PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
13680162-6	2003	The DPD and TS activities were quantified by a radiometric enzymatic assay and a 5-fluoro-2"-deoxyuridine-5"- monophosphate (FdUMP) ligand-binding assay, respectively.	Fluorodeoxyuridylate	81-123	dihydropyrimidine dehydrogenase	Homo sapiens	4-7
15930305-1	2005	FdUMP[10], a 10mer of 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP), the thymidylate synthase inhibitory metabolite of 5-fluorouracil (FU), is most closely correlated with the DNA topoisomerase I (Top1) inhibitor camptothecin in the National Cancer Institute COMPARE analysis, but not with FU.	Fluorodeoxyuridylate	0-5	topoisomerase (DNA) I	Mus musculus	180-199
15930305-1	2005	FdUMP[10], a 10mer of 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP), the thymidylate synthase inhibitory metabolite of 5-fluorouracil (FU), is most closely correlated with the DNA topoisomerase I (Top1) inhibitor camptothecin in the National Cancer Institute COMPARE analysis, but not with FU.	Fluorodeoxyuridylate	22-63	thymidylate synthase	Mus musculus	77-97
15930305-1	2005	FdUMP[10], a 10mer of 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP), the thymidylate synthase inhibitory metabolite of 5-fluorouracil (FU), is most closely correlated with the DNA topoisomerase I (Top1) inhibitor camptothecin in the National Cancer Institute COMPARE analysis, but not with FU.	Fluorodeoxyuridylate	22-63	topoisomerase (DNA) I	Mus musculus	180-199
14770427-2	2004	5-FU is a prodrug, and orotate phosphoribosyltransferase (OPRT) is the principal enzyme that converts 5-FU directly into an active antitumor metabolite, 5-fluoro-2"-deoxyuridine 5"-monophosphate.	Fluorodeoxyuridylate	153-194	uridine monophosphate synthetase	Homo sapiens	23-56
14770427-2	2004	5-FU is a prodrug, and orotate phosphoribosyltransferase (OPRT) is the principal enzyme that converts 5-FU directly into an active antitumor metabolite, 5-fluoro-2"-deoxyuridine 5"-monophosphate.	Fluorodeoxyuridylate	153-194	uridine monophosphate synthetase	Homo sapiens	58-62
14713770-2	2004	It is a prodrug and orotate phosphoribosyltransferase (OPRT) is the principal enzyme that directly converts 5-FU to the active anticancer metabolite 5-fluoro-2"-deoxyuridine 5"-monophosphate.	Fluorodeoxyuridylate	149-190	uridine monophosphate synthetase	Homo sapiens	20-53
14713770-2	2004	It is a prodrug and orotate phosphoribosyltransferase (OPRT) is the principal enzyme that directly converts 5-FU to the active anticancer metabolite 5-fluoro-2"-deoxyuridine 5"-monophosphate.	Fluorodeoxyuridylate	149-190	uridine monophosphate synthetase	Homo sapiens	55-59
16505899-1	2006	Thymidylate synthase purified from 5-fluoro-dUrd-resistant mouse leukemia L1210 cells (TSr) was less sensitive to slow-binding inhibition by 5-fluoro-dUMP than the enzyme from the parental cells (TSp), both enzyme forms differing also in sensitivity to several other dump analogues, apparent molecular weights of monomer and dimer, and temperature dependence of the catalyzed reaction.	Fluorodeoxyuridylate	141-154	thymidylate synthase	Mus musculus	0-20
13680162-6	2003	The DPD and TS activities were quantified by a radiometric enzymatic assay and a 5-fluoro-2"-deoxyuridine-5"- monophosphate (FdUMP) ligand-binding assay, respectively.	Fluorodeoxyuridylate	81-123	thymidylate synthetase	Homo sapiens	12-14
12167486-1	2002	Folate based inhibitors of thymidylate synthase (TS) might facilitate binding of 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) to TS similar to the natural reduced folate 5,10-methylenetetrahydrofolate (CH(2)-H(4)-folate).	Fluorodeoxyuridylate	81-122	thymidylate synthetase	Homo sapiens	27-47
12929570-6	2003	After infusion of 5-FU (40 mg/kg body), the serum 5-FU concentration and 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP)-bound TS levels in the jejunum were significantly higher in the PN group than the FED group (156.8 +/- 51.9 vs 100.5 +/- 51.9 ng/ml, p < 0.001 and 38.55 +/- 7.61 vs 22.89 +/- 4.46 pmol/g of tissue, p < 0.01, respectively).	Fluorodeoxyuridylate	73-114	thymidylate synthetase	Rattus norvegicus	129-131
12684419-7	2003	EXPERIMENTAL DESIGN: The levels of TS and DPD activities in nonfixed fresh-frozen RCC and normal kidney were determined biochemically by the 5-fluoro-2"-deoxyuridine 5"-monophosphate binding assay and the 5-FU degradation assay, respectively.	Fluorodeoxyuridylate	141-182	thymidylate synthetase	Homo sapiens	35-37
12820419-3	2003	This product can form 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP) through the action of thymidine kinase, and FdUMP in turn can inhibit thymidylate synthase (TS), leading to reduced thymidylate formation and subsequent inhibition of DNA synthesis.	Fluorodeoxyuridylate	22-63	thymidylate synthetase	Homo sapiens	142-162
12820419-3	2003	This product can form 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP) through the action of thymidine kinase, and FdUMP in turn can inhibit thymidylate synthase (TS), leading to reduced thymidylate formation and subsequent inhibition of DNA synthesis.	Fluorodeoxyuridylate	22-63	thymidylate synthetase	Homo sapiens	164-166
12553027-5	2002	Thymidine phosphorylase (TP), the enzyme that converts 5"-DFUR into 5-FU and 5-FU into 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP), was estimated by ELISA.	Fluorodeoxyuridylate	87-128	thymidine phosphorylase	Homo sapiens	0-23
12553027-5	2002	Thymidine phosphorylase (TP), the enzyme that converts 5"-DFUR into 5-FU and 5-FU into 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP), was estimated by ELISA.	Fluorodeoxyuridylate	87-128	thymidine phosphorylase	Homo sapiens	25-27
12553027-5	2002	Thymidine phosphorylase (TP), the enzyme that converts 5"-DFUR into 5-FU and 5-FU into 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP), was estimated by ELISA.	Fluorodeoxyuridylate	130-135	thymidine phosphorylase	Homo sapiens	0-23
12553027-5	2002	Thymidine phosphorylase (TP), the enzyme that converts 5"-DFUR into 5-FU and 5-FU into 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP), was estimated by ELISA.	Fluorodeoxyuridylate	130-135	thymidine phosphorylase	Homo sapiens	25-27
12883718-2	2003	Thymidylate synthase (TS) is inhibited to form an inactive ternary complex by 5-fluoro-dUMP and is considered to be a target enzyme of 5-FU treatment.	Fluorodeoxyuridylate	78-91	thymidylate synthetase	Homo sapiens	0-20
12883718-2	2003	Thymidylate synthase (TS) is inhibited to form an inactive ternary complex by 5-fluoro-dUMP and is considered to be a target enzyme of 5-FU treatment.	Fluorodeoxyuridylate	78-91	thymidylate synthetase	Homo sapiens	22-24
12167486-1	2002	Folate based inhibitors of thymidylate synthase (TS) might facilitate binding of 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) to TS similar to the natural reduced folate 5,10-methylenetetrahydrofolate (CH(2)-H(4)-folate).	Fluorodeoxyuridylate	124-129	thymidylate synthetase	Homo sapiens	27-47
12084461-2	2002	TS is an important target for chemotherapy; it is inhibited by folate and nucleotide analogs, such as by 5-fluoro-dUMP (FdUMP), the active metabolite of 5-fluorouracil (5FU).	Fluorodeoxyuridylate	105-118	thymidylate synthetase	Homo sapiens	0-2
11836594-5	2002	The levels of TS and DPD activities in non-fixed fresh frozen bladder cancer specimens were determined biochemically by the 5-fluoro-2"-deoxyuridine 5"-monophosphate binding assay and the 5-fluorouracil degradation assay, respectively.	Fluorodeoxyuridylate	124-165	thymidylate synthetase	Homo sapiens	14-16
11750847-15	2002	Incubation of Caki-1 cells with TRAIL enhanced the intracellular accumulation of 5-FU and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	90-131	TNF superfamily member 10	Homo sapiens	32-37
11750847-15	2002	Incubation of Caki-1 cells with TRAIL enhanced the intracellular accumulation of 5-FU and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	133-138	TNF superfamily member 10	Homo sapiens	32-37
10741735-8	2000	A significant correlation was found between TP enzyme activity and 5-fluoro-2"-deoxyuridine-5"-monophosphate binding activity.	Fluorodeoxyuridylate	67-108	thymidine phosphorylase	Homo sapiens	44-46
11101356-7	2000	Thymidylate synthase inhibition was studied with two forms of the enzyme differing in sensitivities toward 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP), isolated from parental and FdUrd-resistant L1210 cell lines.	Fluorodeoxyuridylate	107-148	thymidylate synthase	Mus musculus	0-20
11212266-3	2001	In the cell, 5-FU is metabolized to 5-fluoro-2"-deoxyuridine 5"-monophosphate, a tight binding covalent inhibitor of thymidylate synthase.	Fluorodeoxyuridylate	36-77	thymidylate synthetase	Homo sapiens	117-137
10509749-8	1999	FdUrd is a prodrug of the tight-binding TS inhibitor, 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	54-95	thymidylate synthetase	Homo sapiens	40-42
10535743-5	1999	The levels of functional TS in C18 cells were analyzed by the binding of the mechanism-based inhibitor 5-fluoro-2"-deoxyuridylate (FdUMP) under conditions that allowed for the detection of 10 fmol of TS.	Fluorodeoxyuridylate	131-136	thymidylate synthetase	Homo sapiens	25-27
10697629-2	1999	5-FU is metabolically converted to 5-fluorouracil-2"-deoxyuridine-5"-monophosphate-(FdUMP) which is believed to inhibit DNA synthesis in neoplastic cells by forming a tightly bound ternary complex with thymidylate synthase (TS).	Fluorodeoxyuridylate	84-89	thymidylate synthetase	Homo sapiens	202-222
10697629-2	1999	5-FU is metabolically converted to 5-fluorouracil-2"-deoxyuridine-5"-monophosphate-(FdUMP) which is believed to inhibit DNA synthesis in neoplastic cells by forming a tightly bound ternary complex with thymidylate synthase (TS).	Fluorodeoxyuridylate	84-89	thymidylate synthetase	Homo sapiens	224-226
10509749-8	1999	FdUrd is a prodrug of the tight-binding TS inhibitor, 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	97-102	thymidylate synthetase	Homo sapiens	40-42
10441376-1	1999	A naturally occurring mutant of human thymidylate synthase (hTS) that contains a Tyr to His mutation at residue 33 was found to confer 4-fold resistance to 5-fluoro-2"-deoxyuridine (FdUrd), a prodrug of 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	203-244	thymidylate synthetase	Homo sapiens	38-58
10441376-1	1999	A naturally occurring mutant of human thymidylate synthase (hTS) that contains a Tyr to His mutation at residue 33 was found to confer 4-fold resistance to 5-fluoro-2"-deoxyuridine (FdUrd), a prodrug of 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	203-244	APC down-regulated 1	Homo sapiens	60-63
10441376-1	1999	A naturally occurring mutant of human thymidylate synthase (hTS) that contains a Tyr to His mutation at residue 33 was found to confer 4-fold resistance to 5-fluoro-2"-deoxyuridine (FdUrd), a prodrug of 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	246-251	APC down-regulated 1	Homo sapiens	60-63
10441376-1	1999	A naturally occurring mutant of human thymidylate synthase (hTS) that contains a Tyr to His mutation at residue 33 was found to confer 4-fold resistance to 5-fluoro-2"-deoxyuridine (FdUrd), a prodrug of 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	246-251	thymidylate synthetase	Homo sapiens	38-58
10652619-2	1999	We investigated the polymorphism on the repeat length of the TS gene and its relation to the number of 5-fluoro-dUMP (FdUMP) binding sites in human gastrointestinal cancers.	Fluorodeoxyuridylate	103-116	thymidylate synthetase	Homo sapiens	61-63
9186496-1	1997	Fluorinated pyrimidines, such as 5-fluorouracil (FUra) and 5-fluoro-2"-deoxyuridine (FdUrd), are cytotoxic to cells as a consequence of generation of 5-fluoro-2"-deoxyuridylate (FdUMP), which is a mechanism-based inhibitor of the enzyme thymidylate synthase (TS).	Fluorodeoxyuridylate	178-183	thymidylate synthetase	Homo sapiens	237-257
10212232-4	1999	The fluoropyrimidines 5-fluorouracil and 5-fluoro-2"-deoxyuridine are cytotoxic as a consequence of inhibition of TS by the metabolite 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	135-176	thymidylate synthetase	Homo sapiens	114-116
10212232-4	1999	The fluoropyrimidines 5-fluorouracil and 5-fluoro-2"-deoxyuridine are cytotoxic as a consequence of inhibition of TS by the metabolite 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	178-183	thymidylate synthetase	Homo sapiens	114-116
9565579-9	1998	We postulate that the D49G or G52S mutation leads to the structural perturbation of the highly conserved Arg50 loop, decreasing the binding of thymidylate synthase to the inhibitors, Thymitaq and fluorodeoxyuridylate.	Fluorodeoxyuridylate	196-216	thymidylate synthetase	Homo sapiens	143-163
9701498-0	1998	Acyclic analogues of 5-fluoro-dUMP and 5-fluoro-2"-deoxyuridine: synthesis and inhibition of thymidylate synthase and tumour cell growth.	Fluorodeoxyuridylate	21-34	thymidylate synthase	Mus musculus	93-113
9535167-4	1997	Included among these structures are complexes of TS bound to substrate dUMP; cofactor CH2THF; the nucleotide analogs 5-fluoro-dUMP, 5-nitro-dUMP and dGMP; and the promising antifolates BW1843, ZD1694, and AG337.	Fluorodeoxyuridylate	117-130	thymidylate synthetase	Homo sapiens	49-51
9186496-1	1997	Fluorinated pyrimidines, such as 5-fluorouracil (FUra) and 5-fluoro-2"-deoxyuridine (FdUrd), are cytotoxic to cells as a consequence of generation of 5-fluoro-2"-deoxyuridylate (FdUMP), which is a mechanism-based inhibitor of the enzyme thymidylate synthase (TS).	Fluorodeoxyuridylate	178-183	thymidylate synthetase	Homo sapiens	259-261
9148948-2	1997	In the latter case, the location of TS was established through the use of [6-3H]5-fluorodeoxyuridine, which forms a tight ternary complex of TS with 5-fluorodeoxyuridylate (FdUMP) and 5, 10-methylenetetrahydrofolylpolyglutamate within the cell.	Fluorodeoxyuridylate	173-178	thymidylate synthetase	Rattus norvegicus	36-38
9148948-2	1997	In the latter case, the location of TS was established through the use of [6-3H]5-fluorodeoxyuridine, which forms a tight ternary complex of TS with 5-fluorodeoxyuridylate (FdUMP) and 5, 10-methylenetetrahydrofolylpolyglutamate within the cell.	Fluorodeoxyuridylate	173-178	thymidylate synthetase	Rattus norvegicus	141-143
8632414-1	1996	To develop novel lipophilic thymidylate synthase (TS) inhibitors, the X-ray structure of Escherichia coli TS in ternary complex with FdUMP and the inhibitor 10-propargyl-5,8-dideazafolic acid (CB3717) was used as a basis for structure-based design.	Fluorodeoxyuridylate	133-138	thymidylate synthetase	Homo sapiens	28-48
9155534-3	1997	IFN-alpha may elevate the levels of the active 5-FU metabolite 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) in the cell, possibly leading to increased inhibition of the target enzyme thymidylate synthase (TS), which might enhance DNA damage.	Fluorodeoxyuridylate	63-104	interferon alpha 1	Homo sapiens	0-9
9155534-3	1997	IFN-alpha may elevate the levels of the active 5-FU metabolite 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) in the cell, possibly leading to increased inhibition of the target enzyme thymidylate synthase (TS), which might enhance DNA damage.	Fluorodeoxyuridylate	63-104	thymidylate synthetase	Homo sapiens	188-208
9155534-3	1997	IFN-alpha may elevate the levels of the active 5-FU metabolite 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) in the cell, possibly leading to increased inhibition of the target enzyme thymidylate synthase (TS), which might enhance DNA damage.	Fluorodeoxyuridylate	106-111	interferon alpha 1	Homo sapiens	0-9
9155534-3	1997	IFN-alpha may elevate the levels of the active 5-FU metabolite 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) in the cell, possibly leading to increased inhibition of the target enzyme thymidylate synthase (TS), which might enhance DNA damage.	Fluorodeoxyuridylate	106-111	thymidylate synthetase	Homo sapiens	188-208
8790719-0	1996	Molecular mechanism of thymidylate synthase-catalyzed reaction and interaction of the enzyme with 2- and/or 4-substituted analogues of dUMP and 5-fluoro-dUMP.	Fluorodeoxyuridylate	144-157	thymidylate synthetase	Homo sapiens	23-43
7627962-6	1995	One of the compounds, the novel pyrimidine analogue 5-ethoxy-2"-deoxyuridine (EOdU), was found to be a substrate for the transferase reaction of TP, to have little or no direct cytotoxicity, to selectively increase the cellular levels of 5-fluoro-dUMP, to enhance the inhibitory effect of FUra on thymidylate synthase activity, and to potentiate the cytotoxicity of FUra and IFN in human colon carcinoma cells.	Fluorodeoxyuridylate	238-251	thymidine phosphorylase	Homo sapiens	145-147
8823494-1	1996	Interferon (IFN) augments the anabolism of 5-fluorouracil (5FU) to its active metabolite, fluorodeoxyuridylate (FdUMP), which inhibits thymidylate synthase (TS).	Fluorodeoxyuridylate	90-110	interferon alpha 1	Homo sapiens	0-10
8823494-1	1996	Interferon (IFN) augments the anabolism of 5-fluorouracil (5FU) to its active metabolite, fluorodeoxyuridylate (FdUMP), which inhibits thymidylate synthase (TS).	Fluorodeoxyuridylate	90-110	interferon alpha 1	Homo sapiens	12-15
8823494-1	1996	Interferon (IFN) augments the anabolism of 5-fluorouracil (5FU) to its active metabolite, fluorodeoxyuridylate (FdUMP), which inhibits thymidylate synthase (TS).	Fluorodeoxyuridylate	90-110	thymidylate synthetase	Homo sapiens	135-155
8790719-2	1996	With two dUMP analogues, 5-fluoro-dUMP (FdUMP) and 5-(trifluoromethyl)-dUMP (CF3dUMP), strong thymidylate synthase inhibitors and active forms of drugs, the inhibition mechanism is based on the reaction mechanism.	Fluorodeoxyuridylate	25-38	thymidylate synthetase	Homo sapiens	94-114
7641209-1	1995	Thymidylate synthase (TS) is a target enzyme of 5-fluorouracil and is inhibited by 5-fluoro-dUMP (FdUMP) to form an inactive ternary complex.	Fluorodeoxyuridylate	83-96	thymidylate synthetase	Homo sapiens	0-20
7641209-1	1995	Thymidylate synthase (TS) is a target enzyme of 5-fluorouracil and is inhibited by 5-fluoro-dUMP (FdUMP) to form an inactive ternary complex.	Fluorodeoxyuridylate	83-96	thymidylate synthetase	Homo sapiens	22-24
8461049-0	1993	Effect of folate diastereoisomers on the binding of 5-fluoro-2"-deoxyuridine-5"-monophosphate to thymidylate synthase.	Fluorodeoxyuridylate	52-93	thymidylate synthetase	Homo sapiens	97-117
8407987-4	1993	Analysis of the in vitro translation product of FUra-substituted TS mRNA by Western immunoblotting, isoelectric focusing, 5-fluoro-2"-deoxyuridine 5"-monophosphate binding, and TS catalytic activity experiments shows no difference compared to control TS mRNA.	Fluorodeoxyuridylate	122-163	thymidylate synthase	Oryctolagus cuniculus	65-67
8355668-1	1993	A major mechanism underlying the cytotoxicity of fluoropyrimidine analogs such as 5-fluorouracil and 5-fluoro-2"-deoxyuridine (FdUrd) occurs via the formation of 5-fluoro-2"-deoxyuridylate (FdUMP), a tight-binding inhibitor of thymidylate synthase (TS).	Fluorodeoxyuridylate	190-195	thymidylate synthetase	Homo sapiens	227-247
7763285-4	1995	Using both the radioenzymatic 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) binding and catalytic assays for measurement of thymidylate synthase (TS) enzyme activity, there was significantly increased TS activity in resistant H630-R1 (13- and 23-fold), H630-R10 (37- and 40-fold), and H630-R (24- and 34-fold) lines, for binding and catalytic assays, respectively, compared with the parent H630 line.	Fluorodeoxyuridylate	30-71	thymidylate synthetase	Homo sapiens	205-207
8500219-2	1993	The reduced folate 5,10-methylenetetrahydrofolate (CH2FH4) and its precursor tetrahydrofolate (FH4) are essential cofactors for the formation of a tight ternary complex of thymidylate synthase (TS) and 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) derived from FUra.	Fluorodeoxyuridylate	202-243	thymidylate synthetase	Rattus norvegicus	172-192
8500219-2	1993	The reduced folate 5,10-methylenetetrahydrofolate (CH2FH4) and its precursor tetrahydrofolate (FH4) are essential cofactors for the formation of a tight ternary complex of thymidylate synthase (TS) and 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) derived from FUra.	Fluorodeoxyuridylate	245-250	thymidylate synthetase	Rattus norvegicus	172-192
1532673-6	1992	The resistant cells showed significantly increased levels of thymidylate synthase, the target enzyme of the fluoropyrimidines" active metabolite, 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	146-187	thymidylate synthetase	Homo sapiens	61-81
1532344-3	1992	This acyclonucleoside analogue is an inhibitor of thymidylate synthase (TS) as determined by inhibition of [6-3H]-2"-deoxyuridine incorporation into DNA, inhibition of 3H release from [5-3H]-2"-deoxyuridine, and decrease in both the free and total TS 5"-fluoro-2"-deoxyuridine 5"-monophosphate binding sites.	Fluorodeoxyuridylate	251-293	thymidylate synthetase	Homo sapiens	50-70
1557655-1	1992	The inhibition of thymidylate synthase (TS) by the fluorouracil (5-FU) metabolite 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) is considered to be one of the main mechanisms of action of 5-FU.	Fluorodeoxyuridylate	82-123	thymidylate synthetase	Homo sapiens	18-38
1532673-6	1992	The resistant cells showed significantly increased levels of thymidylate synthase, the target enzyme of the fluoropyrimidines" active metabolite, 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	189-194	thymidylate synthetase	Homo sapiens	61-81
1557655-1	1992	The inhibition of thymidylate synthase (TS) by the fluorouracil (5-FU) metabolite 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) is considered to be one of the main mechanisms of action of 5-FU.	Fluorodeoxyuridylate	82-123	thymidylate synthetase	Homo sapiens	40-42
1992915-2	1991	FdUMP, a metabolite of 5-fluorouracil(5-FU), is known to have an inhibitory activity of thymidylate synthase (TS).	Fluorodeoxyuridylate	0-5	thymidylate synthetase	Homo sapiens	88-108
1557655-1	1992	The inhibition of thymidylate synthase (TS) by the fluorouracil (5-FU) metabolite 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) is considered to be one of the main mechanisms of action of 5-FU.	Fluorodeoxyuridylate	125-130	thymidylate synthetase	Homo sapiens	18-38
1557655-1	1992	The inhibition of thymidylate synthase (TS) by the fluorouracil (5-FU) metabolite 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) is considered to be one of the main mechanisms of action of 5-FU.	Fluorodeoxyuridylate	125-130	thymidylate synthetase	Homo sapiens	40-42
1596583-2	1992	In various tumor cell lines and clinical tumor biopsies, TS mRNA levels correlated with TS levels as determined by [3H]-fluorodeoxyuridylate binding.	Fluorodeoxyuridylate	120-140	thymidylate synthetase	Homo sapiens	57-59
1596583-2	1992	In various tumor cell lines and clinical tumor biopsies, TS mRNA levels correlated with TS levels as determined by [3H]-fluorodeoxyuridylate binding.	Fluorodeoxyuridylate	120-140	thymidylate synthetase	Homo sapiens	88-90
1924359-6	1991	The inclusion of dUMP, 5-fluoro-dUMP, or 5,10-methylene-tetrahydrofolate in in vitro translation reactions completely relieved the inhibition of TS mRNA translation by TS protein.	Fluorodeoxyuridylate	23-36	thymidylate synthetase	Homo sapiens	145-147
1924359-6	1991	The inclusion of dUMP, 5-fluoro-dUMP, or 5,10-methylene-tetrahydrofolate in in vitro translation reactions completely relieved the inhibition of TS mRNA translation by TS protein.	Fluorodeoxyuridylate	23-36	thymidylate synthetase	Homo sapiens	168-170
1992915-2	1991	FdUMP, a metabolite of 5-fluorouracil(5-FU), is known to have an inhibitory activity of thymidylate synthase (TS).	Fluorodeoxyuridylate	0-5	thymidylate synthetase	Homo sapiens	110-112
2244925-6	1990	In the presence of FdUMP or dUMP, all the studied compounds except 7-hydroxymethotrexate exhibited a large negative enthalpy variation when binding to thymidylate synthase (from -44 to -91 kJ/mol).	Fluorodeoxyuridylate	19-24	thymidylate synthetase	Homo sapiens	151-171
1827310-0	1991	Thymidylate synthase from untreated human colorectal cancer and colonic mucosa: enzyme activity and inhibition by 5-fluoro-2"-deoxy-uridine-5"-monophosphate.	Fluorodeoxyuridylate	114-156	thymidylate synthetase	Homo sapiens	0-20
34132895-11	2021	In tumours, there was an association between OAT2 expression and FdUMP concentration.	Fluorodeoxyuridylate	65-70	solute carrier family 22 member 7	Homo sapiens	45-49
1697502-6	1990	Thymidylate synthase (TS) activity was measured by both the 5-fluoro-2"-deoxyuridine-5"-monophosphate binding and catalytic assays, using cytosolic extracts.	Fluorodeoxyuridylate	60-101	thymidylate synthetase	Homo sapiens	0-20
2142214-4	1990	Methotrexate (MTX) pretreatment may also enhance binding of the fluoropyrimidine inhibitor, 5-fluodeoxyuridylate (FdUMP), to the target enzyme, thymidylate synthase (TS), indirectly by increasing dihydrofolate polyglutamates or directly, as MTX polyglutamates, by enhancing the formation of ternary complexes with FdUMP and TS.	Fluorodeoxyuridylate	114-119	thymidylate synthetase	Homo sapiens	144-164
2142214-4	1990	Methotrexate (MTX) pretreatment may also enhance binding of the fluoropyrimidine inhibitor, 5-fluodeoxyuridylate (FdUMP), to the target enzyme, thymidylate synthase (TS), indirectly by increasing dihydrofolate polyglutamates or directly, as MTX polyglutamates, by enhancing the formation of ternary complexes with FdUMP and TS.	Fluorodeoxyuridylate	314-319	thymidylate synthetase	Homo sapiens	144-164
2521810-2	1989	Available evidence suggests that the mechanism of this synergism is a kinetic stabilization of complex formed between thymidylate synthase and fluorodeoxyuridylate that also involves a mole of the cofactor for the thymidylate synthase reaction, 5,10-methylenetetrahydrofolate.	Fluorodeoxyuridylate	143-163	thymidylate synthetase	Homo sapiens	118-138
2729562-2	1989	FAB-MS methods were developed to optimize sensitivity using adenosine 5"-monophosphate as a model compound and then applied to reference standards of two clinically important nucleotides: tricyclic nucleoside-5"-monophosphate (TCNMP) and 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	238-279	FA complementation group B	Homo sapiens	0-3
2729562-2	1989	FAB-MS methods were developed to optimize sensitivity using adenosine 5"-monophosphate as a model compound and then applied to reference standards of two clinically important nucleotides: tricyclic nucleoside-5"-monophosphate (TCNMP) and 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	281-286	FA complementation group B	Homo sapiens	0-3
2521810-2	1989	Available evidence suggests that the mechanism of this synergism is a kinetic stabilization of complex formed between thymidylate synthase and fluorodeoxyuridylate that also involves a mole of the cofactor for the thymidylate synthase reaction, 5,10-methylenetetrahydrofolate.	Fluorodeoxyuridylate	143-163	thymidylate synthetase	Homo sapiens	214-234
2977717-7	1988	Each of these agents modifies the response of tumor cells with the result that the fluorodeoxyuridylate-induced inhibition of thymidylate synthase is maintained.	Fluorodeoxyuridylate	83-103	thymidylate synthetase	Homo sapiens	126-146
3049954-4	1988	However, its conversion to 5-fluoro-2"deoxy-5" monophosphate (FdUMP) leading to inhibition of thymidylate synthase (TS) and subsequently of DNA synthesis, is considered to be its main mechanism of action.	Fluorodeoxyuridylate	62-67	thymidylate synthetase	Homo sapiens	94-114
3126806-1	1987	Thymidylate synthase (TS), 5-fluorodeoxyuridylate (FdUMP), and 5,10-methylenetetrahydrofolate (CH2-H4folate) form a covalent complex in which a Cys thiol of TS is attached to the 6-position of FdUMP and the one-carbon unit of the cofactor is attached to the 5-position.	Fluorodeoxyuridylate	51-56	thymidylate synthetase	Homo sapiens	0-20
2963229-4	1987	The most probable mechanism of the interaction between folinic acid and the fluoropyrimidines is stabilization of thymidylate synthase (TS) in inactive complexes with 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) and folate cofactor.	Fluorodeoxyuridylate	167-208	thymidylate synthetase	Homo sapiens	114-134
3814166-0	1987	Studies on the interaction with thymidylate synthase of analogues of 2"-deoxyuridine-5"-phosphate and 5-fluoro-2"-deoxyuridine-5"-phosphate with modified phosphate groups.	Fluorodeoxyuridylate	102-139	thymidylate synthetase	Homo sapiens	32-52
2963229-4	1987	The most probable mechanism of the interaction between folinic acid and the fluoropyrimidines is stabilization of thymidylate synthase (TS) in inactive complexes with 5-fluoro-2"-deoxyuridine-5"-monophosphate (FdUMP) and folate cofactor.	Fluorodeoxyuridylate	210-215	thymidylate synthetase	Homo sapiens	114-134
3745210-1	1986	The formation of covalent binary complexes of thymidylate synthase and its nucleotide substrate dUMP, product dTMP, and inhibitor, 5-fluorodeoxyuridylate (FdUMP) was investigated using the trichloroacetic acid precipitation method.	Fluorodeoxyuridylate	155-160	thymidylate synthetase	Homo sapiens	46-66
2938731-8	1986	We propose that the elevated TS levels result in sequestration of the reduced-folate pool (as N5,10-methylene tetrahydrofolic acid) into the TS ternary complex with 5-fluoro-2"-deoxyuridine 5"-monophosphate.	Fluorodeoxyuridylate	165-206	thymidylate synthase	Mus musculus	29-31
2938731-8	1986	We propose that the elevated TS levels result in sequestration of the reduced-folate pool (as N5,10-methylene tetrahydrofolic acid) into the TS ternary complex with 5-fluoro-2"-deoxyuridine 5"-monophosphate.	Fluorodeoxyuridylate	165-206	thymidylate synthase	Mus musculus	141-143
31520592-5	2019	Furthermore, the tetrahydrofolate- and time-dependent, covalent binding by thymidylate synthase of each 5-fluoro-dUMP and N4-hydroxy-dCMP was shown to be accompanied by the enzyme inactivation, as well as spectrophotometrically confirmed dihydrofolate production, the latter demonstrated to depend on the reaction time, thymidylate synthase activity and temperature of the incubation mixture, further documenting its catalytic character.	Fluorodeoxyuridylate	104-117	thymidylate synthase	Mus musculus	75-95
3877729-4	1985	In contrast, AE1 cells were highly resistant to almost all other cytotoxic nucleosides including the thymidine analogs, 5-bromodeoxyuridine and 5-fluoro-2"-deoxyuridine 5"-monophosphate.	Fluorodeoxyuridylate	144-185	solute carrier family 4 member 1 (Diego blood group)	Homo sapiens	13-16
3928628-5	1985	Exposure of the double-labeled thymidylate synthetase-FdUMP-[2-14C,7,9,3",5"-3H]5,10-CH2H4folate complex to the Bratton-Marshall reaction resulted in 16% cleavage of the C-9, N-10 bond with release solely of p-aminobenzoylglutamate, whereas all of the carbon-14-labeled pterin residue remained covalently bound to the protein.	Fluorodeoxyuridylate	54-59	complement C9	Homo sapiens	170-173
139127-0	1977	A calorimetric study of the binding of 2"-deoxyuridine-5"-phosphate and 5-fluoro-2"-deoxyuridine-5"-phosphate to thymidylate synthetase.	Fluorodeoxyuridylate	72-109	thymidylate synthetase	Homo sapiens	113-135
31520592-3	2019	Electrophoretic analysis showed mouse recombinant TS protein to form, in the presence of tetrahydrofolate, a covalently bound, electrophoretically separable 5-fluoro-dUMP-thymidylate synthase complex, similar to that produced in the presence of N5,10-methylenetetrahydrofolate.	Fluorodeoxyuridylate	157-170	thymidylate synthase	Mus musculus	171-191
6241433-1	1984	A radiochemical assay for thymidylate synthase (EC 2.1.1.45, dTMP synthase), which permits the accurate determination of total, free, and 5-fluoro-2"-deoxyuridylate (FdUMP)-bound enzyme in cells exposed to the 5-fluoropyrimidine anticancer agents, is described.	Fluorodeoxyuridylate	166-171	thymidylate synthetase	Homo sapiens	26-46
31520592-5	2019	Furthermore, the tetrahydrofolate- and time-dependent, covalent binding by thymidylate synthase of each 5-fluoro-dUMP and N4-hydroxy-dCMP was shown to be accompanied by the enzyme inactivation, as well as spectrophotometrically confirmed dihydrofolate production, the latter demonstrated to depend on the reaction time, thymidylate synthase activity and temperature of the incubation mixture, further documenting its catalytic character.	Fluorodeoxyuridylate	104-117	thymidylate synthase	Mus musculus	320-340
31520592-4	2019	Further studies of the mouse enzyme binding with 5-fluoro-dUMP/N4-hydroxy-dCMP by TCA precipitation of the complex on filter paper showed it to be tetrahydrofolate-promoted, as well as to depend on both time in the range of minutes and the enzyme molecular activity, indicating thymidylate synthase-catalyzed reaction to be responsible for it.	Fluorodeoxyuridylate	49-62	thymidylate synthase	Mus musculus	278-298
30486276-8	2018	MTHF and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP) had combined inhibition and significantly downregulated the expression of thymidylate synthase (TS).	Fluorodeoxyuridylate	9-50	thymidylate synthetase	Homo sapiens	133-153
30486276-8	2018	MTHF and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP) had combined inhibition and significantly downregulated the expression of thymidylate synthase (TS).	Fluorodeoxyuridylate	52-57	thymidylate synthetase	Homo sapiens	133-153
21561529-0	2011	Calpain regulates thymidylate synthase-5-fluoro-dUMP complex levels associated with response to 5-fluorouracil in gastric cancer cells.	Fluorodeoxyuridylate	39-52	thymidylate synthetase	Homo sapiens	18-38
21561529-1	2011	Thymidylate synthase (TS) plays a major role in the response to 5-fluorouracil (5-FU) by binding directly to the 5-FU metabolite, 5-fluoro-dUMP (FdUMP).	Fluorodeoxyuridylate	130-143	thymidylate synthetase	Homo sapiens	0-20
21561529-1	2011	Thymidylate synthase (TS) plays a major role in the response to 5-fluorouracil (5-FU) by binding directly to the 5-FU metabolite, 5-fluoro-dUMP (FdUMP).	Fluorodeoxyuridylate	145-150	thymidylate synthetase	Homo sapiens	0-20
17237272-1	2007	Multidrug-resistance-associated protein, MRP8/ABCC11 (ABCC11), is an efflux pump for nucleotide analogues and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	110-151	ATP binding cassette subfamily C member 3	Homo sapiens	0-39
17237272-1	2007	Multidrug-resistance-associated protein, MRP8/ABCC11 (ABCC11), is an efflux pump for nucleotide analogues and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	110-151	ATP binding cassette subfamily C member 11	Homo sapiens	41-45
17237272-1	2007	Multidrug-resistance-associated protein, MRP8/ABCC11 (ABCC11), is an efflux pump for nucleotide analogues and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	110-151	ATP binding cassette subfamily C member 11	Homo sapiens	46-52
17237272-1	2007	Multidrug-resistance-associated protein, MRP8/ABCC11 (ABCC11), is an efflux pump for nucleotide analogues and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	110-151	ATP binding cassette subfamily C member 11	Homo sapiens	54-60
17237272-1	2007	Multidrug-resistance-associated protein, MRP8/ABCC11 (ABCC11), is an efflux pump for nucleotide analogues and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	153-158	ATP binding cassette subfamily C member 3	Homo sapiens	0-39
17237272-1	2007	Multidrug-resistance-associated protein, MRP8/ABCC11 (ABCC11), is an efflux pump for nucleotide analogues and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	153-158	ATP binding cassette subfamily C member 11	Homo sapiens	41-45
17237272-1	2007	Multidrug-resistance-associated protein, MRP8/ABCC11 (ABCC11), is an efflux pump for nucleotide analogues and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	153-158	ATP binding cassette subfamily C member 11	Homo sapiens	46-52
17237272-1	2007	Multidrug-resistance-associated protein, MRP8/ABCC11 (ABCC11), is an efflux pump for nucleotide analogues and 5-fluoro-2"-deoxyuridine 5"-monophosphate (FdUMP).	Fluorodeoxyuridylate	153-158	ATP binding cassette subfamily C member 11	Homo sapiens	54-60
26916840-0	2016	Phosphorylation of thymidylate synthase affects slow-binding inhibition by 5-fluoro-dUMP and N(4)-hydroxy-dCMP.	Fluorodeoxyuridylate	75-88	thymidylate synthase	Mus musculus	19-39
23641929-0	2013	Localization of putative binding sites for cyclic guanosine monophosphate and the anti-cancer drug 5-fluoro-2"-deoxyuridine-5"-monophosphate on ABCC11 in silico models.	Fluorodeoxyuridylate	99-140	ATP binding cassette subfamily C member 11	Homo sapiens	144-150