIMP metallopeptidase inhibitor 1 Homo sapiens 0-4 1645708-7 1991 Solubilized PYY receptors bound specifically to concanavalin A-, wheat germ agglutinin-, and soybean-coupled Sepharose, supporting their glycoproteic nature. Sepharose 109-118 peptide YY Rattus norvegicus 12-15 1904058-5 1991 Free clusterin immobilized on the antibody-Sepharose selectively retains apoA-I from total human plasma. Sepharose 43-52 clusterin Homo sapiens 5-14 1904058-5 1991 Free clusterin immobilized on the antibody-Sepharose selectively retains apoA-I from total human plasma. Sepharose 43-52 apolipoprotein A1 Homo sapiens 73-79 1646266-10 1991 Chromatography of the mucin on immunoaffinity columns (MAbs H(13.3), M(33.3) and CCK 061 conjugated to CNBr-activated Sepharose 4B), followed by ELISA and EITB analyses, established the mucin species recognized by the antibodies. Sepharose 118-127 LOC100508689 Homo sapiens 22-27 1648405-13 1991 One of the isozymes of AdoMet synthetase has been purified (1529-fold) to electrophoretic homogeneity by resorting to phenyl Sepharose and ATP Sepharose affinity chromatography. Sepharose 125-134 S-adenosylmethionine synthase Triticum aestivum 23-40 1647180-6 1991 Using the polymerase chain reaction and reverse transcriptase to reverse-transcribe mRNA from mononuclear phagocytes, specific IGF-II/M6P receptor cDNA was amplified and detected by agarose gel electrophoresis from both AM and blood monocytes. Sepharose 182-189 insulin like growth factor 2 Homo sapiens 127-133 1647755-6 1991 A protein of relative molecular mass (Mr) 150 kd was purified from synovial fluid by affinity chromatography on myeloperoxidase-Sepharose. Sepharose 128-137 myeloperoxidase Homo sapiens 112-127 2049072-13 1991 After coupling to Sepharose, the antibody was able to bind to cytochrome P-450(26) from liver as well as from kidney mitochondria and to immunoprecipitate the 26-hydroxylase activity towards 25-hydroxyvitamin D3 and cholesterol when assayed in a reconstituted system. Sepharose 18-27 cytochrome P450 family 2 subfamily D member 6 Sus scrofa 62-78 1910580-2 1991 Dog polymorphonuclear leukocyte cathepsin G was isolated from a granule extract using a two-step procedure including affinity chromatography on a Trasylol-Sepharose gel and ion-exchange chromatography on a CM 52 column. Sepharose 155-164 cathepsin G Homo sapiens 32-43 1934169-2 1991 Based on the substrate specificities of the enzyme preparation obtained by anion exchange chromatography and 3"-phosphoadenosine 5"-phosphate (PAP)-agarose affinity chromatography, AD-ST was supposed to be among isoenzymes of hydroxysteroid STs. Sepharose 148-155 sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 6 Rattus norvegicus 181-186 1912089-5 1991 A rapid differentiation between the two alleles is achieved by agarose gel electrophoresis of the ScaI-digested PCR product. Sepharose 63-70 suppressor of cancer cell invasion Homo sapiens 98-102 1786970-5 1991 More than 95% of metastatic serum thyroglobulin could be bound to concanavalin-A sepharose and be eluted with 0.5 M alpha-methyl mannoside. Sepharose 81-90 thyroglobulin Homo sapiens 34-47 1645756-6 1991 Kinase activity, determined with receptors immobilized on insulin agarose beads, was measured at 0.5 microM ATP, with 1 mg/ml histone, followed by SDS-PAGE. Sepharose 66-73 insulin Homo sapiens 58-65 1939013-1 1991 Rat plasma glutathione peroxidase (GSH-Px) was purified 1,400-fold from rat serum by a combination of phenyl Sepharose, DEAE Sephacel, blue Sepharose and Sephacryl S-200 column chromatographies. Sepharose 109-118 glutathione peroxidase 1 Rattus norvegicus 35-41 1675787-1 1991 Guanylate cyclase from rod photoreceptors of amphibian (toad, Bufo marinus, and frog, Rana catesbeiana) and bovine retinas was solubilized and purified by a single chromatography step on a GTP-agarose column. Sepharose 193-200 guanylate cyclase Bos taurus 0-17 1675787-4 1991 Specific binding to Con A-Sepharose suggested that rod guanylate cyclase is a glycoprotein. Sepharose 26-35 guanylate cyclase Bos taurus 55-72 2052581-1 1991 Using a recombinant rsk gene product as a substrate for in vitro kinase assays, we have identified two mitogen-activated Swiss 3T3 RSK protein kinase activities (referred to as RSK kinase I and RSK kinase II, based on their order of elution from phenyl-Sepharose). Sepharose 253-262 ribosomal protein S6 kinase polypeptide 1 Mus musculus 20-23 2052581-1 1991 Using a recombinant rsk gene product as a substrate for in vitro kinase assays, we have identified two mitogen-activated Swiss 3T3 RSK protein kinase activities (referred to as RSK kinase I and RSK kinase II, based on their order of elution from phenyl-Sepharose). Sepharose 253-262 ribosomal protein S6 kinase polypeptide 1 Mus musculus 131-134 2052581-1 1991 Using a recombinant rsk gene product as a substrate for in vitro kinase assays, we have identified two mitogen-activated Swiss 3T3 RSK protein kinase activities (referred to as RSK kinase I and RSK kinase II, based on their order of elution from phenyl-Sepharose). Sepharose 253-262 ribosomal protein S6 kinase polypeptide 1 Mus musculus 177-180 2052581-1 1991 Using a recombinant rsk gene product as a substrate for in vitro kinase assays, we have identified two mitogen-activated Swiss 3T3 RSK protein kinase activities (referred to as RSK kinase I and RSK kinase II, based on their order of elution from phenyl-Sepharose). Sepharose 253-262 ribosomal protein S6 kinase polypeptide 1 Mus musculus 177-180 2040287-4 1991 The recombinant CKII alpha subunit was purified by DEAE-cellulose chromatography, followed by phosphocellulose and heparin-agarose chromatography. Sepharose 123-130 casein kinase 2 alpha 2 Homo sapiens 16-26 1710253-6 1991 A Sepharose-peptide immunoadsorbent column was examined for its capacity to purify several of these anti-mucin antibodies and it was determined that this procedure was highly efficient--purified IgG and IgM antibodies could be isolated from either hybridoma tissue culture supernatants or ascitic fluids. Sepharose 2-11 LOC100508689 Homo sapiens 105-110 1897991-7 1991 Affinity purification of the antibodies on tg-MLCK Sepharose resulted in the preparation of two fractions of antibodies with different reactivity toward these proteins. Sepharose 51-60 myosin light chain kinase, smooth muscle Ovis aries 46-50 2029579-4 1991 When AT-III was isolated from the plasma of the propositus by heparin-Sepharose chromatography, it had identical mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to normal plasma-derived AT-III, under both reducing and nonreducing conditions. Sepharose 70-79 serpin family C member 1 Homo sapiens 5-11 2029579-5 1991 However, the AT-III-Hamilton species, separated from the propositus" normal AT-III by a combination of heparin-Sepharose and thrombin-Sepharose chromatography, had increased mobility on reductive SDS-PAGE compared with AT-III from the propositus isolated by heparin-Sepharose chromatography alone. Sepharose 111-120 serpin family C member 1 Homo sapiens 13-19 2029579-5 1991 However, the AT-III-Hamilton species, separated from the propositus" normal AT-III by a combination of heparin-Sepharose and thrombin-Sepharose chromatography, had increased mobility on reductive SDS-PAGE compared with AT-III from the propositus isolated by heparin-Sepharose chromatography alone. Sepharose 134-143 serpin family C member 1 Homo sapiens 13-19 2029579-5 1991 However, the AT-III-Hamilton species, separated from the propositus" normal AT-III by a combination of heparin-Sepharose and thrombin-Sepharose chromatography, had increased mobility on reductive SDS-PAGE compared with AT-III from the propositus isolated by heparin-Sepharose chromatography alone. Sepharose 134-143 coagulation factor II, thrombin Homo sapiens 125-133 2029579-5 1991 However, the AT-III-Hamilton species, separated from the propositus" normal AT-III by a combination of heparin-Sepharose and thrombin-Sepharose chromatography, had increased mobility on reductive SDS-PAGE compared with AT-III from the propositus isolated by heparin-Sepharose chromatography alone. Sepharose 134-143 serpin family C member 1 Homo sapiens 13-19 2029579-5 1991 However, the AT-III-Hamilton species, separated from the propositus" normal AT-III by a combination of heparin-Sepharose and thrombin-Sepharose chromatography, had increased mobility on reductive SDS-PAGE compared with AT-III from the propositus isolated by heparin-Sepharose chromatography alone. Sepharose 134-143 coagulation factor II, thrombin Homo sapiens 125-133 2029579-6 1991 Under nonreducing conditions this AT-III-Hamilton species had decreased mobility compared with AT-III from the propositus (or normal AT-III) isolated only by heparin-Sepharose chromatography. Sepharose 166-175 serpin family C member 1 Homo sapiens 34-40 2029579-8 1991 Approximately 50% of the AT-III from the propositus isolated by heparin-Sepharose chromatography, when incubated with either human alpha-thrombin or factor Xa, did not form complex but was cleaved, presumably at the reactive center Arg393-Ser394. Sepharose 72-81 serpin family C member 1 Homo sapiens 25-31 1850386-2 1991 OFA was purified from the amniotic fluids by ammonium sulfate precipitation at 30-70% saturation, followed by successive gel chromatography of the OFA-containing fraction on Sephacryl-S300 HR, Q- and S-Sepharoses and lentil lectin agarose. Sepharose 202-212 oncofetal antigen Mus musculus 0-3 1850386-2 1991 OFA was purified from the amniotic fluids by ammonium sulfate precipitation at 30-70% saturation, followed by successive gel chromatography of the OFA-containing fraction on Sephacryl-S300 HR, Q- and S-Sepharoses and lentil lectin agarose. Sepharose 231-238 oncofetal antigen Mus musculus 0-3 1654785-4 1991 The solubilized receptor was further purified by TNF-Sepharose prepared by coupling of TNF to tresyl-activated Sepharose 4B. Sepharose 53-62 tumor necrosis factor Homo sapiens 49-52 1654785-4 1991 The solubilized receptor was further purified by TNF-Sepharose prepared by coupling of TNF to tresyl-activated Sepharose 4B. Sepharose 53-62 tumor necrosis factor Homo sapiens 87-90 1654785-6 1991 Furthermore, the TNF-Sepharose-bound fraction demonstrated GTP gamma S binding and GTPase activity. Sepharose 21-30 tumor necrosis factor Homo sapiens 17-20 1654792-4 1991 The purified TyrS-binding protein was found to bind concanavalin A-Sepharose and yielded a positive reaction toward periodic acid-Schiff (PAS) staining, indicating its glycoprotein nature. Sepharose 67-76 tyrosyl-tRNA synthetase 1 Bos taurus 13-17 1654792-5 1991 The purified TyrS-binding protein displayed strong binding to TyrS, but not the unmodified tyrosine, covalently bonded to Sepharose. Sepharose 122-131 tyrosyl-tRNA synthetase 1 Bos taurus 13-17 1897961-11 1991 Binding of gp 80 to heparin-agarose or fibrin-Sepharose, however, was inhibited in the presence of added fibronectin or the monoclonal antibody. Sepharose 28-35 clusterin Canis lupus familiaris 11-16 1897961-11 1991 Binding of gp 80 to heparin-agarose or fibrin-Sepharose, however, was inhibited in the presence of added fibronectin or the monoclonal antibody. Sepharose 28-35 fibronectin 1 Canis lupus familiaris 105-116 1897961-11 1991 Binding of gp 80 to heparin-agarose or fibrin-Sepharose, however, was inhibited in the presence of added fibronectin or the monoclonal antibody. Sepharose 46-55 clusterin Canis lupus familiaris 11-16 1897961-11 1991 Binding of gp 80 to heparin-agarose or fibrin-Sepharose, however, was inhibited in the presence of added fibronectin or the monoclonal antibody. Sepharose 46-55 fibronectin 1 Canis lupus familiaris 105-116 1897964-1 1991 Human and rat intestinal mucin was purified by equilibrium density gradient centrifugation and Sepharose 2B chromatography according to M. Mantle, D. Mantle, and A. Allen (1981, Biochem. Sepharose 95-104 solute carrier family 13 member 2 Rattus norvegicus 25-30 2039449-6 1991 Recombinant prorenin was bound to a Cibacron Blue-Sepharose column and eluted with 1.4 M-NaCl, but was not retained by an octapeptide renin inhibitor (H-77)-Sepharose column. Sepharose 50-59 renin Rattus norvegicus 15-20 2039449-7 1991 Trypsin activation of prorenin increased the renin activity 110-fold, caused binding to an H-77-Sepharose column and nullified the reactivity to the above two kinds of anti-prosegment antibodies, findings indicating that the activation of prorenin with trypsin is due to the cleavage of the prosegment. Sepharose 96-105 renin Rattus norvegicus 25-30 1706970-10 1991 When Fc gamma RIII, a complex sialoglycoprotein consisting of almost 50% oligosaccharides, was immunoprecipitated from neutrophils with 3G8 Fab Sepharose and subsequently digested with N-glycanase, 5 of the 6 mAb were capable of immunoprecipitating a deglycosylated polypeptide migrating at 29 kDa. Sepharose 144-153 Fc gamma receptor IIIa Homo sapiens 5-18 2065480-7 1991 In this way renin mRNA was detected in kidney and several extrarenal tissues as a PCR product of approximately 330 base pairs on agarose gels by ethidium-bromide staining or hybridization probing. Sepharose 129-136 renin Homo sapiens 12-17 1935879-1 1991 Thin-layer isoelectric focusing in agarose within the pH range of 4.0-6.5 has shown a high resolution of the soluble cytoplasmic glutamate-oxaloacetate transaminase (GOT1) banding pattern. Sepharose 35-42 glutamic-oxaloacetic transaminase 1 Homo sapiens 166-170 1710230-6 1991 The mitogens extracted from cell lysates and from the ECM are closely related to aFGF or bFGF by the criteria that they bind to heparin-sepharose and elute at 1.1 M (aFGF) or 1.5 M (bFGF) NaCl, have molecular weights of about 18,000, and react with anti-aFGF (1.1 M), or anti-bFGF (1.5 M) antibodies when analyzed by Western blots and by radioimmunoassay specific for aFGF and bFGF. Sepharose 136-145 fibroblast growth factor 1 Bos taurus 81-85 1710230-6 1991 The mitogens extracted from cell lysates and from the ECM are closely related to aFGF or bFGF by the criteria that they bind to heparin-sepharose and elute at 1.1 M (aFGF) or 1.5 M (bFGF) NaCl, have molecular weights of about 18,000, and react with anti-aFGF (1.1 M), or anti-bFGF (1.5 M) antibodies when analyzed by Western blots and by radioimmunoassay specific for aFGF and bFGF. Sepharose 136-145 fibroblast growth factor 2 Bos taurus 89-93 1880335-1 1991 An affinity chromatography column packed with nonporous agarose beads derivatized with human growth hormone via carbonyldiimidazol was used for the purification of antibodies against human growth hormone from antiserum. Sepharose 56-63 growth hormone 1 Homo sapiens 189-203 2016539-6 1991 Substrate-affinity chromatography with phe-Sepharose or FMLP-Sepharose provided partial purification of enzyme activity among Mr 65,000 to 70,000 FMLP- or phe-binding proteins. Sepharose 43-52 formyl peptide receptor 1 Homo sapiens 146-150 2016539-6 1991 Substrate-affinity chromatography with phe-Sepharose or FMLP-Sepharose provided partial purification of enzyme activity among Mr 65,000 to 70,000 FMLP- or phe-binding proteins. Sepharose 61-70 formyl peptide receptor 1 Homo sapiens 56-60 2016539-6 1991 Substrate-affinity chromatography with phe-Sepharose or FMLP-Sepharose provided partial purification of enzyme activity among Mr 65,000 to 70,000 FMLP- or phe-binding proteins. Sepharose 61-70 formyl peptide receptor 1 Homo sapiens 146-150 2072046-0 1991 Rapid apolipoprotein E phenotyping by immunofixation in agarose. Sepharose 56-63 apolipoprotein E Homo sapiens 6-22 1944260-2 1991 Tyrosyl-tRNA synthetase from bovine liver (Mr 2.59 kDa) was fully retained on the rRNA-Sepharose and eluted by buffer with 100 mM KCl. Sepharose 87-96 tyrosyl-tRNA synthetase 1 Bos taurus 0-23 1866126-4 1991 Vitellogenin was isolated from the plasma of oestradiol-treated cockerels, and the antibody to it elicited in rabbits and made vitellogenin-specific by affinity chromatography on lipovitellin-Sepharose columns. Sepharose 192-201 vitellogenin Oryctolagus cuniculus 0-12 1850005-9 1991 Poly(A)- LAT cochromatographed with uncapped rRNA on m-aminophenyl boronate agarose under conditions in which capped mRNA was bound. Sepharose 76-83 linker for activation of T cells Homo sapiens 9-12 1685265-6 1991 This allowed HLA-DRB3 typing on agarose gel by determining whether the amplification product has been digested or not. Sepharose 32-39 major histocompatibility complex, class II, DR beta 3 Homo sapiens 13-21 1717176-6 1991 From P30 to adulthood a constant value of 2.5 ng/mg was measured, aFGF content in the different brain extracts was further characterized by its affinity for heparin-Sepharose, its elution at 1 M NaCl from this column and its capacity to induce thymidine incorporation in quiescent fibroblasts. Sepharose 165-174 fibroblast growth factor 1 Mus musculus 66-70 2019459-2 1991 Immunoblotting of eluates from whole-cell extracts applied to LM Sepharose indicates the presence of an LM-binding protein of 116-130 kDa that reacted with an anti-beta 1 integrin antibody, suggesting that the putative LM receptor of MEL-85 cells is a member of the integrin family. Sepharose 65-74 integrin subunit beta 1 Homo sapiens 164-179 2026258-2 1991 This method takes advantage of the fact that the protein remains soluble in 2.5% perchloric acid (PCA) and that it binds to a calmodulin-Sepharose column in the absence of calcium: Other PKC substrate proteins that remain to be identified were also found to share these two properties, suggesting that a class of calmodulin-binding PKC substrates may exist in the brain. Sepharose 137-146 calmodulin Bos taurus 126-136 2031941-1 1991 A phospholipase A2 was purified from rabbit platelet cytosolic fraction to near homogeneity by sequential column chromatographies on heparin-Sepharose, DEAE-Sephacel, butyl-Toyopearl, DEAE-5PW ion-exchange HPLC, and TSK gel G3000SW gel-filtration HPLC. Sepharose 141-150 phospholipase A2 Oryctolagus cuniculus 2-18 2018799-7 1991 Competitive binding experiments with agarose-bound heparin and soluble GAG also suggest that after formation of insoluble complexes with arterial CSPG and resolubilization the exposure of Lys, Arg-rich segments of apoB-100 is increased. Sepharose 37-44 apolipoprotein B Homo sapiens 214-222 1707917-2 1991 Stimulation of human normodense eosinophils with IgG- or secretory IgA (sIgA)-coated Sepharose beads induced cellular degranulation, as measured by the release of the granule protein, eosinophil-derived neurotoxin (EDN). Sepharose 85-94 ribonuclease A family member 2 Homo sapiens 184-213 2025229-7 1991 During the purification of GSTs with the use of affinity chromatography on GSH linked to epoxy-activated Sepharose 6B, FAEES and GST activities from each of these tissues segregated independently. Sepharose 105-114 glutathione S-transferase kappa 1 Homo sapiens 27-31 2025229-7 1991 During the purification of GSTs with the use of affinity chromatography on GSH linked to epoxy-activated Sepharose 6B, FAEES and GST activities from each of these tissues segregated independently. Sepharose 105-114 glutathione S-transferase kappa 1 Homo sapiens 27-30 1901881-6 1991 A strong affinity of TSTGF and IL-7 for heparin was confirmed by the fact that both TSTGF and IL-7 adhered to columns of heparin-agarose and were eluted by salt. Sepharose 129-136 interleukin 7 Homo sapiens 31-35 1901881-6 1991 A strong affinity of TSTGF and IL-7 for heparin was confirmed by the fact that both TSTGF and IL-7 adhered to columns of heparin-agarose and were eluted by salt. Sepharose 129-136 interleukin 7 Homo sapiens 94-98 2016290-7 1991 Chromatography on antithrombin III-Sepharose gel indicated that the structural change involved in ST heparin resulted in an obvious increase in the ability to bind antithrombin III. Sepharose 35-44 serpin family C member 1 Homo sapiens 18-34 2016290-7 1991 Chromatography on antithrombin III-Sepharose gel indicated that the structural change involved in ST heparin resulted in an obvious increase in the ability to bind antithrombin III. Sepharose 35-44 serpin family C member 1 Homo sapiens 164-180 2015821-1 1991 alpha 1-Antichymotrypsin purified from normal human serum was separated by affinity chromatography into th ree microheterogeneous forms on a concanavalin-A-Sepharose column: a pass-through (peak 1), a retarded (peak 2) and a bound form (peaks 3 + 4). Sepharose 156-165 serpin family A member 3 Homo sapiens 0-24 2057915-1 1991 Chemical modification of tryptophan residues in antithrombin III by dimethyl (2-hydroxy-5-nitrobenzyl) sulfonium bromide (HNBSB) generates products with similar levels of modification (equivalent to 0.9 mole 2-hydroxy-5-nitrobenzyl [HNB] incorporated/mole of antithrombin III) but with high or low affinity for heparin-Sepharose. Sepharose 319-328 serpin family C member 1 Homo sapiens 48-64 1904673-2 1991 Nineteen patients presented free kappa or lambda light chain bands in CSF after agarose isoelectric focusing, protein transfer to nitrocellulose and immunostaining with specific antisera. Sepharose 80-87 colony stimulating factor 2 Homo sapiens 70-73 1849357-3 1991 Here we demonstrate the purification of HGF from human placenta with heparin-agarose chromatography and TSK-heparin high-pressure liquid chromatography and describe the distribution of placental HGF by immunohistochemistry using a polyclonal antibody to HGF. Sepharose 77-84 hepatocyte growth factor Homo sapiens 40-43 1897944-2 1991 In the presence of 1 mM reduced glutathione (GSH), the inactivated GST-P (-pi) was effectively reactivated by the action of thioltransferase, which had been partially purified from rat liver by GSH-Sepharose affinity chromatography and gel filtration using Sephadex G-75. Sepharose 198-207 glutathione S-transferase pi 1 Rattus norvegicus 67-72 1905550-3 1991 All 4 monoclonal antibodies inhibited binding of intact t-PA to lysine-Sepharose and fibrin, and they suppressed fibrin-mediated activation of one-chain t-PA-amidolytic activity. Sepharose 71-80 plasminogen activator, tissue type Homo sapiens 56-60 1905550-4 1991 Binding analysis demonstrated that mAB 25 inhibited t-PA binding to lysine-Sepharose and to fibrin as well as fibrin-mediated enhancement of one-chain t-PA-amidolytic activity in a competitive manner with inhibitor constants of 5 nmol/l, 3 nmol/l and 10 nmol/l, respectively. Sepharose 75-84 plasminogen activator, tissue type Homo sapiens 52-56 2018481-6 1991 On employing an affinity gel fractionation technique, it was shown that partially purified TyrS-binding protein exhibited binding affinity towards Sepharose gels covalently bonded to fibronectin or fibrinogen, but not towards Sepharose gels bonded to albumin or transferrin. Sepharose 147-156 fibronectin 1 Bos taurus 183-194 2043278-2 1991 The detection and accumulation of tetrapyrrole intermediates synthesized by the action of bovine liver porphobilinogen deaminase immobilized to Sepharose 4B is reported. Sepharose 144-156 hydroxymethylbilane synthase Bos taurus 103-128 1826890-7 1991 Adsorption of factor H preparations onto anti-factor H-Sepharose completely suppressed the phenomenon. Sepharose 55-64 complement factor H Homo sapiens 14-22 1826890-7 1991 Adsorption of factor H preparations onto anti-factor H-Sepharose completely suppressed the phenomenon. Sepharose 55-64 complement factor H Homo sapiens 46-54 1830003-3 1991 The tip supports the agarose slice and is connected to dialysis tubing. Sepharose 21-28 TOR signaling pathway regulator Homo sapiens 4-7 1849072-4 1991 Translation of mbh1 RNA in rabbit reticulocyte extracts produces an approximately 45 kd protein capable of binding actin-coupled agarose beads in vitro in a Ca2(+)-dependent manner. Sepharose 129-136 capping protein (actin filament), gelsolin-like Mus musculus 15-19 1849072-4 1991 Translation of mbh1 RNA in rabbit reticulocyte extracts produces an approximately 45 kd protein capable of binding actin-coupled agarose beads in vitro in a Ca2(+)-dependent manner. Sepharose 129-136 actin Oryctolagus cuniculus 115-120 1865051-3 1991 or C.I.19, and adsorption of different drugs (neuroleptics and local anesthetics) onto calmodulin immobilized on agarose gel. Sepharose 113-120 calmodulin 1 Homo sapiens 87-97 1648569-5 1991 From these results we selected an appropriate antibody for affinity purification of the non-structural viral protein NS1 on CNBr-activated sepharose columns. Sepharose 139-148 influenza virus NS1A binding protein Homo sapiens 117-120 2051696-8 1991 In the soft-agarose semi-solid medium, myc-cl3 cells formed about 35 times more numerous colonies than T-24 cells. Sepharose 12-19 MYC proto-oncogene, bHLH transcription factor Homo sapiens 39-42 2066390-4 1991 Tf-R was collected by affinity binding to Tf-Sepharose and extracted by consecutive treatment with 4 different kinds of buffers. Sepharose 45-54 transferrin receptor Homo sapiens 0-4 1849269-1 1991 A recombinant deletion mutant of the 155-amino acid form of human basic fibroblast growth factor (bFGF), lacking amino acid residues 27-32 (Lys-Asp-Pro-Lys-Arg-Leu), was expressed in Escherichia coli and purified to homogeneity by heparin-Sepharose affinity chromatography. Sepharose 239-248 fibroblast growth factor 2 Homo sapiens 66-96 1866941-1 1991 A 2.0 kb BamHI-HindIII fragment of pDG0103 from Australia containing gentamicin 2"-o-adenylytransferase [ANT(2")] gene and a 4.9 kb PstI-EcoRI fragment of pBY102 were recovered from low-temperature-melting agarose by the slot method. Sepharose 206-213 phosphoglycerate dehydrogenase Homo sapiens 35-38 2013279-4 1991 Anticyclin immunoprecipitates, extracts purified on specific p34cdc2-binding p13suc1-Sepharose and affinity-purified H1 kinase display strong HMG I, Y and P1 phosphorylating activities, demonstrating that the p34cdc2/cyclincdc13 complex is the active kinase phosphorylating these HMG proteins. Sepharose 85-94 cyclin dependent kinase 1 Homo sapiens 61-68 1706339-5 1991 Antibodies were purified by chromatography on Affi-Gel blue and 2-5A-Sepharose-immobilized RNase L. Sepharose 69-78 ribonuclease L (2', 5'-oligoisoadenylate synthetase-dependent) Mus musculus 91-98 1848551-11 1991 Inhibition by L-proline could be reversed in the binding studies by increasing the amount of apo(a); and L-proline-Sepharose bound plasma Lp(a), suggesting that L-proline acted as a ligand for the kringle-4-like domain(s) of apo(a) involved in the binding of apoB-Lp. Sepharose 115-124 lipoprotein(a) Homo sapiens 138-143 1848780-2 1991 Gel retardation analysis of DNA-Sepharose chromatographic fractions of rat hepatic cytosol indicated that TCDD-dependent and sequence-specific DNA binding coeluted with a 200-kDa form of the Ah receptor (peak 2) previously characterized as being multimeric and having high affinity for calf thymus DNA. Sepharose 32-41 aryl hydrocarbon receptor Rattus norvegicus 191-202 2012597-8 1991 40% of the mucin in partially reduced samples was recovered in the Sepharose 2B void volume, this material contained no 118 kDa glycoprotein and apparently consisted of disulphide-bound mucin monomers. Sepharose 67-76 solute carrier family 13 member 2 Rattus norvegicus 11-16 2012601-8 1991 Cathepsin D-digested pFn applied to a heparin-agarose column and eluted with an NaCl stepwise gradient (0.1 M, 0.25 M and 0.5 M) released two polypeptides (75 kDa and 65 kDa) in the 0.5 M-NaCl peak. Sepharose 46-53 cathepsin D Homo sapiens 0-11 2012606-1 1991 A major collagen-binding heat-shock glycoprotein from L6 myoblasts, designated gp46, was purified by gelatin-agarose chromatography and ion-exchange chromatography. Sepharose 109-116 serpin family H member 1 Homo sapiens 79-83 2012606-2 1991 Purified gp46 was functionally active, as shown by its ability to rebind to gelatin-agarose, and was homogeneous as determined by SDS/polyacrylamide-gel electrophoresis. Sepharose 84-91 serpin family H member 1 Homo sapiens 9-13 1707917-2 1991 Stimulation of human normodense eosinophils with IgG- or secretory IgA (sIgA)-coated Sepharose beads induced cellular degranulation, as measured by the release of the granule protein, eosinophil-derived neurotoxin (EDN). Sepharose 85-94 ribonuclease A family member 2 Homo sapiens 215-218 1996556-3 1991 Low-resolution agarose gel electrophoresis showed a vWF with all size multimers in plasma and platelets. Sepharose 15-22 von Willebrand factor Homo sapiens 52-55 1903618-5 1991 Consumption of the complement by acute-phase serum was depressed when CRP was removed from acute-phase serum by CPS-sepharose 4B affinity chromatography. Sepharose 116-125 c-reactive protein, pentraxin-related Oncorhynchus mykiss 70-73 1897932-1 1991 Homoserine dehydrogenase of Saccharomyces cerevisiae has been rapidly purified to homogeneity by heat and acid treatments, ammonium sulfate fractionation, and chromatography on Matrex Gel Red A and Q-Sepharose columns. Sepharose 200-209 homoserine dehydrogenase Saccharomyces cerevisiae S288C 0-24 1910280-3 1991 In this report, affinity chromatography on benzamidine-Sepharose was investigated for the separation of two-chain from one-chain tPA. Sepharose 55-64 chromosome 20 open reading frame 181 Homo sapiens 129-132 2006918-6 1991 Immobilised proteinase could be rapidly separated from the cleavage products and the released, active phosphatase was purified away from the CRD by re-chromatography on galactose-Sepharose. Sepharose 179-188 endogenous retrovirus group K member 10 Homo sapiens 12-22 2029638-2 1991 The two kynurenine aminotransferases (KATs), arbitrarily termed "KAT I" and "KAT II", could be physically separated by isoelectric focussing on a pH 3-10 Ampholine gradient, and, more completely, by differential elution from a DEAE-Sepharose column. Sepharose 232-241 kynurenine aminotransferase 1 Homo sapiens 65-71 2064630-1 1991 Two soybean insulin-binding proteins were isolated using affinity chromatography on insulin-Sepharose. Sepharose 92-101 insulin Bos taurus 12-19 2064630-1 1991 Two soybean insulin-binding proteins were isolated using affinity chromatography on insulin-Sepharose. Sepharose 92-101 insulin Bos taurus 84-91 1847663-8 1991 The cellular growth response of DiFi cells to exogenous EGF was studied in monolayer cultures as well as in a soft agarose assay. Sepharose 115-122 epidermal growth factor Homo sapiens 56-59 1879335-10 1991 Heparin-sepharose affinity chromatography suggests that the biological activities of CAF probably correspond to the presence of acidic and basic fibroblast growth factor (aFGF and bFGF). Sepharose 8-17 fibroblast growth factor 1 Gallus gallus 171-175 1879335-10 1991 Heparin-sepharose affinity chromatography suggests that the biological activities of CAF probably correspond to the presence of acidic and basic fibroblast growth factor (aFGF and bFGF). Sepharose 8-17 fibroblast growth factor 2 Gallus gallus 180-184 2009912-1 1991 Anti-immunoglobulin (Ig)-activated B lymphoblasts, prepared by culturing high-density B cells with anti-Ig-Sepharose for 48 h, can be induced to secrete IgM and IgG1 by a mixture of T cell-derived lymphokines containing interleukin (IL) 4. Sepharose 107-116 LOC105243590 Mus musculus 161-165 2009912-1 1991 Anti-immunoglobulin (Ig)-activated B lymphoblasts, prepared by culturing high-density B cells with anti-Ig-Sepharose for 48 h, can be induced to secrete IgM and IgG1 by a mixture of T cell-derived lymphokines containing interleukin (IL) 4. Sepharose 107-116 interleukin 4 Homo sapiens 220-238 1900308-5 1991 Fibrin degradation products stimulate fibrinogenolysis by binding t-PA and plasminogen because approximately 70% of the labeled material in the clot lysates binds to both t-PA- and plasminogen-Sepharose, and only the bound fractions have potentiating activity. Sepharose 193-202 plasminogen activator, tissue type Homo sapiens 66-70 1997522-3 1991 Size analysis of the polymerase chain reaction products by agarose gel electrophoresis revealed bands at 0.2 and 0.37 kilobases (kb), sizes predicted to be obtained when the two forms of TPO cDNA are present. Sepharose 59-66 thyroid peroxidase Homo sapiens 187-190 1901361-4 1991 A MAb of the IgG1 subclass was isolated in good yield from the murine ascites by affinity chromatography with recombinant protein G-Sepharose 4B. Sepharose 132-141 immunoglobulin heavy constant gamma 1 (G1m marker) Mus musculus 13-17 2051601-5 1991 After papain or pepsin digestion, western blotting with anti-Fab antiserum revealed that the Fab fraction of IgG-F and IgG-S had identical mobility by agarose electrophoresis. Sepharose 151-158 FA complementation group B Homo sapiens 93-96 1780907-1 1991 Affinity chromatography on heparin-Sepharose has been widely used for the purification of post-heparin plasma triglyceride lipases, but this procedure alone yields lipase fractions with a high content of antithrombin III (AT), which also binds to heparin and coelutes with the lipases. Sepharose 35-44 serpin family C member 1 Homo sapiens 204-220 1671871-3 1991 We have developed a method based on polymerase chain reaction (PCR) amplification of the genomic DNA fragment of interest followed by HindIII endonuclease digestion and agarose gel electrophoresis which permits a more rapid and reliable determination of the CR1 genotype. Sepharose 169-176 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 258-261 1993681-9 1991 Both of the products formed with apoA-I and -A-II migrated slower than plasma high density lipoprotein in electrophoresis on agarose gel. Sepharose 125-132 apolipoprotein A1 Homo sapiens 33-49 1996994-1 1991 The polymerase chain reaction was used to amplify bovine tooth amelogenin cDNA, resulting in several products which were separated by agarose gel electrophoresis. Sepharose 134-141 amelogenin, X isoform Bos taurus 63-73 2053103-6 1991 Peptide alpha 27-50 that was covalently linked to Sepharose bound active site-inhibited thrombin at low ionic strength and was eluted at higher salt concentration. Sepharose 50-59 coagulation factor II, thrombin Homo sapiens 88-96 1846626-5 1991 Insulin receptors were not detectable in either cell type by binding analyses; however, immunoblot analysis revealed insulin receptor alpha-subunits in wheat germ agglutinin-Sepharose-purified membranes from db/db cells. Sepharose 174-183 insulin receptor Mus musculus 117-133 1854184-6 1991 However, SSP added simultaneously, 30 min before or 30 min after PE significantly increased elastin digestion in elastin-agarose plate with alpha 1PI. Sepharose 121-128 elastin Homo sapiens 92-99 1854184-6 1991 However, SSP added simultaneously, 30 min before or 30 min after PE significantly increased elastin digestion in elastin-agarose plate with alpha 1PI. Sepharose 121-128 elastin Homo sapiens 113-120 1650611-5 1991 The glycoprotein nature of the secretin receptor has also been demonstrated using WGA-agarose affinity chromatography of the solubilized 125I-secretin receptor complex. Sepharose 86-93 secretin receptor Rattus norvegicus 31-48 1650611-5 1991 The glycoprotein nature of the secretin receptor has also been demonstrated using WGA-agarose affinity chromatography of the solubilized 125I-secretin receptor complex. Sepharose 86-93 secretin receptor Rattus norvegicus 142-159 1847619-2 1991 Insulin and insulin-like-growth-factor-I (IGF-I) receptors were partially purified from full-grown (stages V-VI) Xenopus laevis oocytes by affinity chromatography on wheat-germ agglutinin-agarose. Sepharose 188-195 insulin like growth factor 1 L homeolog Xenopus laevis 42-47 1873341-1 1991 A protein p26 with molecular weight 26 kDa capable of binding to delipidated rhodopsin immobilized on Concanavalin A-Sepharose was found in photoreceptor cells of bovine retina. Sepharose 117-126 recoverin Bos taurus 10-13 1873341-1 1991 A protein p26 with molecular weight 26 kDa capable of binding to delipidated rhodopsin immobilized on Concanavalin A-Sepharose was found in photoreceptor cells of bovine retina. Sepharose 117-126 rhodopsin Bos taurus 77-86 1988126-6 1991 LPL mRNA from the epididymal fat pad and cardiac muscle migrated to the same site on agarose gel and hybridized to a LPL-specific complementary DNA probe. Sepharose 85-92 lipoprotein lipase Rattus norvegicus 0-3 1988163-5 1991 The induction of the p55 (Tac) subunit of the IL-2R was demonstrable by two-color flow cytometry on a small percentage (5%) of B cells and on a higher percentage (10%) of non-B cells 3 days after exposure to TT-coupled Sepharose. Sepharose 219-228 interleukin 2 receptor subunit alpha Homo sapiens 21-24 1988163-5 1991 The induction of the p55 (Tac) subunit of the IL-2R was demonstrable by two-color flow cytometry on a small percentage (5%) of B cells and on a higher percentage (10%) of non-B cells 3 days after exposure to TT-coupled Sepharose. Sepharose 219-228 interleukin 2 receptor subunit alpha Homo sapiens 46-51 1991350-4 1991 Anti-MLCK Fab was then purified by chromatography on an MLCK-Sepharose 4B column. Sepharose 61-70 myosin light chain kinase, smooth muscle Meleagris gallopavo 5-9 1846580-8 1991 3) When affinity-purified lysates from biosynthetically labeled CEFs were incubated with antiserum to the rat IGF-II/Man 6-P receptor, a 245 kilodalton protein was immunoprecipitated from lysates that had been affinity purified on beta-galactosidase-Sepharose but not after purification on IGF-II-Sepharose. Sepharose 250-259 insulin-like growth factor 2 Rattus norvegicus 110-116 1846580-8 1991 3) When affinity-purified lysates from biosynthetically labeled CEFs were incubated with antiserum to the rat IGF-II/Man 6-P receptor, a 245 kilodalton protein was immunoprecipitated from lysates that had been affinity purified on beta-galactosidase-Sepharose but not after purification on IGF-II-Sepharose. Sepharose 297-306 insulin-like growth factor 2 Rattus norvegicus 110-116 1846580-9 1991 By contrast, a truncated IGF-II/Man 6-P receptor, presumably internalized from the fetal bovine serum used to feed the cells, was purified from lysates of unlabeled CEFs on IGF-II-Sepharose. Sepharose 180-189 insulin like growth factor 2 Homo sapiens 25-31 1846580-9 1991 By contrast, a truncated IGF-II/Man 6-P receptor, presumably internalized from the fetal bovine serum used to feed the cells, was purified from lysates of unlabeled CEFs on IGF-II-Sepharose. Sepharose 180-189 insulin like growth factor 2 Homo sapiens 173-179 1988290-2 1991 Biologically active immunoreactive Mr 18,000 bFGF can be isolated by heparin-Sepharose affinity chromatography from the extract of GM 7372 cell nuclei. Sepharose 77-86 fibroblast growth factor 2 Bos taurus 45-49 1709143-2 1991 We have purified soluble Fc epsilon RII/CD23 from culture supernatants of RPMI-8866 cells on an IgE Sepharose column, and studied its ability to release histamine from human nasal polyp mast cells. Sepharose 100-109 Fc epsilon receptor II Homo sapiens 25-39 2037387-6 1991 The most common biological effects of PP over protein-A sepharose were significant drops in IgG (66% of pre-PP values), CH50 and C3 (73% of pre-PP values) and a significant generation of C3a and C5a anaphylatoxins. Sepharose 56-65 complement C3 Homo sapiens 187-190 2037387-6 1991 The most common biological effects of PP over protein-A sepharose were significant drops in IgG (66% of pre-PP values), CH50 and C3 (73% of pre-PP values) and a significant generation of C3a and C5a anaphylatoxins. Sepharose 56-65 complement C5a receptor 1 Homo sapiens 195-198 1713912-2 1991 One of the antibodies, TPA1-70, strongly inhibited the enzymatic activity of tPA in a fibrin agarose plate assay, while it did not affect the enzymatic activity towards the synthetic substrate or plasminogen. Sepharose 93-100 chromosome 20 open reading frame 181 Homo sapiens 77-80 1851245-1 1991 Gelatinase (matrix metalloproteinase 2) purified from culture medium of MDCK cells by affinity chromatography on gelatin-sepharose was tested against type XI collagen. Sepharose 121-130 matrix metallopeptidase 2 Canis lupus familiaris 12-38 1821773-0 1991 Rapid purification of beta-glucuronidase from human liver by immunoaffinity chromatography employing specific murine monoclonal IgG binding to tresyl-activated agarose. Sepharose 160-167 glucuronidase beta Homo sapiens 22-40 1703436-6 1991 At pH 7.4, PAI-1 quantitatively binds to heparin-Sepharose and can be eluted with increasing [NaCl]. Sepharose 49-58 serpin family E member 1 Homo sapiens 11-16 1703436-7 1991 Binding of PAI-1 to heparin-Sepharose can be efficiently competed with heparin in solution (IC50, 7 microM). Sepharose 28-37 serpin family E member 1 Homo sapiens 11-16 1900197-8 1991 The eCRP formed a precipitin line with histone, protamine, poly(L-lysine) and poly(L-arginine) in agarose gel. Sepharose 98-105 ribonuclease A family member 2C, pseudogene Homo sapiens 4-8 1824821-3 1991 Insulin binding activity was purified from Triton X-100 solubilized membranes in two steps: FPLC on a MonoQ HR5/5 column; and affinity chromatography on insulin-agarose. Sepharose 161-168 insulin Homo sapiens 0-7 1824821-3 1991 Insulin binding activity was purified from Triton X-100 solubilized membranes in two steps: FPLC on a MonoQ HR5/5 column; and affinity chromatography on insulin-agarose. Sepharose 161-168 insulin Homo sapiens 153-160 1989599-1 1991 An intracellular form of phospholipase A2 was purified about 47,500-fold to near homogeneity from bovine platelets 100,000 x g supernatant by sequential use of column chromatographies on Heparin-Sepharose, DEAE-Sephacel, Butyl-Toyopearl, Sephacryl S-300, DEAE-5PW HPLC, TSK G 3000 SW HPLC and Mono Q FPLC. Sepharose 195-204 LOC104974671 Bos taurus 25-41 1702811-7 1991 p150,95 purified under these conditions with CBRp150/4G1-Sepharose could be immunoprecipitated by using antibodies to the alpha- and beta-chains of p150,95 indicating that the structural integrity of the heterodimer was preserved during purification and elution. Sepharose 57-66 chromatin assembly factor 1 subunit A Homo sapiens 0-4 1702811-7 1991 p150,95 purified under these conditions with CBRp150/4G1-Sepharose could be immunoprecipitated by using antibodies to the alpha- and beta-chains of p150,95 indicating that the structural integrity of the heterodimer was preserved during purification and elution. Sepharose 57-66 chromatin assembly factor 1 subunit A Homo sapiens 48-52 1703296-1 1991 The soluble form of guanylyl cyclase-activating-factor (GAF) synthase from rat cerebellum was purified to homogeneity by sequential affinity chromatographic steps on adenosine 2",5"-bisphosphate (2",5"-ADP)-Sepharose and calmodulin-agarose. Sepharose 207-216 fibroblast growth factor 9 Rattus norvegicus 20-54 1703296-1 1991 The soluble form of guanylyl cyclase-activating-factor (GAF) synthase from rat cerebellum was purified to homogeneity by sequential affinity chromatographic steps on adenosine 2",5"-bisphosphate (2",5"-ADP)-Sepharose and calmodulin-agarose. Sepharose 207-216 fibroblast growth factor 9 Rattus norvegicus 56-59 1703296-1 1991 The soluble form of guanylyl cyclase-activating-factor (GAF) synthase from rat cerebellum was purified to homogeneity by sequential affinity chromatographic steps on adenosine 2",5"-bisphosphate (2",5"-ADP)-Sepharose and calmodulin-agarose. Sepharose 232-239 fibroblast growth factor 9 Rattus norvegicus 20-54 1703296-1 1991 The soluble form of guanylyl cyclase-activating-factor (GAF) synthase from rat cerebellum was purified to homogeneity by sequential affinity chromatographic steps on adenosine 2",5"-bisphosphate (2",5"-ADP)-Sepharose and calmodulin-agarose. Sepharose 232-239 fibroblast growth factor 9 Rattus norvegicus 56-59 1824693-9 1991 The asparagine mutant retained its affinity for calmodulin-Sepharose while the aspartate mutant did not adsorb to calmodulin-Sepharose. Sepharose 59-68 calmodulin 1 Homo sapiens 48-58 1846291-6 1991 A MAP2 kinase has been purified from insulin-treated rat 1 HIRc B cells over 6300-fold by chromatography on Q-Sepharose, phenyl-Sepharose, S-Sepharose, phosphocellulose, QAE-Sepharose, UltrogelAcA54, DEAE-cellulose, and a second Q-Sepharose. Sepharose 139-150 microtubule-associated protein 2 Rattus norvegicus 2-6 2036297-1 1991 Chemotaxis of human neutrophils in response to a gradient of the chemotactic peptide, fmet-leu-phe (FMLP), was measured by the under-agarose technique. Sepharose 133-140 formyl peptide receptor 1 Homo sapiens 86-98 1985971-10 1991 The Fab fragments of specific IgG preparations, affinity-purified on a factor VII-agarose column, inhibited the rate of factor X activation in a dose-dependent manner. Sepharose 82-89 FA complementation group B Homo sapiens 4-7 2021239-6 1991 The array of vWf multimers revealed by sodium dodecyl sulfate-agarose gel electrophoresis of canine platelet lysates had a distinct appearance that differed from that of vWf in canine or human plasma and platelets; the intensity of the canine platelet vWf multimer bands was skewed, with relatively greater density in the lower molecular weight region and faint or undetectable multimer bands in the higher molecular weight region. Sepharose 62-69 von Willebrand factor Canis lupus familiaris 13-16 1759731-3 1991 alpha 1-antichymotrypsin purified from the serum of a single healthy donor was separated into three forms by affinity chromatography on a Con A-Sepharose 4B column: a Con A non-reactive form, a Con A weakly reactive form and a Con A reactive form. Sepharose 144-153 serpin family A member 3 Homo sapiens 0-24 1892248-1 1991 New adenosine deaminase variants ADA C and ADA D were found by means of agarose gel electrophoresis in pig erythrocytes. Sepharose 72-79 adenosine deaminase Sus scrofa 4-23 1892248-1 1991 New adenosine deaminase variants ADA C and ADA D were found by means of agarose gel electrophoresis in pig erythrocytes. Sepharose 72-79 adenosine deaminase Sus scrofa 33-36 1892248-1 1991 New adenosine deaminase variants ADA C and ADA D were found by means of agarose gel electrophoresis in pig erythrocytes. Sepharose 72-79 adenosine deaminase Sus scrofa 43-46 2018350-0 1991 Correlation of growth capacity of cells in hard agarose with successful transfection by the activated c-Ha-ras oncogene and in vivo proliferative capacity at metastatic sites. Sepharose 48-55 Harvey rat sarcoma virus oncogene Mus musculus 102-110 2018350-1 1991 The purpose of this study was to determine whether the degree of anchorage-independent growth of rodent or human cells in increasing concentrations of agarose correlated with successful transfection of the cells with an activated c-Ha-ras oncogene and tumorigenicity in nude mice. Sepharose 151-158 Harvey rat sarcoma virus oncogene Mus musculus 230-238 2036473-1 1991 The postnatal development of alcohol dehydrogenase (ADH) isozymes was studied in liver, kidney, stomach and eye tissues of C57BL/6J inbred male mice using agarose isoelectric focusing and histochemical methods. Sepharose 155-162 aldo-keto reductase family 1, member A1 (aldehyde reductase) Mus musculus 29-50 1368072-1 1991 The interaction of several serum albumins with chelated (iminodiacetate, IDA) and immobilized (agarose-IDA) metal ions, Co2+, Ni2+, Cu2+ and Zn2+, was studied. Sepharose 95-102 alpha-L-iduronidase Homo sapiens 103-106 1368084-2 1991 Using conventional NaCl gradient methodology it was found that the order of elution of specific plasma proteins, and the yield of VIII/vWf, varied with the methods used to derivatize the agarose beads. Sepharose 187-194 cytochrome c oxidase subunit 8A Homo sapiens 130-134 1368084-2 1991 Using conventional NaCl gradient methodology it was found that the order of elution of specific plasma proteins, and the yield of VIII/vWf, varied with the methods used to derivatize the agarose beads. Sepharose 187-194 von Willebrand factor Homo sapiens 135-138 1998601-12 1991 The site of cleavage was determined by incubation of AT Vicenza (/Charleville) with thrombin-Sepharose, reduction and S-carboxymethylation and reverse phase FPLC. Sepharose 93-102 coagulation factor II, thrombin Homo sapiens 84-92 2036297-1 1991 Chemotaxis of human neutrophils in response to a gradient of the chemotactic peptide, fmet-leu-phe (FMLP), was measured by the under-agarose technique. Sepharose 133-140 formyl peptide receptor 1 Homo sapiens 100-104 1894195-1 1991 A study by an agarose technique of the chemotactic power of human alpha-thrombin on polymorphonuclear leukocytes reveals that this enzyme has a chemotactic activity. Sepharose 14-21 coagulation factor II, thrombin Homo sapiens 72-80 2065436-11 1991 Cathepsin B had no affinity for concanavalin A-Sepharose due to the absence of glycoprotein content, unlike cathepsin L which showed a strong affinity for concanavalin A-Sepharose. Sepharose 170-179 cathepsin L Homo sapiens 108-119 1721018-2 1991 In the present study we have found that articular proteoglycans from human and baboon separated on agarose flat-bed gels and blotted onto nitrocellulose react with a rabbit antiserum to mouse EGF. Sepharose 99-106 epidermal growth factor Mus musculus 192-195 1756615-11 1991 Immunoabsorption chromatography using an anti-human BAT antibody-Sepharose affinity column showed that both the immunoreactive protein band and BAT activity were removed from the 100,000 g supernatant fraction from human and rat livers. Sepharose 65-74 bile acid-CoA:amino acid N-acyltransferase Homo sapiens 52-55 1756615-11 1991 Immunoabsorption chromatography using an anti-human BAT antibody-Sepharose affinity column showed that both the immunoreactive protein band and BAT activity were removed from the 100,000 g supernatant fraction from human and rat livers. Sepharose 65-74 bile acid-CoA:amino acid N-acyltransferase Homo sapiens 144-147 1838311-2 1991 Annexin V has been purified from Triton X-100 extracts of porcine gastric mucosal membranes by a combination of chromatography on concanavalin A-Sepharose and DEAE-Sepharose, and preparative gel electrophoresis. Sepharose 145-154 annexin A5 Homo sapiens 0-9 1721035-1 1991 Positive affinity chromatography on heparin-Sepharose has proved a most crucial step in the purification of pregnancy-associated plasma protein A (PAPP-A). Sepharose 44-53 pappalysin 1 Homo sapiens 108-145 1721035-1 1991 Positive affinity chromatography on heparin-Sepharose has proved a most crucial step in the purification of pregnancy-associated plasma protein A (PAPP-A). Sepharose 44-53 pappalysin 1 Homo sapiens 147-153 1768437-3 1991 ASM cells release in the medium a VEGF-like endothelial cell mitogen which binds to heparin-sepharose and has an apparent molecular weight of 40-45 kDa as assessed by an HPLC gel filtration column. Sepharose 92-101 vascular endothelial growth factor A Homo sapiens 34-38 1828778-1 1991 Phosphoglucomutase-1 (PGM1) phenotyping of 1,128 Chinese blood donors was performed by thin-layer isoelectric focusing on agarose. Sepharose 122-129 phosphoglucomutase 1 Homo sapiens 0-20 1828778-1 1991 Phosphoglucomutase-1 (PGM1) phenotyping of 1,128 Chinese blood donors was performed by thin-layer isoelectric focusing on agarose. Sepharose 122-129 phosphoglucomutase 1 Homo sapiens 22-26 1830289-1 1991 Phosphoglucomutase 1 (PGM1) phenotyping was performed in 1,128 Chinese blood donors by thin-layer isoelectric focusing on agarose. Sepharose 122-129 phosphoglucomutase 1 Homo sapiens 0-20 1830289-1 1991 Phosphoglucomutase 1 (PGM1) phenotyping was performed in 1,128 Chinese blood donors by thin-layer isoelectric focusing on agarose. Sepharose 122-129 phosphoglucomutase 1 Homo sapiens 22-26 1839153-1 1991 Phosphoglucomutase-1 (PGM1) phenotyping among Taiwanese Chinese was carried out on thin layer agarose gel using isoelectric focusing techniques. Sepharose 94-101 phosphoglucomutase 1 Homo sapiens 0-20 1839153-1 1991 Phosphoglucomutase-1 (PGM1) phenotyping among Taiwanese Chinese was carried out on thin layer agarose gel using isoelectric focusing techniques. Sepharose 94-101 phosphoglucomutase 1 Homo sapiens 22-26 2062560-2 1991 Both the variant and normal antithrombin component were purified by affinity chromatography on heparin Sepharose. Sepharose 103-112 serpin family C member 1 Homo sapiens 28-40 2049182-1 1991 A heparin-binding protein with neurotrophic activity for perinatal rat neurons, termed HBNF, was purified to homogeneity from bovine brain utilizing pH 4.5 extraction, ammonium sulfate precipitation, cation exchange and heparin-Sepharose affinity chromatographies, and reverse phase HPLC. Sepharose 228-237 pleiotrophin Rattus norvegicus 87-91 1786856-2 1991 A new purification procedure was started with salting-out fractionation of serum proteins at 45-75% saturated ammonium sulfate concentration, followed by HE agarose affinity chromatography by which calcium-dependently bound CRP and SAP were purely eluted with EDTA-containing buffer. Sepharose 157-164 amyloid P component, serum Bos taurus 232-235 1815762-5 1991 A mixture of four monoclonal antibodies precipitating C4b-BP both in agarose gel and in solution was used to develop a highly reproducible radial immunodiffusion method for the measurement of C4b-BP in human serum. Sepharose 69-76 complement C4B (Chido blood group) Homo sapiens 54-57 2015950-2 1991 A triglyceride (TG) lipase is present in whole homogenate and tissue extracts of beef myocardium with characteristics of lipoprotein lipase (LPL); i.e., activity is stimulated by serum, inhibited by NaCl and protamine sulfate, the protein binds to heparin-Sepharose, and the enzyme has an alkaline pH optimum. Sepharose 256-265 lipoprotein lipase Bos taurus 121-139 2015950-2 1991 A triglyceride (TG) lipase is present in whole homogenate and tissue extracts of beef myocardium with characteristics of lipoprotein lipase (LPL); i.e., activity is stimulated by serum, inhibited by NaCl and protamine sulfate, the protein binds to heparin-Sepharose, and the enzyme has an alkaline pH optimum. Sepharose 256-265 lipoprotein lipase Bos taurus 141-144 1826678-2 1991 HGF was purified about 400-fold in 10% yield from cirrhotic ascites by ultrafiltration, cation-exchange chromatography on a S-Sepharose column, and affinity chromatography on a heparin-Sepharose CL-6B column. Sepharose 126-135 hepatocyte growth factor Homo sapiens 0-3 1701184-0 1991 A cytochemical staining procedure for succinate dehydrogenase activity in pre-ovulatory mouse oocytes embedded in low gelling temperature agarose. Sepharose 138-145 aminoadipate-semialdehyde synthase Mus musculus 38-61 1845769-4 1991 Alkaline agarose gel analysis of first-strand cDNA synthesis showed an upregulation of POMC transcripts in CRF-treated cells. Sepharose 9-16 pro-opiomelanocortin-alpha Mus musculus 87-91 1721948-2 1991 ApoB obtained by traditional gel filtration in sepharose 4B and high-performance liquid chromatography was used as an antigen. Sepharose 47-56 apolipoprotein B Homo sapiens 0-4 1707136-10 1991 Finally, it was found that Sepharose-linked C1.18 mAb, in the presence of rIL-2 or PMA, did not induce TNF release from 6 NK cell clones. Sepharose 27-36 interleukin 2 Rattus norvegicus 74-79 1850645-2 1991 IL-1 beta plasmid RNA was cut by restriction endonucleases EcoRI and XbaI, and after that it was 1 loaded in 0.75% low-melting-temperature agarose and underwent electrophoresis. Sepharose 139-146 interleukin 1 beta Mus musculus 0-9 1963605-2 1990 In order to determine whether this effect is mediated by a direct interaction with calmodulin, we measured the effects of radiolabelled triflucarbine in a direct ligand binding assay, using agarose-immobilized calmodulin. Sepharose 190-197 calmodulin 1 Homo sapiens 210-220 1963605-3 1990 [3H]Tiflucarbine associated with low micromolar affinity with an apparently homogeneous class of recognition sites on calmodulin-agarose. Sepharose 129-136 calmodulin 1 Homo sapiens 118-128 2265247-3 1990 Following immunoisolation and analysis by polyacrylamide or agarose gel electrophoresis, metabolically labeled vWF was found to have incorporated [35S]-sulfate into all secreted multimer species. Sepharose 60-67 von Willebrand factor Homo sapiens 111-114 2176546-12 1990 Partial purification of this protein utilizing salt precipitation, hydrophobic-interaction chromatography and an affinity step with Affi-Gel Blue Sepharose resulted in a 100-fold purification and a 29% overall yield of NDP-kinase activity. Sepharose 146-155 norrin cystine knot growth factor NDP S homeolog Xenopus laevis 219-222 1965307-7 1990 Calmodulin-binding proteins analyzed by [125I]iodocalmodulin overlay and calmodulin-Sepharose affinity chromatography were also identified in the same cytoskeletons including alpha-spectrin, the Ca2+ calmodulin-dependent phosphatase calcineurin and three that were tentatively identified as the two subunits of the kinase itself and myosin light chain kinase. Sepharose 84-93 calmodulin 2 Mus musculus 0-10 1965307-7 1990 Calmodulin-binding proteins analyzed by [125I]iodocalmodulin overlay and calmodulin-Sepharose affinity chromatography were also identified in the same cytoskeletons including alpha-spectrin, the Ca2+ calmodulin-dependent phosphatase calcineurin and three that were tentatively identified as the two subunits of the kinase itself and myosin light chain kinase. Sepharose 84-93 calmodulin 2 Mus musculus 73-83 2126656-2 1990 We describe the preparation of monospecific antisera against a thyroxine-binding globulin partially purified from immature rat sera by affinity chromatography on thyroxine-Sepharose. Sepharose 172-181 serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7 Mus musculus 63-89 2174432-2 1990 Aldose reductase was purified from human skeletal and heart muscle by a rapid and efficient scheme involving Red Sepharose chromatography, chromatofocusing on Pharmacia PBE 94, and hydroxylapatite high pressure liquid chromatography. Sepharose 113-122 aldo-keto reductase family 1 member B Homo sapiens 0-16 2286014-3 1990 The antibody IgG, purified by the use of Mn SOD-coupled Sepharose, showed a single band on the immunoblotting test with a crude liver extract. Sepharose 56-65 superoxide dismutase 2 Homo sapiens 41-47 2279633-4 1990 Meanwhile, WGA-containing agarose gel electrophoresis revealed that normal controls had four ChE isozymes (named bands I to IV from the anodic side to the cathodic). Sepharose 26-33 butyrylcholinesterase Homo sapiens 93-96 2089037-5 1990 When this soluble fraction was subjected to heparin-Sepharose column chromatography, phospholipase A2 activity was detected in both heparin-binding and heparin-non-binding fractions. Sepharose 52-61 phospholipase A2 Oryctolagus cuniculus 85-101 2126013-4 1990 Internalization of 125I-biotin-S-S-transferrin (125I-BSST) was quantitated by adsorption to avidin-Sepharose after treatment of cells with GSH. Sepharose 99-108 transferrin Homo sapiens 35-46 2081965-7 1990 The apparent beta-endorphin level of plasma from multiple myeloma patients was markedly decreased after affinity chromatography of the serum on protein A-Sepharose. Sepharose 154-163 proopiomelanocortin Homo sapiens 13-27 1829870-2 1991 For the routine subtyping of PGM1 isozymes by means of agarose gel thin-layer electrophoresis, a tris-maleic acid-buffer and an acetic acid-buffer, several agaroses with electroendosmosis up to -0, 16, and several papers are tested. Sepharose 55-62 phosphoglucomutase 1 Homo sapiens 29-33 1829870-2 1991 For the routine subtyping of PGM1 isozymes by means of agarose gel thin-layer electrophoresis, a tris-maleic acid-buffer and an acetic acid-buffer, several agaroses with electroendosmosis up to -0, 16, and several papers are tested. Sepharose 156-164 phosphoglucomutase 1 Homo sapiens 29-33 2147551-8 1990 Measurements of staining intensity of CGRP-coupled agarose beads indicated that a 12% change in staining intensity corresponded to a 15 to 20% change in the concentration of CGRP or CGRP-like immunoreactive material. Sepharose 51-58 calcitonin-related polypeptide alpha Rattus norvegicus 38-42 2147551-8 1990 Measurements of staining intensity of CGRP-coupled agarose beads indicated that a 12% change in staining intensity corresponded to a 15 to 20% change in the concentration of CGRP or CGRP-like immunoreactive material. Sepharose 51-58 calcitonin-related polypeptide alpha Rattus norvegicus 174-178 2147551-8 1990 Measurements of staining intensity of CGRP-coupled agarose beads indicated that a 12% change in staining intensity corresponded to a 15 to 20% change in the concentration of CGRP or CGRP-like immunoreactive material. Sepharose 51-58 calcitonin-related polypeptide alpha Rattus norvegicus 174-178 1705771-2 1990 Human placental glutathione S-transferase was purified to apparent homogeneity by direct application of the crude homogenate into glutathione linked sepharose affinity chromatography. Sepharose 149-158 glutathione S-transferase kappa 1 Homo sapiens 16-41 1708670-7 1990 In the immunopurification of placental extract on anti-PAI-2 Sepharose, the eluate showed the expected reaction with Mab" PAI-2, and it also cross-reacted with anti-vitronectin serum. Sepharose 61-70 serpin family B member 2 Homo sapiens 55-60 1708670-7 1990 In the immunopurification of placental extract on anti-PAI-2 Sepharose, the eluate showed the expected reaction with Mab" PAI-2, and it also cross-reacted with anti-vitronectin serum. Sepharose 61-70 serpin family B member 2 Homo sapiens 122-127 1708670-7 1990 In the immunopurification of placental extract on anti-PAI-2 Sepharose, the eluate showed the expected reaction with Mab" PAI-2, and it also cross-reacted with anti-vitronectin serum. Sepharose 61-70 vitronectin Homo sapiens 165-176 1708670-8 1990 In order to complement these results, anti-vitronectin Sepharose was used for immunopurification of placenta extract. Sepharose 55-64 vitronectin Homo sapiens 43-54 2147196-7 1990 The binding of the OVA-binding GIF in the EGTA extracts to OVA-Sepharose was inhibited by a synthetic peptide, which corresponds to amino acid residues 307-317 in the OVA molecule and represents the epitope recognized by TCR on the cells. Sepharose 63-72 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 31-34 2246516-2 1990 After 45 min incubation with IgG-coated Sepharose beads the concentrations of cell-associated PAF recovered from normal density eosinophils were significantly greater than from low-density eosinophils or neutrophils. Sepharose 40-49 PCNA clamp associated factor Homo sapiens 94-97 1964699-3 1990 Purified human neutrophil myeloperoxidase (MPO) bound tightly to HA-Sepharose and we suggest that this is due to a strong ionic interaction between HA and MPO. Sepharose 68-77 myeloperoxidase Homo sapiens 26-41 2074655-6 1990 MIF activity was determined in supernatants of cultures of isolated glomeruli by the agarose microdroplet method. Sepharose 85-92 macrophage migration inhibitory factor Oryctolagus cuniculus 0-3 2173782-7 1990 Elution of HSV-1-infected cell proteins from single-stranded DNA agarose columns by using a linear KCl gradient demonstrated that the UL37 protein elutes from this matrix at a salt concentration similar to that observed for ICP8, the major HSV-1 DNA-binding protein. Sepharose 65-72 tegument protein UL37 Human alphaherpesvirus 1 134-138 2082734-1 1990 The abundant soluble protein of bovine cornea (CSP) was partially purified by chromatographies on Sephacryl S-200, DEAE cellulose and agarose to which mouse anti-CSP monoclonal antibody produced by the hybridoma technique was conjugated. Sepharose 134-141 dnaJ homolog subfamily C member 5 Bos taurus 47-50 1964699-3 1990 Purified human neutrophil myeloperoxidase (MPO) bound tightly to HA-Sepharose and we suggest that this is due to a strong ionic interaction between HA and MPO. Sepharose 68-77 myeloperoxidase Homo sapiens 43-46 1964699-3 1990 Purified human neutrophil myeloperoxidase (MPO) bound tightly to HA-Sepharose and we suggest that this is due to a strong ionic interaction between HA and MPO. Sepharose 68-77 myeloperoxidase Homo sapiens 155-158 2151255-1 1990 Liposome (ApoA-1: PC) was prepared by the deoxycholic acid dialysis method and identified by agarose gel electrophoresis, two dimensional thin-layer chromatography and electron microscopy. Sepharose 93-100 apolipoprotein A1 Homo sapiens 10-16 2261984-1 1990 Soluble CD4-immunoglobulin chimeric proteins were covalently attached to CNBr-activated Sepharose. Sepharose 88-97 CD4 molecule Homo sapiens 8-11 2127808-4 1990 At the time of demonstrating decreased HRGP values, expression of HRGP fragment was studied using heparin-Sepharose beads. Sepharose 106-115 histidine rich glycoprotein Homo sapiens 66-70 2148644-0 1990 Phagocytosis of agarose beads by receptors for C3b (CR1) and iC3b (CR3) on alveolar macrophages from patients with sarcoidosis. Sepharose 16-23 complement C3 Homo sapiens 47-50 2148644-0 1990 Phagocytosis of agarose beads by receptors for C3b (CR1) and iC3b (CR3) on alveolar macrophages from patients with sarcoidosis. Sepharose 16-23 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 52-55 2148644-0 1990 Phagocytosis of agarose beads by receptors for C3b (CR1) and iC3b (CR3) on alveolar macrophages from patients with sarcoidosis. Sepharose 16-23 teratocarcinoma-derived growth factor 1 pseudogene 3 Homo sapiens 67-70 2148644-5 1990 AM from seven patients with active sarcoidosis and seven healthy controls were cultured under serum-free conditions for 2, 12, 24, and 48 h. We found a significantly increased CR1 and CR3 receptor-mediated phagocytosis of native agarose beads by AM from the seven patients. Sepharose 229-236 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 176-179 2148644-5 1990 AM from seven patients with active sarcoidosis and seven healthy controls were cultured under serum-free conditions for 2, 12, 24, and 48 h. We found a significantly increased CR1 and CR3 receptor-mediated phagocytosis of native agarose beads by AM from the seven patients. Sepharose 229-236 teratocarcinoma-derived growth factor 1 pseudogene 3 Homo sapiens 184-187 2211700-9 1990 Subsequently, hexasaccharides with the highest affinity for HCII were isolated by overloading the HCII-Sepharose column. Sepharose 103-112 serpin family D member 1 Homo sapiens 60-64 2211700-9 1990 Subsequently, hexasaccharides with the highest affinity for HCII were isolated by overloading the HCII-Sepharose column. Sepharose 103-112 serpin family D member 1 Homo sapiens 98-102 2211713-6 1990 Solubilized receptor is specifically immunoprecipitated by sheep polyclonal antibodies against purified skeletal muscle ryanodine receptor coupled to protein A-Sepharose. Sepharose 160-169 ryanodine receptor 1 Oryctolagus cuniculus 104-138 2211716-2 1990 This results in an increase in the amount of GPIIb/IIIa adsorbed by GRGDSPK- and L10-Sepharose by 12-20-fold. Sepharose 85-94 integrin subunit alpha 2b Homo sapiens 45-50 2226463-7 1990 The similarity was higher than 60% on peptide mapping with trypsin digestion, the 105-kDa protein cross-reacted with anti-HSP90 antibody, both were bound similarly to heparin-Sepharose gel and both are located in the cytosol fraction. Sepharose 175-184 heat shock protein 90 alpha family class A member 1 Rattus norvegicus 122-127 2171680-7 1990 Recombinant SP-A was purified from the serum-free media of large scale cultures of transfected, amplified CHO cells by affinity chromatography on mannose-Sepharose. Sepharose 154-163 surfactant protein A1 Rattus norvegicus 12-16 2147709-8 1990 Pre-absorption of anti-lipocortin-1 antiserum with lipocortin-1-coupled Sepharose-4B immunoreactivity resulted in loss of immunoreactivity in both lobes. Sepharose 72-81 annexin A1 Homo sapiens 23-35 2147709-8 1990 Pre-absorption of anti-lipocortin-1 antiserum with lipocortin-1-coupled Sepharose-4B immunoreactivity resulted in loss of immunoreactivity in both lobes. Sepharose 72-81 annexin A1 Homo sapiens 51-63 2278835-2 1990 Ammonium sulphate fractionation followed by immunoaffinity chromatography on a three column system using Protein A-Sepharose coupled D5, produced purified p29. Sepharose 115-124 SYF2 pre-mRNA splicing factor Homo sapiens 155-158 2147182-5 1990 Approximately 2 g of the placental membrane extract was subjected to a biotinylated GM-CSF-fixed streptavidin-agarose column, resulting in a single major band at 70 kDa on a silver-stained sodium dodecyl sulfate gel. Sepharose 110-117 colony stimulating factor 2 Homo sapiens 84-90 2264821-5 1990 The binding of the receptor to IgA-Sepharose was inhibited in a dose-dependent manner by human serum IgA1 or IgA2 or secretory IgA1 or IgA2, but not by IgG or IgM. Sepharose 35-44 immunoglobulin heavy constant alpha 1 Homo sapiens 101-105 2264821-5 1990 The binding of the receptor to IgA-Sepharose was inhibited in a dose-dependent manner by human serum IgA1 or IgA2 or secretory IgA1 or IgA2, but not by IgG or IgM. Sepharose 35-44 immunoglobulin heavy constant alpha 1 Homo sapiens 127-131 2282978-2 1990 The heat shock protein (hsp-90) bound to receptor was precipitated with monoclonal antibodies H222 or JS 34/32, coupled to protein A-Sepharose and purified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions. Sepharose 133-142 heat shock protein 90 alpha family class A member 1 Homo sapiens 24-30 2172248-1 1990 Three isozymes of diacylglycerol kinase (DGK), DGK-I, DGK-II, and DGK-III, were purified from the cytosol of human platelets by successive chromatography on DEAE-cellulose, Ultrogel AcA34, heparin-Sepharose, ATP-agarose, Mono Q, phenyl-Superose, HCA-hydroxyapatite, Wakopak G40, and TSK-3000SW columns. Sepharose 197-206 diacylglycerol kinase beta Homo sapiens 18-39 2172248-1 1990 Three isozymes of diacylglycerol kinase (DGK), DGK-I, DGK-II, and DGK-III, were purified from the cytosol of human platelets by successive chromatography on DEAE-cellulose, Ultrogel AcA34, heparin-Sepharose, ATP-agarose, Mono Q, phenyl-Superose, HCA-hydroxyapatite, Wakopak G40, and TSK-3000SW columns. Sepharose 197-206 diacylglycerol kinase beta Homo sapiens 41-44 2229039-1 1990 A neurite-promoting factor (p18) was isolated from bovine brain using ammonium sulfate fractionation, sulfated Sephadex G-50 chromatography, heparin-Sepharose gel chromatography, and reverse phase high performance liquid chromatography. Sepharose 149-158 pleiotrophin Bos taurus 28-31 2230149-1 1990 Native human haptoglobin isolated from normal human plasma by affinity chromatography on chicken hemoglobin -Sepharose was used as standard antigen. Sepharose 109-118 haptoglobin Homo sapiens 13-24 2241151-9 1990 The brain HSP90 was separable from glucocorticoid receptor by heparin-agarose and DNA-cellulose columns. Sepharose 70-77 heat shock protein 90 alpha family class A member 1 Homo sapiens 10-15 2125828-3 1990 LDL incubated with PLA2 showed increased monoacylphospholipid content, decreased diacylphospholipid content, and increased anodic migration on agarose gel electrophoresis. Sepharose 143-150 phospholipase A2 group IB Homo sapiens 19-23 2128190-0 1990 [Specific binding of iodothyronines with apolipoprotein A-1 in high density lipoprotein particle isolated from human serum by affinity chromatography on thyroxine-sepharose]. Sepharose 163-172 apolipoprotein A1 Homo sapiens 41-59 2128191-2 1990 A homogeneous aldose reductase was isolated from bovine eye lens tissue by using affinity chromatography on blue agarose. Sepharose 113-120 aldose reductase Bos taurus 14-30 2268430-3 1990 Fractions from Q Sepharose chromatography, eluted with a linear gradient 0-1.0 M NaCl and subsequently acetylated, proved to be the most effective method for obtaining deoxyribonuclease-free bovine serum albumin. Sepharose 17-26 albumin Homo sapiens 198-211 2146997-8 1990 In contrast to agar culture, CD8 colonies can be derived from quiescent CD8 lymphocytes in agarose. Sepharose 91-98 CD8a molecule Homo sapiens 29-32 2146997-8 1990 In contrast to agar culture, CD8 colonies can be derived from quiescent CD8 lymphocytes in agarose. Sepharose 91-98 CD8a molecule Homo sapiens 72-75 2146997-10 1990 CD3-TcR modulation with anti-CD3 monoclonal antibody prior to culturing in agarose inhibits the colony formation. Sepharose 75-82 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 4-7 2146276-12 1990 Scatter factor was purified to homogeneity from conditioned medium of human fibroblasts by heparin-Sepharose chromatography, followed by cation exchange chromatography, gel filtration, or preparative SDS gel electrophoresis. Sepharose 99-108 hepatocyte growth factor Canis lupus familiaris 0-14 2229188-2 1990 The protein was isolated by affinity chromatography of detergent extracts on wheat germ lectin-Agarose followed by chromatography of the WGA binding fraction on fibronectin-Sepharose. Sepharose 173-182 fibronectin 1 Rattus norvegicus 161-172 2246329-7 1990 Nuclear extracts prepared from transfected cells were found to contain bFGF as determined using heparin-sepharose affinity chromatography, followed by Western blot analysis of fractions, which stimulated the proliferation BHK-21 cells. Sepharose 104-113 fibroblast growth factor 2 Homo sapiens 71-75 2075729-1 1990 Acid-stable trypsin inhibitor was isolated from urine of healthy persons; its homogeneous preparation was obtained using absorption on chitosan, affinity chromatography on chymotrypsin-Sepharose 4B and gel filtration of Sephadex G-100. Sepharose 185-194 cysteine rich secretory protein LCCL domain containing 2 Homo sapiens 12-29 2084949-0 1990 Multimeric analysis of von Willebrand factor by molecular sieving electrophoresis in sodium dodecyl sulphate agarose gel. Sepharose 109-116 von Willebrand factor Homo sapiens 23-44 2084949-1 1990 We report the development and optimisation of an agarose gel electrophoretic method for the separation and detection of von Willebrand Factor (vWF) multimers. Sepharose 49-56 von Willebrand factor Homo sapiens 120-141 2084949-1 1990 We report the development and optimisation of an agarose gel electrophoretic method for the separation and detection of von Willebrand Factor (vWF) multimers. Sepharose 49-56 von Willebrand factor Homo sapiens 143-146 1698790-4 1990 In control studies anti-rhodopsin monoclonal antibody (Rho 1D4)-Sepharose beads removed residual rhodopsin, but not the 63/240-kDa complex or channel activity. Sepharose 64-73 rhodopsin Bos taurus 24-33 1698790-4 1990 In control studies anti-rhodopsin monoclonal antibody (Rho 1D4)-Sepharose beads removed residual rhodopsin, but not the 63/240-kDa complex or channel activity. Sepharose 64-73 rhodopsin Bos taurus 97-106 2170522-2 1990 Coincubation of neutrophils with TNF inhibited the chemoattractant-directed migration of neutrophils under agarose and enhanced their migration in the multiwell chemotaxis chamber. Sepharose 107-114 tumor necrosis factor Mus musculus 33-36 2171678-6 1990 The use of a novel affinity column, a V1-vasopressin antagonist-agarose, resulted in a near-homogeneous preparation of a protein which exhibited an Mr = 58,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 64-71 arginine vasopressin Rattus norvegicus 41-52 2173560-1 1990 Human insulin-like growth factor I, IGF-I, was produced in Escherichia coli fused to a synthetic IgG-binding peptide The fusion protein is secreted into the medium during fermentation and was initially purified on an IgG-Sepharose column. Sepharose 221-230 insulin like growth factor 1 Homo sapiens 6-34 2173560-1 1990 Human insulin-like growth factor I, IGF-I, was produced in Escherichia coli fused to a synthetic IgG-binding peptide The fusion protein is secreted into the medium during fermentation and was initially purified on an IgG-Sepharose column. Sepharose 221-230 insulin like growth factor 1 Homo sapiens 36-41 2207328-6 1990 Heparin-Sepharose chromatography resulted in the elution of cell-free derived AT-III as a broad peak between 0.2 and 0.7 mol/L NaCl. Sepharose 8-17 serpin family C member 1 Homo sapiens 78-84 2170375-1 1990 The bombesin/gastrin-releasing peptide (GRP) receptor was solubilized from Swiss mouse 3T3 cell membranes in an active form and was purified about 90,000-fold to near homogeneity by a combination of wheat germ agglutinin-agarose and ligand affinity chromatography. Sepharose 221-228 gastrin releasing peptide Mus musculus 40-43 2271625-4 1990 After activation of the crude prochymosin, the enzyme was purified by affinity chromatography on Sepharose with V-dL-P-F-F-V-dL as ligand. Sepharose 97-106 chymosin Bos taurus 30-41 2211593-6 1990 Transfected cells expressed 1-2 micrograms/ml of recombinant EPI (rEPI) which was purified to homogeneity by heparin-Sepharose chromatography, ion-exchange chromatography, and reverse phase high performance liquid chromatography. Sepharose 117-126 tissue factor pathway inhibitor Homo sapiens 61-64 2212692-3 1990 The amount of C3b immobilized by each support varied from 81% for Actigel A aldehyde activated agarose to only 19% for Reacti-Gel GF-2000. Sepharose 95-102 endogenous retrovirus group K member 3 Homo sapiens 14-17 2271595-6 1990 A C5a receptor affinity column was constructed by saturating monoclonal antibody bound to agarose with C5a-N19. Sepharose 90-97 complement C5a receptor 1 Homo sapiens 2-14 2271595-6 1990 A C5a receptor affinity column was constructed by saturating monoclonal antibody bound to agarose with C5a-N19. Sepharose 90-97 complement C5a receptor 1 Homo sapiens 2-5 2221078-4 1990 Pig gastric mucin, partially purified by Sepharose 4B gel chromatography, was found to contain large amounts of free fatty acids and cholesterol as well as lesser amounts of sphingomyelin and phospholipids. Sepharose 41-50 LOC100188911 Sus scrofa 4-17 2221913-3 1990 Two thioltransferase activity peaks were resolved by CM-Sepharose chromatography. Sepharose 56-65 glutaredoxin Homo sapiens 4-20 2221914-1 1990 The rat mitochondrial single strand DNA binding protein (SSB) P16 was purified to apparent homogeneity by elution from single strand DNA agarose with ethidium bromide. Sepharose 137-144 small RNA binding exonuclease protection factor La Rattus norvegicus 22-55 2221914-1 1990 The rat mitochondrial single strand DNA binding protein (SSB) P16 was purified to apparent homogeneity by elution from single strand DNA agarose with ethidium bromide. Sepharose 137-144 small RNA binding exonuclease protection factor La Rattus norvegicus 57-60 2221914-1 1990 The rat mitochondrial single strand DNA binding protein (SSB) P16 was purified to apparent homogeneity by elution from single strand DNA agarose with ethidium bromide. Sepharose 137-144 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 62-65 2222404-3 1990 The lipase bound to heparin-agarose and co-eluted with 125I-labelled bovine lipoprotein lipase in a salt gradient. Sepharose 28-35 lipoprotein lipase Bos taurus 76-94 2123414-1 1990 A globin-agarose affinity chromatography technique was used to purify swine haptoglobin. Sepharose 9-16 haptoglobin Sus scrofa 76-87 2208661-0 1990 Improved method for identifying alpha 1-antitrypsin Pi M subtypes by isoelectric focusing in agarose. Sepharose 93-100 serpin family A member 1 Homo sapiens 32-51 2208661-1 1990 We describe an improved method for the classification of alpha 1-antitrypsin variants by isoelectric focusing in agarose. Sepharose 113-120 serpin family A member 1 Homo sapiens 57-76 2170154-5 1990 Both the alpha 1 beta 1-integrin and the 145,000/beta 1-integrin heterodimers bound specifically to Sepharose beads derivatized with the collagen fragment alpha 1(I) CB3, which lacks RGD sequences. Sepharose 100-109 integrin subunit beta 1 Homo sapiens 17-32 2171432-6 1990 Membrane proteins were labeled with 125I and passed through an affinity column in which CP was covalently linked to Sepharose 4B. Sepharose 116-125 ceruloplasmin Rattus norvegicus 88-90 2209543-2 1990 A protein factor that binds to pOTC but not to mature OTC and was named presequence binding factor or PBF, was purified 91,000-fold from the lysate by affinity chromatography using pOTC-bound Sepharose, DEAE-5PW HPLC and sucrose gradient centrifugation. Sepharose 192-201 PTTG1 interacting protein Rattus norvegicus 72-98 2209543-2 1990 A protein factor that binds to pOTC but not to mature OTC and was named presequence binding factor or PBF, was purified 91,000-fold from the lysate by affinity chromatography using pOTC-bound Sepharose, DEAE-5PW HPLC and sucrose gradient centrifugation. Sepharose 192-201 PTTG1 interacting protein Rattus norvegicus 102-105 2242758-3 1990 The group B receptor was released in an almost pure form from bacteria incubated at elevated pH, and could be isolated by IgA-Sepharose affinity chromatography. Sepharose 126-135 CD79a molecule Homo sapiens 122-125 1701301-5 1990 A fraction of the patient"s anti-Factor VIII auto-antibodies was specifically retained on affinity columns of Sepharose-bound MoAb 20F2; anti-Factor VIII activity of antibodies in this fraction was totally inhibited by MoAb 20F2, indicating an idiotopic homogeneity of retained anti-Factor VIII autoantibodies. Sepharose 110-119 coagulation factor VIII Mus musculus 33-44 1701301-5 1990 A fraction of the patient"s anti-Factor VIII auto-antibodies was specifically retained on affinity columns of Sepharose-bound MoAb 20F2; anti-Factor VIII activity of antibodies in this fraction was totally inhibited by MoAb 20F2, indicating an idiotopic homogeneity of retained anti-Factor VIII autoantibodies. Sepharose 110-119 coagulation factor VIII Mus musculus 142-153 1701301-5 1990 A fraction of the patient"s anti-Factor VIII auto-antibodies was specifically retained on affinity columns of Sepharose-bound MoAb 20F2; anti-Factor VIII activity of antibodies in this fraction was totally inhibited by MoAb 20F2, indicating an idiotopic homogeneity of retained anti-Factor VIII autoantibodies. Sepharose 110-119 coagulation factor VIII Mus musculus 142-153 2170425-2 1990 Iodinated 125I-bFGF diffuses further in agarose, fibrin, and on a monolayer of bovine aortic endothelial (BAE) cells in the presence of heparin than in its absence. Sepharose 40-47 fibroblast growth factor 2 Bos taurus 15-19 2170154-5 1990 Both the alpha 1 beta 1-integrin and the 145,000/beta 1-integrin heterodimers bound specifically to Sepharose beads derivatized with the collagen fragment alpha 1(I) CB3, which lacks RGD sequences. Sepharose 100-109 integrin subunit beta 1 Homo sapiens 49-64 2398274-7 1990 We further demonstrated that the inhibitory IgG which was bound to protein A Sepharose could bind a significant amount of 125I-IL-1 alpha, whereas only a negligible binding of the radiolabeled ligand was detected when IgG without the inhibitory activity was used as control. Sepharose 77-86 interleukin 1 alpha Homo sapiens 127-137 2223064-0 1990 Rapid alkaline transfer of low molecular weight DNA from NuSieve GTG agarose gels. Sepharose 69-76 gamma-glutamyltransferase 1 Homo sapiens 65-68 2292580-0 1990 Isoelectrical focusing of connectin by agarose gel electrophoresis. Sepharose 39-46 titin Homo sapiens 26-35 2292580-1 1990 Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of approximately 3,000 kDa. Sepharose 47-54 titin Homo sapiens 182-191 2292586-3 1990 Affinity chromatography of the sulfated products on antithrombin III-Sepharose gel indicated that the polysaccharide acquired some affinity for the protein following the sulfation, as shown by the increase in the proportion of the high-affinity heparin fraction (%) from 1.1 to 6.7. Sepharose 69-78 serpin family C member 1 Homo sapiens 52-68 1697852-2 1990 Alpha 1-Microglobulin (alpha 1-m), or protein HC, a low molecular weight plasma protein with immunoregulatory properties, was isolated from rat serum by affinity chromatography using Sepharose-coupled monoclonal anti-alpha 1-m antibodies. Sepharose 183-192 pregnancy-zone protein Rattus norvegicus 23-32 2168880-7 1990 The LACIs were purified from these media by immunoaffinity chromatography on an anti-LACI-lg-Sepharose 4B column and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 93-105 tissue factor pathway inhibitor Homo sapiens 4-8 2168890-1 1990 Acidic fibroblast growth factor (aFGF) receptor was purified from plasma membranes of bovine liver using Triton X-100 extraction, wheat germ lectin-Sepharose 4B gel affinity chromatography, and DEAE-cellulose anion-exchange chromatography. Sepharose 148-157 fibroblast growth factor 1 Bos taurus 33-37 2169348-3 1990 Through several purification steps, vimentin kinase activity copurifies with histone H1 kinase and both activities bind to p13suc1-Sepharose. Sepharose 131-140 vimentin Mesocricetus auratus 36-44 2398061-4 1990 Using two steps of affinity chromatography, anti-DDDED-Sepharose and nucleoplasmin-Sepharose, we obtained a protein of 69 kDa (p69) from the nuclear pore fraction that showed these characters. Sepharose 55-64 islet cell autoantigen 1 Homo sapiens 127-130 2398061-4 1990 Using two steps of affinity chromatography, anti-DDDED-Sepharose and nucleoplasmin-Sepharose, we obtained a protein of 69 kDa (p69) from the nuclear pore fraction that showed these characters. Sepharose 83-92 islet cell autoantigen 1 Homo sapiens 127-130 2168346-3 1990 Basic FGF (bFGF) was identified in SW-13 lysates by both its chromatographic behavior on heparin-Sepharose and its reactivity with an antibody specific for bFGF. Sepharose 97-106 fibroblast growth factor 2 Homo sapiens 0-9 2168346-3 1990 Basic FGF (bFGF) was identified in SW-13 lysates by both its chromatographic behavior on heparin-Sepharose and its reactivity with an antibody specific for bFGF. Sepharose 97-106 fibroblast growth factor 2 Homo sapiens 11-15 2264022-1 1990 Chemotactic technique in agarose gel has exposed the attractive properties of human alpha thrombin with respect to human polymorphonuclear leukocytes. Sepharose 25-32 coagulation factor II, thrombin Homo sapiens 90-98 2123288-4 1990 It was shown that both tubulin and calmodulin bind to tau peptide-Sepharose affinity column. Sepharose 66-75 calmodulin 1 Homo sapiens 35-45 2223064-3 1990 Now we report that this rapid alkaline method can be used for the transfer of low molecular weight DNA fragments (10 to 1000 base pairs) from NuSieve GTG agarose gels to nylon membrane. Sepharose 154-161 gamma-glutamyltransferase 1 Homo sapiens 150-153 2253091-1 1990 Human mast cell tryptase was purified from lung tissue by high salt extraction, ammonium sulphate precipitation, octyl Sepharose and heparin-agarose chromatography. Sepharose 141-148 tryptase delta 1 Homo sapiens 6-24 1698717-2 1990 Using a monoclonal anti-SP antibody (termed MASP-1) coupled to Sepharose, it was possible to immunoaffinity purify from culture supernates a peptide that was antigenically related to SP. Sepharose 63-72 tachykinin 1 Mus musculus 24-26 2227796-1 1990 Healthy human serum reacted with C-reactive protein of human (hCRP) or rat (rCRP) immobilized on Sepharose 4B in the presence of Ca2+. Sepharose 97-109 C-reactive protein Homo sapiens 33-51 2277038-1 1990 A rat liver glutathione S-transferase isozyme (GST 3-3) has been purified to apparent electrophoretic homogeneity by procedures including Sephadex G-100, S-hexylglutathione-linked Sepharose, and CM-cellulose column chromatographies. Sepharose 180-189 glutathione S-transferase pi 1 Rattus norvegicus 12-54 2251783-1 1990 Heating of cow milk beta-lactoglobulin at 96 degrees, pH 8.0 led to the protein aggregation because of intermolecular disulfide exchange as shown by agarose gel immunoelectrophoresis, where additional precipitation strips were detected. Sepharose 149-156 beta-lactoglobulin Bos taurus 20-38 1697468-1 1990 A Mn2(+)-dependent serine/threonine protein kinase from rat liver membranes copurifies with the insulin receptor (IR) on wheat germ agglutinin (WGA)-sepharose. Sepharose 149-158 insulin receptor Rattus norvegicus 96-112 1697468-1 1990 A Mn2(+)-dependent serine/threonine protein kinase from rat liver membranes copurifies with the insulin receptor (IR) on wheat germ agglutinin (WGA)-sepharose. Sepharose 149-158 insulin receptor Rattus norvegicus 114-116 2170769-1 1990 Human placental 17 beta-hydroxysteroid dehydrogenase (17-HSD) was purified to apparent homogeneity using ammonium sulfate precipitation and chromatography on Red-Agarose and DEAE-Sepharose columns. Sepharose 162-169 hydroxysteroid 17-beta dehydrogenase 1 Homo sapiens 6-52 2170769-1 1990 Human placental 17 beta-hydroxysteroid dehydrogenase (17-HSD) was purified to apparent homogeneity using ammonium sulfate precipitation and chromatography on Red-Agarose and DEAE-Sepharose columns. Sepharose 162-169 hydroxysteroid 17-beta dehydrogenase 13 Homo sapiens 54-60 2387419-3 1990 The resulting enzyme (HB-SOD) bound to a heparin-Sepharose column and cultured endothelial cells; binding was inhibited either by high NaCl concentrations or heparin. Sepharose 49-58 superoxide dismutase 1 Homo sapiens 25-28 2393668-1 1990 A NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-OH-PGDH) from porcine kidney was purified to homogeneity by acid precipitation, blue agarose affinity chromatography, hydroxyapatite-ultrogel adsorption chromatography, DEAE-Sephadex ion-exchange chromatography and NAD(+)-agarose affinity chromatography. Sepharose 146-153 15-hydroxyprostaglandin dehydrogenase Homo sapiens 2-56 2393668-1 1990 A NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (15-OH-PGDH) from porcine kidney was purified to homogeneity by acid precipitation, blue agarose affinity chromatography, hydroxyapatite-ultrogel adsorption chromatography, DEAE-Sephadex ion-exchange chromatography and NAD(+)-agarose affinity chromatography. Sepharose 146-153 15-hydroxyprostaglandin dehydrogenase Homo sapiens 64-68 2177588-0 1990 Photochemical derivatization of agarose: synthesis of an affinity agarose using a photoaffinity ligand specific for the peripheral-type benzodiazepine receptor. Sepharose 32-39 translocator protein Bos taurus 120-159 2177588-0 1990 Photochemical derivatization of agarose: synthesis of an affinity agarose using a photoaffinity ligand specific for the peripheral-type benzodiazepine receptor. Sepharose 66-73 translocator protein Bos taurus 120-159 2177588-1 1990 PK 14105, a photoaffinity ligand specific for the peripheral-type benzodiazepine receptor (PBZR), was photochemically coupled to omega-aminobutyl agarose (ABAg) to yield PK 14105 agarose (PKAg). Sepharose 146-153 translocator protein Bos taurus 50-89 2177588-1 1990 PK 14105, a photoaffinity ligand specific for the peripheral-type benzodiazepine receptor (PBZR), was photochemically coupled to omega-aminobutyl agarose (ABAg) to yield PK 14105 agarose (PKAg). Sepharose 146-153 translocator protein Bos taurus 91-95 2383599-8 1990 In the presence of Ca2+, chicken gizzard tropomyosin bound to a calmodulin-Sepharose-4B column and was eluted with a salt concentration of 140 mM. Sepharose 75-84 calmodulin 2 Gallus gallus 64-74 1978461-9 1990 P-GP-MRK 16-protein A-Sepharose complex derived from human adrenal possessed marked ATPase activity. Sepharose 22-31 ATP binding cassette subfamily B member 1 Homo sapiens 0-4 1978461-9 1990 P-GP-MRK 16-protein A-Sepharose complex derived from human adrenal possessed marked ATPase activity. Sepharose 22-31 mitogen-activated protein kinase kinase kinase 20 Homo sapiens 5-8 2165495-4 1990 The NCI-H69 cell alpha 1----3-fucosyltransferase was obtained from a 0.2% Triton X-100-solubilized enzyme fraction after affinity purification on a GDP-hexanolamine-Sepharose column and gel filtration through a fast protein liquid chromatography Superose 12 column. Sepharose 165-174 fucosyltransferase 11 Homo sapiens 17-48 2221385-3 1990 Myosin was covalently tethered to an agarose (Sepharose) support and aliquots of this resin were pipetted onto the surface of the submerged nylon screen. Sepharose 37-44 myosin heavy chain 14 Homo sapiens 0-6 2169238-8 1990 After coupling to Sepharose, the antibody was able to bind to cytochrome P-450(25) from kidney as well as from pig liver microsomes, and to immunoprecipitate the activity for 25-hydroxylation of vitamin D3 and 5 beta-cholestane-3 alpha,7 alpha-diol when assayed in a reconstituted system. Sepharose 18-27 cytochrome P450 family 2 subfamily D member 6 Sus scrofa 62-78 2117920-3 1990 Chromatography of the soluble extract on DEAE-Trisacryl was followed by immunoaffinity chromatography of the complex apolipoprotein AI-binding proteins on anti-(apolipoprotein AI) coupled to Sepharose 4B and then by h.p.l.c. Sepharose 191-200 apolipoprotein A-I Mus musculus 117-134 2117920-3 1990 Chromatography of the soluble extract on DEAE-Trisacryl was followed by immunoaffinity chromatography of the complex apolipoprotein AI-binding proteins on anti-(apolipoprotein AI) coupled to Sepharose 4B and then by h.p.l.c. Sepharose 191-200 apolipoprotein A-I Mus musculus 161-178 2276516-0 1990 Separation of wool intermediate filament protein and intermediate filament-associated protein on a Sepharose column. Sepharose 99-108 repetin Homo sapiens 53-93 2288984-1 1990 A thiol-dependent serine proteinase has been isolated for the first time from a higher basidiomycete Coprinus 7N culture filtrate by affinity chromatography on bacitracin-Sepharose combined with ion-exchange chromatography on DEAE-Sepharose. Sepharose 171-180 endogenous retrovirus group K member 19, envelope Homo sapiens 25-35 2369746-7 1990 On continuous in vitro passage, the CFE in agarose of AA/C1/SB10 has increased to 17.3% and the cells have become tumorigenic producing adenocarcinomas in athymic nude mice. Sepharose 43-50 heterogeneous nuclear ribonucleoprotein C Homo sapiens 57-64 2221385-3 1990 Myosin was covalently tethered to an agarose (Sepharose) support and aliquots of this resin were pipetted onto the surface of the submerged nylon screen. Sepharose 46-55 myosin heavy chain 14 Homo sapiens 0-6 2221385-4 1990 Following addition of radiolabeled protein 4.1, and an appropriate incubation period, the myosin-Sepharose beads and bound protein 4.1 were separated by wicking the buffer from beneath the nylon screen with a piece of filter paper. Sepharose 97-106 myosin heavy chain 14 Homo sapiens 90-96 2227411-6 1990 Ckshs1 or Ckshs2 protein linked to Sepharose beads can bind the Cdc28/Cdc2 protein kinase from both S. cerevisiae and human cells. Sepharose 35-44 CDC28 protein kinase regulatory subunit 1B Homo sapiens 0-6 2201583-2 1990 Human colonic mucin glycoproteins were isolated by Sepharose 4B gel filtration chromatography, they were proteolytically degraded with trypsin, pronase, and papain, and the glycoprotein fractions were reisolated by Sephacryl S-200 gel filtration chromatography. Sepharose 51-60 LOC100508689 Homo sapiens 14-19 2172924-17 1990 Yet, when the two Q-Sepharose fractions were combined, p62 strongly out-competed the otherwise-crosslinkable QB proteins for polypyrimidine tract RNA crosslinking. Sepharose 20-29 nucleoporin 62 Homo sapiens 55-58 2379952-2 1990 Human bronchial mucin from a patient suffering from chronic bronchitis was solubilized in aqueous solution containing sodium azide and protease inhibitors and purified by Sepharose 4B and 2B column chromatography. Sepharose 171-180 LOC100508689 Homo sapiens 16-21 2229028-2 1990 The new glycoprotein was termed heparin binding protein-44 (HBP-44), since it was absorbed to a heparin-agarose column and was eluted from it by a buffer containing 1.5 M NaCl. Sepharose 104-111 low density lipoprotein receptor-related protein associated protein 1 Mus musculus 32-58 2229028-2 1990 The new glycoprotein was termed heparin binding protein-44 (HBP-44), since it was absorbed to a heparin-agarose column and was eluted from it by a buffer containing 1.5 M NaCl. Sepharose 104-111 low density lipoprotein receptor-related protein associated protein 1 Mus musculus 60-66 2275755-0 1990 Thyroxine binding to human serum albumin immobilized on sepharose and effects of nonprotein albumin-binding plasma constituents. Sepharose 56-65 albumin Homo sapiens 27-40 2177163-2 1990 An endopeptidase generating fragment (1-5) from luteinizing hormone-releasing hormone (LHRH) has been solublized with Brij 35 from neuroblastoma cell membrane and purified by a procedure including p-mercuribenzoate-Sepharose chromatography, Mono-Q high-performance liquid chromatography and Superose 6 gel filtration. Sepharose 215-224 gonadotropin releasing hormone 1 Rattus norvegicus 48-85 1696502-2 1990 In the present study, the interaction between PAI-1 and vitronectin has been studied in purified systems and in plasma by agarose gel electrophoresis using non-denaturing conditions and by crossed immunoelectrophoresis using an antiserum produced towards purified PAI-1/vitronectin complex. Sepharose 122-129 serpin family E member 1 Homo sapiens 46-51 2227411-6 1990 Ckshs1 or Ckshs2 protein linked to Sepharose beads can bind the Cdc28/Cdc2 protein kinase from both S. cerevisiae and human cells. Sepharose 35-44 CDC28 protein kinase regulatory subunit 2 Homo sapiens 10-16 2227411-6 1990 Ckshs1 or Ckshs2 protein linked to Sepharose beads can bind the Cdc28/Cdc2 protein kinase from both S. cerevisiae and human cells. Sepharose 35-44 cyclin-dependent serine/threonine-protein kinase CDC28 Saccharomyces cerevisiae S288C 64-69 2113526-2 1990 Aldehyde reductase (ALR1) and aldose reductase (ALR2) were purified from human placenta by a rapid and efficient scheme that included rapid extraction of both reductases from 100,000 x g supernatant material with Red Sepharose followed by purification by chromatofocusing on Pharmacia PBE 94 and then chromatography on a hydroxylapatite high performance liquid chromatography column. Sepharose 217-226 aldo-keto reductase family 1 member A1 Homo sapiens 0-18 1695110-0 1990 Enhanced stimulation of human bone marrow macrophage colony formation in vitro by recombinant human macrophage colony-stimulating factor in agarose medium and at low oxygen tension. Sepharose 140-147 colony stimulating factor 1 Homo sapiens 100-136 2195031-10 1990 The recombinant mature E2b protein was purified to greater than 95% purity in one step using Sepharose 4B column chromatography. Sepharose 93-102 dihydrolipoamide branched chain transacylase E2 Bos taurus 23-26 2113526-2 1990 Aldehyde reductase (ALR1) and aldose reductase (ALR2) were purified from human placenta by a rapid and efficient scheme that included rapid extraction of both reductases from 100,000 x g supernatant material with Red Sepharose followed by purification by chromatofocusing on Pharmacia PBE 94 and then chromatography on a hydroxylapatite high performance liquid chromatography column. Sepharose 217-226 aldo-keto reductase family 1 member B Homo sapiens 30-46 2365078-6 1990 A synthetic peptide corresponding to the insert sequence binds tightly to CAM-Sepharose, demonstrates a shift and enhancement of fluorescence in the presence of CAM, and binds CAM in solution with a KD of 190 nM (in 100 mM KCl). Sepharose 78-87 calmodulin 2 Gallus gallus 74-77 2115553-3 1990 Based on this finding, murine IgD could be rapidly purified directly from whole ascitic fluid by passage over affinity beads of BS-I linked to Sepharose 4B and subsequent elution by a buffer containing 0.1 M D-galactose. Sepharose 143-152 immunoglobulin heavy constant delta Mus musculus 30-33 2391958-1 1990 To procure an affinity gel capable of purifying antibody against the cytochrome P450 component of estrogen synthetase (P450ES), we coupled purified P450ES to agarose supports. Sepharose 158-165 cytochrome P450 family 19 subfamily A member 1 Homo sapiens 98-117 2365078-6 1990 A synthetic peptide corresponding to the insert sequence binds tightly to CAM-Sepharose, demonstrates a shift and enhancement of fluorescence in the presence of CAM, and binds CAM in solution with a KD of 190 nM (in 100 mM KCl). Sepharose 78-87 calmodulin 2 Gallus gallus 161-164 2365078-6 1990 A synthetic peptide corresponding to the insert sequence binds tightly to CAM-Sepharose, demonstrates a shift and enhancement of fluorescence in the presence of CAM, and binds CAM in solution with a KD of 190 nM (in 100 mM KCl). Sepharose 78-87 calmodulin 2 Gallus gallus 161-164 2382764-5 1990 Serum IgE antibody levels are also greater (P less than 0.01) in immunized parasite-challenged mice than in controls (mean cpm 125I-labeled anti-mouse IgE bound to B. malayi antigen-coated Sepharose beads: 7,852 vs. 1,741, respectively). Sepharose 189-198 immunoglobulin heavy constant epsilon Homo sapiens 6-9 2162155-3 1990 alpha-Galactosidase and alpha-N-acetylgalactosaminidase were isolated from Ehrlich ascites tumor cells on epsilon-aminocaproylgalactosylamine-Sepharose. Sepharose 142-151 N-acetyl galactosaminidase, alpha Mus musculus 24-55 2223014-3 1990 Further, GRP78 is a gelatin-binding protein; together with GP46 it is retained on gelatin-Sepharose beads. Sepharose 90-99 heat shock protein family A (Hsp70) member 5 Homo sapiens 9-14 2223014-3 1990 Further, GRP78 is a gelatin-binding protein; together with GP46 it is retained on gelatin-Sepharose beads. Sepharose 90-99 serpin family H member 1 Homo sapiens 59-63 2364492-4 1990 The binding characteristics of thrombin and FPR-thrombin to heparin-Sepharose and to the endothelial surface of rabbit aorta segments in vitro were compared. Sepharose 68-77 prothrombin Oryctolagus cuniculus 31-39 2364492-4 1990 The binding characteristics of thrombin and FPR-thrombin to heparin-Sepharose and to the endothelial surface of rabbit aorta segments in vitro were compared. Sepharose 68-77 prothrombin Oryctolagus cuniculus 48-56 2088636-4 1990 The whole analytical procedure starting from RNA isolation up to agarose gel electrophoresis can be performed in about 5 h. First results indicate that the frequency of the Ph1 translocation exceeds 40 per cent in certain immunologic subtypes of adult acute leukemia. Sepharose 65-72 alanine--glyoxylate and serine--pyruvate aminotransferase Homo sapiens 173-176 2272651-0 1990 Purification of a metallothionein-like protein from serum of mercury-treated rats using phosphorylcholine-Sepharose affinity column. Sepharose 106-115 cysteine and glycine rich protein 3 Rattus norvegicus 18-46 2272651-1 1990 A metallothionein-like protein (MLP) from the serum of mercury-treated rats was isolated while purifying an acute phase plasma protein, C-reactive protein, by its Ca2(+)-dependent affinity to phosphorylcholine (PC)-Sepharose column. Sepharose 215-224 cysteine and glycine rich protein 3 Rattus norvegicus 2-30 2272651-1 1990 A metallothionein-like protein (MLP) from the serum of mercury-treated rats was isolated while purifying an acute phase plasma protein, C-reactive protein, by its Ca2(+)-dependent affinity to phosphorylcholine (PC)-Sepharose column. Sepharose 215-224 cysteine and glycine rich protein 3 Rattus norvegicus 32-35 2272651-1 1990 A metallothionein-like protein (MLP) from the serum of mercury-treated rats was isolated while purifying an acute phase plasma protein, C-reactive protein, by its Ca2(+)-dependent affinity to phosphorylcholine (PC)-Sepharose column. Sepharose 215-224 C-reactive protein Rattus norvegicus 136-154 1695224-7 1990 Both the pituitary holo-hCG- and free hCG-beta-subunit activity could be enriched (approximately 500 times) by affinity chromatography on an hCG antibody-coupled Sepharose column. Sepharose 162-171 chorionic gonadotropin subunit beta 5 Homo sapiens 24-27 1695224-7 1990 Both the pituitary holo-hCG- and free hCG-beta-subunit activity could be enriched (approximately 500 times) by affinity chromatography on an hCG antibody-coupled Sepharose column. Sepharose 162-171 chorionic gonadotropin subunit beta 5 Homo sapiens 38-41 1695224-7 1990 Both the pituitary holo-hCG- and free hCG-beta-subunit activity could be enriched (approximately 500 times) by affinity chromatography on an hCG antibody-coupled Sepharose column. Sepharose 162-171 chorionic gonadotropin subunit beta 5 Homo sapiens 38-41 1695224-9 1990 Like pregnancy hCG, most (75%) of the pituitary hCG was bound to a column of Con A-Sepharose; however, the Con A-nonbinding hCG fraction (approximately 25%) was much higher than that found in pregnancy hCG. Sepharose 83-92 chorionic gonadotropin subunit beta 5 Homo sapiens 48-51 1695224-9 1990 Like pregnancy hCG, most (75%) of the pituitary hCG was bound to a column of Con A-Sepharose; however, the Con A-nonbinding hCG fraction (approximately 25%) was much higher than that found in pregnancy hCG. Sepharose 83-92 chorionic gonadotropin subunit beta 5 Homo sapiens 48-51 1695224-9 1990 Like pregnancy hCG, most (75%) of the pituitary hCG was bound to a column of Con A-Sepharose; however, the Con A-nonbinding hCG fraction (approximately 25%) was much higher than that found in pregnancy hCG. Sepharose 83-92 chorionic gonadotropin subunit beta 5 Homo sapiens 48-51 2116309-7 1990 The recombinant alpha 1----3-galactosyltransferase could be readily detergent-solubilized and subsequently purified by affinity chromatography on UDP-hexanolamine-Sepharose. Sepharose 163-172 N-acetyllactosaminide alpha-1,3-galactosyltransferase Bos taurus 16-50 2224097-0 1990 [Heparin-sepharose affinity chromatography and reduction conditions as a method of a selective process for separate isoforms of apolipoprotein A]. Sepharose 9-18 lipoprotein(a) Homo sapiens 128-144 2197015-12 1990 OMMT has been purified from the livers of irradiated rats by solubilization in high-salt-containing buffer, ammonium sulfate precipitation and a series of column chromatographic steps, including phenyl-Sepharose, heparin-agarose, double-stranded DNA-cellulose and FPLC. Sepharose 221-228 O-6-methylguanine-DNA methyltransferase Rattus norvegicus 0-4 2382764-5 1990 Serum IgE antibody levels are also greater (P less than 0.01) in immunized parasite-challenged mice than in controls (mean cpm 125I-labeled anti-mouse IgE bound to B. malayi antigen-coated Sepharose beads: 7,852 vs. 1,741, respectively). Sepharose 189-198 immunoglobulin heavy constant epsilon Homo sapiens 151-154 2163834-11 1990 Microsomal membrane preparations, solubilized membranes, and receptors purified on an IGF-II-Sepharose column all exhibited stimulation of 125I-IGF-II binding by Man6P, whereas receptors purified on lysosomal enzyme affinity columns showed little or no stimulation of 125I-IGF-II binding by Man6P. Sepharose 93-102 insulin like growth factor 2 Homo sapiens 86-92 2144871-2 1990 Lp[a] was purified from donors with various genetic polymorphic forms by affinity chromatography using lysine-Sepharose or specific immunoadsorbers. Sepharose 110-119 lipoprotein(a) Homo sapiens 0-4 2371272-6 1990 The clofibric acid-Sepharose column could bind HSP70 proteins isolated from various species, which could then be eluted with either clofibric acid or ATP. Sepharose 19-28 heat shock protein family A (Hsp70) member 8 Rattus norvegicus 47-52 2371272-7 1990 Conversely, when a rat liver cytosol containing multiple members of the HSP70 family was passed through an ATP-agarose column, and eluted with clofibric acid, only P72 (HSC70) was eluted. Sepharose 111-118 heat shock protein family A (Hsp70) member 8 Rattus norvegicus 72-77 2402751-3 1990 Intact PF4 bound strongly to heparin agarose and was eluted at 1.4 M NaCl, while reduced PF4 and PF4 C-terminal peptides PF4 (47-70) and PF4 (58-70) bound weakly and were eluted at 0.2-0.5 M NaCl. Sepharose 37-44 platelet factor 4 Homo sapiens 7-10 1696497-1 1990 A galactose-binding lectin (galaptin) from human spleen has been purified to homogeneity by affinity chromatography on asialofetuin-Sepharose. Sepharose 132-141 galectin 1 Homo sapiens 28-36 2111815-8 1990 Specific complex formation between ATP and eIF-2 is shown 1) by effective retention of Met-tRNAf- and mRNA-binding activities on ATP-agarose and by the ability of free ATP, but not GTP, CTP, or UTP, to effect elution of eIF-2 from this substrate; 2) by eIF-2-dependent retention of [alpha-32P]ATP or dATP on nitrocellulose filters and its inhibition by excess ATP, but not by GTP, CTP, or UTP. Sepharose 133-140 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 43-48 2111815-9 1990 Upon elution from ATP-agarose by high salt concentrations, eIF-2 recovers its ability to form a ternary complex with Met-tRNAf and GTP. Sepharose 22-29 eukaryotic translation initiation factor 2 subunit beta Homo sapiens 59-64 2188980-1 1990 A recombinant form of plasminogen activator inhibitor-1 (rPAI-1) has been purified from lysates of pCE1200, a bacterial expression vector containing the full length PAI-1 gene, by utilizing sequential anion exchange and cation exchange chromatography on Q-Sepharose and S-Sepharose columns. Sepharose 256-265 serpin family E member 1 Rattus norvegicus 57-63 2372510-3 1990 The abnormal AT III was purified from the propositus" plasma, taking advantage of the difference in NaCl concentrations required to elute variant and normal AT III from heparin-Sepharose. Sepharose 177-186 serpin family C member 1 Homo sapiens 13-19 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 195-204 colony stimulating factor 2 Homo sapiens 24-30 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 195-204 interleukin 3 Homo sapiens 31-35 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 195-204 ribonuclease A family member 3 Homo sapiens 146-149 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 195-204 endogenous retrovirus group K member 3 Homo sapiens 217-220 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 277-286 colony stimulating factor 2 Homo sapiens 24-30 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 277-286 interleukin 3 Homo sapiens 31-35 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 277-286 ribonuclease A family member 3 Homo sapiens 146-149 2197048-10 1990 On the other hand, when GM-CSF/IL-3-activated eosinophils were exposed to known secretory stimuli, there was a six-fold increase in the amount of ECP released when the cells were stimulated with sepharose coated with C3b, and a two-fold increase when they were stimulated with sepharose-activated whole autologous serum. Sepharose 277-286 endogenous retrovirus group K member 3 Homo sapiens 217-220 2197048-12 1990 A novel finding was that sepharose-activated whole serum was an extremely potent secretory signal for ECP, releasing up to 50% of the total ECP content. Sepharose 25-34 ribonuclease A family member 3 Homo sapiens 102-105 2197048-12 1990 A novel finding was that sepharose-activated whole serum was an extremely potent secretory signal for ECP, releasing up to 50% of the total ECP content. Sepharose 25-34 ribonuclease A family member 3 Homo sapiens 140-143 2357825-0 1990 Age and sex distribution of alkaline phosphatase isoenzymes by agarose electrophoresis. Sepharose 63-70 alkaline phosphatase, placental Homo sapiens 28-48 2357825-1 1990 We separated isoenzymes of alkaline phosphatase (ALP; EC 3.1.3.1) in 1383 sera of normal individuals (ages 4-65 years) by agarose electrophoresis with the Isopal system (Analis). Sepharose 122-129 alkaline phosphatase, placental Homo sapiens 27-47 2357825-1 1990 We separated isoenzymes of alkaline phosphatase (ALP; EC 3.1.3.1) in 1383 sera of normal individuals (ages 4-65 years) by agarose electrophoresis with the Isopal system (Analis). Sepharose 122-129 alkaline phosphatase, placental Homo sapiens 49-52 2351107-3 1990 The bioactivity was eluted in the mol wt region between 40 and 60 K. SIP was further purified by affinity chromatography on blue Sepharose (CL-6B), preparative isoelectrofocusing on sucrose density gradients, anion exchange chromatography on Mono Q by using fast protein liquid chromatography system and gel chromatography on Superose 12. Sepharose 129-138 TSSK6 activating cochaperone Homo sapiens 69-72 1695663-4 1990 The binding of 125I-labelled IGF-I or -II to conditioned (0H) medium was progressively displaced by increasing amounts of unlabelled homologous peptides, while fractionation on concanavalin A-Sepharose showed that the IGF-BPs consisted of both glycoprotein and non-glycoprotein components. Sepharose 192-201 insulin-like growth factor I Ovis aries 29-34 2362447-5 1990 The 90 kDa heat shock protein (hsp90) coimmunoadsorbed with receptors from control cells when protein A-Sepharose pellets were washed with 250 mM NaCl but not when protein A-Sepharose pellets were washed with 500 mM NaCl, indicating that hsp90-receptor complexes are disrupted by a high concentration of salt in the absence of molybdate. Sepharose 104-113 heat shock protein, 2 Mus musculus 31-36 2362447-6 1990 hsp90 coimmunoadsorbed with receptors from RU486-treated cells even when protein A-Sepharose pellets were washed with 500 mM NaCl, indicating that RU486 stabilizes the association of hsp90 with the glucocorticoid receptor. Sepharose 83-92 heat shock protein, 2 Mus musculus 0-5 2214253-2 1990 The fibronectin was purified from autologous and homologous plasma by gelatin-Sepharose 4B affinity chromatography and administered topically, 500 micrograms/ml five times a day, for three weeks. Sepharose 78-87 fibronectin 1 Homo sapiens 4-15 2352928-7 1990 Although we were able to remove IL-3 bioactivity by immunodepletion with anti-IL-3 antibody bound to Sepharose beads, beads with attached HIM1 did not remove IL-3 activity from the medium. Sepharose 101-110 interleukin 3 Homo sapiens 78-82 2352928-7 1990 Although we were able to remove IL-3 bioactivity by immunodepletion with anti-IL-3 antibody bound to Sepharose beads, beads with attached HIM1 did not remove IL-3 activity from the medium. Sepharose 101-110 interleukin 3 Homo sapiens 78-82 2163834-11 1990 Microsomal membrane preparations, solubilized membranes, and receptors purified on an IGF-II-Sepharose column all exhibited stimulation of 125I-IGF-II binding by Man6P, whereas receptors purified on lysosomal enzyme affinity columns showed little or no stimulation of 125I-IGF-II binding by Man6P. Sepharose 93-102 insulin like growth factor 2 Homo sapiens 144-150 2163834-11 1990 Microsomal membrane preparations, solubilized membranes, and receptors purified on an IGF-II-Sepharose column all exhibited stimulation of 125I-IGF-II binding by Man6P, whereas receptors purified on lysosomal enzyme affinity columns showed little or no stimulation of 125I-IGF-II binding by Man6P. Sepharose 93-102 insulin like growth factor 2 Homo sapiens 144-150 2380311-5 1990 Reactive Blue 2 analogues were bonded onto an agarose support matrix by a novel method which entailed immobilisation by the anthraquinone ring 1-amino group as opposed to the usual triazine ring coupling methods. Sepharose 46-53 ring finger protein 1 Equus caballus 138-144 2335508-2 1990 In this assay, aminohexyl lactoside was attached to divinyl sulfone-activated Sepharose, which was then used to bind 125I-galaptin. Sepharose 78-87 galectin 1 Homo sapiens 122-130 2214165-2 1990 Commercially available AT III concentrates showed heterogeneity on agarose gel isoelectric focusing, however, they inhibited thrombin in the same manner. Sepharose 67-74 serpin family C member 1 Homo sapiens 23-29 1978610-1 1990 Gamma-glutamyl transpeptidase was purified to apparent homogeneity from human testis by DEAE cellulose, acetone, precipitation, fractionation by ammonium sulphate, Sephacryl S-200 chromatography, Q-Sepharose chromatography and S-Sepharose chromatography following solubilization of the enzyme by Triton X-100. Sepharose 198-207 inactive glutathione hydrolase 2 Homo sapiens 0-29 2396748-1 1990 A procedure for the isolation of glutamate dehydrogenase (GDH) from human liver, which involves the use of ion-exchange chromatography on diethylaminoethyl cellulose and affinity chromatography on guanosine triphosphate conjugated to Sepharose 4B, is described. Sepharose 234-246 glutamate dehydrogenase 1 Homo sapiens 33-56 2396748-1 1990 A procedure for the isolation of glutamate dehydrogenase (GDH) from human liver, which involves the use of ion-exchange chromatography on diethylaminoethyl cellulose and affinity chromatography on guanosine triphosphate conjugated to Sepharose 4B, is described. Sepharose 234-246 glutamate dehydrogenase 1 Homo sapiens 58-61 1698068-4 1990 After CNBr cleavage and renaturation of aprotinin on CM Sepharose Fast Flow the active aprotinin variant was isolated and its specificities investigated. Sepharose 56-65 pancreatic trypsin inhibitor Bos taurus 40-49 1698068-4 1990 After CNBr cleavage and renaturation of aprotinin on CM Sepharose Fast Flow the active aprotinin variant was isolated and its specificities investigated. Sepharose 56-65 pancreatic trypsin inhibitor Bos taurus 87-96 1694134-1 1990 Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Sepharose 167-176 integrin subunit alpha L Homo sapiens 86-97 2393675-5 1990 Immunochemical relatedness of ferredoxins from bovine adrenocortical and human placental mitochondria allowed one to develop a fast and efficient method for cytochrome P-450scc purification from human placental mitochondria by affinity chromatography on adrenodoxin-Sepharose. Sepharose 266-275 cytochrome P450 family 11 subfamily A member 1 Homo sapiens 157-176 2400590-0 1990 Refolding of proteinase inhibitor variants on trypsin-sepharose as a matrix with complementary structure. Sepharose 54-63 endogenous retrovirus group K member 25 Homo sapiens 13-23 2195055-1 1990 Preparations of IgG 3 isolated by absorption of IgG 1, IgG 2, and IgG 4 from a human iv immunoglobulin with protein A-Sepharose were evaluated for their opsonic activities against type III group B streptococcal (GBS) strains. Sepharose 118-127 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 16-21 1694134-1 1990 Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Sepharose 167-176 integrin subunit alpha L Homo sapiens 105-110 1694134-1 1990 Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Sepharose 167-176 integrin subunit alpha M Homo sapiens 113-128 1694134-1 1990 Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Sepharose 167-176 integrin subunit alpha M Homo sapiens 130-135 1694134-1 1990 Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Sepharose 167-176 integrin subunit beta 2 Homo sapiens 152-156 1694134-1 1990 Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Sepharose 167-176 interleukin 1 alpha Homo sapiens 269-279 1694134-1 1990 Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Sepharose 167-176 interleukin 1 beta Homo sapiens 284-293 1694134-1 1990 Serum-free culture of human monocytes in the presence of monoclonal antibodies to the LFA-1 alpha chain (CD11a), CR3 alpha chain (CD11b) or beta chain (CD18) bound to Sepharose induced the dose-dependent production of cell-associated interleukin (IL) 1 activity and of IL 1 alpha and IL 1 beta antigens, but no release of extracellular IL 1 activity or antigen in the culture medium. Sepharose 167-176 interleukin 1 alpha Homo sapiens 269-273 1692629-8 1990 The binding site for sialic acid appears to reside on factor H, since factor H bound to heparin-agarose and to sialic acid-bearing fetuinagarose, whereas C3b bound to neither under the same conditions. Sepharose 96-103 complement factor H Homo sapiens 54-62 1692629-8 1990 The binding site for sialic acid appears to reside on factor H, since factor H bound to heparin-agarose and to sialic acid-bearing fetuinagarose, whereas C3b bound to neither under the same conditions. Sepharose 96-103 complement factor H Homo sapiens 70-78 2110364-3 1990 Analysis of the patient"s post-heparin plasma by heparin-Sepharose affinity chromatography demonstrated that the mutant LPL had an altered affinity for heparin. Sepharose 57-66 lipoprotein lipase Homo sapiens 120-123 2144382-1 1990 The Ca2+, Mg2(+)-ATPase of the myometrium sarcolemma purified by the method of affinity chromatography on calmodulin sepharose is reconstituted into azolectin liposomes in the functionally active form by means of cholate dialysis. Sepharose 117-126 calmodulin 1 Homo sapiens 106-116 2334436-1 1990 Tartrate-resistant acid phosphatase type-5 was purified to apparent homogeneity from human osteoclastomas by sequential chromatography on CM-Sepharose, Phenyl-Sepharose, concanavalin A-Sepharose, FPLC Superose-12, and FPLC Mono-S. Sepharose 141-150 acid phosphatase 5, tartrate resistant Homo sapiens 0-42 1691174-2 1990 Glycoprotein III (GpIII) was purified from the soluble fraction of bovine chromaffin granules, the secretory vesicles of the adrenal medulla, by chromatography using wheat germ agglutinin-Sepharose followed by reverse-phase high performance liquid chromatography (HPLC). Sepharose 188-197 clusterin Bos taurus 0-16 1691174-2 1990 Glycoprotein III (GpIII) was purified from the soluble fraction of bovine chromaffin granules, the secretory vesicles of the adrenal medulla, by chromatography using wheat germ agglutinin-Sepharose followed by reverse-phase high performance liquid chromatography (HPLC). Sepharose 188-197 clusterin Bos taurus 18-23 2335234-4 1990 In organ cultures resembling closely the in vivo system 10(6) chondrocytes incubated with 100 units of interleukin-1 beta per ml of medium led to the release of 6 X 10(3) units of IL-6 within 24 h. Chondrocytes cultured in agarose or as monolayers similarly incubated with IL-1 beta produced even higher amounts of IL-6: 70 X 10(3) units per 10(6) cells within 24 h. The induction of IL-6 synthesis by IL-1 beta was also shown at the mRNA level. Sepharose 223-230 interleukin 1 beta Homo sapiens 103-121 2335234-4 1990 In organ cultures resembling closely the in vivo system 10(6) chondrocytes incubated with 100 units of interleukin-1 beta per ml of medium led to the release of 6 X 10(3) units of IL-6 within 24 h. Chondrocytes cultured in agarose or as monolayers similarly incubated with IL-1 beta produced even higher amounts of IL-6: 70 X 10(3) units per 10(6) cells within 24 h. The induction of IL-6 synthesis by IL-1 beta was also shown at the mRNA level. Sepharose 223-230 interleukin 6 Homo sapiens 180-184 2182717-1 1990 Human beta 2-microglobulin was isolated from the urine of patients with tubular proteinuria by sequential ion exchange and gel filtration chromatography on Sepharose Q fast flow and Sephacryl S-200 respectively. Sepharose 156-165 beta-2-microglobulin Homo sapiens 6-26 2334436-1 1990 Tartrate-resistant acid phosphatase type-5 was purified to apparent homogeneity from human osteoclastomas by sequential chromatography on CM-Sepharose, Phenyl-Sepharose, concanavalin A-Sepharose, FPLC Superose-12, and FPLC Mono-S. Sepharose 159-168 acid phosphatase 5, tartrate resistant Homo sapiens 0-42 1690727-2 1990 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of gelatin-Sepharose-treated media from BAC cells demonstrated a 2-fold stimulation of fibronectin production by TGF-beta 1 in both the presence and absence of serum. Sepharose 69-78 fibronectin 1 Bos taurus 145-156 1690727-2 1990 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of gelatin-Sepharose-treated media from BAC cells demonstrated a 2-fold stimulation of fibronectin production by TGF-beta 1 in both the presence and absence of serum. Sepharose 69-78 transforming growth factor beta 1 Bos taurus 171-181 2156836-2 1990 During the course of these studies we discovered that a low molecular mass, high affinity GTP-binding protein from bovine brain (designated as the 22-kDa protein) served as an excellent phosphosubstrate for the tyrosine-agarose-purified human placental EGF receptor. Sepharose 220-227 epidermal growth factor receptor Homo sapiens 253-265 2354150-2 1990 While incubation of alpha-thrombin with cathepsin G at pH 7.4 and 37 degrees C resulted in a partial loss of fibrinogen clotting activity, 86 +/- 13% of the clotting activity and 99 +/- 16% of the active sites titratable with p-nitrophenyl p-guanidinobenzoate were retained upon controlled passage of alpha-thrombin through chymotrypsin-Sepharose 4B at pH 6.2 or 7.4 and 24 degrees C (n = 15). Sepharose 337-349 coagulation factor II, thrombin Homo sapiens 26-34 2354150-4 1990 Some 95% of the clotting activity was lost when zeta-thrombin was passed through trypsin-Sepharose 4B under conditions for converting alpha- to nonclotting beta- and subsequently gamma-thrombin. Sepharose 89-98 coagulation factor II, thrombin Homo sapiens 53-61 2354150-4 1990 Some 95% of the clotting activity was lost when zeta-thrombin was passed through trypsin-Sepharose 4B under conditions for converting alpha- to nonclotting beta- and subsequently gamma-thrombin. Sepharose 89-98 coagulation factor II, thrombin Homo sapiens 185-193 2363123-5 1990 This variant AT III was purified, separated from the normal protein by heparin-Sepharose chromatography and was eluted with increased NaCl concentrations. Sepharose 79-88 serpin family C member 1 Homo sapiens 13-19 2363131-0 1990 Luminographic detection of von Willebrand factor multimers in agarose gels and on nitrocellulose membranes. Sepharose 62-69 von Willebrand factor Homo sapiens 27-48 2138609-5 1990 The rHCII was expressed in Escherichia coli and partially purified by heparin-Sepharose chromatography. Sepharose 78-87 serpin family D member 1 Rattus norvegicus 4-9 2317527-8 1990 Release of LPL from heparin-agarose followed a similar pattern, suggesting that the fatty acids specifically affected LPL-heparin interaction. Sepharose 28-35 lipoprotein lipase Bos taurus 11-14 2317527-11 1990 Only the prior incubation with LPL affected the binding to heparin-agarose. Sepharose 67-74 lipoprotein lipase Bos taurus 31-34 2109603-1 1990 Lysosomal neuraminidase (sialidase; EC 3.2.1.18) and beta-galactosidase (EC 3.2.1.23), together with a carboxypeptidase, the so-called "protective protein", were co-purified from the human placenta by affinity chromatography on a concanavalin A-Sepharose column followed by a thiogalactoside-agarose affinity column for beta-galactosidase. Sepharose 245-254 galactosidase beta 1 Homo sapiens 53-71 2109603-1 1990 Lysosomal neuraminidase (sialidase; EC 3.2.1.18) and beta-galactosidase (EC 3.2.1.23), together with a carboxypeptidase, the so-called "protective protein", were co-purified from the human placenta by affinity chromatography on a concanavalin A-Sepharose column followed by a thiogalactoside-agarose affinity column for beta-galactosidase. Sepharose 292-299 galactosidase beta 1 Homo sapiens 53-71 2107883-5 1990 vWF was immunopurified from releasate or media before and after clotting, and the amount and multimeric pattern of vWF bound was determined after sodium dodecyl sulfate agarose gel electrophoresis. Sepharose 169-176 von Willebrand factor Homo sapiens 115-118 2340826-1 1990 A simple agarose isoelectric focusing gel method for apolipoprotein E phenotyping. Sepharose 9-16 apolipoprotein E Homo sapiens 53-69 1970322-5 1990 This was confirmed by depletion of dipeptidyl peptidase IV activity from tissue homogenates by monoclonal antibody OX-61 coupled to Sepharose. Sepharose 132-141 dipeptidylpeptidase 4 Rattus norvegicus 35-58 2138650-6 1990 However, although 125I-Fc epsilon RII bound in a calcium-dependent manner to monosaccharide-agarose beads, high concentrations of mono- and disaccharides did not inhibit the interaction between either 125I-IgE and intact B cells or 125I-Fc epsilon RII (from surface-labeled B cells) and IgE-Sepharose. Sepharose 92-99 Fc receptor, IgE, low affinity II, alpha polypeptide Mus musculus 23-37 2147212-3 1990 The first one uses the extraction of plasma samples with Sep-Pack C18 cartridges and the second immunoextraction (C-terminal ANP-specific antibody bound to solid phase of Sepharose). Sepharose 171-180 natriuretic peptide A Homo sapiens 125-128 2138514-3 1990 Both chimeric receptor chains associate with CD3 polypeptides in functional receptor complexes and respond to phosphorylcholine coupled to Sepharose beads. Sepharose 139-148 CD247 antigen Mus musculus 45-48 2179409-9 1990 A pragmatic result of these newly generated antibodies is the affinity purification to homogeneity of active tryptase by sequential chromatography with B2 coupled to CH-Sepharose and heparin-agarose. Sepharose 191-198 tryptase alpha/beta 1 Mus musculus 109-117 2313094-1 1990 The serum lectin, mannan binding protein (MBP), was isolated in a yield of 40 micrograms/liter from pooled normal human serum by affinity chromatography on mannan-Sepharose, followed by gel-filtration and ion-exchange chromatography and finally by passage down an anti-IgM Sepharose column. Sepharose 163-172 myelin basic protein Homo sapiens 18-40 2313094-1 1990 The serum lectin, mannan binding protein (MBP), was isolated in a yield of 40 micrograms/liter from pooled normal human serum by affinity chromatography on mannan-Sepharose, followed by gel-filtration and ion-exchange chromatography and finally by passage down an anti-IgM Sepharose column. Sepharose 163-172 myelin basic protein Homo sapiens 42-45 2313094-1 1990 The serum lectin, mannan binding protein (MBP), was isolated in a yield of 40 micrograms/liter from pooled normal human serum by affinity chromatography on mannan-Sepharose, followed by gel-filtration and ion-exchange chromatography and finally by passage down an anti-IgM Sepharose column. Sepharose 273-282 myelin basic protein Homo sapiens 18-40 2313094-1 1990 The serum lectin, mannan binding protein (MBP), was isolated in a yield of 40 micrograms/liter from pooled normal human serum by affinity chromatography on mannan-Sepharose, followed by gel-filtration and ion-exchange chromatography and finally by passage down an anti-IgM Sepharose column. Sepharose 273-282 myelin basic protein Homo sapiens 42-45 2313094-4 1990 MBP, after interaction with zymosan, caused efficient activation of a C1r2 125I-C1s2 complex that was prepared by incubation of 125I-C1s2 with serum, from a patient with a complete genetic deficiency of C1q, followed by gel-filtration on Sepharose 6B. Sepharose 238-247 myelin basic protein Homo sapiens 0-3 2138201-3 1990 The GEF and GIF formed by the antigen- or anti-CD3-stimulated T cells had affinity for bee venom phospholipase A2 and could be purified by using anti-lipomodulin Sepharose. Sepharose 162-171 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 4-7 2138201-3 1990 The GEF and GIF formed by the antigen- or anti-CD3-stimulated T cells had affinity for bee venom phospholipase A2 and could be purified by using anti-lipomodulin Sepharose. Sepharose 162-171 cobalamin binding intrinsic factor Homo sapiens 12-15 2140056-5 1990 Toward this end, it was demonstrated that recombinant neuromodulin purified from Escherichia coli and bovine neuromodulin were phosphorylated with similar Km values and stoichiometries and that protein kinase C mediated phosphorylation of both proteins abolished binding to calmodulin-Sepharose. Sepharose 285-294 growth associated protein 43 Bos taurus 54-66 2303491-1 1990 Progesterone receptor complexes were purified from crude cytosol in a rapid, gentle, one-step procedure using anti-receptor monoclonal antibodies covalently attached to an agarose resin. Sepharose 172-179 progesterone receptor Homo sapiens 0-21 2310099-8 1990 The inhibition was blocked by adsorption with Sepharose-coupled cystatin C antibodies and the inhibitor subsequently recovered from the Sepharose beads. Sepharose 46-55 cystatin C Homo sapiens 64-74 2310099-8 1990 The inhibition was blocked by adsorption with Sepharose-coupled cystatin C antibodies and the inhibitor subsequently recovered from the Sepharose beads. Sepharose 136-145 cystatin C Homo sapiens 64-74 1699396-3 1990 Vitronectin was purified from rabbit serum by heparin-Sepharose affinity chromatography and autoclaved at 121 degrees C for 20 min after adjusting the concentration to 0.2 mg/ml with saline. Sepharose 54-63 vitronectin Oryctolagus cuniculus 0-11 2318871-7 1990 By using LCAT covalently bound to Sepharose 4B, a maximal interaction between LTP and bound LCAT was shown to occur at the ionic strength of 0.16. Sepharose 34-43 lecithin-cholesterol acyltransferase Homo sapiens 9-13 2318871-7 1990 By using LCAT covalently bound to Sepharose 4B, a maximal interaction between LTP and bound LCAT was shown to occur at the ionic strength of 0.16. Sepharose 34-43 lecithin-cholesterol acyltransferase Homo sapiens 92-96 2138196-3 1990 At doses ranging from 0.1 to 10 pg/ml, IL-4 suppressed eosinophil secretion of beta-glucuronidase and arylsulfatase by up to 65% after stimulation with IgG-coated Sepharose beads. Sepharose 163-172 interleukin 4 Homo sapiens 39-43 2317200-2 1990 Cytosolic glutathione transferases (GSTs) were purified from the rat spleen by S-hexyl-GSH-Sepharose chromatography, and two major forms were identified as GSTs 2-2 and 7-7 (GST P). Sepharose 91-100 glutathione S-transferase pi 1 Rattus norvegicus 36-40 1690618-0 1990 S protein binds to serum-treated agarose beads independently of complement activation and the formation of the terminal complement complex on the beads. Sepharose 33-40 vitronectin Homo sapiens 0-9 1690618-2 1990 EIA and Western blotting with anti-S protein monoclonal antibody revealed lower S protein values and weaker S protein bands in serum activated by agarose beads than by endotoxin, implying that S protein was removed from serum by binding to agarose. Sepharose 146-153 vitronectin Homo sapiens 35-44 1690618-2 1990 EIA and Western blotting with anti-S protein monoclonal antibody revealed lower S protein values and weaker S protein bands in serum activated by agarose beads than by endotoxin, implying that S protein was removed from serum by binding to agarose. Sepharose 240-247 vitronectin Homo sapiens 35-44 2137090-9 1990 Transferrin-binding ability of MA-PG was studied by affinity column chromatography, using CNBr-activated sepharose bound to transferrin. Sepharose 105-114 transferrin Homo sapiens 0-11 2138034-5 1990 Removal of sialic acids from SAP reduced the calcium-dependent binding activity for agarose by 7%, suggesting the terminal sialic acids were partially responsible for the binding. Sepharose 84-91 amyloid P component, serum Homo sapiens 29-32 2407520-6 1990 True renin in the ocular samples could be separated from nonrenin acid protease by alpha-casein-Sepharose affinity column chromatography at pH 3.5; true renin did not bind to the column, whereas acid protease did. Sepharose 96-105 renin Bos taurus 5-10 1366561-2 1990 IgG-binding activity of ALT was confirmed by Western blotting analysis and by affinity purification with IgG-Sepharose column chromatography. Sepharose 109-118 glutamic pyruvic transaminase, soluble Mus musculus 24-27 2137090-9 1990 Transferrin-binding ability of MA-PG was studied by affinity column chromatography, using CNBr-activated sepharose bound to transferrin. Sepharose 105-114 transferrin Homo sapiens 124-135 2140397-1 1990 Human Lp[a] can be fractionated into two species with different affinities for lysine-Sepharose. Sepharose 86-95 lipoprotein(a) Homo sapiens 6-10 2320389-6 1990 High voltage agarose gel electrophoresis was used to separate free and C8 beta-bound C8 alpha-gamma. Sepharose 13-20 complement C8 beta chain Homo sapiens 71-78 2106684-0 1990 Fractionation of heparin by chromatography on a tissue plasminogen activator-Sepharose column. Sepharose 77-86 chromosome 20 open reading frame 181 Homo sapiens 48-76 2106684-2 1990 To study this interaction, a complex between t-PA and N-acetylated heparin was formed and then linked to Sepharose. Sepharose 105-114 chromosome 20 open reading frame 181 Homo sapiens 45-49 2339364-0 1990 Phosphotungstate as a useful eluent for antithrombin III purification by heparin-agarose affinity chromatography. Sepharose 81-88 serpin family C member 1 Homo sapiens 40-56 2339364-2 1990 In the present paper, we studied whether PTA is useful as an eluent of antithrombin III (ATIII) on heparin-Sepharose affinity chromatography. Sepharose 107-116 serpin family C member 1 Homo sapiens 71-87 2339364-2 1990 In the present paper, we studied whether PTA is useful as an eluent of antithrombin III (ATIII) on heparin-Sepharose affinity chromatography. Sepharose 107-116 serpin family C member 1 Homo sapiens 89-94 2339364-3 1990 Human ATIII adsorbed on the heparin-Sepharose gel was eluted with NaCl buffer at the NaCl level of 1M. Sepharose 36-45 serpin family C member 1 Homo sapiens 6-11 2339367-3 1990 The AT-III deficient plasma is prepared by passage of plasma through a heparin-agarose column. Sepharose 79-86 serpin family C member 1 Homo sapiens 4-10 2310397-2 1990 Inactivated GST P lost its S-hexyl-GSH-Sepharose column affinity. Sepharose 39-48 glutathione S-transferase pi 1 Homo sapiens 12-17 1690780-6 1990 Selected antibodies coupled to Sepharose 4B were used to extract PTH-rP from biological fluids with high yields. Sepharose 31-40 parathyroid hormone like hormone Homo sapiens 65-71 2306116-2 1990 Glutathione reductase (EC 1.6.4.2) isolated from either the bovine intestinal mucosa or the rat liver was bound in a Ca2(+)-dependent manner to oncomodulin which was covalently attached to Sepharose. Sepharose 189-198 glutathione-disulfide reductase Bos taurus 0-21 2306116-2 1990 Glutathione reductase (EC 1.6.4.2) isolated from either the bovine intestinal mucosa or the rat liver was bound in a Ca2(+)-dependent manner to oncomodulin which was covalently attached to Sepharose. Sepharose 189-198 oncomodulin Rattus norvegicus 144-155 2154377-6 1990 125I-VIP-receptor complexes solubilized in Triton X-100 were adsorbed on WGA-Sepharose, extensively washed and the radioactivity retained was eluted with 1 mM GTP showing that: (a) radioactivity corresponds to free 125I-VIP and (b) alpha s (Mr 42,000) and beta (Mr 35,000) subunits of Gs protein are detectable in the GTP eluate by immunoblotting using antisera against these subunits. Sepharose 77-86 vasoactive intestinal peptide Rattus norvegicus 5-8 2154377-7 1990 Such an effect of GTP implied that a stable ternary complex consisting of VIP, receptor and Gs protein had been adsorbed to WGA-Sepharose. Sepharose 128-137 vasoactive intestinal peptide Rattus norvegicus 74-77 2154377-8 1990 When Triton-solubilized 125I-VIP-receptor complexes were adsorbed on WGA-Sepharose, then retained material was specifically eluted with 0.3 M N-acetylglucosamine, analysis of the sugar eluate showed the following results. Sepharose 73-82 vasoactive intestinal peptide Rattus norvegicus 29-32 2187538-4 1990 Monoclonal antibodies coupled to CNBr-Sepharose were used for the rapid one-stage purification of ceruloplasmin from human placental serum. Sepharose 38-47 ceruloplasmin Homo sapiens 98-111 2111182-1 1990 The affinity matrix prepared by the attachment of L-thyroxine (T4) to epichlorohydrine-activated Sepharose 4B biospecifically absorbs the T4-binding globulin (TBG), 25K and 80/27K proteins, immunoglobulin G (IgG) and albumin (HSA) from human normal and retroplacental sera. Sepharose 97-109 serpin family A member 7 Homo sapiens 138-157 2111182-1 1990 The affinity matrix prepared by the attachment of L-thyroxine (T4) to epichlorohydrine-activated Sepharose 4B biospecifically absorbs the T4-binding globulin (TBG), 25K and 80/27K proteins, immunoglobulin G (IgG) and albumin (HSA) from human normal and retroplacental sera. Sepharose 97-109 serpin family A member 7 Homo sapiens 159-162 2153562-2 1990 The activity binds to heparin-Sepharose and it is quenched by polyclonal anti-human placental basic fibroblast growth factor (bFGF) antibodies. Sepharose 30-39 fibroblast growth factor 2 Bos taurus 94-124 2111182-1 1990 The affinity matrix prepared by the attachment of L-thyroxine (T4) to epichlorohydrine-activated Sepharose 4B biospecifically absorbs the T4-binding globulin (TBG), 25K and 80/27K proteins, immunoglobulin G (IgG) and albumin (HSA) from human normal and retroplacental sera. Sepharose 97-109 small nuclear ribonucleoprotein U4/U6.U5 subunit 27 Homo sapiens 176-179 1691960-4 1990 The TSH receptor was purified using affinity chromatography on wheat germ lectin agarose and TSH-agarose. Sepharose 81-88 thyroid stimulating hormone receptor Homo sapiens 4-16 2153562-2 1990 The activity binds to heparin-Sepharose and it is quenched by polyclonal anti-human placental basic fibroblast growth factor (bFGF) antibodies. Sepharose 30-39 fibroblast growth factor 2 Bos taurus 126-130 2153562-3 1990 In the serum-free conditioned medium of FBAE cells, the anti-bFGF antiserum recognizes an immunorective Mr 20,000 molecule which co-purifies with the mitogenic and PA-inducing activity on a heparin-Sepharose column. Sepharose 198-207 fibroblast growth factor 2 Bos taurus 61-65 2159933-1 1990 The HeLa transcription factor LSF has been purified by heparin-agarose and DNA affinity chromatography, and its DNA binding and transcription properties have been characterized. Sepharose 63-70 transcription factor CP2 Homo sapiens 30-33 2108022-2 1990 A novel hyperglycosylated fraction of human term fetal placental fibronectin was detected by long-term affinity binding to gelatin-Sepharose. Sepharose 131-140 fibronectin 1 Homo sapiens 65-76 2323808-1 1990 Human colonic mucin has been isolated from normal colonic mucosa by a phenol-water extraction procedure and purified by Sepharose 2B column chromatography. Sepharose 120-129 LOC100508689 Homo sapiens 14-19 2155244-7 1990 Absorption with agarose containing anti-HLe-1, a mouse monoclonal IgG1 antibody specific for the leukocyte-specific surface protein T-200 (CD45), removed up to 40% of the PTPase activity. Sepharose 16-23 protein tyrosine phosphatase receptor type C Homo sapiens 139-143 2155244-7 1990 Absorption with agarose containing anti-HLe-1, a mouse monoclonal IgG1 antibody specific for the leukocyte-specific surface protein T-200 (CD45), removed up to 40% of the PTPase activity. Sepharose 16-23 cell division cycle 25C Homo sapiens 171-177 2109030-1 1990 Mouse plasma from strains C57BL/6J and C3H/HeJ includes a high density lipoprotein (HDL) fraction containing apolipoprotein A-I which migrates in the prebeta region upon agarose gel electrophoresis, similar to the prebeta HDL previously reported in humans. Sepharose 170-177 apolipoprotein A1 Homo sapiens 109-127 1688916-3 1990 Melanoma-derived fibronectin was isolated from serum-free conditioned medium by gelatin-Sepharose affinity adsorption and shown to react with monoclonal antibodies HNK-1 and 10C5 in Western blot analysis. Sepharose 88-97 fibronectin 1 Homo sapiens 17-28 1688916-4 1990 HNK-1-containing fibronectin was purified on a gelatin-Sepharose column followed by an affinity column using a monoclonal antibody against the HNK-1 carbohydrate. Sepharose 55-64 beta-1,3-glucuronyltransferase 1 Homo sapiens 0-5 1688916-4 1990 HNK-1-containing fibronectin was purified on a gelatin-Sepharose column followed by an affinity column using a monoclonal antibody against the HNK-1 carbohydrate. Sepharose 55-64 fibronectin 1 Homo sapiens 17-28 2300584-8 1990 Model studies with bovine LPL showed that fatty acids displace the enzyme from heparin-agarose. Sepharose 87-94 lipoprotein lipase Bos taurus 26-29 1688812-2 1990 C4B* 15 was distinguished by having only faint staining when using polyclonal anti-C4 antibody on agarose immunoelectrophoresis (e.g. hypomorphic), having relatively weak hemolytic activity but being strongly reactive with monoclonal antibody to Rodgers 1. Sepharose 98-105 complement C4B (Chido blood group) Homo sapiens 0-3 2302411-1 1990 ATP:citrate lyase was purified from the oleaginous yeast Rhodotorula gracilis to homogeneity as judged by polyacrylamide gel electrophoresis, using a novel citrate-Sepharose procedure. Sepharose 164-173 ATP citrate lyase Homo sapiens 0-17 2104849-13 1990 Chromatography studies with heparin-Sepharose indicated that at least some of the lipoprotein lipase in cld/cld cells was dimerized. Sepharose 36-45 lipoprotein lipase Mus musculus 82-100 2303724-2 1990 Complement protein C1q is coupled to Sepharose with an efficiency of 35%, giving 1.7 mg of C1q bound/ml of gel. Sepharose 37-46 complement C1q A chain Homo sapiens 19-22 2303724-2 1990 Complement protein C1q is coupled to Sepharose with an efficiency of 35%, giving 1.7 mg of C1q bound/ml of gel. Sepharose 37-46 complement C1q A chain Homo sapiens 91-94 2337593-2 1990 Human placental NAD(+)-linked 15-hydroxyprostaglandin dehydrogenase was purified to homogeneity according to a five-step method, with chromatography on DEAE-Sepharose, Blue Sepharose, and Mono-Q FPLC as principal steps. Sepharose 157-166 carbonyl reductase 1 Homo sapiens 30-67 2302209-1 1990 Apocarboxypeptidase B-Sepharose was prepared by immobilization of porcine carboxypeptidase B, followed by treatment of the column with o-phenanthrolin. Sepharose 22-31 carboxypeptidase A6 Homo sapiens 3-21 2302209-4 1990 In the apocarboxypeptidase B-Sepharose chromatography, Met-enkephalin-Arg-Arg or dynorphin 1-13 (YGGFLRRIRPKLK), substrates of carboxypeptidase B, was separated from Met-enkephalin (YGGFM), dynorphin B 1-9 (YGGFLRRQF), and beta-neo-endorphin (YGGFLRKYP) which do not react with the immobilized enzyme. Sepharose 29-38 carboxypeptidase A6 Homo sapiens 10-28 2405907-1 1990 We have used three methods to measure the stoichiometry of the glucocorticoid receptor and the 90-kDa heat shock protein (hsp90) in L-cell glucocorticoid receptor complexes that were purified by immunoadsorption to protein A-Sepharose with an anti-receptor monoclonal antibody, followed by a minimal washing procedure that permits retention of receptor-associated protein. Sepharose 225-234 heat shock protein 90 alpha family class A member 1 Homo sapiens 122-127 1688583-3 1990 Antibodies specific to IFN-gamma were affinity purified both from sera taken from healthy individuals and sera from viral-infected patients, by using a rIFN-gamma-coupled CNBr-activated Sepharose 4B column. Sepharose 186-195 interferon gamma Homo sapiens 23-32 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 166-173 insulin Homo sapiens 37-44 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 166-173 insulin Homo sapiens 104-111 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 166-173 insulin Homo sapiens 104-111 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 218-225 insulin Homo sapiens 37-44 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 218-225 insulin Homo sapiens 104-111 2136851-1 1990 In these studies we demonstrate that insulin stimulates both tyrosine and serine phosphorylation of the insulin receptor after its partial purification on wheat germ-agarose, and after affinity purification on insulin-agarose. Sepharose 218-225 insulin Homo sapiens 104-111 2295605-2 1990 The purification procedure involved DEAE-Sepharose CL-6B (to remove calmodulin), calmodulin-Sepharose 4B affinity, and Sepharose 6B column chromatographies. Sepharose 92-101 calmodulin Bos taurus 81-91 2403558-14 1990 Affinity chromatography of surface-labeled cell extracts on fibrinogen-Sepharose revealed a 94 +/- 2-kDa membrane protein that bound specifically to fibrinogen-Sepharose only on cells that expressed the MFR. Sepharose 71-80 fibrinogen beta chain Homo sapiens 60-70 2302178-3 1990 The protein has been isolated from plasma by immunoaffinity chromatography on an anti-(erythrocyte MIP)-Sepharose column and shown by SDS/polyacrylamide-gel electrophoresis to be of similar molecular mass to the erythrocyte protein (55 kDa non-reduced and 65 kDa under reducing conditions). Sepharose 104-113 major intrinsic protein of lens fiber Homo sapiens 99-102 1965083-1 1990 To study the mechanisms of activation of human neutrophil gelatinase, the enzyme has been purified using a combination of chromatography on a DEAE-Sephacel and a gelatin-peptide-Sepharose column. Sepharose 178-187 matrix metallopeptidase 2 Homo sapiens 47-68 1706557-5 1990 Mitogenic activity in the 1.2 M NaCl fraction of Heparin-Sepharose chromatography suggests the presence of acidic FGF (aFGF). Sepharose 57-66 fibroblast growth factor 1 Homo sapiens 107-117 1706557-5 1990 Mitogenic activity in the 1.2 M NaCl fraction of Heparin-Sepharose chromatography suggests the presence of acidic FGF (aFGF). Sepharose 57-66 fibroblast growth factor 1 Homo sapiens 119-123 2117407-1 1990 We describe a method for agarose IEF under acid conditions in which a single gel can be used to diagnose from equine red cell lysates genetic variants for carbonic anhydrase (CA) and catalase (Cat). Sepharose 25-32 catalase Equus caballus 193-196 2288445-1 1990 A luminescent adsorbent constituted of bacterial luciferase, FMN oxidoreductase and a protein, such as an antibody or an oligonucleotide coimmobilized on Sepharose, has been used to detect a label enzyme (Glucose 6 phosphate dehydrogenase). Sepharose 154-163 glucose-6-phosphate dehydrogenase Homo sapiens 205-238 2136995-7 1990 WGA-agarose chromatography of CHAPS soluble hippocampal extract supplemented with GTP allowed the physical separation of R[5-HT1A] from the bulk of G proteins, and a concomitant decrease of [3H]8-OH-DPAT high affinity binding capacity. Sepharose 4-11 5-hydroxytryptamine receptor 1A Rattus norvegicus 123-129 2403558-14 1990 Affinity chromatography of surface-labeled cell extracts on fibrinogen-Sepharose revealed a 94 +/- 2-kDa membrane protein that bound specifically to fibrinogen-Sepharose only on cells that expressed the MFR. Sepharose 71-80 fibrinogen beta chain Homo sapiens 149-159 2403558-14 1990 Affinity chromatography of surface-labeled cell extracts on fibrinogen-Sepharose revealed a 94 +/- 2-kDa membrane protein that bound specifically to fibrinogen-Sepharose only on cells that expressed the MFR. Sepharose 160-169 fibrinogen beta chain Homo sapiens 60-70 2403558-14 1990 Affinity chromatography of surface-labeled cell extracts on fibrinogen-Sepharose revealed a 94 +/- 2-kDa membrane protein that bound specifically to fibrinogen-Sepharose only on cells that expressed the MFR. Sepharose 160-169 fibrinogen beta chain Homo sapiens 149-159 2403558-14 1990 Affinity chromatography of surface-labeled cell extracts on fibrinogen-Sepharose revealed a 94 +/- 2-kDa membrane protein that bound specifically to fibrinogen-Sepharose only on cells that expressed the MFR. Sepharose 160-169 signal regulatory protein alpha Homo sapiens 203-206 2140680-1 1990 Modifications to the commonly employed lysine Sepharose affinity chromatography method for the purification of plasminogen from human plasma, give a preparation of native, Glu-plasminogen which is free of plasmin contamination. Sepharose 46-55 plasminogen Homo sapiens 111-118 2140680-0 1990 Modifications to the lysine Sepharose method of plasminogen purification which ensure plasmin-free Glu-plasminogen. Sepharose 28-37 plasminogen Homo sapiens 48-55 3918107-3 1985 All macrophage-activating factor detected by these assays was also removed by passing the lymphokines over a Sepharose column to which the monoclonal anti-IFN-gamma antibody had been coupled. Sepharose 109-118 interferon gamma Mus musculus 155-164 778444-2 1975 MIF was released from mouse B cells when stimulated by Sepharose-PWM but was not released by Sepharose-Con A. Sepharose 55-64 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 0-3 778444-3 1975 MIF was produced by the low density subpopulation of thymus cells stimulated by Sepharose-Con A. Sepharose 80-89 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 0-3 33973097-3 2021 The objective of this work was to test the functionality of the strategy of Rational Design of Immobilized Derivatives for the immobilization by electrostatic adsorption of egg white lysozyme on SP-Sepharose FastFlow support. Sepharose 198-207 lysozyme Homo sapiens 183-191 33973097-7 2021 The enzymatic activity assay with the chitosan substrate showed the catalytic functionality of the lysozyme-SP-Sepharose immobilized derivative (35.85 +- 3.07 U/support g), which preserved 78% functional activity. Sepharose 111-120 lysozyme Homo sapiens 99-107 2194572-9 1990 It is shown that ACP-Sepharose may be used as a matrix in the purification of enoyl-ACP reductase. Sepharose 21-30 enoyl-[acyl-carrier-protein] reductase [NADH], chloroplastic-like Brassica napus 78-97 34954298-4 2022 Agarose native gel electrophoresis showed that aggregation of bovine serum albumin (BSA) induced by heating was slightly reduced by NaCl and ArgHCl. Sepharose 0-7 albumin Homo sapiens 69-82 2083237-4 1990 Lactoferrin interacts with beta-lactoglobulin-Sepharose at low ionic strength, but not in the presence of 0.3 M NaCl, indicating that ionic interactions are important. Sepharose 46-55 lactotransferrin Bos taurus 0-11 2083237-4 1990 Lactoferrin interacts with beta-lactoglobulin-Sepharose at low ionic strength, but not in the presence of 0.3 M NaCl, indicating that ionic interactions are important. Sepharose 46-55 beta-lactoglobulin Bos taurus 27-45 2083241-6 1990 Fibronectin could also dissociate LDL-heparin complexes formed on heparin-Sepharose affinity columns. Sepharose 74-83 fibronectin 1 Homo sapiens 0-11 2302436-4 1990 We demonstrate that this activity actually corresponds to LPL, since it is inhibited by either 1.5 M NaCl or 1.5 mg/ml protamine sulfate, is serum-dependent, and could be separated from hepatic lipase activity by using heparin-Sepharose affinity chromatography. Sepharose 227-236 lipoprotein lipase Rattus norvegicus 58-61 2344455-1 1990 Using affinity chromatography on heparin-Sepharose 4B, triglyceride lipase was isolated from rabbit liver tissue and purified. Sepharose 41-50 pancreatic triacylglycerol lipase Oryctolagus cuniculus 55-74 2322938-4 1990 The TNF activity in the sera of mice was abrogated almost completely by anti-(murine TNF alpha) antibody with protein-A-agarose. Sepharose 120-127 tumor necrosis factor Mus musculus 4-7 2322938-4 1990 The TNF activity in the sera of mice was abrogated almost completely by anti-(murine TNF alpha) antibody with protein-A-agarose. Sepharose 120-127 tumor necrosis factor Mus musculus 85-94 2073889-6 1990 Oxidase activity of ceruloplasmin was determined by 01 todianisidine, but sera proteins-by agarose electrophoresis. Sepharose 91-98 ceruloplasmin Cavia porcellus 20-33 2318192-0 1990 Haptoglobin subtype determination by isoelectric focusing in agarose gel: application to paternity testing and presentation of a new alpha 2-variant. Sepharose 61-68 haptoglobin Homo sapiens 0-11 2322473-7 1990 The differences in the yields between IgG3s carrying various haplotypes on Protein A-Sepharose affinity chromatography [Ito et al. Sepharose 85-94 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 38-42 1694154-4 1990 But PAPP-A isolated by heparin-Sepharose chromatography, or a PAPP-A-free washing of the heparin-Sepharose column were both inhibitors of HLE. Sepharose 31-40 pappalysin 1 Homo sapiens 4-10 1694154-4 1990 But PAPP-A isolated by heparin-Sepharose chromatography, or a PAPP-A-free washing of the heparin-Sepharose column were both inhibitors of HLE. Sepharose 97-106 elastase, neutrophil expressed Homo sapiens 138-141 1694154-5 1990 Furthermore the inactive PAPP-A preparation, when incubated with the PAPP-A-free washing of the heparin-Sepharose column, yielded a high molecular weight preparation which inhibited HLE. Sepharose 104-113 pappalysin 1 Homo sapiens 25-31 1694154-5 1990 Furthermore the inactive PAPP-A preparation, when incubated with the PAPP-A-free washing of the heparin-Sepharose column, yielded a high molecular weight preparation which inhibited HLE. Sepharose 104-113 pappalysin 1 Homo sapiens 69-75 1694154-5 1990 Furthermore the inactive PAPP-A preparation, when incubated with the PAPP-A-free washing of the heparin-Sepharose column, yielded a high molecular weight preparation which inhibited HLE. Sepharose 104-113 elastase, neutrophil expressed Homo sapiens 182-185 2101805-9 1990 The percent of cells bound on SpA-Sepharose varied between 6% and 66%, representing the hairy cells with labile-bound smIgG. Sepharose 34-43 surfactant protein A1 Homo sapiens 30-33 2129163-3 1990 FVIII-deficient plasma was prepared by immunodepletion using polyclonal anti-von-Willebrand factor (vWF) and monoclonal anti-FVIII antibodies coated to agarose beads. Sepharose 152-159 coagulation factor VIII Homo sapiens 0-5 2272662-3 1990 Certain alleles (DR3 and DR4) could be rapidly and accurately identified by running the products of allele-specific amplification of genomic DNA on agarose gels. Sepharose 148-155 TNF receptor superfamily member 25 Homo sapiens 17-20 2272662-3 1990 Certain alleles (DR3 and DR4) could be rapidly and accurately identified by running the products of allele-specific amplification of genomic DNA on agarose gels. Sepharose 148-155 major histocompatibility complex, class II, DR beta 4 Homo sapiens 25-28 2149066-0 1990 Inhibitory effect of IL-4 on the sepharose-CD3-induced proliferation of the CD4CD45RO human T cell subset. Sepharose 33-42 interleukin 4 Homo sapiens 21-25 2149066-4 1990 We have found that both cell subsets are able to proliferate after stimulation with Sepharose-CD3 in the presence of externally added IL-2 or IL-4. Sepharose 84-93 interleukin 2 Homo sapiens 134-138 2149066-4 1990 We have found that both cell subsets are able to proliferate after stimulation with Sepharose-CD3 in the presence of externally added IL-2 or IL-4. Sepharose 84-93 interleukin 4 Homo sapiens 142-146 2149066-6 1990 Both cell subsets showed a good response to Sepharose-CD3 plus adherent cells (AC), but when IL-4 was present in the culture only the CD4CD45RA cells showed an enhancement in the Sepharose-CD3-induced proliferation, while proliferation of the CD4CD45RO T cell subset was inhibited. Sepharose 179-188 interleukin 4 Homo sapiens 93-97 2157779-5 1990 The GM-CSF receptor is a glycoprotein which binds to wheat germ agglutinin-sepharose. Sepharose 75-84 colony stimulating factor 2 Homo sapiens 4-10 1690279-1 1990 alpha-Fetoprotein in sera from healthy subjects and patients with liver cirrhosis and hepatocellular carcinoma was fractionated into three peaks by affinity chromatography on a column of Lens culinaris agglutinin-Sepharose 4B. Sepharose 213-222 alpha fetoprotein Homo sapiens 0-17 1693671-1 1990 Alpha-fetoprotein in sera from patients with hepatocellular carcinoma was fractionated into three peaks by affinity chromatography on a column of Lens culinaris agglutinin-Sepharose 4B. Sepharose 172-181 alpha fetoprotein Homo sapiens 0-17 2153700-4 1990 After PCR amplification of B19 DNA in serum, a 699-nucleotide DNA fragment was detected on agarose gels. Sepharose 91-98 eva-1 homolog C Homo sapiens 27-30 2136879-1 1990 Soluble polymerized human C3b and C3b bound to Sepharose induced the production of cell-associated IL-1 and the extracellular release of IL-1 activity from cultured human adherent monocytes. Sepharose 47-56 complement C3 Homo sapiens 26-29 2136879-1 1990 Soluble polymerized human C3b and C3b bound to Sepharose induced the production of cell-associated IL-1 and the extracellular release of IL-1 activity from cultured human adherent monocytes. Sepharose 47-56 complement C3 Homo sapiens 34-37 2136879-1 1990 Soluble polymerized human C3b and C3b bound to Sepharose induced the production of cell-associated IL-1 and the extracellular release of IL-1 activity from cultured human adherent monocytes. Sepharose 47-56 interleukin 1 beta Homo sapiens 99-103 2136879-1 1990 Soluble polymerized human C3b and C3b bound to Sepharose induced the production of cell-associated IL-1 and the extracellular release of IL-1 activity from cultured human adherent monocytes. Sepharose 47-56 interleukin 1 beta Homo sapiens 137-141 2136880-7 1990 The relative efficacy of mAb against intact C4BP in blocking C4BP binding to heparin-Sepharose was similar to that for blocking 125I-C4BP binding to C4b. Sepharose 85-94 complement component 4 binding protein alpha Homo sapiens 44-48 2136880-7 1990 The relative efficacy of mAb against intact C4BP in blocking C4BP binding to heparin-Sepharose was similar to that for blocking 125I-C4BP binding to C4b. Sepharose 85-94 complement component 4 binding protein alpha Homo sapiens 61-65 2136880-7 1990 The relative efficacy of mAb against intact C4BP in blocking C4BP binding to heparin-Sepharose was similar to that for blocking 125I-C4BP binding to C4b. Sepharose 85-94 complement component 4 binding protein alpha Homo sapiens 61-65 1964384-8 1990 Using two-stage assays, we determined that ACTH acts during the latter stages of B cell activation (i.e., 3-4 days after initial stimulation with either the combination of IL-4, GAMIg-Sepharose, and IL-1 or the combination of DxS and IL-5). Sepharose 184-193 pro-opiomelanocortin-alpha Mus musculus 43-47 2175810-0 1990 Binding of lysosomal enzymes to the mannose 6-phosphate receptor: a novel binding assay that makes use of biotinylated receptor molecules, coupled to avidin-agarose. Sepharose 157-164 progesterone receptor membrane component 1 Rattus norvegicus 36-64 2175810-5 1990 We therefore developed a method in which MPR was biotinylated, followed by coupling to avidin-agarose. Sepharose 94-101 progesterone receptor membrane component 1 Rattus norvegicus 41-44 2104544-4 1990 These two genes recombined with wild-type virus genome to yield recombinants which were polyhedrin negative, produced the foreign gene product, and formed blue plaques when beta-galactosidase indicator was present in the agarose overlay. Sepharose 221-228 galactosidase beta 1 Homo sapiens 173-191 27457296-3 1990 The specificity of the amplification allowed visual identification of the bcl-2/JH PCR-products in ethidium bromide stained agarose gels. Sepharose 124-131 BCL2 apoptosis regulator Homo sapiens 74-79 2159089-6 1990 The combination of horizontal discontinuous agarose slab gel and Southern hybridization results show CG2 and CG3 cells are of monoclonal origin and harbor episomal EBV genomes. Sepharose 44-51 transmembrane protein 245 Homo sapiens 101-104 1700409-1 1990 Immunoreactive substance P was recovered from human brain (hypothalamus and substantia nigra) by acetic acid extraction, ion exchange chromatography (SP-Sephadex), molecular sieving (Sephadex G-50) and column electrophoresis in agarose suspension. Sepharose 228-235 tachykinin precursor 1 Homo sapiens 15-26 2287610-8 1990 By our method, the DEAE-Sephadex step is omitted, the G6PD is eluted from P11 with citrate and NADP, and from 2"5" ADP-Sepharose with KC1, NADP and EDTA. Sepharose 119-128 glucose-6-phosphate dehydrogenase Homo sapiens 54-58 2349208-1 1990 The effects of temperature, pH, and concentration of sodium cacodylate buffer on the activity of partially purified terminal deoxynucleotidyl transferase from cattle thymus immobilized on BrCN-Sepharose were studied. Sepharose 193-202 DNA nucleotidylexotransferase Bos taurus 116-153 2367346-0 1990 Purification of nodule-specific uricase from soybean by arginine-sepharose affinity chromatography. Sepharose 65-74 uricase-2 isozyme 1 Glycine max 32-39 2367346-3 1990 Crude extracts were loaded onto small columns of Arginine-Sepharose and a significant retardation of uricase was observed. Sepharose 58-67 uricase-2 isozyme 1 Glycine max 101-108 2392481-5 1990 Agarose-IEF analyses, substrate specificity studies and immunochemical titrations of the major kidney ADH (ADH-1) also support the occurrence of a second Class I isozyme. Sepharose 0-7 alcohol dehydrogenase 1A (class I), alpha polypeptide Homo sapiens 102-105 2169057-1 1990 In an attempt to identify collagen-binding proteins on the chondrocyte surface, a protein of Mr 34KD, called Anchorin CII was isolated from chondrocyte membranes by affinity chromatography on type II collagen sepharose (Mollenhauer & von der Mark, 1983). Sepharose 209-218 annexin A5 Homo sapiens 109-121 1689072-7 1990 Presence of co-cultured agarose beads reduced the amount of soluble S-protein due to deposition on the agarose. Sepharose 24-31 vitronectin Homo sapiens 68-77 1689072-7 1990 Presence of co-cultured agarose beads reduced the amount of soluble S-protein due to deposition on the agarose. Sepharose 103-110 vitronectin Homo sapiens 68-77 1689072-9 1990 In the fluid phase, SC5b-9 was generated, whereas the agarose-bound S-protein is assumed not to be associated with TCC on the beads. Sepharose 54-61 vitronectin Homo sapiens 68-77 2291319-2 1990 AC with the use of specific proteinase plasma sorption in a column with sepharose-immobilized acid-base proteinase inhibitor from human urine prevents activation of proteinase in the plasma. Sepharose 72-81 endogenous retrovirus group K member 25 Homo sapiens 28-38 2291319-2 1990 AC with the use of specific proteinase plasma sorption in a column with sepharose-immobilized acid-base proteinase inhibitor from human urine prevents activation of proteinase in the plasma. Sepharose 72-81 endogenous retrovirus group K member 25 Homo sapiens 104-114 2291319-2 1990 AC with the use of specific proteinase plasma sorption in a column with sepharose-immobilized acid-base proteinase inhibitor from human urine prevents activation of proteinase in the plasma. Sepharose 72-81 endogenous retrovirus group K member 25 Homo sapiens 104-114 2091384-2 1990 The specific sensitization to human heart myosin was examined by two methods concurrently: method of inhibiting leucocyte migration in agarose and method for direct specific lymphocytolysis with myosin. Sepharose 135-142 myosin heavy chain 14 Homo sapiens 42-48 33774457-0 2021 Ligand screening assay for the enzyme kallikrein immobilized on NHS-activated Sepharose. Sepharose 78-87 kallikrein related peptidase 4 Homo sapiens 38-48 33774457-0 2021 Ligand screening assay for the enzyme kallikrein immobilized on NHS-activated Sepharose. Sepharose 78-87 NHS actin remodeling regulator Homo sapiens 64-67 33774457-3 2021 In this study, we have covalently immobilized porcine pancreas KLK on an NHS-activated Sepharose matrix, to obtain KLK-Sepharose-NHS. Sepharose 87-96 NHS actin remodeling regulator Homo sapiens 73-76 33774457-3 2021 In this study, we have covalently immobilized porcine pancreas KLK on an NHS-activated Sepharose matrix, to obtain KLK-Sepharose-NHS. Sepharose 87-96 NHS actin remodeling regulator Homo sapiens 129-132 33774457-3 2021 In this study, we have covalently immobilized porcine pancreas KLK on an NHS-activated Sepharose matrix, to obtain KLK-Sepharose-NHS. Sepharose 119-128 NHS actin remodeling regulator Homo sapiens 73-76 33774457-3 2021 In this study, we have covalently immobilized porcine pancreas KLK on an NHS-activated Sepharose matrix, to obtain KLK-Sepharose-NHS. Sepharose 119-128 NHS actin remodeling regulator Homo sapiens 129-132 33774457-5 2021 As proof of concept, we used leupeptin as a reference inhibitor to perform inhibition studies for KLK-Sepharose-NHS and to determine the half-maximal inhibitory concentration (IC50 = 0.13 +- 0.01 muM), the inhibition constant (Ki = 0.06 muM), and the leupeptin inhibition mechanism. Sepharose 102-111 NHS actin remodeling regulator Homo sapiens 112-115 33774457-8 2021 KLK-Sepharose-NHS was fully active after six consecutive reaction cycles and retained about 60 % of its initial activity after being used for at least five months, so the bioassay developed herein is a promising strategy to screen and to identify KLK ligands. Sepharose 4-13 NHS actin remodeling regulator Homo sapiens 14-17 9063496-0 1997 Clusterin and the terminal complement pathway synthesized by human umbilical vein endothelial cells are closely linked when detected on co-cultured agarose beads. Sepharose 148-155 clusterin Homo sapiens 0-9 9063496-1 1997 Clusterin and the terminal complement pathway synthesized by human umbilical vein endothelial cells are closely linked when detected on co-cultured agarose beads. Sepharose 148-155 clusterin Homo sapiens 0-9 9063496-3 1997 The aim of the present study was to examine whether clusterin was an integral part of serum-derived TCC bound to agarose beads which activate the alternative pathway of complement. Sepharose 113-120 clusterin Homo sapiens 52-61 9063496-6 1997 We found that clusterin in serum experiments is bound to C9 in agarose bound TCC and not directly to the agarose. Sepharose 63-70 clusterin Homo sapiens 14-23 9063496-8 1997 The results indicate that clusterin derived from the EC is linked with the TCC on the co-incubated beads for the following reasons: First, in serum experiments clusterin like vitronectin, was co-deposited with C9 in agarose-bound TCC. Sepharose 216-223 clusterin Homo sapiens 26-35 9063496-8 1997 The results indicate that clusterin derived from the EC is linked with the TCC on the co-incubated beads for the following reasons: First, in serum experiments clusterin like vitronectin, was co-deposited with C9 in agarose-bound TCC. Sepharose 216-223 vitronectin Homo sapiens 175-186 2019321-2 1991 Agarose beads were soaked in solutions containing TGF-beta 1 and implanted into wing buds at stages 18 to 27. Sepharose 0-7 transforming growth factor beta 1 Gallus gallus 50-60 34875538-4 2022 Two novel deletion mutations were identified in intronic region of Cry2 gene and were genotyped by agarose gel electrophoresis and DNA sequencing. Sepharose 99-106 cryptochrome-2 Ovis aries 67-71 34816539-6 2022 HMGB1 bound to the upstream promoter region of Foxp1 as demonstrated by the dual-luciferase reporter assay, ChIP-qPCR and agarose gel electrophoresis. Sepharose 122-129 high mobility group box 1 Mus musculus 0-5 34816539-6 2022 HMGB1 bound to the upstream promoter region of Foxp1 as demonstrated by the dual-luciferase reporter assay, ChIP-qPCR and agarose gel electrophoresis. Sepharose 122-129 forkhead box P1 Mus musculus 47-52 34633097-1 2022 Nucleic acids dye Goldview is widely used in agarose gel electrophoresis (AGE). Sepharose 45-52 renin binding protein Homo sapiens 74-77 34543954-3 2021 All the AR E. coli (~8 log) was inactivated and 8.17 log blaTEM-1 was degraded by the carbon nanotubes/agarose/titanium (CNTs/AG/Ti) electrode within 30 min. Sepharose 103-110 beta-lactamase Escherichia coli 57-65 34417692-6 2022 Agarose gel electrophoresis revealed DNA fragmentations in OSL and NPL-treated cells. Sepharose 0-7 N-acetylneuraminate pyruvate lyase Homo sapiens 67-70 34391728-5 2021 Important features of the hNit2 gene within the pQE-Nit22 construct are: 1) optimized codon composition, 2) the presence of an N-terminus His6 tag immediately after the initiating codon ATG (Met) that permits efficient purification of the end-product on a Ni-NTA-agarose column. Sepharose 263-270 nitrilase family member 2 Homo sapiens 26-31 34115923-10 2021 Agarose had an inhibitory effect on MPO release and fibrin a retarding effect on NETosis. Sepharose 0-7 myeloperoxidase Homo sapiens 36-39 34777931-7 2021 The developed functional marker was capable of identifying the allelic status at OVATE locus in a co-dominant manner, using routine polymerase chain reaction (PCR) followed by standard agarose gel electrophoresis. Sepharose 185-192 OVATE family protein Solanum lycopersicum 81-86 34845785-9 2022 A clone of chicken HspB1 in BL21 (DE3) cells was revived, and recombinant HspB1 was purified using Ni-NTA agarose column. Sepharose 106-113 heat shock protein family B (small) member 1 Homo sapiens 74-79 34577715-0 2021 Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications. Sepharose 37-44 pyruvate carboxylase Homo sapiens 22-25 34723292-3 2021 In this paper, we carried out an innovative study on the ability of bovine serum albumin stabilized gold nanoclusters (BSA-AuNCs) to perform as reliable label-free contrast agents for the visualization of tissue-like agarose phantoms via TPE-FLIM. Sepharose 217-224 albumin Homo sapiens 75-88 34625786-0 2021 Purification of Recombinant Mouse C-Reactive Protein from Pichia Pastoris GS115 by Nickel Chelating Sepharose Fast-Flow Affinity Chromatography and P-Aminophenyl Phosphoryl Choline Agarose Resin Affinity Chromatography in Tandem. Sepharose 100-109 C-reactive protein, pentraxin-related Mus musculus 34-52 34711005-7 2021 The PCR products of all the exons of TP53 (Exons 2 to 11) were electrophoresed on agarose gel, purified and sequenced by Sanger method. Sepharose 82-89 tumor protein p53 Homo sapiens 37-41 34498771-4 2021 The MTHFR C677T genotyping was conducted by PCR-RFLP followed by agarose gel electrophoresis. Sepharose 65-72 methylenetetrahydrofolate reductase Homo sapiens 4-9 34310055-5 2021 Furthermore, significant increases in cytoplasmic maturation-related parameters (BMP15 level, cumulus cell expansion score, intra-oocyte ATP level, and index of cortical granule distribution) were observed in COCs matured in vitro using ECM protein-incorporated agarose matrices. Sepharose 262-269 bone morphogenetic protein 15 Homo sapiens 81-86 34568727-3 2021 The size distribution of VWF multimers is usually analyzed by SDS-agarose gel electrophoresis followed by immunoblotting. Sepharose 66-73 von Willebrand factor Homo sapiens 25-28 34519921-6 2022 This assay yielded a limit of detection of 500 copies/mL, making it more sensitive than traditional bioluminescent assays, about 1000 times more sensitive than that of PCR product analysis by agarose gel electrophoresis, and roughly as sensitive as qPCR as a means of detecting viral DNA. Sepharose 192-199 thrombopoietin Mus musculus 54-56 34577715-1 2021 In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. Sepharose 62-69 pyruvate carboxylase Homo sapiens 54-57 34221965-4 2021 A streptavidin agarose affinity assay was used to verify the direct interaction between P7C3 and phosphoglycerate kinase 1 (PGK1). Sepharose 15-22 phosphoglycerate kinase 1 Homo sapiens 97-122 34407819-10 2021 The SED1 signal peptide derived from Saccharomyces cerevisiae was selected as optimal for extracellular production of NeoDP4 from agarose. Sepharose 130-137 Sed1p Saccharomyces cerevisiae S288C 4-8 34471403-14 2021 Using agarose plug transplants in mice harbouring P-I metalloproteinases and LAAO we demonstrated a marked disruption of vasculature at the periphery. Sepharose 6-13 interleukin 4 induced 1 Mus musculus 77-81 34351836-3 2021 For this aim, the GST enzyme was purified from Vaccinium arctostapylous L. using the glutathione-agarose affinity chromatography and Sephadex G-100 gel filtration steps. Sepharose 97-104 glutathione S-transferase kappa 1 Homo sapiens 18-21 34082094-4 2021 As a proof-of-concept study, we preloaded hepatic growth factor (HGF) into the MXene@hydrogel (MXene@agarose/HGF) to activate the c-Met-mediated signaling by NIR light. Sepharose 101-108 hepatocyte growth factor Homo sapiens 42-63 34082094-4 2021 As a proof-of-concept study, we preloaded hepatic growth factor (HGF) into the MXene@hydrogel (MXene@agarose/HGF) to activate the c-Met-mediated signaling by NIR light. Sepharose 101-108 hepatocyte growth factor Homo sapiens 65-68 34082094-4 2021 As a proof-of-concept study, we preloaded hepatic growth factor (HGF) into the MXene@hydrogel (MXene@agarose/HGF) to activate the c-Met-mediated signaling by NIR light. Sepharose 101-108 hepatocyte growth factor Homo sapiens 109-112 34082094-4 2021 As a proof-of-concept study, we preloaded hepatic growth factor (HGF) into the MXene@hydrogel (MXene@agarose/HGF) to activate the c-Met-mediated signaling by NIR light. Sepharose 101-108 MET proto-oncogene, receptor tyrosine kinase Homo sapiens 130-135 34082094-6 2021 Our approach"s versatility was validated by preloading tumor necrotic factor-alpha (TNF-alpha) into the composite hydrogel (MXene@agarose/TNF-alpha) to promote the pro-apoptotic signaling pathway, achieving the NIR light-induced programmed cell deaths (PCD) of tumor spheroids. Sepharose 130-137 tumor necrosis factor Homo sapiens 84-93 34082094-6 2021 Our approach"s versatility was validated by preloading tumor necrotic factor-alpha (TNF-alpha) into the composite hydrogel (MXene@agarose/TNF-alpha) to promote the pro-apoptotic signaling pathway, achieving the NIR light-induced programmed cell deaths (PCD) of tumor spheroids. Sepharose 130-137 tumor necrosis factor Homo sapiens 138-147 34146213-3 2021 Here we delineate detailed mechanism of opposing effects of CSL by confirming the binding of CSL and anti TLR 2 and 4 antibodies to TLRs 2 and 4 purified from HCECs using Galectin-3 Sepharose 4B column. Sepharose 182-191 toll like receptor 2 Homo sapiens 132-144 34345216-4 2021 Methods: The binding and modification of IGF2BP2 and SUMO1 were identified using Ni2+-NTA agarose bead pull-down assays, CO-IP and western blot; and in vitro SUMOylation assays combined with immunoprecipitation and immunofluorescence staining were performed to explore the detail affects and regulations of the SUMOylation on IGF2BP2. Sepharose 90-97 insulin like growth factor 2 mRNA binding protein 2 Homo sapiens 41-48 34345216-4 2021 Methods: The binding and modification of IGF2BP2 and SUMO1 were identified using Ni2+-NTA agarose bead pull-down assays, CO-IP and western blot; and in vitro SUMOylation assays combined with immunoprecipitation and immunofluorescence staining were performed to explore the detail affects and regulations of the SUMOylation on IGF2BP2. Sepharose 90-97 small ubiquitin-like modifier 1 Mus musculus 53-58 34221965-4 2021 A streptavidin agarose affinity assay was used to verify the direct interaction between P7C3 and phosphoglycerate kinase 1 (PGK1). Sepharose 15-22 phosphoglycerate kinase 1 Homo sapiens 124-128 34222007-5 2021 Meanwhile, the accuracy of HCP5 was verified by agarose gel electrophoresis (AGE) and Sanger sequencing. Sepharose 48-55 HLA complex P5 Homo sapiens 27-31 34157252-4 2021 Genotyping of VEGF rs35569394 polymorphism was carried out via polymerase chain reaction followed by agarose gel electrophoresis. Sepharose 101-108 vascular endothelial growth factor A Homo sapiens 14-18 34158860-4 2021 The Ni2+-NTA agarose affinity pull-down assay was carried out to determine the SUMOylation level of ANXA1. Sepharose 13-20 annexin A1 Mus musculus 100-105 34169869-6 2021 The eNOS (Glu298Asp) polymorphism was identified in EDTA blood by PCR amplification of the target region followed by restriction enzyme digestion, and genotyping on Agarose gel. Sepharose 165-172 nitric oxide synthase 3 Homo sapiens 4-8 34105490-3 2021 The cleavage products of vWF polymer by wild-type or mutant ADAMTS13 under denaturing condition or shear stress were separated by 1% SeaKem HGT agarose gel and detected by Western blot. Sepharose 144-151 von Willebrand factor Homo sapiens 25-28 34105490-3 2021 The cleavage products of vWF polymer by wild-type or mutant ADAMTS13 under denaturing condition or shear stress were separated by 1% SeaKem HGT agarose gel and detected by Western blot. Sepharose 144-151 ADAM metallopeptidase with thrombospondin type 1 motif 13 Homo sapiens 60-68 34825129-2 2021 We have previously developed Amytrap peptide (the active pharmacological ingredient, API) and linked it to a sepharose bead matrix by click chemistry to form Amytrapper matrix, which was able to bind and remove Abeta from human sera and plasma spiked with biotinylated Abeta42 (bio-Abeta42) in vitro. Sepharose 109-118 amyloid beta precursor protein Homo sapiens 211-216 34825129-7 2021 Thus, our device Amytrapper, either in the form of Sepharose matrix or catheter, could become a novel therapeutic strategy to remove Abeta from circulation in AD patients. Sepharose 51-60 amyloid beta precursor protein Homo sapiens 133-138 34780727-5 2021 For LPL purification, we used heparin-Sepharose affinity chromatography, which disrupted LPL-GPIHBP1 complexes causing GPIHBP1 to elute with the flow-through of the conditioned media. Sepharose 38-47 lipoprotein lipase Homo sapiens 89-92 34780727-5 2021 For LPL purification, we used heparin-Sepharose affinity chromatography, which disrupted LPL-GPIHBP1 complexes causing GPIHBP1 to elute with the flow-through of the conditioned media. Sepharose 38-47 glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1 Homo sapiens 93-100 34780727-5 2021 For LPL purification, we used heparin-Sepharose affinity chromatography, which disrupted LPL-GPIHBP1 complexes causing GPIHBP1 to elute with the flow-through of the conditioned media. Sepharose 38-47 glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1 Homo sapiens 119-126 35621033-4 2022 METHODS: The fusion proteins CD19-SA was expressed in CHO cells and purified by use of Ni-nitrilotriacetic acid agarose beads. Sepharose 112-119 B-lymphocyte antigen CD19 Cricetulus griseus 29-33 35572725-9 2022 We detected the tPA biological activity in vitro by fibrin agarose plate assay (FAPA). Sepharose 59-66 chromosome 20 open reading frame 181 Homo sapiens 16-19 35548965-3 2022 Nevertheless, type 1 CALR mutations are detectable by using polymerase chain reaction (PCR) and agarose gel electrophoresis. Sepharose 96-103 calreticulin Homo sapiens 21-25 35491445-7 2022 VWF multimer analysis was performed using SDS-agarose electrophoresis. Sepharose 46-53 von Willebrand factor Homo sapiens 0-3 35595154-0 2022 A comprehensive Triple Repeat Primed PCR (TR-PCR) and a Long-Range PCR (LR-PCR) agarose-based assay for improved genotyping of GAA repeats in Friedreich"s Ataxia. Sepharose 80-87 alpha glucosidase Homo sapiens 127-130 35271257-6 2022 As a demonstration, we showed that ACE I/D could be genotyped using a low amount of DNA sample (25 ng) with an accuracy of 100%, without complicated operation steps and data analysis, which is better than that of the conventional method (agarose gel electrophoresis analysis after common PCR). Sepharose 238-245 angiotensin I converting enzyme Homo sapiens 35-38 35503584-5 2022 His-BRCC3 plasmid was constructed by cloning the BRCC3 gene into pGEX-6P-1 vector, and then His-BRCC3 fusion protein was induced with isopropyl beta-d-1-thiogalactopyranoside and purified using His-Tag affinity chromatography purification agarose. Sepharose 239-246 BRCA1/BRCA2-containing complex subunit 3 Homo sapiens 4-9 35503584-5 2022 His-BRCC3 plasmid was constructed by cloning the BRCC3 gene into pGEX-6P-1 vector, and then His-BRCC3 fusion protein was induced with isopropyl beta-d-1-thiogalactopyranoside and purified using His-Tag affinity chromatography purification agarose. Sepharose 239-246 BRCA1/BRCA2-containing complex subunit 3 Homo sapiens 49-54 35503584-5 2022 His-BRCC3 plasmid was constructed by cloning the BRCC3 gene into pGEX-6P-1 vector, and then His-BRCC3 fusion protein was induced with isopropyl beta-d-1-thiogalactopyranoside and purified using His-Tag affinity chromatography purification agarose. Sepharose 239-246 BRCA1/BRCA2-containing complex subunit 3 Homo sapiens 96-101 35486294-4 2022 PROCEDURES: A time-stable material mimicking the MR properties of methemoglobin in IPH was created by doping agarose hydrogel with gadolinium and sodium alginate. Sepharose 109-116 hemoglobin subunit gamma 2 Homo sapiens 66-79 35395139-2 2022 PON1 was purified from human serum blood by ammonium sulfate precipitation (60%-80%) and hydrophobic interaction chromatography (Sepharose-4B~L-tyrosine~1-napthylamine gel). Sepharose 129-138 paraoxonase 1 Homo sapiens 0-4 35377359-6 2022 Total cellular Flag-tagged p53 protein was purified with anti-Flag antibody-conjugated agarose under nondenaturing conditions. Sepharose 87-94 tumor protein p53 Homo sapiens 27-30 35350183-0 2022 Investigation of Lysozyme Diffusion in Agarose Hydrogels Employing a Microfluidics-Based UV Imaging Approach. Sepharose 39-46 lysozyme Homo sapiens 17-25 35437514-4 2022 Nanobody-mediated capture of SARS-CoV-2-Spike (S1) on agarose beads served as the trap for soluble recombinant ACE2-GFPSpark, inhibited by neutralizing antibody. Sepharose 54-61 surface glycoprotein Severe acute respiratory syndrome coronavirus 2 40-45 35437514-4 2022 Nanobody-mediated capture of SARS-CoV-2-Spike (S1) on agarose beads served as the trap for soluble recombinant ACE2-GFPSpark, inhibited by neutralizing antibody. Sepharose 54-61 angiotensin converting enzyme 2 Homo sapiens 111-115 35350183-8 2022 The estimated diffusion coefficients for lysozyme were between 0.80 +- 0.04x10-10 m2 s-1 for 1.5% (w/w) low-melt agarose and 1.14 +- 0.02x10-10 m2 s-1 for 0.5% (w/w) unmodified agarose. Sepharose 113-120 lysozyme Homo sapiens 41-49 35350183-8 2022 The estimated diffusion coefficients for lysozyme were between 0.80 +- 0.04x10-10 m2 s-1 for 1.5% (w/w) low-melt agarose and 1.14 +- 0.02x10-10 m2 s-1 for 0.5% (w/w) unmodified agarose. Sepharose 177-184 lysozyme Homo sapiens 41-49 35249015-3 2022 The dimethylarginine dimethylaminohydrolase (DDAH) was immobilized on agarose beads to prepare a cartridge. Sepharose 70-77 dimethylarginine dimethylaminohydrolase 1 Homo sapiens 45-49 35133130-3 2022 As evidenced by agarose gel electrophoresis and liquid chromatography-mass spectrometry, it could realize the fluorescent labeling of human serum albumin (HSA) through a thiol-cyanimide addition. Sepharose 16-23 albumin Homo sapiens 140-153 35163752-8 2022 The presented data demonstrate the phosphorylation of eIF4G1 at Ser1147, Ser1185, and Ser1231 in both brain regions and in control and ischemic conditions, being the phosphorylation of eIF4G1 at Ser1147 the only one found in the eIF4E/eIF4G association complex from the cap-containing matrix (m7GTP-Sepharose). Sepharose 299-308 eukaryotic translation initiation factor 4 gamma 1 Homo sapiens 54-60 35163752-8 2022 The presented data demonstrate the phosphorylation of eIF4G1 at Ser1147, Ser1185, and Ser1231 in both brain regions and in control and ischemic conditions, being the phosphorylation of eIF4G1 at Ser1147 the only one found in the eIF4E/eIF4G association complex from the cap-containing matrix (m7GTP-Sepharose). Sepharose 299-308 eukaryotic translation initiation factor 4 gamma 1 Homo sapiens 185-191 35068137-6 2022 Taking advantage of the excellent dual pH-/photo-responsive color switching properties, we demonstrated the potential applications of the TiO2-x nanoparticles/NR/agarose gel film in dynamic rewritable paper, in which the created patterns by photo-printing produce dynamic color changing upon applying an acidic or a basic vapor. Sepharose 162-169 phenylalanine hydroxylase Homo sapiens 39-41 35141351-5 2022 Samples that screen positive for transduction inhibitors are subsequently depleted of all classes of immunoglobulins using agarose resins conjugated with protein A, G, and L (AGL), which restores AAV5 transduction for NAb-containing samples. Sepharose 123-130 amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Homo sapiens 175-178 35141351-6 2022 Unconjugated agarose resin serves as a mock control for non-specific depletion effects and facilitates normalization of the transduction efficiencies between an AGL- and mock-treated sample; the normalized value is termed the AGL/mock ratio. Sepharose 13-20 amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Homo sapiens 161-164 35141351-6 2022 Unconjugated agarose resin serves as a mock control for non-specific depletion effects and facilitates normalization of the transduction efficiencies between an AGL- and mock-treated sample; the normalized value is termed the AGL/mock ratio. Sepharose 13-20 amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase Homo sapiens 226-229 35071747-3 2022 In this study, we identified X-ray repair cross-complementing 5 (XRCC5), a novel hTERT promoter-binding protein in HCC cells, using biotin-streptavidin-agarose pull-down assay. Sepharose 152-159 X-ray repair cross complementing 5 Homo sapiens 29-63 35152379-6 2022 In this chapter, we provide a detailed protocol to assess the ubiquitination of PP2CA, a clade A PP2C, mediated by BPMs using agarose-immobilised p62-derived ubiquitin-associated (UBA) domain, which efficiently binds ubiquitinated proteins. Sepharose 126-133 protein phosphatase, Mg2+/Mn2+ dependent 1A Homo sapiens 80-85 35152379-6 2022 In this chapter, we provide a detailed protocol to assess the ubiquitination of PP2CA, a clade A PP2C, mediated by BPMs using agarose-immobilised p62-derived ubiquitin-associated (UBA) domain, which efficiently binds ubiquitinated proteins. Sepharose 126-133 nucleoporin 62 Homo sapiens 146-149 35071747-3 2022 In this study, we identified X-ray repair cross-complementing 5 (XRCC5), a novel hTERT promoter-binding protein in HCC cells, using biotin-streptavidin-agarose pull-down assay. Sepharose 152-159 X-ray repair cross complementing 5 Homo sapiens 65-70 35071747-3 2022 In this study, we identified X-ray repair cross-complementing 5 (XRCC5), a novel hTERT promoter-binding protein in HCC cells, using biotin-streptavidin-agarose pull-down assay. Sepharose 152-159 telomerase reverse transcriptase Homo sapiens 81-86 2506947-10 1989 Sodium dodecyl sulfate (SDS) 1.4% agarose gel electrophoresis showed that all multimers of vWF were present in both patients. Sepharose 34-41 von Willebrand factor Homo sapiens 91-94 2514095-17 1989 The non-glycosylated receptor from tunicamycin-treated cells appears to bind transferrin as demonstrated by interaction with transferrin-Sepharose. Sepharose 137-146 transferrin Mus musculus 77-88 2514095-17 1989 The non-glycosylated receptor from tunicamycin-treated cells appears to bind transferrin as demonstrated by interaction with transferrin-Sepharose. Sepharose 137-146 transferrin Mus musculus 125-136 2597157-2 1989 CRF-BP-CRF complex adsorbed to concanavalin-A-Sepharose and its Mr decreased after treatment with endoglycosidase H or glycopeptidase A. Sepharose 46-55 corticotropin releasing hormone binding protein Homo sapiens 0-6 2480379-3 1989 The T cell hybridomas 231F1 and 12H5 constitutively secrete glycosylation-inhibiting factor (GIF) and glycosylation-enhancing factor (GEF), respectively, which lack affinity for OVA-coupled Sepharose. Sepharose 190-199 cobalamin binding intrinsic factor Homo sapiens 93-96 2480379-3 1989 The T cell hybridomas 231F1 and 12H5 constitutively secrete glycosylation-inhibiting factor (GIF) and glycosylation-enhancing factor (GEF), respectively, which lack affinity for OVA-coupled Sepharose. Sepharose 190-199 Rho/Rac guanine nucleotide exchange factor 2 Homo sapiens 134-137 2613269-4 1989 After coupling to Sepharose 4B, RAM11 provided a useful affinity chromatography support for the purification of murine IgM antibodies from biological fluids. Sepharose 18-30 immunoglobulin heavy constant mu Mus musculus 119-122 2633960-4 1989 CRP-positive serum from Cd-treated rats precipitated with normal rat serum on agarose-covered slides in the presence of 1 mM CaCl2. Sepharose 78-85 C-reactive protein Rattus norvegicus 0-3 2576426-5 1989 Finally, P-glycoprotein-MRK 16-protein A-Sepharose complex derived from human adrenal possessed marked ATPase activity. Sepharose 41-50 ATP binding cassette subfamily B member 1 Homo sapiens 9-23 2480121-1 1989 alpha 2-Macroglobulin binds to insoluble trypsin bound on agarose beads inducing a reduction of proteolytic activity of the enzyme towards large substrates such as azocasein. Sepharose 58-65 alpha-2-macroglobulin Homo sapiens 0-21 2556811-1 1989 Protein C inhibitor (PCI) was purified from human plasma using immunoaffinity chromatography and heparin Sepharose chromatography, a method that allowed the purification of active and inactive inhibitor. Sepharose 105-114 serpin family A member 5 Homo sapiens 0-25 2609289-8 1989 The abnormal ATIII was also found to elute from heparin agarose at a lower ionic strength than normal ATIII. Sepharose 56-63 serpin family C member 1 Homo sapiens 13-18 2808426-7 1989 Combined with column chromatography of the solubilized EGF receptor on protamine-agarose gel, these results suggest that protamine may increase the EGF receptor number by directly activating cryptic EGF receptors in the plasma membrane. Sepharose 81-88 epidermal growth factor Homo sapiens 55-58 2553707-4 1989 Chromatography on DEAE-cellulose, S-Sepharose, heptyl-Sepharose, heparin-agarose, and Mono Q results in greater than 20,000-fold purification of the insulin-stimulated enzyme with a 12% recovery. Sepharose 36-45 insulin Oryctolagus cuniculus 149-156 2553707-4 1989 Chromatography on DEAE-cellulose, S-Sepharose, heptyl-Sepharose, heparin-agarose, and Mono Q results in greater than 20,000-fold purification of the insulin-stimulated enzyme with a 12% recovery. Sepharose 73-80 insulin Oryctolagus cuniculus 149-156 2553709-7 1989 The chromatographic properties of the control (dark) and photolabeled nuclear Ah receptor complexes were comparable using Sephacryl S-300 and DNA-Sepharose columns. Sepharose 146-155 aryl-hydrocarbon receptor Mus musculus 78-89 2553717-2 1989 Chromatography of solubilized membrane proteins on wheat germ agglutininagarose and aFGF-Sepharose yielded three major polypeptides migrating at 150, 70, and 45 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 89-98 fibroblast growth factor 1 Gallus gallus 84-88 2553717-3 1989 These polypeptides were eluted from aFGF-Sepharose with either 1.0 M NaCl or 100 micrograms/ml heparin, but were not retained on underivatized Sepharose. Sepharose 41-50 fibroblast growth factor 1 Gallus gallus 36-40 2572596-5 1989 Furthermore, we have prepared a monoclonal antibody against a chromatographically enriched soluble receptor fraction and used this antibody (30F3) to immunopurify the receptor in conjunction with Sepharose-somatostatin-14 immunopurification and steric exclusion high pressure liquid chromatography (HPLC). Sepharose 196-205 somatostatin Homo sapiens 206-221 2619234-1 1989 The AST responsible for a raised enzyme activity, with otherwise normal biochemistry, in a single individual over a 16-month period has been characterized as an IgG (lambda)-AST complex by (i) estimation of the molecular size by gel exclusion chromatography on Sephadex G200, (ii) agarose gel electrophoresis, (iii) immunoprecipitation by anti-immunoglobulin antisera and (iv) adsorption on to protein A-agarose. Sepharose 281-288 solute carrier family 17 member 5 Homo sapiens 4-7 2619234-1 1989 The AST responsible for a raised enzyme activity, with otherwise normal biochemistry, in a single individual over a 16-month period has been characterized as an IgG (lambda)-AST complex by (i) estimation of the molecular size by gel exclusion chromatography on Sephadex G200, (ii) agarose gel electrophoresis, (iii) immunoprecipitation by anti-immunoglobulin antisera and (iv) adsorption on to protein A-agarose. Sepharose 281-288 solute carrier family 17 member 5 Homo sapiens 174-177 2619234-1 1989 The AST responsible for a raised enzyme activity, with otherwise normal biochemistry, in a single individual over a 16-month period has been characterized as an IgG (lambda)-AST complex by (i) estimation of the molecular size by gel exclusion chromatography on Sephadex G200, (ii) agarose gel electrophoresis, (iii) immunoprecipitation by anti-immunoglobulin antisera and (iv) adsorption on to protein A-agarose. Sepharose 404-411 solute carrier family 17 member 5 Homo sapiens 4-7 2619234-1 1989 The AST responsible for a raised enzyme activity, with otherwise normal biochemistry, in a single individual over a 16-month period has been characterized as an IgG (lambda)-AST complex by (i) estimation of the molecular size by gel exclusion chromatography on Sephadex G200, (ii) agarose gel electrophoresis, (iii) immunoprecipitation by anti-immunoglobulin antisera and (iv) adsorption on to protein A-agarose. Sepharose 404-411 solute carrier family 17 member 5 Homo sapiens 174-177 2480780-3 1989 The highly purified receptors (prepared by insulin-Sepharose chromatography) were 5-10 times more effective in catalysing the phosphorylation of calmodulin than an equal number of partially purified receptors (prepared by wheat-germ agglutinin-Sepharose chromatography). Sepharose 51-60 insulin Homo sapiens 43-50 2480780-3 1989 The highly purified receptors (prepared by insulin-Sepharose chromatography) were 5-10 times more effective in catalysing the phosphorylation of calmodulin than an equal number of partially purified receptors (prepared by wheat-germ agglutinin-Sepharose chromatography). Sepharose 51-60 calmodulin 1 Homo sapiens 145-155 2480780-3 1989 The highly purified receptors (prepared by insulin-Sepharose chromatography) were 5-10 times more effective in catalysing the phosphorylation of calmodulin than an equal number of partially purified receptors (prepared by wheat-germ agglutinin-Sepharose chromatography). Sepharose 244-253 calmodulin 1 Homo sapiens 145-155 2627551-1 1989 Affinity chromatography on heparin-Sepharose was used to isolate two forms of antithrombin III(AT) from human, bovine, rabbit and rat blood plasma. Sepharose 35-44 serpin family C member 1 Homo sapiens 78-94 2612882-4 1989 The latter antibody, covalently coupled to protein A-Sepharose through the Fc region, served as an affinity matrix to purify PBP 2". Sepharose 53-62 AT695_RS11765 Staphylococcus aureus 125-128 2529352-6 1989 The two-step chromatography using 8-MeO-N-PBAT-Affigel 202 followed by wheat germ agglutinin-agarose gave a fraction enriched (by at least 400-fold) in 5-HT1A sites. Sepharose 93-100 5-hydroxytryptamine receptor 1A Rattus norvegicus 152-158 2555458-6 1989 CO was purified from detergent extracts of the mitochondria by cytochrome c-Sepharose 4B affinity chromatography. Sepharose 76-85 LOC104968582 Bos taurus 63-75 2813400-5 1989 A phosphorylated cap-binding protein was substantially enriched from lysates of control or TNF-alpha-treated ME-180 cells by affinity chromatography with 7-methylguanosine 5"-triphosphate-Sepharose. Sepharose 188-197 tumor necrosis factor Homo sapiens 91-100 2534245-1 1989 Plasmin, immobilized on Sepharose, was used for isolation of human blood plasma fibronectin fragments obtained after proteolysis. Sepharose 24-33 plasminogen Homo sapiens 0-7 2534245-1 1989 Plasmin, immobilized on Sepharose, was used for isolation of human blood plasma fibronectin fragments obtained after proteolysis. Sepharose 24-33 fibronectin 1 Homo sapiens 80-91 2534245-2 1989 Under definite conditions the major part of the fibronectin fragments, liberated during proteolysis, remained to be bound to plasmin-Sepharose. Sepharose 133-142 fibronectin 1 Homo sapiens 48-59 2534245-2 1989 Under definite conditions the major part of the fibronectin fragments, liberated during proteolysis, remained to be bound to plasmin-Sepharose. Sepharose 133-142 plasminogen Homo sapiens 125-132 2534245-3 1989 As shown by electrophoretic analysis, the fraction of fragments bound to plasmin-Sepharose constituted mainly "heavy" (greater than or equal to 120 kD) peptides and one "light" (29 kD) peptide, while only "light" fragments (less than or equal to 45 kD) were detected in the free unbound fraction. Sepharose 81-90 plasminogen Homo sapiens 73-80 2534245-4 1989 These unbound to plasmin-Sepharose fibronectin fragments were found to stimulate proliferation of human embryonal fibroblasts in cell culture, whereas the plasmin-Sepharose bound peptides did not exhibit any effects on proliferation. Sepharose 25-34 plasminogen Homo sapiens 17-24 2534245-4 1989 These unbound to plasmin-Sepharose fibronectin fragments were found to stimulate proliferation of human embryonal fibroblasts in cell culture, whereas the plasmin-Sepharose bound peptides did not exhibit any effects on proliferation. Sepharose 25-34 fibronectin 1 Homo sapiens 35-46 2534245-4 1989 These unbound to plasmin-Sepharose fibronectin fragments were found to stimulate proliferation of human embryonal fibroblasts in cell culture, whereas the plasmin-Sepharose bound peptides did not exhibit any effects on proliferation. Sepharose 163-172 plasminogen Homo sapiens 155-162 2605215-2 1989 Very high selectivity for the estrogen receptor is achieved through the use of DNA-Sepharose containing eight tandem copies of a consensus estrogen response element (ERE) DNA sequence. Sepharose 83-92 estrogen receptor 1 Homo sapiens 30-47 2620450-4 1989 In the system, a bacterial luciferase and NAD(P)H:FMN oxidoreductase are covalently co-immobilized on Sepharose 4B. Sepharose 102-114 formin 1 Homo sapiens 50-53 2680606-2 1989 The heat-stable, antibody-positive bacterial product was isolated using a papain-Sepharose affinity column and was shown to inhibit two cysteine proteinases, papain and human cathepsin B. Sepharose 81-90 cathepsin B Homo sapiens 175-186 2478127-3 1989 The binding of hsp47 to gelatin-Sepharose 4B was competitively inhibited by fetuin as effectively as by gelatin or collagen, whereas a variety of other proteins tested had no effect. Sepharose 32-41 serpin family H member 1 Homo sapiens 15-20 2478127-4 1989 Fetuin-coupled Sepharose 4B was found to bind hsp47 even at high ionic strength, but the complex was dissociated at pH less than or equal to 5.5.) Sepharose 15-24 serpin family H member 1 Homo sapiens 46-51 2507535-3 1989 This 21-kDa GBF binds repeatedly to gelatin-Sepharose, eluting near 2.6 M in a urea gradient. Sepharose 44-53 Kruppel like factor 6 Homo sapiens 12-15 2803750-0 1989 Purification of human C-reactive protein by barium sulfate and preparative agarose electrophoresis. Sepharose 75-82 C-reactive protein Homo sapiens 22-40 2803750-2 1989 The methods described take advantage of the barium sulfate adsorption property of CRP and the unique biophysical property of CRP migration during electrophoresis in agarose gels containing Ca2+. Sepharose 165-172 C-reactive protein Homo sapiens 125-128 2791274-0 1989 Apolipoprotein E phenotype determined by agarose gel electrofocusing and immunoblotting. Sepharose 41-48 apolipoprotein E Homo sapiens 0-16 2612468-0 1989 Simultaneous phenotyping of erythrocyte acid phosphatase and esterase D by nonequilibrium agarose isoelectric focusing. Sepharose 90-97 esterase D Homo sapiens 61-71 2612468-1 1989 Nonequilibrium agarose isoelectric focusing in a pH 4.5-7 gradient with 3-(N-morpholinopropanesulfonic acid (MOPS) and taurine as chemical separators presents a fast, easy and reliable method for the simultaneous determination of the common erythrocyte acid phosphatase and esterase D phenotypes in hemolysates and dried bloodstains. Sepharose 15-22 esterase D Homo sapiens 274-284 2511018-3 1989 The patient"s LpL purified from the PHP by heparin-Sepharose and phenyl-Sepharose chromatographies hydrolysed tributryrin, but not triolein emulsified with Triton X-100 and phosphatidylcholine (PC), or in chylomicrons, whereas normal LpL hydrolysed these substrates. Sepharose 51-60 lipoprotein lipase Homo sapiens 14-17 2531089-3 1989 The surface-exposed, IgE-binding alpha subunits of Fc epsilon RI [Fc epsilon RI alpha] were isolated by affinity chromatography using IgE and anti-IgE-Sepharose. Sepharose 151-160 Fc epsilon receptor Ia Rattus norvegicus 66-85 2531089-7 1989 Surprisingly and in contrast to acid-treated PMC, upon anti-IgE-Sepharose isolation acid-treated IMMC yielded an intensely radioactive Fc epsilon RI alpha band in the absence of added IgE. Sepharose 64-73 Fc epsilon receptor Ia Rattus norvegicus 135-154 2605266-0 1989 Rapid purification of porcine colostral whey lactoferrin by affinity chromatography on single-stranded DNA-agarose. Sepharose 107-114 lactotransferrin Bos taurus 45-56 2605266-3 1989 This lactoferrin was purified to homogeneity in one chromatographic step using immobilized single-stranded DNA-agarose. Sepharose 111-118 lactotransferrin Bos taurus 5-16 2592377-6 1989 (a) TSP immobilized on plastic or Sepharose bound 3-10-fold more GPIIb-IIIa than immobilized bovine serum albumin. Sepharose 34-43 integrin subunit alpha 2b Homo sapiens 65-70 2689170-6 1989 The lysyl-Sepharose step separated the enzyme from the majority of the contaminating proteins, including a 55-kDa protein which was further purified by PP-Sepharose chromatography and identified as an additional form of the 36-kDa and 34-kDa procollagen C-proteinase enhancer proteins described before [Adar et al. Sepharose 10-19 membrane protein, palmitoylated Mus musculus 107-113 2689170-6 1989 The lysyl-Sepharose step separated the enzyme from the majority of the contaminating proteins, including a 55-kDa protein which was further purified by PP-Sepharose chromatography and identified as an additional form of the 36-kDa and 34-kDa procollagen C-proteinase enhancer proteins described before [Adar et al. Sepharose 10-19 replication factor C (activator 1) 5 Mus musculus 224-230 2689170-6 1989 The lysyl-Sepharose step separated the enzyme from the majority of the contaminating proteins, including a 55-kDa protein which was further purified by PP-Sepharose chromatography and identified as an additional form of the 36-kDa and 34-kDa procollagen C-proteinase enhancer proteins described before [Adar et al. Sepharose 10-19 solute carrier family 25 (mitochondrial carrier, peroxisomal membrane protein), member 17 Mus musculus 235-241 2689170-6 1989 The lysyl-Sepharose step separated the enzyme from the majority of the contaminating proteins, including a 55-kDa protein which was further purified by PP-Sepharose chromatography and identified as an additional form of the 36-kDa and 34-kDa procollagen C-proteinase enhancer proteins described before [Adar et al. Sepharose 10-19 bone morphogenetic protein 1 Mus musculus 242-266 2689170-6 1989 The lysyl-Sepharose step separated the enzyme from the majority of the contaminating proteins, including a 55-kDa protein which was further purified by PP-Sepharose chromatography and identified as an additional form of the 36-kDa and 34-kDa procollagen C-proteinase enhancer proteins described before [Adar et al. Sepharose 155-164 membrane protein, palmitoylated Mus musculus 107-113 2689170-6 1989 The lysyl-Sepharose step separated the enzyme from the majority of the contaminating proteins, including a 55-kDa protein which was further purified by PP-Sepharose chromatography and identified as an additional form of the 36-kDa and 34-kDa procollagen C-proteinase enhancer proteins described before [Adar et al. Sepharose 155-164 replication factor C (activator 1) 5 Mus musculus 224-230 2689170-11 1989 In the course of PP-Sepharose chromatography, a large proportion of the 55-kDa protein disappeared with the concomitant appearance of the smaller enhancer proteins. Sepharose 20-29 membrane protein, palmitoylated Mus musculus 72-78 2591027-2 1989 GSTs were purified from normal colon mucosa and from colonic tumours by affinity chromatography on glutathione-agarose. Sepharose 111-118 glutathione S-transferase kappa 1 Homo sapiens 0-4 2692853-2 1989 This protein (RBP1) has been partially purified by means of heparin-agarose chromatography and protects 20 bp in the rDNA enhancer, and 25 bp in the initiation region, against DNase I in an in vitro footprinting assay. Sepharose 68-75 Sgn1p Saccharomyces cerevisiae S288C 14-18 2506947-13 1989 The qualitative abnormality of vWF in both patients was associated with a subtle alteration of the multimeric structure by SDS 3% agarose gel electrophoresis in which the two central subbands of the quintuplet of individual oligomers were undetectable or poorly visible. Sepharose 130-137 von Willebrand factor Homo sapiens 31-34 2549192-6 1989 The protein tyrosyl kinase activity of IGF-I receptors from bovine adrenal medulla and PC12 cells was examined after purification of the receptors by wheat germ agglutinin-Sepharose chromatography. Sepharose 172-181 IGFI Bos taurus 39-44 2777898-3 1989 Whereas bFGF and aFGF bind tightly to heparin and elute from a heparin-Sepharose column with 2 M NaCl and 1.6 M NaCl, respectively, TGFe binds to heparin with lower affinity and can be eluted from heparin-Sepharose column with 0.5 M NaCl. Sepharose 71-80 fibroblast growth factor 2 Homo sapiens 8-12 2777898-3 1989 Whereas bFGF and aFGF bind tightly to heparin and elute from a heparin-Sepharose column with 2 M NaCl and 1.6 M NaCl, respectively, TGFe binds to heparin with lower affinity and can be eluted from heparin-Sepharose column with 0.5 M NaCl. Sepharose 71-80 fibroblast growth factor 1 Homo sapiens 17-21 2482285-5 1989 Affinity chromatography using Ricinus communis agglutinin-conjugated Sepharose showed that the serum hCG was completely sialylated, while the urinary hCG was partially desialylated. Sepharose 69-78 chorionic gonadotropin subunit beta 5 Homo sapiens 101-104 2534619-1 1989 Electrophoretic purified fibronectin (FN) was isolated with high concentration (0.5 mg/ml plasma) from porcine plasma by affinity chromatography with gelatin-sepharose 4B and heparin-sepharose 4B. Sepharose 158-167 fibronectin 1 Homo sapiens 25-36 2769267-2 1989 Brain TTN was purified by immunoaffinity chromatography with polyclonal octadecaneuropeptide, DBI 33-50) antibodies coupled to CNBr-Sepharose 4B followed by two reverse-phase HPLC steps. Sepharose 132-141 diazepam binding inhibitor Rattus norvegicus 6-9 2682628-2 1989 P71 was copurified by ATP-agarose affinity chromatography with three additional proteins of the Hsp70 family. Sepharose 26-33 zinc finger protein 398 Homo sapiens 0-3 2534619-1 1989 Electrophoretic purified fibronectin (FN) was isolated with high concentration (0.5 mg/ml plasma) from porcine plasma by affinity chromatography with gelatin-sepharose 4B and heparin-sepharose 4B. Sepharose 158-167 fibronectin 1 Homo sapiens 38-40 2534619-1 1989 Electrophoretic purified fibronectin (FN) was isolated with high concentration (0.5 mg/ml plasma) from porcine plasma by affinity chromatography with gelatin-sepharose 4B and heparin-sepharose 4B. Sepharose 183-192 fibronectin 1 Homo sapiens 25-36 2534619-2 1989 The isolated FN shows one single band (MW 450,000) both in agarose gel and PAGE, and two similar bands (MW 230,000) in the presence of 1% beta-mercaptoethanol in SDS-PAGE. Sepharose 59-66 fibronectin 1 Homo sapiens 13-15 2552660-6 1989 The 31-kDa D12L protein copurified with the virus capping enzyme through chromatography on heparin-agarose and phosphocellulose and also cosedimented with the capping enzyme through a glycerol density gradient. Sepharose 99-106 small subunit of mRNA capping enzyme Vaccinia virus 11-15 2481574-4 1989 For this purpose, metabolically labelled rAGP, secreted by isolated hepatocytes under the various conditions, was separated on Con A-Sepharose into four fractions. Sepharose 133-142 orosomucoid 1 Rattus norvegicus 41-45 2510350-8 1989 Analysis of plasma vWF multimer patterns by 1.4% agarose electrophoresis in 0.1% SDS revealed excessive amounts of large, medium and small size multimers in the PIH patients. Sepharose 49-56 von Willebrand factor Homo sapiens 19-22 2570779-7 1989 The kinase activity was found to co-purify with TH activity through ammonium sulfate precipitation and DEAE-cellulose chromatography and could be only partially resolved from TH by heparin-agarose affinity chromatography. Sepharose 189-196 tyrosine hydroxylase Rattus norvegicus 48-50 2677147-3 1989 The method uses agarose containing anti-human IgA or anti-human IgG heavy chain-specific reagent to which protease-digested human immunoglobulin samples are applied to wells and electrophoresed overnight. Sepharose 16-23 immunoglobulin heavy variable 4-38-2-like Homo sapiens 46-49 2798097-0 1989 Pulse time and agarose concentration affect the electrophoretic mobility of cccDNA during PFGE and FIGE [corrected]. Sepharose 15-22 sulfatase modifying factor 2 Homo sapiens 90-94 2475504-4 1989 Here we report the preparation of alpha 2M receptors from rat liver membranes solubilized in 3-[(3-cholamidopropyl) dimethylammonio]-1-propane sulfonic acid (CHAPS) dihydrate and incubated with Sepharose-immobilized alpha 2M-methylamine. Sepharose 194-203 alpha-2-macroglobulin Rattus norvegicus 34-42 2510823-2 1989 The binding of t-PA to lysine-Sepharose and aminohexyl-Sepharose was found to require kringle 2. Sepharose 30-39 plasminogen activator, tissue type Homo sapiens 15-19 2477279-3 1989 The membranes were solubilized in 3-[(3-cholamidopropyl)dimethylammonio]propane sulfonate (CHAPS) and affinity chromatography was performed using Sepharose-immobilized alpha 2-macroglobulin-methylamine with elution in buffer containing 2 mM EDTA, pH 6.0. Sepharose 146-155 alpha-2-macroglobulin Homo sapiens 168-189 2477279-8 1989 125I-iodinated receptor preparation bound almost quantitatively to Sepharose-immobilized alpha 2-macroglobulin-methylamine in the presence of CHAPS alone, and bound 70-80% in the presence of 0.2% SDS. Sepharose 67-76 alpha-2-macroglobulin Homo sapiens 89-110 2774557-0 1989 Isolation of human blood coagulation alpha-factor Xa by soybean trypsin inhibitor-sepharose chromatography and its active-site titration with fluorescein mono-p-guanidinobenzoate. Sepharose 82-91 coagulation factor X Homo sapiens 43-52 2774557-0 1989 Isolation of human blood coagulation alpha-factor Xa by soybean trypsin inhibitor-sepharose chromatography and its active-site titration with fluorescein mono-p-guanidinobenzoate. Sepharose 82-91 kunitz trypsin protease inhibitor Glycine max 64-81 2774557-1 1989 A method based on active-site affinity chromatography on soybean trypsin inhibitor (SBTI)-Sepharose was developed for isolation of human factor Xa in primarily the undergraded alpha-form. Sepharose 90-99 kunitz trypsin protease inhibitor Glycine max 65-82 2774557-1 1989 A method based on active-site affinity chromatography on soybean trypsin inhibitor (SBTI)-Sepharose was developed for isolation of human factor Xa in primarily the undergraded alpha-form. Sepharose 90-99 coagulation factor X Homo sapiens 137-146 2774557-6 1989 Characterization of the reaction of fluorescein mono-p-guanidinobenzoate (FMGB) with human and bovine factor Xa isolated by SBTI-Sepharose chromatography demonstrated its utility as a sensitive reagent for continuous fluorometric active-site titration. Sepharose 129-138 coagulation factor X Homo sapiens 102-111 2511928-1 1989 It was shown that delipidated rhodopsin immobilized on concanavalin A-Sepharose (Rimm) binds with high selectivity transducin from total extracts of rod outer segment protein as well as the regulatory GTP-binding Gi- and Go-like proteins from solubilized membranes of bovine brain. Sepharose 70-79 rhodopsin Bos taurus 30-39 2552998-3 1989 gC1 was isolated from herpes-simplex-virus-infected BHK (baby-hamster kidney) cells after short labelling periods with [3H]glucosamine, and the labelled Pronase-cleaved glycopeptides fractionated on concanavalin A-Sepharose. Sepharose 214-223 solute carrier family 25 member 22 Homo sapiens 0-3 2550475-2 1989 Oligosaccharides (tetra- to decasaccharides) obtained by nitrous acid depolymerisation of standard heparin were separated by affinity chromatography on Sepharose-immobilised aFGF. Sepharose 152-161 fibroblast growth factor 1 Homo sapiens 174-178 2817389-3 1989 We report here a procedure for the analysis of NMT activity using ion-exchange chromatography on CM-Sepharose to separate [3H]myristoyl peptide from radiolabeled reactants. Sepharose 100-109 N-myristoyltransferase 1 Homo sapiens 47-50 2768893-8 1989 Presumably W-7 would perturb any physiologically relevant CaM-protein interactions in cells but W-7 concentrations that reduced HSP and other protein binding to CaM-agarose columns by 50 per cent or more, had no effect on thermotolerance development in cells. Sepharose 165-172 calmodulin 1 Rattus norvegicus 161-164 2475570-4 1989 CD59 antigen was purified from human urine and erythrocyte stroma by affinity chromatography using the mAb YTH 53.1 immobilized on Sepharose, and, following transient expression of a human T cell cDNA library in COS cells, the corresponding cDNA also identified using the antibody. Sepharose 131-140 CD59 molecule (CD59 blood group) Homo sapiens 0-4 2575117-6 1989 In lectin affinity chromatography with concanavalin A and wheat germ lectin sepharose, serum dipeptidyl aminopeptidase IV from liver cirrhosis patients showed the same binding pattern as that from healthy subjects. Sepharose 76-85 carboxypeptidase Q Homo sapiens 104-118 2788710-6 1989 The NDR activity comigrates with BLT-esterase activity during subcellular fractionation, solubilization, gel filtration, and aprotinin-Sepharose affinity chromatography. Sepharose 135-144 serine/threonine kinase 38 Homo sapiens 4-7 2779227-5 1989 The two most prominent calmodulin-binding proteins isolated from cytosol by calmodulin-Sepharose column chromatography had Mr of 60,000 and 47,000. Sepharose 87-96 calmodulin-2 Cavia porcellus 23-33 2779227-5 1989 The two most prominent calmodulin-binding proteins isolated from cytosol by calmodulin-Sepharose column chromatography had Mr of 60,000 and 47,000. Sepharose 87-96 calmodulin-2 Cavia porcellus 76-86 2482578-1 1989 Combination of two procedures--pseudoligand affinity chromatography on Cibacron blue agarose and usual gel filtration on TSK HW-60 enabled to separate successfully blood plasma alpha 2-macroglobulin from immunoglobulin M, haptoglobins and low molecular weight proteins. Sepharose 85-92 alpha-2-macroglobulin Homo sapiens 177-198 2621691-1 1989 Two isoenzymes of oxytocinase activity were fractionated from human seminal plasma by acrylamide-agarose gel chromatography and partly characterized using S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN) separately as substrates. Sepharose 97-104 leucyl and cystinyl aminopeptidase Homo sapiens 18-29 2479295-0 1989 Separation and visualization of apolipoprotein B species by sodium dodecyl sulfate-agarose gel electrophoresis and immunoblotting. Sepharose 83-90 apolipoprotein B Homo sapiens 32-48 2479295-1 1989 A method for separation and visualization of the different apolipoprotein B species using 0.2% sodium dodecyl sulfate-1.5% agarose gel electrophoresis and immunoblotting is described. Sepharose 123-130 apolipoprotein B Homo sapiens 59-75 2814937-2 1989 The abnormal antithrombin was isolated from plasma by chromatography on heparin-Sepharose at pH 6.0, and ion exchange on DEAE-Sephadex at pH 8.6 and 6.0. Sepharose 80-89 serpin family C member 1 Homo sapiens 13-25 2527207-9 1989 Polyclonal TcR gamma/delta+ cells were obtained by direct stimulation of peripheral blood mononuclear cells with Sepharose bead-conjugated anti-TcR gamma/delta MAbs and IL-2. Sepharose 113-122 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 11-14 2527207-9 1989 Polyclonal TcR gamma/delta+ cells were obtained by direct stimulation of peripheral blood mononuclear cells with Sepharose bead-conjugated anti-TcR gamma/delta MAbs and IL-2. Sepharose 113-122 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 144-147 2571374-1 1989 OBJECTIVE: To assess the value of serum carbohydrate deficient transferrin as detected by isoelectric focusing on agarose as an indicator of alcohol abuse. Sepharose 114-121 transferrin Homo sapiens 63-74 2665860-2 1989 In reconstitution experiments, one CML cell per million normal mononuclear cells could be detected by direct agarose gel visualization of a bcr/abl-specific band following PCR. Sepharose 109-116 BCR activator of RhoGEF and GTPase Homo sapiens 140-143 2764118-4 1989 DEAE-Sephacyl ion exchange and gelatin-Sepharose affinity chromatography revealed two peaks containing chemotactic activity, one of which may be fibronectin, since it binds to gelatin, reacts in a specific immunoassay, and is inhibited of chemotactic activity by anti-fibronectin antiserum, and another of which does not appear to be fibronectin, since it does not bind to gelatin nor react in the immunoassay. Sepharose 39-48 fibronectin 1 Homo sapiens 145-156 2752139-3 1989 Purification of this recombinant human erythropoietin to virtual homogeneity (greater than or equal to 99%) was accomplished via a simple three-step procedure involving isocratic elution from DEAE-Sephacel, reverse-phase high performance liquid chromatography (HPLC) on a C4 medium, and the single-step elution of purified hormone from concanavalin A agarose. Sepharose 351-358 erythropoietin Homo sapiens 39-53 2818602-2 1989 Glyceraldehyde-3-phosphate dehydrogenase covalently bound to CNBr-activated Sepharose 4B was capable of forming a complex with soluble lactate dehydrogenase with a stoichiometry of 0.8 mole of lactate dehydrogenase per mole of glyceraldehyde-3-phosphate dehydrogenase and KD of 0.385 microM at pH 6.5. Sepharose 76-85 glyceraldehyde-3-phosphate dehydrogenase Oryctolagus cuniculus 0-40 2818602-2 1989 Glyceraldehyde-3-phosphate dehydrogenase covalently bound to CNBr-activated Sepharose 4B was capable of forming a complex with soluble lactate dehydrogenase with a stoichiometry of 0.8 mole of lactate dehydrogenase per mole of glyceraldehyde-3-phosphate dehydrogenase and KD of 0.385 microM at pH 6.5. Sepharose 76-85 glyceraldehyde-3-phosphate dehydrogenase Oryctolagus cuniculus 227-267 2758635-2 1989 CBG was purified from pooled human serum by precipitation with ammonium sulfate and successive affinity chromatography treatments on corticosterone-Sepharose and concanavalin A-Sepharose. Sepharose 148-157 serpin family A member 6 Homo sapiens 0-3 2758635-2 1989 CBG was purified from pooled human serum by precipitation with ammonium sulfate and successive affinity chromatography treatments on corticosterone-Sepharose and concanavalin A-Sepharose. Sepharose 177-186 serpin family A member 6 Homo sapiens 0-3 2806210-1 1989 Several genetic variants and also isoforms of transferrin differing in carbohydrate structure can be separated by polyacrylamide or agarose gel isoelectric focusing. Sepharose 132-139 transferrin Homo sapiens 46-57 2550492-2 1989 While all three SCLC lines expressed messenger RNA encoding pro-gastrin releasing peptide (GRP), only the NCI-H345 cells expressed detectable membrane receptors for GRP and responded to nanomolar concentrations of bombesin as shown by 125I-GRP binding, total inositol phosphate accumulation, and increased clonal growth in soft agarose. Sepharose 328-335 gastrin releasing peptide Homo sapiens 214-222 2526822-9 1989 Binding to lentil lectin-Sepharose 4B further sustained the glycoprotein nature of the CR1. Sepharose 25-34 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 87-90 2770298-2 1989 A method is described to measure the fraction of steroid bound to sex hormone binding globulin (SHBG) in serum, using binding of SHBG to Con-A-sepharose. Sepharose 143-152 sex hormone binding globulin Homo sapiens 66-94 2770298-2 1989 A method is described to measure the fraction of steroid bound to sex hormone binding globulin (SHBG) in serum, using binding of SHBG to Con-A-sepharose. Sepharose 143-152 sex hormone binding globulin Homo sapiens 96-100 2770298-2 1989 A method is described to measure the fraction of steroid bound to sex hormone binding globulin (SHBG) in serum, using binding of SHBG to Con-A-sepharose. Sepharose 143-152 sex hormone binding globulin Homo sapiens 129-133 2811869-6 1989 Using these antibodies, [3H]PAF and Protein A-Sepharose as a means of separating bound and free tracer, the feasibility of developing a quantitative RIA for PAF was demonstrated. Sepharose 46-55 PCNA clamp associated factor Homo sapiens 157-160 2585927-2 1989 Affinity chromatography using HE agarose gel was the most effective method to isolate both CRP and SAP from a large volume of bovine serum. Sepharose 33-40 C-reactive protein Bos taurus 91-94 2585927-2 1989 Affinity chromatography using HE agarose gel was the most effective method to isolate both CRP and SAP from a large volume of bovine serum. Sepharose 33-40 amyloid P component, serum Bos taurus 99-102 2585927-5 1989 Bovine CRP moved slower than beta-globulin, and bovine SAP moved in the beta-globulin region in agarose gel electrophoresis. Sepharose 96-103 amyloid P component, serum Bos taurus 55-58 2685404-3 1989 Patients" IgG that passed through an antigen column packed with TPO-coupled Sepharose 4 B beads lost their hemagglutinating activity in microsome hemagglutination test. Sepharose 76-85 thyroid peroxidase Homo sapiens 64-67 2552568-4 1989 Further fractionation of high molecular weight proteases on Con A-Sepharose revealed that, unlike collagenase, chymotrypsin- and elastase-like proteases, the trypsin-like protease was bound by the affinity column. Sepharose 66-75 kallikrein related peptidase 11 Homo sapiens 158-179 2588299-0 1989 Covalent immobilization of the estrogen receptor to an electrostatically neutral N-hydroxysuccinimide ester derivative of agarose. Sepharose 122-129 estrogen receptor 1 Homo sapiens 31-48 2551374-8 1989 Peak 1 isolated from cytosol by DNA-Sepharose and incubated with hepatic cytosol from D2 mouse (which contains no detectable receptor) transformed into peak 2, suggesting that these two forms are different conformations of the same protein. Sepharose 36-45 pseudopodium-enriched atypical kinase 1 Mus musculus 0-6 2551374-9 1989 Sucrose density gradient and gel filtration analyses of peaks 1 and 2 isolated from DNA-Sepharose indicated that (i) the untransformed form (peak 1) was smaller than the unoccupied and the transformed forms, (ii) 0.4 M KCl in the density gradients had little effect on these isolated forms, and (iii) nuclear receptor sedimented like peak 2. Sepharose 88-97 pseudopodium-enriched atypical kinase 1 Mus musculus 141-147 2583314-0 1989 [Assay of activated form of progesterone receptor with ATP-sepharose column chromatography]. Sepharose 59-68 progesterone receptor Oryctolagus cuniculus 28-49 2753164-3 1989 After purification and separation of GST on glutathione Sepharose 6 B and reversed-phase HPLC, respectively, it was observed in vitro that PB caused an increase in the relative amounts of subunits 1, 3 and 7 compared to subunits 2 and 4. Sepharose 56-65 hematopoietic prostaglandin D synthase Rattus norvegicus 37-40 2545693-5 1989 A protein which protects cells against the cytotoxicity of TNF was purified from human urine by chromatography on CM-Sepharose followed by high performance liquid chromatography on Mono Q and Mono S columns and reversed phase high performance liquid chromatography. Sepharose 117-126 tumor necrosis factor Homo sapiens 59-62 2547466-3 1989 Chromatography of media from cells from normal mice treated with PTH on lysine-Sepharose resulted in the separation of latent collagenase and latent gelatinase. Sepharose 79-88 parathyroid hormone Mus musculus 65-68 2547466-5 1989 Collagenase activity of media of cells from normal or mi/mi mice treated with PTH or LPS yielded identical elution patterns upon chromatography on lysine-Sepharose. Sepharose 154-163 parathyroid hormone Mus musculus 78-81 2777757-5 1989 Both MAP 1 and MAP 2 were bound to a PLAA-Sepharose 4B affinity column, while tubulin was not retained by the column. Sepharose 42-51 Blood pressure QTL 196 Rattus norvegicus 5-10 2777757-5 1989 Both MAP 1 and MAP 2 were bound to a PLAA-Sepharose 4B affinity column, while tubulin was not retained by the column. Sepharose 42-51 microtubule-associated protein 2 Rattus norvegicus 15-20 2549150-1 1989 We report the purification of human corticotrophin-releasing factor-binding protein (hCRF-BP) using repeated affinity chromatography (with the aid of synthetic CRF immobilized on Sepharose 4B solid phase) followed by gel filtration. Sepharose 179-188 corticotropin releasing hormone binding protein Homo sapiens 85-92 2476174-1 1989 A form of human alpha 2-macroglobulin (alpha 2M) has been prepared that has properties intermediate to those of native alpha 2-macroglobulin and 2:1 protease-alpha 2 M ternary complex by using Sepharose-linked chymotrypsin. Sepharose 193-202 alpha-2-macroglobulin Homo sapiens 16-37 2476174-1 1989 A form of human alpha 2-macroglobulin (alpha 2M) has been prepared that has properties intermediate to those of native alpha 2-macroglobulin and 2:1 protease-alpha 2 M ternary complex by using Sepharose-linked chymotrypsin. Sepharose 193-202 alpha-2-macroglobulin Homo sapiens 39-47 2775713-1 1989 The accessibility of peptide bonds to cleavage by Staphylococcus aureus V8 protease bound on a Sepharose matrix was used as a conformational probe in the study of the unfolding-folding transition of phosphoglycerate kinase induced by guanidine hydrochloride. Sepharose 95-104 AT695_RS13775 Staphylococcus aureus 199-222 2786912-0 1989 Preparation of monoclonal antibodies to C3b by immunization with C3b(i)-sepharose. Sepharose 72-81 endogenous retrovirus group K member 3 Homo sapiens 40-43 2786912-0 1989 Preparation of monoclonal antibodies to C3b by immunization with C3b(i)-sepharose. Sepharose 72-81 endogenous retrovirus group K member 3 Homo sapiens 65-68 2786912-3 1989 Instead, C3b and C3bi were deposited on Sepharose 4B via the alternative pathway of complement activation in normal human serum, and this C3b(i)-Sepharose served as the immunogen. Sepharose 40-52 endogenous retrovirus group K member 3 Homo sapiens 9-12 2786912-3 1989 Instead, C3b and C3bi were deposited on Sepharose 4B via the alternative pathway of complement activation in normal human serum, and this C3b(i)-Sepharose served as the immunogen. Sepharose 40-52 endogenous retrovirus group K member 3 Homo sapiens 17-20 2786912-3 1989 Instead, C3b and C3bi were deposited on Sepharose 4B via the alternative pathway of complement activation in normal human serum, and this C3b(i)-Sepharose served as the immunogen. Sepharose 40-49 endogenous retrovirus group K member 3 Homo sapiens 9-12 2786912-3 1989 Instead, C3b and C3bi were deposited on Sepharose 4B via the alternative pathway of complement activation in normal human serum, and this C3b(i)-Sepharose served as the immunogen. Sepharose 40-49 endogenous retrovirus group K member 3 Homo sapiens 17-20 2786912-4 1989 C3b(i)-Sepharose was also prepared from a number of primate and non-primate sources, and this allowed us to demonstrate that the anti-human C3b MAbs cross-reacted with primate-derived C3b, but not with C3b from non-primates. Sepharose 7-16 endogenous retrovirus group K member 3 Homo sapiens 0-3 2786912-4 1989 C3b(i)-Sepharose was also prepared from a number of primate and non-primate sources, and this allowed us to demonstrate that the anti-human C3b MAbs cross-reacted with primate-derived C3b, but not with C3b from non-primates. Sepharose 7-16 endogenous retrovirus group K member 3 Homo sapiens 140-143 2786912-4 1989 C3b(i)-Sepharose was also prepared from a number of primate and non-primate sources, and this allowed us to demonstrate that the anti-human C3b MAbs cross-reacted with primate-derived C3b, but not with C3b from non-primates. Sepharose 7-16 endogenous retrovirus group K member 3 Homo sapiens 140-143 2786912-4 1989 C3b(i)-Sepharose was also prepared from a number of primate and non-primate sources, and this allowed us to demonstrate that the anti-human C3b MAbs cross-reacted with primate-derived C3b, but not with C3b from non-primates. Sepharose 7-16 endogenous retrovirus group K member 3 Homo sapiens 140-143 2722864-5 1989 Accordingly, we have isolated the variant from plasma using heparin-Sepharose chromatography, followed by chromatography upon thrombin-Sepharose to remove the normal antithrombin that is present (the propositus is heterozygous for the variant). Sepharose 135-144 coagulation factor II, thrombin Homo sapiens 126-134 2736257-2 1989 The sample containing 94% of cross-linked complex and 6% of free cytochrome P-450SCC was obtained after purification on cholate-Sepharose. Sepharose 128-137 cholesterol side-chain cleavage enzyme, mitochondrial Bos taurus 65-84 2781509-2 1989 The variant antithrombin was isolated from plasma of the propositus by chromatography on heparin-Sepharose, followed by passage through thrombin-Sepharose to remove the normal antithrombin component that is present. Sepharose 97-106 serpin family C member 1 Homo sapiens 12-24 2781509-2 1989 The variant antithrombin was isolated from plasma of the propositus by chromatography on heparin-Sepharose, followed by passage through thrombin-Sepharose to remove the normal antithrombin component that is present. Sepharose 97-106 coagulation factor II, thrombin Homo sapiens 16-24 2781509-2 1989 The variant antithrombin was isolated from plasma of the propositus by chromatography on heparin-Sepharose, followed by passage through thrombin-Sepharose to remove the normal antithrombin component that is present. Sepharose 145-154 serpin family C member 1 Homo sapiens 12-24 2781509-2 1989 The variant antithrombin was isolated from plasma of the propositus by chromatography on heparin-Sepharose, followed by passage through thrombin-Sepharose to remove the normal antithrombin component that is present. Sepharose 145-154 coagulation factor II, thrombin Homo sapiens 16-24 2470757-7 1989 The following observations suggest that the structure capable of recognizing Lex per se on F9 cells is Lex: (i) Cell surface-labeled components solubilized in octylglucoside, affinity-bound on an Lex-octyl-Sepharose column, contained glycoproteins reactive with anti-Lex antibody. Sepharose 206-215 fucosyltransferase 4 Mus musculus 77-80 2470757-7 1989 The following observations suggest that the structure capable of recognizing Lex per se on F9 cells is Lex: (i) Cell surface-labeled components solubilized in octylglucoside, affinity-bound on an Lex-octyl-Sepharose column, contained glycoproteins reactive with anti-Lex antibody. Sepharose 206-215 fucosyltransferase 4 Mus musculus 103-106 2470757-7 1989 The following observations suggest that the structure capable of recognizing Lex per se on F9 cells is Lex: (i) Cell surface-labeled components solubilized in octylglucoside, affinity-bound on an Lex-octyl-Sepharose column, contained glycoproteins reactive with anti-Lex antibody. Sepharose 206-215 fucosyltransferase 4 Mus musculus 103-106 2470757-7 1989 The following observations suggest that the structure capable of recognizing Lex per se on F9 cells is Lex: (i) Cell surface-labeled components solubilized in octylglucoside, affinity-bound on an Lex-octyl-Sepharose column, contained glycoproteins reactive with anti-Lex antibody. Sepharose 206-215 fucosyltransferase 4 Mus musculus 103-106 2525052-1 1989 Compound 48/80 (48/80), a mixture of polycationic compounds was fractionated using affinity chromatography on calmodulin-Sepharose. Sepharose 121-130 calmodulin 1 Homo sapiens 110-120 2525052-7 1989 A fraction designated as Fraction III bound to calmodulin-Sepharose in the presence of Ca2+ and low salt and was eluted in the absence of Ca2+ and 0.15 M NaCl. Sepharose 58-67 calmodulin 1 Homo sapiens 47-57 2656700-3 1989 After centrifugation, the supernatants (+/- Ca2+) were applied to calmodulin-Sepharose columns. Sepharose 77-86 calmodulin 1 Rattus norvegicus 66-76 2722854-4 1989 The 85-kDa, as well as the 93-kDa, receptors bound to a monoclonal antibody raised against the transferrin receptor or to transferrin-Sepharose. Sepharose 134-143 transferrin Homo sapiens 95-106 2732474-5 1989 Since jacalin binds only a small number of human serum proteins it appeared that jacalin-agarose affinity chromatography would constitute a very selective early step for the purification of C1-INH. Sepharose 89-96 serpin family G member 1 Homo sapiens 190-196 2732474-6 1989 Consequently we have designed a new, simplified three-step procedure for the purification of C1-INH which includes PEG fractionation, jacalin-agarose chromatography and hydrophobic interaction chromatography on phenyl-Sepharose which takes advantage of the marked hydrophilicity of the inhibitor. Sepharose 142-149 serpin family G member 1 Homo sapiens 93-99 2470407-12 1989 Mammalian SSB/LDH bound to RNA as demonstrated by affinity chromatography on poly(A)-agarose and by its effect on translation of mRNA in vitro. Sepharose 85-92 small RNA binding exonuclease protection factor La Homo sapiens 10-13 2735931-3 1989 In a previous report, we demonstrated that PKC binds directly to W7 when the naphthalenesulfonamide is immobilized on agarose. Sepharose 118-125 proline rich transmembrane protein 2 Homo sapiens 43-46 2735931-4 1989 In the present report, we have defined the mechanism of the binding of PKC to W7-agarose, and its relevance to the inhibitory mechanism of the naphthalenesulfonamide. Sepharose 81-88 proline rich transmembrane protein 2 Homo sapiens 71-74 2735931-5 1989 We demonstrate that PKC bound W7-agarose through the catalytic domain of the enzyme. Sepharose 33-40 proline rich transmembrane protein 2 Homo sapiens 20-23 2735931-6 1989 An active catalytic fragment of PKC was generated by limited proteolysis, and we found that this fragment bound W7-agarose and coeluted with intact PKC upon the addition of Triton X-100. Sepharose 115-122 proline rich transmembrane protein 2 Homo sapiens 32-35 2735931-11 1989 Consistent with the kinetic evidence that W7 serves as a competitive inhibitor of PKC with respect to ATP, we found that, in the presence of 10 mM MgCl2, 1 mM ATP was sufficient to elute PKC from W7-agarose. Sepharose 199-206 proline rich transmembrane protein 2 Homo sapiens 187-190 2803227-2 1989 ALDH3 isozyme was isolated from a liver via carboxymethyl-Sephadex and blue Sepharose chromatographies and its kinetic constants for various substrates and coenzymes were determined. Sepharose 76-85 aldehyde dehydrogenase 3 family member A1 Homo sapiens 0-5 2785886-3 1989 In the present study, we showed that pulmonary granulomas were induced in mice by the intratracheal injection of agarose beads coupled to recombinant interleukin 1 (IL-1) or tumor necrosis factor-alpha (TNF-alpha). Sepharose 113-120 tumor necrosis factor Mus musculus 174-201 2606304-2 1989 Cathepsin B activity was partially purified by gel filtration on TSK G3000SW, Con-A Sepharose chromatography, Phenyl-Superose column chromatography, and Mono S column chromatography. Sepharose 84-93 cathepsin B Homo sapiens 0-11 2675969-7 1989 Gel filtration of nondenatured CP4 on 4% agarose showed a high apparent molecular mass complex comprised of disulfide-bonded trimers of the 43-kDa subunits. Sepharose 41-48 surfactant protein D Rattus norvegicus 31-34 2742875-7 1989 Whereas the starting hHDL3 ligand was free of apoE, there was a substantial (7-fold) conversion of the HDL3 to apoE-containing HDL3 following in vivo circulation of the ligand, as shown by sodium phosphotungstate-MgCl2 precipitation or heparin-Sepharose column chromatography. Sepharose 244-253 apolipoprotein E Rattus norvegicus 111-115 2742875-7 1989 Whereas the starting hHDL3 ligand was free of apoE, there was a substantial (7-fold) conversion of the HDL3 to apoE-containing HDL3 following in vivo circulation of the ligand, as shown by sodium phosphotungstate-MgCl2 precipitation or heparin-Sepharose column chromatography. Sepharose 244-253 HDL3 Homo sapiens 103-107 2502113-1 1989 We have shown that delipidated rhodopsin immobilized on Concanavalin A-Sepharose is capable of binding transducin from crude bovine rod outer segment proteins and GIP-binding proteins (G proteins) of Go/Gi-type from solubilized bovine brain membrane as well. Sepharose 71-80 rhodopsin Bos taurus 31-40 2546544-4 1989 Incubation of the immunopurified protein-A-Sepharose-adsorbed PR with the catalytic subunit of cAMP-PK in the presence of gamma-[32P]ATP and divalent cations resulted in a Mg++-dependent incorporation of 32P-radioactivity into both the 114 kDa and the hsp-90 peptides. Sepharose 43-52 progesterone receptor Bos taurus 62-64 2550068-3 1989 Two cytosolic forms (PLC-I and PLC-II) of phosphoinositide-specific phospholipase C were also resolved on a phenyl-Sepharose column. Sepharose 115-124 phospholipase C beta 1 Homo sapiens 21-26 2550068-3 1989 Two cytosolic forms (PLC-I and PLC-II) of phosphoinositide-specific phospholipase C were also resolved on a phenyl-Sepharose column. Sepharose 115-124 phospholipase C gamma 1 Homo sapiens 31-37 2500433-9 1989 Fab fragments of antibodies raised to these peptides and affinity purified on factor X-agarose columns inhibited both the purified and coagulation-based assays in a dose-dependent manner. Sepharose 87-94 FA complementation group B Homo sapiens 0-3 2624756-4 1989 Molecular sieve chromatography on Sephadex G-150 and affinity chromatography on gelatin-Sepharose revealed that there was one peak of chemotactic activity in high molecular weight range, which bound to gelatin, thus suggesting that the chemotactic factor might be fibronectin. Sepharose 88-97 fibronectin 1 Homo sapiens 264-275 2549966-10 1989 Vasopressin-binding activity was co-eluted with PI-PLC I and GTP[S]-binding activity on a DEAE-Sepharose column. Sepharose 95-104 arginine vasopressin Rattus norvegicus 0-11 2550084-8 1989 Affinity chromatography with bFGF immobilized on Sepharose 4B was then performed. Sepharose 49-61 fibroblast growth factor 2 Bos taurus 29-33 2550084-9 1989 Covalent fixation of bFGF to the Sepharose matrix was carried out in presence of N-acetylated heparin in order to protect the recognition site for bFGF on its receptor. Sepharose 33-42 fibroblast growth factor 2 Bos taurus 21-25 2550084-9 1989 Covalent fixation of bFGF to the Sepharose matrix was carried out in presence of N-acetylated heparin in order to protect the recognition site for bFGF on its receptor. Sepharose 33-42 fibroblast growth factor 2 Bos taurus 147-151 2471709-2 1989 Human TSH receptor-binding activity was purified about 1,270-fold by sequential affinity chromatography on wheat germ lectin-agarose and TSH-agarose. Sepharose 125-132 thyroid stimulating hormone receptor Homo sapiens 6-18 2471709-2 1989 Human TSH receptor-binding activity was purified about 1,270-fold by sequential affinity chromatography on wheat germ lectin-agarose and TSH-agarose. Sepharose 141-148 thyroid stimulating hormone receptor Homo sapiens 6-18 2794798-5 1989 The HSL was adsorbed to heparin-Sepharose and the CO- and PNPB-hydrolyzing activities were eluted together in the same peak. Sepharose 32-41 lipase E, hormone sensitive type Bos taurus 4-7 2674675-2 1989 The yeast transcriptional activator protein GCN4 was coupled to a Sepharose column, and binding sites were isolated by passing short, random-sequence oligonucleotides over the column and eluting them with increasing salt concentrations. Sepharose 66-75 amino acid starvation-responsive transcription factor GCN4 Saccharomyces cerevisiae S288C 44-48 2598093-1 1989 A new platelet aggregating protein (PAP) was purified from the plasma of a patient with acute thrombotic thrombocytopenic purpura (TTP) using A1 (OH)3 adsorption, PEG fractionation, DEAE cellulose chromatography, lentil lectin Sepharose 4B chromatography and gel filtration HPLC. Sepharose 227-236 poly(A) polymerase alpha Homo sapiens 36-39 2550050-5 1989 The fusion protein binds to IgG-Sepharose, and the bound fusion protein undergoes apparent autocleavage in the presence of 4-aminophenylmercuric acetate with elution of active pump-1 species of Mr 21,000 and 19,000. Sepharose 32-41 matrix metallopeptidase 7 Homo sapiens 176-182 2571074-5 1989 Heparin-releasable LPL could be resolved into two fractions by chromatography on con A-Sepharose; this pattern of elution was not affected by the prior treatment of cardiac myocytes with taxol. Sepharose 87-96 lipoprotein lipase Homo sapiens 19-22 2524526-2 1989 Sepharose conjugated anti-CD3, monocytes, and PMA each could induce the p55 component of the IL-2R as well as responsiveness to exogenous IL-2. Sepharose 0-9 interleukin 2 receptor subunit alpha Homo sapiens 72-75 2524526-2 1989 Sepharose conjugated anti-CD3, monocytes, and PMA each could induce the p55 component of the IL-2R as well as responsiveness to exogenous IL-2. Sepharose 0-9 interleukin 2 receptor subunit alpha Homo sapiens 93-98 2524526-2 1989 Sepharose conjugated anti-CD3, monocytes, and PMA each could induce the p55 component of the IL-2R as well as responsiveness to exogenous IL-2. Sepharose 0-9 interleukin 2 Homo sapiens 93-97 2666306-1 1989 The CDw44 glycoprotein was purified from 2.3 x 10(11) CD3+ CD4+ CD8- T-chronic lymphocytic leukaemia (CLL) cells using F10-44-2 monoclonal antibody affinity chromatography, DEAE-Sepharose anion-exchange chromatography, passage down carboxymethyl (CM)-Sepharose cation-exchange columns, wheat germ lectin affinity chromatography and gel-permeation chromatography. Sepharose 178-187 CD44 molecule (Indian blood group) Homo sapiens 4-9 2543451-4 1989 Protein phosphatase 1 bound to phosphorylated inhibitor-1 covalently coupled to Sepharose or Affi-Gel beads but did not bind to immobilized preparations of dephosphorylated inhibitor-1 or bovine serum albumin. Sepharose 80-89 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 46-57 2543451-5 1989 Phosphorylated inhibitor-1 coupled to Sepharose or Affi-Gel beads retained its ability to inhibit protein phosphatase 1, although the apparent IC50 was decreased about 500-fold. Sepharose 38-47 protein phosphatase 1 regulatory inhibitor subunit 1A Homo sapiens 15-26 2721453-6 1989 Analysis of [3H]mannose-labeled oligosaccharides on Concanavalin-A-agarose showed 85% binding for those from TSH alpha, 70% for free alpha, and 50% for those from TSH beta. Sepharose 67-74 glycoprotein hormones, alpha subunit Mus musculus 109-118 2591923-1 1989 Apo E was purified from the very low density lipoprotein (VLDL) of normal and hypertriglyceridemic subjects by Sephadex G-75, heparin-Sepharose 4B affinity chromatography, and preparative SDS polyacrylamide gel electrophoresis. Sepharose 134-143 apolipoprotein E Capra hircus 0-5 2666306-1 1989 The CDw44 glycoprotein was purified from 2.3 x 10(11) CD3+ CD4+ CD8- T-chronic lymphocytic leukaemia (CLL) cells using F10-44-2 monoclonal antibody affinity chromatography, DEAE-Sepharose anion-exchange chromatography, passage down carboxymethyl (CM)-Sepharose cation-exchange columns, wheat germ lectin affinity chromatography and gel-permeation chromatography. Sepharose 251-260 CD44 molecule (Indian blood group) Homo sapiens 4-9 2659722-1 1989 A chemotactic protein for polymorphonuclear leukocytes (lung carcinoma-derived chemotaxin [LUCT]) was purified from culture fluid of the human lung giant cell carcinoma LU65C cells to electrophoretically homogeneous form through five sequential purification steps: DEAE-Sepharose, CM-Sepharose, HPLC on carboxyl-methylated-polyvinylalcohol resin, hydrophobic, and reversed-phase. Sepharose 270-279 C-X-C motif chemokine ligand 8 Homo sapiens 56-89 2723649-1 1989 The kinetics of neural cell adhesion molecule (NCAM) binding to heparin were studied in a heparin-Sepharose-based solid-phase binding assay. Sepharose 98-107 neural cell adhesion molecule 1 Rattus norvegicus 16-45 2723649-1 1989 The kinetics of neural cell adhesion molecule (NCAM) binding to heparin were studied in a heparin-Sepharose-based solid-phase binding assay. Sepharose 98-107 neural cell adhesion molecule 1 Rattus norvegicus 47-51 2768222-1 1989 Human microsomal dipeptidase (MDP, formerly referred to as dehydropeptidase-I or renal dipeptidase) [EC 3.4.13.11] was solubilized from the membrane fraction of kidney by treatment with octyl-beta-D-glucoside and purified by a procedure including ion exchange chromatography and affinity chromatography on cilastatin-immobilized Sepharose. Sepharose 329-338 dipeptidase 1 Homo sapiens 6-28 2768222-1 1989 Human microsomal dipeptidase (MDP, formerly referred to as dehydropeptidase-I or renal dipeptidase) [EC 3.4.13.11] was solubilized from the membrane fraction of kidney by treatment with octyl-beta-D-glucoside and purified by a procedure including ion exchange chromatography and affinity chromatography on cilastatin-immobilized Sepharose. Sepharose 329-338 dipeptidase 1 Homo sapiens 30-33 2768222-1 1989 Human microsomal dipeptidase (MDP, formerly referred to as dehydropeptidase-I or renal dipeptidase) [EC 3.4.13.11] was solubilized from the membrane fraction of kidney by treatment with octyl-beta-D-glucoside and purified by a procedure including ion exchange chromatography and affinity chromatography on cilastatin-immobilized Sepharose. Sepharose 329-338 dipeptidase 1 Homo sapiens 59-77 2768222-1 1989 Human microsomal dipeptidase (MDP, formerly referred to as dehydropeptidase-I or renal dipeptidase) [EC 3.4.13.11] was solubilized from the membrane fraction of kidney by treatment with octyl-beta-D-glucoside and purified by a procedure including ion exchange chromatography and affinity chromatography on cilastatin-immobilized Sepharose. Sepharose 329-338 dipeptidase 1 Homo sapiens 81-98 2768222-5 1989 Human MDP was found to bind to Con A-Sepharose and the activity was eluted with methyl-alpha-D-mannopyranoside, suggesting that human MDP is a glycoprotein. Sepharose 37-46 dipeptidase 1 Homo sapiens 6-9 2768222-5 1989 Human MDP was found to bind to Con A-Sepharose and the activity was eluted with methyl-alpha-D-mannopyranoside, suggesting that human MDP is a glycoprotein. Sepharose 37-46 dipeptidase 1 Homo sapiens 134-137 2543820-1 1989 An affinity procedure with purified, biotinylated human transferrin and streptavidin-agarose was used to identify the transferrin-binding proteins in strains of Haemophilus influenzae. Sepharose 85-92 transferrin Homo sapiens 118-129 2659722-1 1989 A chemotactic protein for polymorphonuclear leukocytes (lung carcinoma-derived chemotaxin [LUCT]) was purified from culture fluid of the human lung giant cell carcinoma LU65C cells to electrophoretically homogeneous form through five sequential purification steps: DEAE-Sepharose, CM-Sepharose, HPLC on carboxyl-methylated-polyvinylalcohol resin, hydrophobic, and reversed-phase. Sepharose 270-279 C-X-C motif chemokine ligand 8 Homo sapiens 91-95 2673929-5 1989 The G6PD activities from normal E. coli and from pAC1-transformed cells comigrated with human G6PD when subjected to electrophoresis on agarose gels. Sepharose 136-143 glucose-6-phosphate dehydrogenase Homo sapiens 4-8 2470735-4 1989 The resulting product, called anhydrourokinase, was found to reversibly bind the PAI-1 when immobilized on cyanogen bromide-activated Sepharose 4B beads. Sepharose 134-146 serpin family E member 1 Homo sapiens 81-86 2673929-5 1989 The G6PD activities from normal E. coli and from pAC1-transformed cells comigrated with human G6PD when subjected to electrophoresis on agarose gels. Sepharose 136-143 dual specificity phosphatase 2 Homo sapiens 49-53 2673929-5 1989 The G6PD activities from normal E. coli and from pAC1-transformed cells comigrated with human G6PD when subjected to electrophoresis on agarose gels. Sepharose 136-143 glucose-6-phosphate dehydrogenase Homo sapiens 94-98 2542279-1 1989 Extracellular myeloperoxidase of human myeloid leukemia HL-60 cells was purified to homogeneity from its culture supernatant by ammonium sulfate fractionation, CM-Sepharose column chromatography, and monoclonal antibody-Sepharose affinity column chromatography. Sepharose 163-172 myeloperoxidase Homo sapiens 14-29 2470735-14 1989 It is concluded that fully active PAI-1 in association with vitronectin can be isolated by anhydrourokinase-Sepharose 4B chromatography and that vitronectin is a binding protein for PAI-1 which activates and stabilizes PAI-1. Sepharose 108-117 serpin family E member 1 Homo sapiens 34-39 2470735-14 1989 It is concluded that fully active PAI-1 in association with vitronectin can be isolated by anhydrourokinase-Sepharose 4B chromatography and that vitronectin is a binding protein for PAI-1 which activates and stabilizes PAI-1. Sepharose 108-117 vitronectin Homo sapiens 60-71 2540190-6 1989 Direct evidence that ACP was a subunit of the acyltransferase/synthetase was obtained by the adsorption of both catalytic activities to an ACP-Sepharose affinity column and by the binding of [3H]ACP to the purified enzyme preparation. Sepharose 143-152 CPAT1 Homo sapiens 21-24 2723440-0 1989 Purification of uteroglobin using monospecific antibodies coupled to divinylsulphone-activated agarose. Sepharose 95-102 uteroglobin Oryctolagus cuniculus 16-27 2540172-1 1989 The chicken liver cation-independent mannose 6-phosphate receptor has been purified to apparent homogeneity by affinity chromatography on pentamannose phosphate-Sepharose and tested for its ability to bind iodinated human IGF-I, human IGF-II, and chicken IGF-II. Sepharose 161-170 insulin like growth factor 2 receptor Gallus gallus 18-65 2540190-6 1989 Direct evidence that ACP was a subunit of the acyltransferase/synthetase was obtained by the adsorption of both catalytic activities to an ACP-Sepharose affinity column and by the binding of [3H]ACP to the purified enzyme preparation. Sepharose 143-152 CPAT1 Homo sapiens 139-142 2540190-6 1989 Direct evidence that ACP was a subunit of the acyltransferase/synthetase was obtained by the adsorption of both catalytic activities to an ACP-Sepharose affinity column and by the binding of [3H]ACP to the purified enzyme preparation. Sepharose 143-152 CPAT1 Homo sapiens 139-142 2525480-2 1989 Subcellular fractions were prepared on linear sucrose gradients and rhodopsin was extracted with detergent and purified by chromatography on ConA-Sepharose. Sepharose 146-155 rhodopsin Rattus norvegicus 68-77 2720970-6 1989 When reacted against rabbit anti-sheep myoglobin, two isoforms also appeared as two nonidentical precipitin lines on agarose gel. Sepharose 117-124 myoglobin Ovis aries 39-48 2525480-3 1989 A fraction enriched in rough endoplasmic reticulum (RER) and substantially free of rod outer segments (ROS) was found to contain a light-sensitive protein exhibiting the properties of rhodopsin on ConA-Sepharose or Agarose chromatography and on SDS-polyacrylamide gel electrophoresis, as well as immunologically. Sepharose 202-211 rhodopsin Rattus norvegicus 184-193 2719674-5 1989 The use of a Con A Sepharose affinity column followed by preparative gel electrophoresis resulted in a greater than 250,000 fold purification of osteogenin. Sepharose 19-28 bone morphogenetic protein 3 Bos taurus 145-155 2715292-4 1989 DEAE-trisacryl, chromatofocusing, and anti-SHBG-immunoglobulin Sepharose chromatography resulted in coelution of the androgen- and estrogen-binding activities. Sepharose 63-72 sex hormone binding globulin Homo sapiens 43-47 28305783-7 1989 In non-conditioned medium on agarose, E14 cells formed embryoid bodies which when allowed to reattach differentiated into a wide variety of tissues. Sepharose 29-36 skull morphology 21 Mus musculus 38-41 2566503-1 1989 Partial purification of the dopamine D-2 receptor from bovine striatum, solubilized in the presence of 1% digitonin, was obtained by chromatography on wheat germ lectin agarose. Sepharose 169-176 dopamine receptor D2 Bos taurus 28-49 2496614-7 1989 Prior to treatment, there was a uniform reduction of all the multimers of plasma vWF in sodium dodecyl sulfate agarose gel electrophoresis. Sepharose 111-118 von Willebrand factor Homo sapiens 81-84 2541738-0 1989 Free gp70 from FeLV: enrichment from cell culture fluid by ferric oxide-agarose chromatography. Sepharose 72-79 embigin Homo sapiens 5-9 2541738-1 1989 A new chromatographic material based on beads of macroporous crosslinked agarose containing ferric oxide particles was used for enrichment of gp70--the envelope glycoprotein of feline leukemia virus (FeLV). Sepharose 73-80 embigin Homo sapiens 142-146 2758071-5 1989 Using affinity chromatography on hemoglobin-Sepharose 4B, cathepsin D was purified 300--320-fold. Sepharose 44-53 cathepsin D Rattus norvegicus 58-69 2540128-1 1989 IgM molecules were purified by the use of anti-IgM antibody-coupled Sepharose from the culture supernatant of an Epstein-Barr-virus-transformed lymphoblastoid cell line, MP1, that secretes alloantibodies possessing HLA-DQw2 specificity as defined by the cytotoxicity assay. Sepharose 68-77 late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 Homo sapiens 170-173 2926137-5 1989 Eosinophils released eosinophil-derived neurotoxin when incubated with Sepharose beads coupled to Ig of the IgG or IgA isotypes, as well as IgA-Fc fragments. Sepharose 71-80 ribonuclease A family member 2 Homo sapiens 21-50 2631863-3 1989 Measurement of precursor-protein association by the extent of affinity chromatographic retardation on agarose-immobilized BNPII verified that the semisynthetic precursor with native AVP sequence has an enhanced self-association propensity similar to that predicted for native precursor. Sepharose 102-109 arginine vasopressin Bos taurus 182-185 2713419-4 1989 Further separation of the receptor-transferrin complex from the free transferrin is achieved by gel chromatography on a AcA34-Sepharose 6B separation system. Sepharose 126-135 transferrin Rattus norvegicus 35-46 2517308-1 1989 The major beta(1-3)N-acetylglucosaminyltransferase [beta(1-3)GlcNAc-transferase] activity in human serum was isolated by DEAE- and CM-Sepharose column chromatography. Sepharose 134-143 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 10-18 2517308-1 1989 The major beta(1-3)N-acetylglucosaminyltransferase [beta(1-3)GlcNAc-transferase] activity in human serum was isolated by DEAE- and CM-Sepharose column chromatography. Sepharose 134-143 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 52-60 2649888-3 1989 Through the use of affinity chromatography involving GCN4- or Pol II-Sepharose columns, we show that GCN4 interacts specifically with Pol II in vitro. Sepharose 69-78 amino acid starvation-responsive transcription factor GCN4 Saccharomyces cerevisiae S288C 101-105 2649888-4 1989 Purified Pol II is retained on the GCN4-Sepharose column under conditions in which the vast majority of proteins flow through. Sepharose 40-49 amino acid starvation-responsive transcription factor GCN4 Saccharomyces cerevisiae S288C 35-39 2649888-6 1989 Conversely, GCN4 protein, synthesized in vitro or in Escherichia coli, specifically binds to the Pol II-Sepharose column under equivalent conditions. Sepharose 104-113 amino acid starvation-responsive transcription factor GCN4 Saccharomyces cerevisiae S288C 12-16 2930569-8 1989 The decreased DNA polymerase activity following incubation with phosphatase beads correlated with the binding of the DNA polymerase polypeptides, p185 and p68, to the agarose beads and this binding could not be reversed by either 150 mM potassium chloride or sodium sulfate. Sepharose 167-174 DNA polymerase delta 3, accessory subunit Bos taurus 155-158 2493582-5 1989 Here we report the first isolation of dystrophin from digitonin-solubilized skeletal muscle membranes using wheat germ agglutinin (WGA)-Sepharose. Sepharose 136-145 dystrophin Homo sapiens 38-48 2493582-6 1989 We find that dystrophin is not a glycoprotein but binds to WGA-Sepharose because of its tight association with a WGA-binding glycoprotein. Sepharose 63-72 dystrophin Homo sapiens 13-23 2538159-1 1989 It was revealed that thymidylate kinase was purified together with cytosolic thymidine kinase from human term placenta by p-aminophenyl thymidine-3"-phosphate-CH-Sepharose affinity column chromatography, which has been commonly used for purification of thymidine kinase. Sepharose 162-171 deoxythymidylate kinase Homo sapiens 21-39 2466553-4 1989 CAR-3 antigen was purified by a three-step procedure, consisting of perchloric acid extraction, molecular sieving on Sepharose CL-4B, and affinity chromatography on AR-3 antibodies coupled to Sepharose 4B. Sepharose 192-204 carbonic anhydrase 3 Homo sapiens 0-5 2716051-3 1989 Site-specific antibodies purified by S1-Sepharose readily bound to myosin. Sepharose 40-49 myosin heavy chain 14 Homo sapiens 67-73 2648396-4 1989 In Rat-1a cells the expression of human c-jun mRNA was associated with the ability to clone in soft agarose and form tumors in nude mice. Sepharose 100-107 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 40-45 2648396-6 1989 However, cotransfection of the c-jun constructs with an activated human c-Ha-ras gene led to foci of transformed cells which gave rise to immortalized cell lines that cloned in soft agarose and formed tumors in nude mice. Sepharose 182-189 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 31-36 2538330-11 1989 The glycoprotein nature of the VIP-binding sites of IGR39 cells has been investigated by affinity chromatography on wheat-germ-agglutinin-Sepharose. Sepharose 138-147 vasoactive intestinal peptide Homo sapiens 31-34 2538455-3 1989 We first isolated high affinity 125I-beta-galactosidase by affinity chromatography on an IGF-II/Man-6-P receptor-Sepharose column. Sepharose 113-122 galactosidase beta 1 Homo sapiens 37-55 2538242-5 1989 Clam cdc2 and the associated cyclins bind to p13suc1-Sepharose. Sepharose 53-62 cyclin dependent kinase 1 Homo sapiens 5-9 2502319-5 1989 T-PA was bound to both tauBSA and putBSA, which were immobilized on agarose beads, with KD values of 163 and 138 nM, respectively. Sepharose 68-75 plasminogen activator, tissue type Homo sapiens 0-4 2522013-6 1989 Limited elastase proteolysis of plasminogen and plasmin resulted in the generation of two distinct thrombospondin binding domains, one of which was retained on lysine-agarose. Sepharose 167-174 plasminogen Homo sapiens 32-39 2465100-2 1989 After addition of a low concentration of thrombin to normal plasma, a progressive and significant increase in crosslinked fibrin polymers was found by sodium dodecyl sulfate agarose gel electrophoresis, reaching 27% of total fibrinogen and fibrin before gel formation. Sepharose 174-181 coagulation factor II, thrombin Homo sapiens 41-49 2540912-10 1989 The soluble ANP-Rs from VSMC and olfactory were chromatographically indistinguishable on phenyl-, DEAE-, and wheat germ agglutinin-agarose columns. Sepharose 131-138 natriuretic peptide A Rattus norvegicus 12-15 2543489-6 1989 The transferrin- and lactoferrin-binding proteins were identified after affinity isolation using biotinylated human transferrin or lactoferrin and streptavidin-agarose. Sepharose 160-167 transferrin Homo sapiens 4-15 2546687-1 1989 Bovine lung thrombomodulin was partially purified, and immobilized on agarose gel (Sepharose 4B). Sepharose 70-77 thrombomodulin Bos taurus 12-26 2546687-1 1989 Bovine lung thrombomodulin was partially purified, and immobilized on agarose gel (Sepharose 4B). Sepharose 83-95 thrombomodulin Bos taurus 12-26 2783905-5 1989 Heparin-Sepharose fractionation of cell extracts revealed that bone cells contained a basic FGF (bFGF)-like molecule, that displayed high affinity for heparin. Sepharose 8-17 fibroblast growth factor 2 Bos taurus 86-95 2783905-5 1989 Heparin-Sepharose fractionation of cell extracts revealed that bone cells contained a basic FGF (bFGF)-like molecule, that displayed high affinity for heparin. Sepharose 8-17 fibroblast growth factor 2 Bos taurus 97-101 2523465-11 1989 Following analysis of alpha-L-fucosidase, the agarose gel can be chemically developed to reveal the PGM1 subtyping pattern. Sepharose 46-53 phosphoglucomutase 1 Homo sapiens 100-104 2924890-4 1989 TNF-BP was purified 1,000,000-fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF-Sepharose and reverse phase chromatography. Sepharose 170-179 tumor necrosis factor Homo sapiens 0-3 2521859-7 1989 Both forms co-eluted with the sole lipoprotein lipase activity peak from heparin-Sepharose; this indicates that both were dimeric. Sepharose 81-90 lipoprotein lipase Cavia porcellus 35-53 2732214-1 1989 Extracellular phospholipase A2 was purified about 1.7 X 10(5) fold to near homogeneity from human synovial fluid of rheumatoid arthritis by sequential use of column chromatographies on heparin-Sepharose, butyl-Toyopearl, and reversed-phase HPLC. Sepharose 193-202 phospholipase A2 group IB Rattus norvegicus 14-30 2537357-7 1989 mAb 3C7.14 coupled to Sepharose could efficiently immunoaffinity purify TGF-beta 2 from a complex mixture of proteins. Sepharose 22-31 transforming growth factor beta 2 Homo sapiens 72-82 2493788-2 1989 Separation of active from inactive inhibitor on anhydrochymotrypsin-sepharose yields a form which has a second order association rate with neutrophil elastase which is approximately one half that for the native protein. Sepharose 68-77 elastase, neutrophil expressed Homo sapiens 139-158 2704375-2 1989 IgA1 and all galactose containing fragments bound to jacalin-Sepharose, including Fab fragments containing only the galNac linked to serine-224 and Fc fragments containing four gal-galNac sequences. Sepharose 61-70 immunoglobulin heavy constant alpha 1 Homo sapiens 0-4 2922394-5 1989 The phosphorylated receptor, like the native receptor, bound to wheat germ lectin and hCG-Sepharose and migrated as a single band of Mr 90,000 (testis) and Mr 85,000 (ovary) on NaDodSO4/PAGE. Sepharose 90-99 chorionic gonadotropin subunit beta 5 Homo sapiens 86-89 2735254-1 1989 Lactate dehydrogenase-C4 (LDH-C4) from human seminal plasma was purified by 40-60% (NH4)2SO4 precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Sepharose 116-125 lactate dehydrogenase C Homo sapiens 0-24 2735254-1 1989 Lactate dehydrogenase-C4 (LDH-C4) from human seminal plasma was purified by 40-60% (NH4)2SO4 precipitation, Oxamate-Sepharose 4B affinity column chromatography and AMP affinity column chromatography. Sepharose 116-125 lactate dehydrogenase C Homo sapiens 26-32 2464594-5 1989 Size fractionation of the mRNA by agarose gel electrophoresis under non-denaturing conditions resulted in the identification of a 1.5-2.0-kilobase fraction capable of inducing type I 5"-deiodinase activity. Sepharose 34-41 deiodinase, iodothyronine type 1 L homeolog Xenopus laevis 176-196 2713997-6 1989 Likewise, the isoelectrofocussing of purified non-reduced patient"s fibrinogen in agarose gel showed an abnormal distribution in its focussed bands, especially in a group which focussed in a pI-interval between 5.20-5.85. Sepharose 82-89 fibrinogen beta chain Homo sapiens 68-78 2749590-4 1989 Analysis by crossed-immunoelectrophoresis (CIE) in the presence of heparin and affinity chromatography on heparin-Sepharose revealed that the propositus" AT III did not bind to heparin. Sepharose 114-123 serpin family C member 1 Homo sapiens 154-160 2523733-1 1989 Quantitative agarose gel assays reveal that the recA protein promoted renaturation of complementary DNA strands (phi X DNA) proceeds in two stages. Sepharose 13-20 RAD51 recombinase Homo sapiens 48-52 2492518-3 1989 It coprecipitates with C2 on polyethylene glycol fractionation and specifically binds, like C2, to Sepharose-bound iC4/C4b. Sepharose 99-108 complement C4B (Chido blood group) Homo sapiens 119-122 2662807-2 1989 Substrates immobilized by adsorption onto nitrocellulose membranes or by incorporation into agarose gel slabs are suitable for fluorescence zymography after electrophoretic separation of catalytically active proteases, including cathepsin D. Sepharose 92-99 cathepsin D Homo sapiens 229-240 2536691-4 1989 Moreover, fractionation of extracts of mouse 3T3 fibroblasts over an IgE-Sepharose affinity column showed that CBP35 bound to IgE; this binding was reversed by addition of lactose. Sepharose 73-82 lectin, galactose binding, soluble 3 Mus musculus 111-116 2536691-5 1989 Conversely, fractionation of extracts of rat basophilic leukemia cells over an affinity column of Sepharose derivatized with N-(epsilon-amino-caproyl)-D-galactosamine showed that epsilon BP was a galactose-binding protein. Sepharose 98-107 galectin 3 Rattus norvegicus 179-189 2536691-6 1989 Furthermore, epsilon BP bound to IgE-Sepharose could be eluted by lactose. Sepharose 37-46 galectin 3 Rattus norvegicus 13-23 2914904-2 1989 A membrane-bound neutral carboxypeptidase B-like enzyme was solubilized from human placental microvilli with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) and purified to homogeneity by ion-exchange chromatography and affinity chromatography on arginine-Sepharose. Sepharose 275-284 carboxypeptidase B1 Homo sapiens 25-43 2495826-3 1989 Photoaffinity labeling of LDL with inner ring-labeled [125I]T4, followed by SDS-PAGE or agarose-SDS-PAGE of the labeled products, revealed that apoB-100 and its proteolytic cleavage products, apoB-74 and apoB-26, bound [125I]T4. Sepharose 88-95 apolipoprotein B Homo sapiens 144-152 2463100-4 1989 Anti-CD19 McAb inhibited B-cell proliferation induced by anti-IgM coupled to Sepharose beads. Sepharose 77-86 CD19 molecule Homo sapiens 5-9 2463875-5 1989 A Concanavalin A (Con A) sepharose affinity chromatogram was performed to detect the component of AFP in this case. Sepharose 25-34 alpha fetoprotein Homo sapiens 98-101 2703017-11 1989 Evidence that H1-2D4, a mAb directed against the antigen receptor on HPB-ALL cells, and E-PHA interact with a common molecule includes: (a) immunoprecipitation of TcR with H1-2D4 from the glycopeptide fraction specifically eluted from insolubilized lectin with N-acetylgalactosamine; and (b) adsorption of TcR from a solubilized H1-2D4 immunoprecipitate by E-PHA-agarose. Sepharose 363-370 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 163-166 2541934-8 1989 A polypeptide reacting with hemopexin-Sepharose was estimated to have a molecular weight of 80,000, as determined by polyacrylamide gel electrophoresis, in the presence of sodium dodecylsulfate. Sepharose 38-47 hemopexin Homo sapiens 28-37 2914370-7 1989 Electrophoresis of the selectively amplified-restricted apolipoprotein-CIII DNA on agarose gel provides a rapid diagnostic test that allows identification of the subjects with this allele in less than a day. Sepharose 83-90 apolipoprotein C3 Homo sapiens 56-75 2912683-1 1989 The activated glucocorticoid-receptor complexes (GRC) from rat liver bind tightly to histone (from calf thymus)-agarose and cannot be eluted with 3 M KCl or 50% ethylene glycol, but can be eluted with 20 mM pyridoxal 5"-phosphate (PALP). Sepharose 112-119 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 14-37 2912694-4 1989 Higher salt concentrations were required to disrupt the binding of ASTP to histone-agarose than to disrupt its binding to the anion exchanger diethylaminoethyl-cellulose (half-maximal concentration for inhibition: 200 mM vs. 60 mM KCl). Sepharose 83-90 glycerol kinase Rattus norvegicus 67-71 2912694-5 1989 ATP decreased the binding of ASTP to histone-agarose in a dose-dependent fashion, but ADP and AMP had no appreciable effect on the binding. Sepharose 45-52 glycerol kinase Rattus norvegicus 29-33 2536739-2 1989 In the present studies the serum receptor was affinity labeled with 125I-IGF-II after its adsorption onto pentamannosyl 6-phosphate-Sepharose, demonstrating that it can also bind both ligands simultaneously. Sepharose 132-141 insulin like growth factor 2 Homo sapiens 73-79 2647500-2 1989 The purification of the human proteinase was achieved by solubilization of the enzyme from membranes with 2% sodium deoxycholate followed by two consecutive passages through an Agarose A-1.5 m column. Sepharose 177-184 endogenous retrovirus group K member 25 Homo sapiens 30-40 2912694-6 1989 Pyridoxal 5"-phosphate (10 mM) inhibited the binding of ASTP to histone-agarose, whereas 10 mM pyridoxal, pyridoxamine 5"-phosphate, pyridoxamine, and pyridoxine were not inhibitory. Sepharose 72-79 glycerol kinase Rattus norvegicus 56-60 2536357-6 1989 This result is supported by affinity chromatography with CPE immobilized on Sepharose 4B, which showed the specific interaction of similar size proteins with CPE. Sepharose 76-85 carboxypeptidase E Chlorocebus sabaeus 57-60 2912694-8 1989 In contrast, ASTP pretreated in the same way could bind to histone-agarose. Sepharose 67-74 glycerol kinase Rattus norvegicus 13-17 2536357-6 1989 This result is supported by affinity chromatography with CPE immobilized on Sepharose 4B, which showed the specific interaction of similar size proteins with CPE. Sepharose 76-85 carboxypeptidase E Chlorocebus sabaeus 158-161 2521492-7 1989 CALLA antigen purified from the NALM-6 leukemic cell line by affinity to 44C10-IgG Sepharose retained a peptidase activity that was completely blocked by thiorphan and phosphoramidon. Sepharose 83-92 membrane metalloendopeptidase Homo sapiens 0-5 2465355-3 1989 RBP was purified from rat serum, loaded with [3H]retinol, and the [3H]retinol-RBP complex purified by affinity chromatography on human transthyretin-Sepharose. Sepharose 149-158 retinol binding protein 4 Homo sapiens 0-3 2918042-7 1989 Antibodies against aFGF immobilized on protein A-Sepharose were able to specifically and completely remove mitogenic activity from solutions containing aFGF but had no effect on removal of mitogenic activity from control solutions containing bFGF or EGF. Sepharose 49-58 fibroblast growth factor 1 Bos taurus 19-23 2918042-7 1989 Antibodies against aFGF immobilized on protein A-Sepharose were able to specifically and completely remove mitogenic activity from solutions containing aFGF but had no effect on removal of mitogenic activity from control solutions containing bFGF or EGF. Sepharose 49-58 fibroblast growth factor 1 Bos taurus 152-156 2465355-3 1989 RBP was purified from rat serum, loaded with [3H]retinol, and the [3H]retinol-RBP complex purified by affinity chromatography on human transthyretin-Sepharose. Sepharose 149-158 retinol binding protein 4 Homo sapiens 78-81 2915979-2 1989 The factor, provisionally termed keratinocyte growth factor (KGF) because of its predominant activity on this cell type, was purified to homogeneity by a combination of ultrafiltration, heparin-Sepharose affinity chromatography, and hydrophobic chromatography on a C4 reversed-phase HPLC column. Sepharose 194-203 fibroblast growth factor 7 Homo sapiens 33-59 2926296-4 1989 An important finding was the presence of glycosylated rPRL and rGH: indeed Concanavalin A-Sepharose 4B affinity chromatography indicated that 26,000 rPRL and 24,000 rGH display a very strong affinity for lectin. Sepharose 90-99 prolactin Rattus norvegicus 149-153 2915979-2 1989 The factor, provisionally termed keratinocyte growth factor (KGF) because of its predominant activity on this cell type, was purified to homogeneity by a combination of ultrafiltration, heparin-Sepharose affinity chromatography, and hydrophobic chromatography on a C4 reversed-phase HPLC column. Sepharose 194-203 fibroblast growth factor 7 Homo sapiens 61-64 2713351-4 1989 Application of linear gradients to GBF-loaded gelatin-agarose columns resulted in peak elution of the fragment at pH 5.2 or 10.2, at 0.4 M dimethylformamide, 0.9 M GdmCl, or 2.0 M urea, conditions far short of those required to induce structural changes detectable by fluorescence or circular dichroism. Sepharose 54-61 Kruppel like factor 6 Homo sapiens 35-38 2660367-1 1989 Uracil-DNA glycosylase (UDG) purified by various procedures from the human placenta was used to obtain immune antisera with specific antibodies, the antibodies being affinity-purified on UDG-sepharose. Sepharose 191-200 uracil DNA glycosylase Homo sapiens 0-22 2660367-1 1989 Uracil-DNA glycosylase (UDG) purified by various procedures from the human placenta was used to obtain immune antisera with specific antibodies, the antibodies being affinity-purified on UDG-sepharose. Sepharose 191-200 uracil DNA glycosylase Homo sapiens 24-27 2486008-1 1989 Cathepsin H was isolated from human placenta by autolysis, acetone fractionation, and chromatography on DEAE-cellulose, Sephadex G-75, hydroxyapatite and concanavalin A-Sepharose. Sepharose 169-178 cathepsin H Homo sapiens 0-11 2492196-1 1989 Two non-amino acid components as well as the glutathione constituents in labile associations with transthyretin (TTR) have been detected by preparative polyacrylamide gel electrophoresis from preparations isolated by affinity chromatography on Sepharose-bound retinol-binding protein (RBP). Sepharose 244-253 transthyretin Homo sapiens 98-111 2492196-1 1989 Two non-amino acid components as well as the glutathione constituents in labile associations with transthyretin (TTR) have been detected by preparative polyacrylamide gel electrophoresis from preparations isolated by affinity chromatography on Sepharose-bound retinol-binding protein (RBP). Sepharose 244-253 transthyretin Homo sapiens 113-116 2912448-1 1989 The clathrin binding domain of the assembly protein AP-2 has been identified by proteolytically cleaving AP-2 into 2 discrete moieties, termed light and heavy mero-AP (LM-AP and HM-AP), and testing their ability to bind to clathrin assembled into cage structures or to clathrin trimers immobilized on Sepharose. Sepharose 301-310 transcription factor AP-2 alpha Homo sapiens 52-56 2909517-8 1989 Cell-free activation of the nonactivated receptor-antibody complexes immobilized on protein A-Sepharose minicolumns allowed the recovery of the Hsp 90 dissociated from the complexes during activation. Sepharose 94-103 heat shock protein, 2 Mus musculus 144-150 2566905-9 1989 Purified liver transglutaminase strongly bound to Tb(III)-Chelating Sepharose beads and binding could not be disrupted by 100 mM CaCl2 solution. Sepharose 68-77 protein-glutamine gamma-glutamyltransferase 2 Cavia porcellus 15-31 2535959-5 1989 Both antibodies also reacted with an integrin-related fibronectin-binding receptor complex purified by ligand affinity chromatography on fibronectin-Sepharose columns. Sepharose 149-158 fibronectin 1 Mus musculus 54-65 2535959-5 1989 Both antibodies also reacted with an integrin-related fibronectin-binding receptor complex purified by ligand affinity chromatography on fibronectin-Sepharose columns. Sepharose 149-158 fibronectin 1 Mus musculus 137-148 2463252-4 1989 One of these proteins was purified approximately 425-fold from ammonium sulfate-fractionated plasma using standard chromatographic procedures together with affinity chromatography on PAI-1-Sepharose. Sepharose 189-198 serpin family E member 1 Bos taurus 183-188 2706244-1 1989 The existence of putative metal binding sites on the estradiol receptor (ER) molecule from calf uterus was evaluated by immobilizing various divalent metals to iminodiacetate-Sepharose. Sepharose 175-184 estrogen receptor 1 Bos taurus 53-71 2706244-1 1989 The existence of putative metal binding sites on the estradiol receptor (ER) molecule from calf uterus was evaluated by immobilizing various divalent metals to iminodiacetate-Sepharose. Sepharose 175-184 estrogen receptor 1 Bos taurus 73-75 2706244-4 1989 Analysis of affinity-labeled ER by [3H]tamoxifen aziridine after elution from a column of Zn(II)-charged iminodiacetate-Sepharose showed that ER fragments obtained by extensive trypsinization were also bound. Sepharose 120-129 estrogen receptor 1 Bos taurus 29-31 2706244-4 1989 Analysis of affinity-labeled ER by [3H]tamoxifen aziridine after elution from a column of Zn(II)-charged iminodiacetate-Sepharose showed that ER fragments obtained by extensive trypsinization were also bound. Sepharose 120-129 estrogen receptor 1 Bos taurus 142-144 2914605-1 1989 A high molecular mass latent form of transforming growth factor type-beta (TGF-beta) was purified to homogeneity from rat platelets by a seven-step procedure involving group-specific affinity chromatographies on Red-Toyopearl and zinc chelating-Sepharose. Sepharose 245-254 transforming growth factor, beta 1 Rattus norvegicus 75-83 2556985-1 1989 Three forms of collagenase inhibitor were isolated; one bound to Con A-Sepharose and the other two did not. Sepharose 71-80 TIMP metallopeptidase inhibitor 2 Bos taurus 15-36 2558505-5 1989 Human kallistatin has been purified from serum, using chromatographic steps including DEAE-Sephadex, hydroxylapatite, Cibacron blue-Sepharose, Sephacryl S200, and preparative polyacrylamide gel electrophoresis. Sepharose 132-141 serpin family A member 4 Homo sapiens 6-17 2558510-5 1989 We purified one ACE from the soluble fraction by lisinopril-linked Sepharose 6B affinity column chromatography and Cellulofine GCL-200 gel filteration. Sepharose 67-76 angiotensin I converting enzyme Homo sapiens 16-19 2783543-7 1989 Both gel filtration and heparin-Sepharose affinity chromatography separate the cytosolic androgen receptor from MEB. Sepharose 32-41 androgen receptor Rattus norvegicus 89-106 2665407-5 1989 Prior to determination of proinsulin-binding IgG, the insulin-binding IgG was removed by means of sepharose-bound insulin according to the method of Heding. Sepharose 98-107 insulin Homo sapiens 54-61 2558531-3 1989 Using 0.5 M NaCl, 10 mM ATP and 5 mM adenosine as eluting agents, it was possible to separate on AMP-sepharose column AMP deaminase "high Km" and "low Km" 5"-nucleotidase and adenosine kinase. Sepharose 101-110 adenosine kinase Homo sapiens 175-191 2521276-6 1989 Crossed immunoelectrophoresis and sodium dodecyl sulfate (SDS)-agarose gel electrophoresis revealed that the large vWf multimers were either absent from or relatively decreased in all patients except one. Sepharose 63-70 von Willebrand factor Homo sapiens 115-118 2917133-4 1989 The antithrombin was isolated by heparin Sepharose affinity chromatography. Sepharose 41-50 serpin family C member 1 Homo sapiens 4-16 2920013-2 1989 By affinity chromatography on a concanavalin A-Sepharose column in well-defined conditions, human serotransferrin isolated from healthy donors was resolved into three carbohydrate molecular variants: Tf-I (less than 1%), Tf-II (17 +/- 2%) and Tf-III (82 +/- 3%) containing two triantennary glycans, one triantennary and one biantennary glycans and two biantennary glycans respectively. Sepharose 47-56 transferrin Homo sapiens 98-113 2917133-7 1989 The antithrombin was further purified by chromatography on thrombin-Sepharose (to remove the normal component), reduced, S-carboxymethylated and fragmented with cyanogen bromide. Sepharose 68-77 serpin family C member 1 Homo sapiens 4-16 2611892-7 1989 Perhaps also of general use is the finding that either myosin-II or alpha-actinin in crude cell extracts can be bound selectively to the poly-L-proline agarose column depending on the ionic conditions used to equilibrate the column. Sepharose 152-159 actinin alpha 1 Homo sapiens 68-81 2642767-8 1989 ADR activity can be removed from active extracts by absorption with aprotinin-conjugated agarose beads, and can be removed from the beads by elution at pH 5.0. Sepharose 89-96 aldo-keto reductase family 1 member B Homo sapiens 0-3 2805645-4 1989 A method utilizing heparin-agarose affinity chromatography was more efficient for purifying lactoferrin than a method including gel filtration, ion exchange chromatography and a second gel filtration. Sepharose 27-34 lactotransferrin Bos taurus 92-103 2783250-3 1989 The IgG fractions from normal rabbit serum and the anti-CSF serum were bound to cyanogen bromide-activated sepharose. Sepharose 107-116 colony stimulating factor 2 Homo sapiens 56-59 2783250-8 1989 In a further purification step, the CSF was retained selectively by concanavalin A sepharose and eluted with alpha-methylglucoside. Sepharose 83-92 colony stimulating factor 2 Homo sapiens 36-39 2511167-6 1989 The inhibitory activity disappeared when the extract was passed through a column with sepharose-coupled anti-PAI-1 IgG, while the run-through from a similar column coupled with preimmune IgG still contained the inhibitor. Sepharose 86-95 serpin family E member 2 Rattus norvegicus 109-114 2535484-0 1989 Structural assessment of the N-linked oligosaccharides of cell-CAM 105 by lectin-agarose affinity chromatography. Sepharose 81-88 CEA cell adhesion molecule 1 Rattus norvegicus 58-70 2535484-1 1989 The N-linked oligosaccharides of cell-CAM 105, a glycoprotein involved in the intercellular adhesion between rat hepatocytes, were studied by sequential lectin-agarose affinity chromatography of desialylated, [14C]-labelled glycopeptides. Sepharose 160-167 CEA cell adhesion molecule 1 Rattus norvegicus 33-45 2484884-4 1989 When bound to Sepharose beads, HIS42 induces T cell proliferation in the presence of interleukin-2. Sepharose 14-23 interleukin 2 Rattus norvegicus 85-98 2691398-1 1989 Monoclonal anti-GnRH antibodies reacting to the heptapeptide 4-10 (H-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-OH) were isolated by affinity chromatography using Sepharose 4B-heptapeptide (4-10) column. Sepharose 148-157 gonadotropin releasing hormone 1 Homo sapiens 16-20 2807579-5 1989 Using BIP 1 coupled to Sepharose 4B as reverse immunosorbent, Bet v I was obtained in a single-step procedure and characterized as single band by SDS-PAGE. Sepharose 23-32 delta/notch-like EGF repeat containing Mus musculus 62-65 2470907-6 1989 Likewise, exogenous IL 1 and anti Leu 4 antibody bound to Sepharose beads independently or cooperatively failed to propagate T cells from low responders. Sepharose 58-67 interleukin 1 alpha Homo sapiens 20-24 2646299-2 1989 The MAF was purified from the cell line culture supernatant by concentration, CM-Sepharose and lentil lectin Sepharose chromatography, AcA 54 gel filtration, Mono Q FPLC and reverse phase HPLC. Sepharose 81-90 avian musculoaponeurotic fibrosarcoma oncogene homolog Mus musculus 4-7 2464688-2 1989 Mouse AFP was isolated from amniotic fluid and purified by polyacrylamide gel electrophoresis followed by Blue Sepharose chromatography. Sepharose 111-120 alpha fetoprotein Mus musculus 6-9 2674322-5 1989 The expression of FSHA and MSHA correlated very well with the direct binding of vibrios to fucose- and mannose-coated agarose beads, respectively. Sepharose 118-125 glycoprotein hormones, alpha polypeptide Homo sapiens 18-22 2918245-5 1989 Agarose gel electrophoresis of type 1-VLDL showed beta mobility, and that of type 2-VLDL showed pre-beta mobility. Sepharose 0-7 CD320 antigen Mus musculus 38-42 2759622-2 1989 alpha 1B phenotyping was done by two-dimensional agarose gel (pH 5.4)-horizontal polyacrylamide gel (pH 9.0) electrophoresis followed by protein staining. Sepharose 49-56 alpha-1-B glycoprotein Homo sapiens 0-8 2461944-1 1988 Antibodies to purified glycerol-3-phosphate dehydrogenase were raised in rabbits and purified from serum by affinity chromatography on enzyme-bound Sepharose columns. Sepharose 148-157 glycerol-3-phosphate dehydrogenase 1 Rattus norvegicus 23-57 2475734-3 1989 Western-blotting of the fractions from the heparin-Sepharose column with a monoclonal antibody prepared against postheparin plasma H-TGL and which binds to an epitope in the carboxyl-terminus of H-TGL gave a single immunoreactive protein band of 65 kDa. Sepharose 51-60 lipase C, hepatic type Homo sapiens 131-136 2475734-3 1989 Western-blotting of the fractions from the heparin-Sepharose column with a monoclonal antibody prepared against postheparin plasma H-TGL and which binds to an epitope in the carboxyl-terminus of H-TGL gave a single immunoreactive protein band of 65 kDa. Sepharose 51-60 lipase C, hepatic type Homo sapiens 195-200 2662173-3 1989 CPB preparations are loaded onto a column of iminodiacetic acid-Sepharose that has been saturated with ZnCl2. Sepharose 64-73 carboxypeptidase B1 Homo sapiens 0-3 2466326-5 1989 Agarose stimulation increased the S-protein level in supernatants from monocyte but not from macrophage cultures. Sepharose 0-7 vitronectin Homo sapiens 34-43 2648135-1 1989 Human monocyte-derived neutrophil chemotactic factor (MDNCF) was purified from culture supernatant of lipopolysaccharide-stimulated human peripheral blood mononuclear leukocytes on a column of Sepharose-bound murine monoclonal anti-MDNCF. Sepharose 193-202 C-X-C motif chemokine ligand 8 Homo sapiens 6-52 2648135-1 1989 Human monocyte-derived neutrophil chemotactic factor (MDNCF) was purified from culture supernatant of lipopolysaccharide-stimulated human peripheral blood mononuclear leukocytes on a column of Sepharose-bound murine monoclonal anti-MDNCF. Sepharose 193-202 C-X-C motif chemokine ligand 8 Homo sapiens 54-59 2561640-1 1989 Membrane receptors for ceruloplasmin were isolated by affinity chromatography using CP bound to CNBr-activated Sepharose 4B. Sepharose 111-120 ceruloplasmin Homo sapiens 23-36 2467400-3 1988 Two molecular forms of plasminogen, Glu- and Lys-plasminogen, induced a dose-dependent reduction of the electrophoretic mobility of HRG, with a half maximal retardation for both plasminogens at 0.50-0.55 microM of added plasminogen to the agarose gel. Sepharose 239-246 histidine rich glycoprotein Homo sapiens 132-135 2848823-3 1988 Sepharose 4B gel filtration chromatography was utilized to demonstrate the formation of specific binding complexes between 125I-VIP and solubilized receptors. Sepharose 0-9 vasoactive intestinal peptide Rattus norvegicus 128-131 3198644-2 1988 We have constructed a series of actins containing mutations at Asp3 and Asp11 and tested these mutant proteins for their ability to bind to DNase I-agarose, polymerize with rabbit skeletal muscle actin, undergo amino-terminal processing, and bind to the myosin-S1 subfragment. Sepharose 148-155 deoxyribonuclease-1 Oryctolagus cuniculus 140-147 2781220-6 1989 All PLAP activity in placental extracts and LAP activity in liver extracts was bound to Con A-Sepharose. Sepharose 94-103 alkaline phosphatase, placental Homo sapiens 4-8 2781220-6 1989 All PLAP activity in placental extracts and LAP activity in liver extracts was bound to Con A-Sepharose. Sepharose 94-103 LAP Homo sapiens 5-8 2781220-7 1989 In the tumor extracts, only 50-70% of the PLAP-like enzyme and 20-50% of the LAP activity from seminomas were bound to Con A-Sepharose. Sepharose 125-134 alkaline phosphatase, placental Homo sapiens 42-46 3266380-2 1988 Plasma vWF was hydrolysed by S aureus V-8 protease (V-8 protease) and the cleaved fragments separated by SDS-agarose gel electrophoresis followed by staining with 125I-labeled polyclonal or monoclonal antibodies against vWF and autoradiography. Sepharose 109-116 von Willebrand factor Homo sapiens 7-10 2973814-4 1988 Following labeling, the homogeneity and integrity of Lp(a) was shown by agarose electrophoresis and immunoblotting. Sepharose 72-79 lipoprotein(a) Homo sapiens 53-58 2848805-2 1988 A human placental soluble "high Km" 5"-nucleotidase has been separated from "low Km" 5"-nucleotidase and nonspecific phosphatase by AMP-Sepharose affinity chromatography. Sepharose 136-145 5'-nucleotidase ecto Homo sapiens 36-51 3143721-1 1988 The D2-dopamine receptor from bovine anterior pituitary has been purified approximately 33,000-fold to apparent homogeneity by sequential use of affinity chromatography on immobilized carboxymethyleneoximinospiperone-Sepharose, Datura stramonium lectin-agarose, and hydroxylapatite chromatography. Sepharose 217-226 dopamine receptor D2 Bos taurus 4-24 3198644-7 1988 However, the amount of in vitro-synthesized actin capable of binding to DNase I-agarose with high affinity or undergoing amino-terminal processing was reduced significantly relative to the wild type actin synthesized in vitro. Sepharose 80-87 deoxyribonuclease-1 Oryctolagus cuniculus 72-79 3198924-4 1988 HSA and BSA did not interfere with the galNac-dependent binding of IgA1, but inhibited the "alternative" binding of IgA1 to jacalin-Sepharose, probably by competition. Sepharose 132-141 immunoglobulin heavy constant alpha 1 Homo sapiens 116-120 3191488-3 1988 While the parental cell line responded to TGF-beta with an inhibition of cellular proliferation in monolayer culture and in soft agarose, an increase in extracellular fibronectin, and a down-regulation of c-myc protooncogene expression, these responses were absent or attenuated in the sublines. Sepharose 129-136 transforming growth factor beta 1 Homo sapiens 42-50 3198924-6 1988 Washing of jacalin-Sepharose columns with excess BSA could disrupt the "alternative" binding and subsequent washing with 0.8 M D-galactose in 0.5 M NaCl/PBS was sufficient to elute all IgA1. Sepharose 19-28 immunoglobulin heavy constant alpha 1 Homo sapiens 185-189 3214328-0 1988 Removal of apolipoprotein B from dog whole blood by ex vivo hemoadsorption on antibody-agarose beads. Sepharose 87-94 apolipoprotein B Canis lupus familiaris 11-27 2973461-10 1988 We have demonstrated by use of calmodulin-Sepharose chromatography that both the calmodulin-dependent and independent Ca2+-ATPase forms bind calmodulin, even though stimulation of activity is seen only with the former one. Sepharose 42-51 calmodulin 1 Homo sapiens 31-41 2973461-10 1988 We have demonstrated by use of calmodulin-Sepharose chromatography that both the calmodulin-dependent and independent Ca2+-ATPase forms bind calmodulin, even though stimulation of activity is seen only with the former one. Sepharose 42-51 calmodulin 1 Homo sapiens 81-91 2973461-10 1988 We have demonstrated by use of calmodulin-Sepharose chromatography that both the calmodulin-dependent and independent Ca2+-ATPase forms bind calmodulin, even though stimulation of activity is seen only with the former one. Sepharose 42-51 calmodulin 1 Homo sapiens 81-91 3242489-6 1988 RTP-1 proteins strongly reacted with anti-MUP-1 A serum on agarose gel electrophoretograms. Sepharose 59-66 receptor (chemosensory) transporter protein 1 Rattus norvegicus 0-5 3242489-6 1988 RTP-1 proteins strongly reacted with anti-MUP-1 A serum on agarose gel electrophoretograms. Sepharose 59-66 major urinary protein 1 Rattus norvegicus 42-47 2977379-2 1988 S-IL-2R protein was obtained from the conditioned medium of L cells transfected with a mutant cDNA clone encoding the extracytoplasmic portion of the IL-2 receptor (IL-2R) and was purified to homogeneity by an IL-2-coupled sepharose column, following by reverse phase chromatography (HPLC). Sepharose 223-232 interleukin 2 receptor subunit beta Homo sapiens 2-7 2977379-2 1988 S-IL-2R protein was obtained from the conditioned medium of L cells transfected with a mutant cDNA clone encoding the extracytoplasmic portion of the IL-2 receptor (IL-2R) and was purified to homogeneity by an IL-2-coupled sepharose column, following by reverse phase chromatography (HPLC). Sepharose 223-232 interleukin 2 Homo sapiens 2-6 3235097-8 1988 Although both antibody affinity matrices exhibited the same antigen binding capacities, the matrix prepared using carbonyldiimidazole-activated agarose bound the MNDA with a high level of specificity while the matrix prepared from CNBr-activated agarose bound numerous other nuclear proteins. Sepharose 144-151 myeloid cell nuclear differentiation antigen Homo sapiens 162-166 3149640-3 1988 The dominant urokinase inhibitor in chondrocyte culture medium was partially purified by concanavalin A-Sepharose affinity chromatography. Sepharose 104-113 serpin family B member 2 Homo sapiens 13-32 3141586-3 1988 The solubilized P400 protein was purified using Sepharose CL-4B and Con A-Sepharose chromatography. Sepharose 48-57 E1A binding protein p400 Mus musculus 16-20 3183386-6 1988 The TKF from a hybridoma was basic protein and had binding capacity to Con A-Sepharose, whereas TNF had an opposite nature. Sepharose 77-86 fibroblast growth factor receptor 4 Homo sapiens 4-7 2851598-1 1988 Epstein-Barr virus (EBV) nuclear antigen type-1 (EBNA-1) was extracted and purified from Raji cells by chromatography on DNA-Sepharose and Blue-dextran Sepharose. Sepharose 125-134 EBNA-1 Human gammaherpesvirus 4 49-55 3058126-7 1988 A synthetic peptide containing residues 243-251 was specifically bound by agarose-insulin beads. Sepharose 74-81 insulin Homo sapiens 82-89 3143413-5 1988 When solubilized rat brain microsomes were applied to a Sepharose 4B column coupled with the rabbit antibody raised against rat liver microsomal NADPH-cytochrome-c reductase, which reacts with aldehyde reductase from rat brain, the eluted fraction ceased to form [14C]hexadecanol but continued to form [14C]hexadecanal from [14C]hexadecanoic acid. Sepharose 56-65 aldo-keto reductase family 1, member B7 Rattus norvegicus 193-211 3196340-6 1988 After coupling to Sepharose the antibody was able to bind to cytochrome P-450(26) and to decrease the 26-hydroxylation of 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol. Sepharose 18-27 cytochrome P-450 Oryctolagus cuniculus 61-77 3191136-1 1988 The single glutathione S-transferase (EC 2.5.1.18) present in rat erythrocytes was purified to apparent homogeneity by affinity chromatography on glutathione-Sepharose and hydroxyapatite chromatography. Sepharose 158-167 hematopoietic prostaglandin D synthase Rattus norvegicus 11-36 3220918-1 1988 Several problems are associated with the biospecific affinity purification of plasma fibronectin on gelatin-Sepharose. Sepharose 108-117 fibronectin 1 Homo sapiens 85-96 2846447-6 1988 By using a one-step immunoaffinity purification method with 115E cross-linked to protein-A-Sepharose, we purified EBNA-2 to homogeneity, i.e., more than 1,200-fold, from Burkitt lymphoma cell extracts. Sepharose 91-100 EBNA-2 Human gammaherpesvirus 4 114-120 2851331-1 1988 A highly efficient new affinity medium for deoxycytidine kinase, deoxycytidine 5"-tetraphosphate-Sepharose (dCp4-Sepharose), has been constructed. Sepharose 97-106 Lcp65Aa Drosophila melanogaster 108-112 2851331-1 1988 A highly efficient new affinity medium for deoxycytidine kinase, deoxycytidine 5"-tetraphosphate-Sepharose (dCp4-Sepharose), has been constructed. Sepharose 113-122 Lcp65Aa Drosophila melanogaster 108-112 2851331-2 1988 A dCp4-Sepharose column effects a one-step, 19,000-fold, purification to homogeneity of dCyd kinase from the ammonium sulfate fraction of Lactobacillus acidophilus R-26 extract, with 60% recovery. Sepharose 7-16 Lcp65Aa Drosophila melanogaster 2-6 3183380-6 1988 SDS-PAGE of C1q-Sepharose eluates also revealed the presence of a 67,000 protein which was accompanied to varying degrees by a 94,000 constituent. Sepharose 16-25 complement C1q A chain Homo sapiens 12-15 3183380-7 1988 Because similar m.w., 125I-labeled proteins were recovered from C1q-Sepharose columns to which lysed, surface-labeled platelets had been applied, both 94,000 and 67,000 components appear to be platelet membrane constituents. Sepharose 68-77 complement C1q A chain Homo sapiens 64-67 2972318-6 1988 The binding of N,N"-dicyclohexylcarbodiimide to the (Ca2+ + Mg2+)-ATPase greatly reduced its ability to bind to a calmodulin-agarose gel. Sepharose 125-132 calmodulin 1 Homo sapiens 114-124 2847658-6 1988 Cathepsin D-digested placental fibronectin applied to a heparin-agarose column and eluted with a NaCl stepwise gradient (0.1, 0.3, 0.5 M) gave two polypeptides (80-100 and 65 kDa) in the 0.3 M NaCl peak. Sepharose 64-71 cathepsin D Homo sapiens 0-11 2847658-6 1988 Cathepsin D-digested placental fibronectin applied to a heparin-agarose column and eluted with a NaCl stepwise gradient (0.1, 0.3, 0.5 M) gave two polypeptides (80-100 and 65 kDa) in the 0.3 M NaCl peak. Sepharose 64-71 fibronectin 1 Homo sapiens 31-42 3190233-1 1988 NAD-dependent succinic semialdehyde dehydrogenase (EC 1.2.1.24) has been purified to homogeneity from human brain via ion-exchange chromatography and affinity chromatography employing Blue Sepharose and 5"-AMP Sepharose. Sepharose 189-198 aldehyde dehydrogenase 5 family member A1 Homo sapiens 0-49 2975169-4 1988 Digestion of the membrane bound D2 dopamine receptors with neuraminidase prior to solubilisation reduced the ability of the receptors to bind to WGA-agarose (50% of applied receptors bound) whereas digestion with N-acetylglucosaminidase did not significantly affect binding to WGA-agarose. Sepharose 149-156 neuraminidase 1 Bos taurus 59-72 2975169-4 1988 Digestion of the membrane bound D2 dopamine receptors with neuraminidase prior to solubilisation reduced the ability of the receptors to bind to WGA-agarose (50% of applied receptors bound) whereas digestion with N-acetylglucosaminidase did not significantly affect binding to WGA-agarose. Sepharose 281-288 neuraminidase 1 Bos taurus 59-72 2975169-7 1988 Neuraminidase treatment reduced the low-affinity population suggesting that the interaction of oligosaccharides bearing sialic acid with WGA-agarose is of lower affinity and that higher-affinity binding is via N-acetylglucosamine. Sepharose 141-148 neuraminidase 1 Bos taurus 0-13 3075905-1 1988 A simple procedure, involving chromatography on concanavalin A-Sepharose and gel filtration, has been developed for the purification of a prothrombin activator from the venom of the Australian brown snake Pseudonaja textilis textilis. Sepharose 63-72 coagulation factor II, thrombin Homo sapiens 138-149 3149503-1 1988 Native one-chain tissue plasminogen activator (t-PA) was rapidly converted to the two-chain form by trypsin-Sepharose cleavage. Sepharose 108-117 plasminogen activator, tissue type Homo sapiens 17-51 3214431-0 1988 Selective elution of rodent glutathione S-transferases and glyoxalase I from the S-hexyglutathione-Sepharose affinity matrix. Sepharose 99-108 glyoxalase 1 Rattus norvegicus 59-71 3214431-5 1988 The enzymes that bind to this matrix can be eluted, according to their subunit composition, in the order Ya-, Yc-, Yf- and Yb-containing GST; glyoxalase I, also retained by S-hexylglutathione-Sepharose, is eluted after the major GST YbYb peak. Sepharose 192-201 glyoxalase 1 Rattus norvegicus 142-154 3142771-2 1988 Two isoforms of methionine adenosyltransferase (S-adenosylmethionine synthetase), A and B, have been partially purified from Sulfolobus solfataricus, a thermophilic archaebacterium optimally growing at 87 degrees C. The chromatographic procedure, involving hydrophobic chromatography on a phenyl-Sepharose column as a major step, results in 330-fold and 150-fold purification of adenosylmethionine synthetase A and B respectively. Sepharose 296-305 phosphoribosylformylglycinamidine synthase Saccharolobus solfataricus 48-79 3192507-1 1988 An endothelial cell glycoprotein that inhibits the initiation of the coagulation process promoted by tissue factor has been isolated by heparin-sepharose, hydroxyapatite and gel filtration chromatography. Sepharose 144-153 coagulation factor III, tissue factor Homo sapiens 101-114 2976757-1 1988 beta-N-Acetylhexosaminidase (hexosaminidase) I, which has an intermediate charge character between those of hexosaminidases A(alpha beta 2) and B[beta beta)2), was purified 1,500-fold from human placenta by procedures including chromatographies on concanavalin A (Con A)-Sepharose and an immunoadsorbent column. Sepharose 271-280 hexosaminidase D Homo sapiens 0-27 2975761-7 1988 In contrast to the above results, 2.4G2 which was capable of extensively cross-linking Fc gamma R II (2.4G2 bound to Sepharose) or of cross-linking Fc gamma R II to surface IgM (2.4G2 hetero-cross-linked with anti-mu) specifically inhibited B lymphocyte responses to anti-mu and lymphokines. Sepharose 117-126 Fc gamma receptor 2B Rattus norvegicus 87-100 2633562-3 1989 Besides, anomalous CP has no oxidase activity of normal CP and differs from it in electrophoretic mobility in agarose and polyacrylamide gels. Sepharose 110-117 ceruloplasmin Homo sapiens 19-21 2844417-7 1988 H1K from starfish binds strongly to p13-Sepharose and the time course of 1-methyladenine-induced H1K activation, whether assayed in crude extract or on p13-Sepharose beads, is identical. Sepharose 40-49 H3 histone pseudogene 6 Homo sapiens 36-39 3217916-5 1988 Degraded fibrinogen molecules whose A alpha chains all terminated before A alpha #540-554 were isolated from purified fibrinogen preparations by immunoaffinity chromatography on F-102 Sepharose. Sepharose 184-193 fibrinogen beta chain Homo sapiens 9-19 2853471-1 1988 The immunoradiometric assay of rat myeloperoxidase (MPO) was developed based on the usage of the CNBr-activated sepharose as a solid phase for antiMPO and of [125I] IgG (the affinity preparation of antiMPO) as a label. Sepharose 112-121 myeloperoxidase Rattus norvegicus 35-50 2853471-1 1988 The immunoradiometric assay of rat myeloperoxidase (MPO) was developed based on the usage of the CNBr-activated sepharose as a solid phase for antiMPO and of [125I] IgG (the affinity preparation of antiMPO) as a label. Sepharose 112-121 myeloperoxidase Rattus norvegicus 52-55 2844815-2 1988 A cell-specific isozyme of calmodulin (CaM)-dependent phosphodiesterase that exhibits micromolar affinity for cAMP has been purified 900-fold from mouse testis by DEAE chromatography, gel filtration, affinity chromatography with CaM-Sepharose 4B, and isoelectric focusing. Sepharose 233-242 calmodulin 2 Mus musculus 27-37 2844815-2 1988 A cell-specific isozyme of calmodulin (CaM)-dependent phosphodiesterase that exhibits micromolar affinity for cAMP has been purified 900-fold from mouse testis by DEAE chromatography, gel filtration, affinity chromatography with CaM-Sepharose 4B, and isoelectric focusing. Sepharose 233-242 calmodulin 2 Mus musculus 39-42 3217923-0 1988 Isolation of fibrinogen A alpha-chain by affinity chromatography on concanavalin A-sepharose. Sepharose 83-92 fibrinogen beta chain Homo sapiens 13-23 3217923-0 1988 Isolation of fibrinogen A alpha-chain by affinity chromatography on concanavalin A-sepharose. Sepharose 83-92 Fc gamma receptor and transporter Homo sapiens 26-37 3217923-2 1988 A mixture of the three constituent polypeptide chains of human fibrinogen was applied onto a column of concanavalin A-Sepharose. Sepharose 118-127 fibrinogen beta chain Homo sapiens 63-73 3170606-8 1988 Upon chromatography of the PC-stabilized enzyme on concanavalin A-agarose almost complete retention occurred at 0.4% Nonidet P-40, while no binding took place at a detergent concentration of 0.075%; this suggested that upon dilution in the presence of PC, the oligosaccharyltransferase was reconstituted into vesicles in an asymmetric fashion with its N-linked carbohydrate located internally. Sepharose 66-73 interleukin 9 Homo sapiens 125-129 3139669-4 1988 Fractionation of the resulting 35S-labeled octasaccharide on antithrombin-Sepharose yielded a high affinity fraction that accounted for approximately 2% of the total incorporated label. Sepharose 74-83 serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Mus musculus 61-73 3139669-10 1988 The 35S-labeled pentasaccharide recovered after incubation bound with high affinity to antithrombin-Sepharose and contained a 3-O-[35S]sulfate group at the internal glucosamine residue as the only detectable labeled component. Sepharose 100-109 serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Mus musculus 87-99 3178220-6 1988 We interpret these results as evidence that the stronger binding of fibronectin to gelatin-agarose in the presence of heparin is due to heparin itself binding to gelatin, thus allowing fibronectin to bind simultaneously to both immobilized ligands through appropriate domains of the glycoprotein. Sepharose 91-98 fibronectin 1 Homo sapiens 68-79 3170576-3 1988 The major scu-PA form was purified to homogeneity by ion-exchange chromatography on CM-Sephadex C-50, immunoadsorption on purified anti-u-PA IgG-Sepharose and affinity chromatography on p-amino-benzamidine-Agarose. Sepharose 145-154 plasminogen activator, urokinase Homo sapiens 12-16 3178841-3 1988 Affinity chromatography on glutathione-Sepharose and assays of enzyme activity indicate that MeAP29 is a member of the glutathione S-transferase family. Sepharose 39-48 hematopoietic prostaglandin D synthase Rattus norvegicus 119-144 3178220-2 1988 We observed, however, that the elution of purified human plasma fibronectin from heparin-treated gelatin-agarose required the same high urea concentrations regardless of whether heparin treatment preceded or followed fibronectin adsorption. Sepharose 105-112 fibronectin 1 Homo sapiens 64-75 3178220-6 1988 We interpret these results as evidence that the stronger binding of fibronectin to gelatin-agarose in the presence of heparin is due to heparin itself binding to gelatin, thus allowing fibronectin to bind simultaneously to both immobilized ligands through appropriate domains of the glycoprotein. Sepharose 91-98 fibronectin 1 Homo sapiens 185-196 3416817-4 1988 Agarose gel electrophoresis of total RNA extracts of RINr cells, followed by hybridization with v-myc DNA yielded a 2.4 kilobase c-myc messenger RNA (mRNA) transcripts. Sepharose 0-7 MYC proto-oncogene, bHLH transcription factor Rattus norvegicus 129-134 3143373-3 1988 TPA was isolated from the conditioned media using metal chelate affinity chromatography followed by immunoaffinity purification using mouse monoclonal anti-human TPA coupled to Sepharose. Sepharose 177-186 chromosome 20 open reading frame 181 Homo sapiens 0-3 3198249-2 1988 The mucin was purified by Sepharose 4B and 2B column chromatography. Sepharose 26-35 LOC100508689 Homo sapiens 4-9 2853710-5 1988 From the Triton X-extract of platelets, thrombomodulin was partially purified by diisopropylphosphoryl-thrombin-agarose affinity chromatography. Sepharose 112-119 thrombomodulin Homo sapiens 40-54 2853710-5 1988 From the Triton X-extract of platelets, thrombomodulin was partially purified by diisopropylphosphoryl-thrombin-agarose affinity chromatography. Sepharose 112-119 coagulation factor II, thrombin Homo sapiens 103-111 3417681-6 1988 Thrombin attached to CNBr-activated Sepharose through its amino groups did not bind to fragment E, but when thrombin was attached through its carboxyl groups, it bound fragment E. Sepharose 36-45 coagulation factor II, thrombin Homo sapiens 0-8 3178799-4 1988 The molecule has been identified as a bFGF-like molecule on the basis of its biological activity, its affinity for heparin-Sepharose, and its cross-reactivity with anti-human bFGF antibodies. Sepharose 123-132 fibroblast growth factor 2 Rattus norvegicus 38-42 3137226-2 1988 Insulin receptors that have been purified by wheat germ lectin chromatography and either tyrosine-agarose chromatography, sucrose density centrifugation, or insulin-Sepharose chromatography have been co-inserted into phospholipid vesicles with different purified G proteins. Sepharose 98-105 insulin Bos taurus 0-7 3178799-7 1988 On the basis of its affinity for heparin-Sepharose and its immunological characteristics, this protein appears to be an high molecular weight form of bFGF. Sepharose 41-50 fibroblast growth factor 2 Rattus norvegicus 150-154 3047122-8 1988 About 30% of human transferrin receptors made in insect cells do not form intermolecular disulfide bonds, but are recognized by the anti-transferrin receptor antibody, B3/25, and bind specifically to a human transferrin-Sepharose column. Sepharose 220-229 transferrin Homo sapiens 19-30 3047122-8 1988 About 30% of human transferrin receptors made in insect cells do not form intermolecular disulfide bonds, but are recognized by the anti-transferrin receptor antibody, B3/25, and bind specifically to a human transferrin-Sepharose column. Sepharose 220-229 transferrin Homo sapiens 137-148 3047122-8 1988 About 30% of human transferrin receptors made in insect cells do not form intermolecular disulfide bonds, but are recognized by the anti-transferrin receptor antibody, B3/25, and bind specifically to a human transferrin-Sepharose column. Sepharose 220-229 transferrin Homo sapiens 137-148 3137226-2 1988 Insulin receptors that have been purified by wheat germ lectin chromatography and either tyrosine-agarose chromatography, sucrose density centrifugation, or insulin-Sepharose chromatography have been co-inserted into phospholipid vesicles with different purified G proteins. Sepharose 165-174 insulin Bos taurus 0-7 3137226-2 1988 Insulin receptors that have been purified by wheat germ lectin chromatography and either tyrosine-agarose chromatography, sucrose density centrifugation, or insulin-Sepharose chromatography have been co-inserted into phospholipid vesicles with different purified G proteins. Sepharose 165-174 insulin Bos taurus 157-164 2460147-4 1988 A PAPP-A-free washing of the heparin-Sepharose column used during the purification of PAPP-A showed inhibitory activities similar to those of purified PAPP-A. Sepharose 37-46 pappalysin 1 Homo sapiens 86-92 2971097-6 1988 Marked enrichment in 5-HT1A binding sites relative to other soluble proteins was found in the peak fractions eluted from Sephacryl S-400 (by sixfold) and WGA-agarose (by 26-fold) columns, suggesting that these chromatographic steps might be of interest for the purification of central 5-HT1A receptors. Sepharose 158-165 5-hydroxytryptamine receptor 1A Rattus norvegicus 21-27 2460147-4 1988 A PAPP-A-free washing of the heparin-Sepharose column used during the purification of PAPP-A showed inhibitory activities similar to those of purified PAPP-A. Sepharose 37-46 pappalysin 1 Homo sapiens 86-92 2900677-4 1988 In the present study, we have further studied the properties of the P-glycoprotein ATPase activity by an immobilized enzyme assay procedure using a P-glycoprotein-antibody-Protein A-Sepharose complex. Sepharose 182-191 ATP binding cassette subfamily B member 1 Homo sapiens 68-82 2900677-4 1988 In the present study, we have further studied the properties of the P-glycoprotein ATPase activity by an immobilized enzyme assay procedure using a P-glycoprotein-antibody-Protein A-Sepharose complex. Sepharose 182-191 ATP binding cassette subfamily B member 1 Homo sapiens 148-162 2900689-0 1988 Crosslinking CD3 with CD2 using sepharose-immobilized antibodies enhances T lymphocyte proliferation. Sepharose 32-41 CD2 molecule Homo sapiens 22-25 2900689-3 1988 When CD3 was simultaneously crosslinked with CD2 using Sepharose beads coupled to antibodies directed at both determinants, T cell proliferation was markedly enhanced (stimulation index = 8- to 11-fold). Sepharose 55-64 CD2 molecule Homo sapiens 45-48 2900689-8 1988 Human thymocytes, the majority of which express both CD3 and CD2, were similarly activated by Sepharose-immobilized antibodies. Sepharose 94-103 CD2 molecule Homo sapiens 61-64 3409328-2 1988 The present studies show that PF4 prepared from normal mouse or human serum by absorption to heparin-agarose and elution between 0.5 and 1.5 M NaCl is also active in this respect. Sepharose 101-108 platelet factor 4 Mus musculus 30-33 2900773-4 1988 It is shown in vitro by immunohistochemistry, catalytic histochemistry, and by affinity chromatography of solubilized plasma membrane on collagen-Sepharose that DPP IV has a binding affinity to collagen. Sepharose 146-155 dipeptidylpeptidase 4 Rattus norvegicus 161-167 3170988-5 1988 IgG (+IgA) antibody was determined after absorption of the serum with anti-IgE Sepharose gel. Sepharose 79-88 CD79a molecule Homo sapiens 6-9 3417781-3 1988 The inhibitory effect of the immunoglobulins is dependent upon antibody concentration, is reversible, is overcome by the addition of recombinant bFGF, and is removed by affinity chromatography of the antiserum through a column of bFGF-Sepharose. Sepharose 235-244 fibroblast growth factor 2 Bos taurus 145-149 3417781-3 1988 The inhibitory effect of the immunoglobulins is dependent upon antibody concentration, is reversible, is overcome by the addition of recombinant bFGF, and is removed by affinity chromatography of the antiserum through a column of bFGF-Sepharose. Sepharose 235-244 fibroblast growth factor 2 Bos taurus 230-234 2842398-11 1988 C3d binding specificities contained in Ab2 were isolated by affinity chromatography on C3b/C3bi-Sepharose. Sepharose 96-105 endogenous retrovirus group K member 13 Homo sapiens 0-3 3187951-4 1988 The propositus" AT III was coeluted with normal AT III from an heparin-sepharose column. Sepharose 71-80 serpin family C member 1 Homo sapiens 16-22 3046780-0 1988 Improved agarose electrophoretic method for separating alkaline phosphatase isoenzymes in serum. Sepharose 9-16 alkaline phosphatase, placental Homo sapiens 55-75 3046780-1 1988 A modified agarose electrophoretic system for the separation of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes is described. Sepharose 11-18 alkaline phosphatase, placental Homo sapiens 64-84 3046780-1 1988 A modified agarose electrophoretic system for the separation of alkaline phosphatase (ALP, EC 3.1.3.1) isoenzymes is described. Sepharose 11-18 alkaline phosphatase, placental Homo sapiens 86-89 3046780-3 1988 The sensitivity of the agarose system is superior to cellulose acetate in detecting high-Mr ALP. Sepharose 23-30 alkaline phosphatase, placental Homo sapiens 92-95 3211159-2 1988 In the presence of calcium, SAP binds to certain complex polysaccharides, including agarose and zymosan. Sepharose 84-91 amyloid P component, serum Homo sapiens 28-31 3211159-3 1988 While the binding of SAP to agarose involves interaction with a galactose pyruvate acetal, the ligand in zymosan has not been defined. Sepharose 28-35 amyloid P component, serum Homo sapiens 21-24 3206560-3 1988 The specific activity of hepatic lipase purified from the ordinary heparin-containing homogenate by affinity chromatography on heparin-sepharose 4B was equal to 3800 microM of FFA/h per mg of protein. Sepharose 135-144 hepatic triacylglycerol lipase Oryctolagus cuniculus 25-39 2843101-7 1988 The Ah receptor contained in hamster hepatic cytosol underwent a ligand-dependent transformation in vitro to two forms having affinity for DNA-Sepharose, one of which was isolated from nuclei of animals treated with [3H]TCDD in vivo. Sepharose 143-152 aryl hydrocarbon receptor Mesocricetus auratus 4-15 2969895-6 1988 IL1-R was purified to apparent homogeneity from solubilized NOB-1 membranes by affinity chromatography on wheat germ agglutinin-Sepharose and IL1 alpha-Sepharose. Sepharose 128-137 interleukin 1 complex Mus musculus 0-3 3403561-8 1988 Fibronectin was isolated from citrated human plasma by sequential gelatin-Sepharose affinity and DEAE ion-exchange chromatography in the presence of buffers containing 1 mM phenylmethylsulfonyl fluoride to prevent fragmentation. Sepharose 74-83 fibronectin 1 Homo sapiens 0-11 3191112-9 1988 When fibrinogen is chromatographed on a column of agarose-bound B beta 15-42, a cation-dependent retention of fibrinogen on the peptide column was observed, and fibrinogen was eluted from the column by B beta 15-42 but not by B beta 1-14. Sepharose 50-57 fibrinogen beta chain Homo sapiens 5-15 3261688-1 1988 Human plasminogen activator inhibitor-1 (PAI-1) was purified from the conditioned medium of endotoxin-stimulated umbilical vein endothelial cell cultures by combinations of zinc-chelate-Sepharose chromatography, gel filtration on Sephacryl S-300 and immunoadsorption on an insolubilized murine monoclonal antibody (MA-7D4). Sepharose 186-195 serpin family E member 1 Homo sapiens 6-39 3261961-2 1988 In this paper we describe the purification of PABA-peptide hydrolase (PPH) by immunoaffinity chromatography using a monoclonal antibody (Mab), HBB 3/716/36, bound to protein A-Sepharose, and the characterization of the purified enzyme. Sepharose 176-185 meprin A subunit alpha Homo sapiens 46-68 3261961-2 1988 In this paper we describe the purification of PABA-peptide hydrolase (PPH) by immunoaffinity chromatography using a monoclonal antibody (Mab), HBB 3/716/36, bound to protein A-Sepharose, and the characterization of the purified enzyme. Sepharose 176-185 meprin A subunit alpha Homo sapiens 70-73 2839510-1 1988 Hepatic triglyceride lipase (H-TGL) was isolated from human postheparin plasma by column chromatography on heparin-Sepharose and phenyl-Sepharose and immunoaffinity chromatography with monoclonal antibodies. Sepharose 115-124 lipase C, hepatic type Homo sapiens 0-27 2839510-1 1988 Hepatic triglyceride lipase (H-TGL) was isolated from human postheparin plasma by column chromatography on heparin-Sepharose and phenyl-Sepharose and immunoaffinity chromatography with monoclonal antibodies. Sepharose 115-124 lipase C, hepatic type Homo sapiens 29-34 3191112-9 1988 When fibrinogen is chromatographed on a column of agarose-bound B beta 15-42, a cation-dependent retention of fibrinogen on the peptide column was observed, and fibrinogen was eluted from the column by B beta 15-42 but not by B beta 1-14. Sepharose 50-57 fibrinogen beta chain Homo sapiens 110-120 3191112-9 1988 When fibrinogen is chromatographed on a column of agarose-bound B beta 15-42, a cation-dependent retention of fibrinogen on the peptide column was observed, and fibrinogen was eluted from the column by B beta 15-42 but not by B beta 1-14. Sepharose 50-57 fibrinogen beta chain Homo sapiens 110-120 3191114-10 1988 Patient antithrombin III, isolated by affinity chromatography on heparin-Sepharose, was reacted with purified thrombin. Sepharose 73-82 serpin family C member 1 Homo sapiens 8-24 3191114-10 1988 Patient antithrombin III, isolated by affinity chromatography on heparin-Sepharose, was reacted with purified thrombin. Sepharose 73-82 coagulation factor II, thrombin Homo sapiens 12-20 3394658-0 1988 Agarose gel patterns of alkaline phosphatase isoenzymes before and after treatment with neuraminidase. Sepharose 0-7 neuraminidase 1 Homo sapiens 88-101 2456204-1 1988 A small form of hCG (SP-hCG) was purified from an acetone powder preparation of human first trimester placenta by repeated gel filtration and ion exchange chromatography on a Q-Sepharose or FPLC Mono Q column. Sepharose 177-186 chorionic gonadotropin subunit beta 5 Homo sapiens 16-19 3178740-11 1988 Inhibition of extracellular calmodulin activity by calmodulin antagonist immobilized on agarose beads, or by antibody to calmodulin, significantly decreased DNA synthesis. Sepharose 88-95 calmodulin 1 Homo sapiens 28-38 3178740-11 1988 Inhibition of extracellular calmodulin activity by calmodulin antagonist immobilized on agarose beads, or by antibody to calmodulin, significantly decreased DNA synthesis. Sepharose 88-95 calmodulin 1 Homo sapiens 51-61 3178740-11 1988 Inhibition of extracellular calmodulin activity by calmodulin antagonist immobilized on agarose beads, or by antibody to calmodulin, significantly decreased DNA synthesis. Sepharose 88-95 calmodulin 1 Homo sapiens 51-61 2458152-9 1988 Both non-disulfide-bonded and disulfide-bonded vitronectin bound to antibody-Sepharose from a mixture of vitronectin and thrombin-antithrombin III. Sepharose 77-86 vitronectin Homo sapiens 47-58 2458152-9 1988 Both non-disulfide-bonded and disulfide-bonded vitronectin bound to antibody-Sepharose from a mixture of vitronectin and thrombin-antithrombin III. Sepharose 77-86 vitronectin Homo sapiens 105-116 2458152-9 1988 Both non-disulfide-bonded and disulfide-bonded vitronectin bound to antibody-Sepharose from a mixture of vitronectin and thrombin-antithrombin III. Sepharose 77-86 coagulation factor II, thrombin Homo sapiens 121-129 2460147-4 1988 A PAPP-A-free washing of the heparin-Sepharose column used during the purification of PAPP-A showed inhibitory activities similar to those of purified PAPP-A. Sepharose 37-46 pappalysin 1 Homo sapiens 2-8 2460263-5 1988 The activated vitronectin specifically bound to heparin-Sepharose in 8 M urea and was eluted with 0.5 M NaCl containing 8 M urea. Sepharose 56-65 vitronectin Homo sapiens 14-25 3402455-8 1988 Iodinated osteonectin, previously incubated with purified thrombospondin, specifically bound to an anti-thrombospondin monoclonal antibody (P10) linked to protein-A--Sepharose 4B. Sepharose 166-175 secreted protein acidic and cysteine rich Bos taurus 10-21 3234379-0 1988 Subtyping of orosomucoid 1 (ORM1) by isoelectric focusing in agarose and polyacrylamide gels. Sepharose 61-68 orosomucoid 1 Homo sapiens 13-26 3234379-0 1988 Subtyping of orosomucoid 1 (ORM1) by isoelectric focusing in agarose and polyacrylamide gels. Sepharose 61-68 orosomucoid 1 Homo sapiens 28-32 3234386-1 1988 The genetic variants of the coagulation factor XIIIB (FXIIIB) were analyzed by isoelectric focusing, carried out in agarose gels and followed by immunofixation. Sepharose 116-123 coagulation factor XIII B chain Homo sapiens 40-52 3234386-1 1988 The genetic variants of the coagulation factor XIIIB (FXIIIB) were analyzed by isoelectric focusing, carried out in agarose gels and followed by immunofixation. Sepharose 116-123 coagulation factor XIII B chain Homo sapiens 54-60 3234387-0 1988 Two new esterase D (ESD) variants revealed by isoelectric focusing in agarose gel. Sepharose 70-77 esterase D Homo sapiens 8-18 3141158-2 1988 The lipase itself was strongly chemotactic; in addition preincubation of granulocytes with low concentrations of lipase enhanced the directed movement, as assayed in the agarose system. Sepharose 170-177 lipase Staphylococcus aureus 4-10 3141158-2 1988 The lipase itself was strongly chemotactic; in addition preincubation of granulocytes with low concentrations of lipase enhanced the directed movement, as assayed in the agarose system. Sepharose 170-177 lipase Staphylococcus aureus 113-119 2842235-7 1988 Cytosolic retinoic acid binding protein was separated at an isoelectric point of 4.5 on agarose gel. Sepharose 88-95 cellular retinoic acid binding protein 1 Homo sapiens 0-39 3234387-0 1988 Two new esterase D (ESD) variants revealed by isoelectric focusing in agarose gel. Sepharose 70-77 esterase D Homo sapiens 20-23 3402455-8 1988 Iodinated osteonectin, previously incubated with purified thrombospondin, specifically bound to an anti-thrombospondin monoclonal antibody (P10) linked to protein-A--Sepharose 4B. Sepharose 166-175 S100 calcium binding protein A10 Homo sapiens 140-143 3234387-1 1988 Using isoelectric focusing in thin-layer agarose gel (AGIF) with the narrow pH range of 4.5-5.4, a high resolution of esterase D (ESD) isozyme banding patterns has been achieved. Sepharose 41-48 interleukin 11 Homo sapiens 54-58 3234387-1 1988 Using isoelectric focusing in thin-layer agarose gel (AGIF) with the narrow pH range of 4.5-5.4, a high resolution of esterase D (ESD) isozyme banding patterns has been achieved. Sepharose 41-48 esterase D Homo sapiens 118-128 2969921-1 1988 Previous investigations of p150,95 (CD11c), the third member of the CD18 membrane glycoprotein family that includes CR3 (Mac-1 or CD11b) and LFA-1 (CD11a), had demonstrated that solubilized p150,95 bound to iC3b-agarose in a manner similar to isolated CR3. Sepharose 212-219 integrin subunit alpha X Homo sapiens 36-41 3234387-1 1988 Using isoelectric focusing in thin-layer agarose gel (AGIF) with the narrow pH range of 4.5-5.4, a high resolution of esterase D (ESD) isozyme banding patterns has been achieved. Sepharose 41-48 esterase D Homo sapiens 130-133 3134521-3 1988 Vascular permeability factor activity falls into a molecular weight range of 41,000 to 56,000 D. Activity is bound to hydroxylapatite, carboxymethyl-Sepharose, phenyl-Sepharose, and heparin-Sepharose, whereas little or no activity was bound to diethylaminoethyl-Sephacel. Sepharose 149-158 vascular endothelial growth factor A Homo sapiens 0-28 2455774-6 1988 When the serum was purified by affinity chromatography on a myelin basic protein-conjugated Sepharose column the nonadsorbed material lost this activity whereas the eluted antibodies reacted with myelin basic protein and synapsin. Sepharose 92-101 myelin basic protein Rattus norvegicus 60-80 2840071-3 1988 Rechromatography of peak GII on heparin-agarose, in the presence of 0.5% sodium cholate, resulted in separation of PLC and GTP-binding activities, and loss of GTP-dependent PLC activity. Sepharose 40-47 heparan sulfate proteoglycan 2 Homo sapiens 115-118 3392021-9 1988 A 3H-labeled single component was purified from the digests by sequential chromatography through anti-ALP-IgG-Sepharose, concanavalin A-Sepharose, Bio-Gel P-6, and TSK G-2000 columns. Sepharose 110-119 alkaline phosphatase, placental Homo sapiens 102-105 2967821-5 1988 Bovine liver Man-6-P receptor, prepared by the conventional method of affinity chromatography on phosphomannan-Sepharose, bound IGF-II with high affinity (Kd = 1 nM). Sepharose 111-120 insulin like growth factor 2 Homo sapiens 128-134 3140779-1 1988 The low density lipoprotein (LDL) receptor has been purified to homogeneity from rabbit liver by a combination of DEAE-Sephacel chromatography, LDL-Sepharose 4B chromatography and preparative SDS/polyacrylamide-gel electrophoresis. Sepharose 148-157 low-density lipoprotein receptor Oryctolagus cuniculus 4-42 3384824-2 1988 Lysophospholipase I was purified (8,500-fold) to homogeneity by DEAE-Sephacel, Sephadex G-75, Blue-Sepharose, and chromatofocusing chromatography. Sepharose 99-108 lysophospholipase 1 Homo sapiens 0-19 3384824-3 1988 Lysophospholipase II was separated from the lysophospholipase I in the Blue-Sepharose step. Sepharose 76-85 lysophospholipase 2 Homo sapiens 0-20 3384824-3 1988 Lysophospholipase II was separated from the lysophospholipase I in the Blue-Sepharose step. Sepharose 76-85 lysophospholipase 1 Homo sapiens 44-63 3395272-5 1988 Nine apo B-100 hydrophilic peptides, 12 to 26 amino acids long, were selected, and three were found to interact with the agarose-bound CSPG: apo B P-1 (LRKHKLIDVISMYRELLKDLSKEA, residues 4230 to 4254), apo B P-2 (RLTRKRGLKLATALSLSNK, residues 3359 to 3377), and apo B P-11 (RQVSHAKEKLTALTKK, residues 2106 to 2121). Sepharose 121-128 apolipoprotein B Homo sapiens 5-14 3395272-5 1988 Nine apo B-100 hydrophilic peptides, 12 to 26 amino acids long, were selected, and three were found to interact with the agarose-bound CSPG: apo B P-1 (LRKHKLIDVISMYRELLKDLSKEA, residues 4230 to 4254), apo B P-2 (RLTRKRGLKLATALSLSNK, residues 3359 to 3377), and apo B P-11 (RQVSHAKEKLTALTKK, residues 2106 to 2121). Sepharose 121-128 crystallin gamma F, pseudogene Homo sapiens 147-150 3395272-5 1988 Nine apo B-100 hydrophilic peptides, 12 to 26 amino acids long, were selected, and three were found to interact with the agarose-bound CSPG: apo B P-1 (LRKHKLIDVISMYRELLKDLSKEA, residues 4230 to 4254), apo B P-2 (RLTRKRGLKLATALSLSNK, residues 3359 to 3377), and apo B P-11 (RQVSHAKEKLTALTKK, residues 2106 to 2121). Sepharose 121-128 S100 calcium binding protein A10 Homo sapiens 268-272 3395272-10 1988 Apo E also binds to the agarose-proteoglycan. Sepharose 24-31 apolipoprotein E Homo sapiens 0-5 3255355-2 1988 The polypeptide fractions separated from the heat-inactivated dialyzed plasma using streptavidin-Sepharose resin showed the same effect on purified biotinidase. Sepharose 97-106 biotinidase Homo sapiens 148-159 3378206-2 1988 The present study demonstrates that PKC binds specifically and reversibly to the antiestrogen N-didesmethyltamoxifen when the drug is coupled to CNBr-activated agarose through its primary amine, in the absence of lipid and other cofactors of the enzyme. Sepharose 160-167 proline rich transmembrane protein 2 Homo sapiens 36-39 3378206-3 1988 PKC did not bind to 4-hydroxytamoxifen, which had been immobilized on epoxy-activated Sepharose through its hydroxyl moiety. Sepharose 86-95 proline rich transmembrane protein 2 Homo sapiens 0-3 3137155-1 1988 In the model system used here, cross-linking of T-cell receptor structures (TCR) by antigen-presenting cells (APCs) is substituted by the use of anti-F23.1 anti-T-cell receptor monoclonal antibody immobilized on Sepharose beads. Sepharose 212-221 T cell receptor alpha variable 6-3 Mus musculus 76-79 2838349-2 1988 Further evidence of the similarity of this growth factor to FGF is provided by the finding that biological activity is lost when the material is bound to a heparin-Sepharose column and restored upon elution with 2.5 M NaCl; the 2.5 M NaCl fraction from Day 12 embryos contains several polypeptides of apparent molecular weights 12,500-17,500. Sepharose 164-173 fibroblast growth factor 10 Gallus gallus 60-63 2842255-4 1988 In contrast, neutrophils preincubated with rH GM-CSF exhibited reduced chemotaxis under agarose in response to a gradient of f Met-Leu-Phe. Sepharose 88-95 colony stimulating factor 2 Homo sapiens 46-52 3392521-7 1988 Addition of 2-aminopurine to an agarose overlay resulted in high efficiency plaque formation by vesicular stomatitis virus New Jersey (Hazelhurst) under conditions where endogenous induction of interferon and its feedback action on aged chick embryo cells normally prevented plaque formation. Sepharose 32-39 interferon Gallus gallus 194-204 2839770-1 1988 Thymidylate synthase-negative mutant mouse cells starved of thymidine or their parental FM3A cells treated with 5-fluoro-2"-deoxyuridine produced DNA fragments ranging from 50 to 200 kilobase pairs with a peak at 100 kb in length as determined by pulsed-field agarose gel electrophoresis. Sepharose 260-267 thymidylate synthase Mus musculus 0-20 3412326-1 1988 A human astrocytoma cell line, U87-MG, synthesizes a growth factor which is structurally related to basic fibroblast growth factor (bFGF) by several criteria: 1) it binds to heparin-Sepharose and elutes at 2 M NaCl; 2) it cross-reacts with N-terminal specific anti-bFGF antibodies; 3) it is a potent mitogen for rabbit fetal chondrocytes. Sepharose 182-191 fibroblast growth factor 2 Homo sapiens 100-130 3412326-1 1988 A human astrocytoma cell line, U87-MG, synthesizes a growth factor which is structurally related to basic fibroblast growth factor (bFGF) by several criteria: 1) it binds to heparin-Sepharose and elutes at 2 M NaCl; 2) it cross-reacts with N-terminal specific anti-bFGF antibodies; 3) it is a potent mitogen for rabbit fetal chondrocytes. Sepharose 182-191 fibroblast growth factor 2 Homo sapiens 132-136 3418194-4 1988 The affinity of PR for ATP was evaluated on ATP-Sepharose column chromatography. Sepharose 48-57 progesterone receptor Homo sapiens 16-18 2455298-3 1988 Two protocols were followed to purify the IFN-gamma receptor from octyl glucoside-solubilized membranes: (i) sequential affinity chromatography over wheat germ agglutinin- and IFN-gamma-Sepharose and (ii) affinity chromatography over columns containing receptor-specific monoclonal antibody and wheat germ agglutinin. Sepharose 186-195 interferon gamma Homo sapiens 42-51 2455298-3 1988 Two protocols were followed to purify the IFN-gamma receptor from octyl glucoside-solubilized membranes: (i) sequential affinity chromatography over wheat germ agglutinin- and IFN-gamma-Sepharose and (ii) affinity chromatography over columns containing receptor-specific monoclonal antibody and wheat germ agglutinin. Sepharose 186-195 interferon gamma Homo sapiens 176-185 3188251-0 1988 [Affinity chromatography of human gamma-thrombin on fibrin-agarose]. Sepharose 59-66 coagulation factor II, thrombin Homo sapiens 40-48 3188251-5 1988 Fibrin-agarose possessing affinity to gamma-thrombin and specifically not binding gamma-thrombin was used to remove admixtures of the coagulant gamma-thrombin from the preparations of gamma-thrombin obtained during the enzyme autolysis. Sepharose 7-14 coagulation factor II, thrombin Homo sapiens 44-52 3195134-4 1988 The precipitated neuraminidase from human chorion consisted of two forms, bound- and unbound with Con A-Sepharose. Sepharose 104-113 neuraminidase 1 Homo sapiens 17-30 3413737-4 1988 Two populations of ATIII were detected by affinity chromatography on heparin-sepharose from affected members" plasma. Sepharose 77-86 serpin family C member 1 Homo sapiens 19-24 3413737-5 1988 The ATIII unbound to sepharose beads was devoid of heparin cofactor activity and showed a lack of anodal migration in CIE in the presence of heparin. Sepharose 21-30 serpin family C member 1 Homo sapiens 4-9 3288621-4 1988 The recombinant PF4 (rPF4) was purified by heparin-agarose affinity chromatography and reverse-phase high performance liquid chromatography. Sepharose 51-58 platelet factor 4 Homo sapiens 16-19 3288621-4 1988 The recombinant PF4 (rPF4) was purified by heparin-agarose affinity chromatography and reverse-phase high performance liquid chromatography. Sepharose 51-58 platelet factor 4 Rattus norvegicus 21-25 3187930-3 1988 The method was based upon affinity chromatography utilizing the high affinity binding of tissue factor to Factor VII noncovalently complexed to immobilized anti-Factor VII-agarose beads. Sepharose 172-179 coagulation factor III, tissue factor Homo sapiens 89-102 3187930-4 1988 The apparent molecular weight of the purified tissue factor is 45,000 as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and its isoelectric point is 4.8-5.1 by column chromatofocussing and flat bed agarose isoelectric focussing. Sepharose 220-227 coagulation factor III, tissue factor Homo sapiens 46-59 2836066-2 1988 The trans-acting factors were purified from mouse myeloma NS1 cells, and HIG1-inducing activity was found mainly in fractions of molecular weight 53-127 kd and in a fraction eluted from a heparin-Sepharose column with 0.5 M KCI. Sepharose 196-205 HIG1 domain family, member 1A Mus musculus 73-77 2967288-2 1988 In this study a series of proteolytic fragments of neuromodulin were systematically screened for calmodulin-Sepharose binding activity. Sepharose 108-117 growth associated protein 43 Homo sapiens 51-63 2967288-2 1988 In this study a series of proteolytic fragments of neuromodulin were systematically screened for calmodulin-Sepharose binding activity. Sepharose 108-117 calmodulin 1 Homo sapiens 97-107 2897800-7 1988 During the suckling period, IF bound well to the ileal receptor, despite lower than adult levels of activity and different glycosylation, as assessed by binding to concanavalin A-Sepharose beads. Sepharose 179-188 cobalamin binding intrinsic factor Rattus norvegicus 28-30 3214414-5 1988 Using a modified agarose isoelectric focusing procedure, gastric ALDH I, ALDH II, and up to five ALDH III forms could be clearly resolved. Sepharose 17-24 aldehyde dehydrogenase 2 family member Homo sapiens 65-71 3214414-5 1988 Using a modified agarose isoelectric focusing procedure, gastric ALDH I, ALDH II, and up to five ALDH III forms could be clearly resolved. Sepharose 17-24 aldehyde dehydrogenase 3 family member A1 Homo sapiens 97-105 2455685-4 1988 UCHL1-coupled Sepharose beads were used to absorb antigen from lysates of cell lines. Sepharose 14-23 ubiquitin C-terminal hydrolase L1 Homo sapiens 0-5 2455685-6 1988 Antigen immobilized with CD45 mAb-coupled Sepharose beads bound UCHL1. Sepharose 42-51 protein tyrosine phosphatase receptor type C Homo sapiens 25-29 2455685-6 1988 Antigen immobilized with CD45 mAb-coupled Sepharose beads bound UCHL1. Sepharose 42-51 ubiquitin C-terminal hydrolase L1 Homo sapiens 64-69 3286501-9 1988 In addition, EAP exerted stimulatory activity on resting human T lymphocytes, costimulated with Sepharose-bound anti-CD3 monoclonal antibody 64.1, comparable to that observed with purified human monocyte IL-1. Sepharose 96-105 ribosomal protein L22 Homo sapiens 13-16 2458470-2 1988 The embryo-derived histamine-releasing factor (EHRF) has been isolated from the culture medium by means of heparin-Sepharose affinity chromatography. Sepharose 115-124 tumor protein, translationally-controlled 1 Homo sapiens 19-45 2843763-1 1988 The progesterone receptor (PR) was partially purified from T47D human breast cancer cells by sequential chromatography on phosphocellulose, heparin-Sepharose, and DNA-cellulose. Sepharose 148-157 progesterone receptor Homo sapiens 4-25 2843763-1 1988 The progesterone receptor (PR) was partially purified from T47D human breast cancer cells by sequential chromatography on phosphocellulose, heparin-Sepharose, and DNA-cellulose. Sepharose 148-157 progesterone receptor Homo sapiens 27-29 3366765-7 1988 Electrophoretic light scattering and the binding of type IV collagen by fibrinogen-Sepharose further establish the interaction between type IV collagen and fibrinogen. Sepharose 83-92 fibrinogen beta chain Homo sapiens 72-82 3366765-7 1988 Electrophoretic light scattering and the binding of type IV collagen by fibrinogen-Sepharose further establish the interaction between type IV collagen and fibrinogen. Sepharose 83-92 fibrinogen beta chain Homo sapiens 156-166 2834379-2 1988 Ceruloplasmin was isolated to purity from chicken plasma by a single-step chromatography on amino-ethyl-derivatized Sepharose. Sepharose 116-125 ceruloplasmin Gallus gallus 0-13 3421895-5 1988 Rat kidney cytosol was separated into three "affinity families" of GST activity after elution from a GSH-agarose matrix. Sepharose 105-112 hematopoietic prostaglandin D synthase Rattus norvegicus 67-70 2966154-6 1988 The Lp(a)-Sepharose column bound lipoproteins containing apolipoprotein B and other apolipoproteins. Sepharose 10-19 lipoprotein(a) Homo sapiens 4-9 2966154-6 1988 The Lp(a)-Sepharose column bound lipoproteins containing apolipoprotein B and other apolipoproteins. Sepharose 10-19 apolipoprotein B Homo sapiens 57-73 2966154-9 1988 Chemical modification of the lysine residues of apolipoprotein B (apoB) by either acetylation or acetoacetylation prevented or diminished the interaction of LDL2 with Lp(a), as shown by both agarose electrophoresis and ligand blotting using modified LDL2. Sepharose 191-198 apolipoprotein B Homo sapiens 48-64 2966154-9 1988 Chemical modification of the lysine residues of apolipoprotein B (apoB) by either acetylation or acetoacetylation prevented or diminished the interaction of LDL2 with Lp(a), as shown by both agarose electrophoresis and ligand blotting using modified LDL2. Sepharose 191-198 apolipoprotein B Homo sapiens 66-70 2899407-7 1988 Thus poly(NAT) gels are most suitable for the electrophoretic separation of DNA molecules whose size is out of the optimal fractionation range of poly(AA) or agarose gels. Sepharose 158-165 bromodomain containing 2 Homo sapiens 10-13 2835007-7 1988 The calmodulin-stimulated phosphodiesterase activity in the sperm plasma membranes can be solubilized and absorbed to a Calmodulin-Sepharose affinity column in the presence of Ca2+. Sepharose 131-140 calmodulin Bos taurus 4-14 2835007-7 1988 The calmodulin-stimulated phosphodiesterase activity in the sperm plasma membranes can be solubilized and absorbed to a Calmodulin-Sepharose affinity column in the presence of Ca2+. Sepharose 131-140 calmodulin Bos taurus 120-130 2458772-5 1988 Unlike the substrate, the EF-2 kinase had no affinity for RNA and therefore could be separated from EF-2 by chromatography on RNA-Sepharose. Sepharose 130-139 eukaryotic translation elongation factor 2 Homo sapiens 26-30 2458772-5 1988 Unlike the substrate, the EF-2 kinase had no affinity for RNA and therefore could be separated from EF-2 by chromatography on RNA-Sepharose. Sepharose 130-139 eukaryotic translation elongation factor 2 Homo sapiens 100-104 3202970-1 1988 Cathepsin D was affinity-purified on pepstatin-Sepharose from control rat liver, from Yoshida ascites hepatoma (AH-130) cells, and from the liver of AH-130 tumour-bearing rats. Sepharose 47-56 cathepsin D Rattus norvegicus 0-11 3202971-3 1988 A crude preparation of the acrosin inhibitor was prepared by immunoaffinity chromatography on anti-aprotinin antibodies bound to Sepharose 4B column. Sepharose 129-138 pancreatic trypsin inhibitor Bos taurus 99-108 18584668-0 1988 Reversible immobilization of soybean beta-amylase on phenylboronate-agarose. Sepharose 68-75 beta-amylase Glycine max 37-49 18584668-1 1988 Soybean beta-amylase (EC 3.2.1.2) wap immobilized on phenylboronate-agarose by strong interactive binding. Sepharose 68-75 beta-amylase Glycine max 8-20 3281751-7 1988 In soft agarose, both HCT 116 and HCT 116a subpopulations showed a stringent requirement for transferrin. Sepharose 8-15 transferrin Homo sapiens 93-104 3402860-1 1988 One hundred and ten pairs of blood and semen samples and their stains were studied to type glyoxalase 1 (GLO 1) isoenzymes using agarose-starch medium. Sepharose 129-136 glyoxalase I Homo sapiens 91-103 3402860-1 1988 One hundred and ten pairs of blood and semen samples and their stains were studied to type glyoxalase 1 (GLO 1) isoenzymes using agarose-starch medium. Sepharose 129-136 glyoxalase I Homo sapiens 105-110 2895745-8 1988 The CFA/I-specific receptor species also bound to wheat germ agglutinin-agarose. Sepharose 72-79 tubulin folding cofactor A Homo sapiens 4-7 3128478-7 1988 The lactoferrin-binding protein was identified as a 105,000-molecular-weight iron-regulated outer membrane protein in three different meningococcal strains by a batch affinity method with biotinylated human lactoferrin and streptavidin-agarose. Sepharose 236-243 lactotransferrin Bos taurus 4-15 2452833-3 1988 We have employed monoclonal antibody anti-T3B covalently coupled to CnBr-activated Sepharose 4B beads, to show that multimeric ligation of T cell antigen receptor leads to T cell receptiveness to interleukin 1 (IL-1), as indicated by T cell production of CSF, which induces growth of myeloid progenitor cells into neutrophil, eosinophil, and monocyte colonies. Sepharose 83-95 interleukin 1 alpha Homo sapiens 196-209 3367095-2 1988 In the course of analyzing 61 SS patients for their autoantibody profiles, we found that 42 were positive for anti-SS-A by double diffusion in agarose and demonstrated precipitin lines identical to that produced by a prototype anti-SS-A serum. Sepharose 143-150 tripartite motif containing 21 Homo sapiens 115-119 3396757-3 1988 MGSA has been purified to homogeneity from conditioned medium of Hs294T human malignant melanoma cells using acetic acid extraction of the crude conditioned medium followed by three chromatographic processes, including gel-filtration, heparin-Sepharose, and reverse-phase HPLC. Sepharose 243-252 C-X-C motif chemokine ligand 1 Homo sapiens 0-4 3396757-4 1988 MGSA was eluted from the heparin-Sepharose resin with 0.1-0.3 M NaCl. Sepharose 33-42 C-X-C motif chemokine ligand 1 Homo sapiens 0-4 3396757-8 1988 This bioactivity was progressively inactivated during storage at -80 degrees C. These results indicate that the combination of heparin-Sepharose chromatography and reverse phase-HPLC provides a more efficient means of purification of MGSA. Sepharose 135-144 C-X-C motif chemokine ligand 1 Homo sapiens 234-238 3174604-1 1988 NADP-hydrazidoadipoyl oxypropyl sepharose was synthesized from epoxyactivated sepharose through a hydrazid derivative and used for isolation of NADP(H)-dependent enzymes such as glutathione reductase, isocitrate dehydrogenase and malate dehydrogenase. Sepharose 32-41 glutathione-disulfide reductase Rattus norvegicus 178-199 2834234-1 1988 The duplex DNA unwinding ability of seminalplasmin [corrected] from bovine semen was examined by treatment of plasmid-protein complexes with calf thymus topoisomerase I and resolution of the topoisomer distributions by agarose gel electrophoresis. Sepharose 219-226 caltrin Bos taurus 36-50 3220661-5 1988 Affinity chromatographic analysis by an albumin-Sepharose column revealed that the enzyme samples labeled with SMA (SMA-SOD) tightly bound to the column, while unmodified SOD was eluted in the unbound fractions. Sepharose 48-57 survival of motor neuron 1, telomeric Homo sapiens 111-114 3220661-5 1988 Affinity chromatographic analysis by an albumin-Sepharose column revealed that the enzyme samples labeled with SMA (SMA-SOD) tightly bound to the column, while unmodified SOD was eluted in the unbound fractions. Sepharose 48-57 survival of motor neuron 1, telomeric Homo sapiens 116-123 3356702-3 1988 The half-life of the solubilized oxidoreductase stored at 2-4 degrees C in the presence of 25% glycerol at pH 8.6 is approximately 30 h. The oxidoreductase contains a flavoprotein identifiable by its fluorescence spectrum for FAD which binds weakly to concanavalin A-Sepharose and elutes from gel sieving columns at a molecular weight range of approximately 51,000. Sepharose 267-276 thioredoxin reductase 1 Homo sapiens 33-47 3356702-3 1988 The half-life of the solubilized oxidoreductase stored at 2-4 degrees C in the presence of 25% glycerol at pH 8.6 is approximately 30 h. The oxidoreductase contains a flavoprotein identifiable by its fluorescence spectrum for FAD which binds weakly to concanavalin A-Sepharose and elutes from gel sieving columns at a molecular weight range of approximately 51,000. Sepharose 267-276 thioredoxin reductase 1 Homo sapiens 141-155 3356702-5 1988 Recovery of oxidoreductase activity from both concanavalin A-Sepharose affinity and gel sieving columns is affected by the resolution of the flavoprotein free of the cytochrome b component of the oxidoreductase. Sepharose 61-70 thioredoxin reductase 1 Homo sapiens 12-26 3356702-5 1988 Recovery of oxidoreductase activity from both concanavalin A-Sepharose affinity and gel sieving columns is affected by the resolution of the flavoprotein free of the cytochrome b component of the oxidoreductase. Sepharose 61-70 mitochondrially encoded cytochrome b Homo sapiens 166-178 3356702-5 1988 Recovery of oxidoreductase activity from both concanavalin A-Sepharose affinity and gel sieving columns is affected by the resolution of the flavoprotein free of the cytochrome b component of the oxidoreductase. Sepharose 61-70 thioredoxin reductase 1 Homo sapiens 196-210 3356705-3 1988 The phospholipase A2 was solubilized from the 108,000 x g pellet fraction with 0.3% lithium dodecyl sulfate and then purified to homogeneity by successive DEAE-Cellulofine AM, octyl-Sepharose, Cellulofine GCL 300-m, S-Sepharose, and Bio-Gel P-30 chromatographies in the presence of 0.5% 3-[(3-cholamidopropyl)dimethylammonio]-1-propane-sulfonate. Sepharose 182-191 phospholipase A2 group IB Rattus norvegicus 4-20 3355847-5 1988 Furthermore, when postheparin plasma was mixed with monoclonal antibody prior to gel filtration on 6% agarose, the LPL activity eluted with the lipoproteins and was not inhibited by the antibody. Sepharose 102-109 lipoprotein lipase Homo sapiens 115-118 3360140-3 1988 Antithrombin Glasgow was purified from plasma using heparin-Sepharose chromatography at pH 7.4 eluting with increasing concentrations of NaCl. Sepharose 60-69 serpin family C member 1 Homo sapiens 0-12 2897886-0 1988 Agarose isoelectric focusing of arylsulfatase A producing a permanent record on slab gels. Sepharose 0-7 arylsulfatase A Homo sapiens 32-47 3258613-6 1988 Both Ag-specific GEF and nonspecific GEF from the hybridoma bind to p-aminobenzamidine-agarose, and are recovered by elution with benzamidine. Sepharose 87-94 rho/rac guanine nucleotide exchange factor (GEF) 2 Mus musculus 17-20 2455942-0 1988 Chromatography of the VIII/vWF complex on dextran sulphate sepharose. Sepharose 59-68 cytochrome c oxidase subunit 8A Homo sapiens 22-26 2455942-0 1988 Chromatography of the VIII/vWF complex on dextran sulphate sepharose. Sepharose 59-68 von Willebrand factor Homo sapiens 27-30 2455942-1 1988 The applicability of Dextran Sulphate (DS) agarose in the purification of the VIII/vWf complex from cryoprecipitate was investigated. Sepharose 43-50 cytochrome c oxidase subunit 8A Homo sapiens 78-82 3351312-1 1988 Mouse haptoglobin was isolated from acute-phase serum initially by affinity chromatography on haemoglobin-Sepharose. Sepharose 106-115 haptoglobin Mus musculus 6-17 3351312-4 1988 Subsequently, specific anti-haptoglobin antibodies were prepared by affinity chromatography on haptoglobin-Sepharose. Sepharose 107-116 haptoglobin Mus musculus 28-39 3351312-4 1988 Subsequently, specific anti-haptoglobin antibodies were prepared by affinity chromatography on haptoglobin-Sepharose. Sepharose 107-116 haptoglobin Mus musculus 95-106 2832415-4 1988 The isolated complex eluted from the gel filtration column exhibited an enhanced rate of ligand dissociation in response to GTP gamma S. Approximately 0.65 mol of pertussis toxin substrate/mol of receptor was estimated following partial purification of the receptor-ligand complex by sequential chromatography on wheat germ agglutinin-Sepharose, DEAE-Fractogel, and Ultrogel AcA 34. Sepharose 335-344 small nucleolar RNA, H/ACA box 2C Homo sapiens 375-381 2965148-3 1988 (a) By Sepharose exclusion chromatography the [35S]sulfate-labeled major HSPG had a nominal Mr of 200-250 X 10(3), consisting of a core protein of about 80-90 X 10(3) Mr with about 8-10 heparan sulfate glycosaminoglycan chains (Mr = 13 X 10(3)). Sepharose 7-16 syndecan 2 Mus musculus 73-77 3128176-1 1988 Plasminogen activator inhibitor 1 (PAI-1) was purified from medium conditioned by cultured bovine aortic endothelial cells by successive chromatography on concanavalin A Sepharose, Sephacryl S-200, Blue B agarose, and Bio-Gel P-60. Sepharose 170-179 serpin family E member 1 Bos taurus 0-33 3128176-1 1988 Plasminogen activator inhibitor 1 (PAI-1) was purified from medium conditioned by cultured bovine aortic endothelial cells by successive chromatography on concanavalin A Sepharose, Sephacryl S-200, Blue B agarose, and Bio-Gel P-60. Sepharose 170-179 serpin family E member 1 Bos taurus 35-40 3128176-1 1988 Plasminogen activator inhibitor 1 (PAI-1) was purified from medium conditioned by cultured bovine aortic endothelial cells by successive chromatography on concanavalin A Sepharose, Sephacryl S-200, Blue B agarose, and Bio-Gel P-60. Sepharose 205-212 serpin family E member 1 Bos taurus 0-33 3128176-1 1988 Plasminogen activator inhibitor 1 (PAI-1) was purified from medium conditioned by cultured bovine aortic endothelial cells by successive chromatography on concanavalin A Sepharose, Sephacryl S-200, Blue B agarose, and Bio-Gel P-60. Sepharose 205-212 serpin family E member 1 Bos taurus 35-40 3281588-5 1988 Further a monospecific polyclonal antibody to immunopurified GPIIIa coupled to protein A-Sepharose adsorbed GPIIIa but not aggregin. Sepharose 89-98 integrin subunit beta 3 Homo sapiens 61-67 3281588-5 1988 Further a monospecific polyclonal antibody to immunopurified GPIIIa coupled to protein A-Sepharose adsorbed GPIIIa but not aggregin. Sepharose 89-98 integrin subunit beta 3 Homo sapiens 108-114 3258613-6 1988 Both Ag-specific GEF and nonspecific GEF from the hybridoma bind to p-aminobenzamidine-agarose, and are recovered by elution with benzamidine. Sepharose 87-94 rho/rac guanine nucleotide exchange factor (GEF) 2 Mus musculus 37-40 3390155-1 1988 Hydroxymethylbilane synthase from human erythrocytes was purified 47,000-fold to greater than 95% homogeneity and 7.5% yield by a simple and rapid procedure using heat treatment (80 degrees C, in the presence of proteinase inhibitors, to convert one of two chromatographically separable forms into the other), DEAE-cellulose and Cibacron Blue F3G-A-Sepharose chromatographies and Sephadex G-75 gel filtration. Sepharose 349-358 hydroxymethylbilane synthase Homo sapiens 0-28 2463152-2 1988 Elution with 20 mM pyridoxal 5"-phosphate of the receptor from DNA Sepharose was more efficient than that with diluted pyridoxal 5"-phosphate, high ionic solution or various concentrations of Mg++, 3H-dihydrotestosterone bromoacetate was applicable to covalent binding with partially purified androgen receptor regardless of the low specificity of the ligand. Sepharose 67-76 androgen receptor Homo sapiens 293-310 3396587-4 1988 VSM cells contain 3.7 and 7.0 kilobase bFGF gene transcripts, which are translated into material closely related, if not identical, with bFGF when analyzed by its chromatographic behavior on heparin Sepharose, by immunoblot and by radioimmuno- and radioreceptor assays. Sepharose 199-208 fibroblast growth factor 2 Bos taurus 39-43 3154776-2 1988 A new electrophoretic technique for the determination of the glyoxalase I (GLO1) polymorphism on mixed agarose and starch gel is applied to the genetic study of "Provinces Francaises" and some other human populations. Sepharose 103-110 glyoxalase I Homo sapiens 61-73 3154776-2 1988 A new electrophoretic technique for the determination of the glyoxalase I (GLO1) polymorphism on mixed agarose and starch gel is applied to the genetic study of "Provinces Francaises" and some other human populations. Sepharose 103-110 glyoxalase I Homo sapiens 75-79 3390156-5 1988 The biological activity present in neutrophil supernatants co-purified with enzymic activity for cathepsin G during sequential Aprotinin-Sepharose affinity chromatography and carboxymethyl-Sephadex chromatography. Sepharose 137-146 cathepsin G Homo sapiens 97-108 3361951-3 1988 Thrombopoietin was retained by concanavalin-A-Sepharose. Sepharose 46-55 thrombopoietin Homo sapiens 0-14 3398860-12 1988 wt, agarose gel electrophoretic mobility and hydrophobicity was respectively: C5 greater than or equal to C5FI greater than C5FII greater than C5FIII; C5 less than or equal to C5FI less than or equal to C5FII much less than C5FIII; and C5FII greater than or equal to C5 greater than C5FI much greater than C5FIII. Sepharose 4-11 complement C5 Homo sapiens 78-80 2451242-4 1988 p115 from detergent-treated small cell lung carcinoma cells binds to bombesin-Sepharose and can be phosphorylated on tyrosine in the presence of radiolabeled ATP and Mn2+. Sepharose 78-87 USO1 vesicle transport factor Homo sapiens 0-4 2451242-4 1988 p115 from detergent-treated small cell lung carcinoma cells binds to bombesin-Sepharose and can be phosphorylated on tyrosine in the presence of radiolabeled ATP and Mn2+. Sepharose 78-87 gastrin releasing peptide Homo sapiens 69-77 3258254-0 1988 An amphibian two-domain "big" neurophysin: conformational homology with the mammalian MSEL-neurophysin/copeptin intermediate precursor shown by trypsin-sepharose proteolysis. Sepharose 152-161 arginine vasopressin Homo sapiens 103-111 3349091-5 1988 The identity of the lipase activity was demonstrated by specific inhibition with antisera to lipoprotein lipase, and to hepatic lipase, respectively, and by separation of the two lipase activities by chromatography on heparin-Sepharose. Sepharose 226-235 lipase G, endothelial type Rattus norvegicus 20-26 3378041-2 1988 Chromatography of various domains of the central nodule (desAB-NDSK, fibrinogen E, and fibrin E) having nonidentical amino acid sequences showed that all of these fragments are capable of binding to PMSF-thrombin-Sepharose, suggesting that the thrombin binding site resides within the peptide regions common to all of these fragments: alpha(Gly17-Met51), beta(Val55-Met118), and gamma(Tyr1-Lys53). Sepharose 213-222 coagulation factor II, thrombin Homo sapiens 204-212 3378041-4 1988 Chromatography of the isolated chains of fibrinogen E showed that the alpha(Gly17-Lys78) peptide region itself contains a strong binding site for PMSF-thrombin-Sepharose. Sepharose 160-169 fibrinogen beta chain Homo sapiens 41-51 3378041-4 1988 Chromatography of the isolated chains of fibrinogen E showed that the alpha(Gly17-Lys78) peptide region itself contains a strong binding site for PMSF-thrombin-Sepharose. Sepharose 160-169 coagulation factor II, thrombin Homo sapiens 151-159 3378053-2 1988 Purification of L-PN-1 and PN-1 was achieved by fractionating serum-free conditioned culture medium from human fibroblasts over dextran sulfate-Sepharose followed by immunoaffinity fractionation over a PN-1 monoclonal antibody-Sepharose column. Sepharose 144-153 serpin family E member 2 Homo sapiens 16-31 3378053-2 1988 Purification of L-PN-1 and PN-1 was achieved by fractionating serum-free conditioned culture medium from human fibroblasts over dextran sulfate-Sepharose followed by immunoaffinity fractionation over a PN-1 monoclonal antibody-Sepharose column. Sepharose 227-236 serpin family E member 2 Homo sapiens 16-31 2894373-7 1988 The 130,000-dalton ANP-binding sites bound to a GTP-agarose affinity column, and the specific activity of guanylate cyclase was increased by 90-fold in this fraction. Sepharose 52-59 natriuretic peptide A Bos taurus 19-22 2894373-7 1988 The 130,000-dalton ANP-binding sites bound to a GTP-agarose affinity column, and the specific activity of guanylate cyclase was increased by 90-fold in this fraction. Sepharose 52-59 guanylate cyclase Bos taurus 106-123 3128293-1 1988 Aldose reductase from human placenta was purified to homogeneity by a rapid (2 day) and efficient purification scheme involving Red Sepharose affinity chromatography, chromatofocusing and high performance liquid chromatography on a size-exclusion column. Sepharose 132-141 aldo-keto reductase family 1 member B Homo sapiens 0-16 3348810-0 1988 Tight binding of glucocorticoid-receptor complexes to histone-agarose. Sepharose 62-69 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 17-40 3348810-1 1988 "Activated" glucocorticoid-receptor complexes purified about 3,000-fold from rat liver were found to bind to histone-agarose. Sepharose 117-124 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 12-35 3348810-3 1988 This interaction of "activated" glucocorticoid-receptor complexes of rat liver with histone-agarose suggests a role of histones in the mechanism of action of steroid hormone. Sepharose 92-99 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 32-55 3355583-7 1988 GST isoenzymes were purified on S-hexylglutathione Sepharose 6B and separated by means of FPLC-chromatofocusing, to evaluate effects on the GST isoenzyme pattern. Sepharose 51-60 hematopoietic prostaglandin D synthase Rattus norvegicus 0-3 3286063-0 1988 Application of heparin-conjugated Sepharose for the measurement of cobalamin-saturated and unsaturated transcobalamin II. Sepharose 34-43 transcobalamin 2 Homo sapiens 103-120 3286063-3 1988 Transcobalamin II is adsorbed from human plasma to heparin-conjugated Sepharose under suitable conditions and either cobalamin from adsorbed holo-transcobalamin II is measured by a radioisotope dilution assay or apo-transcobalamin II is determined by measuring the adsorbed unsaturated cobalamin-binding capacity with radioactive cobalamin. Sepharose 70-79 transcobalamin 2 Homo sapiens 0-17 2965579-2 1988 In the first system, insulin receptor was purified partially from Fao rat hepatoma cells by direct solubilization of the cells in Triton X-100 and chromatography on wheat-germ-agglutinin-agarose. Sepharose 187-194 insulin receptor Rattus norvegicus 21-37 3257755-3 1988 The binding site of myosin was studied by using caldesmon-Sepharose 4B affinity chromatography. Sepharose 58-70 myosin heavy chain 14 Homo sapiens 20-26 3257755-3 1988 The binding site of myosin was studied by using caldesmon-Sepharose 4B affinity chromatography. Sepharose 58-70 caldesmon 1 Homo sapiens 48-57 3378062-3 1988 At the final step of P-450Coh purification, variously substituted Sepharoses (hydroxyphenyl-, cholate-, aminooctyl- and t-cytochrome-b5-) were used. Sepharose 66-76 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 21-29 2832099-1 1988 This new bioassay for parathyrin (PTH) in plasma (bio-PTH) combines immunoextraction on affinity columns [goat anti-hPTH (1-44) conjugated to Sepharose 4B] and a receptor assay involving an osteosarcoma cell line. Sepharose 142-154 parathyroid hormone Homo sapiens 22-32 2832099-1 1988 This new bioassay for parathyrin (PTH) in plasma (bio-PTH) combines immunoextraction on affinity columns [goat anti-hPTH (1-44) conjugated to Sepharose 4B] and a receptor assay involving an osteosarcoma cell line. Sepharose 142-154 parathyroid hormone Homo sapiens 34-37 2460305-11 1988 The identification of acidic fibroblast growth factor (FGF)in heparin-sepharose-purified material was accomplished by immunoblot experiments involving antibodies against acidic and basic FGF. Sepharose 70-79 fibroblast growth factor 1 Mus musculus 22-53 2460305-11 1988 The identification of acidic fibroblast growth factor (FGF)in heparin-sepharose-purified material was accomplished by immunoblot experiments involving antibodies against acidic and basic FGF. Sepharose 70-79 fibroblast growth factor 1 Mus musculus 55-58 3181034-6 1988 The chick MIF seemed somewhat heterogeneous by chromatofocusing and a portion of its activity bound to heparin sepharose. Sepharose 111-120 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Gallus gallus 10-13 3277840-4 1988 Purification of adipocyte insulin receptors by wheat germ agglutinin-Sepharose chromatography, followed by insulin-agarose affinity chromatography, resulted in loss of DTT stimulation of insulin binding. Sepharose 69-78 insulin Homo sapiens 26-33 3277840-4 1988 Purification of adipocyte insulin receptors by wheat germ agglutinin-Sepharose chromatography, followed by insulin-agarose affinity chromatography, resulted in loss of DTT stimulation of insulin binding. Sepharose 115-122 insulin Homo sapiens 26-33 3277840-4 1988 Purification of adipocyte insulin receptors by wheat germ agglutinin-Sepharose chromatography, followed by insulin-agarose affinity chromatography, resulted in loss of DTT stimulation of insulin binding. Sepharose 115-122 insulin Homo sapiens 107-114 3277840-4 1988 Purification of adipocyte insulin receptors by wheat germ agglutinin-Sepharose chromatography, followed by insulin-agarose affinity chromatography, resulted in loss of DTT stimulation of insulin binding. Sepharose 115-122 insulin Homo sapiens 107-114 3342932-3 1988 Heparin-induced inhibition of skeletal muscle growth is a consequence of its interaction with a growth factor(s) present in the media used to support myogenesis; heparin-Sepharose column absorbed horse serum can support muscle growth only in the presence of added heparin-binding growth factors like fibroblast growth factor (FGF) or chicken muscle growth factor (CMGF). Sepharose 170-179 fibroblast growth factor 10 Gallus gallus 300-324 3258127-8 1988 In addition, large granulomas were induced by the intratracheal injection of recombinant IL-1-coated Sepharose 4B beads. Sepharose 101-113 interleukin 1 complex Mus musculus 89-93 3342932-3 1988 Heparin-induced inhibition of skeletal muscle growth is a consequence of its interaction with a growth factor(s) present in the media used to support myogenesis; heparin-Sepharose column absorbed horse serum can support muscle growth only in the presence of added heparin-binding growth factors like fibroblast growth factor (FGF) or chicken muscle growth factor (CMGF). Sepharose 170-179 fibroblast growth factor 10 Gallus gallus 326-329 2832163-14 1988 An improved method for the purification of sulfite oxidase from chicken liver, using affinity chromatography on cytochrome c--Sepharose, is described. Sepharose 126-135 sulfite oxidase Gallus gallus 43-58 3350007-1 1988 Four distinct tyrosine protein kinases active on poly(Glu4,Tyr1) and angiotensin II, and operationally termed TPK-I, TPK-IIA, TPK-IIB and TPK-III have been resolved and partially purified from rat spleen particulate fraction by combining DEAE-Sepharose, heparin-Sepharose, phosphocellulose and polylysine-agarose chromatographies. Sepharose 243-252 angiotensinogen Rattus norvegicus 69-83 2832163-14 1988 An improved method for the purification of sulfite oxidase from chicken liver, using affinity chromatography on cytochrome c--Sepharose, is described. Sepharose 126-135 cytochrome c, somatic Gallus gallus 112-124 3350007-1 1988 Four distinct tyrosine protein kinases active on poly(Glu4,Tyr1) and angiotensin II, and operationally termed TPK-I, TPK-IIA, TPK-IIB and TPK-III have been resolved and partially purified from rat spleen particulate fraction by combining DEAE-Sepharose, heparin-Sepharose, phosphocellulose and polylysine-agarose chromatographies. Sepharose 262-271 angiotensinogen Rattus norvegicus 69-83 3350007-1 1988 Four distinct tyrosine protein kinases active on poly(Glu4,Tyr1) and angiotensin II, and operationally termed TPK-I, TPK-IIA, TPK-IIB and TPK-III have been resolved and partially purified from rat spleen particulate fraction by combining DEAE-Sepharose, heparin-Sepharose, phosphocellulose and polylysine-agarose chromatographies. Sepharose 305-312 angiotensinogen Rattus norvegicus 69-83 2830314-5 1988 In contrast, rH TNF-alpha reduced or abolished neutrophil locomotion under agarose in response to a gradient of FMLP. Sepharose 75-82 tumor necrosis factor Homo sapiens 16-25 3391636-1 1988 The second component of human complement (C2) in pseudoglobulin prepared from normal plasma eluted as a single peak at high conductivity (30 mS) and pH 4.5 from the cationic exchangers S-Sepharose or Mono S in the Fast Protein Liquid Chromatography (FPLC) System. Sepharose 187-196 complement C2 Homo sapiens 30-44 2830314-5 1988 In contrast, rH TNF-alpha reduced or abolished neutrophil locomotion under agarose in response to a gradient of FMLP. Sepharose 75-82 formyl peptide receptor 1 Homo sapiens 112-116 2965209-6 1988 Interestingly, the antigenic crossreactivity between gpIIb/IIIa and the phagocyte receptors purified on peptide-Sepharose is largely in the beta chain of the receptors. Sepharose 112-121 integrin subunit alpha 2b Homo sapiens 53-58 3346730-5 1988 AChE from muscle binds to DBA- and VVA-B4-agarose, and is thereby identified as a glycoconjugate bearing the synapse-specific carbohydrate. Sepharose 42-49 acetylcholinesterase Rattus norvegicus 0-4 3282125-3 1988 Of the six anti-PgR antibodies tested, one (alpha PR6) precipitates murine PgR in an assay using protein A-sepharose as an absorbent for the antibody. Sepharose 107-116 progesterone receptor Mus musculus 16-19 3282125-3 1988 Of the six anti-PgR antibodies tested, one (alpha PR6) precipitates murine PgR in an assay using protein A-sepharose as an absorbent for the antibody. Sepharose 107-116 progesterone receptor Mus musculus 75-78 3371547-6 1988 Northern blot analysis of poly(A)+ and total RNA showed that arginine esterase mRNA had an approximate size of 1.0 kb which corresponded to a major androgen-regulated RNA species that could be observed after denaturing agarose gel electrophoresis of prostatic poly(A)+ RNA from intact dogs. Sepharose 219-226 arginine esterase Canis lupus familiaris 61-78 3391999-1 1988 Lysophospholipase released from rat platelets upon activation with thrombin has been purified to near homogeneity by sequential column chromatography on heparin-Sepharose, CM-Sephadex C-50, and TSK gel G2000SW. Sepharose 161-170 asparaginase Rattus norvegicus 0-17 2963818-4 1988 IL-1 receptors extracted from EL-4 6.1 C10 cells can be bound to and specifically eluted from IL-1 alpha coupled to Sepharose. Sepharose 116-125 interleukin 1 alpha Mus musculus 94-104 2829978-7 1988 In particular, protein phosphatase T is endowed with phosphorylase phosphatase activity that is stimulated by protamine, histone H1 and heparin, it is inhibited by spermine, it does not bind to heparin-Sepharose and it readily dephosphorylates the phosphopeptide Arg-Arg-Leu-Ser(P)-Ile-Ser-Thr-Glu-Ser reproducing the phosphorylation site of the alpha-subunit of phosphorylase kinase. Sepharose 202-211 protein phosphatase 5, catalytic subunit Rattus norvegicus 15-36 3363529-5 1988 Affinity adsorption of affected members" plasma to heparin-sepharose beads revealed one population of ATIII in the supernatant corresponding to the abnormal ATIII, devoid of heparin cofactor activity and showing a peak between pH range: 4.6-4.8 in CIEF. Sepharose 59-68 serpin family C member 1 Homo sapiens 102-107 2963863-7 1988 This specificity of binding and the ability to rebind to a second column of iC3- or C3b-thiol-Sepharose are comparable to human CR1. Sepharose 94-103 endogenous retrovirus group K member 3 Homo sapiens 84-87 2828365-7 1988 u-PA-R retained its ability to bind to ATF after cell lysis, and it was purified approximately 2,200-fold from biosynthetically labeled U937 cells by affinity chromatography with proenzyme u-PA coupled to Sepharose. Sepharose 205-214 plasminogen activator, urokinase receptor Homo sapiens 0-6 2828365-7 1988 u-PA-R retained its ability to bind to ATF after cell lysis, and it was purified approximately 2,200-fold from biosynthetically labeled U937 cells by affinity chromatography with proenzyme u-PA coupled to Sepharose. Sepharose 205-214 plasminogen activator, urokinase Homo sapiens 0-4 3337898-6 1988 The IgG covalently cross-linked to Sepharose 4B bound 125I-PAP p37 but not 125I-fibrinogen. Sepharose 35-47 nucleoporin 37 Homo sapiens 63-66 3123493-3 1988 Antibody to CD3 (a component of the T cell receptor) coupled to agarose or PHA (greater than 3 micrograms/ml) induced both IL 2 production and receptor expression on II23 cells. Sepharose 64-71 interleukin 2 Homo sapiens 123-127 3123493-6 1988 A Mr 70,000 protein with a pI of 6.2 was phosphorylated in hybrids stimulated by PMA, anti-CD3 antibody coupled to agarose or PHA, i.e. by the same stimuli which induce IL 2 receptors on these cells. Sepharose 115-122 interleukin 2 Homo sapiens 169-173 3369689-0 1988 Affinity purification of cytosolic epoxide hydrolase using derivatized epoxy-activated Sepharose gels. Sepharose 87-96 epoxide hydrolase 2, cytoplasmic Mus musculus 25-52 3369689-7 1988 MCT-Sepharose and benzylthio-Sepharose provide rapid and convenient one-step procedures for obtaining purified cytosolic epoxide hydrolase from numerous species and tissues. Sepharose 4-13 epoxide hydrolase 2, cytoplasmic Mus musculus 111-138 3123483-8 1988 Moreover, 125I-labeled t-PA immobilized on Sepharose added directly to endothelial cells formed a complex with PAI-1 in a dose-dependent manner. Sepharose 43-52 plasminogen activator, tissue type Homo sapiens 23-27 3123483-8 1988 Moreover, 125I-labeled t-PA immobilized on Sepharose added directly to endothelial cells formed a complex with PAI-1 in a dose-dependent manner. Sepharose 43-52 serpin family E member 1 Homo sapiens 111-116 3123483-9 1988 On the other hand, no detectable complex was formed with PAI-1 when Sepharose-immobilized 125I-labeled t-PA was added to endothelial cells under conditions in which the added t-PA could not contact the cells directly but other proteins could pass freely by the use of a Transwell. Sepharose 68-77 plasminogen activator, tissue type Homo sapiens 103-107 3364725-2 1988 66, p. 686, Academic Press, New York) we described the preparation and use of purified milk folate-binding protein covalently linked to a Sepharose matrix. Sepharose 138-147 glycine N-methyltransferase Rattus norvegicus 92-114 3126793-5 1988 In the two patients, the highest molecular weight multimers (HMWM) of vWf were absent when assessed by sodium dodecyl-sulphate agarose plasma electrophoresis. Sepharose 127-134 von Willebrand factor Homo sapiens 70-73 3337898-9 1988 The IgG that was bound to PAP p37 conjugated to Sepharose 4B inhibited the agglutination of washed platelets induced by TTP plasma containing PAP p37, whereas the IgG that was not bound to PAP p37 did not have a significant inhibitory effect. Sepharose 48-60 nucleoporin 37 Homo sapiens 30-33 3337898-9 1988 The IgG that was bound to PAP p37 conjugated to Sepharose 4B inhibited the agglutination of washed platelets induced by TTP plasma containing PAP p37, whereas the IgG that was not bound to PAP p37 did not have a significant inhibitory effect. Sepharose 48-60 nucleoporin 37 Homo sapiens 146-149 3337898-9 1988 The IgG that was bound to PAP p37 conjugated to Sepharose 4B inhibited the agglutination of washed platelets induced by TTP plasma containing PAP p37, whereas the IgG that was not bound to PAP p37 did not have a significant inhibitory effect. Sepharose 48-60 nucleoporin 37 Homo sapiens 146-149 3267107-4 1988 These IL-1-like antibodies were affinity purified either on an anti-IL-1-enriched Ig-Sepharose 4B column from an early bleed or sequentially on anti-Ig and Protein A immunoadsorbent columns. Sepharose 85-97 interleukin 1 complex Mus musculus 6-10 3356754-2 1988 In this report, we describe the purification of MGSA from acid ethanol extracts of Hs294T tumors grown in nude mice using a series of Bio-Gel P30, reverse phase-high performance liquid chromatography and heparin-sepharose steps. Sepharose 212-221 chemokine (C-X-C motif) ligand 1 Mus musculus 48-52 3372491-5 1988 By taking advantage of this unique property of anhydrochymotrypsin-Sepharose in combination with reversed-phase high-performance liquid chromatography, we have succeeded in separating the C-terminal peptides from chymotryptic digests of reduced and S-carboxymethylated bovine insulin and from tryptic digests from reduced and S-carboxymethylated Streptomyces subtilisin inhibitor. Sepharose 67-76 insulin Bos taurus 276-283 3335854-1 1988 Benzodiazepine-affinity chromatography, on a column of 1012S-Sepharose, resulted in the detection and purification of a binding protein (P36) from the cytosolic fraction of pig cerebral cortex. Sepharose 61-70 annexin A2 Sus scrofa 137-140 3371070-1 1988 Bovine vitreous body and aorta contain extractable leukocyte elastase inhibitors, which were purified by gel filtration and affinity chromatography on agarose-pancreatic elastase. Sepharose 151-158 elastase, neutrophil expressed Homo sapiens 51-69 3135485-4 1988 The mRNA encoding rABP could be detected in the testes of 20- and 46-day-old-rats, but not in the 10-day-old rats by hybridization with 32P-labeled rABP cDNA in a Northern blot of poly(A)+-RNA fractioned by agarose gel electrophoresis. Sepharose 207-214 sex hormone binding globulin Rattus norvegicus 18-22 3277180-5 1988 The 26-base-pair OBF1-protected sequence was sufficient for the recognition and binding of the protein, since the mobility of a DNA fragment containing the synthetic binding site was retarded in agarose gels when incubated with OBF1. Sepharose 195-202 DNA-binding protein ABF1 Saccharomyces cerevisiae S288C 17-21 3277180-5 1988 The 26-base-pair OBF1-protected sequence was sufficient for the recognition and binding of the protein, since the mobility of a DNA fragment containing the synthetic binding site was retarded in agarose gels when incubated with OBF1. Sepharose 195-202 DNA-binding protein ABF1 Saccharomyces cerevisiae S288C 228-232 16665943-1 1988 Two different families of hydroxyproline-rich glycoproteins, HRGP(1) and HRGP(2), have been isolated from melon callus and separated by ion exchange chromatography on CM-sepharose. Sepharose 170-179 histidine rich glycoprotein Homo sapiens 61-65 3335854-10 1988 However, the ability of the gamma-aminobutyrate/benzodiazepine receptor inverse agonists, methyl- and ethyl-beta-carboline-3-carboxylate, to inhibit the binding of P36 to 1012S-Sepharose at relatively low concentrations indicates that P36 exhibits a degree of binding specificity. Sepharose 177-186 annexin A2 Sus scrofa 164-167 3276308-3 1988 A specific binding of SAP to hyaluronic acid, heparan sulfate, and dermatan sulfate was also confirmed by the fact that these glycosaminoglycans blocked the binding of SAP to agarose, a specific ligand of SAP. Sepharose 175-182 amyloid P component, serum Homo sapiens 22-25 3399792-1 1988 Fibronectin has been purified by gelatin-Sepharose affinity chromatography from fresh frozen human plasma. Sepharose 41-50 fibronectin 1 Homo sapiens 0-11 2893611-3 1988 We also describe a subline of these cells which are relatively resistant to the transforming growth factor-beta-induced effects on proliferation in monolayer and in soft agarose, but which retain the ability to specifically bind transforming growth factor-beta. Sepharose 170-177 transforming growth factor beta 1 Homo sapiens 80-111 3276308-3 1988 A specific binding of SAP to hyaluronic acid, heparan sulfate, and dermatan sulfate was also confirmed by the fact that these glycosaminoglycans blocked the binding of SAP to agarose, a specific ligand of SAP. Sepharose 175-182 amyloid P component, serum Homo sapiens 168-171 3276308-3 1988 A specific binding of SAP to hyaluronic acid, heparan sulfate, and dermatan sulfate was also confirmed by the fact that these glycosaminoglycans blocked the binding of SAP to agarose, a specific ligand of SAP. Sepharose 175-182 amyloid P component, serum Homo sapiens 168-171 3338465-7 1988 The two adenylyl cyclases from brain can be separated by calmodulin-Sepharose: only the enzyme of 115 kDa but not that of 150 kDa was retained by the affinity resin and could be stimulated by Ca2+/calmodulin. Sepharose 68-77 calmodulin Bos taurus 57-67 3338465-7 1988 The two adenylyl cyclases from brain can be separated by calmodulin-Sepharose: only the enzyme of 115 kDa but not that of 150 kDa was retained by the affinity resin and could be stimulated by Ca2+/calmodulin. Sepharose 68-77 calmodulin Bos taurus 197-207 3338470-1 1988 Cathepsin B was purified from rabbit skeletal muscle by ammonium sulfate fractionation and successive chromatographies on Sephadex G-75, phosphocellulose, peptide-conjugated Sepharose, DEAE-Toyopearl and Sephadex G-100. Sepharose 174-183 cathepsin B Oryctolagus cuniculus 0-11 3350116-2 1988 The enzyme has been separated from the endoplasmic reticulum using Triton X-114 and further purified using NAD to release glucose dehydrogenase from a NADP-linked sepharose column. Sepharose 163-172 hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Homo sapiens 122-143 3135686-1 1988 Colony-stimulating factor (CSF) was partially purified from urine of patients with aplastic anemia using DEAE-cellulose and concanavalin A-Sepharose. Sepharose 139-148 colony stimulating factor 2 Homo sapiens 0-25 2833568-6 1988 Sepharose-Protein A affinity-purified isotypes were also studied and showed IgG1 greater than IgG2a greater than or equal to IgG2b much greater than IgG3 activity per microgram of isotype, indicating that competition between isotypes present in high concentrations did not significantly alter the results. Sepharose 0-9 LOC105243590 Mus musculus 76-80 2833568-6 1988 Sepharose-Protein A affinity-purified isotypes were also studied and showed IgG1 greater than IgG2a greater than or equal to IgG2b much greater than IgG3 activity per microgram of isotype, indicating that competition between isotypes present in high concentrations did not significantly alter the results. Sepharose 0-9 immunoglobulin heavy variable V1-9 Mus musculus 94-99 2833568-6 1988 Sepharose-Protein A affinity-purified isotypes were also studied and showed IgG1 greater than IgG2a greater than or equal to IgG2b much greater than IgG3 activity per microgram of isotype, indicating that competition between isotypes present in high concentrations did not significantly alter the results. Sepharose 0-9 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 149-153 2976253-3 1988 The D2 dopamine receptor protein was partially purified by Fast Performance Liquid Chromatography on a Mono Q column and on a wheat-germ agglutinin agarose column. Sepharose 148-155 dopamine receptor D2 Homo sapiens 4-24 2969693-1 1988 A fibronectin binding protein (FnBp) was identified in 3H isoleucine labeled P. falciparum schizonts using affinity chromatography on human fibronectin (Fn) coupled to Sepharose 4B. Sepharose 168-177 fibronectin 1 Homo sapiens 2-13 3135686-1 1988 Colony-stimulating factor (CSF) was partially purified from urine of patients with aplastic anemia using DEAE-cellulose and concanavalin A-Sepharose. Sepharose 139-148 colony stimulating factor 2 Homo sapiens 27-30 2969693-1 1988 A fibronectin binding protein (FnBp) was identified in 3H isoleucine labeled P. falciparum schizonts using affinity chromatography on human fibronectin (Fn) coupled to Sepharose 4B. Sepharose 168-177 fibronectin 1 Homo sapiens 31-33 2969693-2 1988 After incubation of Nonidet-P 40 parasite lysate with Fn-Sepharose, elution was performed with SDS-PAGE buffer. Sepharose 57-66 fibronectin 1 Homo sapiens 54-56 3203157-3 1988 Furthermore, brain actomyosin was retained on a column of conjugated calmodulin-Sepharose 4B and eluted by 6 M urea in the presence of Ca2+. Sepharose 80-89 calmodulin Oryctolagus cuniculus 69-79 2478092-0 1988 [Electrophoresis of unconcentrated CSF proteins in agarose gels. Sepharose 51-58 colony stimulating factor 2 Homo sapiens 35-38 3129035-6 1988 The Km value of phenylalanine for the cytoplasmic form of phenylalanine hydroxylase is 0.32.10(-3) M, that for the membrane form is 1.66.10(-3) M. Both enzyme forms can bind to phenyl-Sepharose after their activation by the substrate, and they dissociate from the carrier after phenylalanine removal from the incubation mixture, which points to the intactness of the phenylalanine binding allosteric center in the membrane form of the enzyme. Sepharose 184-193 phenylalanine hydroxylase Rattus norvegicus 58-83 3120820-3 1988 The factor VIII in the concentrate was isolated by tandem chromatography on gelatin-Sepharose and monoclonal anti-factor VIII-Sepharose. Sepharose 84-93 coagulation factor VIII Sus scrofa 4-15 3120820-3 1988 The factor VIII in the concentrate was isolated by tandem chromatography on gelatin-Sepharose and monoclonal anti-factor VIII-Sepharose. Sepharose 126-135 coagulation factor VIII Sus scrofa 4-15 3120820-3 1988 The factor VIII in the concentrate was isolated by tandem chromatography on gelatin-Sepharose and monoclonal anti-factor VIII-Sepharose. Sepharose 126-135 coagulation factor VIII Sus scrofa 114-125 2456179-5 1988 Stickleback Vg can be purified by con A-Sepharose chromatography. Sepharose 40-49 vitellogenin Gasterosteus aculeatus 12-14 3276312-1 1988 This report describes the purification of placental protein 5, PP5, from the human placenta by two affinity chromatography steps, the first with Heparin-Sepharose and the second with Sepharose-linked monoclonal anti-PP5 antibody. Sepharose 153-162 tissue factor pathway inhibitor 2 Homo sapiens 42-61 3276312-1 1988 This report describes the purification of placental protein 5, PP5, from the human placenta by two affinity chromatography steps, the first with Heparin-Sepharose and the second with Sepharose-linked monoclonal anti-PP5 antibody. Sepharose 183-192 tissue factor pathway inhibitor 2 Homo sapiens 42-61 3276312-1 1988 This report describes the purification of placental protein 5, PP5, from the human placenta by two affinity chromatography steps, the first with Heparin-Sepharose and the second with Sepharose-linked monoclonal anti-PP5 antibody. Sepharose 183-192 tissue factor pathway inhibitor 2 Homo sapiens 63-66 3257921-3 1988 A mitogenic anti-Thy-1 antibody (KT16) made the T cell clone unresponsive to specific antigen and to an anti-T cell receptor antibody coupled to Sepharose. Sepharose 145-154 thymus cell antigen 1, theta Mus musculus 17-22 2448241-2 1988 Tonin-alpha 1-macroglobulin complex, which was immunologically immobilized by anti-alpha 1-macroglobulin antibody covalently coupled to agarose gels, could quantitatively hydrolyze angiotensin I and synthetic tridecapeptide renin substrate to form angiotensin II. Sepharose 136-143 pregnancy-zone protein Rattus norvegicus 83-104 3203499-6 1988 IgG subclass distribution of NEF antibody sera was also investigated by means of protein A-Sepharose and using monoclonal antibodies against human IgG subclasses. Sepharose 91-100 S100 calcium binding protein B Homo sapiens 29-32 3280421-8 1988 An example for the latter is the use of bezamidine-Sepharose for the purification of thrombin. Sepharose 51-60 coagulation factor II, thrombin Homo sapiens 85-93 3280421-10 1988 Antithrombin III can be isolated by linkage to its cofactor, heparin, on heparin-Sepharose. Sepharose 81-90 serpin family C member 1 Homo sapiens 0-16 2448241-2 1988 Tonin-alpha 1-macroglobulin complex, which was immunologically immobilized by anti-alpha 1-macroglobulin antibody covalently coupled to agarose gels, could quantitatively hydrolyze angiotensin I and synthetic tridecapeptide renin substrate to form angiotensin II. Sepharose 136-143 kallikrein 1-related peptidase C2 Rattus norvegicus 0-5 2448241-2 1988 Tonin-alpha 1-macroglobulin complex, which was immunologically immobilized by anti-alpha 1-macroglobulin antibody covalently coupled to agarose gels, could quantitatively hydrolyze angiotensin I and synthetic tridecapeptide renin substrate to form angiotensin II. Sepharose 136-143 renin Rattus norvegicus 224-229 2448241-2 1988 Tonin-alpha 1-macroglobulin complex, which was immunologically immobilized by anti-alpha 1-macroglobulin antibody covalently coupled to agarose gels, could quantitatively hydrolyze angiotensin I and synthetic tridecapeptide renin substrate to form angiotensin II. Sepharose 136-143 pregnancy-zone protein Rattus norvegicus 6-27 2448241-2 1988 Tonin-alpha 1-macroglobulin complex, which was immunologically immobilized by anti-alpha 1-macroglobulin antibody covalently coupled to agarose gels, could quantitatively hydrolyze angiotensin I and synthetic tridecapeptide renin substrate to form angiotensin II. Sepharose 136-143 angiotensinogen Rattus norvegicus 248-262 3192306-3 1988 We present evidence suggestive of varying affinities of SAP for agarose, to which SAP is known to adsorb in the presence of calcium, by fused rocket immunoelectrophoresis. Sepharose 64-71 amyloid P component, serum Homo sapiens 56-59 3181599-2 1988 Polyamine oxidase was purified from the soluble fraction of porcine liver by more than 70,000-fold to electrophoretic homogeneity using N8-acetylspermidine-Sepharose 4B affinity chromatography. Sepharose 156-165 polyamine oxidase Homo sapiens 0-17 3360754-5 1988 The concentration-incubation treatment also increased the proportion of trehalase that interacts with Con A-Sepharose. Sepharose 108-117 trehalase Oryctolagus cuniculus 72-81 3360754-6 1988 These results indicate that kidney trehalase that interacts with Con A-Sepharose. Sepharose 71-80 trehalase Oryctolagus cuniculus 35-44 3192306-3 1988 We present evidence suggestive of varying affinities of SAP for agarose, to which SAP is known to adsorb in the presence of calcium, by fused rocket immunoelectrophoresis. Sepharose 64-71 amyloid P component, serum Homo sapiens 82-85 3172279-1 1988 The major cytosolic and membrane-associated enkephalin-degrading aminopeptidases were purified in parallel by column chromatography successively on DEAE-cellulose, AH-Sepharose, hydroxylapatite, Sephadex G-200, Affigel Blue, AH-Sepharose, and hydroxylapatite. Sepharose 167-176 proenkephalin Rattus norvegicus 44-54 3347046-1 1988 The nontransformed glucocorticoid receptor (GR) from rat liver was found to bind to protamine-Sepharose and could be recovered by a salt gradient without a change in molecular configuration. Sepharose 94-103 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 19-42 3347046-1 1988 The nontransformed glucocorticoid receptor (GR) from rat liver was found to bind to protamine-Sepharose and could be recovered by a salt gradient without a change in molecular configuration. Sepharose 94-103 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 44-46 3347046-2 1988 The nontransformed GR also bound to arginine-Sepharose, but the transformed GR did not bind to either resin. Sepharose 45-54 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 19-21 3347046-4 1988 The bindings of GR to protamine- and arginine-Sepharose were saturable. Sepharose 46-55 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 16-18 3347046-5 1988 The apparent dissociation constants of GR on protamine-Sepharose varied from 0.34 nM (-molybdate) to 0.68 nM (+ 10 mM molybdate) and those on arginine-Sepharose were 1.99 nM (-molybdate) and 0.65 nM (+ 10mM molybdate), respectively. Sepharose 55-64 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 39-41 3347046-5 1988 The apparent dissociation constants of GR on protamine-Sepharose varied from 0.34 nM (-molybdate) to 0.68 nM (+ 10 mM molybdate) and those on arginine-Sepharose were 1.99 nM (-molybdate) and 0.65 nM (+ 10mM molybdate), respectively. Sepharose 151-160 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 39-41 3347046-7 1988 Higher salt concentrations (0.5 M NaCl) were required to elute GR from protamine-Sepharose than from arginine-Sepharose (approx 0.03 M NaCl). Sepharose 81-90 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 63-65 2463441-6 1988 Furthermore, GAT-TsF1 can be specifically eluted from GAT-Sepharose with soluble GAT. Sepharose 58-67 glycine-N-acyltransferase Homo sapiens 13-16 2463441-6 1988 Furthermore, GAT-TsF1 can be specifically eluted from GAT-Sepharose with soluble GAT. Sepharose 58-67 serine/threonine kinase 16 Homo sapiens 17-21 3062274-1 1988 A one-step purification of interleukin-4 is described using an 11B11 monoclonal antibody-Sepharose 4B chromatography column. Sepharose 89-98 interleukin 4 Mus musculus 27-40 2463441-6 1988 Furthermore, GAT-TsF1 can be specifically eluted from GAT-Sepharose with soluble GAT. Sepharose 58-67 glycine-N-acyltransferase Homo sapiens 54-57 2463441-6 1988 Furthermore, GAT-TsF1 can be specifically eluted from GAT-Sepharose with soluble GAT. Sepharose 58-67 glycine-N-acyltransferase Homo sapiens 54-57 3122020-3 1988 Simian virus 40 small T-antigen splice and polyadenylation signals were included to produce a mature transcript coding for t-PA, whose activity can be detected in single cells by a fibrin-agarose plaque assay. Sepharose 188-195 plasminogen activator, tissue Mus musculus 123-127 3275424-9 1988 Removal of FN from EE with gelatin-Sepharose does not remove the ability of EE to stimulate migration. Sepharose 35-44 fibronectin 1 Rattus norvegicus 11-13 2897688-1 1988 Two types of pancreatic gamma-glutamyltranspeptidase (GGTP) (EC 2.3.2.2), sialic acid poor and sialic acid rich, were purified by the following: anion-exchange chromatography, wheat germ agglutinin (WGA)-Sepharose chromatography, gel filtration chromatography, phenyl-Superose chromatography, and hydroxylapatite chromatography. Sepharose 204-213 inactive glutathione hydrolase 2 Homo sapiens 54-58 3374483-12 1988 Tyrosyl-tRNA synthetase from beef liver has non-specific affinity to rRNA-sepharose. Sepharose 74-83 tyrosyl-tRNA synthetase 1 Bos taurus 0-23 20501275-4 1988 Interestingly, phosphorylated IRBP was bound tightly to concanvalin A (Con A)-Sepharose and was not eluted by 50 mM ?-methyl-d-mannoside indicating a marked alteration in binding characteristics upon phosphorylation. Sepharose 78-87 retinol binding protein 3 Bos taurus 30-34 3124306-5 1988 Aggregation of the vWf molecule, detected by altered mobility in crossed immunoelectrophoresis and multimeric analysis in SDS agarose gels, occurred in heated intermediate-purity concentrates but not in fibrinogen-poor concentrates. Sepharose 126-133 von Willebrand factor Homo sapiens 19-22 3121600-4 1987 In contrast, when analyzed by agarose/sodium dodecyl sulfate-polyacrylamide gel electrophoresis, vWF consisted of a population of greater than 10 multimers derived from a 270-kDa monomer. Sepharose 30-37 von Willebrand factor Homo sapiens 97-100 3319615-3 1987 Creatine kinase and hexokinase were covalently attached to Sepharose beads. Sepharose 59-68 hexokinase 1 Homo sapiens 20-30 3320045-7 1987 Papilin forms oligomers linked by disulfide bridges, as shown by sodium dodecyl sulfate-agarose gel electrophoresis and electron microscopy. Sepharose 88-95 Papilin Drosophila melanogaster 0-7 2964864-2 1987 IgG2a-binding protein isolated from the detergent lysate of P388D1 cells by affinity chromatography on IgG-Sepharose was found to contain four distinct proteins of Mr 50,000, 43,000, 37,000, and 17,000, which could be autophosphorylated upon incubation with [gamma-32P]ATP. Sepharose 107-116 immunoglobulin heavy variable V1-9 Mus musculus 0-5 3325043-5 1987 The electrophoretic mobility in agarose of the bacterially expressed isoenzyme suggested that its pI is identical with that of the cationic isoenzyme from human liver previously termed GST2 type 1. Sepharose 32-39 glutathione S-transferase alpha 1 Homo sapiens 185-189 3127917-8 1987 When FVIII-LC was coupled to Sepharose at a concentration of 4800 units of FVIII-LC:Ag per ml Sepharose, 0.2 ml of the immunosorbent was able to bind 900 Bethesda units from 100 ml hemophilia A inhibitor plasma. Sepharose 29-38 coagulation factor VIII Homo sapiens 5-10 3127917-8 1987 When FVIII-LC was coupled to Sepharose at a concentration of 4800 units of FVIII-LC:Ag per ml Sepharose, 0.2 ml of the immunosorbent was able to bind 900 Bethesda units from 100 ml hemophilia A inhibitor plasma. Sepharose 29-38 coagulation factor VIII Homo sapiens 75-80 3127917-8 1987 When FVIII-LC was coupled to Sepharose at a concentration of 4800 units of FVIII-LC:Ag per ml Sepharose, 0.2 ml of the immunosorbent was able to bind 900 Bethesda units from 100 ml hemophilia A inhibitor plasma. Sepharose 94-103 coagulation factor VIII Homo sapiens 5-10 3127917-8 1987 When FVIII-LC was coupled to Sepharose at a concentration of 4800 units of FVIII-LC:Ag per ml Sepharose, 0.2 ml of the immunosorbent was able to bind 900 Bethesda units from 100 ml hemophilia A inhibitor plasma. Sepharose 94-103 coagulation factor VIII Homo sapiens 75-80 3127917-9 1987 This opens the possibility to remove FVIII inhibitor antibodies from circulation by extracorporeal immunotherapy with FVIII-LC coupled to Sepharose. Sepharose 138-147 coagulation factor VIII Homo sapiens 37-42 2448895-0 1987 Chromatography of vWf on dextran sulphate sepharose. Sepharose 42-51 von Willebrand factor Homo sapiens 18-21 2445475-5 1987 gp90 was partially purified from a detergent-solubilized membrane preparation by chromatography on DEAE-Sepharose, hydroxylapatite, chelating Sepharose, and lectin-agarose columns. Sepharose 104-113 galectin 3 binding protein Homo sapiens 0-4 2445475-5 1987 gp90 was partially purified from a detergent-solubilized membrane preparation by chromatography on DEAE-Sepharose, hydroxylapatite, chelating Sepharose, and lectin-agarose columns. Sepharose 164-171 galectin 3 binding protein Homo sapiens 0-4 2826582-9 1987 The composition of the complexes was examined by absorption of serum with F(ab")2 anti-C1s- or anti-C1r-coated Sepharose beads. Sepharose 111-120 complement C1r Homo sapiens 100-103 3430617-4 1987 Cross-linking of non-denatured plectin in solution with dimethyl suberimidate and electrophoretic analyses on sodium dodecyl sulfate/agarose gels revealed that the predominant soluble plectin species was a molecule of 1200 X 10(3) Mr consisting of four 300 X 10(3) Mr polypeptide chains. Sepharose 133-140 plectin Rattus norvegicus 184-191 3680266-14 1987 Selected affinity antibodies specific for apo-B37 were used to prepare an anti-apo-B37-Sepharose 4B column. Sepharose 87-96 atrophin 1 Homo sapiens 83-86 2448895-1 1987 A relatively simple and reproducible chromatographic separation using Dextran Sulphate (DS) Agarose is described for the purification of vWf:Ag from cryoprecipitate or plasma source material. Sepharose 92-99 von Willebrand factor Homo sapiens 137-140 2829832-8 1987 Affinity chromatography on Sepharose-alprenolol and sequential elution with 1 microM-CGP-20712A followed by 100 microM(-)-alprenolol permitted beta 1-adrenergic receptors to be resolved from the mixture of beta 1-/beta 2-adrenergic receptors. Sepharose 27-36 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 143-149 2448895-3 1987 The major contaminant fibronectin can be removed prior to the chromatography step by Gelatin-Sepharose adsorption. Sepharose 93-102 fibronectin 1 Homo sapiens 22-33 2448895-6 1987 DS sepharose chromatography offers an excellent method for the purification of 125I-vWf since all the viable label is resolved from excess free radiolabel and denatured protein. Sepharose 3-12 von Willebrand factor Homo sapiens 84-87 3319692-1 1987 A hepatocyte growth factor (HGF) that stimulates DNA synthesis of adult rat hepatocytes in primary culture was purified as a homogeneous material from platelets of 1000 rats by a four-step procedure: stimulation of its release from platelets by thrombin, cation-exchanger fast protein liquid chromatography (FPLC) on a Mono S column, heparin-Sepharose CL-6B chromatography, and reverse-phase HPLC on a C4 column. Sepharose 342-351 hepatocyte growth factor Rattus norvegicus 2-26 3119264-6 1987 Adsorption of SLE ACS with Sepharose-bound antiserum against IL-1 prevented ACS-induced increases in IgG synthesis. Sepharose 27-36 interleukin 1 alpha Homo sapiens 61-65 3124988-7 1987 Affinity chromatography on monoclonal anti-Apo A-I Sepharose column separates two lipoprotein particles containing Apo S, one retained with Apo A-I (42.5%) and the other eluting without Apo A-I (57.5%). Sepharose 51-60 apolipoprotein A1 Homo sapiens 43-50 2823904-2 1987 Electrophoretically, the Apo-B-containing HDLHuH-7 migrated more slowly and broadly than beta-lipoprotein on agarose gel. Sepharose 109-116 apolipoprotein B Homo sapiens 25-30 3343969-4 1988 Affinity chromatography employing jacalin-Sepharose provided a means to separate the subclasses of IgA in human whey. Sepharose 42-51 immunoglobulin heavy variable 4-38-2-like Homo sapiens 99-102 3343973-1 1988 An IgA1-specific lectin, Jacalin, was isolated from dried seeds of the jackfruit, Artocarpus integrifolia, by affinity binding to IgA1-Sepharose and elution with D-galactose. Sepharose 135-144 immunoglobulin heavy constant alpha 1 Homo sapiens 3-7 3436060-2 1987 Prolidase I activity, which was eluted in chromatographic fractions of low ionic strength of about 160 mmol/l NaCl, increased after preincubation and this activation was maintained throughout the subsequent steps of chromatofocusing, chromatography on Sephacryl S-200 in tandem with Blue-Sepharose, and Phenyl-Sepharose chromatography. Sepharose 288-297 peptidase D Homo sapiens 0-9 3436060-2 1987 Prolidase I activity, which was eluted in chromatographic fractions of low ionic strength of about 160 mmol/l NaCl, increased after preincubation and this activation was maintained throughout the subsequent steps of chromatofocusing, chromatography on Sephacryl S-200 in tandem with Blue-Sepharose, and Phenyl-Sepharose chromatography. Sepharose 310-319 peptidase D Homo sapiens 0-9 3680518-6 1987 Chromatography on gelatin-Sepharose gave a single peak of activity that was predominantly composed of the 90,000-mol-wt proteinase. Sepharose 26-35 endogenous retrovirus group K member 25 Homo sapiens 120-130 3680518-7 1987 The proteolytic activity in the gelatin-Sepharose-purified material was inhibited by EDTA and 1,10-phenanthroline, but not by N-ethylmaleimide or phenylmethanesulfonyl fluoride, indicating that the proteinase was a metalloproteinase. Sepharose 40-49 endogenous retrovirus group K member 25 Homo sapiens 198-208 3448104-5 1987 The well-proven antimicrobial agent, lactoferrin, was isolated from sweet whey by heparin-attached Sepharose. Sepharose 99-108 lactotransferrin Bos taurus 37-48 3319692-1 1987 A hepatocyte growth factor (HGF) that stimulates DNA synthesis of adult rat hepatocytes in primary culture was purified as a homogeneous material from platelets of 1000 rats by a four-step procedure: stimulation of its release from platelets by thrombin, cation-exchanger fast protein liquid chromatography (FPLC) on a Mono S column, heparin-Sepharose CL-6B chromatography, and reverse-phase HPLC on a C4 column. Sepharose 342-351 hepatocyte growth factor Rattus norvegicus 28-31 3324826-1 1987 Agarose gel isoelectrofocusing is used to separate the isozymes of human prostatic acid phosphatase with retention of enzyme activity. Sepharose 0-7 acid phosphatase 3 Homo sapiens 73-99 3124809-1 1987 Crayfish plasma was found to contain a proteinase inhibitor, which was purified to apparent homogeneity by acid precipitation, affinity chromatography on concanavalin A-Sepharose and hydrophobic-interaction chromatography. Sepharose 169-178 endogenous retrovirus group K member 25 Homo sapiens 39-49 3676301-4 1987 95 (1979) 254-259), after which it was further purified using a column of cytochrome c covalently attached to Sepharose 4B. Sepharose 110-122 cytochrome c, somatic Homo sapiens 74-86 3442627-4 1987 The larger of the two, representing 60-63% of plasma transferrin, was bound by concanavalin A - Sepharose, while the smaller one (30-32% of plasma transferrin) resolved into an unbound (25-27% of plasma transferrin) and a retarded (4-5% of plasma transferrin) fraction. Sepharose 96-105 transferrin Rattus norvegicus 53-64 2824663-4 1987 Thymidine kinase from the parental virus bound to DNA-Sepharose, but the truncated enzyme did not. Sepharose 54-63 involved in nucleotide metabolism Human alphaherpesvirus 1 0-16 2445493-9 1987 Moreover, this clone is activated by F23.1, linked to Sepharose 4B beads, which was believed previously to activate only Lyt 2+, L3T4 T cells. Sepharose 54-66 CD8 antigen, alpha chain Mus musculus 121-126 2445493-9 1987 Moreover, this clone is activated by F23.1, linked to Sepharose 4B beads, which was believed previously to activate only Lyt 2+, L3T4 T cells. Sepharose 54-66 CD4 antigen Mus musculus 129-133 3677374-3 1987 The bound TNF is precipitated with Sepharose-bound anti-rabbit IgG, then centrifuged, and the radioactivity of the pellets is counted. Sepharose 35-44 tumor necrosis factor Homo sapiens 10-13 3436963-1 1987 A calmodulin binding portion was separated from chicken gizzard caldesmon by chymotryptic digestion and it was purified through two column chromatography steps on calmodulin-Sepharose and Ultrogel AcA 44. Sepharose 174-183 calmodulin 2 Gallus gallus 2-12 3655326-6 1987 In addition, the morphological appearance of the precipitated reaction product after its changes during the histochemical procedure was monitored using electric eel AChE immobilized on Sepharose 4B. Sepharose 185-197 acetylcholinesterase Rattus norvegicus 165-169 2889750-4 1987 This method involves the selective retention of SS-28 on a conjugate of agarose with immunoglobulins that recognize an epitope in the NH2-terminal region of SS-28. Sepharose 72-79 somatostatin Homo sapiens 48-53 2966503-1 1987 Repeated immunizations of rabbits with chemically synthesized peptides from selected regions of HLA-DP histocompatibility antigens resulted in the production of specific antibodies that were then isolated from the immune sera by chromatography on Sepharose-peptide immunoadsorbents. Sepharose 247-256 major histocompatibility complex, class II, DP beta 1 Homo sapiens 96-102 2822964-5 1987 It is not known whether the kinase involved is of cellular or viral origin, but after purification of NS2 by affinity chromatography on poly(U)-Sepharose it could still by phosphorylated in vitro without the addition of exogenous protein kinase. Sepharose 144-153 NS2 Homo sapiens 102-105 3433251-3 1987 The interaction of both fractions with purified alpha-thrombin was studied by the formation of complexes as well as by affinity chromatography on thrombin-Sepharose. Sepharose 155-164 coagulation factor II, thrombin Homo sapiens 146-154 3667601-5 1987 The fraction of biologically active ATIII has been purified from total ATIII by affinity fractionation on heparin-Sepharose. Sepharose 114-123 serpin family C member 1 Homo sapiens 36-41 3312195-1 1987 A Mr = 110,000 glycoprotein, GP 110, was partially purified using wheat germ agglutinin-Sepharose affinity chromatography from a bile canalicular-enriched membrane fraction denoted N2u of rat liver. Sepharose 88-97 ADRM1 26S proteasome ubiquitin receptor Rattus norvegicus 29-35 3153469-1 1987 The 9S molybdate-stabilized form of the glucocorticoid receptor of mouse L cell lysates was immunoadsorbed to protein-A-Sepharose with antiserum directed against the 89-kilodalton chicken heat shock protein (anti-hsp89). Sepharose 120-129 nuclear receptor subfamily 3, group C, member 1 Mus musculus 40-63 3675582-2 1987 The microsomal fraction containing procathepsin D which passed through a pepstatin-Sepharose resin showed no appreciable activity of cathepsin D. Sepharose 83-92 cathepsin D Rattus norvegicus 38-49 3499103-2 1987 Alpha-1-PI was isolated from rat plasma by affinity chromatography on Sepharose-bound anhydrochymotrypsin, DEAE-cellulose anion-exchange, and Sephadex G-150 gel filtration. Sepharose 70-79 serpin family A member 1 Rattus norvegicus 0-10 3427134-2 1987 The optimal conditions for this binding were elaborated, and the quantitative parameters of Lys-plasminogen binding to fibrinogen-Sepharose were determined. Sepharose 130-139 fibrinogen beta chain Homo sapiens 119-129 3501295-2 1987 Human protein C-inhibitor (PCI) was isolated from human citrated plasma by combining rivanol precipitation, ammonium sulfate precipitation, ion-exchange chromatography on DEAE-Sephacel and affinity chromatography on dextran sulfate Sepharose. Sepharose 232-241 serpin family A member 5 Homo sapiens 6-25 3501295-2 1987 Human protein C-inhibitor (PCI) was isolated from human citrated plasma by combining rivanol precipitation, ammonium sulfate precipitation, ion-exchange chromatography on DEAE-Sephacel and affinity chromatography on dextran sulfate Sepharose. Sepharose 232-241 serpin family A member 5 Homo sapiens 27-30 2960536-5 1987 We describe here that the monoclonal antibody (mAb) 72-5D3 belonging to CD45 (T200), which was not mitogenic by itself, could replace monocytes when MHDC were activated by Sepharose-bound CD3. Sepharose 172-181 protein tyrosine phosphatase receptor type C Homo sapiens 72-76 2959477-5 1987 As determined by competitive radioimmunoassay and protein-A--Sepharose immunoprecipitation, mAb H-20 crossreacts with intact m7G cap structures. Sepharose 61-70 histocompatibility 20 Mus musculus 96-100 2960536-11 1987 On the other hand mAb 72-5D3 induced IL2 production in MHDC activated by Sepharose-bound CD3 and increased the IL2 production in Sepharose-bound CD3-activated peripheral blood mononuclear cells. Sepharose 73-82 interleukin 2 Homo sapiens 37-40 2960536-11 1987 On the other hand mAb 72-5D3 induced IL2 production in MHDC activated by Sepharose-bound CD3 and increased the IL2 production in Sepharose-bound CD3-activated peripheral blood mononuclear cells. Sepharose 129-138 interleukin 2 Homo sapiens 111-114 2822727-11 1987 A single membrane protein with the biochemical characteristics of the class I antigen was isolated on fibronectin-Sepharose and could be immunoprecipitated with the class I monoclonal antibody. Sepharose 114-123 fibronectin 1 Homo sapiens 102-113 3149232-2 1987 TBPA was isolated by retinol-binding protein (RBP) affinity chromatography and further purified by preparative agarose gel electrophoresis or FPLC ion exchange chromatography. Sepharose 111-118 transthyretin Homo sapiens 0-4 3149232-3 1987 TBG was purified by thyroxine (T4)-Sepharose chromatography followed by gel filtration on Sephacryl S-300 and preparative electrofocusing in a granulated dextran gel. Sepharose 35-44 serpin family A member 7 Canis lupus familiaris 0-3 2830246-1 1987 The (H+,K+)ATPase-enriched microsomal fraction prepared from hog gastric mucosa by sucrose density gradient centrifugation was effectively solubilized with Emulgen, with apparent preservation of the enzyme activity, and then the ATPase was highly purified by polyethylene glycol fractionation, and Blue Sepharose CL-6B and amino-hexyl Sepharose chromatographies. Sepharose 303-312 dynein axonemal heavy chain 8 Homo sapiens 11-17 3436952-2 1987 The activity of Lubrol-PX-solubilized acetylcholinesterase was broadly distributed in the fractions having Ve/Vo = 1.0-2.0 in gel filtration on a Sepharose 6B column. Sepharose 146-155 acetylcholinesterase Bos taurus 38-58 2444653-1 1987 Two kinds of keratin-hydrolyzing enzymes (KHEs) from cow snout epithelium were highly purified by affinity chromatography using soybean trypsin inhibitor-bound Sepharose. Sepharose 160-169 kunitz trypsin protease inhibitor Glycine max 136-153 3316976-1 1987 The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Sepharose 489-498 transcription regulator GAL80 Saccharomyces cerevisiae S288C 4-9 3316976-1 1987 The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Sepharose 489-498 galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C 108-112 3316976-1 1987 The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Sepharose 489-498 galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C 108-112 3316976-1 1987 The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Sepharose 489-498 galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C 108-112 3316976-1 1987 The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Sepharose 489-498 galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C 108-112 3316976-1 1987 The GAL80 protein of Saccharomyces cerevisiae, synthesized in vitro, bound tightly to GAL4 protein and to a GAL4 protein-upstream activation sequence DNA complex, as shown by (i) coimmunoprecipitation of GAL4 and GAL80 proteins with anti-GAL4 antiserum, (ii) an electrophoretic mobility shift of a GAL4 protein-upstream activation sequence DNA complex upon the addition of GAL80 protein, and (iii) GAL4-dependent binding of GAL80 protein to upstream activation sequence DNA immobilized on Sepharose beads. Sepharose 489-498 galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C 108-112 3124875-3 1987 Isolation of TPA was done by using a lysine-sepharose affinity chromatoculumn, then, TPA was measured by a fibrin plate method. Sepharose 44-53 chromosome 20 open reading frame 181 Homo sapiens 13-16 3683737-9 1987 Affinity chromatography with a substrate analogue to arginine ester bound to Sepharose beads was also used to isolate de novo synthesized gamma-NGF. Sepharose 77-86 kallikrein 1-related peptidase b3 Mus musculus 138-147 3685455-4 1987 Calcitonin (CT) was immunoextracted from milk by means of CT antibodies coupled to Sepharose 4B, and the extracts were gel-chromatographed on Sephadex G-75 after treatment with 6 M guanidine HCl. Sepharose 83-95 calcitonin related polypeptide alpha Homo sapiens 0-10 2442256-4 1987 Anti-Ig/Sepharose induced a prominent increase in the synthesis and abundance of a 40 kDa/pI 5 nuclear protein (p40/pI-5). Sepharose 8-17 interleukin 9 Mus musculus 112-120 2442256-5 1987 Inhibition of anti-Ig/Sepharose-induced mitogenesis by pretreatment of the cells with phorbol-12-myristate-13-acetate was associated with a specific inhibition (63%) of p40/pI-5. Sepharose 22-31 interleukin 9 Mus musculus 169-177 3620504-3 1987 In the present study, native 20-kDa GH was isolated from a human GH dimer concentrate and purified by a procedure that included column electrophoresis in agarose suspension as a final separation step. Sepharose 154-161 growth hormone 1 Homo sapiens 36-38 2821539-9 1987 The rEC-SOD produced is type C, since its affinity for heparin-Sepharose was identical to that of nEC-SOD type C. Both enzymes bound to concanavalin A, lentil lectin, and wheat germ lectin and are thus glycoproteins. Sepharose 63-72 superoxide dismutase 3 Rattus norvegicus 4-11 3653103-8 1987 The p68 kinase binds to heparin-Sepharose. Sepharose 32-41 eukaryotic translation initiation factor 2 alpha kinase 2 Homo sapiens 4-14 2887565-2 1987 The atrial natriuretic factor (ANF) receptor of bovine adrenal cortex was solubilized with Triton X-100 and purified by sequential chromatography on ANF-(99-126)-agarose, GTP-agarose, and wheat germ agglutinin-Sepharose. Sepharose 210-219 natriuretic peptide A Bos taurus 4-29 3624260-2 1987 Cytochrome P-450 could be quantitated by its CO difference spectrum only after partial purification from the microsomal membrane, and this was achieved by chromatography on p-chloroamphetamine-coupled Sepharose. Sepharose 201-210 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 0-16 3676295-4 1987 We now show that a major mitogenic fraction, isolated from heparin-Sepharose-purified material by Mono-S cation-exchange chromatography and reverse-phase high-performance liquid chromatography, is related to acidic fibroblast growth factor (aFGF). Sepharose 67-76 fibroblast growth factor 1 Bos taurus 208-239 3676295-4 1987 We now show that a major mitogenic fraction, isolated from heparin-Sepharose-purified material by Mono-S cation-exchange chromatography and reverse-phase high-performance liquid chromatography, is related to acidic fibroblast growth factor (aFGF). Sepharose 67-76 fibroblast growth factor 1 Bos taurus 241-245 3114255-6 1987 Furthermore, beta gamma bound to calmodulin-Sepharose in the presence of Ca2+, but not in its absence. Sepharose 44-53 calmodulin 1 Rattus norvegicus 33-43 3040106-6 1987 Three isoenzymes of myeloperoxidase, prepared by gradient elution from a CM-Sepharose column, underwent quantitative analysis. Sepharose 76-85 myeloperoxidase Homo sapiens 20-35 2447894-1 1987 A specific antibody population against human fibrinogen was isolated from a rabbit antiserum by affinity chromatography on fibrinogen-bound Sepharose gel. Sepharose 140-149 fibrinogen beta chain Homo sapiens 45-55 3620487-8 1987 About 80% of trichloroacetic acid-precipitable products could be immunoadsorbed on anti-apolipoprotein A-I antibody immobilized on CNBr-activated Sepharose, suggesting the presence of fragments containing apolipoprotein A-I. Sepharose 146-155 apolipoprotein A1 Rattus norvegicus 88-106 3425896-1 1987 An efficient procedure for affinity purification of human tissue factor apoprotein that requires binding of only microgram quantities of human factor VII to anti-factor VII agarose is described. Sepharose 173-180 coagulation factor III, tissue factor Homo sapiens 58-71 3425896-3 1987 The resultant factor VII/tissue factor/calcium complex was bound to anti-factor VII-agarose, and the bound tissue factor was then eluted with EDTA. Sepharose 84-91 coagulation factor III, tissue factor Homo sapiens 25-38 3427221-5 1987 By affinity chromatography on blue Sepharose a binding of EF2 and of ADP-ribosyl-EF2 with the dye is also demonstrated. Sepharose 35-44 eukaryotic translation elongation factor 2 Homo sapiens 58-61 3427221-5 1987 By affinity chromatography on blue Sepharose a binding of EF2 and of ADP-ribosyl-EF2 with the dye is also demonstrated. Sepharose 35-44 eukaryotic translation elongation factor 2 Homo sapiens 81-84 3427221-6 1987 GDP, GTP and GDP(CH2)P are able to displace EF2 from blue Sepharose. Sepharose 58-67 eukaryotic translation elongation factor 2 Homo sapiens 44-47 3440085-2 1987 In this report we demonstrate that gp46 binds specifically to gelatin-Sepharose and in this respect is similar to a glycoprotein of the molecular mass 47,000, which has earlier been described as a cell surface localized protein in mouse parietal endoderm cells and in chick embryo fibroblasts. Sepharose 70-79 serine (or cysteine) peptidase inhibitor, clade H, member 1 Mus musculus 35-39 3305372-1 1987 Actin was purified from Candida albicans cells by affinity chromatography by DNase-Sepharose and was recognized by immunoblotting with monoclonal antibody directed against chick muscle actin. Sepharose 83-92 actin, beta Gallus gallus 0-5 3123472-2 1987 As the last step of the purification, affinity chromatography was performed either on N-acetylglucosamine-Sepharose or on alpha-lactalbumin-Sepharose: in both cases, two protein bands with molecular weights of around 68,000 and 59,000 were detected by SDS-polyacrylamide gel electrophoresis of the purified preparations. Sepharose 140-149 lactalbumin alpha Homo sapiens 122-139 3305576-5 1987 Wheat germ agglutinin-conjugated Sepharose 4B specifically bound the solubilized cytochrome b and afforded a threefold purification. Sepharose 33-45 cob Triticum aestivum 81-93 3040854-5 1987 Affinity purified goat anti-human IgA bound to Sepharose also augmented DNA synthesis. Sepharose 47-56 CD79a molecule Homo sapiens 34-37 3040854-9 1987 These data indicate that Sepharose-bound antigen was sufficient to induce proliferation and augment IgA secretion by this membrane IgA anti-PC-bearing hybridoma. Sepharose 25-34 CD79a molecule Homo sapiens 100-103 3040854-9 1987 These data indicate that Sepharose-bound antigen was sufficient to induce proliferation and augment IgA secretion by this membrane IgA anti-PC-bearing hybridoma. Sepharose 25-34 CD79a molecule Homo sapiens 131-134 3686888-2 1987 Cathepsin D, isolated from the follicles by means of ammonium sulfate fractionation, chromatography on Hb-Sepharose and Sephadex G-200, was similar to the enzyme from other tissues in molecular mass, Km for hemoglobin, pH optimum of activity, thermolability. Sepharose 106-115 cathepsin D Rattus norvegicus 0-11 2443135-2 1987 Insulin-like growth factor (IGF) binding protein has been purified from adult rat serum by affinity chromatography on agarose-IGF-II and high performance reverse-phase chromatography. Sepharose 118-125 insulin like growth factor 2 Homo sapiens 126-132 2442263-2 1987 The binding of MoAbs to AFP was studied in different experimental systems: crossed affinity immunoelectrophoresis, rocket affinity immunoelectrophoresis and zone affinity electrophoresis in agarose. Sepharose 190-197 alpha fetoprotein Homo sapiens 24-27 3040719-12 1987 Both H1 and H2 were purified to homogeneity when Triton X-100-solubilized membrane proteins from [35S]cysteine-labeled cells were subjected to affinity chromatography on galactose-agarose. Sepharose 180-187 H1.5 linker histone, cluster member Homo sapiens 5-14 3040719-14 1987 Both quantitative immunoprecipitation of each polypeptide from HepG2 cells and the recovery of purified H1 and H2 from galactose-agarose affinity chromatography indicate that there is 5-6 times more H1 relative to H2. Sepharose 129-136 H1.5 linker histone, cluster member Homo sapiens 104-113 3620475-0 1987 Fibrinogen-sepharose interaction with prothrombin, prethrombin 1, prethrombin 2 and thrombin. Sepharose 11-20 fibrinogen beta chain Homo sapiens 0-10 3620475-0 1987 Fibrinogen-sepharose interaction with prothrombin, prethrombin 1, prethrombin 2 and thrombin. Sepharose 11-20 coagulation factor II, thrombin Homo sapiens 41-49 3620475-1 1987 Binding of prothrombin, prethrombin 1, prethrombin 2 and thrombin to fibrinogen-Sepharose was studied. Sepharose 80-89 fibrinogen beta chain Homo sapiens 69-79 3620475-2 1987 Thrombin and prethrombin 2 bound to fibrinogen-Sepharose, while prethrombin 1 and prothrombin did not. Sepharose 47-56 coagulation factor II, thrombin Homo sapiens 0-8 3620475-2 1987 Thrombin and prethrombin 2 bound to fibrinogen-Sepharose, while prethrombin 1 and prothrombin did not. Sepharose 47-56 fibrinogen beta chain Homo sapiens 36-46 3620475-4 1987 The affinity of thrombin and prethrombin 2 to fibrinogen-Sepharose depended on ionic strength and reached a maximum at 50 mm concentration. Sepharose 57-66 coagulation factor II, thrombin Homo sapiens 16-24 3620475-4 1987 The affinity of thrombin and prethrombin 2 to fibrinogen-Sepharose depended on ionic strength and reached a maximum at 50 mm concentration. Sepharose 57-66 fibrinogen beta chain Homo sapiens 46-56 3680409-2 1987 The interaction of lysozyme, ovalbumin, bovine and pig serum albumins with Cu2+ immobilized on Chelating Sepharose Fast Flow or TSK gel chelate-5PW was studied by frontal analysis at various initial concentrations of these solutes. Sepharose 105-114 lysozyme C, tracheal isozyme Bos taurus 19-27 2960319-4 1987 155, 1400-1411] purified a protein from Raji B-lymphoblastoid cell culture supernatants, using Factor H-Sepharose affinity chromatography. Sepharose 104-113 complement factor H Homo sapiens 95-103 2960319-11 1987 Examination of culture supernatants from biosynthetically radiolabelled Raji cells showed again the presence of a single soluble species that bound to Factor H-Sepharose, but this species was of lower Mr (approx. Sepharose 160-169 complement factor H Homo sapiens 151-159 2960320-2 1987 Smooth-muscle myosin purified as described by Persechini & Hartshorne [(1983) Biochemistry 22, 470-476] contains trace amounts of calmodulin and myosin light-chain kinase, which can be removed by Ca2+-dependent hydrophobic-interaction chromatography followed by calmodulin-Sepharose affinity chromatography. Sepharose 277-286 myosin heavy chain 14 Homo sapiens 14-20 2960320-2 1987 Smooth-muscle myosin purified as described by Persechini & Hartshorne [(1983) Biochemistry 22, 470-476] contains trace amounts of calmodulin and myosin light-chain kinase, which can be removed by Ca2+-dependent hydrophobic-interaction chromatography followed by calmodulin-Sepharose affinity chromatography. Sepharose 277-286 myosin light chain kinase Homo sapiens 149-174 3301850-1 1987 Seven distinct heparin-binding sites have been demonstrated on human apolipoprotein (apo) B-100 by using a combination of digestion with cyanogen bromide or Staphylococcus aureus V-8 protease and heparin-Sepharose affinity chromatography. Sepharose 204-213 apolipoprotein B Homo sapiens 69-95 2445044-2 1987 Kallikrein-C-1-Inh or factor XIIa-C-1-Inh complexes were bound to Sepharose to which monospecific antibodies against (pre)kallikrein or factor XII, respectively, were coupled. Sepharose 66-75 kallikrein related peptidase 4 Homo sapiens 0-10 2445044-2 1987 Kallikrein-C-1-Inh or factor XIIa-C-1-Inh complexes were bound to Sepharose to which monospecific antibodies against (pre)kallikrein or factor XII, respectively, were coupled. Sepharose 66-75 heterogeneous nuclear ribonucleoprotein C Homo sapiens 11-18 2445044-2 1987 Kallikrein-C-1-Inh or factor XIIa-C-1-Inh complexes were bound to Sepharose to which monospecific antibodies against (pre)kallikrein or factor XII, respectively, were coupled. Sepharose 66-75 heterogeneous nuclear ribonucleoprotein C Homo sapiens 34-41 2445044-2 1987 Kallikrein-C-1-Inh or factor XIIa-C-1-Inh complexes were bound to Sepharose to which monospecific antibodies against (pre)kallikrein or factor XII, respectively, were coupled. Sepharose 66-75 kallikrein related peptidase 4 Homo sapiens 122-132 3677181-2 1987 Its tryptic peptide pattern on reversed-phase HPLC was very similar to that of beta + gamma-actin, an actin sample prepared by affinity chromatography on DNase I-Sepharose contained the acidic polypeptide, and monoclonal anti-actin antibody reacted with it; therefore, the polypeptide is considered an actin isoform. Sepharose 162-171 deoxyribonuclease I Mus musculus 154-161 3689310-2 1987 Adenylosuccinate lyase from rat skeletal muscle was purified to apparent homogeneity by a combination of ion-exchange chromatography and affinity chromatography on agarose containing covalently bound adenylophosphonopropionate. Sepharose 164-171 adenylosuccinate lyase Rattus norvegicus 0-22 3622383-2 1987 Three of these proteins were also detected by affinity chromatography on calmodulin-Sepharose. Sepharose 84-93 calmodulin Bos taurus 73-83 3114144-1 1987 Granulocyte-macrophage colony-stimulating factor (GM-CSF) produced by splenic lymphocytes obtained from Schistosoma japonicum-infected mice was partially purified by a combination of DEAE anion-exchange chromatography, concanavalin A-Sepharose affinity chromatography, and high-pressure liquid chromatography. Sepharose 234-243 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 50-56 3429445-1 1987 The nontransformed glucocorticoid receptor (GR) and an 88-kDa protein in rat liver cytosol were selectively adsorbed on protamine- and arginine-Sepharose. Sepharose 144-153 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 19-42 3429445-1 1987 The nontransformed glucocorticoid receptor (GR) and an 88-kDa protein in rat liver cytosol were selectively adsorbed on protamine- and arginine-Sepharose. Sepharose 144-153 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 44-46 2822510-1 1987 The effect of a polyclonal anti-insulin receptor antibody (pIgG) on the insulin receptor tyrosine kinase (IRTK) activity toward poly-(Glu-Tyr) was examined using wheat germ agglutinin agarose-purified insulin receptors from rat liver membranes. Sepharose 184-191 insulin receptor Rattus norvegicus 32-48 2822510-1 1987 The effect of a polyclonal anti-insulin receptor antibody (pIgG) on the insulin receptor tyrosine kinase (IRTK) activity toward poly-(Glu-Tyr) was examined using wheat germ agglutinin agarose-purified insulin receptors from rat liver membranes. Sepharose 184-191 phosphatidylinositol glycan anchor biosynthesis, class G Rattus norvegicus 59-63 2822510-1 1987 The effect of a polyclonal anti-insulin receptor antibody (pIgG) on the insulin receptor tyrosine kinase (IRTK) activity toward poly-(Glu-Tyr) was examined using wheat germ agglutinin agarose-purified insulin receptors from rat liver membranes. Sepharose 184-191 insulin receptor Rattus norvegicus 72-88 2442667-1 1987 Migration properties and occurrence of antibodies against myelin basic protein (MBP) in paired CSF and serum specimens from patients with multiple sclerosis (MS) were demonstrated after agarose isoelectric focusing, immunoblot transfer, and immunoperoxidase staining. Sepharose 186-193 myelin basic protein Homo sapiens 80-83 2447894-1 1987 A specific antibody population against human fibrinogen was isolated from a rabbit antiserum by affinity chromatography on fibrinogen-bound Sepharose gel. Sepharose 140-149 fibrinogen beta chain Homo sapiens 123-133 3663509-7 1987 AT III from the patient was reapplied to heparin-Sepharose and eluted with a NaCl gradient. Sepharose 49-58 serpin family C member 1 Homo sapiens 0-6 2885397-9 1987 CAP2 kinase activity was separated from the coated vesicle membrane and from dis-assembled clathrin triskelions, coeluting with the assembly polypeptide complex on a Sepharose 4B column. Sepharose 166-175 cyclase associated actin cytoskeleton regulatory protein 2 Bos taurus 0-4 3040771-2 1987 Sepharose beads coupled to either monoclonal anti-gelsolin antibodies or to polyproline were used to extract gelsolin and profilin, respectively, from EGTA-containing platelet extracts and determine the proportion of these molecules bound to actin with sufficient affinity to withstand dilution (high-affinity complexes). Sepharose 0-9 gelsolin Homo sapiens 109-117 3110278-2 1987 Sepharose-immobilized antibody reactive with T3 induced the proliferation of resting T lymphocytes in the presence of either recombinant interleukin 2 or phorbol myristate acetate. Sepharose 0-9 interleukin 2 Homo sapiens 137-150 2442830-4 1987 Study of the high affinity binding of the xylan sulfates to AT-III-Sepharose column showed that the amount of the xylan sulfate recovered in the eluates from this peak was greatly increased with an increase in molecular weight (MW). Sepharose 67-76 serpin family C member 1 Homo sapiens 60-66 3039336-4 1987 DBH was purified from bovine adrenal medullae chromaffin vesicles by ion exchange, size exclusion, and concanavalin A-Sepharose chromatography. Sepharose 118-127 dopamine beta-hydroxylase Bos taurus 0-3 2956950-1 1987 BALB/c mice were immunized with rIGF-II receptors purified from 18-54, SF cells by chromatography of solubilized receptors over agarose-immobilized rIGF-II. Sepharose 128-135 insulin-like growth factor 2 Rattus norvegicus 32-39 2956950-1 1987 BALB/c mice were immunized with rIGF-II receptors purified from 18-54, SF cells by chromatography of solubilized receptors over agarose-immobilized rIGF-II. Sepharose 128-135 insulin-like growth factor 2 Rattus norvegicus 148-155 3619892-4 1987 In brain c-aFGF represented 66% of the total mitogenic activity retained on the heparin-sepharose column and c-bFGF 34% while retina contained 16% of c-aFGF and 84% of c-bFGF; vitreous 78% of c-aFGF and 22% of c-bFGF. Sepharose 88-97 fibroblast growth factor 1 Homo sapiens 11-15 3304287-2 1987 Using a SH-free glutathione-agarose column it is demonstrated that the interaction of insulin with glutathione is specific, and increasing the incubation time between these two peptides results in the reduction of insulin disulfide bonds and the production of A and B chains. Sepharose 28-35 insulin Homo sapiens 86-93 3304287-2 1987 Using a SH-free glutathione-agarose column it is demonstrated that the interaction of insulin with glutathione is specific, and increasing the incubation time between these two peptides results in the reduction of insulin disulfide bonds and the production of A and B chains. Sepharose 28-35 insulin Homo sapiens 214-221 3609301-5 1987 Chromatography of total tryptic digests on concanavalin A-Sepharose confirmed that the high heparin affinity form of antithrombin lacked an oligosaccharide moiety at Asn 135. Sepharose 58-67 serpin family C member 1 Homo sapiens 117-129 3606618-3 1987 The S6 kinase was further purified by chromatography on peptide R1A13-Sepharose 4B and Sephadex G-150. Sepharose 70-82 ribosomal protein S6 kinase B1 Rattus norvegicus 4-13 2954960-5 1987 To get more detailed information about this interaction, calmodulin-binding fragments were isolated from a CNBr digest of phosphofructokinase using affinity chromatography on calmodulin-agarose. Sepharose 186-193 calmodulin 1 Homo sapiens 57-67 2439589-11 1987 This property of responder sera was due to IgE because both heating sera at 56 degrees C for 2 hr and passage of sera over anti-IgE-Sepharose (which removes greater than 90% of the IgE) markedly reduced the ability of sera to induce responsiveness, and because an excess of either purified IgE myeloma or purified penicillin-specific IgE antibody from a nonresponder competitively inhibited the ability of IgE from responder sera to induce responsiveness to HRF. Sepharose 132-141 tumor protein, translationally-controlled 1 Homo sapiens 458-461 2954960-5 1987 To get more detailed information about this interaction, calmodulin-binding fragments were isolated from a CNBr digest of phosphofructokinase using affinity chromatography on calmodulin-agarose. Sepharose 186-193 calmodulin 1 Homo sapiens 175-185 3297790-1 1987 Ca2+-dependent chromatography of soluble cytosolic proteins on calmodulin-Sepharose gave a fraction that exhibited Ca2+- and calmodulin-dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. Sepharose 74-83 calmodulin Saccharomyces cerevisiae S288C 63-73 3297790-1 1987 Ca2+-dependent chromatography of soluble cytosolic proteins on calmodulin-Sepharose gave a fraction that exhibited Ca2+- and calmodulin-dependent phosphorylation of several polypeptides, including 60, 56 and 45 kDa species. Sepharose 74-83 calmodulin Saccharomyces cerevisiae S288C 115-135 3663598-1 1987 A calmodulin-sensitive adenylate cyclase has been purified to apparent homogeneity from bovine cerebral cortex using calmodulin-Sepharose followed by forskolin-Sepharose and wheat germ agglutinin-Sepharose. Sepharose 128-137 CaM5 Triticum aestivum 2-12 3663598-1 1987 A calmodulin-sensitive adenylate cyclase has been purified to apparent homogeneity from bovine cerebral cortex using calmodulin-Sepharose followed by forskolin-Sepharose and wheat germ agglutinin-Sepharose. Sepharose 128-137 calmodulin Bos taurus 117-127 3663598-1 1987 A calmodulin-sensitive adenylate cyclase has been purified to apparent homogeneity from bovine cerebral cortex using calmodulin-Sepharose followed by forskolin-Sepharose and wheat germ agglutinin-Sepharose. Sepharose 160-169 CaM5 Triticum aestivum 2-12 3663598-1 1987 A calmodulin-sensitive adenylate cyclase has been purified to apparent homogeneity from bovine cerebral cortex using calmodulin-Sepharose followed by forskolin-Sepharose and wheat germ agglutinin-Sepharose. Sepharose 160-169 CaM5 Triticum aestivum 2-12 3663598-3 1987 This polypeptide was a major component of the protein purified through calmodulin-Sepharose. Sepharose 82-91 CaM5 Triticum aestivum 71-81 3606467-4 1987 After separation of plasma lipoproteins by 4% agarose chromatography, an increased mass of apo E in lipoproteins of intermediate size was present; this may reflect the absence of LDL receptors that normally mediate their clearance. Sepharose 46-53 apolipoprotein E Homo sapiens 91-96 3037009-0 1987 Analysis of the bond between encephalomyocarditis virus and its human erythrocyte receptor by affinity chromatography on virus-sepharose columns. Sepharose 127-136 CD2 molecule Homo sapiens 70-90 3117099-1 1987 Bleomycin (BLM) hydrolase, a protective enzyme that inactivates the antitumor antibiotic BLM, was purified (6000-fold) to homogeneity from rabbit lungs by DEAE-Sephacel, phenyl-Sepharose chromatography, BLM-Sepharose affinity chromatography, and Mono Q fast protein liquid chromatography. Sepharose 177-186 bleomycin hydrolase Oryctolagus cuniculus 0-25 2885032-1 1987 Dipeptidyl peptidase IV (EC 3.4.14.5) was solubilized from rat liver plasma membranes with sulphobetaine 14 and purified by successive affinity chromatography on Con A-Sepharose, wheat germ lectin-Sepharose and arginine-Sepharose columns. Sepharose 197-206 dipeptidylpeptidase 4 Rattus norvegicus 0-23 3110143-5 1987 Fractionation of the labeled antithrombin by affinity chromatography on heparin-Sepharose separated a phosphopyridoxylated antithrombin species devoid of heparin binding from modified protein which retained affinity for heparin. Sepharose 80-89 serpin family C member 1 Homo sapiens 29-41 3110143-5 1987 Fractionation of the labeled antithrombin by affinity chromatography on heparin-Sepharose separated a phosphopyridoxylated antithrombin species devoid of heparin binding from modified protein which retained affinity for heparin. Sepharose 80-89 serpin family C member 1 Homo sapiens 123-135 2438280-1 1987 Previous studies using calmodulin-Sepharose affinity chromatography have suggested that bovine brain may contain a mixture of calmodulin-sensitive and -insensitive adenylate cyclase activities (Wescott, K. R., La Porte, D. C., and Storm, D. R. (1979) Proc. Sepharose 34-43 calmodulin 1 Rattus norvegicus 23-33 3584143-3 1987 This lectin, which we shall refer to as SB-1 lectin, was isolated on the basis of its carbohydrate-binding activity (affinity chromatography on Sepharose column derivatized with N-caproyl-galactosamine) and its immunological cross-reactivity (immunoblotting with rabbit antibodies directed against seed soybean agglutinin (SBA]. Sepharose 144-153 LOW QUALITY PROTEIN: lectin Glycine max 5-11 3584143-3 1987 This lectin, which we shall refer to as SB-1 lectin, was isolated on the basis of its carbohydrate-binding activity (affinity chromatography on Sepharose column derivatized with N-caproyl-galactosamine) and its immunological cross-reactivity (immunoblotting with rabbit antibodies directed against seed soybean agglutinin (SBA]. Sepharose 144-153 LOW QUALITY PROTEIN: lectin Glycine max 45-51 3630861-1 1987 A new isolation procedure of rat C-reactive protein (CRP) by affinity chromatography on amino-coupled phosphorylcolamine-Sepharose is described. Sepharose 121-130 C-reactive protein Rattus norvegicus 53-56 3109322-1 1987 Dihydrofolate reductase (DHFR; EC 1.5.1.3) was purified to homogeneity from soybean seedlings by affinity chromatography on methotrexate-aminohexyl Sepharose, gel filtration on Ultrogel AcA-54, and Blue Sepharose chromatography. Sepharose 148-157 chalcone reductase CHR1 Glycine max 14-23 3109322-1 1987 Dihydrofolate reductase (DHFR; EC 1.5.1.3) was purified to homogeneity from soybean seedlings by affinity chromatography on methotrexate-aminohexyl Sepharose, gel filtration on Ultrogel AcA-54, and Blue Sepharose chromatography. Sepharose 148-157 dihydrofolate reductase Homo sapiens 25-29 3663126-2 1987 All these proteins, like EF-2, were selectively retained by a heparin-Sepharose column, which we used as an affinity-chromatography step. Sepharose 70-79 eukaryotic translation elongation factor 2 Homo sapiens 25-29 3107633-12 1987 Stimulation of r-gamma IFN- or r-IL 2-activated LGL with K562 TC or Sepharose-bound anti-FcR antibody decreased their cytolytic ability, with cells depleted of granules at the ultrastructural level. Sepharose 68-77 interleukin 2 Homo sapiens 33-37 2438163-5 1987 The fraction of soluble protein retained on calmodulin-Sepharose 4B columns in the presence of Ca2+ and eluted by EGTA is 0.7%. Sepharose 55-64 calmodulin-1 Sus scrofa 44-54 3610142-2 1987 A simple method of two-dimensional agarose gel electrophoresis (pH 5.4)-horizontal PAGE (pH 9.0) of plasma samples, followed by general protein staining, was reported as an alternative method for alpha 1B typing. Sepharose 35-42 alpha-1-B glycoprotein Homo sapiens 196-204 3305098-6 1987 Then, 50 microliter of anti-CBG-Sepharose 4B (immunoadsorbent) was added to each incubation mixture, followed by incubation for another 4 hours at 25 degrees C. After centrifugation, radioactivity of the precipitate was counted and the results were analyzed using Scatchard plot. Sepharose 32-44 serpin family A member 6 Homo sapiens 28-31 2884926-9 1987 Antibodies specific for the heavy and light chains of myeloperoxidase were isolated from antiserum by affinity chromatography employing Sepharose columns covalently bound to the heavy or light chains. Sepharose 136-145 myeloperoxidase Homo sapiens 54-69 3496043-3 1987 HMW hEGF bound poorly to concanavalin A-agarose, quite avidly to wheat germ lectin-agarose, and completely to phenyl boronate-agarose, suggesting that it was O-glycosylated. Sepharose 83-90 epidermal growth factor Homo sapiens 4-8 3496043-3 1987 HMW hEGF bound poorly to concanavalin A-agarose, quite avidly to wheat germ lectin-agarose, and completely to phenyl boronate-agarose, suggesting that it was O-glycosylated. Sepharose 83-90 epidermal growth factor Homo sapiens 4-8 3553193-3 1987 In pulse-chase experiments with [35S] methionine, labeled receptor species were separated into "active" and "inactive" forms by affinity chromatography on insulin-agarose and then were characterized and quantitated. Sepharose 163-170 insulin Homo sapiens 155-162 3032267-5 1987 Using heparin Sepharose chromatography and stepwise elution, a lipoprotein lipase enriched fraction was recovered with 2 M NaCl. Sepharose 14-23 lipoprotein lipase Rattus norvegicus 63-81 2952648-3 1987 P-57 exhibits higher affinity for calmodulin-Sepharose in the absence of free Ca2+ than in the presence of Ca2+ (Andreasen, T.J., Luetje, C.W., Heideman, W. & Storm, D.R. Sepharose 45-54 cyclin dependent kinase inhibitor 1C Homo sapiens 0-4 2952648-16 1987 CaM decreased the rate of phosphorylation of P-57 by protein kinase C, and phosphorylation prevented P-57 binding to calmodulin-Sepharose. Sepharose 128-137 cyclin dependent kinase inhibitor 1C Homo sapiens 45-49 2952648-16 1987 CaM decreased the rate of phosphorylation of P-57 by protein kinase C, and phosphorylation prevented P-57 binding to calmodulin-Sepharose. Sepharose 128-137 cyclin dependent kinase inhibitor 1C Homo sapiens 101-105 3106500-0 1987 Separation of human IgA1 and IgA2 using jacalin-agarose chromatography. Sepharose 48-55 immunoglobulin heavy constant alpha 1 Homo sapiens 20-24 3106500-1 1987 A lectin isolated from the tropical jackfruit, jacalin, previously reported to precipitate human immunoglobulin A (IgA), and conjugated to agarose was used to separate the two subclasses of IgA from secretions. Sepharose 139-146 CD79a molecule Homo sapiens 190-193 3106500-2 1987 Jacalin-agarose binds specifically to the D-galactose moiety of IgA1 but not to IgA2 which has a different carbohydrate content and structure. Sepharose 8-15 immunoglobulin heavy constant alpha 1 Homo sapiens 64-68 2953243-2 1987 beta-Endorphin was extracted through Sepharose-treated chromatography columns and assayed with a specific anti-beta-endorphin antibody. Sepharose 37-46 proopiomelanocortin Homo sapiens 0-14 3605604-2 1987 The latter is specifically complexed onto a T-2 agarose gel. Sepharose 48-55 solute carrier family 25 member 5 Homo sapiens 44-47 3105618-4 1987 Acidification or addition of lysine to plasma is also required for maximum immunoadsorption of plasma tPA antigen on anti-tPA-Ig-sepharose. Sepharose 129-138 chromosome 20 open reading frame 181 Homo sapiens 102-105 3105618-4 1987 Acidification or addition of lysine to plasma is also required for maximum immunoadsorption of plasma tPA antigen on anti-tPA-Ig-sepharose. Sepharose 129-138 chromosome 20 open reading frame 181 Homo sapiens 122-125 3105618-6 1987 The plasma tPA antigen isolated by immunoadsorption of either untreated plasma or acidified plasma on anti-tPA-Ig-sepharose consists mainly of a 100-kd plasminogen activator species as determined by fibrin-agar zymography. Sepharose 114-123 chromosome 20 open reading frame 181 Homo sapiens 11-14 3105618-6 1987 The plasma tPA antigen isolated by immunoadsorption of either untreated plasma or acidified plasma on anti-tPA-Ig-sepharose consists mainly of a 100-kd plasminogen activator species as determined by fibrin-agar zymography. Sepharose 114-123 chromosome 20 open reading frame 181 Homo sapiens 107-110 3105619-2 1987 Depletion of tissue plasminogen activator (tPA) antigen by immunoadsorption of human plasma with anti-tPA Ig Sepharose 4B leads to total loss of spontaneous fibrinolytic activity determined by lysis of a thrombin-induced clot. Sepharose 109-118 chromosome 20 open reading frame 181 Homo sapiens 13-41 3105619-2 1987 Depletion of tissue plasminogen activator (tPA) antigen by immunoadsorption of human plasma with anti-tPA Ig Sepharose 4B leads to total loss of spontaneous fibrinolytic activity determined by lysis of a thrombin-induced clot. Sepharose 109-118 chromosome 20 open reading frame 181 Homo sapiens 43-46 3105619-2 1987 Depletion of tissue plasminogen activator (tPA) antigen by immunoadsorption of human plasma with anti-tPA Ig Sepharose 4B leads to total loss of spontaneous fibrinolytic activity determined by lysis of a thrombin-induced clot. Sepharose 109-118 chromosome 20 open reading frame 181 Homo sapiens 102-105 3567355-4 1987 By heparin-Sepharose chromatography, AT-III Rouen was separated from the normal antithrombin on elution with increasing concentrations of NaCl. Sepharose 11-20 serpin family C member 1 Homo sapiens 37-43 2436816-7 1987 Removal of SAP from the culture fluid with rabbit anti-Mo SAP antibody or agarose beads abrogated the suppression. Sepharose 74-81 amyloid P component, serum Mus musculus 11-14 2953614-8 1987 C3b purified by anion-exchange fast protein liquid chromatography on a Mono Q column or eluted from a monoclonal anti-C3b-Sepharose retained its modulating activity, while native C3 or C3 fragments such as iC3b, C3c or C3d,g were ineffective. Sepharose 122-131 complement C3 Homo sapiens 0-3 2953614-8 1987 C3b purified by anion-exchange fast protein liquid chromatography on a Mono Q column or eluted from a monoclonal anti-C3b-Sepharose retained its modulating activity, while native C3 or C3 fragments such as iC3b, C3c or C3d,g were ineffective. Sepharose 122-131 complement C3 Homo sapiens 118-121 3569440-7 1987 Heat inactivation diminished inhibitors in sera samples, but affinity chromatography using wheat-germ lectin-Sepharose was most effective for extracting erythropoietin and reducing nonspecific effects. Sepharose 109-118 erythropoietin Homo sapiens 153-167 2440800-4 1987 Immunoglobulins from ovalbumin-immunized rabbits were isolated on a protein A Sepharose column and then fractionated on insoluble ovalbumin; the retained fraction displayed G0-S activity and showed indirect immunofluorescence, whilst the non-retained fraction was inactive. Sepharose 78-87 serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene Mus musculus 21-30 2435753-2 1987 High mol wt IGF-binding protein was highly purified by chromatography of Cohn IV-1 fraction of human plasma on Concanavalin A-Sepharose followed by chromatography on IGF-I-Sepharose. Sepharose 172-181 insulin like growth factor 1 Homo sapiens 166-171 2435753-4 1987 Both fractions obtained after IGF-I-Sepharose chromatography were capable of binding [125I]IGF-I and gave a single protein band of 79,000 mol wt, when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Coomassie blue staining), suggesting that the large mol wt species may be a dimer of identical or iso-mol wt subunits. Sepharose 36-45 insulin like growth factor 1 Homo sapiens 30-35 2435753-4 1987 Both fractions obtained after IGF-I-Sepharose chromatography were capable of binding [125I]IGF-I and gave a single protein band of 79,000 mol wt, when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Coomassie blue staining), suggesting that the large mol wt species may be a dimer of identical or iso-mol wt subunits. Sepharose 36-45 insulin like growth factor 1 Homo sapiens 91-96 2820951-9 1987 Cytochrome P-450 (pHP3) was solubilized from microsomal membranes of the transformed yeast cells and purified partially on an aminooctyl Sepharose column (specific content, about 6 nmol per mg of protein). Sepharose 137-146 cytochrome P-450 Oryctolagus cuniculus 0-16 2820951-9 1987 Cytochrome P-450 (pHP3) was solubilized from microsomal membranes of the transformed yeast cells and purified partially on an aminooctyl Sepharose column (specific content, about 6 nmol per mg of protein). Sepharose 137-146 cytochrome P450 2C14 Oryctolagus cuniculus 18-22 2438566-1 1987 Chromatographic studies were performed to measure myelin basic protein (MBP) interactions by covalently binding a number of different proteins to Sepharose and passing radioactive bovine MBP over these columns. Sepharose 146-155 myelin basic protein Bos taurus 50-70 2438566-1 1987 Chromatographic studies were performed to measure myelin basic protein (MBP) interactions by covalently binding a number of different proteins to Sepharose and passing radioactive bovine MBP over these columns. Sepharose 146-155 myelin basic protein Bos taurus 72-75 3111049-5 1987 Specific antibodies to the transcortin were isolated from the homogeneous fraction of IgG by affinity chromatography on transcortin-sepharose 4B. Sepharose 132-141 serpin family A member 6 Rattus norvegicus 27-38 3111049-5 1987 Specific antibodies to the transcortin were isolated from the homogeneous fraction of IgG by affinity chromatography on transcortin-sepharose 4B. Sepharose 132-141 serpin family A member 6 Rattus norvegicus 120-131 3603728-1 1987 A commercial preparation of water-soluble acetylcholinesterase from horse red cells has been purified to a specific activity of 2380 U/mg of protein (a 1660-fold purification) by a twofold affinity chromatography on the known sorbent of Sepharose-p-[NH-(CH2)5-C(O)NH(CH2)5C(O)NH-]-C6H4-N+(CH3)3 X Br- at pH 7.5. Sepharose 237-246 acetylcholinesterase Equus caballus 42-62 2820385-15 1987 The Ca2+-calmodulin-sensitive phosphodiesterase from the DEAE-Sephacel column can be adsorbed to a calmodulin-Sepharose affinity column and eluted with EGTA. Sepharose 110-119 calmodulin 1 Homo sapiens 9-19 2820385-15 1987 The Ca2+-calmodulin-sensitive phosphodiesterase from the DEAE-Sephacel column can be adsorbed to a calmodulin-Sepharose affinity column and eluted with EGTA. Sepharose 110-119 calmodulin 1 Homo sapiens 99-109 2952732-2 1987 Rat IgG2a was used as the model of a low affinity ligand by serving as an inhibitor of the interaction between IgG-Sepharose and the receptors for IgE. Sepharose 115-124 gamma-2a immunoglobulin heavy chain Rattus norvegicus 4-9 3030445-1 1987 A collagenase inhibitor was purified from bovine cartilage by a combination of gel filtration, ion exchange, concanavalin A-Sepharose affinity chromatography, and elution from preparative sodium dodecyl sulfate-polyacrylamide gels. Sepharose 124-133 TIMP metallopeptidase inhibitor 2 Bos taurus 2-23 3558391-4 1987 A further increase is found when ConA-Sepharose-purified rhodopsin is used as the source of both "enzyme" and acceptor. Sepharose 38-47 CONA Bos taurus 33-37 3558391-4 1987 A further increase is found when ConA-Sepharose-purified rhodopsin is used as the source of both "enzyme" and acceptor. Sepharose 38-47 rhodopsin Bos taurus 57-66 2435798-10 1987 The induction of IL 3 production by suboptimal doses of either Con A or plastic-adsorbed F23.1 was inhibited by the anti-L3T4 antibody GK1.5, as was the response to F23.1 coupled to Sepharose-4B beads. Sepharose 182-191 interleukin 3 Mus musculus 17-21 3104329-8 1987 TRH caused a 2-fold increase in secretion of [3H]mannose-labeled TSH glycopeptides due almost exclusively to a specific increase in structures that bound to ConA-Sepharose and eluted with 10mM alpha-methylglucoside, corresponding to biantennary complex or unusual hybrid species. Sepharose 162-171 thyrotropin releasing hormone Mus musculus 0-3 3104329-11 1987 Moreover, ConA-Sepharose chromatography of secreted [3H]glucosamine- and [3H]fucose-labeled TSH glycopeptides showed similar increases in ConA-Sepharose binding with TRH as noted with [3H]mannose labeling. Sepharose 15-24 thyrotropin releasing hormone Mus musculus 166-169 3104329-11 1987 Moreover, ConA-Sepharose chromatography of secreted [3H]glucosamine- and [3H]fucose-labeled TSH glycopeptides showed similar increases in ConA-Sepharose binding with TRH as noted with [3H]mannose labeling. Sepharose 143-152 thyrotropin releasing hormone Mus musculus 166-169 2435719-4 1987 The electrophoretic mobility in agarose gels of human and rat alpha 2M is increased by 1 mol of trypsin, while the mobility of alpha 1M and alpha 1I3 is decreased. Sepharose 32-39 alpha-2-macroglobulin Rattus norvegicus 62-70 3104325-2 1987 The D2-dopamine receptor of the bovine anterior pituitary has been partially purified by affinity chromatography on CMOS-Sepharose (immobilized carboxymethyleneoximinospiperone). Sepharose 121-130 dopamine receptor D2 Bos taurus 4-24 3603409-6 1987 Affinity chromatography on heparin-Sepharose revealed two populations of AT III, one of which was devoid of heparin cofactor activity. Sepharose 35-44 serpin family C member 1 Homo sapiens 73-79 2882685-5 1987 Trypsin-activated renal inactive renin, purified on Cibacron blue agarose and pepstatin-amino-hexyl-Sepharose chromatography, was inhibited by pepstatin and by a renin inhibitor similarly to cat and human active renins. Sepharose 66-73 renin Homo sapiens 33-38 2882685-5 1987 Trypsin-activated renal inactive renin, purified on Cibacron blue agarose and pepstatin-amino-hexyl-Sepharose chromatography, was inhibited by pepstatin and by a renin inhibitor similarly to cat and human active renins. Sepharose 66-73 renin Homo sapiens 162-167 2882685-5 1987 Trypsin-activated renal inactive renin, purified on Cibacron blue agarose and pepstatin-amino-hexyl-Sepharose chromatography, was inhibited by pepstatin and by a renin inhibitor similarly to cat and human active renins. Sepharose 100-109 renin Homo sapiens 33-38 2882685-5 1987 Trypsin-activated renal inactive renin, purified on Cibacron blue agarose and pepstatin-amino-hexyl-Sepharose chromatography, was inhibited by pepstatin and by a renin inhibitor similarly to cat and human active renins. Sepharose 100-109 renin Homo sapiens 162-167 3111295-1 1987 A stable T-2 hydrazide gel is prepared by activating T-2 toxin with tresyl chloride followed by coupling to agarose-adipic acid hydrazide. Sepharose 108-115 solute carrier family 25 member 5 Homo sapiens 9-12 3300414-3 1987 The first step alone, heparin-agarose chromatography, is sufficient to purify the enzyme from yeast bearing a cloned copy of the ADE3 gene that overexpresses the protein. Sepharose 30-37 trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3 Saccharomyces cerevisiae S288C 129-133 3606568-1 1987 Human angiotensinogen has been purified 390-fold from serum by a rapid high-yielding procedure that involved chromatography on Blue Sepharose, phenyl-Sepharose, hydroxyapatite and immobilized 5-hydroxytryptamine (5-HT). Sepharose 132-141 angiotensinogen Homo sapiens 6-21 3606717-2 1987 LPL from post-heparin plasma was first purified by heparin Sepharose 4B affinity chromatography. Sepharose 59-71 lipoprotein lipase Homo sapiens 0-3 3030755-4 1987 This ribosomal protein S6 kinase activity was substantially purified by a combination of phosphocellulose, DEAE-cellulose, Mono Q and heparin-Sepharose chromatography, and some of its characteristics were examined. Sepharose 142-151 40S ribosomal protein S6 Mesocricetus auratus 5-25 3830178-4 1987 Positive clones were tested for their ability to bind phospholipase A2 in a specific immunoprecipitation assay involving protein-A--Sepharose to which rabbit anti-(mouse immunoglobulins) and monoclonal antibodies from hybridoma supernatants were complexed. Sepharose 132-141 phospholipase A2 group IB Rattus norvegicus 54-70 3549912-6 1987 Accurate determination of MIF became feasible by using cells from the human monocytoid cell line U937 as target cells in a microdroplet agarose assay. Sepharose 136-143 macrophage migration inhibitory factor Homo sapiens 26-29 3585104-1 1987 A NAD+-dependent 15-hydroxyprostaglandin dehydrogenase was purified from human term placenta using monoclonal antibody-coupled Sepharose affinity chromatography. Sepharose 127-136 15-hydroxyprostaglandin dehydrogenase Homo sapiens 2-54 3590112-1 1987 A study was performed to investigate if protamine covalently attached to CNBr-activated agarose is a useful tool for the purification of fibrinogen from human plasma samples. Sepharose 88-95 fibrinogen beta chain Homo sapiens 137-147 3036207-1 1987 Activated glucocorticoid receptor (GR) from the human cell line HeLa S3 was purified by differential chromatography on DNA-cellulose followed by DEAE-Sepharose chromatography to 50-60% homogeneity according to sodium dodecyl sulfate gel electrophoresis and densitometric scanning of silver-stained gels. Sepharose 150-159 nuclear receptor subfamily 3 group C member 1 Homo sapiens 10-33 3036207-1 1987 Activated glucocorticoid receptor (GR) from the human cell line HeLa S3 was purified by differential chromatography on DNA-cellulose followed by DEAE-Sepharose chromatography to 50-60% homogeneity according to sodium dodecyl sulfate gel electrophoresis and densitometric scanning of silver-stained gels. Sepharose 150-159 nuclear receptor subfamily 3 group C member 1 Homo sapiens 35-37 2434506-7 1987 The antiserum was divided into two fractions by affinity chromatography on hCG-Sepharose. Sepharose 79-88 chorionic gonadotropin subunit beta 5 Homo sapiens 75-78 2434563-3 1987 To test this hypothesis, rabbit anti-human C3 IgG antibodies which bound to Sepharose-coupled rabbit anti-factor H IgG (anti-H-binding anti-C3) were separated by affinity chromatography. Sepharose 76-85 complement factor H Homo sapiens 106-114 3593282-2 1987 Fibrin-monomer-Sepharose was used to study thrombin binding to fibrin and the role of the enzyme active centre in this interaction. Sepharose 15-24 coagulation factor II, thrombin Homo sapiens 43-51 3593282-5 1987 Also, the elution profile of phenylmethane-sulphonyl fluoride-inhibited thrombin from fibrinogen-Sepharose was identical with that of active thrombin from fibrin-monomer-Sepharose. Sepharose 97-106 coagulation factor II, thrombin Homo sapiens 72-80 3593282-5 1987 Also, the elution profile of phenylmethane-sulphonyl fluoride-inhibited thrombin from fibrinogen-Sepharose was identical with that of active thrombin from fibrin-monomer-Sepharose. Sepharose 97-106 fibrinogen beta chain Homo sapiens 86-96 3593282-5 1987 Also, the elution profile of phenylmethane-sulphonyl fluoride-inhibited thrombin from fibrinogen-Sepharose was identical with that of active thrombin from fibrin-monomer-Sepharose. Sepharose 170-179 coagulation factor II, thrombin Homo sapiens 72-80 3593282-5 1987 Also, the elution profile of phenylmethane-sulphonyl fluoride-inhibited thrombin from fibrinogen-Sepharose was identical with that of active thrombin from fibrin-monomer-Sepharose. Sepharose 170-179 coagulation factor II, thrombin Homo sapiens 141-149 3102483-4 1987 The complexes are of the appropriate size, bind to Sepharose that has been derivatized with anti-PNI antibody, do not form when the thrombin active site has been blocked with diisopropylphosphofluoridate, and do not appear on platelets when heparin is present. Sepharose 51-60 serpin family E member 2 Homo sapiens 97-100 2436920-9 1987 In peritoneal M phi activated in vivo, in addition to the 175-kDa band, new bands migrating at 140, 120 and 85 kDa were identified by 3A35 and could be absorbed on a commercial anti-T200 mAb bound to Sepharose beads. Sepharose 200-209 protein tyrosine phosphatase, receptor type, C Mus musculus 182-186 3612061-2 1987 The use of allo A-Sepharose for typing haptoglobin in bloodstains helped eliminate hemoglobin from the bloodstain extract and presented highly resolved haptoglobin patterns by disc gel electrophoresis. Sepharose 18-27 haptoglobin Homo sapiens 39-50 3612061-2 1987 The use of allo A-Sepharose for typing haptoglobin in bloodstains helped eliminate hemoglobin from the bloodstain extract and presented highly resolved haptoglobin patterns by disc gel electrophoresis. Sepharose 18-27 haptoglobin Homo sapiens 152-163 3142109-1 1987 Microsomal estrogen synthetase (aromatase) cytochrome P-450 was purified from fresh human placental microsomes by monoclonal anti-aromatase P-450 antibody-Sepharose 4B chromatography. Sepharose 155-164 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 54-59 3142109-1 1987 Microsomal estrogen synthetase (aromatase) cytochrome P-450 was purified from fresh human placental microsomes by monoclonal anti-aromatase P-450 antibody-Sepharose 4B chromatography. Sepharose 155-164 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 140-145 2954955-12 1987 The vasopressin receptor can be reconstituted from the N-acetylglucosamine-eluted peak of a wheat germ agglutinin-Sepharose column, and [3H] vasopressin binding activity is purified 5-6-fold from membranes by this chromatographic procedure. Sepharose 114-123 arginine vasopressin Rattus norvegicus 4-15 2954955-12 1987 The vasopressin receptor can be reconstituted from the N-acetylglucosamine-eluted peak of a wheat germ agglutinin-Sepharose column, and [3H] vasopressin binding activity is purified 5-6-fold from membranes by this chromatographic procedure. Sepharose 114-123 arginine vasopressin Rattus norvegicus 141-152 3036819-1 1987 A hemopexin receptor detected in detergent-solubilized placental membranes was purified from the human placenta, using hemopexin-Sepharose affinity chromatography. Sepharose 129-138 hemopexin Homo sapiens 2-11 3036819-1 1987 A hemopexin receptor detected in detergent-solubilized placental membranes was purified from the human placenta, using hemopexin-Sepharose affinity chromatography. Sepharose 129-138 hemopexin Homo sapiens 119-128 3038381-0 1987 Agarose gel isoelectrofocusing of UDP-galactose pyrophosphorylase and galactose-1-phosphate uridyltransferase. Sepharose 0-7 galactose-1-phosphate uridylyltransferase Homo sapiens 70-109 3597392-8 1987 Incubation of equine sperm adenylate cyclase with La3+ dissociated endogenous CaM from the enzyme so that most of the enzyme bound to a CaM-Sepharose column equilibrated with Ca2+. Sepharose 140-149 calmodulin-2 Equus caballus 136-139 3597392-9 1987 Specific elution of CaM-binding proteins from the CaM-Sepharose column with EGTA yielded a CaM-depleted adenylate cyclase fraction that was stimulated 2-fold by the addition of exogenous CaM. Sepharose 54-63 calmodulin-2 Equus caballus 20-23 3597392-9 1987 Specific elution of CaM-binding proteins from the CaM-Sepharose column with EGTA yielded a CaM-depleted adenylate cyclase fraction that was stimulated 2-fold by the addition of exogenous CaM. Sepharose 54-63 calmodulin-2 Equus caballus 50-53 3597392-9 1987 Specific elution of CaM-binding proteins from the CaM-Sepharose column with EGTA yielded a CaM-depleted adenylate cyclase fraction that was stimulated 2-fold by the addition of exogenous CaM. Sepharose 54-63 calmodulin-2 Equus caballus 50-53 3597392-9 1987 Specific elution of CaM-binding proteins from the CaM-Sepharose column with EGTA yielded a CaM-depleted adenylate cyclase fraction that was stimulated 2-fold by the addition of exogenous CaM. Sepharose 54-63 calmodulin-2 Equus caballus 50-53 2439124-3 1987 Immobilization of anti-peptide antibodies using agarose activated either with CNBr or with N-hydroxysuccinimido groups largely inactivated binding sites for erythropoietin. Sepharose 48-55 erythropoietin Homo sapiens 157-171 2885032-1 1987 Dipeptidyl peptidase IV (EC 3.4.14.5) was solubilized from rat liver plasma membranes with sulphobetaine 14 and purified by successive affinity chromatography on Con A-Sepharose, wheat germ lectin-Sepharose and arginine-Sepharose columns. Sepharose 168-177 dipeptidylpeptidase 4 Rattus norvegicus 0-23 2885032-1 1987 Dipeptidyl peptidase IV (EC 3.4.14.5) was solubilized from rat liver plasma membranes with sulphobetaine 14 and purified by successive affinity chromatography on Con A-Sepharose, wheat germ lectin-Sepharose and arginine-Sepharose columns. Sepharose 197-206 dipeptidylpeptidase 4 Rattus norvegicus 0-23 2953726-1 1987 A cell-surface fibronectin receptor was isolated from primary rat hepatocytes by affinity chromatography on Sepharose conjugated with the cell-binding domain (105 kDa) of fibronectin. Sepharose 108-117 fibronectin 1 Rattus norvegicus 15-26 3584127-4 1987 Affinity fractionation of HNB-labeled antithrombin (0.6-0.7 mol of HNB/mol of protein) on heparin-Sepharose using a linear salt gradient allowed separation of three singly labeled protein species and a fourth HNB-antithrombin species which co-eluted with unlabeled protein. Sepharose 98-107 serpin family C member 1 Homo sapiens 38-50 3630861-1 1987 A new isolation procedure of rat C-reactive protein (CRP) by affinity chromatography on amino-coupled phosphorylcolamine-Sepharose is described. Sepharose 121-130 C-reactive protein Rattus norvegicus 33-51 2820320-9 1987 Immune complexes in PMR and GCA differed from those previously characterized in rheumatoid arthritis (RA)1 purified by anti-C1q-Sepharose which contained immunoglobulins and C1q only. Sepharose 128-137 complement C1q A chain Homo sapiens 124-127 3661987-2 1987 A 900- to 1700-fold purification of the estrogen receptor was obtained using ammonium sulfate precipitation followed by dye affinity chromatography with Reactive Orange 14 immobilized to Sepharose. Sepharose 187-196 estrogen receptor 1 Bos taurus 40-57 3661987-5 1987 The Reactive Orange 14-Sepharose is easily prepared and offers a higher yield and purity of the estrogen receptor than that afforded by estrogen- or heparin-Sepharose chromatography. Sepharose 23-32 estrogen receptor 1 Bos taurus 96-113 2955807-2 1987 The abnormal species were purified using affinity chromatography on Sepharose bound anti-AT III antibodies. Sepharose 68-77 serpin family C member 1 Homo sapiens 89-95 3471320-2 1987 TGF beta, at low concentrations (between 0.1 and 1.0 ng/ml), inhibited the proliferation of MOSER cells both in monolayer culture and soft agarose, in a dose-dependent manner. Sepharose 139-146 transforming growth factor beta 1 Homo sapiens 0-8 3568326-2 1987 The formation of factor XIIIa-catalyzed fibrin polymers during clotting of plasma and purified fibrinogen in vivo was followed by a sodium dodecyl sulfate agarose gel technique, and an increase in both amount and size of gamma-chain cross-linked polymers was demonstrated before visible clot formation. Sepharose 155-162 coagulation factor XIII A chain Homo sapiens 17-29 3568326-2 1987 The formation of factor XIIIa-catalyzed fibrin polymers during clotting of plasma and purified fibrinogen in vivo was followed by a sodium dodecyl sulfate agarose gel technique, and an increase in both amount and size of gamma-chain cross-linked polymers was demonstrated before visible clot formation. Sepharose 155-162 fibrinogen beta chain Homo sapiens 95-105 3594828-0 1987 Isoelectric focusing of neuraminidase-treated alkaline phosphatase isoenzymes on agarose gel. Sepharose 81-88 neuraminidase 1 Homo sapiens 24-37 3296960-11 1987 Determination of the molecular weight of DU-PAN-2 in serum from a pancreatic cancer patient by Sepharose 4B gel filtration demonstrated the existence as a high-molecular-weight protein between about M.W. Sepharose 95-107 poly(A) specific ribonuclease subunit PAN2 Homo sapiens 44-49 3115913-1 1987 We demonstrate the possibility of a complete routine study on unconcentrated CSF from patients with Multiple Sclerosis (MS) using agarose isoelectric focusing (AIEF) followed by avidin-biotin-peroxidase complex staining to detect IgG oligoclonal bands (OB) and free light chains (FLC). Sepharose 130-137 colony stimulating factor 2 Homo sapiens 77-80 3654180-4 1987 Polyacrylamide isoelectric focusing appeared more sensitive than agarose electrophoresis for the detection of abnormal CSF fractions. Sepharose 65-72 colony stimulating factor 2 Homo sapiens 119-122 16665451-2 1987 The Apase-1(1) electrophoretic variant has been purified from a tomato cell suspension culture using ion exchange and concanavalin A sepharose affinity chromatography. Sepharose 133-142 acid phosphatase 1 Solanum lycopersicum 4-14 3036115-0 1987 Purification of 5"-nucleotidase from human placenta after release from plasma membranes by phosphatidylinositol-specific phospholipase C. 5"-Nucleotidase was purified greater than 1000-fold from human placenta by treatment of plasma membranes with S. aureus phosphatidylinositol-specific phospholipase C and affinity chromatography on Con A Sepharose and AMP-Sepharose. Sepharose 341-350 5'-nucleotidase ecto Homo sapiens 138-153 3036115-0 1987 Purification of 5"-nucleotidase from human placenta after release from plasma membranes by phosphatidylinositol-specific phospholipase C. 5"-Nucleotidase was purified greater than 1000-fold from human placenta by treatment of plasma membranes with S. aureus phosphatidylinositol-specific phospholipase C and affinity chromatography on Con A Sepharose and AMP-Sepharose. Sepharose 359-368 5'-nucleotidase ecto Homo sapiens 138-153 3619018-0 1987 Determination of apolipoprotein variants by isoelectric focusing in agarose. Sepharose 68-75 apolipoprotein E Homo sapiens 17-31 3619018-2 1987 In this paper we describe the use of agarose/urea as a focusing medium for IEF of apolipoproteins. Sepharose 37-44 apolipoprotein E Homo sapiens 82-97 2952119-3 1987 It was partially purified by affinity chromatography heparin-agarose column and was shown to be a serine protease. Sepharose 61-68 kallikrein 1-related peptidase C8 Rattus norvegicus 98-113 3546304-3 1987 Rat platelet factor 4 was specifically isolated and characterized by its high affinity for heparin-Sepharose and its amino-terminal sequence homology to human and rabbit platelet factor 4. Sepharose 99-108 platelet factor 4 Rattus norvegicus 4-21 3028536-3 1987 During the third trimester of pregnancy, her platelet counts dropped to 20,000 to 30,000/microL, and an increase in the intermediate-sized vWF multimers was seen on agarose gel electrophoresis. Sepharose 165-172 von Willebrand factor Homo sapiens 139-142 3494653-5 1987 The androgen receptor was also partially purified using heparin-sepharose chromatography The involvement of the anddrogen receptor in the mode of action of trenbolone is discussed. Sepharose 64-73 androgen receptor Ovis aries 4-21 3549614-2 1987 Purification of S-ag was readily achieved by affinity chromatography using four different MoAb-Sepharose 4B columns. Sepharose 95-104 S-antigen visual arrestin Homo sapiens 16-20 3549614-4 1987 The use of two different MoAbs covalently bound to Sepharose 4B and known to be directed to disparate, spacially distant epitopes on S-ag led to at least a twofold increase in recovery, with the aforementioned purity. Sepharose 51-60 S-antigen visual arrestin Homo sapiens 133-137 3597343-2 1987 Phospholipase A2 released from rat platelets was purified by the sequential use of column chromatography on heparin-Sepharose and TSK gel G2000SW (high-performance liquid chromatography, HPLC). Sepharose 116-125 phospholipase A2 group IB Rattus norvegicus 0-16 3597343-6 1987 Lysophospholipase, which was also released from rat platelets, was separated from phospholipase A2 by chromatography on heparin-Sepharose. Sepharose 128-137 asparaginase Rattus norvegicus 0-17 3037009-7 1987 Treatment of glycophorin with neuraminidase prevented it binding to EMC virus-Sepharose indicating the requirement for sialic acid for receptor activity. Sepharose 78-87 neuraminidase 1 Homo sapiens 30-43 3031189-4 1987 Following this receptor-mediated interaction, recombinant TNF-alpha was found to inhibit the migration of PMNs under agarose and to enhance PMN production of superoxide anion (O-2) in a dose-dependent manner. Sepharose 117-124 tumor necrosis factor Homo sapiens 58-67 3546290-11 1987 pRa of amniotic fluid and plasma could be separated from R by affinity chromatography on Cibacron blue F3GA-agarose, and R but not pRa was detected by an immunoassay using monoclonal antibodies reacting with R and not with pR. Sepharose 108-115 S100 calcium binding protein A6 Homo sapiens 0-3 3572257-5 1987 The reconstituted DMPC-containing HDL3 (DMPC-r-HDL3) was similar to native HDL3 and to C-r-HDL3 in its agarose gel electrophoretic mobility, in its chemical composition, and in its binding to rat liver plasma membranes. Sepharose 103-110 HDL3 Homo sapiens 34-38 3586653-11 1987 We therefore believe that hexynyl-nortestosterone-Sepharose, having a high density of a high affinity ligand, and having chemically and biochemically stable covalent bonds, should be a good reagent for affinity purification of PgR. Sepharose 50-59 progesterone receptor Homo sapiens 227-230 2434491-6 1987 The 38-kDa fragment, purified by high performance liquid chromatography, and several of its subfragments at 21 and 25 kDa sediment with F-actin, bind to calmodulin-Sepharose in the presence of Ca2+, and are displaced from F-actin by Ca2+-calmodulin. Sepharose 164-173 calmodulin 2 Gallus gallus 153-163 2434491-6 1987 The 38-kDa fragment, purified by high performance liquid chromatography, and several of its subfragments at 21 and 25 kDa sediment with F-actin, bind to calmodulin-Sepharose in the presence of Ca2+, and are displaced from F-actin by Ca2+-calmodulin. Sepharose 164-173 calmodulin 2 Gallus gallus 238-248 2434491-12 1987 The smallest subfragment of this peptide that binds to both F-actin and calmodulin-Sepharose is about 21 kDa. Sepharose 83-92 calmodulin 2 Gallus gallus 72-82 2434549-8 1987 The enhancing activities of TCS on IgE synthesis and on histamine release could be removed by absorption with IgE-Sepharose and subsequently recovered by elution with glycine buffer. Sepharose 114-123 immunoglobulin heavy constant epsilon Homo sapiens 35-38 2434549-8 1987 The enhancing activities of TCS on IgE synthesis and on histamine release could be removed by absorption with IgE-Sepharose and subsequently recovered by elution with glycine buffer. Sepharose 114-123 immunoglobulin heavy constant epsilon Homo sapiens 110-113 3105045-5 1987 SAP bound to TNP-agarose was eluated by either EDTA or p-nitrophenylarsonic acid. Sepharose 17-24 amyloid P component, serum Homo sapiens 0-3 3036083-7 1987 Affinity-labelled VIP-binding proteins solubilized by Nonidet P-40 bound to WGA-Sepharose and could be eluted specifically with N-acetyl-D-glucosamine. Sepharose 80-89 vasoactive intestinal peptide Homo sapiens 18-21 3029065-1 1987 The characteristics of the Ah receptor from rat liver were investigated following the incubation of cytosol with [3H]2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) under various conditions, and using DEAE- and DNA-Sepharose chromatography and sucrose density gradient centrifugation. Sepharose 210-219 aryl hydrocarbon receptor Rattus norvegicus 27-38 3818596-3 1987 In the final purification step, partially purified CETP is incubated with a synthetic lipid emulsion consisting of phosphatidylcholine, triglyceride, and fatty acid, and the bound activity, which elutes in the void volume, is separated from nonbound proteins by gel filtration on Sepharose 4B. Sepharose 280-289 cholesteryl ester transfer protein Homo sapiens 51-55 3818600-1 1987 Microsomal estrogen synthetase (cytochrome P-450ES), also known as aromatase, was purified from fresh human placenta microsomes by DEAE-Trisacryl and testosterone-agarose chromatography. Sepharose 163-170 cytochrome P450 family 19 subfamily A member 1 Homo sapiens 11-30 3567141-3 1987 Chromatography of irradiated calmodulin, using Ultrogel AcA 54 and phenyl-agarose columns, yields several distinctive fractions. Sepharose 74-81 calmodulin Oryctolagus cuniculus 29-39 2879844-7 1987 Guinea pig liver transglutaminase adsorbed well to GTP-agarose affinity columns, but not to CTP-agarose columns, and the binding was inhibited by the presence of calcium ions. Sepharose 55-62 protein-glutamine gamma-glutamyltransferase 2 Cavia porcellus 17-33 3468112-1 1987 Casein kinase II of yeast has been purified to near homogeneity by a procedure which includes affinity chromatography on heparin-agarose. Sepharose 129-136 casein kinase IIalpha Drosophila melanogaster 0-16 2434833-5 1987 Induction of interleukin 2 production by Jurkat cells and proliferation by normal T cells requires anti-CD3 stimulation with antibody on a solid support, such as Sepharose beads or a plastic dish. Sepharose 162-171 interleukin 2 Homo sapiens 13-26 3802545-3 1987 Protein A-Sepharose removed most of the LD activity from plasma but did not dissociate the complex, indicating that the Fab end of the Ig molecule was involved in binding. Sepharose 10-19 FA complementation group B Homo sapiens 120-123 2947901-3 1987 The affinity of binding was increased in the presence of tropomyosin, and this could be attributed to a direct interaction between caldesmon and tropomyosin which was demonstrated using caldesmon cross-linked to Sepharose. Sepharose 212-221 caldesmon 1 Homo sapiens 131-140 2947901-3 1987 The affinity of binding was increased in the presence of tropomyosin, and this could be attributed to a direct interaction between caldesmon and tropomyosin which was demonstrated using caldesmon cross-linked to Sepharose. Sepharose 212-221 caldesmon 1 Homo sapiens 186-195 3024734-1 1987 Myeloperoxidase and eosinophil peroxidase were separated and purified from rat bone marrow cells using cetyltrimethylammonium bromide as the solubilizer and then with column chromatographies on CM-Sephadex C-50 and Con A-Sepharose. Sepharose 221-230 myeloperoxidase Rattus norvegicus 0-15 3565747-4 1987 Then the haptoglobin-containing fractions are fractionated by negative immunoadsorption chromatography on anti-chicken hemoglobin-protein A-Sepharose to remove chicken hemoglobin-human haptoglobin complexes. Sepharose 140-149 haptoglobin Homo sapiens 9-20 3475025-0 1987 Interferometric determination of the effective diffusion coefficient of albumin in single sepharose beads. Sepharose 90-99 albumin Homo sapiens 72-79 3439853-2 1987 Increase in concentration of free AGP resulted in a release of the whole IgG-3 from AGP-Sepharose and a half of the bound IgG-3 from leukocyte surface. Sepharose 88-97 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 73-78 3817155-3 1987 Concanavalin A-Sepharose 4B chromatography revealed that the 25 kDa form was a glycosylated variant of PRL. Sepharose 15-24 prolactin Homo sapiens 103-106 3100052-2 1987 A synthetic oligonucleotide containing an hsp83-like heat shock element (HSE) was prepared and ligated into concatamers and covalently coupled to Sepharose. Sepharose 146-155 Heat shock protein 83 Drosophila melanogaster 42-47 2948956-2 1987 The binding of human SAP to heparan sulfate and dermatan sulfate was studied using Sepharose-immobilized SAP. Sepharose 83-92 amyloid P component, serum Homo sapiens 21-24 2948956-2 1987 The binding of human SAP to heparan sulfate and dermatan sulfate was studied using Sepharose-immobilized SAP. Sepharose 83-92 amyloid P component, serum Homo sapiens 105-108 3551665-5 1987 We have developed rapid purification procedures for ALDH1 and ALDH2 by use of agarose-AMP affinity chromatography and high-performance anion-exchange liquid chromatography (HPLC). Sepharose 78-85 aldehyde dehydrogenase 1 family member A1 Homo sapiens 52-57 3551665-5 1987 We have developed rapid purification procedures for ALDH1 and ALDH2 by use of agarose-AMP affinity chromatography and high-performance anion-exchange liquid chromatography (HPLC). Sepharose 78-85 aldehyde dehydrogenase 2 family member Homo sapiens 62-67 3826872-7 1987 Seemingly, agarose electrophoresis of CSF is a simple and reliable technique that aids in the diagnosis of CNS disorders of dogs. Sepharose 11-18 colony stimulating factor 2 Canis lupus familiaris 38-41 2948581-8 1987 From octylglucoside extracts of radioactively surface-labeled cells, distinct Mr 190,000/185,000 membrane glycoproteins bound to Fn-heptapeptide-Sepharose, further suggesting that the Mr 190,000 polypeptide would be the Fn-receptor of the K562 cells. Sepharose 145-154 fibronectin 1 Homo sapiens 129-131 3593220-9 1987 The high-Mr IGF-II complex bound to concanavalin A-Sepharose, suggesting that it was a glycoprotein. Sepharose 51-60 insulin like growth factor 2 Homo sapiens 12-18 3026617-3 1987 On the basis of these findings, we investigated the effect of exogenously added BN and GRP on the soft agarose colony growth of a panel of human cell lines. Sepharose 103-110 gastrin releasing peptide Homo sapiens 80-82 2437241-0 1987 [Detection of beta 2-transferrin with agarose gel electrophoresis, immunofixation and silver staining in cerebrospinal fluid, secretions and other body fluids]. Sepharose 38-45 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 14-20 2437241-0 1987 [Detection of beta 2-transferrin with agarose gel electrophoresis, immunofixation and silver staining in cerebrospinal fluid, secretions and other body fluids]. Sepharose 38-45 transferrin Homo sapiens 21-32 2437241-3 1987 The high sensitivity and specificity of the test depend on immunofixation of the electrophoretically separated transferrin in the agarose gel, and on the visualization of the immune complex by staining with alkaline silver nitrate solution. Sepharose 130-137 transferrin Homo sapiens 111-122 3027701-7 1987 Incubation of 2.5 units of crude DNA polymerase alpha with 4 units of agarose-immobilized alkaline phosphatase resulted in a nearly complete inhibition of DNA polymerase alpha activity. Sepharose 70-77 DNA polymerase alpha 1, catalytic subunit Homo sapiens 33-53 3027701-7 1987 Incubation of 2.5 units of crude DNA polymerase alpha with 4 units of agarose-immobilized alkaline phosphatase resulted in a nearly complete inhibition of DNA polymerase alpha activity. Sepharose 70-77 DNA polymerase alpha 1, catalytic subunit Homo sapiens 155-175 3029680-2 1987 In the chromatographic characteristics including the affinity to immobilized ATP the dnaB-like protein of P. aeruginosa is similar to the dnaB protein of E. coli with the exception that it does not bind to heparin-Sepharose. Sepharose 214-223 DnaB Pseudomonas aeruginosa 85-89 2432019-5 1987 Three antigens, CA 19-9, Mov 2, and DU-PAN-2, demonstrated size heterogeneity on agarose gel immunoblotting with their respective antibodies and gave different migration patterns from sera, bile, and pancreatic juice of patients with pancreatic adenocarcinoma. Sepharose 81-88 poly(A) specific ribonuclease subunit PAN2 Homo sapiens 39-44 3109378-1 1987 Human lactase was isolated from solubilized small-intestinal brush-border membranes by a combination of chromatography on concanavalin A-Sepharose, Bio-Gel 1.5m and chromatofocusing, with a yield of approx. Sepharose 137-146 lactase Homo sapiens 6-13 3109378-11 1987 This antibody population was used to set up an antibody-bound Sepharose column for the use in an immunoaffinity purification of lactase from crude intestinal homogenate. Sepharose 62-71 lactase Homo sapiens 128-135 3804998-2 1987 This factor is separated from eukaryotic initiation factor (eIF)-4B by chromatography on m7GTP-Sepharose. Sepharose 95-104 eukaryotic translation initiation factor 4B1 Triticum aestivum 30-67 3804998-3 1987 eIF-4B binds to m7GTP-Sepharose, whereas the stimulatory factor does not. Sepharose 22-31 eukaryotic translation initiation factor 4B1 Triticum aestivum 0-6 3539931-1 1987 Insulin-related factor (IRF), a polypeptide secreted by the mouse teratoma-derived cell line (1246-3A), was purified 3210-fold to homogeneity from 1246-3A conditioned medium using a rapid three-step procedure including cation-exchange chromatography, immunoaffinity chromatography using a monoclonal antibody against porcine insulin coupled to an agarose gel support, and reverse phase high performance liquid chromatography. Sepharose 347-354 insulin Bos taurus 0-7 3815073-2 1987 All 3 sites are solubilized by 3% Triton X-100, 1% Tween-80 and can be enriched by serotonin-linked-Sepharose 4B affinity chromatography. Sepharose 100-109 paired box 5 Homo sapiens 0-5 3492971-2 1987 High levels of aggregated IgG bearing C3d were found in RA synovial fluids, and IgG was the major immunoglobulin bound from such synovial fluids by anti-C3d Sepharose. Sepharose 157-166 endogenous retrovirus group K member 13 Homo sapiens 153-156 3492971-8 1987 In addition, the material bound by anti-C3d Sepharose from most synovial fluid polyethylene glycol precipitates did not contain either IgM or IgG RF. Sepharose 44-53 endogenous retrovirus group K member 13 Homo sapiens 40-43 3619643-1 1987 The cytochrome P-450 fraction of microsomes separated on lauric acid AH-Sepharose 4B columns contains about 75% of the microsomal P-450. Sepharose 72-81 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 4-20 3663046-0 1987 [Glutamate pyruvate transaminase (GPT): a new demonstration method using agarose electrophoresis]. Sepharose 73-80 glutamic--pyruvic transaminase Homo sapiens 34-37 2432967-4 1987 The appearance of proteins lacking biological specificity to lysin-sepharose in the plasminogen preparation shows the ability of activated plasminogen and plasmin to form complexes with these proteins and demonstrates the retention of the functional activity in lysin-binding regions on their molecules. Sepharose 67-76 plasminogen Homo sapiens 84-91 2886138-1 1987 The method for the preparation of a specific proteinase adsorbent (acid-resistant human urinary proteinase inhibitor--UPI--immobilized on sepharose) has been developed. Sepharose 138-147 endogenous retrovirus group K member 25 Homo sapiens 45-55 2886138-1 1987 The method for the preparation of a specific proteinase adsorbent (acid-resistant human urinary proteinase inhibitor--UPI--immobilized on sepharose) has been developed. Sepharose 138-147 endogenous retrovirus group K member 25 Homo sapiens 96-106 3040332-10 1987 Third, CR3 was isolated from solubilized neutrophils by affinity chromatography on beta-glucan-Sepharose. Sepharose 95-104 teratocarcinoma-derived growth factor 1 pseudogene 3 Homo sapiens 7-10 3311040-4 1987 The OC-MIF was purified about 10,000 fold by affinity chromatography on L-fucose-Sepharose 6B. Sepharose 81-90 macrophage migration inhibitory factor Homo sapiens 7-10 3311040-9 1987 This monoclonal antibody coupled to Sepharose 4B adsorbed OC-MIF. Sepharose 36-48 macrophage migration inhibitory factor Homo sapiens 61-64 2444387-5 1987 Of the nine antibodies three were non-precipitable but six could form clear visible precipitation lines with egg-white avidin in agarose gel. Sepharose 129-136 avidin Gallus gallus 119-125 3023028-5 1987 In a second series of studies, a GnRH analog (D-Lys6-GnRH) was immobilized to a cross-linked agarose matrix. Sepharose 93-100 gonadotropin releasing hormone 1 Homo sapiens 33-37 3427905-9 1987 Agarose gels containing yeast RNA substrate were used to monitor partial purification of the larval ribonuclease. Sepharose 0-7 ribonuclease Saccharomyces cerevisiae S288C 100-112 3621887-2 1987 Fibronectin purified from humans plasma by affinity chromatography on gelatin-agarose and heparin-agarose was cleaved by thrombin into a 29,000 Dalton and a 210,000 Dalton fragments. Sepharose 78-85 fibronectin 1 Homo sapiens 0-11 3621887-2 1987 Fibronectin purified from humans plasma by affinity chromatography on gelatin-agarose and heparin-agarose was cleaved by thrombin into a 29,000 Dalton and a 210,000 Dalton fragments. Sepharose 78-85 coagulation factor II, thrombin Homo sapiens 121-129 3621887-2 1987 Fibronectin purified from humans plasma by affinity chromatography on gelatin-agarose and heparin-agarose was cleaved by thrombin into a 29,000 Dalton and a 210,000 Dalton fragments. Sepharose 98-105 fibronectin 1 Homo sapiens 0-11 3621887-2 1987 Fibronectin purified from humans plasma by affinity chromatography on gelatin-agarose and heparin-agarose was cleaved by thrombin into a 29,000 Dalton and a 210,000 Dalton fragments. Sepharose 98-105 coagulation factor II, thrombin Homo sapiens 121-129 2946571-1 1987 Rat liver microsomal insulin-like growth factor-II (IGF-II) receptor has been purified to homogeneity using a single step affinity chromatographic procedure on agarose-IGF-II with elution at pH 4. Sepharose 160-167 insulin-like growth factor 2 Rattus norvegicus 21-68 2946571-1 1987 Rat liver microsomal insulin-like growth factor-II (IGF-II) receptor has been purified to homogeneity using a single step affinity chromatographic procedure on agarose-IGF-II with elution at pH 4. Sepharose 160-167 insulin-like growth factor 2 Rattus norvegicus 52-58 3023028-5 1987 In a second series of studies, a GnRH analog (D-Lys6-GnRH) was immobilized to a cross-linked agarose matrix. Sepharose 93-100 gonadotropin releasing hormone 1 Homo sapiens 53-57 3319844-2 1987 Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Sepharose 150-159 transferrin Sus scrofa 26-37 3319844-2 1987 Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Sepharose 150-159 transferrin Sus scrofa 90-101 3319844-2 1987 Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Sepharose 150-159 transferrin Sus scrofa 90-101 3817677-0 1987 Agarose gel electrophoresis of human erythrocyte glutamic-pyruvic transaminase (GPT EC 2.6.1.2). Sepharose 0-7 glutamic--pyruvic transaminase Homo sapiens 80-83 2828192-3 1987 Thirty hours after transfection, the secreted tPA was found in serum-free medium and measured by a fibrin-agarose plate assay (FAPA). Sepharose 106-113 chromosome 20 open reading frame 181 Homo sapiens 46-49 3497849-1 1987 A fast-migrating precipitin peak which showed a reaction of partial immunochemical identity with the major von Willebrand factor (vWf) component was detected by crossed immunoelectrophoresis in agarose gel in plasma but not in platelets from a patient with type IIA von Willebrand"s disease (vWD). Sepharose 194-201 von Willebrand factor Homo sapiens 107-128 3114102-3 1987 Crossed immunoelectrophoresis of AT III on heparin-agarose was also carried out in plasma and serum. Sepharose 51-58 serpin family C member 1 Homo sapiens 33-39 3114102-7 1987 In all groups all serum AT III parameters were higher than in controls; crossed immunoelectrophoresis of AT III on heparin-agarose indicated that this finding was due to a lower formation of complexed AT III in serum. Sepharose 123-130 serpin family C member 1 Homo sapiens 105-111 3114102-7 1987 In all groups all serum AT III parameters were higher than in controls; crossed immunoelectrophoresis of AT III on heparin-agarose indicated that this finding was due to a lower formation of complexed AT III in serum. Sepharose 123-130 serpin family C member 1 Homo sapiens 105-111 3569638-3 1987 Vitellogenin mRNA was isolated from estrogenized chick liver, fractionated by electrophoresis using formaldehyde/agarose gels and blot transferred to nitrocellulose paper. Sepharose 113-120 vitellogenin 2 Gallus gallus 0-12 2447388-7 1987 The rat liver glucocorticoid receptor also interacts with high affinity with 2G8 antiphosphotyrosine antibody coupled to Sepharose (Kd value of 0.21 nM). Sepharose 121-130 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 14-37 20501194-8 1987 The IgG fraction was isolated from serum, and when coupled to cyanogen bromide activated Sepharose, could be used to purify tyrosine hydroxylase from crude extracts in a single step. Sepharose 89-98 tyrosine hydroxylase Rattus norvegicus 124-144 3025860-6 1987 The IgG-PTH-receptor complex was precipitated with staphylococcal protein A-Sepharose. Sepharose 76-85 parathyroid hormone Bos taurus 8-11 3025860-9 1987 The anti-PTH IgG was affinity purified by passage over a PTH-Sepharose column and used to make an immunoaffinity column. Sepharose 61-70 parathyroid hormone Rattus norvegicus 9-12 3446289-2 1987 When diclofenac sodium, was incorporated into the agarose gel at various concentrations below 100 micrograms/ml, it inhibited, in a dose-dependent fashion, spontaneous PMN migration and the directional migrations induced by both C5a-activated serum and peptide N-formyl-methionyl-leucyl-phenylalanine (FMLP). Sepharose 50-57 formyl peptide receptor 1 Homo sapiens 302-306 3540163-0 1987 Effectiveness of Sepharose-bound trypsin versus liquid-phase trypsin plus benzamidine for activation of inactive renin in human plasma. Sepharose 17-26 renin Homo sapiens 113-118 3625597-1 1987 We have purified the luteinizing hormone (LH)/human choriogonadotropin (hCG) receptor to homogeneity by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose. Sepharose 152-161 glycoprotein hormones, alpha polypeptide Homo sapiens 72-75 3625597-1 1987 We have purified the luteinizing hormone (LH)/human choriogonadotropin (hCG) receptor to homogeneity by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose. Sepharose 170-179 glycoprotein hormones, alpha polypeptide Homo sapiens 72-75 3625597-1 1987 We have purified the luteinizing hormone (LH)/human choriogonadotropin (hCG) receptor to homogeneity by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose. Sepharose 170-179 glycoprotein hormones, alpha polypeptide Homo sapiens 166-169 3820185-1 1987 Cathepsin B (EC 3.4.22.1) has been purified from rabbit testes to apparent homogeneity by chromatography on DE-52, affinity chromatography on organomercurial agarose and subsequent gel filtrations on Sephadex G-75. Sepharose 158-165 cathepsin B Oryctolagus cuniculus 0-11 2834593-2 1987 In the present study, phosphodiesterase, one of the typical calmodulin-dependent functional proteins, was purified from bovine lens by DEAE-cellulose chromatography, calmodulin-Sepharose 4B chromatography and Superose 12 chromatography. Sepharose 177-186 calmodulin Bos taurus 166-176 2444745-0 1987 [A modified method of determining antithrombin III activity by diffusion in thrombin agarose]. Sepharose 85-92 serpin family C member 1 Homo sapiens 34-50 2444745-0 1987 [A modified method of determining antithrombin III activity by diffusion in thrombin agarose]. Sepharose 85-92 coagulation factor II, thrombin Homo sapiens 38-46 3497327-4 1987 Interestingly, rosette-inhibiting factors could be removed by absorption with IgE-Sepharose from which they were subsequently eluated with acid buffer, indicating that the rosette inhibition was indeed mediated by IgE-BFs. Sepharose 82-91 immunoglobulin heavy constant epsilon Homo sapiens 78-81 3497327-4 1987 Interestingly, rosette-inhibiting factors could be removed by absorption with IgE-Sepharose from which they were subsequently eluated with acid buffer, indicating that the rosette inhibition was indeed mediated by IgE-BFs. Sepharose 82-91 immunoglobulin heavy constant epsilon Homo sapiens 214-217 2443861-5 1987 Step 3--additional purification of the AFP preparation on column Con-A-Sepharose. Sepharose 71-80 alpha fetoprotein Homo sapiens 39-42 3497849-1 1987 A fast-migrating precipitin peak which showed a reaction of partial immunochemical identity with the major von Willebrand factor (vWf) component was detected by crossed immunoelectrophoresis in agarose gel in plasma but not in platelets from a patient with type IIA von Willebrand"s disease (vWD). Sepharose 194-201 von Willebrand factor Homo sapiens 130-133 3030393-7 1986 The factor VIIIdes-797-1562 variant also bound to von Willebrand factor (vWF) immobilized on Sepharose. Sepharose 93-102 von Willebrand factor Homo sapiens 50-71 3030393-7 1986 The factor VIIIdes-797-1562 variant also bound to von Willebrand factor (vWF) immobilized on Sepharose. Sepharose 93-102 von Willebrand factor Homo sapiens 73-76 3098853-2 1986 In the assay a beta 2-microglobulin-beta-galactosidase conjugate competes with beta 2-microglobulin from the sample for the binding to anti-beta 2-microglobulin antibodies bound to small size agarose particles (micro-Sepharose) via a double antibody. Sepharose 192-199 beta-2-microglobulin Homo sapiens 15-35 2946691-8 1986 When the 16.8 S peak of bound [3H]vasopressin was further purified over a wheat germ lectin-Sepharose column, a GTPase activity co-eluted from the column with the protein-bound [3H]vasopressin. Sepharose 92-101 arginine vasopressin Rattus norvegicus 34-45 3100107-3 1986 This allowed measurement of apolipoproteins in lipidemic sera by SRID in agarose gel. Sepharose 73-80 apolipoprotein E Homo sapiens 28-43 3023356-1 1986 Effect of p-mercuribenzoate and organomercurial agarose gel on glucose-6-phosphatase of native and detergent-modified microsomes. Sepharose 48-55 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 63-84 3097137-4 1986 Furthermore, fractionation of CMS by protein A Sepharose chromatography demonstrated that the IgG fraction had an increased lethal activity compared with unfractionated serum; this result was not seen with VMS. Sepharose 47-56 resistance to Coccidioides immitis 1 Mus musculus 30-33 3470004-3 1986 The vinyl ketone inactivates rat ovarian 20 alpha-hydroxysteroid dehydrogenase, semi-purified by poly(L-lysine)-agarose column chromatography, in a rapid time-dependent manner. Sepharose 112-119 aldo-keto reductase family 1, member C3 Rattus norvegicus 41-78 3782796-3 1986 To confirm that cross-reactivities were due to a single antibody, immunoabsorption of a representative polyspecific monoclonal termed C11 (anti-DNA, anti-Sm) on either Sepharose-DNA or Sepharose-RIgG resulted in marked loss of activity to the three antigens DNA, Sm and RIgG compared with immunoabsorption on Sepharose-bovine serum albumin. Sepharose 168-177 polymerase (RNA) III (DNA directed) polypeptide K Mus musculus 134-137 3563966-4 1986 A normal affinity of antithrombin III for heparin was documented by heparin-sepharose chromatography. Sepharose 76-85 serpin family C member 1 Homo sapiens 21-37 3563966-5 1986 Affinity adsorption of the propositus" plasma to human alpha-thrombin immobilized on sepharose beads revealed defective binding of the antithrombin III to thrombin-sepharose. Sepharose 85-94 coagulation factor II, thrombin Homo sapiens 61-69 3563966-5 1986 Affinity adsorption of the propositus" plasma to human alpha-thrombin immobilized on sepharose beads revealed defective binding of the antithrombin III to thrombin-sepharose. Sepharose 164-173 coagulation factor II, thrombin Homo sapiens 61-69 3563966-5 1986 Affinity adsorption of the propositus" plasma to human alpha-thrombin immobilized on sepharose beads revealed defective binding of the antithrombin III to thrombin-sepharose. Sepharose 164-173 serpin family C member 1 Homo sapiens 135-151 3023339-2 1986 The receptor for nerve growth factor (NGF) was purified from Triton X-100 extracts of sympathetic ganglia membranes by affinity chromatography on NGF-Sepharose. Sepharose 150-159 beta-nerve growth factor Oryctolagus cuniculus 17-36 3023339-2 1986 The receptor for nerve growth factor (NGF) was purified from Triton X-100 extracts of sympathetic ganglia membranes by affinity chromatography on NGF-Sepharose. Sepharose 150-159 beta-nerve growth factor Oryctolagus cuniculus 38-41 3023339-2 1986 The receptor for nerve growth factor (NGF) was purified from Triton X-100 extracts of sympathetic ganglia membranes by affinity chromatography on NGF-Sepharose. Sepharose 150-159 beta-nerve growth factor Oryctolagus cuniculus 146-149 3782111-2 1986 We have purified the luteinizing hormone (LH)/human choriogonadotropin (hCG) receptor by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose. Sepharose 137-146 glycoprotein hormones, alpha polypeptide Homo sapiens 72-75 3782111-2 1986 We have purified the luteinizing hormone (LH)/human choriogonadotropin (hCG) receptor by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose. Sepharose 155-164 glycoprotein hormones, alpha polypeptide Homo sapiens 72-75 3782111-2 1986 We have purified the luteinizing hormone (LH)/human choriogonadotropin (hCG) receptor by sequential affinity column on wheat germ lectin-Sepharose and hCG-Sepharose. Sepharose 155-164 glycoprotein hormones, alpha polypeptide Homo sapiens 151-154 3814093-2 1986 Cathepsin S was purified from bovine spleen by acid autolysis, (NH4)2SO4 fractionation and chromatography on CM-Sephadex C-50, CM-cellulose and activated-thiol-Sepharose. Sepharose 160-169 cathepsin S Bos taurus 0-11 3814356-3 1986 Purified M73/PK1 was obtained by ammonium sulfate precipitation and Protein A-agarose affinity chromatography and was coupled to CNBr-activated Sepharose 6MB. Sepharose 78-85 prokineticin 1 Bos taurus 13-16 3814356-3 1986 Purified M73/PK1 was obtained by ammonium sulfate precipitation and Protein A-agarose affinity chromatography and was coupled to CNBr-activated Sepharose 6MB. Sepharose 144-153 prokineticin 1 Bos taurus 13-16 2430690-3 1986 A composite sodium dodecyl sulfate: polyacrylamide:1.0% agarose gel resolved the CA 125 activity from the three sources of antigen into disperse bands of similar electrophoretic mobilities with apparent masses of 200,000 to 1 million daltons. Sepharose 56-63 mucin 16, cell surface associated Homo sapiens 81-87 3552334-4 1986 MIF was assayed with a recently developed test system using the human monocytoid cell-line U937 as indicator cells in agarose microdroplets. Sepharose 118-125 macrophage migration inhibitory factor Homo sapiens 0-3 3104046-5 1986 The calcium-binding ability of CaBP was demonstrated by increased electrophoretic mobility of CaBP in the presence of EDTA as demonstrated by immunoblotting of agarose gels. Sepharose 160-167 centrin 1 Homo sapiens 31-35 3104046-5 1986 The calcium-binding ability of CaBP was demonstrated by increased electrophoretic mobility of CaBP in the presence of EDTA as demonstrated by immunoblotting of agarose gels. Sepharose 160-167 centrin 1 Homo sapiens 94-98 3026891-3 1986 All in vivo and in vitro uvsY transcripts show anomalous migration in agarose gels. Sepharose 70-77 recombination mediator protein UvsY Escherichia phage T4 25-29 3546494-8 1986 Affinity chromatography with H.77 Sepharose was used to purify active renin from anephric and normal plasma following treatment with trypsin. Sepharose 34-43 renin Homo sapiens 70-75 3023795-2 1986 Both antisera were adsorbed against human C-peptide conjugated to Sepharose, following which cross-reactivity to insulin and C-peptide was less than 0.001%. Sepharose 66-75 insulin Homo sapiens 42-51 3023795-2 1986 Both antisera were adsorbed against human C-peptide conjugated to Sepharose, following which cross-reactivity to insulin and C-peptide was less than 0.001%. Sepharose 66-75 insulin Homo sapiens 113-120 3023795-2 1986 Both antisera were adsorbed against human C-peptide conjugated to Sepharose, following which cross-reactivity to insulin and C-peptide was less than 0.001%. Sepharose 66-75 insulin Homo sapiens 125-134 3112509-4 1986 We purified the bottom-less receptor to homogeneity by affinity chromatography using an IL-2-Sepharose column. Sepharose 93-102 interleukin 2 Homo sapiens 88-92 3112509-5 1986 The affinity (Kd value) of the 125I-labeled bottom-less receptor to IL-2 coupled to Sepharose beads was estimated to be 4.5 microM. Sepharose 84-93 interleukin 2 Homo sapiens 68-72 2948272-0 1986 Phagocytosis of agarose beads by receptors for C3b (CR1) and iC3b (CR3) on human alveolar macrophages cultured on fibronectin in vitro. Sepharose 16-23 complement C3 Homo sapiens 47-50 2948272-0 1986 Phagocytosis of agarose beads by receptors for C3b (CR1) and iC3b (CR3) on human alveolar macrophages cultured on fibronectin in vitro. Sepharose 16-23 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 52-55 2948272-0 1986 Phagocytosis of agarose beads by receptors for C3b (CR1) and iC3b (CR3) on human alveolar macrophages cultured on fibronectin in vitro. Sepharose 16-23 teratocarcinoma-derived growth factor 1 pseudogene 3 Homo sapiens 67-70 2948272-0 1986 Phagocytosis of agarose beads by receptors for C3b (CR1) and iC3b (CR3) on human alveolar macrophages cultured on fibronectin in vitro. Sepharose 16-23 fibronectin 1 Homo sapiens 114-125 3780967-1 1986 The acute anti-lipolytic effect of human growth hormone (hGH) in maximally noradrenaline-stimulated intact rat adipocytes was selectively associated with increased phosphorylation of a 46 kDa plasma membrane protein which was highly enriched by hGH-Sepharose chromatography. Sepharose 249-258 growth hormone 1 Homo sapiens 41-55 2948561-1 1986 P-57 is a neurospecific calmodulin binding protein that was discovered by virtue of its unusual interactions with calmodulin-Sepharose [Andreasen, T. J., Luetje, C. W., Heideman, W., & Storm, D. R. (1983) Biochemistry 22, 4615-4618; Cimler, B. M., Andreasen, T. J., Andreasen, K. I., & Storm, D. R. (1985) J. Biol. Sepharose 125-134 calmodulin Bos taurus 24-34 2948561-1 1986 P-57 is a neurospecific calmodulin binding protein that was discovered by virtue of its unusual interactions with calmodulin-Sepharose [Andreasen, T. J., Luetje, C. W., Heideman, W., & Storm, D. R. (1983) Biochemistry 22, 4615-4618; Cimler, B. M., Andreasen, T. J., Andreasen, K. I., & Storm, D. R. (1985) J. Biol. Sepharose 125-134 calmodulin Bos taurus 114-124 2948561-4 1986 In contrast to other calmodulin binding proteins, P-57 has higher affinity for calmodulin-Sepharose in the absence of calcium compared to that in the presence of calcium. Sepharose 90-99 calmodulin Bos taurus 79-89 3023087-1 1986 Pig plasma gelsolin has been prepared by a revised method involving poly(ethylene glycol) precipitation, chromatography on CM-cellulose and affinity chromatography on actin-Sepharose. Sepharose 173-182 GSN Sus scrofa 11-19 3023087-2 1986 Pig platelet gelsolin has been prepared by chromatography on DEAE-cellulose and actin-Sepharose. Sepharose 86-95 GSN Sus scrofa 13-21 3023089-14 1986 Pig plasma gelsolin is heterogeneous on isoelectric focussing gels in urea, but the two types of gelsolin separated on actin-Sepharose do not correspond to specific isoelectric species. Sepharose 125-134 GSN Sus scrofa 97-105 3805203-3 1986 The blue Sepharose column separates HMG I and Y completely from HMG 14 and 17, and should therefore be an useful tool for the identification of these proteins which in several reports have been confused with HMG 14 and 17. Sepharose 9-18 high mobility group nucleosome binding domain 1 Homo sapiens 64-70 3805203-3 1986 The blue Sepharose column separates HMG I and Y completely from HMG 14 and 17, and should therefore be an useful tool for the identification of these proteins which in several reports have been confused with HMG 14 and 17. Sepharose 9-18 high mobility group nucleosome binding domain 1 Homo sapiens 208-214 3805203-4 1986 HMG I and Y on the one hand and HMG 14 and 17 on the other exhibited considerable differences in their affinities for Blue Sepharose, probably reflecting fundamental differences in biological function. Sepharose 123-132 high mobility group nucleosome binding domain 1 Homo sapiens 32-38 2881538-5 1986 The effect of insulin on acetyl-CoA carboxylase activity, but not the effect on phosphorylation, was lost on purification of the enzyme on avidin-Sepharose. Sepharose 146-155 insulin Homo sapiens 14-21 3492941-3 1986 Mutant adenosine deaminase has been isolated from heterozygous and homozygous deficient lymphoblast cell lines with the aid of an affinity matrix consisting of coformycin (a potent inhibitor of the enzyme) as the affinity ligand, bound to 3,3"-iminobispropylamine-derivatized Sepharose. Sepharose 276-285 adenosine deaminase Homo sapiens 7-26 3771562-4 1986 Prothrombin Barcelona and prothrombin were isolated from patient plasma and normal plasma, respectively, in a single-step, high-yield immunoaffinity purification using conformation-specific antibodies immobilized on Sepharose. Sepharose 216-225 coagulation factor II, thrombin Homo sapiens 0-11 3094583-2 1986 The method utilizes the difference between the rate of electrophoretic migration of apolipoprotein A-I associated with HDL (alpha) and "free" apolipoprotein A-I (pre-beta) in agarose gel. Sepharose 175-182 apolipoprotein A1 Homo sapiens 84-102 3094583-2 1986 The method utilizes the difference between the rate of electrophoretic migration of apolipoprotein A-I associated with HDL (alpha) and "free" apolipoprotein A-I (pre-beta) in agarose gel. Sepharose 175-182 apolipoprotein A1 Homo sapiens 142-160 3490474-4 1986 The PAI of one of the two melanoma cell (MJZJ)-conditioned media found to contain PAI activity was purified to apparent homogeneity employing concanavalin A-Sepharose chromatography, gel filtration on Sephadex G-150, chromatography on Affi-Gel blue, and affinity chromatography on a Sepharose 4B immobilized monoclonal anti-t-PA IgG column. Sepharose 283-295 serpin family E member 1 Homo sapiens 4-7 3099625-5 1986 Radiolabeled factor VIII antibody: factor VIII protein immune complexes were bound to agarose beads by mouse monoclonal antifactor VIII R:Ag antibody. Sepharose 86-93 cytochrome c oxidase subunit 8A Homo sapiens 20-24 3099625-5 1986 Radiolabeled factor VIII antibody: factor VIII protein immune complexes were bound to agarose beads by mouse monoclonal antifactor VIII R:Ag antibody. Sepharose 86-93 cytochrome c oxidase subunit 8A Homo sapiens 42-46 3099625-5 1986 Radiolabeled factor VIII antibody: factor VIII protein immune complexes were bound to agarose beads by mouse monoclonal antifactor VIII R:Ag antibody. Sepharose 86-93 cytochrome c oxidase subunit 8A Homo sapiens 42-46 2949690-1 1986 In addition to fibronectin-derived peptides, several immunologically distinct peptides have been found to co-purify with fibronectin on gelatin-sepharose affinity chromatography of bovine plasma. Sepharose 144-153 fibronectin 1 Bos taurus 121-132 2432915-9 1986 AFP mRNA from nude mouse tumours migrated as a 20S discrete band on agarose gel electrophoresis, whereas a more complex hybridization pattern was evident in human tumours. Sepharose 68-75 alpha fetoprotein Mus musculus 0-3 2430736-1 1986 Affinity chromatography of serum on lentil lectin bound to Sepharose 4B has been used to identify different forms of alpha-fetoprotein in serum of patients with liver disease. Sepharose 59-68 alpha fetoprotein Homo sapiens 117-134 3096594-1 1986 In this method for clinical measurement of beta 2-microglobulin, a 10 g/L agarose gel containing anti-beta 2-microglobulin is used, with subsequent staining with Coomassie Blue. Sepharose 74-81 beta-2-microglobulin Homo sapiens 43-63 3096594-1 1986 In this method for clinical measurement of beta 2-microglobulin, a 10 g/L agarose gel containing anti-beta 2-microglobulin is used, with subsequent staining with Coomassie Blue. Sepharose 74-81 beta-2-microglobulin Homo sapiens 102-122 3024962-2 1986 The hybrid polypeptide was recognized by an anti-(human plasma fibronectin) serum and bound specifically to gelatin-Sepharose. Sepharose 116-125 fibronectin 1 Homo sapiens 63-74 3758208-3 1986 The suppression of contraction observed when fibronectin was eliminated from serum, either by affinity chromatography on gelatin-agarose columns or by precipitation with anti-fibronectin antibodies, showed that fibronectin is critical for the contraction. Sepharose 129-136 fibronectin 1 Homo sapiens 45-56 3098666-5 1986 Cross-immunoelectrophoresis of fulminant hepatic failure plasma for fibronectin on agarose plates gave an additional slower migrating peak in 15 of the 29 patients, as well as that of fibronectin, which corresponded to the fibronectin complex reported by other workers in leukemia. Sepharose 83-90 fibronectin 1 Homo sapiens 68-79 3557273-0 1986 [Purification and characterization of lathosterol 5-desaturase from rat liver microsomes by cytochrome b5-sepharose affinity column chromatography: evidence for the non-heme iron protein]. Sepharose 106-115 sterol-C5-desaturase Rattus norvegicus 38-62 3557273-0 1986 [Purification and characterization of lathosterol 5-desaturase from rat liver microsomes by cytochrome b5-sepharose affinity column chromatography: evidence for the non-heme iron protein]. Sepharose 106-115 cytochrome b5 type A Rattus norvegicus 92-105 3557273-1 1986 Lathosterol 5-desaturase, which catalyzes introduction of a delta 5 bond into lathosterol to form 7-dehydrocholesterol, was purified up to 2000-4000-fold with a 13-18% yield from rat liver microsomes by cytochrome b5-Sepharose affinity chromatography followed by isoelectric focusing. Sepharose 217-226 sterol-C5-desaturase Rattus norvegicus 0-24 3781560-1 1986 A total of 1242 individuals from six Chinese ethnic groups were studied with respect to the glyoxalase I polymorphism using agarose gel electrophoresis. Sepharose 124-131 glyoxalase I Homo sapiens 92-104 3825816-3 1986 AR was purified from 1,700 g of benign prostatic hypertrophic tissue by combining affinity chromatography of heparin Sepharose CL-6B and of 17 alpha-carboxy-hexamethyl-17-hydroxy-4-androstane-3-one Sepharose 4B. Sepharose 117-126 androgen receptor Homo sapiens 0-2 3825818-3 1986 With 855 g of benign prostatic hypertrophic tissue as starting material, the androgen receptor (AR) was purified by combining the affinity chromatography of heparin Sepharose CL-6B and of R1881-carboxymethyloxime-albumin Sepharose 4B. Sepharose 165-174 androgen receptor Homo sapiens 96-98 2434472-8 1986 About 12-20% of the vitronectin molecules in plasma were found to bind to heparin-Sepharose under physiological conditions. Sepharose 82-91 vitronectin Homo sapiens 20-31 2950080-2 1986 The solubilized CR1 was purified using DEAE-Sephacel, C3-Sepharose, and anti-CR1-Sepharose column chromatographies. Sepharose 57-66 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 16-19 2950080-2 1986 The solubilized CR1 was purified using DEAE-Sephacel, C3-Sepharose, and anti-CR1-Sepharose column chromatographies. Sepharose 81-90 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 16-19 2950080-2 1986 The solubilized CR1 was purified using DEAE-Sephacel, C3-Sepharose, and anti-CR1-Sepharose column chromatographies. Sepharose 81-90 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 77-80 3491089-3 1986 Western blot analysis and retention experiments with protein A-Sepharose indicate that the antibodies are specific for interleukin-2. Sepharose 63-72 interleukin 2 Homo sapiens 119-132 3760117-3 1986 Agarose gel electrophoresis of poly(A) RNA extracts of adenoma tissues followed by hybridization with v-myc DNA yielded two distinct c-myc mRNA species [2.1 and 4.0 kilobases (kb)]. Sepharose 0-7 MYC proto-oncogene, bHLH transcription factor Homo sapiens 133-138 3098853-2 1986 In the assay a beta 2-microglobulin-beta-galactosidase conjugate competes with beta 2-microglobulin from the sample for the binding to anti-beta 2-microglobulin antibodies bound to small size agarose particles (micro-Sepharose) via a double antibody. Sepharose 192-199 galactosidase beta 1 Homo sapiens 36-54 3098853-2 1986 In the assay a beta 2-microglobulin-beta-galactosidase conjugate competes with beta 2-microglobulin from the sample for the binding to anti-beta 2-microglobulin antibodies bound to small size agarose particles (micro-Sepharose) via a double antibody. Sepharose 192-199 beta-2-microglobulin Homo sapiens 79-99 3818580-1 1986 Human platelet membrane glycoproteins IIb (GPIIb) and IIIa (GPIIIa), which have been proposed to be subunits of a receptor for fibrinogen, were purified from Triton X-100-solubilized platelet membranes by affinity chromatography on a concanavalin A (Con A)-Sepharose column followed by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 257-266 integrin subunit beta 3 Homo sapiens 60-66 3098853-2 1986 In the assay a beta 2-microglobulin-beta-galactosidase conjugate competes with beta 2-microglobulin from the sample for the binding to anti-beta 2-microglobulin antibodies bound to small size agarose particles (micro-Sepharose) via a double antibody. Sepharose 192-199 beta-2-microglobulin Homo sapiens 79-99 3098853-2 1986 In the assay a beta 2-microglobulin-beta-galactosidase conjugate competes with beta 2-microglobulin from the sample for the binding to anti-beta 2-microglobulin antibodies bound to small size agarose particles (micro-Sepharose) via a double antibody. Sepharose 217-226 beta-2-microglobulin Homo sapiens 15-35 3098853-2 1986 In the assay a beta 2-microglobulin-beta-galactosidase conjugate competes with beta 2-microglobulin from the sample for the binding to anti-beta 2-microglobulin antibodies bound to small size agarose particles (micro-Sepharose) via a double antibody. Sepharose 217-226 galactosidase beta 1 Homo sapiens 36-54 3098853-2 1986 In the assay a beta 2-microglobulin-beta-galactosidase conjugate competes with beta 2-microglobulin from the sample for the binding to anti-beta 2-microglobulin antibodies bound to small size agarose particles (micro-Sepharose) via a double antibody. Sepharose 217-226 beta-2-microglobulin Homo sapiens 79-99 3098853-2 1986 In the assay a beta 2-microglobulin-beta-galactosidase conjugate competes with beta 2-microglobulin from the sample for the binding to anti-beta 2-microglobulin antibodies bound to small size agarose particles (micro-Sepharose) via a double antibody. Sepharose 217-226 beta-2-microglobulin Homo sapiens 79-99 3787189-2 1986 We also found that monoclonal antibodies against human C3c, C3g, and a C9-neoantigen as well as polyclonal antibodies against human C5 and C9, bound to agarose beads that had been kept with the macrophage cultures. Sepharose 152-159 Rap guanine nucleotide exchange factor 1 Homo sapiens 60-63 3787189-2 1986 We also found that monoclonal antibodies against human C3c, C3g, and a C9-neoantigen as well as polyclonal antibodies against human C5 and C9, bound to agarose beads that had been kept with the macrophage cultures. Sepharose 152-159 complement C5 Homo sapiens 132-141 3811292-1 1986 Interaction of native and thrombin-modified human low density lipoproteins (LDL) with immobilized homologous fibronectin (either covalently bound to Sepharose or adsorbed from blood serum on collagen-Sepharose) was studied. Sepharose 149-158 fibronectin 1 Homo sapiens 109-120 3489778-12 1986 Interestingly, it was also found that B151-TRF2 activity was trapped and eluted from GlcNAc-coupled agarose beads. Sepharose 100-107 telomeric repeat binding factor 2 Mus musculus 43-47 3811292-3 1986 Chromatography of native and thrombinmodified LDL on fibronectin-Sepharose showed that 30% of the modified LDL and 2% of native LDL were bound to fibronectin-Sepharose at physiological pH values and NaCl concentrations. Sepharose 65-74 fibronectin 1 Homo sapiens 53-64 3811292-3 1986 Chromatography of native and thrombinmodified LDL on fibronectin-Sepharose showed that 30% of the modified LDL and 2% of native LDL were bound to fibronectin-Sepharose at physiological pH values and NaCl concentrations. Sepharose 158-167 fibronectin 1 Homo sapiens 146-157 3811292-4 1986 Study of the interaction of LDL with fibronectin adsorbed on collagen-Sepharose showed that thrombin-treated LDL partially released fibronectin from the sorbent due to the formation of a modified LDL-fibronectin complex. Sepharose 70-79 coagulation factor II, thrombin Homo sapiens 92-100 3811292-4 1986 Study of the interaction of LDL with fibronectin adsorbed on collagen-Sepharose showed that thrombin-treated LDL partially released fibronectin from the sorbent due to the formation of a modified LDL-fibronectin complex. Sepharose 70-79 fibronectin 1 Homo sapiens 37-48 3101222-1 1986 A single-chain urokinase-type plasminogen activator (A431sc-uPA) was purified approximately 18,000-fold from A431 human epidermoid carcinoma cell supernatants by monoclonal antibody immunoaffinity chromatography on 5B4-agarose and ion-exchange FPLC (overall yield 63%). Sepharose 219-226 plasminogen activator, urokinase Homo sapiens 15-51 3021732-4 1986 Human recombinant t-PA deletion-mutant proteins were prepared and their ability to bind to lysine-Sepharose was investigated. Sepharose 98-107 plasminogen activator, tissue type Homo sapiens 18-22 2433787-4 1986 The antibody has an affinity constant for uPA-Sepharose of 1.42 X 10(7) M-1, calculated from equilibrium binding data, and can be used for one step purification of HMW-uPA by immunoaffinity chromatography. Sepharose 46-55 plasminogen activator, urokinase Homo sapiens 42-45 2433787-4 1986 The antibody has an affinity constant for uPA-Sepharose of 1.42 X 10(7) M-1, calculated from equilibrium binding data, and can be used for one step purification of HMW-uPA by immunoaffinity chromatography. Sepharose 46-55 cilia and flagella associated protein 97 Homo sapiens 164-167 2433787-4 1986 The antibody has an affinity constant for uPA-Sepharose of 1.42 X 10(7) M-1, calculated from equilibrium binding data, and can be used for one step purification of HMW-uPA by immunoaffinity chromatography. Sepharose 46-55 plasminogen activator, urokinase Homo sapiens 168-171 3769936-1 1986 Two forms of acidic fibroblast growth factor were isolated from bovine brain by a combination of ammonium sulfate precipitation, cation-exchange chromatography, heparin-Sepharose affinity chromatography, and reverse-phase high-performance liquid chromatography. Sepharose 169-178 fibroblast growth factor 1 Bos taurus 13-44 2878123-9 1986 Anti-CD3 monoclonal antibodies coupled to agarose, but not anti-T200-agarose, induced the release of high levels of LT and IL-2. Sepharose 42-49 interleukin 2 Homo sapiens 123-127 3114734-7 1987 The separation appears to result from a combination of hydrophobic and electrostatic interactions of chymosin with Blue agarose. Sepharose 120-127 chymosin Bos taurus 101-109 3810553-2 1986 Using canine AT III purified by heparin-Sepharose affinity chromatography, antiserum against canine AT III was raised in rabbit. Sepharose 40-49 serpin family C member 1 Canis lupus familiaris 100-106 3020033-3 1986 Both the nonoccupied and in vitro occupied forms of the receptors interacted with heparin-Sepharose but with varying strength, as determined by ligand binding assays or an enzyme-linked immunosorbent assay based on a monoclonal antibody against the steroid- and DNA-binding Mr approximately 94,000 glucocorticoid receptor protein. Sepharose 90-99 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 298-321 3531211-3 1986 The antibody was coupled to Sepharose and used as an independent method for purifying active TGF alpha. Sepharose 28-37 transforming growth factor alpha Homo sapiens 93-102 3759265-2 1986 Independent, HRAS1-selected chromosome-mediated transformants displayed distinctive cellular morphologies in monolayer culture and colony-forming abilities in low-melting-point agarose. Sepharose 177-184 Harvey rat sarcoma virus oncogene Mus musculus 13-18 3093793-0 1986 Rapid and high-yield purification of porcine heart tissue-type plasminogen activator by heparin-sepharose choromatography. Sepharose 96-105 plasminogen activator, tissue type Homo sapiens 51-84 3790255-1 1986 The malate dehydrogenase activity in the cytosolic fraction isolated from chicken hepatocytes is resolved by DEAE-Sephacel chromatography in three active, electrophoretically distinct, species obtained in homogeneous form by affinity chromatography on 5"-AMP-Sepharose and Blue-Sepharose. Sepharose 259-268 malic enzyme 1 Gallus gallus 4-24 3768512-3 1986 Neutrophils were brought in contact with purified homologous fibronectin previously bound to gelatin-Sepharose granules. Sepharose 101-110 fibronectin 1 Homo sapiens 61-72 2428547-0 1986 Microheterogeneity of human transferrin as revealed by agarose gel electrophoresis with an iron-specific stain. Sepharose 55-62 transferrin Homo sapiens 28-39 2428599-2 1986 Progesterone receptor purified from oviduct cytosol by chromatography on deoxycorticosterone-Sepharose and heparin-agarose was used as the immunizing antigen. Sepharose 93-102 progesterone receptor Homo sapiens 0-21 3743668-1 1986 Antibodies specific for chromosomal protein HMG-17 were immobilized on CNBr-Sepharose and the resulting immunoaffinity column was used to purify chromatin segments enriched in HMG-17. Sepharose 76-85 high mobility group nucleosomal binding domain 2 Homo sapiens 44-50 3770805-4 1986 Absorption of tolerogenic serum with anti-OVA antibody coupled to Sepharose beads effectively removed the tolerogenic moiety from the serum and confirmed that not only was tolerogenicity associated with the presence of antigen, but that binding sites for antibody were intact on this tolerogenic form of OVA. Sepharose 66-75 serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene Mus musculus 42-45 3818560-1 1986 Human platelet membrane glycoprotein IV (GPIV), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of Lens culinaris agglutinin-Sepharose and wheat germ agglutinin-Sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). Sepharose 183-192 CD36 molecule Homo sapiens 24-39 3818560-1 1986 Human platelet membrane glycoprotein IV (GPIV), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of Lens culinaris agglutinin-Sepharose and wheat germ agglutinin-Sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). Sepharose 183-192 CD36 molecule Homo sapiens 41-45 3818560-1 1986 Human platelet membrane glycoprotein IV (GPIV), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of Lens culinaris agglutinin-Sepharose and wheat germ agglutinin-Sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). Sepharose 219-228 CD36 molecule Homo sapiens 24-39 3020090-6 1986 The MCF/IL-1 inhibitor(s), which also acts on human recombinant IL-1 beta, is approximately 25-35 kD, is not retained on concanavalin A-Sepharose column and can be partially destroyed with urea and boiling. Sepharose 136-145 interleukin 1 receptor antagonist Homo sapiens 8-22 3023523-1 1986 A rapid, reliable method for the simultaneous separation of adenosine deaminase, adenylate kinase, and carbonic anhydrase II by agarose gel electrophoresis is presented. Sepharose 128-135 adenosine deaminase Homo sapiens 60-79 3023523-1 1986 A rapid, reliable method for the simultaneous separation of adenosine deaminase, adenylate kinase, and carbonic anhydrase II by agarose gel electrophoresis is presented. Sepharose 128-135 carbonic anhydrase 2 Homo sapiens 103-124 3099175-2 1986 It is known that SAP binds Sepharose in the presence of calcium. Sepharose 27-36 amyloid P component, serum Homo sapiens 17-20 3099175-3 1986 When purified 125I-C1q was incubated with SAP prior to Sepharose affinity chromatography, 125I-C1q was retained. Sepharose 55-64 complement C1q A chain Homo sapiens 19-22 3017990-4 1986 A murine IgG1 mAb specific for the high affinity p72 Fc receptor (designated mAb FcR32 or simply mAb 32) bound to the same p72 precipitated by Sepharose-human IgG as shown by preclearing experiments and by identical isoelectric focussing patterns. Sepharose 143-152 DEAD-box helicase 17 Homo sapiens 49-52 3017990-4 1986 A murine IgG1 mAb specific for the high affinity p72 Fc receptor (designated mAb FcR32 or simply mAb 32) bound to the same p72 precipitated by Sepharose-human IgG as shown by preclearing experiments and by identical isoelectric focussing patterns. Sepharose 143-152 DEAD-box helicase 17 Homo sapiens 123-126 3745204-3 1986 Purified human fibrinogen specifically bound to HA-Sepharose to a greater extent (greater than 5-fold) than did alpha 1-acid glycoprotein, DNaseI, ovalbumin, haptoglobin, or lysozyme. Sepharose 51-60 fibrinogen beta chain Homo sapiens 15-25 3745204-5 1986 Treatment of HA-Sepharose containing bound 125I-fibrinogen with ovine testicular hyaluronidase released 44% of the 125I radioactivity, indicating that fibrinogen was specifically bound to HA. Sepharose 16-25 fibrinogen beta chain Homo sapiens 48-58 3745204-5 1986 Treatment of HA-Sepharose containing bound 125I-fibrinogen with ovine testicular hyaluronidase released 44% of the 125I radioactivity, indicating that fibrinogen was specifically bound to HA. Sepharose 16-25 fibrinogen beta chain Homo sapiens 151-161 3745204-6 1986 Moreover, 125I-fibrinogen bound to HA-Sepharose could be displaced by free HA but not by either of the monosaccharide components of this polymer, glucuronic acid, or N-acetylglucosamine. Sepharose 38-47 fibrinogen beta chain Homo sapiens 15-25 3745204-10 1986 Small HA oligosaccharides (Mr = 3900) were only 50% as effective as larger HA (Mr = 8 X 10(5)) in eluting bound 125I-fibrinogen from HA-Sepharose. Sepharose 136-145 fibrinogen beta chain Homo sapiens 117-127 3745204-12 1986 Additionally, the amount of 125I-fibrinogen bound to HA-Sepharose was directly related to the size of the HA-amine linked to the affinity support. Sepharose 56-65 fibrinogen beta chain Homo sapiens 33-43 3091600-1 1986 5"-Methylthioadenosine phosphorylase has been purified to homogeneity (30,000-fold) from human full-term placenta by a procedure involving covalent chromatography on organomercurial-agarose as the major step. Sepharose 182-189 methylthioadenosine phosphorylase Homo sapiens 0-36 3527274-1 1986 Inactive renin was purified to apparent homogeneity from human plasma by ion exchange, gel filtration, Affi-Gel blue, immunoaffinity chromatography on profragment-specific IgG coupled to Sepharose, and preparative HPLC. Sepharose 187-196 renin Homo sapiens 9-14 3756191-8 1986 First, post-heparin plasma lipoprotein lipase activity and immunoreactivity detected by an enzyme-linked immunosorbent assay (ELISA) co-eluted during heparin-agarose and phenyl-Sepharose chromatography. Sepharose 158-165 lipoprotein lipase Homo sapiens 27-45 3756191-8 1986 First, post-heparin plasma lipoprotein lipase activity and immunoreactivity detected by an enzyme-linked immunosorbent assay (ELISA) co-eluted during heparin-agarose and phenyl-Sepharose chromatography. Sepharose 176-186 lipoprotein lipase Homo sapiens 27-45 3741883-2 1986 The resulting cortexolone-Sepharose absorbed easily the cytosolic chick thymus glucocorticoid receptor. Sepharose 26-35 nuclear receptor subfamily 3 group C member 1 Gallus gallus 79-102 3767285-0 1986 A method for the identification of apolipoprotein E isoforms employing chemical precipitation and flat bed isoelectric focusing in agarose. Sepharose 131-138 apolipoprotein E Homo sapiens 35-51 3777429-0 1986 Purification of human serum vitamin D-binding protein by 25-hydroxyvitamin D3-Sepharose chromatography. Sepharose 78-87 GC vitamin D binding protein Homo sapiens 28-53 3541913-1 1986 An actin-like protein was purified to apparent homogeneity from chick-embryo homogenates and chick-embryo fibroblasts by the use of poly-L-proline-agarose affinity chromatography; we therefore refer to this protein as PBP (poly-L-proline-binding protein). Sepharose 147-154 actin, beta Gallus gallus 3-8 3541913-2 1986 PBP binds to deoxyribonuclease-agarose, co-migrates with known actin standards on SDS/polyacrylamide-gel electrophoresis, and has an amino acid composition similar to that of actin. Sepharose 31-38 phosphatidylethanolamine binding protein 1 Gallus gallus 0-3 2430744-3 1986 When TPO was incubated with IgG from sera followed by treatment with protein A-Sepharose and centrifugation, the remaining TPO activities in the supernatant fraction were lower in most of the patients, as compared to normal controls. Sepharose 79-88 thyroid peroxidase Homo sapiens 5-8 3019243-5 1986 Binding to concanavalin A-Sepharose suggested that the TNF receptor is a glycoprotein. Sepharose 26-35 tumor necrosis factor Homo sapiens 55-58 2946669-3 1986 When the ATPase fraction was applied to a Sepharose 4B column, 90% of the ATPase activity was recovered in a spectrin, actin, and band 4.1 complex fraction and none was detected in the spectrin fraction. Sepharose 42-51 dynein axonemal heavy chain 8 Homo sapiens 9-15 3530784-7 1986 The binding of p150,95 and CR3 to iC3b-Sepharose is specific as neither binds to C3u-Sepharose. Sepharose 39-48 chromatin assembly factor 1 subunit A Homo sapiens 15-19 2946669-3 1986 When the ATPase fraction was applied to a Sepharose 4B column, 90% of the ATPase activity was recovered in a spectrin, actin, and band 4.1 complex fraction and none was detected in the spectrin fraction. Sepharose 42-51 dynein axonemal heavy chain 8 Homo sapiens 74-80 2946669-3 1986 When the ATPase fraction was applied to a Sepharose 4B column, 90% of the ATPase activity was recovered in a spectrin, actin, and band 4.1 complex fraction and none was detected in the spectrin fraction. Sepharose 42-51 erythrocyte membrane protein band 4.1 Homo sapiens 130-138 2427570-8 1986 To induce IL 2 synthesis, the necessity to cross-link anti-CD2 Ab was demonstrated by coupling one Ab on Sepharose beads and adding the second Ab in the soluble phase: under these circumstances, anti-CD2 pairs were mitogenic solely in the presence of AC. Sepharose 105-114 CD2 molecule Homo sapiens 59-62 2427570-8 1986 To induce IL 2 synthesis, the necessity to cross-link anti-CD2 Ab was demonstrated by coupling one Ab on Sepharose beads and adding the second Ab in the soluble phase: under these circumstances, anti-CD2 pairs were mitogenic solely in the presence of AC. Sepharose 105-114 CD2 molecule Homo sapiens 200-203 3489033-5 1986 Activation with anti-receptor antibody conjugated to Sepharose 4B beads could be demonstrated in the presence of some exogenous cofactors, such as IL 2 and PMA, but not in the presence of recombinant IL 1. Sepharose 53-65 interleukin 2 Mus musculus 147-151 3528287-4 1986 In a single cell agarose assay, MC of Mac-1-deficient patients formed fewer effector-target cell conjugates in the presence of specific anti-HSV antibody. Sepharose 17-24 integrin subunit alpha M Homo sapiens 38-43 3025822-8 1986 Soluble covalently labeled VIP receptors from guinea pig and human lung bound to and were specifically eluted from agarose-linked wheat germ agglutinin columns. Sepharose 115-122 VIP peptides Cavia porcellus 27-30 3488995-7 1986 When C3 was cleaved with trypsin, C3b and C3d but not C3c bound to laminin-Sepharose. Sepharose 75-84 complement C3 Homo sapiens 34-37 3488995-7 1986 When C3 was cleaved with trypsin, C3b and C3d but not C3c bound to laminin-Sepharose. Sepharose 75-84 endogenous retrovirus group K member 13 Homo sapiens 42-45 3461485-3 1986 These findings have been exploited to highly purify TCSG from mouse L (H-2k) cells in one step by Iak antigen Sepharose affinity chromatography. Sepharose 110-119 aurora kinase A Mus musculus 98-101 3529086-2 1986 HGF was purified from rat platelets to homogeneity by a three-step procedure: stimulation of its release from platelets by thrombin, cation-exchanger fast protein liquid chromatography on a Mono S column, and heparin-Sepharose chromatography. Sepharose 217-226 hepatocyte growth factor Rattus norvegicus 0-3 2426267-1 1986 A protein preparation that specifically binds insulin-like growth factors (IGFs) I and II was purified from medium conditioned by rat liver BRL-3A cells using molecular sieve chromatography in 1 M acetic acid followed by affinity chromatography on IGF-II-agarose. Sepharose 255-262 insulin-like growth factor 1 Rattus norvegicus 46-89 3015966-2 1986 Insulin-like growth factor I (IGF-I) receptors are partially purified from human placenta by sequential affinity chromatography with wheat germ agglutinin-agarose and agarose derivatized with an IGF-I analog. Sepharose 155-162 insulin like growth factor 1 Homo sapiens 0-28 3015966-2 1986 Insulin-like growth factor I (IGF-I) receptors are partially purified from human placenta by sequential affinity chromatography with wheat germ agglutinin-agarose and agarose derivatized with an IGF-I analog. Sepharose 155-162 insulin like growth factor 1 Homo sapiens 30-35 3015966-2 1986 Insulin-like growth factor I (IGF-I) receptors are partially purified from human placenta by sequential affinity chromatography with wheat germ agglutinin-agarose and agarose derivatized with an IGF-I analog. Sepharose 167-174 insulin like growth factor 1 Homo sapiens 0-28 3015966-2 1986 Insulin-like growth factor I (IGF-I) receptors are partially purified from human placenta by sequential affinity chromatography with wheat germ agglutinin-agarose and agarose derivatized with an IGF-I analog. Sepharose 167-174 insulin like growth factor 1 Homo sapiens 30-35 3015966-3 1986 Adsorption specificity to this affinity matrix demonstrates that low coupling ratios of IGF-I analog to agarose yield preparations that are highly selective in purifying IGF-I receptor with minimal cross-contamination by the insulin receptor present in the same placental extracts. Sepharose 104-111 insulin like growth factor 1 receptor Homo sapiens 170-184 3026338-1 1986 Human thrombomodulin, an endothelial-cell-membrane glycoprotein, has been purified from placenta by Triton X-100 extraction and by affinity chromatography on concanavalin A-Sepharose and thrombin-Sepharose. Sepharose 173-182 thrombomodulin Homo sapiens 6-20 3026338-1 1986 Human thrombomodulin, an endothelial-cell-membrane glycoprotein, has been purified from placenta by Triton X-100 extraction and by affinity chromatography on concanavalin A-Sepharose and thrombin-Sepharose. Sepharose 196-205 thrombomodulin Homo sapiens 6-20 3532418-2 1986 One fragment of 60 kdaltons elutes from gelatin-Sepharose, after intact fibronectin, with pH 5.5, 50 mM citrate, 0.1 M NaCl. Sepharose 48-57 fibronectin 1 Homo sapiens 72-83 3757265-2 1986 On two-dimensional Con A-containing agarose gel electrophoresis, the Kasahara isozyme was separated into three molecular species. Sepharose 36-43 alkaline phosphatase, intestinal Homo sapiens 69-85 3757265-4 1986 One of the three molecular species of Kasahara isozyme separated by Con A-containing agarose gel electrophoresis was extracted from the gel and applied to the polyacrylamide gradient gel electrophoresis again, resulting in the same electrophoretic pattern as that of the original Kasahara isozyme. Sepharose 85-92 alkaline phosphatase, intestinal Homo sapiens 38-54 3768895-4 1986 The fibronectin glycopeptides were purified by gel-permeation chromatography and Con A-Sepharose and were analyzed by anion-exchange chromatography and affinity columns of immobilized 5-hydroxytryptamine and lectins. Sepharose 87-96 fibronectin 1 Homo sapiens 4-15 2425853-2 1986 Affinity chromatography of the fraction with antithrombin III-agarose yielded two chondroitin sulfate proteoglycans of a non-binding (proteoglycan IA) and binding (proteoglycan IB) nature. Sepharose 62-69 serpin family C member 1 Homo sapiens 45-61 2426262-6 1986 The total rate of thrombin inhibition in plasma containing 4 mM free Ca2+ was of the order of 1.9 min-1, of which 0.4 min-1 was due to alpha 2M and 0.9 min-1 was due to inhibitors that were removed when plasma was passed through heparin-agarose. Sepharose 237-244 coagulation factor II, thrombin Homo sapiens 18-26 3730415-9 1986 Mouse hepatic lipase eluted from heparin-Sepharose at lower salt concentration than rat or human hepatic lipase, demonstrating that it has a relatively low affinity for heparin-like polysaccharides. Sepharose 41-50 lipase, hepatic Mus musculus 6-20 3800906-2 1986 These fragments did not exhibit direct thrombin-neutralizing activity; however, one unique fragment was found to bind to heparin-Sepharose and also to interfere with the inhibition of thrombin by intact antithrombin. Sepharose 129-138 coagulation factor II, thrombin Homo sapiens 184-192 3525545-10 1986 On the basis of its molecular weight and purification via immunoadsorption with protein A-Sepharose-bound anti-calmodulin IgG, this phosphoprotein was identified as a phosphorylated form of calmodulin. Sepharose 90-99 calmodulin 1 Rattus norvegicus 111-121 3525545-10 1986 On the basis of its molecular weight and purification via immunoadsorption with protein A-Sepharose-bound anti-calmodulin IgG, this phosphoprotein was identified as a phosphorylated form of calmodulin. Sepharose 90-99 calmodulin 1 Rattus norvegicus 190-200 3800906-2 1986 These fragments did not exhibit direct thrombin-neutralizing activity; however, one unique fragment was found to bind to heparin-Sepharose and also to interfere with the inhibition of thrombin by intact antithrombin. Sepharose 129-138 serpin family C member 1 Homo sapiens 203-215 2426695-5 1986 The estradiol receptor was able to bind to aprotinin-agarose only under transforming conditions; i.e., the interaction was hormone- and temperature-dependent and inhibited by molybdate. Sepharose 53-60 estrogen receptor 1 Homo sapiens 4-22 3013891-3 1986 A novel scheme based on reverse immunoaffinity chromatography using immobilized antibodies to membrane proteins from receptor down-regulated ovary and subsequent two-step affinity purification on hCG-Sepharose was used to isolate homogeneous receptor. Sepharose 200-209 hypertrichosis 2 (generalised, congenital) Homo sapiens 196-199 2942536-3 1986 Using an enzyme-linked immunosorbent assay and a TSP-Sepharose affinity bead-binding assay we have found that Plg-TSP complex formation was markedly enhanced (up to 5-fold) when catalytic concentrations of Plg activators were included in the reaction mixtures. Sepharose 53-62 thrombospondin 1 Homo sapiens 49-52 2942536-3 1986 Using an enzyme-linked immunosorbent assay and a TSP-Sepharose affinity bead-binding assay we have found that Plg-TSP complex formation was markedly enhanced (up to 5-fold) when catalytic concentrations of Plg activators were included in the reaction mixtures. Sepharose 53-62 plasminogen Homo sapiens 110-113 2942536-3 1986 Using an enzyme-linked immunosorbent assay and a TSP-Sepharose affinity bead-binding assay we have found that Plg-TSP complex formation was markedly enhanced (up to 5-fold) when catalytic concentrations of Plg activators were included in the reaction mixtures. Sepharose 53-62 thrombospondin 1 Homo sapiens 114-117 2942536-3 1986 Using an enzyme-linked immunosorbent assay and a TSP-Sepharose affinity bead-binding assay we have found that Plg-TSP complex formation was markedly enhanced (up to 5-fold) when catalytic concentrations of Plg activators were included in the reaction mixtures. Sepharose 53-62 plasminogen Homo sapiens 206-209 3017320-2 1986 We have purified the type 2 receptor from these cells by solubilization of crude membranes in Triton X-100, followed by chromatography on agarose-immobilized rIGF-II. Sepharose 138-145 insulin-like growth factor 2 Rattus norvegicus 158-165 2943315-7 1986 Also, stimulation of plasmin formation is not observed with dextran sulfate or chondroitin sulfate A, B, or C. Analyses of heparin fractions after separation on columns of antithrombin III-Sepharose suggest that both the high-affinity and the low-affinity fractions, which have dramatically different anticoagulant activity, have similar activity toward the fibrinolytic components. Sepharose 189-198 plasminogen Homo sapiens 21-28 3089784-1 1986 N-Glycosidically linked glycopeptides released by mild alkaline treatment of human factor VIII/von Willebrand factor (FVIII/vWF) were fractionated by serial affinity chromatography on columns of Sepharose linked to concanavalin A (ConA) and Lens culinaris agglutinin (LCA). Sepharose 195-204 coagulation factor VIII Homo sapiens 118-123 3487573-6 1986 The same selective changes in phospholipid metabolism were observed in neoplastic B lymphocytes (BCL1) after treatment with Sepharose anti-mu, but not with Sepharose anti-Ia or Sepharose normal Ig. Sepharose 124-133 cyclin D1 Mus musculus 97-101 3720668-4 1986 The major proenkephalin mRNA species expressed in the rat testis migrates as a 1900-nucleotide RNA on denaturing agarose gels. Sepharose 113-120 proenkephalin Rattus norvegicus 10-23 2944852-1 1986 The light chain of plasmin, prepared by selective reduction of the interchain disulfide bridges, can be separated from the heavy chain by affinity adsorption onto Kunitz inhibitor/Sepharose. Sepharose 180-189 plasminogen Homo sapiens 19-26 3091666-1 1986 N-Acetylglucosaminide beta 1, 4-galactosyltransferase (EC 2.4.1.22) was purified from rat milk by affinity chromatography on N-acetylglucosamine-Sepharose and alpha-lactalbumin-Sepharose columns. Sepharose 145-154 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 32-53 3091666-1 1986 N-Acetylglucosaminide beta 1, 4-galactosyltransferase (EC 2.4.1.22) was purified from rat milk by affinity chromatography on N-acetylglucosamine-Sepharose and alpha-lactalbumin-Sepharose columns. Sepharose 177-186 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 32-53 2943947-14 1986 GIF, with an apparent MW of 90K could be absorbed out on certain tumor cell lines or on Blue-Sepharose. Sepharose 93-102 metallothionein 3 Homo sapiens 0-3 3489143-3 1986 However, the BSF-1-response of CBA/N B cells was detected when activated by the anti-mu antibody-coupled Sepharose-beads. Sepharose 105-114 interleukin 4 Mus musculus 13-18 2943592-2 1986 Tissue plasminogen activator was treated with Sepharose-bound trypsin or chymotrypsin. Sepharose 46-55 chromosome 20 open reading frame 181 Homo sapiens 0-28 3722172-7 1986 BP, quantitated using a specific radioimmunoassay, was retained by agarose-immobilized IGF-I, IGF-II, concanavalin A, and wheat germ lectin, but not Helix pomatia lectin. Sepharose 67-74 insulin like growth factor 1 Homo sapiens 87-92 3722172-7 1986 BP, quantitated using a specific radioimmunoassay, was retained by agarose-immobilized IGF-I, IGF-II, concanavalin A, and wheat germ lectin, but not Helix pomatia lectin. Sepharose 67-74 insulin like growth factor 2 Homo sapiens 94-100 3755134-4 1986 Purified fragments were then applied to a column of HCII bound to concanavalin A-Sepharose, and bound oligosaccharides were eluted with a gradient of sodium chloride. Sepharose 81-90 serpin family D member 1 Homo sapiens 52-56 3487353-2 1986 However, in contrast to the latter two disorders in which the larger vWF multimers are absent in plasma, the entire range of vWF multimers was observed in the patients" plasma after sodium dodecyl sulfate-agarose gel electrophoresis, and all vWF multimers (including the largest) were present in the same proportion as in normal plasma and type I vWD. Sepharose 205-212 von Willebrand factor Homo sapiens 125-128 3487353-2 1986 However, in contrast to the latter two disorders in which the larger vWF multimers are absent in plasma, the entire range of vWF multimers was observed in the patients" plasma after sodium dodecyl sulfate-agarose gel electrophoresis, and all vWF multimers (including the largest) were present in the same proportion as in normal plasma and type I vWD. Sepharose 205-212 von Willebrand factor Homo sapiens 125-128 3533405-1 1986 A procollagen C-proteinase was purified about 100-fold from the medium of cultured mouse fibroblasts by a combination of ammonium sulfate precipitation, gel-filtration, and affinity chromatography on a column of Sepharose coupled to the carboxyl propeptide of type I procollagen. Sepharose 212-221 bone morphogenetic protein 1 Mus musculus 2-26 3088041-2 1986 The A chain was found to be responsible for the binding to lysine-Sepharose or fibrin and the B chain contained the catalytic activity of tissue-type plasminogen activator. Sepharose 66-75 plasminogen activator, tissue type Homo sapiens 138-171 3748278-5 1986 Finally, mChAT was applied to a CoA-Sepharose column equilibrated with buffer containing 100 mM choline chloride and was specifically eluted with buffer containing acetyl-CoA. Sepharose 36-45 choline acetyltransferase Mus musculus 9-14 3748278-11 1986 Furthermore, as previously reported for soluble ChAT, Fab-Sepharose chromatography could be used for the purification of mChAT and this preparation also resolved into two bands on 10% SDS gel. Sepharose 58-67 choline acetyltransferase Mus musculus 48-52 3748278-11 1986 Furthermore, as previously reported for soluble ChAT, Fab-Sepharose chromatography could be used for the purification of mChAT and this preparation also resolved into two bands on 10% SDS gel. Sepharose 58-67 choline acetyltransferase Mus musculus 121-126 3750265-1 1986 Human thrombin with high affinity for fibrin was obtained by subjecting purified thrombin to affinity chromatography on Sepharose insolubilized fibrin monomers, after addition of a radioiodinated subsample of thrombin, molar ratio 1:600. Sepharose 120-129 coagulation factor II, thrombin Homo sapiens 6-14 3722822-8 1986 Chromatography of serum on TNP-Sepharose provided a efficient and simple way of purifying SAP. Sepharose 31-40 amyloid P component, serum Homo sapiens 90-93 3011795-1 1986 Insulin-like growth factor (IGF) I receptor was purified from Triton X-100-solubilized human placental membranes by wheat germ agglutinin-Sepharose chromatography followed by immunoaffinity chromatography using alpha IR-3, a monoclonal antibody directed against the IGF-I receptor. Sepharose 138-147 insulin like growth factor 1 receptor Homo sapiens 0-43 3011795-2 1986 Purification of 3200-fold and 2800-fold was achieved from wheat germ agglutinin-Sepharose eluates with regard to IGF-I binding and kinase activities. Sepharose 80-89 insulin like growth factor 1 Homo sapiens 113-118 3086313-4 1986 As revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by fibrin-agarose zymography, incubation of HT-1080-inhibitor with the active form of human u-PA led to the formation of an equimolar sodium dodecyl sulfate-resistant complex between them; in contrast, no complex formation was observed between the inhibitor and the proenzyme form of human u-PA (pro-u-PA). Sepharose 92-99 plasminogen activator, urokinase Homo sapiens 174-178 3711123-1 1986 We have purified the membrane-intrinsic glycerol-3-phosphate dehydrogenase from both normal and hyperthyroid rat liver mitochondria by extraction with Triton X-100, hydrophobic affinity chromatography, ion exchange chromatography, gel filtration, and FAD-linked Sepharose 4B affinity chromatography. Sepharose 262-271 glycerol-3-phosphate dehydrogenase 1 Rattus norvegicus 40-74 2939882-1 1986 A proteinase inhibitor has been isolated from human colorectal adenocarcinomas by extraction with a low-ionic-strength buffer and a combination of Con A-Sepharose, Sephadex G-200, DEAE-cellulose and chromatofocusing steps. Sepharose 153-162 endogenous retrovirus group K member 18 Homo sapiens 2-12 3015126-1 1986 The type I IGF receptor from human placental membranes was purified to near homogeneity by affinity chromatography on IGF I-Sepharose. Sepharose 124-133 insulin like growth factor 1 Homo sapiens 118-123 3090079-1 1986 Thyroxine-binding globulin was isolated from pooled human serum by a two-step method involving affinity chromatography on thyroxine-Sepharose and preparative disc-electrophoresis. Sepharose 132-141 serpin family A member 7 Homo sapiens 0-26 3085725-1 1986 Poly(ADPribose) synthetase has been purified to apparent homogeneity from sheep testis by a simple procedure using three chromatographic steps (DNA-agarose, blue Sephadex G-150 and phosphocellulose P11). Sepharose 148-155 poly [ADP-ribose] polymerase 1 Ovis aries 0-26 3711098-3 1986 Sepharose CL-6B gel filtration removes residual protein contaminants and transfers asialo-gpL115 from Nonidet P-40-containing to sodium dodecyl sulfate-containing buffer. Sepharose 0-9 sialophorin Homo sapiens 90-96 3711098-3 1986 Sepharose CL-6B gel filtration removes residual protein contaminants and transfers asialo-gpL115 from Nonidet P-40-containing to sodium dodecyl sulfate-containing buffer. Sepharose 0-9 interleukin 9 Homo sapiens 110-114 3754869-5 1986 Complex formation between S-protein and thrombin, and between S-protein, thrombin, and ATIII, was demonstrated by agarose gel electrophoresis and by two-dimensional immunoelectrophoresis of purified proteins and in recalcified, clotted plasma. Sepharose 114-121 vitronectin Homo sapiens 26-35 3754869-5 1986 Complex formation between S-protein and thrombin, and between S-protein, thrombin, and ATIII, was demonstrated by agarose gel electrophoresis and by two-dimensional immunoelectrophoresis of purified proteins and in recalcified, clotted plasma. Sepharose 114-121 coagulation factor II, thrombin Homo sapiens 40-48 3754869-5 1986 Complex formation between S-protein and thrombin, and between S-protein, thrombin, and ATIII, was demonstrated by agarose gel electrophoresis and by two-dimensional immunoelectrophoresis of purified proteins and in recalcified, clotted plasma. Sepharose 114-121 vitronectin Homo sapiens 62-71 3754869-5 1986 Complex formation between S-protein and thrombin, and between S-protein, thrombin, and ATIII, was demonstrated by agarose gel electrophoresis and by two-dimensional immunoelectrophoresis of purified proteins and in recalcified, clotted plasma. Sepharose 114-121 coagulation factor II, thrombin Homo sapiens 73-81 3754869-5 1986 Complex formation between S-protein and thrombin, and between S-protein, thrombin, and ATIII, was demonstrated by agarose gel electrophoresis and by two-dimensional immunoelectrophoresis of purified proteins and in recalcified, clotted plasma. Sepharose 114-121 serpin family C member 1 Homo sapiens 87-92 3708154-0 1986 Purification of Hageman factor (factor XII) on columns of popcorn-agarose. Sepharose 66-73 coagulation factor XII Homo sapiens 16-30 3708154-4 1986 Rapid purification of HF was achieved by using as the major purification step adsorption of this clotting factor to popcorn inhibitor bound to agarose. Sepharose 143-150 coagulation factor XII Homo sapiens 22-24 3753469-1 1986 Mouse brain ornithine decarboxylase (ODC) was purified to near-homogeneity by using (NH4)2SO4 precipitation and chromatography on heparin-Sepharose, pyridoxamine phosphate-agarose and DEAE-cellulose. Sepharose 138-147 ornithine decarboxylase, structural 1 Mus musculus 37-40 3753469-4 1986 By affinity chromatography on ODC-bound Sepharose, the extra enzyme activity was shown to originate, at least partly, from the enzyme-antizyme complex. Sepharose 40-49 ornithine decarboxylase, structural 1 Mus musculus 30-33 3708816-3 1986 In contrast, human gamma- and bovine beta-thrombins, made by controlled passage of alpha-thrombin through trypsin agarose, were greater than 98% of their respective forms and had fibrinogen-clotting activity less than 1 kcu/g. Sepharose 114-121 coagulation factor II, thrombin Bos taurus 42-50 3758662-3 1986 The actin-like protein was identified by the method of affinity binding on DNAse1-sepharose and by electrophoresis on polyacrylamide gel with sodium dodecyl sulphate. Sepharose 82-91 deoxyribonuclease 1 Rattus norvegicus 75-81 2428746-1 1986 The binding site specificity of a carbohydrate-binding protein (CBP) synthesized in vitro by normal human peripheral leucocytes was analyzed by affinity chromatography on asialofetuin-Sepharose. Sepharose 184-193 CREB binding protein Homo sapiens 34-62 2428746-1 1986 The binding site specificity of a carbohydrate-binding protein (CBP) synthesized in vitro by normal human peripheral leucocytes was analyzed by affinity chromatography on asialofetuin-Sepharose. Sepharose 184-193 CREB binding protein Homo sapiens 64-67 3710738-2 1986 Glucose 6-phosphate dehydrogenase was purified 450-fold with a yield of 91% by anion exchange chromatography and 2",5"-ADP agarose affinity chromatography. Sepharose 123-130 glucose-6-phosphate dehydrogenase Bos taurus 0-33 3084650-6 1986 Because anti-CD3-Sepharose did not cause modulation of the CD3 antigen, the ability of the Sepharose-bound antibody to induce T cells to express IL 2 receptors and to respond to individual second signals may be related to lack of modulation rather than valency of binding. Sepharose 91-100 interleukin 2 Homo sapiens 145-149 3486890-6 1986 We isolated individual vWf oligomer species from the agarose gel bands and show that vWf minor, or satellite, species differ from major species in subunit composition. Sepharose 53-60 von Willebrand factor Homo sapiens 23-26 3528366-4 1986 Residual apoE and albumin, amounting up to 0.5% of the apoB mass, were resistant to removal by TGRP treatment as well as by heparin-Sepharose column chromatography. Sepharose 132-141 apolipoprotein E Homo sapiens 9-13 3745988-3 1986 Two IFN species, one with a weakly ionic and the other with a hydrophobic interaction with Blue Sepharose, exhibited heterologous antiviral activity, and were acid- and heat-labile. Sepharose 96-105 interferon alpha 1 Homo sapiens 4-7 3745988-6 1986 The remaining IFN species, with a weakly ionic interaction with Blue Sepharose, was acid- and heat-stable, exhibited no heterologous cell antiviral activity, and was not neutralized by any antisera to IFN. Sepharose 69-78 interferon alpha 1 Homo sapiens 14-17 3736037-0 1986 Androgen receptor affinity chromatography: synthesis and properties of 17 alpha-epoxypropyl-dihydrotestosterone Sepharose. Sepharose 112-121 androgen receptor Rattus norvegicus 0-17 3526144-7 1986 Fibronectin receptor was purified using affinity chromatography on human fibronectin coupled to Sepharose. Sepharose 96-105 fibronectin 1 Homo sapiens 0-11 3526144-9 1986 The purified fibronectin receptor was active since 40-60% of labeled receptor could rebind to fibronectin-Sepharose. Sepharose 106-115 fibronectin 1 Homo sapiens 13-24 3526144-9 1986 The purified fibronectin receptor was active since 40-60% of labeled receptor could rebind to fibronectin-Sepharose. Sepharose 106-115 fibronectin 1 Homo sapiens 94-105 16664862-8 1986 Blue Sepharose columns loaded with NR extract of urea-grown mutants and sequentially eluted with NADPH and NADH yielded a NADPH:NR peak only, while the wild-type yielded both NADPH: and NADH:NR peaks. Sepharose 5-14 inducible nitrate reductase [NADH] 1 Glycine max 35-37 16664862-8 1986 Blue Sepharose columns loaded with NR extract of urea-grown mutants and sequentially eluted with NADPH and NADH yielded a NADPH:NR peak only, while the wild-type yielded both NADPH: and NADH:NR peaks. Sepharose 5-14 inducible nitrate reductase [NADH] 1 Glycine max 128-130 16664862-8 1986 Blue Sepharose columns loaded with NR extract of urea-grown mutants and sequentially eluted with NADPH and NADH yielded a NADPH:NR peak only, while the wild-type yielded both NADPH: and NADH:NR peaks. Sepharose 5-14 inducible nitrate reductase [NADH] 1 Glycine max 128-130 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 106-113 insulin Homo sapiens 0-7 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 0-7 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 118-125 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 283-290 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin-like growth factor 1 Rattus norvegicus 313-318 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 0-7 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 118-125 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin Homo sapiens 283-290 3012553-1 1986 Insulin receptors purified from human placenta by sequential affinity chromatography on wheat germ lectin-agarose and insulin coupled to 1,1"-carbonyldiimidazole-activated agarose (CDI-agarose) retained full binding activity but bound a greater than predicted amount of 125I-labeled insulin-like growth factor I (IGF-I). Sepharose 172-179 insulin-like growth factor 1 Rattus norvegicus 313-318 24232027-1 1986 Phosphoenolpyruvate carboxylase (EC 4.1.1.31) was purified 43-fold from Amaranthus viridis leaves by using a combination of ammonium-sulphate fractionation, chromatography on O-(diethylaminoethyl)-cellulose and hydroxylapatite, and filtration through Sepharose 6B. Sepharose 251-260 MLO-like protein 4 Zea mays 0-31 3700427-6 1986 At pH 7.4, calmodulin N-methyltransferase did not bind to DEAE-cellulose, but bound strongly to CM-Sepharose. Sepharose 99-108 calmodulin 1 Rattus norvegicus 11-21 3707976-2 1986 Lipoprotein lipase was purified by heparin-Sepharose affinity chromatography followed by preparative isoelectric focusing. Sepharose 43-52 lipoprotein lipase Rattus norvegicus 0-18 2940939-2 1986 The new procedure, using adsorption chromatography on hydroxylapatite crystals immobilized within a crosslinked agarose gel (HA-Ultrogel), is simple and reproducibly provides a high yield of band 4.1 which is essentially free of protein kinase. Sepharose 112-119 erythrocyte membrane protein band 4.1 Homo sapiens 191-199 3700402-6 1986 The dissociated subunits can be separated by affinity chromatography on Sepharose-rabbit IgG anti-human beta 2-microglobulin and reassociate in vitro when incubated under the appropriate conditions. Sepharose 72-81 beta-2-microglobulin Homo sapiens 104-124 3790076-2 1986 This LPL-like activity is eluted by 1.5 M-NaCl from heparin-Sepharose columns. Sepharose 60-69 lipoprotein lipase Rattus norvegicus 5-8 3715811-0 1986 Binding properties on Sepharose insolubilized fibrinogen and fibrin, of various species of fibrinogen and fibrin solubilized in plasma. Sepharose 22-31 fibrinogen beta chain Homo sapiens 46-56 3715811-0 1986 Binding properties on Sepharose insolubilized fibrinogen and fibrin, of various species of fibrinogen and fibrin solubilized in plasma. Sepharose 22-31 fibrinogen beta chain Homo sapiens 91-101 3715811-6 1986 Sepharose insolubilized fibrinogen favoured the adsorption of soluble fibrins as compared to fibrinogen in solution; the adsorption of soluble des-AA fibrin was similar to that of soluble des-AABB fibrin. Sepharose 0-9 fibrinogen beta chain Homo sapiens 24-34 3755043-1 1986 Two molecular forms of prostatropin distributed among five chromatographic peaks have been isolated from bovine brain by heparin-Sepharose affinity and reverse phase high performance liquid chromatography. Sepharose 129-138 fibroblast growth factor 1 Bos taurus 23-35 2871018-1 1986 An atrial natriuretic factor (ANF) receptor from rat lung was solubilized with Lubrol-PX and purified by sequential chromatographic steps on GTP-agarose, DEAE-Sephacel, phenyl-agarose, and wheat germ agglutinin-agarose. Sepharose 145-152 natriuretic peptide A Rattus norvegicus 30-33 3697377-8 1986 Triton X-100 extracts freshly prepared from intestinal microvilli essentially showed one form of trehalase, which behaved on phenyl-Sepharose and Con A-Sepharose chromatography in the same manner as purified amphiphilic trehalase. Sepharose 132-141 trehalase Oryctolagus cuniculus 97-106 3753440-1 1986 Human cathepsin B was purified by affinity chromatography on the semicarbazone of Gly-Phe-glycinal linked to Sepharose 4B, with elution by 2,2"-dipyridyl disulphide at pH 4.0. Sepharose 109-118 cathepsin B Homo sapiens 6-17 3753448-10 1986 PGI and PGII, but neither PGIII nor PGIV, were bound to LDL-Sepharose. Sepharose 60-69 decorin Homo sapiens 8-12 3753448-11 1986 The main peaks of PGI and PGII were eluted from LDL-Sepharose with 60 mM- and 90 mM-NaCl respectively. Sepharose 52-61 decorin Homo sapiens 26-30 3710480-1 1986 Using "new" techniques (malic acid thin layer agarose gel electrophoresis and/or isoelectric focusing), the polymorphism of the human red cell isozyme system esterase D (ESD) was shown to be extended. Sepharose 46-53 esterase D Homo sapiens 158-168 3710480-1 1986 Using "new" techniques (malic acid thin layer agarose gel electrophoresis and/or isoelectric focusing), the polymorphism of the human red cell isozyme system esterase D (ESD) was shown to be extended. Sepharose 46-53 esterase D Homo sapiens 170-173 3722000-4 1986 In the course of studying the heterogeneity of rat androgen binding protein on Concanavalin A-Sepharose, it was observed that the distribution of the two forms of this protein was similar in the fluid obtained by micropuncture from the seminiferous tubule and the rete testis, that is, the ratios of Form I to Form II were 1:1 and 1:1.8, respectively. Sepharose 94-103 sex hormone binding globulin Rattus norvegicus 51-75 2937840-1 1986 Glycosylation inhibiting factor (GIF) was purified from culture filtrates of a T cell hybridoma, 23A4, by affinity chromatography on anti-lipomodulin Sepharose. Sepharose 150-159 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 0-31 2937840-1 1986 Glycosylation inhibiting factor (GIF) was purified from culture filtrates of a T cell hybridoma, 23A4, by affinity chromatography on anti-lipomodulin Sepharose. Sepharose 150-159 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 33-36 3709960-8 1986 Treatment of the hCG-activated fluid with anti-hCG gamma-globulin Sepharose did not abolish the permeability effect of the fluid. Sepharose 66-75 hypertrichosis 2 (generalised, congenital) Homo sapiens 17-20 3520539-1 1986 Thyroglobulin was isolated from the cattle thyroid gland by chromatography on Sephadex G-200 and Sepharose 4B. Sepharose 97-109 thyroglobulin Bos taurus 0-13 3520539-3 1986 However, gel filtration of thyroglobulin incubated at 37 degrees through Sepharose 6B revealed that it contained proteinases of the serine type. Sepharose 73-82 thyroglobulin Bos taurus 27-40 3010299-1 1986 A restriction fragment length polymorphism at the human c-abl locus (ABL) has been detected in 67 unrelated individuals by agarose gel electrophoresis and Southern blot hybridization using 32P-labeled v-abl probes. Sepharose 123-130 ABL proto-oncogene 1, non-receptor tyrosine kinase Homo sapiens 56-61 3010299-1 1986 A restriction fragment length polymorphism at the human c-abl locus (ABL) has been detected in 67 unrelated individuals by agarose gel electrophoresis and Southern blot hybridization using 32P-labeled v-abl probes. Sepharose 123-130 ABL proto-oncogene 1, non-receptor tyrosine kinase Homo sapiens 69-72 3458191-3 1986 These two species can be separated by immunosorption on columns of anti-apolipoprotein E bound to Sepharose. Sepharose 98-107 apolipoprotein E Oryctolagus cuniculus 72-88 3761627-0 1986 [Analysis of guanase by agarose gel electrophoresis]. Sepharose 24-31 guanine deaminase Homo sapiens 13-20 3715809-1 1986 Of four Tris-salts tested (chloride, sulfate, phosphate and acetate), chloride caused complete elution of antithrombin III (AT III) from a heparin-Sepharose column and sulfate caused partial elution. Sepharose 147-156 serpin family C member 1 Homo sapiens 106-122 3715809-1 1986 Of four Tris-salts tested (chloride, sulfate, phosphate and acetate), chloride caused complete elution of antithrombin III (AT III) from a heparin-Sepharose column and sulfate caused partial elution. Sepharose 147-156 serpin family C member 1 Homo sapiens 124-130 2424117-4 1986 One antibody (F37), which reacted with both free and complex-bound inhibitor I, was used for further study by a method involving binding of the antibody to protein A-Sepharose, immunoadsorption of antigen and analysis of the resulting supernatant by SDS-polyacrylamide gel electrophoresis and enzymography. Sepharose 166-175 leucine zipper tumor suppressor 1 Homo sapiens 14-17 2944301-1 1986 The ability of calcium loaded heavy sarcoplasmic reticulum vesicles to specifically respond to the addition of various agents such as caffeine, calcium ions and calmodulin antagonists to rapidly released calcium can largely be diminished by passing the vesicular suspension it 0.3 M sucrose, 0.6 M KCl, 4 mM CaCl2, pH 7.0 through a Sepharose 6B column or by centrifuging it through a sucrose gradient prepared with the same salt medium. Sepharose 332-341 calmodulin 1 Homo sapiens 161-171 3085986-2 1986 In this immunologic technique, glass capillaries filled with agarose gel containing anti-Apo A-I are used. Sepharose 61-68 apolipoprotein A1 Homo sapiens 89-96 3520939-2 1986 The method is based upon: 1) Separation of the vWF multimers by SDS-agarose electrophoresis, 2) Subsequent blotting of the vWF multimers onto nitrocellulose, 3) Immunolocalization and visualization of the vWF pattern by the sequential incubation of the blot with primary vWF antiserum, peroxidase- or beta-galactosidase-conjugated secondary antibodies and a relevant chromogenic substrate. Sepharose 68-75 von Willebrand factor Homo sapiens 47-50 3707588-4 1986 Furthermore, after removal of CCK from the plasma by immunoabsorption with an OAL-656-bound Sepharose 4B column, the stimulatory effect of the plasma on pancreatic enzyme secretion was abolished when it was injected intravenously into recipient rats. Sepharose 92-101 cholecystokinin Rattus norvegicus 30-33 3715788-5 1986 When tested by affinity chromatography on heparin Sepharose, this abnormal AT III did not bind to heparin. Sepharose 50-59 serpin family C member 1 Homo sapiens 75-81 3517539-4 1986 PNMT was purified by sequential pH 5.0 treatment and dialysis and by column chromatographic procedures using DEAE-Sephacel, Sephacryl S-200 and Phenyl Boronate-agarose. Sepharose 160-167 phenylethanolamine N-methyltransferase Homo sapiens 0-4 3698098-3 1986 Data obtained with plasmids immobilized in agarose indicate that TFIIIA finds its site via a DNA-mediated transfer mechanism, and that all-or-none gyration is a consequence of TFIIIA transfer between 5S DNA sites. Sepharose 43-50 general transcription factor 3A L homeolog Xenopus laevis 65-71 3707929-4 1986 As a midcourse check on the progress of purification, we translated a small portion of the purified Cat2 mRNA-containing polysomes while they were still complexed with CAT-2 antibodies and bound to protein A-Sepharose. Sepharose 208-217 catalase isozyme 2 Zea mays 100-104 3707929-8 1986 We have also enriched for Cat2 mRNA by size selection on methylmercury-agarose gels. Sepharose 71-78 catalase isozyme 2 Zea mays 26-30 3009181-1 1986 Purification of alpha 2-plasmin inhibitor (alpha 2PI) from human plasma by affinity chromatography on plasminogen-Sepharose resulted in copurification of a contaminating protein with Mr 17,000 as judged by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. Sepharose 114-123 serpin family F member 2 Homo sapiens 16-41 3009181-1 1986 Purification of alpha 2-plasmin inhibitor (alpha 2PI) from human plasma by affinity chromatography on plasminogen-Sepharose resulted in copurification of a contaminating protein with Mr 17,000 as judged by sodium dodecyl sulphate/polyacrylamide gel electrophoresis. Sepharose 114-123 serpin family F member 2 Homo sapiens 43-52 3009181-3 1986 The use of the kringle 1-3 part of plasminogen, K(1 + 2 + 3), bound to Sepharose for affinity chromatography, instead of plasminogen-Sepharose, resulted in an alpha 2PI preparation without this contaminant. Sepharose 71-80 serpin family F member 2 Homo sapiens 159-168 3957921-6 1986 A ganglioside-Sepharose affinity column has been constructed which specifically binds the 31,000-dalton amino terminal fragment of fibronectin. Sepharose 14-23 fibronectin 1 Homo sapiens 131-142 3964300-6 1986 N-Acylurea albumin and N-acylurea transferrin at low concentrations retarded the migration of lambda-Pstl restriction fragments, pBR322 plasmid and M13 mp8 single-stranded DNA on agarose gels, while at higher concentrations of modified protein the N-acylurea protein-DNA complexes were unable to enter the gel. Sepharose 179-186 serotransferrin Bos taurus 34-45 2423076-2 1986 After acidification of human plasma and separation of insulin-like growth factor I and endogenous binding protein by cation exchange chromatography on SP-Sephadex the material was passed through a column packed with pure human amniotic fluid binding protein covalently coupled to Sepharose. Sepharose 280-289 insulin like growth factor binding protein 1 Homo sapiens 227-257 3707569-4 1986 Further purification of Cibacron blue purified EDGF with heparin sepharose chromatography yielded two active fractions after elution with a sodium chloride gradient. Sepharose 65-74 fibroblast growth factor 1 Bos taurus 47-51 2420791-6 1986 Like the membrane-bound ER alpha-mannosidase, the soluble alpha-mannosidase can hydrolyze alpha-linked mannose from both p-nitrophenyl alpha-mannoside (Km = 0.14 mM) and high mannose oligosaccharides, is not inhibited by the mannose analogues swainsonine and 1-deoxymannojirimycin, is stabilized by MnCl2 or CoCl2, and does not bind to concanavalin A-Sepharose. Sepharose 351-360 estrogen receptor 1 Rattus norvegicus 24-32 3007451-5 1986 The gelatinase co-purified with fibronectin in chromatography on Sepharoses conjugated with gelatin, arginine, and heparin but could be separated from fibronectin by gel filtration in a physiological buffer. Sepharose 65-75 fibronectin 1 Homo sapiens 32-43 3082868-13 1986 This was done by first substituting agarose-bound calmodulin for free calmodulin in the reactivation procedure described above and, then, elution of the gamma subunit from the calmodulin-agarose with a solution containing 1.0 M Tris-Cl (pH 7.0), 1% Triton X-100, 1 mM EGTA, and 5 mM dithiothreitol. Sepharose 36-43 calmodulin 1 Homo sapiens 50-60 3957914-8 1986 The elevated kinase activity of the insulin receptor derived from insulin-treated cells is not due to the presence of hormone bound to the receptor because the receptor kinase activity is assayed while immobilized on insulin-agarose. Sepharose 225-232 insulin receptor Rattus norvegicus 36-52 3518535-2 1986 A luminescent immunosorbent constituted of bacterial luciferase, FMN oxidoreductase, and an antibody coimmobilized on Sepharose is used to determine specifically the label enzyme (glucose-6-phosphate dehydrogenase, coupled to an antigen) bound by a specific antibody. Sepharose 118-127 glucose-6-phosphate dehydrogenase Homo sapiens 180-213 3007145-2 1986 Human C1s proenzyme (Mr 83 000) was isolated by a rapid two-stage method involving affinity chromatography of C1 on IgG-Sepharose and isolation of subcomponent C1s by ion-exchange chromatography on DEAE-Sephacel. Sepharose 120-129 complement C1s Homo sapiens 6-9 3514245-3 1986 These acidic species were co-purified with beta and gamma actins using DNase I-Sepharose affinity chromatography on the nuclear matrix. Sepharose 79-88 deoxyribonuclease I Mus musculus 71-78 3744442-1 1986 A carbohydrate-binding protein (CBP) synthesized in vitro by normal human peripheral leucocytes was isolated by affinity chromatography on asialofetuin-Sepharose. Sepharose 152-161 CREB binding protein Homo sapiens 2-30 3744442-1 1986 A carbohydrate-binding protein (CBP) synthesized in vitro by normal human peripheral leucocytes was isolated by affinity chromatography on asialofetuin-Sepharose. Sepharose 152-161 CREB binding protein Homo sapiens 32-35 3723019-0 1986 Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis. Sepharose 42-49 apolipoprotein B Homo sapiens 14-30 3723019-4 1986 We have developed an agarose-acrylamide gel electrophoretic method to separate the two major apoB forms. Sepharose 21-28 apolipoprotein B Homo sapiens 93-97 3458207-2 1986 The hepatoma-derived growth factor (HDGF) has been purified to homogeneity by a combination of Bio-Rex 70, heparin-Sepharose, and reverse-phase chromatography; it is a cationic polypeptide with a molecular weight of about 18,500-19,000. Sepharose 115-124 heparin binding growth factor Homo sapiens 4-34 3458207-2 1986 The hepatoma-derived growth factor (HDGF) has been purified to homogeneity by a combination of Bio-Rex 70, heparin-Sepharose, and reverse-phase chromatography; it is a cationic polypeptide with a molecular weight of about 18,500-19,000. Sepharose 115-124 heparin binding growth factor Homo sapiens 36-40 3484681-4 1986 IL-1-containing fractions were further analyzed by chromatography with DEAE-Sephacel, phenyl:Sepharose, and concanavalin A:Sepharose. Sepharose 93-102 interleukin 1 alpha Homo sapiens 0-4 3088754-6 1986 The extent of binding of the two pro-urokinases and their plasmin-activated forms to fibrin-sepharose decreased in the following order: 55 kd pro-urokinase 30 kd pro-urokinase 55 kd urokinase 30 kd urokinase. Sepharose 92-101 plasminogen Homo sapiens 58-65 3005328-4 1986 The apo-B,E(LDL) receptor was isolated by affinity chromatography on LDL-Sepharose. Sepharose 73-82 apolipoprotein B Homo sapiens 4-9 3005328-5 1986 The apo-E receptor, which did not bind to the LDL-Sepharose column, was then purified by using an HDLc (cholesterol-induced high density lipoprotein)-Sepharose affinity column and an immunoaffinity column. Sepharose 150-159 apolipoprotein E Homo sapiens 4-9 3081040-5 1986 Chromatography on 6% agarose showed that lymph apolipoproteins A-I and A-IV are present on triacylglycerol-rich particles and on particles of the size of HDL. Sepharose 21-28 apolipoprotein A1 Rattus norvegicus 47-75 3081046-5 1986 Coupled to agarose, this antibody allowed complete removal of apolipoprotein B-containing lipoproteins from normolipidemic, hypertriglyceridemic and hypercholesterolemic plasma. Sepharose 11-18 apolipoprotein B Homo sapiens 62-78 2419721-2 1986 The lipase binds to Sepharose-heparin columns from which it can be eluted by 0.8 to 0.9 M NaCl. Sepharose 20-29 lipase G, endothelial type Rattus norvegicus 4-10 3964668-3 1986 Residual globulins were removed by adsorption of the lectin on concanavalin A-agarose and elution with methyl alpha-mannoside. Sepharose 78-85 LOW QUALITY PROTEIN: lectin Glycine max 53-59 2419331-2 1986 8-Bromo-cAMP (1 mM) or forskolin (10 microM) enhanced the phosphorylation of the insulin receptor purified from 32P-labeled cells by affinity chromatography on wheat germ agglutinin-agarose and immunoprecipitation with monoclonal antibody. Sepharose 182-189 insulin Homo sapiens 81-88 3005045-1 1986 Concanavalin A-agarose treatment of rat liver post-mitochondrial supernatant removes a fraction rich in cholesterol and 5"-nucleotidase activity but low in glucose-6-phosphatase. Sepharose 15-22 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 156-177 3007126-1 1986 Various lipids were tested as substrates for the insulin receptor kinase using either receptor partially purified from rat hepatoma cells by wheat-germ-agglutinin-Sepharose chromatography or receptor purified from human placenta by insulin-Sepharose affinity chromatography. Sepharose 163-172 insulin receptor Homo sapiens 49-65 3007126-1 1986 Various lipids were tested as substrates for the insulin receptor kinase using either receptor partially purified from rat hepatoma cells by wheat-germ-agglutinin-Sepharose chromatography or receptor purified from human placenta by insulin-Sepharose affinity chromatography. Sepharose 240-249 insulin receptor Homo sapiens 49-65 3484979-1 1986 We identified a consecutive series of 12 children with noncyanotic congenital cardiac lesions with loss of the largest plasma von Willebrand factor (vWF) multimers determined by SDS-agarose electrophoresis. Sepharose 182-189 von Willebrand factor Homo sapiens 126-147 3484979-1 1986 We identified a consecutive series of 12 children with noncyanotic congenital cardiac lesions with loss of the largest plasma von Willebrand factor (vWF) multimers determined by SDS-agarose electrophoresis. Sepharose 182-189 von Willebrand factor Homo sapiens 149-152 3634630-1 1986 A relatively simple procedure for isolation and purification of human blood plasma kallikrein (HPK) by QAE-Sephadex A-50 SP-Sephadex C-50 and affinity chromatography on Sepharose 4B with immobilized soybean trypsin inhibitor with the activity yield of about 40% has been developed. Sepharose 169-181 kallikrein B1 Homo sapiens 76-93 3484681-4 1986 IL-1-containing fractions were further analyzed by chromatography with DEAE-Sephacel, phenyl:Sepharose, and concanavalin A:Sepharose. Sepharose 123-132 interleukin 1 alpha Homo sapiens 0-4 2420610-2 1986 This IgG2a antibody, either in soluble form or covalently coupled to Sepharose beads, can activate resting T cells from naive animals to proliferate. Sepharose 69-78 immunoglobulin heavy variable V1-9 Mus musculus 5-10 3699045-4 1986 A second fraction, designated BF1, adhered to concanavalin A-Sepharose exclusively through its carbohydrate recognition site. Sepharose 61-70 MHC BF1 class I Gallus gallus 30-33 2420701-4 1986 Soybean trypsin inhibitor coupled to Sepharose 4B was capable of absorbing the IL-2 degrading activity from the trypsin solution. Sepharose 37-49 interleukin 2 Homo sapiens 79-83 2936685-5 1986 The suppressive effect of TsF1 was removed by adsorption with intact heat-killed cryptococci and by SPIA on CneF-Sepharose 4B. Sepharose 113-125 serine/threonine kinase 16 Homo sapiens 26-30 2936685-6 1986 The binding of cryptococcal TsF1 to the cryptococcal SPIA column was shown to be specific since Sepharose 4B columns either coupled with Saccharomyces cerevisiae mannan or blocked with glycine did not adsorb the suppressor activity. Sepharose 96-105 serine/threonine kinase 16 Homo sapiens 28-32 3011762-3 1986 Three multiple forms of human myeloperoxidase were separated from the crystalline enzyme by CM-Sepharose chromatography with sodium chloride gradient elution. Sepharose 95-104 myeloperoxidase Homo sapiens 30-45 2936763-6 1986 When analyzed by polyacrylamide gel electrophoresis and Sepharose CL6B chromatography, the 440,000-D vWF oligomer is a dimer of the 220,000 subunit of fully reduced native vWF. Sepharose 56-65 von Willebrand factor Homo sapiens 101-104 3512602-4 1986 Purification of the patient"s plasma AT-III by heparin-sepharose affinity chromatography showed a normal protein recovery and elution profile, but the purified AT-III fraction showed only 50% of the normal progressive thrombin neutralization and anti-Xa activity. Sepharose 55-64 serpin family C member 1 Homo sapiens 37-43 3525581-5 1986 Both of these antibodies were purified by affinity chromatography on columns of fibronectin-Sepharose and were then incubated with soluble fibronectin to form antigen-antibody complexes. Sepharose 92-101 fibronectin 1 Homo sapiens 80-91 3080233-9 1986 These results suggest that human tumor associated macrophages may become tumoricidal under the influence of rIFN-gamma, producing a diffusable substance in agarose culture which causes the observed antiproliferative effects on tumor cells. Sepharose 156-163 interferon gamma Rattus norvegicus 108-118 2422454-0 1986 Affinity chromatography of rat alpha-fetoprotein on estradiol-agarose columns. Sepharose 62-69 alpha-fetoprotein Rattus norvegicus 31-48 2422454-1 1986 The use of 17 beta-estradiol-17-hemisuccinate coupled to agarose beads is shown to be a rapid and simple procedure for the isolation of alpha-fetoprotein (AFP) from amniotic fluid. Sepharose 57-64 alpha-fetoprotein Rattus norvegicus 136-153 2422454-1 1986 The use of 17 beta-estradiol-17-hemisuccinate coupled to agarose beads is shown to be a rapid and simple procedure for the isolation of alpha-fetoprotein (AFP) from amniotic fluid. Sepharose 57-64 alpha-fetoprotein Rattus norvegicus 155-158 3952470-4 1986 By using antihuman C3 antibodies, trypsin treatment, and sodium dodecyl sulphate washing, we were able to demonstrate C3b and iC3b on the agarose beads. Sepharose 138-145 complement C3 Homo sapiens 118-121 3952470-6 1986 We conclude that in vitro human monocytes produce and secrete the essential factors for activation and propagation of the alternative complement pathway (factors C3, B, D, H and I), which becomes evident with an external activator like agarose beads in the cultures. Sepharose 236-243 complement C3 Homo sapiens 162-179 3952470-7 1986 The activation of complement by agarose beads results in the attachment of C3b and iC3b to the surface of the beads, which are then phagocytosed by means of C3b and iC3b receptors on the monocytes. Sepharose 32-39 complement C3 Homo sapiens 75-78 3952470-7 1986 The activation of complement by agarose beads results in the attachment of C3b and iC3b to the surface of the beads, which are then phagocytosed by means of C3b and iC3b receptors on the monocytes. Sepharose 32-39 complement C3 Homo sapiens 84-87 2421435-2 1986 Lysine-binding sites of Lys-plasminogen, heavy chain and fragment K1-3 interact with the guanidyl-carboxyl pair on homoarginine-agarose. Sepharose 128-135 keratin 13 Homo sapiens 66-70 3952793-1 1986 A red cell membrane protein which exhibits Kell blood group antigen activity has been identified with a purified anti-Kell bound to a Protein-A agarose column and eluting with lithium diiodosalicylate (LIS). Sepharose 144-151 Kell metallo-endopeptidase (Kell blood group) Homo sapiens 43-67 3085263-3 1986 The antibodies all inhibit FVIII clotting activity in normal plasma, and when immobilised on agarose retain their ability to recognise and bind the FVIII procoagulant protein. Sepharose 93-100 coagulation factor VIII Homo sapiens 148-153 3486491-0 1986 Improved characterization of plasma von Willebrand factor heterogeneity when using 2.5% agarose gel electrophoresis. Sepharose 88-95 von Willebrand factor Homo sapiens 36-57 3486491-3 1986 By using a 2.5% mixture of two selected agaroses, a single electrophoretic analysis of plasma clearly reveals the extreme complexity of the molecular forms of circulating vWF: each multimeric unit of plasma vWF may be separated into five bands, the central one being predominant. Sepharose 40-48 von Willebrand factor Homo sapiens 171-174 3518132-4 1986 HCII is eluted at 0.28 M NaCl from the heparin-Sepharose column. Sepharose 47-56 serpin family D member 1 Homo sapiens 0-4 3518132-9 1986 Antibodies to HCII were made monospecific by immunoadsorption on HCII-free plasma linked to Sepharose 4B. Sepharose 92-101 serpin family D member 1 Homo sapiens 14-18 3947099-1 1986 Rat liver transketolase (TK) has been purified, in a single step, by immunoaffinity chromatography on specific TK antibodies covalently linked to Sepharose 4B. Sepharose 146-155 transketolase Rattus norvegicus 10-23 3947099-1 1986 Rat liver transketolase (TK) has been purified, in a single step, by immunoaffinity chromatography on specific TK antibodies covalently linked to Sepharose 4B. Sepharose 146-155 transketolase Rattus norvegicus 25-27 2418019-2 1986 The interaction of human apolipoprotein (apo-) E3 with heparin was examined using heparin-Sepharose as a model system. Sepharose 90-99 apolipoprotein E Homo sapiens 25-48 2418024-11 1986 (i) EGF-dependent tyrosine phosphorylation of the EGF receptor and its inhibition by GM3 were also demonstrated on isolated EGF receptor after adsorption on the anti-receptor antibody-Sepharose complex, and the receptor phosphorylation was enhanced on addition of phosphatidylethanolamine. Sepharose 184-193 epidermal growth factor receptor Homo sapiens 50-62 2418024-11 1986 (i) EGF-dependent tyrosine phosphorylation of the EGF receptor and its inhibition by GM3 were also demonstrated on isolated EGF receptor after adsorption on the anti-receptor antibody-Sepharose complex, and the receptor phosphorylation was enhanced on addition of phosphatidylethanolamine. Sepharose 184-193 epidermal growth factor receptor Homo sapiens 124-136 2418113-6 1986 Solubilization of membrane glycoproteins from freshly isolated glomeruli from three human kidneys, in the presence of 0.1% Nonidet P-40, yielded a material that bound to lentil lectin Sepharose and could accelerate the decay of preformed cell-bound amplification C3 convertase sites in a reaction that was inhibited by anti-CR1 antibodies. Sepharose 184-193 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 324-327 3961733-5 1986 When heparin was bound to antithrombin III-Sepharose, the aggregating activity eluted totally in the high-affinity fraction. Sepharose 43-52 serpin family C member 1 Homo sapiens 26-42 3510907-1 1986 The system of translation of Sepharose-bound poly(U) in which all ribosomes are active in peptide elongation was used to determine the stoichiometry of GTP hydrolysis at the stage of EF-Tu-promoted aminoacyl-tRNA binding. Sepharose 29-38 Tu translation elongation factor, mitochondrial Homo sapiens 183-188 3948864-6 1986 On the other hand at pH 6.4 in conditions of physiological ionic strength, fibronectin was retained by both columns, eluting from the DNA-cellulose at 280 mM NaCl and from the heparin-Sepharose column at 210 mM. Sepharose 184-193 fibronectin 1 Homo sapiens 75-86 3948864-7 1986 Furthermore, we have studied the interaction of thermolysin-digested fibronectin both with DNA-cellulose and heparin-Sepharose using the above procedure. Sepharose 117-126 fibronectin 1 Homo sapiens 69-80 3946437-3 1986 Human plasma fibronectin was isolated by gelatin-Sepharose affinity chromatography and evaluated with respect to its opsonic activity following pasteurization, its in vivo clearance kinetics, and its short-term influence on cardiovascular hemodynamics in postoperative septic sheep. Sepharose 49-58 fibronectin 1 Homo sapiens 13-24 2869954-1 1986 By pre-activation with a monoclonal anti-IgM antibody coupled to Sepharose, either in the absence or presence of low doses of lipopolysaccharide, mouse B cell populations were rendered responsive to recombinant DNA derived human interleukin 2 (IL 2). Sepharose 65-74 interleukin 2 Homo sapiens 229-242 2869954-1 1986 By pre-activation with a monoclonal anti-IgM antibody coupled to Sepharose, either in the absence or presence of low doses of lipopolysaccharide, mouse B cell populations were rendered responsive to recombinant DNA derived human interleukin 2 (IL 2). Sepharose 65-74 interleukin 2 Homo sapiens 244-248 3084462-6 1986 The microscale purification system using Con A-Sepharose, PAT-Sepharose, and Hg-agarose column chromatography achieved 565- and 7,970-fold purifications of acid beta-galactosidase with an overall yields of 44% and 45% from normal and GM1-gangliosidosis fibroblasts, respectively. Sepharose 80-87 galactosidase beta 1 Homo sapiens 156-179 2868456-1 1986 Using the under-agarose chemotaxis assay, addition of compounds known to inhibit chemotaxis of leukocytes toward N-f-Met-Leu-Phe (FMLP) was made to the first well in a line of three wells, leukocytes to the middle well, and a slight excess of FMLP to the third well. Sepharose 16-23 formyl peptide receptor 1 Homo sapiens 130-134 3456154-2 1986 The fibrin fragment des-AB N-DSK, which contains the binding sites termed A and B, lost the ability to bind to the site termed a in fibrinogen-Sepharose upon the oxidation of histidine-16 in the B beta chain of fibrinogen [Shimizu, A., Saito, Y., Matsushima, A. Sepharose 143-152 fibrinogen beta chain Homo sapiens 132-142 3456154-2 1986 The fibrin fragment des-AB N-DSK, which contains the binding sites termed A and B, lost the ability to bind to the site termed a in fibrinogen-Sepharose upon the oxidation of histidine-16 in the B beta chain of fibrinogen [Shimizu, A., Saito, Y., Matsushima, A. Sepharose 143-152 fibrinogen beta chain Homo sapiens 211-221 3456154-7 1986 Some of the fragments, which became unable to bind to fibrinogen-Sepharose due to the destruction of site A, however, retained the ability to bind to D-dimer-Sepharose, which contains both sites a and b. Sepharose 65-74 fibrinogen beta chain Homo sapiens 54-64 16664639-1 1986 NADH:nitrate reductase was extracted from corn leaves (Zea mays L. W64A x W182E) and purified on blue Sepharose. Sepharose 102-111 nitrate reductase [NADH] 1 Zea mays 5-22 2867999-3 1986 The tonoplast H+-pumping ATPase from oat roots has been solubilized with Triton X-100 and purified 16-fold by Sepharose 4B chromatography. Sepharose 110-119 dynein axonemal heavy chain 8 Homo sapiens 25-31 3080423-1 1986 A urokinase-type plasminogen activator was purified from conditioned media of several human cell cultures, but preferably from the human lung adenocarcinoma line CALU-3 (ATCC, HTB-55), using a combination of chromatography on zinc chelate-Sepharose, SP-Sephadex C-50, and Sephadex G-100. Sepharose 239-248 plasminogen activator, urokinase Homo sapiens 2-38 3485427-4 1986 Material specifically immunoabsorbed with Sepharose-anti-C1q antibody from a culture medium of cells that was metabolically labelled with [3H] proline or [35S] methionine demonstrated a polypeptide pattern identical with that of serum C1q on SDS/polyacrylamide-gel electrophoresis. Sepharose 42-51 complement C1q A chain Homo sapiens 57-60 3485427-4 1986 Material specifically immunoabsorbed with Sepharose-anti-C1q antibody from a culture medium of cells that was metabolically labelled with [3H] proline or [35S] methionine demonstrated a polypeptide pattern identical with that of serum C1q on SDS/polyacrylamide-gel electrophoresis. Sepharose 42-51 complement C1q A chain Homo sapiens 235-238 3753653-2 1986 8-Aminoguanosine triphosphate, prepared from GTP and hydroxylamine-O-sulfonic acid, was coupled to Sepharose 4B and used as an affinity adsorbent for a 309-fold purification of GTP cyclohydrolase I from human liver. Sepharose 99-111 GTP cyclohydrolase 1 Homo sapiens 177-197 3002036-3 1986 The p52 polypeptides have been purified by chromatography on Blue-, DEAE-, CM-, and Phenyl-Sepharose. Sepharose 90-100 nuclear factor kappa B subunit 2 Homo sapiens 4-7 3079763-4 1986 On agarose gel electrophoresis heart GDH activity was resolved into two fractions (with or without protease inhibitors) while liver had only one fraction. Sepharose 3-10 glutamate dehydrogenase 1 Homo sapiens 37-40 3941087-2 1986 The messenger RNA cap-binding protein (CBP) was isolated from human erythrocyte, rabbit erythrocyte, and rabbit reticulocyte lysate by affinity chromatography on 7-methylguanosine 5"-triphosphate-Sepharose. Sepharose 196-205 eukaryotic translation initiation factor 4E Homo sapiens 18-37 3941087-2 1986 The messenger RNA cap-binding protein (CBP) was isolated from human erythrocyte, rabbit erythrocyte, and rabbit reticulocyte lysate by affinity chromatography on 7-methylguanosine 5"-triphosphate-Sepharose. Sepharose 196-205 eukaryotic translation initiation factor 4E Homo sapiens 39-42 2433908-5 1986 The specificity of the monoclonal antibodies was confirmed by the immunoprecipitation of kininogen with the antibodies coupled to Sepharose-4B followed by SDS-polyacrylamide gel electrophoresis. Sepharose 130-142 kininogen 2-like 1 Rattus norvegicus 89-98 3484918-1 1986 The effect of specific IgG induced by allergy immunotherapy on specific IgE binding in the RAST was assessed by removal of the IgG with staphylococcus protein A bound to Sepharose. Sepharose 170-179 immunoglobulin heavy constant epsilon Homo sapiens 72-75 3004334-3 1986 We have employed the highly purified insulin receptor, immobilized on insulin-Sepharose or eluted in an active form, to define the metal/ATP requirements for kinase activation, the relationship of receptor autophosphorylation to activation, and the kinetic properties of the autophosphorylated, activated receptor kinase. Sepharose 78-87 insulin receptor Homo sapiens 37-53 3518710-3 1986 MIF-like activity was assigned by the ability of various dilutions of ascitic fluids to inhibit migration of guinea pig macrophages from agarose droplets. Sepharose 137-144 macrophage migration inhibitory factor Cavia porcellus 0-3 3518711-3 1986 A macrophage migration inhibition factor (OC-MIF) has been isolated from ascites fluid of ovarian cancer patients by affinity chromatography on L-fucose-Sepharose 6B, and characterized biochemically. Sepharose 153-162 macrophage migration inhibitory factor Homo sapiens 45-48 3492232-0 1986 A simple method to deplete human interleukin 2-containing medium of phytohemagglutinin by filtration through a concanavalin A sepharose 4B column. Sepharose 126-135 interleukin 2 Homo sapiens 33-46 3492232-1 1986 A method is presented to deplete human interleukin 2 (IL-2) containing conditioned medium of phytohemagglutinin (PHA) by filtration through a concanavalin A Sepharose 4B (Con A Seph 4B) column. Sepharose 157-166 interleukin 2 Homo sapiens 39-52 3492232-1 1986 A method is presented to deplete human interleukin 2 (IL-2) containing conditioned medium of phytohemagglutinin (PHA) by filtration through a concanavalin A Sepharose 4B (Con A Seph 4B) column. Sepharose 157-166 interleukin 2 Homo sapiens 54-58 2945695-3 1986 Protein purified by this method comigrates on SDS-PAGE with 125I surface-labeled CR1 isolated by immunoprecipitation or iC3-Sepharose affinity chromatograhy and can be specifically immunoblotted with a second monoclonal anti-CR1 antibody. Sepharose 124-133 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 81-84 2945695-5 1986 Purified E CR1 is functionally active as assessed by its ability to specifically rebind to an iC3-Sepharose affinity column, act as a cofactor for I-mediated cleavage of C3b to C3c and C3d, g and to accelerate decay of both the classical and alternative pathway C3 convertases. Sepharose 98-107 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 11-14 3098490-7 1986 In contrast, direct stimulation of R-MC, NR-MC, or NR-MC plus R monocytes by L4-coated sepharose beads produced lower mean IL2R-50K values of 12 or 13. Sepharose 87-96 interleukin 2 receptor subunit alpha Homo sapiens 123-127 2944742-0 1986 Human acid beta-glucosidase: affinity purification of the normal placental and Gaucher disease splenic enzymes on N-alkyl-deoxynojirimycin-sepharose. Sepharose 139-148 glucosylceramidase beta Homo sapiens 6-27 2944742-1 1986 Two sepharose-bound 1-deoxynojirimycin N-alkyl derivatives, N-(9-carboxynonyl)- and N-(11-carboxyundecyl)-deoxynojirimycin, were used for the affinity purification of acid beta-glucosidase (beta-Glc) from normal and type-1 Ashkenazi Jewish Gaucher disease (AJGD) sources. Sepharose 4-13 glucosylceramidase beta Homo sapiens 167-188 18555370-1 1986 Purified rat liver phenylalanine hydroxylase [L-phenylalanine:tetrahydropteridine:oxygen oxidoreductase (4-hydroxylating), EC 1.14.16.1] was immobilized with activated thiol-Sepharose 4B via disulfide bond formation, which is expected to immobilize the enzyme in its activated form through the SH modification. Sepharose 174-183 phenylalanine hydroxylase Rattus norvegicus 19-44 3091416-1 1986 A rapid and simple method, based on GMP Sepharose affinity chromatography, was used for the purification of human brain hypoxanthine guanine phosphoribosyltransferase. Sepharose 40-49 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 120-166 2435633-11 1986 Multimeric analysis in SDS-agarose gel electrophoresis followed by staining with labelled antifactor VIII antibodies gives information about different polymeric forms of circulating VIII/vW factor. Sepharose 27-34 cytochrome c oxidase subunit 8A Homo sapiens 182-186 2936610-1 1986 Fc gamma receptor-bearing U937 cells, when incubated in serum-free buffer, were found to release spontaneously a suppressor material for pokeweed mitogen-drigen IgG synthesis which could be retained on Sepharose 4B-IgG immunosorbents. Sepharose 202-214 Fc gamma receptor Ia Homo sapiens 0-17 3091420-3 1986 Gel filtration on Sepharose 6B at pH 7.0 revealed two beta-galactosidase forms (GF-1, Mr approximately 500,000-600,000 and GF-2, Mr approximately 190,000-220,000) in reproductive organs and seminal plasma. Sepharose 18-27 GATA binding protein 1 Homo sapiens 54-84 3949058-2 1986 When immobilized on Sepharose the antibodies removed 75% of contaminating proteins in the starting material, including cytochrome P-450. Sepharose 20-29 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 119-135 3001099-11 1986 8G5 IgG-Sepharose columns retained gelsolin (as GCa2) or brevin (as BCa2) in 0.1 mM CaCl2 containing buffers, but released these molecules when eluted with 4 mM EGTA. Sepharose 8-17 gelsolin Homo sapiens 35-43 3721516-1 1986 Phenotyping of plasma plasminogen (PLG) was carried out by the method of agarose gel isoelectric focusing followed by immunofixation or immunoblotting. Sepharose 73-80 plasminogen Homo sapiens 22-33 3721516-1 1986 Phenotyping of plasma plasminogen (PLG) was carried out by the method of agarose gel isoelectric focusing followed by immunofixation or immunoblotting. Sepharose 73-80 plasminogen Homo sapiens 35-38 3721522-0 1986 I (C3b/C4b inactivator) typing by agarose gel isoelectric focusing and immunoblotting technique. Sepharose 34-41 complement factor I Homo sapiens 3-22 3721522-1 1986 Genetic polymorphism of I (C3b/C4b inactivator) was studied by the method of agarose gel isoelectric focusing followed by an immunoblotting technique. Sepharose 77-84 complement factor I Homo sapiens 27-46 3485073-0 1986 Distribution of alpha-1-antitrypsin (Pi) phenotypes in Denmark determined by separator isoelectric focusing in agarose gel. Sepharose 111-118 serpin family A member 1 Homo sapiens 16-35 3485073-1 1986 The distribution of phenotypes of alpha 1-antitrypsin (Pi) in 909 unrelated Danes was determined by the use of separator isoelectric focusing in agarose gel. Sepharose 145-152 serpin family A member 1 Homo sapiens 34-53 3001099-19 1986 Similarly, preformed brevin-actin-Ca2+ complex, equilibrated with EGTA, was retained by 4F8 IgA-Sepharose. Sepharose 96-105 gelsolin Homo sapiens 21-27 3001099-11 1986 8G5 IgG-Sepharose columns retained gelsolin (as GCa2) or brevin (as BCa2) in 0.1 mM CaCl2 containing buffers, but released these molecules when eluted with 4 mM EGTA. Sepharose 8-17 gelsolin Homo sapiens 57-63 3001099-11 1986 8G5 IgG-Sepharose columns retained gelsolin (as GCa2) or brevin (as BCa2) in 0.1 mM CaCl2 containing buffers, but released these molecules when eluted with 4 mM EGTA. Sepharose 8-17 ring finger protein 115 Homo sapiens 68-72 3001099-12 1986 8G5 IgG-Sepharose columns also retained gelsolin-actin-Ca2+ complexes, as GA1Ca2 or higher oligomers from platelet extracts containing 0.1 mM CaCl2. Sepharose 8-17 gelsolin Homo sapiens 40-48 3001099-16 1986 A 4F8 IgA-Sepharose column retained brevin or gelsolin in 0.1 mM CaCl2-containing buffers, but, like the 8G5 IgG, released these molecules when eluted with 4 mM EGTA. Sepharose 10-19 gelsolin Homo sapiens 36-42 3001099-16 1986 A 4F8 IgA-Sepharose column retained brevin or gelsolin in 0.1 mM CaCl2-containing buffers, but, like the 8G5 IgG, released these molecules when eluted with 4 mM EGTA. Sepharose 10-19 gelsolin Homo sapiens 46-54 3940285-5 1986 Treatment of receptors with neuraminidase to remove N-acetylneuraminic acid residues reduced binding to wheat germ agglutinin-agarose by 40%; further treatment with endoglycosidases D and H, to remove all N-linked carbohydrate, decreased binding by a total of 67%. Sepharose 126-133 neuraminidase 1 Bos taurus 28-41 3009990-7 1986 We show that when kidney cytosol is incubated with heparin covalently linked to Sepharose (Sepharose-heparin), after 30 min at 0 degrees C more than 80% of the mineralcorticoid-specific binding sites interact strongly with Sepharose-heparin while CBG is not bound at all. Sepharose 80-89 serpin family A member 6 Rattus norvegicus 247-250 3009990-7 1986 We show that when kidney cytosol is incubated with heparin covalently linked to Sepharose (Sepharose-heparin), after 30 min at 0 degrees C more than 80% of the mineralcorticoid-specific binding sites interact strongly with Sepharose-heparin while CBG is not bound at all. Sepharose 91-100 serpin family A member 6 Rattus norvegicus 247-250 3702404-6 1986 Investigations with induced anti-progesterone receptor antibodies obtained after the fourth immunization show their immunoreactive behaviour towards progesterone receptor in crude cytosol, which was proved by sucrose density gradient centrifugation and by gel filtration on the FPLC system using a Sepharose 12 column. Sepharose 298-307 progesterone receptor Homo sapiens 33-54 3702404-6 1986 Investigations with induced anti-progesterone receptor antibodies obtained after the fourth immunization show their immunoreactive behaviour towards progesterone receptor in crude cytosol, which was proved by sucrose density gradient centrifugation and by gel filtration on the FPLC system using a Sepharose 12 column. Sepharose 298-307 progesterone receptor Homo sapiens 149-170 3009990-7 1986 We show that when kidney cytosol is incubated with heparin covalently linked to Sepharose (Sepharose-heparin), after 30 min at 0 degrees C more than 80% of the mineralcorticoid-specific binding sites interact strongly with Sepharose-heparin while CBG is not bound at all. Sepharose 91-100 serpin family A member 6 Rattus norvegicus 247-250 3009990-11 1986 By application of the newly found property of the mineralcorticoid receptor, an overall 10-fold purified, CBG-free preparation of this receptor can be obtained from kidney cytosol with a single chromatography on Sepharose-heparin. Sepharose 212-221 serpin family A member 6 Rattus norvegicus 106-109 3520164-3 1986 In 4 patients with acute leukemia, a plasma volume of 1500 cm3 was passed in an ex-vivo system over immobilized SpA-Sepharose and then reinfused. Sepharose 116-125 surfactant protein A1 Homo sapiens 112-115 3783420-0 1986 Purification of rat pineal hydroxyindole-O-methyltransferase using S-adenosyl-L-homocysteine agarose chromatography. Sepharose 93-100 acetylserotonin O-methyltransferase Rattus norvegicus 27-60 3783420-1 1986 Rat pineal hydroxyindole-O-methyltransferase (HIOMT; EC 2.1.1.4) was purified by affinity chromatography using an S-adenosyl-L-homocysteine agarose column. Sepharose 140-147 acetylserotonin O-methyltransferase Rattus norvegicus 11-44 3783420-1 1986 Rat pineal hydroxyindole-O-methyltransferase (HIOMT; EC 2.1.1.4) was purified by affinity chromatography using an S-adenosyl-L-homocysteine agarose column. Sepharose 140-147 acetylserotonin O-methyltransferase Rattus norvegicus 46-51 3959599-9 1986 On platelet factor-4 (PF4) Sepharose columns, the HS-PGs from all cells studied bound completely and eluted with considerable heterodispersity. Sepharose 27-36 platelet factor 4 Homo sapiens 22-25 3798003-0 1986 Cells from Xenopus laevis gastrulae adhere to fibronectin-sepharose beads and other lectin coated beads. Sepharose 58-67 fibronectin 1 S homeolog Xenopus laevis 46-57 3905814-8 1985 An antibody against SSB-1 has been prepared in rabbits and purified by SSB-1-Sepharose affinity chromatography. Sepharose 77-86 Hsp70 family ATPase SSB1 Saccharomyces cerevisiae S288C 20-25 3905814-8 1985 An antibody against SSB-1 has been prepared in rabbits and purified by SSB-1-Sepharose affinity chromatography. Sepharose 77-86 Hsp70 family ATPase SSB1 Saccharomyces cerevisiae S288C 71-76 3937276-1 1985 Two variants (I and II) of tissue-type plasminogen activator (t-PA) from human melanoma cells were separated by Lysine Sepharose chromatography. Sepharose 119-128 plasminogen activator, tissue type Homo sapiens 27-60 2415521-6 1985 Thrombin-antithrombin III complex, from either serum or recalcified clotted plasma, bound to vitronectin immobilized on Sepharose or plastic. Sepharose 120-129 coagulation factor II, thrombin Homo sapiens 0-8 2415521-6 1985 Thrombin-antithrombin III complex, from either serum or recalcified clotted plasma, bound to vitronectin immobilized on Sepharose or plastic. Sepharose 120-129 serpin family C member 1 Homo sapiens 9-25 2415521-6 1985 Thrombin-antithrombin III complex, from either serum or recalcified clotted plasma, bound to vitronectin immobilized on Sepharose or plastic. Sepharose 120-129 vitronectin Homo sapiens 93-104 4092689-5 1985 These enzymes could, however, be separated from SF2 by chromatography on heparin-Sepharose. Sepharose 81-90 serine and arginine rich splicing factor 1 Homo sapiens 48-51 3854325-2 1985 Porcine leukocyte elastase was purified from granulocytes by chelating chromatography on copper chelate Sepharose and by ion exchange chromatography on CM-Sepharose. Sepharose 104-113 elastase, neutrophil expressed Homo sapiens 8-26 3854325-2 1985 Porcine leukocyte elastase was purified from granulocytes by chelating chromatography on copper chelate Sepharose and by ion exchange chromatography on CM-Sepharose. Sepharose 155-164 elastase, neutrophil expressed Homo sapiens 8-26 4093448-1 1985 Staphylocoagulase with a molecular weight of 64,000 and subspecies ranging in molecular weight from 36,000 to 64,000 were purified by affinity column chromatography on bovine prothrombin-Sepharose 4B from the culture filtrates of the Staphylococcus aureus strains, st-213 and 104. Sepharose 187-196 coagulation factor II, thrombin Homo sapiens 175-186 2415639-2 1985 A slow decrease in pH was shown to be more efficient in eluting human alpha-fetoprotein bound to Sepharose-immobilized antibodies than a pH shock. Sepharose 97-106 alpha fetoprotein Homo sapiens 70-87 2416314-2 1985 Calmodulin containing HuIFN-alpha was retained on a trifluorophenothiazine-Sepharose column. Sepharose 75-84 calmodulin 1 Homo sapiens 0-10 3904001-3 1985 Preparations enriched in the insulin receptor and calmodulin-binding proteins were isolated from detergent-solubilized rat adipocyte membranes by chromatography with wheat germ agglutinin agarose and calmodulin-conjugated Sepharose, respectively. Sepharose 188-195 insulin receptor Rattus norvegicus 29-45 3904001-5 1985 Binding and photocovalent cross-linking of iodine-125-labeled calmodulin to these affinity-purified preparations and to isolated plasma membranes, followed by immunoadsorption with insulin receptor antibodies bound to protein A Sepharose, resulted in significant purification of a binding complex of 110K to 140K. Sepharose 228-237 insulin receptor Rattus norvegicus 181-197 4074346-3 1985 Insulin receptors in Triton X-100-solubilized microsomal membranes were purified 2,000-fold by sequential affinity chromatography on wheat germ lectin-agarose and insulin-CDI-activated agarose. Sepharose 151-158 insulin Homo sapiens 0-7 4074346-3 1985 Insulin receptors in Triton X-100-solubilized microsomal membranes were purified 2,000-fold by sequential affinity chromatography on wheat germ lectin-agarose and insulin-CDI-activated agarose. Sepharose 185-192 insulin Homo sapiens 0-7 4074346-3 1985 Insulin receptors in Triton X-100-solubilized microsomal membranes were purified 2,000-fold by sequential affinity chromatography on wheat germ lectin-agarose and insulin-CDI-activated agarose. Sepharose 185-192 insulin Homo sapiens 163-170 4084546-2 1985 An Mr 19 000 protein (destrin) that has the ability to rapidly depolymerize F-actin in a stoichiometric manner was purified from porcine kidney by sequential chromatography on DNase I-agarose, hydroxyapatite, and Sephadex G-75. Sepharose 184-191 destrin, actin depolymerizing factor Homo sapiens 22-29 4042983-10 1985 Most of the oligosaccharides from dissociated hCG alpha bound to ConA-Sepharose (72%), while less material from free alpha (40%) and even less from free alpha 2 (25%) were capable of binding to ConA. Sepharose 70-79 chromogranin A Homo sapiens 46-55 4043557-2 1985 In the present study, we found a subpopulation of antibodies (eluted from a protein A-Sepharose affinity column at pH 4.3) directed at the human fibroblast insulin receptor. Sepharose 86-95 insulin receptor Homo sapiens 156-172 4088429-1 1985 Cathepsin B has been purified 750-fold to apparent homogeneity from human and bovine brain cortex using ammonium sulfate fractionation (30-70%), chromatography on Sephadex G-100, CM-Sephadex C-50, and concanavalin A-Sepharose. Sepharose 216-225 cathepsin B Homo sapiens 0-11 4071686-2 1985 A comparison of properties of soluble and membrane brain amino-peptidases carried out by means of micro-scale phenyl-sepharose and ConA-sepharose column chromatography shows that the membrane-bound aminopeptidase is a glycoprotein which possesses a high capacity to hydrophobic interactions. Sepharose 117-126 carboxypeptidase Q Homo sapiens 198-212 3511088-4 1986 Hybridoma supernatants exhibiting dual reactivity were absorbed over HIgG and bovine serum albumin (BSA)-Sepharose immunoabsorbent columns. Sepharose 105-114 albumin Homo sapiens 85-98 24442296-1 1986 Cytochrome bc 1 complexes have been isolated from wild type Rhodopseudomonas viridis and Rhodospirillum rubrum and purified by affinity chromatography on cytochrome c-Sepharose 4B. Sepharose 167-176 cytochrome c, somatic Equus caballus 154-166 3079907-7 1986 As with pp35, EGF(Uro)-stimulated phosphorylation of isolated rabbit liver beta-35 was observed in a reconstituted system using either EDTA/EGTA-washed placental membranes or solubilized EGF(Uro) receptor immobilized on concanavalin A-agarose. Sepharose 235-242 epidermal growth factor Homo sapiens 14-22 3092207-2 1986 The IFN was purified by a three-step procedure using controlled pore glass adsorption chromatography, concanavalin A (ConA)-agarose column chromatography and gel filtration. Sepharose 124-131 interferon alpha 1 Homo sapiens 4-7 2428091-3 1986 AFP was isolated from Nya:NYLAR mouse amniotic fluid (MAF) by polyacrylamide gel electrophoresis plus Blue Sepharose affinity chromatography (PAGE/BS), and by affinity chromatographies employing immobilized E2 and rabbit anti-AFP. Sepharose 107-116 alpha fetoprotein Mus musculus 0-3 3088865-0 1986 [Microprecipitation in agarose gel as a detection method for C-reactive protein in human serum]. Sepharose 23-30 C-reactive protein Homo sapiens 61-79 3776354-1 1986 The distribution of the human red cell esterase D (EsD) "extended" polymorphism in a population sample from Tuscany (Italy) was studied using agarose gel isoelectric focusing. Sepharose 142-149 esterase D Homo sapiens 39-49 3776354-1 1986 The distribution of the human red cell esterase D (EsD) "extended" polymorphism in a population sample from Tuscany (Italy) was studied using agarose gel isoelectric focusing. Sepharose 142-149 esterase D Homo sapiens 51-54 4074740-0 1985 Characterization of plasma membrane domains of mouse EL4 lymphoma cells obtained by affinity chromatography on concanavalin A-Sepharose. Sepharose 126-135 epilepsy 4 Mus musculus 53-56 4078307-8 1985 A more direct demonstration of chemotaxis was achieved by the second assay in which an agarose slab containing FMLP was incorporated into a gel, and the paths of nearby neutrophils were filmed. Sepharose 87-94 formyl peptide receptor 1 Homo sapiens 111-115 2999099-2 1985 Angiotensin-converting enzyme was purified from human lung, kidney, testis, blood plasma, and seminal plasma using a facile two-step protocol which included affinity chromatography on Sepharose-bound lisinopril followed by either gel filtration or hydroxylapatite chromatography. Sepharose 184-193 angiotensin I converting enzyme Homo sapiens 0-29 4083898-9 1985 The monoclonal IgG was covalently linked to a Sepharose 4B column and was used for immunoaffinity chromatography of cytochrome P-450AROM. Sepharose 46-55 cytochrome P450 family 19 subfamily A member 1 Homo sapiens 116-136 3877533-8 1985 Analysis of vWF multimeric structure by agarose gel electrophoresis, including a newly developed high-resolution technique, demonstrated that the main band of each multimer was present, but a second, well-defined band always seen in normal individuals was missing in the patients. Sepharose 40-47 von Willebrand factor Homo sapiens 12-15 3935177-1 1985 Two molecular variants, of thyroxin-binding globulin (TBG), TBG-1 and TBG-2, were obtained from human retroplacental blood by fractionation of pure TBG on concanavalin A-Sepharose. Sepharose 170-179 serpin family A member 7 Homo sapiens 27-52 3935177-1 1985 Two molecular variants, of thyroxin-binding globulin (TBG), TBG-1 and TBG-2, were obtained from human retroplacental blood by fractionation of pure TBG on concanavalin A-Sepharose. Sepharose 170-179 serpin family A member 7 Homo sapiens 54-57 4084323-2 1985 A simple and reproducible purification procedure of homogeneous DNA polymerase beta from rat liver is developed, including sedimentation and saline extraction of rat liver chromatin, chromatography of the extract on DEAE-cellulose, phosphocellulose, Gel Blue A, and DNA sepharose. Sepharose 270-279 DNA polymerase beta Rattus norvegicus 64-83 3005246-2 1985 The purification employed calmodulin-Sepharose 4B affinity chromatography; elution was performed with a free Ca2+ gradient. Sepharose 37-46 calmodulin 1 Rattus norvegicus 26-36 2933458-4 1985 In addition, Fab KuFc79-conjugated Sepharose was used to isolate functional IgG Fc-binding molecules from soluble extracts of the human monocyte-like cell line U937. Sepharose 35-44 FA complementation group B Homo sapiens 13-16 2999440-1 1985 Human cytomegalovirus-induced DNA polymerase and cellular DNA polymerase alpha were purified by successive chromatography on DEAE-cellulose, phosphocellulose, heparin agarose, and single-stranded DNA agarose columns. Sepharose 167-174 DNA polymerase alpha 1, catalytic subunit Homo sapiens 58-78 2999440-1 1985 Human cytomegalovirus-induced DNA polymerase and cellular DNA polymerase alpha were purified by successive chromatography on DEAE-cellulose, phosphocellulose, heparin agarose, and single-stranded DNA agarose columns. Sepharose 200-207 DNA polymerase alpha 1, catalytic subunit Homo sapiens 58-78 3003212-4 1985 The other is an improved RIA using a specific antiserum to as beta-CTP, in which extraction of hCG with monoclonal antibody-Sepharose 4B conjugate is used to obtain increased sensitivity with retention of specificity. Sepharose 124-136 chorionic gonadotropin subunit beta 5 Homo sapiens 95-98 3909150-3 1985 The bacterially produced apoE was purified by heparin-Sepharose affinity chromatography, Sephacryl S-300 gel filtration, and preparative Immobiline isoelectric focusing. Sepharose 54-63 apolipoprotein E Homo sapiens 25-29 3905970-3 1985 By this method we have demonstrated the existence of multiple molecular forms of the complement factors C3 and factor B in serum from 2 species, man and chicken, after electrophoretic separation in agarose. Sepharose 198-205 complement C3 Homo sapiens 104-119 4074367-0 1985 Rapid purification of calmodulin and S-100 protein by affinity chromatography with melittin immobilized to sepharose. Sepharose 107-116 calmodulin Bos taurus 22-32 4074367-1 1985 Melittin-Sepharose was prepared for Ca2+-dependent affinity chromatography of calmodulin and S-100 protein. Sepharose 9-18 calmodulin Bos taurus 78-88 4074367-3 1985 Recovery of calmodulin from melittin-Sepharose was related to the degree of saturation of column capacity with lower yields when only partial saturation was achieved. Sepharose 37-46 calmodulin Bos taurus 12-22 4074736-0 1985 Human alpha-thrombin binding to nonpolymerized fibrin-Sepharose: evidence for an anionic binding region. Sepharose 54-63 coagulation factor II, thrombin Homo sapiens 12-20 2997330-4 1985 The chemotactic tripeptide FMLP was incorporated into 1% agarose containing 0.1% bovine serum albumin (BSA) to give a concentration range of 10(-8) M to 10(-6) M FMLP. Sepharose 57-64 formyl peptide receptor 1 Homo sapiens 27-31 3002438-7 1985 The human c-myc protein was enriched about 3000-fold from Colo320 cells using c-myc-specific IgG coupled to Sepharose beads. Sepharose 108-117 MYC proto-oncogene, bHLH transcription factor Homo sapiens 10-15 4091264-0 1985 Separation of fodrin subunits by affinity chromatography on calmodulin-Sepharose. Sepharose 71-80 calmodulin Bos taurus 60-70 4091264-3 1985 This has been exploited for separating the fodrin subunits rapidly and quantitatively by affinity chromatography on calmodulin-Sepharose. Sepharose 127-136 calmodulin Bos taurus 116-126 3932582-3 1985 A monoclonal anti-BSF-1 antibody coupled to Sepharose depleted both BSF-1 and BCDF-gamma activity but not BCDF-mu activity from two different T cell supernatants. Sepharose 44-53 interleukin 4 Homo sapiens 18-23 3932582-3 1985 A monoclonal anti-BSF-1 antibody coupled to Sepharose depleted both BSF-1 and BCDF-gamma activity but not BCDF-mu activity from two different T cell supernatants. Sepharose 44-53 interleukin 4 Homo sapiens 68-73 3934016-2 1985 Glycosylated, processed forms of angiotensinogen were isolated by chromatography on lentil lectin-Sepharose 4B. Sepharose 98-107 angiotensinogen Homo sapiens 33-48 3910088-1 1985 A 24 000-dalton protein [yeast eukaryotic initiation factor 4E (eIF-4E)] was purified from yeast Saccharomyces cerevisiae postribosomal supernatant by m7GDP-agarose affinity chromatography. Sepharose 157-164 eukaryotic translation initiation factor 4E Homo sapiens 64-70 3910088-2 1985 The protein behaves very similarly to mammalian protein synthesis initiation factor eIF-4E with respect to binding to and elution from m7GDP-agarose columns and cross-linking to oxidized reovirus mRNA cap structures. Sepharose 141-148 eukaryotic translation initiation factor 4E Homo sapiens 84-90 4062902-1 1985 S-protein, the main inhibitor of the assembly of the membrane attack complex of complement, was isolated from human plasma by a simple purification procedure, which includes barium citrate adsorption, ammonium sulphate precipitation, chromatography on DEAE-Sephacel and Blue Sepharose and gel filtration on Sephacryl S-200. Sepharose 275-284 vitronectin Homo sapiens 0-9 3930491-2 1985 Human platelet thrombospondin (TSP) was purified to homogeneity by chromatography on fibrinogen coupled to cyanogen bromide-activated Sepharose. Sepharose 134-143 thrombospondin 1 Homo sapiens 15-29 3840166-2 1985 The binding constant of estrogen receptor-DNA interaction was determined by analysis of the exponential elution profile of the estrogen receptor from DNA-Sepharose columns using Tris buffer at a constant salt concentration. Sepharose 154-163 estrogen receptor 1 Homo sapiens 24-41 3840166-2 1985 The binding constant of estrogen receptor-DNA interaction was determined by analysis of the exponential elution profile of the estrogen receptor from DNA-Sepharose columns using Tris buffer at a constant salt concentration. Sepharose 154-163 estrogen receptor 1 Homo sapiens 127-144 3930491-2 1985 Human platelet thrombospondin (TSP) was purified to homogeneity by chromatography on fibrinogen coupled to cyanogen bromide-activated Sepharose. Sepharose 134-143 thrombospondin 1 Homo sapiens 31-34 3930491-10 1985 TSP binding to fibrinogen-Sepharose occurred in the presence of EDTA, indicating that calcium and magnesium ions are not required for interaction of TSP with fibrinogen. Sepharose 26-35 thrombospondin 1 Homo sapiens 0-3 3930491-10 1985 TSP binding to fibrinogen-Sepharose occurred in the presence of EDTA, indicating that calcium and magnesium ions are not required for interaction of TSP with fibrinogen. Sepharose 26-35 fibrinogen beta chain Homo sapiens 15-25 3930491-11 1985 The binding of TSP to fibrinogen-Sepharose was quantitatively blocked by pretreatment with an antibody to the cyanogen bromide cleavage fragment composed of residues 241-476 of the carboxyl-terminal end of the alpha chain of fibrinogen. Sepharose 33-42 thrombospondin 1 Homo sapiens 15-18 3930491-11 1985 The binding of TSP to fibrinogen-Sepharose was quantitatively blocked by pretreatment with an antibody to the cyanogen bromide cleavage fragment composed of residues 241-476 of the carboxyl-terminal end of the alpha chain of fibrinogen. Sepharose 33-42 fibrinogen beta chain Homo sapiens 22-32 3930491-11 1985 The binding of TSP to fibrinogen-Sepharose was quantitatively blocked by pretreatment with an antibody to the cyanogen bromide cleavage fragment composed of residues 241-476 of the carboxyl-terminal end of the alpha chain of fibrinogen. Sepharose 33-42 fibrinogen beta chain Homo sapiens 225-235 3930491-13 1985 Excess fibrinogen (30 mg/ml) added to platelet extract quantitatively inhibited binding of TSP to fibrinogen-Sepharose. Sepharose 109-118 fibrinogen beta chain Homo sapiens 7-17 3930491-13 1985 Excess fibrinogen (30 mg/ml) added to platelet extract quantitatively inhibited binding of TSP to fibrinogen-Sepharose. Sepharose 109-118 thrombospondin 1 Homo sapiens 91-94 3930491-13 1985 Excess fibrinogen (30 mg/ml) added to platelet extract quantitatively inhibited binding of TSP to fibrinogen-Sepharose. Sepharose 109-118 fibrinogen beta chain Homo sapiens 98-108 3929829-2 1985 In addition, SDS-agarose gel electrophoresis demonstrated alterations of the von Willebrand factor (vWF) multimeric structure. Sepharose 17-24 von Willebrand factor Homo sapiens 77-98 4041471-10 1985 Thus, avian adipose lipoprotein lipase has been purified by a one-step immunoaffinity followed by a concentrating step on heparin-Sepharose 4B. Sepharose 130-139 lipoprotein lipase Mus musculus 20-38 2413806-2 1985 Purification of tyrosyl-phosphorylated insulin receptor was effected by adsorption on and elution (with a hapten) from a column of O-phosphotyrosyl-binding antibody immobilized on protein A-Sepharose (Ab-protein A). Sepharose 190-199 insulin receptor Homo sapiens 39-55 4062888-3 1985 Complement receptor type 3(CR3) was eluted from iC3b-Sepharose by removal of bivalent cations. Sepharose 53-62 teratocarcinoma-derived growth factor 1 pseudogene 3 Homo sapiens 0-30 4062888-7 1985 Preabsorption with iC3b-Sepharose before the anti-(CR3 beta-subunit) antibody column showed that iC3b binds CR3 and p150,95, the smallest member of the group of three homologous proteins that share the same beta-subunit. Sepharose 24-33 teratocarcinoma-derived growth factor 1 pseudogene 3 Homo sapiens 108-111 4062888-7 1985 Preabsorption with iC3b-Sepharose before the anti-(CR3 beta-subunit) antibody column showed that iC3b binds CR3 and p150,95, the smallest member of the group of three homologous proteins that share the same beta-subunit. Sepharose 24-33 chromatin assembly factor 1 subunit A Homo sapiens 116-120 4028522-2 1985 The activated form of the third component of complement, C3b, which had bound to the radioactive cysteine was then released from the agarose bead with dithiothreitol. Sepharose 133-140 endogenous retrovirus group K member 3 Homo sapiens 57-60 3002775-3 1985 Preferential AFB1 bindings were identified in both PLC and hHC DNAs compared to normal liver DNA when analyzed by restriction endonuclease digestions and agarose gel electrophoresis. Sepharose 154-161 calcium sensing receptor Homo sapiens 59-62 4043081-3 1985 Human alpha-L-iduronidase from liver was purified about 20 000-fold with a new rapid three-step, five-column procedure which consisted of a Concanavalin-A-Sepharose/Blue-A-Agarose coupled step, a CM-Sepharose/Bio-Gel HT coupled step followed by a cupric-ion-chelating Sepharose 6B step. Sepharose 155-164 alpha-L-iduronidase Homo sapiens 6-25 4043081-3 1985 Human alpha-L-iduronidase from liver was purified about 20 000-fold with a new rapid three-step, five-column procedure which consisted of a Concanavalin-A-Sepharose/Blue-A-Agarose coupled step, a CM-Sepharose/Bio-Gel HT coupled step followed by a cupric-ion-chelating Sepharose 6B step. Sepharose 172-179 alpha-L-iduronidase Homo sapiens 6-25 4043081-3 1985 Human alpha-L-iduronidase from liver was purified about 20 000-fold with a new rapid three-step, five-column procedure which consisted of a Concanavalin-A-Sepharose/Blue-A-Agarose coupled step, a CM-Sepharose/Bio-Gel HT coupled step followed by a cupric-ion-chelating Sepharose 6B step. Sepharose 199-208 alpha-L-iduronidase Homo sapiens 6-25 4043081-3 1985 Human alpha-L-iduronidase from liver was purified about 20 000-fold with a new rapid three-step, five-column procedure which consisted of a Concanavalin-A-Sepharose/Blue-A-Agarose coupled step, a CM-Sepharose/Bio-Gel HT coupled step followed by a cupric-ion-chelating Sepharose 6B step. Sepharose 199-208 alpha-L-iduronidase Homo sapiens 6-25 4043085-3 1985 Glycopeptides liberated from apolipoprotein-B by pronase were fractionated by affinity chromatography on concanavalin-A--Sepharose. Sepharose 121-130 apolipoprotein B Homo sapiens 29-45 2867060-1 1985 Rabbit liver arylsulfatase A (arylsulfatase sulfohydrolase, EC 3.1.6.1) monomer was immobilized on cyanogen bromide-activated Sepharose-6MB and on Affi-Gel-10 under various experimental conditions in order to study the effects of variables in sulfatase monomer/oligomer subunit affinity chromatography. Sepharose 126-135 arylsulfatase A Homo sapiens 13-28 2867060-1 1985 Rabbit liver arylsulfatase A (arylsulfatase sulfohydrolase, EC 3.1.6.1) monomer was immobilized on cyanogen bromide-activated Sepharose-6MB and on Affi-Gel-10 under various experimental conditions in order to study the effects of variables in sulfatase monomer/oligomer subunit affinity chromatography. Sepharose 126-135 arylsulfatase family member H Homo sapiens 17-26 2415667-1 1985 Agarose-bound asialofetuin functions as an insoluble sialyl group acceptor in a simplified assay for sialyl transferase (CMP-neuraminate: D-galactosyl-glycoprotein N-acetylneuraminyl transferase; EC 2.4.99.1) in serum. Sepharose 0-7 ST6 beta-galactoside alpha-2,6-sialyltransferase 2 Homo sapiens 101-119 2932472-2 1985 Plasma ANF was extracted before radioimmunoassay by affinity chromatography on a column of ANF antibody-coupled agarose. Sepharose 112-119 natriuretic peptide A Homo sapiens 7-10 2932472-2 1985 Plasma ANF was extracted before radioimmunoassay by affinity chromatography on a column of ANF antibody-coupled agarose. Sepharose 112-119 natriuretic peptide A Homo sapiens 91-94 2933484-0 1985 An agarose gel electrophoretic method for typing phosphoglucomutase-1, esterase D, or glyoxalase I. Sepharose 3-10 phosphoglucomutase 1 Homo sapiens 49-69 2933484-0 1985 An agarose gel electrophoretic method for typing phosphoglucomutase-1, esterase D, or glyoxalase I. Sepharose 3-10 esterase D Homo sapiens 71-81 2933484-0 1985 An agarose gel electrophoretic method for typing phosphoglucomutase-1, esterase D, or glyoxalase I. Sepharose 3-10 glyoxalase I Homo sapiens 86-98 2933484-1 1985 A conventional agarose gel electrophoretic method was described for typing phosphoglucomutase-1, esterase D, or glyoxalase I as single systems. Sepharose 15-22 phosphoglucomutase 1 Homo sapiens 75-95 2933484-1 1985 A conventional agarose gel electrophoretic method was described for typing phosphoglucomutase-1, esterase D, or glyoxalase I as single systems. Sepharose 15-22 esterase D Homo sapiens 97-107 2933484-1 1985 A conventional agarose gel electrophoretic method was described for typing phosphoglucomutase-1, esterase D, or glyoxalase I as single systems. Sepharose 15-22 glyoxalase I Homo sapiens 112-124 3161948-2 1985 Tryptase (5 micrograms/ml) inactivated the thrombin-induced clotting activity of fibrinogen (100 micrograms/ml) with essentially similar t 1/2 values of 4.6 min in the absence of heparin and 5.8 min in the presence of heparin (20 micrograms/ml) that were not appreciably different than with lysine-Sepharose-purified plasmin (5 micrograms/ml). Sepharose 298-307 coagulation factor II, thrombin Homo sapiens 43-51 3876401-5 1985 IL-1 activity from lipopolysaccharide-treated endothelial cell supernatants could be absorbed by an antibody to IL-1 coupled to Sepharose. Sepharose 128-137 interleukin 1 alpha Homo sapiens 0-4 3876401-5 1985 IL-1 activity from lipopolysaccharide-treated endothelial cell supernatants could be absorbed by an antibody to IL-1 coupled to Sepharose. Sepharose 128-137 interleukin 1 alpha Homo sapiens 112-116 3928743-8 1985 Anti-Tp67 and anti-Tp44 were able to sustain continuous proliferation of anti-CD3-Sepharose-stimulated T cells for up to 2.5 wk without exogenous IL 2 or feeder cells. Sepharose 82-91 CD28 molecule Homo sapiens 19-23 3928753-0 1985 Recombinant interferon-gamma inhibits the B cell proliferative response stimulated by soluble but not by Sepharose-bound anti-immunoglobulin antibody. Sepharose 105-114 interferon gamma Homo sapiens 12-28 3863119-1 1985 A factor with tumor necrosis factor (TNF) activity produced by the LuKII human lymphoblastoid cell line [designated TNF(LuKII)] was purified sequentially by using controlled-pore glass, lentil lectin-Sepharose, and procion red agarose chromatography, yielding TNF with a specific activity of 1.5 X 10(7) units per mg of protein and an isoelectric point of approximately equal to 6.7. Sepharose 200-209 tumor necrosis factor Homo sapiens 37-40 2414278-1 1985 We demonstrate by agarose gel electrophoresis and DNase I footprinting that Xenopus transcription factor A promotes DNA reassociation. Sepharose 18-25 general transcription factor 3A L homeolog Xenopus laevis 98-106 2411431-12 1985 Inability of PAF to bind Cibacron Blue-Sepharose, unlike IFN, supports this notion. Sepharose 39-48 patchy fur Mus musculus 13-16 2413452-1 1985 We have isolated and studied two alloreactive, T4+, human lymphocyte clones that release interleukin 2 (IL-2) and interleukin 3 (IL-3) bioactivities upon stimulation with IL-2, alloantigen, or Sepharose-conjugated antibodies directed against the T3 protein. Sepharose 193-202 interleukin 2 Homo sapiens 89-102 2413452-1 1985 We have isolated and studied two alloreactive, T4+, human lymphocyte clones that release interleukin 2 (IL-2) and interleukin 3 (IL-3) bioactivities upon stimulation with IL-2, alloantigen, or Sepharose-conjugated antibodies directed against the T3 protein. Sepharose 193-202 interleukin 2 Homo sapiens 104-108 2413452-1 1985 We have isolated and studied two alloreactive, T4+, human lymphocyte clones that release interleukin 2 (IL-2) and interleukin 3 (IL-3) bioactivities upon stimulation with IL-2, alloantigen, or Sepharose-conjugated antibodies directed against the T3 protein. Sepharose 193-202 interleukin 3 Homo sapiens 114-133 3840916-2 1985 HC II was functionally determined by thrombin inhibition in the presence of heparin in AT III-free plasma prepared by immunoadsorption on anti-AT III-Sepharose 4B column. Sepharose 150-159 serpin family D member 1 Homo sapiens 0-5 3862666-6 1985 Phosphorylation by casein kinase I alters binding of the aminoacyl-tRNA synthetase complex to tRNA-Sepharose. Sepharose 99-108 casein kinase I Oryctolagus cuniculus 19-34 3900060-2 1985 Endothelial cell growth factor (ECGF) can be rapidly purified from bovine brain to high specific activity using heparin-Sepharose affinity chromatography. Sepharose 120-129 fibroblast growth factor 1 Bos taurus 0-30 3900060-2 1985 Endothelial cell growth factor (ECGF) can be rapidly purified from bovine brain to high specific activity using heparin-Sepharose affinity chromatography. Sepharose 120-129 fibroblast growth factor 1 Bos taurus 32-36 3900060-5 1985 The two forms of ECGF can be separated by either NaCl gradient elution from heparin-Sepharose or reversed-phase high pressure liquid chromatography. Sepharose 84-93 fibroblast growth factor 1 Bos taurus 17-21 3929829-2 1985 In addition, SDS-agarose gel electrophoresis demonstrated alterations of the von Willebrand factor (vWF) multimeric structure. Sepharose 17-24 von Willebrand factor Homo sapiens 100-103 3907813-1 1985 In previous work, we identified two insulin receptor species, RI (KAV = 0.31) and RII (KAV = 0.53), that could be separated by gel filtration on Sepharose 6B. Sepharose 145-154 insulin receptor Homo sapiens 36-52 4085662-8 1985 The anionic GST-corticosterone complex bound to DNA coupled Sepharose at 25 degrees C and passed through the column at 4 degrees C. Conversely, the complex bound to a DEAE cellulose column at 4 degrees C but passed through at 25 degrees C. These properties of anionic GST are quite similar to those of glucocorticoid receptor of rat liver cytosol reported previously. Sepharose 60-69 hematopoietic prostaglandin D synthase Rattus norvegicus 12-15 2411730-10 1985 Sepharose CL-6B chromatography of RBL-1 proteoglycans digested with chondroitinase ABC revealed that less than 7% of the molecules in the digest chromatographed with the hydrodynamic size of undigested proteoglycans, suggesting that at most 7% of the proteoglycans lack chondroitin sulfate glycosaminoglycans. Sepharose 0-9 RB transcriptional corepressor like 1 Rattus norvegicus 34-39 3929855-3 1985 Upon passing modified LDL through a fibronectin-collagen-Sepharose column the desorption of fibronectin occurred. Sepharose 57-66 fibronectin 1 Homo sapiens 92-103 2411394-4 1985 Passage of the unagglutinated radiolabeled cells through SBA-Sepharose columns results in further purging of 28-53% of the neuroblastoma cells. Sepharose 61-70 lectin Glycine max 57-60 2411394-6 1985 It seems therefore that the agglutination technique, or the use of SBA-Sepharose columns, can be used only as a preliminary step for the purging of neuroblastoma cells from involved human bone marrow preparations. Sepharose 71-80 lectin Glycine max 67-70 2411735-4 1985 In the present study, a solid-phase separation assay verified the specific formation of a ternary complex of 192-IgG, the NGF receptor, and NGF: 125I-labeled 192-IgG precipitated from solution only when incubated with both solubilized NGF receptor and NGF covalently linked to a solid phase (Sepharose 4B). Sepharose 292-301 nerve growth factor receptor Rattus norvegicus 122-134 3876600-0 1985 Fibronectin binds to complement-coated agarose beads and increases their association to mouse macrophages. Sepharose 39-46 fibronectin 1 Mus musculus 0-11 3876600-1 1985 We have studied the binding of fibronectin to complement (C3b, C3bi, C3d)-coated agarose beads and its effect on cell association of such beads to mouse macrophages. Sepharose 81-88 fibronectin 1 Mus musculus 31-42 3876600-2 1985 Fibronectin bound to agarose beads preincubated in human serum, whereas no binding occurred after preincubation of the beads with complement-inactivated (50 degrees C for 20 min or ethylenediaminetetraacetic acid) sera. Sepharose 21-28 fibronectin 1 Mus musculus 0-11 3876600-5 1985 A similar amount of fibronectin bound to agarose beads coated with equimolar amounts of C3b, C3bi, or C3d, suggesting that the common domain C3d carries the main binding site(s) for fibronectin. Sepharose 41-48 fibronectin 1 Mus musculus 20-31 3876600-6 1985 Preincubation of serum-treated and trypsinized agarose beads with fibronectin led to an increased association (22%) of such beads to mouse macrophages. Sepharose 47-54 fibronectin 1 Mus musculus 66-77 3876600-7 1985 The results indicate that fibronectin promotes binding of complement-coated agarose beads to mouse macrophages, whereas the ingestion of the beads is mediated via complement C3 receptors. Sepharose 76-83 fibronectin 1 Mus musculus 26-37 2994662-1 1985 The EGF receptor has been purified from human epidermoid carcinoma A431 cells by affinity chromatography on wheat germ agglutinin-agarose and tyrosine-Sepharose. Sepharose 130-137 epidermal growth factor receptor Homo sapiens 4-16 2994662-1 1985 The EGF receptor has been purified from human epidermoid carcinoma A431 cells by affinity chromatography on wheat germ agglutinin-agarose and tyrosine-Sepharose. Sepharose 151-160 epidermal growth factor receptor Homo sapiens 4-16 2994666-5 1985 The oncomodulin concentration required for the half-maximal activation of the heart phosphodiesterase was estimated to be 2 X 10(-7)M. In addition, the possibility of the observed activation by oncomodulin being due to calmodulin contamination can be ruled out as the oncomodulin activation profiles were unaltered subsequent to chromatography on organomercurial agarose and the activation by oncomodulin could not be reversed by anti-calmodulin IgG. Sepharose 363-370 LOC100301381 Bos taurus 4-15 2994666-5 1985 The oncomodulin concentration required for the half-maximal activation of the heart phosphodiesterase was estimated to be 2 X 10(-7)M. In addition, the possibility of the observed activation by oncomodulin being due to calmodulin contamination can be ruled out as the oncomodulin activation profiles were unaltered subsequent to chromatography on organomercurial agarose and the activation by oncomodulin could not be reversed by anti-calmodulin IgG. Sepharose 363-370 LOC100301381 Bos taurus 194-205 2994666-5 1985 The oncomodulin concentration required for the half-maximal activation of the heart phosphodiesterase was estimated to be 2 X 10(-7)M. In addition, the possibility of the observed activation by oncomodulin being due to calmodulin contamination can be ruled out as the oncomodulin activation profiles were unaltered subsequent to chromatography on organomercurial agarose and the activation by oncomodulin could not be reversed by anti-calmodulin IgG. Sepharose 363-370 calmodulin Bos taurus 219-229 2994666-5 1985 The oncomodulin concentration required for the half-maximal activation of the heart phosphodiesterase was estimated to be 2 X 10(-7)M. In addition, the possibility of the observed activation by oncomodulin being due to calmodulin contamination can be ruled out as the oncomodulin activation profiles were unaltered subsequent to chromatography on organomercurial agarose and the activation by oncomodulin could not be reversed by anti-calmodulin IgG. Sepharose 363-370 LOC100301381 Bos taurus 194-205 2994666-5 1985 The oncomodulin concentration required for the half-maximal activation of the heart phosphodiesterase was estimated to be 2 X 10(-7)M. In addition, the possibility of the observed activation by oncomodulin being due to calmodulin contamination can be ruled out as the oncomodulin activation profiles were unaltered subsequent to chromatography on organomercurial agarose and the activation by oncomodulin could not be reversed by anti-calmodulin IgG. Sepharose 363-370 LOC100301381 Bos taurus 194-205 2991266-3 1985 By affinity chromatography of membrane proteins on 2-OH-3,5-diiodobenzoic acid-Sepharose and gel filtration on Ultrogel a fraction enriched in auxin-binding protein (ABP) was obtained and used for rabbit immunization. Sepharose 79-88 sex hormone-binding globulin Oryctolagus cuniculus 166-169 2991266-5 1985 The remainder fraction was further purified and concentrated on IgG-Sepharose which retained the ABP that could be eluted without loss of binding activity. Sepharose 68-77 auxin-binding protein 4 Zea mays 97-100 2991267-3 1985 With the aid of affinity chromatography on auxin-binding protein-Sepharose (ABP-Sepharose) monospecific IgGanti-ABP from rabbit antisera were isolated as judged by immuno-double diffusion test and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 65-74 auxin-binding protein 4 Zea mays 76-79 2991267-3 1985 With the aid of affinity chromatography on auxin-binding protein-Sepharose (ABP-Sepharose) monospecific IgGanti-ABP from rabbit antisera were isolated as judged by immuno-double diffusion test and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 65-74 auxin-binding protein 4 Zea mays 112-115 4049326-1 1985 DesAA-fibrin Sepharose was produced by treating fibrinogen-Sepharose with batroxobin. Sepharose 13-22 fibrinogen beta chain Homo sapiens 48-58 4049326-1 1985 DesAA-fibrin Sepharose was produced by treating fibrinogen-Sepharose with batroxobin. Sepharose 59-68 fibrinogen beta chain Homo sapiens 48-58 3861610-12 1985 The carboxylic ionophore, monensin, caused the accumulation of incompletely O-glycosylated glycophorin A molecules, which bound to H. pomatia lectin-Sepharose. Sepharose 149-158 glycophorin A (MNS blood group) Homo sapiens 91-104 3929855-1 1985 Interaction of human low-density lipoproteins (LDL) with homologous fibronectin fixed on collagen-Sepharose was studied. Sepharose 98-107 fibronectin 1 Homo sapiens 68-79 3929855-3 1985 Upon passing modified LDL through a fibronectin-collagen-Sepharose column the desorption of fibronectin occurred. Sepharose 57-66 fibronectin 1 Homo sapiens 36-47 2412838-4 1985 Factor H antigen has been isolated from surface radioiodinated and 35S biosynthetically labeled cells using polyclonal anti-factor H-Sepharose columns. Sepharose 133-142 complement factor H Homo sapiens 0-8 4030100-1 1985 Immunochemically identical components were isolated from water-soluble phases of five Staphylococcus aureus strains by affinity chromatography on fibrinogen-linked Sepharose 4B. Sepharose 164-173 fibrinogen beta chain Homo sapiens 146-156 4040531-4 1985 Binding to SHBG and albumin was confirmed by removing SHBG or albumin from the serum with Concanavalin-A Sepharose 4B gel or CM-Affi Gel Blue, respectively. Sepharose 105-117 sex hormone binding globulin Equus caballus 11-15 3936211-0 1985 A standardized method for measuring anti-F VIII: C inhibitors in haemophilia A by coagulation inhibition in agarose gel. Sepharose 108-115 cytochrome c oxidase subunit 8A Homo sapiens 43-47 4018271-1 1985 Human kappa-casein was prepared from whole casein by successive hydroxyapatite and thiol-Sepharose chromatographies. Sepharose 89-98 casein kappa Homo sapiens 6-18 2991267-3 1985 With the aid of affinity chromatography on auxin-binding protein-Sepharose (ABP-Sepharose) monospecific IgGanti-ABP from rabbit antisera were isolated as judged by immuno-double diffusion test and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 80-89 auxin-binding protein 4 Zea mays 76-79 2991267-3 1985 With the aid of affinity chromatography on auxin-binding protein-Sepharose (ABP-Sepharose) monospecific IgGanti-ABP from rabbit antisera were isolated as judged by immuno-double diffusion test and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 80-89 auxin-binding protein 4 Zea mays 112-115 4052045-2 1985 S-Adenosylhomocysteine hydrolase (EC 3.3.1.1) was purified to homogeneity from human placenta by using S-adenosylhomocysteine-agarose affinity chromatography. Sepharose 126-133 adenosylhomocysteinase Homo sapiens 0-32 3927972-0 1985 Agarose gel method: its usefulness in assaying factor VIII inhibitors, evaluating treatment and suggesting a mechanism of action for factor IX concentrates. Sepharose 0-7 cytochrome c oxidase subunit 8A Homo sapiens 54-58 3874722-0 1985 N-[(carbamoylmethyl)amino]ethanesulfonic acid improves phenotyping of alpha 1-antitrypsin by isoelectric focusing on agarose gel. Sepharose 117-124 serpin family A member 1 Homo sapiens 70-89 3876685-2 1985 The method was based on Ca2+ dependent fixation of C1qrs to agarose, followed by immune precipitation of dissociated C1s in the presence of EDTA. Sepharose 60-67 heterogeneous nuclear ribonucleoprotein C Homo sapiens 51-53 3874722-3 1985 I describe a method in which the ultrathin agarose gel contains N-[(carbamoylmethyl)amino]ethanesulfonic acid as a "separator," to flatten the pH gradient and improve separation of the alpha 1-antitrypsin isoforms. Sepharose 43-50 serpin family A member 1 Homo sapiens 185-204 4018098-3 1985 The two beta-glucocerebrosidases can be distinguished on the basis of their ability to react with anti-(placental beta-glucocerebrosidase) antibodies bound to protein-A--Sepharose 4B beads. Sepharose 170-179 glucosylceramidase beta Homo sapiens 8-31 2417829-7 1985 The affinity with Ricin-Sepharose indicated that most of the FS-alpha and some of the TM-alpha may contain terminal sialic acid and the penultimate structure, Gal beta 1----4G1cNAc; the affinity with PNA-Sepharose indicated that both may also contain terminal sialic acid and the penultimate structure, Gal beta 1----3GalNAc. Sepharose 24-33 bridge-like lipid transfer protein family member 1 Homo sapiens 61-69 4031059-10 1985 Sepharose 2B chromatography revealed that 2ME released a high molecular weight mucin-bilirubin complex as well as unbound pigment from the insoluble matrix. Sepharose 0-9 LOC100508689 Homo sapiens 79-84 4065099-1 1985 Acidic fibroblast growth factor (FGF) from bovine brain has been isolated by a combination of salt precipitation, ion-exchange chromatography, heparin-Sepharose affinity chromatography and reverse phase h.p.l.c. Sepharose 151-160 fibroblast growth factor 1 Bos taurus 0-31 3897285-2 1985 In previous studies, we purified a 55,000-mol wt organic anion-binding protein (OABP) by affinity chromatography on sulfobromophthalein (BSP)-Sepharose of deoxycholate solubilized liver cell plasma membrane preparations. Sepharose 142-151 ATP binding cassette subfamily E member 1 Rattus norvegicus 80-84 3897285-5 1985 Although OABP has not as yet been isolated from each of these tissues and characterized, OABP in heart retained the ability to bind organic anions, and was purified by affinity chromatography on BSP-sepharose. Sepharose 199-208 ATP binding cassette subfamily E member 1 Rattus norvegicus 89-93 3862732-9 1985 Changes in the distribution of apoE were confirmed using agarose gel column chromatography followed by electroimmunoassay. Sepharose 57-64 apolipoprotein E Rattus norvegicus 31-35 3927048-7 1985 By chromatography in concanavalin A-Sepharose 4B, the enzyme can be resolved into two components (F-1 and F-2). Sepharose 36-45 coagulation factor II, thrombin Homo sapiens 98-109 3930644-3 1985 Chromatography of sera on 6% agarose columns showed that apoA-IV is present on HDL and as so-called "free" apoA-IV. Sepharose 29-36 apolipoprotein A4 Rattus norvegicus 57-64 3930645-3 1985 Among normotriglyceridemic subjects, the B protein values in whole plasma obtained by RID using 1.5 to 2.5% agarose were only slightly higher than the values in the d greater than 1.019 g/ml fraction obtained by RID and closely approximated the values obtained in the d greater than 1.019 g/ml fraction by radioimmunoassay. Sepharose 108-115 tyrosinase related protein 1 Homo sapiens 41-50 3930645-4 1985 However, among the hypertriglyceridemic subjects, the RID measurement of B protein in plasma using 1.0 to 2.5% agarose overestimated the LDL B protein levels in plasma. Sepharose 111-118 tyrosinase related protein 1 Homo sapiens 73-82 3930645-5 1985 The RID procedure at agarose concentrations of 1.5% to 2.5% can be used to estimate plasma LDL B protein levels in normotriglyceridemic subjects. Sepharose 21-28 component of oligomeric golgi complex 1 Homo sapiens 91-96 3894594-4 1985 It binds to gelatin- and heparin-coupled Sepharose and it is recognized by specific anti-fibronectin sera. Sepharose 41-50 fibronectin 1 Homo sapiens 89-100 4047041-1 1985 The two common genetic variants of human C3, C3 S and C3 F, were purified and characterized by SDS-PAGE, agarose gel electrophoresis, isoelectric focusing and amino acid analysis. Sepharose 105-112 complement C3 Homo sapiens 41-58 2410911-4 1985 Therefore, in a number of cases, substantially higher amounts of IFN were found after purification by affinity chromatography using concanavalin A, Cibacron blue, or antiserum to IFN-alpha, each coupled to Sepharose. Sepharose 206-215 interferon alpha 1 Homo sapiens 65-68 2861857-3 1985 Together with these heparin fractions, the following three series of heparin samples were examined to compare the affinity for fibronectin-Sepharose: four fractions separated on Sephadex G-100; five fractions separated on antithrombin III-Sepharose, and six partially and completely N-desulfated heparins. Sepharose 139-148 fibronectin 1 Homo sapiens 127-138 3902077-1 1985 A serine protease from sea urchin eggs has been isolated by affinity chromatography on soybean trypsin inhibitor-agarose. Sepharose 113-120 kunitz trypsin protease inhibitor Glycine max 95-112 4040396-1 1985 Tissue plasminogen activator produced by a human melanoma cell line (Bowes), was purified from large volumes of supernatant fluid using immunosorbent chromatography on monoclonal antibodies, followed by chromatography on lysine-Sepharose 4B and gel filtration on Sephadex G-150. Sepharose 228-240 chromosome 20 open reading frame 181 Homo sapiens 0-28 3875078-2 1985 Micro-injection of specific polyA+ RNA fractions in Xenopus laevis oocytes provoked the synthesis of a vWF-like product which could be detected with an immunoradiometric assay relying on Sepharose-linked monoclonal anti-vWF IgG and different radiolabeled monoclonal anti-vWF IgGs. Sepharose 187-196 von Willebrand factor Homo sapiens 103-106 2990610-2 1985 The human active granulocyte-macrophage colony-stimulating factor (GM-CSF) for day 7 CFU-GM and the GM-DF for WEHI-3B(D+) and for HL-60 are not separable by acrylamide agarose column chromatography, eluting at an apparent molecular weight between 20,000 and 35,000 daltons, or by isoelectric focusing (isoelectric point, pH 5.4). Sepharose 168-175 colony stimulating factor 2 Homo sapiens 67-73 2996058-1 1985 Two isoenzymes of oxytocinase (EC 3.4.11.3) activity were fractionated from human amniotic fluid samples between the 14th and 22nd weeks of gestation by Ultrogel acrylamide-agarose gel filtration and partially characterized. Sepharose 173-180 leucyl and cystinyl aminopeptidase Homo sapiens 18-29 3840034-7 1985 The apoE mRNAs appear to be intact and migrate on an agarose gel under denaturing conditions at approximately 18 S. To assay for the biological activity of the apoE mRNAs in these tissues, they were translated in a reticulocyte lysate system in vitro. Sepharose 53-60 apolipoprotein E Homo sapiens 4-8 4041438-1 1985 A calmodulin-sensitive adenylate cyclase was purified 3000-fold from bovine cerebral cortex using DEAE-Sephacel, calmodulin-Sepharose, and two heptanediamine-Sepharose column steps. Sepharose 124-133 calmodulin Bos taurus 2-12 4041438-1 1985 A calmodulin-sensitive adenylate cyclase was purified 3000-fold from bovine cerebral cortex using DEAE-Sephacel, calmodulin-Sepharose, and two heptanediamine-Sepharose column steps. Sepharose 158-167 calmodulin Bos taurus 2-12 3160727-4 1985 In the present study, the digestion of vWF multimers by plasmin was analyzed by sodium dodecyl sulfate-agarose gel electrophoresis and radioimmunoblotting. Sepharose 103-110 von Willebrand factor Homo sapiens 39-42 4022778-1 1985 Mouse fibroblasts labeled 1-9 h with 3H-uridine contained radioactive 45S rRNA subspecies of 13.9, 13.3, and 12.8 kb, as determined by hybrid-selection with rDNA plasmids and by electrophoresis in agarose-formaldehyde. Sepharose 197-204 DNA segment, 45S Mus musculus 70-73 3873347-5 1985 Evidence for direct binding of CSF to gangliosides was obtained by affinity chromatography of CSF on gangliosides-sepharose beads. Sepharose 114-123 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 31-34 3873347-5 1985 Evidence for direct binding of CSF to gangliosides was obtained by affinity chromatography of CSF on gangliosides-sepharose beads. Sepharose 114-123 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 94-97 3160727-4 1985 In the present study, the digestion of vWF multimers by plasmin was analyzed by sodium dodecyl sulfate-agarose gel electrophoresis and radioimmunoblotting. Sepharose 103-110 plasminogen Homo sapiens 56-63 3900092-2 1985 An antibody to the 26,000 dalton light chain of myosin (MLC1), has been produced and purified on a Sepharose 4B affinity column prepared with rat heart myosin. Sepharose 99-108 modulator of VRAC current 1 Rattus norvegicus 56-60 2413010-2 1985 The antibody, named 38 (mAb38, IgG1), was purified from mouse ascites fluid by chromatography on a protein A-Sepharose column. Sepharose 109-118 LOC105243590 Mus musculus 31-35 3839944-4 1985 PF1 eluted from a Sepharose CL-4B column in the same void volume fractions as PF3, phospholipid, and vesicular particles. Sepharose 18-27 PHD finger protein 12 Homo sapiens 0-3 4035648-1 1985 Thrombospondin released from human blood platelets by thrombin activation formed high molecular weight aggregates which co-eluted with haemagglutinin activity on Sepharose 4B gel filtration. Sepharose 162-171 coagulation factor II, thrombin Homo sapiens 54-62 4035652-6 1985 Fibrinogen subjected to gelatin-Sepharose chromatography or dialysis against 3.3M urea reacted equivalently with H3. Sepharose 32-41 fibrinogen beta chain Homo sapiens 0-10 3997878-2 1985 MHC was alkylated with N-ethylmaleimide, purified by Sepharose-4B chromatography, and cleaved with cyanogen bromide. Sepharose 53-65 major histocompatibility complex, class I, C Homo sapiens 0-3 3161540-0 1985 Quantitative characterization of the binding of plasminogen to intact fibrin clots, lysine-sepharose, and fibrin cleaved by plasmin. Sepharose 91-100 plasminogen Homo sapiens 48-55 3161540-5 1985 Limited digestion of fibrin by plasmin created additional binding sites for plasminogen with Kd values similar to the binding of plasminogen to lysine-Sepharose. Sepharose 151-160 plasminogen Homo sapiens 31-38 3928664-2 1985 Human placental NADPH-cytochrome P-450 reductase, obtained by 2",5"-ADP-Sepharose affinity chromatography, was separated into two reductase-active peaks on a Pharmacia Mono-Q column. Sepharose 72-81 cytochrome p450 oxidoreductase Homo sapiens 16-48 2987237-0 1985 Selective affinity chromatography with calmodulin fragments coupled to sepharose. Sepharose 71-80 calmodulin 1 Homo sapiens 39-49 2987237-1 1985 Calmodulin tryptic fragments 78-148, 107-148, and 1-77 coupled to Sepharose 4B were used to test the ability of different calmodulin-regulated enzymes to recognize different domains of calmodulin. Sepharose 66-75 calmodulin 1 Homo sapiens 0-10 2987237-9 1985 These results confirm the multiple modes of interaction of calmodulin with its target proteins and provide the basis for a selective purification of calmodulin-regulated enzymes by affinity chromatography on specific calmodulin fragments coupled to Sepharose. Sepharose 249-258 calmodulin 1 Homo sapiens 59-69 2987237-9 1985 These results confirm the multiple modes of interaction of calmodulin with its target proteins and provide the basis for a selective purification of calmodulin-regulated enzymes by affinity chromatography on specific calmodulin fragments coupled to Sepharose. Sepharose 249-258 calmodulin 1 Homo sapiens 149-159 2987237-9 1985 These results confirm the multiple modes of interaction of calmodulin with its target proteins and provide the basis for a selective purification of calmodulin-regulated enzymes by affinity chromatography on specific calmodulin fragments coupled to Sepharose. Sepharose 249-258 calmodulin 1 Homo sapiens 149-159 4008652-7 1985 Rocket immunoelectrophoresis of mixtures of the purified radiolabeled proteins into anti-Plg containing agarose also confirmed trimolecular complex formation. Sepharose 104-111 plasminogen Homo sapiens 89-92 2409202-7 1985 Expansion of reactive normal cells by S511-Sepharose permitted the development of IL-2-dependent T cell lines enriched for S511-bearing cells. Sepharose 43-52 interleukin 2 Homo sapiens 82-86 2580903-6 1985 Adsorption of detergent lysates of L929 cells with C3bgp-Sepharose depleted by 44% the antigen recognized by anti-human CR1; the C3bgp-binding protein and the cross-reactive antigen exhibited similar patterns on two-dimensional gel electrophoresis; and the isolated C3b-binding protein could be immunoprecipitated with anti-human CR1. Sepharose 57-66 complement receptor 2 Mus musculus 120-123 2580903-6 1985 Adsorption of detergent lysates of L929 cells with C3bgp-Sepharose depleted by 44% the antigen recognized by anti-human CR1; the C3bgp-binding protein and the cross-reactive antigen exhibited similar patterns on two-dimensional gel electrophoresis; and the isolated C3b-binding protein could be immunoprecipitated with anti-human CR1. Sepharose 57-66 endogenous retrovirus group K member 3 Homo sapiens 51-54 2580903-6 1985 Adsorption of detergent lysates of L929 cells with C3bgp-Sepharose depleted by 44% the antigen recognized by anti-human CR1; the C3bgp-binding protein and the cross-reactive antigen exhibited similar patterns on two-dimensional gel electrophoresis; and the isolated C3b-binding protein could be immunoprecipitated with anti-human CR1. Sepharose 57-66 complement receptor 2 Mus musculus 330-333 2859355-9 1985 The human BBB plasma membranes were solubilized in Triton X-100 and were adsorbed to a wheat germ agglutinin Sepharose affinity column, indicating the BBB insulin receptor is a glycoprotein. Sepharose 109-118 insulin Homo sapiens 155-162 4021478-4 1985 The procedure allowed significant purification of progesterone receptor: SDS-polyacrylamide gel electrophoresis of the purified preparation revealed elimination of many peptide bands present in the cytosol prior to ATA-Sepharose chromatography. Sepharose 219-228 progesterone receptor Gallus gallus 50-71 2581953-2 1985 Lipoprotein lipase was purified from bovine skim milk by chromatography on heparin-Sepharose. Sepharose 83-92 lipoprotein lipase Bos taurus 0-18 2581953-6 1985 Incubation of partially purified lipoprotein lipase for 24 h at 37 degrees C results in breakdown of the 55,000-dalton protein with concomitant enrichment in lower Mr components; the proteolytic activity is prevented by incubating the milk with phenylmethane, sulfonyl fluoride prior to chromatography on heparin-Sepharose. Sepharose 313-322 lipoprotein lipase Bos taurus 33-51 2987207-4 1985 Myocardial lipoprotein lipase in the 40,000 X g supernatant fraction was then removed by heparin-Sepharose affinity chromatography. Sepharose 97-106 lipoprotein lipase Rattus norvegicus 11-29 3873300-0 1985 Phenotyping alpha-1-antitrypsin (alpha 1-AT) variants by isoelectric focusing in agarose and immunoblotting. Sepharose 81-88 serpin family A member 1 Homo sapiens 12-31 3873300-0 1985 Phenotyping alpha-1-antitrypsin (alpha 1-AT) variants by isoelectric focusing in agarose and immunoblotting. Sepharose 81-88 serpin family A member 1 Homo sapiens 33-43 3891747-1 1985 The 24-kilodalton messenger RNA cap-binding protein (CBP) was purified from the rabbit reticulocyte postribosomal supernatant fraction using an affinity resin consisting of the p-aminophenyl gamma-ester of m7GTP coupled to Sepharose. Sepharose 223-232 eukaryotic translation initiation factor 4E Homo sapiens 32-51 3891747-1 1985 The 24-kilodalton messenger RNA cap-binding protein (CBP) was purified from the rabbit reticulocyte postribosomal supernatant fraction using an affinity resin consisting of the p-aminophenyl gamma-ester of m7GTP coupled to Sepharose. Sepharose 223-232 eukaryotic translation initiation factor 4E Homo sapiens 53-56 3891747-7 1985 The CBP to ribosome ratio was found to be approximately 0.02, assuming that the m7GTP-Sepharose retains all of the protein. Sepharose 86-95 eukaryotic translation initiation factor 4E Homo sapiens 4-7 4068572-1 1985 Fibronectin was isolated from a patient"s plasma by affinity chromatography using gelatin-agarose. Sepharose 90-97 fibronectin 1 Homo sapiens 0-11 2931900-1 1985 Plasmin, free of an activator, was obtained after activation of the highly purified human plasminogen by means of trypsin immobilized on Sepharose 4B and after removal of the enzyme from the system. Sepharose 137-149 plasminogen Homo sapiens 0-7 3925020-2 1985 Anti-human ADA antibody was purified by affinity chromatography on a column of Sepharose 4B to which calf ADA was covalently linked. Sepharose 79-91 adenosine deaminase Bos taurus 11-14 3922430-6 1985 The glycopeptides which bound to Datura-lectin were degraded by endo-beta-galactosidase (or keratanase) to yield Gal----GlcNAc----Gal and glycopeptides, which were resistant to further endo-beta-galactosidase digestion and which no longer bound to Datura lectin-agarose. Sepharose 262-269 galactosidase, beta 1 Mus musculus 69-87 3924105-1 1985 Discrete apolipoprotein E-containing lipoproteins can be identified when EDTA plasma is fractionated on columns of 4% agarose. Sepharose 118-125 apolipoprotein E Homo sapiens 9-25 3890754-4 1985 Chymase was also purified from rat peritoneal cells by employing a one-step method involving hydrophobic chromatography on octyl-Sepharose 4B or arginine-Sepharose 4B. Sepharose 129-138 chymase 1 Rattus norvegicus 0-7 2989336-4 1985 Glycosylation of secreted angiotensinogen was further characterized by using chromatography on concanavalin A-Sepharose, digestion with neuraminidase, and treatment with trifluoromethane sulfonic acid. Sepharose 110-119 angiotensinogen Homo sapiens 26-41 4008652-6 1985 HRGP covalently cross-linked to Sepharose 4B simultaneously bound both 125I-TSP and 131I-Plg, confirming trimolecular complex formation. Sepharose 32-44 histidine rich glycoprotein Homo sapiens 0-4 4008652-6 1985 HRGP covalently cross-linked to Sepharose 4B simultaneously bound both 125I-TSP and 131I-Plg, confirming trimolecular complex formation. Sepharose 32-44 thrombospondin 1 Homo sapiens 76-79 4008652-6 1985 HRGP covalently cross-linked to Sepharose 4B simultaneously bound both 125I-TSP and 131I-Plg, confirming trimolecular complex formation. Sepharose 32-44 plasminogen Homo sapiens 89-92 2993464-6 1985 On agarose gel electrophoresis, pH 8.6, the nitrated HDL3 moved ahead of the control HDL3, indicating an increase in negative charges in the molecule. Sepharose 3-10 HDL3 Homo sapiens 53-57 3921614-3 1985 They are interleukin 2-conditioned medium (CM-IL 2), which was produced by W/Fu rat spleen cells cultured with concanavalin A-conjugated Sepharose beads, and cytotoxic cell differentiation factor-conditioned medium (CM-CCDF), which was produced primarily by the unstimulated mouse peritoneal macrophages. Sepharose 137-146 interleukin 2 Rattus norvegicus 46-50 4023466-5 1985 This macromolecular PRL was also bound specifically to Sepharose coupled with anti-PRL, indicating that this macromolecule contained a sequence of PRL in its structure. Sepharose 55-64 prolactin Homo sapiens 20-23 4023466-5 1985 This macromolecular PRL was also bound specifically to Sepharose coupled with anti-PRL, indicating that this macromolecule contained a sequence of PRL in its structure. Sepharose 55-64 prolactin Homo sapiens 83-86 4023466-5 1985 This macromolecular PRL was also bound specifically to Sepharose coupled with anti-PRL, indicating that this macromolecule contained a sequence of PRL in its structure. Sepharose 55-64 prolactin Homo sapiens 83-86 4022016-10 1985 Absorption of thyroiditis sera on thyroglobulin-Sepharose followed by immunoprecipitation abolished the anti-thyroglobulin components without affecting the binding of the 105,000-dalton polypeptide, if the sera contained antimicrosomal antibody. Sepharose 48-57 thyroglobulin Homo sapiens 34-47 3927518-2 1985 The activities of beta-N-acetylglucosaminidase, beta-galactosidase and beta-glucosidase in the former were distinguished from those in the latter on the basis of solubilization pattern, pH optimum, thermal stability and elution pattern of concanavalin A-Sepharose chromatography. Sepharose 254-263 galactosidase, beta 1 Rattus norvegicus 48-66 3894064-7 1985 Affinity chromatography of the pituitary renin on a Concanavalin-Sepharose column showed that most (87.4%) of the pituitary renin did not contain glycoprotein residues. Sepharose 65-74 renin Homo sapiens 41-46 3886660-3 1985 The hABP in extracts of human testes was composed of two molecular species based on concanavalin A (ConA)-Sepharose chromatography. Sepharose 106-115 sex hormone binding globulin Homo sapiens 4-8 2986784-6 1985 Affinity chromatography of an organelle-enriched brain fraction was carried out using a caseinyl-sepharose column and resulted in the separation of renin from cathepsin D activity; the renin peak was inhibited by antibodies raised against rat kidney renin. Sepharose 97-106 renin Rattus norvegicus 148-153 2986784-6 1985 Affinity chromatography of an organelle-enriched brain fraction was carried out using a caseinyl-sepharose column and resulted in the separation of renin from cathepsin D activity; the renin peak was inhibited by antibodies raised against rat kidney renin. Sepharose 97-106 renin Rattus norvegicus 185-190 4005324-5 1985 In a model system involving mitochondrial enzyme preparations (cytochrome c, glutamate dehydrogenase, isocitrate dehydrogenase, catalase), the possibility of their adsorption of F-actin-sepharose was investigated. Sepharose 186-195 catalase Rattus norvegicus 128-136 2986784-6 1985 Affinity chromatography of an organelle-enriched brain fraction was carried out using a caseinyl-sepharose column and resulted in the separation of renin from cathepsin D activity; the renin peak was inhibited by antibodies raised against rat kidney renin. Sepharose 97-106 renin Rattus norvegicus 185-190 3922451-7 1985 P 5-2-1 immobilized on Sepharose can be used to deplete HF from normal human plasma. Sepharose 23-32 coagulation factor XII Homo sapiens 56-58 4005324-5 1985 In a model system involving mitochondrial enzyme preparations (cytochrome c, glutamate dehydrogenase, isocitrate dehydrogenase, catalase), the possibility of their adsorption of F-actin-sepharose was investigated. Sepharose 186-195 actin Oryctolagus cuniculus 180-185 3006746-6 1985 Chlorpromazine-Sepharose affinity chromatography of peptide/calmodulin adducts showed that a significant portion of the cross-linked beta-endorphin 14-31/calmodulin complex (stoichiometry of 1 mol/mol) retained the ability to interact with the immobilized phenothiazine in a Ca2+-dependent and calmodulin-displaceable manner. Sepharose 15-24 proopiomelanocortin Bos taurus 133-147 3872340-2 1985 Like the pI 6.8 species of IL-1 from human peripheral blood monocytes (PBM), the cell line IL-1 has a molecular weight (MW) of 14,000, a pI of 6.8, is heat labile, and does not bind to concanavalin A-Sepharose. Sepharose 200-209 interleukin 1 alpha Homo sapiens 91-95 3894653-1 1985 Boar sperm acrosin isolated by affinity chromatography on p-(p"-aminophenoxypropoxy)benzamidine linked to Sepharose was tested for its proteolytic effect on the zona pellucida of freshly ovulated pig eggs. Sepharose 106-115 acrosin Sus scrofa 11-18 2582237-15 1985 The focus-selected c-src overexpressed cells had altered morphology and limited growth in soft agarose but were not tumorigenic in vivo. Sepharose 95-102 SRC proto-oncogene, non-receptor tyrosine kinase Gallus gallus 19-24 3858814-10 1985 Calmodulin-Sepharose affinity chromatography in the presence of 2 M urea indicates that the interaction of peptide and calmodulin is Ca2+-dependent. Sepharose 11-20 calmodulin Oryctolagus cuniculus 0-10 3858814-10 1985 Calmodulin-Sepharose affinity chromatography in the presence of 2 M urea indicates that the interaction of peptide and calmodulin is Ca2+-dependent. Sepharose 11-20 calmodulin Oryctolagus cuniculus 119-129 3922419-1 1985 Apolipoprotein A-IV was isolated from the d less than 1.21 g/ml fraction of rat serum by gel filtration followed by heparin-Sepharose affinity chromatography; this method also facilitated the preparation of apolipoprotein A-I and apolipoprotein E. Sepharose 124-133 apolipoprotein A4 Rattus norvegicus 0-19 4040658-7 1985 t-PA was desorbed from antibody HI72A1-Sepharose columns with 0.5 M NaCl, consistent with its relatively low association constant (Ka = 9.37 X 10(7) M-1). Sepharose 39-48 plasminogen activator, tissue type Homo sapiens 0-4 2579948-5 1985 In this assay these complexes are removed from plasma by immunoabsorption with the IgG fraction of rabbit anti-alpha 2-macroglobulin antiserum coupled to an agarose gel. Sepharose 157-164 alpha-2-macroglobulin Homo sapiens 111-132 4039542-1 1985 Rapid and selective removal of plasma vitamin D-binding protein was effected by the serial passage of plasma over four columns of agarose containing covalently linked skeletal muscle G-actin. Sepharose 130-137 GC vitamin D binding protein Homo sapiens 38-63 2985302-5 1985 Pro-opiomelanocortin (POMC) related peptides in the plasma and tumour tissue extract of this patient have been characterized by gel-filtration and Concanavalin-A Sepharose affinity chromatography, indicating processing of POMC in a manner more usually associated with ectopic tumours. Sepharose 162-171 proopiomelanocortin Homo sapiens 0-20 2985302-5 1985 Pro-opiomelanocortin (POMC) related peptides in the plasma and tumour tissue extract of this patient have been characterized by gel-filtration and Concanavalin-A Sepharose affinity chromatography, indicating processing of POMC in a manner more usually associated with ectopic tumours. Sepharose 162-171 proopiomelanocortin Homo sapiens 22-26 3872166-0 1985 Cell-mediated inhibition of tumor colony formation in agarose by resting and interleukin 2-stimulated human lymphocytes. Sepharose 54-61 interleukin 2 Homo sapiens 77-90 2412807-4 1985 Furthermore, when the translation mixture was applied to a cortisol-Sepharose column, the translated transcortin was bound to the matrix in a specific manner, indicating that this product binds to cortisol. Sepharose 68-77 serpin family A member 6 Homo sapiens 101-112 3999012-3 1985 Extensive in vitro enzymatic desialylation of isolated human AAG was achieved utilizing neuraminidase bonded to agarose beads. Sepharose 112-119 neuraminidase 1 Homo sapiens 88-101 4046212-2 1985 Therefore, PNA-Sepharose affinity chromatography is an effective tool for the detection of desialylated hCG and may be useful also for the diagnosis of choriocarcinoma. Sepharose 15-24 chorionic gonadotropin subunit beta 5 Homo sapiens 104-107 3882608-4 1985 The monospecific immunoglobulins to HIOMT were isolated by positive-negative selection using pineal extract-sepharose and brain extract-sepharose affinity chromatography. Sepharose 108-117 acetylserotonin O-methyltransferase Homo sapiens 36-41 3882608-4 1985 The monospecific immunoglobulins to HIOMT were isolated by positive-negative selection using pineal extract-sepharose and brain extract-sepharose affinity chromatography. Sepharose 136-145 acetylserotonin O-methyltransferase Homo sapiens 36-41 3926759-2 1985 They were named RNase K1 and RNase K2 in order of elution from the heparin-Sepharose column. Sepharose 75-84 ribonuclease A family member k6 Bos taurus 29-37 2578523-2 1985 The glycosylation-enhancing factor (GEF) is a kallikrein-like enzyme and is purified by absorption to p-aminobenzamidine-Agarose followed by elution with benzamidine. Sepharose 121-128 rho/rac guanine nucleotide exchange factor (GEF) 2 Mus musculus 36-39 2578523-2 1985 The glycosylation-enhancing factor (GEF) is a kallikrein-like enzyme and is purified by absorption to p-aminobenzamidine-Agarose followed by elution with benzamidine. Sepharose 121-128 kallikrein 1-related peptidase b9 Mus musculus 46-56 3856846-1 1985 Epoxide hydrolase from liver cytosol (cEH) of both normal and clofibrate-treated mice can be bioselectively adsorbed onto an affinity column prepared from epoxy-activated Sepharose and 7-methoxycitronellyl thiol. Sepharose 171-180 epoxide hydrolase 2, cytoplasmic Mus musculus 38-41 2982422-7 1985 A virtually identical calmodulin-dependent protein kinase activity was purified from rat liver by affinity chromatography on calmodulin-Sepharose. Sepharose 136-145 calmodulin 1 Rattus norvegicus 22-32 2982422-7 1985 A virtually identical calmodulin-dependent protein kinase activity was purified from rat liver by affinity chromatography on calmodulin-Sepharose. Sepharose 136-145 calmodulin 1 Rattus norvegicus 125-135 2985615-1 1985 When a partially purified insulin receptor preparation immobilized on insulin-agarose is incubated with [gamma-32P]ATP, Mn2+, and Mg2+ ions, the receptor beta subunit becomes 32P-labeled. Sepharose 78-85 insulin receptor Homo sapiens 26-42 2985615-8 1985 Subsequent to src kinase-mediated tyrosine phosphorylation, the insulin receptor, either immobilized on insulin-agarose or in detergent extracts, exhibits a 2-fold increase in associated kinase activity using histone as substrate. Sepharose 112-119 insulin receptor Homo sapiens 64-80 3859706-1 1985 Human placental estrogen sulfotransferase (ESFT) was partially purified from the term placental cytosol by (NH4)2SO4 precipitation and agarose gel chromatography. Sepharose 135-142 sulfotransferase family 1A member 3 Homo sapiens 6-41 16664214-5 1985 Nitrate-grown nr(1) mutant soybean plants yielded a NADH:nitrate reductase (designated iNR) when Blue Sepharose columns were eluted with NADH; NADPH failed to elute any NR form from Blue Sepharose loaded with this extract. Sepharose 102-111 chalcone reductase CHR1 Glycine max 65-74 3988735-1 1985 Bovine milk lipase was noncovalently bound to a heparin-Sepharose support and a [3H]glycerol/[14C]triolein emulsion was circulated through it. Sepharose 56-65 carboxyl ester lipase Homo sapiens 7-18 3988735-3 1985 Both apo-C-II and apo-E produced enhanced lipolysis in comparison to unsupplemented emulsions, at appropriate enzyme densities on the heparin-Sepharose. Sepharose 142-151 apolipoprotein C2 Homo sapiens 5-13 3988735-3 1985 Both apo-C-II and apo-E produced enhanced lipolysis in comparison to unsupplemented emulsions, at appropriate enzyme densities on the heparin-Sepharose. Sepharose 142-151 apolipoprotein E Homo sapiens 18-23 3988735-6 1985 The enhancement of lipolysis produced by apo-E was correlated with the increased binding of triglyceride to the heparin-Sepharose enzyme complex. Sepharose 120-129 apolipoprotein E Homo sapiens 41-46 3872795-2 1985 By attaching native collagen and C1q to Sepharose, it was possible to test the binding of fibronectin (Fn) to the native and heat-denatured forms of these proteins without complications due to aggregation, precipitation, or fibril formation. Sepharose 40-49 fibronectin 1 Homo sapiens 90-101 3872795-6 1985 C1q bound readily to native IgG-Sepharose but did not mediate the binding of Fn. Sepharose 32-41 complement C1q A chain Homo sapiens 0-3 3920213-7 1985 1) Upon urea gradient elution of affinity-bound fibronectin fragments from gelatin-Sepharose chromatography, the apex of the elution peak for polylactosamine-containing fragments occurs at 2.0 M urea while the peak for complex N-linked carbohydrate-containing fragments maximized at 2.5 M urea indicating a tighter binding. Sepharose 83-92 fibronectin 1 Homo sapiens 48-59 3993931-2 1985 A galactosyl-Sepharose affinity matrix was used to bind ricin toxin and its associated agglutinin, which both bind specifically to galactose, from a crude extract. Sepharose 13-22 ricin Ricinus communis 56-61 3919966-0 1985 Immunoenzymometric assay for insulin involving column chromatography and insulin immobilized on Sepharose. Sepharose 96-105 insulin Homo sapiens 29-36 3919966-0 1985 Immunoenzymometric assay for insulin involving column chromatography and insulin immobilized on Sepharose. Sepharose 96-105 insulin Homo sapiens 73-80 2985718-3 1985 Both standard hCG and hCG-beta adsorbed to the PNA-Sepharose only when they were treated with neuraminidase, whereas hCG-alpha was still not adsorbed even after treatment with neuraminidase. Sepharose 51-60 chorionic gonadotropin subunit beta 5 Homo sapiens 14-17 2985718-3 1985 Both standard hCG and hCG-beta adsorbed to the PNA-Sepharose only when they were treated with neuraminidase, whereas hCG-alpha was still not adsorbed even after treatment with neuraminidase. Sepharose 51-60 chorionic gonadotropin subunit beta 3 Homo sapiens 22-30 2985718-3 1985 Both standard hCG and hCG-beta adsorbed to the PNA-Sepharose only when they were treated with neuraminidase, whereas hCG-alpha was still not adsorbed even after treatment with neuraminidase. Sepharose 51-60 neuraminidase 1 Homo sapiens 94-107 2985718-8 1985 These results suggest that PNA-Sepharose affinity chromatography is an effective tool to use in detecting incompletely sialylated hCG and hCG-beta and may be a useful index for the clinical diagnosis of choriocarcinoma. Sepharose 31-40 chorionic gonadotropin subunit beta 5 Homo sapiens 130-133 2985718-8 1985 These results suggest that PNA-Sepharose affinity chromatography is an effective tool to use in detecting incompletely sialylated hCG and hCG-beta and may be a useful index for the clinical diagnosis of choriocarcinoma. Sepharose 31-40 chorionic gonadotropin subunit beta 3 Homo sapiens 138-146 3856880-4 1985 Antibody purified with the Sepharose 4B conjugate from mixed antiserum to P-450(16) alpha and P-450(15) alpha specifically inhibited testosterone 16 alpha-hydroxylase activity in microsomes. Sepharose 27-36 cytochrome P450, family 2, subfamily b, polypeptide 10 Mus musculus 133-166 3919027-7 1985 The 67-kDa protein kinase, uninducible by treatment with alpha, beta IFN (up to 13,000 units/ml), is instead induced upon treatment with gamma IFN at a similar rate of activity as in wild-type Friend leukemia cells, both when assayed in solution and after immobilization on poly(rI) X poly(rC)-agarose. Sepharose 294-301 interferon gamma Mus musculus 143-146 3919773-2 1985 The first stage of the purification of the lipoprotein lipase was carried out with heparin-Sepharose affinity chromatography. Sepharose 91-100 lipoprotein lipase Rattus norvegicus 43-61 2982846-3 1985 The affinity of angiotensin-converting enzyme for the Sepharose-spacer-lisinopril matrix (Ki matrix = 1 X 10(-5) M) is weak compared to its affinity for free lisinopril (Ki = 1 X 10(-10) M). Sepharose 54-63 angiotensin-converting enzyme Oryctolagus cuniculus 16-45 3994667-7 1985 Binding of active lipoprotein lipase to heparin-Sepharose could be demonstrated at pH down to 6.5. Sepharose 48-57 lipoprotein lipase Homo sapiens 18-36 3994667-11 1985 Catalytically inactive lipoprotein lipase retained the ability to bind to heparin-Sepharose. Sepharose 82-91 lipoprotein lipase Homo sapiens 23-41 4065238-0 1985 Simplified concanavalin-A sepharose adsorption method for separation of cone visual pigments from rhodopsin. Sepharose 26-35 rhodopsin Gallus gallus 98-107 4038696-5 1985 IRBP can be isolated rapidly from IPM by affinity adsorption onto con A-Sepharose; this preparation is 94% pure and yields 0.33 mg or 2.4 nmol of IRBP per bovine eye. Sepharose 72-81 retinol binding protein 3 Bos taurus 0-4 3156882-6 1985 When solubilized radioiodinated cells were chromatographed on a column of agarose-bound B2.12, a single radiolabeled protein was obtained whose apparent molecular weight is slightly larger than that of platelet GP IIIa. Sepharose 74-81 integrin subunit beta 3 Homo sapiens 211-218 3006746-6 1985 Chlorpromazine-Sepharose affinity chromatography of peptide/calmodulin adducts showed that a significant portion of the cross-linked beta-endorphin 14-31/calmodulin complex (stoichiometry of 1 mol/mol) retained the ability to interact with the immobilized phenothiazine in a Ca2+-dependent and calmodulin-displaceable manner. Sepharose 15-24 calmodulin Bos taurus 154-164 3006746-6 1985 Chlorpromazine-Sepharose affinity chromatography of peptide/calmodulin adducts showed that a significant portion of the cross-linked beta-endorphin 14-31/calmodulin complex (stoichiometry of 1 mol/mol) retained the ability to interact with the immobilized phenothiazine in a Ca2+-dependent and calmodulin-displaceable manner. Sepharose 15-24 calmodulin Bos taurus 154-164 3882151-3 1985 Lipoprotein lipase in newborns liver was characterized by its inhibition in the presence of 1.0 M NaCl, its specific elution at 1.5 M NaCl on heparin-Sepharose 4B column and its requirement for serum in the assay mixture to manifest its activity. Sepharose 150-159 lipoprotein lipase Rattus norvegicus 0-18 3898040-2 1985 Immunoreactive LRF neurons were precipitated when dispersed cells of adult male rats were incubated successively in media containing free LRF antiserum followed by the exposure of LRF bound to Sepharose-4B. Sepharose 193-202 zinc finger and BTB domain containing 7a Rattus norvegicus 15-18 4039605-2 1985 A very high capacity immunoaffinity matrix for the purification of progesterone receptor was prepared by cross-linking a monoclonal antireceptor antibody to protein A-Sepharose through the Fc fragment. Sepharose 167-176 progesterone receptor Oryctolagus cuniculus 67-88 4038570-6 1985 Anti-hTeBG was titrated using protein A-Sepharose which quantitatively binds IgG and therefore bound [3H]DHT-hTeBG-anti-TeBG complexes. Sepharose 40-49 sex hormone binding globulin Homo sapiens 6-10 2981585-2 1985 By sodium dodecylsulfate-agarose gel electrophoresis, native platelet vWF contained some multimers that were larger than those characteristic of plasma vWF. Sepharose 25-32 von Willebrand factor Homo sapiens 70-73 3994654-0 1985 Mapping of zein polypeptides after isoelectric focusing on agarose gels. Sepharose 59-66 zein Zea mays 11-15 3994654-1 1985 Isoelectric focusing of zein in agarose gels gives sharp separations of at least 25 bands noted among 25 corn-belt inbreds. Sepharose 32-39 zein Zea mays 24-28 3994654-6 1985 Zein isoelectric focusing in agarose should be useful for genotype identification and for determination of varietal purity. Sepharose 29-36 zein Zea mays 0-4 4005311-7 1985 Cibacron Blue purified EDGF was also further fractionated by heparin sepharose. Sepharose 69-78 fibroblast growth factor 1 Bos taurus 23-27 3881194-4 1985 Finally, glycolipids from HL60-TPA cells but not from HL-60 cells were able to reversibly bind MIF when covalently coupled to agarose. Sepharose 126-133 macrophage migration inhibitory factor Homo sapiens 95-98 3882420-6 1985 This protease and the factor altering ER-DNA binding were eluted together from chromatography on DEAE-cellulose, AcA 44, and carboline-agarose and were sensitive to the same inhibitors. Sepharose 135-142 estrogen receptor 1 Bos taurus 38-40 3973671-5 1985 Fibronectin fragments with differing biological activities were produced by proteolytic digestion with trypsin and cathepsin D and sequential affinity chromatography on gelatin-agarose and heparin-Sepharose. Sepharose 177-184 fibronectin 1 Gallus gallus 0-11 3973671-5 1985 Fibronectin fragments with differing biological activities were produced by proteolytic digestion with trypsin and cathepsin D and sequential affinity chromatography on gelatin-agarose and heparin-Sepharose. Sepharose 197-206 fibronectin 1 Gallus gallus 0-11 3982800-2 1985 Fibronectin eyedrops were prepared from the patient"s own blood plasma by an affinity chromatography using gelatin-coupled agarose gel and by gel filtration technique. Sepharose 123-130 fibronectin 1 Homo sapiens 0-11 3919716-1 1985 Slices of human lung cancer tissue were incubated with [32P]-orthophosphoric acid, and the radiolabeled beta-glucuronidase was isolated by a procedure including immunoaffinity chromatography on anti-human liver beta-glucuronidase IgG Sepharose. Sepharose 234-243 glucuronidase beta Homo sapiens 104-122 3967764-2 1985 TNF in 355 ml rabbit serum was precipitated with ammonium sulfate, and purified by repeated DEAE-Sephadex and Sephacryl S-200 chromatographies, and the final fractionation on Blue-Sepharose 6B. Sepharose 180-189 tumor necrosis factor Oryctolagus cuniculus 0-3 3871610-4 1985 At different times after injection alpha 1-proteinase inhibitor was isolated from plasma by affinity chromatography with anti-alpha 1-proteinase inhibitor Sepharose. Sepharose 155-164 serpin family A member 1 Rattus norvegicus 35-63 3871610-4 1985 At different times after injection alpha 1-proteinase inhibitor was isolated from plasma by affinity chromatography with anti-alpha 1-proteinase inhibitor Sepharose. Sepharose 155-164 serpin family A member 1 Rattus norvegicus 126-154 2578390-3 1985 Human actin-depolymerizing factor (ADF) has been purified by ammonium sulfate fractionation, DEAE-cellulose and blue-Sepharose chromatography. Sepharose 117-126 destrin, actin depolymerizing factor Homo sapiens 35-38 2578390-5 1985 ADF is heat and trypsin-sensitive, inactivated by EGTA, not stained by HIO4/Schiff on sodium dodecyl sulfate/polyacrylamide gel electrophoresis (SDS/PAGE), and not retained on a concanavalin-A-Sepharose column. Sepharose 193-202 destrin, actin depolymerizing factor Homo sapiens 0-3 2981214-1 1985 A membrane-associated receptor involved in a specific uptake of formaldehyde-treated serum albumin (f-Alb) was purified from rat livers by Triton X-100 solubilization of a 105,000 X g membrane preparation and affinity chromatography on an f-Alb-Sepharose column. Sepharose 245-254 albumin Rattus norvegicus 102-105 3965464-1 1985 Two distinct forms of antithrombin III were isolated by chromatography of normal human plasma on heparin-Sepharose. Sepharose 105-114 serpin family C member 1 Homo sapiens 22-38 3975871-3 1985 Heparin Sepharose chromatography and heparin cofactor assays suggested that the primary target of lipid peroxides on the At III molecule may be the heparin binding site. Sepharose 8-17 serpin family C member 1 Homo sapiens 121-127 3871334-7 1985 The homogeneity of the purified cytochrome P-450 was examined with the following methods: isoelectric focusing, immunoelectrophoresis and affinity chromatography on cytochrome b5-immobilized Sepharose. Sepharose 191-200 cytochrome b5 Cavia porcellus 165-178 3917935-1 1985 Two-dimensional gel electrophoresis with the 125I-Con A overlay and affinity purification with Con A-agarose revealed the presence of an abundant ubiquitous 100-kDa glycoprotein (GP100) in nucleated mammalin cells. Sepharose 101-108 premelanosome protein Homo sapiens 179-184 3860177-5 1985 Insulin receptor is isolated by formation of a complex between the bifunctional ligand and the receptor, and then adsorption to "monomeric" avidin-Sepharose via the biotin moiety. Sepharose 147-156 insulin receptor Mus musculus 0-16 4096539-2 1985 Pig-skin (epidermal) calmodulin was purified to homogeneity by DEAE/Sepharose- and phenothiazine-affinity-column chromatography. Sepharose 68-77 calmodulin-3 Sus scrofa 21-31 3834881-1 1985 Distribution of PLG types was studied in a sample of the Polish population numbering 230 subjects by the method of high-voltage agarose electrophoresis. Sepharose 128-135 plasminogen Homo sapiens 16-19 4084018-2 1985 CRP was purified using 4-step procedure including absorption on Sepharose 4B, phosphocholine-Sepharose, DE-52 ion exchange chromatography and gel filtration on Sephacryl S-200. Sepharose 64-76 C-reactive protein Homo sapiens 0-3 4084018-2 1985 CRP was purified using 4-step procedure including absorption on Sepharose 4B, phosphocholine-Sepharose, DE-52 ion exchange chromatography and gel filtration on Sephacryl S-200. Sepharose 64-73 C-reactive protein Homo sapiens 0-3 4062572-2 1985 HTGL was separated from the postheparin plasma (PHP) by heparin-Sepharose affinity chromatography. Sepharose 64-73 lipase C, hepatic type Homo sapiens 0-4 2981638-0 1985 Selective inhibitory effect of Hu-IFN-gamma on the agarose clonability of tumor-derived lymphoid cell lines. Sepharose 51-58 interferon gamma Homo sapiens 34-43 2981638-5 1985 In contrast, IFN-gamma had no effect on the growth in suspension but it abolished the clonogenic potential of tumor cell lines in semisolid agarose. Sepharose 140-147 interferon gamma Homo sapiens 13-22 3160465-0 1985 Isolation of beta-hexosaminidase isoenzymes from annelids by phenyl boronate agarose chromatography. Sepharose 77-84 O-GlcNAcase Homo sapiens 13-32 3004808-9 1985 Schiff"s staining on polyacrylamide gel and interaction with Con-A-Sepharose indicate that rat trehalase is a glycoprotein. Sepharose 67-76 trehalase Rattus norvegicus 95-104 3937657-3 1985 When Triton X-100-solubilized microsomes were chromatographed on calmodulin Sepharose, several proteins were found to interact with calmodulin in a Ca2+-dependent manner. Sepharose 76-85 calmodulin 1 Homo sapiens 65-75 3937657-3 1985 When Triton X-100-solubilized microsomes were chromatographed on calmodulin Sepharose, several proteins were found to interact with calmodulin in a Ca2+-dependent manner. Sepharose 76-85 calmodulin 1 Homo sapiens 132-142 4085220-1 1985 Serum depleted of the first component of complement (C1D) was prepared by treating fresh human serum with Sepharose-IgG in the presence of triethylenetetramine-N,N,N",N"",N""",N"""-hexa-acetic acid and di-isopropyl fluorophosphate at acidic pH (5.2). Sepharose 106-115 C1D nuclear receptor corepressor Homo sapiens 53-56 3920008-4 1985 The antibodies could be divided into two groups on the basis of their reactivities towards iodinated alpha-crystallin, their ability to bind to Protein A-Sepharose, their immunoglobulin subclass, their immunoelectrophoretic patterns and their abilities to react with chicken and opossum alpha-crystallin. Sepharose 154-163 G protein-coupled receptor 162 Gallus gallus 144-153 2414096-0 1985 Analysis of guanase by agarose gel electrophoresis and activity staining. Sepharose 23-30 guanine deaminase Homo sapiens 12-19 2414096-1 1985 A method was developed to separate guanase by agarose gel electrophoresis and to detect its activity by staining of the bands with a mixture of the enzymes xanthine oxidase, catalase, and aldehyde dehydrogenase, the coenzyme NADP+, and a substrate of guanine, ethanol, phenazine methosulfate, nitrotetrazolium blue, and KCN in Tris-(hydroxymethyl)methylamine buffer (pH 8.0). Sepharose 46-53 guanine deaminase Homo sapiens 35-42 2581874-2 1985 Fibrinogen was isolated from plasmas by affinity chromatography at fibrin monomer Sepharose and characterized by SDS-PAGE. Sepharose 82-91 fibrinogen beta chain Homo sapiens 0-10 3837725-2 1985 By loading serum on a Protein A-sepharose column, IgG3 are readily recovered as they do not bind to the Protein A. IgG of the other subclasses are eluted, equilibrated at pH 6 and loaded either on a chromatofocusing column, or on an anion exchanger gel. Sepharose 32-41 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 50-54 4085917-5 1985 ABP was further characterized by affinity chromatography on concanavalin A-Sepharose and was found to separate into two parts: one showed no interaction with the resin and was eluted in the void volume (peak I) and the other bound to the resin and was eluted by alpha-methyl-D-glucoside (peak II). Sepharose 75-84 sex hormone binding globulin Homo sapiens 0-3 3157638-0 1985 Phosphoglucomutase (PGM1) subtypes in a Finnish population determined by isoelectric focusing in agarose gel. Sepharose 97-104 phosphoglucomutase 1 Homo sapiens 20-24 3157638-1 1985 The red cell enzyme phosphoglucomutase first locus (PGM1) phenotypes of 639 adult Finns were determined by isoelectric focusing in agarose gel. Sepharose 131-138 phosphoglucomutase 1 Homo sapiens 52-56 3876986-2 1985 Affinity purified C1q was covalently coupled to a newly developed agarose polyacrolein microsphere beads immunoadsorbent. Sepharose 66-73 complement C1q A chain Homo sapiens 18-21 2414230-2 1985 After two successive purifications by preparative isoelectric focusing (IEF), Dg1 was characterized as a single band in analytical agarose IEF with a pI of 5.9 and was found to display 3 bands by sodium dodecyl sulfate polyacrylamide gel with the respective molecular weights: 21,000, 31,000 and 33,000 daltons. Sepharose 131-138 desmoglein 1 Homo sapiens 78-81 2581940-3 1985 Antibody from one clone was coupled to CNBr-activated Sepharose 4B and the monoclonal antibody-Sepharose was shown to be very useful for isolating rat tyrosine 3-monooxygenase from crude preparations. Sepharose 95-104 tyrosine hydroxylase Rattus norvegicus 151-175 3001106-0 1985 Receptors for insulin and epidermal growth factor: interaction with organomercurial agarose. Sepharose 84-91 epidermal growth factor Homo sapiens 14-49 3158644-4 1985 The inhibitory substances from the supernatant of the thrombin-induced aggregates were separated into two major fractions, a low affinity fraction and a high affinity fraction, on a heparin-Sepharose column. Sepharose 190-199 coagulation factor II, thrombin Bos taurus 54-62 4038400-1 1985 The mitochondrial DNA-binding protein P16 was isolated from rat liver mitochondrial lysates by affinity chromatography on single strand DNA agarose and separated from DNA in the preparation by alkaline CsCl isopycnic gradients. Sepharose 140-147 cyclin-dependent kinase inhibitor 2A Rattus norvegicus 38-41 3871109-9 1985 Moreover, it bound to lima bean agglutinin-Sepharose specific for N-acetylgalactosamine residues, indicating that B151-TRF is a glycosylated glycoprotein containing N-acetylgalactosamine residues. Sepharose 43-52 interleukin 5 Mus musculus 119-122 2981098-2 1985 The enzyme was purified 200-250-fold from the homogenate by solubilization with Triton X-100 from microsomal membranes and affinity chromatography on CDP-diacylglycerol-Sepharose. Sepharose 169-178 cut-like homeobox 1 Rattus norvegicus 150-153 3964830-1 1985 Acetylcholinesterase (AChE; EC 3.1.1.7) extracted in 1% Triton X-100 from rabbit brain was purified 2,000-fold by chromatography on agarose conjugated with a monoclonal antibody directed against human red blood cell cholinesterase. Sepharose 132-139 ACE-1 Oryctolagus cuniculus 0-20 3964830-1 1985 Acetylcholinesterase (AChE; EC 3.1.1.7) extracted in 1% Triton X-100 from rabbit brain was purified 2,000-fold by chromatography on agarose conjugated with a monoclonal antibody directed against human red blood cell cholinesterase. Sepharose 132-139 ACE-1 Oryctolagus cuniculus 22-26 6334580-1 1984 IgE was isolated from a patient with the hyper IgE, recurrent infection syndrome by immunoadsorption on sepharose bound goat anti-human IgE. Sepharose 104-113 immunoglobulin heavy constant epsilon Homo sapiens 0-3 3917277-6 1985 However, MIF activity in supernatant fluid from human peripheral blood lymphocyte cultures stimulated with Con A-Sepharose was completely neutralized with MoAb anti-IFN-gamma. Sepharose 113-122 macrophage migration inhibitory factor Homo sapiens 9-12 3917277-6 1985 However, MIF activity in supernatant fluid from human peripheral blood lymphocyte cultures stimulated with Con A-Sepharose was completely neutralized with MoAb anti-IFN-gamma. Sepharose 113-122 interferon gamma Homo sapiens 165-174 3919133-7 1985 The distribution of apoA-IV in fasting and postprandial plasma was determined by 6% agarose gel chromatography. Sepharose 84-91 apolipoprotein A4 Homo sapiens 20-27 2422420-3 1985 alpha 2-Macroglobulin-plasmin complexes were purified from urokinase-activated plasma by affinity chromatography on lysine-Sepharose and gel filtration on Ultrogel AcA 22. Sepharose 123-132 plasminogen Homo sapiens 22-29 3883129-6 1985 Elution profiles of CEA, NFA-2, and NCA-2 from lectin columns, especially from concanavalin A-Sepharose columns, suggested some differences in oligosaccharide chains between them. Sepharose 94-103 CEA cell adhesion molecule 3 Homo sapiens 20-23 4059181-1 1985 Lipoprotein lipase was purified to homogeneity from bovine skim milk by a two-step procedure using chromatography on heparin-Sepharose. Sepharose 125-134 lipoprotein lipase Bos taurus 0-18 4088983-4 1985 Mouse albumin was obtained by the same purification route, but required an additional chromatography step on Cibacron Blue F3GA-agarose. Sepharose 128-135 albumin Mus musculus 6-13 2982152-6 1985 Soluble glucagon-receptor complexes adsorbed to Con A-Sepharose and could be eluted with methyl alpha-D-mannoside, indicating that the receptor molecule is a glycoprotein. Sepharose 54-63 glucagon receptor Homo sapiens 8-25 3969535-0 1985 Binding of human IgA fragments to protein A-Sepharose studied with an ELISA method. Sepharose 44-53 CD79a molecule Homo sapiens 17-20 3983905-0 1985 Separation of plasma fibronectin from associated hemagglutinating activity by elution from gelatin-agarose at pH 5.5. Sepharose 99-106 fibronectin 1 Homo sapiens 21-32 3983905-1 1985 Human plasma fibronectin prepared by elution of the adsorbed protein from gelatin-agarose by pH 5.5 citrate buffer is very low in hemagglutinating activity toward trypsinized rabbit erythrocytes, an activity previously associated with the purified plasma protein. Sepharose 82-89 fibronectin 1 Homo sapiens 13-24 2416032-4 1985 Affinity interactions between PP5 and matrices such as heparin or thrombin-Sepharose were similar and independent of the origin of PP5. Sepharose 75-84 tissue factor pathway inhibitor 2 Homo sapiens 30-33 2416032-4 1985 Affinity interactions between PP5 and matrices such as heparin or thrombin-Sepharose were similar and independent of the origin of PP5. Sepharose 75-84 coagulation factor II, thrombin Homo sapiens 66-74 6530433-1 1984 A series of affinity chromatography packings for the purification of serine and sulfhydryl esterases (acetylcholinesterase, alkaline phosphatase, urokinase and papain) have been synthesized using commercially available agarose, glass and acrylate parent matrices. Sepharose 219-226 acetylcholinesterase (Cartwright blood group) Homo sapiens 102-122 6094567-3 1984 A monoclonal antibody directed against the EGF binding site of the receptor was immobilized to Sepharose 4B as a specific immune absorbent and competitive elution with EGF was used to obtain purified EGF receptor protein with tyrosine kinase activity. Sepharose 95-107 epidermal growth factor receptor Homo sapiens 200-212 6395885-3 1984 PrP 27-30 was found in all fractions enriched for scrapie prions by discontinuous sucrose gradient sedimentation or sodium dodecyl sarcosinate-agarose gel electrophoresis. Sepharose 143-150 prion protein Mus musculus 0-9 6441597-3 1984 In addition, TPA purified to apparent homogeneity was treated with endo-beta-N-acetylglucosaminidase H (Endo-H), producing a fraction that in contrast to native TPA did not adsorb to concanavalin A-Sepharose (Con A-Sepharose). Sepharose 198-207 chromosome 20 open reading frame 181 Homo sapiens 13-16 6441597-3 1984 In addition, TPA purified to apparent homogeneity was treated with endo-beta-N-acetylglucosaminidase H (Endo-H), producing a fraction that in contrast to native TPA did not adsorb to concanavalin A-Sepharose (Con A-Sepharose). Sepharose 215-224 chromosome 20 open reading frame 181 Homo sapiens 13-16 6334580-1 1984 IgE was isolated from a patient with the hyper IgE, recurrent infection syndrome by immunoadsorption on sepharose bound goat anti-human IgE. Sepharose 104-113 immunoglobulin heavy constant epsilon Homo sapiens 47-50 6499778-2 1984 The progesterone receptor was purified to near homogeneity in the presence of 10 mM sodium molybdate by affinity chromatography on deoxycorticosterone-Sepharose followed by DEAE-Sephadex chromatography. Sepharose 151-160 progesterone receptor Homo sapiens 4-25 6335486-3 1984 The purification of IL-2 involved chromatography on Matrex Blue A sepharose and gel filtration chromatography. Sepharose 66-75 interleukin 2 Homo sapiens 20-24 6239876-5 1984 All multimers of unreduced carbohydrate-modified von Willebrand factor migrated more rapidly in SDS-agarose, but the triplet pattern of individual multimers was unchanged. Sepharose 100-107 von Willebrand factor Homo sapiens 49-70 6442290-3 1984 The monomeric 2,4-dinitrophenyl Fab"-beta-D-galactosidase conjugate was subsequently separated from unconjugated beta-D-galactosidase by affinity chromatography on a column of (anti-2,4-dinitrophenyl) IgG-Sepharose 4B. Sepharose 205-214 FA complementation group B Homo sapiens 32-35 6238094-21 1984 Additionally, during the course of these experiments, we observed that Con A-Sepharose chromatography could be used as a simple one-step method of separating BCGF from IL 2. Sepharose 77-86 interleukin 2 Homo sapiens 168-172 6333459-7 1984 Agarose electrophoresis revealed a monoclonal Ig band in the isolated IgM in all cases. Sepharose 0-7 chemokine (C-X-C motif) ligand 9 Mus musculus 46-48 6386983-5 1984 Thus, certain mouse IgG1 antibodies possess determinants in their Fab portion recognized by protein A, allowing for the purification of such Fab fragments on protein A-Sepharose. Sepharose 168-177 FA complementation group B Homo sapiens 66-69 6386983-5 1984 Thus, certain mouse IgG1 antibodies possess determinants in their Fab portion recognized by protein A, allowing for the purification of such Fab fragments on protein A-Sepharose. Sepharose 168-177 FA complementation group B Homo sapiens 141-144 6389735-2 1984 Renin was purified from Haas" preparation utilizing a pepstatin-C6-Sepharose affinity chromatography. Sepharose 67-76 renin Homo sapiens 0-5 6400370-2 1984 The antigen source was human renal renin purified from Haas" preparation by pepstatin-C6-sepharose affinity chromatography, this was used to prepare a specific human renin antibody. Sepharose 89-98 renin Homo sapiens 166-171 6390435-1 1984 The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. Sepharose 87-99 insulin Homo sapiens 49-56 6397997-5 1984 This substrate was successfully applied to monitor thiol proteinase affinity chromatography on bacitracin-Sepharose, which resulted in a 2- to 4-fold purification from commercial preparations. Sepharose 106-115 endogenous retrovirus group K member 25 Homo sapiens 57-67 6501251-4 1984 Bovine erythrocyte acetylcholinesterase released by PI-specific phospholipase C was purified by column chromatography on DEAE-cellulose, affinity gel and Sepharose 6B, to a homogeneous state, as indicated by polyacrylamide gel electrophoresis, with a recovery of 39%. Sepharose 154-163 acetylcholinesterase Equus caballus 19-39 6501251-7 1984 On elution from the Sepharose 6B column, Triton X-100-solubilized acetylcholinesterase was eluted at the void volume while the enzyme obtained by further treatment with PI-specific phospholipase C was eluted in the region corresponding to M.W. Sepharose 20-29 acetylcholinesterase Equus caballus 66-86 2408500-2 1985 2,5-Diphenyloxazole (PPO) was added to the agarose solution before pouring of the gel for electrophoresis. Sepharose 43-50 protoporphyrinogen oxidase Homo sapiens 21-24 2408500-4 1985 The radioactive detection efficiency was found to be improved over an existing procedure whereby the agarose gel was infused with PPO after electrophoresis with the aid of acetic acid. Sepharose 101-108 protoporphyrinogen oxidase Homo sapiens 130-133 2982330-1 1985 NAD kinase from human neutrophils has been partially purified by sequential application of Red Agarose, ion-exchange, and gel-filtration chromatography. Sepharose 95-102 NAD kinase Homo sapiens 0-10 6150036-11 1984 Caldesmon prepared by calmodulin-Sepharose affinity chromatography was contaminated with caldesmon kinase activity and was unable to inhibit actomyosin ATPase activity or superprecipitation. Sepharose 33-42 caldesmon 1 Gallus gallus 0-9 6150036-11 1984 Caldesmon prepared by calmodulin-Sepharose affinity chromatography was contaminated with caldesmon kinase activity and was unable to inhibit actomyosin ATPase activity or superprecipitation. Sepharose 33-42 calmodulin 2 Gallus gallus 22-32 6209271-3 1984 The antibodies were purified by affinity chromatography in EDTA on columns of prothrombin-Sepharose. Sepharose 90-99 coagulation factor II, thrombin Homo sapiens 78-89 6508794-2 1984 Agarose gel electrophoresis revealed that the only detectable CK isoenzyme present was CK-BB, in contrast to the human serum isoenzyme which was CK-MM. Sepharose 0-7 creatine kinase B Rattus norvegicus 87-92 6542365-2 1984 Immunoreactive ANF has been extracted from rat plasma by immunoaffinity column on Sepharose-4B anti-ANF or by Vycor glass. Sepharose 82-91 natriuretic peptide A Rattus norvegicus 15-18 6542365-3 1984 The mean concentrations of IR-ANF in ether anesthetized rats were found to be 1.61 +/- 0.14 ng/ml in female and 1.25 +/- 0.21 ng/ml in male rats when extracted on Sepharose-4B anti-ANF, and 1.21 +/- 0.10 ng/ml in females and 1.02 +/- 0.11 ng/ml in males when extracted by Vycor glass. Sepharose 163-172 natriuretic peptide A Rattus norvegicus 30-33 6335631-0 1984 Affinity chromatography of alpha-1-protease inhibitor using Sepharose-4B-bound anhydrochymotrypsin. Sepharose 60-72 serpin family A member 1 Homo sapiens 27-53 6335631-1 1984 Sepharose 4B-bound bovine anhydrochymotrypsin (AnhCT), a catalytically inactive form of chymotrypsin, was shown to be effective for retaining active alpha-1-protease inhibitor (alpha-1-PI, also alpha-1-antitrypsin) from human plasma, while showing no measurable affinity for oxidized or protease complexed alpha-1-PI, or for most other plasma proteins. Sepharose 0-12 serpin family A member 1 Homo sapiens 149-175 6335631-1 1984 Sepharose 4B-bound bovine anhydrochymotrypsin (AnhCT), a catalytically inactive form of chymotrypsin, was shown to be effective for retaining active alpha-1-protease inhibitor (alpha-1-PI, also alpha-1-antitrypsin) from human plasma, while showing no measurable affinity for oxidized or protease complexed alpha-1-PI, or for most other plasma proteins. Sepharose 0-12 serpin family A member 1 Homo sapiens 177-187 6335631-1 1984 Sepharose 4B-bound bovine anhydrochymotrypsin (AnhCT), a catalytically inactive form of chymotrypsin, was shown to be effective for retaining active alpha-1-protease inhibitor (alpha-1-PI, also alpha-1-antitrypsin) from human plasma, while showing no measurable affinity for oxidized or protease complexed alpha-1-PI, or for most other plasma proteins. Sepharose 0-12 serpin family A member 1 Bos taurus 194-213 6335631-1 1984 Sepharose 4B-bound bovine anhydrochymotrypsin (AnhCT), a catalytically inactive form of chymotrypsin, was shown to be effective for retaining active alpha-1-protease inhibitor (alpha-1-PI, also alpha-1-antitrypsin) from human plasma, while showing no measurable affinity for oxidized or protease complexed alpha-1-PI, or for most other plasma proteins. Sepharose 0-12 serpin family A member 1 Homo sapiens 306-316 6335631-5 1984 Therefore, an AnhCT-Sepharose 4B resin has been demonstrated to be of value for isolating active forms of alpha-1-PI from solutions, and may also be useful for the isolation of inter-alpha-trypsin inhibitor. Sepharose 20-29 serpin family A member 1 Homo sapiens 106-116 6441307-0 1984 Specific fibrinogen quantitation by electroimmunodiffusion in agarose gel containing heparin. Sepharose 62-69 fibrinogen beta chain Homo sapiens 9-19 6441307-1 1984 In the determination of plasma fibrinogen by electroimmunodiffusion, commercial sodium heparin, previously included in the agarose gel, by interacting with fibrinogen molecules, enhances their anodic mobility more strongly than after carbamylation with potassium cyanate. Sepharose 123-130 fibrinogen beta chain Homo sapiens 156-166 6489349-2 1984 Thrombospondin synthesized and secreted by human endothelial cells in culture binds specifically to fibronectin immobilized on Sepharose beads. Sepharose 127-136 fibronectin 1 Homo sapiens 100-111 6237688-6 1984 A partial fractionation of the antibody into two groups showing differential reactivities toward opsin and rhodopsin was achieved by affinity chromatography on opsin-Sepharose. Sepharose 166-175 rhodopsin Bos taurus 107-116 6386053-1 1984 Two forms of an acid proteinase have been purified from human erythrocyte membranes by a simple method involving selective extraction with 0.5% Brij 35, affinity chromatography on pepstatin A-Sepharose 4B, and chromatography on Sephacryl S-200 and DEAE-Sephadex G-100. Sepharose 192-204 endogenous retrovirus group K member 25 Homo sapiens 21-31 6435684-4 1984 A high-molecular-weight t-PA form comigrating with a t-PA-inhibitor complex (Mr 95 000-135 000) from cultured human endothelial cells was purified from plasma by immunoadsorption on anti-t-PA-Sepharose followed by gel filtration on Sephadex G-150. Sepharose 192-201 plasminogen activator, tissue type Homo sapiens 24-28 6509026-1 1984 When rat liver cytosol containing [3H]dexamethasone-glucocorticoid receptor complex is exposed to immobilized heparin (Sepharose-heparin; Seph-hep) the steroid receptor complex binds to the substituted Sepharose avidly [Kd = 3.5 (+/- 1.7) X 10(-10) M], and 80-90% of the receptor present is adsorbed to the solid phase after 40 min at 0 degree C. The binding is enhanced by Mn2+ (10 mM) and Mg2+, whereas Ca2+ and Sr2+ are ineffective. Sepharose 119-128 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 52-75 6509026-1 1984 When rat liver cytosol containing [3H]dexamethasone-glucocorticoid receptor complex is exposed to immobilized heparin (Sepharose-heparin; Seph-hep) the steroid receptor complex binds to the substituted Sepharose avidly [Kd = 3.5 (+/- 1.7) X 10(-10) M], and 80-90% of the receptor present is adsorbed to the solid phase after 40 min at 0 degree C. The binding is enhanced by Mn2+ (10 mM) and Mg2+, whereas Ca2+ and Sr2+ are ineffective. Sepharose 202-211 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 52-75 6442106-1 1984 Human interleukin 2, produced by phytohemagglutinin-stimulated peripheral blood lymphocytes cultured in the absence of serum, has been purified to apparent homogeneity with a two-step combination of affinity chromatography with Cibacron blue-Sepharose and gel filtration. Sepharose 242-251 interleukin 2 Homo sapiens 6-19 6497381-1 1984 Adenylate cyclase from rat kidney membranes solubilized with Lubrol-PX, was resolved into calmodulin-insensitive and calmodulin-sensitive forms using DEAE-Sephacel and calmodulin-Sepharose affinity chromatography. Sepharose 179-188 calmodulin 1 Rattus norvegicus 117-127 6497381-1 1984 Adenylate cyclase from rat kidney membranes solubilized with Lubrol-PX, was resolved into calmodulin-insensitive and calmodulin-sensitive forms using DEAE-Sephacel and calmodulin-Sepharose affinity chromatography. Sepharose 179-188 calmodulin 1 Rattus norvegicus 117-127 6508740-0 1984 Endogenous ligands of rat lung beta-galactoside-binding protein (galaptin) isolated by affinity chromatography on carboxyamidomethylated-galaptin-Sepharose. Sepharose 146-155 galectin 1 Rattus norvegicus 31-63 6508740-0 1984 Endogenous ligands of rat lung beta-galactoside-binding protein (galaptin) isolated by affinity chromatography on carboxyamidomethylated-galaptin-Sepharose. Sepharose 146-155 galectin 1 Rattus norvegicus 65-73 6508740-0 1984 Endogenous ligands of rat lung beta-galactoside-binding protein (galaptin) isolated by affinity chromatography on carboxyamidomethylated-galaptin-Sepharose. Sepharose 146-155 galectin 1 Rattus norvegicus 137-145 6508740-3 1984 Reaction of rat lung galaptin with iodoacetamide resulted in a stable active carboxyamidomethylated galaptin that could be coupled to Sepharose. Sepharose 134-143 galectin 1 Rattus norvegicus 21-29 6508740-3 1984 Reaction of rat lung galaptin with iodoacetamide resulted in a stable active carboxyamidomethylated galaptin that could be coupled to Sepharose. Sepharose 134-143 galectin 1 Rattus norvegicus 100-108 6508740-5 1984 The carboxyamidomethylated-galaptin-Sepharose affinity matrix was used to search for endogenous ligands in 13-day-rat lung. Sepharose 36-45 galectin 1 Rattus norvegicus 27-35 6207956-3 1984 In a more detailed analysis of three subjects, we measured the lipoprotein association of apo E by column chromatography on agarose beads, before and after its precipitation from plasma. Sepharose 124-131 apolipoprotein E Homo sapiens 90-95 6386441-5 1984 Active and latent renin showed strong affinity to an antirat renin immunoglobulin-Sepharose column, indicating a close immunological relationship of latent renin to active renin. Sepharose 82-91 renin Rattus norvegicus 18-23 6386441-5 1984 Active and latent renin showed strong affinity to an antirat renin immunoglobulin-Sepharose column, indicating a close immunological relationship of latent renin to active renin. Sepharose 82-91 renin Rattus norvegicus 61-66 6386441-5 1984 Active and latent renin showed strong affinity to an antirat renin immunoglobulin-Sepharose column, indicating a close immunological relationship of latent renin to active renin. Sepharose 82-91 renin Rattus norvegicus 61-66 6386441-5 1984 Active and latent renin showed strong affinity to an antirat renin immunoglobulin-Sepharose column, indicating a close immunological relationship of latent renin to active renin. Sepharose 82-91 renin Rattus norvegicus 61-66 6334100-5 1984 The C3bR was isolated either by immunoprecipitation with anti-C3bR antibodies or by affinity chromatography with hemolytically inactive components in which the internal thioester bond within the alpha-chain was cleaved (iC3)- or iC4-Sepharose. Sepharose 233-242 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 4-8 6526819-4 1984 Immunoadsorption on a column of the monoclonal antibody-Sepharose 4B was shown to be useful for the removal of ornithine decarboxylase from antizyme inhibitor preparations, an essential procedure for the accurate assay of either ornithine decarboxylase-antizyme complex or antizyme inhibitor. Sepharose 56-68 ornithine decarboxylase 1 Rattus norvegicus 111-134 6526819-4 1984 Immunoadsorption on a column of the monoclonal antibody-Sepharose 4B was shown to be useful for the removal of ornithine decarboxylase from antizyme inhibitor preparations, an essential procedure for the accurate assay of either ornithine decarboxylase-antizyme complex or antizyme inhibitor. Sepharose 56-68 ornithine decarboxylase 1 Rattus norvegicus 229-252 6568228-10 1984 Again in serum-free media, neutrophils degraded eight-fold more elastin than macrophages but only macrophages degraded sepharose-coupled elastin in the presence of 10% serum. Sepharose 119-128 elastin Homo sapiens 137-144 6395434-1 1984 A technique is described to completely remove antithrombin III (AT) from small amounts of human plasma by immunoaffinity chromatography on antibodies against human AT linked to Sepharose 4B. Sepharose 177-186 serpin family C member 1 Homo sapiens 46-62 6395434-1 1984 A technique is described to completely remove antithrombin III (AT) from small amounts of human plasma by immunoaffinity chromatography on antibodies against human AT linked to Sepharose 4B. Sepharose 177-186 serpin family C member 1 Homo sapiens 64-66 6395434-1 1984 A technique is described to completely remove antithrombin III (AT) from small amounts of human plasma by immunoaffinity chromatography on antibodies against human AT linked to Sepharose 4B. Sepharose 177-186 serpin family C member 1 Homo sapiens 164-166 6487647-3 1984 Rat HDL was isolated at d 1.085-1.21 g/ml and apolipoprotein E-free HDL was prepared by heparin Sepharose chromatography. Sepharose 96-105 apolipoprotein E Rattus norvegicus 46-62 6386184-10 1984 Coupling of bovine brain mixed gangliosides to agarose resulted in a matrix capable of reversibly binding MIF. Sepharose 47-54 macrophage migration inhibitory factor Bos taurus 106-109 6497854-6 1984 L-type enzyme was bound to Sepharose-immobilized Procion Blue MX-R specifically in the presence of Mg2+, whereas binding of the M-type enzyme was Mg2+-independent. Sepharose 27-36 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 62-66 6090461-5 1984 Thrombomodulin was then isolated by affinity chromatography on a column of thrombin-Sepharose wherein the thrombin had been previously inactivated with diisopropyl fluorophosphate. Sepharose 84-93 thrombomodulin Homo sapiens 0-14 6090461-5 1984 Thrombomodulin was then isolated by affinity chromatography on a column of thrombin-Sepharose wherein the thrombin had been previously inactivated with diisopropyl fluorophosphate. Sepharose 84-93 coagulation factor II, thrombin Homo sapiens 75-83 6541221-4 1984 When coupled to agarose, the Erythrina inhibitor provided an effective reagent for affinity purification of tissue plasminogen activator from melanoma cell-conditioned tissue culture medium. Sepharose 16-23 chromosome 20 open reading frame 181 Homo sapiens 108-136 6089906-1 1984 Arachidonate 5-lipoxygenase was partially purified from rat basophilic leukemia cells with the aid of ATP as a ligand linked to Sepharose. Sepharose 128-137 arachidonate 5-lipoxygenase Rattus norvegicus 0-27 6388206-1 1984 A radioimmunoassay has been developed for IGF-II, using Sepharose-coupled antibodies. Sepharose 56-65 insulin like growth factor 2 Homo sapiens 42-48 6393989-1 1984 Yeast glyceraldehyde-3-phosphate dehydrogenase (GPDH) covalently attached to CNBr-activated Sepharose 4B was shown to be capable of binding soluble yeast phosphoglycerate kinase (PGK) in the course of incubation in the presence of an excess of 1,3-diphosphoglycerate. Sepharose 92-104 phosphoglycerate kinase Saccharomyces cerevisiae S288C 154-177 6393989-1 1984 Yeast glyceraldehyde-3-phosphate dehydrogenase (GPDH) covalently attached to CNBr-activated Sepharose 4B was shown to be capable of binding soluble yeast phosphoglycerate kinase (PGK) in the course of incubation in the presence of an excess of 1,3-diphosphoglycerate. Sepharose 92-104 phosphoglycerate kinase Saccharomyces cerevisiae S288C 179-182 6334644-7 1984 Soluble or Sepharose-bound anti-Ti monoclonal antibodies, like physiologic ligand (antigen/MHC), enhanced proliferative responses to purified IL-2 by inducing a 6-fold increase in surface IL-2 receptor expression. Sepharose 11-20 major histocompatibility complex, class I, C Homo sapiens 91-94 6334644-7 1984 Soluble or Sepharose-bound anti-Ti monoclonal antibodies, like physiologic ligand (antigen/MHC), enhanced proliferative responses to purified IL-2 by inducing a 6-fold increase in surface IL-2 receptor expression. Sepharose 11-20 interleukin 2 Homo sapiens 142-146 6334644-7 1984 Soluble or Sepharose-bound anti-Ti monoclonal antibodies, like physiologic ligand (antigen/MHC), enhanced proliferative responses to purified IL-2 by inducing a 6-fold increase in surface IL-2 receptor expression. Sepharose 11-20 interleukin 2 receptor subunit beta Homo sapiens 188-201 6334644-8 1984 In contrast, only Sepharose-bound anti-Ti or physiologic ligand triggered endogenous clonal IL-2 production and resulted in subsequent proliferation. Sepharose 18-27 interleukin 2 Homo sapiens 92-96 6520123-1 1984 Spermidine synthase was purified to homogeneity from rat and pig liver by a method modified from a previously reported one using DEAE-Sepharose, S-adenosyl(5")-3-thiopropylamine-Sepharose affinity chromatography, Sephacryl S-300 gel filtration and polyacrylamide gel electrophoresis. Sepharose 134-143 spermidine synthase Rattus norvegicus 0-19 6520125-1 1984 Porcine pancreatic phospholipase A2 (PLA2) was immobilized to Sepharose 4B and porcine serum was passed through this affinity column. Sepharose 62-74 phospholipase A2 group IB Homo sapiens 19-35 6520125-1 1984 Porcine pancreatic phospholipase A2 (PLA2) was immobilized to Sepharose 4B and porcine serum was passed through this affinity column. Sepharose 62-74 phospholipase A2 group IB Homo sapiens 37-41 6480826-5 1984 The new series of apo E components, named apo E-Suita, was identical with the ordinary apo E in its interaction with heparin-Sepharose gel and with anti-apo E antibody. Sepharose 125-134 apolipoprotein E Homo sapiens 18-23 6480826-5 1984 The new series of apo E components, named apo E-Suita, was identical with the ordinary apo E in its interaction with heparin-Sepharose gel and with anti-apo E antibody. Sepharose 125-134 apolipoprotein E Homo sapiens 42-47 6480826-5 1984 The new series of apo E components, named apo E-Suita, was identical with the ordinary apo E in its interaction with heparin-Sepharose gel and with anti-apo E antibody. Sepharose 125-134 apolipoprotein E Homo sapiens 42-47 6480826-5 1984 The new series of apo E components, named apo E-Suita, was identical with the ordinary apo E in its interaction with heparin-Sepharose gel and with anti-apo E antibody. Sepharose 125-134 apolipoprotein E Homo sapiens 42-47 6083471-1 1984 Two different kinds of immunosorbents were prepared that contained the synthetic myelin basic protein didecapeptide S82 (TTHYGSLPQKAQGHRDQDEG)--one coupled with AH-Sepharose 4B through hexanoate spacers to the C-terminal glycyl residue; the other, with CH-Sepharose 4B through hexanoate spacers to the N-terminal threonine residue. Sepharose 164-173 myelin basic protein Homo sapiens 81-101 6083471-1 1984 Two different kinds of immunosorbents were prepared that contained the synthetic myelin basic protein didecapeptide S82 (TTHYGSLPQKAQGHRDQDEG)--one coupled with AH-Sepharose 4B through hexanoate spacers to the C-terminal glycyl residue; the other, with CH-Sepharose 4B through hexanoate spacers to the N-terminal threonine residue. Sepharose 256-265 myelin basic protein Homo sapiens 81-101 6389730-2 1984 The post-proline endopeptidase was able to be completely separated from dipeptidyl peptidase IV (EC 3.4.14.5) by hydrophobic phenyl Sepharose chromatography. Sepharose 132-141 dipeptidyl peptidase 4 Homo sapiens 72-95 6383479-1 1984 A polypeptide proteinase inhibitor from human articular cartilage has been purified to homogeneity by stepwise Sephadex G-75, heparin-Sepharose and octyl-Sepharose affinity chromatography. Sepharose 134-143 endogenous retrovirus group K member 25 Homo sapiens 14-24 6479161-10 1984 IEF on agarose of either HDL2 or HDL3 allowed us to collect eight different fractions, which have the same pI in either lipoprotein class. Sepharose 7-14 junctophilin 3 Homo sapiens 25-29 6540777-2 1984 The alpha(1,3)fucosyltransferase in LEC12 cell extracts binds to GDP-hexanolamine-Sepharose and is unaffected by 3 mM N-ethylmaleimide. Sepharose 82-91 alpha(1,3)fucosyltransferase Cricetulus griseus 4-32 6205900-3 1984 The enzyme was quantitatively and specifically adsorbed to beads of anti-lactoperoxidase (bovine)-Protein A-Sepharose. Sepharose 108-117 lactoperoxidase Bos taurus 73-88 6432075-2 1984 All the multimers of vWF:Ag could be seen in the 1.6% SDS-agarose gel electrophoresis patterns of plasma and platelet lysates. Sepharose 58-65 von Willebrand factor Homo sapiens 21-24 6088369-4 1984 The binding affinity for labeled hCG was investigated by polyethylene glycol precipitation, by complex formation with protein A-Sepharose, and by gel filtration. Sepharose 128-137 chorionic gonadotropin subunit beta 5 Homo sapiens 33-36 6594315-9 1984 The solubilized sulfatase strongly bound to ConA-Sepharose. Sepharose 49-58 arylsulfatase family member H Homo sapiens 16-25 6086701-7 1984 When the HMW neurophysin was incubated with trypsin, most of the immunoreactivity was converted into a smaller neurophysin which bound to a vasopressin-agarose column in a pH-dependent manner. Sepharose 152-159 arginine vasopressin Homo sapiens 140-151 6086701-9 1984 This peak coeluted with synthetic arginine vasopressin on gel filtration and had the characteristic affinity of vasopressin for neurophysin-agarose. Sepharose 140-147 arginine vasopressin Homo sapiens 112-123 6088554-8 1984 Solubilization of affinity-labeled membranes in Nonidet P-40 followed by affinity chromatography on wheat germ agglutinin-agarose revealed that all three CCK-binding proteins specifically interact with this lectin and can be eluted with N-acetyl-D-glucosamine. Sepharose 122-129 cholecystokinin Rattus norvegicus 154-157 6094522-5 1984 Furthermore, PP1 enhanced ATP-agarose binding of the "activated" complex, indicating that the PP1 binding site(s) on the receptor is different from the ATP binding site(s). Sepharose 30-37 neuropeptide Y receptor Y4 Rattus norvegicus 13-16 6094522-5 1984 Furthermore, PP1 enhanced ATP-agarose binding of the "activated" complex, indicating that the PP1 binding site(s) on the receptor is different from the ATP binding site(s). Sepharose 30-37 neuropeptide Y receptor Y4 Rattus norvegicus 94-97 6381509-6 1984 When the melanoma cells were solubilized in 1% Triton X-100 and the insulin receptor was partially purified by chromatography on wheat germ agglutinin-agarose, a similar pattern of binding was observed. Sepharose 151-158 insulin receptor Mus musculus 68-84 6501264-1 1984 Phospholipase A2 was purified from rat pancreas by heat treatment of the homogenate and the sequential use of DEAE-Sepharose chromatography, CM-Sepharose chromatography, and reverse-phase high-performance liquid chromatography (HPLC). Sepharose 115-124 phospholipase A2 group IB Rattus norvegicus 0-16 6501264-1 1984 Phospholipase A2 was purified from rat pancreas by heat treatment of the homogenate and the sequential use of DEAE-Sepharose chromatography, CM-Sepharose chromatography, and reverse-phase high-performance liquid chromatography (HPLC). Sepharose 144-153 phospholipase A2 group IB Rattus norvegicus 0-16 6501264-2 1984 Prophospholipase A2 was not separated from the phospholipase A2 by CM-Sepharose chromatography under the conditions used, but it was well resolved by the reverse-phase HPLC. Sepharose 70-79 phospholipase A2 group IB Rattus norvegicus 3-19 6493223-2 1984 Further purification on Dicap-MP sepharose 4B, a choline analog compound, led to ACHR preparations with specific activities of 2-7 nmol/mg protein. Sepharose 33-42 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 81-85 6484483-5 1984 Addition of the serine protease inhibitors DFP and benzamidine, o-phenanthroline, and iodoacetamide substantially retarded the proteolytic alterations of the proteins as demonstrated by both agarose electrophoresis and SDS-PAGE. Sepharose 191-198 coagulation factor II, thrombin Homo sapiens 16-31 6541374-0 1984 A sensitive assay for tissue plasminogen activator activity in plasma, using adsorption on lysine-sepharose. Sepharose 98-107 chromosome 20 open reading frame 181 Homo sapiens 22-50 6541374-1 1984 Tissue plasminogen activator (t-PA) in plasma was separated from inhibitors by adsorption on lysine-Sepharose. Sepharose 100-109 plasminogen activator, tissue type Homo sapiens 0-34 6466686-14 1984 Schiff"s staining on polyacrylamide gel and interaction with Con A-Sepharose indicate that trehalase is a glycoprotein. Sepharose 67-76 trehalase Oryctolagus cuniculus 91-100 6547949-6 1984 3) Inactivation by the reagent yielded calmodulin that was no longer retained on a phenothiazine-Sepharose column under conditions in which unreacted calmodulin was retained. Sepharose 97-106 calmodulin 1 Homo sapiens 39-49 6235859-1 1984 Employing agarose gel electrophoresis, physiological concentrations of plasmin have been shown to degrade purified proteoglycan monomers and aggregates isolated from bovine articular cartilage. Sepharose 10-17 plasminogen Bos taurus 71-78 6477573-2 1984 A beta-galactoside-binding lectin was extracted from human placenta homogenate with lactose solution and purified to apparent homogeneity by affinity chromatography on asialofetuin-Sepharose. Sepharose 181-190 galectin 16 Homo sapiens 2-33 6086661-4 1984 Transferrin receptors from uninduced and differentiating cells were partially purified by affinity chromatography on transferrin-Sepharose and shown to be disulfide-bridged homodimers of a polypeptide with an apparent molecular weight of approximately 90,000. Sepharose 129-138 transferrin Homo sapiens 0-11 6378914-1 1984 The characteristics of the insulin- and epidermal growth factor (EGF)-stimulated tyrosine-specific protein kinases in a wheat germ lectin-Sepharose-purified preparation of solubilized placenta membranes were compared. Sepharose 138-147 epidermal growth factor Homo sapiens 65-68 6382164-3 1984 Molecules containing large complementary inserts are annealed by recA protein to form large multimeric aggregates that migrate slowly in agarose gels. Sepharose 137-144 RAD51 recombinase Homo sapiens 65-69 6467619-1 1984 Isoelectric focusing in agarose gel has been used for separation of different molecular forms of transferrin (Tf) in sera and plasma from alcoholics and controls. Sepharose 24-31 transferrin Homo sapiens 97-108 6467619-1 1984 Isoelectric focusing in agarose gel has been used for separation of different molecular forms of transferrin (Tf) in sera and plasma from alcoholics and controls. Sepharose 24-31 transferrin Homo sapiens 110-112 6208755-1 1984 A combination of agarose gel electrophoresis and a newly developed technique of electro-affinity transfer was applied to the detection of circulating immune complexes of human alpha-fetoprotein (AFP) and anti-AFP. Sepharose 17-24 alpha fetoprotein Homo sapiens 176-193 6208755-1 1984 A combination of agarose gel electrophoresis and a newly developed technique of electro-affinity transfer was applied to the detection of circulating immune complexes of human alpha-fetoprotein (AFP) and anti-AFP. Sepharose 17-24 alpha fetoprotein Homo sapiens 195-198 6208755-3 1984 Artificial complexes formed in vitro from human AFP and rabbit anti-AFP were clearly separated from free AFP by the agarose electrophoresis. Sepharose 116-123 alpha fetoprotein Homo sapiens 48-51 6208755-3 1984 Artificial complexes formed in vitro from human AFP and rabbit anti-AFP were clearly separated from free AFP by the agarose electrophoresis. Sepharose 116-123 alpha fetoprotein Homo sapiens 68-71 6208755-3 1984 Artificial complexes formed in vitro from human AFP and rabbit anti-AFP were clearly separated from free AFP by the agarose electrophoresis. Sepharose 116-123 alpha fetoprotein Homo sapiens 68-71 6333832-0 1984 Purification of patient-related allergens by means of bioaffinity chromatography on a sepharose anti-IgE patient IgE immunosorbent. Sepharose 86-95 immunoglobulin heavy constant epsilon Homo sapiens 101-104 6333832-0 1984 Purification of patient-related allergens by means of bioaffinity chromatography on a sepharose anti-IgE patient IgE immunosorbent. Sepharose 86-95 immunoglobulin heavy constant epsilon Homo sapiens 113-116 6333832-1 1984 A method is described for bioaffinity chromatographic purification of patient-specific IgE reactive allergens by using sepharose coupled rabbit anti-human IgE antibodies, patient serum and an allergen extract. Sepharose 119-128 immunoglobulin heavy constant epsilon Homo sapiens 87-90 6333832-1 1984 A method is described for bioaffinity chromatographic purification of patient-specific IgE reactive allergens by using sepharose coupled rabbit anti-human IgE antibodies, patient serum and an allergen extract. Sepharose 119-128 immunoglobulin heavy constant epsilon Homo sapiens 155-158 6466396-5 1984 All normal and pathologic synovial fluid fibronectins showed a remarkably lower electrophoretic mobility compared with that of plasma fibronectin, when separated according to net molecular charge on agarose gel. Sepharose 199-206 fibronectin 1 Homo sapiens 41-52 6478585-3 1984 Underestimation of CK-MM occurs because the enzyme reagent are diluted approximately five-fold by the agarose, which results in the non-linear development of enzyme activity under sub-optimal conditions. Sepharose 102-109 creatine kinase, M-type Homo sapiens 19-24 6490356-7 1984 Calmodulin was isolated from NK-enriched populations by affinity chromatography on sepharose-fluphenazine columns. Sepharose 83-92 calmodulin 2 Mus musculus 0-10 6470622-1 1984 Sexual dimorphism of mouse complement component 5 (C5) was detected by isoelectric focusing of desialated ethylenediamine tetraacetic acid (EDTA)-plasma on agarose gel, followed by immunofixation with anti-mouse C5. Sepharose 156-163 hemolytic complement Mus musculus 51-53 6236290-2 1984 It has been purified 80- to 160-fold by solubilization with Triton X-100 and affinity chromatography on a calmodulin-Sepharose 4B column. Sepharose 117-126 calmodulin 1 Rattus norvegicus 106-116 6747440-3 1984 Antithrombin III from two members of this family was purified by dextran sulfate precipitation, affinity chromatography on heparin-Sepharose, and ion-exchange chromatography on DEAE-Sephadex A-50. Sepharose 131-140 serpin family C member 1 Homo sapiens 0-16 6747440-7 1984 This was shown by crossed immunoelectrophoresis using heparin in the first dimension, by the elution pattern during salt gradient elution of antithrombin III from heparin-Sepharose, and by heparin enhancement of intrinsic fluorescence. Sepharose 171-180 serpin family C member 1 Homo sapiens 141-157 6389954-4 1984 Electrophoresis analysis of proteinuria in agarose gel and sodium dodecyl sulfate polyacrylamide gel showed that injection of saturating doses of beta 2m induced the excretion of proteins of similar size but different charge and that of other proteins of different size. Sepharose 43-50 beta-2-microglobulin Homo sapiens 146-153 6390435-1 1984 The ligand N alpha, B1-(6-biotinylamido)hexanoyl-insulin was attached noncovalently to Sepharose 4B immobilized succinoylavidin to form an insulin-affinity resin. Sepharose 87-99 insulin Homo sapiens 139-146 6591188-2 1984 Immunoblotting of a standard antigen preparation from either serum or ascites fluid after electrophoresis in 1% agarose showed that the specific DU-PAN-2 activity resided in two major high molecular weight bands. Sepharose 112-119 poly(A) specific ribonuclease subunit PAN2 Homo sapiens 148-153 6480737-5 1984 A comparison of the elution profiles of rhodopsin purified by this method with that purified by Concanavalin A-Sepharose 4B affinity chromatography suggested that rhodopsin from high-performance chromatography was slightly purer than the conventionally purified rhodopsin. Sepharose 111-123 rhodopsin Bos taurus 163-172 6480737-5 1984 A comparison of the elution profiles of rhodopsin purified by this method with that purified by Concanavalin A-Sepharose 4B affinity chromatography suggested that rhodopsin from high-performance chromatography was slightly purer than the conventionally purified rhodopsin. Sepharose 111-123 rhodopsin Bos taurus 163-172 6234362-5 1984 The 43,000 Mr Fc gamma receptor of the eosinophil and the 31,000 and 34,000 Mr fragments that appear to be derived from it were able to rebind selectively to human IgG1-Sepharose, Fc gamma 1-Sepharose, IgG3-Sepharose, and Fc gamma 3-Sepharose. Sepharose 169-178 Fc gamma receptor Ia Homo sapiens 14-31 6234362-5 1984 The 43,000 Mr Fc gamma receptor of the eosinophil and the 31,000 and 34,000 Mr fragments that appear to be derived from it were able to rebind selectively to human IgG1-Sepharose, Fc gamma 1-Sepharose, IgG3-Sepharose, and Fc gamma 3-Sepharose. Sepharose 191-200 Fc gamma receptor Ia Homo sapiens 14-31 6234362-5 1984 The 43,000 Mr Fc gamma receptor of the eosinophil and the 31,000 and 34,000 Mr fragments that appear to be derived from it were able to rebind selectively to human IgG1-Sepharose, Fc gamma 1-Sepharose, IgG3-Sepharose, and Fc gamma 3-Sepharose. Sepharose 191-200 Fc gamma receptor Ia Homo sapiens 14-31 6234362-5 1984 The 43,000 Mr Fc gamma receptor of the eosinophil and the 31,000 and 34,000 Mr fragments that appear to be derived from it were able to rebind selectively to human IgG1-Sepharose, Fc gamma 1-Sepharose, IgG3-Sepharose, and Fc gamma 3-Sepharose. Sepharose 191-200 Fc gamma receptor Ia Homo sapiens 14-31 6234362-6 1984 In contrast, the 52,000 to 68,000 Mr Fc gamma receptor from neutrophils could rebind only to IgG1-Sepharose and Fc gamma 1-Sepharose. Sepharose 98-107 Fc gamma receptor Ia Homo sapiens 37-54 6234362-6 1984 In contrast, the 52,000 to 68,000 Mr Fc gamma receptor from neutrophils could rebind only to IgG1-Sepharose and Fc gamma 1-Sepharose. Sepharose 123-132 Fc gamma receptor Ia Homo sapiens 37-54 6330113-5 1984 The 32P content of the EGF receptor purified with EGF-Sepharose was increased by 38% compared with the same amount of receptor isolated from control cells. Sepharose 54-63 epidermal growth factor Homo sapiens 23-26 6331319-8 1984 Formation of a Ca2+-dependent complex between calmodulin and the phosphatase was demonstrated by a calmodulin-Sepharose affinity column, gel-filtration chromatography, and sedimentation on a sucrose density gradient. Sepharose 110-119 calmodulin Bos taurus 46-56 6331319-8 1984 Formation of a Ca2+-dependent complex between calmodulin and the phosphatase was demonstrated by a calmodulin-Sepharose affinity column, gel-filtration chromatography, and sedimentation on a sucrose density gradient. Sepharose 110-119 calmodulin Bos taurus 99-109 6330113-5 1984 The 32P content of the EGF receptor purified with EGF-Sepharose was increased by 38% compared with the same amount of receptor isolated from control cells. Sepharose 54-63 epidermal growth factor Homo sapiens 50-53 6086158-3 1984 This protein cleaved pBR 322 DNA and the cleavage products showed the presence of a major band corresponding to the circular form of the DNA when analysed in agarose gels under non-denaturing conditions. Sepharose 158-165 translocator protein Rattus norvegicus 21-24 6504047-5 1984 Fractionation of extracts of mouse lung on affinity columns of asialofetuin-Sepharose yielded a protein whose molecular weight, carbohydrate-binding specificity, and immunological properties suggest that it is CBP35 derived from the lung, hereafter designated CBP35 (lung). Sepharose 76-85 lectin, galactose binding, soluble 3 Mus musculus 210-215 6238856-0 1984 Attachment and phagocytosis by salmon macrophages of agarose beads coated with human C3b and C3bi. Sepharose 53-60 endogenous retrovirus group K member 3 Homo sapiens 85-88 6238856-3 1984 Furthermore, agarose beads coated with human C3b and C3bi after incubation with isolated complement factors (C3, D, B), were associated to 30-40% of the phagocytes. Sepharose 13-20 endogenous retrovirus group K member 3 Homo sapiens 45-48 6238856-5 1984 Conversion by trypsin treatment (0.01%) of agarose bound C3bi to C3d, abolished the association of such beads to the macrophages. Sepharose 43-50 endogenous retrovirus group K member 13 Homo sapiens 65-68 6238856-7 1984 Agarose beads coated with these ligands (C3b and C3bi) are attached and ingested by the phagocytes. Sepharose 0-7 endogenous retrovirus group K member 3 Homo sapiens 41-44 6208186-6 1984 PRF was purified from the "lysine-Sepharose eluate" by DEAE-cellulose column chromatography and gel filtration on a Sephadex G-100 column. Sepharose 34-43 Spi-C transcription factor (Spi-1/PU.1 related) Mus musculus 0-3 6588385-3 1984 To show that significant alterations can also occur in vivo, antibodies to Xenopus N-CAM were embedded in agarose microcylinders and implanted in the tecta of juvenile Xenopus laevis frogs that were undergoing regeneration of their retinotectal projections; 1 week later, the effects of implantation on the projection pattern from the optic nerve were determined. Sepharose 106-113 neural cell adhesion molecule 1 L homeolog Xenopus laevis 83-88 6379853-1 1984 We have examined to what extent human fibronectin associated with agarose beads with a 5- to 10-micron diameter mediates binding and uptake of the beads by mouse macrophages and human monocytes. Sepharose 66-73 fibronectin 1 Homo sapiens 38-49 6379853-2 1984 Native agarose beads preincubated with 125I-fibronectin were neither associated with nor taken up by mouse macrophages after 30 min of incubation under serum-free conditions. Sepharose 7-14 fibronectin 1 Mus musculus 44-55 6379853-3 1984 When fibronectin was cross-linked to cyanogen bromide-activated agarose beads or incubated with gelatinized beads, this resulted in a significant increase in particle binding by macrophages and monocytes as compared with gelatinized beads, whereas the fraction of cells with ingested particles remained unaltered. Sepharose 64-71 fibronectin 1 Homo sapiens 5-16 6379853-6 1984 Since agarose beads are activators of the alternative pathway of complement, and fibronectin is reported to bind to factor C3, we speculate that cell-derived C3b is bound to the beads and fibronectin-coated beads are ingested by the phagocytes via complement C3b receptors on the cells. Sepharose 6-13 complement C3 Homo sapiens 158-161 6379853-6 1984 Since agarose beads are activators of the alternative pathway of complement, and fibronectin is reported to bind to factor C3, we speculate that cell-derived C3b is bound to the beads and fibronectin-coated beads are ingested by the phagocytes via complement C3b receptors on the cells. Sepharose 6-13 fibronectin 1 Homo sapiens 188-199 6736244-5 1984 AFp, purified by affinity chromatography on retinol-binding protein linked to Sepharose, resembled plasma TTR in forming a stable tetrameric structure, and in its binding affinities for both thyroxine and retinol-binding protein. Sepharose 78-87 alpha fetoprotein Homo sapiens 0-3 6733150-8 1984 Antibody from one clone was coupled to CNBr-activated Sepharose 4B and the monoclonal antibody-Sepharose was shown to be very useful to isolate rat dopamine beta-monooxygenase from crude preparations. Sepharose 95-104 dopamine beta-hydroxylase Rattus norvegicus 148-175 6329306-6 1984 Here we show that kringle 4, carboxymethylated on Cys-1 and Cys-79, regains its lysine-Sepharose affinity following denaturation and reductive cleavage of its disulphide bonds. Sepharose 87-96 cystin 1 Homo sapiens 50-55 6734820-4 1984 In the column experiments stoichiometric amounts of calmodulin were retained on the spectrin-Sepharose column when micromolar concentrations of calcium were present. Sepharose 93-102 calmodulin 1 Homo sapiens 52-62 6331583-8 1984 After chromatography on Sepharose 6B-100 columns, 3 forms (a, c, d) of cholinesterase activity could be separated from homogenates of single ganglia. Sepharose 24-33 butyrylcholinesterase Rattus norvegicus 71-85 6146523-1 1984 Three cyclic AMP-independent acetyl-CoA carboxylase kinases (A, B1 and B2) have been isolated from lactating rat mammary gland, using phosphocellulose chromatography, high performance gel filtration, and affinity chromatography on casein-Sepharose and phosvitin-Sepharose. Sepharose 238-247 UDP glucuronosyltransferase family 1 member A1 Rattus norvegicus 60-74 6146523-1 1984 Three cyclic AMP-independent acetyl-CoA carboxylase kinases (A, B1 and B2) have been isolated from lactating rat mammary gland, using phosphocellulose chromatography, high performance gel filtration, and affinity chromatography on casein-Sepharose and phosvitin-Sepharose. Sepharose 262-271 UDP glucuronosyltransferase family 1 member A1 Rattus norvegicus 60-74 6433740-0 1984 Purification of pepsins and cathepsin D by affinity chromatography on Sepharose 4B with an immobilized synthetic inhibitor. Sepharose 70-82 cathepsin D Homo sapiens 28-39 6234943-1 1984 A proteinase which can activate human, dog and rat plasminogen to plasmin has been isolated from the urine of female rats, using affinity chromatography on benzamidine-coupled Sepharose. Sepharose 176-185 endogenous retrovirus group K member 25 Homo sapiens 2-12 6547836-9 1984 The Mr of this elastin mRNA, determined by methylmercury-agarose-gel electrophoresis, was approx. Sepharose 57-64 elastin Ovis aries 15-22 6233283-2 1984 Following chromatography on Sepharose 4B and hydroxylapatite the Ca2+-ATPase had a specific activity of 1.1 mumol of ATP hydrolyzed/min/mg of protein at 30 degrees C. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that 80% of the preparation was a polypeptide with a molecular weight of approximately 100,000. Sepharose 28-37 dynein axonemal heavy chain 8 Homo sapiens 70-76 6327694-8 1984 For example, one-half of the fibronectin-Sepharose-binding fraction from the long-term sites could also bind to platelet factor 4-Sepharose; however, over 90% of the fibronectin-binding fraction from newly formed sites could bind to platelet factor 4. Sepharose 41-50 fibronectin 1 Homo sapiens 29-40 6327694-8 1984 For example, one-half of the fibronectin-Sepharose-binding fraction from the long-term sites could also bind to platelet factor 4-Sepharose; however, over 90% of the fibronectin-binding fraction from newly formed sites could bind to platelet factor 4. Sepharose 130-139 fibronectin 1 Homo sapiens 29-40 6327694-9 1984 A major portion of the octyl-Sepharose-binding fractions of the original extracts could bind to fibronectin-Sepharose. Sepharose 29-38 fibronectin 1 Homo sapiens 96-107 6733116-3 1984 Calmodulin and S-100 protein were eluted from the aminoocytl -agarose column with 1 mM EGTA in the presence of 0.15 M NaCl and the elution of troponin-C was Ca2+-independently carried out with 0.3 M NaCl. Sepharose 62-69 calmodulin 1 Homo sapiens 0-10 6733116-3 1984 Calmodulin and S-100 protein were eluted from the aminoocytl -agarose column with 1 mM EGTA in the presence of 0.15 M NaCl and the elution of troponin-C was Ca2+-independently carried out with 0.3 M NaCl. Sepharose 62-69 S100 calcium binding protein B Homo sapiens 15-20 6733118-0 1984 Elution of interferon beta from blue sepharose by poly(vinylpyrrolidone). Sepharose 37-46 interferon beta 1 Homo sapiens 11-26 6740570-4 1984 HRH was fractionated further by chromatography on a column of AT-III bound to concanavalin A-Sepharose. Sepharose 93-102 serpin family C member 1 Homo sapiens 62-68 6375731-4 1984 Lipase activity and 14C-labeled protein co-eluted from heparin-Sepharose 4B at 1.2 M NaCl and were inhibited and precipitated by preincubation with anti-lipoprotein lipase gamma-globulins. Sepharose 63-75 lipase G, endothelial type Rattus norvegicus 0-6 6375731-4 1984 Lipase activity and 14C-labeled protein co-eluted from heparin-Sepharose 4B at 1.2 M NaCl and were inhibited and precipitated by preincubation with anti-lipoprotein lipase gamma-globulins. Sepharose 63-75 lipase G, endothelial type Rattus norvegicus 165-171 6433740-2 1984 Its bonding to activated 6-aminohexanoic acid-Sepharose 4B afforded an affinity support suitable for the purification of human, porcine, and chicken pepsin, human gastricsin, and bovine cathepsin D. Sepharose 46-55 progastricsin Homo sapiens 163-173 6433740-2 1984 Its bonding to activated 6-aminohexanoic acid-Sepharose 4B afforded an affinity support suitable for the purification of human, porcine, and chicken pepsin, human gastricsin, and bovine cathepsin D. Sepharose 46-55 cathepsin D Bos taurus 186-197 6426548-4 1984 Examination of the multimeric structure of the remaining unadsorbed FVIII/vWF protein by agarose gel electrophoresis and autoradiography showed that the largest multimers had been adsorbed to the collagen. Sepharose 89-96 coagulation factor VIII Homo sapiens 68-73 6426548-4 1984 Examination of the multimeric structure of the remaining unadsorbed FVIII/vWF protein by agarose gel electrophoresis and autoradiography showed that the largest multimers had been adsorbed to the collagen. Sepharose 89-96 von Willebrand factor Homo sapiens 74-77 6426550-10 1984 Furthermore, the inhibitor bound to agarose-protein A inactivated and removed PTA selectively from normal plasma. Sepharose 36-43 coagulation factor XI Homo sapiens 78-81 6430610-8 1984 The reaction of 125I-F-42 from the seven patients was positive with Sepharose bound antisera to IgG, kappa, lambda, gamma 1, gamma 2, gamma 3 and in one case to gamma 4. Sepharose 68-77 tryptophanyl-tRNA synthetase 1 Homo sapiens 116-141 6467084-4 1984 Intact clusterin binds quantitatively to wheat germ agglutinin - Sepharose 6 MB, but after treatment with neuraminidase only 49% specifically binds. Sepharose 65-74 clusterin Rattus norvegicus 7-16 6234177-3 1984 136: 111) using radiolabeling methods, we characterized from the membrane of the human B lymphoblastoid cell line Raji, a 140 000-Mr glycoprotein (gp140) carrying a C3b-binding activity with 125I-labeled C3b or Sepharose-bound C3b. Sepharose 211-220 endogenous retrovirus group K member 3 Homo sapiens 165-168 6327252-2 1984 CSF proteins in 107 children ranging from 3 to 24 months of age were analyzed by means of quantitative zone electrophoresis on agarose gel. Sepharose 127-134 colony stimulating factor 2 Homo sapiens 0-3 6427113-6 1984 Fractionation on Sepharose 4B indicated that 11S secretory IgA is the predominant form of colostral and milk antibody for all of the test bacteria, including B. abortus. Sepharose 17-26 CD79a molecule Homo sapiens 59-62 6431266-7 1984 Furthermore, absorption of mIgG on BSA-Sepharose did not lead to impairment of its CIICP-supporting effect. Sepharose 39-48 immunoglobulin heavy variable V1-62 Mus musculus 27-31 16663612-2 1984 Nitrate reductase (NR) in the crude extract was stable for several days at 0 degrees C and for several months at -80 degrees C. The enzyme was purified using (NH(4))(2)SO(4) fractionation, brushite-hydroxyl-apatite chromatography and blue-sepharose affinity chromatography. Sepharose 239-248 nitrate reductase [NADH] 1 Zea mays 0-17 6203118-4 1984 The purified receptor was eluted from insulin-Sepharose with 0.5 M NaCl and 1 mM dithiothreitol at pH 5.5. Sepharose 46-55 insulin Homo sapiens 38-45 6722173-1 1984 A homogeneous cytochrome P-450scc preparation with a specific enzyme content of 18 nmol/1 mg protein has been obtained using affinity chromatography on adrenodoxin-Sepharose under optimal conditions of the protein adsorption onto and desorption from the affinity sorbent. Sepharose 164-173 cholesterol side-chain cleavage enzyme, mitochondrial Bos taurus 14-33 6725265-5 1984 PC/CO/apoB complexes exhibit beta-migration on agarose gels and show one high molecular weight protein band on 3.0% sodium dodecyl sulfate-polyacrylamide gels. Sepharose 47-54 apolipoprotein B Homo sapiens 6-10 6430120-1 1984 Human milk lysozyme was purified by heparin-Sepharose affinity chromatography and Sepharose 4B gel-permeation chromatography. Sepharose 44-53 lysozyme Homo sapiens 11-19 6430120-1 1984 Human milk lysozyme was purified by heparin-Sepharose affinity chromatography and Sepharose 4B gel-permeation chromatography. Sepharose 82-94 lysozyme Homo sapiens 11-19 6609839-2 1984 IL2 activity is retained by columns of ganglioside covalently linked to poly(L-lysine)-agarose and is not eluted with ethylene glycol but is completely recovered by elution with 1% SDS. Sepharose 87-94 interleukin 2 Homo sapiens 0-3 6742833-0 1984 Charge-shift electrophoretic behavior of T-2 toxin in agarose gels. Sepharose 54-61 solute carrier family 25 member 5 Homo sapiens 41-44 6145455-3 1984 Purification of arylsulfatase A was performed with a three-step procedure consisting of centrifugation (85,000 X g), affinity chromatography with p-aminobenzamidine-Sepharose followed by chromatography on diethyaminoethyl (DEAE) Sephadex. Sepharose 165-174 arylsulfatase A Oryctolagus cuniculus 16-31 6430612-4 1984 PAF has been purified from Epstein-Barr (EB) virus genome carrying lymphoid lines by affinity chromatography using lysine-Sepharose columns. Sepharose 122-131 PCNA clamp associated factor Homo sapiens 0-3 6743257-2 1984 Spermidine synthase (EC 2.5.1.16) was purified to apparent homogeneity (about 11 000-fold) from bovine brain by affinity chromatography, with S-adenosyl-(5")-3-thiopropylamine linked to Sepharose as the adsorbent. Sepharose 186-195 spermidine synthase Bos taurus 0-19 6376344-4 1984 An immunoaffinity column prepared from antibody coupled to Sepharose has been used in the purification of renin from hog kidney. Sepharose 59-68 LOW QUALITY PROTEIN: renin Oryctolagus cuniculus 106-111 6735346-4 1984 Furthermore, the soluble lipoprotein lipase of fat-pads was fractionated by heparin-Sepharose affinity chromatography. Sepharose 84-93 lipoprotein lipase Rattus norvegicus 25-43 6735346-7 1984 The heterogeneity of lipoprotein lipase of rat adipose tissues was ensured using affinity chromatography on heparin-Sepharose. Sepharose 116-125 lipoprotein lipase Rattus norvegicus 21-39 6325422-1 1984 A new, rapid method for purification of calmodulin-stimulated phosphodiesterase from bovine, ovine, and porcine brain using only DEAE-agarose and calmodulin-Sepharose chromatography is described. Sepharose 157-166 calmodulin Bos taurus 40-50 6477350-1 1984 Conjugates of the monoclonal antibody anti-Thy 1:1 (OX7) and ricin have been constructed, using a thioether bond, such that the ricin no longer can bind Sepharose or asialofetuin. Sepharose 153-162 thymus cell antigen 1, theta Mus musculus 43-50 6477350-5 1984 The W3/25-ricin conjugate which had lost Sepharose-binding capacity was toxic to W3/25 antigen-expressing rat T-leukaemia cells. Sepharose 41-50 Cd4 molecule Rattus norvegicus 4-9 6746841-0 1984 Adsorption properties of different hepatitis B virus related antigens (HBsAg, HBcAg, HBeAg) on octanohydrazide-Sepharose 4B. Sepharose 111-120 capsid protein;pre-capsid protein Hepatitis B virus 85-90 24253644-2 1984 Cyanogen-bromide-activated agarose beads were coated with protein (gelatine or bovine serum albumin) and lectins were subsequently linked to the protein layer using glutaraldehyde. Sepharose 27-34 albumin Homo sapiens 86-99 6325418-4 1984 Both activities are bound to the same extent on insulin-Sepharose, and the immobilized kinase, after extensive washing, exhibits activity versus histone, which closely approaches that of the insulin-stimulated, solubilized kinase. Sepharose 56-65 insulin Homo sapiens 48-55 6325418-4 1984 Both activities are bound to the same extent on insulin-Sepharose, and the immobilized kinase, after extensive washing, exhibits activity versus histone, which closely approaches that of the insulin-stimulated, solubilized kinase. Sepharose 56-65 insulin Homo sapiens 191-198 6715316-5 1984 Newly synthesized cathepsin D was isolated by butanol/Triton X-100 extraction and immunoadsorption with anti-cathepsin D IgG-Sepharose, and the isomeric forms were separated by two-dimensional electrophoresis and fluorography. Sepharose 125-134 cathepsin D Oryctolagus cuniculus 18-29 6325021-1 1984 Calmodulin (Cam) was isolated from normal and from transformed human lymphocytes by affinity chromatography on CAPP-Sepharose 4B, followed by chromatofocusing. Sepharose 116-128 calmodulin 1 Homo sapiens 0-10 6325021-1 1984 Calmodulin (Cam) was isolated from normal and from transformed human lymphocytes by affinity chromatography on CAPP-Sepharose 4B, followed by chromatofocusing. Sepharose 116-128 calmodulin 1 Homo sapiens 12-15 6331390-6 1984 Cytochrome b-245 was chromatographed on a column of heparin-agarose and eluted with NaCl to give a peak specific content of 11-16 nmol of cytochrome b-245/mg of protein, representing a 140-200-fold purification with a recovery of 15%. Sepharose 60-67 mitochondrially encoded cytochrome b Homo sapiens 0-12 6608447-4 1984 Fluorescence-activated cell sorter (FACS) analyses revealed that the fraction of a monoclonal anti-Thy1.1-ricin conjugate that passed through a Sepharose column had markedly diminished capacity to bind non-specifically to Thy1.2-expressing CBA thymocytes and EL4 lymphoma cells. Sepharose 144-153 thymus cell antigen 1, theta Mus musculus 99-105 6608447-9 1984 By contrast, the fraction of anti-Thy1.1-ricin that retained Sepharose-binding capacity showed marked non-specific binding and toxicity to EL4 cells. Sepharose 61-70 thymus cell antigen 1, theta Mus musculus 34-40 6426471-2 1984 Aldehyde reductase (aldose reductase) was purified to homogeneity (as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis) from bovine lens by affinity chromatography on NADP+-Sepharose. Sepharose 193-202 aldo-keto reductase family 1 member B1 Bos taurus 0-18 6426471-2 1984 Aldehyde reductase (aldose reductase) was purified to homogeneity (as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis) from bovine lens by affinity chromatography on NADP+-Sepharose. Sepharose 193-202 aldose reductase Bos taurus 20-36 6546700-12 1984 However, thrombin Metz binds less tightly to a heparin-Sepharose column, and the direct inhibition of heparin on its activity on S2238 is weaker. Sepharose 55-64 coagulation factor II, thrombin Homo sapiens 9-17 6145647-0 1984 Application of a Phaseolus vulgaris erythroagglutinating lectin agarose column for the specific detection of human hepatoma gamma-glutamyl transpeptidase in serum. Sepharose 64-71 inactive glutathione hydrolase 2 Homo sapiens 124-153 6201493-8 1984 When a saponin-permeabilized, agarose-embedded plasma membrane (PM) fraction was incubated with affinity-purified anti-LAP, 85% of the protein A-gold particles associated with the three recognizable PM domains were present in the BC. Sepharose 30-37 leucine aminopeptidase 3 Rattus norvegicus 119-122 6233389-2 1984 This method reduces anodal gel shrinkage and shortens development time when compared to the conventional electrophoretic technique for PGM subtyping which is performed at pH 7.4 using an agarose-starch substrate. Sepharose 187-194 phosphoglucomutase 1 Homo sapiens 135-138 6707579-3 1984 Although the binding to agarose, a linear galactan hydrocolloid derived from some marine algae, is unlikely per se to be related to the physiological function of SAP, it does provide a model system in which to explore the precise ligand requirements of SAP. Sepharose 24-31 amyloid P component, serum Homo sapiens 253-256 6707579-4 1984 We report here that the amount of SAP from human, mouse, and plaice (Pleuronectes platessa L.) serum able to bind to agarose from different sources reflect precisely their pyruvate content. Sepharose 117-124 amyloid P component, serum Homo sapiens 34-37 6707579-5 1984 Methylation with diazomethane of the carboxyl groups in the pyruvate moiety of agarose completely abolishes SAP binding to agarose. Sepharose 79-86 amyloid P component, serum Homo sapiens 108-111 6707579-5 1984 Methylation with diazomethane of the carboxyl groups in the pyruvate moiety of agarose completely abolishes SAP binding to agarose. Sepharose 123-130 amyloid P component, serum Homo sapiens 108-111 6427377-2 1984 Plasma apoE and the primary translation product migrated similarly on SDS-polyacrylamide gels, had similar partial proteolytic peptide maps, and bound to and coeluted from heparin-Sepharose columns. Sepharose 180-189 apolipoprotein E Rattus norvegicus 7-11 6584526-4 1984 Agarose, another insoluble glycan containing an alternating sequence of the disaccharide beta-1,3-D-galactose-alpha-1,4-3,6-anhydro-L-galactose units was less potent. Sepharose 0-7 calcium channel, voltage-dependent, beta 3 subunit Mus musculus 89-97 6328538-1 1984 Ultrogel acrylamide-agarose chromatography was employed for fractionation of oxytocinase isoenzymes from serum of pregnant women and from human placenta. Sepharose 20-27 leucyl and cystinyl aminopeptidase Homo sapiens 77-88 6330931-1 1984 Mouse C1q, a subcomponent of the first component of complement, was purified from mouse EDTA plasma by a combination of precipitation with polyethyleneglycol, affinity chromatography on IgG-Sepharose, ion exchange chromatography and molecular sieving. Sepharose 190-199 complement component 1, q subcomponent, alpha polypeptide Mus musculus 6-9 6715452-2 1984 The relative affinities of Sepharose gels, to which coenzyme A (CoA-SH) and CoA-SH analogues were bound through a well defined site, for citrate synthase were determined. Sepharose 27-36 citrate synthase Sus scrofa 137-153 6321505-7 1984 The mRNA encoded by the parathyroid hormone gene consists of 800 +/- 50 nucleotides as determined by electrophoresis on agarose gels. Sepharose 120-127 parathyroid hormone Rattus norvegicus 24-43 6365918-2 1984 Two types of trypsin-like proteases, spermosin and acrosin, have been highly purified from spermatozoa of the ascidian (Prochordata) Halocynthia roretzi by a procedure including diethylaminoethylcellulose chromatography, Sephadex G-100 gel filtration, and soybean trypsin inhibitor-immobilized Sepharose 4B chromatography. Sepharose 294-306 acrosin Homo sapiens 51-58 6699012-1 1984 The unactivated, molybdate-stabilized rat hepatic glucocorticoid receptor has been purified approximately 4000-fold, as calculated by specific radioactivity, by affinity chromatography using a deoxycorticosterone-derivatized agarose, gel filtration on Bio-Gel A-1.5m agarose, and DEAE-cellulose chromatography. Sepharose 225-232 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 50-73 6731828-0 1984 Purification of S-acyl fatty acid synthase thioester hydrolase by affinity chromatography with fatty acid synthase attached to Sepharose. Sepharose 127-136 fatty acid synthase Homo sapiens 23-42 6731828-0 1984 Purification of S-acyl fatty acid synthase thioester hydrolase by affinity chromatography with fatty acid synthase attached to Sepharose. Sepharose 127-136 fatty acid synthase Homo sapiens 95-114 6731828-1 1984 S-Acyl fatty acid synthase thioester hydrolase, the enzyme responsible for release of short-chain fatty acids from fatty acid synthase, was purified to homogeneity in 50-60% yield by a procedure involving affinity chromatography with fatty acid synthase as the affinity ligand attached to Sepharose. Sepharose 289-298 fatty acid synthase Homo sapiens 7-26 6367662-1 1984 Insulin protease was purified 700-fold from rat liver homogenate by combined ultracentrifugation, ammonium sulfate fractionation, and glucagon-Sepharose-4B affinity chromatography. Sepharose 143-152 insulin degrading enzyme Rattus norvegicus 0-16 6367662-6 1984 When supernatants, recovered from rat liver homogenates after centrifugation at 100,000g, were subjected to combined Sepharose 4B-insulin protease affinity chromatography and dialysis, a potent inhibitor of insulin protease was obtained which was heat stable. Sepharose 117-126 insulin degrading enzyme Rattus norvegicus 207-223 6607753-3 1984 DIF was purified 7,000-fold from HUT-102 conditioned media by utilizing ion-exchange chromatography with DEAE-Sepharose, gel chromatography, Blue-Sepharose chromatography, and preparative SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Sepharose 110-119 tumor necrosis factor Homo sapiens 0-3 6607757-10 1984 By SDS-agarose electrophoresis and radioimmunoblotting, the vWF within the cytosol of the endothelial cells was found to possess a multimeric pattern similar to that found for either purified plasma vWF or vWF released into media overlying endothelial cell cultures. Sepharose 7-14 von Willebrand factor Homo sapiens 60-63 6609779-3 1984 The analysis of total poly A + containing RNA from PHA stimulated T lymphocytes on agarose gels reveals the existence of at least two different IL-2 mRNAs. Sepharose 83-90 interleukin 2 Homo sapiens 144-148 6373594-8 1984 Latent renin showed affinity for pepstatin-Sepharose gel. Sepharose 43-52 renin Rattus norvegicus 7-12 6198333-3 1984 Further characterization showed that ovarian follicular PAPP-A bound reversibly to heparin-Sepharose. Sepharose 91-100 pappalysin 1 Homo sapiens 56-62 6230367-10 1984 Hepatoma heparan sulfate proteoglycans purified from the culture medium had a considerably lower affinity for fibronectin-Sepharose compared with that of rat liver heparan sulfate proteoglycans. Sepharose 122-131 fibronectin 1 Rattus norvegicus 110-121 6699545-4 1984 The activation of zymosan was C3 dependent, and C3b-coated Sepharose was also an effective stimulant. Sepharose 59-68 complement C3 Homo sapiens 48-51 6231642-2 1984 Soluble or Sepharose-bound anti-Ti monoclonal antibodies, like physiologic ligand, enhanced proliferative responses to purified IL-2 by inducing a 6-fold increase in surface IL-2 receptor expression. Sepharose 11-20 interleukin 2 Homo sapiens 128-132 6231642-2 1984 Soluble or Sepharose-bound anti-Ti monoclonal antibodies, like physiologic ligand, enhanced proliferative responses to purified IL-2 by inducing a 6-fold increase in surface IL-2 receptor expression. Sepharose 11-20 interleukin 2 receptor subunit beta Homo sapiens 174-187 6231642-3 1984 In contrast, only Sepharose-bound anti-Ti or physiologic ligand triggered endogenous clonal IL-2 production and resulted in subsequent proliferation. Sepharose 18-27 interleukin 2 Homo sapiens 92-96 6719385-1 1984 Interaction of human antithrombin III (AT III) with human alpha-thrombin coupled to Sepharose 4B was investigated. Sepharose 84-93 serpin family C member 1 Homo sapiens 21-37 6719385-1 1984 Interaction of human antithrombin III (AT III) with human alpha-thrombin coupled to Sepharose 4B was investigated. Sepharose 84-93 serpin family C member 1 Homo sapiens 39-45 6719385-1 1984 Interaction of human antithrombin III (AT III) with human alpha-thrombin coupled to Sepharose 4B was investigated. Sepharose 84-93 coagulation factor II, thrombin Homo sapiens 25-33 6719385-7 1984 However, Sepharose-coupled thrombin mixed with plasma in the presence of heparin produced outstanding quantities of residual immunoreactive AT III devoid of inhibitory activity. Sepharose 9-18 coagulation factor II, thrombin Homo sapiens 27-35 6719385-7 1984 However, Sepharose-coupled thrombin mixed with plasma in the presence of heparin produced outstanding quantities of residual immunoreactive AT III devoid of inhibitory activity. Sepharose 9-18 serpin family C member 1 Homo sapiens 140-146 6696911-3 1984 3 S. The specificity of the interaction of this androgen receptor with nucleotides was investigated in a competitive binding assay using inhibition of binding of the steroid receptor complex to ADP-Sepharose. Sepharose 198-207 androgen receptor Rattus norvegicus 48-65 6724054-1 1984 The purification of human uterine estrogen receptor (ER) was performed by way of the affinity chromatography technique using immobilized 17 beta-estradiol on Sepharose 4B, which was developed by Sica and Bresciani (Biochemistry, 18: 2369-2378, 1979). Sepharose 158-170 estrogen receptor 1 Homo sapiens 34-51 6724054-1 1984 The purification of human uterine estrogen receptor (ER) was performed by way of the affinity chromatography technique using immobilized 17 beta-estradiol on Sepharose 4B, which was developed by Sica and Bresciani (Biochemistry, 18: 2369-2378, 1979). Sepharose 158-170 estrogen receptor 1 Homo sapiens 53-55 6200074-1 1984 A method is reported for the isolation of histone TH2B-x from rat testis by affinity chromatography on an agarose-p-chloromercurianilino column. Sepharose 106-113 H2B clustered histone 1 Rattus norvegicus 50-54 6089822-1 1984 The binding of calmodulin to spectrin from human erythrocytes has been studied by affinity chromatography on sepharose-calmodulin column. Sepharose 109-118 calmodulin 1 Homo sapiens 15-25 6089822-2 1984 The alpha and beta spectrin chains, dissociated in 6-7 M urea, both bound to the sepharose-calmodulin column, but with different affinities. Sepharose 81-90 calmodulin 1 Homo sapiens 91-101 6370230-3 1984 Because it is difficult to differentiate the atypical ADH2 2-2 phenotype from the ADH2 2-1 phenotype by starch gel electrophoresis, an agarose isoelectric focusing procedure was developed that clearly separated the atypical Japanese livers into two groups, A1 and A2. Sepharose 135-142 alcohol dehydrogenase 1B (class I), beta polypeptide Homo sapiens 54-58 6696440-4 1984 Affinity chromatography on lentil lectin-agarose demonstrated that 84% of the dopamine beta-hydroxylase biantennary oligosaccharides are substituted by fucose on the core N-acetylglucosamine which is linked to asparagine. Sepharose 41-48 dopamine beta-hydroxylase Bos taurus 78-103 6712599-6 1984 Translated calmodulin was identified by its heat-stability, its co-migration with authentic anterior-pituitary calmodulin on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, its acidic isoelectric point (4.15) on flat-bed isoelectric focusing, its Ca2+-dependent binding to fluphenazine-Sepharose 6B, and its co-elution from this gel with authentic unlabelled calmodulin with EGTA buffer. Sepharose 298-307 calmodulin-3 Sus scrofa 11-21 6712769-4 1984 The new apolipoprotein component, named apo E-5, was identical with ordinary apo E in apparent molecular weight by SDS-polyacrylamide gel electrophoresis and in its interactions with heparin-Sepharose gel and with anti-apo E antibody. Sepharose 191-200 apolipoprotein E Homo sapiens 40-45 6203557-9 1984 One of the components from dissociated LDLe containing the immunodeterminant B-III, has been separated by chromatography on heparin-agarose. Sepharose 132-139 calcium voltage-gated channel subunit alpha1 B Homo sapiens 77-82 6607106-7 1984 After serum perfusion over PACC, protein A was identified in the effluent by additional studies as follows: (a) polyacrylamide gel electrophoresis analysis showed that eluted 125I-protein A comigrated with the protein A marker; (b) postperfusion C1q-binding complexes, isolated by gel filtration under dissociating conditions and affinity chromatography on IgG-Sepharose showed a single precipitin band with normal human (protein A reactive) but not chicken (protein A unreactive) serum. Sepharose 361-370 G protein-coupled receptor 162 Gallus gallus 33-42 6141286-5 1984 The inhibitory effect of MY-5445 on cyclic GMP phosphodiesterase was also demonstrated by direct binding of the enzyme to MY-5445 coupled Sepharose, which was a useful tool for purifying the cyclic GMP phosphodiesterase from human platelet. Sepharose 138-147 5'-nucleotidase, cytosolic II Homo sapiens 43-46 6141286-5 1984 The inhibitory effect of MY-5445 on cyclic GMP phosphodiesterase was also demonstrated by direct binding of the enzyme to MY-5445 coupled Sepharose, which was a useful tool for purifying the cyclic GMP phosphodiesterase from human platelet. Sepharose 138-147 5'-nucleotidase, cytosolic II Homo sapiens 198-201 6198401-6 1984 Keratinocytes produced soluble fibronectin, since both immunoprecipitation and adsorption to gelatin-Sepharose detected 35S-methionine-labeled material which comigrated with human plasma fibronectin on sodium dodecyl sulfate polyacrylamide gels. Sepharose 101-110 fibronectin 1 Homo sapiens 31-42 6694247-6 1984 Labelled 75Se fibronectin was purified from donor rat plasma by gelatin-sepharose affinity chromatography. Sepharose 72-81 fibronectin 1 Homo sapiens 14-25 6366788-2 1984 Purification of OrnDCase mRNA from kidneys of androgen-treated mice was accomplished by immunoadsorption of renal polysomes to a protein A-Sepharose column and enrichment for poly(A)-containing RNA by oligo(dT)-cellulose. Sepharose 139-148 ornithine decarboxylase, structural 1 Mus musculus 16-24 6367010-7 1984 The results obtained with the two methods applied on a freshly prepared and a stored fibronectin are in agreement with sodium dodecylsulphate polyacrylamide gel electrophoresis followed by immunoblotting before and after gelatin-Sepharose adsorption. Sepharose 229-238 fibronectin 1 Homo sapiens 85-96 6367010-8 1984 These techniques demonstrate that all the freshly prepared fibronectin adsorbs to gelatin-Sepharose, while stored fibronectin, which is broken down to numerous peptides, still reacts with the fibronectin antibody, but does not adsorb to gelatin-Sepharose. Sepharose 90-99 fibronectin 1 Homo sapiens 59-70 6229533-4 1984 Extraction of HMG-17 from chromatin by treatment with 0.4 M NaCl abolished the specific binding of acetylated chromatin to the Sepharose columns. Sepharose 127-136 high mobility group nucleosomal binding domain 2 Homo sapiens 14-20 6320121-1 1984 The duplex DNA unwinding ability of Xenopus transcription factor A was examined by treatment of plasmid-protein complexes with calf thymus topoisomerase I and resolution of the topoisomer distributions by agarose gel electrophoresis. Sepharose 205-212 general transcription factor 3A L homeolog Xenopus laevis 58-66 6421285-5 1984 The affinity adsorbent alpha-lactalbumin-agarose, which did not retard GM2 ganglioside galactosyltransferase, was used to remove most or all of galactosyltransferase activity towards glycoprotein and monosaccharide acceptors from the extracted Golgi preparation. Sepharose 41-48 lactalbumin, alpha Rattus norvegicus 23-40 6421285-6 1984 After treatment of the extracted Golgi preparation with alpha-lactalbumin-agarose, enzyme I and enzyme II GM2 ganglioside galactosyltransferase activities, prepared by using DEAE-Sepharose chromatography, were distinguishable from transferase activity towards GM2 oligosaccharide and glycoproteins by the criterion of thermolability. Sepharose 74-81 lactalbumin, alpha Rattus norvegicus 56-73 6696878-5 1984 Cathepsin G substrate and renin substrate activities copurified during Affi-Gel Blue affinity chromatography, hydroxylapatite chromatography, phenyl-Sepharose chromatography, and S-200 gel filtration. Sepharose 149-158 cathepsin G Homo sapiens 0-11 6696878-5 1984 Cathepsin G substrate and renin substrate activities copurified during Affi-Gel Blue affinity chromatography, hydroxylapatite chromatography, phenyl-Sepharose chromatography, and S-200 gel filtration. Sepharose 149-158 renin Homo sapiens 26-31 6231286-8 1984 The Mr = 90,000 fragment can be isolated on calmodulin-Sepharose and on phenothiazine affinity columns. Sepharose 55-64 calmodulin 1 Homo sapiens 44-54 6242454-7 1984 Addition of agarose to in vitro and in vivo systems selectively suppressed the elastin production but did not diminish the production of elastic fibre microfibrils and other matrix components. Sepharose 12-19 elastin Oryctolagus cuniculus 79-86 6380173-1 1984 Messenger RNAs for the alpha 1 and alpha 2 chains of type I procollagen were partially purified from total embryonic chicken calvaria using gel chromatography on Sepharose 4B and used to construct recombinant cDNA clones corresponding to both mRNAs. Sepharose 162-171 asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) Gallus gallus 23-42 6208756-6 1984 Migration under agarose was decreased with all of the IFN but phagocytosis and release of enzymes was not affected. Sepharose 16-23 interferon alpha 1 Homo sapiens 54-57 6611291-5 1984 Two of them, PI N GRO and PI N YER, could not be separated by isoelectric focusing, but gave a different pattern in agarose gel electrophoresis. Sepharose 116-123 dynein light chain LC8-type 1 Homo sapiens 13-17 6696761-1 1984 Human erythrocyte glucose-6-phosphate dehydrogenase was purified to homogeneity by a simplified procedure, consisting of 2",5"-ADP-Sepharose affinity chromatography, followed by Sephadex G-100 gel filtration. Sepharose 131-140 glucose-6-phosphate dehydrogenase Homo sapiens 18-51 6370033-1 1984 Elongation factor Tu from Escherichia coli or Thermus thermophilus was immobilized on cyanogen bromide-activated Sepharose. Sepharose 113-122 eukaryotic translation elongation factor 1 alpha 1 Bos taurus 0-20 6524707-1 1984 Two-dimensional agarose gel (pH 8.6)-horizontal polyacrylamide gel (pH 9.0) electrophoresis of horse serum proteins revealed genetic polymorphism of ceruloplasmin (Cp) and two unidentified serum proteins tentatively designated serum protein 1 (SP1) and serum protein 2 (SP2). Sepharose 16-23 ceruloplasmin Equus caballus 149-162 6329161-1 1984 Preparations of horse heart cytochrome c have been obtained immobilized on Sepharose derivatives via lysine epsilon-amino groups, carboxyl groups of aspartic and glutamic acid residues, methionine and histidine residues as well as imidazole groups additionally introduced by means of modification of free carboxyl groups by histamine. Sepharose 75-84 cytochrome c, somatic Equus caballus 28-40 6329161-2 1984 Dissociation constants have been determined for complexes of adrenodoxin, hepatoredoxin , cytochrome b5 heme-containing tryptic fragment and myoglobin with cytochrome c preparations immobilized via lysine residues (cytochrome c-Sepharose I) or additional imidazole groups (cytochrome c-Sepharose II). Sepharose 228-237 cytochrome b Equus caballus 90-102 6329161-2 1984 Dissociation constants have been determined for complexes of adrenodoxin, hepatoredoxin , cytochrome b5 heme-containing tryptic fragment and myoglobin with cytochrome c preparations immobilized via lysine residues (cytochrome c-Sepharose I) or additional imidazole groups (cytochrome c-Sepharose II). Sepharose 286-295 cytochrome b Equus caballus 90-102 6329161-5 1984 The efficiency of cytochrome c-Sepharose II application in the course of adrenodoxin, hepatoredoxin and cytochrome b5 purification, as well as isolation of cytochrome b4 heme-containing tryptic fragment has been shown. Sepharose 31-40 cytochrome c, somatic Equus caballus 18-30 6205820-3 1984 Hare uteroglobin mRNA was about 40 nucleotides larger than the homologous rabbit one, as analyzed by electrophoresis on agarose gel. Sepharose 120-127 uteroglobin Oryctolagus cuniculus 5-16 6434227-1 1984 Lactoferrin from human milk, pancreatic juice and bovine milk were purified by heparin-Sepharose affinity chromatography procedure. Sepharose 87-96 lactotransferrin Bos taurus 0-11 6509863-7 1984 Both ABG and the dimer can be separated from corticosteroid-binding globulin by Blue Sepharose chromatography. Sepharose 85-94 serpin family A member 6 Homo sapiens 45-76 6544185-2 1984 This partially denatured C3 (C3i) was coupled to activated Thiol-Sepharose via its single SH group. Sepharose 65-74 complement C3 Homo sapiens 25-27 6544185-2 1984 This partially denatured C3 (C3i) was coupled to activated Thiol-Sepharose via its single SH group. Sepharose 65-74 complement C3 Homo sapiens 29-32 6208030-7 1984 The results obtained demonstrate that carrier serum proteins investigated by agarose gel electrophoresis were in the migration zone of alpha 2-, alpha 1- and beta 1-globulins, whereas the radioactivity found in the serum albumin zone was negligible. Sepharose 77-84 adrenoceptor alpha 1D Homo sapiens 135-164 6394369-5 1984 Immobilization of this antibody on Sepharose 4B yields an efficient immunosorbent for purification of beta 2m. Sepharose 35-47 beta-2-microglobulin Homo sapiens 102-109 6437941-3 1984 The major contaminants, fibronectin and IgM, were removed by affinity chromatography on gelatin- and anti-IgM-agarose, respectively. Sepharose 110-117 fibronectin 1 Homo sapiens 24-35 6092254-7 1984 A solid-phase immunoadsorbent RIA specific for the extreme COOH-terminus of ACTH(1-24) was developed by coupling antiserum (A-6) to Sepharose 4B. Sepharose 132-141 proopiomelanocortin Homo sapiens 76-80 6498233-4 1984 The results presented here suggest that the two forms eluted from heparin-agarose correspond to the untransformed and transformed states of the glucocorticoid receptor complex. Sepharose 74-81 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 144-167 6539138-3 1984 Its apparent molecular weight, determined by the SDS-PAGE fibrin-agarose underlay method, was approximately 68,000 daltons, and its activity was quenched by antibodies against human tissue plasminogen activator (t-PA). Sepharose 65-72 plasminogen activator, tissue type Homo sapiens 182-216 6696429-1 1984 The mechanism of activation of thioredoxin-linked NADP-malate dehydrogenase was investigated by using 14C-iodoacetate and 14C-dansylated thioredoxin m, and Sepharose affinity columns (thioredoxin m, NADP-malate dehydrogenase) as probes to monitor enzyme sulfhydryl status and enzyme-thioredoxin interaction. Sepharose 156-165 LOC101027257 Zea mays 31-42 6421279-4 1984 The antibody attached to Sepharose could be used to form an immunoaffinity column that retained mammalian ornithine decarboxylase. Sepharose 25-34 ornithine decarboxylase 1 Homo sapiens 106-129 6569839-5 1984 Sepharose 2B chromatography indicated that secreted mucin was smaller in size than intracellular mucin and had compositional differences suggestive of "immaturity" or protein contamination. Sepharose 0-9 solute carrier family 13 member 2 Rattus norvegicus 52-57 6735273-4 1984 When washed platelets, resuspended in a buffer containing fibronectin, were filtered on a low-affinity collagen-Sepharose, a significant increase in their adhesion occurred. Sepharose 112-121 fibronectin 1 Homo sapiens 58-69 6735273-5 1984 A similar modification could be observed when platelets were allowed to adhere to the same collagen-Sepharose preconditioned with fibronectin. Sepharose 100-109 fibronectin 1 Homo sapiens 130-141 6430782-1 1984 The plasmas of two groups of subjects were examined for blood coagulation Factor XIII-A (FXIII-A, F13A) by electrophoresis in agarose using a Tris-EDTA-borate buffer to separate the common variants, F13A*1, F13A*2, and F13A*3. Sepharose 126-133 coagulation factor XIII A chain Homo sapiens 98-102 6384062-1 1984 Fc gamma receptor (FcR) from human placenta was solubilized using EDTA and 2-mercaptoethanol and purified by affinity chromatography on human IgG-coated Sepharose 4B. Sepharose 153-162 Fc gamma receptor Ia Homo sapiens 0-17 6384062-1 1984 Fc gamma receptor (FcR) from human placenta was solubilized using EDTA and 2-mercaptoethanol and purified by affinity chromatography on human IgG-coated Sepharose 4B. Sepharose 153-162 Fc gamma receptor Ia Homo sapiens 19-22 6611291-5 1984 Two of them, PI N GRO and PI N YER, could not be separated by isoelectric focusing, but gave a different pattern in agarose gel electrophoresis. Sepharose 116-123 dynein light chain LC8-type 1 Homo sapiens 26-30 6434908-6 1984 In affinity chromatography of the LKF on a Con A-Sepharose column, MAF and IFN activities were found in both the nonadsorbing (F I) and adsorbing (F II) fractions. Sepharose 49-58 avian musculoaponeurotic fibrosarcoma oncogene homolog Mus musculus 67-70 6088322-4 1984 Galactosyltransferase immobilized onto agarose through its sulfhydryl group retained its ability to catalyze the synthesis of N-acetyllactosamine and lactose. Sepharose 39-46 N-acetyllactosaminide alpha-1,3-galactosyltransferase Bos taurus 0-21 6374044-1 1984 Thymosin fraction 5 restored the capacity of peripheral blood lymphocytes (PBL) from thymectomized adult guinea pigs sensitized with purified protein derivative (PPD) to produce migration inhibition factor (MIF) as measured in an agarose droplet microassay with peritoneal exudate cells. Sepharose 230-237 macrophage migration inhibitory factor Cavia porcellus 207-210 6208454-1 1984 Two structural variants of the rat alpha-fetoprotein (AFP) gene have been detected in different inbred strains of rats by EcoRI or HindIII restriction enzyme cleavage of cellular DNA, agarose gel electrophoresis and Southern blot hybridization using 32P-labeled cloned rat AFP cDNA probes. Sepharose 184-191 alpha-fetoprotein Rattus norvegicus 35-52 6208454-1 1984 Two structural variants of the rat alpha-fetoprotein (AFP) gene have been detected in different inbred strains of rats by EcoRI or HindIII restriction enzyme cleavage of cellular DNA, agarose gel electrophoresis and Southern blot hybridization using 32P-labeled cloned rat AFP cDNA probes. Sepharose 184-191 alpha-fetoprotein Rattus norvegicus 54-57 6323953-2 1984 In particular, several triazinyl dyes--Cibacron blue F3GA, Procion red HE3B, and Green A dye, immobilized to agarose via an ether linkage--retain specifically bound 1,25-dihydroxyvitamin D3-receptor complexes formed at 0-4 degrees which are eluted at high salt concentrations. Sepharose 109-116 vitamin D (1,25- dihydroxyvitamin D3) receptor Gallus gallus 165-198 6701471-4 1984 The binding and uptake of rabbit erythrocytes and agarose beads were proportional to the amount of C3b or C3bi on the particles. Sepharose 50-57 integrin alpha-M Ovis aries 106-110 6368594-5 1984 Ca 1 antibody was observed to bind to epithelial membrane antigen-coated sepharose beads. Sepharose 73-82 carbonic anhydrase 1 Homo sapiens 0-4 6516292-1 1984 A single-step method is described for the isolation of a highly purified antithrombin III (AT III) concentrate at a recovery of over 30% using affinity chromatography on heparin-Sepharose (HS). Sepharose 178-187 serpin family C member 1 Homo sapiens 73-89 6516292-1 1984 A single-step method is described for the isolation of a highly purified antithrombin III (AT III) concentrate at a recovery of over 30% using affinity chromatography on heparin-Sepharose (HS). Sepharose 178-187 serpin family C member 1 Homo sapiens 91-97 6528747-3 1984 Determinations were made after treatment of serum samples with neuraminidase by immunofixation on agarose gels. Sepharose 98-105 neuraminidase 1 Homo sapiens 63-76 6516292-3 1984 Pasteurization of the AT III concentrate in the presence of 0.5 M sodium citrate to minimize the risk of hepatitis decreases the recovery by about 20% and induces changes in the patterns obtained by polyacrylamide gel electrophoresis and by crossed immunoelectrophoresis in heparinized agarose gel. Sepharose 286-293 serpin family C member 1 Homo sapiens 22-28 6661431-1 1983 The androgen receptor has been purified from rat ventral prostate cytosol by a combination of differential DNA-Sepharose 4B chromatography and testosterone 17 beta-hemisuccinyl-3,3"-diaminodipropylamine-Sepharose 4B affinity chromatography. Sepharose 111-123 androgen receptor Rattus norvegicus 4-21 6361142-2 1983 We have adapted the agarose droplet MIF assay initially developed for guinea pig peritoneal exudate cells to utilize human monocytes. Sepharose 20-27 macrophage migration inhibitory factor Cavia porcellus 36-39 6661431-1 1983 The androgen receptor has been purified from rat ventral prostate cytosol by a combination of differential DNA-Sepharose 4B chromatography and testosterone 17 beta-hemisuccinyl-3,3"-diaminodipropylamine-Sepharose 4B affinity chromatography. Sepharose 203-215 androgen receptor Rattus norvegicus 4-21 6643486-4 1983 Fractionation of the thermolysin digest of fibronectin on the glycosaminoglycan-Sepharoses at low ionic strength revealed that three groups of fragments, i.e. Mr = 150,000-140,000, 24,000, and 16,000 (150K-140K, 24K, and 16K) fragments, were capable of binding to glycosaminoglycans with different specificities and affinities. Sepharose 80-90 fibronectin 1 Homo sapiens 43-54 6643486-10 1983 At physiologic ionic strength, only heparin-Sepharose could bind intact fibronectin. Sepharose 44-53 fibronectin 1 Homo sapiens 72-83 6643488-11 1983 Chromatography of phospholipase A1 on concanavalin A-Sepharose resulted in a loss of activity toward acidic phospholipids which could be restored with Ca2+. Sepharose 53-62 lipase H Homo sapiens 18-34 6364982-5 1983 By contrast, a 42-kDa chymotrypsin-generated Fn fragment which retains the ability to adhere to gelatin-Sepharose exhibited normal (noncooperative) binding to both gelatin fractions with Kd = 7 X 10(-7) M. In all cases, the increase in P could be reversed by addition of excess unlabeled gelatin or urea. Sepharose 104-113 fibronectin 1 Homo sapiens 45-47 6318728-2 1983 These isoenzymes were separated from one another by ion-exchange chromatography on DEAE-cellulose and by calmodulin-Sepharose affinity chromatography. Sepharose 116-125 calmodulin 2 Gallus gallus 105-115 6640109-7 1983 The abnormal antithrombin ("Chicago") was found to elute from heparin-Sepharose at a higher ionic strength than normal inhibitor. Sepharose 70-79 serpin family C member 1 Homo sapiens 13-25 6652976-9 1983 The opsonin was absorbed by immobilized Staphylococcus aureus and by gelatin-Sepharose, suggesting that it is related to fibronectin. Sepharose 77-86 fibronectin 1 Homo sapiens 121-132 6689271-1 1983 beta-Glucuronidase mRNA was purified from androgen-induced mouse kidney by immunoadsorption of polysomes to protein A-Sepharose. Sepharose 118-127 glucuronidase, beta Mus musculus 0-18 6652976-10 1983 Fibronectin from normal human sera prepared by affinity chromatography on gelatin-Sepharose was not opsonic in this system. Sepharose 82-91 fibronectin 1 Homo sapiens 0-11 6197305-1 1983 By affinity chromatography on concanavalin A (ConA) linked to Sepharose, S-carboxymethylated rat alpha-fetoprotein could be separated into two molecular variants: a ConA-reactive and a ConA-nonreactive fraction. Sepharose 62-71 alpha-fetoprotein Rattus norvegicus 97-114 6676104-2 1983 A highly purified transcortin was prepared from pooled human serum by the following four successive steps; ammonium sulfate fractionation, cortisol-Sepharose column chromatography, Ultragel AcAA column chromatography and hydroxylapatite column chromatography. Sepharose 148-157 serpin family A member 6 Homo sapiens 18-29 6432769-0 1983 Proteinase affinity chromatography on bacitracin-Sepharose. Sepharose 49-58 endogenous retrovirus group K member 25 Homo sapiens 0-10 6671971-1 1983 Prolidase [iminodipeptidase, EC 3.4.13.9] was highly purified from the cytosol fraction of bovine small intestine by a series of column chromatographies on DEAE-Toyopearl, Sephadex G-150, PCMB-T-Sepharose and hydroxyapatite. Sepharose 195-204 peptidase D Bos taurus 0-9 6196377-3 1983 In human plasma, 125I-Factor Xa bound to alpha 1-proteinase inhibitor, antithrombin III, and alpha 2-macroglobulin in a ratio of 4.9:1.9:1 as determined by gel electrophoresis and by adsorption to IgG-(antiproteinase inhibitor)-Sepharose beads. Sepharose 228-237 coagulation factor X Homo sapiens 22-31 6360207-3 1983 The renin component was purified further by passage through an anti-rat spleen cathepsin D immunoglobulin G-Sepharose (IgG-Sepharose) column followed by carboxymethyl-Sephadex (CM-Sepharose) chromatography which separated two renin components. Sepharose 108-117 renin Rattus norvegicus 4-9 6317500-2 1983 Glucokinase from rat liver or transplantable, radiation-induced insulinomas was partially purified by ion exchange chromatography using DEAE-Cibacron Blue F3GA agarose. Sepharose 160-167 glucokinase Rattus norvegicus 0-11 6139373-10 1983 A tryptic peptide containing the 3H label was isolated following digestion of [3H]C3b on activated thiol-Sepharose. Sepharose 105-114 complement C3 Homo sapiens 82-85 6440856-1 1984 The protein A-binding site of human IgM was studied by affinity chromatography on SpA-Sepharose using fragments derived from a human monoclonal SpA-reactive IgM, Iz. Sepharose 86-95 surfactant protein A1 Homo sapiens 82-85 6500632-2 1984 Macrophages migrate under agarose to medium conditioned by the EL-4 cell line for up to 40 h. EL-4 conditioned medium after 67 h contained dialyzable migration inhibitors. Sepharose 26-33 epilepsy 4 Mus musculus 94-98 6651850-1 1983 Several rat liver cytochromes P-450 have been substantially purified in a one-step immunoadsorption procedure using Sepharose-bound monoclonal antibodies (MAbs) to the major forms of rat liver cytochrome P-450 induced by 3-methylcholanthrene and phenobarbital (MC-P-450 and PB-P-450, respectively). Sepharose 116-125 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 193-209 6313678-1 1983 Cytochrome c has been covalently attached to Sepharose 6MB to study the effects of immobilization on the molecule. Sepharose 45-54 cytochrome c, somatic Homo sapiens 0-12 6358361-1 1983 A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. Sepharose 164-171 macrophage migration inhibitory factor Homo sapiens 66-104 6358361-1 1983 A method is described for quantitating the biological activity of macrophage migration inhibitory factor (MIF) based on the detection of macrophages migrating from agarose droplets. Sepharose 164-171 macrophage migration inhibitory factor Homo sapiens 106-109 6415060-8 1983 Photoaffinity labeling of lactose synthase either free in solution or immobilized on Sepharose does not result in any reduction of the alpha-lactalbumin modifier activity. Sepharose 85-94 beta-1,4-galactosyltransferase 1 Homo sapiens 26-42 6605344-2 1983 A quantitative recovery of high Mr vWf oligomers has been obtained after binding to a monoclonal anti-vWf-Sepharose adduct. Sepharose 106-115 von Willebrand factor Homo sapiens 35-38 6605344-2 1983 A quantitative recovery of high Mr vWf oligomers has been obtained after binding to a monoclonal anti-vWf-Sepharose adduct. Sepharose 106-115 von Willebrand factor Homo sapiens 102-105 6685662-2 1983 This basically includes affinity purification on DNase-I agarose. Sepharose 57-64 deoxyribonuclease 1 Bos taurus 49-56 6226317-6 1983 Mucolipidosis I-excreted beta-hexosaminidase which was eluted from ricin-120-Sepharose, was a high-uptake form in endocytosis experiments while unbound enzyme was a low-uptake form. Sepharose 77-86 O-GlcNAcase Homo sapiens 25-44 6652064-5 1983 The major plasma membrane glycoprotein of the Ehrlich cell (GP 130) was isolated in near homogeneous form by using nonionic detergent extraction, affinity chromatography over GS I-Sepharose 4B, and preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 180-192 interleukin 6 signal transducer Mus musculus 60-66 6670741-8 1983 2-Trifluoromethyl-10-aminopropyl phenothiazine (TAPP), when linked to epoxide-activated Sepharose, was found to be the most useful for calmodulin isolation in terms of its combined stability, capacity, and ability to select for calmodulin. Sepharose 88-97 calmodulin 1 Homo sapiens 135-145 6360207-3 1983 The renin component was purified further by passage through an anti-rat spleen cathepsin D immunoglobulin G-Sepharose (IgG-Sepharose) column followed by carboxymethyl-Sephadex (CM-Sepharose) chromatography which separated two renin components. Sepharose 123-132 renin Rattus norvegicus 4-9 6360207-3 1983 The renin component was purified further by passage through an anti-rat spleen cathepsin D immunoglobulin G-Sepharose (IgG-Sepharose) column followed by carboxymethyl-Sephadex (CM-Sepharose) chromatography which separated two renin components. Sepharose 123-132 renin Rattus norvegicus 4-9 6360207-4 1983 Cathepsin D activity obtained by the fifth step was purified by passage through an anti-rat kidney renin IgG-Sepharose column followed by DEAE-Sephacel chromatography which separated three cathepsin D components. Sepharose 109-118 cathepsin D Rattus norvegicus 0-11 6605165-5 1983 In the propositus, analysis of vWF multimers in plasma on 1.6% sodium dodecyl sulfate (SDS) agarose revealed that there were no larger multimers and there was a relative increase of the smallest multimer. Sepharose 92-99 von Willebrand factor Homo sapiens 31-34 6414554-3 1983 In NaDodSO4-4% polyacrylamide/0.5% agarose gels (NaDodSO4-4% PAAGE), purified VIII:C had a major band of mol wt 1.0 X 10(5), with minor bands of mol wt 0.96, 1.1, 1.4, 1.6, 1.8, 2.2, and 2.4 X 10(5). Sepharose 35-42 cytochrome c oxidase subunit 8A Homo sapiens 78-82 6661462-1 1983 Fibronectin isolated from bovine serum by affinity chromatography on collagen-Sepharose was found to contain a great number of concomitant proteins. Sepharose 78-87 fibronectin 1 Homo sapiens 0-11 6642644-3 1983 Ab molecules were then eluted from Pn, and C3b-associated molecules were purified on avidin-Sepharose. Sepharose 92-101 complement C3 Homo sapiens 43-46 6642644-5 1983 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of dithiothreitol and NH2OH eluates from the avidin-Sepharose showed that C3b bound to both heavy and light chains of the Ab. Sepharose 110-119 complement C3 Homo sapiens 132-135 6355062-1 1983 Using blue Sepharose affinity chromatography, we purified orotidine-5"-phosphate decarboxylase over 600-fold, to near homogeneity, from strains of Escherichia coli harboring the cloned pyrF gene on the multicopy plasmid pDK26. Sepharose 11-20 orotidine-5'-phosphate decarboxylase Saccharomyces cerevisiae S288C 58-94 6194262-1 1983 Monospecific anti-rat serum alpha-fetoprotein (AFP) IgG was coupled to cyanogen bromide-activated Sepharose-4B (4.5 mg/ml packed volume of gel) to yield an immunoaffinity matrix. Sepharose 98-110 alpha-fetoprotein Rattus norvegicus 47-50 6605399-5 1983 Affinity depletion of synovial fluid fibronectin resulted in loss of C1q and reduction in IgG in the cryoprotein; however, fibronectin, C1q, and IgG could not be co-eluted from affinity matrices of gelatin and protein A-Sepharose. Sepharose 220-229 fibronectin 1 Homo sapiens 37-48 6631716-8 1983 Another MG antibody type which interferes with lectin binding to AChR (MG-C antibody) gave a 50% maximum inhibition of both HTP- and Lens culinaris agarose-AChR. Sepharose 148-155 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 65-69 6631716-8 1983 Another MG antibody type which interferes with lectin binding to AChR (MG-C antibody) gave a 50% maximum inhibition of both HTP- and Lens culinaris agarose-AChR. Sepharose 148-155 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 156-160 6643460-9 1983 The activation was absolutely dependent upon Factor Xa and was eliminated by immunoadsorption of the Factor Xa preparation with a murine anti-Factor X (Xa) monoclonal antibody coupled to agarose. Sepharose 187-194 coagulation factor X Homo sapiens 45-54 6643460-9 1983 The activation was absolutely dependent upon Factor Xa and was eliminated by immunoadsorption of the Factor Xa preparation with a murine anti-Factor X (Xa) monoclonal antibody coupled to agarose. Sepharose 187-194 coagulation factor X Homo sapiens 101-110 6626744-1 1983 Coagulation factor XI was purified from human plasma using ion-exchange chromatography and affinity chromatography on high molecular weight kininogen-Sepharose. Sepharose 150-159 coagulation factor XI Homo sapiens 0-21 6605390-2 1983 Partial purification of NAGO-TCGF on Sepharose G-100 columns gave a TCGF-active fraction within the same molecular weight range as the conventional lectin-induced TCGF (approximately 15,000 Da). Sepharose 37-46 interleukin 2 Homo sapiens 24-33 6626744-1 1983 Coagulation factor XI was purified from human plasma using ion-exchange chromatography and affinity chromatography on high molecular weight kininogen-Sepharose. Sepharose 150-159 kininogen 1 Homo sapiens 118-149 6226656-7 1983 The oligosaccharides of alpha 1-proteinase inhibitor secreted from 1-deoxynojirimycin-treated cells were characterized by their susceptibility to endoglucosaminidase H, incorporation of [3H]galactose, and [3H]fucose and concanavalin A-Sepharose chromatography. Sepharose 235-244 serpin family A member 1 Rattus norvegicus 24-52 6630199-1 1983 Serum spreading factor (SF) was isolated from human serum by a four-step procedure employing affinity chromatography on glass beads, concanavalin A-Sepharose, DEAE-agarose, and heparin-agarose. Sepharose 148-157 vitronectin Homo sapiens 0-27 6630199-1 1983 Serum spreading factor (SF) was isolated from human serum by a four-step procedure employing affinity chromatography on glass beads, concanavalin A-Sepharose, DEAE-agarose, and heparin-agarose. Sepharose 164-171 vitronectin Homo sapiens 0-27 6194158-7 1983 Additionally, it was found that transcriptionally active TFIIIA could be purified to greater than 95% homogeneity from ovarian extracts using an antibody coupled to Sepharose. Sepharose 165-174 general transcription factor 3A L homeolog Xenopus laevis 57-63 6196051-7 1983 The molecular weight of rat filaggrin mRNA was estimated to be 5 X 10(6) by electrophoresis in denaturing agarose gels containing methylmercury(II) hydroxide. Sepharose 106-113 filaggrin Rattus norvegicus 28-37 6660491-2 1983 The lectin is recovered from a PBE94 column in two peaks, each with a specific activity comparable to that of lectin purified by affinity chromatography on ovomucoid-Sepharose. Sepharose 166-175 LTL Solanum lycopersicum 4-10 6309826-1 1983 The insulin receptor purified from human placenta by sequential affinity chromatography on wheat germ agglutinin- and insulin-Sepharose to near homogeneity retained tyrosine-specific protein kinase activity. Sepharose 126-135 insulin receptor Homo sapiens 4-20 6606861-3 1983 Gel filtration chromatography (Sepharose 2B and 4B) of preincubated mixtures of C1q and Pl Fn yielded evidence of complex formation. Sepharose 31-40 complement C1q A chain Homo sapiens 80-83 6615885-1 1983 Cyclic nucleotide-independent and Ca2+-independent phosvitin kinase was purified from pig testis to apparent homogeneity by DEAE-cellulose, Sephadex G-200 chromatography, followed by subsequent DEAE-cellulose chromatography and phosvitin-Sepharose 4B affinity chromatography. Sepharose 238-247 casein kinase II subunit beta Sus scrofa 51-60 6639055-0 1983 The comparative kinetics of soluble and heparin-Sepharose-immobilized bovine lipoprotein lipase. Sepharose 48-57 lipoprotein lipase Bos taurus 77-95 6639055-2 1983 Hence kinetic parameters of S-LPL and heparin-Sepharose-immobilized LPL (B-LPL) were compared. Sepharose 46-55 lipoprotein lipase Bos taurus 68-71 6639055-2 1983 Hence kinetic parameters of S-LPL and heparin-Sepharose-immobilized LPL (B-LPL) were compared. Sepharose 46-55 lipoprotein lipase Bos taurus 73-78 6639055-10 1983 It is concluded that most of the LPL bound to heparin-Sepharose is probably inaccessible to substrate, hence a low Vmax. Sepharose 54-63 lipoprotein lipase Bos taurus 33-36 6579044-2 1983 Concanavalin A-induced LIF production was tested in an indirect LIF assay using agarose microdroplet technique; PGE1 and PGE2 inhibited the LIF production. Sepharose 80-87 LIF interleukin 6 family cytokine Homo sapiens 23-26 6579044-2 1983 Concanavalin A-induced LIF production was tested in an indirect LIF assay using agarose microdroplet technique; PGE1 and PGE2 inhibited the LIF production. Sepharose 80-87 LIF interleukin 6 family cytokine Homo sapiens 64-67 6579044-2 1983 Concanavalin A-induced LIF production was tested in an indirect LIF assay using agarose microdroplet technique; PGE1 and PGE2 inhibited the LIF production. Sepharose 80-87 LIF interleukin 6 family cytokine Homo sapiens 64-67 6194219-12 1983 This possibility was confirmed by showing binding of the receptor to an anti-IaK antibody-Sepharose affinity column. Sepharose 90-99 aurora kinase A Mus musculus 77-80 6310602-5 1983 Serial passaging of Bgl II fragment C-induced cultures and isolated clones resulted in the appearance of large (greater than 0.25 mm) colonies in agarose followed by tumorigenicity. Sepharose 146-153 LPS responsive beige-like anchor protein Homo sapiens 20-23 6309826-1 1983 The insulin receptor purified from human placenta by sequential affinity chromatography on wheat germ agglutinin- and insulin-Sepharose to near homogeneity retained tyrosine-specific protein kinase activity. Sepharose 126-135 insulin Homo sapiens 4-11 6605390-2 1983 Partial purification of NAGO-TCGF on Sepharose G-100 columns gave a TCGF-active fraction within the same molecular weight range as the conventional lectin-induced TCGF (approximately 15,000 Da). Sepharose 37-46 interleukin 2 Homo sapiens 29-33 6617876-3 1983 The interaction of microtubule proteins with S-100 protein was also demonstrated by Ca+-dependent affinity chromatography on S-100 protein-Sepharose. Sepharose 139-148 S100 calcium binding protein A1 Homo sapiens 45-50 6604056-4 1983 We also show that antibodies against eIF-4A react with a 50-kilodalton polypeptide present in a cap-binding protein complex obtained by elution from a m7GTP-agarose affinity column. Sepharose 157-164 eukaryotic initiation factor 4A-I Oryctolagus cuniculus 37-43 6617876-3 1983 The interaction of microtubule proteins with S-100 protein was also demonstrated by Ca+-dependent affinity chromatography on S-100 protein-Sepharose. Sepharose 139-148 S100 calcium binding protein A1 Homo sapiens 125-130 6309241-2 1983 The protein phosphatase activity has now been shown to be retained by a substrate affinity column (thiophosphorylated myosin P-light chain Sepharose) in the presence of Ca2+, and to be eluted specifically with EGTA. Sepharose 139-148 myosin heavy chain 14 Homo sapiens 118-124 6885784-2 1983 Novel features of the method include: 1) EDTA treatment of thylakoids prior to detergent extraction; 2) affinity chromatography over equine cytochrome c linked to Sepharose 4B; and 3) inclusion of the protease inhibitor phenylmethylsulfonyl fluoride. Sepharose 163-175 cytochrome c, somatic Equus caballus 140-152 6578848-3 1983 (1) Endo A bound to oxidized DNA-Sepharose under conditions where Endo B did not. Sepharose 33-42 endonuclease Escherichia coli 4-10 6356985-2 1983 Alcohol dehydrogenase was purified in 14 h from male Fischer-344 rat livers by differential centrifugation, (NH4)2SO4 precipitation, and chromatography over DEAE-Affi-Gel Blue, Affi-Gel Blue, and AMP-agarose. Sepharose 200-207 aldo-keto reductase family 1 member A1 Rattus norvegicus 0-21 6638506-7 1983 Despite the finding that BAL interacts with heparin-Sepharose, soluble heparin had no effect on BAL activity. Sepharose 52-61 carboxyl ester lipase Homo sapiens 25-28 6091698-4 1983 The synthesis of cytochrome-c-Sepharose was carried out in a way preventing modification of the lysine-containing binding domain of the cytochrome c molecule. Sepharose 30-39 LOC104968582 Bos taurus 136-148 6091699-1 1983 Preparations of horse heart cytochrome c have been obtained immobilized on Sepharose derivatives via lysine epsilon-amino groups, carboxyl groups of aspartic and glutamic acid residues, methionine and histidine residues as well as imidazole groups additionally introduced by means of chemical modification of free carboxyl groups by histamine. Sepharose 75-84 cytochrome c, somatic Equus caballus 28-40 6351865-4 1983 In addition to having the lowest efficiency with A134U, the bacterial ADA was also distinguished by its lack of binding of the mammalian ADA inhibitor erythro-9-(2-hydroxy-3-nonyl)adenine and by its weak binding to the 9-(p-aminobenzyl)adenine-agarose affinity column. Sepharose 244-251 adenosine deaminase Homo sapiens 70-73 6411111-0 1983 Electroblotting of factor VIII/von Willebrand factor multimers after electrophoresis in SDS-agarose gel, discontinuous buffer system. Sepharose 92-99 von Willebrand factor Homo sapiens 31-52 6885110-2 1983 The purified EPX is a highly basic glycoprotein and shows one band on sodium dodecyl sulphate polyacrylamide gel electrophoresis and on agarose electrophoresis. Sepharose 136-143 eosinophil peroxidase Homo sapiens 13-16 6885111-0 1983 Release of PAF by human polymorphonuclear leucocytes stimulated by immune complexes bound to Sepharose particles and human erythrocytes. Sepharose 93-102 PCNA clamp associated factor Homo sapiens 11-14 6313713-9 1983 Beside vimentin, desmin, the 68 X 10(3) Mr neurofilament triplet protein, the glial fibrillary acidic protein and cytokeratins also bound to arginine methylester Sepharose 4B in a salt-stable manner and could be eluted with arginine. Sepharose 162-174 desmin Homo sapiens 17-23 6313713-11 1983 Among the degradation products of vimentin produced by the specific, Ca2+-activated proteinase, only those with molecular weights higher than 40 X 10(3) bound to arginine methylester Sepharose 4B. Sepharose 183-192 vimentin Homo sapiens 34-42 6631249-2 1983 IgG specific for apoC-III was purified from goat antisera and bound to Sepharose. Sepharose 71-80 apolipoprotein C3 Homo sapiens 17-25 6226087-0 1983 Complement (C3)-receptor-mediated phagocytosis of agarose beads by mouse macrophages. Sepharose 50-57 complement component 3 Mus musculus 0-14 6226087-4 1983 Intracellular degradation of C3bi linked to agarose beads was also demonstrated by testing binding of monoclonal antibodies against human C3c, C3g and C3d to beads extracted from the cells after phagocytosis. Sepharose 44-51 Rap guanine nucleotide exchange factor 1 Homo sapiens 143-146 6226087-4 1983 Intracellular degradation of C3bi linked to agarose beads was also demonstrated by testing binding of monoclonal antibodies against human C3c, C3g and C3d to beads extracted from the cells after phagocytosis. Sepharose 44-51 endogenous retrovirus group K member 13 Homo sapiens 151-154 6649521-1 1983 Preparations of fibronectin from bovine blood serum, obtained by means of affinity chromatography on collagen-Sepharose, contained immunoglobulins and other proteins, concentration of which constituted 48 +/- 5%. Sepharose 110-119 fibronectin 1 Bos taurus 16-27 6411128-8 1983 Studies with DEAD-Sepharose columns, gel electrophoresis, and proteoliposome substrates demonstrated that apolipoprotein A-I from these mammalian species are similar in molecular charge and weight and are functionally similar in lecithin-cholesterol acyltransferase activation. Sepharose 18-27 apolipoprotein A1 Homo sapiens 106-124 6626504-3 1983 The length distribution agreed with a theory based on the assumption that thrombin releases the second A peptide more rapidly than the first, with a ratio of the release rates for the two peptides which is qualitatively in agreement with that deduced from oligomer size distributions obtained by agarose gel electrophoresis. Sepharose 296-303 coagulation factor II, thrombin Homo sapiens 74-82 6309774-4 1983 The extent of phosphorylation of insulin and somatomedin-C receptors was assessed by immunoprecipitating the wheat germ agglutinin Sepharose eluates with monoclonal antibodies specific for each receptor and analyzing the immunoprecipitates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 131-140 insulin Homo sapiens 33-40 6309774-4 1983 The extent of phosphorylation of insulin and somatomedin-C receptors was assessed by immunoprecipitating the wheat germ agglutinin Sepharose eluates with monoclonal antibodies specific for each receptor and analyzing the immunoprecipitates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 131-140 insulin like growth factor 1 Homo sapiens 45-58 6309774-7 1983 The beta-subunit of the somatomedin-C receptor was also phosphorylated when solubilized IM-9 cells that had been purified on wheat germ agglutinin Sepharose were incubated with [gamma-32P]ATP. Sepharose 147-156 insulin like growth factor 1 Homo sapiens 24-37 6309774-9 1983 Tyrosyl phosphorylation of the somatomedin-C receptor also occurred when highly purified receptor, prepared by wheat germ agglutinin Sepharose affinity chromatography followed by immunoprecipitation, was incubated with [gamma-32P]ATP. Sepharose 133-142 insulin like growth factor 1 Homo sapiens 31-44 6885772-3 1983 After digestion of this octasaccharide with alpha-L-iduronidase and N-acetylglucosamine-6-sulfate sulfatase, two fractions, with high and low affinity for antithrombin, respectively, were isolated by affinity chromatography on antithrombin-Sepharose. Sepharose 240-249 serpin family C member 1 Homo sapiens 155-167 6305481-6 1983 Furthermore, the molecular heterogeneity of hCG and hCG-subunits was examined by affinity chromatography on Concanavalin A (Con A)-sepharose. Sepharose 131-140 chorionic gonadotropin subunit beta 5 Homo sapiens 44-47 6313045-3 1983 The protein was purified by DEAE-Sephacel chromatography and two CaM-Sepharose affinity column steps. Sepharose 69-78 calmodulin Bos taurus 65-68 6313045-4 1983 The first CaM-Sepharose column was run in the presence of Ca2+; the second was run in the presence of chelator in excess of Ca2+. Sepharose 14-23 calmodulin Bos taurus 10-13 6191970-1 1983 Immunoaffinity adsorption techniques, utilizing specific antisera for hCG and its subunits bound to Sepharose 4B, have been employed to separate hCG alpha beta dimer and free subunits of hCG. Sepharose 100-112 chorionic gonadotropin subunit beta 5 Homo sapiens 70-73 6191970-1 1983 Immunoaffinity adsorption techniques, utilizing specific antisera for hCG and its subunits bound to Sepharose 4B, have been employed to separate hCG alpha beta dimer and free subunits of hCG. Sepharose 100-112 chorionic gonadotropin subunit beta 5 Homo sapiens 145-148 6191970-1 1983 Immunoaffinity adsorption techniques, utilizing specific antisera for hCG and its subunits bound to Sepharose 4B, have been employed to separate hCG alpha beta dimer and free subunits of hCG. Sepharose 100-112 chorionic gonadotropin subunit beta 5 Homo sapiens 145-148 6225655-6 1983 Actin-specific activities in the two fractions--estimated after isolation of the actin on small DNase I--Sepharose affinity columns--are similar after labelling for less than 1 h. Direct incorporation of newly-synthesized actin into filaments is suggested from these results. Sepharose 105-114 actin, beta Gallus gallus 0-5 6225655-6 1983 Actin-specific activities in the two fractions--estimated after isolation of the actin on small DNase I--Sepharose affinity columns--are similar after labelling for less than 1 h. Direct incorporation of newly-synthesized actin into filaments is suggested from these results. Sepharose 105-114 actin, beta Gallus gallus 222-227 6875557-2 1983 This matrix was synthesised by coupling the reversible cholinesterase inhibitor, edrophonium chloride, to epoxy-activated Sepharose. Sepharose 122-131 butyrylcholinesterase Homo sapiens 55-69 6875557-6 1983 The edrophonium-Sepharose matrix bound only one form of acetylcholinesterase. Sepharose 16-25 acetylcholinesterase (Cartwright blood group) Homo sapiens 56-76 6313075-6 1983 During chromatography of phosphodiesterase on calmodulin-Sepharose the enzyme was eluted from the column both in the presence of EGTA and palmitic acid. Sepharose 57-66 calmodulin 1 Homo sapiens 46-56 6307989-1 1983 An insulin-like growth factor II (IGF-II) receptor was purified from rat chondrosarcoma cells by Triton X-100 solubilization of a 100,000 X g membrane preparation and affinity chromatography on a multiplication-stimulating activity (MSA)-Sepharose column. Sepharose 238-247 insulin-like growth factor 2 Rattus norvegicus 34-40 6626600-4 1983 Antigen PS-2 was purified by affinity column chromatography consecutively with phenyl-Sepharose CL 4B, concanavalin Sepharose 4B, lysin-Sepharose and phytohemagglutinin-Sepharose. Sepharose 116-128 trefoil factor 1 Homo sapiens 8-12 6861148-1 1983 A glycoprotein (GP) which is immunochemically and biologically related to human prostatic acid phosphatase (PAP) has been isolated from human seminal plasma by ammonium sulfate precipitation followed by sequential concanavalin A:Sepharose 4B column, anion-exchange chromatography and gel filtration. Sepharose 229-241 acid phosphatase 3 Homo sapiens 80-106 6861148-1 1983 A glycoprotein (GP) which is immunochemically and biologically related to human prostatic acid phosphatase (PAP) has been isolated from human seminal plasma by ammonium sulfate precipitation followed by sequential concanavalin A:Sepharose 4B column, anion-exchange chromatography and gel filtration. Sepharose 229-241 acid phosphatase 3 Homo sapiens 108-111 6345047-7 1983 Immunoaffinity chromatography with renin-specific antibodies coupled to Sepharose provided a simple and specific method for isolation of high-molecular-weight renin. Sepharose 72-81 renin Canis lupus familiaris 159-164 6872206-3 1983 mCK-2 was detected by electrophoresis on agarose gel, with fluorometric scanning. Sepharose 41-48 casein kinase 2, alpha prime polypeptide Mus musculus 0-5 6309862-12 1983 Extracting ATR-gold complexes with Triton X after a 30-min chase at 22 degrees C and purifying them on Sepharose-transferrin indicated that the internalized complexes remained bound to the transferrin receptor during their intracellular processing. Sepharose 103-112 transferrin Homo sapiens 113-124 6309862-12 1983 Extracting ATR-gold complexes with Triton X after a 30-min chase at 22 degrees C and purifying them on Sepharose-transferrin indicated that the internalized complexes remained bound to the transferrin receptor during their intracellular processing. Sepharose 103-112 transferrin Homo sapiens 189-200 6192211-1 1983 Angiotensin-converting enzyme was solubilized with papain from a particulate fraction of rat brain and purified to apparent homogeneity by a procedure including DEAE-cellulose, hydroxylapatite, Sephadex G-200, Cys(Bzl)-Pro-Sepharose, and ricin-Sepharose chromatography. Sepharose 223-232 angiotensin I converting enzyme Rattus norvegicus 0-29 6223106-6 1983 The solubilized thrombin retained association with specific macromolecular fibrin derivatives as demonstrated by sedimentation analysis, electrophoretic co-migration, and partitioning in agarose gel. Sepharose 187-194 coagulation factor II, thrombin Homo sapiens 16-24 6353206-5 1983 These beta 2m-binding molecules exhibited size- and charge-homogeneity when separated by gel filtration (Sepharose 4B) and by ion-exchange chromatography (DEAE-cellulose), respectively. Sepharose 105-114 beta-2-microglobulin Homo sapiens 6-13 6192571-5 1983 Using protein-A-sepharose-purified fractions of IgG1 and IgG2, raised in the parental females we found the specificity of this extra IgG1 to be directed against the H-2Kb private determinants, in contrast to the IgG2 which was found to be specific for H-2Kb, H-2Db and I-Ab subregion alloantigens. Sepharose 16-25 LOC105243590 Mus musculus 48-52 6192571-5 1983 Using protein-A-sepharose-purified fractions of IgG1 and IgG2, raised in the parental females we found the specificity of this extra IgG1 to be directed against the H-2Kb private determinants, in contrast to the IgG2 which was found to be specific for H-2Kb, H-2Db and I-Ab subregion alloantigens. Sepharose 16-25 LOC105243590 Mus musculus 133-137 6192571-5 1983 Using protein-A-sepharose-purified fractions of IgG1 and IgG2, raised in the parental females we found the specificity of this extra IgG1 to be directed against the H-2Kb private determinants, in contrast to the IgG2 which was found to be specific for H-2Kb, H-2Db and I-Ab subregion alloantigens. Sepharose 16-25 histocompatibility 2, K1, K region Mus musculus 165-170 6224293-0 1983 Complement (C3)-receptor-mediated phagocytosis of agarose beads by mouse macrophages. Sepharose 50-57 complement component 3 Mus musculus 0-14 6305991-1 1983 The membrane receptor for insulin-like growth factor II (IGF II) has been purified to near homogeneity from rat placenta by chromatography of crude plasma membranes solubilized in Triton X-100 on agarose-immobilized IGF II. Sepharose 196-203 insulin-like growth factor 2 Rattus norvegicus 26-63 6305991-1 1983 The membrane receptor for insulin-like growth factor II (IGF II) has been purified to near homogeneity from rat placenta by chromatography of crude plasma membranes solubilized in Triton X-100 on agarose-immobilized IGF II. Sepharose 196-203 insulin-like growth factor 2 Rattus norvegicus 57-63 6636046-1 1983 A hereditary abnormal antithrombin III (AT-III) "Antithrombin III Toyama" was purified from the plasma of a patient with recurrent thrombophlebitis by a procedure involving barium chloride and ammonium sulfate fractionations, affinity chromatography on anti-AT-III-Sepharose gel, and DEAE-Sephadex chromatography. Sepharose 265-274 serpin family C member 1 Homo sapiens 22-38 6636046-1 1983 A hereditary abnormal antithrombin III (AT-III) "Antithrombin III Toyama" was purified from the plasma of a patient with recurrent thrombophlebitis by a procedure involving barium chloride and ammonium sulfate fractionations, affinity chromatography on anti-AT-III-Sepharose gel, and DEAE-Sephadex chromatography. Sepharose 265-274 serpin family C member 1 Homo sapiens 40-46 6636046-1 1983 A hereditary abnormal antithrombin III (AT-III) "Antithrombin III Toyama" was purified from the plasma of a patient with recurrent thrombophlebitis by a procedure involving barium chloride and ammonium sulfate fractionations, affinity chromatography on anti-AT-III-Sepharose gel, and DEAE-Sephadex chromatography. Sepharose 265-274 serpin family C member 1 Homo sapiens 49-65 6883723-2 1983 The method is based on the observation that PP5 will bind to heparin and employs a heparin-Sepharose column as a key step in the procedure. Sepharose 91-100 tissue factor pathway inhibitor 2 Homo sapiens 44-47 6863272-4 1983 Photooxidation of the activated NH2-terminal disulfide knot, which is derived from fibrin and contains the NH2-terminal binding domain, reduced the ability of this fragment to bind to fibrinogen-Sepharose conjugate. Sepharose 195-204 fibrinogen beta chain Homo sapiens 184-194 6626600-4 1983 Antigen PS-2 was purified by affinity column chromatography consecutively with phenyl-Sepharose CL 4B, concanavalin Sepharose 4B, lysin-Sepharose and phytohemagglutinin-Sepharose. Sepharose 86-95 trefoil factor 1 Homo sapiens 8-12 6626600-4 1983 Antigen PS-2 was purified by affinity column chromatography consecutively with phenyl-Sepharose CL 4B, concanavalin Sepharose 4B, lysin-Sepharose and phytohemagglutinin-Sepharose. Sepharose 116-125 trefoil factor 1 Homo sapiens 8-12 6408933-3 1983 Sepharose 4B chromatography of secreted [3H]-glucosamine-labeled glycoproteins revealed that indomethacin inhibited release of a high-molecular (greater than 10(6) daltons) mucin-type glycoprotein. Sepharose 0-9 mucin Canis lupus familiaris 173-178 6190519-4 1983 The formation of stable complexes between antibodies of each patient and VIII:CAg was demonstrated by protein-A-Sepharose adsorption. Sepharose 112-121 cytochrome c oxidase subunit 8A Homo sapiens 73-77 6206864-2 1983 Comparative studies between these two proteins and various metal chelate Sepharoses have shown that both PAPP-A and alpha 2M bind to the copper and zinc, but not to the calcium, manganese or magnesium chelate gels. Sepharose 73-83 pappalysin 1 Homo sapiens 105-111 6206864-2 1983 Comparative studies between these two proteins and various metal chelate Sepharoses have shown that both PAPP-A and alpha 2M bind to the copper and zinc, but not to the calcium, manganese or magnesium chelate gels. Sepharose 73-83 alpha-2-macroglobulin Homo sapiens 116-124 6206864-4 1983 Analysis of proteins bound to the zinc chelate Sepharose showed alpha 2M to have the highest affinity for the matrix, followed by PAPP-A and fibronectin, which coeluted, and then PZP. Sepharose 47-56 alpha-2-macroglobulin Homo sapiens 64-72 6137972-4 1983 Purification procedure for TH involved chromatographies with DEAE-Sephacel, Bio-Gel A-1.5 m, and heparin-Sepharose. Sepharose 105-114 tyrosine hydroxylase Rattus norvegicus 27-29 6863982-1 1983 Human epidermal calmodulin was purified by phenothiazine-Sepharose affinity chromatography. Sepharose 57-66 calmodulin 1 Homo sapiens 16-26 6313160-5 1983 We have observed that both receptors were adsorbed by affinity columns of insulin-agarose, in a manner that did not yield insulin receptor entirely free from the BSM receptor. Sepharose 82-89 insulin Homo sapiens 74-81 6886376-5 1983 Using anti-male MUP-1A serum, the Mup-1 gene was clearly detected on agarose gel electrophorograms of not only intact male but also intact female urine. Sepharose 69-76 major urinary protein 1 Rattus norvegicus 34-39 6225985-4 1983 MIF was assessed by the inhibition of adult mononuclear phagocyte cell migration under agarose; LIF was assessed by polymorphonuclear cell migration under agarose. Sepharose 87-94 macrophage migration inhibitory factor Homo sapiens 0-3 6193602-1 1983 Pentosan polysulphate (PPS, Hemoclar, MW 6,700) was observed to have a low affinity for ATIII-Sepharose eluting at 0.3M NaCl. Sepharose 94-103 serpin family C member 1 Homo sapiens 88-93 6622845-0 1983 Serum albumin values from healthy cattle, sheep and horses determined by the immediate bromocresol green reaction and by agarose gel electrophoresis. Sepharose 121-128 albumin Bos taurus 0-13 6134553-1 1983 Guanylate cyclase activity was purified to apparent homogeneity from rat liver (7700-fold) and bovine lung (8600-fold) soluble fractions by ammonium sulfate precipitation, DEAE-cellulose chromatography, agarose gel filtration and isoelectric focussing. Sepharose 203-210 guanylate cyclase Bos taurus 0-17 6870900-1 1983 Dihydropteridine reductase from human brain has been purified to homogeneity using a naphthaquinone affinity column followed by chromatography on a 5"-AMP-Sepharose column. Sepharose 155-164 quinoid dihydropteridine reductase Homo sapiens 0-26 6134723-1 1983 We have purified a novel membrane ATPase from Streptococcus faecalis by the following procedure: extraction of membranes with Triton X-100 followed by fractionation of the extract by successive DEAE-cellulose chromatography, hydroxylapatite chromatography and Cm-Sepharose chromatography. Sepharose 263-272 ATPase Enterococcus faecalis 34-40 6309217-1 1983 Cytochrome oxidase is purified from rat liver and beef heart by affinity chromatography on a matrix of horse cytochrome c-Sepharose 4B. Sepharose 122-134 cytochrome c, somatic Equus caballus 109-121 6309217-2 1983 The success of this procedure, which employs a matrix previously found ineffective with beef or yeast oxidase, is attributed to thorough dispersion of the enzyme with nonionic detergent and a low density of cross-linking between the lysine residues of cytochrome c and the cyanogen bromide activated Sepharose. Sepharose 300-309 cytochrome c, somatic Equus caballus 252-264 6863260-8 1983 Clusterin, which was found to contain 4.5% glucosamine, binds to concanavalin A-Sepharose and also to wheat germ agglutinin Sepharose. Sepharose 80-89 clusterin Rattus norvegicus 0-9 6863260-8 1983 Clusterin, which was found to contain 4.5% glucosamine, binds to concanavalin A-Sepharose and also to wheat germ agglutinin Sepharose. Sepharose 124-133 clusterin Rattus norvegicus 0-9 6133558-1 1983 The renaturation of free and Sepharose-immobilized D-amino-acid oxidase (D-amino-acid:oxygen oxidoreductase (deaminating), EC 1.4.3.3), after its denaturation with 6 M guanidine hydrochloride, was investigated. Sepharose 29-38 D-amino acid oxidase Homo sapiens 51-71 6304052-2 1983 Glucokinase partially purified by ion exchange chromatography on DEAE-Cibacron blue F3GA agarose was incubated for brief periods (1 or 3 min) with glucose anomers. Sepharose 89-96 glucokinase Rattus norvegicus 0-11 6406486-1 1983 A glycoprotein-enriched fraction derived from 3T3-L1 adipocyte membranes by Triton X-100 extraction and chromatography on wheat germ agglutinin-agarose contains an insulin-activable protein kinase that catalyzes the phosphorylation of tyrosine residues in proteins (Petruzzelli, L. M., Ganguly, S., Smith, C.J., Cobb, M. H., Rubin, C.S., and Rosen, O. M. (1982) Proc. Sepharose 144-151 insulin Homo sapiens 164-171 6849976-6 1983 In the second series of studies, fibrinogen-Sepharose was prepared and the binding of degradation products directly determined. Sepharose 44-53 fibrinogen beta chain Homo sapiens 33-43 6405777-0 1983 Fast migrating protein, immunochemically related to human factor VIII, studied by crossed immunoelectrophoresis in agarose. Sepharose 115-122 coagulation factor VIII Homo sapiens 58-69 6839017-1 1983 Two heparin-neutralizing proteins secreted by thrombin-stimulated platelets were purified to homogeneity by means of heparin-agarose affinity chromatography. Sepharose 125-132 coagulation factor II, thrombin Homo sapiens 46-54 6839021-2 1983 PF4 antigen isolated from "postheparin plasma" by adsorption on heparin-agarose and elution with 2.0 M NaCl and "authentic PF4" isolated from human platelets showed identical patterns of migration as determined by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Sepharose 72-79 platelet factor 4 Homo sapiens 0-3 6345005-5 1983 This MIF species constitutes a major form secreted by Mo cells; it adheres to Con A-Sepharose, is trypsin-resistant, and is denser than pure protein as determined by CsCl density gradient centrifugation. Sepharose 84-93 macrophage migration inhibitory factor Homo sapiens 5-8 6345005-10 1983 These larger MIF forms have a pI of 3.0 to 3.5 and of 4.6 to 5.2 and lack affinity to Con A Sepharose. Sepharose 92-101 macrophage migration inhibitory factor Homo sapiens 13-16 6573952-6 1983 This protein was identified as the transferrin receptor by affinity absorption of extracts of 125I-surface protein-labeled cells to transferrin-Sepharose beads. Sepharose 144-153 transferrin Homo sapiens 35-46 6573952-6 1983 This protein was identified as the transferrin receptor by affinity absorption of extracts of 125I-surface protein-labeled cells to transferrin-Sepharose beads. Sepharose 144-153 transferrin Homo sapiens 132-143 6303758-3 1983 For this purpose, pGH was digested with bovine thrombin conjugated to Sepharose. Sepharose 70-79 coagulation factor II, thrombin Bos taurus 47-55 6852371-3 1983 When purified from agarose gel and translated in a reticulocyte cell-free system, P25 mRNA yielded a 25-kD polypeptide (P25), identical to a 25-kD protein of the cocoon in terms of pI value and partial peptide mapping pattern. Sepharose 19-26 fibrohexamerin Bombyx mori 82-85 6222914-12 1983 Although stable as a crude extract, the activity of the rhodopsin-cleaving enzyme, as well as the bovine serum albumin (BSA)-cleaving enzyme (cathepsin-D) were rapidly lost upon purification by DEAE-Sephacel and pepstatin-Sepharose. Sepharose 222-231 rhodopsin Bos taurus 56-65 6222914-12 1983 Although stable as a crude extract, the activity of the rhodopsin-cleaving enzyme, as well as the bovine serum albumin (BSA)-cleaving enzyme (cathepsin-D) were rapidly lost upon purification by DEAE-Sephacel and pepstatin-Sepharose. Sepharose 222-231 cathepsin D Bos taurus 142-153 6884995-1 1983 The steroid-binding core of estradiol receptor was purified from pig uterus cytosol by a protocol consisting of (1) adsorption to heparin-sepharose, (2) enzymatic release of the receptor core, (3) DEAE-chromatography, (4) Sephadex G-150 filtration and (5) chromatography on heparin-sepharose. Sepharose 138-147 estrogen receptor 1 Sus scrofa 28-46 6884995-1 1983 The steroid-binding core of estradiol receptor was purified from pig uterus cytosol by a protocol consisting of (1) adsorption to heparin-sepharose, (2) enzymatic release of the receptor core, (3) DEAE-chromatography, (4) Sephadex G-150 filtration and (5) chromatography on heparin-sepharose. Sepharose 282-291 estrogen receptor 1 Sus scrofa 28-46 6190841-1 1983 Affinity chromatography over bilirubinagarose and sulfobromophthalein (BSP)-agarose was used to isolate two proteins, with high affinities for bilirubin and BSP, respectively, from Triton X-100-solubilized rat liver plasma membranes. Sepharose 38-45 integrin-binding sialoprotein Rattus norvegicus 143-160 6415800-1 1983 Fibronectin (FN) is a glycoprotein (disulfite-bonded dimer of 200 to 220 Kd submits) found in a soluble form in blood (concentration 250--500 microg/ml), it can be removed from it by cryoprecipitation and affinity chromatography on gelatin or heparin-agarose. Sepharose 251-258 fibronectin 1 Homo sapiens 0-11 6574482-4 1983 The time required for activation is consistent with that needed for insulin-dependent self-phosphorylation of the receptor present in eluates from wheat germ lectin-agarose columns and in preparations of affinity-purified placental receptor. Sepharose 165-172 insulin Homo sapiens 68-75 6405788-5 1983 The galactosyltransferase-concanavalin A complex was retained on an alpha-lactalbumin-Sepharose column in the presence of N-acetylglucosamine and manganese chloride and was eluted from the column in their absence. Sepharose 86-95 lactalbumin alpha Homo sapiens 68-85 6405788-6 1983 Galactosyltransferase immobilized onto a Con A-Sepharose was still active either in the presence or absence of alpha-lactalbumin. Sepharose 47-56 lactalbumin alpha Homo sapiens 111-128 6405788-7 1983 Lactose synthase activity was also observed when the galactosyltransferase-concanavalin A complex was assayed with alpha-lactalbumin immobilized on Sepharose. Sepharose 148-157 beta-1,4-galactosyltransferase 1 Homo sapiens 0-16 6405788-7 1983 Lactose synthase activity was also observed when the galactosyltransferase-concanavalin A complex was assayed with alpha-lactalbumin immobilized on Sepharose. Sepharose 148-157 lactalbumin alpha Homo sapiens 115-132 6847676-1 1983 The dye Procion Red HE3B immobilized on agarose and available as Matrex Gel Red A is shown to bind citrate synthase and succinate thiokinase from a number of diverse organisms. Sepharose 40-47 epididymal protein 3B Homo sapiens 20-24 6847676-1 1983 The dye Procion Red HE3B immobilized on agarose and available as Matrex Gel Red A is shown to bind citrate synthase and succinate thiokinase from a number of diverse organisms. Sepharose 40-47 citrate synthase Homo sapiens 99-115 6840089-1 1983 Human ceruloplasmin was attached to activated thiol-Sepharose via its thiol groups and was then digested with pepsin. Sepharose 52-61 ceruloplasmin Homo sapiens 6-19 6193728-1 1983 Human pregnancy-associated plasma protein A (PAPP-A) binds to heparin-Sepharose. Sepharose 70-79 pappalysin 1 Homo sapiens 6-43 6193728-1 1983 Human pregnancy-associated plasma protein A (PAPP-A) binds to heparin-Sepharose. Sepharose 70-79 pappalysin 1 Homo sapiens 45-51 6193728-4 1983 The remaining PAPP-A could be purified by negative affinity chromatography on anti-total human serum immobilized on agarose. Sepharose 116-123 pappalysin 1 Homo sapiens 14-20 6307278-1 1983 The calmodulin-dependent cyclic AMP phosphodiesterase and cyclic GMP phosphodiesterase (EC 3.1.4.17) activity of rat pancreas was purified 280-fold by affinity chromatography on calmodulin-Sepharose 4B. Sepharose 189-198 calmodulin 1 Rattus norvegicus 4-14 6347189-1 1983 A new affinity column for renin was prepared by coupling the isosteric peptide inhibitor of renin, H.77 (D-His-Pro-Phe-His-LeuR-Leu-Val-Tyr, where R is a reduced isosteric bond, -CH2-NH-), to activated 6-aminohexanoic acid-Sepharose 4B. Sepharose 223-232 renin Homo sapiens 26-31 6347189-1 1983 A new affinity column for renin was prepared by coupling the isosteric peptide inhibitor of renin, H.77 (D-His-Pro-Phe-His-LeuR-Leu-Val-Tyr, where R is a reduced isosteric bond, -CH2-NH-), to activated 6-aminohexanoic acid-Sepharose 4B. Sepharose 223-232 renin Homo sapiens 92-97 6341243-3 1983 LT-B was isolated by agarose affinity chromatography from cell lysates of recombinant clones expressing the B subunit. Sepharose 21-28 lymphotoxin beta Homo sapiens 0-4 6574521-2 1983 Determination of plasma lipoproteins by a sensitive agarose gel electrophoretic technique demonstrated: (a) in the patients with two prebeta bands an intensification of the fast prebeta lipoprotein fraction after growth hormone administration; and (b) in the patients with one prebeta band the appearance of a second prebeta band after growth hormone administration. Sepharose 52-59 growth hormone 1 Homo sapiens 213-227 6409085-2 1983 The protein from chicken egg white that inhibits cysteine proteinases, and has been named "cystatin", was purified by ovomucin precipitation, affinity chromatography on carboxymethylpapain-Sepharose and chromatofocusing. Sepharose 189-198 cystatin C Gallus gallus 91-99 6300232-9 1983 By using a combination of a standard 51Cr release and soft agarose single cell analysis assays, beta-endorphin increased both the number of E:T cell conjugates and the number of active killer cells among target-binding cells. Sepharose 59-66 proopiomelanocortin Homo sapiens 96-110 6303388-7 1983 The single translation product was shown to be GAPDH by (1) comigration with pure GAPDH on sodium dodecyl sulfate-polyacrylamide gels, (2) precipitation with specific anti-GAPDH antiserum, (3) cyanylation fingerprinting, and (4) AMP-agarose affinity chromatography. Sepharose 233-240 glyceraldehyde-3-phosphate dehydrogenase Gallus gallus 47-52 6830817-11 1983 Moreover, it is shown that only fumarase tetramers with all four subunits nitrated are unable to bind to a Sepharose-pyromellitic acid affinity column. Sepharose 107-116 fumarate hydratase Sus scrofa 32-40 6131891-3 1983 The mRNAs for cytochromes P-450c and P-450d can be completely separated by electrophoresis in denaturing agarose gels and have chain lengths of approximately 4000 and 2000 nucleotides, respectively. Sepharose 105-112 cytochrome P450, family 1, subfamily a, polypeptide 1 Rattus norvegicus 26-43 6298234-1 1983 The receptor for nerve growth factor (NGF) has been purified to near homogeneity from octylglucoside extracts of A875 melanoma cell membranes by the use of repetitive affinity chromatography on NGF-Sepharose. Sepharose 198-207 nerve growth factor Homo sapiens 17-36 6298234-1 1983 The receptor for nerve growth factor (NGF) has been purified to near homogeneity from octylglucoside extracts of A875 melanoma cell membranes by the use of repetitive affinity chromatography on NGF-Sepharose. Sepharose 198-207 nerve growth factor Homo sapiens 38-41 6298234-3 1983 Chromatography on two columns of NGF-Sepharose yielded a 1500-fold purification of the receptor, as assessed by 125I-NGF binding, and permitted recovery of 9% of the total binding activity in the soluble extract. Sepharose 37-46 nerve growth factor Homo sapiens 33-36 6298234-3 1983 Chromatography on two columns of NGF-Sepharose yielded a 1500-fold purification of the receptor, as assessed by 125I-NGF binding, and permitted recovery of 9% of the total binding activity in the soluble extract. Sepharose 37-46 nerve growth factor Homo sapiens 117-120 6338005-4 1983 Furthermore, orotate phosphoribosyltransferase and orotidylate decarboxylase from this parasite were resolved from each other by biospecific elution from blue Sepharose, indicating that the parasite enzymes do not exist as a bifunctional protein. Sepharose 159-168 uridine monophosphate synthetase Homo sapiens 13-46 6846840-11 1983 The diffusion coefficient for B-12 in 1% agarose gels was found to be 1.4 +/- 0.2 X 10(-6) cm2 sec-1. Sepharose 41-48 secretory blood group 1, pseudogene Homo sapiens 95-100 6223624-17 1983 Thrombin also affected the mobility of protein S on agarose-gel electrophoresis in the presence of Ca2+, suggesting a decreased affinity to Ca2+ of the cleaved form of protein S as compared with the undegraded molecule. Sepharose 52-59 coagulation factor II, thrombin Homo sapiens 0-8 6185329-3 1983 The migration position on agarose gels of prolactin-specific mRNA from human decidua-chorion is similar to that for mRNA from ovine pituitary, suggesting a similar sized mRNA coding for prolactin in these different tissues and species. Sepharose 26-33 prolactin Homo sapiens 42-51 6337949-1 1983 Completely inactive renin was isolated from normal human plasma by DEAE-Sepharose column chromatography and Blue-Sepharose column chromatography. Sepharose 72-81 renin Homo sapiens 20-25 6337949-1 1983 Completely inactive renin was isolated from normal human plasma by DEAE-Sepharose column chromatography and Blue-Sepharose column chromatography. Sepharose 113-122 renin Homo sapiens 20-25 6337953-2 1983 Some characteristics of the assay were 1) prorenin was activated by Sepharose-bound trypsin at 4 degrees C; 2) the concentration of activator was not critical provided that incubation was prolonged until renin activity had reached a plateau; and 3) this plateau was stable and had the same height as after maximal activation with acid, pepsin, plasmin or urokinase. Sepharose 68-77 renin Homo sapiens 45-50 6842082-8 1983 ApoE-deficient rat HDL, separated by heparin-Sepharose affinity chromatography also showed highly specific saturable binding to intestinal cells. Sepharose 45-54 apolipoprotein E Rattus norvegicus 0-4 6602941-4 1983 Fibronectin bound tightly to gelatin-Sepharose and C1q-Sepharose and this binding could be inhibited by gelatin but not by C1q. Sepharose 37-46 fibronectin 1 Homo sapiens 0-11 6602941-4 1983 Fibronectin bound tightly to gelatin-Sepharose and C1q-Sepharose and this binding could be inhibited by gelatin but not by C1q. Sepharose 55-64 fibronectin 1 Homo sapiens 0-11 6602941-4 1983 Fibronectin bound tightly to gelatin-Sepharose and C1q-Sepharose and this binding could be inhibited by gelatin but not by C1q. Sepharose 55-64 complement C1q A chain Homo sapiens 51-54 6687919-1 1983 Highly purified transferrin mRNA was isolated from rat liver using indirect immunoprecipitation of polysomes with antibodies to rat transferrin and poly(U)-sepharose chromatography. Sepharose 156-165 transferrin Rattus norvegicus 16-27 6572933-2 1983 Erythrocytes were surface labeled with 125I and solubilized in Nonidet P-40.HuE-C3bR was purified by using C3-Sepharose affinity chromatography and analyzed by autoradiography of NaDodSO4/polyacrylamide gels. Sepharose 110-119 complement C3b/C4b receptor 1 (Knops blood group) Homo sapiens 80-84 16593360-5 1983 Calmodulin-depleted fractions containing quinate:NAD(+) oxidoreductase were obtained by passage of the crude extracts through an affinity column of 2-chloro-10-(3-aminopropyl)phenothiazine coupled to Sepharose 4B. Sepharose 200-209 calmodulin Bos taurus 0-10 6885739-1 1983 Renin binding protein (RnBP) was purified from porcine kidney using pepstatin affinity column chromatography, DEAE-Sepharose column chromatography, gel filtration on Ultrogel-AcA 34, aminohexyl-Sepharose 4B column chromatography, and high performance liquid chromatography (HPLC) on TSK-gel G-3000 SW. Sepharose 115-124 renin binding protein Homo sapiens 0-21 6885739-1 1983 Renin binding protein (RnBP) was purified from porcine kidney using pepstatin affinity column chromatography, DEAE-Sepharose column chromatography, gel filtration on Ultrogel-AcA 34, aminohexyl-Sepharose 4B column chromatography, and high performance liquid chromatography (HPLC) on TSK-gel G-3000 SW. Sepharose 115-124 renin binding protein Homo sapiens 23-27 6885739-1 1983 Renin binding protein (RnBP) was purified from porcine kidney using pepstatin affinity column chromatography, DEAE-Sepharose column chromatography, gel filtration on Ultrogel-AcA 34, aminohexyl-Sepharose 4B column chromatography, and high performance liquid chromatography (HPLC) on TSK-gel G-3000 SW. Sepharose 194-203 renin binding protein Homo sapiens 0-21 6885739-1 1983 Renin binding protein (RnBP) was purified from porcine kidney using pepstatin affinity column chromatography, DEAE-Sepharose column chromatography, gel filtration on Ultrogel-AcA 34, aminohexyl-Sepharose 4B column chromatography, and high performance liquid chromatography (HPLC) on TSK-gel G-3000 SW. Sepharose 194-203 renin binding protein Homo sapiens 23-27 6875279-1 1983 C4 is composed of two tightly linked genes (C4A and C4B) lying within the major histocompatibility complex of chromosome 6 that can be demonstrated by agarose gel electrophoresis. Sepharose 151-158 complement C4B (Chido blood group) Homo sapiens 52-55 6403639-5 1983 A facile one-step, two-site IRMA using Sepharose-bound human anti-VIII:C and labeled monoclonal IgG was designed. Sepharose 39-48 cytochrome c oxidase subunit 8A Homo sapiens 66-70 6601148-6 1983 It appears to be different from both BCGF and IL 2 in that it can be separated from the former by isoelectric focusing and from the latter by phenyl-Sepharose chromatography. Sepharose 149-158 interleukin 2 Homo sapiens 46-50 6302680-9 1983 In order to identify the platelet fibrinogen receptor, A2A9 immobilized on agarose was used for affinity chromatography. Sepharose 75-82 fibrinogen beta chain Homo sapiens 34-44 6222469-0 1983 Complement (C3) receptor-mediated attachment of agarose beads to mouse peritoneal macrophages and human monocytes. Sepharose 48-55 complement component 3 Mus musculus 0-14 6222469-2 1983 By using isolated human complement factors C3, B and D, agarose beads were coated with C3b. Sepharose 56-63 complement C3 Homo sapiens 43-54 6222469-2 1983 By using isolated human complement factors C3, B and D, agarose beads were coated with C3b. Sepharose 56-63 complement C3 Homo sapiens 87-90 6222469-4 1983 Agarose beads coated with C3b or C3bi bound strongly to monocytes. Sepharose 0-7 complement C3 Homo sapiens 26-29 6222469-6 1983 Trypsinization of agarose beads coated with C3bi abolished the attachment of the beads to macrophages and monocytes, probably because of conversion of C3bi to C3d. Sepharose 18-25 endogenous retrovirus group K member 13 Homo sapiens 159-162 6187739-3 1983 The glutathione S-transferase subunits synthesized in vitro were purified from the translation products by affinity chromatography on S-hexylglutathione-linked Sepharose 6B columns. Sepharose 160-169 hematopoietic prostaglandin D synthase Rattus norvegicus 4-29 6833288-6 1983 Direct interaction was shown by chromatography of the receptor on RNase A Sepharose. Sepharose 74-83 ribonuclease A family member 1, pancreatic Rattus norvegicus 66-73 6833290-2 1983 Sepharose CL-6B column chromatography of the 125I-insulin-receptor complex obtained by both of the above procedures yielded a highly radioactive 140,000-Da complex which was dissociated into small peptides when subjected to SDS-polyacrylamide gel electrophoresis. Sepharose 0-9 insulin Homo sapiens 50-57 6833291-1 1983 Insulin receptor was purified 2400-fold with an overall yield of 40% from human placental membranes by affinity chromatography on wheat germ agglutinin-Sepharose and insulin-Sepharose. Sepharose 152-161 insulin receptor Homo sapiens 0-16 6833291-1 1983 Insulin receptor was purified 2400-fold with an overall yield of 40% from human placental membranes by affinity chromatography on wheat germ agglutinin-Sepharose and insulin-Sepharose. Sepharose 174-183 insulin receptor Homo sapiens 0-16 6833291-2 1983 The receptor was eluted from insulin-Sepharose using mild conditions, eliminating urea, so that it was stable and retained full insulin-binding activity. Sepharose 37-46 insulin Homo sapiens 29-36 6833291-2 1983 The receptor was eluted from insulin-Sepharose using mild conditions, eliminating urea, so that it was stable and retained full insulin-binding activity. Sepharose 37-46 insulin Homo sapiens 128-135 6833291-6 1983 In comparison, the receptor eluted from insulin-Sepharose with previously used conditions in the presence of urea resulted in maximum insulin binding of only 6 micrograms per mg of protein. Sepharose 48-57 insulin Homo sapiens 40-47 6833291-6 1983 In comparison, the receptor eluted from insulin-Sepharose with previously used conditions in the presence of urea resulted in maximum insulin binding of only 6 micrograms per mg of protein. Sepharose 48-57 insulin Homo sapiens 134-141 6553016-3 1983 Human salivary kallikrein was isolated from saliva using affinity chromatography on aprotinin-Sepharose and anti-human urinary kallikrein IgG-Sepharose followed by ion exchange chromatography on DEAE-Sepharose. Sepharose 94-103 kallikrein related peptidase 4 Homo sapiens 15-25 6825981-3 1983 The protein can be further characterized as an estrogen receptor by its binding to heparin-Sepharose. Sepharose 91-100 estrogen receptor 1 Homo sapiens 47-64 6403674-9 1983 The peak of this protein coincided with that of enzyme activity during DEAE-cellulose and calmodulin-Sepharose chromatography. Sepharose 101-110 calmodulin 1 Homo sapiens 90-100 6188161-1 1983 Highly purified human placental insulin receptors were obtained by sequential affinity chromatography on wheat germ agglutinin and insulin-agarose. Sepharose 139-146 insulin Homo sapiens 32-39 6188161-1 1983 Highly purified human placental insulin receptors were obtained by sequential affinity chromatography on wheat germ agglutinin and insulin-agarose. Sepharose 139-146 insulin Homo sapiens 131-138 6348922-6 1983 A third preparation of an apparently pure fibronectin has also been obtained by immuno-adsorption with a Sepharose 4B column to which has been coupled an anti-fibronectin monoclonal antibody. Sepharose 105-114 fibronectin 1 Homo sapiens 42-53 6130793-0 1983 Interaction of purified brush-border membrane aminopeptidase N and dipeptidyl peptidase IV with lectin-sepharose derivatives. Sepharose 103-112 alanyl aminopeptidase, membrane Rattus norvegicus 46-62 6130793-3 1983 Alternatively, a greater proportion of dipeptidyl peptidase IV (EC 3.4.14.-) was bound by these immobilized lectins with 50% of the enzyme binding to Con A-Sepharose. Sepharose 156-165 dipeptidylpeptidase 4 Rattus norvegicus 39-62 6130793-0 1983 Interaction of purified brush-border membrane aminopeptidase N and dipeptidyl peptidase IV with lectin-sepharose derivatives. Sepharose 103-112 dipeptidylpeptidase 4 Rattus norvegicus 67-90 6130793-2 1983 Aminopeptidase N (EC 3.4.11.2), which contains 20% carbohydrate by weight, was bound minimally (less than 30%) by columns of Con A-, RCAI- and WGA-Sepharose. Sepharose 147-156 alanyl aminopeptidase, membrane Rattus norvegicus 0-16 6839503-0 1983 Determination of a glycosyl subunit of human serum albumin by concanavalin A-sepharose. Sepharose 77-86 albumin Homo sapiens 51-58 6296146-2 1983 The majority of protein in a bovine brain extract that binds to a calmodulin-Sepharose affinity column also is observed to bind in a metal ion-independent manner to phenyl-Sepharose through hydrophobic interactions. Sepharose 77-86 calmodulin Bos taurus 66-76 6296146-6 1983 Comparison of the proteins which are bound to and eluted from phenyl- and calmodulin-Sepharose affinity columns indicates that chromatography involving calmodulin-Sepharose resembles hydrophobic interaction chromatography with charged ligands. Sepharose 85-94 calmodulin Bos taurus 74-84 6296146-6 1983 Comparison of the proteins which are bound to and eluted from phenyl- and calmodulin-Sepharose affinity columns indicates that chromatography involving calmodulin-Sepharose resembles hydrophobic interaction chromatography with charged ligands. Sepharose 85-94 calmodulin Bos taurus 152-162 6296146-6 1983 Comparison of the proteins which are bound to and eluted from phenyl- and calmodulin-Sepharose affinity columns indicates that chromatography involving calmodulin-Sepharose resembles hydrophobic interaction chromatography with charged ligands. Sepharose 163-172 calmodulin Bos taurus 74-84 6296146-6 1983 Comparison of the proteins which are bound to and eluted from phenyl- and calmodulin-Sepharose affinity columns indicates that chromatography involving calmodulin-Sepharose resembles hydrophobic interaction chromatography with charged ligands. Sepharose 163-172 calmodulin Bos taurus 152-162 6296151-2 1983 The first method involves affinity chromatography of the transferrin receptor with a transferrin-linked Sepharose 4B resin. Sepharose 104-116 transferrin Homo sapiens 85-96 6839503-3 1983 The glycosyl albumin bound to concanavalin-A Sepharose was homogeneous when examined by immunoelectrophoresis and sodium dodecyl-sulphate polyacrylamide electrophoresis, whereas it showed a microheterogeneity when tested by isoelectric focusing. Sepharose 45-54 albumin Homo sapiens 13-20 6301464-2 1983 Fibronectin synthesized by HUH6 Cl5 was purified by gelatin-Sepharose affinity chromatography and compared with human plasma fibronectin in respect to molecular weight, electrophoretic mobility and antigenicity. Sepharose 60-69 fibronectin 1 Homo sapiens 0-11 6823320-5 1983 These proteins separately bound to calmodulin-Sepharose at high Ca2+ concentrations. Sepharose 46-55 calmodulin 1 Homo sapiens 35-45 6822544-2 1983 Calmodulin was removed from brain cytosol by DEAE-52 chromatography or by affinity chromatography employing fluphenazine-Sepharose. Sepharose 121-130 calmodulin 1 Homo sapiens 0-10 6839489-3 1983 Bradykinin immunoreactivity in the extract eluted as a single peak from an immunoaffinity column of anti-bradykinin IgG bound to Sepharose identically to both bradykinin and kininogen showing immunoidentity of these substances. Sepharose 129-138 kininogen 1 Homo sapiens 0-10 6839489-3 1983 Bradykinin immunoreactivity in the extract eluted as a single peak from an immunoaffinity column of anti-bradykinin IgG bound to Sepharose identically to both bradykinin and kininogen showing immunoidentity of these substances. Sepharose 129-138 kininogen 1 Homo sapiens 105-115 6600420-0 1983 Isoelectric focusing in agarose: classification of genetic variants of alpha 1-antitrypsin. Sepharose 24-31 serpin family A member 1 Homo sapiens 71-90 6600420-3 1983 We have developed a method for isoelectric focusing in agarose for the classification of alpha 1-antitrypsin variants. Sepharose 55-62 serpin family A member 1 Homo sapiens 89-108 6600421-6 1983 Furthermore the cigarette-smoke-damaged alpha 1-antitrypsin had an increased electrophoretic mobility in plain agarose compared with the pure protein and was more susceptible to proteolytic damage by elastase. Sepharose 111-118 serpin family A member 1 Homo sapiens 40-59 6687357-1 1983 Myosin light chain kinase has been purified approximately 1700-fold from bovine thyroid gland, using Affigel blue column chromatography, ammonium sulfate fractionation (0-60% saturation), Sepharose 6B gel filtration, and calmodulin-Sepharose affinity column chromatography. Sepharose 188-197 myosin light chain kinase, smooth muscle Bos taurus 0-25 6192765-1 1983 Lectin affinities of AFP were analyzed using Con A sepharose chromatography and crossed immuno-affino-electrophoresis. Sepharose 51-60 alpha fetoprotein Homo sapiens 21-24 6341156-1 1983 Prostatic acid phosphatase (PAP) was purified from human seminal plasma by precipitation with ammonium sulfate and by serial chromatographies with concanavalin A-Sepharose, Sephadex G-100, and carboxymethyl-cellulose. Sepharose 162-171 acid phosphatase 3 Homo sapiens 0-26 6341156-1 1983 Prostatic acid phosphatase (PAP) was purified from human seminal plasma by precipitation with ammonium sulfate and by serial chromatographies with concanavalin A-Sepharose, Sephadex G-100, and carboxymethyl-cellulose. Sepharose 162-171 acid phosphatase 3 Homo sapiens 28-31 6840700-4 1983 Affinity chromatography showed that only a fraction of ceruloplasmin was bound to Lens culinaris or Lathyrus odoratus lectin-Sepharose, whereas nearly all the protein was bound to Canavalia ensiformis lectin-Sepharose. Sepharose 125-134 ceruloplasmin Homo sapiens 55-68 6133001-6 1983 By chromatography on Protein A Sepharose, IgG3 was obtained and it was found to contain no TSAb; activity was, however, recovered in the Protein A-retained fraction (i.e. IgG 1 + 2 + 4). Sepharose 31-40 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 42-46 6185522-5 1983 Removing PAPP-A-like material from seminal plasma by adsorbtion to heparin-Sepharose reduced the inhibitory effect of seminal plasma on phytohemagglutinin-induced lymphocyte transformation. Sepharose 75-84 pappalysin 1 Homo sapiens 9-15 6337126-3 1983 Fibronectin covalently linked to agarose beads bound radiolabeled lipoteichoic acid in the acylated form but not in the deacylated form. Sepharose 33-40 fibronectin 1 Homo sapiens 0-11 6841360-1 1983 Phospholipase A2 of rat liver mitochondria was purified approximately 1,400-fold by extraction with KCl, and chromatographies on a Sephadex G-75 column and a diacyl-glycerophosphocholine-Sepharose affinity column. Sepharose 187-196 phospholipase A2 group IB Rattus norvegicus 0-16 16578765-1 1983 Horse heart cytochrome c linked to Sepharose 4B is used to purify reaction centers from Rhodopseudomonas sphaeroides R-26. Sepharose 35-44 cytochrome c, somatic Equus caballus 12-24 6845286-0 1983 Antithrombin III-binding capacity of heparin-sepharose as a function of activation temperature and duration. Sepharose 45-54 serpin family C member 1 Homo sapiens 0-16 6294098-2 1983 Protein C inhibitor was isolated from human plasma using conventional chromatographic technique consisting of barium citrate adsorption, polyethylene glycol fractionation, DEAE-Sepharose CL-6B treatment, ammonium sulfate fractionation, dextran sulfate-agarose chromatography, gel filtration on ACA-44, and DEAE-Sephacel chromatography. Sepharose 252-259 serpin family A member 5 Homo sapiens 0-19 6838526-2 1983 Enzyme-linked immunosorbent assay (ELISA) and affinity chromatography of ovoinhibitor-coupled Sepharose 4B showed that about 25% of rabbit anti-ovomucoid antibody reacted with ovoinhibitor. Sepharose 94-103 serine peptidase inhibitor, Kazal type 5 Gallus gallus 73-85 6838526-2 1983 Enzyme-linked immunosorbent assay (ELISA) and affinity chromatography of ovoinhibitor-coupled Sepharose 4B showed that about 25% of rabbit anti-ovomucoid antibody reacted with ovoinhibitor. Sepharose 94-103 serine peptidase inhibitor, Kazal type 7 (putative) Gallus gallus 144-153 6848519-4 1983 Labeled fibronectin was isolated from the culture media by gelatin-Sepharose chromatography, from cell surfaces by urea extraction, and from intracellular locations by cell lysis followed by immunoprecipitation. Sepharose 67-76 fibronectin 1 Homo sapiens 8-19 6613726-1 1983 Calf thymus thymidylate synthase was purified to homogeneity using a pteroyltetraglutamyllysine Sepharose affinity column. Sepharose 96-105 thymidylate synthetase Bos taurus 12-32 6133482-0 1983 Purification of guinea pig liver transglutaminase using a phenylalanine-sepharose 4B affinity column. Sepharose 72-81 protein-glutamine gamma-glutamyltransferase 2 Cavia porcellus 33-49 6372556-0 1983 Genetic polymorphism of the vitamin D binding protein (Gc protein) in pig plasma determined by agarose isoelectrofocusing. Sepharose 95-102 GC vitamin D binding protein Sus scrofa 28-53 6372556-1 1983 Genetic polymorphism of the pig plasma vitamin D binding protein Gc was demonstrated by agarose isoelectrofocusing followed by either autoradiography or immunofixation with specific human Gc antiserum. Sepharose 88-95 GC vitamin D binding protein Sus scrofa 39-64 6677247-2 1983 Plasma fibronectin was purified on gelatin-sepharose with 1 M arginine elution and iodinated with 125I by the solid phase glycoluril method. Sepharose 43-52 fibronectin 1 Homo sapiens 7-18 6847606-1 1983 Goblet-cell mucin of rat small intestine was purified from mucosal scrapings by using centrifugation, Sepharose 4B and Sepharose 2B chromatography. Sepharose 102-114 solute carrier family 13 member 2 Rattus norvegicus 12-17 6847606-1 1983 Goblet-cell mucin of rat small intestine was purified from mucosal scrapings by using centrifugation, Sepharose 4B and Sepharose 2B chromatography. Sepharose 102-111 solute carrier family 13 member 2 Rattus norvegicus 12-17 6847606-2 1983 The mucin was applied in low concentrations (1 microgram/track) to slab gels containing 0.5% agarose/2% (w/v) polyacrylamide, and bands were detected after electrophoresis by silver stain or by fluorography of 3H-labelled mucin. Sepharose 93-100 solute carrier family 13 member 2 Rattus norvegicus 4-9 6184096-5 1983 Antithrombin-heparin cofactor, which eluted from heparin-agarose with buffer containing 2.0 M NaCl, was functionally and antigenically identified as antithrombin III. Sepharose 57-64 serpin family C member 1 Homo sapiens 149-165 6321157-2 1983 mRNA coding for transferrin receptor (TR) was enriched by polysome immuno-adsorbed chromatography with monospecific rabbit IgG and protein-A Sepharose. Sepharose 141-150 transferrin receptor Homo sapiens 16-36 6321157-2 1983 mRNA coding for transferrin receptor (TR) was enriched by polysome immuno-adsorbed chromatography with monospecific rabbit IgG and protein-A Sepharose. Sepharose 141-150 transferrin receptor Homo sapiens 38-40 11894930-9 1983 When whole labeled membranes were solubilized in neutral detergent and applied to lectin-Sepharose columns the Rho(D) polypeptide adsorbed to Ricinus communis lectin but not to wheat germ lectin or Lens culinaris lectin. Sepharose 89-98 ras homolog family member D Homo sapiens 111-117 6360678-1 1983 Rat liver glyoxalase II has been purified to homogeneity by a rapid, two-step procedure involving affinity chromatography on S-carbobenzoxyglutathione coupled to Sepharose 4B. Sepharose 162-171 hydroxyacyl glutathione hydrolase Rattus norvegicus 10-23 6873037-2 1983 The enzymes A1, A2 and B were separated from enzyme C by binding to concanavalin A-Sepharose. Sepharose 83-92 ATPase H+ transporting V0 subunit a2 Homo sapiens 16-24 6416953-2 1983 The reaction took place at 25 degrees C in 0.3% agarose with 16.7% pig serum against mouse IgA, and was evaluated on the basis of a relationship between the progress of the precipitin zone and the square root of time. Sepharose 48-55 immunoglobulin heavy constant alpha Mus musculus 91-94 6418640-2 1983 Gel filtration in Sephadex G-200 and affinity chromatography with rabbit anti-mouse IgG and protein A-Sepharose showed that the antibodies responsible for the immune serum lytic activity are exclusively located in Ig of IgG isotype, including IgG1, IgG2a, IgG2b and probably IgG3. Sepharose 102-111 immunoglobulin heavy chain (V7183 family) Mus musculus 220-223 6432681-5 1983 The purified anti-lambda anti-body, coupled to Sepharose, was mitogenic for lambda-bearing normal B cells and BCL1 cells, and in the presence of a supernatant from Concanavalin-A (Con A) stimulated these cells to secrete IgM lambda. Sepharose 47-56 cyclin D1 Mus musculus 110-114 6301526-0 1983 Affinity chromatography of brain cyclic nucleotide phosphodiesterase using 3-(2-pyridyldithio)propionyl-substituted calmodulin linked to thiol-sepharose. Sepharose 143-152 calmodulin 1 Homo sapiens 116-126 6301526-3 1983 PDP-CaM was covalently coupled to free thiol-Sepharose 4B through formation of a stable mixed disulfide bond for use in affinity chromatography. Sepharose 45-54 calmodulin 1 Homo sapiens 4-7 6301526-4 1983 The binding capacity of the disulfide-linked CaM-Sepharose for phosphodiesterase activity was proportional to substituent level up to 4 mg of CaM/mL of gel; the total capacity of the gel for binding phosphodiesterase was 4 times that of CNBr-coupled CaM-Sepharose. Sepharose 49-58 calmodulin 1 Homo sapiens 45-48 6301526-4 1983 The binding capacity of the disulfide-linked CaM-Sepharose for phosphodiesterase activity was proportional to substituent level up to 4 mg of CaM/mL of gel; the total capacity of the gel for binding phosphodiesterase was 4 times that of CNBr-coupled CaM-Sepharose. Sepharose 49-58 calmodulin 1 Homo sapiens 142-145 6301526-4 1983 The binding capacity of the disulfide-linked CaM-Sepharose for phosphodiesterase activity was proportional to substituent level up to 4 mg of CaM/mL of gel; the total capacity of the gel for binding phosphodiesterase was 4 times that of CNBr-coupled CaM-Sepharose. Sepharose 49-58 calmodulin 1 Homo sapiens 142-145 6301526-4 1983 The binding capacity of the disulfide-linked CaM-Sepharose for phosphodiesterase activity was proportional to substituent level up to 4 mg of CaM/mL of gel; the total capacity of the gel for binding phosphodiesterase was 4 times that of CNBr-coupled CaM-Sepharose. Sepharose 254-263 calmodulin 1 Homo sapiens 45-48 6301526-4 1983 The binding capacity of the disulfide-linked CaM-Sepharose for phosphodiesterase activity was proportional to substituent level up to 4 mg of CaM/mL of gel; the total capacity of the gel for binding phosphodiesterase was 4 times that of CNBr-coupled CaM-Sepharose. Sepharose 254-263 calmodulin 1 Homo sapiens 142-145 6301526-4 1983 The binding capacity of the disulfide-linked CaM-Sepharose for phosphodiesterase activity was proportional to substituent level up to 4 mg of CaM/mL of gel; the total capacity of the gel for binding phosphodiesterase was 4 times that of CNBr-coupled CaM-Sepharose. Sepharose 254-263 calmodulin 1 Homo sapiens 142-145 6601049-4 1983 The mobility of PI NADE is identical to PI M by both starch and agarose electrophoresis. Sepharose 64-71 brain expressed X-linked 3 Homo sapiens 19-23 6551387-1 1983 Plasma prekallikrein, a precursor protein of kallikrein [EC 3.4.21.8], was highly purified from porcine plasma by chromatography on a DEAE-Sephadex A-50 column, followed by rechromatography on a DEAE-Sephadex A-50 column, chromatography on a CM-Sephadex C-50 column and affinity chromatography on a p-aminobenzamidine-epsilon-aminocaproic acid-Sepharose 4B column. Sepharose 344-353 kallikrein B1 Bos taurus 0-20 6841326-2 1983 Calmodulin bound to the particulate fractions of the hepatoma and normal liver cells was purified to electrophoretic homogeneity by a simple procedure which involves solubilization of the particulate fractions with LIS, extraction of the solubilized solution with 37.5% phenol, gel filtration, and affinity chromatography on Fluphenazine-Sepharose. Sepharose 338-347 calmodulin 1 Rattus norvegicus 0-10 6848554-2 1983 Arylsulfatase B from human eosinophils was purified free of contaminating proteins by gel filtration and sequential affinity chromatography on Affi-Gel Blue and zinc chelate Sepharose. Sepharose 174-183 arylsulfatase B Homo sapiens 0-15 6885065-4 1983 After reaction of the antibody with the enzyme, beta-glucocerebrosidase was precipitated by addition of Protein A-Sepharose beads, and was detected on the beads by its enzymatic activity. Sepharose 114-123 glucosylceramidase beta Homo sapiens 48-71 6685738-1 1983 Calmodulin was purified from bovine testis and soybean seed, using affinity chromatography on (6-amino-hexyl)-5-chloro-1-naphthylene sulfonamide (W7)-Sepharose. Sepharose 150-159 calmodulin Bos taurus 0-10 6685738-3 1983 Although bovine and soybean calmodulins share affinity for W7-Sepharose, share biological activity, and have similar amino acid compositions, antisera raised against soybean calmodulin recognize animal calmodulins with 20,000-fold less avidity than they do plant calmodulins. Sepharose 62-71 calmodulin Bos taurus 28-38 6183355-8 1983 The solubilized receptors exhibited some rebinding to hIgE-Sepharose, and this rebinding could be inhibited by either human or rodent IgE but not by human IgG. Sepharose 59-68 immunoglobulin heavy constant epsilon Homo sapiens 54-58 6183355-8 1983 The solubilized receptors exhibited some rebinding to hIgE-Sepharose, and this rebinding could be inhibited by either human or rodent IgE but not by human IgG. Sepharose 59-68 immunoglobulin heavy constant epsilon Homo sapiens 55-58 6885935-6 1983 The Sepharose chromatogram revealed four radioactive peaks: peak 1 at column void volume; peak 2, Kav = 0.27; peak 3, Kav = 0.77; and peak 4, Kav = 1.09. Sepharose 4-13 pseudopodium-enriched atypical kinase 1 Rattus norvegicus 60-66 6834358-2 1983 Purification of plasma-opsonic fibronectin by affinity chromatography with gelatin-Sepharose revealed that although in vitro hepatic Kupffer cell phagocytosis was absolutely dependent upon the presence of fibronectin, the purified fibronectin evaluated in concentrations similar to that found in plasma (350-450 micrograms/ml) supported phagocytosis at a level two- to threefold less than that observed in whole plasma. Sepharose 83-92 fibronectin 1 Homo sapiens 31-42 6132362-3 1983 Early amniotic fluid contains GGT which binds mostly to Con A-Sepharose and shows alpha-mobility on cellulose acetate electrophoresis. Sepharose 62-71 inactive glutathione hydrolase 2 Homo sapiens 30-33 6139791-0 1983 Separation and quantitation of hepatoma-associated gamma-glutamyltransferase by affinity chromatography with Affi-Gel blue and Con A-Sepharose. Sepharose 133-142 gamma-glutamyltransferase light chain family member 3 Homo sapiens 51-76 6139791-1 1983 Isozymes of serum gamma-glutamyltransferase (GGT) in patients with hepatocellular carcinoma (HCC) and other liver diseases were separated into two groups by double-affinity column chromatography with Affi-Gel blue and Con A-Sepharose, one recovered in the unbound fraction and the other in the bound fraction. Sepharose 224-233 gamma-glutamyltransferase light chain family member 3 Homo sapiens 18-43 6142454-4 1983 ATP-citrate lyase was further purified by CoA-agarose affinity chromatography and fatty acid synthetase was purified on Bio-Gel A-1.5m. Sepharose 46-53 ATP citrate lyase Rattus norvegicus 0-17 6337376-1 1983 Glucokinase (ATP:D-glucose 6-phosphotransferase, EC 2.7.1.2) from rat islets of Langerhans was partially purified by chromatography on DEAE-Cibacron blue F3GA agarose. Sepharose 159-166 glucokinase Rattus norvegicus 0-11 6552675-1 1983 Human leukocyte elastase and cathepsin G were isolated from purulent sputum by a simple procedure involving chromatography on elastin-agarose. Sepharose 134-141 elastin Homo sapiens 126-133 6552675-2 1983 Salt extracts of sputum were prepared, treated with DNase, and the precipitate which formed extracted and applied to a column of soluble elastin-Sepharose 4B. Sepharose 145-154 elastin Homo sapiens 137-144 6856582-3 1983 This method, which involves affinity chromatography with heparin-Sepharose, ion exchange chromatography using DEAE-Sepharose, and Sephadex G-100 gel chromatography, results in purified, functionally active AT III with a 39% yield. Sepharose 65-74 antithrombin-III Cavia porcellus 206-212 6828455-4 1983 The polyacrylamide P-6 bead has a greater density of surface groups than the Sepharose bead, and this correlates to the greater density of transferrin receptors on erythroblasts. Sepharose 77-86 transferrin Homo sapiens 139-150 6868831-0 1983 [Detection of esterase D and glyoxalase I in human hair roots using agarose gel thin-layer electrophoresis]. Sepharose 68-75 esterase D Homo sapiens 14-24 6600764-2 1983 An assay for detection and quantitation of intra-cellular biosynthesis and secretion of C1q was then developed, using Sepharose beads covalently coated with goat monospecific anti-C1q IgG. Sepharose 118-127 complement C1q A chain Homo sapiens 88-91 6868831-0 1983 [Detection of esterase D and glyoxalase I in human hair roots using agarose gel thin-layer electrophoresis]. Sepharose 68-75 glyoxalase I Homo sapiens 29-41 7183949-1 1982 Choline acetyltransferase was purified from rat striata by several sequential column chromatographic separations on Sepharose 4B, hydroxylapatite, Sephadex G-150 and CM-Sephadex, and finally by elution of enzymatically active protein from gel slices. Sepharose 116-128 choline O-acetyltransferase Rattus norvegicus 0-25 6185152-2 1982 Fibronectin was purified from rat plasma by affinity chromatography on gelatin-Sepharose and arginine-Sepharose columns. Sepharose 79-88 fibronectin 1 Rattus norvegicus 0-11 6185152-2 1982 Fibronectin was purified from rat plasma by affinity chromatography on gelatin-Sepharose and arginine-Sepharose columns. Sepharose 102-111 fibronectin 1 Rattus norvegicus 0-11 6132465-4 1982 Studies on binding to Antithrombin III - Sepharose showed that sulphated guaran and a fraction of the aminated and sulphated alginic acid was bound, whereas no binding occurred with sulphated alginic acid. Sepharose 41-50 serpin family C member 1 Homo sapiens 22-38 7151801-1 1982 Prealbumin was purified from rat plasma by chromatography on Blue Sepharose CL-6B, followed by chromatography on concanavalin-A--Sepharose and preparative electrophoresis in polyacrylamide gel. Sepharose 66-75 transthyretin Oryctolagus cuniculus 0-10 7160472-0 1982 Interaction of fibronectin with arginine-agarose. Sepharose 41-48 fibronectin 1 Homo sapiens 15-26 6819859-1 1982 (LDH) obtained from larvae of Drosophila melanogaster was purified to homogeneity by affinity chromatography on oxamate-Sepharose. Sepharose 120-129 Lactate dehydrogenase Drosophila melanogaster 1-4 7165705-1 1982 Lipoprotein lipase (EC 3.1.1.34) extracted from adipose tissue of glucose-fed rats with 5 mM-sodium barbital, pH 7.5, containing 20% (v/v) glycerol and 0.1% (v/v) Triton X-100, was partially purified by affinity chromatography on heparin linked to Sepharose 4B. Sepharose 248-257 lipoprotein lipase Rattus norvegicus 0-18 7165705-11 1982 Purification of lipoprotein lipase from adipose tissue of glucose-fed rats was also carried out using affinity chromatography on Sepharose 4B linked to heparin with low affinity for antithrombin-III. Sepharose 129-141 lipoprotein lipase Rattus norvegicus 16-34 7165705-11 1982 Purification of lipoprotein lipase from adipose tissue of glucose-fed rats was also carried out using affinity chromatography on Sepharose 4B linked to heparin with low affinity for antithrombin-III. Sepharose 129-141 serpin family C member 1 Rattus norvegicus 182-198 7139922-2 1982 It was found to be very unstable at 37 degrees C under all these conditions, being transformed to CK-BB", a form that migrates like CK-MB on agarose electrophoretic plates. Sepharose 141-148 creatine kinase B Rattus norvegicus 98-104 6819138-3 1982 Lipoxygenase mRNA was enriched to about 75% by digestion of polysomes with protease K, poly(U)-Sepharose chromatography and repeated sucrose gradient centrifugation. Sepharose 95-104 polyunsaturated fatty acid lipoxygenase ALOX15 Oryctolagus cuniculus 0-12 6294139-5 1982 On the basis of its ability to form a complex with radiolabeled APC, the APC-binding protein was purified to homogeneity from normal human plasma by ammonium sulfate fractionation, heparin-agarose chromatography, and QAE-Sephadex A-50 chromatography. Sepharose 189-196 microtubule associated protein RP/EB family member 3 Homo sapiens 73-92 7161560-4 1982 The portion of apoE associated with triglyceride-rich lipoproteins as assessed by agarose column chromatography increased by a mean of 44%. Sepharose 82-89 apolipoprotein E Homo sapiens 15-19 7142179-6 1982 The Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase was found to bind to asialoprothrombin-agarose in the presence of CDP and could be eluted with a solution containing 0.2 M lactose and no CDP. Sepharose 98-106 cut-like homeobox 1 Rattus norvegicus 126-129 7142179-6 1982 The Gal beta 1 to 3(4)GlcNAc alpha 2 to 3 sialyltransferase was found to bind to asialoprothrombin-agarose in the presence of CDP and could be eluted with a solution containing 0.2 M lactose and no CDP. Sepharose 98-106 cut-like homeobox 1 Rattus norvegicus 198-201 6818950-10 1982 (3)H-labelled CAGA glycoprotein linked to Sepharose 4B failed to cause growth inhibition in CAGA-glycoprotein-sensitive cells. Sepharose 42-54 S100 calcium binding protein A8 Homo sapiens 14-18 7164033-4 1982 A heparin fraction with low affinity to antithrombin III was prepared from standard heparin by affinity chromatography on antithrombin-III-Sepharose and its properties compared with unfractionated heparin. Sepharose 139-148 serpin family C member 1 Homo sapiens 40-56 7164033-4 1982 A heparin fraction with low affinity to antithrombin III was prepared from standard heparin by affinity chromatography on antithrombin-III-Sepharose and its properties compared with unfractionated heparin. Sepharose 139-148 serpin family C member 1 Homo sapiens 122-138 6293233-1 1982 Radioimmunoassay using labelled somatostatin-14 revealed that components of somatostatin-28 antisera cross-reactive to somatostatin-14 were removed by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 195-207 somatostatin Homo sapiens 32-47 6293233-1 1982 Radioimmunoassay using labelled somatostatin-14 revealed that components of somatostatin-28 antisera cross-reactive to somatostatin-14 were removed by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 195-207 somatostatin Homo sapiens 76-91 6293233-1 1982 Radioimmunoassay using labelled somatostatin-14 revealed that components of somatostatin-28 antisera cross-reactive to somatostatin-14 were removed by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 195-207 somatostatin Homo sapiens 119-134 6293233-1 1982 Radioimmunoassay using labelled somatostatin-14 revealed that components of somatostatin-28 antisera cross-reactive to somatostatin-14 were removed by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 195-207 somatostatin Homo sapiens 165-180 6293233-1 1982 Radioimmunoassay using labelled somatostatin-14 revealed that components of somatostatin-28 antisera cross-reactive to somatostatin-14 were removed by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 195-207 somatostatin Homo sapiens 119-134 6293578-2 1982 Microsomal and lysosomal beta-glucuronidase (beta-D-glucuronide glucuronosohydrolase, EC 3.2.1.31) of monkey brain were differentially eluted from Con A-Sepharose when subjected to chromatography and linear gradient elution with methyl alpha-glucoside at 28+/-1 degree C. The lysosomal enzyme was eluted as a sharp peak in the first few fractions, while the microsomal enzyme was eluted as a broad peak extending over several fractions. Sepharose 153-162 glucuronidase beta Homo sapiens 25-43 6756543-2 1982 The inhibitor in the extract of cloned neuroblastoma cells was separated from renin activity by anti-renin IgG-Sepharose and selectively concentrated by adsorption to renin-agarose gel. Sepharose 111-120 renin Homo sapiens 78-83 6758841-6 1982 Treatment with a Sepharose-bound prothrombin antibody demonstrated that about 20-25% of the total carboxylated microsomal protein precursor pool consists of prothrombin precursors. Sepharose 17-26 coagulation factor II Rattus norvegicus 33-44 6758841-6 1982 Treatment with a Sepharose-bound prothrombin antibody demonstrated that about 20-25% of the total carboxylated microsomal protein precursor pool consists of prothrombin precursors. Sepharose 17-26 coagulation factor II Rattus norvegicus 157-168 7158775-0 1982 Preparation of three types of heparin-sepharose and their binding activities to thrombin and antithrombin III. Sepharose 38-47 coagulation factor II, thrombin Homo sapiens 80-88 7158775-0 1982 Preparation of three types of heparin-sepharose and their binding activities to thrombin and antithrombin III. Sepharose 38-47 serpin family C member 1 Homo sapiens 93-109 7139131-1 1982 The genetically determined polymorphism of the B subunit of the human coagulation factor XIII (FXIIIB) was investigated by an improved technique of immunofixation agarose gel electrophoresis (IAGE). Sepharose 163-170 coagulation factor XIII B chain Homo sapiens 95-101 6600402-0 1983 Isoelectric focusing of human von Willebrand factor in urea-agarose gels. Sepharose 60-67 von Willebrand factor Homo sapiens 30-51 6600402-1 1983 An analytical technique has been developed for the isoelectric focusing (IEF) of plasma von Willebrand factor (vWF) in agarose gels containing urea. Sepharose 119-126 von Willebrand factor Homo sapiens 88-109 6600402-1 1983 An analytical technique has been developed for the isoelectric focusing (IEF) of plasma von Willebrand factor (vWF) in agarose gels containing urea. Sepharose 119-126 von Willebrand factor Homo sapiens 111-114 6293876-1 1982 A glycogen synthase kinase that is completely dependent on Ca2+ and calmodulin has been identified in mammalian skeletal muscle, and purified approximately 3000-fold by chromatography on phosphocellulose and calmodulin--Sepharose. Sepharose 220-229 calmodulin 1 Homo sapiens 208-218 6130913-1 1982 Iminocyclophosphamide has been identified among the metabolites produced by incubation of cyclophosphamide with cytochrome P-450 mono-oxygenase immobilized on beaded Sepharose. Sepharose 166-175 cytochrome P450 family 20 subfamily A member 1 Homo sapiens 112-143 7141365-0 1982 Electrophoretic typing of glyoxalase I (GLO I) isoenzymes using a mixed starch/agarose gel. Sepharose 79-86 glyoxalase I Homo sapiens 26-38 6765521-2 1982 The development of serum IgE antibodies to human, porcine, and bovine insulins was assessed by a sepharose radioallergoabsorbent test (RAST). Sepharose 97-106 immunoglobulin heavy constant epsilon Homo sapiens 25-28 6816595-4 1982 The activation of progastricsin at pH 2 was investigated and monitored by agarose gel electrophoresis at pH 5.4. Sepharose 74-81 progastricsin Homo sapiens 18-31 7141365-0 1982 Electrophoretic typing of glyoxalase I (GLO I) isoenzymes using a mixed starch/agarose gel. Sepharose 79-86 glyoxalase I Homo sapiens 40-45 7141365-1 1982 A technique was developed to type the glyoxalase I (GLO I) isoenzymes using a mixed agarose/starch gel. Sepharose 84-91 glyoxalase I Homo sapiens 38-50 6219044-2 1982 It was found that individuals with Lp(a) levels exceeding 30 mg/dl exhibited an extra pre-beta-band in agarose gel electrophoresis (sinking pre-beta positive), and a significant positive correlation existed between serum Lp(a) levels and age. Sepharose 103-110 lipoprotein(a) Homo sapiens 35-40 6219044-2 1982 It was found that individuals with Lp(a) levels exceeding 30 mg/dl exhibited an extra pre-beta-band in agarose gel electrophoresis (sinking pre-beta positive), and a significant positive correlation existed between serum Lp(a) levels and age. Sepharose 103-110 lipoprotein(a) Homo sapiens 221-226 7141365-1 1982 A technique was developed to type the glyoxalase I (GLO I) isoenzymes using a mixed agarose/starch gel. Sepharose 84-91 glyoxalase I Homo sapiens 52-57 6815213-4 1982 A population of multimeric FVIII/vWF species ranging in molecular weight from 1 to 5 million daltons and differing in size alternately by one and two subunits was observed on SDS-2% polyacrylamide-0.5% agarose gel electrophoresis. Sepharose 202-209 coagulation factor VIII Homo sapiens 27-32 6296267-1 1982 The binding of [125I]CEA to an anti-CEA Sepharose-4B immunosorbent has been investigated in the presence of detergents. Sepharose 40-52 CEA cell adhesion molecule 3 Homo sapiens 21-24 6296267-1 1982 The binding of [125I]CEA to an anti-CEA Sepharose-4B immunosorbent has been investigated in the presence of detergents. Sepharose 40-52 CEA cell adhesion molecule 3 Homo sapiens 36-39 6815213-4 1982 A population of multimeric FVIII/vWF species ranging in molecular weight from 1 to 5 million daltons and differing in size alternately by one and two subunits was observed on SDS-2% polyacrylamide-0.5% agarose gel electrophoresis. Sepharose 202-209 von Willebrand factor Homo sapiens 33-36 6815213-5 1982 With progressive reduction of disulfide bonds by dithiothreitol (DTT), the electron microscopic size of FVIII/vWF decreased in parallel with increased electrophoretic mobility on SDS-agarose gels; between 0.1 and 0.5 mM DTT its structure changed from predominantly fibrillar species to large nodular forms. Sepharose 183-190 coagulation factor VIII Homo sapiens 104-109 6754880-6 1982 In addition, we found that the binding of LHRH granules to Con A-Sepharose has a requirement for Ca2+ and Mn2+: after equilibration of Con A-Sepharose with Ca2+ and Mn2+ (prior to the chromatography), all of the LHRH granules were bound to the columns, and this binding was prevented by EDTA. Sepharose 65-74 gonadotropin releasing hormone 1 Rattus norvegicus 42-46 6982283-1 1982 A variant of von Willebrand"s disease has been identified in which sodium dodecyl sulfate agarose electrophoresis provides evidence that the von Willebrand factor present is structurally abnormal. Sepharose 90-97 von Willebrand factor Homo sapiens 141-162 6754880-6 1982 In addition, we found that the binding of LHRH granules to Con A-Sepharose has a requirement for Ca2+ and Mn2+: after equilibration of Con A-Sepharose with Ca2+ and Mn2+ (prior to the chromatography), all of the LHRH granules were bound to the columns, and this binding was prevented by EDTA. Sepharose 65-74 gonadotropin releasing hormone 1 Rattus norvegicus 212-216 6754880-6 1982 In addition, we found that the binding of LHRH granules to Con A-Sepharose has a requirement for Ca2+ and Mn2+: after equilibration of Con A-Sepharose with Ca2+ and Mn2+ (prior to the chromatography), all of the LHRH granules were bound to the columns, and this binding was prevented by EDTA. Sepharose 141-150 gonadotropin releasing hormone 1 Rattus norvegicus 42-46 6754880-7 1982 To examine the specificity of the binding of LHRH granules to Con A-Sepharose, a competing sugar (alpha-methyl-D-mannoside (alpha-MM)) was added to the LHRH granules, and the columns were equilibrated with alpha-MM prior to chromatography. Sepharose 68-77 gonadotropin releasing hormone 1 Rattus norvegicus 45-49 6754880-8 1982 Under this condition, the Ca2+- and Mn2+-dependent binding of LHRH granules to Con A-Sepharose was inhibited. Sepharose 85-94 gonadotropin releasing hormone 1 Rattus norvegicus 62-66 7175376-7 1982 Compared with the adult rats, a shift of apoA-IV in the HDL to the "lipoprotein-free" fraction was observed in the old rats, as measured by agarose gel chromatography. Sepharose 140-147 apolipoprotein A4 Rattus norvegicus 41-48 6754880-9 1982 In addition to Con A-Sepharose, we observed that LHRH granules bind to wheat germ agglutinin-Sepharose but not to Sepharose which does not contain a lectin. Sepharose 93-102 gonadotropin releasing hormone 1 Rattus norvegicus 49-53 6754880-9 1982 In addition to Con A-Sepharose, we observed that LHRH granules bind to wheat germ agglutinin-Sepharose but not to Sepharose which does not contain a lectin. Sepharose 93-102 gonadotropin releasing hormone 1 Rattus norvegicus 49-53 6294652-5 1982 The insulin-activated kinase is copurified with the human placental insulin receptor until the final elution from insulin-Sepharose. Sepharose 122-131 insulin Homo sapiens 4-11 7176651-1 1982 By the use of aqueous two-phase partitioning and DNA-Sepharose chromatography, Li+ has been found to cause a partial inhibition of the activation of the rat liver glucocorticoid receptor in vitro. Sepharose 53-62 nuclear receptor subfamily 3, group C, member 1 Rattus norvegicus 163-186 6294652-5 1982 The insulin-activated kinase is copurified with the human placental insulin receptor until the final elution from insulin-Sepharose. Sepharose 122-131 insulin receptor Homo sapiens 68-84 6292906-6 1982 Lysates (or Sepharose 6B ribosomes) treated with ds RNA display a similar ds I phosphoprotein profile, and this is accompanied by the phosphorylation of endogenous eIF-2alpha (38,000 daltons). Sepharose 12-21 eukaryotic translation initiation factor 2A Homo sapiens 164-174 6294652-5 1982 The insulin-activated kinase is copurified with the human placental insulin receptor until the final elution from insulin-Sepharose. Sepharose 122-131 insulin Homo sapiens 68-75 16662704-6 1982 DOVA transaminase from either wild-type or aplastidic derivative strain W(14)ZNaIL ran as a single band in agarose gel permeation chromatography, with a calculated molecular weight of 98,000 +/- 3,000. Sepharose 107-114 RAN, member RAS oncogene family Homo sapiens 6-9 6959134-1 1982 Anti-human fibronectin antibodies produced in a goat or in rabbits stimulate the release of serotonin from washed or gelatin/Sepharose-treated human platelets in a dose-dependent manner. Sepharose 125-134 fibronectin Capra hircus 11-22 6811587-2 1982 Chromatography of electrophoretically homogeneous cytochrome P-450LM4 from cholestyramine-treated rabbits on octylamine-Sepharose resulted in the isolation of two subfractions, cytochrome P-450LM4 I and cytochrome P-450LM4 II, with different catalytic properties. Sepharose 120-129 cytochrome P450 1A2 Oryctolagus cuniculus 50-69 7118896-3 1982 A yield of 46% and a 33,000-fold purification have been achieved, using poly-L-lysine-Sepharose affinity column chromatography, PF4-agarose affinity column chromatography, and Bio-Gel A-0.5m gel filtration. Sepharose 132-139 platelet factor 4 Homo sapiens 128-131 6982949-3 1982 In comparison it has been found that the anionic dyes Cibracon blue F3GA and Procion red HE3B, bound to crosslinked agarose, give more than 80% recovery and purification of DNA binding activity from whole SLE serum of 11- and 7-fold respectively. Sepharose 116-123 epididymal protein 3B Homo sapiens 89-93 7159489-6 1982 In studies with lipoproteins linked to Sepharose 4B, LCAT was found to bind LDL, HDL2, and HDL3. Sepharose 39-48 junctophilin 3 Homo sapiens 81-85 7159489-6 1982 In studies with lipoproteins linked to Sepharose 4B, LCAT was found to bind LDL, HDL2, and HDL3. Sepharose 39-48 HDL3 Homo sapiens 91-95 6890955-5 1982 SK-potentiator showed a similar mobility to that of fibrinogen on both SDS-polyacrylamide gel and agarose gel electrophoresis, and cross-reacted with anti-fibrinogen antiserum. Sepharose 98-105 fibrinogen beta chain Homo sapiens 52-62 7119782-1 1982 Dopamine beta-hydroxylase (DBH) was purified from bovine brain by a series of steps including extraction with 0.5% Triton X-100, ammonium sulfate fractionation, and serial chromatographies with Concanavalin A (Con A)-Sepharose, Biogel A-1.5 m, DEAE-Sephadex, and phenyl-Sepharose. Sepharose 217-226 dopamine beta-hydroxylase Bos taurus 0-25 7119782-1 1982 Dopamine beta-hydroxylase (DBH) was purified from bovine brain by a series of steps including extraction with 0.5% Triton X-100, ammonium sulfate fractionation, and serial chromatographies with Concanavalin A (Con A)-Sepharose, Biogel A-1.5 m, DEAE-Sephadex, and phenyl-Sepharose. Sepharose 217-226 dopamine beta-hydroxylase Bos taurus 27-30 7157225-0 1982 Isolation of platelet glycocalicin by affinity chromatography on thrombin-sepharose. Sepharose 74-83 coagulation factor II, thrombin Homo sapiens 65-73 6983840-0 1982 An improved method for phenotyping of alpha 1-antitrypsin variants using separator isoelectric focusing on thin-layer agarose gel. Sepharose 118-125 serpin family A member 1 Homo sapiens 38-57 7150233-0 1982 A general method for affinity purification of complement component C3b using factor H-sepharose. Sepharose 86-95 complement C3 Homo sapiens 67-70 7150233-0 1982 A general method for affinity purification of complement component C3b using factor H-sepharose. Sepharose 86-95 complement factor H Homo sapiens 77-85 7150233-1 1982 Complement component C3b has been purified from human, rabbit and bovine serum by affinity chromatography on human factor H-Sepharose after preliminary fractionation by poly(ethylene glycol) and DEAE-Sepharose. Sepharose 124-133 complement C3 Homo sapiens 0-24 7150233-1 1982 Complement component C3b has been purified from human, rabbit and bovine serum by affinity chromatography on human factor H-Sepharose after preliminary fractionation by poly(ethylene glycol) and DEAE-Sepharose. Sepharose 124-133 complement factor H Homo sapiens 115-123 7150233-3 1982 Binding of C3b to factor H-Sepharose is equimolar, has a sharp optimum pH at 7.6 and is quite sensitive to ionic strength. Sepharose 27-36 complement C3 Homo sapiens 11-14 7150233-3 1982 Binding of C3b to factor H-Sepharose is equimolar, has a sharp optimum pH at 7.6 and is quite sensitive to ionic strength. Sepharose 27-36 complement factor H Homo sapiens 18-26 7159570-3 1982 The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol hydrolysis and PNPB hydrolysis activities coelute from the heparin-Sepharose affinity column used to purify LpL and (b) LpL-catalyzed hydrolyses of trioleoylglycerol and PNPB are inhibited to equal extents by phenylmethanesulfonyl fluoride. Sepharose 231-240 lipoprotein lipase Bos taurus 272-275 7159570-3 1982 The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol hydrolysis and PNPB hydrolysis activities coelute from the heparin-Sepharose affinity column used to purify LpL and (b) LpL-catalyzed hydrolyses of trioleoylglycerol and PNPB are inhibited to equal extents by phenylmethanesulfonyl fluoride. Sepharose 231-240 lipoprotein lipase Bos taurus 284-287 6817663-0 1982 Purification of ferredoxin-NADP+ oxidoreductase from cyanobacteria by affinity chromatography on 2",5"-ADP-sepharose 4B. Sepharose 107-116 thioredoxin reductase 1 Homo sapiens 33-47 6756410-2 1982 To gain insight into the mechanism of these effects, we measured the oriented PMN migration under agarose induced, in the presence and absence of PBZ, by FMLP, zymosan-activated serum and Klebsiella pneumoniae culture supernatant. Sepharose 98-105 formyl peptide receptor 1 Homo sapiens 154-158 7159489-6 1982 In studies with lipoproteins linked to Sepharose 4B, LCAT was found to bind LDL, HDL2, and HDL3. Sepharose 39-48 lecithin-cholesterol acyltransferase Homo sapiens 53-57 6213631-2 1982 Additional extensive purification was accomplished by immunoaffinity chromatography on antihuman renin immunoglobulin G-Sepharose. Sepharose 120-129 renin Homo sapiens 97-102 6959991-6 1982 From its strong affinity toward concanavalin A-Sepharose and colorimetric determination of neutral sugars by the phenol-sulfuric acid method, arylsulfatase C was identified as a glycoprotein. Sepharose 47-56 steroid sulfatase Rattus norvegicus 142-157 7178043-1 1982 Particle-bound aminopeptidase was purified from the human term placenta by separation of the particles from the soluble fraction by centrifugation and solubilization of the particle fraction with 0.5 per cent (v/v) Triton X-100 followed by CM-cellulose chromatography, Sepharose 6B gel filtration, DE-cellulose and Con A-Sepharose 4B affinity chromatography. Sepharose 321-333 carboxypeptidase Q Homo sapiens 15-29 7157225-1 1982 Platelet glycocalicin has been purified to homogeneity by a two step procedure involving affinity chromatography on WGA-Sepharose and then on thrombin-Sepharose using selective elution with heparin. Sepharose 151-160 coagulation factor II, thrombin Homo sapiens 142-150 6809752-3 1982 The HDL3 fraction purified from the AI Milano subjects eluted as a symmetrical peak from a 6% agarose column, corresponding to a unimodal particle size distribution. Sepharose 94-101 HDL3 Homo sapiens 4-8 7050289-5 1982 The capacity of C3a(70-77)-Sepharose,m but not Sepharose alone, to adsorb or inactivate mononuclear leukocytes required for the generation of LIF activity established a direct interaction. Sepharose 27-36 complement C3 Homo sapiens 16-19 7050289-5 1982 The capacity of C3a(70-77)-Sepharose,m but not Sepharose alone, to adsorb or inactivate mononuclear leukocytes required for the generation of LIF activity established a direct interaction. Sepharose 27-36 LIF interleukin 6 family cytokine Homo sapiens 142-145 7050289-6 1982 Analysis of the lymphocytes in the effluent from C3a(70-77)-Sepharose columns, using monoclonal antibodies to surface antigens, showed a selective depletion of the helper/inducer population of lymphocytes. Sepharose 60-69 complement C3 Homo sapiens 49-52 6294899-2 1982 The platelet surface protein GP Ib (glycocalicin-related protein) has been shown to be retarded by thrombin-Sepharose 4B in a crossed immunoelectrophoresis system. Sepharose 108-120 coagulation factor II, thrombin Homo sapiens 99-107 7107587-12 1982 Affinity chromatography of the glycopeptides on concanavalin A-Sepharose also showed that the glycopeptides from fibrinogen are greater than 95% biantennary oligosaccharide chains. Sepharose 63-72 fibrinogen beta chain Homo sapiens 113-123 6182105-1 1982 Mouse monoclonal antibody directed against human leukocyte alpha interferon (IFN-alpha) was coupled to Sepharose and used as an immunoadsorbent to purify human IFN-alpha. Sepharose 103-112 interferon alpha 1 Homo sapiens 49-86 6182105-1 1982 Mouse monoclonal antibody directed against human leukocyte alpha interferon (IFN-alpha) was coupled to Sepharose and used as an immunoadsorbent to purify human IFN-alpha. Sepharose 103-112 interferon alpha 1 Homo sapiens 77-86 6125505-9 1982 Moreover, after incubation with the active fraction, tyrosine hydroxylase was bound to protein A-Sepharose and the activity recovered was severalfold higher than the initial activity. Sepharose 97-106 tyrosine hydroxylase Rattus norvegicus 53-73 7135347-0 1982 On the reliability of the use of heparin immobilized on agarose for the study of the interactions among heparin, thrombin and antithrombin. Sepharose 56-63 coagulation factor II, thrombin Homo sapiens 113-121 7135347-0 1982 On the reliability of the use of heparin immobilized on agarose for the study of the interactions among heparin, thrombin and antithrombin. Sepharose 56-63 serpin family C member 1 Homo sapiens 126-138 7096332-2 1982 Unfractionated proteoglycan and proteoglycan fractions with low affinity and high affinity separated on antithrombin III-agarose were treated with alkali, releasing heparin chains (Mr congruent to 1 x 10(5)). Sepharose 121-128 serpin family C member 1 Rattus norvegicus 104-120 6215059-2 1982 The protein, SAIP (for serum actin inhibitory protein), has been purified by affinity chromatography of serum over actin-Sepharose followed by protein fractionation with ammonium sulfate and chromatography over DEAE-cellulose. Sepharose 121-130 actin Oryctolagus cuniculus 29-34 6215059-2 1982 The protein, SAIP (for serum actin inhibitory protein), has been purified by affinity chromatography of serum over actin-Sepharose followed by protein fractionation with ammonium sulfate and chromatography over DEAE-cellulose. Sepharose 121-130 actin Oryctolagus cuniculus 115-120 6982067-1 1982 The androgen receptor has been purified from steer seminal vesicle cytosol by a combination of differential DNA-Sepharose 4B chromatography and testosterone 17 beta-hemisuccinyl-3,3"-diaminodipropylamine-Sepharose 4B affinity chromatography. Sepharose 112-124 androgen receptor Homo sapiens 4-21 6982067-1 1982 The androgen receptor has been purified from steer seminal vesicle cytosol by a combination of differential DNA-Sepharose 4B chromatography and testosterone 17 beta-hemisuccinyl-3,3"-diaminodipropylamine-Sepharose 4B affinity chromatography. Sepharose 204-216 androgen receptor Homo sapiens 4-21 7201471-5 1982 The size of Endo A and Endo B messenger RNA has been determined by denaturing methyl mercury hydroxide agarose gels to be 2.0 +/- 0.1 and 1.5 +/- 0.2 kilobases, respectively. Sepharose 103-110 keratin 8 Mus musculus 12-18 7115764-8 1982 A fourth population, proteoglycan 3 (Kav=0.33, Sepharose 2B and CL-2B) was intracellular in osteoarthrotic cultures. Sepharose 47-56 proteoglycan 3, pro eosinophil major basic protein 2 Homo sapiens 21-35 7138503-2 1982 This activity co-purified with renin enzymic activity until separation by aminohexyl-Sepharose--pepstatin affinity chromatography. Sepharose 85-94 renin Homo sapiens 31-36 6749687-2 1982 Removal of the fibronectin by absorption of the plasma with agarose-immobilized gelatin abolished the attachment-promoting activity. Sepharose 60-67 fibronectin 1 Homo sapiens 15-26 7138504-3 1982 In affinity chromatography under physiological conditions, serum was depleted of fibronectin when run through columns of the carbodi-imide-treated proteins coupled to agarose. Sepharose 167-174 fibronectin 1 Homo sapiens 81-92 7119456-1 1982 Purification of radiolabeled carcinoembryonic antigen (CEA) preparations by affinity chromatography with anti-AG bound to Sepharose was attempted, since an immunological similarity between AG (alpha 1-acid glycoprotein) and a portion of CEA had been noted. Sepharose 122-131 CEA cell adhesion molecule 3 Homo sapiens 29-53 6288565-3 1982 In the present study, a fibronectin-binding protein was purified from sonicated S. aureus strain E2371 by affinity chromatography on fibronectin-Sepharose. Sepharose 145-154 fibronectin 1 Homo sapiens 24-35 6288565-3 1982 In the present study, a fibronectin-binding protein was purified from sonicated S. aureus strain E2371 by affinity chromatography on fibronectin-Sepharose. Sepharose 145-154 fibronectin 1 Homo sapiens 133-144 6288565-5 1982 The fibronectin-binding protein was released from fibronectin-Sepharose by carbamide (8 M). Sepharose 62-71 fibronectin 1 Homo sapiens 4-15 6288565-5 1982 The fibronectin-binding protein was released from fibronectin-Sepharose by carbamide (8 M). Sepharose 62-71 fibronectin 1 Homo sapiens 50-61 6979575-6 1982 The IgE-binding factors formed by both clones bound to rat IgE-coated Sepharose and could be eluted from the beads at pH 3.0. Sepharose 70-79 immunoglobulin heavy constant epsilon Homo sapiens 4-7 6979575-6 1982 The IgE-binding factors formed by both clones bound to rat IgE-coated Sepharose and could be eluted from the beads at pH 3.0. Sepharose 70-79 immunoglobulin heavy constant epsilon Homo sapiens 59-62 6980256-1 1982 Interleukin 2 (IL-2), produced with and without co-stimulation by the Burkitt"s lymphoma line Daudi, was purified 37,000-fold to apparent homogeneity from lymphocyte conditioned medium by (NH4)2SO4 precipitation, DEAE-cellulose ion-exchange chromatography, gel filtration, and chromatography on blue agarose and on Procion-red agarose. Sepharose 300-307 interleukin 2 Homo sapiens 0-13 6980256-1 1982 Interleukin 2 (IL-2), produced with and without co-stimulation by the Burkitt"s lymphoma line Daudi, was purified 37,000-fold to apparent homogeneity from lymphocyte conditioned medium by (NH4)2SO4 precipitation, DEAE-cellulose ion-exchange chromatography, gel filtration, and chromatography on blue agarose and on Procion-red agarose. Sepharose 327-334 interleukin 2 Homo sapiens 0-13 7119456-1 1982 Purification of radiolabeled carcinoembryonic antigen (CEA) preparations by affinity chromatography with anti-AG bound to Sepharose was attempted, since an immunological similarity between AG (alpha 1-acid glycoprotein) and a portion of CEA had been noted. Sepharose 122-131 CEA cell adhesion molecule 3 Homo sapiens 55-58 7119456-10 1982 Purification of CEA is possible by affinity chromatography with anti-AG bound to Sepharose. Sepharose 81-90 CEA cell adhesion molecule 3 Homo sapiens 16-19 6127240-2 1982 The specific antisera against somatostatin-28 were prepared from somatostatin-28 antisera by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 137-149 somatostatin Homo sapiens 30-45 6127240-2 1982 The specific antisera against somatostatin-28 were prepared from somatostatin-28 antisera by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 137-149 somatostatin Homo sapiens 150-165 7104346-2 1982 The vesicles adhere to an immunoadsorbent prepared by coupling antibodies raised against three of the principal proteins of the brush border membrane (aminopeptidase, sucrase-isomaltase and lactase) to Sepharose 4B. Sepharose 202-211 sucrase-isomaltase Sus scrofa 167-197 7104393-7 1982 The estrogen receptor interacted both with heparin and with DNA covalently coupled to agarose, and was eluted from either column by NaCl. Sepharose 86-93 estrogen receptor 1 Rattus norvegicus 4-21 7104393-8 1982 Chromatography of crude cytosol on heparin-agarose or DNA-agarose lead to an at least 10-fold or 25-fold purification of the estrogen receptor, respectively. Sepharose 43-50 estrogen receptor 1 Rattus norvegicus 125-142 7104393-8 1982 Chromatography of crude cytosol on heparin-agarose or DNA-agarose lead to an at least 10-fold or 25-fold purification of the estrogen receptor, respectively. Sepharose 58-65 estrogen receptor 1 Rattus norvegicus 125-142 7104741-1 1982 Glia maturation factor (GMF) immobilized on agarose beads retained the same mitogenic and morphological transforming activities as free GMF when tested on glioblasts. Sepharose 44-51 glia maturation factor beta Homo sapiens 0-22 7104741-1 1982 Glia maturation factor (GMF) immobilized on agarose beads retained the same mitogenic and morphological transforming activities as free GMF when tested on glioblasts. Sepharose 44-51 glia maturation factor beta Homo sapiens 24-27 6285697-2 1982 Alpha-galactosidase A was purified from human placenta by chromatography on concanavalin A-Sepharose, DEAE-cellulose, and N-epsilon-aminocaproyl-alpha-D-galactosylamine-Sepharose. Sepharose 91-100 galactosidase alpha Homo sapiens 0-21 6285697-2 1982 Alpha-galactosidase A was purified from human placenta by chromatography on concanavalin A-Sepharose, DEAE-cellulose, and N-epsilon-aminocaproyl-alpha-D-galactosylamine-Sepharose. Sepharose 169-178 galactosidase alpha Homo sapiens 0-21 24234283-2 1982 The solubilized enzyme was purified by affinity chromatography on hen ovomucoid-sepharose column.The purified galactosyltransferase showed three protein bands of approx. Sepharose 80-89 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 110-131 6289933-1 1982 Interaction between human neutrophils and fibronectin bound to gelatin-Sepharose granules was studied. Sepharose 71-80 fibronectin 1 Homo sapiens 42-53 6289933-2 1982 It was found that fibronectin following cell-induced dissociation from gelatin-Sepharose caused neutrophil aggregation which was inhibited by EDTA or pretrypsinization of the cells. Sepharose 79-88 fibronectin 1 Homo sapiens 18-29 6979358-2 1982 A quantity of 100 X 10(6) units of purified anti-CSF was coupled to cyanogen bromide activated Sepharose 4B; colony assays revealed complete binding of the antibodies to the gel. Sepharose 95-107 colony stimulating factor 2 Homo sapiens 49-52 7117245-2 1982 The proteins retained biological activity; they were purified further by affinity chromatography on calmodulin-conjugated CNBr-Sepharose 4B and used for antibody purification. Sepharose 127-139 calmodulin 1 Homo sapiens 100-110 6809059-1 1982 Ornithine decarboxylase (EC 4.1.1.17) was purified to near homogeneity from the livers of thioacetamide- and DL-alpha-hydrazino-delta aminovaleric acid-treated rats by using three types of affinity chromatography with pyridoxamine phosphate-Sepharose, pyridoxamine phosphate-dipropylenetriamine-Sepharose and heparin-Sepharose. Sepharose 241-250 ornithine decarboxylase 1 Rattus norvegicus 0-23 6809059-1 1982 Ornithine decarboxylase (EC 4.1.1.17) was purified to near homogeneity from the livers of thioacetamide- and DL-alpha-hydrazino-delta aminovaleric acid-treated rats by using three types of affinity chromatography with pyridoxamine phosphate-Sepharose, pyridoxamine phosphate-dipropylenetriamine-Sepharose and heparin-Sepharose. Sepharose 295-304 ornithine decarboxylase 1 Rattus norvegicus 0-23 6809059-1 1982 Ornithine decarboxylase (EC 4.1.1.17) was purified to near homogeneity from the livers of thioacetamide- and DL-alpha-hydrazino-delta aminovaleric acid-treated rats by using three types of affinity chromatography with pyridoxamine phosphate-Sepharose, pyridoxamine phosphate-dipropylenetriamine-Sepharose and heparin-Sepharose. Sepharose 295-304 ornithine decarboxylase 1 Rattus norvegicus 0-23 7096593-3 1982 Fibronectin isolated from pleural fluid by affinity chromatography on gelatin-Sepharose had a polypeptide pattern similar to that of plasma fibronectin in SDS-polyacrylamide gel electrophoresis. Sepharose 78-87 fibronectin 1 Homo sapiens 0-11 7096594-1 1982 During agarose electrophoresis C4 in the normal human serum is converted into cleavage products of Beta 1 and Beta 2 mobility. Sepharose 7-14 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 99-105 7096594-1 1982 During agarose electrophoresis C4 in the normal human serum is converted into cleavage products of Beta 1 and Beta 2 mobility. Sepharose 7-14 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 110-116 6291012-7 1982 On gel chromatography with Sepharose 6B column, the solubilized TRH-receptor molecule eluted at the fraction corresponding to an apparent molecular weight of 300,000 daltons, with Stokes" radius of 5.8 nm. Sepharose 27-36 thyrotropin releasing hormone Rattus norvegicus 64-67 7045574-2 1982 Solubilized, partially purified insulin receptor from human placenta binds to affinity resins in which N alpha,B1-biotinylinsulin is noncovalently attached to AH Sepharose 4B-immobilized-succinolylavidin. Sepharose 162-174 insulin receptor Homo sapiens 32-48 6956858-3 1982 Methylmercuric hydroxide/agarose gel analysis showed that human hypoxanthine/guanine phosphoribosyltransferase mRNA contains approximately 1,530 nucleotides, which is twice the number required for its protein coding capacity. Sepharose 25-32 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 64-110 6812233-1 1982 Simplified procedures have been developed for isolation of human plasma fibronectin by affinity chromatography on gelatin-agarose. Sepharose 122-129 fibronectin 1 Homo sapiens 72-83 6214865-0 1982 Fractionation of plasmic fibrinogen digest on lysine-agarose. Sepharose 53-60 fibrinogen beta chain Homo sapiens 25-35 6812233-3 1982 In a shorter process, fibronectin is eluted from gelatin-agarose simply by decreasing the buffer pH below 6. Sepharose 57-64 fibronectin 1 Homo sapiens 22-33 6921108-1 1982 Elongation factor Tu from Thermus thermophilus was coupled to cyanogen-bromide-activated Sepharose 4B and its properties were investigated. Sepharose 89-98 elongation factor Tu Thermus thermophilus HB8 0-20 7082587-7 1982 The two populations of AT III were separated by affinity chromatography on heparin-agarose. Sepharose 83-90 serpin family C member 1 Homo sapiens 23-29 6896820-9 1982 Myosin kinase II activity was completely Ca(2+)-dependent after affinity chromatography on the calmodulin-Sepharose column. Sepharose 106-115 calmodulin Bos taurus 95-105 6981412-1 1982 3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two triazine dye-Sepharose matrices. Sepharose 211-220 malic enzyme 1 Homo sapiens 50-70 6281387-1 1982 Acid sphingomyelinase (sphingomyelin phosphodiesterase, EC 3.1.4.12) was purified from human brain by extraction with 0.1% Triton X-100, followed by sequential chromatography on Concanavalin A-Sepharose, octyl-Sepharose, hydroxylapatite, DEAE-cellulose, red A-agarose, Sephadex G-200, and DEAE-cellulose with ampholyte elution. Sepharose 193-202 sphingomyelin phosphodiesterase 1 Homo sapiens 0-21 6281387-1 1982 Acid sphingomyelinase (sphingomyelin phosphodiesterase, EC 3.1.4.12) was purified from human brain by extraction with 0.1% Triton X-100, followed by sequential chromatography on Concanavalin A-Sepharose, octyl-Sepharose, hydroxylapatite, DEAE-cellulose, red A-agarose, Sephadex G-200, and DEAE-cellulose with ampholyte elution. Sepharose 193-202 sphingomyelin phosphodiesterase 1 Homo sapiens 23-54 6955933-2 1982 It was found that the main component was anionic and was eluted between albumin and lysozyme on Sepharose. Sepharose 96-105 albumin Homo sapiens 72-79 6121806-2 1982 gamma-Glutamylcysteine synthetase, previously known to be potently inhibited by cystamine, has been found to bind covalently to cystamine-Sepharose. Sepharose 138-147 glutamate-cysteine ligase catalytic subunit Homo sapiens 0-33 7201323-3 1982 Since the blood protein testosterone-estradiol binding globulin (TeBG) was a possible component of the epididymal extract, ABP was differentiated and separated from TeBG by affinity chromatography on Con A-Sepharose since the latter protein was shown to be completely absorbed by the lectin while the former was not. Sepharose 206-215 sex hormone-binding globulin Oryctolagus cuniculus 123-126 7126036-1 1982 Acetone powder extracts prepared from cultured pig aortic smooth muscle cells and the culture medium from these cells (particularly when incubated with heparin) were shown to contain a lipolytic enzyme which was identified as lipoprotein lipase by the following criteria: 1) stimulation by apolipoprotein C-II; 2) an optimal activity at approximately pH 8.0; 3) inhibition by NaCl, and 4) binding to a heparin-Sepharose affinity column. Sepharose 410-419 lipoprotein lipase Sus scrofa 226-244 7115810-2 1982 Active immobilized monomers of glyceraldehyde 3-phosphate dehydrogenase were prepared by means of dissociation of the tetrameric enzyme molecule covalently bound to Sepharose via a single subunit. Sepharose 165-174 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 31-71 6808514-5 1982 By using affinity chromatography with columns of anti-HPA52 IgG1 bound to Sepharose, HPA52 was purified from urokinase to homogeneity as evaluated by NaDodSO(4)/polyacrylamide gel electrophoresis. Sepharose 74-83 LOC105243590 Mus musculus 60-64 6124858-1 1982 Specific antisera against somatostatin-28 were prepared by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 103-115 somatostatin Homo sapiens 26-41 6124858-1 1982 Specific antisera against somatostatin-28 were prepared by absorption of somatostatin-28 antisera with sepharose 4B-somatostatin-14. Sepharose 103-115 somatostatin Homo sapiens 116-131 6178587-1 1982 The androgen receptor from mouse kidney cytosol binds not only to DNA but also to RNA as assayed by competition with DNA-cellulose centrifugation or by direct binding with agarose-polynucleotides. Sepharose 172-179 androgen receptor Mus musculus 4-21 7082587-8 1982 50% of the patients" AT III bound to the agarose beads. Sepharose 41-48 serpin family C member 1 Homo sapiens 21-27 6214252-5 1982 The antibody against HMG protein 17 in the IgG (immunoglobulin G) fraction of the ascites fluid was obtained by Protein A-Sepharose column chromatography. Sepharose 122-131 high mobility group nucleosomal binding domain 4 Gallus gallus 21-24 7044138-9 1982 3) alpha-Casein-Sepharose column also separated neutral proteases and immunoreactive renin from acid protease capable of generating angiotensin. Sepharose 16-25 renin Rattus norvegicus 85-90 6122608-6 1982 Preparation of calmodulin-free tryptophan hydroxylase by affinity chromatography on fluphenazine-Sepharose 4B yields an enzyme that is no longer activated by ATP-Mg2+, whereas the readdition of calmodulin to a calmodulin-free enzyme restores the responsiveness of tryptophan hydroxylase to ATP-Mg2+. Sepharose 97-106 calmodulin 1 Homo sapiens 15-25 24234228-5 1982 The slight differences observed when fumarase is immobilized at concentrations higher than 50 [Symbol: see text]g/g gel must be attributed to diffusional limitation at the surface of the Sepharose matrix. Sepharose 187-196 fumarate hydratase Sus scrofa 37-45 7152133-7 1982 The solubilized photolabeled insulin receptor fraction was enriched by first adsorbing to agarose-bound wheat germ agglutinin and the material eluted with N-acetyl-D-glucosamine was then analyzed by SDS-PAGE and autoradiography. Sepharose 90-97 insulin Homo sapiens 29-36 6122608-6 1982 Preparation of calmodulin-free tryptophan hydroxylase by affinity chromatography on fluphenazine-Sepharose 4B yields an enzyme that is no longer activated by ATP-Mg2+, whereas the readdition of calmodulin to a calmodulin-free enzyme restores the responsiveness of tryptophan hydroxylase to ATP-Mg2+. Sepharose 97-106 calmodulin 1 Homo sapiens 194-204 6122608-6 1982 Preparation of calmodulin-free tryptophan hydroxylase by affinity chromatography on fluphenazine-Sepharose 4B yields an enzyme that is no longer activated by ATP-Mg2+, whereas the readdition of calmodulin to a calmodulin-free enzyme restores the responsiveness of tryptophan hydroxylase to ATP-Mg2+. Sepharose 97-106 calmodulin 1 Homo sapiens 194-204 7040242-2 1982 In contrast to active renin, inactive renin from each source bound to Cibacron blue-agarose and was unable to bind to pepstatin-Sepharose. Sepharose 84-91 renin Homo sapiens 38-43 7040242-3 1982 Both plasma and renal inactive renin had weaker affinity for anion-exchange resins than the active form, both bound to concanavalin A-Sepharose and were eluted with carbohydrate, and both bound tightly to hydrophobic gels. Sepharose 134-143 renin Homo sapiens 31-36 6808694-0 1982 Chromatography of antihemophilic factor on diaminoalkane- and aminoalkane-derivatized Sepharose. Sepharose 86-95 coagulation factor VIII Homo sapiens 18-39 6461713-4 1982 All B cell- and C3b-specific activities of the aaH were removed and subsequently recovered by absorption and elution of the antibody from either C3-agarose or goat-anti-H-agarose. Sepharose 148-155 aspartate beta-hydroxylase Homo sapiens 47-50 6285179-1 1982 DNase treatment used to convert supercoiled DNAs to the open form is suitable for molecular weight determination by electron microscopy or agarose gel electrophoresis. Sepharose 139-146 colicin E8 Escherichia coli 0-5 7048734-2 1982 Efficiency of the oxidation was monitored by a decrease in specific binding of alpha-glucosidase with concanavaline A-Sepharose. Sepharose 118-127 sucrase-isomaltase Homo sapiens 79-96 6808694-1 1982 Antihemophilic factor was chromatographed on a homologous series of diaminoalkane- and aminoalkane-modified Sepharose beads. Sepharose 108-117 coagulation factor VIII Homo sapiens 0-21 6280480-3 1982 The elution profile of angiotensin I-converting enzyme on DEAE cellulose and after gel filtration on Sepharose 4B was unchanged by captopril. Sepharose 101-113 angiotensin I converting enzyme Rattus norvegicus 23-54 6174524-5 1982 Both antibodies eluted from the Protein A-Sepharose as a single subclass, IgG1, and directly inhibited choline acetyltransferase activity. Sepharose 42-51 Choline acetyltransferase Drosophila melanogaster 103-128 6802184-1 1982 Lipoprotein lipase was purified from guinea pig milk by chromatography on heparin-Sepharose followed by chromatography on an immobilized preparation of heparin that had been N-desulphated and then acetylated. Sepharose 82-91 lipoprotein lipase Cavia porcellus 0-18 7112509-0 1982 Preparation of highly stable antithrombin-sepharose and utilization for the fractionation of heparin. Sepharose 42-51 serpin family C member 1 Homo sapiens 29-41 7047272-2 1982 Insulin specific IgE and IgG are bound to insulin covalently coupled to Sepharose particles. Sepharose 72-81 insulin Homo sapiens 0-7 6802850-2 1982 Five forms of cytochrome P-450 have been purified from liver microsomes of beta-naphthoflavone-pretreated rats by chromatography on DEAE-Sephadex, DEAE-cellulose, and hydroxylapatite or CM-Sepharose columns. Sepharose 189-198 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 14-30 6277954-1 1982 A 93,000 molecular weight protein (HBP.93) which binds hemin and protoporphyrin IX with high affinity has been isolated from rabbit serum using affinity chromatography on hemin-conjugated agarose. Sepharose 188-195 heme binding protein 1 Homo sapiens 35-38 7061483-3 1982 Fractionation of supernatant solutions of normal goat liver on columns of concanavalin A bound to Sepharose 4B resolved beta-mannosidase into a bound (acidic) form (pH optimum, 5.0 to 5.5) and an unbound (neutral) form (broad pH optimum from 5.0 to 8.0). Sepharose 98-107 beta-mannosidase Capra hircus 120-136 7060530-6 1982 For [3H]tamoxifen and [3H]5 alpha-androstanediol, ternary complexes were also formed with ER bound to monoclonal antibodies linked to Sepharose. Sepharose 134-143 estrogen receptor 1 Bos taurus 90-92 7042918-0 1982 Agarose isoelectric focusing of unconcentrated CSF and radioimmunofixation for detection of oligoclonal bands in patients with multiple sclerosis and other neurological diseases. Sepharose 0-7 colony stimulating factor 2 Homo sapiens 47-50 6977565-13 1982 Monoclonal antibodies coupled to Sepharose 4B absorbed IL 2 crude culture supernatant, confirming that they react directly with IL 2. Sepharose 33-45 interleukin 2 Homo sapiens 55-59 7082636-9 1982 The deduction from the above of ligand-induced dimerization is evident also in the increased retardation of NPII on neurophysin--Sepharose when the eluting buffer contains soluble peptide hormone. Sepharose 129-138 arginine vasopressin Bos taurus 108-112 6977565-13 1982 Monoclonal antibodies coupled to Sepharose 4B absorbed IL 2 crude culture supernatant, confirming that they react directly with IL 2. Sepharose 33-45 interleukin 2 Homo sapiens 128-132 7069515-6 1982 The mRNA was translated in a wheat germ system and newly synthesized metallothionein was isolated by activated thiol--Sepharose 4B chromatography. Sepharose 118-127 metallothionein-like protein 1 Triticum aestivum 69-84 6895893-3 1982 The inhibitor, designated heparin cofactor II (HCII), was purified to homogeneity with sulfated-dextran, DEAE-Sepharose, heparin-Sepharose and Sephadex G-150. Sepharose 110-119 serpin family D member 1 Homo sapiens 26-45 6895893-3 1982 The inhibitor, designated heparin cofactor II (HCII), was purified to homogeneity with sulfated-dextran, DEAE-Sepharose, heparin-Sepharose and Sephadex G-150. Sepharose 110-119 serpin family D member 1 Homo sapiens 47-51 7058344-1 1982 An enzyme purified from squid nerve that hydrolyzes the cholinesterase inhibitor diisopropyl phosphorofluoridate (DFP) has now been coupled to agarose beads. Sepharose 143-150 butyrylcholinesterase Homo sapiens 56-70 6284125-2 1982 A fibronectin-binding protein as partially purified from washed solubilized human platelet membranes by affinity chromatography on fibronectin-Sepharose. Sepharose 143-152 fibronectin 1 Homo sapiens 2-13 6284125-2 1982 A fibronectin-binding protein as partially purified from washed solubilized human platelet membranes by affinity chromatography on fibronectin-Sepharose. Sepharose 143-152 fibronectin 1 Homo sapiens 131-142 6177002-7 1982 Native IFN-gamma was found to have a lower affinity for alkyl agarose columns than human IFN-alpha or IFN-beta did, suggesting that IFN-gamma is a relatively hydrophilic protein. Sepharose 62-69 interferon gamma Homo sapiens 7-16 7126333-1 1982 By exploiting the cross-reaction among the antigens associated with a pool of breast carcinomas, preselected according to their antigenic content, a CEA-like BCA, with a specific activity of 1400 U/micrograms has been extracted, by means of 3M KCl, and partially purified by means of ConA Sepharose affinity chromatography and bioabsorption, employing, for the latter, a cross-reacting antiserum (anti CEA). Sepharose 289-298 CEA cell adhesion molecule 3 Homo sapiens 149-152 6281363-3 1982 The N. crassa actin was purified by DEAE-cellulose and DNAase I-Sepharose chromatography and had the expected property of inhibiting DNAase I activity. Sepharose 64-73 actin Oryctolagus cuniculus 14-19 7085752-6 1982 The ciliary protein is identified as a calmodulin on the basis of its calcium- dependent binding to a fluphenazine-sepharose affinity column and its comigration with bovine brain calmodulin on alkaline-urea and SDS polyacrylamide gels in both the presence and absence of calcium. Sepharose 115-124 calmodulin Bos taurus 39-49 6177002-7 1982 Native IFN-gamma was found to have a lower affinity for alkyl agarose columns than human IFN-alpha or IFN-beta did, suggesting that IFN-gamma is a relatively hydrophilic protein. Sepharose 62-69 interferon gamma Homo sapiens 132-141 6461976-11 1982 After gel filtration through an Ultrogel or Sepharose column containing 1% cholate in the elution buffer the delipidated ATPase is eluted as a reactivatable high molecular aggregate, whereas 1% deoxycholate favours the formation of completely lipid-free monomeric units which cannot be reactivated, however. Sepharose 44-53 dynein axonemal heavy chain 8 Homo sapiens 121-127 6951202-4 1982 The enzyme was characterized as lipoprotein lipase (triacylglycero-protein acylhydrolase, EC 3.1.1.34) on the basis of pH optimum (7.8--8.2 for monocytes, 8.1 for alveolar macrophages), dependence on apolipoprotein C-II for activity, inhibition by 0.3--0.5 M sodium chloride and protamine sulfate, and retention of a heparin-Sepharose gel. Sepharose 325-334 lipoprotein lipase Homo sapiens 32-50 7066431-0 1982 [Affinity chromatography of menadione reductase on Sepharose with aminoaromatic and aminoaliphatic quinone ligands]. Sepharose 51-60 NAD(P)H quinone dehydrogenase 1 Homo sapiens 28-47 6917718-5 1982 The mediums were collected and assayed for elastolytic activity using Sepharose-coupled [3H] soluble elastin as substrate. Sepharose 70-79 elastin Homo sapiens 101-108 6175043-3 1982 Compared to the similar antithrombin III, this glycoprotein binds only moderately to porcine heparin (hence its name Antithrombin BM), thus requiring higher heparin concentration for full thrombin inhibitor function, and lower ionic strength for elution from a heparin sepharose column. Sepharose 269-278 serpin family C member 1 Homo sapiens 117-129 7060565-8 1982 Kinetic constants measured for the release of angiotensin I by renin were found to be Km = 5.0 microM proangiotensin and V = 270 nmol of angiotensin I h-1 unit renin-1 for the concanavalin-A-binding form and Km = 5.6 microM proangiotensin and V = 250 nmol angiotensin I h-1 unit renin-1 for the prohormone which did not bind to concanavalin-A--Sepharose. Sepharose 344-353 renin Rattus norvegicus 63-68 7068586-1 1982 The interaction of porcine plasma fibronectin and its proteolytic fragments with hyaluronic acid was investigated by affinity chromatography using hyaluronic acid-linked Sepharose 4B. Sepharose 170-179 fibronectin 1 Homo sapiens 34-45 6175959-3 1982 Fragment C3d remained attached to the thiol-Sepharose and was subsequently eluted with L-cysteine. Sepharose 44-53 endogenous retrovirus group K member 13 Homo sapiens 9-12 6275890-1 1982 Use of thiol-sepharose-bound Saccharomyces cerevisiae cytochrome c. A method for simultaneous purification of cytochrome c reductase and cytochrome c oxidase using a cytochrome c affinity column is presented. Sepharose 13-22 cytochrome c oxidase subunit 6A1, mitochondrial Bos taurus 137-157 6119171-2 1982 Chromatography of the P105 fraction on Sepharose 4B and 2B revealed that gamma-glutamyltranspeptidase and aminopeptidase activities had a molecular mass of 20 X 10(5) to 40 X 10(6), whereas in the S105 fraction soluble gamma-glutamyltranspeptidase and aminopeptidase had 86000 and 160000, respectively. Sepharose 39-48 carboxypeptidase Q Homo sapiens 106-120 7136937-5 1982 Human serum depleted of opsonic fibronectin by gelatin-sepharose affinity chromatography manifested a marked reduction in its ability to support phagocytosis of S aureus by human neutrophils. Sepharose 55-64 fibronectin 1 Homo sapiens 32-43 6807178-5 1982 Precipitation of purified SAP by calcium could be prevented by pretreatment with acid hydrolysates of agarose or SP Sephadex, matrices for which SAP has a calcium-dependent affinity. Sepharose 102-109 amyloid P component, serum Homo sapiens 26-29 6807178-5 1982 Precipitation of purified SAP by calcium could be prevented by pretreatment with acid hydrolysates of agarose or SP Sephadex, matrices for which SAP has a calcium-dependent affinity. Sepharose 102-109 amyloid P component, serum Homo sapiens 145-148 6807178-7 1982 While the identity of natural ligand for SAP is as yet unknown, it is likely to resemble the glycosidic subunits in agarose. Sepharose 116-123 amyloid P component, serum Homo sapiens 41-44 6805462-6 1982 Spermidine oxidase activity and diamine oxidase activity eluted in a concerted fashion when pregnancy serum was subjected to cadaverine-Sepharose chromatography, gel filtration and ion-exchange chromatography. Sepharose 136-145 amine oxidase copper containing 1 Homo sapiens 32-47 6275893-2 1982 Proenzymic C1r was purified from human plasma in a two-step technique involving indirect affinity chromatography on Sepharose Ig anti-C1s. Sepharose 116-125 complement C1r Homo sapiens 11-14 7049440-2 1982 Renin was separated from cathepsin by affinity chromatography on casein-Sepharose. Sepharose 72-81 renin Homo sapiens 0-5 7082278-1 1982 The binding of fibronectin to gelatin-agarose was strictly dependent on pH, having a pH optimum of 7-9. Sepharose 38-45 fibronectin 1 Homo sapiens 15-26 7075717-4 1982 When 60 ng CEA were preincubated with highly diluted anti-CEA serum and the resulting immune complexes were thereafter co-precipitated by protein A-sepharose, positive lymphocyte responses could no longer be obtained. Sepharose 148-157 CEA cell adhesion molecule 3 Homo sapiens 11-14 6129971-2 1982 Using agarose gel electrophoresis, a faster moving alanine aminopeptidase (EC 3.4.11.2) has been demonstrated in the urine from cases of Fanconi syndrome, endemic (Balkan) nephropathy and advanced renal insufficiency. Sepharose 6-13 carboxypeptidase Q Homo sapiens 59-73 6182004-3 1982 The inhibition was abolished by removal of urinary alpha1-antitrypsin by trypsin-sepharose treatment. Sepharose 81-90 serpin family A member 1 Homo sapiens 51-69 6757202-2 1982 The rabbit antihuman fibronectin was purified by affinity chromatography on human fibronectin-Sepharose. Sepharose 94-103 fibronectin 1 Homo sapiens 21-32 6757202-2 1982 The rabbit antihuman fibronectin was purified by affinity chromatography on human fibronectin-Sepharose. Sepharose 94-103 fibronectin 1 Homo sapiens 82-93 6178683-2 1982 Acrosin was purified by molecular sieve and proflavin-Sepharose affinity chromatography. Sepharose 54-63 acrosin Oryctolagus cuniculus 0-7 6212529-1 1982 A total of 1,086 individuals from Galicia have been typed for red cell PGM1 using isoelectric focusing in polyacrylamide and agarose gels. Sepharose 125-132 phosphoglucomutase 1 Homo sapiens 71-75 7095815-1 1982 Three new phenotypes of plasminogen system, named PLG 3-1, PLG 1-M and PLG 1-C, were found in sera from healthy Japanese persons by the agarose gel isoelectric focusing followed by the methods of immunofixation and caseinolysis. Sepharose 136-143 plasminogen Homo sapiens 50-53 7173979-1 1982 Human serum IgA was isolated employing a system of G-200 column chromatography, anion exchange (DE-52) chromatography, and passage over protein A and anti-IgG Sepharose 4B columns. Sepharose 159-168 CD79a molecule Homo sapiens 12-15 6176562-2 1982 AFP is purified by anion exchange chromatography on DEAE-Sephadex A 50, affinity chromatography on Con-A-Sepharose, and preparative electrophoresis on agarose gel. Sepharose 105-114 alpha fetoprotein Homo sapiens 0-3 6176562-2 1982 AFP is purified by anion exchange chromatography on DEAE-Sephadex A 50, affinity chromatography on Con-A-Sepharose, and preparative electrophoresis on agarose gel. Sepharose 151-158 alpha fetoprotein Homo sapiens 0-3 6279583-1 1982 Sepharose 4B conjugated with phosphorylated myosin light chains was used in affinity chromatography of a partially purified preparation of gizzard myosin light-chain phosphatase (MLCP) (Onishi et al. Sepharose 0-12 myosin heavy chain 14 Homo sapiens 44-50 6279583-1 1982 Sepharose 4B conjugated with phosphorylated myosin light chains was used in affinity chromatography of a partially purified preparation of gizzard myosin light-chain phosphatase (MLCP) (Onishi et al. Sepharose 0-12 myosin heavy chain 14 Homo sapiens 147-153 7086148-6 1982 SAP was isolated from normal serum by calcium-dependent affinity chromatography on unsubstituted Sepharose beads, followed by solid-phase immunoabsorption of contaminants and finally gel filtration on Sephacryl S-300. Sepharose 97-106 amyloid P component, serum Homo sapiens 0-3 7142970-3 1982 However, when the disrupted virus particles and p30 were coupled to Sepharose in the presence of Triton X-100, stronger binding of the four proteins was observed. Sepharose 68-77 high mobility group box 1 Mus musculus 48-51 6182255-3 1982 The chromatographic behavior of human cornea IFN on Con A-Sepharose and zinc chelate agarose columns was identical to that of HuIFN-beta produced by human foreskin cells. Sepharose 58-67 interferon alpha 1 Homo sapiens 45-48 6811671-1 1982 Murine spleen lymphoid cells treated with insoluble (Sepharose bound) concanavalin A elaborate Gamma (immune) interferon (IFN-gamma) and macrophage activation factor (MAF) into the culture medium. Sepharose 53-62 interferon gamma Mus musculus 122-131 6811671-1 1982 Murine spleen lymphoid cells treated with insoluble (Sepharose bound) concanavalin A elaborate Gamma (immune) interferon (IFN-gamma) and macrophage activation factor (MAF) into the culture medium. Sepharose 53-62 avian musculoaponeurotic fibrosarcoma oncogene homolog Mus musculus 167-170 7109454-3 1982 The determination of activity of the second isoenzyme CPK-MV was carried out by electrophoretic method modified by the authors on agarose gel using Soviet appliances and the maintenance medium. Sepharose 130-137 phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha Homo sapiens 54-57 6212737-0 1982 Purification of rhodopsin on Agarose. Sepharose 29-36 rhodopsin Homo sapiens 16-25 20487909-1 1982 The breakdown of cytoplasmic tubulin from brain (purified by ammonium sulfate fractionation and DEAE cellulose chromatography) by cathepsin D from brain (purified by ammonium sulfate fractionation and pepstatin Sepharose chromatography) was studied; changes in the intensity of tubulin gel bands were determined. Sepharose 211-220 cathepsin D Homo sapiens 130-141 7088732-1 1982 Starting with human prostatic acid phosphatase (HPAP) purified by affinity chromatography on Sepharose-bound N-(6-aminohexyl)tartramic acid [Van Etten, R. L. and Saini, M. S. (1978) Clin. Sepharose 93-102 acid phosphatase 3 Homo sapiens 20-46 6980436-2 1982 AAT genetic phenotypes were determined using acid agarose gel electrophoresis, followed by crossed antigen-antibody electrophoresis in agarose gel. Sepharose 50-57 serpin family A member 1 Homo sapiens 0-3 6978519-1 1982 A nuclear SS-B antigen was isolated from a saline extract of acetone powder of rabbit thymus by precipitation with ammonium sulphate, affinity chromatography with Blue Sepharose CL-6B, and preparative agarose gel electrophoresis. Sepharose 201-208 lupus La protein homolog Oryctolagus cuniculus 10-14 7198118-3 1981 Molecular properties of the two lectins, measured as relative molecular weights, isoelectric and electrophoretic patterns, amino acid compositions, immunochemical cross-reactivity, and chromatographic behavior on Sepharose-concanavalin A adsorbents suggest that the seed and the root lectin are very similar but not identical. Sepharose 213-222 LOW QUALITY PROTEIN: lectin Glycine max 32-38 7298669-0 1981 Calcium-dependent affinity chromatography of S-100 and calmodulin on calmodulin antagonist-coupled Sepharose. Sepharose 99-108 calmodulin 1 Homo sapiens 55-65 7298669-0 1981 Calcium-dependent affinity chromatography of S-100 and calmodulin on calmodulin antagonist-coupled Sepharose. Sepharose 99-108 calmodulin 1 Homo sapiens 69-79 7317404-5 1981 When P450scc was incorporated into the dioleoylglycerophosphocholine liposomes by the cholate-filtration method, the P450scc-containing liposomes showed two major populations on the elution pattern of the Sepharose 4B gel filtration, and were seen at a diameter of 200-600 A and its aggregated forms. Sepharose 205-217 cholesterol side-chain cleavage enzyme, mitochondrial Bos taurus 5-12 7317404-5 1981 When P450scc was incorporated into the dioleoylglycerophosphocholine liposomes by the cholate-filtration method, the P450scc-containing liposomes showed two major populations on the elution pattern of the Sepharose 4B gel filtration, and were seen at a diameter of 200-600 A and its aggregated forms. Sepharose 205-217 cholesterol side-chain cleavage enzyme, mitochondrial Bos taurus 117-124 7035166-5 1981 More conclusive evidence for the non-association of the liver aryl acylamidase and cholinesterase came from their clear-cut separation on procainamide-Sepharose affinity chromatography. Sepharose 151-160 butyrylcholinesterase Homo sapiens 83-97 6794677-2 1981 A suspension of living lymphocytes is mixed with agarose sol containing the reagents for the detection of PNP or ADA activity on a glass slide. Sepharose 49-56 adenosine deaminase Homo sapiens 113-116 7337974-1 1981 Staphylococcal protein A (SPA), bound to CNBr-activated Sepharose, was evaluated as a selective adsorbent for soluble immune complexes (ICs) prepared in antigen (Ag) excess. Sepharose 56-65 pulmonary surfactant-associated protein A Oryctolagus cuniculus 26-29 7337974-8 1981 Recovery of ICs by SPA affinity chromatography was significantly decreased when the ICs were partially purified by PEG or ammonium sulphate precipitation before application to the SPA-Sepharose columns. Sepharose 184-193 pulmonary surfactant-associated protein A Oryctolagus cuniculus 180-183 6920385-2 1982 Chicken antithrombin was purified from fresh chicken plasma by affinity chromatography using heparin-agarose, and its amino acid and carbohydrate compositions, amino-terminal sequence, inhibition of human thrombin, and immunological properties were studied and compared with previously studied mammalian antithrombins (human, pig, rabbit, and rat), and also with chick ovalbumin. Sepharose 101-108 serpin family C member 1 Gallus gallus 8-20 7118585-8 1982 MB and PMS yielded almost equally high activities in the demonstration of the flavoprotein-dependent SDH using a reaction medium with agarose. Sepharose 134-141 succinate dehydrogenase complex iron sulfur subunit B Homo sapiens 101-104 7118585-10 1982 MPMS always had the lowest efficiency in electron transfer ability using an aqueous or agarose containing reaction medium (SDH). Sepharose 87-94 succinate dehydrogenase complex iron sulfur subunit B Homo sapiens 123-126 6917825-4 1982 A new GBG phenotype, supposedly heterozygous, with a slow migrating F band and a regular S band in agarose electrophoresis was observed. Sepharose 99-106 complement factor B Homo sapiens 6-9 6918356-2 1982 Polymorphism of the properdin factor B (BF) was investigated using an agarose gel immunofixation electrophoresis in 487 unrelated healthy adult Japanese who were already typed for HLA-A, -B, -C and C2. Sepharose 70-77 complement factor B Homo sapiens 20-43 7056569-1 1982 C3b receptor protein was purified form human erythrocytes by 2 M KBr solubilization and affinity chromatography on C3-coated sepharose. Sepharose 125-134 endogenous retrovirus group K member 3 Homo sapiens 0-3 6978269-2 1982 Equine alpha 1-antitrypsin was isolated from horse plasma by a combination of ammonium sulfate and acidification precipitation followed by ion-exchange chromatography on DEAE-cellulose, molecular sieve chromatography on Sephadex G-200 and affinity chromatography on Cibacron Blue-agarose. Sepharose 280-287 alpha-1-antiproteinase 2 Equus caballus 7-26 7037973-1 1982 By taking advantage of the ability of L-929 tumor cells to grow in agarose, we have developed an in vitro method for detecting tumor necrosis factor (TNF), a potent inhibitor of tumor cell growth. Sepharose 67-74 tumor necrosis factor Mus musculus 127-148 7037973-1 1982 By taking advantage of the ability of L-929 tumor cells to grow in agarose, we have developed an in vitro method for detecting tumor necrosis factor (TNF), a potent inhibitor of tumor cell growth. Sepharose 67-74 tumor necrosis factor Mus musculus 150-153 7037973-2 1982 When placed in a well cut in agarose containing L-cells, TNF inhibits L-cell growth in the area adjacent to the well. Sepharose 29-36 tumor necrosis factor Mus musculus 57-60 7086148-2 1982 CRP was obtained from malignant ascitic and pleural fluids by calcium-dependent affinity chromatography on pneumococcal C-polysaccharide covalently coupled to cyanogen bromide-activated Sepharose. Sepharose 186-195 C-reactive protein Homo sapiens 0-3 7086148-3 1982 It was then gel filtered on Ultrogel AcA44 (acrylamide-agarose beads) in the presence of calcium ions, combining molecular sieve chromatography with removal of contaminating SAP by its affinity of agarose. Sepharose 197-204 amyloid P component, serum Homo sapiens 174-177 6274414-8 1981 With this method we found von Willebrand factor receptors in the whole platelet extract, the Triton X-100 platelet membrane extract and in platelet extract material which bound to a bovine Willebrand factor-Sepharose 6MB affinity column. Sepharose 207-216 von Willebrand factor Bos taurus 26-47 7337233-0 1981 Fractionation of heparin using antithrombin III reversibly bound to concanavalin A-sepharose. Sepharose 83-92 serpin family C member 1 Homo sapiens 31-47 6273126-4 1981 2) Covalent immobilization of D-Lys6-des-Gly10-Pro9-ethylamide GnRH (a GnRH agonist) on agarose beads resulted in a derivative which stimulated LH release with full efficacy. Sepharose 88-95 gonadotropin releasing hormone 1 Homo sapiens 63-67 6273126-4 1981 2) Covalent immobilization of D-Lys6-des-Gly10-Pro9-ethylamide GnRH (a GnRH agonist) on agarose beads resulted in a derivative which stimulated LH release with full efficacy. Sepharose 88-95 gonadotropin releasing hormone 1 Homo sapiens 71-75 6916776-1 1981 Properdin factor B is a genetically-controlled polymorphism that can be demonstrated by agarose gel electrophoresis. Sepharose 88-95 complement factor B Homo sapiens 0-18 7330549-0 1981 [Valve of a freeze-dried gelatin-agarose combination in selective extraction of fibronectin from human plasma]. Sepharose 33-40 fibronectin 1 Homo sapiens 80-91 7330549-1 1981 We report here a simple procedure for the isolation of human plasma fibronectin by affinity adsorption on a new lyophilized adsorbent which is obtained by mixing and polymerizing agarose with gelatin. Sepharose 179-186 fibronectin 1 Homo sapiens 68-79 7038987-1 1981 Fibronectin was isolated from human plasma by affinity chromatography with gelatin-coupled Sepharose 4B. Sepharose 91-100 fibronectin 1 Homo sapiens 0-11 6795195-3 1981 The purified mucin gave a single band upon electrophoresis in either 5% acrylamide or 1% agarose gels. Sepharose 89-96 LOC100508689 Homo sapiens 13-18 6800672-0 1981 Practical requirements for immunochemical analysis of antithrombin III in agarose gels. Sepharose 74-81 serpin family C member 1 Homo sapiens 54-70 6800672-1 1981 This report emphasizes the importance charged groups affixed to the agarose gel matrix have on immunoprecipitation of antithrombin III (AT) and of its protease complexes. Sepharose 68-75 serpin family C member 1 Homo sapiens 118-134 7199000-2 1981 Bovine serum TeBG was purified by 17 alpha -carboxyethynyl-17-hydroxy-4-androsten-3-one sepharose 4B affinity chromatography (T-sepharose) and hydroxylapatite column chromatography. Sepharose 88-97 sex hormone binding globulin Homo sapiens 13-17 7199000-2 1981 Bovine serum TeBG was purified by 17 alpha -carboxyethynyl-17-hydroxy-4-androsten-3-one sepharose 4B affinity chromatography (T-sepharose) and hydroxylapatite column chromatography. Sepharose 128-137 sex hormone binding globulin Homo sapiens 13-17 6170112-1 1981 Translationally active (in Xenopus oocytes) human alpha interferon (IFN) messenger RNA"s (mRNA"s) derived from Sendai virus--induced leukocyte cultures display a bimodal distribution of RNA lengths on electrophoresis through agarose-CH3HgOH gels. Sepharose 225-232 interferon alpha 1 Homo sapiens 50-72 7340806-0 1981 A comparative study of the binding of cartilage link protein and the hyaluronate-binding region of the cartilage proteoglycan to hyaluronate-substituted Sepharose gel. Sepharose 153-162 hyaluronan and proteoglycan link protein 1 Bos taurus 38-60 7296000-1 1981 Two fractions of human prothrombin can be isolated from single donor plasma by the technique of heparin-agarose chromatography in (sodium) citrate buffer, pH 7.5, as previously reported for pooled plasma. Sepharose 104-111 coagulation factor II, thrombin Homo sapiens 23-34 7075717-4 1982 When 60 ng CEA were preincubated with highly diluted anti-CEA serum and the resulting immune complexes were thereafter co-precipitated by protein A-sepharose, positive lymphocyte responses could no longer be obtained. Sepharose 148-157 CEA cell adhesion molecule 3 Homo sapiens 58-61 6169737-4 1981 A single batch of antibody-bound Sepharose was used repeatedly to adsorb hCG beta from liter quantities of spent culture fluid containing 10% fetal bovine serum. Sepharose 33-42 chorionic gonadotropin subunit beta 3 Homo sapiens 73-81 6270179-8 1981 The small molecular size immunoreactive hCG-like substance bound to Concanavalin A-Sepharose 4B and eluted with 0.2 M alpha-D-methyl glucopyranoside, contained no significant intrinsic biological activity when tested in the in vitro Leydig cell bioassay and also failed to compete with labeled hCG for specific ovarian LH/hCG receptors. Sepharose 83-92 chorionic gonadotropin subunit beta 5 Homo sapiens 40-43 7338523-1 1981 Porcine intestinal heparin was partially digested with a purified heparinase and an octasaccharide with high-affinity for antithrombin III was isolated from the digest by gel filtration, followed by affinity chromatography on a column of Sepharose 4B coupled with antithrombin III. Sepharose 238-250 serpin family C member 1 Homo sapiens 122-138 7299240-0 1981 Stimulation of erythroid colony formation in vitro by erythropoietin immobilized on agarose-bound lectins. Sepharose 84-91 erythropoietin Homo sapiens 54-68 6947252-1 1981 We have purified the brain-specific protein S100b by affinity-based adsorption chromatography on phenothiazine-Sepharose conjugates and studied the interaction of this and other calcium-modulated proteins with the immobilized antipsychotic drug. Sepharose 111-120 S100 calcium binding protein B Bos taurus 44-49 6947252-2 1981 Bovine brain calmodulin, rabbit skeletal muscle troponin C, and bovine brain S100b bind to phenothiazine-Sepharose in a calcium-dependent manner. Sepharose 105-114 calmodulin Bos taurus 13-58 6947252-2 1981 Bovine brain calmodulin, rabbit skeletal muscle troponin C, and bovine brain S100b bind to phenothiazine-Sepharose in a calcium-dependent manner. Sepharose 105-114 S100 calcium binding protein B Bos taurus 77-82 7336661-1 1981 A procedure is described for isolation of prethrombin I using biospecific chromatography of the thrombin hydrolysate of prothrombin complex on heparin-Sepharose. Sepharose 151-160 prothrombin Oryctolagus cuniculus 45-53 7336661-1 1981 A procedure is described for isolation of prethrombin I using biospecific chromatography of the thrombin hydrolysate of prothrombin complex on heparin-Sepharose. Sepharose 151-160 prothrombin Oryctolagus cuniculus 120-131 6793584-3 1981 Antibodies raised against purified Protein I have been isolated from rabbit antiserum by affinity chromatography on Protein I-conjugated agarose column. Sepharose 137-144 annexin A2 Homo sapiens 35-44 6793584-3 1981 Antibodies raised against purified Protein I have been isolated from rabbit antiserum by affinity chromatography on Protein I-conjugated agarose column. Sepharose 137-144 annexin A2 Homo sapiens 116-125 6268632-3 1981 Gel filtration on acrylamide agarose (AcA-22) at 23 degrees C in the absence of transferrin indicates the transferrin receptor has a Stokes radius of 4.6 nm. Sepharose 29-36 small nucleolar RNA, H/ACA box 22 Homo sapiens 38-44 6268632-3 1981 Gel filtration on acrylamide agarose (AcA-22) at 23 degrees C in the absence of transferrin indicates the transferrin receptor has a Stokes radius of 4.6 nm. Sepharose 29-36 transferrin Homo sapiens 106-117 7318173-6 1981 (3) When plasma was subjected to gel filtration on columns of 6% agarose, apolipoprotein E was located in three regions. Sepharose 65-72 apolipoprotein E Homo sapiens 74-90 6274149-1 1981 Under specified conditions purified C1q, activated C1r and C1s and C1r-C1s complexes were bound independently of Ca2+, to heparin-Sepharose, and could be eluted by an increasing salt gradient. Sepharose 130-139 complement C1q A chain Homo sapiens 36-39 6274149-1 1981 Under specified conditions purified C1q, activated C1r and C1s and C1r-C1s complexes were bound independently of Ca2+, to heparin-Sepharose, and could be eluted by an increasing salt gradient. Sepharose 130-139 complement C1r Homo sapiens 51-54 6274149-1 1981 Under specified conditions purified C1q, activated C1r and C1s and C1r-C1s complexes were bound independently of Ca2+, to heparin-Sepharose, and could be eluted by an increasing salt gradient. Sepharose 130-139 complement C1s Homo sapiens 59-62 6274149-1 1981 Under specified conditions purified C1q, activated C1r and C1s and C1r-C1s complexes were bound independently of Ca2+, to heparin-Sepharose, and could be eluted by an increasing salt gradient. Sepharose 130-139 complement C1r Homo sapiens 67-70 6274149-1 1981 Under specified conditions purified C1q, activated C1r and C1s and C1r-C1s complexes were bound independently of Ca2+, to heparin-Sepharose, and could be eluted by an increasing salt gradient. Sepharose 130-139 complement C1s Homo sapiens 71-74 6274149-4 1981 In the presence of C1t (serum amyloid P component), C1s was firmly retained on heparin-Sepharose, which was probably due to formation of a C1s-C1t complex. Sepharose 87-96 complement C1s Homo sapiens 52-55 6274149-4 1981 In the presence of C1t (serum amyloid P component), C1s was firmly retained on heparin-Sepharose, which was probably due to formation of a C1s-C1t complex. Sepharose 87-96 complement C1s Homo sapiens 139-142 7030111-3 1981 In this investigation serum transferrin from healthy controls and alcoholic patients was purified by affinity chromatography on antitransferrin Sepharose 4B. Sepharose 144-156 transferrin Homo sapiens 28-39 6793685-5 1981 SAP, which had been aggregated either by direct conjugation to CNBr-activated Sepharose beads, or by complexing with anti-SAP antibodies immobilized on such beads, selectively took up fibronectin and C4-binding protein from whole normal human serum. Sepharose 78-87 amyloid P component, serum Homo sapiens 0-3 6793685-5 1981 SAP, which had been aggregated either by direct conjugation to CNBr-activated Sepharose beads, or by complexing with anti-SAP antibodies immobilized on such beads, selectively took up fibronectin and C4-binding protein from whole normal human serum. Sepharose 78-87 fibronectin 1 Homo sapiens 184-195 6793685-7 1981 Experiments with isolated fibronectin and SAP complexed by anti-SAP-Sepharose indicated that close association of pairs of SAP molecules was required for fibronectin to be bound and that each SAP dimer was capable of taking up a single molecule of fibronectin. Sepharose 68-77 amyloid P component, serum Homo sapiens 42-45 6793685-7 1981 Experiments with isolated fibronectin and SAP complexed by anti-SAP-Sepharose indicated that close association of pairs of SAP molecules was required for fibronectin to be bound and that each SAP dimer was capable of taking up a single molecule of fibronectin. Sepharose 68-77 amyloid P component, serum Homo sapiens 64-67 6793685-7 1981 Experiments with isolated fibronectin and SAP complexed by anti-SAP-Sepharose indicated that close association of pairs of SAP molecules was required for fibronectin to be bound and that each SAP dimer was capable of taking up a single molecule of fibronectin. Sepharose 68-77 amyloid P component, serum Homo sapiens 64-67 6793685-7 1981 Experiments with isolated fibronectin and SAP complexed by anti-SAP-Sepharose indicated that close association of pairs of SAP molecules was required for fibronectin to be bound and that each SAP dimer was capable of taking up a single molecule of fibronectin. Sepharose 68-77 fibronectin 1 Homo sapiens 154-165 6793685-7 1981 Experiments with isolated fibronectin and SAP complexed by anti-SAP-Sepharose indicated that close association of pairs of SAP molecules was required for fibronectin to be bound and that each SAP dimer was capable of taking up a single molecule of fibronectin. Sepharose 68-77 amyloid P component, serum Homo sapiens 64-67 6793685-7 1981 Experiments with isolated fibronectin and SAP complexed by anti-SAP-Sepharose indicated that close association of pairs of SAP molecules was required for fibronectin to be bound and that each SAP dimer was capable of taking up a single molecule of fibronectin. Sepharose 68-77 fibronectin 1 Homo sapiens 154-165 6168693-2 1981 This INCA is based on demonstrating that C1s and C1-inhibitor (C1-In) are linked: after an incubation with anti-C1s-Sepharose, bound C1sC1-In complexes are detected by 125I-anti-C1-In. Sepharose 116-125 caspase recruitment domain family member 17 Homo sapiens 5-9 6168693-2 1981 This INCA is based on demonstrating that C1s and C1-inhibitor (C1-In) are linked: after an incubation with anti-C1s-Sepharose, bound C1sC1-In complexes are detected by 125I-anti-C1-In. Sepharose 116-125 complement C1s Homo sapiens 41-44 6168693-2 1981 This INCA is based on demonstrating that C1s and C1-inhibitor (C1-In) are linked: after an incubation with anti-C1s-Sepharose, bound C1sC1-In complexes are detected by 125I-anti-C1-In. Sepharose 116-125 serpin family G member 1 Homo sapiens 49-61 6168693-2 1981 This INCA is based on demonstrating that C1s and C1-inhibitor (C1-In) are linked: after an incubation with anti-C1s-Sepharose, bound C1sC1-In complexes are detected by 125I-anti-C1-In. Sepharose 116-125 serpin family G member 1 Homo sapiens 63-68 6168693-2 1981 This INCA is based on demonstrating that C1s and C1-inhibitor (C1-In) are linked: after an incubation with anti-C1s-Sepharose, bound C1sC1-In complexes are detected by 125I-anti-C1-In. Sepharose 116-125 complement C1s Homo sapiens 112-115 6168693-2 1981 This INCA is based on demonstrating that C1s and C1-inhibitor (C1-In) are linked: after an incubation with anti-C1s-Sepharose, bound C1sC1-In complexes are detected by 125I-anti-C1-In. Sepharose 116-125 serpin family G member 1 Homo sapiens 136-141 7038851-2 1981 Intact IgG, F(ab")2 gamma and Fab gamma of RapA inhibited the binding of protein-A-reactive 125I-IgE, whereas only intact IgG and Fc gamma fragments from both RapA and NRG inhibited the binding of 125I-Fc gamma to protein A-Sepharose. Sepharose 224-233 transcriptional regulating factor 1 Homo sapiens 43-47 6975125-0 1981 Temperature-sensitive binding of solid phase C1q to aggregated human immunoglobulin G. The first component of complement (C1q) coupled to Sepharose by cyanogen bromide was found not to bind aggregated human gamma-globulin or immune complexes at room temperature, whereas at 4 degrees C binding was nearly complete. Sepharose 138-147 complement C1q A chain Homo sapiens 122-125 6976931-3 1981 The precipitation of C1q by the SO4(2-) groups in agarose gels was used as a means to rapidly monitor the elution of C1q. Sepharose 50-57 complement C1q A chain Homo sapiens 21-24 6976931-3 1981 The precipitation of C1q by the SO4(2-) groups in agarose gels was used as a means to rapidly monitor the elution of C1q. Sepharose 50-57 complement C1q A chain Homo sapiens 117-120 7028120-3 1981 A rapid purification of small amounts of proteinase B was achieved by affinity chromatography on the nitrated proteinase B inhibitor, immobilized on CH-Sepharose according to Bunning and Holzer (Bunning, P. and Holzer, H. (1977). Sepharose 152-161 proteinase B Saccharomyces cerevisiae S288C 41-53 7021559-1 1981 Human placenta insulin receptor was purified 11,000-fold to near homogeneity using DEAE-cellulose chromatography and affinity chromatography on insulin-Sepharose. Sepharose 152-161 insulin receptor Homo sapiens 15-31 6798971-2 1981 Frog epidermis tyrosinase was coupled to Sepharose activated with low concentrations of CNBr. Sepharose 41-50 tyrosinase Homo sapiens 15-25 6170512-2 1981 The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast, reacts immunologically with interferon-beta separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and absorbs interferon-beta immunologically when bound to CNBr-activated Sepharose. Sepharose 355-364 interferon beta 1 Homo sapiens 54-75 7346232-2 1981 Matrices with excellent capacity and stability were obtained by coupling collagen-binding fragments of fibronectin to Sepharose. Sepharose 118-127 fibronectin 1 Homo sapiens 103-114 6912277-11 1981 Organomercurial agarose was employed for the rapid removal of sulfhydryl-bearing, hemolytically inactive forms of C3 and C3b from native hemolytically active C3. Sepharose 16-23 complement C3 Homo sapiens 121-124 6167633-4 1981 M1/42-coupled Sepharose-4B beads were used to purify H-2d antigens by affinity chromatography. Sepharose 14-23 UDP glucuronosyltransferase 2 family, polypeptide B5 Mus musculus 0-5 6790617-3 1981 A similar protein is precipitated by transferrin-agarose, but not by agarose alone. Sepharose 49-56 transferrin Homo sapiens 37-48 6790617-4 1981 Peptide mapping by limited proteolysis shows that the proteins precipitated by OKT-9 antibodies and transferrin-agarose are homologous. Sepharose 112-119 transferrin Homo sapiens 100-111 6271996-0 1981 Purification of tonin by chromatography using soybean trypsin inhibitor coupled CH-sepharose 4B. Sepharose 83-92 kallikrein 1-related peptidase C2 Rattus norvegicus 16-21 6271996-1 1981 Tonin was purified from rat submaxillary gland homogenates by affinity chromatography on soybean trypsin inhibitor coupled CH-Sepharose 4B and two additional steps of conventional chromatography. Sepharose 126-138 kallikrein 1-related peptidase C2 Rattus norvegicus 0-5 6271996-2 1981 The use of affinity chromatography by soybean trypsin inhibitor coupled CH-Sepharose 4B permits a new approach in the purification of tonin, since it can completely separate in one step troublesome contamination of the enzymes which showed tosyl-L-arginine methyl ester hydrochloride esterase activity. Sepharose 75-84 kallikrein 1-related peptidase C2 Rattus norvegicus 134-139 6795738-4 1981 The apparent molecular weight of this form of factor VIII is significantly lower than that of the forms of factor VIII which contain platelet aggregating activity, and it exhibits a higher mobility on agarose electrophoresis. Sepharose 201-208 coagulation factor VIII Bos taurus 46-57 6795738-4 1981 The apparent molecular weight of this form of factor VIII is significantly lower than that of the forms of factor VIII which contain platelet aggregating activity, and it exhibits a higher mobility on agarose electrophoresis. Sepharose 201-208 coagulation factor VIII Bos taurus 107-118 7263682-2 1981 For rapid simple purification of terminal deoxynucleotidyltransferase from limited amounts of tissues, we have developed an immunoadsorbent column chromatographic method using antiterminal transferase antibody-conjugated Sepharose 4B. Sepharose 221-233 DNA nucleotidylexotransferase Bos taurus 33-69 6267040-1 1981 Myosin light chain kinase and a fraction of type II cAMP-dependent protein kinase have been partially purified from bovine brain by affinity chromatography on calmodulin-Sepharose. Sepharose 170-179 calmodulin Bos taurus 159-169 6267040-3 1981 A fraction of soluble cAMP-dependent protein kinase also bound to calmodulin-Sepharose and was purified 2300-fold. Sepharose 77-86 calmodulin Bos taurus 66-76 6172049-0 1981 Purification of human basophils by affinity chromatography on anti-IgE-sepharose 6MB. Sepharose 71-80 immunoglobulin heavy constant epsilon Homo sapiens 67-70 6172049-1 1981 This work describes a method for the purification of basophil leukocytes from human peripheral blood by the use of a three-step separation technique including affinity chromatography on anti-IgE-sepharose 6MB. Sepharose 195-204 immunoglobulin heavy constant epsilon Homo sapiens 191-194 7325969-1 1981 Sucrase--isomaltase was purified from rat intestinal microvillus membranes after injection of D-[2-3H]mannose and L-[6-3H]fucose, using a column of monoclonal antibody-protein A-Sepharose. Sepharose 178-187 sucrase-isomaltase Rattus norvegicus 0-19 7325970-1 1981 Cathepsin D was purified by two-step affinity chromatography on concanavalin A-- and pepstatin--Sepharose. Sepharose 96-105 cathepsin D Sus scrofa 0-11 7248966-7 1981 Concanavalin A:Sepharose chromatography of the alpha subunit secreted by HeLa CCL 2.2 and CCL 2.1 indicated that both proteins possess oligosaccharide side chains containing mannose while chromatography of these proteins on ricin:agarose suggested that less of the alpha subunit from CCL 2.1 contains galactose than that from CCL 2.2. Sepharose 15-24 C-C motif chemokine ligand 2 Homo sapiens 78-83 6167677-1 1981 An immunosorbent column specific for the myelin basic protein (BP) was prepared by coupling purified anti-BP antibodies to cyanogen bromide (BrCN)-activated Sepharose 4B. Sepharose 157-166 myelin basic protein Bos taurus 41-61 7029526-1 1981 Antibodies to myosin light chain kinase, purified from turkey gizzard smooth muscle, were developed in rabbits and purified by affinity chromatography on a myosin light chain kinase-Sepharose 4B column. Sepharose 182-191 myosin light chain kinase, smooth muscle Meleagris gallopavo 14-39 7023544-5 1981 For purification, affinity columns of Sepharose with bound soybean trypsin inhibitor, casein and histones were successively used. Sepharose 38-47 kunitz trypsin protease inhibitor Glycine max 67-84 7249377-1 1981 The affinity of placental protein 5 (PP5) for thrombin was examined by the application of placental homogenate, late pregnancy serum, plasma and amniotic fluid to thrombin-Sepharose column chromatography. Sepharose 172-181 tissue factor pathway inhibitor 2 Homo sapiens 16-35 7249377-1 1981 The affinity of placental protein 5 (PP5) for thrombin was examined by the application of placental homogenate, late pregnancy serum, plasma and amniotic fluid to thrombin-Sepharose column chromatography. Sepharose 172-181 tissue factor pathway inhibitor 2 Homo sapiens 37-40 7032516-16 1981 Mg2+ promotes binding of yeast hexokinase to agarose-immobilized Procion Green H-4G but not to the other dyes tested. Sepharose 45-52 hexokinase Saccharomyces cerevisiae S288C 31-41 6268183-1 1981 Rhodopsin in rod outer segment was solubilized with sucrose laurylmonoester and then purified by concanavalin A-Sepharose column. Sepharose 112-121 rhodopsin Homo sapiens 0-9 7261401-4 1981 Albumin was transported by electrophoresis into the agarose gels, in which zones of immunoprecipitates were formed. Sepharose 52-59 albumin Homo sapiens 0-7 6457032-1 1981 Fibronectin was isolated from porcine plasma by affinity chromatography with gelatin-linked Sepharose 4B. Sepharose 92-101 fibronectin 1 Homo sapiens 0-11 7265171-5 1981 CSF from 30 of 40 MS patients (75%) showed 1-4 oligoclonal IgG bands in agarose gel electrophoresis (PANAGEL), however, the resolution of bands was generally poor. Sepharose 72-79 colony stimulating factor 2 Homo sapiens 0-3 7241383-1 1981 The interaction of the nicotinic acetylcholine receptor from denervated rat muscle (AChR, solubilized in Triton X-100) and Concanavalin-A (Con-A) was studied by soluble Con-A competing with the binding of AChR to agarose immobilized Con-A, by immuno-precipitation of Con-A-AChR complexes and by inhibition of alpha-bungarotoxin binding to AChR. Sepharose 213-220 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 23-55 7241383-6 1981 A sub-population of AChR prepared by affinity purification on Lens Culinaris lectin-agarose columns was 100% inhibited from toxin binding when preincubated with Con-A. Sepharose 84-91 cholinergic receptor nicotinic alpha 2 subunit Rattus norvegicus 20-24 6270664-2 1981 Antisera developed in a goat against purified human placental transferrin binding protein was purified by fractional sodium sulfate precipitation and adsorption against Sepharose-bound transferrin to remove trace anti-transferrin activity. Sepharose 169-178 transferrin Homo sapiens 62-73 6270664-2 1981 Antisera developed in a goat against purified human placental transferrin binding protein was purified by fractional sodium sulfate precipitation and adsorption against Sepharose-bound transferrin to remove trace anti-transferrin activity. Sepharose 169-178 transferrin Homo sapiens 185-196 6270664-2 1981 Antisera developed in a goat against purified human placental transferrin binding protein was purified by fractional sodium sulfate precipitation and adsorption against Sepharose-bound transferrin to remove trace anti-transferrin activity. Sepharose 169-178 transferrin Homo sapiens 185-196 6792739-2 1981 Rabbit antibodies to human factor VIII were insolubilised onto CNBr - activated Sepharose 2B which was used for the preparation of affinity columns. Sepharose 80-89 cytochrome c oxidase subunit 8A Homo sapiens 34-38 6787052-2 1981 Colonic mucin was prepared by phenol-water partition extraction of th colonic mucosa, followed by ethanol precipitation of the water-soluble material, molecular sieve chromatography, and reductive sodium dodecyl sulfate-disc gel electrophoresis in an agarose-polyacrylamide gradient gel. Sepharose 251-258 LOC100508689 Homo sapiens 8-13 7295284-5 1981 Antisera specific for the GPI-1B variant were enriched by absorbing selected sera with GPI-1A conjugated to Sepharose 4B. Sepharose 108-117 glucose-6-phosphate isomerase Oryctolagus cuniculus 26-29 6791940-3 1981 Fibronectin isolated from rheumatoid synovial fluid by affinity chromatography on gelatin--Sepharose had a polypeptide pattern similar to that of plasma fibronectin in SDS--polyacrylamide gel electrophoresis. Sepharose 91-100 fibronectin 1 Homo sapiens 0-11 6169709-1 1981 Cytochrome P-450 mRNA has been partially purified from membrane-bound polysomes of the livers of phenobarbital-treated rats by SDS-phenol-chloroform extraction, followed by poly(U)-Sepharose chromatography and by centrifugation through a sucrose density gradient. Sepharose 181-190 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 0-16 6265465-5 1981 A calcium-calmodulin-dependent protein kinase has been isolated from Xenopus oocytes using a calmodulin-Sepharose 4B affinity column. Sepharose 104-113 calmodulin-1 Xenopus laevis 10-20 6265465-5 1981 A calcium-calmodulin-dependent protein kinase has been isolated from Xenopus oocytes using a calmodulin-Sepharose 4B affinity column. Sepharose 104-113 calmodulin-1 Xenopus laevis 93-103 6164726-2 1981 Gel filtration chromatography of GW-39 extract on Sepharose 4B columns reveals that more than 90% of the CSAp is associated with the void volume fraction. Sepharose 50-59 ERCC excision repair 8, CSA ubiquitin ligase complex subunit Homo sapiens 105-109 6164726-6 1981 CSAp is a glycoprotein that binds to Sepharose-concanavalin A and is distinct from other known tumor-associated antigens in immunologic and physicochemical properties. Sepharose 37-46 ERCC excision repair 8, CSA ubiquitin ligase complex subunit Homo sapiens 0-4 6264195-2 1981 Histone H5 was demonstrated in all samples of these cells and was characterized by electrophoresis in polyacrylamide gel, extraction with perchloric acid, amino acid analysis, and immunodiffusion in agarose gel. Sepharose 199-206 H1 histone family, member 0 Gallus gallus 0-10 6785350-2 1981 When whole serum containing [125I] C3 was incubated with agarose-thioglucose, labeled C3b was taken up in a form that was not removed by 2 M NaCl but was released by 10 mM dithiothreitol. Sepharose 57-64 complement C3 Homo sapiens 86-89 7017165-5 1981 The 65K antigen is found predominantly in the nucleus and co-elutes with EBNA during partial purification by DNA-Sepharose and Blue Dextran-Sepharose chromatography. Sepharose 113-122 ubiquitin specific peptidase 39 Homo sapiens 4-7 7017165-5 1981 The 65K antigen is found predominantly in the nucleus and co-elutes with EBNA during partial purification by DNA-Sepharose and Blue Dextran-Sepharose chromatography. Sepharose 140-149 ubiquitin specific peptidase 39 Homo sapiens 4-7 7195445-0 1981 A simple two-step procedure for the simultaneous isolation of corticosteroid binding globulin and sex hormone binding globulin from human serum by chromatography on cortisol-Sepharose and phenyl-Sepharose. Sepharose 174-183 sex hormone binding globulin Homo sapiens 98-126 6943584-3 1981 After isoelectric focusing of whole mouse plasma in low-endosmosis agarose, C4-BP was demonstrated as a single precipitin band by overlaying monospecific antiserum on the agarose gel. Sepharose 171-178 complement component 4 binding protein Mus musculus 76-81 7194873-2 1981 Androgen-binding protein (ABP) was purified from caput epididymis of the rat by sequential chromatography on DEAE-Sepharose, hydroxylapatite, dihydrotestosterone-17 beta-hemisuccinyl-1,6-diaminohexane-Sepharose, and Sephadex G-150. Sepharose 114-123 sex hormone binding globulin Rattus norvegicus 0-24 7194873-2 1981 Androgen-binding protein (ABP) was purified from caput epididymis of the rat by sequential chromatography on DEAE-Sepharose, hydroxylapatite, dihydrotestosterone-17 beta-hemisuccinyl-1,6-diaminohexane-Sepharose, and Sephadex G-150. Sepharose 114-123 sex hormone binding globulin Rattus norvegicus 26-29 6260797-2 1981 Cytochrome P-450scc was isolated from mitochondria of bovine adrenal cortex by hydrophobic chromatography on octyl Sepharose followed by affinity chromatography on cholesterol-7-(thiomethyl)carboxy-3 beta-acetate-Sepharose. Sepharose 115-124 cholesterol side-chain cleavage enzyme, mitochondrial Bos taurus 0-19 6260797-4 1981 Chromatography of the octyl Sepharose eluate on CA-Sepharose removed extraneous proteins and resolved the cytochrome P-450scc into two fractions, each of which displayed monophasic binding with all three substrates. Sepharose 28-37 cholesterol side-chain cleavage enzyme, mitochondrial Bos taurus 106-125 6894553-2 1981 It was purified 130-fold with a 10% yield by anion-exchange chromatography followed by affinity chromatography on calmodulin-Sepharose. Sepharose 125-134 calmodulin Bos taurus 114-124 6453524-0 1981 Isoelectric focusing of PGM1 (E.C.2.7.5.1) on agarose. Sepharose 46-53 phosphoglucomutase 1 Homo sapiens 24-28 6453524-2 1981 A method for isoelectric focusing of phosphoglucomutase (PGM1) using agarose was found to have significant advantages over published acrylamide technics. Sepharose 69-76 phosphoglucomutase 1 Homo sapiens 57-61 7295815-0 1981 [Activation by heparin-Sepharose of prothrombin conversion to prethrombin 1]. Sepharose 23-32 coagulation factor II, thrombin Homo sapiens 36-47 7295815-1 1981 Using affinity chromatography on heparin-Sepharose, homogeneous prothrombin containing no factor Xa or thrombin was separated into three components differing in their affinities for the bioadsorbent. Sepharose 41-50 coagulation factor II, thrombin Homo sapiens 64-75 7295815-4 1981 Rechromatography of component 2 provided further evidence for prothrombin modification to prethrombin 1 by heparin-Sepharose. Sepharose 115-124 coagulation factor II, thrombin Homo sapiens 62-73 7295815-6 1981 Heparin-Sepharose probably induced changes in prothrombin conformation and the formation of a catalytic center responsible for prothrombin splitting to prethrombin 1. Sepharose 8-17 coagulation factor II, thrombin Homo sapiens 46-57 7295815-6 1981 Heparin-Sepharose probably induced changes in prothrombin conformation and the formation of a catalytic center responsible for prothrombin splitting to prethrombin 1. Sepharose 8-17 coagulation factor II, thrombin Homo sapiens 127-138 7295815-7 1981 Heparin-Sepharose can be used for separation of prothrombin proteolytic products by thrombin and for isolation of prethrombin 1 and prothrombin fragment 1. Sepharose 8-17 coagulation factor II, thrombin Homo sapiens 48-59 7295815-7 1981 Heparin-Sepharose can be used for separation of prothrombin proteolytic products by thrombin and for isolation of prethrombin 1 and prothrombin fragment 1. Sepharose 8-17 coagulation factor II, thrombin Homo sapiens 51-59 7295815-7 1981 Heparin-Sepharose can be used for separation of prothrombin proteolytic products by thrombin and for isolation of prethrombin 1 and prothrombin fragment 1. Sepharose 8-17 coagulation factor II, thrombin Homo sapiens 132-143 7021414-8 1981 Like plasma inactive renin, it could be isolated by chromatography on Cibacron blue-agarose (Affi-Gel blue). Sepharose 84-91 renin Homo sapiens 21-26 7021414-11 1981 Trypsin-activated inactive renal renin had a mw of 46,500 +/- 500; its pH optimum was identical with that of active renal renin, and it no longer bound to Cibacron blue-agarose. Sepharose 169-176 renin Homo sapiens 33-38 6971311-5 1981 The rosette-inhibiting factor in culture filtrates was specifically absorbed with IgE-coupled Sepharose and was recovered from the beads by elution at acid pH, indicating that the soluble factors have affinity for IgE. Sepharose 94-103 immunoglobulin heavy constant epsilon Homo sapiens 82-85 6971316-8 1981 Finally, Sepharose conjugated with purified IgG fractions provided on extremely reactive IL-2 absorption matrix. Sepharose 9-18 interleukin 2 Rattus norvegicus 89-93 7217669-4 1981 A weak interaction between CRP and agarose was observed, which was also CA++-dependent and could be inhibited by phosphocholine and galactose. Sepharose 35-42 C-reactive protein Homo sapiens 27-30 6113625-1 1981 Gamma-glutamyltranspeptidase (GGT) of adult rat liver was solubilized by treatment with papain and further purified by affinity column chromatography on concanavalin-A Sepharose 4B, followed by ion exchange chromatography on DEAE-cellulose. Sepharose 168-180 gamma-glutamyltransferase 1 Rattus norvegicus 0-28 6113625-1 1981 Gamma-glutamyltranspeptidase (GGT) of adult rat liver was solubilized by treatment with papain and further purified by affinity column chromatography on concanavalin-A Sepharose 4B, followed by ion exchange chromatography on DEAE-cellulose. Sepharose 168-180 gamma-glutamyltransferase 1 Rattus norvegicus 30-33 6114491-3 1981 Absorption of patients" sera with anti-human IgM (mu chain) or protein A-agarose, but not with anti-human IgG (gamma chain), decreased their ability to decrease the activity of lipomodulin on phospholipase A2 or to precipitate the radioactive lipomodulin. Sepharose 73-80 phospholipase A2 group IB Homo sapiens 192-208 6265934-1 1981 A cell surface glycoprotein antigen with an apparent molecular weight of about 100,000 that is selectively expressed on proliferating cells was purified from deoxycholate-solubilized membranes of a cultured human leukemic thymus-derived (T) cell line by affinity chromatography on a monoclonal antibody-Sepharose column. Sepharose 303-312 CD83 molecule Homo sapiens 2-27 7345564-3 1981 The isolation of TR-c from a crude bacterial sonicate involves five fractionation steps: anion exchange chromatography (DE-52 Whatman), gel filtration (Ac-A-22, Ultrogel), and affinity chromatography respectively on phenyl-Sepharose CL 4B, iminodiacetic acid-Sepharose CL 4B, and lysine-Sepharose 4B. Sepharose 223-232 tRNA-Cys (GCA) 24-1 Homo sapiens 17-21 6165382-6 1981 Complete equilibrium binding isotherms of both detergents to the protein were obtained by using MBP immobilized on agarose. Sepharose 115-122 myelin basic protein Homo sapiens 96-99 6165382-10 1981 Affinity chromatography studies indicated that, in the presence of NaDodSO4 (but not in its absence), [125I]MBP interacted with agarose-immobilized histone, lysozyme, and MBP but did not interact with ovalbumin-agarose. Sepharose 128-135 myelin basic protein Homo sapiens 108-111 6165382-10 1981 Affinity chromatography studies indicated that, in the presence of NaDodSO4 (but not in its absence), [125I]MBP interacted with agarose-immobilized histone, lysozyme, and MBP but did not interact with ovalbumin-agarose. Sepharose 211-218 myelin basic protein Homo sapiens 108-111 7018580-7 1981 Neutral alpha-glucosidase AB migrates more rapidly to the anode than alpha-glucosidase C when agarose, Cellogel, acrylamide or starch are used as support media. Sepharose 94-101 glucosidase II alpha subunit Homo sapiens 0-28 7018580-7 1981 Neutral alpha-glucosidase AB migrates more rapidly to the anode than alpha-glucosidase C when agarose, Cellogel, acrylamide or starch are used as support media. Sepharose 94-101 sucrase-isomaltase Homo sapiens 8-25 7248302-6 1981 When H2O2 and SCN- were applied to a Sepharose column bearing covalently attached lactoperoxidase, the column eluate inactivated hexokinase. Sepharose 37-46 lactoperoxidase Homo sapiens 82-97 7248302-6 1981 When H2O2 and SCN- were applied to a Sepharose column bearing covalently attached lactoperoxidase, the column eluate inactivated hexokinase. Sepharose 37-46 hexokinase 1 Homo sapiens 129-139 6162843-2 1981 Using double diffusion analysis in agarose gels, we show that anti-rat liver microsomal epoxide hydrolase forms a single precipitin line with solubilized microsomes from rat and mouse liver, but no reaction is seen with the corresponding cytosolic fractions. Sepharose 35-42 epoxide hydrolase 1 Rattus norvegicus 77-105 7195876-1 1981 The interaction between rabbit and rat androgen binding protein (ABP) and rabbit serum testosterone binding globulin (TeBG) with concanavalin A (Con A) was studied using affinity chromatography on Con A-Sepharose 4 B columns. Sepharose 203-212 sex hormone binding globulin Rattus norvegicus 65-68 7195876-2 1981 When partly purified rat ABP, equilibrated with [3H]5 alpha-dihydrotesterone [3H]DHT was applied to Con A-Sepharose columns, approximately 50% of the ABP was retained by the column, whereas the remaining was eluted with the break-through protein fraction. Sepharose 106-115 sex hormone binding globulin Rattus norvegicus 25-28 6788753-2 1981 In order to increase the specificity of the assay, an antiserum against whole hCG was purified on a column of hCG beta carboxyl-terminal peptide (residues 123-145) covalently linked to Sepharose 4B. Sepharose 185-194 chorionic gonadotropin subunit beta 5 Homo sapiens 78-81 6788753-2 1981 In order to increase the specificity of the assay, an antiserum against whole hCG was purified on a column of hCG beta carboxyl-terminal peptide (residues 123-145) covalently linked to Sepharose 4B. Sepharose 185-194 chorionic gonadotropin subunit beta 5 Homo sapiens 110-113 7017622-5 1981 Some of the NT-like immunoreactivity could also be bound to an retrieved from anti-NT-antibody-Sepharose preparations. Sepharose 95-104 neurotensin Bos taurus 12-14 7017622-5 1981 Some of the NT-like immunoreactivity could also be bound to an retrieved from anti-NT-antibody-Sepharose preparations. Sepharose 95-104 neurotensin Bos taurus 83-85 7226170-0 1981 Agarose-linked xanthosine: a biospecific resin for guanine aminohydrolase. Sepharose 0-7 guanine deaminase Homo sapiens 51-73 7226527-1 1981 When compared to values obtained in healthy normolipidemic normal weight control subjects, the plasma antithrombin III level determined by immunological, clotting and thrombin-agarose diffusion techniques was found to be obviously decreased in patients with decompensated cirrhosis of the liver and slightly but significantly increased in hyperlipidemic and especially in hypertriglyceridemic subjects. Sepharose 176-183 serpin family C member 1 Homo sapiens 102-118 7226527-1 1981 When compared to values obtained in healthy normolipidemic normal weight control subjects, the plasma antithrombin III level determined by immunological, clotting and thrombin-agarose diffusion techniques was found to be obviously decreased in patients with decompensated cirrhosis of the liver and slightly but significantly increased in hyperlipidemic and especially in hypertriglyceridemic subjects. Sepharose 176-183 coagulation factor II, thrombin Homo sapiens 106-114 7305955-1 1981 Two isoenzyme of beta-glucuronidase from a rat basophil leukaemia tumour were co-purified 4067-fold by (NH4)2SO4 precipitation and sequential chromatography on concanavalin A--Sepharose, Sephadex G-200, DEAE-cellulose, CM-cellulose and phosphocellulose. Sepharose 176-185 glucuronidase, beta Rattus norvegicus 17-35 7462631-3 1981 SAP undergoes calcium-dependent binding to zymosan, agarose, and amyloid fibrils, but its functional properties are not yet known. Sepharose 52-59 amyloid P component, serum Bos taurus 0-3 7462631-7 1981 SAP thus seems to react with fixed C3 in a manner similar to the reaction of C3 with bovine conglutinin, a molecule that also undergoes calcium-dependent binding to zymosan and agarose. Sepharose 177-184 amyloid P component, serum Bos taurus 0-3 6165014-3 1981 A purification process for IFN-gamma was developed consisting of sequential chromatographic separations on controlled-pore glass, concanavalin A-Sepharose, and Bio-Gel P-200. Sepharose 145-154 interferon gamma Homo sapiens 27-36 28305355-1 1981 Fibronectin, with a subunit molecular weight of 220,000 daltons, was isolated from the ovary of the sea urchin,Pseudocentrotus depressus, using affinity chromatography on heat-denatured mammalian collagen coupled to Sepharose 4B. Sepharose 216-225 fibronectin 1 Homo sapiens 0-11 6452159-2 1981 Myosin can be separated from other proteins by gel filtration on a Sepharose 4B--agarose column. Sepharose 67-76 myosin heavy chain 14 Homo sapiens 0-6 6452159-2 1981 Myosin can be separated from other proteins by gel filtration on a Sepharose 4B--agarose column. Sepharose 81-88 myosin heavy chain 14 Homo sapiens 0-6 6256381-4 1981 A GnRH analog, D-Lys6-GnRH (to which a small quantity of [125I]iodoTyr5-D-Lys6-GnRH was added), was coupled by its epsilon-amino group with an N-hydroxysuccinimide ester then, through a 10-A spacer arm, to a cross-linked agarose matrix. Sepharose 221-228 gonadotropin releasing hormone 1 Homo sapiens 2-6 6256381-4 1981 A GnRH analog, D-Lys6-GnRH (to which a small quantity of [125I]iodoTyr5-D-Lys6-GnRH was added), was coupled by its epsilon-amino group with an N-hydroxysuccinimide ester then, through a 10-A spacer arm, to a cross-linked agarose matrix. Sepharose 221-228 gonadotropin releasing hormone 1 Homo sapiens 22-26 6256381-4 1981 A GnRH analog, D-Lys6-GnRH (to which a small quantity of [125I]iodoTyr5-D-Lys6-GnRH was added), was coupled by its epsilon-amino group with an N-hydroxysuccinimide ester then, through a 10-A spacer arm, to a cross-linked agarose matrix. Sepharose 221-228 gonadotropin releasing hormone 1 Homo sapiens 22-26 6455773-0 1981 Binding of plasmin(ogen) to sepharose bound fibrin(ogen) alpha-chain. Sepharose 28-37 fibrinogen alpha chain Homo sapiens 44-68 7213594-10 1981 Retention by concanavalin A-Sepharose was observed only after treatment of the oligosaccharide with beta-galactosidase. Sepharose 28-37 galactosidase beta 1 Homo sapiens 100-118 7030313-2 1981 Affinity chromatography of fragments, released from fibronectin by limited proteolysis with trypsin, chymotrypsin and subtilisin, on actin-Sepharose and other protein-Sepharose columns was used to locate the binding site. Sepharose 139-148 fibronectin 1 Gallus gallus 52-63 7030313-2 1981 Affinity chromatography of fragments, released from fibronectin by limited proteolysis with trypsin, chymotrypsin and subtilisin, on actin-Sepharose and other protein-Sepharose columns was used to locate the binding site. Sepharose 167-176 fibronectin 1 Gallus gallus 52-63 7305945-2 1981 S-Adenosyl-L-homocysteine hydrolase has been purified to apparent homogeneity from rat liver by means of affinity chromatography on 8-(3-aminopropylamino)adenosine linked to Sepharose. Sepharose 174-183 adenosylhomocysteinase Rattus norvegicus 0-35 6165511-2 1981 A kallikrein-like enzyme in plasma of patients with acute pancreatitis was further purified by successive hydroxyapatite/cellulose and Sepharose-4B column chromatography. Sepharose 135-144 kallikrein related peptidase 4 Homo sapiens 2-12 6258801-1 1981 Taking advantage of the known specificity of interaction of HMG 14 and 17 with actively transcribed chromatin, we have covalently cross-linked these proteins to agarose and used this HMG 14-17 column to purify active nucleosomes. Sepharose 161-168 high mobility group nucleosome binding domain 1 Gallus gallus 60-66 7451992-8 1981 Putative receptor obtained by elution from IgG2a-Sepharose rebound equally well to IgG2a-, IgG1- and IgG2b-Sepharose, but did not rebind to IgG3-, F(ab")2-, or BSA-Sepharose. Sepharose 49-58 immunoglobulin heavy variable V1-9 Mus musculus 43-48 7451992-8 1981 Putative receptor obtained by elution from IgG2a-Sepharose rebound equally well to IgG2a-, IgG1- and IgG2b-Sepharose, but did not rebind to IgG3-, F(ab")2-, or BSA-Sepharose. Sepharose 49-58 LOC105243590 Mus musculus 91-95 7451992-8 1981 Putative receptor obtained by elution from IgG2a-Sepharose rebound equally well to IgG2a-, IgG1- and IgG2b-Sepharose, but did not rebind to IgG3-, F(ab")2-, or BSA-Sepharose. Sepharose 49-58 immunoglobulin heavy constant gamma 2B Mus musculus 101-106 7451992-8 1981 Putative receptor obtained by elution from IgG2a-Sepharose rebound equally well to IgG2a-, IgG1- and IgG2b-Sepharose, but did not rebind to IgG3-, F(ab")2-, or BSA-Sepharose. Sepharose 107-116 immunoglobulin heavy variable V1-9 Mus musculus 43-48 7451992-8 1981 Putative receptor obtained by elution from IgG2a-Sepharose rebound equally well to IgG2a-, IgG1- and IgG2b-Sepharose, but did not rebind to IgG3-, F(ab")2-, or BSA-Sepharose. Sepharose 107-116 immunoglobulin heavy constant gamma 2B Mus musculus 101-106 7451992-8 1981 Putative receptor obtained by elution from IgG2a-Sepharose rebound equally well to IgG2a-, IgG1- and IgG2b-Sepharose, but did not rebind to IgG3-, F(ab")2-, or BSA-Sepharose. Sepharose 107-116 immunoglobulin heavy variable V1-9 Mus musculus 43-48 7451992-8 1981 Putative receptor obtained by elution from IgG2a-Sepharose rebound equally well to IgG2a-, IgG1- and IgG2b-Sepharose, but did not rebind to IgG3-, F(ab")2-, or BSA-Sepharose. Sepharose 107-116 immunoglobulin heavy constant gamma 2B Mus musculus 101-106 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 61-70 immunoglobulin heavy constant gamma 2B Mus musculus 55-60 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 61-70 immunoglobulin heavy constant gamma 2B Mus musculus 87-92 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 61-70 immunoglobulin heavy variable V1-9 Mus musculus 95-100 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 61-70 LOC105243590 Mus musculus 106-110 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 61-70 immunoglobulin heavy constant gamma 2B Mus musculus 87-92 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 61-70 immunoglobulin heavy variable V1-9 Mus musculus 145-150 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 61-70 LOC105243590 Mus musculus 156-160 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 111-120 immunoglobulin heavy constant gamma 2B Mus musculus 55-60 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 111-120 LOC105243590 Mus musculus 106-110 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 111-120 immunoglobulin heavy constant gamma 2B Mus musculus 55-60 7451992-9 1981 Similarly, Fc gamma-binding macromolecules eluted from IgG2b-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose rebound only to IgG2b-, IgG2a- and IgG1-Sepharose. Sepharose 111-120 LOC105243590 Mus musculus 106-110 7452092-3 1981 Solubilized platelet proteins were examined by crossed immunoelectrophoresis against polyspecific antiplatelet antibodies, and the interaction with thrombin observed by insertion of an intermediate gel containing thrombin coupled to Sepharose 4-B. Sepharose 233-246 coagulation factor II, thrombin Homo sapiens 148-156 7311079-0 1981 Fractionation of IgG, IgG2a, IgG2b and IgG3 immunoglobulins from mouse serum by protein A-sepharose column chromatography. Sepharose 90-99 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 39-43 6940121-3 1981 Insulin receptors were concentrated and purified 20-fold by chromatography of the cell extract on wheat germ agglutinin-agarose, and then specifically precipitated by receptor antibodies after addition of a second antibody. Sepharose 120-127 insulin Homo sapiens 0-7 7013005-4 1981 Using lectin-Sepharose affinity chromatography and immune precipitation with specific anti-glycophorin A antiserum followed by polyacrylamide slab gel electrophoresis a precursor of glycophorin A was visualized. Sepharose 13-22 glycophorin A (MNS blood group) Homo sapiens 182-195 6162654-10 1981 Finally, as earlier found for 48/80, somatostatin attached to Sepharose columns retarded the passage of mast cells and elicited histamine release indicating an action at the cell surface. Sepharose 62-71 somatostatin Homo sapiens 37-49 7305977-1 1981 A rat liver mannan-binding protein was isolated by affinity chromatography on invertase--Sepharose by a modification of the method of Kawasaki, Etoh & Yamashina [(1978) Biochem. Sepharose 89-98 mannose binding lectin 1 Rattus norvegicus 12-34 7451445-2 1981 The heparin-inhibitable lectin activity from adult chicken liver and embryonic chick pectoral muscle has been purified by gel filtration on Sepharose CL-2B followed by affinity chromatography on heparin-Sepharose. Sepharose 140-149 galectin 3 Gallus gallus 24-30 6940440-6 1981 SDS-PAGE analysis of immunoprecipitates formed between ATS and radiolabeled cell-surface components of human thymocytes demonstrated that ATS bound a 48,000-molecular-weight component that could be adsorbed to Sepharose 4B coupled with Les culinaris hemagglutinin and could be labeled by periodate-tritiated borohydride, indicating that HTA is a sialoglycoprotein. Sepharose 210-222 solute carrier family 2 member 10 Homo sapiens 138-141 6791545-0 1981 An agarose gel method for evaluating F VIII procoagulant electrophoretic distribution. Sepharose 3-10 coagulation factor VIII Homo sapiens 37-43 7347585-2 1981 Both crude preparations showed Fc receptor activity, assayed by EA rosette test and by binding to IgG-Sepharose. Sepharose 102-111 immunoglobulin superfamily member 1 Gallus gallus 31-42 7305916-3 1981 Binding of C3 to Sepharose-trypsin is saturable, with a maximum of 25-26 molecules of C3b bound per molecule of trypsin. Sepharose 17-26 complement C3 Homo sapiens 86-89 6111425-1 1981 Cytochrome P-450, NADPH-cytochrome P-450 reductase, and glucuronyltransferase were immobilized simultaneously on cyanogen bromide-activated Sepharose from phenobarbital-induced rabbit liver microsomes. Sepharose 140-149 cytochrome P-450 Oryctolagus cuniculus 0-16 6162638-4 1981 However, ovalbumin, which is transferred across the endoplasmic reticulum in the presence of tunicamycin and which is indistinguishable by immunoprecipitation, by two-dimensional gel electrophoresis and by concanavalin-A--Sepharose binding from the cytosolic form, is still secreted. Sepharose 222-231 ovalbumin (SERPINB14) Gallus gallus 9-18 6971771-3 1981 Inhibition assay of 125I-C1q binding to IgG-p-azobenzamidoethyl Sepharose 6B (IgG-Sepharose) by immune complexes was developed for the detection of circulating soluble immune complexes in the liver disease and was compared with polyclonal rheumatoid factor (pRF) binding inhibition assay and with C1q binding assay. Sepharose 64-73 complement C1q A chain Homo sapiens 25-28 6971771-3 1981 Inhibition assay of 125I-C1q binding to IgG-p-azobenzamidoethyl Sepharose 6B (IgG-Sepharose) by immune complexes was developed for the detection of circulating soluble immune complexes in the liver disease and was compared with polyclonal rheumatoid factor (pRF) binding inhibition assay and with C1q binding assay. Sepharose 64-73 complement C1q A chain Homo sapiens 297-300 7262818-7 1981 Terminal "delta 6-desaturase" was purified from rat liver microsomes for the first time by Triton X-100 solubilization, DEAE-cellulose, CM-Sephadex and cytochrome b5-Sepharose chromatography using its high affinity for cytochrome b5. Sepharose 166-175 fatty acid desaturase 2 Rattus norvegicus 10-28 7262818-7 1981 Terminal "delta 6-desaturase" was purified from rat liver microsomes for the first time by Triton X-100 solubilization, DEAE-cellulose, CM-Sephadex and cytochrome b5-Sepharose chromatography using its high affinity for cytochrome b5. Sepharose 166-175 cytochrome b5 type A Rattus norvegicus 152-165 6459984-3 1981 Using agarose thin layer or acetate foil electrophoresis the variant is represented only by a minimal cathodic broadening of the PGM1 1 band and therefore it is easily overlooked. Sepharose 6-13 phosphoglucomutase 1 Homo sapiens 129-133 6164732-3 1981 Barley beta-amylase absorbed on the Sepharose-immobilized IgG fraction of the anti-barley beta-amylase immune serum was used in this study. Sepharose 36-45 1,4-alpha-D-glucan maltohydrolase Hordeum vulgare 7-19 6164732-3 1981 Barley beta-amylase absorbed on the Sepharose-immobilized IgG fraction of the anti-barley beta-amylase immune serum was used in this study. Sepharose 36-45 1,4-alpha-D-glucan maltohydrolase Hordeum vulgare 90-102 7264318-1 1981 Affinity chromatography of human plasma, using either gelatin or fibrinogen coupled to Sepharose 4B, depletes the plasma of fibronectin. Sepharose 87-99 fibronectin 1 Homo sapiens 124-135 6778941-2 1981 Human VIII: C has been partially purified by immunoabsorbent chromatography and agarose gel filtration in the presence of 2 mM DFP. Sepharose 80-87 cytochrome c oxidase subunit 8A Homo sapiens 6-10 7024504-2 1981 Acrosin was extracted from boar sperm and purified by Sephadex gel filtration and affinity chromatography on Phe-Phe-Arg Sepharose 4B in acidic condition. Sepharose 121-133 acrosin Homo sapiens 0-7 7035844-6 1981 Moreover, when MAF-rich fraction was applied to a Con A-Sepharose column, a fraction that was adsorbed on Con A and eluted with 0.1 M alpha-methyl D-mannoside exhibited MAF activity. Sepharose 56-65 avian musculoaponeurotic fibrosarcoma oncogene homolog Mus musculus 15-18 7035844-6 1981 Moreover, when MAF-rich fraction was applied to a Con A-Sepharose column, a fraction that was adsorbed on Con A and eluted with 0.1 M alpha-methyl D-mannoside exhibited MAF activity. Sepharose 56-65 avian musculoaponeurotic fibrosarcoma oncogene homolog Mus musculus 169-172 6117834-4 1981 Practically all of the GGT contained in extracts prepared with Triton X-100 from fetal, regenerating, pre-neoplastic and neoplastic rat liver as well as that from normal adult liver bound to Con A-Sepharose and was eluted from the columns with methyl-alpha-D-mannopyranoside. Sepharose 197-206 gamma-glutamyltransferase 1 Rattus norvegicus 23-26 6117834-6 1981 We conclude that if variants of rat liver GGT exist, they cannot be reliable separated by simple affinity chromatography on Con A-Sepharose of Agarose columns. Sepharose 130-139 gamma-glutamyltransferase 1 Rattus norvegicus 42-45 6117834-6 1981 We conclude that if variants of rat liver GGT exist, they cannot be reliable separated by simple affinity chromatography on Con A-Sepharose of Agarose columns. Sepharose 143-150 gamma-glutamyltransferase 1 Rattus norvegicus 42-45 6170951-4 1981 Conversely, by employing an immunosorbent column of Sepharose-purified L-AP, one can remove the component carrying the antigenic determinants shared with L-AP, B-APa and K-AP. Sepharose 52-61 LAP Homo sapiens 71-75 6170951-4 1981 Conversely, by employing an immunosorbent column of Sepharose-purified L-AP, one can remove the component carrying the antigenic determinants shared with L-AP, B-APa and K-AP. Sepharose 52-61 LAP Homo sapiens 154-158 6163148-1 1981 Pregnancy-associated plasma protein-A (PAPP-A) was purified to homogenicity from third-trimester pregnancy plasma using ion exchange chromatography and affinity chromatography on con A-Sepharose and anti-human serum-Sepharose. Sepharose 185-194 pappalysin 1 Homo sapiens 0-37 6163148-1 1981 Pregnancy-associated plasma protein-A (PAPP-A) was purified to homogenicity from third-trimester pregnancy plasma using ion exchange chromatography and affinity chromatography on con A-Sepharose and anti-human serum-Sepharose. Sepharose 185-194 pappalysin 1 Homo sapiens 39-45 6163148-1 1981 Pregnancy-associated plasma protein-A (PAPP-A) was purified to homogenicity from third-trimester pregnancy plasma using ion exchange chromatography and affinity chromatography on con A-Sepharose and anti-human serum-Sepharose. Sepharose 216-225 pappalysin 1 Homo sapiens 0-37 6163148-1 1981 Pregnancy-associated plasma protein-A (PAPP-A) was purified to homogenicity from third-trimester pregnancy plasma using ion exchange chromatography and affinity chromatography on con A-Sepharose and anti-human serum-Sepharose. Sepharose 216-225 pappalysin 1 Homo sapiens 39-45 6306643-3 1981 Gel filtration on acrylamide agarose (AcA-22) at 21 degrees C in the absence of transferrin indicates that the transferrin-binding protein has a Stokes" radius of 4.6 nm. Sepharose 29-36 small nucleolar RNA, H/ACA box 22 Homo sapiens 38-44 6306643-3 1981 Gel filtration on acrylamide agarose (AcA-22) at 21 degrees C in the absence of transferrin indicates that the transferrin-binding protein has a Stokes" radius of 4.6 nm. Sepharose 29-36 transferrin Homo sapiens 111-122 6941248-6 1981 Chromatography of 2 M urea-solubilized coated vesicles on a calmodulin-Sepharose column demonstrated a Ca2+-dependent interaction of coated vesicle proteins and calmodulin. Sepharose 71-80 calmodulin 1 Homo sapiens 60-70 6941248-6 1981 Chromatography of 2 M urea-solubilized coated vesicles on a calmodulin-Sepharose column demonstrated a Ca2+-dependent interaction of coated vesicle proteins and calmodulin. Sepharose 71-80 calmodulin 1 Homo sapiens 161-171 7279972-1 1981 An affinity chromatography system, using haptoglobin bound covalently to Sepharose 4B, has been developed to purify human hemoglobin from soluble non-heme proteins. Sepharose 73-82 haptoglobin Homo sapiens 41-52 7279972-2 1981 Agarose-haptoglobin specifically binds hemoglobin. Sepharose 0-7 haptoglobin Homo sapiens 8-19 7279972-3 1981 Globin chains were eluted from the agarose-haptoglobin after removal of the heme. Sepharose 35-42 haptoglobin Homo sapiens 43-54 7466316-1 1981 During routine investigation of plasma proteins by agarose gel electrophoresis, a double fibrinogen band was found in a 79-year-old woman without previous history or clinical symptoms of bleeding tendency. Sepharose 51-58 fibrinogen beta chain Homo sapiens 89-99 6164178-1 1981 Amy2 phenotypes were determined by agarose gel electrophoresis on samples from 590 unrelated individuals. Sepharose 35-42 amylase alpha 2A Homo sapiens 0-4 6781095-3 1980 Affinity chromatography on antithrombin-Sepharose separated a distinct high-affinity fraction (4-5% of the total material). Sepharose 40-49 serpin family C member 1 Homo sapiens 27-39 7213665-5 1980 The data obtained from affinity chromatography of these fractions on antithrombin III-Sepharose also substantiated the observed difference in anticoagulant activity. Sepharose 86-95 serpin family C member 1 Homo sapiens 69-85 6163790-4 1980 Both estrone and estradiol proved efficient as elution agents to free AFP bound to the estradiol-Sepharose beads, but higher yields were produced with estrone. Sepharose 97-106 alpha fetoprotein Mus musculus 70-73 6260151-1 1980 Adenosine kinase (ATP:adenosine 5"-phosphotransferase, EC 2.7.1.20) has been purified to apparent homogeneity from rat brain by (NH4)2SO4 fractionation, affinity chromatography on AMP-Sepharose 4B, gel filtration with Sephadex G-100, and DE-52 cellulose column chromatography. Sepharose 184-196 adenosine kinase Rattus norvegicus 0-16 6777374-3 1980 The two phosphorylase kinase isozymes could be separated by affinity chromatography on a calmodulin-Sepharose 4B column. Sepharose 100-112 calmodulin Oryctolagus cuniculus 89-99 7236660-3 1980 Antithrombin III, the major contaminant after affinity chromatography with heparin-Sepharose 4B, could be removed by gel filtration on Bio-Gel A-5m. Sepharose 83-95 serpin family C member 1 Homo sapiens 0-16 7004726-2 1980 Plasma prorenin (inactive renin), which accounts for about 70% of the total renin in human plasma, was almost completely separated from active renin by affinity chromatography on Cibacron blue F3G-A-agarose. Sepharose 199-206 renin Homo sapiens 10-15 7004726-3 1980 The slight residual renin activity present in the prorenin peak can be removed on concanavalin A-Sepharose, demonstrating that prorenin is completely inactive. Sepharose 97-106 renin Homo sapiens 20-25 7004726-8 1980 Trypsin-activable renin from both renal extract and renal perfusate was, like plasma prorenin, almost completely separated from active renin on Cibacron blue F3G-A-agarose. Sepharose 164-171 renin Homo sapiens 18-23 7460935-0 1980 Globin-chain affinity chromatography on Sepharose-haptoglobin: a new method of study of hemoglobin synthesis in reticulocytes, in bone marrow and in colonies of erythroid precursors. Sepharose 40-49 haptoglobin Homo sapiens 50-61 7460935-1 1980 In the present work we have developed an affinity chromatography system, using haptoglobin bound covalently to Sepharose 4B, to purify hemoglobin from soluble non-heme proteins. Sepharose 111-123 haptoglobin Homo sapiens 79-90 7460935-4 1980 Globin chains can be eluted from the Sepharose-haptoglobin after removal of the heme. Sepharose 37-46 haptoglobin Homo sapiens 47-58 6257728-1 1980 Calmodulin has been purified from cell bodies of the green alga Chlamydomonas by Ca++-dependent affinity chromatography on fluphenazine-Sepharose 4B. Sepharose 136-145 calmodulin Bos taurus 0-10 7440713-2 1980 ApoE was purified from the very low density lipoproteins of hypertriglyceridemic patients by heparin-agarose affinity chromatography, DEAE-cellulose chromatography, and preparative polyacrylamide gel electrophoresis. Sepharose 101-108 apolipoprotein E Homo sapiens 0-4 7440713-13 1980 The lipoprotein distribution of apoE was investigated by agarose column chromatography and ultracentrifugation of plasma. Sepharose 57-64 apolipoprotein E Homo sapiens 32-36 7440713-14 1980 Agarose column chromatography demonstrated that all or nearly all plasma apoE is associated with lipoproteins. Sepharose 0-7 apolipoprotein E Homo sapiens 73-77 7255863-3 1980 Alanine aminotransferase and aspartate aminotransferase from porcine heart also bound to Cibacron Blue F3GA-agarose. Sepharose 108-115 AL522_RS15520 Pantoea agglomerans 29-55 6972358-5 1980 Disproportionation of GM-DF and GM-CSF was observed in ES fractions obtained using concanavalin-A/Sepharose chromatography: none of the GM-DF bound to this matrix, whereas 40% of the GM-CSF bound and was eluted with competing alpha methylglucopyranoside. Sepharose 98-107 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 32-38 7459381-2 1980 High molecular weight water-soluble mucin was separated from low molecular weight proteins by gel exclusion chromatography on Sepharose 4B, and was further separated into two major mucin fractions and several non-mucin fractions on DEAE-cellulose. Sepharose 126-138 solute carrier family 13 member 2 Rattus norvegicus 36-41 7430111-1 1980 A protease-resistant functional polypeptide domain of fibronectin that contains a heparin-binding site has been purified from chick cellular fibronectin following pronase digestion, heparin-Sepharose affinity chromatography, and molecular sieve chromatography on Sephacryl S-200. Sepharose 190-199 fibronectin 1 Gallus gallus 54-65 6108169-3 1980 Treatment of these enzymes with neuraminidase eliminated the differences in their electrophoretic migration on polyacrylamide gels and on agarose immunoelectrophoresis gels. Sepharose 138-145 neuraminidase 1 Homo sapiens 32-45 7002370-0 1980 Enzyme immunoassay for insulin with a novel separation method using activated thiol-Sepharose. Sepharose 84-93 insulin Homo sapiens 23-30 7437475-6 1980 Sepharose-linked transferrin was found to be capable of delivering 59Fe to the reticulocytes. Sepharose 0-9 transferrin Rattus norvegicus 17-28 6165352-1 1980 Cathepsin H was purified from human liver by a method involving autolysis and acetone fractionation, and chromatography on DEAE-cellulose, Ultrogel AcA 54, hydroxyapatite and concanavalin A-Sepharose. Sepharose 190-199 cathepsin H Homo sapiens 0-11 6165352-3 1980 Cathepsin H was shown to consist of a single polypeptide chain of 28 000 mol.wt., and affinity for concanavalin A-Sepharose indicated that it was a glycoprotein. Sepharose 114-123 cathepsin H Homo sapiens 0-11 7259211-1 1980 Glucokinase from dog liver has been purified to homogeneity by a procedure involving DEAE-cellulose chromatography, ammonium sulfate fractionation, gel filtration chromatography, and affinity chromatography on glucosamine-Sepharose. Sepharose 222-231 hexokinase-4 Canis lupus familiaris 0-11 7011523-1 1980 Agarose and/or cellulose acetate electrophoresis was performed on the CSF of one thousand patients. Sepharose 0-7 colony stimulating factor 2 Homo sapiens 70-73 7236776-1 1980 Covalent binding of the pyridoxal phosphate-dependent lyase--cystathionine-beta-synthase from chicken liver--by CNBr-activated Sepharose 4B and 6B resulted in catalytically active preparations of immobilized enzyme. Sepharose 127-136 cystathionine beta-synthase Gallus gallus 61-88 6170512-2 1981 The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast, reacts immunologically with interferon-beta separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and absorbs interferon-beta immunologically when bound to CNBr-activated Sepharose. Sepharose 355-364 interferon beta 1 Homo sapiens 154-169 6170512-2 1981 The antibody inhibits the antiviral activity of human fibroblast interferon in an antiviral assay using human FS4 fibroblast, reacts immunologically with interferon-beta separated by sodium dodecylsulfate/polyacrylamide gel electrophoresis and subsequent transfer to nitrocellulose and absorbs interferon-beta immunologically when bound to CNBr-activated Sepharose. Sepharose 355-364 interferon beta 1 Homo sapiens 294-309 6106652-4 1980 DMS 53 SRIF-LI was quantitatively retained on an immunoaffinity column of sheep anti-SRIF-Sepharose 4B under neutral conditions and could be eluted with 2 N acetic acid. Sepharose 90-102 somatostatin Mus musculus 7-11 7214614-0 1980 Interactions of Sepharose-bound neurophysin I and II with oxytocin and vasopressin. Sepharose 16-25 oxytocin/neurophysin I prepropeptide Homo sapiens 32-45 7214614-0 1980 Interactions of Sepharose-bound neurophysin I and II with oxytocin and vasopressin. Sepharose 16-25 arginine vasopressin Homo sapiens 71-82 6106652-4 1980 DMS 53 SRIF-LI was quantitatively retained on an immunoaffinity column of sheep anti-SRIF-Sepharose 4B under neutral conditions and could be eluted with 2 N acetic acid. Sepharose 90-102 somatostatin Mus musculus 85-89 6253450-5 1980 Fractionation of the irradiated mitochondria in the presence of detergents and salts followed by chromatography on an agarose Bio-Gel-A-5m showed that the labeled cytochrome c was bound covalently to succinate-cytochrome c reductase. Sepharose 118-125 cytochrome c, somatic Equus caballus 163-175 6448862-12 1980 The presence of Ca++-dependent calmodulin-binding sites on 14S and 30S dyneins was demonstrated by the Ca++-dependent retention of the dyneins on a calmodulin-Sepharose-4B column. Sepharose 159-168 calmodulin Bos taurus 31-41 6448862-12 1980 The presence of Ca++-dependent calmodulin-binding sites on 14S and 30S dyneins was demonstrated by the Ca++-dependent retention of the dyneins on a calmodulin-Sepharose-4B column. Sepharose 159-168 calmodulin Bos taurus 148-158 6776753-2 1980 To obtain the desired grade of desialylation and to avoid the presence of the enzyme in the modified hormone, neuraminidase coupled to Sepharose 4B was used. Sepharose 135-147 neuraminidase 1 Homo sapiens 110-123 7437461-1 1980 Three forms of human pituitary prolactin, separable at alkaline pH in a highly purified preparation, were isolated by means of column electrophoresis in agarose suspension. Sepharose 153-160 prolactin Homo sapiens 31-40 7459687-4 1980 Reaction of the individual glycoproteins, partially purified by affinity chromatography on concanavalin A-agarose followed by polyacrylamide gel electrophoresis, showed that GP 130 contained the most receptor sites for concanavalin A per unit of protein followed by GP 180 and GP 110. Sepharose 106-113 interleukin 6 cytokine family signal transducer Rattus norvegicus 174-180 7425147-1 1980 A rapid radioimmunoassay for serum myoglobin concentration has been developed, capitalizing on a technique for rapid separation of antibody-bound antigen from free antigen using Sepharose-bound sheep anti-rabbit gamma-globulin. Sepharose 178-187 myoglobin Homo sapiens 35-44 7459687-7 1980 Chromatography of 125I-labelled concanavalin A positive glycoproteins on wheat germ Sepharose resolved GP 110 and GP 180 into wheat germ positive and negative components indicating the presence of some structural heterogeneity within these molecular weight classes. Sepharose 84-93 ADRM1 26S proteasome ubiquitin receptor Rattus norvegicus 103-109 6780343-13 1980 This result was supported by the finding that selective proteolysis of either the alpha-subunit, or the alpha and beta subunits, decreased or abolished the ability of phosphorylase kinase to bind to calmodulin-Sepharose. Sepharose 210-219 calmodulin Oryctolagus cuniculus 199-209 6158545-5 1980 J774 FcR was judged to be a glycoprotein based on the sensitivity of its isoelectric point to neuraminidase digestion, its labeling with galactose oxidase/NaB[3H4], and its binding to concanavalin A-Sepharose. Sepharose 199-208 Fc receptor Mus musculus 5-8 6932401-6 1980 Agarose gel electrophoresis of hnRNP particle-derived RNA denatured in glyoxal followed by "Northern" transfer to diazobenzyloxymethyl paper and hybridization with 32P-labeled cloned mouse beta-globin DNA reveals the presence in hnRNP of two size classes of beta-globin gene transcripts, the larger of which corresponds to the pre-spliced 15S beta-globin mRNA precursor previously identified in whole nuclear RNA, and the smaller of which corresponds to completely processed 9S beta-globin mRNA. Sepharose 0-7 heterogeneous nuclear ribonucleoprotein H2 Mus musculus 31-36 6932401-6 1980 Agarose gel electrophoresis of hnRNP particle-derived RNA denatured in glyoxal followed by "Northern" transfer to diazobenzyloxymethyl paper and hybridization with 32P-labeled cloned mouse beta-globin DNA reveals the presence in hnRNP of two size classes of beta-globin gene transcripts, the larger of which corresponds to the pre-spliced 15S beta-globin mRNA precursor previously identified in whole nuclear RNA, and the smaller of which corresponds to completely processed 9S beta-globin mRNA. Sepharose 0-7 heterogeneous nuclear ribonucleoprotein H2 Mus musculus 229-234 7452299-2 1980 Solubilized DBH was adsorbed from crude extracts on Concanavalin A-Sepharose (Con A-Sepharose), resulting in enrichment of the enzyme as well as removal of endogenous catecholamines and inhibitory substances. Sepharose 67-76 dopamine beta-hydroxylase Bos taurus 12-15 7452299-3 1980 The enzymatic assay was carried out with DBH still adsorbed to Con A-Sepharose. Sepharose 69-78 dopamine beta-hydroxylase Bos taurus 41-44 7452299-4 1980 The adsorption of the DBH to Con A-Sepharose offers three advantages over previous assay procedures. Sepharose 35-44 dopamine beta-hydroxylase Bos taurus 22-25 6934513-6 1980 When the heat-stable calcium-binding protein, calmodulin, was removed from synaptosomal cytosol by affinity chromatography on fluphenazine-Sepharose, calcium-stimulated protein phosphorylation was abolished. Sepharose 139-148 calmodulin 1 Homo sapiens 46-56 6934514-1 1980 Vertebrate and invertebrate L-lactate dehydrogenases (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) are effectively bound to oxamate-diaminohexyl-Sepharose, whereas several D-lactate dehydrogenases (D-lactate:NAD+ oxidoreductase, EC 1.1.1.28) do not bind to the same Sepharose. Sepharose 144-153 thioredoxin reductase 1 Homo sapiens 69-83 6934514-1 1980 Vertebrate and invertebrate L-lactate dehydrogenases (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) are effectively bound to oxamate-diaminohexyl-Sepharose, whereas several D-lactate dehydrogenases (D-lactate:NAD+ oxidoreductase, EC 1.1.1.28) do not bind to the same Sepharose. Sepharose 144-153 thioredoxin reductase 1 Homo sapiens 212-226 6934514-1 1980 Vertebrate and invertebrate L-lactate dehydrogenases (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) are effectively bound to oxamate-diaminohexyl-Sepharose, whereas several D-lactate dehydrogenases (D-lactate:NAD+ oxidoreductase, EC 1.1.1.28) do not bind to the same Sepharose. Sepharose 265-274 thioredoxin reductase 1 Homo sapiens 69-83 6934514-1 1980 Vertebrate and invertebrate L-lactate dehydrogenases (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) are effectively bound to oxamate-diaminohexyl-Sepharose, whereas several D-lactate dehydrogenases (D-lactate:NAD+ oxidoreductase, EC 1.1.1.28) do not bind to the same Sepharose. Sepharose 265-274 thioredoxin reductase 1 Homo sapiens 212-226 7209874-0 1980 Preparation and partial characterization of human plasma depleted of antithrombin-III by heparin-sepharose affinity chromatography. Sepharose 97-106 serpin family C member 1 Homo sapiens 69-85 7000074-0 1980 Purification of rat renal renin from crude kidney extracts by diaminohexamethylene-sepharose chromatography. Sepharose 83-92 renin Rattus norvegicus 26-31 6251060-1 1980 The iso-1-cytochrome c mRNA has been identified by hybridization of a 32P probe prepared from a plasmid containing the iso-1-cytochrome c gene to RNA size-fractionated on agarose gels and transferred to paper. Sepharose 171-178 threonine ammonia-lyase ILV1 Saccharomyces cerevisiae S288C 4-9 7191376-0 1980 The implantation of sepharose beads in mouse livers as an aid in the study of hepatic schistosomal fibrosis. Sepharose 20-29 activation-induced cytidine deaminase Mus musculus 58-61 6449829-3 1980 This substance, designated "plasmin", was separated from plasmin and kallikrein in a three-step procedure using columns of lysine-Sepharose, DEAE-Sephadex A-50, and arginine-Sepharose. Sepharose 130-139 plasminogen Homo sapiens 28-35 6449829-3 1980 This substance, designated "plasmin", was separated from plasmin and kallikrein in a three-step procedure using columns of lysine-Sepharose, DEAE-Sephadex A-50, and arginine-Sepharose. Sepharose 174-183 plasminogen Homo sapiens 28-35 6449829-7 1980 It is suggested that the reduced extent of activation of factor XII observed in plasma from rats injected intravenously with dextran, or rat plasma that has been passed through a column with lysine-Sepharose, is due to the loss of functional HMWK caused by plasmin activated in vivo or on the column. Sepharose 198-207 coagulation factor XII Rattus norvegicus 57-67 6452123-12 1980 Partial depolymerization of heparin led to a decrease in ability to displace lipoprotein lipase from heparin-Sepharose; however, even fragments of less than decasaccharide size showed definite enzyme-releasing activity. Sepharose 109-118 lipoprotein lipase Rattus norvegicus 77-95 6254766-3 1980 It was used to demonstrate that actin can be released from DNAse-I-agarose by 35--40% formamide. Sepharose 67-74 actin Oryctolagus cuniculus 32-37 6254766-3 1980 It was used to demonstrate that actin can be released from DNAse-I-agarose by 35--40% formamide. Sepharose 67-74 deoxyribonuclease-1 Oryctolagus cuniculus 59-66 6252206-0 1980 Affinity chromatography of porcine pancreas deoxyribonuclease I on DNA-binding sepharose under non-digestive conditions, using its substrate-binding site. Sepharose 79-88 deoxyribonuclease 1 Homo sapiens 44-63 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 195-202 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 78-99 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 195-202 lactalbumin, alpha Rattus norvegicus 497-514 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 195-202 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 527-548 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 221-230 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 78-99 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 221-230 lactalbumin, alpha Rattus norvegicus 497-514 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 221-230 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 527-548 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 303-312 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 78-99 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 303-312 lactalbumin, alpha Rattus norvegicus 497-514 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 303-312 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 527-548 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 303-312 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 78-99 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 303-312 lactalbumin, alpha Rattus norvegicus 497-514 24233880-1 1981 A new conjugate for the affinity chromatography of UDP-galactose:glycoprotein galactosyltransferase has been synthesized by coupling hen ovomucoid, a ligand similar to the acceptor substrate, to agarose.The hen ovomucoid-Sepharose conjugate binds galactosyl transferase more tightly that other acceptor-Sepharose conjugates.The new adsorbent gives comparable yields and purifications with those obtained by ligands similar to the nucleotide moiety of the substrate and to the "specifier" protein, alpha-lactalbumin.The soluble galactosyltransferase from rat ventral prostate is effectively removed from the high speed supernatant by an ovomucoid-Sepharose column. Sepharose 303-312 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 527-548 6932503-2 1980 Recently, we have found that a proline-rich and basic glycoprotein (MGP) from human parotid saliva, which is successfully purified by Concanavalin A-Sepharose affinity chromatography, binds to some oral streptococci such as S. mitis and S. sanguis. Sepharose 149-158 matrix Gla protein Homo sapiens 68-71 7001472-1 1980 Extranuclear estrogen receptor protein (estrophilin) of MCF-7 human breast cancer cells was purified by passage of the cytosol fraction of a cell homogenate through an affinity column of estradiol linked to Sepharose by a substituted di-n-propyl sulfide bridge in the 17 alpha position. Sepharose 207-216 estrogen receptor 1 Homo sapiens 13-30 6773561-3 1980 After denaturation, three fragments (called F1, F2, and F3) were isolated by agarose gel filtration and carboxymethylcellulose chromatography. Sepharose 77-84 coagulation factor II, thrombin Gallus gallus 48-58 7407076-4 1980 The soluble transferrin receptor has been purified approximately 1500-fold by affinity chromatography using transferrin coupled to Sepharose. Sepharose 131-140 transferrin receptor Rattus norvegicus 12-32 7407076-4 1980 The soluble transferrin receptor has been purified approximately 1500-fold by affinity chromatography using transferrin coupled to Sepharose. Sepharose 131-140 transferrin Rattus norvegicus 12-23 7407076-5 1980 Experiments demonstrated that the receptor can be adsorbed to a transferrin-Sepharose gel and be eluted specifically with transferrin. Sepharose 76-85 transferrin Rattus norvegicus 64-75 7407076-5 1980 Experiments demonstrated that the receptor can be adsorbed to a transferrin-Sepharose gel and be eluted specifically with transferrin. Sepharose 76-85 transferrin Rattus norvegicus 122-133 7407216-6 1980 GD3 synthase appeared to be a glycoprotein since the activity bound to concanavalin A-Sepharose and was eluted, with increased specific activity, by alpha-methyl mannoside. Sepharose 86-95 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 Rattus norvegicus 0-12 7407251-1 1980 Collagen-fibronectin complexes, formed by binding of fibronectin to gelatin or collagen insolubilized on Sepharose, were found to bind 20-40% of radioactivity in [35S]heparin. Sepharose 105-114 fibronectin 1 Homo sapiens 9-20 7407251-1 1980 Collagen-fibronectin complexes, formed by binding of fibronectin to gelatin or collagen insolubilized on Sepharose, were found to bind 20-40% of radioactivity in [35S]heparin. Sepharose 105-114 fibronectin 1 Homo sapiens 53-64 7407251-2 1980 Fibronectin attached directly to Sepharose also bound [35S]heparin, while gelatin-Sepharose without fibronectin did not. Sepharose 33-42 fibronectin 1 Homo sapiens 0-11 6250577-11 1980 Furthermore, binding of CaM to troponin I-Sepharose was inhibited by these ligands. Sepharose 42-51 calmodulin 1 Homo sapiens 24-27 6159774-3 1980 Subnormal levels of factor XII apparently present in plasma from one DR, and after affinity chromatography on a lysine-Sepharose column also in plasma from another DR, were normalized by addition of plasma deficient in factor XII or by addition of purified HMWK. Sepharose 119-128 coagulation factor XII Rattus norvegicus 20-30 6159774-6 1980 Previous experiments with rat plasma demonstrated that plasmin and also a plasmin-like factor without affinity for lysine-Sepharose were able to destroy the capacity of HMWK to function as a cofactor in the surface-dependent activation of factor XII, without a corresponding release of kinin. Sepharose 122-131 kininogen 1 Homo sapiens 169-173 6159774-6 1980 Previous experiments with rat plasma demonstrated that plasmin and also a plasmin-like factor without affinity for lysine-Sepharose were able to destroy the capacity of HMWK to function as a cofactor in the surface-dependent activation of factor XII, without a corresponding release of kinin. Sepharose 122-131 coagulation factor XII Rattus norvegicus 239-249 6249394-1 1980 Calmodulin coupled to Sepharose has provided a rapid and sensitive means of isolating a cyclic nucleotide phosphodiesterase activity which is stimulated by the calmodulin-Ca2+ complex, from rat parotid gland. Sepharose 22-31 calmodulin 1 Rattus norvegicus 160-170 6249394-3 1980 However, it was possible to readily demonstrate the presence of a cyclic nucleotide phosphodiesterase activity regulated by calmodulin if the extracts were first purified by batch ion-exchange chromatography over DEAE-cellulose followed by affinity chromatography with calmodulin coupled to Sepharose. Sepharose 291-300 calmodulin 1 Rattus norvegicus 124-134 6249394-4 1980 The batch ion-exchange chromatography step removed the major portion of free parotid calmodulin which could compete with calmodulin-coupled Sepharose for the proteins regulated by calmodulin. Sepharose 140-149 calmodulin 1 Rattus norvegicus 85-95 6249394-4 1980 The batch ion-exchange chromatography step removed the major portion of free parotid calmodulin which could compete with calmodulin-coupled Sepharose for the proteins regulated by calmodulin. Sepharose 140-149 calmodulin 1 Rattus norvegicus 121-131 6249394-4 1980 The batch ion-exchange chromatography step removed the major portion of free parotid calmodulin which could compete with calmodulin-coupled Sepharose for the proteins regulated by calmodulin. Sepharose 140-149 calmodulin 1 Rattus norvegicus 121-131 6249394-5 1980 Thus, by employing an initial chromatography step over DEAE-cellulose to separate phosphodiesterase activity from calmodulin, it was possible to increase the recovery of calmodulin-sensitive phosphodiesterase after affinity chromatrography with calmodulin coupled to Sepharose. Sepharose 267-276 calmodulin 1 Rattus norvegicus 114-124 6249394-5 1980 Thus, by employing an initial chromatography step over DEAE-cellulose to separate phosphodiesterase activity from calmodulin, it was possible to increase the recovery of calmodulin-sensitive phosphodiesterase after affinity chromatrography with calmodulin coupled to Sepharose. Sepharose 267-276 calmodulin 1 Rattus norvegicus 170-180 6249394-5 1980 Thus, by employing an initial chromatography step over DEAE-cellulose to separate phosphodiesterase activity from calmodulin, it was possible to increase the recovery of calmodulin-sensitive phosphodiesterase after affinity chromatrography with calmodulin coupled to Sepharose. Sepharose 267-276 calmodulin 1 Rattus norvegicus 170-180 6968556-1 1980 Selective serum IgA deficiency (defined as a serum IgA level less than 50 mg/l) was detected in 14 of 6,191 Australian blood donors screened by double diffusion analysis of serum specimens in agarose. Sepharose 192-199 CD79a molecule Homo sapiens 16-19 6250844-2 1980 Sepharose chromatographies of ([3H]prostaglandin F2 alpha)-receptor complex gave a Stokes" radius of 630 nm. Sepharose 0-9 prostaglandin F receptor Bos taurus 35-67 6257503-3 1980 A significant amount of the "big" IR-ACTH applied bound to the concanavalin A-agarose column and was eluted with 0.2 M alpha-methyl-D-mannopyranoside, indicating the glycoprotein content of "big" IR-ACTH fractions. Sepharose 78-85 proopiomelanocortin Homo sapiens 37-41 6772699-1 1980 The galactosyltransferase has been purified from human parotid saliva by ammonium sulfate precipitation (25-70% saturation), followed by repeated affinity chromatography on Sepharose-alpha-lactalbumin. Sepharose 173-182 lactalbumin alpha Homo sapiens 183-200 6776155-2 1980 We describe a method utilising immunofixation after agarose or cellulose acetate membrane electrophoresis which allows large batches of samples to be screened rapidly for the presence of activation products of C3 and factor B. Sepharose 52-59 complement C3 Homo sapiens 210-225 6967934-6 1980 Single colonies removed from agarose undergo extensive proliferation (20-30 generations) in liquid culture with the aid of T cell growth factor provided by conditioned medium. Sepharose 29-36 interleukin 2 Homo sapiens 123-143 6107909-4 1980 Calmodulin-free tryptophan hydroxylase prepared by affinity chromatography on fluphenazine-Sepharose is not activated by ATP-Mg2+ whereas addition of calmodulin to calmodulin-free enzyme restores the responsiveness of the hydroxylase to ATP-MG2+ only in the presence of Ca2+. Sepharose 91-100 calmodulin 1 Homo sapiens 0-10 6771264-7 1980 The affinity for heparin was utilized for the isolation of heparin-binding domains of fibronectin on heparin-agarose affinity columns. Sepharose 109-116 fibronectin 1 Gallus gallus 86-97 6104548-4 1980 The affinity of serum-gamma-glutamyltranspeptidase to concanavalin A-Sepharose was compared with those of the Triton-solubilized and papain-solubilized enzymes. Sepharose 69-78 inactive glutathione hydrolase 2 Homo sapiens 22-50 6933437-3 1980 The transferase was purified approximately 2000-fold by fractionation on a bovine alpha-lactalbumin-Sepharose column; the active material bound quantitatively to the gel and was eluted by removal of divalent cation from the wash buffer. Sepharose 100-109 lactalbumin alpha Bos taurus 82-99 7448618-1 1980 Lecithin:cholesterol acyltransferase (LCAT) has been purified from human plasma by sequential preparative ultracentrifugation, ion-exchange chromatography on DEAE-Sephacel, and affinity chromatography on HDL-Sepharose and on wheat germ agglutinin-Sepharose. Sepharose 208-217 lecithin-cholesterol acyltransferase Homo sapiens 0-36 7448618-1 1980 Lecithin:cholesterol acyltransferase (LCAT) has been purified from human plasma by sequential preparative ultracentrifugation, ion-exchange chromatography on DEAE-Sephacel, and affinity chromatography on HDL-Sepharose and on wheat germ agglutinin-Sepharose. Sepharose 208-217 lecithin-cholesterol acyltransferase Homo sapiens 38-42 7448618-1 1980 Lecithin:cholesterol acyltransferase (LCAT) has been purified from human plasma by sequential preparative ultracentrifugation, ion-exchange chromatography on DEAE-Sephacel, and affinity chromatography on HDL-Sepharose and on wheat germ agglutinin-Sepharose. Sepharose 247-256 lecithin-cholesterol acyltransferase Homo sapiens 0-36 7448618-1 1980 Lecithin:cholesterol acyltransferase (LCAT) has been purified from human plasma by sequential preparative ultracentrifugation, ion-exchange chromatography on DEAE-Sephacel, and affinity chromatography on HDL-Sepharose and on wheat germ agglutinin-Sepharose. Sepharose 247-256 lecithin-cholesterol acyltransferase Homo sapiens 38-42 6104515-3 1980 Guanylate cyclase eluted in the void volume when detergent-solubilized rod outer segments were subjected to exclusion chromatography on Sepharose 4B. Sepharose 136-145 guanylate cyclase Bos taurus 0-17 6247353-1 1980 Calmodulin-dependent cyclic nucleotide phosphodiesterase was purified from bovine brain to apparent homogeneity by a new procedure involving DEAE-cellulose, Affi-Gel blue, calmodulin-Sepharose 4B, and Sephadex G-200 column chromatographies. Sepharose 183-192 calmodulin Bos taurus 0-10 7004490-1 1980 Under conditions which cause dissociation of soluble tetrameric glyceraldehyde-3-phosphate dehydrogenase (D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12) into inactive dimers, immobilized apoenzymes from yeast and rat skeletal muscle coupled to CnBr-activated Sepharose via one subunit retain 50% of matrix-bound protein with unaltered specific activity. Sepharose 293-302 glyceraldehyde-3-phosphate dehydrogenase Rattus norvegicus 64-104 7448199-2 1980 Amastatin, a specific inhibitor of aminopeptidase A (L-alpha-aspartyl(L-alpha-glutamyl)-peptide hydrolase, EC 3.4.11.7), was linked to an agarose matrix and by this affinity chromatography aminopeptidase A of pig kidneys was purified as a single protein shown by acrylamide gel electrophoresis. Sepharose 138-145 glutamyl aminopeptidase Sus scrofa 35-51 7448199-2 1980 Amastatin, a specific inhibitor of aminopeptidase A (L-alpha-aspartyl(L-alpha-glutamyl)-peptide hydrolase, EC 3.4.11.7), was linked to an agarose matrix and by this affinity chromatography aminopeptidase A of pig kidneys was purified as a single protein shown by acrylamide gel electrophoresis. Sepharose 138-145 glutamyl aminopeptidase Sus scrofa 189-205 7455972-2 1980 We obtained different antithrombin III patterns in the plasma of the affected members of the two families with the modified two dimensional immunoelectrophoresis method (heparin in agarose). Sepharose 181-188 serpin family C member 1 Homo sapiens 22-38 7455972-4 1980 In the other, the antithrombin III displayed a decreased electrophoretic mobility in the heparinized agarose gel. Sepharose 101-108 serpin family C member 1 Homo sapiens 18-34 6774174-0 1980 Different chromatographic behaviours on blue sepharose CL-6B of free and estradiol complexed forms of the estrogen receptor from DMBA-induced rat mammary tumors. Sepharose 45-54 estrogen receptor 1 Rattus norvegicus 106-123 7455972-5 1980 The relatively low affinity of this antithrombin III to heparin could be directly proved by the heparin-agarose affinity chromatography, too. Sepharose 104-111 serpin family C member 1 Homo sapiens 36-52 6104981-2 1980 The protein structure of the enzyme was defined by sodium dodecyl sulfate gel electrophoresis, which revealed a single protein band of 75000 daltons that was coincident with the profile of ATPase activity resolved by the final step of agarose-ATP chromatography or by isoelectric focusing. Sepharose 235-242 dynein axonemal heavy chain 8 Homo sapiens 189-195 6934702-0 1980 A simple method for the assay of bile salt sulfotransferase using glycolithocholate coupled to Sepharose. Sepharose 95-104 sulfotransferase family 2A member 1 Homo sapiens 33-59 6245805-4 1980 Cytological examination of the agarose colonies confirmed their SCCL origin. Sepharose 31-38 SCLC1 Homo sapiens 64-68 7398108-0 1980 Detection of Lesch-Nyhan syndrome carriers: analysis of hair roots for HPRT by agarose gel electrophoresis and autoradiography. Sepharose 79-86 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 71-75 6893050-1 1980 Affinity chromatography with actin-Sepharose conjugates of purified human fibronectin, normal human plasma, or serum-free culture fluid from human fibroblasts showed that fibronectin molecules can directly bind to actin. Sepharose 35-44 fibronectin 1 Homo sapiens 74-85 6893050-1 1980 Affinity chromatography with actin-Sepharose conjugates of purified human fibronectin, normal human plasma, or serum-free culture fluid from human fibroblasts showed that fibronectin molecules can directly bind to actin. Sepharose 35-44 fibronectin 1 Homo sapiens 171-182 6893050-2 1980 A quantitative recovery of soluble human fibronectin was accomplished by chromatography on actin immobilized on Sepharose beads. Sepharose 112-121 fibronectin 1 Homo sapiens 41-52 6893050-3 1980 Human fibronectin molecules bound to actin-Sepharose were eluted with 0.25--0.35 M potassium bromide, and these molecules competed in a species-specific radioimmunoassay for human fibronectin. Sepharose 43-52 fibronectin 1 Homo sapiens 6-17 6893050-3 1980 Human fibronectin molecules bound to actin-Sepharose were eluted with 0.25--0.35 M potassium bromide, and these molecules competed in a species-specific radioimmunoassay for human fibronectin. Sepharose 43-52 fibronectin 1 Homo sapiens 180-191 6893050-4 1980 The subunits of fibronectin isolated by actin-Sepharose chromatography comigrated in SDS polyacrylamide gel electrophoresis with those of electrophoretically homogeneous fibronectin purified by conventional procedures. Sepharose 46-55 fibronectin 1 Homo sapiens 16-27 6246947-7 1980 Sepharose-bound anti-transferrin was used to purify the 125I-labeled transferrin-membrane complexes. Sepharose 0-9 serotransferrin Oryctolagus cuniculus 21-32 6246947-7 1980 Sepharose-bound anti-transferrin was used to purify the 125I-labeled transferrin-membrane complexes. Sepharose 0-9 serotransferrin Oryctolagus cuniculus 69-80 6249270-5 1980 The binding activity for human 125I-labelled-somatotropin was adsorbed to a concanavalin-A-Sepharose column and was dissociated from the column with alpha-methyl-D-glucoside, suggesting that the binding protein may be a glycoprotein. Sepharose 91-100 growth hormone 1 Homo sapiens 45-57 6253107-4 1980 Syncytiotrophoblast cells of the normal placenta from the first half of pregnancy and trophoblastic tumours, showed staining with anti-LRF antiserum after solid phase immuno-adsorption with Sepharose-coupled BSA. Sepharose 190-199 CREB3 regulatory factor Homo sapiens 135-138 6253107-5 1980 Adjacent control sections showed no staining when prepared with anti-LRF antiserum after immunoadsorption with a Sepharose-coupled LRF-BSA conjugate. Sepharose 113-122 CREB3 regulatory factor Homo sapiens 131-134 6447502-2 1980 An inhibitor of the plasma proteinase plasmin (EC 3.4.21.7) was partially purified from washed and lysed human blood platelets by (NH4)2SO4 fractionation and affinity chromatrography on Sepharose-linked purified plasminogen. Sepharose 186-195 endogenous retrovirus group K member 18 Homo sapiens 27-37 7397182-0 1980 Isolation of the newly synthesized DNA of HeLa cells containing beta-2"-deoxy-6-thioguanylate on an organomercurial agarose column. Sepharose 116-123 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 64-70 7448009-3 1980 Soluble and insoluble glycoproteins from adult bovine cerebellum have been separated by affinity chromatography on ConA-Sepharose and analyzed by polyacrylamide gel electrophoresis I2% in presence of SDS. Sepharose 120-129 CONA Bos taurus 115-119 6772690-7 1980 The thrombin agarose (total antithrombin) gel diffusion technique correlated less well with the chromogenic (r = 0.65; P less than 0.01) and Mancini immunoassay (r = 0.45; P less than 0.01) methods. Sepharose 13-20 coagulation factor II, thrombin Homo sapiens 4-12 6772690-7 1980 The thrombin agarose (total antithrombin) gel diffusion technique correlated less well with the chromogenic (r = 0.65; P less than 0.01) and Mancini immunoassay (r = 0.45; P less than 0.01) methods. Sepharose 13-20 serpin family C member 1 Homo sapiens 28-40 6772690-9 1980 The thrombin agarose diffusion method, though not specific for antithrombin III, is a cheap and simple method to perform. Sepharose 13-20 coagulation factor II, thrombin Homo sapiens 4-12 7373717-2 1980 The EHV-3 dTK was purified from KyED cytosol dTK by affinity chromatography on deoxythymidine-Sepharose and characterized with respect to its electrophoretic mobility, molecular weight, substrate specificity, phosphate donor specificity, and immunological specificity. Sepharose 94-103 Tachykinin Drosophila melanogaster 10-13 7370750-3 1980 Rat cerebral CCK was purified by immuno-affinity adsorption to the IgG fraction of CCK antisera conjugated to Sepharose beads, and by gel filtration and ion exchange chromatography. Sepharose 110-119 cholecystokinin Rattus norvegicus 13-16 6447502-2 1980 An inhibitor of the plasma proteinase plasmin (EC 3.4.21.7) was partially purified from washed and lysed human blood platelets by (NH4)2SO4 fractionation and affinity chromatrography on Sepharose-linked purified plasminogen. Sepharose 186-195 plasminogen Homo sapiens 38-45 6447502-8 1980 The inhibitor increased the mobility in agarose-gel electrophoresis of purified activator-free plasmin or 125I-labelled plasmin, as demonstrated by crossed-immunoelectrophoresis against specific immunoglobulins against plasminogen or by radioautography. Sepharose 40-47 plasminogen Homo sapiens 95-102 6447502-8 1980 The inhibitor increased the mobility in agarose-gel electrophoresis of purified activator-free plasmin or 125I-labelled plasmin, as demonstrated by crossed-immunoelectrophoresis against specific immunoglobulins against plasminogen or by radioautography. Sepharose 40-47 plasminogen Homo sapiens 120-127 18770251-5 1980 Investigations into the organ origin of the plasma ALP isoenzymes with agarose electrophoresis, column chromatography, L phenylalanine inhibition and heat treatment showed that the main part of ALP activity in blood plasma of both, inoculated and uninoculated birds was most likely of intestinal origin. Sepharose 71-78 alkaline phosphatase, placental Homo sapiens 51-54 18770251-5 1980 Investigations into the organ origin of the plasma ALP isoenzymes with agarose electrophoresis, column chromatography, L phenylalanine inhibition and heat treatment showed that the main part of ALP activity in blood plasma of both, inoculated and uninoculated birds was most likely of intestinal origin. Sepharose 71-78 alkaline phosphatase, placental Homo sapiens 194-197 6766333-1 1980 The hydrophobic interaction of beta-galactosidase with Sepharose 4B substituted with 3,3"-diaminodipropylamine was studied in both batch and column experiments. Sepharose 55-67 galactosidase beta 1 Homo sapiens 31-49 6244299-1 1980 A temperature-sensitive single-stranded DNA-binding protein (SSB) has been purified from mutant Escherichia coli (ssb-1) cells by use of affinity chromatography on blue dextran-Sepharose. Sepharose 177-186 single-stranded DNA-binding protein Escherichia coli 61-64 6987270-13 1980 Agarose column chromatography of fasting plasma confirmed that the bulk of plasma apoA-IV is free, unassociated with lipoproteins. Sepharose 0-7 apolipoprotein A4 Homo sapiens 82-89 16592801-6 1980 The calmodulin synthesized in vitro has been isolated by using calcium-dependent affinity chromatography on phenothiazine-Sepharose conjugates. Sepharose 122-131 calmodulin Bos taurus 4-14 16661245-9 1980 When 5- to 8-day-old cotyledons were extracted and purified, further elution of the blue-Sepharose with KNO(3), subsequent to the NADPH elution, yielded an NR fraction most active with NADH. Sepharose 89-98 inducible nitrate reductase [NADH] 1 Glycine max 156-158 6768398-2 1980 Incubation with intact human plasma high density lipoprotein or with lipid-free apolipoprotein A-I, however, disrupted the lipid-rich complex almost completely and most of the enzyme activity eluted from a 2% agarose column at about Ve = 2.3 x Vo. Sepharose 209-216 apolipoprotein A1 Homo sapiens 80-98 7358677-1 1980 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) from beef brain has been purified 3100-fold to apparent homogeneity using a purification procedure based on GMP-Sepharose affinity chromatography. Sepharose 172-181 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-46 6153908-1 1980 Mouse peritoneal exudate macrophage elastase can be significantly purified with 60% recovery of the starting activity by affinity chromatography against SDS-treated alpha-elastin covalently linked to agarose beads. Sepharose 200-207 matrix metallopeptidase 12 Mus musculus 25-44 7362830-1 1980 Antithrombin III was purified from rat plasma in 70% yield by salting out with (NH4)2SO4, affinity chromatography on heparin-Sepharose 4B, and ion-exchange chromatography on DE-52. Sepharose 125-134 serpin family C member 1 Rattus norvegicus 0-16 7380940-0 1980 Simple method for the purification of C1q, a subcomponent of the first component of complement by affinity chromatography using IgG-Sepharose. Sepharose 132-141 complement C1q A chain Homo sapiens 38-41 6153944-3 1980 Here we report the case of an elderly man with massive benign prostatic hyperplasia but no clinical or pathological evidence of prostatic adenocarcinoma, whose serum CK-BB activity was found by agarose gel electrophoresis to be 1 U/L (normal: 0%), 10% of his total CK activity. Sepharose 194-201 creatine kinase B Homo sapiens 166-171 7390612-5 1980 The initial procedure in MAF characterization has been chromatography on Sepharose 6B-C1 column with the denaturating solvent, 6 M guanidine HCl, pH 8.0, in order to reduce the copurification of MAF activity with serum proteins. Sepharose 73-82 MAF bZIP transcription factor Homo sapiens 25-28 6444419-4 1980 The anticoagulant was isolated by PF4-Sepharose affinity chromatography and analyzed in terms of its molecular weight, uronic acid, and amino acid composition. Sepharose 38-47 platelet factor 4 Homo sapiens 34-37 6243635-1 1980 Flavokinase (ATP:riboflavin 5"-phosphotransferase, EC 2.7.1.26) has been purified to apparent homogeneity from rat liver by affinity chromatography using flavinyl agarose beads (agarose-OCH2CONH(CH2)2NHCO(CH2)/N10-7,8-dimethylisoalloxazine). Sepharose 163-170 riboflavin kinase Homo sapiens 0-11 6991515-1 1980 Pure dipeptidyl peptidase IV (X-prolyl dipeptidyl aminopeptidase), which did not contain aminopeptidase activity at all, was rapidly prepared from the human submaxillary gland by chromatography with concanavalin A-Sepharose and Gly-Pro-NH-(CH2)6-NH-Sepharose. Sepharose 214-223 dipeptidyl peptidase 4 Homo sapiens 5-28 7357745-1 1980 A solid-phase immunoadsorbent assay for serum prostatic acid phosphatase (PAP) measurement has been developed as modified from our previously reported immunofluoroassay, utilizing the specific anti-PAP antibodies conjugated to CNBr-activated Sepharose 4B. Sepharose 242-251 acid phosphatase 3 Homo sapiens 46-72 7357745-2 1980 The serum prostatic acid phosphatase was bound, and separated from other acid phosphatases and serum proteins, by the solid-phase anti-PAP IgG Sepharose 4B. Sepharose 143-152 acid phosphatase 3 Homo sapiens 10-36 7356664-7 1980 The molecular weight of prolactin mRNA determined by electrophoresis on agarose gels containing 10 mM mercury hydroxide was 350,000. Sepharose 72-79 prolactin Homo sapiens 24-33 7356933-1 1980 Factor VIII has been purified approximately 300000-fold from bovine plasma by ammonium sulfate fractionation, glycine precipitation, DEAE-Sephadex column chromatography, sulfate--Sepharose column chromatography, Sephadex G-200 gel filtration, and factor X--Sepharose column chromatography. Sepharose 179-188 coagulation factor VIII Bos taurus 0-11 7356933-1 1980 Factor VIII has been purified approximately 300000-fold from bovine plasma by ammonium sulfate fractionation, glycine precipitation, DEAE-Sephadex column chromatography, sulfate--Sepharose column chromatography, Sephadex G-200 gel filtration, and factor X--Sepharose column chromatography. Sepharose 257-266 coagulation factor VIII Bos taurus 0-11 6155905-1 1980 Two electrophoretic variants of phosphoglucose isomerase (PGI) were purified from whole body extracts of DBA/2J and C57BL/6J mice by a substrate-affinity elution from an 8-(6-aminohexyl)amino-ATP-Sepharose column followed by preparative isoelectric focusing. Sepharose 196-205 glucose-6-phosphate isomerase 1 Mus musculus 32-56 6155905-1 1980 Two electrophoretic variants of phosphoglucose isomerase (PGI) were purified from whole body extracts of DBA/2J and C57BL/6J mice by a substrate-affinity elution from an 8-(6-aminohexyl)amino-ATP-Sepharose column followed by preparative isoelectric focusing. Sepharose 196-205 glucose-6-phosphate isomerase 1 Mus musculus 58-61 6986392-4 1980 The tumor PSbetaG was adsorbed to concanavalin A-Sepharose and eluted with alpha-D-methyl-glucoside in a manner similar to purified PSbetaG. Sepharose 49-58 pregnancy specific beta-1-glycoprotein 10, pseudogene Homo sapiens 10-17 7352259-1 1980 The purification of human fibroblast interferon has been simplified to a two-step procedure consisting of affinity chromatography on Blue Sepharose and sodium dodecyl sulfate polyacrlamide gel electrophoresis. Sepharose 138-147 interferon beta 1 Homo sapiens 26-47 6159869-1 1980 A competitive Sepharose bead immunofluorescence assay is described which allows the quantitative detection of human alpha-fetoprotein. Sepharose 14-23 alpha fetoprotein Homo sapiens 116-133 7447641-1 1980 Proteinase inhibitors adsorbed from human serum on DEAE Sephadex A 25 0.25 or 0.3 mol/l NaCl were purified by affinity chromatography on a trypsin-Sepharose 4B column, by gel filtration, and by DEAE cellulose chromatography. Sepharose 147-156 endogenous retrovirus group K member 25 Homo sapiens 0-10 6908522-1 1980 A serine protease active on insoluble elastin at neutral pH has been isolated from human aortic media employing a Lima-bean trypsin inhibitor - Sepharose column. Sepharose 144-153 elastin Homo sapiens 38-45 7192096-1 1980 5th Communication: Further characterization by affinity chromatography on antithrombin-sepharose and its comparison with a commercially available heparin. Sepharose 87-96 serpin family C member 1 Homo sapiens 74-86 7192096-2 1980 A highly potent heparin (Heparin Schering) was characterized in comparison to commercial heparin by affinity chromatography on antithrombin-III-sepharose. Sepharose 144-153 serpin family C member 1 Homo sapiens 127-143 7192096-4 1980 The specific biological activities per microgram uronic acid and elution pattern on antithrombin-sepharose of high affinity heparin fraction were similar for both heparin preparations. Sepharose 97-106 serpin family C member 1 Homo sapiens 84-96 7436742-1 1980 Lipoprotein lipase of bovine aortic intima has been purified to homogeneity by affinity chromatography on heparin-Sepharose. Sepharose 114-123 lipoprotein lipase Bos taurus 0-18 6769429-1 1980 alpha-Lactalbumin was purified to apparent homogeneity from mouse milk by combined use of gel filtration, chromatography on DEAE-cellulose and hydroxyapatite, and concanavalin A-Sepharose affinity chromatography. Sepharose 178-187 lactalbumin, alpha Mus musculus 0-17 6769429-6 1980 The larger form of alpha-lactalbumin, consisting of two species with different charges, was present in a small amount (less than 10%) in the milk and was isolated by its ability to interact with concanavalin A-Sepharose. Sepharose 210-219 lactalbumin, alpha Mus musculus 19-36 7417594-4 1980 Thus AT III and heparin were co-immobilized on Sepharose 4B, polyvinyl alcohol, polyhydroxy-ethyl methacrylate and silicone-coated nylon by the cyanogen bromide procedure. Sepharose 47-59 serpin family C member 1 Homo sapiens 5-11 6971130-1 1980 Urinary colony stimulating factor (CSF) from human leukemic urine is retained on propylamine-agarose hydrophobic chromatography; about 70% of total activity is eluted by the addition of 1 M NaCl with significant increase in specific activity. Sepharose 93-100 colony stimulating factor 2 Homo sapiens 35-38 7213837-1 1980 The preparation of affinity adsorbents for menadione reductase (EC 1.6.99.2), using CNBr-activated Sepharose 4B and activated CH-Sepharose 4B, is described. Sepharose 99-108 NAD(P)H quinone dehydrogenase 1 Homo sapiens 43-62 7213837-1 1980 The preparation of affinity adsorbents for menadione reductase (EC 1.6.99.2), using CNBr-activated Sepharose 4B and activated CH-Sepharose 4B, is described. Sepharose 129-138 NAD(P)H quinone dehydrogenase 1 Homo sapiens 43-62 6243087-2 1980 Antibodies cross-reactive with type C viral p30 were concentrated by means of affinity chromatography using Sepharose beads to which disrupted Simian sarcoma virus or Rauscher murine leukemia virus p30 had been coupled. Sepharose 108-117 centromere protein V Homo sapiens 44-47 7357606-2 1980 In this paper we describe large nuclear RNAs containing vitellogenin mRNA sequences as revealed by hybridization of cloned vitellogenin cDNAs to nuclear RNA separated on agarose gels. Sepharose 170-177 a1-a Xenopus laevis 56-68 7398638-1 1980 Binding of eukaryotic initiation factor 4C (eIF-4C) to a 40-S initiation complex could be detected by analysis of assay mixtures for 40-S initiation complex formation on columns of Sepharose 6B and on sucrose gradients. Sepharose 181-190 eukaryotic translation initiation factor 1A Y-linked Homo sapiens 11-42 7398638-1 1980 Binding of eukaryotic initiation factor 4C (eIF-4C) to a 40-S initiation complex could be detected by analysis of assay mixtures for 40-S initiation complex formation on columns of Sepharose 6B and on sucrose gradients. Sepharose 181-190 eukaryotic translation initiation factor 1A Y-linked Homo sapiens 44-50 6772531-4 1980 Relative amounts of fractions eluted in higher concentrations of MgCl2 were augmented with the increasing amount of IgG bound to SpA-Sepharose. Sepharose 133-142 pulmonary surfactant-associated protein A Sus scrofa 129-132 7450390-1 1980 Human pancreatic Deoxyribonuclease I (DNase I), inhibitor was partially purified from duodenal juice of healthy subjects collected in the Pancreozymin-Secretin test, by a procedure which included ammonium sulfate fractionation, DEAE cellulose fractionation, Sepharose 4B affinity chromatography, and gel filtration. Sepharose 258-267 deoxyribonuclease 1 Homo sapiens 38-45 7007160-6 1980 Agarose gel electrophoresis has revealed that plasmids of this group occur as a result of deletions in plasmid pEG1. Sepharose 0-7 mesoderm specific transcript Homo sapiens 111-115 7007160-8 1980 Agarose gel has demonstrated that some of them are deletion mutants while others are quite similar to pEG1 plasmid. Sepharose 0-7 mesoderm specific transcript Homo sapiens 102-106 6153632-3 1980 The complexes between elastase and alpha 1-antitrypsin and alpha 2-macroglobulin, respectively, migrate as alpha 2-globulin on agarose gel electrophoresis. Sepharose 127-134 serpin family A member 1 Homo sapiens 35-54 6153632-3 1980 The complexes between elastase and alpha 1-antitrypsin and alpha 2-macroglobulin, respectively, migrate as alpha 2-globulin on agarose gel electrophoresis. Sepharose 127-134 alpha-2-macroglobulin Homo sapiens 59-80 6245025-5 1980 The capability of freshly labelled hCG to bind to LH(hCG) receptor was 51% and to anti-hCG gamma-globulin Sepharose 4B, 94%. Sepharose 106-118 glycoprotein hormones, alpha polypeptide Homo sapiens 35-38 7216230-1 1980 The phenotypes of red cell glyoxalase I (GLO) were determined in two Icelandic population samples using starch-gel electrophoresis and high-voltage agarose-gel electrophoresis. Sepharose 148-155 glyoxalase I Homo sapiens 27-39 7216230-1 1980 The phenotypes of red cell glyoxalase I (GLO) were determined in two Icelandic population samples using starch-gel electrophoresis and high-voltage agarose-gel electrophoresis. Sepharose 148-155 glyoxalase I Homo sapiens 41-44 6154672-3 1980 When antigenic conversion from hepatitis B core antigen (HBcAg) to hepatitis B e antigen was achieved by treating these core particles by sonication or by passing them through an anti-HBc IgG-conjugated Sepharose 4B column, no appreciable changes were found in the above protein composition. Sepharose 203-212 keratin 88, pseudogene Homo sapiens 57-60 6448271-1 1980 An indirect assay in agarose-gel was developed to measure secretion of leukocyte migration inhibitory factor (LIF) by canine lymphocytes. Sepharose 21-28 LIF interleukin 6 family cytokine Canis lupus familiaris 71-108 6263156-0 1980 Calmodulin-activated plant microsomal Ca2+ uptake and purification of plant NAD kinase and other proteins by calmodulin-Sepharose chromatography. Sepharose 120-129 calmodulin 1 Homo sapiens 0-10 6263156-0 1980 Calmodulin-activated plant microsomal Ca2+ uptake and purification of plant NAD kinase and other proteins by calmodulin-Sepharose chromatography. Sepharose 120-129 NAD kinase Homo sapiens 76-86 6263156-0 1980 Calmodulin-activated plant microsomal Ca2+ uptake and purification of plant NAD kinase and other proteins by calmodulin-Sepharose chromatography. Sepharose 120-129 calmodulin 1 Homo sapiens 109-119 6991604-6 1980 The utility of the method is illustrated by detection of the proteinase enzymes in thermophilic actinomycete antigen preparations separated by polyacrylamide electrophoresis, crossed immunoelectrophoresis and isoelectric focusing on agarose and granulated dextran supports. Sepharose 233-240 endogenous retrovirus group K member 25 Homo sapiens 61-71 6995534-6 1980 A C5-depleted reagent (C5D) was generated from the non-adsorbed protein containing fractions obtained subsequent to the passage of freshly drawn NHS plus 10 mM EDTA through the monospecific anti-C5 Sepharose 4B column. Sepharose 198-207 complement C5 Homo sapiens 23-26 7350233-2 1980 The stimulatory capacity co-purified with the major envelope glycoprotein, gp70, and can be specifically removed from viral supernatants by absorption with anti-gp70 antibody-linked Sepharose. Sepharose 182-191 embigin Mus musculus 75-79 7350233-2 1980 The stimulatory capacity co-purified with the major envelope glycoprotein, gp70, and can be specifically removed from viral supernatants by absorption with anti-gp70 antibody-linked Sepharose. Sepharose 182-191 embigin Mus musculus 161-165 7381215-3 1980 The procedure, based on the recently reported affinity of fibronectin for gelatin, essentially consists of two steps (1) Immunization of rabbits with fibronectin purified from serum by affinity chromatography using gelatin coupled to CNBr-activated Sepharose 4B. Sepharose 249-258 fibronectin 1 Homo sapiens 58-69 6164068-1 1980 The leukocyte migration inhibitory factor (LIF) was examined in 57 test persons--27 patients with multiple sclerosis (MS) and 30 normals--with the indirect and the direct leucocyte migration inhibition test in agarose (LMIAT). Sepharose 210-217 LIF interleukin 6 family cytokine Homo sapiens 4-41 6164068-1 1980 The leukocyte migration inhibitory factor (LIF) was examined in 57 test persons--27 patients with multiple sclerosis (MS) and 30 normals--with the indirect and the direct leucocyte migration inhibition test in agarose (LMIAT). Sepharose 210-217 LIF interleukin 6 family cytokine Homo sapiens 43-46 6448271-1 1980 An indirect assay in agarose-gel was developed to measure secretion of leukocyte migration inhibitory factor (LIF) by canine lymphocytes. Sepharose 21-28 LIF interleukin 6 family cytokine Canis lupus familiaris 110-113 7374508-0 1980 Preparation and use of affinity columns with bovine milk folate-binding protein (FBP) covalently linked to Sepharose 4B. Sepharose 107-116 folate receptor alpha Bos taurus 52-79 6264234-2 1980 Affinity chromatography, showed that only 2 of the more than 25 proteins present in the total detergent solubilized oocyte membrane extract were retained on a PV-agarose column. Sepharose 162-169 Casein kinase II subunit beta Gallus gallus 159-161 7374508-0 1980 Preparation and use of affinity columns with bovine milk folate-binding protein (FBP) covalently linked to Sepharose 4B. Sepharose 107-116 folate receptor alpha Bos taurus 81-84 6155736-4 1980 In affinity chromatography on protein A Sepharose, IgG3 in the unbound and IgG1, IgG2 and IgG4 in the bound fractions were found in Immunoglobulins Schura and SRK. Sepharose 40-49 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 51-55 6255389-5 1980 The elution profile of CEA-LoVo in Concanavalin A-Sepharose B coincided with that of the First British Standard for CEA. Sepharose 50-59 CEA cell adhesion molecule 3 Homo sapiens 23-26 7360484-5 1980 The elution profile of CEA-LoVo in concanavalin A-Sepharose B coincided with that of the First British Standard for CEA. Sepharose 50-59 CEA cell adhesion molecule 3 Homo sapiens 23-26 6155666-1 1980 Human fibroblast interferon was partially purified, about 4,000-fold, on a chromatographic tandem of columns: concanavalin A-agarose leads to phenyl-agarose, to a specific activity of ca 4 x 10(7). Sepharose 125-132 interferon beta 1 Homo sapiens 6-27 6247709-0 1980 Purification of human myeloperoxidase by Concanavalin A-Sepharose affinity chromatography. Sepharose 56-65 myeloperoxidase Homo sapiens 22-37 6449119-0 1980 PGM1 subtypes determined by agarose gel isoelectrofocusing. Sepharose 28-35 phosphoglucomutase 1 Homo sapiens 0-4 6449119-1 1980 PGM1 subtypes were determined in red cell hemolysates by isoelectric focusing on agarose gel plates. Sepharose 81-88 phosphoglucomutase 1 Homo sapiens 0-4 6155736-4 1980 In affinity chromatography on protein A Sepharose, IgG3 in the unbound and IgG1, IgG2 and IgG4 in the bound fractions were found in Immunoglobulins Schura and SRK. Sepharose 40-49 ribosomal protein S6 kinase B2 Homo sapiens 159-162 540036-1 1979 Fibrinogen-fibrin-related antigen (FR antigen) was isolated from as little as 1 ml of human plasma by immuno-affinity chromatography with agarose-bound antibody to human fibrinogen. Sepharose 138-145 fibrinogen beta chain Homo sapiens 0-10 540036-1 1979 Fibrinogen-fibrin-related antigen (FR antigen) was isolated from as little as 1 ml of human plasma by immuno-affinity chromatography with agarose-bound antibody to human fibrinogen. Sepharose 138-145 fibrinogen beta chain Homo sapiens 170-180 260927-1 1979 A human colon kininogenase (kallikrein) was isolated by gel filtration on Sephacryl S-200 and affinity chromatography on Trasylolbound Sepharose, yielding a material with a specific activity of 1.3 U/mg (substrate: AcPheArgOEt). Sepharose 135-144 kallikrein related peptidase 4 Homo sapiens 8-39 500726-2 1979 The performance of the affinity chromatography on Sepharose-RNase A has been expedited through adaption of the spectrophotometric assay of ribonuclease toward 2",3"-cyclic cytidine monophosphate to determination of the inhibitor activity. Sepharose 50-59 ribonuclease A family member 1, pancreatic Homo sapiens 60-67 500831-6 1979 By using both sucrose density gradient ultracentrifugation and Sepharose 6B gel filtration, the Clq and mRF reactive material detected in some patients with necrotizing vasculitis eluted in high molecular weight fractions that were also anticomplementary. Sepharose 63-72 myelin regulatory factor Mus musculus 104-107 118968-2 1979 Human, porcine, rabbit, and rat antithrombin III have been purified by affinity chromatography using heparin-agarose. Sepharose 109-116 serpin family C member 1 Homo sapiens 32-48 528532-2 1979 The nuclease digest was successfully separated into three fractions, P1, P2, and P3, by gel filtration on a column of Sepharose 2B. Sepharose 118-127 perforin 1 Rattus norvegicus 69-83 119941-0 1979 Simultaneous determination of C1q and C3 levels in human serum by electroimmunodiffusion on agarose. Sepharose 92-99 complement C1q A chain Homo sapiens 30-33 395664-4 1979 Cross-reaction tests were done on 16 samples of onchocerciasis sera from West Africa using sonicated antigen as well as antigen-coated CNB1-activated sepharose. Sepharose 150-159 protein phosphatase 3 regulatory subunit B, alpha Homo sapiens 135-139 575443-2 1979 TeBG is adsorbed from serum samples onto a solid phase matrix of concanavalin A covalently linked to 4B Sepharose. Sepharose 104-113 sex hormone binding globulin Homo sapiens 0-4 500641-2 1979 The physical properties of the detergent-extracted NGF receptor were assessed by Sepharose 6B chromatography and sucrose density gradient ultracentrifugation studies in H2O and D2O. Sepharose 81-90 LOW QUALITY PROTEIN: tumor necrosis factor receptor superfamily member 16 Oryctolagus cuniculus 51-63 92330-0 1979 Isolation of plasma-membrane components from cultured human pancreatic cancer cells by immuno-affinity chromatography of anti-beta 2M sepharose 6MB. Sepharose 134-143 beta-2-microglobulin Homo sapiens 126-133 499687-6 1979 More than 98% of the insulin binding activity could be adsorbed to a column of concanavalin A-agarose, while about 94% of the insulin degrading activity could not be adsorbed to this column. Sepharose 94-101 insulin Cavia porcellus 21-28 521053-4 1979 The supernatants of the PMBC or the shed receptor purified on a Sepharose 4B-aggregated human IgG column blocked the EAR formation of FcRI+PMBC but had no effect on EAR information of FcRII+PMBC. Sepharose 64-76 Fc gamma receptor Ia Homo sapiens 134-138 486428-1 1979 Carboxypeptidases A and B have been isolated individually from aqueous extracts of mammalian pancreatic acetone powders by affinity chromatography on [N-(epsilon-aminocaproyl)-p-aminobenzyl]succinyl-Sepharose 4B (CABS-Sepharose). Sepharose 199-208 carboxypeptidase B1 Homo sapiens 0-25 486428-5 1979 The ease of synthesis of the ligand from its commercially available precursor, its stability, and the mild elution conditions render CABS-Sepharose an excellent affinity support for the single-column isolation of both carboxypeptidases A and B. Sepharose 138-147 carboxypeptidase B1 Homo sapiens 218-243 387059-7 1979 TNF activity could be separated from the Type I interferon of TNF serum by passage through a Cibacron blue-agarose column or by sequential salt precipitation, ion-exchange chromatography and gel filtration.2. Sepharose 107-114 tumor necrosis factor Oryctolagus cuniculus 0-3 573688-7 1979 In affinity chromatographic studies it was shown that the CoA-Sepharose preparation could bind the CoA-dependent enzymes citrate synthase and succinic thiokinase and these could be biospecifically eluted using soluble CoA. Sepharose 62-71 citrate synthase Homo sapiens 121-137 315910-2 1979 3.2.1.1), AMY2, is demonstrated in serum specimens by agarose gel electrophoresis. Sepharose 54-61 amylase alpha 2A Homo sapiens 10-14 314662-1 1979 Human and bovine factor VIII, isolated from cryoprecipitates of fresh plasma by gel filtration on Sepharose CL-2B, gave similar elution patterns and showed comparable distribution of oligomers on SDS agarose electrophoretic gels. Sepharose 200-207 coagulation factor VIII Bos taurus 17-28 116231-1 1979 DNA from newborn mice was digested with restriction endonuclease EcoRI, and a 6.6-kilobase fragment encoding immunoglobulin gamma 2b chain mRNA derived from MPC 11 myeloma was enriched about 100-fold by RPC-5 column chromatography and agarose gell electrophoresis. Sepharose 235-242 immunoglobulin heavy constant gamma 2B Mus musculus 124-132 159511-2 1979 Agarose gel filtration of these samples revealed the presence of fibrinogen derivatives both larger and smaller than the parent molecule. Sepharose 0-7 fibrinogen beta chain Homo sapiens 65-75 158901-1 1979 Ferredoxin-NADP+ reductase from the microalga Bumilleriopsis was isolated by a combination of affinity chromatography on a flavodoxin-Sepharose 4 B column and usual purification procedures. Sepharose 134-143 ferredoxin reductase Homo sapiens 0-26 572771-2 1979 Isoenzyme 2 of cinnamyl-alcohol dehydrogenase from soybean suspension cultures was purified about 3800-fold to apparent homogeneity by an improved purification procedure involving biospecific elution of the enzyme from a NADP+-agarose column. Sepharose 227-234 cinnamyl-alcohol dehydrogenase Glycine max 15-45 157580-3 1979 The plasminogen immobilized on bromocyanogen-activated sepharose and cellulose, like soluble proenzyme, has no activity of plasmin and retains the ability of being activated with streptokinase in catalytic amounts. Sepharose 55-64 plasminogen Homo sapiens 4-11 454594-6 1979 From the initial velocity rate, the time required for the phosphatidylcholine pool to double was about 12 h. Agarose-linked phospholipase A2 was used to measure the relative composition of choline- and dimethylethanolamine-phosphoglycerides in the outer surface of vesicles prepared from cells with different degrees of polar head group substitution. Sepharose 109-116 phospholipase A2 group IB Homo sapiens 124-140 109132-1 1979 Purified L-cell colony-stimulating factor (CSF) was coupled to cyanogen-bromide-activated Sepharose and used to selectively fractionate antibodies to this factor. Sepharose 90-99 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 43-46 316999-4 1979 Affinity chromatography on heparin-Sepharose separated the proteins since alpha 1-AT did not bind to the matrix. Sepharose 35-44 serpin family A member 1 Homo sapiens 74-84 286108-1 1979 A human immunoglobulin that binds carcinoembryonic antigen (CEA) was isolated from four individual normal human sera by affinity chromatography with the use of a CEA-Sepharose solid adsorbent. Sepharose 166-175 CEA cell adhesion molecule 3 Homo sapiens 34-58 286108-1 1979 A human immunoglobulin that binds carcinoembryonic antigen (CEA) was isolated from four individual normal human sera by affinity chromatography with the use of a CEA-Sepharose solid adsorbent. Sepharose 166-175 CEA cell adhesion molecule 3 Homo sapiens 60-63 286108-1 1979 A human immunoglobulin that binds carcinoembryonic antigen (CEA) was isolated from four individual normal human sera by affinity chromatography with the use of a CEA-Sepharose solid adsorbent. Sepharose 166-175 CEA cell adhesion molecule 3 Homo sapiens 162-165 6157030-1 1980 Brain cathepsin D, purified by affinity chromatography on Sepharose pepstatin columns, was incubated with synthetic peptides corresponding to the susceptible regions of the myelin basic protein encompassing the two Phe-Phe bonds. Sepharose 58-67 cathepsin D Homo sapiens 6-17 519755-1 1979 We have studied the polypeptides associated with the expression of the transforming region of the Ad5 genome by immunoprecipitating antigens (using the double antibody and protein A-Sepharose techniques) from cells infected with wild-type (wt) Ad5 or transformation-defective host range (hr) mutants and from cells transformed by Ad5. Sepharose 182-191 Alzheimer disease, familial, type 5 Homo sapiens 98-101 520313-2 1979 Spermine synthase, a propylamine transferase, which catalyses the biosynthesis of spermine from S-methyladenosylhomocystemine and spermidine has been purified to an apparent homogeneity (about 6000-fold) from bovine brain using spermine-Sepharose affinity chromatography. Sepharose 237-246 spermine synthase Bos taurus 0-17 489716-3 1979 We have separated inactive and active renin by DEAE-Sepharose column chromatography of normal human plasma at pH 7.5 and a linearly increasing sodium gradient. Sepharose 52-61 renin Homo sapiens 38-43 508775-2 1979 In rat, lipoprotein lipase and hepatic triacylglycerol lipase were separated on a heparin-Sepharose affinity chromatography. Sepharose 90-99 lipoprotein lipase Rattus norvegicus 8-26 508775-2 1979 In rat, lipoprotein lipase and hepatic triacylglycerol lipase were separated on a heparin-Sepharose affinity chromatography. Sepharose 90-99 lipase C, hepatic type Rattus norvegicus 31-61 41525-0 1979 Purification of plant calmodulin by fluphenazine-Sepharose affinity chromatography. Sepharose 49-58 calmodulin 1 Homo sapiens 22-32 226171-12 1979 Binding sites for concanavalin A and prolactin appear to be independent but closely related since (i) concanavalin A did not displace bound prolactin from its receptor, and (ii) detergent-solubilized 125I-labeled prolactin-receptor complexes bound to concanavalin A-Sepharose and were eluted by alpha-methyl-D-mannopyranoside. Sepharose 266-275 prolactin Rattus norvegicus 18-46 226171-12 1979 Binding sites for concanavalin A and prolactin appear to be independent but closely related since (i) concanavalin A did not displace bound prolactin from its receptor, and (ii) detergent-solubilized 125I-labeled prolactin-receptor complexes bound to concanavalin A-Sepharose and were eluted by alpha-methyl-D-mannopyranoside. Sepharose 266-275 prolactin Rattus norvegicus 37-46 115200-1 1979 Isolation of the corticosteroid-binding globulin CBG was achieved by 5 chromatographical steps on cortisol Sepharose, QAE-Sephadex A-50, Con A-Sepharose and hydroxylapatite. Sepharose 107-116 serpin family A member 6 Homo sapiens 17-48 115200-1 1979 Isolation of the corticosteroid-binding globulin CBG was achieved by 5 chromatographical steps on cortisol Sepharose, QAE-Sephadex A-50, Con A-Sepharose and hydroxylapatite. Sepharose 107-116 serpin family A member 6 Homo sapiens 49-52 230687-4 1979 Oligoclonal IgG was detected by agarose electrophoresis of the CSF in four of the five patients with a local synthesis of virus antibodies. Sepharose 32-39 colony stimulating factor 2 Homo sapiens 63-66 486718-1 1979 Xanthine dehydrogenase (EC 1.2.1.37) was isolated from chicken livers and immobilized by adsorption to a Sepharose derivative, prepared by reaction of n-octylamine with CNBr-activated Sepharose 4B. Sepharose 105-114 xanthine dehydrogenase Gallus gallus 0-22 486718-1 1979 Xanthine dehydrogenase (EC 1.2.1.37) was isolated from chicken livers and immobilized by adsorption to a Sepharose derivative, prepared by reaction of n-octylamine with CNBr-activated Sepharose 4B. Sepharose 184-196 xanthine dehydrogenase Gallus gallus 0-22 158524-3 1979 The plasmin-streptokinase complex binds to Sepharose-lysine and Sepharose-fibrin monomer in the same fashion as free plasmin, showing that the lysine binding sites are fully exposed in the complex. Sepharose 43-52 plasminogen Bos taurus 4-11 158524-3 1979 The plasmin-streptokinase complex binds to Sepharose-lysine and Sepharose-fibrin monomer in the same fashion as free plasmin, showing that the lysine binding sites are fully exposed in the complex. Sepharose 64-73 plasminogen Bos taurus 4-11 390656-2 1979 The technique as applied to the assay of choriomammotropin involves the use of beta-D-galactosylamine bound to agarose to separate the unbound choriomammotropin-beta-galactosidase conjugates for antibody bound conjugates. Sepharose 111-118 chorionic somatomammotropin hormone 2 Homo sapiens 41-58 390656-3 1979 When beta-galactosidase was conjugated with choriomammotropin using the N-hydroxy-succinamide ester of m-maleimidobenzoic acid the affinity of the enzyme conjugate to beta-D-galactosylamine attached to agarose diminished markedly following incubation with antibody. Sepharose 202-209 galactosidase beta 1 Homo sapiens 5-23 390656-3 1979 When beta-galactosidase was conjugated with choriomammotropin using the N-hydroxy-succinamide ester of m-maleimidobenzoic acid the affinity of the enzyme conjugate to beta-D-galactosylamine attached to agarose diminished markedly following incubation with antibody. Sepharose 202-209 chorionic somatomammotropin hormone 2 Homo sapiens 44-61 313929-1 1979 Two glycopeptides were obtained from alpha 1-protease inhibitor after extensive pronase digestion and chromatography on Bio-Gel P-10 and concanavalin A-Sepharose. Sepharose 152-161 serpin family A member 1 Homo sapiens 37-63 389498-0 1979 Participation of a histamine-Sepharose-adherent subpopulation of human mononuclear cells in the production of leucocyte migration inhibition factor (LIF) in healthy children. Sepharose 29-38 LIF interleukin 6 family cytokine Homo sapiens 149-152 503063-1 1979 Highly purified preparations of mRNA coding for ceruloplasmin (CP) ere isolated from rat liver polyribosomes using indirect immunoprecipitation of CP polysomes and poly(U)-sepharose chromatography of polysomal RNA. Sepharose 172-181 ceruloplasmin Rattus norvegicus 48-61 503063-1 1979 Highly purified preparations of mRNA coding for ceruloplasmin (CP) ere isolated from rat liver polyribosomes using indirect immunoprecipitation of CP polysomes and poly(U)-sepharose chromatography of polysomal RNA. Sepharose 172-181 ceruloplasmin Rattus norvegicus 63-65 503063-7 1979 Poly(U)-sepharose chromatography with stepwise temperature elution revealed length heterogeneity of poly(A) consisting of particular, different thermal subfractions of CP mRNA contain poly(A) consisting of 38, 90 and 165 adenylate residue. Sepharose 8-17 ceruloplasmin Rattus norvegicus 168-170 111550-3 1979 The patient"s myeloma protein, IgGCAB, and Fab fragments of IgGCAB migrated more anodally when agarose gel electrophoresis was performed in the absence of calcium ion than when electrophoresis was performed in the presence of calcium ion; 60 other myeloma proteins did not demonstrate such behavior. Sepharose 95-102 FA complementation group B Homo sapiens 43-46 115379-0 1979 Immunoglobulin class and light chain type of oligoclonal bands in CSF in multiple sclerosis determined by agarose gel electrophoresis and immunofixation. Sepharose 106-113 colony stimulating factor 2 Homo sapiens 66-69 115379-1 1979 Agarose gel electrophoresis and immunofixation of CSF and serum from 39 patients with multiple sclerosis (MS) revealed oligoclonal IgG in the CSF in all cases and oligoclonal IgA and IgM in 1 patient each. Sepharose 0-7 colony stimulating factor 2 Homo sapiens 142-145 387577-4 1979 The presence of leucocyte inhibitory factor (LIF) in the culture supernatants was tested by the agarose migration method. Sepharose 96-103 LIF interleukin 6 family cytokine Homo sapiens 16-43 387577-4 1979 The presence of leucocyte inhibitory factor (LIF) in the culture supernatants was tested by the agarose migration method. Sepharose 96-103 LIF interleukin 6 family cytokine Homo sapiens 45-48 222814-7 1979 Separation of the perfusate lipoproteins by agarose gel filtration revealed that most of the apoE secreted by the livers of hypercholesterolemic rats is found in the VLDL and LDL, whereas apoE secreted by the normal livers is distributed equally between VLDL, high density lipoproteins, and a low molecular weight fraction which corresponds to the virtually delipidated apoprotein. Sepharose 44-51 apolipoprotein E Rattus norvegicus 93-97 500121-1 1979 Serum amyloid P-component (protein SAP) was associated from mouse serum and ascitic fluids by calcium-dependent affinity chromatography on Sepharose 4B (Pharmacia) followed by gel filtration on Ultrogel AcA44 (LKB) in the presence of EDTA. Sepharose 139-151 amyloid P component, serum Mus musculus 0-25 500121-1 1979 Serum amyloid P-component (protein SAP) was associated from mouse serum and ascitic fluids by calcium-dependent affinity chromatography on Sepharose 4B (Pharmacia) followed by gel filtration on Ultrogel AcA44 (LKB) in the presence of EDTA. Sepharose 139-151 amyloid P component, serum Mus musculus 35-38 316999-5 1979 Alpha 1-AT unbound to the heparin-Sepharose was subsequently purified through con A-Sepharose affinity column. Sepharose 84-93 adrenoceptor alpha 1D Homo sapiens 0-7 465600-1 1979 Cathepsin D was isolated from the grey matter of bovine and porcine large cerebral hemispheres and purified by affinity chromatography on haemoglobin--Sepharose. Sepharose 151-160 cathepsin D Bos taurus 0-11 288074-5 1979 Sulfitolysis of highly purified material to break the inter- and intra-chain disulfide bridges and subsequent adsorption on a specific B-chain antibody covalently bound to Sepharose beads showed that the C-peptide was still connected to the B-chain. Sepharose 172-181 insulin Homo sapiens 204-213 87217-4 1979 The albumin mRNA possessed a chain length of approximately 2265 nucleotides and the AFP mRNA possesed a length of approximately 2235 nucleotides when examined under stringent denaturing conditions on agarose gels containing 10 mM methylmercury hydroxide. Sepharose 200-207 alpha-fetoprotein Rattus norvegicus 84-87 36246-0 1979 Preparation and some properties of human serum kallikrein immobilized on ARM-Sepharose. Sepharose 77-86 kallikrein related peptidase 4 Homo sapiens 47-57 36246-1 1979 A highly purified human serum kallikrein immobilized on CH-Sepharose 4-B was obtained. Sepharose 59-72 kallikrein related peptidase 4 Homo sapiens 30-40 85489-7 1979 Ovarian cyst fluid CEA activity was bound by concanavalin A-Sepharose 4B and specifically eluted with competing monosaccharide. Sepharose 60-72 CEA cell adhesion molecule 3 Homo sapiens 19-22 85489-8 1979 Lectin affinity chromatography and gel filtration chromatography over Sepharose 4B and Sephadex G-200 can be used to purify cyst fluid CEA from whole fluid or lyophilized and reconstituted samples. Sepharose 70-82 CEA cell adhesion molecule 3 Homo sapiens 135-138 287062-4 1979 The DNA polymerase alpha activity was also purified by selective precipitation with polyethylene glycol (Mr 6000) followed by agarose-concanavalin A column chromatography. Sepharose 126-133 DNA polymerase alpha 1, catalytic subunit Homo sapiens 4-24 35531-6 1979 Butanedione-modified aldehyde reductase could still bind to a blue dextran-Sepharose 4B column suggesting that the modified arginine did not bind NADPH. Sepharose 75-84 aldo-keto reductase family 1 member B Sus scrofa 21-39 312969-4 1979 Crossed antigen-antibody electrophoresis using agarose in both steps, immunoelectrophoresis, and agarose electrophoresis followed by immunofixation all revealed a slow-moving alpha1-antitrypsin, cathodal to the Pi Z region. Sepharose 97-104 serpin family A member 1 Homo sapiens 175-193 36151-1 1979 Aldose reductase (alditol:NADP+ 1-oxidoreductase, EC 1.1.1.21) has been purified 1500-fold from porcine brain in a four-step procedure employing Blue-Sepharose 6B affinity chromatography. Sepharose 150-159 aldo-keto reductase family 1 member B Homo sapiens 0-16 34612-4 1979 At neutral pH and low ionic strength, lactoperoxidase is adsorbed on insolubilized diiodotyrosine (diiodotyrosine-agarose). Sepharose 114-121 lactoperoxidase Homo sapiens 38-53 34612-7 1979 Lactoperoxidase is also adsorbed on insolubilized thiols (thiol-agarose). Sepharose 64-71 lactoperoxidase Homo sapiens 0-15 429278-3 1979 It was co-polymerized with rabbit actin and purified by affinity chromatography on DNase I-Sepharose. Sepharose 91-100 deoxyribonuclease-1 Oryctolagus cuniculus 83-90 35293-1 1979 The IgG fraction of antiserum directed against gamma-glutamyl transpeptidase (gammaGTP, EC 2.3.2.2) isolated from human kidney brush border membranes after limited proteolysis, was covalently bound to cyanogen bromide-activated Sepharose. Sepharose 228-237 inactive glutathione hydrolase 2 Homo sapiens 47-76 435577-1 1979 Several human granulocyte proteinases sensitive to the thermo- and acid-resistant proteinase inhibitor from rabbit serum (TASPI) were revealed, using TASPI-Sepharose 4B. Sepharose 156-165 endogenous retrovirus group K member 25 Homo sapiens 26-36 438156-1 1979 A binding protein with apparent specificity for beta-glucuronidase has been partially purified from a Triton X-100 extract of rat liver microsomes by affinity chromatography on glucuronidase-Sepharose 2B. Sepharose 191-200 glucuronidase, beta Rattus norvegicus 48-66 86384-6 1979 Agarose electrophoresis of the TNF from mouse and human serum indicated the principle components were alpha 1-alpha 2 globulins. Sepharose 0-7 tumor necrosis factor Mus musculus 31-34 219006-3 1979 The hEGF/hUG in homogenates of human salivary glands and a wide variety of other endocrine and nonendocrine tissues was extracted by Amberlite CG-50 cation exchange chromatography and immune affinity chromatography using the immunoglobulin fraction of rabbit anti-hEGF serum covalently bound to agarose. Sepharose 295-302 epidermal growth factor Homo sapiens 4-8 224861-17 1979 By chromatography on a Sepharose-calmodulin affinity column evidence was obtained for the formation of a Ca2+-dependent complex between calmodulin and myosin light-chain kinase. Sepharose 23-32 calmodulin Oryctolagus cuniculus 33-43 224861-17 1979 By chromatography on a Sepharose-calmodulin affinity column evidence was obtained for the formation of a Ca2+-dependent complex between calmodulin and myosin light-chain kinase. Sepharose 23-32 calmodulin Oryctolagus cuniculus 136-146 224861-17 1979 By chromatography on a Sepharose-calmodulin affinity column evidence was obtained for the formation of a Ca2+-dependent complex between calmodulin and myosin light-chain kinase. Sepharose 23-32 myosin light chain kinase, smooth muscle Oryctolagus cuniculus 151-176 88048-5 1979 Hybridization of gastrin cDNA or synthetic dodecanucleotide to hog antral RNA separated by gel electrophoresis on agarose gels in the presence of methylmercuric hydroxide indicates that the mRNA coding for gastrin is about 620 nucleotides long. Sepharose 114-121 gastrin Homo sapiens 17-24 221912-5 1979 The extract was applied to an immune affinity chromatography column consisting of affinity-purified (1-24)ACTH antiserum covalently bound to agarose. Sepharose 141-148 proopiomelanocortin Homo sapiens 106-110 217872-2 1979 The enzyme succinic semialdehyde dehydrogenase from pig brain has been 2000-fold purified by a combination of DEAE-cellulose, hydroxyapatite, and AMP-Sepharose chromatography. Sepharose 150-159 aldehyde dehydrogenase 5 family member A1 Sus scrofa 11-46 372243-3 1979 Platelet and plasma fibronectin were bound and eluted from gelatin-sepharose under similar conditions. Sepharose 67-76 fibronectin 1 Homo sapiens 20-31 109083-2 1979 The high specificity of interaction between the thiamin-binding protein and the riboflavin-binding protein of the egg white, with a protein/protein molar ratio of 1.0, led to the development of an alternative procedure that used the riboflavin-binding protein immobilized on CNBr-activated Sepharose as the affinity matrix. Sepharose 290-299 riboflavin binding protein Gallus gallus 233-259 107261-3 1979 Both the directly suppressive activity of the GAT-TsF and the ability of GAT-TsF to induce new suppressor T cells (Ts2) in vitro were adsorbed to and fully recoverable from the guinea pig anti-CGAT-Sepharose immunoadsorbent, while the TsF passed through the control NGPS-Sepharose without appreciable binding. Sepharose 198-207 glycine-N-acyltransferase Mus musculus 73-76 107261-3 1979 Both the directly suppressive activity of the GAT-TsF and the ability of GAT-TsF to induce new suppressor T cells (Ts2) in vitro were adsorbed to and fully recoverable from the guinea pig anti-CGAT-Sepharose immunoadsorbent, while the TsF passed through the control NGPS-Sepharose without appreciable binding. Sepharose 271-280 glycine-N-acyltransferase Mus musculus 73-76 383819-3 1979 Specificity of the antisera for A1AT was established by subsequent solid phase immunoadsorption against normal human serum bound to AH-Sepharose 4B. Sepharose 135-144 serpin family A member 1 Homo sapiens 32-36 286301-2 1979 Chicken cellular fibronectin isolated from cultured embryonic fibroblasts was permitted to bind to gelatin coupled to agarose beads and was then digested extensively with chymotrypsin. Sepharose 118-125 fibronectin 1 Gallus gallus 17-28 286301-3 1979 A prominent 40,000-dalton fragment of fibronectin consisting of a single polypeptide chain was detected by sodium dodecyl sulfate/polyacrylamide gel electrophoresis of material remaining bound to the gelatin-agarose. Sepharose 208-215 fibronectin 1 Gallus gallus 38-49 83915-1 1979 The majority of melanoma tumor antigen activity present in melanoma extracts derived from fresh tumor tissue binds to a Sepharose-anti-beta2-microglobulin adsorbent. Sepharose 120-129 beta-2-microglobulin Homo sapiens 135-154 218985-1 1979 A RIA has been developed for somatomedin A (SM-A) utilizing Sepharose-bound antibodies. Sepharose 60-69 survival of motor neuron 1, telomeric Homo sapiens 29-42 218985-1 1979 A RIA has been developed for somatomedin A (SM-A) utilizing Sepharose-bound antibodies. Sepharose 60-69 survival of motor neuron 1, telomeric Homo sapiens 44-48 437700-2 1979 Using the technique of affinity chromatography on a myo-inositol-substituted Sepharose, the myo-inositol oxygenase from rat kidneys was purified to homogeneity. Sepharose 77-86 myo-inositol oxygenase Rattus norvegicus 96-114 532487-1 1979 The isoenzyme pattern of hexokinase in rabbit red cells (erythrocytes, fetal erythrocytes and reticulocytes) were determined by means of agarose gel and disc electrophoresis. Sepharose 137-144 hexokinase-2 Oryctolagus cuniculus 25-35 373497-0 1979 Large-scale preparation of phosphoglycerate kinase from Saccharomyces cerevisiae using Cibacron Blue-Sepharose 4B pseudoaffinity chromatography. Sepharose 101-110 phosphoglycerate kinase Saccharomyces cerevisiae S288C 27-50 438695-0 1979 Extraction of small amounts of oxytocin from biological fluids by means of agarose-bound neurophysin. Sepharose 75-82 oxytocin/neurophysin I prepropeptide Homo sapiens 31-39 438695-1 1979 The use of agarose-bound neurophysin for the extraction of oxytocin from biological fluids is described. Sepharose 11-18 oxytocin/neurophysin I prepropeptide Homo sapiens 59-67 117659-1 1979 Cytochrome P-450 and NADPH-cytochrome P-450 reductase were covalently attached to Sepharose 4B in different ways in order to find out factors which are important for the organization of the individual components to a catalytically active system. Sepharose 82-94 cytochrome P450 family 4 subfamily F member 3 Homo sapiens 0-16 117659-1 1979 Cytochrome P-450 and NADPH-cytochrome P-450 reductase were covalently attached to Sepharose 4B in different ways in order to find out factors which are important for the organization of the individual components to a catalytically active system. Sepharose 82-94 cytochrome p450 oxidoreductase Homo sapiens 21-53 162260-3 1979 ATPase A and ATPase B appear to be distinct enzymes as judged from their elution profiles obtained after DEAE-cellulose and ATP-Sepharose column chromatography, from their behavior towards actinomycin D, a DNA intercalating agent, and from their sensitivity to monovalent salt concentration. Sepharose 128-137 ATPase phospholipid transporting 8A2 Homo sapiens 13-21 162260-3 1979 ATPase A and ATPase B appear to be distinct enzymes as judged from their elution profiles obtained after DEAE-cellulose and ATP-Sepharose column chromatography, from their behavior towards actinomycin D, a DNA intercalating agent, and from their sensitivity to monovalent salt concentration. Sepharose 128-137 ATPase phospholipid transporting 8A2 Homo sapiens 0-3 231981-4 1979 The CRP prepared according to this method is able to form precipitating complexes in agarose with rabbit lipoproteins. Sepharose 85-92 C-reactive protein Oryctolagus cuniculus 4-7 231981-6 1979 It is demonstrated that CRP-High Density Lipoproteins (HLD) association produces a second precipitation arc when the pure CRP is revealed by a specific antiserum in agarose. Sepharose 165-172 C-reactive protein Oryctolagus cuniculus 24-27 231981-6 1979 It is demonstrated that CRP-High Density Lipoproteins (HLD) association produces a second precipitation arc when the pure CRP is revealed by a specific antiserum in agarose. Sepharose 165-172 C-reactive protein Oryctolagus cuniculus 122-125 520650-1 1979 Cultured mouse peritoneal macrophages were shown to secrete the lysosomal enzyme N-acetyl-glucosaminidase (N-ac-Glu) in response to IgG-Sepharose and some other non-endocytosable particles without substantial release of the cytoplasmic enzyme, lactate dehydrogenase. Sepharose 136-145 alpha-N-acetylglucosaminidase (Sanfilippo disease IIIB) Mus musculus 81-105 520650-1 1979 Cultured mouse peritoneal macrophages were shown to secrete the lysosomal enzyme N-acetyl-glucosaminidase (N-ac-Glu) in response to IgG-Sepharose and some other non-endocytosable particles without substantial release of the cytoplasmic enzyme, lactate dehydrogenase. Sepharose 136-145 alpha-N-acetylglucosaminidase (Sanfilippo disease IIIB) Mus musculus 107-115 114594-2 1979 A simple electrophoretic technique employing commercially available agarose films is described for the routine estimation of plasma complement C3 conversion. Sepharose 68-75 complement C3 Homo sapiens 132-145 422651-5 1979 (c) Core glycosylation of in vitro-synthesized PE2 and E1 forms, as indicated by binding to concanavalin A Sepharose and subsequent elution by alpha-methylmannoside. Sepharose 107-116 ETS2 repressor factor Homo sapiens 47-50 89171-0 1979 Agarose isoelectric focusing of native human immunoglobulin M and alpha 2-macroglobulin. Sepharose 0-7 alpha-2-macroglobulin Homo sapiens 66-87 89171-1 1979 The resolution of native 19S IgM and alpha 2-macroglobulin by agarose isoelectric focusing is described. Sepharose 62-69 alpha-2-macroglobulin Homo sapiens 37-58 438530-2 1979 Donor strain TMP10 contained numerous species of extrachromosomal DNA ranging from approximately 2 x 10(6) to 20 x 10(6) daltons in molecular weight, as revealed by agarose gel electrophoresis. Sepharose 165-172 oligodendrocytic myelin paranodal and inner loop protein Homo sapiens 13-18 512360-4 1979 The haptoglobin-hemoglobin complexes are then specifically eluted by buffers containing dithiothreitol or other thiols and are further purified by chromatography on concanavalin A-agarose and Sephacryl S-200 columns. Sepharose 180-187 haptoglobin Homo sapiens 4-15 222997-0 1979 Separation of right-side-out and inside-out submitochondrial particles by affinity chromatography on Sepharose-cytochrome c. Sepharose 101-110 cytochrome c, somatic Homo sapiens 111-123 493880-1 1979 Neuraminidase-treated lymphocytes from the peripheral blood of normal human donors were fractionated on columns charged with Helix pomatia haemagglutinin (HP) coupled to Sepharose 4B. Sepharose 170-179 neuraminidase 1 Homo sapiens 0-13 471122-4 1979 The most heterogeneous CEA preparation was further purified by the affinity chromatography on Concanavalin A-Sepharose 4B and by DEAE-Sephadex A-50 chromatography and the usefulness of these procedures for purification of CEA is compared. Sepharose 109-121 CEA cell adhesion molecule 3 Homo sapiens 23-26 93753-0 1979 Measurement of androgen receptor in cytosol of human prostatic tissues with a sepharose-linked antibody system. Sepharose 78-87 androgen receptor Homo sapiens 15-32 504286-0 1979 Affinity chromatography with agarose-lectin derivatives: separation of human glycoproteins and application to erythropoietin purification. Sepharose 29-36 erythropoietin Homo sapiens 110-124 233696-6 1978 None of these IR-CT materials was extracted from DMS-79 medium by affinity chromatography using an ACTH antibody covalently bound to agarose. Sepharose 133-140 proopiomelanocortin Homo sapiens 99-103 213426-4 1978 Using system involving Sepharose-bound HDL, it could be shown that not only free apoprotein molecules but subunits consisting of lipid-apoprotein combinations were exchanged between HDL2 and HDL3. Sepharose 23-32 junctophilin 3 Homo sapiens 182-186 281544-4 1978 The cRABP of human origin (normal uterus and neoplastic mammary tissue) differed in its mobility in agarose electrophoresis from that of rat testis cRABP. Sepharose 100-107 cellular retinoic acid binding protein 1 Homo sapiens 4-9 213426-4 1978 Using system involving Sepharose-bound HDL, it could be shown that not only free apoprotein molecules but subunits consisting of lipid-apoprotein combinations were exchanged between HDL2 and HDL3. Sepharose 23-32 HDL3 Homo sapiens 191-195 363168-6 1978 Unlike renal renin, renin in human amniotic fluid was not a glycoprotein and behaved similarly on concanavalin A-Sepharose before and after activation by pepsin. Sepharose 113-122 renin Homo sapiens 20-25 718970-2 1978 Malonyl-CoA decarboxylase (EC 4.1.1.9) was purified 500--600-fold from the mammary gland extracts by (NH4)2SO4 precipitation, gel filtration with Sepharose 4B, anion-exchange chromatography with QAE-Sephadex, and chromatography with NADP-Agarose. Sepharose 146-158 malonyl-CoA decarboxylase Rattus norvegicus 0-25 109102-3 1978 5"-Methylthioadenosine phosphorylase has been purified approximately 340-fold in 20% yield from human prostate: the use of affinity chromatography by Sepharose-Hg has been found particularly advantageous. Sepharose 150-159 methylthioadenosine phosphorylase Homo sapiens 0-36 215508-3 1978 The lysates of the hybrid cells were treated with Sepharose-coupled antihuman alpha-galactosidase B and the activity of the adsorbed enzyme was measured on the Sepharose beads as N-acetyl-alpha-galactosaminidase. Sepharose 50-59 alpha-N-acetylgalactosaminidase Homo sapiens 78-99 215032-1 1978 A "sinking" pre-beta lipoprotein was sought in a probability sample of 1854, 50--72-year-old men of Japanese ancestry in Honolulu by ultracentrifugation of plasma and electrophoresis of the bottom fraction (density greater than 1.006) in agarose. Sepharose 238-245 prolactin regulatory element binding Homo sapiens 12-20 569762-2 1978 One of the Sepharose 4B peaks was shown to contain RNA with a molecular weight reported for vitellogenin mRNA (approximately 34S). Sepharose 11-20 a1-a Xenopus laevis 92-104 154322-1 1978 The primary inhibitor of plasmin in human plasma was purified by a four-step procedure involving fractional (NH(4))(2)SO(4) precipitation, ion-exchange chromatography on a column of DEAE-Sepharose CL-6B and affinity chromatography on both a plasminogen-CH-Sepharose 4B column and a column of 6-aminohexanoic acid covalently coupled through the carboxylate function to AH-Sepharose 4B. Sepharose 187-196 plasminogen Homo sapiens 25-32 9762089-4 1978 A two-step separation and purification of elastin from the collagen-free extract is based on absorption of the acidic proteins on DEAE-cellulose and gel filtration through agarose. Sepharose 172-179 LOC100620140 Sus scrofa 42-49 154322-1 1978 The primary inhibitor of plasmin in human plasma was purified by a four-step procedure involving fractional (NH(4))(2)SO(4) precipitation, ion-exchange chromatography on a column of DEAE-Sepharose CL-6B and affinity chromatography on both a plasminogen-CH-Sepharose 4B column and a column of 6-aminohexanoic acid covalently coupled through the carboxylate function to AH-Sepharose 4B. Sepharose 256-265 plasminogen Homo sapiens 25-32 213215-1 1978 An affinity chromatographic method using concanavalin A-Sepharose is described for the determination of N-acetyl-beta-D-glucosaminidase, arylsulfatase. Sepharose 56-65 O-GlcNAcase Homo sapiens 104-135 730749-1 1978 The interaction of beta-trypsin [EC 3.4.21.4] immobilized on Sepharose with competitive inhibitors was quantitatively studied by frontal affinity chromatography. Sepharose 61-70 serine protease 1 Homo sapiens 19-31 81850-3 1978 The remaining antibodies from this serum and gamma-globulin from a sheep antiserum against rabbit AFP, without prior absorption, were coupled to Sepharose. Sepharose 145-154 alpha-fetoprotein Oryctolagus cuniculus 98-101 153346-0 1978 Purification of modulator-deficient myosin light-chain kinase by modulator protein-Sepharose affinity chromatography. Sepharose 83-92 myosin light chain kinase, smooth muscle Oryctolagus cuniculus 36-61 153346-1 1978 Modulator-deficient myosin light-chain kinase from rabbit skeletal muscle was purified by modulator protein-Sepharose 4B affinity chromatography. Sepharose 108-120 myosin light chain kinase, smooth muscle Oryctolagus cuniculus 20-45 739266-2 1978 In the present study this is shown also by incubating neurons with Sepharose 4B or methylacrylate spherules to which were coupled anti-S-100 antibodies. Sepharose 67-79 S100 calcium binding protein B Homo sapiens 135-140 736895-3 1978 A previously described, freeze-dried, partially purified ram acrosin preparation was fractionated on a column of Sepharose linked to the acrosin inhibitor p-(p"-aminophenoxypropoxy)benzamidine. Sepharose 113-122 acrosin Homo sapiens 137-144 19605248-1 1978 Cathepsin D (EC 3.4.3.23) purified from human putuitary by affinity chromatography on pepstatin-Sepharose cleaved human beta-endorphin (LPH 61-91) at the Leu(77)-Phe(78) bond after incubation at pH 3.2 for 1-3. Sepharose 96-105 cathepsin D Homo sapiens 0-11 19605248-1 1978 Cathepsin D (EC 3.4.3.23) purified from human putuitary by affinity chromatography on pepstatin-Sepharose cleaved human beta-endorphin (LPH 61-91) at the Leu(77)-Phe(78) bond after incubation at pH 3.2 for 1-3. Sepharose 96-105 proopiomelanocortin Homo sapiens 120-134 736895-14 1978 When beta-acrosin was passed through a column of Sepharose linked to the non-inhibitory deamidinated analogue of the inhibitor it behaved as a hydrophobic protein. Sepharose 49-58 acrosin Homo sapiens 10-17 736901-1 1978 Soluble fibronectin of human plasma was specifically dissociated at neutral pH from gelatin-agarose by several cationic amino compounds, notably the polyamines spermine, spermidine and putrescine, the basic amino acid arginine, and amino sugars. Sepharose 92-99 fibronectin 1 Homo sapiens 8-19 365308-3 1978 Determination and identification of beta-lactamase activity were achieved by combining several up-to-date methods including (i) neutralization by anti-beta-lactamase sera, (ii) purification by affinity chromatography on cephalosporin C linked Sepharose 4B, (iii) determination of substrate specificity and kinetic values (Km and Vmax) by a computerized microacidimetric method, and (iv) isoelectric focusing. Sepharose 243-252 beta-lactamase Escherichia coli 36-50 711708-1 1978 For the isolation of human lysozyme from the urine of leukemia patients, a simple method has been established which involves precipitation of urinary proteins by 60% saturation with ammonium sulfate, fractionation of crude lysozyme on Sephadex G-50 and purification by CM-Sepharose chromatography. Sepharose 272-281 lysozyme Homo sapiens 27-35 101631-4 1978 Fibronectin was isolated from human CSF by affinity chromatography on Sepharose-coupled gelatin and was further analyzed by SDS-polyacrylamide gel electrophoresis. Sepharose 70-79 fibronectin 1 Homo sapiens 0-11 359704-1 1978 The responsiveness to macrophage migration inhibitory factor (MIF) of peritoneal exudate cells (PEC) from the LPS unresponsive C3H/HeJ and C57BL/10ScCR mice was assessed by the indirect agarose microdroplet macrophage migration inhibition assay. Sepharose 186-193 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 22-60 283388-2 1978 alpha-LA mRNA was homogeneous as judged by electrophoresis in urea/agarose gel and in 1.5% agarose gel under glyoxal-denaturation conditions, which gave a molecular weight of 210,000. Sepharose 67-74 lactalbumin, alpha Rattus norvegicus 0-8 283388-2 1978 alpha-LA mRNA was homogeneous as judged by electrophoresis in urea/agarose gel and in 1.5% agarose gel under glyoxal-denaturation conditions, which gave a molecular weight of 210,000. Sepharose 91-98 lactalbumin, alpha Rattus norvegicus 0-8 367363-6 1978 The key to each purification was the use of a Sepharose-N-aminoacylglucosamine affinity matrix in which a high degree of specificity for a particular hexokinase isoenzyme could be introduced by either varying the length of the aminoacyl spacer and/or varying the ligand concentration coupled to the gel. Sepharose 46-55 hexokinase Saccharomyces cerevisiae S288C 150-160 81690-5 1978 We have been able to correlate the pattern of alpha-fetoprotein heterogeneity seen following extended agarose gel electrophoresis with that obtained during isoelectric focusing in the presence of urea. Sepharose 102-109 alpha fetoprotein Homo sapiens 46-63 367363-10 1978 All derivatives were competitive inhibitors, with respect to glucose, of the hexokinase reaction, and there was a direct correlation between the Ki for a particular derivative and its ability to act as an affinity matrix when immobilized to CNBr-activated Sepharose 4B. Sepharose 256-265 hexokinase Saccharomyces cerevisiae S288C 77-87 367363-11 1978 Muscle hexokinase type II could be chromatographed on the Sepharose conjugates of all four N-aminoacylglucosamine derivatives, although the N-aminohexanoylglucosamine derivative proved best. Sepharose 58-67 hexokinase 2 Rattus norvegicus 7-25 708776-2 1978 Squid rhodopsin was extracted with solutions of fatty acid esters of sucrose (monolaurate and monostearate) and purified by DEAE-cellulose and concanavalin A-Sepharose affinity chromatography. Sepharose 158-167 rhodopsin Homo sapiens 6-15 213050-1 1978 Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. Sepharose 158-167 lactate dehydrogenase C Homo sapiens 16-21 708755-4 1978 The TPO protein, isolated by gel filtration in 2% SDS on an agarose column, like the PO protein, is highly insoluble in aqueous solvents. Sepharose 60-67 LOW QUALITY PROTEIN: thyroid peroxidase Oryctolagus cuniculus 4-7 701439-4 1978 The most efficient purification was obtained when the enzyme was eluted from a column containing the acetylcholinesterase inhibitor [1-methyl-9-(Nbeta-epsilon-amino-caproyl)-beta-aminopropylamino] acridinium bromide hydrobromide covalently linked to Sepharose 4B. Sepharose 250-259 acetylcholinesterase (Yt blood group) Sus scrofa 101-121 213050-1 1978 Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. Sepharose 158-167 lactate dehydrogenase C Homo sapiens 23-56 213050-1 1978 Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. Sepharose 176-185 lactate dehydrogenase C Homo sapiens 16-21 213050-1 1978 Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. Sepharose 176-185 lactate dehydrogenase C Homo sapiens 23-56 31971-1 1978 The interactions of H1 (H1A, H1B), H2A, H2B, H3, H4, and H5 with phenyl cross-linked agarose were studied. Sepharose 85-92 H1.1 linker histone, cluster member Homo sapiens 24-27 31971-1 1978 The interactions of H1 (H1A, H1B), H2A, H2B, H3, H4, and H5 with phenyl cross-linked agarose were studied. Sepharose 85-92 H1.5 linker histone, cluster member Homo sapiens 29-32 31971-1 1978 The interactions of H1 (H1A, H1B), H2A, H2B, H3, H4, and H5 with phenyl cross-linked agarose were studied. Sepharose 85-92 H2A clustered histone 18 Homo sapiens 35-38 31971-1 1978 The interactions of H1 (H1A, H1B), H2A, H2B, H3, H4, and H5 with phenyl cross-linked agarose were studied. Sepharose 85-92 H2B clustered histone 21 Homo sapiens 40-43 308929-7 1978 Mitogenic activity of SPA coupled to Sepharose beads was different from that of soluble SPA and paralleled that of StaCw. Sepharose 37-46 surfactant protein A1 Homo sapiens 22-25 364884-1 1978 Protein T was isolated from type 12 Streptococcus pyogenes cell wall by digestion with CNBr-sepharose linked trypsin and purified by ion exchange chromatography on QAE Sephadex A-50. Sepharose 92-101 brachyury, T-box transcription factor T Mus musculus 0-9 31050-2 1978 In selected cases with unsure CSF oligoclonal bands on agarose gel electrophoresis, isoelectric focusing displayed definite oligoclonal bands. Sepharose 55-62 colony stimulating factor 2 Homo sapiens 30-33 706495-3 1978 Finding concerning the interaction of the platelets with fibrinogen connected to sepharose plead for the fact that a change of conformation in the molecule of fibrinogen precedes the specific platelet reaction. Sepharose 81-90 fibrinogen beta chain Homo sapiens 57-67 706495-3 1978 Finding concerning the interaction of the platelets with fibrinogen connected to sepharose plead for the fact that a change of conformation in the molecule of fibrinogen precedes the specific platelet reaction. Sepharose 81-90 fibrinogen beta chain Homo sapiens 159-169 99166-3 1978 Unique to this purification scheme is the batch use of insolubilized hemoglobin--Sepharose beads to remove the ubiquitous contaminant haptoglobin. Sepharose 81-90 haptoglobin Canis lupus familiaris 134-145 150291-2 1978 Myosin from gizzards of 15-day-old chicken embryos was highly purified by ammonium sulfate fractionation in the presence of ATP and MgCl2, ultra-centrifugation and Sepharose 4B chromatography. Sepharose 164-173 myosin, heavy chain 15 Gallus gallus 0-6 358857-0 1978 Purification of orotate phosphoribosyltransferase and orotidylate decarboxylase by affinity chromatography on Sepharose dye derivatives. Sepharose 110-119 uridine monophosphate synthetase Homo sapiens 16-49 689031-7 1978 NAD+ immobilized on agarose through the C-8 of the adenine ring is a superior substrate compared with NAD+ linked to agarose via its periodate-oxidized ribose moieties. Sepharose 20-27 homeobox C8 Homo sapiens 40-43 681752-3 1978 They also differ in their sensitivity to phospholipase C. The detergent solubilized monomer IgG2a FcR can be removed from solution with a Sepharose IgG2a column, has an S value of 4 to 5 and is resistant to phospholipase C. The solubilized aggregated IgG FcR does not bind to insolubilized IgG2a, has an S value of greater than 19, and is sensitive to phospholipase C treatment. Sepharose 138-147 immunoglobulin heavy variable V1-9 Mus musculus 92-97 681752-3 1978 They also differ in their sensitivity to phospholipase C. The detergent solubilized monomer IgG2a FcR can be removed from solution with a Sepharose IgG2a column, has an S value of 4 to 5 and is resistant to phospholipase C. The solubilized aggregated IgG FcR does not bind to insolubilized IgG2a, has an S value of greater than 19, and is sensitive to phospholipase C treatment. Sepharose 138-147 Fc receptor Mus musculus 98-101 681752-3 1978 They also differ in their sensitivity to phospholipase C. The detergent solubilized monomer IgG2a FcR can be removed from solution with a Sepharose IgG2a column, has an S value of 4 to 5 and is resistant to phospholipase C. The solubilized aggregated IgG FcR does not bind to insolubilized IgG2a, has an S value of greater than 19, and is sensitive to phospholipase C treatment. Sepharose 138-147 immunoglobulin heavy variable V1-9 Mus musculus 148-153 681752-3 1978 They also differ in their sensitivity to phospholipase C. The detergent solubilized monomer IgG2a FcR can be removed from solution with a Sepharose IgG2a column, has an S value of 4 to 5 and is resistant to phospholipase C. The solubilized aggregated IgG FcR does not bind to insolubilized IgG2a, has an S value of greater than 19, and is sensitive to phospholipase C treatment. Sepharose 138-147 immunoglobulin heavy variable V1-9 Mus musculus 148-153 318209-1 1978 Optimal conditions have been determined for the coupling of rat liver phenylalanine hydroxylase (Phe H) to cyanogen bromide-Sepharose 4B. Sepharose 124-133 phenylalanine hydroxylase Rattus norvegicus 70-95 318209-1 1978 Optimal conditions have been determined for the coupling of rat liver phenylalanine hydroxylase (Phe H) to cyanogen bromide-Sepharose 4B. Sepharose 124-133 phenylalanine hydroxylase Rattus norvegicus 97-102 356051-3 1978 The protein in the vesicles was antigenically active, as demonstrated by specific binding to anti-beta2-microglobulin IgG-Sepharose beads and by specific inhibition of alloantibody and complement-mediated cytotoxicity. Sepharose 122-131 beta-2-microglobulin Homo sapiens 98-117 659428-2 1978 S-adenosyl-methionine:mRNA (guanine-7)-methyltransferase copurified with guanylyltransferase activity through chromatography on DNA agarose, phosphocellulose, and centrifugation in glycerol gradients, suggesting that the two activities are closely associated. Sepharose 132-139 RNA (guanine-7-) methyltransferase Mus musculus 22-56 353285-12 1978 Three hours after the injection, renal citrate synthase was isolated by ATP-sepharose column chromatography and immuno-precipitation with the specific antiserum. Sepharose 76-85 citrate synthase Rattus norvegicus 39-55 353285-15 1978 Mitochondrial citrate synthase was isolated either by ATP-sepharose column chromatography and immuno-precipitation, or by polyacrylamide gel electrophoresis. Sepharose 58-67 citrate synthase Rattus norvegicus 14-30 351754-0 1978 Differences in the isoelectric focussing pattern of antibodies to human serum albumin eluted from an immunoadsorbent (HSA-sepharose) with thiocyanate ions. Sepharose 122-131 albumin Homo sapiens 78-85 655428-0 1978 Purification of thiosulfate sulfurtransferase by selective immobilization on blue agarose. Sepharose 82-89 thiosulfate sulfurtransferase Homo sapiens 16-45 26697-12 1978 Concanavalin A and ricin Sepharose affinity chromatography showed that TeBG is bound to the columns nearly quantitatively, whereas hABP is bound to the columns only partially. Sepharose 25-34 sex hormone binding globulin Homo sapiens 71-75 231981-2 1979 This preparation takes advantage of the calcium-dependent affinity of CRP for an agarose gel bearing 2-aminoethanol dihydrogen-phosphate as a ligand. Sepharose 81-88 C-reactive protein Oryctolagus cuniculus 70-73 209913-1 1978 By visual inspection of agarose gel electrophoretograms of lipoproteins, a beta lipoprotein with increased mobility, here named "rapid beta" lipoprotein, has been observed. Sepharose 24-31 amyloid beta precursor protein Homo sapiens 73-79 686071-1 1978 Ovarian tumor-associated antigen isolated from human tumor tissue was shown to have a different mobility from that of carcinoembryonic antigen (CEA) in both acrylamide gel electrophoresis and immunoelectrophoresis in agarose. Sepharose 217-224 CEA cell adhesion molecule 3 Homo sapiens 118-142 686071-1 1978 Ovarian tumor-associated antigen isolated from human tumor tissue was shown to have a different mobility from that of carcinoembryonic antigen (CEA) in both acrylamide gel electrophoresis and immunoelectrophoresis in agarose. Sepharose 217-224 CEA cell adhesion molecule 3 Homo sapiens 144-147 211029-2 1978 Poly(ADP-ribose) polymerase from Ehrlich ascites tumor cells, partially purified by chromatography on DNA-agarose, was obtained as a more than 80% homogeneous preparation by isoelectric focusing in a sucrose gradient. Sepharose 106-113 poly (ADP-ribose) polymerase family, member 1 Mus musculus 0-27 98583-3 1978 The culture supernatants were tested for the presence of LIF by the agarose migration method. Sepharose 68-75 LIF interleukin 6 family cytokine Homo sapiens 57-60 694235-1 1978 Human platelet plasma membrane glycoprotein I with an apparent molecular weight of approximately 150,000 has been shown to be one of the proteins retained by thrombin immobilized on Sepharose 4B. Sepharose 182-191 coagulation factor II, thrombin Homo sapiens 158-166 78727-2 1978 Bovine alpha1-fetoprotein was isolated from fetal calf serum by successive procedures of concanavalin A-Sepharose chromatography, DEAE-Sephadex chromatography, SP-Sephadex chromatography and preparative disc polyacrylamide gel electrophoresis. Sepharose 104-113 alpha fetoprotein Bos taurus 7-25 710642-4 1978 When LATS positive serum was fractionated by affinity chromatography on a Sepharose-bound antibody against human IgG, Fab of IgG and Fc of IgG, LATS activity was always retained in IgG fraction. Sepharose 74-83 FA complementation group B Homo sapiens 118-121 710642-10 1978 When the Fab fragment of IgG (1) was separated from papain hydrolysed IgG (1), using a Protein A-bound Sepharose column, a short-acting type of thyroid stimulating activity was found in only this fraction. Sepharose 103-112 FA complementation group B Homo sapiens 9-12 95911-1 1978 An attempt was made to develop a method to isolate directly the fibrinogenfibrin (FDP and/or fdp) degradation products from plasma by means of small chromatographic columns of activated Sepharose 4-B coupled with antifibrinogen serum. Sepharose 186-199 otoraplin Homo sapiens 82-85 657550-1 1978 The affinity adsorbent Cibacron Blue F3GA-Sepharose 4-B has been used to develop a binding assay for human serum albumin. Sepharose 42-55 albumin Homo sapiens 107-120 28768-1 1978 Yeast glyceraldehyde-3-phosphate dehydrogenase (glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12) immobilized on CNBr-activated Sepharose 4-B has been subjected to dissociation to obtain matrix-bound dimeric species of the enzyme. Sepharose 157-166 glyceraldehyde-3-phosphate dehydrogenase Rattus norvegicus 6-46 29675-0 1978 [Immobilization of glyceraldehyde-3-phosphate dehydrogenase by non-covalent binding to specific antibodies and Fab-fragments coupled to Sepharose]. Sepharose 136-145 glyceraldehyde-3-phosphate dehydrogenase Rattus norvegicus 19-59 29675-1 1978 Rabbit antibodies to rat skeletal muscle glyceraldehyde-3-phosphate dehydrogenase, as well as monovalent Fab fragments of these antibodies were coupled to CNBr-activated Sepharose 4B. Sepharose 170-179 glyceraldehyde-3-phosphate dehydrogenase Rattus norvegicus 41-81 209919-1 1978 The interaction of isolated Lp(a) lipoprotein or other lipoprotein classes with different glycosaminoglycans (GAG) bound to activated Sepharose was studied. Sepharose 134-143 lipoprotein(a) Homo sapiens 28-33 705688-3 1978 Purified factor VIII and different amounts of thrombin complexed to Sepharose 4 B were mixed and incubated for various periods of time. Sepharose 68-77 coagulation factor II, thrombin Homo sapiens 46-54 705688-6 1978 Evidences for a thrombin-induced potentiation of the factor VIII activity, seen only in the thrombin-sepharose containing test samples analyzed by the one-stage method, are here interpreted as thrombin-effects peculiar to this factor VIII test system and not as potentiation by thrombin of the factor itself. Sepharose 101-110 coagulation factor II, thrombin Homo sapiens 16-24 705688-6 1978 Evidences for a thrombin-induced potentiation of the factor VIII activity, seen only in the thrombin-sepharose containing test samples analyzed by the one-stage method, are here interpreted as thrombin-effects peculiar to this factor VIII test system and not as potentiation by thrombin of the factor itself. Sepharose 101-110 coagulation factor II, thrombin Homo sapiens 92-100 705688-6 1978 Evidences for a thrombin-induced potentiation of the factor VIII activity, seen only in the thrombin-sepharose containing test samples analyzed by the one-stage method, are here interpreted as thrombin-effects peculiar to this factor VIII test system and not as potentiation by thrombin of the factor itself. Sepharose 101-110 coagulation factor II, thrombin Homo sapiens 92-100 705688-6 1978 Evidences for a thrombin-induced potentiation of the factor VIII activity, seen only in the thrombin-sepharose containing test samples analyzed by the one-stage method, are here interpreted as thrombin-effects peculiar to this factor VIII test system and not as potentiation by thrombin of the factor itself. Sepharose 101-110 coagulation factor II, thrombin Homo sapiens 92-100 705690-1 1978 Human antithrombin III was purified from fresh human plasma by affinity chromatography on heparin-Sepharose, affinity chromatography on concanavalin A Sepharose, gel filtration on Ultrogel AcA 34, ion exchange chromatography on DEAE A-50 Sephadex and preparative agarose gel electrophoresis. Sepharose 98-107 serpin family C member 1 Homo sapiens 6-22 705690-1 1978 Human antithrombin III was purified from fresh human plasma by affinity chromatography on heparin-Sepharose, affinity chromatography on concanavalin A Sepharose, gel filtration on Ultrogel AcA 34, ion exchange chromatography on DEAE A-50 Sephadex and preparative agarose gel electrophoresis. Sepharose 151-160 serpin family C member 1 Homo sapiens 6-22 705690-1 1978 Human antithrombin III was purified from fresh human plasma by affinity chromatography on heparin-Sepharose, affinity chromatography on concanavalin A Sepharose, gel filtration on Ultrogel AcA 34, ion exchange chromatography on DEAE A-50 Sephadex and preparative agarose gel electrophoresis. Sepharose 263-270 serpin family C member 1 Homo sapiens 6-22 355597-6 1978 The radioactive fibronectin present in the endothelial postculture medium and in urea extracts of cellular monolayers was isolated with either anti-fibronectin coupled to Protein A-Sepharose or double antibody immunoprecipitation and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Sepharose 181-190 fibronectin 1 Homo sapiens 16-27 567240-1 1978 Fibronectin, a fibroblast surface protein, was purified from human and chicken plasma and extracts of cultured chicken fibroblasts with affinity chromatography on gelatin coupled to Sepharose particles. Sepharose 182-191 fibronectin 1 Homo sapiens 0-11 78710-0 1978 Separation and characterization of two bovine alpha1-fetoprotein molecular variants by concanavalin A-Sepharose chromatography. Sepharose 102-111 alpha fetoprotein Bos taurus 46-64 207344-11 1978 However, in the lipoproteins apoA-II, which contains lysine but no arginine, was cleaved more rapidly and extensively by agarose-trypsin than apoA-I. Sepharose 121-128 apolipoprotein A2 Homo sapiens 29-36 207344-11 1978 However, in the lipoproteins apoA-II, which contains lysine but no arginine, was cleaved more rapidly and extensively by agarose-trypsin than apoA-I. Sepharose 121-128 apolipoprotein A1 Homo sapiens 29-35 664901-1 1978 The polymorphism of properdin factor B (Bf, C3 proactivator) in a population sample from Hessen, Germany has been investigated by agarose gel electrophoresis and immunofixation. Sepharose 130-137 complement factor B Homo sapiens 20-38 664901-1 1978 The polymorphism of properdin factor B (Bf, C3 proactivator) in a population sample from Hessen, Germany has been investigated by agarose gel electrophoresis and immunofixation. Sepharose 130-137 complement factor B Homo sapiens 40-59 208775-2 1978 Then, in order to isolate gonadotrophs, cyanogen bromide-activated Sepharose whichwas conjugated with LHRH (LHRH-Sepharose) was added to the culture medium. Sepharose 67-76 gonadotropin releasing hormone 1 Homo sapiens 102-106 208775-2 1978 Then, in order to isolate gonadotrophs, cyanogen bromide-activated Sepharose whichwas conjugated with LHRH (LHRH-Sepharose) was added to the culture medium. Sepharose 67-76 gonadotropin releasing hormone 1 Homo sapiens 108-122 208775-7 1978 These results demonstrate that LHRH conjugated to Sepharose can be used to separate gonadotrophs from other 2A8 cells, and it is suggested that the hypertrophy of some cells might be due to persistent stimulation by LHRH which is likely bound to receptors on the cell membrane. Sepharose 50-59 gonadotropin releasing hormone 1 Homo sapiens 31-35 656443-0 1978 Separation of human renal renin and pseudorenin by affinity chromatography on hemoglobin-Sepharose-2B. Sepharose 89-98 renin Homo sapiens 26-31 656443-1 1978 Human renal renin (EC 3.4.99.19) and pseudorenin were easily separated in a single step by affinity chromatography on hemoglobin-Sepharose-2B. Sepharose 129-138 renin Homo sapiens 12-17 569372-1 1978 Bovine thrombin was insolubilized by attachment to cyanogen bromide-activated Sepharose (Sepharose-thrombin) or to activated (Affi-Gel 10) agarose containing a 10 A long arm (Affi-Gel-thrombin). Sepharose 78-87 coagulation factor II, thrombin Bos taurus 7-15 569372-1 1978 Bovine thrombin was insolubilized by attachment to cyanogen bromide-activated Sepharose (Sepharose-thrombin) or to activated (Affi-Gel 10) agarose containing a 10 A long arm (Affi-Gel-thrombin). Sepharose 139-146 coagulation factor II, thrombin Bos taurus 7-15 569372-3 1978 The thrombin beads hydrolyzed the synthetic tripeptide Bz-Phe-Val-Arg-pNA (S-2160) at different rates, with the Sepharose-thrombin more active (220 esterase units per ml) than Affi-Gel thrombin (20.4 units per ml). Sepharose 112-121 coagulation factor II, thrombin Bos taurus 4-12 569372-3 1978 The thrombin beads hydrolyzed the synthetic tripeptide Bz-Phe-Val-Arg-pNA (S-2160) at different rates, with the Sepharose-thrombin more active (220 esterase units per ml) than Affi-Gel thrombin (20.4 units per ml). Sepharose 112-121 coagulation factor II, thrombin Bos taurus 122-130 569372-3 1978 The thrombin beads hydrolyzed the synthetic tripeptide Bz-Phe-Val-Arg-pNA (S-2160) at different rates, with the Sepharose-thrombin more active (220 esterase units per ml) than Affi-Gel thrombin (20.4 units per ml). Sepharose 112-121 coagulation factor II, thrombin Bos taurus 122-130 79230-1 1978 An assay of human antiplasmins has been developed utilizing radial diffusion of plasma from wells cut in plasmin-enriched, fibrinogen-agarose plates. Sepharose 134-141 fibrinogen beta chain Homo sapiens 123-133 276877-3 1978 In the absence of membranes a presumably unglycosylated form of alpha-lactalbumin was synthesized that bound neither to concanavalin A-Sepharose nor to Ricinus communis lectin-agarose and that contained an amino-terminal signal peptide region comprising 19 amino acid residues. Sepharose 135-144 lactalbumin, alpha Rattus norvegicus 64-81 276877-3 1978 In the absence of membranes a presumably unglycosylated form of alpha-lactalbumin was synthesized that bound neither to concanavalin A-Sepharose nor to Ricinus communis lectin-agarose and that contained an amino-terminal signal peptide region comprising 19 amino acid residues. Sepharose 176-183 lactalbumin, alpha Rattus norvegicus 64-81 247990-2 1978 Peptide-elongation factors were purified from rat liver and treated with cholesterol esterase and phospholipase A2 immobilized on Sepharose 4B. Sepharose 130-139 phospholipase A2 group IB Rattus norvegicus 98-114 632297-3 1978 After an initial ion exchange chromatography step on sulfopropyl (SP)-Sephadex at pH 2.6, cationic trypsinogen was separated from the majority of trypsin activity by passage through an affinity column of lima bean trypsin inhibitor-agarose at high ionic strength. Sepharose 232-239 serine protease 1 Homo sapiens 90-110 75886-1 1978 alpha-Fetoprotein has been prepared from human fetal tissue by procedures utilizing DEAE-Sephadex, concanavalin A-Sepharose, and isoelectric focusing. Sepharose 114-123 alpha fetoprotein Homo sapiens 0-17 566118-2 1978 It was that gel filtration chromatography on agarose columns can be used selectively to purify lysozyme, due to the fact that this protein interacts with the agarose matrix and elutes later than the corresponding total volume for the column. Sepharose 45-52 lysozyme Homo sapiens 95-103 566118-2 1978 It was that gel filtration chromatography on agarose columns can be used selectively to purify lysozyme, due to the fact that this protein interacts with the agarose matrix and elutes later than the corresponding total volume for the column. Sepharose 158-165 lysozyme Homo sapiens 95-103 363599-5 1978 Gel filtration on Sephadex G-200 and affinity chromatography on Concanvalin A-Sepharose have shown that antigen-dependent MIF is a glycoprotein of approximately 70,000 molecular weight (Lowe & Lachmann, 1974). Sepharose 78-87 macrophage migration inhibitory factor Ovis aries 122-125 363599-7 1978 Antigen-dependent MIF was also purified by affinity chromatography on PPD-Sepharose. Sepharose 74-83 macrophage migration inhibitory factor Ovis aries 18-21 205865-5 1978 Further analysis of the EGF/URO-labeled components by affinity chromatography on concanavalin A-Sepharose, by disc gel electrophoresis, and by enzymatic digestion suggests that the major specific binding component for EGF/URO in liver membranes is a glycoprotein subunit of approximately 100,000 daltons that possesses a 20,000-dalton portion inaccessible to proteolytic cleavage when the subunit is anchored in the membrane. Sepharose 96-105 epidermal growth factor Rattus norvegicus 24-27 655629-4 1978 The products (alpha, beta1 and gamma1) of the common alleles at the three ADH loci (ADH1 ADH2 and ADH3 respectively) were found to show slight, but significant differences in their affinities for Blue Sepharose. Sepharose 201-210 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 21-38 655629-4 1978 The products (alpha, beta1 and gamma1) of the common alleles at the three ADH loci (ADH1 ADH2 and ADH3 respectively) were found to show slight, but significant differences in their affinities for Blue Sepharose. Sepharose 201-210 alcohol dehydrogenase 1A (class I), alpha polypeptide Homo sapiens 74-77 655629-4 1978 The products (alpha, beta1 and gamma1) of the common alleles at the three ADH loci (ADH1 ADH2 and ADH3 respectively) were found to show slight, but significant differences in their affinities for Blue Sepharose. Sepharose 201-210 alcohol dehydrogenase 1A (class I), alpha polypeptide Homo sapiens 84-88 655629-4 1978 The products (alpha, beta1 and gamma1) of the common alleles at the three ADH loci (ADH1 ADH2 and ADH3 respectively) were found to show slight, but significant differences in their affinities for Blue Sepharose. Sepharose 201-210 alcohol dehydrogenase 1B (class I), beta polypeptide Homo sapiens 89-93 655629-4 1978 The products (alpha, beta1 and gamma1) of the common alleles at the three ADH loci (ADH1 ADH2 and ADH3 respectively) were found to show slight, but significant differences in their affinities for Blue Sepharose. Sepharose 201-210 alcohol dehydrogenase 1C (class I), gamma polypeptide Homo sapiens 98-102 655629-8 1978 The products (gamma1 and gamma2) of the common alleles (ADH31 and ADH32 respectively) at the ADH3 locus showed a pronounced difference in their affinities: the gamma1gamma1 isozyme was firmly adsorbed by Blue Sepharose, whereas the gamma2gamma2 isozyme was not adsorbed. Sepharose 209-218 alcohol dehydrogenase 1C (class I), gamma polypeptide Homo sapiens 56-60 655629-11 1978 The "usual" and "atypical" forms of ADH were indistinguishable by Blue Sepharose column chromatography. Sepharose 71-80 alcohol dehydrogenase 1A (class I), alpha polypeptide Homo sapiens 36-39 307001-10 1978 Gel filtration, polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, and affinity chromatography with concanavalin A-Sepharose indicated that M-CSF from yolk sacs was a glycoprotein with an apparent molecular weight of 60,000. Sepharose 142-151 colony stimulating factor 1 (macrophage) Mus musculus 167-172 95912-1 1978 A subunit of succinylated (40:1 molar ratio, succinic anhydride:lysine residues) human thyroglobulin (Tg) was prepared by gel filtration on 6% and 4% agarose. Sepharose 150-157 thyroglobulin Homo sapiens 87-100 24475-2 1978 D-Amino acid oxidase (D-amino acid: oxygen oxidoreductase (deaminating), EC 1.4.3.3) apoenzyme, holoenzyme and the enzyme-benzoate complex were active and stable when immobilized to aminoalkyl or carboxyalkyl agarose, or to cyanogen bromide-activated agarose. Sepharose 209-216 D-amino acid oxidase Homo sapiens 0-20 25241-7 1978 These derivatives may be covalently linked to Sepharose providing an insolubilized form of CSF to study interactions of CSF with the cell surface. Sepharose 46-55 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 91-94 25241-7 1978 These derivatives may be covalently linked to Sepharose providing an insolubilized form of CSF to study interactions of CSF with the cell surface. Sepharose 46-55 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 120-123 632582-4 1978 The mobilities of these components changed little on reduction, which suggested that they represented single polypeptide chains, An identical pattern was obtained with Sepharose-linked Fc fragments of human IgG1, but neither Fab fragments of IgG1 nor IgM appeared to bind these components. Sepharose 168-177 LOC105243590 Mus musculus 207-211 580493-8 1978 In addition, the catalytic properties of alpha-thrombin covalently attached to agarose gel beads were also examined. Sepharose 79-86 coagulation factor II, thrombin Bos taurus 47-55 564210-0 1978 The separation of the myosin light chains on agarose beads. Sepharose 45-52 myosin heavy chain 14 Homo sapiens 22-28 204321-6 1978 The purified CEA pools also showed quantitative variations in the binding profiles on Con A-Sepharose. Sepharose 92-101 CEA cell adhesion molecule 3 Homo sapiens 13-16 564210-1 1978 The myosin light chains from rabbit skeletal muscle and bovine and human hearts are separated according to their molecular weights by filtration on agarose beads. Sepharose 148-155 myosin heavy chain 14 Homo sapiens 4-10 75238-12 1978 Second, C1s released by the activation can be quantitated by single radial diffusion if the agarose contains high concentrations of anti-C1q to confine C1, also containing C1s, to the area near the application well, and lesser concentrations of anti-C1s to permit free C1s to produce a measurable ring. Sepharose 92-99 complement C1s Homo sapiens 8-11 75238-12 1978 Second, C1s released by the activation can be quantitated by single radial diffusion if the agarose contains high concentrations of anti-C1q to confine C1, also containing C1s, to the area near the application well, and lesser concentrations of anti-C1s to permit free C1s to produce a measurable ring. Sepharose 92-99 complement C1q A chain Homo sapiens 137-140 75564-6 1978 These results indicate that the removal of antithrombin III is the major problem in the purification of H-TGL and LPL from human post-heparin plasma by heparin-Sepharose affinity chromatography. Sepharose 160-169 serpin family C member 1 Homo sapiens 43-59 621432-2 1978 PF-4 was iodinated, repurified by affinity chromatography on heparin-Sepharose, and incubated with rabbit antiserum and a source of unlabeled antigen. Sepharose 69-78 platelet factor 4 Homo sapiens 0-4 273258-1 1978 Two electrophoretic variants of murine complement component 3 (C3) were detected by using high-voltage electrophoresis of fresh mouse serum in agarose gels. Sepharose 143-150 complement component 3 Mus musculus 63-65 75564-6 1978 These results indicate that the removal of antithrombin III is the major problem in the purification of H-TGL and LPL from human post-heparin plasma by heparin-Sepharose affinity chromatography. Sepharose 160-169 lipoprotein lipase Homo sapiens 114-117 83759-2 1978 A monospecific immunoglobulin fraction, covalently coupled to Sepharose 4B, was used as binding agent and the elution conditions for PZP are described. Sepharose 62-74 PZP alpha-2-macroglobulin like Homo sapiens 133-136 620051-9 1978 Photooxidized fibrinogen has affinity for thrombin-activated fibrinogen-Sepharose and thrombin-activated fragment N-DSK-Sepharose. Sepharose 72-81 fibrinogen beta chain Homo sapiens 14-24 620051-9 1978 Photooxidized fibrinogen has affinity for thrombin-activated fibrinogen-Sepharose and thrombin-activated fragment N-DSK-Sepharose. Sepharose 72-81 coagulation factor II, thrombin Homo sapiens 42-50 620051-9 1978 Photooxidized fibrinogen has affinity for thrombin-activated fibrinogen-Sepharose and thrombin-activated fragment N-DSK-Sepharose. Sepharose 72-81 fibrinogen beta chain Homo sapiens 61-71 620051-9 1978 Photooxidized fibrinogen has affinity for thrombin-activated fibrinogen-Sepharose and thrombin-activated fragment N-DSK-Sepharose. Sepharose 120-129 fibrinogen beta chain Homo sapiens 14-24 620051-9 1978 Photooxidized fibrinogen has affinity for thrombin-activated fibrinogen-Sepharose and thrombin-activated fragment N-DSK-Sepharose. Sepharose 120-129 coagulation factor II, thrombin Homo sapiens 86-94 73414-2 1978 The technique is based entirely on the physicochemical properties of the alpha1-fetoprotein molecule and uses sequential purification steps: ion-exchange chromatography on DEAE-Sephadex A-50, molecular-sieve chromatography on Sephadex G-200, negative-affinity chromatography on Sepharose-Blue Dextran, positivepaffinity chromatography on concanavalin A-Sepharose and, finally, molecular-sieve chromatography on Sephadex G-100. Sepharose 278-287 alpha fetoprotein Homo sapiens 73-91 318380-2 1978 Glucose-6-phosphate dehydrogenase (G6PDH) has been purified to homogeneity from rat and chick brain by affinity chromatography with Sepharose bound 2",5" ADP. Sepharose 132-141 glucose-6-phosphate dehydrogenase Rattus norvegicus 0-33 318380-2 1978 Glucose-6-phosphate dehydrogenase (G6PDH) has been purified to homogeneity from rat and chick brain by affinity chromatography with Sepharose bound 2",5" ADP. Sepharose 132-141 glucose-6-phosphate dehydrogenase Rattus norvegicus 35-40 217588-2 1978 The solid phase immunoabsorbent was prepared by attaching LHRH to Sepharose via an RNase spacer. Sepharose 66-75 gonadotropin releasing hormone 1 Rattus norvegicus 58-62 720954-5 1978 The isolated IgG fraction insolubilised with CNBr-Sepharose 4B retained the ability to complex with F VIII. Sepharose 50-59 coagulation factor VIII Homo sapiens 100-106 308033-1 1978 A group of 202 unrelated Italians were screened for alpha1-antitrypsin using agarose-acrylamide electrophoresis and isoelectric focusing. Sepharose 77-84 serpin family A member 1 Homo sapiens 52-70 338662-1 1978 This paper reports a solid-phase sandwich technique for the assay of human IgE using anti-IgE antibodies bound to magnetic polyacrylamide-agarose beads. Sepharose 138-145 immunoglobulin heavy constant epsilon Homo sapiens 75-78 749809-5 1978 Using alpha-hydroxy-isocaproyl-tyrosine covalently coupled to Sepharose 4B as a bioadsorbent; the form 2 of acylase from human liver was isolated and separated from the form 1, aminoacylase and aspartyl acylase. Sepharose 62-71 aminoacylase 1 Homo sapiens 108-115 338662-1 1978 This paper reports a solid-phase sandwich technique for the assay of human IgE using anti-IgE antibodies bound to magnetic polyacrylamide-agarose beads. Sepharose 138-145 immunoglobulin heavy constant epsilon Homo sapiens 90-93 338662-4 1978 The use of magnetic polyacrylamide-agarose beads and of enzyme-labeled antibodies makes the measurement of IgE in human sera easy to perform, safe, and reproducible. Sepharose 35-42 immunoglobulin heavy constant epsilon Homo sapiens 107-110 27163-0 1978 Stabilization of human fibroblast interferon purified on concanavalin A-agarose. Sepharose 72-79 interferon beta 1 Homo sapiens 23-44 359714-0 1978 Quantitative immunofixation of proteins following zone electrophoresis in agarose gel: application to the determination of the stoichiometry of the alpha1-antitrypsin-elastase interaction. Sepharose 74-81 serpin family A member 1 Homo sapiens 148-166 27163-1 1978 Human fibroblast interferon, obtained by chromatography on concanavalin A-agarose, was stable for at least a month in 30--50 per cent ethylene glycol at 4 degrees, --20 degrees, and --70 degrees C. The succinct point of the present finding is that human fibroblast interferon may be stabilized by ethylene glycol alone without the addition of bovine serum albumin and "back-contamination" of the interferon preparation. Sepharose 74-81 interferon beta 1 Homo sapiens 6-27 27562-3 1978 The observation that LIF activity is reduced when the ambient pH falls below 7.2 is suggested as an explanation firstly for the "escape" phenomenon seen particularly in capillary tube assays for LIF, and secondly for the reduced sensitivity of the capillarly tube assay in comparison with the corresponding agarose plate assay. Sepharose 307-314 LIF interleukin 6 family cytokine Homo sapiens 21-24 32437-1 1978 The NADH: (acceptor) oxidoreductase (EC 1.6.99.3) was isolated from human erythrocyte ghosts by a procedure including Triton X-100 solubilization, affinity chromatography on an NAD+-Sepharose 4B column, ammonium sulfate precipitation, and isoelectric focusing. Sepharose 182-191 thioredoxin reductase 1 Homo sapiens 21-35 82589-1 1978 A method for preparation of alpha-2 macroglobulin (alpha2M) by immunoadsorbent chromatography utilizing rabbit--anti-human alpha2M-conjugated Sepharose 4B is described. Sepharose 142-151 alpha-2-macroglobulin Homo sapiens 28-49 82589-1 1978 A method for preparation of alpha-2 macroglobulin (alpha2M) by immunoadsorbent chromatography utilizing rabbit--anti-human alpha2M-conjugated Sepharose 4B is described. Sepharose 142-151 alpha-2-macroglobulin Homo sapiens 51-58 82589-1 1978 A method for preparation of alpha-2 macroglobulin (alpha2M) by immunoadsorbent chromatography utilizing rabbit--anti-human alpha2M-conjugated Sepharose 4B is described. Sepharose 142-151 alpha-2-macroglobulin Homo sapiens 123-130 714880-0 1978 Purification fo cathepsin D by AH-sepharose affinity chromatography. Sepharose 34-43 cathepsin D Rattus norvegicus 16-27 24840-1 1978 Highly purified glutamine synthetase has been isolated from Chlorella and immobilized on BrCN-sepharose. Sepharose 94-103 glutamate-ammonia ligase Homo sapiens 16-36 725535-1 1978 Antibodies against partially purified human leucocyte interferon (PIF) were bound to Sepharose 4B and crude interferons applied on this affinity column were purified, up to 8 x 10(5) interferon units (IFU) per mg protein in one step. Sepharose 85-94 PIF1 5'-to-3' DNA helicase Homo sapiens 44-70 725535-2 1978 Antibodies against PIF were absorbed with immobilized crude human leucocyte interferon bound to Sepharose, whereby antibodies against impurities were predominantly removed. Sepharose 96-105 PIF1 5'-to-3' DNA helicase Homo sapiens 19-22 334799-5 1977 Elastase and cathepsin G were purified from human neutrophils by Trasylol-Sepharose and CM-cellulose chromatography. Sepharose 74-83 cathepsin G Homo sapiens 13-24 588589-2 1977 Intrinsic factor or cobalophilin were removed by incubating human gastric juice and pig pyloric extract with purified anti-intrinsic factor and anti-cobalophilin immunoglobulin-G, respectively, covalently coupled to Sepharose. Sepharose 216-225 cobalamin binding intrinsic factor Homo sapiens 0-16 588589-4 1977 The one remaining semipurified vitamin B-12-binding protein (intrinsic factor, cobalophilin or transcobalamin II) was then isolated by vitamin B-12-Sepharose affinity chromatography. Sepharose 148-157 cobalamin binding intrinsic factor Homo sapiens 61-77 414742-3 1977 The enzyme was purified by using (NH4)2SO4 precipitation and affinity chromatography on an alpha-lactalbumin-Sepharose column. Sepharose 109-118 lactalbumin alpha Homo sapiens 91-108 353657-5 1977 A purified antithrombin III, prepared by affinity chromatography, contained an immunoreactive material of higher molecular size which has no activity and a higher mobility in agarose gel. Sepharose 175-182 serpin family C member 1 Homo sapiens 11-27 353657-0 1977 [Electrophoretic mobility of antithrombin III in an agarose gel with heparin. Sepharose 52-59 serpin family C member 1 Homo sapiens 29-45 353657-2 1977 The electrophoretic mobility of several forms of antithrombin III in an agarose gel has been compared with the mobility in a gel containing heparin. Sepharose 72-79 serpin family C member 1 Homo sapiens 49-65 21704-2 1977 Affinity chromatographic purification of the lectins using bovine submaxillary gland mucin-conjugated Sepharose resulted in the separation of the lectins into four fractions; one major and three minor lectins. Sepharose 102-111 mucin 1, cell surface associated Bos taurus 85-90 144600-2 1977 Phosphofructokinase to prepared is partially phosphorylated and may be fractioned into three distinct species with sedimentation coefficients of 30 S, 18 S and 13 S by chromatography of agarose gels, hydroxyapatite or DEAE-cellulose. Sepharose 186-193 ATP-dependent 6-phosphofructokinase, muscle type Oryctolagus cuniculus 0-19 270721-0 1977 Estradiol receptor of calf uterus: interactions with heparin-agarose and purification. Sepharose 61-68 estrogen receptor 1 Bos taurus 0-18 270721-1 1977 Heparin attached covalently to agarose beads binds the "native" form of the estradiol receptor with very high affinity. Sepharose 31-38 estrogen receptor 1 Bos taurus 76-94 911763-2 1977 When coupled to Sepharose as an immunoadsorbent, they are capable of resolving subfragment 1, heavy meromyosin, and myosin into two fractions, one rich in alkali 1 and the other rich in alkali 2. Sepharose 16-25 myosin, heavy chain 15 Gallus gallus 104-110 924364-0 1977 Complex formation of crosslinked fibrin oligomers with agarose-coupled fibrinogen and fibrin. Sepharose 55-62 fibrinogen beta chain Homo sapiens 71-81 924364-4 1977 These fractions were subjected to affinity chromatography on agarose-coupled fibrinogen and fibrin. Sepharose 61-68 fibrinogen beta chain Homo sapiens 77-87 408379-1 1977 When Factor VIII/von Willebrand factor (FVIII/vWF) protein is rechromatographed on 4% agarose in 0.25 M CaCl(2), the protein and vWF activity appear in the void volume, but most of the FVIII procoagulant activity elutes later. Sepharose 86-93 von Willebrand factor Homo sapiens 17-38 408379-1 1977 When Factor VIII/von Willebrand factor (FVIII/vWF) protein is rechromatographed on 4% agarose in 0.25 M CaCl(2), the protein and vWF activity appear in the void volume, but most of the FVIII procoagulant activity elutes later. Sepharose 86-93 coagulation factor VIII Homo sapiens 40-45 408379-1 1977 When Factor VIII/von Willebrand factor (FVIII/vWF) protein is rechromatographed on 4% agarose in 0.25 M CaCl(2), the protein and vWF activity appear in the void volume, but most of the FVIII procoagulant activity elutes later. Sepharose 86-93 von Willebrand factor Homo sapiens 46-49 408379-1 1977 When Factor VIII/von Willebrand factor (FVIII/vWF) protein is rechromatographed on 4% agarose in 0.25 M CaCl(2), the protein and vWF activity appear in the void volume, but most of the FVIII procoagulant activity elutes later. Sepharose 86-93 von Willebrand factor Homo sapiens 129-132 408379-2 1977 Recent evidence suggests that the delayed FVIII procoagulant activity is a proteolytically modified form of FVIII/vWF protein that filters anomalously from agarose in 0.25 M CaCl(2). Sepharose 156-163 coagulation factor VIII Homo sapiens 42-47 408379-2 1977 Recent evidence suggests that the delayed FVIII procoagulant activity is a proteolytically modified form of FVIII/vWF protein that filters anomalously from agarose in 0.25 M CaCl(2). Sepharose 156-163 coagulation factor VIII Homo sapiens 108-113 408379-2 1977 Recent evidence suggests that the delayed FVIII procoagulant activity is a proteolytically modified form of FVIII/vWF protein that filters anomalously from agarose in 0.25 M CaCl(2). Sepharose 156-163 von Willebrand factor Homo sapiens 114-117 270704-0 1977 Erythropoietin: isolation by affinity chromatography with lectin-agarose derivatives. Sepharose 65-72 erythropoietin Homo sapiens 0-14 270704-1 1977 Affinity chromatography using agarose-bound lectins was used to isolate erythropoietin from crude preparations of sheep plasma and human urinary erythropoietin. Sepharose 30-37 erythropoietin Ovis aries 72-86 270704-1 1977 Affinity chromatography using agarose-bound lectins was used to isolate erythropoietin from crude preparations of sheep plasma and human urinary erythropoietin. Sepharose 30-37 erythropoietin Homo sapiens 145-159 270704-3 1977 Only wheat germ agglutinin-agarose and phytohemagglutinin-agarose derivatives had significant affinity for erythropoietin. Sepharose 27-34 erythropoietin Homo sapiens 107-121 270704-3 1977 Only wheat germ agglutinin-agarose and phytohemagglutinin-agarose derivatives had significant affinity for erythropoietin. Sepharose 58-65 erythropoietin Homo sapiens 107-121 270704-4 1977 By use of wheat germ aggutinin-agarose columns, erythropoietin could be separated from over 95% of the initial starting protein, resulting in an 8-to 100-fold purification and a recovery of at least 40% depending on the source of the hormone. Sepharose 31-38 erythropoietin Homo sapiens 48-62 270704-5 1977 Affinity chromatography with agarose-bound lectins provides a simple rapid method for isolating erythropoietin from crude preparations of the hormone. Sepharose 29-36 erythropoietin Homo sapiens 96-110 330529-2 1977 The scheme is based on an initial separation of thioredoxin from the two reductases by affinity chromatography on agarose-bound N6-(6-aminohexyl)-adenosine 2",5"-bisphosphate (agarose-2",5"-ADP). Sepharose 114-121 thioredoxin Homo sapiens 48-59 330529-4 1977 Thioredoxin was purified to homogeneity by immunoadsorption to agarose containing immobilized goat anti-thioredoxin. Sepharose 63-70 thioredoxin Homo sapiens 0-11 893399-0 1977 Purification of 3-hydroxy-3-methylglutaryl coenzyme A reductase by affinity chromatography on blue dextran/Sepharose 4B. Sepharose 107-116 3-hydroxy-3-methylglutaryl-CoA reductase Rattus norvegicus 16-63 69494-4 1977 TSTA was well separated from mouse histocompatibility antigen H-2 by a sequence of procedures, including gel filtration, lectin affinity chromatography, column electrophoresis, and rechromatography on agarose, showed only three major bands on polyacrylamide gel electrophoresis. Sepharose 201-208 heat shock protein 90, alpha (cytosolic), class A member 1 Mus musculus 0-4 560374-1 1977 A beta-galactoside-specific lectin, capable of agglutinating trypsinized rabbit erythrocytes, was isolated from 13-day-old embryonic chick thigh muscle and purified 1000-fold by affinity chromatography on asialofetuin/Sepharose and Sephadex G-100. Sepharose 218-227 galectin 3 Gallus gallus 28-34 884004-4 1977 Post heparin plasma lipoprotein lipase activity isolated and partially purified by heparin Sepharose affinity chromatography was determined quantitatively. Sepharose 91-100 lipoprotein lipase Homo sapiens 20-38 332508-6 1977 Also, whereas essentially all of the HLA-A and B antigens and about half of the HLA-CW2 antigen were adsorbed strongly by Lens culinaris lectin-Sepharose, the remaining HLA-CW2 antigen was bound much more weakly and did not require sugar for elution. Sepharose 144-153 major histocompatibility complex, class I, B Homo sapiens 37-48 903179-6 1977 Preparative affinity chromatography of plasma on gelatin coupled to Sepharose gave electrophoretically and immunologically pure fibronectin in high yields. Sepharose 68-77 fibronectin 1 Homo sapiens 128-139 18669-3 1977 Studies on aminolaevulate dehydratase attached to Sepharose. Sepharose 50-59 aminolevulinate dehydratase Bos taurus 11-37 18669-4 1977 Bovine liver aminolaevulate dehydratase (ALA-D) has been chemically attached to Sepharose 4B and its properties have been studied. Sepharose 80-92 aminolevulinate dehydratase Bos taurus 13-39 18669-4 1977 Bovine liver aminolaevulate dehydratase (ALA-D) has been chemically attached to Sepharose 4B and its properties have been studied. Sepharose 80-92 aminolevulinate dehydratase Bos taurus 41-46 408067-5 1977 Evidence is presented that immunodiffusion values of properdin might be affected by precipitation of a C3-properdin complex in gels containing Mg2+ ions after the activation of the properdin system by agarose. Sepharose 201-208 complement factor properdin Homo sapiens 106-115 408067-5 1977 Evidence is presented that immunodiffusion values of properdin might be affected by precipitation of a C3-properdin complex in gels containing Mg2+ ions after the activation of the properdin system by agarose. Sepharose 201-208 complement factor properdin Homo sapiens 106-115 924589-0 1977 Estimation of serum haptoglobin levels by using agarose gel electrophoresis. Sepharose 48-55 haptoglobin Homo sapiens 20-31 894146-3 1977 Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme activities, respectively. Sepharose 46-55 lipase C, hepatic type Homo sapiens 29-34 69721-9 1977 Moreover, P60 from cytoplasmic extracts was retained on single-stranded DNA-Sepharose columns, demonstrating that cellular P60 binds to DNA. Sepharose 76-85 interferon induced protein with tetratricopeptide repeats 3 Homo sapiens 10-13 69721-9 1977 Moreover, P60 from cytoplasmic extracts was retained on single-stranded DNA-Sepharose columns, demonstrating that cellular P60 binds to DNA. Sepharose 76-85 interferon induced protein with tetratricopeptide repeats 3 Homo sapiens 123-126 70787-7 1977 Furthermore, when C2 is bound to C4b-Sepharose and then reacted with C1s, only the C2a fragment is released from the solid phase C2-C4b-Sepharose into the fluid phase, and the C2b fragment remains noncovalently bound to C4b-Sepharose. Sepharose 37-46 complement C4B (Chido blood group) Homo sapiens 33-36 70787-7 1977 Furthermore, when C2 is bound to C4b-Sepharose and then reacted with C1s, only the C2a fragment is released from the solid phase C2-C4b-Sepharose into the fluid phase, and the C2b fragment remains noncovalently bound to C4b-Sepharose. Sepharose 37-46 secretoglobin family 2B member 3, pseudogene Homo sapiens 176-179 70787-7 1977 Furthermore, when C2 is bound to C4b-Sepharose and then reacted with C1s, only the C2a fragment is released from the solid phase C2-C4b-Sepharose into the fluid phase, and the C2b fragment remains noncovalently bound to C4b-Sepharose. Sepharose 136-145 complement C4B (Chido blood group) Homo sapiens 33-36 70787-7 1977 Furthermore, when C2 is bound to C4b-Sepharose and then reacted with C1s, only the C2a fragment is released from the solid phase C2-C4b-Sepharose into the fluid phase, and the C2b fragment remains noncovalently bound to C4b-Sepharose. Sepharose 136-145 complement C1s Homo sapiens 69-72 70787-7 1977 Furthermore, when C2 is bound to C4b-Sepharose and then reacted with C1s, only the C2a fragment is released from the solid phase C2-C4b-Sepharose into the fluid phase, and the C2b fragment remains noncovalently bound to C4b-Sepharose. Sepharose 136-145 complement C4B (Chido blood group) Homo sapiens 33-36 70787-7 1977 Furthermore, when C2 is bound to C4b-Sepharose and then reacted with C1s, only the C2a fragment is released from the solid phase C2-C4b-Sepharose into the fluid phase, and the C2b fragment remains noncovalently bound to C4b-Sepharose. Sepharose 136-145 complement C1s Homo sapiens 69-72 194848-1 1977 A low molecular weight, highly basic DNA-binding protein was purified from several oncornaviruses by the sequential procedures of gel filtration in guanidine-hydrochloride, DEAE-cellulose chromatography and affinity chromatography on single-stranded DNA sepharose. Sepharose 254-263 heat shock transcription factor 4 Mus musculus 37-56 560966-4 1977 Pig heart lipoamide dehydrogenase (NADH: lipoamide oxidoreductase, EC 1.6.4.3) has been immobilised to Sepharose by thiol-disulphide interchange via a series of thiolated spacer molecules of increasing length. Sepharose 103-112 dihydrolipoamide dehydrogenase Sus scrofa 10-33 195760-1 1977 The interaction between the lipoprotein carrying the Lp(a) antigen, i.e. the Lp(a) lipoprotein, and agarose gels substituted with glycosaminoglycans, as well as the precipitation of the Lp(a) lipoprotein by Ca++ were studied. Sepharose 100-107 lipoprotein(a) Homo sapiens 53-58 896485-1 1977 Alpha-lactalbumin messenger RNA was partially purified from RNA extracted from 3-5 day lactating rat mammary glands on a poly(U)-sepharose column followed by sucrose gradient centrifugation. Sepharose 129-138 lactalbumin, alpha Rattus norvegicus 0-17 333666-2 1977 Separation of free and bound steroid was effected by Sepharose-coupled antiprogesterone-11alpha-hemisuccinyl bovine serum albumin antiserum (Sepharose-antisera). Sepharose 53-62 albumin Homo sapiens 116-129 861248-0 1977 The interaction of nonhistone chromosomal proteins HMG1 and HMG2 with subfractions of H1 histone immobilized on agarose. Sepharose 112-119 high mobility group box 1 Bos taurus 51-55 861248-0 1977 The interaction of nonhistone chromosomal proteins HMG1 and HMG2 with subfractions of H1 histone immobilized on agarose. Sepharose 112-119 high mobility group protein B2 Bos taurus 60-64 861248-1 1977 Chromatographically isolated subfractions of calf thymus H1 histone have been covalently coupled to agarose beads and tested for their ability to form complexes with the non-histone proteins HMG1 and HMG2 (High Mobility Group proteins, Walker, J.M., Goodwin, G.H. Sepharose 100-107 high mobility group box 1 Bos taurus 191-195 861248-1 1977 Chromatographically isolated subfractions of calf thymus H1 histone have been covalently coupled to agarose beads and tested for their ability to form complexes with the non-histone proteins HMG1 and HMG2 (High Mobility Group proteins, Walker, J.M., Goodwin, G.H. Sepharose 100-107 high mobility group protein B2 Bos taurus 200-204 861248-6 1977 When a mixture of HMG1 and HMG2 is passed through a column of H1 histone-agarose, the HMG2 does not bind. Sepharose 73-80 high mobility group box 1 Bos taurus 18-22 861248-6 1977 When a mixture of HMG1 and HMG2 is passed through a column of H1 histone-agarose, the HMG2 does not bind. Sepharose 73-80 high mobility group protein B2 Bos taurus 27-31 861248-6 1977 When a mixture of HMG1 and HMG2 is passed through a column of H1 histone-agarose, the HMG2 does not bind. Sepharose 73-80 high mobility group protein B2 Bos taurus 86-90 16004-1 1977 Rat liver ornithine decarboxylase induced by injection of thioacetamide has been separated into at least two fractions by covalent chromatography on an activated thiol-Sepharose 4B column. Sepharose 168-177 ornithine decarboxylase 1 Rattus norvegicus 10-33 16012-2 1977 The pressor enzyme renin (EC 3.4.99.19) was isolated in a pure and stable form from hog kidney by affinity chromatography on a pepstatin/agarose gel followed by three additional steps of conventional chromatography. Sepharose 137-144 renin Homo sapiens 19-24 868029-2 1977 This result was confirmed by Sepharose 4 B gel filtration analysis of the ultracentrifuged product, which revealed, in additon to mateial with a molecular weight of more than 1 X 10(6) Daltons, three F VIII-active fractions with smaller molecular weight values. Sepharose 29-38 cytochrome c oxidase subunit 8A Homo sapiens 202-206 849737-1 1977 The solube iodoproteins in a transplantable rat throid tumor (Wollman Line 1-8) were studied after in vivo labeling with 125 I and were partially purified by affinity chromatography on anti-thyroglobulin-AGAROSE. Sepharose 204-211 thyroglobulin Rattus norvegicus 190-203 197061-5 1977 The [14C]lecithin in the washed HDL-Sepharose was shown to be a substrate of the LCAT reaction in vitro. Sepharose 36-45 lecithin-cholesterol acyltransferase Homo sapiens 81-85 323292-3 1977 The tests are based on the inhibition of I125-Clq or I125-monoclonal rheumatoid factor (mRF) binding to an insoluble substrate, IgG-Sepharose. Sepharose 132-141 myelin regulatory factor Mus musculus 88-91 864652-4 1977 Pure antibody against mouse LDH-X was obtained by affinity chromatography of the rabbit anti-mouse LDH-X-IgG on pure mouse LDH-X covalently bound to sepharose. Sepharose 149-158 lactate dehydrogenase C Mus musculus 28-33 864652-4 1977 Pure antibody against mouse LDH-X was obtained by affinity chromatography of the rabbit anti-mouse LDH-X-IgG on pure mouse LDH-X covalently bound to sepharose. Sepharose 149-158 lactate dehydrogenase C Mus musculus 99-104 864652-4 1977 Pure antibody against mouse LDH-X was obtained by affinity chromatography of the rabbit anti-mouse LDH-X-IgG on pure mouse LDH-X covalently bound to sepharose. Sepharose 149-158 lactate dehydrogenase C Mus musculus 99-104 403392-8 1977 The very recent work from the laboratory of Heding, however, has brought about major advances in this area in which human C-peptide and proinsulin can be separated in the plasma by the use of Sepharose particles. Sepharose 192-201 insulin Homo sapiens 122-131 403392-8 1977 The very recent work from the laboratory of Heding, however, has brought about major advances in this area in which human C-peptide and proinsulin can be separated in the plasma by the use of Sepharose particles. Sepharose 192-201 insulin Homo sapiens 136-146 865246-2 1977 Lipoprotein lipase and hepatic triglyceride lipase were differentiated by assay under high and low salt conditions and also by separation on heparin-agarose affinity chromatography columns. Sepharose 149-156 lipoprotein lipase Homo sapiens 0-18 142314-0 1977 The inactivation of thrombin and plasmin by antithrombin III in the presence of sepharose-heparin. Sepharose 80-89 coagulation factor II, thrombin Homo sapiens 20-28 142314-0 1977 The inactivation of thrombin and plasmin by antithrombin III in the presence of sepharose-heparin. Sepharose 80-89 plasminogen Homo sapiens 33-40 142314-0 1977 The inactivation of thrombin and plasmin by antithrombin III in the presence of sepharose-heparin. Sepharose 80-89 serpin family C member 1 Homo sapiens 44-60 139938-4 1977 About 50% of heart cell lipase activity applied to heparin-Sepharose bound to the gel and was eluted with a NaCl gradient. Sepharose 59-68 lipase G, endothelial type Rattus norvegicus 24-30 15612-0 1977 Affinity chromatographic separation of arylsulfatase A and B using Cibacron Blue-Sepharose. Sepharose 81-90 arylsulfatase A Homo sapiens 39-54 403982-1 1977 The immunoprecipitation reaction for the determination of microamounts of alpha1-antitrypsin was conducted in the capillaries filled with a microvolume of a 1% agarose gel (the gel length was 7--8 mm, and the diameter--0.6 mm). Sepharose 160-167 serpin family A member 1 Homo sapiens 74-92 67171-6 1977 GAT-TsF has been partially purified from the crude extract by absorption to GAT-Sepharose and elution with 0.4 to 0.6 KCl. Sepharose 80-89 glycine-N-acyltransferase Mus musculus 0-3 67171-6 1977 GAT-TsF has been partially purified from the crude extract by absorption to GAT-Sepharose and elution with 0.4 to 0.6 KCl. Sepharose 80-89 glycine-N-acyltransferase Mus musculus 76-79 405760-10 1977 Incubation of human serum with either agarose or rat thymocytes resulted in the conversion of factor B, essential for complement activation via the alternative pathway, previously shown to provide the complement activity necessary for the cytotoxic reaction. Sepharose 38-45 distal membrane arm assembly component 2 like Rattus norvegicus 94-102 66068-3 1977 Polyacrylamide slab gel electrophoresis, extended agarose electrophoresis and immunoelectrophoresis demonstrated that mouse hepatoma alpha-fetoprotein migrated at pH 8.6 as a rapid alpha1, or postalbumin globulin. Sepharose 50-57 alpha fetoprotein Mus musculus 133-150 14671-1 1977 (NADPH)-cytochrome P-450 reductase was purified to apparent homogeneity by a procedure utilizing nicotinamide adenine dinucleotide phosphate (NADP)-Sepharose affinity column chromatography. Sepharose 148-157 cytochrome p450 oxidoreductase Rattus norvegicus 1-34 851436-0 1977 Iron transport from Sepharose-bound transferrin. Sepharose 20-29 transferrin Homo sapiens 36-47 402385-11 1977 Furthermore, serum that had been depleted of DBP by treatment with Sepharose containing covalently coupled antibodies against DBP was found to be depleted also of immunoreactivity against anti-GC protein antiserum. Sepharose 67-76 D-box binding PAR bZIP transcription factor Homo sapiens 45-48 402385-11 1977 Furthermore, serum that had been depleted of DBP by treatment with Sepharose containing covalently coupled antibodies against DBP was found to be depleted also of immunoreactivity against anti-GC protein antiserum. Sepharose 67-76 D-box binding PAR bZIP transcription factor Homo sapiens 126-129 405382-5 1977 When the antibody against NADPH-cytochrome c reductase was used, however, immunoadsorption of microsomes on Sepharose-bound antibody produced some separation of NADPH-cytochrome c reductase and cytochrome P-450 from NADH-cytochrome b5 reductase and cytochrome b5. Sepharose 108-117 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 194-210 405382-5 1977 When the antibody against NADPH-cytochrome c reductase was used, however, immunoadsorption of microsomes on Sepharose-bound antibody produced some separation of NADPH-cytochrome c reductase and cytochrome P-450 from NADH-cytochrome b5 reductase and cytochrome b5. Sepharose 108-117 cytochrome b5 type A Rattus norvegicus 221-234 405382-5 1977 When the antibody against NADPH-cytochrome c reductase was used, however, immunoadsorption of microsomes on Sepharose-bound antibody produced some separation of NADPH-cytochrome c reductase and cytochrome P-450 from NADH-cytochrome b5 reductase and cytochrome b5. Sepharose 108-117 cytochrome b5 type A Rattus norvegicus 249-262 845116-4 1977 The catalase was a nondialyzable, cyanide and azide-sensitive, heat-labile protein that coeluted with bovine erythrocyte catalase from Sepharose 6 B. Sepharose 135-144 catalase Bos taurus 4-12 845116-4 1977 The catalase was a nondialyzable, cyanide and azide-sensitive, heat-labile protein that coeluted with bovine erythrocyte catalase from Sepharose 6 B. Sepharose 135-144 catalase Bos taurus 121-129 870819-1 1977 Partially purified ceruloplasmin mRNA was isolated using indirect immunoprecipitation of rat liver polysomes and poly(U)-Sepharose chromatography of polysomal RNA. Sepharose 121-130 ceruloplasmin Rattus norvegicus 19-32 265586-1 1977 Hypoxanthine phosphoribosyltransferase (HPRT, IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) can be purified 5-to 10,000-fold from extracts of HeLa (human) cells by a three-step procedure consisting of high-speed centrifugation, adsorption to Sepharose-conjugated HPRT antibody, and sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Sepharose 253-262 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 0-38 265586-1 1977 Hypoxanthine phosphoribosyltransferase (HPRT, IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) can be purified 5-to 10,000-fold from extracts of HeLa (human) cells by a three-step procedure consisting of high-speed centrifugation, adsorption to Sepharose-conjugated HPRT antibody, and sodium dodecyl sulfate/polyacrylamide gel electrophoresis. Sepharose 253-262 hypoxanthine phosphoribosyltransferase 1 Homo sapiens 40-44 14416-2 1977 The influence of the pH on the separation of high molecular weight derivatives obtained by a limited action of thrombin on fibrinogen was studied by agarose gel chromatography. Sepharose 149-156 coagulation factor II, thrombin Homo sapiens 111-119 14416-2 1977 The influence of the pH on the separation of high molecular weight derivatives obtained by a limited action of thrombin on fibrinogen was studied by agarose gel chromatography. Sepharose 149-156 fibrinogen beta chain Homo sapiens 123-133 14148-0 1977 Interaction of thyroid peroxidase with concanavalin A covalently coupled to agarose. Sepharose 76-83 thyroid peroxidase Homo sapiens 15-33 14148-1 1977 We have investigated the interaction between concanavalin A-agarose (Con A-agarose) and thyroid peroxidase, an integral membrane protein found in the 105,000 X g, 1-h particulate fraction of thyroid tissue. Sepharose 60-67 thyroid peroxidase Homo sapiens 69-106 14148-3 1977 The three types of thyroid peroxidase bind to Con A-agarose and can be eluted with alpha-methyl-D-mannoside. Sepharose 52-59 thyroid peroxidase Homo sapiens 19-37 14148-6 1977 The binding of thyroid peroxidase to Con A-agarose can be inhibited by sugars in the following order: alpha-methyl-D-mannoside greater than D-mannose greater than alpha-methyl-D-glucoside greater than D-glucose greater than D-galactose. Sepharose 43-50 thyroid peroxidase Homo sapiens 15-33 14148-9 1977 Reactivation of the carbohydrate binding site by the addition of Ca2+ and Mn2+ to demetallized Con A-agarose restores thyroid peroxidase binding. Sepharose 101-108 thyroid peroxidase Homo sapiens 118-136 14148-11 1977 In addition, the interaction between thyroid peroxidase and Con A-agarose may provide a new purification tool for thyroid peroxidase. Sepharose 66-73 thyroid peroxidase Homo sapiens 114-132 191025-0 1977 Lysozyme-agarose interaction. Sepharose 9-16 lysozyme Homo sapiens 0-8 265197-0 1977 Electrophoresis of lysozyme into Microscoccus-containing agarose gel: quantitative and analytical applications. Sepharose 57-64 lysozyme Homo sapiens 19-27 265197-1 1977 Electrophoresis of lysozyme into agarose gel containing Micrococcus lysodeikticus causes lysis of the microorganism, allowing the development of two methods, one for quantitation ("lyso-rocket electrophoresis") and the other for electrophoretic characterization ("crossed lyso-electrophoresis") of lysozyme. Sepharose 33-40 lysozyme Homo sapiens 19-27 556956-6 1977 It was confirmed that aminopeptidase was also extracted by Triton X-100 in a molecular form which was excluded from Sepharose 4B. Sepharose 116-128 alanyl aminopeptidase, membrane Sus scrofa 22-36 832424-0 1977 Separation of human renin substrate from renin and a major contaminating albumin using a concanavalin A-sepharose column. Sepharose 104-113 renin Homo sapiens 20-25 832424-1 1977 Human plasma renin substrate was purified and separated from renin by a concanavalin A-Sepharose affinity column. Sepharose 87-96 renin Homo sapiens 13-18 68173-0 1977 Affinity chromatography of mouse alpha-fetoprotein (AFP) on oestradiol-Sepharose adsorbents--isolation and properties. Sepharose 71-80 alpha fetoprotein Mus musculus 33-50 68173-0 1977 Affinity chromatography of mouse alpha-fetoprotein (AFP) on oestradiol-Sepharose adsorbents--isolation and properties. Sepharose 71-80 alpha fetoprotein Mus musculus 52-55 24271849-1 1977 In the present paper we describe the synthesis of Sepharose-boundN-(5"-phosphopyridoxyl)-amino-oxyacetic acid,N-(5"-phosphopyridoxyl)-canaline, andN-(5"-phosphopyridoxyl)-alpha,gamma-diaminobutyric acid (Seph-DAB-PLP), designed for binding specifically brain glutamate decarboxylase (GAD). Sepharose 50-59 proteolipid protein (myelin) 1 Mus musculus 213-216 24271849-1 1977 In the present paper we describe the synthesis of Sepharose-boundN-(5"-phosphopyridoxyl)-amino-oxyacetic acid,N-(5"-phosphopyridoxyl)-canaline, andN-(5"-phosphopyridoxyl)-alpha,gamma-diaminobutyric acid (Seph-DAB-PLP), designed for binding specifically brain glutamate decarboxylase (GAD). Sepharose 50-59 glutamate-ammonia ligase (glutamine synthetase) Mus musculus 259-282 24271849-1 1977 In the present paper we describe the synthesis of Sepharose-boundN-(5"-phosphopyridoxyl)-amino-oxyacetic acid,N-(5"-phosphopyridoxyl)-canaline, andN-(5"-phosphopyridoxyl)-alpha,gamma-diaminobutyric acid (Seph-DAB-PLP), designed for binding specifically brain glutamate decarboxylase (GAD). Sepharose 50-59 glutamate-ammonia ligase (glutamine synthetase) Mus musculus 284-287 616734-6 1977 This inhibitor covalently bound to Sepharose 4B was used for affinity chromatography of cathepsin E. Sepharose 35-47 cathepsin E Oryctolagus cuniculus 88-99 205865-5 1978 Further analysis of the EGF/URO-labeled components by affinity chromatography on concanavalin A-Sepharose, by disc gel electrophoresis, and by enzymatic digestion suggests that the major specific binding component for EGF/URO in liver membranes is a glycoprotein subunit of approximately 100,000 daltons that possesses a 20,000-dalton portion inaccessible to proteolytic cleavage when the subunit is anchored in the membrane. Sepharose 96-105 epidermal growth factor Rattus norvegicus 24-31 911883-1 1977 An affinity chromatograpy method utilising transferrin liganded agarose has been developed for the partial purification of transferrin binding components from Triton X-100 solubilised rabbit reticulocyte plasma membranes. Sepharose 64-71 serotransferrin Oryctolagus cuniculus 43-54 911883-1 1977 An affinity chromatograpy method utilising transferrin liganded agarose has been developed for the partial purification of transferrin binding components from Triton X-100 solubilised rabbit reticulocyte plasma membranes. Sepharose 64-71 serotransferrin Oryctolagus cuniculus 123-134 914865-1 1977 Trypsin inhibitor was isolated from seeds of opaque-2 corn by affinity chromatography on a trypsin/Sepharose column. Sepharose 99-108 Bowman-Birk type bran trypsin inhibitor Zea mays 0-17 411659-0 1977 The preparation of ligandin with glutathione-S-transferase activity from porcine liver cytosol affinity chromatography on bromosulphophthalein-Sepharose. Sepharose 143-152 glutathione S-transferase alpha 2 Rattus norvegicus 19-27 411659-2 1977 After ion-exchange chromatography on DEAE-Sephadex, ligandin is isolated from porcine liver cytosol by affinity chromatography on bromosulphophthalein-Sepharose and gel filtration of Sephadex G-100. Sepharose 151-160 glutathione S-transferase alpha 2 Rattus norvegicus 52-60 607397-3 1977 After passing the membrane protein dissolved in 1% DOC over a Sepharose 4B column, a peak with insulin receptor binding activity has been shown in the void volumn. Sepharose 62-71 insulin receptor Homo sapiens 95-111 71922-0 1977 Preparation of homogeneous human prostatic acid phosphatase using concanavalin A-sepharose 4-B. Sepharose 81-94 acid phosphatase 3 Homo sapiens 33-59 563231-0 1977 Interaction of lipoprotein lipase with heparin-Sepharose. Sepharose 47-56 lipoprotein lipase Bos taurus 15-33 73465-7 1977 This culture system has allowed us to further investigate immunochemical properties of both BALB/c and B10.BR GT-TSF-GT-TSF from both strains display affinity for GT-Sepharose and bear determinants encoded by the I region of the H-2 complex. Sepharose 166-175 granzyme C Mus musculus 103-106 19499-9 1977 Fibrinogen, as assessed by chromatographic experiments with heparin-Sepharose columns, had a considerably lower binding affinity for heparin than did CIg, suggesting that it participates in precipitate formation mainly, if not entirely, by virtue of its affinity for CIg. Sepharose 68-77 fibrinogen beta chain Homo sapiens 0-10 408379-6 1977 When thrombin-activated FVIII/vWF protein was filtered on 4% agarose in 0.15 M NaCl, there was considerable inactivation of FVIII procoagulant activity; however, the procoagulant activity that did remain eluted in the void volume. Sepharose 61-68 coagulation factor II, thrombin Homo sapiens 5-13 408379-6 1977 When thrombin-activated FVIII/vWF protein was filtered on 4% agarose in 0.15 M NaCl, there was considerable inactivation of FVIII procoagulant activity; however, the procoagulant activity that did remain eluted in the void volume. Sepharose 61-68 coagulation factor VIII Homo sapiens 24-29 408379-6 1977 When thrombin-activated FVIII/vWF protein was filtered on 4% agarose in 0.15 M NaCl, there was considerable inactivation of FVIII procoagulant activity; however, the procoagulant activity that did remain eluted in the void volume. Sepharose 61-68 von Willebrand factor Homo sapiens 30-33 408379-6 1977 When thrombin-activated FVIII/vWF protein was filtered on 4% agarose in 0.15 M NaCl, there was considerable inactivation of FVIII procoagulant activity; however, the procoagulant activity that did remain eluted in the void volume. Sepharose 61-68 coagulation factor VIII Homo sapiens 124-129 408379-7 1977 In contrast, when thrombin-activated FVIII/vWF protein was filtered in 0.25 M CaCl(2), the FVIII procoagulant activity eluted well after the void volume and remained activated for 6 h. The procoagulant peak isolated by filtering nonthrombin-activated FVIII/vWF protein on agarose in 0.25 M CaCl(2) was compared to that isolated from thrombin-activated FVIII/vWF protein. Sepharose 272-279 coagulation factor II, thrombin Homo sapiens 18-26 408379-7 1977 In contrast, when thrombin-activated FVIII/vWF protein was filtered in 0.25 M CaCl(2), the FVIII procoagulant activity eluted well after the void volume and remained activated for 6 h. The procoagulant peak isolated by filtering nonthrombin-activated FVIII/vWF protein on agarose in 0.25 M CaCl(2) was compared to that isolated from thrombin-activated FVIII/vWF protein. Sepharose 272-279 coagulation factor VIII Homo sapiens 37-42 408379-7 1977 In contrast, when thrombin-activated FVIII/vWF protein was filtered in 0.25 M CaCl(2), the FVIII procoagulant activity eluted well after the void volume and remained activated for 6 h. The procoagulant peak isolated by filtering nonthrombin-activated FVIII/vWF protein on agarose in 0.25 M CaCl(2) was compared to that isolated from thrombin-activated FVIII/vWF protein. Sepharose 272-279 von Willebrand factor Homo sapiens 43-46 408379-7 1977 In contrast, when thrombin-activated FVIII/vWF protein was filtered in 0.25 M CaCl(2), the FVIII procoagulant activity eluted well after the void volume and remained activated for 6 h. The procoagulant peak isolated by filtering nonthrombin-activated FVIII/vWF protein on agarose in 0.25 M CaCl(2) was compared to that isolated from thrombin-activated FVIII/vWF protein. Sepharose 272-279 coagulation factor VIII Homo sapiens 91-96 408379-7 1977 In contrast, when thrombin-activated FVIII/vWF protein was filtered in 0.25 M CaCl(2), the FVIII procoagulant activity eluted well after the void volume and remained activated for 6 h. The procoagulant peak isolated by filtering nonthrombin-activated FVIII/vWF protein on agarose in 0.25 M CaCl(2) was compared to that isolated from thrombin-activated FVIII/vWF protein. Sepharose 272-279 coagulation factor VIII Homo sapiens 91-96 408379-7 1977 In contrast, when thrombin-activated FVIII/vWF protein was filtered in 0.25 M CaCl(2), the FVIII procoagulant activity eluted well after the void volume and remained activated for 6 h. The procoagulant peak isolated by filtering nonthrombin-activated FVIII/vWF protein on agarose in 0.25 M CaCl(2) was compared to that isolated from thrombin-activated FVIII/vWF protein. Sepharose 272-279 coagulation factor VIII Homo sapiens 91-96 408379-11 1977 Taken together, these results imply that thrombin generates the FVIII procoagulant activity that is stabilized by 0.25 M CaCl(2) and elutes aberrantly from 4% agarose in that solvent. Sepharose 159-166 coagulation factor II, thrombin Homo sapiens 41-49 408379-11 1977 Taken together, these results imply that thrombin generates the FVIII procoagulant activity that is stabilized by 0.25 M CaCl(2) and elutes aberrantly from 4% agarose in that solvent. Sepharose 159-166 coagulation factor VIII Homo sapiens 64-69 198005-1 1977 The glycoprotein nature of renin isolated from either rabbit or human kidney has been demonstrated by affinity chromatography on concanavalin A-Sepharose. Sepharose 144-153 LOW QUALITY PROTEIN: renin Oryctolagus cuniculus 27-32 408346-2 1977 A lectin, whose specific activity in soluble extracts of embryonic chick pectoral muscle increases strikingly between 8 and 16 days of development, has been purified by affinity chromatography on derivatized Sepharose 4B coupled to p-aminophenyl-beta-D-lactoside. Sepharose 208-220 galectin 3 Gallus gallus 2-8 198071-1 1977 A method of affinity chromatography based on the trapping of actin filaments within agarose gel beads is described. Sepharose 84-91 actin Oryctolagus cuniculus 61-66 891468-2 1977 The antiserum gave precipitin lines of complete identity to phosvitin and to vitellogenin which was isolated from hen plasma by DEAE-cellulose chromatography and by affinity chromatography using anti-phosvitin coupled to Sepharose 4B. Sepharose 221-233 putative uncharacterized protein LOC400499 Homo sapiens 77-89 199582-4 1977 On the other hand, all of these phosphatidylcholines acted as substrates of LCAT when they were incorporated into HDL coupled to Sepharose. Sepharose 129-138 lecithin-cholesterol acyltransferase Homo sapiens 76-80 893640-0 1977 Purification of alanine aminotransferase from human serum on a cycloserinederivatized agarose. Sepharose 86-93 glutamic--pyruvic transaminase Homo sapiens 16-40 915013-1 1977 In a study of the lipoprotein pattern in multiple myelomatosis electrophoresis on agarose gel showed abnormal lipoproteins, named paralipoproteins (p-Lp), in 24 out of 30 normolipidaemic patients. Sepharose 82-89 peroxiredoxin 5 Homo sapiens 130-152 337461-7 1977 Oligoclonal IgG was found in CSF by agarose gel electrophoresis in 88% of the patients. Sepharose 36-43 colony stimulating factor 2 Homo sapiens 29-32 409660-1 1977 The phenotypes of glyoxalase I (GLO) were determined in a random population from Hessen (Germany) by high-voltage agarose gel electrophoresis. Sepharose 115-122 glyoxalase I Homo sapiens 32-35 579491-2 1977 In the first step of the preparation, using heparin-agarose chromatography, we observed that the complexed form of AT III bound less strongly to the gel than the free form and that about half of the AT III was free. Sepharose 52-59 serpin family C member 1 Homo sapiens 115-121 69627-6 1977 The sedimentation rate of the reverse transcriptase - p30 complex is approximately 12 S as estimated by glycerol gradient centrifugation, and the molecular weight is approximately 400,000 by chromatography on a Sepharose 6B column. Sepharose 211-220 centromere protein V Homo sapiens 54-57 70227-8 1977 The slow moving alpha-fetoprotein could be further fractionated on RCAI-sepharose column in two components, AFPA1 and AFPA2 differing by their sialic acid content. Sepharose 72-81 alpha-fetoprotein Rattus norvegicus 16-33 560871-1 1977 A bovine counterpart to human prealbumin was purified from bovine serum by thiol-disulfide exchange chromatography on thiol-Sepharose 4B and affinity chromatography on human retinol-binding protein linked to Sepharose 4B. Sepharose 124-133 transthyretin Bos taurus 30-40 560871-1 1977 A bovine counterpart to human prealbumin was purified from bovine serum by thiol-disulfide exchange chromatography on thiol-Sepharose 4B and affinity chromatography on human retinol-binding protein linked to Sepharose 4B. Sepharose 208-217 transthyretin Bos taurus 30-40 560871-2 1977 The bovine prealbumin had alpha1-mobility on agarose gel electrophoresis at pH 8.6. Sepharose 45-52 transthyretin Bos taurus 11-21 560871-7 1977 This property was used to isolate a protein from bovine serum, by chromatography on bovine prealbumin linked to Sepharose which cross-reacted with antiserum against human retinol-binding protein; had a molecular weight of 21 000 and alpha 2-mobility on agarose gel electrophoresis. Sepharose 112-121 transthyretin Bos taurus 91-101 877583-1 1977 Syn-and anti-benzo[a]pyrene diol epoxides elicit a concentration-dependent nicking of superhelical Col E1 DNA in an in vitro reaction monitored by agarose gel electrophoresis and electron microscopy. Sepharose 147-154 synemin Homo sapiens 0-3 872091-2 1977 The CEA-like activity in these lavages was purified by gel filtration on Sepharose 6B and Sephadex G-200 and by affinity chromatography on concanavalin A linked to Sepharose. Sepharose 73-82 CEA cell adhesion molecule 3 Homo sapiens 4-7 69448-1 1977 Ovine alpha-fetoprotein was successfully isolated from fetal sheep serum by using rabbit anti-ovine alpha-fetoprotein linked to an agarose immunoadsorbent column. Sepharose 131-138 alpha fetoprotein Homo sapiens 6-23 353657-6 1977 When heparin is incorporated in the agarose plate, the electrophoretic mobility of this polymerized antithrombin III is not modified. Sepharose 36-43 serpin family C member 1 Homo sapiens 100-116 617780-1 1977 Affinity chromatographic studies using insolubilized fibrinogen (fibrinogen-agarose) revealed that fibrin monomer present in plasma is selectively adsorbed to fibrinogen-agarose and may be quantitatively estimated following desorption. Sepharose 76-83 fibrinogen beta chain Homo sapiens 53-63 596302-1 1977 Cathepsin D was purified from human liver by a procedure involving autolysis, acetone fractionation, and chromatography on ion-exchange media and organomercurial-sepharose. Sepharose 162-171 cathepsin D Homo sapiens 0-11 617780-1 1977 Affinity chromatographic studies using insolubilized fibrinogen (fibrinogen-agarose) revealed that fibrin monomer present in plasma is selectively adsorbed to fibrinogen-agarose and may be quantitatively estimated following desorption. Sepharose 76-83 fibrinogen beta chain Homo sapiens 65-75 617780-1 1977 Affinity chromatographic studies using insolubilized fibrinogen (fibrinogen-agarose) revealed that fibrin monomer present in plasma is selectively adsorbed to fibrinogen-agarose and may be quantitatively estimated following desorption. Sepharose 76-83 fibrinogen beta chain Homo sapiens 65-75 617780-1 1977 Affinity chromatographic studies using insolubilized fibrinogen (fibrinogen-agarose) revealed that fibrin monomer present in plasma is selectively adsorbed to fibrinogen-agarose and may be quantitatively estimated following desorption. Sepharose 170-177 fibrinogen beta chain Homo sapiens 65-75 617780-1 1977 Affinity chromatographic studies using insolubilized fibrinogen (fibrinogen-agarose) revealed that fibrin monomer present in plasma is selectively adsorbed to fibrinogen-agarose and may be quantitatively estimated following desorption. Sepharose 170-177 fibrinogen beta chain Homo sapiens 65-75 192341-0 1977 [Cytochrome c immobilized on Sepharose 4B and its participation in photochemical reactions of chloroplasts]. Sepharose 29-38 cytochrome c, somatic Homo sapiens 1-13 192341-1 1977 Cytochrome c immobilized on cyanogen bromide-activated Sepharose 4B may be used to study photochemical reactions in chloroplasts. Sepharose 55-67 cytochrome c, somatic Homo sapiens 0-12 558000-1 1977 The interaction between porcine pancreatic phospholipase A2 and low-molecular fragments of its substrate -- lecithine was studied using gel-diffusion of the enzyme in lecithin-agarose plates. Sepharose 176-183 phospholipase A2 group IB Homo sapiens 43-59 892174-0 1977 The stability of lipoamide dehydrogenase immobilized to agarose through spacer molecules of various lengths. Sepharose 56-63 dihydrolipoamide dehydrogenase Homo sapiens 17-40 139915-1 1977 Myosin was isolated from cultured human endothelial cells by extraction with 0.6 M KCl and chromatography on Sepharose 4B. Sepharose 109-121 myosin heavy chain 14 Homo sapiens 0-6 344037-2 1977 The enzyme-inhibiting fraction was coupled to Sepharose and the resin obtained was used for, affinity-chromatography purification of the GnRH-degrading enzyme. Sepharose 46-55 gonadotropin releasing hormone 1 Rattus norvegicus 137-141 344037-5 1977 Bacitracin, an effective peptide inhibitor of the degradation of GnRH, was also coupled to Sepharose. Sepharose 91-100 gonadotropin releasing hormone 1 Rattus norvegicus 65-69 344037-6 1977 Three different such Sepharose-bacitracin conjugates were synthesized, two of which inhibited the degradation of GnRH by hypothalamic and pituitary extracts. Sepharose 21-30 gonadotropin releasing hormone 1 Rattus norvegicus 113-117 870397-2 1977 Platelets from a FXI-deficient patient treated in a similar fashion also released a coagulant activity which could be absorbed onto Sepharose-heparin and eluted similarly to plasma FXI. Sepharose 132-141 coagulation factor XI Homo sapiens 17-20 402327-2 1977 Antithrombin III (AT-III), being an alpha2-globulin, will have an electrophoretic mobility in the presence of heparin like prealbumin in agarose gels. Sepharose 137-144 serpin family C member 1 Homo sapiens 0-16 407034-2 1977 The method is based on batch-wise treatment of serum with hydroxyapatite followed by batch-wise adsorption of TBG to an affinity gel consisting of Sepharose to which thyroxine was bound with a spacer group. Sepharose 147-156 serpin family A member 7 Homo sapiens 110-113 408067-5 1977 Evidence is presented that immunodiffusion values of properdin might be affected by precipitation of a C3-properdin complex in gels containing Mg2+ ions after the activation of the properdin system by agarose. Sepharose 201-208 complement factor properdin Homo sapiens 53-62 576231-4 1977 TgA was prepared by dissolving the immune precipitate (formed with human thyroglobulin [Tg] and human anti-Tg) in excess Tg and chromatographing the mixture on Sepharose 4B. Sepharose 160-169 T-box transcription factor 1 Homo sapiens 0-3 402327-2 1977 Antithrombin III (AT-III), being an alpha2-globulin, will have an electrophoretic mobility in the presence of heparin like prealbumin in agarose gels. Sepharose 137-144 serpin family C member 1 Homo sapiens 18-24 402327-3 1977 This phenomenon was utilized to quantitate AT-III from serum and plasma by electroimmunodiffusion (EID) for 90 min agarose gels containing 75 USP units of heparin/ml gel. Sepharose 115-122 serpin family C member 1 Homo sapiens 43-49 267293-5 1977 During the acute phase of the disease the Factor VIII related protein was tested for its ristocetin cofactor activity, its electrophoretic mobility on crossed antigen-antibody electrophoresis and its elution pattern on Sepharose 4B columns; all these properties proved to be abnormal. Sepharose 219-228 cytochrome c oxidase subunit 8A Homo sapiens 49-53 557070-1 1977 Insulin was directly coupled and also indirectly coupled by a side chain to Sepharose 4B to form immunoabsorbents for affinity chromatography of insulin antibodies. Sepharose 76-88 insulin Homo sapiens 0-7 557070-1 1977 Insulin was directly coupled and also indirectly coupled by a side chain to Sepharose 4B to form immunoabsorbents for affinity chromatography of insulin antibodies. Sepharose 76-88 insulin Homo sapiens 145-152 27644-1 1977 Calf brain prolidase covalently bound to CNBr-Sepharose 4B, retained about 32% of the activity of the uncoupled enzyme. Sepharose 46-55 peptidase D Bos taurus 11-20 141952-5 1977 Solubilization of the 5"-nucleotidase does not prevent the effect of Con A and the solubilized enzyme is firmly bound by Con A-Sepharose 4B; these results suggest that Con A inhibits the enzyme by a direct interaction and that 5"-nucleotidase can be considered as an eventual receptor for the lectin. Sepharose 127-136 CONA Sus scrofa 121-126 141952-5 1977 Solubilization of the 5"-nucleotidase does not prevent the effect of Con A and the solubilized enzyme is firmly bound by Con A-Sepharose 4B; these results suggest that Con A inhibits the enzyme by a direct interaction and that 5"-nucleotidase can be considered as an eventual receptor for the lectin. Sepharose 127-136 CONA Sus scrofa 121-126 333629-0 1977 [Advantages and technical conditions of the migration inhibition test [MIF] in agarose gel (author"s transl)]. Sepharose 79-86 macrophage migration inhibitory factor Homo sapiens 71-74 857425-1 1977 A human antibody which preferentially agglutinates p erythrocytes is inhibited specifically by the glycolipid sialosylparagloboside, NeuNAc (alpha,2 leads to 3)Gal(beta,1 leads t0 4)GlcNAc(beta,1 leads to 3)Gal(beta,1 leads to 4)Glc-Cer and forms a precipitin band with this compound in agarose gel. Sepharose 287-294 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 164-170 857425-1 1977 A human antibody which preferentially agglutinates p erythrocytes is inhibited specifically by the glycolipid sialosylparagloboside, NeuNAc (alpha,2 leads to 3)Gal(beta,1 leads t0 4)GlcNAc(beta,1 leads to 3)Gal(beta,1 leads to 4)Glc-Cer and forms a precipitin band with this compound in agarose gel. Sepharose 287-294 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 189-195 857425-1 1977 A human antibody which preferentially agglutinates p erythrocytes is inhibited specifically by the glycolipid sialosylparagloboside, NeuNAc (alpha,2 leads to 3)Gal(beta,1 leads t0 4)GlcNAc(beta,1 leads to 3)Gal(beta,1 leads to 4)Glc-Cer and forms a precipitin band with this compound in agarose gel. Sepharose 287-294 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 189-195 595780-0 1977 [Preparation of esterase D phenotypes of erythrocytes using 4-methylumbelliferone heptanoate after electrophoresis in agar-agarose gel]. Sepharose 123-130 esterase D Homo sapiens 16-26 826537-2 1976 Lipoprotein lipase of high purity has been isolated from bovine milk by affinity chromatography on heparin-Sepharose, adsorption to Cgamma-aluminum hydroxide gel, and intervent dilution chromatography on heparin-Sepharose. Sepharose 107-116 lipoprotein lipase Bos taurus 0-18 826537-2 1976 Lipoprotein lipase of high purity has been isolated from bovine milk by affinity chromatography on heparin-Sepharose, adsorption to Cgamma-aluminum hydroxide gel, and intervent dilution chromatography on heparin-Sepharose. Sepharose 212-221 lipoprotein lipase Bos taurus 0-18 1009091-1 1976 Lipoprotein lipase has been purified from bovine milk by affinity chromatography on Sepharose containing covalently linked heparin. Sepharose 84-93 lipoprotein lipase Bos taurus 0-18 1087162-4 1976 Two additional products are identical to fragments of alpha-1-antitrypsin which can be washed from a column of Sepharose-bound elastase immediately after alpha-1-antitrypsin is applied to the column. Sepharose 111-120 serpin family A member 1 Homo sapiens 54-73 846962-1 1977 UDP-glucose dehydrogenase was purified from an extract of calf liver acetone powder by sequential chromatography on norleucine-agarose, Type 2, agarose-hexane-AMP, and UDP-hexanolamine-Sepharose. Sepharose 127-134 UDP-glucose 6-dehydrogenase Bos taurus 0-25 846962-1 1977 UDP-glucose dehydrogenase was purified from an extract of calf liver acetone powder by sequential chromatography on norleucine-agarose, Type 2, agarose-hexane-AMP, and UDP-hexanolamine-Sepharose. Sepharose 185-194 UDP-glucose 6-dehydrogenase Bos taurus 0-25 1002695-1 1976 Rat liver albumin messenger RNA has been purified to apparent homogeneity by means of polysome immunoprecipitation and poly(U)-Sepharose affinity chromatography. Sepharose 127-136 albumin Rattus norvegicus 10-17 65984-0 1976 Interactions of cytochrome c peroxidase and of its apoprotein with sepharose-bound cytochrome c [proceedings]. Sepharose 67-76 cytochrome c, somatic Homo sapiens 16-28 65984-0 1976 Interactions of cytochrome c peroxidase and of its apoprotein with sepharose-bound cytochrome c [proceedings]. Sepharose 67-76 cytochrome c, somatic Homo sapiens 83-95 1008824-5 1976 With Sepharose-myosin columns (myosin covalently coupled to CNBr-activated Sepharose) a dissociation constant of 1.8 muM for ATP4- was found. Sepharose 5-14 myosin heavy chain 14 Homo sapiens 15-21 63339-3 1976 The complexes between cationic protein and alpha1-antichymotrypsin migrate as beta-globulin on agarose gel electrophoresis. Sepharose 95-102 serpin family A member 3 Homo sapiens 43-66 24420666-4 1977 Heterologous ferredoxin (i.e., ferredoxin other than from N. tabacum) was coupled covalently to Sepharose beads. Sepharose 96-105 ferredoxin Nicotiana tabacum 13-23 24420666-4 1977 Heterologous ferredoxin (i.e., ferredoxin other than from N. tabacum) was coupled covalently to Sepharose beads. Sepharose 96-105 ferredoxin Nicotiana tabacum 31-41 827443-2 1976 Human coagulation factor IX was purified by two ion-exchange chromatographies on DEAE-Sephadex A-50, heparin-Sepharose chromatography, hydroxyapatite chromatography and immunoadsorbent technique. Sepharose 109-118 coagulation factor IX Homo sapiens 6-27 15110-4 1976 The degrading effect on articular cartilage proteoglycans (PGC and PGS), isolated from the same joints, was demonstrated by gelfiltration on Sepharose columns and by viscometry. Sepharose 141-150 progastricsin Bos taurus 59-62 993204-3 1976 Throughout the purification procedures which involved adsorption onto sulfopropyl (SP)-Sephadex, chromatography on Sephadex G-25 and SP-Sephadex, immunoadsorption on neurotensin-antibody Sepharose and high voltage paper electrophoresis, R-NT displayed the chromatographic and electrophoretic properties of neurotensin. Sepharose 187-196 neurotensin Bos taurus 166-177 1000849-0 1976 Effect of agarose variability on the measurement of lysozyme activity. Sepharose 10-17 lysozyme Homo sapiens 52-60 1000849-1 1976 Lysozyme assays are often performed by a diffusion technique utilizing agarose gels impregnated with substrate organisms (lysoplates), but the results differ greatly from those obtained with spectrophotometric or immunologic techniques. Sepharose 71-78 lysozyme Homo sapiens 0-8 1000849-4 1976 The different agaroses had variable effects on determinations of normal serum lysozyme, and the results obtained on any given gel agreed with neither those found on other gels nor with independent assay in another system. Sepharose 14-22 lysozyme Homo sapiens 78-86 1071595-4 1976 Des-angiotensin substrate was prepared from the purified angiotensinogen preparation by reaction with immobilized hog renin (coupled to Sepharose). Sepharose 136-145 renin Rattus norvegicus 118-123 992564-4 1976 The most rapid and convenient method, a modification of the affinity chromatography on GMP agarose first described by Hughes[5] gives hypoxanthine phosphoribosyltransferase which is superior to the other preparations in its homogeneity and its specific activity. Sepharose 91-98 hypoxanthine phosphoribosyltransferase 1 Rattus norvegicus 134-172 990260-3 1976 Galactosyltransferase (UDPgalactose:glycoprotein galactosyltransferase EC 2.4.1.22) was isolated from swine mesentary lymhromatography on Sepharose 4B colums containing covalently bound p-aminophenyl-beta-D-N-acetylglucosamine. Sepharose 138-150 N-acetyllactosaminide alpha-1,3-galactosyltransferase Sus scrofa 0-21 991975-0 1976 Synthesis of S-lactoyl-glutathione using glyoxalase I bound to sepharose 4B. Sepharose 63-72 glyoxalase I Homo sapiens 41-53 991975-1 1976 Glyoxalase I bound to Sepharose 4B was used for synthesis of S-lactoyl-glutathione. Sepharose 22-31 glyoxalase I Homo sapiens 0-12 1000364-0 1976 The purification of porcine haptoglobin by affinity chromatography with concanavalin A-Sepharose. Sepharose 87-96 haptoglobin Homo sapiens 28-39 977599-7 1976 In 6 M guanidine HCl, hydroxyapatite-purified alpha-actinin has a molecular weight of 106,000 +/- 6,300 as determined by sedimentation equilibrium and a molecular weight of 100,000 as determined by a calibrated 4% agarose gel permeation column. Sepharose 214-221 actinin alpha 1 Homo sapiens 46-59 977599-10 1976 By assuming 45% hydration and a molecular weight of 206,000, dimensions of approximately 40 X 500 A can be calculated for the alpha-actinin molecule by using either s 020, w, D 020, w, intrinsic viscosity, or a calibrated 6% agarose gel permeation column. Sepharose 225-232 actinin alpha 1 Homo sapiens 126-139 1008906-4 1976 With heparin-Sepharose 4 B affinity chromatography it was possible to partially purify human adipose tissue lipoprotein lipase (LPL) as well as a lipase from human liver. Sepharose 13-22 lipoprotein lipase Homo sapiens 108-126 1008906-4 1976 With heparin-Sepharose 4 B affinity chromatography it was possible to partially purify human adipose tissue lipoprotein lipase (LPL) as well as a lipase from human liver. Sepharose 13-22 lipoprotein lipase Homo sapiens 128-131 1035501-2 1976 The complex of rat muscle glyceraldehyde-3-phosphate dehydrogenase (GAPD), immobilized on Sepharose 4B, with anti-rat GAPD rabbit antibodies was digested with papain. Sepharose 90-102 glyceraldehyde-3-phosphate dehydrogenase Rattus norvegicus 26-66 1035501-2 1976 The complex of rat muscle glyceraldehyde-3-phosphate dehydrogenase (GAPD), immobilized on Sepharose 4B, with anti-rat GAPD rabbit antibodies was digested with papain. Sepharose 90-102 glyceraldehyde-3-phosphate dehydrogenase Rattus norvegicus 68-72 188648-0 1976 Purification of poly(ADP-ribose) polymerase from Ehrlich ascites tumor cells by chromatography on DNA-agarose. Sepharose 102-109 poly (ADP-ribose) polymerase family, member 1 Mus musculus 16-43 188648-1 1976 Poly(ADP-ribose) polymerase with a high specific activity was obtained from Ehrlich ascites tumor cells by extraction of nuclei with 175 mM potassium phosphate, followed by chromatography on DNA-agarose. Sepharose 195-202 poly (ADP-ribose) polymerase family, member 1 Mus musculus 0-27 827597-2 1976 However, a thyroglobulin-immunoreactive iodoprotein was isolated by affinity chromatography on agarose gel to which antibody against thyroglobulin had been covalently bound. Sepharose 95-102 thyroglobulin Ovis aries 11-24 977565-4 1976 An enriched antibody preparation bound to Sepharose was used as an immunosorbent to purify gp70 over 600-fold in one step with a 30% recovery. Sepharose 42-51 embigin Mus musculus 91-95 186782-4 1976 Sodium periodate oxidation of lysosomal beta-glucuronidase resulted in a near abolition of rapid clearance, a reduction in concanavilin-A-Sepharose binding, and a reduction in neutral sugar content, accompanied by alteration in isoelectric focusing properties. Sepharose 138-147 glucuronidase, beta Rattus norvegicus 40-58 977572-3 1976 The solubilized nAChR was partially purified by affinity chromatography (Naja naja siamensis alpha-toxin affinity arm, linked to Sepharose 4B) and characterized by binding of 125I-labeled alpha-bungarotoxin. Sepharose 129-138 cholinergic receptor nicotinic beta 1 subunit Rattus norvegicus 16-21 184090-1 1976 Cytochrome P-450 was purified from bovine adrenal cortex mitochondria by affinity chromatography using an octylamine-substituted Sepharose column. Sepharose 129-138 cytochrome P450 family 4 subfamily F member 3 Homo sapiens 0-16 972146-3 1976 These proteins remain associated with the poly(A)-rich RNA during affinity chromatography of RNase A and T1-digested polyribosomes on poly(U)-Sepharose in 0.5 M NaCl, and co-elute from the column with the RNA at 50% formamide. Sepharose 142-151 ribonuclease A family member 1, pancreatic Homo sapiens 93-100 1084744-3 1976 "Spontaneous" anti-EB2 cytotoxicity was not reduced by preincubation of the effector cells with plastic or iron carbonyl or by passage through cotton wool or agarose columns but was reduced by passage through nylon wool columns. Sepharose 158-165 microtubule associated protein RP/EB family member 2 Homo sapiens 19-22 953987-4 1976 Optimal MAF levels followed incubation of lymphocytes for 48 hr with Sepharose-bound concanavalin A. Sepharose 69-78 MAF bZIP transcription factor Rattus norvegicus 8-11 974043-3 1976 When highly purified human factor VIII is submitted to agarose gel chromatography in the presence of 0.5 M CaCl2, the procoagulant activity (low molecular weight factor VIII, LMW-F VIII) is separated from the void volume protein (Vo-VIII). Sepharose 55-62 cytochrome c oxidase subunit 8A Homo sapiens 34-38 974043-3 1976 When highly purified human factor VIII is submitted to agarose gel chromatography in the presence of 0.5 M CaCl2, the procoagulant activity (low molecular weight factor VIII, LMW-F VIII) is separated from the void volume protein (Vo-VIII). Sepharose 55-62 cytochrome c oxidase subunit 8A Homo sapiens 169-173 974043-3 1976 When highly purified human factor VIII is submitted to agarose gel chromatography in the presence of 0.5 M CaCl2, the procoagulant activity (low molecular weight factor VIII, LMW-F VIII) is separated from the void volume protein (Vo-VIII). Sepharose 55-62 cytochrome c oxidase subunit 8A Homo sapiens 169-173 974043-3 1976 When highly purified human factor VIII is submitted to agarose gel chromatography in the presence of 0.5 M CaCl2, the procoagulant activity (low molecular weight factor VIII, LMW-F VIII) is separated from the void volume protein (Vo-VIII). Sepharose 55-62 cytochrome c oxidase subunit 8A Homo sapiens 169-173 793749-1 1976 Using an assay of macrophage migration, where the cells emigrate from an agarose droplet, it was found that the neutral proteases trypsin, chymotrypsin, Pronase and elastase have MIF-like activity. Sepharose 73-80 macrophage migration inhibitory factor Homo sapiens 179-182 993733-0 1976 Isolation of rat transferrin using CNBr-activated sepharose 4B. Sepharose 50-59 transferrin Rattus norvegicus 17-28 996366-5 1976 The fastest (2-day) and simplest procedure for a routine 10-fold purification of kininogen from plasma consisted of ammonium sulfate precipitation (33-46%) followed by a single passage through a concanavalin A-agarose column. Sepharose 210-217 kininogen 2-like 1 Rattus norvegicus 81-90 993733-2 1976 The isolation of transferrin from rat serum by means of affinity chromatography on CNBr-activated Sepharose 4 B is described. Sepharose 98-107 transferrin Rattus norvegicus 17-28 134997-1 1976 A functionally active human plasmin light (B) chain derivative, stabilized by the streptomyces plasmin inhibitor leupeptin, was isolated from a partially reduced and alkylated enzyme preparation by an affinity chromatography method with a L-lysine-substituted Sepharose column. Sepharose 260-269 plasminogen Homo sapiens 28-35 956190-1 1976 Human fibroblast interferon binds to L-tryptophan, D-tryptophan, L-phenylalanine, and L-tyrosine, all immobilized directly to cyanogen bromide-activated agarose, as well as to L-tryptophan and D-tryptophan methyl ester, both immobilized via molecular arms. Sepharose 153-160 interferon beta 1 Homo sapiens 6-27 61075-1 1976 The electroimmunodiffusion on agarose has been applied for the determination of AFP in human serum. Sepharose 30-37 alpha fetoprotein Homo sapiens 80-83 184224-9 1976 The complete inhibition of triglyceride removal by an antiserum prepared against adipose tissue LPL demonstrates that the NaCl-inhibited, serum-activated lipase prepared by affinity chromatography on heparin-Sepharose and concanavalin A-Sepharose columns is the enzyme responsible in vivo for the catabolism of VLDL triglyceride. Sepharose 208-217 lipoprotein lipase Oryctolagus cuniculus 96-99 824284-1 1976 L-Homoarginine benzylester was coupled with Sepharose 4B and used for affinity chromatography of human plasma kallikrein [EC 3.4.21.8] and urokinase [EC 3.4.99.26]. Sepharose 44-53 kallikrein related peptidase 4 Homo sapiens 110-120 184224-9 1976 The complete inhibition of triglyceride removal by an antiserum prepared against adipose tissue LPL demonstrates that the NaCl-inhibited, serum-activated lipase prepared by affinity chromatography on heparin-Sepharose and concanavalin A-Sepharose columns is the enzyme responsible in vivo for the catabolism of VLDL triglyceride. Sepharose 237-246 lipoprotein lipase Oryctolagus cuniculus 96-99 1036803-2 1976 Eight groups of fibrinogen derivatives could be separated by gel filtration through 6% agarose in large columns, four with an elution volume smaller and four groups with an elution volume larger than that of fibrinogen. Sepharose 87-94 fibrinogen beta chain Homo sapiens 16-26 186779-1 1976 A three-step procedure including affinity chromatography on NAD+-azobenzamidopropyl-Sepharose has been designed for the purification of yeast glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12] with maximized specific activity and maximized homogeneity with respect to affinity for the coenzyme, NAD+. Sepharose 83-93 oxidoreductase Saccharomyces cerevisiae S288C 219-233 182242-2 1976 Apolipoprotein E ("arginine-rich" polypeptide) was isolated from delipidized human very low density lipoproteins by agarose column chromatography in the presence of 6 M guanidine-hydrochloride. Sepharose 116-123 apolipoprotein E Homo sapiens 0-16 1085169-1 1976 alpha 1-Antitrypsin phenotypes Pi M and Z, purified by the thiol-disulfide exchange procedure, were desialylated by treatment with neuraminidase covalently coupled to Sepharose and used as acceptors of sialic acid in an assay system for serum sialic acid transferase (CMP-N-acetylneuraminate:D-galactosyl-glycoprotein N-acetylneuraminyltransferase, EC 2.4.99.1) activity. Sepharose 167-176 serpin family A member 1 Homo sapiens 0-19 8169-5 1976 It was shown that acriflavin-Sepharose preparations function as NAD(P)H oxidizing agents in a number of different dehydrogenase systems including lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), alanine dehydrogenase (alaDH), and glutamate dehydrogenase (GDH). Sepharose 29-38 malic enzyme 1 Homo sapiens 204-224 8169-5 1976 It was shown that acriflavin-Sepharose preparations function as NAD(P)H oxidizing agents in a number of different dehydrogenase systems including lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), alanine dehydrogenase (alaDH), and glutamate dehydrogenase (GDH). Sepharose 29-38 malic enzyme 1 Homo sapiens 226-229 8169-5 1976 It was shown that acriflavin-Sepharose preparations function as NAD(P)H oxidizing agents in a number of different dehydrogenase systems including lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), alanine dehydrogenase (alaDH), and glutamate dehydrogenase (GDH). Sepharose 29-38 glutamate dehydrogenase 1 Homo sapiens 267-290 8169-5 1976 It was shown that acriflavin-Sepharose preparations function as NAD(P)H oxidizing agents in a number of different dehydrogenase systems including lactate dehydrogenase (LDH), alcohol dehydrogenase (ADH), malate dehydrogenase (MDH), alanine dehydrogenase (alaDH), and glutamate dehydrogenase (GDH). Sepharose 29-38 glutamate dehydrogenase 1 Homo sapiens 292-295 9068-2 1976 Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate-NADP+ oxidoreductase, EC 1.1.1.49) from baker"s yeast (Saccharomyces cerevisiae) was immobilized on CNBr-activated Sepharose 4B with retention of about 3% of enzyme activity. Sepharose 172-184 glucose-6-phosphate dehydrogenase Saccharomyces cerevisiae S288C 0-33 9068-13 1976 Glucose 6-phosphate dehydrogenase immobilized on CNBr-activated Sephadex G-25 was unstable, as was enzyme attached to CNBr-activated Sepharose 4B to which glycine, asparitic acid, valine or ethylenediamine was added at the same time as the enzyme. Sepharose 133-145 glucose-6-phosphate dehydrogenase Saccharomyces cerevisiae S288C 0-33 59642-2 1976 We present a method that does not have these serious disadvantages, and with which alpha1-fetoprotein was purified by sequential use of concanvalin A affinity-chromatography, preparative gel-electrophoresis, and immunoabsorption with anti-albumin antibody covalently coupled to Sepharose 4B. Sepharose 278-287 alpha fetoprotein Homo sapiens 83-101 821601-3 1976 The mucin was purified by Sepharose 4B and 2B column chromatography of high-speed supernatant fractions. Sepharose 26-35 LOC100508689 Homo sapiens 4-9 8108-1 1976 Beta-Glucuronidase (EC 3.2.1.31) has been isolated from rat-liver microsomes by a novel chromatographic method employing antibody to rat preputial gland beta-glucuronidase coupled to Sepharose. Sepharose 183-192 glucuronidase, beta Rattus norvegicus 0-18 8061-1 1976 Human heart lipoprotein lipase was purified by affinity chromatography on heparin-Sepharose 4B. Sepharose 82-94 lipoprotein lipase Homo sapiens 12-30 58713-3 1976 The affinity chromatography of CEA-M on an immunosorbent "anti-H-Sepharose" demonstrated that a proportion of CEA-M molecules might bear both H and CEA antigenic determinants. Sepharose 65-74 CEA cell adhesion molecule 3 Homo sapiens 31-34 58713-3 1976 The affinity chromatography of CEA-M on an immunosorbent "anti-H-Sepharose" demonstrated that a proportion of CEA-M molecules might bear both H and CEA antigenic determinants. Sepharose 65-74 CEA cell adhesion molecule 3 Homo sapiens 110-113 58713-3 1976 The affinity chromatography of CEA-M on an immunosorbent "anti-H-Sepharose" demonstrated that a proportion of CEA-M molecules might bear both H and CEA antigenic determinants. Sepharose 65-74 CEA cell adhesion molecule 3 Homo sapiens 110-113 829158-1 1976 A substrate of diamine oxidase hexamethylene diamine was covalently bound through adipinic acid dihydrazide to Sepharose 4B in order to prepare a sorbent for the purification of diamine oxidase by means of biospecific (affinity) chromatography. Sepharose 111-123 amine oxidase copper containing 1 Sus scrofa 15-30 829158-1 1976 A substrate of diamine oxidase hexamethylene diamine was covalently bound through adipinic acid dihydrazide to Sepharose 4B in order to prepare a sorbent for the purification of diamine oxidase by means of biospecific (affinity) chromatography. Sepharose 111-123 amine oxidase copper containing 1 Sus scrofa 178-193 1026941-1 1976 Pancreatic Kallikrein was purified by affinity chromatography on BPTI-Sepharose. Sepharose 70-79 kallikrein related peptidase 4 Homo sapiens 11-21 1026941-4 1976 Trypsin occurring in commercial Kallikrein preparations was separated by the batch procedure with ovomucoid-Sepharose. Sepharose 108-117 kallikrein related peptidase 4 Homo sapiens 32-42 932002-9 1976 While the binding of ovalbumin to galactosyltransferase in the presence of Mn2+ alone can be observed by ultracentrifugation, this interaction is too weak to cause binding of galactosyltransferase to ovalbumin-Sepharose. Sepharose 210-219 ovalbumin Bos taurus 21-30 932002-9 1976 While the binding of ovalbumin to galactosyltransferase in the presence of Mn2+ alone can be observed by ultracentrifugation, this interaction is too weak to cause binding of galactosyltransferase to ovalbumin-Sepharose. Sepharose 210-219 N-acetyllactosaminide alpha-1,3-galactosyltransferase Bos taurus 175-196 932002-9 1976 While the binding of ovalbumin to galactosyltransferase in the presence of Mn2+ alone can be observed by ultracentrifugation, this interaction is too weak to cause binding of galactosyltransferase to ovalbumin-Sepharose. Sepharose 210-219 ovalbumin Bos taurus 200-209 932002-10 1976 Binding to ovalbumin-Sepharose could only be detected by affinity chromatography in the presence of both Mn2+ (10 mM) and UDP (0.3 MM). Sepharose 21-30 ovalbumin Bos taurus 11-20 133010-1 1976 The reproducibility of a simplified, sensitive and rapid agarose-cell droplet assay for leucocyte migration inhibition factor (LIF) activity was studied. Sepharose 57-64 LIF interleukin 6 family cytokine Homo sapiens 88-125 986358-1 1976 Human acrosin was purified to electrophoretically homogeneous forms by acidic extraction of washed ejaculated spermatozoa and gel filtration of the acidic extracts on Sephadex G-75, followed by affinity chromatography on p-amino-benzamidine Sepharose. Sepharose 241-250 acrosin Homo sapiens 6-13 1088709-2 1976 Neuraminidase-treated lymphocytes were now fractionated on columns charged with large Sepharose particles to which HP had been coupled covalently. Sepharose 86-95 neuraminidase 1 Homo sapiens 0-13 5462-2 1976 By affinity chromatography, trypsins and chymotrypsins from mouse pancreas homogenates have been separated using soybean trypsin inhibitor immobilized on Sepharose. Sepharose 154-163 kunitz trypsin protease inhibitor Glycine max 121-138 5445-14 1976 The immunoglobin G fraction from these antisera completely inhibited lipoprotein lipase eluted from heparin-Sepharose columns. Sepharose 108-117 lipoprotein lipase Gallus gallus 69-87 942091-0 1976 Chromatography of renin substrate on concanavalin A--Agarose. Sepharose 53-60 renin Homo sapiens 18-23 945748-1 1976 Biospecific adsorbents which can be used for the purification of lectins are easily prepared by a one-step reaction between Epoxy-activated Sepharose 6 B and lectin-specific sugars. Sepharose 140-149 LOW QUALITY PROTEIN: lectin Glycine max 65-71 945748-2 1976 This paper reports the immobilization of N-acetyl-D-galactosamine and N-acetyl-D-glucosamine and the use of the resulting Sepharose derivatives for the purification of soybean lectin and wheat germ lectin, respectively. Sepharose 122-131 LOW QUALITY PROTEIN: lectin Glycine max 176-182 945748-2 1976 This paper reports the immobilization of N-acetyl-D-galactosamine and N-acetyl-D-glucosamine and the use of the resulting Sepharose derivatives for the purification of soybean lectin and wheat germ lectin, respectively. Sepharose 122-131 LOW QUALITY PROTEIN: lectin Glycine max 198-204 821479-6 1976 Rat serum galactosyltransferase was purified 6000-7000-fold by an affinity-chromatographic technique using a column of activated Sepharose 4B coupled with alpha-lactalbumin. Sepharose 129-138 glycoprotein alpha-galactosyltransferase 1 Rattus norvegicus 10-31 7245-11 1976 A rabbit anti-(human cathepsin G) serum was raised, and precipitin lines formed in agarose gel were stained for activity of the enzyme. Sepharose 83-90 cathepsin G Homo sapiens 21-32 1278164-0 1976 Binding of human hemoglobin and its polypeptide chains with haptoglobin coupled to an agarose matrix. Sepharose 86-93 haptoglobin Homo sapiens 60-71 1278164-1 1976 The interactions of human haptoglobin covalently linked to agarose with human hemoglobin and with p-chloromercuribenzoic-acid-treated alpha and beta chains (alpha* and beta* chains) were studied by flow chromatography and equilibrium binding. Sepharose 59-66 haptoglobin Homo sapiens 26-37 57803-1 1976 Two variants of alpha-fetoprotein in rat amniotic fluid were separated by their different affinity for concanavalin A-Sepharose, which selectively binds alpha-D-manno-pyranosides and alpha-D-glucopyranosides. Sepharose 118-127 alpha-fetoprotein Rattus norvegicus 16-33 1022286-2 1976 It is shown by two different methods (chromatography on Sepharose 46 and sucrose concentration gradient analysis) that the content of hexokinase polypeptides in stomach tumour is several times higher than in homologous normal tissue. Sepharose 56-65 hexokinase 1 Homo sapiens 134-144 1022290-1 1976 The ability of thrombin, immobilized on BrCN-activated Sepharose 4B, to split prothrombin, was studied. Sepharose 55-67 coagulation factor II Rattus norvegicus 15-23 1084890-2 1976 When purified antihemophilic factor (Factor VIII) was rechromatographed on 4% agarose in 0.15 M NaCl or 1.0 M NaCl, a single protein peak, containing both procoagulant activity and von Willebrand factor activity, as defined by ristocetin-induced platelet aggregation, was eluted in the void volume. Sepharose 78-85 coagulation factor VIII Homo sapiens 14-35 772186-8 1976 Platelets degramulated, but not aggregated, by exposure to TH in the presence of ethylene glycol bis(beta--aminoethyl ether)-N, N"-tetraacetic acid and plasmin were isolated by sepharose gel filtration. Sepharose 177-186 coagulation factor II, thrombin Homo sapiens 59-61 1254583-3 1976 Rhodopsin was extracted with detergents and purified by chromatographic procedures involving calcium phosphate/celite chromatography followed by affinity chromatograpy on concanavalin A-Sepharose (or in some cases, gel filtration on agarose). Sepharose 186-195 rhodopsin Bos taurus 0-9 1254583-3 1976 Rhodopsin was extracted with detergents and purified by chromatographic procedures involving calcium phosphate/celite chromatography followed by affinity chromatograpy on concanavalin A-Sepharose (or in some cases, gel filtration on agarose). Sepharose 233-240 rhodopsin Bos taurus 0-9 1260023-0 1976 Binding of bovine brain tissue factor to concanavalin A-Sepharose. Sepharose 56-65 LOC101909187 Bos taurus 24-37 1260023-2 1976 Tissue factor coagulant activity is adsorbed onto concanavalin A-Sepharose from sodium deoxycholate extracts of delipidated bovine brain powders. Sepharose 65-74 LOC101909187 Bos taurus 0-13 1254579-3 1976 A pure preparation of citrate synthase was obtained from a crude fraction of rat heart by the specific elution of the enzyme from the Sepharose-"ATP" with the dead end complex-forming substrates, oxalacetate and CoA. Sepharose 135-144 citrate synthase Rattus norvegicus 22-38 1260029-1 1976 Lipoprotein lipase (EC 3.1.1.3) from rat adipose tissue was purified by affinity chromatography with heparin-Sepharose. Sepharose 109-118 lipoprotein lipase Rattus norvegicus 0-18 1258905-2 1976 HPL was linked to Sepharose gel by cyanogen bromide activation and the resultant chromatographic resin was used in a repetitive column procedure to absorb and fractionate naturally occurring antibodies to HPL. Sepharose 18-27 galectin 1 Homo sapiens 0-3 182117-2 1976 Horse heart cytochrome c was covalently bound to Sepharose 4B and its redox properties were measured under various experimental conditions. Sepharose 49-58 cytochrome c, somatic Homo sapiens 12-24 947547-2 1976 One product of the in vitro synthesis was dihydrofolate reductase (DHFR), as confirmed by methotrexate-substituted Sepharose affinity chromatography followed by SDS-polyacrylamide slab gel electrophoresis and autoradiography of the proteins labeled with 35S-methionine. Sepharose 115-124 dihydrofolate reductase Mesocricetus auratus 67-71 179058-1 1976 Covalently closed relaxed SV40 DNA [SV40(I")] generated by polynucleotide ligase closure of nicked circular SV40 DNA was analyzed by agarose gel electrophoresis. Sepharose 133-140 DNA ligase 4 Homo sapiens 59-80 814122-1 1976 A rapid method is described for the purification of galactose-1-phosphate uridylyltransferase (EC 2.7.7.12) from human red blood cells by the use of DEAE-cellulose and two steps of affinity chromatography on a "uridine-aminohexyl" agarose column. Sepharose 231-238 galactose-1-phosphate uridylyltransferase Homo sapiens 52-93 773099-2 1976 Purified human C1q was covalently coupled to agarose or adsorbed to IgG-agarose resins. Sepharose 45-52 complement C1q A chain Homo sapiens 15-18 773099-2 1976 Purified human C1q was covalently coupled to agarose or adsorbed to IgG-agarose resins. Sepharose 72-79 complement C1q A chain Homo sapiens 15-18 773099-4 1976 Under conditions of antibody treatment which caused almost 100% inhibition of virus plaque formation, about 30% of formed 14C-labelled equine arteritis virus-antibody complexes was bound specifically to and desorbed from C1q-IgG agarose columns. Sepharose 229-236 complement C1q A chain Homo sapiens 221-224 6001-0 1976 An investigation of protein conformation of cytochrome c by using cytochrome c insolubilized on to agarose gel. Sepharose 99-106 cytochrome c, somatic Homo sapiens 44-56 6001-0 1976 An investigation of protein conformation of cytochrome c by using cytochrome c insolubilized on to agarose gel. Sepharose 99-106 cytochrome c, somatic Homo sapiens 66-78 6001-1 1976 Cytochrome c insolubilized on to agarose gel was shown to be more resistant to denaturation and carboxymethylation than the soluble protein. Sepharose 33-40 cytochrome c, somatic Homo sapiens 0-12 1275893-2 1976 After attachment to CNBr-activated Sepharose 4B, this derivative was able to remove glucokinase quantitatively from crude liver extracts and release it when the columns were developed with glucose, glucosamine, N-acetyl-glucosamine or KC1. Sepharose 35-44 glucokinase Rattus norvegicus 84-95 2779-1 1976 Partially purified rabbit liver UDPglucuronosyltransferase is immobilized on agarose by the cyanogen bromide activation method. Sepharose 77-84 UDP-glucuronosyltransferase 2C1 Oryctolagus cuniculus 32-58 2779-4 1976 The agarose-bound UDPglucuronosyltransferase is successfully used in the synthesis of p-nitrophenyl glucuronide in an overall yield of 50-70%. Sepharose 4-11 UDP-glucuronosyltransferase 2C1 Oryctolagus cuniculus 18-44 55436-4 1976 The suppressive activity is dose-dependent and absorbed by GAT-Sepharose, but not by BSA-Sepharose. Sepharose 63-72 glycine-N-acyltransferase Mus musculus 59-62 814163-3 1976 Antisera to C1r detected a protein of gamma-mobility on electrophoresis of serum in agarose in the presence of calcium, and a Beta-mobility protein when the electrophoretic separation was carried out in EDTA. Sepharose 84-91 complement C1r subcomponent Ovis aries 12-15 56053-0 1976 Synthesis of heparin-sepharoses and their binding with thrombin and antithrombin-heparin cofactor. Sepharose 21-31 coagulation factor II, thrombin Homo sapiens 55-63 1247582-5 1976 Individual fractions of thrombin-treated early fibrinogen digests isolated by agarose gel chromatography were treated with protamine sulfate at 37 degrees C resulting in precipitation-gelation of greater than 90 per cent of high molecular weight soluble fibrin complexes; whereas, less than 10 per cent of lower molecular weight fibrinogen degradation products precipitated by protamine sulfate. Sepharose 78-85 coagulation factor II, thrombin Homo sapiens 24-32 177230-1 1976 Complex formation in vitro between human alpha2-macroglobulin and the human proteases cationic trypsin, chymotrypsin, plasmin and granulocyte elastase and collagenase was clearly visualized by the use of thin-layer electrofocusing in polyacrylamide gel followed by electrophoresis in agarose gel containing antibodies against human alpha2-macroglobulin. Sepharose 284-291 alpha-2-macroglobulin Homo sapiens 41-61 177230-1 1976 Complex formation in vitro between human alpha2-macroglobulin and the human proteases cationic trypsin, chymotrypsin, plasmin and granulocyte elastase and collagenase was clearly visualized by the use of thin-layer electrofocusing in polyacrylamide gel followed by electrophoresis in agarose gel containing antibodies against human alpha2-macroglobulin. Sepharose 284-291 plasminogen Homo sapiens 118-125 13595-1 1976 Two papain inhibitors, I1 and I2, from rat skin extract were purified by affinity chromatography on KSCN-modified papain-agarose gel and by gel filtration on Sephadex G-100. Sepharose 121-128 protein phosphatase 1, regulatory (inhibitor) subunit 1A Rattus norvegicus 23-32 1015477-0 1976 Studies of transferrin polymorphism in Swedish cattle using agarose gel electrophoresis. Sepharose 60-67 serotransferrin Bos taurus 11-22 137671-5 1976 In in vitro studies, after treatment of plasma with thrombin or plasmin, factor VIII activity was lost, whereas the amount of factor VIII-related antigen remained the same or was even increased when measured by agarose quantitative immunoelectrophoresis. Sepharose 211-218 cytochrome c oxidase subunit 8A Homo sapiens 133-137 2535-2 1976 A 3alpha-hydroxysteroid dehydrogenase is purified about 100-fold by double affinity chromatography on 5alpha-dihydrotestosterone-Sepharose. Sepharose 129-138 aldo-keto reductase family 1, member C14 Rattus norvegicus 2-37 814097-0 1976 Measurement of antibody to influenza virus neuraminidase by single radial hemolysis in agarose gels. Sepharose 87-94 neuraminidase 1 Homo sapiens 43-56 1249429-1 1976 Human serum albumin-Sepharose was prepared by coupling human serum albumin to cyanogen bromide activated Sepharose 4B. Sepharose 20-29 albumin Homo sapiens 6-19 1249429-1 1976 Human serum albumin-Sepharose was prepared by coupling human serum albumin to cyanogen bromide activated Sepharose 4B. Sepharose 20-29 albumin Homo sapiens 61-74 1249429-1 1976 Human serum albumin-Sepharose was prepared by coupling human serum albumin to cyanogen bromide activated Sepharose 4B. Sepharose 105-114 albumin Homo sapiens 6-19 778275-2 1976 Human leucocyte migration inhibitory factor (LIF) was subjected to affinity column chromatography on Sepharose-bound alpha-L-fucose. Sepharose 101-110 LIF interleukin 6 family cytokine Homo sapiens 6-43 778275-2 1976 Human leucocyte migration inhibitory factor (LIF) was subjected to affinity column chromatography on Sepharose-bound alpha-L-fucose. Sepharose 101-110 LIF interleukin 6 family cytokine Homo sapiens 45-48 24424759-6 1976 Agarose gel electrophoresis of GA3-protein complexes reveals two zones of radioactivity at the anodic part of the electrophoretogram. Sepharose 0-7 succinyl-CoA:glutarate-CoA transferase Homo sapiens 31-34 59924-3 1976 The anti-renin globulins were coupled to cyanogen bromide activated Sepharose. Sepharose 68-77 renin Canis lupus familiaris 9-14 59924-4 1976 Using the anti-renin globulin-coupled Sepharose as an immuno-adsorbant, a method was devised allowing purification of plasma renin to a 1,000-fold purity. Sepharose 38-47 renin Canis lupus familiaris 15-20 59924-4 1976 Using the anti-renin globulin-coupled Sepharose as an immuno-adsorbant, a method was devised allowing purification of plasma renin to a 1,000-fold purity. Sepharose 38-47 renin Canis lupus familiaris 125-130 65003-0 1976 Affinity chromatography of beta2-microglobulin from human lymphocytes on concanavalin-A-sepharose. Sepharose 88-97 beta-2-microglobulin Homo sapiens 27-46 65003-2 1976 Beta2-microglobulin was extracted from human lymphocytes with nonionic detergent and separated by affinity chromatography on concanavalin-A-Sepharose. Sepharose 140-149 beta-2-microglobulin Homo sapiens 0-19 822509-4 1976 Virtually all the antinuclear factor (ANF) activity of this serum was extracted by IgG-agarose. Sepharose 87-94 natriuretic peptide A Homo sapiens 38-41 1013647-1 1976 By affinity chromatography on Sepharose columns containing insolubilized ricinus agglutinin, all immunoglobulins of the IgA2 subclass and the polymers of IgA1 were retained and could be eluted with lactose. Sepharose 30-39 immunoglobulin heavy constant alpha 1 Homo sapiens 154-158 1014940-0 1976 [Preparation of pure albumin and transferrin from rabbit serum using con A-sepharose]. Sepharose 75-84 transferrin Homo sapiens 33-44 1084-2 1975 This purified FABP represents two protein bands that bind PGA on polyacrylamide disc gel electrophoreis, elutes from DEAE-cellulose in 0.001 M phosphate buffer, stains positive with PAS, Elutes from concanavalin A Sepharose affinity columns with methyl alpha-mannoside, and shows three major peaks (pl =6.8, 7.5, 8.2) by isotric focusing. Sepharose 214-223 folate receptor alpha Homo sapiens 14-18 53072-5 1975 Anti-beta-glucuronidase inhibitor isolated from human serum, by fractionation with (NH4)2 SO4 followed by DEAE-cellulose, Sephadex G-200 and Sepharose 4B chromatography, was identified as alpha2-macroglobulin by using ultracentrifuge analysis and immunoelectrophoresis. Sepharose 141-150 LOW QUALITY PROTEIN: alpha-2-macroglobulin Oryctolagus cuniculus 188-208 811661-5 1975 We have purified vitellogenin to hemogeneity by two methods: chromatography on diethylaminoethyl-52-cellulose and affinity chromatography on an antibody-Sepharose column. Sepharose 153-162 vitellogenin Oryctolagus cuniculus 17-29 1217705-0 1975 Albumin immobilized on agarose as a tool for measuring ligand binding of proteins or peptides. Sepharose 23-30 albumin Homo sapiens 0-7 56178-6 1975 Brain Thy-1 was further purified by affinity chromatography with lentil lectin coupled to Sepharose 4B, and more than 80% of the antigen was bound. Sepharose 90-99 Thy-1 cell surface antigen Rattus norvegicus 6-11 175899-2 1975 SBI covalently cross-linked to Sepharose beads inhibited the MLR and mitogen stimulation virtually completely. Sepharose 31-40 cysteine rich secretory protein LCCL domain containing 2 Homo sapiens 0-3 1204646-2 1975 Dihydrofolate reductase from bovine liver has been purified 5000-fold employing conventional techniques and methotrexate/aminohexyl/Sepharose affinity chromatography. Sepharose 132-141 dihydrofolate reductase Bos taurus 0-23 1239396-4 1975 Insulin antibodies coupled to Sepharose were used to bind human proinsulin and insulin in the serum and after centrifugation C-peptide was determined in the supernatant. Sepharose 30-39 insulin Homo sapiens 64-74 1239396-4 1975 Insulin antibodies coupled to Sepharose were used to bind human proinsulin and insulin in the serum and after centrifugation C-peptide was determined in the supernatant. Sepharose 30-39 insulin Homo sapiens 67-74 810515-8 1975 The crude glycoprotein fraction from the agarose column was resolved into the three major components, GP1, 2, and 3, distinguished by their isoelectric points (pI 3.9, 4.4, and 5.0, respectively), electrophoretic mobilities at pH 8.6, and reactivities with antiserum in immunoelectrophoresis. Sepharose 41-48 pancreatic secretory granule membrane major glycoprotein GP2 Cavia porcellus 102-115 811690-6 1975 TRH was eluted from the anti-TRH Sepharose with acetic acid and quantitated by radioimmunoassay of the lyophilized acetic acid eluate. Sepharose 33-42 thyrotropin releasing hormone Rattus norvegicus 0-3 811690-6 1975 TRH was eluted from the anti-TRH Sepharose with acetic acid and quantitated by radioimmunoassay of the lyophilized acetic acid eluate. Sepharose 33-42 thyrotropin releasing hormone Rattus norvegicus 29-32 812922-2 1975 Moderately purified C1q is obtained by dialyzing fresh human serum in the presence of chelating agents at low ionic strength and then electrophoresing it in agarose. Sepharose 157-164 complement C1q A chain Homo sapiens 20-23 1082142-1 1975 Biological activities associated with colony-stimulating factor (CSF) from human leukemic urine were found to be selectively retained on an affinity adsorbent of Con A-Sepharose. Sepharose 168-177 colony stimulating factor 2 Homo sapiens 38-63 1082142-1 1975 Biological activities associated with colony-stimulating factor (CSF) from human leukemic urine were found to be selectively retained on an affinity adsorbent of Con A-Sepharose. Sepharose 168-177 colony stimulating factor 2 Homo sapiens 65-68 1082142-5 1975 Affinity chromatography of CSF on a Blue Dextran-Sepharose adsorbent was found to be an effective method for removing albumin, a major protein contaminant in urinary preparations. Sepharose 49-58 colony stimulating factor 2 Homo sapiens 27-30 416-1 1975 Human glucose 6-phosphate dehydrogenase associated with NADPH was efficiently bound with agarose-bound NADP, whereas the enzyme associated with NADP was poorly bound with agarose-bound NADP. Sepharose 89-96 glucose-6-phosphate dehydrogenase Homo sapiens 6-39 416-1 1975 Human glucose 6-phosphate dehydrogenase associated with NADPH was efficiently bound with agarose-bound NADP, whereas the enzyme associated with NADP was poorly bound with agarose-bound NADP. Sepharose 171-178 glucose-6-phosphate dehydrogenase Homo sapiens 6-39 72-4 1975 Polyacrylamide gel electrophoresis of polypeptides released from denatured Sepharose-Myosin indicates that 85% of the myosin is attached to the agarose beads through the heavy chains and the remainder through the light chains, in agreement with predictions of binding and release based upon either the lysine contents or molecular weights of themyosin subunits. Sepharose 75-84 myosin heavy chain 14 Homo sapiens 85-91 72-4 1975 Polyacrylamide gel electrophoresis of polypeptides released from denatured Sepharose-Myosin indicates that 85% of the myosin is attached to the agarose beads through the heavy chains and the remainder through the light chains, in agreement with predictions of binding and release based upon either the lysine contents or molecular weights of themyosin subunits. Sepharose 75-84 myosin heavy chain 14 Homo sapiens 118-124 72-4 1975 Polyacrylamide gel electrophoresis of polypeptides released from denatured Sepharose-Myosin indicates that 85% of the myosin is attached to the agarose beads through the heavy chains and the remainder through the light chains, in agreement with predictions of binding and release based upon either the lysine contents or molecular weights of themyosin subunits. Sepharose 144-151 myosin heavy chain 14 Homo sapiens 85-91 72-4 1975 Polyacrylamide gel electrophoresis of polypeptides released from denatured Sepharose-Myosin indicates that 85% of the myosin is attached to the agarose beads through the heavy chains and the remainder through the light chains, in agreement with predictions of binding and release based upon either the lysine contents or molecular weights of themyosin subunits. Sepharose 144-151 myosin heavy chain 14 Homo sapiens 118-124 1189627-0 1975 Transferrin variants designated by their relative mobilities in high-voltage agarose gel electrophoresis. Sepharose 77-84 transferrin Homo sapiens 0-11 1189627-1 1975 A unified nomenclature for the designation of transferrin variants, based on their relative mobilities in a standardized high-voltage agarose gel electrophoresis, is proposed. Sepharose 134-141 transferrin Homo sapiens 46-57 52420-1 1975 Human alpha-fetoprotein (AFP) was isolated from cord serum on an immunoadsorbent column obtained by covalently linking rabbit anti AFP to cyanogen bromide activated Sepharose. Sepharose 165-174 alpha fetoprotein Homo sapiens 6-23 52420-1 1975 Human alpha-fetoprotein (AFP) was isolated from cord serum on an immunoadsorbent column obtained by covalently linking rabbit anti AFP to cyanogen bromide activated Sepharose. Sepharose 165-174 alpha fetoprotein Homo sapiens 25-28 1201147-1 1975 Rat heart lipoprotein lipase was highly purified by affinity chromatography using heparin-Sepharose 4B. Sepharose 90-102 lipase G, endothelial type Rattus norvegicus 22-28 52402-0 1975 Demonstration of two molecular variants of carcinoembryonic antigen by concanavalin A sepharose affinity chromatography. Sepharose 86-95 CEA cell adhesion molecule 3 Homo sapiens 43-67 52402-1 1975 The carcinoembryonic antigen (CEA) active glycoproteins from perchloric acid extract of liver-metastasized primary colon tumor have been separated by concanavalin A Sepharose (Con A Sepharose) chromatography. Sepharose 165-174 CEA cell adhesion molecule 3 Homo sapiens 4-28 52402-1 1975 The carcinoembryonic antigen (CEA) active glycoproteins from perchloric acid extract of liver-metastasized primary colon tumor have been separated by concanavalin A Sepharose (Con A Sepharose) chromatography. Sepharose 165-174 CEA cell adhesion molecule 3 Homo sapiens 30-33 52402-1 1975 The carcinoembryonic antigen (CEA) active glycoproteins from perchloric acid extract of liver-metastasized primary colon tumor have been separated by concanavalin A Sepharose (Con A Sepharose) chromatography. Sepharose 182-191 CEA cell adhesion molecule 3 Homo sapiens 4-28 52402-1 1975 The carcinoembryonic antigen (CEA) active glycoproteins from perchloric acid extract of liver-metastasized primary colon tumor have been separated by concanavalin A Sepharose (Con A Sepharose) chromatography. Sepharose 182-191 CEA cell adhesion molecule 3 Homo sapiens 30-33 52402-2 1975 The CEA activities separated by Con A Sepharose chromatography were designated as loosely bound and tightly bound which, respectively, eluted on the Con A Sepharose column between 0.12 and 0.15 M and 0.3 M alpha-methylmannose in a linear gradient of alpha-methylmannose. Sepharose 38-47 CEA cell adhesion molecule 3 Homo sapiens 4-7 52402-2 1975 The CEA activities separated by Con A Sepharose chromatography were designated as loosely bound and tightly bound which, respectively, eluted on the Con A Sepharose column between 0.12 and 0.15 M and 0.3 M alpha-methylmannose in a linear gradient of alpha-methylmannose. Sepharose 155-164 CEA cell adhesion molecule 3 Homo sapiens 4-7 241482-3 1975 Antibody to this preparation was rendered specific for CEA by adsorption on a column of normal liver proteins bound to Sepharose. Sepharose 119-128 CEA cell adhesion molecule 3 Homo sapiens 55-58 1182698-1 1975 Using exclusion from Sepharose 4B as our criterion, we have found a high-molecular-weight form of alkaline phosphatase and of leucine aminopeptidase which are released into the culture media by the FL amnion cell line. Sepharose 21-30 carboxypeptidase Q Homo sapiens 134-148 1204472-0 1975 [Activation of prothrombin by thrombin immobilized on agarose]. Sepharose 54-61 coagulation factor II, thrombin Homo sapiens 15-26 1204472-0 1975 [Activation of prothrombin by thrombin immobilized on agarose]. Sepharose 54-61 coagulation factor II, thrombin Homo sapiens 18-26 1108037-9 1975 Maximal detection of plaques with Mrbc was observed when human complement was used in assays containing agarose. Sepharose 104-111 MRBC Homo sapiens 34-38 810169-0 1975 Chromatographic purification of a mammalian histidine decarboxylase on charged and non-charged alkyl derivatives of agarose. Sepharose 116-123 histidine decarboxylase Homo sapiens 44-67 810169-1 1975 Histidine decarboxylase (EC 4.1.1.22) from a mouse mastocytoma has been purified by chromatography on charged and non-charged n-alkyl derivatives of agarose. Sepharose 149-156 histidine decarboxylase Mus musculus 0-23 810169-8 1975 The histidine decarboxylase, thus purified, retained 90-100% of its activity for 10 days or more at 6-8 degrees C. Some general comments on protein fractionation on charged and non-charged alkyl derivatives of agarose are given. Sepharose 210-217 histidine decarboxylase Homo sapiens 4-27 1243914-6 1975 The purification of anti-Tg was performed by affinity chromatography using Tg-Sepharose, as an immunoadsorbent. Sepharose 78-87 thyroglobulin Homo sapiens 25-27 1243914-6 1975 The purification of anti-Tg was performed by affinity chromatography using Tg-Sepharose, as an immunoadsorbent. Sepharose 78-87 thyroglobulin Homo sapiens 75-77 1243914-8 1975 The immunoadsorbent (Tg-Sepharose conjugate) was used in a column procedure for the isolation of anti-Tg from globulin fractions obtained from Hashimoto"s sera. Sepharose 24-33 thyroglobulin Homo sapiens 21-23 1243914-8 1975 The immunoadsorbent (Tg-Sepharose conjugate) was used in a column procedure for the isolation of anti-Tg from globulin fractions obtained from Hashimoto"s sera. Sepharose 24-33 thyroglobulin Homo sapiens 102-104 1243915-18 1975 From these observations, it is concluded that MIF against Tg can easily be detected by the agarose plate technique and that cellular immunity may play a more important role in the initial phase of autoimmune thyroid diseases than the later phase, in which the serum levels of circulating antibodies are becoming predominant. Sepharose 91-98 macrophage migration inhibitory factor Homo sapiens 46-49 1243915-18 1975 From these observations, it is concluded that MIF against Tg can easily be detected by the agarose plate technique and that cellular immunity may play a more important role in the initial phase of autoimmune thyroid diseases than the later phase, in which the serum levels of circulating antibodies are becoming predominant. Sepharose 91-98 thyroglobulin Homo sapiens 58-60 812485-1 1975 The alpha-galactosidase A activity from fibroblasts of five Fabry patients and five controls has been separated from alpha-galactosidase B through small DEAE-cellulose columns and in some experiments by treatment of the fibroblast extracts with Sepharose coupled to anti-alpha-galactosidase B antibodies. Sepharose 245-254 galactosidase alpha Homo sapiens 4-25 53066-1 1975 Human and bovine antithrombin II/III have been isolated by a simple procedure essentially using only affinity chromatography on heparin-agarose and polyethylene glycol precipitation. Sepharose 136-143 serpin family C member 1 Homo sapiens 17-36 1182179-2 1975 Conditions are described in which Val-, Trp-, Phe-, Leu- and Ile-tRNA synthetases from yellow lupin seeds can be separated from each other on the tRNA-Sepharose columns. Sepharose 151-160 5'-nucleotidase, cytosolic IIIA Homo sapiens 94-99 765256-3 1975 The amino acid composition of [alpha1 (III)]3 purified by CM-cellulose chromatography and agarose-gel chromatography includes two cysteine residues per chain, and the hydroxyproline/proline ratio is greater than 1.0. Sepharose 90-97 adrenoceptor alpha 1D Homo sapiens 30-45 1080491-5 1975 In one patient the Factor VIII/von Willebrand factor protein eluted from Sepharose 4B in a position and distribution identical to normal with normal levels of procoagulant activity and antigen. Sepharose 73-82 von Willebrand factor Homo sapiens 31-52 1080491-7 1975 In the second patient the peak of Factor VIII/von Willebrand factor protein, antigen, and procoagulant activity eluted from a Sepharose 4B column with an estimated molecular weight of approximately half that of normal. Sepharose 126-135 von Willebrand factor Homo sapiens 46-67 1081233-6 1975 In some peaks, only one or two protein bands are observed, suggesting that affinity chromatography on Sepharose- or Affi-Gel 10-chymotrypsin might be used for the isolation of alpha1-antitrypsin and other inhibitors in preparative amounts. Sepharose 102-111 serpin family A member 1 Homo sapiens 176-194 1158896-1 1975 Preparative agarose gel electrophoresis under denaturing conditions has been successfully employed to purify large quantities of ovalbumin mRNA from hen oviducts. Sepharose 12-19 ovalbumin (SERPINB14) Gallus gallus 129-138 51601-2 1975 Monospecificity of the antiserums for the gamma-, alpha-, and mu-chains was obtained by absorbing them in agarose-linked immunosorbent columns. Sepharose 106-113 Fc gamma receptor and transporter Homo sapiens 42-71 51601-4 1975 Contaminating anti-alpha2-macroglobulin antibodies in the anti-IgA and anti-IgM serums were removed with agarose-linked fetal globulins. Sepharose 105-112 alpha-2-macroglobulin Homo sapiens 19-39 1239278-2 1975 Lipoprotein lipase was purified from pig myocardium by a two-step purification procedure involving (a) the formation of an enzyme-substrate complex and (b) affinity chromatography on Sepharose which contained covalently linked heparin. Sepharose 183-192 lipoprotein lipase Sus scrofa 0-18 170110-22 1975 Sepharose-bound Ap5A was used for removing traces of adenylate kinase from samples of myosin and creatine kinase. Sepharose 0-9 myosin heavy chain 14 Homo sapiens 86-92 809280-7 1975 A Simple method developed for the isolation of human alpha1-antitrypsin was equally effective for the various inherited phenotypes and for alpha1-antitrypsin from the dog, baboon, and monkey, Glutathione-Sepharose was also used successfully, but the nu-chain conjugate yielded alpha1-antitrypsin less contaminated with mercaptalbumin and prealbumin. Sepharose 204-213 serpin family A member 1 Canis lupus familiaris 53-71 170205-1 1975 Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Sepharose 51-60 cpe Clostridium perfringens 5-16 170205-1 1975 Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Sepharose 51-60 cpe Clostridium perfringens 157-168 170205-1 1975 Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Sepharose 69-78 cpe Clostridium perfringens 5-16 170205-1 1975 Anti-enterotoxin immunoglobulins immobilized on CH-Sepharose or CNBr-Sepharose were used for affinity chromatography purification of Clostridium perfringens enterotoxin. Sepharose 69-78 cpe Clostridium perfringens 157-168 1102408-2 1975 The biosynthetic rate was quantitated as the incorporation of 3H-leucine into proinsulin and insulin, which were separated from the crude islet homogenates with anti-insulin serum coupled to cyanogen bromide activated Sepharose 4B. Sepharose 218-227 insulin II Mus musculus 78-88 1080490-1 1975 When human, canine, or bovine factor VIII preparations are chromatographed on 4% agarose at ionic strength 0.2, the factor VIII activity elutes as a single peak in the void volume with slight tailing. Sepharose 81-88 coagulation factor VIII Bos taurus 30-41 1080490-1 1975 When human, canine, or bovine factor VIII preparations are chromatographed on 4% agarose at ionic strength 0.2, the factor VIII activity elutes as a single peak in the void volume with slight tailing. Sepharose 81-88 coagulation factor VIII Bos taurus 116-127 1225911-1 1975 An immunoadsorbent-antibody (egg albumin-Sepharose-antibody) column was found to be suitable for the rapid separation of C1-esterase from normal human serum. Sepharose 41-50 complement C1s Homo sapiens 121-132 1059123-4 1975 The messenger RNA that specifies vitellogenin has been purified more than 800-fold from rooster liver polysomal RNA by a combination of methods, including immunoprecipitation of polysomes and chromatography of RNA on poly(U)-Sepharose. Sepharose 225-234 putative uncharacterized protein LOC400499 Homo sapiens 33-45 52179-1 1975 The use of Sepharose-conjugated murine anti-Iga or anti-Igb allo-antisera allowed the quantitative separation of immunoglobulins of the two allotypes. Sepharose 11-20 immunoglobulin heavy constant alpha Mus musculus 44-47 169249-10 1975 The binding activity is lost when the solubilized thyrotropin receptor preparation is exposed to beads of neuraminidase-Sepharose or conconavalin A-Sepharose. Sepharose 120-129 thyroid stimulating hormone receptor Bos taurus 50-70 169249-10 1975 The binding activity is lost when the solubilized thyrotropin receptor preparation is exposed to beads of neuraminidase-Sepharose or conconavalin A-Sepharose. Sepharose 148-157 thyroid stimulating hormone receptor Bos taurus 50-70 808238-2 1975 The procedure is based upon the binding of histaminase to cadaverine, a diamine substrate for the enzyme, which is coupled to Sepharose. Sepharose 126-135 amine oxidase copper containing 1 Homo sapiens 43-54 1158872-2 1975 CI globulin was distinguished from antihemophilic factor (AHF) by amino acid analysis, position of elution from 4% agarose, and electrophoretic migration in polyacrylamide gels in the presence of sodium dodecyl sulfate without prior reduction. Sepharose 115-122 fibronectin 1 Homo sapiens 0-11 168889-0 1975 Purification of cytochrome oxidase by using Sepharose-bound cytochrome c. Sepharose 44-53 cytochrome c, somatic Homo sapiens 60-72 240517-0 1975 The heterogeneity of the serum activity of gamma-glutamyl transpeptidase in hepatobiliary diseases as studied by agarose gel electrophoresis. Sepharose 113-120 inactive glutathione hydrolase 2 Homo sapiens 43-72 50707-3 1975 Multiple discrete IgG bands were demonstrated by agarose electrophoresis in serum and in CSF during the first 2 weeks after onset of the neurological symptoms, and also after week 2 and still during week 12 after onset. Sepharose 49-56 colony stimulating factor 2 Homo sapiens 89-92 241335-1 1975 Actin and myosin were immobilized by coupling them to agarose matrices. Sepharose 54-61 myosin heavy chain 14 Homo sapiens 10-16 241335-4 1975 Sepharose-G-actin columns were used to bind heavy meromyosin and myosin subfragment 1 specifically and reversibly. Sepharose 0-9 myosin heavy chain 14 Homo sapiens 54-60 241335-8 1975 The complexes formed between the myosin derivatives and Sepharose-G-actin can be dissociated by low concentrations of ATP, ADP and pyrophosphate in both the presence and the absence of Mg2+. Sepharose 56-65 myosin heavy chain 14 Homo sapiens 33-39 1201740-2 1975 Human thyroglobulin was purified by combined DEAE-cellulose and affinity chromatography using Sepharose 4B-bound Concanavalin A. Sepharose 94-106 thyroglobulin Homo sapiens 6-19 1079850-3 1975 Purification of IBF by affinity chromatography on IgG-coated Sepharose columns led to an increase of the suppressive capacity with IgG, IgM, or Fab2 from IgG) the factor responsible for inhibiting the PFC response could not be dissociated from that responsible for the inhibitory activity of IBF on C-dependent hemolysis. Sepharose 61-70 immunoglobulin heavy variable V1-62 Mus musculus 50-53 1079850-3 1975 Purification of IBF by affinity chromatography on IgG-coated Sepharose columns led to an increase of the suppressive capacity with IgG, IgM, or Fab2 from IgG) the factor responsible for inhibiting the PFC response could not be dissociated from that responsible for the inhibitory activity of IBF on C-dependent hemolysis. Sepharose 61-70 immunoglobulin heavy variable V1-62 Mus musculus 131-134 1079850-3 1975 Purification of IBF by affinity chromatography on IgG-coated Sepharose columns led to an increase of the suppressive capacity with IgG, IgM, or Fab2 from IgG) the factor responsible for inhibiting the PFC response could not be dissociated from that responsible for the inhibitory activity of IBF on C-dependent hemolysis. Sepharose 61-70 immunoglobulin heavy variable V1-62 Mus musculus 131-134 1100756-3 1975 Lysine-vasopressin (LVP), arginine-vasopressin (AVP) or oxytocin were covalently bound to CNB-activated agarose beads. Sepharose 104-111 arginine vasopressin Rattus norvegicus 7-18 1100756-3 1975 Lysine-vasopressin (LVP), arginine-vasopressin (AVP) or oxytocin were covalently bound to CNB-activated agarose beads. Sepharose 104-111 arginine vasopressin Rattus norvegicus 26-52 807640-3 1975 Affinity chromatography of serum-EDTA on Sepharose-IgG resulted in binding of only C1q and C1r. Sepharose 41-50 complement C1q A chain Homo sapiens 83-86 807640-3 1975 Affinity chromatography of serum-EDTA on Sepharose-IgG resulted in binding of only C1q and C1r. Sepharose 41-50 complement C1r Homo sapiens 91-94 807640-5 1975 By the use of purified C1 proteins and Sepharose-IgG in binding studies it was confirmed that both C1q and C1r bind independently to sites on IgG and hold C1s and C1t by Ca++-dependent bonds. Sepharose 39-48 complement C1q A chain Homo sapiens 99-102 807640-5 1975 By the use of purified C1 proteins and Sepharose-IgG in binding studies it was confirmed that both C1q and C1r bind independently to sites on IgG and hold C1s and C1t by Ca++-dependent bonds. Sepharose 39-48 complement C1r Homo sapiens 107-110 807640-9 1975 The evidence suggests that the spatial sequence of the components of the Sepharose-IgG-Serum C1 complex is: Sepharose-IgG: C1q: C1t: C1s: C1r: IgG-Sepharose. Sepharose 73-82 complement C1q A chain Homo sapiens 123-126 807640-9 1975 The evidence suggests that the spatial sequence of the components of the Sepharose-IgG-Serum C1 complex is: Sepharose-IgG: C1q: C1t: C1s: C1r: IgG-Sepharose. Sepharose 73-82 complement C1r Homo sapiens 138-141 807640-9 1975 The evidence suggests that the spatial sequence of the components of the Sepharose-IgG-Serum C1 complex is: Sepharose-IgG: C1q: C1t: C1s: C1r: IgG-Sepharose. Sepharose 108-117 complement C1q A chain Homo sapiens 123-126 807640-9 1975 The evidence suggests that the spatial sequence of the components of the Sepharose-IgG-Serum C1 complex is: Sepharose-IgG: C1q: C1t: C1s: C1r: IgG-Sepharose. Sepharose 108-117 complement C1r Homo sapiens 138-141 807640-9 1975 The evidence suggests that the spatial sequence of the components of the Sepharose-IgG-Serum C1 complex is: Sepharose-IgG: C1q: C1t: C1s: C1r: IgG-Sepharose. Sepharose 108-117 complement C1q A chain Homo sapiens 123-126 807640-9 1975 The evidence suggests that the spatial sequence of the components of the Sepharose-IgG-Serum C1 complex is: Sepharose-IgG: C1q: C1t: C1s: C1r: IgG-Sepharose. Sepharose 108-117 complement C1r Homo sapiens 138-141 124734-15 1975 The macrophage actin-binding protein and rabbit erythrocyte spectrin eluted from agarose columns with a KAV of 0.24 and in the excluded volumes. Sepharose 81-88 actin Oryctolagus cuniculus 15-20 1170897-2 1975 Three different data analyses of the results of gel chromatography in the presence of 6 M guanidine-HCl on 4% agarose give values of 159 000, 161 000, and 165 000 for amylo-1,6-glucosidase/4-alpha-glucanotransferase from rabbit muscle. Sepharose 110-117 glycogen debranching enzyme Oryctolagus cuniculus 167-215 1170885-0 1975 Growth hormone covalently bound to sepharose or glass. Sepharose 35-44 growth hormone 1 Homo sapiens 0-14 1170885-2 1975 Purified boveine growth hormone labeled enzymatically with iodine-125 was covalently coupled to cyanogen bromide activated Sepharose 4B gel and to diazotized zirconia-clad glass beads. Sepharose 123-135 growth hormone 1 Homo sapiens 17-31 1201212-2 1975 Mixing various quantities of heparin with agarose in the first phase of electrophoresis, AT-III from normal human plasma and serum revealed a heterogeneity which depended on the heparin concentration in the agarose gel. Sepharose 42-49 serpin family C member 1 Homo sapiens 89-95 1201212-2 1975 Mixing various quantities of heparin with agarose in the first phase of electrophoresis, AT-III from normal human plasma and serum revealed a heterogeneity which depended on the heparin concentration in the agarose gel. Sepharose 207-214 serpin family C member 1 Homo sapiens 89-95 1201212-10 1975 It is concluded that high molecular weight complexes between AT-III and activated coagulation factors may be present in normally circulating blood and that their detection and possibly quantitation can be achieved using the heparin/agarose crossed immunoelectrophoresis system. Sepharose 232-239 serpin family C member 1 Homo sapiens 61-67 1213264-4 1975 Gel filtration (4% agarose) of the redissolved precipitate resulted in a separation of SFMC and fibrinogen. Sepharose 19-26 fibrinogen beta chain Homo sapiens 96-106 807868-9 1975 For a laboratory with special interest in multiple sclerosis, agarose gel-electrophoresis of concentrated CSF appears to be the test of choice. Sepharose 62-69 colony stimulating factor 2 Homo sapiens 106-109 125463-6 1975 in the binding studies with the highly purified plasminogen and TLCK-plasmin preparations which were obtained by affinity chromatography on lysine-substituted Sepharose, the molar binding ratio was shown to be 1.5-1.6 moles tranexamic acid per one mole protein. Sepharose 159-168 plasminogen Homo sapiens 48-55 126576-9 1975 C3 was activated and the resulting C3b fragment fixed on agarose by incubating C3 with trypsin covalently bound to the agarose. Sepharose 57-64 complement C3 Homo sapiens 35-38 126576-9 1975 C3 was activated and the resulting C3b fragment fixed on agarose by incubating C3 with trypsin covalently bound to the agarose. Sepharose 119-126 complement C3 Homo sapiens 35-38 126576-10 1975 The agarose-C3b intermediate was capable of binding GBG provided Mg++ was present. Sepharose 4-11 complement C3 Homo sapiens 12-15 1101767-6 1975 This antiovalbumin was not bound directly to Sepharose but to Sepharose-coupled ovalbumin. Sepharose 45-54 serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene Mus musculus 9-18 1101767-6 1975 This antiovalbumin was not bound directly to Sepharose but to Sepharose-coupled ovalbumin. Sepharose 62-71 serine (or cysteine) peptidase inhibitor, clade B, member 1, pseudogene Mus musculus 9-18 1154312-2 1975 When agarose was applied in the first phase of the crossed immunoelectrophoresis, the normal and the pathological AT-III revealed identical electrophoretic mobility. Sepharose 5-12 serpin family C member 1 Homo sapiens 114-120 1154312-3 1975 However, when heparin was mixed with agarose in the first phase of electrophoresis, the propositus" plasma displayed a different AT-III pattern from normal plasma. Sepharose 37-44 serpin family C member 1 Homo sapiens 129-135 807251-1 1975 Intrinsic as well as extrinsic fluorescence of an immobilized protein was used for the study of the interactions between alpha-lactalbumin-Sepharose and protein ligands. Sepharose 139-148 lactalbumin alpha Homo sapiens 121-138 807251-2 1975 The fluorescence peak of the human alpha-lactalbumin-agarose conjugate was shifted to the blue and quenched in the presence of the galactosyl transferase (A-protein), indicating the probable formation of a complex between both proteins. Sepharose 53-60 lactalbumin alpha Homo sapiens 35-52 807251-3 1975 The natural fluorescence of human alpha-lactalbumin bound to Sepharose was specifically quenched in presence of antihuman alpha-lactalbumin antibodies. Sepharose 61-70 lactalbumin alpha Homo sapiens 34-51 807251-3 1975 The natural fluorescence of human alpha-lactalbumin bound to Sepharose was specifically quenched in presence of antihuman alpha-lactalbumin antibodies. Sepharose 61-70 lactalbumin alpha Homo sapiens 122-139 1138860-6 1975 Whereas treatment of Con A- (and insulin-) derivatized beads with anti-insulin antiserum, and cells with trypsin, readily inhibited binding of insulin-Sepharose to cells, neither treatment inhibited Con A-Sepharose binding. Sepharose 151-160 insulin Homo sapiens 33-40 1138860-6 1975 Whereas treatment of Con A- (and insulin-) derivatized beads with anti-insulin antiserum, and cells with trypsin, readily inhibited binding of insulin-Sepharose to cells, neither treatment inhibited Con A-Sepharose binding. Sepharose 151-160 insulin Homo sapiens 71-78 1138860-6 1975 Whereas treatment of Con A- (and insulin-) derivatized beads with anti-insulin antiserum, and cells with trypsin, readily inhibited binding of insulin-Sepharose to cells, neither treatment inhibited Con A-Sepharose binding. Sepharose 151-160 insulin Homo sapiens 71-78 1181266-1 1975 The acetylcholinesterase from human erythrocytes was released from the plasma membrane with 0.2% Triton X-100 at low ionic strength and purified by two affinity chromatography steps on Sepharose-bound m-[6-(6-amino-caproylamino)caproylamino]phenyltrimethyl-ammonium. Sepharose 185-194 acetylcholinesterase (Cartwright blood group) Homo sapiens 4-24 1169259-1 1975 Monovalent goat antibody fragments (Fab) that were monospecific for human fibrinogen were isolated by affinity chromatography on fibrinogen-Sepharose and used as a direct probe for the involvement of fibrinogen in platelet aggregation and the release reaction. Sepharose 140-149 FA complementation group B Homo sapiens 36-39 1169259-1 1975 Monovalent goat antibody fragments (Fab) that were monospecific for human fibrinogen were isolated by affinity chromatography on fibrinogen-Sepharose and used as a direct probe for the involvement of fibrinogen in platelet aggregation and the release reaction. Sepharose 140-149 fibrinogen beta chain Homo sapiens 129-139 1169259-1 1975 Monovalent goat antibody fragments (Fab) that were monospecific for human fibrinogen were isolated by affinity chromatography on fibrinogen-Sepharose and used as a direct probe for the involvement of fibrinogen in platelet aggregation and the release reaction. Sepharose 140-149 fibrinogen beta chain Homo sapiens 129-139 1127225-1 1975 Properdin Factor B shows genetic polymorphism in the human and the polymorphic patterns in agarose gel electrophoresis suggest a tetrameric structure. Sepharose 91-98 complement factor B Homo sapiens 0-18 1150262-0 1975 Esterase D polymorphism: high-voltage agarose-gel electrophoresis and distribution of phenotypes in different European populations. Sepharose 38-45 esterase D Homo sapiens 0-10 1150262-1 1975 Esterase D phenotypes were determined in 1082 non-related individuals from the western region of Germany by agarose-gel electrophoresis. Sepharose 108-115 esterase D Homo sapiens 0-10 48477-0 1975 Affinity chromatography of human, rat and mouse alpha-fetoprotein on estradiol-sepharose adsorbents. Sepharose 79-88 alpha fetoprotein Mus musculus 48-65 1140197-6 1975 The latter ATP analogue, when bound to Sepharose through its terminal amino group, could be dephosphorylated to the corresponding ADP analogue with soluble hexokinase yielding glucose 6-phosphate in an enzymic "solidphase" fashion. Sepharose 39-48 hexokinase 1 Homo sapiens 156-166 53064-1 1975 A new rapid method for assaying total antithrombin activity has been developed based on the inactivation of thrombin incorporated into an agarose gel, during the radial diffusion of plasma in the gel. Sepharose 138-145 serpin family C member 1 Homo sapiens 38-50 53064-1 1975 A new rapid method for assaying total antithrombin activity has been developed based on the inactivation of thrombin incorporated into an agarose gel, during the radial diffusion of plasma in the gel. Sepharose 138-145 coagulation factor II, thrombin Homo sapiens 42-50 53064-2 1975 The area of thrombin inactivation is subsequently observed by the coagulation of fibrinogen in a separate agarose gel layer poured over the thrombin gel. Sepharose 106-113 coagulation factor II, thrombin Homo sapiens 12-20 53064-2 1975 The area of thrombin inactivation is subsequently observed by the coagulation of fibrinogen in a separate agarose gel layer poured over the thrombin gel. Sepharose 106-113 fibrinogen beta chain Homo sapiens 81-91 47266-4 1975 The presence of AFP in the serum was determined by double diffusion in agarose and by comparison also by quantitative radioimmunoassay. Sepharose 71-78 alpha-fetoprotein Rattus norvegicus 16-19 805207-2 1975 Agarose gel chromatography of normal plasma or purified fibrinogen previously incubated with small amounts of thrombin revealed the presence of a species of high molecular weight soluble fibrin complexes, which contained only small quantities of fibrinogen by immunological assays but which exhibited enhanced sensitivity to thrombin. Sepharose 0-7 coagulation factor II, thrombin Homo sapiens 110-118 805207-2 1975 Agarose gel chromatography of normal plasma or purified fibrinogen previously incubated with small amounts of thrombin revealed the presence of a species of high molecular weight soluble fibrin complexes, which contained only small quantities of fibrinogen by immunological assays but which exhibited enhanced sensitivity to thrombin. Sepharose 0-7 coagulation factor II, thrombin Homo sapiens 325-333 804516-5 1975 Fraction II was further separated into a minor and a major component (IIb) with Sepharose G-200. Sepharose 80-89 ATPase, class II, type 9B Mus musculus 70-73 164948-7 1975 After chromatography of crude extracts of asparaginyl-tRNA synthetase on Sepharose 4B the activity in the enzyme peak, which was of considerably larger molecular weight than any of the fractions found after purification could be enhanced by addition of the activator. Sepharose 73-82 LOW QUALITY PROTEIN: asparagine--tRNA ligase, cytoplasmic Oryctolagus cuniculus 42-69 1092337-0 1975 Kinetic studies on coenzyme binding and coenzyme dissociation in tryptophanase immobilized on sepharose. Sepharose 94-103 tryptophan 2,3-dioxygenase Homo sapiens 65-78 1131251-0 1975 Purification of cytochrome P-450 from bovin adrenocortical mitochondria by an "aniline-sepharose" and the properties. Sepharose 87-96 cytochrome P450 family 4 subfamily F member 3 Homo sapiens 16-32 50701-1 1975 Growth hormone conjugated to Sepharose-4 B was found to mimic the action of the free hormone in the stimulation of the uptake and incorporation of radioactive uridine into RNA in thymocytes in vitro. Sepharose 29-42 gonadotropin releasing hormone receptor Rattus norvegicus 0-14 165834-2 1975 Lipoamide dehydrogenase NADH: lipoamide oxidoreductase, (EC 1.6.4.3) from pig heart has been separated into two sets of isoenzymes by chromatography on lipoyl- and NAD+-derivatized Sepharose-4B matrices. Sepharose 181-190 dihydrolipoamide dehydrogenase Sus scrofa 0-23 810399-1 1975 Human fibrinogen was adsorbed on thrombin-activated fibrinogen which had been immobilized by covalent coupling with Sepharose-6B (Fibrin-Sepharose). Sepharose 116-125 fibrinogen beta chain Homo sapiens 6-16 810399-1 1975 Human fibrinogen was adsorbed on thrombin-activated fibrinogen which had been immobilized by covalent coupling with Sepharose-6B (Fibrin-Sepharose). Sepharose 116-125 coagulation factor II, thrombin Homo sapiens 33-41 810399-1 1975 Human fibrinogen was adsorbed on thrombin-activated fibrinogen which had been immobilized by covalent coupling with Sepharose-6B (Fibrin-Sepharose). Sepharose 116-125 fibrinogen beta chain Homo sapiens 52-62 1055365-0 1975 Structure of a soluble super-active insulin is revealed by the nature of the complex between cyanogen-bromide-activated sepharose and amines. Sepharose 120-129 insulin Homo sapiens 36-43 1055365-1 1975 Insulin-like material with elevated insulin specific acitivity is released from insulin-Sepharose in the presence of bovine-serum albumin. Sepharose 88-97 insulin Homo sapiens 0-7 1055365-1 1975 Insulin-like material with elevated insulin specific acitivity is released from insulin-Sepharose in the presence of bovine-serum albumin. Sepharose 88-97 insulin Homo sapiens 36-43 1055365-1 1975 Insulin-like material with elevated insulin specific acitivity is released from insulin-Sepharose in the presence of bovine-serum albumin. Sepharose 88-97 insulin Homo sapiens 80-87 1129299-0 1975 Differential binding to Sepharose-Con A of B12-binding proteins from human gastric juice. Sepharose 24-33 NADH:ubiquinone oxidoreductase subunit B3 Homo sapiens 43-46 234456-8 1975 The cross-linked complexes were obtained in homogeneous form by gel filtration on Sephadex and absorption of uncross-linked enzyme by affinity chromatography on alpha-lactalbumin-Sepharose in the presence of N-acetylglucosamine. Sepharose 179-188 lactalbumin alpha Homo sapiens 161-178 1191553-1 1975 Evidence has been presented that fibrin formation may be detected directly in agarose gels containing citrated plasma when they are treated with thrombin or calcium chloride. Sepharose 78-85 coagulation factor II, thrombin Homo sapiens 145-153 1168226-2 1975 Both sera contained material capable of precipitating C1q in agarose gel. Sepharose 61-68 complement C1q A chain Homo sapiens 54-57 1112786-2 1975 We purified the acetylcholinesterase 20-fold by differential centrifugation and filtration through a column of 4% agarose. Sepharose 114-121 acetylcholinesterase-like Aplysia californica 16-36 124728-3 1975 By two independent methods, ATPase activity measurements and analysis of elution patterns on agarose-ATP columns, it was shown that the introduction of two trinitrophenyl groups per myosin or one per heavy meromyosin subfragment 1 molecule is responsible for the remarkable change in the ATPase activities. Sepharose 93-100 myosin heavy chain 14 Homo sapiens 182-188 236187-2 1975 Various insoluble alpha-lactalbumins (bovine, bovine glyco-alpha-lactalbumin, human and human nitrated) have been prepared by coupling these proteins on to an agarose gel with use of cyanogen bromide. Sepharose 159-166 lactalbumin alpha Bos taurus 18-35 236187-6 1975 Some changes in the fluorescence spectrum (peak shifting towards longer wavelength) was observed for bovine alpha-lactalbumin and appeared to be due to alteration of the environment of the tryptophan side-chains in the protein upon coupling to the agarose gel. Sepharose 248-255 lactalbumin alpha Bos taurus 108-125 242317-1 1975 N-Acetyl-beta-hexosaminidase A was purified 1000-fold from human urine by chromatography on DEAE-Sephadex followed by concanavalin A--Sepharose affinity chromatography. Sepharose 134-143 O-GlcNAcase Homo sapiens 9-28 47318-2 1975 Human ceruloplasmin from fresh serum has been purified by chromatography on hydroxyapatite and Con A-Sepharose. Sepharose 101-110 ceruloplasmin Homo sapiens 6-19 235496-1 1975 An insoluble preparation of rat liver cathepsin D was obtained by coupling the enzyme to Enzacryl Polyacetal (EPA-cathepsin) and to CNBr-activated Sepharose 4B. Sepharose 147-156 cathepsin D Rattus norvegicus 38-49 235496-5 1975 Coupling of rat liver cathepsin D to activated Sepharose gave preparations active towards both protein and synthetic substrates. Sepharose 47-56 cathepsin D Rattus norvegicus 22-33 235496-9 1975 Proteolytic activity of the Sepharose-coupled cathepsin D was not inhibited by pepstatin, whereas that of the free enzyme was fully inhibited by this reagent. Sepharose 28-37 cathepsin D Rattus norvegicus 46-57 1099009-4 1975 Indirect MIF assays, using both capillary and agarose techniques, showed that migration inhibitory activity was found in thymus culture supernatants as well as in spleen culture supernatants of sensitized animals. Sepharose 46-53 macrophage migration inhibitory factor Rattus norvegicus 9-12 1132912-3 1975 The allergen binding was measured before and after absorption of the sera with anti-human IgE antibodies conjugated to Sepharose. Sepharose 119-128 immunoglobulin heavy constant epsilon Homo sapiens 90-93 803278-3 1975 The results of agarose-gel immunnodiffusion analyses indicated that although the antibody was specific for the acrosomal enzyme, it cross-related with ovine acrosin. Sepharose 15-22 acrosin Oryctolagus cuniculus 157-164 1095670-1 1975 Cellular immunity to hog intrinsic factor was detected by a modified agarose-leukocyte migration test in 18 patients with pernicious amemia. Sepharose 69-76 cobalamin binding intrinsic factor Homo sapiens 25-41 1054501-0 1975 Re-evaluation of Sepharose-insulin as a tool for the study of insulin action. Sepharose 17-26 insulin Homo sapiens 27-34 1054501-0 1975 Re-evaluation of Sepharose-insulin as a tool for the study of insulin action. Sepharose 17-26 insulin Homo sapiens 62-69 1054501-1 1975 The biological activity of Sepharose-insulin in different assays in vitro, e.g., stimulation of glucose oxidation, lipogenesis, and antilipolysis and activation of pyruvate dehydrogenase (EC 1.2.4.1) activity, has been investigated. Sepharose 27-36 insulin Homo sapiens 37-44 1054501-5 1975 After incubation with tissue or cells, Sepharose-insulin particles were separated by centrifugation from the medium. Sepharose 39-48 insulin Homo sapiens 49-56 1054501-7 1975 A quantitative evaluation of the soluble biological and immunological insulin activity in the supernatant accounted for the total insulin activity of Sepharose-insulin. Sepharose 150-159 insulin Homo sapiens 70-77 1054501-7 1975 A quantitative evaluation of the soluble biological and immunological insulin activity in the supernatant accounted for the total insulin activity of Sepharose-insulin. Sepharose 150-159 insulin Homo sapiens 130-137 812176-0 1975 Isolation of IgG3 from normal human sera and from a patient with multiple myeloma by using protein A-sepharose 4B. Sepharose 101-110 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 13-17 812176-1 1975 Affinity chromatography of IgG on protein A-Sepharose was used to isolate the human subclass IgG3 from normal serum and from a patient with multiple myeloma. Sepharose 44-53 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 93-97 4140510-8 1974 (f) DNase I-agarose affinity chromatography quantitatively retains purified skeletal muscle actin, and actin, specifically, from high-speed supernatants of whole cell extracts. Sepharose 12-19 deoxyribonuclease 1 Bos taurus 4-11 4140510-8 1974 (f) DNase I-agarose affinity chromatography quantitatively retains purified skeletal muscle actin, and actin, specifically, from high-speed supernatants of whole cell extracts. Sepharose 12-19 actin epsilon 1 Bos taurus 92-97 4140510-8 1974 (f) DNase I-agarose affinity chromatography quantitatively retains purified skeletal muscle actin, and actin, specifically, from high-speed supernatants of whole cell extracts. Sepharose 12-19 actin epsilon 1 Bos taurus 103-108 4140510-9 1974 (g) An antibody to purified inhibitor protein from calf thymus, used in indirect immunofluorescence on cells grown in culture, stains a two-dimensional network of fibers similar to that seen with an actin-specific antibody.The observation that actin can be isolated by DNase-agarose affinity chromatography provides a useful tool for the biochemical study of actin under different physiological conditions. Sepharose 275-282 actin epsilon 1 Bos taurus 244-249 4140510-9 1974 (g) An antibody to purified inhibitor protein from calf thymus, used in indirect immunofluorescence on cells grown in culture, stains a two-dimensional network of fibers similar to that seen with an actin-specific antibody.The observation that actin can be isolated by DNase-agarose affinity chromatography provides a useful tool for the biochemical study of actin under different physiological conditions. Sepharose 275-282 actin epsilon 1 Bos taurus 244-249 4213756-1 1974 Factor VIII in preparations from normal plasma is a large glycoprotein of greater than 2 million molecular weight which elutes in the exclusion volume of 4% agarose gels at an ionic strength of 0.15. Sepharose 157-164 coagulation factor VIII Bos taurus 0-11 4422493-0 1974 Affinity chromatography of human adipose tissue triacylglycerol lipase on agarose-linked dioleoylglycerol. Sepharose 74-81 lipase C, hepatic type Homo sapiens 48-70 4448165-1 1974 Interaction of leucine aminopeptidase with a substrate and an inhibitor bound to agarose and soluble dextran. Sepharose 81-88 carboxypeptidase Q Homo sapiens 23-37 4448170-0 1974 Cathepsin D: rapid isolation by affinity chromatography on haemoglobin-agarose resin. Sepharose 71-78 cathepsin D Homo sapiens 0-11 4461581-0 1974 Proceedings: Critical evaluation of sepharose-coupled insulin as a tool for studies of insulin action at the membrane level. Sepharose 36-45 insulin Homo sapiens 54-61 4461581-0 1974 Proceedings: Critical evaluation of sepharose-coupled insulin as a tool for studies of insulin action at the membrane level. Sepharose 36-45 insulin Homo sapiens 87-94 4473556-0 1974 Binding of carcinoembryonic antigen (CEA) to concanavalin A-sepharose: storage of high-specific-activity 125I-CEA. Sepharose 60-69 CEA cell adhesion molecule 3 Homo sapiens 11-35 4473556-0 1974 Binding of carcinoembryonic antigen (CEA) to concanavalin A-sepharose: storage of high-specific-activity 125I-CEA. Sepharose 60-69 CEA cell adhesion molecule 3 Homo sapiens 37-40 4375308-0 1974 [Determination of macrophage migration inhibitory factor in the HEPES-buffered agarose medium. Sepharose 79-86 macrophage migration inhibitory factor Homo sapiens 18-56 4477006-5 1974 Both alpha- and beta-trypsin may be isolated by chromatography of crude trypsin on the agarose derivative in an acetate buffer, pH4.0. Sepharose 87-94 serine protease 1 Bos taurus 5-28 4136230-6 1974 Rechromatographed p30, p21, p15, p11, and p10 had molecular weights of 27,000, 18,000, 15,000, 12,000, and 12,000 respectively, by co-electrophoresis with the marker proteins on sodium dodecyl sulfate polyacrylamide gel electrophoresis, clearly establishing that the latter two FeLV polypeptides comigrate to form one less band when compared to elution from agarose. Sepharose 358-365 centromere protein V Homo sapiens 18-21 4607118-3 1974 Dopamine-beta-hydroxylase is adsorbed to concanavalin A covalently bound to Sepharose and can be eluted with alpha-methylmannoside. Sepharose 76-85 dopamine beta-hydroxylase Homo sapiens 0-25 4216122-0 1974 Characterization of the venoms of various Bothrops species by immunoelectrophoresis and reaction with fibrinogen agarose. Sepharose 113-120 fibrinogen beta chain Homo sapiens 102-112 4136840-2 1974 Some of these substances are glycoproteins giving reactions of antigenic identity with carcinoembryonic antigen (CEA) derived from colorectal carcinoma and occur in aggregation with proteins showing antigen specificities of albumin, haptoglobin and the heavy chains of immunoglobulins G, A and M. Recoveries of CEA-like activities, following Sepharose 4B chromatography of urinary materials of molecular size > 3 x 10(4), varied from 40 to 1530%. Sepharose 342-354 CEA cell adhesion molecule 3 Homo sapiens 113-116 4855004-0 1974 The presence of zinc in carboxypeptidase C and coupling of the enzyme to sepharose and sephadex. Sepharose 73-82 cathepsin A Homo sapiens 24-42 4822532-0 1974 Erythroid colony formation in cultures of mouse and human bone marrow: analysis of the requirement for erythropoietin by gel filtration and affinity chromatography on agarose-concanavalin A. Sepharose 167-174 erythropoietin Homo sapiens 103-117 4603595-0 1974 [Identification of hereditary types of alpha-1-antitrypsin by acrylamide-agarose gel electrophoresis at pH 4.8]. Sepharose 73-80 serpin family A member 1 Homo sapiens 39-58 4856266-0 1974 The interaction of lysine vasopressin with free and agarose-bound bovine neurophysin II. Sepharose 52-59 arginine vasopressin Bos taurus 73-87 4275975-0 1974 On the reactivity of human leukocytes to PPD in Clausen"s agarose migration technique. Sepharose 58-65 cellular communication network factor 6 Homo sapiens 41-44 4450303-0 1974 A simple 2-step isolation procedure for human antithrombin II/III and its biological activity after insolubilization to agarose. Sepharose 120-127 serpin family C member 1 Homo sapiens 46-65 4450806-0 1974 [Investigations on the polymorphism of red cell esterase D by high voltage agarose gel electrophoresis (author"s transl)]. Sepharose 75-82 esterase D Homo sapiens 48-58 4205557-0 1973 Active subunits of transaldolase bound to sepharose. Sepharose 42-51 transaldolase 1 Homo sapiens 19-32 4542944-7 1973 This finding, in addition to the observation that agarose gel chromatography fractions that have VIII(AHF) procoagulant activity also have VIII(VWF) activity, strongly suggests that the von Willebrand factor is associated with the factor VIII molecule. Sepharose 50-57 cytochrome c oxidase subunit 8A Homo sapiens 97-101 4542944-7 1973 This finding, in addition to the observation that agarose gel chromatography fractions that have VIII(AHF) procoagulant activity also have VIII(VWF) activity, strongly suggests that the von Willebrand factor is associated with the factor VIII molecule. Sepharose 50-57 von Willebrand factor Homo sapiens 186-207 4517946-1 1973 Nerve growth factor was insolubilized by covalent attachment to Sepharose beads. Sepharose 64-73 nerve growth factor Homo sapiens 0-19 4517946-2 1973 Nerve growth factor-Sepharose was biologically active in both the neurite outgrowth assay for nerve growth factor and in preserving responsive neurons in vitro. Sepharose 20-29 nerve growth factor Homo sapiens 0-19 4517946-2 1973 Nerve growth factor-Sepharose was biologically active in both the neurite outgrowth assay for nerve growth factor and in preserving responsive neurons in vitro. Sepharose 20-29 nerve growth factor Homo sapiens 94-113 4517946-3 1973 Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. Sepharose 156-165 nerve growth factor Homo sapiens 63-82 4517946-3 1973 Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. Sepharose 156-165 nerve growth factor Homo sapiens 136-155 4517946-3 1973 Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. Sepharose 156-165 nerve growth factor Homo sapiens 136-155 4517946-3 1973 Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. Sepharose 156-165 nerve growth factor Homo sapiens 136-155 4517946-3 1973 Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. Sepharose 200-209 nerve growth factor Homo sapiens 63-82 4517946-3 1973 Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. Sepharose 200-209 nerve growth factor Homo sapiens 136-155 4517946-3 1973 Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. Sepharose 200-209 nerve growth factor Homo sapiens 136-155 4517946-3 1973 Modification of the bioassay to detect solubilized activity of nerve growth factor and histological examination of ganglia treated with nerve growth factor-Sepharose revealed that nerve growth factor-Sepharose prepared by reaction in 6 M guanidine hydrochloride released negligible amounts of solubilized nerve growth factor activity. Sepharose 200-209 nerve growth factor Homo sapiens 136-155 4199413-2 1973 Normal human antihemophilic factor (AHF, factor VIII) and the protein antigenically related to it in hemophilic plasma both appeared in the void volume of columns of agarose (Sepharose 4B) during purification of these agents. Sepharose 166-173 coagulation factor VIII Homo sapiens 13-34 4199413-2 1973 Normal human antihemophilic factor (AHF, factor VIII) and the protein antigenically related to it in hemophilic plasma both appeared in the void volume of columns of agarose (Sepharose 4B) during purification of these agents. Sepharose 166-173 coagulation factor VIII Homo sapiens 36-39 4199413-2 1973 Normal human antihemophilic factor (AHF, factor VIII) and the protein antigenically related to it in hemophilic plasma both appeared in the void volume of columns of agarose (Sepharose 4B) during purification of these agents. Sepharose 175-187 coagulation factor VIII Homo sapiens 13-34 4199413-2 1973 Normal human antihemophilic factor (AHF, factor VIII) and the protein antigenically related to it in hemophilic plasma both appeared in the void volume of columns of agarose (Sepharose 4B) during purification of these agents. Sepharose 175-187 coagulation factor VIII Homo sapiens 36-39 19999708-0 1973 Alpha1-fetoprotein: separation of two molecular variants by affinity chromatography with concanavalin A-agarose. Sepharose 104-111 alpha fetoprotein Homo sapiens 0-18 19999708-1 1973 Alpha1-Fetoprotein present in fetal/newborn rat serum and in hepatoma-bearing human serum has been resolved into two molecular variants by concanavalin A-agarose affinity chromatography. Sepharose 154-161 alpha fetoprotein Homo sapiens 0-18 19999741-0 1973 Purification of 125I-labelled lysine-vasopressin by affinity chromatography on sepharose-bound neurophysins. Sepharose 79-88 arginine vasopressin Homo sapiens 37-48 19999741-1 1973 Purification of 125I-labelled lysine-vasopressin has been achieved by affinity chromatography on a Sepharose 4 B conjugate of porcine neurophysins. Sepharose 99-108 arginine vasopressin Homo sapiens 37-48 4525167-1 1973 Factor VIII is a large protein molecule of molecular weight 2,000,000 or larger that elutes in the void volume on agarose gel chromatography. Sepharose 114-121 coagulation factor VIII Canis lupus familiaris 0-11 4525167-9 1973 Finally, when the large carrier protein and the small active fragment of factor VIII were separated by gel chromatography, mixed, and dialyzed free of Ca(2+), they recombined to form a large active molecule that appeared in the void volume on agarose gel chromatography. Sepharose 243-250 coagulation factor VIII Canis lupus familiaris 73-84 4199020-0 1973 [Presence in the genus Bacillus of systems which hydrolyse the beta(1-4) bonds of agarose]. Sepharose 82-89 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 63-71 4736323-0 1973 Affinity chromatography: specific binding of hemoglobin on agarose linked haptoglobin. Sepharose 59-66 haptoglobin Homo sapiens 74-85 4542589-0 1973 Purification of human alpha 1-antitrypsin by affinity chromatography on sepharose bound concanavalin A. Sepharose 72-81 serpin family A member 1 Homo sapiens 22-41 4575958-0 1973 Preparation of pyridoxal 5"-phosphate-bound sepharose and its use for immobilization of tryptophanase. Sepharose 44-53 tryptophan 2,3-dioxygenase Homo sapiens 88-101 4198858-0 1973 [Separation of 2 forms of endobronchial alpha 1-antitrypsin using affinity chromatography on "Sepharose-trypsin"]. Sepharose 94-103 serpin family A member 1 Homo sapiens 40-59 4721620-10 1973 The 5"-nucleotidase was purified further by gel filtration on Sepharose and Sephadex columns equilibrated with sarcosyl-Tris buffer, to give a single glycoprotein band on sodium dodecyl sulphate-polyacrylamide-gel electrophoresis. Sepharose 62-71 5' nucleotidase, ecto Mus musculus 4-19 4689220-0 1973 Biological activity of insulin-sepharose? Sepharose 31-40 insulin Homo sapiens 23-30 4351804-1 1973 Immobilized insulin, prepared by coupling insulin directly to agarose or through hydrocarbon "connecting arms," was demonstrated to be capable of firmly binding intact adipocytes and their ghosts. Sepharose 62-69 insulin Homo sapiens 12-19 4351804-2 1973 Various lines of evidence indicate that the insulin receptor on the plasma membrane, in addition to the insulin coupled to the agarose, was responsible for the observed binding. Sepharose 127-134 insulin receptor Homo sapiens 44-60 4351804-2 1973 Various lines of evidence indicate that the insulin receptor on the plasma membrane, in addition to the insulin coupled to the agarose, was responsible for the observed binding. Sepharose 127-134 insulin Homo sapiens 44-51 4351804-3 1973 This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. Sepharose 117-126 insulin Homo sapiens 109-116 4351804-3 1973 This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. Sepharose 117-126 insulin Homo sapiens 201-208 4351804-3 1973 This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. Sepharose 117-126 insulin Homo sapiens 201-208 4351804-3 1973 This evidence includes: (a) the finding that increasing the "arm" length increased the binding capacities of insulin-Sepharose affinity chromatographic columns, (b) specific inhibition and reversal by insulin and antiserum to insulin of the binding, as compared to lesser effects by other peptide hormones, (c) the indication that only the plasma membrane sacs, not the other cellular contaminants in the crude ghosts, are capable of binding, and (d) the impairment and restoration of trypsin-sensitive membrane binding sites that are also required for insulin biosensitivity. Sepharose 117-126 insulin Homo sapiens 201-208 4351804-5 1973 By use of the differential buoyant densities of the various cell-bead complexes that resulted from the interaction of adipocytes with insulin-Sepharose, a new procedure was developed to demonstrate and study the binding. Sepharose 142-151 insulin Homo sapiens 134-141 4630182-0 1973 Growth hormone covalently bound to Sepharose. Sepharose 35-44 growth hormone 1 Homo sapiens 0-14 4682860-0 1973 Growth hormone covalently bound to Sepharose. Sepharose 35-44 growth hormone 1 Homo sapiens 0-14 4682860-2 1973 Study of the biological activity of a growth hormone-Sepharose complex in adipose tissue and diaphragm muscle. Sepharose 53-62 growth hormone 1 Homo sapiens 38-52 4684036-0 1973 Migration inhibitory effect of cell-free supernatants from tuberculin-stimulated cultures of human mononuclear leukocytes demonstrated by two-step MIF agarose assay. Sepharose 151-158 macrophage migration inhibitory factor Homo sapiens 147-150 4633808-0 1973 Validation of a "clearing" assay for milk lipoprotein lipase in agarose gel. Sepharose 64-71 lipoprotein lipase Bos taurus 42-60 4633808-1 1973 We have developed a simplified method for the quantitative assay of lipoprotein lipase in cow"s milk based on the hydrolysis of a glyceride emulsion in semisolid agarose gel. Sepharose 162-169 lipoprotein lipase Bos taurus 68-86 4216114-0 1973 [Identification and preparation of high-molecular derivatives of fibrinogen from human plasma by means of PAA-gel electrophoresis and agarose-gel chromatography]. Sepharose 134-141 fibrinogen beta chain Homo sapiens 65-75 4118356-10 1972 The in vitro production of partially desialylated TBG from T(4)-Sepharose which had been previously exposed to large volumes of serum may be due to adsorption of neuraminidases to the Sepharose either directly from serum or as the result of bacterial contamination. Sepharose 64-73 serpin family A member 7 Homo sapiens 50-53 4636728-0 1972 Tritium labelling of 8-lysine vasopressin and its purification by affinity chromatography on sepharose bound neurophysins. Sepharose 93-102 arginine vasopressin Homo sapiens 30-41 5083616-0 1972 Purification and fractionation of acetylcholinesterase into subspecies by affinity chromatography on a d-tubocurarine--sepharose column. Sepharose 119-128 acetylcholinesterase (Cartwright blood group) Homo sapiens 34-54 11946642-0 1972 Isolation of a trypsin inhibitor (Kunitz inhibitor) from bovine ovary by affinity chromatography through trypsin-sepharose. Sepharose 113-122 trophoblast Kunitz domain protein 1 Bos taurus 15-50 4626192-1 1972 Leucine aminopeptidase bound to Sepharose and Sephadex beads. Sepharose 32-41 carboxypeptidase Q Homo sapiens 8-22 4346743-10 1972 The binding of both glyceraldehyde 3-phosphate dehydrogenase and lactate dehydrogenase to the gel tested, AMP-Sepharose, is strong enough to resist elution by gradients of KCl of up to at least 0.5m. Sepharose 110-119 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 20-60 4504339-3 1972 Several insulin-agarose derivatives have been synthesized that can efficiently extract the insulin-binding protein from the detergent extracts of the membranes. Sepharose 16-23 insulin Homo sapiens 8-15 4504339-3 1972 Several insulin-agarose derivatives have been synthesized that can efficiently extract the insulin-binding protein from the detergent extracts of the membranes. Sepharose 16-23 insulin Homo sapiens 91-98 5049611-0 1972 Separation of sub-units of antihemophilic factor (AHF) by agarose gel chromatography. Sepharose 58-65 coagulation factor VIII Homo sapiens 27-48 5049611-0 1972 Separation of sub-units of antihemophilic factor (AHF) by agarose gel chromatography. Sepharose 58-65 coagulation factor VIII Homo sapiens 50-53 5019971-0 1972 Anomalous behaviour of catalase on agarose gel. Sepharose 35-42 catalase Homo sapiens 23-31 5059200-1 1972 When NH(4)OH-NH(4)Cl extracts of adipose acetone powder were applied to agarose gel chromatography columns, two peaks of lipoprotein lipase were eluted. Sepharose 72-79 lipoprotein lipase Homo sapiens 121-139 5288238-1 1971 Sodium dodecyl sulfate-acrylamide gel electrophoresis and molecular-sieve chromatography on 8% agarose demonstrate the existence of a very high molecular weight (500,000-600,000), proline-rich protein in cultured 3T6 fibroblasts that appears to be the precursor molecule (procollagen) of collagen. Sepharose 95-102 complement component 4 binding protein alpha Homo sapiens 180-200 5570426-1 1971 Stimulation of glycogen synthetase in an in vitro liver system by insulin bound to sepharose. Sepharose 83-92 insulin Homo sapiens 66-73 4327488-0 1971 ACTH diazotized to agarose: effects on isolated adrenal cells. Sepharose 19-26 proopiomelanocortin Homo sapiens 0-4 4100685-6 1971 The electrophoretic mobility of purified C1r is that of a beta-globulin on disc acrylamide electrophoresis and on agarose electrophoresis at pH 8.6. Sepharose 114-121 complement C1r subcomponent Oryctolagus cuniculus 41-44 4258201-0 1971 The identification of fibrinogen derivatives in plasma and serum by agarose gel filtration. Sepharose 68-75 fibrinogen beta chain Homo sapiens 22-32 5483637-0 1970 Stimulation of RNA synthesis in isolated mammary cells by insulin and prolactin bound to sepharose. Sepharose 89-98 insulin Homo sapiens 58-65 5483637-0 1970 Stimulation of RNA synthesis in isolated mammary cells by insulin and prolactin bound to sepharose. Sepharose 89-98 prolactin Homo sapiens 70-79 5493858-4 1970 Propionic acid eluted 60-100% of the antibody adsorbed by Sepharose-myoglobin, and 60-90% of the eluted protein was precipitable antibody. Sepharose 58-67 myoglobin Physeter catodon 68-77 4250395-0 1970 Molecular sieve chromatography of myosin on agarose columns. Sepharose 44-51 myosin heavy chain 14 Homo sapiens 34-40 4987004-0 1970 [Composition of human alpha 2-macroglobulin isolated by filtration on agarose gel]. Sepharose 70-77 alpha-2-macroglobulin Homo sapiens 22-43 5437344-0 1970 Immunological reactivity of insulin to sepharose coupled with insulin-antibody---its use for the extraction of insulin from serum. Sepharose 39-48 insulin Homo sapiens 28-35 4190971-0 1970 Reactions in agarose gel between C1q and aggregated gamma globulin. Sepharose 13-20 complement C1q A chain Homo sapiens 33-36 4244455-5 1970 Agarose and acrylamide gel immunoelectrophoresis of a plasmin, inhibitor mixture showed the appearance of an additional precipitin band with immunologic reactivity similar to that of the untreated inhibitor. Sepharose 0-7 plasminogen Homo sapiens 54-61 4907878-4 1969 Agarose-chymotrypsin was stable over the range pH2-9, but agarose-rennin released active enzyme into solution at above pH2 and aminoethylcellulose-rennin was similarly unstable at certain pH values. Sepharose 0-7 polyhomeotic homolog 2 Homo sapiens 47-50 4907878-4 1969 Agarose-chymotrypsin was stable over the range pH2-9, but agarose-rennin released active enzyme into solution at above pH2 and aminoethylcellulose-rennin was similarly unstable at certain pH values. Sepharose 58-65 polyhomeotic homolog 2 Homo sapiens 119-122 5257136-1 1969 Insulin can be covalently attached to a large polymers of Sepharose through the alpha-amino group of the N-terminal residue of the B chain, or through the epsilon-amino group of its lysyl residue. Sepharose 58-67 insulin Homo sapiens 0-7 5257136-3 1969 The effects occur with concentrations of insulin-Sepharose that are nearly as low as those of native insulin, and the maximal responses are the same. Sepharose 49-58 insulin Homo sapiens 41-48 5815208-0 1969 Insulin-sepharose: immunoreactivity and use in the purification of antibody. Sepharose 8-17 insulin Homo sapiens 0-7 6038133-0 1967 Determination of transferrin by Laurell electrophoresis in antibody containing agarose gel. Sepharose 79-86 transferrin Homo sapiens 17-28 33250420-3 2021 The carrier PEN has been demonstrated to possess favorable ability to condense miR-29a into stable nanoparticles and protect miR-29a against the nuclease degradation, using agarose gel retardation assay. Sepharose 173-180 proprotein convertase subtilisin/kexin type 1 inhibitor Homo sapiens 12-15 182529-1 1976 At concentrations of 200 muM NADH and 0.5 M NaCl LDH-X is separated from the other LDH isozymes of mouse testes on oxamate-sepharose. Sepharose 123-132 lactate dehydrogenase C Mus musculus 49-54 182529-1 1976 At concentrations of 200 muM NADH and 0.5 M NaCl LDH-X is separated from the other LDH isozymes of mouse testes on oxamate-sepharose. Sepharose 123-132 lactate dehydrogenase C Mus musculus 49-52 34057448-6 2021 However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcepsilonRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite. Sepharose 35-42 KIT proto-oncogene receptor tyrosine kinase Mus musculus 194-197 34057448-6 2021 However, BMMCs cultured on the CNC/agarose/D-mannitol substrate appeared to slightly increase their expression of the high-affinity IgE receptor (FcepsilonRI) and the stem cell factor receptor (Kit; CD117), although this does not appear to be dependent on the amount of CNC in the bioink composite. Sepharose 35-42 KIT proto-oncogene receptor tyrosine kinase Mus musculus 199-204 33844311-11 2021 As the measurement with agarose embedded electrolyte allowed non-invasive identification of the Cldn1 knockdown, this opens the way to detailedin vivo skin barrier assessment. Sepharose 24-31 claudin 1 Homo sapiens 96-101 33595270-5 2021 So, an optimized formation of the Li-O coordinate bond (gLi-O(r) = 8.78) and improved diffusion coefficient of Li+ (DLi+) (71 x 10-6 cm2 s-1) were obtained in the agarose/PVA model. Sepharose 163-170 GLI family zinc finger 1 Homo sapiens 56-59 33732900-4 2021 Methods: Neuraminidase was immobilized onto agarose activated with cyanate ester groups and further used for desialylation of model glycoproteins, a lysate from tumour cells and tumour cells. Sepharose 44-51 neuraminidase 1 Bos taurus 9-22 33672694-2 2021 The ability of human topoisomerase I (hTOPI) and II to relax supercoiled plasmid DNA in the presence of various concentrations of the tacrine-coumarin hybrid molecules was studied with agarose gel electrophoresis. Sepharose 185-192 DNA topoisomerase I Homo sapiens 38-43 33035617-5 2021 PCR-RFLP and agarose gel electrophoresis technology were used to type the SNP site of MOGAT1 gene at g.25940T > C in this study. Sepharose 13-20 monoacylglycerol O-acyltransferase 1 Bos taurus 86-92 33583374-3 2021 METHODS: The smart pH sensitive HP-beta-CD/agarose-g-poly(MAA) hydrogel network was developed using an aqueous free radical polymerization technique. Sepharose 43-50 ACD shelterin complex subunit and telomerase recruitment factor Homo sapiens 35-42 958454-0 1976 Combination of insulin chains on an anti-insulin antibody-Sepharose column. Sepharose 58-67 insulin Homo sapiens 15-22 33955691-4 2021 The agarose gel to confirm the deletion in the Rag1 gene in Rag1 knocked-out blastocyst. Sepharose 4-11 recombination activating 1 Mus musculus 47-51 33955691-4 2021 The agarose gel to confirm the deletion in the Rag1 gene in Rag1 knocked-out blastocyst. Sepharose 4-11 recombination activating 1 Mus musculus 60-64 33439347-2 2021 In this study, a mammosphere model has been characterised using human mammary epithelial cells grown on either mouse extracellular matrix or agarose and showed insulin is essential for formation of mammospheres. Sepharose 141-148 insulin Homo sapiens 160-167 33586042-8 2021 ALB-Cys34SNO was prepared by reacting n-butylnitrite with albumin after selective extraction from plasma of a healthy donor on HiTrapBlue Sepharose cartridges. Sepharose 138-147 albumin Homo sapiens 0-3 33586042-9 2021 ALB-Cys34SNO was used in platelet aggregation measurements after extended purification on HiTrapBlue Sepharose and enrichment by ultrafiltration (cutoff, 20 kDa). Sepharose 101-110 albumin Homo sapiens 0-3 33207020-6 2021 For soft tissues, agarose gels with varying concentrations of agarose, gadolinium (Gd) and sodium fluoride (NaF) were developed. Sepharose 18-25 C-X-C motif chemokine ligand 8 Homo sapiens 108-111 33540369-9 2021 The optimized number of pGCs (5 x 104 cells/well) in a 3D agarose matrix led to a significantly higher blastocyst formation, increased BMP15 gene and protein expression, and intra-oocyte ATP levels; moreover, it induced significantly lower intra-oocyte ROS contents, pro-apoptotic BAX gene expression, and apoptotic index, compared to control. Sepharose 58-65 bone morphogenetic protein 15 Sus scrofa 135-140 33540369-9 2021 The optimized number of pGCs (5 x 104 cells/well) in a 3D agarose matrix led to a significantly higher blastocyst formation, increased BMP15 gene and protein expression, and intra-oocyte ATP levels; moreover, it induced significantly lower intra-oocyte ROS contents, pro-apoptotic BAX gene expression, and apoptotic index, compared to control. Sepharose 58-65 apoptosis regulator BAX Sus scrofa 281-284 33386500-7 2021 RESULTS: The recombinant DNA constructs were confirmed as the ~ 867 bp and ~ 648 bp bands related tonef-vpr-gp160-p24 andnef genes on agarose gel. Sepharose 134-141 glutamyl aminopeptidase Homo sapiens 108-113 33386500-7 2021 RESULTS: The recombinant DNA constructs were confirmed as the ~ 867 bp and ~ 648 bp bands related tonef-vpr-gp160-p24 andnef genes on agarose gel. Sepharose 134-141 transmembrane p24 trafficking protein 2 Homo sapiens 114-117 33454336-5 2021 Native agarose gel analysis showed changes in mobility of human transferrin upon Fe3+ binding. Sepharose 7-14 transferrin Homo sapiens 64-75 33454336-8 2021 We have extracted bands of bovine serum albumin from the agarose native gel for sodium dodecylsulfate gel electrophoresis analysis, showing degradation of aged sample. Sepharose 57-64 albumin Homo sapiens 34-47 33464542-8 2021 The recombinant lentivirus vector IL-1Ra was successfully constructed by 2% agarose gel electrophoresis. Sepharose 76-83 interleukin 1 receptor antagonist Homo sapiens 34-40 33732811-8 2021 The knockdown efficiency is measured by RNA isolation and semiquantitative RT-PCR reaction followed by agarose gel electrophoresis. Sepharose 103-110 knockdown Drosophila melanogaster 4-13 33681597-9 2021 Agarose gel electrophoresis (AGE) and dynamic light scattering (DLS) showed that the binding ratio was roughly 2:1 (HSA/QDs), resembling sandwich-like structures. Sepharose 0-7 renin binding protein Homo sapiens 29-32 33524049-8 2021 In addition, we showed that an imbalance in binding of HSP47-R222S to unfolded type I collagen chains in a gelatin sepharose pulldown assay results in increased binding of other chaperones and modifying enzymes. Sepharose 115-124 serpin family H member 1 Homo sapiens 55-60 33514813-3 2021 Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. Sepharose 81-88 ribonuclease A family member 1, pancreatic Homo sapiens 0-14 33514813-3 2021 Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. Sepharose 81-88 ribonuclease A family member 1, pancreatic Homo sapiens 16-23 33514813-3 2021 Ribonuclease A (RNase A) was used to investigate a number of different activated-agarose carriers: cyanogen bromide (CNBr)-activated-agarose and glyoxyl-agarose showed to preserve the appropriate orientation of the protein for receptor binding. Sepharose 133-140 ribonuclease A family member 1, pancreatic Homo sapiens 0-14 33514813-7 2021 Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF. Sepharose 117-124 epidermal growth factor Homo sapiens 125-128 33514813-7 2021 Although both of the immobilized samples show dose-dependency when seeded with high number of fibroblast cells, CNBr-agarose-EGF showed a significantly high activity at 100 ng/mL and 72 h incubation, compared to glyoxyl-agarose-EGF. Sepharose 117-124 epidermal growth factor Homo sapiens 228-231 33508088-9 2021 Incubation of recombinant GST-YY1B and GST-YY1Bm with SMC lysates followed by precipitation with glutathione-agarose beads and mass spectrometric analysis identified a novel interaction between YY1B and BASP1. Sepharose 109-116 YY1 transcription factor Rattus norvegicus 30-34 33038594-5 2021 Here, we designed an agarose hydrogel that releases Fas ligand (FasL), a protein that can induce apoptosis of cytotoxic CD8+ T cells. Sepharose 21-28 Fas ligand Homo sapiens 52-62 33038594-5 2021 Here, we designed an agarose hydrogel that releases Fas ligand (FasL), a protein that can induce apoptosis of cytotoxic CD8+ T cells. Sepharose 21-28 Fas ligand Homo sapiens 64-68 33038594-7 2021 Agarose-FasL hydrogels locally reduced the host cytotoxic CD8+ T cell population and enhanced the survival of allogeneic MSCs transplanted near the injury site. Sepharose 0-7 Fas ligand Homo sapiens 8-12 32955069-5 2020 Interestingly, a convenient, affordable, and instrument-free portable test kit was fabricated to visually monitor CEA via rooting the aptamer/MoS2 NS system into an agarose hydrogel. Sepharose 165-172 CEA cell adhesion molecule 3 Homo sapiens 114-117 33840686-8 2021 IL-12p40 and IL-6 synthesis by BM-DCs was completely diminished upon stimulation with HA treated with concanavalin A (ConA)-bound sepharose beads. Sepharose 130-139 interleukin 6 Mus musculus 13-17 33456581-5 2021 Regulation of the HSP system was investigated by immunoprecipitation, drug affinity responsive target stability assay, binding experiments using ATP-agarose beads and biotinylated drug, and docking analysis. Sepharose 149-156 heat shock protein 90 beta family member 2, pseudogene Homo sapiens 18-21 33367644-3 2020 This immunoprecipitation method relies on extraction of butyrylcholinesterase (BChE), a biomarker of OP poisoning that adducts OP compounds, from human serum using agarose beads conjugated to anti-BChE antibodies. Sepharose 164-171 butyrylcholinesterase Homo sapiens 56-77 33367644-3 2020 This immunoprecipitation method relies on extraction of butyrylcholinesterase (BChE), a biomarker of OP poisoning that adducts OP compounds, from human serum using agarose beads conjugated to anti-BChE antibodies. Sepharose 164-171 butyrylcholinesterase Homo sapiens 79-83 33125342-3 2021 A Src family tyrosine kinase with an apparent molecular mass of about 62 kDa was purified to homogeneity from the soluble fraction of dark-adapted bovine retinas after three consecutive purification steps: omega-aminooctyl-agarose hydrophobic chromatography, Cibacron blue 3GA-agarose pseudo-affinity chromatography, and alpha-casein-agarose affinity chromatography. Sepharose 223-230 SRC proto-oncogene, non-receptor tyrosine kinase Bos taurus 2-5 33136246-8 2020 The MMP-3 promoter methylation status was analayzed with MSP and determined with agarose gel electrophoresis. Sepharose 81-88 matrix metallopeptidase 3 Homo sapiens 4-9 32768724-6 2020 In vitro maturation (IVM) of pig COCs using a 1% (w/v) agarose matrix resulted in significantly higher blastocyst formation, cumulus expansion, gene expression of BMP15, HIF1A and VEGFA, protein expression of BMP15, and intraoocyte ATP levels, and there was significantly reduced expression of a pro-apoptotic gene and ACTN4 gene and a reduction in TUNEL indices. Sepharose 55-62 bone morphogenetic protein 15 Sus scrofa 163-168 32768724-6 2020 In vitro maturation (IVM) of pig COCs using a 1% (w/v) agarose matrix resulted in significantly higher blastocyst formation, cumulus expansion, gene expression of BMP15, HIF1A and VEGFA, protein expression of BMP15, and intraoocyte ATP levels, and there was significantly reduced expression of a pro-apoptotic gene and ACTN4 gene and a reduction in TUNEL indices. Sepharose 55-62 hypoxia inducible factor 1 subunit alpha Sus scrofa 170-175 32768724-6 2020 In vitro maturation (IVM) of pig COCs using a 1% (w/v) agarose matrix resulted in significantly higher blastocyst formation, cumulus expansion, gene expression of BMP15, HIF1A and VEGFA, protein expression of BMP15, and intraoocyte ATP levels, and there was significantly reduced expression of a pro-apoptotic gene and ACTN4 gene and a reduction in TUNEL indices. Sepharose 55-62 vascular endothelial growth factor A Sus scrofa 180-185 32768724-6 2020 In vitro maturation (IVM) of pig COCs using a 1% (w/v) agarose matrix resulted in significantly higher blastocyst formation, cumulus expansion, gene expression of BMP15, HIF1A and VEGFA, protein expression of BMP15, and intraoocyte ATP levels, and there was significantly reduced expression of a pro-apoptotic gene and ACTN4 gene and a reduction in TUNEL indices. Sepharose 55-62 bone morphogenetic protein 15 Sus scrofa 209-214 32768724-6 2020 In vitro maturation (IVM) of pig COCs using a 1% (w/v) agarose matrix resulted in significantly higher blastocyst formation, cumulus expansion, gene expression of BMP15, HIF1A and VEGFA, protein expression of BMP15, and intraoocyte ATP levels, and there was significantly reduced expression of a pro-apoptotic gene and ACTN4 gene and a reduction in TUNEL indices. Sepharose 55-62 actinin alpha 4 Sus scrofa 319-324 32434111-5 2020 Of the materials studied, an anthocyanin-agarose film is nominated as the optimum materials with the best colorimetric performance. Sepharose 41-48 DDB1 and CUL4 associated factor 7 Homo sapiens 29-40 33369604-5 2020 Splicing activity in the U1-depleted extract can be reconstituted by the galectin-3 - U1 snRNP particle trapped on agarose beads covalently coupled with anti-galectin-3 antibodies. Sepharose 115-122 galectin 3 Homo sapiens 73-83 33369604-5 2020 Splicing activity in the U1-depleted extract can be reconstituted by the galectin-3 - U1 snRNP particle trapped on agarose beads covalently coupled with anti-galectin-3 antibodies. Sepharose 115-122 RNA, U1 small nuclear 1 Homo sapiens 86-94 33369604-5 2020 Splicing activity in the U1-depleted extract can be reconstituted by the galectin-3 - U1 snRNP particle trapped on agarose beads covalently coupled with anti-galectin-3 antibodies. Sepharose 115-122 galectin 3 Homo sapiens 158-168 33200936-1 2020 A high-throughput agarose gel electrophoresis (AGE) analytical method has been developed to separate lignin fractions according to their molecular weight (Mw), charge, and shape. Sepharose 18-25 renin binding protein Homo sapiens 47-50 32990813-2 2020 The examination of heme-binding proteins in colostrum and milk using hemin-agarose beads (HA) showed alpha-casein, kappa-casein and lactoferrin (Lf) to be heme-binding proteins. Sepharose 75-82 HEME Bos taurus 19-23 33196158-5 2020 Hydrophobic oil microparticles are produced by the simple plasma treatment of the DM, and agarose microparticles encapsulating bovine serum albumin (in a well-dispersed state) are produced by submerging the DM in fluorinated oil. Sepharose 90-97 albumin Homo sapiens 134-147 32669211-6 2020 MATERIALS AND METHODS: Recombinant QnrB19 was expressed in E. coli and purified by Ni-NTA agarose column chromatography. Sepharose 90-97 QnrB19 Escherichia coli 35-41 32690883-5 2020 We show that the Herceptin (the brand name of trastuzumab, a monoclonal antibody drug used to treat breast cancer) and the much smaller cytokine interferon alpha-2a can be purified by site-specifically tagging them with adamantylammonium using the enzyme sortase A, followed by high-affinity binding with CB[7]-conjugated agarose beads and the recovery of the protein using a guest with a stronger affinity for CB[7]. Sepharose 322-329 interferon alpha 2 Homo sapiens 145-164 33065540-10 2020 Using magnetic sepharose ZP2-bound beads might be a valuable tool to improve the fertilisation rate in pigs. Sepharose 15-24 zona pellucida glycoprotein 2 Sus scrofa 25-28 33178677-2 2020 In this work, a food safety immobilization system for L-asparaginase (L-ASNase) consisting of food-grade agarose (Aga) spheres and N-hydroxysuccinimide esters was developed to decrease the formation of acrylamide in a fluid food model system. Sepharose 105-112 asparaginase and isoaspartyl peptidase 1 Homo sapiens 54-68 33178677-2 2020 In this work, a food safety immobilization system for L-asparaginase (L-ASNase) consisting of food-grade agarose (Aga) spheres and N-hydroxysuccinimide esters was developed to decrease the formation of acrylamide in a fluid food model system. Sepharose 105-112 asparaginase and isoaspartyl peptidase 1 Homo sapiens 70-78 33178677-2 2020 In this work, a food safety immobilization system for L-asparaginase (L-ASNase) consisting of food-grade agarose (Aga) spheres and N-hydroxysuccinimide esters was developed to decrease the formation of acrylamide in a fluid food model system. Sepharose 114-117 asparaginase and isoaspartyl peptidase 1 Homo sapiens 54-68 33178677-2 2020 In this work, a food safety immobilization system for L-asparaginase (L-ASNase) consisting of food-grade agarose (Aga) spheres and N-hydroxysuccinimide esters was developed to decrease the formation of acrylamide in a fluid food model system. Sepharose 114-117 asparaginase and isoaspartyl peptidase 1 Homo sapiens 70-78 33028117-4 2021 The CS content (microg/mg dry cartilage) after proteolysis was determined by densitometry, using agarose-gel electrophoresis. Sepharose 97-104 citrate synthase Homo sapiens 4-6 32623922-13 2020 We introduce the first VEGF-based highly efficient sFlt-1 apheresis system that is directly applicable in vivo due to utilization of inert agarose matrix, using a homomultimeric form of VEGF165 to restore the angiogenic balance in preeclampsia. Sepharose 139-146 vascular endothelial growth factor A Homo sapiens 23-27 33061812-5 2020 TERT promoter binding proteins were identified by streptavidin-agarose pulldown assay and mass spectrum (MS) analysis. Sepharose 63-70 telomerase reverse transcriptase Homo sapiens 0-4 32961948-1 2020 The co-immobilization of ketoreductase (KRED) and glucose dehydrogenase (GDH) on highly cross-linked agarose (sepharose) was studied. Sepharose 101-108 hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Homo sapiens 50-71 32961948-1 2020 The co-immobilization of ketoreductase (KRED) and glucose dehydrogenase (GDH) on highly cross-linked agarose (sepharose) was studied. Sepharose 101-108 hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Homo sapiens 73-76 32961948-1 2020 The co-immobilization of ketoreductase (KRED) and glucose dehydrogenase (GDH) on highly cross-linked agarose (sepharose) was studied. Sepharose 110-119 hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Homo sapiens 50-71 32961948-1 2020 The co-immobilization of ketoreductase (KRED) and glucose dehydrogenase (GDH) on highly cross-linked agarose (sepharose) was studied. Sepharose 110-119 hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Homo sapiens 73-76 32473426-2 2020 The 1.5% agarose solution containing the optimal nZVI@biochar dose of 15 g L-1 was used to prepare the nZVI@biochar binding gel which owned a high capacity (1010 +- 50 mug disc-1) and a rapid uptake within 30 min. Sepharose 9-16 L1 cell adhesion molecule Homo sapiens 75-78 32473426-2 2020 The 1.5% agarose solution containing the optimal nZVI@biochar dose of 15 g L-1 was used to prepare the nZVI@biochar binding gel which owned a high capacity (1010 +- 50 mug disc-1) and a rapid uptake within 30 min. Sepharose 9-16 DISC1 scaffold protein Homo sapiens 172-178 32426967-3 2020 Serum protein A1AT was purified from patient sera by immunoprecipitation with anti-A1AT antibody conjugated agarose beads, and the isolated A1AT protein was digested and analyzed by LC-MS/MS. Sepharose 108-115 serpin family A member 1 Homo sapiens 14-18 32563176-5 2020 Specifically, the newly formed DNA-peptide probe was hybridized with miR-224, which was biotinylated and attached to streptavidin agarose in advance. Sepharose 130-137 microRNA 224 Homo sapiens 69-76 32797815-9 2020 The DBC1% has a maximum for the 4 wt% agarose gel, showing optimal tradeoffs between accessibility, specific surface and diffusive mass transfer for IgG. Sepharose 38-45 cell cycle and apoptosis regulator 2 Homo sapiens 4-8 32672313-4 2020 Addition of AAP-FGDS to a covalent, non-responsive agarose network gives rise to a robust hybrid hydrogel with phototunable mechanical properties and shape memory. Sepharose 51-58 serpin family F member 2 Homo sapiens 12-15 32903515-6 2020 Titin isoform expression was analyzed using sodium dodecyl sulfate vertical agarose gel electrophoresis (SDS-VAGE). Sepharose 76-83 titin Rattus norvegicus 0-5 32628020-2 2020 We and others routinely use streptavidin-sepharose matrices to isolate biotinylated polypeptides generated in proximity-dependent biotinylation approaches, such as BioID or APEX. Sepharose 41-50 apurinic/apyrimidinic endodeoxyribonuclease 1 Homo sapiens 173-177 32715101-6 2020 Using an LpMab-7-Sepharose column, RIEDL-wPDPN and CD20-169RIEDL170 were efficiently purified in one-step purification procedures, and were strongly detected by LpMab-7 using Western blot and flow cytometry. Sepharose 17-26 keratin 20 Homo sapiens 51-55 32428679-12 2020 Critically, we show that LCE intracortical probes will deploy within a brain-like agarose tissue phantom. Sepharose 82-89 ELOVL fatty acid elongase 6 Homo sapiens 25-28 32509787-6 2020 Using xanthohumol-sepharose conjugated bead pull-down and mass/mass analysis, we found that KRT18 is a novel target of xanthohumol in KYSE30 cells. Sepharose 18-27 keratin 18 Homo sapiens 92-97 30340444-4 2020 INHalpha c.769G > A mutation was analysed in 100 POI cases and 100 controls using PCR-RFLP and agarose gel electrophoresis. Sepharose 98-105 inhibin subunit alpha Homo sapiens 0-8 32307911-4 2020 Using mass spectrometry and agarose-streptavidin-biotin pull-down analyses, SOX8 (SRY-Box 8) was identified to be the new protein to bind the GOLPH3 promoter within TSCC cells, which was further verified to be the regulator of GOLPH3 upregulation. Sepharose 28-35 SRY-box transcription factor 8 Homo sapiens 76-80 32307911-4 2020 Using mass spectrometry and agarose-streptavidin-biotin pull-down analyses, SOX8 (SRY-Box 8) was identified to be the new protein to bind the GOLPH3 promoter within TSCC cells, which was further verified to be the regulator of GOLPH3 upregulation. Sepharose 28-35 SRY-box transcription factor 8 Homo sapiens 82-91 32307911-4 2020 Using mass spectrometry and agarose-streptavidin-biotin pull-down analyses, SOX8 (SRY-Box 8) was identified to be the new protein to bind the GOLPH3 promoter within TSCC cells, which was further verified to be the regulator of GOLPH3 upregulation. Sepharose 28-35 golgi phosphoprotein 3 Homo sapiens 142-148 32482903-2 2020 Glutathione immobilized on a chromatography matrix, such as agarose or Sepharose, acts as a substrate for the GST moiety of fusion proteins. Sepharose 60-67 glutathione S-transferase kappa 1 Homo sapiens 110-113 32482903-2 2020 Glutathione immobilized on a chromatography matrix, such as agarose or Sepharose, acts as a substrate for the GST moiety of fusion proteins. Sepharose 71-80 glutathione S-transferase kappa 1 Homo sapiens 110-113 32627994-1 2020 A recent study found that an agarose gel electrophoresis (AGE) method yielded two distinct major bands corresponding to the hepatic and bone ALP isoenzymes (ALP2 and ALP3, respec-tively) in bovine serum treated with protease and neuraminidase (PN-treatment), although there were concerns that the intestinal ALP isoenzyme (ALP5) often overlapped with ALP3 in human serum treated with neuraminidase. Sepharose 29-36 neuraminidase 1 Bos taurus 229-242 32627994-1 2020 A recent study found that an agarose gel electrophoresis (AGE) method yielded two distinct major bands corresponding to the hepatic and bone ALP isoenzymes (ALP2 and ALP3, respec-tively) in bovine serum treated with protease and neuraminidase (PN-treatment), although there were concerns that the intestinal ALP isoenzyme (ALP5) often overlapped with ALP3 in human serum treated with neuraminidase. Sepharose 29-36 neuraminidase 1 Bos taurus 384-397 32564742-8 2020 The search for mammalian DING phosphatases in the eluates from thiamine-Sepharose revealed X-DING-CD4, mostly eluted by the acidic thiamine solution (pH 5.6). Sepharose 72-81 ring finger protein 2 Homo sapiens 25-29 32440193-4 2020 Haptoglobin genotype was determined by PCR followed by agarose gel electrophoresis. Sepharose 55-62 haptoglobin Homo sapiens 0-11 32398974-7 2020 Additionally, this technique could detect PCA3 at femtogram level which was approximately 1,000-fold more sensitive than the conventional RT-PCR followed by agarose gel electrophoresis. Sepharose 157-164 prostate cancer associated 3 Homo sapiens 42-46 32209106-3 2020 RESULTS: Using streptavidin-agarose pull-down, we identified the DExD/H-box RNA helicase, DDX39B, as a factor that differentially interacts with kappaB DNA probes. Sepharose 28-35 DExD-box helicase 39B Homo sapiens 90-96 32176842-7 2020 METHODS: The Ca2+/CaM binding to STIM1 was tested by pulling down recombinant GFP-tagged human STIM1 C-terminal fragments on CaM sepharose beads. Sepharose 129-138 stromal interaction molecule 1 Homo sapiens 33-38 32176842-7 2020 METHODS: The Ca2+/CaM binding to STIM1 was tested by pulling down recombinant GFP-tagged human STIM1 C-terminal fragments on CaM sepharose beads. Sepharose 129-138 stromal interaction molecule 1 Homo sapiens 95-100 32564742-8 2020 The search for mammalian DING phosphatases in the eluates from thiamine-Sepharose revealed X-DING-CD4, mostly eluted by the acidic thiamine solution (pH 5.6). Sepharose 72-81 ring finger protein 2 Homo sapiens 93-97 32564742-8 2020 The search for mammalian DING phosphatases in the eluates from thiamine-Sepharose revealed X-DING-CD4, mostly eluted by the acidic thiamine solution (pH 5.6). Sepharose 72-81 CD4 molecule Homo sapiens 98-101 32043104-11 2020 The combination of ITC, agarose gel electrophoresis (AGE) and zeta potential showed that one HSA could bind 8 +- 1 DHLA-AuNCs and one Trf could bind 7 +- 2 DHLA-AuNCs, which was quite different from the conventional model of protein coronas. Sepharose 24-31 albumin Homo sapiens 93-96 32392193-7 2020 In second protocol, anti-Ago2-antibodies are bound to sepharose from the very beginning, and then mixed with plasma. Sepharose 54-63 argonaute RISC catalytic component 2 Homo sapiens 25-29 31537306-0 2020 Protein adsorption to poly(ethylenimine)-modified Sepharose FF: VIII: Impacts of surface ion-exchange groups at different polymer grafting densities. Sepharose 50-59 cytochrome c oxidase subunit 8A Homo sapiens 64-68 31537306-1 2020 Our previous studies on protein adsorption to the anion-exchangers of poly(ethylenimine) (PEI)-grafted Sepharose FF found that both adsorption capacity and uptake rate of bovine serum albumin (BSA) increased greatly when the PEI grafting density reached over a critical ionic capacity (cIC) due to the 3D protein binding and occurrence of "chain delivery" of bound proteins. Sepharose 103-112 albumin Homo sapiens 178-191 31222360-6 2019 Heparin-sepharose chromatography, isothermal titrating calorimetry using fondaparinux as a ligand, and zeta potential measurement indicated that OVAX retained the heparin-binding affinity (Kd = 0.185 +- 0.037) even after 10 D incubation of fertilized egg, although the affinity was slightly decreased during egg incubation because of acidification of molecular surface charge. Sepharose 8-17 ovalbumin-related protein X (SERPINB14C) Gallus gallus 145-149 32515416-5 2020 This was followed by polymerase chain reaction (PCR) with sequence-specific primer of HLA-DQB1*0503 and HLA DRB1*0301 allele, visualised under 2% agarose gel. Sepharose 146-153 major histocompatibility complex, class II, DQ beta 1 Homo sapiens 86-94 31131911-8 2019 For the long T2 agarose phantom, the linear regression slope between UTE-MRF and gold standard was 1.07 (R2 = 0.991) for T1 and 1.04 (R2 = 0.994) for T2 . Sepharose 16-23 myelin regulatory factor Homo sapiens 73-76 31131911-8 2019 For the long T2 agarose phantom, the linear regression slope between UTE-MRF and gold standard was 1.07 (R2 = 0.991) for T1 and 1.04 (R2 = 0.994) for T2 . Sepharose 16-23 interleukin 1 receptor like 1 Homo sapiens 121-129 31782217-0 2020 Rapid and Sensitive Identification of Ricin in Environmental Samples Based on Lactamyl Agarose Beads Using LC-MS/MS (MRM). Sepharose 78-94 ricin Ricinus communis 38-43 31542564-6 2020 In the present study, the peptide Ac-PHQGQHIGVSK contained in the VEGF fragment that binds bevacizumab, was synthesized and immobilized on agarose. Sepharose 139-146 vascular endothelial growth factor A Homo sapiens 66-70 31098824-2 2019 The optimal reaction conditions to produce consistent results were 65 C for 30 min, 63 C for 30 min, and 63 C for 40 min for the groEL (singleplex for V. anguillarum), fklB (singleplex for V. alginolyticus), and groEL + flkB (duplex) LAMP assays, respectively, analyzed via visual detection methods (use of calcein, and SYBR Green I) and agarose gel electrophoresis. Sepharose 341-348 heat shock protein family D (Hsp60) member 1 Homo sapiens 132-137 31393047-4 2019 METHODS: Covalent complexes between ADAMTS13 and VWF were determined by agarose gel electrophoresis under nonreducing conditions. Sepharose 72-79 ADAM metallopeptidase with thrombospondin type 1 motif 13 Homo sapiens 36-44 31393047-4 2019 METHODS: Covalent complexes between ADAMTS13 and VWF were determined by agarose gel electrophoresis under nonreducing conditions. Sepharose 72-79 von Willebrand factor Homo sapiens 49-52 31374157-4 2019 The biocatalyst obtained using Pectinex Ultra SP-L showed the highest thermal stability, at 65 C, and an operational stability of 67% of activity after 10 reuses cycles when immobilized on DVS-agarose immobilized at pH 10 and blocked with ethylenediamine. Sepharose 193-200 sphingosine-1-phosphate lyase 1 Homo sapiens 46-50 31204039-3 2019 To achieve this, we coupled human DNA-clamp proliferating cell nuclear antigen (PCNA) that interacts with over one hundred proteins to an agarose resin. Sepharose 138-145 proliferating cell nuclear antigen Homo sapiens 80-84 31204039-4 2019 We demonstrate the versatility of our PCNA-Agarose column at various chromatographic steps by purifying PCNA-binding proteins that are involved in DNA Replication (DNA polymerase delta, flap endonuclease 1 and DNA ligase 1), translesion DNA synthesis (DNA polymerases eta, kappa and iota) and genome stability (p15). Sepharose 43-50 proliferating cell nuclear antigen Homo sapiens 38-42 31204039-4 2019 We demonstrate the versatility of our PCNA-Agarose column at various chromatographic steps by purifying PCNA-binding proteins that are involved in DNA Replication (DNA polymerase delta, flap endonuclease 1 and DNA ligase 1), translesion DNA synthesis (DNA polymerases eta, kappa and iota) and genome stability (p15). Sepharose 43-50 proliferating cell nuclear antigen Homo sapiens 104-108 31204039-4 2019 We demonstrate the versatility of our PCNA-Agarose column at various chromatographic steps by purifying PCNA-binding proteins that are involved in DNA Replication (DNA polymerase delta, flap endonuclease 1 and DNA ligase 1), translesion DNA synthesis (DNA polymerases eta, kappa and iota) and genome stability (p15). Sepharose 43-50 DNA polymerase delta 1, catalytic subunit Homo sapiens 164-184 31204039-4 2019 We demonstrate the versatility of our PCNA-Agarose column at various chromatographic steps by purifying PCNA-binding proteins that are involved in DNA Replication (DNA polymerase delta, flap endonuclease 1 and DNA ligase 1), translesion DNA synthesis (DNA polymerases eta, kappa and iota) and genome stability (p15). Sepharose 43-50 flap structure-specific endonuclease 1 Homo sapiens 186-205 31204039-4 2019 We demonstrate the versatility of our PCNA-Agarose column at various chromatographic steps by purifying PCNA-binding proteins that are involved in DNA Replication (DNA polymerase delta, flap endonuclease 1 and DNA ligase 1), translesion DNA synthesis (DNA polymerases eta, kappa and iota) and genome stability (p15). Sepharose 43-50 DNA ligase 1 Homo sapiens 210-222 31204039-4 2019 We demonstrate the versatility of our PCNA-Agarose column at various chromatographic steps by purifying PCNA-binding proteins that are involved in DNA Replication (DNA polymerase delta, flap endonuclease 1 and DNA ligase 1), translesion DNA synthesis (DNA polymerases eta, kappa and iota) and genome stability (p15). Sepharose 43-50 endothelin receptor type A Homo sapiens 268-271 31204039-5 2019 We also show the competence of the PCNA-Agarose column to purify non-PCNA binding proteins by fusing the PCNA-binding motif of human p21 as an affinity tag. Sepharose 40-47 proliferating cell nuclear antigen Homo sapiens 35-39 31204039-5 2019 We also show the competence of the PCNA-Agarose column to purify non-PCNA binding proteins by fusing the PCNA-binding motif of human p21 as an affinity tag. Sepharose 40-47 proliferating cell nuclear antigen Homo sapiens 69-73 31204039-5 2019 We also show the competence of the PCNA-Agarose column to purify non-PCNA binding proteins by fusing the PCNA-binding motif of human p21 as an affinity tag. Sepharose 40-47 proliferating cell nuclear antigen Homo sapiens 69-73 31204039-5 2019 We also show the competence of the PCNA-Agarose column to purify non-PCNA binding proteins by fusing the PCNA-binding motif of human p21 as an affinity tag. Sepharose 40-47 H3 histone pseudogene 16 Homo sapiens 133-136 31204039-6 2019 Finally, we establish that our PCNA-Agarose column is a suitable analytical method for characterizing the binding strength of PCNA-binding proteins. Sepharose 36-43 proliferating cell nuclear antigen Homo sapiens 31-35 31204039-6 2019 Finally, we establish that our PCNA-Agarose column is a suitable analytical method for characterizing the binding strength of PCNA-binding proteins. Sepharose 36-43 proliferating cell nuclear antigen Homo sapiens 126-130 31237033-6 2019 Inulin, agarose and cellulose induced IL1beta and MCP1 release, while dextran had no effect on cytokine secretion. Sepharose 8-15 interleukin 1 beta Homo sapiens 38-45 31084743-6 2019 The probe was first hybridized with the target miRNA (i.e., miR-21) that was biotinylated and attached to streptavidin agarose in advance. Sepharose 119-126 microRNA 21 Homo sapiens 60-66 31237033-6 2019 Inulin, agarose and cellulose induced IL1beta and MCP1 release, while dextran had no effect on cytokine secretion. Sepharose 8-15 C-C motif chemokine ligand 2 Homo sapiens 50-54 31002379-11 2019 Moreover, exogenous addition of LPS dose-dependently reduced the amount of LL37 precipitated with the heparin-LL37 agarose beads (P < 0.01), and the released LL37 simultaneously neutralized the pro-inflammatory ability of LPS in macrophages (P < 0.01). Sepharose 115-122 cathelicidin antimicrobial peptide Homo sapiens 75-79 31002379-11 2019 Moreover, exogenous addition of LPS dose-dependently reduced the amount of LL37 precipitated with the heparin-LL37 agarose beads (P < 0.01), and the released LL37 simultaneously neutralized the pro-inflammatory ability of LPS in macrophages (P < 0.01). Sepharose 115-122 cathelicidin antimicrobial peptide Homo sapiens 110-114 31002379-11 2019 Moreover, exogenous addition of LPS dose-dependently reduced the amount of LL37 precipitated with the heparin-LL37 agarose beads (P < 0.01), and the released LL37 simultaneously neutralized the pro-inflammatory ability of LPS in macrophages (P < 0.01). Sepharose 115-122 cathelicidin antimicrobial peptide Homo sapiens 110-114 32227709-5 2019 RESULTS: The more severe the testicular tissue injury, the lower the expression of the STRA8 protein in the SCOS, H-S1 and H-S2 groups as compared with the normal control before in vitro culture on agarose gel (P < 0.05), and the STRA8 expression was significantly upregulated in the former three groups after 4 weeks of culturing (P < 0.05). Sepharose 198-205 stimulated by retinoic acid gene 8 Mus musculus 87-92 31206803-2 2019 Sup35p aggregation yields detergent-resistant assemblies detectable on agarose gels under semi-denaturant conditions and fluorescent puncta within the yeast cytosol when the protein is fused to GFP. Sepharose 71-78 translation termination factor GTPase eRF3 Saccharomyces cerevisiae S288C 0-6 31051245-9 2019 UL23 Polyclonal antibody was purified from antisera using CNBr-activated Sepharose 4 beads. Sepharose 73-82 tegument protein UL23 Human betaherpesvirus 5 0-4 31450568-8 2019 Colony formation and soft agarose assays, testing for anchorage independent growth, revealed that EMP1 overexpressing Y79 cells have a significantly higher ability to form colonies. Sepharose 26-33 epithelial membrane protein 1 Homo sapiens 98-102 32440502-8 2020 The percentage of monocytes in allografts was at a higher level in the spleen and blood; the frequency of CD4+ T cells in the allogeneic group was higher than in the autologous group and the other agarose groups at 6 weeks after transplantation. Sepharose 197-204 Cd4 molecule Rattus norvegicus 106-109 31051203-1 2019 An L-fucose specific lectin from pathogenic fungus Aspergillus niger isolated from the corneal smears of keratitis patient was purified in a single step using mucin coupled sepharose-4B column by 58-fold. Sepharose 173-182 LOC100508689 Homo sapiens 159-164 31029317-0 2019 Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells. Sepharose 63-70 tripartite motif containing 33 Homo sapiens 153-157 32227709-5 2019 RESULTS: The more severe the testicular tissue injury, the lower the expression of the STRA8 protein in the SCOS, H-S1 and H-S2 groups as compared with the normal control before in vitro culture on agarose gel (P < 0.05), and the STRA8 expression was significantly upregulated in the former three groups after 4 weeks of culturing (P < 0.05). Sepharose 198-205 hematopoietic cell specific Lyn substrate 1 Mus musculus 114-118 32227709-5 2019 RESULTS: The more severe the testicular tissue injury, the lower the expression of the STRA8 protein in the SCOS, H-S1 and H-S2 groups as compared with the normal control before in vitro culture on agarose gel (P < 0.05), and the STRA8 expression was significantly upregulated in the former three groups after 4 weeks of culturing (P < 0.05). Sepharose 198-205 hemoglobin, activating region Mus musculus 123-127 30641048-6 2019 In subsequent examinations, P-linked ATP Sepharose was used as an enrichment tool to enable the effective profiling of NLRP3-biomarker signatures with selected reaction monitoring-mass spectrometry (SRM-MS). Sepharose 41-50 NLR family pyrin domain containing 3 Homo sapiens 119-124 30641048-7 2019 Finally, ATP Sepharose was used in combination with a fluorescence-linked enzyme chemoproteomic strategy (FLECS) screen to identify potential competitive inhibitors of NLRP3. Sepharose 13-22 NLR family pyrin domain containing 3 Homo sapiens 168-173 30641048-8 2019 The identification of a novel benzo[d]imidazol-2-one inhibitor that specifically targets the ATP-binding and hydrolysis properties of the NLRP3 protein implies that ATP Sepharose and FLECS could be applied other NLRPs as well. Sepharose 169-178 NLR family pyrin domain containing 3 Homo sapiens 138-143 31366154-4 2019 Genetically programming sGFP and RFP with Cys-tag and His-tag, respectively, allowed tuning the protein spatial organization either across the porous structure of two microbeads with different functional groups (agarose-based materials activated with metal chelates and epoxy-methacrylate materials) or across the surface of a single microbead functionalized with both metal-chelates and disulfide groups. Sepharose 212-219 tripartite motif containing 27 Homo sapiens 33-36 31077676-5 2019 Nondenaturing polyacrylamide gradient gel electrophoresis (GGE), agarose gel electrophoresis and high-performance thin-layer chromatography (HPTLC) indicated that LP3 and DP3 prevented SMase-induced alterations in LDL particle size, electric charge and phospholipid content, respectively, but not those induced by PLA2. Sepharose 65-72 APC regulator of WNT signaling pathway Homo sapiens 171-174 31246422-11 2019 After optimization of the parameters, including temperature, hybridization/DSN time, and the amounts of DSN and streptavidin agarose beads, we demonstrated a linear detection range between 1 fM and 200 fM for miR-200c. Sepharose 125-132 microRNA 200c Homo sapiens 209-217 31311988-2 2019 CF mice harboring the F508del Cftr mutation respond to bacterial challenge with 25,000 CFU Pseudomonas aeruginosa embedded into agarose beads to slow clearance. Sepharose 128-135 cystic fibrosis transmembrane conductance regulator Mus musculus 30-34 31355218-4 2019 The results are as following: LL37, PR39, and IDR-1002 were able to interact with poly(I:C) using an agarose gel migration assay. Sepharose 101-108 cathelicidin antimicrobial peptide Homo sapiens 30-34 31062106-1 2019 Agarose samples of low (Ag1) and high (Ag2) O -methyl content on position 6 of the galactose residue were studied in H2O and D2O. Sepharose 0-7 NBPF member 10 Homo sapiens 24-27 31392083-7 2019 Loss of EDD in MCF-7 cells decreased PRL-induced phosphorylation of eukaryotic initiation factor 4E-binding protein-1, a mediator of TORC1 signaling, resulting in decreased binding of 4E to gamma-aminophenyl-m7GTP agarose in Cap-binding assays. Sepharose 214-221 ubiquitin protein ligase E3 component n-recognin 5 Homo sapiens 8-11 31025537-7 2019 The presence of CYP3A4*1B allele was evaluated on the basis of agarose gel electrophoresis. Sepharose 63-70 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 16-22 30900872-5 2019 Based on these findings, a ratiometric fluorescent-based portable agarose hydrogel test kit was fabricated and applied for on-spot assessment of NO2- content within 10 min. Sepharose 66-73 KIT proto-oncogene, receptor tyrosine kinase Homo sapiens 88-91 30710197-3 2019 In this study, we purified a 65 kDa MNSFbeta adduct from mouse liver lysates by sequential chromatography on DEAE and glutathione S-transferase (GST)-fusioned MNSFbeta immobilized on glutathione-Sepharose beads in the presence of ATP. Sepharose 195-204 Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived) Mus musculus 36-44 30710197-3 2019 In this study, we purified a 65 kDa MNSFbeta adduct from mouse liver lysates by sequential chromatography on DEAE and glutathione S-transferase (GST)-fusioned MNSFbeta immobilized on glutathione-Sepharose beads in the presence of ATP. Sepharose 195-204 hematopoietic prostaglandin D synthase Mus musculus 145-148 31062106-10 2019 This finding implies that the higher hydrophobic effect observed in D2O affects the hydration state much more strongly for the more hydrophobic (and more polarizable) agarose sample Ag2. Sepharose 167-174 anterior gradient 2, protein disulphide isomerase family member Homo sapiens 182-185 30710516-4 2019 Actin-related protein 2/3 complex subunit 2 (ARPC2) was identified as a molecular target of Benp by affinity column chromatography with Benp-tagged Sepharose beads. Sepharose 148-157 actin related protein 2/3 complex subunit 2 Homo sapiens 0-43 30710516-4 2019 Actin-related protein 2/3 complex subunit 2 (ARPC2) was identified as a molecular target of Benp by affinity column chromatography with Benp-tagged Sepharose beads. Sepharose 148-157 actin related protein 2/3 complex subunit 2 Homo sapiens 45-50 31353522-2 2019 TI from duck albumen was isolated and purified using ammonium sulfate precipitation at 20%-40% saturation and affinity chromatography using trypsin-CNBr-activated Sepharose 4B column. Sepharose 163-172 cysteine rich secretory protein LCCL domain containing 2 Homo sapiens 0-2 31091226-5 2019 We confirmed expression of all syndecan subtypes on the endothelial surface of agarose-inflated human lungs. Sepharose 79-86 syndecan 1 Homo sapiens 31-39 30246450-1 2019 The behavior of human immunoglobulin G (IgG) and antigen-binding fragment (Fab fragment) adsorption onto phospho-l-tyrosine immobilized on agarose (P-Tyr-agarose) was evaluated by pseudoaffinity chromatography. Sepharose 139-146 FA complementation group B Homo sapiens 75-78 31080762-4 2019 Then the anti-beta2GPI antibodies were purified by affinity chromatography (Affi-Gel protein A sepharose) and affinity column using human beta2GPI coupled to CNBr-activated Sepharose 4B. Sepharose 95-104 apolipoprotein H Homo sapiens 14-22 31080762-4 2019 Then the anti-beta2GPI antibodies were purified by affinity chromatography (Affi-Gel protein A sepharose) and affinity column using human beta2GPI coupled to CNBr-activated Sepharose 4B. Sepharose 173-182 apolipoprotein H Homo sapiens 14-22 30991766-4 2019 A water-soluble polysaccharide, PD1-1, was successfully obtained from dandelion through ultrasonic-assisted extraction and purification using diethylaminoethyl (DEAE)-Sepharose fast flow and Sephadex G-75 columns. Sepharose 167-176 programmed cell death 1 Homo sapiens 32-37 30850549-8 2019 We also used density gradient ultracentrifugation to characterize the LPL within the high-salt and low-salt peaks from a heparin-Sepharose column. Sepharose 129-138 lipoprotein lipase Homo sapiens 70-73 30731387-2 2019 Analysis of von Willebrand factor (VWF) multimer distribution (VWF:MD) is essential to properly classify and treat different types of VWD, and it is performed using a SDS agarose gel electrophoresis followed by Western blotting, a handmade technique that demands days to be completed and requires skillful execution. Sepharose 171-178 von Willebrand factor Homo sapiens 12-33 30731387-2 2019 Analysis of von Willebrand factor (VWF) multimer distribution (VWF:MD) is essential to properly classify and treat different types of VWD, and it is performed using a SDS agarose gel electrophoresis followed by Western blotting, a handmade technique that demands days to be completed and requires skillful execution. Sepharose 171-178 von Willebrand factor Homo sapiens 35-38 30731387-2 2019 Analysis of von Willebrand factor (VWF) multimer distribution (VWF:MD) is essential to properly classify and treat different types of VWD, and it is performed using a SDS agarose gel electrophoresis followed by Western blotting, a handmade technique that demands days to be completed and requires skillful execution. Sepharose 171-178 von Willebrand factor Homo sapiens 63-66 30112774-4 2019 Approximately 82% electrophoretically homogeneous SCZ IgGs purified using several affinity sorbents including Sepharose with immobilized MBP hydrolyze specifically only MBP but not many other tested proteins. Sepharose 110-119 myelin basic protein Homo sapiens 169-172 30675260-4 2019 Using agarose gel electrophoresis, it was determined that exosome/TRPP2 siRNA complexes were stable in the presence of nucleases and serum. Sepharose 6-13 polycystin 2, transient receptor potential cation channel Homo sapiens 66-71 30280968-2 2019 In this study, using diabetic rat models, a subcutaneous space surrounded with highly vascularized granulomatous tissue was formed by agarose-bFGF rod implantation. Sepharose 134-141 fibroblast growth factor 2 Rattus norvegicus 142-146 30366543-7 2019 The increase in compressive properties and residual weight after the TGA test, combined with the reduction in the swelling percentage of the blend scaffolds suggested an interaction between chitosan and agarose via hydrogen bonding which was confirmed using FTIR analysis. Sepharose 203-210 T-box transcription factor 1 Homo sapiens 69-72 30984154-8 2019 Our new primer set enabled us to correctly identify the VP7 genotypes of representative epidemic strains by agarose gel electrophoresis (G1, G2, human typical G3, equine-like G3, G4, G8, G9, and G12). Sepharose 108-115 VP7 Rotavirus A 56-59 30813492-8 2019 Bt2 was demonstrated by immunoblot when enriched maize endosperm protein with phos-tagTM agarose was in different pollination stages. Sepharose 89-96 Glucose-1-phosphate adenylyltransferase small subunit 2, chloroplastic/amyloplastic/cytosolic Zea mays 0-3 31703162-10 2019 Chromatin immunoprecipitation and streptavidin agarose pull-down assays showed that RUNX2 binds a proximal -400 bp region of the Htra1 promoter during osteogenic differentiation. Sepharose 47-54 runt related transcription factor 2 Mus musculus 84-89 30003689-4 2019 Interactions of ADAM15 and CD95 with calmodulin (CaM), Src, or FAK were analyzed by pull-downs using CaM-Sepharose and coimmunoprecipitations with specific antibodies. Sepharose 105-114 ADAM metallopeptidase domain 15 Homo sapiens 16-22 30003689-4 2019 Interactions of ADAM15 and CD95 with calmodulin (CaM), Src, or FAK were analyzed by pull-downs using CaM-Sepharose and coimmunoprecipitations with specific antibodies. Sepharose 105-114 Fas cell surface death receptor Homo sapiens 27-31 31703162-10 2019 Chromatin immunoprecipitation and streptavidin agarose pull-down assays showed that RUNX2 binds a proximal -400 bp region of the Htra1 promoter during osteogenic differentiation. Sepharose 47-54 HtrA serine peptidase 1 Mus musculus 129-134 30681615-3 2019 The present study was conducted to investigate the association of APOB and patients with FH in a Saudi population.We genotyped 100 patients with FH and 100 controls for 2 polymorphisms in APOB using polymerase chain reaction-restriction fragment length polymorphism, followed by 3% agarose gel electrophoresis. Sepharose 282-289 apolipoprotein B Homo sapiens 188-192 30426432-6 2019 We have used the p19 siRNA binding protein and its cognate dsRNA ligand to demonstrate strategies for identifying optimal conditions to measure apparent binding constants using this agarose gel shift system. Sepharose 182-189 interleukin 23 subunit alpha Homo sapiens 17-20 30320347-5 2019 It was also found that LCH directly bound to Matr3 in a Sepharose 4B pull-down assay. Sepharose 56-65 matrin 3 Homo sapiens 45-50 30716784-4 2019 The promoter region of the COL1A2 gene containing the rs42524 polymorphism was amplified, and the discrimination between the G and C variants was performed by digestion of the polymerase chain reaction (PCR) products with the MspA1I enzyme followed by agarose gel electrophoresis analysis. Sepharose 252-259 collagen type I alpha 2 chain Homo sapiens 27-33 30593382-4 2019 In this study, the porcine GM-CSF (pGM-CSF) and FliC protein were expressed by pSUMO in E.coli Rosetta (DE3) and purified by Ni-NTA Sepharose, respectively. Sepharose 132-141 colony stimulating factor 2 Homo sapiens 27-33 30395435-4 2018 Site-specific covalent immobilization of a methyllysine-binding protein HP1beta chromodomain on the agarose resin was used to purify the methyllysine proteome from the whole-protein mixture. Sepharose 100-107 chromobox 1 Homo sapiens 72-79 30412389-3 2018 Subsequent to trapping FMN in an agarose matrix, light-generated FMN radicals could be produced that were stable for days even under aerobic conditions, and their concentrations were high enough for extensive EPR characterization. Sepharose 33-40 formin 1 Homo sapiens 23-26 30412389-3 2018 Subsequent to trapping FMN in an agarose matrix, light-generated FMN radicals could be produced that were stable for days even under aerobic conditions, and their concentrations were high enough for extensive EPR characterization. Sepharose 33-40 formin 1 Homo sapiens 65-68 30384187-1 2018 Affinity chromatography on procainamide-Sepharose has been an important step in the purification of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) since its introduction in 1978. Sepharose 40-49 butyrylcholinesterase Homo sapiens 100-121 28809090-6 2018 Genotyping for the PSGL-1 gene exon 2 VNTR polymorphism was carried out with the amplification of genomic DNA and running of the polymerase chain reaction product on agarose gel electrophoresis. Sepharose 166-173 selectin P ligand Homo sapiens 19-25 30121300-6 2018 Compared with the monomeric glutaraldehyde agarose (Aga@MG), the dimeric glutaraldehyde agarose (Aga@DG) seems to be involved with more amino acid groups of rSpA during the immobilization. Sepharose 43-50 surfactant protein A1 Rattus norvegicus 157-161 30384187-1 2018 Affinity chromatography on procainamide-Sepharose has been an important step in the purification of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) since its introduction in 1978. Sepharose 40-49 butyrylcholinesterase Homo sapiens 123-127 30384187-1 2018 Affinity chromatography on procainamide-Sepharose has been an important step in the purification of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) since its introduction in 1978. Sepharose 40-49 acetylcholinesterase (Cartwright blood group) Homo sapiens 133-153 30384187-1 2018 Affinity chromatography on procainamide-Sepharose has been an important step in the purification of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) since its introduction in 1978. Sepharose 40-49 acetylcholinesterase (Cartwright blood group) Homo sapiens 155-159 30384187-9 2018 Procainamide-Sepharose will continue to be useful for purification of AChE. Sepharose 13-22 acetylcholinesterase (Cartwright blood group) Homo sapiens 70-74 26876003-4 2018 Chimeric-tPA was purified by lysine-sepharose chromatography and specific aptamers were designed using SELEX method. Sepharose 36-45 plasminogen activator, tissue type Homo sapiens 9-12 30177211-1 2018 A facile one-step radiation method is first developed to synthesize agarose/polyacrylamide (AG/PAM) double-network (DN) hydrogel. Sepharose 68-75 peptidylglycine alpha-amidating monooxygenase Homo sapiens 95-98 30501715-2 2018 METHODS: CITP group and healthy control group were set in this study, The JAK2/STAT3 mRNA expression level in peropheral blood T cells of 2 groups was detected with the RT-PCR and agarose gel electrophoresis. Sepharose 180-187 Janus kinase 2 Homo sapiens 74-78 30501715-2 2018 METHODS: CITP group and healthy control group were set in this study, The JAK2/STAT3 mRNA expression level in peropheral blood T cells of 2 groups was detected with the RT-PCR and agarose gel electrophoresis. Sepharose 180-187 signal transducer and activator of transcription 3 Homo sapiens 79-84 30464462-5 2018 Results: Gel protection assay with agarose gel electrophoresis showed cationic vesicles can protect the shRNA plasmid from DNase 1 enzyme. Sepharose 35-42 deoxyribonuclease 1 Homo sapiens 123-130 30519022-0 2018 Nanoparticle-modified chitosan-agarose-gelatin scaffold for sustained release of SDF-1 and BMP-2. Sepharose 31-38 C-X-C motif chemokine ligand 12 Homo sapiens 81-86 30519022-0 2018 Nanoparticle-modified chitosan-agarose-gelatin scaffold for sustained release of SDF-1 and BMP-2. Sepharose 31-38 bone morphogenetic protein 2 Homo sapiens 91-96 30519022-5 2018 Chitosan-agarose-gelatin (CAG) Scaffolds were then synthesized via gelation technology using cross-linked chitosan, agarose, and gelatin, and were modified by CSO/H NPs. Sepharose 9-16 twist family bHLH transcription factor 1 Homo sapiens 159-168 29913240-4 2018 A novel 19-bp indel mutation was identified in the PLAG1 by sequencing pooled DNA samples (Pool-Seq) and agarose gel electrophoresis methods. Sepharose 105-112 PLAG1 zinc finger Bos taurus 51-56 30346937-5 2018 MAD2 expression was measured through agarose electrophoresis and qt-PCR. Sepharose 37-44 mitotic arrest deficient 2 like 1 Homo sapiens 0-4 29671343-5 2018 Site-directed mutagenesis was used to generate cDNAs encoding these seven SULT2A1 allozymes, which were expressed in BL21 Escherichia coli cells and purified by glutathione-Sepharose affinity chromatography. Sepharose 173-182 sulfotransferase family 2A member 1 Homo sapiens 74-81 30555661-5 2018 Results: The agarose gel electrophoresis showed a189-bp fragment from wild type for both alleles of CCR5 gene. Sepharose 13-20 C-C motif chemokine receptor 5 Homo sapiens 100-104 30214572-3 2018 In the present study, a novel B cell multi-epitope peptide fusion protein (EBV-LMP2-3B), which is composed of three B cell linear epitopes (RIEDPPFNSLL, TLNLT and KSLSSTEFIPN) of EBV latent membrane protein 2 (LMP2), was expressed in a prokaryotic expression system and purified using Ni2+-nitrilotriacetate-Sepharose. Sepharose 308-317 proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2) Mus musculus 79-83 29996006-4 2018 METHODS: We analyzed the MIF-173G/C (rs755622) polymorphism in 180 ALL cases and 150 healthy control children by amplification of the gene using a polymerase chain reaction followed by restriction endonuclease digestion and running on an agarose gel for visualization of the product. Sepharose 238-245 macrophage migration inhibitory factor Homo sapiens 25-28 30568786-4 2018 Here, we report the development of a non-covalent clickable probe, based on SCH772984, a slow off-rate ERK1/2 inhibitor, which enabled efficient pull down of ERK1/2 protein via click reaction with tetrazine tagged agarose beads. Sepharose 214-221 mitogen-activated protein kinase 3 Homo sapiens 103-109 30568786-4 2018 Here, we report the development of a non-covalent clickable probe, based on SCH772984, a slow off-rate ERK1/2 inhibitor, which enabled efficient pull down of ERK1/2 protein via click reaction with tetrazine tagged agarose beads. Sepharose 214-221 mitogen-activated protein kinase 3 Homo sapiens 158-164 29852377-4 2018 Gradients of bone morphogenetic protein 2 in agarose hydrogels were used to spatially direct the osteogenesis of human mesenchymal stem cells and generate robust osteochondral tissue constructs exhibiting a clear mineral transition from bone to cartilage. Sepharose 45-52 bone morphogenetic protein 2 Homo sapiens 13-41 30197422-3 2018 2% agarose gel electrophoresis revealed a predominance of IL-8 in the chronic inflammatory palatine tonsil group compared to tonsillar hyperplasia. Sepharose 3-10 C-X-C motif chemokine ligand 8 Homo sapiens 58-62 30263913-2 2018 We purified BChE from porcine milk by affinity chromatography on Hupresin-Sepharose. Sepharose 74-83 butyrylcholinesterase Homo sapiens 12-16 30149628-6 2018 An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. Sepharose 113-120 microRNA 205 Homo sapiens 99-106 29392568-4 2018 Recombinant SULT2A1 allozymes were expressed in BL21 E. coli cells, and purified using glutathione-sepharose affinity chromatography. Sepharose 99-108 sulfotransferase family 2A member 1 Homo sapiens 12-19 30080912-0 2018 Performance of probe polymerization-conjunction-agarose gel electrophoresis in the rapid detection of KRAS gene mutation. Sepharose 48-55 KRAS proto-oncogene, GTPase Homo sapiens 102-106 30051452-3 2018 The genotyping of 5-HTTLPR was determined by PCR and agarose gel electrophoresis. Sepharose 53-60 solute carrier family 6 member 4 Homo sapiens 18-26 30080912-8 2018 In conclusion, a simple and rapid method was established based on probe polymerization-conjunction-agarose gel electrophoresis for detecting KRAS gene mutations. Sepharose 99-106 KRAS proto-oncogene, GTPase Homo sapiens 141-145 29626075-5 2018 Reverse-transcription polymerase chain reaction and agarose gel analysis found two different forms (variant and wild type) of SULT4A1 mRNA in neurons; the level of wild type correlated with the protein level of SULT4A1. Sepharose 52-59 sulfotransferase family 4A, member 1 Mus musculus 126-133 29625126-9 2018 In the case of cytb, a nested multiplex (single tube PCR) test was designed and successfully tested to differentially detect lineages of Plasmodium and Haemoproteus parasites by yielding amplicons with different sizes detectable in a standard agarose gel. Sepharose 243-250 mitochondrially encoded cytochrome b Homo sapiens 15-19 29943546-6 2018 The experimental investigations of agarose gel diffusion revealed that the recombinant expression modified VIP had relatively strong antibacterial activity to E. coli ATCC25922 and S. aureus ATCC25923. Sepharose 35-42 vasoactive intestinal peptide Rattus norvegicus 107-110 29851469-6 2018 RG7388-TCO was also used to pull down MDM2 by reaction with tetrazine-tagged agarose beads in SJSA-1 lysates. Sepharose 77-84 MDM2 proto-oncogene Homo sapiens 38-42 29627321-4 2018 THP-1 cells were activated while being cultured on 1%, 4%, 10% agarose gel (soft substrate) or on a plastic plate (stiff substrate). Sepharose 63-70 GLI family zinc finger 2 Homo sapiens 0-5 29896649-0 2018 Affinity capture of aflatoxin B1 and B2 by aptamer-functionalized magnetic agarose microspheres prior to their determination by HPLC. Sepharose 75-82 anthocyanin regulatory R-S protein-like Zea mays 30-39 29896649-14 2018 Graphical abstract Schematic of novel aptamer functionalized magnetic agarose microspheres (Apt-MAM) as magnetic adsorbents for simultaneous and specific affinity capture of aflatoxins B1 and B2 (AFBs). Sepharose 70-77 anthocyanin regulatory R-S protein-like Zea mays 185-194 29922592-7 2018 Moreover, a novel protocol for the regeneration of the calmodulin-agarose resin is outlined and validated. Sepharose 66-73 calmodulin Saccharomyces cerevisiae S288C 55-65 29983797-14 2018 Agarose gel analysis of P. falciparumssrRNA gene (206 bp) for 28 specimens containing 10% concordant and discordant samples showed that all 12 negative specimens for RDTs and routine microscopy were truly negative for nPCR. Sepharose 0-7 nasopharyngeal carcinoma-related protein Homo sapiens 218-222 29864125-6 2018 Using a chemoenzymatically modified calmodulin, we employ popular click chemistry reactions for the conjugation of calmodulin to Sepharose resin, thereby streamlining a previously multi-step purification and conjugation process. Sepharose 129-138 calmodulin 1 Homo sapiens 36-46 29864125-6 2018 Using a chemoenzymatically modified calmodulin, we employ popular click chemistry reactions for the conjugation of calmodulin to Sepharose resin, thereby streamlining a previously multi-step purification and conjugation process. Sepharose 129-138 calmodulin 1 Homo sapiens 115-125 29864125-7 2018 We show that this "next-generation" calmodulin-Sepharose resin is not only easy to produce, but is also able to purify more calmodulin-binding proteins per volume of resin than traditional calmodulin-Sepharose resins. Sepharose 47-56 calmodulin 1 Homo sapiens 36-46 29864125-7 2018 We show that this "next-generation" calmodulin-Sepharose resin is not only easy to produce, but is also able to purify more calmodulin-binding proteins per volume of resin than traditional calmodulin-Sepharose resins. Sepharose 47-56 calmodulin 1 Homo sapiens 124-134 29864125-7 2018 We show that this "next-generation" calmodulin-Sepharose resin is not only easy to produce, but is also able to purify more calmodulin-binding proteins per volume of resin than traditional calmodulin-Sepharose resins. Sepharose 47-56 calmodulin 1 Homo sapiens 124-134 29864125-7 2018 We show that this "next-generation" calmodulin-Sepharose resin is not only easy to produce, but is also able to purify more calmodulin-binding proteins per volume of resin than traditional calmodulin-Sepharose resins. Sepharose 200-209 calmodulin 1 Homo sapiens 36-46 29964265-3 2018 It was shown that purification on CNBr-activated Sepharose with immobilized alpha-synuclein resulted in antibody preparation with rabbit serum histidine-rich glycoprotein as a contaminant. Sepharose 49-58 synuclein alpha Homo sapiens 76-91 29318463-9 2018 Agarose gel electrophoresis showed only one wild-type band in the cDNA corresponding to the former group, whereas an extra band was present in samples from the latter group corresponding to the skipping of exon 4a; this confirms that the variant affects PRKAR1A splicing. Sepharose 0-7 protein kinase cAMP-dependent type I regulatory subunit alpha Homo sapiens 254-261 29928576-8 2018 The expression of Col X and Runx2 was increased by compressive loading but suppressed by addition of olmesartan, an Ang II receptor blocker, to the agarose scaffolds. Sepharose 148-155 runt related transcription factor 2 Mus musculus 28-33 29575187-9 2018 A soybean trypsin inhibitor (SBTI) -agarose affinity column was used to independently identify trypsin-like accessory gland proteins. Sepharose 36-43 kunitz trypsin protease inhibitor Glycine max 10-27 29462487-4 2018 Cation-exchange chromatography using SP Sepharose resin showed that alkali treatment (pH 10, 55 C) of OVAX induces the occurrence of a distinct OVAX form with a less positive-charge (acidic OVAX). Sepharose 40-49 ovalbumin-related protein X (SERPINB14C) Gallus gallus 102-106 29462487-4 2018 Cation-exchange chromatography using SP Sepharose resin showed that alkali treatment (pH 10, 55 C) of OVAX induces the occurrence of a distinct OVAX form with a less positive-charge (acidic OVAX). Sepharose 40-49 ovalbumin-related protein X (SERPINB14C) Gallus gallus 144-148 29462487-4 2018 Cation-exchange chromatography using SP Sepharose resin showed that alkali treatment (pH 10, 55 C) of OVAX induces the occurrence of a distinct OVAX form with a less positive-charge (acidic OVAX). Sepharose 40-49 ovalbumin-related protein X (SERPINB14C) Gallus gallus 144-148 29462487-8 2018 Acidic OVAX induced by alkali treatment exhibited weaker interactions with Heparin Sepharose resin than native OVAX did. Sepharose 83-92 ovalbumin-related protein X (SERPINB14C) Gallus gallus 7-11 29652825-5 2018 Subsequently, the lysozyme loaded sericin/agarose composite gel was successfully prepared by the solution impregnation method. Sepharose 42-49 lysozyme Homo sapiens 18-26 29652825-9 2018 So, the lysozyme loaded sericin/agarose gel is a potential alternative biomaterial for wound dressing. Sepharose 32-39 lysozyme Homo sapiens 8-16 29345430-7 2018 The analytical performance of the resulting bandage and microneedle sensing systems are evaluated using TYR-containing agarose phantom gel and porcine skin. Sepharose 119-126 tyrosinase Homo sapiens 104-107 29277934-10 2018 Chromatin IP-PCR and agarose gel electrophoresis were utilized to validate the recruitment of TET1 to its target loci in the FAM20C promoter. Sepharose 21-28 tet methylcytosine dioxygenase 1 Homo sapiens 94-98 29106040-4 2018 The temporal effect of transforming growth factor beta 3 (TGFbeta3) and fibroblast growth factor 2 (FGF2) on the derivation of proliferative chondrocytes from MSCs in three-dimensional agarose was investigated by manipulating the duration of TGFbeta3 and FGF2 treatment. Sepharose 185-192 transforming growth factor beta 3 Homo sapiens 23-56 29106040-4 2018 The temporal effect of transforming growth factor beta 3 (TGFbeta3) and fibroblast growth factor 2 (FGF2) on the derivation of proliferative chondrocytes from MSCs in three-dimensional agarose was investigated by manipulating the duration of TGFbeta3 and FGF2 treatment. Sepharose 185-192 transforming growth factor beta 3 Homo sapiens 58-66 29106040-4 2018 The temporal effect of transforming growth factor beta 3 (TGFbeta3) and fibroblast growth factor 2 (FGF2) on the derivation of proliferative chondrocytes from MSCs in three-dimensional agarose was investigated by manipulating the duration of TGFbeta3 and FGF2 treatment. Sepharose 185-192 fibroblast growth factor 2 Homo sapiens 72-98 29106040-4 2018 The temporal effect of transforming growth factor beta 3 (TGFbeta3) and fibroblast growth factor 2 (FGF2) on the derivation of proliferative chondrocytes from MSCs in three-dimensional agarose was investigated by manipulating the duration of TGFbeta3 and FGF2 treatment. Sepharose 185-192 fibroblast growth factor 2 Homo sapiens 100-104 30581348-7 2018 To confirm the presence of ADA enzyme, the fresh serums, extractions from erythrocytes, JM cell line as a human T lymphocyte line and J774 A.1 as mouse monocyte line were electrophoresed on 1.2% agarose gel and stained with the specific dye. Sepharose 195-202 adenosine deaminase Homo sapiens 27-30 29634395-10 2018 In the present study, recombinant HRP3 was expressed in Escherichia coli and purified with Ni-NTA agarose column. Sepharose 98-105 HDGF like 3 Rattus norvegicus 34-38 29357090-8 2018 Furthermore, the results also indicate that the expression of ABCG2 gene was up-regulated after culture in 2.5% HMC-agarose based culture system. Sepharose 116-123 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 62-67 29301938-5 2018 Using UBF-Sepharose to isolate UBF-binding proteins, we identified histone H1.2 as a candidate partner but were puzzled by this observation, given that UBF is known to be located predominantly in nucleoli, whereas H1.2 distributed broadly among the chromatins in interphase nuclei. Sepharose 10-19 upstream binding transcription factor Homo sapiens 6-9 29301938-5 2018 Using UBF-Sepharose to isolate UBF-binding proteins, we identified histone H1.2 as a candidate partner but were puzzled by this observation, given that UBF is known to be located predominantly in nucleoli, whereas H1.2 distributed broadly among the chromatins in interphase nuclei. Sepharose 10-19 upstream binding transcription factor Homo sapiens 31-34 29301938-5 2018 Using UBF-Sepharose to isolate UBF-binding proteins, we identified histone H1.2 as a candidate partner but were puzzled by this observation, given that UBF is known to be located predominantly in nucleoli, whereas H1.2 distributed broadly among the chromatins in interphase nuclei. Sepharose 10-19 upstream binding transcription factor Homo sapiens 31-34 29334217-3 2018 Using a combination of nuclear magnetic resonance (NMR) titration, isothermal titration calorimetry, fluorescence anisotropy, and native agarose gel electrophoresis, we have identified a direct interaction between the p53 DBD and Hsp90 co-chaperone p23 that occurs in the absence of Hsp90. Sepharose 137-144 tumor protein p53 Homo sapiens 218-221 29334217-3 2018 Using a combination of nuclear magnetic resonance (NMR) titration, isothermal titration calorimetry, fluorescence anisotropy, and native agarose gel electrophoresis, we have identified a direct interaction between the p53 DBD and Hsp90 co-chaperone p23 that occurs in the absence of Hsp90. Sepharose 137-144 heat shock protein 90 alpha family class A member 1 Homo sapiens 230-235 29334217-3 2018 Using a combination of nuclear magnetic resonance (NMR) titration, isothermal titration calorimetry, fluorescence anisotropy, and native agarose gel electrophoresis, we have identified a direct interaction between the p53 DBD and Hsp90 co-chaperone p23 that occurs in the absence of Hsp90. Sepharose 137-144 prostaglandin E synthase 3 Homo sapiens 249-252 29334217-3 2018 Using a combination of nuclear magnetic resonance (NMR) titration, isothermal titration calorimetry, fluorescence anisotropy, and native agarose gel electrophoresis, we have identified a direct interaction between the p53 DBD and Hsp90 co-chaperone p23 that occurs in the absence of Hsp90. Sepharose 137-144 heat shock protein 90 alpha family class A member 1 Homo sapiens 283-288 29334217-5 2018 We show by NMR and native agarose gel electrophoresis that a p53-specific double-stranded DNA sequence competes successfully with p23 for binding to the p53 DBD. Sepharose 26-33 tumor protein p53 Homo sapiens 61-64 29334217-5 2018 We show by NMR and native agarose gel electrophoresis that a p53-specific double-stranded DNA sequence competes successfully with p23 for binding to the p53 DBD. Sepharose 26-33 prostaglandin E synthase 3 Homo sapiens 130-133 29334217-5 2018 We show by NMR and native agarose gel electrophoresis that a p53-specific double-stranded DNA sequence competes successfully with p23 for binding to the p53 DBD. Sepharose 26-33 tumor protein p53 Homo sapiens 153-156 29285662-5 2018 Incubation of Lf with hemin-agarose resulted in negligible binding of Lf with biotinylated hemin. Sepharose 28-35 lactotransferrin Bos taurus 14-16 29285662-5 2018 Incubation of Lf with hemin-agarose resulted in negligible binding of Lf with biotinylated hemin. Sepharose 28-35 lactotransferrin Bos taurus 70-72 29449329-5 2018 Apolipoprotein(a) isoform size, estimated by the number of kringle IV domains, was measured by agarose gel electrophoresis and the predominantly expressed isoform identified. Sepharose 95-102 lipoprotein(a) Homo sapiens 0-16 29630049-5 2018 The amino group functionalized dibutyl phthalate (DBP-NH2) as the anchor target was synthesized and immobilized on the epoxy-activated agarose beads, allowing the display of the phthalic ester group at the surface of the immobilization matrix, and therefore the selection of the group-specific binders. Sepharose 135-142 D-box binding PAR bZIP transcription factor Homo sapiens 50-53 29545609-3 2018 By staining thin (5-10 microm) paraffin and thick (50 microm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Sepharose 62-69 erb-b2 receptor tyrosine kinase 2 Homo sapiens 99-103 29545609-3 2018 By staining thin (5-10 microm) paraffin and thick (50 microm) agarose tissue sections, we detected HER2- and CEA-positive human tumour cells infiltrating the surrounding tissues or metastasizing to different organs, including the brain, testis, lung, liver, and lymph nodes. Sepharose 62-69 CEA cell adhesion molecule 3 Homo sapiens 109-112 33418766-3 2018 The plk-IGF-I Ea was site-specifically immobilized to agarose particles, resulting in homogeneous product outcome with retained potency while providing the necessary tools to maximize local and minimize systemic exposure. Sepharose 54-61 insulin like growth factor 1 Homo sapiens 4-16 31463436-5 2018 We report that preculture of NP cells in agarose gels was required in order for cells to be mechanoresponsive, and this correlated with increased type VI collagen, alpha5beta1 integrin, and fibronectin expression. Sepharose 41-48 fibronectin 1 Homo sapiens 190-201 29505564-5 2018 PLA2 inhibitor was isolated using two chromatographic steps: an ion exchange column (DEAE), followed by an affinity column (crotoxin coupled to a CNBr-activated Sepharose resin). Sepharose 161-170 phospholipase A2 group IIA Homo sapiens 0-4 29350029-3 2018 The microwells are filled with agarose solution, and subsequent addition of hot oil results in immediate formation of agarose droplets due to the surface-tension of the liquid solution. Sepharose 31-38 alcohol dehydrogenase iron containing 1 Homo sapiens 76-79 29350029-3 2018 The microwells are filled with agarose solution, and subsequent addition of hot oil results in immediate formation of agarose droplets due to the surface-tension of the liquid solution. Sepharose 118-125 alcohol dehydrogenase iron containing 1 Homo sapiens 76-79 29577017-4 2018 hMSC rings were engineered by seeding cells with microparticles presenting (1) TGF-beta1, (2) BMP-2, or (3) TGF-beta1 + BMP-2 in custom agarose wells to facilitate self-assembly within 2 d, followed by horizontal culture on glass tubes for 5 weeks. Sepharose 136-143 musculin Homo sapiens 0-4 29577017-4 2018 hMSC rings were engineered by seeding cells with microparticles presenting (1) TGF-beta1, (2) BMP-2, or (3) TGF-beta1 + BMP-2 in custom agarose wells to facilitate self-assembly within 2 d, followed by horizontal culture on glass tubes for 5 weeks. Sepharose 136-143 bone morphogenetic protein 2 Homo sapiens 120-125 29420310-3 2018 Uncompressed and nanostructured fibrin-agarose hydrogels (FAH and NFAH, respectively) have emerged as promising scaffolds in TE, but their structure and biomechanical properties must be improved in order to broaden their TE applications. Sepharose 39-46 fumarylacetoacetate hydrolase Homo sapiens 58-61 29331223-6 2018 Study of the FDH/Tra-Trz-3APP-Sepharose interaction, through adsorption equilibrium studies and site-directed mutagenesis of selected FDH coenzyme binding residues, provided additional experimental evidences of the specificity of the interaction. Sepharose 30-39 alcohol dehydrogenase 5 (class III), chi polypeptide Homo sapiens 13-16 29331223-6 2018 Study of the FDH/Tra-Trz-3APP-Sepharose interaction, through adsorption equilibrium studies and site-directed mutagenesis of selected FDH coenzyme binding residues, provided additional experimental evidences of the specificity of the interaction. Sepharose 30-39 T cell receptor alpha locus Homo sapiens 17-20 29331223-6 2018 Study of the FDH/Tra-Trz-3APP-Sepharose interaction, through adsorption equilibrium studies and site-directed mutagenesis of selected FDH coenzyme binding residues, provided additional experimental evidences of the specificity of the interaction. Sepharose 30-39 alcohol dehydrogenase 5 (class III), chi polypeptide Homo sapiens 134-137 29331223-7 2018 The Tra-Trz-3APP-Sepharose biomimetic adsorbent was further evaluated towards a range of different dehydrogenases and was exploited for the development of a single-step purification protocol for FDH. Sepharose 17-26 T cell receptor alpha locus Homo sapiens 4-7 29331223-7 2018 The Tra-Trz-3APP-Sepharose biomimetic adsorbent was further evaluated towards a range of different dehydrogenases and was exploited for the development of a single-step purification protocol for FDH. Sepharose 17-26 alcohol dehydrogenase 5 (class III), chi polypeptide Homo sapiens 195-198 29288518-3 2018 To confirm the antagonistic system of the recycling C5aR, S-tagged deltaLf-coupled BrCN-activated Sepharose 4B beads were incubated with cytoplasmic proteins and identified a neutrophil-specific deltaANXA3 via pull-down experiments. Sepharose 98-107 complement C5a receptor 1 Homo sapiens 52-56 30081723-8 2018 RhoA activation was determined with a Rhotekin RBD agarose bead-based assay kit. Sepharose 51-58 ras homolog family member A Mus musculus 0-4 29443080-3 2018 It works by soaking the acrylamide or agarose DNA gel in a solution of 1x (equivalent to 2.0 microM) SYBR Green I (SG I) and 0.20 mM nitro blue tetrazolium that produces a purple precipitate of formazan when exposed to sunlight or specifically blue light. Sepharose 38-45 semenogelin 1 Homo sapiens 115-119 29174589-5 2018 Using a focal adhesion kinase (FAK) inhibitor, we demonstrated that focal adhesion signaling is involved in the response of NP cells in hydrogels that contain integrin binding sites (i.e. methacrylated gelatin (gelMA) and type II collagen), but not in hydrogels deplete from integrin binding sites such as alginate and agarose, or CD44-binding hydrogels based on hyaluronic acid. Sepharose 319-326 protein tyrosine kinase 2 Homo sapiens 31-34 29108629-7 2018 Human pepsin C was purified from the culture medium using a Ni-NTA agarose column and the NH2-terminal 5-residue sequences were verified by amino acid sequencing. Sepharose 67-74 progastricsin Homo sapiens 6-14 30735328-6 2018 Also, all samples were tested for mutations in the CALR gene by electrophoretic detection of PCR results in an agarose gel. Sepharose 111-118 calreticulin Homo sapiens 51-55 30141035-0 2018 Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis. Sepharose 84-91 spectrin repeat containing nuclear envelope protein 1 Homo sapiens 46-55 30141035-0 2018 Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis. Sepharose 84-91 spectrin repeat containing nuclear envelope protein 2 Homo sapiens 60-69 30141035-3 2018 Here, we describe the application of vertical agarose gel electrophoresis to identify large isoforms of nesprin-1 and nesprin-2. Sepharose 46-53 spectrin repeat containing nuclear envelope protein 1 Homo sapiens 104-113 30141035-3 2018 Here, we describe the application of vertical agarose gel electrophoresis to identify large isoforms of nesprin-1 and nesprin-2. Sepharose 46-53 spectrin repeat containing nuclear envelope protein 2 Homo sapiens 118-127 29476513-5 2018 These CPE substrates can be purified on an anhydrotrypsin-agarose affinity resin, which specifically binds peptides with C-terminal basic residues. Sepharose 58-65 carboxypeptidase E Mus musculus 6-9 28875213-3 2017 This study reports the identification and characterization of galectin-3 interacting proteins using a combination of galectin-3 sepharose affinity and leucoagglutinating phytohemagglutinin (L-PHA) columns. Sepharose 128-137 galectin 3 Homo sapiens 62-72 28852936-13 2017 Synaptopodin regulates the integrity of cytoskeleton and cell motility of podocytes and this phenomenon was confirmed through scratch assay using agarose molds that showed high cell mobility and migration. Sepharose 146-153 synaptopodin Homo sapiens 0-12 29321362-3 2017 This study was mainly to explore the pathogenic mutation of ADAR1 gene and provide genetics counselling and prenatal diagnostic testing for childbearing individuals.Mutational analysis of ADAR1 gene was performed by polymerase chain reaction (PCR) and electrophoretic separation of PCR products by 1.5% agarose gel electrophoresis. Sepharose 303-310 adenosine deaminase RNA specific Homo sapiens 60-65 29321362-3 2017 This study was mainly to explore the pathogenic mutation of ADAR1 gene and provide genetics counselling and prenatal diagnostic testing for childbearing individuals.Mutational analysis of ADAR1 gene was performed by polymerase chain reaction (PCR) and electrophoretic separation of PCR products by 1.5% agarose gel electrophoresis. Sepharose 303-310 adenosine deaminase RNA specific Homo sapiens 188-193 29091276-4 2017 HBP-tagged fusion proteins can be purified by heparin Sepharose affinity chromatography using a simple sodium chloride gradient to elute the bound fusion protein. Sepharose 54-63 azurocidin 1 Homo sapiens 0-3 28757372-1 2017 Two novel magnetic agarose bead based assays have been developed to measure complement component C5 interaction with C3b and the Factor I Modules (FIMs) of C7. Sepharose 19-26 complement C3 Homo sapiens 117-120 28757372-2 2017 One innovation was to couple C3b onto the magnetic agarose bead using the alternative pathway C3 convertase, which resulted in a linkage of the ligand by a covalent ester bond. Sepharose 51-58 complement C3 Homo sapiens 29-32 28811248-5 2017 Horse IgG3 was purified in a single step on jacalin-Sepharose and absorbed to standard ELISA plates as the capture molecule for reactive human IgE. Sepharose 52-61 immunoglobulin heavy constant gamma 3 (G3m marker) Homo sapiens 6-10 29188619-3 2017 The 5th exon of the TOR1A gene and its flanking sequences were amplified with PCR and analyzed with agarose electrophoresis, fluorescence labeled fragment analysis and Sanger sequencing. Sepharose 100-107 torsin family 1 member A Homo sapiens 20-25 29486703-5 2017 Preparations of purified NEP with either N- or C-terminal (His)6-tag were obtained using Ni-NTA agarose affinity chromatography with yield of more than 20 mg per liter of culture. Sepharose 96-103 membrane metalloendopeptidase Homo sapiens 25-28 28844813-0 2017 Measurement of O-GlcNAcylated endothelial nitric oxide synthase by using 2",5"-ADP-Sepharose pull-down assay. Sepharose 83-92 nitric oxide synthase 3 Rattus norvegicus 30-63 28844813-5 2017 We here report a simplified assay by employing the high binding affinity of eNOS with the 2",5"-ADP-Sepharose resins. Sepharose 100-109 nitric oxide synthase 3 Rattus norvegicus 76-80 29126301-20 2017 Testing the functional activity of VWF, utilizes the drug ristocetin.The state of multimerization of VWF is important and is assessed by electrophoresis on agarose gels. Sepharose 156-163 von Willebrand factor Homo sapiens 35-38 29126301-20 2017 Testing the functional activity of VWF, utilizes the drug ristocetin.The state of multimerization of VWF is important and is assessed by electrophoresis on agarose gels. Sepharose 156-163 von Willebrand factor Homo sapiens 101-104 28980671-5 2017 Importantly, to widen the application of point-of-care testing (POCT) of glucose to biomedical diagnosis, an integrated agarose hydrogel-based sensing platform comprising NCs, GOx and TMB was rationally designed. Sepharose 120-127 hydroxyacid oxidase 1 Homo sapiens 176-179 29049411-4 2017 In this study, we identified XRCC5 as a binding protein of the COX-2 gene promoter in colon cancer cells with streptavidin-agarose pulldown assay and mass spectrometry analysis, and found that XRCC5 promoted colon cancer growth through modulation of COX-2 signaling. Sepharose 123-130 X-ray repair complementing defective repair in Chinese hamster cells 5 Mus musculus 29-34 29049411-4 2017 In this study, we identified XRCC5 as a binding protein of the COX-2 gene promoter in colon cancer cells with streptavidin-agarose pulldown assay and mass spectrometry analysis, and found that XRCC5 promoted colon cancer growth through modulation of COX-2 signaling. Sepharose 123-130 cytochrome c oxidase II, mitochondrial Mus musculus 63-68 29049411-4 2017 In this study, we identified XRCC5 as a binding protein of the COX-2 gene promoter in colon cancer cells with streptavidin-agarose pulldown assay and mass spectrometry analysis, and found that XRCC5 promoted colon cancer growth through modulation of COX-2 signaling. Sepharose 123-130 X-ray repair complementing defective repair in Chinese hamster cells 5 Mus musculus 193-198 29037260-2 2017 METHODS: A novel lamprey immune protein (LIP) as defense molecule was first purified and identified in jawless vertebrates (cyclostomes) using hydroxyapatite column and Q Sepharose Fast Flow column. Sepharose 171-180 CCAAT/enhancer binding protein (C/EBP), beta Mus musculus 41-44 28457438-5 2017 Additionally, interesting pH-responsive swelling capacities were obtained, especially for SAPs based on chitosan and agarose with values up to 110gwater/gSAP. Sepharose 117-124 gamma-secretase activating protein Homo sapiens 153-157 28729354-9 2017 vWF multimers and degradation fragments were quantified with agarose and polyacrylamide gel electrophoresis and immunoblotting. Sepharose 61-68 von Willebrand factor Homo sapiens 0-3 28635559-3 2017 The properties of homemade column were compared with those of the mAb affinity protein G (MPG) agarose high flow, a commercially available column successfully used in capturing polyclonal antibodies. Sepharose 95-102 N-methylpurine DNA glycosylase Homo sapiens 90-93 28610775-6 2017 Cells treated with A23 showed morphological features suggestive of apoptosis, the "latter pattern" in agarose gel, the externalization of phosphatidylserine and caspase-3 and PARP cleavage. Sepharose 102-109 immunoglobulin kappa variable 2-24 Homo sapiens 19-22 28380332-7 2017 The lectin was immobilized in CNBr-activated Sepharose 4B and successfully captured fetuin in solution, demonstrating that this lectin remains active and capable of binding carbohydrates. Sepharose 45-54 alpha-2-HS-glycoprotein Oryctolagus cuniculus 84-90 28557068-2 2017 In this study, purification of a G6PD enzyme was carried out by using rat erythrocytes with a specific activity of 13.7 EU/mg and a yield of 67.7 and 155.6-fold by using 2",5"-ADP Sepharose-4B affinity column chromatography. Sepharose 180-189 glucose-6-phosphate dehydrogenase Rattus norvegicus 33-37 28823280-2 2017 METHODS: The trucated type protein ADAMTS13 of eukaryote-expressed recombinant ADAMTS13-T7 was constructed and was purified by using Ni-NTA agarose. Sepharose 140-147 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 Mus musculus 35-43 28823280-2 2017 METHODS: The trucated type protein ADAMTS13 of eukaryote-expressed recombinant ADAMTS13-T7 was constructed and was purified by using Ni-NTA agarose. Sepharose 140-147 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13 Mus musculus 79-87 28749462-3 2017 We verified the relationship between KRT23 and hTERT in CRC using streptavidin-agarose pulldown and chromatin immunoprecipitation (ChIP) assays. Sepharose 79-86 keratin 23 Homo sapiens 37-42 28839469-5 2017 In this study, we pulled down and identified RBFOX3 (RNA binding protein fox-1 homolog 3) as a novel hTERT promoter-binding protein in HCC cells using biotin-streptavidin-agarose pull-down and proteomics approach, and validated it as a regulatory factor for hTERT signaling and tumor growth in HCCs. Sepharose 171-178 RNA binding fox-1 homolog 3 Homo sapiens 45-51 28839469-5 2017 In this study, we pulled down and identified RBFOX3 (RNA binding protein fox-1 homolog 3) as a novel hTERT promoter-binding protein in HCC cells using biotin-streptavidin-agarose pull-down and proteomics approach, and validated it as a regulatory factor for hTERT signaling and tumor growth in HCCs. Sepharose 171-178 RNA binding fox-1 homolog 3 Homo sapiens 53-88 28839469-5 2017 In this study, we pulled down and identified RBFOX3 (RNA binding protein fox-1 homolog 3) as a novel hTERT promoter-binding protein in HCC cells using biotin-streptavidin-agarose pull-down and proteomics approach, and validated it as a regulatory factor for hTERT signaling and tumor growth in HCCs. Sepharose 171-178 telomerase reverse transcriptase Homo sapiens 101-106 29872636-0 2017 Detection and quantification of the giant protein titin by SDS-agarose gel electrophoresis. Sepharose 63-70 titin Homo sapiens 50-55 29872636-4 2017 The method reported here is a 1% vertical SDS-agarose gel electrophoresis system that can solubilize, detect and quantify various titin isoform sizes. Sepharose 46-53 titin Homo sapiens 130-135 28412343-1 2017 PAP1-A, a novel heteropolysaccharide with an average molecular mass of 1.35x105Da, was isolated from Pteridium aquilinum using a combination of chromatography by DEAE Sepharose Fast Flow and Sepharose 4B. Sepharose 167-176 PDGFA associated protein 1 Mus musculus 0-4 28694296-5 2017 However, the triglycerides in apoC-III-enriched TRLs were hydrolyzed more slowly by free LPL, and the inhibitory effect of apoC-III on triglyceride lipolysis was exaggerated when LPL was bound to GPIHBP1 on the surface of agarose beads. Sepharose 222-229 apolipoprotein C-III Mus musculus 30-38 28694296-5 2017 However, the triglycerides in apoC-III-enriched TRLs were hydrolyzed more slowly by free LPL, and the inhibitory effect of apoC-III on triglyceride lipolysis was exaggerated when LPL was bound to GPIHBP1 on the surface of agarose beads. Sepharose 222-229 apolipoprotein C-III Mus musculus 123-131 28694296-5 2017 However, the triglycerides in apoC-III-enriched TRLs were hydrolyzed more slowly by free LPL, and the inhibitory effect of apoC-III on triglyceride lipolysis was exaggerated when LPL was bound to GPIHBP1 on the surface of agarose beads. Sepharose 222-229 lipoprotein lipase Mus musculus 179-182 28837060-6 2017 The enzyme activity of SPION-bound tPA was determined by digestion of fibrin-containing agarose gels and corresponded to about 74% of free tPA activity. Sepharose 88-95 chromosome 20 open reading frame 181 Homo sapiens 35-38 28701209-6 2017 The localization of nuclear factor kappa B (NF-kappaB) p50/p65 and its binding to the cyclooxygenase 2 (COX-2) promoter were determined using confocal immunofluorescence, a streptavidin-agarose pulldown assay and a chromatin immunoprecipitation (ChIP) assay. Sepharose 186-193 nuclear factor kappa B subunit 1 Homo sapiens 20-42 28701209-6 2017 The localization of nuclear factor kappa B (NF-kappaB) p50/p65 and its binding to the cyclooxygenase 2 (COX-2) promoter were determined using confocal immunofluorescence, a streptavidin-agarose pulldown assay and a chromatin immunoprecipitation (ChIP) assay. Sepharose 186-193 nuclear factor kappa B subunit 1 Homo sapiens 44-53 28701209-6 2017 The localization of nuclear factor kappa B (NF-kappaB) p50/p65 and its binding to the cyclooxygenase 2 (COX-2) promoter were determined using confocal immunofluorescence, a streptavidin-agarose pulldown assay and a chromatin immunoprecipitation (ChIP) assay. Sepharose 186-193 prostaglandin-endoperoxide synthase 2 Homo sapiens 104-109 28706253-5 2017 EPO purification from CCS by anti-EPO antibody coupled Sepharose beads yielded excellent purity with acceptable recovery and was free of glycoform bias. Sepharose 55-64 erythropoietin Homo sapiens 0-3 28432497-0 2017 Immobilization of Ulp1 protease on NHS-activated Sepharose: a useful tool for cleavage of the SUMO tag of recombinant proteins. Sepharose 49-58 SUMO specific peptidase 3 Homo sapiens 18-22 28432497-2 2017 RESULTS: We immobilized Ulp1 on N-hydroxysuccinimide (NHS)-activated Sepharose with a coupling efficiency of 1.7 mg/ml. Sepharose 69-78 SUMO specific peptidase 3 Homo sapiens 24-28 28259758-4 2017 Using affinity chromatography with HAP1-GST protein fragments bound to Sepharose columns, this study identified a number of trafficking-related proteins that bind to HAP1. Sepharose 71-80 huntingtin-associated protein 1 Rattus norvegicus 35-39 28259758-4 2017 Using affinity chromatography with HAP1-GST protein fragments bound to Sepharose columns, this study identified a number of trafficking-related proteins that bind to HAP1. Sepharose 71-80 huntingtin-associated protein 1 Rattus norvegicus 166-170 28318627-7 2017 To investigate whether hydrogen peroxide generated by VAP-1 enzyme reaction enhances neutrophil infiltration, we conducted an under-agarose migration assay with purified human neutrophils. Sepharose 132-139 amine oxidase copper containing 3 Homo sapiens 54-59 28680858-5 2017 We showed that "sterile" agarose bead instillation in rat notably increased lung transcript levels of IL-5 and IL-13 at two post-instillation study-points, day 1 and day 3. Sepharose 25-32 interleukin 5 Rattus norvegicus 102-106 28680858-5 2017 We showed that "sterile" agarose bead instillation in rat notably increased lung transcript levels of IL-5 and IL-13 at two post-instillation study-points, day 1 and day 3. Sepharose 25-32 interleukin 13 Rattus norvegicus 111-116 29088764-6 2017 A pull down analysis performed using sepharose-immobilized CaM showed that CBP501 blocks the interaction between CaM and KRas. Sepharose 37-46 calmodulin 1 Homo sapiens 59-62 28534993-6 2017 Polymerase chain reaction products of 19 TCR alpha variable regions (AV) and 20 TCR beta variable regions (BV) gene families obtained from the CD4+ and CD8+ T cells revealed a clear band following separation by 1.5% agarose gel electrophoresis, and each family exhibited >8 bands following separation by 6% sequencing gel electrophoresis. Sepharose 216-223 T cell receptor beta chain protein Sus scrofa 80-88 29088764-6 2017 A pull down analysis performed using sepharose-immobilized CaM showed that CBP501 blocks the interaction between CaM and KRas. Sepharose 37-46 calmodulin 1 Homo sapiens 113-116 29088764-6 2017 A pull down analysis performed using sepharose-immobilized CaM showed that CBP501 blocks the interaction between CaM and KRas. Sepharose 37-46 KRAS proto-oncogene, GTPase Homo sapiens 121-125 28471113-5 2017 Antibacterial activity of HMGN2 was determined using an agarose diffusion assay and minimum inhibitory concentration (MIC) of HMGN2 was determined by the microdilution broth method. Sepharose 56-63 high mobility group nucleosomal binding domain 2 Homo sapiens 26-31 28365482-0 2017 Distributed vasculogenesis from modular agarose-hydroxyapatite-fibrinogen microbeads. Sepharose 40-47 fibrinogen beta chain Homo sapiens 63-73 28365482-16 2017 In this study, we developed small, non-aggregating agarose-hydroxyapatite-fibrinogen microbeads that contained endothelial cells and fibroblasts. Sepharose 51-58 fibrinogen beta chain Homo sapiens 74-84 28630890-6 2017 VNTR polymorphisms of the DRD4 gene were evaluated by PCR using exon 3-specific primers followed by agarose gel electrophoresis. Sepharose 100-107 dopamine receptor D4 Homo sapiens 26-30 29736383-6 2018 Agarose gel electrophoresis was used to analyze the K-ras gene extracted from the pancreas tissues of experimental rats while hematoxylinand eosin staining was used for histological assay. Sepharose 0-7 KRAS proto-oncogene, GTPase Rattus norvegicus 52-57 29736383-10 2018 While the agarose gel electrophoresis revealed that there may be possibility of prevention of damage to k-ras gene as a result of the effect of plants extract. Sepharose 10-17 KRAS proto-oncogene, GTPase Rattus norvegicus 104-109 28041555-0 2017 Agarose-chitosan-C18 film micro-solid phase extraction combined with high performance liquid chromatography for the determination of phenanthrene and pyrene in chrysanthemum tea samples. Sepharose 0-7 Bardet-Biedl syndrome 9 Homo sapiens 17-20 28041555-1 2017 Agarose-chitosan-immobilized octadecylsilyl-silica (C18) film micro-solid phase extraction (muSPE) was developed and applied for the determination of phenanthrene (PHE) and pyrene (PYR) in chrysanthemum tea samples using high performance liquid chromatography-ultraviolet detection (HPLC-UV). Sepharose 0-7 Bardet-Biedl syndrome 9 Homo sapiens 52-55 28041555-2 2017 The film of blended agarose and chitosan allows good dispersion of C18, prevents the leaching of C18 during application and enhances the film mechanical stability. Sepharose 20-27 Bardet-Biedl syndrome 9 Homo sapiens 67-70 28041555-2 2017 The film of blended agarose and chitosan allows good dispersion of C18, prevents the leaching of C18 during application and enhances the film mechanical stability. Sepharose 20-27 Bardet-Biedl syndrome 9 Homo sapiens 97-100 28153532-8 2017 FTY720 also restored BDNF mRNA in OLN-93 cells treated with recombinant aSyn, as measured by qPCR or semiquantitatively on agarose gels. Sepharose 123-130 synuclein alpha Rattus norvegicus 72-76 28024793-7 2017 RESULTS: Antibodies from type 1 diabetes sera, purified in a protein A-agarose matrix, exhibited greater recognition of hrIFN alpha-2b than IFN alpha-2b (p<0.05) and cIFN alpha-2b gene (p<0.001). Sepharose 71-78 interferon alpha 2 Homo sapiens 122-131 28161904-5 2017 Following the analysis of agarose gel spots, cofilin-1 was identified and verified as a candidate protein commonly upregulated in PDAC tissues. Sepharose 26-33 cofilin 1 Homo sapiens 45-54 28163251-8 2017 Moreover, the CES inhibition assay and microcystin-agarose pull down assay showed the possibility of the interaction between CES2 and microcystin-LR. Sepharose 51-58 carboxylesterase 2H Mus musculus 125-129 28114763-5 2017 These ideas are validated in agarose gels loaded with latex particles stabilized by adsorbed bovine serum albumin. Sepharose 29-36 albumin Homo sapiens 100-113 28438863-5 2017 p16 and MDM2 polymorphisms were determined by polymerase chain reaction-restriction fragment polymorphism and agarose gel electrophoresis. Sepharose 110-117 cyclin dependent kinase inhibitor 2A Homo sapiens 0-3 28188826-5 2017 The untagged TET2 enzyme was purified using cation exchange and heparin sepharose chromatography. Sepharose 72-81 tet methylcytosine dioxygenase 2 Homo sapiens 13-17 28153290-5 2017 The unpurified GST-hPPARgammaLBD was directly applied to a 96-well filter plate prepacked with glutathione sepharose. Sepharose 107-116 glutathione S-transferase kappa 1 Homo sapiens 15-18 27768832-8 2017 Receptor associated protein 80 (RAP-80) ubiquitin interacting motif agarose was used in a pull-down assay to obtain K63 -polyubiquitinated proteins. Sepharose 68-75 ubiquitin interaction motif containing 1 Mus musculus 0-30 27768832-8 2017 Receptor associated protein 80 (RAP-80) ubiquitin interacting motif agarose was used in a pull-down assay to obtain K63 -polyubiquitinated proteins. Sepharose 68-75 ubiquitin interaction motif containing 1 Mus musculus 32-38 28198985-3 2017 Here, we use agarose wells to generate uniformly sized mesenchymal stromal cell (MSC) aggregates. Sepharose 13-20 musculin Homo sapiens 81-84 28216573-5 2017 The anti-HER2 diaffibody has been expressed as a His-tagged protein in E. coli and purified by Ni-nitrilotriacetyl (Ni-NTA) agarose chromatography. Sepharose 124-131 erb-b2 receptor tyrosine kinase 2 Homo sapiens 9-13 27840054-4 2017 It works by soaking the acrylamide or agarose DNA gel in SGI and nitro blue tetrazolium (NBT) solution that, when exposed to sunlight, produces a purple insoluble formazan precipitate that remains in the gel after exposure to light. Sepharose 38-45 semenogelin 1 Homo sapiens 57-60 28000561-9 2017 RESULTS: Preliminary screening of amplified products on Agarose gel showed expected size of ~893 bp of PCR product corresponding to RGN. Sepharose 56-63 regucalcin Bubalus bubalis 132-135 29022645-6 2017 MATERIAL AND METHODS: TRIP11 subcellular localization was analyzed using immunocytochemistry in RCC-derived cell line treated with T3, T3-agarose and PI3K inhibitor, wortmannin. Sepharose 138-145 thyroid hormone receptor interactor 11 Homo sapiens 22-28 28695522-2 2017 After cell lysis, PARP-3 protein from the crude extract is affinity purified on a 3-aminobenzamide Sepharose chromatographic step. Sepharose 99-108 poly(ADP-ribose) polymerase family member 3 Homo sapiens 18-24 28695523-2 2017 After cell lysis, GST-PARG-fusion proteins from the crude extract are affinity purified by a Glutathione 4B Sepharose chromatographic step. Sepharose 108-117 poly(ADP-ribose) glycohydrolase Homo sapiens 22-26 27730557-2 2017 MBP allows the use of a simple capture affinity step on Amylose-Agarose or Dextrin-Sepharose columns, resulting in a protein that is often 70-90 % pure in a single step. Sepharose 64-71 myelin basic protein Homo sapiens 0-3 27730557-2 2017 MBP allows the use of a simple capture affinity step on Amylose-Agarose or Dextrin-Sepharose columns, resulting in a protein that is often 70-90 % pure in a single step. Sepharose 83-92 myelin basic protein Homo sapiens 0-3 27884430-6 2016 The platen indenter was applied with 20% strain on the agarose gel in the Mach-1 micromechanical system. Sepharose 55-62 up-regulated in Myc liver Mus musculus 74-80 27811232-4 2017 Indeed, we found that LPL detaches from HSPGs on cultured cells and moves to: 1) soluble GPIHBP1 in the cell culture medium; 2) GPIHBP1-coated agarose beads; and 3) nearby GPIHBP1-expressing cells. Sepharose 143-150 lipoprotein lipase Mus musculus 22-25 27811232-4 2017 Indeed, we found that LPL detaches from HSPGs on cultured cells and moves to: 1) soluble GPIHBP1 in the cell culture medium; 2) GPIHBP1-coated agarose beads; and 3) nearby GPIHBP1-expressing cells. Sepharose 143-150 GPI-anchored HDL-binding protein 1 Mus musculus 128-135 27811232-4 2017 Indeed, we found that LPL detaches from HSPGs on cultured cells and moves to: 1) soluble GPIHBP1 in the cell culture medium; 2) GPIHBP1-coated agarose beads; and 3) nearby GPIHBP1-expressing cells. Sepharose 143-150 GPI-anchored HDL-binding protein 1 Mus musculus 128-135 27811232-6 2017 To test the mobility of HSPG-bound LPL in vivo, we injected GPIHBP1-coated agarose beads into the brown adipose tissue of GPIHBP1-deficient mice. Sepharose 75-82 GPI-anchored HDL-binding protein 1 Mus musculus 60-67 26763308-5 2016 PON1 enzyme was prepared from the serum pool of BC patients using hydrophobic interaction chromatography on L-tyrosine-9-aminophenanthrene-coupled Sepharose 4Bgel. Sepharose 147-156 paraoxonase 1 Homo sapiens 0-4 26887799-2 2016 Human serum paraoxonase 1 was purified from fresh human serum blood by two-step procedures that are ammonium sulfate precipitation (60-80%) and then hydrophobic interaction chromatography (Sepharose 4B, L-tyrosine and 1-napthylamine). Sepharose 189-201 paraoxonase 1 Homo sapiens 12-25 27932754-8 2016 : Results: Agarose gel electrophoresis and DNA sequencing showed that recombinant lentivirus plasmids pITA-RFX1 were constructed successfully. Sepharose 12-19 regulatory factor X1 Rattus norvegicus 108-112 27983990-5 2016 Here, a simple and efficient selective reduction of the single disulfide bond linking the partial heavy chain and the intact light chain which compose the Fab fragment is accomplished utilizing tris(2-carboxyethyl)phosphine (TCEP) immobilized on agarose beads. Sepharose 246-253 FA complementation group B Homo sapiens 155-158 27595549-6 2016 Copper-catalyzed (CuAAC) and copper-free strain-promoted (SPAAC) 1,3-dipolar azide-alkyne cycloadditions were used to site-selectively anchor IL-4 to agarose surfaces. Sepharose 150-157 interleukin 4 Homo sapiens 142-146 27663728-7 2016 A 3-fold higher dynamic binding capacity at 100% breakthrough (DBC100%) was observed for Fab wild-type (wt) on CNBr-activated Sepharose 4 FF relative to mAb on same resin at the same ligand density. Sepharose 126-135 FA complementation group B Homo sapiens 89-92 27655676-4 2016 Using an under agarose chemotaxis assay, we observed that the bacterial fMLP-induced neutrophil chemotaxis signal overrode interleukin 8 (IL-8)- and leukotriene B4 (LTB4)-induced chemotaxis signals. Sepharose 15-22 formyl peptide receptor 1 Homo sapiens 72-76 27655676-4 2016 Using an under agarose chemotaxis assay, we observed that the bacterial fMLP-induced neutrophil chemotaxis signal overrode interleukin 8 (IL-8)- and leukotriene B4 (LTB4)-induced chemotaxis signals. Sepharose 15-22 C-X-C motif chemokine ligand 8 Homo sapiens 123-136 27655676-4 2016 Using an under agarose chemotaxis assay, we observed that the bacterial fMLP-induced neutrophil chemotaxis signal overrode interleukin 8 (IL-8)- and leukotriene B4 (LTB4)-induced chemotaxis signals. Sepharose 15-22 C-X-C motif chemokine ligand 8 Homo sapiens 138-142 27110677-5 2016 MAVS aggregation was detected by semidenatured agarose gel electrophoresis and confirmed by immunofluorescence staining. Sepharose 47-54 mitochondrial antiviral signaling protein Homo sapiens 0-4 27399165-6 2016 The mean Hb E proportions in the Hb E heterozygotes were 23.25+-4.13% and 24.72+-3.5% as determined by Q Sepharose micro-column chromatography and HPLC, respectively. Sepharose 105-114 hemoglobin subunit epsilon 1 Homo sapiens 9-13 27399165-6 2016 The mean Hb E proportions in the Hb E heterozygotes were 23.25+-4.13% and 24.72+-3.5% as determined by Q Sepharose micro-column chromatography and HPLC, respectively. Sepharose 105-114 hemoglobin subunit epsilon 1 Homo sapiens 33-37 27377460-1 2016 A water-soluble polysaccharide (BP-1) was obtained from highland barley (Hordeum vulgare L.) by hot water extraction and purification of sepharose column chromatography. Sepharose 137-146 BP1 Homo sapiens 32-36 27497820-1 2016 Osteocytic potentiality of human CD34+ stem cells explored in the present study by generating in vitro agarose gel 3D model to understand the bone ossification process. Sepharose 103-110 CD34 molecule Homo sapiens 33-37 27474620-1 2016 In a rapid one-step method protein-mimicking large agarose amino acid framework (AAE; GPC 156.7kDa) was conjugated with polyethylene glycol (PEG 9kDa) affording nano-sized PEGylated amphoteric agarose (PEG-AAE; <10nm; DLS) containing amino, carboxyl and ester groups [overall degree of substitution (DS) 0.91]. Sepharose 51-58 glycophorin C (Gerbich blood group) Homo sapiens 86-89 27474620-1 2016 In a rapid one-step method protein-mimicking large agarose amino acid framework (AAE; GPC 156.7kDa) was conjugated with polyethylene glycol (PEG 9kDa) affording nano-sized PEGylated amphoteric agarose (PEG-AAE; <10nm; DLS) containing amino, carboxyl and ester groups [overall degree of substitution (DS) 0.91]. Sepharose 193-200 glycophorin C (Gerbich blood group) Homo sapiens 86-89 27630136-9 2016 Titin isoform expression was evaluated with agarose gels. Sepharose 44-51 titin Mus musculus 0-5 27803781-7 2016 MATERIALS AND METHODS: EPO antibody was covalently crosslinked to protein A/G agarose. Sepharose 78-85 erythropoietin Mus musculus 23-26 27756327-13 2016 Pull down assay using esculetin conjugated sepharose beads confirmed the binding between esculetin and KEAP1. Sepharose 43-52 kelch like ECH associated protein 1 Homo sapiens 103-108 27633629-0 2016 A Novel Assay for Screening Inhibitors Targeting HIV Integrase LEDGF/p75 Interaction Based on Ni(2+) Coated Magnetic Agarose Beads. Sepharose 117-124 PC4 and SFRS1 interacting protein 1 Homo sapiens 69-72 27626280-8 2016 Soft agarose and in ovo chicken chorioallantoic membrane (CAM) assays revealed that TFF3 overexpression influences anchorage independent growth and significantly decreases the size of tumors forming from retinoblastoma cells. Sepharose 5-12 trefoil factor 3 Homo sapiens 84-88 27613595-9 2016 The IHC results of the cell line samples and xenograft tumor samples were as expected, but the staining level of the agarose gel samples, using HER2-overexpressed cell lines was weak which might be regarded as a false negative result. Sepharose 117-124 erb-b2 receptor tyrosine kinase 2 Homo sapiens 144-148 27567377-0 2016 Bovine pancreatic trypsin inhibitor immobilized onto sepharose as a new strategy to purify a thermostable alkaline peptidase from cobia (Rachycentron canadum) processing waste. Sepharose 53-62 trophoblast Kunitz domain protein 1 Bos taurus 18-35 27567377-1 2016 A thermostable alkaline peptidase was purified from the processing waste of cobia (Rachycentron canadum) using bovine pancreatic trypsin inhibitor (BPTI) immobilized onto Sepharose. Sepharose 171-180 spleen trypsin inhibitor I Bos taurus 148-152 27651634-8 2016 The present study of p53 gene regulation analyzed the expression of 279-bp bands on 1.5 agarose gel. Sepharose 88-95 tumor protein p53 Homo sapiens 21-24 27680194-3 2016 Currently, uncompressed and nanostructured fibrin-agarose hydrogels (FAH and NFAH, respectively) have been used successfully in tissue engineering. Sepharose 50-57 fumarylacetoacetate hydrolase Homo sapiens 69-72 27760630-3 2016 Expression of SOX5 in the semen samples was detected and quantified using agarose gel electrophoresis and real time-quantitative PCR (RT-qPCR) analysis. Sepharose 74-81 SRY-box transcription factor 5 Homo sapiens 14-18 27161295-7 2016 A pull-down assay with N-hydroxysuccinimide-activated sepharose identified IgE specific for CTRX only in the serum obtained from the case of CTRX exposure, and not in the control case. Sepharose 54-63 immunoglobulin heavy constant epsilon Homo sapiens 75-78 27264950-3 2016 By RT-PCR/agarose gel electrophoresis of hepcidin mRNA in a hepatocellular carcinoma cell line HLF, a smaller mRNA band was shown in addition to the wild-type hepcidin mRNA. Sepharose 10-17 hepcidin antimicrobial peptide Homo sapiens 41-49 27459679-2 2016 Retention of positive attributes of the agarose standard and nullification of its negatives are central to the current agarose/silk fibroin hydrogel design. Sepharose 119-126 fibroin light chain Bombyx mori 132-139 27494251-3 2016 In performing tissue-specific knockdown with the Gal4-UAS system we identified a new behavioral phenotype for lov: larvae failed to burrow into their food during their growth phase and then failed to tunnel into an agarose substratum during their wandering phase. Sepharose 215-222 jim lovell Drosophila melanogaster 110-113 27499645-1 2016 PURPOSE: Agarose macrobeads containing mouse renal adenocarcinoma cells (RMBs) release factors, suppressing the growth of cancer cells and prolonging survival in spontaneous or induced tumor animals, mediated, in part, by increased levels of myocyte-enhancing factor (MEF2D) via EGFR-and AKT-signaling pathways. Sepharose 9-16 myocyte enhancer factor 2D Mus musculus 268-273 27499645-1 2016 PURPOSE: Agarose macrobeads containing mouse renal adenocarcinoma cells (RMBs) release factors, suppressing the growth of cancer cells and prolonging survival in spontaneous or induced tumor animals, mediated, in part, by increased levels of myocyte-enhancing factor (MEF2D) via EGFR-and AKT-signaling pathways. Sepharose 9-16 epidermal growth factor receptor Mus musculus 279-283 27499645-1 2016 PURPOSE: Agarose macrobeads containing mouse renal adenocarcinoma cells (RMBs) release factors, suppressing the growth of cancer cells and prolonging survival in spontaneous or induced tumor animals, mediated, in part, by increased levels of myocyte-enhancing factor (MEF2D) via EGFR-and AKT-signaling pathways. Sepharose 9-16 thymoma viral proto-oncogene 1 Mus musculus 288-291 27060066-7 2016 cDNA representing BRCA2 alternate splice sites was amplified and visualised using capillary or agarose gel electrophoresis, followed by sequencing. Sepharose 95-102 BRCA2 DNA repair associated Homo sapiens 18-23 27092510-3 2016 When a dye-tethered and prephosphorylated (primed) peptide substrate for GSK3 was employed, a distinct mobility shift in the fluorescent bands on the agarose was observed by GSK3-induced phosphorylation of the primed peptides. Sepharose 150-157 glycogen synthase kinase 3 beta Mus musculus 73-77 27505250-5 2016 By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Sepharose 155-162 CD44 molecule (Indian blood group) Homo sapiens 47-51 27460474-6 2016 RESULTS: The number of different alleles detected for the msp2 gene by resolving PCR products on agarose gels was 14. Sepharose 97-104 merozoite surface protein 2 Plasmodium falciparum 3D7 58-62 27063248-6 2016 Here, the affinity of GST for GSH was used to generate an enzyme-substrate site-specific cross-linking reaction; GSH-Sepharose was preactivated with 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and then incubated Fc gamma receptor IIIa (FcgammaRIIIa)-GST. Sepharose 117-126 glutathione S-transferase kappa 1 Homo sapiens 22-25 27063248-6 2016 Here, the affinity of GST for GSH was used to generate an enzyme-substrate site-specific cross-linking reaction; GSH-Sepharose was preactivated with 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and then incubated Fc gamma receptor IIIa (FcgammaRIIIa)-GST. Sepharose 117-126 Fc gamma receptor IIIa Homo sapiens 218-240 27063248-6 2016 Here, the affinity of GST for GSH was used to generate an enzyme-substrate site-specific cross-linking reaction; GSH-Sepharose was preactivated with 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and then incubated Fc gamma receptor IIIa (FcgammaRIIIa)-GST. Sepharose 117-126 Fc gamma receptor IIIa Homo sapiens 242-254 27063248-6 2016 Here, the affinity of GST for GSH was used to generate an enzyme-substrate site-specific cross-linking reaction; GSH-Sepharose was preactivated with 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and then incubated Fc gamma receptor IIIa (FcgammaRIIIa)-GST. Sepharose 117-126 glutathione S-transferase kappa 1 Homo sapiens 256-259 27405731-3 2016 METHODS: A 126-bp fragment of PIK3CA exon-20 was amplified by PCR, digested with FspI restriction endonuclease and separated by 3 % agarose gel electrophoresis for the PCR-RFLP analysis. Sepharose 132-139 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Homo sapiens 30-36 27110670-6 2016 The collected bacteria lysate was loaded on biotinylated agarose to proceed the purification of CD47-SA fusion protein. Sepharose 57-64 CD47 molecule Homo sapiens 96-100 27470963-6 2016 Agarose gel electrophoresis was used to analyze the binding ability of FA-PEG-PEI/MNP-CDDP to TFPI-2 plasmid. Sepharose 0-7 tissue factor pathway inhibitor 2 Homo sapiens 94-100 27470963-14 2016 Agarose gel electrophoresis showed TFPI-2 could be encapsulated completely and protected from digestion of DNA enzyme as the mass ratio of FA-PEG-PEI/ MNP-CDDP and TFPI-2 plasmid was equal or higher than 1:1. Sepharose 0-7 tissue factor pathway inhibitor 2 Homo sapiens 35-41 26733178-6 2016 Agarose gel electrophoresis assay showed that MBiRGD/CCR2 can effectively load pGPU6/GFP/Neo-shAKT2 plasmid DNA. Sepharose 0-7 C-C motif chemokine receptor 2 Homo sapiens 53-57 27341489-0 2016 Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins. Sepharose 6-13 LOC100508689 Homo sapiens 0-5 27341489-10 2016 Here, we describe conventional methods to separate mucin macromolecules by electrophoresis using an agarose gel, transfer protein into nitrocellulose membrane, and detect signal with mucin-specific antibodies as well as infrared fluorescent gel reader. Sepharose 100-107 LOC100508689 Homo sapiens 51-56 27544994-3 2016 The expression of MIF was detected via immunofluorescence and agarose gel electrophoresis. Sepharose 62-69 macrophage migration inhibitory factor (glycosylation-inhibiting factor) Mus musculus 18-21 27275095-2 2016 METHODS: bFGF mAb was prepared by using the 1G9B9 hybridoma cell line with hybridization technology and extracted from ascites fluid through a Protein G Sepharose affinity column. Sepharose 153-162 fibroblast growth factor 2 Mus musculus 9-13 33440481-2 2016 These cationic glycopolymers were found to form very stable polyplexes with EGFR siRNA as determined by dynamic light scattering and agarose gel electrophoresis. Sepharose 133-140 epidermal growth factor Homo sapiens 76-80 27173315-11 2016 After RT-PCR, clear bands representing SRY, LEP, and RPS23 in sperm cDNA were observed on agarose gel electrophoresis. Sepharose 90-97 sex-determining region Y protein Bos taurus 39-42 27173315-11 2016 After RT-PCR, clear bands representing SRY, LEP, and RPS23 in sperm cDNA were observed on agarose gel electrophoresis. Sepharose 90-97 leptin Bos taurus 44-47 27173315-11 2016 After RT-PCR, clear bands representing SRY, LEP, and RPS23 in sperm cDNA were observed on agarose gel electrophoresis. Sepharose 90-97 40S ribosomal protein S23 Bos taurus 53-58 27040683-6 2016 In a patient with Waldenstrom"s macroglobulinemia and severe depletion of plasma VWF, multimer analysis indicated association of the IgM paraprotein with VWF before, but not after plasmapheresis, resulting in destruction of the agarose gel and a characteristically distorted band structure of VWF multimers. Sepharose 228-235 von Willebrand factor Homo sapiens 154-157 27040683-6 2016 In a patient with Waldenstrom"s macroglobulinemia and severe depletion of plasma VWF, multimer analysis indicated association of the IgM paraprotein with VWF before, but not after plasmapheresis, resulting in destruction of the agarose gel and a characteristically distorted band structure of VWF multimers. Sepharose 228-235 von Willebrand factor Homo sapiens 154-157 26961845-2 2016 The DNA binding and cleavage properties of the complex investigated by viscosity, agarose gel electrophoresis and multi-spectroscopic techniques (UV, circular dichroism (CD) and fluorescence) showed that the complex was bound to CT-DNA through intercalation mode with moderate binding constant (K b = 3.132 x 10(4) M(-1)), and cleaved pBR322 DNA efficiently (~ 5 muM) in the presence of Vc, probably via an oxidative mechanism induced by OH. Sepharose 82-89 translocator protein Homo sapiens 335-338 26826315-2 2016 The steps involve removal of the cryoglobulin and the vitamin K dependent proteins followed by polyethylene glycol and ammonium sulfate precipitations, DEAE-Sephadex column chromatography and finally Factor H-Sepharose affinity purification. Sepharose 209-218 complement factor H Homo sapiens 200-208 27052822-3 2016 Furthermore, it is also presented a novel sample pretreatment based on immunoprecipitation (IP) using Protein A Ultrarapid Agarose (UAPA) magnetic beads (MBs) to purify TTR from serum samples. Sepharose 123-130 transthyretin Homo sapiens 170-173 27114872-9 2016 The 63-bp deletion, a polymorphism in the RAGE gene promoter, was genotyped using conventional PCR method and visualized using agarose gel electrophoresis. Sepharose 127-134 advanced glycosylation end-product specific receptor Homo sapiens 42-46 27016912-4 2016 MMP-9 was expressed in Escherichia coli BL21 and purified by Ni-NTA agarose column. Sepharose 68-75 matrix metallopeptidase 9 Homo sapiens 0-5 27110670-0 2016 Purification of CD47-streptavidin fusion protein from bacterial lysate using biotin-agarose affinity chromatography. Sepharose 84-91 CD47 molecule Homo sapiens 16-20 27184911-2 2016 Regions of interest of KRAS exon 2 were amplified and visualized on 2% agarose gel. Sepharose 71-78 KRAS proto-oncogene, GTPase Homo sapiens 23-27 27144520-6 2016 The under agarose migration assay demonstrated that LPS stimulation promoted migration to the ligand of FPR1, N-Formyl-Met-Leu-Phe (fMLP) but that CORM-2 treatment inhibited this promotion. Sepharose 10-17 formyl peptide receptor 1 Homo sapiens 104-108 26875090-4 2016 Chitinase activity and PGIP2 inhibition were detected by colorimetric and agarose diffusion assay in transgenic lines but not in untransformed plants. Sepharose 74-81 basic endochitinase CHB4 Brassica napus 0-9 26875090-4 2016 Chitinase activity and PGIP2 inhibition were detected by colorimetric and agarose diffusion assay in transgenic lines but not in untransformed plants. Sepharose 74-81 polygalacturonase inhibitor 1-like Brassica napus 23-28 27067900-5 2016 The HD6 antibody was immobilized on an activated sepharose affinity column, and HLA-B27 homodimer characterized for affinity. Sepharose 49-58 defensin alpha 6 Homo sapiens 4-7 26892535-0 2016 Purification of F plasmid-encoded native TraC from Escherichia coli by affinity chromatography on calmodulin Sepharose. Sepharose 109-118 conjugal transfer protein TraC Escherichia coli 41-45 25371010-0 2016 Culture phases, cytotoxicity and protein expressions of agarose hydrogel induced Sp2/0, A549, MCF-7 cell line 3D cultures. Sepharose 56-63 Sp2 transcription factor Mus musculus 81-86 25371010-2 2016 We demonstrate the usefulness of agarose hydrogels in obtaining 3 dimensional aggregates of three cell lines, A549, MCF-7 and Sp2/0. Sepharose 33-40 Sp2 transcription factor Homo sapiens 126-131 27168827-3 2016 The 27-bp VNTR and G894T polymorphisms of the eNOS gene were genotyped using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism, respectively, followed by agarose gel electrophoresis and DNA sequencing. Sepharose 185-192 nitric oxide synthase 3 Homo sapiens 46-50 27092510-3 2016 When a dye-tethered and prephosphorylated (primed) peptide substrate for GSK3 was employed, a distinct mobility shift in the fluorescent bands on the agarose was observed by GSK3-induced phosphorylation of the primed peptides. Sepharose 150-157 glycogen synthase kinase 3 beta Mus musculus 174-178 26997919-11 2016 The activated OAS enzyme eluted from Sepharose beads showed expression of p46 isoform of OAS1, generally considered the most abundant OAS isoform in elutes from DU14 cell line but not in other prostate cell line. Sepharose 37-46 2'-5'-oligoadenylate synthetase 1 Homo sapiens 89-93 26863527-8 2016 Further, Tpm1.1 binds more strongly than Tpm2.2 to skeletal troponin-Sepharose, as evidenced by a later elution position in the salt gradient. Sepharose 69-78 tropomyosin beta chain Oryctolagus cuniculus 41-45 26934934-0 2016 Soluble Human Intestinal Lactoferrin Receptor: Ca(2+)-Dependent Binding to Sepharose-Based Matrices. Sepharose 75-84 intelectin 1 Homo sapiens 14-45 26826315-0 2016 Fibulin-1 purification from human plasma using affinity chromatography on Factor H-Sepharose. Sepharose 83-92 fibulin 1 Homo sapiens 0-9 26826315-0 2016 Fibulin-1 purification from human plasma using affinity chromatography on Factor H-Sepharose. Sepharose 83-92 complement factor H Homo sapiens 74-82 26668315-7 2016 Analysis of proteins bound to m(7)GTP-Sepharose reveals that both CGP and eIF4G(1357-1600) decrease binding of eIF4E to eIF4G. Sepharose 38-47 eukaryotic translation initiation factor 4 gamma 1 Homo sapiens 74-79 26668315-7 2016 Analysis of proteins bound to m(7)GTP-Sepharose reveals that both CGP and eIF4G(1357-1600) decrease binding of eIF4E to eIF4G. Sepharose 38-47 eukaryotic translation initiation factor 4E Homo sapiens 111-116 26668315-7 2016 Analysis of proteins bound to m(7)GTP-Sepharose reveals that both CGP and eIF4G(1357-1600) decrease binding of eIF4E to eIF4G. Sepharose 38-47 eukaryotic translation initiation factor 4 gamma 1 Homo sapiens 120-125 26647152-10 2016 Additionally, when these samples were applied to the protein A-Sepharose, CT immunoreactivity was retained on the column and was only released after lowering the pH. Sepharose 63-72 calcitonin related polypeptide alpha Homo sapiens 74-76 25777470-2 2016 In this study, trypsin from the pyloric caeca of unicorn leatherjacket was purified by ammonium sulfate precipitation and soybean trypsin inhibitor (SBTI)-Sepharose 4B affinity chromatography. Sepharose 155-164 kunitz trypsin protease inhibitor Glycine max 130-147 26518367-10 2016 Moreover, we purified the NGAL with Ni-Sepharose column. Sepharose 39-48 lipocalin 2 Homo sapiens 26-30 26707401-5 2016 We have also improved a method for the purification of the native isoform by calmodulin-agarose affinity chromatography, and developed a new method to purify the truncated isoform by glutathione-Sepharose affinity chromatography. Sepharose 88-95 calmodulin Saccharomyces cerevisiae S288C 77-87 27433588-5 2016 The complex formation of DPL-PF127 with oligonucleotide was confirmed by running capillary zone electrophoresis (CZE) and agarose gel electrophoresis. Sepharose 122-129 prion like protein doppel Homo sapiens 25-28 26641144-6 2016 The newly formed DNA-peptide probe was then hybridized with miR-21, which was biotinylated and attached to streptavidin agarose in advance. Sepharose 120-127 microRNA 21 Homo sapiens 60-66 26934934-5 2016 Therefore, shLFR (hITLN-1) binds to Sepharose-based matrices in a Ca(2+)-dependent manner. Sepharose 36-45 intelectin 1 Homo sapiens 18-25 25761473-5 2016 Coomassie staining was performed for HP1gamma-binding complexes, using cell lysates prepared by affinity chromatography FLAG-agarose beads, and the bands were digested and then analyzed using a mass spectrometry. Sepharose 125-132 chromobox 3 Homo sapiens 37-45 26505736-6 2016 In the assay established here, where an agarose gel was s.c. implanted in mice and NET formation was detected on the surface of the gel, the extent of the NET formed was inhibited in agarose gels containing rolipram, a PDE4 inhibitor, and butaprost, an EP2 receptor agonist. Sepharose 40-47 prostaglandin E receptor 2 (subtype EP2) Mus musculus 253-265 26505736-6 2016 In the assay established here, where an agarose gel was s.c. implanted in mice and NET formation was detected on the surface of the gel, the extent of the NET formed was inhibited in agarose gels containing rolipram, a PDE4 inhibitor, and butaprost, an EP2 receptor agonist. Sepharose 183-190 prostaglandin E receptor 2 (subtype EP2) Mus musculus 253-265 27826978-6 2016 RESULTS: Our data showed a clear band of ~ 684 bp and ~ 981 bp related to HMGB1 and E7-HMGB1 genes in agarose gel, respectively. Sepharose 102-109 high mobility group box 1 Homo sapiens 74-79 27826978-6 2016 RESULTS: Our data showed a clear band of ~ 684 bp and ~ 981 bp related to HMGB1 and E7-HMGB1 genes in agarose gel, respectively. Sepharose 102-109 high mobility group box 1 Homo sapiens 87-92 25594566-4 2016 In this system, a commercial polyclonal antibody raised against human metal-responsive transcription factor-1 protein (MTF-1 protein) could modify the electrophoretic migration patterns (i.e. cause specific decreases in agarose gel electrophoretic mobility) of the plasmid in the presence or absence of heavy metals other than zinc (Zn). Sepharose 220-227 metal regulatory transcription factor 1 Homo sapiens 119-124 26478185-7 2016 Insulin release from 10% (w/w) implants into agitated solution was faster as compared to release into agarose hydrogel. Sepharose 102-109 insulin Homo sapiens 0-7 26561776-4 2016 PTEN-GST in its reduced and a DTT-reversible H2O2-oxidized form was immobilized on a glutathione-sepharose support and incubated with cell lysate to capture interacting proteins. Sepharose 97-106 phosphatase and tensin homolog Homo sapiens 0-4 27143114-2 2016 For this purpose, hG6PD was initially purified 557-fold at a yield of 51.43% using 2",5"-adenosine diphosphate (ADP) sepharose 4B affinity gel chromatography. Sepharose 117-129 glucose-6-phosphate dehydrogenase Homo sapiens 18-23 26315018-7 2016 We demonstrated co-localization of PEN3 and CaM7, and we confirmed PEN3-CaM interaction in vitro and in vivo by PEN3 pull-down with CaM Sepharose, CaM overlay assay and bimolecular fluorescence complementation. Sepharose 136-145 putative pentacyclic triterpene synthase 3 Arabidopsis thaliana 67-71 26340559-1 2016 In this paper, a new fluorescence bioassay for Golgi protein-73 (GP73), a promising marker for monitoring liver tumor, was developed by using anti-GP73 antibody (GP73 Ab) capped quantum dots (QDs) coupled with protein A/G agarose beads in an attempt to improve the analysis time, cost and operation. Sepharose 222-229 golgi membrane protein 1 Homo sapiens 47-63 26340559-1 2016 In this paper, a new fluorescence bioassay for Golgi protein-73 (GP73), a promising marker for monitoring liver tumor, was developed by using anti-GP73 antibody (GP73 Ab) capped quantum dots (QDs) coupled with protein A/G agarose beads in an attempt to improve the analysis time, cost and operation. Sepharose 222-229 golgi membrane protein 1 Homo sapiens 65-69 26340559-1 2016 In this paper, a new fluorescence bioassay for Golgi protein-73 (GP73), a promising marker for monitoring liver tumor, was developed by using anti-GP73 antibody (GP73 Ab) capped quantum dots (QDs) coupled with protein A/G agarose beads in an attempt to improve the analysis time, cost and operation. Sepharose 222-229 golgi membrane protein 1 Homo sapiens 147-151 26340559-1 2016 In this paper, a new fluorescence bioassay for Golgi protein-73 (GP73), a promising marker for monitoring liver tumor, was developed by using anti-GP73 antibody (GP73 Ab) capped quantum dots (QDs) coupled with protein A/G agarose beads in an attempt to improve the analysis time, cost and operation. Sepharose 222-229 golgi membrane protein 1 Homo sapiens 147-151 26782563-3 2015 The ACE InDel polymorphism was genotyped by polymerase chain reaction (PCR) with specific primers, followed by electrophoresis on 1.5% agarose gel. Sepharose 135-142 angiotensin I converting enzyme Homo sapiens 4-7 26315018-7 2016 We demonstrated co-localization of PEN3 and CaM7, and we confirmed PEN3-CaM interaction in vitro and in vivo by PEN3 pull-down with CaM Sepharose, CaM overlay assay and bimolecular fluorescence complementation. Sepharose 136-145 putative pentacyclic triterpene synthase 3 Arabidopsis thaliana 67-71 26413976-0 2015 Facile synthesis of nano-sized agarose based amino acid-Its pH-dependent protein-like behavior and interactions with bovine serum albumin. Sepharose 31-38 albumin Homo sapiens 124-137 26272754-6 2015 Interaction between heparin and recombinant as well as native full-length neuronal Cav1.2alpha1 channels was confirmed using the heparin-agarose pull down assay. Sepharose 137-144 caveolin 1 Homo sapiens 83-95 26877855-6 2015 SUMO1 antibody was covalently crosslinked to protein A/G agarose. Sepharose 57-64 small ubiquitin-like modifier 1 Rattus norvegicus 0-5 26520021-8 2015 The support l-tyrosine Sepharose used in chromatographic experiments promotes the separation of native pVAX1-LacZ and pcDNA3-FLAG-p53 samples (oc+sc) by decreasing the salt concentration. Sepharose 23-32 tumor protein p53 Homo sapiens 130-133 26544073-7 2015 Co-IP using protein G magnetic Sepharose TM beads suspension was able to capture salivary complexes formed between histatin 5 and its salivary protein partners. Sepharose 31-40 histatin 3 Homo sapiens 115-125 26365067-3 2015 The enzymatic activity and DNA binding ability of glycated RNase A was also investigated by an agarose gel-based assay. Sepharose 95-102 ribonuclease A family member 1, pancreatic Homo sapiens 59-66 26556340-9 2015 We found that sestrin2 siRNA further augmented the formation of 8-OHdG induced by TGI with reperfusion for 4 h. Consistently, sestrin2 siRNA also enhanced apoptosis induced by TGI with reperfusion for 48 h based on the analysis of DNA fragmentation by agarose gel electrophoresis, DNA fragmentation sandwich ELISA, and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay. Sepharose 252-259 sestrin 2 Homo sapiens 14-22 26556340-9 2015 We found that sestrin2 siRNA further augmented the formation of 8-OHdG induced by TGI with reperfusion for 4 h. Consistently, sestrin2 siRNA also enhanced apoptosis induced by TGI with reperfusion for 48 h based on the analysis of DNA fragmentation by agarose gel electrophoresis, DNA fragmentation sandwich ELISA, and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay. Sepharose 252-259 sestrin 2 Homo sapiens 126-134 26192119-3 2015 In this study, we purified a 115 kDa MNSFbeta adduct from murine liver lysates by sequential chromatography on DEAE and anti-MNSFbeta IgG-conjugated Sepharose in the presence of ATP. Sepharose 149-158 Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived) Mus musculus 37-45 26473953-8 2015 Using As-immobilized agarose, we confirmed that As binds directly to hMOF, and that this interaction was competitively inhibited by free As2O3. Sepharose 21-28 lysine acetyltransferase 8 Homo sapiens 69-73 26719671-7 2015 Transfection photographs and agarose gel electrophoresis proved that pDONR223-IFNG could be encased in the albumin nanospheres. Sepharose 29-36 interferon gamma Homo sapiens 78-82 26506342-9 2015 SWE inhibited RSK2 activity, and pull-down assays using SWE-Sepharose beads revealed that SWE binds directly with RSK2 in an ATP-competitive manner. Sepharose 60-69 ribosomal protein S6 kinase A3 Homo sapiens 114-118 26722447-2 2015 METHODS: This study investigated the inhibitory effect of isoliquiritigenin on the growth of U87 glioma cells and its cytotoxicity by MTT method and determined the effect of isoliquiritigenin on TOP I activity by agarose gel electrophoresis. Sepharose 213-220 DNA topoisomerase I Homo sapiens 195-200 26722447-6 2015 Agarose gel electrophoresis showed that isoliquiritigenin had significant inhibitory effect on TOP I activity. Sepharose 0-7 DNA topoisomerase I Homo sapiens 95-100 26118700-8 2015 The Abeta fusion protein was subjected to a Ni-NTA affinity chromatography followed by enterokinase digestion, and the Abeta peptide was purified using glutathione Sepharose affinity chromatography. Sepharose 164-173 amyloid beta precursor protein Homo sapiens 4-9 26118700-8 2015 The Abeta fusion protein was subjected to a Ni-NTA affinity chromatography followed by enterokinase digestion, and the Abeta peptide was purified using glutathione Sepharose affinity chromatography. Sepharose 164-173 amyloid beta precursor protein Homo sapiens 119-124 26192119-3 2015 In this study, we purified a 115 kDa MNSFbeta adduct from murine liver lysates by sequential chromatography on DEAE and anti-MNSFbeta IgG-conjugated Sepharose in the presence of ATP. Sepharose 149-158 Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived) Mus musculus 125-133 26164303-3 2015 The HD-ALB adduct was isolated from HD-exposed plasma with blue Sepharose. Sepharose 64-73 albumin Homo sapiens 7-10 25896960-5 2015 Agarose gel electrophoresis showed a unique bone ALP fraction in BM, which was absent in PB. Sepharose 0-7 alkaline phosphatase, placental Homo sapiens 49-52 26398701-7 2015 A nickel-nitrilotriacetic acid (Ni-NTA) column was first used, and the mPRalpha proteins were then bound to cellulose resin with free amino groups (Cellufine Amino) and finally passed through an SP-Sepharose column. Sepharose 198-207 S100 calcium binding protein A6 (calcyclin) Mus musculus 71-79 25860295-6 2015 Bovine fibrinogen immobilized on CNBr-activated Sepharose 4B beads showed affinity for hemin, Sn-PPIX, Zn-PPIX, and iron-free PPIX in the order Sn-PPIX < iron-free PPIX < hemin < Zn-PPIX. Sepharose 48-60 fibrinogen beta chain Homo sapiens 7-17 26002640-2 2015 In the present study, a novel C1q domain containing protein from Crassostrea gigas (designated CgC1qDC-1) was isolated by liposaccharide-Sepharose 6B affinity chromatography. Sepharose 137-146 complement C1q A chain Homo sapiens 30-33 26091717-9 2015 Monocytes migrating toward agarose beads impregnated with conditioned media from M. tuberculosis-infected monocytes expressed MT1-MMP. Sepharose 27-34 matrix metallopeptidase 14 Homo sapiens 126-133 25998693-2 2015 Here, we determined Tg concentration by isoelectric focusing (IEF) on agarose gel using for detection a rabbit antiserum to human Tg termed FLX. Sepharose 70-77 thyroglobulin Homo sapiens 20-22 25934431-9 2015 A random DNA degradation was observed on agarose gel only in the liver of co-exposed rats to AlCl3 and ACR treatment. Sepharose 41-48 acrosin Rattus norvegicus 103-106 26130925-4 2015 METHODS: The Pax6 transcription factor was purified by heparin agarose affinity chromatography and DEAE cellulose chromatography techniques from the developing zebrafish embryos. Sepharose 63-70 paired box 6a Danio rerio 13-17 25892524-5 2015 Intragenic minisatellite regions in PIR3, HSP150, and DAN4 genes showed high inter-strain size variation detectable by cost-effective agarose gel electrophoresis and were successful to validate six new intra-species S. cerevisiae hybrids from 34 crosses. Sepharose 134-141 beta-1,3-glucan linked protein Saccharomyces cerevisiae S288C 36-40 25892524-5 2015 Intragenic minisatellite regions in PIR3, HSP150, and DAN4 genes showed high inter-strain size variation detectable by cost-effective agarose gel electrophoresis and were successful to validate six new intra-species S. cerevisiae hybrids from 34 crosses. Sepharose 134-141 heat shock protein HSP150 Saccharomyces cerevisiae S288C 42-48 25892524-5 2015 Intragenic minisatellite regions in PIR3, HSP150, and DAN4 genes showed high inter-strain size variation detectable by cost-effective agarose gel electrophoresis and were successful to validate six new intra-species S. cerevisiae hybrids from 34 crosses. Sepharose 134-141 Dan4p Saccharomyces cerevisiae S288C 54-58 25929480-3 2015 The first regime is investigated using agarose gels, which produce a very wide distribution of translocation times for 5 kbp dsDNA fragments, spanning over three orders of magnitude. Sepharose 39-46 kinesin family binding protein Homo sapiens 121-124 25752773-4 2015 On Day 7, PLTs were filtrated through columns with either antibody-coated agarose or rh-megalin bound to antibody-coated agarose. Sepharose 121-128 LDL receptor related protein 2 Homo sapiens 88-95 25752773-5 2015 In addition, we also tested the naked matrix (agarose) and another apheresis surface containing rh-cubilin bound to agarose. Sepharose 116-123 cubilin Homo sapiens 99-106 25908558-0 2015 Agarose gel shift assay reveals that calreticulin favors substrates with a quaternary structure in solution. Sepharose 0-7 calreticulin Homo sapiens 37-49 25343502-3 2015 The conjugation of Apt1 to the surface of liposomes was confirmed by the change in size and zeta potential and by migration on agarose gel electrophoresis. Sepharose 127-134 lysophospholipase 1 Homo sapiens 19-23 26380024-5 2015 The agarose gel electrophoretogram of RT-PCR products further confirmed that UCA1 was increased in NSCLC tissues. Sepharose 4-11 urothelial cancer associated 1 Homo sapiens 77-81 25293592-5 2015 RESULTS: SDS-agarose immunoblotting of patients" plasma revealed the presence of several additional FN-fibrin bands, with decreasing electrophoretic mobilities and increasing molecular masses of 750 kDa, 1000 kDa, 1300 kDa, 1600 kDa and 1900 kDa. Sepharose 13-20 fibronectin 1 Homo sapiens 100-102 26084897-5 2015 DNA from foetal tissue and parental blood samples were extracted, and the genotype analysis of IL-10 -1082G/A promoter polymorphism was carried out by amplification refractory mutation system-polymerase chain reaction followed by agarose gel electrophoresis. Sepharose 230-237 interleukin 10 Homo sapiens 95-100 26146065-4 2015 Under the optimal induction condition, the recombinant NGAL (rNGAL) was expressed and purified by Ni Sepharose 6 Fast Flow affinity chromatography. Sepharose 101-110 lipocalin 2 Homo sapiens 55-59 26146065-4 2015 Under the optimal induction condition, the recombinant NGAL (rNGAL) was expressed and purified by Ni Sepharose 6 Fast Flow affinity chromatography. Sepharose 101-110 lipocalin 2 Rattus norvegicus 61-66 26058017-0 2015 Cytotoxicity of TSP in 3D Agarose Gel Cultured Cell. Sepharose 26-33 thrombospondin 1 Homo sapiens 16-19 26058017-3 2015 The aim of this study was to evaluate the cytotoxicity of TSP on three-dimensionally, agarose gel, cultured cells. Sepharose 86-93 thrombospondin 1 Homo sapiens 58-61 26040922-5 2015 We report a highly sensitive non-radioactive assay that allows the measurement of the activity of purified PTPN2 and of endogenous PTPN2 immunoprecipitated on agarose beads. Sepharose 159-166 protein tyrosine phosphatase non-receptor type 2 Homo sapiens 131-136 25081278-8 2015 The qRT-PCR analysis demonstrated the expression of LEPR mRNA, corresponding the size of the amplified fragment (338 bp), assessed by agarose gel electrophoresis, to that of LEPR mRNA. Sepharose 134-141 leptin receptor Homo sapiens 52-56 25854679-5 2015 Further Zn(2+)-IDA-agarose affinity binding assays showed that Zn(2+) bound to and inhibited PP2Ac(51-270) but not PP2Ac(1-50) or PP2Ac(271-309). Sepharose 19-26 protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform Mus musculus 93-98 24998844-3 2015 Here, the Ras-GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) was identified as a potential target of resveratrol, and in vitro binding assay results using resveratrol-conjugated Sepharose 4B beads confirmed their direct binding. Sepharose 194-206 G3BP stress granule assembly factor 1 Homo sapiens 10-68 26110392-6 2015 Bone-specific osteocalcin content was detected on CaCO3-formed agarose gel on Day 14 of culture, and levels subsequently increased over time. Sepharose 63-70 bone gamma-carboxyglutamate protein Homo sapiens 14-25 26110392-7 2015 Similar osteocalcin content was detected on HA-formed agarose on Day 21 and levels increased on Day 28. Sepharose 54-61 bone gamma-carboxyglutamate protein Homo sapiens 8-19 26110392-8 2015 In contrast, only small amounts of osteocalcin were found on bare agarose gel. Sepharose 66-73 bone gamma-carboxyglutamate protein Homo sapiens 35-46 25346443-8 2015 Cdk5 was expressed in association with Munc18c, p35 and p39, and phosphorylated following human eosinophil activation with eotaxin/CCL11, platelet-activating factor, and secretory IgA-Sepharose. Sepharose 184-193 cyclin dependent kinase 5 Homo sapiens 0-4 26853425-5 2015 The genotyping of stromelysin-1 rs3025058 (-1171, 5A/6A) promoter polymorphism was carried out by amplification refractory mutation system-polymerase chain reaction method followed by agarose gel electrophoresis. Sepharose 184-191 matrix metallopeptidase 3 Homo sapiens 18-31 26012235-6 2015 In msp-1, 16 different alleles were identified by examining size differences in the agarose gels. Sepharose 84-91 merozoite surface protein 1 Plasmodium falciparum 3D7 3-8 25983551-6 2015 VNTR polymorphisms of the DRD4 gene were done by touchdown PCR program using exon 3-specific primers followed by agarose gel electrophoresis. Sepharose 113-120 dopamine receptor D4 Homo sapiens 26-30 25712385-0 2015 Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds. Sepharose 69-76 brain-derived neurotrophic factor Rattus norvegicus 0-33 25712385-1 2015 Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat central nervous system. Sepharose 0-7 brain-derived neurotrophic factor Rattus norvegicus 68-101 25712385-1 2015 Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat central nervous system. Sepharose 0-7 brain-derived neurotrophic factor Rattus norvegicus 103-107 25712385-2 2015 The purpose of this work was to explore whether agarose NGS coated with hydrogen-bonded layer-by-layer (HLbL) could provide an acellular method of delivering prolonged and consistent dosages of active BDNF. Sepharose 48-55 brain-derived neurotrophic factor Rattus norvegicus 201-205 25712385-3 2015 Our results show that HLbL-coated agarose NGS could release BDNF over 10days in consistent dosages averaging 80.5+-12.5(SD)ng/mL. Sepharose 34-41 brain-derived neurotrophic factor Rattus norvegicus 60-64 25677462-2 2015 To simulate the structure of sucrose octasulfate, a natural specific probe for aprotinin, the affinity ligand was composed of an acidic head and a hydrophobic stick, and was then linked with Sepharose. Sepharose 191-200 pancreatic trypsin inhibitor Bos taurus 79-88 25925041-3 2015 METHODS: Talin-1 mRNA and protein expression were examined in NPC cell lines and clinical nasopharyngeal tissues by quantitative RT-PCR, agarose gel electrophoresis and western blotting. Sepharose 137-144 talin 1 Homo sapiens 9-16 25769317-1 2015 A reactive mold-assisted chemical etching (MACE) process through an easy-to-make agarose stamp soaked in bromine methanol etchant to rapidly imprint larger area micro- and nanoarrays on CIGS substrates was demonstrated. Sepharose 81-88 tubulin tyrosine ligase like 5 Homo sapiens 89-94 25769317-2 2015 Interestingly, by using the agarose stamp during the MACE process with and without additive containing oil and triton, CIGS microdome and microhole arrays can be formed on the CIGS substrate. Sepharose 28-35 tubulin tyrosine ligase like 5 Homo sapiens 36-41 25769317-6 2015 Finally, the complete dissolution of agarose stamp into hot water demonstrates an environmentally friendly method by the mold-assisted chemical etching process through an easy-to-make agarose stamp. Sepharose 37-44 tubulin tyrosine ligase like 5 Homo sapiens 45-50 25769317-6 2015 Finally, the complete dissolution of agarose stamp into hot water demonstrates an environmentally friendly method by the mold-assisted chemical etching process through an easy-to-make agarose stamp. Sepharose 37-44 tubulin tyrosine ligase like 5 Homo sapiens 192-197 25769317-6 2015 Finally, the complete dissolution of agarose stamp into hot water demonstrates an environmentally friendly method by the mold-assisted chemical etching process through an easy-to-make agarose stamp. Sepharose 184-191 tubulin tyrosine ligase like 5 Homo sapiens 45-50 25769317-6 2015 Finally, the complete dissolution of agarose stamp into hot water demonstrates an environmentally friendly method by the mold-assisted chemical etching process through an easy-to-make agarose stamp. Sepharose 184-191 tubulin tyrosine ligase like 5 Homo sapiens 192-197 25919299-7 2015 Our data demonstrated that the Ni-NTA agarose affinity-purified A8 scFv inhibited the forward reaction of "on-pathway" aggregation and Abeta fibril maturation. Sepharose 38-45 amyloid beta (A4) precursor protein Mus musculus 135-140 25772225-2 2015 Agarose gel electrophoresis experiments indicated that bifunctional molecules 4b and 5b effectively induced complete plasmid DNA condensation at concentrations up to 40 muM. Sepharose 0-7 latexin Homo sapiens 169-172 25797267-2 2015 Using streptavidin-agarose pulldown and proteomics assay, we identified and validated Ku80, a dimer of Ku participating in the repair of broken DNA double strands, as a new binding protein of the COX-2 gene promoter. Sepharose 19-26 X-ray repair cross complementing 5 Homo sapiens 86-90 25797267-2 2015 Using streptavidin-agarose pulldown and proteomics assay, we identified and validated Ku80, a dimer of Ku participating in the repair of broken DNA double strands, as a new binding protein of the COX-2 gene promoter. Sepharose 19-26 prostaglandin-endoperoxide synthase 2 Homo sapiens 196-201 25899184-8 2015 HLA type was determined by agarose gel electrophoresis and results recorded. Sepharose 27-34 major histocompatibility complex, class II, DR beta 1 Homo sapiens 0-3 25604495-6 2015 In a subgroup of 20 HIV positive and 5 donors, binding of plasma insulin to sIR was determined by ELISA assay of residual insulin levels in plasma immuno-depleted with anti-IR-monoclonal antibody-Sepharose beads. Sepharose 196-205 insulin Homo sapiens 65-72 25974538-7 2015 Agarose gel electrophoresis method was used for determination of both SSB and DSB yields. Sepharose 0-7 small RNA binding exonuclease protection factor La Homo sapiens 70-73 24998844-3 2015 Here, the Ras-GTPase-activating protein SH3 domain-binding protein 1 (G3BP1) was identified as a potential target of resveratrol, and in vitro binding assay results using resveratrol-conjugated Sepharose 4B beads confirmed their direct binding. Sepharose 194-206 G3BP stress granule assembly factor 1 Homo sapiens 70-75 25818779-2 2015 METHODS: The inhibitory effect of anacardic acid on Hsp90 was assessed with in vitro ATPase inhibition assay and ATP-sepharose binding assay. Sepharose 117-126 heat shock protein 90 alpha family class A member 1 Homo sapiens 52-57 25604133-9 2015 These experiments revealed that EGCG-Sepharose bound more HSP90 from metastatic cells compared with NT cells and binding occurred through the HSP90 C-terminus. Sepharose 37-46 heat shock protein 90 alpha family class A member 1 Homo sapiens 58-63 25604133-9 2015 These experiments revealed that EGCG-Sepharose bound more HSP90 from metastatic cells compared with NT cells and binding occurred through the HSP90 C-terminus. Sepharose 37-46 heat shock protein 90 alpha family class A member 1 Homo sapiens 142-147 25462802-5 2015 A strategy has been developed to purify the insoluble MEX67 using a nickel affinity column with chelating Sepharose fast flow media, after solubilizing with sodium lauroyl sarcosinate (Sarkosyl). Sepharose 106-115 Mex67p Saccharomyces cerevisiae S288C 54-59 25602701-4 2015 Analysis of Manduca muscles with 1% SDS-agarose gels and western blots showed two bands near 1 MDa that cross-reacted with antibodies to Drosophila projectin. Sepharose 40-47 bent Drosophila melanogaster 148-157 27047088-8 2015 RESULTS: Bovine IL-15 was amplified and analyzed by agarose gel electrophoresis, which showed a specific product of ~490bp, mature sequence was amplified using full-length as a template to get a product of ~350bp. Sepharose 52-59 interleukin 15 Bos taurus 16-21 25730042-1 2015 The objectives of the present study were to detect an 18-bp deletion mutation in the bovine adenosine monophosphate deaminase 1 (AMPD1) gene and analyze its effect on growth traits in 2 Chinese cattle breeds using DNA sequencing and agarose electrophoresis. Sepharose 233-240 adenosine monophosphate deaminase 1 Bos taurus 92-127 25730042-1 2015 The objectives of the present study were to detect an 18-bp deletion mutation in the bovine adenosine monophosphate deaminase 1 (AMPD1) gene and analyze its effect on growth traits in 2 Chinese cattle breeds using DNA sequencing and agarose electrophoresis. Sepharose 233-240 adenosine monophosphate deaminase 1 Bos taurus 129-134 25450342-1 2015 This paper describes the formation of giant proteoliposomes containing P-glycoprotein (P-gp) from a solution of small proteoliposomes that had been deposited and partially dried on a film of agarose. Sepharose 191-198 ATP binding cassette subfamily B member 1 Homo sapiens 71-85 25444929-4 2015 In MTX-binding assays, HSC70 from L1210/DDP cells showed less affinity for MTX-agarose beads than that of L1210/0 cells. Sepharose 79-86 heat shock protein 8 Mus musculus 23-28 25612818-3 2015 As a result, RecA molecules bound to the ssDNA-SWNTs, as observed using atomic force microscopy and agarose gel electrophoresis. Sepharose 100-107 RAD51 recombinase Homo sapiens 13-17 25463438-3 2015 eIF4E1b exhibited only very weak interactions with m(7)GTP-Sepharose and, rather than binding eIF4G, interacted with 4E-T. Sepharose 59-68 eukaryotic translation initiation factor 4E family member 1B S homeolog Xenopus laevis 0-7 25370824-5 2015 After refolding, Trx/TGF-beta1m fusion was cleaved by enteropeptidase, and the carrier protein of TGF-beta1m was separated from thioredoxin on Ni-NTA agarose. Sepharose 150-157 thioredoxin Homo sapiens 17-20 25450342-1 2015 This paper describes the formation of giant proteoliposomes containing P-glycoprotein (P-gp) from a solution of small proteoliposomes that had been deposited and partially dried on a film of agarose. Sepharose 191-198 ATP binding cassette subfamily B member 1 Homo sapiens 87-91 25463438-8 2015 Indeed, eIF4E1b possesses several distinct features, namely, enhancement of cap binding by a benzyl group at N(7) position of guanine, a reduced response to increasing length of the phosphate chain and increased binding to a cap separated by a linker from Sepharose, suggesting differences in the arrangement of the protein"s core. Sepharose 256-265 eukaryotic translation initiation factor 4E family member 1B S homeolog Xenopus laevis 8-15 24616366-5 2015 Dendritic cells treated with agarose films/OVA induced CD4+CD25+FoxP3+ (T regulatory cells) expression, comparable to untreated iDCs, on autologous T cells in the DC-T co-culture system. Sepharose 29-36 CD4 molecule Homo sapiens 55-58 25448592-7 2015 To generate detergent-free protein, Wnt3a was immobilized on Cu(2+)-charged, iminodiacetic acid-derivatized Sepharose beads, detergent-free buffer was applied and Wnt3a eluted from the beads with buffer containing imidazole plus 30mM methyl-ss-cyclodextrin (MssCD). Sepharose 108-117 wingless-type MMTV integration site family, member 3A Mus musculus 36-41 25615602-0 2015 Effect of natural and semisynthetic pseudoguianolides on the stability of NF-kappaB:DNA complex studied by agarose gel electrophoresis. Sepharose 107-114 nuclear factor kappa B subunit 1 Homo sapiens 74-83 25615602-5 2015 By comparing the relative amount of free DNA fragment to the NF-kappaB - DNA complex, in a routine agarose gel electrophoresis, the destabilizing effect of a compound on the complex is estimated. Sepharose 99-106 nuclear factor kappa B subunit 1 Homo sapiens 61-70 26636139-5 2015 In our present study, a list of potential FXII- and PPK-binding proteins was proposed, using an affinity selection (on agarose-coupled FXII or PPK) from a whole mixture of beta-1,3-glucanase-extrated cell wall-associated proteins and the mass-spectrometry protein identification. Sepharose 119-126 kallikrein B1 Homo sapiens 52-55 25742094-5 2015 Furthermore, pull-down assays with 7-methyl- GTP Sepharose 4B beads indicate that Ligustrazine reduces the available eIF4E for translation initiation. Sepharose 49-61 eukaryotic translation initiation factor 4E Homo sapiens 117-122 25394993-5 2015 FN is PEGylated while it is bound to gelatin Sepharose beads with 2, 5, and 10 kDa PEG precursors. Sepharose 45-54 fibronectin 1 Homo sapiens 0-2 25388665-5 2015 To investigate its interaction with rTMD23, Type I fibroblast growth factor receptor (FGFR1) was precipitated along with syndecan-4 by rTMD23-conjugated Sepharose in human umbilical vein endothelial cells and FGFR1-expressing human embryonic kidney 293 cells. Sepharose 153-162 fibroblast growth factor receptor 1 Homo sapiens 86-91 24616366-5 2015 Dendritic cells treated with agarose films/OVA induced CD4+CD25+FoxP3+ (T regulatory cells) expression, comparable to untreated iDCs, on autologous T cells in the DC-T co-culture system. Sepharose 29-36 interleukin 2 receptor subunit alpha Homo sapiens 59-63 24616366-5 2015 Dendritic cells treated with agarose films/OVA induced CD4+CD25+FoxP3+ (T regulatory cells) expression, comparable to untreated iDCs, on autologous T cells in the DC-T co-culture system. Sepharose 29-36 forkhead box P3 Homo sapiens 64-69 24616366-6 2015 Furthermore, in this co-culture, agarose treatment induced release of IL-12p70 and IL-10 at higher levels as compared with DC treatment with other biomaterial films/OVA, suggesting Th1 and Th2 polarization, respectively. Sepharose 33-40 interleukin 10 Homo sapiens 83-88 24616366-6 2015 Furthermore, in this co-culture, agarose treatment induced release of IL-12p70 and IL-10 at higher levels as compared with DC treatment with other biomaterial films/OVA, suggesting Th1 and Th2 polarization, respectively. Sepharose 33-40 negative elongation factor complex member C/D Homo sapiens 181-184 25428897-5 2015 The recombinant scFv was purified and refolded with Ni-NTA agarose metal affinity column. Sepharose 59-66 immunglobulin heavy chain variable region Homo sapiens 16-20 25617389-0 2015 Resolving Transferrin Isoforms via Agarose Gel Electrophoresis. Sepharose 35-42 transferrin Homo sapiens 10-21 25617389-8 2015 CONCLUSION: Agarose electrophoresis with the MOPS-histidine buffer increases the resolution of transferrin isoforms. Sepharose 12-19 transferrin Homo sapiens 95-106 25176057-6 2015 Kinase and co-precipitation assays with DHGA-D Sepharose 4B beads showed that DHGA-D significantly suppressed MLK3 activity through direct binding to MLK3. Sepharose 47-56 mitogen-activated protein kinase kinase kinase 11 Homo sapiens 110-114 25176057-6 2015 Kinase and co-precipitation assays with DHGA-D Sepharose 4B beads showed that DHGA-D significantly suppressed MLK3 activity through direct binding to MLK3. Sepharose 47-56 mitogen-activated protein kinase kinase kinase 11 Homo sapiens 150-154 25545246-5 2014 Synphilin-1 was pulled down by ATP-agarose beads, and the addition of ATP and ADP reduced this binding, indicating that synphilin-1 bound ADP and ATP. Sepharose 35-42 synuclein alpha interacting protein Homo sapiens 0-11 26163783-8 2015 Agarose gel electrophoresis experiment with plasmid pBR 322 reveals that the groove binded ADMQ result in a concentration dependent cleavage of plasmid DNA into its supercoiled and nicked circular form. Sepharose 0-7 translocator protein Bos taurus 52-55 25545246-5 2014 Synphilin-1 was pulled down by ATP-agarose beads, and the addition of ATP and ADP reduced this binding, indicating that synphilin-1 bound ADP and ATP. Sepharose 35-42 synuclein alpha interacting protein Homo sapiens 120-131 25374779-1 2014 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the skeletal muscle of euthermic and torpid Ictidomys tridecemlineatus was purified to electrophoretic homogeneity using a novel method involving Blue-agarose and Phenyl-agarose chromatography. Sepharose 205-212 glyceraldehyde-3-phosphate dehydrogenase Ictidomys tridecemlineatus 0-40 25465013-1 2014 We have previously studied poly(ethylenimine) (PEI)-grafted Sepharose FF resins for ion-exchange chromatography of bovine serum albumin (BSA), and found the presence of a critical ionic capacity (cIC, 600mmol/L for BSA), above which both BSA adsorption capacity and uptake rates increased drastically. Sepharose 60-69 albumin Homo sapiens 122-135 25195176-3 2014 The recombinant NDPK (rNDPK) was overexpressed in Escherichia coli and purified to homogeneity by Ni-NTA agarose affinity chromatography, HiTrap SP HP cation exchange chromatography and HiLoad 16/60 Superdex 200 gel filtration chromatography. Sepharose 105-112 abnormal wing discs Drosophila melanogaster 16-20 24992915-5 2014 Agarose gel electrophoresis experiment shows that PBR 322 DNA can be induced to cleave by two compounds under photoactivated condition. Sepharose 0-7 translocator protein Homo sapiens 50-53 25426414-4 2014 In this study, using lysophosphatidic acid (LPA)-coated agarose beads, we have identified FABP3 as an LPA carrier protein in human coronary artery endothelial cells (HCAECs). Sepharose 56-63 fatty acid binding protein 3 Homo sapiens 90-95 32262115-7 2014 Agarose gel electrophoresis demonstrated that pOA-EGFP (containing the osteoactivin and the green fluorescent protein fusion gene) can be completely absorbed and protected from DNase I degradation by the aminated rMBGs. Sepharose 0-7 glycoprotein nmb Homo sapiens 71-83 25374779-1 2014 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the skeletal muscle of euthermic and torpid Ictidomys tridecemlineatus was purified to electrophoretic homogeneity using a novel method involving Blue-agarose and Phenyl-agarose chromatography. Sepharose 205-212 glyceraldehyde-3-phosphate dehydrogenase Ictidomys tridecemlineatus 42-47 25211019-1 2014 We demonstrate successful incorporation of the G protein coupled receptor 5-HT1A into giant unilamellar vesicles using an agarose rehydration method. Sepharose 122-129 5-hydroxytryptamine receptor 1A Homo sapiens 74-80 25358000-6 2014 To obtain a recombinant plasmid, first a plasmid carrying an FVIII gene fragment (pCMV6-hFVIII) was digested by EcoRI-SalI restriction enzymes and then the fragment was purified from agarose gel and inserted into a pUCWAP7 vector carrying a tissue-specific promoter (mWAP 4.1 kbp). Sepharose 183-190 coagulation factor VIII Mus musculus 61-66 24974776-2 2014 When single-stranded DNA (ssDNA) was used for the hybridization, significant binding of the SSB molecules to the ssDNA-SWNT hybrids was observed by using atomic force microscopy (AFM) and agarose gel electrophoresis. Sepharose 188-195 small RNA binding exonuclease protection factor La Homo sapiens 92-95 25205747-8 2014 Superoxide dismutase, catalase and glutathione peroxidase were significantly enhanced in agarose-chitosan-treated group compared with that of control group. Sepharose 89-96 catalase Oryctolagus cuniculus 22-30 25232407-5 2014 RT-PCR and agarose gel electrophoresis were used to confirm the expression of PHD3 in HepG2 cells and transfection successfully. Sepharose 11-18 egl-9 family hypoxia inducible factor 3 Homo sapiens 78-82 25127031-6 2014 Since HMGB1 binds to DNA with high affinity, here we developed a novel strategy to sequester HMGB1 using DNA immobilized on sepharose beads. Sepharose 124-133 high mobility group box 1 Mus musculus 6-11 25127031-6 2014 Since HMGB1 binds to DNA with high affinity, here we developed a novel strategy to sequester HMGB1 using DNA immobilized on sepharose beads. Sepharose 124-133 high mobility group box 1 Mus musculus 93-98 25179631-5 2014 This domain, coupled to nickel nitrilotriacetic acid (Ni-NTA) agarose beads, specifically recruited F-actin in the presence of CFL-1 and, when expressed in HeLa cells, inhibited Ca(2+) entry into the TGN and secretory cargo sorting. Sepharose 62-69 cofilin 1 Homo sapiens 127-132 24862437-0 2014 Selective purification of supercoiled p53-encoding pDNA with L-methionine-agarose matrix. Sepharose 74-81 tumor protein p53 Homo sapiens 38-41 24984153-7 2014 One of the selected compounds, 50F10, was directly shown to preserve the active homodimer structure of LPL, as demonstrated by heparin-Sepharose chromatography. Sepharose 135-144 lipoprotein lipase Homo sapiens 103-106 25423754-3 2014 The fusion protein TAT-Cygb, whose expression was induced by lactose, was purified by CM Sepharose Fast Flow Protocol and verified by Western blotting. Sepharose 89-98 cytoglobin Homo sapiens 23-27 24704550-8 2014 In the cell-free assay, we compared the binding of internally tagged WT-LPL and S447X-LPL to soluble GPIHBP1 immobilized on agarose beads. Sepharose 124-131 lipoprotein lipase Homo sapiens 86-89 24982360-3 2014 Interleukin-6 polymorphism was detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and analyzed in 3% agarose gel. Sepharose 140-147 interleukin 6 Homo sapiens 0-13 24831556-3 2014 MAIN METHODS: Using an immunoprecipitation (IP) kit, at first the antibody of G-CSF was covalently crosslinked to protein A/G agarose. Sepharose 126-133 colony stimulating factor 3 Rattus norvegicus 78-83 24831556-4 2014 Then the mouse brain or PC12 cell lysate mixed with G-CSF was added to the agarose beads plus antibody. Sepharose 75-82 colony stimulating factor 3 Rattus norvegicus 52-57 24704550-8 2014 In the cell-free assay, we compared the binding of internally tagged WT-LPL and S447X-LPL to soluble GPIHBP1 immobilized on agarose beads. Sepharose 124-131 glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1 Homo sapiens 101-108 25108334-2 2014 Results obtained by retardation of RNP complexes in agarose gel were confirmed by Western-Northern hybridization. Sepharose 52-59 33 kDa ribonucleoprotein, chloroplastic-like Nicotiana tabacum 35-38 25909061-4 2014 A 45 bp I/D polymorphism in hUCP2 gene was genotyped by polymerase chain reaction (PCR) amplification and agarose gel electrophoresis method. Sepharose 106-113 uncoupling protein 2 Homo sapiens 28-33 24522836-6 2014 The established tool for selective inline quantification was successfully applied for a peak deconvolution of a co-eluting ternary protein mixture consisting of lysozyme, ribonuclease A, and cytochrome c on SP Sepharose FF. Sepharose 210-219 cytochrome c, somatic Homo sapiens 191-203 24755219-5 2014 By adding streptavidin-Sepharose beads to GL-biot-treated DU145 cell lysates, STAT3 was isolated and identified as a target protein. Sepharose 23-32 signal transducer and activator of transcription 3 Homo sapiens 78-83 24239005-2 2014 To investigate the long-term stability of tissue engineered cartilage constructs, we assessed the development of compressive mechanical properties of chondrocyte and mesenchymal stem cell (MSC)-laden three dimensional agarose constructs cultured in a well defined chondrogenic in vitro environment through 112 days. Sepharose 218-225 musculin Homo sapiens 189-192 24915096-2 2014 The catalytic domain of mouse Enpp6 was expressed in HEK293T cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. Sepharose 104-113 ectonucleotide pyrophosphatase/phosphodiesterase 6 Mus musculus 30-35 24206165-4 2014 The extracellular domain of RAGE was immobilized onto agarose beads to generate the bioadsorbent. Sepharose 54-61 long intergenic non-protein coding RNA 914 Homo sapiens 28-32 24376134-3 2014 Here, we describe a novel affinity process with the dye Yellow HE-4R immobilized on Sepharose for bovine lactoferrin purification. Sepharose 84-93 lactotransferrin Bos taurus 105-116 24751557-4 2014 We observed that the number of functional adsorption sites was smaller at high ionic strength and that these sites had reduced desorption kinetic heterogeneity, and thus narrower predicted elution profiles, for the anion-exchange adsorption of alpha-lactalbumin on an agarose-supported, clustered-charge ligand stationary phase. Sepharose 268-275 lactalbumin alpha Homo sapiens 244-261 24885534-2 2014 METHODS: Transcripts of FAK expressed in 102 human breast tumor tissues and 52 corresponding normal tissues were analyzed by RT-PCR and DNA sequencing, as well as agarose gel electrophoresis. Sepharose 163-170 protein tyrosine kinase 2 Homo sapiens 24-27 24940539-7 2014 Furthermore, the TPEF image quality of 1mum fluorescent beads sealed in agarose gel at different depths is improved. Sepharose 72-79 transmembrane protein with EGF like and two follistatin like domains 2 Homo sapiens 17-21 24652286-6 2014 Using an agarose overlay to trap the HA-containing matrix, the HC-HA-PTX3 complex was formed, as analyzed by Western blot analysis, by AM cells but not human skin fibroblasts, despite being cultured in the presence of serum and TNF. Sepharose 9-16 pentraxin 3 Homo sapiens 69-73 24652286-7 2014 However, exogenous PTX3 helps human skin fibroblasts form the HC-HA-PTX3 complex with an agarose overlay. Sepharose 89-96 pentraxin 3 Homo sapiens 19-23 24652286-7 2014 However, exogenous PTX3 helps human skin fibroblasts form the HC-HA-PTX3 complex with an agarose overlay. Sepharose 89-96 pentraxin 3 Homo sapiens 68-72 24652286-8 2014 Furthermore, PTX3 can be coimmunoprecipitated with the HC-HA complex from agarose-overlaid AM cell extracts by an anti-human IalphaI antibody. Sepharose 74-81 pentraxin 3 Homo sapiens 13-17 24765192-6 2014 The detection of p33ING1b methylation status by incubation of DNA contained in agarose beads for bisulfite modification, followed by nMSP, is a promising non-invasive screening method for CRCs and precancerous lesions. Sepharose 79-86 inhibitor of growth family member 1 Homo sapiens 17-25 23650117-3 2014 Although both peptide and agarose hydrogels retained TGF-beta1, fivefold higher retention was found in peptide. Sepharose 26-33 transforming growth factor beta 1 Equus caballus 53-62 24671039-5 2014 The EP18 oligonucleotide which binds to the CAAT enhancer binding protein (C/EBP) was synthesized with a 3" ribose (rEP18) and coupled to hydrazide-agarose. Sepharose 148-155 CCAAT enhancer binding protein alpha Homo sapiens 44-73 24671039-5 2014 The EP18 oligonucleotide which binds to the CAAT enhancer binding protein (C/EBP) was synthesized with a 3" ribose (rEP18) and coupled to hydrazide-agarose. Sepharose 148-155 CCAAT enhancer binding protein alpha Homo sapiens 75-80 24582080-0 2014 In situ silver nanoparticles synthesis in agarose film supported on filter paper and its application as highly efficient SERS test stripes. Sepharose 42-49 seryl-tRNA synthetase 2, mitochondrial Homo sapiens 121-125 24582080-1 2014 Highly efficient multifunctional agarose SERS test stripes has been fabricated by a convenient facile approach. Sepharose 33-40 seryl-tRNA synthetase 2, mitochondrial Homo sapiens 41-45 24616222-8 2014 Impairment of this PI3-kinase pathway results in slow movement under agarose and cells that produce few blebs, though actin polymerization appears unaffected. Sepharose 69-76 peptidase inhibitor 3 Homo sapiens 19-22 23941573-5 2014 Recombinant hBMP4 protein was purified by SP Sepharose and heparin affinity chromatography. Sepharose 45-54 bone morphogenetic protein 4 Homo sapiens 12-17 24637843-7 2014 After incubating infected E. coli on agarose that contains the correct substrate, plaques can be scored; blue plaques indicate a mutant LacI gene, while clear plaques harbor wild-type. Sepharose 37-44 tissue factor pathway inhibitor Mus musculus 136-140 24558465-8 2014 A pull-down assay using CCG-1423 Sepharose revealed the direct binding of CCG-1423 to MRTF-A. Sepharose 33-42 myocardin related transcription factor A Homo sapiens 86-92 24558465-10 2014 G-actin did not bind to CCG-1423 Sepharose, but the interaction between MRTF-A and CCG-1423 Sepharose was reduced in the presence of G-actin. Sepharose 92-101 myocardin related transcription factor A Homo sapiens 72-78 24463400-1 2014 Single-domain antibody generation technology was applied to make new Sepharose-bound ligands for affinity separation of closely related proteins, such as human and goat lactoferrin. Sepharose 69-78 lactotransferrin Capra hircus 169-180 24337186-0 2014 An alternative easy method for antibody purification and analysis of protein-protein interaction using GST fusion proteins immobilized onto glutathione-agarose. Sepharose 152-159 glutathione S-transferase kappa 1 Homo sapiens 103-106 24474754-5 2014 We first confirmed the presence of physically induced, TRPV4-dependent intracellular Ca(2+) signaling in agarose-embedded chondrocytes, and then used this model system to study the role of TRPV4 in regulating the response of chondrocytes to dynamic compression. Sepharose 105-112 transient receptor potential cation channel subfamily V member 4 Homo sapiens 55-60 24551446-7 2014 Furthermore, upregulation of matrix metalloproteinase 9 and matrix metalloproteinase 2 activity and increased expression of vascular endothelial growth factor-A and hypoxia-inducible factor-1alpha were shown in agarose-engineered tumors. Sepharose 211-218 matrix metallopeptidase 9 Homo sapiens 29-55 24551446-7 2014 Furthermore, upregulation of matrix metalloproteinase 9 and matrix metalloproteinase 2 activity and increased expression of vascular endothelial growth factor-A and hypoxia-inducible factor-1alpha were shown in agarose-engineered tumors. Sepharose 211-218 matrix metallopeptidase 2 Homo sapiens 60-86 24551446-7 2014 Furthermore, upregulation of matrix metalloproteinase 9 and matrix metalloproteinase 2 activity and increased expression of vascular endothelial growth factor-A and hypoxia-inducible factor-1alpha were shown in agarose-engineered tumors. Sepharose 211-218 vascular endothelial growth factor A Homo sapiens 124-160 24647109-7 2014 Solubilized His-tagged scFv proteins were purified using Ni(2+)-Sepharose column chromatography in the presence of 3.5 M Gdn-HCl. Sepharose 64-73 immunglobulin heavy chain variable region Homo sapiens 23-27 24337186-5 2014 The protocol for immobilization of GST-SPINK3 to glutathione-agarose beads was modified from previously reported protocols by using an alternative bifunctional cross-linker (dithiobis(succinimidyl propionate)) in a very simple procedure and by using simple buffers under physiological conditions. Sepharose 61-68 glutathione S-transferase kappa 1 Homo sapiens 35-38 24337186-5 2014 The protocol for immobilization of GST-SPINK3 to glutathione-agarose beads was modified from previously reported protocols by using an alternative bifunctional cross-linker (dithiobis(succinimidyl propionate)) in a very simple procedure and by using simple buffers under physiological conditions. Sepharose 61-68 serine peptidase inhibitor Kazal type 1 Homo sapiens 39-45 24078189-0 2014 Quantitative determination of glycine in aqueous solution using glutamate dehydrogenase-immobilized glyoxal agarose beads. Sepharose 108-115 glutamate dehydrogenase 1 Homo sapiens 64-87 24078189-1 2014 In this study, an enzymatic procedure for the determination of glycine (Gly) was developed by using a column containing immobilized glutamate dehydrogenase (GDH) on glyoxal agarose beads. Sepharose 173-180 glutamate dehydrogenase 1 Homo sapiens 132-155 24078189-1 2014 In this study, an enzymatic procedure for the determination of glycine (Gly) was developed by using a column containing immobilized glutamate dehydrogenase (GDH) on glyoxal agarose beads. Sepharose 173-180 glutamate dehydrogenase 1 Homo sapiens 157-160 24078189-6 2014 The interaction between GDH and glyoxal agarose beads was analyzed by Fourier transform infrared (FTIR) spectroscopy. Sepharose 40-47 glutamate dehydrogenase 1 Homo sapiens 24-27 25374234-3 2014 HSP70 was purified from renal cell carcinoma specimens by serial column chromatography on Con A-sepharose, PD-10, ADP-agarose and DEAE-cellulose, and finally subjected to fast protein liquid chromatography (FPLC). Sepharose 96-105 heat shock protein family A (Hsp70) member 4 Homo sapiens 0-5 25374234-3 2014 HSP70 was purified from renal cell carcinoma specimens by serial column chromatography on Con A-sepharose, PD-10, ADP-agarose and DEAE-cellulose, and finally subjected to fast protein liquid chromatography (FPLC). Sepharose 118-125 heat shock protein family A (Hsp70) member 4 Homo sapiens 0-5 24877104-7 2014 DAPK promoter hypermethylation was carried out using bisulfite-modified genomic DNA in methylation-specific PCR followed by separation in agarose gels. Sepharose 138-145 death associated protein kinase 1 Homo sapiens 0-4 25134395-1 2014 BACKGROUND: Although the method of choice to detect M-protein is electrophoresis on an agarose gel, such gel electrophoresis (GE) is labor-intensive, time-consuming, and not standardized. Sepharose 87-94 myomesin 2 Homo sapiens 52-61 24154594-8 2014 The antibody prepared in rabbit against P23 was bound to Sepharose 4B and used for the isolation of oocysts. Sepharose 57-66 tumor protein, translationally-controlled 1 Gallus gallus 40-43 24367651-3 2013 We quantitatively and qualitatively compared the expression of titin isoforms between Rbm20(-/-) and wild type rats by real time RT-PCR and SDS agarose electrophoresis. Sepharose 144-151 titin Rattus norvegicus 63-68 32261331-5 2013 The same photoluminescence quenching was also observed in gel form, when a GOx modified CdTe QDs loaded agarose gel was dipped in H2O2 and glucose solutions, respectively. Sepharose 104-111 hydroxyacid oxidase 1 Homo sapiens 75-78 24358207-10 2013 The specific binding of Sp1 to R3 region of LDLR 5"-untranslated region was demonstrated with electrophoretic mobility shift assay (EMSA) and streptavidin-agarose precipitation assay followed by western blotting. Sepharose 155-162 low density lipoprotein receptor Homo sapiens 44-48 24548388-2 2013 METHODS: FMR1 CGG repeats were analyzed in 157 MSA patients by polymerase chain reaction, agarose gel electrophoresis and capillary electrophoresis. Sepharose 90-97 fragile X messenger ribonucleoprotein 1 Homo sapiens 9-13 23904295-4 2013 The virulence factor Int1 contained the most putative heparin-binding motifs (n = 5); peptides encompassing 2 of 5 motifs bound to heparin-Sepharose. Sepharose 139-148 Wnt family member 1 Rattus norvegicus 21-25 24025409-0 2013 Treatment of agarose-agarose RENCA macrobeads with docetaxel selects for OCT4(+) cells with tumor-initiating capability. Sepharose 13-20 POU domain, class 5, transcription factor 1, related sequence 1 Mus musculus 73-77 24025409-0 2013 Treatment of agarose-agarose RENCA macrobeads with docetaxel selects for OCT4(+) cells with tumor-initiating capability. Sepharose 21-28 POU domain, class 5, transcription factor 1, related sequence 1 Mus musculus 73-77 23892357-5 2013 In addition, only biotin-labeled CAPE analogues with the catechol moiety precipitated Kelch-like ECH associated protein 1 (Keap1) when incubated with cell lysates and streptavidin agarose beads. Sepharose 180-187 structural maintenance of chromosomes 2 Rattus norvegicus 33-37 24055435-6 2013 Here we describe the development of an IEMR using His6-tagged TK and TAm immobilized onto Ni-NTA agarose beads and packed into tubes to enable multi-step enzyme reactions. Sepharose 97-104 transketolase Homo sapiens 62-64 24076026-5 2013 Agarose gel electrophoresis showed that the nuclease activity of AtCaN2 against lambdaDNA was inhibited by AtCYSb, which suggests that AtCYSb regulates nucleic acid degradation in cells. Sepharose 0-7 Ca(2+)-dependent nuclease family protein Arabidopsis thaliana 65-71 24244337-5 2013 The Protein A-AMCase-V5-His was purified from periplasmic fractions using an IgG Sepharose column followed by a Ni Sepharose chromatography. Sepharose 81-90 chitinase, acidic 1 Mus musculus 14-20 24244337-5 2013 The Protein A-AMCase-V5-His was purified from periplasmic fractions using an IgG Sepharose column followed by a Ni Sepharose chromatography. Sepharose 115-124 chitinase, acidic 1 Mus musculus 14-20 24180592-5 2013 m7GTP-Sepharose-binding assay revealed that Src activity is required to form eIF4F complex which is necessary for Cap-dependent translation in alpha6beta4 expressing human cancer cells. Sepharose 6-15 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 44-47 24076026-5 2013 Agarose gel electrophoresis showed that the nuclease activity of AtCaN2 against lambdaDNA was inhibited by AtCYSb, which suggests that AtCYSb regulates nucleic acid degradation in cells. Sepharose 0-7 cystatin B Arabidopsis thaliana 107-113 24076026-5 2013 Agarose gel electrophoresis showed that the nuclease activity of AtCaN2 against lambdaDNA was inhibited by AtCYSb, which suggests that AtCYSb regulates nucleic acid degradation in cells. Sepharose 0-7 cystatin B Arabidopsis thaliana 135-141 24129228-6 2013 The mRNA expression of ET-1 was analyzed by RT-PCR and agarose gel. Sepharose 55-62 endothelin 1 Mus musculus 23-27 23987386-2 2013 NA-agarose was characterized by TGA, GPC, UV spectrophotometry, fluorescence spectroscopy, FT-IR, (1)H and (13)C NMR spectra, exhibiting that in NA-agarose the naphthylacetyl group was attached to the backbone of the agarose polymer. Sepharose 3-10 T-box transcription factor 1 Homo sapiens 32-35 23987386-2 2013 NA-agarose was characterized by TGA, GPC, UV spectrophotometry, fluorescence spectroscopy, FT-IR, (1)H and (13)C NMR spectra, exhibiting that in NA-agarose the naphthylacetyl group was attached to the backbone of the agarose polymer. Sepharose 3-10 glycophorin C (Gerbich blood group) Homo sapiens 37-40 32261103-3 2013 The performances of the EA-G2 (or EA-G1) were assayed by enzyme degradation, MTT method and agarose gel electrophoresis. Sepharose 92-99 potassium voltage-gated channel subfamily H member 5 Homo sapiens 24-29 32261103-3 2013 The performances of the EA-G2 (or EA-G1) were assayed by enzyme degradation, MTT method and agarose gel electrophoresis. Sepharose 92-99 potassium voltage-gated channel subfamily H member 1 Homo sapiens 34-39 23864430-2 2013 In this study, human fibrinogen was immobilized on CNBr-activated Sepharose 4B beads. Sepharose 66-78 fibrinogen beta chain Homo sapiens 21-31 23864430-3 2013 The fibrinogen beads bound hemin (iron-protoporphyrin IX: PPIX) as well as iron ion released from ferrous ammonium sulfate (FAS) more efficiently than Sepharose 4B beads alone. Sepharose 151-163 fibrinogen beta chain Homo sapiens 4-14 23892544-5 2013 Biologically active CCL25 was coupled to a Sepharose matrix and demonstrated to selectively remove CCR9-expressing cells leaving other cell populations largely unaffected. Sepharose 43-52 C-C motif chemokine ligand 25 Homo sapiens 20-25 23892544-5 2013 Biologically active CCL25 was coupled to a Sepharose matrix and demonstrated to selectively remove CCR9-expressing cells leaving other cell populations largely unaffected. Sepharose 43-52 C-C motif chemokine receptor 9 Homo sapiens 99-103 23925886-6 2013 Passive protection of mice against pneumococci and killing in the modified OPKA were lost when normal human sera were adsorbed with recombinant PspA (rPspA) on Sepharose, thus supporting the potential utility of the modified OPKA to detect protective antibodies to PspA. Sepharose 160-169 surfactant protein A1 Homo sapiens 144-148 23925886-6 2013 Passive protection of mice against pneumococci and killing in the modified OPKA were lost when normal human sera were adsorbed with recombinant PspA (rPspA) on Sepharose, thus supporting the potential utility of the modified OPKA to detect protective antibodies to PspA. Sepharose 160-169 surfactant protein A1 Homo sapiens 151-155 24497714-3 2013 TaqI VDR gene polymorphism was analyzed using specific primers and amplified by polymerase chain reaction (PCR) and visualized under 2% agarose gel. Sepharose 136-143 vitamin D receptor Homo sapiens 5-8 22803665-2 2013 GST was purified 3089 fold with a specific activity of 20 U/mg and a yield of 78% from gastric tumour tissue; and 1185 fold with a specific activity of 5.69 U/mg and a yield of 50% from nontumour tissue by using glutathione-agarose affinity column, respectively. Sepharose 224-231 glutathione S-transferase kappa 1 Homo sapiens 0-3 23806217-8 2013 PKA was cosedimented with nNOS by ADP agarose gel. Sepharose 38-45 nitric oxide synthase 1 Rattus norvegicus 26-30 23906520-2 2013 CFH was isolated from human plasma by polyethylene glycol (PEG) precipitation, following three sequential chromatographic columns, which consisted of l-lysine Sepharose column, Resource Q column and Sephacryl S-300 High Resolution HiPrep 16/60 column. Sepharose 159-168 complement factor H Homo sapiens 0-3 24645321-9 2013 TAE agarose gel electrophoresis was used to confirm SSB protein-RNA binding activity. Sepharose 4-11 small RNA binding exonuclease protection factor La Homo sapiens 52-55 24510786-2 2013 First, the plasma is depleted of fibronectin plus other heparin- and Sepharose-binding proteins and treated with urea to activate the heparin-binding activity of vitronectin, which is subsequently bound to a heparin affinity column and eluted. Sepharose 69-78 vitronectin Homo sapiens 162-173 23885665-4 2013 After cell disruption, His-tagged GP5 protein was successfully purified by Ni(2+)-chelating Sepharose Fast Flow with a yield and purity of 80.5% and 48%, respectively. Sepharose 92-101 glycoprotein V platelet Homo sapiens 34-37 23320930-7 2013 On the other hand, the relative gene expressions of E-cadherin and IL-1beta in epithelial cells of the 3D culture under decidualization conditions significantly differed from those in epithelial cells grown over the fibrin-agarose gel matrix without stromal cells, pointing to regulation of epithelial cells by the stroma. Sepharose 223-230 cadherin 1 Homo sapiens 52-62 23320930-7 2013 On the other hand, the relative gene expressions of E-cadherin and IL-1beta in epithelial cells of the 3D culture under decidualization conditions significantly differed from those in epithelial cells grown over the fibrin-agarose gel matrix without stromal cells, pointing to regulation of epithelial cells by the stroma. Sepharose 223-230 interleukin 1 beta Homo sapiens 67-75 23928160-5 2013 Fluorescence responses to glucose were preserved when GBP-Blue Oxazine was immobilised to agarose beads, and the beads were excited by NIR light through a mouse skin preparation studied in vitro. Sepharose 90-97 lectin, galactose binding, soluble 3 Mus musculus 54-57 23882697-4 2013 MUC5AC mRNA transcript was amplified by a customized RT-PCR assay and detected in PCR strips based on reverse dot blot hybridization and in agarose gels. Sepharose 140-147 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 0-6 23188093-15 2013 Furthermore, agarotetraose-mediated HO-1 induction required NF-E2-related factor 2 function and phosphorylation of c-jun N-terminal kinase. Sepharose 13-26 heme oxygenase 1 Mus musculus 36-40 23958855-12 2013 Integrin subunits alphav, beta1 and beta6, but not alpha2, bound to TNC immobilized on agarose beads in a divalent cation-dependent manner. Sepharose 87-94 potassium calcium-activated channel subfamily M regulatory beta subunit 1 Homo sapiens 26-31 23958855-12 2013 Integrin subunits alphav, beta1 and beta6, but not alpha2, bound to TNC immobilized on agarose beads in a divalent cation-dependent manner. Sepharose 87-94 tenascin C Homo sapiens 68-71 23833064-5 2013 Cdc42 activation was determined by pull-down assays with PAK-agarose beads. Sepharose 61-68 cell division control protein 42 homolog Oryctolagus cuniculus 0-5 23188093-15 2013 Furthermore, agarotetraose-mediated HO-1 induction required NF-E2-related factor 2 function and phosphorylation of c-jun N-terminal kinase. Sepharose 13-26 nuclear factor, erythroid derived 2, like 2 Mus musculus 60-82 24369620-2 2013 First, the thrombin affinity chromatography media was prepared through thrombin coupled with cyanogen bromide activated agarose. Sepharose 120-127 coagulation factor II, thrombin Homo sapiens 11-19 24369620-5 2013 The optimum conditions were as follows: the use of Na2CO3-NaHCO3 (containing 0.5 mol/L NaCl) solution of pH 8.3 as the reaction solution, 200 U thrombin per 1 g agarose, and reaction for 10 h at room temperature. Sepharose 161-168 coagulation factor II, thrombin Homo sapiens 144-152 23311625-11 2013 The analysis of the chondrocyte-agarose constructs by using real-time polymerase chain reaction, Western-blotting, immunohistochemistry, and electron microscopy techniques demonstrated that the BMP-2/Col1a1 siRNA combination is effective in reinitializing correct production and assembly of the cartilage-characteristic matrix in agarose hydrogel, without production of type I collagen. Sepharose 32-39 bone morphogenetic protein 2 Mus musculus 194-199 23311625-11 2013 The analysis of the chondrocyte-agarose constructs by using real-time polymerase chain reaction, Western-blotting, immunohistochemistry, and electron microscopy techniques demonstrated that the BMP-2/Col1a1 siRNA combination is effective in reinitializing correct production and assembly of the cartilage-characteristic matrix in agarose hydrogel, without production of type I collagen. Sepharose 32-39 collagen, type I, alpha 1 Mus musculus 200-206 23311625-11 2013 The analysis of the chondrocyte-agarose constructs by using real-time polymerase chain reaction, Western-blotting, immunohistochemistry, and electron microscopy techniques demonstrated that the BMP-2/Col1a1 siRNA combination is effective in reinitializing correct production and assembly of the cartilage-characteristic matrix in agarose hydrogel, without production of type I collagen. Sepharose 330-337 bone morphogenetic protein 2 Mus musculus 194-199 23311625-11 2013 The analysis of the chondrocyte-agarose constructs by using real-time polymerase chain reaction, Western-blotting, immunohistochemistry, and electron microscopy techniques demonstrated that the BMP-2/Col1a1 siRNA combination is effective in reinitializing correct production and assembly of the cartilage-characteristic matrix in agarose hydrogel, without production of type I collagen. Sepharose 330-337 collagen, type I, alpha 1 Mus musculus 200-206 23542127-3 2013 By employing agarose gel electrophoresis, a fluorescent dye, PicoGreen, intercalation into mtDNA, and long-range PCR (LR-PCR), we showed that mitochondrial DNA conformational stability is related to HtrA2. Sepharose 13-20 HtrA serine peptidase 2 Mus musculus 199-204 23692254-3 2013 Here, we identified a novel bisanilino pyrimidine, CTx-0294885, exhibiting inhibitory activity against a broad range of kinases in vitro, and further developed it into a Sepharose-supported kinase capture reagent. Sepharose 170-179 cytochrome P450 family 27 subfamily A member 1 Homo sapiens 51-54 23754290-4 2013 In this study, we have identified porcine plasma prothrombin as SSL10-binding protein by affinity purification using SSL10-conjugated Sepharose. Sepharose 134-143 coagulation factor II Mus musculus 49-60 23861745-6 2013 From agarose gel retardation assay (AGRA), Ni-NTA pull-down and atomic force microscopy (AFM) studies we show that amino acids 105-183 of INI1 comprise the minimal DNA binding domain (DBD). Sepharose 5-12 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 Homo sapiens 138-142 32481828-2 2013 Agarose hydrogels were first seeded with FPSCs at different seeding densities and maintained in a chondrogenic media supplemented with TGF-beta3. Sepharose 0-7 transforming growth factor beta 3 Homo sapiens 135-144 22628165-5 2013 DPD was synthesized and characterized by agarose gel electrophoresis, particle size and zeta potential. Sepharose 41-48 dihydropyrimidine dehydrogenase Homo sapiens 0-3 23828749-8 2013 TNF-alpha genotyping at position-308 (G A) was carried out by amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) method followed by agarose gel electrophoresis. Sepharose 161-168 tumor necrosis factor Homo sapiens 0-9 23648095-7 2013 Finally, fibrin(ogen)-Sepharose chromatography indicated that A1AT purified from plasma contained a small fraction of fibrin(ogen)-binding A1AT. Sepharose 22-31 serpin family A member 1 Homo sapiens 62-66 23800469-2 2013 We found that the Rap guanine nucleotide exchange factor Rapgef2 was enriched from primary bovine neuroendocrine cells by cAMP-agarose affinity chromatography and that it was specifically eluted by cAMP. Sepharose 127-134 Rap guanine nucleotide exchange factor 2 Bos taurus 57-64 23575353-6 2013 We further observed that decreasing binding effects between the risk alleles A of IL12B and CCAAT/enhancer binding protein alpha (C/EBPalpha) through A allele sequence mediated streptavidin-conjugated agarose pulldown and biotin-labelled A allele mediated electrophoretic mobility shift assay. Sepharose 201-208 interleukin 12B Homo sapiens 82-87 23575353-6 2013 We further observed that decreasing binding effects between the risk alleles A of IL12B and CCAAT/enhancer binding protein alpha (C/EBPalpha) through A allele sequence mediated streptavidin-conjugated agarose pulldown and biotin-labelled A allele mediated electrophoretic mobility shift assay. Sepharose 201-208 CCAAT enhancer binding protein alpha Homo sapiens 92-128 23575353-6 2013 We further observed that decreasing binding effects between the risk alleles A of IL12B and CCAAT/enhancer binding protein alpha (C/EBPalpha) through A allele sequence mediated streptavidin-conjugated agarose pulldown and biotin-labelled A allele mediated electrophoretic mobility shift assay. Sepharose 201-208 CCAAT enhancer binding protein alpha Homo sapiens 130-140 23371442-1 2013 Diamine oxidase (DAO) was purified to homogeneity from human seminal plasma by consecutive chromatographic fractionation on heparin-sepharose, phenyl-sepharose, CIM-QA, and Superdex 200. Sepharose 132-141 amine oxidase copper containing 1 Homo sapiens 0-15 23371442-1 2013 Diamine oxidase (DAO) was purified to homogeneity from human seminal plasma by consecutive chromatographic fractionation on heparin-sepharose, phenyl-sepharose, CIM-QA, and Superdex 200. Sepharose 132-141 amine oxidase copper containing 1 Homo sapiens 17-20 23709562-6 2013 Incubation of FAMP5 with human HDL or whole plasma generated small HDL particles, and charged apoA-I-rich particles migrated as pre-beta HDL on agarose gel electrophoresis. Sepharose 144-151 apolipoprotein A1 Homo sapiens 94-100 23390139-6 2013 SP-D lectin activity was also tested towards maltose-agarose and mannan for selected BALF samples. Sepharose 53-60 surfactant protein D Homo sapiens 0-4 23500722-5 2013 After purification with Ni-NTA agarose column, recombinant cecropinXJ was noted to exert strong antimicrobial activities against a broad-spectrum of microorganisms, including Gram-negative and Gram-positive bacteria. Sepharose 31-38 cecropin-B Bombyx mori 59-69 23828379-6 2013 RESULTS: Rec-hBD-4 was expressed in bacterial cells as GST-hBD-4 fusion protein, and purified by routine 3-step procedure (affine chromatography on glutathione-agarose, cleavage of fusion protein by thrombin, and reverse phase chromatography). Sepharose 160-167 defensin beta 104B Homo sapiens 13-18 22233542-1 2013 Serum paraoxonase 1 (PON1) was purified from bovine serum using hydrophobic interaction chromotography on Sepharose 4B-coupled l-tyrosine 1-naphthylamine gel, and monitored by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Sepharose 106-118 paraoxonase 1 Homo sapiens 21-25 23624571-6 2013 SenX3 expression from the resulting pET-28b-mSenX3 plasmid was induced with isopropyl beta-D-thiogalactoside and the protein was purified using Ni-NTA agarose affinity chromatography. Sepharose 151-158 two component sensor histidine kinase SenX3 Mycobacterium tuberculosis H37Rv 0-5 23523510-8 2013 Using a pull down assay in rat brain tissue homogenate with gelatine-agarose beads, we showed increased activities for both the pro and mature forms of MMP-2 and MMP-9. Sepharose 69-76 matrix metallopeptidase 2 Rattus norvegicus 152-157 23523510-8 2013 Using a pull down assay in rat brain tissue homogenate with gelatine-agarose beads, we showed increased activities for both the pro and mature forms of MMP-2 and MMP-9. Sepharose 69-76 matrix metallopeptidase 9 Rattus norvegicus 162-167 23704877-5 2013 We used semi-denaturing detergent-agarose gel electrophoresis to isolate high molecular weight aggregates containing TDP-43 that are present in the cerebral cortex of individuals with frontotemporal lobar degeneration but not that of controls. Sepharose 34-41 TAR DNA binding protein Homo sapiens 117-123 22915152-2 2013 An extracellular chitinase, produced by antagonistic S. violaceusniger MTCC 3959, was purified as follows: ammonium sulfate precipitation, chitin affinity and chromatographic separation of Q Sepharose. Sepharose 191-200 PI-PLC domain-containing protein Streptomyces albidoflavus 17-26 23385154-4 2013 A single injection of SPC-54 (10mg/kg) neutralized circulating PC in mice for at least 7days, and immunoblotting and immuno-precipitation with protein G-agarose confirmed that SPC-54 in vivo was bound to PC in plasma. Sepharose 153-160 sparse coat Mus musculus 176-179 23340442-5 2013 VWF:CB was analyzed with type III collagen and multimer distribution by agarose gel electrophoresis. Sepharose 72-79 von Willebrand factor Homo sapiens 0-6 23454515-3 2013 In order to investigate the scope of potential targets of Leucettine L41, a representative member of the chemical class, we designed an affinity chromatography strategy based on agarose-immobilized leucettines. Sepharose 178-185 skull morphology 5 Mus musculus 69-72 23454515-6 2013 L41 was then covalently bound to agarose beads through the primary amine located at the end of the linker. Sepharose 33-40 skull morphology 5 Mus musculus 0-3 23485472-4 2013 SSL8-conjugated Sepharose specifically recovered tenascin C (TNC), a multimodular and multifunctional extracellular matrix protein. Sepharose 16-25 tenascin C Homo sapiens 49-59 23485472-4 2013 SSL8-conjugated Sepharose specifically recovered tenascin C (TNC), a multimodular and multifunctional extracellular matrix protein. Sepharose 16-25 tenascin C Homo sapiens 61-64 23485472-5 2013 Pull down analysis using SSL8-conjugated Sepharose and recombinant truncated fragments of TNC revealed that SSL8 interacts with fibronectin (FN) type III repeats 1-5 of TNC. Sepharose 41-50 fibronectin 1 Homo sapiens 128-139 23496967-3 2013 Lp(a) phenotypes were classified into 7 subtypes according to sodium dodecyl sulfate-agarose gel electrophoresis by Western blotting. Sepharose 85-92 lipoprotein(a) Homo sapiens 0-5 23374512-11 2013 The isolate CP2 revealed four bands: one of large molecular size ~56.4 kb and three of small size ~6.5 kb, ~6.1 kb and ~1.5 kb by agarose gel electrophoresis indicating the presence of 3 plasmids. Sepharose 130-137 ceruloplasmin Homo sapiens 12-15 23090015-6 2013 We further demonstrated that HSC70 is a MTX-binding protein using a binding assay with MTX-agarose beads followed by Western blotting. Sepharose 91-98 heat shock protein 8 Mus musculus 29-34 23053742-6 2013 The conditioned medium of monocytes also potentiated MKN1 cell invasion; however, the potentiation was lowered by the depletion of tumor necrosis factor (TNF)-alpha from the conditioned medium with an antibody-protein G-Sepharose conjugate. Sepharose 220-229 tumor necrosis factor Homo sapiens 131-164 23554536-0 2013 Efficient Genetic Analysis of Microdissected Samples by Agarose-Bead Method: Alterations of beta-Catenin Gene in Fundic Gland Polyp and Heterotopic Gastric Mucosa of Duodenum. Sepharose 56-63 catenin beta 1 Homo sapiens 92-104 23554536-3 2013 In the present study, to elucidate the contribution of beta-catenin gene mutation to the fundic gland polyp and the heterotopic gastric mucosa in the duodenum, we successfully introduced an agarose-bead mediated technique as an effectual tool for retrospective morphology-oriented genetic analyses. Sepharose 190-197 catenin beta 1 Homo sapiens 55-67 23856142-3 2013 The transcription of Bcl-2 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) by agarose gel electrophoresis, real-time PCR, and the protein level of Bcl-2 was measured by Western blot to confirm the function of miRNA plasmid. Sepharose 108-115 BCL2 apoptosis regulator Homo sapiens 21-26 23358444-7 2013 Specific interactions of MTX-conjugated QDs with DHFR were identified using agarose gel electrophoresis and fluorescence microscopy. Sepharose 76-83 dihydrofolate reductase Cricetulus griseus 49-53 23457905-5 2013 Agarose gel electrophoresis was used to identify eNOS gene polymorphisms. Sepharose 0-7 nitric oxide synthase 3 Homo sapiens 49-53 23182350-4 2013 Accordingly, in the present study we examined whether template agarose scaffolds seeded with bone marrow stromal cells secreting Brain-Derived Neurotrophic Factor (BDNF) would support regeneration into severe, complete spinal cord transection sites. Sepharose 63-70 brain derived neurotrophic factor Homo sapiens 164-168 23106544-4 2013 SNPs at the promoter regions -376G/A, -238G/A and -308G/A of the TNF gene and the Asp299Gly and Thr399Ile SNPs of the TLR4 gene were determined by polymerase chain reaction (PCR) and digestion of the PCR product by restriction enzymes; after electrophoresis on 2 0% agarose gel, products were visualized on under ultraviolet radiation. Sepharose 266-273 toll like receptor 4 Homo sapiens 118-122 23258531-4 2013 Here, we show that miR-31 is upregulated in human colon cancer tissues and cell lines, and that repression of miR-31 inhibited colon cancer cell proliferation and colony formation in soft agarose. Sepharose 212-219 microRNA 31 Homo sapiens 122-128 23258531-9 2013 The results of MTT and soft agarose colony-formation assays showed that knockdown of RhoBTB1 by RNAi induced cell proliferation, and colony formation in soft agarose, which mimicked the function of miR-31. Sepharose 40-47 Rho related BTB domain containing 1 Homo sapiens 109-116 23258531-9 2013 The results of MTT and soft agarose colony-formation assays showed that knockdown of RhoBTB1 by RNAi induced cell proliferation, and colony formation in soft agarose, which mimicked the function of miR-31. Sepharose 182-189 Rho related BTB domain containing 1 Homo sapiens 109-116 23552777-4 2013 Finally, the recombinant vector pLentiTrident1-hBMP2-Neo-hNGF was confirmed by agarose gel electrophoresis and DNA sequence analysis. Sepharose 79-86 bone morphogenetic protein 2 Homo sapiens 47-52 23552777-4 2013 Finally, the recombinant vector pLentiTrident1-hBMP2-Neo-hNGF was confirmed by agarose gel electrophoresis and DNA sequence analysis. Sepharose 79-86 nerve growth factor Homo sapiens 57-61 22677456-5 2013 In this study, we established an Escherichia coli expression system, which produced a large amount of tagged human liver prolidase that was purified to over 95% purity from the soluble fraction of cell lysate by affinity chromatography on Streptavidin-agarose resin. Sepharose 252-259 peptidase D Homo sapiens 121-130 22289623-4 2013 Agarose gel retardation assays demonstrated that PPD could encapsulate plasmid DNA completely when the N/P ratio is equal to or above 3. Sepharose 0-7 cellular communication network factor 6 Homo sapiens 49-52 24312807-0 2013 Affinity Purification of Tumor Necrosis Factor-alpha Expressed in Raji Cells by Produced scFv Antibody Coupled CNBr-Activated Sepharose. Sepharose 126-135 tumor necrosis factor Homo sapiens 25-52 24312807-0 2013 Affinity Purification of Tumor Necrosis Factor-alpha Expressed in Raji Cells by Produced scFv Antibody Coupled CNBr-Activated Sepharose. Sepharose 126-135 immunglobulin heavy chain variable region Homo sapiens 89-93 24312807-7 2013 The anti-TNF-alpha scFv selected from antibody phage display library was coupled to CNBr-activated sepharose 4B beads used for affinity purification of expressed TNF-alpha and the purity of the protein was assessed by SDS-PAGE. Sepharose 99-111 tumor necrosis factor Homo sapiens 9-18 24312807-7 2013 The anti-TNF-alpha scFv selected from antibody phage display library was coupled to CNBr-activated sepharose 4B beads used for affinity purification of expressed TNF-alpha and the purity of the protein was assessed by SDS-PAGE. Sepharose 99-111 immunglobulin heavy chain variable region Homo sapiens 19-23 24312807-7 2013 The anti-TNF-alpha scFv selected from antibody phage display library was coupled to CNBr-activated sepharose 4B beads used for affinity purification of expressed TNF-alpha and the purity of the protein was assessed by SDS-PAGE. Sepharose 99-111 tumor necrosis factor Homo sapiens 162-171 23139417-5 2012 When compared with wild-type CRP, mutant CRP bound more avidly to phosphoethanolamine and could be purified by affinity chromatography using phosphoethanolamine-conjugated Sepharose. Sepharose 172-181 C-reactive protein, pentraxin-related Mus musculus 41-44 23520443-1 2013 IgGs from patients with multiple sclerosis and systemic lupus erythematosus (SLE) purified on MBP-Sepharose in contrast to canonical proteases hydrolyze effectively only myelin basic protein (MBP), but not many other tested proteins. Sepharose 98-107 myelin basic protein Homo sapiens 94-97 23520443-1 2013 IgGs from patients with multiple sclerosis and systemic lupus erythematosus (SLE) purified on MBP-Sepharose in contrast to canonical proteases hydrolyze effectively only myelin basic protein (MBP), but not many other tested proteins. Sepharose 98-107 myelin basic protein Homo sapiens 170-190 23520443-1 2013 IgGs from patients with multiple sclerosis and systemic lupus erythematosus (SLE) purified on MBP-Sepharose in contrast to canonical proteases hydrolyze effectively only myelin basic protein (MBP), but not many other tested proteins. Sepharose 98-107 myelin basic protein Homo sapiens 192-195 23601467-5 2013 PCR and agarose gel electrophoresis methods were adopted to determine the rs1799752 I/D polymorphism genotypes of the ACE gene. Sepharose 8-15 angiotensin I converting enzyme Homo sapiens 118-121 23319971-10 2012 The band of TSLC1 mRNA of TTG was located at about 1400 bp by RT-PCR and agarose gel electrophoresis assay. Sepharose 73-80 cell adhesion molecule 1 Homo sapiens 12-17 23181906-5 2012 Furthermore, they were covalently linked to Sepharose and used as an affinity matrix for ovalbumin depletion. Sepharose 44-53 ovalbumin (SERPINB14) Gallus gallus 89-98 23169343-5 2012 The recombinant cocoonase was purified to homogeneity by 80% ammonium-sulfate fractionation and CM-Sepharose chromatography, and its internal peptide sequences were analyzed by nano liquid chromatography-mass spectrometry/mass spectrometry. Sepharose 99-108 cocoonase Bombyx mori 16-25 22831549-5 2012 The mechanisms for H(2) S-regulated HIF-1alpha protein levels were determined using short interfering RNA transfection, co-immunoprecipitation and 7-methyl-GTP sepharose 4B pull-down assay. Sepharose 160-169 hypoxia inducible factor 1 subunit alpha Homo sapiens 36-46 22993214-2 2012 The affinity matrix comprises a bacterially expressed, recombinant protein, the TOG1/2 domains from Saccharomyces cerevisiae Stu2, covalently coupled to a Sepharose support. Sepharose 155-164 Stu2p Saccharomyces cerevisiae S288C 125-129 22802125-8 2012 Following up on this finding, we show that the alpha1-alpha3-Fc structure coated on agarose beads is tolerogenic and capable of prolonging the survival of skin allografts in B6-mice and in a LILRB2-transgenic mouse model. Sepharose 84-91 cholinergic receptor, nicotinic, alpha polypeptide 3 Mus musculus 47-60 23279476-0 2012 Cat and dog primordial follicles enclosed in ovarian cortex sustain viability after in vitro culture on agarose gel in a protein-free medium. Sepharose 104-111 catalase Canis lupus familiaris 0-3 23279476-10 2012 For the cat, the agarose gel better (p < 0.05) supported follicle viability compared to the control, but was equivalent to the cell culture insert. Sepharose 17-24 catalase Canis lupus familiaris 8-11 23232521-3 2012 Two recombinant prokaryotic proteins of VSTM1-v2, Trx-His-S-VSTM1-v2 and GST-VSTM1-v2, were constructed, expressed, purified, and then used for immunization of New Zealand rabbits to prepare anti-VSTM1 antibody and coupling with CNBr-activated Sepharose 4B to purify the antibody by immunoaffinity chromatography, respectively. Sepharose 249-258 V-set and transmembrane domain-containing protein 1 Oryctolagus cuniculus 40-48 23232521-3 2012 Two recombinant prokaryotic proteins of VSTM1-v2, Trx-His-S-VSTM1-v2 and GST-VSTM1-v2, were constructed, expressed, purified, and then used for immunization of New Zealand rabbits to prepare anti-VSTM1 antibody and coupling with CNBr-activated Sepharose 4B to purify the antibody by immunoaffinity chromatography, respectively. Sepharose 249-258 V-set and transmembrane domain-containing protein 1 Oryctolagus cuniculus 40-45 23627031-3 2012 METHODS: We express kod-ssb with the Transrtta (DE3), and kod-ssb was purified by affinity chromatography on a Ni2+ Sepharose column, detected by SDS-PAGE. Sepharose 116-125 small RNA binding exonuclease protection factor La Homo sapiens 62-65 22759790-8 2012 Evidence for the presence of a signaling complex in vivo was obtained by purifying betaAR from detergent extracts of mouse brain with alprenolol-Sepharose and showing that the precipitate also contained both D(2)-like dopamine receptors and AC. Sepharose 145-154 adrenergic receptor, beta 1 Mus musculus 83-89 30727467-8 2012 PstI yielded a fragment of ~1.8 kb when the digested product was analyzed by electrophoresis on a 1% agarose gel. Sepharose 101-108 serine peptidase inhibitor Kazal type 1 Homo sapiens 0-4 22587667-0 2012 Adsorption of chain type-specific ABO antibodies on Sepharose-linked A and B tetrasaccharides. Sepharose 52-61 ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Homo sapiens 34-37 22705044-8 2012 Interestingly, treatment of DCs with agarose film maintained the DC functional phenotype at levels similar to iDCs except for CD44 expression, which was lower than that of iDCs. Sepharose 37-44 CD44 molecule (Indian blood group) Homo sapiens 126-130 22714809-4 2012 The spent medium of pcDNA3.0-TAT-CT3-cMyc-transfected CHO cells could be purified using a cMyc-epitope-tag agarose affinity chromatography column and could be detected via SDS-PAGE, with antibodies against cMyc-tag. Sepharose 107-114 cancer antigen 1 Homo sapiens 33-36 22714809-4 2012 The spent medium of pcDNA3.0-TAT-CT3-cMyc-transfected CHO cells could be purified using a cMyc-epitope-tag agarose affinity chromatography column and could be detected via SDS-PAGE, with antibodies against cMyc-tag. Sepharose 107-114 MYC proto-oncogene, bHLH transcription factor Homo sapiens 37-41 22714809-4 2012 The spent medium of pcDNA3.0-TAT-CT3-cMyc-transfected CHO cells could be purified using a cMyc-epitope-tag agarose affinity chromatography column and could be detected via SDS-PAGE, with antibodies against cMyc-tag. Sepharose 107-114 MYC proto-oncogene, bHLH transcription factor Homo sapiens 90-94 22714809-4 2012 The spent medium of pcDNA3.0-TAT-CT3-cMyc-transfected CHO cells could be purified using a cMyc-epitope-tag agarose affinity chromatography column and could be detected via SDS-PAGE, with antibodies against cMyc-tag. Sepharose 107-114 MYC proto-oncogene, bHLH transcription factor Homo sapiens 90-94 25005977-2 2012 Three-step purification - by ammonium sulphate precipitation, Sephadex G-100, and Q Sepharose - was applied to isolate trypsin, and resulted in 3.77% recovery with a 5.34-fold increase in specific activity. Sepharose 84-93 serine protease 1 Oreochromis niloticus 119-126 22623052-3 2012 PV was immobilized on agarose beads with divinyl sulfone. Sepharose 22-29 casein kinase 2 beta Homo sapiens 0-2 22623052-4 2012 Five aliquots of agarose-immobilized PV, acidic monomers, and their calcium salts were incubated in mineralizing solution at various concentrations. Sepharose 17-24 casein kinase 2 beta Homo sapiens 37-39 23107735-9 2012 beta-Galactosidase also exhibited glycoproteineous properties when applied on Con-A Sepharose column. Sepharose 84-93 galactosidase beta 1 Gallus gallus 0-18 22759380-6 2012 Exogenous growth differentiation factor (GDF)-9 reversed these heparin effects; furthermore, GDF9 strongly bound to heparin sepharose. Sepharose 124-133 growth differentiation factor 9 Homo sapiens 10-47 22759380-6 2012 Exogenous growth differentiation factor (GDF)-9 reversed these heparin effects; furthermore, GDF9 strongly bound to heparin sepharose. Sepharose 124-133 growth differentiation factor 9 Homo sapiens 93-97 22852885-2 2012 METHODS: The VDR gene polymorphisms BsmI and TaqI were detected by polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP)-genotyping assays by using endonucleases BsmI and TaqI, and an agarose gel electrophoresis technique in a series of 43 colon cancer patients and 42 healthy controls. Sepharose 211-218 vitamin D receptor Homo sapiens 13-16 22652662-5 2012 The recombinant GST-FMRP was purified on a glutathione sepharose 4B affinity column and detected using SDS-PAGE followed by western blotting with anti-FMRP antibody. Sepharose 55-64 fragile X messenger ribonucleoprotein 1 Homo sapiens 20-24 22826003-4 2012 Agarose gel electrophoresis was used to asses DNA binding and perform a DNase I protection assay. Sepharose 0-7 deoxyribonuclease I Mus musculus 72-79 22781164-1 2012 In contrast to canonical proteases, myelin basic protein (MBP)-Sepharose-purified IgG from multiple sclerosis (MS) and systemic lupus erythematosus (SLE) patients efficiently hydrolyze only MBP, but not many other tested proteins. Sepharose 63-72 myelin basic protein Homo sapiens 36-56 22781164-1 2012 In contrast to canonical proteases, myelin basic protein (MBP)-Sepharose-purified IgG from multiple sclerosis (MS) and systemic lupus erythematosus (SLE) patients efficiently hydrolyze only MBP, but not many other tested proteins. Sepharose 63-72 myelin basic protein Homo sapiens 58-61 22781164-1 2012 In contrast to canonical proteases, myelin basic protein (MBP)-Sepharose-purified IgG from multiple sclerosis (MS) and systemic lupus erythematosus (SLE) patients efficiently hydrolyze only MBP, but not many other tested proteins. Sepharose 63-72 myelin basic protein Homo sapiens 190-193 22818772-2 2012 We have herein observed that positively charged proteins (lysozyme and cytochrome c) are adsorbed on the agarose-based anion-exchangers (Q and DEAE Sepharose FF gels) in a capacity of 10-40 mug/mL. Sepharose 105-112 cytochrome c, somatic Homo sapiens 71-83 22818772-2 2012 We have herein observed that positively charged proteins (lysozyme and cytochrome c) are adsorbed on the agarose-based anion-exchangers (Q and DEAE Sepharose FF gels) in a capacity of 10-40 mug/mL. Sepharose 148-157 cytochrome c, somatic Homo sapiens 71-83 22665377-4 2012 An approximately 100-kDa protein was recovered by using recombinant His-tagged SSL3-conjugated Sepharose from the lysate of porcine spleen, and the protein was identified as porcine TLR2 by peptide mass fingerprinting analysis. Sepharose 95-104 toll-like receptor 2 Mus musculus 182-186 22665377-5 2012 The SSL3-conjugated Sepharose recovered human and mouse TLR2 but not TLR4 from human neutrophils and mouse macrophage RAW 264.7 cells, as well as a recombinant TLR2 extracellular domain chimera protein. Sepharose 20-29 toll-like receptor 2 Mus musculus 56-60 22665377-5 2012 The SSL3-conjugated Sepharose recovered human and mouse TLR2 but not TLR4 from human neutrophils and mouse macrophage RAW 264.7 cells, as well as a recombinant TLR2 extracellular domain chimera protein. Sepharose 20-29 toll-like receptor 2 Mus musculus 160-164 22875513-4 2012 The coding regions of CCR5 gene were amplified with PCR followed by agarose gel electrophoresis. Sepharose 68-75 C-C motif chemokine receptor 5 Homo sapiens 22-26 22750863-2 2012 The extracellular region of mouse Enpp1 was expressed in HEK293S GnT1(-) cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. Sepharose 116-125 ectonucleotide pyrophosphatase/phosphodiesterase 1 Mus musculus 34-39 22613789-6 2012 The scFv was purified by immobilized metal affinity chromatography (IMAC) on a nickel-nitrilotriacetic acid (NTA) agarose column and characterized. Sepharose 114-121 immunglobulin heavy chain variable region Homo sapiens 4-8 22658159-13 2012 In conclusion, expanding MSCs in the presence of FGF-2 rapidly accelerates chondrogenesis in 3D agarose cultures resulting in superior mechanical functionality. Sepharose 96-103 fibroblast growth factor 2 Homo sapiens 49-54 22634004-8 2012 Photocrosslinking and neutravidin-agarose capture experiments with these analogs revealed that both hIcmt and Ste14p were specifically photolabeled to varying degrees with all of the compounds tested. Sepharose 34-41 isoprenylcysteine carboxyl methyltransferase Homo sapiens 100-105 22618232-6 2012 Here, we demonstrate that IFI16 protein binds strongly to negatively superhelical plasmid DNA at a native superhelix density, as evidenced by electrophoretic retardation of supercoiled (sc) DNA in agarose gels. Sepharose 197-204 interferon gamma inducible protein 16 Homo sapiens 26-31 22703118-10 2012 A pull-down assay with glutathione S-transferase (GST)-agarose beads followed by Western blot analysis was employed to confirm microtubule S-glutathionylation. Sepharose 55-62 glutathione S-transferase kappa 1 Homo sapiens 23-48 22703118-10 2012 A pull-down assay with glutathione S-transferase (GST)-agarose beads followed by Western blot analysis was employed to confirm microtubule S-glutathionylation. Sepharose 55-62 glutathione S-transferase kappa 1 Homo sapiens 50-53 22528491-10 2012 In addition, native mGluR4 was retained on a Sepharose column covalently grafted with recombinant Munc18-1, and immunohistochemistry experiments showed that Munc18-1 and mGluR4 colocalized at plasma membrane in HEK293 cells, observations in favor of an interaction between the two proteins. Sepharose 45-54 glutamate receptor, ionotropic, AMPA4 (alpha 4) Mus musculus 20-26 22528491-10 2012 In addition, native mGluR4 was retained on a Sepharose column covalently grafted with recombinant Munc18-1, and immunohistochemistry experiments showed that Munc18-1 and mGluR4 colocalized at plasma membrane in HEK293 cells, observations in favor of an interaction between the two proteins. Sepharose 45-54 syntaxin binding protein 1 Homo sapiens 98-106 22528491-10 2012 In addition, native mGluR4 was retained on a Sepharose column covalently grafted with recombinant Munc18-1, and immunohistochemistry experiments showed that Munc18-1 and mGluR4 colocalized at plasma membrane in HEK293 cells, observations in favor of an interaction between the two proteins. Sepharose 45-54 syntaxin binding protein 1 Homo sapiens 157-165 22304787-2 2012 In this study, we show that prohibitin is up-regulated in lung squamous cell carcinoma tissues compared with adjacent normal tissues using agarose 2-dimensional differential gel electrophoresis and immunoblotting. Sepharose 139-146 prohibitin 1 Homo sapiens 28-38 22791371-5 2012 Short-term sliding contact of mesenchymal stem cell (MSC)-seeded agarose improved chondrogenic gene expression in a manner dependent on both the axial strain applied and transforming growth factor-beta supplementation. Sepharose 65-72 musculin Homo sapiens 53-56 22791371-6 2012 Using the optimized loading parameters derived from these short-term studies, long-term sliding contact was applied to MSC-seeded agarose constructs for 21 d. After 21 d, sliding contact significantly improved the tensile properties of MSC-seeded constructs and elicited alterations in type II collagen and proteoglycan accumulation as a function of depth; staining for these matrix molecules showed intense localization in the surface regions. Sepharose 130-137 musculin Homo sapiens 119-122 22791371-6 2012 Using the optimized loading parameters derived from these short-term studies, long-term sliding contact was applied to MSC-seeded agarose constructs for 21 d. After 21 d, sliding contact significantly improved the tensile properties of MSC-seeded constructs and elicited alterations in type II collagen and proteoglycan accumulation as a function of depth; staining for these matrix molecules showed intense localization in the surface regions. Sepharose 130-137 musculin Homo sapiens 236-239 22630171-8 2012 Compared to the conventional agarose gel-chromatography, the AuNP-based probe exhibited less nonspecific interference and higher recovery yield for ERalpha. Sepharose 29-36 estrogen receptor 1 Homo sapiens 148-155 22378108-8 2012 RESULTS: Mucin (MUC)5AC polymers exceeding 260 kDa were observed in agarose gels; NuPAGE resolved polymers from 260 to 3.5 kDa: when large mucins were detected, the smallest fragments were missing. Sepharose 68-75 LOC100508689 Homo sapiens 9-14 22378108-8 2012 RESULTS: Mucin (MUC)5AC polymers exceeding 260 kDa were observed in agarose gels; NuPAGE resolved polymers from 260 to 3.5 kDa: when large mucins were detected, the smallest fragments were missing. Sepharose 68-75 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 16-23 22404109-5 2012 Like HDA14, the putative histone acetyltransferase ELP3 was purified on microcystin-Sepharose and is also enriched at microtubules, potentially functioning in opposition to HDA14 as the alpha-tubulin acetylating enzyme. Sepharose 84-93 radical SAM domain-containing protein / GCN5-related N-acetyltransferase (GNAT) family protein Arabidopsis thaliana 51-55 22589334-1 2012 Cap-binding proteins have been routinely isolated using m7GTP-Sepharose; however, this resin is inefficient for proteins such as DcpS (scavenger decapping enzyme), which interacts not only with the 7-methylguanosine, but also with the second cap base. Sepharose 62-71 decapping enzyme, scavenger Homo sapiens 129-133 22589334-3 2012 Here, we report the synthesis of new affinity resins, m7GpCH2pp- and m7GpCH2ppA-Sepharoses, with attached cap analogs resistant to hydrolysis by DcpS. Sepharose 80-90 decapping enzyme, scavenger Homo sapiens 145-149 22589334-5 2012 In addition, purification of cap-binding proteins from yeast extracts confirmed the presence of all expected cap-binding proteins, including DcpS in the case of m7GpCH2pp- and m7GpCH2ppA-Sepharoses. Sepharose 187-197 decapping enzyme, scavenger Homo sapiens 141-145 22589334-6 2012 In contrast, binding studies in vitro demonstrated that recombinant human DcpS efficiently bound only m7GpCH2ppA-Sepharose. Sepharose 113-122 decapping enzyme, scavenger Homo sapiens 74-78 22807932-4 2012 MnSOD enzyme activity was determined using an indirect competitive inhibition assay and MnSOD gene polymorphism using poly merase chain reaction and agarose gel electrophoresis. Sepharose 149-156 superoxide dismutase 2 Homo sapiens 0-5 22740250-0 2012 Tris(hydroxymethyl)aminomethane-functionalized agarose particles: parameters affecting the binding of bovine serum albumin. Sepharose 47-54 albumin Homo sapiens 109-122 22542587-8 2012 By contrast, urea-denaturated ATAD3A-Myc-HIS bound to agarose-nickel beads and could be renatured and eluted to obtain highly pure ATAD3A-Myc-HIS. Sepharose 54-61 ATPase family AAA domain containing 3A Homo sapiens 30-36 22542587-8 2012 By contrast, urea-denaturated ATAD3A-Myc-HIS bound to agarose-nickel beads and could be renatured and eluted to obtain highly pure ATAD3A-Myc-HIS. Sepharose 54-61 MYC proto-oncogene, bHLH transcription factor Homo sapiens 37-40 23016297-6 2012 The yield of F VIII in superporous chromatography was about five times of commercially agarose chromatography and the specific activity was up to 154 IU/mg protein. Sepharose 87-94 coagulation factor VIII Homo sapiens 13-19 22803352-5 2012 The expressed recombinant SP-A was identified by Western blot and purified from culture medium by Ni-NTA-Agarose beads. Sepharose 105-112 surfactant protein A1 Homo sapiens 26-30 22336033-7 2012 In addition, after nucleic acid analysis, fertile blastodiscs yielded much stronger chromosomal DNA and CHD1-targeted PCR bands on agarose gels compared with infertile blastodiscs. Sepharose 131-138 chromodomain helicase DNA binding protein 1 Homo sapiens 104-108 22239246-3 2012 The VWF multimeric pattern was studied by agarose gel electrophoresis. Sepharose 42-49 von Willebrand factor Homo sapiens 4-7 22490510-5 2012 In the p-BNCs, CA125 from diseased sera (Bio) is sequestered and assessed with a fluorescence-based sandwich immunoassay, completed in the nano-nets (Nano) of sensitized agarose microbeads localized in individually addressable wells (Chip), housed in a microfluidic module, capable of integrating multiple sample, reagent and biowaste processing, and handling steps. Sepharose 170-177 mucin 16, cell surface associated Homo sapiens 15-20 22025420-4 2012 Fibrin was formed by the addition of thrombin to fibrinogen in 0.2% agarose gel. Sepharose 68-75 coagulation factor II, thrombin Homo sapiens 37-45 22297031-6 2012 MAIN OUTCOME MEASURES: Goblet cells in the corneal epithelium were detected by light microscopy, and the MUC5AC transcript was detected as the corresponding PCR amplicon in agarose gels. Sepharose 173-180 mucin 5AC, oligomeric mucus/gel-forming Homo sapiens 105-111 22888537-3 2012 The optimum reaction parameters were determined as pH 5.0, a molar ratio of mPEG-ALD2000 to L-asparaginase of 10:1, a reaction time of 16 h and temperature of 25 degrees C. PEG-L-asparaginase (PEG-L-ASNase) was isolated and purified with consecutive anion-exchange (XK, 16 x 20 cm, Q Sepharose FF) and gel-filtration (Tricorn, 10 x 600 cm, Sephacryl S-300 HR) chromatography, respectively. Sepharose 284-293 asparaginase and isoaspartyl peptidase 1 Homo sapiens 177-191 22238349-10 2012 Based on our findings, we developed a procedure for the purification of H-ficolin from serum, involving PEG precipitation, affinity chromatography on Sepharose derivatized with acetylated serum albumin, ion exchange chromatography, and gel permeation chromatography. Sepharose 150-159 ficolin 3 Homo sapiens 72-81 22558991-2 2012 METHODS: The plasmid of pET32a(+)LC3 was successfully constructed, human LC3 protein was purified from E.coli through one-step purification with SP-Sepharose XL. Sepharose 149-158 microtubule associated protein 1 light chain 3 alpha Homo sapiens 74-77 22433083-0 2012 Solid-phase N-terminus PEGylation of recombinant human fibroblast growth factor 2 on heparin-sepharose column. Sepharose 93-102 fibroblast growth factor 2 Homo sapiens 55-81 22434709-4 2012 A similar situation was observed for IgG from patients with multiple sclerosis and HIV-infected patients, which after purification on myelin basic protein (MBP)-Sepharose and RT-Sepharose specifically hydrolyze only MBP and RT, respectively. Sepharose 161-170 myelin basic protein Homo sapiens 134-154 22434709-4 2012 A similar situation was observed for IgG from patients with multiple sclerosis and HIV-infected patients, which after purification on myelin basic protein (MBP)-Sepharose and RT-Sepharose specifically hydrolyze only MBP and RT, respectively. Sepharose 161-170 myelin basic protein Homo sapiens 156-159 22434709-4 2012 A similar situation was observed for IgG from patients with multiple sclerosis and HIV-infected patients, which after purification on myelin basic protein (MBP)-Sepharose and RT-Sepharose specifically hydrolyze only MBP and RT, respectively. Sepharose 178-187 myelin basic protein Homo sapiens 216-219 22448747-6 2012 However, digestion of HSP70 with Proteinase K-agarose beads also dramatically reduced the TNF-alpha response of macrophages to HSP70, while leaving levels of contaminating endotoxin largely unchanged relative to controls. Sepharose 46-53 heat shock protein family A (Hsp70) member 4 Homo sapiens 22-27 22448747-6 2012 However, digestion of HSP70 with Proteinase K-agarose beads also dramatically reduced the TNF-alpha response of macrophages to HSP70, while leaving levels of contaminating endotoxin largely unchanged relative to controls. Sepharose 46-53 endogenous retrovirus group K member 25 Homo sapiens 33-43 22448747-6 2012 However, digestion of HSP70 with Proteinase K-agarose beads also dramatically reduced the TNF-alpha response of macrophages to HSP70, while leaving levels of contaminating endotoxin largely unchanged relative to controls. Sepharose 46-53 tumor necrosis factor Homo sapiens 90-99 22448747-6 2012 However, digestion of HSP70 with Proteinase K-agarose beads also dramatically reduced the TNF-alpha response of macrophages to HSP70, while leaving levels of contaminating endotoxin largely unchanged relative to controls. Sepharose 46-53 heat shock protein family A (Hsp70) member 4 Homo sapiens 127-132 24956524-4 2012 An agarose-carbomer (AC1) hydrogel, already used in SCI repair strategies, was here investigated as a delivery system capable of an effective chABC administration: the material ability to include chABC within its pores and the possibility to be injected into the target tissue were firstly proved. Sepharose 3-10 long intergenic non-protein coding RNA 1587 Homo sapiens 21-24 22357208-8 2012 A semi-quantitative agarose-gel-based ribonuclease assay was developed for measuring its 2-5A-dependent RNase L activity against cellular large rRNAs as substrates. Sepharose 20-27 ribonuclease L Homo sapiens 104-111 22699735-0 2012 The ferric aerobactin receptor IutA, a protein isolated on agarose column, is not essential for uropathogenic Escherichia coli infection. Sepharose 59-66 Aerobactin siderophore ferric receptor protein IutA Escherichia coli 31-35 22394631-3 2012 The fusion protein GST-Cdc25C was expressed in E.coli Rossate and purified by GST-Sepharose 4B beads. Sepharose 82-94 cell division cycle 25C Homo sapiens 23-29 22781599-4 2012 Compared with WT-VWF, the susceptibility of A1500E mutant VWF to proteolysis by ADAMTS13 was analyzed using SDS-agarose gel VWF multimers analysis. Sepharose 112-119 ADAM metallopeptidase with thrombospondin type 1 motif 13 Homo sapiens 80-88 22781599-4 2012 Compared with WT-VWF, the susceptibility of A1500E mutant VWF to proteolysis by ADAMTS13 was analyzed using SDS-agarose gel VWF multimers analysis. Sepharose 112-119 von Willebrand factor Homo sapiens 58-61 22104730-6 2012 The MMP-9 production from monocytes was diminished by the depletion of fibronectin from the conditioned media with gelatin-Sepharose, and potentiated by culturing them in fibronectin-coated plates. Sepharose 123-132 matrix metallopeptidase 9 Homo sapiens 4-9 22699735-3 2012 Here, we isolated a 75-kDa protein from E. coli on Sepharose column and identified it as ferric aerobactin receptor (IutA). Sepharose 51-60 Aerobactin siderophore ferric receptor protein IutA Escherichia coli 117-121 21830116-7 2012 We mixed beta-catenin mRNA with different amounts of spermine and performed electrophoresis on agarose gels, with a finding that the prior mixing greatly suppressed mRNA migration. Sepharose 96-103 catenin beta 1 L homeolog Xenopus laevis 10-22 22431046-7 2012 Agarose gel electrophoresis showed Adipo R2 was positively expressed in human periodontal ligament fibroblasts. Sepharose 0-7 adiponectin receptor 2 Homo sapiens 35-43 22156646-7 2012 RESULTS: The IEF on lab-prepared agarose gels was efficient since it was able to detect the main abnormal Hbs previously identified in the Tunisian population (HbS, HbC, HbO, and HbG). Sepharose 33-40 keratin 88, pseudogene Homo sapiens 165-168 22284455-0 2012 Electrochemiluminescence immunosensor based on graphene-CdS quantum dots-agarose composite for the ultrasensitive detection of alpha fetoprotein. Sepharose 73-80 CDP-diacylglycerol synthase 1 Homo sapiens 56-59 22284455-0 2012 Electrochemiluminescence immunosensor based on graphene-CdS quantum dots-agarose composite for the ultrasensitive detection of alpha fetoprotein. Sepharose 73-80 alpha fetoprotein Homo sapiens 127-144 22284455-3 2012 The novel G-CdS QDs-agarose composite was first coated on the glass carbon electrode surface to form a robust film, which exhibited high ECL intensity, good biocompatibility and high stability. Sepharose 20-27 CDP-diacylglycerol synthase 1 Homo sapiens 12-15 22284455-4 2012 After that 3-aminopropyl-triethoxysilane (APS), as a binding linker, was conjugated to the G-CdS QDs-agarose composite film on the electrode, the ECL signal was significantly enhanced. Sepharose 101-108 CDP-diacylglycerol synthase 1 Homo sapiens 93-96 22284455-8 2012 Besides, the highly enhanced ECL from the G-CdS QDs-agarose composite film opened new avenues to apply graphene and QDs ECL in analytical systems and ECL biosensors. Sepharose 52-59 CDP-diacylglycerol synthase 1 Homo sapiens 44-47 22297704-19 2012 It uses CaM-Sepharose beads to test proteins that bind to CaM and the influence of Ca(2+) on this binding. Sepharose 12-21 calmodulin 1 Homo sapiens 8-11 22297704-19 2012 It uses CaM-Sepharose beads to test proteins that bind to CaM and the influence of Ca(2+) on this binding. Sepharose 12-21 calmodulin 1 Homo sapiens 58-61 24956513-0 2012 Biomechanical Conditioning Enhanced Matrix Synthesis in Nucleus Pulposus Cells Cultured in Agarose Constructs with TGFbeta. Sepharose 91-98 transforming growth factor beta 1 Homo sapiens 115-122 22901138-2 2012 METHODS: Stool DNA was isolated and tumor-associated high molecular weight DNA (1.476 kb fragment including exons 6-9 of the p53 gene) was amplified using PCR and visualized on ethidium bromide-stained agarose gels. Sepharose 202-209 tumor protein p53 Homo sapiens 125-128 26316897-6 2012 Interesting agarose gel electrophoresis studies revealed that the nanoparticles efficiently complex with pCMV-Myc vector. Sepharose 12-19 MYC proto-oncogene, bHLH transcription factor Homo sapiens 110-113 22785483-4 2012 Purifying the Ang from the culture supernatant yielded 30 mg/L at 90% purity by chromatography with a SP Sepharose FF column. Sepharose 105-114 angiogenin Homo sapiens 14-17 22770475-10 2012 RT-PCR (reverse transcription-PCR), cell surface localization by immunofluorescence and enumeration by FACS analysis showed that the sphere cultures of U138MG grown on agarose-coated plates had elevated CD133 levels. Sepharose 168-175 prominin 1 Homo sapiens 203-208 23592932-6 2012 CAR messenger RNA was semiquantified by reverse transcription polymerase chain reaction followed by agarose gel analysis and measurement of band intensity. Sepharose 100-107 CXADR Ig-like cell adhesion molecule Homo sapiens 0-3 23119181-7 2012 Real-time qPCR and agarose gel electrophoresis revealed that the STC-1 amplicon generated from islet cell total RNA was the same size as that from kidney. Sepharose 19-26 stanniocalcin 1 Mus musculus 65-70 21930213-7 2012 A new modified method to purify G6PD, 6PGD, and GR, namely one chromatographic step using the 2",5"-ADP Sepharose 4B, is described for the first time in this study. Sepharose 104-116 glucose-6-phosphate dehydrogenase Rattus norvegicus 32-36 21930213-7 2012 A new modified method to purify G6PD, 6PGD, and GR, namely one chromatographic step using the 2",5"-ADP Sepharose 4B, is described for the first time in this study. Sepharose 104-116 glutathione-disulfide reductase Rattus norvegicus 48-50 22168155-3 2012 In this study, we determined effects of recombinant (r)SLURP-1 and-2 on migration of human epidermal and oral keratinocytes under agarose and epithelialization of cutaneous and oral mucosal excisional wounds in mice, and also identified nAChRs mediating SLURP signals. Sepharose 130-137 secreted LY6/PLAUR domain containing 1 Homo sapiens 55-68 22349390-4 2012 ICAM-1 sequences were amplified by PCRand analyzed by agarose gel electrophoresis. Sepharose 54-61 intercellular adhesion molecule 1 Homo sapiens 0-6 22071521-3 2011 On an avidin-agarose bead pull down assay, hnRNP A1 protein was selectively pulled down in the presence of C20-biotinylated CPT derivative (CPT-20-B) both in vitro and in vivo. Sepharose 13-20 heterogeneous nuclear ribonucleoprotein A1 Homo sapiens 43-51 22104730-6 2012 The MMP-9 production from monocytes was diminished by the depletion of fibronectin from the conditioned media with gelatin-Sepharose, and potentiated by culturing them in fibronectin-coated plates. Sepharose 123-132 fibronectin 1 Homo sapiens 71-82 21707870-8 2012 An IgG subtype-4 antibody was isolated from patient plasma using affinity chromatography on prothrombin-sepharose. Sepharose 104-113 coagulation factor II, thrombin Homo sapiens 92-103 22523468-5 2012 Ficolin B, pulled down using GlcNAc-agarose, contained very low, but detectable, amounts of MASP-2 and small MBL-associated protein (sMAP) and showed detectable C4-deposition activity on immobilized N-acetylglucosamine. Sepharose 36-43 ficolin B Mus musculus 0-9 22856523-6 2012 We demonstrate that 14-3-3gamma protein binds strongly to long DNA targets, as evidenced by electrophoretic mobility shift assay on agarose gels. Sepharose 132-139 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma Homo sapiens 20-31 22907375-5 2012 We report here a protocol for the purification of eukaryotically produced scFv TNF ligand fusion proteins based on affinity chromatography on anti-Flag agarose and further describe assays for the determination of the targeting index of this type of scFv-targeted proteins. Sepharose 152-159 tumor necrosis factor Homo sapiens 79-82 22808084-7 2012 Validation of the identified compounds employing the hemin-agarose affinity chromatography and mitochondrial transport assays demonstrated that three out of the four compounds were capable of inhibiting ABCB6 mediated hemin transport into isolated mitochondria. Sepharose 59-66 ATP binding cassette subfamily B member 6 (Langereis blood group) Homo sapiens 203-208 22121028-5 2012 In contrast, shifts in the mobility of various phosphorylated species of a high-molecular-mass protein, ataxia telangiectasia-mutated kinase (ATM, 350 kDa), could only be detected in the Tris-AcOH system with a 3% w/v polyacrylamide gel strengthened with 0.5% w/v agarose. Sepharose 264-271 ATM serine/threonine kinase Homo sapiens 104-140 22121028-5 2012 In contrast, shifts in the mobility of various phosphorylated species of a high-molecular-mass protein, ataxia telangiectasia-mutated kinase (ATM, 350 kDa), could only be detected in the Tris-AcOH system with a 3% w/v polyacrylamide gel strengthened with 0.5% w/v agarose. Sepharose 264-271 ATM serine/threonine kinase Homo sapiens 142-145 22017167-4 2011 RESULTS: A high-throughput system based upon agarose gel electrophoresis was developed and used to test 1040 drugs in a clinical drug library for their capacity to reduce cystatin C dimer formation in vitro. Sepharose 45-52 cystatin C Homo sapiens 171-181 21995946-1 2011 The cytosolic chaperonin TRiC was isolated from ovine testes using ultracentrifugation and heparin-Sepharose chromatography. Sepharose 99-108 MARVEL domain containing 2 Homo sapiens 25-29 22235674-5 2011 RESULTS: The recombinant plasmid pGEX-2T/HNE was successfully prepared and transferred into E. coli DH5o; the expression of the recombinant fusion protein GST/ HNE was successfully induced by IPTG at 18 degrees C overnight; and the purified recombinant protein HNE was successfully obtained by thrombin cleavage and purification of glutathione agarose beads. Sepharose 344-351 elastase, neutrophil expressed Homo sapiens 41-44 21870950-4 2011 At 0, 7, 14, and 21 days extracellular matrix (ECM) deposition within MSC-seeded agarose hydrogels due to transforming growth factor-beta3 stimulation was determined biochemically and histologically, and then reverse transcription-polymerase chain reaction was used to examine the effects of dynamic compression on cartilage-matrix-specific gene expression. Sepharose 81-88 transforming growth factor beta 3 Homo sapiens 106-138 22235674-5 2011 RESULTS: The recombinant plasmid pGEX-2T/HNE was successfully prepared and transferred into E. coli DH5o; the expression of the recombinant fusion protein GST/ HNE was successfully induced by IPTG at 18 degrees C overnight; and the purified recombinant protein HNE was successfully obtained by thrombin cleavage and purification of glutathione agarose beads. Sepharose 344-351 elastase, neutrophil expressed Homo sapiens 160-163 22235674-5 2011 RESULTS: The recombinant plasmid pGEX-2T/HNE was successfully prepared and transferred into E. coli DH5o; the expression of the recombinant fusion protein GST/ HNE was successfully induced by IPTG at 18 degrees C overnight; and the purified recombinant protein HNE was successfully obtained by thrombin cleavage and purification of glutathione agarose beads. Sepharose 344-351 elastase, neutrophil expressed Homo sapiens 160-163 22038803-2 2011 Approximately 86% electrophoretically and immunologically homogeneous SLE immunoglobulin Gs (IgGs) purified using several affinity resins including Sepharose with immobilized hMBP specifically hydrolyze only hMBP but not many other tested proteins. Sepharose 148-157 myelin basic protein Homo sapiens 208-212 21995315-5 2011 We show that chromatography on ConA-Sepharose can substitute for the immunoaffinity column and that bicelles can be used instead of detergent solution. Sepharose 36-45 CONA Bos taurus 31-35 21292017-0 2011 An agarose spot chemotaxis assay for chemokine receptor antagonists. Sepharose 3-10 C-X-C motif chemokine receptor 4 Homo sapiens 37-55 21292017-6 2011 In the presence of CXCL12, significant migration under the agarose spot is observed which can be retarded if a neutralising monoclonal antibody or a small molecule antagonist is added to the media. Sepharose 59-66 C-X-C motif chemokine ligand 12 Homo sapiens 19-25 21782027-0 2011 Human multiprotein bridging factor 1 and Calmodulin do not interact in vitro as confirmed by NMR spectroscopy and CaM-agarose affinity chromatography. Sepharose 118-125 calmodulin 1 Homo sapiens 114-117 21782027-3 2011 We applied NMR to investigate the interaction between the two proteins in solution and compared the results with those obtained with CaM-agarose affinity chromatography. Sepharose 137-144 calmodulin 1 Homo sapiens 133-136 21782027-6 2011 The discrepancy between present and previous experiments performed with CaM-agarose affinity chromatography depends on different experimental parameters suggesting that particular attention must be paid when CaM, or other immobilized proteins, are used to measure their affinity with putative partners. Sepharose 76-83 calmodulin 1 Homo sapiens 72-75 21782027-6 2011 The discrepancy between present and previous experiments performed with CaM-agarose affinity chromatography depends on different experimental parameters suggesting that particular attention must be paid when CaM, or other immobilized proteins, are used to measure their affinity with putative partners. Sepharose 76-83 calmodulin 1 Homo sapiens 208-211 22078448-3 2011 The fusion protein gene scFv-9R was then cloned into expression vector pQE30 and expressed in E.coli M15.Expressed protein was detected by SDS-PAGE and Western blot and purified by Ni-NTA chelating agarose. Sepharose 198-205 immunglobulin heavy chain variable region Homo sapiens 24-28 21852104-4 2011 The agarose bead sensors used in the device are sensitized with anti-C-reactive protein (CRP) antibody, and a fluorescent sandwich-type immunoassay was run to characterize the performance of this device. Sepharose 4-11 C-reactive protein Homo sapiens 69-87 21852104-4 2011 The agarose bead sensors used in the device are sensitized with anti-C-reactive protein (CRP) antibody, and a fluorescent sandwich-type immunoassay was run to characterize the performance of this device. Sepharose 4-11 C-reactive protein Homo sapiens 89-92 21962674-13 2011 The assays based on enzyme-entrapping agarose gels showed detection limits equal to 0.17 mug L(-1) and 0.29 mug L(-1) with immobilised PP2A from ZEU and PP1, respectively. Sepharose 38-45 protein phosphatase 2 phosphatase activator Homo sapiens 135-139 21962674-13 2011 The assays based on enzyme-entrapping agarose gels showed detection limits equal to 0.17 mug L(-1) and 0.29 mug L(-1) with immobilised PP2A from ZEU and PP1, respectively. Sepharose 38-45 inorganic pyrophosphatase 1 Homo sapiens 153-156 21853977-3 2011 Fibroblast growth factor-2 (FGF2) was immobilized within agarose hydrogels that were modified with two-photon labile 6-bromo-7-hydroxycoumarin-protected thiols. Sepharose 57-64 fibroblast growth factor 2 Homo sapiens 0-26 21853977-3 2011 Fibroblast growth factor-2 (FGF2) was immobilized within agarose hydrogels that were modified with two-photon labile 6-bromo-7-hydroxycoumarin-protected thiols. Sepharose 57-64 fibroblast growth factor 2 Homo sapiens 28-32 21925146-3 2011 Initially, P19 cells were attracted to CCN2-coated agarose beads. Sepharose 51-58 cellular communication network factor 2 Homo sapiens 39-43 21761116-6 2011 TGF-beta1 -509 C/T gene polymorphism was carried out by amplification refractory mutation system polymerase chain reaction (ARMS-PCR) method followed by agarose gel electrophoresis. Sepharose 153-160 transforming growth factor beta 1 Homo sapiens 0-9 21925787-6 2011 Under US exposure, the loaded uPA demonstrated bioactivity by agarose fibrin plate and in vitro thrombolysis of the three uPA-MBs also showed higher effects than in the group of those who received uPA-MBs alone, the control group or the US group. Sepharose 62-69 proline rich acidic protein 1 Homo sapiens 30-33 22379819-0 2011 [Effects of Schistosoma 22.6 kDa/26 GST molecule mixed with Sepharose 4B beads on induction of CD4+CD25+ regulatory T cells]. Sepharose 60-72 CD4 antigen Mus musculus 95-98 22379819-0 2011 [Effects of Schistosoma 22.6 kDa/26 GST molecule mixed with Sepharose 4B beads on induction of CD4+CD25+ regulatory T cells]. Sepharose 60-72 interleukin 2 receptor, alpha chain Mus musculus 99-103 22379819-9 2011 Meanwhile, the proportions of regulatory T cells were (17.0 +/- 80.57)% and (30.14 +/- 3.62)% when the CD4+ T cells were co-cultured with dendritic cells pulsed with Sepharose 4B beads mixed rSj22.6/26GST and OVA, respectively. Sepharose 166-178 CD4 antigen Mus musculus 103-106 21839207-7 2011 Considering that the immobilization step contributes to stabilize the PP2A activity, enzymes were entrapped within a photopolymer and an agarose gel. Sepharose 137-144 protein phosphatase 2 phosphatase activator Homo sapiens 70-74 21839207-10 2011 For instance, the IC(50) value obtained with the test based on PP2A from ZEU Immunotec immobilized within an agarose gel was 1.98 mug L(-1). Sepharose 109-116 protein phosphatase 2 phosphatase activator Homo sapiens 63-67 21912371-14 2011 Cell lysates are immunoprecipitated using an agarose-conjugated anti-myc antibody followed by western blot analysis. Sepharose 45-52 MYC proto-oncogene, bHLH transcription factor Homo sapiens 69-72 22338216-2 2011 METHODS: The genotypes of the gene DC-SIGN and DC-SIGNR in the pregnant women with HBV positive were detected by PCR and agarose gel electrophoresis. Sepharose 121-128 CD209 molecule Homo sapiens 35-42 22338216-2 2011 METHODS: The genotypes of the gene DC-SIGN and DC-SIGNR in the pregnant women with HBV positive were detected by PCR and agarose gel electrophoresis. Sepharose 121-128 C-type lectin domain family 4 member M Homo sapiens 47-55 21708156-6 2011 The ZnT8 variants were labeled with 35S-methionine and used in a standard RBA separating free from autoantibody-bound autoantigen with Protein A-Sepharose. Sepharose 145-154 solute carrier family 30 member 8 Homo sapiens 4-8 21499785-3 2011 The purification of a soluble AChE from sheep liver using affinity chromatography on Concanavalin A-Sepharose 4B and edrophonium-Sepharose 6B is studied. Sepharose 100-112 acetylcholinesterase Ovis aries 30-34 21683785-7 2011 On agarose gel electrophoresis, C3a comigrated with MDA-LDL and MAA-LDL, but not with native LDL or CuOx-LDL. Sepharose 3-10 complement C3 Homo sapiens 32-35 21707043-1 2011 We demonstrate that cytoskeletal actin-myosin networks can be encapsulated with high efficiency in giant liposomes by hydration of lipids in an agarose hydrogel. Sepharose 144-151 myosin heavy chain 14 Homo sapiens 39-45 21659008-0 2011 Direct electrochemistry of cytochrome c entrapped in agarose hydrogel in room temperature ionic liquids. Sepharose 53-60 cytochrome c, somatic Homo sapiens 27-39 21659008-1 2011 Direct electrochemistry of cytochrome c (cyt-c) entrapped in agarose hydrogel on gold electrode (Au), edge plane pyrolytic graphite electrode (EPPGE) and glassy carbon electrode (GC) in two room temperature ionic liquids was investigated. Sepharose 61-68 cytochrome c, somatic Homo sapiens 27-39 21659008-1 2011 Direct electrochemistry of cytochrome c (cyt-c) entrapped in agarose hydrogel on gold electrode (Au), edge plane pyrolytic graphite electrode (EPPGE) and glassy carbon electrode (GC) in two room temperature ionic liquids was investigated. Sepharose 61-68 cytochrome c, somatic Homo sapiens 41-46 21659008-2 2011 The effects of the addition of N,N-dimethylformamide (DMF) in the agarose-cyt-c film, water concentration in ionic liquids and exterior metal ions on the electrochemical behavior of cyt-c were monitored, and electrocatalytic properties of cyt-c were also done. Sepharose 66-73 cytochrome c, somatic Homo sapiens 74-79 21659008-3 2011 Results showed that a good quasi-reversible redox behavior of cyt-c could be found after adding DMF in agarose-cyt-c film, and peak shape would not change after continuously scanning for 50 cycles. Sepharose 103-110 cytochrome c, somatic Homo sapiens 62-67 21659008-3 2011 Results showed that a good quasi-reversible redox behavior of cyt-c could be found after adding DMF in agarose-cyt-c film, and peak shape would not change after continuously scanning for 50 cycles. Sepharose 103-110 cytochrome c, somatic Homo sapiens 111-116 21913551-2 2011 METHODS: RT-PCR products of HSP70 mRNA were tested by agarose gel electrophoresis after RT-PCR amplification. Sepharose 54-61 heat shock protein family A (Hsp70) member 1B Rattus norvegicus 28-33 21659008-6 2011 Interestingly, cyt-c entrapped in agarose hydrogel on EPPGE and GC could catalyze the electroreduction of trichloroacetic acid (TCA) and tert-butyl hydroperoxide (t-BuOOH) in [Bmim][BF(4)], but could not in [Bmim][Br]. Sepharose 34-41 cytochrome c, somatic Homo sapiens 15-20 20452068-8 2011 A marked induction in caspase-3 and Na(+)-K(+) ATPase levels and DNA laddering as revealed by agarose gel electrophoresis was observed in rat myocardium of pathological group. Sepharose 94-101 caspase 3 Rattus norvegicus 22-53 21867609-7 2011 Agarose gel electrophoresis of DNA from K562 treated with Tcd A revealed a "ladder" pattern. Sepharose 0-7 CHM Rab escort protein Homo sapiens 58-61 21755988-4 2011 All of the activating activity and 20-30% of the Hsp70 elute in the flow-through fraction upon subsequent ATP-agarose chromatography. Sepharose 110-117 heat shock protein family A (Hsp70) member 4 Homo sapiens 49-54 21755988-7 2011 Further fractionation of the ATP-agarose flow-through on Sephacryl S-300 separates free thioredoxin from apo-nNOS activating activity, Hsp70, and a small amount of thioredoxin, all of which are eluted throughout the macromolecular peak. Sepharose 33-40 thioredoxin Homo sapiens 88-99 21707360-12 2011 The scFv protein from IPTG-induced cells was purified under native conditions by immobilized metal affinity chromatography on a Ni-NTA agarose column. Sepharose 135-142 immunglobulin heavy chain variable region Homo sapiens 4-8 21600887-10 2011 In agreement with efficient AT1/B2R heterodimerization, confocal FRET imaging of co-enriched receptor proteins immobilized on agarose beads also detected a high FRET efficiency of 24.0%. Sepharose 126-133 angiotensin II receptor type 1 Homo sapiens 28-31 21482826-2 2011 We have previously demonstrated by affinity chromatography that HIV-1 p17 binds strongly to heparin-agarose at physiological pH and to human activated CD4(+) T cells. Sepharose 100-107 family with sequence similarity 72 member B Homo sapiens 70-73 21657982-2 2011 Applying SDS-homogeneous DP 4 onto an analytical agarose isoelectric focusing (IEF) gel, pH 4-6, activity staining resulted in at least 17 isoforms between pH 4.8-6.0. Sepharose 49-56 transcription factor Dp family member 3 Homo sapiens 25-29 21681347-12 2011 The results showed that melting curves exhibited sharp peaks, and PCR product also generated prominent band with expected size in agarose gel electrophoresis, which validated the optimization of the selected primer sets of IL-1beta and Caspase-3 genes. Sepharose 130-137 interleukin 1 beta Rattus norvegicus 223-231 21681347-12 2011 The results showed that melting curves exhibited sharp peaks, and PCR product also generated prominent band with expected size in agarose gel electrophoresis, which validated the optimization of the selected primer sets of IL-1beta and Caspase-3 genes. Sepharose 130-137 caspase 3 Rattus norvegicus 236-245 21636552-3 2011 EXPERIMENTAL DESIGN: The constitutively active BRAF(V600E) allele was introduced into human neurospheres, and its effects on MAPK (mitogen-activated protein kinase) signaling, proliferation, soft agarose colony formation, stem cell phenotype, and induction of cellular senescence were assayed. Sepharose 196-203 B-Raf proto-oncogene, serine/threonine kinase Homo sapiens 47-51 21636552-10 2011 CONCLUSIONS: BRAF activation in human neural stem and progenitor cells initially promotes clonogenic growth in soft agarose, suggesting partial cellular transformation, but oncogene-induced senescence subsequently limits proliferation. Sepharose 116-123 B-Raf proto-oncogene, serine/threonine kinase Homo sapiens 13-17 21647922-8 2011 In addition, our method has the additional advantage of being able to resolve the triplet structure of platelet vWF multimers, which has not been identified previously through conventional SDS-agarose electrophoresis multimer analysis. Sepharose 193-200 von Willebrand factor Homo sapiens 112-115 21086172-5 2011 SLAP was extracted and purified from LP cells using a porcine stomach mucin-Sepharose 4B column. Sepharose 76-85 Src like adaptor Homo sapiens 0-4 21393420-3 2011 Initial studies in vitro, using the under-agarose gel assay, determined that GM-CSF can induce neutrophil migration at a much lower molar concentration than the fMLP-like peptide WKYMVm (33.5-134 nM vs. 1-10 muM). Sepharose 42-49 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 77-83 21651865-4 2011 The recombinant hCRT was purified through Ni(2+) -NT agarose gel column and the purified hCRT used as immunogen to immunize the rabbit.The titer and specificity of the rabbit anti-hCRT antibody were analyzed by ELISA, Western blot and immunohistochemical staining, respectively. Sepharose 53-60 calreticulin Homo sapiens 16-20 20831652-5 2011 MTHFR genotypes were determined by polymerase chain reaction and by Hindf I restriction enzyme analysis and subsequent 3% agarose gel electrophoresis techniques. Sepharose 122-129 methylenetetrahydrofolate reductase Homo sapiens 0-5 21418450-5 2011 The soluble HB-EGF in culture media was measured by heparin agarose chromatography/Western blot analysis. Sepharose 60-67 heparin binding EGF like growth factor Homo sapiens 12-18 21504585-5 2011 FINDINGS: PCR amplification using CCK2R specific primer-pairs, followed by ethidium-bromide stained agarose gel electrophoresis revealed the expression of wild-type CCK2R mRNA in 12 of 17 biopsy specimens. Sepharose 100-107 cholecystokinin B receptor Homo sapiens 165-170 21411102-8 2011 Additionally, both analytical and numerical predictions compare favorably with experimental column breakthrough data for lysozyme-cytochrome c mixtures on the strong cation exchanger SP Sepharose FF. Sepharose 186-195 cytochrome c, somatic Homo sapiens 130-142 21434664-5 2011 For the metabolism of chlortoluron, a selective herbicide used to control grass weeds, CYP1A1 immobilized in agarose gel showed a higher activity and stability compared with those in silica gels and free suspensions. Sepharose 109-116 cytochrome P450 family 1 subfamily A member 1 Homo sapiens 87-93 21222489-7 2011 We utilized an excess of immobilized calmodulin on agarose beads and formed complexes with varying quantities of mastoparan and melittin. Sepharose 51-58 calmodulin 1 Homo sapiens 37-47 21526145-5 2011 Analytical agarose gel electrophoresis (AAGE) studies showed that TRF2 promoted the folding of nucleosomal arrays into more compact structures by neutralizing negative surface charge. Sepharose 11-18 telomeric repeat binding factor 2 Homo sapiens 66-70 21492414-8 2011 Cross-talk between C/EBPdelta and NF-kappaB was investigated by analyzing binding to a kappaB site using a biotin streptavidin-agarose pull-down assay. Sepharose 127-134 CCAAT/enhancer binding protein (C/EBP), delta Mus musculus 19-29 21492414-8 2011 Cross-talk between C/EBPdelta and NF-kappaB was investigated by analyzing binding to a kappaB site using a biotin streptavidin-agarose pull-down assay. Sepharose 127-134 nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 Mus musculus 34-43 21574063-4 2011 After purification with a Q Sepharose Fast Flow column, a natural N-terminal TNFR1 PLAD which purity was up to 95%, was obtained and was identified using Nano LC-ECI-MS/MS. Sepharose 28-37 TNF receptor superfamily member 1A Homo sapiens 77-82 21165474-3 2011 GBP-Badan was attached via an oligohistidine-tag to the surface of Ni-nitrilotriacetic acid (NTA)-functionalized agarose or polystyrene beads. Sepharose 113-120 transmembrane protein 132A Homo sapiens 0-3 21566867-4 2011 The polymorphism of TNF-alpha-308 genotype (TNF1/2) was examined after electrophoresis on agarose gel and ethidium bromide staining. Sepharose 90-97 tumor necrosis factor Homo sapiens 20-29 21477733-5 2011 The ACE fragment containing the polymorphism was amplified using conventional polymerase chain reaction using specific primer pairs and subsequently genotyped using agarose gel electrophoresis. Sepharose 165-172 angiotensin I converting enzyme Homo sapiens 4-7 21245481-4 2011 Anti-beta(2)-GP1 autoantibodies from 3 patients with antiphospholipid syndrome were affinity-purified using human beta(2)-GP1 bound to agarose. Sepharose 135-142 GTP binding protein 1 Homo sapiens 122-125 21476192-3 2011 Polymorphisms of the ERbeta gene and p53 were assessed by PCR and analyzed on 2% agarose gel stained with ethidium bromide. Sepharose 81-88 estrogen receptor 2 Homo sapiens 21-27 21046196-8 2011 Repeated elution of protein from CM-cellulose and Co-agarose increased the yield of rOSM to 200 mg from 2 l culture. Sepharose 53-60 oncostatin M Rattus norvegicus 84-88 22159485-5 2011 MATERIALS AND METHODS: Protein disulfide isomerase was isolated from a bovine liver homogenate using anti-PDI antibodies coupled to agarose through hydrazone bonds. Sepharose 132-139 prolyl 4-hydroxylase subunit beta Bos taurus 23-50 21159379-4 2011 Using this device, we demonstrated that (1) agarose-alginate mixture can be gelled thermally, thus an excellent candidate for forming multi-layered scaffolds for micropatterning embedded cells; (2) primary cortical neurons were cultured successfully for up to three weeks in the micropatterned multi-layered scaffold; (3) B27 concentration gradient enhanced neurite outgrowth. Sepharose 44-51 melanocortin 2 receptor accessory protein Homo sapiens 322-325 22159485-5 2011 MATERIALS AND METHODS: Protein disulfide isomerase was isolated from a bovine liver homogenate using anti-PDI antibodies coupled to agarose through hydrazone bonds. Sepharose 132-139 prolyl 4-hydroxylase subunit beta Bos taurus 106-109 22303054-9 2011 According to agarose gel electrophoresis, full complexation was obtained at 0.1/1 and higher chitosan/pGM-CSF ratios. Sepharose 13-20 colony stimulating factor 2 Homo sapiens 106-109 21418861-8 2011 RESULTS: The length of c-kit shRNA was around 50 bp in agarose electrophoresis. Sepharose 55-62 KIT proto-oncogene receptor tyrosine kinase Mus musculus 23-28 20828618-7 2011 Active human DPPI was purified with a three-step purification strategy employing: Butyl Sepharose 4 Fast Flow, Sephadex G-25 Medium and Q Sepharose Fast Flow chromatography. Sepharose 88-97 cathepsin C Homo sapiens 13-17 21485192-2 2011 The bFGF loading agarose-grafting hyaluronan scaffold had homogenous porosities, and the loaded bFGF was bioactive in 2 weeks. Sepharose 17-24 fibroblast growth factor 2 Mus musculus 4-8 20722637-15 2011 Specific IgE was demonstrated by Sepharose-RIA and inhibition assay. Sepharose 33-42 immunoglobulin heavy constant epsilon Homo sapiens 9-12 21485192-2 2011 The bFGF loading agarose-grafting hyaluronan scaffold had homogenous porosities, and the loaded bFGF was bioactive in 2 weeks. Sepharose 17-24 fibroblast growth factor 2 Mus musculus 96-100 21368419-6 2011 RESULTS: The ApoO gene was cloned by PCR and a 519 bp DNA fragment was shown on the agarose electrophoresis. Sepharose 84-91 apolipoprotein O Homo sapiens 13-17 21418831-4 2011 The promoter and all eighteen exons of LDLR gene were investigated by using PCR and agarose gel electrophoresis in combination with DNA sequence analysis. Sepharose 84-91 low density lipoprotein receptor Homo sapiens 39-43 21115479-6 2011 GST-Ric-8 Galpha complexes were isolated from whole cell detergent lysates with glutathione-Sepharose. Sepharose 92-101 RIC8 guanine nucleotide exchange factor A Homo sapiens 4-9 21115479-6 2011 GST-Ric-8 Galpha complexes were isolated from whole cell detergent lysates with glutathione-Sepharose. Sepharose 92-101 succinate-CoA ligase GDP/ADP-forming subunit alpha Homo sapiens 10-16 20969915-3 2011 Phosvitin was immobilized on agarose beads with divinyl sulfone. Sepharose 29-36 casein kinase 2 beta Homo sapiens 0-9 20969915-4 2011 Five aliquots of phosvitin-immobilized agarose beads were incubated in control or experimental mineralizing solution. Sepharose 39-46 casein kinase 2 beta Homo sapiens 17-26 20969915-8 2011 RESULTS: Phosvitin-immobilized agarose beads induced mineral formation after incubation for 5.3h in the metastable solution without ions eluted from S-PRG fillers. Sepharose 31-38 casein kinase 2 beta Homo sapiens 9-18 21528449-4 2011 We developed a native agarose gel system to directly detect Ago1-RISC, Ago2-RISC, and their precursor complexes. Sepharose 22-29 Argonaute-1 Drosophila melanogaster 60-64 21528449-4 2011 We developed a native agarose gel system to directly detect Ago1-RISC, Ago2-RISC, and their precursor complexes. Sepharose 22-29 Argonaute 2 Drosophila melanogaster 71-75 21914170-0 2011 Biomechanical signals and the C-type natriuretic peptide counteract catabolic activities induced by IL-1beta in chondrocyte/agarose constructs. Sepharose 124-131 natriuretic peptide C Homo sapiens 30-56 21914170-0 2011 Biomechanical signals and the C-type natriuretic peptide counteract catabolic activities induced by IL-1beta in chondrocyte/agarose constructs. Sepharose 124-131 interleukin 1 beta Homo sapiens 100-108 21914170-1 2011 INTRODUCTION: The present study examined the effect of C-type natriuretic peptide (CNP) on the anabolic and catabolic activities in chondrocyte/agarose constructs subjected to dynamic compression. Sepharose 144-151 natriuretic peptide C Homo sapiens 55-81 21055395-4 2011 In this study affinity chromatography on MPO-Sepharose showed that MPO-LDL complexes are uncoupled at ionic strength above 0.3M NaCl or when pH of solution goes below 3.6. Sepharose 45-54 myeloperoxidase Homo sapiens 41-44 21055395-4 2011 In this study affinity chromatography on MPO-Sepharose showed that MPO-LDL complexes are uncoupled at ionic strength above 0.3M NaCl or when pH of solution goes below 3.6. Sepharose 45-54 myeloperoxidase Homo sapiens 67-70 21250880-4 2011 alpha-Globin molecular analysis was performed by polymerase chain reaction (PCR) followed by agarose gel electrophoresis, reverse hybridization test strips or DNA sequencing. Sepharose 93-100 hemoglobin subunit alpha 2 Homo sapiens 0-12 21383853-6 2011 The efficiency of absorbing DNA and resisting deoxyribonuclease I (DNase I) digestion when bound to Fe(3)O(4)-dextran-anti-beta-HCG nanoparticles was examined by agarose gel electrophoresis. Sepharose 162-169 deoxyribonuclease 1 Homo sapiens 46-65 21276381-7 2011 Human leukocyte antigen DRB1 type was determined by agarose gel electrophoresis and results were recorded. Sepharose 52-59 major histocompatibility complex, class II, DR beta 1 Homo sapiens 24-28 20978971-2 2011 MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure. Sepharose 64-71 myelin basic protein Homo sapiens 0-3 21857970-6 2011 The in vitro streptavidin-agarose pulldown assay and in vivo chromatin immunoprecipitation assay showed that quercetin considerably inhibited the binding of the transactivators CREB2, C-Jun, C/EBPbeta and NF-kappaB and blocked the recruitment of the coactivator p300 to COX-2 promoter. Sepharose 26-33 activating transcription factor 2 Homo sapiens 177-182 20672992-5 2011 In parallel experiments, TGF-beta1 adsorbed to agarose hydrogels stimulated comparable chondrogenesis. Sepharose 47-54 transforming growth factor beta 1 Homo sapiens 25-34 20672992-6 2011 Full-length aggrecan was produced by BMSCs in response to Ads-TGF in both peptide and agarose hydrogels, whereas medium-delivered TGF-beta1 stimulated catabolic aggrecan cleavage product formation in agarose but not peptide scaffolds. Sepharose 200-207 transforming growth factor beta 1 Homo sapiens 130-139 20869111-1 2010 Previous studies reported that matrix protein supplementation (fibronectin/fibrinogen, FN/FG) of agarose gel microcapsules enhances survival and target tissue retention of syngeneic rat marrow stromal cells (MSCs). Sepharose 97-104 fibronectin 1 Rattus norvegicus 63-74 21208560-5 2011 RESULTS: Agarose gel electrophoresis detection showed that human Gax DNA segment was about 915 bp, which accorded with the expectation. Sepharose 9-16 mesenchyme homeobox 2 Homo sapiens 65-68 21144000-7 2010 Also, we confirm CHS-828 and TP201565 as competitive inhibitors of NAMPT through docking studies and by NAMPT precipitation from cellular lysate by an analogue of TP201565 linked to sepharose. Sepharose 182-191 nicotinamide phosphoribosyltransferase Homo sapiens 67-72 20861465-8 2010 The pro-angiogenic effect of ANG II can be attributed to an enhanced endothelial cell migration because both transwell and under agarose migration assays revealed a 37% and 101% increase in cell motility, respectively. Sepharose 129-136 angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Mus musculus 29-35 21191765-5 2010 Binding of salaceyins to human Hsp90alpha was examined by competition binding experiments with ATP-Sepharose beads. Sepharose 99-108 heat shock protein 90 alpha family class A member 1 Homo sapiens 31-41 20723574-2 2010 In this study, a basic myotoxic PLA(2), named EcTx-I was isolated from Echis carinatus snake venom by using gel filtration on Superdex G-75, and reverse phase HPLC on C18 and C8 Sepharose columns. Sepharose 178-187 phospholipase A2 group IIA Homo sapiens 32-38 21098716-6 2010 The ability to self-renew was confirmed by the observation that ALDH+CD44+Lin- cells sorted from human HNSCC formed more spheroids (orospheres) in 3-D agarose matrices or ultra-low attachment plates than controls and were serially passaged in vivo. Sepharose 151-158 CD44 molecule (Indian blood group) Homo sapiens 69-73 21041096-2 2010 Agarose gel and precipitation assays indicate their improved inhibitory activity on ribonuclease A (RNase A). Sepharose 0-7 ribonuclease A family member 1, pancreatic Homo sapiens 84-98 21041096-2 2010 Agarose gel and precipitation assays indicate their improved inhibitory activity on ribonuclease A (RNase A). Sepharose 0-7 ribonuclease A family member 1, pancreatic Homo sapiens 100-107 20937523-4 2010 The presence of high affinity salmon heparin was demonstrated by using chromatography on antithrombin-Sepharose. Sepharose 102-111 antithrombin protein Salmo salar 89-101 21311674-6 2010 Direct interaction of magnolol and p38 was further confirmed by pull down assay using magnolol conjugated to Sepharose 4B beads. Sepharose 109-121 mitogen-activated protein kinase 14 Mus musculus 35-38 21142919-3 2010 Genotyping methods for the TYMS 5"-UTR polymorphisms have typically involved visualizing PCR and RFLP products on agarose gels. Sepharose 114-121 thymidylate synthetase Homo sapiens 27-31 21431266-4 2010 Finally, the recombinant plasmid pBABE-hygro-PLAP-1 was confirmed by agarose gel electrophoresis and DNA sequence analysis. Sepharose 69-76 asporin Homo sapiens 45-51 20889192-2 2010 To improve the sensitivity, precision and efficiency of this assay, we developed and validated a new in-gel infrared fluorescent VWF multimer imaging method to visualize and quantify VWF multimers directly in the agarose gel, thus eliminating electroblotting or autoradiographic steps. Sepharose 213-220 von Willebrand factor Homo sapiens 129-132 20889192-2 2010 To improve the sensitivity, precision and efficiency of this assay, we developed and validated a new in-gel infrared fluorescent VWF multimer imaging method to visualize and quantify VWF multimers directly in the agarose gel, thus eliminating electroblotting or autoradiographic steps. Sepharose 213-220 von Willebrand factor Homo sapiens 183-186 21138692-3 2010 The recombinant GST/SREBP1 was expressed in E.coli with IPTG induction and purified by Glutathione Sepharose 4B affinity chromatography. Sepharose 99-111 sterol regulatory element binding transcription factor 1 Gallus gallus 20-26 21176335-3 2010 The results showed that the flt3-LM was detected in 20.2% (20/99) patients by agarose gel electrophoresis, and in 29.9% (29/99) by denaturing PAGE. Sepharose 78-85 fms related receptor tyrosine kinase 3 Homo sapiens 28-32 20823265-5 2010 First, FLVCR2 binds to hemin-conjugated agarose, and binding is competed by free hemin. Sepharose 40-47 FLVCR heme transporter 2 Homo sapiens 7-13 20966350-3 2010 Immunohistochemical analysis, the pull-down assay using Klotho-fixed agarose, and FRET confocal imaging confirmed that Klotho protein binds directly to VEGF receptor 2 (VEGFR-2) and endothelial, transient-receptor potential canonical Ca(2+) channel 1 (TRPC-1) and strengthens the association to promote their cointernalization. Sepharose 69-76 klotho Mus musculus 119-125 20966350-3 2010 Immunohistochemical analysis, the pull-down assay using Klotho-fixed agarose, and FRET confocal imaging confirmed that Klotho protein binds directly to VEGF receptor 2 (VEGFR-2) and endothelial, transient-receptor potential canonical Ca(2+) channel 1 (TRPC-1) and strengthens the association to promote their cointernalization. Sepharose 69-76 kinase insert domain protein receptor Mus musculus 152-167 20108052-1 2010 Lectin has been isolated from mycelia of Aspergillus terricola by single step purification on porcine stomach mucin-Sepharose 4B affinity column. Sepharose 116-125 mucin 1, cell surface associated Bos taurus 110-115 20684984-0 2010 The use of vascular endothelial growth factor functionalized agarose to guide pluripotent stem cell aggregates toward blood progenitor cells. Sepharose 61-68 vascular endothelial growth factor A Mus musculus 11-45 21137981-2 2010 PON1 was purified from human serum using ammonium sulfate precipitation and hydrophobic interaction chromatography (Sepharose 4B, L-tyrosine, 1-Napthylamine) and magnetic iron oxide nanoparticles were prepared by co-precipitation Fe(+2) and Fe(+3) ions in an ammonia solution at room temperature. Sepharose 116-128 paraoxonase 1 Homo sapiens 0-4 20861221-5 2010 Binding of the noncovalent complex to Sepharose-coupled AMHRII induces dissociation of the pro-region from the mature C-terminal dimer, whereas no dissociation occurs after binding to immobilized AMH antibodies. Sepharose 38-47 anti-Mullerian hormone Homo sapiens 56-59 21097416-6 2010 The recombinant pSG5/TRIF was extracted, purified, and identified by restriction endonuclease BamH I and agarose gel electrophoresis. Sepharose 105-112 pregnancy specific beta-1-glycoprotein 5 Homo sapiens 16-20 21097416-6 2010 The recombinant pSG5/TRIF was extracted, purified, and identified by restriction endonuclease BamH I and agarose gel electrophoresis. Sepharose 105-112 TIR domain containing adaptor molecule 1 Homo sapiens 21-25 20859638-6 2010 Recombinant TcCYS3 and TcCYS4 immobilized in CNBr-Sepharose were efficient to capture M. perniciosa proteases from culture media. Sepharose 50-59 cysteine proteinase inhibitor 7 Theobroma cacao 12-18 20859638-6 2010 Recombinant TcCYS3 and TcCYS4 immobilized in CNBr-Sepharose were efficient to capture M. perniciosa proteases from culture media. Sepharose 50-59 cysteine proteinase inhibitor 6 Theobroma cacao 23-29 21055351-4 2010 RESULTS: NKG2D, perforin and granzyme B mRNA could be specifically amplified and quantitatively detected by the SYBR Green I real-time fluorescence quantitative PCR according to agarose gel electrophoresis and melt curve analysis. Sepharose 178-185 killer cell lectin like receptor K1 Homo sapiens 9-14 21055351-4 2010 RESULTS: NKG2D, perforin and granzyme B mRNA could be specifically amplified and quantitatively detected by the SYBR Green I real-time fluorescence quantitative PCR according to agarose gel electrophoresis and melt curve analysis. Sepharose 178-185 granzyme B Homo sapiens 29-39 21081038-6 2010 The GST-TLE1-Q(1-136) fusion protein was induced by IPTG, digested by Thrombin, purified with glutathione-sepharose beads and FPLC, identified by SDS-PAGE. Sepharose 106-115 glutathione S-transferase kappa 1 Homo sapiens 4-7 20931600-1 2010 The mass transfer and adsorption kinetics of self-associating apolipoprotein A-I(Milano) (apoA-I(M)) was investigated for the two anion exchangers Q-Sepharose-HP and Macro-Prep-HQ. Sepharose 149-158 apolipoprotein A1 Homo sapiens 62-88 21081038-6 2010 The GST-TLE1-Q(1-136) fusion protein was induced by IPTG, digested by Thrombin, purified with glutathione-sepharose beads and FPLC, identified by SDS-PAGE. Sepharose 106-115 TLE family member 1, transcriptional corepressor Homo sapiens 8-12 20976851-8 2010 RESULTS: The length of KIT shRNA was determined to be about 50bp by agarose electrophoresis. Sepharose 68-75 KIT proto-oncogene receptor tyrosine kinase Mus musculus 23-26 20942929-6 2010 3- RNAi directed against serpinE2 in caMEK-transformed rat IECs or in human CRC cell lines HCT116 and LoVo markedly decreased foci formation, anchorage-independent growth in soft agarose, cell migration and tumor formation in nude mice. Sepharose 179-186 serpin family E member 2 Homo sapiens 25-33 20392170-3 2010 SIRT1 formed mixed disulfides with GSNO-Sepharose, and mass spectrometry identified several cysteines that are modified by GSNO, including Cys-67 which was S-glutathiolated. Sepharose 40-49 sirtuin 1 Homo sapiens 0-5 19823931-6 2010 In addition, we have also identified several ER resident proteins including molecular chaperones like ORP150, Hsp70, Grp78, BiP of A431 cells, which were bound to the Jacalin-sepharose column. Sepharose 175-184 hypoxia up-regulated 1 Homo sapiens 102-108 19823931-6 2010 In addition, we have also identified several ER resident proteins including molecular chaperones like ORP150, Hsp70, Grp78, BiP of A431 cells, which were bound to the Jacalin-sepharose column. Sepharose 175-184 heat shock protein family A (Hsp70) member 4 Homo sapiens 110-115 19823931-6 2010 In addition, we have also identified several ER resident proteins including molecular chaperones like ORP150, Hsp70, Grp78, BiP of A431 cells, which were bound to the Jacalin-sepharose column. Sepharose 175-184 heat shock protein family A (Hsp70) member 5 Homo sapiens 117-122 19823931-6 2010 In addition, we have also identified several ER resident proteins including molecular chaperones like ORP150, Hsp70, Grp78, BiP of A431 cells, which were bound to the Jacalin-sepharose column. Sepharose 175-184 heat shock protein family A (Hsp70) member 5 Homo sapiens 124-127 20668163-8 2010 KHDC1A and 1B bind polyU agarose and form oligomers like other KH-domain proteins. Sepharose 25-32 KH domain containing 1A Mus musculus 0-13 20457256-12 2010 Purification of eGFP from the gene fusion product, SUMO2-ubiquitin-eGFP, involved cleavage by a deubiquitinase (Usp2-cc) and Ni-Sepharose column chromatography. Sepharose 128-137 small ubiquitin like modifier 2 Homo sapiens 51-56 20886107-3 2010 PRINCIPAL FINDINGS: Human blood serum was passed over Sepharose conjugated with CS-A, and CS-A-specific binding proteins were identified by Western blotting and mass spectrometric analysis. Sepharose 54-63 chorionic somatomammotropin hormone 1 Homo sapiens 80-84 20538449-2 2010 The plant beta(1 3)-D-glucanase (betaG), glucose oxidase (GOD) or/and peroxidase (POD) in agarose-corn flour-gelatin (ACG) matrix were coated on platinum disc electrode to detect soluble beta(1 3)-D-glucan. Sepharose 90-97 peroxidase 1 Zea mays 70-80 20538449-2 2010 The plant beta(1 3)-D-glucanase (betaG), glucose oxidase (GOD) or/and peroxidase (POD) in agarose-corn flour-gelatin (ACG) matrix were coated on platinum disc electrode to detect soluble beta(1 3)-D-glucan. Sepharose 90-97 peroxidase 1 Zea mays 82-85 20680969-2 2010 The starch-agarose gel electrophoresis of hemolysate, RBCs, freeze-thawed RBCs and the supernatant of freeze-thawed RBCs showed that the interaction between HbA and HbA(2) was affected by membrane integrity. Sepharose 11-18 keratin 90, pseudogene Homo sapiens 157-160 20826309-4 2010 Immobilization of a potent GSM onto an agarose matrix quantitatively recovered Pen-2 and to a lesser degree PS-1 NTFs from cellular extracts. Sepharose 39-46 presenilin enhancer gamma secretase subunit Mus musculus 79-84 20569198-9 2010 Upon incubation of IRP-1 with increasing concentrations of ATP or ADP, the protein migrates more slowly on agarose gel electrophoresis, and there is a shift in the CD spectrum. Sepharose 107-114 aconitase 1 Homo sapiens 19-24 20680969-2 2010 The starch-agarose gel electrophoresis of hemolysate, RBCs, freeze-thawed RBCs and the supernatant of freeze-thawed RBCs showed that the interaction between HbA and HbA(2) was affected by membrane integrity. Sepharose 11-18 keratin 90, pseudogene Homo sapiens 165-168 20680969-3 2010 To identify the proteins involved in the interaction, protein components located between HbA and HbA(2) in RBCs (RBC HbA-HbA(2)) and hemolysate (hemolysate HbA-HbA(2)) were isolated from the starch-agarose gel and separated by 5-12% SDS-PAGE. Sepharose 198-205 keratin 90, pseudogene Homo sapiens 89-92 20680969-3 2010 To identify the proteins involved in the interaction, protein components located between HbA and HbA(2) in RBCs (RBC HbA-HbA(2)) and hemolysate (hemolysate HbA-HbA(2)) were isolated from the starch-agarose gel and separated by 5-12% SDS-PAGE. Sepharose 198-205 keratin 90, pseudogene Homo sapiens 97-100 20680969-3 2010 To identify the proteins involved in the interaction, protein components located between HbA and HbA(2) in RBCs (RBC HbA-HbA(2)) and hemolysate (hemolysate HbA-HbA(2)) were isolated from the starch-agarose gel and separated by 5-12% SDS-PAGE. Sepharose 198-205 keratin 90, pseudogene Homo sapiens 97-100 20680969-3 2010 To identify the proteins involved in the interaction, protein components located between HbA and HbA(2) in RBCs (RBC HbA-HbA(2)) and hemolysate (hemolysate HbA-HbA(2)) were isolated from the starch-agarose gel and separated by 5-12% SDS-PAGE. Sepharose 198-205 keratin 90, pseudogene Homo sapiens 97-100 20680969-3 2010 To identify the proteins involved in the interaction, protein components located between HbA and HbA(2) in RBCs (RBC HbA-HbA(2)) and hemolysate (hemolysate HbA-HbA(2)) were isolated from the starch-agarose gel and separated by 5-12% SDS-PAGE. Sepharose 198-205 keratin 90, pseudogene Homo sapiens 97-100 20680969-3 2010 To identify the proteins involved in the interaction, protein components located between HbA and HbA(2) in RBCs (RBC HbA-HbA(2)) and hemolysate (hemolysate HbA-HbA(2)) were isolated from the starch-agarose gel and separated by 5-12% SDS-PAGE. Sepharose 198-205 keratin 90, pseudogene Homo sapiens 97-100 20583146-6 2010 Anti-histone IgGs were purified by the affinity chromatography on histone H1-Sepharose. Sepharose 77-86 H1.0 linker histone Homo sapiens 66-76 20957167-6 2010 Agarose gel electrophoresis of the nanoparticles with the two different N/P ratios showed that these nanoparticles could protect EGFR antisense molecules for six hours. Sepharose 0-7 epidermal growth factor receptor Homo sapiens 129-133 20603150-7 2010 We have immobilised Fe(3+) to an inert Sepharose resin; this resin was capable of quantitatively removing endogenous and recombinant PrP(C) and recombinant beta PrP from complex solutions. Sepharose 39-48 prion protein Homo sapiens 133-136 20603150-7 2010 We have immobilised Fe(3+) to an inert Sepharose resin; this resin was capable of quantitatively removing endogenous and recombinant PrP(C) and recombinant beta PrP from complex solutions. Sepharose 39-48 prion protein Homo sapiens 161-164 20681675-3 2010 The protein samples pretreated with 130 mM dithiothreitol before the step of CM-Sepharose chromatography could greatly enhance the separation of the targeted beta-glucosidase from the impurities. Sepharose 80-89 LOC547491 Glycine max 158-174 20797316-4 2010 The ATX-Fc fusion protein was expressed in HEK293 cells and isolated from conditioned medium of a stable clone by affinity chromatography with Protein A sepharose followed by cleavage with thrombin. Sepharose 153-162 ectonucleotide pyrophosphatase/phosphodiesterase 2 Homo sapiens 4-7 20554434-7 2010 Agarose beads containing EPO were implanted into dorsal skin of C57BL/6 mice to examine effects of EPO on hair growth in vivo. Sepharose 0-7 erythropoietin Mus musculus 25-28 20891039-2 2010 Concurrently, sepharose column chromatography revealed an association of the bulk of plasma CRP with very low-density lipoproteins. Sepharose 14-23 C-reactive protein Homo sapiens 92-95 20451568-5 2010 The soluble thioredoxin/TbetaRII-ED fusion has been purified and refolded on Ni-NTA agarose. Sepharose 84-91 thioredoxin Homo sapiens 12-23 20451568-5 2010 The soluble thioredoxin/TbetaRII-ED fusion has been purified and refolded on Ni-NTA agarose. Sepharose 84-91 transforming growth factor beta receptor 2 Homo sapiens 24-32 20451568-6 2010 After cleavage of purified thioredoxin/TbetaRII-ED fusion by recombinant human enteropeptidase light chain (L-HEP) the target protein of TbetaRII-ED was separated from thioredoxin on Ni-NTA agarose. Sepharose 190-197 thioredoxin Homo sapiens 27-38 20451568-6 2010 After cleavage of purified thioredoxin/TbetaRII-ED fusion by recombinant human enteropeptidase light chain (L-HEP) the target protein of TbetaRII-ED was separated from thioredoxin on Ni-NTA agarose. Sepharose 190-197 transforming growth factor beta receptor 2 Homo sapiens 137-145 20446669-3 2010 The electrode substrate patched with an agarose stamp having 50-microm-high bumps was used for the spatially confined detachment of heparin/polyethyleneimine precoated on glass substrates, followed by micropatterned adsorption of fibronectin. Sepharose 40-47 fibronectin 1 Homo sapiens 230-241 20479083-5 2010 The recombinant SSL5-Sepharose also bound to proMMP-9 secreted by interleukin 8 (IL-8)-stimulated neutrophils and HT1080 fibrosarcoma cells. Sepharose 21-30 C-X-C motif chemokine ligand 8 Homo sapiens 81-85 20479083-5 2010 The recombinant SSL5-Sepharose also bound to proMMP-9 secreted by interleukin 8 (IL-8)-stimulated neutrophils and HT1080 fibrosarcoma cells. Sepharose 21-30 C-X-C motif chemokine ligand 8 Homo sapiens 66-79 20155435-8 2010 The tPA encapsulated in microbubbles kept fibrinolysis activity under the conditions of both natural releasing and ultrasound exposure, checked by agarose fibrin plate process. Sepharose 147-154 chromosome 20 open reading frame 181 Homo sapiens 4-7 20580823-4 2010 Distribution of collagen type I and fibronectin in the ECM of the agarose cultures was determined by immunoflorescence. Sepharose 66-73 fibronectin 1 Homo sapiens 36-47 20580823-4 2010 Distribution of collagen type I and fibronectin in the ECM of the agarose cultures was determined by immunoflorescence. Sepharose 66-73 multimerin 1 Homo sapiens 55-58 20580823-6 2010 The agarose overlay significantly enhanced the cell number in the IGF-I, TGF-beta and PDGF treated cultures by 2-3 fold. Sepharose 4-11 insulin like growth factor 1 Homo sapiens 66-71 20580823-6 2010 The agarose overlay significantly enhanced the cell number in the IGF-I, TGF-beta and PDGF treated cultures by 2-3 fold. Sepharose 4-11 transforming growth factor beta 1 Homo sapiens 73-81 20580823-7 2010 The overlay also significantly enhanced the processing of procollagen to collagen fibrils from 29% in standard cultures to 63-68% in agarose cultures for the IGF-I and PDGF cultures, and from 66% in standard culture to 85% in agarose culture for the TGF-beta cultures. Sepharose 133-140 insulin like growth factor 1 Homo sapiens 158-163 20806494-12 2010 In vivo, immunization of Sepharose 4B coupling rSj22.6/26GST increased the number of CD4+CD25+ T cells. Sepharose 25-37 CD4 antigen Mus musculus 85-88 19750485-3 2010 Indels (insertion/deletion) in intron 7 of Y-linked allele provide a significant discrimination between ZFX and ZFY, thus the amplification products can be simply distinguished by agarose gel electrophoresis, exhibiting sex-specific banding patterns (female, 354 bp; male, 354 bp, 135 bp). Sepharose 180-187 zinc finger X-chromosomal protein Ailuropoda melanoleuca 104-107 20806494-12 2010 In vivo, immunization of Sepharose 4B coupling rSj22.6/26GST increased the number of CD4+CD25+ T cells. Sepharose 25-37 interleukin 2 receptor, alpha chain Mus musculus 89-93 20806494-13 2010 CD4+CD25+ T cells separated from Sepharose 4B coupling rSj22.6/26GST immunized mice had stronger inhibitory ability (cpm 1 420 +/- 335), compared with that of mice immunized with soluble antigen (cpm 3 558 +/- 147). Sepharose 33-42 CD4 antigen Mus musculus 0-3 20806494-13 2010 CD4+CD25+ T cells separated from Sepharose 4B coupling rSj22.6/26GST immunized mice had stronger inhibitory ability (cpm 1 420 +/- 335), compared with that of mice immunized with soluble antigen (cpm 3 558 +/- 147). Sepharose 33-42 interleukin 2 receptor, alpha chain Mus musculus 4-8 20806494-14 2010 CONCLUSION: In contrast to the Freund"s adjuvant emulsified antigen, immunization with Sepharose 4B coupling rSj22.6/26GST increases the number of CD4+CD25+ T cells, which showed stronger inhibition on the CD4+ CD25- T cell proliferation, and the mechanism of which may be involved in DCs maturation. Sepharose 87-99 CD4 antigen Mus musculus 147-150 20806494-14 2010 CONCLUSION: In contrast to the Freund"s adjuvant emulsified antigen, immunization with Sepharose 4B coupling rSj22.6/26GST increases the number of CD4+CD25+ T cells, which showed stronger inhibition on the CD4+ CD25- T cell proliferation, and the mechanism of which may be involved in DCs maturation. Sepharose 87-99 interleukin 2 receptor, alpha chain Mus musculus 151-155 20806494-14 2010 CONCLUSION: In contrast to the Freund"s adjuvant emulsified antigen, immunization with Sepharose 4B coupling rSj22.6/26GST increases the number of CD4+CD25+ T cells, which showed stronger inhibition on the CD4+ CD25- T cell proliferation, and the mechanism of which may be involved in DCs maturation. Sepharose 87-99 CD4 antigen Mus musculus 206-209 20806494-14 2010 CONCLUSION: In contrast to the Freund"s adjuvant emulsified antigen, immunization with Sepharose 4B coupling rSj22.6/26GST increases the number of CD4+CD25+ T cells, which showed stronger inhibition on the CD4+ CD25- T cell proliferation, and the mechanism of which may be involved in DCs maturation. Sepharose 87-99 interleukin 2 receptor, alpha chain Mus musculus 211-215 19150150-8 2010 Titin content was determined by SDS-agarose-gel electrophoresis. Sepharose 36-43 titin Rattus norvegicus 0-5 20450885-7 2010 Finally, TLR4 molecules were co-precipitated with KML-C, to which agarose beads were conjugated, indicating that those molecules are associated. Sepharose 66-73 toll-like receptor 4 Mus musculus 9-13 20566462-5 2010 The FGF-21 protein expression was induced by IPTG and purified by Ni-NTA agarose. Sepharose 73-80 fibroblast growth factor 21 Homo sapiens 4-10 20073988-5 2010 In this case the MMP-13 C-terminal domain (CTD) comprises hinge and hemopexin domain, and we immobilized the fusion construct on a column of agarose bound immunoglobin G. The MMP-13 CTD affinity column so generated enabled the efficient and gentle isolation of interacting proteins from the culture medium of human articular chondrocytes. Sepharose 141-148 matrix metallopeptidase 13 Homo sapiens 17-23 20073988-5 2010 In this case the MMP-13 C-terminal domain (CTD) comprises hinge and hemopexin domain, and we immobilized the fusion construct on a column of agarose bound immunoglobin G. The MMP-13 CTD affinity column so generated enabled the efficient and gentle isolation of interacting proteins from the culture medium of human articular chondrocytes. Sepharose 141-148 matrix metallopeptidase 13 Homo sapiens 175-181 20298689-4 2010 Corneal NV model in rats was induced by implantation of agarose-coated gelfoam pellets containing basic fibroblast growth factor (bFGF) into corneal stroma. Sepharose 56-63 fibroblast growth factor 2 Rattus norvegicus 98-128 20298689-4 2010 Corneal NV model in rats was induced by implantation of agarose-coated gelfoam pellets containing basic fibroblast growth factor (bFGF) into corneal stroma. Sepharose 56-63 fibroblast growth factor 2 Rattus norvegicus 130-134 19711194-8 2010 Agarose gel electrophoresis indicated that the complete ORF of Attacin A gene has been cloned successfully from Drosophila stimulated by E. coli which includes 666 bp and encodes 221 AA. Sepharose 0-7 Attacin-A Drosophila melanogaster 63-72 20308691-5 2010 MCF-7 cell clones expressing human ERalpha fused to a tandem affinity purification tag were generated and used to purify native nuclear ER-containing complexes by IgG-Sepharose affinity chromatography and glycerol gradient centrifugation. Sepharose 167-176 estrogen receptor 1 Homo sapiens 35-42 20815266-3 2010 In order to enhance the solubility of GST-Ulp1p-His6, we purified the fusion protein GST-Ulp1p-His6 by either glutathione S-transferase agarose or Ni-NTA resin chromatography, the purity was up to 98%. Sepharose 136-143 SUMO protease ULP1 Saccharomyces cerevisiae S288C 42-47 20815266-3 2010 In order to enhance the solubility of GST-Ulp1p-His6, we purified the fusion protein GST-Ulp1p-His6 by either glutathione S-transferase agarose or Ni-NTA resin chromatography, the purity was up to 98%. Sepharose 136-143 SUMO protease ULP1 Saccharomyces cerevisiae S288C 89-94 20206212-6 2010 The interaction was also confirmed by immunoprecipitation analysis of actinomycin D-treated QPRT-FLAG expressing cells using anti-FLAG-agarose. Sepharose 135-142 quinolinate phosphoribosyltransferase Homo sapiens 92-96 20227383-2 2010 We screened biologically active sequences in the mouse laminin alpha2 chain G domain using 110 soluble peptides by the peptide-coated plate and the peptide-conjugated Sepharose bead assays. Sepharose 167-176 laminin, alpha 2 Mus musculus 55-69 20503257-3 2010 METHODS: We investigated the binding of MBL to fructoselysine by chromatography of human serum on fructoselysine-Sepharose, followed by Western blot and mass spectrometry analysis. Sepharose 113-122 mannose-binding lectin family member 3, pseudogene Homo sapiens 40-43 20348404-6 2010 Hemopexin was purified from study participants" serum by use of heme agarose beads. Sepharose 69-76 hemopexin Homo sapiens 0-9 19995544-5 2010 A highly purified alpha1-antitrypsin was isolated at 95% step recovery by adsorbing the flow-through on 4% epoxy-crosslinked agarose-10% tungsten carbide adsorbent material coupled with a cationic ligand. Sepharose 125-132 serpin family A member 1 Homo sapiens 18-36 20012488-6 2010 The apoptotic death in HEK-hSloalpha cells was detected using immunocytochemistry, analysis of fragmented DNA by agarose gel electrophoresis, MTT test, and flow cytometry assays. Sepharose 113-120 potassium calcium-activated channel subfamily M alpha 1 Homo sapiens 27-36 20002787-6 2010 We now report that purified L-FCN-MASPs complexes, bound from serum to N-acetylcysteine-Sepharose, or MBL-MASPs complexes, bound to mannan-agarose, generate clots when incubated with calcified plasma or purified fibrinogen and factor XIII. Sepharose 139-146 mannose binding lectin 2 Homo sapiens 102-105 20083259-3 2010 GSTM1 and GSTT1 genes were polymerase chain reaction amplified and gene fragments were separated by agarose gel electrophoresis. Sepharose 100-107 glutathione S-transferase mu 1 Homo sapiens 0-5 19343538-3 2010 To obtain human UBE1 protein, the full length of human UBE1 was expressed in E. coli and purified by Ni-NTA superflow sepharose and strep-tactin sepharose which based on UB-UBE1 high-energy thioester bonded intermediate complex. Sepharose 118-127 ubiquitin like modifier activating enzyme 1 Homo sapiens 55-59 19343538-3 2010 To obtain human UBE1 protein, the full length of human UBE1 was expressed in E. coli and purified by Ni-NTA superflow sepharose and strep-tactin sepharose which based on UB-UBE1 high-energy thioester bonded intermediate complex. Sepharose 118-127 ubiquitin like modifier activating enzyme 1 Homo sapiens 55-59 19343538-3 2010 To obtain human UBE1 protein, the full length of human UBE1 was expressed in E. coli and purified by Ni-NTA superflow sepharose and strep-tactin sepharose which based on UB-UBE1 high-energy thioester bonded intermediate complex. Sepharose 145-154 ubiquitin like modifier activating enzyme 1 Homo sapiens 55-59 19343538-3 2010 To obtain human UBE1 protein, the full length of human UBE1 was expressed in E. coli and purified by Ni-NTA superflow sepharose and strep-tactin sepharose which based on UB-UBE1 high-energy thioester bonded intermediate complex. Sepharose 145-154 ubiquitin like modifier activating enzyme 1 Homo sapiens 55-59 20925204-5 2010 Genotyping of the CD46 gene was done using 2.5% agarose gel. Sepharose 48-55 CD46 molecule Homo sapiens 18-22 19853652-7 2010 Further, autoschizis is effected through increases in DNase I and DNase II activity with a concomitant decrease in caspase-3 activity which leads to a random cleavage of the DNA as demonstrated by a smear like pattern after electrophoresis on agarose gel. Sepharose 243-250 caspase 3 Homo sapiens 115-124 20230688-2 2010 METHODS: The human UNC5CL nucleotide sequence encoding amino acid from 280 to 518 was amplified and cloned into pGEX-4T-2 vector, then transformed into E.coli BL21, in which the fusion protein GST-UNC5CL (aa280-518) was induced and purified by Glutathione Sepharose-4B. Sepharose 256-265 unc-5 family C-terminal like Homo sapiens 19-25 20575033-5 2010 The analysis of VWF multimers generally consists of four steps: (1) electrophoresis of plasma in an agarose gel, (2) either gel fixation or transfer of the electrophoretic protein product to a membrane, (3) immunodetection of the protein, and (4) evaluation of the protein in the gel or membrane. Sepharose 100-107 von Willebrand factor Homo sapiens 16-19 19909400-2 2010 Preparations of FN purified from human plasma by gelatin-Sepharose affinity chromatography typically also contain gelatin-binding gelatinases that may cleave FN, reduce its stability and alter its biological activities. Sepharose 57-66 fibronectin 1 Homo sapiens 16-18 19909400-5 2010 MATERIAL AND METHODS: Experiments tested the elution profiles for FN and gelatinases from gelatin-Sepharose using a concentration range (1-7%) of dimethyl sulfoxide (DMSO) and 4 m urea as eluants. Sepharose 98-107 fibronectin 1 Homo sapiens 66-68 20346879-2 2010 METHODS: For this purpose, erythrocyte G6PD was initially purified 835-fold at a yield of 41.7% using 2",5"-Adenosine diphosphate sepharose 4B affinity gel chromatography. Sepharose 130-142 glucose-6-phosphate dehydrogenase Homo sapiens 39-43 19913620-7 2010 Trx-scFv was purified using a bovine ribonuclease A-coupled Sepharose column, and 2.7 mg/L of purified protein was obtained. Sepharose 60-69 thioredoxin Bos taurus 0-3 19836035-3 2010 HSP70 purification was carried out by immunoaffinity chromatography using adenosine di-phosphate (ADP-agarose column) and the characterization of the purified protein was done using western blotting by mouse monoclonal anti-HSP70. Sepharose 102-109 heat shock 70 kDa protein 1B Bos taurus 0-5 20064650-3 2010 Affinity to GAG has been evaluated by sorption on heparin-Sepharose. Sepharose 58-67 melanoma antigen Mus musculus 12-15 20165787-1 2010 UV-B photoirradiation of a neurotransmitter (serotonin) and aromatic amino acids (tryptophan and tyrosine) with oxygen results in DNA cleavage by generation of reactive oxygen species (ROS) as demonstrated by agarose gel electrophoresis with pBR 322 DNA, ESR and laser flash photolysis measurements. Sepharose 209-216 translocator protein Homo sapiens 242-245 20518354-5 2010 We obtained PHF8 polyclonal antibody by affinity purifying the antiserum with CNBr-activated Sepharose 4B beads. Sepharose 93-105 PHD finger protein 8 Homo sapiens 12-16 20635694-2 2010 METHODS: ApoB genotypes were determined by PCR technology and agarose gel electrophoresis. Sepharose 62-69 apolipoprotein B Homo sapiens 9-13 20002787-8 2010 Fibrinopeptides A and B (FPA and FPB, respectively) were released after fibrinogen cleavage by L-FCN-MASPs complexes captured on N-acetylcysteine-Sepharose. Sepharose 146-155 fibrinogen beta chain Homo sapiens 72-82 20093243-0 2010 Analysis of von Willebrand factor multimers by simultaneous high- and low-resolution vertical SDS-agarose gel electrophoresis and Cy5-labeled antibody high-sensitivity fluorescence detection. Sepharose 98-105 von Willebrand factor Homo sapiens 12-33 20093243-2 2010 A novel method for multimer analysis of vWF by 2-chamber, vertical (sodium dodecyl sulfate), agarose gel electrophoresis, designed for comparing discontinuous high- and low-resolving gels for plasma and platelets, followed by Western blotting and high-sensitivity fluorescence detection (HSFD) of cyanine (Cy)5-labeled vWF multimers is presented. Sepharose 93-100 von Willebrand factor Homo sapiens 40-43 20432946-6 2010 Kal protein was purified from the supernatant with Phenyl Superose and Heparin Sepharose FF chromatograph. Sepharose 79-88 serpin family A member 4 Homo sapiens 0-3 19705452-8 2010 Here we establish that G20 inhibits protein aggregation as judged by native gel electrophoresis, an agarose gel electrophoresis for resolving aggregates (AGERA) assay, and an immunoblotting assay. Sepharose 100-107 chromosome 3 open reading frame 18 Homo sapiens 23-26 19338577-5 2010 In vitro, recombinant exon 1 of huntingtin with 44 glutamines (htt-exon1-44Q) binds to CaM-agarose; the addition of 10 microM of CaM-peptide significantly decreases the interaction of htt-exon1-44Q and CaM but not the binding between CaM and calcineurin, another CaM-binding protein. Sepharose 91-98 huntingtin Homo sapiens 32-42 20200599-4 2010 However, immobilizing neurotrophic factors secreting cells within a scaffold is relatively cumbersome, and alternative strategies are needed to provide sustained release of BDNF from templated agarose scaffolds. Sepharose 193-200 brain derived neurotrophic factor Homo sapiens 173-177 20200599-7 2010 Lysozyme, a protein similar in size and isoelectric point to BDNF, is released from the multilayers on the agarose and is biologically active during the earlier time points, with decreasing activity at later time points. Sepharose 107-114 brain derived neurotrophic factor Homo sapiens 61-65 20111710-0 2010 Two forms of activation-induced cytidine deaminase differing in their ability to bind agarose. Sepharose 86-93 activation-induced cytidine deaminase Mus musculus 13-50 20111710-3 2010 METHODOLOGY/PRINCIPAL FINDINGS: We found that, regardless of whether cell lysates containing exogenous or endogenous AID were examined, one of two mouse AID forms bound to agarose alone. Sepharose 172-179 activation-induced cytidine deaminase Mus musculus 153-156 20349026-3 2010 The isolated "strong VN binders" were covalently coupled to CNBr-activated Sepharose. Sepharose 75-84 vitronectin Homo sapiens 21-23 20078429-3 2010 We demonstrate for the first time that this combination of three detergents significantly improves binding efficiency of GST and GST fusion proteins to gluthathione (GSH) Sepharose. Sepharose 171-180 glutathione S-transferase kappa 1 Homo sapiens 121-124 20078429-3 2010 We demonstrate for the first time that this combination of three detergents significantly improves binding efficiency of GST and GST fusion proteins to gluthathione (GSH) Sepharose. Sepharose 171-180 glutathione S-transferase kappa 1 Homo sapiens 129-132 19338577-5 2010 In vitro, recombinant exon 1 of huntingtin with 44 glutamines (htt-exon1-44Q) binds to CaM-agarose; the addition of 10 microM of CaM-peptide significantly decreases the interaction of htt-exon1-44Q and CaM but not the binding between CaM and calcineurin, another CaM-binding protein. Sepharose 91-98 calmodulin 1 Homo sapiens 87-90 19338577-5 2010 In vitro, recombinant exon 1 of huntingtin with 44 glutamines (htt-exon1-44Q) binds to CaM-agarose; the addition of 10 microM of CaM-peptide significantly decreases the interaction of htt-exon1-44Q and CaM but not the binding between CaM and calcineurin, another CaM-binding protein. Sepharose 91-98 calmodulin 1 Homo sapiens 129-132 19338577-5 2010 In vitro, recombinant exon 1 of huntingtin with 44 glutamines (htt-exon1-44Q) binds to CaM-agarose; the addition of 10 microM of CaM-peptide significantly decreases the interaction of htt-exon1-44Q and CaM but not the binding between CaM and calcineurin, another CaM-binding protein. Sepharose 91-98 calmodulin 1 Homo sapiens 129-132 19338577-5 2010 In vitro, recombinant exon 1 of huntingtin with 44 glutamines (htt-exon1-44Q) binds to CaM-agarose; the addition of 10 microM of CaM-peptide significantly decreases the interaction of htt-exon1-44Q and CaM but not the binding between CaM and calcineurin, another CaM-binding protein. Sepharose 91-98 calmodulin 1 Homo sapiens 129-132 19338577-5 2010 In vitro, recombinant exon 1 of huntingtin with 44 glutamines (htt-exon1-44Q) binds to CaM-agarose; the addition of 10 microM of CaM-peptide significantly decreases the interaction of htt-exon1-44Q and CaM but not the binding between CaM and calcineurin, another CaM-binding protein. Sepharose 91-98 calmodulin 1 Homo sapiens 129-132 22615596-4 2010 After intradermal injection of confirmed purified band of protein to rabbits and isolation of the serum IgG antibody, for its affinity purification, the rabbit"s purified Hsp90 specific IgG was coupled to the cyanogen bromide-activated Sepharose 4B. Sepharose 236-245 heat shock protein 90 alpha family class A member 1 Homo sapiens 171-176 19918048-5 2010 IL-33 levels were measured using a sandwich ELISA after removal of rheumatoid factor with protein A-Sepharose beads. Sepharose 100-109 interleukin 33 Homo sapiens 0-5 19669392-0 2010 Dynamic compression alters NFkappaB activation and IkappaB-alpha expression in IL-1beta-stimulated chondrocyte/agarose constructs. Sepharose 111-118 NFKB inhibitor alpha Homo sapiens 51-64 19669392-0 2010 Dynamic compression alters NFkappaB activation and IkappaB-alpha expression in IL-1beta-stimulated chondrocyte/agarose constructs. Sepharose 111-118 interleukin 1 beta Homo sapiens 79-87 19669392-1 2010 OBJECTIVE AND DESIGN: Determine the effect of IL-1beta and dynamic compression on NFkappaB activation and IkappaB-alpha gene expression in chondrocyte/agarose constructs. Sepharose 151-158 interleukin 1 beta Homo sapiens 46-54 19669392-1 2010 OBJECTIVE AND DESIGN: Determine the effect of IL-1beta and dynamic compression on NFkappaB activation and IkappaB-alpha gene expression in chondrocyte/agarose constructs. Sepharose 151-158 NFKB inhibitor alpha Homo sapiens 106-119 20093697-3 2010 Qualitative assessment of proteinuria by sodium dodecyl sulfate-agarose gel electrophoresis revealed a broad band with a molecular weight of approximately 15 kDa that was compatible with lysozyme (LZM). Sepharose 64-71 lysozyme Canis lupus familiaris 187-195 20093697-3 2010 Qualitative assessment of proteinuria by sodium dodecyl sulfate-agarose gel electrophoresis revealed a broad band with a molecular weight of approximately 15 kDa that was compatible with lysozyme (LZM). Sepharose 64-71 lysozyme Canis lupus familiaris 197-200 20204849-2 2010 We found that Arabidopsis HY5 protein functions as a novel DNA-binding tag (DBtag), and DBtagged proteins are immobilized and purified on a newly designed agarose/DNA microplate. Sepharose 155-162 Basic-leucine zipper (bZIP) transcription factor family protein Arabidopsis thaliana 26-29 20028562-8 2009 In contrast, agarose-induced spheroids of MCF-7, a weakly invasive E-cadherin positive breast carcinoma cell line, do not exhibit ultrastructural polar features. Sepharose 13-20 cadherin 1 Homo sapiens 67-77 20033654-4 2010 We describe here a simple and reliable multiplex PCR-SSP (sequence-specific priming) method for relatively rapid and inexpensive genotyping of 15 KIR genes using standard agarose gel electrophoresis. Sepharose 171-178 killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4 Homo sapiens 146-149 19709391-3 2010 The PF4-containing antigen complexes were attached to microtiter plates via a spacer, rather than using nitrocellulose, and the final reaction enzyme substrate was added in melted agarose which, after rapid hardening, localized color development of enzyme-tagged anti-immunoglobulin G (IgG) probes to single PF4/heparin-specific B cells. Sepharose 180-187 platelet factor 4 Homo sapiens 4-7 19801409-5 2009 We show that CbpA binds to cAMP-conjugated agarose via its C-terminal CAP domain. Sepharose 43-50 cAMP-binding protein A Pseudomonas aeruginosa PAO1 13-17 19858221-4 2009 A galactose-binding protein was isolated from the plasma membranes of cells at 100% density by affinity chromatography using an asialo-transferrin-Sepharose column and found to be galectin-3 as revealed by mass spectrometric analysis. Sepharose 147-156 lectin, galactose binding, soluble 3 Mus musculus 180-190 19961422-7 2009 Therefore, admixtures of MMPs can be efficiently eliminated from CP preparations by chromatography on heparin-Sepharose as proposed previously. Sepharose 110-119 matrix metallopeptidase 2 Homo sapiens 25-29 19961422-7 2009 Therefore, admixtures of MMPs can be efficiently eliminated from CP preparations by chromatography on heparin-Sepharose as proposed previously. Sepharose 110-119 ceruloplasmin Homo sapiens 65-67 19952503-5 2009 The Sry sequence in the extracted DNA was amplified by PCR and the product was analyzed by agarose gel electrophoresis. Sepharose 91-98 sex determining region Y Rattus norvegicus 4-7 19853918-2 2009 Pull-down assays using an N-acetyl-D-glucosamine (GlcNAc)-agarose column demonstrated that CRP binds to the trimeric FBG domains, and that the GlcNAc-binding ability of the FBG domain is unaffected by CRP binding. Sepharose 58-65 C-reactive protein Homo sapiens 91-94 19671121-7 2009 Albumin was measured by using semiquantitative test strips, by agarose gel electrophoresis, and by an automated immunoturbidimetric assay designed for human samples (considered as the gold standard). Sepharose 63-70 albumin Homo sapiens 0-7 20232632-2 2009 METHODS: The fusion protein GST-p55/570 was expressed from the prokaryotic expression plasmid pGEX-570, and purified by using glutathione-agarose. Sepharose 138-145 membrane protein, palmitoylated Mus musculus 32-35 19839573-0 2009 Recyclable molecular trapping and SERS detection in silver-loaded agarose gels with dynamic hot spots. Sepharose 66-73 seryl-tRNA synthetase 2, mitochondrial Homo sapiens 34-38 19807150-2 2009 We screened for biologically active sites on the mouse laminin alpha3 chain G domain using 107 synthetic peptides on coated plates and conjugated to Sepharose beads with HT1080 human fibrosarcoma cells, HaCaT human skin keratinocyte cells, and human dermal fibroblasts (HDFs). Sepharose 149-158 laminin, alpha 3 Mus musculus 55-69 19902129-2 2009 The Kunitz domain 3 and Cterminal of hTFPI-2 (hTFPI-2/KD3C), which has the activity toward heparin calcium, have been successfully expressed in Pichia pastoris and purified by SPSepharose and heparin-Sepharose chromatography. Sepharose 178-187 tissue factor pathway inhibitor 2 Homo sapiens 37-44 19902129-2 2009 The Kunitz domain 3 and Cterminal of hTFPI-2 (hTFPI-2/KD3C), which has the activity toward heparin calcium, have been successfully expressed in Pichia pastoris and purified by SPSepharose and heparin-Sepharose chromatography. Sepharose 178-187 tissue factor pathway inhibitor 2 Homo sapiens 46-58 19776176-9 2009 A pull-down assay using delphinidin-Sepharose beads revealed that delphinidin binds directly with MAPKK4 or PI-3K in a manner that was competitive with adenosine triphosphate. Sepharose 36-45 mitogen-activated protein kinase kinase 4 Mus musculus 98-104 19728025-7 2009 Additionally, DNA laddering, which is a hallmark of apoptosis, was observed by agarose gel electrophoresis of DNA harvested from SP2/0 cells treated with ART. Sepharose 79-86 Sp2 transcription factor Mus musculus 129-132 19396626-4 2009 These methods are coimmunoprecipitation, pull down with biotinylated geldanamycin, and immobilization of Hsp90beta on sepharose. Sepharose 118-127 heat shock protein 90 alpha family class B member 1 Homo sapiens 105-114 19595785-4 2009 cDNAs corresponding to the two AFABP isoforms were cloned and expressed in Escherichia coli as GST fusions, purified by using glutathione sepharose 4B chromatography and evaluated for lipid binding using the fluorescent surrogate ligand 1-anilinonaphthalene 8-sulphonic acid (1,8-ANS). Sepharose 138-150 fatty acid binding protein 4 Gallus gallus 31-36 19796172-3 2009 In this study, we purified a 62 kDa MNSFbeta adduct from murine liver lysates by sequential chromatography on DEAE and anti-MNSFbeta IgG-conjugated Sepharose. Sepharose 148-157 Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived) Mus musculus 36-44 19796172-3 2009 In this study, we purified a 62 kDa MNSFbeta adduct from murine liver lysates by sequential chromatography on DEAE and anti-MNSFbeta IgG-conjugated Sepharose. Sepharose 148-157 Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (fox derived) Mus musculus 124-132 19625311-11 2009 Glycodelin-C bound to hyaluronic acid-coated agarose beads in the presence of GIP. Sepharose 45-52 progestagen associated endometrial protein Homo sapiens 0-10 19295489-0 2009 Elevated glucose and fatty acid levels impair substance P-induced dermal microvascular endothelial cell migration and proliferation in an agarose gel model system. Sepharose 138-145 tachykinin precursor 1 Homo sapiens 46-57 19295489-14 2009 Our data suggest that glucose and fatty acids perturb SP-induced HMEC-1 migration and proliferation in an agarose gel migration model. Sepharose 106-113 tachykinin precursor 1 Homo sapiens 54-56 19911129-11 2009 Secreted ALDH2 was easily purified from the culture supernatant by using Ni(2+)-Sepharose affinity chromatography. Sepharose 80-89 aldehyde dehydrogenase 2 family member Homo sapiens 9-14 19726683-9 2009 We created a rat monoclonal antibody against the carboxyl terminus of mouse GPIHBP1 and used that antibody to coat agarose beads. Sepharose 115-122 GPI-anchored HDL-binding protein 1 Mus musculus 76-83 19665992-2 2009 In this work we elicited an exclusive cell-cell contact by culturing A7r5 VSMCs on agarose-coated wells to form floating cell clusters, and we demonstrated that a steady state with a reduced response to the vasoactive peptide Angiotensin II (ATII) was induced. Sepharose 83-90 angiotensinogen Homo sapiens 226-240 19665992-2 2009 In this work we elicited an exclusive cell-cell contact by culturing A7r5 VSMCs on agarose-coated wells to form floating cell clusters, and we demonstrated that a steady state with a reduced response to the vasoactive peptide Angiotensin II (ATII) was induced. Sepharose 83-90 angiotensinogen Homo sapiens 242-246 19754914-3 2009 In this work, purification of the porcine CYP1A and CYP2A19 to electrophoretic homogeneity from the pig hepatic microsomes using octylamino Sepharose and hydroxylapatite column chromatography is reported. Sepharose 140-149 cytochrome P450 family 2 subfamily A member 19 Sus scrofa 52-59 19415461-5 2009 In the current study, we performed purification of CMP-N-acetylneuraminic acid:lactosylceramide alpha2,3-sialyltransferase (GM3 synthase) from Triton X-100 extract of human blood mononuclear cells by immunoaffinity chromatography on Sepharose coupled with anti-GM3 synthase antibody. Sepharose 233-242 ST3 beta-galactoside alpha-2,3-sialyltransferase 5 Homo sapiens 79-122 19415461-5 2009 In the current study, we performed purification of CMP-N-acetylneuraminic acid:lactosylceramide alpha2,3-sialyltransferase (GM3 synthase) from Triton X-100 extract of human blood mononuclear cells by immunoaffinity chromatography on Sepharose coupled with anti-GM3 synthase antibody. Sepharose 233-242 ST3 beta-galactoside alpha-2,3-sialyltransferase 5 Homo sapiens 124-136 19231914-1 2009 The objectives of this study were to determine how culture time and dynamic compression, applied to murine chondrocyte-agarose constructs, influence construct stiffness, expression of col2 and type II collagen. Sepharose 119-126 collagen, type II, alpha 1 Mus musculus 184-188 19656515-13 2009 The results confirmed the role of RGD-dependent integrins as mechanotransducers in the regulation of both ECM gene expression and matrix biosynthesis for chondrocytes seeded in agarose under the applied loading regime. Sepharose 177-184 multimerin 1 Homo sapiens 106-109 20112680-3 2009 The recombinant protein (Nsp2-N and Nsp2-C) were over expressed in E. coli BL21 and purified by Ni-NTA agarose affinity chromatogram and gel filtration. Sepharose 103-110 reticulon 2 Homo sapiens 25-29 19755493-4 2009 alpha3beta1 integrin was identified as the major Gal-3-binding protein in corneal epithelial cells by affinity chromatography of cell lysates on a Gal-3-Sepharose column. Sepharose 153-162 galectin 3 Homo sapiens 49-54 19595454-3 2009 Human marrow stromal cells (hMSCs) were singularly encapsulated in agarose capsules containing the immobilized matrix molecules, fibronectin and fibrinogen to ameliorate cell-matrix survival signals. Sepharose 67-74 fibrinogen beta chain Homo sapiens 145-155 19576873-3 2009 METHODS: Rabbit ASGPR was purified by affinity chromatography on galactose-Sepharose and used for standardised detection of anti-ASGPR by ELISA. Sepharose 75-84 asialoglycoprotein receptor 1 Homo sapiens 16-21 19664588-4 2009 However, BAM4 binds to amylopectin and to amylose-Sepharose whereas BAM2 has very low beta-amylase activity and poor glucan binding. Sepharose 50-59 beta-amylase 4 Arabidopsis thaliana 9-13 20396696-5 2009 Affinity-purified on Ni-NTA agarose recombinant Cx43-CBD was used for immunization of mice and obtaining of monoclonal antibodies. Sepharose 28-35 gap junction protein, alpha 3 Mus musculus 48-52 19648270-3 2009 Using a BA-affinity Sepharose matrix, a 26-kDa protein was selectively precipitated from human neutrophils and identified as the lysosomal protease cathepsin G (catG) by mass spectrometry (MALDI-TOF) and by immunological analysis. Sepharose 20-29 cathepsin G Homo sapiens 148-159 19648270-3 2009 Using a BA-affinity Sepharose matrix, a 26-kDa protein was selectively precipitated from human neutrophils and identified as the lysosomal protease cathepsin G (catG) by mass spectrometry (MALDI-TOF) and by immunological analysis. Sepharose 20-29 cathepsin G Homo sapiens 161-165 19697318-0 2009 Confocal microscopy study of uptake kinetics of alpha-lactalbumin and beta-lactoglobulin onto the cation-exchanger SP Sepharose FF. Sepharose 118-127 lactalbumin alpha Homo sapiens 48-65 19751561-2 2009 METHODS: HepG2 cells were cultured, and purified HMGB1 protein was prepared by chromatography on Ni(2+)-NTA Sepharose column under natural conditions with recombinant expression plasmid pET14b-HMGB1. Sepharose 108-117 high mobility group box 1 Homo sapiens 49-54 19706048-7 2009 IL-1RA Variable Number of Tandem Repeats (VNTR) polymorphism in intron 2 and TNFalpha-308G-A polymorphism were genotyped by using polymerase chain reaction and agarose gel electrophoresis. Sepharose 160-167 interleukin 1 receptor antagonist Homo sapiens 0-6 19451294-7 2009 Similarly, by using an airway infection model in which bacteria were impacted in the airways by agarose beads, the administration of SCC1 was significantly superior to water in decreasing the lung bacterial burden and the levels of bacteremia and markers of airway inflammation. Sepharose 96-103 RAD21 cohesin complex component Mus musculus 133-137 18677626-0 2009 Dynamic compression inhibits fibronectin fragment induced iNOS and COX-2 expression in chondrocyte/agarose constructs. Sepharose 99-106 fibronectin 1 Homo sapiens 29-40 18677626-0 2009 Dynamic compression inhibits fibronectin fragment induced iNOS and COX-2 expression in chondrocyte/agarose constructs. Sepharose 99-106 nitric oxide synthase 2 Homo sapiens 58-62 18677626-0 2009 Dynamic compression inhibits fibronectin fragment induced iNOS and COX-2 expression in chondrocyte/agarose constructs. Sepharose 99-106 mitochondrially encoded cytochrome c oxidase II Homo sapiens 67-72 19457929-6 2009 The ability of wild-type (WT) or mutant PTEN to bind ATP was assessed by ATP-agarose-binding assays. Sepharose 77-84 phosphatase and tensin homolog Homo sapiens 40-44 19492866-5 2009 We demonstrate that the central region of human BRCA1 binds strongly to negatively sc plasmid DNA at a native superhelix density, as evidenced by electrophoretic retardation of sc DNA in agarose gels. Sepharose 187-194 BRCA1 DNA repair associated Homo sapiens 48-53 19615639-2 2009 Agarose gel is a new and absorbable filler indicated for the correction of soft tissues and lip. Sepharose 0-7 SMG1 nonsense mediated mRNA decay associated PI3K related kinase Homo sapiens 92-95 19620657-6 2009 Reverse transcription PCR revealed that the PDE4D transcript was amplified, and the expected size fragment was obtained in a 1% agarose gel. Sepharose 128-135 phosphodiesterase 4D Bos taurus 44-49 19395472-5 2009 Mouse Hx purified on hemin-agarose beads and rhHx decreased the production of cytokines from BMDMs and peritoneal macrophages induced by LPS. Sepharose 27-34 hemopexin Mus musculus 6-8 19615639-8 2009 A volume of 0.5-1.0 mL of agarose gel was sufficient for each lip. Sepharose 26-33 SMG1 nonsense mediated mRNA decay associated PI3K related kinase Homo sapiens 62-65 21155242-7 2009 The results of DNA agarose gel and flow cytometry showed that 10 micromol/L Ang II induced the apoptosis of HUVEC, and the apoptosis rate was significantlyhigher than normal control group (P < 0.05). Sepharose 19-26 angiogenin Homo sapiens 76-79 19540766-2 2009 Agarose gel and precipitation assays indicate inhibition of RNase A activity by these molecules with a possible role of the polar side chains of the amino acids in RNase A inhibition. Sepharose 0-7 ribonuclease A family member 1, pancreatic Homo sapiens 60-67 19457491-4 2009 Titin isoform sizes were quantified via sodium dodecyl sulfate-vertical agarose gel electrophoresis (SDS-VAGE) analysis. Sepharose 72-79 titin Homo sapiens 0-5 19540766-2 2009 Agarose gel and precipitation assays indicate inhibition of RNase A activity by these molecules with a possible role of the polar side chains of the amino acids in RNase A inhibition. Sepharose 0-7 ribonuclease A family member 1, pancreatic Homo sapiens 164-171 18952566-6 2009 m(7)GTP-sepharose pulldown experiments showed a decrease in association of translation initiation factor, eIF4E, with its inhibitor, eIF4E-binding protein, and a concomitant increase in eIF4E association with eIF4G immediately after birth, and polysome profiles confirmed a decrease in abundance of large polysomes between FD19 and FD22, which was reversed on P1. Sepharose 8-17 eukaryotic translation initiation factor 4E Rattus norvegicus 106-111 18975072-4 2009 Purification by galactose-sepharose chromatography also indicated that the PPO and haemagglutinating activities were associated with a single protein. Sepharose 26-35 protoporphyrinogen oxidase Homo sapiens 75-78 19099209-3 2009 The scFv-Fc fusion protein, showing spontaneous Fc fragment-mediated homodimerization via disulfide bridges, was affinity-purified on protein A Sepharose from culture supernatant. Sepharose 144-153 immunglobulin heavy chain variable region Homo sapiens 4-8 20191190-2 2009 The heterogeneity of spa gene was determined directly by agarose gel electrophoresis migration. Sepharose 57-64 surfactant protein A2 Homo sapiens 21-24 19301208-4 2009 HK-2 cells were cultured in RPMI medium either on agarose gel or on plastic surface in order to inhibit or to allow cell proliferation. Sepharose 50-57 hexokinase 2 Homo sapiens 0-4 19151387-5 2009 METHODS: Fibronectin was purified from murine serum by gelatin cross-linked agarose chromatography and subsequently was enzymatically digested with alpha-chymotrypsin. Sepharose 76-83 fibronectin 1 Mus musculus 9-20 19601772-4 2009 About 15% of the GBF fraction"s IgG, compared to only about 0.3% of the NHS IgG, was affinity purified on IgG-Sepharose. Sepharose 110-119 Kruppel like factor 6 Homo sapiens 17-20 19700799-5 2009 METHODS: Recombinant baculovirus-expressed ZP3 was purified, labelled with biotin and coated on streptavidin sepharose beads. Sepharose 109-118 zona pellucida glycoprotein 3 Homo sapiens 43-46 19419969-1 2009 The rough endoplasmic reticulum-resident protein complex consisting of prolyl 3-hydroxylase 1 (P3H1), cartilage-associated protein (CRTAP), and cyclophilin B (CypB) can be isolated from chick embryos on a gelatin-Sepharose column, indicating some involvement in the biosynthesis of procollagens. Sepharose 213-222 peptidylprolyl isomerase B (cyclophilin B) Gallus gallus 159-163 19283727-6 2009 In this study, elastin tubes with agarose gel containing either stromal-derived factor-1 alpha [SDF; for homing of endothelial cells (ECs)] or basic fibroblast growth factor (bFGF; for homing of myofibroblasts) were implanted into adipose tissue, as it is a known source of stem/progenitor cells. Sepharose 34-41 elastin Homo sapiens 15-22 19726331-4 2009 The fusion protein GST-Id-2 expressed in E. coli following IPTG induction was purified by glutathione-agarose affinity chromatography and used to immunize rabbits to prepare the polyclonal antibodies against GST-Id-2. Sepharose 102-109 glutathione S-transferase kappa 1 Homo sapiens 19-22 19726331-4 2009 The fusion protein GST-Id-2 expressed in E. coli following IPTG induction was purified by glutathione-agarose affinity chromatography and used to immunize rabbits to prepare the polyclonal antibodies against GST-Id-2. Sepharose 102-109 DNA-binding protein inhibitor ID-2 Oryctolagus cuniculus 23-27 19321500-5 2009 LeishIF4G-3 was found to coelute with the parasite eIF4F subunits from an m(7)GTP-Sepharose column and to bind directly to LeishIF4E. Sepharose 82-91 eukaryotic translation initiation factor 4 gamma 1 Homo sapiens 51-56 19125587-4 2009 METHODS AND RESULTS: In sheep model, 1800 mL of blood was passed through a Sepharose-based column with affinity for CD133. Sepharose 75-84 prominin 1 Homo sapiens 116-121 18952566-6 2009 m(7)GTP-sepharose pulldown experiments showed a decrease in association of translation initiation factor, eIF4E, with its inhibitor, eIF4E-binding protein, and a concomitant increase in eIF4E association with eIF4G immediately after birth, and polysome profiles confirmed a decrease in abundance of large polysomes between FD19 and FD22, which was reversed on P1. Sepharose 8-17 eukaryotic translation initiation factor 4E Rattus norvegicus 133-138 18952566-6 2009 m(7)GTP-sepharose pulldown experiments showed a decrease in association of translation initiation factor, eIF4E, with its inhibitor, eIF4E-binding protein, and a concomitant increase in eIF4E association with eIF4G immediately after birth, and polysome profiles confirmed a decrease in abundance of large polysomes between FD19 and FD22, which was reversed on P1. Sepharose 8-17 eukaryotic translation initiation factor 4E Rattus norvegicus 133-138 18952566-6 2009 m(7)GTP-sepharose pulldown experiments showed a decrease in association of translation initiation factor, eIF4E, with its inhibitor, eIF4E-binding protein, and a concomitant increase in eIF4E association with eIF4G immediately after birth, and polysome profiles confirmed a decrease in abundance of large polysomes between FD19 and FD22, which was reversed on P1. Sepharose 8-17 eukaryotic translation initiation factor 4 gamma 1 Rattus norvegicus 209-214 19621046-4 2009 Immunosorbents were obtained by immobilization of the synthesizes antigens on the bromocyanogenactivated sepharose and applied to the removal of autoantibodies in a beta(1)-adrenoreceptor from the blood plasma of patients. Sepharose 105-114 adrenoceptor beta 1 Homo sapiens 165-187 19660048-4 2009 SAP binds to some types of agarose in the presence of Ca(2+). Sepharose 27-34 amyloid P component, serum Homo sapiens 0-3 19660048-5 2009 We found that human SAP binds to an agarose with a K(D) of 7 x 10(-8) M and a B(max) of 2.1 microg SAP/mg wet weight agarose. Sepharose 36-43 amyloid P component, serum Homo sapiens 20-23 19660048-5 2009 We found that human SAP binds to an agarose with a K(D) of 7 x 10(-8) M and a B(max) of 2.1 microg SAP/mg wet weight agarose. Sepharose 36-43 amyloid P component, serum Homo sapiens 99-102 19660048-5 2009 We found that human SAP binds to an agarose with a K(D) of 7 x 10(-8) M and a B(max) of 2.1 microg SAP/mg wet weight agarose. Sepharose 117-124 amyloid P component, serum Homo sapiens 20-23 19660048-5 2009 We found that human SAP binds to an agarose with a K(D) of 7 x 10(-8) M and a B(max) of 2.1 microg SAP/mg wet weight agarose. Sepharose 117-124 amyloid P component, serum Homo sapiens 99-102 19660048-6 2009 Mixing this agarose 1 : 5 w/v with 30 microg/mL human SAP (the average SAP concentration in normal serum) in a buffer containing 2 mM Ca(2+) reduced the free SAP concentration to approximately 0.02 microg/mL, well below the concentration that inhibits fibrocyte differentiation. Sepharose 12-19 amyloid P component, serum Homo sapiens 54-57 19660048-6 2009 Mixing this agarose 1 : 5 w/v with 30 microg/mL human SAP (the average SAP concentration in normal serum) in a buffer containing 2 mM Ca(2+) reduced the free SAP concentration to approximately 0.02 microg/mL, well below the concentration that inhibits fibrocyte differentiation. Sepharose 12-19 amyloid P component, serum Homo sapiens 71-74 19660048-6 2009 Mixing this agarose 1 : 5 w/v with 30 microg/mL human SAP (the average SAP concentration in normal serum) in a buffer containing 2 mM Ca(2+) reduced the free SAP concentration to approximately 0.02 microg/mL, well below the concentration that inhibits fibrocyte differentiation. Sepharose 12-19 amyloid P component, serum Homo sapiens 71-74 19282656-3 2009 The FVIII/VWF complex was purified from a healthy donor"s plasma by affinity chromatography on a Sepharose 4B-Concanavalin A column and was used to determine its capability to interact with erythrocytes and platelets. Sepharose 97-109 coagulation factor VIII Homo sapiens 4-9 19193840-1 2009 A trunk of human cytidylate-phosphate-deoxyguanylate-binding protein/CXXC finger protein 1 (CFP1), immobilized onto an aminohexyl-Sepharose column, can be used as a preanalytical tool for the selective enrichment of bacterial DNA from mixed solutions with high amounts of human background DNA for nucleic acid amplification technique-based detection of pathogens. Sepharose 130-139 CXXC finger protein 1 Homo sapiens 69-90 19193840-1 2009 A trunk of human cytidylate-phosphate-deoxyguanylate-binding protein/CXXC finger protein 1 (CFP1), immobilized onto an aminohexyl-Sepharose column, can be used as a preanalytical tool for the selective enrichment of bacterial DNA from mixed solutions with high amounts of human background DNA for nucleic acid amplification technique-based detection of pathogens. Sepharose 130-139 CXXC finger protein 1 Homo sapiens 92-96 19066403-7 2009 Agarose gel electrophoresis demonstrated the appearance of new pre-beta mobility fractions containing apoA-I and apoA-II in liposomes and HDL mixtures. Sepharose 0-7 apolipoprotein A1 Homo sapiens 102-108 19066403-7 2009 Agarose gel electrophoresis demonstrated the appearance of new pre-beta mobility fractions containing apoA-I and apoA-II in liposomes and HDL mixtures. Sepharose 0-7 apolipoprotein A2 Homo sapiens 113-120 19084045-0 2009 Characterisation of rat and human tissue alkaline phosphatase isoforms by high-performance liquid chromatography and agarose gel electrophoresis. Sepharose 117-124 alkaline phosphatase, placental Homo sapiens 41-61 19344517-11 2009 IgG-Sepharose affinity column chromatographic separation of Z domain containing chloroplast derived IGF-1 protein, single and two dimensional electrophoresis methods and mass spectrometer analysis confirmed the identity of human IGF-1 in transgenic chloroplasts. Sepharose 4-13 insulin like growth factor 1 Homo sapiens 229-234 19331430-1 2009 A novel fluorescent CdS-encapsulated DNA nanocomposite was synthesized via alternate adsorption of Cd(2+) and S(2-) onto the DNA template affixed inside an agarose gel. Sepharose 156-163 CDP-diacylglycerol synthase 1 Homo sapiens 20-23 19167525-2 2009 Recombinant rat Protease Nexin-1 (rPN-1) was efficiently produced in Escherichia coli using a T7 RNA polymerase based expression system and purified by heparin-sepharose affinity chromatography yielding 3 mg of protein per liter of cell culture. Sepharose 160-169 serpin family E member 2 Rattus norvegicus 16-32 19167525-2 2009 Recombinant rat Protease Nexin-1 (rPN-1) was efficiently produced in Escherichia coli using a T7 RNA polymerase based expression system and purified by heparin-sepharose affinity chromatography yielding 3 mg of protein per liter of cell culture. Sepharose 160-169 serpin family E member 2 Rattus norvegicus 34-39 19340922-1 2009 The purified electric eel acetylcholinesterase (AChE) was able to bind to the Zn+2-chelate-Sepharose affinity column only on treatment with EDTA. Sepharose 91-100 acetylcholinesterase Capra hircus 26-46 19340922-1 2009 The purified electric eel acetylcholinesterase (AChE) was able to bind to the Zn+2-chelate-Sepharose affinity column only on treatment with EDTA. Sepharose 91-100 acetylcholinesterase Capra hircus 48-52 19146949-4 2009 Herein we extensively purified recombinant rat TrxR1 over PAO Sepharose, which gave an enzyme with about 53 U/mg specific activity. Sepharose 62-71 thioredoxin reductase 1 Rattus norvegicus 47-52 19127344-6 2009 Inhibition of HGF binding to heparin by protamine was confirmed using heparin-coated sepharose. Sepharose 85-94 hepatocyte growth factor Rattus norvegicus 14-17 19136670-5 2009 Fast protein liquid chromatography analysis and agarose gel electrophoresis revealed that EL expression resulted in the generation of small pre-beta HDL particles in wild-type mice, whereas in SR-BI-/- mice small HDL were preferentially removed. Sepharose 48-55 lipase, endothelial Mus musculus 90-92 18937214-5 2009 The BChE from 1 ml of serum was rapidly purified on a 0.2 ml procainamide-Sepharose column. Sepharose 74-83 butyrylcholinesterase Homo sapiens 4-8 19282656-3 2009 The FVIII/VWF complex was purified from a healthy donor"s plasma by affinity chromatography on a Sepharose 4B-Concanavalin A column and was used to determine its capability to interact with erythrocytes and platelets. Sepharose 97-109 von Willebrand factor Homo sapiens 10-13 18996459-5 2009 The DNA binding and hydrolyzing activities of the recovered 3D8 scFv were evaluated by enzyme-linked immunosorbent assay (ELISA) and agarose gel electrophoresis, respectively. Sepharose 133-140 immunglobulin heavy chain variable region Homo sapiens 64-68 19173042-3 2009 With a sugar-boronic acid interaction, the AFP antigen could be effectively immobilized on the sepharose gel matrix. Sepharose 95-104 alpha fetoprotein Homo sapiens 43-46 19267679-5 2009 After cleavage of the thioredoxin/FGF-2 fusion with recombinant human enteropeptidase light chain, the target protein FGF-2 was purified on a heparin-Sepharose column. Sepharose 150-159 fibroblast growth factor 2 Homo sapiens 118-123 19317654-5 2009 The resulting RNAs are shown to be RNase A-resistant and capable of direct coupling to adipic acid dihydrazide agarose beads. Sepharose 111-118 ribonuclease A family member 1, pancreatic Homo sapiens 35-42 19378061-3 2009 The large pores of the agarose also allow full transfer of proteins as large as titin (Mr =3,000-3,700 kDa) onto blots. Sepharose 23-30 titin Homo sapiens 80-85 19363993-1 2009 Transthyretin (TTR) was previously called prealbumin because the band it formed on agarose gel electrophoresis at pH 8.6 was at the prealbumin position. Sepharose 83-90 transthyretin Homo sapiens 15-18 18923454-5 2009 On agarose gel electrophoresis analysis, six of eight ATMs showed trans-splicing efficacy when applied to DMPK mini-gene construct transcripts, of which three were able to trans-splice endogenous DMPK pre-mRNA transcripts in myosarcoma cells, with trans-splicing efficiency ranging from 1.81 to 7.41%. Sepharose 3-10 DM1 protein kinase Homo sapiens 106-110 18923454-5 2009 On agarose gel electrophoresis analysis, six of eight ATMs showed trans-splicing efficacy when applied to DMPK mini-gene construct transcripts, of which three were able to trans-splice endogenous DMPK pre-mRNA transcripts in myosarcoma cells, with trans-splicing efficiency ranging from 1.81 to 7.41%. Sepharose 3-10 DM1 protein kinase Homo sapiens 196-200 18779738-7 2009 The variable number of tandem repeats of IL-1RN was genotyped using polymerase chain reaction followed by agarose gel electrophoresis. Sepharose 106-113 interleukin 1 receptor antagonist Homo sapiens 41-47 19098486-3 2009 METHODS: HLA-G 14-bp deletion/insertion polymorphism (rs16375) was detected by PCR amplification of the target sequence followed by agarose gel electrophoresis. Sepharose 132-139 major histocompatibility complex, class I, G Homo sapiens 9-14 19036647-1 2009 Tris(2-aminoethyl)amine (TREN) - a chelating agent used in IMAC - immobilized onto agarose gel was evaluated for the purification of IgG from human serum by negative chromatography. Sepharose 83-90 C-C motif chemokine ligand 26 Homo sapiens 59-63 19378031-12 2009 GST-pulldown experiments are similar in principle to Co-IPs, but a bait GST-fusion protein complexed to glutathione-sepharose (GSH) beads is used to pull down interaction partners instead of an antibody. Sepharose 116-125 glutathione S-transferase kappa 1 Homo sapiens 72-75 19363993-1 2009 Transthyretin (TTR) was previously called prealbumin because the band it formed on agarose gel electrophoresis at pH 8.6 was at the prealbumin position. Sepharose 83-90 transthyretin Homo sapiens 0-13 18726690-9 2009 The isolation of the Galalpha1-6GlcNH(2) binding protein was performed by affinity chromatography (melibiose-agarose) and LC-MS/MS, and we identified the human heterogeneous ribonucleoprotein (hnRNP) A1 (34.3 kDa) isoform protein (30.8 kDa). Sepharose 109-116 heterogeneous nuclear ribonucleoprotein A1 Homo sapiens 160-202 18726690-10 2009 The hnRNP A1 protein was also detected from the eluate(s) of the MelNH-agarose column by the immunological method (anti-hnRNP-A1 and HRP-labeled anti-mouse IgG (gamma) antibodies). Sepharose 71-78 heterogeneous nuclear ribonucleoprotein A1 Homo sapiens 4-12 18984006-5 2009 By nine RT-PCRs simultaneously in a set of separate tubes, the subtype of NA was determined by subsequent agarose gel electrophoresis and ethidium bromide staining, since only one of the nine RT-PCRs would give a product of expected size for each virus strain. Sepharose 106-113 neuraminidase 1 Homo sapiens 74-76 19459332-2 2009 The GST-SOX4 soluble protein was expressed in Escherichia coli DH5alpha and purified by glutathione sepharose-4B. Sepharose 100-112 SRY (sex determining region Y)-box 4 Mus musculus 8-12 19190814-5 2009 The multimeric pattern of VWF was analyzed by SDS-agarose gel electrophoresis. Sepharose 50-57 von Willebrand factor Homo sapiens 26-29 20698171-7 2009 Intron 2 of the IL-1RN gene was studied using PCR and agarose gel electrophoresis. Sepharose 54-61 interleukin 1 receptor antagonist Homo sapiens 16-22 19191724-10 2009 In samples containing >10 nM haptocorrin (n=19), the median concentration of cobalamins decreased from 1.3 nM to 0.67 nM after pretreatment with cobinamide-sepharose. Sepharose 159-168 transcobalamin 1 Homo sapiens 32-43 19191724-12 2009 Removal of unsaturated haptocorrin by pretreatment with cobinamide-sepharose solves the problem. Sepharose 67-76 transcobalamin 1 Homo sapiens 23-34 19637058-5 2009 Employing recombinant TIMP-1 bound Sepharose beads and Western blots, we demonstrated binding between recombinant proMMP-2 and TIMP-1 proteins. Sepharose 35-44 TIMP metallopeptidase inhibitor 1 Homo sapiens 22-28 18399763-3 2009 Recent studies have demonstrated the capacity of unconfined cyclic compression to induce chondrogenic differentiation of human mesenchymal stem cell (hMSC) in agarose culture. Sepharose 159-166 musculin Homo sapiens 150-154 19739025-2 2009 Lysozyme and ovalbumin were separated with Q Sepharose Fast Flow anion exchange chromatography in the first step, resulting in two peaks of lysozyme and three peaks of ovalbumin with 87% and 70% purity by HPLC, respectively. Sepharose 45-54 lysozyme Homo sapiens 0-8 18975953-3 2008 OPV-Tyr was elaborated to detect tyrosinase activity both in aqueous buffer solution and in agarose gel. Sepharose 92-99 tyrosinase Homo sapiens 33-43 18627351-8 2008 MS analysis of the nucleoprotein complex unique to -156 to -203 bp after streptavidin-agarose pull-down detected the presence of the redox-active protein thioredoxin. Sepharose 86-93 Thioredoxin-like Drosophila melanogaster 154-165 18938157-0 2008 Increased stromal extracellular matrix synthesis and assembly by insulin activated bovine keratocytes cultured under agarose. Sepharose 117-124 insulin Bos taurus 65-72 19763506-3 2009 We have developed a novel approach to isolate large complexes of proteins associated with the DNA-bound estrogen receptor alpha (ERalpha) using an agarose-based electrophoretic mobility shift assay (EMSA). Sepharose 147-154 estrogen receptor 1 Homo sapiens 129-136 18726991-9 2008 Furthermore, AR proteolytic activity pulled down by calmodulin-agarose beads from celastrol-treated PC-3 cells showed immunoreactivity to a calpain antibody. Sepharose 63-70 androgen receptor Homo sapiens 13-15 18791061-6 2008 GSH and EdAG were equally effective in displacing a glutathione S-transferase (GST) isozyme (human GSTA1-1) from a GSH-agarose column. Sepharose 119-126 glutathione S-transferase alpha 1 Homo sapiens 99-106 18938157-7 2008 Agarose overlay of insulin activated keratocytes in culture is a useful model for studying corneal stromal extracellular matrix assembly in vitro. Sepharose 0-7 insulin Bos taurus 19-26 19114373-2 2008 METHOD: The recombinant expression plasmid pET14b-HMGB1 was constructed and transformed into competent E.coli BL21 cells to obtain HMGB1 protein, which was purified with chromatography on Ni-NTA Sepharose column. Sepharose 195-204 high mobility group box 1 Homo sapiens 131-136 19048125-6 2008 We used nickel agarose chromatin enrichment, chromatin immunoprecipitation, and the human embryonic kidney-derived cell line HEK293 to identify regulatory elements responding to PAX2. Sepharose 15-22 paired box 2 Homo sapiens 178-182 18418639-9 2008 LRP5 V667M was genotyped by allele-specific PCR and ESRRA repeats by sizing of PCR products on agarose gels. Sepharose 95-102 LDL receptor related protein 5 Homo sapiens 0-4 19074826-11 2008 Finally, affinity purification of beta(1)-integrin using rTF-conjugated agarose showed a requirement for the presence of divalent cations but not factor VIIa. Sepharose 72-79 integrin subunit beta 1 Homo sapiens 34-50 18687473-4 2008 The purified MBP-BF2-(G4S)3-beta(2)m protein was cleaved by Factor Xa protease, and further purified by DEAE-Sepharose chromatography. Sepharose 109-118 myelin basic protein Gallus gallus 13-16 18687473-4 2008 The purified MBP-BF2-(G4S)3-beta(2)m protein was cleaved by Factor Xa protease, and further purified by DEAE-Sepharose chromatography. Sepharose 109-118 Major histocompatibility complex class I antigen BF2 Gallus gallus 17-20 18775457-5 2008 As a result, using this GST:DEVD:EGFP reporter, caspase-3 activation based on proteolytic properties could be monitored via a variety of bioanalytical techniques such as immunoblot analysis, glutathione-agarose bead assay, and on-chip visualization, providing both technical and economical advantages over the extensively utilized fluorogenic peptide assay. Sepharose 203-210 glutathione S-transferase kappa 1 Homo sapiens 24-27 18775457-5 2008 As a result, using this GST:DEVD:EGFP reporter, caspase-3 activation based on proteolytic properties could be monitored via a variety of bioanalytical techniques such as immunoblot analysis, glutathione-agarose bead assay, and on-chip visualization, providing both technical and economical advantages over the extensively utilized fluorogenic peptide assay. Sepharose 203-210 caspase 3 Homo sapiens 48-57 18796530-10 2008 However, the organization of the vimentin had a radiate organization in chondrocytes in monolayer and a more circumferential arrangement both in agarose gel and in situ. Sepharose 145-152 vimentin Bos taurus 33-41 18384083-11 2008 Our experiments suggest that the dimeric CaM/CaMBD complex exists in solution, which is unambiguously validated using a carefully-designed CaM-sepharose pull-down experiment. Sepharose 143-152 calmodulin 1 Homo sapiens 41-44 18384083-11 2008 Our experiments suggest that the dimeric CaM/CaMBD complex exists in solution, which is unambiguously validated using a carefully-designed CaM-sepharose pull-down experiment. Sepharose 143-152 calmodulin 1 Homo sapiens 45-48 18602477-4 2008 The purification of Hu BChE was accomplished by batch adsorption on procainamide-Sepharose-CL-4B affinity gel followed by ion-exchange chromatography on a DEAE-Sepharose column. Sepharose 81-90 butyrylcholinesterase Homo sapiens 23-27 18603002-1 2008 A natural lectin from the plasma of the shrimp Fenneropenaeus chinensis was purified by singlestep affinity chromatography using fetuin-coupled agarose. Sepharose 144-151 ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Homo sapiens 0-1 18425992-8 2008 This observation was further confirmed by agarose gel electrophoresis, in which clear ladder-like DNA bands were observed from cells exposed to Pep1, whereas DNA from cells treated with mPep1 produced a smeared pattern. Sepharose 42-49 CNDP dipeptidase 2 (metallopeptidase M20 family) Mus musculus 144-148 18947830-3 2008 The adsorption equilibrium and kinetics of lysozyme and bovine serum albumin (BSA) to AI-Sepharose were determined by batch adsorption experiments at different conditions to provide insight into the adsorption properties of the medium. Sepharose 89-98 albumin Homo sapiens 63-76 18771724-8 2008 Moreover, using EGCG-treated cancer cells, we identified GAPDH as a target of EGCG covalent binding through specific interactions between catechols and aminophenyl boronate agarose resin. Sepharose 173-180 glyceraldehyde-3-phosphate dehydrogenase Homo sapiens 57-62 19000314-3 2008 RESULTS: We demonstrate that a peptide based on the RVXF/W motif can effectively displace PP1 bound proteins from PP1 retained on the phosphatase affinity matrix microcystin-Sepharose. Sepharose 174-183 neuropeptide Y receptor Y4 Homo sapiens 90-93 19000314-3 2008 RESULTS: We demonstrate that a peptide based on the RVXF/W motif can effectively displace PP1 bound proteins from PP1 retained on the phosphatase affinity matrix microcystin-Sepharose. Sepharose 174-183 neuropeptide Y receptor Y4 Homo sapiens 114-117 19000314-6 2008 CONCLUSION: This modification of the microcystin-Sepharose technique offers an effective means of purifying novel PP1 regulatory subunits and associated proteins and provides a simple method to uncover a link between PP1 and additional cellular processes. Sepharose 49-58 neuropeptide Y receptor Y4 Homo sapiens 114-117 19000314-6 2008 CONCLUSION: This modification of the microcystin-Sepharose technique offers an effective means of purifying novel PP1 regulatory subunits and associated proteins and provides a simple method to uncover a link between PP1 and additional cellular processes. Sepharose 49-58 neuropeptide Y receptor Y4 Homo sapiens 217-220 18712286-7 2008 The elution pattern of papain solubilized lactase from agarose-Wheat Germ agglutinin, or Concanavalin A or Jacalin agglutinin columns was different in the suckling and adult rat intestines. Sepharose 55-62 lactase Rattus norvegicus 42-49 18712286-8 2008 Also the elution profile of lactase activity from agarose-lectin columns was modulated in cortisone, thyroxine, and insulin injected pups, which suggests differences in glycosylated isoforms of lactase under these conditions. Sepharose 50-57 lactase Rattus norvegicus 28-35 18712286-8 2008 Also the elution profile of lactase activity from agarose-lectin columns was modulated in cortisone, thyroxine, and insulin injected pups, which suggests differences in glycosylated isoforms of lactase under these conditions. Sepharose 50-57 lactase Rattus norvegicus 194-201 18708147-8 2008 Three of the bands specifically retained on SMOC-Sepharose were identified as C-reactive protein, an acute phase protein from the pentraxin family, the basement membrane and elastic fiber-associated fibulin-1, and vitronectin, which is involved in cell adhesion, migration and proliferation and binds numerous extracellular and membrane proteins, including integrins. Sepharose 49-58 vitronectin Homo sapiens 214-225 18693254-8 2008 By affinity chromatographic analysis using anti-p57/coronin-1 antibody-conjugated Sepharose, p57/coronin-1 derived from PMA-treated HL60 cells showed lower affinity for actin than that from untreated cells. Sepharose 82-91 coronin 1A Homo sapiens 48-51 18693254-8 2008 By affinity chromatographic analysis using anti-p57/coronin-1 antibody-conjugated Sepharose, p57/coronin-1 derived from PMA-treated HL60 cells showed lower affinity for actin than that from untreated cells. Sepharose 82-91 coronin 1A Homo sapiens 52-61 18693254-8 2008 By affinity chromatographic analysis using anti-p57/coronin-1 antibody-conjugated Sepharose, p57/coronin-1 derived from PMA-treated HL60 cells showed lower affinity for actin than that from untreated cells. Sepharose 82-91 coronin 1A Homo sapiens 93-96 18693254-8 2008 By affinity chromatographic analysis using anti-p57/coronin-1 antibody-conjugated Sepharose, p57/coronin-1 derived from PMA-treated HL60 cells showed lower affinity for actin than that from untreated cells. Sepharose 82-91 coronin 1A Homo sapiens 97-106 18697746-5 2008 The C6ST-1 was expressed as a soluble protein A chimeric form in COS-1 cells and purified using IgG-Sepharose. Sepharose 100-109 carbohydrate sulfotransferase 3 Homo sapiens 4-10 18662664-6 2008 The binding of GST-Gal1(N46D) to asialofetuin-Sepharose was less than 10% of that observed for GST-Gal1(WT), indicating that the mutant was deficient in carbohydrate-binding activity. Sepharose 46-55 glutathione S-transferase kappa 1 Homo sapiens 15-18 18662664-6 2008 The binding of GST-Gal1(N46D) to asialofetuin-Sepharose was less than 10% of that observed for GST-Gal1(WT), indicating that the mutant was deficient in carbohydrate-binding activity. Sepharose 46-55 galectin 1 Homo sapiens 19-23 18635666-7 2008 Pyk2 was shown to bind specifically to a Cam agarose affinity column in a calcium-dependent manner, suggesting Cam and Pyk2 are capable of forming a complex. Sepharose 45-52 PTK2 protein tyrosine kinase 2 beta Mus musculus 0-4 18635666-7 2008 Pyk2 was shown to bind specifically to a Cam agarose affinity column in a calcium-dependent manner, suggesting Cam and Pyk2 are capable of forming a complex. Sepharose 45-52 calmodulin 2 Mus musculus 41-44 18635666-7 2008 Pyk2 was shown to bind specifically to a Cam agarose affinity column in a calcium-dependent manner, suggesting Cam and Pyk2 are capable of forming a complex. Sepharose 45-52 calmodulin 2 Mus musculus 111-114 18635666-7 2008 Pyk2 was shown to bind specifically to a Cam agarose affinity column in a calcium-dependent manner, suggesting Cam and Pyk2 are capable of forming a complex. Sepharose 45-52 PTK2 protein tyrosine kinase 2 beta Mus musculus 119-123 20300302-5 2008 The apo E gene polymorphisms were determined by agarose gel electrophoresis. Sepharose 48-55 apolipoprotein E Homo sapiens 4-9 18511454-4 2008 FGF2, bound to Heparin Sepharose, could be eluted with ATP and GTP, but not with cAMP, AMP or ADP. Sepharose 23-32 fibroblast growth factor 2 Homo sapiens 0-4 18611412-5 2008 The expressed VP7 was purified to near homogeneity by chromatography on nickel-agarose column as judged by sodium dodesyl sulfate-polyacrylamide gel electrophoresis analysis. Sepharose 79-86 VP7 Bluetongue virus 14-17 18620053-9 2008 Using an HA-nucleation assay, it was shown that rBSP is ten-fold more potent in reconstituted fibrillar collagen gels than in agarose gels. Sepharose 126-133 integrin-binding sialoprotein Rattus norvegicus 48-52 18174267-3 2008 METHODS: The lysosomal enzyme cathepsin D was measured by ELISA and isolated by pepstatin-agarose affinity chromatography; N-acetyl-beta-d-glucosaminidase (NAG) was assayed colorimetrically, as was the cytosolic enzyme lactate dehydrogenase (LDH). Sepharose 90-97 cathepsin D Homo sapiens 30-41 18174267-6 2008 Soluble CI-MPR, isolated from fetal calf serum and bound to agarose, was used to probe cathepsin D for mannose-6-phosphate (M6P). Sepharose 60-67 insulin like growth factor 2 receptor Homo sapiens 8-14 18174267-11 2008 Cathepsin D binding to CI-MPR-agarose was inhibited by M6P. Sepharose 30-37 cathepsin D Homo sapiens 0-11 18174267-11 2008 Cathepsin D binding to CI-MPR-agarose was inhibited by M6P. Sepharose 30-37 insulin like growth factor 2 receptor Homo sapiens 23-29 18323737-11 2008 When HSCM and CM were subjected to Hsp72 depletion via adenosine triphosphate-agarose binding, LPS-mediated activation of NF-kappaB was partially restored, suggesting that Hsp72 is partially responsible for cellular reprogramming in response to HSCM. Sepharose 78-85 heat shock protein family A (Hsp70) member 1A Homo sapiens 35-40 18323737-11 2008 When HSCM and CM were subjected to Hsp72 depletion via adenosine triphosphate-agarose binding, LPS-mediated activation of NF-kappaB was partially restored, suggesting that Hsp72 is partially responsible for cellular reprogramming in response to HSCM. Sepharose 78-85 nuclear factor kappa B subunit 1 Homo sapiens 122-131 18323737-11 2008 When HSCM and CM were subjected to Hsp72 depletion via adenosine triphosphate-agarose binding, LPS-mediated activation of NF-kappaB was partially restored, suggesting that Hsp72 is partially responsible for cellular reprogramming in response to HSCM. Sepharose 78-85 heat shock protein family A (Hsp70) member 1A Homo sapiens 172-177 18766256-3 2008 Following protein refolding and purification on lysine-Sepharose, the conversion of the recombinant molecule Delta(K2-K5)Pg to the active enzyme mutant Delta(K2-K5)Pm by plasminogen activators was evaluated, and functional characteristics of the simplified plasmin were studied. Sepharose 55-64 plasminogen Homo sapiens 170-177 18782522-4 2008 The expressed GST-ficolin-A fusion protein was purified via GST-Sepharose 4B Column and identified by SDS-PAGE and Western blot. Sepharose 64-73 ficolin A Mus musculus 18-27 18636177-5 2008 Agarose gel electrophoresis showed the presence of low-molecular weight deoxyribonucleic acid (DNA) fragments of adherent cells cultured with CRP (10(-7) M) plus zinc (10(-5) M) for 24 or 72 h in the presence of M-CSF and RANKL, indicating that the combination of the two chemicals induces apoptotic cell death. Sepharose 0-7 C-reactive protein, pentraxin-related Mus musculus 142-145 18636177-5 2008 Agarose gel electrophoresis showed the presence of low-molecular weight deoxyribonucleic acid (DNA) fragments of adherent cells cultured with CRP (10(-7) M) plus zinc (10(-5) M) for 24 or 72 h in the presence of M-CSF and RANKL, indicating that the combination of the two chemicals induces apoptotic cell death. Sepharose 0-7 colony stimulating factor 1 (macrophage) Mus musculus 212-217 18636177-5 2008 Agarose gel electrophoresis showed the presence of low-molecular weight deoxyribonucleic acid (DNA) fragments of adherent cells cultured with CRP (10(-7) M) plus zinc (10(-5) M) for 24 or 72 h in the presence of M-CSF and RANKL, indicating that the combination of the two chemicals induces apoptotic cell death. Sepharose 0-7 tumor necrosis factor (ligand) superfamily, member 11 Mus musculus 222-227 18550736-5 2008 The sensitivities of both VR-1 and VR-2 VZV LAMP determined by either the turbidity assay or agarose gel electrophoresis were 100 copies per reaction. Sepharose 93-100 vault RNA 1-1 Homo sapiens 26-48 18479936-3 2008 We show herein a method of overexpression and purification of two small specific domains corresponding to the isoforms b and c of the murine transcription factor Pitx2, and the generation and purification of monospecific polyclonal antibodies against them, by using a two-step affinity purification procedure, based on the use of CNBr-Sepharose matrix. Sepharose 335-344 paired-like homeodomain transcription factor 2 Mus musculus 162-167 18545934-0 2008 Signal transduction pathways involving p38 MAPK, JNK, NFkappaB and AP-1 influences the response of chondrocytes cultured in agarose constructs to IL-1beta and dynamic compression. Sepharose 124-131 mitogen-activated protein kinase 14 Bos taurus 39-42 18448074-8 2008 Unlike the mammalian homologue, DrGRIFIN contains all amino acids critical for binding to carbohydrate ligands and its activity was confirmed as the recombinant DrGRIFIN could be purified to homogeneity by affinity chromatography on a lactosyl-Sepharose column. Sepharose 244-253 galectin-related inter-fiber protein Danio rerio 32-40 18448074-8 2008 Unlike the mammalian homologue, DrGRIFIN contains all amino acids critical for binding to carbohydrate ligands and its activity was confirmed as the recombinant DrGRIFIN could be purified to homogeneity by affinity chromatography on a lactosyl-Sepharose column. Sepharose 244-253 galectin-related inter-fiber protein Danio rerio 161-169 18545934-0 2008 Signal transduction pathways involving p38 MAPK, JNK, NFkappaB and AP-1 influences the response of chondrocytes cultured in agarose constructs to IL-1beta and dynamic compression. Sepharose 124-131 Jun proto-oncogene, AP-1 transcription factor subunit Bos taurus 67-71 18545934-0 2008 Signal transduction pathways involving p38 MAPK, JNK, NFkappaB and AP-1 influences the response of chondrocytes cultured in agarose constructs to IL-1beta and dynamic compression. Sepharose 124-131 interleukin 1 beta Bos taurus 146-154 18344410-10 2008 Lipase activity detected in a 1.6 M NaCl-eluted fraction from a heparin-Sepharose column was enhanced by adding purified apoC-II in a dose-dependent manner, whereas that eluted by 0.8 M NaCl was not. Sepharose 72-81 apolipoprotein C2 Homo sapiens 121-128 18480459-4 2008 By adopting a native agarose gel electrophoresis assay that can specifically measure the levels of A3G incorporation into HBV nucleocapsids, we found that A3G is specifically packaged into replication-competent HBV nucleocapsids in a fashion that is dependent on both the viral reverse transcriptase (RT) and viral RNA packaging signal, epsilon. Sepharose 21-28 apolipoprotein B mRNA editing enzyme catalytic subunit 3G Homo sapiens 155-158 18234205-5 2008 We identified two characteristic binding sites of lysozyme on SP Sepharose Fast Flow and one multipoint interaction of lysozyme with SP Sepharose XL. Sepharose 65-74 lysozyme Homo sapiens 50-58 18467524-6 2008 Furthermore, we show that Thr256/Thr259 dephosphorylation is blocked by the protein phosphatase 2A (PP2A) inhibitor, okadaic acid, and demonstrate interactions between MAP2D and PP2A by coimmunoprecipitation and microcystin-agarose pull-down. Sepharose 224-231 protein phosphatase 2 phosphatase activator Homo sapiens 100-104 18467524-6 2008 Furthermore, we show that Thr256/Thr259 dephosphorylation is blocked by the protein phosphatase 2A (PP2A) inhibitor, okadaic acid, and demonstrate interactions between MAP2D and PP2A by coimmunoprecipitation and microcystin-agarose pull-down. Sepharose 224-231 protein phosphatase 2 phosphatase activator Homo sapiens 178-182 18756878-5 2008 The genotyping of polymorphism in the intron 16 of the angiotensin-converting enzyme was performed by the polymerase chain reaction followed by the agarose electrophoresis. Sepharose 148-155 angiotensin I converting enzyme Homo sapiens 55-84 18325077-4 2008 IgE antibodies were depleted from serum using sepharose-coupled mAb12 and IgE-bearing cells were enriched from heparinized blood samples with mAb12. Sepharose 46-55 immunoglobulin heavy constant epsilon Homo sapiens 0-3 18522879-7 2008 Agarose gel analysis of RT-PCR products indicated the presence of CTLA4Ig in the pSNAV-CTLA4Ig treatment group. Sepharose 0-7 cytotoxic T-lymphocyte-associated protein 4 Rattus norvegicus 66-71 18691141-4 2008 This protein is a 200-kDa molecule isolated by affinity chromatography using MAIP-1 (monoclonal antibody which recognizes PAS-1), coupled to Sepharose 4B. Sepharose 141-150 pulmonary adenoma susceptibility 1 Mus musculus 122-127 19130799-5 2008 ACE insertion/deletion polymorphism was determined by agarose gel electrophoresis and D/D typing was further reconfirmed using insertion-allele-specific amplification. Sepharose 54-61 angiotensin I converting enzyme Homo sapiens 0-3 17729254-1 2008 Agarose gel membranes (AGMs), which could selectively recognize bovine serum albumin (BSA) and bovine hemoglobin (Hb), were prepared by molecular imprinting technique under moderate preparation conditions. Sepharose 0-7 albumin Bos taurus 71-84 18375953-5 2008 Here, we show that the previously characterized prelatent antithrombin is a mixture of native antithrombin and a modified, true prelatent antithrombin that are resolvable by heparin-agarose chromatography. Sepharose 182-189 serpin family C member 1 Homo sapiens 58-70 18569282-5 2008 The target cells were exposed to LHRH-PE40 and its cytotoxicity was analyzed by scanning and transmission electron microscopies, agarose gel electrophoresis, and flow cytometry. Sepharose 129-136 gonadotropin releasing hormone 1 Homo sapiens 33-37 18291605-0 2008 Preparation and characterization of mucinated agarose: a mucin-agarose physical crosslink. Sepharose 46-53 LOC100508689 Homo sapiens 36-41 18624397-5 2008 The O-linked structures were found to be attached to the high molecular mass mucins isolated by agarose-polyacrylamide composite gel electrophoresis, where MUC1 and MUC4 were present. Sepharose 96-103 mucin 1, cell surface associated Homo sapiens 156-160 18624397-5 2008 The O-linked structures were found to be attached to the high molecular mass mucins isolated by agarose-polyacrylamide composite gel electrophoresis, where MUC1 and MUC4 were present. Sepharose 96-103 mucin 4, cell surface associated Homo sapiens 165-169 18291605-0 2008 Preparation and characterization of mucinated agarose: a mucin-agarose physical crosslink. Sepharose 63-70 LOC100508689 Homo sapiens 36-41 18291605-2 2008 Mucinated agarose, a physical crosslink of mucin and agarose, which are both biodegradable natural polymers, has been successfully prepared by a temperature controlled coarcervation technique of aqueous dispersions of equal concentrations of both polymers. Sepharose 10-17 LOC100508689 Homo sapiens 43-48 18291605-7 2008 The mucinated agarose showed characteristic swelling, mucoadhesiveness, moisture uptake and DSC thermal properties that were different from those of mucin and agarose alone. Sepharose 14-21 LOC100508689 Homo sapiens 4-9 18291605-7 2008 The mucinated agarose showed characteristic swelling, mucoadhesiveness, moisture uptake and DSC thermal properties that were different from those of mucin and agarose alone. Sepharose 159-166 LOC100508689 Homo sapiens 4-9 18291605-8 2008 The results indicated that there was formation of a crosslink between mucin and agarose. Sepharose 80-87 LOC100508689 Homo sapiens 70-75 18192592-8 2008 Fluorescent agarose casting also showed markedly decreased density of pulmonary arteries and artery filling defects in Cav1(-/-) mice. Sepharose 12-19 caveolin 1, caveolae protein Mus musculus 119-123 18284919-3 2008 Considering that abnormal regulation of DNA replication is linked to transformation, we examined whether the over-expression of fad24 leads to the formation of colonies in soft agarose. Sepharose 177-184 NOC3 like DNA replication regulator Homo sapiens 128-133 18422780-8 2008 Anti-FVIII IgGs were significantly captured by the peptide-Sepharose affinity matrixes as assessed by enzyme-linked immunosorbent assay. Sepharose 59-68 coagulation factor VIII Homo sapiens 5-10 18289663-3 2008 Net-positively-charged Lys (pI=11) and net-negatively-charged HSA (pI=5.5) adsorb so strongly to sulfopropyl sepharose (SP; a negatively-charged, strong cation-exchange resin, -0.22 mmol/mL exchange capacity) that both resist displacement by net-neutral IgG (pI=7.0) in simultaneous adsorption competition experiments. Sepharose 120-122 lysozyme Homo sapiens 23-26 18491301-3 2008 The GST fusion protein is easily purified by affinity chromatography using a glutathione-Sepharose matrix under mild conditions. Sepharose 89-98 glutathione S-transferase kappa 1 Homo sapiens 4-7 18424242-0 2008 Identification of the heparin-binding domain of TNF-alpha and its use for efficient TNF-alpha purification by heparin-Sepharose affinity chromatography. Sepharose 118-127 tumor necrosis factor Homo sapiens 48-57 18424242-0 2008 Identification of the heparin-binding domain of TNF-alpha and its use for efficient TNF-alpha purification by heparin-Sepharose affinity chromatography. Sepharose 118-127 tumor necrosis factor Homo sapiens 84-93 18424242-1 2008 The N-terminus of the trimeric TNF-alpha molecule comprises two basic arginines within the short amino-acid sequence VRSSSR, which is here shown to be essential for binding of TNF-alpha to heparin-Sepharose. Sepharose 197-206 tumor necrosis factor Homo sapiens 31-40 18424242-1 2008 The N-terminus of the trimeric TNF-alpha molecule comprises two basic arginines within the short amino-acid sequence VRSSSR, which is here shown to be essential for binding of TNF-alpha to heparin-Sepharose. Sepharose 197-206 tumor necrosis factor Homo sapiens 176-185 18424242-4 2008 Affinity chromatography on heparin-Sepharose was introduced as a key step for highly purified TNF-alpha at a high yield. Sepharose 35-44 tumor necrosis factor Homo sapiens 94-103 18424734-6 2008 Serum derived from MASP1/3(-/-) mice showed significantly lower activity of both C4 and C3 deposition on mannan-agarose, and this low activity was restored by the addition of recombinant MASP-1. Sepharose 112-119 mannan-binding lectin serine peptidase 1 Mus musculus 19-26 18424734-6 2008 Serum derived from MASP1/3(-/-) mice showed significantly lower activity of both C4 and C3 deposition on mannan-agarose, and this low activity was restored by the addition of recombinant MASP-1. Sepharose 112-119 mannan-binding lectin serine peptidase 1 Mus musculus 187-193 18343499-5 2008 Secretion of Ficolin-1 was investigated in cells and plasma from healthy donors through affinity purification using N-acetyl-d-glucosamine-agarose beads and ELISA. Sepharose 139-146 ficolin 1 Homo sapiens 13-22 18466700-4 2008 The ferment cell line of the recombinant sCR1 which was chosen by G418 resistance was identified by PCR, After methanol induction, the expressed protein products were verified by SDS-PAGE and Western blot, purified by Ni(2+)-NTA agarose affinity chromatography, and its biologic activity was identified. Sepharose 229-236 SCR1 Saccharomyces cerevisiae S288C 41-45 18466700-8 2008 The highly purified sCR1 fusion protein and its biologic activity were detected obtained by Ni(2+)-NTA agarose affinity chromatography. Sepharose 103-110 SCR1 Saccharomyces cerevisiae S288C 20-24 18446053-3 2008 The ability of the proteins to form in vitro protein-DNA complexes was analyzed on agarose gel; both GST-Hoxc8(1-242) and GST-Hoxc8(149-208) formed complexes. Sepharose 83-90 glutathione S-transferase kappa 1 Homo sapiens 101-104 18953870-5 2008 In vitro, the biology effect of WWOX on HO8910 cell was analyzed through the methyl thiazolyl tetrazolium test, transwell chamber cell invasion assay in vitro, agarose clony-formation and flow cytometry. Sepharose 160-167 WW domain containing oxidoreductase Homo sapiens 32-36 18279654-2 2008 We demonstrate that degradation due to non-specific cleavage of recombinant protein mediated by thrombin can be completely prevented by separation of thrombin from the recombinant protein on spin columns packed with heparin-sepharose. Sepharose 224-233 coagulation factor II, thrombin Homo sapiens 96-104 18279654-2 2008 We demonstrate that degradation due to non-specific cleavage of recombinant protein mediated by thrombin can be completely prevented by separation of thrombin from the recombinant protein on spin columns packed with heparin-sepharose. Sepharose 224-233 coagulation factor II, thrombin Homo sapiens 150-158 18446053-3 2008 The ability of the proteins to form in vitro protein-DNA complexes was analyzed on agarose gel; both GST-Hoxc8(1-242) and GST-Hoxc8(149-208) formed complexes. Sepharose 83-90 homeobox C8 Homo sapiens 126-131 18446053-3 2008 The ability of the proteins to form in vitro protein-DNA complexes was analyzed on agarose gel; both GST-Hoxc8(1-242) and GST-Hoxc8(149-208) formed complexes. Sepharose 83-90 homeobox C8 Homo sapiens 105-110 23105737-6 2008 For APOB XbaI polymorphism PCR product was digested with XbaI restriction enzyme, followed by agarose gel electrophoresis. Sepharose 94-101 apolipoprotein B Homo sapiens 4-8 18595521-5 2008 RT-PCR showed that mRNA expressions of TLR9 were found in primary nasal epithelial cells by 1% agarose gel electrophoresis. Sepharose 95-102 toll like receptor 9 Homo sapiens 39-43 18311572-3 2008 The mMDH was expressed in Escherichia coli as the recombinant protein with a GST tag and purified by glutathione-Sepharose 4B column. Sepharose 113-122 malate dehydrogenase 2, NAD (mitochondrial) Mus musculus 4-8 18171623-9 2008 hPRR-wTM was purified from the solubilized fraction using anti-FLAG M2 antibody agarose. Sepharose 80-87 ATPase H+ transporting accessory protein 2 Homo sapiens 0-4 18222932-2 2008 Sex identification using the intron length difference between W and Z chromosomal CHD1 genes, as visualized by agarose gel electrophoreses, often produces ambiguous results. Sepharose 111-118 chromodomain helicase DNA binding protein 1 Homo sapiens 82-86 18211894-6 2008 Microcystin-agarose pull-downs suggested that a phosphatase binds to FOXO1, and PP2A catalytic subunit was identified in endogenous FOXO1 immunocomplexes, indicating that PP2A is a FOXO1 phosphatase. Sepharose 12-19 forkhead box O1 Homo sapiens 69-74 18211894-6 2008 Microcystin-agarose pull-downs suggested that a phosphatase binds to FOXO1, and PP2A catalytic subunit was identified in endogenous FOXO1 immunocomplexes, indicating that PP2A is a FOXO1 phosphatase. Sepharose 12-19 protein phosphatase 2 phosphatase activator Homo sapiens 171-175 18031286-4 2008 Calmodulin-agarose bound CAKbeta/PYK2, but not FAK, in the presence of CaCl2. Sepharose 11-18 calmodulin 1 Homo sapiens 0-10 18031286-4 2008 Calmodulin-agarose bound CAKbeta/PYK2, but not FAK, in the presence of CaCl2. Sepharose 11-18 protein tyrosine kinase 2 beta Homo sapiens 25-32 18031286-4 2008 Calmodulin-agarose bound CAKbeta/PYK2, but not FAK, in the presence of CaCl2. Sepharose 11-18 protein tyrosine kinase 2 beta Homo sapiens 33-37 18311789-12 2008 In addition, TGFbeta-induced chondrocyte proliferation, chondrocyte cloning in agarose gel culture, and digestion of IGFBP-5 were inhibited with ADAM inhibitor, anti-ADAM-12 antibody, and small interfering RNA for ADAM-12. Sepharose 79-86 transforming growth factor beta 1 Homo sapiens 13-20 18460740-2 2008 In order to better understand this protein, galectin-3 from papillary thyroid carcinoma was partially purified by affinity chromatography on lactose-agarose. Sepharose 149-156 galectin 3 Homo sapiens 44-54 18036696-4 2008 We demonstrated by affinity chromatography that HIV-1 p17 binds strongly to heparin-agarose at physiological pH. Sepharose 84-91 family with sequence similarity 72 member B Homo sapiens 54-57 18179643-6 2008 The new method simplifies the analysis of FCGR3A and FCGR2A because the two alleles of each gene are detected simultaneously in a single reaction and separated, with no further manipulations, by their different electrophoretic mobilities in regular agarose gels. Sepharose 249-256 Fc gamma receptor IIIa Homo sapiens 42-48 18179643-6 2008 The new method simplifies the analysis of FCGR3A and FCGR2A because the two alleles of each gene are detected simultaneously in a single reaction and separated, with no further manipulations, by their different electrophoretic mobilities in regular agarose gels. Sepharose 249-256 Fc gamma receptor IIa Homo sapiens 53-59 18031558-3 2008 We sought further insight into l-ficolin binding reactions and MASP-2 activation by passing plasma through GlcNAc-derivatized Sepharose. Sepharose 126-135 MBL associated serine protease 2 Homo sapiens 63-69 18328185-3 2008 The recombinant protein hCD1d-alpha3 was purified with Ni(2+)-NTA agarose column and used as immunogen to immunize the rabbit. Sepharose 66-73 CD1d molecule Homo sapiens 24-29 18078772-4 2008 We developed an agarose-based porous media that is covalently spin-labeled with stable radicals. Sepharose 16-23 spindlin 1 Homo sapiens 62-66 17890383-11 2008 Agarose gel electrophoresis studies demonstrated that the aggregation induced by SP-B blocked migration of PG-coated NP. Sepharose 0-7 surfactant protein B Homo sapiens 81-85 18006516-6 2008 Pull-down assays using heparin-agarose gel show that heparin directly interacts with ACA8. Sepharose 31-38 autoinhibited Ca2+ -ATPase Arabidopsis thaliana 85-89 18006516-7 2008 Binding to the heparin-agarose gel occurs also with a peptide reproducing ACA8 sequence (1)M-I(116). Sepharose 23-30 autoinhibited Ca2+ -ATPase Arabidopsis thaliana 74-78 17986219-5 2008 For this, we analyzed samples from in vitro and in vivo Huntington"s Disease models by agarose gel electrophoresis and showed that in the brain of transgenic mice huntingtin-aggregates became larger as a function of disease progression. Sepharose 87-94 huntingtin Mus musculus 163-173 17653084-7 2008 Affinity chromatography on m(7)GTP-Sepharose indicates that the larger forms of 4E-BP1 bind very poorly to eIF4E. Sepharose 35-44 eukaryotic translation initiation factor 4E binding protein 1 Homo sapiens 80-86 18048363-5 2008 We found that intact EGFR was required if H-Ras-transformed cells were to grow in the absence of exogenous growth factors or form large colonies in agarose. Sepharose 148-155 epidermal growth factor receptor Homo sapiens 21-25 18048363-5 2008 We found that intact EGFR was required if H-Ras-transformed cells were to grow in the absence of exogenous growth factors or form large colonies in agarose. Sepharose 148-155 HRas proto-oncogene, GTPase Homo sapiens 42-47 18048363-6 2008 When we decreased expression of Spry2, using a Spry2-specific shRNA, the H-Ras(V12)-transformed fibroblasts could no longer form large colonies in agarose, grow in reduced levels of serum, or form tumors in athymic mice. Sepharose 147-154 sprouty RTK signaling antagonist 2 Mus musculus 32-37 18048363-6 2008 When we decreased expression of Spry2, using a Spry2-specific shRNA, the H-Ras(V12)-transformed fibroblasts could no longer form large colonies in agarose, grow in reduced levels of serum, or form tumors in athymic mice. Sepharose 147-154 Harvey rat sarcoma virus oncogene Mus musculus 73-78 17945191-6 2008 COX-1 in platelet lysates or isolated COX-1 selectively bound to an affinity matrix composed of immobilized BAs linked via glutaric acid to sepharose and this binding was reversed by ibuprofen or AA. Sepharose 140-149 mitochondrially encoded cytochrome c oxidase I Homo sapiens 0-5 18078818-3 2008 We developed hCG-coated agarose beads that mimic the physical and physiological features of an implantation-stage human blastocyst. Sepharose 24-31 chorionic gonadotropin subunit beta 5 Homo sapiens 13-16 18082144-0 2008 Arabidopsis HY5 protein functions as a DNA-binding tag for purification and functional immobilization of proteins on agarose/DNA microplate. Sepharose 117-124 Basic-leucine zipper (bZIP) transcription factor family protein Arabidopsis thaliana 12-15 17945191-6 2008 COX-1 in platelet lysates or isolated COX-1 selectively bound to an affinity matrix composed of immobilized BAs linked via glutaric acid to sepharose and this binding was reversed by ibuprofen or AA. Sepharose 140-149 mitochondrially encoded cytochrome c oxidase I Homo sapiens 38-43 18180850-10 2008 Furthermore, affinity chromatography of platelet proteins on an NA-LAAO-Sepharose 4B column isolated a few platelet membrane proteins, suggesting that binding of NA-LAAO to the platelet membrane might play a role in its action on platelets. Sepharose 72-84 interleukin 4 induced 1 Homo sapiens 67-71 18180850-10 2008 Furthermore, affinity chromatography of platelet proteins on an NA-LAAO-Sepharose 4B column isolated a few platelet membrane proteins, suggesting that binding of NA-LAAO to the platelet membrane might play a role in its action on platelets. Sepharose 72-84 interleukin 4 induced 1 Homo sapiens 165-169 18290319-14 2008 Immuno-affinity purification of FVL from patient plasma was then performed using an anti-FV light chain antibody immobilized CNBr-Sepharose, and the purification yield was 25%. Sepharose 130-139 coagulation factor V Homo sapiens 32-35 18290319-14 2008 Immuno-affinity purification of FVL from patient plasma was then performed using an anti-FV light chain antibody immobilized CNBr-Sepharose, and the purification yield was 25%. Sepharose 130-139 coagulation factor V Homo sapiens 32-34 17852554-3 2008 From quantification of the conformational changes of the irradiated samples (closed circular, open or linear forms) analyzed by agarose gel electrophoresis, the yields of SSB and DSB were obtained. Sepharose 128-135 small RNA binding exonuclease protection factor La Homo sapiens 171-174 18348730-0 2008 Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs. Sepharose 129-136 interleukin 1 beta Homo sapiens 32-40 18348730-0 2008 Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs. Sepharose 129-136 nitric oxide synthase 2 Homo sapiens 49-80 18348730-0 2008 Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs. Sepharose 129-136 prostaglandin-endoperoxide synthase 2 Homo sapiens 85-102 18585473-3 2008 PHEX was eluted from a heparin-Sepharose chromatography column at 0.8 M NaCl showing a strong interaction with heparin. Sepharose 31-40 phosphate regulating endopeptidase homolog X-linked Homo sapiens 0-4 19017475-1 2008 We report here anisotropic hydroxyapatite (HAp) formation inside an agarose gel using an electrophoretic approach and an alternate soaking process. Sepharose 68-75 reticulon 3 Homo sapiens 43-46 18756387-0 2008 Physicochemical, morphological and therapeutic evaluation of agarose hydrogel particles as a reservoir for basic fibroblast growth factor. Sepharose 61-68 fibroblast growth factor 2 Homo sapiens 107-137 17996248-3 2008 In the present study, the epitope-mapped monoclonal antibody CS9 was coupled to Sepharose beads and used as an affinity matrix for single-step immunoaffinity purification of Cyt b. Sepharose 80-89 mitochondrially encoded cytochrome b Homo sapiens 174-179 17996248-4 2008 Following solubilization of both human neutrophil and PLB-985 membrane fractions in the nonionic detergent octylglucoside, Cyt b was absorbed on the CS9-Sepharose affinity matrix and purified protein was eluted under non-denaturing conditions with an epitope-mimicking peptide. Sepharose 153-162 mitochondrially encoded cytochrome b Homo sapiens 123-128 18237253-9 2008 The protein A-agarose and ConA-sepharose affinity chromatography was applied to the purification of IgG, ConA-positive IgG, and Fab fragments. Sepharose 14-21 FA complementation group B Homo sapiens 128-131 18237253-9 2008 The protein A-agarose and ConA-sepharose affinity chromatography was applied to the purification of IgG, ConA-positive IgG, and Fab fragments. Sepharose 31-40 FA complementation group B Homo sapiens 128-131 19048102-6 2008 Streptavidin agarose column chromatography with the SBP tag successfully isolated the protein complexes in a native form with a high purity. Sepharose 13-20 selenium binding protein 1 Homo sapiens 52-55 18756387-1 2008 Micron-sized agarose hydrogel particles were prepared using an emulsification/gelation method as a controlled release reservoir for basic fibroblast growth factor (bFGF). Sepharose 13-20 fibroblast growth factor 2 Homo sapiens 132-162 18756387-1 2008 Micron-sized agarose hydrogel particles were prepared using an emulsification/gelation method as a controlled release reservoir for basic fibroblast growth factor (bFGF). Sepharose 13-20 fibroblast growth factor 2 Homo sapiens 164-168 18756387-8 2008 The therapeutic efficacy of bFGF retained in agarose hydrogel particles was significantly higher than that dissolved in saline. Sepharose 45-52 fibroblast growth factor 2 Homo sapiens 28-32 18756387-9 2008 Agarose hydrogel particle seems to be a potential candidate for a bFGF reservoir. Sepharose 0-7 fibroblast growth factor 2 Homo sapiens 66-70 17893094-4 2007 Expression of galectin-8 was verified at the protein level in total organ extracts by western-blots of lactosyl-Sepharose purified binders. Sepharose 112-121 lectin, galactose binding, soluble 8 Mus musculus 14-24 18855760-2 2008 The soluble fusion protein MBP-Gly1-Gln26-rhPTH(1-34) was harvested after purification by Phenyl-Sepharose F.F and Q-Sepharose F.F chromatographies. Sepharose 97-106 myelin basic protein Homo sapiens 27-30 18245907-5 2008 RESULTS: The ApoM gene was cloned by PCR and a 560 bp DNA fragment was shown on the agarose electrophoresis. Sepharose 84-91 apolipoprotein M Homo sapiens 13-17 18044967-10 2007 In addition, Src family kinases colocalized with activated Rac1 and with laminin-Sepharose in solid-phase binding assays. Sepharose 81-90 SRC proto-oncogene, non-receptor tyrosine kinase Rattus norvegicus 13-16 17854853-3 2007 In the present study, a unique LAO from Agkistrodon blomhoffii ussurensis snake venom named ABU-LAO was purified by Heparin-Sepharose FF chromatography followed by an ion-exchange chromatography procedure. Sepharose 124-133 interleukin 4 induced 1 Homo sapiens 31-34 17854853-3 2007 In the present study, a unique LAO from Agkistrodon blomhoffii ussurensis snake venom named ABU-LAO was purified by Heparin-Sepharose FF chromatography followed by an ion-exchange chromatography procedure. Sepharose 124-133 interleukin 4 induced 1 Homo sapiens 96-99 18057708-5 2007 Human erythrocyte isoenzymes, HCA-I and HCA-II, were purified by Sepharose-4B affinity chromatography column with a yield of 66.95 and 62.82%, a specific activity of 3892.3 and 11663.2 EU/mg proteins with 745.1 and 2232.6-fold purification of each isoenzyme, respectively. Sepharose 65-74 cytochrome P450 family 24 subfamily A member 1 Homo sapiens 30-46 17634960-3 2007 In this work, recombinant human granulocyte colony-stimulating factor (rhG-CSF) expressed in Escherichia coli was renatured with simultaneous purification by ion exchange chromatography (IEC) with a Q Sepharose FF column. Sepharose 201-210 colony stimulating factor 3 Homo sapiens 32-69 17924969-8 2007 Sepharose 6MB macrobead (250-350 microm) columns proved to be most effective, selectively removing up to 98% of CD4+ T cells from whole blood. Sepharose 0-9 CD4 molecule Homo sapiens 112-115 17982629-9 2007 The effect of 14-3-3sigma over-expression on tumor cell migration was investigated using an agarose-cell droplet migration assay. Sepharose 92-99 stratifin Homo sapiens 14-25 17933553-8 2007 A protocol was developed to purify recombinant endostatin in the fermentation supernatant to a yield of 24mg/L and 98% purity by the use of SP Sepharose FF cation exchange chromatography, Sepharose-heparin Hi Trap affinity chromatography and gel filtration chromatography. Sepharose 143-152 collagen type XVIII alpha 1 chain Homo sapiens 47-57 17933553-8 2007 A protocol was developed to purify recombinant endostatin in the fermentation supernatant to a yield of 24mg/L and 98% purity by the use of SP Sepharose FF cation exchange chromatography, Sepharose-heparin Hi Trap affinity chromatography and gel filtration chromatography. Sepharose 188-197 collagen type XVIII alpha 1 chain Homo sapiens 47-57 18232482-4 2007 When treated with intracellular hyperthermia, the SPC-A1 cells manifested typical morphological characters of apoptosis by TEM observation, and the SPC-A1 cell DNA was degraded into large fragments by agarose gel electrophoresis assay. Sepharose 201-208 proline rich protein gene cluster Homo sapiens 148-151 17707670-1 2007 Trypsin was purified from the pyloric caeca of bluefish (Pomatomus saltatrix) by ammonium sulfate precipitation, acetone precipitation and soybean trypsin inhibitor-Sepharose 4B affinity chromatography. Sepharose 165-174 kunitz trypsin protease inhibitor Glycine max 147-164 18284054-4 2007 Recombinant PSA was expressed and purified by hydrophobic interaction phenyl Sepharose column and activated by trypsin digestion. Sepharose 77-86 kallikrein related peptidase 3 Homo sapiens 12-15 17823128-8 2007 Using two-dimensional agarose gel electrophoresis we also show that UvsW acts on T4-generated replication intermediates, including Holliday junction-containing X-shaped intermediates and replication fork-shaped intermediates. Sepharose 22-29 UvsW helicase Escherichia phage T4 68-72 18006317-7 2007 Two-dimensional agarose gel electrophoresis revealed that ORC2 depletion increased telomere circle formation, comparable to the overexpression of TRF2DeltaB. Sepharose 16-23 origin recognition complex subunit 2 Homo sapiens 58-62 17609857-3 2007 Refolded fusion thioredoxin/TRAIL was cleaved by enteropeptidase and TRAIL was separated from thioredoxin on Ni-NTA agarose. Sepharose 116-123 TNF superfamily member 10 Homo sapiens 69-74 17988583-3 2007 The recombinant chymase was expressed in E.coli with L-Arabinose induction and purified by Ni-NTA agarose column. Sepharose 98-105 LOW QUALITY PROTEIN: chymase Oryctolagus cuniculus 16-23 17973995-3 2007 RESULTS: CYR61 and WISP3 were purified as fusion proteins with a C-terminal Fc-tag from baculovirus infected SF21 cells using protein G sepharose columns. Sepharose 136-145 cellular communication network factor 1 Homo sapiens 9-14 17973995-3 2007 RESULTS: CYR61 and WISP3 were purified as fusion proteins with a C-terminal Fc-tag from baculovirus infected SF21 cells using protein G sepharose columns. Sepharose 136-145 cellular communication network factor 6 Homo sapiens 19-24 19075825-1 2008 To elucidate whether eukaryotic elongation factor 1A (eEF-1A) in a human hepidermoid cancer cell line (H1355) belonged to the family of the Ni-interacting protein, we analyzed the sequence of peptides obtained by on-Ni-NTA-agarose tryptic digestion of proteins from H1355 cell extract. Sepharose 223-230 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 21-52 19075825-1 2008 To elucidate whether eukaryotic elongation factor 1A (eEF-1A) in a human hepidermoid cancer cell line (H1355) belonged to the family of the Ni-interacting protein, we analyzed the sequence of peptides obtained by on-Ni-NTA-agarose tryptic digestion of proteins from H1355 cell extract. Sepharose 223-230 eukaryotic translation elongation factor 1 alpha 1 Homo sapiens 54-60 17660222-6 2007 PTPN22 620W was detected by SSP-PCR analysis and agarose gel electrophoresis. Sepharose 49-56 protein tyrosine phosphatase non-receptor type 22 Homo sapiens 0-6 17916391-6 2007 This DNA-binding protein was sequentially purified using DEAE-Sephacel, heparin-Sepharose, DNA Affinity, and gel filtration chromatography. Sepharose 80-89 zinc finger protein 763 Homo sapiens 5-24 17761538-4 2007 We showed that on the agarose surface under physiological conditions, E-cadherin produced a weak dimer that immediately dissociated after the depletion of calcium ions. Sepharose 22-29 cadherin 1 Homo sapiens 70-80 17955473-4 2007 We generated p16(ink4a) columns by coupling the protein to activated Sepharose 4B. Sepharose 69-78 cyclin dependent kinase inhibitor 2A Homo sapiens 13-16 17955473-4 2007 We generated p16(ink4a) columns by coupling the protein to activated Sepharose 4B. Sepharose 69-78 cyclin dependent kinase inhibitor 2A Homo sapiens 17-22 17609857-3 2007 Refolded fusion thioredoxin/TRAIL was cleaved by enteropeptidase and TRAIL was separated from thioredoxin on Ni-NTA agarose. Sepharose 116-123 thioredoxin Homo sapiens 94-105 17621593-7 2007 In competitive binding studies, hITLN-1 was eluted from galactose-Sepharose by 100 mM 2-deoxygalactose, a galactofuranosyl disaccharide, d-xylose, and both d- and l-ribose. Sepharose 66-75 intelectin 1 Homo sapiens 32-39 17621593-9 2007 When the N- and C-terminal regions of hITLN-1 were replaced, respectively, with those of mITLN-1, galactose-Sepharose binding was associated with the C-terminal regions. Sepharose 108-117 intelectin 1 Homo sapiens 38-45 17621593-9 2007 When the N- and C-terminal regions of hITLN-1 were replaced, respectively, with those of mITLN-1, galactose-Sepharose binding was associated with the C-terminal regions. Sepharose 108-117 intelectin 1 (galactofuranose binding) Mus musculus 89-96 17621593-10 2007 Finally, hITLN-1 binding to galactose-Sepharose was not affected by the substitution of the Cys residues in the N-terminal region that are necessary for oligomer formation, nor was it affected by the removal of the N-linked oligosaccharide at Asn-163. Sepharose 38-47 intelectin 1 Homo sapiens 9-16 18236782-9 2007 The expression of aldehyde dehydrogenase 3a1 (ALDH3a1), which may be be responsible for DSF-DDC conversion, was detected using RT-PCR and agarose gels electrophoresis. Sepharose 138-145 aldehyde dehydrogenase 3 family member A1 Homo sapiens 18-44 18236782-9 2007 The expression of aldehyde dehydrogenase 3a1 (ALDH3a1), which may be be responsible for DSF-DDC conversion, was detected using RT-PCR and agarose gels electrophoresis. Sepharose 138-145 aldehyde dehydrogenase 3 family member A1 Homo sapiens 46-53 17888672-1 2007 A novel trypsin-papain inhibitor, named PdKI-2, was purified from the seeds of Pithecelobium dumosum seeds by TCA precipitation, Trypsin-Sepharose chromatography and reversed-phase HPLC. Sepharose 137-146 trypsin Ceratitis capitata 8-15 17239614-1 2007 A natural lectin from the serum of the shrimp Litopenaeus vannamei was purified to homogeneity by a single-step affinity chromatography using fetuin-coupled agarose. Sepharose 157-164 ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Homo sapiens 0-1 17853411-1 2007 Type 1 (Th1) granulomas can be studied in mice sensitized with mycobacterium antigens followed by challenge of agarose beads covalently coupled to purified protein derivative. Sepharose 111-118 negative elongation factor complex member C/D, Th1l Mus musculus 8-11 17644519-6 2007 In studies of glycosaminoglycan binding, MIP-1beta-A10C binds to a heparin-Sepharose column as tightly as the wild type protein and more tightly than monomeric variants. Sepharose 75-84 C-C motif chemokine ligand 4 Homo sapiens 41-50 17719580-5 2007 Furthermore, the MnSOD-overexpressing stable transfectants exhibited reduced soft-agarose colony-forming ability and metastatic properties, unlike control cell lines. Sepharose 82-89 superoxide dismutase 2 Homo sapiens 17-22 17927852-10 2007 After blocking PLC-gamma1 signaling pathway, the G1 phase proportion of LoVo cells was increased while the S phase proportion was decreasedu no apoptosis-specific cell shrinkage was found under a light microscope, and no apoptosis-specific DNA ladder was found by agarose gel electrophoresisu no activated Caspase-3 was detected by Western blot, while increased expression of HSP70 was detected. Sepharose 264-271 phospholipase C gamma 1 Homo sapiens 15-25 17763444-7 2007 RESULTS: Multiple isoforms, including possible zymogens, of ADAMTS-4 and ADAMTS-5 were sequestered within the extracellular matrix formed by 3-week chondrocyte-agarose cultures. Sepharose 160-167 ADAM metallopeptidase with thrombospondin type 1 motif 4 Homo sapiens 60-68 17763444-7 2007 RESULTS: Multiple isoforms, including possible zymogens, of ADAMTS-4 and ADAMTS-5 were sequestered within the extracellular matrix formed by 3-week chondrocyte-agarose cultures. Sepharose 160-167 ADAM metallopeptidase with thrombospondin type 1 motif 5 Homo sapiens 73-81 17763444-10 2007 CONCLUSION: In porcine chondrocyte-agarose cultures, a 37-kd ADAMTS-4 isoform appears to be the major matrix protease responsible for the IGD-aggrecanase activity detected in response to exposure to IL-1. Sepharose 35-42 ADAM metallopeptidase with thrombospondin type 1 motif 4 Homo sapiens 61-69 17600626-2 2007 In this report we identified proteins bearing beta1-6 GlcNAc branched N-glycans in three human melanoma cell lines: WM35--from the primary tumour site, as well as WM239 and WM9 from different metastatic sites: the skin and the lymph node, respectively, by tandem mass spectrometry (MS/MS) on PHA-L agarose bound material, followed by immunochemical identification. Sepharose 298-305 UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2 Homo sapiens 46-53 17827718-2 2007 In this study, PAF-AH in early colostrum was purified by ammonium sulfate precipitation, and sequential use of butyl-Toyopearl 650M, DEAE-Sepharose, heparin-Sepharose, hydroxyapatite, chelating-Sepharose and Mono Q HPLC column chromatography. Sepharose 138-147 phospholipase A2 group VII Homo sapiens 15-21 17827718-2 2007 In this study, PAF-AH in early colostrum was purified by ammonium sulfate precipitation, and sequential use of butyl-Toyopearl 650M, DEAE-Sepharose, heparin-Sepharose, hydroxyapatite, chelating-Sepharose and Mono Q HPLC column chromatography. Sepharose 157-166 phospholipase A2 group VII Homo sapiens 15-21 17534582-2 2007 The two extracellular portions of hIL-6R were expressed as soluble fusion proteins with thioredoxin in Escherichia coli and purified by using Ni-NTA agarose. Sepharose 149-156 interleukin 6 receptor Homo sapiens 34-40 17712002-3 2007 METHODS: Methyl-binding domain (MBD) protein was produced using a pET6HMBD plasmid with MBD DNA sequence cloned from rat MeCP2 gene and bound to a column of nickel-agarose resin. Sepharose 164-171 methyl CpG binding protein 2 Rattus norvegicus 121-126 17722186-3 2007 An immunosorbent consisting of a recombinant antitestosterone Fab fragment covalently attached to activated Sepharose was prepared. Sepharose 108-117 FA complementation group B Homo sapiens 62-65 17662035-6 2007 To show that AtS1P directly targets AtbZIP17, cleavage was also demonstrated in an in vitro pull-down assay with agarose bead-immobilized AtS1P. Sepharose 113-120 SITE-1 protease Arabidopsis thaliana 13-18 17662035-6 2007 To show that AtS1P directly targets AtbZIP17, cleavage was also demonstrated in an in vitro pull-down assay with agarose bead-immobilized AtS1P. Sepharose 113-120 Basic-leucine zipper (bZIP) transcription factor family protein Arabidopsis thaliana 36-44 17662035-6 2007 To show that AtS1P directly targets AtbZIP17, cleavage was also demonstrated in an in vitro pull-down assay with agarose bead-immobilized AtS1P. Sepharose 113-120 SITE-1 protease Arabidopsis thaliana 138-143 18048959-2 2007 Human erythrocyte CAI and II isozymes were purified by Sepharose-4B L-tyrosine affinity chromatography column with a yield of 30% and 40%, a specific activity of 920 and 8,000 EU/mg protein, respectively. Sepharose 55-64 carbonic anhydrase 1 Homo sapiens 18-28 18051864-10 2007 It was observed that the purified LPTS-L inhibited the activity of telomerase greatly, similarly with that of LPTS-L protein purified by Ni Sepharose 4B. Sepharose 140-149 PIN2 (TERF1) interacting telomerase inhibitor 1 Homo sapiens 34-38 18051864-10 2007 It was observed that the purified LPTS-L inhibited the activity of telomerase greatly, similarly with that of LPTS-L protein purified by Ni Sepharose 4B. Sepharose 140-149 PIN2 (TERF1) interacting telomerase inhibitor 1 Homo sapiens 110-114 18051864-11 2007 Our results suggest that phosphocellulose P11 plus Sephadex G-100 chromatography could substitute for Ni Sepharose 4B affinity chromatography for preparation of purified LPTS-L protein. Sepharose 105-117 PIN2 (TERF1) interacting telomerase inhibitor 1 Homo sapiens 170-174 17598176-5 2007 Dystroglycan, a non-integrin Schwann cell receptor for laminin-2 identified previously, was also found to bind to laminin-2-Sepharose. Sepharose 124-133 dystroglycan 1 Homo sapiens 0-12 17675514-2 2007 Recombinant histidine-tagged FPR (rHis-FPR) was purified in lysophosphatidyl glycerol (LPG) by Ni(2+)-NTA agarose chromatography to >95% purity with high yield. Sepharose 106-113 formyl peptide receptor 1 Homo sapiens 29-32 17574211-3 2007 STD NMR experiments of the protein-enriched Sepharose matrix in the presence of a binding ligand (cytidine 5"-triphosphate, CTP) and a non-binding ligand (alpha/beta-glucose) clearly show that CTP binds to the immobilised enzyme, whereas glucose has no affinity. Sepharose 44-53 solute carrier family 25 member 1 Homo sapiens 193-196 17609857-3 2007 Refolded fusion thioredoxin/TRAIL was cleaved by enteropeptidase and TRAIL was separated from thioredoxin on Ni-NTA agarose. Sepharose 116-123 thioredoxin Homo sapiens 16-27 17609857-3 2007 Refolded fusion thioredoxin/TRAIL was cleaved by enteropeptidase and TRAIL was separated from thioredoxin on Ni-NTA agarose. Sepharose 116-123 TNF superfamily member 10 Homo sapiens 28-33 17611673-6 2007 For IL-1 receptor antagonist gene (IL-1RN), penta-allelic variable number of tandem repeats (VNTR) was determined by PCR-standard agarose gel electrophoresis. Sepharose 130-137 interleukin 1 receptor antagonist Homo sapiens 4-28 17611673-6 2007 For IL-1 receptor antagonist gene (IL-1RN), penta-allelic variable number of tandem repeats (VNTR) was determined by PCR-standard agarose gel electrophoresis. Sepharose 130-137 interleukin 1 receptor antagonist Homo sapiens 35-41 17610480-6 2007 Western blot (confirmed by immunohistochemical analysis) after agarose gel electrophoresis showed the presence of MUC2, MUC5AC and MUC5B in the mucus. Sepharose 63-70 mucin 2, oligomeric mucus/gel-forming Homo sapiens 114-118 17610480-6 2007 Western blot (confirmed by immunohistochemical analysis) after agarose gel electrophoresis showed the presence of MUC2, MUC5AC and MUC5B in the mucus. Sepharose 63-70 mucin 5B, oligomeric mucus/gel-forming Homo sapiens 131-136 17618558-10 2007 The fusion protein SRG4 with 6xHis tag was confirmed by Western blot and was purified by Ni-NTA Agarose. Sepharose 96-103 Sad1 and UNC84 domain containing 5 Mus musculus 19-23 17708817-2 2007 The phosphorothiate VEGF ODN was transferred into HL-60 cells in vitro by using cation poly mediated method, the inhibitory rate of cell proliferation was assayed by MTT, expression of VEGF mRNA was measured by RT-PCR, cell apoptosis was detected by cell morphology observation, DNA agarose gel electrophoresis and flow cytometer (FCM). Sepharose 283-290 vascular endothelial growth factor A Homo sapiens 20-24 17669278-4 2007 Surprisingly, the Rat FDH molecule with two typical liver proteins, carbamoyl-phosphate synthetase 1 (CPS1) and betaine homocysteine S-methyltransferase (BHMT) were co-purified to homogeneity on SBTI-coupled Sepharose and Sephacryl S-200 followed by Superdex 200 FPLC columns. Sepharose 208-217 aldehyde dehydrogenase 1 family, member L1 Rattus norvegicus 22-25 17493687-3 2007 In these studies, we compared carbohydrate-dependent binding of mouse plasma MBL-A and MBL-C to mannan-sepharose beads and to intact bacteria isolated as pathogens from mice. Sepharose 103-112 mannose-binding lectin (protein A) 1 Mus musculus 77-82 17493687-3 2007 In these studies, we compared carbohydrate-dependent binding of mouse plasma MBL-A and MBL-C to mannan-sepharose beads and to intact bacteria isolated as pathogens from mice. Sepharose 103-112 mannose-binding lectin (protein C) 2 Mus musculus 87-92 17493687-7 2007 By comparison, immunoreactive MBL-A (a ladder of approximately 175kDa and larger bands) was identified in these GlcNAc eluates from mannan-sepharose beads, S. aureus and K. oxytoca but not P. aeruginosa. Sepharose 139-148 mannose-binding lectin (protein A) 1 Mus musculus 30-35 17541568-0 2007 Analysis of mismatched DNA by mismatch binding ligand (MBL)-Sepharose affinity chromatography. Sepharose 60-69 mannose binding lectin 2 Homo sapiens 30-53 17929568-1 2007 A trypsin inhibitor, isolated from whole-wheat grain (Triticum aestivum L.) by the method of bio-specific chromatography on trypsin-Sepharose, was potent in inhibiting human salivary alpha-amylase. Sepharose 132-141 amylase alpha 1A Homo sapiens 174-196 17541568-0 2007 Analysis of mismatched DNA by mismatch binding ligand (MBL)-Sepharose affinity chromatography. Sepharose 60-69 mannose binding lectin 2 Homo sapiens 55-58 17541568-3 2007 These three MBL-Sepharose columns showed characteristic elution profiles for DNA duplexes containing mismatched base pairs. Sepharose 16-25 mannose binding lectin 2 Homo sapiens 12-15 17541568-9 2007 Figure MBL-Sepharose affinity chromatography successfully separates the mismatched duplex DNA from fully matched duplex. Sepharose 11-20 mannose binding lectin 2 Homo sapiens 7-10 17565155-8 2007 Binding of deguelin to Hsp90 was examined by docking analysis and by competition binding experiments with ATP-Sepharose beads. Sepharose 110-119 heat shock protein, 3 Mus musculus 23-28 17464559-3 2007 CA I isozyme was measured by radial immunodiffusion using monoclonal antibody (CA I) in agarose plates. Sepharose 88-95 carbonic anhydrase 1 Homo sapiens 0-4 17464559-3 2007 CA I isozyme was measured by radial immunodiffusion using monoclonal antibody (CA I) in agarose plates. Sepharose 88-95 carbonic anhydrase 1 Homo sapiens 79-83 17368531-4 2007 Elastin tubes were filled with agarose gel containing basic fibroblast growth factor (bFGF) to allow sustained release of growth factor. Sepharose 31-38 elastin Rattus norvegicus 0-7 17368531-4 2007 Elastin tubes were filled with agarose gel containing basic fibroblast growth factor (bFGF) to allow sustained release of growth factor. Sepharose 31-38 fibroblast growth factor 2 Rattus norvegicus 86-90 17581677-8 2007 MUC-1 amplicons were obtained by PCR and observed on agarose and polyacrylamide gel after electrophoresis. Sepharose 53-60 mucin 1, cell surface associated Homo sapiens 0-5 17416474-7 2007 We then attached BDNF-secreting cells to the cochlear implant electrode via an agarose gel, and implanted the electrode in the scala tympani. Sepharose 79-86 brain derived neurotrophic factor Homo sapiens 17-21 17584649-5 2007 Glutathione-Sepharose beads were used to purify GST- PAK6-N fusion protein. Sepharose 12-21 p21 (RAC1) activated kinase 6 Homo sapiens 53-57 17657985-2 2007 AIM: We evaluated the serum apo B-48 concentrations by chemiluminescence enzyme immunoassay (CLEIA), which was recently introduced as Lumipulse f fully automated immunosaasy analyzer by Fujirebio Inc (Tokyo, Japan), and performed immunoblotting on agarose gel electrophoresis with anti-apo B-48 antibody. Sepharose 248-255 apolipoprotein B Homo sapiens 28-36 17713275-4 2007 Then the recombinant human cytochrome C was purified to 80% homogeneity after two times cation exchange chromatography on SP-Sepharose Fast Flow. Sepharose 125-134 cytochrome c, somatic Homo sapiens 27-39 17657985-7 2007 Anti-apo B-48 antibody was reactive over a wide range from origin to the position of very-low-density lipoproteins in immunoblotting after agarose gel electrophoresis. Sepharose 139-146 apolipoprotein B Homo sapiens 5-13 17505205-6 2007 The coding exon of the GJB2 gene was polymerase chain reaction amplified, followed by restriction enzyme digestion with ApaI and analysis by agarose gel. Sepharose 141-148 gap junction protein beta 2 Homo sapiens 23-27 17160018-6 2007 Suppression of PBK expression using small interfering RNA did not prevent progression through the cell cycle, but caused decreased proliferation over time in culture, and reduced clonogenic growth in soft agarose. Sepharose 205-212 PDZ binding kinase Homo sapiens 15-18 17399701-12 2007 Immunoblots of tears using agarose gel electrophoresis and chemiluminescence detection provide a semi-quantitative assay for mucin protein that will be useful for comparisons with tears from diseased eyes or after pharmacological intervention. Sepharose 27-34 LOC100508689 Homo sapiens 125-130 17262722-2 2007 CRP was isolated from porcine acute-phase serum by affinity chromatography on agarose, coupled with phosphorylethanolamine and polyclonal antibodies to porcine CRP were purified from antiserum raised in sheep immunized with porcine CRP. Sepharose 78-85 C-reactive protein Ovis aries 0-3 17322299-11 2007 Moreover, pro-MMP-13 is also activated by a secreted factor, which is absorbed by gelatin-Sepharose and reconstituted with MMP-9. Sepharose 90-99 matrix metallopeptidase 13 Homo sapiens 14-20 17022065-5 2007 Moreover, Western blot analysis revealed selective adsorption of vitronectin onto HA/agarose. Sepharose 85-92 vitronectin Homo sapiens 65-76 17185469-3 2007 Herein, we characterize the neutrophil response in G-CSFR(-/-) mice following intratracheal injection with Pseudomonas aeruginosa-laden agarose beads, modeling the pulmonary infection observed in many patients with cystic fibrosis. Sepharose 136-143 colony stimulating factor 3 receptor (granulocyte) Mus musculus 51-57 17210183-2 2007 Blue-Sepharose is often used to remove bovine serum albumin (BSA) from samples, but when we applied BSA to Blue-Sepharose in 20 mM phosphate, pH 7.0, 50%-60% of the protein flowed through the column; however, complete binding of BSA was achieved by the addition of 2 M ammonium sulfate (AS) to the column equilibration buffer and the sample. Sepharose 5-14 albumin Homo sapiens 46-59 17197255-6 2007 Current protocols for the purification of both native and polyHis-tagged or glutathione-S-transferase (GST)-tagged EF-Tu proteins and their variants using conventional procedures and the Ni-NTA-Agarose or Glutathione Sepharose are presented. Sepharose 217-226 Tu translation elongation factor, mitochondrial Homo sapiens 115-120 17511605-5 2007 Interaction of LF and MPO with CP-Sepharose is blocked at ionic strength above 0.3 M NaCl and at pH below 4.1 (LF) and 3.9 (MPO). Sepharose 34-43 myeloperoxidase Homo sapiens 22-25 17511605-5 2007 Interaction of LF and MPO with CP-Sepharose is blocked at ionic strength above 0.3 M NaCl and at pH below 4.1 (LF) and 3.9 (MPO). Sepharose 34-43 ceruloplasmin Homo sapiens 31-33 17511605-5 2007 Interaction of LF and MPO with CP-Sepharose is blocked at ionic strength above 0.3 M NaCl and at pH below 4.1 (LF) and 3.9 (MPO). Sepharose 34-43 myeloperoxidase Homo sapiens 124-127 17625759-5 2007 A portion of the CCR5 gene was amplified by PCR from genomic DNA followed by agarose gel electrophoresis. Sepharose 77-84 C-C motif chemokine receptor 5 Homo sapiens 17-21 17257670-6 2007 However, in agarose, cells exposed to FGF-2 during expansion produced less sGAG within TGF-beta1-supplemented groups over those cultures treated with TGF-beta1 alone. Sepharose 12-19 fibroblast growth factor 2 Rattus norvegicus 38-43 17287021-8 2007 Coating of agarose beads with recombinant DL2 and DL3 enhanced their encapsulation and melanization by Drosophila hemocytes in vitro. Sepharose 11-18 lectin-37Da Drosophila melanogaster 42-45 17063480-6 2007 Agarose gel electrophoresis showed the presence of low-molecular-weight deoxyribonucleic acid (DNA) fragments of adherent wild-type cells cultured with TNF-alpha (1.0 ng/ml) or TGF-beta1 (5.0 ng/ml). Sepharose 0-7 tumor necrosis factor Rattus norvegicus 152-161 17063480-6 2007 Agarose gel electrophoresis showed the presence of low-molecular-weight deoxyribonucleic acid (DNA) fragments of adherent wild-type cells cultured with TNF-alpha (1.0 ng/ml) or TGF-beta1 (5.0 ng/ml). Sepharose 0-7 transforming growth factor, beta 1 Rattus norvegicus 177-186 17600883-3 2007 MATERIALS AND METHODS: CEA was isolated by immunoaffinity chromatography on an anti-CEA IgG-Sepharose 4B column. Sepharose 92-101 CEA cell adhesion molecule 3 Homo sapiens 23-26 17287021-8 2007 Coating of agarose beads with recombinant DL2 and DL3 enhanced their encapsulation and melanization by Drosophila hemocytes in vitro. Sepharose 11-18 lectin-37Db Drosophila melanogaster 50-53 17249711-10 2007 The enrichment of IFNalpha3 and EDN was particularly effective by batch adsorption using SP Sepharose 4 Fast Flow beads equilibrated with acetic acid (pH 6.0). Sepharose 92-101 ribonuclease A family member 2 Homo sapiens 32-35 16934939-1 2007 A lectin from the hemolymph of the banana shrimp Fenneropenaeus merguiensis was purified by affinity chromatography on a fetuin-agarose column following by gel filtration on a Superose-12 column. Sepharose 128-135 lectin Musa acuminata 2-8 17096387-5 2007 However at pH 4 on SP Sepharose XL, Fab dynamic binding capacity increased from 130 to 160 (mg/mL media) as mobile phase conductivity changed from 1 to 5 mS/cm. Sepharose 22-31 FA complementation group B Homo sapiens 36-39 17092579-8 2007 Using soybean trypsin inhibitor (SBTI)-sepharose affinity column, we have partially purified the LISPDE protein, which has an estimated molecular mass of 33 kDa and selectively degrades native Sp1 protein. Sepharose 39-48 kunitz trypsin protease inhibitor Glycine max 14-31 17156102-1 2007 A detailed investigation of the binding of secretory component to immunoglobulin A (IgA) in human secretory IgA2 (S-IgA2) was made possible by the development of a new method of purifying S-IgA1, S-IgA2 and free secretory component from human colostrum using thiophilic gel chromatography and chromatography on Jacalin-agarose. Sepharose 319-326 immunoglobulin heavy variable 4-38-2-like Homo sapiens 66-88 17285538-3 2007 The genotypes of NOS3 gene VNTR polymorphism were determined by polymerase chain reaction and agarose gel electrophoresis. Sepharose 94-101 nitric oxide synthase 3 Homo sapiens 17-21 17150184-7 2007 We demonstrate that the transfected cells secrete substantial amounts of glycosylated ecSOD, detected by Western blot analyses, ConA-Sepharose affinity chromatography and activity measurements. Sepharose 133-142 superoxide dismutase 3, extracellular Mus musculus 86-91 17311871-3 2007 Biologically active pure human Epo-bp (MW=29 kDa) was then purified by Epo-agarose chromatography after cleaving off the GST by thrombin. Sepharose 75-82 erythropoietin Homo sapiens 31-34 17103164-11 2007 The recombinant 1-Cys Prx was purified by Ni(2+)-nitrilotriacetic acid (Sepharose). Sepharose 72-81 periaxin Homo sapiens 22-25 17161030-3 2007 We have recently proposed from studies using yeast cells lacking the gene encoding Hsp12p (Deltahsp12 yeast) and from incorporation of Hsp12p into agarose, used as a model system for the beta-glucan layer of the cell wall, that the hydrophilic stress response cell wall protein Hsp12p acts as a cell wall plasticizer. Sepharose 147-154 lipid-binding protein HSP12 Saccharomyces cerevisiae S288C 135-141 17161030-3 2007 We have recently proposed from studies using yeast cells lacking the gene encoding Hsp12p (Deltahsp12 yeast) and from incorporation of Hsp12p into agarose, used as a model system for the beta-glucan layer of the cell wall, that the hydrophilic stress response cell wall protein Hsp12p acts as a cell wall plasticizer. Sepharose 147-154 lipid-binding protein HSP12 Saccharomyces cerevisiae S288C 135-141 17136731-1 2007 We have studied the equilibrium uptake behavior and mass transfer rate of recombinant apolipoprotein A-I(Milano) (apo A-I(M)) on Q Sepharose HP under non-denaturing, partially denaturing, and fully denaturing conditions. Sepharose 131-140 apolipoprotein A1 Homo sapiens 86-112 17136731-1 2007 We have studied the equilibrium uptake behavior and mass transfer rate of recombinant apolipoprotein A-I(Milano) (apo A-I(M)) on Q Sepharose HP under non-denaturing, partially denaturing, and fully denaturing conditions. Sepharose 131-140 apolipoprotein A1 Homo sapiens 114-121 17136731-7 2007 The rate of apo A-I(M) mass transfer on Q Sepharose HP was characterized using a macropore diffusion model. Sepharose 42-51 apolipoprotein A1 Homo sapiens 12-19 17164493-8 2007 VWD type 2M is featured by the presence of all VWF multimers in a low resolution agarose gel, normal or slightly prolonged BT, decreased RIPA, a poor response of VWF:RCo and a good response of FVIII and VWF:CB to DDAVP and therefore clearly in between dominant type 1 and 2U. Sepharose 81-88 von Willebrand factor Homo sapiens 47-50 17311507-2 2007 METHODS: Serum electrophoresis was carried out on a cellulose acetate support up until 1997, and then using capillary zone electrophoresis systems, with M-protein identification carried out by agarose gel immunofixation. Sepharose 193-200 myomesin 2 Homo sapiens 153-162 17464768-6 2007 In particular, IFN-gamma-like activity gave rise to an enhancement of Ca phagocytosis by MO, whereas MIF inhibited MO migration in agarose. Sepharose 131-138 macrophage migration inhibitory factor Oncorhynchus mykiss 101-104 17005160-7 2006 Activated K-ras protein from lung was affinity precipitated with a Raf-1 ras binding domain-glutathione-S-transferase fusion protein bound to glutathione-agarose beads, followed by Western blotting, K-ras antibody treatment, and chemiluminescent detection. Sepharose 154-161 Kirsten rat sarcoma viral oncogene homolog Mus musculus 10-15 17506480-0 2007 High-molecular intestinal alkaline phosphatase by agarose gel electrophoresis. Sepharose 50-57 alkaline phosphatase, intestinal Homo sapiens 15-46 17884915-4 2007 Two-dimensional neutral agarose gel electrophoresis (2DNAGE) and 5" end mapping by ligation-mediated PCR (LM-PCR) identified the region of the canonical mTERF-binding site as a replication pause site. Sepharose 24-31 tripartite motif-containing 17 Mus musculus 153-158 17180450-3 2007 Cross-reactivity between anti-human haptoglobin antiserum and canine haptoglobin was found when agarose gel immunodiffusion and ELISA tests were performed. Sepharose 96-103 haptoglobin Canis lupus familiaris 36-47 17180450-3 2007 Cross-reactivity between anti-human haptoglobin antiserum and canine haptoglobin was found when agarose gel immunodiffusion and ELISA tests were performed. Sepharose 96-103 haptoglobin Canis lupus familiaris 69-80 17181147-4 2006 Using the strong affinity of avidin for biotin, we have isolated affinity-labeled caspase 6 from apoptotic cytosolic extracts of cells overexpressing procaspase 6 by treatment with 12c, which contains biotin attached to the N(epsilon)-lysine of the inhibitor by a 22.5 A linker arm, followed by affinity purification on monomeric avidin-sepharose beads. Sepharose 337-346 caspase 6 Homo sapiens 82-91 17005160-7 2006 Activated K-ras protein from lung was affinity precipitated with a Raf-1 ras binding domain-glutathione-S-transferase fusion protein bound to glutathione-agarose beads, followed by Western blotting, K-ras antibody treatment, and chemiluminescent detection. Sepharose 154-161 v-raf-leukemia viral oncogene 1 Mus musculus 67-72 16917940-6 2006 VNTR polymorphisms of the DRD4 gene were determined by standard PCR followed by agarose gel electrophoresis. Sepharose 80-87 dopamine receptor D4 Homo sapiens 26-30 17001657-6 2006 The results showed that the implantation of agarose beads in mucosa induced the recruitment of inflammatory CD45 positive cells. Sepharose 44-51 protein tyrosine phosphatase, receptor type, C Mus musculus 108-112 17223789-3 2006 To prevent the influence of nonspecific association of non-native proteins on their interaction with GroEL and make easier the recording of the complexing, the proteins were covalently attached to BrCN-activated Sepharose. Sepharose 212-221 GroEL Escherichia coli 101-106 16788146-3 2006 In the present study, histidine-tagged hRS1 was expressed in oocytes or Sf9 cells and purified using nickel(II)-charged nitrilotriacetic acid-agarose. Sepharose 142-149 retinoschisin 1 Homo sapiens 39-43 17142967-1 2006 A recombinant rat aminopeptidase-B (Ap-B) was expressed as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 harboring a plasmid pGEX-Ap-B and was purified by glutathione-Sepharose 4B and Q-Sepharose columns. Sepharose 195-204 arginyl aminopeptidase Rattus norvegicus 18-34 17142967-1 2006 A recombinant rat aminopeptidase-B (Ap-B) was expressed as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 harboring a plasmid pGEX-Ap-B and was purified by glutathione-Sepharose 4B and Q-Sepharose columns. Sepharose 195-204 arginyl aminopeptidase Rattus norvegicus 36-40 17142967-1 2006 A recombinant rat aminopeptidase-B (Ap-B) was expressed as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 harboring a plasmid pGEX-Ap-B and was purified by glutathione-Sepharose 4B and Q-Sepharose columns. Sepharose 214-223 arginyl aminopeptidase Rattus norvegicus 18-34 17142967-1 2006 A recombinant rat aminopeptidase-B (Ap-B) was expressed as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 harboring a plasmid pGEX-Ap-B and was purified by glutathione-Sepharose 4B and Q-Sepharose columns. Sepharose 214-223 arginyl aminopeptidase Rattus norvegicus 36-40 17257501-3 2006 Cytotoxicity exerted by FasL/anti-DR5 mAb on tumor cell lines was measured by MTT assay and the induced apoptosis was determined by agarose gel electrophoresis. Sepharose 132-139 Fas ligand Homo sapiens 24-28 16904905-2 2006 The binding of P. aeruginosa SecA (PaSecA) to the SP-Sepharose column was facilitated by ammonium sulfate fractionation but was not necessary for E. coli SecA (EcSecA) as the later bound more efficiently. Sepharose 53-62 preprotein translocase subunit SecA Pseudomonas aeruginosa PAO1 29-33 17164749-7 2006 CONCLUSIONS: T4, T3, and T4-agarose are pro-angiogenic in the three-dimensional human microvascular endothelial sprouting model, an action that is initiated at the plasma membrane, involves avb3 integrin receptors, and is MAPK-dependent. Sepharose 28-35 mitogen-activated protein kinase 3 Homo sapiens 222-226 16904905-2 2006 The binding of P. aeruginosa SecA (PaSecA) to the SP-Sepharose column was facilitated by ammonium sulfate fractionation but was not necessary for E. coli SecA (EcSecA) as the later bound more efficiently. Sepharose 53-62 preprotein translocase subunit SecA Pseudomonas aeruginosa PAO1 37-41 16934821-2 2006 Polymerase chain reaction (PCR), lambda exonuclease digestion combined with (AC)(5)-Sepharose DNA affinity chromatography were used to produce c-jun promoter with a (GT)(5) tail at each 3" end. Sepharose 84-93 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 143-148 16934821-4 2006 In solution, tailed c-jun promoter (60 nM) and competitor poly dI:dC (30 ng/microl) was incubated with crude HEK293 nuclear extract to form a large protein-promoter complex, and the complex was then trapped by (AC)(5)-Sepharose by centrifugation or on a column. Sepharose 218-227 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 20-25 17096525-2 2006 HAp was formed using aqueous solutions of calcium chloride and disodium hydrophosphate, which were set in a conventional agarose electrophoresis apparatus. Sepharose 121-128 reticulon 3 Homo sapiens 0-3 17096525-1 2006 As a first step toward hydroxyapatite (HAp) formation in agarose hydrogels, we have tailored the internal chemistry using an electrophoresis approach. Sepharose 57-64 reticulon 3 Homo sapiens 39-42 16822680-4 2006 In line with these, LPS (1 microgml(-1))- and TNF-alpha (200 ngml(-1))-induced DNA fragmentation, assessed by agarose gel electrophoresis, was significantly reduced by treatment with cilostazol (10 microM) as well as by dibutyryl cAMP (100 microM). Sepharose 110-117 tumor necrosis factor Homo sapiens 46-55 17131818-0 2006 [Calcium-dependent interaction of transducin with calmodulin-sepharose]. Sepharose 61-70 calmodulin Bos taurus 50-60 17131818-1 2006 It has been shown by affinity chromatography on calmodulin-sepharose that transducin, a G protein of bovine retinal rod outer segments interacts with Ca(2+)-calmodulin. Sepharose 59-68 calmodulin Bos taurus 48-58 17131818-1 2006 It has been shown by affinity chromatography on calmodulin-sepharose that transducin, a G protein of bovine retinal rod outer segments interacts with Ca(2+)-calmodulin. Sepharose 59-68 calmodulin Bos taurus 157-167 17131840-1 2006 The titin isoform composition in skeletal and cardiac muscles of humans and animals has been studied using SDS elecrtophoresis in agarose-strengthened 1.3-2.3% polyacrylamide gels modified by us and immunoblot analysis in order to reveal new titin isoforms with molecular weights of more than 3700 kDa. Sepharose 130-137 titin Homo sapiens 4-9 16944460-3 2006 IEF of serum AFP was run in 1% agarose IEF gel with 3% Pharmalyte 4.5-5.4. Sepharose 31-38 alpha fetoprotein Homo sapiens 13-16 16788089-8 2006 Therefore, immobilized KBA (linked to Sepharose beads) selectively precipitated p12-LO from platelet lysates but failed to bind cPLA2. Sepharose 38-47 arachidonate 12-lipoxygenase, 12S type Homo sapiens 80-86 16810680-5 2006 Using protein A-sepharose, the percentage of the plasma CK activity found in immune complexes was determined to be correlated with the CK-MM autoantibody level at lower CK levels (<1,022 U/L). Sepharose 16-25 creatine kinase, M-type Homo sapiens 135-140 17112229-4 2006 In this work glutathione S-transferase (GST), a well-known and abundant BB-target protein, was isolated from liver cytosol of rats treated with 14C-BB by use of a glutathione (GSH)-agarose affinity column and further resolved by reverse-phase high-performance liquid chromatography (HPLC) into subunits M1, M2, A1, A2 and A3. Sepharose 181-188 hematopoietic prostaglandin D synthase Rattus norvegicus 13-38 17112229-4 2006 In this work glutathione S-transferase (GST), a well-known and abundant BB-target protein, was isolated from liver cytosol of rats treated with 14C-BB by use of a glutathione (GSH)-agarose affinity column and further resolved by reverse-phase high-performance liquid chromatography (HPLC) into subunits M1, M2, A1, A2 and A3. Sepharose 181-188 hematopoietic prostaglandin D synthase Rattus norvegicus 40-43 16886225-6 2006 However, agarose-treated DCs showed higher activation of nuclear factor kappaB (NFkappaB) 24 h after the initial stimulation of DCs. Sepharose 9-16 nuclear factor kappa B subunit 1 Homo sapiens 57-78 16886225-6 2006 However, agarose-treated DCs showed higher activation of nuclear factor kappaB (NFkappaB) 24 h after the initial stimulation of DCs. Sepharose 9-16 nuclear factor kappa B subunit 1 Homo sapiens 80-88 17111088-1 2006 A novel lectin, PPL, was isolated from the mantle of penguin wing oyster (Pteria penguin) by affinity chromatography on mucin-Sepharose 4B and cation exchange chromatography on HiTrap SP. Sepharose 126-135 periplakin Oryctolagus cuniculus 16-19 16932880-5 2006 Reactive Red 120-Agarose resin was used efficiently to purify the expressed LMW-GS proteins from bacteria, with the lactic acid-sodium lactate buffer (pH 4.5) which contained low concentration SDS as elution solution. Sepharose 17-24 seed storage protein Triticum aestivum 76-82 17035502-3 2006 Using affinity-chromatography on calmodulin-agarose, we could identify the inner envelope translocon component Tic32 as the predominant calmodulin-binding protein of this membrane. Sepharose 44-51 calmodulin 1 Homo sapiens 33-43 17035502-3 2006 Using affinity-chromatography on calmodulin-agarose, we could identify the inner envelope translocon component Tic32 as the predominant calmodulin-binding protein of this membrane. Sepharose 44-51 calmodulin 1 Homo sapiens 136-146 16790370-2 2006 The EPO-specific immunoaffinity column (IAC) was generated by covalent immobilization of anti-EPO polyclonal antibodies on Sepharose 4B support. Sepharose 123-132 erythropoietin Homo sapiens 4-7 17011980-5 2006 After digestion with MspI, common CCND1 polymorphic alleles were analyzed by means of agarose gel electrophoresis. Sepharose 86-93 cyclin D1 Homo sapiens 34-39 16870265-4 2006 The purified MBP-SLA-2-(G4S)3-beta(2)m protein was cleaved by factor Xa protease, and further purified by DEAE-Sepharose chromatography. Sepharose 111-120 myelin basic protein Sus scrofa 13-16 16870265-4 2006 The purified MBP-SLA-2-(G4S)3-beta(2)m protein was cleaved by factor Xa protease, and further purified by DEAE-Sepharose chromatography. Sepharose 111-120 src-like-adapter 2 Sus scrofa 17-22 17198584-3 2006 Nest-PCR was used to amplify the exons 18, 19, and 21 of the EGFR gene and the PCR products were purified by agarose electrophoresis and then sequenced. Sepharose 109-116 epidermal growth factor receptor Homo sapiens 61-65 16919604-4 2006 In this study, DNA binding activity of SpnA is demonstrated by both agarose gel mobility shift assay and restriction enzyme protection assay. Sepharose 68-75 spindle A Drosophila melanogaster 39-43 16884715-2 2006 Copper complexes of (-)-epicatechin gallate and (-)-epigallocatechin gallate were found to inhibit the enzymatic activity of Ribonuclease A (RNase A) as revealed by an agarose gel based assay and urea denatured gel electrophoresis. Sepharose 168-175 ribonuclease A family member 1, pancreatic Homo sapiens 125-139 16884715-2 2006 Copper complexes of (-)-epicatechin gallate and (-)-epigallocatechin gallate were found to inhibit the enzymatic activity of Ribonuclease A (RNase A) as revealed by an agarose gel based assay and urea denatured gel electrophoresis. Sepharose 168-175 ribonuclease A family member 1, pancreatic Homo sapiens 141-148 17096525-5 2006 The time needed to reach complete HAp formation was 30 min, and 130 ng of HAp was formed in 1 mg of agarose hydrogel when the equilibrium swelling state was reached. Sepharose 100-107 reticulon 3 Homo sapiens 74-77 16865786-5 2006 RESULTS: The product of PCR from plasmid pGEM-T-hALR was examined by 1.5% sepharose electrophoresis. Sepharose 74-83 growth factor, augmenter of liver regeneration Homo sapiens 48-52 16883544-2 2006 METHODS: The genotypes and alleles of repeat sequences of DC-SIGN and DC-SIGNR neck region were typed by PCR, agarose gel electrophoresis and sequencing. Sepharose 110-117 CD209 molecule Homo sapiens 58-65 16883544-2 2006 METHODS: The genotypes and alleles of repeat sequences of DC-SIGN and DC-SIGNR neck region were typed by PCR, agarose gel electrophoresis and sequencing. Sepharose 110-117 C-type lectin domain family 4 member M Homo sapiens 70-78 17081438-10 2006 RESULTS: The size of the RT-PCR product of hIL-1ra fragments was approximately 500 bp in agarose gel electrophoresis. Sepharose 89-96 interleukin 1 alpha Homo sapiens 43-48 16827661-2 2006 Type I antibodies were purified using Sepharose-bound apo-GAPDH that was treated with glutaraldehyde to stabilize the enzyme in the tetrameric form. Sepharose 38-47 glyceraldehyde-3-phosphate dehydrogenase Oryctolagus cuniculus 58-63 16513210-3 2006 Using affinity chromatography, we applied extracts of DU 145 prostate cancer cells over PTHrP (140-173)-Sepharose and eluted with 8 M urea. Sepharose 104-113 parathyroid hormone like hormone Homo sapiens 88-93 16621595-7 2006 The biotinylated CB2-112 fusion protein was cleaved by the tobacco etch virus protease at specifically inserted sites, and deposited onto monomeric avidin agarose beads. Sepharose 155-162 cannabinoid receptor 2 Homo sapiens 17-24 16565420-5 2006 In addition, beta2-microglobulin was copurified with IgG on protein G-Sepharose. Sepharose 70-79 beta-2-microglobulin Sus scrofa 13-32 16817319-8 2006 Analysis of the proteins that bind to an nJacalin-sepharose column revealed the binding of six to eight proteins, and significant among them is a protein at approximately 110 kDa, which appears to be oxygen-regulated protein 150 (ORP150) (endoplasmic reticulum chaperone) as identified by its isoelectric point, two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometric analysis. Sepharose 50-59 hypoxia up-regulated 1 Homo sapiens 200-228 16817319-8 2006 Analysis of the proteins that bind to an nJacalin-sepharose column revealed the binding of six to eight proteins, and significant among them is a protein at approximately 110 kDa, which appears to be oxygen-regulated protein 150 (ORP150) (endoplasmic reticulum chaperone) as identified by its isoelectric point, two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and mass spectrometric analysis. Sepharose 50-59 hypoxia up-regulated 1 Homo sapiens 230-236 16819381-5 2006 AFP electrophoresis using lectin-containing agarose gel identifies L3-AFP by the affinity of its specific carbohydrate chain to lectin. Sepharose 44-51 alpha fetoprotein Homo sapiens 0-3 16819381-5 2006 AFP electrophoresis using lectin-containing agarose gel identifies L3-AFP by the affinity of its specific carbohydrate chain to lectin. Sepharose 44-51 alpha fetoprotein Homo sapiens 70-73 17025179-6 2006 It was shown that affinity chromatography of the product of expression, the chimeric protein GST-LuxR, on a column with glutathione-agarose resulted in its copurification with the proteins GroEL and Lon. Sepharose 132-139 GroEL Escherichia coli 189-194 17025179-6 2006 It was shown that affinity chromatography of the product of expression, the chimeric protein GST-LuxR, on a column with glutathione-agarose resulted in its copurification with the proteins GroEL and Lon. Sepharose 132-139 putative ATP-dependent Lon protease Escherichia coli 199-202 16806017-8 2006 The purity of scFv C1 by nickel-agarose column was above 95% and its yield was about 0.8 mg/L. Sepharose 32-39 immunglobulin heavy chain variable region Homo sapiens 14-18 16806025-2 2006 METHODS: GST-MT-2a fusion protein was expressed after IPTG induction and further purified with Glutathione Sepharose 4B. Sepharose 107-119 metallothionein 2A Homo sapiens 13-18 16773246-0 2006 Identification of a glutathione S-transferase without affinity for glutathione sepharose in human kidney. Sepharose 79-88 glutathione S-transferase kappa 1 Homo sapiens 20-45 16740724-6 2006 Consistent with the apoptosis-inducing activity of T/t-common, we found that T/t-common could specifically inhibit the soft-agarose colony-forming ability of the HER2/neu-overexpressing human cancer cell lines but not that of the HER2/neu low-expressing human cancer cell lines. Sepharose 124-131 erb-b2 receptor tyrosine kinase 2 Homo sapiens 162-166 16740724-6 2006 Consistent with the apoptosis-inducing activity of T/t-common, we found that T/t-common could specifically inhibit the soft-agarose colony-forming ability of the HER2/neu-overexpressing human cancer cell lines but not that of the HER2/neu low-expressing human cancer cell lines. Sepharose 124-131 erb-b2 receptor tyrosine kinase 2 Homo sapiens 167-170 16793586-5 2006 Glutathione-Sepharose beads were used to purify GST-fusion PAK5-N-terminal fragment. Sepharose 12-21 p21 (RAC1) activated kinase 5 Homo sapiens 59-63 16380266-1 2006 The gene from Escherichia coli encoding aminopeptidase N (PepN) was subcloned into pET-26b, and PepN was over-expressed in BL21(DE3) E. coli and purified using Q-Sepharose chromatography. Sepharose 162-171 aminopeptidase N Escherichia coli 40-56 16621624-4 2006 However, the frequency of high quality stem cells was markedly reduced at the same time, except in cultures containing growth factors and Jagged-1-coated Sepharose-4B beads. Sepharose 154-166 jagged 1 Mus musculus 138-146 16470583-3 2006 In this study a cowpea cystatin, a cysteine proteinase inhibitor found in cowpea (Vigna unguiculata) seeds, was expressed in Escherichia coli and purified with a Ni-NTA agarose column. Sepharose 169-176 cysteine proteinase inhibitor Glycine max 23-31 16585581-6 2006 Using an N-acetylglucosamine (GlcNAc)-agarose column, MBL-like lectin (p25) was isolated from lamprey serum and cDNA cloning was conducted. Sepharose 38-45 mannose binding lectin 2 Homo sapiens 54-57 16755926-3 2006 The expressed hTumstatin was purified to more than 85% purity using a simple one-step SP-Sepharose cation exchange chromatography. Sepharose 89-98 collagen type IV alpha 3 chain Homo sapiens 14-24 16755932-1 2006 Human transferrin receptor (TfR) was isolated from homogenates of placental tissues by affinity chromatography on transferrin-Sepharose, and then used to screen human scFv against it from a fully-synthesized phage scFv library. Sepharose 126-135 transferrin receptor Homo sapiens 6-26 16755932-1 2006 Human transferrin receptor (TfR) was isolated from homogenates of placental tissues by affinity chromatography on transferrin-Sepharose, and then used to screen human scFv against it from a fully-synthesized phage scFv library. Sepharose 126-135 transferrin receptor Homo sapiens 28-31 16755932-1 2006 Human transferrin receptor (TfR) was isolated from homogenates of placental tissues by affinity chromatography on transferrin-Sepharose, and then used to screen human scFv against it from a fully-synthesized phage scFv library. Sepharose 126-135 transferrin Homo sapiens 6-17 16755932-5 2006 The scFv was purified by one-step affinity chromatography with Ni+ -NTA agarose, and injected into Kunming mouse via tail veins. Sepharose 72-79 immunglobulin heavy chain variable region Homo sapiens 4-8 16585581-6 2006 Using an N-acetylglucosamine (GlcNAc)-agarose column, MBL-like lectin (p25) was isolated from lamprey serum and cDNA cloning was conducted. Sepharose 38-45 tubulin polymerization promoting protein Homo sapiens 71-74 16375881-5 2006 eIF4E was purified by affinity chromatography using m(7)GTP-sepharose, and the levels of 4E-BP1 bound to eIF4E were determined. Sepharose 60-69 eukaryotic translation initiation factor 4E Homo sapiens 0-5 16284212-3 2006 beta-Actin was extracted from PMEM lysate with a DNase-Sepharose column. Sepharose 55-64 POTE ankyrin domain family member F Homo sapiens 0-10 16733903-2 2006 METHOD: YAP element was detected by Touchdown PCR amplification and 2% agarose gel electrophoresis. Sepharose 71-78 Yes1 associated transcriptional regulator Homo sapiens 8-11 16542360-8 2006 In IgA1-BP eluted from DesDeGalIgA1/Sepharose, the desialylation of IgA1 was much more pronounced in patients with both pathological phenotypes of IgAN than in normal controls, while the degalactosylation of IgA1 was much more pronounced only in patients with psIgAN than in normal controls. Sepharose 36-45 immunoglobulin heavy constant alpha 1 Homo sapiens 3-7 16542360-8 2006 In IgA1-BP eluted from DesDeGalIgA1/Sepharose, the desialylation of IgA1 was much more pronounced in patients with both pathological phenotypes of IgAN than in normal controls, while the degalactosylation of IgA1 was much more pronounced only in patients with psIgAN than in normal controls. Sepharose 36-45 IGAN1 Homo sapiens 147-151 16542360-9 2006 Furthermore, the amount of IgG in IgA1-BP eluted from DesDeGalIgA1/Sepharose was significantly higher in patients with psIgAN than in normal controls. Sepharose 67-76 immunoglobulin heavy constant alpha 1 Homo sapiens 34-38 16736759-2 2006 Here we immobilized a series of double-stranded DNA probes on an agarose coated slide to investigate the binding affinity of NF-kappaB p50 homodimer to the single-nucleotide mismatches (G<-->A or T<-->C) of the 10 base pair (bp) protein binding sites. Sepharose 65-72 nuclear factor kappa B subunit 1 Homo sapiens 125-138 16544279-1 2006 We have developed a strategy to identify putative tissue-type plasminogen activator (tPA)receptors present in pancreatic cancer cells by affinity capture with tPA-Sepharose followed by 2-DE and MALDI-MS PMF. Sepharose 163-172 plasminogen activator, tissue type Homo sapiens 85-88 16544279-1 2006 We have developed a strategy to identify putative tissue-type plasminogen activator (tPA)receptors present in pancreatic cancer cells by affinity capture with tPA-Sepharose followed by 2-DE and MALDI-MS PMF. Sepharose 163-172 plasminogen activator, tissue type Homo sapiens 159-162 16431918-5 2006 Fluorescent measurements and chromatography studies indicated that FVa709, FVa699, FVa692, and FVa678 bound to FXa membranes and thrombin-agarose in a manner that was comparable with the wild-type cofactors. Sepharose 138-145 coagulation factor II, thrombin Homo sapiens 129-137 16330552-9 2006 Interaction of SP-A and Prdx6 at pH 7.4 was shown by Prdx6-mediated inhibition of SP-A binding to agarose beads, a pull-down assay using His-tagged Prdx6 and Ni(2) -chelating beads, co-immunoprecipitation from lung epithelial cells and from a binary mixture of the two proteins, binding after treatment with a trifunctional cross-linker, and size-exclusion chromatography. Sepharose 98-105 surfactant protein A1 Homo sapiens 15-19 16330552-9 2006 Interaction of SP-A and Prdx6 at pH 7.4 was shown by Prdx6-mediated inhibition of SP-A binding to agarose beads, a pull-down assay using His-tagged Prdx6 and Ni(2) -chelating beads, co-immunoprecipitation from lung epithelial cells and from a binary mixture of the two proteins, binding after treatment with a trifunctional cross-linker, and size-exclusion chromatography. Sepharose 98-105 peroxiredoxin 6 Homo sapiens 24-29 16330552-9 2006 Interaction of SP-A and Prdx6 at pH 7.4 was shown by Prdx6-mediated inhibition of SP-A binding to agarose beads, a pull-down assay using His-tagged Prdx6 and Ni(2) -chelating beads, co-immunoprecipitation from lung epithelial cells and from a binary mixture of the two proteins, binding after treatment with a trifunctional cross-linker, and size-exclusion chromatography. Sepharose 98-105 peroxiredoxin 6 Homo sapiens 53-58 16330552-9 2006 Interaction of SP-A and Prdx6 at pH 7.4 was shown by Prdx6-mediated inhibition of SP-A binding to agarose beads, a pull-down assay using His-tagged Prdx6 and Ni(2) -chelating beads, co-immunoprecipitation from lung epithelial cells and from a binary mixture of the two proteins, binding after treatment with a trifunctional cross-linker, and size-exclusion chromatography. Sepharose 98-105 surfactant protein A1 Homo sapiens 82-86 16330552-9 2006 Interaction of SP-A and Prdx6 at pH 7.4 was shown by Prdx6-mediated inhibition of SP-A binding to agarose beads, a pull-down assay using His-tagged Prdx6 and Ni(2) -chelating beads, co-immunoprecipitation from lung epithelial cells and from a binary mixture of the two proteins, binding after treatment with a trifunctional cross-linker, and size-exclusion chromatography. Sepharose 98-105 peroxiredoxin 6 Homo sapiens 53-58 16426567-2 2006 Using a competition assay on agarose gels we found that the p53 consensus sequences in longer DNA fragments are better targets than the same sequences in shorter DNAs. Sepharose 29-36 tumor protein p53 Homo sapiens 60-63 16402340-7 2006 VNTR polymorphisms of the DAT gene were determined by standard PCR followed by agarose gel electrophoresis. Sepharose 79-86 solute carrier family 6 member 3 Homo sapiens 26-29 16552820-4 2006 The expressed S-protein with Myc/His tag was purified from culture medium with Ni-NTA agarose beads followed by dialysis. Sepharose 86-93 vitronectin Homo sapiens 14-23 23105587-5 2006 We identified a deletion mutation in exon 49 of the dystrophin gene in a 4 yr old boy referred with signs and symptoms suggestive of DMD using primers for exons 45, 48, 49, 43, 44, 19, 3, 8, 13 and muscle promoter, subjected to multiplex polymerase chain reaction (PCR) and agarose/Nu-Sieve gel electrophoresis. Sepharose 274-281 dystrophin Homo sapiens 52-62 16501093-7 2006 Finally, binding of caveolin-1 from mammalian cell lysates to Sepharose-bound, NSP4-specific synthetic peptides confirmed the yeast two-hybrid data and further delineated the binding domain to amino acids 114 to 135. Sepharose 62-71 caveolin 1 Homo sapiens 20-30 16501093-7 2006 Finally, binding of caveolin-1 from mammalian cell lysates to Sepharose-bound, NSP4-specific synthetic peptides confirmed the yeast two-hybrid data and further delineated the binding domain to amino acids 114 to 135. Sepharose 62-71 serine protease 57 Homo sapiens 79-83 16493141-7 2006 PAP binds m(7)GTP-Sepharose and this interaction does not diminish the binding of PAP to purified eIFiso4G, indicating that a complex can form among the cap structure, PAP and eIFiso4G. Sepharose 18-27 poly(A) polymerase alpha Homo sapiens 0-3 16413680-1 2006 A novel phospholipase A2 (PLA2) with Asn at its site 49 was purified from the snake venom of Protobothrops mucrosquamatus by using SP-Sephadex C25, Superdex 75, Heparin-Sepharose (FF) and HPLC reverse-phage C18 chromatography and designated as TM-N49. Sepharose 169-178 phospholipase A2, group IB, pancreas Mus musculus 8-24 16413680-1 2006 A novel phospholipase A2 (PLA2) with Asn at its site 49 was purified from the snake venom of Protobothrops mucrosquamatus by using SP-Sephadex C25, Superdex 75, Heparin-Sepharose (FF) and HPLC reverse-phage C18 chromatography and designated as TM-N49. Sepharose 169-178 phospholipase A2, group IB, pancreas Mus musculus 26-30 16213587-6 2006 Aliquots of NR1, NR2A and NR2B proteins from cell homogenate were immunoprecipitated with protein A agarose and detected by Western blotting. Sepharose 100-107 glutamate receptor, ionotropic, NMDA1 (zeta 1) Mus musculus 12-15 16213587-6 2006 Aliquots of NR1, NR2A and NR2B proteins from cell homogenate were immunoprecipitated with protein A agarose and detected by Western blotting. Sepharose 100-107 glutamate receptor, ionotropic, NMDA2B (epsilon 2) Mus musculus 26-30 16356520-7 2006 Moreover, native KChIP3 from rat brain was to be isolated by beta-Bgt-Sepharose. Sepharose 70-79 potassium voltage-gated channel interacting protein 3 Rattus norvegicus 17-23 16597030-8 2006 Reaction products could be clearly separated with agarose gel and finished in 1 h. CONCLUSION: Modified multiplex PCR protocols supported by EAA and EMQN proved to be very accurate, sensitive and quick, which could be put into screening practice for Y chromosomal microdeletions in AZF region. Sepharose 50-57 AZF1 Homo sapiens 282-285 16310860-7 2006 Ni(2+)-activated IgGs were separated into distinct MBP-hydrolyzing fractions by chromatography on HiTraptrade mark Chelating Sepharose charged with Ni(2+). Sepharose 125-134 myelin basic protein Homo sapiens 51-54 16488976-3 2006 Recombinant IL-32alpha was covalently immobilized on agarose, and preparations of concentrated crude human urinary proteins were applied for chromatographic separation. Sepharose 53-60 interleukin 32 Homo sapiens 12-22 16469315-3 2006 During the purification of EC-SOD from human aorta, we noticed that material with high affinity for heparin-Sepharose formed not only a tetramer but also an octamer. Sepharose 108-117 superoxide dismutase 3 Homo sapiens 27-33 16837900-2 2006 MATERIALS AND METHODS: Cloning and expression of SSX2 antigen in Bac-to-Bac baculovirus expression system as His-tag fusion protein, expression of recombinant SSX2 in insect cells with following purification by affinity chromatography on Ni-NTA agarose, ELISA of blood serum of melanoma patients (n = 29) and healthy donors (n = 27) were used. Sepharose 245-252 SSX family member 2 Homo sapiens 49-53 16837912-4 2006 Antibodies to each hapten were isolated from the serum by affinity chromatography with the hapten-yeast hexokinase-Sepharose sorbents. Sepharose 115-124 hexokinase Saccharomyces cerevisiae S288C 104-114 16216513-3 2006 The compounds synthesized have been tested for their ability to inhibit the ribonucleolytic activity of RNase A by an agarose gel-based assay. Sepharose 118-125 ribonuclease A family member 1, pancreatic Homo sapiens 104-111 16210473-6 2006 In conjunction with molecular chaperones, AP2 and AP1 were recovered from a CK2 phosphorylated agarose-GSH-GST-ASGPR-CD matrix. Sepharose 95-102 transcription factor AP-2 alpha Homo sapiens 42-45 16454826-5 2006 Analysis of the variable number of tandem repeats in intron 2, in its receptor antagonist gene (IL-1RN) was performed by PCR and agarose gel electrophoresis. Sepharose 129-136 interleukin 1 receptor antagonist Homo sapiens 96-102 16364248-4 2006 In this study, the NIH3T3 cells transduced with the exogenous HURP gene manifested the general characteristics of tumor cells, which had higher growth rate in low-serum media and advanced ability of colony formation on agarose-based plates. Sepharose 219-226 DLG associated protein 5 Mus musculus 62-66 16210473-6 2006 In conjunction with molecular chaperones, AP2 and AP1 were recovered from a CK2 phosphorylated agarose-GSH-GST-ASGPR-CD matrix. Sepharose 95-102 FosB proto-oncogene, AP-1 transcription factor subunit Homo sapiens 50-53 16210473-6 2006 In conjunction with molecular chaperones, AP2 and AP1 were recovered from a CK2 phosphorylated agarose-GSH-GST-ASGPR-CD matrix. Sepharose 95-102 asialoglycoprotein receptor 1 Homo sapiens 111-116 16505592-0 2006 The lipoprotein fraction between VLDL and LDL detected by biphasic agarose gel electrophoresis reflects serum remnant lipoprotein and Lp(a) concentrations. Sepharose 67-74 lipoprotein(a) Homo sapiens 134-139 16543664-6 2006 Ca(2+)/calmodulin-dependent cGMP-PDE in rabbit parotid acinar cells was purified using Calmodulin-Sepharose 4B and Mono Q ion-exchange column chromatography. Sepharose 98-110 calmodulin Oryctolagus cuniculus 7-17 16543664-6 2006 Ca(2+)/calmodulin-dependent cGMP-PDE in rabbit parotid acinar cells was purified using Calmodulin-Sepharose 4B and Mono Q ion-exchange column chromatography. Sepharose 98-110 calmodulin Oryctolagus cuniculus 87-97 16202624-4 2006 When mLACTB was expressed as an N-terminal GST fusion protein (GST-mLACTB), full-length GST-mLACTB protein was recovered by glutathione-agarose affinity chromatography as determined by MALDI-TOF mass spectrometry and immunoblotting. Sepharose 136-143 lactamase, beta Mus musculus 67-73 16202624-4 2006 When mLACTB was expressed as an N-terminal GST fusion protein (GST-mLACTB), full-length GST-mLACTB protein was recovered by glutathione-agarose affinity chromatography as determined by MALDI-TOF mass spectrometry and immunoblotting. Sepharose 136-143 lactamase, beta Mus musculus 67-73 16400598-2 2006 CRP was isolated from canine acute-phase serum by affinity chromatography on agarose coupled with phosphorylethanolamine. Sepharose 77-84 C-reactive protein Canis lupus familiaris 0-3 16710996-11 2006 Agarose electrophoresis showed the bands of recombined plasmid pAdeno-Ppp3ca and the recombinant adenovirus identified by enzyme digestion and PCR were in the right range corresponding with expectation. Sepharose 0-7 protein phosphatase 3 catalytic subunit alpha Rattus norvegicus 70-76 16529317-6 2006 RESULTS: The agarose electrophoresis showed that the fragments of BMP-2, pGEM-T and pcDNA3.1(+) were 1.2 kbp, 4.0 kbp and 5.0 kbp, respectively. Sepharose 13-20 bone morphogenetic protein 2 Homo sapiens 66-71 16311081-2 2006 An immunosorbent of polyclonal anti-bovine serum albumin (BSA) antibodies immobilized on CNBR agarose is used in the on-line mode for selective sample pretreatment. Sepharose 94-101 albumin Homo sapiens 43-56 16311081-2 2006 An immunosorbent of polyclonal anti-bovine serum albumin (BSA) antibodies immobilized on CNBR agarose is used in the on-line mode for selective sample pretreatment. Sepharose 94-101 albumin Bos taurus 58-61 16203750-6 2006 CETP was partially purified from lipoprotein-free plasma by FPLC using a Phenyl-Sepharose column. Sepharose 80-89 cholesteryl ester transfer protein Homo sapiens 0-4 16487071-2 2006 In this study, DNase I purification by concanavalin A-wheat germ agglutinin mixture-agarose column from rat (parotid type), rabbit (mixed type), and pig (pancreas type) is described. Sepharose 84-91 deoxyribonuclease-1 Oryctolagus cuniculus 15-22 16099038-7 2006 We demonstrate that BDNF encourages neurite growth into the scaffolds, and reduces further the minimal inflammatory response agarose gels generate in vivo as evidenced by quantitative analysis of the extent of NF-160 kDA positive neurons and axons, GFAP positive reactive astrocytes, and CS-56 positive chondroitin sulfate proteoglycan at the interface of the scaffold and host spinal cord. Sepharose 125-132 brain-derived neurotrophic factor Rattus norvegicus 20-24 16912414-0 2006 Dynamic compression counteracts IL-1beta induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructs. Sepharose 107-114 interleukin 1 beta Homo sapiens 32-40 16912414-0 2006 Dynamic compression counteracts IL-1beta induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructs. Sepharose 107-114 nitric oxide synthase 2 Homo sapiens 49-53 16912414-0 2006 Dynamic compression counteracts IL-1beta induced iNOS and COX-2 activity by human chondrocytes cultured in agarose constructs. Sepharose 107-114 mitochondrially encoded cytochrome c oxidase II Homo sapiens 58-63 16444438-6 2006 In particular, a co-amplified gene on chromosome 2 mimicking a 14 bp deletion in exon 5 of the plasminogen gene was identified by sequencing two different bands obtained from a long run of the PCR exon 5 product in NuSieve agarose gel, and by PstI restriction enzyme analysis of the same amplicons. Sepharose 223-230 plasminogen Homo sapiens 95-106 17087641-3 2006 METHODS: In this study, APOA5 c.553G>T polymorphisms in 152 healthy individuals and 71 type 2 diabetes mellitus patients were detected by PCR-restriction fragment length polymorphism and agarose electrophoresis methods, and serum levels of lipids were also estimated by biochemical methods. Sepharose 190-197 apolipoprotein A5 Homo sapiens 24-29 17211973-7 2006 After RT-PCR (reverse transcriptase polymerase chain reaction), expression of COX I, B2M and VEGF genes was evaluated by agarose gel electrophoresis, visualized by UV illumination. Sepharose 121-128 beta-2 microglobulin Rattus norvegicus 85-88 17211973-7 2006 After RT-PCR (reverse transcriptase polymerase chain reaction), expression of COX I, B2M and VEGF genes was evaluated by agarose gel electrophoresis, visualized by UV illumination. Sepharose 121-128 vascular endothelial growth factor A Rattus norvegicus 93-97 17946858-1 2006 In this study, we report that the sequential application of physiologic deformational loading after culturing with the growth factor TGF-beta3 (for 2-3 weeks) yields significantly stiffer chondrocyte-seeded agarose constructs than cultures in which deformational loading was applied during the initial 2-3 week TGF-beta3 exposure period. Sepharose 207-214 transforming growth factor beta 3 Homo sapiens 133-142 17017928-5 2006 As far as in vitro tests are concerned, IgE assays for cephalosporins, specifically sepharose-radioimmunoassays, are a potentially useful tool in evaluating immediate reactions and could be used as complementary tests. Sepharose 84-93 immunoglobulin heavy constant epsilon Homo sapiens 40-43 17235630-7 2006 The VNTR of IL-1RN was genotyped using the polymerase chain reaction followed by agarose gel electrophoresis. Sepharose 81-88 interleukin 1 receptor antagonist Homo sapiens 12-18 16411604-2 2006 When the interaction of HGF or dHGF with gelatin-immobilized agarose beads was evaluated by Scatchard binding assay, the dissociation constant of dHGF was higher than that of HGF, although the two proteins had a similar binding ratio. Sepharose 61-68 hepatocyte growth factor Mus musculus 24-27 16411604-2 2006 When the interaction of HGF or dHGF with gelatin-immobilized agarose beads was evaluated by Scatchard binding assay, the dissociation constant of dHGF was higher than that of HGF, although the two proteins had a similar binding ratio. Sepharose 61-68 hepatocyte growth factor Mus musculus 32-35 16146697-2 2006 The GST-cyt b5 fusion protein can be matured in vivo as a holoprotein with heme incorporated into cyt b5 during the fermentation, and the purification procedures were simplified by using a one-step affinity column chromatography with glutathione-agarose gel. Sepharose 246-253 cytochrome b5 type A Bos taurus 8-14 16202624-4 2006 When mLACTB was expressed as an N-terminal GST fusion protein (GST-mLACTB), full-length GST-mLACTB protein was recovered by glutathione-agarose affinity chromatography as determined by MALDI-TOF mass spectrometry and immunoblotting. Sepharose 136-143 lactamase, beta Mus musculus 5-11 17046388-5 2006 Purification of different length ataxin-3 variants, including one of pathological length, is facilitated by an N-terminal hexa-histidine tag, which enables binding to a nickel-chelated agarose resin. Sepharose 185-192 ataxin 3 Homo sapiens 33-41 16382178-4 2006 Radioactivity incorporated into GST-SAMS can be recovered easily by precipitation with glutathione-agarose. Sepharose 99-106 methionine adenosyltransferase 1A Homo sapiens 36-40 17062629-8 2006 Agarose gel electrophoresis of genomic DNA isolated from wild-type and chl1 cells, after recovery from MMS treatment, suggested that the wild-type was more proficient in the repair of DNA damage than the mutant. Sepharose 0-7 DNA helicase Saccharomyces cerevisiae S288C 71-75 16211600-5 2006 The activity of eIF4E and its ability to bind mRNA also increases during oocyte maturation as documented in experiments with m7-methyl GTP-Sepharose, which mimics the cap structure of mRNA. Sepharose 139-148 eukaryotic translation initiation factor 4E Sus scrofa 16-21 17684536-4 2006 The fusion protein was then cleaved by thrombin during passing through the GST-agarose 4B column to release the recombinant rat alpha-Syn protein. Sepharose 79-86 coagulation factor II Rattus norvegicus 39-47 17684536-4 2006 The fusion protein was then cleaved by thrombin during passing through the GST-agarose 4B column to release the recombinant rat alpha-Syn protein. Sepharose 79-86 hematopoietic prostaglandin D synthase Rattus norvegicus 75-78 16112873-3 2006 Grx2 is a glutathione binding protein and we have shown in the present study that the protein can be purified from crude bacterial extracts by a one-step affinity chromatography on glutathione-Sepharose. Sepharose 193-202 glutaredoxin 2 Homo sapiens 0-4 16112873-5 2006 However, when Grx2 was fused to either the 27 kDa green fluorescent protein or the 116 kDa beta-galactosidase, the fusion proteins lost their ability to bind glutathione-Sepharose. Sepharose 170-179 glutaredoxin 2 Homo sapiens 14-18 16006145-0 2006 Immobilized beta-cyclodextrin polymer coupled to agarose gel properly refolding recombinant Staphylococcus aureus elongation factor-G in combination with detergent micelle. Sepharose 49-56 AT695_RS01295 Staphylococcus aureus 114-133 16112873-5 2006 However, when Grx2 was fused to either the 27 kDa green fluorescent protein or the 116 kDa beta-galactosidase, the fusion proteins lost their ability to bind glutathione-Sepharose. Sepharose 170-179 galactosidase beta 1 Homo sapiens 91-109 16112873-7 2006 In summary, our findings suggest that Grx2 may be used as an affinity tag for purification of short peptides and possibly also certain proteins that do not interfere with the binding to glutathione-Sepharose. Sepharose 198-207 glutaredoxin 2 Homo sapiens 38-42 16226712-2 2005 Therefore, we isolated MGMT-interacting proteins from extracts of HT29 human colon cancer cells using affinity chromatography on MGMT-Sepharose. Sepharose 134-143 O-6-methylguanine-DNA methyltransferase Homo sapiens 23-27 16253215-7 2005 Recombinant Hu BChE was purified to homogeneity by ammonium sulfate fractionation followed by affinity column chromatography using procainamide Sepharose and cobalt Sepharose gels. Sepharose 144-153 butyrylcholinesterase Homo sapiens 15-19 16253215-7 2005 Recombinant Hu BChE was purified to homogeneity by ammonium sulfate fractionation followed by affinity column chromatography using procainamide Sepharose and cobalt Sepharose gels. Sepharose 165-174 butyrylcholinesterase Homo sapiens 15-19 16330094-12 2005 The most efficient antibody against rpL23 and GFP were purified by Protein G Sepharose affinity chromatography. Sepharose 77-86 ribosomal protein L23 Mus musculus 36-41 17073722-3 2006 For the isolation of RI, DEAE cellulose column chromatography followed by affinity chromatography using CNBr activated Sepharose 4B was performed. Sepharose 119-128 ribonuclease/angiogenin inhibitor 1 Homo sapiens 21-23 20020994-9 2006 Noticeably, the complex has been found to cleave plasmid pBR 322 by agarose gel electrophoresis and cleave CT-DNA by high-performance liquid chromatography (HPLC). Sepharose 68-75 translocator protein Bos taurus 57-60 16406803-7 2005 The resulting recombinant PGIS associates with host cell membranes and was purified to electrophoretic homogeneity by nickel affinity, hydroxyapatite and CM Sepharose column chromatography. Sepharose 157-166 prostaglandin I2 synthase Homo sapiens 26-30 16226712-2 2005 Therefore, we isolated MGMT-interacting proteins from extracts of HT29 human colon cancer cells using affinity chromatography on MGMT-Sepharose. Sepharose 134-143 O-6-methylguanine-DNA methyltransferase Homo sapiens 129-133 16210567-3 2005 METHODS AND RESULTS: Using 5"-biotin-labeled 27nt DNA duplex and streptavidin-agarose beads pull-down assay and mass spectrometry, we identified that nuclear beta-actin was one of the major 27nt binding proteins. Sepharose 78-85 POTE ankyrin domain family member F Homo sapiens 158-168 16297848-7 2005 Immunoblotting of eluates from GSH-Sepharose showed the presence of known (actin, ubiquitin-activating enzyme E1, NF-kappaB, and proteasome) and putative (p53, glutathione-S-transferase P1) targets for glutathionation. Sepharose 35-44 ubiquitin like modifier activating enzyme 7 Homo sapiens 82-112 16297848-7 2005 Immunoblotting of eluates from GSH-Sepharose showed the presence of known (actin, ubiquitin-activating enzyme E1, NF-kappaB, and proteasome) and putative (p53, glutathione-S-transferase P1) targets for glutathionation. Sepharose 35-44 nuclear factor kappa B subunit 1 Homo sapiens 114-123 16418768-2 2005 In the presence of 20 ng/ml TNFalpha, z-VAD-fmk initiated apoptosis and necrosis in the majority of L929 cells as measured by an agarose gel electrophoresis and lactate dehydrogenase(LDH)activity based assay. Sepharose 129-136 tumor necrosis factor Mus musculus 28-36 16236241-0 2005 Assaying Bcr-Abl kinase activity and inhibition in whole cell extracts by phosphorylation of substrates immobilized on agarose beads. Sepharose 119-126 ABL proto-oncogene 1, non-receptor tyrosine kinase Homo sapiens 9-16 16322310-6 2005 Drug-induced formation of cleavable complexes involving topoisomerase IIalpha and topoisomerase IIbeta was evaluated by trapped-in-agarose DNA immunostaining and band depletion assays in the presence and absence of HDACi. Sepharose 131-138 DNA topoisomerase II beta Homo sapiens 56-102 16133495-5 2005 It is concluded that SDS agarose gel electrophoresis and electroelution are suitable methods for studying high molecular weight salivary mucin glycoproteins. Sepharose 25-32 LOC100508689 Homo sapiens 137-142 16385241-0 2005 Interactions of alcohol dehydrogenase to p-hydroxyacetophenone-sepharose and p-acetamidophenol-sepharose. Sepharose 63-72 aldo-keto reductase family 1 member A1 Rattus norvegicus 16-37 16385241-0 2005 Interactions of alcohol dehydrogenase to p-hydroxyacetophenone-sepharose and p-acetamidophenol-sepharose. Sepharose 95-104 aldo-keto reductase family 1 member A1 Rattus norvegicus 16-37 16385241-2 2005 In this study, we investigated ligand specificities to alcohol dehydrogenase (ADH) using p-HAP-sepharose and p-acetamidophenol (AAP)-sepharose. Sepharose 95-104 aldo-keto reductase family 1 member A1 Rattus norvegicus 55-76 16385241-2 2005 In this study, we investigated ligand specificities to alcohol dehydrogenase (ADH) using p-HAP-sepharose and p-acetamidophenol (AAP)-sepharose. Sepharose 95-104 aldo-keto reductase family 1 member A1 Rattus norvegicus 78-81 16393328-4 2005 METHODS: Porcine SP-D was purified from bronchoalveolar lavage fluids of Lan-Yu mini-pigs, by affinity chromatography on maltose-sepharose. Sepharose 129-138 surfactant protein D Sus scrofa 17-21 16708808-3 2005 The transcription and expression of hEndo gene in the transfected melanoma cells were verified by RT-PCR and agarose gel electrophoresis analysis and Western blot. Sepharose 109-116 mannosidase endo-alpha Homo sapiens 36-41 16403267-2 2005 Three monoclonal antibodies against P-glycoprotein (P-gp), the multidrug resistance-associated protein (MRP1) and the breast cancer resistance protein (BCRP) were immobilized onto the agarose gel film-coated glass slides. Sepharose 184-191 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 118-150 16403267-2 2005 Three monoclonal antibodies against P-glycoprotein (P-gp), the multidrug resistance-associated protein (MRP1) and the breast cancer resistance protein (BCRP) were immobilized onto the agarose gel film-coated glass slides. Sepharose 184-191 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 152-156 16412323-5 2005 Leptin gene was genotyped by PCR and agarose electrophoresis. Sepharose 37-44 leptin Homo sapiens 0-6 16336793-0 2005 A method for direct application of human plasmin on a dithiothreitol-containing agarose stacking gel system. Sepharose 80-87 plasminogen Homo sapiens 41-48 16336793-1 2005 A new simplified procedure for identifying human plasmin was developed using a DTT copolymerized agarose stacking gel (ASG) system. Sepharose 97-104 plasminogen Homo sapiens 49-56 15907858-8 2005 Furthermore, upon TSP-1-conjugated Sepharose 4B affinity chromatography, both [125I]Ox-LDL and [125I]latent TGF-beta1 bound to the affinity gel were eluted by unlabeled Ox-LDL. Sepharose 35-44 thrombospondin 1 Homo sapiens 18-23 16251876-8 2005 Clones expressing TbetaRII showed reduced colony formation in soft-agarose assay and significantly reduced tumorigenicity in athymic nude mice. Sepharose 67-74 transforming growth factor, beta receptor II Mus musculus 18-26 15907858-8 2005 Furthermore, upon TSP-1-conjugated Sepharose 4B affinity chromatography, both [125I]Ox-LDL and [125I]latent TGF-beta1 bound to the affinity gel were eluted by unlabeled Ox-LDL. Sepharose 35-44 transforming growth factor beta 1 Homo sapiens 108-117 16000695-4 2005 Using a glutathione S-transferase (GST)-annexin V immobilized sepharose 4B affinity column to trap interacting proteins produced by the GnT-III-transfected M31 cells, we isolated a 47 kDa protein. Sepharose 62-71 beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase Rattus norvegicus 136-143 16100113-9 2005 By affinity chromatography on IgG-Sepharose using Alg14-ZZ as bait, we demonstrate that Alg13-myc co-fractionates with Alg14-ZZ. Sepharose 34-43 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase catalytic subunit ALG13 Saccharomyces cerevisiae S288C 88-93 16150822-4 2005 When placental protein extracts were separated by heparin-Sepharose affinity chromatography, the EL protein eluted as a single peak without detectable phospholipid or triglyceride (TG) lipase activity. Sepharose 58-67 lipase G, endothelial type Homo sapiens 97-99 16126173-2 2005 The trichosanthin-coupled Sepharose affinity purification revealed a protein, which was identified by mass spectrometry as the ribosomal protein L10a. Sepharose 26-35 ribosomal protein L10a Homo sapiens 127-149 16100113-9 2005 By affinity chromatography on IgG-Sepharose using Alg14-ZZ as bait, we demonstrate that Alg13-myc co-fractionates with Alg14-ZZ. Sepharose 34-43 N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase anchoring subunit ALG14 Saccharomyces cerevisiae S288C 119-124 16100120-6 2005 After elution from agarose beads, only one band could be detected by SDS-PAGE, and its identity was confirmed to be LASS5 by mass spectrometry. Sepharose 19-26 ceramide synthase 5 Homo sapiens 116-121 16185077-4 2005 Using a bacterial expression system and ATP-agarose affinity chromatography, we have generated a pure and active recombinant mSR and investigated its substrate and reaction specificity in vitro by analyzing a systematic series of compounds derived from l-Ser and l-serine-O-sulfate. Sepharose 44-51 macrophage scavenger receptor 1 Mus musculus 125-128 16408728-6 2005 Performing the agarose gels electrophoresis with RT-PCR products, compared with the contrast groups, some cells VEGF mRNA of 1366-site were suppressed obviously, the ratio of OD was 0. Sepharose 15-22 vascular endothelial growth factor A Homo sapiens 112-116 16085457-7 2005 The expression of active GST I was established by Western blot analysis, using anti-6His antibody, and by direct purification of 6-His tagged GST I on Ni-NTA agarose. Sepharose 158-165 glutathione S-transferase 1 Zea mays 25-30 16085457-7 2005 The expression of active GST I was established by Western blot analysis, using anti-6His antibody, and by direct purification of 6-His tagged GST I on Ni-NTA agarose. Sepharose 158-165 glutathione S-transferase 1 Zea mays 142-147 16173942-21 2005 The PDZ-RhoGEF, p115RhoGEF and leukaemia-associated RhoGEF (LARG) mRNA expression in coeliac artery was visualized by electrophoresis on agarose gels. Sepharose 137-144 Rho guanine nucleotide exchange factor 12 Rattus norvegicus 60-64 16180102-5 2005 TGF-beta1 increases the binding of laminin gamma1 to WGA-agarose and the binding is abolished by tunicamycin suggesting that laminin gamma1 is modified by N-linked glycosylation. Sepharose 57-64 transforming growth factor, beta 1 Mus musculus 0-9 16180102-5 2005 TGF-beta1 increases the binding of laminin gamma1 to WGA-agarose and the binding is abolished by tunicamycin suggesting that laminin gamma1 is modified by N-linked glycosylation. Sepharose 57-64 laminin, gamma 1 Mus musculus 35-49 16180102-5 2005 TGF-beta1 increases the binding of laminin gamma1 to WGA-agarose and the binding is abolished by tunicamycin suggesting that laminin gamma1 is modified by N-linked glycosylation. Sepharose 57-64 laminin, gamma 1 Mus musculus 125-139 15978327-5 2005 The soluble form of MBP-Ta60b fused scFv could be extracted and affinity-purified with an amylose/agarose column, allowing its immunoreactivity to be analyzed by enzyme-linked immunosorbent assay (ELISA), mixed hemadsorption assay, and fluorescence activated cell sorting. Sepharose 98-105 myelin basic protein Homo sapiens 20-23 16158496-4 2005 Dendritic cells treated with chitosan or PLGA (agarose to a lesser extent) films increased expression levels of CD86, CD40, and HLA-DQ, compared to control iDCs, similar to LPS-matured DCs, whereas DCs treated with alginate or hyaluronic acid films decreased their expression levels of these same molecules. Sepharose 47-54 CD86 molecule Homo sapiens 112-116 16158496-4 2005 Dendritic cells treated with chitosan or PLGA (agarose to a lesser extent) films increased expression levels of CD86, CD40, and HLA-DQ, compared to control iDCs, similar to LPS-matured DCs, whereas DCs treated with alginate or hyaluronic acid films decreased their expression levels of these same molecules. Sepharose 47-54 CD40 molecule Homo sapiens 118-122 16027123-9 2005 Binding assays on heparin-Sepharose showed that, at acidic pH, the triple mutant mGM-CSF binds to immobilized heparin with significantly higher affinity than wild type (WT) mGM-CSF and that neither protein binds to the column at neutral pH. Sepharose 26-35 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 81-88 16039994-6 2005 Heat denatured hResistin abrogated cytokine induction while treatment of recombinant resistin with polymyxin B agarose beads had no effect thereby ruling out the role of endotoxin in the recombinant hResistin mediated cytokine induction. Sepharose 111-118 resistin Homo sapiens 85-93 16144911-6 2005 Serum proteins were fractionated on Sepharose 4B and the activity to elevate IL-6 production was found in the excluded fractions. Sepharose 36-45 interleukin 6 Homo sapiens 77-81 16231848-2 2005 Agarose gel electrophoresis followed by immunofixation is recommended for recognition of an M-protein. Sepharose 0-7 myomesin 2 Homo sapiens 92-101 15880694-5 2005 Agarose gel electrophoresis showed the presence of low-molecular-weight deoxyribonucleic acid (DNA) fragments of adherent wild-type cells cultured with insulin or IGF-I. Sepharose 0-7 insulin-like growth factor 1 Rattus norvegicus 163-168 15890671-7 2005 Enrichment of Cam-interacting proteins using a Cam agarose column revealed that c-Raf kinase forms a complex with Cam. Sepharose 51-58 calmodulin 2 Mus musculus 14-17 15890671-7 2005 Enrichment of Cam-interacting proteins using a Cam agarose column revealed that c-Raf kinase forms a complex with Cam. Sepharose 51-58 calmodulin 2 Mus musculus 47-50 15890671-7 2005 Enrichment of Cam-interacting proteins using a Cam agarose column revealed that c-Raf kinase forms a complex with Cam. Sepharose 51-58 v-raf-leukemia viral oncogene 1 Mus musculus 80-85 15890671-7 2005 Enrichment of Cam-interacting proteins using a Cam agarose column revealed that c-Raf kinase forms a complex with Cam. Sepharose 51-58 calmodulin 2 Mus musculus 47-50 15955834-3 2005 The objective of this study was to examine temporal expression patterns of early responsive genes (Sox9, c-Fos, c-Jun, and TGF-beta type I and II receptors) and induction of their corresponding proteins in agarose culture of rabbit BM-MSCs subjected to cyclic compressive loading. Sepharose 206-213 LOW QUALITY PROTEIN: transcription factor SOX-9 Oryctolagus cuniculus 99-103 15955834-3 2005 The objective of this study was to examine temporal expression patterns of early responsive genes (Sox9, c-Fos, c-Jun, and TGF-beta type I and II receptors) and induction of their corresponding proteins in agarose culture of rabbit BM-MSCs subjected to cyclic compressive loading. Sepharose 206-213 proto-oncogene c-Fos Oryctolagus cuniculus 107-110 15955834-3 2005 The objective of this study was to examine temporal expression patterns of early responsive genes (Sox9, c-Fos, c-Jun, and TGF-beta type I and II receptors) and induction of their corresponding proteins in agarose culture of rabbit BM-MSCs subjected to cyclic compressive loading. Sepharose 206-213 transcription factor Jun Oryctolagus cuniculus 112-117 16143059-3 2005 Glutathione-S-transferase (GST)-hCREG fusion protein was expressed in E.coli BL21 and was used to immunize rabbits to obtain anti-hCREG serum, which was purified by protein A and GST immobilized on glutathione-Sepharose beads. Sepharose 210-219 cellular repressor of E1A stimulated genes 1 Homo sapiens 32-37 16143065-4 2005 The rabbit antibody against mLAIR-1 extracellular region were purified through Sepharose-4B affinity column coupled with mLAIR-1-Fc. Sepharose 79-88 leukocyte-associated Ig-like receptor 1 Mus musculus 28-35 16438848-3 2005 Monoclonal antibodies against P-glycoprotein (P-gP), multidrug resistance-associated protein (MRP1) and breast cancer resistance protein (BCRP) were immobilized onto agarose film-coated glass. Sepharose 166-173 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 138-142 15985216-7 2005 Although bFGF was found to bind more strongly to heparin-Sepharose than endostatin, the latter, but not the former, displaced protamine from heparin in solution, which supports the notion that endostatin can compete with bFGF for binding to heparan sulfate in vivo. Sepharose 57-66 fibroblast growth factor 2 Homo sapiens 9-13 16100443-2 2005 To investigate the mechanism of IL-1beta-induced cell death in human malignant melanoma A375-S2 cells, MTT assay, photomicroscopical observation, DNA agarose gel electrophoresis, radioimmunoassay and Western blot analysis were carried out. Sepharose 150-157 interleukin 1 beta Homo sapiens 32-40 15978327-5 2005 The soluble form of MBP-Ta60b fused scFv could be extracted and affinity-purified with an amylose/agarose column, allowing its immunoreactivity to be analyzed by enzyme-linked immunosorbent assay (ELISA), mixed hemadsorption assay, and fluorescence activated cell sorting. Sepharose 98-105 immunglobulin heavy chain variable region Homo sapiens 36-40 16029448-6 2005 Based on the sensitivity and speed of the bioanalyser, we suggest that this method is invaluable and provides an improvement to the current use of agarose gels for the analysis of FLT3 PCR products. Sepharose 147-154 fms related receptor tyrosine kinase 3 Homo sapiens 180-184 15878877-5 2005 On the other hand, apoA-V-dimyristoylphosphatidylcholine disc particles bound to heparin-Sepharose and were specifically eluted upon application of a linear gradient of NaCl. Sepharose 89-98 apolipoprotein A5 Homo sapiens 19-25 16080722-2 2005 Fab fragments were obtained by digestion with papain of anti-SEB IgG bound to SEB immobilized on Sepharose 4B. Sepharose 97-106 FA complementation group B Homo sapiens 0-3 16080722-4 2005 SEB-Sepharose was used repeatedly for the production of anti-SEB Fab fragments by papain digestion without loss of affinity. Sepharose 4-13 FA complementation group B Homo sapiens 65-68 15939289-8 2005 The secreted EDN was purified to a purity of 98 % by the use of SP Sepharose FF ion-exchange chromatography and Sepharose-heparin Hi Trap affinity chromatography. Sepharose 67-76 collagen, type XVIII, alpha 1 Mus musculus 13-16 15968536-8 2005 The PCR products for the TLR-2 gene were analysed on 1.5% agarose gel pre-stained with ethidium bromide. Sepharose 58-65 toll like receptor 2 Homo sapiens 25-30 15696117-8 2005 The expressions of MMP-7 were further confirmed by agarose gel electrophoresis and Western blotting. Sepharose 51-58 matrix metallopeptidase 7 Homo sapiens 19-24 15939289-8 2005 The secreted EDN was purified to a purity of 98 % by the use of SP Sepharose FF ion-exchange chromatography and Sepharose-heparin Hi Trap affinity chromatography. Sepharose 112-121 collagen, type XVIII, alpha 1 Mus musculus 13-16 16029163-3 2005 Purified His-hSR was refolded in Tween 20/cycloamylose with approximately 50% efficiency, and refolded His-hSR was isolated by Q Sepharose column chromatography. Sepharose 129-138 HSR Homo sapiens 107-110 16176092-5 2005 20mg of beta3 peptide was obtained from one litter culture medium after purification by using metal-chelating sepharose 6B FF. Sepharose 110-119 eukaryotic translation elongation factor 1 beta 2 pseudogene 2 Homo sapiens 8-13 15924430-15 2005 Tear fluid PLTP was quantitatively bound to Heparin-Sepharose and could be eluted as a single peak by 0.5 M NaCl. Sepharose 52-61 phospholipid transfer protein Homo sapiens 11-15 16038296-1 2005 This work provides a theoretical analysis of multicomponent adsorption kinetics for conditions typical of protein adsorption in porous ion exchangers as well as experimental results for the adsorption of lysozyme/cytochrome c mixtures in the cation exchanger SP-Sepharose-FF. Sepharose 262-271 lysozyme Homo sapiens 204-212 15737636-5 2005 First, angiogenin was pulled down together with His-tagged alpha-actinin-2 by Ni(2+)-agarose resins. Sepharose 85-92 angiogenin Homo sapiens 7-17 16006196-2 2005 G6PD was purified from erythrocytes with a specific activity of 16.7 EU (mmol NADP+/min)/mg protein and approximately 1600-fold in a yield of approximately 60% by ammonium sulphate precipitation and 2",5"-ADP Sepharose 4B affinity chromatography. Sepharose 209-221 glucose-6-phosphate-1-dehydrogenase Oncorhynchus mykiss 0-4 16027777-7 2005 Intracellularly expressed Gag was released by high-pressure homogenization and purified through Sepharose FF and DEAE Sepharose FF column chromatography, the purity of Gag reached up to 90%. Sepharose 96-105 Pr55(Gag) Human immunodeficiency virus 1 26-29 16038263-3 2005 The recombinant protein PEDF was expressed in E.coli BL-21, and purified by the GST Sepharose 4B affinity column. Sepharose 84-93 serpin family F member 1 Homo sapiens 24-28 15617517-3 2005 Here we provide evidence that Ixolaris binds specifically to the FXa HBE (heparin-binding exosite), since (i) it markedly decreases the inhibition of FXa by the antithrombin-heparin but not the antithrombin-pentasaccharide complex, (ii) it impairs FXa binding to Sepharose-immobilized heparin, and (iii) it allosterically modulates the catalytic activity of FXa for small chromogenic substrates (S-2765). Sepharose 263-272 coagulation factor X Homo sapiens 65-68 21638583-6 2005 PCRs using primers directed to two different regions of the unc-93 gene are carried out on the genomic DNA from wild-type and mutant strains, and the PCR products are analyzed by agarose gel electrophoresis. Sepharose 179-186 Putative potassium channel regulatory protein unc-93 Caenorhabditis elegans 60-66 15827963-3 2005 We here demonstrate statistically significant synergy between regakine-1 and the neutrophil attractants C5a or IL-8/CXCL8 in inducing neutrophil shape change and migration under agarose. Sepharose 178-185 hemolytic complement Mus musculus 104-107 15827963-3 2005 We here demonstrate statistically significant synergy between regakine-1 and the neutrophil attractants C5a or IL-8/CXCL8 in inducing neutrophil shape change and migration under agarose. Sepharose 178-185 chemokine (C-X-C motif) ligand 15 Mus musculus 111-115 15827963-3 2005 We here demonstrate statistically significant synergy between regakine-1 and the neutrophil attractants C5a or IL-8/CXCL8 in inducing neutrophil shape change and migration under agarose. Sepharose 178-185 C-X-C motif chemokine ligand 8 Homo sapiens 116-121 15845487-7 2005 Using hSP-A-coupled Sepharose affinity chromatography and polyacrylamide gel electrophoresis, we identified a 65-kDa hSP-A binding protein of M. pneumoniae. Sepharose 20-29 heat shock protein 90 beta family member 2, pseudogene Homo sapiens 6-9 15845487-7 2005 Using hSP-A-coupled Sepharose affinity chromatography and polyacrylamide gel electrophoresis, we identified a 65-kDa hSP-A binding protein of M. pneumoniae. Sepharose 20-29 heat shock protein 90 beta family member 2, pseudogene Homo sapiens 117-120 15858705-10 2005 METHODS: Lectin containing agarose gel for AFP electrophoresis leads to AFP separation according to different affinities of the varying carbohydrate chains of AFP to lectins. Sepharose 27-34 alpha fetoprotein Homo sapiens 43-46 15858705-10 2005 METHODS: Lectin containing agarose gel for AFP electrophoresis leads to AFP separation according to different affinities of the varying carbohydrate chains of AFP to lectins. Sepharose 27-34 alpha fetoprotein Homo sapiens 72-75 15858705-10 2005 METHODS: Lectin containing agarose gel for AFP electrophoresis leads to AFP separation according to different affinities of the varying carbohydrate chains of AFP to lectins. Sepharose 27-34 alpha fetoprotein Homo sapiens 72-75 15862137-3 2005 The expressed IL-24-GST fusion protein was purified via GST-Sepharose 4B Column and identified by SDS-PAGE and Western blot. Sepharose 60-69 interleukin 24 Homo sapiens 14-19 15831250-4 2005 Agarose based chromatography matrices showed high capacities for the denatured alpha-lactalbumin. Sepharose 0-7 lactalbumin alpha Bos taurus 79-96 15737636-5 2005 First, angiogenin was pulled down together with His-tagged alpha-actinin-2 by Ni(2+)-agarose resins. Sepharose 85-92 actinin alpha 2 Homo sapiens 59-74 15814629-3 2005 Amplified ACE gene fragments were separated on agarose gels. Sepharose 47-54 angiotensin I converting enzyme Homo sapiens 10-13 15861784-6 2005 After incubation, the complex was trapped by annealing the (GT)5 tail of the C/EBP-[ACEP24(GT)5] complex to an (AC)5-Sepharose column under flow at 4 degrees C. The column was washed with 0.4 M NaCl and the protein eluted with 1.2 M NaCl. Sepharose 117-126 CCAAT/enhancer binding protein gamma Rattus norvegicus 77-82 15805962-6 2005 Interleukin-1 beta is a powerful catabolic agent; in unstrained agarose-chondrocyte constructs, interleukin-1 beta caused a decrease in the mRNA levels of anabolic factors. Sepharose 64-71 interleukin 1 beta Bos taurus 96-114 15794764-4 2005 The rPf1-Cys-Prx bound to FP-agarose at pH 7.4, which is the pH of the parasite cytosol. Sepharose 29-36 ribosome production factor 1 Rattus norvegicus 4-8 15784557-7 2005 Depletion of SP-D with mannan-conjugated Sepharose or anti-SP-D antibody reduced the protective effect of tears against P. aeruginosa invasion. Sepharose 41-50 surfactant protein D Homo sapiens 13-17 15781109-1 2005 C1-esterase inhibitor (C1-INH) was depleted from normal human serum (NHS) at 4 degrees C by affinity chromatography with a monoclonal anti-C1-INH antibody (mAb 13 E1) coupled to CNBr-activated Sepharose 4B. Sepharose 193-202 serpin family G member 1 Homo sapiens 23-29 16022192-1 2005 The objective of the present study was to establish whether high-density lipoprotein 3 (HDL3) or high-density lipoprotein 2 (HDL2) might show an anti-oxidative effect on the acceleration of the oxidative modification of low-density lipoprotein (LDL) by ascorbic acid from measurement of the agarose gel electrophoretic mobility of LDL. Sepharose 291-298 HDL3 Homo sapiens 60-86 16022192-1 2005 The objective of the present study was to establish whether high-density lipoprotein 3 (HDL3) or high-density lipoprotein 2 (HDL2) might show an anti-oxidative effect on the acceleration of the oxidative modification of low-density lipoprotein (LDL) by ascorbic acid from measurement of the agarose gel electrophoretic mobility of LDL. Sepharose 291-298 HDL3 Homo sapiens 88-92 15659642-6 2005 Overexpression of Mystique 2 in MCF-7 cells suppressed colony formation in soft agarose and enhanced cell adhesion to collagen and fibronectin. Sepharose 80-87 PDZ and LIM domain 2 Homo sapiens 18-26 15766874-0 2005 A mild hydrophobic interaction chromatography involving polyethylene glycol immobilized to agarose media refolding recombinant Staphylococcus aureus elongation factor G. Sepharose 91-98 AT695_RS01295 Staphylococcus aureus 149-168 15389646-6 2005 In order to test if FBPase interacts with aldolase B, FBPase was immobilized on agarose and subjected to binding experiments. Sepharose 80-87 fructose-bisphosphatase 2 Rattus norvegicus 54-60 15591041-7 2005 Moreover, LC2 enhanced the ability of transfected and endogenous EPAC1 to interact with cyclic AMP-agarose, indicating that LC2 elicits conformational changes in the cAMP domain of EPAC1, enhancing its ability to be activated by cyclic AMP. Sepharose 99-106 Rap guanine nucleotide exchange factor 3 Rattus norvegicus 65-70 15591041-7 2005 Moreover, LC2 enhanced the ability of transfected and endogenous EPAC1 to interact with cyclic AMP-agarose, indicating that LC2 elicits conformational changes in the cAMP domain of EPAC1, enhancing its ability to be activated by cyclic AMP. Sepharose 99-106 Rap guanine nucleotide exchange factor 3 Rattus norvegicus 181-186 15537792-2 2005 We isolated the XEEL protein from the extract of tailbud embryos by affinity chromatography on a galactose-Sepharose column. Sepharose 107-116 intelectin 1 L homeolog Xenopus laevis 16-20 15865443-6 2005 Incubation of Sepharose bead-bound GST-TNF-R1(207)(-)(425).4D/E fusion protein with lysates containing activated p42(mapk/erk2) led to the phosphorylation of Ser and Thr residues in addition to the previously defined sites at T236, S240, S244, and S270. Sepharose 14-23 TNF receptor superfamily member 1A Homo sapiens 39-45 15865443-6 2005 Incubation of Sepharose bead-bound GST-TNF-R1(207)(-)(425).4D/E fusion protein with lysates containing activated p42(mapk/erk2) led to the phosphorylation of Ser and Thr residues in addition to the previously defined sites at T236, S240, S244, and S270. Sepharose 14-23 cyclin dependent kinase 20 Homo sapiens 113-116 15865443-6 2005 Incubation of Sepharose bead-bound GST-TNF-R1(207)(-)(425).4D/E fusion protein with lysates containing activated p42(mapk/erk2) led to the phosphorylation of Ser and Thr residues in addition to the previously defined sites at T236, S240, S244, and S270. Sepharose 14-23 mitogen-activated protein kinase 1 Homo sapiens 122-126 15670615-8 2005 The specific amplified band of GnRH and its receptor sequences were detected through Agarose gel electrophoresis, and GnRH gene sequence is identical to that of GnRH which has been reported in rat hypothalamus and GnRH receptor sequence is identical to that of the pituitary of rat. Sepharose 85-92 gonadotropin releasing hormone 1 Rattus norvegicus 31-35 15804188-8 2005 DNA sequencing and agarose gel electrophoresis confirmed the successful cloning of the monomer gene segment of SELP415K consisting of 312 bp. Sepharose 19-26 selectin P Homo sapiens 111-115 15686475-7 2005 Thirdly, results from calmodulin-agarose affinity column chromatography studies indicated a direct interaction or binding between ApoCaM and GAD. Sepharose 33-40 glutamate decarboxylase 1 Homo sapiens 141-144 15632291-6 2005 When lysates from NIH3T3 cells were incubated with calmodulin-agarose beads in the presence of CaCl(2) or EGTA, calcium ion drastically enhanced the interaction between Nef and calmodulin, suggesting that the binding is under the influence of Ca(2+) signaling. Sepharose 62-69 calmodulin 2 Mus musculus 51-61 15632291-6 2005 When lysates from NIH3T3 cells were incubated with calmodulin-agarose beads in the presence of CaCl(2) or EGTA, calcium ion drastically enhanced the interaction between Nef and calmodulin, suggesting that the binding is under the influence of Ca(2+) signaling. Sepharose 62-69 TNFAIP3 interacting protein 1 Mus musculus 169-172 15632291-6 2005 When lysates from NIH3T3 cells were incubated with calmodulin-agarose beads in the presence of CaCl(2) or EGTA, calcium ion drastically enhanced the interaction between Nef and calmodulin, suggesting that the binding is under the influence of Ca(2+) signaling. Sepharose 62-69 calmodulin 2 Mus musculus 177-187 15842803-2 2005 METHODS: Four different p53-GST (glutathione S-transferase) fusion proteins and GST were expressed in E. coli and purified through glutathione sepharose 4B beads. Sepharose 143-152 tumor protein p53 Homo sapiens 24-27 15637741-3 2005 Ep-CAM-GST fusion protein was induced by isopropyl-beta-D-thiogalactopyranoside (IPTG) and purified with glutathione-sepharose. Sepharose 117-126 epithelial cell adhesion molecule Homo sapiens 0-6 16496609-13 2005 Genotypes for ACE I/D and PAI-1 4G/5G polymorphisms were determined with the polymerase chain reaction amplification followed by electrophoresis of the product on an agarose gel and detection with ethidium bromide. Sepharose 166-173 serpin family E member 1 Homo sapiens 26-31 15816554-1 2005 BACKGROUND: The aim of our study was to detect Ep-CAM mRNA in peripheral blood (PB) and bone marrow (BM) samples of patients with metastatic colorectal cancer, using a novel chromatographic method (molecular strip) for the detection of PCR amplified product and to confirm the results by the standard method of agarose gel electrophoresis. Sepharose 311-318 epithelial cell adhesion molecule Homo sapiens 47-53 15942167-5 2005 METHODS: The scFv was immobilized onto agarose and characterized for beta2m binding capacity, thermal stability at 37 degrees C, regeneration capacity, storage conditions, and sterility. Sepharose 39-46 immunglobulin heavy chain variable region Homo sapiens 13-17 15606797-4 2005 Porcine SP-D was purified from porcine bronchoalveolar lavage (BAL) by maltose-agarose and immunoglobulin M affinity chromatography. Sepharose 79-86 surfactant protein D Homo sapiens 8-12 15677791-6 2005 The I/D polymorphism of the ACE gene was examined by PCR followed by agarose gel electrophoresis. Sepharose 69-76 angiotensin I converting enzyme Homo sapiens 28-31 15864429-5 2005 The MBP-tagged enzyme was efficiently purified by a combination of cation-exchange column and amylase-conjugated agarose column chromatography. Sepharose 113-120 myelin basic protein Homo sapiens 4-7 15565646-3 2005 The interaction of CK1alpha from Danio rerio with mouse-axin has been studied using a pull-down assay that uses fragments of axin fused to glutathione S transferase, which is bound to glutathione sepharose beads. Sepharose 196-205 axin 1 Mus musculus 56-60 15565646-3 2005 The interaction of CK1alpha from Danio rerio with mouse-axin has been studied using a pull-down assay that uses fragments of axin fused to glutathione S transferase, which is bound to glutathione sepharose beads. Sepharose 196-205 axin 1 Mus musculus 125-129 16491431-2 2005 At least five size classes of titin isoforms have been identified using SDS agarose gel electrophoresis. Sepharose 76-83 titin Rattus norvegicus 30-35 15593393-2 2005 METHODS: MBL was purified from human serum by affinity chromatography on mannan-Sepharose 4B column. Sepharose 80-89 mannose binding lectin 2 Homo sapiens 9-12 15782940-8 2005 The capacity of the recombinant protein VirE2 for interacting with single-stranded DNA was tested by the formation of complexes, recorded by agarose-gel electrophoresis. Sepharose 141-148 type IV secretion system single-stranded DNA binding protein VirE2 Agrobacterium tumefaciens 40-45 15835784-8 2005 The ability of the recombinant protein VirE2 to bind to single-stranded DNA was judged from the formation of complexes detected by electrophoresis in agarose gel. Sepharose 150-157 type IV secretion system single-stranded DNA binding protein VirE2 Agrobacterium tumefaciens 39-44 16521944-7 2005 The common deletions of GSTM1 and GSTT1 were determined by polymerase chain reaction and agarose gel separation. Sepharose 89-96 glutathione S-transferase mu 1 Homo sapiens 24-29 15613999-7 2005 RANK-L expression in each periapical sample was assessed by agarose gel electrophoresis. Sepharose 60-67 TNF superfamily member 11 Homo sapiens 0-6 16521944-7 2005 The common deletions of GSTM1 and GSTT1 were determined by polymerase chain reaction and agarose gel separation. Sepharose 89-96 glutathione S-transferase theta 1 Homo sapiens 34-39 15381692-2 2004 We purified ADAM 12 from the urine of breast cancer patients via Q-Sepharose anion exchange and gelatin-Sepharose affinity chromatography followed by protein identification by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Sepharose 67-76 ADAM metallopeptidase domain 12 Homo sapiens 12-19 15551052-0 2005 In vitro assessment of a new ABO immunosorbent with synthetic carbohydrates attached to sepharose. Sepharose 88-97 ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Homo sapiens 29-32 15551052-4 2005 A new ABO immunosorbent with synthetic A- or B-trisaccharide carbohydrate epitopes linked to a sepharose matrix has been tested. Sepharose 95-104 ABO, alpha 1-3-N-acetylgalactosaminyltransferase and alpha 1-3-galactosyltransferase Homo sapiens 6-9 15381692-2 2004 We purified ADAM 12 from the urine of breast cancer patients via Q-Sepharose anion exchange and gelatin-Sepharose affinity chromatography followed by protein identification by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Sepharose 104-113 ADAM metallopeptidase domain 12 Homo sapiens 12-19 15546023-7 2004 Ladder-like patterns of DNA fragments were observed in PC-3m cells treated with 10 mmol/L antisense HSP70 oligomer for 48 hr or 8 mmol/L for 72 hr on agarose gel electrophoresis. Sepharose 150-157 heat shock protein family A (Hsp70) member 4 Homo sapiens 100-105 15619032-8 2004 In vitro affinity assays showed specific interactions of recombinant actinin-4 R1-R4, R1, R2, R3, and R4 proteins with humanin-Sepharose. Sepharose 127-136 actinin alpha 4 Homo sapiens 69-78 15545387-8 2004 Agarose gel electrophoresis of the lactase PCR product revealed a single band that corresponded to the single-amplified product as predicted by the melting curve analysis of the PCR. Sepharose 0-7 lactase Homo sapiens 35-42 15604061-2 2004 METHODS: A two-step affinity chromatography on underivatized sepharose 4B was employed for purification of MBL-MASP complex, followed by gel filtration on a Sephacryl S-300 column for separation of MBL and MASPs from the complex. Sepharose 61-70 mannose binding lectin 2 Homo sapiens 107-110 15385564-3 2004 Evidence that LPL dimers rapidly exchange subunits was demonstrated by fluorescence resonance energy transfer between lipase subunits labeled with Oregon Green and tetrametylrhodamine, respectively, and also by formation of heterodimers composed of radiolabeled and biotinylated lipase subunits captured on streptavidine-agarose. Sepharose 321-328 lipoprotein lipase Homo sapiens 14-17 15562000-7 2004 Isolation of LacI-SC35 was also accomplished by using biotinylated dsDNA and streptavidin sepharose. Sepharose 90-99 tissue factor pathway inhibitor Homo sapiens 13-17 15368304-4 2004 Upon purification of chlamydial HSP60 with polymyxin B-agarose columns, its ability to induce TNF secretion in vitro is much reduced. Sepharose 55-62 heat shock protein family D (Hsp60) member 1 Homo sapiens 32-37 15539052-3 2004 Induction of apoptosis in pTet-on-LMP1 HNE2 cells after the EGCG treatment was analyzed by agarose gel electrophoresis. Sepharose 91-98 PDZ and LIM domain 7 Homo sapiens 34-38 15505035-0 2004 Identification of target genes regulated by FOXC1 using nickel agarose-based chromatin enrichment. Sepharose 63-70 forkhead box C1 Homo sapiens 44-49 15454261-2 2004 Titin from 16-day fetal hearts consisted of a single 3710 kDa band on SDS agarose gels, and it disappeared by 10 days after birth. Sepharose 74-81 titin Rattus norvegicus 0-5 15319442-4 2004 A Ca2+ -dependent interaction occurs between calmodulin-linked agarose and the holo-V2R as well as the V2R C terminus. Sepharose 63-70 calmodulin-2 Canis lupus familiaris 45-55 15319442-4 2004 A Ca2+ -dependent interaction occurs between calmodulin-linked agarose and the holo-V2R as well as the V2R C terminus. Sepharose 63-70 arginine vasopressin receptor 2 Canis lupus familiaris 84-87 15319442-6 2004 NMR studies showed that a peptide fragment of the V2R C terminus containing the RGR sequence binds to calmodulin in a Ca2+ -dependent manner with a Kd < or =1.5 microm; concentration-dependent binding of the V2R C terminus to calmodulin-agarose was used to estimate a Kd value of approximately 200 nm for this entire C-terminal sequence as expressed in mammalian cells. Sepharose 240-247 arginine vasopressin receptor 2 Canis lupus familiaris 50-53 15319442-6 2004 NMR studies showed that a peptide fragment of the V2R C terminus containing the RGR sequence binds to calmodulin in a Ca2+ -dependent manner with a Kd < or =1.5 microm; concentration-dependent binding of the V2R C terminus to calmodulin-agarose was used to estimate a Kd value of approximately 200 nm for this entire C-terminal sequence as expressed in mammalian cells. Sepharose 240-247 retinal G protein coupled receptor Homo sapiens 80-83 15319442-6 2004 NMR studies showed that a peptide fragment of the V2R C terminus containing the RGR sequence binds to calmodulin in a Ca2+ -dependent manner with a Kd < or =1.5 microm; concentration-dependent binding of the V2R C terminus to calmodulin-agarose was used to estimate a Kd value of approximately 200 nm for this entire C-terminal sequence as expressed in mammalian cells. Sepharose 240-247 calmodulin 1 Homo sapiens 102-112 15319442-6 2004 NMR studies showed that a peptide fragment of the V2R C terminus containing the RGR sequence binds to calmodulin in a Ca2+ -dependent manner with a Kd < or =1.5 microm; concentration-dependent binding of the V2R C terminus to calmodulin-agarose was used to estimate a Kd value of approximately 200 nm for this entire C-terminal sequence as expressed in mammalian cells. Sepharose 240-247 arginine vasopressin receptor 2 Canis lupus familiaris 211-214 15464999-8 2004 It was confirmed by CaM-agarose column chromatography that the dissociation of nNOS-CaM complex was induced by organotin compounds. Sepharose 24-31 nitric oxide synthase 1 Rattus norvegicus 79-83 15560139-3 2004 We have now re-engineered the S-peptide/S-protein system to allow isolation of S-peptide-tagged polypeptides and their binding partners from eukaryotic cells with S-protein-agarose. Sepharose 173-180 vitronectin Homo sapiens 40-49 15560139-3 2004 We have now re-engineered the S-peptide/S-protein system to allow isolation of S-peptide-tagged polypeptides and their binding partners from eukaryotic cells with S-protein-agarose. Sepharose 173-180 vitronectin Homo sapiens 163-172 15387863-6 2004 The C677T transition in the MTHFR gene was detected by HinfI restriction enzyme analysis and subsequent electrophoresis in a 3% agarose gel. Sepharose 128-135 methylenetetrahydrofolate reductase Homo sapiens 28-33 15368365-1 2004 We previously reported that a complex of nuclear proteins from HeLa cells, among them histone H1 and casein kinase 2 co-eluted from immobilized nucleosome assembly protein 2 (NAP-2)-Sepharose. Sepharose 182-191 H1.0 linker histone Homo sapiens 86-96 15368365-1 2004 We previously reported that a complex of nuclear proteins from HeLa cells, among them histone H1 and casein kinase 2 co-eluted from immobilized nucleosome assembly protein 2 (NAP-2)-Sepharose. Sepharose 182-191 nucleosome assembly protein 1 like 4 Homo sapiens 144-173 15368365-1 2004 We previously reported that a complex of nuclear proteins from HeLa cells, among them histone H1 and casein kinase 2 co-eluted from immobilized nucleosome assembly protein 2 (NAP-2)-Sepharose. Sepharose 182-191 nucleosome assembly protein 1 like 4 Homo sapiens 175-180 15368366-10 2004 We expressed these human Gsalpha (hGsalpha) mutants in bacteria as histidine tagged proteins, purified them by niquel-agarose chromatography and studied their nucleotide exchange properties. Sepharose 118-125 GNAS complex locus Homo sapiens 25-32 15368366-10 2004 We expressed these human Gsalpha (hGsalpha) mutants in bacteria as histidine tagged proteins, purified them by niquel-agarose chromatography and studied their nucleotide exchange properties. Sepharose 118-125 GNAS complex locus Homo sapiens 34-42 15358369-4 2004 By using the Sepharose-IDA-Zn(2+) affinity column, we purified the Escherichia coli expressed hCA-II with an overall recovery of 76%. Sepharose 13-22 carbonic anhydrase 2 Homo sapiens 94-100 15623158-4 2004 Bioassay using CaM antagonists W7-agarose and anti-CaM serum shows that the endogenous extracellular CaM promotes stomatal closure and delays stomatal opening. Sepharose 34-41 calmodulin 1 Homo sapiens 15-18 15498136-8 2004 The result showed that after the pDs-red2-N1/ALAS2 eukaryotic expression vector was digested by KpnI and BamHI, two fragments of 4 700 bp and 1 764 bp were displayed by electrophoresis on agarose gel. Sepharose 188-195 5'-aminolevulinate synthase 2 Homo sapiens 45-50 15730649-4 2004 The DNA ladder strips were observed with agarose electrophoresis at 12th and 24th PTH. Sepharose 41-48 parathyroid hormone Homo sapiens 82-85 15374620-7 2004 Heparin-sepharose (100 mM NaCl eluate)-purified preparation contained the MMP-2/TIMP-2 complex. Sepharose 8-17 matrix metallopeptidase 2 Bos taurus 74-79 15362892-5 2004 Second, a peptide corresponding to the Arg-rich I3 region of the D2R (215VLRRRRKRVN224) and bound to Sepharose was able to pull down both GST-A2ACT and the whole A2AR solubilized from A2AR-tranfected HEK-293 cells. Sepharose 101-110 adenosine A2a receptor Homo sapiens 162-166 15362892-5 2004 Second, a peptide corresponding to the Arg-rich I3 region of the D2R (215VLRRRRKRVN224) and bound to Sepharose was able to pull down both GST-A2ACT and the whole A2AR solubilized from A2AR-tranfected HEK-293 cells. Sepharose 101-110 adenosine A2a receptor Homo sapiens 184-188 15466886-4 2004 beta1-Integrin bound to a midkine-agarose column and was eluted mostly with EDTA. Sepharose 34-41 midkine Mus musculus 26-33 15362864-2 2004 Mannan-agarose purified RK(13) cell CRT (rCRT) selectively bound sepharose-immobilized L,D-MDP and PG, but not L,L-MDP or D,D-MDP. Sepharose 7-14 calreticulin Oryctolagus cuniculus 36-39 15362864-2 2004 Mannan-agarose purified RK(13) cell CRT (rCRT) selectively bound sepharose-immobilized L,D-MDP and PG, but not L,L-MDP or D,D-MDP. Sepharose 65-74 calreticulin Oryctolagus cuniculus 36-39 15258159-1 2004 Rhodopsin (Rho) has been extracted in n-dodecyl beta-D-maltoside (DM) from bovine retinal rod outer segments and purified to homogeneity by affinity chromatography on concanavalin A-Sepharose. Sepharose 182-191 rhodopsin Bos taurus 0-9 15363631-3 2004 Dictyostelium, rabbit and yeast PGKs bound to calmodulin-agarose in a calcium-dependent manner while DdPGK constructs lacking the calmodulin-binding domain (209KPFLAILGGAKVSDKIKLIE228) failed to bind. Sepharose 57-64 calmodulin Saccharomyces cerevisiae S288C 46-56 15220352-11 2004 Furthermore, we observed that RhoA activity after stimulation with soluble Thy-1-Fc molecule was augmented upon further cross-linking using protein A-Sepharose beads. Sepharose 150-159 ras homolog family member A Homo sapiens 30-34 15220352-11 2004 Furthermore, we observed that RhoA activity after stimulation with soluble Thy-1-Fc molecule was augmented upon further cross-linking using protein A-Sepharose beads. Sepharose 150-159 Thy-1 cell surface antigen Homo sapiens 75-80 15358533-3 2004 A peptide (268E-W348) corresponding to 81 aa of the small cytoplasmic loop of At-ACA8 binds peptide 6His-1M-I116 immobilized on Ni-NTA agarose. Sepharose 135-142 autoinhibited Ca2+ -ATPase Arabidopsis thaliana 81-85 15353038-6 2004 Based on these studies a rAAV5-CB-promoter-driven CFTR minigene vector was then used to correct the CF chloride transport defect in vitro, as well as the hyperinflammatory lung phenotype in Pseudomonas-agarose bead challenged CF mouse lungs in vivo. Sepharose 202-209 cystic fibrosis transmembrane conductance regulator Mus musculus 50-54 15593035-0 2004 Compositional similarity between immunoglobulins binding to asialo-, agalacto-IgA1-Sepharose and those deposited in glomeruli in IgA nephropathy. Sepharose 83-92 immunoglobulin heavy constant alpha 1 Homo sapiens 78-82 15593035-0 2004 Compositional similarity between immunoglobulins binding to asialo-, agalacto-IgA1-Sepharose and those deposited in glomeruli in IgA nephropathy. Sepharose 83-92 immunoglobulin heavy variable 4-38-2-like Homo sapiens 78-81 15593035-1 2004 BACKGROUND: It has been found that the immunoglobulin A1-binding protein (IgA1-BP) can be separated from human serum using an asialo-, agalacto-IgA1 (aglyco-IgA1)-Sepharose column (13). Sepharose 163-172 immunoglobulin heavy constant alpha 1 Homo sapiens 74-78 15593035-1 2004 BACKGROUND: It has been found that the immunoglobulin A1-binding protein (IgA1-BP) can be separated from human serum using an asialo-, agalacto-IgA1 (aglyco-IgA1)-Sepharose column (13). Sepharose 163-172 immunoglobulin heavy constant alpha 1 Homo sapiens 144-148 15593035-1 2004 BACKGROUND: It has been found that the immunoglobulin A1-binding protein (IgA1-BP) can be separated from human serum using an asialo-, agalacto-IgA1 (aglyco-IgA1)-Sepharose column (13). Sepharose 163-172 immunoglobulin heavy constant alpha 1 Homo sapiens 144-148 15593035-3 2004 METHODS: IgA1-BP was separated from serum using the aglyco-IgA1-Sepharose column and the aglyco-IgA1-HPLC column. Sepharose 64-73 immunoglobulin heavy constant alpha 1 Homo sapiens 9-13 15593035-4 2004 A jacalin-agarose column fractionated the IgA. Sepharose 10-17 immunoglobulin heavy variable 4-38-2-like Homo sapiens 42-45 15358369-6 2004 Due to ease in preparing the Sepharose-IDA-Zn(2+) column, and purifying hCA-II just in one step, the overall protocol will be ideal for producing bulk quantities of the enzyme for high throughput screening of inhibitors. Sepharose 29-38 carbonic anhydrase 2 Homo sapiens 72-78 15358377-2 2004 We describe here an efficient and rapid purification procedure for recombinant alpha-Gal A by sequential Concanavalin A (Con A)-Sepharose and immobilized thio-alpha-galactoside (thio-Gal) agarose column chromatography. Sepharose 128-137 galactosidase alpha Homo sapiens 79-90 15358377-2 2004 We describe here an efficient and rapid purification procedure for recombinant alpha-Gal A by sequential Concanavalin A (Con A)-Sepharose and immobilized thio-alpha-galactoside (thio-Gal) agarose column chromatography. Sepharose 188-195 galactosidase alpha Homo sapiens 79-90 15367350-3 2004 Rabbits anti-RI antibody was obtained after immunization and then purified through rProtein A Sepharose Fast Flow chromatography column. Sepharose 94-103 ribonuclease/angiogenin inhibitor 1 Homo sapiens 13-15 15294293-9 2004 The p38alpha protein was purified to near homogeneity using a simple two-step procedure including nickel-chelating Sepharose chromatography followed by anion-exchange chromatography using MonoQ resin. Sepharose 115-124 mitogen-activated protein kinase 14 Homo sapiens 4-12 15540608-1 2004 Peptides selected from the HIV viral protein gp120 bind to a synthetic peptide mimicking sequence 78-89 of the human lymphocyte CD4 molecule, linked to activated Sepharose. Sepharose 162-171 inter-alpha-trypsin inhibitor heavy chain 4 Homo sapiens 45-50 15476317-5 2004 Agarose electrophoresis showed that the cells expressed Musashi-1 mRNA. Sepharose 0-7 musashi RNA binding protein 1 Homo sapiens 56-65 15540608-1 2004 Peptides selected from the HIV viral protein gp120 bind to a synthetic peptide mimicking sequence 78-89 of the human lymphocyte CD4 molecule, linked to activated Sepharose. Sepharose 162-171 CD4 molecule Homo sapiens 128-131 15540608-2 2004 The binding of viral fragments to the CD4 peptide-Sepharose beads was ascertained either by aid of a ninhydrin reagent or by fluorescence microscopy. Sepharose 50-59 CD4 molecule Homo sapiens 38-41 15270712-1 2004 Recombinant cabbage (Brassica oleracea) PLD2 (phospholipase D2) immobilized covalently on CNBr-activated Sepharose expressed low activity (approximately 10%), while that immobilized by binding on to anti-PLD2 IgG-Sepharose was more active (approximately 38%). Sepharose 213-222 phospholipase D alpha 2 Brassica oleracea 46-62 15498245-4 2004 Polymerase chain reaction (PCR) and 2.5% agarose gel electrophoresisi were adopted to detect the polymorphism of functional 30 bp-uVNTR in the promoter region of the X-chromosomal MAOA gene and their frequencies of varied genotypes were estimated. Sepharose 41-48 monoamine oxidase A Homo sapiens 180-184 15262496-8 2004 Like K8.1, Orf51 could bind to agarose-conjugated heparin, implicating this molecule in viral attachment to cells. Sepharose 31-38 keratin 81 Homo sapiens 5-9 15270712-2 2004 Coupling of PLD2 to CNBr-activated Sepharose resulted in significant improvement in storage stability without affecting its thermostability, as compared with the soluble enzyme. Sepharose 35-44 phospholipase D alpha 2 Brassica oleracea 12-16 15328291-1 2004 The peptidoglycan recognition protein, PGRP, known as an intracellular component of neutrophils, has been isolated from camel (Camelus dromedarius) milk by acid precipitation followed by heparin-sepharose affinity chromatography of the supernatant. Sepharose 195-204 LOW QUALITY PROTEIN: peptidoglycan recognition protein 1 Camelus dromedarius 39-43 15215307-3 2004 To find out whether these autophosphorylation sites can be used to identify downstream signaling proteins, synthetic peptides based on an autophosphorylation site in the colony-stimulating factor-1 (CSF-1) receptor were linked to agarose beads and incubated with lysates from macrophages. Sepharose 230-237 colony stimulating factor 1 Homo sapiens 170-197 15215307-3 2004 To find out whether these autophosphorylation sites can be used to identify downstream signaling proteins, synthetic peptides based on an autophosphorylation site in the colony-stimulating factor-1 (CSF-1) receptor were linked to agarose beads and incubated with lysates from macrophages. Sepharose 230-237 colony stimulating factor 1 receptor Homo sapiens 199-214 15321764-3 2004 The primers of MN/CAIX gene were designed for a two-step RT-PCR, and the products examined by 2% agarose gel electrophoresis and identified by sequence analysis. Sepharose 97-104 carbonic anhydrase 9 Homo sapiens 18-22 15178109-4 2004 Sharpnose shark, Rhizoprionodon terraenovae, serum C-reactive protein was purified sequentially over AH-sepharose 4B-PC and sepharose CL-4B columns and used to immunize balb/c mice for generating stocks of polyclonal anti-sera. Sepharose 104-113 C-reactive protein, pentraxin-related Mus musculus 51-69 15257753-7 2004 Recombinant AKAP3 and AKAP4 RII binding domains were synthesized as glutathione S-transferase (GST) fusion proteins immobilized on glutathione-agarose resin and added to CHAPS extracts of human spermatozoa. Sepharose 143-150 A-kinase anchoring protein 3 Homo sapiens 12-17 15249052-5 2004 Following cleavage of GST-hGLP-1-Leu by cyanogen bromide, the recombinant hGLP-1-Leu was released from fusion protein, and purified using QAE Sepharose ion exchange and RP C(18) chromatography. Sepharose 142-151 glucagon like peptide 1 receptor Homo sapiens 74-80 15039285-6 2004 Binding of (125)I-IL-1beta to Sepharose-immobilized fibrinogen also demonstrated a single binding site with an apparent K(d) of 3.5 nM. Sepharose 30-39 interleukin 1 beta Homo sapiens 18-26 15039285-6 2004 Binding of (125)I-IL-1beta to Sepharose-immobilized fibrinogen also demonstrated a single binding site with an apparent K(d) of 3.5 nM. Sepharose 30-39 fibrinogen beta chain Homo sapiens 52-62 15257753-7 2004 Recombinant AKAP3 and AKAP4 RII binding domains were synthesized as glutathione S-transferase (GST) fusion proteins immobilized on glutathione-agarose resin and added to CHAPS extracts of human spermatozoa. Sepharose 143-150 A-kinase anchoring protein 4 Homo sapiens 22-27 15248901-7 2004 We could demonstrate that secretion-deficient as well as secretion-competent YopE-DHFR fusions complexed to SycE can be efficiently purified from Yersinia cytosol by affinity chromatography using methotrexate-agarose. Sepharose 209-216 dihydrofolate reductase Mus musculus 82-86 15248901-7 2004 We could demonstrate that secretion-deficient as well as secretion-competent YopE-DHFR fusions complexed to SycE can be efficiently purified from Yersinia cytosol by affinity chromatography using methotrexate-agarose. Sepharose 209-216 sycE Yersinia enterocolitica 108-112 15228334-1 2004 We have detected single-molecule binding interactions of a target peptide with the calcium-signaling protein calmodulin (CaM) immobilized in an agarose gel, and we have demonstrated the application of a single-molecule binding assay to measure the binding strength of CaM with the CaM-binding domain of calmodulin-dependent protein kinase II (CaMKII). Sepharose 144-151 calmodulin 1 Homo sapiens 109-119 15270712-1 2004 Recombinant cabbage (Brassica oleracea) PLD2 (phospholipase D2) immobilized covalently on CNBr-activated Sepharose expressed low activity (approximately 10%), while that immobilized by binding on to anti-PLD2 IgG-Sepharose was more active (approximately 38%). Sepharose 105-114 phospholipase D alpha 2 Brassica oleracea 40-44 15270712-1 2004 Recombinant cabbage (Brassica oleracea) PLD2 (phospholipase D2) immobilized covalently on CNBr-activated Sepharose expressed low activity (approximately 10%), while that immobilized by binding on to anti-PLD2 IgG-Sepharose was more active (approximately 38%). Sepharose 105-114 phospholipase D alpha 2 Brassica oleracea 46-62 15270712-1 2004 Recombinant cabbage (Brassica oleracea) PLD2 (phospholipase D2) immobilized covalently on CNBr-activated Sepharose expressed low activity (approximately 10%), while that immobilized by binding on to anti-PLD2 IgG-Sepharose was more active (approximately 38%). Sepharose 213-222 phospholipase D alpha 2 Brassica oleracea 40-44 15487703-2 2004 Pre-beta-HDL with a pre-beta mobility on agarose gels is an apoA-I (MW 28 kDa)-rich and a lipid-poor lipoprotein. Sepharose 41-48 apolipoprotein A1 Homo sapiens 60-66 15080792-3 2004 In the present paper, we show that ErbB2 could be pulled-down using CaM-agarose beads in a Ca2+-dependent manner, as detected by Western blot analysis using an anti-ErbB2 antibody. Sepharose 72-79 erb-b2 receptor tyrosine kinase 2 Homo sapiens 35-40 15080792-3 2004 In the present paper, we show that ErbB2 could be pulled-down using CaM-agarose beads in a Ca2+-dependent manner, as detected by Western blot analysis using an anti-ErbB2 antibody. Sepharose 72-79 calmodulin 3 Homo sapiens 68-71 15080792-3 2004 In the present paper, we show that ErbB2 could be pulled-down using CaM-agarose beads in a Ca2+-dependent manner, as detected by Western blot analysis using an anti-ErbB2 antibody. Sepharose 72-79 erb-b2 receptor tyrosine kinase 2 Homo sapiens 165-170 15228334-1 2004 We have detected single-molecule binding interactions of a target peptide with the calcium-signaling protein calmodulin (CaM) immobilized in an agarose gel, and we have demonstrated the application of a single-molecule binding assay to measure the binding strength of CaM with the CaM-binding domain of calmodulin-dependent protein kinase II (CaMKII). Sepharose 144-151 calmodulin 1 Homo sapiens 121-124 15228334-5 2004 As an alternative to surface immobilization, we have constructed a CaM/maltose binding protein fusion protein, which renders CaM translationally immobile in a low weight percent agarose gel. Sepharose 178-185 calmodulin 1 Homo sapiens 67-70 15228334-5 2004 As an alternative to surface immobilization, we have constructed a CaM/maltose binding protein fusion protein, which renders CaM translationally immobile in a low weight percent agarose gel. Sepharose 178-185 calmodulin 1 Homo sapiens 125-128 15228334-6 2004 The target binding functionality of CaM assayed in agarose gels is in good agreement with solution assays. Sepharose 51-58 calmodulin 1 Homo sapiens 36-39 15183422-4 2004 Addition of Affi-gel agarose beads loaded with TGF-beta1 (10 ng/microl) to developing stage 24-25 chick limb buds resulted in limb shortening and altered morphology. Sepharose 21-28 transforming growth factor beta 1 Gallus gallus 47-56 15497503-6 2004 Using a binding protein-retention assay with CyP40 fused to glutathione S-transferase immobilized on glutathione-agarose, we have identified the constitutively expressed form of Hsp70, heat shock cognate (Hsc)70, as an additional target for CyP40. Sepharose 113-120 heat shock protein family A (Hsp70) member 4 Homo sapiens 178-183 15059947-5 2004 The T(4) effect was apparent at 15 min and peaked at 2 h, whereas the E(2) effect was maximal at 4-6 h. T(4)-agarose was as effective as T(4) in causing phosphorylation of ERalpha. Sepharose 109-116 estrogen receptor 1 Homo sapiens 172-179 15075362-9 2004 In the chemotaxis assay under agarose, MCP-3 dose-dependently increased the migration distance of neutrophils toward IL-8. Sepharose 30-37 C-C motif chemokine ligand 7 Homo sapiens 39-44 15238456-3 2004 METHODS AND RESULTS: SDS-agarose gels revealed small N2B (stiff) and large N2BA (compliant) cardiac titin isoforms with a mean N2BA:N2B expression ratio that was significantly (P<0.003) increased in 20 heart failure patients versus 6 controls. Sepharose 25-32 titin Homo sapiens 100-105 15191539-6 2004 MSP was incubated with agarose beads and injected subcutaneously into mice all 70 d old when a uniform telogen state in dorsal skin was apparent. Sepharose 23-30 macrophage stimulating 1 (hepatocyte growth factor-like) Mus musculus 0-3 15177281-4 2004 This chimeric protein was purified by affinity chromatography on calmodulin-Sepharose with mild elution with EDTA. Sepharose 76-85 calmodulin 1 Homo sapiens 65-75 15082230-6 2004 All these molecules bound to fasciculin-2-Sepharose, although the extent of binding was higher for G1H (77%) than for G1A (63%) or G2A (48%) forms. Sepharose 42-51 G protein-coupled receptor 132 Mus musculus 131-134 15207081-6 2004 Expression of hIL-21 was induced by IPTG at 37 degrees Celsius for 5 h. The target protein was purified through Ni(2+)-chelating Sepharose Fast Flow. Sepharose 129-138 interleukin 21 Homo sapiens 14-20 15108362-10 2004 The association of TNF-alpha with heparin was shown by demonstrating that TNF-alpha bound tightly to heparin-Sepharose chromatographic material. Sepharose 109-118 tumor necrosis factor Homo sapiens 19-28 15478465-4 2004 SAP was purified from serum by affinity chromatography using phosphorylethanolamine-coupled ECH-sepharose 4B. Sepharose 96-108 amyloid P component, serum Homo sapiens 0-3 15198879-4 2004 Multimers of vWF in plasma of patients with TTP were also analyzed by SDS-agarose electrophoresis. Sepharose 74-81 von Willebrand factor Homo sapiens 13-16 15073173-7 2004 From extracts of rat brain and rat primary cultured neurons, Ser(9)-phosphorylated GSK3beta precipitates with glutathione-agarose beads coated with glutathione S-transferase-14-3-3. Sepharose 122-129 glycogen synthase kinase 3 beta Rattus norvegicus 83-91 15182177-5 2004 In this test, Grb2 is pulled down from a cellular extract by the initial VPPPVPPRRR peptide bound to Sepharose beads. Sepharose 101-110 growth factor receptor bound protein 2 Homo sapiens 14-18 15110774-3 2004 To search for such proteins, we used a Sepharose-conjugated peptide containing phosphotyrosine 220 (PTyr-220) of Dab1, as an affinity matrix to capture binding proteins from mouse brain extracts. Sepharose 39-48 disabled 1 Mus musculus 113-117 15108362-10 2004 The association of TNF-alpha with heparin was shown by demonstrating that TNF-alpha bound tightly to heparin-Sepharose chromatographic material. Sepharose 109-118 tumor necrosis factor Homo sapiens 74-83 15177873-3 2004 The GST-hTFF3 fusion protein was expressed in Escherichia coli, and hTFF3 was purified with Glutathione Sepharose 4B affinity chromatography, yielding about 3-4 mg of pure hTFF3 in one liter of culture broth. Sepharose 104-113 glutathione S-transferase kappa 1 Homo sapiens 4-7 15081409-4 2004 Binding evaluations have primarily relied on IQGAP1 interaction with calmodulin conjugated to Sepharose 4B. Sepharose 94-106 IQ motif containing GTPase activating protein 1 Homo sapiens 45-51 15081409-4 2004 Binding evaluations have primarily relied on IQGAP1 interaction with calmodulin conjugated to Sepharose 4B. Sepharose 94-106 calmodulin 1 Homo sapiens 69-79 15081900-8 2004 Recombinant PARG was immobilized using an affinity column composed of tannins covalently linked to Sepharose beads. Sepharose 99-108 poly(ADP-ribose) glycohydrolase Homo sapiens 12-16 15971615-18 2004 Finally, simple purification procedure based on immobilized metal affinity column (IMAC) and CM-Sepharose column was implemented to isolate the TRAIL. Sepharose 96-105 TNF superfamily member 10 Homo sapiens 144-149 15177873-3 2004 The GST-hTFF3 fusion protein was expressed in Escherichia coli, and hTFF3 was purified with Glutathione Sepharose 4B affinity chromatography, yielding about 3-4 mg of pure hTFF3 in one liter of culture broth. Sepharose 104-113 trefoil factor 3 Homo sapiens 68-73 15177873-3 2004 The GST-hTFF3 fusion protein was expressed in Escherichia coli, and hTFF3 was purified with Glutathione Sepharose 4B affinity chromatography, yielding about 3-4 mg of pure hTFF3 in one liter of culture broth. Sepharose 104-113 trefoil factor 3 Homo sapiens 68-73 15072287-2 2004 A variety of molecules were screened as possible displacers for the proteins ribonuclease A (RNAseA) and alpha-chymotrypsinogen A (alpha-chyA) on high performance Sepharose SP. Sepharose 163-172 ribonuclease A family member 1, pancreatic Homo sapiens 77-91 15072287-2 2004 A variety of molecules were screened as possible displacers for the proteins ribonuclease A (RNAseA) and alpha-chymotrypsinogen A (alpha-chyA) on high performance Sepharose SP. Sepharose 163-172 ribonuclease A family member 1, pancreatic Homo sapiens 93-99 14766798-4 2004 Expression of a versican G3 construct in U87 astrocytoma cells enhances colony growth in soft agarose gel and tumor growth and blood vessel formation in nude mice. Sepharose 94-101 versican Mus musculus 16-24 15047532-4 2004 EcoRI and BamHI digests of qnr-hybridizing plasmids were subjected to electrophoresis on agarose gels and probed with qnr by Southern hybridization. Sepharose 89-96 Qnr Escherichia coli 27-30 14769331-5 2004 Pre-treatment of either lambda DNA or the promoter region of human telomerase (hTERT) with HaeIII methylase greatly reduced the digestion of the DNAs with the corresponding restriction enzyme HaeIII endonuclease (over 100-fold), and the result was further confirmed by agarose gel electrophoresis. Sepharose 269-276 telomerase reverse transcriptase Homo sapiens 79-84 14999746-4 2004 Osteopontin purified from bovine milk was either absorbed to agarose beads or crosslinked to agarose beads. Sepharose 61-68 secreted phosphoprotein 1 Bos taurus 0-11 14999746-4 2004 Osteopontin purified from bovine milk was either absorbed to agarose beads or crosslinked to agarose beads. Sepharose 93-100 secreted phosphoprotein 1 Bos taurus 0-11 14999746-6 2004 Apatite was induced by osteopontin that was crosslinked to agarose beads, whereas osteopontin adsorbed to agarose beads failed to induce apatite crystal formation. Sepharose 106-113 secreted phosphoprotein 1 Bos taurus 82-93 14999746-7 2004 Using classical nucleation theory, the interfacial energy for hydroxyapatite nucleation on osteopontin crosslinked to agarose beads was determined to be 94.7 ergs/cm(2). Sepharose 118-125 secreted phosphoprotein 1 Bos taurus 91-102 15003259-0 2004 Purification of human erythrocyte glucose 6-phosphate dehydrogenase and glutathione reductase enzymes using 2",5"-ADP Sepharose 4B affinity column material in single chromatographic step. Sepharose 118-127 glucose-6-phosphate dehydrogenase Homo sapiens 34-67 15003259-0 2004 Purification of human erythrocyte glucose 6-phosphate dehydrogenase and glutathione reductase enzymes using 2",5"-ADP Sepharose 4B affinity column material in single chromatographic step. Sepharose 118-127 glutathione-disulfide reductase Homo sapiens 72-93 15003527-5 2004 The recombinant human 6x His-PC isolated with a purity of approximately 50% using a Ni-NTA agarose column was found to have the specific activity of 7U/mg, which was similar to that produced from a 293T stable line [Biochem. Sepharose 91-98 pyruvate carboxylase Homo sapiens 29-31 15033618-6 2004 Angiotensin converting enzyme genotypes were determined by agarose gel sizing after polymerase chain reaction (PCR) amplification. Sepharose 59-66 angiotensin I converting enzyme Homo sapiens 0-29 14659430-3 2004 For this purpose, the highly selective PTP1B inhibitor 2-(oxalyl-amino)-4,5,6,7-tetrahydro-thieno[2,3-c]pyridine-3-carboxylic acid (OTP) was coupled to epoxy-activated Sepharose 6B (OTP Sepharose) and used for one-step affinity purification of tag-free PTP1B. Sepharose 168-177 protein tyrosine phosphatase non-receptor type 1 Homo sapiens 39-44 15161023-5 2004 Agarose gel electrophoresis showed that HK1 induced internucleosomal DNA fragmentation in HL-60 cells, but the addition of FeCl3 inhibited the DNA fragmentation. Sepharose 0-7 hexokinase 1 Homo sapiens 40-43 15036398-2 2004 The present study determined if aging affects the ability of interleukin-1beta (IL-1beta), a pro-inflammatory cytokine, to induce increased NO production (assessed by Griess reaction) and iNOS mRNA levels (assessed by RT-PCR/agarose gel electrophoresis) in vascular smooth muscle cells (VSMCs) from young (3-month-old) and elderly (20-22-month-old) rats. Sepharose 225-232 interleukin 1 beta Rattus norvegicus 61-78 15036398-2 2004 The present study determined if aging affects the ability of interleukin-1beta (IL-1beta), a pro-inflammatory cytokine, to induce increased NO production (assessed by Griess reaction) and iNOS mRNA levels (assessed by RT-PCR/agarose gel electrophoresis) in vascular smooth muscle cells (VSMCs) from young (3-month-old) and elderly (20-22-month-old) rats. Sepharose 225-232 interleukin 1 beta Rattus norvegicus 80-88 14977953-4 2004 Wild-type mice fed the liquid diet used to prevent intestinal obstruction in Cftr-knockout mice had inflammatory responses to P. aeruginosa-laden agarose beads similar to those of wild-type mice fed an enriched solid diet, so dietary effects are unlikely to account for differences between wild-type mice and mice with cystic fibrosis. Sepharose 146-153 cystic fibrosis transmembrane conductance regulator Mus musculus 77-81 14724321-6 2004 The putative RNA binding protein ZAP was also established as a nuclear PP1 binding protein using the criteria of co-purification with PP1 on microcystin-Sepharose, co-immunoprecipation, binding PP1 in an overlay assay, and presence of a putative PP1 binding site (KKRVRWAD). Sepharose 153-162 zinc finger CCCH-type containing, antiviral 1 Homo sapiens 33-36 14724321-6 2004 The putative RNA binding protein ZAP was also established as a nuclear PP1 binding protein using the criteria of co-purification with PP1 on microcystin-Sepharose, co-immunoprecipation, binding PP1 in an overlay assay, and presence of a putative PP1 binding site (KKRVRWAD). Sepharose 153-162 inorganic pyrophosphatase 1 Homo sapiens 71-74 14724321-6 2004 The putative RNA binding protein ZAP was also established as a nuclear PP1 binding protein using the criteria of co-purification with PP1 on microcystin-Sepharose, co-immunoprecipation, binding PP1 in an overlay assay, and presence of a putative PP1 binding site (KKRVRWAD). Sepharose 153-162 inorganic pyrophosphatase 1 Homo sapiens 134-137 14724321-6 2004 The putative RNA binding protein ZAP was also established as a nuclear PP1 binding protein using the criteria of co-purification with PP1 on microcystin-Sepharose, co-immunoprecipation, binding PP1 in an overlay assay, and presence of a putative PP1 binding site (KKRVRWAD). Sepharose 153-162 inorganic pyrophosphatase 1 Homo sapiens 134-137 14724321-6 2004 The putative RNA binding protein ZAP was also established as a nuclear PP1 binding protein using the criteria of co-purification with PP1 on microcystin-Sepharose, co-immunoprecipation, binding PP1 in an overlay assay, and presence of a putative PP1 binding site (KKRVRWAD). Sepharose 153-162 inorganic pyrophosphatase 1 Homo sapiens 134-137 15030172-5 2004 Heparin sepharose purified preparation of 72 kDa progelatinase is composed of two distinct population of zymogens: a 72 kDa progelatinase tightly complexed with TIMP-2 (an ambient tissue inhibitor of metalloprotease in the smooth muscle plasma membrane), and a native 72 kDa progelatinase free of any detectable TIMP-2. Sepharose 8-17 TIMP metallopeptidase inhibitor 2 Bos taurus 161-167 15030172-5 2004 Heparin sepharose purified preparation of 72 kDa progelatinase is composed of two distinct population of zymogens: a 72 kDa progelatinase tightly complexed with TIMP-2 (an ambient tissue inhibitor of metalloprotease in the smooth muscle plasma membrane), and a native 72 kDa progelatinase free of any detectable TIMP-2. Sepharose 8-17 TIMP metallopeptidase inhibitor 2 Bos taurus 312-318 14984739-3 2004 ZAG was purified from human plasma using a combination of Q Sepharose and Superdex 75 chromatography, and was shown to stimulate glycerol release from isolated murine epididymal adipocytes in a dose-dependent manner. Sepharose 60-69 alpha-2-glycoprotein 1, zinc-binding Homo sapiens 0-3 14978261-3 2004 Using two-dimensional agarose gels, chemical probing and atomic force microscopy, we characterized the formation of non-B-DNA structures in the Friedreich ataxia-associated (GAA)n*(TTC)n repeats from the FRDA gene that were cloned with flanking genomic sequences into plasmids. Sepharose 22-29 alpha glucosidase Homo sapiens 174-177 14751262-3 2004 The small size of CaM hinders its immobilization in low-weight-percentage agarose gels; however, fusion of CaM to MBP via a flexible linker provides sufficient restriction of translational mobility in 1% agarose gels. Sepharose 204-211 calmodulin 1 Homo sapiens 107-110 14751262-3 2004 The small size of CaM hinders its immobilization in low-weight-percentage agarose gels; however, fusion of CaM to MBP via a flexible linker provides sufficient restriction of translational mobility in 1% agarose gels. Sepharose 204-211 myelin basic protein Homo sapiens 114-117 14594800-5 2004 Fas binds to CaM-Sepharose and is co-immunoprecipitated with CaM. Sepharose 17-26 calmodulin 2 Mus musculus 13-16 14960307-2 2004 The assay of adsorption from ER extract with glutathione S-transferase-mFKBP23 attached to glutathione-Sepharose 4B shows that mFKBP23 binds to mouse immunoglobulin binding protein (mBiP). Sepharose 103-112 FK506 binding protein 7 Mus musculus 71-78 14960307-2 2004 The assay of adsorption from ER extract with glutathione S-transferase-mFKBP23 attached to glutathione-Sepharose 4B shows that mFKBP23 binds to mouse immunoglobulin binding protein (mBiP). Sepharose 103-112 FK506 binding protein 7 Mus musculus 127-134 14960307-2 2004 The assay of adsorption from ER extract with glutathione S-transferase-mFKBP23 attached to glutathione-Sepharose 4B shows that mFKBP23 binds to mouse immunoglobulin binding protein (mBiP). Sepharose 103-112 heat shock protein 5 Mus musculus 182-186 14744997-4 2004 In living mice, mesoangioblasts injected into the femoral artery migrate close to HMGB1-loaded heparin-Sepharose beads implanted in healthy muscle, but are unresponsive to control beads. Sepharose 103-112 high mobility group box 1 Mus musculus 82-87 14984498-1 2004 Internal tandem duplications in FLT3 are the most common mutation in acute myeloid leukaemia (AML), with agarose gel electrophoresis of polymerase chain reaction products (PCR/agarose) being the screening method of choice for these mutations. Sepharose 105-112 fms related receptor tyrosine kinase 3 Homo sapiens 32-36 14984498-1 2004 Internal tandem duplications in FLT3 are the most common mutation in acute myeloid leukaemia (AML), with agarose gel electrophoresis of polymerase chain reaction products (PCR/agarose) being the screening method of choice for these mutations. Sepharose 176-183 fms related receptor tyrosine kinase 3 Homo sapiens 32-36 14734213-3 2004 We studied the frequencies of the apoE alleles and genotypes in the three ethnic groups-Malay, Chinese and Indian-in Malaysia using DNA amplification followed by agarose gel electrophoresis. Sepharose 162-169 apolipoprotein E Homo sapiens 34-38 18432922-4 2004 Also described is a procedure for preparing GSThyphen;cJun/GSH-Sepharose beads needed in the solid-phase JNK protein kinase activity assay. Sepharose 63-72 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 54-58 18432922-4 2004 Also described is a procedure for preparing GSThyphen;cJun/GSH-Sepharose beads needed in the solid-phase JNK protein kinase activity assay. Sepharose 63-72 mitogen-activated protein kinase 8 Homo sapiens 105-108 14744877-7 2004 EBP50 and ezrin in solubilized microsomes bound to CRALBP-agarose but not to a control agarose column. Sepharose 58-65 solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 Mus musculus 0-5 14744877-7 2004 EBP50 and ezrin in solubilized microsomes bound to CRALBP-agarose but not to a control agarose column. Sepharose 58-65 ezrin Mus musculus 10-15 14744877-7 2004 EBP50 and ezrin in solubilized microsomes bound to CRALBP-agarose but not to a control agarose column. Sepharose 58-65 retinaldehyde binding protein 1 Mus musculus 51-57 14705189-8 2004 Moreover, the utilization of histidine-immobilized agarose gel effectively concentrated the trace amount of LPS from the C(12)E(10)-solubilized HbV solution and washed out C(12)E(10) as an inhibitory element. Sepharose 51-58 interferon regulatory factor 6 Homo sapiens 108-111 14760758-2 2004 METHODS: By ConA-sepharose affinity chromatography, ADP-agarose affinity chromatography, and DEAE anion exchange chromatography, we were able to purify HSP70-associated peptides from mouse hepatoma (HCaF) cells treated in heat shock at 42 degrees. Sepharose 17-26 heat shock protein 1B Mus musculus 152-157 14753708-2 2004 When lac-repressor-beta-galactosidase fusion protein is loaded onto a new DNA-Sepharose column, less elutes from a new column than one that has been used two or more times. Sepharose 78-87 galactosidase beta 1 Homo sapiens 19-37 14711380-7 2004 Duplication time, plating efficiency, colony formation in agarose, and contact inhibition were also altered in the p53 mutated tumor cultures compared to those carrying wild-type p53. Sepharose 58-65 tumor protein p53 Homo sapiens 115-118 14659430-5 2004 Importantly, since OTP Sepharose binds PTP1B with an intact active site only, the method ensures that the purified enzyme is fully active, a feature that might be particularly important in PTP research. Sepharose 23-32 protein tyrosine phosphatase non-receptor type 1 Homo sapiens 39-44 15493579-7 2004 Amplification of C-MYC and C-ERBB2 genes was evaluated by densitometry after agarose gel separation of the respective multiplex PCR products. Sepharose 77-84 MYC proto-oncogene, bHLH transcription factor Homo sapiens 17-22 15081544-1 2004 The variable fragment (Fv) of the monoclonal B1-8 antibody recognizes 3-nitro-4-hydroxy-phenylacetate (NP) and 5-iodo-NP (NIP) allowing for the affinity purification of the respective B cell antigen receptor with NP-sepharose and its specific elution with NIP-capronic acid (NIPcap). Sepharose 216-225 UDP glucuronosyltransferase family 1 member A1 Rattus norvegicus 45-49 15493579-7 2004 Amplification of C-MYC and C-ERBB2 genes was evaluated by densitometry after agarose gel separation of the respective multiplex PCR products. Sepharose 77-84 erb-b2 receptor tyrosine kinase 2 Homo sapiens 27-34 14675065-6 2004 Soluble scFv was expressed by a yeast secretion vector, purified, and immobilized onto agarose beads. Sepharose 87-94 immunglobulin heavy chain variable region Homo sapiens 8-12 15103077-4 2004 ACF was purified using synthetic wild-type and mutant apo-B RNAs, which were coupled to cyanogen bromide (CNBr)- activated Sepharose. Sepharose 123-132 APOBEC1 complementation factor Homo sapiens 0-3 14962105-7 2004 MT1-MMP expression results in enhanced 3 dimensional growth in agarose gels and in long-term cultures within matrigel. Sepharose 63-70 matrix metallopeptidase 14 Homo sapiens 0-7 15103077-4 2004 ACF was purified using synthetic wild-type and mutant apo-B RNAs, which were coupled to cyanogen bromide (CNBr)- activated Sepharose. Sepharose 123-132 apolipoprotein B Homo sapiens 54-59 14691567-8 2004 Sepharose beads covalently coated with either whole fibrinogen or Haptides (SB-Fib or SB-Haptide) highly adsorbed free (FITC) Haptides. Sepharose 0-9 fibrinogen beta chain Homo sapiens 52-62 15253024-5 2004 Plasmatic FN was assessed by radial immunodiffusion with anti-human FN in 1% agarose gel slabs. Sepharose 77-84 fibronectin 1 Homo sapiens 10-12 14644171-6 2003 In contrast, both of these integrins bound to fibronectin-Sepharose. Sepharose 58-67 fibronectin 1 Homo sapiens 46-57 14735978-3 2003 An experimental study on the interplay of sorbent structure and fluid phase conditions (pH) has been carried out examining adsorption and transport of bovine serum albumin (BSA) and a monoclonal antibody (IgG 2a) on SP Sepharose Fast Flow and SP Sepharose XL. Sepharose 219-228 albumin Homo sapiens 158-171 14506265-2 2003 Although we have previously observed that myristoylated and non-myristoylated MARCKS proteins behave differently during calmodulin-agarose chromatography, the role of protein myristoylation in the MARCKS-calmodulin interaction remained to be elucidated. Sepharose 131-138 myristoylated alanine rich protein kinase C substrate Homo sapiens 78-84 14506265-2 2003 Although we have previously observed that myristoylated and non-myristoylated MARCKS proteins behave differently during calmodulin-agarose chromatography, the role of protein myristoylation in the MARCKS-calmodulin interaction remained to be elucidated. Sepharose 131-138 calmodulin 1 Homo sapiens 120-130 14506265-4 2003 Both myristoylated and non-myristoylated recombinant MARCKS bound to calmodulin-agarose at low ionic strengths, but only the former retained the affinity at high ionic strengths. Sepharose 80-87 myristoylated alanine rich protein kinase C substrate Homo sapiens 53-59 14506265-4 2003 Both myristoylated and non-myristoylated recombinant MARCKS bound to calmodulin-agarose at low ionic strengths, but only the former retained the affinity at high ionic strengths. Sepharose 80-87 calmodulin 1 Homo sapiens 69-79 14719801-1 2003 Haptoglobin (Hp) can be purified by affinity chromatography using hemoglobin (Hb)-linked Sepharose. Sepharose 89-98 haptoglobin Homo sapiens 0-11 14697747-5 2003 The CCR5-Delta32 mutation was identified by PCR amplification and subsequent agarose gel electrophoresis. Sepharose 77-84 C-C motif chemokine receptor 5 Homo sapiens 4-8 14633848-5 2003 Human FN3K-RP was transfected in human embryonic kidney (HEK) cells, and the expressed protein was partially purified by chromatography on Blue Sepharose. Sepharose 144-153 fructosamine 3 kinase related protein Homo sapiens 6-13 14662312-8 2003 In agarose gel electrophoresis, plasma apoE was found in beta-migrating lipoprotein, but it was not present in alpha-migrating lipoprotein. Sepharose 3-10 apolipoprotein E Bos taurus 39-43 14662947-1 2003 Hepatitis B virus X protein (HBx) expressed in Escherichia coli DH5alpha by recombinant DNA technology was purified to homogeneity by use of glutathione-Sepharose beads. Sepharose 153-162 X protein Hepatitis B virus 29-32 14624766-4 2003 In the presence of IGF-II, IGFBP-2 bound with high affinity to heparin-Sepharose, but not to type I collagen, fibronectin, or laminin. Sepharose 71-80 insulin like growth factor 2 Homo sapiens 19-25 14624766-4 2003 In the presence of IGF-II, IGFBP-2 bound with high affinity to heparin-Sepharose, but not to type I collagen, fibronectin, or laminin. Sepharose 71-80 insulin like growth factor binding protein 2 Homo sapiens 27-34 14568190-0 2003 Tamoxifen might influence the affinity of LPL for heparin-sepharose. Sepharose 58-67 lipoprotein lipase Homo sapiens 42-45 14555324-3 2003 DNA extracted from mouse SVs 2 days after castration on days 0, 5, 10, and 60 showed a ladder pattern on agarose gel electrophoresis. Sepharose 105-112 semenogelin 1 Mus musculus 25-30 14622109-6 2003 As the tissue concentration of brain Cln3p was much lower than that of liver Cln3p, it could be detected only after purification from brain extract using anti-Cln3p IgG Sepharose. Sepharose 169-178 ceroid lipofuscinosis, neuronal 3, juvenile (Batten, Spielmeyer-Vogt disease) Mus musculus 37-42 14647463-2 2003 To identify a novel protein(s) binding to PU.1, we carried out affinity purification using a column of Glutathione-Sepharose beads bound to GST-PU.1 fusion protein and isolated several individual proteins using murine erythroleukemia (MEL) cell extracts. Sepharose 115-124 spleen focus forming virus (SFFV) proviral integration oncogene Mus musculus 42-46 12946270-9 2003 PON3 has been purified 177-fold to apparent homogeneity with a final specific activity of 461 units/mg using a method consisting of seven steps: solubilization of the microsomal fraction, hydroxyapatite adsorption, chromatography on DEAE-Sepharose CL-6B, non-specific affinity chromatography on Cibacron Blue 3GA, two DEAE-cellulose steps and a final affinity chromatography on concanavalin A-Sepharose. Sepharose 238-247 paraoxonase 3 Rattus norvegicus 0-4 14573702-4 2003 The technique relies on the detection of an agarose bead detachment from the tip of a micromachined cantilever resulting from BoNT-B action on its substratum, the synaptic protein synaptobrevin 2, attached to the beads. Sepharose 44-51 vesicle associated membrane protein 2 Homo sapiens 180-195 14568190-3 2003 METHODS: Lipoprotein lipase in post-heparin plasma usually exists in both monomeric and dimeric forms, which may be separated on a heparin-Sepharose column with different salt concentrations. Sepharose 139-148 lipoprotein lipase Homo sapiens 9-27 14568190-4 2003 Lipoprotein lipase in post-heparin plasma from postmenopausal patients with hypertriglyceridemia treated with or without tamoxifen was incubated with or without 4-hydroxy-tamoxifen (4-OHT), the monomers and dimers were separated on a heparin-Sepharose column and their masses were measured. Sepharose 242-251 lipoprotein lipase Homo sapiens 0-18 14568190-6 2003 Monomeric LPL of tamoxifen-treated patients passed more slowly through the heparin-Sepharose column compared with that of control subjects. Sepharose 83-92 lipoprotein lipase Homo sapiens 10-13 14568190-8 2003 In addition, monomeric LPL incubated with 4-OHT passed more slowly through the heparin-Sepharose column compared with that incubated without 4-OHT. Sepharose 87-96 lipoprotein lipase Homo sapiens 23-26 12890668-5 2003 The DNA binding motif of JMJ was determined using CASTing experiments by incubating a random oligonucleotide library with the GST-JMJ fusion protein coupled to agarose beads. Sepharose 160-167 jumonji, AT rich interactive domain 2 Mus musculus 25-28 14606683-1 2003 Using a combination of Cohn ethanol fractionation, virus inactivation, glycine and sodium chloride precipitation, and lysine-Sepharose affinity chromatography, a unique and rapid simplified method was developed to obtain highly purified fibrinogen for diagnostic use with both biological (Clauss method) and immunological (Jacobsson method) activity. Sepharose 125-134 fibrinogen beta chain Homo sapiens 237-247 14614543-1 2003 To find an effective and quick way of purifying and identifying recombinant human IFN-beta (rhIFN-beta) expressed in yeast Pichia pastoris, Blue Sepharose 6 fast flow (Blue S6FF) and immunological affinity chromatography (IAC) were compared in this report. Sepharose 145-154 interferon beta 1 Homo sapiens 82-90 14534780-8 2003 Semi-purified circulating VEGF proteins were obtained by heparin-sepharose and its biological activities were shown to alter gene expressions in human aortic endothelial cells. Sepharose 65-74 vascular endothelial growth factor A Homo sapiens 26-30 14622300-4 2003 The lectin was purified by affinity chromatography using bovine submaxillary mucin-coupled agarose. Sepharose 91-98 mucin 1, cell surface associated Bos taurus 77-82 12890668-5 2003 The DNA binding motif of JMJ was determined using CASTing experiments by incubating a random oligonucleotide library with the GST-JMJ fusion protein coupled to agarose beads. Sepharose 160-167 jumonji, AT rich interactive domain 2 Mus musculus 130-133 12885773-3 2003 Laminin-Sepharose and syntrophin-Sepharose bind a protein complex containing Rac1 from the muscle membranes. Sepharose 8-17 ras-related C3 botulinum toxin substrate 1 Oryctolagus cuniculus 77-81 14583450-9 2003 Coimmunoprecipitation assays using agarose-conjugated goat antihuman RRM1 antibody confirmed that the p53R2 binding to hRRM1 decreased in PC3 cells but increased in KB cells after UV treatment. Sepharose 35-42 ribonucleotide reductase catalytic subunit M1 Homo sapiens 69-73 14583450-9 2003 Coimmunoprecipitation assays using agarose-conjugated goat antihuman RRM1 antibody confirmed that the p53R2 binding to hRRM1 decreased in PC3 cells but increased in KB cells after UV treatment. Sepharose 35-42 ribonucleotide reductase regulatory TP53 inducible subunit M2B Homo sapiens 102-107 14583450-9 2003 Coimmunoprecipitation assays using agarose-conjugated goat antihuman RRM1 antibody confirmed that the p53R2 binding to hRRM1 decreased in PC3 cells but increased in KB cells after UV treatment. Sepharose 35-42 ribonucleotide reductase catalytic subunit M1 Homo sapiens 119-124 12885773-3 2003 Laminin-Sepharose and syntrophin-Sepharose bind a protein complex containing Rac1 from the muscle membranes. Sepharose 33-42 ras-related C3 botulinum toxin substrate 1 Oryctolagus cuniculus 77-81 12960244-3 2003 First, we examined binding of MPO to CS-Sepharose and measured an ionic interaction, which was disrupted by 200-400 mM NaCl. Sepharose 40-49 myeloperoxidase Homo sapiens 30-33 16281562-2 2003 According to Agarose Diffusion Assay, this recombinant Cecropin-XJ has exhibited an extreme heat-stable characteristic and the ability to kill ampicillin-resistant S. aureus and S. enterica. Sepharose 13-20 cecropin-B Bombyx mori 55-66 12737627-5 2003 Using a microcystin-Sepharose affinity column we purified the native T. cruzi PP2A. Sepharose 20-29 protein phosphatase 2 phosphatase activator Homo sapiens 78-82 14517075-6 2003 Analysis of virion-derived RNA in native agarose gels shows that deletion of SL3 leads to decreases in both viral RNA packaging and dimerization. Sepharose 41-48 matrix metallopeptidase 11 Homo sapiens 77-80 12890752-7 2003 Cells lacking myosin II heavy chain (mhcA-) are unable to migrate under agarose overlays of greater than 0.5%, and even at this concentration they move only a short distance from the trough. Sepharose 72-79 myosin heavy chain 14 Homo sapiens 14-20 12890752-10 2003 Consistent with a role for myosin II in general cortical stability, GFP-myosin dynamically localizes to the lateral and posterior cortex of cells moving under agarose. Sepharose 159-166 myosin heavy chain 14 Homo sapiens 72-78 12890752-12 2003 Mutants lacking either ABP-120 or alpha-actinin were also able to move under agarose at rates similar to wild-type cells. Sepharose 77-84 actinin alpha 1 Homo sapiens 34-47 12935804-1 2003 A manufacturing process for the production of Anti-thrombin IIII concentrate is described, which is based primarily on Heparin Sepharose affinity chromatography. Sepharose 127-136 coagulation factor II, thrombin Homo sapiens 51-59 12821674-6 2003 Direct physical interaction of PLC beta 3 and PLC beta 1 isoforms with CaM is supported by pull-down of both isoenzymes with CaM-Sepharose beads from 1321N1 cell lysates. Sepharose 129-138 phospholipase C beta 3 Homo sapiens 31-41 12821674-6 2003 Direct physical interaction of PLC beta 3 and PLC beta 1 isoforms with CaM is supported by pull-down of both isoenzymes with CaM-Sepharose beads from 1321N1 cell lysates. Sepharose 129-138 phospholipase C beta 1 Homo sapiens 46-56 12821674-6 2003 Direct physical interaction of PLC beta 3 and PLC beta 1 isoforms with CaM is supported by pull-down of both isoenzymes with CaM-Sepharose beads from 1321N1 cell lysates. Sepharose 129-138 calmodulin 3 Homo sapiens 71-74 12821674-6 2003 Direct physical interaction of PLC beta 3 and PLC beta 1 isoforms with CaM is supported by pull-down of both isoenzymes with CaM-Sepharose beads from 1321N1 cell lysates. Sepharose 129-138 calmodulin 3 Homo sapiens 125-128 12724130-4 2003 Both receptors were captured from solubilized renal brush-border membranes by affinity chromatography using myoglobin-Sepharose. Sepharose 118-127 myoglobin Mus musculus 108-117 12938153-6 2003 Reductions of PAI-1 mRNA expression correlated with decreased PAI-1 antigen secretion and PAI-1 activity as assessed by fibrin-agarose zymography. Sepharose 127-134 serpin family E member 1 Homo sapiens 14-19 12788947-8 2003 The peptide was also found to specifically interact with thrombin-agarose. Sepharose 66-73 coagulation factor II, thrombin Homo sapiens 57-65 12810820-6 2003 The HepG2 PLTP could be enriched by Heparin-Sepharose affinity chromatography and eluted in size-exclusion chromatography at a position corresponding to the size of 160 kDa. Sepharose 44-53 phospholipid transfer protein Homo sapiens 10-14 12954240-0 2003 Dynamic compression counteracts IL-1 beta-induced release of nitric oxide and PGE2 by superficial zone chondrocytes cultured in agarose constructs. Sepharose 128-135 interleukin 1 beta Homo sapiens 32-41 12954240-1 2003 OBJECTIVE: To examine the effect of IL-1 beta-induced *NO and PGE(2)release by stimulated superficial and deep chondrocyte/agarose constructs subjected to mechanical compression. Sepharose 123-130 interleukin 1 beta Homo sapiens 36-45 12963350-5 2003 The soluble recombinant fusion protein failed to undergo autocatalytic cleavage and activation; however, autocatalytic cleavage and activation of recombinant human enteropeptidase light chain (L-HEP) were achieved by solubilization and renaturation of the fusion protein from inclusion bodies and the active L-HEP was purified on agarose-linked soybean trypsin inhibitor. Sepharose 330-337 transmembrane serine protease 15 Homo sapiens 164-179 15969086-5 2003 The arresten cDNA from pGEM-T vector was recombined with vector pPIC9 as pPIC9-arresten, used to transform E. coli DH5alpha, and the inserted arresten cDNA confirmed by agarose electrophoresis and sequencing. Sepharose 169-176 collagen type IV alpha 1 chain Homo sapiens 4-12 12954221-4 2003 Transcription factors Sp1 and Sp3 were found to be KCS-binding proteins by electrophoretic mobility shift analyses (EMSA) and Sepharose bead-KCS oligonucleotide pull-down assays. Sepharose 126-135 Sp3 transcription factor Homo sapiens 30-33 12954221-4 2003 Transcription factors Sp1 and Sp3 were found to be KCS-binding proteins by electrophoretic mobility shift analyses (EMSA) and Sepharose bead-KCS oligonucleotide pull-down assays. Sepharose 126-135 tubulin folding cofactor E Homo sapiens 141-144 12799384-10 2003 Furthermore, we also found that the SHAP-HA complex tends to form aggregates, judging from the migration and elution profiles in agarose gel electrophoresis and gel filtration, respectively. Sepharose 129-136 inter-alpha-trypsin inhibitor heavy chain 1 Homo sapiens 36-40 12930694-6 2003 The relative density ratio of GATA-3 to GAPDH was 0.618 +/- 0.137 in nasal polyp and 0.21 +/- 0.11 in normal nasal mucosa (P < 0.05) as indicated by RT-PCR and agarose electrophoresis. Sepharose 163-170 GATA binding protein 3 Homo sapiens 30-36 12906790-10 2003 This activation is specifically blocked by a synthetic peptide corresponding to the Asn-Pro-X-X-Tyr motif found in rhodopsin, and Rac-1 coprecipitates with rhodopsin on Concanavalin A Sepharose. Sepharose 184-193 rhodopsin Homo sapiens 115-124 12906790-10 2003 This activation is specifically blocked by a synthetic peptide corresponding to the Asn-Pro-X-X-Tyr motif found in rhodopsin, and Rac-1 coprecipitates with rhodopsin on Concanavalin A Sepharose. Sepharose 184-193 Rac family small GTPase 1 Homo sapiens 130-135 12906790-10 2003 This activation is specifically blocked by a synthetic peptide corresponding to the Asn-Pro-X-X-Tyr motif found in rhodopsin, and Rac-1 coprecipitates with rhodopsin on Concanavalin A Sepharose. Sepharose 184-193 rhodopsin Homo sapiens 156-165 12887298-4 2003 The MBL process includes three chromatographic steps, where the first and key step is affinity chromatography on a cross-linked agarose matrix selecting for oligomeric, carbohydrate-binding MBL. Sepharose 128-135 mannose binding lectin 2 Homo sapiens 4-7 12593674-7 2003 TTR, which was eluted in 60 kDa fractions during urea/Sephadex G-100 chromatography, was further purified to homogeneity using a combination of two dye-affinity chromatographic steps on Reactive Yellow and Cibacron Blue coupled to agarose columns. Sepharose 231-238 transthyretin Homo sapiens 0-3 12850144-10 2003 Transcription factor NFATc1 isoforms in nuclear extract were co-precipitated with the biotinylated AP-3 site by immobilized agarose beads and the genomic DNA containing PSME was precipitated by antibodies reactive to NFATc1, demonstrating that NFATc1 isoforms bound to the AP-3 site in PSME in vivo. Sepharose 124-131 nuclear factor of activated T cells 1 Homo sapiens 21-27 14514373-3 2003 The length polymorphism on AR gene was demonstrated by denaturing polyacrylamide gel electrophoresis and silver staining, the PGK gene products were treated with Bst XI and resolved on agarose gels. Sepharose 185-192 androgen receptor Homo sapiens 27-29 12756246-4 2003 In vitro reactions between wild-type and truncated HIV-1 Gag and human LysRS were monitored using GST-tagged molecules and glutathione-agarose chromatography. Sepharose 135-142 Pr55(Gag) Human immunodeficiency virus 1 57-60 12730206-5 2003 Both native and recombinant decorin can bind to SP-D that is already bound to maltose-agarose matrix, and these SP-D-decorin complexes are dissociated at high salt (0.5-1.0 m NaCl) conditions, releasing the decorin. Sepharose 86-93 surfactant protein D Homo sapiens 48-52 12660150-4 2003 In the absence of microsomal vesicles, the percentage of binding to Cbl-Sepharose matrix by TC II expressed by constructs C3S, C3/147/S, C98/147/S, or C3/98/147/S was 100, 49, 52, and 35%, respectively. Sepharose 72-81 transcobalamin 2 Homo sapiens 92-97 12883632-8 2003 The purified fusion protein inhibited urokinase-type plasminogen activator as measured by milk-agarose plate assay, and bound to human lung cancer cells via uPA receptor (uPAR), which was confirmed by radio competition experiments. Sepharose 95-102 plasminogen activator, urokinase Homo sapiens 38-74 12829179-8 2003 Agarose gel electrophoresis demonstrated two different N2BA titin isoforms in all rat ventricles. Sepharose 0-7 titin Rattus norvegicus 60-65 12835755-2 2003 To test the latter idea, we developed an in vitro assay, using biotinylated telomeric DNA probes and streptavidin-agarose, to quantify the ability of TRF1 and TIN2 to stimulate interactions of telomeric DNA tracts with each other (probe clustering). Sepharose 114-121 telomeric repeat binding factor 1 Homo sapiens 150-154 12862420-8 2003 To preserve the collagen-binding function of FN, it was pegylated while bound to a gelatin agarose matrix. Sepharose 91-98 fibronectin 1 Homo sapiens 45-47 12684504-5 2003 Hybrid apolipoprotein and apoE3-N-terminal, but not apoLp-III, bound to heparin-Sepharose. Sepharose 80-89 apolipoprotein E Homo sapiens 7-21 12783853-4 2003 This study documents the detection of fork collision in an fob1 derivative with reduced rDNA copy number (approximately 20) using two-dimensional agarose gel electrophoresis. Sepharose 146-153 replication fork barrier binding protein FOB1 Saccharomyces cerevisiae S288C 59-63 12921558-6 2003 RESULTS: The fragment of 980 bp (4-1BBL) and 5.4 kb (PCI-neo) was shown after PCI-neo-4-1BBL had been digested by EcoR I and Not I and agarose gel electrophoresis. Sepharose 135-142 TNF superfamily member 9 Homo sapiens 33-39 12921558-6 2003 RESULTS: The fragment of 980 bp (4-1BBL) and 5.4 kb (PCI-neo) was shown after PCI-neo-4-1BBL had been digested by EcoR I and Not I and agarose gel electrophoresis. Sepharose 135-142 serpin family A member 5 Homo sapiens 53-56 12921558-6 2003 RESULTS: The fragment of 980 bp (4-1BBL) and 5.4 kb (PCI-neo) was shown after PCI-neo-4-1BBL had been digested by EcoR I and Not I and agarose gel electrophoresis. Sepharose 135-142 serpin family A member 5 Homo sapiens 78-81 12921558-6 2003 RESULTS: The fragment of 980 bp (4-1BBL) and 5.4 kb (PCI-neo) was shown after PCI-neo-4-1BBL had been digested by EcoR I and Not I and agarose gel electrophoresis. Sepharose 135-142 TNF superfamily member 9 Homo sapiens 86-92 12684504-5 2003 Hybrid apolipoprotein and apoE3-N-terminal, but not apoLp-III, bound to heparin-Sepharose. Sepharose 80-89 apolipoprotein E Homo sapiens 26-31 12697314-4 2003 This pentraxin-like protein was isolated from serum by calcium-dependent binding to agarose. Sepharose 84-91 pentraxin Oncorhynchus mykiss 5-14 12736188-5 2003 Although native SOD2 has no affinity for heparin, SOD2/3 binds to a heparin-agarose column. Sepharose 76-83 superoxide dismutase 2 Rattus norvegicus 50-56 12833389-8 2003 The interaction of leukemic T-cells with free SBA, as well as with SBA-conjugated Sepharose beads, was examined fluorimetrically and visualized by fluorescent microscopy, using FITC-SBA as a marker. Sepharose 82-91 lectin Glycine max 67-70 12833389-8 2003 The interaction of leukemic T-cells with free SBA, as well as with SBA-conjugated Sepharose beads, was examined fluorimetrically and visualized by fluorescent microscopy, using FITC-SBA as a marker. Sepharose 82-91 lectin Glycine max 67-70 12833389-10 2003 Both normal lymphocytes and leukemic T-cells were removed in a mixture from SBA-free Sepharose 6MB by PBS(-) and were not fractionated discretely. Sepharose 85-94 lectin Glycine max 76-79 12651853-6 2003 To evaluate this hypothesis, we utilized a "pull-down" assay in which we identified, by Western analysis, the proteins in a rat kidney medullary homogenate that complexed with glutathione S-transferase (GST) fusion syntaxin isoforms attached to Sepharose 4B-glutathione beads. Sepharose 245-257 hematopoietic prostaglandin D synthase Rattus norvegicus 176-201 12803616-4 2003 Calmodulin (CaM) antagonist W7-agarose and anti-CaM antibody depressed La3+-promoted pollen germination and tube growth in a dose-dependent manner. Sepharose 31-38 calmodulin 1 Homo sapiens 0-10 12803616-4 2003 Calmodulin (CaM) antagonist W7-agarose and anti-CaM antibody depressed La3+-promoted pollen germination and tube growth in a dose-dependent manner. Sepharose 31-38 calmodulin 1 Homo sapiens 12-15 12856608-3 2003 The generated recombinant TFPI (rTFPI) could be simply purified with glutathione-agarose affinity method and maintained its biological function in terms of inhibition of tissue factor and factor Xa. Sepharose 81-88 tissue factor pathway inhibitor Homo sapiens 26-30 12884737-6 2003 The extent of vWF degradation was assayed by electrophoresis in SDS-agarose gels and immunoblotting. Sepharose 68-75 von Willebrand factor Homo sapiens 14-17 12783444-2 2003 A 1% vertical sodium dodecyl sulfate (SDS)-agarose gel electrophoresis (VAGE) system has been developed that allows titin (a protein with the largest known SDS subunit size of 3000-4000 kDa) to migrate over 10 cm in a approximately 13 cm resolving gel. Sepharose 43-50 titin Oryctolagus cuniculus 116-121 12857385-3 2003 First, the 5 biotinylated CCL22 analogues synthesized were captured by agarose-immobilized streptavidin, indicating that the biotin molecules introduced in positions G1, K27, K49, K61, and K66 of CCL22 were accessible for binding. Sepharose 71-78 C-C motif chemokine ligand 22 Homo sapiens 26-31 12857385-3 2003 First, the 5 biotinylated CCL22 analogues synthesized were captured by agarose-immobilized streptavidin, indicating that the biotin molecules introduced in positions G1, K27, K49, K61, and K66 of CCL22 were accessible for binding. Sepharose 71-78 keratin 27 Homo sapiens 170-173 12651853-6 2003 To evaluate this hypothesis, we utilized a "pull-down" assay in which we identified, by Western analysis, the proteins in a rat kidney medullary homogenate that complexed with glutathione S-transferase (GST) fusion syntaxin isoforms attached to Sepharose 4B-glutathione beads. Sepharose 245-257 hematopoietic prostaglandin D synthase Rattus norvegicus 203-206 12894505-6 2003 MATERIALS AND METHODS: The interaction of Raf-1 with Ras was examined by investigating the association of cytosolic Raf-1, from ET-18-OCH3-treated and untreated cells with purified GST-Ras-GTP-gamma-S bound to agarose beads. Sepharose 210-217 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 42-47 12834286-0 2003 Purification, characterization, and sequence analysis of two alpha-amylase isoforms from azuki bean, Vigna angularis, showing different affinity towards beta-cyclodextrin sepharose. Sepharose 171-180 alpha-amylase Vigna angularis 61-74 15969009-3 2003 After induction with IPTG, the GST-Ecp fusion protein from the lysate was bound to glutathione-Sepharose 4B and digested with thrombin. Sepharose 95-104 ecp Drosophila melanogaster 35-38 12709070-3 2003 In the present work we have expressed the second Kunitz-type protease inhibitor domain of the human protein WFIKKN in Escherichia coli, purified it by affinity chromatography on trypsin-Sepharose and its structure was characterized by CD spectroscopy. Sepharose 186-195 WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 1 Homo sapiens 108-114 12879166-3 2003 (35)[S]-TC II complexed with cobalamin (Cbl; Vitamin B(12)) bound to Sepharose-megalin affinity matrix and the binding was enhanced 5-fold when TC II-R was prebound to megalin. Sepharose 69-78 transcobalamin 2 Homo sapiens 8-13 12879166-3 2003 (35)[S]-TC II complexed with cobalamin (Cbl; Vitamin B(12)) bound to Sepharose-megalin affinity matrix and the binding was enhanced 5-fold when TC II-R was prebound to megalin. Sepharose 69-78 LDL receptor related protein 2 Homo sapiens 79-86 12811499-2 2003 GST isoenzyme(s) were first separated on the basis of their affinity to glutathione sepharose 4B affinity column. Sepharose 84-96 glutathione S-transferase kappa 1 Homo sapiens 0-3 12650758-4 2003 Indeed, nPhl p 6 could be highly enriched in one step using nickel-chelating Sepharose. Sepharose 77-86 S100 calcium binding protein A12 Homo sapiens 13-16 18758708-4 2003 Protein-protein binding experiments using CaM-Sepharose affinity media revealed that G(o)alpha GDP bound GAP-43 directly to form intermolecular complex. Sepharose 46-55 growth associated protein 43 Bos taurus 105-111 12814271-1 2003 Agarose based immobilized metal affinity chromatography (IMAC) columns loaded with copper (II) were evaluated for the selection of histidine-containing peptides in comparative proteomics. Sepharose 0-7 C-C motif chemokine ligand 26 Homo sapiens 57-61 12699876-6 2003 Uterine and placental PAF receptor expression was analyzed by reverse transcription-polymerase chain reaction and agarose gel electrophoresis. Sepharose 114-121 PCNA clamp associated factor Rattus norvegicus 22-25 12700583-4 2003 RESULTS: Under resting conditions, Sepharose column-isolated platelets showed a negligible fraction of only 2.7 +/- 3.3% cells (mean +/- SEM) with P-selectin expression, and an appropriate response (i.e., a 33-fold increase) upon activation with thrombin receptor-activating peptide (TRAP). Sepharose 35-44 selectin P Rattus norvegicus 147-157 12899756-10 2003 GST-IFIT1 fusion protein was further purified using Glutathione Sepharose 4B column, and was treated as bait to capture prey from peripheral white blood cell lysate of SLE patients. Sepharose 64-73 interferon induced protein with tetratricopeptide repeats 1 Homo sapiens 0-9 12591924-3 2003 The abnormal antithrombin was separated from the normal inhibitor by complexing the latter with thrombin followed by heparin-agarose affinity chromatography. Sepharose 125-132 serpin family C member 1 Homo sapiens 13-25 12591924-3 2003 The abnormal antithrombin was separated from the normal inhibitor by complexing the latter with thrombin followed by heparin-agarose affinity chromatography. Sepharose 125-132 coagulation factor II, thrombin Homo sapiens 17-25 12694871-4 2003 Binding of active, latent, and in vitro-activated p53 protein to DNA fragments modified by antitumor cisplatin was studied using electrophoretic mobility shift assay in agarose gels and immunoblotting analysis. Sepharose 169-176 tumor protein p53 Homo sapiens 50-53 12670497-2 2003 This interaction between importin 9 and the A subunit was confirmed by in vitro pulldown, immunoprecipitation, and microcystin-Sepharose chromatography. Sepharose 127-136 importin 9 Homo sapiens 25-35 12676354-2 2003 Agarose gel electrophoresis of reverse transcription-polymerase chain reaction (RT-PCR)-amplified products resulted in a single band of the expected size for each product with nucleotide sequences corresponding to AC1 to AC9. Sepharose 0-7 adenylate cyclase 1 Rattus norvegicus 214-217 12817620-1 2003 We previously reported that proper dilution of sera that contain IgG monoclonal proteins (M-proteins) is necessary for accurate quantitation of serum albumin and M-protein concentrations separated by serum protein electrophoresis (SPE) using the Beckman PARAGON agarose electrophoresis system. Sepharose 262-269 myomesin 2 Homo sapiens 90-99 12910306-2 2003 Single or 2-fold incubation with tumor necrosis factor-a activated and preactivated human blood macrophages and promoted oxidative modification of low-density lipoproteins (increased their mobility in agarose gel). Sepharose 201-208 tumor necrosis factor Rattus norvegicus 33-56 12747597-4 2003 Screening for the I/D ACE and A1166C AT1R genotypes was performed by polymerase chain reaction of genomic DNA, followed by restriction enzyme digestion and agarose gel electrophoresis. Sepharose 156-163 angiotensin II receptor type 1 Homo sapiens 37-41 22900303-1 2003 Intrinsic factor (IF) from human gastric juice was purified and complexed with vitamin B12 (IF-B12 complex) on Sepharose-vitamin B12 affinity matrix. Sepharose 111-120 cobalamin binding intrinsic factor Homo sapiens 0-16 22900303-1 2003 Intrinsic factor (IF) from human gastric juice was purified and complexed with vitamin B12 (IF-B12 complex) on Sepharose-vitamin B12 affinity matrix. Sepharose 111-120 cobalamin binding intrinsic factor Homo sapiens 18-20 12651006-7 2003 Previously described purifications include ATP-agarose chromatography leading to Ssa1p partially complexed with ATP. Sepharose 47-54 Hsp70 family ATPase SSA1 Saccharomyces cerevisiae S288C 81-86 12901825-2 2003 METHODS: ACE I/D polymorphism was performed on DNA samples from patients using the polymerase chain reaction (PCR) followed by agarose gel electrophoresis. Sepharose 127-134 angiotensin I converting enzyme Homo sapiens 9-12 12615392-6 2003 Results with this strain show that sufficient levels of nuclease activity are produced to completely auto-hydrolyse the host"s chromosomal DNA to a size non-visible on 1% agarose gel, generating a markedly lower lysate viscosity. Sepharose 171-178 nuclease Escherichia coli 56-64 12623069-1 2003 Purified human placental transcobalamin II receptor (TC II-R) dimer of molecular mass 124 kDa bound to Sepharose-linked bacterial immunoglobulin (IgG) binding proteins protein A, protein G, and protein A/G. Sepharose 103-112 transcobalamin 2 Homo sapiens 53-58 12493752-3 2003 Using a prokaryotic expression system, full-length rat BSP was expressed and tested for HA nucleating activity in a steady-state agarose gel system. Sepharose 129-136 integrin-binding sialoprotein Rattus norvegicus 55-58 12444018-4 2003 The intact and modified COOH termini of NBC3 and the 56-kDa subunit of the proton pump were synthesized, coupled to Sepharose beads, and used to pull down kidney membrane proteins. Sepharose 116-125 solute carrier family 4 member 7 Rattus norvegicus 40-44 12715898-3 2003 In vitro pull-down experiments with calmodulin-Sepharose demonstrated a Ca2+-dependent interaction with cellularly expressed ERRgamma. Sepharose 47-56 calmodulin 1 Homo sapiens 36-46 12612978-4 2003 METHODS: Using lectin-binding properties, sialylation and galactosylation of serum IgA1, isolated on jacalin-conjugated agarose, were investigated in male and female patients with IgA nephropathy with T-GBM (n = 22) and N-GBM (n = 22) compared with matched (age and sex) healthy controls (n = 22). Sepharose 120-127 immunoglobulin heavy constant alpha 1 Homo sapiens 83-87 12715898-3 2003 In vitro pull-down experiments with calmodulin-Sepharose demonstrated a Ca2+-dependent interaction with cellularly expressed ERRgamma. Sepharose 47-56 estrogen related receptor gamma Homo sapiens 125-133 12733967-3 2003 Newly revealed cytosolic tankyrase in its poly(ADP-ribosyl)ated form was passed through a Sepharose 2B column and eluted as an apparently monomeric protein. Sepharose 90-99 tankyrase Homo sapiens 25-34 15151759-6 2003 LAIR1-Ig fusion protein was purified through affinity column anti-Fc mAb cross-linked to Sepharose 4B. Sepharose 89-98 leukocyte-associated Ig-like receptor 1 Mus musculus 0-5 12605705-6 2003 Human anti-SSB/La autoantibodies purified from active SLE sera passing through the recombinant SSB/La conjugated Sepharose 4B affinity column could bind and penetrate into normal human PMN. Sepharose 113-122 small RNA binding exonuclease protection factor La Homo sapiens 11-14 12605705-6 2003 Human anti-SSB/La autoantibodies purified from active SLE sera passing through the recombinant SSB/La conjugated Sepharose 4B affinity column could bind and penetrate into normal human PMN. Sepharose 113-122 small RNA binding exonuclease protection factor La Homo sapiens 95-98 15151743-3 2003 The extracellular region gene of LAIR1 and LAIR-2 were inserted into vector pGEX-4T-3 expressing GST fusion protein, expressed on IPTG induction and purified through glutathione-sepharose 4B column. Sepharose 178-187 leukocyte associated immunoglobulin like receptor 1 Homo sapiens 33-38 15151743-3 2003 The extracellular region gene of LAIR1 and LAIR-2 were inserted into vector pGEX-4T-3 expressing GST fusion protein, expressed on IPTG induction and purified through glutathione-sepharose 4B column. Sepharose 178-187 leukocyte associated immunoglobulin like receptor 2 Homo sapiens 43-49 12573445-4 2003 In the present study, the PA-PLA(1) was solubilized in Triton X-100 from membranes pre-treated with 1 M NaCl, and purified 280-fold from platelet homogenates by sequential chromatography on blue-Toyopearl, red-Toyopearl, DEAE-Toyopearl, green-agarose, brown-agarose, polylysine-agarose, palmitoyl-CoA-agarose and blue-5PW columns. Sepharose 243-250 DDHD domain containing 1 Homo sapiens 26-35 12573445-4 2003 In the present study, the PA-PLA(1) was solubilized in Triton X-100 from membranes pre-treated with 1 M NaCl, and purified 280-fold from platelet homogenates by sequential chromatography on blue-Toyopearl, red-Toyopearl, DEAE-Toyopearl, green-agarose, brown-agarose, polylysine-agarose, palmitoyl-CoA-agarose and blue-5PW columns. Sepharose 258-265 DDHD domain containing 1 Homo sapiens 26-35 12443858-2 2003 After adsorption by the solid phase, Q Sepharose Fast Flow media, bovine serum albumin (BSA) molecules were allowed to react with glutaraldehyde. Sepharose 39-48 albumin Bos taurus 88-91 12601725-1 2003 Rapid and highly reproducible nonreducing agarose gel electrophoresis (NRAGE) of von Willebrand Factor (vWF) multimers was performed using a thermostated minigel apparatus that monitors and precisely controls internal gel temperature. Sepharose 42-49 von Willebrand factor Homo sapiens 81-102 12601725-1 2003 Rapid and highly reproducible nonreducing agarose gel electrophoresis (NRAGE) of von Willebrand Factor (vWF) multimers was performed using a thermostated minigel apparatus that monitors and precisely controls internal gel temperature. Sepharose 42-49 von Willebrand factor Homo sapiens 104-107 12592549-0 2003 Induction of matrix metalloproteinase-2 and -3 activity in ovine nucleus pulposus cells grown in three-dimensional agarose gel culture by interleukin-1beta: a potential pathway of disc degeneration. Sepharose 115-122 matrix metallopeptidase 2 Homo sapiens 13-46 12592549-0 2003 Induction of matrix metalloproteinase-2 and -3 activity in ovine nucleus pulposus cells grown in three-dimensional agarose gel culture by interleukin-1beta: a potential pathway of disc degeneration. Sepharose 115-122 interleukin 1 beta Homo sapiens 138-155 12630697-3 2003 The ribonuclease was adsorbed on CM-Sepharose and Mono S. Sepharose 36-45 ribonuclease Saccharomyces cerevisiae S288C 4-16 12525607-5 2003 In in vitro cell culture assays, RRV ORF74 possesses transforming potential, as NIH 3T3 clones stably expressing the receptor demonstrate an increased ability to grow in soft agarose and to induce tumor formation in nude mice. Sepharose 175-182 G protein coupled receptor Macacine gammaherpesvirus 5 37-42 12591496-0 2003 Protection of NOD islet isograft from autoimmune destruction by agarose microencapsulation. Sepharose 64-71 atrophin 1 Homo sapiens 14-17 12573445-4 2003 In the present study, the PA-PLA(1) was solubilized in Triton X-100 from membranes pre-treated with 1 M NaCl, and purified 280-fold from platelet homogenates by sequential chromatography on blue-Toyopearl, red-Toyopearl, DEAE-Toyopearl, green-agarose, brown-agarose, polylysine-agarose, palmitoyl-CoA-agarose and blue-5PW columns. Sepharose 258-265 DDHD domain containing 1 Homo sapiens 26-35 15151759-10 2003 LAIR1-Ig fusion protein was effectively purified through FMUFc5-Sepharose affinity chromatography column. Sepharose 64-73 leukocyte-associated Ig-like receptor 1 Mus musculus 0-5 12450545-0 2003 Purification of angiotensin I converting enzyme from pig lung using concanavalin-A sepharose chromatography. Sepharose 83-92 angiotensin-converting enzyme Sus scrofa 16-47 12559961-7 2003 The recombinant CSP oligomers as well as the CSP monomers directly associate with Ni(2+)-NTA agarose. Sepharose 93-100 DnaJ heat shock protein family (Hsp40) member C5 Homo sapiens 16-19 12559961-7 2003 The recombinant CSP oligomers as well as the CSP monomers directly associate with Ni(2+)-NTA agarose. Sepharose 93-100 DnaJ heat shock protein family (Hsp40) member C5 Homo sapiens 45-48 12493574-3 2003 The highest percentage of apoptotic cells accumulated to 27.85 +/- 9.2% following pretreatment with Cort 10 microM for 5 d. In agarose gel electrophoresis of DNA, the sample obtained from PC12 cells pretreated with Cort 10 microM for 5 d showed a typical ladder pattern suggesting that Cort increased the DNA fragmentation significantly. Sepharose 127-134 cortistatin Rattus norvegicus 100-104 12403778-6 2003 Removal of LPS from rhHsp70-2 by polymyxin B-agarose column or direct addition of polymyxin B to the incubation medium essentially eliminated the TNFalpha-inducing activity of rhHsp70-2. Sepharose 45-52 toll-like receptor 4 Mus musculus 11-14 12450545-2 2003 In this report we describe a two-step affinity chromatography method for preparative purification of ACE from pig lung using Concanavalin-A Sepharose 4B and affinity chromatography on Lisinopril Sepharose 6B. Sepharose 140-149 angiotensin-converting enzyme Sus scrofa 101-104 12561428-9 2003 After treatment with ASODN/Lip for 36 hours, most cells stained with Hoechst 33258/Pl exhibited apoptotic cell morphology such as cell shrinkage, nuclear condensation, and nuclear fragmentation under fluorescence microscope; a apoptotic peak appeared on flow cytometry; a ladder-like pattern of DNA fragmentation appeared on agarose gel electrophoresis. Sepharose 325-332 SMG1 nonsense mediated mRNA decay associated PI3K related kinase Homo sapiens 27-30 12952170-5 2003 cDNA was amplified by PCR and OPN mRNA was revealed on agarose gels. Sepharose 55-62 secreted phosphoprotein 1 Mus musculus 30-33 12519402-7 2003 Affinity chromatography on rCR1-sepharose depleted the plasma of antiCR1, and the acid-eluted fractions contained the antiCR1 Ab. Sepharose 32-41 complement C3b/C4b receptor 1 like Rattus norvegicus 27-31 14577450-7 2003 The numerical results show that gellified lysozyme (crystals "locked"on the matrix of agarose gel) precipitates to produce "spaced deposits". Sepharose 86-93 lysozyme Homo sapiens 42-50 14617825-4 2003 One to three simultaneously amplified DNA fragments of HMW glutenin Glu-1 genes were separated by agarose slab-gel electrophoresis and differences between Ax1, Ax2* and Axnull genes of Glu-A1 loci, Bx6, Bx7 and Bx17 of Glu-B1, and Dx2, Dx5 and Dy10 genes of Glu-D1 loci were revealed. Sepharose 98-105 HMW glutenin subunit Triticum aestivum 55-67 12566663-7 2003 RESULTS: Anti-gp120 agarose removed approximately 90% of HIV-1 gp120 from HL2/3 cultures in 30-60 min. Sepharose 20-27 Envelope surface glycoprotein gp160, precursor Human immunodeficiency virus 1 14-19 12566663-7 2003 RESULTS: Anti-gp120 agarose removed approximately 90% of HIV-1 gp120 from HL2/3 cultures in 30-60 min. Sepharose 20-27 Envelope surface glycoprotein gp160, precursor Human immunodeficiency virus 1 63-68 12566663-7 2003 RESULTS: Anti-gp120 agarose removed approximately 90% of HIV-1 gp120 from HL2/3 cultures in 30-60 min. Sepharose 20-27 dynein cytoplasmic 1 heavy chain 1 Homo sapiens 74-79 14674248-9 2003 Moreover, covalent coupling of affinity-purified sheep antibodies to agarose provided a means for the immuno-isolation of PP1 beta and PP1 gamma 1 from the PPC preparation. Sepharose 69-76 protein phosphatase 1 catalytic subunit beta Homo sapiens 122-146 12566913-5 2003 The VDR gene polymorphisms were detected by the presence or absence of the particular restriction site using agarose gel electrophoresis. Sepharose 109-116 vitamin D receptor Homo sapiens 4-7 12488049-1 2002 A soluble form of the HIV-1 envelope glycoprotein gp160 devoid of the transmembrane anchor domain was found to bind to cholesteryl-hemisuccinate agarose. Sepharose 145-152 Envelope surface glycoprotein gp160, precursor Human immunodeficiency virus 1 50-55 12756893-5 2003 Recently, marked progress in biochemistry, molecular biology and genetics provided researchers of apoptosis various tools for apoptosis detection, such as the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling (TUNEL) method, agarose gel electrophoresis using extracted DNA, staining methods using fluorescence dyes, and flow cytometry. Sepharose 258-265 DNA nucleotidylexotransferase Homo sapiens 198-201 12509979-2 2003 The p68 subunit is expressed, in the absence of induction, and use of a heparin-Sepharose column produces substantially pure protein. Sepharose 80-89 GATA zinc finger domain containing 2B Homo sapiens 4-7 15639817-4 2003 The recombinant immunotoxin BDI(scFv)-PE38 was expressed mainly in soluble form and can be purified by Ni2+-NTA agarose. Sepharose 112-119 immunglobulin heavy chain variable region Homo sapiens 32-36 16212561-2 2002 We have purified cystatin C from urine of patients with chronic renal failure by procedure using affinity chromatography on CM-papain Sepharose, gel filtration on Sephacryl S-200, and ion exchange chromatography on CM-cellulose. Sepharose 134-143 cystatin C Homo sapiens 17-27 12465987-3 2002 To study this interaction, agarose beads carrying gelatin (a proline-rich protein) were placed in the IR flow cell in such a way that the beads were probed by the IR beam. Sepharose 27-34 complement component 4 binding protein alpha Homo sapiens 61-81 12472579-3 2002 We used immunoadsorption on protein A Sepharose (IA-PA), which has been shown to be efficient in decreasing the titre of antibodies in several immune diseases, in three patients with Glanzmann"s thrombasthenia and anti-GPIIb-IIIa isoantibodies on five different occasions. Sepharose 38-47 integrin subunit alpha 2b Homo sapiens 219-224 12477167-2 2002 A new method, which can detect basepair mismatches with RNase cleavage on agarose gel electrophoresis, coupled with DNA sequencing, identified 8 novel sequence variations in the NF-L gene. Sepharose 74-81 neurofilament light chain Homo sapiens 178-182 12445176-3 2002 The presence of macro-AST in serum was confirmed utilizing size-exclusion, high performance liquid chromatography (HPLC) and Protein G-agarose beads to precipitate immune complexes of AST and immunoglobulin G followed by centrifugation and AST activity measurements in the supernatant. Sepharose 135-142 solute carrier family 17 member 5 Homo sapiens 22-25 12239216-2 2002 We have searched the genome of the yeast Saccharomyces cerevisiae for open reading frames that encode proteins with putative PIP-boxes and initiated testing of 135 novel candidates for their ability to interact with PCNA-conjugated agarose beads. Sepharose 232-239 proliferating cell nuclear antigen Saccharomyces cerevisiae S288C 216-220 12513717-3 2002 After confirming by DNA sequencing analysis, the recombinant expression plasmid pQE31-vWF/A1 was constructed and introduced into E. coli M15 strain, then induced by IPTG; the expressed protein was purified with Ni-NTA agarose, identified by Western blotting. Sepharose 218-225 von Willebrand factor Homo sapiens 86-89 12445219-6 2002 SNPs in the IL-1alpha (+ 4845) and IL-1beta (- 511, + 3954) genes were analyzed by amplifying the polymorphic region using polymerase chain reaction (PCR), followed by restriction-enzyme digestion and agarose gel electrophoresis. Sepharose 201-208 interleukin 1 alpha Homo sapiens 12-21 12423363-9 2002 The protein was able to bind to ATP-Sepharose like the other molecular chaperone HSP70. Sepharose 36-45 heat shock protein family A (Hsp70) member 1B Rattus norvegicus 81-86 12194978-5 2002 In the current study, we employed alpha(2)-macroglobulin (alpha(2)M)-agarose column chromatography to purify cell surface membrane binding proteins from 1-LN human prostate cancer cells and murine macrophages. Sepharose 69-76 alpha-2-macroglobulin Homo sapiens 34-56 12194978-5 2002 In the current study, we employed alpha(2)-macroglobulin (alpha(2)M)-agarose column chromatography to purify cell surface membrane binding proteins from 1-LN human prostate cancer cells and murine macrophages. Sepharose 69-76 alpha-2-macroglobulin Homo sapiens 58-67 12417021-5 2002 The C4bp mutant with deletion of SCR2 lost the C4b-binding ability, as judged on C3b/C4b-Sepharose binding assaying and ELISA. Sepharose 89-98 complement component 4 binding protein alpha Homo sapiens 4-8 12417021-5 2002 The C4bp mutant with deletion of SCR2 lost the C4b-binding ability, as judged on C3b/C4b-Sepharose binding assaying and ELISA. Sepharose 89-98 RNA binding motif single stranded interacting protein 1 Homo sapiens 33-37 12417021-5 2002 The C4bp mutant with deletion of SCR2 lost the C4b-binding ability, as judged on C3b/C4b-Sepharose binding assaying and ELISA. Sepharose 89-98 complement C4B (Chido blood group) Homo sapiens 4-7 12417021-5 2002 The C4bp mutant with deletion of SCR2 lost the C4b-binding ability, as judged on C3b/C4b-Sepharose binding assaying and ELISA. Sepharose 89-98 complement C4B (Chido blood group) Homo sapiens 47-50 12429847-3 2002 Consistently, we have recently demonstrated, using fluorescence microscopy, that Myo5p is able to induce cytosol-dependent actin polymerization on the surface of Sepharose beads. Sepharose 162-171 myosin 5 Saccharomyces cerevisiae S288C 81-86 12458888-7 2002 Immunoabsorption and agarose-gel electrophoresis indicated that the dominant apoJ-containing lipoprotein partides did not contain apoE. Sepharose 21-28 clusterin Homo sapiens 77-81 12450113-6 2002 Sepharose-immobilized dCyt c had activity similar strength to that in solution. Sepharose 0-9 Cytochrome c distal Drosophila melanogaster 22-28 12523650-7 2002 A fusion protein of glutathione-S-transferase and human progastrin(1-80) was expressed in Escherichia coli, collected on glutathione-agarose beads, and cleaved with enterokinase. Sepharose 133-140 glutathione S-transferase kappa 1 Homo sapiens 20-45 12177048-7 2002 The observation that His-dMTase increases the demethylase activity in the cells was also confirmed using an in vitro CpG demethylase assay with a mC32pG oligonucleotide substrate and purified Q-Sepharose fractions from HEK293 cells transfected with His-dMTase or empty pcDNA3.1His vector. Sepharose 194-203 methyl-CpG binding domain protein 2 Homo sapiens 46-57 12213490-5 2002 In the adipose tissue of the untreated group, 72% of the LPL was the inactive-monomeric form, which was eluted with 0.4-0.75 M NaCl from the heparin-Sepharose column, and 28% was the active-dimeric form, which was eluted with 0.8-1.2 M NaCl. Sepharose 149-158 lipoprotein lipase Homo sapiens 57-60 12232052-1 2002 The ventral nervous system defective (vnd)/NK-2 homeodomain and some flanking amino acid residues were expressed in Escherichia coli, purified to homogeneity, and the protein was covalently coupled to Sepharose. Sepharose 201-210 ventral nervous system defective Drosophila melanogaster 43-47 12198170-3 2002 Some isoforms (e.g. IFE-3) bind to m(7)GTP-Sepharose exclusively, whereas others (e.g. IFE-5) bind to both m(7)GTP- and m(3)(2,2,7)GTP-Sepharose. Sepharose 43-52 Eukaryotic translation initiation factor 4E-3 Caenorhabditis elegans 20-25 12296378-1 2002 p28, a 28kD protein from toad (Bufo bufo gargarizans) oocytes, was identified by using p13(suc1)-agarose affinity chromatography. Sepharose 97-104 golgi SNAP receptor complex member 1 Homo sapiens 0-3 12230948-6 2002 Apoptotic DNA breaks were further confirmed by a typical "DNA ladder" on agarose gel electrophoresis after exposure to Des 40 micromol/L for 24 h. Meanwhile, expression of caspase 3 gene was observed following Des 20 micromol/L treatment. Sepharose 73-80 caspase 3 Rattus norvegicus 172-181 12009311-2 2002 Antithrombin III (ATIII) was purified from ostrich plasma by heparin-Sepharose and Super Q-650S chromatography. Sepharose 69-78 serpin family C member 1 Homo sapiens 18-23 12358748-6 2002 By employing liposome and calmodulin in a state of being either soluble or immobilized on agarose, actual competition of alpha-synuclein between membranes and calmodulin was demonstrated with the observation that alpha-synuclein previously bound to the liposome was released upon specific interaction with the calmodulins. Sepharose 90-97 synuclein alpha Homo sapiens 121-136 12052833-11 2002 Lutein-binding capacity of the recombinant CBP (rCBP) determined by incubating rCBP with lutein followed by immunoprecipitation using anti-CBP IgG conjugated to protein A-Sepharose, demonstrated the formation of a lutein-rCBP complex. Sepharose 171-180 carotenoid-binding protein Bombyx mori 43-46 12442545-4 2002 Genotypes of ACE gene were determined by polymeraze chain reaction, followed with electrophoresis in agarose gel. Sepharose 101-108 angiotensin I converting enzyme Homo sapiens 13-16 12665935-7 2002 Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. Sepharose 95-107 interferon alpha 1 Homo sapiens 77-86 12111188-5 2002 The presence of anti-prolactin auto-antibodies was suspected because of low recovery of PRL after precipitation with polyethylene glycol and confirmed by immunoprecipitation with anti-human IgG-agarose. Sepharose 194-201 prolactin Homo sapiens 21-30 12127330-0 2002 Quantitative analysis of IgA1 binding protein prepared from human serum by hypoglycosylated IgA1/Sepharose affinity chromatography. Sepharose 97-106 immunoglobulin heavy constant alpha 1 Homo sapiens 25-29 12127330-1 2002 The binding protein to a hypoglycosylated IgA1/Sepharose (IgA1-BP) could be prepared from human sera. Sepharose 47-56 immunoglobulin heavy constant alpha 1 Homo sapiens 42-46 12127330-1 2002 The binding protein to a hypoglycosylated IgA1/Sepharose (IgA1-BP) could be prepared from human sera. Sepharose 47-56 immunoglobulin heavy constant alpha 1 Homo sapiens 58-62 12034717-8 2002 Anti-FLAG-agarose specifically precipitated GRIF-1(FLAG) and GABA(A) receptor beta 2 subunits from human embryonic kidney 293 cells co-transfected with GRIF-1a(FLAG) and beta 2 subunit clones. Sepharose 10-17 trafficking kinesin protein 2 Homo sapiens 44-50 12112671-7 2002 These experiments included the purification of the Z(SPA-1) affibody from a total Escherichia coli cell lysate using protein A-Sepharose, suggesting that this protein A/antiprotein A affinity pair could provide a basis for novel affinity gene fusion systems. Sepharose 127-136 signal-induced proliferation-associated 1 Homo sapiens 53-58 12113980-6 2002 Especially in case of the Sepharose Q FF, Mono Q 5/5 HR and Hitrap Q XL columns the vanadium-transferrin binding was degraded during elution on the column. Sepharose 26-35 transferrin Homo sapiens 93-104 12180976-4 2002 Subcellular fractionation and precipitation by GTP-agarose demonstrated that S64/SBP2 is a membrane-associated protein that exhibits GTP binding activity. Sepharose 51-58 sucrose-binding protein 2 Glycine max 81-85 12186740-7 2002 Restriction enzyme digestion of 926 bp genomic fragments of all the isolates including Cux-1 isolate with HindIII exhibited a similar pattern of bands in 2% agarose gel. Sepharose 157-164 cut like homeobox 1 Homo sapiens 87-92 12230885-4 2002 The caspase-3 enzyme was cast into a thin agarose gel, which was placed on a sheet containing test compounds. Sepharose 42-49 caspase 3 Homo sapiens 4-13 12225811-5 2002 This interaction is further demonstrated in vivo by colocalization of both COMP and fibronectin in the chondrocyte pericellular matrix by laser confocal microscopy of chondrocytes grown in agarose culture, and by appositional and colocalization of these proteins in the growth plate of primates by immunohistochemistry. Sepharose 189-196 cartilage oligomeric matrix protein Homo sapiens 75-79 12182818-5 2002 Decorin was isolated by a second Q-Sepharose chromatography with affinity chromatographies on heparin-Sepharose and concanavalin A-Sepharose. Sepharose 35-44 decorin Bos taurus 0-7 12182818-5 2002 Decorin was isolated by a second Q-Sepharose chromatography with affinity chromatographies on heparin-Sepharose and concanavalin A-Sepharose. Sepharose 102-111 decorin Bos taurus 0-7 12182818-5 2002 Decorin was isolated by a second Q-Sepharose chromatography with affinity chromatographies on heparin-Sepharose and concanavalin A-Sepharose. Sepharose 102-111 decorin Bos taurus 0-7 12467533-3 2002 The rat hepatocytic insulin receptor was partially purified by wheat-germ agglutinin (WGA)-sepharose 4B affinity chromatography. Sepharose 91-103 insulin receptor Rattus norvegicus 20-36 12100037-7 2002 In normal IgG this autoreactivity could be adsorbed almost completely on F(ab")2 fragments from the same IgG preparation, coupled to Sepharose and could be inhibited by the effluent obtained after subjecting normal IgG to HSP90 affinity column. Sepharose 133-142 heat shock protein 90 alpha family class A member 1 Homo sapiens 222-227 12270764-10 2002 Affinity chromatography with heparin Sepharose demonstrates that cTnT and cTnI interact differentially with the negatively charged ligand. Sepharose 37-46 troponin T2, cardiac type Homo sapiens 65-69 12270764-10 2002 Affinity chromatography with heparin Sepharose demonstrates that cTnT and cTnI interact differentially with the negatively charged ligand. Sepharose 37-46 troponin I3, cardiac type Homo sapiens 74-78 12354544-4 2002 Using agarose and polyacrylamide gel electrophoresis of canine serum or plasma, we detected a cathodal, slow form of A2M. Sepharose 6-13 alpha-2-macroglobulin-P Canis lupus familiaris 117-120 12193285-3 2002 MAb was purified by affinity chromatography on protein A-Sepharose, and MAb had a high affinity for trypsin-1 with the affinity constant equal 1.79 x 10(9) L/mol. Sepharose 57-66 serine protease 1 Homo sapiens 100-109 12417054-7 2002 The M235T angiotensinogen gene mutation was analysed by polymerase chain reaction followed by enzymatic digestion with Tth 111I restriction endonuclease enzyme and agarose gel electrophoresis of the products. Sepharose 164-171 angiotensinogen Homo sapiens 10-25 12354544-5 2002 Upon activation with elastase, slow A2M resembled normal A2M in agarose gel electrophoresis, showing decreased negative charge at semi-saturation but not at full saturation with enzyme. Sepharose 64-71 alpha-2-macroglobulin-P Canis lupus familiaris 36-39 12354544-8 2002 Incubation of slow A2M with ammonium sulphate, a known activator of AMs, resulted in decreased negative charge in agarose gel electrophoresis and no reaction or partial reaction in PAGE. Sepharose 114-121 alpha-2-macroglobulin-P Canis lupus familiaris 19-22 12127815-7 2002 HGF was purified from the liver and plasma on a SP-Sepharose column and was analyzed by Western blotting. Sepharose 51-60 hepatocyte growth factor Rattus norvegicus 0-3 12098759-6 2002 Primary studies showed that the fusion antigen could be specifically bind to and elute from anti-preS1 antibody coupled Sepharose resin, suggesting that large-scale preparation of the fusion antigen is feasible with an immunoaffinity resin. Sepharose 120-129 large envelope protein;middle envelope protein;small envelope protein Hepatitis B virus 97-102 12127836-11 2002 In agarose diffusion assays IGFBP-3 interacted with fibronectin and heparan sulfate proteoglycan but not with type VI collagen or tenascin-C. Sepharose 3-10 insulin like growth factor binding protein 3 Homo sapiens 28-35 12119116-10 2002 This residue is conserved in LPL from all mammals and has been shown to be critical for enzyme stability at 37 degrees C. On chromatography on heparin-Sepharose trout and chicken LPL eluted at higher salt concentration than bovine (or other mammalian) LPL. Sepharose 151-160 lipoprotein lipase Homo sapiens 29-32 12385237-7 2002 BL21 (DE3) respectively, we got 3 kinds of engineered E. coli which express TPO + Fc chimeric proteins(28 kD TPO + Fc1, 12 kD TPO + Fc2 and 12 kD TPO + Fc3) at high level respectively, the expressed proteins were purified with DEAE-Sepharose FF and S-Sepharose FF column. Sepharose 232-241 thrombopoietin Mus musculus 76-79 12051739-6 2002 Depletion of these cytosol extracts by microcystin-Sepharose maintained Bcl-2 phosphorylated forms, indicating that this cytosol possessed phosphatase activity. Sepharose 51-60 BCL2 apoptosis regulator Homo sapiens 72-77 12033828-2 2002 We show the commercial enzyme corresponds to the major peak 2 PME previously separated by heparin-Sepharose chromatography (Cameron et al., J. Sepharose 98-107 pectinesterase 2 Citrus sinensis 62-65 12244755-3 2002 RESULTS: DNA agarose gel electrophoresis results showed the characteristic "ladder" pattern of apoptosis in HPBLs treated with ST, DON and AFG1. Sepharose 13-20 AFG1 like ATPase Homo sapiens 139-143 12516782-2 2002 Making use of the surface biotinylation method, followed by the isolation of the GRP78 using ATP-agarose affinity chromatography, it was found that a fraction of the thapsigargin-induced GRP78 is expressed on the cell surface. Sepharose 97-104 heat shock protein family A (Hsp70) member 5 Homo sapiens 81-86 12516782-2 2002 Making use of the surface biotinylation method, followed by the isolation of the GRP78 using ATP-agarose affinity chromatography, it was found that a fraction of the thapsigargin-induced GRP78 is expressed on the cell surface. Sepharose 97-104 heat shock protein family A (Hsp70) member 5 Homo sapiens 187-192 12139427-5 2002 Extracts were tested with antisera for specific CPS types Ia, Ib, and II - VIII by double immunodiffusion (DD) in agarose. Sepharose 114-121 cytochrome c oxidase subunit 8A Homo sapiens 75-79 12475443-9 2002 Both anti-M-CSF-R monoclonal antibody and recombinant human M-CSF soluble receptor could cause the growth arrest of HL-60 cell in G(0)/G(1) phase, and could inhibit the formation of colony of HL-60 cell in soft agarose. Sepharose 211-218 colony stimulating factor 1 receptor Homo sapiens 10-17 12005511-0 2002 Immobilization of adenosine deaminase onto agarose and casein. Sepharose 43-50 adenosine deaminase Homo sapiens 18-37 12005511-1 2002 In the present study adenosine deaminase (ADA) was immobilized onto two different polymeric materials, agarose and casein. Sepharose 103-110 adenosine deaminase Homo sapiens 21-40 12005511-1 2002 In the present study adenosine deaminase (ADA) was immobilized onto two different polymeric materials, agarose and casein. Sepharose 103-110 adenosine deaminase Homo sapiens 42-45 12084065-7 2002 Following incubation of a cytosolic extract from nonactivated bovine neutrophil with protein A-Sepharose bound to anti-p67phox antibodies, the recovered immunoprecipitate contained the S100 protein, p47phox and p67phox. Sepharose 95-104 neutrophil cytosolic factor 2 Bos taurus 119-126 11973346-6 2002 Heparin-agarose-purified p33/30 was identified as histone H1. Sepharose 8-15 inhibitor of growth family member 1 Homo sapiens 25-28 11973346-6 2002 Heparin-agarose-purified p33/30 was identified as histone H1. Sepharose 8-15 H1.0 linker histone Homo sapiens 50-60 11884402-9 2002 Preferential association with the urea-denatured Tg-Sepharose was indicative of either direct or circuitous ERp29/Tg interactions in a chaperone-like manner. Sepharose 52-61 thyroglobulin Rattus norvegicus 49-51 11884402-9 2002 Preferential association with the urea-denatured Tg-Sepharose was indicative of either direct or circuitous ERp29/Tg interactions in a chaperone-like manner. Sepharose 52-61 endoplasmic reticulum protein 29 Rattus norvegicus 108-113 11884402-9 2002 Preferential association with the urea-denatured Tg-Sepharose was indicative of either direct or circuitous ERp29/Tg interactions in a chaperone-like manner. Sepharose 52-61 thyroglobulin Rattus norvegicus 114-116 11854286-8 2002 Both LA- and HA-PLTP bind to heparin-Sepharose and can be separated by elution with 0-0.5 m NaCl gradient, with HA-PLTP displaying higher affinity for the matrix. Sepharose 37-46 phospholipid transfer protein Homo sapiens 16-20 11854286-10 2002 HA-PLTP was subjected to a second heparin-Sepharose step and hydroxylapatite chromatography. Sepharose 42-51 phospholipid transfer protein Homo sapiens 3-7 12054550-3 2002 The ribonuclease was adsorbed on Affi-gel blue gel, CM-Sepharose, and Mono S. Sepharose 55-64 ribonuclease Saccharomyces cerevisiae S288C 4-16 11978773-10 2002 Recombinant m-Langerin could form multimers and bind to mannan-agarose. Sepharose 63-70 CD207 antigen Mus musculus 14-22 12038632-1 2002 Transforming growth factor-beta1 (TGF-beta1) has been shown to stimulate chondrogenesis in periosteal explants cultured in agarose suspension. Sepharose 123-130 transforming growth factor beta 1 Homo sapiens 0-32 12038632-1 2002 Transforming growth factor-beta1 (TGF-beta1) has been shown to stimulate chondrogenesis in periosteal explants cultured in agarose suspension. Sepharose 123-130 transforming growth factor beta 1 Homo sapiens 34-43 12218291-5 2002 We have used a novel immunosorbent consisting of synthetic TFalpha disaccharides (Galbeta1-3GalNAcalpha-) coupled to polyacrylamide (PAA), which itself was covalently bound to cross-linked sepharose. Sepharose 189-198 coagulation factor III, tissue factor Homo sapiens 59-66 11821405-6 2002 eIF4G isoforms from human K562 cells were cleaved with recombinant Coxsackievirus 2A protease and the N- terminal domains purified by m(7)GTP-Sepharose chromatography and polyacrylamide gel electrophoresis. Sepharose 142-151 eukaryotic translation initiation factor 4 gamma 1 Homo sapiens 0-5 11906151-8 2002 The number of c-myc copies/cell was determined with an error rate of less than 10%, where agarose gel bands were stained with ethidium bromide for the product quantitation. Sepharose 90-97 MYC proto-oncogene, bHLH transcription factor Homo sapiens 14-19 11916981-3 2002 Both tumor susceptibility gene 101 (TSG101)/human VPS (hVPS)28 and hepatocyte growth factor receptor substrate (Hrs) cytosolic complexes bind ubiquitin-agarose. Sepharose 152-159 tumor susceptibility 101 Homo sapiens 5-34 11880117-3 2002 Plasma lipids of pre- and post-LDL-apheresis were measured and apolipoprotein E (apoE) localization of the pre- and post-LDL-apheresis was detected by agarose gel electrophoresis. Sepharose 151-158 apolipoprotein E Homo sapiens 63-79 11880117-3 2002 Plasma lipids of pre- and post-LDL-apheresis were measured and apolipoprotein E (apoE) localization of the pre- and post-LDL-apheresis was detected by agarose gel electrophoresis. Sepharose 151-158 apolipoprotein E Homo sapiens 81-85 11880117-8 2002 Plasma apolipoprotein E detected between the prebeta- and alpha-mobility was markedly lower after the LDL-apheresis in the agarose gel electrophoresis. Sepharose 123-130 apolipoprotein E Homo sapiens 7-23 22896898-1 2002 Limited proteolysis of buffalo plasma fibronectin (FN) by thermolysin yielded four gelatin-binding fragments of which, the major 59 kDa fragment, GBF1, was isolated by gelatin-Sepharose and heparin-Sepharose affinity columns. Sepharose 176-185 fibronectin 1 Homo sapiens 51-53 22896898-1 2002 Limited proteolysis of buffalo plasma fibronectin (FN) by thermolysin yielded four gelatin-binding fragments of which, the major 59 kDa fragment, GBF1, was isolated by gelatin-Sepharose and heparin-Sepharose affinity columns. Sepharose 176-185 golgi brefeldin A resistant guanine nucleotide exchange factor 1 Homo sapiens 146-150 22896898-1 2002 Limited proteolysis of buffalo plasma fibronectin (FN) by thermolysin yielded four gelatin-binding fragments of which, the major 59 kDa fragment, GBF1, was isolated by gelatin-Sepharose and heparin-Sepharose affinity columns. Sepharose 198-207 fibronectin 1 Homo sapiens 51-53 22896898-1 2002 Limited proteolysis of buffalo plasma fibronectin (FN) by thermolysin yielded four gelatin-binding fragments of which, the major 59 kDa fragment, GBF1, was isolated by gelatin-Sepharose and heparin-Sepharose affinity columns. Sepharose 198-207 golgi brefeldin A resistant guanine nucleotide exchange factor 1 Homo sapiens 146-150 11916981-3 2002 Both tumor susceptibility gene 101 (TSG101)/human VPS (hVPS)28 and hepatocyte growth factor receptor substrate (Hrs) cytosolic complexes bind ubiquitin-agarose. Sepharose 152-159 tumor susceptibility 101 Homo sapiens 36-42 11916981-3 2002 Both tumor susceptibility gene 101 (TSG101)/human VPS (hVPS)28 and hepatocyte growth factor receptor substrate (Hrs) cytosolic complexes bind ubiquitin-agarose. Sepharose 152-159 MET proto-oncogene, receptor tyrosine kinase Homo sapiens 67-100 11779865-3 2002 The endosomal activity was shown to be aspartic acid protease cathepsin D (CD), based on biochemical similarities to purified CD in 1) the rate and site of substrate cleavage, 2) pH optimum, 3) sensitivity to pepstatin A, and 4) binding to pepstatin A-agarose. Sepharose 252-259 cathepsin D Rattus norvegicus 62-73 11923376-6 2002 Agarose gel electrophoresis of the PstI digests of the PCR products from strains 16M and the vaccine strain Rev.1 revealed a distinctive profile that included three bands: one band for the intact 282-bp fragment amplified from omp2a and two bands resulting from the digestion of the amplified omp2b gene fragment, 238- and 44-bp DNA fragments, respectively. Sepharose 0-7 serine peptidase inhibitor Kazal type 1 Homo sapiens 35-39 11923376-6 2002 Agarose gel electrophoresis of the PstI digests of the PCR products from strains 16M and the vaccine strain Rev.1 revealed a distinctive profile that included three bands: one band for the intact 282-bp fragment amplified from omp2a and two bands resulting from the digestion of the amplified omp2b gene fragment, 238- and 44-bp DNA fragments, respectively. Sepharose 0-7 REV1 DNA directed polymerase Homo sapiens 108-113 11992238-12 2002 The analysis of vWF multimers in the different fractions obtained by affinity chromatography on heparin Sepharose showed that the activity measured both with RCo assay and CBA correlated with the degree of multimerization. Sepharose 104-113 von Willebrand factor Homo sapiens 16-19 12058555-3 2002 The high level of transient expression of GPC recombinant forms in COS 7 cells allowed their purification on Ni-NTA-agarose. Sepharose 116-123 glycophorin-C Cricetulus griseus 42-45 12297026-5 2002 We found that there is a strong correlation between the in vivo CETP inhibition effects and alterations of lipoprotein particle size distribution in rabbit plasma, as determined on an agarose gel electrophoresis and gel filtration column chromatography. Sepharose 184-191 cholesteryl ester transfer protein Oryctolagus cuniculus 64-68 11897038-8 2002 The inhibition of virus production obtained with serum containing anti-HIV-1 IgE was reversed when (1) serum was depleted of IgE (immunoaffinity), but not when it was depleted of IgG (protein G-Sepharose) before inclusion in culture postinfection, (2) anti-IgE, but not anti-IgG, was included in culture, or (3) serum was heat treated before culture. Sepharose 194-203 immunoglobulin heavy constant epsilon Homo sapiens 77-80 11909537-3 2002 In this system, Sepharose beads coated with Ran induce the formation of functional NEs in the absence of chromatin. Sepharose 16-25 ran GTP-binding protein Xenopus laevis 44-47 11779865-3 2002 The endosomal activity was shown to be aspartic acid protease cathepsin D (CD), based on biochemical similarities to purified CD in 1) the rate and site of substrate cleavage, 2) pH optimum, 3) sensitivity to pepstatin A, and 4) binding to pepstatin A-agarose. Sepharose 252-259 cathepsin D Rattus norvegicus 75-77 11870229-1 2002 Human seminal plasma alpha-L-fucosidase (EC 3.2.1.51) has been purified 7100-fold to very high purity and specific activity (83,000 nmol/min/mg protein) by affinity chromatography on agarose-epsilon-aminocaproyl-fucopyranosylamine. Sepharose 183-190 alpha-L-fucosidase 2 Homo sapiens 14-39 11920876-4 2002 A single pI isomer of the labeled Fab" was purified by IEF in a slab of agarose gel and was then used as the affinity probe for alpha(1)-antitrypsin. Sepharose 72-79 FA complementation group B Homo sapiens 34-37 11869079-19 2002 Chondrocytes from normal human cartilage, when cultured in gelled agarose, showed correlations between the expression of TGF-betaRII/TGF-beta1 and the intracellular levels of TIMPs, indicating that TGF-beta autocrine pathway may contribute to homeostasis of the ECM in the normal cartilage. Sepharose 66-73 transforming growth factor beta receptor 2 Homo sapiens 121-132 11811958-6 2002 Direct binding with 32P-labeled tuberin demonstrated Ca2+-dependent binding to CaM-Sepharose which was lost upon deletion of the C-terminal 72 residues. Sepharose 83-92 TSC complex subunit 2 Homo sapiens 32-39 11811958-6 2002 Direct binding with 32P-labeled tuberin demonstrated Ca2+-dependent binding to CaM-Sepharose which was lost upon deletion of the C-terminal 72 residues. Sepharose 83-92 calmodulin 1 Homo sapiens 79-82 11869079-19 2002 Chondrocytes from normal human cartilage, when cultured in gelled agarose, showed correlations between the expression of TGF-betaRII/TGF-beta1 and the intracellular levels of TIMPs, indicating that TGF-beta autocrine pathway may contribute to homeostasis of the ECM in the normal cartilage. Sepharose 66-73 transforming growth factor beta 1 Homo sapiens 121-129 11910554-4 2002 Genotyping of polymorphic APOE alleles was done after polymerase chain reaction amplification of genomic DNA, digestion with HhaI, and agarose gel electrophoresis. Sepharose 135-142 apolipoprotein E Homo sapiens 26-30 11834130-2 2002 For comparison, cell binding to commercially obtained SpA-Sepharose was determined. Sepharose 58-67 surfactant protein A1 Homo sapiens 54-57 11818241-3 2002 Prorenin free of renin was obtained after (NH4)2SO4 precipitation, gel filtration, and ion-exchange chromatography by a passage through an affinity gel of H-77 Sepharose. Sepharose 160-169 renin Rattus norvegicus 3-8 11836176-0 2002 Immunostaining of von Willebrand factor multimers on agarose gels and nitrocellulose filters. Sepharose 53-60 von Willebrand factor Homo sapiens 18-39 11836176-1 2002 Human von Willebrand factor (VWF) multimeric analysis is commonly performed by agarose gel electrophoresis, electroblotting, and immunoenzymatic staining; however, high molecular weight (HMW) multimers are poorly transferred on nitrocellulose and should be visualized by direct gel staining with radiolabeled anti-VWF antibody and autoradiography or luminography. Sepharose 79-86 von Willebrand factor Homo sapiens 6-27 11836176-1 2002 Human von Willebrand factor (VWF) multimeric analysis is commonly performed by agarose gel electrophoresis, electroblotting, and immunoenzymatic staining; however, high molecular weight (HMW) multimers are poorly transferred on nitrocellulose and should be visualized by direct gel staining with radiolabeled anti-VWF antibody and autoradiography or luminography. Sepharose 79-86 von Willebrand factor Homo sapiens 29-32 11860014-3 2002 The proteinase was purified to near homogeneity by ion exchange and affinity chromatography on pepstatin agarose. Sepharose 105-112 endogenous retrovirus group K member 25 Homo sapiens 4-14 12096134-7 2002 Enrichment of target peptides prior to mass spectral analyses is achieved using c-myc-specific antibodies immobilized on protein G-Sepharose beads and facilitates the identification of antigenic peptides spiked at a level of 20 ng/ml in human plasma. Sepharose 131-140 MYC proto-oncogene, bHLH transcription factor Homo sapiens 80-85 11700305-8 2002 When leukocyte extracts were subjected to affinity chromatography on agarose to which residues 751-761 of the CD18 chain phosphorylated at Thr-758 were bound covalently, the only proteins that bound specifically were identified as isoforms of 14-3-3 proteins. Sepharose 69-76 integrin subunit beta 2 Homo sapiens 110-114 11785967-2 2002 The insulin derivative was prepared by treatment of insulin-Sepharose with ammonium bicarbonate. Sepharose 60-69 insulin Homo sapiens 4-11 11785967-2 2002 The insulin derivative was prepared by treatment of insulin-Sepharose with ammonium bicarbonate. Sepharose 60-69 insulin Homo sapiens 52-59 11687588-4 2002 Upon gel-filtration chromatography on Sepharose 4B, partially purified NDH II resolved into two distinct peaks. Sepharose 38-47 DExH-box helicase 9 Homo sapiens 71-77 11687588-5 2002 The first NDH II peak, corresponding to the void volume of Sepharose 4B, displayed coelution with an abundant 42-kDa protein that was subsequently identified as actin. Sepharose 59-68 DExH-box helicase 9 Homo sapiens 10-16 11687588-8 2002 NDH II and hnRNP C from the HeLa nuclear extract coeluted with F-actin on Sepharose 4B in an RNase-resistant manner, whereas hnRNP A1 was nearly completely removed from F-actin-associated hnRNP complexes following RNA digestion. Sepharose 74-83 DExH-box helicase 9 Homo sapiens 0-6 11687588-8 2002 NDH II and hnRNP C from the HeLa nuclear extract coeluted with F-actin on Sepharose 4B in an RNase-resistant manner, whereas hnRNP A1 was nearly completely removed from F-actin-associated hnRNP complexes following RNA digestion. Sepharose 74-83 heterogeneous nuclear ribonucleoprotein C Homo sapiens 11-18 11687588-8 2002 NDH II and hnRNP C from the HeLa nuclear extract coeluted with F-actin on Sepharose 4B in an RNase-resistant manner, whereas hnRNP A1 was nearly completely removed from F-actin-associated hnRNP complexes following RNA digestion. Sepharose 74-83 heterogeneous nuclear ribonucleoprotein C Homo sapiens 11-16 11886166-4 2002 We show that vIL-10 will bind to heparin and use this property to purify vIL-10 from factor Xa cleaved products and trace contaminants using heparin agarose chromatography. Sepharose 149-156 coagulation factor X Homo sapiens 85-94 12144562-5 2002 RESULTS: Among the 13 patients, only 4 had specific IgE to APDH detected by APDH-Sepharose RIA. Sepharose 81-90 immunoglobulin heavy constant epsilon Homo sapiens 52-55 11673471-4 2002 Core 1 beta3-Gal-T activity, which requires Mn2+, was solubilized from rat liver membranes and purified 71,034-fold to apparent homogeneity (>90% purity) in 5.7% yield by ion exchange chromatography on SP-Sepharose, affinity chromatography on immobilized asialo-bovine submaxillary mucin, and gel filtration chromatography on Superose 12. Sepharose 208-217 core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1 Rattus norvegicus 0-18 12144562-4 2002 METHODS: IgE binding of sera from 13 patients allergic to codfish was tested by Sepharose RIA and by Western blot. Sepharose 80-89 immunoglobulin heavy constant epsilon Homo sapiens 9-12 12555814-4 2002 Two higher molecular mass nuclear proteins that are immunoreactive with the sds22 antibodies also copurify on microcystin-Sepharose and may be novel forms of sds22 expressed in mammalian cells. Sepharose 122-131 protein phosphatase 1 regulatory subunit 7 Homo sapiens 76-81 12515911-4 2002 HPF-1 was recovered as a non-adsorbed fraction in blue Sepharose and heparin Sepharose columns, and had a molecular weight of 26-31 kDa as estimated by gel filtration in high salt condition. Sepharose 55-64 histone PARylation factor 1 Rattus norvegicus 0-5 12515911-4 2002 HPF-1 was recovered as a non-adsorbed fraction in blue Sepharose and heparin Sepharose columns, and had a molecular weight of 26-31 kDa as estimated by gel filtration in high salt condition. Sepharose 77-86 histone PARylation factor 1 Rattus norvegicus 0-5 12489150-6 2002 Shh-soaked agarose beads are able to induce the expression of Hip in odontogenic mesenchyme. Sepharose 11-18 sonic hedgehog signaling molecule Homo sapiens 0-3 11882079-1 2002 Extracorporeal immunoadsorption of factor VIII (FVIII) antibodies using Sepharose matrix columns coupled with staphylococcal Protein-A was reported two decades ago. Sepharose 72-81 coagulation factor VIII Homo sapiens 35-46 11882079-1 2002 Extracorporeal immunoadsorption of factor VIII (FVIII) antibodies using Sepharose matrix columns coupled with staphylococcal Protein-A was reported two decades ago. Sepharose 72-81 coagulation factor VIII Homo sapiens 48-53 12803119-6 2002 The bound thrombin was isolated from clot lysate by serial chromatography using a Sepharose 4B column immobilizing an anti-bovine thrombin antibody and a Sepharose 4B column immobilizing an anti-rabbit fibrinogen antibody. Sepharose 82-91 prothrombin Oryctolagus cuniculus 10-18 12803119-6 2002 The bound thrombin was isolated from clot lysate by serial chromatography using a Sepharose 4B column immobilizing an anti-bovine thrombin antibody and a Sepharose 4B column immobilizing an anti-rabbit fibrinogen antibody. Sepharose 82-94 prothrombin Oryctolagus cuniculus 10-18 12227416-6 2002 C-erbB-2 protein, isolated from BT474 cells, a human breast carcinoma cell line with high expression of C-erbB-2 and purified by Concanavalin A-Sepharose 4B affinity chromatography and HPLC has been used to develop the ELISA procedure. Sepharose 144-153 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-8 11853152-1 2002 Lactoferrin with a molecular mass of 80 kDa was purified from equine seminal plasma by heparin-Agarose affinity chromatography and Sephacryl S-200 gel filtration. Sepharose 95-102 inhibitor of carbonic anhydrase Equus caballus 0-11 11848461-3 2002 In the present study, it was found, by means of agarose gel electrophoresis, that the pre-incubation of full-length rTFPI with heparin or the carboxy (C)-terminal part (peptide 240-265) of TFPI prevented the association with ox-LDL in a dose-dependent manner. Sepharose 48-55 tissue factor pathway inhibitor Homo sapiens 117-121 12536519-6 2001 Purification of the GST-fused FBXO30 was carried out by affinity chromatography with glutathione sepharose 4B. Sepharose 97-109 F-box protein 30 Homo sapiens 30-36 12144562-6 2002 The two patients who had the highest level of specific IgE to human APDH also had a class 5-6 CAP-RAST IgE level to codfish, but two other patients with a class 5 had a negative APDH-Sepharose IgE-RIA. Sepharose 183-192 immunoglobulin heavy constant epsilon Homo sapiens 55-58 11736641-3 2001 In the present study, we show: (i) by affinity chromatography on L-selectin-agarose; (ii) by protein identification using MS; and (iii) by covalent cell-surface labelling with sulphosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate that the multifunctional nuclear protein nucleolin is partly exposed on the cell surface, and is a ligand of L-selectin in human leucocytes and haematopoietic progenitor cells. Sepharose 76-83 selectin L Homo sapiens 65-75 11739191-3 2001 The nickel-agarose-purified His-M195FANCF protein bound specifically to the surface of HeLa cells transfected with CD33 and internalized through vesicular structures. Sepharose 11-18 FA complementation group F Homo sapiens 36-41 11739191-3 2001 The nickel-agarose-purified His-M195FANCF protein bound specifically to the surface of HeLa cells transfected with CD33 and internalized through vesicular structures. Sepharose 11-18 CD33 molecule Homo sapiens 115-119 11747299-4 2001 PP1beta9C was purified to a specific activity of 12,077 mU/mg by a three-step purification method comprising (NH(4))(2)SO(4)-ethanol precipitation followed by Ni(2+)-agarose affinity chromatography and Mono Q anion-exchange chromatography. Sepharose 166-173 flapwing Drosophila melanogaster 0-9 11730480-6 2001 IgG and Cat were separated in a second Q-Sepharose chromatography step during which IgG was recovered in the flow-through, whereas Cat bound to the resin. Sepharose 41-50 catalase Homo sapiens 8-11 11730480-8 2001 Cat was eluted at the second Q-Sepharose chromatography step and was purified by Blue Sepharose chromatography. Sepharose 31-40 catalase Homo sapiens 0-3 11730480-8 2001 Cat was eluted at the second Q-Sepharose chromatography step and was purified by Blue Sepharose chromatography. Sepharose 86-95 catalase Homo sapiens 0-3 11730480-10 2001 Sod was further purified by S-Sepharose and Phenyl-Sepharose chromatography steps and recovered in the non-adsorbed fractions. Sepharose 28-39 superoxide dismutase 1 Homo sapiens 0-3 11745155-4 2001 We propose a novel approach to the inhibition of angiogenesis by immobilizing VE-cadherin-secreting hybridoma cells in alginate-agarose microcapsules. Sepharose 128-135 cadherin 5 Mus musculus 78-89 11826961-1 2001 Trehalase (EC 3.2.1.28) of the bound type was purified as an electrophoretically homogeneous protein from adult honeybees by fractionation with ammonium sulfate, hydrophobic chromatography, and DEAE-Sepharose CL-6B, CM-Sepharose CL-6B, butyl-Toyopearl 650M, and p-aminophenyl beta-glucoside Sepharose 4B column chromatographies. Sepharose 199-208 trehalase Apis mellifera 0-9 11826984-5 2001 The inclusion bodies of recombinant OLP were solubilized in 8 M urea and purified on an SP-Sepharose column. Sepharose 91-100 osmotin-like protein Nicotiana tabacum 36-39 11718686-5 2001 Apo(a) K4-VNTR was measured by high-resolution SDS-agarose gel separation and ECL Western blotting method. Sepharose 51-58 lipoprotein(a) Homo sapiens 0-6 11718686-5 2001 Apo(a) K4-VNTR was measured by high-resolution SDS-agarose gel separation and ECL Western blotting method. Sepharose 51-58 keratin 4 Homo sapiens 7-14 11798074-6 2001 The product of polymerase chain reaction (PCR) of ALDH2 exon 12 could be easily screened by electrophoresis on a 2% agarose gel. Sepharose 116-123 aldehyde dehydrogenase 2 family member Homo sapiens 50-55 11707271-2 2001 A cAMP-agarose-based affinity purification procedure yielded three proteins which were identified by mass spectrometry as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and two nucleoside diphosphate kinases (NDPKs). Sepharose 7-14 glyceraldehyde-3-phosphate dehydrogenase, cytosolic Nicotiana tabacum 122-162 11707271-2 2001 A cAMP-agarose-based affinity purification procedure yielded three proteins which were identified by mass spectrometry as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and two nucleoside diphosphate kinases (NDPKs). Sepharose 7-14 glyceraldehyde-3-phosphate dehydrogenase, cytosolic Nicotiana tabacum 164-169 11731083-2 2001 The lectin (CCL) was purified by affinity chromatography on a Lactosyl-Sepharose 4B column. Sepharose 71-80 CCR-like protein Arabidopsis thaliana 12-15 11687285-6 2001 In the converse experiment, alpha-synuclein bound to a column prepared from purified bovine brain tubulin immobilized upon CNBr-Sepharose. Sepharose 128-137 synuclein alpha Bos taurus 28-43 11703381-4 2001 The binding activity of IgG fractions positive for anti-U1-RNP to the endothelial cells could be effectively absorbed by U1-RNP-Sepharose. Sepharose 128-137 small nuclear ribonucleoprotein U1 subunit 70 Homo sapiens 56-62 11703381-4 2001 The binding activity of IgG fractions positive for anti-U1-RNP to the endothelial cells could be effectively absorbed by U1-RNP-Sepharose. Sepharose 128-137 RNA binding region (RNP1, RRM) containing 3 Homo sapiens 59-62 11592051-2 2001 METHODS: DNA was extracted from peripheral blood of HA patients; F VIII gene inversion was identified by long distance-polymerase chain reaction(LD-PCR) and 0.6% agarose gel electrophoresis. Sepharose 162-169 cytochrome c oxidase subunit 8A Homo sapiens 67-71 11673619-7 2001 The addition of membrane-impermeable CaM inhibitors, such as anti-CaM antiserum or W7-agarose, repressed the expression of RbcS-GUS in darkness, but this inhibitory effect was completely reversed by adding exogenous purified CaM. Sepharose 86-93 ribulose bisphosphate carboxylase small chain S41, chloroplastic Nicotiana tabacum 123-127 11778926-3 2001 Today, SPA-based extracorporeal immunoadsorption relies on two rather different systems, namely, SPA-silica (Prosorba), and SPA-Sepharose (Immunosorba). Sepharose 128-137 surfactant protein A1 Homo sapiens 7-10 11778926-4 2001 Both systems are approved by the Food and Drug Administration for the core indications of rheumatoid arthritis and idiopathic thrombocytopenic purpura (SPA-silica) or hemophilia with inhibitors (SPA-Sepharose). Sepharose 199-208 surfactant protein A1 Homo sapiens 195-198 11473115-3 2001 To identify specific lysine residues in the amino terminus of apoB that are required for the noncovalent interaction, we initially used an affinity chromatography method in which recombinant forms of apo(a) (r-apo(a)) were immobilized on Sepharose beads. Sepharose 238-247 apolipoprotein B Homo sapiens 62-66 11473115-4 2001 Assessment of the ability of carboxyl-terminal truncations of apoB-18 to bind to r-apo(a)-Sepharose revealed that a 25-amino acid sequence in apoB (amino acids 680-704) bound specifically to apo(a) in a lysine-dependent manner; citraconylation of the lysine residues in the apoB derivative encoding this sequence abolished the binding interaction. Sepharose 90-99 apolipoprotein B Homo sapiens 62-66 11473115-4 2001 Assessment of the ability of carboxyl-terminal truncations of apoB-18 to bind to r-apo(a)-Sepharose revealed that a 25-amino acid sequence in apoB (amino acids 680-704) bound specifically to apo(a) in a lysine-dependent manner; citraconylation of the lysine residues in the apoB derivative encoding this sequence abolished the binding interaction. Sepharose 90-99 lipoprotein(a) Homo sapiens 83-89 11473115-4 2001 Assessment of the ability of carboxyl-terminal truncations of apoB-18 to bind to r-apo(a)-Sepharose revealed that a 25-amino acid sequence in apoB (amino acids 680-704) bound specifically to apo(a) in a lysine-dependent manner; citraconylation of the lysine residues in the apoB derivative encoding this sequence abolished the binding interaction. Sepharose 90-99 apolipoprotein B Homo sapiens 142-146 11473115-4 2001 Assessment of the ability of carboxyl-terminal truncations of apoB-18 to bind to r-apo(a)-Sepharose revealed that a 25-amino acid sequence in apoB (amino acids 680-704) bound specifically to apo(a) in a lysine-dependent manner; citraconylation of the lysine residues in the apoB derivative encoding this sequence abolished the binding interaction. Sepharose 90-99 apolipoprotein B Homo sapiens 142-146 11598051-10 2001 Whereas cyclin B1 was not detectably affected in E22-exposed cells, Cdk1 was maintained in a tyrosine-phosphorylated inactive state and lost its affinity for p13(suc1)-agarose beads. Sepharose 168-175 cyclin dependent kinase 1 Homo sapiens 68-72 12561612-3 2001 Agarose gel electrophoresis of DNA from the cells treated with LBP-X revealed a "DNA ladder" and positive TUNEL test. Sepharose 0-7 lipopolysaccharide binding protein Homo sapiens 63-66 11694291-1 2001 The preparation, testing and use of a variety of cholesterol-, deoxycorticosterone (DOC)- and pregnenolone-binding 1,6-diaminohexyl (EAH)-Sepharose 4B supports for affinity column chromatography of cytochromes P-450(scc) and P-450(17 alpha) from bovine adrenal and pig testis are described. Sepharose 138-147 SCC Bos taurus 210-220 11694297-2 2001 Alanine methyl ketone-Sepharose (AMK-Sepharose) is introduced as affinity support for the purification of a bacterial alanyl aminopeptidase (AAP) from a membrane protein extract and Arginine-Agarose as support for the preparation of a membrane-bound proteinase of myeloma cells (MP-1). Sepharose 22-31 alanyl aminopeptidase, membrane Homo sapiens 118-139 11694297-2 2001 Alanine methyl ketone-Sepharose (AMK-Sepharose) is introduced as affinity support for the purification of a bacterial alanyl aminopeptidase (AAP) from a membrane protein extract and Arginine-Agarose as support for the preparation of a membrane-bound proteinase of myeloma cells (MP-1). Sepharose 22-31 alanyl aminopeptidase, membrane Homo sapiens 141-144 11694297-2 2001 Alanine methyl ketone-Sepharose (AMK-Sepharose) is introduced as affinity support for the purification of a bacterial alanyl aminopeptidase (AAP) from a membrane protein extract and Arginine-Agarose as support for the preparation of a membrane-bound proteinase of myeloma cells (MP-1). Sepharose 37-46 alanyl aminopeptidase, membrane Homo sapiens 118-139 11694297-2 2001 Alanine methyl ketone-Sepharose (AMK-Sepharose) is introduced as affinity support for the purification of a bacterial alanyl aminopeptidase (AAP) from a membrane protein extract and Arginine-Agarose as support for the preparation of a membrane-bound proteinase of myeloma cells (MP-1). Sepharose 37-46 alanyl aminopeptidase, membrane Homo sapiens 141-144 11563973-7 2001 Agarose-gel electrophoresis of metabolically radiolabelled d-Hml suggested that it is secreted from Kc167 cells mainly as dimers. Sepharose 0-7 Hemolectin Drosophila melanogaster 59-64 11795466-2 2001 Hsp90 was either immunoadsorbed to BF4 antibody-Sepharose or GST-Hsp90 fusion protein (GST.90) was adsorbed to glutathione-Sepharose. Sepharose 48-57 heat shock protein 90 alpha family class A member 1 Homo sapiens 0-5 11695366-9 2001 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of surface biotinylated proteins affinity-purified on lactoferrin-Sepharose showed biotinylated bands at Mr values of 22, 49, 55, 72, and 155 kDa. Sepharose 124-133 lactotransferrin Bos taurus 112-123 11473115-7 2001 We found that the apoB-18 species containing the Lys(680) mutation was incapable of binding to r-apo(a)-Sepharose columns, whereas the apoB-18 species containing the Lys(690) mutation exhibited slightly reduced binding to these columns. Sepharose 104-113 apolipoprotein B Homo sapiens 18-22 11535495-6 2001 In this study, the purification of human vWF-cleaving protease from a commercial preparation of factor VIII/vWF concentrate by means of several column chromatographic steps, including 2 steps of heparin-Sepharose column, is reported. Sepharose 203-212 ADAM metallopeptidase with thrombospondin type 1 motif 13 Homo sapiens 41-62 11535495-6 2001 In this study, the purification of human vWF-cleaving protease from a commercial preparation of factor VIII/vWF concentrate by means of several column chromatographic steps, including 2 steps of heparin-Sepharose column, is reported. Sepharose 203-212 von Willebrand factor Homo sapiens 41-44 11748924-8 2001 On the other hand, the leptin-stimulated tyrosine phosphorylation of Sam68 mediates the dissociation from RNA as assessed by Sepharose-conjugated poly(U) binding. Sepharose 125-134 leptin Homo sapiens 23-29 11748924-8 2001 On the other hand, the leptin-stimulated tyrosine phosphorylation of Sam68 mediates the dissociation from RNA as assessed by Sepharose-conjugated poly(U) binding. Sepharose 125-134 KH RNA binding domain containing, signal transduction associated 1 Homo sapiens 69-74