PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 33479914-2 2021 Our aim was to introduce hexahistidine (His6) peptides into pertuzumab Fab to enable labeling with the [99mTc(CO)3(H2O)3]+ complex and study these radioimmunoconjugates for microSPECT/CT imaging of HER2-positive tumor xenografts in mice. His-His-His-His-His-His 25-38 FA complementation group B Homo sapiens 71-74 33479914-2 2021 Our aim was to introduce hexahistidine (His6) peptides into pertuzumab Fab to enable labeling with the [99mTc(CO)3(H2O)3]+ complex and study these radioimmunoconjugates for microSPECT/CT imaging of HER2-positive tumor xenografts in mice. His-His-His-His-His-His 25-38 erb-b2 receptor tyrosine kinase 2 Mus musculus 198-202 33479914-2 2021 Our aim was to introduce hexahistidine (His6) peptides into pertuzumab Fab to enable labeling with the [99mTc(CO)3(H2O)3]+ complex and study these radioimmunoconjugates for microSPECT/CT imaging of HER2-positive tumor xenografts in mice. His-His-His-His-His-His 40-44 FA complementation group B Homo sapiens 71-74 33479914-2 2021 Our aim was to introduce hexahistidine (His6) peptides into pertuzumab Fab to enable labeling with the [99mTc(CO)3(H2O)3]+ complex and study these radioimmunoconjugates for microSPECT/CT imaging of HER2-positive tumor xenografts in mice. His-His-His-His-His-His 40-44 erb-b2 receptor tyrosine kinase 2 Mus musculus 198-202 33479914-3 2021 PROCEDURES: Fab were produced by papain digestion of pertuzumab and reacted with sulfo-SMCC for conjugation to His6-containing peptides (CGYGGHHHHHH). His-His-His-His-His-His 111-115 FA complementation group B Homo sapiens 12-15 33479914-10 2021 Fab were conjugated to 2.1 +- 0.5 His6 peptides and labeled with [99mTc(CO)3(H2O)3]+ to a radiochemical purity of 92-97 % at 0.4-0.8 MBq/mug. His-His-His-His-His-His 34-38 FA complementation group B Homo sapiens 0-3 31560800-3 2020 His achievements include discovering and cloning the elusive leukotriene E4 receptor (1), isolating and cloning the enzyme leukotriene C4 synthase (LTC4S) responsible for the first committed step in the production of the cysLTs (2), and the derivation of its crystal structure (3). His-His-His-His-His-His 0-3 leukotriene C4 synthase Homo sapiens 123-146 32929962-5 2020 Hereto, hexahistidine-tagged recombinant ADAMTS13 (rADAMTS13-His6) was immobilized on nitrilotriacetic acid (NTA)-coated FO probes chelated by cobalt (Co(III)) and exposed to anti-ADAMTS13 autoantibodies. His-His-His-His-His-His 8-21 ADAM metallopeptidase with thrombospondin type 1 motif 13 Homo sapiens 41-49 32929962-5 2020 Hereto, hexahistidine-tagged recombinant ADAMTS13 (rADAMTS13-His6) was immobilized on nitrilotriacetic acid (NTA)-coated FO probes chelated by cobalt (Co(III)) and exposed to anti-ADAMTS13 autoantibodies. His-His-His-His-His-His 8-21 ADAM metallopeptidase with thrombospondin type 1 motif, 13 Rattus norvegicus 51-60 32929962-5 2020 Hereto, hexahistidine-tagged recombinant ADAMTS13 (rADAMTS13-His6) was immobilized on nitrilotriacetic acid (NTA)-coated FO probes chelated by cobalt (Co(III)) and exposed to anti-ADAMTS13 autoantibodies. His-His-His-His-His-His 8-21 mitochondrially encoded cytochrome c oxidase III Homo sapiens 151-158 32929962-5 2020 Hereto, hexahistidine-tagged recombinant ADAMTS13 (rADAMTS13-His6) was immobilized on nitrilotriacetic acid (NTA)-coated FO probes chelated by cobalt (Co(III)) and exposed to anti-ADAMTS13 autoantibodies. His-His-His-His-His-His 8-21 ADAM metallopeptidase with thrombospondin type 1 motif 13 Homo sapiens 52-60 32334140-5 2020 Specifically, Nedd4 can be purified through a two-step purification which employs glutathione-S-transferase and hexahistidine sequences as orthogonal affinity tags. His-His-His-His-His-His 112-125 NEDD4 E3 ubiquitin protein ligase Homo sapiens 14-19 32384736-5 2020 The PA63-mediated delivery of His6-NDPK-A resulted in reduced migration of MDA-MB-231 cells, as determined by a wound-healing assay. His-His-His-His-His-His 30-34 NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens 35-41 31560800-3 2020 His achievements include discovering and cloning the elusive leukotriene E4 receptor (1), isolating and cloning the enzyme leukotriene C4 synthase (LTC4S) responsible for the first committed step in the production of the cysLTs (2), and the derivation of its crystal structure (3). His-His-His-His-His-His 0-3 leukotriene C4 synthase Homo sapiens 148-153 31988081-5 2020 Mutation of H82, the conserved metal-binding His residue in the HNH motif, to Ala abrogates gp74-mediated stimulation of terminase activity. His-His-His-His-His-His 45-48 Gp74 Escherichia phage HK97 92-96 31618135-4 2020 The present study aimed to produce recombinant TC-1 (rTC-1) with His tag to evaluate its antimicrobial activity and also predict its thermal-stability through molecular dynamic (MD) simulations. His-His-His-His-His-His 65-68 Serum cholesterol level QTL 22 Rattus norvegicus 47-51 31618135-4 2020 The present study aimed to produce recombinant TC-1 (rTC-1) with His tag to evaluate its antimicrobial activity and also predict its thermal-stability through molecular dynamic (MD) simulations. His-His-His-His-His-His 65-68 Serum cholesterol level QTL 22 Rattus norvegicus 53-58 31907281-10 2020 We further detected oxPL cross-link adducts of HDL apoproteins in plasma and aorta of hyperlipidemic LDLR-/- mice, including cross-link adducts of apoA-I His-165-apoA-I Lys-93, apoA-I His-154-apoA-I Lys-105, apoA-I His-154-apoA-IV Lys-149, and apoA-II Lys-30-apoE His-227. His-His-His-His-His-His 154-157 apolipoprotein A-I Mus musculus 147-153 31907281-10 2020 We further detected oxPL cross-link adducts of HDL apoproteins in plasma and aorta of hyperlipidemic LDLR-/- mice, including cross-link adducts of apoA-I His-165-apoA-I Lys-93, apoA-I His-154-apoA-I Lys-105, apoA-I His-154-apoA-IV Lys-149, and apoA-II Lys-30-apoE His-227. His-His-His-His-His-His 184-187 apolipoprotein A-I Mus musculus 147-153 31786226-5 2020 The enterokinase (EK) digestion was optimized for efficient cleavage of the Trx-His-S tag from each HSP90 construct by varying concentrations of EK (0.5-1 U) and urea (0-3 M). His-His-His-His-His-His 80-83 thioredoxin Homo sapiens 76-79 31783140-5 2020 Moreover, the concentrations of Thr, Cys, Leu, Lys, and His in the eggshell membranes were significantly higher (P < 0.05) in the P4-2 h treated hens whereas Val and Lys were significantly lower (P < 0.05) in P4-5 h treated hens compared to the control. His-His-His-His-His-His 56-59 glutamate-ammonia ligase Gallus gallus 133-137 31907281-10 2020 We further detected oxPL cross-link adducts of HDL apoproteins in plasma and aorta of hyperlipidemic LDLR-/- mice, including cross-link adducts of apoA-I His-165-apoA-I Lys-93, apoA-I His-154-apoA-I Lys-105, apoA-I His-154-apoA-IV Lys-149, and apoA-II Lys-30-apoE His-227. His-His-His-His-His-His 184-187 apolipoprotein A-I Mus musculus 147-153 31907281-10 2020 We further detected oxPL cross-link adducts of HDL apoproteins in plasma and aorta of hyperlipidemic LDLR-/- mice, including cross-link adducts of apoA-I His-165-apoA-I Lys-93, apoA-I His-154-apoA-I Lys-105, apoA-I His-154-apoA-IV Lys-149, and apoA-II Lys-30-apoE His-227. His-His-His-His-His-His 184-187 apolipoprotein A-I Mus musculus 147-153 31586598-5 2020 In the current study, we demonstrate an alternative route for producing active Ulp1 catalytic domain from a His-tagged N-terminally truncated variant, residues 416-621, which is expressed in E. coli inclusion bodies and subsequently refolded. His-His-His-His-His-His 108-111 SUMO protease ULP1 Saccharomyces cerevisiae S288C 79-83 32019204-7 2020 Molecular modelling method showed, that AEDG peptide preferably binds with H1/6 and H1/3 histones in His-Pro-Ser-Tyr-Met-Ala-His-Pro-Ala-Arg-Lys and Tyr-Arg-Lys-Thr-Gln sites, which interact with DNA. His-His-His-His-His-His 101-104 H1.6 linker histone, cluster member Homo sapiens 75-88 31760318-12 2020 In addition, P4-His, P6-Val, and P8-Pro in the peptide were exposed, and these residues as epitopes can be presented by SLA-2*04:02:02. His-His-His-His-His-His 13-19 src-like-adapter 2 Sus scrofa 120-125 31968674-4 2020 NTAN1 adopts the characteristic scaffold of CNF1/YfiH-like cysteine hydrolases that features an alpha-beta-beta sandwich structure and a catalytic triad comprising Cys, His, and Ser. His-His-His-His-His-His 169-172 N-terminal asparagine amidase Homo sapiens 0-5 32013233-5 2020 The results show that peptides IIAE, LIVTQ, and LVYPFP formed strong H bonds with the amino acids Gln 259, His 331, and Thr 358 in the active site of the human ACE. His-His-His-His-His-His 107-110 angiotensin I converting enzyme Homo sapiens 160-163 32013233-7 2020 Furthermore, peptides IIAE and LVYPFP interacted with the amino acid residues Gln 259 and His 331, respectively, also in common with other ACE-inhibitory drugs such as Captopril, Lisinopril and Elanapril. His-His-His-His-His-His 90-93 angiotensin I converting enzyme Homo sapiens 139-142 32015602-2 2020 His biopsy had features of PhosphoLipase A2 Receptor (PLA2R) positive membranous nephropathy with circumferential cellular crescents. His-His-His-His-His-His 0-3 phospholipase A2 receptor 1 Homo sapiens 27-52 32015602-2 2020 His biopsy had features of PhosphoLipase A2 Receptor (PLA2R) positive membranous nephropathy with circumferential cellular crescents. His-His-His-His-His-His 0-3 phospholipase A2 receptor 1 Homo sapiens 54-59 31706823-2 2021 METHODS: The sequence encoding the L-lectin module of SraP was inserted into pET28a plasmid, and the recombinant protein was purified by His label affinity chromatography. His-His-His-His-His-His 137-140 steroid receptor RNA activator 1 Mus musculus 54-58 31698900-4 2019 More interestingly, phosphate-containing metabolites can severely inhibit the peroxidase-like activity of His-AuNCs by blocking the generation of O2 - and electron transfer, and then ALP is able to restore the inhibition process through hydrolyzing the phosphate-containing metabolites. His-His-His-His-His-His 106-109 alkaline phosphatase, placental Homo sapiens 183-186 31145609-0 2019 Murine Calprotectin Coordinates Mn(II) at a Hexahistidine Site with Ca(II)-Dependent Affinity. His-His-His-His-His-His 44-57 carbonic anhydrase 2 Mus musculus 68-74 31653198-8 2019 We applied 7C to all CTCF motif pairs within 1 Mb in the human genome and validated predicted interactions with high-resolution Hi-C and ChIA-PET. His-His-His-His-His-His 128-132 CCCTC-binding factor Homo sapiens 21-25 31145609-6 2019 This site is a His6 site composed of His17 and His27 of mS100A8 and His92, His97, His105, and His107 of mS100A9. His-His-His-His-His-His 15-19 S100 calcium binding protein A8 (calgranulin A) Mus musculus 56-63 31145609-6 2019 This site is a His6 site composed of His17 and His27 of mS100A8 and His92, His97, His105, and His107 of mS100A9. His-His-His-His-His-His 15-19 S100 calcium binding protein A9 (calgranulin B) Mus musculus 104-111 31145609-8 2019 Mn(II) coordination to the His6 site also promotes self-association of two mCP heterodimers to form a heterotetramer. His-His-His-His-His-His 27-31 CD46 antigen, complement regulatory protein Mus musculus 75-78 31624759-9 2019 His CD4 count was 24 cells/muL. His-His-His-His-His-His 0-3 CD4 molecule Homo sapiens 4-7 31772756-5 2019 His serum IgE level was 12 040 U/mL (normal range 0-358). His-His-His-His-His-His 0-3 immunoglobulin heavy constant epsilon Homo sapiens 10-13 31736580-6 2019 His brain magnetic resonance imaging showed characteristic features of CTX, and the diagnosis was confirmed by demonstrating the mutation in exon 2 of the CYP27A1 gene. His-His-His-His-His-His 0-3 cytochrome P450 family 27 subfamily A member 1 Homo sapiens 155-162 31599264-6 2019 His serum Alpha-Feto Protein (AFP) level was very high with elevated serum alanine amino transaminase (ALT) enzyme and prothrombin time. His-His-His-His-His-His 0-3 alpha fetoprotein Homo sapiens 10-28 31599264-6 2019 His serum Alpha-Feto Protein (AFP) level was very high with elevated serum alanine amino transaminase (ALT) enzyme and prothrombin time. His-His-His-His-His-His 0-3 alpha fetoprotein Homo sapiens 30-33 31599264-6 2019 His serum Alpha-Feto Protein (AFP) level was very high with elevated serum alanine amino transaminase (ALT) enzyme and prothrombin time. His-His-His-His-His-His 0-3 coagulation factor II, thrombin Homo sapiens 119-130 31624517-9 2019 The genotype distribution for ADH1B was not significantly different between symptomatic and asymptomatic groups, but the genotype ADH1B His/His was significantly more prevalent in the alcohol-related group than in the alcohol-unrelated group (81.3% vs 50%, P = .023). His-His-His-His-His-His 136-139 alcohol dehydrogenase 1B (class I), beta polypeptide Homo sapiens 130-135 31624517-9 2019 The genotype distribution for ADH1B was not significantly different between symptomatic and asymptomatic groups, but the genotype ADH1B His/His was significantly more prevalent in the alcohol-related group than in the alcohol-unrelated group (81.3% vs 50%, P = .023). His-His-His-His-His-His 140-143 alcohol dehydrogenase 1B (class I), beta polypeptide Homo sapiens 130-135 31624517-10 2019 In multivariate logistic regression analysis, the genotype of ADH1B His/His was independently associated with syncope after alcohol drinking (odds ratio, 5.746; 95% confidence interval, 1.580-28.421; P = .007). His-His-His-His-His-His 68-71 alcohol dehydrogenase 1B (class I), beta polypeptide Homo sapiens 62-67 31624517-10 2019 In multivariate logistic regression analysis, the genotype of ADH1B His/His was independently associated with syncope after alcohol drinking (odds ratio, 5.746; 95% confidence interval, 1.580-28.421; P = .007). His-His-His-His-His-His 72-75 alcohol dehydrogenase 1B (class I), beta polypeptide Homo sapiens 62-67 31263161-0 2019 His6, His13, and His14 residues in Abeta 1-40 peptide significantly and specifically affect oligomeric equilibria. His-His-His-His-His-His 0-4 amyloid beta precursor protein Homo sapiens 35-40 31543773-7 2019 His serum PSA level had gradually decreased, but dysgeusia appeared, so we changed the timing of enzalutamide intake from morning to night just before sleep without dose reduction. His-His-His-His-His-His 0-3 aminopeptidase puromycin sensitive Homo sapiens 10-13 31242384-0 2019 MicroSPECT/CT Imaging of Cell-Line and Patient-Derived EGFR-Positive Tumor Xenografts in Mice with Panitumumab Fab Modified with Hexahistidine Peptides To Enable Labeling with 99mTc(I) Tricarbonyl Complex. His-His-His-His-His-His 129-142 epidermal growth factor receptor Homo sapiens 55-59 30240633-5 2019 Here, we report the co-expression of CTK2-CTK3 complex from Saccharomyces cerevisiae with N-terminal His6-tag in CTK3 in Escherichia coli (E. coli), purification of the complex by four chromatographic steps and crystallization of the complex as well as the diffraction data collection and processing. His-His-His-His-His-His 101-105 Ctk2p Saccharomyces cerevisiae S288C 37-41 31204687-7 2019 Moreover, the C-terminal His6 tag of the recombinant protein binds to a shallow pocket adjacent to the iNOS-binding site on a crystallographically related SPSB2 molecule. His-His-His-His-His-His 25-29 nitric oxide synthase 2 Homo sapiens 103-107 31204687-7 2019 Moreover, the C-terminal His6 tag of the recombinant protein binds to a shallow pocket adjacent to the iNOS-binding site on a crystallographically related SPSB2 molecule. His-His-His-His-His-His 25-29 splA/ryanodine receptor domain and SOCS box containing 2 Homo sapiens 155-160 30605338-2 2019 We synthesized a 26-mer peptide-YSEILFFVQS-HHHHHH-LAMGWTNMLY (S3HS4)-comprising two chemoreceptor domains of transient receptor potential channel (TRPV1) linked by a hexahistidine sequence. His-His-His-His-His-His 166-179 transient receptor potential cation channel subfamily V member 1 Homo sapiens 147-152 31089613-3 2019 A hexahistidine-tag on MDM2 allows the binding of the protein complex to the Ni-NTA affinity resin, while the fluorescent protein fused to p53 enables the direct visualization of the interaction of p53 with MDM2. His-His-His-His-His-His 2-15 MDM2 proto-oncogene Homo sapiens 23-27 30998775-6 2019 To examine the in vivo effect of MBP-fused, truncated flatfish MSTNpro, MBP-Pro45-70-His6 (20 mg/kg body wt) was subcutaneously injected 5 times for 14 days in mice. His-His-His-His-His-His 85-89 myelin basic protein Mus musculus 72-75 30998775-10 2019 The presence of antibody against MBP-Pro45-70-His6 was observed at both 7 and 14 d after the administration with the titer value at 14 d being much greater than that at 7 d, suggesting that antibodies against MBP-Pro45-70-His6 neutralized the MSTN-inhibitory effect of MBP-Pro45-70-His6. His-His-His-His-His-His 46-50 myelin basic protein Mus musculus 33-36 30998775-10 2019 The presence of antibody against MBP-Pro45-70-His6 was observed at both 7 and 14 d after the administration with the titer value at 14 d being much greater than that at 7 d, suggesting that antibodies against MBP-Pro45-70-His6 neutralized the MSTN-inhibitory effect of MBP-Pro45-70-His6. His-His-His-His-His-His 46-50 myelin basic protein Mus musculus 209-212 30998775-10 2019 The presence of antibody against MBP-Pro45-70-His6 was observed at both 7 and 14 d after the administration with the titer value at 14 d being much greater than that at 7 d, suggesting that antibodies against MBP-Pro45-70-His6 neutralized the MSTN-inhibitory effect of MBP-Pro45-70-His6. His-His-His-His-His-His 46-50 myostatin Mus musculus 243-247 30998775-10 2019 The presence of antibody against MBP-Pro45-70-His6 was observed at both 7 and 14 d after the administration with the titer value at 14 d being much greater than that at 7 d, suggesting that antibodies against MBP-Pro45-70-His6 neutralized the MSTN-inhibitory effect of MBP-Pro45-70-His6. His-His-His-His-His-His 46-50 myelin basic protein Mus musculus 209-212 30998775-10 2019 The presence of antibody against MBP-Pro45-70-His6 was observed at both 7 and 14 d after the administration with the titer value at 14 d being much greater than that at 7 d, suggesting that antibodies against MBP-Pro45-70-His6 neutralized the MSTN-inhibitory effect of MBP-Pro45-70-His6. His-His-His-His-His-His 222-226 myelin basic protein Mus musculus 33-36 30998775-10 2019 The presence of antibody against MBP-Pro45-70-His6 was observed at both 7 and 14 d after the administration with the titer value at 14 d being much greater than that at 7 d, suggesting that antibodies against MBP-Pro45-70-His6 neutralized the MSTN-inhibitory effect of MBP-Pro45-70-His6. His-His-His-His-His-His 222-226 myelin basic protein Mus musculus 209-212 30998775-10 2019 The presence of antibody against MBP-Pro45-70-His6 was observed at both 7 and 14 d after the administration with the titer value at 14 d being much greater than that at 7 d, suggesting that antibodies against MBP-Pro45-70-His6 neutralized the MSTN-inhibitory effect of MBP-Pro45-70-His6. His-His-His-His-His-His 222-226 myostatin Mus musculus 243-247 30998775-10 2019 The presence of antibody against MBP-Pro45-70-His6 was observed at both 7 and 14 d after the administration with the titer value at 14 d being much greater than that at 7 d, suggesting that antibodies against MBP-Pro45-70-His6 neutralized the MSTN-inhibitory effect of MBP-Pro45-70-His6. His-His-His-His-His-His 222-226 myelin basic protein Mus musculus 209-212 30240633-5 2019 Here, we report the co-expression of CTK2-CTK3 complex from Saccharomyces cerevisiae with N-terminal His6-tag in CTK3 in Escherichia coli (E. coli), purification of the complex by four chromatographic steps and crystallization of the complex as well as the diffraction data collection and processing. His-His-His-His-His-His 101-105 Ctk3p Saccharomyces cerevisiae S288C 42-46 30240633-5 2019 Here, we report the co-expression of CTK2-CTK3 complex from Saccharomyces cerevisiae with N-terminal His6-tag in CTK3 in Escherichia coli (E. coli), purification of the complex by four chromatographic steps and crystallization of the complex as well as the diffraction data collection and processing. His-His-His-His-His-His 101-105 Ctk3p Saccharomyces cerevisiae S288C 113-117 30010769-6 2018 In vitro crosslink analysis was performed using a hexahistidine-tagged calmodulin and glutathione S-transferase-fused forms of VIP1 and its close homologues. His-His-His-His-His-His 50-63 VIRE2-interacting protein 1 Arabidopsis thaliana 127-131 29691140-4 2018 By using amine reactive fluorescent N-hydroxysuccinimidyl (NHS)-ester dyes and by direct detection with primary fluorescently conjugated anti-histidine (His-tag) antibodies against detect N-terminal His6, we show Eap subdomain Eap D3D4 specifically interacts and rapidly activates human platelets. His-His-His-His-His-His 199-203 glutamyl aminopeptidase Homo sapiens 213-216 30352511-5 2018 Two cysteines were judiciously incorporated at the beginning of the scFv hexahistidine tag, in order to allow controlled bioconjugation of a heterobifunctional linker including a second generation maleimide (SGM), either cleavable (for monomethyl auristatin E) or noncleavable (for monomethyl auristatin F). His-His-His-His-His-His 73-86 immunglobulin heavy chain variable region Homo sapiens 68-72 29691140-4 2018 By using amine reactive fluorescent N-hydroxysuccinimidyl (NHS)-ester dyes and by direct detection with primary fluorescently conjugated anti-histidine (His-tag) antibodies against detect N-terminal His6, we show Eap subdomain Eap D3D4 specifically interacts and rapidly activates human platelets. His-His-His-His-His-His 199-203 glutamyl aminopeptidase Homo sapiens 227-230 29691140-5 2018 Furthermore, we validate our finding by using site directed directional immobilization of Eap D3D4 through N-terminal His6 on nickel (II)-nitrilotriacetic acid (Ni-NTA) functionalized bacteriomimetic microbead arrays to visualize real-time platelet activation through calcium release assay. His-His-His-His-His-His 118-122 glutamyl aminopeptidase Homo sapiens 90-93 29717998-3 2018 Recombinant AKR1C3 with a thrombin-cleavable N-terminal His6 tag was expressed from a pET-28(+) vector for structural studies of enzyme-inhibitor complexes. His-His-His-His-His-His 56-60 aldo-keto reductase family 1 member C3 Homo sapiens 12-18 29890074-12 2018 In contrast, negligible differences are observed for the Ni(II)-His6 site in the absence and presence of Ca(II). His-His-His-His-His-His 64-68 carbonic anhydrase 2 Homo sapiens 105-111 29553717-4 2018 We developed two novel bioengineered scFv carrying two cysteines located (i) at the end (4D5.1-cys2) or (ii) at the beginning (4D5.2-cys2) of its hexahistidine tag. His-His-His-His-His-His 146-159 immunglobulin heavy chain variable region Homo sapiens 37-41 29553717-6 2018 Our data indicated that the insertion of cysteines at the beginning of the hexahistidine tag was allowed to obtain nearly 2-fold conjugation efficiency (13 scFv/NP) compared to NMs using classical maleimide. His-His-His-His-His-His 75-88 immunglobulin heavy chain variable region Homo sapiens 156-160 29717998-3 2018 Recombinant AKR1C3 with a thrombin-cleavable N-terminal His6 tag was expressed from a pET-28(+) vector for structural studies of enzyme-inhibitor complexes. His-His-His-His-His-His 56-60 coagulation factor II, thrombin Homo sapiens 26-34 29118030-3 2018 Here, we present a robust Tn5 transposase purification strategy based on an N-terminal His6-Sumo3 tag. His-His-His-His-His-His 87-91 small ubiquitin like modifier 3 Homo sapiens 92-97 29391000-13 2018 Moreover, molecular docking study of BA with PLA2 revealed that BA interacts with GLY22 & GLY29 through hydrogen bond formation and LEU2, PHE5, HIS6, ALA17, ALA18, HIS47 and TYR51 through different types of hydrophobic interactions. His-His-His-His-His-His 148-152 phospholipase A2 group IB Homo sapiens 45-49 29338205-2 2018 Coupling of a peptide chelator, hexahistidine, with hydrophobic oligostyrene allows a modular strategy to be established for the efficient synthesis and purification of these tunable amphiphiles (oSt(His)6). His-His-His-His-His-His 32-45 dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit Homo sapiens 196-199 29456767-6 2018 Methods: We constructed two variants of hexahistidine-tagged human recombinant fumarase, A308T and H318Y, associated with FHD and HLRCC, respectively. His-His-His-His-His-His 40-53 fumarate hydratase Homo sapiens 79-87 31938131-12 2018 His CD4-positive T-lymphocyte counts were low, and oligoclonal CD8-positive alphabeta T-lymphocytes were present in the blood prior to the first and after three cycles of chemotherapy. His-His-His-His-His-His 0-3 CD4 molecule Homo sapiens 4-7 31938131-16 2018 His immunological characteristics, including low CD4-positive or CD8-positive alphabeta T-lymphocytes, may have contributed to the unusual clinical presentations by N. otitidiscaviarum, which rarely involves the central nervous system. His-His-His-His-His-His 0-3 CD4 molecule Homo sapiens 49-52 31938131-16 2018 His immunological characteristics, including low CD4-positive or CD8-positive alphabeta T-lymphocytes, may have contributed to the unusual clinical presentations by N. otitidiscaviarum, which rarely involves the central nervous system. His-His-His-His-His-His 0-3 CD8a molecule Homo sapiens 65-68 31631089-2 2018 His serum PSA level was 29.02 ng/ml. His-His-His-His-His-His 0-3 aminopeptidase puromycin sensitive Homo sapiens 10-13 30196503-2 2018 Detailed protocols are provided for transient mammalian expression in a hexahistidine-tagged Fab format, purification by immobilized metal affinity chromatography (IMAC), and affinity determination by BioLayer interferometry (BLI). His-His-His-His-His-His 72-85 FA complementation group B Homo sapiens 93-96 28893510-7 2017 The complex between GST-Gpi823-306 (alpha) and His6-Gaa150-343 (beta) was characterized by native gel analysis and size exclusion chromatography (SEC) and results are most consistent with an alpha2beta2 stoichiometry. His-His-His-His-His-His 47-51 GPI-anchor transamidase Saccharomyces cerevisiae S288C 24-34 28800637-2 2017 The human EPO construct was amplified with C-terminal hexahistidine tag from a cDNA of Caco-2 cells. His-His-His-His-His-His 54-67 erythropoietin Homo sapiens 10-13 29085768-7 2017 Due to these interactions, the large polycyclic moiety of the ligand would also block further interactions with Hsd6 (prototropic tautomer of His), Asp7, Ser8 and Gly9 that are integral to His6-His13-His14, Arg5-Asp7and Leu34-Gly38 beta-sheets, salt bridges in Glu22-Lys28 and turn conformation Phe19-Gly25. His-His-His-His-His-His 189-193 RNA, U1 small nuclear 4 Homo sapiens 112-116 28545467-7 2017 RESULTS: In this paper we report, for the first time, the expression in a baculovirus system of a deleted recombinant C-terminal 6xHis-tagged atypical CPB2 toxin (rCPB2Delta1-25-His6) lacking the 25 amino acids (aa) of the N-terminal putative signal sequence. His-His-His-His-His-His 178-182 carboxypeptidase B2 Homo sapiens 151-155 28319815-4 2017 Bivalent cobalt ions were coupled to the carboxyls on the surface of the obtained Co3O4 NHs so as to chelate the hexahistidine residues (His-Tags) at the C-terminal of EGFR single-domain antibodies (EGFR sdAbs). His-His-His-His-His-His 113-126 epidermal growth factor receptor Homo sapiens 168-172 26739785-6 2016 An N-terminal Glutathione-S-Transferase tag was used to promote soluble expression and was combined with a C-terminal hexahistidine tag to facilitate a simple non-denaturing two-step affinity chromatography procedure that results in highly pure full-length H1.4. His-His-His-His-His-His 118-131 H1.4 linker histone, cluster member Homo sapiens 257-261 28685146-2 2017 To facilitate recombinant proteins purification, detection, and stability the baculovirus expression vectors were constructed to bear N-terminal His6-FLAG tag. His-His-His-His-His-His 145-149 long intergenic non-protein coding RNA 1194 Homo sapiens 155-158 27541598-4 2016 In this study, we report that CP binds Zn(II) at this site using a hexahistidine motif, completed by His103 and His105 of the S100A9 C-terminal tail and previously identified as the high-affinity Mn(II) and Fe(II) coordination site. His-His-His-His-His-His 67-80 S100 calcium binding protein A9 Homo sapiens 126-132 27414784-5 2016 The recombinant IL-38 containing a C-hexahistidine tag was expressed in Escherichia coli BL21 (DE3) which induced by isopropyl-beta-D-thiogalactoside. His-His-His-His-His-His 37-50 interleukin 1 family member 10 Homo sapiens 16-21 27622309-3 2016 We used the Zn outer shell of the QD to bind HIS6 in JB577 (W G Dap(N-Palmitoyl) VKIKK P9 G2 H6 ) and by a gel-shift assay showed that siRNAs would bind to the positively charged KIKK sequence. His-His-His-His-His-His 45-49 death associated protein Homo sapiens 64-67 26111765-6 2015 In addition to the N-substituted pyrrole of [N-C4H2]-Ang II and Michael addition products of [His(6)(EDE)]-Ang II, hydrated forms were detected as major products, suggesting considerable involvement of the vicinal dihydrodiol (formed by epoxide hydration) in EDE-derived protein modification in vivo. His-His-His-His-His-His 94-100 angiogenin Homo sapiens 107-110 32025526-6 2016 His parathyroid hormone (PTH) level was 106 pmol/L, corrected calcium level was 2.29 mmol/L and phosphate 1.63 mmol/L under maximal medical treatment, and he had significant bone disease. His-His-His-His-His-His 0-3 parathyroid hormone Homo sapiens 4-23 32025526-6 2016 His parathyroid hormone (PTH) level was 106 pmol/L, corrected calcium level was 2.29 mmol/L and phosphate 1.63 mmol/L under maximal medical treatment, and he had significant bone disease. His-His-His-His-His-His 0-3 parathyroid hormone Homo sapiens 25-28 27123472-2 2016 METHODS: His6-N-hCPT2 (wild-type) and His6-N-hCPT2/S113L (variant) were produced recombinantly in prokaryotic host and characterized according to their functional and regulatory properties. His-His-His-His-His-His 9-13 carnitine palmitoyltransferase 2 Homo sapiens 16-21 26492302-2 2015 In the present study, A8-35, a polyacrylate-based APol, was grafted with hexahistidine tags (His6-tags). His-His-His-His-His-His 73-86 apolipoprotein L1 Homo sapiens 50-54 26492302-5 2015 Compared to a single His6-tag fused at one extremity of a MP, the presence of several His6-tags carried by the APol belt surrounding the transmembrane domain of a MP increases remarkably the affinity of the protein/APol complex for nickel ion-bearing SPR chips, whereas it does not show such a strong effect on an IMAC resin. His-His-His-His-His-His 21-25 apolipoprotein L1 Homo sapiens 111-115 26492302-5 2015 Compared to a single His6-tag fused at one extremity of a MP, the presence of several His6-tags carried by the APol belt surrounding the transmembrane domain of a MP increases remarkably the affinity of the protein/APol complex for nickel ion-bearing SPR chips, whereas it does not show such a strong effect on an IMAC resin. His-His-His-His-His-His 21-25 apolipoprotein L1 Homo sapiens 215-219 26510597-2 2015 Two genetic constructs encoding full-length serpin B3 and serpin B4 molecules were created to produce recombinant SPB3 and SPB4 proteins carrying a N-terminal His6-tag. His-His-His-His-His-His 159-163 serpin family B member 4 Homo sapiens 58-67 25372819-4 2014 In this work, B. licheniformis ATCC 14580 allantoinase (AllBali) containing a C-terminal His6 tag was overproduced in Escherichia coli and purified to homogeneity. His-His-His-His-His-His 89-93 allantoinase AllB Bacillus licheniformis DSM 13 = ATCC 14580 42-54 26174665-4 2015 The Rep protein was expressed as a fusion with the hexa-histidine (His) at its C-terminal end and purified by Ni-affinity chromatography. His-His-His-His-His-His 51-65 Rep Lactobacillus plantarum 4-7 26174665-4 2015 The Rep protein was expressed as a fusion with the hexa-histidine (His) at its C-terminal end and purified by Ni-affinity chromatography. His-His-His-His-His-His 67-70 Rep Lactobacillus plantarum 4-7 25756537-4 2015 IFN-lambda3 contains a hexahistidine tag at its C-terminus. His-His-His-His-His-His 23-36 interferon lambda 3 Mus musculus 0-11 25551420-5 2015 BL21(DE3) Escherichia coli strain transformed with the rpb5 expression vector pET28a(+)-rpb5 accumulates large amounts of a soluble protein of about 30 kDa (25 kDa plus 5 kDa double His6-Tag at N and C-terminal). His-His-His-His-His-His 182-186 DNA-directed RNA polymerase core subunit RPB5 Saccharomyces cerevisiae S288C 88-92 25597447-2 2015 Human CP uses Ca(II) ions to tune its Mn(II) affinity at a biologically unprecedented hexahistidine site that forms at the S100A8/S100A9 interface, and the molecular basis for this phenomenon requires elucidation. His-His-His-His-His-His 86-99 carbonic anhydrase 2 Homo sapiens 14-20 25597447-5 2015 The CW EPR spectrum of Ca(II)- and Mn(II)-bound CP prepared with a 10:0.9:1 Ca(II):Mn(II):CP ratio is characterized by an unusually low zero-field splitting of 485 MHz (E/D = 0.30) for the S = 5/2 Mn(II) ion, consistent with the high symmetry of the His6 binding site observed crystallographically. His-His-His-His-His-His 250-254 carbonic anhydrase 2 Homo sapiens 23-29 25597447-9 2015 This study demonstrates the role of Ca(II) in enabling high-affinity and specific binding of Mn(II) to the His6 site of human calprotectin. His-His-His-His-His-His 107-111 carbonic anhydrase 2 Homo sapiens 36-42 24863735-2 2015 We previously produced hexahistidine-tagged, bovine and porcine FGF4 (Pro(32) to Leu(206) ) proteins without a secretory signal peptide at the aminoterminus in Escherichia coli. His-His-His-His-His-His 23-36 fibroblast growth factor 4 Bos taurus 64-68 25551420-5 2015 BL21(DE3) Escherichia coli strain transformed with the rpb5 expression vector pET28a(+)-rpb5 accumulates large amounts of a soluble protein of about 30 kDa (25 kDa plus 5 kDa double His6-Tag at N and C-terminal). His-His-His-His-His-His 182-186 DNA-directed RNA polymerase core subunit RPB5 Saccharomyces cerevisiae S288C 55-59 25229859-1 2014 Human macrophage dectin-1, a type II transmembrane beta-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for binding activity. His-His-His-His-His-His 126-139 C-type lectin domain containing 7A Homo sapiens 17-25 24998777-0 2014 Purification and characterization of bioactive his6-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells. His-His-His-His-His-His 47-51 TIMP metallopeptidase inhibitor 1 Homo sapiens 77-117 24998777-0 2014 Purification and characterization of bioactive his6-tagged recombinant human tissue inhibitor of metalloproteinases-1 (TIMP-1) protein expressed at high yields in mammalian cells. His-His-His-His-His-His 47-51 TIMP metallopeptidase inhibitor 1 Homo sapiens 119-125 24269761-4 2014 In this study, a cDNA fragment (mCH) encoding the 25 amino acid mature peptide was cloned from channel catfish liver, and inserted into vector pET-32a(+) to produce a construct that expressed a hexahistidine-tagged thioredoxin (trxA) fusion protein that was cleavable using enterokinase. His-His-His-His-His-His 194-207 modifier of chinchilla Mus musculus 32-35 24269761-4 2014 In this study, a cDNA fragment (mCH) encoding the 25 amino acid mature peptide was cloned from channel catfish liver, and inserted into vector pET-32a(+) to produce a construct that expressed a hexahistidine-tagged thioredoxin (trxA) fusion protein that was cleavable using enterokinase. His-His-His-His-His-His 194-207 thioredoxin Ictalurus punctatus 215-226 24866129-2 2014 Using hexahistidine tagged, recombinant Lot6p, we determined the steady-state enzyme kinetic parameters with both NADH and NADPH as electron donors; no cooperativity was observed with these substrates. His-His-His-His-His-His 6-19 flavin-dependent quinone reductase Saccharomyces cerevisiae S288C 40-45 24005034-3 2013 The immobilization of His6-RAGE domains consists of: (i) formation of a mixed layer of N-acetylcysteamine (NAC) and the thiol derivative of pentetic acid (DPTA); (ii) complexation of Cu(II) by DPTA; (iii) oriented immobilization of His6-RAGE domains via coordination bonds between Cu(II) sites from DPTA-Cu(II) complex and imidazole nitrogen atoms of a histidine tag. His-His-His-His-His-His 22-26 advanced glycosylation end-product specific receptor Homo sapiens 27-31 23951570-5 2013 The membrane fractions containing the expressed T2R4 were extracted and immobilized on the gold surface of a quartz crystal microbalance (QCM) pretreated with a monolayer of self-assembled aptamers that can specifically recognize and capture biomolecules labeled with His6-tags. His-His-His-His-His-His 268-272 taste 2 receptor member 4 Homo sapiens 48-52 23179057-3 2013 Here we report NMR chemical shift assignment of recombinant human BASP1 fused to an N-terminal cleavable His6-tag. His-His-His-His-His-His 105-109 brain abundant membrane attached signal protein 1 Homo sapiens 66-71 24005034-3 2013 The immobilization of His6-RAGE domains consists of: (i) formation of a mixed layer of N-acetylcysteamine (NAC) and the thiol derivative of pentetic acid (DPTA); (ii) complexation of Cu(II) by DPTA; (iii) oriented immobilization of His6-RAGE domains via coordination bonds between Cu(II) sites from DPTA-Cu(II) complex and imidazole nitrogen atoms of a histidine tag. His-His-His-His-His-His 22-26 advanced glycosylation end-product specific receptor Homo sapiens 237-241 23274363-3 2013 Membranes functionalized with nitrilotriacetate and glutathione captured and released hexahistidine-tagged enhanced green fluorescent protein (His-tag GFP) and glutathione S-transferase (GST) selectively under appropriate conditions. His-His-His-His-His-His 86-99 glutathione S-transferase kappa 1 Homo sapiens 187-190 23393142-4 2013 To address these questions, we developed a system to mimic the membrane anchoring of Rho GTPases by creating liposomes containing both PIP2 and a Ni(2+)-NTA modified lipid capable of binding hexahistidine-tagged Cdc42. His-His-His-His-His-His 191-204 cell division cycle 42 Homo sapiens 212-217 23424095-7 2013 The bacterial cell-free method used pET 100/D-TOPO vector to synthesize hexa-histidine-tagged D2L receptor using a dialysis bag system; the resulting protein was purified using nickel-nitrilotriacetic acid affinity resin. His-His-His-His-His-His 72-86 PET100 cytochrome c oxidase chaperone Homo sapiens 36-43 23924466-7 2013 RESULTS: TPO ectodomain was recovered from the culture media as a soluble protein, and it was fused with a hexahistidine tag which allowed purification by nickel-affinity chromatography. His-His-His-His-His-His 107-120 thyroid peroxidase Homo sapiens 9-12 23832333-2 2013 In this study the expression and purification of a hexahistidine-tagged mouse Aos1-Uba2-fusion protein (His6-mAU) in a baculovirus-insect cell system revealed that approximately 1.0 mg of highly-purified His6-mAU was obtained from 100 mL of Sf9 cell culture. His-His-His-His-His-His 51-64 SUMO1 activating enzyme subunit 1 Mus musculus 78-82 23832333-2 2013 In this study the expression and purification of a hexahistidine-tagged mouse Aos1-Uba2-fusion protein (His6-mAU) in a baculovirus-insect cell system revealed that approximately 1.0 mg of highly-purified His6-mAU was obtained from 100 mL of Sf9 cell culture. His-His-His-His-His-His 51-64 ubiquitin-like modifier activating enzyme 2 Mus musculus 83-87 23069765-2 2013 Its polypeptide monomer contains one canonical N-glycosylation site at Asn68, and human recombinant IL-17A was partly N-glycosylated when expressed in human kidney (HEK293) cells as a fusion protein with a melittin signal sequence and an N-terminal hexahistidine tag. His-His-His-His-His-His 249-262 interleukin 17A Homo sapiens 100-106 22796659-3 2012 We have performed QM/MM calculations to study the mouse renin catalytic mechanism in atomistic detail, using the N-terminal His6-Asn14 segment of angiotensinogen as substrate. His-His-His-His-His-His 124-128 angiotensinogen (serpin peptidase inhibitor, clade A, member 8) Mus musculus 146-161 22696779-13 2004 Nanobody 7D12 was labeled with (99m)Tc at its C-terminus hexahistidine tail as a SPECT agent for imaging EGFR expression in tumors in mice (22). His-His-His-His-His-His 57-70 epidermal growth factor receptor Mus musculus 105-109 22809242-4 2012 Finally, we prepared a fluorescent probe utilizing the ligand with lowest off-rate and succeeded in performing single molecule observations of hexahistidine-tagged myosin V walking on actin filaments. His-His-His-His-His-His 143-156 myosin VA Homo sapiens 164-172 22696780-13 2004 Nanobody 7D12 was labeled with (99m)Tc at its C-terminus hexahistidine tail as a SPECT agent for imaging EGFR expression in tumors in mice (22). His-His-His-His-His-His 57-70 epidermal growth factor receptor Mus musculus 105-109 22171920-8 2011 The non-tagged scFv-Fc and hexahistidine-tagged scFv-Fc proteins were found predominantly as insoluble aggregates and therefore were not suitable for further purification and activity testing. His-His-His-His-His-His 27-40 immunglobulin heavy chain variable region Homo sapiens 48-52 22315216-10 2012 Non-conserved residues in the binding pocket that strongly contribute to D1/D2 receptor agonist selectivity were also identified; those were Ser/Cys3 36, Tyr/Phe5 38, Ser/Tyr5 41, and Asn/His6 55 in the transmembrane (TM) helix region, together with Ser/Ile and Leu/Asn in the second extracellular loop (EC2). His-His-His-His-His-His 188-192 leiomodin 1 Homo sapiens 73-78 21900421-5 2012 UL98 with an amino-terminal hexahistidine tag was expressed in Escherichia coli, purified by affinity chromatography and confirmed to have exo and endo activities. His-His-His-His-His-His 28-41 deoxyribonuclease Human betaherpesvirus 5 0-4 20842634-8 2011 The antigen-binding site is blocked by the hexahistidine tag of another Fab molecule in both crystal forms. His-His-His-His-His-His 43-56 FA complementation group B Homo sapiens 72-75 21371416-4 2011 A hexahistidine tag was genetically attached to the C terminus of the GST, and the 5" end of an oligonucleotide was conjugated with nitrilotriacetic acid (NTA) for the site-specific conjugation of the DNA with the GST based on a Ni(2+) complex interaction. His-His-His-His-His-His 2-15 glutathione S-transferase kappa 1 Homo sapiens 70-73 20851184-5 2011 The expressed beta-actin (hexahistidine-tagged) was separated with a Ni-chelating resin, followed by a polymerization/depolymerization cycle or column chromatography with the Ni-chelating resin. His-His-His-His-His-His 26-39 POTE ankyrin domain family member F Homo sapiens 14-24 21539457-4 2011 The H-protein cDNA was subsequently cloned with a hexahistidine affinity tag in the Pichia pastoris plasmid vector pPICZalphaA and recombined into the yeast genome downstream of the alcohol oxidase promoter for methanol-induced expression. His-His-His-His-His-His 50-63 myosin binding protein H Homo sapiens 4-13 21217145-4 2011 The previously crystallized hexa-histidine-specific 3D5 scFv antibody was modified in the complementary determining region and by random mutagenesis, in conjunction with phage display, to yield scFvs with new biochemical characteristics and binding specificity. His-His-His-His-His-His 28-42 immunglobulin heavy chain variable region Homo sapiens 56-60 21219946-4 2011 The hLIF cDNA was cloned into two different vectors in order to produce N-terminal His6-tag and Trx-His6-tag hLIF fusion proteins in Origami(DE3) Escherichia coli. His-His-His-His-His-His 83-87 LIF interleukin 6 family cytokine Homo sapiens 4-8 20870456-1 2011 Silkworm soluble trehalase was expressed as a fusion protein with an N-terminal or C-terminal hexahistidine tag in a baculovirus-silkworm expression system and assayed for enzymatic activity. His-His-His-His-His-His 94-107 trehalase Bombyx mori 17-26 21290629-0 2004 [(99m)Tc(CO)3](+)-Labeled anti-epidermal growth factor receptor (HER2) affibody ZHER2:342 with a hexa-histidine tag (H6) on the N-terminal Overexpression of the epidermal growth factor receptor type 2 (HER2) is a characteristic feature of a variety of cancers, and HER2 levels in tumors (primary or metastatic) are often used to screen for patients who would benefit from anti-HER2 antibody (Ab) therapy (e.g., for breast cancer), to determine the efficacy of a treatment regimen, or to predict the prognostic outcome for a patient (1). His-His-His-His-His-His 97-111 epidermal growth factor receptor Homo sapiens 31-63 21290629-0 2004 [(99m)Tc(CO)3](+)-Labeled anti-epidermal growth factor receptor (HER2) affibody ZHER2:342 with a hexa-histidine tag (H6) on the N-terminal Overexpression of the epidermal growth factor receptor type 2 (HER2) is a characteristic feature of a variety of cancers, and HER2 levels in tumors (primary or metastatic) are often used to screen for patients who would benefit from anti-HER2 antibody (Ab) therapy (e.g., for breast cancer), to determine the efficacy of a treatment regimen, or to predict the prognostic outcome for a patient (1). His-His-His-His-His-His 97-111 erb-b2 receptor tyrosine kinase 2 Homo sapiens 65-69 21290634-0 2004 [(99m)Tc(CO)3](+)-Labeled anti-epidermal growth factor receptor (HER2) affibody ZHER2:342 with a hexa-histidine tag (H6) on the C-terminal Overexpression of the epidermal growth factor receptor type 2 (HER2) is a characteristic feature of a variety of cancers, and HER2 levels in tumors (primary or metastatic) are often used to screen for patients who would benefit from anti-HER2 antibody (Ab) therapy (e.g., for breast cancer), to determine the efficacy of a treatment regimen, or to predict the prognostic outcome for a patient (1). His-His-His-His-His-His 97-111 epidermal growth factor receptor Homo sapiens 31-63 21290634-0 2004 [(99m)Tc(CO)3](+)-Labeled anti-epidermal growth factor receptor (HER2) affibody ZHER2:342 with a hexa-histidine tag (H6) on the C-terminal Overexpression of the epidermal growth factor receptor type 2 (HER2) is a characteristic feature of a variety of cancers, and HER2 levels in tumors (primary or metastatic) are often used to screen for patients who would benefit from anti-HER2 antibody (Ab) therapy (e.g., for breast cancer), to determine the efficacy of a treatment regimen, or to predict the prognostic outcome for a patient (1). His-His-His-His-His-His 97-111 erb-b2 receptor tyrosine kinase 2 Homo sapiens 65-69 22191059-2 2011 The results indicate that the N-terminal Asp binds to Cu(II) together with His6 and either His13 or His14 to form the lower pH Component I of Abeta. His-His-His-His-His-His 75-79 amyloid beta precursor protein Homo sapiens 142-147 22191059-3 2011 Component II of Abeta is the complex between Cu(II) and His6, His13, and His14, to which an amide O (of Ala2) is also coordinated. His-His-His-His-His-His 56-60 amyloid beta precursor protein Homo sapiens 16-21 22191059-7 2011 The computed reduction potential that closely matches the experimental value for Cu(II)/Abeta corresponds to reduction of Component II (without Ala2) to the Cu(I) complex after endergonic attachment of His6. His-His-His-His-His-His 202-206 amyloid beta precursor protein Homo sapiens 81-93 21055759-4 2010 The progress of batch and adsorptive detagging by DAPase of maltose binding protein (MBP) tagged with two variants of hexa-histidine fusion tag was successfully monitored using cationic exchange chromatography. His-His-His-His-His-His 118-132 myelin basic protein Homo sapiens 85-88 20730478-2 2010 This study first optimized the conditions for efficient hexahistidine tagged human ADC (hisADC) gene delivery into mouse fibroblast cell line (NIH3T3) using retroviral vector (pLXSN). His-His-His-His-His-His 56-69 antizyme inhibitor 2 Homo sapiens 83-86 20668749-3 2010 All STAT6 analogs carry a modified C-terminus, which comprises the key phosphorylation site (Tyr641) responsible for homodimerization via reciprocal SH2 domain binding, a fluorescence label and a hexahistidine tag. His-His-His-His-His-His 196-209 signal transducer and activator of transcription 6 Homo sapiens 4-9 20703836-4 2010 Using conventional triple resonance experiments, (1)H (15)N and (13)C assignment of recombinant N terminal hexa-histidine tagged Darcin has been achieved. His-His-His-His-His-His 107-121 major urinary protein 20 Mus musculus 129-135 21063907-1 2010 Photosystem II from transplastomic plants of Nicotiana tabacum with a hexahistidine tag at the N-terminal end of the PsbE subunit (alpha-chain of the cytochrome b(559)) was purified according to the protocol of Fey et al. His-His-His-His-His-His 70-83 cytochrome b559 alpha subunit Nicotiana tabacum 117-121 20667476-7 2010 To this end, we developed procedures for higher level expression in insect cells of active recombinant CHK with a hexa-histidine tag attached to its C-terminus (referred to as CHK-His(6)) and its rapid purification by a two-step method. His-His-His-His-His-His 114-128 megakaryocyte-associated tyrosine kinase Homo sapiens 103-106 20667476-7 2010 To this end, we developed procedures for higher level expression in insect cells of active recombinant CHK with a hexa-histidine tag attached to its C-terminus (referred to as CHK-His(6)) and its rapid purification by a two-step method. His-His-His-His-His-His 114-128 megakaryocyte-associated tyrosine kinase Homo sapiens 176-179 20194360-2 2010 Here, we studied its role in the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by focusing on a psbK disruptant with hexahistidine-tagged CP47. His-His-His-His-His-His 136-149 psbK Thermosynechococcus elongatus BP-1 115-119 20044482-3 2010 Hexahistidine-tagged rat TRPV4 (His-rTRPV4) was solubilized with detergent and purified through affinity chromatography and size-exclusion chromatography. His-His-His-His-His-His 0-13 transient receptor potential cation channel, subfamily V, member 4 Rattus norvegicus 25-30 20044482-3 2010 Hexahistidine-tagged rat TRPV4 (His-rTRPV4) was solubilized with detergent and purified through affinity chromatography and size-exclusion chromatography. His-His-His-His-His-His 0-13 transient receptor potential cation channel, subfamily V, member 4 Rattus norvegicus 36-42 20131867-7 2010 Recombinant C-terminal hexa-histidine-tagged hCB1 (His6-hCB1) was expressed in cultured insect (Spodoptera frugiperda) cells, solubilized by a procedure devised to enhance receptor purity following metal-affinity chromatography, desalted by buffer exchange, and digested in solution with (chymo)trypsin. His-His-His-His-His-His 23-37 cannabinoid receptor 1 Homo sapiens 45-49 20131867-7 2010 Recombinant C-terminal hexa-histidine-tagged hCB1 (His6-hCB1) was expressed in cultured insect (Spodoptera frugiperda) cells, solubilized by a procedure devised to enhance receptor purity following metal-affinity chromatography, desalted by buffer exchange, and digested in solution with (chymo)trypsin. His-His-His-His-His-His 23-37 cannabinoid receptor 1 Homo sapiens 56-60 20811687-4 2010 In this study, human NKG2F recombinant expression in E. coli was carried out by using pET-28a with a hexahistidine (6x His) tag and a thrombin digestion sequence to the N-terminus of the recombinant protein NKG2F. His-His-His-His-His-His 101-114 killer cell lectin like receptor C4 Homo sapiens 21-26 20621464-1 2010 Label-free detection of the interaction between hexahistidine-tagged human kappa-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. His-His-His-His-His-His 48-61 opioid receptor kappa 1 Homo sapiens 75-96 20644585-2 2010 For the functional study of this protein, we obtained recombinant haponin with an N-terminal hexahistidine tag using a baculovirus expression system. His-His-His-His-His-His 93-106 eukaryotic translation initiation factor 1A domain containing Homo sapiens 66-73 20057140-6 2010 The C-terminal catalytic segment of the human Frk kinase conjugating hexahistidine purification tag (His-tag) was expressed in Escherichia coli. His-His-His-His-His-His 69-82 fyn related Src family tyrosine kinase Homo sapiens 46-49 19785040-1 2010 This study describes the use of a hexa-histidine tagged exopeptidase for the cleavage of hexa-histidine tags from recombinant maltose binding protein (MBP) when both tagged species are bound to an immobilized metal affinity chromatography (IMAC) matrix. His-His-His-His-His-His 34-48 myelin basic protein Homo sapiens 151-154 19785040-4 2010 In the presence of 50 mM imidazole, 46% of the originally adsorbed hexa-histidine tagged MBP was cleaved, released from the column, and recovered in a sample containing 100% native (i.e., completely detagged) MBP. His-His-His-His-His-His 67-81 myelin basic protein Homo sapiens 89-92 19785040-4 2010 In the presence of 50 mM imidazole, 46% of the originally adsorbed hexa-histidine tagged MBP was cleaved, released from the column, and recovered in a sample containing 100% native (i.e., completely detagged) MBP. His-His-His-His-His-His 67-81 myelin basic protein Homo sapiens 209-212 19795840-3 2009 Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)(6)] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. His-His-His-His-His-His 86-99 CEA cell adhesion molecule 3 Homo sapiens 0-24 19785040-6 2010 At higher imidazole concentrations, binding of both hexa-histidine tagged MBP and DAPase to the column was minimized, leading to characteristics of cleavage more closely resembling that of a batch cleavage. His-His-His-His-His-His 52-66 myelin basic protein Homo sapiens 74-77 19927318-8 2010 This is attributed to the controlled orientation and monolayer surface coverage of the His(6)-immobilized TK. His-His-His-His-His-His 87-93 transketolase Homo sapiens 106-108 20075618-5 2009 A highly purified and soluble CDX2 protein was obtained in E. coli strain BL21(DE3)RIL and a hexahistidine fusion system using Ni-NTA affinity column, anion exchange, and gel filtration chromatography. His-His-His-His-His-His 93-106 caudal type homeobox 2 Homo sapiens 30-34 19795840-3 2009 Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)(6)] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. His-His-His-His-His-His 86-99 CEA cell adhesion molecule 3 Homo sapiens 26-29 19795840-3 2009 Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)(6)] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. His-His-His-His-His-His 284-297 CEA cell adhesion molecule 3 Homo sapiens 0-24 19795840-3 2009 Carcinoembryonic antigen (CEA)-binding single chain variable fragments (scFvs) with a hexahistidine tag [(his)(6)] were synthesized using genetic engineering, and ordered immobilization of anti-CEA ScFvs on Ni nanoparticles was achieved by exploiting the specific interaction between hexahistidine and Ni. His-His-His-His-His-His 284-297 CEA cell adhesion molecule 3 Homo sapiens 26-29 19156821-5 2009 To lay a foundation for understanding of the molecular mechanism underlying functional diversity of the isoforms, we have carried out a comprehensive biochemical and biophysical analysis of purified hexahistidine-tagged EcRA and EcRB1 NTDs (EcRA-NTD and EcRB1-NTD). His-His-His-His-His-His 199-212 Ecdysone receptor Drosophila melanogaster 220-224 19664997-1 2009 We have previously expressed hexa-histidine-tagged human glutathione transferase GST T1-1 at very high levels in an Escherichia colilacZ mutagenicity assay strain. His-His-His-His-His-His 29-43 CD2 molecule Homo sapiens 85-89 19650657-1 2009 Nitrilotriacetic acid (NTA) has moderate affinity (10 muM) for hexahistidine (His6) and is widely used in the purification of His6-tagged proteins. His-His-His-His-His-His 63-76 latexin Homo sapiens 54-57 19650657-1 2009 Nitrilotriacetic acid (NTA) has moderate affinity (10 muM) for hexahistidine (His6) and is widely used in the purification of His6-tagged proteins. His-His-His-His-His-His 78-82 latexin Homo sapiens 54-57 19156821-5 2009 To lay a foundation for understanding of the molecular mechanism underlying functional diversity of the isoforms, we have carried out a comprehensive biochemical and biophysical analysis of purified hexahistidine-tagged EcRA and EcRB1 NTDs (EcRA-NTD and EcRB1-NTD). His-His-His-His-His-His 199-212 Ecdysone receptor Drosophila melanogaster 241-245 19330375-3 2009 Tagged antibody fragments scFv-Cys7-His6, His6-scFv-Cys7, and Fab-Cys7 lost considerable affinity for the antigen; however, Fab-His6, Fab-Cys1, and scFv-His6-Cys1 were able to detect immobilized antigen, revealing that the position and number of histidine and cysteine residues are involved differently in the reactivity of antibody fragments. His-His-His-His-His-His 42-46 immunglobulin heavy chain variable region Homo sapiens 47-51 19330375-3 2009 Tagged antibody fragments scFv-Cys7-His6, His6-scFv-Cys7, and Fab-Cys7 lost considerable affinity for the antigen; however, Fab-His6, Fab-Cys1, and scFv-His6-Cys1 were able to detect immobilized antigen, revealing that the position and number of histidine and cysteine residues are involved differently in the reactivity of antibody fragments. His-His-His-His-His-His 42-46 immunglobulin heavy chain variable region Homo sapiens 47-51 19330375-3 2009 Tagged antibody fragments scFv-Cys7-His6, His6-scFv-Cys7, and Fab-Cys7 lost considerable affinity for the antigen; however, Fab-His6, Fab-Cys1, and scFv-His6-Cys1 were able to detect immobilized antigen, revealing that the position and number of histidine and cysteine residues are involved differently in the reactivity of antibody fragments. His-His-His-His-His-His 36-40 immunglobulin heavy chain variable region Homo sapiens 26-30 19330375-3 2009 Tagged antibody fragments scFv-Cys7-His6, His6-scFv-Cys7, and Fab-Cys7 lost considerable affinity for the antigen; however, Fab-His6, Fab-Cys1, and scFv-His6-Cys1 were able to detect immobilized antigen, revealing that the position and number of histidine and cysteine residues are involved differently in the reactivity of antibody fragments. His-His-His-His-His-His 42-46 immunglobulin heavy chain variable region Homo sapiens 47-51 19330375-3 2009 Tagged antibody fragments scFv-Cys7-His6, His6-scFv-Cys7, and Fab-Cys7 lost considerable affinity for the antigen; however, Fab-His6, Fab-Cys1, and scFv-His6-Cys1 were able to detect immobilized antigen, revealing that the position and number of histidine and cysteine residues are involved differently in the reactivity of antibody fragments. His-His-His-His-His-His 42-46 immunglobulin heavy chain variable region Homo sapiens 47-51 19330375-3 2009 Tagged antibody fragments scFv-Cys7-His6, His6-scFv-Cys7, and Fab-Cys7 lost considerable affinity for the antigen; however, Fab-His6, Fab-Cys1, and scFv-His6-Cys1 were able to detect immobilized antigen, revealing that the position and number of histidine and cysteine residues are involved differently in the reactivity of antibody fragments. His-His-His-His-His-His 42-46 immunglobulin heavy chain variable region Homo sapiens 47-51 19330375-3 2009 Tagged antibody fragments scFv-Cys7-His6, His6-scFv-Cys7, and Fab-Cys7 lost considerable affinity for the antigen; however, Fab-His6, Fab-Cys1, and scFv-His6-Cys1 were able to detect immobilized antigen, revealing that the position and number of histidine and cysteine residues are involved differently in the reactivity of antibody fragments. His-His-His-His-His-His 42-46 immunglobulin heavy chain variable region Homo sapiens 47-51 19330375-5 2009 The best orientation performance was obtained with Fab-Cys1-biotin on streptavidin-coated plates with increased signal levels of 62%, while oriented immobilization of Fab-His6 and scFv-His6-Cys1 on nickel- and maleimide-coated plates failed to improve the ELISA sensitivity. His-His-His-His-His-His 171-175 FA complementation group B Homo sapiens 167-170 19330375-5 2009 The best orientation performance was obtained with Fab-Cys1-biotin on streptavidin-coated plates with increased signal levels of 62%, while oriented immobilization of Fab-His6 and scFv-His6-Cys1 on nickel- and maleimide-coated plates failed to improve the ELISA sensitivity. His-His-His-His-His-His 185-189 immunglobulin heavy chain variable region Homo sapiens 180-184 19330375-5 2009 The best orientation performance was obtained with Fab-Cys1-biotin on streptavidin-coated plates with increased signal levels of 62%, while oriented immobilization of Fab-His6 and scFv-His6-Cys1 on nickel- and maleimide-coated plates failed to improve the ELISA sensitivity. His-His-His-His-His-His 185-189 cystin 1 Homo sapiens 190-194 19091820-5 2009 BSPH1 accumulates in inclusion bodies when expressed with an N-terminal hexahistidine tag in BL21 (DE3) Escherichia coli cells. His-His-His-His-His-His 72-85 binder of sperm protein homolog 1 Homo sapiens 0-5 19116169-5 2009 Here, we report a method of expressing soluble, hexahistidine-tagged full-length human Rab8A from E. coli. His-His-His-His-His-His 48-61 RAB8A, member RAS oncogene family Homo sapiens 87-92 19322475-7 2009 In the second model, both the N-terminus and the carboxylate functions of Asp1 are ligated together with the Ntau of His6 and of His13 (or His14). His-His-His-His-His-His 117-121 beta-secretase 2 Homo sapiens 74-78 18452279-2 2008 To characterize this key endocannabinoid enzyme by mass spectrometry-based proteomics, we first overexpressed recombinant hexa-histidine-tagged human MGL (hMGL) in Escherichia coli and purified it in a single chromatographic step with high yield (approximately 30 mg/L). His-His-His-His-His-His 122-136 monoglyceride lipase Homo sapiens 150-153 19478528-4 2009 METHODS: The resultant prokaryotic expression vector, pET-32a(+)/UL35, includes an amino-terminal His6 as a fusion partner. His-His-His-His-His-His 98-102 UL3.5 Anatid alphaherpesvirus 1 65-69 18721756-1 2008 The active site of recombinant hexa-histidine-tagged human monoacylglycerol lipase (hMGL) is characterized by mass spectrometry using the inhibitors 5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide (AM6701), and N-arachidonylmaleimide (NAM) as probes. His-His-His-His-His-His 31-45 monoglyceride lipase Homo sapiens 84-88 18467498-6 2008 This vector produces a fusion protein (denoted as His(6)-Smt3-X) in which the protein of interest (X) is fused to a hexahistidine (His(6))-tagged Smt3. His-His-His-His-His-His 116-129 SUMO family protein SMT3 Saccharomyces cerevisiae S288C 57-61 18467498-6 2008 This vector produces a fusion protein (denoted as His(6)-Smt3-X) in which the protein of interest (X) is fused to a hexahistidine (His(6))-tagged Smt3. His-His-His-His-His-His 116-129 SUMO family protein SMT3 Saccharomyces cerevisiae S288C 146-150 18992717-5 2009 We expressed the rCYP26A1 in baculovirus-infected insect cells and purified the hexahistidine tagged protein to homogeneity. His-His-His-His-His-His 80-93 cytochrome P450, family 26, subfamily a, polypeptide 1 Rattus norvegicus 17-25 19129647-1 2009 Human macrophage dectin-1, a type II transmembrane beta-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and assayed for binding specificity. His-His-His-His-His-His 126-139 C-type lectin domain containing 7A Homo sapiens 17-25 20011663-4 2009 The recombinant protein, IL-33 containing a hexahistidine tag in the C-terminal, was expressed in Escherichia coli. His-His-His-His-His-His 44-57 interleukin 33 Mus musculus 25-30 18949319-2 2008 In this study, a hexahistidine-tagged recombinant protein corresponding to residues 5-190 of the N-terminal of Plasmodium falciparum RAP2 (rN-RAP2) was produced using a new method of solubilization and purification. His-His-His-His-His-His 17-30 RAP2A, member of RAS oncogene family Homo sapiens 133-137 18949319-2 2008 In this study, a hexahistidine-tagged recombinant protein corresponding to residues 5-190 of the N-terminal of Plasmodium falciparum RAP2 (rN-RAP2) was produced using a new method of solubilization and purification. His-His-His-His-His-His 17-30 RAP2A, member of RAS oncogene family Homo sapiens 142-146 18721756-1 2008 The active site of recombinant hexa-histidine-tagged human monoacylglycerol lipase (hMGL) is characterized by mass spectrometry using the inhibitors 5-((biphenyl-4-yl)methyl)-N,N-dimethyl-2H-tetrazole-2-carboxamide (AM6701), and N-arachidonylmaleimide (NAM) as probes. His-His-His-His-His-His 31-45 monoglyceride lipase Homo sapiens 59-82 18452279-2 2008 To characterize this key endocannabinoid enzyme by mass spectrometry-based proteomics, we first overexpressed recombinant hexa-histidine-tagged human MGL (hMGL) in Escherichia coli and purified it in a single chromatographic step with high yield (approximately 30 mg/L). His-His-His-His-His-His 122-136 monoglyceride lipase Homo sapiens 155-159 17571853-7 2008 In the present work, we successfully expressed and purified soluble recombinant H1C in an Escherichia coli expression system using a hexahistidine tag fusion strategy and providing tRNAs for rare codons. His-His-His-His-His-His 133-146 H1.2 linker histone, cluster member Homo sapiens 80-83 18466682-7 2008 RESULTS: About 18.33 mg His6-Eppin was obtained from 1-L flask culture. His-His-His-His-His-His 24-28 epididymal peptidase inhibitor Homo sapiens 29-34 20641278-0 2004 (111)In-Tetraazacyclododecane-N,N",N"",N"""-tetraacetic acid-HHHHHH-EAYGWMDF-NH2 peptide (111)In-Tetraazacyclododecane-N,N",N"",N"""-tetraacetic acid-HHHHHH-EAYGWMDF-NH2 peptide ((111)In-DOTA-H6-Met) is a radiolabeled gastrin analog that can be used for single-photon emission computed tomography (SPECT) imaging of tumors that express the gastrin/cholecystokinin-2 (CCK-2) receptor (1). His-His-His-His-His-His 61-67 gastrin Rattus norvegicus 218-225 20641278-0 2004 (111)In-Tetraazacyclododecane-N,N",N"",N"""-tetraacetic acid-HHHHHH-EAYGWMDF-NH2 peptide (111)In-Tetraazacyclododecane-N,N",N"",N"""-tetraacetic acid-HHHHHH-EAYGWMDF-NH2 peptide ((111)In-DOTA-H6-Met) is a radiolabeled gastrin analog that can be used for single-photon emission computed tomography (SPECT) imaging of tumors that express the gastrin/cholecystokinin-2 (CCK-2) receptor (1). His-His-His-His-His-His 61-67 gastrin Rattus norvegicus 340-347 20641278-0 2004 (111)In-Tetraazacyclododecane-N,N",N"",N"""-tetraacetic acid-HHHHHH-EAYGWMDF-NH2 peptide (111)In-Tetraazacyclododecane-N,N",N"",N"""-tetraacetic acid-HHHHHH-EAYGWMDF-NH2 peptide ((111)In-DOTA-H6-Met) is a radiolabeled gastrin analog that can be used for single-photon emission computed tomography (SPECT) imaging of tumors that express the gastrin/cholecystokinin-2 (CCK-2) receptor (1). His-His-His-His-His-His 61-67 cholecystokinin B receptor Rattus norvegicus 367-382 18227063-7 2008 To study BcrR binding to this region, we over-produced BcrR with a C-terminal hexa-histidine tag in Escherichia coli membranes, extracted the protein with n-dodecyl-beta-d-maltoside, and subsequently purified it via Ni(2+)-nitrilotriacetic acid and gel filtration chromatography to apparent homogeneity. His-His-His-His-His-His 78-92 DNA-binding protein Enterococcus faecalis 55-59 17997327-2 2008 We have used a pET-ubiquitin expression system to produce respiratory syncytial virus (RSV) NS1 protein in E. coli that contains a hexahistidine-tag on either the amino- or carboxyl-terminus (His(6)-NS1 and NS1-His(6), respectively). His-His-His-His-His-His 131-144 non-structural protein 1 (1C) Respiratory syncytial virus 92-95 17997327-4 2008 Generation of recombinant RSV indicated that addition of the hexahistidine tag to the C-terminus of NS1 slightly decreased viral replication competence whereas addition of the tag to the N-terminus had no observable effect. His-His-His-His-His-His 61-74 non-structural protein 1 (1C) Respiratory syncytial virus 100-103 18272395-4 2008 For the purpose of crystallization, C-terminal catalytic segment of human Lyn kinase conjugating hexahistidine purification tag (His-tag) was expressed in Sf21 insect cells. His-His-His-His-His-His 97-110 LYN proto-oncogene, Src family tyrosine kinase Homo sapiens 74-77 18284706-5 2008 Wild type full-length p53 protein was produced in fusion with biotin carboxyl carrier peptide (BCCP) or hexahistidine [(His)6] using pAK400 and pET15b(+) vectors, respectively. His-His-His-His-His-His 104-117 tumor protein p53 Homo sapiens 22-25 17576199-5 2008 A rat brain Ngb gene was cloned and fused with a gene fragment encoding the nine-amino-acid TAT PTD (transactivator-of-transcription protein-transduction domain; RKKRRQRRR) of HIV-1 in a prokaryotic expression vector to generate a genetic in-frame N-terminal hexahistidine-tagged) TAT PTD-Ngb fusion protein. His-His-His-His-His-His 259-272 neuroglobin Rattus norvegicus 12-15 17587159-0 2007 Expression, purification and functional analysis of hexahistidine-tagged human glutathione S-transferase P1-1 and its cysteinyl mutants. His-His-His-His-His-His 52-65 S100 calcium binding protein A10 Homo sapiens 105-109 17561413-5 2007 A B(2) receptor tagged with a hexahistidine at the N-terminus and a nonapeptide at the C-terminus was selected for high expression level and preserved ligand-binding characteristics. His-His-His-His-His-His 30-43 bradykinin receptor B2 Homo sapiens 2-15 17583737-5 2007 The cDNA sequence of LL-DAP-AT from Arabidopsis thaliana (AtDAP-AT) was optimized for expression in bacteria and cloned in Escherichia coli without its leader sequence but with a C-terminal hexahistidine affinity tag to aid protein purification. His-His-His-His-His-His 190-203 LL-diaminopimelate aminotransferase Arabidopsis thaliana 21-30 17587159-1 2007 The bacterial expression and purification of human glutathione S-transferase P1-1(hGST P1-1), as a hexahistidine-tagged polypeptide was performed. His-His-His-His-His-His 99-112 S100 calcium binding protein A10 Homo sapiens 77-81 17587159-1 2007 The bacterial expression and purification of human glutathione S-transferase P1-1(hGST P1-1), as a hexahistidine-tagged polypeptide was performed. His-His-His-His-His-His 99-112 glutathione S-transferase pi 1 Homo sapiens 82-91 17587159-4 2007 Moreover, on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions, hexahistidine-tagged hGST P1-1 (His(6)-hGST P1-1) treated with 1 mM H(2)O(2) showed at least three extra bands, in addition to the native His(6)-hGST P1-1 subunit band. His-His-His-His-His-His 93-106 glutathione S-transferase pi 1 Homo sapiens 114-123 17587159-4 2007 Moreover, on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions, hexahistidine-tagged hGST P1-1 (His(6)-hGST P1-1) treated with 1 mM H(2)O(2) showed at least three extra bands, in addition to the native His(6)-hGST P1-1 subunit band. His-His-His-His-His-His 93-106 S100 calcium binding protein A10 Homo sapiens 119-123 17587159-4 2007 Moreover, on SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions, hexahistidine-tagged hGST P1-1 (His(6)-hGST P1-1) treated with 1 mM H(2)O(2) showed at least three extra bands, in addition to the native His(6)-hGST P1-1 subunit band. His-His-His-His-His-His 93-106 glutathione S-transferase pi 1 Homo sapiens 132-141 17623110-4 2007 RESULTS: To characterize the biochemical properties of JEV RdRp, we expressed in Escherichia coli and purified an enzymatically active full-length recombinant JEV NS5 protein with a hexahistidine tag at the N-terminus. His-His-His-His-His-His 182-195 Raf-1 proto-oncogene, serine/threonine kinase Homo sapiens 163-166 17194602-2 2007 In this study, the recombinant human IFN-lambda1 containing a hexahistidine tag was expressed in Escherichia coli. His-His-His-His-His-His 62-75 interferon lambda 1 Homo sapiens 37-48 17181532-2 2007 In the present paper, the cloning of the ctgf gene from human umbilical-vein endothelial cells and expression of the protein in Escherichia coli as an N-terminal hexahistidine fusion protein is described. His-His-His-His-His-His 162-175 cellular communication network factor 2 Homo sapiens 41-45 17154546-1 2006 Treatment of an N-terminal-containing His6-tagged insulysin (His6-IDE) with proteinase K led to the initial cleavage of the His tag and linker region. His-His-His-His-His-His 38-42 insulin degrading enzyme Homo sapiens 50-59 17244490-3 2007 The expressed fusion protein, H(6)NtHSP70-2 (hexahistidine-tagged Nicotiana tabacum heat shock protein 70-2), maintained the stability of E. coli proteins up to 90 degrees C. Measuring the light scattering of luciferase (luc) revealed that NtHSP70-2 prevents the aggregation of luc without ATP during high-temperature stress. His-His-His-His-His-His 45-58 heat shock 70 kDa protein Nicotiana tabacum 84-105 17196395-4 2007 Overexpression of a hexahistidine-tagged cysteine-rich LOX-1 extracellular domain in bacteria leads to the formation of aggregates that accumulated in bacterial inclusion bodies. His-His-His-His-His-His 20-33 oxidized low density lipoprotein receptor 1 Homo sapiens 55-60 17196395-5 2007 The hexahistidine-tagged LOX-1 molecule was purified by affinity chromatography from solubilized inclusion bodies. His-His-His-His-His-His 4-17 oxidized low density lipoprotein receptor 1 Homo sapiens 25-30 17154546-1 2006 Treatment of an N-terminal-containing His6-tagged insulysin (His6-IDE) with proteinase K led to the initial cleavage of the His tag and linker region. His-His-His-His-His-His 38-42 insulin degrading enzyme Homo sapiens 66-69 16750393-4 2006 In an attempt to make SycD amenable to structural investigations we recombinantly expressed SycD with a hexahistidine tag in Escherichia coli. His-His-His-His-His-His 104-117 sycD Yersinia enterocolitica 92-96 16904956-0 2006 The effect of the hexahistidine-tag in the oligomerization of HSC70 constructs. His-His-His-His-His-His 18-31 heat shock protein family A (Hsp70) member 8 Homo sapiens 62-67 17192193-3 2006 In order to gain insight into the structure/function properties of DGAT1 from Brassica napus (BnDGAT1), we produced and partially characterized a recombinant polyHis-tagged N-terminal fragment of the enzyme, BnDGAT1(1-116)His6, with calculated molecular mass of 13,278 Da. His-His-His-His-His-His 222-226 diacylglycerol O-acyltransferase 1-like Brassica napus 67-72 17192193-3 2006 In order to gain insight into the structure/function properties of DGAT1 from Brassica napus (BnDGAT1), we produced and partially characterized a recombinant polyHis-tagged N-terminal fragment of the enzyme, BnDGAT1(1-116)His6, with calculated molecular mass of 13,278 Da. His-His-His-His-His-His 222-226 diacylglycerol O-acyltransferase 1-like Brassica napus 94-101 17192193-4 2006 RESULTS: BnDGAT1(1-116)His6 was highly purified from bacterial lysate and plate-like monoclinic crystals were grown using this preparation. His-His-His-His-His-His 23-27 diacylglycerol O-acyltransferase 1-like Brassica napus 9-16 17192193-5 2006 Lipidex-1000 binding assays and gel electrophoresis indicated that BnDGAT1(1-116)His6 interacts with long chain acyl-CoA. His-His-His-His-His-His 81-85 diacylglycerol O-acyltransferase 1-like Brassica napus 67-74 17192193-7 2006 Gel filtration chromatography and cross-linking studies indicated that BnDGAT1(1-116)His6 self-associated to form a tetramer. His-His-His-His-His-His 85-89 diacylglycerol O-acyltransferase 1-like Brassica napus 71-78 17192193-8 2006 Polyclonal antibodies raised against a peptide of 15 amino acid residues representing a segment of BnDGAT1(1-116)His6 failed to react with protein in microsomal vesicles following treatment with proteinase K, suggesting that the N-terminal fragment of BnDGAT1 was localized to the cytosolic side of the ER. His-His-His-His-His-His 113-117 diacylglycerol O-acyltransferase 1-like Brassica napus 99-106 17098188-4 2006 An N-terminal hexahistidine-tagged ABCG2(R482G) isoform was expressed to high levels in insect cells. His-His-His-His-His-His 14-27 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 35-40 16615111-10 2006 Besides His6, His13, and His14, Tyr10 is also involved in the coordination of Abeta(1-16) with Cu2+, which is supported by 1H NMR and UV-visible absorption spectra. His-His-His-His-His-His 8-12 amyloid beta precursor protein Homo sapiens 78-83 16527369-2 2006 The nsp4 proteinase was expressed either fused to maltose binding protein or carrying a C-terminal hexahistidine tag. His-His-His-His-His-His 99-112 serine protease 57 Homo sapiens 4-8 16527369-2 2006 The nsp4 proteinase was expressed either fused to maltose binding protein or carrying a C-terminal hexahistidine tag. His-His-His-His-His-His 99-112 endogenous retrovirus group K member 8, envelope Homo sapiens 9-19 16343940-4 2006 Forty-two milligrams alpha-glucosidase was purified from 3.5 L culture in four steps applying an N-terminal hexa-histidine tag. His-His-His-His-His-His 108-122 Agl1 Hordeum vulgare 21-38 16651871-5 2006 Mature alpha(2)AP was expressed as a hexahistidine-tagged recombinant protein in Escherichia coli, purified by nickel-chelate affinity and ion exchange chromatography, and its reaction with plasmin and soluble fibrin assessed electrophoretically and compared with an analogous recombinant human alpha(2)AP. His-His-His-His-His-His 37-50 alpha-2-antiplasmin Oryctolagus cuniculus 7-17 16510293-4 2006 Recombinant K1-5 efficiently expressed in E. coli BL21 after IPTG induction was monitored by SDS-PAGE and Western blotting with an anti-angiostatin monoclonal antibody and an anti-hexahistidine tag antibody. His-His-His-His-His-His 180-193 RBPJ pseudogene 3 Homo sapiens 12-16 16598021-5 2006 A C-terminal, hexahistidine tag fusion enabled the identification of PslD. His-His-His-His-His-His 14-27 biofilm formation protein PslD Pseudomonas aeruginosa PAO1 69-73 16847575-1 2006 Human ABCG2 was efficiently overexpressed in insect cell membranes, solubilized with 3-[(3-cholamidopropyl)dimethyl ammonio]-1-propanesulfonate, and purified through N-terminal hexahistidine tag. His-His-His-His-His-His 177-190 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 6-11 16439156-3 2006 Recombinant preS1 protein with an N-terminal His6 tag was expressed at high levels in soluble form, yielding about 44% of the total cellular protein. His-His-His-His-His-His 45-49 large envelope protein;middle envelope protein;small envelope protein Hepatitis B virus 12-17 16755930-4 2006 A prokaryotic expression vector for soluble human CD40 ectodomain fused with an IZ motif and a hexa-histidine (His6) tag at its carboxyl terminus (sCD40IZ) was constructed by multiple round PCR using cloned CD40 cDNA as a template. His-His-His-His-His-His 95-109 CD40 molecule Homo sapiens 50-54 16242957-3 2006 This report presents a procedure to obtain and purify recombinant PrP from Xenopus laevis expressed in Escherichia coli as a fusion protein in which mature PrP (residues 21-194) is linked to a 35-amino acid N-terminal extension containing a hexahistidine stretch. His-His-His-His-His-His 241-254 prion protein S homeolog Xenopus laevis 66-69 16242957-3 2006 This report presents a procedure to obtain and purify recombinant PrP from Xenopus laevis expressed in Escherichia coli as a fusion protein in which mature PrP (residues 21-194) is linked to a 35-amino acid N-terminal extension containing a hexahistidine stretch. His-His-His-His-His-His 241-254 prion protein S homeolog Xenopus laevis 156-159 16533060-5 2006 We now report full restoration of biological activity to the isolated tissue factor ectodomain via the engineering of a hexahistidine tag onto its C-terminus and its use in combination with membrane bilayers containing nickel-chelating lipids. His-His-His-His-His-His 120-133 coagulation factor III, tissue factor Homo sapiens 70-83 16513621-15 2006 CONCLUSION: The use of Tc(I)-carbonyl chemistry enabled us to prepare a stable, site-specifically labeled 99mTc-His6-(ZHER2:4)2 conjugate that was able to bind to HER2-expressing cells in vitro and in vivo. His-His-His-His-His-His 112-116 erb-b2 receptor tyrosine kinase 2 Homo sapiens 119-123 16421447-2 2006 The crystal structure of a construct of E. coli DraD possessing an additional C-terminal extension of 13 amino acids, including a His6 tag, has been solved at a resolution of 1.05 angstroms. His-His-His-His-His-His 130-134 EGF containing fibulin extracellular matrix protein 1 Homo sapiens 48-52 16287262-3 2005 A series of seven hexahistidine tags with tyrosines placed in various positions (H(6)Y tags) were added to the amino terminus of the I28 immunoglobulin domain of the human cardiac titin. His-His-His-His-His-His 18-31 titin Homo sapiens 180-185 16401067-2 2006 We now demonstrate that a heterodimer complex is formed between 1-Cys Prx with a C-terminal His6 tag and GST pi upon incubation of the two proteins at pH 8.0 in buffer containing 20% 1,6-hexanediol to dissociate the homodimers, followed by dialysis against buffer containing 2.5 mM glutathione (GSH) but lacking 1,6-hexanediol. His-His-His-His-His-His 92-96 peroxiredoxin 6 Homo sapiens 64-73 16080185-4 2005 Digestion of the hexahistidine tag of MBP-His(6) by Factor Xa and HT15-MBP by DAPase-1 was successful. His-His-His-His-His-His 17-30 myelin basic protein Homo sapiens 38-41 16080185-4 2005 Digestion of the hexahistidine tag of MBP-His(6) by Factor Xa and HT15-MBP by DAPase-1 was successful. His-His-His-His-His-His 17-30 coagulation factor X Homo sapiens 52-61 16489921-1 2006 The catalytic properties of organophosphate hydrolase (OPH) containing a hexahistidine tag His6 (His6-OPH) and purified to 98% homogeneity were investigated. His-His-His-His-His-His 73-86 acylaminoacyl-peptide hydrolase Homo sapiens 28-53 16489921-1 2006 The catalytic properties of organophosphate hydrolase (OPH) containing a hexahistidine tag His6 (His6-OPH) and purified to 98% homogeneity were investigated. His-His-His-His-His-His 73-86 acylaminoacyl-peptide hydrolase Homo sapiens 55-58 16489921-1 2006 The catalytic properties of organophosphate hydrolase (OPH) containing a hexahistidine tag His6 (His6-OPH) and purified to 98% homogeneity were investigated. His-His-His-His-His-His 91-95 acylaminoacyl-peptide hydrolase Homo sapiens 28-53 16489921-1 2006 The catalytic properties of organophosphate hydrolase (OPH) containing a hexahistidine tag His6 (His6-OPH) and purified to 98% homogeneity were investigated. His-His-His-His-His-His 91-95 acylaminoacyl-peptide hydrolase Homo sapiens 55-58 16202624-5 2006 Expression of mLACTB as a C-terminal GST fusion protein or with either an N- or C-terminal His6-tag resulted in proteolytic degradation of the protein and we were not able to detect full-length mLACTB. His-His-His-His-His-His 91-95 lactamase, beta Mus musculus 14-20 16169847-6 2005 A stable cell line has been produced that incorporates hexahistidine-labeled p43 into the multisynthetase complex. His-His-His-His-His-His 55-68 aminoacyl tRNA synthetase complex interacting multifunctional protein 1 Homo sapiens 77-80 16169218-2 2005 This study, together with our earlier report on 5-BrAtc, demonstrated the importance of replacing His6 with phenyl-containing rigid templates in achieving good hMC4R agonist potency and selectivity against hMC1R in linear pentapeptides. His-His-His-His-His-His 98-102 melanocortin 4 receptor Homo sapiens 160-165 15699485-4 2005 Polyubiquitin conjugates from yeast cells that express hexahistidine-tagged ubiquitin (H6-ubiquitin) were first enriched on a polyubiquitin binding protein affinity resin. His-His-His-His-His-His 55-68 ubiquitin Saccharomyces cerevisiae S288C 4-13 19003060-4 2005 CHO-S cells were selected to stably express full-length recombinant human uPA containing a hexahistidine tag at its C-terminus to permit purification by nickel-based affinity chromatography. His-His-His-His-His-His 91-104 plasminogen activator, urokinase Homo sapiens 74-77 15899373-3 2005 The DUE-B protein was expressed with an added C-terminal sequence of six adjacent histidine residues, a His6-tag and immobilized on a chiral ligand exchange support, the CLC-L column, using Ni2+ as the coordinating metal ion. His-His-His-His-His-His 104-108 D-aminoacyl-tRNA deacylase 1 Homo sapiens 4-9 15980016-6 2005 Kinetics measurements made by surface plasmon resonance, using purified Fab (antigen-binding fragment) to prevent avidity effects, demonstrate that the selected peptides bind with 10- to 75-fold higher affinities than a hexahistidine peptide. His-His-His-His-His-His 220-233 FA complementation group B Homo sapiens 72-75 15699485-4 2005 Polyubiquitin conjugates from yeast cells that express hexahistidine-tagged ubiquitin (H6-ubiquitin) were first enriched on a polyubiquitin binding protein affinity resin. His-His-His-His-His-His 55-68 ubiquitin Saccharomyces cerevisiae S288C 76-85 15555936-3 2004 One prototype candidate consisting of MBP::VP6::His6, a chimeric protein of maltose-binding protein, VP6 and hexahistidine, was expressed mainly as truncated polypeptides in Escherichia coli BL21(DE3) cells. His-His-His-His-His-His 109-122 myelin basic protein Mus musculus 38-41 16511079-2 2005 Recombinant bovine uteroglobin (recbUG), consisting of 80 amino acids with a C-terminal His6 tag, was overexpressed in Escherichia coli and purified. His-His-His-His-His-His 88-92 secretoglobin family 1A member 1 Bos taurus 19-30 15575700-8 2004 Finally, successful folding of the cell-free synthesized GM-CSF-his6 was confirmed by its cell-proliferation activity after purification with a Ni2+ chelating column. His-His-His-His-His-His 64-68 colony stimulating factor 2 (granulocyte-macrophage) Mus musculus 57-63 15265046-8 2004 Complex I activity can be restored with ESSS subunits tagged with either hemagglutinin (HA) or hexahistidine (His6) epitopes at the C-terminus. His-His-His-His-His-His 95-108 NADH:ubiquinone oxidoreductase subunit B11 Homo sapiens 40-44 15265046-8 2004 Complex I activity can be restored with ESSS subunits tagged with either hemagglutinin (HA) or hexahistidine (His6) epitopes at the C-terminus. His-His-His-His-His-His 110-114 NADH:ubiquinone oxidoreductase subunit B11 Homo sapiens 40-44 15205352-4 2004 A novel metal-chelating lipid, 3(nitrilotriacetic acid)-ditetradecylamine, was incorporated into B16-OVA-derived PMV, allowing recombinant hexahistidine-tagged forms of single chain antibody fragments to the DC surface molecules CD11c and DEC-205, to be conveniently "engrafted" onto the vesicle surface by metal-chelating linkage. His-His-His-His-His-His 139-152 integrin alpha X Mus musculus 229-234 15205352-4 2004 A novel metal-chelating lipid, 3(nitrilotriacetic acid)-ditetradecylamine, was incorporated into B16-OVA-derived PMV, allowing recombinant hexahistidine-tagged forms of single chain antibody fragments to the DC surface molecules CD11c and DEC-205, to be conveniently "engrafted" onto the vesicle surface by metal-chelating linkage. His-His-His-His-His-His 139-152 lymphocyte antigen 75 Mus musculus 239-246 12637499-6 2003 Recombinant hexahistidine-tagged TagF protein was purified from Escherichia coli and was used to develop a novel membrane pelleting assay to monitor poly(glycerol phosphate) polymerase activity. His-His-His-His-His-His 12-25 CDP-glycerol:polyglycerol phosphate glycero-phosphotransferase (poly(glycerol phosphate) polymerase) Bacillus subtilis subsp. subtilis str. 168 33-37 15135403-6 2004 The cDNA coding for Mus musculus MTHFS was isolated and expressed in E. coli with a hexa-histidine tag. His-His-His-His-His-His 84-98 5, 10-methenyltetrahydrofolate synthetase Mus musculus 33-38 14766311-6 2004 Both plasmids led to the successful secretory expression of active, glycosylated GM2AP, which could easily be purified by Ni-NTA chromatography due to the hexahistidine tag introduced at the C-terminus. His-His-His-His-His-His 155-168 ganglioside GM2 activator Homo sapiens 81-86 14965310-1 2004 Angiotensin II (AII), Asp1-Arg2-Val3-Tyr4-Ile5-His6-Pro7-Phe8, the primary active hormone of the Renin-Angiotensin-System (RAS), is a major vasoconstrictor implicated in the cause of hypertension. His-His-His-His-His-His 47-51 arginase 2 Homo sapiens 27-31 14550646-0 2003 Purification and characterization of hexahistidine-tagged elongation factor SelB. His-His-His-His-His-His 37-50 eukaryotic elongation factor, selenocysteine-tRNA specific Homo sapiens 76-80 14550646-4 2003 In this report, a fast and efficient method for the purification of large amounts of hexahistidine-tagged SelB is presented. His-His-His-His-His-His 85-98 eukaryotic elongation factor, selenocysteine-tRNA specific Homo sapiens 106-110 12963338-3 2003 Both signal and structural fragment of interleukin-3 were cloned from this cDNA library, modified by adding a hexahistidine-tag at the C-terminus, and introduced into the pBacPAK9 transfer vector to generate recombinant baculoviruses. His-His-His-His-His-His 110-123 interleukin 3 Homo sapiens 39-52 12788385-4 2003 When cardiomyocytes expressing hexahistidine-tagged c-Src via adenoviral gene delivery were used for RGD stimulation, the expressed c-Src exhibited relocation: (i) biochemical analysis revealed c-Src movement from the detergent-soluble to the -insoluble cytoskeletal fraction and (ii) confocal microscopic analysis showed c-Src movement from a nuclear/perinuclear to a sarcolemmal region. His-His-His-His-His-His 31-44 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 52-57 12788385-4 2003 When cardiomyocytes expressing hexahistidine-tagged c-Src via adenoviral gene delivery were used for RGD stimulation, the expressed c-Src exhibited relocation: (i) biochemical analysis revealed c-Src movement from the detergent-soluble to the -insoluble cytoskeletal fraction and (ii) confocal microscopic analysis showed c-Src movement from a nuclear/perinuclear to a sarcolemmal region. His-His-His-His-His-His 31-44 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 132-137 12788385-4 2003 When cardiomyocytes expressing hexahistidine-tagged c-Src via adenoviral gene delivery were used for RGD stimulation, the expressed c-Src exhibited relocation: (i) biochemical analysis revealed c-Src movement from the detergent-soluble to the -insoluble cytoskeletal fraction and (ii) confocal microscopic analysis showed c-Src movement from a nuclear/perinuclear to a sarcolemmal region. His-His-His-His-His-His 31-44 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 132-137 12788385-4 2003 When cardiomyocytes expressing hexahistidine-tagged c-Src via adenoviral gene delivery were used for RGD stimulation, the expressed c-Src exhibited relocation: (i) biochemical analysis revealed c-Src movement from the detergent-soluble to the -insoluble cytoskeletal fraction and (ii) confocal microscopic analysis showed c-Src movement from a nuclear/perinuclear to a sarcolemmal region. His-His-His-His-His-His 31-44 SRC proto-oncogene, non-receptor tyrosine kinase Homo sapiens 132-137 15039571-5 2004 The DH/PH tandem of PDZ-RhoGEF and C-terminally truncated RhoA were overexpressed in Escherichia coli as TEV protease-cleavable fusion proteins containing GST and a hexahistidine tag at the N-termini, respectively. His-His-His-His-His-His 165-178 Rho guanine nucleotide exchange factor 11 Homo sapiens 24-30 15039571-5 2004 The DH/PH tandem of PDZ-RhoGEF and C-terminally truncated RhoA were overexpressed in Escherichia coli as TEV protease-cleavable fusion proteins containing GST and a hexahistidine tag at the N-termini, respectively. His-His-His-His-His-His 165-178 ras homolog family member A Homo sapiens 58-62 14551188-3 2003 In an effort to assess its amine oxidase activity, we expressed LOXL4 as recombinant forms attached with hexa-histidine residues at the carboxyl terminus by using an Escherichia coli expression system. His-His-His-His-His-His 105-119 lysyl oxidase like 4 Homo sapiens 64-69 14531843-2 2003 Recently we have demonstrated that replacing His6 by Pro6 in the well-known antagonist SHU-9119 resulted in a potent agonist at the hMC5R (EC50 = 0.072 nm) with full antagonist activity at the hMC3R and the hMC4R. His-His-His-His-His-His 45-49 melanocortin 5 receptor Homo sapiens 132-137 14531843-2 2003 Recently we have demonstrated that replacing His6 by Pro6 in the well-known antagonist SHU-9119 resulted in a potent agonist at the hMC5R (EC50 = 0.072 nm) with full antagonist activity at the hMC3R and the hMC4R. His-His-His-His-His-His 45-49 melanocortin 4 receptor Homo sapiens 207-212 12551944-3 2003 Application of Ni(2+) to the YggB-His(6) channels with the hexahistidine tags added to the ends of their C termini also resulted in a marked but reversible decrease of activities. His-His-His-His-His-His 59-72 hypothetical protein Escherichia coli 29-33 12710997-4 2003 Partially purified full-length estrogen receptor alpha was expressed intracellularly as a C-terminal fusion to a hexa-histidine tag using the baculovirus-expression system. His-His-His-His-His-His 113-127 estrogen receptor 1 Homo sapiens 31-54 11846886-2 2002 RESULTS: We have made recombinant human galectin-1 as a bacterial fusion protein with an N-terminal hexahistidine tag. His-His-His-His-His-His 100-113 galectin 1 Homo sapiens 40-50 12175332-11 2002 Based on these results, we propose that the hexa-histidine tag, in the context of a polypeptide, acts as a divalent cation-dependent nucleation site for chromogranin A aggregation. His-His-His-His-His-His 44-58 chromogranin A Homo sapiens 153-167 12135567-1 2002 A novel human TF-1 cell apoptosis-related protein, TFAR19, cloned from a human leukemia cell line, TF-1, was first overexpressed in Escherichia coli with the sequence Met-Gly-His(6)-Gly-Thr-Asn-Gly, a hexahistidine sequence followed by a hydroxylamine cleavage site attached to its amino terminus. His-His-His-His-His-His 201-214 programmed cell death 5 Homo sapiens 51-57 12455831-0 2002 The overexpressed hexahistidine-tagged human hexokinase type III is inhibited by D-glucose. His-His-His-His-His-His 18-31 hexokinase 3 Homo sapiens 45-64 12323399-4 2002 Fab fragments produced in Escherichia coli were purified by a single step of nickel-chelate affinity chromatography via a hexa-histidine tag. His-His-His-His-His-His 122-136 FA complementation group B Homo sapiens 0-3 12350233-4 2002 RESULTS: VLF-1 was expressed in a recombinant baculovirus as a fusion with both HA and HIS6 tags and its binding activity to different DNA structures was tested. His-His-His-His-His-His 87-91 very late expression factor 1 Autographa californica nucleopolyhedrovirus 9-14 11931641-2 2002 To determine novel binding partners of CSPs, we employed a pull-down strategy from purified rat brain membrane or cytosolic proteins using recombinant hexahistidine-tagged (His(6)-)CSP. His-His-His-His-His-His 151-164 DnaJ heat shock protein family (Hsp40) member C5 Rattus norvegicus 39-42 11922752-3 2002 We describe a method for expressing hexahistidine-tagged human tissue transglutaminase in Escherichia coli BL21(DE3) using the pET-30 Ek/LIC expression vector. His-His-His-His-His-His 36-49 transglutaminase 2 Homo sapiens 63-86 11911461-1 2002 The catalytic and hinge domain (Tyr112-Ile318) of the human membrane type-1 matrix metalloproteinase (MT1-MMP; MMP-14), containing hexa-histidines at the C-terminus (chMT1-MMP), was overexpressed in Escherichia coli. His-His-His-His-His-His 131-146 matrix metallopeptidase 14 Homo sapiens 60-100 11576447-2 2001 The purified complex, which contains Sec61p with an amino terminal hexahistidine tag, was active since it rescued a sec61-3 post-translational translocation defect in a reconstituted system. His-His-His-His-His-His 67-80 translocon subunit SEC61 Saccharomyces cerevisiae S288C 37-43 11570856-0 2001 Expression, purification, and characterization of recombinant nonglycosylated human serum transferrin containing a C-terminal hexahistidine tag. His-His-His-His-His-His 126-139 serotransferrin Mesocricetus auratus 90-101 11856328-1 2002 The nitrilase AtNIT1 from Arabidopsis thaliana was overexpressed in Escherichia coli with an N-terminal His6 tag and purified by zinc chelate affinity chromatography in a single step almost to homogeneity in a 68% yield with a specific activity of 34.1 U.mg-1. His-His-His-His-His-His 104-108 nitrilase 1 Arabidopsis thaliana 14-20 11576447-2 2001 The purified complex, which contains Sec61p with an amino terminal hexahistidine tag, was active since it rescued a sec61-3 post-translational translocation defect in a reconstituted system. His-His-His-His-His-His 67-80 translocon subunit SEC61 Saccharomyces cerevisiae S288C 116-121 11470085-5 2001 Hexahistidine-tagged proteins including recombinant forms of the extracellular regions of murine B7.1 (B7.1-6H) and of the human erythropoietin receptor (EPOR-6H) bind to NTA-DTDA-membranes; the stability of binding is dependent on both protein concentration, and density of NTA-DTDA. His-His-His-His-His-His 0-13 erythropoietin receptor Homo sapiens 129-152 11464505-6 2001 The human UbC promoter was used to drive expression of a hexahistidine-tagged version of human ubiquitin in a variety of mouse tissues from early embryonic stages, as assessed by a green fluorescent protein marker. His-His-His-His-His-His 57-70 ubiquitin C Homo sapiens 10-13 11595173-5 2001 Recombinant hexahistidine tagged MmDjC7 (25 kDa) was efficiently expressed in E. coli and purified to homogeneity. His-His-His-His-His-His 12-25 DnaJ heat shock protein family (Hsp40) member C24 Mus musculus 33-39 11470085-5 2001 Hexahistidine-tagged proteins including recombinant forms of the extracellular regions of murine B7.1 (B7.1-6H) and of the human erythropoietin receptor (EPOR-6H) bind to NTA-DTDA-membranes; the stability of binding is dependent on both protein concentration, and density of NTA-DTDA. His-His-His-His-His-His 0-13 erythropoietin receptor Homo sapiens 154-158 11464505-6 2001 The human UbC promoter was used to drive expression of a hexahistidine-tagged version of human ubiquitin in a variety of mouse tissues from early embryonic stages, as assessed by a green fluorescent protein marker. His-His-His-His-His-His 57-70 ubiquitin Saccharomyces cerevisiae S288C 95-104 11396978-1 2001 An N-terminal hexahistidine-tagged full-length human androgen receptor protein (His(6)-hAR) was overexpressed and purified to apparent homogeneity in the presence of dihydrotestosterone (DHT) in our previous studies. His-His-His-His-His-His 14-27 androgen receptor Homo sapiens 53-70 11396978-1 2001 An N-terminal hexahistidine-tagged full-length human androgen receptor protein (His(6)-hAR) was overexpressed and purified to apparent homogeneity in the presence of dihydrotestosterone (DHT) in our previous studies. His-His-His-His-His-His 14-27 lymphatic vessel endothelial hyaluronan receptor 1 Homo sapiens 87-90 11396977-2 2001 Spodoptera frugiperda (Sf9) cells infected with this virus expressed protein with an N-terminal hexahistidine tag (His(6)-hAR) in soluble and insoluble forms. His-His-His-His-His-His 96-109 lymphatic vessel endothelial hyaluronan receptor 1 Homo sapiens 122-125 11388797-0 2001 One-step purification of a fully active hexahistidine-tagged human hexokinase type I overexpressed in Escherichia coli. His-His-His-His-His-His 40-53 hexokinase 1 Homo sapiens 67-84 11168397-6 2001 Changing the His6 residue of alpha-MSH-ND to Gln or Lys markedly decreased CRE-mediated luciferase activity for MC3R compared with MC4R. His-His-His-His-His-His 13-17 proopiomelanocortin Homo sapiens 29-38 11168397-6 2001 Changing the His6 residue of alpha-MSH-ND to Gln or Lys markedly decreased CRE-mediated luciferase activity for MC3R compared with MC4R. His-His-His-His-His-His 13-17 melanocortin 3 receptor Homo sapiens 112-116 11168397-6 2001 Changing the His6 residue of alpha-MSH-ND to Gln or Lys markedly decreased CRE-mediated luciferase activity for MC3R compared with MC4R. His-His-His-His-His-His 13-17 melanocortin 4 receptor Homo sapiens 131-135 10985779-5 2000 beta(5) monomer could be assembled with partially purified hexahistidine-tagged gamma(2) in vitro to form a functional dimer that could selectively activate PLC beta2 but not PLC beta3. His-His-His-His-His-His 59-72 phospholipase C beta 2 Homo sapiens 157-166 11049752-1 2000 A recombinant hexahistidine-tagged 18.5-kDa isoform of murine myelin basic protein has been characterized biochemically and immunogenically, by mass spectrometry, by circular dichroism under various conditions (in aqueous solution, with monosialoganglioside G(M1), and in 89% 2-propanol), and by transmission electron microscopy. His-His-His-His-His-His 14-27 myelin basic protein Mus musculus 62-82 11182766-4 2001 A plasmid was constructed to express rat IGFBP-4 as a thioredoxin fusion protein that included a hexahistidine sequence to permit affinity purification. His-His-His-His-His-His 97-110 insulin-like growth factor binding protein 4 Rattus norvegicus 41-48 11054422-2 2001 To understand the enzymological properties of the protein, we purified and characterized C-terminal hexahistidine-tagged human CLN2p/tripeptidyl-peptidase I produced from insect cells transfected with a baculovirus vector. His-His-His-His-His-His 100-113 tripeptidyl peptidase 1 Homo sapiens 127-132 11054422-2 2001 To understand the enzymological properties of the protein, we purified and characterized C-terminal hexahistidine-tagged human CLN2p/tripeptidyl-peptidase I produced from insect cells transfected with a baculovirus vector. His-His-His-His-His-His 100-113 tripeptidyl peptidase 1 Homo sapiens 133-156 11099853-4 2000 Solid-phase radioimmunoassay for scFv-His6 showed only 20-25% binding whereas both His6-scFv and scFv (no His-tag) showed 60-65% binding. His-His-His-His-His-His 38-42 immunglobulin heavy chain variable region Homo sapiens 33-37 11099853-7 2000 Comparative homology modeling for scFv and scFv-His6 showed that the C-terminal position of the His-tag partially covered the antigen-binding site of the protein. His-His-His-His-His-His 48-52 immunglobulin heavy chain variable region Homo sapiens 43-47 10662616-2 2000 To facilitate studies on its induction pattern and cellular functions, we expressed recombinant P56 as a hexahistidine-tagged protein in Escherichia coli and purified it to apparent homogeneity using affinity chromatography. His-His-His-His-His-His 105-118 interferon induced protein with tetratricopeptide repeats 1 Homo sapiens 96-99 12483584-1 2000 A fusion protein of hexa-histidine repeat (His) and glycosylphosphatidylinositol (GPI)-anchor region of Saccharomyces cerevisiae Cwp1 with Aspergillus oryzae Taka-amylase A (TAA) was expressed on the yeast cell surface. His-His-His-His-His-His 20-34 Cwp1p Saccharomyces cerevisiae S288C 129-133 10954336-5 2000 Furthermore, by staining the whole oocytes for the hexa-histidine tag attached to the N-terminus of SUR1, we provide direct experimental evidence that the N-terminus of SUR1 is extracellular. His-His-His-His-His-His 51-65 ATP-binding cassette sub-family C member 8 Xenopus laevis 169-173 10769077-2 2000 In this study, an N-terminal hexahistidine-tagged full-length NS3 polypeptide was expressed in Escherichia coli and purified to homogeneity by conventional chromatography. His-His-His-His-His-His 29-42 KRAS proto-oncogene, GTPase Homo sapiens 62-65 10722049-2 2000 We report the expression of hexahistidine-tagged antibody fragments (Fabs) with specificity for Bet v1, the major birch pollen allergen, in Escherichia coli. His-His-His-His-His-His 28-41 putative DNA recombination protein Bet Escherichia coli 96-99 10722049-3 2000 The cDNA coding for the heavy chain fragment of a mouse monoclonal anti-Bet v1 antibody, Bip 1, was engineered by PCR to contain a hexahistidine-encoding 3" end. His-His-His-His-His-His 131-144 putative DNA recombination protein Bet Escherichia coli 72-75 10531346-10 1999 By co-expressing in COS-7 cells and epithelial Caco-2 cells two pIgR constructs tagged at the carboxyl terminus with hexahistidine or FLAG, we provide the strongest evidence reported to date that the pIgR dimerizes noncovalently in the plasma membrane in the absence of polymeric IgA ligand. His-His-His-His-His-His 117-130 polymeric immunoglobulin receptor Homo sapiens 64-68 10968258-10 2000 1995, 38, 4660) using 1-D NOE spectroscopy coupled with the present study of the same type of lead antagonist Sarilesin revealed that the Tyr4-Ile5-His6 bend, a conformational property found in Angiotensin II is not present in type I antagonists. His-His-His-His-His-His 148-152 angiotensinogen Rattus norvegicus 194-208 11035417-2 2000 In this work hexahistidine-tagged recombinant annexin XI (His6- rAnn XI) was used as antigen in ELISA experiments for determination of autoantibodies to annexin XI in sera of patients with systemic rheumatic autoimmune diseases. His-His-His-His-His-His 13-26 annexin A11 Homo sapiens 46-56 11035417-2 2000 In this work hexahistidine-tagged recombinant annexin XI (His6- rAnn XI) was used as antigen in ELISA experiments for determination of autoantibodies to annexin XI in sera of patients with systemic rheumatic autoimmune diseases. His-His-His-His-His-His 13-26 annexin A11 Homo sapiens 153-163 10600464-1 1999 The bacterial expression and purification of human pi class glutathione S-transferase (hGST P1-1) as a hexahistidine-tagged polypeptide was performed. His-His-His-His-His-His 103-116 glutathione S-transferase pi 1 Homo sapiens 87-96 10600464-6 1999 It showed that the real molecular weight of the hexahistidine-tagged hGST P1-1 polypeptide chain agreed with the calculated value and that the purified protein eluted as an apparent homodimer on the gel filtration column. His-His-His-His-His-His 48-61 glutathione S-transferase pi 1 Homo sapiens 69-78 10600464-7 1999 Our expression system allows the expression and purification of active hexahistidine-tagged hGST P1-1 in high yield with no need of removal of the hexahistidine tag and gives pure protein in one purification step allowing further study of this enzyme. His-His-His-His-His-His 71-84 glutathione S-transferase pi 1 Homo sapiens 92-101 10600464-7 1999 Our expression system allows the expression and purification of active hexahistidine-tagged hGST P1-1 in high yield with no need of removal of the hexahistidine tag and gives pure protein in one purification step allowing further study of this enzyme. His-His-His-His-His-His 147-160 glutathione S-transferase pi 1 Homo sapiens 92-101 10531346-10 1999 By co-expressing in COS-7 cells and epithelial Caco-2 cells two pIgR constructs tagged at the carboxyl terminus with hexahistidine or FLAG, we provide the strongest evidence reported to date that the pIgR dimerizes noncovalently in the plasma membrane in the absence of polymeric IgA ligand. His-His-His-His-His-His 117-130 polymeric immunoglobulin receptor Homo sapiens 200-204 10491201-7 1999 Replacement of the His6 residue of alpha-MSH-ND by Gln, Asn, Arg or Lys decreased not only the receptor binding, but also the cAMP-generating activity in both the MC3R and the MC4R. His-His-His-His-His-His 19-23 melanocortin receptor 3 Cricetulus griseus 163-167 10491201-7 1999 Replacement of the His6 residue of alpha-MSH-ND by Gln, Asn, Arg or Lys decreased not only the receptor binding, but also the cAMP-generating activity in both the MC3R and the MC4R. His-His-His-His-His-His 19-23 melanocortin receptor 4 Cricetulus griseus 176-180 10455014-1 1999 Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. His-His-His-His-His-His 177-190 rhodopsin Bos taurus 205-214 10471309-6 1999 Subsequent studies showed that it was plasmin, the product of tPA activation of plasminogen, that specifically cleaved A beta 1-40 in the amino terminal region between Arg5 and His6. His-His-His-His-His-His 177-181 plasminogen Homo sapiens 38-45 10471309-6 1999 Subsequent studies showed that it was plasmin, the product of tPA activation of plasminogen, that specifically cleaved A beta 1-40 in the amino terminal region between Arg5 and His6. His-His-His-His-His-His 177-181 plasminogen activator, tissue type Homo sapiens 62-65 10471309-6 1999 Subsequent studies showed that it was plasmin, the product of tPA activation of plasminogen, that specifically cleaved A beta 1-40 in the amino terminal region between Arg5 and His6. His-His-His-His-His-His 177-181 amyloid beta precursor protein Homo sapiens 119-125 10383892-5 1999 Recombinant rat AKR7A1 and human AKR7A3 were expressed and purified from Escherichia coli as hexa-histidine tagged fusion proteins. His-His-His-His-His-His 93-107 aldo-keto reductase family 7 member A3 Rattus norvegicus 16-22 10425174-2 1999 Here we describe the expression of recombinant human granzyme B in Pichia pastoris as a chimeric zymogen comprising the alpha-factor signal sequence, a prodomain including an enterokinase cleavage site, and the mature granzyme B sequence followed by a hexahistidine tag. His-His-His-His-His-His 252-265 granzyme B Homo sapiens 53-63 10402461-4 1999 Hexahistidine-tagged-GBF1 exhibited BFA-resistant guanine nucleotide exchange activity that appears specific towards ARF5 at physiological Mg2+concentration. His-His-His-His-His-His 0-13 Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 Cricetulus griseus 21-25 10402461-4 1999 Hexahistidine-tagged-GBF1 exhibited BFA-resistant guanine nucleotide exchange activity that appears specific towards ARF5 at physiological Mg2+concentration. His-His-His-His-His-His 0-13 ADP-ribosylation factor 5 Cricetulus griseus 117-121 10383892-5 1999 Recombinant rat AKR7A1 and human AKR7A3 were expressed and purified from Escherichia coli as hexa-histidine tagged fusion proteins. His-His-His-His-His-His 93-107 aldo-keto reductase family 7 member A3 Homo sapiens 33-39 10383764-5 1999 VirB4 also dimerized in Agrobacterium tumefaciens, as demonstrated by the recovery of a detergent-resistant complex of native protein and a functional, histidine-tagged derivative by precipitation with anti-His6 antibodies and by Co2+ affinity chromatography. His-His-His-His-His-His 207-211 type IV secretion system protein VirB4 Agrobacterium tumefaciens 0-5 10336613-4 1999 Immunoprecipitation of F1F0-ATP synthase from mitochondrial lysates prepared from atp20-null cells expressing a variant of subunit g with a hexahistidine motif indicated that this polypeptide was associated with other well-characterized subunits of the yeast complex. His-His-His-His-His-His 140-153 F1F0 ATP synthase subunit g Saccharomyces cerevisiae S288C 82-87 10392722-4 1999 A histidine (His6) tag was introduced close to the C-terminus of the proteinase to aid purification. His-His-His-His-His-His 13-17 endogenous retrovirus group K member 19, envelope Homo sapiens 69-79 10092845-2 1999 The cDNA corresponding to exons 2-4 of the processed human plasminogen (Pgn) gene, encoding the N-terminal peptide domain (NTP), has been cloned, expressed in Escherichia coli as a recombinant protein (r-NTP) containing a hexahistidine tag, and refolded to the native structure that contains two internal cystine bridges. His-His-His-His-His-His 222-235 plasminogen Homo sapiens 59-70 10092845-2 1999 The cDNA corresponding to exons 2-4 of the processed human plasminogen (Pgn) gene, encoding the N-terminal peptide domain (NTP), has been cloned, expressed in Escherichia coli as a recombinant protein (r-NTP) containing a hexahistidine tag, and refolded to the native structure that contains two internal cystine bridges. His-His-His-His-His-His 222-235 plasminogen Homo sapiens 72-75 9850738-4 1998 The baculoviruses expressing full-size Sg-I and Sg-II proteins that were N-terminally-tagged with a hexahistidine were selected, and were infected with Sf 21 cells. His-His-His-His-His-His 100-113 semenogelin 2 Homo sapiens 48-53 9857049-4 1998 We isolated a Chinese hamster ovary cell line that stably expresses the recombinant human ACAT-1 protein bearing an N-terminal hexahistidine tag. His-His-His-His-His-His 127-140 acetyl-CoA acetyltransferase 1 Homo sapiens 90-96 9685327-3 1998 Here, segments S1 and S2 of the NR1 polypeptide were fused via a linker peptide followed by N- and C-terminally tagging with Flag and His6 epitopes, respectively. His-His-His-His-His-His 134-138 glutamate ionotropic receptor NMDA type subunit 1 Rattus norvegicus 32-35 9743625-6 1998 The recombinant mNthl1 with a hexahistidine tag was overexpressed in a nth::cmr nei::Kmr double mutant of E. coli, and purified to apparent homogeneity. His-His-His-His-His-His 30-43 nth (endonuclease III)-like 1 (E.coli) Mus musculus 16-22 9756901-7 1998 PDK1 and PDK2 were expressed in Escherichia coli with N-terminal His6 tags to facilitate purification. His-His-His-His-His-His 65-69 pyruvate, phosphate dikinase 1, chloroplastic Zea mays 0-4 9756901-7 1998 PDK1 and PDK2 were expressed in Escherichia coli with N-terminal His6 tags to facilitate purification. His-His-His-His-His-His 65-69 pyruvate dehydrogenase (lipoamide) kinase 2 Zea mays 9-13 9699465-1 1998 Results of energy calculations for alpha-MSH (alpha-melanocyte stimulating hormone, Ac-Ser1-Tyr2-Ser3-Met4-Glu5-His6-Phe7-Arg8-Trp9- Gly10-Lys11-Pro12-Val13-NH2) and [D-Phe7] alpha-MSH were used for design of cyclic peptides with the general aim to stabilize different conformational isomers of the parent compound. His-His-His-His-His-His 112-116 proopiomelanocortin Homo sapiens 35-44 9675063-5 1998 To understand the structure and function of human RNA polymerases, we overexpressed the common subunits hRPABC25, hRPABC17, and hRPABC14.4 as hexahistidine fusions in Escherichia coli. His-His-His-His-His-His 142-155 RNA polymerase II, I and III subunit F Homo sapiens 128-138 9521779-2 1998 Mdr3, tagged with a hexahistidine tail, was overexpressed in the yeast Pichia pastoris and purified to about 90% homogeneity by Ni-affinity chromatography. His-His-His-His-His-His 20-33 ATP-binding cassette, sub-family B (MDR/TAP), member 1A Mus musculus 0-4 9744743-3 1998 This was accomplished by engineering a hexahistidine tag on the C-terminus of the CD40 and binding, in a uniform orientation, up to 100 molecules of hexahistidine CD40 by metal chelation to a single fluorescently tagged dextran molecule. His-His-His-His-His-His 39-52 CD40 antigen Mus musculus 82-86 9744743-3 1998 This was accomplished by engineering a hexahistidine tag on the C-terminus of the CD40 and binding, in a uniform orientation, up to 100 molecules of hexahistidine CD40 by metal chelation to a single fluorescently tagged dextran molecule. His-His-His-His-His-His 39-52 CD40 antigen Mus musculus 163-167 9744743-3 1998 This was accomplished by engineering a hexahistidine tag on the C-terminus of the CD40 and binding, in a uniform orientation, up to 100 molecules of hexahistidine CD40 by metal chelation to a single fluorescently tagged dextran molecule. His-His-His-His-His-His 149-162 CD40 antigen Mus musculus 82-86 9744743-3 1998 This was accomplished by engineering a hexahistidine tag on the C-terminus of the CD40 and binding, in a uniform orientation, up to 100 molecules of hexahistidine CD40 by metal chelation to a single fluorescently tagged dextran molecule. His-His-His-His-His-His 149-162 CD40 antigen Mus musculus 163-167 9744743-11 1998 that the A20 cells have a counterstructure for CD40, and propose that the high binding of CD40 observed in this study may have been due to an exposed hexahistidine tag on the molecule. His-His-His-His-His-His 150-163 CD40 antigen Mus musculus 90-94 9535798-5 1998 The presence of a hexahistidine tag on ATM was used as the basis for purification of the protein by affinity chromatography. His-His-His-His-His-His 18-31 ATM serine/threonine kinase Homo sapiens 39-42 9478938-2 1998 Either a hexahistidine or a FLAG octapeptide tag was incorporated into the FAS at either the amino terminus, within an internal noncatalytic domain, or at the carboxyl terminus. His-His-His-His-His-His 9-22 fatty acid synthase Homo sapiens 75-78 9525272-2 1998 To improve the analysis of CYP1 proteins, specific CYP1B1 and CYP1A1 polypeptides were expressed as hexahistidine-tagged fusion proteins in Escherichia coli, purified to homogeneity and used to produce polyclonal antibodies in rabbits. His-His-His-His-His-His 100-113 cytochrome P450, family 1, subfamily b, polypeptide 1 Rattus norvegicus 51-57 9525272-2 1998 To improve the analysis of CYP1 proteins, specific CYP1B1 and CYP1A1 polypeptides were expressed as hexahistidine-tagged fusion proteins in Escherichia coli, purified to homogeneity and used to produce polyclonal antibodies in rabbits. His-His-His-His-His-His 100-113 cytochrome P450, family 1, subfamily a, polypeptide 1 Rattus norvegicus 62-68 9611778-4 1998 Purification of recombinant transketolase and separation from the E. coli enzyme has been greatly simplified by means of a non-cleavable hexa-histidine tag. His-His-His-His-His-His 137-151 transketolase Homo sapiens 28-41 9398350-2 1997 0K-beta2 has the following modifications: (1) all 16 lysines in the wild-type beta2 receptor were mutated to arginines, (2) a FLAG epitope preceded by a cleaved hemagglutinin signal sequence was fused to the amino terminus, and (3) a hexahistidine tail was added to the carboxyl terminus. His-His-His-His-His-His 234-247 potassium calcium-activated channel subfamily M regulatory beta subunit 2 Homo sapiens 3-8 9492319-3 1998 Purification of the denatured fusion protein was simplified greatly by the introduction of a C-terminal histidine anchor, leading to 255 mg pure GST-PrPc-His6/l bacterial broth, which could be refolded easily by dilution in 20 mM Tris, 5 mM dithiothreitol, 1 mM EDTA, pH 9.0. His-His-His-His-His-His 154-158 glutathione S-transferase Mesocricetus auratus 145-148 9490615-1 1997 The N-terminal fragment of ATP1AL1, the possible catalytic subunit of human ouabain-sensitive H+,K(+)-ATPase, was expressed in Escherichia coli cells as two recombinant proteins: the Ser14-Ile104 fragment or the same fragment containing His6 sequence at its N-end. His-His-His-His-His-His 237-241 ATPase H+/K+ transporting non-gastric alpha2 subunit Homo sapiens 27-34 9089659-4 1997 Our new data of His6 and Phe7 substituted MSH peptides are compared with previous results and the hypothesis of putative interactions of D117 and H260 with single amino acids in the MSH peptide. His-His-His-His-His-His 16-20 proopiomelanocortin Homo sapiens 182-185 9299349-3 1997 When the alpha-subunit (alpha-MPP) was co-expressed with N-terminal hexa-histidine tagged beta-MPP, alpha-MPP was co-eluted from a nickel-derivatized affinity resin, with a 1:1 stochiometry, both with wild-type beta-MPP and with the mutants with alanine inserted after Ser114 and Ser314. His-His-His-His-His-His 68-82 peptidase, mitochondrial processing subunit alpha Homo sapiens 24-33 9299349-3 1997 When the alpha-subunit (alpha-MPP) was co-expressed with N-terminal hexa-histidine tagged beta-MPP, alpha-MPP was co-eluted from a nickel-derivatized affinity resin, with a 1:1 stochiometry, both with wild-type beta-MPP and with the mutants with alanine inserted after Ser114 and Ser314. His-His-His-His-His-His 68-82 peptidase, mitochondrial processing subunit beta Homo sapiens 90-98 9299349-3 1997 When the alpha-subunit (alpha-MPP) was co-expressed with N-terminal hexa-histidine tagged beta-MPP, alpha-MPP was co-eluted from a nickel-derivatized affinity resin, with a 1:1 stochiometry, both with wild-type beta-MPP and with the mutants with alanine inserted after Ser114 and Ser314. His-His-His-His-His-His 68-82 peptidase, mitochondrial processing subunit alpha Homo sapiens 100-109 9126381-0 1997 The use of Ni-nitrilotriacetic acid agarose for estimation of affinities of hexahistidine-tagged Fab to single-stranded DNA. His-His-His-His-His-His 76-89 FA complementation group B Homo sapiens 97-100 9126381-1 1997 The complex formed between 32P-labeled (dT)15 and a hexahistidine (6-His)-tagged anti-single-stranded DNA (ssDNA) Fab, DNA-1, was trapped by addition of nickel-chelating nitrilotriacetic acid (Ni-NTA) agarose that led to efficient separation of bound ligand from free. His-His-His-His-His-His 52-65 FA complementation group B Homo sapiens 114-117 9116497-4 1997 Expression at 25 degrees C eliminated formation of insoluble SRP19 and resulted in accumulation of soluble hexahistidine-SRP19 to 68% of total cell protein after 24 h. Metal chelation chromatography yielded 40 mg of hexahistidine-SRP19 per liter of culture, with a purity slightly greater than 97%. His-His-His-His-His-His 107-120 signal recognition particle 19 Homo sapiens 121-126 9061798-1 1997 A 124-residue N-terminal fragment corresponding to the DNA-binding domain of the Saccharomyces cerevisae cell-cycle transcription factor MBP-1 has been expressed with a hexahistidine affinity tag in E. coli and purified to apparent homogeneity. His-His-His-His-His-His 169-182 transcription factor MBP1 Saccharomyces cerevisiae S288C 137-142 9116497-4 1997 Expression at 25 degrees C eliminated formation of insoluble SRP19 and resulted in accumulation of soluble hexahistidine-SRP19 to 68% of total cell protein after 24 h. Metal chelation chromatography yielded 40 mg of hexahistidine-SRP19 per liter of culture, with a purity slightly greater than 97%. His-His-His-His-His-His 107-120 signal recognition particle 19 Homo sapiens 121-126 9116497-4 1997 Expression at 25 degrees C eliminated formation of insoluble SRP19 and resulted in accumulation of soluble hexahistidine-SRP19 to 68% of total cell protein after 24 h. Metal chelation chromatography yielded 40 mg of hexahistidine-SRP19 per liter of culture, with a purity slightly greater than 97%. His-His-His-His-His-His 216-229 signal recognition particle 19 Homo sapiens 121-126 9116497-4 1997 Expression at 25 degrees C eliminated formation of insoluble SRP19 and resulted in accumulation of soluble hexahistidine-SRP19 to 68% of total cell protein after 24 h. Metal chelation chromatography yielded 40 mg of hexahistidine-SRP19 per liter of culture, with a purity slightly greater than 97%. His-His-His-His-His-His 216-229 signal recognition particle 19 Homo sapiens 121-126 9116497-7 1997 The purified protein was also photochemically crosslinked to the 150-nucleotide SRP RNA fragment, providing the means to potentially identify portions of hexahistidine-SRP19 which are in close proximity to the RNA molecule. His-His-His-His-His-His 154-167 signal recognition particle 19 Homo sapiens 168-173 8910377-4 1996 To test the interaction of PI-9 with granzyme B we prepared recombinant hexa-histidine tagged PI-9 in a yeast expression system. His-His-His-His-His-His 72-86 serpin family B member 9 Homo sapiens 94-98 8806762-3 1996 The gene for the neutral endopeptidase from L. lactis was cloned into the pQE expression vector, resulting in the fusion of a hexahistidine at the N-terminus. His-His-His-His-His-His 126-139 pepO Lactococcus lactis 17-38 8862403-3 1996 Core proteins with amino-terminal hexa-histidine tags were expressed which allowed the purification of the hexa-histidine P19 core with NI(2+)-NTA columns. His-His-His-His-His-His 34-48 interleukin 23 subunit alpha Homo sapiens 122-125 8567900-5 1995 Open reading frames (ORF) 2 and 3 were expressed in Escherichia coli as N-terminal hexahistidine epitope fusions. His-His-His-His-His-His 83-96 hypothetical protein Escherichia coli 0-33 8755506-1 1996 The X and Y domains of phospholipase C (PLC)-gamma1, which are conserved in all mammalian phosphoinositide-specific PLC isoforms and are proposed to interact to form the catalytic site, have been expressed as individual hexahistidine-tagged fusion proteins in the baculovirus system. His-His-His-His-His-His 220-233 phospholipase C gamma 1 Homo sapiens 23-51 8647121-2 1996 Recombinant yeast ATF, modified and extended by an amino-terminal in-frame insertion of a His6 tract, was purified from total protein extracts by nickel chelate affinity chromatography and shown to be functionally active since it efficiently competes with uPA for binding to cell-surface-associated uPAR. His-His-His-His-His-His 90-94 glial cell derived neurotrophic factor Homo sapiens 18-21 8647124-4 1996 In a second construct an additional hexa-histidine tag was introduced at the C-terminus of the CCKB receptor to enable employment of metal affinity chromatography. His-His-His-His-His-His 36-50 cholecystokinin B receptor Homo sapiens 95-99 8631807-4 1996 Moreover, we have expressed soluble mouse Rt6-1 and Rt6-2 gene products in which C-terminal tags (FLAG-His6) replace the native glycosylphosphatidylinositol anchor signal sequences. His-His-His-His-His-His 103-107 ADP-ribosyltransferase 2a Mus musculus 42-57 9630891-3 1996 A hexahistidine tag has been incorporated to allow rapid purification of scFv by nickel chelate chromatography. His-His-His-His-His-His 2-15 immunglobulin heavy chain variable region Homo sapiens 73-77 7592834-8 1995 Incubation of synthetic A beta 1-40 peptide with increasing concentrations of FXIa resulted in cleavage of A beta between Arg5 and His6 within the cell adhesion domain of the peptide. His-His-His-His-His-His 131-135 amyloid beta precursor protein Homo sapiens 24-30 7592834-8 1995 Incubation of synthetic A beta 1-40 peptide with increasing concentrations of FXIa resulted in cleavage of A beta between Arg5 and His6 within the cell adhesion domain of the peptide. His-His-His-His-His-His 131-135 amyloid beta precursor protein Homo sapiens 107-113 7628706-1 1995 To facilitate the purification and conjugation of single-chain antibodies (scFv) selected from a phage display library, we have incorporated His6, an amber stop codon and a C-terminal Cys into a surface expression vector. His-His-His-His-His-His 141-145 immunglobulin heavy chain variable region Homo sapiens 75-79 7972003-3 1994 A hexahistidine tag was inserted in the N-terminal region of histone H2B, permitting resolution of the histone octamer from high-salt extracts of nuclei or chromatin to near homogeneity. His-His-His-His-His-His 2-15 histone H2B Saccharomyces cerevisiae S288C 61-72 7539431-1 1995 Active, recombinant p68 reverse transcriptase (RT) from human immunodeficiency virus type 2 (HIV-2), with an NH2-terminal extension containing a hexahistidine sequence was isolated from extracts of Escherichia coli by immobilized metal affinity chromatography. His-His-His-His-His-His 145-158 GATA zinc finger domain containing 2B Homo sapiens 20-23 7765987-2 1995 A second derivative of factor Xa in which a hexahistidine tail is fused to the C-terminus of the heavy chain (FXa-H6) also retains activity when immobilized, in this case on Ni(2+)-NTA agarose. His-His-His-His-His-His 44-57 coagulation factor X Homo sapiens 23-32 7961908-4 1994 His6-NSF possessed ATPase activity, which was enhanced in a dose-dependent manner by immobilized (i.e. plastic-adsorbed) but not soluble His6-tagged SNAPs. His-His-His-His-His-His 0-4 N-ethylmaleimide sensitive factor, vesicle fusing ATPase Homo sapiens 5-8 7961908-4 1994 His6-NSF possessed ATPase activity, which was enhanced in a dose-dependent manner by immobilized (i.e. plastic-adsorbed) but not soluble His6-tagged SNAPs. His-His-His-His-His-His 0-4 dynein axonemal heavy chain 8 Homo sapiens 19-25 7980570-1 1994 The murine C-5 cytosine DNA methyltransferase (MTase, E.C.2.1.1.37) containing a hexahistidine affinity leader peptide has been expressed at levels which are at least 50-fold higher than previously reported. His-His-His-His-His-His 81-94 hemolytic complement Mus musculus 11-14 7980570-1 1994 The murine C-5 cytosine DNA methyltransferase (MTase, E.C.2.1.1.37) containing a hexahistidine affinity leader peptide has been expressed at levels which are at least 50-fold higher than previously reported. His-His-His-His-His-His 81-94 DNA methyltransferase (cytosine-5) 1 Mus musculus 47-52 7833034-3 1994 A hexahistidine sequence fused to the N terminus of Gal4 allows efficient purification of the bacterially produced protein by affinity chromatography on nickel nitrilotriacetic acid (Ni-NTA) columns. His-His-His-His-His-His 2-15 galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C 52-56 7833034-0 1994 A vector, pHisGal, allowing bacterial production of proteins fused to a hexahistidine-tagged Gal4 DNA-binding domain. His-His-His-His-His-His 72-85 galactose-responsive transcription factor GAL4 Saccharomyces cerevisiae S288C 93-97 7926798-5 1994 An in-frame fusion was made such that a His6 stretch was produced at the C terminus of SP-10. His-His-His-His-His-His 40-44 acrosomal vesicle protein 1 Homo sapiens 87-92 7985780-6 1994 The assay relied on the use of hexahistidine-tagged recombinant Neurospora 21-kDa eIF-5A precursor protein as the substrate protein. His-His-His-His-His-His 31-44 eukaryotic translation initiation factor 5A Homo sapiens 82-88 8503891-4 1993 The hexahistidine tail could be removed when desired by digestion with Factor Xa. His-His-His-His-His-His 4-17 coagulation factor X Homo sapiens 71-80 8142357-1 1994 Conformations of two cyclic analogs of angiotensin (Asp1-Arg2-Val3-Tyr4-Val/Ile5-His6-Pro7-Phe8, AT), cyclo[Sar1, Cys3, Mpt5]-AT and cyclo[Sar1, HCys3, Mpt5]-AT, were studied, independently employing two complementary techniques, energy calculations and NMR measurements in DMSO solution. His-His-His-His-His-His 81-85 beta-secretase 2 Homo sapiens 52-56 8307012-6 1994 The hexahistidine tail was successfully removed with FXa. His-His-His-His-His-His 4-17 coagulation factor X Homo sapiens 53-56 8229004-7 1993 Amino acid sequencing reveals A beta species with N-termini of Asp1, Glu3, His6, Glu11, and Val12, although on a molar basis, Asp1 represents the predominant aminoterminus. His-His-His-His-His-His 75-79 amyloid beta precursor protein Homo sapiens 30-36 8229004-8 1993 Laser desorption mass spectrometry confirmed the presence in CSF of A beta species containing 27, 28, 30, 34, 35, 40, 42, and 43 amino acids, all beginning at Asp1; two stable trimers, (Asp1-Met35)3 and (His6-Ala42)3; and one stable dimer containing (Asp1-Val40)2. His-His-His-His-His-His 204-208 amyloid beta precursor protein Homo sapiens 68-74 8223584-7 1993 Recombinant IL-1 beta was expressed in Escherichia coli as a fusion protein containing an N-terminal hexahistidine tag followed by a factor-Xa-cleavage site. His-His-His-His-His-His 101-114 interleukin 1 beta Bos taurus 12-21 7504537-1 1993 The human TATA-binding protein was expressed in Escherichia coli as a fusion with an N-terminal hexahistidine sequence, partially purified, and used to raise monoclonal antibodies. His-His-His-His-His-His 96-109 TATA-box binding protein Homo sapiens 10-30 24594699-6 2014 By contrast, hexahistidine-tagged hGCSF was insoluble at both temperatures. His-His-His-His-His-His 13-26 colony stimulating factor 3 Homo sapiens 34-39 1581636-6 1992 The working model of AII, which has a twisted U-shape, places the imidazole of His6 and the terminal carboxylate approximately 8.7 A apart. His-His-His-His-His-His 79-83 angiotensinogen Homo sapiens 21-24 2241171-5 1990 Amino-terminal sequence analysis indicated that AGT-1 and AGT-2 were modified at the His9 and the His6, respectively. His-His-His-His-His-His 98-102 solute carrier family 7 member 13 Homo sapiens 48-53 2241171-5 1990 Amino-terminal sequence analysis indicated that AGT-1 and AGT-2 were modified at the His9 and the His6, respectively. His-His-His-His-His-His 98-102 alanine--glyoxylate aminotransferase 2 Homo sapiens 58-63 34325320-4 2021 SULT1E1 was site-specifically immobilized on transparent glass slides via a hexahistidine-tag in a multi-step procedure. His-His-His-His-His-His 76-89 sulfotransferase family 1E member 1 Homo sapiens 0-7 34709522-4 2021 In the current study, we used a pET32a vector with an N-terminal Trx-His6 tag to express Myo9a-RhoGAP in a soluble form. His-His-His-His-His-His 69-73 thioredoxin Homo sapiens 65-68 34709522-4 2021 In the current study, we used a pET32a vector with an N-terminal Trx-His6 tag to express Myo9a-RhoGAP in a soluble form. His-His-His-His-His-His 69-73 myosin IXA Homo sapiens 89-94 34709522-4 2021 In the current study, we used a pET32a vector with an N-terminal Trx-His6 tag to express Myo9a-RhoGAP in a soluble form. His-His-His-His-His-His 69-73 Rho GTPase activating protein 1 Homo sapiens 95-101 34699194-7 2021 Moreover, Ca(II) binding causes local and allosteric ordering of the His3Asp and His6 metal-binding sites. His-His-His-His-His-His 81-85 carbonic anhydrase 2 Homo sapiens 10-16 34699194-9 2021 Remarkably, Ca(II) binding causes allosteric effects in the C-terminal region of helix alphaIV of S100A9, which stabilize the alpha-helicity at positions H91 and H95 and thereby organize the functionally versatile His6 site. His-His-His-His-His-His 214-218 carbonic anhydrase 2 Homo sapiens 12-18 34699194-9 2021 Remarkably, Ca(II) binding causes allosteric effects in the C-terminal region of helix alphaIV of S100A9, which stabilize the alpha-helicity at positions H91 and H95 and thereby organize the functionally versatile His6 site. His-His-His-His-His-His 214-218 S100 calcium binding protein A9 Homo sapiens 98-104 34391728-5 2021 Important features of the hNit2 gene within the pQE-Nit22 construct are: 1) optimized codon composition, 2) the presence of an N-terminus His6 tag immediately after the initiating codon ATG (Met) that permits efficient purification of the end-product on a Ni-NTA-agarose column. His-His-His-His-His-His 138-142 nitrilase family member 2 Homo sapiens 26-31 34343891-14 2021 The other residues such as Glu203, Ser537, Gly140, Val587, and Val536 could also play an important role in the cleavage of Abeta peptide in between Asp1-Ala2, Arg5-His6, Val18-Phe19, Gly9-Tyr10, and Arg5-His6. His-His-His-His-His-His 164-168 amyloid beta precursor protein Homo sapiens 123-128 34293440-3 2021 The repeat region of Pmel17 (RPT, residues 315-444) has been previously shown to form amyloid aggregates under acidic melanosomal conditions, but not under neutral cytosolic conditions, when expressed and purified using a C-terminal hexa-histidine tag (RPT-His). His-His-His-His-His-His 233-247 premelanosome protein Homo sapiens 21-27 34343891-14 2021 The other residues such as Glu203, Ser537, Gly140, Val587, and Val536 could also play an important role in the cleavage of Abeta peptide in between Asp1-Ala2, Arg5-His6, Val18-Phe19, Gly9-Tyr10, and Arg5-His6. His-His-His-His-His-His 204-208 amyloid beta precursor protein Homo sapiens 123-128 35098395-0 2022 Modified uvsY by N-terminal hexahistidine tag addition enhances efficiency of recombinase polymerase amplification to detect SARS-CoV-2 DNA. His-His-His-His-His-His 28-41 recombination mediator protein UvsY Escherichia phage T4 9-13 34067755-5 2021 In the five E. coli strains BL21 (DE3), ClearColi BL21 (DE3), LOBSTR, SHuffle T7 and Origami2 (DE3), the hexahistidine-tagged hGM-CSF showed the best expression but was insoluble in all cases at each examined temperature. His-His-His-His-His-His 105-118 colony stimulating factor 2 Homo sapiens 126-133 34792001-10 2021 The C-terminal tagged NEK7-His6 had a much higher purity than that of an N-terminal tag. His-His-His-His-His-His 27-31 NIMA related kinase 7 Homo sapiens 22-26 35412800-2 2022 This array was fabricated as multiplex sensing probes by functionalizing magnetic beads with MMP-specific peptide-isobaric tags for relative and absolute quantification (iTRAQ) conjugates, which contained a hexahistidine tag for surface binding, a substrate region for MMP cleavage, and a coding region for the specific MMP. His-His-His-His-His-His 207-220 matrix metallopeptidase 2 Homo sapiens 93-96 33375972-0 2021 A fast and easy strategy for lytic polysaccharide monooxygenase-cleavable His6-Tag cloning, expression, and purification. His-His-His-His-His-His 74-78 long intergenic non-protein coding RNA 1194 Homo sapiens 79-82 2668272-2 1989 These studies show that the side chains of Arg2, His6, Asn8, and Arg10 are critical for high affinity binding to operator DNA. His-His-His-His-His-His 49-53 arginase 2 Homo sapiens 43-47 6332195-4 1984 Ac-[Cys4,Cys10]-alpha-MSH4-10-NH2 (VI), which constitutes the cyclic analogue of the putative active site sequence -Met4-Glu5-His6-Phe7-Arg8-Trp9-Gly10- of alpha-MSH, was much less active. His-His-His-His-His-His 126-130 pro-opiomelanocortin-alpha Mus musculus 16-25 7050665-8 1982 The sst1 gene was mapped to within 6.9 centimorgans of his6 on chromosome IX. His-His-His-His-His-His 55-59 aspartyl protease BAR1 Saccharomyces cerevisiae S288C 4-8 35328348-2 2022 The first 14 amino acid sequence of the Ctr1 ectodomain Ctr1(1-14) encompasses the characteristic Amino Terminal Cu2+ and Ni2+ binding motif (ATCUN) as well as the bis-His binding motif (His5 and His6). His-His-His-His-His-His 196-200 calcitonin receptor Homo sapiens 40-44 3729426-3 1986 Comparison of the chemical shifts for the His6 and Phe8 ring protons in these peptides suggested a His/Phe stacking interaction in [Sar1,Sar7]Ang II which is important for agonist activity. His-His-His-His-His-His 42-46 secretion associated Ras related GTPase 1A Homo sapiens 132-148 6380499-1 1984 Linear and cyclic peptides containing the His6-Pro7-Phe8-His9 sequence of renin"s substrate (angiotensinogen) have been shown to be effective competitive inhibitors of the enzyme. His-His-His-His-His-His 42-46 renin Homo sapiens 74-79 6380499-1 1984 Linear and cyclic peptides containing the His6-Pro7-Phe8-His9 sequence of renin"s substrate (angiotensinogen) have been shown to be effective competitive inhibitors of the enzyme. His-His-His-His-His-His 42-46 angiotensinogen Homo sapiens 93-108 924383-6 1977 According to the above-mentioned molecular weight and to the above-mentioned molecular weight and to the quantitative amino acid analysis after total hydrolysis, the following compositon of the enzymes subunit was calculated ASX23-25 Thr7 Ser23-24 Glx29-31 Pro22-23 Gly22-24 Ala36-37 Val23-26 Met7 Ile9 Leu34-35 Tyr10 Phe11-12 Lys11-12 Cys6-7 His6-8 Arg22 Trp1-2. His-His-His-His-His-His 343-347 transient receptor potential cation channel subfamily C member 1 Bos taurus 356-360 34048525-2 2021 Herein, by encapsulating a protein (catalase, an enzyme) in a hexahistidine-metal assembly (HmA) via a de novo strategy under mild conditions, we demonstrated that HmA could maintain the bioactivity of the enzyme, protect the enzyme from proteinase degradation, and deliver the encapsulated protein for the prevention of disease in an acute liver injury model. His-His-His-His-His-His 62-75 catalase Homo sapiens 36-44 34048525-2 2021 Herein, by encapsulating a protein (catalase, an enzyme) in a hexahistidine-metal assembly (HmA) via a de novo strategy under mild conditions, we demonstrated that HmA could maintain the bioactivity of the enzyme, protect the enzyme from proteinase degradation, and deliver the encapsulated protein for the prevention of disease in an acute liver injury model. His-His-His-His-His-His 62-75 endogenous retrovirus group K member 25 Homo sapiens 238-248 33375972-9 2021 Moreover, His6-tagged LPMO constructs can be submitted to TEV proteolysis for removal of the questionable C-terminal His6-Tag, obtaining a close-to-native form of LPMO. His-His-His-His-His-His 10-14 long intergenic non-protein coding RNA 1194 Homo sapiens 122-125 33385272-3 2021 Compared to the hexa-histidine tagged S. aureus enolase (rSaeno), the deletion mutant showed the negligible difference in Km, but approximately 20-25% reduction in maximum reaction velocity (Vmax) and 2% reduction in turnover number were observed. His-His-His-His-His-His 16-30 AT695_RS13760 Staphylococcus aureus 48-55