PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 34928609-4 2021 We substituted the Mg(II) cofactor at the ATPase site with paramagnetic Mn(II) and established the binding of Sba1 by measuring the distance between Mn(II) and a nitroxide (NO) spin-label on Sba1. Hydroxylamine 162-171 Hsp90 cochaperone SBA1 Saccharomyces cerevisiae S288C 110-114 34928609-4 2021 We substituted the Mg(II) cofactor at the ATPase site with paramagnetic Mn(II) and established the binding of Sba1 by measuring the distance between Mn(II) and a nitroxide (NO) spin-label on Sba1. Hydroxylamine 162-171 Hsp90 cochaperone SBA1 Saccharomyces cerevisiae S288C 191-195 34897839-6 2022 Aldehyde-modified cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is used as a chimeric antigen receptor (CAR) targeting group to modify the surface of macrophages by aldehyde/hydroxylamine condensation to precisely target central M1-type microglia (CAR-M-UZPM). Hydroxylamine 181-194 cytotoxic T-lymphocyte associated protein 4 Homo sapiens 18-61 34897839-6 2022 Aldehyde-modified cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is used as a chimeric antigen receptor (CAR) targeting group to modify the surface of macrophages by aldehyde/hydroxylamine condensation to precisely target central M1-type microglia (CAR-M-UZPM). Hydroxylamine 181-194 cytotoxic T-lymphocyte associated protein 4 Homo sapiens 63-69 34897839-6 2022 Aldehyde-modified cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is used as a chimeric antigen receptor (CAR) targeting group to modify the surface of macrophages by aldehyde/hydroxylamine condensation to precisely target central M1-type microglia (CAR-M-UZPM). Hydroxylamine 181-194 nuclear receptor subfamily 1 group I member 3 Homo sapiens 84-109 34897839-6 2022 Aldehyde-modified cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is used as a chimeric antigen receptor (CAR) targeting group to modify the surface of macrophages by aldehyde/hydroxylamine condensation to precisely target central M1-type microglia (CAR-M-UZPM). Hydroxylamine 181-194 nuclear receptor subfamily 1 group I member 3 Homo sapiens 111-114 34897839-6 2022 Aldehyde-modified cytotoxic T-lymphocyte-associated protein-4 (CTLA-4) is used as a chimeric antigen receptor (CAR) targeting group to modify the surface of macrophages by aldehyde/hydroxylamine condensation to precisely target central M1-type microglia (CAR-M-UZPM). Hydroxylamine 181-194 nuclear receptor subfamily 1 group I member 3 Homo sapiens 255-258 34943874-5 2021 In contrast, the very low levels of Ngb (~1 muM) in most tissues and organs support (pseudo-)enzymatic properties including NO/O2 metabolism, peroxynitrite and free radical scavenging, nitrite, hydroxylamine, hydrogen sulfide reduction, and the nitration of aromatic compounds. Hydroxylamine 194-207 neuroglobin Mus musculus 36-39 34224197-3 2021 The hydroxamate group is liable to metabolically generate mutagenetic hydroxylamine; therefore, non-hydroxamate HDAC6 inhibitors would be advantageous. Hydroxylamine 70-83 histone deacetylase 6 Homo sapiens 112-117 34649176-5 2021 Even modest values of J are large relative to the separation between trityl and nitroxide resonances for some nitrogen nuclear spin state. Hydroxylamine 80-89 spindlin 1 Homo sapiens 127-131 34771067-4 2021 In the present study, a series of syn and anti isomers of N-substituted indole-3-carbaldehyde oxime derivatives was synthesized via Schiff base reaction of appropriate carbaldehyde derivatives with hydroxylamine hydrochloride. Hydroxylamine 198-225 synemin Homo sapiens 34-37 34609135-6 2021 Nitroxide spin-label probes were covalently attached to particular cysteine residues engineered in GCAP5: C15, C17, T26C, C28, N56C, C69, C105, N139C, E152C, and S159C. Hydroxylamine 0-9 guanylate cyclase activator 1e Danio rerio 99-104 34546243-2 2021 This method utilizes dual paramagnetically-labeled probes consisting of a nitroxide spin probe and a Gd3+ ion flanking a peptide that could be specifically cleaved by protease caspase-3. Hydroxylamine 74-83 caspase 3 Homo sapiens 176-185 34399903-3 2021 Based on chemoselective formation of oximes by solid phase immobilized hydroxylamine derivatives we proposed the protocol for derivatization and selective detection of carbonylated compounds in human serum albumin hydrolysate as a complex peptide mixture and of testosterone in urine samples. Hydroxylamine 71-84 albumin Homo sapiens 200-213 34083449-4 2021 Oxidation of the clusters by the stable nitroxide TEMPOL caused their disassembly, potently inhibited the RdRp, and blocked SARS-CoV-2 replication in cell culture. Hydroxylamine 40-49 ORF1a polyprotein;ORF1ab polyprotein Severe acute respiratory syndrome coronavirus 2 106-110 34085520-7 2021 Nitroxides effectively inhibited the consumption of MPO"s substrate hydrogen peroxide (H2O2) and formation of HOCl catalyzed by endothelial-localized MPO, with their efficacy dependent on both nitroxide and conjugated-polyamine structure. Hydroxylamine 193-202 myeloperoxidase Homo sapiens 52-55 34085520-7 2021 Nitroxides effectively inhibited the consumption of MPO"s substrate hydrogen peroxide (H2O2) and formation of HOCl catalyzed by endothelial-localized MPO, with their efficacy dependent on both nitroxide and conjugated-polyamine structure. Hydroxylamine 193-202 myeloperoxidase Homo sapiens 150-153 35431540-2 2022 Methods: A series of novel CDK4/HDACs inhibitors were designed and synthesized by incorporating the HDAC pharmacophores (hydroxylamine or o-diaminoaniline) into the basic structure of our newly obtained 2-anilino-4-triazolpyrimidine based CDK4 inhibitors. Hydroxylamine 121-134 cyclin dependent kinase 4 Homo sapiens 27-31 35431540-2 2022 Methods: A series of novel CDK4/HDACs inhibitors were designed and synthesized by incorporating the HDAC pharmacophores (hydroxylamine or o-diaminoaniline) into the basic structure of our newly obtained 2-anilino-4-triazolpyrimidine based CDK4 inhibitors. Hydroxylamine 121-134 cyclin dependent kinase 4 Homo sapiens 239-243 35352981-3 2022 To advance understanding on DNA unwinding by Cas9, we combined two types of spectroscopic label, 2-aminopurine and nitroxide spin-label, to investigate unwinding at a specific DNA base pair induced by Streptococcus pyogenes Cas9. Hydroxylamine 115-124 type II CRISPR RNA-guided endonuclease Cas9 Streptococcus pyogenes 45-49 35128864-12 2022 The immunoactivity (IA) of heme oxygenase 1 (HO-1) in the liver was detected by immunohistochemistry, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) of the liver were measured by hydroxylamine method for assessing the level of oxidative stress. Hydroxylamine 220-233 heme oxygenase 1 Mus musculus 27-43 35226325-5 2022 Highly structured, several hundred nucleotides long RNAs with two nitroxide spin labels at specific positions can be prepared by this method. Hydroxylamine 66-75 spindlin 1 Homo sapiens 76-80 35509368-2 2022 Here, we consider the case of two methyl groups bound to the same sp 3 -hybridized atom, which is important in the context of common nitroxide spin labels. Hydroxylamine 135-144 Sp3 transcription factor Homo sapiens 66-71 35509368-2 2022 Here, we consider the case of two methyl groups bound to the same sp 3 -hybridized atom, which is important in the context of common nitroxide spin labels. Hydroxylamine 135-144 spindlin 1 Homo sapiens 145-149 3210953-1 1988 The positively charged nitroxide spin label, 2,2,6,6-tetramethyl-piperidine-N-oxyl-4-trimethylammonium (Cat1), was encapsulated in two types of liposomes, phosphatidylserine/phosphatidylcholine (PS/PC) and phosphatidylserine/distearoylphosphatidylcholine/dipalmitoylphosphatidyl choline (PS/DSPC/DPPC). Hydroxylamine 23-32 GIT ArfGAP 1 Mus musculus 104-108 35465922-4 2022 Rotational mobility of the nitroxide spin probe, TEMPOL, resolves and tracks two solvent components, the protein-associated domain (PAD; akin to hydration layer) and surrounding mesodomain, through their distinct temperature- and confinement-dependent values of tau and normalized weight. Hydroxylamine 27-36 chromosome 1 open reading frame 210 Homo sapiens 49-55 35465922-4 2022 Rotational mobility of the nitroxide spin probe, TEMPOL, resolves and tracks two solvent components, the protein-associated domain (PAD; akin to hydration layer) and surrounding mesodomain, through their distinct temperature- and confinement-dependent values of tau and normalized weight. Hydroxylamine 27-36 microtubule associated protein tau Homo sapiens 262-265 2592363-8 1989 Both fatty acids are attached post-translationally to p64 through a hydroxylamine-sensitive linkage, suggesting that acylation of this protein is catalyzed by a palmitoyl transferase with relaxed specificity for fatty acid substrates. Hydroxylamine 68-81 interleukin 2 receptor, gamma chain Mus musculus 54-57 2482076-9 1989 Both the fragments and the MBP decreased the lipid chain mobility as recorded by the C-5 atom and C-12 atom position nitroxide-PG spin-labels, in a manner which followed the protein binding curves. Hydroxylamine 117-126 myelin basic protein Bos taurus 27-30 2554973-4 1989 Using this nitroxide-liposome system, the respiration of liver slices was measured successfully, whereas such measurements using free cat1 were complicated by rapid reduction of the nitroxide. Hydroxylamine 182-191 GIT ArfGAP 1 Homo sapiens 134-138 2681210-7 1989 Furthermore, this band is sensitive to hydroxylamine treatment and shows specific incorporation of [3H]palmitate, strongly suggesting that it is the palmitoylated form of p21, which is the biologically active form of the protein. Hydroxylamine 39-52 H3 histone pseudogene 16 Homo sapiens 171-174 2551971-6 1989 Mammalian thioredoxin, a competitive inhibitor of nitroxide reduction by thioredoxin reductase, significantly stimulates nitroxide scavenging in skin homogenate. Hydroxylamine 50-59 thioredoxin Homo sapiens 10-21 2551971-6 1989 Mammalian thioredoxin, a competitive inhibitor of nitroxide reduction by thioredoxin reductase, significantly stimulates nitroxide scavenging in skin homogenate. Hydroxylamine 50-59 thioredoxin Homo sapiens 73-84 2551971-6 1989 Mammalian thioredoxin, a competitive inhibitor of nitroxide reduction by thioredoxin reductase, significantly stimulates nitroxide scavenging in skin homogenate. Hydroxylamine 121-130 thioredoxin Homo sapiens 10-21 2551971-6 1989 Mammalian thioredoxin, a competitive inhibitor of nitroxide reduction by thioredoxin reductase, significantly stimulates nitroxide scavenging in skin homogenate. Hydroxylamine 121-130 thioredoxin Homo sapiens 73-84 2551971-10 1989 At least for mouse epidermis, reduction of a variety of nitroxides is a complex phenomenon involving enzymatic and nonenzymatic mechanisms and cannot be used as a specific assay for an enzyme, e.g., thioredoxin reductase. Hydroxylamine 56-66 thioredoxin Homo sapiens 199-210 2605226-7 1989 Although chicken green was contaminated with a small amount of rhodopsin having a similar spectral shape, the maximum of its difference spectrum was located at 508 nm by taking advantage of the difference in susceptibility against hydroxylamine between these pigments. Hydroxylamine 231-244 rhodopsin Gallus gallus 63-72 2482181-14 1989 Epitope analysis using actin fragments generated by limited proteolysis and selective cleavage using hydroxylamine indicate that this antibody is directed against a rather limited region within the N-terminus of actin. Hydroxylamine 101-114 actin epsilon 1 Bos taurus 23-28 2482181-14 1989 Epitope analysis using actin fragments generated by limited proteolysis and selective cleavage using hydroxylamine indicate that this antibody is directed against a rather limited region within the N-terminus of actin. Hydroxylamine 101-114 actin epsilon 1 Bos taurus 212-217 2777764-3 1989 Treatment of these heteroduplexes with hydroxylamine followed by cleavage of the cDNA strand at reactive bases by piperidine identified mismatches in the pro-alpha 1(I) cDNA in four patients. Hydroxylamine 39-52 PROA Homo sapiens 154-163 2551301-0 1989 Detection of free radicals as intermediates in the methemoglobin formation from oxyhemoglobin induced by hydroxylamine. Hydroxylamine 105-118 hemoglobin subunit gamma 2 Homo sapiens 51-64 2551301-5 1989 The third one is a low-spin iron-(III)-complex, possibly the methemoglobin-hydroxylamine adduct. Hydroxylamine 75-88 hemoglobin subunit gamma 2 Homo sapiens 61-74 2551301-9 1989 The identification of the radical intermediates detected in the hydroxylamine-induced methemoglobin formation contributes to a more detailed understanding of the reaction sequence. Hydroxylamine 64-77 hemoglobin subunit gamma 2 Homo sapiens 86-99 2505770-6 1989 Catalase, an enzyme known to oxidize hydroxylamine to nitric oxide, was present in homogenates of intact and endothelium-denuded rings. Hydroxylamine 37-50 catalase Rattus norvegicus 0-8 2476174-8 1989 Comparison of ESR results obtained from spin-labeling methylamine-treated or protease-reacted alpha 2M with those from spin-labeling of the free SH groups in intermediate-form alpha 2M shows that trapped protease influences the mobility of the attached nitroxide either through direct contact or by producing a different conformation from that present in methylamine-treated or intermediate-form alpha 2M. Hydroxylamine 253-262 alpha-2-macroglobulin Homo sapiens 94-102 2476174-8 1989 Comparison of ESR results obtained from spin-labeling methylamine-treated or protease-reacted alpha 2M with those from spin-labeling of the free SH groups in intermediate-form alpha 2M shows that trapped protease influences the mobility of the attached nitroxide either through direct contact or by producing a different conformation from that present in methylamine-treated or intermediate-form alpha 2M. Hydroxylamine 253-262 alpha-2-macroglobulin Homo sapiens 176-184 2476174-8 1989 Comparison of ESR results obtained from spin-labeling methylamine-treated or protease-reacted alpha 2M with those from spin-labeling of the free SH groups in intermediate-form alpha 2M shows that trapped protease influences the mobility of the attached nitroxide either through direct contact or by producing a different conformation from that present in methylamine-treated or intermediate-form alpha 2M. Hydroxylamine 253-262 alpha-2-macroglobulin Homo sapiens 176-184 2543460-3 1989 Thus long chain spin labels with the nitroxide group near the terminal methyl of the chain, such as 16-doxylstearic acid, its methyl ester, or a phosphatidylglycerol spin label containing 16-doxylstearic acid (PG-SL), are more motionally restricted and/or ordered in the interdigitated bilayer than in the non-interdigitated bilayer. Hydroxylamine 37-46 spindlin 1 Homo sapiens 16-20 2540962-8 1989 Deacylation of human erythrocyte AChE by an alkaline treatment with hydroxylamine rendered the amphiphilic AChE susceptible to PtdIns-specific PLC with the consequent release of hydrophilic AChE. Hydroxylamine 68-81 acetylcholinesterase (Cartwright blood group) Homo sapiens 33-37 2540962-8 1989 Deacylation of human erythrocyte AChE by an alkaline treatment with hydroxylamine rendered the amphiphilic AChE susceptible to PtdIns-specific PLC with the consequent release of hydrophilic AChE. Hydroxylamine 68-81 acetylcholinesterase (Cartwright blood group) Homo sapiens 107-111 2540962-8 1989 Deacylation of human erythrocyte AChE by an alkaline treatment with hydroxylamine rendered the amphiphilic AChE susceptible to PtdIns-specific PLC with the consequent release of hydrophilic AChE. Hydroxylamine 68-81 heparan sulfate proteoglycan 2 Homo sapiens 143-146 2540962-8 1989 Deacylation of human erythrocyte AChE by an alkaline treatment with hydroxylamine rendered the amphiphilic AChE susceptible to PtdIns-specific PLC with the consequent release of hydrophilic AChE. Hydroxylamine 68-81 acetylcholinesterase (Cartwright blood group) Homo sapiens 107-111 2784671-6 1989 When [14C]C3-pFc" covalent complexes were treated with 1 M-NH2OH and loaded onto a Bio-Gel P-4 column, a radioactive peak of 3 kDa was obtained. Hydroxylamine 59-64 exosome component 10 Homo sapiens 91-94 2542235-0 1989 Localization of the active center of nitroxide radical reduction in rat liver microsomes: its relation to cytochrome P-450 and membrane fluidity. Hydroxylamine 37-46 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 106-122 2542235-4 1989 Thus, the nitroxide radicals of the SLSs and TEMPOL seem to be reduced by the combined action of NADPH-cytochrome P-450 reductase and cytochrome P-450. Hydroxylamine 10-19 cytochrome p450 oxidoreductase Rattus norvegicus 97-129 2542235-4 1989 Thus, the nitroxide radicals of the SLSs and TEMPOL seem to be reduced by the combined action of NADPH-cytochrome P-450 reductase and cytochrome P-450. Hydroxylamine 10-19 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 103-119 3066688-3 1988 In order to examine the function of this protein, the TEF2 gene, which encodes EF-1 alpha in Saccharomyces cerevisiae, was mutagenized in vitro with hydroxylamine. Hydroxylamine 149-162 translation elongation factor EF-1 alpha Saccharomyces cerevisiae S288C 54-58 2509200-9 1989 Upon treatment of the R*ret-Te complex by a high concentration of hydroxylamine, the retinal can be removed from the rhodopsin. Hydroxylamine 66-79 rhodopsin Homo sapiens 117-126 2478197-5 1989 This antigenic site was located on the N-terminal sequence of OSCP, between residues 1 and 72, as demonstrated after chemical cleavage of OSCP with formic acid, hydroxylamine and partial cleavage with cyanogen bromide. Hydroxylamine 161-174 ATP synthase peripheral stalk subunit OSCP Homo sapiens 62-66 2540499-1 1989 Decay of metarhodopsin II was accelerated by hydroxylamine treatment or dark incubation of metarhodopsin II at 30 degrees C. The products thus obtained after decay of metarhodopsin II induced GTPase activity on transducin as well as metarhodopsin II suggesting that rhodopsin could activate transducin after the decay of metarhodopsin II intermediate. Hydroxylamine 45-58 rhodopsin Bos taurus 13-22 2914607-4 1989 Experiments using hydroxylamine as an artificial quencher of rhodopsin activity suggest that calcium acts upon rhodopsin kinase and not upon the rate of the GTPase. Hydroxylamine 18-31 rhodopsin Homo sapiens 61-70 2914607-4 1989 Experiments using hydroxylamine as an artificial quencher of rhodopsin activity suggest that calcium acts upon rhodopsin kinase and not upon the rate of the GTPase. Hydroxylamine 18-31 rhodopsin Homo sapiens 111-120 2845967-0 1988 Electron spin resonance study of light-induced conformational changes in nitroxide labelled bovine rhodopsin. Hydroxylamine 73-82 rhodopsin Bos taurus 99-108 2847589-0 1988 Characterization of protein spin labeling by maleimide: evidence for nitroxide reduction. Hydroxylamine 69-78 spindlin 1 Homo sapiens 28-32 3219359-8 1988 Incubation of N alpha-[(acetylthio)acetyl]-D-Phe-Pro-Arg-thrombin with 5-(iodoacetamido)fluorescein in the presence of NH2OH resulted in incorporation of 0.96 mol of the fluorescence probe/mol of active sites and the appearance of fluorescein fluorescence associated with the active site containing B-chain on sodium dodecyl sulfate-polyacrylamide gels. Hydroxylamine 119-124 coagulation factor II, thrombin Homo sapiens 57-65 3219359-9 1988 Fluorescence labeling of thrombin required reaction of the inhibitor at the active site as well as subsequent generation of the thiol group with NH2OH. Hydroxylamine 145-150 coagulation factor II, thrombin Homo sapiens 25-33 3041914-2 1988 Both fatty acids, when incorporated into the transferrin receptor, can be released by treating the protein with 1 M hydroxylamine at pH 7.0. Hydroxylamine 116-129 transferrin receptor protein 1 Ovis aries 45-65 2839365-4 1988 When rhodopsin deactivation is accelerated (in the presence of NH2OH), PA-signal recovery is also accelerated. Hydroxylamine 63-68 rhodopsin Bos taurus 5-14 3166978-7 1988 Deacylation of TF with hydroxylamine resulted in the spontaneous generation of disulfide-linked TF dimers. Hydroxylamine 23-36 coagulation factor III, tissue factor Homo sapiens 15-17 3166978-7 1988 Deacylation of TF with hydroxylamine resulted in the spontaneous generation of disulfide-linked TF dimers. Hydroxylamine 23-36 coagulation factor III, tissue factor Homo sapiens 96-98 2835106-3 1988 This inactivation of cytochrome b5 is reversed with hydroxylamine, which also suggests but does not prove histidine involvement. Hydroxylamine 52-65 cytochrome b5 type A Homo sapiens 21-34 2835102-0 1988 Localizing the nitroxide group of fatty acid and voltage-sensitive spin-labels in phospholipid bilayers. Hydroxylamine 15-24 spindlin 1 Homo sapiens 67-71 2839161-0 1988 Estimation of the distance between the divalent cation binding site of des-1-41-light chain-activated bovine plasma protein C and a nitroxide spin label attached to the active-site serine residue. Hydroxylamine 132-141 delta 4-desaturase, sphingolipid 1 Bos taurus 71-76 3054507-2 1988 A plasmid carrying RPA190, the gene encoding the largest subunit of the enzyme, was subjected to in vitro mutagenesis with hydroxylamine. Hydroxylamine 123-136 DNA-directed RNA polymerase I core subunit RPA190 Saccharomyces cerevisiae S288C 19-25 3128179-7 1988 The data are consistent with a mechanism where the substrate undergoes four-electron reduction to form a hydroxylamine, which is susceptible to nucleophilic attack at C-4. Hydroxylamine 105-118 complement C4A (Rodgers blood group) Homo sapiens 167-170 2839161-1 1988 The paramagnetic effect of Mn2+ on the electron paramagnetic resonance spectrum of a nitroxide spin label covalently attached to the active-site serine residue of des-1-41-light chain bovine plasma-activated protein C, and situated at a distance of approximately 1.2 nm from this amino acid, has been utilized to estimate the distance on the enzyme surface between the single Mn2+ site and the free electron of the spin label. Hydroxylamine 85-94 delta 4-desaturase, sphingolipid 1 Bos taurus 163-168 3127211-10 1988 This fatty-acid--PLAP bond was sensitive to pH 10 hydroxylamine treatment indicating an O-ester linkage. Hydroxylamine 50-63 alkaline phosphatase, placental Homo sapiens 17-21 2838099-1 1988 Short-pulse saturation-recovery (SR) electron spin resonance (ESR) methods have been used to measure the lateral diffusion of a nitroxide-labeled cholesterol analogue (3-spiro-[2"-(N-oxyl-4",4"-dimethyloxazoladine)]-cholestane, CSL) in multilamellar liposomal dispersions. Hydroxylamine 128-137 chorionic somatomammotropin hormone like 1 Homo sapiens 228-231 3129552-4 1988 It appears as if this is due to oxidation of dapsone by myeloperoxidase to the hydroxylamine, followed by nonenzymatic oxidation of the hydroxylamine to the nitroderivative. Hydroxylamine 79-92 myeloperoxidase Homo sapiens 56-71 2833253-9 1988 Inhibitors of superoxide dismutase (sodium diethyldithiocarbamate) and catalase (hydroxyl amine and 3, amino 1,2,4-triazole) augmented Pf-II-mediated cutaneous photosensitization. Hydroxylamine 81-95 catalase Mus musculus 14-79 3269256-4 1988 For example, 1-nitropyrene (1-NP) is activated by enzymatic reduction to 1-nitrosopyrene (1-NOP), followed by reduction to the hydroxylamine, which undergoes decomposition to yield a nitrenium ion, that reacts with DNA. Hydroxylamine 127-140 prepronociceptin Homo sapiens 92-95 2966154-10 1988 Moreover, removal of the acetoacetyl group from the lysine residues of apoB by hydroxylamine reestablished the interaction of LDL2 with Lp(a). Hydroxylamine 79-92 apolipoprotein B Homo sapiens 71-75 2966154-10 1988 Moreover, removal of the acetoacetyl group from the lysine residues of apoB by hydroxylamine reestablished the interaction of LDL2 with Lp(a). Hydroxylamine 79-92 lipoprotein(a) Homo sapiens 136-141 3126736-4 1988 Other pyrazoline derivatives such as BW 755C, but also, in a more general manner, different compounds containing phenol, aniline, hydrazine, hydroxylamine or hydrazide functions act as reducing substrates for decomposition of 13-HPOD by L1. Hydroxylamine 141-154 seed linoleate 13S-lipoxygenase-1 Glycine max 237-239 2439371-4 1987 Covalent binding is abolished when the reaction of alpha 2M with the protease is carried out in the presence of hydroxylamine. Hydroxylamine 112-125 alpha-2-macroglobulin Homo sapiens 51-59 2446598-6 1987 Palmitic acid is covalently bound to PLP and DM-20, because 70 and 92% of the radioactivity was removed from proteolipid proteins after treatment with hydroxylamine and methanolic NaOH respectively. Hydroxylamine 151-164 proteolipid protein 1 Rattus norvegicus 37-40 3112579-7 1987 Nineteen independently isolated adr1 mutations induced by hydroxylamine were found at nine different amino-acid positions, seven of which are in the two finger domains. Hydroxylamine 58-71 DNA-binding transcription factor ADR1 Saccharomyces cerevisiae S288C 32-36 3038021-4 1987 One of the reduction systems, consisting of the photosensitizer FMN and the photoreductant EDTA, was used to study both anaerobic reduction and O2-dependent reoxidation of some of the nitroxides. Hydroxylamine 184-194 formin 1 Homo sapiens 64-67 3367699-2 1988 Hydroxylamine stimulated the inhibition of acid CEH activity by Cu2+ but not that by Zn2+, Fe2+, Co2+, Mn2+, Ca2+, Mg2+ and Hg2+. Hydroxylamine 0-13 epoxide hydrolase 2 Rattus norvegicus 48-51 3367699-5 1988 On the other hand, ascorbic acid was found to replace the stimulation by hydroxylamine of the Cu2+-dependent inhibition of acid CEH activity but the effects of ascorbic acid progressively became smaller with prolongation of the preincubation time. Hydroxylamine 73-86 epoxide hydrolase 2 Rattus norvegicus 128-131 2831973-0 1988 Evidence for multiple conformational changes in the active center of thrombin induced by complex formation with thrombomodulin: an analysis employing nitroxide spin-labels. Hydroxylamine 150-159 coagulation factor II, thrombin Homo sapiens 69-77 3315005-12 1987 Parasitized mice treated with chloroquine six hours prior to ESR measurements show less nitroxide reducing capacity than do untreated mice. Hydroxylamine 88-97 esterase 5 regulator Mus musculus 61-64 2827156-2 1987 To identify regions of the hTNF protein involved in binding hTNF to its receptor, we prepared five synthetic peptides [hTNF-(1-15), hTNF-(1-31), hTNF-(65-79), hTNF-(98-111), and hTNF-(124-141)] and two hydroxylamine cleavage fragments [hTNF-(1-39) and hTNF-(40-157)] of hTNF. Hydroxylamine 202-215 tumor necrosis factor Homo sapiens 27-31 3676250-2 1987 After IgG affinity purification of the fusion proteins from the growth medium of Staphylococcus aureus or Escherichia coli, native IGF-I was released by cleavage of an Asn-Gly peptide bond with hydroxylamine. Hydroxylamine 194-207 insulin like growth factor 1 Homo sapiens 131-136 3036257-1 1987 The ESR signal of nitrosyl complexes appears in mouse liver after hydroxylamine injection in vivo. Hydroxylamine 66-79 esterase 5 regulator Mus musculus 4-7 3105619-11 1987 Conversion of tPA complex to enzymatically active free tPA also occurs with brief SDS exposure followed by incubation in the presence of excess Triton X-100 or by hydroxylamine treatment. Hydroxylamine 163-176 chromosome 20 open reading frame 181 Homo sapiens 14-17 3028490-6 1987 Lipoamide dehydrogenase and glutathione reductase are also capable of reducing nitroxide spin labels, a finding considered of relevance to the reported reduction of such spin labels by neuroblastoma cells. Hydroxylamine 79-88 dihydrolipoamide dehydrogenase Homo sapiens 0-23 3105619-11 1987 Conversion of tPA complex to enzymatically active free tPA also occurs with brief SDS exposure followed by incubation in the presence of excess Triton X-100 or by hydroxylamine treatment. Hydroxylamine 163-176 chromosome 20 open reading frame 181 Homo sapiens 55-58 3573147-5 1987 While none of these proteins was labeled with 3H-palmitate, the preS1 protein but not the preS2 or S protein incorporated 3H-myristate via a hydroxylamine-resistant amide linkage. Hydroxylamine 141-154 large envelope protein;middle envelope protein;small envelope protein Hepatitis B virus 64-69 3030756-6 1987 This was also suggested by hydroxylamine cleavage of the two complexes as the cleavage gave the fluorescent 41-kDa fragment which could not be produced unless plasma gelsolin was cross-linked at the NH2-terminal segment comprising 12 amino acids. Hydroxylamine 27-40 gelsolin Homo sapiens 166-174 3028490-6 1987 Lipoamide dehydrogenase and glutathione reductase are also capable of reducing nitroxide spin labels, a finding considered of relevance to the reported reduction of such spin labels by neuroblastoma cells. Hydroxylamine 79-88 glutathione-disulfide reductase Homo sapiens 28-49 2879570-3 1987 Lauric acid was detected following hydroxylamine treatment of the enzyme, suggesting the occurrence of a fatty acid-type, covalent, posttranslational modification of transglutaminase. Hydroxylamine 35-48 protein-glutamine gamma-glutamyltransferase 2 Cavia porcellus 166-182 3439474-5 1987 The results obtained show that the hydroxylamine derivatives of barbituric acid HB-2 (2-hydroxylamino-5-ethyl-5-propylbarbituric acid) and HB-7 (2-hydroxylamino-5-ethyl-5 sec. Hydroxylamine 35-48 activated leukocyte cell adhesion molecule Rattus norvegicus 80-84 3099764-7 1986 Studies of the reactivity of the coenzyme (pyridoxal 5"-phosphate) of glycogen phosphorylase b with hydroxylamine show that this reaction only occurs when the pH value of solution is below 6.5 and the hydrolysis of imine bond has started. Hydroxylamine 100-113 glycogen phosphorylase B Homo sapiens 70-94 2432837-11 1986 Additionally, hydroxylamine cleavage of an Asn-Gly bond in prefetuin localized one of the N-linked carbohydrate side chains to the middle of the polypeptide chain of native fetuin. Hydroxylamine 14-27 alpha-2-HS-glycoprotein Oryctolagus cuniculus 62-68 2948553-10 1986 Changes in buffer composition, including high concentrations of glycine for lambda repressor and of imidazole or hydroxylamine for LexA, indicated that solvent components other than water do not participate in the rate-determining step. Hydroxylamine 113-126 DNA repair system Escherichia coli 131-135 3745178-7 1986 The effects of the nucleophiles hydroxylamine, hydrazine, and ethylenediamine on Km and Vmax for lipoprotein lipase catalyzed hydrolysis of p-nitrophenyl laurate are consistent with trapping of a lauryl-lipoprotein lipase intermediate. Hydroxylamine 32-45 lipoprotein lipase Bos taurus 97-115 3601356-1 1987 The levels of rhodopsin in isolated retinae of two mouse genotypes have been estimated by measuring with a scanning spectrophotometer the apparent absorbance change at 500 nm after photo-bleaching the retinae for 2 min in the presence of hydroxylamine. Hydroxylamine 238-251 rhodopsin Mus musculus 14-23 3026342-14 1986 Reaction of diethyl pyrocarbonate-treated alpha 2M with hydroxylamine reversed derivatization of 43 of the 53 histidine residues. Hydroxylamine 56-69 alpha-2-macroglobulin Homo sapiens 42-50 3752953-5 1986 3-ABP was also converted to the corresponding hydroxylamine, nitroso and nitro compound although the latter may have arisen via a non enzymic process. Hydroxylamine 46-59 amine oxidase, copper containing 1 Rattus norvegicus 2-5 3700425-8 1986 Incubation of native LCAT with arachidonyl-CoA or the lecithin-apo-A-I proteoliposome resulted in acylation of three enzyme sites, only one of which was stable to neutral hydroxylamine after denaturation. Hydroxylamine 171-184 lecithin-cholesterol acyltransferase Homo sapiens 21-25 3005308-7 1986 Hydroxylamine treatment resulted in the deacylation of apo-A-I. Hydroxylamine 0-13 apolipoprotein A1 Homo sapiens 55-62 2424497-1 1986 Three nitroxide spin-labeled monoderivatives of bovine pancreatic trypsin inhibitor were prepared with the amino-specific reagent succinimidyl 1-oxy-2,2,5,5-tetramethyl-3-pyrroline-3-carboxylate. Hydroxylamine 6-15 trophoblast Kunitz domain protein 1 Bos taurus 66-83 2422647-1 1986 The complete amino acid sequence of the prostate-specific antigen (PA) from human seminal plasma has been determined from analyses of the peptides generated by cyanogen bromide, hydroxylamine, endoproteinases Arg-C and Lys-C. Hydroxylamine 178-191 kallikrein related peptidase 3 Homo sapiens 40-70 3634756-4 1986 Elastase II was fragmented into several peptides by chemical cleavages with CNBr at the two methionine residues, 99 and 180 (chymotrypsinogen numbering), and with hydroxylamine at the peptide bond following DIP-Ser195. Hydroxylamine 163-176 chymotrypsin-like elastase family member 2A Bos taurus 0-11 3006745-1 1985 Antibodies have been elicited to the nitroxide spin-label 4-maleimido-2,2,6,6-tetramethyl-piperidinyl-1-oxy conjugated, via protein sulfhydryl groups, to bovine serum albumin. Hydroxylamine 37-46 albumin Homo sapiens 161-174 3841480-1 1985 Hydroxylamine-containing analogues of putrescine and cadaverine have been found effective in inhibiting the mouse liver ornithine decarboxylase, the best among synthesized were 1-aminooxy-3-aminopropane (I50 2.10(-8) M) and 1-aminooxy-4-aminobutane (I50 2.10(-7) M). Hydroxylamine 0-13 ornithine decarboxylase, structural 1 Mus musculus 120-143 2996648-6 1985 While CSL and 5-PC both reflect the high ordering of the bilayer close to the lipid-water interface, CSL appears to be located close enough to the water for the nitroxide to be involved in hydrogen bonding with water molecules. Hydroxylamine 161-170 chorionic somatomammotropin hormone like 1 Homo sapiens 101-104 2867184-6 1985 Staining was prevented by the SSAO inhibitors hydroxylamine (1 microM) and semicarbazide (1 mM), but not by the MAO inhibitor, clorgyline (1 mM). Hydroxylamine 46-59 amine oxidase, copper containing 3 Rattus norvegicus 30-34 4034725-1 1985 We have isolated and characterized cop, copts, and repam mutants of plasmid mini-F after in vitro mutagenesis with hydroxylamine. Hydroxylamine 115-128 caspase recruitment domain family member 16 Homo sapiens 35-38 3927920-0 1985 Investigation of rhodopsin catalyzed G-protein GTP-binding using [35S] GTP gamma S--effects of regeneration and hydroxylamine. Hydroxylamine 112-125 rhodopsin Homo sapiens 17-26 2985057-0 1985 Spin-labeled erythrocyte membranes: direct identification of nitroxide-conjugated proteins. Hydroxylamine 61-70 spindlin 1 Homo sapiens 0-4 2985057-1 1985 The covalent incorporation of a spin-labeled analog of N-ethyl maleimide into erythrocyte membrane proteins has been monitored by electron paramagnetic resonance spectroscopy and the individually labeled proteins detected by immunoblotting techniques, using an anti-nitroxide antibody, following electrophoretic separation of the membrane components. Hydroxylamine 266-275 spindlin 1 Homo sapiens 32-36 3839996-2 1985 Hydroxylamine was oxidized to NO2- much more rapidly than was NH3, and in this case superoxide dismutase or the chelating agents inhibited but catalase or the HO. Hydroxylamine 0-13 catalase Homo sapiens 143-151 3160706-4 1985 Hydroxylamine treatment of the labeled ATPase has shown that the phosphorylation was additive to be acylphosphate formed on the ATPase during the reaction cycle. Hydroxylamine 0-13 dynein axonemal heavy chain 8 Homo sapiens 39-45 3160706-4 1985 Hydroxylamine treatment of the labeled ATPase has shown that the phosphorylation was additive to be acylphosphate formed on the ATPase during the reaction cycle. Hydroxylamine 0-13 dynein axonemal heavy chain 8 Homo sapiens 128-134 4066150-4 1985 The HS-CH2CH2CO group was introduced onto the synthetic neurokinin A by reaction of 3-(S-acetyl-thiopropionyl)-thiazolidine-2-thione, followed by deacetylation with hydroxylamine. Hydroxylamine 165-178 tachykinin precursor 1 Homo sapiens 56-68 2996591-9 1985 The formed hydroxylamine then uncouples the cytochrome P-450 system to generate superoxide and hydrogen peroxide. Hydroxylamine 11-24 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 44-60 2863750-10 1985 Our results indicate that: allosteric interactions of metal ions are important to the regulation of the enzyme, the free radical nitroxide as well as hydrogen peroxide enhances enzyme activity, agonist occupancy of the muscarinic cholinergic receptor activates neutrophil guanylate cyclase probably through a mechanism involving calcium influx and the activation of the lipoxygenase pathway, and a TFP-sensitive site (possibly calmodulin) is involved in the selective regulation of basal enzyme activity. Hydroxylamine 129-138 calmodulin 1 Homo sapiens 427-437 4020299-8 1985 Hydroxylamine inhibited phosphatidylserine decarboxylase, but did not prevent the reactions stimulated by Triton X-100. Hydroxylamine 0-13 ATN24_RS01580 Clostridium butyricum 24-56 3886645-5 1985 Addition of hydroxylamine to ethoxyformylated prothrombin reversed the loss of fibrinogen-clotting activity. Hydroxylamine 12-25 coagulation factor II, thrombin Homo sapiens 46-57 3886645-5 1985 Addition of hydroxylamine to ethoxyformylated prothrombin reversed the loss of fibrinogen-clotting activity. Hydroxylamine 12-25 fibrinogen beta chain Homo sapiens 79-89 3968643-4 1985 However, the hydroxylamine is readily converted nonenzymatically to the nitroso derivative, and reducing agents such as ascorbate and NADPH, which reduce the nitroso derivative to the hydroxylamine, blocked covalent binding. Hydroxylamine 13-26 2,4-dienoyl-CoA reductase 1 Homo sapiens 134-139 6239898-1 1984 We have prepared C3b covalently linked to IgG via a hydroxylamine-sensitive bond between the C3b alpha" chain and sites predominantly, but not exclusively, located in the IgG heavy chain. Hydroxylamine 52-65 endogenous retrovirus group K member 3 Homo sapiens 17-20 3968643-4 1985 However, the hydroxylamine is readily converted nonenzymatically to the nitroso derivative, and reducing agents such as ascorbate and NADPH, which reduce the nitroso derivative to the hydroxylamine, blocked covalent binding. Hydroxylamine 184-197 2,4-dienoyl-CoA reductase 1 Homo sapiens 134-139 6239898-1 1984 We have prepared C3b covalently linked to IgG via a hydroxylamine-sensitive bond between the C3b alpha" chain and sites predominantly, but not exclusively, located in the IgG heavy chain. Hydroxylamine 52-65 endogenous retrovirus group K member 3 Homo sapiens 93-96 6509025-1 1984 The nuclear magnetic resonance spectra of an Fab fragment of a monoclonal antibody specifically directed against a nitroxide spin-label hapten have been recorded at different concentrations of the hapten. Hydroxylamine 115-124 FA complementation group B Homo sapiens 45-48 6091764-7 1984 By contrast, the spin label on the membrane-bound enzyme, D-beta-hydroxybutyrate dehydrogenase (D-beta-hydroxybutyrate:NAD+ oxidoreductase, EC 1.1.1.30), is completely immobilized and exhibits two distinct spectral components for spin-labeled NAD+, which appear to differ in the polarity of the environment of the nitroxide. Hydroxylamine 314-323 hydroxysteroid 17-beta dehydrogenase 6 Homo sapiens 124-138 6097141-0 1984 Reevaluation of the reactivity of hydroxylamine with O2-/HO2. Hydroxylamine 34-47 heme oxygenase 2 Homo sapiens 57-60 6097141-1 1984 The reactivity of hydroxylamine with HO2/O2- radicals was studied by pulse radiolysis and stopped-flow photolysis over a pH range of 1.1-10.5. Hydroxylamine 18-31 heme oxygenase 2 Homo sapiens 37-40 6435697-1 1984 A method to break the pyridoxal 5"-phosphate (PLP)-phosphorylase b bond using hydroxylamine and slightly acid pH is put forward and described in the present paper. Hydroxylamine 78-91 pyridoxal phosphatase Homo sapiens 22-44 24277329-2 1984 Preliminary information on the metabolic fate, pharmacokinetic behavior, stability in tissues, and chemical reduction of two prototypic nitroxides, PCA and TES, is presented. Hydroxylamine 136-146 testin LIM domain protein Canis lupus familiaris 156-159 6435697-1 1984 A method to break the pyridoxal 5"-phosphate (PLP)-phosphorylase b bond using hydroxylamine and slightly acid pH is put forward and described in the present paper. Hydroxylamine 78-91 pyridoxal phosphatase Homo sapiens 46-49 16663546-7 1984 However, ferric leghemoglobin was formed by treating the nodule slices with hydroxylamine, and this was confirmed by complexing the ferric leghemoglobin to acetate, fluoride, or nicotinic acid. Hydroxylamine 76-89 leghemoglobin A Glycine max 16-29 6468381-14 1984 This effect was shown to be due to conversion of partially digested rhodopsin to a photolytic product that at room temperature lived for minutes even in the presence of NH2OH. Hydroxylamine 169-174 rhodopsin Bos taurus 68-77 6327697-2 1984 Hydroxylamine oxidoreductase from Nitrosomonas europeae catalyzes the oxidative conversion of NH2OH to NO-2. Hydroxylamine 94-99 hydroxysteroid 17-beta dehydrogenase 6 Homo sapiens 14-28 6617659-4 1983 A positive correlation was obtained between calmodulin-dependent increase in the rate of calcium transport and hydroxylamine-insensitive phosphoester formed by the calcium/calmodulin-regulated, membrane-bound protein kinase. Hydroxylamine 111-124 calmodulin-2 Canis lupus familiaris 44-54 6233802-1 1984 The calcium-dependent acylphosphate formed by the calcium transport ATPase of cardiac sarcoplasmic reticulum and the calcium-, calmodulin-dependent phosphoester(s) of sarcoplasmic reticulum fractions formed by a calcium-, calmodulin-dependent membrane-bound protein kinase can be distinguished by removal of calcium and/or magnesium by EDTA or hydroxylamine treatment of the acid denaturated membranes. Hydroxylamine 344-357 calmodulin 1 Homo sapiens 127-137 6095355-3 1984 This hydroxylamine is then further oxidized to the nitroxyl free radical norcocaine nitroxide by rat brain cytochrome P-450. Hydroxylamine 5-18 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 107-123 6642644-5 1983 Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of dithiothreitol and NH2OH eluates from the avidin-Sepharose showed that C3b bound to both heavy and light chains of the Ab. Hydroxylamine 80-85 complement C3 Homo sapiens 132-135 6698284-7 1984 Human GMP synthetase is inhibited by ATP, dATP, azaserine, and hydroxylamine. Hydroxylamine 63-76 guanine monophosphate synthase Homo sapiens 6-20 6698715-1 1984 tert.-Butyl amino carbonate (1, tert.-butyloxycarbonyloxyamine) was prepared by reaction of hydroxylamine with di-tert.-butyl dicarbonate (2). Hydroxylamine 92-105 telomerase reverse transcriptase Homo sapiens 0-4 6698715-1 1984 tert.-Butyl amino carbonate (1, tert.-butyloxycarbonyloxyamine) was prepared by reaction of hydroxylamine with di-tert.-butyl dicarbonate (2). Hydroxylamine 92-105 telomerase reverse transcriptase Homo sapiens 32-36 6698715-1 1984 tert.-Butyl amino carbonate (1, tert.-butyloxycarbonyloxyamine) was prepared by reaction of hydroxylamine with di-tert.-butyl dicarbonate (2). Hydroxylamine 92-105 telomerase reverse transcriptase Homo sapiens 32-36 6687316-2 1983 Because this inhibition is reversed by hydroxylamine, we suggest that the chick oviduct progesterone receptor contains one or more histidine residues that regulate progestin binding. Hydroxylamine 39-52 progesterone receptor Gallus gallus 88-109 6197765-0 1983 The alpha 2 macroglobulin/thrombin interaction in the presence of hydroxylamine. Hydroxylamine 66-79 alpha-2-macroglobulin Homo sapiens 4-25 6197765-0 1983 The alpha 2 macroglobulin/thrombin interaction in the presence of hydroxylamine. Hydroxylamine 66-79 coagulation factor II, thrombin Homo sapiens 26-34 6617659-4 1983 A positive correlation was obtained between calmodulin-dependent increase in the rate of calcium transport and hydroxylamine-insensitive phosphoester formed by the calcium/calmodulin-regulated, membrane-bound protein kinase. Hydroxylamine 111-124 calmodulin-2 Canis lupus familiaris 172-182 6197765-1 1983 The influence of a primary amine, hydroxylamine, on the interaction between alpha 2 macroglobulin (alpha 2M) and thrombin was analyzed by electrophoretic and enzymatic methods. Hydroxylamine 34-47 alpha-2-macroglobulin Homo sapiens 76-97 6197765-1 1983 The influence of a primary amine, hydroxylamine, on the interaction between alpha 2 macroglobulin (alpha 2M) and thrombin was analyzed by electrophoretic and enzymatic methods. Hydroxylamine 34-47 alpha-2-macroglobulin Homo sapiens 99-107 6193011-2 1983 The thrombin-alpha 2M binding is normally covalent, but the presence of hydroxylamine during the reaction leads to the formation of a non-covalent complex. Hydroxylamine 72-85 coagulation factor II, thrombin Homo sapiens 4-12 6197765-1 1983 The influence of a primary amine, hydroxylamine, on the interaction between alpha 2 macroglobulin (alpha 2M) and thrombin was analyzed by electrophoretic and enzymatic methods. Hydroxylamine 34-47 coagulation factor II, thrombin Homo sapiens 113-121 6197765-2 1983 Hydroxylamine (final concentrations 0.01 M and 0.1 M) was added to the alpha 2M solution 3 to 5 min before thrombin. Hydroxylamine 0-13 alpha-2-macroglobulin Homo sapiens 71-79 6197765-2 1983 Hydroxylamine (final concentrations 0.01 M and 0.1 M) was added to the alpha 2M solution 3 to 5 min before thrombin. Hydroxylamine 0-13 coagulation factor II, thrombin Homo sapiens 107-115 6197765-5 1983 However the rate of inhibition of the clotting activity of thrombin was diminished in function of the hydroxylamine concentration. Hydroxylamine 102-115 coagulation factor II, thrombin Homo sapiens 59-67 6193011-2 1983 The thrombin-alpha 2M binding is normally covalent, but the presence of hydroxylamine during the reaction leads to the formation of a non-covalent complex. Hydroxylamine 72-85 alpha-2-macroglobulin Homo sapiens 13-21 7126647-1 1982 The isolation and characterization of a hybridoma cell line producing a monoclonal IgG1 antibody against a spin-label nitroxide group is described. Hydroxylamine 118-127 immunoglobulin heavy constant gamma 1 (G1m marker) Mus musculus 83-87 6305345-1 1983 When the myeloperoxidase-catalyzed peroxidation of acetoacetate proceeds in the presence of piperidinooxy free radical, methyl glyoxal is formed, and the nitroxide group is reduced to the secondary amine. Hydroxylamine 154-163 myeloperoxidase Homo sapiens 9-24 6184589-3 1982 Nitroprusside (NP), hydroxylamine (HA) and sodium azide (NaA) increased c-GMP levels and also enhanced amylase release in a dose-dependent manner; cyclic-AMP (c-AMP) levels were not affected. Hydroxylamine 20-33 5'-nucleotidase, cytosolic II Mus musculus 74-77 6184589-3 1982 Nitroprusside (NP), hydroxylamine (HA) and sodium azide (NaA) increased c-GMP levels and also enhanced amylase release in a dose-dependent manner; cyclic-AMP (c-AMP) levels were not affected. Hydroxylamine 35-37 5'-nucleotidase, cytosolic II Mus musculus 74-77 6885787-18 1983 When thrombin-PN complexes are dissociated with 1 M hydroxylamine a smaller form of PN (approximately 46 kilodaltons) is detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating that the complexed PN is proteolytically modified. Hydroxylamine 52-65 coagulation factor II, thrombin Homo sapiens 5-13 6312894-3 1983 For small molecules, such as cysteine, N-acetylcysteine, glutathione, and 2-mercaptoethanol, the spectrum is that of a freely rotating nitroxide while for the proteins, bovine serum albumin and myosin, the spectrum is characteristic of a strongly immobilized nitroxide spin label rigidly attached to the protein. Hydroxylamine 135-144 albumin Homo sapiens 176-189 6312894-3 1983 For small molecules, such as cysteine, N-acetylcysteine, glutathione, and 2-mercaptoethanol, the spectrum is that of a freely rotating nitroxide while for the proteins, bovine serum albumin and myosin, the spectrum is characteristic of a strongly immobilized nitroxide spin label rigidly attached to the protein. Hydroxylamine 135-144 myosin heavy chain 14 Homo sapiens 194-200 6303406-3 1983 Order parameter values and approximate rotational correlation times for the nitroxide spin labels indicated that ethanol consumption results in an adaptive decrease in bilayer membrane fluidity, while hibernation produces increases in fluidity. Hydroxylamine 76-85 spindlin 1 Rattus norvegicus 86-90 24264606-2 1983 The Tiron radical signal obtained from chloroplasts is sensitive to superoxide dismutase (EC 1.15.1.1) confirming that it is derived from[Formula: see text], oxygen-dependent and unaffected by 3-(3,4-dichlorophenyl)-1,1-dimethyl urea and hydroxylamine. Hydroxylamine 238-251 Susceptibility to lysis by alloreactive natural killer cells Homo sapiens 90-94 6838898-3 1983 The results indicate that phenylalanine exhibits weak hydrophobic binding to hemoglobin, and that the binding site is probably located within about 7-13 A of the nitroxide free electron of spin-labeled hemoglobin. Hydroxylamine 162-171 spindlin 1 Homo sapiens 189-193 6605834-1 1983 A modified hydroxylamine oxidation assay method coupled with a parallel line analysis to detect endogenous interfering substances, was used to determine superoxide dismutase (SOD). Hydroxylamine 11-24 Superoxide dismutase Oncorhynchus mykiss 153-173 7107590-6 1982 Treatment of myelin proteins or purified PLP with 1 M hydroxylamine released most of the radioactivity, indicating that [3H]palmitic acid was covalently bound by ester linkage to the proteins. Hydroxylamine 54-67 proteolipid protein 1 Rattus norvegicus 41-44 6291581-1 1982 Chicken pepsinogen has been spin-labeled by the attachment of four nitroxides to epsilon-amino groups near the protein"s amino terminus. Hydroxylamine 67-77 pepsinogen 3, group I (pepsinogen A) Gallus gallus 8-18 6980671-6 1982 The link between C4b and C4bp is partially destroyed by 1 M hydroxylamine at pH 9.0; its formation is strongly inhibited by 3.5 mM hydroxylamine or 60 mM methylamine at pH 9.0. Hydroxylamine 60-73 complement C4B (Chido blood group) Homo sapiens 17-20 6980671-6 1982 The link between C4b and C4bp is partially destroyed by 1 M hydroxylamine at pH 9.0; its formation is strongly inhibited by 3.5 mM hydroxylamine or 60 mM methylamine at pH 9.0. Hydroxylamine 60-73 complement component 4 binding protein alpha Homo sapiens 25-29 6980671-6 1982 The link between C4b and C4bp is partially destroyed by 1 M hydroxylamine at pH 9.0; its formation is strongly inhibited by 3.5 mM hydroxylamine or 60 mM methylamine at pH 9.0. Hydroxylamine 131-144 complement C4B (Chido blood group) Homo sapiens 17-20 6980671-6 1982 The link between C4b and C4bp is partially destroyed by 1 M hydroxylamine at pH 9.0; its formation is strongly inhibited by 3.5 mM hydroxylamine or 60 mM methylamine at pH 9.0. Hydroxylamine 131-144 complement component 4 binding protein alpha Homo sapiens 25-29 6810958-7 1982 The interaction of Cu(Lys)2 with the membrane was studied by the ESR method using stable nitroxide rabicais. Hydroxylamine 89-98 aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase Homo sapiens 19-27 6288003-5 1982 NAD+ activates NADH-dependent reduction of cytochrome c, and the apparent maximum velocity for this substrate increases more sharply with the concentration of NAD+ than for hydroxylamine. Hydroxylamine 173-186 cytochrome c, somatic Homo sapiens 43-55 6288003-6 1982 The simplest explanation is that NAD+ activation of hydroxylamine reduction derives solely from activation of steps involved in the reduction of cytochrome c, a flavin-mediated reaction, but these steps are only partly rate-limiting for the reduction of hydroxylamine. Hydroxylamine 52-65 cytochrome c, somatic Homo sapiens 145-157 6288003-7 1982 At 0.5 mM-NAD+, the apparent maximum velocity was 2.3 times higher for 0.1 mM-cytochrome c as substrate than for 100 mM-hydroxylamine, suggesting that the rate-limiting step during hydroxylamine reduction is a step that is not involved in cytochrome c reduction. Hydroxylamine 120-133 cytochrome c, somatic Homo sapiens 78-90 6288003-7 1982 At 0.5 mM-NAD+, the apparent maximum velocity was 2.3 times higher for 0.1 mM-cytochrome c as substrate than for 100 mM-hydroxylamine, suggesting that the rate-limiting step during hydroxylamine reduction is a step that is not involved in cytochrome c reduction. Hydroxylamine 120-133 cytochrome c, somatic Homo sapiens 239-251 6288003-7 1982 At 0.5 mM-NAD+, the apparent maximum velocity was 2.3 times higher for 0.1 mM-cytochrome c as substrate than for 100 mM-hydroxylamine, suggesting that the rate-limiting step during hydroxylamine reduction is a step that is not involved in cytochrome c reduction. Hydroxylamine 181-194 cytochrome c, somatic Homo sapiens 78-90 6288003-7 1982 At 0.5 mM-NAD+, the apparent maximum velocity was 2.3 times higher for 0.1 mM-cytochrome c as substrate than for 100 mM-hydroxylamine, suggesting that the rate-limiting step during hydroxylamine reduction is a step that is not involved in cytochrome c reduction. Hydroxylamine 181-194 cytochrome c, somatic Homo sapiens 239-251 6288003-8 1982 A scheme is proposed that can account for the pattern of variation with [NAD+] of the Michaelis-Menten parameters for hydroxylamine and for NADH with hydroxylamine or cytochrome c as oxidized substrate. Hydroxylamine 118-131 cytochrome c, somatic Homo sapiens 167-179 7068653-3 1982 Most of the radioactivity associated with PLP was removed when the gels were treated with hydroxylamine and then fluorographed, indicating that fatty acids were bound to PLP by ester linkage. Hydroxylamine 90-103 proteolipid protein 1 Rattus norvegicus 42-45 6291591-4 1982 Addition of hydroxylamine caused a large decrease of the 238-nm peak, which amounted to 16 mol of (ethoxyformyl)histidine/mol of cytochrome c1. Hydroxylamine 12-25 cytochrome c1 Homo sapiens 129-142 6291591-5 1982 Hydroxylamine addition to ethoxyformylated complex III also caused a small change at about 280 nm, which could be due to reversal of 1.6 O-ethoxyformylated tyrosyl residues/mol of cytochrome c1. Hydroxylamine 0-13 cytochrome c1 Homo sapiens 180-193 6977539-7 1982 The purified antithrombin-Factor IXa and antithrombin-Factor Xa complexes were dissociated by ammonia or hydroxylamine into free enzyme and a modified two-chain form of the inhibitor. Hydroxylamine 105-118 serpin family C member 1 Homo sapiens 13-25 6977539-7 1982 The purified antithrombin-Factor IXa and antithrombin-Factor Xa complexes were dissociated by ammonia or hydroxylamine into free enzyme and a modified two-chain form of the inhibitor. Hydroxylamine 105-118 serpin family C member 1 Homo sapiens 41-53 6977539-7 1982 The purified antithrombin-Factor IXa and antithrombin-Factor Xa complexes were dissociated by ammonia or hydroxylamine into free enzyme and a modified two-chain form of the inhibitor. Hydroxylamine 105-118 coagulation factor X Homo sapiens 54-63 7099146-3 1982 This reaction was demonstrated to be mediated by cytochrome P-450 as suggested by induction experiments using phenobarbital, which markedly enhanced the production of this nitroxide, and by the inhibition of this monooxygenase by metyrapone, which depressed the formation of this free radical. Hydroxylamine 172-181 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 49-65 7132955-4 1982 Previous work has shown that the 14C-labeled material spontaneously released from 14C-labeled cytochrome P-450 is in the form of oxalic acid, and that the latter is derived from a hydroxylamine-labile adduct of chloramphenicol and cytochrome P-450 [Biochem. Hydroxylamine 180-193 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 231-247 7250488-8 1981 The time course of the reaction of diethylpyrocarbonate with the estradiol receptor and the demonstration of hydroxylamine reversal of inhibition suggest that histidine is involved in the binding of estradiol receptor to oligodeoxyribonucleotides. Hydroxylamine 109-122 estrogen receptor 1 Bos taurus 199-217 7322116-4 1981 Photoregenerated rhodopsin is identical to unbleached rhodopsin in spectra and in stability to NH2OH. Hydroxylamine 95-100 rhodopsin Bos taurus 17-26 6115861-6 1981 Intermediates in the 5-oxoprolinase reaction were not detected by exchange experiments with radioactive ADP and phosphate, nor were they trapped by adding hydroxylamine. Hydroxylamine 155-168 5-oxoprolinase (ATP-hydrolysing) Rattus norvegicus 21-35 6262300-3 1981 Treatment of sodium dodecyl sulfate-polyacrylamide gels containing the [3H]palmitate-labeled transferrin receptor with hydroxylamine, prior to fluorography, resulted in release of a substantial fraction of the label from the molecule. Hydroxylamine 119-132 transferrin Homo sapiens 93-104 6262300-4 1981 In addition, at least part of the label released from immunoprecipitates of the transferrin receptor by treatment with hydroxylamine was identified as palmitohydroxamate, providing further evidence that the labeled fatty acid is covalently bound to the receptor. Hydroxylamine 119-132 transferrin Homo sapiens 80-91 6259143-9 1981 Thrombin associated with the former was released into the media when the cells were incubated at 0-4 degrees C with hirudin or hydroxylamine, or transferred to the insensitive compartment when incubated at 22 degrees C. Finally, confluent cultures of fibroblasts bind 2-3 x 10(4) 125I-alpha-thrombin molecules/cell with an apparent binding constant, i.e. Kd, of 0.7 nM (a true Kd could not be determined because of the irreversible nature of thrombin binding). Hydroxylamine 127-140 coagulation factor II, thrombin Homo sapiens 0-8 7273240-8 1981 Of the three products, only the hydroxylamine was found to bind covalently to bovine albumin. Hydroxylamine 32-45 albumin Bos taurus 85-92 7238568-5 1981 Oxidation was inhibited by the spermine oxidase inhibitors hydroxylamine and isonicotinic acid hydrazide, and inhibition of the immune response was no longer evident suggesting that the inhibitory material was a product of the action of the enzyme on spermic and spermidine. Hydroxylamine 59-72 spermine oxidase Mus musculus 31-47 6257278-0 1980 Magnetic resonance studies of apolipoprotein C-I nitroxide labeled or [13C]methyl enriched at methionine-38. Hydroxylamine 49-58 apolipoprotein C1 Homo sapiens 30-48 6257278-4 1980 As determined from its EPR spectrum, the nitroxide at Met-38 of apoC-I had a rotational correlation time (tau C) of 0.22 ns. Hydroxylamine 41-50 apolipoprotein C1 Homo sapiens 64-70 7391573-3 1980 We find that i) C4b binds covalently to cell surface components; ii) the bond between C4b and receptive molecules is hydroxylamine sensitive; iii) the alpha-polypeptide of C4b binds to receptive molecules; and iv) C5b does not interact covalently with cell surfaces. Hydroxylamine 117-130 complement C4B (Chido blood group) Homo sapiens 16-19 6968591-4 1980 254, 8516-8523] it was demonstrated that human proelastase 2 and alpha 1-protease inhibitor react slowly to form a complex that is stable to denaturation with sodium dodecyl sulfate and beta-mercaptoethanol and that the zymogen can be recovered from the isolated complex following dissociation by hydroxylamine. Hydroxylamine 297-310 serpin family A member 1 Homo sapiens 65-91 7391573-3 1980 We find that i) C4b binds covalently to cell surface components; ii) the bond between C4b and receptive molecules is hydroxylamine sensitive; iii) the alpha-polypeptide of C4b binds to receptive molecules; and iv) C5b does not interact covalently with cell surfaces. Hydroxylamine 117-130 complement C4B (Chido blood group) Homo sapiens 86-89 7391573-3 1980 We find that i) C4b binds covalently to cell surface components; ii) the bond between C4b and receptive molecules is hydroxylamine sensitive; iii) the alpha-polypeptide of C4b binds to receptive molecules; and iv) C5b does not interact covalently with cell surfaces. Hydroxylamine 117-130 complement C4B (Chido blood group) Homo sapiens 86-89 7391573-3 1980 We find that i) C4b binds covalently to cell surface components; ii) the bond between C4b and receptive molecules is hydroxylamine sensitive; iii) the alpha-polypeptide of C4b binds to receptive molecules; and iv) C5b does not interact covalently with cell surfaces. Hydroxylamine 117-130 complement C5 Homo sapiens 214-217 7445522-14 1980 The cytochrome P-450 mediated oxidation of methylformylhydrazine to a hydroxylamine derivative and further to a nitrosamide, is discussed in relation to its importance for the biological action of the hydrazine. Hydroxylamine 70-83 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 4-20 7350910-7 1980 Added hydroxylamine was cooxidized to nitrite in an amount equimolar with the HCN formed. Hydroxylamine 6-19 metastasis associated lung adenocarcinoma transcript 1 Homo sapiens 78-81 6966139-5 1980 The alpha 1-protease inhibitor-bound immunoreactive elastase 2 has been dissociated by incubation with hydroxylamine, and the resulting immunoreactive product isolated by gel filtration on Sephadex G-100. Hydroxylamine 103-116 serpin family A member 1 Homo sapiens 4-30 316698-0 1979 Carboxy terminal fragment of human alpha-1-antitrypsin from hydroxylamine cleavage: homology with antithrombin III. Hydroxylamine 60-73 serpin family A member 1 Homo sapiens 35-54 227893-5 1979 We have also demonstrated that the remote 14N of the imidazole ligand in a [14N]imidazole-heme-NO-model compound is coupled differently than in myoglobin nitroxide, demonstrating the direct effect of the protein of metal ligand bonding. Hydroxylamine 154-163 myoglobin Homo sapiens 144-153 316698-0 1979 Carboxy terminal fragment of human alpha-1-antitrypsin from hydroxylamine cleavage: homology with antithrombin III. Hydroxylamine 60-73 serpin family C member 1 Homo sapiens 98-114 618334-4 1977 The rate constant of fluorescence increase is proportional to NH2OH concentration when it is less than 0.4 M. It reaches the maximal magnitude (3.3 +/- 1 sec-1) at higher hydroxylamine concentration. Hydroxylamine 62-67 galactoside 2-alpha-L-fucosyltransferase SEC1 Bos taurus 154-159 95572-2 1979 Incubation of in vivo passaged cells, labeled with a nitroxide derivative of methyl stearate, with antiserum to membrane carried histocompatibility antigens, H-2d, resulted in an apparent decrease in membrane fluidity that was accompanied by histamine release. Hydroxylamine 53-62 histocompatibility 2, D region Mus musculus 158-162 221020-3 1979 The quenching efficiency of fatty acid spin probes is dependent on the position of the nitroxide radical group in the fatty acid chain. Hydroxylamine 87-96 spindlin 1 Homo sapiens 39-43 218926-5 1979 The linkage between H2B and ADP-Rib was rapidly hydrolyzed with 0.1 N NaOH or with 1 M neutral hydroxylamine. Hydroxylamine 95-108 histone cluster 1, H2bg Rattus norvegicus 20-35 38075-4 1979 The hydroxylamine-nitro-oxidation appears to be dependent on cytochrome P-450, as the reaction was induced by phenobarbital pretreatment and inhibited by carbon monoxide and 2,4-dichloro-6-phenylphenoxyethylamine. Hydroxylamine 4-17 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 61-77 420768-4 1979 The enzyme--antithrombin complexes could not be dissociated with sodium dodecyl sulfate (NaDodSO4) but the NaDodSO4-denatured complexes were dissociated by hydroxylamine treatment. Hydroxylamine 156-169 serpin family C member 1 Homo sapiens 12-24 207308-3 1978 The spin-labeled enzyme has been modified by urea denaturation, reduction, reduction-carboxymethylation, performic acid oxidation, and digestion with proteolytic enzymes in order to monitor changes in the geometry of the protein by changes in the electron paramagnetic resonance (EPR) spectrum of the nitroxide spin-label probe. Hydroxylamine 301-310 spindlin 1 Homo sapiens 4-8 206286-6 1978 The inhibiting effect of Cd2+ is removed in the presence of exogenous Mn2+ or hydroxylamine. Hydroxylamine 78-91 CD2 molecule Homo sapiens 25-28 274737-1 1978 The binding of a component of human complement (C1q) to membrane-bound specific anti-nitroxide antibodies was studied as a function of the physical properties (fluid vs. solid) of vesicle lipids. Hydroxylamine 85-94 complement C1q A chain Homo sapiens 48-51 45541-2 1978 The synthesis of gamma-glutamylhydroxamate from glutamate and hydroxylamine has been utilized as an approximation of glutamine synthetase activity in kidneys of rabbit, rat, dog, monkey and man. Hydroxylamine 62-75 glutamate-ammonia ligase Homo sapiens 117-137 597493-8 1977 Acta 368, 409-421), hydroxylamine destroys the secondary donor responsible for the fast reduction of Chl+. Hydroxylamine 20-33 chordin like 1 Homo sapiens 101-104 21685-4 1977 A striking difference between the catalytic activities of the rabbit and guinea pig enzymes is the low activity of rabbit transglutaminase for hydroxylamine incorporation into benzyloxycarbonyl-L-glutaminylglycine, a reaction for which the guinea pig enzyme shows a high reactivity. Hydroxylamine 143-156 protein-glutamine gamma-glutamyltransferase 2 Cavia porcellus 122-138 911824-2 1977 Incubation of chloroplasts with hydroxylamine in darkness resulted in inhibition of water oxidation and a decrease in the amplitude of cytochrome b-559 reducible by hydroquinone. Hydroxylamine 32-45 mitochondrially encoded cytochrome b Homo sapiens 135-147 911824-4 1977 Potentiometric titration of cytochrome b-559 after hydroxylamine treatment revealed a component with Em7.8 at +240 mV in addition to a lower potential species at +90 mV. Hydroxylamine 51-64 mitochondrially encoded cytochrome b Homo sapiens 28-40 911824-8 1977 In addition, the hydroxylamine light treatment caused a further change in cytochrome b-559 redox properties; a single component, Em7.8 = 90 mV is seen in titration curves. Hydroxylamine 17-30 mitochondrially encoded cytochrome b Homo sapiens 74-86 618334-4 1977 The rate constant of fluorescence increase is proportional to NH2OH concentration when it is less than 0.4 M. It reaches the maximal magnitude (3.3 +/- 1 sec-1) at higher hydroxylamine concentration. Hydroxylamine 171-184 galactoside 2-alpha-L-fucosyltransferase SEC1 Bos taurus 154-159 618334-5 1977 Fluorescence increase rate is controlled by the rate of chemical reaction of rhodopsin with hydroxylamine. Hydroxylamine 92-105 rhodopsin Bos taurus 77-86 323012-5 1977 The acylation of this single tyrosyl residue leads to the loss of the enzyme activities (hexokinase and ATPase) by a first-order process, which can be fully reversed by treatment with hydroxylamine. Hydroxylamine 184-197 hexokinase Saccharomyces cerevisiae S288C 89-99 191063-2 1977 A number of spin-labeled acyl derivatives of atractyloside, (m,n)acyl-ATR (general formula: CH3- (CH2)mCX(CH2)nCOO-ATR, where X is an o-azolidine ring containing a nitroxide), have been synthesized. Hydroxylamine 164-173 spindlin 1 Homo sapiens 12-16 191063-3 1977 As shown by electron spin resonance (ESR) spectra of spin-labeled acyl-ATR, the nitroxide placed on the acyl chain interacts with the diterpene residue of the atractyloside moiety when incorporated in liposomes. Hydroxylamine 80-89 spindlin 1 Homo sapiens 21-25 191063-3 1977 As shown by electron spin resonance (ESR) spectra of spin-labeled acyl-ATR, the nitroxide placed on the acyl chain interacts with the diterpene residue of the atractyloside moiety when incorporated in liposomes. Hydroxylamine 80-89 spindlin 1 Homo sapiens 53-57 13832-4 1977 In contrast, acetylation with acetylimidazole resulted in the conversion of all 5 tyrosine groups of lipid-free as well as lipid-bound cytochrome b5 into O-acetylated derivatives, which upon treatment with hydroxylamine were completely deacetylated. Hydroxylamine 206-219 cytochrome b5 Oryctolagus cuniculus 135-148 191815-0 1977 Surface localization of sites of reduction of nitroxide spin-labeled molecules in mitochondria. Hydroxylamine 46-55 spindlin 1 Rattus norvegicus 56-60 999850-3 1976 The influence of reduced N-methylphenazonium methosulfate (PMS) in the absence of CCCP and of oxidized PMS in the presence of CCCP on the back reaction was investigated and the following results were obtained: (1) Reduced PMS at the concentration of 1 muM inhibits the back reaction as effectively as hydroxylamine, suggesting an electron donating function of reduced PMS for System II. Hydroxylamine 302-315 proline rich protein BstNI subfamily 1 Homo sapiens 104-107 999850-3 1976 The influence of reduced N-methylphenazonium methosulfate (PMS) in the absence of CCCP and of oxidized PMS in the presence of CCCP on the back reaction was investigated and the following results were obtained: (1) Reduced PMS at the concentration of 1 muM inhibits the back reaction as effectively as hydroxylamine, suggesting an electron donating function of reduced PMS for System II. Hydroxylamine 302-315 proline rich protein BstNI subfamily 1 Homo sapiens 104-107 999850-3 1976 The influence of reduced N-methylphenazonium methosulfate (PMS) in the absence of CCCP and of oxidized PMS in the presence of CCCP on the back reaction was investigated and the following results were obtained: (1) Reduced PMS at the concentration of 1 muM inhibits the back reaction as effectively as hydroxylamine, suggesting an electron donating function of reduced PMS for System II. Hydroxylamine 302-315 proline rich protein BstNI subfamily 1 Homo sapiens 104-107 8083-8 1976 (2) NADPH-adrenodoxin reductase activity was inhibited by diethyl pyrocarbonate and the inhibition was partially reversed by addition of hydroxylamine. Hydroxylamine 137-150 ferredoxin reductase Bos taurus 10-31 1276184-8 1976 An inhibition between Z, the physiological donor and the oxidized reaction center pigment P+ occurs, proceeding as exp (-kiti)where ti is the incubation time with hydroxylamine and ki=(alpha[NH2OH]) min-1, with [NH2OH] in mM and alpha=0.14 mM-1. Hydroxylamine 163-176 CD59 molecule (CD59 blood group) Homo sapiens 199-204 1276184-8 1976 An inhibition between Z, the physiological donor and the oxidized reaction center pigment P+ occurs, proceeding as exp (-kiti)where ti is the incubation time with hydroxylamine and ki=(alpha[NH2OH]) min-1, with [NH2OH] in mM and alpha=0.14 mM-1. Hydroxylamine 191-196 CD59 molecule (CD59 blood group) Homo sapiens 199-204 217413-3 1979 These effects are all shown to be of importance in monitoring the rotational motion of carbonmonoxyhemoglobin which is spin labeled with the tightly bound nitroxide label, 4-maleimido-2,2,6,6-tetramethylpiperidinyl-1-oxy. Hydroxylamine 155-164 spindlin 1 Homo sapiens 119-123 8450-2 1976 The reaction of sulfite, cyanide, and hydroxylamine with several deazaflavin-containing enzymes (glycolate oxidase, D-amino acid oxidase, glucose oxidase, N-methylglutamate synthetase) and free deazaFMN has been examined. Hydroxylamine 38-51 hydroxyacid oxidase 2 Homo sapiens 97-114 8450-2 1976 The reaction of sulfite, cyanide, and hydroxylamine with several deazaflavin-containing enzymes (glycolate oxidase, D-amino acid oxidase, glucose oxidase, N-methylglutamate synthetase) and free deazaFMN has been examined. Hydroxylamine 38-51 D-amino acid oxidase Homo sapiens 116-136 8450-5 1976 Hydroxylamine complexes are formed with deazaFMN glycolate oxidase and deazaFAD glucose oxidase. Hydroxylamine 0-13 hydroxyacid oxidase 2 Homo sapiens 49-66 182205-3 1976 Experiments involving the translocation of ascorbate and charged nitroxide ions and the movement of paramagnetic Eu 3+ ions indicate that when ApoC-III binds to DMPC vesicles, it increases their permeability or destroys their original bilayer structure. Hydroxylamine 65-74 apolipoprotein C3 Homo sapiens 143-151 982622-2 1976 After PDH inhibilion by DEP in the presence of dithiothreitol almost complete reactivation (94%) under the effect of neutral hydroxylamine is observed. Hydroxylamine 125-138 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 6-9 178361-7 1976 When spin-labeled phosphatidylcholine, having a nitroxide on the beta chain but near the polar head-group, was incorporated into the erythrocyte membrane, ascorbate treatment at 0 degrees C allows selective reduction of the signal coming from the outer layer of the membrane. Hydroxylamine 48-57 spindlin 1 Homo sapiens 5-9 178365-4 1976 The electron spin resonance (ESR) spectra of the spin-labeled fatty acids in the macrophages showed a pronounced temperature dependence and a decrease in the hyperfine splittings (2 T11) of the spectra as the nitroxide radical was moved away from the polar head group of the fatty acid derivatives. Hydroxylamine 209-218 spindlin 1 Mus musculus 13-17 178365-4 1976 The electron spin resonance (ESR) spectra of the spin-labeled fatty acids in the macrophages showed a pronounced temperature dependence and a decrease in the hyperfine splittings (2 T11) of the spectra as the nitroxide radical was moved away from the polar head group of the fatty acid derivatives. Hydroxylamine 209-218 spindlin 1 Mus musculus 49-53 1180962-2 1975 The nature of the binding between thrombin and the inhibitor is studied by treatment of the complex with 6 M guanidinium chloride, hydroxylamine, and dilute alkali. Hydroxylamine 131-144 coagulation factor II, thrombin Homo sapiens 34-42 60792-2 1976 The nature of the binding between thrombin and the inhibitor is studied by treatment of the complex with 6 M guanidinium chloride, hydroxylamine, and dilute alkali. Hydroxylamine 131-144 coagulation factor II, thrombin Homo sapiens 34-42 60792-5 1976 Treatment of dodecyl sulfate-denatured complex with hydroxylamine results in dissociation of the complex to yield free thrombin and heparin cofactor. Hydroxylamine 52-65 coagulation factor II, thrombin Homo sapiens 119-127 1194276-3 1975 The oxygen-binding function of myoglobin, in situ in muscle fiber bundles, was abolished by treatment with nitrite of hydroxylamine, which convert oxymyoglobin in situ to high spin ferric myoglobin, or with phenylhydrazine, which converts oxymyoglobin to denatured products, or with 2-hydroxyethylhydrazine, which appears to remove myoglobin from the muslce. Hydroxylamine 118-131 myoglobin Homo sapiens 31-40 1194276-3 1975 The oxygen-binding function of myoglobin, in situ in muscle fiber bundles, was abolished by treatment with nitrite of hydroxylamine, which convert oxymyoglobin in situ to high spin ferric myoglobin, or with phenylhydrazine, which converts oxymyoglobin to denatured products, or with 2-hydroxyethylhydrazine, which appears to remove myoglobin from the muslce. Hydroxylamine 118-131 myoglobin Homo sapiens 150-159 1194276-3 1975 The oxygen-binding function of myoglobin, in situ in muscle fiber bundles, was abolished by treatment with nitrite of hydroxylamine, which convert oxymyoglobin in situ to high spin ferric myoglobin, or with phenylhydrazine, which converts oxymyoglobin to denatured products, or with 2-hydroxyethylhydrazine, which appears to remove myoglobin from the muslce. Hydroxylamine 118-131 myoglobin Homo sapiens 150-159 1191655-2 1975 Cd2+ is found to be an inhibitor on the donor side of Photosystem II and its action site, as determined by experiments using hydroxylamine and exogenous Mn, is supposed to be on the water-splitting enzyme itself. Hydroxylamine 125-138 CD2 molecule Homo sapiens 0-3 1180962-5 1975 Treatment of dodecylsulfate-denatured complex with hydroxylamine results in dissociation of the complex to yield free thrombin and heparin cofactor. Hydroxylamine 51-64 coagulation factor II, thrombin Homo sapiens 118-126 1112778-15 1975 Upon removal of the blocking groups by hydroxylamine, even the fully modified lysozyme regains complete activity. Hydroxylamine 39-52 lysozyme C, tracheal isozyme Bos taurus 78-86 240443-6 1975 The principle of this procedure is to mutate P1-transducing phage particles carrying the ATPase genes (Unc (uncoupled) DNA) using the strong chemical mutagen hydroxylamine. Hydroxylamine 158-171 ATPase Escherichia coli 89-95 239236-7 1975 These results suggest that the incorporation of the nitroxide group into drug molecules may be a useful approach to the synthesis of more specific spin labels for biological systems, such as egg white avidin, acetylcholinesterase, and the beta-adrenergic receptor. Hydroxylamine 52-61 acetylcholinesterase Rattus norvegicus 209-229 167802-1 1975 Studies are reported on nitroxide spin-labeled tropomyosin. Hydroxylamine 24-33 spindlin 1 Homo sapiens 34-38 164444-8 1975 When stearic acid derivatives bearing the nitroxide at C-5, C-12, and C-16 are bound to albumin at a ligand to albumin ratio of 1, the order of mobility at 0-30 degrees is C-16 greater than C-12 congruent to C-5. Hydroxylamine 42-51 complement C5 Homo sapiens 55-58 164444-8 1975 When stearic acid derivatives bearing the nitroxide at C-5, C-12, and C-16 are bound to albumin at a ligand to albumin ratio of 1, the order of mobility at 0-30 degrees is C-16 greater than C-12 congruent to C-5. Hydroxylamine 42-51 complement C5 Homo sapiens 208-211 1168069-3 1975 The reaction of thrombin was perturbed by addition of hydroxylamine or a competitive inhibitor and by variation of pH and it was compared with the reactions of other proteases. Hydroxylamine 54-67 coagulation factor II, thrombin Homo sapiens 16-24 33352529-3 2021 For a long time it was assumed that hydroxylamine was directly converted to nitrite by a hydroxylamine oxidoreductase. Hydroxylamine 36-49 thioredoxin reductase 1 Homo sapiens 103-117 4373731-1 1974 Three classes of lipoidal nitroxide spin probes reversibly perturb erythrocyte membranes at low concentrations (10(-10)-10(-5) M). Hydroxylamine 26-35 spindlin 1 Homo sapiens 36-40 4149766-1 1973 The use of phosphoenolpyruvate plus pyruvate kinase as an ATP-generating system in the assay for glutamine synthetase activity via the formation of gamma-glutamylhydroxamate from glutamate and hydroxylamine with crude tissue preparations is shown to give values far in excess of the true glutamine synthetase activity of the tissue. Hydroxylamine 193-206 glutamate-ammonia ligase Homo sapiens 97-117 4357553-2 1973 Selective incorporation of a nitroxide spin label sensitive to active-center geometry. Hydroxylamine 29-38 spindlin 1 Homo sapiens 39-43 4585093-0 1973 Formation of pure and mixed clones of c mutants in serratiaphage sigma after treatment of the free virion with UV or hydroxylamine. Hydroxylamine 117-130 Orofacial cleft-1 (cleft lip with or without cleft palate; isolated cleft palate) Homo sapiens 35-39 4753930-0 1973 Effect of nitrogen and of catalase on hydroxylamine and hydrazine mutagenesis. Hydroxylamine 38-51 catalase Homo sapiens 26-34 4681765-0 1972 The action of hydroxylamine on myosin. Hydroxylamine 14-27 myosin heavy chain 14 Homo sapiens 31-37 16789112-1 1969 After in vitro treatment of bacteriophage T4 with hydroxylamine (HA), 54 nonsense mutants in the rII A cistron were isolated. Hydroxylamine 50-63 protein kinase cAMP-dependent type I regulatory subunit alpha Rattus norvegicus 97-102 4307874-1 1969 The alpha-amino group of valyl-tRNA has been combined chemically with a nitroxide spin label through an amide linkage. Hydroxylamine 72-81 spindlin 1 Homo sapiens 82-86 4227782-0 1967 Hydroxylamine and a 32P-labelled intermediate in sodium-plus-potassium ion-activated adenosine triphosphatase. Hydroxylamine 0-13 ATPase Na+/K+ transporting subunit beta 1 Homo sapiens 85-109 13534735-0 1958 Rhodopsin bleaching in the presence of hydroxylamine. Hydroxylamine 39-52 rhodopsin Homo sapiens 0-9 14367777-15 1955 Hydroxylamine also bleaches iodopsin, yet does not poison its synthesis. Hydroxylamine 0-13 opsin 1 (cone pigments), long-wave-sensitive (color blindness, protan) Gallus gallus 28-36 33757896-0 2021 Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies. Hydroxylamine 0-9 spindlin 1 Homo sapiens 10-14 33929834-4 2021 This quartet state has spin polarization lifetimes longer than 0.1 ms and is characterized by relatively long coherence times of ~1.8 mus even at 80 K. Rabi oscillation experiments reveal that more than 60 single-qubit logic operations can be performed with this system at 80 K. The large magnitude of the nitroxide 14N hyperfine coupling in the quartet state of PDI-TEMPO is resolved in the transient EPR spectra and leads to a further splitting of the quartet state electron spin sublevels. Hydroxylamine 306-315 spindlin 1 Homo sapiens 23-27 33929834-4 2021 This quartet state has spin polarization lifetimes longer than 0.1 ms and is characterized by relatively long coherence times of ~1.8 mus even at 80 K. Rabi oscillation experiments reveal that more than 60 single-qubit logic operations can be performed with this system at 80 K. The large magnitude of the nitroxide 14N hyperfine coupling in the quartet state of PDI-TEMPO is resolved in the transient EPR spectra and leads to a further splitting of the quartet state electron spin sublevels. Hydroxylamine 306-315 spindlin 1 Homo sapiens 477-481 178361-4 1976 The nitroxide spin label was placed either on the beta acyl chain or on the choline group. Hydroxylamine 4-13 spindlin 1 Homo sapiens 14-18 4366759-1 1974 The lipid binding properties of the membrane protein cytochrome b(5) (detergent-extracted from calf liver microsomal preparations) were characterized by studying the interaction of spin-labeled lipids (5-, 12-, and 16-doxylstearic acid and 5- and 16-doxylphosphatidyl-choline, where doxyl refers to the nitroxide moiety) with cytochrome b(5), using electron spin resonance spectroscopy. Hydroxylamine 303-312 cytochrome b Bos taurus 53-65 11946173-0 1971 Nitroxides XLVIII: A study of the interaction between bovine serum albumin and a modified steroid by electron spin resonance. Hydroxylamine 0-10 albumin Homo sapiens 61-74 5480168-0 1970 [Inhibition of bacterial histidine decarboxylase by hydroxylamine derivatives]. Hydroxylamine 52-65 histidine decarboxylase Homo sapiens 25-48 5815704-1 1969 Treatment of highly purified preparations of the third component of complement (C3) with 0.5M hydroxylamine at 20 degrees C for 15 to 30 minutes, followed by acidification, resulted in dissociation of a peptide from the C3 molecule. Hydroxylamine 94-107 complement C3 Homo sapiens 68-82 4967774-6 1968 Cytochrome c oxidation by the Azotobacter electron transport system was markedly sensitive to cyanide, azide, and hydroxylamine, although carbon monoxide inhibition could not be demonstrated. Hydroxylamine 114-127 cytochrome c, somatic Homo sapiens 0-12 32896797-2 2020 After reaction of a nipecotic acid derivative possessing a hydroxylamine functionality with aldehydes, the resulting oxime library was screened accordingly toward the GABA transporter subtype 1 (GAT1), a drug target for several neurological disorders. Hydroxylamine 59-72 solute carrier family 6 member 1 Homo sapiens 167-193 33459015-1 2021 The nitroxide spin label is the most widely used probe for electron paramagnetic resonance (EPR) spectroscopy studies of the structure and function of biomolecules. Hydroxylamine 4-13 spindlin 1 Homo sapiens 14-18 33459015-2 2021 However, the role of surrounding environments in determining the dynamics of nitroxide spin labels in biological complex systems remains to be clarified. Hydroxylamine 77-86 spindlin 1 Homo sapiens 87-91 33012754-0 2021 Sensitive Gas Chromatography Detection of Nanomolar Hydroxylamine in Environmental Water by Fe(III) Oxidation. Hydroxylamine 52-65 gastrin Homo sapiens 10-13 33297159-8 2021 After direct attacks by PMS, they would be oxidized to form hydroxylamine-products, namely, N8-OH-TMP and N9-OH-TMP. Hydroxylamine 60-73 epithelial membrane protein 1 Homo sapiens 98-101 33297159-8 2021 After direct attacks by PMS, they would be oxidized to form hydroxylamine-products, namely, N8-OH-TMP and N9-OH-TMP. Hydroxylamine 60-73 epithelial membrane protein 1 Homo sapiens 112-115 33374857-10 2020 4-MPD additionally formed a dihydroxy metabolite and a hydroxylamine. Hydroxylamine 55-68 mevalonate diphosphate decarboxylase Homo sapiens 2-5 32896797-2 2020 After reaction of a nipecotic acid derivative possessing a hydroxylamine functionality with aldehydes, the resulting oxime library was screened accordingly toward the GABA transporter subtype 1 (GAT1), a drug target for several neurological disorders. Hydroxylamine 59-72 solute carrier family 6 member 1 Homo sapiens 195-199 32911450-7 2020 In any sample, heterogeneity is found in H-bond formation between the spin-label nitroxide groups and the solvent molecules. Hydroxylamine 81-90 spindlin 1 Homo sapiens 70-74 33171781-4 2020 METHODS: NMR (Nuclear Magnetic Resonance) and ESR (Electron Spin Resonance) spectroscopy are employed to characterize the paramagnetic perturbation of the extrinsic nitroxide probe Tempol on beta2m in the absence and presence of AuNPs to determine the surface accessibility properties and the occurrence of chemical or conformational exchange, based on measurements conducted under magnetization equilibrium and non-equilibrium conditions. Hydroxylamine 165-174 alpha-2-macroglobulin Homo sapiens 191-197 32558168-3 2020 Here we probe the interaction of the p66/p66" asymmetric reverse transcriptase precursor with HIV-1 protease by pulsed Q-band double electron-electron resonance EPR spectroscopy to measure distances between nitroxide labels introduced at surface engineered cysteine residues. Hydroxylamine 207-216 DNA polymerase delta 3, accessory subunit Homo sapiens 37-40 32558168-3 2020 Here we probe the interaction of the p66/p66" asymmetric reverse transcriptase precursor with HIV-1 protease by pulsed Q-band double electron-electron resonance EPR spectroscopy to measure distances between nitroxide labels introduced at surface engineered cysteine residues. Hydroxylamine 207-216 DNA polymerase delta 3, accessory subunit Homo sapiens 41-44 33247742-8 2020 CONCLUSIONS: The degradation products were identified as benzimidazole (DP1) and amine N-oxide of bilastine (DP2). Hydroxylamine 81-94 transcription factor Dp-2 Homo sapiens 109-112 32970420-0 2020 Hydroxylamine Complexes of Cytochrome c": Influence of Heme Iron Redox State on Kinetic and Spectroscopic Properties. Hydroxylamine 0-13 D-alanyl-D-alanine carboxypeptidase Achromobacter xylosoxidans 27-39 32561320-0 2020 Unexpected rapid aerobic transformation of 2,2,6,6-tetraethyl-4-oxo(piperidin-1-yloxyl) radical by cytochrome P450 in the presence of NADPH: evidence against a simple reduction of the nitroxide moiety to the hydroxylamine. Hydroxylamine 184-193 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 99-114 32869045-1 2020 We report the observation of electron spin polarization transfer from the triplet state of a porphyrin to a weakly coupled nitroxide radical in a mutant of human neuroglobin (NGB). Hydroxylamine 123-132 neuroglobin Homo sapiens 162-173 32869045-1 2020 We report the observation of electron spin polarization transfer from the triplet state of a porphyrin to a weakly coupled nitroxide radical in a mutant of human neuroglobin (NGB). Hydroxylamine 123-132 neuroglobin Homo sapiens 175-178 32869045-2 2020 The native iron-containing heme substrate of NGB has been substituted with Zn(ii) protoporphyrin IX and the nitroxide has been attached via site-directed spin labeling to the Cys120 residue. Hydroxylamine 108-117 neuroglobin Homo sapiens 45-48 32561320-0 2020 Unexpected rapid aerobic transformation of 2,2,6,6-tetraethyl-4-oxo(piperidin-1-yloxyl) radical by cytochrome P450 in the presence of NADPH: evidence against a simple reduction of the nitroxide moiety to the hydroxylamine. Hydroxylamine 208-221 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 99-114 32366855-1 2020 Tempol (4-hydroxy-2,2,6,6-Tetramethylpiperidine-1-oxyl, TPL), a nitroxide compound, inhibits proliferation and increases the vulnerability of cancer cells to apoptosis induced by cytotoxic agents. Hydroxylamine 64-73 BPI fold containing family A, member 5 Mus musculus 56-59 32436700-0 2020 The Underlying Mechanism of HNO Production by the Myoglobin-Mediated Oxidation of Hydroxylamine. Hydroxylamine 82-95 myoglobin Homo sapiens 50-59 32436700-3 2020 In this study, we examined the ability of myoglobin to produce HNO via the peroxidation of hydroxylamine with H2O2 using both experimental and computational approaches. Hydroxylamine 91-104 myoglobin Homo sapiens 42-51 32436700-9 2020 Our results confirm that myoglobin produces HNO via the peroxidation of hydroxylamine with a great catalytic capacity. Hydroxylamine 72-85 myoglobin Homo sapiens 25-34 32608797-4 2020 Under the same hydroxylamine concentration (HA=5 mg L-1) at a higher pH environment (pH >= 7.5), hydroxylamine produced more free hydroxylamine (FHA) and the inhibitory effect on Nitrobacter was improved. Hydroxylamine 97-110 L1 cell adhesion molecule Homo sapiens 52-55 32608797-4 2020 Under the same hydroxylamine concentration (HA=5 mg L-1) at a higher pH environment (pH >= 7.5), hydroxylamine produced more free hydroxylamine (FHA) and the inhibitory effect on Nitrobacter was improved. Hydroxylamine 97-110 L1 cell adhesion molecule Homo sapiens 52-55 32608797-7 2020 When pH=7.5 and hydroxylamine concentration was 45 mg L-1, the relative activity of Nitrospira was 82%. Hydroxylamine 16-29 L1 cell adhesion molecule Homo sapiens 54-57 32298086-3 2020 Reactive oxygen species (ROS) including OH, HO2 and 1O2 are proposed to be the main oxidants of the PI/HA system, which is supported by various tests employing the scavengers, chemical probes, and spin-trapping electron paramagnetic resonance (EPR) technique. Hydroxylamine 105-107 heme oxygenase 2 Homo sapiens 45-57 32222539-1 2020 Nitroxides and nitroxide-containing nanoparticles (RNP) are excellent antioxidants. Hydroxylamine 15-24 RNA binding region (RNP1, RRM) containing 3 Homo sapiens 51-54 32276437-1 2020 Four albumin-nitroxide conjugates were prepared and tested as metal-free organic radical contrast agents (ORCAs) for magnetic resonance imaging (MRI). Hydroxylamine 13-22 serum albumin Orcinus orca 5-12 32103670-6 2020 The partial reduction of ECm during RNA synthesis was circumvented by the protection of the nitroxide as a benzoylated hydroxylamine. Hydroxylamine 92-101 multimerin 1 Homo sapiens 25-28 32103670-6 2020 The partial reduction of ECm during RNA synthesis was circumvented by the protection of the nitroxide as a benzoylated hydroxylamine. Hydroxylamine 119-132 multimerin 1 Homo sapiens 25-28 31196425-5 2019 Under optimized conditions, the fabricated sensor displays two wide linear responses in the ranges of 5.8-733.8 muM and 733.8-2933.8 muM for hydroxylamine, with a rapid response time about 1 s. For chlorogenic acid, the sensor presents linear responses in the ranges of 0.1-2 muM and 2-15 muM, with a high sensitivity of 10.18 muA/muM. Hydroxylamine 141-154 latexin Homo sapiens 112-115 32155745-0 2020 Hydroxylamine Analogue of Agmatine: Magic Bullet for Arginine Decarboxylase. Hydroxylamine 0-13 antizyme inhibitor 2 Homo sapiens 53-75 32155745-4 2020 Here we demonstrate that the isosteric hydroxylamine analogue of agmatine (AO-Agm) is a new and very potent (IC50 3 10-8 M) inhibitor of E. coli ADC. Hydroxylamine 39-52 antizyme inhibitor 2 Homo sapiens 145-148 31907178-2 2020 Hydroxylamine (HYD) and carboxymethoxylamine (CAR) have been reported as inhibitors of aspartate aminotransferases (AATs), and interferes with the proliferation in Plasmodium falciparum Therefore, AATs are suggested as drug targets against Plasmodium T. gondii genome encodes only one predicted AAT in both T. gondii type I RH and type II PLK strains. Hydroxylamine 0-13 serine (or cysteine) preptidase inhibitor, clade A, member 1B Mus musculus 116-119 31907178-2 2020 Hydroxylamine (HYD) and carboxymethoxylamine (CAR) have been reported as inhibitors of aspartate aminotransferases (AATs), and interferes with the proliferation in Plasmodium falciparum Therefore, AATs are suggested as drug targets against Plasmodium T. gondii genome encodes only one predicted AAT in both T. gondii type I RH and type II PLK strains. Hydroxylamine 0-13 polo like kinase 1 Mus musculus 339-342 31907178-2 2020 Hydroxylamine (HYD) and carboxymethoxylamine (CAR) have been reported as inhibitors of aspartate aminotransferases (AATs), and interferes with the proliferation in Plasmodium falciparum Therefore, AATs are suggested as drug targets against Plasmodium T. gondii genome encodes only one predicted AAT in both T. gondii type I RH and type II PLK strains. Hydroxylamine 15-18 serine (or cysteine) preptidase inhibitor, clade A, member 1B Mus musculus 116-119 31907178-2 2020 Hydroxylamine (HYD) and carboxymethoxylamine (CAR) have been reported as inhibitors of aspartate aminotransferases (AATs), and interferes with the proliferation in Plasmodium falciparum Therefore, AATs are suggested as drug targets against Plasmodium T. gondii genome encodes only one predicted AAT in both T. gondii type I RH and type II PLK strains. Hydroxylamine 15-18 polo like kinase 1 Mus musculus 339-342 31907178-7 2020 All these indicated that HYD and CAR inhibiting T. gondii growth and controlling toxoplasmosis can occur in an AAT-independent pathway. Hydroxylamine 25-28 serine (or cysteine) preptidase inhibitor, clade A, member 1B Mus musculus 111-114 31667988-3 2020 Incubations using pS9 led to the formation of an acetyl conjugation in case of 2-AI and merely a hydroxylamine for NM-2-AI. Hydroxylamine 97-110 taste 2 receptor member 2, pseudogene Homo sapiens 18-21 31494687-9 2019 Thanks to the use of silver NPs prepared by reduction of silver nitrate with hydroxylamine hydrochloride, SP can be detected at very low peptide concentration (~ 90 nM). Hydroxylamine 77-104 tachykinin precursor 1 Homo sapiens 106-108 32213406-7 2020 In BAF2, N2O was emitted from AOB denitrification and hydroxylamine oxidation by 87.8% and 12.2%, respectively. Hydroxylamine 54-67 BANF family member 2 Homo sapiens 3-7 31196425-5 2019 Under optimized conditions, the fabricated sensor displays two wide linear responses in the ranges of 5.8-733.8 muM and 733.8-2933.8 muM for hydroxylamine, with a rapid response time about 1 s. For chlorogenic acid, the sensor presents linear responses in the ranges of 0.1-2 muM and 2-15 muM, with a high sensitivity of 10.18 muA/muM. Hydroxylamine 141-154 latexin Homo sapiens 133-136 31196425-5 2019 Under optimized conditions, the fabricated sensor displays two wide linear responses in the ranges of 5.8-733.8 muM and 733.8-2933.8 muM for hydroxylamine, with a rapid response time about 1 s. For chlorogenic acid, the sensor presents linear responses in the ranges of 0.1-2 muM and 2-15 muM, with a high sensitivity of 10.18 muA/muM. Hydroxylamine 141-154 latexin Homo sapiens 133-136 31196425-5 2019 Under optimized conditions, the fabricated sensor displays two wide linear responses in the ranges of 5.8-733.8 muM and 733.8-2933.8 muM for hydroxylamine, with a rapid response time about 1 s. For chlorogenic acid, the sensor presents linear responses in the ranges of 0.1-2 muM and 2-15 muM, with a high sensitivity of 10.18 muA/muM. Hydroxylamine 141-154 latexin Homo sapiens 133-136 31196425-5 2019 Under optimized conditions, the fabricated sensor displays two wide linear responses in the ranges of 5.8-733.8 muM and 733.8-2933.8 muM for hydroxylamine, with a rapid response time about 1 s. For chlorogenic acid, the sensor presents linear responses in the ranges of 0.1-2 muM and 2-15 muM, with a high sensitivity of 10.18 muA/muM. Hydroxylamine 141-154 latexin Homo sapiens 133-136 30936015-6 2019 However, it was released by incubation with 1 M neutral NH2OH or 0.1 N NaOH, but was not with 0.1 N HCl, suggesting the bond between ADH1 and poly(ADP-ribose) is an ester linkage. Hydroxylamine 56-61 alcohol dehydrogenase 1A (class I), alpha polypeptide Homo sapiens 133-137 30817078-2 2019 Here we employ orthogonal spin labels, a chromophore triplet state and a stable radical, to carry out distance measurements in singly nitroxide-labeled human neuroglobin. Hydroxylamine 134-143 neuroglobin Homo sapiens 158-169 31441130-4 2019 Here, we have used hydroxylamine-based random mutagenesis to identify amino acids important for Pcp1p peptidase activity. Hydroxylamine 19-32 rhomboid protease PCP1 Saccharomyces cerevisiae S288C 96-101 31251620-1 2019 A model-free approach for simulation of EPR spectra of nitroxide spin probes in liquid-crystalline materials was suggested and used to obtain parameters of molecular orientation and rotational mobility. Hydroxylamine 55-64 spindlin 1 Homo sapiens 65-69 31012470-7 2019 We discovered that in both KS1-2 and DDR2, the hydroxylamine reaction is light accelerated, even in artificial pigments derived from synthetic retinal in which the crucial C13[double bond, length as m-dash]C14 double-bond isomerization is prevented. Hydroxylamine 47-60 zinc finger protein 382 Homo sapiens 27-32 31012470-7 2019 We discovered that in both KS1-2 and DDR2, the hydroxylamine reaction is light accelerated, even in artificial pigments derived from synthetic retinal in which the crucial C13[double bond, length as m-dash]C14 double-bond isomerization is prevented. Hydroxylamine 47-60 discoidin domain receptor tyrosine kinase 2 Homo sapiens 37-41 30768266-0 2019 Comment on "Distinct Populations in Spin-Label EPR Spectra from Nitroxides". Hydroxylamine 64-74 spindlin 1 Homo sapiens 36-40 30776218-3 2019 A protein homodimer, TRIM25cc, was selectively labeled with [Gd.sTPATCN]-SL (70%) and a nitroxide (30%) under mild conditions and measured using the double electron electron resonance (DEER) technique with both commercial Q-band and home-built W-band spectrometers. Hydroxylamine 88-97 tripartite motif containing 25 Homo sapiens 21-27 30620548-7 2019 PRE-NMR distances obtained from a nitroxide spin-label at Cys84 showed that the L29Q mutation perturbs the structure of the TnC N-domain in the presence and absence of Ca2+, with a more "open" TnC N-domain observed in the apo form. Hydroxylamine 34-43 tenascin C Homo sapiens 124-127 30500095-0 2019 Semi-Rigid Nitroxide Spin Label for Long-Range EPR Distance Measurements of Lipid Bilayer Embedded beta-Peptides. Hydroxylamine 11-20 spindlin 1 Homo sapiens 21-25 30468545-0 2019 Synthesis of mu2 -Oxo-Bridged Iron(III) Tetraphenylporphyrin-Spacer-Nitroxide Dimers and their Structural and Dynamics Characterization by using EPR and MD Simulations. Hydroxylamine 68-77 adaptor related protein complex 1 subunit mu 2 Homo sapiens 13-16 30737560-0 2019 Spin polarization in graphene nanoribbons functionalized with nitroxide. Hydroxylamine 62-71 spindlin 1 Homo sapiens 0-4 30737560-3 2019 Such interacting nitroxide groups induce spin polarization of the edge states of stable graphene nanoribbons. Hydroxylamine 17-26 spindlin 1 Homo sapiens 41-45 30737560-4 2019 Graphical abstract Exchange coupling constants inducing spin polarization in graphene nanoribbons functionalized with nitroxides. Hydroxylamine 118-128 spindlin 1 Homo sapiens 56-60 30602386-12 2019 Repeated injection of NaHS enhanced CBS and TRPC6 expression, but hydroxylamine (HA) prevented the STZ-induced increase of CBS protein. Hydroxylamine 66-79 cystathionine beta synthase Rattus norvegicus 123-126 30688300-3 2019 Cyclic hydroxylamine spin probes react selectively with superoxide or other radicals to generate a nitroxide signal that can be quantified by EPR spectroscopy. Hydroxylamine 99-108 spindlin 1 Homo sapiens 21-25 30602386-12 2019 Repeated injection of NaHS enhanced CBS and TRPC6 expression, but hydroxylamine (HA) prevented the STZ-induced increase of CBS protein. Hydroxylamine 81-83 cystathionine beta synthase Rattus norvegicus 123-126 30222354-2 2018 Paramagnetic metal ions, such as Cu2+, are used as spin probes because they can report on metalloprotein features and can be spectroscopically distinguished from traditional nitroxide (NO)-based labels. Hydroxylamine 174-183 spindlin 1 Homo sapiens 51-55 31950744-7 2019 The formation and stability of nanoflowers and nanospheres for PMR and PML with hydroxylamine-based reduction were further studied in detail with diverse controlled amine-modified (5"-, 3"- and both end-modified) and non-modified DNA sequences with other mutants of these two sequences. Hydroxylamine 80-93 PML nuclear body scaffold Homo sapiens 71-74 30577733-0 2018 Effects of NaHS and hydroxylamine on the expressions of brain-derived neurotrophic factor and its receptors in rats after cardiac arrest and cardiopulmonary resuscitation. Hydroxylamine 20-33 brain-derived neurotrophic factor Rattus norvegicus 56-89 30539955-1 2018 A cascade or domino sequence of condensation of hydroxylamine and an aldehyde to give an oxime, cyclization to a nitrone, and intramolecular 1,3-dipolar cycloaddition has been successfully employed where there is branching at C-4 as a route to the iboga alkaloids. Hydroxylamine 48-61 complement C4A (Rodgers blood group) Homo sapiens 226-229 30192035-3 2018 The calculations confirm the presence of temperature-dependent exchange interaction within the spin triads formed by the nitroxide-copper(II)-nitroxide units, in line with the changes observed in the effective magnetic moment. Hydroxylamine 121-130 spindlin 1 Homo sapiens 95-99 30192035-4 2018 Moreover, they quantify the interchain interaction mediated by the terminal nitroxide group of two spin triads in neighboring polymer chains. Hydroxylamine 76-85 spindlin 1 Homo sapiens 99-103 30373764-5 2018 The catalytic activity of TIPARP was resistant to meta-iodobenzylguanidine but sensitive to iodoacetamide and hydroxylamine, implicating cysteines and acidic side chains as ADP-ribosylated target residues. Hydroxylamine 110-123 TCDD inducible poly(ADP-ribose) polymerase Homo sapiens 26-32 30686862-3 2018 Here we present a method that allows for spin-labeling of protein nucleotide binding sites by adenosine diphosphate (ADP) modified with a nitroxide moiety on the beta-phosphate (ADP-beta-S-SL). Hydroxylamine 138-147 spindlin 1 Homo sapiens 41-45 30035527-11 2018 We also reiterate how their impressive RTA activity translates from solution to mammalian cell culture, wherein we have demonstrated that diarylamines and their derived nitroxides are potent inhibitors of ferroptosis, a recently characterized form of cell death associated with lipid peroxidation (autoxidation). Hydroxylamine 169-179 MAS related GPR family member F Homo sapiens 39-42 29577465-3 2018 The light-responsive spin-silenced polymer is synthesized via an Ugi post-polymerization modification incorporating paramagnetic nitroxides and a light cleavable fluorophore moiety. Hydroxylamine 129-139 spindlin 1 Homo sapiens 21-25 29131419-4 2018 Classes that have been developed as SOD mimetics include the Mn-metalloporphyrins, Mn-cyclic polyamines, Mn-salen complexes, MnPLED derivatives as well as the nitroxides. Hydroxylamine 159-169 superoxide dismutase 2 Homo sapiens 36-39 29722459-1 2018 A nitroxide carrying a peptide specific to the binding pocket of the serine proteases chymotrypsin and cathepsin G is prepared. Hydroxylamine 2-11 cathepsin G Homo sapiens 103-114 29037084-10 2018 FUTURE DIRECTIONS: Additional studies to advance hydroxylamine spin probes from the "basic science" to biomedical applications are needed and could lead to better understanding of pathological conditions associated with oxidative stress. Hydroxylamine 49-62 spindlin 1 Homo sapiens 63-67 29369431-5 2018 To render a particular target molecule accessible for PELDOR, it can be engineered to contain only one or two surface-exposed cysteine residues, which can be efficiently spin-labelled using thiol-reactive nitroxide compounds. Hydroxylamine 205-214 spindlin 1 Homo sapiens 170-174 29614227-2 2018 This ESEEM method detects dipolar couplings between 2H-labeled nuclei on the side chains of an amino acid (Leu or Val) and a strategically placed nitroxide spin-label in the proximity up to 8 A. ESEEM spectra patterns for different samples correlate directly to the periodic structural feature of different secondary structures. Hydroxylamine 146-155 spindlin 1 Homo sapiens 156-160 29485736-0 2018 A Cytidine Phosphoramidite with Protected Nitroxide Spin Label: Synthesis of a Full-Length TAR RNA and Investigation by In-Line Probing and EPR Spectroscopy. Hydroxylamine 42-51 RNA binding motif protein 8A Homo sapiens 91-94 29485736-1 2018 EPR studies on RNA are complicated by three major obstacles related to the chemical nature of nitroxide spin labels: Decomposition while oligonucleotides are chemically synthesized, further decay during enzymatic strand ligation, and undetected changes in conformational equilibria due to the steric demand of the label. Hydroxylamine 94-103 spindlin 1 Homo sapiens 104-108 29485736-3 2018 By careful selection of ligation sites and splint oligonucleotides, high yields were achieved in the assembly of a full-length HIV-1 TAR RNA labeled with two protected nitroxide groups. Hydroxylamine 168-177 RNA binding motif protein 8A Homo sapiens 133-136 29775060-0 2018 Distinct Populations in Spin-Label EPR Spectra from Nitroxides. Hydroxylamine 52-62 spindlin 1 Homo sapiens 24-28 29775060-1 2018 Two-component nitroxide spin-label electron paramagnetic presonance (EPR) spectra are important to the analysis of lipid-protein interactions, phase separation in lipid membranes, and conformational changes in proteins. Hydroxylamine 14-23 spindlin 1 Homo sapiens 24-28 29796455-5 2018 Simulated STM images suggested the change in electron density that would occur upon adsorption of hydroxylamine in various adsorption configurations, and specifically indicated the N-O dissociative product to have greater electron density around the amine groups, and the hydroxyl groups to mainly contribute electron density to the unoccupied electronic states. Hydroxylamine 98-111 sulfotransferase family 1A member 3 Homo sapiens 10-13 29522712-9 2018 A glutathione trapping assay revealed the formation of hydroxylamine via an AOX1-dependent reduction of dantrolene but not via hydroxylation of aminodantrolene. Hydroxylamine 55-68 aldehyde oxidase 1 Homo sapiens 76-80 29451786-5 2018 Inhibited autoxidations of THF in aqueous buffers reveal that nitroxides reduce peroxyl radicals by electron transfer with rate constants ( k 106 to >107 M-1 s-1) that correlate with the standard potentials of the nitroxides ( E 0.75-0.95 V vs NHE) and that this activity is catalytic in nitroxide. Hydroxylamine 62-71 solute carrier family 9 member C1 Homo sapiens 252-255 29896379-6 2018 Moreover, the J values of TNL1/TNL2 with the S configuration in the nitroxide part vary with temperature and the polarity of solvents due to their flexible linker, whereas the J values of TNT1/TNT2 are almost insensitive to these two factors due to the rigidity of their linkers. Hydroxylamine 68-77 tripartite motif containing 67 Homo sapiens 26-29 29451325-0 2018 Purine-Derived Nitroxides for Noncovalent Spin-Labeling of Abasic Sites in Duplex Nucleic Acids. Hydroxylamine 15-25 spindlin 1 Homo sapiens 42-46 29451786-5 2018 Inhibited autoxidations of THF in aqueous buffers reveal that nitroxides reduce peroxyl radicals by electron transfer with rate constants ( k 106 to >107 M-1 s-1) that correlate with the standard potentials of the nitroxides ( E 0.75-0.95 V vs NHE) and that this activity is catalytic in nitroxide. Hydroxylamine 62-72 solute carrier family 9 member C1 Homo sapiens 252-255 29513743-3 2018 Nitroxide spin-label probes were introduced at individual GCAP1 residues: T29C, E57C, E133C, and E154C. Hydroxylamine 0-9 guanylate cyclase activator 1A Homo sapiens 58-63 29391546-2 2018 In the current study, we demonstrate that a mitochondrion-targeted nitroxide JP4-039 given once 24 hours after 9-10 Gy TBI significantly improves mouse survival, and the recovery of intestinal barrier, differentiation and stem cell functions. Hydroxylamine 67-76 junctophilin 4 Mus musculus 77-80 29098905-3 2018 Nitroxides containing acetoxymethoxycarbonyl groups were rapidly absorbed by cells from the media, 3,4-bis-(acetoxymethoxycarbonyl)-proxyl (DCP-AM2) and 3-(2-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)acetamido)-proxyl (DCAP-AM2) showing the strongest EPR signal of the cellular fraction. Hydroxylamine 0-10 adrenomedullin 2 Mus musculus 144-147 29098905-3 2018 Nitroxides containing acetoxymethoxycarbonyl groups were rapidly absorbed by cells from the media, 3,4-bis-(acetoxymethoxycarbonyl)-proxyl (DCP-AM2) and 3-(2-(bis(2-(acetoxymethoxy)-2-oxoethyl)amino)acetamido)-proxyl (DCAP-AM2) showing the strongest EPR signal of the cellular fraction. Hydroxylamine 0-10 adrenomedullin 2 Mus musculus 223-226 29098905-9 2018 Moreover, administration of DCP-AM2 to mice (1.4 mg/kg/day) resulted in substantial nitroxide accumulation in the tissues and significantly reduced hypertension. Hydroxylamine 84-93 adrenomedullin 2 Mus musculus 32-35 29414705-1 2018 Nitroxide- and Cu2+-based electron spin resonance (ESR) are combined to provide insight into the conformational states of the functionally important alpha-helix of the human glutathione S-transferase A1. Hydroxylamine 0-9 glutathione S-transferase alpha 1 Homo sapiens 174-202 29610552-7 2018 We found that nitroxides reduced caspase-3 activation and may have ultimately inhibited cell death. Hydroxylamine 14-24 caspase 3 Homo sapiens 33-42 29272914-6 2018 The resulting beacon uses fluorine (19F) as a reporter, which is broadened, or turned "off", via paramagnetic relaxation enhancement from a stabilized nitroxide radical spin label when the beacon is not bound to its nucleic acid target. Hydroxylamine 151-160 ubiquitin like 5 Homo sapiens 14-20 29272914-6 2018 The resulting beacon uses fluorine (19F) as a reporter, which is broadened, or turned "off", via paramagnetic relaxation enhancement from a stabilized nitroxide radical spin label when the beacon is not bound to its nucleic acid target. Hydroxylamine 151-160 ubiquitin like 5 Homo sapiens 189-195 29251492-1 2018 The configurational space sampled by the finger and thumb subdomains of the p66 subunit of HIV-1 reverse transcriptase was investigated by Q-band double electron-electron resonance pulsed electron paramagnetic resonance spectroscopy, a method for determining long-range distances between pairs of nitroxide spin-labels introduced via surface-engineered cysteine residues. Hydroxylamine 297-306 DNA polymerase delta 3, accessory subunit Homo sapiens 76-79 29227566-0 2018 A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies. Hydroxylamine 15-24 spindlin 1 Homo sapiens 25-29 29227566-3 2018 Until now, the use of nitroxide-based spin labels for in-cell studies has represented a major hurdle because of their short persistence in the cellular context. Hydroxylamine 22-31 spindlin 1 Homo sapiens 38-42 29034946-4 2017 In this work, we compare different types of nitroxide-based and Gd(iii)-based spin labels attached to isolated RBDs of the polypyrimidine-tract binding protein 1 (PTBP1) and to short RNA fragments. Hydroxylamine 44-53 polypyrimidine tract binding protein 1 Homo sapiens 123-161 29313041-1 2018 Orientation selective (OS) RIDME and PELDOR were conducted on a low-spin CoII complex coordinated by two nitroxide (NO) labelled 2,2":6",2""-terpyridine ligands. Hydroxylamine 105-114 mitochondrially encoded cytochrome c oxidase II Homo sapiens 73-77 29034946-4 2017 In this work, we compare different types of nitroxide-based and Gd(iii)-based spin labels attached to isolated RBDs of the polypyrimidine-tract binding protein 1 (PTBP1) and to short RNA fragments. Hydroxylamine 44-53 polypyrimidine tract binding protein 1 Homo sapiens 163-168 28812076-2 2017 The combination of [WZn3(H2O)2(ZnW9O34)2]12- and Co(ii) provides a synergistical catalytic way to promote oximation of aldehyde/ketone with in situ generated hydroxylamine that initially produces an oxime, which further either dehydrates into a nitrile or undergoes a Beckmann rearrangement to form an amide. Hydroxylamine 158-171 mitochondrially encoded cytochrome c oxidase II Homo sapiens 49-55 28944812-4 2017 The thermochromism of these complexes evident upon thermal spin state switching is mainly caused by a spectral shift of the absorption bands of the nitroxides. Hydroxylamine 148-158 spindlin 1 Homo sapiens 59-63 28877484-2 2017 We compare the computationally generated structural ensembles of the IDP amyloid-beta42 (Abeta42) to an alternative sequence in which a nitroxide spin label attached to cysteine has been introduced at its N-terminus. Hydroxylamine 136-145 spindlin 1 Homo sapiens 146-150 28771001-3 2017 Here we demonstrate that mTG can efficiently couple three different acyl-acceptor molecules, l-lysine, glycine ethyl ester, and hydroxylamine, to gliadin peptides and protein. Hydroxylamine 128-141 protease, serine 3 Mus musculus 25-28 28570782-2 2017 Electron paramagnetic resonance (EPR) of biomolecules spin-labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin-labeling strategies are needed. Hydroxylamine 72-82 spindlin 1 Homo sapiens 54-58 28344126-8 2017 Tempol and related nitroxides decreased NO consumption in ascorbate-replete fluids by scavenging MPO-derived ascorbyl radicals. Hydroxylamine 19-29 myeloperoxidase Homo sapiens 97-100 27321253-14 2017 These results suggest that the ability of benzocaine to form MetHb is likely to be mediated through its hydroxylamine metabolite and that this metabolite is inherently more active than the potentially MetHb-forming metabolites of lidocaine. Hydroxylamine 104-117 hemoglobin subunit gamma 2 Homo sapiens 61-66 28295910-5 2017 Using this oxidizing agent, DEER distance measurements are performed on doubly nitroxide-labeled GB1, the immunoglobulin-binding domain of protein G, at varying incubation times in the cellular environment. Hydroxylamine 79-88 gamma-aminobutyric acid type B receptor subunit 1 Homo sapiens 97-100 28569901-4 2017 We studied the conformational transition of the nitroxide labeled, isolated carboxy-terminal cyclic-nucleotide binding domain (CNBD) of the HCN2 ion channel upon binding of the ligand 3",5"-cyclic adenosine monophosphate (cAMP). Hydroxylamine 48-57 hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2 Homo sapiens 140-144 27342129-0 2017 Nitroxide Spin-Labelling and Its Role in Elucidating Cuproprotein Structure and Function. Hydroxylamine 0-9 spindlin 1 Homo sapiens 10-14 27342129-3 2017 When spin-labels such as aminoxyl radicals (commonly referred to as nitroxides) are introduced, then EPR becomes a powerful technique to monitor not only the coordination environment, but also to obtain structural information that is often not readily available from other techniques. Hydroxylamine 68-78 spindlin 1 Homo sapiens 5-9 27342129-5 2017 The theory and practice of EPR can be daunting to the non-expert; therefore, in this mini review, we explore how nitroxide spin-labelling can be used to help the inorganic biochemist gain greater understanding of cuproprotein structure and function in vitro and how EPR imaging may help improve understanding of copper homoeostasis in vivo. Hydroxylamine 113-122 spindlin 1 Homo sapiens 123-127 28512115-9 2017 In contrast, hydroxylamine significantly increased the systolic blood pressure of Wistar-Kyoto rats, along with decreased carotid sinus baroreceptor sensitivity and reduced TRPV1 protein expression in the carotid sinus. Hydroxylamine 13-26 transient receptor potential cation channel, subfamily V, member 1 Rattus norvegicus 173-178 28532685-3 2017 The purpose of this paper is to address the inhibition of protein disulfide isomerase (PDI), an essential redox chaperone whose active sites contain the Cys-Gly-His-Cys (CXXC) motif, by the nitroxide Tempol. Hydroxylamine 190-199 prolyl 4-hydroxylase, beta polypeptide Mus musculus 58-85 28532685-3 2017 The purpose of this paper is to address the inhibition of protein disulfide isomerase (PDI), an essential redox chaperone whose active sites contain the Cys-Gly-His-Cys (CXXC) motif, by the nitroxide Tempol. Hydroxylamine 190-199 prolyl 4-hydroxylase, beta polypeptide Mus musculus 87-90 28532685-11 2017 CONCLUSION: We have shown that the nitroxide Tempol acts as an inhibitor of PDI through covalent binding to the Cys400 of the protein structure. Hydroxylamine 35-44 prolyl 4-hydroxylase, beta polypeptide Mus musculus 76-79 28352902-2 2017 Poly(methyl methacrylate)-grad-(styrene) (PMMA-grad-PSt) copolymers were synthesized in semi-batch mode using nitroxide-mediated polymerization (NMP) with varied monomer injection protocols to produce varied diffuse interfaces (number average molecular weights (Mn) ranged from 62 000 g mol-1 to 94 000 g mol-1 with dispersities (D) between 1.35 and 1.59). Hydroxylamine 110-119 sulfotransferase family 1A member 1 Homo sapiens 52-55 28339206-4 2017 In this study, 2H-labeled d10-leucine (Leu) was substituted at a specific Leu residue (i) and a nitroxide spin label was positioned 2, 3, and 4 residues away (denoted i+2 to i+4) on an amphipathic model peptide, LRL8. Hydroxylamine 96-105 spindlin 1 Homo sapiens 106-110 27789204-5 2016 Concomitantly, MPO activity increased in left ventricular (LV) homogenates prepared from the AMI group and this was inhibited by paracetamol and the nitroxide TEMPO. Hydroxylamine 149-158 myeloperoxidase Rattus norvegicus 15-18 28890500-8 2017 In the chronoamperometric detection of hydroxylamine, the sensor exhibited a detection limit of 0.80 muM (S/N = 3) and a high sensitivity of 184 muA mM-1 cm-2. Hydroxylamine 39-52 latexin Homo sapiens 101-104 27911291-8 2017 In this study, we provide the first demonstration that a nitroxide-based small-molecule produces MRI contrast in brain specimens with elevated levels of Abeta. Hydroxylamine 57-66 amyloid beta (A4) precursor protein Mus musculus 153-158 29732238-9 2017 We identified initial signature of simvastatin-induced insulin resistance, including ethanolamine, hydroxylamine, hydroxycarbamate and isoleucine which, upon further replication and expansion, could be predictive biomarkers of individual susceptibility to simvastatin-induced new onset pre-type II diabetes mellitus. Hydroxylamine 99-112 insulin Homo sapiens 55-62 27908452-9 2017 This study concludes that NO3- formation during ozonation of DON is induced by an oxygen-transfer to nitrogen forming hydroxylamine and oxime, while NH4+ formation is induced by electron-transfer reactions involving C-centered radicals and imine intermediates. Hydroxylamine 118-131 NBL1, DAN family BMP antagonist Homo sapiens 26-29 27998079-0 2016 Designing Molecular Probes To Prolong Intracellular Retention: Application to Nitroxide Spin Probes. Hydroxylamine 78-87 spindlin 1 Homo sapiens 88-92 27189101-1 2016 We report the synthesis and characterisation of the nitroxide spin-labelled photoactivatable Pt(iv) prodrug trans,trans,trans-[Pt(N3)2(OH)(OCOCH2CH2CONH-TEMPO)(Py)2] (Pt-TEMPO, where TEMPO = 2,2,6,6-tetramethylpiperidine 1-oxyl). Hydroxylamine 52-61 spindlin 1 Homo sapiens 62-66 27661977-1 2016 Reduction of hydroxylamine to ammonium by phytoglobin, a plant hexacoordinate hemoglobin, is much faster than that of other hexacoordinate hemoglobins or pentacoordinate hemoglobins such as myoglobin, leghemoglobin, and red blood cell hemoglobin. Hydroxylamine 13-26 leghemoglobin A Glycine max 201-214 27661977-3 2016 Experiments were conducted with phytoglobins from rice, tomato, and soybean along with human neuroglobin and soybean leghemoglobin that reveal hydroxylamine binding as the rate-limiting step. Hydroxylamine 143-156 neuroglobin Homo sapiens 93-104 27661977-3 2016 Experiments were conducted with phytoglobins from rice, tomato, and soybean along with human neuroglobin and soybean leghemoglobin that reveal hydroxylamine binding as the rate-limiting step. Hydroxylamine 143-156 leghemoglobin A Glycine max 117-130 27661977-4 2016 For hexacoordinate hemoglobins, binding is limited by the dissociation rate constant for the distal histidine, while leghemoglobin is limited by an intrinsically low affinity for hydroxylamine. Hydroxylamine 179-192 leghemoglobin A Glycine max 117-130 27661977-7 2016 Hexacoordinate hemoglobins such as neuroglobin are limited by tighter histidine coordination that blocks hydroxylamine binding, and pentacoordinate hemoglobins have intrinsically lower hydroxylamine affinities. Hydroxylamine 105-118 neuroglobin Homo sapiens 35-46 27702898-6 2016 Accelerating Schiff base hydrolysis and subsequent ATR dissociation, either by addition of hydroxylamine or introduction of mutations, further increased the time lag between ATR release and TM6 movement. Hydroxylamine 91-104 ATR serine/threonine kinase Homo sapiens 51-54 27702898-6 2016 Accelerating Schiff base hydrolysis and subsequent ATR dissociation, either by addition of hydroxylamine or introduction of mutations, further increased the time lag between ATR release and TM6 movement. Hydroxylamine 91-104 ATR serine/threonine kinase Homo sapiens 174-177 31380466-4 2016 The method for the assay of GS enzymatic activity relies on its gamma-glutamyl transferase reaction by measuring gamma-glutamylhydroxamate synthesized from glutamine and hydroxylamine, and the chromatographic separation of the reaction product from the reactants (Deuel et al., 1978). Hydroxylamine 170-183 glutamate-ammonia ligase Homo sapiens 28-30 27415751-1 2016 We have previously developed a novel class of bi-functional compounds based on a diarylidenyl-piperidone (DAP) backbone conjugated to an N-hydroxypyrroline (-NOH; a nitroxide precursor) group capable of selectively inhibiting STAT3 activation, translocation, and DNA binding activity. Hydroxylamine 165-174 signal transducer and activator of transcription 3 Homo sapiens 226-231 27320863-8 2016 Treatment with hydroxylamine, a cystathionine beta-synthase inhibitor, resulted in a reduction of pAMPK, pACC and pSREBP-1c, accompanied by an elevation of intracellular homocysteine. Hydroxylamine 15-28 cystathionine beta synthase Rattus norvegicus 32-59 27826125-0 2016 Nitroxides catalytically inhibit nitrite oxidation and heme inactivation induced by H2O2, nitrite and metmyoglobin or methemoglobin. Hydroxylamine 0-10 hemoglobin subunit gamma 2 Homo sapiens 118-131 33440533-2 2016 In this work, modified amorphous carbon films incorporating nitroxide groups (alpha-CNO) have been obtained by searching for a condensed analogue to classical soft antifouling materials. Hydroxylamine 60-69 biogenesis of lysosomal organelles complex 1 subunit 4 Homo sapiens 84-87 26893650-13 2016 At day 7, the apoptotic index and the expression of caspase-3 were reduced in the hippocampal CA1 region, while the expression of Bcl-2 increased in the NaHS group; and results of the hydroxylamine group were in contrast. Hydroxylamine 184-197 BCL2, apoptosis regulator Rattus norvegicus 130-135 27163749-6 2016 On the heme protein cytochrome C, we demonstrate that LaserIMD allows for distance measurements between a heme prosthetic group and a nitroxide label, although the heme triplet state is not directly observable by an electron spin echo. Hydroxylamine 134-143 cytochrome c, somatic Homo sapiens 20-32 26887942-2 2016 We have developed a novel docking strategy guided by paramagnetic NMR that positions a triple-helical collagen V mimic (synthesized with nitroxide spin labels) in the active site of the catalytic domain of matrix metalloproteinase-12 (MMP-12 or macrophage metalloelastase) primed for catalysis. Hydroxylamine 137-146 matrix metallopeptidase 12 Homo sapiens 206-233 26887942-2 2016 We have developed a novel docking strategy guided by paramagnetic NMR that positions a triple-helical collagen V mimic (synthesized with nitroxide spin labels) in the active site of the catalytic domain of matrix metalloproteinase-12 (MMP-12 or macrophage metalloelastase) primed for catalysis. Hydroxylamine 137-146 matrix metallopeptidase 12 Homo sapiens 235-241 26887942-2 2016 We have developed a novel docking strategy guided by paramagnetic NMR that positions a triple-helical collagen V mimic (synthesized with nitroxide spin labels) in the active site of the catalytic domain of matrix metalloproteinase-12 (MMP-12 or macrophage metalloelastase) primed for catalysis. Hydroxylamine 137-146 matrix metallopeptidase 12 Homo sapiens 245-271 26880412-9 2016 RESULTS: Hydroxylamine administration led to a significant decrease in erythropoietin, HIF-1alpha, HIF-2alpha and CBS protein levels during hypoxia. Hydroxylamine 9-22 erythropoietin Homo sapiens 71-85 26880412-9 2016 RESULTS: Hydroxylamine administration led to a significant decrease in erythropoietin, HIF-1alpha, HIF-2alpha and CBS protein levels during hypoxia. Hydroxylamine 9-22 cystathionine beta-synthase Homo sapiens 114-117 27000247-6 2016 In addition, NaHS-induced hyperalgesia was partly, but significantly, attenuated by intrathecal injection of hydroxylamine, a cystathionine-beta-synthase (CBS) inhibitor. Hydroxylamine 109-122 cystathionine beta-synthase Homo sapiens 126-153 26716570-6 2016 Reduction was found to comprise two consecutive reactions: a fast four-electron first-order reduction of the nitro-group to the hydroxylamine-intermediate (rate constant=0.28h(-1)) followed by a slower two-electron zero-order reduction resulting in the final amino product (rate constant=6.9muM h(-1)). Hydroxylamine 128-141 latexin Homo sapiens 291-294 26705481-3 2015 We used 1-hydroxy-3-carboxy-2,2,5,5-tetramethylpyrrolidine (CPH) as a reporter for one-electron oxidations, e.g. free radical-mediated oxidations; the one-electron oxidation product of CPH, 3-carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy (CP ), is a nitroxide free radical that is relatively persistent in vivo and detectable by EPR. Hydroxylamine 250-259 carboxypeptidase E Mus musculus 60-63 26518384-5 2016 Combining different strategies involving the grafting of nitroxide spin labels combined with Electron Paramagnetic Resonance (EPR) spectroscopy, the present study brings the first structural characterization, at the residue level, of yeast IF1 in its dimeric form. Hydroxylamine 57-66 ATP synthase inhibitory factor subunit 1 Bos taurus 240-243 26707812-11 2016 CCT also partially restored the decreased CBS expression and activity triggered by HG&MG, while the inhibition of CBS with hydroxylamine attenuated CCT-mediated neuroprotection. Hydroxylamine 127-140 cystathionine beta synthase Rattus norvegicus 118-121 28005032-7 2016 CSF lymphocytes were found to be ready for Fas-mediated apoptosis dependent on the receptor (CD95+ was 64.3%).There was a correlation using the pair correlation coefficient between the total concentration of the metabolites of the nitroxide molecule and the percentage of CD14+ (r=0.5; p<0.05). Hydroxylamine 231-240 Fas cell surface death receptor Homo sapiens 93-97 28005032-7 2016 CSF lymphocytes were found to be ready for Fas-mediated apoptosis dependent on the receptor (CD95+ was 64.3%).There was a correlation using the pair correlation coefficient between the total concentration of the metabolites of the nitroxide molecule and the percentage of CD14+ (r=0.5; p<0.05). Hydroxylamine 231-240 CD14 molecule Homo sapiens 272-276 26546694-2 2015 The aim of this study was to compare the efficiency of 11 nitroxides, derivatives of 2,2,6,6-tetramethylpiperidine-1-oxide (TEMPO) and 2,2,5,5-tetramethylpirrolidine-1-oxyl (PROXYL) in prevention of nitration and oxidation of model compounds and human serum albumin (HSA). Hydroxylamine 58-68 albumin Homo sapiens 252-265 26546694-9 2015 An exception was the prevention of thiol group oxidation by PN and SIN-1 where hydrophobic nitroxides were the most effective, apparently due to binding to the protein. Hydroxylamine 91-101 MAPK associated protein 1 Homo sapiens 67-72 26441482-3 2015 This ensures high oxygen concentration and oxygen diffusion at the location of the nitroxide as well as isolation of the nitroxide moiety from cellular reductants and paramagnetic ions that might interfere with spin-label oximetry measurements. Hydroxylamine 121-130 spindlin 1 Homo sapiens 211-215 26374996-9 2015 SOD2-tg mitochondria produced less superoxide, and had lower redox activity in converting cyclic hydroxylamine to stable nitroxide, and a lower GSSG concentration. Hydroxylamine 121-130 superoxide dismutase 2, mitochondrial Mus musculus 0-4 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 255-264 spindlin 1 Homo sapiens 20-24 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 255-264 spindlin 1 Homo sapiens 60-64 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 255-264 spindlin 1 Homo sapiens 60-64 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 375-384 spindlin 1 Homo sapiens 20-24 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 375-384 spindlin 1 Homo sapiens 60-64 26490692-1 2015 The large values of spin relaxation enhancement (RE) for PC spin-labels in the phospholipid membrane induced by paramagnetic metal salts dissolved in the aqueous phase can be explained by Heisenberg spin exchange due to conformational fluctuations of the nitroxide group as a result of membrane fluidity, flexibility of lipid chains, and, possibly, amphiphilic nature of the nitroxide label. Hydroxylamine 375-384 spindlin 1 Homo sapiens 60-64 26490692-2 2015 Whether the magnetic interaction occurs predominantly via Heisenberg spin exchange (Ni) or by the dipole-dipole (Gd) mechanism, it is essential for the paramagnetic ion to get into close proximity to the nitroxide moiety for efficient RE. Hydroxylamine 204-213 spindlin 1 Homo sapiens 69-73 26376730-3 2015 A beta-phosphorylated nitroxide substrate prototype exhibiting keto-enol equilibrium upon enzymatic activity has been prepared. Hydroxylamine 22-31 amyloid beta precursor protein Homo sapiens 0-6 26399494-4 2015 In this study, we developed a hydroxylamine assisted PNGase F deglycosylation (HAPD) method using the hydroxylamine to release glycopeptides captured on the hydrazide beads through the cleavage of hydrazone bonds by transamination followed with the PNGase F deglycosylation of the released glycopeptides in the free solution. Hydroxylamine 30-43 N-glycanase 1 Homo sapiens 53-59 26399494-4 2015 In this study, we developed a hydroxylamine assisted PNGase F deglycosylation (HAPD) method using the hydroxylamine to release glycopeptides captured on the hydrazide beads through the cleavage of hydrazone bonds by transamination followed with the PNGase F deglycosylation of the released glycopeptides in the free solution. Hydroxylamine 30-43 N-glycanase 1 Homo sapiens 249-255 26399494-4 2015 In this study, we developed a hydroxylamine assisted PNGase F deglycosylation (HAPD) method using the hydroxylamine to release glycopeptides captured on the hydrazide beads through the cleavage of hydrazone bonds by transamination followed with the PNGase F deglycosylation of the released glycopeptides in the free solution. Hydroxylamine 102-115 N-glycanase 1 Homo sapiens 53-59 26399494-4 2015 In this study, we developed a hydroxylamine assisted PNGase F deglycosylation (HAPD) method using the hydroxylamine to release glycopeptides captured on the hydrazide beads through the cleavage of hydrazone bonds by transamination followed with the PNGase F deglycosylation of the released glycopeptides in the free solution. Hydroxylamine 102-115 N-glycanase 1 Homo sapiens 249-255 26418890-5 2015 A 53-residue nitroxide EPR scan of the KCNE1 protein sequence including all 27 residues of the transmembrane domain (45-71) and 26 residues of the N- and C-termini of KCNE1 in lipid bilayered vesicles was analyzed in terms of nitroxide side-chain motion. Hydroxylamine 13-22 potassium voltage-gated channel subfamily E regulatory subunit 1 Homo sapiens 39-44 26418890-5 2015 A 53-residue nitroxide EPR scan of the KCNE1 protein sequence including all 27 residues of the transmembrane domain (45-71) and 26 residues of the N- and C-termini of KCNE1 in lipid bilayered vesicles was analyzed in terms of nitroxide side-chain motion. Hydroxylamine 226-235 potassium voltage-gated channel subfamily E regulatory subunit 1 Homo sapiens 39-44 26418890-6 2015 Continuous wave-EPR spectral line shape analysis indicated the nitroxide spin label side-chains located in the KCNE1 TMD are less mobile when compared to the extracellular region of KCNE1. Hydroxylamine 63-72 potassium voltage-gated channel subfamily E regulatory subunit 1 Homo sapiens 111-116 25569325-7 2015 The electrocatalytic activity of the Au/PPy electrodes was assessed for the electro-oxidation of hydrazine and hydroxylamine. Hydroxylamine 111-124 pancreatic polypeptide Homo sapiens 40-43 25986586-0 2015 Nitroxide delivery system for Nrf2 activation and skin protection. Hydroxylamine 0-9 NFE2 like bZIP transcription factor 2 Homo sapiens 30-34 25986586-4 2015 This approach would allow nitroxide activity and therefore their valuable effects (e.g. activation of the Keap1-Nrf2-EpRE pathway) to continue. Hydroxylamine 26-35 kelch like ECH associated protein 1 Homo sapiens 106-111 25986586-4 2015 This approach would allow nitroxide activity and therefore their valuable effects (e.g. activation of the Keap1-Nrf2-EpRE pathway) to continue. Hydroxylamine 26-35 NFE2 like bZIP transcription factor 2 Homo sapiens 112-116 25986586-7 2015 Our studies demonstrated that nitroxide microemulsions were more potent inducers of the Keap1-Nrf2-EpRE pathway than the free nitroxides, causing the activation of phase II enzymes. Hydroxylamine 30-39 kelch like ECH associated protein 1 Homo sapiens 88-93 25986586-7 2015 Our studies demonstrated that nitroxide microemulsions were more potent inducers of the Keap1-Nrf2-EpRE pathway than the free nitroxides, causing the activation of phase II enzymes. Hydroxylamine 30-39 NFE2 like bZIP transcription factor 2 Homo sapiens 94-98 26034980-3 2015 We found that tempol, a nitroxide, strongly induces the accumulation of hypoxia-inducible factor (HIF)-1alpha, particularly under conditions of hypoxia. Hydroxylamine 24-33 hypoxia inducible factor 1, alpha subunit Mus musculus 72-109 25979343-5 2015 To investigate this hypothesis we engineered a series of mutants along the length of the IKKgamma molecule that could be individually modified with nitroxide spin labels. Hydroxylamine 148-157 inhibitor of nuclear factor kappa B kinase regulatory subunit gamma Homo sapiens 89-97 25619120-6 2015 There were two reasons: (1) pH can influence the flow direction of electrons afforded by NH2OH oxidation; at high pH, electrons were mainly used for combining H+ and O2 (O2+4H++4e-=2H2O), the accumulation of NO2- cannot be a result of denitrification, and a higher DO can get more electrons to prefer NO2- and (2) NH4+ was the prerequisite for NH2OH oxidation, since NH2OH oxidation process was the way to provide electrons for nitrifier denitrification. Hydroxylamine 89-94 immunoglobulin kappa variable 1D-39 Homo sapiens 166-168 25619120-6 2015 There were two reasons: (1) pH can influence the flow direction of electrons afforded by NH2OH oxidation; at high pH, electrons were mainly used for combining H+ and O2 (O2+4H++4e-=2H2O), the accumulation of NO2- cannot be a result of denitrification, and a higher DO can get more electrons to prefer NO2- and (2) NH4+ was the prerequisite for NH2OH oxidation, since NH2OH oxidation process was the way to provide electrons for nitrifier denitrification. Hydroxylamine 89-94 immunoglobulin kappa variable 1D-39 Homo sapiens 170-172 25619120-6 2015 There were two reasons: (1) pH can influence the flow direction of electrons afforded by NH2OH oxidation; at high pH, electrons were mainly used for combining H+ and O2 (O2+4H++4e-=2H2O), the accumulation of NO2- cannot be a result of denitrification, and a higher DO can get more electrons to prefer NO2- and (2) NH4+ was the prerequisite for NH2OH oxidation, since NH2OH oxidation process was the way to provide electrons for nitrifier denitrification. Hydroxylamine 344-349 immunoglobulin kappa variable 1D-39 Homo sapiens 166-168 25619120-6 2015 There were two reasons: (1) pH can influence the flow direction of electrons afforded by NH2OH oxidation; at high pH, electrons were mainly used for combining H+ and O2 (O2+4H++4e-=2H2O), the accumulation of NO2- cannot be a result of denitrification, and a higher DO can get more electrons to prefer NO2- and (2) NH4+ was the prerequisite for NH2OH oxidation, since NH2OH oxidation process was the way to provide electrons for nitrifier denitrification. Hydroxylamine 344-349 immunoglobulin kappa variable 1D-39 Homo sapiens 170-172 25619120-6 2015 There were two reasons: (1) pH can influence the flow direction of electrons afforded by NH2OH oxidation; at high pH, electrons were mainly used for combining H+ and O2 (O2+4H++4e-=2H2O), the accumulation of NO2- cannot be a result of denitrification, and a higher DO can get more electrons to prefer NO2- and (2) NH4+ was the prerequisite for NH2OH oxidation, since NH2OH oxidation process was the way to provide electrons for nitrifier denitrification. Hydroxylamine 344-349 immunoglobulin kappa variable 1D-39 Homo sapiens 166-168 25619120-6 2015 There were two reasons: (1) pH can influence the flow direction of electrons afforded by NH2OH oxidation; at high pH, electrons were mainly used for combining H+ and O2 (O2+4H++4e-=2H2O), the accumulation of NO2- cannot be a result of denitrification, and a higher DO can get more electrons to prefer NO2- and (2) NH4+ was the prerequisite for NH2OH oxidation, since NH2OH oxidation process was the way to provide electrons for nitrifier denitrification. Hydroxylamine 344-349 immunoglobulin kappa variable 1D-39 Homo sapiens 170-172 25800440-3 2015 By monitoring spectral counts of specific hydroxamic acid signatures generated after the conversion of the ADP-ribose modification onto peptides by hydroxylamine hydrolysis, we undertook a thorough mass spectrometry mapping of the glutamate and aspartate ADP-ribosylation sites onto automodified PARP-1, PARP-2 and PARP-3. Hydroxylamine 148-161 poly(ADP-ribose) polymerase 1 Homo sapiens 296-302 25800440-3 2015 By monitoring spectral counts of specific hydroxamic acid signatures generated after the conversion of the ADP-ribose modification onto peptides by hydroxylamine hydrolysis, we undertook a thorough mass spectrometry mapping of the glutamate and aspartate ADP-ribosylation sites onto automodified PARP-1, PARP-2 and PARP-3. Hydroxylamine 148-161 poly(ADP-ribose) polymerase 2 Homo sapiens 304-310 25800440-3 2015 By monitoring spectral counts of specific hydroxamic acid signatures generated after the conversion of the ADP-ribose modification onto peptides by hydroxylamine hydrolysis, we undertook a thorough mass spectrometry mapping of the glutamate and aspartate ADP-ribosylation sites onto automodified PARP-1, PARP-2 and PARP-3. Hydroxylamine 148-161 poly(ADP-ribose) polymerase family member 3 Homo sapiens 315-321 25599573-6 2015 Microinjection of the CBS inhibitors hydroxylamine (HA) or amino-oxyacetate into the RVLM produced an increase in the renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP), and HR. Hydroxylamine 37-50 cystathionine beta synthase Rattus norvegicus 22-25 25599573-6 2015 Microinjection of the CBS inhibitors hydroxylamine (HA) or amino-oxyacetate into the RVLM produced an increase in the renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP), and HR. Hydroxylamine 52-54 cystathionine beta synthase Rattus norvegicus 22-25 25667417-5 2015 Catalase was detected in primary HSC and a stromal cell line, and addition of the competitive catalase inhibitor hydroxylamine resulted in a dose-dependent impairment of MDSC induction and concomitant increase of H2O2 levels. Hydroxylamine 113-126 catalase Homo sapiens 0-8 25667417-5 2015 Catalase was detected in primary HSC and a stromal cell line, and addition of the competitive catalase inhibitor hydroxylamine resulted in a dose-dependent impairment of MDSC induction and concomitant increase of H2O2 levels. Hydroxylamine 113-126 catalase Homo sapiens 94-102 25894215-1 2015 An electrochemical sandwich immunoassay method that can be sensitive to a few protein molecules (human immunoglobulin G or human prostate-specific antigen) is reported, based on HAuCl4-NH2OH redox reaction to enlarge the size of second antibody labeled gold nanoparticles and in situ microliter-droplet anodic stripping voltammetry analysis with enhanced cathodic preconcentration of gold. Hydroxylamine 185-190 kallikrein related peptidase 3 Homo sapiens 129-154 25630260-4 2015 Conversely, other CSE antagonists tested, aminooxyacetic acid (AOAA, 100 mum), beta-cyanoalanine (BCA, 500 mum) and hydroxylamine (HA, 100 mum), altered the NPV to PGF2alpha (BCA increased, HA inhibited) and/or LY83583 (BCA increased, AOAA and HA inhibited). Hydroxylamine 116-129 cystathionine gamma-lyase Rattus norvegicus 18-21 25301946-7 2014 Treatment with 1 m hydroxylamine for 1 h removes the fatty acids from a majority of the SLN pool. Hydroxylamine 19-32 sarcolipin Oryctolagus cuniculus 88-91 25677087-5 2015 In this work we have designed and studied two new pyrrolidine mitochondria targeted nitroxides: 3-[2-(triphenyphosphonio)acetamido]- and 3-[2-(triphenyphosphonio) acetamidomethyl]-2,2,5,5-tetramethylpyrrolidine-1-oxyl (mCP2) and (mCP1). Hydroxylamine 84-94 cleft palate 2 Mus musculus 219-223 25677087-5 2015 In this work we have designed and studied two new pyrrolidine mitochondria targeted nitroxides: 3-[2-(triphenyphosphonio)acetamido]- and 3-[2-(triphenyphosphonio) acetamidomethyl]-2,2,5,5-tetramethylpyrrolidine-1-oxyl (mCP2) and (mCP1). Hydroxylamine 84-94 cleft palate 2 Mus musculus 230-234 25677087-6 2015 These new mitochondria targeted nitroxides have 3- to 7-fold lower rate constants of the reaction with O2(- ) compared with mitoTEMPO; however, the cellular bioreduction of mCP1 and mCP2 was 3- and 2-fold slower. Hydroxylamine 32-42 cleft palate 2 Mus musculus 173-177 25677087-6 2015 These new mitochondria targeted nitroxides have 3- to 7-fold lower rate constants of the reaction with O2(- ) compared with mitoTEMPO; however, the cellular bioreduction of mCP1 and mCP2 was 3- and 2-fold slower. Hydroxylamine 32-42 cleft palate 2 Mus musculus 182-186 25677087-7 2015 As a consequence incubation with cells afforded much higher intracellular concentration of mCP1 and mCP2 nitroxides compared to mitoTEMPO nitroxide. Hydroxylamine 105-115 cleft palate 2 Mus musculus 100-104 25677087-12 2015 Studies of nitroxide analogs such as mCP1 and mCP2 may help in optimization of chemical structure of mitochondria-targeted nitroxides for improved efficacy and pharmacokinetics of these drugs in treatment of hypertension and many other conditions including atherosclerosis, diabetes and degenerative neurological disorders in which mitochondrial oxidative stress seems to play a role. Hydroxylamine 11-20 cleft palate 2 Mus musculus 37-41 25677087-12 2015 Studies of nitroxide analogs such as mCP1 and mCP2 may help in optimization of chemical structure of mitochondria-targeted nitroxides for improved efficacy and pharmacokinetics of these drugs in treatment of hypertension and many other conditions including atherosclerosis, diabetes and degenerative neurological disorders in which mitochondrial oxidative stress seems to play a role. Hydroxylamine 11-20 cleft palate 2 Mus musculus 46-50 25677087-12 2015 Studies of nitroxide analogs such as mCP1 and mCP2 may help in optimization of chemical structure of mitochondria-targeted nitroxides for improved efficacy and pharmacokinetics of these drugs in treatment of hypertension and many other conditions including atherosclerosis, diabetes and degenerative neurological disorders in which mitochondrial oxidative stress seems to play a role. Hydroxylamine 123-133 cleft palate 2 Mus musculus 37-41 25677087-12 2015 Studies of nitroxide analogs such as mCP1 and mCP2 may help in optimization of chemical structure of mitochondria-targeted nitroxides for improved efficacy and pharmacokinetics of these drugs in treatment of hypertension and many other conditions including atherosclerosis, diabetes and degenerative neurological disorders in which mitochondrial oxidative stress seems to play a role. Hydroxylamine 123-133 cleft palate 2 Mus musculus 46-50 25374015-4 2015 The activator, S-adenosyl-L-methionine and the inhibitor, hydroxylamine of cystathionine-beta-synthase (CBS), aggravated and remitted the hypoxic damage in the cortex cells, respectively. Hydroxylamine 58-71 cystathionine beta synthase Rattus norvegicus 75-102 25994134-2 2015 Formyl-Cbl, acetyl-Cbl, and propionyl-Cbl were decomposed by a NH2OH treatment, forming formo-, aceto-, and propionohydroxamic acids, respectively, which offers a proof for the presence of "activated" acyl groups and for their structures of Co-acyl-Cbls. Hydroxylamine 63-68 Cbl proto-oncogene Homo sapiens 7-10 25994134-2 2015 Formyl-Cbl, acetyl-Cbl, and propionyl-Cbl were decomposed by a NH2OH treatment, forming formo-, aceto-, and propionohydroxamic acids, respectively, which offers a proof for the presence of "activated" acyl groups and for their structures of Co-acyl-Cbls. Hydroxylamine 63-68 Cbl proto-oncogene Homo sapiens 19-22 25994134-2 2015 Formyl-Cbl, acetyl-Cbl, and propionyl-Cbl were decomposed by a NH2OH treatment, forming formo-, aceto-, and propionohydroxamic acids, respectively, which offers a proof for the presence of "activated" acyl groups and for their structures of Co-acyl-Cbls. Hydroxylamine 63-68 Cbl proto-oncogene Homo sapiens 19-22 26477246-0 2015 Saturation Recovery EPR and Nitroxide Spin Labeling for Exploring Structure and Dynamics in Proteins. Hydroxylamine 28-37 spindlin 1 Homo sapiens 38-42 25299573-3 2014 Among successful alternatives to native chemical ligation, are the alpha-ketoacid-hydroxylamine ligation with 5-oxaproline and, serine/threonine ligation, and potassium acyltrifluoroborate (KAT) ligation. Hydroxylamine 82-95 thiosulfate sulfurtransferase like domain containing 1 Homo sapiens 190-193 25247738-3 2014 The ability of these relatively short mimetics to bind to CR3 and dectin-1, as compared to the greater degree of polymerization required in beta-(1 3)-glucans, is discussed in terms of the increased hydrophobicity of the alpha-face on replacement of the glycosidic bond by the hydroxylamine linkage. Hydroxylamine 277-290 integrin alpha M Mus musculus 58-61 25236737-0 2014 Can nitroxides evoke the Keap1-Nrf2-ARE pathway in skin? Hydroxylamine 4-14 kelch like ECH associated protein 1 Homo sapiens 25-30 25236737-0 2014 Can nitroxides evoke the Keap1-Nrf2-ARE pathway in skin? Hydroxylamine 4-14 NFE2 like bZIP transcription factor 2 Homo sapiens 31-35 25236737-6 2014 This study shows that nitroxides may act as electrophiles, directly or indirectly, capable of activating the Keap1-Nrf2-ARE pathway in human keratinocytes (HaCaT) and in human skin (human organ culture model). Hydroxylamine 22-32 kelch like ECH associated protein 1 Homo sapiens 109-114 25236737-6 2014 This study shows that nitroxides may act as electrophiles, directly or indirectly, capable of activating the Keap1-Nrf2-ARE pathway in human keratinocytes (HaCaT) and in human skin (human organ culture model). Hydroxylamine 22-32 NFE2 like bZIP transcription factor 2 Homo sapiens 115-119 25236737-8 2014 The mechanism of action by which nitroxides activate the Keap1-Nrf2-ARE pathway is discussed. Hydroxylamine 33-43 kelch like ECH associated protein 1 Homo sapiens 57-62 25236737-8 2014 The mechanism of action by which nitroxides activate the Keap1-Nrf2-ARE pathway is discussed. Hydroxylamine 33-43 NFE2 like bZIP transcription factor 2 Homo sapiens 63-67 25382045-12 2014 After Cr(VI) has been reduced to Cr(III) with hydroxylamine hydrochloride, the total amount of chromium was obtained, and the content of Cr(VI) was given by subtraction. Hydroxylamine 46-73 cyclin 3 Zea mays 33-40 24729587-3 2014 In this paper, this concept is illustrated in vivo at 0.2 T using nitroxide-labeled elastin orally administered in mice. Hydroxylamine 66-75 elastin Mus musculus 84-91 24729587-4 2014 In vitro, this elastin derivative was OMRI-visible and gave rise to high Overhauser enhancements (19-fold at 18 mm nitroxide) upon proteolysis by pancreatic porcine elastase. Hydroxylamine 115-124 elastin Mus musculus 15-22 24964273-0 2014 Can hydroxylamine be a more potent nucleophile for the reactivation of tabun-inhibited AChE than prototype oxime drugs? Hydroxylamine 4-17 acetylcholinesterase (Cartwright blood group) Homo sapiens 87-91 24603843-3 2014 Here, we provide a solution to this issue and present an approach to design high energy and high power battery electrodes by hybridizing a nitroxide-polymer redox supercapacitor (PTMA) with a Li-ion battery material (LiFePO4). Hydroxylamine 139-148 prothymosin alpha Homo sapiens 179-183 24434189-1 2014 Aldo-keto reductase 1C3 (AKR1C3, EC 1.1.1.188) metabolises steroid hormones, prostaglandins and xenobiotics, and activates the dinitrobenzamide mustard prodrug PR-104A by reducing it to hydroxylamine PR-104H. Hydroxylamine 186-199 aldo-keto reductase family 1 member C3 Homo sapiens 0-23 24434189-1 2014 Aldo-keto reductase 1C3 (AKR1C3, EC 1.1.1.188) metabolises steroid hormones, prostaglandins and xenobiotics, and activates the dinitrobenzamide mustard prodrug PR-104A by reducing it to hydroxylamine PR-104H. Hydroxylamine 186-199 aldo-keto reductase family 1 member C3 Homo sapiens 25-31 24462868-0 2014 Hydroxylamine enhances glucose uptake in C2C12 skeletal muscle cells through the activation of insulin receptor substrate 1. Hydroxylamine 0-13 insulin receptor substrate 1 Mus musculus 95-123 24293651-5 2014 Multiple nanometer distances in the p21-RE and BAX-RE, measured using a nucleotide-independent nitroxide probe and double-electron-electron-resonance spectroscopy, were used to derive molecular models of unbound REs from pools of all-atom structures generated by Monte-Carlo simulations, thus enabling analyses to reveal sequence-dependent DNA shape features of unbound REs in solution. Hydroxylamine 95-104 H3 histone pseudogene 16 Homo sapiens 36-39 24293651-5 2014 Multiple nanometer distances in the p21-RE and BAX-RE, measured using a nucleotide-independent nitroxide probe and double-electron-electron-resonance spectroscopy, were used to derive molecular models of unbound REs from pools of all-atom structures generated by Monte-Carlo simulations, thus enabling analyses to reveal sequence-dependent DNA shape features of unbound REs in solution. Hydroxylamine 95-104 BCL2 associated X, apoptosis regulator Homo sapiens 47-50 24862953-2 2014 AOB use (i) ammonia monooxygenase for biological ammonia (NH3) oxidation to hydroxylamine (NH2OH) and (ii) hydroxylamine oxidoreductase for NH2OH oxidation to nitrite. Hydroxylamine 140-145 thioredoxin reductase 1 Homo sapiens 121-135 24971930-6 2014 The effect of d-serine on ERK activation was confirmed by administering d-serine dehydratase, an enzyme that specifically degrades d-serine, and the enzyme"s inhibitor, hydroxylamine. Hydroxylamine 169-182 extracellular regulated MAP kinase Bombyx mori 26-29 24936972-3 2014 The labile alkoxyamine ALK-1 (t(1/2) = 50 min at 37 C) cleaves spontaneously to generate (1) a highly reactive free alkyl radical used as therapeutic agents to induce cell damages leading to cell death and (2) a stable nitroxide used as contrast agent for Overhauser-enhanced magnetic resonance imaging (OMRI). Hydroxylamine 220-229 secretory leukocyte peptidase inhibitor Homo sapiens 23-28 24936972-9 2014 The nitroxide production, during the alkoxyamine homolysis, was monitored by OMRI, showing a progressive MRI signal enhancement to 6-fold concomitant to the ALK-1 homolysis. Hydroxylamine 4-13 secretory leukocyte peptidase inhibitor Homo sapiens 157-162 24566469-6 2014 421, 79-86, 2009) that nitroxides, including 4-amino-TEMPO (4-amino-2,2,6,6-tetramethylpiperidin-1-yloxyl radical), are potent inhibitors of HOCl formation by isolated MPO and activated neutrophils, with IC50 values of ~1 and ~6 microM respectively. Hydroxylamine 23-33 myeloperoxidase Homo sapiens 55-58 24452480-0 2014 Diversification of EPR signatures in Site Directed Spin Labeling using a beta-phosphorylated nitroxide. Hydroxylamine 93-102 spindlin 1 Homo sapiens 51-55 24452480-2 2014 Conventional spin labels are based on nitroxide derivatives leading to classical 3-line spectra whose spectral shapes are indicative of the environment of the labels and thus constitute good reporters of structural modifications. Hydroxylamine 38-47 spindlin 1 Homo sapiens 13-17 24452480-4 2014 To overcome the limitation due to the weak diversity of nitroxide label EPR spectral shapes, we designed a new spin label based on a beta-phosphorylated nitroxide giving 6-line spectra. Hydroxylamine 56-65 spindlin 1 Homo sapiens 111-115 24452480-4 2014 To overcome the limitation due to the weak diversity of nitroxide label EPR spectral shapes, we designed a new spin label based on a beta-phosphorylated nitroxide giving 6-line spectra. Hydroxylamine 153-162 spindlin 1 Homo sapiens 111-115 24337356-4 2014 Alkoxyamines (R(1)R(2)NOR(3)) are labile molecules that homolyze into nitroxides (R(1)R(2)NO ) and reactive alkyl radicals (R(3) ). Hydroxylamine 70-80 CD1d molecule Homo sapiens 24-28 24271207-0 2014 Heme-bound nitroxyl, hydroxylamine, and ammonia ligands as intermediates in the reaction cycle of cytochrome c nitrite reductase: a theoretical study. Hydroxylamine 21-34 cytochrome c, somatic Homo sapiens 98-110 24144032-2 2013 METHODS: Superoxide dismutase (SOD) activity was measured by hydroxylamine colorimetric method. Hydroxylamine 61-74 superoxide dismutase 1 Homo sapiens 9-29 24144032-2 2013 METHODS: Superoxide dismutase (SOD) activity was measured by hydroxylamine colorimetric method. Hydroxylamine 61-74 superoxide dismutase 1 Homo sapiens 31-34 24056696-6 2013 Hydroxylamine, a potent nucleophilic cellular metabolite, may have therapeutic potential for INCL, but its toxicity precludes clinical application. Hydroxylamine 0-13 palmitoyl-protein thioesterase 1 Mus musculus 93-97 24056696-7 2013 We found that a hydroxylamine derivative, N-(tert-Butyl) hydroxylamine (NtBuHA), was non-toxic, cleaved thioester linkage in palmitoylated proteins and mediated lysosomal ceroid depletion in cultured cells from INCL patients. Hydroxylamine 16-29 palmitoyl-protein thioesterase 1 Homo sapiens 211-215 24380243-1 2013 Irwin B. Wilson, working in the laboratory of David Nachmansohn at Columbia, demonstrated the ability of hydroxylamine to reactivate cholinesterase inhibited by organophosphates. Hydroxylamine 105-118 butyrylcholinesterase Homo sapiens 133-147 24954957-1 2013 The electron paramagnetic resonance technique of double electron-electron resonance (DEER) was used to measure nanometre-scale distances between nitroxide spin labels attached to the complement regulatory protein CD55 (also known as decay accelerating factor) and the von Willebrand factor A (vWF-A) domain of factor B. Hydroxylamine 145-154 CD55 molecule (Cromer blood group) Homo sapiens 213-217 23796515-5 2013 Using the environmentally sensitive fluorescent probe attached to the cytoplasmic domain of CLS and the nitroxide quencher attached to the lipid bilayer, we showed that the association of moesin FERM domain induced the desorption of the basic-rich cytoplasmic domain of CLS from the anionic membrane surface, which enabled subsequent association of CaM to the cytoplasmic domain of CLS. Hydroxylamine 104-113 moesin Homo sapiens 188-194 23881822-7 2013 To achieve this goal, 15-residue segments of TCRalpha, containing the CP, were synthesized and spin-labeled at different locations with a nitroxide derivative. Hydroxylamine 138-147 T cell receptor alpha constant Homo sapiens 45-53 23480813-4 2013 Reduction of FSL-61 by purified recombinant human POR generated the corresponding hydroxylamine, which was non-fluorescent, but was reduced further to the fluorescent amine in cells. Hydroxylamine 82-95 cytochrome p450 oxidoreductase Homo sapiens 50-53 23728586-8 2013 This effect could be diminished by the ATP-sensitive potassium ion (KATP) channel blocker glibenclamide (GLB), the CSE inhibitor DL-propargylglycine (PPG) and the CBS inhibitor hydroxylamine (HA). Hydroxylamine 177-190 cystathionine beta-synthase Homo sapiens 163-166 26097251-2 2013 In an attempt to observe dynamic nuclear polarization (DNP) in an MRFM experiment, which could possibly further improve its sensitivity towards a single proton spin, a film of perdeuterated polystyrene doped with a nitroxide electron-spin probe was prepared. Hydroxylamine 215-224 spindlin 1 Homo sapiens 160-164 26097251-2 2013 In an attempt to observe dynamic nuclear polarization (DNP) in an MRFM experiment, which could possibly further improve its sensitivity towards a single proton spin, a film of perdeuterated polystyrene doped with a nitroxide electron-spin probe was prepared. Hydroxylamine 215-224 spindlin 1 Homo sapiens 234-238 26097251-4 2013 In addition to observing the expected prompt change in cantilever frequency due to saturation of the nitroxide"s electron-spin magnetization, we observed a persistent cantilever frequency change. Hydroxylamine 101-110 spindlin 1 Homo sapiens 122-126 23642211-5 2013 This nitroxide has been synthesized and successfully grafted successively on p-cresol, a small tetrapeptide and a model protein: a small chloroplastic protein CP12 having functional cysteines and a single tyrosine. Hydroxylamine 5-14 cytochrome P450 family 1 subfamily A member 2 Homo sapiens 159-163 23488457-10 2013 Trifluoroalanine and hydroxylamine, two compounds that have also been used to block H2S biosynthesis, blocked the activity of CBS and CSE. Hydroxylamine 21-34 cystathionine beta-synthase Homo sapiens 126-129 23906368-4 2013 The DEER distance between nitroxide spin-labels attached at C39 and N120C is 2.5 +- 0.1 nm for Ca2+-free recoverin and 3.7 +- 0.1 nm for Ca2+-bound recoverin. Hydroxylamine 26-35 recoverin Homo sapiens 105-114 23906368-4 2013 The DEER distance between nitroxide spin-labels attached at C39 and N120C is 2.5 +- 0.1 nm for Ca2+-free recoverin and 3.7 +- 0.1 nm for Ca2+-bound recoverin. Hydroxylamine 26-35 recoverin Homo sapiens 148-157 23819749-2 2013 Insulin was successfully spin-labeled with iodoacetamide and the bifunctional nitroxide reagent HO-1944. Hydroxylamine 78-87 insulin Homo sapiens 0-7 23731861-0 2013 Conformational dynamics and distribution of nitroxide spin labels. Hydroxylamine 44-53 spindlin 1 Homo sapiens 54-58 23532887-5 2013 RESULTS: In the early stage of cancer, the brain tissues were characterized by a shorter-lived MRI signal than that from healthy brains (indicating a higher reducing activity for the nitroxide radical), which was accompanied by an enhancement of TTAC and MMP9 plasma levels. Hydroxylamine 183-192 matrix metallopeptidase 9 Mus musculus 255-259 23507756-2 2013 The rapid and room temperature synthesis of magnetic nanoparticles was achieved using the hydroxylamine reduction of HAuCl4 on the surface of ethylenediaminetetraacetic acid (EDTA)-immobilized iron (magnetite Fe3O4) nanoparticles in the presence of an aqueous solution of hexadecyltrimetylammonium bromide (CTAB) as a dispersant. Hydroxylamine 90-103 outer membrane receptor FepA Escherichia coli 193-197 23404373-9 2013 Our findings support the mechanism of lapatinib CYP3A4 inactivation as MI complex formation with the nitroso metabolite formed through the secondary hydroxylamine and nitrone pathway, rather than by N-dealkylation to the primary amine followed by N-hydroxylation and dehydrogenation as is usually assumed. Hydroxylamine 149-162 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 48-54 23944056-12 2013 The activity of choline-acetyl-transfertase(ChAT) and acetylcholinesterase (AChE) in hippocampus and cortex of rats were measured by radiochemical method and hydroxylamine colorimetry separately. Hydroxylamine 158-171 choline O-acetyltransferase Rattus norvegicus 44-48 23556735-0 2013 1H relaxation dispersion in solutions of nitroxide radicals: influence of electron spin relaxation. Hydroxylamine 41-50 spindlin 1 Homo sapiens 83-87 23402364-6 2013 Separation and spectral analysis of VP-16 reaction extracts by electron spin resonance and UV-vis indicated the generation of the phenoxy radical and the o-quinone of VP-16, as well as putative nitroxide, iminoxyl, and other nitrogen oxide intermediates. Hydroxylamine 194-203 host cell factor C1 Homo sapiens 36-41 23320703-1 2013 For nitroxide radicals in solution one can identify three frequency regimes in which (1)H spin-lattice relaxation rate of solvent molecules depend linearly on square root of the (1)H resonance frequency. Hydroxylamine 4-13 spindlin 1 Homo sapiens 90-94 23469112-4 2013 METHODOLOGY/PRINCIPAL FINDINGS: We designed an elastase substrate by grafting stable nitroxide free radicals on soluble elastin. Hydroxylamine 85-94 elastin Homo sapiens 120-127 23273102-4 2013 The CBS inhibitors hydroxylamine and aminooxyacetic acid attenuated mechanical hyperalgesia in a dose-dependent manner and reversed hyperexcitability of DRG neurons in inflamed rats. Hydroxylamine 19-32 cystathionine beta synthase Rattus norvegicus 4-7 22824865-0 2012 Ceruloplasmin (ferroxidase) oxidizes hydroxylamine probes: deceptive implications for free radical detection. Hydroxylamine 37-50 ceruloplasmin Homo sapiens 0-13 22982597-0 2012 Nitroxides attenuate carrageenan-induced inflammation in rat paws by reducing neutrophil infiltration and the resulting myeloperoxidase-mediated damage. Hydroxylamine 0-10 myeloperoxidase Rattus norvegicus 120-135 22982597-2 2012 To examine whether nitroxides inhibit MPO activity in vivo we selected acute carrageenan-induced inflammation on the rat paw as a model. Hydroxylamine 19-29 myeloperoxidase Rattus norvegicus 38-41 22982597-4 2012 All of the tested nitroxides inhibited the chlorinating activity of MPO in vitro with similar IC(50) values (between 1.5 and 1.8 muM). Hydroxylamine 18-28 myeloperoxidase Rattus norvegicus 68-71 22982597-7 2012 Likewise, paw edema, levels of nitrated and oxidized proteins, and levels of plasma exudation correlated with the levels of MPO protein in the paws of the animals that were untreated or treated with the nitroxides. Hydroxylamine 203-213 myeloperoxidase Rattus norvegicus 124-127 22982597-9 2012 Taken together, the results indicate that nitroxides attenuate carrageenan-induced inflammation mainly by reducing neutrophil migration and the resulting MPO-mediated damage. Hydroxylamine 42-52 myeloperoxidase Rattus norvegicus 154-157 26605629-4 2012 The results from molecular dynamics simulations suggest that the oligothiophene conjugate part of the spin label interacts with hydrophobic residues of the amyloid protein through hydrophobic attraction and that both the N-O bond length and the N-O out-of-plane tilt angle in the nitroxide group are slightly diminished after complexation with the protein. Hydroxylamine 280-289 spindlin 1 Homo sapiens 102-106 26605629-5 2012 The translational and rotational motions of the protein-bound spin label are considerably slowed compared to those of the free spin label in aqueous solution, but interestingly, hydrogen bonds formed between the nitroxide oxygen group and the surrounding water molecules are hardly affected by the presence of the amyloid protein. Hydroxylamine 212-221 spindlin 1 Homo sapiens 62-66 26605629-6 2012 First-principles calculations suggest that EPR spin Hamiltonian parameters including the nitroxide nitrogen hyperfine coupling tensor A(N) and electronic g tensor suffer noticeable changes upon complexation with the protein. Hydroxylamine 89-98 spindlin 1 Homo sapiens 47-51 22917445-0 2012 Synthesis of 2,5-bis(spirocyclohexane)-substituted nitroxides of pyrroline and pyrrolidine series, including thiol-specific spin label: an analogue of MTSSL with long relaxation time. Hydroxylamine 51-61 spindlin 1 Homo sapiens 124-128 23136724-0 2012 The history of cholinesterase reactivation: hydroxylamine and pyridinium aldoximes. Hydroxylamine 44-57 butyrylcholinesterase Homo sapiens 15-29 23136724-1 2012 Hydroxylamine (NH2OH) the substance which will turn out to be of importance to those interested in the treatment of organophosporus cholinesterase inhibitor exposure, was synthesized by Wilhem Clemens Lossen in 1865 while working in Halle as an assistant in the laboratory of Wilhelm Heinrich Heintz. Hydroxylamine 0-13 butyrylcholinesterase Homo sapiens 132-146 23136724-1 2012 Hydroxylamine (NH2OH) the substance which will turn out to be of importance to those interested in the treatment of organophosporus cholinesterase inhibitor exposure, was synthesized by Wilhem Clemens Lossen in 1865 while working in Halle as an assistant in the laboratory of Wilhelm Heinrich Heintz. Hydroxylamine 15-20 butyrylcholinesterase Homo sapiens 132-146 23136724-6 2012 Some fifty years later Wilson, working in the laboratory of Nachmansohn, demonstrated the ability of hydroxylamine to reactivate cholinesterase inhibited by organophosphates. Hydroxylamine 101-114 butyrylcholinesterase Homo sapiens 129-143 22917445-1 2012 The nitroxides of 7-azadispiro[5.1.5.2]pentadecane and 7-azadispiro[5.1.5.2]pentadeca-14-ene series have been prepared, including thiol-specific methane thiosulfonate spin label for site-directed spin labeling. Hydroxylamine 4-14 spindlin 1 Homo sapiens 167-171 22917445-1 2012 The nitroxides of 7-azadispiro[5.1.5.2]pentadecane and 7-azadispiro[5.1.5.2]pentadeca-14-ene series have been prepared, including thiol-specific methane thiosulfonate spin label for site-directed spin labeling. Hydroxylamine 4-14 spindlin 1 Homo sapiens 196-200 22917445-3 2012 The obtained temperature dependencies of electron spin relaxation parameters demonstrate that new nitroxides may be suitable for PELDOR distance measurements at 80-120 K. Moreover, the new nitroxides demonstrated much higher stability toward reduction by ascorbate than spirocyclohexane-substituted nitroxides of piperidine series and showed 1.3-3.14 times lower reduction rates compared to corresponding 2,2,5,5-tetramethyl nitroxides. Hydroxylamine 98-108 spindlin 1 Homo sapiens 50-54 22917445-3 2012 The obtained temperature dependencies of electron spin relaxation parameters demonstrate that new nitroxides may be suitable for PELDOR distance measurements at 80-120 K. Moreover, the new nitroxides demonstrated much higher stability toward reduction by ascorbate than spirocyclohexane-substituted nitroxides of piperidine series and showed 1.3-3.14 times lower reduction rates compared to corresponding 2,2,5,5-tetramethyl nitroxides. Hydroxylamine 189-199 spindlin 1 Homo sapiens 50-54 22816446-1 2012 BACKGROUND: Delayed hypersensitivity (HS) reactions to potentiated sulfonamide antimicrobials occur in both dogs and humans, and involve an intermediate hydroxylamine metabolite that is detoxified by cytochrome b(5) and NADH cytochrome b(5) reductase. Hydroxylamine 153-166 cytochrome b5 type A Homo sapiens 200-215 22816446-1 2012 BACKGROUND: Delayed hypersensitivity (HS) reactions to potentiated sulfonamide antimicrobials occur in both dogs and humans, and involve an intermediate hydroxylamine metabolite that is detoxified by cytochrome b(5) and NADH cytochrome b(5) reductase. Hydroxylamine 153-166 cytochrome b5 type A Homo sapiens 225-240 22804493-4 2012 For H(2)O@K-8, 17 positional isomeric nitroxides are predicted, not including additional numbers of regioisomers; indeed, 17 signals are observed in the (1)H NMR spectrum. Hydroxylamine 38-48 keratin 8 Homo sapiens 10-13 23157194-1 2012 Sulfonamide antimicrobials (sulfamethoxazole) contain an arylamine group, oxidized by CYP2C9 to the hydroxylamine with subsequent auto-oxidation to a highly reactive [-nitroso-] intermediate is a necessary (if not sufficient) cause of drug hypersensitivity. Hydroxylamine 100-113 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 86-92 22874131-9 2012 Our results show that with respect to hydroxylamine sensitivity and retinal release, the wild-type echidna rhodopsin displays major differences to all previously characterized mammalian rhodopsins and appears more similar to other nonmammalian vertebrate rhodopsins such as chicken and anole. Hydroxylamine 38-51 rhodopsin Homo sapiens 107-116 22549882-6 2012 UV-visible absorbance spectroscopy revealed hydroxylamine accessibility to the chromophore-binding pocket of dark state G90D rhodopsin, which is not detected in dark state wild-type rhodopsin but is detected in light-activated wild-type rhodopsin. Hydroxylamine 44-57 rhodopsin Mus musculus 125-134 22549882-6 2012 UV-visible absorbance spectroscopy revealed hydroxylamine accessibility to the chromophore-binding pocket of dark state G90D rhodopsin, which is not detected in dark state wild-type rhodopsin but is detected in light-activated wild-type rhodopsin. Hydroxylamine 44-57 rhodopsin Mus musculus 182-191 22549882-6 2012 UV-visible absorbance spectroscopy revealed hydroxylamine accessibility to the chromophore-binding pocket of dark state G90D rhodopsin, which is not detected in dark state wild-type rhodopsin but is detected in light-activated wild-type rhodopsin. Hydroxylamine 44-57 rhodopsin Mus musculus 182-191 22547062-10 2012 Melanopsin was highly resistant to both visible-spectrum photic bleaching and chemical bleaching with hydroxylamine under conditions that fully bleach rod and cone photoreceptor cells. Hydroxylamine 102-115 opsin 4 Homo sapiens 0-10 22483878-5 2012 The modified electrode was applied to detection hydroxylamine in the tap water, and the average recovery for the standards added was 96.0%. Hydroxylamine 48-61 nuclear RNA export factor 1 Homo sapiens 69-72 22121042-2 2012 Pentafluorophenyl 4-maleimidobenzoate is kinetically installed at different stages of a nitroxide-mediated polymerization, namely, near the alpha-chain end and in the middle of a PS chain. Hydroxylamine 88-97 Fc gamma receptor and transporter Homo sapiens 140-151 22510038-7 2012 Hydroxylamine cleaves the thioester adduct; substantial enzyme activity is restored at a rate that is 8-fold faster for human HMGCS than for mvaS. Hydroxylamine 0-13 3-hydroxy-3-methylglutaryl-CoA synthase 1 Homo sapiens 126-131 21850569-2 2012 We have demonstrated the process of reactivating the VX-AChE adduct with formoximate and hydroxylamine anions by applying the DFT approach at the B3LYP/6-311 G(d,p) level of theory. Hydroxylamine 89-102 acetylcholinesterase (Cartwright blood group) Homo sapiens 56-60 22266510-2 2012 METHODS: Rhodopsin photolysis was studied using UV-visible spectroscopy and rapid scanning spectroscopy in the presence of hydroxylamine in highly purified wild-type and Gtgamma-deficient mouse rod disc membranes. Hydroxylamine 123-136 rhodopsin Mus musculus 9-18 26592886-1 2012 Encapsulation of a nitroxide spin label into a host cavity can prolong the lifetime of the spin label in biological tissues and other environments. Hydroxylamine 19-28 spindlin 1 Homo sapiens 29-33 26592886-1 2012 Encapsulation of a nitroxide spin label into a host cavity can prolong the lifetime of the spin label in biological tissues and other environments. Hydroxylamine 19-28 spindlin 1 Homo sapiens 91-95 21896789-6 2011 Treatment of the glucuronide metabolites 3 and 4 with beta-glucuronidase gave mainly the expected hydrolysis product, the hydroxyl amine 4-[2-(2,4-dimethyl-phenylsulfanyl)-phenyl]-piperazin-1-ol (2). Hydroxylamine 122-136 glucuronidase beta Homo sapiens 54-72 22081607-5 2012 We identified ~300 17-octadecynoic acid-modified and hydroxylamine-sensitive proteins, of which a subset was decreased in abundance in DHHC5-GT cells. Hydroxylamine 53-66 zinc finger, DHHC domain containing 5 Mus musculus 135-140 21737567-2 2011 Encapsulating nitroxides in anti-HER2 immunoliposomes at high concentrations to take advantage of the "self-quenching" phenomenon of nitroxides allows generation of robust EPR signals in HER2-overexpressing breast tumor cells with minimal background from indifferent tissues or circulating liposomes. Hydroxylamine 133-143 erb-b2 receptor tyrosine kinase 2 Homo sapiens 33-37 21749327-9 2011 After turnover, a minor fraction of MPO is irreversibly inactivated, probably due to its reaction with the oxammonium cation resulting from tempol oxidation. Hydroxylamine 107-117 myeloperoxidase Homo sapiens 36-39 21737567-2 2011 Encapsulating nitroxides in anti-HER2 immunoliposomes at high concentrations to take advantage of the "self-quenching" phenomenon of nitroxides allows generation of robust EPR signals in HER2-overexpressing breast tumor cells with minimal background from indifferent tissues or circulating liposomes. Hydroxylamine 14-24 erb-b2 receptor tyrosine kinase 2 Homo sapiens 33-37 21737567-2 2011 Encapsulating nitroxides in anti-HER2 immunoliposomes at high concentrations to take advantage of the "self-quenching" phenomenon of nitroxides allows generation of robust EPR signals in HER2-overexpressing breast tumor cells with minimal background from indifferent tissues or circulating liposomes. Hydroxylamine 14-24 erb-b2 receptor tyrosine kinase 2 Homo sapiens 187-191 21737567-2 2011 Encapsulating nitroxides in anti-HER2 immunoliposomes at high concentrations to take advantage of the "self-quenching" phenomenon of nitroxides allows generation of robust EPR signals in HER2-overexpressing breast tumor cells with minimal background from indifferent tissues or circulating liposomes. Hydroxylamine 133-143 erb-b2 receptor tyrosine kinase 2 Homo sapiens 187-191 21737567-5 2011 Furthermore, nitroxides encapsulated in sterically stabilized anti-HER2 immunoliposomes can be delivered to HER2-overexpressing tumors at micromolar concentrations, which should be imageable by EPR. Hydroxylamine 13-23 erb-b2 receptor tyrosine kinase 2 Homo sapiens 67-71 21737567-5 2011 Furthermore, nitroxides encapsulated in sterically stabilized anti-HER2 immunoliposomes can be delivered to HER2-overexpressing tumors at micromolar concentrations, which should be imageable by EPR. Hydroxylamine 13-23 erb-b2 receptor tyrosine kinase 2 Homo sapiens 108-112 21723961-3 2011 Perfusion of the slices with l-cysteine (Cys), substrate of cystathionine beta-synthase (CBS, H(2)S synthase), could increase frequency of rhythmic respiratory discharge of the hypoglossal rootlets and prevent respiratory suppression induced by hypoxia, whereas perfusion with hydroxylamine (NH(2)OH, inhibitor of CBS) could postpone recovery of respiration from hypoxic inhibition. Hydroxylamine 277-290 cystathionine beta synthase Rattus norvegicus 60-87 21723961-3 2011 Perfusion of the slices with l-cysteine (Cys), substrate of cystathionine beta-synthase (CBS, H(2)S synthase), could increase frequency of rhythmic respiratory discharge of the hypoglossal rootlets and prevent respiratory suppression induced by hypoxia, whereas perfusion with hydroxylamine (NH(2)OH, inhibitor of CBS) could postpone recovery of respiration from hypoxic inhibition. Hydroxylamine 277-290 cystathionine beta synthase Rattus norvegicus 89-92 21723961-3 2011 Perfusion of the slices with l-cysteine (Cys), substrate of cystathionine beta-synthase (CBS, H(2)S synthase), could increase frequency of rhythmic respiratory discharge of the hypoglossal rootlets and prevent respiratory suppression induced by hypoxia, whereas perfusion with hydroxylamine (NH(2)OH, inhibitor of CBS) could postpone recovery of respiration from hypoxic inhibition. Hydroxylamine 292-299 cystathionine beta synthase Rattus norvegicus 60-87 21723961-3 2011 Perfusion of the slices with l-cysteine (Cys), substrate of cystathionine beta-synthase (CBS, H(2)S synthase), could increase frequency of rhythmic respiratory discharge of the hypoglossal rootlets and prevent respiratory suppression induced by hypoxia, whereas perfusion with hydroxylamine (NH(2)OH, inhibitor of CBS) could postpone recovery of respiration from hypoxic inhibition. Hydroxylamine 292-299 cystathionine beta synthase Rattus norvegicus 89-92 21541435-1 2011 1,3-Alternate calix[4]arene with para-phenylene spacers connecting nitroxide monoradicals and high-spin (S = 1) diradicals provides tetraradical and octaradical scaffolds that possess conformations with slow electron spin relaxation rates (1/T(1)). Hydroxylamine 67-76 spindlin 1 Homo sapiens 217-221 21558438-9 2011 Infusion of hydroxylamine (30 mM, 50 nl), a cystathionine beta-synthase inhibitor, increased ABP, HR, and RSNA. Hydroxylamine 12-25 cystathionine beta-synthase Homo sapiens 44-71 21947919-3 2011 Here, we present a novel, disulfide-linked nitroxide spin label, R1p, as an alternative to these flexible labels for PRE studies. Hydroxylamine 43-52 CD1b molecule Homo sapiens 65-68 21947919-6 2011 Together, these observations suggest that the nitroxide adopts a single, fixed position when R1p is placed at solvent-exposed alpha-helical positions, greatly simplifying the interpretation of PRE data by removing the need to account for the intrinsic flexibility of the spin label. Hydroxylamine 46-55 CD1b molecule Homo sapiens 93-96 21766795-3 2011 We discovered that, while hydroxylamine can enter the retinal binding pocket of light-activated rhodopsin, the modified hydroxylamine compounds o-methylhydroxylamine (mHA), o-ethylhydroxylamine (eHA), o-tert-butylhydroxylamine (t-bHA), and o-(carboxymethyl)hydroxylamine (cmHA) are excluded. Hydroxylamine 26-39 rhodopsin Homo sapiens 96-105 21082863-3 2010 In what we believe is the first demonstration of scanned probe detection of electron-spin resonance from a batch-fabricated tip, the cantilevers were used to observe electron-spin resonance from nitroxide spin labels in a film via force-gradient-induced shifts in cantilever resonance frequency. Hydroxylamine 195-204 TOR signaling pathway regulator Homo sapiens 124-127 21427202-7 2011 The active hydroxylamine metabolite of PR-104A, PR-104H, was also glucuronidated by UGT2B7, although with slightly lower specificity and much lower rates. Hydroxylamine 11-24 UDP glucuronosyltransferase family 2 member B7 Homo sapiens 84-90 21442105-1 2011 Amyloid nitroxyl radical (nitroxide) ligands were used to detect amyloid-beta fibrils, the main constituents of senile plaques in Alzheimer"s disease, using anisotropic ESR spectra, and were found to affect the aggregation process due to the radical functionality. Hydroxylamine 26-35 amyloid beta precursor protein Homo sapiens 65-77 21452836-1 2011 JP4-039 is a novel nitroxide conjugate capable of crossing lipid bilayer membranes and scavenging reactive oxygen species (ROS). Hydroxylamine 19-28 junctophilin 4 Homo sapiens 0-3 21279205-5 2011 A new approach is illustrated with the application to a nitroxide spin label MTSL attached to the protein sperm whale myoglobin. Hydroxylamine 56-65 myoglobin Physeter catodon 118-127 21322490-0 2011 Prediction of nitroxide spin label EPR spectra from MD trajectories: application to myoglobin. Hydroxylamine 14-23 myoglobin Physeter catodon 84-93 21322490-1 2011 We report the prediction of motional EPR spectra of the metalloprotein sperm whale myoglobin spin labelled with nitroxide directly from Molecular Dynamics (MD) simulations at the atomistic scale. Hydroxylamine 112-121 myoglobin Physeter catodon 83-92 20066490-8 2010 When targeted by anti-HER2 immunoliposomes encapsulating quenched nitroxides, Hc7 cells, which are novel HER2-overexpressing cells derived from the MCF7 breast tumor cell line, endocytose the liposomes copiously, in contrast to the parent MCF7 cells or control CV1 cells, which do not express HER2. Hydroxylamine 66-76 erb-b2 receptor tyrosine kinase 2 Homo sapiens 22-26 20813156-2 2010 We studied a HIBD 7-day-old rat model with 4 types of treatments: (1) 14 sessions of EA; (2) hydroxylamine (HA), an inhibitor of cystathionine-beta-synthase (CBS), the key enzyme of H(2)S generation; (3) both EA and HA; or (4) no treatment. Hydroxylamine 93-106 cystathionine beta synthase Rattus norvegicus 129-156 20813156-2 2010 We studied a HIBD 7-day-old rat model with 4 types of treatments: (1) 14 sessions of EA; (2) hydroxylamine (HA), an inhibitor of cystathionine-beta-synthase (CBS), the key enzyme of H(2)S generation; (3) both EA and HA; or (4) no treatment. Hydroxylamine 93-106 cystathionine beta synthase Rattus norvegicus 158-161 20066490-8 2010 When targeted by anti-HER2 immunoliposomes encapsulating quenched nitroxides, Hc7 cells, which are novel HER2-overexpressing cells derived from the MCF7 breast tumor cell line, endocytose the liposomes copiously, in contrast to the parent MCF7 cells or control CV1 cells, which do not express HER2. Hydroxylamine 66-76 CYCS pseudogene 40 Homo sapiens 78-81 20066490-8 2010 When targeted by anti-HER2 immunoliposomes encapsulating quenched nitroxides, Hc7 cells, which are novel HER2-overexpressing cells derived from the MCF7 breast tumor cell line, endocytose the liposomes copiously, in contrast to the parent MCF7 cells or control CV1 cells, which do not express HER2. Hydroxylamine 66-76 erb-b2 receptor tyrosine kinase 2 Homo sapiens 105-109 20066490-8 2010 When targeted by anti-HER2 immunoliposomes encapsulating quenched nitroxides, Hc7 cells, which are novel HER2-overexpressing cells derived from the MCF7 breast tumor cell line, endocytose the liposomes copiously, in contrast to the parent MCF7 cells or control CV1 cells, which do not express HER2. Hydroxylamine 66-76 erb-b2 receptor tyrosine kinase 2 Homo sapiens 105-109 20066490-9 2010 HER2-dependent liposomal delivery enables Hc7 cells to accumulate 750 muM nitroxide intracellularly. Hydroxylamine 74-83 erb-b2 receptor tyrosine kinase 2 Homo sapiens 0-4 20066490-9 2010 HER2-dependent liposomal delivery enables Hc7 cells to accumulate 750 muM nitroxide intracellularly. Hydroxylamine 74-83 CYCS pseudogene 40 Homo sapiens 42-45 20066490-10 2010 Through the use of phantom models, we verify that this concentration of nitroxides is more than sufficient for EPR imaging, thus laying the foundation for using EPR imaging to visualize HER2-overexpressing Hc7 tumors in animals. Hydroxylamine 72-82 erb-b2 receptor tyrosine kinase 2 Homo sapiens 186-190 20066490-10 2010 Through the use of phantom models, we verify that this concentration of nitroxides is more than sufficient for EPR imaging, thus laying the foundation for using EPR imaging to visualize HER2-overexpressing Hc7 tumors in animals. Hydroxylamine 72-82 CYCS pseudogene 40 Homo sapiens 206-209 20886156-6 2010 Split rhodopsin was resistant to hydroxylamine and activated transducin upon light absorption similarly to wild-type rhodopsin, but was readily disassembled by photobleaching. Hydroxylamine 33-46 rhodopsin Bos taurus 6-15 20715877-0 2010 Magnetic properties of nitroxide spin probes: reliable account of molecular motions and nonspecific solvent effects by time-dependent and time-independent approaches. Hydroxylamine 23-32 spindlin 1 Homo sapiens 33-37 20557105-0 2010 FTIR study of the photoreaction of bovine rhodopsin in the presence of hydroxylamine. Hydroxylamine 71-84 rhodopsin Bos taurus 42-51 20557105-4 2010 This suggests that activation of rhodopsin creates a specific reaction channel for hydroxylamine or loosens the chromophore binding pocket. Hydroxylamine 83-96 rhodopsin Bos taurus 33-42 20715877-1 2010 Application of a new integrated computational approach for two widely used nitroxide spin probes allows to show unequivocally that proper account of stereoelectronic, environmental, and dynamical effects leads to magnetic properties in quantitative agreement with experimental results without the need of any empirical parameter. Hydroxylamine 75-84 spindlin 1 Homo sapiens 85-89 20536233-6 2010 In the protein p75ICD, the orientations of the two nitroxides were found to be practically uncorrelated, and therefore the distance distribution could as readily be obtained at W-band as at X-band. Hydroxylamine 51-61 nerve growth factor receptor Homo sapiens 15-21 20418384-11 2010 Treatment of MLEC with a mitochondria-targeted radical scavenger, a conjugate of hemi-gramicidin S with nitroxide, XJB-5-131, resulted in significantly lower oxidation of both CL and PS and a decrease in hyperoxia-induced changes in caspase-3 and -7 activation. Hydroxylamine 104-113 caspase 3 Mus musculus 233-249 20459047-7 2010 Acetylcholinesterase (AChE) activity in brain was determined by hydroxylamine colorimetric assay. Hydroxylamine 64-77 acetylcholinesterase Mus musculus 0-20 20546397-5 2010 The addition of 10 mM dithiothreitol (DTT) to the substrate mix, CBZ-glutaminyl glycine and hydroxylamine, revealed a 3.6-fold increase in TGase activity, likely due in part to maintenance of the catalytic cysteine residue in a reduced state. Hydroxylamine 92-105 transglutaminase 1 Homo sapiens 139-144 20459047-7 2010 Acetylcholinesterase (AChE) activity in brain was determined by hydroxylamine colorimetric assay. Hydroxylamine 64-77 acetylcholinesterase Mus musculus 22-26 19960064-3 2010 Calibrations are given, in terms of the Block-Walker reaction field and local proton donor concentration, for the nitroxides that are commonly used in spin labeling of lipids and proteins. Hydroxylamine 114-124 spindlin 1 Homo sapiens 151-155 19997042-10 2010 CONCLUSION: These studies indicate that although novel cSNPs in CYB5A and CYB5R3 are associated with significantly altered protein expression and/or hydroxylamine reduction activities, these low-frequency cSNPs seem to only minimally impact overall observed phenotypic variability. Hydroxylamine 149-162 cytochrome b5 type A Homo sapiens 64-69 19997042-10 2010 CONCLUSION: These studies indicate that although novel cSNPs in CYB5A and CYB5R3 are associated with significantly altered protein expression and/or hydroxylamine reduction activities, these low-frequency cSNPs seem to only minimally impact overall observed phenotypic variability. Hydroxylamine 149-162 cytochrome b5 reductase 3 Homo sapiens 74-80 20560551-5 2009 The EPR spectrum of the CAT1@CB7 complex consisting of three lines suggested that probe CAT1 is associated with host CB7 such that the nitroxide part is exposed to water. Hydroxylamine 135-144 GIT ArfGAP 1 Homo sapiens 24-28 20560551-5 2009 The EPR spectrum of the CAT1@CB7 complex consisting of three lines suggested that probe CAT1 is associated with host CB7 such that the nitroxide part is exposed to water. Hydroxylamine 135-144 GIT ArfGAP 1 Homo sapiens 88-92 20560551-10 2009 The coupling constant for the seven-line spectrum for (14)N-substituted CAT1 is 5 G, and that for the four-line spectrum for (15)N-substituted CAT1 is 7.15 G. The only manner by which we could reproduce the observed spectra by simulation for both (14)N- and (15)N-substituted CAT1@CB8 was by assuming a spin exchange among three nitroxide radicals. Hydroxylamine 329-338 GIT ArfGAP 1 Homo sapiens 72-76 19568955-4 2009 The relation of these observables to the electronic structure is sketched using the nitroxide group of spin labels as a simple example. Hydroxylamine 84-93 spindlin 1 Homo sapiens 103-107 19997042-0 2010 Cytochrome b5 and NADH cytochrome b5 reductase: genotype-phenotype correlations for hydroxylamine reduction. Hydroxylamine 84-97 cytochrome b5 type A Homo sapiens 0-13 19997042-0 2010 Cytochrome b5 and NADH cytochrome b5 reductase: genotype-phenotype correlations for hydroxylamine reduction. Hydroxylamine 84-97 cytochrome b5 type A Homo sapiens 23-36 20043646-4 2010 Biotinylation of Michael-type HNE adducts using an aldehyde-reactive hydroxylamine-functionalized probe (aldehyde-reactive probe, ARP) and subsequent enrichment facilitated the identification and site-specific assignment of the modifications by LC-MS/MS analysis. Hydroxylamine 69-82 cysteine rich secretory protein 1 Homo sapiens 130-133 19804973-0 2009 Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. Hydroxylamine 30-39 albumin Homo sapiens 67-80 19804973-2 2009 A new strategy to evaluate accessibility of antioxidants to radical proteins has been developed using nitroxide prefluorescent probes anchored into human serum albumin (HSA). Hydroxylamine 102-111 albumin Homo sapiens 154-167 19631409-5 2009 The H(2)S synthesis in all these cell types was inhibited by the CBS inhibitor hydroxylamine, but not by the CGL inhibitor propargylglycine (PAG). Hydroxylamine 79-92 cystathionine beta-synthase Homo sapiens 65-68 19756290-6 2009 In all the cases analysed, the enhancement of both SEF and SERS spectra, is larger for hydroxylamine hydrochloride than that for sodium citrate. Hydroxylamine 87-114 interleukin 17 receptor D Homo sapiens 51-54 19756290-6 2009 In all the cases analysed, the enhancement of both SEF and SERS spectra, is larger for hydroxylamine hydrochloride than that for sodium citrate. Hydroxylamine 87-114 seryl-tRNA synthetase 2, mitochondrial Homo sapiens 59-63 19779106-0 2009 The mitochondria-targeted nitroxide JP4-039 augments potentially lethal irradiation damage repair. Hydroxylamine 26-35 junctophilin 4 Mus musculus 36-39 19779106-1 2009 It was unknown if a mitochondria-targeted nitroxide (JP4-039) could augment potentially lethal damage repair (PLDR) of cells in quiescence. Hydroxylamine 42-51 junctophilin 4 Mus musculus 53-56 19379130-0 2009 Inhibition of myeloperoxidase-mediated hypochlorous acid production by nitroxides. Hydroxylamine 71-81 myeloperoxidase Homo sapiens 14-29 19660142-10 2009 Furthermore, the CBS inhibitor hydroxylamine markedly attenuated the abdominal withdrawal reflex scores in response to colorectal distention in rats with CVH. Hydroxylamine 31-44 cystathionine beta synthase Rattus norvegicus 17-20 19379130-5 2009 However, we show here that nitroxides can also potently inhibit MPO-mediated HOCl production, with the nitroxide 4-aminoTEMPO inhibiting HOCl production by MPO and by neutrophils with IC50 values of approx. Hydroxylamine 27-37 myeloperoxidase Homo sapiens 64-67 19379130-5 2009 However, we show here that nitroxides can also potently inhibit MPO-mediated HOCl production, with the nitroxide 4-aminoTEMPO inhibiting HOCl production by MPO and by neutrophils with IC50 values of approx. Hydroxylamine 27-36 myeloperoxidase Homo sapiens 64-67 19379130-8 2009 Inhibition was shown to involve one-electron oxidation of the nitroxides by the compound I form of MPO and accumulation of compound II. Hydroxylamine 62-72 myeloperoxidase Homo sapiens 99-102 19379130-12 2009 Overall, these data indicate that nitroxides have considerable promise as therapeutic agents for the inhibition of MPO-mediated damage in inflammatory diseases. Hydroxylamine 34-44 myeloperoxidase Homo sapiens 115-118 19290661-2 2009 One of the most powerful approaches to measure local water dynamics within 5 A distances is to utilize the modulation of the nuclear spin relaxation rate of water protons through their time-dependent dipolar coupling to paramagnetic probes, here nitroxide spin labels. Hydroxylamine 246-255 spindlin 1 Homo sapiens 133-137 19449826-0 2009 Spin scavenging analysis of myoglobin protein-centered radicals using stable nitroxide radicals: characterization of oxoammonium cation-induced modifications. Hydroxylamine 77-86 myoglobin Equus caballus 28-37 19290661-2 2009 One of the most powerful approaches to measure local water dynamics within 5 A distances is to utilize the modulation of the nuclear spin relaxation rate of water protons through their time-dependent dipolar coupling to paramagnetic probes, here nitroxide spin labels. Hydroxylamine 246-255 spindlin 1 Homo sapiens 256-260 19245212-5 2009 These results indicate the advantages of combining an antioxidant nitroxide or nitroxide precursor with a PARP inhibitor molecule to decrease or eliminate the deleterious processes initiated by reactive oxygen and reactive nitrogen species (ROS and RNS). Hydroxylamine 66-75 FAM20C golgi associated secretory pathway kinase Homo sapiens 249-252 19245212-5 2009 These results indicate the advantages of combining an antioxidant nitroxide or nitroxide precursor with a PARP inhibitor molecule to decrease or eliminate the deleterious processes initiated by reactive oxygen and reactive nitrogen species (ROS and RNS). Hydroxylamine 79-88 FAM20C golgi associated secretory pathway kinase Homo sapiens 249-252 19231866-2 2009 The (2)BPNO(*) radical is connected to the PDI with the nitroxide and imide nitrogen atoms either para (1) or meta (3) to one another, as well as through a second intervening p-phenylene spacer (2). Hydroxylamine 56-65 peptidyl arginine deiminase 1 Homo sapiens 43-46 19243953-1 2009 By combining the structural features of acridone based anti-cancer drugs (like amsacrine) and MDR modulator propafenone, acridones with hydroxyl amine chain at N-10 have been designed and synthesized. Hydroxylamine 136-150 nuclear receptor subfamily 4 group A member 1 Homo sapiens 160-164 19357430-3 2009 Here it is shown that tobacco cell suspensions emitted NO when hydroxylamine (HA) or salicylhydroxamate (SHAM), a frequently used AOX inhibitor, was added. Hydroxylamine 63-76 ubiquinol oxidase 1, mitochondrial Nicotiana tabacum 130-133 19115960-3 2009 Here we describe the introduction of nitroxide EPR probes into calmodulin by means of site-directed spin labeling. Hydroxylamine 37-46 calmodulin 1 Homo sapiens 63-73 19728302-9 2009 The TOAC-promoted intramolecular fluorescence quenching was more pronounced for TOAC3-AII because of the proximity between the nitroxide and Tyr4. Hydroxylamine 127-136 angiotensinogen Homo sapiens 86-89 19199809-0 2009 Determination of cytochrome c and other heme proteins using the reduction wave of mercury protoporphyrin IX groups generated by a hydroxylamine induced replacement reaction. Hydroxylamine 130-143 cytochrome c, somatic Homo sapiens 17-29 19357430-3 2009 Here it is shown that tobacco cell suspensions emitted NO when hydroxylamine (HA) or salicylhydroxamate (SHAM), a frequently used AOX inhibitor, was added. Hydroxylamine 78-80 ubiquinol oxidase 1, mitochondrial Nicotiana tabacum 130-133 18399918-0 2008 Quantitation of the effect of hydroxylamine on rhodopsin palmitylation. Hydroxylamine 30-43 rhodopsin Bos taurus 47-56 26620180-2 2008 The technique requires attachment of nitroxide spin labels to the nucleotides, which may possibly perturb its conformation. Hydroxylamine 37-46 spindlin 1 Homo sapiens 47-51 26620180-3 2008 To study to what extent nitroxide spin labels may affect RNA structure, all-atom molecular dynamics simulations in explicit solvent are performed for six double-labeled RNA duplexes. Hydroxylamine 24-33 spindlin 1 Homo sapiens 34-38 26620180-4 2008 A new parametrization of the force field for the nitroxide spin label is developed, which leads to intramolecular distances that are in good agreement with experimental results. Hydroxylamine 49-58 spindlin 1 Homo sapiens 59-63 18765684-1 2008 Sulfamethoxazole is metabolized by microsomal CYP2C9 to a hydroxylamine that is thought to be responsible for the relatively high incidence of hypersensitivity reactions associated with the drug. Hydroxylamine 58-71 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 46-52 18712869-3 2008 The nitroxides proved to be hundreds of times more effective at inducing relaxation among the spin levels of o-H 2 than they are in bringing about transitions between p-H 2 and the levels of o-H 2. Hydroxylamine 4-14 polyhomeotic homolog 2 Homo sapiens 167-172 18685102-5 2008 In this study, we fed IRP2(-/-) mice a diet supplemented with a stable nitroxide, Tempol, and showed that the progression of neuromuscular impairment was markedly attenuated. Hydroxylamine 71-80 iron responsive element binding protein 2 Mus musculus 22-26 18399918-4 2008 Therefore, it is important to quantitate the stability of rhodopsin palmitates to hydroxylamine, which is a widely utilized reagent in biochemical preparations of the apoprotein. Hydroxylamine 82-95 rhodopsin Bos taurus 58-67 18399918-6 2008 Our data show that both of the bovine rhodopsin palmitates are labile to hydroxylamine, with significant depalmitylation occurring at concentrations of >or=100 mM, with an EC(50) of 220 mM L(-1). Hydroxylamine 73-86 rhodopsin Bos taurus 38-47 18837414-5 2008 Subsequently, the metastasis suppressor gene nm23-H1 cDNA (without the stop codon) was cloned into vector PBV223 in frame with the 6-histidine sequence, hydroxylamine and thrombin cleavage sites. Hydroxylamine 153-166 NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens 45-52 18490656-5 2008 For inactive rhodopsin, it was possible to find a globally minimized arrangement of nitroxide locations that simultaneously satisfied the crystal structure of rhodopsin (Protein Data Bank entry 1GZM), the experimentally measured distance data, and the known rotamers of the nitroxide side chain. Hydroxylamine 84-93 rhodopsin Homo sapiens 13-22 20641525-25 2004 Inside cells, nitroxides are reduced to hydroxylamine by cellular antioxidants such as ascorbate, thioredoxin, reductase, ubiquinol, NADPH and GSH. Hydroxylamine 14-24 thioredoxin Homo sapiens 98-109 20641525-25 2004 Inside cells, nitroxides are reduced to hydroxylamine by cellular antioxidants such as ascorbate, thioredoxin, reductase, ubiquinol, NADPH and GSH. Hydroxylamine 40-53 thioredoxin Homo sapiens 98-109 20641530-25 2004 Inside cells, nitroxides are reduced to hydroxylamine by cellular antioxidants such as ascorbate, thioredoxin, reductase, ubiquinol, NADPH and GSH. Hydroxylamine 14-24 thioredoxin Homo sapiens 98-109 20641530-25 2004 Inside cells, nitroxides are reduced to hydroxylamine by cellular antioxidants such as ascorbate, thioredoxin, reductase, ubiquinol, NADPH and GSH. Hydroxylamine 40-53 thioredoxin Homo sapiens 98-109 20641553-25 2004 Inside cells, nitroxides are reduced to hydroxylamine by cellular antioxidants such as ascorbate, thioredoxin, reductase, ubiquinol, NADPH and GSH . Hydroxylamine 14-24 thioredoxin Homo sapiens 98-109 20641553-25 2004 Inside cells, nitroxides are reduced to hydroxylamine by cellular antioxidants such as ascorbate, thioredoxin, reductase, ubiquinol, NADPH and GSH . Hydroxylamine 40-53 thioredoxin Homo sapiens 98-109 18959025-3 2008 The inhibiting effect of nitroxide compounds on oxidation of linoleic acid or linoleic alcohol by 5-lipoxygenase depends on SDS concentration. Hydroxylamine 25-34 5-lipoxygenase Solanum tuberosum 98-112 18447510-0 2008 Simulating electron spin resonance spectra of nitroxide spin labels from molecular dynamics and stochastic trajectories. Hydroxylamine 46-55 spindlin 1 Homo sapiens 20-24 18447510-0 2008 Simulating electron spin resonance spectra of nitroxide spin labels from molecular dynamics and stochastic trajectories. Hydroxylamine 46-55 spindlin 1 Homo sapiens 56-60 18447510-1 2008 Simulating electron spin resonance spectra of nitroxide spin labels from motional models is necessary for the quantitative analysis of experimental spectra. Hydroxylamine 46-55 spindlin 1 Homo sapiens 20-24 18447510-1 2008 Simulating electron spin resonance spectra of nitroxide spin labels from motional models is necessary for the quantitative analysis of experimental spectra. Hydroxylamine 46-55 spindlin 1 Homo sapiens 56-60 18490656-5 2008 For inactive rhodopsin, it was possible to find a globally minimized arrangement of nitroxide locations that simultaneously satisfied the crystal structure of rhodopsin (Protein Data Bank entry 1GZM), the experimentally measured distance data, and the known rotamers of the nitroxide side chain. Hydroxylamine 84-93 rhodopsin Homo sapiens 159-168 18490656-5 2008 For inactive rhodopsin, it was possible to find a globally minimized arrangement of nitroxide locations that simultaneously satisfied the crystal structure of rhodopsin (Protein Data Bank entry 1GZM), the experimentally measured distance data, and the known rotamers of the nitroxide side chain. Hydroxylamine 274-283 rhodopsin Homo sapiens 13-22 17929861-2 2007 In this paper, we use large-scale molecular dynamics simulations to investigate the position and behavior of nitroxide spin labels attached to stearic acid molecules in dipalmitoylphosphatidylcholine (DPPC) bilayers. Hydroxylamine 109-118 spindlin 1 Homo sapiens 119-123 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-0 2008 Impact of electron-electron spin interaction on electron spin relaxation of nitroxide diradicals and tetraradical in glassy solvents between 10 and 300 k. To determine the impact of electron-electron spin-spin interactions on electron spin relaxation rates, 1/T1 and 1/Tm were measured for nitroxide monoradical, diradical, and tetraradical derivatives of 1,3-alternate calix[4]arenes, for two pegylated high-spin nitroxide diradicals, and for an azine-linked nitroxide diradical. Hydroxylamine 76-85 spindlin 1 Homo sapiens 57-61 18284225-1 2008 The synthesis and characterization by SQUID (superconducting quantum interference device) magnetometry of one of the high-spin diradicals, in which nitroxides are conformationally constrained to be coplanar with the m-phenylene unit, is reported. Hydroxylamine 148-158 spindlin 1 Homo sapiens 122-126 18201837-11 2008 Infusion of the CBS inhibitors aminooxyacetic acid (10 mM) and hydroxylamine (20 mM) increased MAP but did not block the effects of infusion of 200 microM NaHS. Hydroxylamine 63-76 cystathionine beta synthase Rattus norvegicus 16-19 18288354-4 2008 The activities of lactate dehydrogenase (LDH) in effluent, Ca(2+)-ATPase, Na(+)-K(+)-ATPase and succinate dehydrogenase (SDH) in mitochondria were measured with colorimetry method; superoxide dismutase (SOD) activity was measured with hydroxylamine method and malondialdehyde (MDA) content in myocardial tissues was measured with TBA method. Hydroxylamine 235-248 serine dehydratase Rattus norvegicus 121-124 18198902-3 2008 A pargyline based nitroxide spin labeled irreversible inhibitor (ParSL) was used as a MAO active site specific spin probe to measure intersubunit distances in detergent (octyl beta-d-glucopyranoside, OGP) purified and OMM bound forms by a pulsed dipolar ESR spectroscopic (PDS) technique. Hydroxylamine 18-27 oviductal glycoprotein 1 Homo sapiens 200-203 17622936-0 2007 Discovery and characterization of a cytochrome b5 variant in humans with impaired hydroxylamine reduction capacity. Hydroxylamine 82-95 cytochrome b5 type A Homo sapiens 36-49 18062223-7 2007 The enzyme exhibited activity over a broad pH range from pH 5.0 to pH 11.0 and temperature range from 20 degrees C to 60 degrees C. The catalase activity was inhibited by 3-amino-1,2,4-triazole, cyanide, azide, and hydroxylamine. Hydroxylamine 215-228 Rru_A1356 Rhodospirillum rubrum ATCC 11170 136-144 17918963-9 2007 However, unlike wild-type rhodopsin, the covalent linkage of the ligand can be attacked by hydroxylamine in the dark. Hydroxylamine 91-104 rhodopsin Homo sapiens 26-35 17665970-5 2007 The in vivo ESR signal decay of carbamoyl-PROXYL, which is related to the conversion of the nitroxyl radical to hydroxylamine, was enhanced in the inoculated footpads but not in the reference one. Hydroxylamine 112-125 esterase 5 regulator Mus musculus 12-15 17622936-10 2007 CONCLUSIONS: These data indicate that a naturally occurring variant in cyt b5, T60A, leads to modestly altered affinity for hydroxylamine substrates and dramatically reduced cyt b5 expression. Hydroxylamine 124-137 cytochrome b5 type A Homo sapiens 71-77 17555520-7 2007 Slow skeletal muscle actin can be distinguished on the basis of mass, hydroxylamine cleavage and electrophoretic mobility at alkaline pH in the presence of 8 m urea. Hydroxylamine 70-83 actin Oryctolagus cuniculus 21-26 17344312-5 2007 In transfected fibroblasts, biochemical evidence showed that Kv1.5 is posttranslationally modified on both the NH(2) and COOH termini via hydroxylamine-sensitive thioester bonds. Hydroxylamine 138-151 potassium voltage-gated channel subfamily A member 5 Homo sapiens 61-66 17385063-4 2007 Hydroxylamine hypotension was enhanced by the SSAO inhibitor isoniazid and the SSAO substrate methylamine, a pattern shared by hydralazine. Hydroxylamine 0-13 amine oxidase copper containing 2 Homo sapiens 46-50 17385063-6 2007 It was concluded that hydroxylamine exerts hypotension partly through conversion to nitric oxide and partly by a "hydralazine-like" mechanism involving SSAO inhibition. Hydroxylamine 22-35 amine oxidase copper containing 2 Homo sapiens 152-156 17450323-2 2007 A bacterial expression vector containing AHL cDNA was randomly mutagenized with hydroxylamine and transformed into the E. coli cysteine auxotrophic mutant cysQ. Hydroxylamine 80-93 HAL2-like protein Arabidopsis thaliana 41-44 17066250-2 2007 In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, P(late), hydroxylamine mutagenesis was performed, resulting in almost saturating mutagenesis of alt. Hydroxylamine 123-136 RinA family protein Lactococcus phage TP901-1 48-51 17066250-2 2007 In order to identify amino acid residues in the Alt protein required for activation of the TP901-1 late promoter, P(late), hydroxylamine mutagenesis was performed, resulting in almost saturating mutagenesis of alt. Hydroxylamine 123-136 RinA family protein Lactococcus phage TP901-1 210-213 17385063-4 2007 Hydroxylamine hypotension was enhanced by the SSAO inhibitor isoniazid and the SSAO substrate methylamine, a pattern shared by hydralazine. Hydroxylamine 0-13 amine oxidase copper containing 2 Homo sapiens 79-83 17452362-4 2007 Application of the recently developed ACE chemistry presented the main advantage to limit the reduction of the nitroxide to an amine during the oligonucleotide automated synthesis and thus to increase substantially the reliability of the synthesis and the yield of labeled oligonucleotides. Hydroxylamine 111-120 angiotensin I converting enzyme Homo sapiens 38-41 17285863-2 2006 METHODS: In an acidic solution, GHB was converted to GBL, which reacted with hydroxylamine hydrochloride in presence of sodium hydroxide, forming hydroxamate. Hydroxylamine 77-104 MTOR associated protein, LST8 homolog Homo sapiens 53-56 16809339-3 2006 Determining the relative accessibility of the nitroxide-tagged amino acid side chains for the solubilized COX-2 mutants, or COX-2 reconstituted into liposomes to nonpolar (oxygen) and polar (NiEDDA or CrOx) paramagnetic reagents allowed us to map the topology of COX-2 interaction with the lipid bilayer. Hydroxylamine 46-55 prostaglandin-endoperoxide synthase 2 Homo sapiens 106-111 17022940-4 2006 The 1/deltaH of the central (14N hyperfine) component (M(I) = 0) in the ESR spectrum of spin-labeled moPrP(C) was measured as a mobility parameter of nitroxide residues (R1). Hydroxylamine 150-159 esterase 5 regulator Mus musculus 72-75 16707206-1 2006 Five singly spin labeled side chains at surface sites in the C-terminal domain of RGL2 protein have been analyzed to investigate the general relationship between nitroxide side chain mobility and protein structure. Hydroxylamine 162-171 ral guanine nucleotide dissociation stimulator like 2 Homo sapiens 82-86 16934683-0 2006 Dramatic extension of tumor latency and correction of neurobehavioral phenotype in Atm-mutant mice with a nitroxide antioxidant. Hydroxylamine 106-115 ataxia telangiectasia mutated Mus musculus 83-86 16910783-1 2006 Nitrite (NO(2)-), N (G)-hydroxy-L-arginine (NOHA), and hydroxylamine (NH(2)OH) are products of nitric oxide synthase (NOS) activity and can also be formed by secondary reactions of nitric oxide (NO). Hydroxylamine 55-68 nitric oxide synthase 2 Homo sapiens 95-116 16698792-5 2006 The EPR spectra of nitroxide probes at positions 133 and 146 exhibit spin coupling, indicating that these positions are proximal to an apoA-I paired counterpart on the perimeter of rHDL. Hydroxylamine 19-28 apolipoprotein A1 Homo sapiens 135-141 16910783-1 2006 Nitrite (NO(2)-), N (G)-hydroxy-L-arginine (NOHA), and hydroxylamine (NH(2)OH) are products of nitric oxide synthase (NOS) activity and can also be formed by secondary reactions of nitric oxide (NO). Hydroxylamine 70-77 nitric oxide synthase 2 Homo sapiens 95-116 16728955-10 2006 Sodium cyanide, sodium azide, and hydroxylamine, all of which are known heme protein inhibitors, inhibited catalase activity by 50% at concentrations of 11.5 microM, 0.52 microM, and 0.11 microM, respectively. Hydroxylamine 34-47 hemE Rhodospirillum rubrum ATCC 11170 72-76 16142694-6 2006 The ADP-ribosyl-elongation factor 2 adduct which formed upon binding of free ADP-ribose was resistant to neutral NH2OH, but decomposed almost completely upon treatment with NaOH. Hydroxylamine 113-118 eukaryotic translation elongation factor 2 Homo sapiens 16-35 16634635-1 2006 Nitroxide sensors were placed in rhodopsin at sites 140, 227, 250, and 316 to monitor the dynamics and conformation of the receptor at the cytoplasmic surface in solutions of dodecyl maltoside (DM), digitonin, and phospholipid bilayers of two compositions. Hydroxylamine 0-9 rhodopsin Homo sapiens 33-42 16620092-0 2006 Synthesis and structure of a nucleoside with pi-conjugated nitroxide spin label forming a one-dimensional ferromagnetic chain. Hydroxylamine 59-68 spindlin 1 Homo sapiens 69-73 16728955-10 2006 Sodium cyanide, sodium azide, and hydroxylamine, all of which are known heme protein inhibitors, inhibited catalase activity by 50% at concentrations of 11.5 microM, 0.52 microM, and 0.11 microM, respectively. Hydroxylamine 34-47 Rru_A1356 Rhodospirillum rubrum ATCC 11170 107-115 16507248-2 2006 METHODS: The HDalpha gene fragment with hydroxylamine cleavage site was synthesized, and then cloned into pBV220-IL-4 vector to construct pBV220-IL-4-HDalpha. Hydroxylamine 40-53 interleukin 4 Homo sapiens 145-149 16460036-5 2006 This was demonstrated by illumination of dark homogenates and treatments of illuminated homogenates with 11-cis-retinal and hydroxylamine prior to the AMP-PNP incubation and by measurement of the GCAP2 concentration required for 50% activation. Hydroxylamine 124-137 guanylate cyclase activator 1B Homo sapiens 196-201 16507248-4 2006 After fusion protein was cleaved to remove IL-4 by hydroxylamine, purification and renaturation was performed. Hydroxylamine 51-64 interleukin 4 Homo sapiens 43-47 16085771-0 2005 The hydroxylamine reaction of sensory rhodopsin II: light-induced conformational alterations with C13=C14 nonisomerizable pigment. Hydroxylamine 4-17 rhodopsin Homo sapiens 38-47 16212933-5 2005 Electron paramagnetic resonance spectroscopic spin counting analysis of proteoliposomes formed with nitroxide spin-labeled COX-2 gave a nearly identical phospholipid:COX ratio, confirming that incorporation had no effect on enzyme activity, and demonstrating that the efficiency of protein incorporation is sufficient for EPR spectroscopic analysis. Hydroxylamine 100-109 mitochondrially encoded cytochrome c oxidase II Homo sapiens 123-128 16328739-4 2005 However, the nitroxide group in GroEL-bound pmAAT showed either intermediate or high mobility depending on the spin probe used. Hydroxylamine 13-22 heat shock protein family D (Hsp60) member 1 Homo sapiens 32-37 16328739-5 2005 Power saturation experiments indicated that the accessibility of the nitroxide side chain to Ni(EDDA) in the GroEL-pmAAT complex was higher than in the native state when in position 166 but lower when at position -19. Hydroxylamine 69-78 heat shock protein family D (Hsp60) member 1 Homo sapiens 109-114 16085771-3 2005 Similarly to other retinal proteins, sensory rhodopsin II undergoes a bleaching reaction with hydroxylamine in the dark which is markedly catalyzed by light. Hydroxylamine 94-107 rhodopsin Homo sapiens 45-54 16122826-4 2005 Hydroxylamine (an inhibitor of cystathionine b-synthase) up-regulated the expression of c-fos and down-regulated the expression of GABA(B)R2, but did not change the expression of GABA(B)R1. Hydroxylamine 0-13 Fos proto-oncogene, AP-1 transcription factor subunit Rattus norvegicus 88-93 16122826-4 2005 Hydroxylamine (an inhibitor of cystathionine b-synthase) up-regulated the expression of c-fos and down-regulated the expression of GABA(B)R2, but did not change the expression of GABA(B)R1. Hydroxylamine 0-13 gamma-aminobutyric acid type B receptor subunit 2 Rattus norvegicus 131-140 16834250-4 2005 Under the operating conditions of the PUREX process, namely, 6 M nitric acid, the reactive forms of hydroxylamine are NH2OH, NH3OH+, and the complex NH3OH.NO3, and those of nitrous acid are NO+, H2ONO+, N2O4, N2O3, NO2, and NO. Hydroxylamine 100-113 NBL1, DAN family BMP antagonist Homo sapiens 155-158 15685617-16 2005 CONCLUSIONS: These data demonstrated that nitroxide tempo induced apoptosis and activated a caspase-mediated signaling pathway in prostate carcinoma cells. Hydroxylamine 42-51 caspase 9 Homo sapiens 92-99 15964563-3 2005 N(omega)-hydroxy-nor-L-arginine- (x=2) and hydroxylamine-induced relaxations were blunted by a NO scavenger and by inhibitors of the guanylyl cyclase pathway, but not by NO synthase or cytochrome P(450) inhibitors (except 7-ethoxyresorufin). Hydroxylamine 43-56 cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus 185-202 15901697-12 2005 AtzF reacts with malonamate and hydroxylamine to generate malonohydroxamate, potentially derived from hydroxylamine capture of an enzyme-tethered acyl group. Hydroxylamine 32-45 atzF Pseudomonas sp. ADP 0-4 15901697-12 2005 AtzF reacts with malonamate and hydroxylamine to generate malonohydroxamate, potentially derived from hydroxylamine capture of an enzyme-tethered acyl group. Hydroxylamine 102-115 atzF Pseudomonas sp. ADP 0-4 15846430-1 2005 Half sandwich complexes of titanium bearing eta1 or eta2 bound nitroxide ligands are highly active catalysts for the polymerisation of propylene to high molecular weight atactic poly(propylene). Hydroxylamine 63-72 DNA polymerase iota Homo sapiens 52-56 16851934-5 2005 The microviscosity (eta) deduced from the rotational motion of the nitroxide group of a spin probe increases modestly as a function of N in TMADS and TEADS, decreases slightly in TPADS, and decreases slightly before increasing in TBADS. Hydroxylamine 67-76 endothelin receptor type A Homo sapiens 20-23 15713667-8 2005 On the other hand, the results from the proteoliposome fusion assay, employing cysteine- and nitroxide-scanning mutants of Sso1p, suggested that the formation of the complete core is required for membrane fusion. Hydroxylamine 93-102 syntaxin Saccharomyces cerevisiae S288C 123-128 15843491-6 2005 Significant (p < 0.05) inhibition by selective P450 isoform inhibitor sulfaphenazole (2.1 microM; CYP2C9) indicated a role for CYP2C9 in the formation of the hydroxylamine. Hydroxylamine 161-174 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 101-107 15843491-6 2005 Significant (p < 0.05) inhibition by selective P450 isoform inhibitor sulfaphenazole (2.1 microM; CYP2C9) indicated a role for CYP2C9 in the formation of the hydroxylamine. Hydroxylamine 161-174 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 130-136 15843491-7 2005 Hydroxylamine formation correlated strongly with tolbutamide 4-hydroxylation (CYP2C8/9) in HLM (r = 0.76, p < or = 0.004, n = 12). Hydroxylamine 0-13 cytochrome P450 family 2 subfamily C member 8 Homo sapiens 78-84 15843491-8 2005 Fluconazole (CYP2C9/19 and CYP3A4 inhibitor at clinical concentrations) inhibited hydroxylamine formation, with one-enzyme model K(i) estimates ranging from 9 to 40 microM. Hydroxylamine 82-95 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 13-19 15843491-8 2005 Fluconazole (CYP2C9/19 and CYP3A4 inhibitor at clinical concentrations) inhibited hydroxylamine formation, with one-enzyme model K(i) estimates ranging from 9 to 40 microM. Hydroxylamine 82-95 cytochrome P450 family 3 subfamily A member 4 Homo sapiens 27-33 15888486-1 2005 We previously demonstrated that the nitroxide antioxidant tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl) increased latency to tumorigenesis and doubled (100%) the lifespan of Atm-deficient mice, a mouse model of ataxia telangiectasia, which displays accelerated oxidative damage and stress. Hydroxylamine 36-45 ataxia telangiectasia mutated Mus musculus 184-187 15788237-6 2005 Antioxidant activity of the nitroxides was evaluated by monitoring conjugated dienes (CD) formation during methyl linoleate micelles peroxidation and by measuring carbonyl content in oxidized bovine serum albumin (BSA). Hydroxylamine 28-38 albumin Homo sapiens 199-212 15772755-4 2005 We show here that charged nitroxide species can be helpful for identifying regions of the surface of the 4F1(5)F1 module pair from human fibronectin involved in peptide binding. Hydroxylamine 26-35 fibronectin 1 Homo sapiens 137-148 15533931-3 2005 ApoA-I was treated by cyanogen bromide or hydroxylamine; the resulting fragments, separated by electrophoresis or gel filtration, were tested by Western blotting or enzyme-linked immunosorbent assay for their ability to bind Hpt. Hydroxylamine 42-55 apolipoprotein A1 Homo sapiens 0-6 23045123-2 2005 In addition, b(5)R and cyt b(5) can directly catalyze the reduction of hydroxylamine and amidoxime metabolites. Hydroxylamine 71-84 cytochrome b5 type A Homo sapiens 23-31 15723621-6 2005 Recent advances on the regulation of human NAT1 activity has shown that hydroxylamine and/or nitroso intermediates of NAT1 substrates inhibit the enzyme through direct irreversible interaction with its catalytic cysteine residue. Hydroxylamine 72-85 N-acetyltransferase 1 Homo sapiens 43-47 15723621-6 2005 Recent advances on the regulation of human NAT1 activity has shown that hydroxylamine and/or nitroso intermediates of NAT1 substrates inhibit the enzyme through direct irreversible interaction with its catalytic cysteine residue. Hydroxylamine 72-85 N-acetyltransferase 1 Homo sapiens 118-122 16335269-5 2005 It was supposed that 5-lipoxygenase inhibition includes the interaction of hydrophobic nitroxide with radical intermediate formed in enzymatic process. Hydroxylamine 87-96 5-lipoxygenase Solanum tuberosum 21-35 15543780-0 2004 Nitroxide conjugate of a thermally responsive elastin-like polypeptide for noninvasive thermometry. Hydroxylamine 0-9 nuclear receptor subfamily 5 group A member 1 Homo sapiens 46-70 15477010-6 2004 A weak ESR signal for immobilized nitroxide derived from the protein was detected when a high concentration of cyt c was reacted with hypochlorite in the presence of the nitroso spin trap 2-methyl-2-nitrosopropane. Hydroxylamine 34-43 cytochrome c, somatic Homo sapiens 111-116 15326023-2 2004 As a reporter group, the nitroxide spin-label was attached at the C-terminus yielding the spin-labeled product (GAsl). Hydroxylamine 25-34 spindlin 1 Homo sapiens 35-39 15326023-2 2004 As a reporter group, the nitroxide spin-label was attached at the C-terminus yielding the spin-labeled product (GAsl). Hydroxylamine 25-34 spindlin 1 Homo sapiens 90-94 15504684-2 2004 The nitroxide spin label 3beta-doxyl-5alpha-cholestane (cholestane or CLS) was inserted into the bicelles and utilized to demonstrate the effects of macroscopic bilayer alignment through the measurement of orientational dependent hyperfine splittings. Hydroxylamine 4-13 cardiolipin synthase 1 Homo sapiens 70-73 15543780-3 2004 We present a novel noninvasive thermometry modality that combines a temperature responsive biopolymer, the elastin-like polypeptide (ELP), and nitroxide to produce an ELP-nitroxide conjugate. Hydroxylamine 143-152 nuclear receptor subfamily 5 group A member 1 Homo sapiens 167-170 15453264-5 2004 PCAM administration resulted in neuronal cell loss in CA1 area, which is closely associated with the neurotoxicity of endogenous glutamate and nitroxide itself. Hydroxylamine 143-152 carbonic anhydrase 1 Rattus norvegicus 54-57 15213104-3 2004 We tested this hypothesis by determining whether the well-described nitroxide antioxidant, tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl), acts as a chemopreventative agent in Atm mutant mice, a model of the human cancer prone syndrome ataxia-telangiectasia. Hydroxylamine 68-77 ataxia telangiectasia mutated Mus musculus 185-188 15176438-8 2004 We found that pretreatment of retina membranes with hydroxylamine (10 mM), a rhodopsin-inactivating drug, substantially (up to 60%) reduced basal G protein activity, thereby improving signal-to-noise ratio to detect agonist-stimulated G protein activation for all studied receptors. Hydroxylamine 52-65 rhodopsin Rattus norvegicus 77-86 15281811-0 2004 Nitroxides scavenge myeloperoxidase-catalyzed thiyl radicals in model systems and in cells. Hydroxylamine 0-10 myeloperoxidase Homo sapiens 20-35 15219989-0 2004 Hydroxylamine and hydrazine bind directly to the heme iron of the heme-heme oxygenase-1 complex. Hydroxylamine 0-13 heme oxygenase 1 Homo sapiens 71-87 15147270-10 2004 By random mutagenesis of Bfa1-D8(391-574) with hydroxylamine, we isolated a point mutant of D8, D8(E438K), which interacts with both Tem1p and Bub2p but cannot respond to checkpoint signals. Hydroxylamine 47-60 Bfa1p Saccharomyces cerevisiae S288C 25-29 15147270-10 2004 By random mutagenesis of Bfa1-D8(391-574) with hydroxylamine, we isolated a point mutant of D8, D8(E438K), which interacts with both Tem1p and Bub2p but cannot respond to checkpoint signals. Hydroxylamine 47-60 Ras family GTPase TEM1 Saccharomyces cerevisiae S288C 133-138 15147270-10 2004 By random mutagenesis of Bfa1-D8(391-574) with hydroxylamine, we isolated a point mutant of D8, D8(E438K), which interacts with both Tem1p and Bub2p but cannot respond to checkpoint signals. Hydroxylamine 47-60 Bub2p Saccharomyces cerevisiae S288C 143-148 14718257-0 2004 5-Hydroxytryptamine is biotransformed by CYP2C9, 2C19 and 2B6 to hydroxylamine, which is converted into nitric oxide. Hydroxylamine 65-78 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 41-47 15164760-6 2004 By covalently linking 32P-labeled cPLA2p(N)16 to GAPDH and after executing hydrolysis with hydroxylamine, the labeling was exclusively found in the C-terminal domain (aa 286-334). Hydroxylamine 91-104 phospholipase A2 group IVA Homo sapiens 34-40 14967030-1 2004 Site-directed spin labeling (SDSL), the site-specific incorporation of nitroxide spin-labels into a protein, has allowed us to investigate ligand-induced conformational changes in the ligand-binding domain of human estrogen receptor alpha (hERalpha-LBD). Hydroxylamine 71-80 estrogen receptor 1 Homo sapiens 215-238 15252536-1 2004 The eta1-hydroxylamido half-titanocene complex, CpTiCl2(TEMPO) 1, hydrolyzes extremely efficiently to generate (CpTiClO)4 and the protonated hydroxylamine. Hydroxylamine 141-154 secreted phosphoprotein 1 Homo sapiens 4-8 14718257-8 2004 In conclusion, CYP2B6, 2C9 and 2C19 biotransform 5-HT, yielding hydroxylamine, which is converted to nitric oxide in the presence of catalase. Hydroxylamine 64-77 cytochrome P450 family 2 subfamily B member 6 Homo sapiens 15-21 14718257-8 2004 In conclusion, CYP2B6, 2C9 and 2C19 biotransform 5-HT, yielding hydroxylamine, which is converted to nitric oxide in the presence of catalase. Hydroxylamine 64-77 catalase Homo sapiens 133-141 14750677-1 2004 A series of Au nanoparticles functionalised with nitroxide spin labels has been prepared and studied by EPR spectroscopy. Hydroxylamine 49-58 spindlin 1 Homo sapiens 59-63 14750677-4 2004 The samples with higher coverage of the spin label show an increasing contribution of the exchange interaction between nitroxides adsorbed in a close proximity to each other on the same nanoparticle. Hydroxylamine 119-129 spindlin 1 Homo sapiens 40-44 15509014-5 2004 Two-step extraction, using 10mM CuSO4 solution for exchangeable sorbed ions and 10-20mM hydroxylamine hydrochloride for ions bound to reducible Mn oxide phase, showed higher irreversibility of Co(II) and Ni(II) sorption on the biogenic Mn oxides while Zn(II) sorption was mostly reversible (Cu(II)-exchangeable). Hydroxylamine 88-115 mitochondrially encoded cytochrome c oxidase II Homo sapiens 193-199 14570470-1 2003 A very simple tyrosinase reaction system has been developed using borate anion as a trapping agent of catechols and hydroxylamine as an external reductant to evaluate the phenolase activity without the interference of catecholase activity. Hydroxylamine 116-129 tyrosinase Homo sapiens 14-24 14680368-8 2003 Involvement of the reductase domain of nNOS in the reduction of nilutamide was confirmed by (i) the ability of the isolated reductase domain of nNOS to catalyze the reaction and (ii) the stimulating effect of Ca(2+)/calmodulin on the accumulation of hydroxylamine and nitro anion radical. Hydroxylamine 250-263 nitric oxide synthase 1 Homo sapiens 39-43 14680368-8 2003 Involvement of the reductase domain of nNOS in the reduction of nilutamide was confirmed by (i) the ability of the isolated reductase domain of nNOS to catalyze the reaction and (ii) the stimulating effect of Ca(2+)/calmodulin on the accumulation of hydroxylamine and nitro anion radical. Hydroxylamine 250-263 nitric oxide synthase 1 Homo sapiens 144-148 14616032-6 2003 The calculations show that the protein environment sterically limits the area of the possible angular reorientations for the NO reporter group of the nitroxide (within the spin label), and this, in turn, affects the shape of the EPR spectrum. Hydroxylamine 150-159 spindlin 1 Homo sapiens 172-176 14510529-3 2003 Here, we present an analysis of the cleavage rate constants for 28 alkoxyamines carrying the styryl (PhEt) group as leaving alkyl radical in terms of polar inductive/field (sigmaL) and steric (Es) effects of the nitroxide substituents, using the Taft-Ingold equation, i.e., log(k/k0) = rhoLsigmaL + deltaEs. Hydroxylamine 212-221 sperm associated antigen 9 Homo sapiens 101-105 12729929-3 2003 The nitroxide spin probe 3beta-doxyl-5alpha-cholestane (cholestane or CLS) was inserted into the bilayer discs to demonstrate the effects of macroscopic bilayer alignment through the measurement of orientational dependent hyperfine splittings. Hydroxylamine 4-13 spindlin 1 Homo sapiens 14-18 12911269-4 2003 The results obtained show that overall: (i) the reduced forms of the nitroxide compounds are better scavengers of DPPH radical than butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BLT) but less efficient than the natural compounds; (ii) the nitroxides inhibit both linolenic acid micelles and bovine serum albumin (BSA) oxidation to similar extents as most of the other compounds in a concentration-dependent fashion. Hydroxylamine 69-78 albumin Homo sapiens 313-326 14692513-3 2003 Here we show that binding of human ETF, to MCAD, was inhibited by 2,3-butanedione and diethylpyrocarbonate (DEPC) and reversed by incubation with free arginine and hydroxylamine respectively. Hydroxylamine 164-177 acyl-CoA dehydrogenase medium chain Homo sapiens 43-47 12821667-1 2003 To probe the molecular nature of the binding pocket of a G protein-coupled receptor and the events immediately following the binding and activation, we have modified the substance P peptide, a potent agonist for the neurokinin-1 receptor, with a nitroxide spin probe specifically attached at Lys-3. Hydroxylamine 246-255 tachykinin precursor 1 Homo sapiens 170-181 12880948-4 2003 We hypothesize that, in vivo, HU is partially metabolized to hydroxylamine (HA), which quickly reacts with Hb to form methemoglobin (metHb) and HbNO. Hydroxylamine 61-74 hemoglobin subunit gamma 2 Homo sapiens 118-131 12880948-4 2003 We hypothesize that, in vivo, HU is partially metabolized to hydroxylamine (HA), which quickly reacts with Hb to form methemoglobin (metHb) and HbNO. Hydroxylamine 76-78 hemoglobin subunit gamma 2 Homo sapiens 118-131 12729929-3 2003 The nitroxide spin probe 3beta-doxyl-5alpha-cholestane (cholestane or CLS) was inserted into the bilayer discs to demonstrate the effects of macroscopic bilayer alignment through the measurement of orientational dependent hyperfine splittings. Hydroxylamine 4-13 cardiolipin synthase 1 Homo sapiens 70-73 12630898-6 2003 The expressed TNF fusion proteins can be cleaved by hydroxylamine. Hydroxylamine 52-65 tumor necrosis factor Homo sapiens 14-17 12595087-5 2003 Significant enhancement of the rates of both ultrasonically induced cell damage and nitroxide generation was demonstrated with 40-160 microM ATX-S10. Hydroxylamine 84-93 diencephalon/mesencephalon homeobox 1 Mus musculus 141-144 12523644-3 2002 Three spin-labelled NPY analogues containing the nitroxide group of the amino acid TOAC (2.2.6.6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid) as a paramagnetic probe were synthesized by solid-phase peptide synthesis. Hydroxylamine 49-58 neuropeptide Y Homo sapiens 20-23 15206796-2 2003 The interaction of photoexcited AA1 and 7-Atrp with the nitroxide group of TOAC was investigated by time resolved EPR. Hydroxylamine 56-65 AA1 Homo sapiens 32-35 12498984-7 2003 The present data suggest that nitroxides upregulate UCP-2, obviate weight gain, and decrease age-related spontaneous tumor incidence. Hydroxylamine 30-40 uncoupling protein 2 (mitochondrial, proton carrier) Mus musculus 52-57 12372608-0 2002 Dap-SL: a new site-directed nitroxide spin labeling approach for determining structure and motions in synthesized peptides and proteins. Hydroxylamine 28-37 death associated protein Homo sapiens 0-3 12372608-3 2002 The result is a spin-labeled side chain, referred to as Dap-SL, in which an amide bond forms a linker between the nitroxide and the peptide backbone. Hydroxylamine 114-123 death associated protein Homo sapiens 56-59 12196156-0 2002 Pentahaem cytochrome c nitrite reductase: reaction with hydroxylamine, a potential reaction intermediate and substrate. Hydroxylamine 56-69 cytochrome c, somatic Homo sapiens 10-22 12296741-1 2002 Cytochrome c nitrite reductase catalyzes the six-electron reduction of nitrite to ammonia without the release of potential reaction intermediates, such as NO or hydroxylamine. Hydroxylamine 161-174 cytochrome c, somatic Homo sapiens 0-12 12194934-5 2002 METHODS: PLP was removed from holoenzyme rHCYase by incubation with hydroxylamine to obtain apo-rHCYase. Hydroxylamine 68-81 pyridoxal phosphatase Homo sapiens 9-12 12090938-1 2002 A fluorophore-nitroxide free radical dual-functional probe (FN) was utilized to study the kinetics of ascorbate (AH(-)) binding to Bovine Serum Albumin (BSA). Hydroxylamine 13-23 albumin Homo sapiens 138-151 12018998-7 2002 Since nitroxides containing aromatic rings are likely to be substrates of cytochrome P450, experiments were carried out for assessing the ability of the cytochrome P450 monooxygenase system to metabolize PBN/*CH(3) and POBN/*CH(3), respectively. Hydroxylamine 6-16 cytochrome P450, family 2, subfamily j, polypeptide 3 Rattus norvegicus 153-182 12135567-1 2002 A novel human TF-1 cell apoptosis-related protein, TFAR19, cloned from a human leukemia cell line, TF-1, was first overexpressed in Escherichia coli with the sequence Met-Gly-His(6)-Gly-Thr-Asn-Gly, a hexahistidine sequence followed by a hydroxylamine cleavage site attached to its amino terminus. Hydroxylamine 238-251 programmed cell death 5 Homo sapiens 51-57 12135567-3 2002 After cleavage of the purified His(6)-tagged TFAR19 sample with hydroxylamine, highly purified untagged TFAR19 protein was then obtained through an FPLC Resource Q column. Hydroxylamine 64-77 programmed cell death 5 Homo sapiens 45-51 12076650-1 2002 The amino acid sequence of a thrombin like enzyme, named elegaxobin, isolated from the venom of Trimeresurus elegans (Sakishima-habu) was determined by Edman sequencing of the peptides, derived from digests with cyanogen bromide, hydroxylamine, achromobacter protease I, trypsin, V8 protease, and chymotrypsin. Hydroxylamine 230-243 coagulation factor II, thrombin Bos taurus 29-37 11921394-2 2002 It is shown here that organic nitroxide radicals ("spin labels") can be used to boost the sensitivity of NMR spectroscopic screening in drug discovery research. Hydroxylamine 30-39 spindlin 1 Homo sapiens 51-55 12162459-6 2002 In the present study we performed measurements of nitroxide metabolism in RIF-1 murine tumors, in vivo, and demonstrated that the rate of nitroxide decay correlated with the tumor redox environment. Hydroxylamine 50-59 replication timing regulatory factor 1 Mus musculus 74-79 12162459-6 2002 In the present study we performed measurements of nitroxide metabolism in RIF-1 murine tumors, in vivo, and demonstrated that the rate of nitroxide decay correlated with the tumor redox environment. Hydroxylamine 138-147 replication timing regulatory factor 1 Mus musculus 74-79 11604544-1 2001 The binding of a nitroxide spin-labeled analog of N-acetyllactosamine to galectin-3, a mammalian lectin of 26 kD size, is studied to map the binding sites of this small oligosaccharide on the protein surface. Hydroxylamine 17-26 spindlin 1 Homo sapiens 27-31 11747424-1 2001 Double-spin-labeled mutants of rhodopsin were prepared containing a nitroxide side chain at position 316 in the cytoplasmic surface helix H8, and a second nitroxide in the sequence of residues 60-75, which includes the cytoplasmic loop CL1 and cytoplasmic ends of helices TM1 and TM2. Hydroxylamine 68-77 rhodopsin Homo sapiens 31-40 11747424-1 2001 Double-spin-labeled mutants of rhodopsin were prepared containing a nitroxide side chain at position 316 in the cytoplasmic surface helix H8, and a second nitroxide in the sequence of residues 60-75, which includes the cytoplasmic loop CL1 and cytoplasmic ends of helices TM1 and TM2. Hydroxylamine 155-164 rhodopsin Homo sapiens 31-40 11747424-3 2001 In the dark state in solutions of dodecyl maltoside, the interspin distances are found to be consistent with structural models of the nitroxide side chain and rhodopsin, both derived from crystallography. Hydroxylamine 134-143 rhodopsin Homo sapiens 159-168 12240058-1 2001 (C5Me5)3Sm reacts with the free radical 2,2,6,6-tetramethylpiperidinyl-1-oxy (TMPO) to form (C5Me5)2 and the per nitroxide [(eta 1-ONC5H6Me4)2Sm(mu-eta 1:eta 2-ONC5H6Me4)]2. Hydroxylamine 113-122 secreted phosphoprotein 1 Homo sapiens 125-130 12240058-1 2001 (C5Me5)3Sm reacts with the free radical 2,2,6,6-tetramethylpiperidinyl-1-oxy (TMPO) to form (C5Me5)2 and the per nitroxide [(eta 1-ONC5H6Me4)2Sm(mu-eta 1:eta 2-ONC5H6Me4)]2. Hydroxylamine 113-122 secreted phosphoprotein 1 Homo sapiens 148-153 12240058-1 2001 (C5Me5)3Sm reacts with the free radical 2,2,6,6-tetramethylpiperidinyl-1-oxy (TMPO) to form (C5Me5)2 and the per nitroxide [(eta 1-ONC5H6Me4)2Sm(mu-eta 1:eta 2-ONC5H6Me4)]2. Hydroxylamine 113-122 DNA polymerase iota Homo sapiens 154-159 11689466-7 2001 Further evidence that early glycation products form in the regulatory regions of the myosin molecule is derived from the fact that there is complete reversal of motility speed after reaction with the Schiff base-cleaving agent hydroxylamine hydrochloride. Hydroxylamine 227-254 myosin heavy chain 14 Homo sapiens 85-91 11604544-1 2001 The binding of a nitroxide spin-labeled analog of N-acetyllactosamine to galectin-3, a mammalian lectin of 26 kD size, is studied to map the binding sites of this small oligosaccharide on the protein surface. Hydroxylamine 17-26 galectin 3 Homo sapiens 73-83 11531452-6 2001 In contrast, at relatively low DMPO/PS2.M-hemin complex ratios of < or =5 mol/mol, a simple nitroxide three-line EPR signal was detected largely in the absence of all other radicals. Hydroxylamine 95-104 taste 2 receptor member 64 pseudogene Homo sapiens 36-39 11434916-4 2001 The [(3)H]palmitic acid labeling of pro-TNF was eliminated by treatment with hydroxylamine, indicating that the labeling was due to palmitoylation of a cysteine residue via a thioester bond. Hydroxylamine 77-90 tumor necrosis factor Homo sapiens 36-43 11481253-0 2001 Inhibition of cytokine production and interference in IL-2 receptor-mediated Jak-Stat signaling by the hydroxylamine metabolite of sulfamethoxazole. Hydroxylamine 103-116 interleukin 2 Homo sapiens 54-58 11458375-0 2001 Spin-Labeled Dendrimers in EPR Imaging with Low Molecular Weight Nitroxides. Hydroxylamine 65-75 spindlin 1 Homo sapiens 0-4 11516220-4 2001 Similarly to NO, nitroxides also can react with superoxide anion radical (O(2)(-)), they possess superoxide dismutase (SOD) mimetic action. Hydroxylamine 17-27 superoxide dismutase 1 Homo sapiens 97-117 11516220-4 2001 Similarly to NO, nitroxides also can react with superoxide anion radical (O(2)(-)), they possess superoxide dismutase (SOD) mimetic action. Hydroxylamine 17-27 superoxide dismutase 1 Homo sapiens 119-122 11516220-7 2001 The biological investigations of the nitroxides showed their considerably lower general toxicity that could be explained with the SOD-mimetic action of the nitroxide present in their molecule. Hydroxylamine 37-47 superoxide dismutase 1 Homo sapiens 130-133 11516220-7 2001 The biological investigations of the nitroxides showed their considerably lower general toxicity that could be explained with the SOD-mimetic action of the nitroxide present in their molecule. Hydroxylamine 37-46 superoxide dismutase 1 Homo sapiens 130-133 11278313-5 2001 The linkage of palmitate to caveolin-1 was hydroxylamine-sensitive and thus presumably a thioester bond. Hydroxylamine 43-56 caveolin 1 Homo sapiens 28-38 11160411-7 2001 Finally, intracellular Rp-8-Br-cGMPS, a protein kinase G (PKG) inhibitor, blocked the hydroxylamine-induced membrane depolarization of cholinergic interneurons, whereas both okadaic acid and calyculin A, two protein phosphatase inhibitors, enhanced it, indicating that intracellular PKG and phosphatases oppositely regulate the sensitivity of striatal cholinergic interneurons to NO. Hydroxylamine 86-99 protein kinase cGMP-dependent 1 Homo sapiens 40-56 11408370-1 2001 Dapsone activates CYP2C9-mediated metabolism in various expression systems and is itself metabolized by CYP2C9 to its hydroxylamine metabolite. Hydroxylamine 118-131 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 104-110 11260720-4 2001 The structure reveals that the caspase is inhibited in the active site through a covalent thioester linkage to p35, which we confirmed by gel electrophoresis, hydroxylamine treatment and mass spectrometry experiments. Hydroxylamine 159-172 interleukin 12A Homo sapiens 111-114 11334627-4 2001 Both PKC isoforms of BLM are functional since a protein kinase C activator, TPA, increased the total hydroxylamine-resistant 32P(i) incorporation from [gamma-32P]ATP into the BLM. Hydroxylamine 101-114 protein kinase D1 Homo sapiens 5-8 11297791-0 2001 Glutathione reductase activity and chilling tolerance are induced by a hydroxylamine derivative BRX-156 in maize and soybean. Hydroxylamine 71-84 glutathione reductase 1 Zea mays 0-21 11160411-7 2001 Finally, intracellular Rp-8-Br-cGMPS, a protein kinase G (PKG) inhibitor, blocked the hydroxylamine-induced membrane depolarization of cholinergic interneurons, whereas both okadaic acid and calyculin A, two protein phosphatase inhibitors, enhanced it, indicating that intracellular PKG and phosphatases oppositely regulate the sensitivity of striatal cholinergic interneurons to NO. Hydroxylamine 86-99 protein kinase cGMP-dependent 1 Homo sapiens 58-61 11160411-7 2001 Finally, intracellular Rp-8-Br-cGMPS, a protein kinase G (PKG) inhibitor, blocked the hydroxylamine-induced membrane depolarization of cholinergic interneurons, whereas both okadaic acid and calyculin A, two protein phosphatase inhibitors, enhanced it, indicating that intracellular PKG and phosphatases oppositely regulate the sensitivity of striatal cholinergic interneurons to NO. Hydroxylamine 86-99 protein kinase cGMP-dependent 1 Homo sapiens 283-286 11181833-3 2001 Incubation of rat optic nerves in isoosmotic solutions containing 100 mM hydroxylamine (HA) pH 7.4 led to deacylation of PLP and decompaction of myelin lamellae at the level of the intraperiod line. Hydroxylamine 73-86 proteolipid protein 1 Rattus norvegicus 121-124 11181833-3 2001 Incubation of rat optic nerves in isoosmotic solutions containing 100 mM hydroxylamine (HA) pH 7.4 led to deacylation of PLP and decompaction of myelin lamellae at the level of the intraperiod line. Hydroxylamine 88-90 proteolipid protein 1 Rattus norvegicus 121-124 11181833-8 2001 Furthermore, in contrast to the native protein, PLP deacylated with either HA or DTE failed to induce the clustering of phosphatidylcholine/cholesterol vesicles in vitro. Hydroxylamine 75-77 proteolipid protein 1 Rattus norvegicus 48-51 11234341-4 2001 The attack by hydroxylamine is realized to a nearly equal degree at C-2 and C-6. Hydroxylamine 14-27 complement C2 Homo sapiens 68-79 11287679-2 2001 Circular dichroism (CD) spectra and binding affinity to IGF binding protein 3 (IGFBP3) indicated that the treatment with hydroxylamine did not disrupt the overall tertiary fold of the hormones. Hydroxylamine 121-134 insulin like growth factor binding protein 3 Homo sapiens 79-85 11163334-3 2001 We evaluated highly specific and reportedly non-toxic hydroxylamine-containing inhibitors of ODC (1-aminooxy-3-aminopropane, APA) and SAMDC (S-(5"-deoxy-5"-adenosyl)-methylthioethyl-hydroxylamine, AMA) in human colon cancer cells (Caco-2 and HT-29) in culture. Hydroxylamine 54-67 ornithine decarboxylase 1 Homo sapiens 93-96 11163334-9 2001 The hydroxylamine-containing ODC and SAMDC inhibitors APA and AMA are potent inhibitors of colon cancer cell proliferation and might be therapeutically promising in colon cancer. Hydroxylamine 4-17 ornithine decarboxylase 1 Homo sapiens 29-32 11108798-0 2000 Inactivation of human arylamine N-acetyltransferase 1 by the hydroxylamine of p-aminobenzoic acid. Hydroxylamine 61-74 N-acetyltransferase 1 Homo sapiens 22-53 11006278-2 2000 Line shape broadening resulting from spin-spin coupling of nitroxide pairs introduced into the membrane-binding helices of PGHS-2 was used to calculate the inter-helical distances and changes in these distances that occur in response to binding various ligands. Hydroxylamine 59-68 prostaglandin-endoperoxide synthase 2 Homo sapiens 123-129 11106502-4 2000 Upon absorption of a photon by rhodopsin, the fluorescence intensity increased as much as 20% at acidic pH with an apparent pK(a) of approximately 6.8 at 4 degrees C, and was sensitive to the presence of hydroxylamine. Hydroxylamine 204-217 rhodopsin Homo sapiens 31-40 10945985-0 2000 Arginine conversion to nitroxide by tetrahydrobiopterin-free neuronal nitric-oxide synthase. Hydroxylamine 23-32 nitric oxide synthase 1 Homo sapiens 61-91 10956056-4 2000 Conversion of this product to its bis-oxime derivative with hydroxylamine hydrochloride resulted in two syn- and two anti-oxime isomers that had chromatographic and mass spectral properties identical with the properties of products derived from an authentic standard of 4-oxo-2-nonenal. Hydroxylamine 60-87 synemin Homo sapiens 104-107 11025198-3 2000 Using a new highly sensitive technique that employs a fluorescamine-derivatized nitroxide, we show that low levels of NADPH-cytochrome P450 reductase (4.25 microg/ml) catalyze the production of hydroxyl radicals at very low, clinically relevant AZQ concentrations. Hydroxylamine 80-89 cytochrome p450 oxidoreductase Homo sapiens 118-149 10950853-2 2000 This major metabolite has been demonstrated to be further metabolized predominately by the flavin-containing monooxygenases (FMOs) to the secondary hydroxylamine, N-deacetyl-N-hydroxyketoconazole (N-hydroxy-DAK) by adult and postnatal rat hepatic microsomes. Hydroxylamine 148-161 triokinase and FMN cyclase Rattus norvegicus 207-210 10903760-9 2000 In vitro exposure of primary cultures of murine lung fibroblasts to a NO donor, hydroxylamine, induced a dose-dependent release of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1. Hydroxylamine 80-93 chemokine (C-X-C motif) ligand 2 Mus musculus 131-164 10903760-9 2000 In vitro exposure of primary cultures of murine lung fibroblasts to a NO donor, hydroxylamine, induced a dose-dependent release of macrophage inflammatory protein-2 and monocyte chemoattractant protein-1. Hydroxylamine 80-93 C-C motif chemokine ligand 2 Homo sapiens 169-203 10834943-0 2000 The pro-oxidative activity of SOD and nitroxide SOD mimics. Hydroxylamine 38-47 superoxide dismutase 1 Homo sapiens 48-51 10799659-11 2000 The hydroxylamine formation suggests a possible link between NO(-) generation and CGRP release from the vascular wall. Hydroxylamine 4-17 calcitonin-related polypeptide alpha Rattus norvegicus 82-86 10885457-3 2000 The hydroxylamine complex, bis(N,N-dimethylhydroxamido)hydroxooxovanadate (DMHAV), is an excellent inhibitor of the two PTPases, protein tyrosine phosphatase 1B (PTP1B) and leucocyte common antigen related phosphatase (LAR). Hydroxylamine 4-17 protein tyrosine phosphatase non-receptor type 1 Homo sapiens 129-160 10885457-3 2000 The hydroxylamine complex, bis(N,N-dimethylhydroxamido)hydroxooxovanadate (DMHAV), is an excellent inhibitor of the two PTPases, protein tyrosine phosphatase 1B (PTP1B) and leucocyte common antigen related phosphatase (LAR). Hydroxylamine 4-17 protein tyrosine phosphatase non-receptor type 1 Homo sapiens 162-167 10810293-4 2000 In isolated islets, NO derived from the intracellular NO donor hydroxylamine inhibited the activation of acid glucan-1, 4-alpha-glucosidase and its isoform acid alpha-glucosidase in parallel with inhibition of glucose-stimulated insulin release. Hydroxylamine 63-76 sucrase-isomaltase Homo sapiens 122-139 10810293-4 2000 In isolated islets, NO derived from the intracellular NO donor hydroxylamine inhibited the activation of acid glucan-1, 4-alpha-glucosidase and its isoform acid alpha-glucosidase in parallel with inhibition of glucose-stimulated insulin release. Hydroxylamine 63-76 sucrase-isomaltase Homo sapiens 161-178 10810293-4 2000 In isolated islets, NO derived from the intracellular NO donor hydroxylamine inhibited the activation of acid glucan-1, 4-alpha-glucosidase and its isoform acid alpha-glucosidase in parallel with inhibition of glucose-stimulated insulin release. Hydroxylamine 63-76 insulin Homo sapiens 229-236 10713083-12 2000 Random mutagenesis by hydroxylamine treatment and error-prone polymerase chain reaction identified several residues in this region of TraR important for interacting with TraM as well as for transcriptional activation or/and DNA binding. Hydroxylamine 22-35 transcriptional regulator TraR Agrobacterium tumefaciens 134-138 10773169-2 2000 The enhanced Zeeman splitting leads to spectra with resolved g-tensor components of the nitroxide spin label. Hydroxylamine 88-97 spindlin 1 Homo sapiens 98-102 10773169-6 2000 The enhanced sensitivity of high-field EPR with respect to anisotropic reorientational motion of nitroxides allows the characterization of different motional modes for spin labels bound to positions 167 and 170. Hydroxylamine 97-107 spindlin 1 Homo sapiens 168-172 10558865-3 1999 The spectra demonstrated that at sites C98 and C99 the mobility of the nitroxides was reduced in the presence of lysozyme, lactoferrin, but not albumin. Hydroxylamine 71-81 lysozyme Homo sapiens 113-121 10629768-4 1999 The aim of this study was to investigate the effects of treatment with the intracellular NO donor hydroxylamine on: (i) basilar arterial wall that remained in a direct contact with the clot, (ii) formation of the beta-amyloid precursor protein (beta-APP), (iii) total brain NOS activity, and (iv) neurological outcome in a "two-hemorrhage" rat SAH model. Hydroxylamine 98-111 amyloid beta precursor protein Homo sapiens 213-243 10629768-4 1999 The aim of this study was to investigate the effects of treatment with the intracellular NO donor hydroxylamine on: (i) basilar arterial wall that remained in a direct contact with the clot, (ii) formation of the beta-amyloid precursor protein (beta-APP), (iii) total brain NOS activity, and (iv) neurological outcome in a "two-hemorrhage" rat SAH model. Hydroxylamine 98-111 amyloid beta precursor protein Homo sapiens 245-253 10679621-1 2000 Linear Aib-based hexapeptides, of the general formula Ac-Toac-(Aib)(n) -Trp-(Aib)(r) -OtBu [T(Aib)(n) Trp], where n + r = 4, and Toac is a nitroxide spin-labeled C(alpha,alpha)-disubstituted glycine, were investigated by steady-state and time-resolved fluorescence measurements in different solvent media. Hydroxylamine 139-148 ANIB1 Homo sapiens 7-10 10523659-5 1999 The electrophoretic mobility of wild-type Ste18p (but not the mutant Ste18p) was sensitive to hydroxylamine treatment, consistent with palmitoyl modification at Cys 106. Hydroxylamine 94-107 Ste18p Saccharomyces cerevisiae S288C 42-48 10489118-13 1999 (7) Both the radical and its corresponding hydroxylamine, which is the reduced form of the radical, were detectable by X-band ESR method in the collected urine of mice and rats without and with an oxidizing agent, respectively. Hydroxylamine 43-56 esterase 5 regulator Mus musculus 126-129 10813890-0 2000 Thermal decomposition mechanisms of tert-alkyl peroxypivalates studied by the nitroxide radical trapping technique The thermolysis of a series of tert-alkyl peroxypivalates 1 in cumene has been investigated by using the nitroxide radical-trapping technique. Hydroxylamine 78-87 telomerase reverse transcriptase Homo sapiens 36-40 10629851-2 1999 Catalase is capable of producing NO from azide and hydroxylamine (Ignarro LJ, FASEB J 1989; 3:31-36). Hydroxylamine 51-64 catalase Rattus norvegicus 0-8 10629851-8 1999 The catalase activity of the aorta was inhibited by azide and hydroxylamine with the similar IC50 values to EC50 values for relaxation. Hydroxylamine 62-75 catalase Rattus norvegicus 4-12 10570951-2 1999 High-Mr reaction products were also detected after incubation of C1Inh with NS3 but not with NS3p; they correspond to ester-bonded complexes from their hydroxylamine lability. Hydroxylamine 152-165 serpin family G member 1 Homo sapiens 65-70 10570951-2 1999 High-Mr reaction products were also detected after incubation of C1Inh with NS3 but not with NS3p; they correspond to ester-bonded complexes from their hydroxylamine lability. Hydroxylamine 152-165 KRAS proto-oncogene, GTPase Homo sapiens 76-79 10566945-6 1999 Application of hydroxylamine to de-palmitoylate SNAP-25 resulted in a slight reduction of the immunoreactivity of SNAP-25 N terminus, while it had no effect on immunoreactivity of botulinum A cleaved SNAP-25. Hydroxylamine 15-28 synaptosome associated protein 25 Homo sapiens 48-55 10566945-6 1999 Application of hydroxylamine to de-palmitoylate SNAP-25 resulted in a slight reduction of the immunoreactivity of SNAP-25 N terminus, while it had no effect on immunoreactivity of botulinum A cleaved SNAP-25. Hydroxylamine 15-28 synaptosome associated protein 25 Homo sapiens 114-121 10566945-6 1999 Application of hydroxylamine to de-palmitoylate SNAP-25 resulted in a slight reduction of the immunoreactivity of SNAP-25 N terminus, while it had no effect on immunoreactivity of botulinum A cleaved SNAP-25. Hydroxylamine 15-28 synaptosome associated protein 25 Homo sapiens 114-121 10566945-7 1999 In contrast, application of hydroxylamine to nerve terminals where syntaxin had been cleaved by botulinum C caused a considerable reduction in SNAP-25 N-terminus immunoreactivity. Hydroxylamine 28-41 synaptosome associated protein 25 Homo sapiens 143-150 10421614-7 1999 The initial metabolite of DAK was a secondary hydroxylamine, N-deacetyl-N-hydroxyketoconazole, which was confirmed by liquid chromatography/mass spectrometry and NMR spectroscopy. Hydroxylamine 46-59 triokinase and FMN cyclase Rattus norvegicus 26-29 10423365-0 1999 Nitroxide side-chain dynamics in a spin-labeled helix-forming peptide revealed by high-frequency (139.5-GHz) EPR spectroscopy. Hydroxylamine 0-9 spindlin 1 Homo sapiens 35-39 10423365-1 1999 High-frequency electron paramagnetic resonance (EPR) spectroscopy has been performed on a nitroxide spin-labeled peptide in fluid aqueous solution. Hydroxylamine 90-99 spindlin 1 Homo sapiens 100-104 10423365-4 1999 Simulation of the temperature-dependent spectral lineshapes reveals the existence of local anisotropic motion about the nitroxide N-O bond with a motional anisotropy tau( perpendicular)/tau( parallel) ( identical with N) approaching 2.6 at 306 K. Comparison with previous work on rigidly labeled peptides suggests that the spin label is reorienting about its side-chain tether. Hydroxylamine 120-129 spindlin 1 Homo sapiens 323-327 10393098-5 1999 The histidine-modifying agent diethyl pyrocarbonate (DEPC) inhibited recombinant rat GCS expressed in bacteria; this inhibition was rapidly reversible by hydroxylamine and could be diminished by preincubation of GCS with UDP-Glc. Hydroxylamine 154-167 UDP-glucose ceramide glucosyltransferase Rattus norvegicus 85-88 10514286-6 1999 s(-1) for 4c) and thrombin (k(i)/K(I) = 7 200 M(-1).s(-1) for 3b) as demonstrated by spontaneous or hydroxylamine-accelerated reactivation, irrespective of the nature of the substituent at the 6-position. Hydroxylamine 100-113 coagulation factor II, thrombin Homo sapiens 18-26 10506572-0 1999 Cytotoxicity of sulfonamide reactive metabolites: apoptosis and selective toxicity of CD8(+) cells by the hydroxylamine of sulfamethoxazole. Hydroxylamine 106-119 CD8a molecule Homo sapiens 86-89 10434018-3 1999 We conclude that hydroxylamine acts presynaptically to counter adenosine A(1) receptor-mediated inhibition of synaptic transmission. Hydroxylamine 17-30 adenosine A1 receptor Rattus norvegicus 63-86 10421457-5 1999 Recombinant wild-type and C283A PON1 enzymes inhibited with DEPC and subsequently treated with hydroxylamine had partial restoration of activity. Hydroxylamine 95-108 paraoxonase 1 Homo sapiens 32-36 10359648-4 1999 All three ECE-1 isoforms were found to be palmitoylated via hydroxylamine-sensitive thioester bonds. Hydroxylamine 60-73 endothelin converting enzyme 1 Homo sapiens 10-15 10467955-11 1999 These results suggest that the formation clearance of dapsone hydroxylamine reflects alterations in CYP3A activity, despite the fact that it accounted for a small part of the systemic clearance of dapsone. Hydroxylamine 62-75 cytochrome P450, family 3, subfamily a, polypeptide 62 Rattus norvegicus 100-105 10359764-2 1999 By using a recently developed electron paramagnetic resonance approach together with 13 site-specifically nitroxide spin labeled C2cPLA2s and membrane-permeant and -impermeant spin relaxants, we have determined the orientation of C2cPLA2 with respect to the surface of vesicles of the phospholipid 1,2-dioleoyl-sn-glycero-3-phosphomethanol. Hydroxylamine 106-115 complement C2 Homo sapiens 230-237 9756926-4 1998 P-opsin bleached slowly in the dark (t1/2 = 2 h) in the presence of 50 mM hydroxylamine. Hydroxylamine 74-87 opsin, pineal Gallus gallus 0-7 10052942-6 1999 Serine and hydroxylamine form an alpha-aminoacrylate and an oxime with PLP in CBS, respectively. Hydroxylamine 11-24 cystathionine beta-synthase Homo sapiens 78-81 10052942-9 1999 PLP but not heme dissociates from the enzyme in the presence of hydroxylamine. Hydroxylamine 64-77 pyridoxal phosphatase Homo sapiens 0-3 10052944-13 1999 Treatment of cystathionine beta-synthase with hydroxylamine releases two PLPs after 1 day and results in complete loss of activity. Hydroxylamine 46-59 cystathionine beta-synthase Homo sapiens 13-40 11674206-5 1999 Superoxide trapping by DEPPPO gave a persistent nitroxide spin adduct, and its half-time life was measured and compared to that of the DEPMPO analogue. Hydroxylamine 48-57 spindlin 1 Homo sapiens 58-62 10027831-0 1999 Methemoglobin formation by hydroxylamine metabolites of sulfamethoxazole and dapsone: implications for differences in adverse drug reactions. Hydroxylamine 27-40 hemoglobin subunit gamma 2 Homo sapiens 0-13 10027831-8 1999 In contrast, the pharmacodynamics of hydroxylamine-induced MetHgb formation were not changed by pretreatment with the glucose 6-phosphate dehydrogenase inhibitor epiandrosterone or by compounds that alter normal antioxidant enzyme activity. Hydroxylamine 37-50 hemoglobin subunit gamma 2 Homo sapiens 59-65 10050013-9 1999 The intracellular NO donor hydroxylamine dose-dependently inhibited carbachol-induced insulin release but stimulated glucagon release only at a low concentration (3 microM). Hydroxylamine 27-40 insulin Homo sapiens 86-93 10208642-1 1999 Sulphamethoxazole undergoes CYP2C9-mediated bioactivation to a hydroxylamine. Hydroxylamine 63-76 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 28-34 9873053-2 1999 Labeling of PLD1 was abolished by treatment with hydroxylamine at neutral pH, indicating that the fatty acid is linked via thioester to the enzyme. Hydroxylamine 49-62 phospholipase D1 Homo sapiens 12-16 9873053-6 1999 Treatment of immunoprecipitated PLD1 with hydroxylamine diminished catalytic activity to background levels in a dose response manner that paralleled the removal of label from [3H]palmitate-labeled protein. Hydroxylamine 42-55 phospholipase D1 Homo sapiens 32-36 10231390-7 1999 Several temperature sensitive (ts-) mutants were independently created by the random mutagenesis of COF1 with hydroxylamine. Hydroxylamine 110-123 cofilin Saccharomyces cerevisiae S288C 100-104 10092619-10 1999 The data obtained allow us to conclude that under some conditions toxic Abeta peptides Abeta-(1-40) and Abeta-(25-35) enhance metal-catalyzed oxidation of hydroxylamine derivatives, but do not spontaneously form peptide-derived free radicals. Hydroxylamine 155-168 amyloid beta precursor protein Homo sapiens 72-77 10092619-10 1999 The data obtained allow us to conclude that under some conditions toxic Abeta peptides Abeta-(1-40) and Abeta-(25-35) enhance metal-catalyzed oxidation of hydroxylamine derivatives, but do not spontaneously form peptide-derived free radicals. Hydroxylamine 155-168 amyloid beta precursor protein Homo sapiens 87-92 10092619-10 1999 The data obtained allow us to conclude that under some conditions toxic Abeta peptides Abeta-(1-40) and Abeta-(25-35) enhance metal-catalyzed oxidation of hydroxylamine derivatives, but do not spontaneously form peptide-derived free radicals. Hydroxylamine 155-168 amyloid beta precursor protein Homo sapiens 87-92 10232828-8 1999 In conclusion, our results suggest that the secondary amine represents one of the major metabolites of nitroxides besides the hydroxylamine inside keratinocytes formed via the flavoenzyme thioredoxin reductase most probably. Hydroxylamine 103-113 peroxiredoxin 5 Homo sapiens 188-209 10232828-8 1999 In conclusion, our results suggest that the secondary amine represents one of the major metabolites of nitroxides besides the hydroxylamine inside keratinocytes formed via the flavoenzyme thioredoxin reductase most probably. Hydroxylamine 126-139 peroxiredoxin 5 Homo sapiens 188-209 10052942-6 1999 Serine and hydroxylamine form an alpha-aminoacrylate and an oxime with PLP in CBS, respectively. Hydroxylamine 11-24 pyridoxal phosphatase Homo sapiens 71-74 10357176-3 1998 Data indicating extracellular reduction of a nitroxide free radical Cat1 (1-oxy-4-trimethylamine-2,2,6,6,tetramethyl-piperidine) by hepatocytes were thought to be artefactual. Hydroxylamine 45-54 GIT ArfGAP 1 Homo sapiens 68-72 10087986-9 1998 Other enzyme activities glutathione reductase (GR), glucose 6-phosphate dehydrogenase (G6PDH), glucose phosphate isomerase and NADH methemoglobin reductase, were not or only slightly impaired by hydroxylamine or O-ethyl hydroxylamine treatment. Hydroxylamine 195-208 glucose-6-phosphate dehydrogenase Homo sapiens 87-92 9821018-4 1998 However, the activity of glutathione peroxidase (GPX) and glutathione S-transferase (GST) was strongly inhibited by hydroxylamine and its O-derivatives (O-methyl and O-ethyl hydroxylamine). Hydroxylamine 116-129 glutathione S-transferase kappa 1 Homo sapiens 58-83 9821018-4 1998 However, the activity of glutathione peroxidase (GPX) and glutathione S-transferase (GST) was strongly inhibited by hydroxylamine and its O-derivatives (O-methyl and O-ethyl hydroxylamine). Hydroxylamine 116-129 glutathione S-transferase kappa 1 Homo sapiens 85-88 9725818-8 1998 The intracellular NO donor hydroxylamine dose dependently inhibited insulin but increased glucagon secretion in K+-depolarized and amino acid-stimulated islets. Hydroxylamine 27-40 insulin Homo sapiens 68-75 9737850-4 1998 The topological orientation of PTH1R[168-198] was determined from nitroxide-radical induced relaxation of NMR signals utilizing 5- and 16-doxylstearic acid. Hydroxylamine 66-75 parathyroid hormone 1 receptor Homo sapiens 31-36 10087986-16 1998 Hydroxylamine as well as O-alkylated derivatives primarily induce methemoglobin, a process involving radical formation. Hydroxylamine 0-13 hemoglobin subunit gamma 2 Homo sapiens 66-79 9877233-11 1998 The pharmacological intracellular NO donor hydroxylamine dose-dependently inhibited insulin release stimulated by TPA. Hydroxylamine 43-56 insulin Homo sapiens 84-91 9877233-12 1998 Furthermore, a series of perifusion experiments revealed that hydroxylamine greatly inhibited carbachol-induced insulin release without affecting the 45Ca2+ -efflux pattern. Hydroxylamine 62-75 insulin Homo sapiens 112-119 9733587-10 1998 Xenopus violet opsin was sensitive to hydroxylamine in the dark, reacting with a half-time of 5 min at room temperature. Hydroxylamine 38-51 rhodopsin, gene2 L homeolog Xenopus laevis 15-20 9649399-0 1998 Effective light-induced hydroxylamine reactions occur with C13 = C14 nonisomerizable bacteriorhodopsin pigments. Hydroxylamine 24-37 homeobox C13 Homo sapiens 59-62 9521691-6 1998 Regeneration of lysine and arginine residues by hydroxylamine treatment completely restored the binding of modified LDL to MTP. Hydroxylamine 48-61 microsomal triglyceride transfer protein Homo sapiens 123-126 9721309-2 1998 In an attempt to elucidate the role of UmuD" in SOS mutagenesis, we have utilized a colorimetric papillation assay to screen for mutants of a hydroxylamine-treated, low-copy-number umuD" plasmid that are unable to promote SOS-dependent spontaneous mutagenesis. Hydroxylamine 142-155 UmuD Escherichia coli 39-44 9721309-2 1998 In an attempt to elucidate the role of UmuD" in SOS mutagenesis, we have utilized a colorimetric papillation assay to screen for mutants of a hydroxylamine-treated, low-copy-number umuD" plasmid that are unable to promote SOS-dependent spontaneous mutagenesis. Hydroxylamine 142-155 UmuD Escherichia coli 181-186 9692212-2 1998 The sequences of the TBC-1 and TBC-2 were solved by analysis of peptides derived by enzymatic digestions as well as by chemical cleavages with cyanogen bromide (CNBr), o-iodosobenzoic acid, and hydroxylamine. Hydroxylamine 194-207 TBC1 domain family member 1 Homo sapiens 21-26 9641253-4 1998 Addition of NADPH to a microsomal system containing PBN/.CCl3 presumably reduced the radical adduct to its ESR-silent hydroxylamine because no ESR signal was observed. Hydroxylamine 118-131 C-C motif chemokine ligand 3 Homo sapiens 57-61 9494117-5 1998 PKC activity assays conducted with a series of NM-peptides successively substituted with nitroxide spin labels at each residue position suggested that several residues distal to the phosphorylation site are necessary for substrate recognition. Hydroxylamine 89-98 protein kinase C alpha Bos taurus 0-3 9443873-10 1998 Using in vitro chemical probing strategies, we also show that the GAA triplet repeat adopts an unusual DNA structure, demonstrated by hyperreactivity to osmium tetroxide, hydroxylamine, and diethyl pyrocarbonate. Hydroxylamine 171-184 alpha glucosidase Homo sapiens 66-69 9461622-8 1998 Moreover, recombinant M1Pase is subject to active site-directed, hydroxylamine-reversible inhibition by the histidine-selective acylating reagent diethyl pyrocarbonate. Hydroxylamine 65-78 ETH_00027300 Eimeria tenella 22-28 9238019-7 1997 In the presence of [gamma-32P]ATP, ECA1p formed a Ca2+-dependent [32P]phosphoprotein of 106 kDa that was sensitive to hydroxylamine. Hydroxylamine 118-131 ER-type Ca2+-ATPase 1 Arabidopsis thaliana 35-40 9325149-0 1997 Hydroxylamine-induced cleavage of the asparaginyl-glycine motif in the production of recombinant proteins: the case of insulin-like growth factor I. Hydroxylamine 0-13 insulin like growth factor 1 Homo sapiens 119-147 9315611-11 1997 In the case of RA25, the drug is in the outer part of the head group interface being much exposed to the aqueous phase and being significantly less accessible to the membrane nitroxide quenchers. Hydroxylamine 175-184 RA25 Homo sapiens 15-19 9474373-7 1998 Interestingly, the blue-sensitive SWS2Ac pigment shows an exceptionally low level of sensitivity to hydroxylamine, possessing a rod pigment-like characteristic. Hydroxylamine 100-113 sws2 Anolis carolinensis 34-38 9369488-6 1997 To investigate the possibility that CD-GCAP is a post-translationally modified form of S100b, both proteins were treated with 1 M hydroxylamine which is known to deacylate proteins. Hydroxylamine 130-143 S100 calcium binding protein B Homo sapiens 87-92 9369488-8 1997 Hydroxylamine also broke down CD-GCAP into smaller fragments while leaving S100b intact. Hydroxylamine 0-13 guanylate cyclase activator 1A Homo sapiens 33-37 9369488-8 1997 Hydroxylamine also broke down CD-GCAP into smaller fragments while leaving S100b intact. Hydroxylamine 0-13 S100 calcium binding protein B Homo sapiens 75-80 9367178-0 1997 Nitric oxide formation from hydroxylamine by myoglobin and hydrogen peroxide. Hydroxylamine 28-41 myoglobin Homo sapiens 45-54 9367178-2 1997 In this study, HA and methyl-substituted hydroxylamines, N-methylhydroxylamine (NMHA) and N,N-dimethylhydroxylamine (NDMHA), have been tested for their ability to generate free diffusible nitric oxide (NO) in the presence of myoglobin (Mb) and hydrogen peroxide. Hydroxylamine 15-17 myoglobin Homo sapiens 225-234 9367178-2 1997 In this study, HA and methyl-substituted hydroxylamines, N-methylhydroxylamine (NMHA) and N,N-dimethylhydroxylamine (NDMHA), have been tested for their ability to generate free diffusible nitric oxide (NO) in the presence of myoglobin (Mb) and hydrogen peroxide. Hydroxylamine 15-17 myoglobin Homo sapiens 236-238 9278398-11 1997 The inhibited PTP1B could be partially reactivated by treatment with reducing agents such as hydroxylamine (NH2OH) and N,N"-dimethyl-N, N"-bis(mercaptoacetyl)hydrazine, indicating the presence of other oxidized forms such as sulfenic acid (Cys-SOH). Hydroxylamine 93-106 protein tyrosine phosphatase non-receptor type 1 Homo sapiens 14-19 9278398-11 1997 The inhibited PTP1B could be partially reactivated by treatment with reducing agents such as hydroxylamine (NH2OH) and N,N"-dimethyl-N, N"-bis(mercaptoacetyl)hydrazine, indicating the presence of other oxidized forms such as sulfenic acid (Cys-SOH). Hydroxylamine 108-113 protein tyrosine phosphatase non-receptor type 1 Homo sapiens 14-19 9282832-7 1997 The results suggested that tyramine was sequentially N-oxygenated in the presence of pig and human liver microsomes and cDNA-expressed FMO3 to the hydroxylamine and then to the di-N-hydroxylamine that was spontaneously dehydrated to the trans oxime. Hydroxylamine 147-160 flavin containing dimethylaniline monoxygenase 3 Homo sapiens 135-139 9245713-5 1997 In this report we show that the A33 antigen is (I) N-glycosylated, containing approximately 8 K of N-linked carbohydrate and there is no evidence for O-glycosylation, sialylation or glycophosphatidylinositol, and (ii) S-acylated in vitro, incorporating [3H] palmitic acid linked through a hydroxylamine-sensitive thioester bond. Hydroxylamine 289-302 glycoprotein A33 Homo sapiens 32-35 11669952-0 1997 Ferromagnetic Interactions between Imino Nitroxides through Diamagnetic Metal Ions: Crystal Structures, Magnetism, and Electronic Properties of [M(I)(imino nitroxide)(2)](PF(6)) (M = Cu(I) and Ag(I)). Hydroxylamine 156-165 sperm associated antigen 17 Homo sapiens 171-177 11669965-5 1997 With trace amounts of oxygen, the reaction of Co(II)(TPP) with hydroxylamine led to the formation of a stable cobalt(III)-bis(hydroxylamine) complex. Hydroxylamine 63-76 mitochondrially encoded cytochrome c oxidase II Homo sapiens 46-52 11669965-5 1997 With trace amounts of oxygen, the reaction of Co(II)(TPP) with hydroxylamine led to the formation of a stable cobalt(III)-bis(hydroxylamine) complex. Hydroxylamine 63-76 mitochondrially encoded cytochrome c oxidase III Homo sapiens 117-120 11669965-12 1997 The one-electron oxidation of the hydroxylamine complex with a M(III) porphyrin would be expected to oxidize the N-atom in the coordinated hydroxylamine. Hydroxylamine 34-47 mitochondrially encoded cytochrome c oxidase III Homo sapiens 65-68 11669965-12 1997 The one-electron oxidation of the hydroxylamine complex with a M(III) porphyrin would be expected to oxidize the N-atom in the coordinated hydroxylamine. Hydroxylamine 139-152 mitochondrially encoded cytochrome c oxidase III Homo sapiens 65-68 9200687-2 1997 Spin-labeled biotin-PEs were prepared with the nitroxide group at position C-5, C-8, C-10, C-12, or C-14 of the sn-2 chain and were incorporated at 1 mol % in lipid bilayer membranes of dimyristoylphosphatidylcholine. Hydroxylamine 47-56 spindlin 1 Homo sapiens 0-4 8951340-0 1996 Hydroxylamine and phenol-induced formation of methemoglobin and free radical intermediates in erythrocytes. Hydroxylamine 0-13 hemoglobin subunit gamma 2 Homo sapiens 46-59 9137753-2 1997 The method utilized the phenomenon that the lophine-Co(II)-H2O2 chemiluminescence (CL) reaction is enhanced in alkaline media by the addition of hydroxylammonium chloride. Hydroxylamine 145-170 mitochondrially encoded cytochrome c oxidase II Homo sapiens 52-58 9245357-0 1997 A Double-Modulation ESR Study of Internal Dynamics in the Glassy Polymer Matrix Detected by a Nitroxide Spin Probe The double-modulation ESR spin-probe technique was employed to study slow-motional dynamics in amorphous polymer matrices of polybutadiene, polyisobutylene, poly(methyl methacrylate), and paraffin. Hydroxylamine 94-103 spindlin 1 Homo sapiens 104-108 9245357-0 1997 A Double-Modulation ESR Study of Internal Dynamics in the Glassy Polymer Matrix Detected by a Nitroxide Spin Probe The double-modulation ESR spin-probe technique was employed to study slow-motional dynamics in amorphous polymer matrices of polybutadiene, polyisobutylene, poly(methyl methacrylate), and paraffin. Hydroxylamine 94-103 spindlin 1 Homo sapiens 141-145 9034235-9 1997 Cell exposure to the glutathione synthetase inhibitor buthionine-sulfoximine depleted intracellular glutathione and inhibited nitroxide reduction; exposure to dehydroascorbate or glutathione-monoethylester increased intracellular ascorbate or glutathione concentration and stimulated nitroxide reduction. Hydroxylamine 126-135 glutathione synthetase Homo sapiens 21-43 9034235-9 1997 Cell exposure to the glutathione synthetase inhibitor buthionine-sulfoximine depleted intracellular glutathione and inhibited nitroxide reduction; exposure to dehydroascorbate or glutathione-monoethylester increased intracellular ascorbate or glutathione concentration and stimulated nitroxide reduction. Hydroxylamine 284-293 glutathione synthetase Homo sapiens 21-43 8958151-6 1997 The nitroxide with a C10 chain was less effective in this system. Hydroxylamine 4-13 homeobox C10 Homo sapiens 21-24 9098095-10 1997 Bioreduction of Tempol to its corresponding hydroxylamine (which is not a radioprotector) occurred to a greater extent in RIF-1 tumor cells compared to bone marrow. Hydroxylamine 44-57 replication timing regulatory factor 1 Mus musculus 122-127 8943296-2 1996 Replacement of a highly conserved glycine residue on transmembrane (TM) helix 3 of bovine rhodopsin (Gly121) by amino acid residues with larger side chains causes a progressive blue-shift in the lambdamax value of the pigment, a decrease in thermal stability, and an increase in reactivity with hydroxylamine. Hydroxylamine 295-308 rhodopsin Bos taurus 90-99 8943311-14 1996 Deacylation of affinity-purified ASGP-Rs with hydroxylamine results in the spontaneous formation of dimers through reversible disulfide bonds, indicating that deacylation concomitantly generates free thiol groups. Hydroxylamine 46-59 mucin 4, cell surface associated Rattus norvegicus 33-37 8943311-15 1996 Reaction of hydroxylamine-treated ASGP-R with [14C]iodoacetamide resulted in the specific incorporation of radioactivity into all RHL and HHL subunits, verifying that fatty acids are attached via thioester linkages. Hydroxylamine 12-25 mucin 4, cell surface associated Rattus norvegicus 34-38 8713077-4 1996 Diethyl pyrocarbonate (DEP) inactivates cGI-PDE in a time- and concentration-dependent manner, and this effect was rapidly reversed by hydroxylamine. Hydroxylamine 135-148 phosphodiesterase 3A Homo sapiens 40-47 8956097-3 1996 Exceptionally rapid methemoglobin formers-hydroxylamine hydrochloride (HH) and dimethylaminophenol (DMAP)-are usually able to prevent the lethal effect of cyanide following intramuscular injections in doses sufficient to induce 20% methemoglobin (HH = 20 mg kg-1 and DMAP = 2 mg kg-1). Hydroxylamine 42-69 hemoglobin subunit gamma 2 Homo sapiens 20-33 8956097-3 1996 Exceptionally rapid methemoglobin formers-hydroxylamine hydrochloride (HH) and dimethylaminophenol (DMAP)-are usually able to prevent the lethal effect of cyanide following intramuscular injections in doses sufficient to induce 20% methemoglobin (HH = 20 mg kg-1 and DMAP = 2 mg kg-1). Hydroxylamine 42-69 hemoglobin subunit gamma 2 Homo sapiens 232-245 8911919-0 1996 Hydroxylamine, a nitric oxide donor, inhibits insulin release and activates K+ATP channels. Hydroxylamine 0-13 insulin Homo sapiens 46-53 8911919-2 1996 Hydroxylamine provoked a concentration-dependent inhibition of the glucose-induced insulin release. Hydroxylamine 0-13 insulin Homo sapiens 83-90 8911919-6 1996 Hydroxylamine decreased 45Ca outflow, [Ca2+]i and insulin output from islets exposed to 16.7 mM glucose and extracellular Ca2+. Hydroxylamine 0-13 insulin Homo sapiens 50-57 8798725-1 1996 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) lyase is inactivated by diethyl pyrocarbonate (DEPC); activity can be fully restored by incubation with hydroxylamine. Hydroxylamine 145-158 3-hydroxy-3-methylglutaryl-CoA lyase Homo sapiens 0-46 8952228-7 1996 RESULTS: We report: 1) a correlation between the induction of each CYP gene family and the O2 yield; 2) support to an observation reported previously that the tumor promoting ability of CYP inducers is mainly mediated by the O2; and 3) the description of a method for nitroxide mediated O2 detection in vivo. Hydroxylamine 268-277 cytochrome P450 family 4 subfamily F member 3 Homo sapiens 67-70 8952228-7 1996 RESULTS: We report: 1) a correlation between the induction of each CYP gene family and the O2 yield; 2) support to an observation reported previously that the tumor promoting ability of CYP inducers is mainly mediated by the O2; and 3) the description of a method for nitroxide mediated O2 detection in vivo. Hydroxylamine 268-277 cytochrome P450 family 4 subfamily F member 3 Homo sapiens 186-189 9131488-10 1996 Differences in rates of production of hydroxylamine metabolites of the drugs by cytochrome P450 (CYP2C9), myeloperoxidase, and thyroid, roxidase, along with an inherited abnormality in detoxification of the hydroxylamines are critically important in determining individual differences in adverse reaction risk. Hydroxylamine 38-51 cytochrome P450 family 2 subfamily C member 9 Homo sapiens 97-103 9131488-10 1996 Differences in rates of production of hydroxylamine metabolites of the drugs by cytochrome P450 (CYP2C9), myeloperoxidase, and thyroid, roxidase, along with an inherited abnormality in detoxification of the hydroxylamines are critically important in determining individual differences in adverse reaction risk. Hydroxylamine 38-51 myeloperoxidase Homo sapiens 106-121 9131488-11 1996 Both NATs, particularly NAT1, also can further metabolize hydroxylamine metabolites to N-acetoxy derivatives. Hydroxylamine 58-71 N-acetyltransferase 1 Homo sapiens 24-28 8823182-1 1996 Thirty consecutive single cysteine substitution mutants in the amino acids Q225-I256 of bovine rhodopsin have been prepared and modified with a sulfhydryl specific nitroxide reagent. Hydroxylamine 164-173 rhodopsin Bos taurus 95-104 8823182-7 1996 Judging from nitroxide mobilities, the putative extension of helix E in the aqueous phase is more dynamic than that of helix F. Changes in the electron paramagnetic resonance characteristics of the spin-labeled rhodopsin upon photoactivation indicate that chromophore isomerization results in patterns of structural changes that can be interpreted in terms of movements of helices that extend into the aqueous loop regions. Hydroxylamine 13-22 rhodopsin Bos taurus 211-220 8718880-3 1996 Treatment of the dehalogenase with diethyl pyrocarbonate resulted in complete loss of catalytic activity (Kinact = 0.17 mM-1 min-1 at pH 6.5, 25 degrees C) that was fully regained by subsequent treatment with hydroxylamine. Hydroxylamine 209-222 CD59 molecule (CD59 blood group) Homo sapiens 125-130 8769138-5 1996 In addition, hydroxylamine-induced mobility shifts were also apparent for synaptotagmins II and III, suggesting that posttranslational palmitoylation via thioester bonds may be a general modification of all synaptotagmins. Hydroxylamine 13-26 synaptotagmin 2 Rattus norvegicus 74-99 8818276-2 1996 The Lac Z" gene was truncated by removal of a 490 bp fragment which encoded 163 N-terminal residues of beta-galactosidase and was connected to the IGF-I cDNA by a linker encoding hydroxylamine cleavage recognition sequence. Hydroxylamine 179-192 insulin like growth factor 1 Homo sapiens 147-152 8818276-5 1996 After cleavage of the fusion protein with hydroxylamine, the released IGF-I was purified to homogeneity by a cation exchange chromatography, refolding and reverse-phase high performance liquid chromatography (rp-HPLC). Hydroxylamine 42-55 insulin like growth factor 1 Homo sapiens 70-75 8766706-4 1996 The corresponding hydroxylamine, N-hydroxy-2-aminofluorene (N-OH-AF), reduced cytochrome b specifically through center N of the cytochrome bc1 complex. Hydroxylamine 18-31 mitochondrially encoded cytochrome b Homo sapiens 78-90 8812179-4 1996 A number of metal-independent synthetic SOD mimics, based on organic nitroxides, have been tried as therapeutic interventions. Hydroxylamine 69-79 superoxide dismutase 1 Homo sapiens 40-43 8660602-3 1996 The predominant species formed in blood when MetHb formers, such as potassium ferricyanide, hydroxylamine, sodium nitrite, and 4-dimethylaminophenol (DMAP), added at molar ratios ranging from 1:10 to 1:1 to hemoglobin, are the valency hybrid intermediates alpha3+beta2+ and alpha2+beta3+. Hydroxylamine 92-105 hemoglobin subunit gamma 2 Homo sapiens 45-50 8924207-6 1996 However, the nitroxide mobility changes upon indole binding to the spin-labeled protein derivative were somewhat different between the two thrombin species under study. Hydroxylamine 13-22 coagulation factor II, thrombin Bos taurus 139-147 8666796-7 1996 The ADP-ribosyl-protein bonds in auto-ADP-ribosylated rat RT6.2, auto-ADP-ribosylated mouse Rt6, and ADP-ribosylhistone synthesized by Rt6 were stable to HgCl2 and HCl, but labile to NH2OH, consistent with ADP ribosylarginine linkages. Hydroxylamine 183-188 ADP-ribosyltransferase 2a Mus musculus 135-138 8639646-3 1996 Two different methods of analysis have provided very similar values for the angles alpha 1 and beta 1 which uniquely define the orientation of the nitroxide axis frame relative to the membrane normal axis. Hydroxylamine 147-156 adrenoceptor alpha 1D Homo sapiens 83-101 18626954-0 1996 Optimization of the hydroxylamine cleavage of an expressed fusion protein to produce recombinant human insulin-like growth factor (IGF)-I. Hydroxylamine 20-33 insulin like growth factor 1 Homo sapiens 103-137 18626954-3 1996 In this study, hydroxylamine cleavage conditions were modified to minimize unwanted chemical heterogeneity that occurred during the cleavage of the fusion protein [Met(1)]-pGH(1-11)-Val-Asn-IGF-I (Long-IGF-I). Hydroxylamine 15-28 insulin like growth factor 1 Homo sapiens 190-195 8622987-3 1996 Random hydroxylamine mutagenesis of qacB was undertaken, selecting for variants that conferred increased resistance to divalent cations. Hydroxylamine 7-20 antiseptic efflux protein QacB Staphylococcus aureus 36-40 27405952-0 1996 Structural dependence of nitroxide spin labels and nitroxide spin adducts on their reducibility by ascorbate ion. Hydroxylamine 25-34 spindlin 1 Homo sapiens 35-39 27405952-0 1996 Structural dependence of nitroxide spin labels and nitroxide spin adducts on their reducibility by ascorbate ion. Hydroxylamine 51-60 spindlin 1 Homo sapiens 61-65 8558235-4 1996 In this work we show the following: (1) an H2S-producing enzyme, cystathionine beta-synthase (CBS), is highly expressed in the hippocampus; (2) CBS inhibitors hydroxylamine and amino-oxyacetate suppress the production of brain H2S; and (3) a CBS activator, S-adenosyl-L-methionine, enhances H2S production, indicating that CBS contributes to the production of endogenous H2S. Hydroxylamine 159-172 cystathionine beta-synthase Homo sapiens 65-92 8558235-4 1996 In this work we show the following: (1) an H2S-producing enzyme, cystathionine beta-synthase (CBS), is highly expressed in the hippocampus; (2) CBS inhibitors hydroxylamine and amino-oxyacetate suppress the production of brain H2S; and (3) a CBS activator, S-adenosyl-L-methionine, enhances H2S production, indicating that CBS contributes to the production of endogenous H2S. Hydroxylamine 159-172 cystathionine beta-synthase Homo sapiens 94-97 8558235-4 1996 In this work we show the following: (1) an H2S-producing enzyme, cystathionine beta-synthase (CBS), is highly expressed in the hippocampus; (2) CBS inhibitors hydroxylamine and amino-oxyacetate suppress the production of brain H2S; and (3) a CBS activator, S-adenosyl-L-methionine, enhances H2S production, indicating that CBS contributes to the production of endogenous H2S. Hydroxylamine 159-172 cystathionine beta-synthase Homo sapiens 144-147 8558235-4 1996 In this work we show the following: (1) an H2S-producing enzyme, cystathionine beta-synthase (CBS), is highly expressed in the hippocampus; (2) CBS inhibitors hydroxylamine and amino-oxyacetate suppress the production of brain H2S; and (3) a CBS activator, S-adenosyl-L-methionine, enhances H2S production, indicating that CBS contributes to the production of endogenous H2S. Hydroxylamine 159-172 cystathionine beta-synthase Homo sapiens 144-147 8558235-4 1996 In this work we show the following: (1) an H2S-producing enzyme, cystathionine beta-synthase (CBS), is highly expressed in the hippocampus; (2) CBS inhibitors hydroxylamine and amino-oxyacetate suppress the production of brain H2S; and (3) a CBS activator, S-adenosyl-L-methionine, enhances H2S production, indicating that CBS contributes to the production of endogenous H2S. Hydroxylamine 159-172 cystathionine beta-synthase Homo sapiens 144-147 7578089-1 1995 Six nitroxide spin-labeled psoralen derivative have been synthesized and evaluated as probes for structural and dynamic studies. Hydroxylamine 4-13 spindlin 1 Homo sapiens 14-18 8573155-5 1996 Furthermore, the same mild hydroxylamine treatment caused partial ASGP-R inactivation; 50-70% of receptors corresponding to the previously characterized State 2 ASGP-Rs were inactivated. Hydroxylamine 27-40 mucin 4, cell surface associated Homo sapiens 66-70 8573155-5 1996 Furthermore, the same mild hydroxylamine treatment caused partial ASGP-R inactivation; 50-70% of receptors corresponding to the previously characterized State 2 ASGP-Rs were inactivated. Hydroxylamine 27-40 mucin 4, cell surface associated Homo sapiens 161-165 8725006-2 1996 The encoded protein contains VIP units separated by a linker peptide, potentially excisable by a double cleavage with endoprotease factor Xa and hydroxylamine. Hydroxylamine 145-158 vasoactive intestinal peptide Homo sapiens 29-32 9499162-0 1996 A beta (25-35) peptide displays H2O2-like reactivity towards aqueous Fe2+, nitroxide spin probes, and synaptosomal membrane proteins. Hydroxylamine 75-84 amyloid beta precursor protein Homo sapiens 0-6 8618850-4 1995 The [3H]palmitate labeling of GluR6 was eliminated by treatment with hydroxylamine, indicating that the labeling was due to palmitoylation at a cysteine residue via a thioester bond. Hydroxylamine 69-82 glutamate ionotropic receptor kainate type subunit 2 Homo sapiens 30-35 8719811-2 1995 In this study we investigated the role of catalase in relaxation induced by hydroxylamine, sodium azide, glyceryl trinitrate and hydrogen peroxide in isolated rings of rat aorta. Hydroxylamine 76-89 catalase Rattus norvegicus 42-50 8719811-14 1995 Pretreatment of endothelium-denuded rings with AT (1-50 mM, 90 min) to inhibit endogenous catalase blocked relaxation induced by sodium azide (1-300 nM) and hydroxylamine (1-300 nM) but had no effect on relaxation induced by hydrogen peroxide (10 microM-1 mM) or glyceryl trinitrate (1-100 nM). Hydroxylamine 157-170 catalase Rattus norvegicus 90-98 8719811-19 1995 These data suggest that metabolism by catalase plays an important role in the relaxation induced by hydroxylamine and sodium azide in isolated rings of rat aorta. Hydroxylamine 100-113 catalase Rattus norvegicus 38-46 8835945-5 1996 However hydroxylamine (1 M) can dissociate Dan-GB complexes in the presence of 0.1% SDS, both on membrane-bound and in free solution. Hydroxylamine 8-21 NBL1, DAN family BMP antagonist Homo sapiens 43-46 8597111-2 1995 The ESR signal of nitroxyl radical which was intravenously or intramuscularly injected to living female ddY mice decreased gradually by reducing to the corresponding hydroxylamine. Hydroxylamine 166-179 esterase 5 regulator Mus musculus 4-7 7578089-3 1995 Comparison of the general line shape features of the observed electron paramagnetic resonance (EPR) spectra of several duplexes ranging in size from 8 to 46 base pairs with simulated EPR spectra indicate that the nitroxide spin-label probe reports the global tumbling motion of the oligomers. Hydroxylamine 213-222 spindlin 1 Homo sapiens 223-227 7577919-2 1995 The paramagnetic effect from the nitroxide spin label, MTSSL, attached to cTnC(C35S) at Cys 84 allowed measurement of the relative distances to the 13C-methyl groups of the 10 methionines of cTnC in the monomer or complex. Hydroxylamine 33-42 troponin C1, slow skeletal and cardiac type Homo sapiens 74-78 7577919-2 1995 The paramagnetic effect from the nitroxide spin label, MTSSL, attached to cTnC(C35S) at Cys 84 allowed measurement of the relative distances to the 13C-methyl groups of the 10 methionines of cTnC in the monomer or complex. Hydroxylamine 33-42 troponin C1, slow skeletal and cardiac type Homo sapiens 191-195 7547976-4 1995 Fatty acid analysis demonstrated the labeled fatty acid incorporated into eNOS was palmitate, linked via a hydroxylamine-labile thioester bond. Hydroxylamine 107-120 nitric oxide synthase 3 Bos taurus 74-78 7545663-7 1995 A nitroxide spin-labeled version of the cNOS peptide caused broadening only for NMR resonances in the N-terminal half of CaM, showing that the peptide binds with a C to N orientation to the N- and C-terminal domains of CaM. Hydroxylamine 2-11 nitric oxide synthase 3 Rattus norvegicus 40-44 7545663-7 1995 A nitroxide spin-labeled version of the cNOS peptide caused broadening only for NMR resonances in the N-terminal half of CaM, showing that the peptide binds with a C to N orientation to the N- and C-terminal domains of CaM. Hydroxylamine 2-11 calmodulin 1 Rattus norvegicus 121-124 7545663-7 1995 A nitroxide spin-labeled version of the cNOS peptide caused broadening only for NMR resonances in the N-terminal half of CaM, showing that the peptide binds with a C to N orientation to the N- and C-terminal domains of CaM. Hydroxylamine 2-11 calmodulin 1 Rattus norvegicus 219-222 7540989-1 1995 The separations between aromatic residues in the bait region and nitroxide spin labels attached to the thiol ester-forming residues (Cys949 and Gln952) in human alpha 2-macroglobulin (alpha 2M) have been determined from paramagnetic broadening effects of the spin labels on bait region 1H NMR signals. Hydroxylamine 65-74 alpha-2-macroglobulin Homo sapiens 161-182 7586950-0 1995 Metabolism of dapsone to its hydroxylamine by CYP2E1 in vitro and in vivo. Hydroxylamine 29-42 cytochrome P450 family 2 subfamily E member 1 Homo sapiens 46-52 8529130-7 1995 Interaction of galectin-3 with AGE-BSA or FFI-BSA resulted in formation of SDS-, and beta-mercaptoethanol-insoluble, but hydroxylamine-sensitive high-molecular weight complexes between AGE-ligand, galectin-3, and other membrane components. Hydroxylamine 121-134 lectin, galactose binding, soluble 3 Mus musculus 15-25 7653521-6 1995 We also examined the DEPC effect on the transport activity of the triple histidine mutant (H35,120,349G) and found that NHE1 activity was still inhibited by DEPC with reversal by hydroxylamine and protected by amiloride and cimetidine. Hydroxylamine 179-192 solute carrier family 9 member A1 Homo sapiens 120-124 7631012-0 1995 Effect of gallium-porphyrin analogue ATX-70 on nitroxide formation from a cyclic secondary amine by ultrasound: on the mechanism of sonodynamic activation. Hydroxylamine 47-56 ectonucleotide pyrophosphatase/phosphodiesterase 2 Homo sapiens 37-40 7619803-7 1995 The photoinduced degradation of CP43 and the cross-linking among the D1 protein, CP43, and CP47 were less obvious in the PS II membranes that had been treated with hydroxylamine rather than Tris and in the membranes that had been treated with Tris and reconstituted by addition of an extrinsic 33-kDa protein (OEC33). Hydroxylamine 164-177 beaded filament structural protein 2 Homo sapiens 91-95 7619827-1 1995 In the presence of a suitable electron acceptor such as mammalian cytochrome c, hydroxylamine oxidoreductase (HAO) from the chemolithotrophic bacterium Nitrosomonas europaea catalyzes the oxidation of hydroxylamine or hydrazine to nitrite or dinitrogen, respectively. Hydroxylamine 80-93 cytochrome c, somatic Homo sapiens 66-78 7626044-2 1995 Here, we have used mutagenesis with hydroxylamine to generate a random library of PARP mutants. Hydroxylamine 36-49 poly(ADP-ribose) polymerase 1 Homo sapiens 82-86 7612622-1 1995 All 20 single cysteine substitution mutants in the sequence Y136-M155 of bovine rhodopsin have been prepared and modified with a sulfhydryl-specific nitroxide reagent. Hydroxylamine 149-158 rhodopsin Bos taurus 80-89 7540989-1 1995 The separations between aromatic residues in the bait region and nitroxide spin labels attached to the thiol ester-forming residues (Cys949 and Gln952) in human alpha 2-macroglobulin (alpha 2M) have been determined from paramagnetic broadening effects of the spin labels on bait region 1H NMR signals. Hydroxylamine 65-74 alpha-2-macroglobulin Homo sapiens 184-192 7767940-14 1995 These observations suggest that the initial reduction of the nitro group can be catalyzed by NADPH cytochrome P450 reductase alone but cytochrome P450 is needed in the reduction of the hydroxylamine to the amine. Hydroxylamine 185-198 cytochrome p450 oxidoreductase Rattus norvegicus 115-124 7793976-2 1995 The electron spin resonance (ESR) spectrum of SSL bound to LDL indicated that the nitroxide moiety was relatively mobile. Hydroxylamine 82-91 spindlin 1 Homo sapiens 13-17 7793976-5 1995 During Cu(2+)-initiated oxidation of spin-labeled LDL, nitroxide labels located in a hydrophobic environment were predominantly degraded. Hydroxylamine 55-64 spindlin 1 Homo sapiens 37-41 7779784-2 1995 Over 65% of initial ATPase activity (115 mumol of Pi/(mg.h)) was preserved after complete reaction of the enzyme with the lysine reactive nitroxide spin-labeled TEMPO isothiocyanate (TITC). Hydroxylamine 138-147 dynein axonemal heavy chain 8 Homo sapiens 20-26 8592522-6 1995 Because the ADP ribosylation of FGF-2 is acid resistant but base and hydroxylamine sensitive, the linkage appears to be mediated through arginine. Hydroxylamine 69-82 fibroblast growth factor 2 Homo sapiens 32-37 7726558-8 1995 Chemical cleavage with hydroxylamine and cyanogen bromide also revealed a similar pattern for liganded and unliganded AhR. Hydroxylamine 23-36 aryl-hydrocarbon receptor Mus musculus 118-121 7673174-12 1995 270, 21388-21395), we show that the ligand binding activity of affinity-purified State 2 ASGP-Rs is decreased by treatment with hydroxylamine under mild conditions consistent with these ASGP-Rs being fatty acylated in vivo. Hydroxylamine 128-141 mucin 4, cell surface associated Rattus norvegicus 89-93 7673174-12 1995 270, 21388-21395), we show that the ligand binding activity of affinity-purified State 2 ASGP-Rs is decreased by treatment with hydroxylamine under mild conditions consistent with these ASGP-Rs being fatty acylated in vivo. Hydroxylamine 128-141 mucin 4, cell surface associated Rattus norvegicus 186-190 7673174-16 1995 Pretreatment of ASGP-Rs with hydroxylamine before SDS-polyacrylamide gel electrophoresis reduced the content of both fatty acids by 66-80%, indicating that most of these fatty acids are attached to cysteine residues via thioester linkages. Hydroxylamine 29-42 mucin 4, cell surface associated Rattus norvegicus 16-20 7673175-7 1995 Here we show that approximately 50% of purified rat ASGP-Rs are inactivated by treatment with hydroxylamine under mild conditions. Hydroxylamine 94-107 mucin 4, cell surface associated Rattus norvegicus 52-56 7673175-9 1995 Treatment of ASGP-Rs in solution with 0.0125-1.0 M NH2OH, pH 7.4, at 4 degrees C for 4 h resulted in a progressive loss of ASOR binding activity. Hydroxylamine 51-56 mucin 4, cell surface associated Rattus norvegicus 13-17 7673175-10 1995 ASGP-R inactivation with NH2OH occurred more readily at basic pH or at room temperature. Hydroxylamine 25-30 mucin 4, cell surface associated Rattus norvegicus 0-4 7673175-13 1995 The more sensitive population of ASGP-Rs (approximately 50%) was inactivated by treatment with 0.2 M NH2OH (4 degrees C, 4 h) or with 1.0 M NH2OH (4 degrees C, 1 h) without detectable peptide cleavage as assessed by SDS-polyacrylamide gel electrophoresis. Hydroxylamine 101-106 mucin 4, cell surface associated Rattus norvegicus 33-37 7673175-13 1995 The more sensitive population of ASGP-Rs (approximately 50%) was inactivated by treatment with 0.2 M NH2OH (4 degrees C, 4 h) or with 1.0 M NH2OH (4 degrees C, 1 h) without detectable peptide cleavage as assessed by SDS-polyacrylamide gel electrophoresis. Hydroxylamine 140-145 mucin 4, cell surface associated Rattus norvegicus 33-37 7673175-14 1995 State 1 ASGP-Rs, purified from chloroquine- or monensin-treated hepatocytes, showed significantly less sensitivity to NH2OH treatment (both in kinetics and dose dependence). Hydroxylamine 118-123 mucin 4, cell surface associated Rattus norvegicus 8-12 7673175-15 1995 Furthermore, under mild conditions NH2OH caused dissociation and inactivation of approximately 50% of the total ASGP-Rs (State 1 and State 2) that were prebound to ASOR-Sepharose, whereas the same treatment caused dissociation of only < 20% of State 1 ASGP-Rs from such preformed complexes. Hydroxylamine 35-40 mucin 4, cell surface associated Rattus norvegicus 112-116 7673175-15 1995 Furthermore, under mild conditions NH2OH caused dissociation and inactivation of approximately 50% of the total ASGP-Rs (State 1 and State 2) that were prebound to ASOR-Sepharose, whereas the same treatment caused dissociation of only < 20% of State 1 ASGP-Rs from such preformed complexes. Hydroxylamine 35-40 mucin 4, cell surface associated Rattus norvegicus 255-259 7673175-20 1995 These radiolabeled fatty acids are completely released from purified ASGP-Rs by mild NH2OH treatment. Hydroxylamine 85-90 mucin 4, cell surface associated Rattus norvegicus 69-73 7763307-6 1995 The terminal oxidation product of hydroxylamine and hydroxyurea was the myoglobin-nitric oxide complex, one showing similar spectral characteristics to the analogous haemoglobin-nitric oxide adduct found in our previous experiments. Hydroxylamine 34-47 myoglobin Homo sapiens 72-81 7600914-2 1995 Inhibition of N-acetyltransferase (NAT)-dependent acetylation of dapsone could increase peak plasma concentrations of dapsone and shift the biotransformation pathway to the P450-mediated formation of a toxic metabolite of dapsone, the hydroxylamine. Hydroxylamine 235-248 bromodomain containing 2 Homo sapiens 14-33 8581424-0 1995 Hydroxylamine analogs of 2,6-di-t-butylphenols: dual inhibitors of cyclooxygenase and 5-lipoxygenase or selective 5-lipoxygenase inhibitors. Hydroxylamine 0-13 arachidonate 5-lipoxygenase Homo sapiens 86-100 8581424-0 1995 Hydroxylamine analogs of 2,6-di-t-butylphenols: dual inhibitors of cyclooxygenase and 5-lipoxygenase or selective 5-lipoxygenase inhibitors. Hydroxylamine 0-13 arachidonate 5-lipoxygenase Homo sapiens 114-128 8581424-1 1995 The preparation of hydroxylamine analogs of 2,6-di-tert-butylphenols (DTBP) and the inhibition of cyclooxygenase (CO) and 5-lipoxygenase (5-LO) by these compounds is discussed. Hydroxylamine 19-32 arachidonate 5-lipoxygenase Homo sapiens 122-136 7600914-2 1995 Inhibition of N-acetyltransferase (NAT)-dependent acetylation of dapsone could increase peak plasma concentrations of dapsone and shift the biotransformation pathway to the P450-mediated formation of a toxic metabolite of dapsone, the hydroxylamine. Hydroxylamine 235-248 bromodomain containing 2 Homo sapiens 35-38 7891082-7 1995 In contrast, mGluR4, which is coupled to inhibition of adenylyl cyclase, was found to be labelled with [3H]palmitic acid, and the palmitate was quantitatively removed by treatment with 1 M hydroxylamine, suggesting attachment of the palmitate through a thioester bond. Hydroxylamine 189-202 glutamate receptor, ionotropic, AMPA4 (alpha 4) Mus musculus 13-19 7961863-7 1994 The C3b-peptide complex was sensitive to hydroxylamine as was C3b-IgG indicating that both were ester-linked. Hydroxylamine 41-54 complement C3 Homo sapiens 4-7 7757197-3 1995 In the present study, we applied in vivo ESR to evaluate antioxidant activity by monitoring the redox reaction of nitroxide radical and clearly found that the nitroxide is very susceptible to oxidative stress in vivo and quite useful to evaluate antioxidant activity non-invasively. Hydroxylamine 114-123 esterase 5 regulator Mus musculus 41-44 9101236-1 1995 In DMSO solution, anthralin and its C-10 monosubstituted derivatives reduce nitroxides to the corresponding hydroxylamine derivatives, which are not further transformed. Hydroxylamine 76-86 homeobox C10 Homo sapiens 36-40 9101236-1 1995 In DMSO solution, anthralin and its C-10 monosubstituted derivatives reduce nitroxides to the corresponding hydroxylamine derivatives, which are not further transformed. Hydroxylamine 108-121 homeobox C10 Homo sapiens 36-40 9101236-3 1995 It is faster in DMSO than in DMF, piperidine type of nitroxides are reduced faster than the pyrrolidine type, and the substitution on C-10 of anthralin has a significant influence on the reaction rate. Hydroxylamine 53-63 homeobox C10 Homo sapiens 134-138 7758201-4 1995 Inclusion of the nucleophilic acceptors, ethanolamine or hydroxylamine, to the incubation mixture with biocytin and biotinidase resulted in loss of avidin reactivity. Hydroxylamine 57-70 biotinidase Homo sapiens 116-127 7831320-9 1995 7-Methyl-GMP was released from the nsP1-guanylate complex by treatment with acid or acidic hydroxylamine. Hydroxylamine 91-104 SH2 domain containing 3A Homo sapiens 35-39 7851446-2 1995 We have characterized the high-temperature molten globule state of bovine alpha-lactalbumin by a combined use of photochemically induced dynamic nuclear polarization and nitroxide surface perturbation. Hydroxylamine 170-179 lactalbumin alpha Bos taurus 74-91 7533613-19 1995 Islet guanosine 3":5"-cyclic monophosphate (cyclic GMP) content was not influenced by 10 mML-arginine or tert-butylhydroperoxide at 3 or 300 micro M but was markedly increased (14 fold) by a high hydroxylamine concentration (300 micro M). Hydroxylamine 196-209 5'-nucleotidase, cytosolic II Homo sapiens 51-54 7806998-10 1995 Furthermore, the C3 fragments bound to the M161 Ag were detached by 1 M hydroxylamine, suggesting that a covalent ester linkage sustains M161 Ag-C3b interaction. Hydroxylamine 72-85 complement C3 Homo sapiens 145-148 7833830-2 1994 It reduced the rate of methemoglobin formation from oxyhemoglobin exposed to nitric oxide generated from the reaction of hydroxylamine with Complex I of catalase and it decreased the amount of nitrite formed in the reaction of oxygen with nitric oxide generated from sodium nitroprusside. Hydroxylamine 121-134 catalase Rattus norvegicus 153-161 7966143-8 1994 The 8-aminooctanoic acid spacer arm placed the nitroxide moieties near the active site, near regions of the autolysis loops which differentiates between human alpha- and gamma-thrombin. Hydroxylamine 47-56 coagulation factor II, thrombin Bos taurus 176-184 7883753-8 1994 Most of the C3b-membrane molecule complexes were cleaved by hydroxylamine, suggesting covalent binding via an ester bond. Hydroxylamine 60-73 complement C3 Homo sapiens 12-15 7605614-1 1994 To understand the contributions of binding of elastin to domains removed from the active site of neutrophil elastase, we isolated an elastin-derived peptide (EDP) fraction, which we have previously shown was tightly linked to neutrophil elastase after prolonged digestion of elastin but which can be released from the enzyme with hydroxylamine. Hydroxylamine 330-343 elastin Homo sapiens 133-140 7817767-1 1994 The Friedel-Crafts reaction of benzene with cis-4-cyclohexene-1,2-dicarboxylic anhydride (1) yields trans-5-phenyl-cis-2-benzoylcyclohexanecarboxylic acid (2), which gave cyclohexane-condensed pyridazinone (3) with hydrazine, and cis-4,5-tetramethylene-1,2-oxazin-8-one (4) with hydroxylamine. Hydroxylamine 279-292 suppressor of cytokine signaling 6 Homo sapiens 44-49 7817767-1 1994 The Friedel-Crafts reaction of benzene with cis-4-cyclohexene-1,2-dicarboxylic anhydride (1) yields trans-5-phenyl-cis-2-benzoylcyclohexanecarboxylic acid (2), which gave cyclohexane-condensed pyridazinone (3) with hydrazine, and cis-4,5-tetramethylene-1,2-oxazin-8-one (4) with hydroxylamine. Hydroxylamine 279-292 suppressor of cytokine signaling 2 Homo sapiens 115-120 7817767-1 1994 The Friedel-Crafts reaction of benzene with cis-4-cyclohexene-1,2-dicarboxylic anhydride (1) yields trans-5-phenyl-cis-2-benzoylcyclohexanecarboxylic acid (2), which gave cyclohexane-condensed pyridazinone (3) with hydrazine, and cis-4,5-tetramethylene-1,2-oxazin-8-one (4) with hydroxylamine. Hydroxylamine 279-292 suppressor of cytokine signaling 6 Homo sapiens 230-235 7605614-1 1994 To understand the contributions of binding of elastin to domains removed from the active site of neutrophil elastase, we isolated an elastin-derived peptide (EDP) fraction, which we have previously shown was tightly linked to neutrophil elastase after prolonged digestion of elastin but which can be released from the enzyme with hydroxylamine. Hydroxylamine 330-343 elastin Homo sapiens 133-140 7926270-5 1994 Insulin secretion in HIT-T15 cells was inhibited by SIN-1, SNAP and hydroxylamine and in RINm5F cells by hydroxylamine. Hydroxylamine 68-81 insulin Mesocricetus auratus 0-7 7981788-2 1994 The spin-labeled nitrosoureas, triazenes and their precursor nitroxides exhibited excellent SSA, whereas clinically used nitrosourea and triazene, which do not contain the nitroxide moiety, did not show any SSA. Hydroxylamine 61-71 spindlin 1 Homo sapiens 4-8 7981788-2 1994 The spin-labeled nitrosoureas, triazenes and their precursor nitroxides exhibited excellent SSA, whereas clinically used nitrosourea and triazene, which do not contain the nitroxide moiety, did not show any SSA. Hydroxylamine 61-70 spindlin 1 Homo sapiens 4-8 7981016-3 1994 The mean residence time of the hydroxylamine metabolite was 5.5 +/- 1.5 h. The renal clearance of sulphamethoxazole hydroxylamine was 4.39 +/- 0.91 l h-1. Hydroxylamine 31-44 H1.5 linker histone, cluster member Homo sapiens 150-153 7997172-2 1994 By hydroxylamine mutagenesis two types of argR mutants were isolated and mapped. Hydroxylamine 3-16 arginine repressor Escherichia coli 42-46 7956732-0 1994 Evidence that the biotransformation of dapsone and monoacetyldapsone to their respective hydroxylamine metabolites in rat liver microsomes is mediated by cytochrome P450 2C6/2C11 and 3A1. Hydroxylamine 89-102 cytochrome P450, family 2, subfamily C, polypeptide 6, variant 1 Rattus norvegicus 154-173 7926270-5 1994 Insulin secretion in HIT-T15 cells was inhibited by SIN-1, SNAP and hydroxylamine and in RINm5F cells by hydroxylamine. Hydroxylamine 105-118 insulin Mesocricetus auratus 0-7 7764847-3 1994 The recombinant plasmid, pYHCgag3, gag gene was fused to the trpE" gene linked to the hydroxylamine (HA) cleavage recognition sequence which was induced to overexpress a core antigen (gag a.a. 121-398 from plasmid BH10) as fusion protein in the form of insoluble inclusion body. Hydroxylamine 101-103 Pr55(Gag) Human immunodeficiency virus 1 35-38 8135537-1 1994 Chemical modification with diethyl pyrocarbonate (DEPC) of the recombinant human liver UDP-glucuronosyltransferase UGT1*6 in enriched membrane fractions from a V79 cell line resulted in a rapid inactivation of the glucuronidation reaction, measured with 4-methyl-umbelliferone as aglycone substrate, with a second-order rate constant of 3110 M-1.min-1 at pH 6.0 and 25 degrees C. The enzymatic activity was restored by hydroxylamine. Hydroxylamine 419-432 UDP glucuronosyltransferase family 1 member A6 Homo sapiens 115-121 8107883-5 1994 We find that some S-nitrosothiols, dinitrosyl-iron-cysteine complex, sodium nitroxyl and hydroxylamine can be eliminated as candidates as they are more stable than EDRF and less susceptible to inhibition by oxyhaemoglobin. Hydroxylamine 89-102 alpha hemoglobin stabilizing protein Homo sapiens 164-168 8110774-2 1994 Approximately 70% of the rhodopsin was depalmitoylated in rod outer segments by a mild hydroxylamine treatment that resulted in minimal bleaching of rhodopsin. Hydroxylamine 87-100 rhodopsin Homo sapiens 25-34 8125130-5 1994 Hydroxylamine treatment of the 14C-C3frg-Fab and 14C-C3frg-Fc complexes released a 14C-C3frg of similar size (about 3-4 kDa) in which the N-terminal residue was the radiolabeled Cys1010. Hydroxylamine 0-13 FA complementation group B Homo sapiens 41-44 8121410-2 1994 Random mutations were induced in vitro in the HSP82 gene by treatment of the plasmid with hydroxylamine. Hydroxylamine 90-103 Hsp90 family chaperone HSP82 Saccharomyces cerevisiae S288C 46-51 8110774-2 1994 Approximately 70% of the rhodopsin was depalmitoylated in rod outer segments by a mild hydroxylamine treatment that resulted in minimal bleaching of rhodopsin. Hydroxylamine 87-100 rhodopsin Homo sapiens 149-158 8110774-3 1994 Subsequent purification by affinity chromatography could be used to remove hydroxylamine-bleached rhodopsin. Hydroxylamine 75-88 rhodopsin Homo sapiens 98-107 8208058-5 1994 Blood nitroxide levels also declined exponentially with time with an initial t1/2 of 70 minutes and a terminal t1/2 of 10 hours. Hydroxylamine 6-15 brachyury, T-box transcription factor T Mus musculus 77-87 8821708-5 1994 We found the inhibition of ADP-ribosylation of MBP by hydroxylamine and L-arginine indicating that this modification was likely to be mediated by arginine residues. Hydroxylamine 54-67 myelin basic protein Gallus gallus 47-50 8208058-5 1994 Blood nitroxide levels also declined exponentially with time with an initial t1/2 of 70 minutes and a terminal t1/2 of 10 hours. Hydroxylamine 6-15 brachyury, T-box transcription factor T Mus musculus 111-121 8344932-5 1993 The putative human leukocyte elastase-elastin-derived peptide complex was treated with hydroxylamine to cleave any possible acyl-enzyme complexes and was then measured for amidolytic activity. Hydroxylamine 87-100 elastin Homo sapiens 38-45 8227060-15 1993 Digestion of the E-GMP with hydroxylamine at pH 9.5 yielded a 31-kDa radioactive fragment derived from amino acids 1-273. Hydroxylamine 28-41 5'-nucleotidase, cytosolic II Homo sapiens 19-22 8228253-9 1993 [3H]AA release and MAC-1 expression in MLD and SLD-treated hPMNL could be recovered in the presence of 1 mM hydroxylamine in a time-dependent fashion, consistent with reported data that MLD and SLD inactivate PLA2 through Schiff base formation. Hydroxylamine 108-121 integrin subunit alpha M Homo sapiens 19-24 8228253-9 1993 [3H]AA release and MAC-1 expression in MLD and SLD-treated hPMNL could be recovered in the presence of 1 mM hydroxylamine in a time-dependent fashion, consistent with reported data that MLD and SLD inactivate PLA2 through Schiff base formation. Hydroxylamine 108-121 phospholipase A2 group IB Homo sapiens 209-213 8226820-6 1993 At high micromolar concentrations, the organic nitroxides 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO) and 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPOL) were inhibitory in the cytochrome c assay, but showed no detectable SOD activity by stopped-flow. Hydroxylamine 47-57 cytochrome c, somatic Homo sapiens 186-198 8226820-6 1993 At high micromolar concentrations, the organic nitroxides 2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPO) and 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-yloxy (TEMPOL) were inhibitory in the cytochrome c assay, but showed no detectable SOD activity by stopped-flow. Hydroxylamine 47-57 superoxide dismutase 1 Homo sapiens 231-234 8239061-3 1993 Blockade of the hydroxylamine vasodilator response by methylene blue indicated that the mechanism of vasorelaxation is similar to that of EDRF. Hydroxylamine 16-29 alpha hemoglobin stabilizing protein Homo sapiens 138-142 8240371-3 1993 We have isolated 159 hydroxylamine-induced nrdB mutants, determined which mutations are in nrdB by marker rescue with clones of the nrdB gene and have mapped these mutations by marker rescue using subclones of the nrdB intron. Hydroxylamine 21-34 ribonucleotide-diphosphate reductase subunit beta Escherichia phage T4 43-47 8240371-3 1993 We have isolated 159 hydroxylamine-induced nrdB mutants, determined which mutations are in nrdB by marker rescue with clones of the nrdB gene and have mapped these mutations by marker rescue using subclones of the nrdB intron. Hydroxylamine 21-34 ribonucleotide-diphosphate reductase subunit beta Escherichia phage T4 91-95 8240371-3 1993 We have isolated 159 hydroxylamine-induced nrdB mutants, determined which mutations are in nrdB by marker rescue with clones of the nrdB gene and have mapped these mutations by marker rescue using subclones of the nrdB intron. Hydroxylamine 21-34 ribonucleotide-diphosphate reductase subunit beta Escherichia phage T4 91-95 8240371-3 1993 We have isolated 159 hydroxylamine-induced nrdB mutants, determined which mutations are in nrdB by marker rescue with clones of the nrdB gene and have mapped these mutations by marker rescue using subclones of the nrdB intron. Hydroxylamine 21-34 ribonucleotide-diphosphate reductase subunit beta Escherichia phage T4 91-95 8394810-6 1993 One mutant (LTN1), arising from hydroxylamine mutagenesis, has been studied in detail: Assembly of the enzyme appears to be normal, as judged from the levels of the subunits observed in Western blots, while spectral analysis showed that only holo-cytochrome b was lowered to 70% of that of the wildtype. Hydroxylamine 32-45 ubiquitin-protein ligase RKR1 Saccharomyces cerevisiae S288C 12-16 8341280-4 1993 C3b was covalently bound to Superose through an ester link, as indicated by lability to hydroxylamine treatment at alkaline pH. Hydroxylamine 88-101 complement C3 Homo sapiens 0-3 8383676-5 1993 Analysis of the hydroxylamine-dependent cleavage of ubiquitin-Cdc34 conjugates at the single Asn-Gly sequence in Cdc34 placed the major ubiquitin linkage site within the C-terminal 215-295 residues of Cdc34. Hydroxylamine 16-29 ubiquitin Saccharomyces cerevisiae S288C 52-61 8386638-6 1993 Prior to analysis of the phosphorylation mixture, the phosphorylated form of photoexcited rhodopsin was converted into phospho-opsin by treatment with NH2OH. Hydroxylamine 151-156 rhodopsin Homo sapiens 90-99 8484722-3 1993 Like actin, the ADP-ribose-arthrin linkage was sensitive towards hydroxylamine treatment, indicating arginine as the amino acid acceptor. Hydroxylamine 65-78 Actin 79B Drosophila melanogaster 5-10 8466547-10 1993 However, in the hepatocyte NAT2 variation may be an important competitive pathway which influences the extent of oxidative metabolism of SMX to its reactive hydroxylamine metabolite. Hydroxylamine 157-170 N-acetyltransferase 2 Homo sapiens 27-31 8383676-5 1993 Analysis of the hydroxylamine-dependent cleavage of ubiquitin-Cdc34 conjugates at the single Asn-Gly sequence in Cdc34 placed the major ubiquitin linkage site within the C-terminal 215-295 residues of Cdc34. Hydroxylamine 16-29 SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 Saccharomyces cerevisiae S288C 62-67 8383676-5 1993 Analysis of the hydroxylamine-dependent cleavage of ubiquitin-Cdc34 conjugates at the single Asn-Gly sequence in Cdc34 placed the major ubiquitin linkage site within the C-terminal 215-295 residues of Cdc34. Hydroxylamine 16-29 SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 Saccharomyces cerevisiae S288C 113-118 8383676-5 1993 Analysis of the hydroxylamine-dependent cleavage of ubiquitin-Cdc34 conjugates at the single Asn-Gly sequence in Cdc34 placed the major ubiquitin linkage site within the C-terminal 215-295 residues of Cdc34. Hydroxylamine 16-29 ubiquitin Saccharomyces cerevisiae S288C 136-145 8383676-5 1993 Analysis of the hydroxylamine-dependent cleavage of ubiquitin-Cdc34 conjugates at the single Asn-Gly sequence in Cdc34 placed the major ubiquitin linkage site within the C-terminal 215-295 residues of Cdc34. Hydroxylamine 16-29 SCF E2 ubiquitin-protein ligase catalytic subunit CDC34 Saccharomyces cerevisiae S288C 113-118 8282226-5 1993 However, with the use of a nitroxide contrast agent (3-PCA) there was no enhancement, but rather a significant diminution of the CCl4-induced edematous response. Hydroxylamine 27-36 C-C motif chemokine ligand 4 Rattus norvegicus 129-133 8464354-3 1993 Hydroxylamine treatment of vimentin, separated by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE), failed to remove either palmitate or myristate derived radiolabel. Hydroxylamine 0-13 vimentin Bos taurus 27-35 8282226-6 1993 These results suggest that the nitroxide contrast agents, which are themselves free radicals, act as free radical scavengers and therefore reduce the formation of the CCl4-induced hepatic "damage" observed in proton MR images. Hydroxylamine 31-40 C-C motif chemokine ligand 4 Rattus norvegicus 167-171 8477265-0 1993 Detection of point mutations in the p53 gene: comparison of single-strand conformation polymorphism, constant denaturant gel electrophoresis, and hydroxylamine and osmium tetroxide techniques. Hydroxylamine 146-159 tumor protein p53 Homo sapiens 36-39 8396553-3 1993 During EPR studies of chemical, biochemical, and cellular processes involving free radicals, molecular oxygen has significant magnetic influence on the EPR signal intensity of the free radical species under investigation in addition to affecting the rates of production of the primary species and the stability of the spin adduct nitroxides. Hydroxylamine 330-340 spindlin 1 Homo sapiens 318-322 8396553-5 1993 In the present study, the effects of oxygen and ferricyanide on the EPR signal height of stable and persistent spin adduct nitroxides at commonly employed microwave powers were examined. Hydroxylamine 123-133 spindlin 1 Homo sapiens 111-115 1406583-3 1992 Using a plasmid shuffle screen, we isolated mutations in the HSF1 gene after in vitro mutagenesis of plasmid DNA with hydroxylamine. Hydroxylamine 118-131 stress-responsive transcription factor HSF1 Saccharomyces cerevisiae S288C 61-65 1333213-5 1992 In general, the nitroxide immobilization was greater for spin-labeled thrombin complexes with HV2-Lys 47 vs. HV1. Hydroxylamine 16-25 coagulation factor II, thrombin Homo sapiens 70-78 1333213-5 1992 In general, the nitroxide immobilization was greater for spin-labeled thrombin complexes with HV2-Lys 47 vs. HV1. Hydroxylamine 16-25 hydrogen voltage gated channel 1 Homo sapiens 109-112 1360444-5 1992 We then found that virtually all Thy-1 molecules on thymocytes became sensitive to PI-PLC when they were treated with hydroxylamine that should cleave ester-linked lipids. Hydroxylamine 118-131 thymus cell antigen 1, theta Mus musculus 33-38 1360444-5 1992 We then found that virtually all Thy-1 molecules on thymocytes became sensitive to PI-PLC when they were treated with hydroxylamine that should cleave ester-linked lipids. Hydroxylamine 118-131 protein disulfide isomerase associated 3 Mus musculus 83-89 1431805-3 1992 Rhodopsin was inactivated by exposure to hydroxylamine and bright light. Hydroxylamine 41-54 rhodopsin Homo sapiens 0-9 1281490-4 1992 In addition, we show that SNAP-25/SuP is the most prominent species among retinal polypeptides that incorporate 3H-palmitate in vivo, that it is fatty acylated through a hydroxylamine-labile, thioester bond, and that palmitoylated SNAP-25/SuP is axonally transported. Hydroxylamine 170-183 synaptosome associated protein 25 Rattus norvegicus 26-33 1281490-4 1992 In addition, we show that SNAP-25/SuP is the most prominent species among retinal polypeptides that incorporate 3H-palmitate in vivo, that it is fatty acylated through a hydroxylamine-labile, thioester bond, and that palmitoylated SNAP-25/SuP is axonally transported. Hydroxylamine 170-183 synaptosome associated protein 25 Rattus norvegicus 231-238 1329957-7 1992 However, being more polar, the nitroxide spin probe CSL is preferentially located near the surface of the membrane, while the less polar fluorescent probe DPH reports preferentially near the central hydrophobic region of the lipid bilayers. Hydroxylamine 31-40 chorionic somatomammotropin hormone like 1 Homo sapiens 52-55 1429836-6 1992 When VPS1 was treated with hydroxylamine, half of all mutations isolated were found to be dominant negative with respect to vacuolar protein sorting. Hydroxylamine 27-40 dynamin-like GTPase VPS1 Saccharomyces cerevisiae S288C 5-9 1518857-6 1992 By hydroxylamine mutagenesis, we have identified a rad1 mutant allele whose encoded protein fails to complex with RAD10. Hydroxylamine 3-16 ssDNA endodeoxyribonuclease RAD1 Saccharomyces cerevisiae S288C 51-55 1324124-6 1992 Maximum nitroxide formation was observed after 90 min; the addition of SOD (93 units/ml) increased the concentration of nitroxide. Hydroxylamine 8-17 superoxide dismutase 1 Homo sapiens 71-74 1324124-6 1992 Maximum nitroxide formation was observed after 90 min; the addition of SOD (93 units/ml) increased the concentration of nitroxide. Hydroxylamine 120-129 superoxide dismutase 1 Homo sapiens 71-74 1330032-1 1992 Permeation of molecular oxygen in rhodopsin, an integral membrane protein, has been investigated by monitoring the bimolecular collision rate between molecular oxygen and the nitroxide spin label using a pulse electron spin resonance (ESR) T1 method. Hydroxylamine 175-184 rhodopsin Homo sapiens 34-43 1634535-6 1992 Subsequent mild treatment of the covalent thrombin-inhibitor complexes with NH2OH in the presence of 5-(iodoacetamido)fluorescein resulted in generation of the thiol group together with its selective modification and incorporation of 0.96 +/- 0.07 mol of probe/mol of active sites. Hydroxylamine 76-81 coagulation factor II, thrombin Homo sapiens 42-50 1634536-4 1992 An array of 16 thrombin derivatives was prepared by affinity labeling of the proteinase active site with the thioester peptide chloromethyl ketones, N alpha-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CH2Cl or N alpha-[(acetylthio)acetyl]-D-Phe-Phe-Arg-CH2Cl, followed by selective modification of the NH2OH-generated thiol group on the covalently incorporated inhibitors with each of eight thiol-reactive fluorescence probes. Hydroxylamine 293-298 coagulation factor II, thrombin Homo sapiens 15-23 1634536-4 1992 An array of 16 thrombin derivatives was prepared by affinity labeling of the proteinase active site with the thioester peptide chloromethyl ketones, N alpha-[(acetylthio)acetyl]-D-Phe-Pro-Arg-CH2Cl or N alpha-[(acetylthio)acetyl]-D-Phe-Phe-Arg-CH2Cl, followed by selective modification of the NH2OH-generated thiol group on the covalently incorporated inhibitors with each of eight thiol-reactive fluorescence probes. Hydroxylamine 293-298 endogenous retrovirus group K member 18 Homo sapiens 77-87 1637301-0 1992 Chemical heterogeneity as a result of hydroxylamine cleavage of a fusion protein of human insulin-like growth factor I. Hydroxylamine 38-51 insulin like growth factor 1 Homo sapiens 90-118 1318696-2 1992 The purified enzyme was spin labeled by a nitroxide derivative of p-chloromercuribenzoate. Hydroxylamine 42-51 spindlin 1 Rattus norvegicus 24-28 1637301-3 1992 In order to release mature recombinant-derived hIGF-I (rhIGF-I), the fusion protein is treated with hydroxylamine, which cleaves a susceptible Asn-Gly bond that has been engineered into the fusion protein gene. Hydroxylamine 100-113 insulin like growth factor 1 Homo sapiens 47-53 1315759-0 1992 Nitroxide metabolites from alkylhydroxylamines and N-hydroxyurea derivatives resulting from reductive inhibition of soybean lipoxygenase. Hydroxylamine 0-9 linoleate 9S-lipoxygenase-4 Glycine max 124-136 1321158-4 1992 The NH2-terminal domain of GAD65 is the site of a two-step modification, the last of which results in a firm membrane anchoring that involves posttranslational hydroxylamine sensitive palmitoylation. Hydroxylamine 160-173 glutamate decarboxylase 2 Homo sapiens 27-32 1314651-2 1992 The unusual reaction of sct-PA afforded the opportunity to directly compare the active site environment of sct-PA and two-chain tissue plasminogen activator (tct-PA) in solution through the application of a series of nitroxide spin labels and fluorophores. Hydroxylamine 217-226 chromosome 20 open reading frame 181 Homo sapiens 128-156 1423015-6 1992 There was no increase in TNF-alpha production seen during incubation with sulphonamide reactive metabolites; rather, there was a decrease in TNF-alpha elaboration that was most marked when PBMCs were incubated with the hydroxylamine of sulfamethoxazole. Hydroxylamine 219-232 tumor necrosis factor Mus musculus 141-150 1551104-2 1992 The stable nitroxide 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (Tempol) has recently been shown to protect aerated cells in culture against superoxide generated from hypoxanthine/xanthine oxidase, hydrogen peroxide, and radiation-induced cytotoxicity and to modestly sensitive hypoxic cultured cells. Hydroxylamine 11-20 xanthine dehydrogenase Mus musculus 184-200 1740436-8 1992 (a) Inactivation of chitinase with EDC is reversible by treatment with hydroxylamine. Hydroxylamine 71-84 chitinase chem 5 Zea mays 20-29 1600153-3 1992 In this study, the chemical probes osmium tetroxide, diethylpyrocarbonate and hydroxylamine were used to show that a tract of alternating purines and pyrimidines in the Adh1 promoter (from -311 to -325) actually assumes a Z-DNA conformation under superhelical stress in vitro. Hydroxylamine 78-91 alcohol dehydrogenase 1 Zea mays 169-173 1387847-0 1992 Hydroxylamine-dependent inhibition of rhodopsin phosphorylation in the isolated retina. Hydroxylamine 0-13 rhodopsin Bos taurus 38-47 1387847-1 1992 Hydroxylamine (NH2OH), a substance known to accelerate the decay of the metarhodopsin II bleaching intermediate of rhodopsin, was examined for its effect on the light-dependent phosphorylation of rhodopsin in the intact, isolated retina. Hydroxylamine 0-13 rhodopsin Bos taurus 76-85 1387847-1 1992 Hydroxylamine (NH2OH), a substance known to accelerate the decay of the metarhodopsin II bleaching intermediate of rhodopsin, was examined for its effect on the light-dependent phosphorylation of rhodopsin in the intact, isolated retina. Hydroxylamine 0-13 rhodopsin Bos taurus 115-124 1387847-1 1992 Hydroxylamine (NH2OH), a substance known to accelerate the decay of the metarhodopsin II bleaching intermediate of rhodopsin, was examined for its effect on the light-dependent phosphorylation of rhodopsin in the intact, isolated retina. Hydroxylamine 15-20 rhodopsin Bos taurus 76-85 1387847-1 1992 Hydroxylamine (NH2OH), a substance known to accelerate the decay of the metarhodopsin II bleaching intermediate of rhodopsin, was examined for its effect on the light-dependent phosphorylation of rhodopsin in the intact, isolated retina. Hydroxylamine 15-20 rhodopsin Bos taurus 115-124 1387847-7 1992 The results indicate that the light-dependent phosphorylation of rhodopsin in situ is substantially inhibited by NH2OH at millimolar levels. Hydroxylamine 113-118 rhodopsin Bos taurus 65-74 1569385-2 1992 Cleavage of heteroduplexes formed between normal and patient DNA strands with hydroxylamine and osmium tetroxide readily localized a mutation near base 2660 of the mutant apoC-II. Hydroxylamine 78-91 apolipoprotein C2 Homo sapiens 171-178 1311930-3 1992 The latter two residues provide a unique hydroxylamine-sensitive link between [Met1]-pGH(1-46) and the N-terminal Gly of IGF-I. Hydroxylamine 41-54 IGFI Bos taurus 121-126 1662795-1 1991 Using the hydroxylamine-osmium tetroxide (HOT) technique, we have identified a constitutional point mutation in the retinoblastoma susceptibility gene (RB1) which segregates with the expression of retinoblastoma in five affected family members. Hydroxylamine 10-23 RB transcriptional corepressor 1 Homo sapiens 152-155 1662085-2 1991 This apparatus was previously employed to measure spin label pharmacokinetics of nitroxide sensitive to oxygen in whole mice. Hydroxylamine 81-90 spindlin 1 Mus musculus 50-54 1315759-5 1992 It is consistently found that the selected NOH-containing compounds, e.g. alkylhydroxylamines or N-hydroxyureas, are also oxidized by lipoxygenase to form their corresponding nitroxides. Hydroxylamine 175-185 linoleate 9S-lipoxygenase-4 Glycine max 134-146 1530946-14 1992 In vivo labeling studies confirmed that myristate is covalently attached to ISA via a hydroxylamine resistant amide linkage. Hydroxylamine 86-99 annexin A13 Homo sapiens 76-79 1317587-1 1992 The ability of spermatozoa to reduce nitroxide spin--TEMPO has been used as a parameter to understand maturation, capacitation and calcium uptake of sperm obtained from Holstman strain rats. Hydroxylamine 37-46 spindlin 1 Rattus norvegicus 47-51 1947794-4 1991 The linkage was localized between the alpha" chain of C3b and either the H or L chain of TT; it required the in situ formation of C3b and was partially destroyed by 1 M hydroxylamine. Hydroxylamine 169-182 complement C3 Homo sapiens 54-57 1947794-4 1991 The linkage was localized between the alpha" chain of C3b and either the H or L chain of TT; it required the in situ formation of C3b and was partially destroyed by 1 M hydroxylamine. Hydroxylamine 169-182 complement C3 Homo sapiens 130-133 1654082-1 1991 We prepared, purified, and characterized derivatives of epidermal growth factor (EGF) having a nitroxide spin-label attached covalently at the amino terminus. Hydroxylamine 95-104 epidermal growth factor Homo sapiens 56-79 1939072-0 1991 Depalmitylation with hydroxylamine alters the functional properties of rhodopsin. Hydroxylamine 21-34 rhodopsin Rattus norvegicus 71-80 1939072-2 1991 Using hydroxylamine (NH2OH) to cleave the thioester linkage, we have characterized the effect of depalmitylation on certain functional properties of rhodopsin. Hydroxylamine 6-19 rhodopsin Rattus norvegicus 149-158 1939072-2 1991 Using hydroxylamine (NH2OH) to cleave the thioester linkage, we have characterized the effect of depalmitylation on certain functional properties of rhodopsin. Hydroxylamine 21-26 rhodopsin Rattus norvegicus 149-158 1939072-3 1991 Treatment of rod outer segment membranes (prepared from rat retinas previously labeled in vivo with [3H]palmitate) with 1 M NH2OH typically removed greater than or equal to 75% of the [3H]palmitate initially bound to rhodopsin. Hydroxylamine 124-129 rhodopsin Rattus norvegicus 217-226 1939072-4 1991 Spectrophotometry of rod outer segment membranes that had been treated with 1 M NH2OH indicated preservation of 85% of the native rhodopsin and no effect on the shape of the absorbance spectrum of rhodopsin. Hydroxylamine 80-85 rhodopsin Rattus norvegicus 130-139 1939072-5 1991 In vivo labeled rhodopsin that had been treated with 1 M NH2OH did not reincorporate free endogenous [3H] palmitate over a 2-h incubation period. Hydroxylamine 57-62 rhodopsin Rattus norvegicus 16-25 1939072-6 1991 Both NH2OH-treated and untreated rhodopsin incorporated [14C]palmitate from exogenously added [14C]palmitoyl-CoA. Hydroxylamine 5-10 rhodopsin Rattus norvegicus 33-42 1939072-8 1991 The removal of palmitate by NH2OH inhibited rhodopsin regeneration by 44% and increased the ability of rhodopsin to activate transducin"s light-dependent GTPase activity by 61%. Hydroxylamine 28-33 rhodopsin Rattus norvegicus 44-53 1654082-1 1991 We prepared, purified, and characterized derivatives of epidermal growth factor (EGF) having a nitroxide spin-label attached covalently at the amino terminus. Hydroxylamine 95-104 epidermal growth factor Homo sapiens 81-84 1648392-4 1991 Diethyl pyrocarbonate (DEP), a histidine-modifying agent, inhibits CaM-PDE with a second-order rate constant of 130 M-1 min-1 at pH 7.0 and 30 degrees C. Activity is restored by NH2OH. Hydroxylamine 178-183 calmodulin Bos taurus 67-70 1646612-1 1991 Clearance of the nitroxide radicals, hydroxy-TEMPO and carboxy-PROXYL, in whole-mouse lung was directly measured by in vivo ESR. Hydroxylamine 17-26 esterase 5 regulator Mus musculus 124-127 1799700-1 1991 Hydroxylamine-induced mutations in the virA gene of Agrobacterium tumefaciens that do not require the plant phenolic-inducing compound acetosyringone for vir regulon induction were isolated. Hydroxylamine 0-13 two-component VirA-like sensor kinase Agrobacterium tumefaciens 39-43 1645351-4 1991 We isolated 18 unique hydroxylamine-induced missense mutations in the LAR domain I segment, of which three were temperature-sensitive. Hydroxylamine 22-35 protein tyrosine phosphatase receptor type F Homo sapiens 70-73 2038186-2 1991 We investigated the ability of a stable nitroxide spin trap, TEMPOL, to protect TNF-sensitive cells from exogenously added TNF. Hydroxylamine 40-49 tumor necrosis factor Mus musculus 80-83 2065728-7 1991 Treatment of purified rhodopsin with 1 M hydroxylamine released similar amounts of palmitate from the rd mice and controls. Hydroxylamine 41-54 rhodopsin Mus musculus 22-31 2038186-2 1991 We investigated the ability of a stable nitroxide spin trap, TEMPOL, to protect TNF-sensitive cells from exogenously added TNF. Hydroxylamine 40-49 tumor necrosis factor Mus musculus 123-126 1671745-2 1991 It has been proposed that NH2OH is a putative intermediate in the biochemical pathway for the generation of nitric oxide (NO)/endothelium-derived relaxing factor (EDRF) from L-arginine. Hydroxylamine 26-31 alpha hemoglobin stabilizing protein Mus musculus 163-167 1671745-3 1991 NH2OH caused a time- and concentration-dependent increase in cyclic GMP formation in intact cells. Hydroxylamine 0-5 5'-nucleotidase, cytosolic II Mus musculus 68-71 1671745-7 1991 The formation of cyclic GMP in the cytosolic fractions in response to NH2OH required the addition of catalase and H2O2. Hydroxylamine 70-75 5'-nucleotidase, cytosolic II Mus musculus 24-27 1671745-7 1991 The formation of cyclic GMP in the cytosolic fractions in response to NH2OH required the addition of catalase and H2O2. Hydroxylamine 70-75 catalase Mus musculus 101-109 1671745-9 1991 Furthermore, NH2OH inhibited L-arginine- and sodium nitroprusside-induced cyclic GMP formation in the cytosol. Hydroxylamine 13-18 5'-nucleotidase, cytosolic II Mus musculus 81-84 1671745-10 1991 The inhibition of L-arginine-induced cyclic GMP formation in the cytosol by NH2OH was not reversed by the addition of superoxide dismutase. Hydroxylamine 76-81 5'-nucleotidase, cytosolic II Mus musculus 44-47 1849455-12 1991 Amphiphilic dimers of AChE from Drosophila, from mouse erythrocytes, and from the human erythroleukaemia cell line K562 also resist the treatment with hydroxylamine and likely possess a terminal alkylacylglycerol. Hydroxylamine 151-164 Acetylcholine esterase Drosophila melanogaster 22-26 1850875-3 1991 Overall, the nitroxide moieties were more immobilized in the bovine vs human derivatives reflecting either more apolar binding regions or steric obstructions to the motion of the nitroxide in bovine thrombin. Hydroxylamine 13-22 coagulation factor II, thrombin Bos taurus 199-207 1850875-3 1991 Overall, the nitroxide moieties were more immobilized in the bovine vs human derivatives reflecting either more apolar binding regions or steric obstructions to the motion of the nitroxide in bovine thrombin. Hydroxylamine 179-188 coagulation factor II, thrombin Bos taurus 199-207 1850875-5 1991 The two active site directed fluorophores, dansyl fluoride and p-nitrophenyl anthranilate showed differences in both lambda emmax and lambda exmax of the complexes with bovine and human-alpha-thrombin, respectively, Several of the effects observed i.e., ligand binding (indole or benzamidine) and the subtle hydrophobic interactions between the nitroxide moiety and the protein active site would be difficult to assess from an x-ray structure determination alone. Hydroxylamine 345-354 coagulation factor II, thrombin Homo sapiens 192-200 15374470-7 1990 This statement has been proven by showing (i) that O2* radicals generated during the autoxidation of ID-H can directly be trapped on DMPO; (ii) the effect of ID-H on the OH* free radicals is abolished if SOD is added to the system; (iii) the O2(-*) radicals generated by ID-H autoxidation reduce directly the OH-spin adducts on various kinds of nitroxide type spin traps (e.g. TEMPO, TMPN). Hydroxylamine 345-354 isocitrate dehydrogenase (NADP(+)) 1 Homo sapiens 101-105 1664324-2 1991 The results can be interpreted in terms of a two dimensional translational diffusion of the nitroxide tips of the spin labels along the protein surface within restricted surface areas. Hydroxylamine 92-101 spindlin 1 Homo sapiens 114-118 1663907-3 1991 The hydroxylamine is oxidized to the radical t-BuN(O)H (2) which is converted into the spin trap 2-methyl-2-nitrosopropane (3). Hydroxylamine 4-17 spindlin 1 Homo sapiens 87-91 15374470-7 1990 This statement has been proven by showing (i) that O2* radicals generated during the autoxidation of ID-H can directly be trapped on DMPO; (ii) the effect of ID-H on the OH* free radicals is abolished if SOD is added to the system; (iii) the O2(-*) radicals generated by ID-H autoxidation reduce directly the OH-spin adducts on various kinds of nitroxide type spin traps (e.g. TEMPO, TMPN). Hydroxylamine 345-354 isocitrate dehydrogenase (NADP(+)) 1 Homo sapiens 158-162 15374470-7 1990 This statement has been proven by showing (i) that O2* radicals generated during the autoxidation of ID-H can directly be trapped on DMPO; (ii) the effect of ID-H on the OH* free radicals is abolished if SOD is added to the system; (iii) the O2(-*) radicals generated by ID-H autoxidation reduce directly the OH-spin adducts on various kinds of nitroxide type spin traps (e.g. TEMPO, TMPN). Hydroxylamine 345-354 isocitrate dehydrogenase (NADP(+)) 1 Homo sapiens 158-162 2172779-3 1990 We have previously shown that cytochrome P-450 in the liver metabolizes sulfamethoxazole to its hydroxylamine metabolite. Hydroxylamine 96-109 cytochrome P450 family 4 subfamily F member 3 Homo sapiens 30-46 2120212-8 1990 The specificity of the mono-ADP-ribosyltransferase zymographic assay was characterized by time course incubations, hydroxylamine sensitivity, 3-aminobenzamide inhibition, and histone dependence. Hydroxylamine 115-128 ecto-ADP-ribosyltransferase 3 Oryctolagus cuniculus 23-50 2398057-8 1990 Treatment of purified delipidated cytochrome b561 with hydroxylamine resulted in the release of a fatty acid hydroxamate. Hydroxylamine 55-68 cytochrome b561 Homo sapiens 34-49 1864444-6 1991 Reversion of the diethylpyrocarbonate reaction with hydroxylamine as well as protection of the enzymes with uroporphyrinogen III indicated that histidine is involved at least in the first decarboxylation active site of the porphyrinogen carboxylyase, and perhaps in one or more sites where the removal of the other carboxyl groups take place. Hydroxylamine 52-65 uroporphyrinogen decarboxylase Rattus norvegicus 223-249 2176823-1 1990 The nitroxide spin label (iodoacetamido)proxyl (IPSL) was specifically and rigidly attached to sulfhydryl 1 (SH1) on myosin subfragment 1 (S1). Hydroxylamine 4-13 myosin heavy chain 14 Homo sapiens 117-123 2143135-11 1990 Rh6 and Iso6 are nearly as stable as rhodopsin towards hydroxylamine and solubilization in detergent solution and could be easily purified and reconstituted into proteoliposomes by established procedures. Hydroxylamine 55-68 rhodopsin Bos taurus 37-46 2171695-14 1990 The sensitivity of hydroxylamine assay is close to the sensitivity of chemiluminescent method, but specificity is higher, as SOD inhibits chemiluminescence only by 50%. Hydroxylamine 19-32 superoxide dismutase 1 Homo sapiens 125-128 2166571-1 1990 A spin-label (P-OPC) composed of the nitroxide-containing ring proxyl linked at the C1 position of the intercalating fluorescent chromophore oxazolopyridocarbazole (OPC) has been synthesized. Hydroxylamine 37-46 spindlin 1 Homo sapiens 2-6 2194573-9 1990 Both fatty acids were also incorporated into CD9 in hydroxylamine-sensitive bonds in the presence of cycloheximide, reaching 30-40% of the levels in untreated controls. Hydroxylamine 52-65 CD9 molecule Homo sapiens 45-48 2364085-5 1990 The rates of reduction were fastest for a six-membered positively charged nitroxide ("CAT-1") and slowest for an anionic five-membered ring nitroxide ("PCA"). Hydroxylamine 74-83 GIT ArfGAP 1 Rattus norvegicus 86-92 1692019-8 1990 The mobility of chymotrypsin in the PZP.chymotrypsin complex was examined by covalently attaching a nitroxide spin label at the serine residue in the active site of the enzyme and examining the appearance of the ESR spectrum. Hydroxylamine 100-109 PZP alpha-2-macroglobulin like Homo sapiens 36-39 2323850-4 1990 During incubation, GAH is metabolized to hydroxylamine. Hydroxylamine 41-54 transglutaminase 2, C polypeptide Mus musculus 19-22 2323850-5 1990 Hydroxylamine acts as the active form of GAH, since the concentration-dependent inhibition of cell growth caused by hydroxylamine is the same as that observed with GAH. Hydroxylamine 0-13 transglutaminase 2, C polypeptide Mus musculus 41-44 2323850-5 1990 Hydroxylamine acts as the active form of GAH, since the concentration-dependent inhibition of cell growth caused by hydroxylamine is the same as that observed with GAH. Hydroxylamine 0-13 transglutaminase 2, C polypeptide Mus musculus 164-167 2323850-5 1990 Hydroxylamine acts as the active form of GAH, since the concentration-dependent inhibition of cell growth caused by hydroxylamine is the same as that observed with GAH. Hydroxylamine 116-129 transglutaminase 2, C polypeptide Mus musculus 41-44 2323850-10 1990 Under these conditions hydroxylamine is highly toxic, suggesting that in vivo GAH might act as an hydroxylamine releaser in the tumor cells and is not significantly metabolized in the body. Hydroxylamine 23-36 transglutaminase 2, C polypeptide Mus musculus 78-81 2323850-10 1990 Under these conditions hydroxylamine is highly toxic, suggesting that in vivo GAH might act as an hydroxylamine releaser in the tumor cells and is not significantly metabolized in the body. Hydroxylamine 98-111 transglutaminase 2, C polypeptide Mus musculus 78-81 2157806-2 1990 The p90-nucleotide bond is most likely to be a phosphodiester linkage, as it resisted treatment with HCl and NH2OH but was sensitive to NaOH. Hydroxylamine 109-114 cellular inhibitor of PP2A Homo sapiens 4-7 2157417-0 1990 Subcellular distribution of a nitroxide spin-labeled netropsin-acridine hybrid in living KB cells: Electron Spin Resonance Study. Hydroxylamine 30-39 spindlin 1 Homo sapiens 40-44 2157417-0 1990 Subcellular distribution of a nitroxide spin-labeled netropsin-acridine hybrid in living KB cells: Electron Spin Resonance Study. Hydroxylamine 30-39 spindlin 1 Homo sapiens 108-112 1688588-6 1990 Nondenaturing polyacrylamide gel electrophoresis of 14C-radiomethylated E protein revealed that pretreatment with hydroxylamine, a reagent which removes ester-linked lipids, rendered it PI-PLC susceptible. Hydroxylamine 114-127 phospholipase C beta 1 Homo sapiens 186-192 2140051-4 1990 In addition, all the iodopsin isomers and analogues were more susceptible to hydroxylamine than were the rhodopsin ones. Hydroxylamine 77-90 opsin 1 (cone pigments), long-wave-sensitive (color blindness, protan) Gallus gallus 21-29 1973271-1 1990 The method for the assay of glutamine synthetase (GlnS) relies on the gamma-glutamyl transferase reaction, i.e. the formation of glutamyl-gamma-hydroxamate from glutamine and hydroxylamine, and the chromatographic separation of the reaction product from the reactants. Hydroxylamine 175-188 glutamate-ammonia ligase Rattus norvegicus 28-48 1973271-1 1990 The method for the assay of glutamine synthetase (GlnS) relies on the gamma-glutamyl transferase reaction, i.e. the formation of glutamyl-gamma-hydroxamate from glutamine and hydroxylamine, and the chromatographic separation of the reaction product from the reactants. Hydroxylamine 175-188 glutamate-ammonia ligase Rattus norvegicus 50-54 1973271-1 1990 The method for the assay of glutamine synthetase (GlnS) relies on the gamma-glutamyl transferase reaction, i.e. the formation of glutamyl-gamma-hydroxamate from glutamine and hydroxylamine, and the chromatographic separation of the reaction product from the reactants. Hydroxylamine 175-188 gamma-glutamyltransferase 1 Rattus norvegicus 70-96 2154454-5 1990 This nitroxide, which we have assigned as PBN/.OCH3, appears to be an oxygen-centered radical derived from the spin trapping of the reaction product of O2 and methyl radical. Hydroxylamine 5-14 spindlin 1 Homo sapiens 111-115 2125422-3 1990 Activity variations with respect to pH, temperature, stability behaviour and the inhibition effect of hydroxylamine were investigated for the immobilized catalase preparations. Hydroxylamine 102-115 catalase Homo sapiens 154-162 2157810-5 1990 Additions of Ca(II) and Cd(II) to spin-labeled apo-calmodulin gave rise to very similar changes in the EPR spectrum of the bound label, consistent with a dramatic decrease in the mobility of the nitroxide spin-label covalently attached to tyrosine-99. Hydroxylamine 195-204 carbonic anhydrase 2 Bos taurus 13-19 2157810-5 1990 Additions of Ca(II) and Cd(II) to spin-labeled apo-calmodulin gave rise to very similar changes in the EPR spectrum of the bound label, consistent with a dramatic decrease in the mobility of the nitroxide spin-label covalently attached to tyrosine-99. Hydroxylamine 195-204 calmodulin Bos taurus 51-61 1965624-3 1990 An average of six nitroxides were bound covalently to each molecule of human serum albumin. Hydroxylamine 18-28 albumin Homo sapiens 77-90 2323183-2 1990 For both types of erythrocytes, activated C3b bound in a diffuse pattern via hydroxylamine-sensitive ester bonds. Hydroxylamine 77-90 endogenous retrovirus group K member 3 Homo sapiens 42-45 2167277-0 1990 Principles of the metabolism of nitroxides and their implications for spin trapping. Hydroxylamine 32-42 spindlin 1 Homo sapiens 70-74 2167277-1 1990 A review of the principal interactions of nitroxides with cells suggests that if these same phenomena occur with spin adducts the result could be considerable experimental confusion and error. Hydroxylamine 42-52 spindlin 1 Homo sapiens 113-117 2167277-3 1990 In addition, shuttling of electrons between nitroxides and hydroxylamines also very significantly could alter the amounts and types of spin adducts that are observed. Hydroxylamine 44-54 spindlin 1 Homo sapiens 135-139 2286824-3 1990 Prostacyclin (PG12), a powerful vasodilator, and the most potent known inhibitor of platelet aggregation, derived from arachidonic acid metabolism: 2: Endothelium-derived relaxing factor (EDRF) a powerful vasodilator thought to be a short-acting nitroxide; 3: Endothelin, a 21 amino-acid peptide with potent vasoconstricting properties. Hydroxylamine 246-255 alpha hemoglobin stabilizing protein Homo sapiens 151-186 1966818-9 1990 The inhibition of PAF synthesis by hydroxylamine is reversed by adding acetyl-CoA. Hydroxylamine 35-48 PCNA clamp associated factor Homo sapiens 18-21 2286824-3 1990 Prostacyclin (PG12), a powerful vasodilator, and the most potent known inhibitor of platelet aggregation, derived from arachidonic acid metabolism: 2: Endothelium-derived relaxing factor (EDRF) a powerful vasodilator thought to be a short-acting nitroxide; 3: Endothelin, a 21 amino-acid peptide with potent vasoconstricting properties. Hydroxylamine 246-255 alpha hemoglobin stabilizing protein Homo sapiens 188-192 3275469-4 1988 The radiolabelled fatty acid in CD9 appears to be ester bonded, as it is removed by treatment with hydroxylamine. Hydroxylamine 99-112 CD9 molecule Homo sapiens 32-35 10945437-0 2000 Atomic structures of two nitroxide spin labels complexed with human thrombin: comparison with solution studies. Hydroxylamine 25-34 coagulation factor II, thrombin Homo sapiens 68-76