PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
8028591-3	1994	Serine esterase inhibitors phenylmethylsulfonylfluoride and diisopropyl fluorophosphate prevent the increase in serine esterase and phospholipase A2 activities and arachidonic acid release caused by A23187.	Phenylmethylsulfonyl Fluoride	27-55	phospholipase A2	Oryctolagus cuniculus	132-148
7511097-5	1994	The IGFBP-5 protease activity was inhibited by EDTA, phenanthroline and PMSF.	Phenylmethylsulfonyl Fluoride	72-76	HtrA serine peptidase 1	Homo sapiens	4-20
8187221-7	1994	Treatment of R-apo(a) with phenylmethanesulfonyl fluoride inhibited LDL apoB-100 degradation, indicating that R-apo(a) has serine esterase type proteolytic activity.	Phenylmethylsulfonyl Fluoride	27-57	apolipoprotein B	Homo sapiens	72-80
8068240-5	1994	Loss of UCP immunoreactivity was markedly reduced when membranes were incubated at 4 degrees C or in the presence of phenylmethylsulfonyl fluoride, but was not influenced by the addition of GDP.	Phenylmethylsulfonyl Fluoride	117-146	uncoupling protein 1	Rattus norvegicus	8-11
16349084-9	1993	Phenylmethylsulfonyl fluoride and EDTA, inhibitors of serine and metalloproteases, strongly or completely inhibited WH 7803 aminopeptidase.	Phenylmethylsulfonyl Fluoride	0-29	carboxypeptidase Q	Homo sapiens	124-138
7947482-5	1994	Although some radioactivity was released by buffer controls, upon the addition of CE, a burst of radiolabel release occurred which was due to an enzymatic reaction that could be saturated and inhibited by the specific esterase inhibitor, phenylmethylsulfonylfluoride.	Phenylmethylsulfonyl Fluoride	238-266	carboxyl ester lipase	Homo sapiens	82-84
8463250-10	1993	Neutrophil elastase inactivated with phenylmethylsulfonyl fluoride did not compete with active elastase for binding to thrombospondin, implying that a functional active site is important for the interaction of elastase with thrombospondin.	Phenylmethylsulfonyl Fluoride	37-66	elastase, neutrophil expressed	Homo sapiens	0-19
8228252-5	1993	PMSF (2 mM) also blocked receptors shedding from paraformaldehyde-fixed THP-1 cells coincubated with conditioned media from PMA-stimulated THP-1 cells.	Phenylmethylsulfonyl Fluoride	0-4	GLI family zinc finger 2	Homo sapiens	72-77
8228252-5	1993	PMSF (2 mM) also blocked receptors shedding from paraformaldehyde-fixed THP-1 cells coincubated with conditioned media from PMA-stimulated THP-1 cells.	Phenylmethylsulfonyl Fluoride	0-4	GLI family zinc finger 2	Homo sapiens	139-144
8298579-0	1993	Evaluation of phenylmethanesulfonyl fluoride (PMSF) as a tracer candidate mapping acetylcholinesterase in vivo.	Phenylmethylsulfonyl Fluoride	14-44	acetylcholinesterase	Rattus norvegicus	82-102
8298579-0	1993	Evaluation of phenylmethanesulfonyl fluoride (PMSF) as a tracer candidate mapping acetylcholinesterase in vivo.	Phenylmethylsulfonyl Fluoride	46-50	acetylcholinesterase	Rattus norvegicus	82-102
8298579-1	1993	The availability of phenylmethanesulfonyl fluoride (PMSF), an irreversible cholinesterase inhibitor, for a tracer mapping acetylcholinesterase (AchE) in vivo in brain and other organs was evaluated using [35S]PMSF in mice and rats.	Phenylmethylsulfonyl Fluoride	20-50	butyrylcholinesterase	Mus musculus	75-89
8298579-1	1993	The availability of phenylmethanesulfonyl fluoride (PMSF), an irreversible cholinesterase inhibitor, for a tracer mapping acetylcholinesterase (AchE) in vivo in brain and other organs was evaluated using [35S]PMSF in mice and rats.	Phenylmethylsulfonyl Fluoride	20-50	acetylcholinesterase	Rattus norvegicus	122-142
8298579-1	1993	The availability of phenylmethanesulfonyl fluoride (PMSF), an irreversible cholinesterase inhibitor, for a tracer mapping acetylcholinesterase (AchE) in vivo in brain and other organs was evaluated using [35S]PMSF in mice and rats.	Phenylmethylsulfonyl Fluoride	20-50	acetylcholinesterase	Mus musculus	144-148
8298579-1	1993	The availability of phenylmethanesulfonyl fluoride (PMSF), an irreversible cholinesterase inhibitor, for a tracer mapping acetylcholinesterase (AchE) in vivo in brain and other organs was evaluated using [35S]PMSF in mice and rats.	Phenylmethylsulfonyl Fluoride	52-56	butyrylcholinesterase	Mus musculus	75-89
8298579-1	1993	The availability of phenylmethanesulfonyl fluoride (PMSF), an irreversible cholinesterase inhibitor, for a tracer mapping acetylcholinesterase (AchE) in vivo in brain and other organs was evaluated using [35S]PMSF in mice and rats.	Phenylmethylsulfonyl Fluoride	52-56	acetylcholinesterase	Rattus norvegicus	122-142
8298579-1	1993	The availability of phenylmethanesulfonyl fluoride (PMSF), an irreversible cholinesterase inhibitor, for a tracer mapping acetylcholinesterase (AchE) in vivo in brain and other organs was evaluated using [35S]PMSF in mice and rats.	Phenylmethylsulfonyl Fluoride	52-56	acetylcholinesterase	Mus musculus	144-148
8374612-5	1993	LpL is inhibited by the serine protease inhibitors diisopropylfluorophosphate (DFP) and phenylmethanesulfonyl fluoride (PMSF), which indicates the presence of an active site serine similar to mammalian LpL.	Phenylmethylsulfonyl Fluoride	88-118	lipoprotein lipase	Homo sapiens	0-3
8374612-5	1993	LpL is inhibited by the serine protease inhibitors diisopropylfluorophosphate (DFP) and phenylmethanesulfonyl fluoride (PMSF), which indicates the presence of an active site serine similar to mammalian LpL.	Phenylmethylsulfonyl Fluoride	120-124	lipoprotein lipase	Homo sapiens	0-3
8374612-5	1993	LpL is inhibited by the serine protease inhibitors diisopropylfluorophosphate (DFP) and phenylmethanesulfonyl fluoride (PMSF), which indicates the presence of an active site serine similar to mammalian LpL.	Phenylmethylsulfonyl Fluoride	120-124	lipoprotein lipase	Homo sapiens	202-205
8376994-10	1993	This enzyme, which removes the C-terminal region of CPE, is partially inhibited by EDTA and phenylmethylsulfonyl fluoride and is activated by CaCl2.	Phenylmethylsulfonyl Fluoride	92-121	carboxypeptidase E	Mus musculus	52-55
8211998-12	1993	Different levels of NTE inhibition as caused by different compounds were promoted by the same dose of phenylmethanesulfonyl fluoride to similar degrees of ataxia.	Phenylmethylsulfonyl Fluoride	102-132	patatin like phospholipase domain containing 6	Homo sapiens	20-23
7763983-6	1993	The soybean protease was significantly inhibited by PMSF, indicating the endopeptidase is a serine protease.	Phenylmethylsulfonyl Fluoride	52-56	subtilisin-like protease C1	Glycine max	92-107
8407286-7	1993	Rat peritoneal cells showed a significant ability to catabolize PAF by intracellular PAF-acetylhydrolase in view of both the amounts of enzyme activity assayed in cellular homogenates, and the 15-fold increase on controls of PAF quantities detected in peritoneal cells treated with phenylmethylsulfonyl fluoride (PMSF), a known inhibitor of PAF-acetylhydrolase.	Phenylmethylsulfonyl Fluoride	282-311	PCNA clamp associated factor	Rattus norvegicus	64-67
8407286-7	1993	Rat peritoneal cells showed a significant ability to catabolize PAF by intracellular PAF-acetylhydrolase in view of both the amounts of enzyme activity assayed in cellular homogenates, and the 15-fold increase on controls of PAF quantities detected in peritoneal cells treated with phenylmethylsulfonyl fluoride (PMSF), a known inhibitor of PAF-acetylhydrolase.	Phenylmethylsulfonyl Fluoride	282-311	PCNA clamp associated factor	Rattus norvegicus	85-88
8407286-7	1993	Rat peritoneal cells showed a significant ability to catabolize PAF by intracellular PAF-acetylhydrolase in view of both the amounts of enzyme activity assayed in cellular homogenates, and the 15-fold increase on controls of PAF quantities detected in peritoneal cells treated with phenylmethylsulfonyl fluoride (PMSF), a known inhibitor of PAF-acetylhydrolase.	Phenylmethylsulfonyl Fluoride	282-311	PCNA clamp associated factor	Rattus norvegicus	85-88
8407286-7	1993	Rat peritoneal cells showed a significant ability to catabolize PAF by intracellular PAF-acetylhydrolase in view of both the amounts of enzyme activity assayed in cellular homogenates, and the 15-fold increase on controls of PAF quantities detected in peritoneal cells treated with phenylmethylsulfonyl fluoride (PMSF), a known inhibitor of PAF-acetylhydrolase.	Phenylmethylsulfonyl Fluoride	282-311	PCNA clamp associated factor	Rattus norvegicus	85-88
8407286-7	1993	Rat peritoneal cells showed a significant ability to catabolize PAF by intracellular PAF-acetylhydrolase in view of both the amounts of enzyme activity assayed in cellular homogenates, and the 15-fold increase on controls of PAF quantities detected in peritoneal cells treated with phenylmethylsulfonyl fluoride (PMSF), a known inhibitor of PAF-acetylhydrolase.	Phenylmethylsulfonyl Fluoride	313-317	PCNA clamp associated factor	Rattus norvegicus	64-67
8407286-7	1993	Rat peritoneal cells showed a significant ability to catabolize PAF by intracellular PAF-acetylhydrolase in view of both the amounts of enzyme activity assayed in cellular homogenates, and the 15-fold increase on controls of PAF quantities detected in peritoneal cells treated with phenylmethylsulfonyl fluoride (PMSF), a known inhibitor of PAF-acetylhydrolase.	Phenylmethylsulfonyl Fluoride	313-317	PCNA clamp associated factor	Rattus norvegicus	85-88
8407286-7	1993	Rat peritoneal cells showed a significant ability to catabolize PAF by intracellular PAF-acetylhydrolase in view of both the amounts of enzyme activity assayed in cellular homogenates, and the 15-fold increase on controls of PAF quantities detected in peritoneal cells treated with phenylmethylsulfonyl fluoride (PMSF), a known inhibitor of PAF-acetylhydrolase.	Phenylmethylsulfonyl Fluoride	313-317	PCNA clamp associated factor	Rattus norvegicus	85-88
8407286-7	1993	Rat peritoneal cells showed a significant ability to catabolize PAF by intracellular PAF-acetylhydrolase in view of both the amounts of enzyme activity assayed in cellular homogenates, and the 15-fold increase on controls of PAF quantities detected in peritoneal cells treated with phenylmethylsulfonyl fluoride (PMSF), a known inhibitor of PAF-acetylhydrolase.	Phenylmethylsulfonyl Fluoride	313-317	PCNA clamp associated factor	Rattus norvegicus	85-88
8333546-4	1993	Serine esterase inhibitors, such as phenylmethylsulfonyl fluoride and diisopropyl fluorophosphate, prevent the A23187-mediated increase in serine esterase activity, PLA2 activity, and AA release.	Phenylmethylsulfonyl Fluoride	36-65	LOC104974671	Bos taurus	165-169
8343997-3	1993	Brain neuropathy target esterase (NTE) has been preinhibited with phenylmethylsulphonyl fluoride (PMSF) (0, 5, 10, 15, 30 and 60 microM) or with diisopropylphoshoro fluoridate (DFP) (0, 0.2, 0.5, and 1 microM) at 37 degrees C for 30 min.	Phenylmethylsulfonyl Fluoride	66-96	patatin like phospholipase domain containing 6	Homo sapiens	6-32
8343997-3	1993	Brain neuropathy target esterase (NTE) has been preinhibited with phenylmethylsulphonyl fluoride (PMSF) (0, 5, 10, 15, 30 and 60 microM) or with diisopropylphoshoro fluoridate (DFP) (0, 0.2, 0.5, and 1 microM) at 37 degrees C for 30 min.	Phenylmethylsulfonyl Fluoride	66-96	patatin like phospholipase domain containing 6	Homo sapiens	34-37
8343997-3	1993	Brain neuropathy target esterase (NTE) has been preinhibited with phenylmethylsulphonyl fluoride (PMSF) (0, 5, 10, 15, 30 and 60 microM) or with diisopropylphoshoro fluoridate (DFP) (0, 0.2, 0.5, and 1 microM) at 37 degrees C for 30 min.	Phenylmethylsulfonyl Fluoride	98-102	patatin like phospholipase domain containing 6	Homo sapiens	6-32
8343997-3	1993	Brain neuropathy target esterase (NTE) has been preinhibited with phenylmethylsulphonyl fluoride (PMSF) (0, 5, 10, 15, 30 and 60 microM) or with diisopropylphoshoro fluoridate (DFP) (0, 0.2, 0.5, and 1 microM) at 37 degrees C for 30 min.	Phenylmethylsulfonyl Fluoride	98-102	patatin like phospholipase domain containing 6	Homo sapiens	34-37
8344002-6	1993	These prophylactic and promoting effects are similar to those exerted by phenylmethanesulphonyl fluoride (PMSF) at doses which inhibit NTE.	Phenylmethylsulfonyl Fluoride	73-104	patatin like phospholipase domain containing 6	Gallus gallus	135-138
8344002-6	1993	These prophylactic and promoting effects are similar to those exerted by phenylmethanesulphonyl fluoride (PMSF) at doses which inhibit NTE.	Phenylmethylsulfonyl Fluoride	106-110	patatin like phospholipase domain containing 6	Gallus gallus	135-138
8495756-3	1993	The proteolytic activity is inhibited by heat-denaturation and known proteinase inhibitors (iodoacetate and phenylmethylsulfonyl fluoride), and the pattern of deteriosome-associated proteinase activity changes with advancing senescence of the cotyledon tissue.	Phenylmethylsulfonyl Fluoride	108-137	endogenous retrovirus group K member 25	Homo sapiens	69-79
8495756-3	1993	The proteolytic activity is inhibited by heat-denaturation and known proteinase inhibitors (iodoacetate and phenylmethylsulfonyl fluoride), and the pattern of deteriosome-associated proteinase activity changes with advancing senescence of the cotyledon tissue.	Phenylmethylsulfonyl Fluoride	108-137	endogenous retrovirus group K member 25	Homo sapiens	182-192
8407286-8	1993	The PAF activity produced upon PMSF addition showed a retention time on reverse-phase HPLC which suggests structural identity to PAF produced by either immunological challenge or ionophore A23187.	Phenylmethylsulfonyl Fluoride	31-35	PCNA clamp associated factor	Rattus norvegicus	4-7
8407286-8	1993	The PAF activity produced upon PMSF addition showed a retention time on reverse-phase HPLC which suggests structural identity to PAF produced by either immunological challenge or ionophore A23187.	Phenylmethylsulfonyl Fluoride	31-35	PCNA clamp associated factor	Rattus norvegicus	129-132
8343996-5	1993	Additionally, whereas developing chickens are typically resistant to the effects of neuropathic OPs, resistant age groups will develop OPIDN when exposure to a neuropathic OP is followed by the non-neuropathic NTE inhibitor phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	224-253	patatin like phospholipase domain containing 6	Gallus gallus	210-213
8342171-5	1993	The factor II- and X-activator components could be inhibited by EDTA, EGTA and 2-mercaptoethanol, whereas the thrombin-like activity was inhibited by PMSF.	Phenylmethylsulfonyl Fluoride	150-154	coagulation factor II, thrombin	Homo sapiens	110-118
8458433-3	1993	The lipoprotein ECE activity, which was optimal at pH 7.0, was inhibited by EDTA, o-phenanthroline, phosphoramidon, thiorphan, phenylmethanesulfonyl fluoride and chymostatin, but not by cysteine or aspartic proteinase inhibitors, suggesting metalloproteinase- and chymotrypsin-like properties.	Phenylmethylsulfonyl Fluoride	127-157	endothelin converting enzyme 1	Homo sapiens	16-19
8463250-10	1993	Neutrophil elastase inactivated with phenylmethylsulfonyl fluoride did not compete with active elastase for binding to thrombospondin, implying that a functional active site is important for the interaction of elastase with thrombospondin.	Phenylmethylsulfonyl Fluoride	37-66	elastase, neutrophil expressed	Homo sapiens	11-19
8436976-9	1993	Inactivation of alpha-thrombin with D-phenyl-alanyl-L-propyl-L-arginine chloromethyl ketone, phenylmethylsulfonyl fluoride, antithrombin III, or hirudin results in a marked decrease of the stimulatory effect.	Phenylmethylsulfonyl Fluoride	93-122	coagulation factor II	Rattus norvegicus	22-30
8514423-5	1993	In SP, PAF-AH-like activity was Ca(++)-independent, acid and heat labile, stable to freezing, not inhibited by phosphatidylcholine, but was inhibited by 10 mM disopropylfluorophosphate (DFP) and 13 mM phenylmethylsulfonylfluoride (PMSF).	Phenylmethylsulfonyl Fluoride	201-229	phospholipase A2 group VII	Homo sapiens	7-13
8514423-5	1993	In SP, PAF-AH-like activity was Ca(++)-independent, acid and heat labile, stable to freezing, not inhibited by phosphatidylcholine, but was inhibited by 10 mM disopropylfluorophosphate (DFP) and 13 mM phenylmethylsulfonylfluoride (PMSF).	Phenylmethylsulfonyl Fluoride	231-235	phospholipase A2 group VII	Homo sapiens	7-13
8483812-8	1993	The beneficial effects of PMSF and EGTA suggested that serine protease(s) and metalloproteases contribute to the observed anomalous pharmacological characteristics of AI and AIII, respectively.	Phenylmethylsulfonyl Fluoride	26-30	cell division cycle 34, ubiqiutin conjugating enzyme	Rattus norvegicus	55-70
7678848-5	1993	When protease inhibitors, such as aprotinin or phenylmethanesulfonyl fluoride, were present during quartz exposure, the down-regulation of CR1 was less pronounced, but this was not the case not when protease inhibitors such as EDTA-Na2 and pepstatin were present.	Phenylmethylsulfonyl Fluoride	47-77	complement C3b/C4b receptor 1 (Knops blood group)	Homo sapiens	139-142
8417829-9	1993	The enzymatic activity of cathepsin-G was found to be required for the lysis of these tumor cells, since phenylmethylsulfonyl fluoride blocks the lytic ability of this protein.	Phenylmethylsulfonyl Fluoride	105-134	cathepsin G	Homo sapiens	26-37
8381002-11	1993	In 36-day-old chicks, PMSF (300 mg/kg) promoted OPIDP when given up to 5 days after DFP (1.5 mg/kg) when residual NTE inhibition in brain and sciatic nerve was about 40%.	Phenylmethylsulfonyl Fluoride	22-26	patatin like phospholipase domain containing 6	Gallus gallus	114-117
1288504-3	1992	The enzyme exhibited serine-protease properties by showing susceptibility to phenyl methyl sulfonyl fluoride, alpha 1-antitrypsin, and egg white and soybean inhibitors.	Phenylmethylsulfonyl Fluoride	77-108	subtilisin-like protease C1	Glycine max	21-36
1450218-10	1992	The lysophospholipase activities of peaks 1 and 2, but not peak 3, were inhibited by phenylmethylsulfonyl fluoride, diisopropyl fluorophosphate and N-ethylmaleimide.	Phenylmethylsulfonyl Fluoride	85-114	pseudopodium-enriched atypical kinase 1	Mus musculus	36-49
1358626-8	1992	Indeed, the decrease in CD43 expression was inhibited by phenylmethanesulfonylfluoride (PMSF).	Phenylmethylsulfonyl Fluoride	88-92	sialophorin	Homo sapiens	24-28
1330373-4	1992	At pH 7.4, phenylmethylsulfonyl fluoride increased the potencies of the three renin inhibitors slightly (< or = 43%), whereas IC50 values determined in the presence of 8-hydroxyquinoline decreased by 1.5- to 3.7-fold.	Phenylmethylsulfonyl Fluoride	11-40	renin	Homo sapiens	78-83
1330373-6	1992	The results show that any dissociation of the hypotensive activity measured in vivo from the plasma renin activity measured in vitro is not simply an artifact in the plasma renin activity assay stemming from the use of these angiotensinase inhibitors, especially if only phenylmethylsulfonyl fluoride is used.	Phenylmethylsulfonyl Fluoride	271-300	renin	Homo sapiens	100-105
1412529-3	1992	Pre-treatment with non-neuropathic NTE inhibitors, such as phenylmethanesulfonyl fluoride (PMSF), protects from OPIDP.	Phenylmethylsulfonyl Fluoride	59-89	patatin-like phospholipase domain containing 6	Rattus norvegicus	35-38
16653094-7	1992	Degradation of this enzyme protein was moderately inhibited by the administration of aminooxyacetic acid, a competitive inhibitor of ACC synthase with respect to its substrate S-adenosyl-l-methionine, alpha,alpha"-dipyridyl, and phenylmethanesulfonyl fluoride or leupeptin, serine protease inhibitors.	Phenylmethylsulfonyl Fluoride	229-259	serine protease	Solanum lycopersicum	274-289
1400575-18	1992	In the presence of PMSF, wild-type cells accumulated autophagic bodies in the vacuoles under nutrient-deficient conditions in the same manner as did multiple protease-deficient mutants or cells with a disrupted PRB1 gene.	Phenylmethylsulfonyl Fluoride	19-23	proteinase B	Saccharomyces cerevisiae S288C	211-215
1412529-3	1992	Pre-treatment with non-neuropathic NTE inhibitors, such as phenylmethanesulfonyl fluoride (PMSF), protects from OPIDP.	Phenylmethylsulfonyl Fluoride	91-95	patatin-like phospholipase domain containing 6	Rattus norvegicus	35-38
1379257-9	1992	IGFBP-3 proteolytic activity in postoperative sera was markedly inhibited by antipain, Na-p-tosyl-L-lysine chloromethyl ketone, phenylmethylsulfonylfluoride, aprotinin, o-phenanthroline, and EDTA, but not by leupeptin or N-tosyl-L-phenylalanine chloromethyl ketone.	Phenylmethylsulfonyl Fluoride	128-156	insulin like growth factor binding protein 3	Homo sapiens	0-7
1530582-8	1992	PMSF-treated BMMC produced larger quantities of AAGPC than of PAF.	Phenylmethylsulfonyl Fluoride	0-4	patchy fur	Mus musculus	62-65
1530582-9	1992	The AAGPC/PAF ratio detected in PMSF-treated BMMC was very similar to the ratio of arachidonate contained in and released from 1-acyl-/1-alkyl-linked phosphatidylcholine (PC).	Phenylmethylsulfonyl Fluoride	32-36	patchy fur	Mus musculus	10-13
1368252-5	1992	The activity was significantly inhibited by phenylmethylsulfonyl fluoride, indicating the endopeptidase is a serine protease.	Phenylmethylsulfonyl Fluoride	44-73	subtilisin-like protease C1	Glycine max	109-124
1495979-3	1992	The processing of bET to all its metabolites including ET-1 was prevented by the serine protease inhibitor 3,4-dichloroisocoumarin (DCI; 50 microM) or the elastase inhibitor ONO-5046 (100 microM) but not by phenylmethylsulfonyl fluoride (PMSF; 143 microM), another serine protease inhibitor.	Phenylmethylsulfonyl Fluoride	207-236	endothelin 1	Homo sapiens	55-59
1495979-3	1992	The processing of bET to all its metabolites including ET-1 was prevented by the serine protease inhibitor 3,4-dichloroisocoumarin (DCI; 50 microM) or the elastase inhibitor ONO-5046 (100 microM) but not by phenylmethylsulfonyl fluoride (PMSF; 143 microM), another serine protease inhibitor.	Phenylmethylsulfonyl Fluoride	238-242	endothelin 1	Homo sapiens	55-59
1495979-8	1992	The degradation of ET-1 by PMN microsomes was prevented by DCI, PMSF, or ONO-5046.	Phenylmethylsulfonyl Fluoride	64-68	endothelin 1	Homo sapiens	19-23
1873479-6	1991	In both situations, the generation of NAP-2 could be prevented by serine-protease inhibitor phenylmethylsulfonyl fluoride but not by inhibitors specific for Ca(2+)-dependent or thiol proteases.	Phenylmethylsulfonyl Fluoride	92-121	pro-platelet basic protein	Homo sapiens	38-43
1603092-3	1992	Paradoxically, it has been found that the general serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF) frequently cannot block killing even though it inhibits many of the serine proteases.	Phenylmethylsulfonyl Fluoride	107-111	coagulation factor II, thrombin	Homo sapiens	50-65
1584798-6	1992	One of these activities was a PEP4-dependent carboxypeptidase that was sensitive to phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	84-113	proteinase A	Saccharomyces cerevisiae S288C	30-34
1572909-2	1992	One activity removing dipeptides from the NH2-terminal end of Gly-Pro-pNA was specifically inhibited by di-isopropyl-fluorophosphate (DFP), phenylmethanesulphony fluoride (PMSF), and diprotin A, and thus was identified as dipeptidyl peptidase IV (DPP IV).	Phenylmethylsulfonyl Fluoride	172-176	dipeptidyl peptidase 4	Homo sapiens	222-245
1572909-2	1992	One activity removing dipeptides from the NH2-terminal end of Gly-Pro-pNA was specifically inhibited by di-isopropyl-fluorophosphate (DFP), phenylmethanesulphony fluoride (PMSF), and diprotin A, and thus was identified as dipeptidyl peptidase IV (DPP IV).	Phenylmethylsulfonyl Fluoride	172-176	dipeptidyl peptidase 4	Homo sapiens	247-253
1655937-7	1991	The release of radiolabelled fibronectin by stimulated PMNL was blocked in a dose-dependent manner by the addition of both methoxysuccinyl-alanine-alanine-valine chloromethyl ketone (AAPVCK), a leukocyte elastase specific inhibitor as well as phenylmethylsulfonylfluoride (PMSF), a non-specific serine protease inhibitor.	Phenylmethylsulfonyl Fluoride	243-271	fibronectin 1	Rattus norvegicus	29-40
1655937-7	1991	The release of radiolabelled fibronectin by stimulated PMNL was blocked in a dose-dependent manner by the addition of both methoxysuccinyl-alanine-alanine-valine chloromethyl ketone (AAPVCK), a leukocyte elastase specific inhibitor as well as phenylmethylsulfonylfluoride (PMSF), a non-specific serine protease inhibitor.	Phenylmethylsulfonyl Fluoride	273-277	fibronectin 1	Rattus norvegicus	29-40
1908796-2	1991	This activity is mainly distributed in the microsomal fraction (76.5% of total) and has properties similar to the mammalian PAF-acetylhydrolase since it is Ca(2+)-independent, acid-labile, is inhibited by DFP and PMSF but it is not affected by egg yolk phosphatidylcholine.	Phenylmethylsulfonyl Fluoride	213-217	phospholipase A2 group VII	Homo sapiens	124-143
1898045-2	1991	Retinol bound to cellular retinol-binding protein (CRBP) or dispersed in solvent was esterified in a fatty acyl CoA-independent, PMSF-sensitive reaction, consistent with lecithin:retinol acyltransferase (LRAT) activity.	Phenylmethylsulfonyl Fluoride	129-133	retinol binding protein 1	Rattus norvegicus	17-49
1898045-2	1991	Retinol bound to cellular retinol-binding protein (CRBP) or dispersed in solvent was esterified in a fatty acyl CoA-independent, PMSF-sensitive reaction, consistent with lecithin:retinol acyltransferase (LRAT) activity.	Phenylmethylsulfonyl Fluoride	129-133	retinol binding protein 1	Rattus norvegicus	51-55
1930284-7	1991	Analysis of inhibition kinetics further showed that (a) the reversible inhibition of both ComEst and MonEst by sodium fluoride (NaF) was noncompetitive (with Ki values of 1.28 and 0.01 mM, respectively, indicating a marked difference in sensitivity); (b) the inhibition of MonEst by PMSF was of "mixed" noncompetitive-competitive type; and (c) that DEPC exerted noncompetitive inhibition with similar Ki values (0.05 mM) for both esterase species.	Phenylmethylsulfonyl Fluoride	283-287	C-X-C motif chemokine ligand 8	Homo sapiens	128-131
1937496-4	1991	P57 proteinase eluted from MP1-sepharose was inhibited by 5 x 10(-4) M PMSF, enhanced by 0.5% SDS and generated C3 fragments identical to those generated by membrane crude extract of human erythrocytes.	Phenylmethylsulfonyl Fluoride	71-75	cyclin dependent kinase inhibitor 1C	Homo sapiens	0-3
1937496-4	1991	P57 proteinase eluted from MP1-sepharose was inhibited by 5 x 10(-4) M PMSF, enhanced by 0.5% SDS and generated C3 fragments identical to those generated by membrane crude extract of human erythrocytes.	Phenylmethylsulfonyl Fluoride	71-75	late endosomal/lysosomal adaptor, MAPK and MTOR activator 3	Homo sapiens	27-30
1940628-10	1991	The bacterial fusion protein, the CHO-produced lipase, and native rat hepatic lipase were all inhibited by phenylmethylsulfonyl fluoride, implying that they function catalytically as serine esterases.	Phenylmethylsulfonyl Fluoride	107-136	lipase G, endothelial type	Rattus norvegicus	47-53
1940628-10	1991	The bacterial fusion protein, the CHO-produced lipase, and native rat hepatic lipase were all inhibited by phenylmethylsulfonyl fluoride, implying that they function catalytically as serine esterases.	Phenylmethylsulfonyl Fluoride	107-136	lipase C, hepatic type	Rattus norvegicus	70-84
2039753-2	1991	Single point saturation analysis and Scatchard analysis demonstrated that both 5 mM PMSF and 5 mM DFP were able to inhibit steroid binding to the ER after incubation at 37 degrees C, but neither were able to inhibit steroid binding of the nonactivated ER (0-4 degrees C).	Phenylmethylsulfonyl Fluoride	84-88	estrogen receptor 1	Rattus norvegicus	146-148
2039753-2	1991	Single point saturation analysis and Scatchard analysis demonstrated that both 5 mM PMSF and 5 mM DFP were able to inhibit steroid binding to the ER after incubation at 37 degrees C, but neither were able to inhibit steroid binding of the nonactivated ER (0-4 degrees C).	Phenylmethylsulfonyl Fluoride	84-88	estrogen receptor 1	Rattus norvegicus	252-254
1826080-11	1991	Inhibition experiments with phenylmethanesulphonyl fluoride, carbodi-imide, dichloroisocoumarin and competitive peptide inhibitors demonstrated that Lp(a) has enzyme activity that closely resembles that of serine proteinases.	Phenylmethylsulfonyl Fluoride	28-59	lipoprotein(a)	Homo sapiens	149-154
2017753-4	1991	PMSF and other protective chemicals inhibit NTE but OPIDP does not develop because aging cannot occur.	Phenylmethylsulfonyl Fluoride	0-4	patatin like phospholipase domain containing 6	Gallus gallus	44-47
2017753-9	1991	PMSF increased NTE inhibition to greater than 90%.	Phenylmethylsulfonyl Fluoride	0-4	patatin like phospholipase domain containing 6	Gallus gallus	15-18
2017753-13	1991	Either effect is always related to the doses of PMSF, which inhibit NTE.	Phenylmethylsulfonyl Fluoride	48-52	patatin like phospholipase domain containing 6	Gallus gallus	68-71
1964457-6	1990	The esterase activity was strongly inhibited by diisopropylfluorophosphate and phenylmethanesulfonyl fluoride, and to a lesser extent by tosyl-L-lysine chloromethyl ketone, indicating that the enzyme is a serine protease like thrombin.	Phenylmethylsulfonyl Fluoride	79-109	coagulation factor II, thrombin	Bos taurus	226-234
1993057-4	1991	Incubations of activated PMNs with PMSF significantly potentiated the generation of ET-1 like activity and selectively inhibited the degradation of [125I]ET-1 by activated PMNs.	Phenylmethylsulfonyl Fluoride	35-39	endothelin 1	Homo sapiens	84-88
1993057-4	1991	Incubations of activated PMNs with PMSF significantly potentiated the generation of ET-1 like activity and selectively inhibited the degradation of [125I]ET-1 by activated PMNs.	Phenylmethylsulfonyl Fluoride	35-39	endothelin 1	Homo sapiens	154-158
1900206-8	1991	The activity of this enzyme was decreased by the serine esterase inhibitors phenylmethyl-sulfonyl fluoride and N-tosyl-L-phenylalanine chloromethyl ketone and by the histidine modifier diethyl pyrocarbonate, suggesting that CoA-IT may belong to a family of acyltransferase enzymes typified by LCAT.	Phenylmethylsulfonyl Fluoride	76-106	lecithin-cholesterol acyltransferase	Homo sapiens	293-297
1725374-2	1991	The formation of this ET-1-like activity from bET was partially inhibited by phosphoramidon (54 micrograms/ml), but not by pepstatin-A (1 microgram/ml), epoxysuccinyl-L-leucylamido(guanidino)butane (E-64, 10 micrograms/ml) or phenylmethylsulfonyl fluoride (PMSF, 25 micrograms/ml).	Phenylmethylsulfonyl Fluoride	226-255	endothelin 1	Homo sapiens	22-26
1725374-2	1991	The formation of this ET-1-like activity from bET was partially inhibited by phosphoramidon (54 micrograms/ml), but not by pepstatin-A (1 microgram/ml), epoxysuccinyl-L-leucylamido(guanidino)butane (E-64, 10 micrograms/ml) or phenylmethylsulfonyl fluoride (PMSF, 25 micrograms/ml).	Phenylmethylsulfonyl Fluoride	257-261	endothelin 1	Homo sapiens	22-26
1725374-5	1991	The metabolism of [125I]ET-1 by PMNs or leukocyte cathepsin G (5 micrograms/ml) was prevented by PMSF (25 micrograms/ml), but not by phosphoramidon (54 micrograms/ml) or pepstatin-A (1 microgram/ml).	Phenylmethylsulfonyl Fluoride	97-101	endothelin 1	Homo sapiens	24-28
2254952-1	1990	It is well known that pretreatment with the serine esterase inhibitor phenylmethylsulfonyl fluoride (PMSF) can protect experimental animals from organophosphorus-induced delayed neurotoxicity (OPIDN), presumably by blocking the active site of neurotoxic esterase (NTE) such that binding and "aging" of the neuropathic OP is thwarted.	Phenylmethylsulfonyl Fluoride	70-99	patatin like phospholipase domain containing 6	Gallus gallus	243-262
2254952-1	1990	It is well known that pretreatment with the serine esterase inhibitor phenylmethylsulfonyl fluoride (PMSF) can protect experimental animals from organophosphorus-induced delayed neurotoxicity (OPIDN), presumably by blocking the active site of neurotoxic esterase (NTE) such that binding and "aging" of the neuropathic OP is thwarted.	Phenylmethylsulfonyl Fluoride	70-99	patatin like phospholipase domain containing 6	Gallus gallus	264-267
2254952-1	1990	It is well known that pretreatment with the serine esterase inhibitor phenylmethylsulfonyl fluoride (PMSF) can protect experimental animals from organophosphorus-induced delayed neurotoxicity (OPIDN), presumably by blocking the active site of neurotoxic esterase (NTE) such that binding and "aging" of the neuropathic OP is thwarted.	Phenylmethylsulfonyl Fluoride	101-105	patatin like phospholipase domain containing 6	Gallus gallus	243-262
2254952-1	1990	It is well known that pretreatment with the serine esterase inhibitor phenylmethylsulfonyl fluoride (PMSF) can protect experimental animals from organophosphorus-induced delayed neurotoxicity (OPIDN), presumably by blocking the active site of neurotoxic esterase (NTE) such that binding and "aging" of the neuropathic OP is thwarted.	Phenylmethylsulfonyl Fluoride	101-105	patatin like phospholipase domain containing 6	Gallus gallus	264-267
2194575-6	1990	The level of KC mRNA reached a peak 2 h after thrombin treatment and returned to near control levels by 8 h. Thrombin that was pretreated with phenylmethylsulfonyl fluoride (PMSF) to block proteolytic activity did not stimulate KC gene expression.	Phenylmethylsulfonyl Fluoride	143-172	coagulation factor II	Mus musculus	109-117
2225462-1	1990	Lipoamidase, as determined by lipoyl-p-aminobenzoic acid (L-pABA) hydrolyzing activity, and biotinidase in human serum have similar pH profiles, molecular weights, thermostabilities, and are similarly inhibited by p-hydroxymercuribenzoate and not inhibited by phenylmethylsulfonylfluoride.	Phenylmethylsulfonyl Fluoride	260-288	biotinidase	Homo sapiens	92-103
2288984-5	1990	The temperature optimum of the enzyme activity lies at 37 degrees C. The proteinase is completely inactivated by the specific inhibitors of serine proteinases, diisopropylfluorophosphate and phenylmethylsulfonylfluoride, as well as by the SH-group reagent, p-chloromercuribenzoate.	Phenylmethylsulfonyl Fluoride	191-219	endogenous retrovirus group K member 19, envelope	Homo sapiens	73-83
1697312-4	1990	The inhibitors PMSF, chymostatin, and soybean trypsin inhibitor were most effective in preventing radiolabeled human C3a degradation.	Phenylmethylsulfonyl Fluoride	15-19	complement C3	Homo sapiens	117-120
1697312-7	1990	Chemical cross-linking of radiolabeled human C3a to surface components on the rat mast cells, in the presence of PMSF, revealed one major and two minor bands.	Phenylmethylsulfonyl Fluoride	113-117	complement C3	Homo sapiens	45-48
2194575-6	1990	The level of KC mRNA reached a peak 2 h after thrombin treatment and returned to near control levels by 8 h. Thrombin that was pretreated with phenylmethylsulfonyl fluoride (PMSF) to block proteolytic activity did not stimulate KC gene expression.	Phenylmethylsulfonyl Fluoride	174-178	coagulation factor II	Mus musculus	109-117
1697429-3	1990	The critical factor allowing immunological detection of alpha 2M-bound elastase is the addition of phenyl methyl sulphonyl fluoride (PMSF) to the assay buffer.	Phenylmethylsulfonyl Fluoride	133-137	alpha-2-macroglobulin	Homo sapiens	56-64
2324495-6	1990	Using various protease inhibitors (i.e., PMSF) suggested that the C5a inhibitor is a serine protease.	Phenylmethylsulfonyl Fluoride	41-45	complement C5a receptor 1	Homo sapiens	66-69
2324495-6	1990	Using various protease inhibitors (i.e., PMSF) suggested that the C5a inhibitor is a serine protease.	Phenylmethylsulfonyl Fluoride	41-45	coagulation factor II, thrombin	Homo sapiens	85-100
2310382-7	1990	However, platelets bound approximately twice as many molecules of native enzyme as molecules of phenylmethanesulphonyl fluoride-treated cathepsin G per cell.	Phenylmethylsulfonyl Fluoride	96-127	cathepsin G	Homo sapiens	136-147
2191538-3	1990	Osmotically active "small" Chr A fragments (below 30 kDa) were conspicuous in lysates containing cocktails of leupeptin, pepstatin A, pHMB, PMSF and aprotinin.	Phenylmethylsulfonyl Fluoride	140-144	chromogranin A	Bos taurus	27-32
2200749-8	1990	Elastase induced detachment was sensitive to inhibition by phenyl methyl sulfonyl fluoride (PMSF) and alpha 1-anti-proteinase (alpha 1-AP) and, to a lesser extent by aprotinin; trypsin-induced detachment was ablated by PMSF, alpha 1-AP and soybean trypsin inhibitor (SBTI) but not by 1,10 phenanthroline or EDTA.	Phenylmethylsulfonyl Fluoride	92-96	kunitz trypsin protease inhibitor	Glycine max	248-265
33642398-7	2021	Specificity of the method was verified by thrombin and serine-protease inhibitors N-alpha-((2-naphthylsulfinyl)glycyl)-DL-p-amidinophenylalanylpiperidine and phenylmethanesulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	158-188	coagulation factor II, thrombin	Homo sapiens	55-70
2406364-6	1990	The monocyte-conditioned medium was found to cleave purified CTAP-III into NAP-2 through proteinases that were highly sensitive to PMSF, moderately sensitive to leupeptin and insensitive to EDTA.	Phenylmethylsulfonyl Fluoride	131-135	pro-platelet basic protein	Homo sapiens	61-69
2406364-6	1990	The monocyte-conditioned medium was found to cleave purified CTAP-III into NAP-2 through proteinases that were highly sensitive to PMSF, moderately sensitive to leupeptin and insensitive to EDTA.	Phenylmethylsulfonyl Fluoride	131-135	pro-platelet basic protein	Homo sapiens	75-80
26323600-8	2015	Additionally, we found that MG132 and PMSF, which are inhibitors of proteasomes and serine proteases, respectively, increased the Sit1 protein level.	Phenylmethylsulfonyl Fluoride	38-42	siderophore transporter	Saccharomyces cerevisiae S288C	130-134
22897769-7	2012	The activity of the enzyme was inhibited only by irreversible inhibitors specific for serine proteinases (PMSF and 3,4-dichloro-isocumarine), suggesting that the enzyme was a serine proteinase.	Phenylmethylsulfonyl Fluoride	106-110	endogenous retrovirus group K member 18	Homo sapiens	93-103
2302420-4	1990	Pre-exposure of MOLT-4 lymphoblasts to 1 mM of the serine hydrolase inhibitor phenylmethylsulfonyl fluoride resulted in an inhibition of PAF metabolism.	Phenylmethylsulfonyl Fluoride	78-107	PCNA clamp associated factor	Homo sapiens	137-140
2154953-3	1990	The yield of [3H]PAF could be dramatically increased by pretreating the cells with either oleic acid, an activator of CTP:phosphocholine cytidylyltransferase, or phenylmethylsulfonyl fluoride, an inhibitor of PAF acetylhydrolase.	Phenylmethylsulfonyl Fluoride	162-191	PCNA clamp associated factor	Homo sapiens	17-20
2154953-3	1990	The yield of [3H]PAF could be dramatically increased by pretreating the cells with either oleic acid, an activator of CTP:phosphocholine cytidylyltransferase, or phenylmethylsulfonyl fluoride, an inhibitor of PAF acetylhydrolase.	Phenylmethylsulfonyl Fluoride	162-191	phospholipase A2 group VII	Homo sapiens	209-228
34688831-9	2021	PMF treatment also suppressed the VEGF levels and enhanced the bFGF levels in both neural tissues.	Phenylmethylsulfonyl Fluoride	0-3	vascular endothelial growth factor A	Rattus norvegicus	34-38
34688831-9	2021	PMF treatment also suppressed the VEGF levels and enhanced the bFGF levels in both neural tissues.	Phenylmethylsulfonyl Fluoride	0-3	fibroblast growth factor 2	Rattus norvegicus	63-67
34642230-9	2022	YidC and PMF stimulated Pf3-Lep insertion as the synthesis level increased.	Phenylmethylsulfonyl Fluoride	9-12	leptin	Homo sapiens	28-31
34903990-3	2021	This study was designed to evaluate the effect of phenylmethylsulfonyl fluoride (PMSF), an inhibitor of fatty acid amide hydrolase (FAAH), on morphine antinociceptive tolerance and physical dependence in mice.	Phenylmethylsulfonyl Fluoride	50-79	fatty acid amide hydrolase	Mus musculus	104-130
34903990-3	2021	This study was designed to evaluate the effect of phenylmethylsulfonyl fluoride (PMSF), an inhibitor of fatty acid amide hydrolase (FAAH), on morphine antinociceptive tolerance and physical dependence in mice.	Phenylmethylsulfonyl Fluoride	50-79	fatty acid amide hydrolase	Mus musculus	132-136
34903990-3	2021	This study was designed to evaluate the effect of phenylmethylsulfonyl fluoride (PMSF), an inhibitor of fatty acid amide hydrolase (FAAH), on morphine antinociceptive tolerance and physical dependence in mice.	Phenylmethylsulfonyl Fluoride	81-85	fatty acid amide hydrolase	Mus musculus	104-130
34903990-3	2021	This study was designed to evaluate the effect of phenylmethylsulfonyl fluoride (PMSF), an inhibitor of fatty acid amide hydrolase (FAAH), on morphine antinociceptive tolerance and physical dependence in mice.	Phenylmethylsulfonyl Fluoride	81-85	fatty acid amide hydrolase	Mus musculus	132-136
35563384-5	2022	In the biofilm formation kinetics analysis, the addition of phenylmethylsulfonyl fluoride (PMSF; a proteinase-3 inhibitor) showed that proteinase-3 participates in the cell aggregation stage of biofilm formation.	Phenylmethylsulfonyl Fluoride	60-89	proteinase 3	Mus musculus	99-111
35563384-5	2022	In the biofilm formation kinetics analysis, the addition of phenylmethylsulfonyl fluoride (PMSF; a proteinase-3 inhibitor) showed that proteinase-3 participates in the cell aggregation stage of biofilm formation.	Phenylmethylsulfonyl Fluoride	60-89	proteinase 3	Mus musculus	135-147
35563384-5	2022	In the biofilm formation kinetics analysis, the addition of phenylmethylsulfonyl fluoride (PMSF; a proteinase-3 inhibitor) showed that proteinase-3 participates in the cell aggregation stage of biofilm formation.	Phenylmethylsulfonyl Fluoride	91-95	proteinase 3	Mus musculus	99-111
35563384-5	2022	In the biofilm formation kinetics analysis, the addition of phenylmethylsulfonyl fluoride (PMSF; a proteinase-3 inhibitor) showed that proteinase-3 participates in the cell aggregation stage of biofilm formation.	Phenylmethylsulfonyl Fluoride	91-95	proteinase 3	Mus musculus	135-147
35000560-7	2022	Its activity was enhanced by Cu2+, Na+, Zn2+, and completely inhibited by phenylmethanesulfonyl fluoride, soybean trypsin inhibitor, aprotinin, which indicates it could be a serine protease.	Phenylmethylsulfonyl Fluoride	74-104	subtilisin-like protease C1	Glycine max	174-189
2610022-2	1989	The osteonectin-degrading activity was further characterized and found to be optimally active between pH 6 and 8 and inhibited with EDTA and 1, 10-phenanthroline but not phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	170-199	secreted protein acidic and cysteine rich	Rattus norvegicus	4-15
2507578-9	1989	The serine protease inhibitors, PMSF and alpha 1-antitrypsin prevented the lavage supernatant from reducing CR1 expression, while metalloprotease inhibitors had no effect.	Phenylmethylsulfonyl Fluoride	32-36	complement C3b/C4b receptor 1 (Knops blood group)	Homo sapiens	108-111
2513808-4	1989	Catalytically inactive thrombin (phenylmethanesulphonyl fluoride-treated) had no effect on monocyte adhesion to EC.	Phenylmethylsulfonyl Fluoride	33-64	coagulation factor II, thrombin	Homo sapiens	23-31
2808367-6	1989	Both specific binding activity to the VAI gene and TFIIIC transcription activity were inhibited by the alkylating agents diisopropyl fluorophosphate, N-tosyl-L-phenylalanine chloromethyl ketone (TPCK), and N-ethylmaleimide, and to a lesser extent by N alpha-p-tosyl-L-lysine chloromethyl ketone, whereas neither activity was inhibited by phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	338-367	general transcription factor IIIC subunit 1	Homo sapiens	51-57
2573346-11	1989	Fibronectin appeared to bind DPP IV at a site distinct from its exopeptidase substrate recognition site since protease inhibitors such as competitive peptide substrates and phenylmethanesulphonyl fluoride enhanced binding, possibly as a result of an altered conformation of DPP IV.	Phenylmethylsulfonyl Fluoride	173-204	fibronectin 1	Rattus norvegicus	0-11
2780547-6	1989	Phenylmethylsulfonyl fluoride, a serine enzyme inhibitor, was inhibitory to the expressed CEH activity, whereas p-chloromercuribenzoate (up to 5 mM), a potent thiol-blocking agent, did not significantly inhibit the expressed activity.	Phenylmethylsulfonyl Fluoride	0-29	epoxide hydrolase 2	Rattus norvegicus	90-93
2573346-11	1989	Fibronectin appeared to bind DPP IV at a site distinct from its exopeptidase substrate recognition site since protease inhibitors such as competitive peptide substrates and phenylmethanesulphonyl fluoride enhanced binding, possibly as a result of an altered conformation of DPP IV.	Phenylmethylsulfonyl Fluoride	173-204	dipeptidylpeptidase 4	Rattus norvegicus	29-35
2751751-3	1989	Macrophage conditioned medium performed the modification in the absence of cells, and phenylmethylsulphonyl fluoride (PMSF) inhibited beta-VLDL modification, whereas other protease inhibitors did not, suggesting that a secreted neutral serine protease may possibly be involved in the mechanism.	Phenylmethylsulfonyl Fluoride	86-116	complement component 1, s subcomponent 1	Mus musculus	236-251
2760571-4	1989	Serine protease inhibitors (diisopropylfluorophosphate, phenylmethanesulfonyl fluoride, soybean trypsin inhibitor, aprotinin, benzamidine, and chloromethyl ketones) suppressed ASP activity 22 to 70%.	Phenylmethylsulfonyl Fluoride	56-86	subtilisin-like protease C1	Glycine max	0-15
2745405-9	1989	The proline-beta-naphthylamidase activity was drastically inhibited by diisopropylfluorophosphate, phenylmethanesulfonyl fluoride, and L-1-tosylamido-2-phenylethyl chloromethyl ketone, indicating that the enzyme is a serine hydrolase, whereas it was slightly inhibited by aminopeptidase inhibitors, such as amastatin, bestatin, and puromycin.	Phenylmethylsulfonyl Fluoride	99-129	liver carboxylesterase	Sus scrofa	4-32
2745405-9	1989	The proline-beta-naphthylamidase activity was drastically inhibited by diisopropylfluorophosphate, phenylmethanesulfonyl fluoride, and L-1-tosylamido-2-phenylethyl chloromethyl ketone, indicating that the enzyme is a serine hydrolase, whereas it was slightly inhibited by aminopeptidase inhibitors, such as amastatin, bestatin, and puromycin.	Phenylmethylsulfonyl Fluoride	99-129	alanyl aminopeptidase, membrane	Sus scrofa	272-286
2742884-10	1989	Difference photoaffinity labeling with GSSG, S-hexylglutathione, taurocholate and phenylmethylsulfonyl fluoride decreased the radioactivity bound by GST, but not that introduced into the 105 kDa protein band present in CMV.	Phenylmethylsulfonyl Fluoride	82-111	hematopoietic prostaglandin D synthase	Rattus norvegicus	149-152
2751751-3	1989	Macrophage conditioned medium performed the modification in the absence of cells, and phenylmethylsulphonyl fluoride (PMSF) inhibited beta-VLDL modification, whereas other protease inhibitors did not, suggesting that a secreted neutral serine protease may possibly be involved in the mechanism.	Phenylmethylsulfonyl Fluoride	118-122	complement component 1, s subcomponent 1	Mus musculus	236-251
2519887-6	1989	Pretreatment of neonatal rat myocytes with phenylmethylsulfonyl fluoride partially blocked the deacetylation of [3H]PAF and decreased both the formation and subsequent acylation of [3H]alkyllyso-GPC in intact cells.	Phenylmethylsulfonyl Fluoride	43-72	PCNA clamp associated factor	Rattus norvegicus	116-119
2661415-2	1989	It was observed that the cytotoxic activity of CF was inhibited significantly, in a dose-dependent manner, by pretreatment with bovine pancreatic trypsin inhibitor (BPTI) and phenylmethylsulphonyl fluoride (PMSF), to a lesser extent by soya-bean trypsin inhibitor (SBTI) and leupeptin and not at all by 1,10-phenanthroline (OP).	Phenylmethylsulfonyl Fluoride	175-205	trophoblast Kunitz domain protein 1	Bos taurus	246-263
2661415-2	1989	It was observed that the cytotoxic activity of CF was inhibited significantly, in a dose-dependent manner, by pretreatment with bovine pancreatic trypsin inhibitor (BPTI) and phenylmethylsulphonyl fluoride (PMSF), to a lesser extent by soya-bean trypsin inhibitor (SBTI) and leupeptin and not at all by 1,10-phenanthroline (OP).	Phenylmethylsulfonyl Fluoride	207-211	spleen trypsin inhibitor I	Bos taurus	165-169
2661415-2	1989	It was observed that the cytotoxic activity of CF was inhibited significantly, in a dose-dependent manner, by pretreatment with bovine pancreatic trypsin inhibitor (BPTI) and phenylmethylsulphonyl fluoride (PMSF), to a lesser extent by soya-bean trypsin inhibitor (SBTI) and leupeptin and not at all by 1,10-phenanthroline (OP).	Phenylmethylsulfonyl Fluoride	207-211	trophoblast Kunitz domain protein 1	Bos taurus	246-263
2519887-6	1989	Pretreatment of neonatal rat myocytes with phenylmethylsulfonyl fluoride partially blocked the deacetylation of [3H]PAF and decreased both the formation and subsequent acylation of [3H]alkyllyso-GPC in intact cells.	Phenylmethylsulfonyl Fluoride	43-72	glycophorin C	Rattus norvegicus	195-198
2788857-10	1989	The serine proteinase inhibitor phenylmethylsulfonyl fluoride but not the metalloproteinase inhibitor EDTA prevented alpha 1-AT proteolysis, thus granulocyte elastase can mediate alpha 1-AT degradation in CF.	Phenylmethylsulfonyl Fluoride	32-61	serpin family A member 1	Homo sapiens	117-127
2912501-8	1989	This PTH-degrading activity was strongly inhibited by phenylmethylsulfonyl fluoride and chymostatin, but not by soybean trypsin inhibitor, elastatinal or inhibitors of cysteine, aspartic or metalloproteinases, indicating that it is due to a seryl chymotrypsin-like endopeptidase.	Phenylmethylsulfonyl Fluoride	54-83	parathyroid hormone	Rattus norvegicus	5-8
2788857-10	1989	The serine proteinase inhibitor phenylmethylsulfonyl fluoride but not the metalloproteinase inhibitor EDTA prevented alpha 1-AT proteolysis, thus granulocyte elastase can mediate alpha 1-AT degradation in CF.	Phenylmethylsulfonyl Fluoride	32-61	serpin family A member 1	Homo sapiens	179-189
3186727-3	1988	However, recovery of CCK-ir is enhanced up to 3-fold in the presence of serine-alkylating reagents (i.e., phenylmethylsulfonyl fluoride) as well as selected active site-directed inactivators (i.e., peptide chloromethyl ketones) or transition-state inhibitors (i.e., peptide boronic acids) of serine peptidases.	Phenylmethylsulfonyl Fluoride	106-135	cholecystokinin	Rattus norvegicus	21-24
3378041-2	1988	Chromatography of various domains of the central nodule (desAB-NDSK, fibrinogen E, and fibrin E) having nonidentical amino acid sequences showed that all of these fragments are capable of binding to PMSF-thrombin-Sepharose, suggesting that the thrombin binding site resides within the peptide regions common to all of these fragments: alpha(Gly17-Met51), beta(Val55-Met118), and gamma(Tyr1-Lys53).	Phenylmethylsulfonyl Fluoride	199-203	coagulation factor II, thrombin	Homo sapiens	204-212
3418344-5	1988	However, the half-life of reappearance of active NTE was 2.07 days +/- 0.13 (SD, n = 6) for brain and 3.62 days +/- 0.23 (SD, n = 6) for spinal cord--shorter than after dosing with phenylmethylsulphonyl fluoride.	Phenylmethylsulfonyl Fluoride	181-211	patatin like phospholipase domain containing 6	Gallus gallus	49-52
3403561-8	1988	Fibronectin was isolated from citrated human plasma by sequential gelatin-Sepharose affinity and DEAE ion-exchange chromatography in the presence of buffers containing 1 mM phenylmethylsulfonyl fluoride to prevent fragmentation.	Phenylmethylsulfonyl Fluoride	173-202	fibronectin 1	Homo sapiens	0-11
3171170-4	1988	When the active site of kallikrein was blocked by phenylmethylsulfonyl fluoride or D-Phe-D-Phe-L-Arg-CH2Cl, no complex formation was detected.	Phenylmethylsulfonyl Fluoride	50-79	kallikrein related peptidase 4	Homo sapiens	24-34
2843462-6	1988	While the LIF preparation used in these studies was highly purified, specificity for the LIF effect was demonstrated by the ability of several treatments to prevent augmentation of aggregation including: (1) the competitive binding of LIF to one of its substrates: benzoyl-arginine-ethyl-ester; (2) the blocking of PMN LIF receptors with N-acetyl-D-glucosamine; and (3) phenylmethylsulfonylfluoride treatment of the LIF preparation.	Phenylmethylsulfonyl Fluoride	370-398	LIF interleukin 6 family cytokine	Homo sapiens	10-13
2843462-6	1988	While the LIF preparation used in these studies was highly purified, specificity for the LIF effect was demonstrated by the ability of several treatments to prevent augmentation of aggregation including: (1) the competitive binding of LIF to one of its substrates: benzoyl-arginine-ethyl-ester; (2) the blocking of PMN LIF receptors with N-acetyl-D-glucosamine; and (3) phenylmethylsulfonylfluoride treatment of the LIF preparation.	Phenylmethylsulfonyl Fluoride	370-398	LIF interleukin 6 family cytokine	Homo sapiens	89-92
2843462-6	1988	While the LIF preparation used in these studies was highly purified, specificity for the LIF effect was demonstrated by the ability of several treatments to prevent augmentation of aggregation including: (1) the competitive binding of LIF to one of its substrates: benzoyl-arginine-ethyl-ester; (2) the blocking of PMN LIF receptors with N-acetyl-D-glucosamine; and (3) phenylmethylsulfonylfluoride treatment of the LIF preparation.	Phenylmethylsulfonyl Fluoride	370-398	LIF interleukin 6 family cytokine	Homo sapiens	89-92
2843462-6	1988	While the LIF preparation used in these studies was highly purified, specificity for the LIF effect was demonstrated by the ability of several treatments to prevent augmentation of aggregation including: (1) the competitive binding of LIF to one of its substrates: benzoyl-arginine-ethyl-ester; (2) the blocking of PMN LIF receptors with N-acetyl-D-glucosamine; and (3) phenylmethylsulfonylfluoride treatment of the LIF preparation.	Phenylmethylsulfonyl Fluoride	370-398	LIF interleukin 6 family cytokine	Homo sapiens	89-92
2843462-6	1988	While the LIF preparation used in these studies was highly purified, specificity for the LIF effect was demonstrated by the ability of several treatments to prevent augmentation of aggregation including: (1) the competitive binding of LIF to one of its substrates: benzoyl-arginine-ethyl-ester; (2) the blocking of PMN LIF receptors with N-acetyl-D-glucosamine; and (3) phenylmethylsulfonylfluoride treatment of the LIF preparation.	Phenylmethylsulfonyl Fluoride	370-398	LIF interleukin 6 family cytokine	Homo sapiens	89-92
2965151-6	1988	Addition of phenylmethylsulfonyl fluoride inhibited loss of total CR1 and enhanced the stimulus-induced increases in surface CR1.	Phenylmethylsulfonyl Fluoride	12-41	complement C3b/C4b receptor 1 (Knops blood group)	Homo sapiens	66-69
2965151-6	1988	Addition of phenylmethylsulfonyl fluoride inhibited loss of total CR1 and enhanced the stimulus-induced increases in surface CR1.	Phenylmethylsulfonyl Fluoride	12-41	complement C3b/C4b receptor 1 (Knops blood group)	Homo sapiens	125-128
2448422-8	1988	Degradation of MBP in myelin membranes was partially inhibited by only 5-20% using leupeptin (20 microM) but up to 50% by dithiothreitol mM), phenylmethylsulphonyl fluoride (1 mM), and phosphoramidon (50 microM) but up to 50% by dithiothreitol (DDT, 10 mM).	Phenylmethylsulfonyl Fluoride	142-172	myelin basic protein	Rattus norvegicus	15-18
3378041-4	1988	Chromatography of the isolated chains of fibrinogen E showed that the alpha(Gly17-Lys78) peptide region itself contains a strong binding site for PMSF-thrombin-Sepharose.	Phenylmethylsulfonyl Fluoride	146-150	fibrinogen beta chain	Homo sapiens	41-51
3378041-4	1988	Chromatography of the isolated chains of fibrinogen E showed that the alpha(Gly17-Lys78) peptide region itself contains a strong binding site for PMSF-thrombin-Sepharose.	Phenylmethylsulfonyl Fluoride	146-150	coagulation factor II, thrombin	Homo sapiens	151-159
3193362-4	1988	TNF contained in P. pastoris cell lysates was biologically active as determined by its cytotoxic effect on murine L-929 fibroblast cells and the bioactivity was retained for at least 6 months in the lysates stored frozen at -20 degrees C in the presence of protease inhibitor PMSF.	Phenylmethylsulfonyl Fluoride	276-280	tumor necrosis factor	Homo sapiens	0-3
3275655-5	1988	It hydrolyzes synthetic substrates at carbonyl bonds of Arg or Lys residues, and the hydrolysis is strongly inhibited by diisopropylfluorophosphate, phenylmethanesulfonyl fluoride, and leupeptin, suggesting that it is a trypsin-like protease.	Phenylmethylsulfonyl Fluoride	149-179	trypsin-like protease	Bombyx mori	220-241
2448241-4	1988	Phenylmethylsulfonyl fluoride, a serine protease inhibitor, inhibited both free tonin and the solid-phase antibody-bound tonin-alpha 1-macroglobulin complex.	Phenylmethylsulfonyl Fluoride	0-29	kallikrein 1-related peptidase C2	Rattus norvegicus	80-85
2448241-4	1988	Phenylmethylsulfonyl fluoride, a serine protease inhibitor, inhibited both free tonin and the solid-phase antibody-bound tonin-alpha 1-macroglobulin complex.	Phenylmethylsulfonyl Fluoride	0-29	kallikrein 1-related peptidase C2	Rattus norvegicus	121-126
2448241-4	1988	Phenylmethylsulfonyl fluoride, a serine protease inhibitor, inhibited both free tonin and the solid-phase antibody-bound tonin-alpha 1-macroglobulin complex.	Phenylmethylsulfonyl Fluoride	0-29	pregnancy-zone protein	Rattus norvegicus	127-148
3695806-3	1987	Saturation analysis and Scatchard plots showed that the binding of 17 beta -estradiol to the estrogen receptor (ER) was inhibited by PMSF.	Phenylmethylsulfonyl Fluoride	133-137	estrogen receptor 1	Homo sapiens	93-110
3695806-0	1987	Phenylmethylsulfonyl fluoride (PMSF) inhibits 17 beta-estradiol binding to estrogen receptor from human prostate.	Phenylmethylsulfonyl Fluoride	0-29	estrogen receptor 1	Homo sapiens	75-92
3695806-3	1987	Saturation analysis and Scatchard plots showed that the binding of 17 beta -estradiol to the estrogen receptor (ER) was inhibited by PMSF.	Phenylmethylsulfonyl Fluoride	133-137	estrogen receptor 1	Homo sapiens	112-114
3695806-0	1987	Phenylmethylsulfonyl fluoride (PMSF) inhibits 17 beta-estradiol binding to estrogen receptor from human prostate.	Phenylmethylsulfonyl Fluoride	31-35	estrogen receptor 1	Homo sapiens	75-92
3695806-8	1987	Our study indicates that PMSF significantly affects 17 beta -estradiol binding to ER and consequently alters the estimation of ER in Human BPH.	Phenylmethylsulfonyl Fluoride	25-29	estrogen receptor 1	Homo sapiens	82-84
3695806-8	1987	Our study indicates that PMSF significantly affects 17 beta -estradiol binding to ER and consequently alters the estimation of ER in Human BPH.	Phenylmethylsulfonyl Fluoride	25-29	estrogen receptor 1	Homo sapiens	127-129
3498759-6	1987	PMSF inactivation of BLT esterase in detergent extracts of CTL diminished sharply as the pH was dropped below 7.	Phenylmethylsulfonyl Fluoride	0-4	granzyme A	Mus musculus	21-33
2444671-4	1987	By contrast, phenylmethylsulfonyl fluoride (30 mg/kg s.c.), an agent that prevents the development of OP neuropathy by inhibiting NTE without the "aging" reaction, had no effect on axon transport, nerve fiber integrity, or clinical status and, when administered prior to a neurotoxic dose of DBDCVP (1.00 mg/kg s.c.), prevented DBDCVP effects.	Phenylmethylsulfonyl Fluoride	13-42	patatin like phospholipase domain containing 6	Gallus gallus	130-133
3498759-9	1987	In parallel studies of inactivation of CTL lytic activity, PMSF pretreatment caused a 50% reduction of the lytic activity under conditions where greater than 90% of the BLT esterase activity was inactivated.	Phenylmethylsulfonyl Fluoride	59-63	granzyme A	Mus musculus	169-181
3624272-5	1987	The amidolytic and proteolytic activities of the enzyme were readily inhibited by phenylmethanesulfonyl fluoride, p-amidinophenylmethanesulfonyl fluoride, chloromethyl ketones, and human antithrombin III.	Phenylmethylsulfonyl Fluoride	82-112	serpin family C member 1	Homo sapiens	187-203
2888488-6	1987	The pretreatment with 2 mM phenylmethylsulfonyl fluoride (PMSF), a serine proteinase inhibitor reported to block the acetylhydrolase, induced about 2-times more PAF-acether production in response to 2.5 U/ml thrombin stimulation.	Phenylmethylsulfonyl Fluoride	27-56	coagulation factor II, thrombin	Homo sapiens	208-216
2888488-6	1987	The pretreatment with 2 mM phenylmethylsulfonyl fluoride (PMSF), a serine proteinase inhibitor reported to block the acetylhydrolase, induced about 2-times more PAF-acether production in response to 2.5 U/ml thrombin stimulation.	Phenylmethylsulfonyl Fluoride	58-62	coagulation factor II, thrombin	Homo sapiens	208-216
3624867-5	1987	From the following results, we concluded that spontaneous activation may be partially due to proteolytic enzymes contaminating the preparation: 1) a nonspecific protease inhibitor, PMSF, completely inhibited spontaneous activation but did not inhibit the activation of C1 by immune complexes; 2) alpha 2-macroglobulin partially inhibited spontaneous activation, and 3) although spontaneous activation in the absence of PMSF was relatively slow, activated C1 accelerated spontaneous activation that was completely blocked by C1-INH.	Phenylmethylsulfonyl Fluoride	181-185	alpha-2-macroglobulin	Homo sapiens	296-317
3624867-5	1987	From the following results, we concluded that spontaneous activation may be partially due to proteolytic enzymes contaminating the preparation: 1) a nonspecific protease inhibitor, PMSF, completely inhibited spontaneous activation but did not inhibit the activation of C1 by immune complexes; 2) alpha 2-macroglobulin partially inhibited spontaneous activation, and 3) although spontaneous activation in the absence of PMSF was relatively slow, activated C1 accelerated spontaneous activation that was completely blocked by C1-INH.	Phenylmethylsulfonyl Fluoride	181-185	serpin family G member 1	Homo sapiens	524-530
3653270-2	1987	Cathepsin B-like activity was totally inhibited by the specific inhibitor lepeptin (0.1 mM); while DPP-II-like activity was partially inhibited by Tris (10 mM) or puromycin (0.1 mM), which are specific for DPP II, and by phenylmethylsulphonyl fluoride (PMS-F), an inhibitor of serine class proteases.	Phenylmethylsulfonyl Fluoride	221-251	cathepsin B	Rattus norvegicus	0-11
3040108-2	1987	TCDD binding to the Ah receptor was inhibited by serine proteinase inhibitors phenylmethylsulfonyl fluoride (PMSF), tosyl-lysine chloromethyl ketone (TosLysCH2Cl), tosylamide-phenylethyl chloromethyl ketone (TosPheCH2Cl) and substrates tosyl-L-arginine methyl ester (TosArgOMe) and D-tryptophan methyl ester (TrpOMe).	Phenylmethylsulfonyl Fluoride	78-107	aryl hydrocarbon receptor	Rattus norvegicus	20-31
3040108-2	1987	TCDD binding to the Ah receptor was inhibited by serine proteinase inhibitors phenylmethylsulfonyl fluoride (PMSF), tosyl-lysine chloromethyl ketone (TosLysCH2Cl), tosylamide-phenylethyl chloromethyl ketone (TosPheCH2Cl) and substrates tosyl-L-arginine methyl ester (TosArgOMe) and D-tryptophan methyl ester (TrpOMe).	Phenylmethylsulfonyl Fluoride	109-113	aryl hydrocarbon receptor	Rattus norvegicus	20-31
3653270-2	1987	Cathepsin B-like activity was totally inhibited by the specific inhibitor lepeptin (0.1 mM); while DPP-II-like activity was partially inhibited by Tris (10 mM) or puromycin (0.1 mM), which are specific for DPP II, and by phenylmethylsulphonyl fluoride (PMS-F), an inhibitor of serine class proteases.	Phenylmethylsulfonyl Fluoride	221-251	dipeptidylpeptidase 7	Rattus norvegicus	99-105
3106343-1	1987	The serine protease inhibitors diethyl p-nitrophenyl phosphate and phenylmethylsulfonyl fluoride (chemical modifiers of serine residue) and N-acetyl-l-tryptophan ethyl ester (competitive inhibitor of chymotryptic protease) inhibited 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (AGEPC; platelet-activating factor)-induced platelet aggregation and secretion.	Phenylmethylsulfonyl Fluoride	67-96	serine protease HTRA1	Oryctolagus cuniculus	4-19
3034297-4	1987	We ascertained that the active site of the enzyme was necessary for binding to the chondrocyte, since phenylmethylsulfonyl fluoride-inactivated leukocyte elastase failed to bind.	Phenylmethylsulfonyl Fluoride	102-131	elastase, neutrophil expressed	Homo sapiens	144-162
3494077-4	1987	Both antigen-specific GEF and nonspecific GEF are inactivated by phenylmethylsulfonyl fluoride, but not by N-alpha-p-tosyl-L-lysyl-chloromethyl ketone.	Phenylmethylsulfonyl Fluoride	65-94	rho/rac guanine nucleotide exchange factor (GEF) 2	Mus musculus	22-25
3494077-4	1987	Both antigen-specific GEF and nonspecific GEF are inactivated by phenylmethylsulfonyl fluoride, but not by N-alpha-p-tosyl-L-lysyl-chloromethyl ketone.	Phenylmethylsulfonyl Fluoride	65-94	rho/rac guanine nucleotide exchange factor (GEF) 2	Mus musculus	42-45
3593282-5	1987	Also, the elution profile of phenylmethane-sulphonyl fluoride-inhibited thrombin from fibrinogen-Sepharose was identical with that of active thrombin from fibrin-monomer-Sepharose.	Phenylmethylsulfonyl Fluoride	29-61	coagulation factor II, thrombin	Homo sapiens	72-80
3564033-3	1987	Phenylmethanesulfonyl fluoride (PMSF) also attacks the active center of NTE but "aging" cannot occur.	Phenylmethylsulfonyl Fluoride	0-30	patatin like phospholipase domain containing 6	Gallus gallus	72-75
3564033-3	1987	Phenylmethanesulfonyl fluoride (PMSF) also attacks the active center of NTE but "aging" cannot occur.	Phenylmethylsulfonyl Fluoride	32-36	patatin like phospholipase domain containing 6	Gallus gallus	72-75
3564033-7	1987	PMSF (0.55 mg/kg) injected into each sciatic artery caused 47% inhibition of sciatic nerve NTE but only 17-22% inhibition of NTE elsewhere; it did not produce clinical or histopathological lesions.	Phenylmethylsulfonyl Fluoride	0-4	patatin like phospholipase domain containing 6	Gallus gallus	91-94
3564033-7	1987	PMSF (0.55 mg/kg) injected into each sciatic artery caused 47% inhibition of sciatic nerve NTE but only 17-22% inhibition of NTE elsewhere; it did not produce clinical or histopathological lesions.	Phenylmethylsulfonyl Fluoride	0-4	patatin like phospholipase domain containing 6	Gallus gallus	125-128
3564033-9	1987	PMSF (1 mg/kg) injected into only one sciatic artery caused selective protective inhibition of sciatic nerve NTE of that leg.	Phenylmethylsulfonyl Fluoride	0-4	patatin like phospholipase domain containing 6	Gallus gallus	109-112
3593282-5	1987	Also, the elution profile of phenylmethane-sulphonyl fluoride-inhibited thrombin from fibrinogen-Sepharose was identical with that of active thrombin from fibrin-monomer-Sepharose.	Phenylmethylsulfonyl Fluoride	29-61	fibrinogen beta chain	Homo sapiens	86-96
3115874-1	1987	Preincubation of human monocytes with different amounts of human lymphokine at 37 degrees C dose dependently increased the uptake of EA cells at both 37 degrees C and 4 degrees C. Phenylmethanesulphonyl fluoride (PMSF), an inhibitor of serine esterases, inhibited the process.	Phenylmethylsulfonyl Fluoride	180-211	interleukin 2	Homo sapiens	65-75
3479089-3	1987	Sediment (Sed) inactivated functionally pure C4 (fourth component of complement), and this action on C4 was inhibited by EDTA, phenylmethylsulphonylfluoride (PMSF, a serine-esterase inhibitor) and C1-inhibitor (C1-In).	Phenylmethylsulfonyl Fluoride	158-162	serpin family G member 1	Homo sapiens	197-209
3479089-3	1987	Sediment (Sed) inactivated functionally pure C4 (fourth component of complement), and this action on C4 was inhibited by EDTA, phenylmethylsulphonylfluoride (PMSF, a serine-esterase inhibitor) and C1-inhibitor (C1-In).	Phenylmethylsulfonyl Fluoride	158-162	serpin family G member 1	Homo sapiens	211-216
3115874-1	1987	Preincubation of human monocytes with different amounts of human lymphokine at 37 degrees C dose dependently increased the uptake of EA cells at both 37 degrees C and 4 degrees C. Phenylmethanesulphonyl fluoride (PMSF), an inhibitor of serine esterases, inhibited the process.	Phenylmethylsulfonyl Fluoride	213-217	interleukin 2	Homo sapiens	65-75
3464972-10	1986	The biological activity present in the neutrophil supernatant was destroyed by heat and inactivated by treatment with phenylmethylsulfonyl fluoride, indicating that it is a protein and most probably an enzyme with serine protease activity.	Phenylmethylsulfonyl Fluoride	118-147	coagulation factor II, thrombin	Homo sapiens	214-229
3700425-11	1986	Cholesterol arachidonate was formed upon incubation of phenylmethanesulfonyl fluoride-modified LCAT with arachidonyl-CoA.	Phenylmethylsulfonyl Fluoride	55-85	lecithin-cholesterol acyltransferase	Homo sapiens	95-99
2948527-2	1986	Lp(a) was ultracentrifuged through a layer of saline which was adjusted to a density of 1.182 g/mL and contained 30 mM dithiothreitol (50 mM) and phenylmethylsulfonyl fluoride (1.25 mM).	Phenylmethylsulfonyl Fluoride	146-175	lipoprotein(a)	Homo sapiens	0-5
3766969-4	1986	Human thrombin was more reactive than bovine thrombin with these active site-directed probes: phenylmethylsulfonyl fluoride, dansyl-Glu-Gly-Arg-chloromethylketone, and human heparin cofactor II.	Phenylmethylsulfonyl Fluoride	94-123	coagulation factor II, thrombin	Homo sapiens	6-14
3700425-7	1986	Lecithin alone protected the phospholipase A2 activity against phenylmethanesulfonyl fluoride inactivation but not the transacylase against 5,5"-dithiobis-(2-nitrobenzoic acid) inactivation.	Phenylmethylsulfonyl Fluoride	63-93	phospholipase A2 group IB	Homo sapiens	29-45
3633949-4	1986	Both antibodies, named 2E9.8 and 2E9.9, bound active 125I-kallikrein and phenylmethylsulfonyl fluoride (PMSF)-inactivated 125I-kallikrein.	Phenylmethylsulfonyl Fluoride	73-102	kallikrein related peptidase 4	Homo sapiens	127-137
3486653-9	1986	Although the prototypic serine protease inhibitor phenyl methylsulfonylfluoride (PMSF) protected endothelium from PMNs, pure alpha-1-PI (also a potent anti-elastase) when added in physiologic amounts did not protect endothelial cells from PMN assault, suggesting that PMN oxidants might inactivate it.	Phenylmethylsulfonyl Fluoride	81-85	serpin family A member 1	Homo sapiens	125-135
3633949-4	1986	Both antibodies, named 2E9.8 and 2E9.9, bound active 125I-kallikrein and phenylmethylsulfonyl fluoride (PMSF)-inactivated 125I-kallikrein.	Phenylmethylsulfonyl Fluoride	104-108	kallikrein related peptidase 4	Homo sapiens	127-137
3544714-3	1986	Inactivation of kallikrein by PMSF or inhibition with aprotinin blocked kallikrein stimulation of renin release.	Phenylmethylsulfonyl Fluoride	30-34	renin	Rattus norvegicus	98-103
3080438-7	1986	Melittin disruption of vinculin was inhibited by (in order of decreasing effectiveness) mepacrine greater than TMB-8 greater than TFP greater than leupeptin greater than PMSF, whereas A23187 and amiloride had no effect.	Phenylmethylsulfonyl Fluoride	170-174	vinculin	Mus musculus	23-31
3931945-4	1985	The serine protease inhibitor PMSF destroyed LIF activity at concentrations of 10(-2) to 10(-3) M. Concanavalin A stimulated production of detectable levels of LIF by 8 hr, while SEA and SWAP did so by 15 and 39 hr, respectively.	Phenylmethylsulfonyl Fluoride	30-34	LIF interleukin 6 family cytokine	Homo sapiens	45-48
3931945-4	1985	The serine protease inhibitor PMSF destroyed LIF activity at concentrations of 10(-2) to 10(-3) M. Concanavalin A stimulated production of detectable levels of LIF by 8 hr, while SEA and SWAP did so by 15 and 39 hr, respectively.	Phenylmethylsulfonyl Fluoride	30-34	LIF interleukin 6 family cytokine	Homo sapiens	160-163
4084237-1	1985	Thrombin treated with phenylmethanesulphonyl fluoride, like active enzyme, promotes modifications to human platelet cytoskeleton.	Phenylmethylsulfonyl Fluoride	22-53	coagulation factor II, thrombin	Homo sapiens	0-8
3876772-3	1985	Use of glycerol and the protease inhibitor phenylmethylsulfonyl fluoride resulted in a fourfold increase in the yield of androgen receptor from human neonatal foreskin and a tenfold increase in stability of this receptor.	Phenylmethylsulfonyl Fluoride	43-72	androgen receptor	Homo sapiens	121-138
4052028-15	1985	In contrast, 10-12 times more PAF is produced when platelets are treated with PMSF and stimulated with thrombin.	Phenylmethylsulfonyl Fluoride	78-82	PCNA clamp associated factor	Homo sapiens	30-33
4052028-17	1985	In addition, these platelets (treated with PMSF and stimulated with thrombin) incorporate exogenous labelled acetate in the 2-position of PAF, probably by an acetyltransferase-dependent mechanism.	Phenylmethylsulfonyl Fluoride	43-47	PCNA clamp associated factor	Homo sapiens	138-141
4052028-18	1985	Production of PAF by human platelets during physiological stimulation can be demonstrated when PAF degradation is suppressed by the acetyl-hydrolase inhibitor PMSF.	Phenylmethylsulfonyl Fluoride	159-163	PCNA clamp associated factor	Homo sapiens	14-17
4052028-18	1985	Production of PAF by human platelets during physiological stimulation can be demonstrated when PAF degradation is suppressed by the acetyl-hydrolase inhibitor PMSF.	Phenylmethylsulfonyl Fluoride	159-163	PCNA clamp associated factor	Homo sapiens	95-98
3888276-10	1985	All purified lecithin-cholesterol acyltransferase species were activated by human apolipoprotein A-I and were similarly inhibited by p-hydroxymercuribenzoate and phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	162-191	lecithin-cholesterol acyltransferase	Homo sapiens	13-49
2417348-3	1985	The effect of thrombin was fully inhibited by the presence of 50% (v/v) fetal calf serum or more in the medium, by preincubation of thrombin with hirudin or by treatment of thrombin with N-bromosuccinimide or phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	209-238	coagulation factor II, thrombin	Homo sapiens	14-22
2986734-4	1985	The total cytochrome c hydrolase activity of these enzymes was inhibited by phenylmethylsulfonylfluoride but was insensitive to ethylenediaminetetraacetate and o-phenanthroline.	Phenylmethylsulfonyl Fluoride	76-104	cytochrome c, somatic	Homo sapiens	10-22
2985563-3	1985	Evidence for this includes a pH optimum of 5.0 for the first order constant of ubiquitin inactivation and observation that inactivation is inhibited by EDTA, o-phenanthroline, iodoacetamide, p-chloromercuribenzoic acid, phenylmethylsulfonyl fluoride, N alpha-p-tosyl-L-lysine chloromethyl ketone, leupeptin, soybean trypsin inhibitor, and aprotinin.	Phenylmethylsulfonyl Fluoride	220-249	polyubiquitin	Glycine max	79-88
6394762-4	1984	TIF activity was resistant to treatment with trypsin, chymotrypsin and neuraminidase but sensitive to PMSF (phenyl-methyl-sulfonyl-fluoride).	Phenylmethylsulfonyl Fluoride	102-106	TYRO3 protein tyrosine kinase	Homo sapiens	0-3
2408793-4	1985	Enkephalin immunoreactivity paralleled the enkephalin assay standard curves and was not abolished by boiling or by protease inhibitors (EDTA, PMSF).	Phenylmethylsulfonyl Fluoride	142-146	proenkephalin	Rattus norvegicus	0-10
6487368-5	1984	The level of NTE inhibition after PMSF was greater than 40% in the sciatic nerve on the injected side compared with less than 20% in other parts of the nervous system.	Phenylmethylsulfonyl Fluoride	34-38	patatin like phospholipase domain containing 6	Gallus gallus	13-16
6487368-6	1984	The same dose of PMSF injected in the wing vein produced low NTE inhibition in the nervous system and failed to protect the animals from the same high systemic dose of DFP.	Phenylmethylsulfonyl Fluoride	17-21	patatin like phospholipase domain containing 6	Gallus gallus	61-64
6236547-5	1984	The cleavage of C3 and the covalent fixation of C3b are shown to be inhibited by phenylmethylsulphonyl fluoride and methylamine, respectively.	Phenylmethylsulfonyl Fluoride	81-111	complement C3	Homo sapiens	48-51
6207774-7	1984	Under comparable conditions, somewhat less alpha 2M-trypsin precipitated from solutions containing H3 than did alpha 2M-methylamine; however, inactivation of the alpha 2M-trypsin with phenylmethylsulfonyl fluoride prior to incubation increased the level of precipitation significantly.	Phenylmethylsulfonyl Fluoride	184-213	alpha-2-macroglobulin	Homo sapiens	43-51
6372498-5	1984	Generation and the limited proteolysis were both inhibited by phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, suggesting the presence and the role of a serine protease in the fraction.	Phenylmethylsulfonyl Fluoride	62-91	LOW QUALITY PROTEIN: serine protease HTRA1	Cavia porcellus	102-117
6326847-6	1984	Hydroxyproline solubilization was almost totally inhibited by phenylmethylsulphonyl fluoride, indicating that the neutrophil serine proteinases, elastase and cathepsin G are responsible for degradation.	Phenylmethylsulfonyl Fluoride	62-92	cathepsin G	Homo sapiens	158-169
6428285-4	1984	With the synthetic elastin-like chromophore substrate succinyl-trialanine-nitroanilide ( SLAPN ), activity in individual samples was reduced 79% by EDTA, a metalloproteinase inhibitor, whereas activity was reduced by only 29% in the presence of PMSF, a serine proteinase inhibitor.	Phenylmethylsulfonyl Fluoride	245-249	elastin	Homo sapiens	19-26
6428285-5	1984	In addition, using a pooled sample of HLF and C elastin substrate, 80% of activity against the elastin substrate was eliminated by EDTA, whereas 51% was eliminated by PMSF.	Phenylmethylsulfonyl Fluoride	167-171	HLF transcription factor, PAR bZIP family member	Homo sapiens	38-41
6330984-2	1984	Proteolysis is blocked by diisopropyl fluorophosphonate (DFP) and phenylmethyl sulfonyl fluoride (PMSF), suggesting that the virion-associated enzyme is a serine protease.	Phenylmethylsulfonyl Fluoride	66-96	coagulation factor II, thrombin	Homo sapiens	155-170
6330984-2	1984	Proteolysis is blocked by diisopropyl fluorophosphonate (DFP) and phenylmethyl sulfonyl fluoride (PMSF), suggesting that the virion-associated enzyme is a serine protease.	Phenylmethylsulfonyl Fluoride	98-102	coagulation factor II, thrombin	Homo sapiens	155-170
2663127-11	1989	Similar to PRB, it could be inhibited by up to 90% with phenylmethylsulfonyl fluoride and para-chloromercuribenzoate and preferentially hydrolyzed the Leu15-Tyr16 peptide bond of the oxidized beta-chain of insulin.	Phenylmethylsulfonyl Fluoride	56-85	proteinase B	Saccharomyces cerevisiae S288C	11-14
6378180-9	1984	The enzyme was inactivated by phenylmethanesulphonyl fluoride and was thus classified as a serine proteinase.	Phenylmethylsulfonyl Fluoride	30-61	endogenous retrovirus group K member 18	Homo sapiens	98-108
6372498-5	1984	Generation and the limited proteolysis were both inhibited by phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, suggesting the presence and the role of a serine protease in the fraction.	Phenylmethylsulfonyl Fluoride	62-91	LOW QUALITY PROTEIN: serine protease HTRA1	Cavia porcellus	171-186
6372498-5	1984	Generation and the limited proteolysis were both inhibited by phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, suggesting the presence and the role of a serine protease in the fraction.	Phenylmethylsulfonyl Fluoride	93-97	LOW QUALITY PROTEIN: serine protease HTRA1	Cavia porcellus	102-117
6372498-5	1984	Generation and the limited proteolysis were both inhibited by phenylmethylsulfonyl fluoride (PMSF), a serine protease inhibitor, suggesting the presence and the role of a serine protease in the fraction.	Phenylmethylsulfonyl Fluoride	93-97	LOW QUALITY PROTEIN: serine protease HTRA1	Cavia porcellus	171-186
6408078-10	1983	The in vitro cleavage event in extracts of lon+ CSH-10 or the isogenic lon- mutant is not stimulated by addition of ATP, not inhibited by depletion of ATP pools by hexokinase-2-deoxyglucose treatment, and not inhibited by EDTA or phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	230-259	putative ATP-dependent Lon protease	Escherichia coli	43-46
6706971-3	1984	The thioesterase component of chicken liver fatty acid synthetase was either inhibited using phenylmethanesulfonyl fluoride or diisopropyl fluorophosphate, or released from the synthetase by limited proteolysis with alpha-chymotrypsin, thus ensuring that the fatty acyl products remain bound to the enzyme.	Phenylmethylsulfonyl Fluoride	93-123	fatty acid synthase	Gallus gallus	4-16
24173451-4	1983	The ski5 mutation caused an approximate ten fold oversecretion of toxin, similar to that seen in a PMSF-treated wild type culture, and no increased oversecretion in the presence of PMSF.	Phenylmethylsulfonyl Fluoride	99-103	SKI complex subunit tetratricopeptide repeat protein SKI3	Saccharomyces cerevisiae S288C	4-8
6408058-5	1983	When the enzyme was purified at pH 6.0 in the presence of the covalent inhibitor phenylmethylsulfonyl fluoride, using the rabbit antiserum to detect the bacillopeptidase F protein, no fast-moving electrophoretic forms were observed.	Phenylmethylsulfonyl Fluoride	81-110	bacillopeptidase F	Bacillus subtilis subsp. subtilis str. 168	153-171
6319529-7	1984	On both cells, binding of Fn-ms was inhibited by sufficient concentrations of fluid-phase Fn; both PMN and monocytes bound fewer Fn-ms at 4 degrees C than at 37 degrees C; both achieved maximal binding at similar Fn-ms/cell ratios; and phenylmethylsulfonyl fluoride did not inhibit Fn-ms binding to either cell type.	Phenylmethylsulfonyl Fluoride	236-265	fibronectin 1	Homo sapiens	26-28
6408078-10	1983	The in vitro cleavage event in extracts of lon+ CSH-10 or the isogenic lon- mutant is not stimulated by addition of ATP, not inhibited by depletion of ATP pools by hexokinase-2-deoxyglucose treatment, and not inhibited by EDTA or phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	230-259	putative ATP-dependent Lon protease	Escherichia coli	71-74
6601516-3	1983	HSF did not appear to have a serine group(s) in its "active" site since its biologic activity remained intact following treatment with an irreversible serine esterase inhibitor (phenylmethylsulfonyl fluoride).	Phenylmethylsulfonyl Fluoride	178-207	interleukin 6	Homo sapiens	0-3
6871315-4	1983	The presence of a protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), was required during the immunoprecipitation procedure to prevent the proteolytic degradation of testicular ceruloplasmin.	Phenylmethylsulfonyl Fluoride	38-67	ceruloplasmin	Homo sapiens	183-196
6871315-4	1983	The presence of a protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), was required during the immunoprecipitation procedure to prevent the proteolytic degradation of testicular ceruloplasmin.	Phenylmethylsulfonyl Fluoride	69-73	ceruloplasmin	Homo sapiens	183-196
6603572-7	1983	Phenylmethylsulfonyl fluoride (PMSF) and the leukocyte elastase inhibitor, methoxy-succinate-alanine-alanine-valine-chloromethyl ketone (MeO) each inhibited cleavage of C3b by 90% and C3bi by 60%.	Phenylmethylsulfonyl Fluoride	0-29	complement C3	Homo sapiens	169-172
6603572-13	1983	PMN-mediated cleavage of C3b was also inhibited when PMSF- and MeO-treated PMN were washed to remove the fluid phase phase protease inhibitor before adding EAC43b.	Phenylmethylsulfonyl Fluoride	53-57	complement C3	Homo sapiens	25-28
7159570-3	1982	The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol hydrolysis and PNPB hydrolysis activities coelute from the heparin-Sepharose affinity column used to purify LpL and (b) LpL-catalyzed hydrolyses of trioleoylglycerol and PNPB are inhibited to equal extents by phenylmethanesulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	373-403	lipoprotein lipase	Bos taurus	272-275
6826237-1	1983	Incorporation of [3H]-thymidine - [3H]-TdR - into concanavalin A (Con A) stimulated murine splenocytes and thymocytes was found to be enhanced by addition of certain concentrations of phenyl-methylsulfonylfluoride (PMSF), di-isopropylfluorophosphate (DFP), N-alpha-tosyl-L-lysyl-L-chloromethylketone (TLCK), and soybean trypsin inhibitor (SBTI).	Phenylmethylsulfonyl Fluoride	184-213	kunitz trypsin protease inhibitor	Glycine max	320-337
7159570-3	1982	The same protein and same active site are involved in hydrolysis of water-soluble p-nitrophenyl esters and emulsified trioleoylglycerol since (a) trioleoylglycerol hydrolysis and PNPB hydrolysis activities coelute from the heparin-Sepharose affinity column used to purify LpL and (b) LpL-catalyzed hydrolyses of trioleoylglycerol and PNPB are inhibited to equal extents by phenylmethanesulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	373-403	lipoprotein lipase	Bos taurus	284-287
7179206-4	1982	EC exposed to thrombin pretreated with N-bromosuccinimide (modifying the macromolecular site) or phenylmethylsulfonyl fluoride (blocking the serine site) retained normal morphology and did not leak excess amounts of 51Cr.	Phenylmethylsulfonyl Fluoride	97-126	coagulation factor II, thrombin	Homo sapiens	14-22
6294899-3	1982	The interaction between GP Ib and thrombin was abolished when thrombin was blocked either at the active serine site with tosyl-lysine-chloromethyl-ketone (TLCK) or phenylmethylsulfonylfluoride (PMSF) or at the fibrinogen binding site (macromolecular binding site) with N-bromosuccinimide (NBS) or heparin, indicating that both sites have to be freely accessible for the retention of the glycocalicin-related protein by thrombin.	Phenylmethylsulfonyl Fluoride	194-198	coagulation factor II, thrombin	Homo sapiens	62-70
6294899-3	1982	The interaction between GP Ib and thrombin was abolished when thrombin was blocked either at the active serine site with tosyl-lysine-chloromethyl-ketone (TLCK) or phenylmethylsulfonylfluoride (PMSF) or at the fibrinogen binding site (macromolecular binding site) with N-bromosuccinimide (NBS) or heparin, indicating that both sites have to be freely accessible for the retention of the glycocalicin-related protein by thrombin.	Phenylmethylsulfonyl Fluoride	194-198	coagulation factor II, thrombin	Homo sapiens	62-70
7052135-2	1982	The degradation can be partially prevented by addition of proteinase B inhibitor 2 or phenylmethylsulfonyl fluoride, an inhibitor of proteinase B and carboxypeptidase Y.	Phenylmethylsulfonyl Fluoride	86-115	proteinase B	Saccharomyces cerevisiae S288C	133-145
6294899-3	1982	The interaction between GP Ib and thrombin was abolished when thrombin was blocked either at the active serine site with tosyl-lysine-chloromethyl-ketone (TLCK) or phenylmethylsulfonylfluoride (PMSF) or at the fibrinogen binding site (macromolecular binding site) with N-bromosuccinimide (NBS) or heparin, indicating that both sites have to be freely accessible for the retention of the glycocalicin-related protein by thrombin.	Phenylmethylsulfonyl Fluoride	164-192	coagulation factor II, thrombin	Homo sapiens	34-42
6294899-3	1982	The interaction between GP Ib and thrombin was abolished when thrombin was blocked either at the active serine site with tosyl-lysine-chloromethyl-ketone (TLCK) or phenylmethylsulfonylfluoride (PMSF) or at the fibrinogen binding site (macromolecular binding site) with N-bromosuccinimide (NBS) or heparin, indicating that both sites have to be freely accessible for the retention of the glycocalicin-related protein by thrombin.	Phenylmethylsulfonyl Fluoride	164-192	coagulation factor II, thrombin	Homo sapiens	62-70
6294899-3	1982	The interaction between GP Ib and thrombin was abolished when thrombin was blocked either at the active serine site with tosyl-lysine-chloromethyl-ketone (TLCK) or phenylmethylsulfonylfluoride (PMSF) or at the fibrinogen binding site (macromolecular binding site) with N-bromosuccinimide (NBS) or heparin, indicating that both sites have to be freely accessible for the retention of the glycocalicin-related protein by thrombin.	Phenylmethylsulfonyl Fluoride	164-192	coagulation factor II, thrombin	Homo sapiens	62-70
6294899-3	1982	The interaction between GP Ib and thrombin was abolished when thrombin was blocked either at the active serine site with tosyl-lysine-chloromethyl-ketone (TLCK) or phenylmethylsulfonylfluoride (PMSF) or at the fibrinogen binding site (macromolecular binding site) with N-bromosuccinimide (NBS) or heparin, indicating that both sites have to be freely accessible for the retention of the glycocalicin-related protein by thrombin.	Phenylmethylsulfonyl Fluoride	194-198	coagulation factor II, thrombin	Homo sapiens	34-42
7093287-3	1982	Isolation of this species of cytochrome b5 in its native form from microsomes by means of detergent solubilization required the inclusion of the protease inhibitor, phenylmethylsulfonyl fluoride, throughout the isolation steps.	Phenylmethylsulfonyl Fluoride	165-194	cytochrome b5 type A	Rattus norvegicus	29-42
6177714-4	1982	Inhibitors of medullasin such as phenylmethylsulfonyl fluoride and elastatinal prevented the activation of natural cytotoxicity.	Phenylmethylsulfonyl Fluoride	33-62	elastase, neutrophil expressed	Homo sapiens	14-24
6798119-2	1982	Addition of DFP or PMSF (1 X 10(-3) M to 1 X 10(-5) M) showed a dose-dependent inhibitory effect on TRF-induced IgG induction in CESS cells.	Phenylmethylsulfonyl Fluoride	19-23	interleukin 5	Homo sapiens	100-103
6177649-8	1982	A trypsin-normal alpha 2M complex (Tr-N alpha 2M) and PBA activity which was inhibited by PMSF or aprotinin but not by SBTI.	Phenylmethylsulfonyl Fluoride	90-94	alpha-2-macroglobulin	Homo sapiens	17-25
6460618-6	1982	Incubation of these vesicles at room temperature activated (4--10-fold) the ATPase through a process that is partially sensitive to phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	132-161	dynein axonemal heavy chain 8	Homo sapiens	76-82
6177649-8	1982	A trypsin-normal alpha 2M complex (Tr-N alpha 2M) and PBA activity which was inhibited by PMSF or aprotinin but not by SBTI.	Phenylmethylsulfonyl Fluoride	90-94	alpha-2-macroglobulin	Homo sapiens	40-48
6177649-9	1982	The PBA associated with Pt-alpha 2M was also inhibited by PMSF or aprotinin but not by SBTI.	Phenylmethylsulfonyl Fluoride	58-62	alpha-2-macroglobulin	Homo sapiens	27-35
7314260-10	1981	Sucrose gradient centrifugation showed that cytoplasmic ER of colorectal cancer sedimented at 3 S in the absence of protease inhibitors and at 4.5 S in the presence of 1 mM phenylmethylsulphonyl fluoride (PMSF) both in low and in high ionic strength.	Phenylmethylsulfonyl Fluoride	173-203	estrogen receptor 1	Homo sapiens	56-58
6271226-5	1981	Incubation of cells in the presence of 2-deoxyglucose, sodium azide and phenylmethylsulfonyl fluoride resulted in partial inhibition of progesterone secretion in response to hCG or sera.	Phenylmethylsulfonyl Fluoride	72-101	hypertrichosis 2 (generalised, congenital)	Homo sapiens	174-177
7314260-10	1981	Sucrose gradient centrifugation showed that cytoplasmic ER of colorectal cancer sedimented at 3 S in the absence of protease inhibitors and at 4.5 S in the presence of 1 mM phenylmethylsulphonyl fluoride (PMSF) both in low and in high ionic strength.	Phenylmethylsulfonyl Fluoride	205-209	estrogen receptor 1	Homo sapiens	56-58
6793069-2	1981	The plasma membrane-associated proteases were completely inhibited by the serine protease inhibitors, diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride and p-nitrophenyl-p-guanidinobenzoate, partially inhibited by soybean trypsin inhibitor and antipain, but were only weakly inhibited by L-1-tosylamino-2-phenylethyl chloromethyl ketone.	Phenylmethylsulfonyl Fluoride	131-160	subtilisin-like protease C1	Glycine max	74-89
6268171-4	1981	Tonin is inhibited by diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride at high concentrations (greater than 10(-2) M) and by soybean trypsin inhibitor and aprotinin.	Phenylmethylsulfonyl Fluoride	54-83	kallikrein 1-related peptidase C2	Rattus norvegicus	0-5
6988010-1	1980	Puff adder venom, which has been pretreated with phenylmethylsulphonyl fluoride and extensively dialysed, is capable of destroying selectively proteinase inhibitory activity in human plasma by an action of an EDTA-sensitive venom proteinase.	Phenylmethylsulfonyl Fluoride	49-79	endogenous retrovirus group K member 10	Homo sapiens	143-153
6452170-8	1981	Since both EDTA and phenylmethanesulphonyl fluoride could inhibit the activation of inactive renin by this metalloproteinase, it is suggested that the enzyme activates serine proteinase(s), which then activate inactive renin.	Phenylmethylsulfonyl Fluoride	20-51	renin	Homo sapiens	93-98
6452170-8	1981	Since both EDTA and phenylmethanesulphonyl fluoride could inhibit the activation of inactive renin by this metalloproteinase, it is suggested that the enzyme activates serine proteinase(s), which then activate inactive renin.	Phenylmethylsulfonyl Fluoride	20-51	renin	Homo sapiens	219-224
7002217-10	1980	Although previously unrecognized as a serine protease, dipeptidyl peptidase II (of pulp and pituitary origin) was strongly inhibited by (1 mM) diisopropyl phosphorofluoridate, p-nitrophenyl-p"-guanidinobenzoate, and phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	216-245	dipeptidyl peptidase 7	Bos taurus	55-78
6988010-3	1980	of such venom with human plasma at 25 degrees C leads to a concomitant increase in renin to 4.4 times control by 5 h. The activation of inactive renin was abolished by 10 mM EDTA and the rate of activation was reduced by 50% in the presence of 5 mM phenylmethylsulphonyl fluoride and by 90% when 0.32 mg/ml soybean trypsin inhibitor and 5 mM N-ethylmaleimide were added as well.	Phenylmethylsulfonyl Fluoride	249-279	renin	Homo sapiens	83-88
6988010-3	1980	of such venom with human plasma at 25 degrees C leads to a concomitant increase in renin to 4.4 times control by 5 h. The activation of inactive renin was abolished by 10 mM EDTA and the rate of activation was reduced by 50% in the presence of 5 mM phenylmethylsulphonyl fluoride and by 90% when 0.32 mg/ml soybean trypsin inhibitor and 5 mM N-ethylmaleimide were added as well.	Phenylmethylsulfonyl Fluoride	249-279	renin	Homo sapiens	145-150
6988010-1	1980	Puff adder venom, which has been pretreated with phenylmethylsulphonyl fluoride and extensively dialysed, is capable of destroying selectively proteinase inhibitory activity in human plasma by an action of an EDTA-sensitive venom proteinase.	Phenylmethylsulfonyl Fluoride	49-79	endogenous retrovirus group K member 10	Homo sapiens	230-240
335493-4	1977	That LIF might act as an esterase and a protease was further strengthened by the ability of pralidoxime methansulfonate (2-PAM) to reestablish LIF activity of supernatants previously inactivated by PMSF.	Phenylmethylsulfonyl Fluoride	198-202	LIF interleukin 6 family cytokine	Homo sapiens	5-8
212822-5	1978	Thus, using 10(-3) M PMSF, 3",5"-cGMP was active at concentrations higher than 10(-5) to 10(-4) M, and 2",3"-cCMP at concentrations higher than 3 X 10(-4) to 10(-3) M. The more pronounced LIF-inhibitory effect obtained by increased concentrations of PMSF could be overcome by raising the levels of the nucleotides, indicating that the interactions between PMSF and the nucleotides with LIF were mutally exclusive.	Phenylmethylsulfonyl Fluoride	21-25	LIF interleukin 6 family cytokine	Homo sapiens	188-191
212822-5	1978	Thus, using 10(-3) M PMSF, 3",5"-cGMP was active at concentrations higher than 10(-5) to 10(-4) M, and 2",3"-cCMP at concentrations higher than 3 X 10(-4) to 10(-3) M. The more pronounced LIF-inhibitory effect obtained by increased concentrations of PMSF could be overcome by raising the levels of the nucleotides, indicating that the interactions between PMSF and the nucleotides with LIF were mutally exclusive.	Phenylmethylsulfonyl Fluoride	21-25	LIF interleukin 6 family cytokine	Homo sapiens	386-389
203999-2	1977	3",5"-cGMP protects LIF against inactivation by the esterase inhibitor phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	71-100	LIF interleukin 6 family cytokine	Homo sapiens	20-23
203999-4	1977	Also reported previously, the synthetic phosphodiester bis-p-nitrophenyl phosphate (BNPP) but not various phosphomonoesters preserve LIF activity in the presence of the serine esterase inhibitor phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	195-224	LIF interleukin 6 family cytokine	Homo sapiens	133-136
322257-3	1977	The activity of leukocyte migration inhibitory factor (LIF) obtained from Sephadex-G-100-chromatographed supernatants of concanavalin-A-stimulated human lymphocytes was suppressed by two synthetic serine esterase and serine protease inhibitors (di-isopropylfluorophosphate (DFP) and phenylmethylfulfonyl fluoride (PMSF)).	Phenylmethylsulfonyl Fluoride	314-318	LIF interleukin 6 family cytokine	Homo sapiens	16-53
610003-5	1977	Isolation of tissue microsomes with Tris buffer, EDTA and the protease inhibitor phenylmethylsulfonylfluoride (PMSF), conditions which preserve the receptor molecules optimally, yielded about 50% of the tissue toxin-sites, 5% of the protein, 4% of the ATPase and less than 2% of the acetylcholinesterase (AChE).	Phenylmethylsulfonyl Fluoride	111-115	dynein axonemal heavy chain 8	Homo sapiens	252-258
610003-5	1977	Isolation of tissue microsomes with Tris buffer, EDTA and the protease inhibitor phenylmethylsulfonylfluoride (PMSF), conditions which preserve the receptor molecules optimally, yielded about 50% of the tissue toxin-sites, 5% of the protein, 4% of the ATPase and less than 2% of the acetylcholinesterase (AChE).	Phenylmethylsulfonyl Fluoride	111-115	acetylcholinesterase (Cartwright blood group)	Homo sapiens	283-303
6153537-7	1980	Experimental evidence suggests that low-affinity platelet factor 4 is originally secreted by platelets and then converted to beta-thromboglobulin by a platelet-derived, heat-labile protease that is inhibited by phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	211-240	pro-platelet basic protein	Homo sapiens	125-145
94652-7	1979	The protease activity was inhibited by 5 X 10(-3) M p-chloromercuribenzoate and by phenylmethyl sulfonyl fluoride, TPCK, and soybean trypsin inhibitor, therefore the enzymatic activity probably depends on a serine residue and a sulfhydryl group.	Phenylmethylsulfonyl Fluoride	83-113	kunitz trypsin protease inhibitor	Glycine max	133-150
115624-5	1979	Of these, however, only the combination of 5 mmol/l ethylenediamine tetraacetate, 1.5 mmol/l 8-hydroxyquinoline, and 7.5 mmol/l phenylmethylsulfonyl fluoride also maximized the rate of angiotensin I formation at both pH 5.5 and 7.4.	Phenylmethylsulfonyl Fluoride	128-157	angiotensinogen	Homo sapiens	185-198
489254-4	1979	The electrophoretic studies of the CP samples prepared both with and without potent proteolytic inhibitor, PMSF, revealed that CP is a single-chain protein with molecular weight of 130 000.	Phenylmethylsulfonyl Fluoride	107-111	ceruloplasmin	Homo sapiens	35-37
489254-4	1979	The electrophoretic studies of the CP samples prepared both with and without potent proteolytic inhibitor, PMSF, revealed that CP is a single-chain protein with molecular weight of 130 000.	Phenylmethylsulfonyl Fluoride	107-111	ceruloplasmin	Homo sapiens	127-129
233586-4	1979	LIF and the supernatant factor responsible for the cGMP-generating effect were both rendered inactive by treatment with the serine esterase and protease inhibitor, phenylmethylsulfonyl fluoride, indicating that these factors are closely related, if not identical.	Phenylmethylsulfonyl Fluoride	164-193	LIF interleukin 6 family cytokine	Homo sapiens	0-3
224450-1	1979	The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation.	Phenylmethylsulfonyl Fluoride	182-211	LIF interleukin 6 family cytokine	Homo sapiens	22-59
224450-1	1979	The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation.	Phenylmethylsulfonyl Fluoride	182-211	LIF interleukin 6 family cytokine	Homo sapiens	61-64
224450-1	1979	The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation.	Phenylmethylsulfonyl Fluoride	182-211	LIF interleukin 6 family cytokine	Homo sapiens	280-283
224450-1	1979	The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation.	Phenylmethylsulfonyl Fluoride	213-217	LIF interleukin 6 family cytokine	Homo sapiens	22-59
224450-1	1979	The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation.	Phenylmethylsulfonyl Fluoride	213-217	LIF interleukin 6 family cytokine	Homo sapiens	61-64
224450-1	1979	The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation.	Phenylmethylsulfonyl Fluoride	292-296	LIF interleukin 6 family cytokine	Homo sapiens	22-59
224450-1	1979	The human lymphokine, leucocyte migration-inhibitory factor (LIF), appears to be a serine esterase and protease by virtue of its susceptibility to the irreversible enzyme inhibitor, phenylmethylsulfonyl fluoride (PMSF), and by the ability of arginine esters and amides to protect LIF against PMSF-induced inactivation.	Phenylmethylsulfonyl Fluoride	292-296	LIF interleukin 6 family cytokine	Homo sapiens	61-64
224450-3	1979	Thus, molecules satisfying the substrate specificities of this lymphokine should (1) protect LIF against inactivation by PMSF, (2) reduce LIF activity in vitro on polymorphonuclear leucocytes, and (3) reduce the esterolytic activity of purified LIF-rich supernatants.	Phenylmethylsulfonyl Fluoride	121-125	LIF interleukin 6 family cytokine	Homo sapiens	93-96
224450-3	1979	Thus, molecules satisfying the substrate specificities of this lymphokine should (1) protect LIF against inactivation by PMSF, (2) reduce LIF activity in vitro on polymorphonuclear leucocytes, and (3) reduce the esterolytic activity of purified LIF-rich supernatants.	Phenylmethylsulfonyl Fluoride	121-125	LIF interleukin 6 family cytokine	Homo sapiens	138-141
224450-3	1979	Thus, molecules satisfying the substrate specificities of this lymphokine should (1) protect LIF against inactivation by PMSF, (2) reduce LIF activity in vitro on polymorphonuclear leucocytes, and (3) reduce the esterolytic activity of purified LIF-rich supernatants.	Phenylmethylsulfonyl Fluoride	121-125	LIF interleukin 6 family cytokine	Homo sapiens	138-141
224450-5	1979	Guanosine 3",5"-cyclic monophosphoric acid, which is capable of protecting LIF against PMSF-induced inhibition, also inhibited the esterolytic activity of the purified LIF preparation.	Phenylmethylsulfonyl Fluoride	87-91	LIF interleukin 6 family cytokine	Homo sapiens	75-78
224450-5	1979	Guanosine 3",5"-cyclic monophosphoric acid, which is capable of protecting LIF against PMSF-induced inhibition, also inhibited the esterolytic activity of the purified LIF preparation.	Phenylmethylsulfonyl Fluoride	87-91	LIF interleukin 6 family cytokine	Homo sapiens	168-171
359578-5	1978	Electron micrographs of sperm undergoing an A23187-induced acrosome reaction in the presence of the acrosin inhibitors benzamidine, p-amino-benzamidine and phenylmethylsulphonyl fluoride show that the acrosome reaction proceeds normally but that dispersal of the acrosomal contents is inhibited.	Phenylmethylsulfonyl Fluoride	156-186	acrosin	Cavia porcellus	100-107
627544-5	1978	Thioesterase II can restore the capacity for fatty acid synthesis to fatty acid synthetase in which the thioesterase component (thioesterase I) has been inactivated with phenylmethanesulfonyl fluoride or removed by trypsinization.	Phenylmethylsulfonyl Fluoride	170-200	oleoyl-ACP hydrolase	Rattus norvegicus	0-15
833129-4	1977	The inactivation of elastase 2 employed as radioiodinated tracer with an active site-specific reagent (phenylmethanesulfonyl fluoride) was necessary to prevent its binding by serum alpha1-antitrypsin and alpha2-macroglobulin while maintaining its immunoreactivity.	Phenylmethylsulfonyl Fluoride	103-133	elastase, neutrophil expressed	Homo sapiens	20-30
833129-4	1977	The inactivation of elastase 2 employed as radioiodinated tracer with an active site-specific reagent (phenylmethanesulfonyl fluoride) was necessary to prevent its binding by serum alpha1-antitrypsin and alpha2-macroglobulin while maintaining its immunoreactivity.	Phenylmethylsulfonyl Fluoride	103-133	serpin family A member 1	Homo sapiens	181-199
833129-4	1977	The inactivation of elastase 2 employed as radioiodinated tracer with an active site-specific reagent (phenylmethanesulfonyl fluoride) was necessary to prevent its binding by serum alpha1-antitrypsin and alpha2-macroglobulin while maintaining its immunoreactivity.	Phenylmethylsulfonyl Fluoride	103-133	alpha-2-macroglobulin	Homo sapiens	204-224
322257-3	1977	The activity of leukocyte migration inhibitory factor (LIF) obtained from Sephadex-G-100-chromatographed supernatants of concanavalin-A-stimulated human lymphocytes was suppressed by two synthetic serine esterase and serine protease inhibitors (di-isopropylfluorophosphate (DFP) and phenylmethylfulfonyl fluoride (PMSF)).	Phenylmethylsulfonyl Fluoride	314-318	LIF interleukin 6 family cytokine	Homo sapiens	55-58
322258-3	1977	The activity of leukocyte migration inhibitory factor (LIF) obtained from Sephadex-G-100-chromatographed supernatants of concanavalin-A-stimulated human lymphocytes was suppressed by two synthetic serine esterase and serine protease inhibitors (di-isopropylfluorophosphate (DEP) and phenylmethylsulfonyl fluoride (PMSF)).	Phenylmethylsulfonyl Fluoride	283-312	LIF interleukin 6 family cytokine	Homo sapiens	16-53
322258-3	1977	The activity of leukocyte migration inhibitory factor (LIF) obtained from Sephadex-G-100-chromatographed supernatants of concanavalin-A-stimulated human lymphocytes was suppressed by two synthetic serine esterase and serine protease inhibitors (di-isopropylfluorophosphate (DEP) and phenylmethylsulfonyl fluoride (PMSF)).	Phenylmethylsulfonyl Fluoride	283-312	LIF interleukin 6 family cytokine	Homo sapiens	55-58
322258-3	1977	The activity of leukocyte migration inhibitory factor (LIF) obtained from Sephadex-G-100-chromatographed supernatants of concanavalin-A-stimulated human lymphocytes was suppressed by two synthetic serine esterase and serine protease inhibitors (di-isopropylfluorophosphate (DEP) and phenylmethylsulfonyl fluoride (PMSF)).	Phenylmethylsulfonyl Fluoride	314-318	LIF interleukin 6 family cytokine	Homo sapiens	16-53
322258-3	1977	The activity of leukocyte migration inhibitory factor (LIF) obtained from Sephadex-G-100-chromatographed supernatants of concanavalin-A-stimulated human lymphocytes was suppressed by two synthetic serine esterase and serine protease inhibitors (di-isopropylfluorophosphate (DEP) and phenylmethylsulfonyl fluoride (PMSF)).	Phenylmethylsulfonyl Fluoride	314-318	LIF interleukin 6 family cytokine	Homo sapiens	55-58
322260-4	1977	To clarify this further, a wide variety of simple ester were tested for the ability to protect LIF against inactivation by the serine esterase inhibitor phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	153-182	LIF interleukin 6 family cytokine	Homo sapiens	95-98
322260-4	1977	To clarify this further, a wide variety of simple ester were tested for the ability to protect LIF against inactivation by the serine esterase inhibitor phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	184-188	LIF interleukin 6 family cytokine	Homo sapiens	95-98
335493-3	1977	The serine-specific inhibitors phenylmethylsulfonyl fluoride (PMSF) and physostigmine (eserine) markedly reduced LIF activity, whereas the histidine-specific inhibitors N-tosyl-L-lysine chloromethyl ketone (TLCK) and L-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) were inactive.	Phenylmethylsulfonyl Fluoride	31-60	LIF interleukin 6 family cytokine	Homo sapiens	113-116
335493-3	1977	The serine-specific inhibitors phenylmethylsulfonyl fluoride (PMSF) and physostigmine (eserine) markedly reduced LIF activity, whereas the histidine-specific inhibitors N-tosyl-L-lysine chloromethyl ketone (TLCK) and L-tosylamide-2-phenylethyl chloromethyl ketone (TPCK) were inactive.	Phenylmethylsulfonyl Fluoride	62-66	LIF interleukin 6 family cytokine	Homo sapiens	113-116
610003-5	1977	Isolation of tissue microsomes with Tris buffer, EDTA and the protease inhibitor phenylmethylsulfonylfluoride (PMSF), conditions which preserve the receptor molecules optimally, yielded about 50% of the tissue toxin-sites, 5% of the protein, 4% of the ATPase and less than 2% of the acetylcholinesterase (AChE).	Phenylmethylsulfonyl Fluoride	111-115	acetylcholinesterase (Cartwright blood group)	Homo sapiens	305-309
610003-7	1977	Mitochondria were effectively removed from homogenates by "differential" centrifugation, and ATPase-rich vesicles could be largely removed by causing their agglutination with calcium ions, or by controlled proteolysis in the absence of PMSF.	Phenylmethylsulfonyl Fluoride	236-240	dynein axonemal heavy chain 8	Homo sapiens	93-99
335493-4	1977	That LIF might act as an esterase and a protease was further strengthened by the ability of pralidoxime methansulfonate (2-PAM) to reestablish LIF activity of supernatants previously inactivated by PMSF.	Phenylmethylsulfonyl Fluoride	198-202	peptidylglycine alpha-amidating monooxygenase	Homo sapiens	123-126
982189-2	1976	When normal and iodine-poor rat thyroglobulin were extracted in the presence of phenylmethanesulfonyl fluoride, a serine protease inhibitor, very few components migrating faster than the 12S half-molecule were found.	Phenylmethylsulfonyl Fluoride	80-110	thyroglobulin	Rattus norvegicus	32-45
11637-1	1976	Leucocyte migration inhibitory factor (LIF) obtained from human lymphocytes stimulated with concanavalin A was consistently and irreversibly blocked by the serine-esterase inhibitor phenyl-methyl sulphonylfuoride (PMSF).	Phenylmethylsulfonyl Fluoride	214-218	LIF interleukin 6 family cytokine	Homo sapiens	0-37
11637-1	1976	Leucocyte migration inhibitory factor (LIF) obtained from human lymphocytes stimulated with concanavalin A was consistently and irreversibly blocked by the serine-esterase inhibitor phenyl-methyl sulphonylfuoride (PMSF).	Phenylmethylsulfonyl Fluoride	214-218	LIF interleukin 6 family cytokine	Homo sapiens	39-42
1149258-14	1975	With a direct radioimmunoassay, angiotensin I was generated in plasma by 3 h incubation at 37 degrees C and pH 5.6 after addition of phenylmethanesulfonyl fluoride, 8-hydroxyquinoline and 2,3-dimercaptopropanol (dimercaprol).	Phenylmethylsulfonyl Fluoride	133-163	angiotensinogen	Homo sapiens	32-45
4454040-3	1974	The proteolytic site of thrombin was modified with phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	51-80	coagulation factor II, thrombin	Homo sapiens	24-32
16659165-4	1975	The use of phenylmethylsulphonyl fluoride in the extraction medium gave only a partial protection of the nitrate reductase from the old root samples.	Phenylmethylsulfonyl Fluoride	11-41	nitrate reductase [NADH] 1	Zea mays	105-122
4454040-3	1974	The proteolytic site of thrombin was modified with phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	82-86	coagulation factor II, thrombin	Homo sapiens	24-32
4306345-0	1969	The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotyrpsin and trypsin.	Phenylmethylsulfonyl Fluoride	14-43	acetylcholinesterase (Cartwright blood group)	Homo sapiens	53-73
32630529-0	2020	Prolyl Endopeptidase-Like Facilitates the alpha-Synuclein Aggregation Seeding, and This Effect Is Reverted by Serine Peptidase Inhibitor PMSF.	Phenylmethylsulfonyl Fluoride	137-141	prolyl endopeptidase	Homo sapiens	0-20
33998011-10	2021	Notably, the downregulated expression of the genes encoding NaV1.8 and NaV1.9 were re-regulated and increased to control level by 10 Hz PMF application.	Phenylmethylsulfonyl Fluoride	136-139	sodium voltage-gated channel alpha subunit 10	Rattus norvegicus	60-66
33595315-8	2021	Much lower PMF in the EGL compared to that in the GEL indicated that gas molecules could migrate from the GEL to the nanobubble basement.	Phenylmethylsulfonyl Fluoride	11-14	gastrin	Homo sapiens	69-72
33443641-4	2021	The optimal activity of bee venom PLA2 was attained at pH 8 and 45  C. Cu2+, Ni2+, Fe2+, Ca2+, and Co2+ exhibited a complete activating effect on it, while Zn2+, Mn2+, NaN3, PMSF, N-Methylmaleimide, and EDTA have inhibitory effect.	Phenylmethylsulfonyl Fluoride	174-178	phospholipase A2	Apis mellifera	34-38
33294402-5	2020	Activity was not influenced by ions or organic substances (Triton, Tween, SDS and DMSO), but was completely inhibited by PMSF, suggesting that it belongs to the serine protease family.	Phenylmethylsulfonyl Fluoride	121-125	coagulation factor II, thrombin	Homo sapiens	161-176
32630529-0	2020	Prolyl Endopeptidase-Like Facilitates the alpha-Synuclein Aggregation Seeding, and This Effect Is Reverted by Serine Peptidase Inhibitor PMSF.	Phenylmethylsulfonyl Fluoride	137-141	synuclein alpha	Homo sapiens	42-57
32630529-8	2020	Furthermore, despite this lack of hydrolytic cleavage, the serine peptidase active site inhibitor phenylmethylsulfonyl fluoride (PMSF) abolished the enhancement of the alpha-Syn aggregation by PREPL.	Phenylmethylsulfonyl Fluoride	98-127	synemin	Homo sapiens	174-177
32630529-8	2020	Furthermore, despite this lack of hydrolytic cleavage, the serine peptidase active site inhibitor phenylmethylsulfonyl fluoride (PMSF) abolished the enhancement of the alpha-Syn aggregation by PREPL.	Phenylmethylsulfonyl Fluoride	98-127	prolyl endopeptidase like	Homo sapiens	193-198
32630529-8	2020	Furthermore, despite this lack of hydrolytic cleavage, the serine peptidase active site inhibitor phenylmethylsulfonyl fluoride (PMSF) abolished the enhancement of the alpha-Syn aggregation by PREPL.	Phenylmethylsulfonyl Fluoride	129-133	synemin	Homo sapiens	174-177
32630529-8	2020	Furthermore, despite this lack of hydrolytic cleavage, the serine peptidase active site inhibitor phenylmethylsulfonyl fluoride (PMSF) abolished the enhancement of the alpha-Syn aggregation by PREPL.	Phenylmethylsulfonyl Fluoride	129-133	prolyl endopeptidase like	Homo sapiens	193-198
32382667-9	2019	The presence of EDTA, SDS and PMSF inhibited phosphorylation by GST-Cdc5, while DTT had no effect.	Phenylmethylsulfonyl Fluoride	30-34	polo kinase CDC5	Saccharomyces cerevisiae S288C	68-72
32219980-9	2020	Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP-2 and MMP-9 induced by type-I IFN.	Phenylmethylsulfonyl Fluoride	0-30	matrix metallopeptidase 2	Bos taurus	126-131
32219980-9	2020	Phenylmethanesulfonyl fluoride and leupeptin (both are serine proteinase inhibitors) significantly repressed the clearance of MMP-2 and MMP-9 induced by type-I IFN.	Phenylmethylsulfonyl Fluoride	0-30	matrix metallopeptidase 9	Bos taurus	136-141
31475720-6	2019	Lipolysis profiles of polymethoxyflavone (PMF) loaded emulsions suggested that certain heat treatments could reduce the accessibility of lipase towards oil droplets and release of PMFs during lipolysis by enhancing the coverage of granules at the oil-water interface.	Phenylmethylsulfonyl Fluoride	42-45	lipase	Zea mays	137-143
31226285-1	2019	Although the three-dimensional structures of mouse and Torpedo californica acetylcholinesterase are very similar, their responses to the covalent sulfonylating agents benzenesulfonyl fluoride and phenylmethylsulfonyl fluoride are qualitatively different.	Phenylmethylsulfonyl Fluoride	196-225	acetylcholinesterase	Mus musculus	75-95
31226285-5	2019	Molecular dynamics simulations revealed that the mouse enzyme is substantially more flexible than the Torpedo enzyme, suggesting that enhanced "breathing motions" of the mouse enzyme relative to the Torpedo enzyme may explain why phenylmethylsulfonyl fluoride can reach the active site in mouse acetylcholinesterase, but not in the Torpedo enzyme.	Phenylmethylsulfonyl Fluoride	230-259	acetylcholinesterase	Mus musculus	295-315
31226285-7	2019	Whereas benzenesulfonyl fluoride closely approaches the active-site serine in both mouse and Torpedo acetylcholinesterase in such simulations, phenylmethylsulfonyl fluoride is able to approach the active-site serine of mouse acetylcholinesterase, but remains trapped above the bottleneck in the Torpedo enzyme.	Phenylmethylsulfonyl Fluoride	143-172	acetylcholinesterase	Mus musculus	225-245
29078076-7	2018	In agreement, bilitranslocase inhibition with 100 muM phenylmethanesulfonyl fluoride for 20 min reduced significantly HT, but not GC, effect on the vascular function upon stress induction.	Phenylmethylsulfonyl Fluoride	54-84	ceruloplasmin	Rattus norvegicus	14-29
30676743-2	2019	This study investigated the interaction of recombinant CYP3A4 with a nonspecific inhibitor metyrapone, antifungal drug fluconazole, and protease inhibitor phenylmethanesulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	187-191	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	55-61
30676743-3	2019	Metyrapone and fluconazole are classic type II ligands that inhibit CYP3A4 with medium strength by ligating to the heme iron, whereas PMSF, lacking the heme-ligating moiety, acts as a weak type I ligand and inhibitor of CYP3A4.	Phenylmethylsulfonyl Fluoride	134-138	cytochrome P450 family 3 subfamily A member 4	Homo sapiens	220-226
30176330-8	2018	Four enzymatic components (CP1, CP2, CP3 and CP4) were discriminated in cholinesterase activity in the membrane fraction according to their sensitivity to irreversible inhibitors mipafox, paraoxon, PMSF and iso-OMPA.	Phenylmethylsulfonyl Fluoride	198-202	butyrylcholinesterase	Gallus gallus	72-86
29596989-8	2018	The rAp activity was significantly inhibited by PMSF, Zn2+ and Fe2+ and the rAp had a broad substrate specificity for natural proteins and synthetic peptide substrates, and preferred substrates at P1 position with large hydrophobic side-chain groups.	Phenylmethylsulfonyl Fluoride	48-52	LDL receptor related protein associated protein 1	Rattus norvegicus	4-7
29596989-8	2018	The rAp activity was significantly inhibited by PMSF, Zn2+ and Fe2+ and the rAp had a broad substrate specificity for natural proteins and synthetic peptide substrates, and preferred substrates at P1 position with large hydrophobic side-chain groups.	Phenylmethylsulfonyl Fluoride	48-52	LDL receptor related protein associated protein 1	Rattus norvegicus	76-79
29596989-8	2018	The rAp activity was significantly inhibited by PMSF, Zn2+ and Fe2+ and the rAp had a broad substrate specificity for natural proteins and synthetic peptide substrates, and preferred substrates at P1 position with large hydrophobic side-chain groups.	Phenylmethylsulfonyl Fluoride	48-52	LDL receptor related protein associated protein 1	Rattus norvegicus	8-9
29721645-13	2018	MAP, CVP, Pmsf and CO decreased in MID1 and MID2 groups.	Phenylmethylsulfonyl Fluoride	10-14	probable E3 ubiquitin-protein ligase MID2	Oryctolagus cuniculus	44-48
29378341-11	2018	DISCUSSION: Whereas the use of the common protocol indicates the localization of MFN2 predominantly in SSM, the inhibition of nagarse by PMSF increases the signal of MFN2 in IFM to that of in SSM, indicating an underestimation of MFN2 in IFM.	Phenylmethylsulfonyl Fluoride	137-141	mitofusin 2	Mus musculus	166-170
29378341-11	2018	DISCUSSION: Whereas the use of the common protocol indicates the localization of MFN2 predominantly in SSM, the inhibition of nagarse by PMSF increases the signal of MFN2 in IFM to that of in SSM, indicating an underestimation of MFN2 in IFM.	Phenylmethylsulfonyl Fluoride	137-141	mitofusin 2	Mus musculus	166-170
28629701-6	2017	RESULTS TREATMENT: with the PMF mixture elicited the suppression of melanogenesis, the degradation of tyrosinase in lysosomes and the mislocalization of tyrosinase associated with the acidification of intracellular organelles, including melanosomes.	Phenylmethylsulfonyl Fluoride	28-31	tyrosinase	Homo sapiens	102-112
28855298-2	2017	BDNF elicits pMF by binding to its high-affinity receptor, tropomyosin receptor kinase B (TrkB), on phrenic motor neurons, potentially activating multiple downstream signaling cascades.	Phenylmethylsulfonyl Fluoride	13-16	brain-derived neurotrophic factor	Rattus norvegicus	0-4
28855298-2	2017	BDNF elicits pMF by binding to its high-affinity receptor, tropomyosin receptor kinase B (TrkB), on phrenic motor neurons, potentially activating multiple downstream signaling cascades.	Phenylmethylsulfonyl Fluoride	13-16	neurotrophic receptor tyrosine kinase 2	Rattus norvegicus	59-88
28855298-2	2017	BDNF elicits pMF by binding to its high-affinity receptor, tropomyosin receptor kinase B (TrkB), on phrenic motor neurons, potentially activating multiple downstream signaling cascades.	Phenylmethylsulfonyl Fluoride	13-16	neurotrophic receptor tyrosine kinase 2	Rattus norvegicus	90-94
28629701-6	2017	RESULTS TREATMENT: with the PMF mixture elicited the suppression of melanogenesis, the degradation of tyrosinase in lysosomes and the mislocalization of tyrosinase associated with the acidification of intracellular organelles, including melanosomes.	Phenylmethylsulfonyl Fluoride	28-31	tyrosinase	Homo sapiens	153-163
28629701-7	2017	The neutralization of cell organelle pH by ammonium chloride restored melanogenesis and the correct localization of tyrosinase to melanosomes, which had been suppressed by the PMF mixture.	Phenylmethylsulfonyl Fluoride	176-179	tyrosinase	Homo sapiens	116-126
28629701-8	2017	CONCLUSION: These results suggest that the PMF mixture suppresses the localization of tyrosinase to melanosomes and consequently inhibits melanogenesis due to the acidification of cell organelles, including melanosomes.	Phenylmethylsulfonyl Fluoride	43-46	tyrosinase	Homo sapiens	86-96
26856443-5	2016	The enzyme activity was inhibited by phenylmethylsulfonyl fluoride, which indicates that it belongs to serine protease group.	Phenylmethylsulfonyl Fluoride	37-66	coagulation factor II, thrombin	Homo sapiens	103-118
27890013-15	2017	Docking studies suggested that THL and PMSF could be the potent inhibitors for Rv1288 protein.	Phenylmethylsulfonyl Fluoride	39-43	hypothetical protein	Mycobacterium tuberculosis H37Rv	79-85
27642126-7	2017	The enzyme activity was strongly inhibited by soybean trypsin inhibitor (SBTI) and aprotinin which indicated it to be a serine protease, while other inhibitors like N-alpha-tosyl-l-phenylalanine chloromethyl ketone (TPCK), phenyl methane sulfonyl fluoride (PMSF), pepstatin and metal chelator EDTA did not inhibit its activity.	Phenylmethylsulfonyl Fluoride	257-261	kunitz trypsin protease inhibitor	Glycine max	54-78
27642126-7	2017	The enzyme activity was strongly inhibited by soybean trypsin inhibitor (SBTI) and aprotinin which indicated it to be a serine protease, while other inhibitors like N-alpha-tosyl-l-phenylalanine chloromethyl ketone (TPCK), phenyl methane sulfonyl fluoride (PMSF), pepstatin and metal chelator EDTA did not inhibit its activity.	Phenylmethylsulfonyl Fluoride	257-261	subtilisin-like protease C1	Glycine max	120-135
26363113-9	2017	The fibrinolytic activities of Harobin mutants were completely inhibited by PMSF and SBTI, but not by EDTA, EGTA, DTT, indicating that Harobin is a serine protease.	Phenylmethylsulfonyl Fluoride	76-80	cell division cycle 34, ubiqiutin conjugating enzyme	Rattus norvegicus	148-163
27507601-3	2016	In this work, four enzymatic components (CS1, CS2, CS3 and CS4) of cholinesterase activity have been discriminated in soluble fraction, according to their sensitivity to irreversible inhibitors mipafox, paraoxon, PMSF and iso-OMPA and to reversible inhibitors ethopropazine and BW284C51.	Phenylmethylsulfonyl Fluoride	213-217	butyrylcholinesterase	Gallus gallus	67-81
27381374-7	2016	RESULTS: Relative expression of beta-catenin differed significantly among groups (P = .0002), it was significantly higher in patients with PMF and SMF than in the control group, but did not differ between patients with PMF and SMF.	Phenylmethylsulfonyl Fluoride	139-142	catenin beta 1	Homo sapiens	32-44
27539903-3	2016	We now report that a polymethoxyflavone (PMF) mixture, extracted from orange peels, suppresses the UVB-induced expression of MMP-1 that involves the inhibition of c-jun N-terminal kinase (JNK) activity.	Phenylmethylsulfonyl Fluoride	41-44	matrix metallopeptidase 1	Homo sapiens	125-130
27539903-3	2016	We now report that a polymethoxyflavone (PMF) mixture, extracted from orange peels, suppresses the UVB-induced expression of MMP-1 that involves the inhibition of c-jun N-terminal kinase (JNK) activity.	Phenylmethylsulfonyl Fluoride	41-44	mitogen-activated protein kinase 8	Homo sapiens	163-186
27539903-3	2016	We now report that a polymethoxyflavone (PMF) mixture, extracted from orange peels, suppresses the UVB-induced expression of MMP-1 that involves the inhibition of c-jun N-terminal kinase (JNK) activity.	Phenylmethylsulfonyl Fluoride	41-44	mitogen-activated protein kinase 8	Homo sapiens	188-191
26942486-4	2016	The enzyme was strongly inhibited by PMSF, moderately by soybean trypsin inhibitor, indicating that the enzyme was a serine protease.	Phenylmethylsulfonyl Fluoride	37-41	subtilisin-like protease C1	Glycine max	117-132
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	32-61	macrophage migration inhibitory factor (glycosylation-inhibiting factor)	Mus musculus	25-28
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	32-61	macrophage migration inhibitory factor (glycosylation-inhibiting factor)	Mus musculus	92-95
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	32-61	ceruloplasmin	Mus musculus	104-106
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	63-67	macrophage migration inhibitory factor (glycosylation-inhibiting factor)	Mus musculus	25-28
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	63-67	macrophage migration inhibitory factor (glycosylation-inhibiting factor)	Mus musculus	92-95
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	63-67	ceruloplasmin	Mus musculus	104-106
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	96-100	macrophage migration inhibitory factor (glycosylation-inhibiting factor)	Mus musculus	25-28
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	96-100	macrophage migration inhibitory factor (glycosylation-inhibiting factor)	Mus musculus	92-95
26091949-8	2015	Covalent modification of MIF by phenylmethylsulfonyl fluoride (PMSF) resulted in binding of MIF-PMSF to CP immobilized on CM5 chip, the dissociation constant being 4.2 muM.	Phenylmethylsulfonyl Fluoride	96-100	ceruloplasmin	Mus musculus	104-106
26304077-5	2015	MPL L391-V392ins12 mutation was detected in 19 (7.66%)of the 248 patients with MPN, including 1 (1.92%) of 52 patients with PV, 14 (9.66%) of 145 with ET, and 4 (7.84%) of 51 with PMF.	Phenylmethylsulfonyl Fluoride	180-183	MPL proto-oncogene, thrombopoietin receptor	Homo sapiens	0-3
26209056-11	2015	In a multiple-regression analysis, norepinephrine (beta = 2.67, P = 0.0004) and age (beta = -0.061, P = 0.022) were associated with 1-min Pmsf.	Phenylmethylsulfonyl Fluoride	138-142	potassium calcium-activated channel subfamily M regulatory beta subunit 2	Homo sapiens	51-59
26269554-6	2015	Furthermore, intrathecal injections of a selective EPAC activator (8-pCPT-2"-Me-cAMP) were sufficient to elicit pMF.	Phenylmethylsulfonyl Fluoride	112-115	Rap guanine nucleotide exchange factor 3	Homo sapiens	51-55
26013015-4	2015	Furthermore, in vitro assays using phenylmethylsulfonyl fluoride (PMSF) and soybean trypsin inhibitor (SBTI) at 0.01 and 0.1 mM showed percentages of enzymatic inhibition between 0 and 16% for PMSF and 67 to 76% for SBTI, whereas in vivo assays indicated that SBTI caused between 50 and 90% mortality in males and between 80 and 100% in females.	Phenylmethylsulfonyl Fluoride	193-197	kunitz trypsin protease inhibitor	Glycine max	84-101
25208722-5	2015	Proteolytic cleavage was completely blocked by phenylmethylsulfonyl fluoride, indicating that a serine protease is responsible for exosomal ADAM15 shedding.	Phenylmethylsulfonyl Fluoride	47-76	coagulation factor II, thrombin	Homo sapiens	96-111
25208722-5	2015	Proteolytic cleavage was completely blocked by phenylmethylsulfonyl fluoride, indicating that a serine protease is responsible for exosomal ADAM15 shedding.	Phenylmethylsulfonyl Fluoride	47-76	ADAM metallopeptidase domain 15	Homo sapiens	140-146
25217485-8	2014	Interestingly, it was demonstrated that the CLint value of probenecid acyl glucuronidation in HLHs was increased by 1.7-fold in the presence of phenylmethylsulfonyl fluoride, which potently inhibited ABHD10 activity.	Phenylmethylsulfonyl Fluoride	144-173	abhydrolase domain containing 10, depalmitoylase	Homo sapiens	200-206
23878370-8	2013	Similar to iPMF, TNFalpha-induced pMF required spinal aPKC activity, as intrathecal delivery of a zeta-pseudosubstrate inhibitory peptide (PKCzeta-PS) 35 min following intrathecal TNFalpha arrested TNFalpha-induced pMF (28 +- 8% baseline; P < 0.05).	Phenylmethylsulfonyl Fluoride	34-37	tumor necrosis factor	Homo sapiens	17-25
24071788-0	2014	Kinetic interactions of a neuropathy potentiator (phenylmethylsulfonyl fluoride) with the neuropathy target esterase and other membrane bound esterases.	Phenylmethylsulfonyl Fluoride	50-79	patatin like phospholipase domain containing 6	Gallus gallus	90-116
24675880-5	2014	This apoE cleavage was inhibited by PMSF and alpha1-antichymotrypsin, but not neuroserpin-1 or inhibitors of thrombin and cathepsin G, supporting its identity as a chymotrypsin like protease.	Phenylmethylsulfonyl Fluoride	36-40	apolipoprotein E	Homo sapiens	5-9
24101248-7	2014	The method was validated using three known thrombin inhibitors: 4-(2-aminoethyl) benzenesulfonyl fluoride (IC50: 0.01 mM), p-amidinophenyl methanesulfonyl fluoride (IC50: 0.18 mM) and PMSF (IC50: 0.23 mM).	Phenylmethylsulfonyl Fluoride	184-188	coagulation factor II, thrombin	Homo sapiens	43-51
24803743-7	2014	PMSF attenuated cell aggregation on cathepsin G-treated substrates, but the effect was weak in cells pretreated with high concentrations of cathepsin G.	Phenylmethylsulfonyl Fluoride	0-4	cathepsin G	Homo sapiens	36-47
24803743-7	2014	PMSF attenuated cell aggregation on cathepsin G-treated substrates, but the effect was weak in cells pretreated with high concentrations of cathepsin G.	Phenylmethylsulfonyl Fluoride	0-4	cathepsin G	Homo sapiens	140-151
24803743-9	2014	Moreover, cathepsin G, but not elastase, induced aggregation on poly-L-lysine substrates which are not decomposed by these enzymes, and the action of cathepsin G was nearly completely attenuated by PMSF.	Phenylmethylsulfonyl Fluoride	198-202	cathepsin G	Homo sapiens	10-21
24803743-9	2014	Moreover, cathepsin G, but not elastase, induced aggregation on poly-L-lysine substrates which are not decomposed by these enzymes, and the action of cathepsin G was nearly completely attenuated by PMSF.	Phenylmethylsulfonyl Fluoride	198-202	cathepsin G	Homo sapiens	150-161
23290539-12	2013	The present results suggest that PMSF suppresses naloxone-precipitated withdrawal jumping in morphine-dependent mice, presumably through the inhibition of activities of tPA and plasmin belonging to the serine proteases family, which subsequently activates PAR-1.	Phenylmethylsulfonyl Fluoride	33-37	plasminogen activator, tissue	Mus musculus	169-172
23290539-12	2013	The present results suggest that PMSF suppresses naloxone-precipitated withdrawal jumping in morphine-dependent mice, presumably through the inhibition of activities of tPA and plasmin belonging to the serine proteases family, which subsequently activates PAR-1.	Phenylmethylsulfonyl Fluoride	33-37	coagulation factor II (thrombin) receptor	Mus musculus	256-261
22143069-3	2012	Although enzyme activities of LAL reagent were completely lost after heating at temperatures above 60  C for 10 min, gelating activities of coagulogen were retained even over 80  C. Phenylmethanesulfonyl fluoride (PMSF; 1 mmol/mL), a strong non-specific serine-protease inhibitor, did not completely inactivate serine-protease activities of LAL.	Phenylmethylsulfonyl Fluoride	214-218	lipase A, lysosomal acid type	Homo sapiens	30-33
22579962-3	2012	Enzyme activity of AMP48 was strongly inhibited by phenylmethanesulfonyl fluoride and soybean trypsin inhibitor, indicating that the enzyme was a plant serine protease.	Phenylmethylsulfonyl Fluoride	51-81	subtilisin-like protease C1	Glycine max	152-167
24385746-8	2013	JAK-2 V617F mutation was present in 35 patients, of whom 29 had ET (43.9%, 29/66) and 6 had PMF (54.5%, 6/11).	Phenylmethylsulfonyl Fluoride	92-95	Janus kinase 2	Homo sapiens	0-5
22690733-7	2012	The PMSF-binding information of the solved conjugated crystal structure was used to obtain a KFase and NFK complex using molecular docking.	Phenylmethylsulfonyl Fluoride	4-8	Kynurenine formamidase	Drosophila melanogaster	93-98
22366639-8	2012	In the meantime, pretreatment with PMSF reduced calpain expression below basal and increased beclin-1 expression above basal in tibial nerve, whereas it simply returned calpain and beclin-1 expression to their basal levels in spinal cord.	Phenylmethylsulfonyl Fluoride	35-39	beclin 1	Gallus gallus	93-101
22366639-8	2012	In the meantime, pretreatment with PMSF reduced calpain expression below basal and increased beclin-1 expression above basal in tibial nerve, whereas it simply returned calpain and beclin-1 expression to their basal levels in spinal cord.	Phenylmethylsulfonyl Fluoride	35-39	beclin 1	Gallus gallus	181-189
22354540-8	2012	The corresponding second-order rate constants of inhibition (ki = 12.04 x 10-2 and 0.54 x 10-2 muM-1 min-1, respectively) and the chemical hydrolysis constant of PMSF (kh = 0.0919 min-1) were simultaneously estimated.	Phenylmethylsulfonyl Fluoride	162-166	MUM1 like 1	Gallus gallus	95-106
22294686-9	2012	The AcMPAG deglucuronidation by recombinant ABHD10, HLC, and HLH were potently inhibited by AgNO(3), CdCl(2), CuCl(2), PMSF, bis-p-nitrophenylphosphate, and DTNB.	Phenylmethylsulfonyl Fluoride	119-123	abhydrolase domain containing 10, depalmitoylase	Homo sapiens	44-50
22294686-10	2012	The CL(int) value of AcMPAG formation from MPA, which was catalyzed by human UGT2B7, in HLH was increased by 1.8-fold in the presence of PMSF.	Phenylmethylsulfonyl Fluoride	137-141	UDP glucuronosyltransferase family 2 member B7	Homo sapiens	77-83
22363560-4	2012	The formation of PMF(2alpha) was reduced by indomethacin (a non-selective COX inhibitor), NS-398 (a selective COX-2 inhibitor) and SC-560 (a selective COX-1 inhibitor).	Phenylmethylsulfonyl Fluoride	17-20	cytochrome c oxidase II, mitochondrial	Mus musculus	110-115
22153250-4	2012	In addition, activity of enzymes such as beta-amylase, acid phosphatase, polyphenol oxidase and catalase was enhanced while alpha-amylase, alkaline phosphatase, protease and nitrate reductase activities declined due to PMF exposure.	Phenylmethylsulfonyl Fluoride	219-222	beta-amylase	Glycine max	41-53
22153250-4	2012	In addition, activity of enzymes such as beta-amylase, acid phosphatase, polyphenol oxidase and catalase was enhanced while alpha-amylase, alkaline phosphatase, protease and nitrate reductase activities declined due to PMF exposure.	Phenylmethylsulfonyl Fluoride	219-222	1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic	Glycine max	124-137
22919124-5	2012	The cell aggregation-inducing activity of CG was inhibited by pretreatment of CG with the serine protease inhibitors chymostatin and phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	133-162	cathepsin G	Homo sapiens	42-44
22919124-5	2012	The cell aggregation-inducing activity of CG was inhibited by pretreatment of CG with the serine protease inhibitors chymostatin and phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	133-162	cathepsin G	Homo sapiens	78-80
22363560-4	2012	The formation of PMF(2alpha) was reduced by indomethacin (a non-selective COX inhibitor), NS-398 (a selective COX-2 inhibitor) and SC-560 (a selective COX-1 inhibitor).	Phenylmethylsulfonyl Fluoride	17-20	cytochrome c oxidase I, mitochondrial	Mus musculus	151-156
21599633-5	2011	Its susceptibility to the nonspecific serine hydrolase inhibitor, phenylmethylsulfonyl fluoride (PMSF) paved the way for its use as a prototypical compound to design and synthesize a series of putative high affinity specific inhibitors of PMPMEase.	Phenylmethylsulfonyl Fluoride	66-95	liver carboxylesterase	Sus scrofa	239-247
21750123-7	2011	In human neutrophils, the Epac1 transcript was present, and Epac1 protein could be detected by Western blot analysis if the cells had been treated with the serine protease inhibitor PMSF.	Phenylmethylsulfonyl Fluoride	182-186	Rap guanine nucleotide exchange factor 3	Homo sapiens	26-31
21750123-7	2011	In human neutrophils, the Epac1 transcript was present, and Epac1 protein could be detected by Western blot analysis if the cells had been treated with the serine protease inhibitor PMSF.	Phenylmethylsulfonyl Fluoride	182-186	Rap guanine nucleotide exchange factor 3	Homo sapiens	60-65
22357532-8	2011	The results by two-dimensional electrophoresis and MS showed that the Stathmin expression was downregulated 3.4 times and 2.8 times in TOCP group, respectively, as compared with the control and PMSF intervention groups.	Phenylmethylsulfonyl Fluoride	194-198	stathmin 1	Gallus gallus	70-78
22001203-2	2011	Here, we report a remarkable discovery of protease inhibitor mediated reformation of peptide bonds by the serine protease inhibitor, PMSF in a diverse set of proteolyzed molecules.	Phenylmethylsulfonyl Fluoride	133-137	coagulation factor II, thrombin	Homo sapiens	106-121
21599633-5	2011	Its susceptibility to the nonspecific serine hydrolase inhibitor, phenylmethylsulfonyl fluoride (PMSF) paved the way for its use as a prototypical compound to design and synthesize a series of putative high affinity specific inhibitors of PMPMEase.	Phenylmethylsulfonyl Fluoride	97-101	liver carboxylesterase	Sus scrofa	239-247
20929049-9	2010	Pretreatment with protease inhibitor PMSF has been shown to protect the aging of NTE and prevent the development of delayed symptoms in hens.	Phenylmethylsulfonyl Fluoride	37-41	patatin like phospholipase domain containing 6	Gallus gallus	81-84
21330347-5	2011	The use of protease inhibitors indicated the involvement of a PMSF- and leupeptin-susceptible serine protease in ARG1 processing and activation.	Phenylmethylsulfonyl Fluoride	62-66	arginase 1	Homo sapiens	113-117
22146730-6	2011	Enzymatic activity of rDPP-IV was significantly reduced by 80 and 60% in the presence of sitagliptin and phenylmethylsulfonyl fluoride respectively.	Phenylmethylsulfonyl Fluoride	105-134	dipeptidylpeptidase 4	Rattus norvegicus	22-29
21069403-9	2011	The enzymatic activity was inhibited by 1 mM phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), indicating that it was a serine protease.	Phenylmethylsulfonyl Fluoride	45-74	coagulation factor II, thrombin	Homo sapiens	176-191
21069403-9	2011	The enzymatic activity was inhibited by 1 mM phenylmethyl sulfonylfluoride (PMSF) and hydrochloride 4-(2-aminoethyl)-benzenesulfonyl fluoride (AEBSF), indicating that it was a serine protease.	Phenylmethylsulfonyl Fluoride	76-80	coagulation factor II, thrombin	Homo sapiens	176-191
20614828-8	2010	PMSF showed a pure competitive type of inhibition with the serine protease enzyme.	Phenylmethylsulfonyl Fluoride	0-4	coagulation factor II, thrombin	Homo sapiens	59-74
20462810-13	2010	Characterization of stability in purchased/aged blood indicated that ethanolamides are subject to degradation mediated by intracellular membrane-bound FAAH, which has been shown to be inhibited by phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	197-226	fatty acid amide hydrolase	Homo sapiens	151-155
20462810-13	2010	Characterization of stability in purchased/aged blood indicated that ethanolamides are subject to degradation mediated by intracellular membrane-bound FAAH, which has been shown to be inhibited by phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	228-232	fatty acid amide hydrolase	Homo sapiens	151-155
20559002-8	2010	In addition, the decrease in CD44 expression can be abolished by pre-treating Pen ch 13 with a serine protease inhibitor, phenylmethyl-sulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	122-152	CD44 molecule (Indian blood group)	Homo sapiens	29-33
20559002-8	2010	In addition, the decrease in CD44 expression can be abolished by pre-treating Pen ch 13 with a serine protease inhibitor, phenylmethyl-sulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	122-152	proprotein convertase subtilisin/kexin type 1 inhibitor	Homo sapiens	78-81
19285387-7	2009	EDTA and PMSF at 10mM concentration strongly inhibited the lipase activity.	Phenylmethylsulfonyl Fluoride	9-13	DM80_RS12985	Burkholderia multivorans	59-65
19620706-3	2009	The activity of neuropathy target esterase is significantly inhibited by phenylmethylsulfonyl fluoride and paraoxon plus mipafox but not by paraoxon alone.	Phenylmethylsulfonyl Fluoride	73-102	patatin-like phospholipase domain containing 6	Mus musculus	16-42
19620706-7	2009	It is concluded that inhibition of neuropathy target esterase in mouse nervous tissues is not enough to disrupt the homeostasis of phosphatidylcholine and lysophosphatidylcholine and that the upregulation by phenylmethylsulfonyl fluoride may be the consequence of combined inhibition of neuropathy target esterase and other phospholipases.	Phenylmethylsulfonyl Fluoride	208-237	patatin-like phospholipase domain containing 6	Mus musculus	287-313
19110343-3	2009	Compounds 9 and 18 showed the most selective effects against human neutrophil elastase release, with IC(50) values of 0.8+/-0.1 and 1.7+/-0.6muM, respectively, and were 130-fold more potent than phenylmethylsulfonyl fluoride (PMSF), the positive control, in this anti-inflammatory assay.	Phenylmethylsulfonyl Fluoride	195-224	elastase, neutrophil expressed	Homo sapiens	67-86
19349639-7	2009	The results showed that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB, and PLB were significantly inhibited in both TOCP- and PMSF-treated mice, and the inhibition of NTE and NTE-LysoPLA or NTE-PLB showed a high correlation coefficient.	Phenylmethylsulfonyl Fluoride	133-137	patatin-like phospholipase domain containing 6	Mus musculus	42-45
19349639-7	2009	The results showed that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB, and PLB were significantly inhibited in both TOCP- and PMSF-treated mice, and the inhibition of NTE and NTE-LysoPLA or NTE-PLB showed a high correlation coefficient.	Phenylmethylsulfonyl Fluoride	133-137	patatin-like phospholipase domain containing 6	Mus musculus	47-50
19349639-7	2009	The results showed that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB, and PLB were significantly inhibited in both TOCP- and PMSF-treated mice, and the inhibition of NTE and NTE-LysoPLA or NTE-PLB showed a high correlation coefficient.	Phenylmethylsulfonyl Fluoride	133-137	patatin-like phospholipase domain containing 6	Mus musculus	47-50
19349639-7	2009	The results showed that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB, and PLB were significantly inhibited in both TOCP- and PMSF-treated mice, and the inhibition of NTE and NTE-LysoPLA or NTE-PLB showed a high correlation coefficient.	Phenylmethylsulfonyl Fluoride	133-137	patatin-like phospholipase domain containing 6	Mus musculus	47-50
19349639-7	2009	The results showed that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB, and PLB were significantly inhibited in both TOCP- and PMSF-treated mice, and the inhibition of NTE and NTE-LysoPLA or NTE-PLB showed a high correlation coefficient.	Phenylmethylsulfonyl Fluoride	133-137	patatin-like phospholipase domain containing 6	Mus musculus	47-50
19349639-7	2009	The results showed that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB, and PLB were significantly inhibited in both TOCP- and PMSF-treated mice, and the inhibition of NTE and NTE-LysoPLA or NTE-PLB showed a high correlation coefficient.	Phenylmethylsulfonyl Fluoride	133-137	patatin-like phospholipase domain containing 6	Mus musculus	47-50
19349639-8	2009	The NTE inhibited by TOCP was of the aged type, while nearly all NTE inhibited by PMSF was of the unaged type.	Phenylmethylsulfonyl Fluoride	82-86	patatin-like phospholipase domain containing 6	Mus musculus	65-68
19110343-3	2009	Compounds 9 and 18 showed the most selective effects against human neutrophil elastase release, with IC(50) values of 0.8+/-0.1 and 1.7+/-0.6muM, respectively, and were 130-fold more potent than phenylmethylsulfonyl fluoride (PMSF), the positive control, in this anti-inflammatory assay.	Phenylmethylsulfonyl Fluoride	226-230	elastase, neutrophil expressed	Homo sapiens	67-86
19160416-9	2009	Pretreatment of cells with protease inhibitors such as phenylmethyl sulfonyl fluoride (PMSF) partially blocked the heat-induced loss of eIF5A and prevented heat-induced cell death.	Phenylmethylsulfonyl Fluoride	55-85	eukaryotic translation initiation factor 5A	Homo sapiens	136-141
19160416-9	2009	Pretreatment of cells with protease inhibitors such as phenylmethyl sulfonyl fluoride (PMSF) partially blocked the heat-induced loss of eIF5A and prevented heat-induced cell death.	Phenylmethylsulfonyl Fluoride	87-91	eukaryotic translation initiation factor 5A	Homo sapiens	136-141
19090677-9	2009	Crystallographic analysis reveals the formation of a stable, novel covalent bond for PMSF between the catalytic nitrogen of the N-terminal proline and the sulfur of PMSF with complete, well-defined electron density in all three active sites of the MIF homotrimer.	Phenylmethylsulfonyl Fluoride	165-169	macrophage migration inhibitory factor	Homo sapiens	248-251
19396243-8	2009	The activity of the enzyme was totally lost in the presence of phenylmethylsulfonyl fluoride, which suggests that the purified enzyme is a serine protease.	Phenylmethylsulfonyl Fluoride	63-92	HtrA serine peptidase 2	Gallus gallus	139-154
19268997-4	2009	Inhibition of enzyme activity by serine protease inhibitors such as PMSF and chymostatin indicated that the enzyme belongs to the chymotrypsin-like serine protease class.	Phenylmethylsulfonyl Fluoride	68-72	coagulation factor II, thrombin	Homo sapiens	33-48
19268997-4	2009	Inhibition of enzyme activity by serine protease inhibitors such as PMSF and chymostatin indicated that the enzyme belongs to the chymotrypsin-like serine protease class.	Phenylmethylsulfonyl Fluoride	68-72	coagulation factor II, thrombin	Homo sapiens	148-163
19090677-8	2009	We also find that the commonly used protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), forms a covalent complex with MIF and inhibits the tautomerase activity.	Phenylmethylsulfonyl Fluoride	56-85	macrophage migration inhibitory factor	Homo sapiens	124-127
18361921-7	2008	Moreover, we observed that full-length mCLEC-2 could be cleaved probably by proteases sensitive to aprotinin and PMSF into a soluble form that partially existed as a disulfide-linked homodimer.	Phenylmethylsulfonyl Fluoride	113-117	C-type lectin domain family 1, member b	Mus musculus	39-46
19090677-8	2009	We also find that the commonly used protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), forms a covalent complex with MIF and inhibits the tautomerase activity.	Phenylmethylsulfonyl Fluoride	87-91	macrophage migration inhibitory factor	Homo sapiens	124-127
19090677-9	2009	Crystallographic analysis reveals the formation of a stable, novel covalent bond for PMSF between the catalytic nitrogen of the N-terminal proline and the sulfur of PMSF with complete, well-defined electron density in all three active sites of the MIF homotrimer.	Phenylmethylsulfonyl Fluoride	85-89	macrophage migration inhibitory factor	Homo sapiens	248-251
19479636-2	2009	Serine protease inhibitors such as pefabloc, 3,4 dichloroisocoumarin, phenyl methyl sulfonyl fluoride (PMSF) and metalloprotease inhibitors such as ethylene diamine tetracetic acid (EDTA) and 1,10 phenanthroline inhibited 65-92% serine protease activity in vitro.	Phenylmethylsulfonyl Fluoride	70-101	coagulation factor II, thrombin	Homo sapiens	0-15
19479636-2	2009	Serine protease inhibitors such as pefabloc, 3,4 dichloroisocoumarin, phenyl methyl sulfonyl fluoride (PMSF) and metalloprotease inhibitors such as ethylene diamine tetracetic acid (EDTA) and 1,10 phenanthroline inhibited 65-92% serine protease activity in vitro.	Phenylmethylsulfonyl Fluoride	103-107	coagulation factor II, thrombin	Homo sapiens	0-15
18755237-4	2008	The results demonstrated that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB and PLB were significantly inhibited in both TOCP- and PMSF-treated hens.	Phenylmethylsulfonyl Fluoride	138-142	patatin like phospholipase domain containing 6	Gallus gallus	48-51
18755237-4	2008	The results demonstrated that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB and PLB were significantly inhibited in both TOCP- and PMSF-treated hens.	Phenylmethylsulfonyl Fluoride	138-142	patatin like phospholipase domain containing 6	Gallus gallus	53-56
18755237-4	2008	The results demonstrated that the activities of NTE, NTE-LysoPLA, LysoPLA, NTE-PLB and PLB were significantly inhibited in both TOCP- and PMSF-treated hens.	Phenylmethylsulfonyl Fluoride	138-142	patatin like phospholipase domain containing 6	Gallus gallus	53-56
18755237-6	2008	Moreover, the NTE inhibited by TOCP was of the aged type, while nearly all of the NTE inhibited by PMSF was of the unaged type.	Phenylmethylsulfonyl Fluoride	99-103	patatin like phospholipase domain containing 6	Gallus gallus	82-85
18627771-10	2008	Activation of MMP-2 by MIP-133 was inhibited in the supernatants pretreated with the serine protease inhibitor, PMSF, and anti-MIP-133.	Phenylmethylsulfonyl Fluoride	112-116	matrix metallopeptidase 2	Homo sapiens	14-19
18656366-3	2008	In this study, we examined the effect of PMFs (PMF-1: 6,7,4",5"-tetramethoxy-5-monohydroxyflavone, PMF-2: 5,6,8,3",6"-pentamethoxy flavone, PMF-3: 5,6,7,3",4",5"-hexamethoxy flavone) on the degranulation in RBL-2H3 cells.	Phenylmethylsulfonyl Fluoride	41-44	polyamine-modulated factor 1	Rattus norvegicus	47-52
18656366-5	2008	Interestingly, PMF-combination (PMF-1+PMF-2; PMF-1+PMF-3) treatment enhanced the inhibition of degranulation compared with PMF-single treatment.	Phenylmethylsulfonyl Fluoride	15-18	polyamine-modulated factor 1	Rattus norvegicus	32-37
18656366-5	2008	Interestingly, PMF-combination (PMF-1+PMF-2; PMF-1+PMF-3) treatment enhanced the inhibition of degranulation compared with PMF-single treatment.	Phenylmethylsulfonyl Fluoride	15-18	polyamine-modulated factor 1	Rattus norvegicus	45-56
18376419-10	2008	Preincubating DRG neurons with the fatty acid amide hydrolase (FAAH) inhibitor phenylmethylsulphonyl fluoride (PMSF) attenuated the inhibitory actions of AEA on K(+) currents and Ca(2+) influx.	Phenylmethylsulfonyl Fluoride	79-109	fatty-acid amide hydrolase-like	Rattus norvegicus	35-61
18620539-4	2008	The neuroprotective effect of APC on the neurons was mediated by type 1 proteinase-activated receptor (PAR1), because the inactivation of the enzyme with phenylmethylsulfonyl fluoride or PAR1 blockade by a PAR1 peptide antagonist ((Tyr1)-TRAP-7) prevented the protective effect of APC.	Phenylmethylsulfonyl Fluoride	154-183	APC regulator of WNT signaling pathway	Homo sapiens	30-33
18620539-4	2008	The neuroprotective effect of APC on the neurons was mediated by type 1 proteinase-activated receptor (PAR1), because the inactivation of the enzyme with phenylmethylsulfonyl fluoride or PAR1 blockade by a PAR1 peptide antagonist ((Tyr1)-TRAP-7) prevented the protective effect of APC.	Phenylmethylsulfonyl Fluoride	154-183	coagulation factor II thrombin receptor	Homo sapiens	103-107
18376419-10	2008	Preincubating DRG neurons with the fatty acid amide hydrolase (FAAH) inhibitor phenylmethylsulphonyl fluoride (PMSF) attenuated the inhibitory actions of AEA on K(+) currents and Ca(2+) influx.	Phenylmethylsulfonyl Fluoride	79-109	fatty-acid amide hydrolase-like	Rattus norvegicus	63-67
18376419-10	2008	Preincubating DRG neurons with the fatty acid amide hydrolase (FAAH) inhibitor phenylmethylsulphonyl fluoride (PMSF) attenuated the inhibitory actions of AEA on K(+) currents and Ca(2+) influx.	Phenylmethylsulfonyl Fluoride	111-115	fatty-acid amide hydrolase-like	Rattus norvegicus	35-61
18376419-10	2008	Preincubating DRG neurons with the fatty acid amide hydrolase (FAAH) inhibitor phenylmethylsulphonyl fluoride (PMSF) attenuated the inhibitory actions of AEA on K(+) currents and Ca(2+) influx.	Phenylmethylsulfonyl Fluoride	111-115	fatty-acid amide hydrolase-like	Rattus norvegicus	63-67
17707670-4	2007	The enzyme was stable over a broad pH range (7 to 12), but was unstable at acidic pH, and at temperatures greater than 40 degrees C. The enzyme was inhibited by specific trypsin inhibitors: soybean trypsin inhibitor (SBTI), N-p-tosyl-l-lysine chloromethyl ketone (TLCK) and the serine protease inhibitor phenylmethyl sulfonylfluoride (PMSF).	Phenylmethylsulfonyl Fluoride	304-333	kunitz trypsin protease inhibitor	Glycine max	170-187
18227165-4	2008	In addition, the bactericidal activity of lactoferrin was inhibited by addition of phenylmethylsulfonyl fluoride, implicating the serine protease activity of lactoferrin in the killing of S. aureus.	Phenylmethylsulfonyl Fluoride	83-112	AT695_RS10460	Staphylococcus aureus	130-145
18095945-8	2008	Preincubation of cells with a serine protease inhibitor (PMSF), or a metalloprotease inhibitor (1,10-phenanthroline) prior to SM exposure partially prevented SM-induced degradation of laminin-5 subunits, beta3 and gamma2.	Phenylmethylsulfonyl Fluoride	57-61	eukaryotic translation elongation factor 1 beta 2 pseudogene 2	Homo sapiens	204-209
18095945-8	2008	Preincubation of cells with a serine protease inhibitor (PMSF), or a metalloprotease inhibitor (1,10-phenanthroline) prior to SM exposure partially prevented SM-induced degradation of laminin-5 subunits, beta3 and gamma2.	Phenylmethylsulfonyl Fluoride	57-61	tryptophanyl-tRNA synthetase 1	Homo sapiens	214-220
18781094-4	2008	Following the addition of phenylmethylsulfonyl fluoride, a serine protease inhibitor, to serum, the serum stability for ProGRP was similar to that in plasma.	Phenylmethylsulfonyl Fluoride	26-55	gastrin releasing peptide	Homo sapiens	120-126
17707670-4	2007	The enzyme was stable over a broad pH range (7 to 12), but was unstable at acidic pH, and at temperatures greater than 40 degrees C. The enzyme was inhibited by specific trypsin inhibitors: soybean trypsin inhibitor (SBTI), N-p-tosyl-l-lysine chloromethyl ketone (TLCK) and the serine protease inhibitor phenylmethyl sulfonylfluoride (PMSF).	Phenylmethylsulfonyl Fluoride	335-339	kunitz trypsin protease inhibitor	Glycine max	170-187
16123046-7	2005	The hydrolytic activity of BRS1 can be strongly inhibited by a serine protease inhibitor, phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	90-119	alpha/beta-Hydrolases superfamily protein	Arabidopsis thaliana	27-31
17404307-9	2007	Moreover, Pen c 13-mediated IL-8 release was significantly decreased in Ca(2+)-free medium and was abolished by the protease inhibitors, PMSF and 4-(2-aminoethyl) benzenesulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	137-141	proprotein convertase subtilisin/kexin type 1 inhibitor	Homo sapiens	10-13
17404307-9	2007	Moreover, Pen c 13-mediated IL-8 release was significantly decreased in Ca(2+)-free medium and was abolished by the protease inhibitors, PMSF and 4-(2-aminoethyl) benzenesulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	137-141	C-X-C motif chemokine ligand 8	Homo sapiens	28-32
17511349-2	2007	Presence of adenine in extracts, prepared with or without DTT/PMSF, caused a 45-60% decrease in XO and XO+XDH activities.	Phenylmethylsulfonyl Fluoride	62-66	xanthine dehydrogenase	Rattus norvegicus	106-109
17511349-3	2007	Removal of adenine by dialysis from extracts prepared with or without DTT/PMSF resulted in the recovery of XO and XO+XDH activities to almost their pre-dialysis control levels.	Phenylmethylsulfonyl Fluoride	74-78	xanthine dehydrogenase	Rattus norvegicus	117-120
17511349-4	2007	Enzyme activity after 24hr storage at -20 degrees C depended on the presence or absence of DTT/PMSF and adenine, with both XO and XO+XDH activities being lower in extracts with the combined presence of DTT/PMSF and adenine.	Phenylmethylsulfonyl Fluoride	206-210	xanthine dehydrogenase	Rattus norvegicus	133-136
17178871-6	2006	Under cell-free conditions in the presence of phenylmethylsulfonyl fluoride, AR underwent Ca(2+)-dependent degradation.	Phenylmethylsulfonyl Fluoride	46-75	androgen receptor	Homo sapiens	77-79
17170574-5	2006	The effect was remarkably reduced by adding phenylmethylsulfonylfluoride (PMSF), a serine proteinase inhibitor.	Phenylmethylsulfonyl Fluoride	44-72	endogenous retrovirus group K member 25	Homo sapiens	90-100
17170574-5	2006	The effect was remarkably reduced by adding phenylmethylsulfonylfluoride (PMSF), a serine proteinase inhibitor.	Phenylmethylsulfonyl Fluoride	74-78	endogenous retrovirus group K member 25	Homo sapiens	90-100
17075289-11	2006	Furthermore, phenylmethylsulfonyl fluoride, a protease inhibitor, inhibited the conversion from proMMP-9 to active MMP-9.	Phenylmethylsulfonyl Fluoride	13-42	matrix metallopeptidase 9	Homo sapiens	99-104
16441909-6	2006	Serine protease inhibitors such as aprotinin, soybean trypsin inhibitor and phenylmethanesulfonyl fluoride inhibited peptidase activity.	Phenylmethylsulfonyl Fluoride	76-106	subtilisin-like protease C1	Glycine max	0-15
16953388-6	2006	After brain stimulation reward thresholds stabilized, rats received intraperitoneal injections of the fatty acid amide hydrolase (FAAH) inhibitors phenylmethylsulfonyl fluoride (PMSF) (0, 15, 30, and 60 mg/kg) and URB-597 (0, 0.3, 1, and 3 mg/kg) and the selective anandamide reuptake inhibitor OMDM-2 (0, 3, 10, and 30 mg/kg).	Phenylmethylsulfonyl Fluoride	147-176	fatty-acid amide hydrolase-like	Rattus norvegicus	130-134
16497336-6	2006	PMF supplementation also reduced TG contents in the liver and heart and was able to regulate adipocytokines by significantly suppressing TNF-alpha, INF-gamma, IL-1beta and IL-6 expression and increasing adiponectin in IR hamsters.	Phenylmethylsulfonyl Fluoride	0-3	tumor necrosis factor	Homo sapiens	137-146
16497336-6	2006	PMF supplementation also reduced TG contents in the liver and heart and was able to regulate adipocytokines by significantly suppressing TNF-alpha, INF-gamma, IL-1beta and IL-6 expression and increasing adiponectin in IR hamsters.	Phenylmethylsulfonyl Fluoride	0-3	interleukin 1 beta	Homo sapiens	159-167
16497336-6	2006	PMF supplementation also reduced TG contents in the liver and heart and was able to regulate adipocytokines by significantly suppressing TNF-alpha, INF-gamma, IL-1beta and IL-6 expression and increasing adiponectin in IR hamsters.	Phenylmethylsulfonyl Fluoride	0-3	interleukin 6	Homo sapiens	172-176
16497336-6	2006	PMF supplementation also reduced TG contents in the liver and heart and was able to regulate adipocytokines by significantly suppressing TNF-alpha, INF-gamma, IL-1beta and IL-6 expression and increasing adiponectin in IR hamsters.	Phenylmethylsulfonyl Fluoride	0-3	adiponectin, C1Q and collagen domain containing	Homo sapiens	203-214
16458561-2	2006	The chinook salmon enzyme hydrolyzed the trypsin-specific synthetic substrate benzoyl-DL-arginine-p-nitroanilide (DL-BAPNA), and was inhibited by the general serine protease inhibitor phenyl methyl sulfonyl fluoride (PMSF), and also by the specific trypsin inhibitors - soybean trypsin inhibitor (SBTI) and benzamidine.	Phenylmethylsulfonyl Fluoride	184-215	kunitz trypsin protease inhibitor	Glycine max	249-266
16458561-2	2006	The chinook salmon enzyme hydrolyzed the trypsin-specific synthetic substrate benzoyl-DL-arginine-p-nitroanilide (DL-BAPNA), and was inhibited by the general serine protease inhibitor phenyl methyl sulfonyl fluoride (PMSF), and also by the specific trypsin inhibitors - soybean trypsin inhibitor (SBTI) and benzamidine.	Phenylmethylsulfonyl Fluoride	217-221	kunitz trypsin protease inhibitor	Glycine max	249-266
16423834-6	2006	By incubation with mK13, but not with mK1, mK9, or mK22, the 35-kDa pro-IL-1beta was cleaved into two major products with molecular masses of 17.5 and 22 kDa, and production was inhibited by phenylmethylsulfonyl fluoride, a serine protease inhibitor, but not by IL-1beta-converting enzyme inhibitors.	Phenylmethylsulfonyl Fluoride	191-220	keratin 13	Mus musculus	19-23
16423834-6	2006	By incubation with mK13, but not with mK1, mK9, or mK22, the 35-kDa pro-IL-1beta was cleaved into two major products with molecular masses of 17.5 and 22 kDa, and production was inhibited by phenylmethylsulfonyl fluoride, a serine protease inhibitor, but not by IL-1beta-converting enzyme inhibitors.	Phenylmethylsulfonyl Fluoride	191-220	keratin 1	Mus musculus	19-22
16423834-6	2006	By incubation with mK13, but not with mK1, mK9, or mK22, the 35-kDa pro-IL-1beta was cleaved into two major products with molecular masses of 17.5 and 22 kDa, and production was inhibited by phenylmethylsulfonyl fluoride, a serine protease inhibitor, but not by IL-1beta-converting enzyme inhibitors.	Phenylmethylsulfonyl Fluoride	191-220	interleukin 1 beta	Mus musculus	72-80
15967795-7	2005	Release of this peptide was blocked by pretreating cathepsin G with phenylmethylsulfonyl fluoride, strongly implying that oxidation introduced proteolytic cleavage sites into cathepsin G.	Phenylmethylsulfonyl Fluoride	68-97	cathepsin G	Homo sapiens	51-62
16100529-18	2005	PMSF treatment almost completely inhibited the FAAH activity in all three tissues, as did the highest dose of URB597 (3 mg kg(-1)) in spinal cord samples, whereas no obvious changes were seen ex vivo for the other treatments.	Phenylmethylsulfonyl Fluoride	0-4	fatty acid amide hydrolase	Mus musculus	47-51
15967795-7	2005	Release of this peptide was blocked by pretreating cathepsin G with phenylmethylsulfonyl fluoride, strongly implying that oxidation introduced proteolytic cleavage sites into cathepsin G.	Phenylmethylsulfonyl Fluoride	68-97	cathepsin G	Homo sapiens	175-186
16128395-4	2005	SpvB-mediated actin degradation occurred in the presence of the serine protease inhibitor phenylmethylsulfonylfluoride (PMSF), but was inhibited upon addition of novobiocin, an inhibitor of mono (ADP-ribosyl)transferase activity, or upon addition of EDTA.	Phenylmethylsulfonyl Fluoride	90-118	virulence protein	Salmonella enterica	0-4
16128395-4	2005	SpvB-mediated actin degradation occurred in the presence of the serine protease inhibitor phenylmethylsulfonylfluoride (PMSF), but was inhibited upon addition of novobiocin, an inhibitor of mono (ADP-ribosyl)transferase activity, or upon addition of EDTA.	Phenylmethylsulfonyl Fluoride	120-124	virulence protein	Salmonella enterica	0-4
15910884-4	2005	The enzyme was distinguishable from FAAH in terms of (1) the optimal activity at pH 5, (2) stimulation by dithiothreitol, (3) low sensitivity to two FAAH inhibitors (methyl arachidonyl fluorophosphonate and phenylmethylsulfonyl fluoride), and (4) high content in lung, spleen and macrophages of rat.	Phenylmethylsulfonyl Fluoride	207-236	fatty-acid amide hydrolase-like	Rattus norvegicus	36-40
16014037-8	2005	Incubation of kidney tissue with recombinant hemopexin resulted in loss of of glomerular ectoapyrase and sialoglycoproteins, as shown by immunohistochemistry, which effect can be inhibited with the serine protease inhibitor phenylmethanesulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	224-254	hemopexin	Rattus norvegicus	45-54
15907785-8	2005	The enzyme activity was detected at the basic range of pH and inhibited by serine protease inhibitors, diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride, but not by other protease inhibitors including a proteasome inhibitor, MG-132, and a tripeptidyl peptidase II (TPP II) inhibitor, AAF-CMK.	Phenylmethylsulfonyl Fluoride	135-164	coagulation factor II, thrombin	Homo sapiens	75-90
15907785-8	2005	The enzyme activity was detected at the basic range of pH and inhibited by serine protease inhibitors, diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride, but not by other protease inhibitors including a proteasome inhibitor, MG-132, and a tripeptidyl peptidase II (TPP II) inhibitor, AAF-CMK.	Phenylmethylsulfonyl Fluoride	135-164	tripeptidyl peptidase 2	Homo sapiens	251-275
15907785-8	2005	The enzyme activity was detected at the basic range of pH and inhibited by serine protease inhibitors, diisopropyl fluorophosphate and phenylmethylsulfonyl fluoride, but not by other protease inhibitors including a proteasome inhibitor, MG-132, and a tripeptidyl peptidase II (TPP II) inhibitor, AAF-CMK.	Phenylmethylsulfonyl Fluoride	135-164	tripeptidyl peptidase 2	Homo sapiens	277-283
12917417-9	2003	The proteolytic activity in SOCS1-/- BMMCs was selectively inhibited by phenylmethylsulfonyl fluoride and soybean trypsin inhibitor, suggesting that the protease regulated by SOCS1 is a tryptase.	Phenylmethylsulfonyl Fluoride	72-101	suppressor of cytokine signaling 1	Mus musculus	28-33
15607631-6	2005	This activity was completely blocked by 10 mM phenylmethylsulphonyl fluoride (PMSF), a serine proteinase inhibitor, suggesting that this extracellular proteinase belongs to the serine proteinase class.	Phenylmethylsulfonyl Fluoride	46-76	endogenous retrovirus group K member 7	Homo sapiens	94-104
15607631-6	2005	This activity was completely blocked by 10 mM phenylmethylsulphonyl fluoride (PMSF), a serine proteinase inhibitor, suggesting that this extracellular proteinase belongs to the serine proteinase class.	Phenylmethylsulfonyl Fluoride	46-76	endogenous retrovirus group K member 7	Homo sapiens	151-161
15607631-6	2005	This activity was completely blocked by 10 mM phenylmethylsulphonyl fluoride (PMSF), a serine proteinase inhibitor, suggesting that this extracellular proteinase belongs to the serine proteinase class.	Phenylmethylsulfonyl Fluoride	46-76	endogenous retrovirus group K member 7	Homo sapiens	151-161
15607631-6	2005	This activity was completely blocked by 10 mM phenylmethylsulphonyl fluoride (PMSF), a serine proteinase inhibitor, suggesting that this extracellular proteinase belongs to the serine proteinase class.	Phenylmethylsulfonyl Fluoride	78-82	endogenous retrovirus group K member 7	Homo sapiens	94-104
15607631-6	2005	This activity was completely blocked by 10 mM phenylmethylsulphonyl fluoride (PMSF), a serine proteinase inhibitor, suggesting that this extracellular proteinase belongs to the serine proteinase class.	Phenylmethylsulfonyl Fluoride	78-82	endogenous retrovirus group K member 7	Homo sapiens	151-161
15607631-6	2005	This activity was completely blocked by 10 mM phenylmethylsulphonyl fluoride (PMSF), a serine proteinase inhibitor, suggesting that this extracellular proteinase belongs to the serine proteinase class.	Phenylmethylsulfonyl Fluoride	78-82	endogenous retrovirus group K member 7	Homo sapiens	151-161
15897155-5	2005	Phenylmethylsulfonyl fluoride (PMSF) was included as a non-neuropathic inhibitor of NTE.	Phenylmethylsulfonyl Fluoride	0-29	patatin like phospholipase domain containing 6	Gallus gallus	84-87
15897155-5	2005	Phenylmethylsulfonyl fluoride (PMSF) was included as a non-neuropathic inhibitor of NTE.	Phenylmethylsulfonyl Fluoride	31-35	patatin like phospholipase domain containing 6	Gallus gallus	84-87
15564667-7	2004	The catalytic activity of the Est3 esterase was strongly inhibited by phenylmethylsulfonyl fluoride (PMSF) and diethyl p-nitrophenyl phosphate.	Phenylmethylsulfonyl Fluoride	70-99	PaaI family thioesterase	Saccharolobus solfataricus	35-43
15564667-7	2004	The catalytic activity of the Est3 esterase was strongly inhibited by phenylmethylsulfonyl fluoride (PMSF) and diethyl p-nitrophenyl phosphate.	Phenylmethylsulfonyl Fluoride	101-105	PaaI family thioesterase	Saccharolobus solfataricus	35-43
15494208-3	2005	Both fMLP and IL-8 increased CD38 in supernatants, which was inhibitable with PMSF.	Phenylmethylsulfonyl Fluoride	78-82	formyl peptide receptor 1	Homo sapiens	5-9
15494208-3	2005	Both fMLP and IL-8 increased CD38 in supernatants, which was inhibitable with PMSF.	Phenylmethylsulfonyl Fluoride	78-82	C-X-C motif chemokine ligand 8	Homo sapiens	14-18
15494208-3	2005	Both fMLP and IL-8 increased CD38 in supernatants, which was inhibitable with PMSF.	Phenylmethylsulfonyl Fluoride	78-82	CD38 molecule	Homo sapiens	29-33
15381092-6	2004	In the presence of PMSF, a serine proteinase inhibitor, the activities for these mutants were inhibited by 86.5% and 80.1%, respectively, indicating that the catalytic triad of the trypsin had been successfully introduced into porcine pepsin.	Phenylmethylsulfonyl Fluoride	19-23	endogenous retrovirus group K member 25	Homo sapiens	34-44
15145953-5	2004	The enzyme activity was strongly inhibited by phenylmethylsulfonyl fluoride, CuSO(4), and ONO-5046 (a specific inhibitor of neutrophil elastase), but not by aprotinin, leupeptin, benzamidine, and EDTA.	Phenylmethylsulfonyl Fluoride	46-75	elastase, neutrophil expressed	Homo sapiens	124-143
15223848-2	2004	Addition of phenyl-methyl-sulfonyl-fluoride (PMSF) to a final concentration of 20 microg/mL semen at 4 degrees C has preserved sperm concentrations and HspA2 isoform ratios, even at room temperature, simulating a shipping delay in moderate ambient temperatures.	Phenylmethylsulfonyl Fluoride	45-49	heat shock protein family A (Hsp70) member 2	Homo sapiens	152-157
15165836-7	2004	The fatty acid amide hydrolase (FAAH) inhibitor phenylmethylsulfonyl fluoride (PMSF) and the anandamide uptake inhibitor AM404 inhibited transmitter release, implying that the antagonist effects were mediated by blockade of endocannabinoid activity.	Phenylmethylsulfonyl Fluoride	48-77	fatty-acid amide hydrolase-like	Rattus norvegicus	32-36
15165836-7	2004	The fatty acid amide hydrolase (FAAH) inhibitor phenylmethylsulfonyl fluoride (PMSF) and the anandamide uptake inhibitor AM404 inhibited transmitter release, implying that the antagonist effects were mediated by blockade of endocannabinoid activity.	Phenylmethylsulfonyl Fluoride	79-83	fatty-acid amide hydrolase-like	Rattus norvegicus	32-36
14996700-4	2004	Recombinant PRCP is inhibited by leupeptin, angiotensin II, bradykinin, anti-PRCP, diisopropyl-fluorophosphonate (DFP), phenylmethylsulfonyl fluoride (PMSF), and Z-Pro-Proaldehyde-dimethyl acetate, but not by 1 mM EDTA (ethylenediaminetetraacetic acid), bradykinin 1-5, or angiotensin 1-7.	Phenylmethylsulfonyl Fluoride	120-149	prolylcarboxypeptidase	Homo sapiens	12-16
14996700-4	2004	Recombinant PRCP is inhibited by leupeptin, angiotensin II, bradykinin, anti-PRCP, diisopropyl-fluorophosphonate (DFP), phenylmethylsulfonyl fluoride (PMSF), and Z-Pro-Proaldehyde-dimethyl acetate, but not by 1 mM EDTA (ethylenediaminetetraacetic acid), bradykinin 1-5, or angiotensin 1-7.	Phenylmethylsulfonyl Fluoride	151-155	prolylcarboxypeptidase	Homo sapiens	12-16
15033978-4	2004	TTR-mediated proteolysis was inhibited by serine protease inhibitors (phenylmethanesulfonyl fluoride, Pefabloc, diisopropyl fluorophosphate, chymostatin, and N(alpha)-p-tosyl-l-phenylala-nine-chloromethyl ketone), suggesting a chymotrypsin-like activity.	Phenylmethylsulfonyl Fluoride	70-100	transthyretin	Homo sapiens	0-3
14704997-9	2004	Detection of cholesterol esterase (98 kDa) in crude PPL preparations depended on addition of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF) to the incubation mix, as demonstrated by spiking with cholesterol esterase.	Phenylmethylsulfonyl Fluoride	116-145	carboxyl ester lipase	Homo sapiens	13-33
14704997-9	2004	Detection of cholesterol esterase (98 kDa) in crude PPL preparations depended on addition of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF) to the incubation mix, as demonstrated by spiking with cholesterol esterase.	Phenylmethylsulfonyl Fluoride	116-145	carboxyl ester lipase	Homo sapiens	208-228
14704997-9	2004	Detection of cholesterol esterase (98 kDa) in crude PPL preparations depended on addition of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF) to the incubation mix, as demonstrated by spiking with cholesterol esterase.	Phenylmethylsulfonyl Fluoride	147-151	carboxyl ester lipase	Homo sapiens	13-33
14704997-9	2004	Detection of cholesterol esterase (98 kDa) in crude PPL preparations depended on addition of the protease inhibitor phenylmethylsulfonyl fluoride (PMSF) to the incubation mix, as demonstrated by spiking with cholesterol esterase.	Phenylmethylsulfonyl Fluoride	147-151	carboxyl ester lipase	Homo sapiens	208-228
12917417-9	2003	The proteolytic activity in SOCS1-/- BMMCs was selectively inhibited by phenylmethylsulfonyl fluoride and soybean trypsin inhibitor, suggesting that the protease regulated by SOCS1 is a tryptase.	Phenylmethylsulfonyl Fluoride	72-101	suppressor of cytokine signaling 1	Mus musculus	175-180
12917417-9	2003	The proteolytic activity in SOCS1-/- BMMCs was selectively inhibited by phenylmethylsulfonyl fluoride and soybean trypsin inhibitor, suggesting that the protease regulated by SOCS1 is a tryptase.	Phenylmethylsulfonyl Fluoride	72-101	tryptase alpha/beta 1	Mus musculus	186-194
12960243-7	2003	The serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF) augmented membrane expression of PR3 in unactivated and PMA-stimulated neutrophils.	Phenylmethylsulfonyl Fluoride	30-59	proteinase 3	Homo sapiens	100-103
12960243-7	2003	The serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF) augmented membrane expression of PR3 in unactivated and PMA-stimulated neutrophils.	Phenylmethylsulfonyl Fluoride	61-65	proteinase 3	Homo sapiens	100-103
12007650-6	2002	Protease A activity was inhibited by serine protease inhibitors, such as phenylmethylsulfonyl fluoride and soybean trypsin inhibitor; moreover, the activity could be blocked by treatment with 20 mM of 1,10-phenanthroline, but could not be restored by adding metal ions.	Phenylmethylsulfonyl Fluoride	73-102	subtilisin-like protease C1	Glycine max	37-52
12562891-11	2003	The fatty acid amide hydrolase (FAAH) inhibitors phenylmethylsulfonyl fluoride (PMSF) (20 microM) and OL53 (1 microM) did not enhance the effect of anandamide in either the slice or dissociated neuron preparations.	Phenylmethylsulfonyl Fluoride	49-78	fatty-acid amide hydrolase-like	Rattus norvegicus	32-36
12439931-12	2002	PMSF had an effect on inhibiting activity of serine protease, while EDTA had not.	Phenylmethylsulfonyl Fluoride	0-4	coagulation factor II, thrombin	Homo sapiens	45-60
12220886-10	2002	All the antibodies were inhibited by phenylmethylsulphonyl fluoride (PMSF), indicating the presence of a serine residue in their active sites and were inhibited by the cholinesterase active site inhibitors tetraisopropyl pyrophosphoramide (iso-OMPA) and pyridostigmine.	Phenylmethylsulfonyl Fluoride	37-67	butyrylcholinesterase	Mus musculus	168-182
12220886-10	2002	All the antibodies were inhibited by phenylmethylsulphonyl fluoride (PMSF), indicating the presence of a serine residue in their active sites and were inhibited by the cholinesterase active site inhibitors tetraisopropyl pyrophosphoramide (iso-OMPA) and pyridostigmine.	Phenylmethylsulfonyl Fluoride	69-73	butyrylcholinesterase	Mus musculus	168-182
12177188-6	2002	Conversely, the FAAH inhibitors phenylmethylsulfonyl fluoride and methyl-arachidonoyl fluorophosphonate were much more effective in 6-OHDA-lesioned animals.	Phenylmethylsulfonyl Fluoride	32-61	fatty-acid amide hydrolase-like	Rattus norvegicus	16-20
12529258-7	2003	This action seems to be dependent on esterasic activity because serine protease inhibitors like PMSF and aprotinin were able to block the vasorelaxant effect of PreR-Co.	Phenylmethylsulfonyl Fluoride	96-100	subtilisin-like protease C1	Glycine max	64-79
12576084-4	2003	The deamidase activity was inhibited by Ebelactone B and the serine protease inhibitor, phenylmethanesulfonyl fluoride (PMSF), while the degradation of the synthetic stereoisomer, Asn-Trp-Phe-NH(2) (N(l)WF-NH(2)), was sensitive to the divalent cation-chelating agent, o-phenanthroline, and aminopeptidase inhibitors, amastatin and bestatin.	Phenylmethylsulfonyl Fluoride	88-118	cathepsin A	Homo sapiens	4-13
12576084-4	2003	The deamidase activity was inhibited by Ebelactone B and the serine protease inhibitor, phenylmethanesulfonyl fluoride (PMSF), while the degradation of the synthetic stereoisomer, Asn-Trp-Phe-NH(2) (N(l)WF-NH(2)), was sensitive to the divalent cation-chelating agent, o-phenanthroline, and aminopeptidase inhibitors, amastatin and bestatin.	Phenylmethylsulfonyl Fluoride	120-124	cathepsin A	Homo sapiens	4-13
22905396-8	2002	The stimulation of the MMP and Ca(2+)-ATPase activities remain unaffected by the inhibitors of serine, thiol and cysteine groups of proteases such as phenylmethylsulfonylfluoride (PMSF), Bowman Birk inhibitor (BBI), chymostatin, N-ethylmaleimide, leupeptin, antipain and pepstatin.	Phenylmethylsulfonyl Fluoride	180-184	matrix metallopeptidase 2	Bos taurus	23-26
12423273-9	2002	Phenylmethylsulfonyl fluoride significantly inhibited shedding of sTNF-R and a synergistic effect of TNF and IFN-gamma on apoptosis was observed.	Phenylmethylsulfonyl Fluoride	0-29	tumor necrosis factor	Homo sapiens	67-70
12423273-9	2002	Phenylmethylsulfonyl fluoride significantly inhibited shedding of sTNF-R and a synergistic effect of TNF and IFN-gamma on apoptosis was observed.	Phenylmethylsulfonyl Fluoride	0-29	interferon gamma	Homo sapiens	109-118
12183060-5	2002	Phenylmethylsulfonyl fluoride (PMSF) was the only inhibitor able to prevent formation of mactinin by cell lysate degradation of alpha-actinin, suggesting that a serine protease is responsible for the digestion.	Phenylmethylsulfonyl Fluoride	0-29	actinin alpha 1	Homo sapiens	128-141
12183060-5	2002	Phenylmethylsulfonyl fluoride (PMSF) was the only inhibitor able to prevent formation of mactinin by cell lysate degradation of alpha-actinin, suggesting that a serine protease is responsible for the digestion.	Phenylmethylsulfonyl Fluoride	31-35	actinin alpha 1	Homo sapiens	128-141
12067299-6	2002	Treatment of PR3 with PMSF completely abolished CD14 degradation.	Phenylmethylsulfonyl Fluoride	22-26	proteinase 3	Homo sapiens	13-16
12067299-6	2002	Treatment of PR3 with PMSF completely abolished CD14 degradation.	Phenylmethylsulfonyl Fluoride	22-26	CD14 molecule	Homo sapiens	48-52
11950785-9	2002	Carboxylesterases with the same functional subsites had a similar profile on substrate specificity and sensitivity toward phenylmethylsulfonyl fluoride (PMSF) and paraoxon, suggesting that these subsites play determinant roles in the recognition of substrates and inhibitors.	Phenylmethylsulfonyl Fluoride	122-151	carboxylesterase 2	Homo sapiens	0-17
11950785-9	2002	Carboxylesterases with the same functional subsites had a similar profile on substrate specificity and sensitivity toward phenylmethylsulfonyl fluoride (PMSF) and paraoxon, suggesting that these subsites play determinant roles in the recognition of substrates and inhibitors.	Phenylmethylsulfonyl Fluoride	153-157	carboxylesterase 2	Homo sapiens	0-17
11754869-6	2002	The main objective of this investigation was to study the effect of DFP administration on NF subunit levels when OPIDN is prevented or potentiated by pretreatment or post-treatment with phenylmethylsulfonyl fluoride (PMSF), respectively.	Phenylmethylsulfonyl Fluoride	186-215	CCAAT/enhancer binding protein beta	Gallus gallus	90-92
22896886-1	2002	Wheat germ lipase (WGL) was inactivated by chemical modification of histidine, serine and carboxyl groups of Asp/Glu residues with diethyl pyrocarbonate (DEPC), phenyl methyl sulfonyl fluoride (PMSF) and 1-ethyl-3-(3-dimethylaminopropyl) carbodi-imide (EDC), respectively.	Phenylmethylsulfonyl Fluoride	161-192	probable feruloyl esterase A	Triticum aestivum	11-17
22896886-1	2002	Wheat germ lipase (WGL) was inactivated by chemical modification of histidine, serine and carboxyl groups of Asp/Glu residues with diethyl pyrocarbonate (DEPC), phenyl methyl sulfonyl fluoride (PMSF) and 1-ethyl-3-(3-dimethylaminopropyl) carbodi-imide (EDC), respectively.	Phenylmethylsulfonyl Fluoride	194-198	probable feruloyl esterase A	Triticum aestivum	11-17
11796822-13	2002	When recombinant rabbit TNFalpha was incubated with synovial fluids obtained at 2 hours after IL-8 injection, the resultant TNFalpha activity was significantly decreased, an event that was completely restored by a serine protease inhibitor, phenylmethylsulphonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	241-271	tumor necrosis factor	Oryctolagus cuniculus	24-32
11849485-6	2002	Inhibition profile exhibited by PMSF suggested the B. pumilus protease to be an alkaline serine protease.	Phenylmethylsulfonyl Fluoride	32-36	AKO65_RS04470	Bacillus pumilus	89-104
11796822-13	2002	When recombinant rabbit TNFalpha was incubated with synovial fluids obtained at 2 hours after IL-8 injection, the resultant TNFalpha activity was significantly decreased, an event that was completely restored by a serine protease inhibitor, phenylmethylsulphonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	241-271	interleukin-8	Oryctolagus cuniculus	94-98
11796822-13	2002	When recombinant rabbit TNFalpha was incubated with synovial fluids obtained at 2 hours after IL-8 injection, the resultant TNFalpha activity was significantly decreased, an event that was completely restored by a serine protease inhibitor, phenylmethylsulphonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	241-271	tumor necrosis factor	Oryctolagus cuniculus	124-132
11796822-13	2002	When recombinant rabbit TNFalpha was incubated with synovial fluids obtained at 2 hours after IL-8 injection, the resultant TNFalpha activity was significantly decreased, an event that was completely restored by a serine protease inhibitor, phenylmethylsulphonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	273-277	tumor necrosis factor	Oryctolagus cuniculus	24-32
11796822-13	2002	When recombinant rabbit TNFalpha was incubated with synovial fluids obtained at 2 hours after IL-8 injection, the resultant TNFalpha activity was significantly decreased, an event that was completely restored by a serine protease inhibitor, phenylmethylsulphonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	273-277	tumor necrosis factor	Oryctolagus cuniculus	124-132
11796822-14	2002	Furthermore, TNFalpha activity was unveiled in the joints when IL-8 was intra-articularly injected with PMSF.	Phenylmethylsulfonyl Fluoride	104-108	tumor necrosis factor	Oryctolagus cuniculus	13-21
11796822-14	2002	Furthermore, TNFalpha activity was unveiled in the joints when IL-8 was intra-articularly injected with PMSF.	Phenylmethylsulfonyl Fluoride	104-108	interleukin-8	Oryctolagus cuniculus	63-67
11722648-5	2001	Enzymatic activity of RMCP-I was required to enhance IgE and IgG1, because two inhibitors for chymotryptic enzymes, chymostatin and Y-40613, a novel chymase inhibitor, suppressed the enhanced immunoglobulin production, and phenylmethylsulphonyl fluoride, an irreversible inhibitor for serine proteases, totally abolished the enhancing effect.	Phenylmethylsulfonyl Fluoride	223-253	mast cell protease 1-like 1	Rattus norvegicus	22-28
11399668-4	2001	For inhibition of rat brain [(3)H]-AEA metabolism by a series of known FAAH inhibitors, the potencies of the enantiomers of ibuprofen and phenylmethylsulphonyl fluoride (PMSF) were higher at pH 5.28 than at pH 8.37, whereas the reverse was true for oleyl trifluoromethylketone (OTMK) and arachidonoylserotonin.	Phenylmethylsulfonyl Fluoride	138-168	fatty-acid amide hydrolase-like	Rattus norvegicus	71-75
11467830-6	2001	Although the degradation of RT was unaffected by specific proteasome inhibitors, it could be inhibited by PMSF and aprotinin, suggesting the involvement of a serine protease.	Phenylmethylsulfonyl Fluoride	106-110	coagulation factor II, thrombin	Homo sapiens	158-173
11675007-7	2001	The essential function of AUT8 for autophagy is further demonstrated by the lack of accumulation of autophagic vesicles in the vacuoles of aut8 Delta cells starved of nitrogen in the presence of the proteinase B inhibitor phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	222-251	Atg2p	Saccharomyces cerevisiae S288C	26-30
11675007-7	2001	The essential function of AUT8 for autophagy is further demonstrated by the lack of accumulation of autophagic vesicles in the vacuoles of aut8 Delta cells starved of nitrogen in the presence of the proteinase B inhibitor phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	222-251	proteinase B	Saccharomyces cerevisiae S288C	199-211
11485388-6	2001	The disappearance of bcl-x(L) from FL5.12 cells upon MK886 treatment was prevented in a dose-dependent manner by pretreatment with leupeptin, pepstatin, phenylmethylsulfonyl fluoride, or the broad-spectrum caspase inhibitor Boc-D-FMK.	Phenylmethylsulfonyl Fluoride	153-182	BCL2-like 1	Mus musculus	21-29
22061172-6	2001	The serine peptidase inhibitors PMSF and Pefabloc SC suppressed DPP II activity in a high degree, whereas 3, 4-DCI and cysteine peptidase inhibitors exerted little effect.	Phenylmethylsulfonyl Fluoride	32-36	dipeptidyl peptidase 7	Homo sapiens	64-70
11356570-5	2001	This protease activity was able to degrade rabbit hemopexin and human hemopexin, as well as human transferrin and ovalbumin, and may be a due to a serine protease since it was inhibited by phenylmethylsulfonyl fluoride (PMSF) but not by EDTA, leupeptin, pepstatin A or aprotinin.	Phenylmethylsulfonyl Fluoride	189-218	hemopexin	Oryctolagus cuniculus	50-59
11356570-5	2001	This protease activity was able to degrade rabbit hemopexin and human hemopexin, as well as human transferrin and ovalbumin, and may be a due to a serine protease since it was inhibited by phenylmethylsulfonyl fluoride (PMSF) but not by EDTA, leupeptin, pepstatin A or aprotinin.	Phenylmethylsulfonyl Fluoride	189-218	hemopexin	Homo sapiens	70-79
11356570-5	2001	This protease activity was able to degrade rabbit hemopexin and human hemopexin, as well as human transferrin and ovalbumin, and may be a due to a serine protease since it was inhibited by phenylmethylsulfonyl fluoride (PMSF) but not by EDTA, leupeptin, pepstatin A or aprotinin.	Phenylmethylsulfonyl Fluoride	189-218	transferrin	Homo sapiens	98-109
11356570-5	2001	This protease activity was able to degrade rabbit hemopexin and human hemopexin, as well as human transferrin and ovalbumin, and may be a due to a serine protease since it was inhibited by phenylmethylsulfonyl fluoride (PMSF) but not by EDTA, leupeptin, pepstatin A or aprotinin.	Phenylmethylsulfonyl Fluoride	220-224	hemopexin	Oryctolagus cuniculus	50-59
11356570-5	2001	This protease activity was able to degrade rabbit hemopexin and human hemopexin, as well as human transferrin and ovalbumin, and may be a due to a serine protease since it was inhibited by phenylmethylsulfonyl fluoride (PMSF) but not by EDTA, leupeptin, pepstatin A or aprotinin.	Phenylmethylsulfonyl Fluoride	220-224	hemopexin	Homo sapiens	70-79
11356570-5	2001	This protease activity was able to degrade rabbit hemopexin and human hemopexin, as well as human transferrin and ovalbumin, and may be a due to a serine protease since it was inhibited by phenylmethylsulfonyl fluoride (PMSF) but not by EDTA, leupeptin, pepstatin A or aprotinin.	Phenylmethylsulfonyl Fluoride	220-224	transferrin	Homo sapiens	98-109
11353795-10	2001	Phenylmethylsulfonyl fluoride, a FAAH inhibitor that is structurally unrelated to anandamide, inhibited anandamide uptake in RBL-2H3 cells and FAAH-transfected HeLa cells, but not in wild-type HeLa cells.	Phenylmethylsulfonyl Fluoride	0-29	fatty-acid amide hydrolase-like	Rattus norvegicus	33-37
11353795-10	2001	Phenylmethylsulfonyl fluoride, a FAAH inhibitor that is structurally unrelated to anandamide, inhibited anandamide uptake in RBL-2H3 cells and FAAH-transfected HeLa cells, but not in wild-type HeLa cells.	Phenylmethylsulfonyl Fluoride	0-29	fatty-acid amide hydrolase-like	Rattus norvegicus	143-147
11278420-2	2001	Two selective inhibitors of anandamide facilitated transport into cells, VDM11 and VDM13, and two inhibitors of anandamide enzymatic hydrolysis, phenylmethylsulfonyl fluoride and methylarachidonoyl fluorophosphonate, inhibited and enhanced, respectively, the VR1-mediated effect of anandamide, but not of resiniferatoxin or capsaicin.	Phenylmethylsulfonyl Fluoride	145-174	vault RNA 1-1	Homo sapiens	259-262
11171108-2	2001	Anhydrothrombin was prepared from PMSF-inactivated thrombin under alkaline conditions, and the folded anhydrothrombin was successfully recovered after dialysis in the presence of glycerol.	Phenylmethylsulfonyl Fluoride	34-38	coagulation factor II, thrombin	Homo sapiens	7-15
11160333-4	2001	Using radioiodinated DBP as a probe, neutrophils released 70% of previously bound DBP into the extracellular media during a 60-min incubation at 37 degrees C. This was suppressed by serine protease inhibitors (PMSF, Pefabloc SC), but not by metallo- or thiol-protease inhibitors.	Phenylmethylsulfonyl Fluoride	210-214	D-box binding PAR bZIP transcription factor	Homo sapiens	21-24
11160333-4	2001	Using radioiodinated DBP as a probe, neutrophils released 70% of previously bound DBP into the extracellular media during a 60-min incubation at 37 degrees C. This was suppressed by serine protease inhibitors (PMSF, Pefabloc SC), but not by metallo- or thiol-protease inhibitors.	Phenylmethylsulfonyl Fluoride	210-214	D-box binding PAR bZIP transcription factor	Homo sapiens	82-85
11160333-6	2001	Cells treated with PMSF accumulate DBP vs time with over 90% of the protein localized to the plasma membrane.	Phenylmethylsulfonyl Fluoride	19-23	D-box binding PAR bZIP transcription factor	Homo sapiens	35-38
11732624-7	2001	Higher PMSF concentration resulted in lower yield of the 38 kDa band and higher yield of intact RLIP76 from both human and recombinant source.	Phenylmethylsulfonyl Fluoride	7-11	ralA binding protein 1	Homo sapiens	96-102
10801859-3	2000	Although the primary structure of PPT1 contains a serine lipase consensus sequence, the enzyme is insensitive to commonly used serine-modifying reagents phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.	Phenylmethylsulfonyl Fluoride	153-182	palmitoyl-protein thioesterase 1	Homo sapiens	34-38
11137456-5	2000	The endopeptidase PE, with a molecular weight of 45 kDa, was inhibited 100% by EDTA and pOHMB and resistant to PMSF, thyorphan, E64 and phosphoramidon, when we used the mentioned substrates.	Phenylmethylsulfonyl Fluoride	111-115	prolyl endopeptidase	Homo sapiens	18-20
11137461-5	2000	These effects were inhibited selectively by serine protease inhibitors (soybean trypsin inhibitor, di-isopropyl phosphofluoridate, phenylmethanesulfonyl fluoride).	Phenylmethylsulfonyl Fluoride	131-161	complement component 1, s subcomponent 1	Mus musculus	44-59
11105997-7	2000	The purified PAF-AH has an apparent Km value of 4.9 microM and the enzyme activity was inactivated by PMSF and 5,5"-dithiobis-(2-nitrobenzoic acid) (DTNB) and moderately stimulated by dithiothreitol (DTT).	Phenylmethylsulfonyl Fluoride	102-106	PCNA clamp associated factor	Rattus norvegicus	13-16
11015300-4	2000	However, in contrast to the rat brain, phenylmethylsulphonyl fluoride (PMSF) and the enantiomers of ibuprofen had very weak effects on chicken brain FAAH.	Phenylmethylsulfonyl Fluoride	39-69	fatty acid amide hydrolase	Gallus gallus	149-153
11015300-4	2000	However, in contrast to the rat brain, phenylmethylsulphonyl fluoride (PMSF) and the enantiomers of ibuprofen had very weak effects on chicken brain FAAH.	Phenylmethylsulfonyl Fluoride	71-75	fatty acid amide hydrolase	Gallus gallus	149-153
10996026-7	2000	We revealed that T614 and phenylmethyl sulfonyl fluoride (PMSF), a serine protease inhibitor, inhibited Leu-OME mediated killing of THP-1 cells.	Phenylmethylsulfonyl Fluoride	26-56	GLI family zinc finger 2	Homo sapiens	132-137
10996026-7	2000	We revealed that T614 and phenylmethyl sulfonyl fluoride (PMSF), a serine protease inhibitor, inhibited Leu-OME mediated killing of THP-1 cells.	Phenylmethylsulfonyl Fluoride	58-62	GLI family zinc finger 2	Homo sapiens	132-137
11032907-7	2000	The addition of phenylmethylsulfonyl fluoride, a serine protease inhibitor, to a sample of tPA, plgn, and Abeta resulted in a marked reduction of Abeta degradation.	Phenylmethylsulfonyl Fluoride	16-45	amyloid beta precursor protein	Homo sapiens	106-111
11032765-12	2000	Alteration of OP compound-induced nuclear fragmentation or caspase-3 activation by pretreatment with cyclosporin A, Ac-IETD-CHO, or PMSF suggested that OP compound-induced cytotoxicity may be modulated through multiple sites, including mitochondrial permeability pores, receptor-mediated caspase pathways, or serine proteases.	Phenylmethylsulfonyl Fluoride	132-136	caspase 3	Homo sapiens	59-68
10801859-3	2000	Although the primary structure of PPT1 contains a serine lipase consensus sequence, the enzyme is insensitive to commonly used serine-modifying reagents phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.	Phenylmethylsulfonyl Fluoride	184-188	palmitoyl-protein thioesterase 1	Homo sapiens	34-38
10801859-4	2000	In the current paper, we show that the active site serine in PPT1 is modified by a substrate analog of PMSF, hexadecylsulfonylfluoride (HDSF) in a specific and site-directed manner.	Phenylmethylsulfonyl Fluoride	103-107	palmitoyl-protein thioesterase 1	Homo sapiens	61-65
11275256-5	2000	The antibodies recognised AchE and were capable of hydrolysing acetylthiocholine and the larger butyrylthiocholine substrate, and were inactivated by phenylmethylsulphonyl fluoride (PMSF), indicating a serine residue in the active site.	Phenylmethylsulfonyl Fluoride	150-180	acetylcholinesterase (Cartwright blood group)	Homo sapiens	26-30
10994773-9	2000	The protease inhibitors PMSF, aprotinin and leupeptin produced a dose-dependent increase in measurable levels of IL-2 and IL-10 in plasma.	Phenylmethylsulfonyl Fluoride	24-28	interleukin 2	Homo sapiens	113-117
10994773-9	2000	The protease inhibitors PMSF, aprotinin and leupeptin produced a dose-dependent increase in measurable levels of IL-2 and IL-10 in plasma.	Phenylmethylsulfonyl Fluoride	24-28	interleukin 10	Homo sapiens	122-127
11275256-5	2000	The antibodies recognised AchE and were capable of hydrolysing acetylthiocholine and the larger butyrylthiocholine substrate, and were inactivated by phenylmethylsulphonyl fluoride (PMSF), indicating a serine residue in the active site.	Phenylmethylsulfonyl Fluoride	182-186	acetylcholinesterase (Cartwright blood group)	Homo sapiens	26-30
10899613-3	2000	The classification as serine protease was based on the sensitivity towards chymostatin and phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	91-120	subtilisin-like protease SBT1.7	Nicotiana tabacum	22-37
10715157-5	2000	Reported herein is the design and evaluation of a new pTyr mimetic, p-malonylphenylalanine (Pmf), which does not contain phosphorus yet, in Grb2 SH2 domain binding systems, approaches the potency of phosphonate-based pTyr mimetics.	Phenylmethylsulfonyl Fluoride	92-95	growth factor receptor bound protein 2	Homo sapiens	140-144
10856970-0	2000	Radiomodfication of xanthine oxidoreductase system in the liver of mice by phenylmethylsulfonyl fluoride and dithiothreitol.	Phenylmethylsulfonyl Fluoride	75-104	xanthine dehydrogenase	Mus musculus	20-43
10856970-2	2000	In the present work, radiomodification of the XOR system by phenylmethylsulfonyl fluoride (PMSF) and dithiothreitol (DTT) was examined using female Swiss albino mice which were irradiated with gamma rays at a dose rate 0.023 Gy s(-1).	Phenylmethylsulfonyl Fluoride	60-89	xanthine dehydrogenase	Mus musculus	46-49
10856970-2	2000	In the present work, radiomodification of the XOR system by phenylmethylsulfonyl fluoride (PMSF) and dithiothreitol (DTT) was examined using female Swiss albino mice which were irradiated with gamma rays at a dose rate 0.023 Gy s(-1).	Phenylmethylsulfonyl Fluoride	91-95	xanthine dehydrogenase	Mus musculus	46-49
10805370-8	2000	The enhancement of IL-6 and IL-8 production by MCG was abrogated when MCG were pretreated with the serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	125-154	interleukin 6	Homo sapiens	19-23
10805370-8	2000	The enhancement of IL-6 and IL-8 production by MCG was abrogated when MCG were pretreated with the serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	125-154	C-X-C motif chemokine ligand 8	Homo sapiens	28-32
10805370-8	2000	The enhancement of IL-6 and IL-8 production by MCG was abrogated when MCG were pretreated with the serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	156-160	interleukin 6	Homo sapiens	19-23
10805370-8	2000	The enhancement of IL-6 and IL-8 production by MCG was abrogated when MCG were pretreated with the serine protease inhibitor phenylmethylsulfonyl fluoride (PMSF).	Phenylmethylsulfonyl Fluoride	156-160	C-X-C motif chemokine ligand 8	Homo sapiens	28-32
10856970-5	2000	The inhibition of XO activity, restoration of XDH activity, and increase in the XDH/XO ratio upon administration of PMSF were suggestive of irreversible conversion of XDH into XO mediated through serine proteases.	Phenylmethylsulfonyl Fluoride	116-120	xanthine dehydrogenase	Mus musculus	80-83
10856970-5	2000	The inhibition of XO activity, restoration of XDH activity, and increase in the XDH/XO ratio upon administration of PMSF were suggestive of irreversible conversion of XDH into XO mediated through serine proteases.	Phenylmethylsulfonyl Fluoride	116-120	xanthine dehydrogenase	Mus musculus	80-83
10825396-0	2000	Inactivation studies of acetylcholinesterase with phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	50-79	acetylcholinesterase	Mus musculus	24-44
10825396-1	2000	Acetylcholinesterase (AChE), a serine hydrolase, is potentially susceptible to inactivation by phenylmethylsulfonyl fluoride (PMSF) and benzenesulfonyl fluoride (BSF).	Phenylmethylsulfonyl Fluoride	95-124	acetylcholinesterase	Mus musculus	0-20
10825396-1	2000	Acetylcholinesterase (AChE), a serine hydrolase, is potentially susceptible to inactivation by phenylmethylsulfonyl fluoride (PMSF) and benzenesulfonyl fluoride (BSF).	Phenylmethylsulfonyl Fluoride	95-124	acetylcholinesterase	Mus musculus	22-26
10825396-1	2000	Acetylcholinesterase (AChE), a serine hydrolase, is potentially susceptible to inactivation by phenylmethylsulfonyl fluoride (PMSF) and benzenesulfonyl fluoride (BSF).	Phenylmethylsulfonyl Fluoride	126-130	acetylcholinesterase	Mus musculus	0-20
10825396-1	2000	Acetylcholinesterase (AChE), a serine hydrolase, is potentially susceptible to inactivation by phenylmethylsulfonyl fluoride (PMSF) and benzenesulfonyl fluoride (BSF).	Phenylmethylsulfonyl Fluoride	126-130	acetylcholinesterase	Mus musculus	22-26
10825396-5	2000	Inactivation rate constants for T. californica AChE confirmed previous reports that this enzyme is not inactivated by PMSF.	Phenylmethylsulfonyl Fluoride	118-122	acetylcholinesterase	Mus musculus	47-51
10825396-6	2000	Wild-type mouse AChE and mouse mutants Y330F and Y330A all had similar inactivation rate constants with PMSF, implying that the difference between mouse and T. californica AChE at position 330 is not responsible for their differing PMSF sensitivities.	Phenylmethylsulfonyl Fluoride	104-108	acetylcholinesterase	Mus musculus	16-20
10712396-6	2000	Production of soluble LOX-1 was inhibited by PMSF, suggesting that PMSF-sensitive proteases may be involved in this process.	Phenylmethylsulfonyl Fluoride	45-49	oxidized low density lipoprotein receptor 1	Bos taurus	22-27
10776921-10	2000	The enzymatic activity was inhibited by phenylmethylsulfonyl fluoride, suggesting it is a serine protease.	Phenylmethylsulfonyl Fluoride	40-69	coagulation factor II, thrombin	Homo sapiens	90-105
10428468-3	1999	NAPE-PLD is inhibited by the fatty acid aminohydrolase inhibitor MAFP in high concentrations (> or = 100 microM) while PMSF in high concentrations (10 mM) tends to stabilise NAPE-PLD activity.	Phenylmethylsulfonyl Fluoride	122-126	N-acyl phosphatidylethanolamine phospholipase D	Rattus norvegicus	177-185
10569731-7	1999	Among various inhibitors tested, diprotin A and phenylmethylsulfonyl fluoride inhibited the enzymatic activity, suggesting that the enzyme induced by IL-1alpha was DPPIV.	Phenylmethylsulfonyl Fluoride	48-77	interleukin 1 alpha	Homo sapiens	150-159
10569731-7	1999	Among various inhibitors tested, diprotin A and phenylmethylsulfonyl fluoride inhibited the enzymatic activity, suggesting that the enzyme induced by IL-1alpha was DPPIV.	Phenylmethylsulfonyl Fluoride	48-77	dipeptidyl peptidase 4	Homo sapiens	164-169
10564640-8	1999	The angiogenic activity of uPA is significantly inhibited by neutralizing anti-FGF2 antibodies or by pretreatment with phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	119-148	plasminogen activator, urokinase	Homo sapiens	27-30
10569701-10	1999	In addition, the FcgammaR down-regulation is abrogated by phenyl methyl sulfonyl fluoride (PMSF) but not by other protease inhibitors such as pepstatin, thiorphan, phosphoramidon and leupeptin, suggesting a role for serine protease(s) in this process.	Phenylmethylsulfonyl Fluoride	58-89	coagulation factor II, thrombin	Homo sapiens	216-231
10569701-10	1999	In addition, the FcgammaR down-regulation is abrogated by phenyl methyl sulfonyl fluoride (PMSF) but not by other protease inhibitors such as pepstatin, thiorphan, phosphoramidon and leupeptin, suggesting a role for serine protease(s) in this process.	Phenylmethylsulfonyl Fluoride	91-95	coagulation factor II, thrombin	Homo sapiens	216-231
10480954-6	1999	Granzyme K activity was shown to be inhibited by the synthetic compounds Phe-Pro-Arg-chloromethyl ketone, phenylmethylsulfonyl fluoride, PefablocSC, and benzamidine, by the Kunitz-type inhibitor aprotinin and by human blood plasma.	Phenylmethylsulfonyl Fluoride	106-135	granzyme K	Homo sapiens	0-10
10428479-5	1999	One of them appeared whether E-64 or PMSF was added or not, evidently representing a cystatin C/cathepsin L complex.	Phenylmethylsulfonyl Fluoride	37-41	cystatin C	Homo sapiens	85-95
10428479-5	1999	One of them appeared whether E-64 or PMSF was added or not, evidently representing a cystatin C/cathepsin L complex.	Phenylmethylsulfonyl Fluoride	37-41	cathepsin L	Homo sapiens	96-107
10431820-8	1999	Furthermore, the enzyme of CMK cells was much less sensitive to phenylmethylsulfonyl fluoride and methyl arachidonoyl fluorophosphonate potently inhibiting anandamide amidohydrolase, and effectively hydrolyzed palmitoylethanolamide, which was a poor substrate for anandamide amidohydrolase.	Phenylmethylsulfonyl Fluoride	64-93	C-X-C motif chemokine ligand 9	Homo sapiens	27-30
10484747-2	1999	They can cleave the human fibrinogen to release the fibrinopeptide A and fibrinopeptide B with specific activity of 120 and 370 NIH units/mg, respectively; the fibrinogen-clotting activity can be inhibited distinctly by PMSF or DFP or EDTA, but not by heparin.	Phenylmethylsulfonyl Fluoride	220-224	fibrinogen beta chain	Homo sapiens	26-36
10447140-6	1999	Furthermore, this proteolytic activity was inhibited by PMSF (p < 0.001), a specific serine protease inhibitor.	Phenylmethylsulfonyl Fluoride	56-60	coagulation factor II, thrombin	Homo sapiens	88-103
10484747-2	1999	They can cleave the human fibrinogen to release the fibrinopeptide A and fibrinopeptide B with specific activity of 120 and 370 NIH units/mg, respectively; the fibrinogen-clotting activity can be inhibited distinctly by PMSF or DFP or EDTA, but not by heparin.	Phenylmethylsulfonyl Fluoride	220-224	fibrinogen beta chain	Homo sapiens	160-170
10225873-10	1999	The Pet protein induced proteolysis in zymogram gels, and preincubation with the serine protease inhibitor phenylmethylsulfonyl fluoride resulted in complete abrogation of Pet cytopathic effects.	Phenylmethylsulfonyl Fluoride	107-136	coagulation factor II, thrombin	Homo sapiens	81-96
10329783-6	1999	The protease is considered as a serine-type protease and contains metal ion(s) to some extent, as indicated by the fact that its clotting and arginine-esterase activities could be completely inhibited by PMSF and partially inhibited by the chelating agent EDTA, while the thrombin inhibitor heparin had no effect on its clotting activity towards rabbit citrated plasma.	Phenylmethylsulfonyl Fluoride	204-208	prothrombin	Oryctolagus cuniculus	272-280
10340306-0	1999	Phenylmethylsulfonyl fluoride inhibitory effects on acetylcholinesterase of brain and muscle.	Phenylmethylsulfonyl Fluoride	0-29	acetylcholinesterase (Cartwright blood group)	Homo sapiens	52-72
10340306-1	1999	Differential inhibition of brain versus peripheral acetylcholinesterase (AChE) by phenylmethylsulfonyl fluoride (PMSF) suggested that PMSF might preferentially inhibit different AChE molecular forms.	Phenylmethylsulfonyl Fluoride	82-111	acetylcholinesterase (Cartwright blood group)	Homo sapiens	51-71
10340306-1	1999	Differential inhibition of brain versus peripheral acetylcholinesterase (AChE) by phenylmethylsulfonyl fluoride (PMSF) suggested that PMSF might preferentially inhibit different AChE molecular forms.	Phenylmethylsulfonyl Fluoride	82-111	acetylcholinesterase (Cartwright blood group)	Homo sapiens	73-77
10340306-1	1999	Differential inhibition of brain versus peripheral acetylcholinesterase (AChE) by phenylmethylsulfonyl fluoride (PMSF) suggested that PMSF might preferentially inhibit different AChE molecular forms.	Phenylmethylsulfonyl Fluoride	82-111	acetylcholinesterase (Cartwright blood group)	Homo sapiens	178-182
10340306-1	1999	Differential inhibition of brain versus peripheral acetylcholinesterase (AChE) by phenylmethylsulfonyl fluoride (PMSF) suggested that PMSF might preferentially inhibit different AChE molecular forms.	Phenylmethylsulfonyl Fluoride	113-117	acetylcholinesterase (Cartwright blood group)	Homo sapiens	51-71
10340306-1	1999	Differential inhibition of brain versus peripheral acetylcholinesterase (AChE) by phenylmethylsulfonyl fluoride (PMSF) suggested that PMSF might preferentially inhibit different AChE molecular forms.	Phenylmethylsulfonyl Fluoride	113-117	acetylcholinesterase (Cartwright blood group)	Homo sapiens	73-77
10340306-1	1999	Differential inhibition of brain versus peripheral acetylcholinesterase (AChE) by phenylmethylsulfonyl fluoride (PMSF) suggested that PMSF might preferentially inhibit different AChE molecular forms.	Phenylmethylsulfonyl Fluoride	134-138	acetylcholinesterase (Cartwright blood group)	Homo sapiens	51-71
10340306-1	1999	Differential inhibition of brain versus peripheral acetylcholinesterase (AChE) by phenylmethylsulfonyl fluoride (PMSF) suggested that PMSF might preferentially inhibit different AChE molecular forms.	Phenylmethylsulfonyl Fluoride	134-138	acetylcholinesterase (Cartwright blood group)	Homo sapiens	73-77
10340306-1	1999	Differential inhibition of brain versus peripheral acetylcholinesterase (AChE) by phenylmethylsulfonyl fluoride (PMSF) suggested that PMSF might preferentially inhibit different AChE molecular forms.	Phenylmethylsulfonyl Fluoride	134-138	acetylcholinesterase (Cartwright blood group)	Homo sapiens	178-182
10340306-9	1999	These results suggest that PMSF inhibition of AChE is a consequence of a selective inhibition of membrane-associated forms and that the apparent brain selectivity is related to the greater fraction of membrane-associated AChE in brain.	Phenylmethylsulfonyl Fluoride	27-31	acetylcholinesterase (Cartwright blood group)	Homo sapiens	46-50
10340306-9	1999	These results suggest that PMSF inhibition of AChE is a consequence of a selective inhibition of membrane-associated forms and that the apparent brain selectivity is related to the greater fraction of membrane-associated AChE in brain.	Phenylmethylsulfonyl Fluoride	27-31	acetylcholinesterase (Cartwright blood group)	Homo sapiens	221-225
10073755-8	1999	Elastin substrate zymography revealed two phenylmethylsulfonyl fluoride-inhibitable elastin-degrading enzyme activities in the aortic tissue homogenate that corresponded to human neutrophil elastase (approximately 30 kDa) and its stable complex with alpha 1-proteinase inhibitor (approximately 80 kDa), but no activity attributable to Pseudomonas elastase, a 33-kDa metal-dependent enzyme.	Phenylmethylsulfonyl Fluoride	42-71	elastin	Homo sapiens	0-7
10461681-4	1999	However, the serine protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), significantly reduced these chemokine productions by macrophages stimulated with NE.	Phenylmethylsulfonyl Fluoride	40-69	elastase, neutrophil expressed	Rattus norvegicus	159-161
10461681-4	1999	However, the serine protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), significantly reduced these chemokine productions by macrophages stimulated with NE.	Phenylmethylsulfonyl Fluoride	71-75	elastase, neutrophil expressed	Rattus norvegicus	159-161
9931444-5	1999	ApoB degradation was decreased to 50% when potent proteasome inhibitors, clasto-lactacystin beta-lactone (10 microM) or MG-132 (50 microM), were added to the reaction mixture, but was not affected by the cysteine protease inhibitor, E-64, or the serine protease inhibitor, phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	273-302	apolipoprotein B	Homo sapiens	0-4
10426227-3	1999	Previous work has shown that CE activity could be inhibited by the serine protease/esterase inhibitor, phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	103-132	carboxyl ester lipase	Homo sapiens	29-31
10426227-3	1999	Previous work has shown that CE activity could be inhibited by the serine protease/esterase inhibitor, phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	103-132	coagulation factor II, thrombin	Homo sapiens	67-82
10073755-8	1999	Elastin substrate zymography revealed two phenylmethylsulfonyl fluoride-inhibitable elastin-degrading enzyme activities in the aortic tissue homogenate that corresponded to human neutrophil elastase (approximately 30 kDa) and its stable complex with alpha 1-proteinase inhibitor (approximately 80 kDa), but no activity attributable to Pseudomonas elastase, a 33-kDa metal-dependent enzyme.	Phenylmethylsulfonyl Fluoride	42-71	elastin	Homo sapiens	84-91
10073755-8	1999	Elastin substrate zymography revealed two phenylmethylsulfonyl fluoride-inhibitable elastin-degrading enzyme activities in the aortic tissue homogenate that corresponded to human neutrophil elastase (approximately 30 kDa) and its stable complex with alpha 1-proteinase inhibitor (approximately 80 kDa), but no activity attributable to Pseudomonas elastase, a 33-kDa metal-dependent enzyme.	Phenylmethylsulfonyl Fluoride	42-71	elastase, neutrophil expressed	Homo sapiens	179-198
9856830-4	1998	The enzyme responsible was inhibited by the serine protease inhibitor phenyl-methylsulfonyl fluoride, but was not inhibited by alpha1-anti-trypsin, an inhibitor of neutrophil elastase, by alpha2-anti-plasmin, an inhibitor of plasmin, or by the matrix metalloprotease inhibitor 1,10 phenanthroline.	Phenylmethylsulfonyl Fluoride	70-100	coagulation factor II, thrombin	Homo sapiens	44-59
9794247-4	1998	Phenylmethylsulphonyl fluoride, lactacystin, and carbobenzoxyleucylleucylnorvaline also enhance prostacyclin production after incubation with interleukin-1beta and transforming growth factor-alpha.	Phenylmethylsulfonyl Fluoride	0-30	interleukin 1 beta	Rattus norvegicus	142-159
9794247-6	1998	Cells, treated with phenylmethylsulphonyl fluoride, TPA, interleukin-1beta, lactacystin or the peptide aldehydes exhibit increased prostaglandin endoperoxide G/H synthase activity.	Phenylmethylsulfonyl Fluoride	20-50	interleukin 1 beta	Rattus norvegicus	57-74
9808342-10	1998	Tenascin-C breakdown was inhibited by PMSF and SKALP/elafin, and we therefore conclude that leucocyte elastase and possibly other serine proteinases are the tenascin-C-degrading enzymes in ulcer exudate.	Phenylmethylsulfonyl Fluoride	38-42	tenascin C	Homo sapiens	0-10
9695914-5	1998	SepA hydrolysed several of these substrates and the activity was inhibited by PMSF.	Phenylmethylsulfonyl Fluoride	78-82	SepA	Shigella flexneri	0-4
9643362-5	1998	FRTL5 cells were able to convert lyso-PAF to PAF especially when they were stimulated by ionophore A23187 in the presence of [3H]Iyso-PAF and phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	142-171	PCNA clamp associated factor	Rattus norvegicus	38-41
9643362-5	1998	FRTL5 cells were able to convert lyso-PAF to PAF especially when they were stimulated by ionophore A23187 in the presence of [3H]Iyso-PAF and phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	142-171	PCNA clamp associated factor	Rattus norvegicus	45-48
9643362-5	1998	FRTL5 cells were able to convert lyso-PAF to PAF especially when they were stimulated by ionophore A23187 in the presence of [3H]Iyso-PAF and phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	142-171	PCNA clamp associated factor	Rattus norvegicus	45-48
10073755-6	1999	Elastin-degrading enzyme activity in the aortic tissue homogenate of this patient was abolished by the serine protease inhibitor, phenylmethylsulfonyl fluoride, but it was not suppressed by the metalloproteinase inhibitor, ethylenediamine tetraacetic acid (EDTA).	Phenylmethylsulfonyl Fluoride	130-159	elastin	Homo sapiens	0-7
10073755-7	1999	In contrast, elastin-degrading enzyme activity in the bacterial-conditioned medium was decreased by about half by both phenylmethylsulfonyl fluoride and EDTA.	Phenylmethylsulfonyl Fluoride	119-148	elastin	Homo sapiens	13-20
9820852-4	1998	The activity of sK1 upon rat plasma kininogen was strongly inhibited by the serine proteinase inhibitors phenylmethanesulfonyl fluoride, aprotinin or soybean trypsin inhibitor, but not by ethylenediaminetetraacetic acid or sodium tetrathionate.	Phenylmethylsulfonyl Fluoride	105-135	sphingosine kinase 1	Rattus norvegicus	16-19
10557188-7	1998	RESULTS: Part 1: IL-8 level in the PMSF samples was significantly greater than that in the control group (3.01 +/- 5.79 mg/L vs 0.05 +/- 0.15 mg/L, respectively P < .001).	Phenylmethylsulfonyl Fluoride	35-39	C-X-C motif chemokine ligand 8	Homo sapiens	17-21
9620662-9	1998	The down-regulation of the three antigens CD53, CD43, and CD44 could be inhibited by phenylmethylsulfonyl fluoride, suggesting that CD53 antigen down-regulation is the result of the activation of a proteolytic mechanism.	Phenylmethylsulfonyl Fluoride	85-114	CD53 molecule	Homo sapiens	42-46
9620662-9	1998	The down-regulation of the three antigens CD53, CD43, and CD44 could be inhibited by phenylmethylsulfonyl fluoride, suggesting that CD53 antigen down-regulation is the result of the activation of a proteolytic mechanism.	Phenylmethylsulfonyl Fluoride	85-114	sialophorin	Homo sapiens	48-52
9620662-9	1998	The down-regulation of the three antigens CD53, CD43, and CD44 could be inhibited by phenylmethylsulfonyl fluoride, suggesting that CD53 antigen down-regulation is the result of the activation of a proteolytic mechanism.	Phenylmethylsulfonyl Fluoride	85-114	CD44 molecule (Indian blood group)	Homo sapiens	58-62
9620662-9	1998	The down-regulation of the three antigens CD53, CD43, and CD44 could be inhibited by phenylmethylsulfonyl fluoride, suggesting that CD53 antigen down-regulation is the result of the activation of a proteolytic mechanism.	Phenylmethylsulfonyl Fluoride	85-114	CD53 molecule	Homo sapiens	132-136
11774414-7	1998	RESULTS: (1) IL-8 level in the PMSF-added samples were significantly greater than that in the control group(3.01 mg/L +/- 5.79 mg/L vs 0.05 mg/L +/- 0.15 mg/L, P < 0.001).	Phenylmethylsulfonyl Fluoride	31-35	C-X-C motif chemokine ligand 8	Homo sapiens	13-17
9525960-16	1998	Preincubation of the enzyme with 1 mM N-ethylmaleimide, 1 mM phenylmethylsulfonyl fluoride, or 3.1 microM bromoenol lactone, a potent inhibitor of cytosolic Ca2+-independent phospholipase A2, had no significant effect on the enzyme activity.	Phenylmethylsulfonyl Fluoride	61-90	LOC104974671	Bos taurus	174-190
9443854-6	1998	Reaction of microsomes with either PAX or PMSF and then with EC exacerbated the reduction (285%) of NDMA demethylation, and this loss corresponded to decreases in immunodetectable CYP2E1 content.	Phenylmethylsulfonyl Fluoride	42-46	cytochrome P450, family 2, subfamily e, polypeptide 1	Mus musculus	180-186
9538257-9	1998	Myonase is completely inhibited by such serine proteinase inhibitors as chymostatin, diisopropylfluorophosphate and phenylmethylsulfonyl fluoride, but not by p-tosyl-L-phenylalanine chloromethyl ketone, p-tosyl-L-lysine chloromethyl ketone, pepstatin, E-64, EDTA, and o-phenanthroline.	Phenylmethylsulfonyl Fluoride	116-145	mast cell protease 4	Mus musculus	0-7
9500517-10	1998	Enzymatically active as well as PMSF-blocked conventionally purified proteinase 3 interfered with phorbol myristate acetate-induced superoxide release.	Phenylmethylsulfonyl Fluoride	32-36	proteinase 3	Homo sapiens	69-81
9453629-11	1998	Pretreatment with the proteinase inhibitor phenylmethylsulfonyl fluoride, which had previously been found to block T. denticola"s degradation of endogenous fibronectin and detachment of HGF from the extracellular matrix, had little effect on F-actin stress fiber disruption and the IP response.	Phenylmethylsulfonyl Fluoride	43-72	hepatocyte growth factor	Homo sapiens	186-189
9613710-7	1998	However, 0.5 mM PMSF enhanced 1.7-fold (p < 0.002) [Ca2+]i rise after challenge with FMLP while did not affect significantly Ca2+ response to PAF and LTB4.	Phenylmethylsulfonyl Fluoride	16-20	formyl peptide receptor 1	Homo sapiens	88-92
9613710-8	1998	The stimulatory effect of PMSF after addition of FMLP was dependent on increased Ca2+ influx from extracellular space.	Phenylmethylsulfonyl Fluoride	26-30	formyl peptide receptor 1	Homo sapiens	49-53
9536127-4	1998	The ability to augment NK cytotoxicity was markedly reduced in the presence of the inhibitor, phenylmethanesulphonyl fluoride (PMSF) or chymostatin, demonstrating that the proteolytic activity of cathepsin G is essential for the induction of NK cytotoxicity.	Phenylmethylsulfonyl Fluoride	94-125	cathepsin G	Homo sapiens	196-207
9536127-4	1998	The ability to augment NK cytotoxicity was markedly reduced in the presence of the inhibitor, phenylmethanesulphonyl fluoride (PMSF) or chymostatin, demonstrating that the proteolytic activity of cathepsin G is essential for the induction of NK cytotoxicity.	Phenylmethylsulfonyl Fluoride	127-131	cathepsin G	Homo sapiens	196-207
10942971-9	1998	PMSF, a specific inhibitor for serine proteases and mammalian acetylcholinesterase, completely inhibited the formation of flumazenil -acid and the flumazenil methylester at a concentration of 100 microM.	Phenylmethylsulfonyl Fluoride	0-4	acetylcholinesterase (Cartwright blood group)	Homo sapiens	62-82
9400368-0	1997	Crystal structure of phenylmethanesulfonyl fluoride-treated human chymase at 1.9 A.	Phenylmethylsulfonyl Fluoride	21-51	chymase 1	Homo sapiens	66-73
9413546-3	1997	Certain esterases inhibitors such as phenylmethylsulfonyl fluoride (PMSF), can also irreversibly inhibit P-NTE and by this mechanism PMSF "protects" from further effect of neuropathic OPs.	Phenylmethylsulfonyl Fluoride	37-66	patatin like phospholipase domain containing 6	Gallus gallus	107-110
9409763-3	1997	Phenylmethylsulfonyl fluoride was shown to be a very effective irreversible inhibitor, completely inactivating the penicillin acylase from A. faecalis in a few minutes at micromolar concentrations; this compound was used for enzyme active site titration.	Phenylmethylsulfonyl Fluoride	0-29	penicillin acylase family protein	Alcaligenes faecalis	115-133
9413546-3	1997	Certain esterases inhibitors such as phenylmethylsulfonyl fluoride (PMSF), can also irreversibly inhibit P-NTE and by this mechanism PMSF "protects" from further effect of neuropathic OPs.	Phenylmethylsulfonyl Fluoride	68-72	patatin like phospholipase domain containing 6	Gallus gallus	107-110
9413546-3	1997	Certain esterases inhibitors such as phenylmethylsulfonyl fluoride (PMSF), can also irreversibly inhibit P-NTE and by this mechanism PMSF "protects" from further effect of neuropathic OPs.	Phenylmethylsulfonyl Fluoride	133-137	patatin like phospholipase domain containing 6	Gallus gallus	107-110
9413546-14	1997	It could be a good candidate to contain the target of the promotion effect of PMSF as well as the S-NTE activity that is also PMSF sensitive.	Phenylmethylsulfonyl Fluoride	126-130	patatin like phospholipase domain containing 6	Gallus gallus	100-103
9344892-8	1997	Alterations in immunodetectable CYP2E1 protein levels were not apparent in microsomes incubated with EC alone, but the amounts were decreased in reactions with EC in conjunction with either PAX or PMSF.	Phenylmethylsulfonyl Fluoride	197-201	cytochrome P450 family 2 subfamily E member 1	Homo sapiens	32-38
9344854-10	1997	This activity was inhibited by FAAH selective inhibitors arachidonoyltrifluoromethylketone and methylarachidonoylfluorophosphonate, as well as by an excess of anandamide, and by PMSF at the same concentration which increased oleamide formation in intact cells.	Phenylmethylsulfonyl Fluoride	178-182	fatty acid amide hydrolase	Mus musculus	31-35
9366034-8	1997	The enzyme"s amidolytic activity, with a pH optimum at 9.0, was inhibited by aprotinin, benzamidine, and phenylmethanesulphonyl fluoride, but not by elastatinal, soybean trypsin inhibitor, or limabean trypsin inhibitor.	Phenylmethylsulfonyl Fluoride	105-136	kunitz trypsin protease inhibitor	Glycine max	201-218
9323582-8	1997	In contrast to native LPL, heat inactivated or phenylmethylsulfonyl fluoride (PMSF)-treated LPL did not increase monocyte adhesion to BAEC.	Phenylmethylsulfonyl Fluoride	47-76	lipoprotein lipase	Homo sapiens	92-95
9323582-8	1997	In contrast to native LPL, heat inactivated or phenylmethylsulfonyl fluoride (PMSF)-treated LPL did not increase monocyte adhesion to BAEC.	Phenylmethylsulfonyl Fluoride	78-82	lipoprotein lipase	Homo sapiens	92-95
9242229-6	1997	DFP and/or PMSF caused > 88% brain NTE inhibition in all treated groups, compared to control.	Phenylmethylsulfonyl Fluoride	11-15	patatin like phospholipase domain containing 6	Gallus gallus	38-41
9303560-3	1997	Two crystal forms of PMSF-treated chymase were optimized.	Phenylmethylsulfonyl Fluoride	21-25	chymase 1	Homo sapiens	34-41
9202171-7	1997	The enzymatic activity responsible for proEGF nicking was inhibited by divalent heavy metal ions (Cu2+ or Zn2+) and several protease inhibitors (aprotinin, PMSF, leupeptin, soybean trypsin inhibitor), suggesting that proEGF is processed by kallikrein-like serine proteases present in the membrane preparations.	Phenylmethylsulfonyl Fluoride	156-160	kunitz trypsin protease inhibitor	Glycine max	181-198
9274811-3	1997	Phenylmethylsulfonyl fluoride (PMSF) was used to prevent proteinase degradation, DNase and RNase were used to degrade prawn DNA and RNA respectively.	Phenylmethylsulfonyl Fluoride	0-29	endogenous retrovirus group K member 10	Homo sapiens	57-67
9274811-3	1997	Phenylmethylsulfonyl fluoride (PMSF) was used to prevent proteinase degradation, DNase and RNase were used to degrade prawn DNA and RNA respectively.	Phenylmethylsulfonyl Fluoride	31-35	endogenous retrovirus group K member 10	Homo sapiens	57-67
9184073-7	1997	From a cocktail of proteinase inhibitors, PMSF was the most active in suppressing fibronectin proteolysis.	Phenylmethylsulfonyl Fluoride	42-46	fibronectin 1	Homo sapiens	82-93
9241533-7	1997	Inhibitors of serine proteases, like phenylmethylsulphonyl fluoride (PMSF), benzamidine and 3, 4-dichloroisocoumarin, blocked the PMA-mediated cleavage of CD43.	Phenylmethylsulfonyl Fluoride	37-67	sialophorin	Homo sapiens	155-159
9241533-7	1997	Inhibitors of serine proteases, like phenylmethylsulphonyl fluoride (PMSF), benzamidine and 3, 4-dichloroisocoumarin, blocked the PMA-mediated cleavage of CD43.	Phenylmethylsulfonyl Fluoride	69-73	sialophorin	Homo sapiens	155-159
9187302-5	1997	Inhibition of proteases by 1 mM leupeptin/PMSF improves the response time to HDL3, with a DAG peak at 2-3 min.	Phenylmethylsulfonyl Fluoride	42-46	HDL3	Homo sapiens	77-81
9193923-1	1997	Phenylmethylsulfonyl fluoride (PMSF), a nonneuropathic inhibitor of neurotoxic esterase (NTE), is a known potentiator of organophosphorus-induced delayed neurotoxicity (OPIDN).	Phenylmethylsulfonyl Fluoride	0-29	patatin like phospholipase domain containing 6	Gallus gallus	89-92
9247605-7	1997	Phenylmethylsulfonyl fluoride inhibited this apparent protease activity against vimentin, suggesting the enzyme involved to be a serine protease.	Phenylmethylsulfonyl Fluoride	0-29	vimentin	Homo sapiens	80-88
9193923-1	1997	Phenylmethylsulfonyl fluoride (PMSF), a nonneuropathic inhibitor of neurotoxic esterase (NTE), is a known potentiator of organophosphorus-induced delayed neurotoxicity (OPIDN).	Phenylmethylsulfonyl Fluoride	31-35	patatin like phospholipase domain containing 6	Gallus gallus	89-92
9096890-9	1997	PMSF (1 mM, serine protease inhibitor) inhibited degradation of IGFBP-2 and to a lesser extent IGFBP-4, but not IGFBP-5.	Phenylmethylsulfonyl Fluoride	0-4	insulin like growth factor binding protein 2	Sus scrofa	64-71
9505357-1	1997	Phenylmethylsulfonyl fluoride (PMSF)-inhibited carboxypeptidase (CP) is recently described exopeptidase, that cleaved arginine residues from C-terminal of enkephalin synthetic analogs.	Phenylmethylsulfonyl Fluoride	0-29	proenkephalin	Rattus norvegicus	155-165
9505357-1	1997	Phenylmethylsulfonyl fluoride (PMSF)-inhibited carboxypeptidase (CP) is recently described exopeptidase, that cleaved arginine residues from C-terminal of enkephalin synthetic analogs.	Phenylmethylsulfonyl Fluoride	31-35	proenkephalin	Rattus norvegicus	155-165
9096890-9	1997	PMSF (1 mM, serine protease inhibitor) inhibited degradation of IGFBP-2 and to a lesser extent IGFBP-4, but not IGFBP-5.	Phenylmethylsulfonyl Fluoride	0-4	insulin like growth factor binding protein 4	Sus scrofa	95-102
9071984-13	1997	The degradation of 44-46 kDa IGFBP-3 in the modified zymographic assay was inhibited by phenylmethylsulfonyl fluoride, EDTA, and aprotinin, but not by leupeptin.	Phenylmethylsulfonyl Fluoride	88-117	insulin-like growth factor binding protein 3	Rattus norvegicus	29-36
9043638-3	1997	At 10(-5) M final concentration PMSF reduced AChE activity to 50% after 24 hours of storage.	Phenylmethylsulfonyl Fluoride	32-36	acetylcholinesterase	Ovis aries	45-49
9226766-6	1997	PMSF and p-BPB decreased the catabolism of PAF by freshly isolated marrow cells, but not by stromal cell cultures.	Phenylmethylsulfonyl Fluoride	0-4	PCNA clamp associated factor	Homo sapiens	43-46
9030219-1	1997	The serine protease inhibitor phenylmethylsulfonyl fluoride is shown to cause partial inhibition of bilitranslocase transport activity in rat liver plasma membrane vesicles.	Phenylmethylsulfonyl Fluoride	30-59	ceruloplasmin	Rattus norvegicus	100-115
9030219-6	1997	Tentatively, the target for phenylmethylsulfonyl fluoride on bilitranslocase is identified as a recognition site for the physiological substrates.	Phenylmethylsulfonyl Fluoride	28-57	ceruloplasmin	Rattus norvegicus	61-76
9043638-7	1997	A more complex cocktail of inhibitors including several commonly used peptides decreased AChE activity only if PMSF or EDTA were present in the mixtures.	Phenylmethylsulfonyl Fluoride	111-115	acetylcholinesterase	Ovis aries	89-93
8597587-8	1996	Thus, the large effect of PMSF on arachidonic acid release can be accounted for if much of the fatty acid formation arose from the sequential sn-1 and sn-2 deacylation of diacyl-PC by phospholipase A1 and lysophospholipase A2.	Phenylmethylsulfonyl Fluoride	26-30	solute carrier family 38 member 5	Bos taurus	151-155
9313830-6	1997	The addition of the protease inhibitor phenylmethanesulphonyl fluoride during fMLP activation improved the upregulation of CD35 significantly more in neonates, but not CD55 or CD59.	Phenylmethylsulfonyl Fluoride	39-70	complement C3b/C4b receptor 1 (Knops blood group)	Homo sapiens	123-127
9000539-4	1997	Phenylmethylsulfonyl fluoride (PMSF) -inhibited cathepsin G also bound to lymphocytes, although both the number of binding sites and the affinity were less than those of native cathepsin G.	Phenylmethylsulfonyl Fluoride	0-29	cathepsin G	Homo sapiens	48-59
9000539-4	1997	Phenylmethylsulfonyl fluoride (PMSF) -inhibited cathepsin G also bound to lymphocytes, although both the number of binding sites and the affinity were less than those of native cathepsin G.	Phenylmethylsulfonyl Fluoride	0-29	cathepsin G	Homo sapiens	177-188
9000539-4	1997	Phenylmethylsulfonyl fluoride (PMSF) -inhibited cathepsin G also bound to lymphocytes, although both the number of binding sites and the affinity were less than those of native cathepsin G.	Phenylmethylsulfonyl Fluoride	31-35	cathepsin G	Homo sapiens	48-59
9000539-4	1997	Phenylmethylsulfonyl fluoride (PMSF) -inhibited cathepsin G also bound to lymphocytes, although both the number of binding sites and the affinity were less than those of native cathepsin G.	Phenylmethylsulfonyl Fluoride	31-35	cathepsin G	Homo sapiens	177-188
9000539-5	1997	The binding of cathepsin G to lymphocytes showed cooperativity, but that of PMSF-inhibited cathepsin G did not.	Phenylmethylsulfonyl Fluoride	76-80	cathepsin G	Homo sapiens	91-102
9000539-6	1997	PMSF-inhibited cathepsin G was able to partially displace bound cathepsin G.	Phenylmethylsulfonyl Fluoride	0-4	cathepsin G	Homo sapiens	15-26
9000539-6	1997	PMSF-inhibited cathepsin G was able to partially displace bound cathepsin G.	Phenylmethylsulfonyl Fluoride	0-4	cathepsin G	Homo sapiens	64-75
8995843-9	1996	HCN yielded strong cross peaks after 18 h on a 2.0 mM sample of phenylmethanesulfonyl fluoride (PMSF)-inhibited alpha-lytic protease (MW = 19.8 kD) at pH 4.4.	Phenylmethylsulfonyl Fluoride	64-94	metastasis associated lung adenocarcinoma transcript 1	Homo sapiens	0-3
8995843-9	1996	HCN yielded strong cross peaks after 18 h on a 2.0 mM sample of phenylmethanesulfonyl fluoride (PMSF)-inhibited alpha-lytic protease (MW = 19.8 kD) at pH 4.4.	Phenylmethylsulfonyl Fluoride	96-100	metastasis associated lung adenocarcinoma transcript 1	Homo sapiens	0-3
8675297-6	1996	Proteolytic attack of the C5aR by enzymes from the P. gingivalis vesicles was inhibited by TPCK (tolylsullonyl phenylalanyl chloromethyl ketone), PMSF (phenylmethylsulfonyl fluoride), and dichloroisocoumarin, suggesting that serine proteinases are primarily responsible for this degradative activity.	Phenylmethylsulfonyl Fluoride	146-150	complement C5a receptor 1	Homo sapiens	26-30
8675297-6	1996	Proteolytic attack of the C5aR by enzymes from the P. gingivalis vesicles was inhibited by TPCK (tolylsullonyl phenylalanyl chloromethyl ketone), PMSF (phenylmethylsulfonyl fluoride), and dichloroisocoumarin, suggesting that serine proteinases are primarily responsible for this degradative activity.	Phenylmethylsulfonyl Fluoride	152-181	complement C5a receptor 1	Homo sapiens	26-30
8761944-8	1996	Mixing experiments indicated that urine samples with aberrant OPN contain proteases inhibitable with phenylmethylsulfonyl fluoride.	Phenylmethylsulfonyl Fluoride	101-130	secreted phosphoprotein 1	Homo sapiens	62-65
8992314-5	1996	The deamidation of AF1 was inhibited by phenylmethylsulfonyl fluoride, p-chloromercuricphenylsulfonate and mercuric chloride, indicating that the deamidase enzyme is a serine protease with a requirement for a free thiol group for activity.	Phenylmethylsulfonyl Fluoride	40-69	interferon gamma receptor 2	Homo sapiens	19-22
8898953-7	1996	Phenylmethylsulfonylfluoride, a serine protease inhibitor, has a negative effect on both TNF-alpha degradation and sTNFR-p75 release by THP-1 cells.	Phenylmethylsulfonyl Fluoride	0-28	tumor necrosis factor	Homo sapiens	89-98
8898953-7	1996	Phenylmethylsulfonylfluoride, a serine protease inhibitor, has a negative effect on both TNF-alpha degradation and sTNFR-p75 release by THP-1 cells.	Phenylmethylsulfonyl Fluoride	0-28	GLI family zinc finger 2	Homo sapiens	136-141
8526865-7	1995	MAGL had no, or negligible, activity towards tri-oleoylglycerol, di-oleoylglycerol, oleoylcholesterol, oleoyl-CoA and phosphatidylcholine; it was inhibited by di-isopropylfluorophosphate, PMSF and diethyl p-nitrophenyl phosphate, suggesting that MAGL is a serine hydrolase.	Phenylmethylsulfonyl Fluoride	188-192	monoglyceride lipase	Homo sapiens	0-4
8543371-6	1995	Pretreatment of neutrophils with the serine protease inhibitors PMSF or 3,4-DCI significantly reduced chemotaxis to C5a, fMLP and IL-8.	Phenylmethylsulfonyl Fluoride	64-68	coagulation factor II, thrombin	Homo sapiens	37-52
8543371-6	1995	Pretreatment of neutrophils with the serine protease inhibitors PMSF or 3,4-DCI significantly reduced chemotaxis to C5a, fMLP and IL-8.	Phenylmethylsulfonyl Fluoride	64-68	complement C5a receptor 1	Homo sapiens	116-119
8543371-6	1995	Pretreatment of neutrophils with the serine protease inhibitors PMSF or 3,4-DCI significantly reduced chemotaxis to C5a, fMLP and IL-8.	Phenylmethylsulfonyl Fluoride	64-68	formyl peptide receptor 1	Homo sapiens	121-125
8543371-6	1995	Pretreatment of neutrophils with the serine protease inhibitors PMSF or 3,4-DCI significantly reduced chemotaxis to C5a, fMLP and IL-8.	Phenylmethylsulfonyl Fluoride	64-68	C-X-C motif chemokine ligand 8	Homo sapiens	130-134
7543917-5	1995	In contrast, PMSF (1 mM) inhibited CCK and bombesin stimulated amylase release, suggesting a covalent interaction with this inhibitor and CCK or bombesin receptors.	Phenylmethylsulfonyl Fluoride	13-17	cholecystokinin	Homo sapiens	35-38
7543917-5	1995	In contrast, PMSF (1 mM) inhibited CCK and bombesin stimulated amylase release, suggesting a covalent interaction with this inhibitor and CCK or bombesin receptors.	Phenylmethylsulfonyl Fluoride	13-17	gastrin releasing peptide	Homo sapiens	43-51
7543917-5	1995	In contrast, PMSF (1 mM) inhibited CCK and bombesin stimulated amylase release, suggesting a covalent interaction with this inhibitor and CCK or bombesin receptors.	Phenylmethylsulfonyl Fluoride	13-17	cholecystokinin	Homo sapiens	138-141
7543917-5	1995	In contrast, PMSF (1 mM) inhibited CCK and bombesin stimulated amylase release, suggesting a covalent interaction with this inhibitor and CCK or bombesin receptors.	Phenylmethylsulfonyl Fluoride	13-17	gastrin releasing peptide	Homo sapiens	145-153
7756277-2	1995	The active-site serine of thrombin was modified to dehydroalanine by promoting the beta-elimination of phenylmethylsulfonic acid from phenylmethylsulfonyl fluoride-inactivated thrombin under conditions in which the enzyme is unfolded.	Phenylmethylsulfonyl Fluoride	134-163	coagulation factor II, thrombin	Homo sapiens	26-34
12228492-8	1995	Furthermore, when the protease inhibitor phenylmethylsulfonyl fluoride was omitted from the purification procedure, GAD activity was insensitive to Ca2+/CaM but was similar in magnitude to CaM-stimulated activity.	Phenylmethylsulfonyl Fluoride	41-70	glutamate decarboxylase	Glycine max	116-119
7756277-2	1995	The active-site serine of thrombin was modified to dehydroalanine by promoting the beta-elimination of phenylmethylsulfonic acid from phenylmethylsulfonyl fluoride-inactivated thrombin under conditions in which the enzyme is unfolded.	Phenylmethylsulfonyl Fluoride	134-163	coagulation factor II, thrombin	Homo sapiens	176-184
7588391-5	1995	Proteinase inhibitors (PMSF and 1,10 phenantroline) inhibited steroid synthesis induced by ACTH and 8-Br-cAMP in intact cells.	Phenylmethylsulfonyl Fluoride	23-27	proopiomelanocortin	Homo sapiens	91-95
8533140-5	1995	When the insulin B-chain was incubated with crude venom previously treated with 2.5 mM PMSF, the Ala14-Leu15 bond was also rapidly cleaved.	Phenylmethylsulfonyl Fluoride	87-91	insulin	Bos taurus	9-16
7546247-14	1995	At pH 3.7 and 37 degrees C, a significant activation of latent TGF-beta 1 was achieved after an incubation of only 15 min, reached the maximum at 120 min, and the activated TGF-beta 1 remained relatively stable for at least 24 h. The activation was not inhibitable by a series of protease inhibitors examined, alone or in combination (e.g., phenyl-methylsulfonyl fluoride, E-64, pepstatin, leupeptin, ethylenediamine tetraacetic acid).	Phenylmethylsulfonyl Fluoride	341-371	transforming growth factor beta 1	Homo sapiens	63-73
7546247-14	1995	At pH 3.7 and 37 degrees C, a significant activation of latent TGF-beta 1 was achieved after an incubation of only 15 min, reached the maximum at 120 min, and the activated TGF-beta 1 remained relatively stable for at least 24 h. The activation was not inhibitable by a series of protease inhibitors examined, alone or in combination (e.g., phenyl-methylsulfonyl fluoride, E-64, pepstatin, leupeptin, ethylenediamine tetraacetic acid).	Phenylmethylsulfonyl Fluoride	341-371	transforming growth factor beta 1	Homo sapiens	173-183
7759568-8	1995	Similarly to rabbit brain endooligopeptidase A, the PC12 endooligopeptidase A-like activity was enhanced by DTT, totally inhibited by DTNB and 1-10 Phenanthroline, partially inhibited by cFP-AAF-pAb, and not affected by PMSF.	Phenylmethylsulfonyl Fluoride	220-224	nudE neurodevelopment protein 1-like 1	Rattus norvegicus	26-46
7759568-8	1995	Similarly to rabbit brain endooligopeptidase A, the PC12 endooligopeptidase A-like activity was enhanced by DTT, totally inhibited by DTNB and 1-10 Phenanthroline, partially inhibited by cFP-AAF-pAb, and not affected by PMSF.	Phenylmethylsulfonyl Fluoride	220-224	nudE neurodevelopment protein 1-like 1	Rattus norvegicus	57-77
7714663-11	1995	Among the protease inhibitors, only two, diprotin-A and phenylmethylsulfonyl fluoride (PMSA), could inhibit DPPIV activity.	Phenylmethylsulfonyl Fluoride	56-85	dipeptidyl peptidase 4	Homo sapiens	108-113
8572929-16	1995	The lack of full protection might be explained by the slow kinetics of TPP, which would cause substantial NTE inhibition when PMSF effects on NTE had subsided.	Phenylmethylsulfonyl Fluoride	126-130	patatin like phospholipase domain containing 6	Gallus gallus	142-145
7528219-11	1994	In contrast, phenylmethylsulfonyl fluoride-inactivated 125I-plasmin retained its binding activity over 24 h, and degradative fragments were not present in the conditioned media.	Phenylmethylsulfonyl Fluoride	13-42	plasminogen	Homo sapiens	60-67
7654338-6	1994	Indeed, cotreatment of thymocytes with the nonspecific serine protease inhibitor phenylmethylsulphonyl fluoride was able to partially protect the stability of the DNA-binding proteins and alter the expression of the c-fos and c-jun genes but did not inhibit apoptosis.	Phenylmethylsulfonyl Fluoride	81-111	Fos proto-oncogene, AP-1 transcription factor subunit	Rattus norvegicus	216-221
8068009-10	1994	The prostate rK9 isoenzymes were, like submandibular-gland rK9, inhibited by soya-bean trypsin inhibitor but not by aprotinin, and were classified as serine proteases as they were inhibited by phenylmethanesulphonyl fluoride.	Phenylmethylsulfonyl Fluoride	193-224	keratin 9	Rattus norvegicus	13-16
7765287-5	1994	The WM-23 enzyme, inhibited by phenylmethylsulfonyl fluoride, may be classified to mammalian dipeptidyl aminopeptidase II.	Phenylmethylsulfonyl Fluoride	31-60	dipeptidyl peptidase 7	Homo sapiens	93-121