PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 24009622-5 2013 We found that 5% ethanol did not reduce ABCG2 protein levels, but significantly reduced ABCG2 protein function by a Hoechst 33342 extrusion assay, an ATPase activity assay, and transmission electron microscopy. bisbenzimide ethoxide trihydrochloride 116-129 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 88-93 23636893-4 2013 Results from Hoechst 33342 staining and flow cytometry assay showed that AMA induced apoptosis significantly in PC12 cells, while pretreatment with LIF (0.5 and 1 ng/ml) can attenuate this injury. bisbenzimide ethoxide trihydrochloride 13-26 LIF, interleukin 6 family cytokine Rattus norvegicus 148-151 24900683-4 2013 Inhibition of ABCB1 and ABCG2 was determined in a calcein-AM and a Hoechst 33342 microplate assay, respectively. bisbenzimide ethoxide trihydrochloride 67-80 ATP binding cassette subfamily B member 1 Homo sapiens 14-19 24900683-4 2013 Inhibition of ABCB1 and ABCG2 was determined in a calcein-AM and a Hoechst 33342 microplate assay, respectively. bisbenzimide ethoxide trihydrochloride 67-80 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 24-29 23484311-6 2013 Hoechst 33342 fluorescence staining, flow cytometry assay test and Western-blot proved HMGN2 could make Tca8113 cells morphological change, make Tca8113 cells block in S period of cell cycle and strongly promote Tca8113 cells to apoptosis. bisbenzimide ethoxide trihydrochloride 0-13 high mobility group nucleosomal binding domain 2 Homo sapiens 87-92 22930410-6 2013 In the FACS analysis of cells stained with Pyronin Y and Hoechst 33342, GLUL-overexpressing cells showed lower population of cells in the G0-quiescent phase than mock cells (5-12%). bisbenzimide ethoxide trihydrochloride 57-70 glutamate-ammonia ligase Homo sapiens 72-76 23268645-8 2013 The kinetics of LDS-751 and Hoechst 33342 transport by reconstituted Pgp was monitored using a real-time fluorescence-based assay to obtain apparent turnover frequencies. bisbenzimide ethoxide trihydrochloride 28-41 phosphoglycolate phosphatase Homo sapiens 69-72 23271277-2 2012 Hoechst 33342 and PI were used to detect the morphological changes in the cell death induced by the two types of TNF-alpha. bisbenzimide ethoxide trihydrochloride 0-13 tumor necrosis factor Homo sapiens 113-122 23150210-6 2013 The inhibitory activities against BCRP and P-gp were assayed using a Hoechst 33342 assay for BCRP and a calcein AM assay for P-gp. bisbenzimide ethoxide trihydrochloride 69-82 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 34-38 23150210-6 2013 The inhibitory activities against BCRP and P-gp were assayed using a Hoechst 33342 assay for BCRP and a calcein AM assay for P-gp. bisbenzimide ethoxide trihydrochloride 69-82 phosphoglycolate phosphatase Homo sapiens 43-47 23807847-4 2013 Moreover, Hoechst 33342 staining implicated that CTD-NSVs induced higher apoptotic rates in MCF-7 cells than free CTD solution. bisbenzimide ethoxide trihydrochloride 10-23 CTD Homo sapiens 49-52 22942241-6 2012 Intracellular retention of fluorescent rhodamine 123, Hoechst 33342, and calcein acetoxymethyl ester was increased in LPS-treated microglia, suggesting that the functions of Mdr1, Bcrp, and Mrps were decreased, respectively. bisbenzimide ethoxide trihydrochloride 54-67 toll-like receptor 4 Mus musculus 118-121 23460964-4 2012 Invert fluorescent microscope was used to observe the efflux of fluorescence dye Hoechst 33342 by BCRP, furthermore, the BCRP"s inhibitor GF120918 was applied to reverse the efflux of Hoechst 33342. bisbenzimide ethoxide trihydrochloride 81-94 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 98-102 23205118-8 2012 Hoechst 33342 staining and flow cytometry revealed that the effects on L-OHP-induced apoptosis were enhanced by the overexpression of BNIP3. bisbenzimide ethoxide trihydrochloride 0-13 BCL2 interacting protein 3 Homo sapiens 134-139 23014881-10 2012 The efflux of Hoechst 33342, a substrate for MDR1, was blocked by MDR1 inhibitor cyclosporin A, suggesting the functional expression of this transporter. bisbenzimide ethoxide trihydrochloride 14-27 ATP binding cassette subfamily B member 1 Homo sapiens 45-49 23014881-10 2012 The efflux of Hoechst 33342, a substrate for MDR1, was blocked by MDR1 inhibitor cyclosporin A, suggesting the functional expression of this transporter. bisbenzimide ethoxide trihydrochloride 14-27 ATP binding cassette subfamily B member 1 Homo sapiens 66-70 23460964-4 2012 Invert fluorescent microscope was used to observe the efflux of fluorescence dye Hoechst 33342 by BCRP, furthermore, the BCRP"s inhibitor GF120918 was applied to reverse the efflux of Hoechst 33342. bisbenzimide ethoxide trihydrochloride 81-94 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 121-125 23460964-4 2012 Invert fluorescent microscope was used to observe the efflux of fluorescence dye Hoechst 33342 by BCRP, furthermore, the BCRP"s inhibitor GF120918 was applied to reverse the efflux of Hoechst 33342. bisbenzimide ethoxide trihydrochloride 184-197 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 98-102 23460964-4 2012 Invert fluorescent microscope was used to observe the efflux of fluorescence dye Hoechst 33342 by BCRP, furthermore, the BCRP"s inhibitor GF120918 was applied to reverse the efflux of Hoechst 33342. bisbenzimide ethoxide trihydrochloride 184-197 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 121-125 22644857-3 2012 GR (10-50 mug/mL) prevented the Abeta (25-35)-induced neuronal apoptotic death, as assessed by a MTT assay and Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 111-124 amyloid beta precursor protein Rattus norvegicus 32-37 22441796-6 2012 Furthermore, although epididymal sperm ABCG2 was shown to be functional, as determined by its ability to extrude Hoechst 33342 in the presence of the specific inhibitor Fumitremorgin C, ABCG2 present in ejaculated sperm was found to be nonfunctional. bisbenzimide ethoxide trihydrochloride 113-126 ATP binding cassette subfamily G member 2 Bos taurus 39-44 22407964-2 2012 Side population (SP) sorting using an ultraviolet (UV) laser is an established method to isolate CSC based on ABC drug transporter-dependent (e.g., ABCG2) Hoechst 33342 efflux. bisbenzimide ethoxide trihydrochloride 155-168 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 148-153 23153066-3 2012 METHODS: In cell lines derived from human hepatocellular carcinoma, ABCG2 gene expression was assessed by reverse transcription quantitative real time PCR and function by Hoechst 33342 efflux assay; protein content was assessed by SDS-PAGE Western blot. bisbenzimide ethoxide trihydrochloride 171-184 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 68-73 23311140-3 2012 The anti-apoptotic potential of Ad-Nkx2.5 was validated by MTT assay for cell viability, Hoechst33342 staining for cellular morphology, and immunoblotting for caspase-3 activity. bisbenzimide ethoxide trihydrochloride 89-101 NK2 homeobox 5 Rattus norvegicus 35-41 22561258-4 2012 Western blotting and immunofluorescence results indicated that the expression of PI3-K and Akt was promoted in the DMH group while least apoptosis was detected in this group as analyzed by Hoechst 33342-propidium iodide co-staining. bisbenzimide ethoxide trihydrochloride 189-202 AKT serine/threonine kinase 1 Rattus norvegicus 91-94 21139581-6 2011 Quantitative non-invasive magnetic resonance imaging and fluorescence microscopy of tumour uptake of Hoechst 33342, and haematoxylin and eosin staining, revealed significantly impaired vascular development and function in antisense iNOS tumours compared with control in vivo, primarily associated with the more necrotic tumour core. bisbenzimide ethoxide trihydrochloride 101-114 nitric oxide synthase 2 Homo sapiens 232-236 22674135-2 2012 Side population (SP) cells, which pump out the fluorescent dye Hoechst 33342 (H33342) via the ABCG2 transporter, define a fraction of adult tissue stem cells in a wide variety of organs. bisbenzimide ethoxide trihydrochloride 63-76 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 94-99 22674135-2 2012 Side population (SP) cells, which pump out the fluorescent dye Hoechst 33342 (H33342) via the ABCG2 transporter, define a fraction of adult tissue stem cells in a wide variety of organs. bisbenzimide ethoxide trihydrochloride 78-84 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 94-99 21596996-7 2011 Additionally, Slfn-3 transfection of FOLFOX-resistant HCT-116 and HT-29 cells reduced Hoechst 33342 dye exclusion. bisbenzimide ethoxide trihydrochloride 86-99 schlafen family member 12 Homo sapiens 14-20 21566063-6 2011 YHO-13177 increased the intracellular accumulation of Hoechst 33342, a substrate of BCRP, at 30 minutes and partially suppressed the expression of BCRP protein at more than 24 hours after its treatment in both HCT116/BCRP and A549/SN4 cells. bisbenzimide ethoxide trihydrochloride 54-67 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 84-88 21570282-4 2011 Among these esters highly potent and selective ABCG2 modulators were identified (inhibition of ABCB1 and ABCG2 determined in the calcein-AM and the Hoechst 33342 microplate assay, respectively). bisbenzimide ethoxide trihydrochloride 148-161 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 47-52 21570282-4 2011 Among these esters highly potent and selective ABCG2 modulators were identified (inhibition of ABCB1 and ABCG2 determined in the calcein-AM and the Hoechst 33342 microplate assay, respectively). bisbenzimide ethoxide trihydrochloride 148-161 ATP binding cassette subfamily B member 1 Homo sapiens 95-100 21570282-4 2011 Among these esters highly potent and selective ABCG2 modulators were identified (inhibition of ABCB1 and ABCG2 determined in the calcein-AM and the Hoechst 33342 microplate assay, respectively). bisbenzimide ethoxide trihydrochloride 148-161 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 105-110 21384159-0 2011 Hoechst 33342 induced reactive oxygen species and impaired expression of cytochrome c oxidase subunit 1 leading to cell death in irradiated human cancer cells. bisbenzimide ethoxide trihydrochloride 0-13 cytochrome c, somatic Homo sapiens 73-85 21384159-4 2011 Mn-SOD and Catalase, and release of cytochrome c into the cytosol, were observed in time-dependent manner when cells were irradiated (5 Gy) in presence of Hoechst 33342. bisbenzimide ethoxide trihydrochloride 155-168 superoxide dismutase 2 Homo sapiens 0-19 21384159-4 2011 Mn-SOD and Catalase, and release of cytochrome c into the cytosol, were observed in time-dependent manner when cells were irradiated (5 Gy) in presence of Hoechst 33342. bisbenzimide ethoxide trihydrochloride 155-168 cytochrome c, somatic Homo sapiens 36-48 21384159-6 2011 Treatment with antioxidants PEG-MnSOD and PEG-catalase inhibited the increase in ROS and loss of cell survival, suggesting the involvement of ROS in the Hoechst 33342-induced cell death. bisbenzimide ethoxide trihydrochloride 153-166 superoxide dismutase 2 Homo sapiens 32-37 21436125-4 2011 In rat PT or Madin-Darby canine kidney (MDCK) cells overexpressing ABCB1, ABCB1-dependent efflux of rhodamine 123(+) (Rh123(+)) or (109)Cd(2+) were determined, and cell death was assayed with MTT, H-33342 nuclear staining, and monolayer integrity by impedance sensing (Electric cell-substrate impedance sensing [ECIS]). bisbenzimide ethoxide trihydrochloride 197-204 ATP binding cassette subfamily B member 1 Canis lupus familiaris 67-72 21436125-4 2011 In rat PT or Madin-Darby canine kidney (MDCK) cells overexpressing ABCB1, ABCB1-dependent efflux of rhodamine 123(+) (Rh123(+)) or (109)Cd(2+) were determined, and cell death was assayed with MTT, H-33342 nuclear staining, and monolayer integrity by impedance sensing (Electric cell-substrate impedance sensing [ECIS]). bisbenzimide ethoxide trihydrochloride 197-204 ATP binding cassette subfamily B member 1 Canis lupus familiaris 74-79 24212798-4 2011 SP cells are identified using dual wavelength flow cytometry combined with Hoechst 33342 dye efflux, this ability is due to expression of one or more members of the ABC transporter family. bisbenzimide ethoxide trihydrochloride 75-88 ATP binding cassette subfamily B member 6 (Langereis blood group) Homo sapiens 165-168 22248732-4 2012 BCRP expression appears to be a characteristic of certain normal tissue stem cells termed "side population cells," which are identified on flow cytometric analysis by their ability to exclude Hoechst 33342, a BCRP substrate fluorescent dye. bisbenzimide ethoxide trihydrochloride 192-205 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 0-4 22200670-3 2012 The accelerated transport activity of Hoechst33342, an ABCG2/BCRP substrate, in HeLa/SN100 cells was significantly decreased by the presence of the calcium antagonists, except for diltiazem, nifedipine or verapamil, returning to the level of HeLa cells. bisbenzimide ethoxide trihydrochloride 38-50 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 67-72 22200670-3 2012 The accelerated transport activity of Hoechst33342, an ABCG2/BCRP substrate, in HeLa/SN100 cells was significantly decreased by the presence of the calcium antagonists, except for diltiazem, nifedipine or verapamil, returning to the level of HeLa cells. bisbenzimide ethoxide trihydrochloride 38-50 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 73-77 22173067-4 2012 Hoechst 33342 and glyburide were used as model ABCG2 substrates for these experiments. bisbenzimide ethoxide trihydrochloride 0-13 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 47-52 21683092-9 2011 Neuroprotective action of MAPK/ERK1/2 and calpain inhibitors were connected with attenuation of LC-induced DNA fragmentation measured by Hoechst 33342 staining and TUNEL assay. bisbenzimide ethoxide trihydrochloride 137-150 mitogen-activated protein kinase 3 Homo sapiens 31-37 21774076-4 2011 In addition, cyclopamine is able to modulate, along with oxysterols and other products, the ABCG2 transporter by increasing Ho342 and mitoxantrone uptake. bisbenzimide ethoxide trihydrochloride 124-129 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 92-97 21774076-5 2011 Therefore, if the SP is solely measured as a Ho342 dye extruding fraction, this may be significantly modulated by the inhibition of ABCG2 transport fraction, independently from the action of cyclopamine on the Hh pathway. bisbenzimide ethoxide trihydrochloride 45-50 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 132-137 22177045-2 2011 METHODS: CD133(+)SP and CD133(-)SP subpopulation was detected and isolated from Hep-2 cell line using Hoechst33342 dye and phycoerythrin (PE)-conjugated CD133 monoclonal antibody assisted by fluorescence activated cell sorting technology. bisbenzimide ethoxide trihydrochloride 102-114 prominin 1 Homo sapiens 9-14 22152314-5 2011 MiR-221 inhibitors significantly inhibited the cell growth and miR-221 mimics increased cell viability 48 hours post-transfection measured by both CellTiter-blue cell viability kit and Hoechst 33342/PI assay (P is less than to 0.05). bisbenzimide ethoxide trihydrochloride 185-198 microRNA 221 Homo sapiens 0-7 22152314-5 2011 MiR-221 inhibitors significantly inhibited the cell growth and miR-221 mimics increased cell viability 48 hours post-transfection measured by both CellTiter-blue cell viability kit and Hoechst 33342/PI assay (P is less than to 0.05). bisbenzimide ethoxide trihydrochloride 185-198 microRNA 221 Homo sapiens 63-70 22152314-8 2011 More cell apoptosis and necrosis were significantly induced by miR-221 inhibitors 48 hours post-transfection detected by both Hoechst 33342/PI assay and flow cytometry PE Annexin V kit (P is less than to 0.05). bisbenzimide ethoxide trihydrochloride 126-139 microRNA 221 Homo sapiens 63-70 21459080-6 2011 Upon further investigation, it was confirmed that nutlin-3a increased the intracellular accumulation of BCRP substrates such as mitoxantrone and Hoechst 33342 in cells expressing functional BCRP without altering the expression level or localization of BCRP. bisbenzimide ethoxide trihydrochloride 145-158 ATP binding cassette subfamily G member 2 Canis lupus familiaris 104-108 21192924-5 2011 Hoechst 33342 assay and flow cytometry further showed that rTFPI-2 induced apoptosis in cultured macrophages in a dose-dependent manner. bisbenzimide ethoxide trihydrochloride 0-13 tissue factor pathway inhibitor 2 Rattus norvegicus 59-66 21287580-6 2011 Using MTT assay, Western blot, Hoechst 33342 staining assay and flow cytometry, we found that silencing of UCH37 in A549 cells induced apoptosis. bisbenzimide ethoxide trihydrochloride 31-44 ubiquitin C-terminal hydrolase L5 Homo sapiens 107-112 20538997-4 2010 Flow cytometry was used to determine the presence of side population (SP) cells based on the ability of ABCG2 to efflux Hoechst 33342 dye. bisbenzimide ethoxide trihydrochloride 120-133 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 104-109 21998702-7 2011 H342-induced apoptosis occurs in a dose-dependent fashion and is associated with morphological changes, caspase-3 activation, cytochrome c mitochondrial translocation, and cleavage of apoptosis-associated proteins. bisbenzimide ethoxide trihydrochloride 0-4 caspase 3 Homo sapiens 104-113 21998702-7 2011 H342-induced apoptosis occurs in a dose-dependent fashion and is associated with morphological changes, caspase-3 activation, cytochrome c mitochondrial translocation, and cleavage of apoptosis-associated proteins. bisbenzimide ethoxide trihydrochloride 0-4 cytochrome c, somatic Homo sapiens 126-138 20623213-8 2010 The effects of the Cys431Leu variation, due to MDR1 (GT1292-3TG) nucleotide transition, on P-gp-dependent intracellular substrate accumulation appeared to be substrate dependent where doxorubicin, vinblastine, and paclitaxel exhibit an increased accumulation (p < 0.05), while verapamil and Hoechst33342 exhibit a decreased intracellular concentration compared with wild type (p < 0.05). bisbenzimide ethoxide trihydrochloride 294-306 ATP binding cassette subfamily B member 1 Homo sapiens 47-51 20623213-8 2010 The effects of the Cys431Leu variation, due to MDR1 (GT1292-3TG) nucleotide transition, on P-gp-dependent intracellular substrate accumulation appeared to be substrate dependent where doxorubicin, vinblastine, and paclitaxel exhibit an increased accumulation (p < 0.05), while verapamil and Hoechst33342 exhibit a decreased intracellular concentration compared with wild type (p < 0.05). bisbenzimide ethoxide trihydrochloride 294-306 ATP binding cassette subfamily B member 1 Homo sapiens 91-95 20458753-13 2010 Finally, flow cytometry and Hoechst 33342 DNA staining demonstrated decreased apoptosis and caspase-3 expression levels in PC-3(GDF-9exp.) bisbenzimide ethoxide trihydrochloride 28-41 growth differentiation factor 9 Homo sapiens 128-133 20879984-2 2010 Moreover, a small number of cancer cells express stem cell markers, including CD133 and ATP-binding cassette transporters through which the cells can pump out anti-cancer drugs or specific fluorescence dyes such as Hoechst33342, suggesting that either cancer cells resemble stem cells or that cancers contain stem cell-like cancer cells, called cancer-initiating cells (CICs) or cancer stem cells. bisbenzimide ethoxide trihydrochloride 215-227 prominin 1 Mus musculus 78-83 20975373-4 2010 The growth-inhibitory effect of the topoisomerase I inhibitor Hoechst 33342, which is extruded by ABCG2, was also investigated in these cells using 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay. bisbenzimide ethoxide trihydrochloride 62-75 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 98-103 20573570-6 2010 [(14)C] Erythromycin was selected as a model substrate for P-gp and MRP2 whereas Hoechst 33342 was employed as a substrate for BCRP. bisbenzimide ethoxide trihydrochloride 81-94 ATP binding cassette subfamily G member 2 Canis lupus familiaris 127-131 19670287-1 2009 The multidrug transporter ABCG2 in cell membranes enables various stem cells and cancer cells to efflux chemicals, including the fluorescent dye Hoechst 33342. bisbenzimide ethoxide trihydrochloride 145-158 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 26-31 20203106-6 2010 After normalization to BCRP levels, the activities of K452A and H457A in effluxing mitoxantrone, boron-dipyrromethene-prazosin, and Hoechst33342 were increased approximately 2- to 6-fold compared with those of wild-type BCRP, whereas the activities of K453D and R465A were decreased by 40 to 60%. bisbenzimide ethoxide trihydrochloride 132-144 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 23-27 20203106-6 2010 After normalization to BCRP levels, the activities of K452A and H457A in effluxing mitoxantrone, boron-dipyrromethene-prazosin, and Hoechst33342 were increased approximately 2- to 6-fold compared with those of wild-type BCRP, whereas the activities of K453D and R465A were decreased by 40 to 60%. bisbenzimide ethoxide trihydrochloride 132-144 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 220-224 20040597-6 2010 In SP cells, which expel H33342, down-regulation of SOX17 was less pronounced than in NSP cells, which retain H33342. bisbenzimide ethoxide trihydrochloride 25-31 SRY-box transcription factor 17 Homo sapiens 52-57 19782742-12 2010 Uptake of [3H]-mitoxantrone was elevated significantly in the presence of GF120918 and fumitremorgin C. An increase in the accumulation of Hoechst 33342, a fluorescent dye was also detected in the presence of BCRP inhibitors when compared to control. bisbenzimide ethoxide trihydrochloride 139-152 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 209-213 20030709-7 2010 PHB was expressed in beta-cells under normal conditions and colocalized with Hoechst 33342 in the nucleus and with the mitochondrial probe Mitofluor in the perinuclear area. bisbenzimide ethoxide trihydrochloride 77-90 prohibitin 1 Rattus norvegicus 0-3 20385068-2 2010 SP cells are characterised by high efflux capability for Hoechst 33342 dye and for anti-cancer therapeutic agents through transporters; ABCG2 (ATP-binding cassette transporter G2) is currently most closely associated with the SP phenotype. bisbenzimide ethoxide trihydrochloride 57-70 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 143-178 19802874-0 2009 ABCG2-associated resistance to Hoechst 33342 and topotecan in a murine cell model with constitutive expression of side population characteristics. bisbenzimide ethoxide trihydrochloride 31-44 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 0-5 19802874-1 2009 Drug resistant tumor "side-populations," enriched in cancer stem cells and identified by reduced accumulation of Hoechst 33342 under ABCG2-mediated efflux, may compromise therapeutic outcome. bisbenzimide ethoxide trihydrochloride 113-126 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 133-138 19802874-7 2009 Hoechst 33342-resistant murine cells showed lower but significant crossresistance to topotecan, again attributable to enhanced ABCG2 expression, enabling cells to evade S-phase arrest. bisbenzimide ethoxide trihydrochloride 0-13 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 127-132 19802874-8 2009 Hoechst 33342/TPT-resistant cells showed limited ancillary gene expression changes that could modify cellular capacity to cope with chronic stress including over-expression of Aldh1a1 and Mgst1, but under-expression of Plk2 and Nnt. bisbenzimide ethoxide trihydrochloride 0-13 aldehyde dehydrogenase family 1, subfamily A1 Mus musculus 176-183 19802874-8 2009 Hoechst 33342/TPT-resistant cells showed limited ancillary gene expression changes that could modify cellular capacity to cope with chronic stress including over-expression of Aldh1a1 and Mgst1, but under-expression of Plk2 and Nnt. bisbenzimide ethoxide trihydrochloride 0-13 microsomal glutathione S-transferase 1 Mus musculus 188-193 19802874-8 2009 Hoechst 33342/TPT-resistant cells showed limited ancillary gene expression changes that could modify cellular capacity to cope with chronic stress including over-expression of Aldh1a1 and Mgst1, but under-expression of Plk2 and Nnt. bisbenzimide ethoxide trihydrochloride 0-13 polo like kinase 2 Mus musculus 219-223 20118824-4 2010 Activity of ABCG2 was measured by Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 34-47 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 12-17 19520157-8 2009 The ABCG2 substrate Hoechst 33342 inhibited extracellular GSH increase after 15dPGJ(2) treatment. bisbenzimide ethoxide trihydrochloride 20-33 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 4-9 19508117-5 2009 Examination of Hoechst 33342-stained samples demonstrated that PB1 on the surfaces of zona-free bovine oocytes were always located near the maternal chromosomes. bisbenzimide ethoxide trihydrochloride 15-28 polybromo 1 Bos taurus 63-66 19268480-3 2009 EPO (2U/ml) in combination with Abeta(25-35) increased the cell viability and reduced the number of apoptotic cells by MTT assay, Trypan blue dye exclusion method, TUNEL staining and Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 183-196 erythropoietin Rattus norvegicus 0-3 24855533-5 2009 Thus, we compared the Hoechst 33342-stained side population (SP) cells in murine adipose-tissue (AT-SP cells) to the SP cells from murine bone marrow (BM-SP cells). bisbenzimide ethoxide trihydrochloride 22-35 WD and tetratricopeptide repeats 1 Mus musculus 81-88 19270533-1 2009 The efflux of Hoechst 33342 by ATP-binding cassette protein G2 (ABCG2) membrane pump allows reproducible identification of a subpopulation of cells by flow cytometric analysis termed the "side population" (SP). bisbenzimide ethoxide trihydrochloride 14-27 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 31-62 19270533-1 2009 The efflux of Hoechst 33342 by ATP-binding cassette protein G2 (ABCG2) membrane pump allows reproducible identification of a subpopulation of cells by flow cytometric analysis termed the "side population" (SP). bisbenzimide ethoxide trihydrochloride 14-27 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 64-69 19287999-4 2009 Both CD133+ and CD133- cells of A549 and H446 possessed stem cell characteristics and exposure to Hoechst 33342 affected the clonogenicity and proliferation of single A549 and H446 cell. bisbenzimide ethoxide trihydrochloride 98-111 prominin 1 Homo sapiens 16-21 19032939-1 2009 An increasingly exploited strategy for the isolation of stem cells is based on the increased efflux of Hoechst 33342 lipophilic dye mediated by ABCG2, an ATP-binding cassette transporter which is highly expressed in various stem cells. bisbenzimide ethoxide trihydrochloride 103-116 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 144-149 19239227-5 2009 In parallel to the alteration in gene expression, functional activity studies indicated that the cellular uptake of benzoic acid, MPP+, calcein-AM, H33342, representative substrates of MCT1, OCT1, MRPs and BCRP, respectively, was significantly (p < 0.05) altered by c-Myc overexpression. bisbenzimide ethoxide trihydrochloride 148-154 solute carrier family 16 member 1 Homo sapiens 185-189 19239227-5 2009 In parallel to the alteration in gene expression, functional activity studies indicated that the cellular uptake of benzoic acid, MPP+, calcein-AM, H33342, representative substrates of MCT1, OCT1, MRPs and BCRP, respectively, was significantly (p < 0.05) altered by c-Myc overexpression. bisbenzimide ethoxide trihydrochloride 148-154 MYC proto-oncogene, bHLH transcription factor Homo sapiens 269-274 19032939-5 2009 Experiments using the Hoechst 33342 ABCG2 substrate and the ABCG2-specific inhibitor FTC demonstrated efflux activity of ABCG2 in mature sperm. bisbenzimide ethoxide trihydrochloride 22-35 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 36-41 18759062-6 2009 The Apoptotic cells were assessed by Hoechst 33342 staining with 25 and 40 microM concentrations of DADS for 24 h. The results have shown that DADS at 25 and 40 microM concentrations has induced the activation of caspases. bisbenzimide ethoxide trihydrochloride 37-50 caspase 9 Homo sapiens 213-221 19582433-4 2009 Constitutive and induced MDR activity can be detected in cellular subsets of disaggregated tissues, using the fluorescent substrates Rhodamine 123 and Hoechst 33342 for ABCB1 (also known as P-gp and MDR1) and ABCG2 (BCRP1). bisbenzimide ethoxide trihydrochloride 151-164 ATP binding cassette subfamily B member 1 Homo sapiens 169-174 19582433-4 2009 Constitutive and induced MDR activity can be detected in cellular subsets of disaggregated tissues, using the fluorescent substrates Rhodamine 123 and Hoechst 33342 for ABCB1 (also known as P-gp and MDR1) and ABCG2 (BCRP1). bisbenzimide ethoxide trihydrochloride 151-164 phosphoglycolate phosphatase Homo sapiens 190-194 18930752-9 2008 Functional expression of MDR1 and BCRP at the apical membrane was also demonstrated using a Hoechst 33342 dye. bisbenzimide ethoxide trihydrochloride 92-105 ATP binding cassette subfamily B member 1 Homo sapiens 25-29 19764648-8 2009 Fluorescent dyes, Hoechst 33342 and PI, produced diffuse staining of the nuclei in slowly proliferating cells of the SC1 and SC2 lines. bisbenzimide ethoxide trihydrochloride 18-31 transcription factor 19 Homo sapiens 117-120 19764648-8 2009 Fluorescent dyes, Hoechst 33342 and PI, produced diffuse staining of the nuclei in slowly proliferating cells of the SC1 and SC2 lines. bisbenzimide ethoxide trihydrochloride 18-31 trans-2,3-enoyl-CoA reductase Homo sapiens 125-128 18955112-5 2008 Here, we report that Hoechst 33342, a cell-permeable fluorescent probe for staining DNA and nuclei, also detects Abeta plaques in APP Tg mouse. bisbenzimide ethoxide trihydrochloride 21-34 amyloid beta (A4) precursor protein Mus musculus 113-118 18930752-9 2008 Functional expression of MDR1 and BCRP at the apical membrane was also demonstrated using a Hoechst 33342 dye. bisbenzimide ethoxide trihydrochloride 92-105 BCR pseudogene 1 Homo sapiens 34-38 18678495-5 2008 The biological activity data were determined by our new Hoechst 33342 assay that has been transferred from P-gp to BCRP overexpressing cells. bisbenzimide ethoxide trihydrochloride 56-69 ATP binding cassette subfamily B member 1 Homo sapiens 107-111 18820285-2 2008 In the intrinsic drug resistance of hepatocellular carcinoma (HCC), the role of ABCG2 is closely associated with "side population (SP)", a minor subset of cancer stem-like cells with unique capacity to extrude lipophilic dye Hoechst 33342 and many chemotherapeutic agents. bisbenzimide ethoxide trihydrochloride 225-238 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 80-85 18799806-3 2008 The inhibitory effect of GF120918 was verified in Madin-Darby canine kidney II cells stably expressing rat Abcg2 with Hoechst 33342 and nitrofurantoin flux in Transwells. bisbenzimide ethoxide trihydrochloride 118-131 ATP binding cassette subfamily G member 2 Rattus norvegicus 107-112 18678495-5 2008 The biological activity data were determined by our new Hoechst 33342 assay that has been transferred from P-gp to BCRP overexpressing cells. bisbenzimide ethoxide trihydrochloride 56-69 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 115-119 18182157-5 2008 By measuring both GFP and Hoechst 33342 dye fluorescence in HEK-293 cells, we provide evidence that a real-time transport assay can be reliably applied to identify ABCG2 substrates, transport modulators, as well as to monitor the cellular functions of this multidrug transporter protein. bisbenzimide ethoxide trihydrochloride 26-39 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 164-169 18656616-4 2008 METHODS: Huh7 cells were sorted using Hoechst 33342 and Pyronin Y. bisbenzimide ethoxide trihydrochloride 38-51 MIR7-3 host gene Homo sapiens 9-13 17978814-5 2008 Hoechst 33342 dye efflux from primary human proximal tubule cells was significantly reduced by the BCRP/ABCG2 inhibitors fumitremorgin C and nelfinavir. bisbenzimide ethoxide trihydrochloride 0-13 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 99-103 18024518-4 2008 The efflux of DAPI and Hoechst 33342 from E. coli cells expressing SmdAB was observed, and the efflux activities were inhibited by sodium o-vanadate, which is a well-known ATPase inhibitor. bisbenzimide ethoxide trihydrochloride 23-36 ATPase Escherichia coli 172-178 17978814-5 2008 Hoechst 33342 dye efflux from primary human proximal tubule cells was significantly reduced by the BCRP/ABCG2 inhibitors fumitremorgin C and nelfinavir. bisbenzimide ethoxide trihydrochloride 0-13 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 104-109 17289934-8 2007 Flow cytometry of myometrial cells identified a myometrial Hoechst 33342 effluxing "side population" that expresses MISRII-Cre-driven YFP. bisbenzimide ethoxide trihydrochloride 59-72 anti-Mullerian hormone type 2 receptor Mus musculus 116-122 17890094-0 2007 New functional assay of P-glycoprotein activity using Hoechst 33342. bisbenzimide ethoxide trihydrochloride 54-67 ATP binding cassette subfamily B member 1 Homo sapiens 24-38 17890094-1 2007 In this study we describe a simplified, HTS-capable functional assay for the multidrug resistance (MDR) transporter P-glycoprotein (P-gp) based on its substrate Hoechst 33342. bisbenzimide ethoxide trihydrochloride 161-174 ATP binding cassette subfamily B member 1 Homo sapiens 116-130 17890094-1 2007 In this study we describe a simplified, HTS-capable functional assay for the multidrug resistance (MDR) transporter P-glycoprotein (P-gp) based on its substrate Hoechst 33342. bisbenzimide ethoxide trihydrochloride 161-174 ATP binding cassette subfamily B member 1 Homo sapiens 132-136 17535157-6 2007 In support of this mechanism, we found that expression of P-glycoprotein (CFTR"s sister protein) processing mutants in the presence of two compounds that bind to different sites (rhodamine B and Hoechst 33342) had an additive effect on maturation. bisbenzimide ethoxide trihydrochloride 195-208 ATP binding cassette subfamily B member 1 Homo sapiens 58-72 17870057-6 2007 Apoptosis, as determined by TUNEL and Hoechst 33342 staining, was accordingly less for Igf2(-/-) mice. bisbenzimide ethoxide trihydrochloride 38-51 insulin-like growth factor 2 Mus musculus 87-91 17535157-6 2007 In support of this mechanism, we found that expression of P-glycoprotein (CFTR"s sister protein) processing mutants in the presence of two compounds that bind to different sites (rhodamine B and Hoechst 33342) had an additive effect on maturation. bisbenzimide ethoxide trihydrochloride 195-208 CF transmembrane conductance regulator Homo sapiens 74-78 17584961-1 2007 Side population (SP) cells are characterized by their ability to efflux the vital dye Hoechst 33342 (Sigma-Aldrich, St. Louis, MO) due to expression of the ATP binding cassette (ABC)-dependent transporter ABCG2, and are highly enriched for stem/progenitor cell activity. bisbenzimide ethoxide trihydrochloride 86-99 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 205-210 17519960-5 2007 Herein we demonstrate a dose-dependent, reversible inhibition of ABCG2-mediated Hoechst 33342 dye efflux in primary human and murine HSC by both imatinib and nilotinib (AMN107), a novel aminopyrimidine inhibitor of BCR-ABL. bisbenzimide ethoxide trihydrochloride 80-93 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 65-70 16799018-5 2006 P2X7 cell death receptor activation was evaluated using the YO-PRO-1 assay and apoptosis (chromatin condensation and translocation of phosphatidylserine) using the Hoechst 33342 and annexin V-FITC dyes. bisbenzimide ethoxide trihydrochloride 164-177 purinergic receptor P2X 7 Homo sapiens 0-4 17131346-8 2007 Significant ABCG2-mediated extrusion of Hoechst 33342 was demonstrated in RH30 but not in RD, and was inhibited by imatinib and the specific ABCG2 inhibitor Ko143. bisbenzimide ethoxide trihydrochloride 40-53 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 12-17 17131346-8 2007 Significant ABCG2-mediated extrusion of Hoechst 33342 was demonstrated in RH30 but not in RD, and was inhibited by imatinib and the specific ABCG2 inhibitor Ko143. bisbenzimide ethoxide trihydrochloride 40-53 Rh blood group D antigen Homo sapiens 74-78 17131346-8 2007 Significant ABCG2-mediated extrusion of Hoechst 33342 was demonstrated in RH30 but not in RD, and was inhibited by imatinib and the specific ABCG2 inhibitor Ko143. bisbenzimide ethoxide trihydrochloride 40-53 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 141-146 17917153-2 2007 Moreover, a small number of cancer cells express stem cell markers, including CD133 and ATP-binding cassette transporters, by which the cells can pump out specific fluorescence dyes, such as Hoechst33342, as well as anti-cancer drugs, suggesting that either cancer cells resemble stem cells or cancers contain stem cell-like cancer cells, called "cancer stem cells (CSCs)". bisbenzimide ethoxide trihydrochloride 191-203 prominin 1 Homo sapiens 78-83 17215888-7 2006 These compounds also inhibit Pgp-mediated flipping of fluorescent lipids and transport of Hoechst 33342 and tetramethylrosamine, which occupy different subsites in the drug-binding pocket. bisbenzimide ethoxide trihydrochloride 90-103 phosphoglycolate phosphatase Homo sapiens 29-32 16804114-3 2006 When stained with Hoechst 33342 dye, the CD34(-/low) c-Kit(+)Sca-1(+)lineage marker(-) (CD34(-)KSL) cell population, highly enriched in mouse HSCs, was almost equally divided into the SP and the main population (MP) that represents non-SP cells. bisbenzimide ethoxide trihydrochloride 18-31 CD34 antigen Mus musculus 41-45 16804114-3 2006 When stained with Hoechst 33342 dye, the CD34(-/low) c-Kit(+)Sca-1(+)lineage marker(-) (CD34(-)KSL) cell population, highly enriched in mouse HSCs, was almost equally divided into the SP and the main population (MP) that represents non-SP cells. bisbenzimide ethoxide trihydrochloride 18-31 KIT proto-oncogene receptor tyrosine kinase Mus musculus 53-58 17392340-12 2007 We propose that hindrance of H369W-Gfi-1 interactions in the survivin promoter, initiated by Hoechst33342, contributes to upregulation of survivin transcription, and as a consequence, hampers Hoechst33342"s cytotoxicity. bisbenzimide ethoxide trihydrochloride 93-105 growth factor independent 1 transcriptional repressor Homo sapiens 35-40 17392340-12 2007 We propose that hindrance of H369W-Gfi-1 interactions in the survivin promoter, initiated by Hoechst33342, contributes to upregulation of survivin transcription, and as a consequence, hampers Hoechst33342"s cytotoxicity. bisbenzimide ethoxide trihydrochloride 192-204 growth factor independent 1 transcriptional repressor Homo sapiens 35-40 16978655-3 2006 Catechin and epicatechin inhibited 10 microM Abeta (25-35)-induced neuronal cell death at a concentration of 10 microM, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 222-235 amyloid beta precursor protein Rattus norvegicus 45-50 16966095-4 2006 METHODS: p53(+) and p53(-) cells were synchronized in G2 phase using Hoechst 33342 and released from synchrony in the presence or absence of 5 microM sodium arsenite. bisbenzimide ethoxide trihydrochloride 69-82 tumor protein p53 Homo sapiens 9-12 16966095-4 2006 METHODS: p53(+) and p53(-) cells were synchronized in G2 phase using Hoechst 33342 and released from synchrony in the presence or absence of 5 microM sodium arsenite. bisbenzimide ethoxide trihydrochloride 69-82 tumor protein p53 Homo sapiens 20-23 16682516-5 2006 In conclusion, the inhibition of fatty acid synthesis induced by 20 microg/ml of Hoechst 33342 is attributed to the degradation of FAS protein by activated caspases rather than by inhibition of FAS enzyme activity or FAS mRNA synthesis. bisbenzimide ethoxide trihydrochloride 81-94 fatty acid synthase Homo sapiens 131-134 16497458-4 2006 SCR, over a concentration range of 10-50 microg/ml, inhibited 10 microM Abeta (25-35)-induced neuronal cell death, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 217-230 amyloid beta precursor protein Rattus norvegicus 72-77 16682516-0 2006 Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes. bisbenzimide ethoxide trihydrochloride 91-104 fatty acid synthase Homo sapiens 0-19 16540898-2 2006 METHODS: SP cells were identified using flow cytometry analysis by the ability of these cells to extrude the Hoechst 33342 dye via the drug transporter BCRP1/ABCG2. bisbenzimide ethoxide trihydrochloride 109-122 BCR pseudogene 1 Homo sapiens 152-157 16682516-2 2006 Hoechst 33342-induced apoptosis in BC3H-1 myocytes was used as a model to explore intracellular changes in FAS protein (Western blot) and FAS mRNA (RT-PCR). bisbenzimide ethoxide trihydrochloride 0-13 fatty acid synthase Homo sapiens 107-110 16682516-2 2006 Hoechst 33342-induced apoptosis in BC3H-1 myocytes was used as a model to explore intracellular changes in FAS protein (Western blot) and FAS mRNA (RT-PCR). bisbenzimide ethoxide trihydrochloride 0-13 fatty acid synthase Homo sapiens 138-141 16682516-4 2006 Hoechst 33342 at 20 microg/ml reduced the concentration of FAS protein, which was followed more than 6 hr later by a reduction in FAS mRNA. bisbenzimide ethoxide trihydrochloride 0-13 fatty acid synthase Homo sapiens 59-62 16682516-4 2006 Hoechst 33342 at 20 microg/ml reduced the concentration of FAS protein, which was followed more than 6 hr later by a reduction in FAS mRNA. bisbenzimide ethoxide trihydrochloride 0-13 fatty acid synthase Homo sapiens 130-133 16540898-2 2006 METHODS: SP cells were identified using flow cytometry analysis by the ability of these cells to extrude the Hoechst 33342 dye via the drug transporter BCRP1/ABCG2. bisbenzimide ethoxide trihydrochloride 109-122 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 158-163 16344902-6 2005 The morphological data of caspase-3 immunofluorocytochemistry double staining with hoechst 33342 indicated that apoptotic nuclei were identified as nuclei with chromatin condensation and nuclear fragmentation, and that caspase-3 active p17 subunit co-existed in PC12 cells treated with roscovitine 50 micromol/L for 4 h. The number of the caspase-3 positive cells increased significantly to about 42%, as compared with the normal control (P<0.001). bisbenzimide ethoxide trihydrochloride 83-96 caspase 3 Rattus norvegicus 26-35 16793730-10 2006 The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. bisbenzimide ethoxide trihydrochloride 169-182 enolase 2 Homo sapiens 60-82 16793730-10 2006 The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. bisbenzimide ethoxide trihydrochloride 169-182 enolase 2 Homo sapiens 84-87 16793730-10 2006 The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. bisbenzimide ethoxide trihydrochloride 169-182 glial fibrillary acidic protein Homo sapiens 93-124 16793730-10 2006 The presence of the neural cell markers neurofilament (NF), neuro-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) was observed in approximately 10% of Hoechst 33342-labeled cells. bisbenzimide ethoxide trihydrochloride 169-182 glial fibrillary acidic protein Homo sapiens 126-130 16344902-6 2005 The morphological data of caspase-3 immunofluorocytochemistry double staining with hoechst 33342 indicated that apoptotic nuclei were identified as nuclei with chromatin condensation and nuclear fragmentation, and that caspase-3 active p17 subunit co-existed in PC12 cells treated with roscovitine 50 micromol/L for 4 h. The number of the caspase-3 positive cells increased significantly to about 42%, as compared with the normal control (P<0.001). bisbenzimide ethoxide trihydrochloride 83-96 caspase 3 Rattus norvegicus 219-228 16344902-6 2005 The morphological data of caspase-3 immunofluorocytochemistry double staining with hoechst 33342 indicated that apoptotic nuclei were identified as nuclei with chromatin condensation and nuclear fragmentation, and that caspase-3 active p17 subunit co-existed in PC12 cells treated with roscovitine 50 micromol/L for 4 h. The number of the caspase-3 positive cells increased significantly to about 42%, as compared with the normal control (P<0.001). bisbenzimide ethoxide trihydrochloride 83-96 caspase 3 Rattus norvegicus 219-228 16051989-5 2005 The ABCG2-expressing cell population was isolated as a side population (SP) by cell sorting after exposure to Hoechst 33342 dye. bisbenzimide ethoxide trihydrochloride 110-123 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 4-9 16229491-3 2005 Two drug binding sites within P-glycoprotein have been described which interact allosterically, the H-site (binds Hoechst 33342) and the R-site (binds rhodamine 123); however, the structural and functional relationship between the various binding sites appears complex. bisbenzimide ethoxide trihydrochloride 114-127 ATP binding cassette subfamily B member 1 Homo sapiens 30-44 15811951-1 2005 When cell populations are incubated with the DNA-binding dye Hoechst 33342 and subjected to flow cytometry analysis for Hoechst 33342 emissions, active efflux of the dye by the ABCG2/BCRP1 transporter causes certain cells to appear as a segregated cohort, known as a side population (SP). bisbenzimide ethoxide trihydrochloride 61-74 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 177-182 16081165-5 2005 Analysis of neurons stained with Hoechst 33342 demonstrated the anti-apoptotic effects of 5-HT1A agonists and implicated the involvement of the PI-3K pathway and possibly the MAPKK pathway with the protective effects of these drugs. bisbenzimide ethoxide trihydrochloride 33-46 5-hydroxytryptamine receptor 1A Homo sapiens 90-96 15838659-9 2005 Decreased transport of Hoechst 33342 was observed in Sf9 cells expressing V12M ABCG2; however, this was not true in HEK-293 cells expressing V12M ABCG2. bisbenzimide ethoxide trihydrochloride 23-36 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 79-84 16105665-8 2005 The mdr1 gene was functional since expanded cells effluxed Hoechst 33342 and Rh123 dyes. bisbenzimide ethoxide trihydrochloride 59-72 ATP binding cassette subfamily B member 1 Homo sapiens 4-8 15811951-1 2005 When cell populations are incubated with the DNA-binding dye Hoechst 33342 and subjected to flow cytometry analysis for Hoechst 33342 emissions, active efflux of the dye by the ABCG2/BCRP1 transporter causes certain cells to appear as a segregated cohort, known as a side population (SP). bisbenzimide ethoxide trihydrochloride 61-74 BCR pseudogene 1 Homo sapiens 183-188 15811951-1 2005 When cell populations are incubated with the DNA-binding dye Hoechst 33342 and subjected to flow cytometry analysis for Hoechst 33342 emissions, active efflux of the dye by the ABCG2/BCRP1 transporter causes certain cells to appear as a segregated cohort, known as a side population (SP). bisbenzimide ethoxide trihydrochloride 120-133 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 177-182 15811951-1 2005 When cell populations are incubated with the DNA-binding dye Hoechst 33342 and subjected to flow cytometry analysis for Hoechst 33342 emissions, active efflux of the dye by the ABCG2/BCRP1 transporter causes certain cells to appear as a segregated cohort, known as a side population (SP). bisbenzimide ethoxide trihydrochloride 120-133 BCR pseudogene 1 Homo sapiens 183-188 15680252-2 2005 Acetaldehyde induced rapid and transient (15 min) activation of p42/44 MAPK followed by activation of JNK, which remained above control up to 1 h. Ethanol activated JNK for up to 4 h. Both ethanol and acetaldehyde caused apoptosis as determined by DNA fragmentation, caspase-3 activation and 2"[4-ethoxyphenyl]-5-[4-methyl-piperazinyl]-2,5"-bi-1H-benzimidazole (Hoechst 33342) staining. bisbenzimide ethoxide trihydrochloride 362-375 mitogen activated protein kinase 1 Rattus norvegicus 64-75 15528299-4 2005 Hoechst 33342 staining and flow cytometric analysis confirmed the induction of apoptosis in granulosa cells by 100 ng/ml rFasL in the presence of interferon-gamma, which was blocked by the concomitant addition of an NO donor, S-nitroso-N-acetylpenicillamine. bisbenzimide ethoxide trihydrochloride 0-13 Fas ligand Rattus norvegicus 121-126 15528299-4 2005 Hoechst 33342 staining and flow cytometric analysis confirmed the induction of apoptosis in granulosa cells by 100 ng/ml rFasL in the presence of interferon-gamma, which was blocked by the concomitant addition of an NO donor, S-nitroso-N-acetylpenicillamine. bisbenzimide ethoxide trihydrochloride 0-13 interferon gamma Homo sapiens 146-162 15857213-5 2005 To verify induction of apoptosis by the SNL glycoprotein, we performed DNA fragmentation and nuclear staining assays using ethidium bromide and bisbenzamide H33342. bisbenzimide ethoxide trihydrochloride 157-163 fascin actin-bundling protein 1 Homo sapiens 40-43 15680252-2 2005 Acetaldehyde induced rapid and transient (15 min) activation of p42/44 MAPK followed by activation of JNK, which remained above control up to 1 h. Ethanol activated JNK for up to 4 h. Both ethanol and acetaldehyde caused apoptosis as determined by DNA fragmentation, caspase-3 activation and 2"[4-ethoxyphenyl]-5-[4-methyl-piperazinyl]-2,5"-bi-1H-benzimidazole (Hoechst 33342) staining. bisbenzimide ethoxide trihydrochloride 362-375 mitogen-activated protein kinase 8 Rattus norvegicus 102-105 15680252-2 2005 Acetaldehyde induced rapid and transient (15 min) activation of p42/44 MAPK followed by activation of JNK, which remained above control up to 1 h. Ethanol activated JNK for up to 4 h. Both ethanol and acetaldehyde caused apoptosis as determined by DNA fragmentation, caspase-3 activation and 2"[4-ethoxyphenyl]-5-[4-methyl-piperazinyl]-2,5"-bi-1H-benzimidazole (Hoechst 33342) staining. bisbenzimide ethoxide trihydrochloride 362-375 mitogen-activated protein kinase 8 Rattus norvegicus 165-168 15389547-7 2004 We also report that in the presence of FGF6, the minor (0.5-2%) subpopulation of cells actively excluding Hoechst 33342 in a verapamil-dependent manner (SP phenotype) was increased to 15-20% and the expression of the mdr1a gene (but not mdr1b) was upregulated by 400-fold. bisbenzimide ethoxide trihydrochloride 106-119 fibroblast growth factor 6 Mus musculus 39-43 15797262-9 2005 Up-regulated HO-1 expression was accompanied by increase of the numbers of Hoechst-33342 positive MSCs, the reduction of infarct size, and the improvement of cardiac function. bisbenzimide ethoxide trihydrochloride 75-88 heme oxygenase 1 Homo sapiens 13-17 15516692-2 2005 Hematopoietic cells can be highly enriched for repopulating ability based upon the efflux of the fluorescent Hoechst 33342 dye by sorting for SP (side population) cells, a phenotype attributed to expression of ABCG2, a member of the ABC transporter superfamily. bisbenzimide ethoxide trihydrochloride 109-122 ATP binding cassette subfamily G member 2 Macaca mulatta 210-215 15476243-12 2004 Incubation of ECs with these anti-Hsp60 antibodies induced apoptosis in a time- and dose-dependent manner, as determined by Hoechst 33342 dye staining of condensed nuclei and by annexin V binding to surface phosphatidylserine. bisbenzimide ethoxide trihydrochloride 124-137 heat shock protein family D (Hsp60) member 1 Homo sapiens 34-39 14522974-9 2003 By contrast, verapamil and Hoechst 33342 stimulated and inhibited, respectively, the ATPase activity of the MTS-rhodamine-treated mutant F343C. bisbenzimide ethoxide trihydrochloride 27-40 dynein axonemal heavy chain 8 Homo sapiens 85-91 15389434-9 2004 Furthermore, the most quiescent CD34 + cells isolated on the basis of low Hoechst 33342 (Ho) and rhodamine 123 (Rho) staining (Ho Low /Rho Low ) were highly enriched in the CXCR4 Low/- cell population. bisbenzimide ethoxide trihydrochloride 74-87 C-X-C chemokine receptor type 4 Papio anubis 173-178 15571275-4 2004 8226/MR cells displayed a larger cellular efflux rate of the BCRP substrate Hoechst 33342, as compared to the wildtype cells. bisbenzimide ethoxide trihydrochloride 76-89 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 61-65 15446579-4 2004 Within caspase 3-positive neurons, fragmented nuclei were co-localized using Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 77-90 caspase 3 Mus musculus 7-16 15558489-7 2004 ABCG2 transport activity excludes the DNA dye Hoechst 33342, allowing the isolation of the ocular stem cells by flow cytometry, as a unique cohort known as a side "side population". bisbenzimide ethoxide trihydrochloride 46-59 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 0-5 15047700-8 2004 Loss of BCRP expression in MCF-7/LF and MCF-7/MR-LF cells resulted in the following: (a) a prominent decrease in the efflux of Hoechst 33342, a BCRP substrate; (b) an approximately 2-fold increase in MR accumulation as revealed by flow cytometry; this was accompanied by a 2.5- and approximately 84-fold increased MR sensitivity in these cell lines, respectively. bisbenzimide ethoxide trihydrochloride 127-140 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 8-12 15047700-8 2004 Loss of BCRP expression in MCF-7/LF and MCF-7/MR-LF cells resulted in the following: (a) a prominent decrease in the efflux of Hoechst 33342, a BCRP substrate; (b) an approximately 2-fold increase in MR accumulation as revealed by flow cytometry; this was accompanied by a 2.5- and approximately 84-fold increased MR sensitivity in these cell lines, respectively. bisbenzimide ethoxide trihydrochloride 127-140 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 144-148 15067695-0 2004 Bidirectional transport of rhodamine 123 and Hoechst 33342, fluorescence probes of the binding sites on P-glycoprotein, across MDCK-MDR1 cell monolayers. bisbenzimide ethoxide trihydrochloride 45-58 PGP Canis lupus familiaris 104-118 15067695-0 2004 Bidirectional transport of rhodamine 123 and Hoechst 33342, fluorescence probes of the binding sites on P-glycoprotein, across MDCK-MDR1 cell monolayers. bisbenzimide ethoxide trihydrochloride 45-58 ATP binding cassette subfamily B member 1 Homo sapiens 132-136 15067695-3 2004 The P-gp inhibitor GF-120918 could significantly reduce the polarized efflux of both rhodamine 123 and Hoechst 33342. bisbenzimide ethoxide trihydrochloride 103-116 PGP Canis lupus familiaris 4-8 15067695-4 2004 Rhodamine 123 appeared to "stimulate" the polarized efflux of Hoechst 33342 across MDCK-MDR1 cell monolayers. bisbenzimide ethoxide trihydrochloride 62-75 ATP binding cassette subfamily B member 1 Canis lupus familiaris 83-92 15067695-6 2004 The uptake characteristics of rhodamine 123 and Hoechst 33342 in MDCK-MDR1 cells were measured in the absence and presence of GF-120918 and known P-gp substrates (Hoechst 33342, rhodamine 123, and daunorubicin). bisbenzimide ethoxide trihydrochloride 48-61 ATP binding cassette subfamily B member 1 Canis lupus familiaris 65-74 15067695-7 2004 The uptake of rhodamine 123 and Hoechst 33342 in MDCK-MDR1 cells was enhanced more than twofold by inclusion of GF-120918 (2 microM) in the incubation medium. bisbenzimide ethoxide trihydrochloride 32-45 ATP binding cassette subfamily B member 1 Canis lupus familiaris 49-58 15067695-13 2004 In conclusion, these bidirectional transport data indicate that rhodamine 123 and Hoechst 33342 are excellent substrates of P-gp in MDCK-MDR1 cells. bisbenzimide ethoxide trihydrochloride 82-95 PGP Canis lupus familiaris 124-128 15067695-13 2004 In conclusion, these bidirectional transport data indicate that rhodamine 123 and Hoechst 33342 are excellent substrates of P-gp in MDCK-MDR1 cells. bisbenzimide ethoxide trihydrochloride 82-95 ATP binding cassette subfamily B member 1 Canis lupus familiaris 132-141 15067695-14 2004 The ability of Hoechst 33342 to partially inhibit the polarized efflux of rhodamine 123 is consistent with these substrates binding to the same site on P-gp. bisbenzimide ethoxide trihydrochloride 15-28 PGP Canis lupus familiaris 152-156 15067695-15 2004 In contrast, the ability of rhodamine 123 to apparently "stimulate" the efflux of Hoechst 33342 in both the transport and uptake experiments suggests the substrates might bind to different sites on P-gp. bisbenzimide ethoxide trihydrochloride 82-95 PGP Canis lupus familiaris 198-202 15067695-17 2004 Thus, the use of Hoechst 33342 to probe the binding sites on a membrane-bound protein such as P-gp might be problematic. bisbenzimide ethoxide trihydrochloride 17-30 PGP Canis lupus familiaris 94-98 15086165-10 2004 The blood perfusion data of the tumor, represented by number of Hoechst 33342 labeled cells, showed an inverse correlation with the expression of VEGF mRNA in tumor tissue (P<0.05). bisbenzimide ethoxide trihydrochloride 64-77 vascular endothelial growth factor A Rattus norvegicus 146-150 14699605-1 2004 BACKGROUND: Discrimination of stem cells with flow cytometric analysis of Hoechst 33342 efflux by the ABCG2 transporter (termed the Hoechst side population, or SP technique) is a valuable methodology for identifying bone marrow progenitors enriched with stem cells. bisbenzimide ethoxide trihydrochloride 74-87 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 102-107 14769356-4 2003 Rats were killed between day 0 and day 14 after compression and apoptosis of SGCs was evaluated quantitatively as well as qualitatively by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining, anti-activated caspase-3 immunostaining, Hoechst 33342 staining, and electron microscopy. bisbenzimide ethoxide trihydrochloride 295-308 DNA nucleotidylexotransferase Rattus norvegicus 178-181 14612912-3 2003 By flow cytometry, mitoxantrone, BODIPY-prazosin, and Hoechst 33342 were found to be substrates of all ABCG2 proteins, while rhodamine 123, daunorubicin, and LysoTracker Green were transported only by mutant ABCG2. bisbenzimide ethoxide trihydrochloride 54-67 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 103-108 14576842-12 2003 BCRP expression has also been demonstrated in pluripotential "side population" stem cells, responsible for the characteristic ability of these cells to exclude Hoechst 33342 dye, and possibly for the maintenance of the stem cell phenotype. bisbenzimide ethoxide trihydrochloride 160-173 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 0-4 12662438-1 2003 The rapid efflux of the fluorescent DNA-binding dye Hoechst 33342 identifies a rare, so-called side population (SP), which rapidly expels the dye, can reconstitute the bone marrow (BM) of lethally irradiated mice, and has proven negative for most lineage markers including CD34. bisbenzimide ethoxide trihydrochloride 52-65 CD34 antigen Mus musculus 273-277 14579240-3 2003 ABCG2 expression is associated with the side population (SP) phenotype of Hoechst 33342 efflux. bisbenzimide ethoxide trihydrochloride 74-87 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 0-5 12576187-5 2003 Two of them, active caspase 3 and c-Jun/AP-1 (N) displayed a similar time course and reached a maximum after 24 h of exposure, 24 h ahead of both PI uptake and Hoechst 33342 staining, which together displayed similar time profiles and a close correlation. bisbenzimide ethoxide trihydrochloride 160-173 caspase 3 Homo sapiens 20-29 12576187-5 2003 Two of them, active caspase 3 and c-Jun/AP-1 (N) displayed a similar time course and reached a maximum after 24 h of exposure, 24 h ahead of both PI uptake and Hoechst 33342 staining, which together displayed similar time profiles and a close correlation. bisbenzimide ethoxide trihydrochloride 160-173 Jun proto-oncogene, AP-1 transcription factor subunit Homo sapiens 34-39 12559966-10 2003 The change in nucleolin in Saos-2 cells induced to undergo apoptosis was examined by an immunocytochemical procedure using the anti-nucleolin antibody and Hoechst 33342. bisbenzimide ethoxide trihydrochloride 155-168 nucleolin Homo sapiens 14-23 12559966-12 2003 The dual-exposure view of Hoechst 33342 and anti-nucleolin staining cells confirmed that nucleolin had disappeared from the apoptotic nuclei of Saos-2. bisbenzimide ethoxide trihydrochloride 26-39 nucleolin Homo sapiens 89-98 12393870-2 2002 Here we show that the FFA, oleic acid, increased apoptosis 16-fold in the pancreatic beta-cell line, INS-1, over a 18-h period as assessed by Hoechst 33342/propidium iodide staining and caspase-3 and -9 activation, with negligible necrosis. bisbenzimide ethoxide trihydrochloride 142-155 forkhead box M1 Homo sapiens 101-106 12374800-7 2002 The wild-type ABCG2 and its variants, R482G and R482T, showed characteristically different drug and dye transport activities; mitoxantrone and Hoechst 33342 were transported by all transporters, whereas rhodamine 123 was only pumped by the R482G and R482T mutants. bisbenzimide ethoxide trihydrochloride 143-156 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 14-19 12461294-6 2002 Culture of G(0) CD34(+) cells isolated based on Hoechst 33342/PyroninY staining with Tpo, SCF and FL for 48 hrs, results in significantly elevated Survivin mRNA and protein levels. bisbenzimide ethoxide trihydrochloride 48-61 CD34 molecule Homo sapiens 16-20 12374627-4 2002 Ferryl Mb formation correlated with the induction of apoptosis as indicated by morphological criteria, caspase 3 activation, phosphatidylserine (PS) externalization, and nuclear condensation by Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 194-207 myoglobin Bos taurus 7-9 12205677-6 2002 Furthermore, we demonstrated that, at all stages examined, the "side population" (SP), defined as the Hoechst 33342 low/negative fraction, which is known to be a stem cell-enriched population in bone marrow, was also enriched for Notch1-positive immature neural cells (about 60%) from the developing striatum. bisbenzimide ethoxide trihydrochloride 102-115 notch 1 Mus musculus 230-236 12417095-7 2002 Hoechst 33342-induced apoptosis is associated with disruption of TATA box binding protein/TATA box complexes, replication protein A/single-stranded DNA complexes, topoisomerase I/DNA cleavable complexes and with an increased intracellular concentration of E2F-1 transcription factor and nitric oxide concentration. bisbenzimide ethoxide trihydrochloride 0-13 TATA-box binding protein Homo sapiens 65-89 12373335-13 2002 Whereas the p53-positive endothelial cells underwent programmed cell death as demonstrated by M30, ISEL and Hoechst 33342, some fibroblasts seemed to accumulate the p53 antibody, but this did not induce apoptotic cascades. bisbenzimide ethoxide trihydrochloride 108-121 tumor protein p53 Homo sapiens 12-15 12461294-6 2002 Culture of G(0) CD34(+) cells isolated based on Hoechst 33342/PyroninY staining with Tpo, SCF and FL for 48 hrs, results in significantly elevated Survivin mRNA and protein levels. bisbenzimide ethoxide trihydrochloride 48-61 thrombopoietin Homo sapiens 85-88 12054520-5 2002 Recently it was shown that the exclusion of the Hoechst 33342 dye, which defines the pluripotential side population (SP) of hematopoietic stem cells, is mediated by the ATP-binding cassette transporter, ABCG2. bisbenzimide ethoxide trihydrochloride 48-61 ATP binding cassette subfamily A member 4 Homo sapiens 169-201 12046073-8 2002 The blood perfusion data of the tumor, represented by the number of Hoechst 33342 labeled cells, showed a good linear inverse correlation with the serum VEGF level (r=-0.606, P<0.05) and the expression of VEGF mRNA in the tumor tissue ( r =-0.338, P<0.01). bisbenzimide ethoxide trihydrochloride 68-81 vascular endothelial growth factor A Rattus norvegicus 153-157 12160837-1 2002 OBJECTIVE: Several lines of evidence suggest that expression of two ABC transporters (Abcg2/Bcrp1 and mdr-1a/b) and the related abilities to efflux Hoechst 33342 (Hst) and Rhodamine-123 (Rho) are features of primitive hematopoietic cells in adult bone marrow. bisbenzimide ethoxide trihydrochloride 148-161 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 86-91 12160837-1 2002 OBJECTIVE: Several lines of evidence suggest that expression of two ABC transporters (Abcg2/Bcrp1 and mdr-1a/b) and the related abilities to efflux Hoechst 33342 (Hst) and Rhodamine-123 (Rho) are features of primitive hematopoietic cells in adult bone marrow. bisbenzimide ethoxide trihydrochloride 148-161 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 92-97 12160837-1 2002 OBJECTIVE: Several lines of evidence suggest that expression of two ABC transporters (Abcg2/Bcrp1 and mdr-1a/b) and the related abilities to efflux Hoechst 33342 (Hst) and Rhodamine-123 (Rho) are features of primitive hematopoietic cells in adult bone marrow. bisbenzimide ethoxide trihydrochloride 148-161 ATP-binding cassette, sub-family B (MDR/TAP), member 1A Mus musculus 102-108 12054520-5 2002 Recently it was shown that the exclusion of the Hoechst 33342 dye, which defines the pluripotential side population (SP) of hematopoietic stem cells, is mediated by the ATP-binding cassette transporter, ABCG2. bisbenzimide ethoxide trihydrochloride 48-61 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 203-208 11926837-0 2002 Proximity of bound Hoechst 33342 to the ATPase catalytic sites places the drug binding site of P-glycoprotein within the cytoplasmic membrane leaflet. bisbenzimide ethoxide trihydrochloride 19-32 dynein axonemal heavy chain 8 Homo sapiens 40-46 11926837-0 2002 Proximity of bound Hoechst 33342 to the ATPase catalytic sites places the drug binding site of P-glycoprotein within the cytoplasmic membrane leaflet. bisbenzimide ethoxide trihydrochloride 19-32 ATP binding cassette subfamily B member 1 Homo sapiens 95-109 11926837-5 2002 The fluorescent dye Hoechst 33342 (H33342), a high-affinity P-glycoprotein substrate, bound to the transporter and acted as a FRET donor. bisbenzimide ethoxide trihydrochloride 20-33 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 11926837-5 2002 The fluorescent dye Hoechst 33342 (H33342), a high-affinity P-glycoprotein substrate, bound to the transporter and acted as a FRET donor. bisbenzimide ethoxide trihydrochloride 35-41 ATP binding cassette subfamily B member 1 Homo sapiens 60-74 11926837-6 2002 H33342 showed greatly enhanced fluorescence emission when bound to P-glycoprotein, together with a substantial blue shift, indicating that the drug binding site is located in a nonpolar environment. bisbenzimide ethoxide trihydrochloride 0-6 ATP binding cassette subfamily B member 1 Homo sapiens 67-81 11926837-8 2002 H33342 fluorescence was highly quenched when bound to NBD-labeled P-glycoprotein relative to unlabeled protein, indicating that FRET takes place from the bound dye to NBD. bisbenzimide ethoxide trihydrochloride 0-6 ATP binding cassette subfamily B member 1 Homo sapiens 66-80 11897053-1 2002 A microplate screening method has been developed to evaluate the effects of test agents on the accumulation of the fluorescent P-glycoprotein (Pgp) substrates Hoechst 33342, rhodamine 123, and rhodamine 6G in multidrug-resistant (MDR) breast cancer cells that overexpress Pgp. bisbenzimide ethoxide trihydrochloride 159-172 ATP binding cassette subfamily B member 1 Homo sapiens 127-141 11895771-4 2002 Telomere length was measured by an in-gel hybridization technique, X-inactivation ratios were measured by the human androgen receptor assay, and cell cycle status was determined by flow cytometric analysis of pyronin Y- and Hoechst 33342-stained CD34(+)CD90(+) and CD34(+)CD90(-) marrow cells. bisbenzimide ethoxide trihydrochloride 224-237 CD34 molecule Homo sapiens 246-250 11956966-5 2002 After treatment with SIN-1, the apoptotic DNA damage in the cells was quantified by a single cell gel electrophoresis (comet) assay and by staining with Hoechst 33342. bisbenzimide ethoxide trihydrochloride 153-166 MAPK associated protein 1 Homo sapiens 21-26 11897053-1 2002 A microplate screening method has been developed to evaluate the effects of test agents on the accumulation of the fluorescent P-glycoprotein (Pgp) substrates Hoechst 33342, rhodamine 123, and rhodamine 6G in multidrug-resistant (MDR) breast cancer cells that overexpress Pgp. bisbenzimide ethoxide trihydrochloride 159-172 ATP binding cassette subfamily B member 1 Homo sapiens 143-146 11781231-0 2002 The ABCG2 transporter is an efficient Hoechst 33342 efflux pump and is preferentially expressed by immature human hematopoietic progenitors. bisbenzimide ethoxide trihydrochloride 38-51 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 4-9 14601472-6 2002 Hoechst 33342 demonstrated the highest toxicity with CD34+ cells, disqualifying this dye for transplantation purposes. bisbenzimide ethoxide trihydrochloride 0-13 CD34 molecule Homo sapiens 53-57 11389030-0 2001 Hoechst 33342 efflux identifies a subpopulation of cytogenetically normal CD34(+)CD38(-) progenitor cells from patients with acute myeloid leukemia. bisbenzimide ethoxide trihydrochloride 0-13 CD34 molecule Homo sapiens 74-78 11707575-4 2001 A dramatic stimulation of P-gp activity against Adr and Rho-123 by the identified compounds was accompanied by suppression of P-gp-mediated efflux of other substrates, such as Taxol (paclitaxel) or Hoechst 33342, indicating that they act as modulators of substrate specificity of P-gp. bisbenzimide ethoxide trihydrochloride 198-211 phosphoglycolate phosphatase Mus musculus 26-30 11707575-4 2001 A dramatic stimulation of P-gp activity against Adr and Rho-123 by the identified compounds was accompanied by suppression of P-gp-mediated efflux of other substrates, such as Taxol (paclitaxel) or Hoechst 33342, indicating that they act as modulators of substrate specificity of P-gp. bisbenzimide ethoxide trihydrochloride 198-211 phosphoglycolate phosphatase Mus musculus 126-130 11707575-4 2001 A dramatic stimulation of P-gp activity against Adr and Rho-123 by the identified compounds was accompanied by suppression of P-gp-mediated efflux of other substrates, such as Taxol (paclitaxel) or Hoechst 33342, indicating that they act as modulators of substrate specificity of P-gp. bisbenzimide ethoxide trihydrochloride 198-211 phosphoglycolate phosphatase Mus musculus 126-130 11573944-2 2001 We used gel mobility shift, super gel mobility shift, and Western blot to determine the fate of RPA during Hoechst 33342-induced apoptosis in HL-60 cells. bisbenzimide ethoxide trihydrochloride 107-120 replication protein A1 Homo sapiens 96-99 11573944-5 2001 After the treatment of HL-60 cells with 15 microg/ml Hoechst 33342 for 3 h, the bands of RPA/oligo(dT)(30) complexes were decreased and bands of the lowest molecular weight protein/oligo(dT)(30) complexes were significantly increased when compared to the control group. bisbenzimide ethoxide trihydrochloride 53-66 replication protein A1 Homo sapiens 89-92 11573944-7 2001 Western blotting results showed that both RPA-32 and RPA-70 were decreased significantly in a time-dependent manner after 1 h of incubation with Hoechst 33342. bisbenzimide ethoxide trihydrochloride 145-158 replication protein A2 Homo sapiens 42-48 11573944-7 2001 Western blotting results showed that both RPA-32 and RPA-70 were decreased significantly in a time-dependent manner after 1 h of incubation with Hoechst 33342. bisbenzimide ethoxide trihydrochloride 145-158 replication protein A1 Homo sapiens 53-59 11573944-8 2001 These results demonstrate that in HL-60 cells, Hoechst 33342-induced apoptosis is associated with a rapid loss of the binding capacity of RPA to oligo(dT)(30) as well as immunoactive RPA-70 and RPA-32. bisbenzimide ethoxide trihydrochloride 47-60 replication protein A1 Homo sapiens 138-141 11573944-8 2001 These results demonstrate that in HL-60 cells, Hoechst 33342-induced apoptosis is associated with a rapid loss of the binding capacity of RPA to oligo(dT)(30) as well as immunoactive RPA-70 and RPA-32. bisbenzimide ethoxide trihydrochloride 47-60 replication protein A1 Homo sapiens 183-189 11573944-8 2001 These results demonstrate that in HL-60 cells, Hoechst 33342-induced apoptosis is associated with a rapid loss of the binding capacity of RPA to oligo(dT)(30) as well as immunoactive RPA-70 and RPA-32. bisbenzimide ethoxide trihydrochloride 47-60 replication protein A2 Homo sapiens 194-200 11801536-0 2002 The multidrug resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 33342 and is overexpressed in hematopoietic stem cells. bisbenzimide ethoxide trihydrochloride 89-102 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 37-42 11801536-0 2002 The multidrug resistance transporter ABCG2 (breast cancer resistance protein 1) effluxes Hoechst 33342 and is overexpressed in hematopoietic stem cells. bisbenzimide ethoxide trihydrochloride 89-102 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 44-78 11801536-6 2002 MCF-7/MitoR cells as well as MCF-7/ABCG2 cells demonstrated lower levels of Hoechst 33342 uptake compared with the parental MCF-7 cells. bisbenzimide ethoxide trihydrochloride 76-89 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 35-40 11801536-9 2002 These results suggest that Hoechst 33342 is a substrate for the ABCG2 transporter and that ABCG2/Bcrp1 expression may serve as a marker for hematopoietic stem cells in hematopoietic cells. bisbenzimide ethoxide trihydrochloride 27-40 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 64-69 11389030-0 2001 Hoechst 33342 efflux identifies a subpopulation of cytogenetically normal CD34(+)CD38(-) progenitor cells from patients with acute myeloid leukemia. bisbenzimide ethoxide trihydrochloride 0-13 CD38 molecule Homo sapiens 81-85 11151061-0 2001 Hoechst 33342-induced apoptosis is associated with intracellular accumulation of E2F-1 protein in BC3H-1 myocytes and HL-60 cells. bisbenzimide ethoxide trihydrochloride 0-13 E2F transcription factor 1 Mus musculus 81-86 11313796-6 2001 Human hematopoietic cells transduced with SF91m3 reliably express MDR1 before and after passage through NOD/SCID mice, as shown by quantitative PCR and efflux assays with rhodamine 123 or Hoechst 33342. bisbenzimide ethoxide trihydrochloride 188-201 ATP binding cassette subfamily B member 1 Homo sapiens 66-70 11151061-3 2001 OBJECTIVE: To determine if Hoechst 33342 or Hoechst 33258 treatment of BC3H-1 myocytes or HL-60 cells is associated with the intracellular accumulation of the nuclear transcription factor E2F-1, known to induce apoptosis. bisbenzimide ethoxide trihydrochloride 27-40 E2F transcription factor 1 Homo sapiens 188-193 11151061-9 2001 Hoechst 33342 treatment increased the concentration of E2F-1 protein after a 3-hour incubation in both cell lines. bisbenzimide ethoxide trihydrochloride 0-13 E2F transcription factor 1 Homo sapiens 55-60 11151061-10 2001 CONCLUSION: Hoechst 33342-induced apoptosis is associated with intracellular accumulation of E2F-1 protein, another step in this specific apoptotic pathway. bisbenzimide ethoxide trihydrochloride 12-25 E2F transcription factor 1 Homo sapiens 93-98 12050821-2 2001 Detection of apoptotic peak by flow cytometry, fluorescent microscope observation of chromatin condensation with double staining of PI and Hoechst33342 and DNA ladder analysis all demonstrated that CsA obviously enhanced the apoptosis of HL-60 cells induced by EGTA, while highly expressed Bcl-2 completely blocked it. bisbenzimide ethoxide trihydrochloride 139-151 ERCC excision repair 8, CSA ubiquitin ligase complex subunit Homo sapiens 198-201 11015205-6 2000 Their potencies in inhibiting cell-free transcription and endogenous c-fos expression in NIH3T3 cells, however, were chromomycin > nogalamycin > Hoechst 33342. bisbenzimide ethoxide trihydrochloride 151-164 FBJ osteosarcoma oncogene Mus musculus 69-74 10234429-8 1998 We found that the low concentration of Ho342 (10 microM) recommended for isolating HSC significantly inhibited the clonogenecity of human erythroid progenitors (BFU-E). bisbenzimide ethoxide trihydrochloride 39-44 fucosyltransferase 1 (H blood group) Homo sapiens 83-86 10599977-4 1999 Fluorescent microscopic examination using Hoechst 33342 dye (Sigma, St Louis, MO) demonstrated that TNF-alpha induced time- and dose-dependent apoptotic nuclear changes in these beta cells. bisbenzimide ethoxide trihydrochloride 42-55 tumor necrosis factor Mus musculus 100-109 9920290-4 1999 MCF-7, MCF-7/LY2, and MDA-MB-231 cells all showed nuclear fragmentation in response to 100 microM A(II) when stained with Hoechst 33342 and examined by fluorescence microscopy. bisbenzimide ethoxide trihydrochloride 122-135 NLR family pyrin domain containing 3 Homo sapiens 98-103 9811569-3 1998 By comparing the HMG-I-dependent fluorescence signal with the chromatin density determined by Hoechst 33342 or propidium iodide staining, we present evidence for the existence of three HMG-I sub-populations whose contribution to the total fluorescence can be determined using a newly developed quantitative co-localization image analysis program: foci that correspond to regions of heterochromatin, intense dots located within decondensed chromatin, and a more diffuse component extending throughout the nucleoplasm. bisbenzimide ethoxide trihydrochloride 94-107 high mobility group AT-hook 1 Mus musculus 185-190 9786892-3 1998 The amount of p65 staining is measured in both the nuclei defined by Hoechst 33342 labeling and in the surrounding cytoplasmic area within a preselected number of cells/well in 96-well plates. bisbenzimide ethoxide trihydrochloride 69-82 RELA proto-oncogene, NF-kB subunit Homo sapiens 14-17 9675078-0 1998 Hoechst 33342 induces apoptosis and alters tata box binding protein/DNA complexes in nuclei from BC3H-1 myocytes. bisbenzimide ethoxide trihydrochloride 0-13 TATA box binding protein Mus musculus 43-67 9675078-6 1998 The presence of Hoechst 33342 (26.7 microM) decreased the amount of the control complex and increased the presence of lower molecular weight species suggesting degradation of nuclear TBP and/or release of other transcription factors from the complex creating a smaller sized molecular complex which retains TATA box binding capacity. bisbenzimide ethoxide trihydrochloride 16-29 TATA box binding protein Mus musculus 183-186 9537251-9 1998 Furthermore, using the fluorescent dye Hoechst 33342, an in vivo marker of perfused vessels, combined with immunochemical staining of PECAM-1 (CD31) as a marker of tumor vasculature, we found increased vascularization in the IL-2-transfected tumors. bisbenzimide ethoxide trihydrochloride 39-52 interleukin 2 Mus musculus 225-229 10980619-3 2000 Infection of NSCLC cell lines in vitro with an adenovirus expressing human IGFBP-6 under the control of a CMV promoter (Ad5CMV-BP6) reduced NSCLC cell number through activation of programmed cell death, as shown by cell staining with Hoechst 33342 or DNA end-labeling with bromodeoxyuridine triphosphate. bisbenzimide ethoxide trihydrochloride 234-247 insulin like growth factor binding protein 6 Homo sapiens 75-82 10073666-4 1999 The binding of HSF1 to the heat-shock element was measured with the gel mobility shift assay using cell extracts from Hoechst 33342-labeled heated cells sorted from G1, S and G2/M phases. bisbenzimide ethoxide trihydrochloride 118-131 heat shock factor protein 1 Cricetulus griseus 15-19 9630315-5 1998 The CD34 HSC has been already described in animals and in human Hoechst 33342 negative HSC. bisbenzimide ethoxide trihydrochloride 64-77 CD34 molecule Homo sapiens 4-8 9431998-0 1997 P-glycoprotein-mediated Hoechst 33342 transport out of the lipid bilayer. bisbenzimide ethoxide trihydrochloride 24-37 ATP binding cassette subfamily B member 1 Homo sapiens 0-14 9431998-4 1997 We explored this issue by measuring the kinetics of transport of the lipophilic P-glycoprotein substrate Hoechst 33342 by P-glycoprotein-rich plasma membrane vesicles from CH(R)B30 cells. bisbenzimide ethoxide trihydrochloride 105-118 ATP binding cassette subfamily B member 1 Homo sapiens 80-94 9431998-4 1997 We explored this issue by measuring the kinetics of transport of the lipophilic P-glycoprotein substrate Hoechst 33342 by P-glycoprotein-rich plasma membrane vesicles from CH(R)B30 cells. bisbenzimide ethoxide trihydrochloride 105-118 ATP binding cassette subfamily B member 1 Homo sapiens 122-136 9431998-7 1997 This demonstrates that P-glycoprotein removes Hoechst 33342 from the lipid membrane, where it concentrates due to its hydrophobicity. bisbenzimide ethoxide trihydrochloride 46-59 ATP binding cassette subfamily B member 1 Homo sapiens 23-37 9431999-0 1997 Extraction of Hoechst 33342 from the cytoplasmic leaflet of the plasma membrane by P-glycoprotein. bisbenzimide ethoxide trihydrochloride 14-27 ATP binding cassette subfamily B member 1 Homo sapiens 83-97 9431999-3 1997 Using Hoechst 33342 as the substrate, we have previously shown that P-glycoprotein extracts the substrate directly from the lipid bilayer [Shapiro, A. bisbenzimide ethoxide trihydrochloride 6-19 ATP binding cassette subfamily B member 1 Homo sapiens 68-82 9431999-9 1997 In this paper, we determined the leaflet of the plasma membrane from which P-glycoprotein extracts Hoechst 33342. bisbenzimide ethoxide trihydrochloride 99-112 ATP binding cassette subfamily B member 1 Homo sapiens 75-89 9431999-10 1997 The initial rate of Hoechst 33342 transport upon ATP addition to P-glycoprotein-rich inside-out plasma membrane vesicles decreased slightly with the amount of time previously elapsed for slow diffusion of Hoechst 33342 to the extracellular leaflet. bisbenzimide ethoxide trihydrochloride 20-33 ATP binding cassette subfamily B member 1 Homo sapiens 65-79 9431999-10 1997 The initial rate of Hoechst 33342 transport upon ATP addition to P-glycoprotein-rich inside-out plasma membrane vesicles decreased slightly with the amount of time previously elapsed for slow diffusion of Hoechst 33342 to the extracellular leaflet. bisbenzimide ethoxide trihydrochloride 205-218 ATP binding cassette subfamily B member 1 Homo sapiens 65-79 8660826-9 1996 Activated T cells sorted for S + G2/M DNA content following Hoechst 33342 staining were also found to be more sensitive to TCR-induced apoptosis than cells sorted for G1 DNA content. bisbenzimide ethoxide trihydrochloride 60-73 T cell receptor beta variable 20/OR9-2 (non-functional) Homo sapiens 123-126 9084433-2 1997 Both A beta(25-35)- and staurosporine-induced death of these neurons appeared to involve apoptosis, as indicated using Hoechst 33342 and terminal dUDP nick end labeling staining, whereas NMDA-induced death appeared more complex. bisbenzimide ethoxide trihydrochloride 119-132 amyloid beta precursor protein Rattus norvegicus 5-11 8615975-10 1996 Propidium iodide-Hoechst 33342 double staining confirmed that TNF-alpha-induced nuclear alterations precede permeabilization of the plasma membrane, supporting cell death in these tumor cells by apoptosis. bisbenzimide ethoxide trihydrochloride 17-30 tumor necrosis factor Homo sapiens 62-71 7608182-4 1995 We have used a fluorescent substrate of P-glycoprotein, Hoechst 33342, to measure transport activity in real-time of highly purified P-glycoprotein in a reconstituted liposome system in which the P-glycoprotein has a uniformly inside-out orientation. bisbenzimide ethoxide trihydrochloride 56-69 ATP binding cassette subfamily B member 1 Homo sapiens 40-54 8612534-11 1996 Coculturing interferon-gamma-pretreated granulosa cells with zona-free oocytes induced granulosa cell apoptosis, which was confirmed by Hoechst 33342 dye staining and terminal deoxynucleotidyl transferase-mediated deoxy-UTP-biotin nick end labeling, and the killing effect of oocytes was abolished by the addition of anti-P2, which was expected to interrupt the interaction between Fas and Fas ligand. bisbenzimide ethoxide trihydrochloride 136-149 interferon gamma Rattus norvegicus 12-28 7608182-4 1995 We have used a fluorescent substrate of P-glycoprotein, Hoechst 33342, to measure transport activity in real-time of highly purified P-glycoprotein in a reconstituted liposome system in which the P-glycoprotein has a uniformly inside-out orientation. bisbenzimide ethoxide trihydrochloride 56-69 ATP binding cassette subfamily B member 1 Homo sapiens 133-147 7608182-4 1995 We have used a fluorescent substrate of P-glycoprotein, Hoechst 33342, to measure transport activity in real-time of highly purified P-glycoprotein in a reconstituted liposome system in which the P-glycoprotein has a uniformly inside-out orientation. bisbenzimide ethoxide trihydrochloride 56-69 ATP binding cassette subfamily B member 1 Homo sapiens 133-147 7608182-9 1995 The Hoechst 33342 transport assay results are consistent with mechanisms in which P-glycoprotein alone is sufficient to transport drugs out of the membrane bilayer. bisbenzimide ethoxide trihydrochloride 4-17 ATP binding cassette subfamily B member 1 Homo sapiens 82-96 7632460-4 1995 Flow cytometric analysis using Hoechst 33342 and propidium iodide staining revealed that the Tat transfectants exhibited a higher percentage of apoptotic cells when compared to the control transfectants (29.1 +/- 3.1 vs. 11.43 +/- 3.1%). bisbenzimide ethoxide trihydrochloride 31-44 tyrosine aminotransferase Homo sapiens 93-96 7912637-1 1993 Starting from the observation of an efficient resistance to the vital dye Hoechst 33342, we postulated the presence of a P-glycoprotein in Dictyostelium discoideum cells. bisbenzimide ethoxide trihydrochloride 74-87 ATP binding cassette subfamily B member 1 Homo sapiens 121-135 7528122-7 1994 The IS1 cells stained as well with Hoechst 33342 as fixed 10B2 cells, and much better than unfixed 10B2 cells. bisbenzimide ethoxide trihydrochloride 35-48 IS1 Homo sapiens 4-7 8343560-5 1993 Simultaneous measurement of proliferation and determination of immunological subclass, as indicated with Hoechst 33342 staining and surface markers, showed an over-representation of CD19-positive cells, compared with CD2-positive cells and subsets of CD2-positive cells (CD4-positives and CD8-positives). bisbenzimide ethoxide trihydrochloride 105-118 CD19 molecule Homo sapiens 182-186 7912637-4 1993 Control experiments conducted without Hoechst 33342 demonstrated in these cells a constitutive expression of the P-glycoprotein, which was compared to its anthracycline-induced expression in a resistant human leukemic cell line, K562. bisbenzimide ethoxide trihydrochloride 38-51 ATP binding cassette subfamily B member 1 Homo sapiens 113-127 1401949-5 1992 Since Hoechst 33342 staining does not require destruction of the cell membrane, it is possible to directly phenotype cell surface antigen expression on Hoechst 33342bright lymphocytes by conventional immunofluorescence techniques and to evaluate membrane integrity of Hoechst 33342bright cells by dye exclusion criteria. bisbenzimide ethoxide trihydrochloride 6-19 CD53 molecule Homo sapiens 117-137 8440751-2 1993 In this report, we found that H342 completely abolished histone 2a mRNA but had no effect on alkaline phosphatase gene expression and protein synthesis in UMR 106-01 rat osteoblast-like cells. bisbenzimide ethoxide trihydrochloride 30-34 histone cluster 3, H2a Rattus norvegicus 56-66 8440751-3 1993 The complete loss of histone 2a mRNA occurred after only 20 min of treatment with H342. bisbenzimide ethoxide trihydrochloride 82-86 histone cluster 3, H2a Rattus norvegicus 21-31 1695009-5 1990 Up to 85% of the cells in a culture of human diploid dermal fibroblasts (HSF-55 cells) could be accumulated in G2 phase by placing cells presynchronized in early-S phase in medium containing Hoechst 33342 at 0.1 micrograms/ml for 10 hr. bisbenzimide ethoxide trihydrochloride 191-204 interleukin 6 Homo sapiens 73-76 1344288-7 1992 Dystrophin was found in the sarcolemma of myofibers containing FG-labeled nuclei but not of myofibers containing only Hoechst 33342-labeled nuclei. bisbenzimide ethoxide trihydrochloride 118-131 dystrophin, muscular dystrophy Mus musculus 0-10 1462732-8 1992 By staining with the vital dye Hoechst 33342 as well as with propidium iodide (PI) it was further possible to show that cell death after IL-3 withdrawal occurred in all phases of the cell cycle. bisbenzimide ethoxide trihydrochloride 31-44 interleukin 3 Mus musculus 137-141 2110526-5 1990 Using dual laser flow cytometry (uv: vital cell cycle with Hoechst 33342; 488 nm: DHFR with MTX-FITC), we show a maximum increase in the intracellular DHFR content during G1 and/or at G1/S transition (100 to 157%), followed by a continuous increase to 200% during S and G2/M. bisbenzimide ethoxide trihydrochloride 59-72 dihydrofolate reductase Cricetulus griseus 151-155 6332815-3 1984 In this study, the regulation of the rate of cell cycle progression of quiescent B6.1 cells after exposure to TCGF was analyzed using two complementary DNA staining techniques, namely, the propodium iodide method (to enumerate cells entering the S phase) and the Hoechst 33342-bromodeoxyuridine substitution technique (to enumerate cells which have gone through mitosis). bisbenzimide ethoxide trihydrochloride 263-276 interleukin 2 Mus musculus 110-114 34811747-5 2022 We noticed that besides H33342 being a specific marker for DNA, it also stains the transmembrane P-glycoprotein (P-gp) which is involved in the active pump-out of alien molecules from the cytoplasm; so, H33342 remains associated with P-gp after fixation. bisbenzimide ethoxide trihydrochloride 24-30 ATP binding cassette subfamily B member 1 Homo sapiens 97-111 34811747-5 2022 We noticed that besides H33342 being a specific marker for DNA, it also stains the transmembrane P-glycoprotein (P-gp) which is involved in the active pump-out of alien molecules from the cytoplasm; so, H33342 remains associated with P-gp after fixation. bisbenzimide ethoxide trihydrochloride 24-30 ATP binding cassette subfamily B member 1 Homo sapiens 113-117 34811747-5 2022 We noticed that besides H33342 being a specific marker for DNA, it also stains the transmembrane P-glycoprotein (P-gp) which is involved in the active pump-out of alien molecules from the cytoplasm; so, H33342 remains associated with P-gp after fixation. bisbenzimide ethoxide trihydrochloride 203-209 ATP binding cassette subfamily B member 1 Homo sapiens 97-111 34811747-5 2022 We noticed that besides H33342 being a specific marker for DNA, it also stains the transmembrane P-glycoprotein (P-gp) which is involved in the active pump-out of alien molecules from the cytoplasm; so, H33342 remains associated with P-gp after fixation. bisbenzimide ethoxide trihydrochloride 203-209 ATP binding cassette subfamily B member 1 Homo sapiens 113-117 34811747-5 2022 We noticed that besides H33342 being a specific marker for DNA, it also stains the transmembrane P-glycoprotein (P-gp) which is involved in the active pump-out of alien molecules from the cytoplasm; so, H33342 remains associated with P-gp after fixation. bisbenzimide ethoxide trihydrochloride 203-209 ATP binding cassette subfamily B member 1 Homo sapiens 234-238 34073890-3 2021 We employed a transwell-based in vitro cell model of iPSC-derived brain microvascular endothelial cells, where BCRP function was assessed by measuring the intracellular accumulation of its substrate Hoechst 33342. bisbenzimide ethoxide trihydrochloride 199-212 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 111-115 35567714-5 2022 In addition, the biological behaviors of H19X and miR-503-5p on CRC were examined in vitro and in vivo, including MTT, colony formation assay, Hoechst33342 and transwell assay. bisbenzimide ethoxide trihydrochloride 143-155 MIR503 host gene Homo sapiens 41-45 35477814-5 2022 We evaluated XPO1"s effects on cell proliferation and viability through WST-8 assays, cell cycle and apoptosis via Hoechst 33342 staining and flow cytometry, and intracellular signaling cascades using western blotting. bisbenzimide ethoxide trihydrochloride 115-128 exportin 1 Homo sapiens 13-17 3691678-3 1987 Accordingly, PtK1 cells were micronucleated with colchicine and their DNA was labeled by the Hoechst 33342 fluorochrome. bisbenzimide ethoxide trihydrochloride 93-106 mitogen-activated protein kinase kinase kinase 11 Homo sapiens 13-17 29596831-7 2018 Morphological changes caused by ginsenoside Rh4-induced apoptosis were also observed by Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 88-101 Rh blood group D antigen Homo sapiens 44-47 33576461-9 2021 The flow cytometry and Hoechst 33342/PI double staining results indicated that miR-150 overexpression significantly increased cell apoptosis compared with the mimic NC group. bisbenzimide ethoxide trihydrochloride 23-36 microRNA 150 Rattus norvegicus 79-86 6332978-3 1984 By using (a) bromodeoxyuridine quenching of Hoechst 33342 fluorescence and (b) the drug ICRF-159, a selective G2 - M-blocking agent, we show that dAdo causes a G1 block in cultured T leukemic cells and that cells in the S phase exposed to dAdo are able to complete that S phase, pass through G2 + M, and return to the G1 phase. bisbenzimide ethoxide trihydrochloride 44-57 ado Drosophila melanogaster 146-150 6153191-5 1980 Separation of these two populations from the spleen using the FACS and reanalyzing them for cell surface antigenic markers shows that the lymphocytes stained brightly with Hoechst 33342 are predominantly immunoglobulin positive, while the cells that stain less brightly express Thy 1.2. bisbenzimide ethoxide trihydrochloride 172-185 thymus cell antigen 1, theta Mus musculus 278-285 6085278-0 1984 Effects of the vital DNA probe fluorochrome Hoechst 33342 on PtK cell chromatin. bisbenzimide ethoxide trihydrochloride 44-57 protein tyrosine kinase 2 beta Homo sapiens 61-64 31931659-12 2020 Collectively, our results identify ROS as central inducers of MTORC2 activation during chronic autophagy, which in turn fuels senescence activation and myofibroblast differentiation in distinct cellular subpopulations.Abbreviations: 3-MA: 3-methyladenine; ACTA2: actin, alpha 2, smooth muscle, aorta; AKT1: AKT serine/threonine kinase 1; p-AKT1: AKT1 Ser473 phosphorylation; t-AKT1: total AKT serine/threonine kinase 1; ATG4A: autophagy related 4A cysteine peptidase; ATG7: autophagy gene 7; C12FDG: 5-dodecanoylaminofluorescein Di-beta-D-Galactopyranoside; CDKN1A: cyclin dependent kinase inhibitor 1A; CDKN2A: cyclin dependent kinase inhibitor 2A; Ctl: control; DAPI: 4",6-diamidino-2-phenylindole, dilactate; ECM: extracellular matrix; GSH: L-glutathione reduced; H2O2: hydrogen peroxide; HLF: adult human lung fibroblasts; Ho: Hoechst 33342 (2"-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2.5"-bi-1H-benzimidazole); HSC: hepatic stellate cells; LY: LY294002; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MTORC1/2: mechanistic target of rapamycin kinase complex 1/2; N: normal growth medium; NAC: N-acetyl-L-cysteine; PBS: phosphate-buffered saline; PDGFA: platelet derived growth factor subunit A; PRKCA/PKCalpha: protein kinase C alpha; PtdIns3K: class III phosphatidylinositol 3-kinase; PTEN: phosphatase and tensin homolog; R: rapamycin; RICTOR: RPTOR independent companion of MTOR complex 2; ROS: reactive oxygen species; RPTOR: regulatory associated protein of MTOR complex 1; SA-GLB1/beta-gal: senescence-associated galactosidase beta 1; SGK1: serum/glucocorticoid regulated kinase 1; shRNA: short hairpin RNA; siCtl: control siRNA; siRNA: small interfering RNA; SQSTM1: sequestosome 1; SS: serum-free (serum starvation) medium; TP53: tumor protein p53; TUBA: tubulin alpha; V: vehicle. bisbenzimide ethoxide trihydrochloride 846-917 mechanistic target of rapamycin kinase Homo sapiens 62-66 33307160-8 2021 Lacosamide revealed to inhibit BCRP in all tested concentrations (2.5-75 microM), exhibiting a significant increase (p<0.001) of the intracellular accumulation of a BCRP substrate (Hoechst 33342) in MDCK-BCRP cells. bisbenzimide ethoxide trihydrochloride 181-194 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 31-35 33307160-8 2021 Lacosamide revealed to inhibit BCRP in all tested concentrations (2.5-75 microM), exhibiting a significant increase (p<0.001) of the intracellular accumulation of a BCRP substrate (Hoechst 33342) in MDCK-BCRP cells. bisbenzimide ethoxide trihydrochloride 181-194 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 165-169 33199153-11 2021 Moreover, these novel N-phenyl-chromone-2-carboxamides inhibited ABCG2 in a Hoechst 33342 transport assay with potencies in the low three-digit nanomolar range, reversed MDR in cancer cells, were non-toxic and proved stable in blood plasma. bisbenzimide ethoxide trihydrochloride 76-89 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 65-70 31931659-12 2020 Collectively, our results identify ROS as central inducers of MTORC2 activation during chronic autophagy, which in turn fuels senescence activation and myofibroblast differentiation in distinct cellular subpopulations.Abbreviations: 3-MA: 3-methyladenine; ACTA2: actin, alpha 2, smooth muscle, aorta; AKT1: AKT serine/threonine kinase 1; p-AKT1: AKT1 Ser473 phosphorylation; t-AKT1: total AKT serine/threonine kinase 1; ATG4A: autophagy related 4A cysteine peptidase; ATG7: autophagy gene 7; C12FDG: 5-dodecanoylaminofluorescein Di-beta-D-Galactopyranoside; CDKN1A: cyclin dependent kinase inhibitor 1A; CDKN2A: cyclin dependent kinase inhibitor 2A; Ctl: control; DAPI: 4",6-diamidino-2-phenylindole, dilactate; ECM: extracellular matrix; GSH: L-glutathione reduced; H2O2: hydrogen peroxide; HLF: adult human lung fibroblasts; Ho: Hoechst 33342 (2"-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2.5"-bi-1H-benzimidazole); HSC: hepatic stellate cells; LY: LY294002; MAP1LC3B/LC3B: microtubule-associated protein 1 light chain 3 beta; MTORC1/2: mechanistic target of rapamycin kinase complex 1/2; N: normal growth medium; NAC: N-acetyl-L-cysteine; PBS: phosphate-buffered saline; PDGFA: platelet derived growth factor subunit A; PRKCA/PKCalpha: protein kinase C alpha; PtdIns3K: class III phosphatidylinositol 3-kinase; PTEN: phosphatase and tensin homolog; R: rapamycin; RICTOR: RPTOR independent companion of MTOR complex 2; ROS: reactive oxygen species; RPTOR: regulatory associated protein of MTOR complex 1; SA-GLB1/beta-gal: senescence-associated galactosidase beta 1; SGK1: serum/glucocorticoid regulated kinase 1; shRNA: short hairpin RNA; siCtl: control siRNA; siRNA: small interfering RNA; SQSTM1: sequestosome 1; SS: serum-free (serum starvation) medium; TP53: tumor protein p53; TUBA: tubulin alpha; V: vehicle. bisbenzimide ethoxide trihydrochloride 831-844 mechanistic target of rapamycin kinase Homo sapiens 62-66 31928387-10 2020 Consequently, efflux ability of cells increased and intracellular retention of doxorubicin and Hoechst 33342; substrates of ABCG2, decreased significantly.4. bisbenzimide ethoxide trihydrochloride 95-108 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 124-129 32787102-3 2020 The compounds competitively inhibited ABCG2-mediated Hoechst 33342 transport, but were not substrates of ABCG2. bisbenzimide ethoxide trihydrochloride 53-66 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 38-43 32323800-6 2020 ORP8 overexpression inhibited cell growth and induced apoptosis in NSCLC cells with MTS, anchorage-independent growth and Hoechst 33342 staining assay. bisbenzimide ethoxide trihydrochloride 122-135 oxysterol binding protein like 8 Homo sapiens 0-4 32205262-8 2020 Hoechst 33342, a specific substrate of ATP Binding Cassette Subfamily G Member 2 (ABCG2), was used to determine the effects of MTE on activities of ABCG2 in tumor cells. bisbenzimide ethoxide trihydrochloride 0-13 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 39-80 32205262-8 2020 Hoechst 33342, a specific substrate of ATP Binding Cassette Subfamily G Member 2 (ABCG2), was used to determine the effects of MTE on activities of ABCG2 in tumor cells. bisbenzimide ethoxide trihydrochloride 0-13 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 82-87 32205262-8 2020 Hoechst 33342, a specific substrate of ATP Binding Cassette Subfamily G Member 2 (ABCG2), was used to determine the effects of MTE on activities of ABCG2 in tumor cells. bisbenzimide ethoxide trihydrochloride 0-13 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 148-153 32239369-9 2020 Flow cytometric analysis and Hoechst 33342 staining exhibited potent apoptosis induction in U937 and A549 cells treated with NGR-sIL-24. bisbenzimide ethoxide trihydrochloride 29-42 reticulon 4 receptor Homo sapiens 125-128 31605952-7 2019 Using another NO-donor (DETNO), we show that NO directly inhibits the ATP activities of BCRP, inducing significant increases in the accumulations of both Hoechst 33342 dye and topotecan, substrates for BCRP. bisbenzimide ethoxide trihydrochloride 154-167 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 88-92 33005588-3 2020 The purpose of this study was, therefore, to examine the impacts of green tea catechins on BCRP activity in Caco-2 cells by H33342 (bis-benzamide, BCRP substrate) accumulation and AFB1 efflux. bisbenzimide ethoxide trihydrochloride 124-130 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 91-95 32105979-6 2020 Compounds UR-MB108 (57) and UR-MB136 (59) inhibited ABCG2 in a Hoechst 33342 transport assay with an IC50 value of about 80 nM and belong to the most potent ABCG2 inhibitors described so far. bisbenzimide ethoxide trihydrochloride 63-76 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 52-57 32105979-6 2020 Compounds UR-MB108 (57) and UR-MB136 (59) inhibited ABCG2 in a Hoechst 33342 transport assay with an IC50 value of about 80 nM and belong to the most potent ABCG2 inhibitors described so far. bisbenzimide ethoxide trihydrochloride 63-76 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 157-162 32138650-3 2020 These images are redundant in this figure as the images in parts D and E show Wnt3a treated and control cells stained with both Hoechst 33342 (as in parts A and B) and fluorescein diacetate. bisbenzimide ethoxide trihydrochloride 128-141 Wnt family member 3A Rattus norvegicus 78-83 31942786-5 2020 METHODS: We over-expressed AIRE or CARD domain of AIRE in GC1-spg cells and evaluated its impact on cell cycle using fluorescence activated cell sorting following Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 163-176 autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) Mus musculus 27-31 31942786-5 2020 METHODS: We over-expressed AIRE or CARD domain of AIRE in GC1-spg cells and evaluated its impact on cell cycle using fluorescence activated cell sorting following Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 163-176 autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) Mus musculus 50-54 32206278-2 2020 Confocal fluorescence microscopy studies on human fibroblast cells imaged after 18-24 h incubation show that Ir-CMYC concentrations of 80-100 muM promote good cell uptake and nuclear localisation, which was confirmed though co-localisation studies using Hoechst 33342. bisbenzimide ethoxide trihydrochloride 254-267 MYC proto-oncogene, bHLH transcription factor Homo sapiens 112-116 31605952-7 2019 Using another NO-donor (DETNO), we show that NO directly inhibits the ATP activities of BCRP, inducing significant increases in the accumulations of both Hoechst 33342 dye and topotecan, substrates for BCRP. bisbenzimide ethoxide trihydrochloride 154-167 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 202-206 31128021-7 2019 Hoechst 33342 staining and flow cytometry demonstrated that knockdown of ZEB2-AS1 obviously promoted the apoptosis of AGS cells. bisbenzimide ethoxide trihydrochloride 0-13 ZEB2 antisense RNA 1 Homo sapiens 73-81 30970221-3 2019 The mesenchymal stem cells (MSCs) were harvested from male rats and cytoprotective effect of this designer SAP (DSAP) on cultured MSCs was detected by Hoechst 33342 staining after being exposed to oxygen and glucose deprivation (OGD). bisbenzimide ethoxide trihydrochloride 151-164 amyloid P component, serum Rattus norvegicus 107-110 30540465-3 2019 Here, potential of mean force (PMF) calculations are used to identify the transport-competent minimum free energy binding locations of five compounds, Hoechst 33342, Rhodamine 123, paclitaxel, tariquidar, and verapamil to P-gp. bisbenzimide ethoxide trihydrochloride 151-164 phosphoglycolate phosphatase Homo sapiens 222-226 30628647-11 2019 Hoechst 33342 apoptosis staining indicated that apoptosis was increased in the L539fs/47-hERG-transfected cells, and this be reversed by treatment with 4-phenyl butyric acid. bisbenzimide ethoxide trihydrochloride 0-13 ETS transcription factor ERG Homo sapiens 89-93 30083516-5 2018 Results: Intrinsic susceptibility, oxygen-enhanced and dynamic contrast-enhanced MRI revealed significantly slower baseline R2* , lower hyperoxia-induced DeltaR2* and volume transfer constant Ktrans in the CALR tumors which were associated with significantly lower Hoechst 33342 uptake and greater pimonidazole-adduct formation. bisbenzimide ethoxide trihydrochloride 265-278 calreticulin Homo sapiens 206-210 30414939-9 2019 MCF-7 Tet-Off/ACSL4 cells showed greater doxorubicin, Hoechst 33342 and calcein AM efflux. bisbenzimide ethoxide trihydrochloride 54-67 acyl-CoA synthetase long chain family member 4 Homo sapiens 14-19 29683403-6 2018 The BCRP functionality was identified by flow cytometric analysis of mitoxantrone accumulation and fluorescence microscopic analysis of Hoechst 33342 accumulation using Ko-143 as BCRP inhibitor. bisbenzimide ethoxide trihydrochloride 136-149 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 4-8 29683403-9 2018 The verapamil induced a higher cellular uptake of Rhodamine 123, and Ko-143 significantly elevated cellular accumulation of mitoxantrone and Hoechst 33342, suggesting the P-gp and BCRP functionality shown by bEnd3 cells. bisbenzimide ethoxide trihydrochloride 141-154 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 180-184 29683403-9 2018 The verapamil induced a higher cellular uptake of Rhodamine 123, and Ko-143 significantly elevated cellular accumulation of mitoxantrone and Hoechst 33342, suggesting the P-gp and BCRP functionality shown by bEnd3 cells. bisbenzimide ethoxide trihydrochloride 141-154 BEN domain containing 3 Mus musculus 208-213 30187992-7 2019 TMF, HTMF and FTMF at 0.01-10 mum upregulated the K562/BCRP cellular accumulation of Hoechst 33342 nuclear staining dye. bisbenzimide ethoxide trihydrochloride 85-98 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 55-59 30075623-6 2018 The binding of substrate Hoechst 33342 and the two potent inhibitors 31 and 41 which differ in their mechanism of inhibition was rationalized using the recently published cryo-EM structures of ABCG2. bisbenzimide ethoxide trihydrochloride 25-38 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 193-198 29658564-8 2018 The results of the MTT, Hoechst 33342 and FCM assays demonstrated that overexpression of CtBP2 attenuated the reduction of cell viability and inhibited the cell apoptosis induced by DDP. bisbenzimide ethoxide trihydrochloride 24-37 C-terminal binding protein 2 Homo sapiens 89-94 29552127-14 2018 Hoechst 33342 nuclear staining confirmed that nNOS knock-down enhanced TMZ injury. bisbenzimide ethoxide trihydrochloride 0-13 nitric oxide synthase 1 Homo sapiens 46-50 29771418-7 2018 Hoechst 33342 staining and flow cytometry revealed that knocking down SNHG15 could significantly promote apoptosis of A549 cells. bisbenzimide ethoxide trihydrochloride 0-13 small nucleolar RNA host gene 15 Homo sapiens 70-76 31938416-6 2018 Hoechst 33342 fluorescence staining showed that a large number of autophagosomes were detected in miR-551b mimic group, fewer in the NC group, and only a small number in miR-551b inhibitor group. bisbenzimide ethoxide trihydrochloride 0-13 microRNA 551b Homo sapiens 98-106 28841513-10 2017 Additionally, the type of interaction between inhibitors and the ABCG2 substrate Hoechst 33342 was investigated yielding competitive and non-competitive interactions suggesting different modes of binding. bisbenzimide ethoxide trihydrochloride 81-94 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 65-70 29656289-11 2018 RESULTS: Hoechst 33342 staining revealed a one-tenth change in the percentage of Hoechst-positive cells after the addition of 500 ng/mL GDNF combined with 1,000 nM MTN for 24 h. The viability of the cells treated the same as described above was 1.4-fold that of the control group. bisbenzimide ethoxide trihydrochloride 9-22 glial cell derived neurotrophic factor Rattus norvegicus 136-140 29458405-14 2018 Dacomitinib was found to significantly increase the accumulation of ABCG2 probe substrates [doxorubicin (DOX),Rhodamine 123 (Rho 123) and Hoechst 33342] by inhibiting the transporter efflux function. bisbenzimide ethoxide trihydrochloride 138-151 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 68-73 29042181-7 2018 Then, to observe PGD2"s effects on cell count and apoptosis/mitosis (Hoechst 33342 stain), and migration (Transwell Assay), the cells were treated in vitro with physiological (<1muM) and/or supraphysiological (>1muM) concentrations of PGD2 over 72h. bisbenzimide ethoxide trihydrochloride 69-82 prostaglandin D2 synthase Homo sapiens 17-21 28949254-8 2017 Ko143 and YHO-13177 significantly inhibited the BCRP-mediated Hoechst 33342 transport in the 3D organoids. bisbenzimide ethoxide trihydrochloride 62-75 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 48-52 28471656-7 2017 Enzyme kinetic studies were carried out with Hoechst 33342 as fluorescent dye and substrate of ABCG2 to elucidate the compounds binding modes. bisbenzimide ethoxide trihydrochloride 45-58 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 95-100 28953230-5 2017 The activity of TR2-3 in inducing apoptosis in cancer cells was evaluated by using an Annexin V-PE apoptosis detection kit in combination with flow cytometry and the Hoechst 33342 and propidium iodide double staining analysis. bisbenzimide ethoxide trihydrochloride 166-179 nuclear receptor subfamily 2 group C member 1 Homo sapiens 16-21 28623970-9 2017 Treatment of BeWo cells with MbetaCD or pravastatin increased cellular retention of two BCRP substrates, the fluorescent dye Hoechst 33342 and the mycotoxin zearalenone. bisbenzimide ethoxide trihydrochloride 125-138 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 88-92 28245869-3 2017 The side population (SP) analysis has been used to detect the stem-like cancer cell populations based on their high expression of ABCG2 that exports Hoechst-33342 and certain cytotoxic drugs from the cells. bisbenzimide ethoxide trihydrochloride 149-162 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 130-135 27879033-5 2017 Rosiglitazone enhanced BCRP protein expression and transport activity, resulting in a 20% greater efflux of the substrate Hoechst 33342 compared with control cells. bisbenzimide ethoxide trihydrochloride 122-135 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 23-27 28401470-2 2017 H460 cells treated with the ethyl acetate extract of strain MCCB 248 and stained with Hoechst 33342 showed clear signs of apoptosis, including shrinkage of the cell nucleus, DNA fragmentation and chromatin condensation. bisbenzimide ethoxide trihydrochloride 86-99 methylcrotonyl-CoA carboxylase subunit 2 Homo sapiens 60-64 28380010-1 2017 The DNA intercalating dye Hoechst 33342 or its close analog DCV are actively removed from cells by the multidrug resistance transporter ABCG2, a protein overexpressed in metastatic cells and somatic stem cells. bisbenzimide ethoxide trihydrochloride 26-39 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 136-141 27431863-5 2016 PGP function was assessed with Hoechst 33342, and expression via western blotting. bisbenzimide ethoxide trihydrochloride 31-44 phosphoglycolate phosphatase Homo sapiens 0-3 28049953-5 2017 The BCRP inhibitor Ko143-sensitive accumulation of BCRP substrates such as Hoechst33342 and mitoxantrone was significantly enhanced by cobalt chloride treatment. bisbenzimide ethoxide trihydrochloride 75-87 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 4-8 28049953-5 2017 The BCRP inhibitor Ko143-sensitive accumulation of BCRP substrates such as Hoechst33342 and mitoxantrone was significantly enhanced by cobalt chloride treatment. bisbenzimide ethoxide trihydrochloride 75-87 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 51-55 28292469-6 2017 Reduced BCRP expression corresponded with impaired efflux activity during hypoxia as evidenced by accumulation of the substrate Hoechst 33342. bisbenzimide ethoxide trihydrochloride 128-141 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 8-12 27771282-4 2017 BCRP activity, when exposed to modulators for 1h, was diminished for most modulators through significant increases in H33342 accumulation at <10microm with 2,6,4-trimethoflavone increasing H33342 intracellular accumulation by 3.7-6.6 fold over 1-100microm. bisbenzimide ethoxide trihydrochloride 118-124 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 0-4 27771282-4 2017 BCRP activity, when exposed to modulators for 1h, was diminished for most modulators through significant increases in H33342 accumulation at <10microm with 2,6,4-trimethoflavone increasing H33342 intracellular accumulation by 3.7-6.6 fold over 1-100microm. bisbenzimide ethoxide trihydrochloride 192-198 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 0-4 27760172-6 2016 Although unrelated to the metastatic ability, SNAI2 inhibition does increase the efflux of Hoechst 33342 and enhance multidrug resistance in vitro and in vivo. bisbenzimide ethoxide trihydrochloride 91-104 snail family transcriptional repressor 2 Homo sapiens 46-51 27557646-2 2016 One approach, based on flow cytometry of murine testicular cells stained with Hoechst-33342 (Ho-FACS), has been extensively optimized and currently allows the isolation of 9 germ cell types. bisbenzimide ethoxide trihydrochloride 78-91 acyl-CoA synthetase long-chain family member 1 Mus musculus 96-100 26917208-3 2016 In MCF-7 cells, and in Hoechst 33342(lo) /CD44(hi) /CD24(lo) breast cancer stem-like cells isolated from MCF-7 cultures, ABCG2 was accumulated in cell-to-cell junction complexes and in large cytoplasmic aggresome-like vesicles. bisbenzimide ethoxide trihydrochloride 23-36 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 121-126 27417181-7 2016 TEER values increased in a time-dependent manner and reached approximately 100 Omega x cm(2) Efflux transport of Hoechst 33342, a substrate of breast cancer resistance protein (BCRP), was observed and inhibited by the BCRP inhibitor Ko143. bisbenzimide ethoxide trihydrochloride 113-126 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 143-175 27417181-7 2016 TEER values increased in a time-dependent manner and reached approximately 100 Omega x cm(2) Efflux transport of Hoechst 33342, a substrate of breast cancer resistance protein (BCRP), was observed and inhibited by the BCRP inhibitor Ko143. bisbenzimide ethoxide trihydrochloride 113-126 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 177-181 27417181-7 2016 TEER values increased in a time-dependent manner and reached approximately 100 Omega x cm(2) Efflux transport of Hoechst 33342, a substrate of breast cancer resistance protein (BCRP), was observed and inhibited by the BCRP inhibitor Ko143. bisbenzimide ethoxide trihydrochloride 113-126 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 218-222 26855420-5 2016 Moreover, overexpression of ALDH2 protected gastric mucosa cells against oxidative stress-induced apoptosis as determined by flow cytometry, Hoechst 33342, and TUNEL assays. bisbenzimide ethoxide trihydrochloride 141-154 aldehyde dehydrogenase 2 family member Homo sapiens 28-33 27172882-10 2016 The inhibitory effect of these macrocyclic lactones on the transport of two fluorophore probes, Rhodamine 123 and Hoechst 33342, by Dim-PGP-11 has been studied. bisbenzimide ethoxide trihydrochloride 114-127 phosphoglycolate phosphatase Homo sapiens 136-139 27172882-12 2016 However, both avermectins and milbemycin preventives inhibited the transport of Hoechst 33342 by Dim-PGP-11 in a concentration-dependent and apparently saturable manner, although differences existed in terms of efficiency and potency of inhibition between the two sub-classes of macrocyclic lactones. bisbenzimide ethoxide trihydrochloride 80-93 phosphoglycolate phosphatase Homo sapiens 101-104 27172882-13 2016 We postulate that Dim-PGP-11 may have two to three drug binding sites, as with mammalian Pgp, including the "R" site for Rhodamine 123 and the "H" site for Hoechst 33342. bisbenzimide ethoxide trihydrochloride 156-169 phosphoglycolate phosphatase Homo sapiens 22-25 27172882-13 2016 We postulate that Dim-PGP-11 may have two to three drug binding sites, as with mammalian Pgp, including the "R" site for Rhodamine 123 and the "H" site for Hoechst 33342. bisbenzimide ethoxide trihydrochloride 156-169 phosphoglycolate phosphatase Homo sapiens 89-92 27446443-7 2016 Nuclear condensation and fragmentation were also observed upon staining with Hoechst 33342 in FCP-treated A549 cells. bisbenzimide ethoxide trihydrochloride 77-90 FCP1 Homo sapiens 94-97 26055227-8 2016 Analysis with Hoechst 33342/PI double staining demonstrated that exposure to MK-801 (100 muM) for 24 h led to the death of 30 % of cultured cells (p < 0.05). bisbenzimide ethoxide trihydrochloride 14-27 latexin Homo sapiens 89-92 27602881-3 2016 Assessment of the transporter-dependent reduction of cellular uptake of the fluorescent dyes, such as Hoechst 33342 (Ho) and more recently DyeCycle Violet (DCV), have been widely advocated for the characterization of both ABCB1 and ABCG2 multidrug transporters. bisbenzimide ethoxide trihydrochloride 102-115 ATP binding cassette subfamily B member 1 Homo sapiens 222-227 27602881-3 2016 Assessment of the transporter-dependent reduction of cellular uptake of the fluorescent dyes, such as Hoechst 33342 (Ho) and more recently DyeCycle Violet (DCV), have been widely advocated for the characterization of both ABCB1 and ABCG2 multidrug transporters. bisbenzimide ethoxide trihydrochloride 102-115 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 232-237 27602881-3 2016 Assessment of the transporter-dependent reduction of cellular uptake of the fluorescent dyes, such as Hoechst 33342 (Ho) and more recently DyeCycle Violet (DCV), have been widely advocated for the characterization of both ABCB1 and ABCG2 multidrug transporters. bisbenzimide ethoxide trihydrochloride 102-104 ATP binding cassette subfamily B member 1 Homo sapiens 222-227 27602881-3 2016 Assessment of the transporter-dependent reduction of cellular uptake of the fluorescent dyes, such as Hoechst 33342 (Ho) and more recently DyeCycle Violet (DCV), have been widely advocated for the characterization of both ABCB1 and ABCG2 multidrug transporters. bisbenzimide ethoxide trihydrochloride 102-104 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 232-237 32263139-7 2016 Due to its low cytotoxicity, good membrane permeability and counterstain compatibility with the commercial fluorescent nucleic acid dye Hoechst 33342, as well as its ability to label RNA in living cells, OTP-ZnCl2 is a promising candidate for the detection of nucleic acid in living cells. bisbenzimide ethoxide trihydrochloride 136-149 orthopedia homeobox Homo sapiens 204-207 27222195-5 2016 Significantly increased cell apoptosis was observed in pReceiver-M29-DAPK-transfected HL-60 cells by flow cytometry and Hoechst33342 staining. bisbenzimide ethoxide trihydrochloride 120-132 death associated protein kinase 1 Homo sapiens 69-73 26639496-7 2015 MMR treated EAC cells showed membrane blebbing, chromatin condensation, nuclear fragmentation (apoptotic feature) in Hoechst 33342 staining under fluorescence microscope. bisbenzimide ethoxide trihydrochloride 117-130 ATPase, class II, type 9B Mus musculus 0-3 26706839-5 2016 The effective anti-adhesive concentration of ASF promotes p53 dependent apoptosis in MCF7, which was established by Hoechst 33342 staining, DNA fragmentation assay, FITC tagged Annexin-V flowcytometry and western blot analysis. bisbenzimide ethoxide trihydrochloride 116-129 serine and arginine rich splicing factor 1 Homo sapiens 45-48 26164758-8 2015 After transfection and apoptosis induction, Hoechst 33342 staining and PI flow cytometric assay showed that apoptosis was dramatically decreased in prostate cancer cells treated with miR-221/222 mimics. bisbenzimide ethoxide trihydrochloride 44-57 microRNA 221 Homo sapiens 183-190 26907376-10 2016 Human therapeutic plasma concentrations of all tested drugs caused a comparable competitive inhibition of H33342 excretion in both ABCG2 clones. bisbenzimide ethoxide trihydrochloride 106-112 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 131-136 26239082-3 2015 Here, we report a 3D in vitro model of spheroids with mixtures of cells expressing high and low levels of ABCG2, quantifying pump activity by the ability to reject the fluorescent dye Hoechst 33342. bisbenzimide ethoxide trihydrochloride 184-197 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 106-111 25833198-6 2015 These cells no longer excluded the BCRP substrate Hoechst 33342 and showed caspase activation and apoptosis induction. bisbenzimide ethoxide trihydrochloride 50-63 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 35-39 25976226-5 2015 BCRP inhibition was observed in Hoechst 33342 and pheophorbide A assays while P-gp inhibition was evaluated in calcein AM and rhodamine-123 assays. bisbenzimide ethoxide trihydrochloride 32-45 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 0-4 25953318-9 2015 Both PKI 166 and zAsp-DCB also inhibited the increase in number of apoptotic cells, as assessed by Hoechst 33342 staining and TUNEL assay. bisbenzimide ethoxide trihydrochloride 99-112 LIM domain binding 3a Danio rerio 17-21 26398332-3 2015 In the present study, it was found that MUC1 gene silencing not only resulted in the inhibition of SMMC-7721 cell growth, determined using a clone formation assay in vitro and a tumor xenograft mouse model with an in vivo imaging system, but also induced apoptotic alterations in SMMC-7721 cells, determined using Hoechst 33342 staining, flow cytometry with an Annexin V-PE staining and a DNA ladder assay. bisbenzimide ethoxide trihydrochloride 314-327 mucin 1, cell surface associated Homo sapiens 40-44 26247574-2 2015 METHODS: miR-345 was overexpressed in PC cells by stable transfection, and its effect on growth, apoptosis and mitochondrial-membrane potential was examined by WST-1, Hoechst-33342/Annexin-V, and JC-1 staining, respectively. bisbenzimide ethoxide trihydrochloride 167-180 microRNA 345 Homo sapiens 9-16 25955519-4 2015 We found that following exposure to OGD or H2O2, the knockdown of Srxn1 resulted in a decrease in cell viability, as shown by MTS assay, an increase in cell damage, as shown by lactate dehydrogenase assay and an increase in cellular apoptosis, as shown by Hoechst 33342 staining and flow cytometry. bisbenzimide ethoxide trihydrochloride 256-269 sulfiredoxin 1 Rattus norvegicus 66-71 26035796-5 2015 Treatment with ADM significantly blunted hypoxic-induced apoptosis, evaluated by Hoechst 33342 staining and Annexin V-FITC/PI labeling. bisbenzimide ethoxide trihydrochloride 81-94 adrenomedullin Homo sapiens 15-18 25835358-2 2015 Their inhibitory activity on the drug efflux of breast cancer resistance protein (ABCG2) was evaluated by flow cytometric analysis of accumulation of Hoechst 33342 stain in Flp-In-293/ABCG2 cells. bisbenzimide ethoxide trihydrochloride 150-163 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 48-80 25865432-5 2015 Avermectins inhibited MDR1/Mdr1a-mediated H33342 dye efflux, with apparent Ki values of 0.24+-0.08 and 0.18+-0.02muM (ivermectin); 0.60+-0.07 and 0.56+-0.02muM (emamectin) and 0.95+-0.08 and 0.77+-0.25muM (abamectin) in SH-SY5Y and N2a cells, respectively. bisbenzimide ethoxide trihydrochloride 42-48 ATP binding cassette subfamily B member 1 Homo sapiens 22-26 25865432-5 2015 Avermectins inhibited MDR1/Mdr1a-mediated H33342 dye efflux, with apparent Ki values of 0.24+-0.08 and 0.18+-0.02muM (ivermectin); 0.60+-0.07 and 0.56+-0.02muM (emamectin) and 0.95+-0.08 and 0.77+-0.25muM (abamectin) in SH-SY5Y and N2a cells, respectively. bisbenzimide ethoxide trihydrochloride 42-48 ATP-binding cassette, sub-family B (MDR/TAP), member 1A Mus musculus 27-32 25418064-8 2015 Functional ABCB1/G2 activity was demonstrated by exclusion of the substrate dye, Hoechst 33342. bisbenzimide ethoxide trihydrochloride 81-94 ATP binding cassette subfamily B member 1 Homo sapiens 11-16 26269753-6 2015 Furthermore, serum deprivation-induced apoptotic death, assessed by Hoechst 33342 staining and fluorescent microscopy, increased significantly in the Wnt10B-silencing cells. bisbenzimide ethoxide trihydrochloride 68-81 Wnt family member 10B Homo sapiens 150-156 25835358-2 2015 Their inhibitory activity on the drug efflux of breast cancer resistance protein (ABCG2) was evaluated by flow cytometric analysis of accumulation of Hoechst 33342 stain in Flp-In-293/ABCG2 cells. bisbenzimide ethoxide trihydrochloride 150-163 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 82-87 25835358-2 2015 Their inhibitory activity on the drug efflux of breast cancer resistance protein (ABCG2) was evaluated by flow cytometric analysis of accumulation of Hoechst 33342 stain in Flp-In-293/ABCG2 cells. bisbenzimide ethoxide trihydrochloride 150-163 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 184-189 26052432-7 2015 Further, genistein, zearalenone, and tributyltin increased the retention of the fluorescent BCRP substrate, Hoechst 33342, between 50-100% in BeWo cells. bisbenzimide ethoxide trihydrochloride 108-121 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 92-96 25827485-1 2015 Protocols for purification of murine male germ cells by FACS based on Hoechst 33342 (Ho342) dye staining have been reported and optimized. bisbenzimide ethoxide trihydrochloride 70-83 acyl-CoA synthetase long-chain family member 1 Mus musculus 56-60 25583725-8 2015 Increased expression of AcrAB-OprM pump genes after cold shock leads to a lower accumulation of Hoechst 33342 (H33342), a substrate of AcrAB-OprM efflux pumps, indicating that cold shock results in increased efflux activity. bisbenzimide ethoxide trihydrochloride 96-109 opioid receptor mu 1 Homo sapiens 30-34 25583725-8 2015 Increased expression of AcrAB-OprM pump genes after cold shock leads to a lower accumulation of Hoechst 33342 (H33342), a substrate of AcrAB-OprM efflux pumps, indicating that cold shock results in increased efflux activity. bisbenzimide ethoxide trihydrochloride 96-109 opioid receptor mu 1 Homo sapiens 141-145 25960227-5 2015 Alternatively, caspases inhibitors could lead to the disappearance of MHMD-eliciting nuclei fragmentation by Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 109-122 caspase 8 Homo sapiens 15-23 25827485-1 2015 Protocols for purification of murine male germ cells by FACS based on Hoechst 33342 (Ho342) dye staining have been reported and optimized. bisbenzimide ethoxide trihydrochloride 85-90 acyl-CoA synthetase long-chain family member 1 Mus musculus 56-60 25824548-6 2015 Furthermore, flow cytometric analysis and Hoechst33342 staining demonstrated that let-7g increased the apoptotic rate of cultured granulosa cells. bisbenzimide ethoxide trihydrochloride 42-54 microRNA let-7g Homo sapiens 82-88 25661164-10 2015 In cultured murine podocytes, ADR stimulated the expression of Bax/Bcl-2 and apoptosis as determined by Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 104-117 BCL2-associated X protein Mus musculus 63-66 24846829-4 2015 In this study, 18 h of hypoxia increased hypoxia inducible factor 1 alpha (Hif1alpha) mRNA expression and induced apoptotic processes, such as loss of the mitochondrial membrane potential, activation of caspase-3 and fragmentation of cell nuclei based on Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 255-268 hypoxia inducible factor 1, alpha subunit Mus musculus 41-73 24846829-4 2015 In this study, 18 h of hypoxia increased hypoxia inducible factor 1 alpha (Hif1alpha) mRNA expression and induced apoptotic processes, such as loss of the mitochondrial membrane potential, activation of caspase-3 and fragmentation of cell nuclei based on Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 255-268 hypoxia inducible factor 1, alpha subunit Mus musculus 75-84 25013968-11 2014 This work provides a physiological mechanism that unites the DBL-1 signaling pathway roles of not only body size regulation and drug responsiveness, but also the novel Hoechst 33342 staining and aggregation phenotypes, through barrier function, content, and organization of the cuticle. bisbenzimide ethoxide trihydrochloride 168-181 Protein dbl-1 Caenorhabditis elegans 61-66 25426258-5 2014 Different methods for their identification and isolation through stemness markers using various in vivo and in vitro methods such as flow cytometry, thyrosphere formation assay, aldehyde dehydrogenase activity and ATP-binding cassette sub-family G member 2 efflux-pump mediated Hoechst 33342 dye exclusion have been discussed. bisbenzimide ethoxide trihydrochloride 278-291 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 214-256 24280300-3 2014 The suspension mouse lymphocyte cancer cells (L5178Y cells) labeled by Hoechst33342 freely flowed into the surface of the SPR sensor chip. bisbenzimide ethoxide trihydrochloride 71-83 sepiapterin reductase Mus musculus 122-125 24676941-4 2014 In the present study, lentiviral vector-mediated ART1-cDNA was transfected into CT26 cells, and the apoptosis rate was detected by flow cytometric assay and Hoechst 33342 staining. bisbenzimide ethoxide trihydrochloride 157-170 ADP-ribosyltransferase 1 Mus musculus 49-53 24497321-7 2014 This characteristic allows for SP cells to be isolated based upon their capacity to efflux the dye Hoechst 33342, through a mechanism driven by a membrane transporter, the breast cancer resistance protein (BCRP1/ABCG2). bisbenzimide ethoxide trihydrochloride 99-112 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 206-211 24497321-7 2014 This characteristic allows for SP cells to be isolated based upon their capacity to efflux the dye Hoechst 33342, through a mechanism driven by a membrane transporter, the breast cancer resistance protein (BCRP1/ABCG2). bisbenzimide ethoxide trihydrochloride 99-112 ATP binding cassette subfamily G member 2 (Junior blood group) Mus musculus 212-217 24219411-7 2014 In contrast, QZ59-RRR non-competitively inhibited daunorubicin transport with moderate efficacy (K(I,app) = 1.9 muM); it also displayed a mixed-type inhibition of the Hoechst 33342 transport, resulting from a main non-competitive tendency (K(i2,app) = 1.6 muM) and a limited competitive tendency (K(i1,app) = 5 muM). bisbenzimide ethoxide trihydrochloride 167-180 latexin Homo sapiens 256-259 24219411-7 2014 In contrast, QZ59-RRR non-competitively inhibited daunorubicin transport with moderate efficacy (K(I,app) = 1.9 muM); it also displayed a mixed-type inhibition of the Hoechst 33342 transport, resulting from a main non-competitive tendency (K(i2,app) = 1.6 muM) and a limited competitive tendency (K(i1,app) = 5 muM). bisbenzimide ethoxide trihydrochloride 167-180 latexin Homo sapiens 256-259 24369154-6 2013 The cells transfected with CaMKIIN were stained with Hoechst 33342 to detect the apoptotic proportion under fluorescence microscopy. bisbenzimide ethoxide trihydrochloride 53-66 calcium/calmodulin dependent protein kinase II inhibitor 2 Homo sapiens 27-34 24376706-3 2013 Four of the compounds inhibited efflux of two ABCB1 substrates, Hoechst 33342 and daunorubicin, in MDCKII-ABCB1 cells: Olomoucine II most strongly, followed by roscovitine, purvalanol A, and flavopiridol. bisbenzimide ethoxide trihydrochloride 64-77 ATP binding cassette subfamily B member 1 Homo sapiens 46-51 24376706-3 2013 Four of the compounds inhibited efflux of two ABCB1 substrates, Hoechst 33342 and daunorubicin, in MDCKII-ABCB1 cells: Olomoucine II most strongly, followed by roscovitine, purvalanol A, and flavopiridol. bisbenzimide ethoxide trihydrochloride 64-77 ATP binding cassette subfamily B member 1 Homo sapiens 106-111 24376706-4 2013 SNS-032 inhibited ABCB1-mediated efflux of Hoechst 33342 but not daunorubicin. bisbenzimide ethoxide trihydrochloride 43-56 ATP binding cassette subfamily B member 1 Homo sapiens 18-23 23835215-6 2013 In especial, CAE possessed well membrane-permeability, brilliant selectivity among various bioanalyte and excellent counterstain compatibility with Hoechst 33342, MTR and LTR. bisbenzimide ethoxide trihydrochloride 148-161 gap junction protein alpha 8 Homo sapiens 13-16 23851114-5 2013 All synthesized compounds were tested for BCRP inhibition in Hoechst 33342 and pheophorbide A accumulation assays using MDCK cells expressing BCRP. bisbenzimide ethoxide trihydrochloride 61-74 ATP binding cassette subfamily G member 2 Canis lupus familiaris 42-46 24002436-7 2013 All four factors influenced SP percentage, when the other three conditions were fixed, the optimal Hoechst 33342 concentrations determined were 11 microg/ml for PLC/PRF/5 cells, 4 microg/ml for Huh-7 and 5 microg/ml for Hep-3B cells. bisbenzimide ethoxide trihydrochloride 99-112 heparan sulfate proteoglycan 2 Homo sapiens 161-164 24002436-7 2013 All four factors influenced SP percentage, when the other three conditions were fixed, the optimal Hoechst 33342 concentrations determined were 11 microg/ml for PLC/PRF/5 cells, 4 microg/ml for Huh-7 and 5 microg/ml for Hep-3B cells. bisbenzimide ethoxide trihydrochloride 99-112 MIR7-3 host gene Homo sapiens 194-205 23881407-7 2013 Furthermore, the remarkable low-efflux ability of Hoechst 33342 from TtT/GF cells was confirmed by using inhibitors and contrasted with the abilities of Tpit/F1 and LbetaT2 cells. bisbenzimide ethoxide trihydrochloride 50-63 T-box 19 Mus musculus 153-157 23907512-8 2013 Thiazolyl blue tetrazolium bromide assay, Hoechst33342/Propidium Iodide staining and Western blot assay showed that Tat-CRMP2 pretreatment increased cell viability compared with the control group against NMDA exposure by decreasing the cleavage of CRMP2. bisbenzimide ethoxide trihydrochloride 42-54 tyrosine aminotransferase Homo sapiens 116-119 23907512-8 2013 Thiazolyl blue tetrazolium bromide assay, Hoechst33342/Propidium Iodide staining and Western blot assay showed that Tat-CRMP2 pretreatment increased cell viability compared with the control group against NMDA exposure by decreasing the cleavage of CRMP2. bisbenzimide ethoxide trihydrochloride 42-54 dihydropyrimidinase like 2 Homo sapiens 120-125