PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 22567568-1 2012 Alphalactalbumin (alpha-La) and betalactoglobulin (beta-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114. Nonidet P-40 188-200 lactalbumin alpha Bos taurus 0-16 22567568-1 2012 Alphalactalbumin (alpha-La) and betalactoglobulin (beta-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114. Nonidet P-40 188-200 beta-lactoglobulin Bos taurus 32-49 22567568-3 2012 The optimized conditions for cloud point extraction of alphalactalbumin (alpha-La) and betalactoglobulin (beta-Lg) can be concluded that the best surfactant is 1% (w/v) Triton X-114, 200 muL of sample volume, 150 mmol/L NaCl, and 6% (w/v) sucrose. Nonidet P-40 169-181 lactalbumin alpha Bos taurus 55-71 23119070-6 2012 Proteolysis analyses, Triton X-114 partitioning and agglutination assays showed that the N-terminal part of ScARP3d, consisting of repeated sequences, was exposed to the spiroplasma surface whereas the C-terminal part was anchored into the membrane. Nonidet P-40 22-34 adhesion related protein, transmembrane Spiroplasma citri 108-115 21479916-3 2011 Fractionation of membranes from human cell lines, of neural and non-neural origin, by temperature-induced phase separation in Triton X-114 resulted in the detection of DDC molecules in all separation phases. Nonidet P-40 126-138 dopa decarboxylase Homo sapiens 168-171 22216323-6 2011 Sub-cellular fractionation and Triton X-114 membrane phase separation analysis indicated that ZFYVE27 is a peripheral membrane protein. Nonidet P-40 31-43 zinc finger FYVE-type containing 27 Homo sapiens 94-101 21337516-10 2011 These studies reveal that the application of CCS to first isolate Ad and nAd PM fractions, followed by their detergent-phase TX-114 partitioning, to be a powerful method to isolate low-abundance PM proteins, and a useful adjunct for in-depth cell surface proteome analyses. Nonidet P-40 125-131 copper chaperone for superoxide dismutase Canis lupus familiaris 45-48 21740893-5 2011 In both cell types, Hyal2 was strongly associated with cell membrane fractions from which it could be extracted using a Triton X-114 treatment (hydrophobic phase) but not an osmotic shock or an alkaline carbonate solution. Nonidet P-40 120-132 hyaluronidase 2 Homo sapiens 20-25 21476293-6 2011 Addition of 0.1% of NP-40 to the radioimmunoassay buffer markedly reduced non-specific binding of both labeled and unlabeled salusin-beta to the assay tubes without interfering the binding of salusin-beta to its antibody. Nonidet P-40 20-25 torsin family 2 member A Homo sapiens 125-137 20926308-6 2011 Upon Triton X-114 partitioning, most of the IL-32 partitioned to the detergent phase, suggesting hydrophobic characteristics. Nonidet P-40 5-17 interleukin 32 Homo sapiens 44-49 20053954-6 2010 The MAM, which is highly capable of accumulating ceramides, is enriched with both cholesterol and simple sphingolipids, thus forming Triton X-114-resistant DRMs. Nonidet P-40 133-145 sarcoglycan gamma Homo sapiens 4-7 19297523-3 2009 We have developed two novel assays to assess Cx43 folding and assembly: acquisition of resistance of disulfide bonds to reduction by extracellularly added DTT and Triton X-114 detergent phase partitioning. Nonidet P-40 163-175 gap junction protein alpha 1 Homo sapiens 45-49 20137110-1 2010 This study was purposed to investigate the expression and significance of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) in bone marrow mononuclear cells (BMMNC) isolated from patients with acute myeloid leukemia (AML), GPI-PLD activity in BMMNC isolated from 78 patients with AML and 15 normal persons was measured by using GPI-anchored placental alkaline phosphatase (PLAP) as a substrate and Triton X-114 phase partitioning. Nonidet P-40 412-424 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 74-127 20137110-1 2010 This study was purposed to investigate the expression and significance of glycosylphosphatidylinositol-specific phospholipase D (GPI-PLD) in bone marrow mononuclear cells (BMMNC) isolated from patients with acute myeloid leukemia (AML), GPI-PLD activity in BMMNC isolated from 78 patients with AML and 15 normal persons was measured by using GPI-anchored placental alkaline phosphatase (PLAP) as a substrate and Triton X-114 phase partitioning. Nonidet P-40 412-424 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 129-136 19797320-6 2010 Protein extraction with Triton X-114 and sodium carbonate and cross-linking experiments demonstrate that both forms of NEU4 are extrinsic membrane proteins, anchored via protein-protein interactions. Nonidet P-40 24-36 neuraminidase 4 Homo sapiens 119-123 18390998-5 2008 BBA65, BBA66, BBA71, and BBA73 proteins were also found to partition into the Triton X-114 detergent phase and were sensitive to protease treatment of intact cells, indicating that they are membrane associated and surface localized. Nonidet P-40 78-90 complement regulator-acquiring protein Borreliella burgdorferi B31 0-5 19467631-2 2009 S2 cells stably transfected with the Drosophila NTPDase6 cDNA displayed strong UDPase activity only after addition of NP-40, indicating the intracellular location of the enzyme. Nonidet P-40 118-123 NTPase Drosophila melanogaster 48-56 20032577-3 2009 METHODS: Unprocessed and geranylgeranylated forms of RhoA and Rac1 in aortic atherosclerotic lesions were separated by the Triton X-114 partition method using Watanabe heritable hyperlipidemic (WHHLMI) rabbits prone to myocardial infarction. Nonidet P-40 123-135 transforming protein RhoA Oryctolagus cuniculus 53-57 20032577-3 2009 METHODS: Unprocessed and geranylgeranylated forms of RhoA and Rac1 in aortic atherosclerotic lesions were separated by the Triton X-114 partition method using Watanabe heritable hyperlipidemic (WHHLMI) rabbits prone to myocardial infarction. Nonidet P-40 123-135 ras-related C3 botulinum toxin substrate 1 Oryctolagus cuniculus 62-66 19218237-2 2009 We recently demonstrated that a lipoprotein fraction obtained from S. aureus by Triton X-114 phase partitioning is a potent activator of TLR2. Nonidet P-40 80-92 toll like receptor 2 Homo sapiens 137-141 19041269-5 2009 Treatment of white blood cells with Triton X-114 resulted in the recovery of DDC in the detergent enriched and highly hydrophobic phases, suggesting association of DDC molecules with membranes in the studied cells. Nonidet P-40 36-48 dopa decarboxylase Homo sapiens 77-80 19041269-5 2009 Treatment of white blood cells with Triton X-114 resulted in the recovery of DDC in the detergent enriched and highly hydrophobic phases, suggesting association of DDC molecules with membranes in the studied cells. Nonidet P-40 36-48 dopa decarboxylase Homo sapiens 164-167 18390998-5 2008 BBA65, BBA66, BBA71, and BBA73 proteins were also found to partition into the Triton X-114 detergent phase and were sensitive to protease treatment of intact cells, indicating that they are membrane associated and surface localized. Nonidet P-40 78-90 complement regulator-acquiring protein Borreliella burgdorferi B31 7-12 18390998-5 2008 BBA65, BBA66, BBA71, and BBA73 proteins were also found to partition into the Triton X-114 detergent phase and were sensitive to protease treatment of intact cells, indicating that they are membrane associated and surface localized. Nonidet P-40 78-90 complement regulator-acquiring protein Borreliella burgdorferi B31 14-19 18390998-5 2008 BBA65, BBA66, BBA71, and BBA73 proteins were also found to partition into the Triton X-114 detergent phase and were sensitive to protease treatment of intact cells, indicating that they are membrane associated and surface localized. Nonidet P-40 78-90 complement regulator-acquiring protein Borreliella burgdorferi B31 25-30 17252234-2 2007 Globomycin treatment, Triton X-114 separation and mass spectrometry analyses clarified a property of the Rv0679c protein as a lipoprotein. Nonidet P-40 22-34 hypothetical protein Mycobacterium tuberculosis H37Rv 105-112 18191935-3 2008 S. aureus LP (saLP) prepared by Triton X-114 extraction stimulated the activation of NF-kappaB, JNK, and P38 signaling pathways in HUCL cells. Nonidet P-40 32-44 ELAV like RNA binding protein 3 Homo sapiens 131-135 17989073-9 2008 Recombinant human NPC1 also bound [(3)H]cholesterol in a reaction inhibited by Nonidet P-40 above its critical micellar concentration. Nonidet P-40 79-91 NPC intracellular cholesterol transporter 1 Homo sapiens 18-22 17615295-5 2007 Triton X-114 phase separation and [(3)H]inositol labeling indicated that the glycosylphosphatidylinositol (GPI)-anchoring was defective in the pga1(ts) mutants, suggesting that Pga1 is involved in GPI synthesis or its transfer to target proteins. Nonidet P-40 0-12 Pga1p Saccharomyces cerevisiae S288C 143-147 18044716-10 2008 Upon lowering pH in the presence of EGTA recombinant AnxA6-2 became less hydrophobic than AnxA6-1 as revealed by the Triton X-114 partition. Nonidet P-40 117-129 annexin A6 Mus musculus 53-58 18326903-6 2008 GPI-PLD activities were analyzed quantitatively by triton-X-114 partition with GPI anchored placental alkaline phosphatase (PLAP) as a substrate. Nonidet P-40 51-63 glucose-6-phosphate isomerase Homo sapiens 0-3 17881279-3 2008 The Abl-RACK1 of transformed cells as resolvable with Triton X-114 was found with little affinity for FAK, PY(397)-FAK and integrin. Nonidet P-40 54-66 c-abl oncogene 1, non-receptor tyrosine kinase Mus musculus 4-7 17881279-3 2008 The Abl-RACK1 of transformed cells as resolvable with Triton X-114 was found with little affinity for FAK, PY(397)-FAK and integrin. Nonidet P-40 54-66 receptor for activated C kinase 1 Mus musculus 8-13 17535983-9 2007 We also found an increase in the amount of p85 associated with the Nonidet P-40-insoluble fraction derived from MSU crystal-stimulated human neutrophils. Nonidet P-40 67-79 phosphoinositide-3-kinase regulatory subunit 2 Homo sapiens 43-46 17400609-9 2007 hCAP-18 surface association was labile to 10 mM NaOH treatment but resistant to 1 M NaCl and also partitioned into the detergent phase following Triton X-114 solubilization, possibly suggesting a stable association with one or more integral membrane proteins. Nonidet P-40 145-157 cathelicidin antimicrobial peptide Homo sapiens 0-7 16562670-4 2006 and GPI-PLD activities were determined by GPI-anchored placental alkaline phosphatase (PLAP) as substrate and triton-X114 partioning. Nonidet P-40 110-121 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 4-11 16464866-4 2006 PSDP phosphatase activity was identified in activated human neutrophil (PMN) extracts and partially purified in the presence of Nonidet P-40 with gel filtration and anion exchange chromatography. Nonidet P-40 128-140 phospholipid phosphatase 6 Homo sapiens 0-4 16870943-6 2006 In the Ace-/- mice, while ADAM2 was found normally in the sperm, ADAM3 disappeared from the Triton X-114 detergent-enriched phase after phase separation, which suggests that ACE is involved in distributing ADAM3 to a location where it can participate in sperm-zona pellucida binding. Nonidet P-40 92-104 a disintegrin and metallopeptidase domain 3 (cyritestin) Mus musculus 65-70 16870943-6 2006 In the Ace-/- mice, while ADAM2 was found normally in the sperm, ADAM3 disappeared from the Triton X-114 detergent-enriched phase after phase separation, which suggests that ACE is involved in distributing ADAM3 to a location where it can participate in sperm-zona pellucida binding. Nonidet P-40 92-104 angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 Mus musculus 174-177 16870943-7 2006 This diminished amount of ADAM3 in the Triton X-114 detergent-enriched phase may explain the inability of Clgn-/- and Ace-/- sperm to bind to the zona pellucida. Nonidet P-40 39-51 a disintegrin and metallopeptidase domain 3 (cyritestin) Mus musculus 26-31 16029000-1 2005 Dominga grape polyphenol oxidase (PPO) was extracted using phase partitioning with Triton X-114. Nonidet P-40 83-95 protoporphyrinogen oxidase Homo sapiens 14-32 16303976-7 2005 In addition, TULP1 partitions to the aqueous phase during Triton X-114 extraction. Nonidet P-40 58-70 TUB like protein 1 Homo sapiens 13-18 15993753-5 2005 Here, we report the identification of an integral plasma membrane pool for all the 10 PKC isozymes in vivo by their ability to partition into the detergent-rich phase in Triton X-114 phase partitioning, and by their resistance to extractions with 0.2 M sodium carbonate (pH 11.5), 2 M urea and 2 M sodium chloride. Nonidet P-40 170-182 protein kinase C alpha Homo sapiens 86-89 16029000-1 2005 Dominga grape polyphenol oxidase (PPO) was extracted using phase partitioning with Triton X-114. Nonidet P-40 83-95 protoporphyrinogen oxidase Homo sapiens 34-37 15640525-3 2005 The amounts of the unprocessed and geranylgeranylated forms of RhoA in non-stimulated cultured human aortic ECs were 31 +/- 8 and 69 +/- 8% total cellular RhoA, respectively (n = 6, p < 0.0001), as determined by the Triton X-114 partition method. Nonidet P-40 219-231 ras homolog family member A Homo sapiens 63-67 15234983-2 2004 We identified more than 250 proteins associated with Nonidet P-40 soluble AS, and demonstrated that at least 51 of these proteins displayed significant differences in their relative abundance in AS complexes under conditions where rotenone was cytotoxic and induced formation of cytoplasmic inclusions immunoreactive to anti-AS. Nonidet P-40 53-65 synuclein alpha Homo sapiens 195-197 15342784-5 2004 SMP-1 is associated with detergent-resistant membranes based on its recovery in the buoyant fraction after Triton X-100 extraction and sucrose density centrifugation and coextraction with the major surface glycolipids in Triton X-114. Nonidet P-40 221-233 transmembrane protein 50A Homo sapiens 0-5 15843158-5 2005 A combination of Triton X-114 phase separation and nickel-affinity chromatography yielded exclusively prenylated Rab38 that bound [alpha-32P]-GTP. Nonidet P-40 17-29 RAB38, member RAS oncogene family Rattus norvegicus 113-118 15060073-6 2004 Interestingly solubilizing membrane expressing CFTR in detergents such as Triton X-100, Nonidet P-40, deoxycholate, and SDS tended to destabilize the CFTR dimers and dissociate them into monomeric form. Nonidet P-40 88-100 CF transmembrane conductance regulator Homo sapiens 47-51 15205586-6 2004 Using a detergent-phase separation method with a Triton X-114-containing buffer, we could demonstrate that the levels of membrane (glycosylphosphatidylinositol)-anchored UPAR were significantly higher in the intima of early atherosclerotic lesions as well as in the cap areas of fibrous plaques compared with macroscopically normal areas. Nonidet P-40 49-61 plasminogen activator, urokinase receptor Homo sapiens 170-174 15336428-3 2004 The uptake and esterification of external [4-14C]-cholesterol is strongly reduced in hem1 mutants treated with low concentrations of Nonidet P-40. Nonidet P-40 133-145 5-aminolevulinate synthase Saccharomyces cerevisiae S288C 85-89 15060073-6 2004 Interestingly solubilizing membrane expressing CFTR in detergents such as Triton X-100, Nonidet P-40, deoxycholate, and SDS tended to destabilize the CFTR dimers and dissociate them into monomeric form. Nonidet P-40 88-100 CF transmembrane conductance regulator Homo sapiens 150-154 15039353-5 2004 It detected BmpA in the Triton X-114-soluble and -insoluble fractions of B. burgdorferi, suggesting association with both inner and outer bacterial cell membranes. Nonidet P-40 24-36 BMP family protein Borreliella burgdorferi B31 12-16 15075238-6 2004 The upstream activator MEK5 is also localized in the nucleus both before and after EGF stimulation and is resistant to NP-40 extraction in resting cells. Nonidet P-40 119-124 mitogen-activated protein kinase kinase 5 Homo sapiens 23-27 14685283-2 2004 We show here that all Env subunits of the virus form disulphide-linked SU-TM complexes that can be disrupted by treatment with NP-40, heat or urea, or by Ca(2+) depletion. Nonidet P-40 127-132 melanoma antigen Mus musculus 22-25 12946943-4 2004 Nonidet P-40-insoluble (i.e., TJ-associated) occludin and ZO-1 were virtually undetectable 12 and 18 h after injecting LPS. Nonidet P-40 0-12 occludin Mus musculus 45-53 14972104-10 2004 The membrane association of re-mZP3 was confirmed by the finding that biotinylated re-mZP3 (approximately 75 kDa) is immunoprecipitated from the hydrophobic phase of Triton X-114 extracts of transfected cells following phase partitioning. Nonidet P-40 166-178 zona pellucida glycoprotein 3 Mus musculus 31-35 14972104-10 2004 The membrane association of re-mZP3 was confirmed by the finding that biotinylated re-mZP3 (approximately 75 kDa) is immunoprecipitated from the hydrophobic phase of Triton X-114 extracts of transfected cells following phase partitioning. Nonidet P-40 166-178 zona pellucida glycoprotein 3 Mus musculus 86-90 12881314-6 2003 Moreover, we found that exosome-associated Lyn (1) had a lower molecular weight compared with Lyn detected in cell-isolated detergent-resistant domains, (2) was absent from the Triton X-100-insoluble fraction isolated from exosomes, and (3) had lost its partitioning capacity in Triton X-114. Nonidet P-40 279-291 LYN proto-oncogene, Src family tyrosine kinase Homo sapiens 43-46 12663329-8 2003 Phase separation using Triton X-114 showed that alpha-SNAP partitioned into both aqueous and detergent phases. Nonidet P-40 23-35 NSF attachment protein alpha Homo sapiens 48-58 14647034-8 2003 On phase separation with Triton X-114, the soluble stool DAF was partitioned mainly into the aqueous phase, indicating its hydrophilic nature and lack of the fatty-acid glycosylphosphatidylinositol anchor component. Nonidet P-40 25-37 CD55 molecule (Cromer blood group) Homo sapiens 57-60 14602200-3 2003 Following extraction with 1% Triton X-114, NS3 was predominantly present in the aqueous phase, but NS2-3 was only recovered in the detergent phase. Nonidet P-40 29-41 KRAS proto-oncogene, GTPase Homo sapiens 43-46 12110545-6 2002 Third, by using phase transition of Triton X-114, the purified GLUT4 was found to be partly detergent resistant, which is a known characteristic of GPI-linked proteins. Nonidet P-40 36-48 solute carrier family 2 member 4 Rattus norvegicus 63-68 12780349-6 2003 Consistently, sodium carbonate extraction and Triton X-114 treatment showed that torsinA is associated with the ER membrane in CHO (Chinese-hamster ovary) cells. Nonidet P-40 46-58 torsin family 1 member A Homo sapiens 81-88 12837618-5 2003 Metabolic labeling with [(3)H]palmitate and Triton-X-114 phase distribution proved that SNAP-25 is palmitoylated in significant amounts. Nonidet P-40 44-56 synaptosome associated protein 25 Homo sapiens 88-95 11939716-6 2002 When the experiments were conducted in the presence of 5mM EDTA, the GPI-PLD inhibitor, the majority of radiolabeled MBP remained in the membrane-bound form and was extractable with Triton X- 114. Nonidet P-40 182-195 glycosylphosphatidylinositol specific phospholipase D1 Rattus norvegicus 69-76 12058062-7 2002 Triton X-114 phase separation revealed that an increased amount of tyrosinase was proteolyzed in melan-p1 cells compared with wild-type melanocytes. Nonidet P-40 0-12 tyrosinase Homo sapiens 67-77 11939716-6 2002 When the experiments were conducted in the presence of 5mM EDTA, the GPI-PLD inhibitor, the majority of radiolabeled MBP remained in the membrane-bound form and was extractable with Triton X- 114. Nonidet P-40 182-195 myelin basic protein Rattus norvegicus 117-120 11851431-3 2002 In partitioning studies in Triton X-114 solution, RTA is predominantly found in the detergent phase, whereas ricin holotoxin, native RTB, and several single-chain ribosome-inactivating proteins (RIPs) are in the aqueous phase. Nonidet P-40 27-39 MAS related GPR family member F Homo sapiens 50-53 11893086-2 2002 Based on Triton X-114 partition, carbonate extraction and trypsin protection assays, p54 behaved as an extrinsic membrane protein, facing the luminal compartment. Nonidet P-40 9-21 interferon induced protein with tetratricopeptide repeats 2 Homo sapiens 85-88 11869093-3 2002 The released form of CAP was hydrophilic as assessed by phase separation in Triton X-114 and high-speed centrifugation. Nonidet P-40 76-88 leucyl and cystinyl aminopeptidase Homo sapiens 21-24 10950880-7 2000 Western blotting analysis of both NP-40-soluble and detergent/high-salt insoluble fractions of isolated islets of Langerhans allowed detection of GFAP in both cytosolic and cytoskeletal compartments. Nonidet P-40 34-39 glial fibrillary acidic protein Mus musculus 146-150 11513473-5 2001 Fractionation of membranes by temperature-induced phase separation in Triton X-114, resulted in the recovery of membrane-associated DDC in separation phases where integral and hydrophobic membrane proteins separate. Nonidet P-40 70-82 dopa decarboxylase Homo sapiens 132-135 11163598-2 2001 VEGF was extracted efficiently and reproducibly from muscle homogenate with low concentrations of non-ionic detergents, such as Triton X-100, Nonidet P-40, and Tween 20. Nonidet P-40 142-154 vascular endothelial growth factor A Rattus norvegicus 0-4 11071917-6 2000 Using subcellular fractionation, Triton X-114 phase separation, protease protection assays, and immunofluorescence microscopy (IF), we have identified that FKBP65 is contained within the lumen of the endoplasmic reticulum (ER). Nonidet P-40 33-45 FK506 binding protein 10 Mus musculus 156-162 10842180-6 2000 Using Triton X-114 phase partitioning to enrich for glycosylphosphatidylinositol-anchored proteins, Dpl was detected in brain samples from Rcm0 Prnp(0/0) mice but was absent in equivalent samples from wt mice and ZrchI Prnp(0/0) mice, indicating that ectopic expression of this protein may cause cerebellar pathology in Rcm0 mice. Nonidet P-40 6-18 prion like protein doppel Mus musculus 100-103 10792497-5 2000 The mAb precipitated a protein of 86 000 MW under non-reducing conditions and a doublet of 43 000 MW under reducing conditions from peripheral blood T lymphocytes lysed in nonidet P-40 buffer, whilst it precipitated the CD3-TCR complex from the cells lysed in digitonin. Nonidet P-40 172-184 CD3 epsilon subunit of T-cell receptor complex Sus scrofa 220-223 10906204-3 2000 Localization of the H3L protein on the surfaces of viral particles and anchorage via the hydrophobic tail were consistent with its extraction by NP-40 in the absence of reducing agents, its trypsin sensitivity, its reactivity with a membrane-impermeable biotinylation reagent, and its immunogold labeling with an antibody to a peptide comprising amino acids 247 to 259. Nonidet P-40 145-150 H3 clustered histone 2 Homo sapiens 20-23 10753942-3 2000 When membranes were subjected to Triton X-114 partitioning, syncollin was found predominantly in the aqueous phase, indicating that it is not sufficiently hydrophobic to be embedded in the membrane. Nonidet P-40 33-45 syncollin Homo sapiens 60-69 9886898-7 1998 The mesangial cells were collected and ACE enzyme was extracted using Triton X-114, followed by centrifugation and concentration. Nonidet P-40 70-82 angiotensin I converting enzyme Rattus norvegicus 39-42 10491642-6 1999 The presence of the GPI anchor on ceruloplasmin was confirmed by Triton X-114 phase partitioning experiments and by recognition with an antibody to the GPI anchor. Nonidet P-40 65-77 ceruloplasmin Rattus norvegicus 34-47 10490457-7 1999 Consistent with localization studies, biochemical analyses revealed that DLK/MUK/ZPK was predominantly associated with Golgi membranes on fractionation of cellular extracts and was entirely partitioned into the aqueous phase when membranes were subjected to Triton X-114 extraction. Nonidet P-40 258-270 mitogen-activated protein kinase kinase kinase 12 Mus musculus 73-76 10490457-7 1999 Consistent with localization studies, biochemical analyses revealed that DLK/MUK/ZPK was predominantly associated with Golgi membranes on fractionation of cellular extracts and was entirely partitioned into the aqueous phase when membranes were subjected to Triton X-114 extraction. Nonidet P-40 258-270 mitogen-activated protein kinase kinase kinase 12 Mus musculus 77-80 10490457-7 1999 Consistent with localization studies, biochemical analyses revealed that DLK/MUK/ZPK was predominantly associated with Golgi membranes on fractionation of cellular extracts and was entirely partitioned into the aqueous phase when membranes were subjected to Triton X-114 extraction. Nonidet P-40 258-270 mitogen-activated protein kinase kinase kinase 12 Mus musculus 81-84 10488083-9 1999 Using temperature-induced phase partitioning with Triton X-114, NRP was associated with both the insoluble membrane skeleton pellet and the soluble aqueous phase but not the soluble detergent phase. Nonidet P-40 50-62 Nrp Rattus norvegicus 64-67 10446390-4 1999 GAP-43 partitioned into Triton X-114 in the presence of plasma membrane, Golgi, and ERGIC membranes, but not nuclei or mitochondria. Nonidet P-40 24-36 growth associated protein 43 Homo sapiens 0-6 9882312-7 1999 Palmitylation, oleation, and partitioning of the F13L protein in Triton X-114 detergent were unaffected by the K314R substitution. Nonidet P-40 65-77 palmytilated EEV membrane glycoprotein Vaccinia virus 49-53 10777113-8 2000 Assays involving proteinase K digestion, sodium carbonate extraction and Triton X-114 extraction revealed that the insect CALNUC-like protein was a soluble protein tightly associated with the luminal surface of Golgi membranes as reported for mammalian CALNUC. Nonidet P-40 73-85 nucleobindin 1 Homo sapiens 122-128 10620504-8 2000 Triton X-100, Nonidet P-40 and n-dedecyl maltoside also activated monomeric Bax, whereas CHAPS had no activating effect. Nonidet P-40 14-26 BCL2 associated X, apoptosis regulator Homo sapiens 76-79 10601283-4 1999 Furthermore, in GAD65-/- mouse brain, GAD67, which no longer partitions into the Triton X-114 detergent phase, still anchors to membranes at similar levels as in wild-type mice. Nonidet P-40 81-93 glutamic acid decarboxylase 2 Mus musculus 16-21 10601283-4 1999 Furthermore, in GAD65-/- mouse brain, GAD67, which no longer partitions into the Triton X-114 detergent phase, still anchors to membranes at similar levels as in wild-type mice. Nonidet P-40 81-93 glutamate decarboxylase 1 Rattus norvegicus 38-43 10632578-2 1999 Synaptobrevin-2 was localized in the lamellar bodies, and syntaxin-1 and SNAP-25 were found in 0.4% Nonidet P40-soluble and -insoluble fractions, respectively, of the type II cells. Nonidet P-40 100-111 synaptosome associated protein 25 Rattus norvegicus 73-80 10194365-8 1999 By Triton X-114 partitioning, it was confirmed that calcium increases the hydrophobicity of 5LO. Nonidet P-40 3-15 arachidonate 5-lipoxygenase Homo sapiens 92-95 10191115-3 1999 By using Western blotting, Percoll density gradient fractionation, and Triton X-114 extraction, we demonstrate that the product of the CLN3 gene, which we call battenin, in mammalian expression system studied is a highly glycosylated protein of lysosomal membrane. Nonidet P-40 71-83 CLN3 lysosomal/endosomal transmembrane protein, battenin Homo sapiens 135-139 10191115-3 1999 By using Western blotting, Percoll density gradient fractionation, and Triton X-114 extraction, we demonstrate that the product of the CLN3 gene, which we call battenin, in mammalian expression system studied is a highly glycosylated protein of lysosomal membrane. Nonidet P-40 71-83 CLN3 lysosomal/endosomal transmembrane protein, battenin Homo sapiens 160-168 11938765-2 1999 Serum GPI-PLD activity was determined both qualitatively and quantitatively by PEG/dextran and triton-X-114 partitioning respectively, and some kinetic properties of its enzymatic reaction were also studied. Nonidet P-40 95-107 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 6-13 9644224-4 1998 However, after cell volume expansion by exposure to hypotonic medium, ICln rapidly translocates to the particulate fraction (the Triton X-114-insoluble fraction). Nonidet P-40 129-141 chloride nucleotide-sensitive channel 1A Rattus norvegicus 70-74 9740329-3 1998 The c-Abl mAb predominantly recognized two protein bands of 145 kD and 95 kD in detergent-solubilized (Triton X-100 and NP-40) sperm and testes preparations in the Western blot procedure. Nonidet P-40 120-125 ABL proto-oncogene 1, non-receptor tyrosine kinase Homo sapiens 4-9 9675234-6 1998 The hydrodynamic behaviour, concentration in Triton X-114, binding to phenyl-agarose, and sensitivity to phosphatidylinositol-specific phospholipase C revealed that enzyme forms in F-I or F-II were amphiphilic dimers with linked phosphatidylinositol residues, whilst most of NT forms in UF were hydrophilic dimers. Nonidet P-40 45-57 coagulation factor II Mus musculus 188-192 9653976-0 1998 Separation of microsomal cytochrome b5 via phase separation in a mixed solution of Triton X-114 and charged dextran. Nonidet P-40 83-95 cytochrome b5 type A Homo sapiens 25-38 9553144-6 1998 Detergents such as Triton X-100 and Triton X-114 readily enable Bax hetero- and homodimerization. Nonidet P-40 36-48 BCL2-associated X protein Mus musculus 64-67 9653976-1 1998 The successful introduction of a charged dextran into the Triton X-114 phase separation system for the selective extraction of cytochrome b5 (cyt. Nonidet P-40 58-70 cytochrome b5 type A Homo sapiens 127-140 9516432-16 1998 Under conditions that promote binding of p23 to hsp90 (elevated temperature, ATP, Nonidet P-40, molybdate), a five-membered (p23. Nonidet P-40 82-94 prostaglandin E synthase 3 Homo sapiens 41-44 9504959-5 1998 Temperature-sensitive phase distribution (in Triton X-114) allowed separation of soluble from insoluble ALP activity. Nonidet P-40 45-57 alkaline phosphatase, placental Homo sapiens 104-107 9506996-6 1998 Proteolytic processing of caspases-3 and -7 and the caspase substrates poly(ADP-ribose) polymerase, GDP dissociation inhibitor, and PKCdelta was observed when PBLs were activated in vitro, and lysates were prepared using RIPA buffer which contains 1% Nonidet P-40, 0.5% deoxycholate, and 0.1% SDS. Nonidet P-40 251-263 caspase 3 Homo sapiens 26-43 9506996-6 1998 Proteolytic processing of caspases-3 and -7 and the caspase substrates poly(ADP-ribose) polymerase, GDP dissociation inhibitor, and PKCdelta was observed when PBLs were activated in vitro, and lysates were prepared using RIPA buffer which contains 1% Nonidet P-40, 0.5% deoxycholate, and 0.1% SDS. Nonidet P-40 251-263 protein kinase C delta Homo sapiens 132-140 9516432-16 1998 Under conditions that promote binding of p23 to hsp90 (elevated temperature, ATP, Nonidet P-40, molybdate), a five-membered (p23. Nonidet P-40 82-94 Hsp90 family chaperone HSP82 Saccharomyces cerevisiae S288C 48-53 9516432-16 1998 Under conditions that promote binding of p23 to hsp90 (elevated temperature, ATP, Nonidet P-40, molybdate), a five-membered (p23. Nonidet P-40 82-94 prostaglandin E synthase 3 Homo sapiens 125-128 9367512-0 1997 Purification of Wegener"s granulomatosis autoantigen, proteinase 3, from neutrophils by Triton X-114 extraction of azurophilic granules. Nonidet P-40 88-100 proteinase 3 Homo sapiens 54-66 9182670-6 1997 When cells were grown in low Ca medium, almost all occludin was NP-40 soluble. Nonidet P-40 64-69 occludin Canis lupus familiaris 51-59 9390434-0 1997 The characterization of plasma membrane-bound tubulin of cauliflower using Triton X-114 fractionation. Nonidet P-40 75-87 tubulin beta-4 chain Brassica oleracea 46-53 9390434-4 1997 Approximately one-half of the PM-associated tubulin was solubilized by Triton X-114 and 10 to 15% of both alpha- and beta-tubulin was recovered in the detergent phase (indicative of hydrophobic properties) and 30 to 40% was recovered in the aqueous phase. Nonidet P-40 71-83 tubulin beta-4 chain Brassica oleracea 44-51 9260929-4 1997 We demonstrate that lactadherin purified using Triton X-114 phase partitioning promotes RGD-dependent cell attachment of green monkey kidney cells (MA104), mouse fibroblast cells (3T3-L1), and breast carcinoma cells (ELL-G). Nonidet P-40 47-59 milk fat globule EGF and factor V/VIII domain containing Homo sapiens 20-31 9615614-3 1997 For n-butanol extracts containing oil red, 5"-nucleotidase, or alkaline phosphatase, the hydrophobic molecules and Triton X-114 were retained in the aqueous phase during incubations at 30 degrees C. The n-butanol interference was concentration-dependent and was reduced by lowering the final n-butanol concentration of the sample to 1.5% (v/v) or less. Nonidet P-40 115-127 5'-nucleotidase ecto Homo sapiens 43-58 9013878-4 1997 Moreover, Triton X-114 phase partitioning performed on inner membranes showed that PDPc behaved both as a hydrophilic and as a hydrophobic protein, thus suggesting two different forms of this enzyme. Nonidet P-40 10-22 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 83-87 9218769-8 1997 BlyA was highly purified from E. coli in a single step utilizing Triton X-114 phase partitioning. Nonidet P-40 65-77 holin BlyA Borreliella burgdorferi B31 0-4 9218131-6 1997 This fraction of annexin II was also extracted by 0.1 M Na2CO3, pH 11 and partitioned into the aqueous phase after being treated with Triton X-114, demonstrating that the EGTA-resistant annexin II is a peripheral membrane protein. Nonidet P-40 134-146 annexin A2 Homo sapiens 17-27 9218131-6 1997 This fraction of annexin II was also extracted by 0.1 M Na2CO3, pH 11 and partitioned into the aqueous phase after being treated with Triton X-114, demonstrating that the EGTA-resistant annexin II is a peripheral membrane protein. Nonidet P-40 134-146 annexin A2 Homo sapiens 186-196 9615614-4 1997 The results demonstrate how buffer-diluted n-butanol extracts of 5"-nucleotidase and alkaline phosphatase can be successfully employed for subsequent Triton X-114 fractionation of the enzymes. Nonidet P-40 150-162 5'-nucleotidase ecto Homo sapiens 65-80 9013719-5 1997 This was confirmed by Triton X-114 extractions of post-nuclear supernatants showing that rab4 partitioned into the aqueous phase of Triton X-114, which is indicative of a lack of isoprenylation. Nonidet P-40 22-34 RAB4A, member RAS oncogene family Rattus norvegicus 89-93 9013719-5 1997 This was confirmed by Triton X-114 extractions of post-nuclear supernatants showing that rab4 partitioned into the aqueous phase of Triton X-114, which is indicative of a lack of isoprenylation. Nonidet P-40 132-144 RAB4A, member RAS oncogene family Rattus norvegicus 89-93 8905289-7 1996 Triton X-114 extractions showed that the fetal rab3D partitioned into the detergent phase, suggesting that it was posttranslationally isoprenylated. Nonidet P-40 0-12 RAB3D, member RAS oncogene family Rattus norvegicus 47-52 8810343-5 1996 The A33 protein was purified from Triton X-114 extracts of LIM1215 cells under nondenaturing conditions. Nonidet P-40 34-46 glycoprotein A33 Homo sapiens 4-7 8894530-1 1996 An enzyme-linked immunosorbent assay (ELISA) was developed against Rhodococcus equi using Triton X-114 detergent extracted whole cell material, in which the virulence associated protein (VapA) predominated. Nonidet P-40 90-102 virulence-associated 15-17 kDa antigen Rhodococcus equi 187-191 8794334-4 1996 Complementary biochemical data, such as partitioning into the Triton X-114 detergent phase and stripping of the viral membranes with Nonidet P-40 and dithiothreitol, suggest that p39 is associated with the innermost of the two membranes surrounding the core. Nonidet P-40 62-74 cyclin dependent kinase 5 regulatory subunit 2 Homo sapiens 179-182 8794334-4 1996 Complementary biochemical data, such as partitioning into the Triton X-114 detergent phase and stripping of the viral membranes with Nonidet P-40 and dithiothreitol, suggest that p39 is associated with the innermost of the two membranes surrounding the core. Nonidet P-40 133-145 cyclin dependent kinase 5 regulatory subunit 2 Homo sapiens 179-182 8765099-9 1996 The luminal location of CK II is suggested because CK II was extracted from the SR by l M NaCl only after their treatment with hypotonic medium, and CK II activity was inhibited with the charged inhibitors heparin and polyaspartyl peptide only after their incubation with the SR in the presence of NP-40. Nonidet P-40 298-303 casein kinase 2 alpha 1 Homo sapiens 24-29 8765099-9 1996 The luminal location of CK II is suggested because CK II was extracted from the SR by l M NaCl only after their treatment with hypotonic medium, and CK II activity was inhibited with the charged inhibitors heparin and polyaspartyl peptide only after their incubation with the SR in the presence of NP-40. Nonidet P-40 298-303 casein kinase 2 alpha 1 Homo sapiens 51-56 8765099-9 1996 The luminal location of CK II is suggested because CK II was extracted from the SR by l M NaCl only after their treatment with hypotonic medium, and CK II activity was inhibited with the charged inhibitors heparin and polyaspartyl peptide only after their incubation with the SR in the presence of NP-40. Nonidet P-40 298-303 casein kinase 2 alpha 1 Homo sapiens 51-56 8764987-4 1996 Exemplifying this is the fact that csp immunoreactivity is detected in Triton X-114 extracts of human blood, an observation which may facilitate blood-based diagnostic assays of csp status in man. Nonidet P-40 71-83 DnaJ heat shock protein family (Hsp40) member C5 Homo sapiens 35-38 8648710-7 1996 Triton X-114 partitioning experiments indicated that the A33R gene product is an integral membrane protein. Nonidet P-40 0-12 EEV membrane phosphoglycoprotein Vaccinia virus 57-61 8687440-4 1996 In the Triton X-114 phase separation assay, T beta gamma-MEKA complex was recovered in the aqueous phase, whereas T beta gamma was recover in the detergent phase. Nonidet P-40 7-19 phosducin Homo sapiens 57-61 8888583-7 1996 By isolation with Nonidet P40 and glutaraldehyde, HIV-1 cores were confirmed to consist of p24 protein by immunogold labeling. Nonidet P-40 18-29 transmembrane p24 trafficking protein 2 Homo sapiens 91-94 8738417-10 1996 In contrast, bacterially expressed rab3D partitioned solely into the aqueous phase in Triton X-114 extractions. Nonidet P-40 86-98 RAB3D, member RAS oncogene family Rattus norvegicus 35-40 8796265-6 1996 The released enzyme is a soluble form of CPM as shown by Triton X-114 partitioning, immunoprecipitation, Western blotting, inhibition studies and its neutral pH optimum. Nonidet P-40 57-69 carboxypeptidase M Canis lupus familiaris 41-44 8570098-3 1995 After solubilization with Triton X-114, the ACE-like enzyme contained in the detergent-poor fraction was separated using five steps of purification including gel permeation and anion exchange chromatographies followed by reverse-phase HPLC. Nonidet P-40 26-38 LOW QUALITY PROTEIN: angiotensin-converting enzyme Cavia porcellus 44-47 8724132-7 1996 Similarly, direct use of detergents such as Tween 20, Nonidet P-40, or Triton X-100 as blocking agents also preserved the ASOR-binding activity of RHL1. Nonidet P-40 54-66 asialoglycoprotein receptor 1 Rattus norvegicus 147-151 8726475-8 1996 Peripheral interaction of GCP230 with membranes was confirmed by phase separation in Triton X-114 solution and by extraction with sodium carbonate. Nonidet P-40 85-97 golgin B1 Homo sapiens 26-29 8576121-5 1996 Furthermore, pIgR is the most prominent 35S-labeled CaM-binding protein in the detergent phase of Triton X-114-solubilized, metabolically labeled pIgR-expressing Madin-Darby canine kidney cells. Nonidet P-40 98-110 polymeric immunoglobulin receptor Canis lupus familiaris 13-17 8576121-5 1996 Furthermore, pIgR is the most prominent 35S-labeled CaM-binding protein in the detergent phase of Triton X-114-solubilized, metabolically labeled pIgR-expressing Madin-Darby canine kidney cells. Nonidet P-40 98-110 calmodulin 1 Rattus norvegicus 52-55 8576121-5 1996 Furthermore, pIgR is the most prominent 35S-labeled CaM-binding protein in the detergent phase of Triton X-114-solubilized, metabolically labeled pIgR-expressing Madin-Darby canine kidney cells. Nonidet P-40 98-110 polymeric immunoglobulin receptor Canis lupus familiaris 146-150 8820970-6 1996 The amphiphilic properties of the several AChE and BuChE molecules were analyzed by Triton X-114 phase-partitioning and by phenyl-agarose chromatography. Nonidet P-40 84-96 acetylcholinesterase Mus musculus 42-46 8820970-8 1996 The G4A AChE and BuChE isoforms differed in their interaction with Triton X-114 and with a hydrophobic matrix. Nonidet P-40 67-79 acetylcholinesterase Mus musculus 8-12 8666426-7 1996 Sequential treatment with saponin and Nonidet P-40 was used to fractionate tyrosine-phosphorylated proteins during Thy-1-mediated activation of RBL-2H3 cells. Nonidet P-40 38-50 Thy-1 cell surface antigen Rattus norvegicus 115-120 7499868-7 1995 Various detergents (Nonidet P-40, digitonin, lubrol, and Triton X-114) were used to solubilize membrane C1q. Nonidet P-40 20-32 complement C1q A chain Homo sapiens 104-107 7499868-7 1995 Various detergents (Nonidet P-40, digitonin, lubrol, and Triton X-114) were used to solubilize membrane C1q. Nonidet P-40 57-69 complement C1q A chain Homo sapiens 104-107 7588748-2 1995 In microsomes, temperature-induced phase separation using Triton X-114 elucidated the partition of BSDL between the aqueous phase and the detergent-rich phase containing hydrophilic and membrane proteins, respectively. Nonidet P-40 58-70 carboxyl ester lipase Homo sapiens 99-103 8821178-3 1996 GAP-43 (Ala41) was significantly more extractable with Nonidet P-40 and less tightly associated with the membrane skeleton than the wild-type protein. Nonidet P-40 55-67 growth associated protein 43 Rattus norvegicus 0-6 7479877-5 1995 Treatment with phosphatidylinositol-specific phospholipase C shifted Mkc7 activity from the detergent to the aqueous phase in a Triton X-114 phase separation, indicating that membrane attachment of Mkc7 is mediated by a glycosyl-phosphatidylinositol anchor. Nonidet P-40 128-140 aspartyl protease Saccharomyces cerevisiae S288C 69-73 7479877-5 1995 Treatment with phosphatidylinositol-specific phospholipase C shifted Mkc7 activity from the detergent to the aqueous phase in a Triton X-114 phase separation, indicating that membrane attachment of Mkc7 is mediated by a glycosyl-phosphatidylinositol anchor. Nonidet P-40 128-140 aspartyl protease Saccharomyces cerevisiae S288C 198-202 7478260-2 1995 The amphiphilic or hydrophilic behavior of the AChE and BuChE forms was assessed by sedimentation analysis, hydrophobic chromatography and Triton X-114 phase-partitioning. Nonidet P-40 139-151 acetylcholinesterase (Cartwright blood group) Homo sapiens 47-51 7629168-8 1995 Part of the Grb2 complex associated with the particulate compartment could be extracted with Nonidet P-40, while the rest was Nonidet P-40 insoluble. Nonidet P-40 93-105 growth factor receptor bound protein 2 Homo sapiens 12-16 7629168-8 1995 Part of the Grb2 complex associated with the particulate compartment could be extracted with Nonidet P-40, while the rest was Nonidet P-40 insoluble. Nonidet P-40 126-138 growth factor receptor bound protein 2 Homo sapiens 12-16 7478260-2 1995 The amphiphilic or hydrophilic behavior of the AChE and BuChE forms was assessed by sedimentation analysis, hydrophobic chromatography and Triton X-114 phase-partitioning. Nonidet P-40 139-151 butyrylcholinesterase Homo sapiens 56-61 7772046-7 1995 We demonstrate that following temperature-induced phase separation in Triton X-114, the membrane-associated annexin V partitions predominantly into the aqueous phase. Nonidet P-40 70-82 annexin A5 Homo sapiens 108-117 7638087-5 1995 After PIPLC treatment, the enzymes were recovered in the aqueous phase after phase repartition in Triton X-114 indicating that PIPLC removed the hydrophobic domain converting the enzymes from membrane-linked to aqueous soluble forms. Nonidet P-40 98-110 phospholipase C beta 1 Homo sapiens 6-11 7638087-5 1995 After PIPLC treatment, the enzymes were recovered in the aqueous phase after phase repartition in Triton X-114 indicating that PIPLC removed the hydrophobic domain converting the enzymes from membrane-linked to aqueous soluble forms. Nonidet P-40 98-110 phospholipase C beta 1 Homo sapiens 127-132 8537321-4 1995 (i) Treatment of TNF-alpha with 1% NP40 enhanced the dissociation from the trimer to the monomer. Nonidet P-40 35-39 tumor necrosis factor Homo sapiens 17-26 10846541-3 1995 In addition, a study of the different CEA molecular forms separated by Triton X114 partitioning, immunoprecipitation and immunoblotting was completed. Nonidet P-40 71-82 CEA cell adhesion molecule 3 Homo sapiens 38-41 7787299-2 1995 PAS-4 was selectively extracted with Triton X-114 nonionic detergent and then fractionated on DEAE-Sepharose at pH 7.5. Nonidet P-40 37-49 PAS-4 Bos taurus 0-5 7543624-5 1995 Using a Triton X-114 phase separation method 91 to 97% of urinary CD59 was found to be in a soluble form without anchor-associated phospholipid. Nonidet P-40 8-20 CD59 molecule (CD59 blood group) Homo sapiens 66-70 7525568-7 1994 This association of Syk with components of the antigen receptor complex was stable to Nonidet P-40. Nonidet P-40 86-98 spleen associated tyrosine kinase Homo sapiens 20-23 7836456-4 1995 Neuronal GAD67 is partially recovered with GAD65 in membrane-containing pellet fractions and Triton X-114 detergent phases. Nonidet P-40 93-105 glutamate decarboxylase 1 Homo sapiens 9-14 7704843-12 1995 Immunization of mice with the lipid-modified VapA purified by SDS-PAGE fractionation or with acetone precipitated VapA protein following TX-114 extraction resulted in significantly enhanced clearance from the liver and spleen following intravenous challenge. Nonidet P-40 137-143 vesicle-associated membrane protein, associated protein A Mus musculus 45-49 7704843-12 1995 Immunization of mice with the lipid-modified VapA purified by SDS-PAGE fractionation or with acetone precipitated VapA protein following TX-114 extraction resulted in significantly enhanced clearance from the liver and spleen following intravenous challenge. Nonidet P-40 137-143 vesicle-associated membrane protein, associated protein A Mus musculus 114-118 7533539-3 1995 All cationic PKC substrates tested, neurogranin peptide analog, neurogranin, and histone III-S, formed aggregates with PS/DG/NP-40/Ca2+ mixed micelles in a time-dependent fashion. Nonidet P-40 125-130 protein kinase C alpha Homo sapiens 13-16 7533539-3 1995 All cationic PKC substrates tested, neurogranin peptide analog, neurogranin, and histone III-S, formed aggregates with PS/DG/NP-40/Ca2+ mixed micelles in a time-dependent fashion. Nonidet P-40 125-130 neurogranin Homo sapiens 36-47 7533539-3 1995 All cationic PKC substrates tested, neurogranin peptide analog, neurogranin, and histone III-S, formed aggregates with PS/DG/NP-40/Ca2+ mixed micelles in a time-dependent fashion. Nonidet P-40 125-130 neurogranin Homo sapiens 64-75 8068012-1 1994 The membrane receptor for retinol-binding protein (RBP) has been solubilized from human placental brush-border membranes with octyl-beta-glucoside, Nonidet P-40 and CHAPS. Nonidet P-40 148-160 retinol binding protein 4 Homo sapiens 26-49 7861396-1 1994 The seminal plasma complement regulator membrane cofactor protein (MCP) was examined by sequential centrifugation and phase separation in the detergent Triton X-114. Nonidet P-40 152-164 CD46 molecule Homo sapiens 40-65 7861396-1 1994 The seminal plasma complement regulator membrane cofactor protein (MCP) was examined by sequential centrifugation and phase separation in the detergent Triton X-114. Nonidet P-40 152-164 CD46 molecule Homo sapiens 67-70 7861396-9 1994 The 40 kDa sperm MCP protein was absent but a 60 kDa MCP component, which partitioned to the detergent phase in Triton X-114, was evident. Nonidet P-40 112-124 CD46 molecule Homo sapiens 53-56 8068012-1 1994 The membrane receptor for retinol-binding protein (RBP) has been solubilized from human placental brush-border membranes with octyl-beta-glucoside, Nonidet P-40 and CHAPS. Nonidet P-40 148-160 retinol binding protein 4 Homo sapiens 51-54 8068012-4 1994 The detergent-solubilized form retained all the properties characteristic of the membrane-bound protein, except for a small decrease in affinity for RBP (3- and 7-fold in Nonidet P-40 and octyl-beta-glucoside respectively). Nonidet P-40 171-183 retinol binding protein 4 Homo sapiens 149-152 8070406-4 1994 These proteins coprecipitate with mIgM in Triton X-100 and Nonidet P-40, but not in digitonin lysates. Nonidet P-40 59-71 immunoglobulin heavy constant mu Mus musculus 34-38 8037672-3 1994 In order to identify the biosynthetic origin of PI-PLC resistance we determined the PI-PLC sensitivity of AP in 35S-labelled cells (10 min pulse; 0-60 min chase) by Triton X-114 phase separation. Nonidet P-40 165-177 phospholipase C beta 1 Homo sapiens 84-90 8207008-7 1994 Upon treatment of post-Golgi membranes with urea or Triton X-114, a portion of the bound alpha B-crystallin remains tightly associated, indicating that the alpha B-form may mediate membrane binding of an alpha-crystallin multimeric complex. Nonidet P-40 52-64 crystallin alpha B Bos taurus 89-107 7584087-4 1994 Simple and effective methods of removing the contaminating LPS using either a polymyxin B resin or Triton X-114 extraction are described. Nonidet P-40 99-111 interferon regulatory factor 6 Homo sapiens 59-62 8014005-6 1994 By phase separation using Triton X-114, CEA in the cell lysates of crypts was separated mostly into the detergent phase, while the spontaneously released and the PI-PLC-cleaved CEA were separated into the aqueous phase. Nonidet P-40 26-38 CEA cell adhesion molecule 3 Homo sapiens 40-43 8195152-6 1994 When either native plasma membrane-associated or in vitro-transcribed and -translated SBP were subjected to Triton X-114 phase separation, 80% partitioned into the detergent-poor aqueous phase. Nonidet P-40 108-120 sucrose-binding protein Glycine max 86-89 8279519-2 1993 Extraction of the membranes with the nonionic detergents Nonidet P-40 and Triton X-100, followed by centrifugation at 100,000 g, resulted in the doubling of the IP3 receptor in the pellets, whereas no detectable binding was found in the supernatants. Nonidet P-40 57-69 inositol 1,4,5-trisphosphate receptor type 3 Homo sapiens 161-173 8175672-8 1994 Repeated washing of the membrane fraction followed by extraction with Triton X-114 suggested that both endopeptidase 24.15 and prolyl endopeptidase, although predominantly soluble, can be peripherally associated with membranes. Nonidet P-40 70-82 thimet oligopeptidase 1 Rattus norvegicus 103-122 8175672-8 1994 Repeated washing of the membrane fraction followed by extraction with Triton X-114 suggested that both endopeptidase 24.15 and prolyl endopeptidase, although predominantly soluble, can be peripherally associated with membranes. Nonidet P-40 70-82 prolyl endopeptidase Rattus norvegicus 127-147 8300636-4 1994 In this study, sensitivity to bacterial phosphatidylinositol-specific phospholipase C, biosynthetic labeling with [3H]ethanolamine, and partitioning in Triton X-114 are used to establish that p97 is expressed at the cell surface as a glycosylphosphatidylinositol-anchored protein. Nonidet P-40 152-164 melanotransferrin Homo sapiens 192-195 8144671-2 1994 Using Triton X-114 phase separation analysis and charge-shift electrophoresis, this study demonstrates that CPE exhibits the amphiphilicity required for membrane insertion, but this behavior develops only after exposure of CPE to membranes. Nonidet P-40 6-18 cpe Clostridium perfringens 108-111 8411260-8 1993 Triton X-114 (TX-114) detergent extracts from cells cultured on collagen I or plastic were incubated with latent MMP-2 and analyzed by zymography to localize the MMP-2 activator. Nonidet P-40 0-12 matrix metallopeptidase 2 Homo sapiens 113-118 8411260-8 1993 Triton X-114 (TX-114) detergent extracts from cells cultured on collagen I or plastic were incubated with latent MMP-2 and analyzed by zymography to localize the MMP-2 activator. Nonidet P-40 0-12 matrix metallopeptidase 2 Homo sapiens 162-167 8308062-7 1993 Consistent with these results, rab6 antigen, originally found as 40% in the cytosolic versus 60% in the particulate (P 150,000 g) fraction, became almost entirely cytosolic; moreover, it partitioned in the aqueous phase of Triton X-114 whereas the membrane fraction was detergent-soluble. Nonidet P-40 223-235 RAB6A, member RAS oncogene family Homo sapiens 31-35 8411269-7 1993 Eighty and 45% of the AChE and BuChE activities in S2 were measured in the detergent-rich phase by Triton X-114 phase partitioning. Nonidet P-40 99-111 acetylcholinesterase (Cartwright blood group) Homo sapiens 22-26 7903200-4 1993 Nonionic detergents such as Triton X-100 or Nonidet P-40 at very low concentrations were found to completely abolish azidopine photolabeling to P-glycoprotein and are able to reverse the multidrug resistance phenotype. Nonidet P-40 44-56 ATP binding cassette subfamily B member 1 Homo sapiens 144-158 8240242-9 1993 Phase partitioning in Triton X-114 indicated that MP50 is a peripheral membrane protein. Nonidet P-40 22-34 glutamate dehydrogenase 1, mitochondrial Sus scrofa 50-54 8215392-3 1993 The total CEA content of LS-174T cells, quantitated by Triton X-114 extraction followed by radioimmunoassay or by immunohistochemistry, was 3.5-fold higher than that of other cells (P < 0.001). Nonidet P-40 55-67 CEA cell adhesion molecule 3 Homo sapiens 10-13 8425534-0 1993 Differential insertion of insulin receptor complexes into Triton X-114 bilayer membranes. Nonidet P-40 58-70 insulin receptor Homo sapiens 26-42 8428954-9 1993 We first identified outer membrane Cyt b as a tightly bound, Triton X-114-extractable, 23-kDa polypeptide. Nonidet P-40 61-73 cytochrome b, mitochondrial Rattus norvegicus 35-40 8428965-14 1993 CBP/tk binding activity in nuclear extracts was abolished by heat (60 degrees C, 5 min) or treatment with proteinase K (0.1 microgram/ml) and sodium dodecyl sulfate (0.005%), but not by Nonidet P-40 or Triton X-100. Nonidet P-40 186-198 CREB binding protein Homo sapiens 0-3 8425534-4 1993 Upon raising the temperature of a micellar Triton X-114 solution above the cloud-point, a detergent enriched phase pellets and coprecipitates 95% of the purified insulin-free (alpha beta)2 receptors. Nonidet P-40 43-55 insulin Homo sapiens 162-169 1522116-4 1992 In this paper we show that LSP1 protein which is immunoprecipitated with anti-LSP1 antibodies from NP-40-soluble lysates of the mouse B-lymphoma cell line BAL17 is associated with actin. Nonidet P-40 99-104 lymphocyte specific 1 Mus musculus 27-31 1416964-6 1992 Furthermore, the phase separation experiments with Triton X-114 provide the first evidence showing that the mature form of cathepsin L polypeptide is strongly associated with the vacuolar membranes. Nonidet P-40 51-63 cathepsin L Mus musculus 123-134 1522116-4 1992 In this paper we show that LSP1 protein which is immunoprecipitated with anti-LSP1 antibodies from NP-40-soluble lysates of the mouse B-lymphoma cell line BAL17 is associated with actin. Nonidet P-40 99-104 lymphocyte specific 1 Mus musculus 78-82 1321338-7 1992 The binding of HSF activated in vitro by Nonidet P-40 was not suppressed by the addition of quercetin. Nonidet P-40 41-53 interleukin 6 Homo sapiens 15-18 1573391-7 1992 Triton X-114 phase partitioning and alkaline extraction of synaptic membranes indicate that both gp65 and gp55 are integral membrane glycoproteins. Nonidet P-40 0-12 neuroplastin Homo sapiens 97-101 1591009-6 1992 Furthermore, Triton X-114 extractions from CHO cells revealed a modification of the SP-C precursor. Nonidet P-40 13-25 surfactant protein C Homo sapiens 84-88 1573391-7 1992 Triton X-114 phase partitioning and alkaline extraction of synaptic membranes indicate that both gp65 and gp55 are integral membrane glycoproteins. Nonidet P-40 0-12 neuroplastin Homo sapiens 106-110 1582408-7 1992 Extraction experiments and protease protection experiments indicated that p85 is an integral membrane protein although it partitioned atypically during Triton X-114 phase separation. Nonidet P-40 152-164 phosphoinositide-3-kinase regulatory subunit 2 Homo sapiens 74-77 1572102-2 1992 F2 was also extracted from the cells by 60% ammonium sulphate, 0.5% CHAPS and 0.28% Triton X-114. Nonidet P-40 84-96 transcription termination factor 2 Homo sapiens 0-2 1583718-4 1992 gp41 was efficiently galactosylated by galactosyltransferase only in the presence of Nonidet P-40, suggesting that GlcNAc residues are not exposed on the surface of the virion. Nonidet P-40 85-97 occlusion-derived virus glycoprotein Autographa californica nucleopolyhedrovirus 0-4 1583718-4 1992 gp41 was efficiently galactosylated by galactosyltransferase only in the presence of Nonidet P-40, suggesting that GlcNAc residues are not exposed on the surface of the virion. Nonidet P-40 85-97 probable glycosyltransferase 2 Triticum aestivum 39-60 1591355-1 1992 The interaction of the human acrosomal protein SP-10 with the acrosomal membranes was analyzed by the ability of Triton X-114 (TX-114) and other agents to release SP-10 from the acrosome. Nonidet P-40 113-125 acrosomal vesicle protein 1 Homo sapiens 29-52 1591355-1 1992 The interaction of the human acrosomal protein SP-10 with the acrosomal membranes was analyzed by the ability of Triton X-114 (TX-114) and other agents to release SP-10 from the acrosome. Nonidet P-40 113-125 acrosomal vesicle protein 1 Homo sapiens 47-52 1591355-1 1992 The interaction of the human acrosomal protein SP-10 with the acrosomal membranes was analyzed by the ability of Triton X-114 (TX-114) and other agents to release SP-10 from the acrosome. Nonidet P-40 127-133 acrosomal vesicle protein 1 Homo sapiens 29-52 1591355-1 1992 The interaction of the human acrosomal protein SP-10 with the acrosomal membranes was analyzed by the ability of Triton X-114 (TX-114) and other agents to release SP-10 from the acrosome. Nonidet P-40 127-133 acrosomal vesicle protein 1 Homo sapiens 47-52 1591355-2 1992 Treatment of human sperm with TX-114 revealed a pool of SP-10 that was released by TX-114 and a pool of SP-10 that was TX-114-resistant. Nonidet P-40 30-36 acrosomal vesicle protein 1 Homo sapiens 56-61 1591355-2 1992 Treatment of human sperm with TX-114 revealed a pool of SP-10 that was released by TX-114 and a pool of SP-10 that was TX-114-resistant. Nonidet P-40 30-36 acrosomal vesicle protein 1 Homo sapiens 104-109 1591355-2 1992 Treatment of human sperm with TX-114 revealed a pool of SP-10 that was released by TX-114 and a pool of SP-10 that was TX-114-resistant. Nonidet P-40 83-89 acrosomal vesicle protein 1 Homo sapiens 56-61 1591355-2 1992 Treatment of human sperm with TX-114 revealed a pool of SP-10 that was released by TX-114 and a pool of SP-10 that was TX-114-resistant. Nonidet P-40 83-89 acrosomal vesicle protein 1 Homo sapiens 56-61 1591355-3 1992 TX-114-resistant SP-10 was associated with the equatorial segment and with TX-114-resistant portions of the acrosomal matrix and the inner acrosomal membrane. Nonidet P-40 0-6 acrosomal vesicle protein 1 Homo sapiens 17-22 1591355-3 1992 TX-114-resistant SP-10 was associated with the equatorial segment and with TX-114-resistant portions of the acrosomal matrix and the inner acrosomal membrane. Nonidet P-40 75-81 acrosomal vesicle protein 1 Homo sapiens 17-22 1591355-4 1992 Phase partitioning of TX-114-released and TX-114-resistant SP-10 pools showed that both were hydrophilic, indicating that these pools consist of proteins that are peripherally associated with, rather than integral to, the acrosomal membranes. Nonidet P-40 42-48 acrosomal vesicle protein 1 Homo sapiens 59-64 1591355-6 1992 Together the results suggest that SP-10 is a hydrophilic peripheral acrosomal membrane protein that may be associated with a TX-114-resistant "anchor." Nonidet P-40 125-131 acrosomal vesicle protein 1 Homo sapiens 34-39 1371790-6 1992 Although Leu 8 mAb immunoprecipitated only a single protein of approximately 80 kDa from T cell lysates treated with Nonidet P-40 under reducing condition, it coimmunoprecipitated additional proteins of 48, 42, 28, 24, and 22 kDa from T cell lysates treated with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate. Nonidet P-40 117-129 selectin L Homo sapiens 9-14 1560018-2 1992 Optimum enzyme activity for the deacylation of PLP was obtained in 0.5% Triton X-100, 1 mM dithiothreitol at pH 7.0 and at 37 degrees C. Other detergents (octyl beta-D-glucoside, Nonidet P-40, and Tween 20) have little or no effect, whereas deacylation was completely abolished by 0.1% sodium dodecyl sulfate or boiling the membrane fraction for 5 min prior to incubation. Nonidet P-40 179-191 proteolipid protein 1 Rattus norvegicus 47-50 1548059-6 1992 The mutated protein D molecule was not acylated and partitioned in the aqueous phase after Triton X-114 extraction of intact bacteria, unlike native and recombinant protein D, which partitioned in the detergent phase. Nonidet P-40 91-103 protein D Escherichia coli 12-21 1764034-5 1991 Fractionation of the intracellular forms of CPE with Triton X-114 at various pH values indicates that the membrane-bound, but not the soluble, form is amphipathic; this difference probably arises from post-translational modification of the enzyme. Nonidet P-40 53-65 carboxypeptidase E Homo sapiens 44-47 1740454-5 1992 The surface CgB was not released by exposure to pH 11, yet it partitioned in the aqueous phase upon Triton X-114 phase separation. Nonidet P-40 100-112 chromogranin B Rattus norvegicus 12-15 1626415-6 1992 Triton X-114 extraction and dimethyl suberimidate-HCl crosslinking indicate that the p11 Z protein is a hydrophobic protein associated with the nucleocapsid of the virion core. Nonidet P-40 0-12 endonuclease, poly(U) specific Homo sapiens 85-88 1835378-4 1991 However, when membranes were treated with GPI-PLD in the presence of 0.1% Nonidet P-40 substantial GPI anchor degradation (as measured by Triton X-114 phase separation) was observed. Nonidet P-40 74-86 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 42-49 1918067-18 1991 Triton X-114 partitioning of the integral membrane proteins of rough microsomes suggested that pgp35 (SSR alpha) and calnexin were major Ca(2+)-binding proteins of the endoplasmic reticulum membrane. Nonidet P-40 0-12 signal sequence receptor subunit 1 Homo sapiens 102-111 1918067-18 1991 Triton X-114 partitioning of the integral membrane proteins of rough microsomes suggested that pgp35 (SSR alpha) and calnexin were major Ca(2+)-binding proteins of the endoplasmic reticulum membrane. Nonidet P-40 0-12 calnexin Homo sapiens 117-125 1835378-4 1991 However, when membranes were treated with GPI-PLD in the presence of 0.1% Nonidet P-40 substantial GPI anchor degradation (as measured by Triton X-114 phase separation) was observed. Nonidet P-40 138-150 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 42-49 1835378-6 1991 As with the cell membranes the reconstituted substrates exhibited marked resistance to the action of purified GPI-PLD which could be overcome by the inclusion of Nonidet P-40. Nonidet P-40 162-174 glycosylphosphatidylinositol specific phospholipase D1 Homo sapiens 110-117 1710649-3 1991 Using the Triton X-114 phase separation system, authentic and recombinant E proteins as well as the gp100 precursor exhibited hydrophobic properties similar to those of integral membrane proteins. Nonidet P-40 10-22 premelanosome protein Homo sapiens 100-105 1710185-5 1991 Solubilization of thylakoid membranes with Triton X-100 and phase partitioning with Triton X-114 indicate an intrinsic localization of HSP 22 or, alternatively, a non-covalent association with integral membrane protein(s). Nonidet P-40 84-96 heat shock protein family B (small) member 8 Homo sapiens 135-141 1655032-3 1991 (2) Subsequent modification of the MTAS-treated microsomes with Triton X-114 reveals that glucose-6-phosphatase assayed at 37 degrees C as well as at 0 degrees C is inhibited. Nonidet P-40 64-76 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 90-111 1710649-7 1991 Using the Triton X-114 phase separation system, the oligomeric form of NS1 was shown to be associated with cellular membranes although it appeared less hydrophobic than protein E and gp100. Nonidet P-40 10-22 influenza virus NS1A binding protein Homo sapiens 71-74 2031716-8 1991 Erythrocyte cell membranes showed acetylcholinesterase activity present as a major form, which was hydrophobic by Triton X-114 phase separation and in nondenaturing gel electrophoresis moved at the same rate as the purified enzyme. Nonidet P-40 114-126 acetylcholinesterase Bos taurus 34-54 1903539-7 1991 Further, 2-mercaptoethanol acts synergistically with deoxycholate plus Nonidet P-40 in converting inactive cytosolic NF-kappa B to an active DNA-binding form. Nonidet P-40 71-83 nuclear factor kappa B subunit 1 Homo sapiens 117-127 1851759-6 1991 In addition, the 150- and 180-kDa TGF-beta-binding proteins are present in the detergent-rich phase during Triton X-114 phase separation, whereas the glomerular TGF-beta-binding proteins partition exclusively into the detergent-poor phase. Nonidet P-40 107-119 transforming growth factor beta 1 Homo sapiens 34-42 1824861-9 1991 p36 appeared to be a peripherally associated granule membrane protein in that it was dissociated from the membrane by addition of base and it partitioned with the aqueous phase when granule membranes were treated with Triton X-114. Nonidet P-40 218-230 annexin A2 Bos taurus 0-3 2011134-2 1991 Since recombinant interleukin 2 is not secreted by bacteria, but is instead obtained from bacterial lysates, we have analysed several different recombinant interleukin 2 molecules as well as the naturally synthesized cytokine by phase separation in Triton X-114. Nonidet P-40 249-261 interleukin 2 Homo sapiens 18-31 1671640-8 1991 Results from Triton X-114 partitioning experiments suggest that luminal GGT probably exists in both membrane bound and nonmembrane bound forms. Nonidet P-40 13-25 gamma-glutamyltransferase 1 Rattus norvegicus 72-75 2249987-6 1990 In transfected cell extracts, THP also remained primarily in the detergent phase in a Triton X-114 partitioning assay, indicating that it has a hydrophobic character, in contrast to its behavior after isolation from human urine. Nonidet P-40 86-98 uromodulin Homo sapiens 30-33 1846040-3 1991 The rap GAP activity remained quantitatively associated with the membrane following washes with buffered 1 M LiCl containing 20 mM EDTA but was solubilized with the detergents Nonidet P-40 and deoxycholate. Nonidet P-40 176-188 LDL receptor related protein associated protein 1 Homo sapiens 4-7 2249987-7 1990 Triton X-114 detergent-associated THP was redistributed to the aqueous phase after treatment of cell extracts with phosphatidylinositol-specific phospholipase C. Treatment of intact transfected HeLa cells with phosphatidylinositol-specific phospholipase C also resulted in the release of THP into the medium, suggesting that it is a glycosylphosphatidylinositol (GPI)-linked membrane protein. Nonidet P-40 0-12 uromodulin Homo sapiens 34-37 2249987-7 1990 Triton X-114 detergent-associated THP was redistributed to the aqueous phase after treatment of cell extracts with phosphatidylinositol-specific phospholipase C. Treatment of intact transfected HeLa cells with phosphatidylinositol-specific phospholipase C also resulted in the release of THP into the medium, suggesting that it is a glycosylphosphatidylinositol (GPI)-linked membrane protein. Nonidet P-40 0-12 uromodulin Homo sapiens 288-291 1965942-3 1990 Purified 5"-nucleotidase was treated with PIPLC and the resultant enzyme was almost totally partitioned into the detergent-poor phase following phase-separation in Triton X-114 indicating that PIPLC converted the enzyme from an amphipathic to a hydrophilic form. Nonidet P-40 164-176 5' nucleotidase, ecto Rattus norvegicus 9-24 2141610-10 1990 In addition, annexin X partitioned into the detergent phase of Triton X-114 as a function of calcium. Nonidet P-40 63-75 Annexin B10 Drosophila melanogaster 13-22 2269658-7 1990 Carbonate (pH = 11) washing and Triton X-114 cloud-point precipitations of yeast microsomes indicated that ribophorin I was integrated into the membrane bilayer. Nonidet P-40 32-44 ribophorin I Homo sapiens 107-119 2246332-1 1990 A Nonidet P-40 extract of growth hormone-producing rat pituitary MtT/S cells was found to contain peptidylarginine deiminase (EC 3.5.3.15), which was indistinguishable from an enzyme preparation from rat muscle in Western immunoblotting and immunoprecipitation. Nonidet P-40 2-14 gonadotropin releasing hormone receptor Rattus norvegicus 26-40 1703989-2 1990 Purified DAF and Factor H, in 0.1% NP-40, were assayed by measuring the amount required to release 50% of the radiolabelled Bb in 10 min from C3b,Bb on Zym or cross-linked erythrocytes. Nonidet P-40 35-40 complement decay-accelerating factor Bos taurus 9-12 1979722-5 1990 Triton X-114 phase-partition experiments showed that secondary granule fraction CD15 antigens could be partitioned into hydrophilic (aqueous phase) and hydrophobic (detergent phase) antigens, suggesting that several of these antigens were integral secondary granule membrane components. Nonidet P-40 0-12 fucosyltransferase 4 Homo sapiens 80-84 2076702-7 1990 In addition, GP-2 in the pancreatic juice was recovered in the aqueous phase during Triton X-114 extraction and yet remained sedimentable after detergent extraction, demonstrating that its ability to remain in large aggregates was independent of lipid. Nonidet P-40 84-96 glycoprotein 2 Rattus norvegicus 13-17 2373740-4 1990 Partitioning in Triton X-114 showed that C-CAM has hydrophobic properties. Nonidet P-40 16-28 CEA cell adhesion molecule 1 Rattus norvegicus 41-46 2378890-2 1990 The hydrophobic properties of AChE forms were studied by phase-partition of Triton X-114 and Triton X-100-solubilized enzyme and by comparing their migration in sucrose gradient containing either Triton X-100 or Brij 96. Nonidet P-40 76-88 ACE-1 Oryctolagus cuniculus 30-34 2303412-6 1990 Both the 21-residue COOH-terminal peptide and the purified membrane form of CPE, but not the soluble form, partition into Triton X-114 only at low pH (pH less than 6). Nonidet P-40 122-134 carboxypeptidase E Bos taurus 76-79 2182079-5 1990 Extraction of cells with the nonionic detergent Triton X-114 identified plasminogen activator and plasmin associated with the cell surface. Nonidet P-40 48-60 plasminogen Homo sapiens 72-79 1969407-7 1990 TH can be removed from granule membranes by the detergents Nonidet P-40, Triton X-100, and 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate. Nonidet P-40 59-71 tyrosine hydroxylase Bos taurus 0-2 1969407-9 1990 Fractionation of granule membranes by temperature-induced phase separation in Triton X-114 revealed that TH is recovered in phases in which integral (detergent phase) and hydrophobic (phospholipid phase) membrane proteins are typically found. Nonidet P-40 78-90 tyrosine hydroxylase Bos taurus 105-107 2809601-4 1989 GP50 remained associated with the membrane fraction following extraction of SMs with alkaline sodium carbonate, was partially (55%) present in the detergent phase following the phase partitioning of SMs in the presence of Triton X-114, and was resistant to proteolytic digestion with trypsin when present as a component of intact membranes. Nonidet P-40 222-234 tumor associated calcium signal transducer 2 Homo sapiens 0-4 33808741-7 2021 We showed by immunoblotting that the significant part of T-cadherin was detected in specific membrane domains (detergent Triton X-114 resistant) and the molecular weight of this newly identified protein was greater than that of T-cadherin from nucleated cells. Nonidet P-40 121-133 cadherin 13 Homo sapiens 57-67 2499539-3 1989 Binding of the nonionic detergent Triton X-114 by mutant ETA occurred at a slightly higher pH than did binding by wild-type ETA, suggesting that the mutant protein more readily undergoes a conformational change exposing hydrophobic regions. Nonidet P-40 34-46 endothelin receptor type A Mus musculus 57-60 2679882-4 1989 Adipsin does not appear to be anchored by a glycosylphosphatidylinositol moiety, since adipsin which was been released with Triton X-114 from an intracellular membrane fraction partitions into the aqueous phase. Nonidet P-40 124-136 complement factor D Homo sapiens 87-94 2526822-1 1989 Extracts from myelinated and unmyelinated nerves, prepared using Nonidet P-40, contained receptors for C3b/C4b (CR1). Nonidet P-40 65-77 complement C3 Homo sapiens 103-106 2526822-1 1989 Extracts from myelinated and unmyelinated nerves, prepared using Nonidet P-40, contained receptors for C3b/C4b (CR1). Nonidet P-40 65-77 complement C4B (Chido blood group) Homo sapiens 107-110 2499539-3 1989 Binding of the nonionic detergent Triton X-114 by mutant ETA occurred at a slightly higher pH than did binding by wild-type ETA, suggesting that the mutant protein more readily undergoes a conformational change exposing hydrophobic regions. Nonidet P-40 34-46 endothelin receptor type A Mus musculus 124-127 2523178-8 1989 All three glycoproteins released from JE-infected Vero cells were associated with extracellular particles, the virion in the case of E and a low density particle in the case of and NS1" exhibited amphipathic properties in Triton X-114 extraction experiments. Nonidet P-40 222-234 influenza virus NS1A binding protein Homo sapiens 181-184 2730918-5 1989 Sephadex G-200 gel-permeation chromatography in the presence of 0.1% Nonidet P-40 indicated that the native-state molecular masses of microsomal biotinidase and lipoamidase were 68 and 120 kDa, respectively. Nonidet P-40 69-81 biotinidase Cavia porcellus 145-156 2525840-3 1989 Newly synthesized monomeric NS1 is a hydrophilic and water-soluble protein which cannot be pelleted at 75,000 g. After dimerization, however, NS1 showed increased hydrophobicity in a Triton X-114 phase partitioning system and was completely pelletable at 75,000 g; these findings are consistent with NS1 becoming membrane-associated. Nonidet P-40 183-195 influenza virus NS1A binding protein Homo sapiens 28-31 2525840-3 1989 Newly synthesized monomeric NS1 is a hydrophilic and water-soluble protein which cannot be pelleted at 75,000 g. After dimerization, however, NS1 showed increased hydrophobicity in a Triton X-114 phase partitioning system and was completely pelletable at 75,000 g; these findings are consistent with NS1 becoming membrane-associated. Nonidet P-40 183-195 influenza virus NS1A binding protein Homo sapiens 142-145 2525840-3 1989 Newly synthesized monomeric NS1 is a hydrophilic and water-soluble protein which cannot be pelleted at 75,000 g. After dimerization, however, NS1 showed increased hydrophobicity in a Triton X-114 phase partitioning system and was completely pelletable at 75,000 g; these findings are consistent with NS1 becoming membrane-associated. Nonidet P-40 183-195 influenza virus NS1A binding protein Homo sapiens 142-145 2926476-4 1989 The major phosphoproteins pp80ac, pp46, and pp40 (Katz et al., 1985), as well as p34 partition into the oil phase of Triton X-114 extracts, suggesting that they are integral membrane proteins, at least in our experimental conditions. Nonidet P-40 117-129 alpha- and gamma-adaptin binding protein Rattus norvegicus 81-84 2918027-4 1989 Membrane-washing experiments indicate that mature GAP-43 is tightly bound to growth cone membranes, and partitioning of Triton X-114-solubilized GAP-43 between detergent-enriched and detergent-depleted phases indicates considerable hydrophobicity. Nonidet P-40 120-132 growth associated protein 43 Rattus norvegicus 145-151 2914931-9 1989 There was also evidence for a Ca2+-dependent protein-protein interaction (aggregation) of PSP present in a Nonidet P-40 extract of cell membranes. Nonidet P-40 107-119 phosphoserine phosphatase Bos taurus 90-93 2536017-4 1989 When cells solubilized in Triton X-114 are subjected to heat-induced phase separation, unoccupied receptor, receptor-bound 125I-uPA, and cross-linked 125I-uPA-receptor complex all partition in the detergent phase, whereas the unbound ligand remains in the aqueous phase; similar phase partitioning is observed with endogenous uPA-receptor complexes from cultured human and murine cells. Nonidet P-40 26-38 plasminogen activator, urokinase Homo sapiens 155-158 2747923-1 1989 Immunohistochemical screening of monoclonal antibodies raised against Triton X-114-treated synaptic membranes revealed two monoclonal antibodies, namely BM88 and BM72, with characteristic binding specificities in the central and peripheral nervous systems of the pig. Nonidet P-40 70-82 cell cycle exit and neuronal differentiation 1 Sus scrofa 153-157 2536017-4 1989 When cells solubilized in Triton X-114 are subjected to heat-induced phase separation, unoccupied receptor, receptor-bound 125I-uPA, and cross-linked 125I-uPA-receptor complex all partition in the detergent phase, whereas the unbound ligand remains in the aqueous phase; similar phase partitioning is observed with endogenous uPA-receptor complexes from cultured human and murine cells. Nonidet P-40 26-38 plasminogen activator, urokinase Homo sapiens 128-131 2536017-4 1989 When cells solubilized in Triton X-114 are subjected to heat-induced phase separation, unoccupied receptor, receptor-bound 125I-uPA, and cross-linked 125I-uPA-receptor complex all partition in the detergent phase, whereas the unbound ligand remains in the aqueous phase; similar phase partitioning is observed with endogenous uPA-receptor complexes from cultured human and murine cells. Nonidet P-40 26-38 plasminogen activator, urokinase Homo sapiens 155-158 2909535-6 1989 Glycoprotein IIIb was isolated from Triton X-114 platelet membrane extracts, under nondenaturing conditions, by tandem anion-exchange and size exclusion fast protein liquid chromatography. Nonidet P-40 36-48 CD36 molecule Homo sapiens 0-17 3214436-6 1988 Partitioning of endosome fractions between aqueous and Triton X-114 phases indicated that the calmodulin-binding polypeptide was hydrophobic. Nonidet P-40 55-67 calmodulin 1 Rattus norvegicus 94-104 3183059-6 1988 Unlike the macrophage LDL receptor, MBP 190 partitions into the aqueous phase after phase separation of Triton X-114 extracts. Nonidet P-40 104-116 myelin basic protein Homo sapiens 36-39 2843799-3 1988 When the Triton X-114 phase separation technique was employed using membranes from differentiated cells, a small but significant proportion of choline acetyltransferase was recovered in the detergent-rich phase. Nonidet P-40 9-21 choline acetyltransferase Mus musculus 143-168 3049586-7 1988 The soluble alpha-mannosidase arises from a larger hydrophobic form of the enzyme which is found in the detergent phase during partition in Triton X-114. Nonidet P-40 140-152 alpha-mannosidase Saccharomyces cerevisiae S288C 12-29 3171582-2 1988 5-HT1A receptors were solubilized from bovine frontal cortical membranes with 0.3% digitonin and 0.1% Nonidet P-40, and bound effectively to 1-[2-(4-aminophenyl)ethyl]-4-(3-trifluoromethylphenyl)piperazine (PAPP)-coupled Affi-Gel 10 in a time-dependent manner. Nonidet P-40 102-114 5-hydroxytryptamine receptor 1A Bos taurus 0-6 2456917-4 1988 Addition of low concentrations of the detergent Nonidet P-40, used to maintain stability in the purified bovine preparations, however, restored the MIS inhibitory effect to the human preparation, which could, in turn, be blocked by a polyclonal antibody raised to human recombinant MIS; Nonidet P-40 itself caused no inhibition. Nonidet P-40 48-60 anti-Mullerian hormone Bos taurus 148-151 2456917-4 1988 Addition of low concentrations of the detergent Nonidet P-40, used to maintain stability in the purified bovine preparations, however, restored the MIS inhibitory effect to the human preparation, which could, in turn, be blocked by a polyclonal antibody raised to human recombinant MIS; Nonidet P-40 itself caused no inhibition. Nonidet P-40 48-60 anti-Mullerian hormone Homo sapiens 282-285 2456917-4 1988 Addition of low concentrations of the detergent Nonidet P-40, used to maintain stability in the purified bovine preparations, however, restored the MIS inhibitory effect to the human preparation, which could, in turn, be blocked by a polyclonal antibody raised to human recombinant MIS; Nonidet P-40 itself caused no inhibition. Nonidet P-40 287-299 anti-Mullerian hormone Bos taurus 148-151 3355813-5 1988 The 2-oxoglutarate carrier has been purified by hydroxyapatite chromatography after extensive removal of Triton X-114 from the detergent extract. Nonidet P-40 105-117 solute carrier family 25 member 11 Rattus norvegicus 4-26 3388771-1 1988 RNA-dependent RNA polymerase (RdRp) was prepared from brome mosaic virus (BMV)-infected barley by a procedure including Nonidet-P40 treatment. Nonidet P-40 120-131 RNA-dependent RNA polymerase Brome mosaic virus 0-28 3225871-5 1988 Partition experiments with Triton X-114 revealed an amphiphilic behavior for GAP-43 and a strong affinity for hydrophobic environments for pp80 and pp40. Nonidet P-40 27-39 growth associated protein 43 Rattus norvegicus 77-83 2825797-1 1987 Acid sphingomyelinase (sphingomyelin phosphodiesterase, EC 3.1.4.12) was purified from human urine in the presence of 0.1% Nonidet P-40. Nonidet P-40 123-135 sphingomyelin phosphodiesterase 1 Homo sapiens 0-21 2891709-10 1988 All wild-type and class A, C, E, and F mutant cells contain a major Triton X-114 binding species of cell-associated [3H]Thy-1. Nonidet P-40 68-80 Thy-1 cell surface antigen Homo sapiens 120-125 3276514-9 1988 In particular, EF-1 gamma partitions solely into the detergent phase of Triton X-114 solutions. Nonidet P-40 72-84 eukaryotic translation elongation factor 1 gamma Homo sapiens 15-25 2825797-1 1987 Acid sphingomyelinase (sphingomyelin phosphodiesterase, EC 3.1.4.12) was purified from human urine in the presence of 0.1% Nonidet P-40. Nonidet P-40 123-135 sphingomyelin phosphodiesterase 1 Homo sapiens 23-54 3028377-5 1986 Mr and hydrophobicity of the alkaline phosphatase were determined by gel filtration on TSK-250 and partitioning in Triton X-114, respectively. Nonidet P-40 115-127 AT695_RS04080 Staphylococcus aureus 29-49 3693398-9 1987 Since the immediate post-translational precursor, the 65-kD protein, is hydrophilic in nature as shown by its partitioning behavior in a phase-separated Triton X-114 solution, gp 80 is segregated into the apical exocytotic pathway as a soluble molecule. Nonidet P-40 153-165 clusterin Canis lupus familiaris 176-181 3667691-8 1987 The hydrophilic nature of both the complex and free egasyn was confirmed by phase separation experiments with the detergent Triton X-114. Nonidet P-40 124-136 carboxylesterase 1E Mus musculus 52-58 3813562-3 1987 Interaction with Triton X-114 micelles was eliminated or decreased by treatment of intestinal enzyme with phospholipase A2 or papain, while only phosphatidylinositol (PI)-specific phospholipase C (PIPLC) and subtilisin were effective with the kidney enzyme. Nonidet P-40 17-29 phospholipase A2 group IB Rattus norvegicus 106-122 20501065-4 1987 The membrane-bound PCMT could be shown to undergo activation after treatment with Na-deoxycholate and CHAPs, while after its detergent-induced solubilization PCMT activation was observed after Na-deoxycholate, Nonidet P-40 and Lubrol-P(X). Nonidet P-40 210-222 isoprenylcysteine carboxyl methyltransferase Homo sapiens 19-23 20501065-4 1987 The membrane-bound PCMT could be shown to undergo activation after treatment with Na-deoxycholate and CHAPs, while after its detergent-induced solubilization PCMT activation was observed after Na-deoxycholate, Nonidet P-40 and Lubrol-P(X). Nonidet P-40 210-222 isoprenylcysteine carboxyl methyltransferase Homo sapiens 158-162 20501280-3 1988 In guinea pigs receiving septal lesions, a large reduction in both total and in Triton X-114-extractable choline acetyltransferase in hippocampal synaptosomes was observed indicating that the detergent-extracted form is associated with cholinergic nerve terminals. Nonidet P-40 80-92 choline O-acetyltransferase Rattus norvegicus 105-130 20501280-4 1988 When membrane-bound choline acetyltransferase from lysed, washed synaptosomes was incubated in Triton X-114, 30% of the membrane-associated enzyme could be extracted into the detergent-rich phase. Nonidet P-40 95-107 choline O-acetyltransferase Rattus norvegicus 20-45 2446864-2 1987 The soluble form distributes into the Triton X-114-poor aqueous phase, while detergent-solubilized MAG predominantly enters the Triton X-114-rich phase. Nonidet P-40 128-140 myelin-associated glycoprotein Mus musculus 99-102 3912371-0 1985 Isolation of human erythrocyte acetylcholinesterase using phase separation with Triton X-114 and monoclonal immunosorbent chromatography. Nonidet P-40 80-92 acetylcholinesterase (Cartwright blood group) Homo sapiens 31-51 3161894-5 1985 The CLB-HEC 75 antigen was isolated from Nonidet P-40-solubilized endothelial cells and platelets by immunoprecipitation and exhibited an apparent molecular weight by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of approximately 145,000 in the presence of 2-mercaptoethanol. Nonidet P-40 41-53 citramalyl-CoA lyase Homo sapiens 4-7 4019514-1 1985 The mitochondrial dicarboxylate carrier has been substantially purified from rat liver mitoplasts by extraction with Triton X-114 in the presence of cardiolipin followed by chromatography on hydroxylapatite. Nonidet P-40 117-129 solute carrier family 25 member 10 Rattus norvegicus 4-39 3874872-5 1985 An immunobinding assay of Nonidet P-40-solubilized plasma membrane selectively co-isolates gp180 and fodrin. Nonidet P-40 26-38 carboxypeptidase D Mus musculus 91-96 3874872-7 1985 Membrane extraction with Triton X-114 followed by sucrose gradient centrifugation permits isolation of a gp180-fodrin complex with a 1:1 molar ratio and sedimentation coefficient(s) of approximately 20. Nonidet P-40 25-37 carboxypeptidase D Mus musculus 105-110 3782081-0 1986 Demonstration of the amphiphilic character of hormone-sensitive lipase by temperature-induced phase separation in Triton X-114 and charge-shift electrophoresis. Nonidet P-40 114-126 lipase G, endothelial type Rattus norvegicus 64-70 3782081-9 1986 In contrast to ATP-citrate lyase, a reference hydrophilic protein, the lipase was shown to partition predominantly (approximately 80%) into the detergent-rich phase upon phase separation in Triton X-114. Nonidet P-40 190-202 lipase G, endothelial type Rattus norvegicus 71-77 3013678-1 1986 Partial purification of glucose-6-phosphatase from rat liver microsomes by solubilization of the membranes with the non-ionic detergent Triton X-114 at pH 6.5 and the removal of inactivating detergent by hydrophobic chromatography results in a thermostable enzyme protein which is not dependent on stabilizing phospholipids or proteins. Nonidet P-40 136-148 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 24-45 3949882-12 1986 All three mutant proteins have Triton X-114 binding properties similar to the wild-type p62. Nonidet P-40 31-43 nucleoporin 62 Homo sapiens 88-91 3932346-6 1985 We found that pp60v-src was very prone to aggregation; to maintain it as a monomer both Nonidet P-40 and KCl were required. Nonidet P-40 88-100 p60 src Rous sarcoma virus 20-23 2865952-1 1985 Phase separation of synaptic membrane preparations with Triton X-114 reveals the presence of aminopeptidase N. Nonidet P-40 56-68 alanyl aminopeptidase, membrane Sus scrofa 93-109 4029134-6 1985 The integral and peripheral components of guinea-pig MFGM were identified by treating membrane preparations with sodium carbonate solutions at high pH and by partitioning the membrane proteins in solutions of Triton X-114. Nonidet P-40 209-221 milk fat globule EGF and factor V/VIII domain containing Bos taurus 53-57 6317275-2 1984 ACE was solubilized by Nonidet P-40, and assayed by reversible phase high performance liquid chromatography. Nonidet P-40 23-35 angiotensin I converting enzyme Canis lupus familiaris 0-3 3923488-8 1985 Solubilization experiments, as well as phase separation experiments using Triton X-114, indicated that p38 is an integral membrane protein. Nonidet P-40 74-86 mitogen activated protein kinase 14 Rattus norvegicus 103-106 2983763-4 1985 The effect of Triton X-114 on the glucose-6-phosphatase activity was studied systematically and the whole magnitude of time- and temperature-dependent inactivation of this enzyme has been demonstrated. Nonidet P-40 14-26 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 34-55 2983763-7 1985 Kinetic analyses suggest interrelationships between Triton X-114 and the permeability barrier of the glucose-6-phosphatase system. Nonidet P-40 52-64 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 101-122 2983763-8 1985 At 0 degree C 2-propanol and ethanol are more potent tools for membrane modification than Triton X-114 and release 88% and 86% latent activity corresponding to an activation of the glucose-6-phosphatase of about 850% and 700%, respectively. Nonidet P-40 90-102 glucose-6-phosphatase catalytic subunit 1 Rattus norvegicus 181-202 3990704-4 1985 These proteins were partially purified by phase separation in Triton X-114 solution, demonstrating that the p63 of each of the three species is the most abundant integral membrane protein in the promastigote. Nonidet P-40 62-74 transformation related protein 63 Mus musculus 108-111 3155488-1 1985 A murine monoclonal antibody specific for glycoprotein (GP)IIIa was prepared by immunization with a GPIIb- and GPIIIa-enriched Triton X-114 extract of platelet membranes. Nonidet P-40 127-139 integrin subunit beta 3 Homo sapiens 111-117 6238966-0 1984 Phase separation of the receptor for immunoglobulin E and its subunits in Triton X-114. Nonidet P-40 74-86 immunoglobulin heavy constant epsilon Homo sapiens 37-53 6238120-4 1984 The incorporated DAF could not be removed from the red cell surface by repeated washings in the presence of high salt concentration but was solubilized when the stroma were extracted with 0.1% Nonidet P-40. Nonidet P-40 193-205 CD55 molecule (Cromer blood group) Homo sapiens 17-20 6088554-8 1984 Solubilization of affinity-labeled membranes in Nonidet P-40 followed by affinity chromatography on wheat germ agglutinin-agarose revealed that all three CCK-binding proteins specifically interact with this lectin and can be eluted with N-acetyl-D-glucosamine. Nonidet P-40 48-60 cholecystokinin Rattus norvegicus 154-157 6205883-5 1984 Analysis by gel filtration in Nonidet-P40 or deoxycholate revealed the presence of complexes of purified Thy-1 that were specifically precipitated by antibodies against this determinant. Nonidet P-40 30-41 Thy-1 cell surface antigen Homo sapiens 105-110 4041242-0 1985 Insulin receptor protein rendered visible in triton X-114 membranes. Nonidet P-40 45-57 insulin receptor Homo sapiens 0-16 6323736-3 1984 The Nonidet P-40-insoluble fraction was further fractionated in the presence of deoxycholate and Tween 40 to yield a soluble fraction (cytoskeletal) and an insoluble fraction (Nuc), which is primarily cell nuclei. Nonidet P-40 4-16 nucleobindin 1 Homo sapiens 176-179 6140954-8 1984 Further treatment of the membrane fraction with 1% Nonidet P-40 resulted in a partial purification of the ATPase about 15-fold compared to the initial homogenate. Nonidet P-40 51-63 dynein axonemal heavy chain 8 Homo sapiens 106-112 7378064-9 1980 The binding was inhibited when fibronectin was incubated with 40-80% ethylene glycol, the ionic detergents sodium dodecyl sulphate and deoxycholate, and the non-ionic detergents Nonidet P-40, Tween 80 and Triton X-100, all at a concentration of 0.1%. Nonidet P-40 178-190 fibronectin 1 Homo sapiens 31-42 6641908-1 1983 Porcine liver beta-D-glucose dehydrogenase has been isolated using Triton X-114 to release it from the endoplasmic reticulum. Nonidet P-40 67-79 hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Homo sapiens 21-42 6860675-5 1983 The transition temperature, 6.4 degrees C, is close to that of a microsomal membrane phase change, a result that is consistent with the fact that glucose dehydrogenase contains lipid materials when isolated with a non-ionic detergent such as Triton X-114. Nonidet P-40 242-254 hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase Homo sapiens 146-167 6169847-2 1981 Cleavage performed in the presence of 0.15% Nonidet P-40 in water yielded polypeptides of approximately 40,000 (P40) and 25,000 (P25) Mr. Nonidet P-40 44-56 interleukin 9 Homo sapiens 112-115 6169847-2 1981 Cleavage performed in the presence of 0.15% Nonidet P-40 in water yielded polypeptides of approximately 40,000 (P40) and 25,000 (P25) Mr. Nonidet P-40 44-56 tubulin polymerization promoting protein Homo sapiens 129-132 6411352-3 1983 A high yield of 60% recovered activity was achieved in the absence of exogenous carrier protein by stabilizing MIS with 2-mercaptoethanol, EDTA, and Nonidet-P40 and eliminating losses in the handling and concentration of MIS fractions. Nonidet P-40 149-160 anti-Mullerian hormone Homo sapiens 111-114 6183264-2 1982 Chymotrypsin, trypsin, or papain treatment in buffer containing Nonidet P-40 resulted in removal of discrete segments from the H-2 heavy chain without detectable alteration of the beta 2-microglobulin. Nonidet P-40 64-76 relaxin 2 Homo sapiens 127-130 6262300-5 1981 A proteolytic fragment (Mr = 70,000) derived from the portion of the transferrin receptor exposed on the cell surface can be obtained by trypsin digestion of intact or Nonidet P-40-solubilized cells. Nonidet P-40 168-180 transferrin Homo sapiens 69-80 7370241-8 1980 About 14--15 proteins (including fibronectin) were released by Nonidet P40/urea/ampholyte/mercaptoethanol but not by Nonidet P40. Nonidet P-40 63-74 fibronectin 1 Homo sapiens 33-44 115689-3 1979 Triton X-100 and Nonidet P-40 extracted up to 60% of the protein including the major membrane glycoproteins and the enzymes trehalase, maltase and aminopeptidase (microsomal). Nonidet P-40 17-29 trehalase Oryctolagus cuniculus 124-133 29306844-7 2018 The specific reverse solute flux (Js/Jw) of 40% PPG-725 doped with Triton X-114 was found to be 0.01 g/L, considerably much lesser than the conventional inorganic draw agents. Nonidet P-40 67-79 serglycin Homo sapiens 48-51 30890336-0 2019 Non-ionic detergents Nonidet P-40 and Triton X-100 increase enzymatic activity of plasmin. Nonidet P-40 21-33 plasminogen Homo sapiens 82-89 30426406-2 2019 Here we demonstrate the differences in extraction of the focal adhesion protein Kindlin-2 and the transcriptional repressor Snail from choriocarcinoma cells using NP-40 and RIPA lysis buffer. Nonidet P-40 163-168 FERM domain containing kindlin 2 Homo sapiens 80-89 1259536-6 1976 For NP-40-solubilized H-2d antigen, about 34% of helix, 13% beta sheet and 41% turns was found. Nonidet P-40 4-9 histocompatibility 2, D region Mus musculus 22-26 4833609-5 1974 The 110S structure with which the DNA reaction product remains associated in Nonidet P-40-treated preparations was identified as Dane particle core by immunoprecipitation with serum containing anti-HB(c). Nonidet P-40 77-89 keratin 88, pseudogene Homo sapiens 198-203 31350430-5 2019 We first demonstrate that SynGAP-containing interactions are more abundant in 1% Deoxycholate (DOC), while Shank-, Homer- and mGluR5-containing interactions are more abundant in 1% NP-40 or Triton. Nonidet P-40 181-186 SH3 and multiple ankyrin repeat domains 2 Homo sapiens 107-112 31077118-5 2018 The prepared Fab was purified in several steps, desalted, and lipopolysaccharide-depleted using ammonium sulfate solution and dialysis against phosphate buffer and Triton X-114 solution, respectively. Nonidet P-40 164-176 FA complementation group B Homo sapiens 13-16 28934999-2 2018 Ammonium pyrrolidinedithiocarbamate was used as a ligand to make a hydrophobic complex of Cd+2, which was extracted in an ionic liquid (1-butyl-3-methylimidazolium hexafluorophosphate), and the nonionic surfactant Triton X-114 was applied as a dispersing medium. Nonidet P-40 214-226 CD2 molecule Homo sapiens 90-94 29306844-8 2018 Finally, membrane distillation operation was utilized as the recovery system in which solute rejection of 97% was achieved for 40% PPG-725/Triton X-114. Nonidet P-40 139-151 serglycin Homo sapiens 131-134 29306844-9 2018 Therefore, the overall performance supported PPG-725/Triton X-114 as being an efficient draw agent for forward osmosis-membrane distillation hybrid process. Nonidet P-40 53-65 serglycin Homo sapiens 45-48 28355240-4 2017 However, remaining TX-114 was found to interfere with LPS and protein concentration assays and decreased viability of THP-1 macrophages and HEK-Blue 293 cells. Nonidet P-40 19-25 GLI family zinc finger 2 Homo sapiens 118-123 24225581-4 2013 The results of zeta potential and (19)F NMR measurements indicated that the anion NTf2(-) penetrated into the TX-114 micelles and was enriched in the surfactant-rich phase during the CPE process. Nonidet P-40 110-116 nuclear transport factor 2 Homo sapiens 82-86 26043989-2 2015 Here we demonstrate the differences in extraction of the focal adhesion protein Kindlin-2 from choriocarcinoma cells using NP-40 and RIPA lysis buffer. Nonidet P-40 123-128 FERM domain containing kindlin 2 Homo sapiens 80-89 24548619-8 2014 Moreover, MMP-27 was protected from digestion by proteinase K. Finally, MT1-MMP/MMP-14 but neither endogenous nor recombinant MMP-27 partitioned in the detergent phase after Triton X-114 extraction, indicating that MMP-27 is not an integral membrane protein. Nonidet P-40 174-186 matrix metallopeptidase 27 Homo sapiens 10-16 24548619-8 2014 Moreover, MMP-27 was protected from digestion by proteinase K. Finally, MT1-MMP/MMP-14 but neither endogenous nor recombinant MMP-27 partitioned in the detergent phase after Triton X-114 extraction, indicating that MMP-27 is not an integral membrane protein. Nonidet P-40 174-186 matrix metallopeptidase 14 Homo sapiens 72-79 26224100-6 2015 METHODS: Co-localisation of Rac1 and VAV2 was determined by Triton X-114 phase partition and confocal microscopy. Nonidet P-40 60-72 Rac family small GTPase 1 Rattus norvegicus 28-32 26224100-6 2015 METHODS: Co-localisation of Rac1 and VAV2 was determined by Triton X-114 phase partition and confocal microscopy. Nonidet P-40 60-72 vav guanine nucleotide exchange factor 2 Rattus norvegicus 37-41 26242641-6 2015 We found that a lysis solution containing both the non-ionic detergent (IGEPAL CA-630, chemically equivalent to Nonidet P-40 or NP-40) and bovine serum albumin (BSA) gave the best RT-qPCR yield. Nonidet P-40 128-133 albumin Homo sapiens 146-159 25857898-2 2015 The cloud point extraction (CPE) method is based on the formation of PdI2 which is then entrapped in the non-ionic surfactant Triton X-114. Nonidet P-40 126-138 peptidyl arginine deiminase 2 Homo sapiens 69-73 24992923-2 2014 The d-CPE procedure was based on forming complexes of elemental ions with complexing reagent 1-(2-pyridylazo)-2-naphthol (PAN), and subsequent entrapping the complexes in nonionic surfactant (Triton X-114). Nonidet P-40 192-204 carboxypeptidase E Homo sapiens 6-9 24574259-1 2014 The effect of Triton X-114 on the physicochemical properties of a single-chain antibody fragment (scFv) has been studied. Nonidet P-40 14-26 immunglobulin heavy chain variable region Homo sapiens 98-102 22875972-5 2012 A11 partitioned almost equally between the detergent and aqueous phases upon Triton X-114 phase separation, demonstrating an intrinsic affinity with lipids. Nonidet P-40 77-89 DXS435E Homo sapiens 0-3 23772752-6 2013 MICA*008 processing is also unusual as it is observed in the endoplasmic reticulum as a Triton X-114 soluble protein, partially undergoing GPI modification while the rest is exocytosed, suggesting a new model for MICA*008 release. Nonidet P-40 88-101 MHC class I polypeptide-related sequence A Homo sapiens 0-4 23772752-6 2013 MICA*008 processing is also unusual as it is observed in the endoplasmic reticulum as a Triton X-114 soluble protein, partially undergoing GPI modification while the rest is exocytosed, suggesting a new model for MICA*008 release. Nonidet P-40 88-101 MHC class I polypeptide-related sequence A Homo sapiens 214-218 22847478-4 2012 In this study, a novel approach was used in the context of an aqueous two-phase micellar system comprised of the nonionic surfactant Triton X-114 to concentrate a model protein, namely transferrin, prior to LFA. Nonidet P-40 133-145 transferrin Homo sapiens 185-196 22860206-3 2012 In the in vitro assays thus far performed, the maximal activity of NAAA was achieved in the presence of both nonionic detergent (Triton X-100 or Nonidet P-40) and the SH reagent dithiothreitol. Nonidet P-40 145-157 N-acylethanolamine acid amidase Mus musculus 67-71