PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 18776693-4 2008 Moreover, additional inhibitory effects of thiamine pyrophosphate (TPP) and hemin on folate uptake after PI-PLC treatment suggested that not only FRalpha but also RFC and heme carrier protein 1 (HCP1) are involved in the folate transport mechanism in the human placenta. Thiamine Pyrophosphate 43-65 phospholipase C beta 1 Homo sapiens 105-111 18776693-4 2008 Moreover, additional inhibitory effects of thiamine pyrophosphate (TPP) and hemin on folate uptake after PI-PLC treatment suggested that not only FRalpha but also RFC and heme carrier protein 1 (HCP1) are involved in the folate transport mechanism in the human placenta. Thiamine Pyrophosphate 43-65 FOS like 1, AP-1 transcription factor subunit Homo sapiens 146-153 18776693-4 2008 Moreover, additional inhibitory effects of thiamine pyrophosphate (TPP) and hemin on folate uptake after PI-PLC treatment suggested that not only FRalpha but also RFC and heme carrier protein 1 (HCP1) are involved in the folate transport mechanism in the human placenta. Thiamine Pyrophosphate 43-65 solute carrier family 46 member 1 Homo sapiens 195-199 18776693-4 2008 Moreover, additional inhibitory effects of thiamine pyrophosphate (TPP) and hemin on folate uptake after PI-PLC treatment suggested that not only FRalpha but also RFC and heme carrier protein 1 (HCP1) are involved in the folate transport mechanism in the human placenta. Thiamine Pyrophosphate 67-70 phospholipase C beta 1 Homo sapiens 105-111 18776693-4 2008 Moreover, additional inhibitory effects of thiamine pyrophosphate (TPP) and hemin on folate uptake after PI-PLC treatment suggested that not only FRalpha but also RFC and heme carrier protein 1 (HCP1) are involved in the folate transport mechanism in the human placenta. Thiamine Pyrophosphate 67-70 FOS like 1, AP-1 transcription factor subunit Homo sapiens 146-153 18570438-9 2008 Both spectroscopic and X-ray structural studies are consistent with inhibition resulting from the binding of MBP to the thiamin diphosphate in the active centers. Thiamine Pyrophosphate 120-139 myelin basic protein Homo sapiens 109-112 18533652-2 2008 In plants, TPP regulates its own production by binding to the 3" untranslated region of the mRNA encoding ThiC, a critical enzyme in thiamine biosynthesis, which promotes the formation of an unstable splicing variant. Thiamine Pyrophosphate 11-14 thiaminC Arabidopsis thaliana 106-110 17956236-1 2007 2-Hydroxyphytanoyl-CoA lyase (abbreviated as 2-HPCL), renamed to 2-hydroxyacyl-CoA lyase (abbreviated as HACL1), is the first peroxisomal enzyme in mammals that has been found to be dependent on TPP (thiamin pyrophosphate). Thiamine Pyrophosphate 195-198 2-hydroxyacyl-CoA lyase 1 Homo sapiens 45-51 18280798-1 2008 We review the evidence that the function of the SLC25A19 gene product, previously identified as the mitochondrial deoxyribonucleotide carrier (DNC), is actually the transport of thiamine pyrophosphate. Thiamine Pyrophosphate 178-200 solute carrier family 25 member 19 Homo sapiens 48-56 18280798-1 2008 We review the evidence that the function of the SLC25A19 gene product, previously identified as the mitochondrial deoxyribonucleotide carrier (DNC), is actually the transport of thiamine pyrophosphate. Thiamine Pyrophosphate 178-200 solute carrier family 25 member 19 Homo sapiens 143-146 18276586-1 2008 Mammalian soluble thiamine triphosphatase (ThTPase) is a 25-kDa cytosolic enzyme that specifically catalyzes the conversion of thiamine triphosphate (ThTP) to thiamine diphosphate and has an absolute requirement for divalent cations. Thiamine Pyrophosphate 159-179 thiamine triphosphatase Homo sapiens 18-41 18276586-1 2008 Mammalian soluble thiamine triphosphatase (ThTPase) is a 25-kDa cytosolic enzyme that specifically catalyzes the conversion of thiamine triphosphate (ThTP) to thiamine diphosphate and has an absolute requirement for divalent cations. Thiamine Pyrophosphate 159-179 thiamine triphosphatase Homo sapiens 43-50 17956236-1 2007 2-Hydroxyphytanoyl-CoA lyase (abbreviated as 2-HPCL), renamed to 2-hydroxyacyl-CoA lyase (abbreviated as HACL1), is the first peroxisomal enzyme in mammals that has been found to be dependent on TPP (thiamin pyrophosphate). Thiamine Pyrophosphate 195-198 2-hydroxyacyl-CoA lyase 1 Homo sapiens 105-110 17956236-1 2007 2-Hydroxyphytanoyl-CoA lyase (abbreviated as 2-HPCL), renamed to 2-hydroxyacyl-CoA lyase (abbreviated as HACL1), is the first peroxisomal enzyme in mammals that has been found to be dependent on TPP (thiamin pyrophosphate). Thiamine Pyrophosphate 200-221 2-hydroxyacyl-CoA lyase 1 Homo sapiens 45-51 17956236-1 2007 2-Hydroxyphytanoyl-CoA lyase (abbreviated as 2-HPCL), renamed to 2-hydroxyacyl-CoA lyase (abbreviated as HACL1), is the first peroxisomal enzyme in mammals that has been found to be dependent on TPP (thiamin pyrophosphate). Thiamine Pyrophosphate 200-221 2-hydroxyacyl-CoA lyase 1 Homo sapiens 105-110 17914811-0 2007 Reactions of fac-[Re(CO)3(H2O)3]+ with nucleoside diphosphates and thiamine diphosphate in aqueous solution investigated by multinuclear NMR spectroscopy. Thiamine Pyrophosphate 67-87 FA complementation group C Homo sapiens 13-16 17914867-1 2007 Transketolase is a prominent thiamin diphosphate-dependent enzyme in sugar metabolism that catalyzes the reversible transfer of a 2-carbon dihydroxyethyl fragment between a donor ketose and an acceptor aldose. Thiamine Pyrophosphate 29-48 transketolase Homo sapiens 0-13 17914811-3 2007 Thiamine diphosphate (TDP) and uridine 5"-diphosphate (5"-UDP) form 1:1 bidentate {Palpha,Pbeta} chelates, in which the MDP binds Re(I) via Palpha and Pbeta phosphate groups. Thiamine Pyrophosphate 0-20 dipeptidase 1 Homo sapiens 120-123 17596263-1 2007 BACKGROUND: Thiamine pyrophosphate (TPP) is a cofactor for 2-hydroxyacyl-CoA lyase 1 (HACL1), a peroxisomal enzyme essential for the alpha-oxidation of phytanic acid and 2-hydroxy straight chain fatty acids. Thiamine Pyrophosphate 12-34 2-hydroxyacyl-CoA lyase 1 Homo sapiens 86-91 17596263-1 2007 BACKGROUND: Thiamine pyrophosphate (TPP) is a cofactor for 2-hydroxyacyl-CoA lyase 1 (HACL1), a peroxisomal enzyme essential for the alpha-oxidation of phytanic acid and 2-hydroxy straight chain fatty acids. Thiamine Pyrophosphate 36-39 2-hydroxyacyl-CoA lyase 1 Homo sapiens 86-91 17596263-2 2007 So far, HACL1 is the only known peroxisomal TPP-dependent enzyme in mammals. Thiamine Pyrophosphate 44-47 2-hydroxyacyl-CoA lyase 1 Homo sapiens 8-13 17596263-10 2007 Consequently, TPP entry may depend on a specific transport system or, in a bound form, on HACL1 translocation. Thiamine Pyrophosphate 14-17 2-hydroxyacyl-CoA lyase 1 Homo sapiens 90-95 17454302-6 2007 2004, 271, 4189 - 4194) we reported that the affinity of ThDP for TK considerably increases in the presence of the donor substrate, which may be a mechanism whereby the activity of the enzyme is regulated under the conditions of the coenzyme deficiency. Thiamine Pyrophosphate 57-61 transketolase Homo sapiens 66-68 17329260-2 2007 We address this question by investigating the ThDP-dependent decarboxylase/dehydrogenase (E1b) component of the mitochondrial branched-chain alpha-keto acid dehydrogenase complex (BCKDC). Thiamine Pyrophosphate 46-50 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 90-93 17329260-9 2007 The above results provide evidence that the two active sites in the E1b heterotetramer operate independently during the ThDP-dependent decarboxylation reaction. Thiamine Pyrophosphate 120-124 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 68-71 17309441-0 2007 Influence of donor substrate on kinetic parameters of thiamine diphosphate binding to transketolase. Thiamine Pyrophosphate 54-74 transketolase Homo sapiens 86-99 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 41-61 transketolase Homo sapiens 74-87 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 41-61 transketolase Homo sapiens 89-91 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 41-61 transketolase Homo sapiens 111-113 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 41-61 transketolase Homo sapiens 111-113 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 41-61 transketolase Homo sapiens 111-113 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 63-67 transketolase Homo sapiens 74-87 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 63-67 transketolase Homo sapiens 89-91 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 63-67 transketolase Homo sapiens 111-113 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 63-67 transketolase Homo sapiens 111-113 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 63-67 transketolase Homo sapiens 111-113 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 116-120 transketolase Homo sapiens 74-87 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 116-120 transketolase Homo sapiens 89-91 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 116-120 transketolase Homo sapiens 74-87 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 116-120 transketolase Homo sapiens 89-91 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 116-120 transketolase Homo sapiens 74-87 17309441-1 2007 The two-step mechanism of interaction of thiamine diphosphate (ThDP) with transketolase (TK) has been studied: TK + ThDP <--> TK...ThDP <--> TK*-ThDP. Thiamine Pyrophosphate 116-120 transketolase Homo sapiens 89-91 17309441-2 2007 The scheme involves the formation of inactive intermediate complex TK...ThDP followed by its transformation into catalytically active holoenzyme, TK*-ThDP. Thiamine Pyrophosphate 72-76 transketolase Homo sapiens 67-69 17309441-2 2007 The scheme involves the formation of inactive intermediate complex TK...ThDP followed by its transformation into catalytically active holoenzyme, TK*-ThDP. Thiamine Pyrophosphate 72-76 transketolase Homo sapiens 146-148 17309441-2 2007 The scheme involves the formation of inactive intermediate complex TK...ThDP followed by its transformation into catalytically active holoenzyme, TK*-ThDP. Thiamine Pyrophosphate 150-154 transketolase Homo sapiens 67-69 17309441-2 2007 The scheme involves the formation of inactive intermediate complex TK...ThDP followed by its transformation into catalytically active holoenzyme, TK*-ThDP. Thiamine Pyrophosphate 150-154 transketolase Homo sapiens 146-148 16850348-2 2006 In this work, we addressed the role of Pdc2 in the coordinated control of biosynthesis and demand of an essential metabolic cofactor, thiaminediphosphate (ThDP). Thiamine Pyrophosphate 134-153 Pdc2p Saccharomyces cerevisiae S288C 39-43 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 19-39 transketolase Homo sapiens 52-65 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 19-39 transketolase Homo sapiens 67-69 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 19-39 transketolase Homo sapiens 179-181 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 19-39 transketolase Homo sapiens 179-181 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 41-45 transketolase Homo sapiens 52-65 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 41-45 transketolase Homo sapiens 67-69 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 41-45 transketolase Homo sapiens 179-181 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 41-45 transketolase Homo sapiens 179-181 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 184-188 transketolase Homo sapiens 52-65 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 184-188 transketolase Homo sapiens 67-69 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 184-188 transketolase Homo sapiens 179-181 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 184-188 transketolase Homo sapiens 179-181 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 184-188 transketolase Homo sapiens 52-65 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 184-188 transketolase Homo sapiens 67-69 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 184-188 transketolase Homo sapiens 179-181 17454302-1 2007 The interaction of thiamine diphosphate (ThDP) with transketolase (TK) involves at least two stages: [formula: see text] During the first stage, an inactive intermediate complex (TK...ThDP) is formed, which is then transformed into a catalytically active holoenzyme (TK* - ThDP). Thiamine Pyrophosphate 184-188 transketolase Homo sapiens 179-181 17035501-0 2006 Knockout of Slc25a19 causes mitochondrial thiamine pyrophosphate depletion, embryonic lethality, CNS malformations, and anemia. Thiamine Pyrophosphate 42-64 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 Mus musculus 12-20 17035501-7 2006 We identified thiamine pyrophosphate (ThPP) transport as a candidate function of SLC25A19 through homology searching and confirmed it by using transport assays of the recombinant reconstituted protein. Thiamine Pyrophosphate 14-36 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 Mus musculus 81-89 17035501-7 2006 We identified thiamine pyrophosphate (ThPP) transport as a candidate function of SLC25A19 through homology searching and confirmed it by using transport assays of the recombinant reconstituted protein. Thiamine Pyrophosphate 38-42 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 Mus musculus 81-89 17035501-8 2006 The mitochondria of Slc25a19(-/-) and MCPHA cells have undetectable and markedly reduced ThPP content, respectively. Thiamine Pyrophosphate 89-93 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 Mus musculus 20-28 16850348-2 2006 In this work, we addressed the role of Pdc2 in the coordinated control of biosynthesis and demand of an essential metabolic cofactor, thiaminediphosphate (ThDP). Thiamine Pyrophosphate 155-159 Pdc2p Saccharomyces cerevisiae S288C 39-43 16850348-4 2006 The Pdc2 has also been implicated as a regulator of genes encoding enzymes in ThDP metabolism. Thiamine Pyrophosphate 78-82 Pdc2p Saccharomyces cerevisiae S288C 4-8 16850348-9 2006 While the Thi2, in conjunction with Thi3, seems to control expression of THI genes with respect to thiamine availability, the Pdc2 may link the ThDP demand to carbon source availability. Thiamine Pyrophosphate 144-148 Pdc2p Saccharomyces cerevisiae S288C 126-130 16786225-1 2006 The identification of 2-hydroxyphytanoyl-CoA lyase (2-HPCL), a thiamine pyrophosphate (TPP)-dependent peroxisomal enzyme involved in the alpha-oxidation of phytanic acid and of 2-hydroxy straight chain fatty acids, pointed towards a role of TPP in these processes. Thiamine Pyrophosphate 63-85 2-hydroxyacyl-CoA lyase 1 Homo sapiens 22-50 16786225-1 2006 The identification of 2-hydroxyphytanoyl-CoA lyase (2-HPCL), a thiamine pyrophosphate (TPP)-dependent peroxisomal enzyme involved in the alpha-oxidation of phytanic acid and of 2-hydroxy straight chain fatty acids, pointed towards a role of TPP in these processes. Thiamine Pyrophosphate 63-85 2-hydroxyacyl-CoA lyase 1 Homo sapiens 52-58 16786225-1 2006 The identification of 2-hydroxyphytanoyl-CoA lyase (2-HPCL), a thiamine pyrophosphate (TPP)-dependent peroxisomal enzyme involved in the alpha-oxidation of phytanic acid and of 2-hydroxy straight chain fatty acids, pointed towards a role of TPP in these processes. Thiamine Pyrophosphate 87-90 2-hydroxyacyl-CoA lyase 1 Homo sapiens 22-50 16786225-1 2006 The identification of 2-hydroxyphytanoyl-CoA lyase (2-HPCL), a thiamine pyrophosphate (TPP)-dependent peroxisomal enzyme involved in the alpha-oxidation of phytanic acid and of 2-hydroxy straight chain fatty acids, pointed towards a role of TPP in these processes. Thiamine Pyrophosphate 87-90 2-hydroxyacyl-CoA lyase 1 Homo sapiens 52-58 16563643-6 2006 Thiamine (6 mM), and dihydrolipoic acid (50 microM), required cofactors for pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase (thiamine as thiamine pyrophosphate), attenuated the reactive oxygen species-induced reductions in these enzyme activities, as well as subsequent loss of mitochondrial transmembrane potential and ATP, and neuronal death. Thiamine Pyrophosphate 150-172 oxoglutarate dehydrogenase Rattus norvegicus 103-136 16194233-2 2005 In this yeast THI regulatory system acts mainly at the transcriptional level, thiamin pyrophosphate (TDP) seems to serve as a corepressor, and genetic studies have identified three positive regulatory factors (Thi2p, Thi3p and Pdc2p). Thiamine Pyrophosphate 78-99 Pdc2p Saccharomyces cerevisiae S288C 227-232 16472748-1 2006 The dehydrogenase/decarboxylase (E1b) component of the 4 MD human branched-chain alpha-ketoacid dehydrogenase complex (BCKDC) is a thiamin diphosphate (ThDP)-dependent enzyme. Thiamine Pyrophosphate 131-150 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 33-36 16472748-1 2006 The dehydrogenase/decarboxylase (E1b) component of the 4 MD human branched-chain alpha-ketoacid dehydrogenase complex (BCKDC) is a thiamin diphosphate (ThDP)-dependent enzyme. Thiamine Pyrophosphate 152-156 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 33-36 16472748-2 2006 We have determined the crystal structures of E1b with ThDP bound intermediates after decarboxylation of alpha-ketoacids. Thiamine Pyrophosphate 54-58 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 45-48 16472748-3 2006 We show that a key tyrosine residue in the E1b active site functions as a conformational switch to reduce the reactivity of the ThDP cofactor through interactions with its thiazolium ring. Thiamine Pyrophosphate 128-132 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 43-46 16786225-1 2006 The identification of 2-hydroxyphytanoyl-CoA lyase (2-HPCL), a thiamine pyrophosphate (TPP)-dependent peroxisomal enzyme involved in the alpha-oxidation of phytanic acid and of 2-hydroxy straight chain fatty acids, pointed towards a role of TPP in these processes. Thiamine Pyrophosphate 241-244 2-hydroxyacyl-CoA lyase 1 Homo sapiens 22-50 16786225-1 2006 The identification of 2-hydroxyphytanoyl-CoA lyase (2-HPCL), a thiamine pyrophosphate (TPP)-dependent peroxisomal enzyme involved in the alpha-oxidation of phytanic acid and of 2-hydroxy straight chain fatty acids, pointed towards a role of TPP in these processes. Thiamine Pyrophosphate 241-244 2-hydroxyacyl-CoA lyase 1 Homo sapiens 52-58 14623876-2 2004 This unusual N-C bond forming reaction is catalyzed by the thiamin diphosphate (ThP2)-dependent enzyme N2-(2-carboxyethyl)arginine synthase. Thiamine Pyrophosphate 59-78 GLI family zinc finger 2 Homo sapiens 80-84 15802905-1 2005 OBJECTIVE: To report the first adult case of hypophosphatasia and absence of intestinal alkaline phosphatase (ALP) isoenzymes associated with a low level of red blood cell thiamine pyrophosphate. Thiamine Pyrophosphate 172-194 alkaline phosphatase, placental Homo sapiens 110-113 15640355-1 2005 The thiamin diphosphate (ThDP)-dependent enzyme acetohydroxyacid synthase (AHAS) catalyzes the first common step in branched-chain amino acid biosynthesis. Thiamine Pyrophosphate 4-23 ilvB acetolactate synthase like Homo sapiens 75-79 15640355-1 2005 The thiamin diphosphate (ThDP)-dependent enzyme acetohydroxyacid synthase (AHAS) catalyzes the first common step in branched-chain amino acid biosynthesis. Thiamine Pyrophosphate 25-29 ilvB acetolactate synthase like Homo sapiens 75-79 15511224-5 2004 The influence of the donor substrate on the coenzyme-apotransketolase interaction was predicted as a result of formation of the transketolase reaction intermediate 2-(alpha,beta-dihydroxyethyl)-thiamin diphosphate, which exhibited a higher affinity to the enzyme than thiamin diphosphate. Thiamine Pyrophosphate 194-213 transketolase Homo sapiens 56-69 15511224-6 2004 The enhancement of thiamin diphosphate"s affinity to apotransketolase in the presence of donor substrate is probably one of the mechanisms underlying the substrate-affected transketolase regulation at low coenzyme concentrations. Thiamine Pyrophosphate 19-38 transketolase Homo sapiens 56-69 15514058-4 2004 We have identified a partial loss-of-function mutation in the Caenorhabditis elegans gene (tpk-1) that encodes thiamine pyrophosphokinase, which forms TPP from thiamine at the expense of ATP inside cells. Thiamine Pyrophosphate 151-154 thiamine pyrophosphokinase 1 Caenorhabditis elegans 91-96 15166214-1 2004 The decarboxylase/dehydrogenase (E1b) component of the 4-megadalton human branched-chain alpha-keto acid dehydrogenase (BCKD) metabolic machine is a thiamin diphosphate (ThDP)-dependent enzyme with a heterotetrameric cofactor-binding fold. Thiamine Pyrophosphate 149-168 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 33-36 15166214-1 2004 The decarboxylase/dehydrogenase (E1b) component of the 4-megadalton human branched-chain alpha-keto acid dehydrogenase (BCKD) metabolic machine is a thiamin diphosphate (ThDP)-dependent enzyme with a heterotetrameric cofactor-binding fold. Thiamine Pyrophosphate 170-174 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 33-36 15166214-3 2004 In the present study, we show that the binding of cofactor ThDP to the E1b active site induces a disorder-to-order transition of the conserved phosphorylation loop carrying the two phosphorylation sites Ser(292)-alpha and Ser(302)-alpha, as deduced from the 1.80-1.85 A apoE1b and holoE1b structures. Thiamine Pyrophosphate 59-63 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 71-74 15166214-7 2004 ThDP binding that orders the loop prevents phosphorylation of E1b by the BCKD kinase and averts the inactivation of wild-type E1b, but not the above mutants, by this covalent modification. Thiamine Pyrophosphate 0-4 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 62-65 15166214-7 2004 ThDP binding that orders the loop prevents phosphorylation of E1b by the BCKD kinase and averts the inactivation of wild-type E1b, but not the above mutants, by this covalent modification. Thiamine Pyrophosphate 0-4 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 126-129 14527822-3 2003 The present study investigated a putative modulation of MPP+ intestinal apical uptake and ecto-ALP activity by thiamine (T+) and thiamine pyrophosphate (TPP, a T+ dietary precursor). Thiamine Pyrophosphate 129-151 alkaline phosphatase, placental Homo sapiens 95-98 14527822-6 2003 T+ and TPP were able to increase ecto-ALP activity, with an equal potency, and to decrease 3H-MPP+ apical uptake, with a similar potency. Thiamine Pyrophosphate 7-10 tripartite motif containing 33 Homo sapiens 33-37 14527822-6 2003 T+ and TPP were able to increase ecto-ALP activity, with an equal potency, and to decrease 3H-MPP+ apical uptake, with a similar potency. Thiamine Pyrophosphate 7-10 alkaline phosphatase, placental Homo sapiens 38-41 14527822-8 2003 The results suggest that the effect of T+ and TPP on ecto-ALP activity may lead to inhibition of the intestinal absorption of other organic cations present in the diet. Thiamine Pyrophosphate 46-49 tripartite motif containing 33 Homo sapiens 53-57 14527822-8 2003 The results suggest that the effect of T+ and TPP on ecto-ALP activity may lead to inhibition of the intestinal absorption of other organic cations present in the diet. Thiamine Pyrophosphate 46-49 alkaline phosphatase, placental Homo sapiens 58-61 12379473-3 2002 A thiamine diphosphate (ThDP) kinase (ThDP+ATP if ThTP+ADP) has been purified from brewer"s yeast and shown to exist in rat liver. Thiamine Pyrophosphate 24-28 thiamine triphosphatase Homo sapiens 50-54 12902323-1 2003 We report here that alterations of either His291-alpha or His146-beta" in the active site of human branched-chain alpha-ketoacid dehydrogenase (E1b) impede both the decarboxylation and the reductive acylation reactions catalyzed by E1b as well as the binding of cofactor thiamin diphosphate (ThDP). Thiamine Pyrophosphate 271-290 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 144-147 12902323-1 2003 We report here that alterations of either His291-alpha or His146-beta" in the active site of human branched-chain alpha-ketoacid dehydrogenase (E1b) impede both the decarboxylation and the reductive acylation reactions catalyzed by E1b as well as the binding of cofactor thiamin diphosphate (ThDP). Thiamine Pyrophosphate 271-290 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 232-235 12902323-1 2003 We report here that alterations of either His291-alpha or His146-beta" in the active site of human branched-chain alpha-ketoacid dehydrogenase (E1b) impede both the decarboxylation and the reductive acylation reactions catalyzed by E1b as well as the binding of cofactor thiamin diphosphate (ThDP). Thiamine Pyrophosphate 292-296 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 144-147 12902323-1 2003 We report here that alterations of either His291-alpha or His146-beta" in the active site of human branched-chain alpha-ketoacid dehydrogenase (E1b) impede both the decarboxylation and the reductive acylation reactions catalyzed by E1b as well as the binding of cofactor thiamin diphosphate (ThDP). Thiamine Pyrophosphate 292-296 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 232-235 12902323-3 2003 The imidazole ring of His291-alpha in E1b coordinates to the terminal phosphate oxygen atoms of bound ThDP. Thiamine Pyrophosphate 102-106 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 38-41 12902323-7 2003 Our results suggest that: 1) His291-alpha plays a structural rather than a catalytic role in the binding of cofactor ThDP and the lipoyl-bearing domain to E1b, and 2) His146-beta" is an essential catalytic residue, probably functioning as a proton donor in the reductive acylation of lipoamide on the lipoyl-bearing domain. Thiamine Pyrophosphate 117-121 branched chain keto acid dehydrogenase E1 subunit beta Homo sapiens 155-158 12693972-8 2003 According to our hypothesis, the induced absorption band is that of the imino form of ThDP resulting from three contributing features of the ThDP binding site of transketolase: the relative hydrophobicity of this site, hydrogen bonding of the N1;-atom of the ThDP aminopyrimidine ring to Glu418, and base stacking interactions between the aminopyrimidine ring of ThDP and Phe445. Thiamine Pyrophosphate 86-90 transketolase Homo sapiens 162-175 12693972-8 2003 According to our hypothesis, the induced absorption band is that of the imino form of ThDP resulting from three contributing features of the ThDP binding site of transketolase: the relative hydrophobicity of this site, hydrogen bonding of the N1;-atom of the ThDP aminopyrimidine ring to Glu418, and base stacking interactions between the aminopyrimidine ring of ThDP and Phe445. Thiamine Pyrophosphate 141-145 transketolase Homo sapiens 162-175 12693972-8 2003 According to our hypothesis, the induced absorption band is that of the imino form of ThDP resulting from three contributing features of the ThDP binding site of transketolase: the relative hydrophobicity of this site, hydrogen bonding of the N1;-atom of the ThDP aminopyrimidine ring to Glu418, and base stacking interactions between the aminopyrimidine ring of ThDP and Phe445. Thiamine Pyrophosphate 141-145 transketolase Homo sapiens 162-175 12693972-8 2003 According to our hypothesis, the induced absorption band is that of the imino form of ThDP resulting from three contributing features of the ThDP binding site of transketolase: the relative hydrophobicity of this site, hydrogen bonding of the N1;-atom of the ThDP aminopyrimidine ring to Glu418, and base stacking interactions between the aminopyrimidine ring of ThDP and Phe445. Thiamine Pyrophosphate 141-145 transketolase Homo sapiens 162-175 12376536-2 2002 Vitamin B(1) in its active form thiamin pyrophosphate is an essential coenzyme that is synthesized by coupling of pyrimidine (hydroxymethylpyrimidine; HMP) and thiazole (hydroxyethylthiazole) moieties in bacteria. Thiamine Pyrophosphate 32-53 inner membrane mitochondrial protein Homo sapiens 151-154 12227466-0 2002 A novel Y243S mutation in the pyruvate dehydrogenase El alpha gene subunit: correlation with thiamine pyrophosphate interaction. Thiamine Pyrophosphate 93-115 apelin receptor early endogenous ligand Homo sapiens 53-61 11960673-8 2002 Transketolase is dependent on thiamine pyrophosphate as a cofactor, and therefore, the decreased enzyme activity could be the result of an interaction between furazolidone and thiamine metabolism. Thiamine Pyrophosphate 30-52 transketolase like 1 Gallus gallus 0-13 11038362-3 2001 However, high level RFC1 expression substantially reduced accumulation of the active thiamin coenzyme, thiamin pyrophosphate (TPP). Thiamine Pyrophosphate 103-124 replication factor C (activator 1) 1 Mus musculus 20-24 11535779-3 2001 The ilv2-thi1 allele encodes a functional AHAS enzyme with an altered dependence for the cofactor TPP resulting in the thiamin auxotrophic phenotype. Thiamine Pyrophosphate 98-101 acetolactate synthase catalytic subunit Saccharomyces cerevisiae S288C 4-8 11535779-3 2001 The ilv2-thi1 allele encodes a functional AHAS enzyme with an altered dependence for the cofactor TPP resulting in the thiamin auxotrophic phenotype. Thiamine Pyrophosphate 98-101 acetolactate synthase catalytic subunit Saccharomyces cerevisiae S288C 9-13 11736629-1 2001 Transketolase is the simplest representative of the thiamine diphosphate-dependent enzymes. Thiamine Pyrophosphate 52-72 transketolase Homo sapiens 0-13 11485553-8 2001 Secondly, thiamin pyrophosphate markedly decreases the amount of phosphate that PDK1 incorporates in sites 2 and 3 and that PDK2 incorporates in site 2. Thiamine Pyrophosphate 10-31 pyruvate dehydrogenase kinase 1 Homo sapiens 80-84 11485553-8 2001 Secondly, thiamin pyrophosphate markedly decreases the amount of phosphate that PDK1 incorporates in sites 2 and 3 and that PDK2 incorporates in site 2. Thiamine Pyrophosphate 10-31 pyruvate dehydrogenase kinase 2 Homo sapiens 124-128 11488910-3 2001 Thiamine (vitamin B1) is metabolized to thiamine pyrophosphate, the cofactor of transketolase, which is involved in ribose synthesis, necessary for cell replication. Thiamine Pyrophosphate 40-62 transketolase Mus musculus 80-93 11038362-3 2001 However, high level RFC1 expression substantially reduced accumulation of the active thiamin coenzyme, thiamin pyrophosphate (TPP). Thiamine Pyrophosphate 126-129 replication factor C (activator 1) 1 Mus musculus 20-24 11038362-4 2001 This decreased level of TPP, synthesized intracellularly from imported thiamin, resulted from RFC1-mediated efflux of TPP. Thiamine Pyrophosphate 24-27 replication factor C (activator 1) 1 Mus musculus 94-98 11038362-4 2001 This decreased level of TPP, synthesized intracellularly from imported thiamin, resulted from RFC1-mediated efflux of TPP. Thiamine Pyrophosphate 118-121 replication factor C (activator 1) 1 Mus musculus 94-98 11038362-6 2001 (i) Efflux of intracellular TPP was increased in cells with high expression of RFC1. Thiamine Pyrophosphate 28-31 replication factor C (activator 1) 1 Mus musculus 79-83 10409709-4 1999 The activity was equally high toward ADP/ATP, GDP/GTP, and UDP/UTP and approximately 50% less toward CDP/CTP and thiamine pyrophosphate, but there was no activity toward GMP, indicating that the Ynd1 protein belongs to the apyrase family. Thiamine Pyrophosphate 113-135 apyrase Saccharomyces cerevisiae S288C 195-199 10468558-0 1999 Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during alpha-oxidation of 3-methyl-branched fatty acids. Thiamine Pyrophosphate 95-117 2-hydroxyacyl-CoA lyase 1 Homo sapiens 51-79 9194907-4 1997 Transketolase is a homodimeric enzyme containing two molecules of noncovalently bound thiamine pyrophosphate. Thiamine Pyrophosphate 86-108 transketolase Homo sapiens 0-13 10466187-2 1999 It has been suggested that the Wernicke-Korsakoff syndrome is associated with a genetic variant of transketolase which requires a higher than normal concentration of thiamin diphosphate for activity. Thiamine Pyrophosphate 166-185 transketolase Homo sapiens 99-112 9933732-5 1999 The effect of exogenous thiamine pyrophosphate (TPP) addition on the blood transketolase (TK) activity (TPP TK effect) served to estimate thiamine deficiency. Thiamine Pyrophosphate 24-46 transketolase Homo sapiens 75-88 9933732-5 1999 The effect of exogenous thiamine pyrophosphate (TPP) addition on the blood transketolase (TK) activity (TPP TK effect) served to estimate thiamine deficiency. Thiamine Pyrophosphate 24-46 transketolase Homo sapiens 90-92 9933732-5 1999 The effect of exogenous thiamine pyrophosphate (TPP) addition on the blood transketolase (TK) activity (TPP TK effect) served to estimate thiamine deficiency. Thiamine Pyrophosphate 48-51 transketolase Homo sapiens 75-88 9933732-5 1999 The effect of exogenous thiamine pyrophosphate (TPP) addition on the blood transketolase (TK) activity (TPP TK effect) served to estimate thiamine deficiency. Thiamine Pyrophosphate 48-51 transketolase Homo sapiens 90-92 9933732-5 1999 The effect of exogenous thiamine pyrophosphate (TPP) addition on the blood transketolase (TK) activity (TPP TK effect) served to estimate thiamine deficiency. Thiamine Pyrophosphate 104-107 transketolase Homo sapiens 75-88 9933732-5 1999 The effect of exogenous thiamine pyrophosphate (TPP) addition on the blood transketolase (TK) activity (TPP TK effect) served to estimate thiamine deficiency. Thiamine Pyrophosphate 104-107 transketolase Homo sapiens 90-92 9933732-9 1999 RESULTS: Of 118 inpatients, 46 (39%) presented with a TPP TK effect of >15%, and 6 with values of >22%, indicating moderate and severe thiamine deficiency, respectively. Thiamine Pyrophosphate 54-57 transketolase Homo sapiens 58-60 9933732-10 1999 Only 6 of 30 outpatients (20%) exhibited a TPP TK effect of >15% and none of them reached values of >18%. Thiamine Pyrophosphate 43-46 transketolase Homo sapiens 47-49 9924800-5 1998 In the homodimer transketolase the two catalytic sites, where dihydroxyethyl groups are transferred from ketose donors to aldose acceptors, are formed at the interface between the two subunits, where the thiazole and pyrimidine rings of thiamin diphosphate are bound. Thiamine Pyrophosphate 237-256 transketolase Homo sapiens 17-30 9655911-3 1998 Thiamin diphosphate (ThDP)-dependent enzymes vary in their substrate tolerance from rather strict substrate specificity (phosphoketolases, glyoxylate carboligase) to more permissive enzymes (transketolase, dihydroxyacetone synthase, pyruvate decarboxylase) and therefore differ in their potential to be used as biocatalysts. Thiamine Pyrophosphate 0-19 transketolase Homo sapiens 191-204 9655911-3 1998 Thiamin diphosphate (ThDP)-dependent enzymes vary in their substrate tolerance from rather strict substrate specificity (phosphoketolases, glyoxylate carboligase) to more permissive enzymes (transketolase, dihydroxyacetone synthase, pyruvate decarboxylase) and therefore differ in their potential to be used as biocatalysts. Thiamine Pyrophosphate 21-25 transketolase Homo sapiens 191-204 9235906-8 1997 Northern blot analysis demonstrated that THI10 gene expression is regulated at the mRNA level by intracellular thiamin pyrophosphate and that it requires a positive regulatory factor encoded by THI3 gene. Thiamine Pyrophosphate 111-132 thiamine transporter THI7 Saccharomyces cerevisiae S288C 41-46 10622704-2 1999 p-Hydroxyphenylpyruvate proved to be a reversible and competitive inhibitor of transketolase with respect to substrate; it was also able to displace thiamine diphosphate from holotransketolase. Thiamine Pyrophosphate 149-169 transketolase Homo sapiens 79-92 10231381-17 1999 The mutation in pdc1-14 (Ser455Phe) is located within the ThDP fold and is likely to affect binding and/or orientation of the cofactor in the protein. Thiamine Pyrophosphate 58-62 indolepyruvate decarboxylase 1 Saccharomyces cerevisiae S288C 16-23 9924800-1 1998 This review highlights recent research on the properties and functions of the enzyme transketolase, which requires thiamin diphosphate and a divalent metal ion for its activity. Thiamine Pyrophosphate 115-134 transketolase Homo sapiens 85-98 9924800-3 1998 Yeast transketolase is one of several thiamin diphosphate dependent enzymes whose three-dimensional structures have been determined. Thiamine Pyrophosphate 38-57 transketolase Homo sapiens 6-19 9611778-1 1998 Transketolase belongs to the family of thiamin diphosphate dependent enzymes. Thiamine Pyrophosphate 39-58 transketolase Homo sapiens 0-13 9357955-0 1997 Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation. Thiamine Pyrophosphate 54-74 transketolase Homo sapiens 23-36 9357955-1 1997 Active human transketolase is a homodimeric enzyme possessing two active sites, each with a non-covalently bound thiamine diphosphate and magnesium. Thiamine Pyrophosphate 113-133 transketolase Homo sapiens 13-26 9194907-6 1997 Previous findings demonstrated that purified his-transketolase had a Km app for cofactor and a thiamine pyrophosphate-dependent lag period for attaining steady-state kinetics that was similar to transketolase purified from human tissues. Thiamine Pyrophosphate 95-117 transketolase Homo sapiens 49-62 9134192-4 1997 The parenteral administration of cytochrome c with flavin mononucleotide and thiamine diphosphate abolished the intolerable pain. Thiamine Pyrophosphate 77-97 cytochrome c, somatic Homo sapiens 33-45 8683340-5 1996 When exogenous thiamine pyrophosphate was added to the activity assays, differences between transketolase and alpha-KGDH became readily apparent. Thiamine Pyrophosphate 15-37 transketolase Homo sapiens 92-105 8974135-3 1996 The vitamin B1 level was determined as thiamine pyrophosphate effect on transketolase activity in red blood cell lysates. Thiamine Pyrophosphate 39-61 transketolase Homo sapiens 72-85 8980789-1 1996 Thiamine diphosphate (TDP) is an important cofactor of pyruvate (PDH) and alpha-ketoglutarate (KGDH) dehydrogenases and transketolase. Thiamine Pyrophosphate 0-20 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 65-68 9201534-3 1997 Postmortem results revealed low erythrocyte transketolase activity, which was increased by 22% by in vitro addition of thiamine diphosphate (TDP effect). Thiamine Pyrophosphate 119-139 transketolase Homo sapiens 44-57 8780344-3 1996 Measurements of urinary thiamine and blood transketolase and its activation with thiamine pyrophosphate were made. Thiamine Pyrophosphate 81-103 transketolase Homo sapiens 43-56 7861245-1 1995 We have investigated the hysteretic properties of human transketolase with emphasis on its dependency on thiamine pyrophosphate concentration. Thiamine Pyrophosphate 105-127 transketolase Homo sapiens 56-69 8815392-2 1996 Studies in autopsied brain tissue from neuropathologically proven AD patients reveal significantly reduced activities of the thiamine phosphate dephosphorylating enzymes thiamine diphosphatase (TDPase) and thiamine monophosphatase (TMPase) as well as the thiamine diphosphate-dependent enzymes, pyruvate dehydrogenase complex, alpha-ketoglutarate dehydrogenase (alpha KGDH) and transketolase. Thiamine Pyrophosphate 255-275 acid phosphatase 3 Homo sapiens 232-238 7548453-1 1995 The indirect estimation of thiamine levels in human blood by measuring thiamine pyrophosphate effect on erythrocyte transketolase activity is the method of choice in most clinical laboratories. Thiamine Pyrophosphate 71-93 transketolase Homo sapiens 116-129 7596328-3 1995 In particular, Tk derived from fibroblasts has been found to have an increased Km app for its cofactor thiamine pyrophosphate [TPP] and/or exist in different isoelectric forms in alcoholic patients with WKS as compared with unaffected individuals. Thiamine Pyrophosphate 103-125 transketolase Homo sapiens 15-17 7596328-3 1995 In particular, Tk derived from fibroblasts has been found to have an increased Km app for its cofactor thiamine pyrophosphate [TPP] and/or exist in different isoelectric forms in alcoholic patients with WKS as compared with unaffected individuals. Thiamine Pyrophosphate 127-130 transketolase Homo sapiens 15-17 7861245-4 1995 At physiological thiamine pyrophosphate concentrations, the inverse rate constant was in the range of 10 to 20 min for fibroblast-derived transketolase and increased dramatically with only small decreases from these levels of thiamine pyrophosphate. Thiamine Pyrophosphate 17-39 transketolase Homo sapiens 138-151 7861245-4 1995 At physiological thiamine pyrophosphate concentrations, the inverse rate constant was in the range of 10 to 20 min for fibroblast-derived transketolase and increased dramatically with only small decreases from these levels of thiamine pyrophosphate. Thiamine Pyrophosphate 226-248 transketolase Homo sapiens 138-151 8279670-2 1993 Results of this study demonstrate significant reductions of TPP-dependent enzymes [pyruvate dehydrogenase complex, alpha-ketoglutarate dehydrogenase (alpha KGDH), and transketolase] in autopsied cerebellar vermis samples from alcoholic patients with the clinical and neuropathologically confirmed diagnosis of Wernicke-Korsakoff Syndrome (WKS). Thiamine Pyrophosphate 60-63 transketolase Homo sapiens 167-180 7982968-11 1994 Northern blot analysis demonstrated that THI6 gene expression is regulated at the mRNA level by intracellular thiamin pyrophosphate, a coenzyme form of thiamin, and that it requires the positive regulatory factors encoded by the THI2 and THI3 genes. Thiamine Pyrophosphate 110-131 bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase Saccharomyces cerevisiae S288C 41-45 8243472-5 1993 The best estimate of the apparent Km, 1.59 +/- 0.23 microM, for the binding of Mg(2+)-thiamin diphosphate to transketolase was obtained in the presence of a high non-inhibitory concentration of magnesium and varied concentrations of thiamin diphosphate. Thiamine Pyrophosphate 86-105 transketolase Homo sapiens 109-122 8069629-0 1993 A thiamin diphosphate binding fold revealed by comparison of the crystal structures of transketolase, pyruvate oxidase and pyruvate decarboxylase. Thiamine Pyrophosphate 2-21 transketolase Homo sapiens 87-100 8186256-3 1994 Only 3-5% of total thiamine diphosphate was bound to transketolase, a cytosolic enzyme. Thiamine Pyrophosphate 19-39 transketolase Rattus norvegicus 53-66 8974347-3 1994 We have investigated the interactions of the thiamine-utilizing enzyme transketolase [Tk], derived from human fibroblasts, lymphoblasts, and various brain regions, with its cofactor, thiamine pyrophosphate [TPP], in an attempt to elucidate the molecular basis of selective brain damage in alcoholism-associated thiamine deficiency. Thiamine Pyrophosphate 183-205 transketolase Homo sapiens 71-84 8974347-3 1994 We have investigated the interactions of the thiamine-utilizing enzyme transketolase [Tk], derived from human fibroblasts, lymphoblasts, and various brain regions, with its cofactor, thiamine pyrophosphate [TPP], in an attempt to elucidate the molecular basis of selective brain damage in alcoholism-associated thiamine deficiency. Thiamine Pyrophosphate 183-205 transketolase Homo sapiens 86-88 8974347-3 1994 We have investigated the interactions of the thiamine-utilizing enzyme transketolase [Tk], derived from human fibroblasts, lymphoblasts, and various brain regions, with its cofactor, thiamine pyrophosphate [TPP], in an attempt to elucidate the molecular basis of selective brain damage in alcoholism-associated thiamine deficiency. Thiamine Pyrophosphate 207-210 transketolase Homo sapiens 71-84 8974347-3 1994 We have investigated the interactions of the thiamine-utilizing enzyme transketolase [Tk], derived from human fibroblasts, lymphoblasts, and various brain regions, with its cofactor, thiamine pyrophosphate [TPP], in an attempt to elucidate the molecular basis of selective brain damage in alcoholism-associated thiamine deficiency. Thiamine Pyrophosphate 207-210 transketolase Homo sapiens 86-88 1499340-2 1992 Thiamin pyrophosphate catalyzes various decarboxylation and transfer reactions involving ketone groups because the thiazolium ring with its positively charged N atom can, on the loss of a proton from the adjacent C-2, generate a ylid which adds to carbonyl groups to produce a substrate ylid. Thiamine Pyrophosphate 0-21 complement C2 Homo sapiens 213-216 1394639-3 1992 The enzyme catalyzed the hydrolysis of adenosine 5"-triphosphate, adenosine 5"-diphosphate, thiamine pyrophosphate, inorganic pyrophosphate, and phosphoprotein such as casein and phosvitin, but not of several phosphomonoesters, except for p-nitrophenyl phosphate and o-phosphotyrosine. Thiamine Pyrophosphate 92-114 casein kinase 2 beta Rattus norvegicus 179-188 8394343-8 1993 A gene disruption experiment demonstrated that the THI80 gene was essential for growth, and therefore, revealed that TPK is the only enzyme capable of synthesizing thiamin pyrophosphate in yeast. Thiamine Pyrophosphate 164-185 thiamine diphosphokinase Saccharomyces cerevisiae S288C 51-56 8394343-9 1993 Studies of Northern blot analysis and the enzyme assay demonstrated that the THI80 gene expression is regulated mainly at the mRNA level by the intracellular thiamin pyrophosphate and requires the positive regulatory factors encoded by THI2 and THI3 genes. Thiamine Pyrophosphate 158-179 thiamine diphosphokinase Saccharomyces cerevisiae S288C 77-82 8394343-9 1993 Studies of Northern blot analysis and the enzyme assay demonstrated that the THI80 gene expression is regulated mainly at the mRNA level by the intracellular thiamin pyrophosphate and requires the positive regulatory factors encoded by THI2 and THI3 genes. Thiamine Pyrophosphate 158-179 branched-chain-2-oxoacid decarboxylase THI3 Saccharomyces cerevisiae S288C 245-249 8419340-1 1993 Variants of the enzyme transketolase which possess reduced affinity for its cofactor thiamine pyrophosphate (high apparent Km) have been described in chronic alcoholic patients with Wernicke-Korsakoff syndrome. Thiamine Pyrophosphate 85-107 transketolase Homo sapiens 23-36 8012500-3 1993 In addition to NAD+, thiamine pyrophosphate and coenzyme A in the incubation mixture, a preparation of NADH:cytochrome c reductase (NADHCR) together with cytochrome c has a stimulating effect on the PDHC activity. Thiamine Pyrophosphate 21-43 cytochrome c, somatic Homo sapiens 108-120 8012500-3 1993 In addition to NAD+, thiamine pyrophosphate and coenzyme A in the incubation mixture, a preparation of NADH:cytochrome c reductase (NADHCR) together with cytochrome c has a stimulating effect on the PDHC activity. Thiamine Pyrophosphate 21-43 cytochrome c, somatic Homo sapiens 154-166 1297775-2 1992 The activities of 3 TPP-dependent enzymes are reduced in DAT brain: transketolase (TK), the pyruvate dehydrogenase complex (PDHC), and the alpha-ketoglutarate dehydrogenase complex (KGDHC). Thiamine Pyrophosphate 20-23 transketolase Homo sapiens 83-85 2229026-1 1990 We showed previously that cytosolic adenylate kinase (AK1) purified from pig skeletal muscle catalyzes in vitro formation of thiamin triphosphate (TTP) from thiamin diphosphate (TDP) and ADP in addition to ATP formation from ADP [Shikata, H. et al. Thiamine Pyrophosphate 157-176 adenylate kinase 1 Sus scrofa 54-57 1297775-2 1992 The activities of 3 TPP-dependent enzymes are reduced in DAT brain: transketolase (TK), the pyruvate dehydrogenase complex (PDHC), and the alpha-ketoglutarate dehydrogenase complex (KGDHC). Thiamine Pyrophosphate 20-23 solute carrier family 6 member 3 Homo sapiens 57-60 1297775-2 1992 The activities of 3 TPP-dependent enzymes are reduced in DAT brain: transketolase (TK), the pyruvate dehydrogenase complex (PDHC), and the alpha-ketoglutarate dehydrogenase complex (KGDHC). Thiamine Pyrophosphate 20-23 transketolase Homo sapiens 68-81 1807869-1 1991 In 60 thiamine deficient patients, the mean erythrocyte transketolase activity after activation by thiamine diphosphate cofactor in vitro, representing the apparent sum of holoenzyme and apoenzyme activities, was 0.609 (SD 0.166) U/g Hb before thiamine therapy and rose to 0.772 (SD 0.152) U/g Hb immediately after the administration of thiamine to the patients. Thiamine Pyrophosphate 99-119 transketolase Homo sapiens 56-69 1807869-2 1991 The difference between these values, 0.163 (SD 0.130) U/g, is the mean activity of transketolase protein which can be activated by thiamine in vivo but not by thiamine diphosphate in vitro. Thiamine Pyrophosphate 159-179 transketolase Homo sapiens 83-96 2211597-3 1990 The Kis, Kid, and Kin for thiamin pyrophosphate were in the range of 0.009 to 5.1 microM over the range of K+ concentrations tested. Thiamine Pyrophosphate 26-47 Kin17 DNA and RNA binding protein Bos taurus 18-21 2210959-0 1990 The relationship between the thiamin pyrophosphate effect and the saturation status of the transketolase with its coenzyme in human erythrocytes. Thiamine Pyrophosphate 29-50 transketolase Homo sapiens 91-104 2307803-3 1990 Each of three individuals responded clinically to the administration of thiamin tetrahydrofurfuryl disulfide (TTFD), and erythrocyte transketolase (TKA) became fully saturated with thiamin pyrophosphate (TPP). Thiamine Pyrophosphate 181-202 transketolase Homo sapiens 148-151 2307803-3 1990 Each of three individuals responded clinically to the administration of thiamin tetrahydrofurfuryl disulfide (TTFD), and erythrocyte transketolase (TKA) became fully saturated with thiamin pyrophosphate (TPP). Thiamine Pyrophosphate 204-207 transketolase Homo sapiens 148-151 2210959-2 1990 But there has not been any report concerning whether or not the thiamin pyrophosphate effect really reflects the saturation status of transketolase with thiamin pyrophosphate. Thiamine Pyrophosphate 153-174 transketolase Homo sapiens 134-147 2210959-3 1990 In this report we studied the relationship between the thiamin pyrophosphate effect and the saturation status of transketolase. Thiamine Pyrophosphate 55-76 transketolase Homo sapiens 113-126 2210959-5 1990 The molar ratio of thiamin pyrophosphate to transketolase was in inverse proportion to the thiamin pyrophosphate effect. Thiamine Pyrophosphate 19-40 transketolase Homo sapiens 44-57 2210959-5 1990 The molar ratio of thiamin pyrophosphate to transketolase was in inverse proportion to the thiamin pyrophosphate effect. Thiamine Pyrophosphate 91-112 transketolase Homo sapiens 44-57 2210959-8 1990 These results indicate that the thiamin pyrophosphate effect really reflects the saturation status of transketolase with coenzyme. Thiamine Pyrophosphate 32-53 transketolase Homo sapiens 102-115 34942318-1 2022 BACKGROUND: Thiamine diphosphate (ThDP), an indispensable cofactor for oxidative energy metabolism, is synthesized through the reaction thiamine + ATP ThDP + AMP, catalyzed by thiamine pyrophosphokinase 1 (TPK1), a cytosolic dimeric enzyme. Thiamine Pyrophosphate 12-32 thiamin pyrophosphokinase 1 Homo sapiens 178-206 34942318-1 2022 BACKGROUND: Thiamine diphosphate (ThDP), an indispensable cofactor for oxidative energy metabolism, is synthesized through the reaction thiamine + ATP ThDP + AMP, catalyzed by thiamine pyrophosphokinase 1 (TPK1), a cytosolic dimeric enzyme. Thiamine Pyrophosphate 12-32 thiamin pyrophosphokinase 1 Homo sapiens 208-212 34942318-1 2022 BACKGROUND: Thiamine diphosphate (ThDP), an indispensable cofactor for oxidative energy metabolism, is synthesized through the reaction thiamine + ATP ThDP + AMP, catalyzed by thiamine pyrophosphokinase 1 (TPK1), a cytosolic dimeric enzyme. Thiamine Pyrophosphate 34-38 thiamin pyrophosphokinase 1 Homo sapiens 178-206 34942318-1 2022 BACKGROUND: Thiamine diphosphate (ThDP), an indispensable cofactor for oxidative energy metabolism, is synthesized through the reaction thiamine + ATP ThDP + AMP, catalyzed by thiamine pyrophosphokinase 1 (TPK1), a cytosolic dimeric enzyme. Thiamine Pyrophosphate 34-38 thiamin pyrophosphokinase 1 Homo sapiens 208-212 34942318-1 2022 BACKGROUND: Thiamine diphosphate (ThDP), an indispensable cofactor for oxidative energy metabolism, is synthesized through the reaction thiamine + ATP ThDP + AMP, catalyzed by thiamine pyrophosphokinase 1 (TPK1), a cytosolic dimeric enzyme. Thiamine Pyrophosphate 153-157 thiamin pyrophosphokinase 1 Homo sapiens 178-206 34942318-1 2022 BACKGROUND: Thiamine diphosphate (ThDP), an indispensable cofactor for oxidative energy metabolism, is synthesized through the reaction thiamine + ATP ThDP + AMP, catalyzed by thiamine pyrophosphokinase 1 (TPK1), a cytosolic dimeric enzyme. Thiamine Pyrophosphate 153-157 thiamin pyrophosphokinase 1 Homo sapiens 208-212 34942318-9 2022 GENERAL SIGNIFICANCE: Inhibition of TPK1 by ThDP explains why intracellular ThDP levels remain low after administration of even very high doses of thiamine. Thiamine Pyrophosphate 44-48 thiamine pyrophosphokinase Mus musculus 36-40 34587972-8 2021 We found that mitochondrial TPP levels were significantly decreased in the presence of SLC25A19 variants, indicating that TPP transport activities of mutated SLC25A19 proteins were impaired. Thiamine Pyrophosphate 28-31 solute carrier family 25 member 19 Homo sapiens 87-95 2136519-9 1990 In assaying for red-cell transketolase levels, this subgroup showed higher thiamin pyrophosphate effects than did the whole sample. Thiamine Pyrophosphate 75-96 transketolase Homo sapiens 25-38 34998824-3 2022 We showed hypoxia causes significant inhibition in TPP and free thiamin uptake by colonic NCM460 cells and by colonoid monolayers; however, it also caused a significant reduction in the expression of TPP (SLC44A4) and free thiamin (SLC19A2 and SLC19A3) transporters, and in activity of their gene promoters. Thiamine Pyrophosphate 200-203 solute carrier family 44 member 4 Homo sapiens 205-212 34587972-8 2021 We found that mitochondrial TPP levels were significantly decreased in the presence of SLC25A19 variants, indicating that TPP transport activities of mutated SLC25A19 proteins were impaired. Thiamine Pyrophosphate 28-31 solute carrier family 25 member 19 Homo sapiens 158-166 34587972-8 2021 We found that mitochondrial TPP levels were significantly decreased in the presence of SLC25A19 variants, indicating that TPP transport activities of mutated SLC25A19 proteins were impaired. Thiamine Pyrophosphate 122-125 solute carrier family 25 member 19 Homo sapiens 87-95 34587972-8 2021 We found that mitochondrial TPP levels were significantly decreased in the presence of SLC25A19 variants, indicating that TPP transport activities of mutated SLC25A19 proteins were impaired. Thiamine Pyrophosphate 122-125 solute carrier family 25 member 19 Homo sapiens 158-166 35156781-16 2022 RESULTS: Thiamine pyrophosphate significantly decreased the cyclophosphamide-induced increase in the levels of the oxidant parameter malondialdehyde (MDA), proinflammatory nuclear factor kappa B (NF-kappaB), tumor necrosis factor alpha (TNF-alpha), and interleukin 1 beta (IL-1beta). Thiamine Pyrophosphate 9-31 tumor necrosis factor Rattus norvegicus 208-235 35156781-16 2022 RESULTS: Thiamine pyrophosphate significantly decreased the cyclophosphamide-induced increase in the levels of the oxidant parameter malondialdehyde (MDA), proinflammatory nuclear factor kappa B (NF-kappaB), tumor necrosis factor alpha (TNF-alpha), and interleukin 1 beta (IL-1beta). Thiamine Pyrophosphate 9-31 tumor necrosis factor Rattus norvegicus 237-246 35156781-16 2022 RESULTS: Thiamine pyrophosphate significantly decreased the cyclophosphamide-induced increase in the levels of the oxidant parameter malondialdehyde (MDA), proinflammatory nuclear factor kappa B (NF-kappaB), tumor necrosis factor alpha (TNF-alpha), and interleukin 1 beta (IL-1beta). Thiamine Pyrophosphate 9-31 interleukin 1 beta Rattus norvegicus 253-271 35156781-16 2022 RESULTS: Thiamine pyrophosphate significantly decreased the cyclophosphamide-induced increase in the levels of the oxidant parameter malondialdehyde (MDA), proinflammatory nuclear factor kappa B (NF-kappaB), tumor necrosis factor alpha (TNF-alpha), and interleukin 1 beta (IL-1beta). Thiamine Pyrophosphate 9-31 interleukin 1 alpha Rattus norvegicus 273-281 34161071-1 2021 A computational model for human transketolase was proposed, showing that thiamine diphosphate activation was based on His110 in place of His481 reported in yeast transketolase. Thiamine Pyrophosphate 73-93 transketolase Homo sapiens 32-45 34161071-1 2021 A computational model for human transketolase was proposed, showing that thiamine diphosphate activation was based on His110 in place of His481 reported in yeast transketolase. Thiamine Pyrophosphate 73-93 transketolase Homo sapiens 162-175 35370858-6 2022 This variant causes an important conformational change in the transketolase domain of OGDHL, thus reducing its binding affinity with the cofactor thiamine pyrophosphate and eventually resulting in the abnormal accumulation of glutamate in the brain. Thiamine Pyrophosphate 146-168 oxoglutarate dehydrogenase L Homo sapiens 86-91 34998824-3 2022 We showed hypoxia causes significant inhibition in TPP and free thiamin uptake by colonic NCM460 cells and by colonoid monolayers; however, it also caused a significant reduction in the expression of TPP (SLC44A4) and free thiamin (SLC19A2 and SLC19A3) transporters, and in activity of their gene promoters. Thiamine Pyrophosphate 200-203 solute carrier family 19 member 2 Homo sapiens 232-239 34998824-3 2022 We showed hypoxia causes significant inhibition in TPP and free thiamin uptake by colonic NCM460 cells and by colonoid monolayers; however, it also caused a significant reduction in the expression of TPP (SLC44A4) and free thiamin (SLC19A2 and SLC19A3) transporters, and in activity of their gene promoters. Thiamine Pyrophosphate 200-203 solute carrier family 19 member 3 Homo sapiens 244-251 34998824-5 2022 Knocking down HIF-1alpha using gene-specific siRNAs in NCM460 cells maintained under hypoxic conditions, on the other hand, led to a significant reversal in the inhibitory effect of hypoxia on TPP and free thiamin uptake, as well as on the expression of their transporters. Thiamine Pyrophosphate 193-196 hypoxia inducible factor 1 subunit alpha Homo sapiens 14-24 34998824-7 2022 In summary, hypoxia causes severe inhibition in colonic TPP and free thiamin uptake that is mediated at least in part via HIF-1alpha-mediated transcriptional mechanisms affecting their respective transporters. Thiamine Pyrophosphate 56-59 hypoxia inducible factor 1 subunit alpha Homo sapiens 122-132 2675860-3 1989 TPP is a cofactor for the pyruvate dehydrogenase complex (PDHC), alpha-ketoglutarate dehydrogenase (alpha KGDH) and transketolase (TK), three enzymes involved in cerebral glucose and energy metabolism. Thiamine Pyrophosphate 0-3 transketolase Homo sapiens 116-129 2799573-4 1989 The transketolase response to thiamine pyrophosphate (TPP effect) suggested thiamine deficiency in 32.4%, of whom 13.2% were classified as severely deficient. Thiamine Pyrophosphate 30-52 transketolase Homo sapiens 4-17 2551298-1 1989 Adenylate kinase isozyme 1 (AK1) catalyzes thiamin triphosphate (TTP) formation from thiamin diphosphate (TDP) and ADP. Thiamine Pyrophosphate 85-104 adenylate kinase 1 Homo sapiens 0-26 2551298-1 1989 Adenylate kinase isozyme 1 (AK1) catalyzes thiamin triphosphate (TTP) formation from thiamin diphosphate (TDP) and ADP. Thiamine Pyrophosphate 85-104 adenylate kinase 1 Homo sapiens 28-31 2675860-3 1989 TPP is a cofactor for the pyruvate dehydrogenase complex (PDHC), alpha-ketoglutarate dehydrogenase (alpha KGDH) and transketolase (TK), three enzymes involved in cerebral glucose and energy metabolism. Thiamine Pyrophosphate 0-3 transketolase Homo sapiens 131-133 3129964-3 1988 Transketolase concentration correlated positively with the enzyme activity both with and without in vitro addition of thiamin pyrophosphate. Thiamine Pyrophosphate 118-139 transketolase Homo sapiens 0-13 2722424-2 1989 The methods of erythrocyte transketolase activity and HPLC determination of thiamin pyrophosphate (TPP) for thiamin and erythrocyte glutathione reductase and HPLC determination of flavin adenine dinucleotide (FAD) for riboflavin were used. Thiamine Pyrophosphate 99-102 glutathione-disulfide reductase Homo sapiens 132-153 3248678-0 1988 Studies on the nature of thiamine pyrophosphate binding and dependency on divalent cations of transketolase from human erythrocytes. Thiamine Pyrophosphate 25-47 transketolase Homo sapiens 94-107 3674888-3 1987 Thiamine pyrophosphate, the coenzyme for the pyruvate dehydrogenase (PDH) component of the complex, did not inhibit ATP-dependent inactivation when used alone but it enhanced inhibition by pyruvate. Thiamine Pyrophosphate 0-22 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 45-67 3674888-3 1987 Thiamine pyrophosphate, the coenzyme for the pyruvate dehydrogenase (PDH) component of the complex, did not inhibit ATP-dependent inactivation when used alone but it enhanced inhibition by pyruvate. Thiamine Pyrophosphate 0-22 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 69-72 3674888-5 1987 A model is proposed for the pyruvate plus thiamine pyrophosphate inhibition of ATP-dependent inactivation of the pyruvate dehydrogenase complex in which pyruvate exerts its inhibition of inactivation by altering or protecting the protein substrate from phosphorylation and not by directly inhibiting PDH kinase. Thiamine Pyrophosphate 42-64 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 113-135 3674888-5 1987 A model is proposed for the pyruvate plus thiamine pyrophosphate inhibition of ATP-dependent inactivation of the pyruvate dehydrogenase complex in which pyruvate exerts its inhibition of inactivation by altering or protecting the protein substrate from phosphorylation and not by directly inhibiting PDH kinase. Thiamine Pyrophosphate 42-64 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 300-303 3439087-1 1987 Investigation of diphosphothiamine content in the mouse tissues, associated with transketolase activity in the dynamic dependence on the body provision with vitamin B1, has proved the organ-specificity of deposition and elimination of diphosphothiamine under conditions of developing thiamine deficiency. Thiamine Pyrophosphate 17-34 transketolase Mus musculus 81-94 3602224-1 1987 Erythrocyte transketolase activation by thiamin diphosphate has been studied in elderly patients with moderate or severe chronic dementia, acute alcoholic admissions and chronic alcoholics with evidence of brain damage, mostly of the Wernicke-Korsakoff type. Thiamine Pyrophosphate 40-59 transketolase Homo sapiens 12-25 3603737-1 1987 Thiamine, thiaminepyrophosphate and 4-methyl-5-beta-oxyethylthiazole are studied for their effect on the acetylcholinesterase activity in the brain, blood plasma and cells. Thiamine Pyrophosphate 10-31 acetylcholinesterase Mus musculus 105-125 3603737-3 1987 Acetylcholinesterase of the brain has been efficiently inhibited only by thiamine pyrophosphate. Thiamine Pyrophosphate 73-95 acetylcholinesterase Mus musculus 0-20 3602224-2 1987 Significantly more patients in each group than controls showed abnormal activation of transketolase, not only by 0.3 mM thiamin diphosphate (TDP) but also in further activation by increase to 3 mM. Thiamine Pyrophosphate 120-139 transketolase Homo sapiens 86-99 3558815-2 1987 This study was undertaken to delineate whether transketolase abnormality (i.e., high Michaelis Menton constant (Km) for thiamine pyrophosphate), previously reported in patients with Wernicke-Korsakoff syndrome is prevalent among familial chronic alcoholic men and their sons without prior history of alcohol abuse but who are at high risk for alcoholism. Thiamine Pyrophosphate 120-142 transketolase Homo sapiens 47-60 3558815-3 1987 Our data suggest that an inborn error (i.e., high Km of transketolase for thiamine pyrophosphate) predisposing to thiamine deficiency diseases similar to those reported in Wernicke-Korsakoff syndrome may occur in the general population. Thiamine Pyrophosphate 74-96 transketolase Homo sapiens 56-69 3762968-1 1986 We studied a thiamine-dependent enzyme, transketolase, from fibroblasts of a diabetic patient who developed Wernicke"s encephalopathy when treated with tolazamide, in order to delineate if this patient also had transketolase abnormality [high Km for thiamine pyrophosphate (TPP)], as previously reported in postalcoholic Wernicke-Korsakoff syndrome. Thiamine Pyrophosphate 250-272 transketolase Homo sapiens 40-53 3569840-4 1987 Ethanol consumption increased significantly the blood concentrations of pyruvate and lactate, decreased the activity of erythrocyte transketolase (TK), and increased the stimulation of TK activity of thiamin pyrophosphate (TPP) in lyzed blood cells (TPP effect). Thiamine Pyrophosphate 200-221 transketolase Meleagris gallopavo 185-187 3569840-4 1987 Ethanol consumption increased significantly the blood concentrations of pyruvate and lactate, decreased the activity of erythrocyte transketolase (TK), and increased the stimulation of TK activity of thiamin pyrophosphate (TPP) in lyzed blood cells (TPP effect). Thiamine Pyrophosphate 223-226 transketolase Meleagris gallopavo 185-187 3569840-4 1987 Ethanol consumption increased significantly the blood concentrations of pyruvate and lactate, decreased the activity of erythrocyte transketolase (TK), and increased the stimulation of TK activity of thiamin pyrophosphate (TPP) in lyzed blood cells (TPP effect). Thiamine Pyrophosphate 250-253 transketolase Meleagris gallopavo 185-187 3785292-4 1986 PDHa kinase activity is inhibited by ADP, thiamine pyrophosphate, and physiological levels of pyruvate and propionate. Thiamine Pyrophosphate 42-64 pyruvate dehydrogenase E1 subunit alpha 1 Homo sapiens 0-4 3711903-1 1986 The stability of rat brain transketolase, whether measured at 37 or 0 degree C, was reduced after conversion to the apo form by removal of thiamine diphosphate, as shown by a decline in the activity recovered when assayed in the presence of thiamine diphosphate. Thiamine Pyrophosphate 139-159 transketolase Rattus norvegicus 27-40 3711903-1 1986 The stability of rat brain transketolase, whether measured at 37 or 0 degree C, was reduced after conversion to the apo form by removal of thiamine diphosphate, as shown by a decline in the activity recovered when assayed in the presence of thiamine diphosphate. Thiamine Pyrophosphate 241-261 transketolase Rattus norvegicus 27-40 4090374-1 1985 Activities of the thiamine pyrophosphate-dependent enzymes (pyruvate dehydrogenase, oxoglutarate dehydrogenase, transketolase) were increased in regenerating rat liver tissue as compared with their activities in the intact tissue. Thiamine Pyrophosphate 18-40 oxoglutarate dehydrogenase Rattus norvegicus 84-110 4090374-1 1985 Activities of the thiamine pyrophosphate-dependent enzymes (pyruvate dehydrogenase, oxoglutarate dehydrogenase, transketolase) were increased in regenerating rat liver tissue as compared with their activities in the intact tissue. Thiamine Pyrophosphate 18-40 transketolase Rattus norvegicus 112-125 3426764-1 1987 Erythrocyte transketolase activation by thiamin diphosphate has been studied in alcoholic patients on admission and after treatment, which included vitamin therapy. Thiamine Pyrophosphate 40-59 transketolase Homo sapiens 12-25 3426764-2 1987 The high proportion of the patients who showed an abnormal activation of transketolase, not only by 0.3 mM thiamin diphosphate but also further activation by increasing the thiamin diphosphate to 3 mM, was reduced considerably after treatment. Thiamine Pyrophosphate 107-126 transketolase Homo sapiens 73-86 3426764-2 1987 The high proportion of the patients who showed an abnormal activation of transketolase, not only by 0.3 mM thiamin diphosphate but also further activation by increasing the thiamin diphosphate to 3 mM, was reduced considerably after treatment. Thiamine Pyrophosphate 173-192 transketolase Homo sapiens 73-86 3762968-1 1986 We studied a thiamine-dependent enzyme, transketolase, from fibroblasts of a diabetic patient who developed Wernicke"s encephalopathy when treated with tolazamide, in order to delineate if this patient also had transketolase abnormality [high Km for thiamine pyrophosphate (TPP)], as previously reported in postalcoholic Wernicke-Korsakoff syndrome. Thiamine Pyrophosphate 274-277 transketolase Homo sapiens 40-53 3762968-3 1986 We found that the above-mentioned patient and one of the diabetic kindreds with no history of Wernicke"s encephalopathy had abnormal transketolase as determined by its Km for TPP. Thiamine Pyrophosphate 175-178 transketolase Homo sapiens 133-146 4052157-3 1985 It is concluded that the failure to detect an increase in activation in the commonly used clinical test of red cell transketolase activation by raising the thiamine diphosphate concentration above about 0.3 mmol/l is likely to be due to masking of the effect of activation of the low affinity variant in haemolysates from normal red blood cells by the inhibitory effect of excess thiamine diphosphate upon the activity of the high affinity form of the enzyme with which it is mixed. Thiamine Pyrophosphate 156-176 transketolase Homo sapiens 116-129 4036754-1 1985 Transketolase (EC 2.2.1.1) was shown to be the sole enzyme protein bound to thiamine diphosphate (ThDP) as coenzyme in the soluble fraction of rat liver. Thiamine Pyrophosphate 76-96 transketolase Rattus norvegicus 0-13 4036754-1 1985 Transketolase (EC 2.2.1.1) was shown to be the sole enzyme protein bound to thiamine diphosphate (ThDP) as coenzyme in the soluble fraction of rat liver. Thiamine Pyrophosphate 98-102 transketolase Rattus norvegicus 0-13 6497956-4 1984 Not only do the apoenzymes isolated from each of the two fractions differ in the way in which they recombine with thiamine pyrophosphate but kinetic analysis of the results shows that each fraction contains at least two variants of transketolase differing in their affinity for thiamine pyrophosphate. Thiamine Pyrophosphate 114-136 transketolase Homo sapiens 232-245 6434322-6 1984 This association suggests that a variant transketolase and thiamin deficiency together contribute to the pathogenesis of the brain damage of the Wernicke-Korsakoff syndrome by some mechanism independent of apparent Km values for thiamin diphosphate. Thiamine Pyrophosphate 229-248 transketolase Homo sapiens 41-54 4074776-1 1985 An analysis of a proteolytic hydrolysate of pig liver transketolase by thin-layer chromatography revealed the presence of a coenzyme-containing material which differed from free thiamine pyrophosphate in chromatographic behaviour. Thiamine Pyrophosphate 178-200 transketolase Sus scrofa 54-67 6497956-4 1984 Not only do the apoenzymes isolated from each of the two fractions differ in the way in which they recombine with thiamine pyrophosphate but kinetic analysis of the results shows that each fraction contains at least two variants of transketolase differing in their affinity for thiamine pyrophosphate. Thiamine Pyrophosphate 278-300 transketolase Homo sapiens 232-245 6545581-3 1984 The amount of coenzyme-unsaturated apotransketolase was assessed by measuring the TPP effect--determining transketolase activity with and without the addition of TPP in vitro. Thiamine Pyrophosphate 162-165 transketolase Mus musculus 38-51 6191889-5 1983 The addition of thiamin diphosphate to the staining mixture darkened some but not all bands of transketolase activity. Thiamine Pyrophosphate 16-35 transketolase Homo sapiens 95-108 6545581-2 1984 The process of conversion of [14C]thiamin to thiamin pyrophosphate (TPP) was monitored by measuring the activities of transketolase, pyruvate dehydrogenase, and oxoglutarate dehydrogenase. Thiamine Pyrophosphate 45-66 transketolase Mus musculus 118-131 6545581-2 1984 The process of conversion of [14C]thiamin to thiamin pyrophosphate (TPP) was monitored by measuring the activities of transketolase, pyruvate dehydrogenase, and oxoglutarate dehydrogenase. Thiamine Pyrophosphate 68-71 transketolase Mus musculus 118-131 6545581-3 1984 The amount of coenzyme-unsaturated apotransketolase was assessed by measuring the TPP effect--determining transketolase activity with and without the addition of TPP in vitro. Thiamine Pyrophosphate 82-85 transketolase Mus musculus 38-51 6649520-1 1983 In blood of patients with cancer of stomach and mammary gland total thiamin content was measured by means of fluorimetric procedure, thiamindiphosphate (TDP)--by an enzymatic method, activity of transketolase was estimated in presence or in absence of TDP. Thiamine Pyrophosphate 133-151 transketolase Homo sapiens 195-208 6649520-2 1983 Concentration of total thiamin in blood of healthy persons was 7.65 +/- 0.40 micrograms/100 ml; thiamindiphosphate--5.06 micrograms/100 ml activity of transketolase was 11.59 +/- 0.23 mmol/l. Thiamine Pyrophosphate 96-114 transketolase Homo sapiens 151-164 6191889-7 1983 This heterogeneity might need recognition when thiamin nutritional sufficiency is assessed by the "thiamin diphosphate effect" on erythrocyte transketolase. Thiamine Pyrophosphate 99-118 transketolase Homo sapiens 142-155 7113098-1 1982 A modified method is described for determination of transketolase (TK) activity in the blood and the degree of its stimulation under the effect of exogenous thiamine diphosphate (TDP-effect). Thiamine Pyrophosphate 157-177 transketolase Homo sapiens 52-65 6139882-14 1983 Finally focus is set on the recent suggestion that the syndrome may in some cases result from an inborn enzymatic abnormality comprising low binding of thiamine pyrophosphate to transketolase. Thiamine Pyrophosphate 152-174 transketolase Homo sapiens 178-191 6139240-1 1983 Nitrofurazone, given orally at doses of 10 and 20 mg/kg for seven days, decreased the activity of erythrocyte transketolase (TK) and increased the activation of TK by thiamin pyrophosphate (TPP effect %). Thiamine Pyrophosphate 167-188 transketolase like 1 Gallus gallus 161-163 7113098-1 1982 A modified method is described for determination of transketolase (TK) activity in the blood and the degree of its stimulation under the effect of exogenous thiamine diphosphate (TDP-effect). Thiamine Pyrophosphate 157-177 transketolase Homo sapiens 67-69 6115895-6 1981 Red blood cells from this group of kittens also showed a transitory incomplete saturation of transketolase with thiamin pyrophosphate. Thiamine Pyrophosphate 112-133 transketolase Homo sapiens 93-106 7223515-1 1981 The thiamine contents, transketolase activity and "thiamine diphosphate effect" (TDP effect) of the transketolase activity were measured in the blood of alcoholic patients during withdrawal, before and after thiamine administration (50 mg) for 10 days. Thiamine Pyrophosphate 51-71 transketolase Homo sapiens 100-113 7270482-1 1981 A semiautomated method is described which uses the Abbott ABA-100 bichromatic analyzer to measure the stimulation of erythrocyte transketolase by thiamin pyrophosphate (the thiamin pyrophosphate effect). Thiamine Pyrophosphate 146-167 transketolase Homo sapiens 129-142 7270482-1 1981 A semiautomated method is described which uses the Abbott ABA-100 bichromatic analyzer to measure the stimulation of erythrocyte transketolase by thiamin pyrophosphate (the thiamin pyrophosphate effect). Thiamine Pyrophosphate 173-194 transketolase Homo sapiens 129-142 7097279-3 1982 However, thiamine pyrophosphate remained tightly bound to transketolase in homogenates in which it dissociated completely from another thiamine pyrophosphate-dependent enzyme, the pyruvate dehydrogenase complex. Thiamine Pyrophosphate 9-31 transketolase Mus musculus 58-71 7097279-3 1982 However, thiamine pyrophosphate remained tightly bound to transketolase in homogenates in which it dissociated completely from another thiamine pyrophosphate-dependent enzyme, the pyruvate dehydrogenase complex. Thiamine Pyrophosphate 135-157 transketolase Mus musculus 58-71 7248378-7 1981 It was assumed that in vivo the subunits of rat liver transketolase do not exist in a free state and their association into dimers occurs immediately after completion of the polypeptide chain biosynthesis in the presence of thiamine pyrophosphate. Thiamine Pyrophosphate 224-246 transketolase Rattus norvegicus 54-67 6256098-3 1980 The values of the transketolase activity of the two groups were statistically correlated with the levels of the thiamine pyrophosphate effect and the vitamin B1 content of the blood. Thiamine Pyrophosphate 112-134 transketolase Homo sapiens 18-31 7388070-3 1980 The data obtained suggest that pig liver transketolase contains covalently bound thiamine pyrophosphate. Thiamine Pyrophosphate 81-103 transketolase Sus scrofa 41-54 7448192-6 1980 Transketolase contains about 2 mol of thiamine pyrophosphate per mol of protein and does not require metal ions for its catalytic activity. Thiamine Pyrophosphate 38-60 transketolase Sus scrofa 0-13 6110711-8 1981 In both cases we obtained a more (20-40% in blood) or less (5-10% in liver) pronounced TPP-stimulatory effect of transketolase activity. Thiamine Pyrophosphate 87-90 transketolase Mus musculus 113-126 7405142-7 1980 The results obtained indicate high transketolase saturation with thiamine diphosphate with low thiamine concentration in blood. Thiamine Pyrophosphate 65-85 transketolase Homo sapiens 35-48 453718-2 1979 This intermediate product of glucose metabolism is the result of transketolase activity for which thiamine pyrophosphate is one of the co-factors. Thiamine Pyrophosphate 98-120 transketolase Homo sapiens 65-78 7438971-5 1980 In the second case, red cell transketolase indicated thiamine pyrophosphate deficiency. Thiamine Pyrophosphate 53-75 transketolase Homo sapiens 29-42 228770-3 1979 The anticoenzymic effect of hydroxythiamine pyrophosphate with respect to transketolase is not observed in vivo at maximal concentration of the anticoenzyme in tissues due to the absence of competitive interactions with thiamine pyrophosphate. Thiamine Pyrophosphate 35-57 transketolase Homo sapiens 74-87 927453-2 1977 Transketolase in fibroblasts from the patients with the syndrome bound thiamine pyrophosphate less avidly than control lines. Thiamine Pyrophosphate 71-93 transketolase Homo sapiens 0-13 664470-4 1978 Endogenous thiamin diphosphate (about 1/3 of the initial content), resistent to dialysis and gel filtration, was apparently included in transketolase in hyaloplasmic fraction of rat liver tissue. Thiamine Pyrophosphate 11-30 transketolase Rattus norvegicus 136-149 627916-0 1978 The determination of thiamin pyrophosphate in blood and other tissues, and its correlation with erythrocyte transketolase activity. Thiamine Pyrophosphate 21-42 transketolase Homo sapiens 108-121 41031-6 1979 All the activity of pig liver transketolase was lost upon incubation at pH 5.0 for two hours even at 0 degrees C but about 40% of the original activity could be restored by the addition of excess thiamine pyrophosphate and CaCl2. Thiamine Pyrophosphate 196-218 transketolase Sus scrofa 30-43 927453-3 1977 The apparent Km for thiamine pyrophosphate was 195 +/- 31 micron for transketolase in extracts of the patients" cells as compared to 16 +/- 2 micron in six control lines (means +/- S.E.M. Thiamine Pyrophosphate 20-42 transketolase Homo sapiens 69-82 183746-7 1976 Initial-velocity patterns of the overall pyruvate dehydrogenase reaction obtained with varying TPP, CoA and NAD+ concentrations at a fixed pyruvate concentration were consistent with a sequential three-site Ping Pong mechanism; in the presence of oxaloacetate and citrate synthase to remove acetyl-CoA (an inhibitor of the overall reaction) the values of Km for NAD+ and CoA were 53+/- 5 muM and 1.9+/-0.2 muM respectively. Thiamine Pyrophosphate 95-98 citrate synthase Sus scrofa 264-280 1253408-1 1976 We describe optimized, ultraviolet spectrophotometric procedures for determination of erythrocyte transketolase, glutathione reductase, and aspartate aminotransferase activity, and their activation by their respective coenzymes--thiamine pyrophosphate, flavin-adenine dinucleotide, and pyridoxal-5-phosphate--as tests for vitamin B1, B2, and B6 deficiency. Thiamine Pyrophosphate 229-251 transketolase Homo sapiens 98-111 982621-0 1976 [Effect of C-4"-modification of thiamine pyrophosphate on its coenzyme activity in the oxidative decarboxylation of pyruvic acid reaction]. Thiamine Pyrophosphate 32-54 complement C4A (Rodgers blood group) Homo sapiens 11-14 982621-1 1976 Interaction was studied between pyruvate dehydrogenase (EC 1.2.4.1) and C-4"-substituted analogs of thiaminpyrophosphate, 4"-N (CH3)-TPP, 4"-N(CH3)2-TPP and OH-TPP. Thiamine Pyrophosphate 100-120 complement C4A (Rodgers blood group) Homo sapiens 72-75 1253408-1 1976 We describe optimized, ultraviolet spectrophotometric procedures for determination of erythrocyte transketolase, glutathione reductase, and aspartate aminotransferase activity, and their activation by their respective coenzymes--thiamine pyrophosphate, flavin-adenine dinucleotide, and pyridoxal-5-phosphate--as tests for vitamin B1, B2, and B6 deficiency. Thiamine Pyrophosphate 229-251 glutathione-disulfide reductase Homo sapiens 113-134 33759569-4 2021 Considerable amount of the microbiota-generated vitamin B1 exists in the form of TPP, and colonocytes can efficiently absorb this TPP via a high-affinity and specific carrier-mediated mechanism that involves the recently cloned colonic TPP transporter (cTPPT; product of SLC44A4 gene). Thiamine Pyrophosphate 130-133 solute carrier family 44 member 4 Homo sapiens 271-278 1262137-4 1976 The thiamine status was measured by determining the thiamine pyrophosphate stimulating effect of transketolase enzyme activity in whole blood. Thiamine Pyrophosphate 52-74 transketolase Homo sapiens 97-110 1093550-0 1975 The binding of thiamine pyrophosphate with transketolase in equilibrium conditions. Thiamine Pyrophosphate 15-37 transketolase Homo sapiens 43-56 5096513-5 1971 The specific effect on transketolase by uremic material was established by showing suppressed formation of S7P from R5P also in the presence of excess cofactor thiamine pyrophosphate and of the other substrate xylulose-5-phosphate. Thiamine Pyrophosphate 160-182 transketolase Homo sapiens 23-36 5820638-10 1969 Incubation of oxoglutarate dehydrogenase with radioactive glyoxylate produced radioactive carbon dioxide; radioactivity was also recovered in the portion of the enzyme identified with thiamin pyrophosphate. Thiamine Pyrophosphate 184-205 oxoglutarate dehydrogenase Homo sapiens 14-40 20247663-0 1947 The effect of insulin on the concentration of diphosphothiamine in the blood. Thiamine Pyrophosphate 46-63 insulin Homo sapiens 14-21 4564138-0 1972 [Interaction of thiamine pyrophosphate and transketolase]. Thiamine Pyrophosphate 16-38 transketolase Homo sapiens 43-56 33217405-2 2021 In a recent paper, we showed the difference between the first stage of the one-substrate and the two-substrate transketolase reactions - the possibility of transfer of glycolaldehyde formed as a result of cleavage of the donor substrate from the thiazole ring of thiamine diphosphate to its aminopyrimidine ring through the tricycle formation stage, which is necessary for binding and splitting the second molecule of donor substrate [O.N. Thiamine Pyrophosphate 263-283 transketolase Homo sapiens 111-124 33868388-3 2021 A major natural thiamine derivative, thiamine diphosphate (ThDP), is a coenzyme of central metabolism, also known to affect transcriptional activity of the master metabolic regulator and genome guardian p53. Thiamine Pyrophosphate 37-57 tumor protein p53 Homo sapiens 203-206 33868388-3 2021 A major natural thiamine derivative, thiamine diphosphate (ThDP), is a coenzyme of central metabolism, also known to affect transcriptional activity of the master metabolic regulator and genome guardian p53. Thiamine Pyrophosphate 59-63 tumor protein p53 Homo sapiens 203-206 33868388-10 2021 Correlation analysis of the p53 expression and enzymatic activities upon variations in cellular thiamine/ThDP levels indicates that p21 knockdown substitutes positive correlation of the p53 expression with the activity of 2-oxoglutarate dehydrogenase complex (OGDHC) for that with the activity of glutamate dehydrogenase (GDH). Thiamine Pyrophosphate 105-109 tumor protein p53 Homo sapiens 28-31 33868388-10 2021 Correlation analysis of the p53 expression and enzymatic activities upon variations in cellular thiamine/ThDP levels indicates that p21 knockdown substitutes positive correlation of the p53 expression with the activity of 2-oxoglutarate dehydrogenase complex (OGDHC) for that with the activity of glutamate dehydrogenase (GDH). Thiamine Pyrophosphate 105-109 H3 histone pseudogene 16 Homo sapiens 132-135 33868388-10 2021 Correlation analysis of the p53 expression and enzymatic activities upon variations in cellular thiamine/ThDP levels indicates that p21 knockdown substitutes positive correlation of the p53 expression with the activity of 2-oxoglutarate dehydrogenase complex (OGDHC) for that with the activity of glutamate dehydrogenase (GDH). Thiamine Pyrophosphate 105-109 tumor protein p53 Homo sapiens 186-189 33868388-10 2021 Correlation analysis of the p53 expression and enzymatic activities upon variations in cellular thiamine/ThDP levels indicates that p21 knockdown substitutes positive correlation of the p53 expression with the activity of 2-oxoglutarate dehydrogenase complex (OGDHC) for that with the activity of glutamate dehydrogenase (GDH). Thiamine Pyrophosphate 105-109 glutamate dehydrogenase 1 Homo sapiens 297-320 33868388-10 2021 Correlation analysis of the p53 expression and enzymatic activities upon variations in cellular thiamine/ThDP levels indicates that p21 knockdown substitutes positive correlation of the p53 expression with the activity of 2-oxoglutarate dehydrogenase complex (OGDHC) for that with the activity of glutamate dehydrogenase (GDH). Thiamine Pyrophosphate 105-109 glutamate dehydrogenase 1 Homo sapiens 261-264 33217405-6 2021 Therefore, a significant decrease in the reaction rate of the one-substrate transketolase reaction compared to the two-substrate reaction is due to the stage of transferring the first glycolaldehyde molecule from the thiazole ring to the aminopyrimidine ring of thiamine diphosphate. Thiamine Pyrophosphate 262-282 transketolase Homo sapiens 76-89 33354793-4 2021 Thiamine diphosphate is a cofactor for transketolase, including erythrocyte transketolase (ETK). Thiamine Pyrophosphate 0-20 transketolase Homo sapiens 39-52 33040724-9 2020 The activity of glutamate dehydrogenase GDH2 isoenzyme strongly decreased, while the activities of NADP+-dependent isocitrate dehydrogenase (IDH) and malic enzymes, as well as the activity of 2-oxoglutarate dehydrogenase complex at its endogenous ThDP level, were elevated. Thiamine Pyrophosphate 247-251 glutamate dehydrogenase 2 Homo sapiens 40-44 33119470-12 2021 Mitochondrial fission in TPP-treated heart was also inhibited, which was confirmed by a decrease in the phosphorylation level of DRP1. Thiamine Pyrophosphate 25-28 collapsin response mediator protein 1 Rattus norvegicus 129-133 32404369-3 2020 Among these enzymes, thiamine monophosphate kinase (ThiL) catalyzes the final step of this pathway, phosphorylating thiamine monophosphate to produce TPP. Thiamine Pyrophosphate 150-153 acetyl-CoA acetyltransferase 1 Homo sapiens 52-56 32404369-5 2020 We demonstrate that thiL deletion abolishes not only thiamine biosynthesis but also thiamine salvage capability and results in growth defects of the DeltathiL strain even in the presence of thiamine derivatives, except for TPP. Thiamine Pyrophosphate 223-226 acetyl-CoA acetyltransferase 1 Homo sapiens 20-24 32695416-3 2020 Here, the 1.9 A resolution crystal structure of human DHTKD1 is solved in complex with the thi-amine diphosphate co-factor. Thiamine Pyrophosphate 91-112 dehydrogenase E1 and transketolase domain containing 1 Homo sapiens 54-60 33040724-3 2020 Thiamine diphosphate (ThDP), which is a major thiamine derivative, affects p53 binding to DNA. Thiamine Pyrophosphate 0-20 tumor protein p53 Homo sapiens 75-78 33040724-3 2020 Thiamine diphosphate (ThDP), which is a major thiamine derivative, affects p53 binding to DNA. Thiamine Pyrophosphate 22-26 tumor protein p53 Homo sapiens 75-78 32079473-3 2020 This RS is located in the 5 untranslated region upstream of thiC gene, encoding a protein involved in TPP biosynthesis, an essential cofactor for all living beings. Thiamine Pyrophosphate 103-106 phosphomethylpyrimidine synthase ThiC Neisseria meningitidis MC58 61-65 32079473-5 2020 Northern blot analysis of thiC mRNA and reporter gene studies confirmed the presence of an active TPP-sensing RS. Thiamine Pyrophosphate 98-101 phosphomethylpyrimidine synthase ThiC Neisseria meningitidis MC58 26-30 30880633-4 2019 Using this approach, we identified the tptA gene in A. fumigatus, which shares homology with the Saccharomyces cerevisiae thiamine pyrophosphate (ThPP) transporter encoding gene tpc1. Thiamine Pyrophosphate 122-144 thiamine transporter TPC1 Saccharomyces cerevisiae S288C 178-182 32466567-2 2020 Thiamine diphosphate (ThDP, cocarboxylase) is an essential activator of the enzyme and inhibits p53-DNA binding in cancer cells. Thiamine Pyrophosphate 0-20 tumor protein p53 Homo sapiens 96-99 32466567-2 2020 Thiamine diphosphate (ThDP, cocarboxylase) is an essential activator of the enzyme and inhibits p53-DNA binding in cancer cells. Thiamine Pyrophosphate 22-26 tumor protein p53 Homo sapiens 96-99 32466567-5 2020 Molecular mechanisms of ThDP action on cellular viability and their interplay with the cisplatin and p53-p21 pathways are characterized. Thiamine Pyrophosphate 24-28 tumor protein p53 Homo sapiens 101-104 32466567-5 2020 Molecular mechanisms of ThDP action on cellular viability and their interplay with the cisplatin and p53-p21 pathways are characterized. Thiamine Pyrophosphate 24-28 H3 histone pseudogene 16 Homo sapiens 105-108 32466567-10 2020 Cellular levels of the catalytically competent ThDP OGDHC holoenzyme are dysregulated by p21 knockdown and correlate negatively with the A549 viability. Thiamine Pyrophosphate 47-51 H3 histone pseudogene 16 Homo sapiens 89-92 32466567-12 2020 The similarity, non-additivity, and p21 dependence of the dual actions of ThDP and cisplatin on A549 cells manifest a common OGDHC-mediated mechanism of the viability decrease. Thiamine Pyrophosphate 74-78 H3 histone pseudogene 16 Homo sapiens 36-39 32466567-13 2020 High ThDP saturation of OGDHC compromises the redox state of A549 cells under the control of p53-p21 axes. Thiamine Pyrophosphate 5-9 tumor protein p53 Homo sapiens 93-96 32466567-13 2020 High ThDP saturation of OGDHC compromises the redox state of A549 cells under the control of p53-p21 axes. Thiamine Pyrophosphate 5-9 H3 histone pseudogene 16 Homo sapiens 97-100 32139871-1 2020 We recently characterised a low-activity form of E. coli transketolase, TKlow, which also binds the cofactor thiamine pyrophosphate (TPP) with an affinity up to two-orders of magnitude lower than the previously known high TPP-affinity and high-activity form, TKhigh, in the presence of Mg2+. Thiamine Pyrophosphate 109-131 transketolase Homo sapiens 57-70 32139871-1 2020 We recently characterised a low-activity form of E. coli transketolase, TKlow, which also binds the cofactor thiamine pyrophosphate (TPP) with an affinity up to two-orders of magnitude lower than the previously known high TPP-affinity and high-activity form, TKhigh, in the presence of Mg2+. Thiamine Pyrophosphate 133-136 transketolase Homo sapiens 57-70 32139871-1 2020 We recently characterised a low-activity form of E. coli transketolase, TKlow, which also binds the cofactor thiamine pyrophosphate (TPP) with an affinity up to two-orders of magnitude lower than the previously known high TPP-affinity and high-activity form, TKhigh, in the presence of Mg2+. Thiamine Pyrophosphate 222-225 transketolase Homo sapiens 57-70 32139871-4 2020 Transketolase HPA affinity again revealed the two distinct forms of transketolase at a TKhigh:TKlow ratio that matched those observed previously via TPP binding to each variant. Thiamine Pyrophosphate 149-152 transketolase Homo sapiens 68-81 30946572-2 2019 Two hypotheses for possible pathways involving a thiamine diphosphate-dependent cleavage, either C-C cleavage of a ketol or diketone aryl C3 substrate or decarboxylation of an aryl C2 substrate, were investigated by expression and purification of the recombinant enzymes and expression of dehydrogenase and oxidase enzymes also found in the gene clusters. Thiamine Pyrophosphate 49-69 Gfo/Idh/MocA family oxidoreductase Rhodococcus jostii RHA1 289-314 30880633-4 2019 Using this approach, we identified the tptA gene in A. fumigatus, which shares homology with the Saccharomyces cerevisiae thiamine pyrophosphate (ThPP) transporter encoding gene tpc1. Thiamine Pyrophosphate 146-150 thiamine transporter TPC1 Saccharomyces cerevisiae S288C 178-182 30880633-5 2019 Heterologous expression of tpc1 in the tptA deletion mutant completely restored the ThPP auxotrophy phenotype, suggesting that Tpc1 and TptA are functional orthologues. Thiamine Pyrophosphate 84-88 thiamine transporter TPC1 Saccharomyces cerevisiae S288C 27-31 31639155-1 2019 Colonocytes possess a specific carrier-mediated uptake process for the microbiota-generated thiamin (vitamin B1) pyrophosphate (TPP) that involves the TPP transporter (TPPT; product of the SLC44A4 gene). Thiamine Pyrophosphate 128-131 solute carrier family 44 member 4 Homo sapiens 151-166 31617701-6 2019 Successful exchange of Cu+ for Ga3+ is only possible when GaCl3 is complexed with either TPP or DPP. Thiamine Pyrophosphate 89-92 succinyl-CoA:glutarate-CoA transferase Homo sapiens 31-34 31695144-1 2019 Transketolase (TK) cofactor binding has been studied extensively over many years, yet certain mysteries remain, such as a lack of consensus on the cooperativity of thiamine pyrophosphate (TPP) binding into the two active sites, in the presence and absence of the divalent cation, Mg2+. Thiamine Pyrophosphate 164-186 transketolase Homo sapiens 0-13 31695144-1 2019 Transketolase (TK) cofactor binding has been studied extensively over many years, yet certain mysteries remain, such as a lack of consensus on the cooperativity of thiamine pyrophosphate (TPP) binding into the two active sites, in the presence and absence of the divalent cation, Mg2+. Thiamine Pyrophosphate 188-191 transketolase Homo sapiens 0-13 31639155-1 2019 Colonocytes possess a specific carrier-mediated uptake process for the microbiota-generated thiamin (vitamin B1) pyrophosphate (TPP) that involves the TPP transporter (TPPT; product of the SLC44A4 gene). Thiamine Pyrophosphate 128-131 solute carrier family 44 member 4 Homo sapiens 168-172 31639155-1 2019 Colonocytes possess a specific carrier-mediated uptake process for the microbiota-generated thiamin (vitamin B1) pyrophosphate (TPP) that involves the TPP transporter (TPPT; product of the SLC44A4 gene). Thiamine Pyrophosphate 128-131 solute carrier family 44 member 4 Homo sapiens 189-196 31639155-7 2019 The inhibitory effect of EHEC on TPP uptake by NCM460 was found to be associated with reduction in the rate of transcription of the SLC44A4 gene as indicated by the significant reduction in the activity of the SLC44A4 promoter transfected into EHEC infected cells. Thiamine Pyrophosphate 33-36 solute carrier family 44 member 4 Homo sapiens 132-139 31639155-7 2019 The inhibitory effect of EHEC on TPP uptake by NCM460 was found to be associated with reduction in the rate of transcription of the SLC44A4 gene as indicated by the significant reduction in the activity of the SLC44A4 promoter transfected into EHEC infected cells. Thiamine Pyrophosphate 33-36 solute carrier family 44 member 4 Homo sapiens 210-217 31639155-9 2019 Finally, blocking the ERK1/2 and NF-kB signaling pathways in NCM460 cells significantly reversed the level of EHEC inhibition in TPP uptake and TPPT expression. Thiamine Pyrophosphate 129-132 mitogen-activated protein kinase 3 Homo sapiens 22-28 31639155-10 2019 Collectively, these findings show, for the first time, that EHEC infection significantly inhibit colonic uptake of TPP, and that this effect appears to be exerted at the level of SLC44A4 transcription and involves the ERK1/2 and NF-kB signaling pathways. Thiamine Pyrophosphate 115-118 solute carrier family 44 member 4 Homo sapiens 179-186 31639155-10 2019 Collectively, these findings show, for the first time, that EHEC infection significantly inhibit colonic uptake of TPP, and that this effect appears to be exerted at the level of SLC44A4 transcription and involves the ERK1/2 and NF-kB signaling pathways. Thiamine Pyrophosphate 115-118 mitogen-activated protein kinase 3 Homo sapiens 218-224 31288420-0 2019 Utility of Whole Blood Thiamine Pyrophosphate Evaluation in TPK1-Related Diseases. Thiamine Pyrophosphate 23-45 thiamin pyrophosphokinase 1 Homo sapiens 60-64 31295743-0 2019 Bilateral Striatal Necrosis with Polyneuropathy with a Novel SLC25A19 (Mitochondrial Thiamine Pyrophosphate Carrier OMIMI*606521) Mutation: Treatable Thiamine Metabolic Disorder-A Report of Two Indian Cases. Thiamine Pyrophosphate 85-107 solute carrier family 25 member 19 Homo sapiens 61-69 31415630-3 2019 Transketolase (TKT) is a thiamine pyrophosphate (vitamin B1)-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway by feeding excess sugar phosphates into the main carbohydrate metabolic pathways to generate biosynthetic reducing capacity in the form of NADPH as a substrate for ROS generation. Thiamine Pyrophosphate 25-47 transketolase Homo sapiens 0-13 31415630-3 2019 Transketolase (TKT) is a thiamine pyrophosphate (vitamin B1)-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway by feeding excess sugar phosphates into the main carbohydrate metabolic pathways to generate biosynthetic reducing capacity in the form of NADPH as a substrate for ROS generation. Thiamine Pyrophosphate 25-47 transketolase Homo sapiens 15-18 31288420-3 2019 We report a case of TPK1-related disease presenting with Leigh-like syndrome and review the diagnostic utility of thiamine pyrophosphate (TPP) blood measurement. Thiamine Pyrophosphate 114-136 thiamin pyrophosphokinase 1 Homo sapiens 20-24 31288420-3 2019 We report a case of TPK1-related disease presenting with Leigh-like syndrome and review the diagnostic utility of thiamine pyrophosphate (TPP) blood measurement. Thiamine Pyrophosphate 138-141 thiamin pyrophosphokinase 1 Homo sapiens 20-24 31288420-13 2019 Blood TPP measurement represents a fast and cost-effective diagnostic tool in TPK1-related diseases. Thiamine Pyrophosphate 6-9 thiamin pyrophosphokinase 1 Homo sapiens 78-82 31095747-5 2019 TPK1 patients show decreased concentrations of thiamine pyrophosphate in blood and muscle. Thiamine Pyrophosphate 47-69 thiamin pyrophosphokinase 1 Homo sapiens 0-4 31244575-7 2019 A 2-step enzymatic cascade consisting of a thiamine diphosphate (ThDP)-dependent carboligase and an alcohol dehydrogenase is presented here as a model reaction. Thiamine Pyrophosphate 43-63 aldo-keto reductase family 1 member A1 Homo sapiens 100-121 31244575-7 2019 A 2-step enzymatic cascade consisting of a thiamine diphosphate (ThDP)-dependent carboligase and an alcohol dehydrogenase is presented here as a model reaction. Thiamine Pyrophosphate 65-69 aldo-keto reductase family 1 member A1 Homo sapiens 100-121 30950790-3 2019 In the current report, we describe biochemical and genetic data demonstrating that thiS motif RNAs function as sensors of the thiamin precursor HMP-PP, which is fused with HET-P ultimately to form the final active coenzyme thiamin pyrophosphate (TPP). Thiamine Pyrophosphate 223-244 inner membrane mitochondrial protein Homo sapiens 144-147 30950790-3 2019 In the current report, we describe biochemical and genetic data demonstrating that thiS motif RNAs function as sensors of the thiamin precursor HMP-PP, which is fused with HET-P ultimately to form the final active coenzyme thiamin pyrophosphate (TPP). Thiamine Pyrophosphate 246-249 inner membrane mitochondrial protein Homo sapiens 144-147 30867460-1 2019 Thiamine monophosphate kinase (ThiL) catalyzes the last step of thiamine pyrophosphate (TPP) synthesis, the ATP-dependent phosphorylation of thiamine monophosphate (TMP) to thiamine pyrophosphate. Thiamine Pyrophosphate 64-86 acetyl-CoA acetyltransferase 1 Homo sapiens 31-35 30867460-1 2019 Thiamine monophosphate kinase (ThiL) catalyzes the last step of thiamine pyrophosphate (TPP) synthesis, the ATP-dependent phosphorylation of thiamine monophosphate (TMP) to thiamine pyrophosphate. Thiamine Pyrophosphate 88-91 acetyl-CoA acetyltransferase 1 Homo sapiens 31-35 30867460-1 2019 Thiamine monophosphate kinase (ThiL) catalyzes the last step of thiamine pyrophosphate (TPP) synthesis, the ATP-dependent phosphorylation of thiamine monophosphate (TMP) to thiamine pyrophosphate. Thiamine Pyrophosphate 173-195 acetyl-CoA acetyltransferase 1 Homo sapiens 31-35 30867460-2 2019 We solved the structure of ThiL from the human pathogen A. baumanii in complex with a pair of substrates TMP and a non-hydrolyzable adenosine triphosphate analog, and in complex with a pair of products TPP and adenosine diphosphate. Thiamine Pyrophosphate 202-205 acetyl-CoA acetyltransferase 1 Homo sapiens 27-31 29332858-8 2018 Increased production of IL-1beta and MDA by cisplatin was inhibited by TPP, while it was not inhibited by thiamine. Thiamine Pyrophosphate 71-74 interleukin 1 beta Rattus norvegicus 24-32 30459934-3 2018 Upon uptake, thiamine pyrophosphokinase-1 (TPK1) facilitates the rapid phosphorylation of thiamine into thiamine pyrophosphate (TPP). Thiamine Pyrophosphate 104-126 thiamin pyrophosphokinase 1 Homo sapiens 13-41 30459934-3 2018 Upon uptake, thiamine pyrophosphokinase-1 (TPK1) facilitates the rapid phosphorylation of thiamine into thiamine pyrophosphate (TPP). Thiamine Pyrophosphate 104-126 thiamin pyrophosphokinase 1 Homo sapiens 43-47 30459934-3 2018 Upon uptake, thiamine pyrophosphokinase-1 (TPK1) facilitates the rapid phosphorylation of thiamine into thiamine pyrophosphate (TPP). Thiamine Pyrophosphate 128-131 thiamin pyrophosphokinase 1 Homo sapiens 13-41 30459934-3 2018 Upon uptake, thiamine pyrophosphokinase-1 (TPK1) facilitates the rapid phosphorylation of thiamine into thiamine pyrophosphate (TPP). Thiamine Pyrophosphate 128-131 thiamin pyrophosphokinase 1 Homo sapiens 43-47 30224059-0 2018 Ca2+-dependent inhibition of branched-chain alpha-ketoacid dehydrogenase kinase by thiamine pyrophosphate. Thiamine Pyrophosphate 83-105 branched chain keto acid dehydrogenase kinase Homo sapiens 29-79 30224059-2 2018 Thiamine pyrophosphate (TPP) is required as a coenzyme for the E1 component of the BCKDH complex and can also bring about activation of the complex by inhibiting BDK. Thiamine Pyrophosphate 0-22 branched chain keto acid dehydrogenase kinase Homo sapiens 162-165 30224059-2 2018 Thiamine pyrophosphate (TPP) is required as a coenzyme for the E1 component of the BCKDH complex and can also bring about activation of the complex by inhibiting BDK. Thiamine Pyrophosphate 24-27 branched chain keto acid dehydrogenase kinase Homo sapiens 162-165 30062480-0 2018 Extended substrate range of thiamine diphosphate-dependent MenD enzyme by coupling of two C-C-bonding reactions. Thiamine Pyrophosphate 28-48 EBP cholestenol delta-isomerase Homo sapiens 59-63 30224059-3 2018 The present study shows that free Ca2+ in the physiological range greatly increases the sensitivity of BDK to inhibition by TPP (IC50 of 2.5 muM in the presence of 1 muM free Ca2+). Thiamine Pyrophosphate 124-127 branched chain keto acid dehydrogenase kinase Homo sapiens 103-106 30224059-3 2018 The present study shows that free Ca2+ in the physiological range greatly increases the sensitivity of BDK to inhibition by TPP (IC50 of 2.5 muM in the presence of 1 muM free Ca2+). Thiamine Pyrophosphate 124-127 latexin Homo sapiens 141-144 30224059-3 2018 The present study shows that free Ca2+ in the physiological range greatly increases the sensitivity of BDK to inhibition by TPP (IC50 of 2.5 muM in the presence of 1 muM free Ca2+). Thiamine Pyrophosphate 124-127 latexin Homo sapiens 166-169 28950391-3 2018 We hypothesized that protective PPP action in diabetes and eventually even more severely in concomitant DKD might be compromised by limited intracellular availability of an active TKT cofactor thiamine diphosphate (TDP). Thiamine Pyrophosphate 193-213 transketolase Homo sapiens 180-183 28931541-2 2017 We have previously shown that human colonocytes possess an efficient carrier-mediated uptake process for TPP that involves the SLC44A4 system and this uptake process is adaptively regulated by prevailing extracellular TPP level. Thiamine Pyrophosphate 105-108 solute carrier family 44 member 4 Homo sapiens 127-134 29440497-8 2018 Kinetic analysis shows that PS inhibits AHAS by a combination of events involving FAD oxidation and chemical alteration of ThDP. Thiamine Pyrophosphate 123-127 chlorsulfuron/imidazolinone resistant 1 Arabidopsis thaliana 40-44 29405997-6 2018 Thiamine diphosphate dependent MenD proved to be quite a robust enzyme; nevertheless, enzyme operational stability decay occurs in the reactor. Thiamine Pyrophosphate 0-20 EBP cholestenol delta-isomerase Homo sapiens 31-35 29191460-1 2018 Herein are reported unique properties of the novel human thiamin diphosphate (ThDP)-dependent enzyme 2-oxoadipate dehydrogenase (hE1a), known as dehydrogenase E1 and transketolase domain-containing protein 1 that is encoded by the DHTKD1 gene. Thiamine Pyrophosphate 57-76 dehydrogenase E1 and transketolase domain containing 1 Homo sapiens 145-207 29191460-1 2018 Herein are reported unique properties of the novel human thiamin diphosphate (ThDP)-dependent enzyme 2-oxoadipate dehydrogenase (hE1a), known as dehydrogenase E1 and transketolase domain-containing protein 1 that is encoded by the DHTKD1 gene. Thiamine Pyrophosphate 57-76 dehydrogenase E1 and transketolase domain containing 1 Homo sapiens 231-237 29191460-1 2018 Herein are reported unique properties of the novel human thiamin diphosphate (ThDP)-dependent enzyme 2-oxoadipate dehydrogenase (hE1a), known as dehydrogenase E1 and transketolase domain-containing protein 1 that is encoded by the DHTKD1 gene. Thiamine Pyrophosphate 78-82 dehydrogenase E1 and transketolase domain containing 1 Homo sapiens 145-207 29191460-1 2018 Herein are reported unique properties of the novel human thiamin diphosphate (ThDP)-dependent enzyme 2-oxoadipate dehydrogenase (hE1a), known as dehydrogenase E1 and transketolase domain-containing protein 1 that is encoded by the DHTKD1 gene. Thiamine Pyrophosphate 78-82 dehydrogenase E1 and transketolase domain containing 1 Homo sapiens 231-237 28931541-2 2017 We have previously shown that human colonocytes possess an efficient carrier-mediated uptake process for TPP that involves the SLC44A4 system and this uptake process is adaptively regulated by prevailing extracellular TPP level. Thiamine Pyrophosphate 218-221 solute carrier family 44 member 4 Homo sapiens 127-134 28931541-8 2017 Similar changes in Slc44a4 mRNA expression were observed when mouse colonoids were maintained with TPP for short- and long-term periods. Thiamine Pyrophosphate 99-102 solute carrier family 44, member 4 Mus musculus 19-26 27773789-1 2016 Decreased thiamine and reduced activity of thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase (OGDH) cause neurodegeneration. Thiamine Pyrophosphate 43-63 oxoglutarate dehydrogenase Rattus norvegicus 111-115 28346710-10 2017 Quantitative RT-PCR analysis of TPP biosynthesis genes and genes encoding the TPP-dependent enzymes pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase and transketolase revealed that the transcript levels of these genes were upregulated in the pale1 mutant. Thiamine Pyrophosphate 32-35 Transketolase Arabidopsis thaliana 162-175 28346710-10 2017 Quantitative RT-PCR analysis of TPP biosynthesis genes and genes encoding the TPP-dependent enzymes pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase and transketolase revealed that the transcript levels of these genes were upregulated in the pale1 mutant. Thiamine Pyrophosphate 78-81 Transketolase Arabidopsis thaliana 162-175 28665968-4 2017 These findings may bear some features of thiamine-deficient mice generated by pyrithiamine injection and a thiamine-deficient diet, suggesting that the primary cause of THMD2 could be thiamine pyrophosphate (TPP) deficiency. Thiamine Pyrophosphate 184-206 solute carrier family 19 member 3 Homo sapiens 169-174 28665968-4 2017 These findings may bear some features of thiamine-deficient mice generated by pyrithiamine injection and a thiamine-deficient diet, suggesting that the primary cause of THMD2 could be thiamine pyrophosphate (TPP) deficiency. Thiamine Pyrophosphate 208-211 solute carrier family 19 member 3 Homo sapiens 169-174 28552632-2 2017 Previous studies demonstrated that TK catalysis is strictly dependent on thiamine pyrophosphate (TPP) and divalent ions such as Mg2+. Thiamine Pyrophosphate 73-95 uncharacterized protein Chlamydomonas reinhardtii 35-37 28552632-2 2017 Previous studies demonstrated that TK catalysis is strictly dependent on thiamine pyrophosphate (TPP) and divalent ions such as Mg2+. Thiamine Pyrophosphate 97-100 uncharacterized protein Chlamydomonas reinhardtii 35-37 27773789-1 2016 Decreased thiamine and reduced activity of thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase (OGDH) cause neurodegeneration. Thiamine Pyrophosphate 65-69 oxoglutarate dehydrogenase Rattus norvegicus 111-115 27773789-4 2016 According to the data, the astrocytic OGDH may be up-regulated by an increase in intracellular ThDP, while the neuroblastomal OGDH functions at full ThDP saturation. Thiamine Pyrophosphate 95-99 oxoglutarate dehydrogenase Rattus norvegicus 38-42 27773789-4 2016 According to the data, the astrocytic OGDH may be up-regulated by an increase in intracellular ThDP, while the neuroblastomal OGDH functions at full ThDP saturation. Thiamine Pyrophosphate 149-153 oxoglutarate dehydrogenase Rattus norvegicus 126-130 27773789-6 2016 Increased ThDP level in response to the OGDH inhibition presumably up-regulated the enzyme to compensate for a decrease in reducing power which occurred in SP-treated astrocytes. Thiamine Pyrophosphate 10-14 oxoglutarate dehydrogenase Rattus norvegicus 40-44 27488151-8 2016 RESULTS: TPP prevented hyperglycemia by increasing the amount of malondialdehyde and decreasing endogen antioxidants, including total glutathione, glutathione reductase, glutathione S-transferase and superoxide dismutase. Thiamine Pyrophosphate 9-12 hematopoietic prostaglandin D synthase Rattus norvegicus 170-195 27188525-2 2016 Mammalian cells obtain the vitamin from their surroundings, converted it to thiamin pyrophosphate (TPP) in the cytoplasm, followed by uptake of TPP by mitochondria via a carrier-mediated process that involves the MTPPT (product of the SLC25A19 gene). Thiamine Pyrophosphate 144-147 solute carrier family 25 member 19 Homo sapiens 235-243 26999808-1 2016 Thiamin is essential for normal metabolism in pancreatic acinar cells (PAC) and is obtained from their microenvironment through specific plasma-membrane transporters, converted to thiamin pyrophosphate (TPP) in the cytoplasm, followed by uptake of TPP by mitochondria through the mitochondrial TPP (MTPP) transporter (MTPPT; product of SLC25A19 gene). Thiamine Pyrophosphate 203-206 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 Mus musculus 336-344 26657515-7 2016 We also observed a severe deficiency of free-thiamine and low levels of thiamine diphosphate in fibroblasts from SLC19A3 patients. Thiamine Pyrophosphate 72-92 solute carrier family 19 member 3 Homo sapiens 113-120 26998737-1 2016 We propose the first computational model for transketolase (TK), a thiamine diphosphate (ThDP)-dependent enzyme, using a quantum mechanical/molecular mechanical method on the basis of crystallographic TK structures from yeast and Escherichia coli, together with experimental kinetic data reported in the literature with wild-type and mutant TK. Thiamine Pyrophosphate 67-87 transketolase Homo sapiens 45-58 26998737-1 2016 We propose the first computational model for transketolase (TK), a thiamine diphosphate (ThDP)-dependent enzyme, using a quantum mechanical/molecular mechanical method on the basis of crystallographic TK structures from yeast and Escherichia coli, together with experimental kinetic data reported in the literature with wild-type and mutant TK. Thiamine Pyrophosphate 89-93 transketolase Homo sapiens 45-58 26901654-2 2016 We have recently demonstrated the existence of an efficient and specific carrier-mediated uptake process for TPP in human colonocytes, identified the TPP transporter (TPPT) involved (product of the SLC44A4 gene), and shown that expression of TPPT along the gastrointestinal (GI) tract is restricted to the colon. Thiamine Pyrophosphate 109-112 solute carrier family 44 member 4 Homo sapiens 167-171 26901654-2 2016 We have recently demonstrated the existence of an efficient and specific carrier-mediated uptake process for TPP in human colonocytes, identified the TPP transporter (TPPT) involved (product of the SLC44A4 gene), and shown that expression of TPPT along the gastrointestinal (GI) tract is restricted to the colon. Thiamine Pyrophosphate 109-112 solute carrier family 44 member 4 Homo sapiens 242-246 26316591-2 2015 Cells obtain thiamin from their surroundings and enzymatically convert it into thiamin pyrophosphate (TPP) in the cytoplasm; TPP is then taken up by mitochondria via a specific carrier the mitochondrial TPP transporter (MTPPT; product of the SLC25A19 gene). Thiamine Pyrophosphate 79-100 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 Mus musculus 242-250 26455416-9 2015 TPP is also a cofactor for the enzyme 2-hydroxyacyl-CoA lyase (HACL1) in the peroxisome, emphasizing its importance in alpha oxidation and plasmalogen synthesis in cell membrane physiology. Thiamine Pyrophosphate 0-3 2-hydroxyacyl-CoA lyase 1 Homo sapiens 63-68 26316591-2 2015 Cells obtain thiamin from their surroundings and enzymatically convert it into thiamin pyrophosphate (TPP) in the cytoplasm; TPP is then taken up by mitochondria via a specific carrier the mitochondrial TPP transporter (MTPPT; product of the SLC25A19 gene). Thiamine Pyrophosphate 102-105 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 Mus musculus 242-250 26316591-2 2015 Cells obtain thiamin from their surroundings and enzymatically convert it into thiamin pyrophosphate (TPP) in the cytoplasm; TPP is then taken up by mitochondria via a specific carrier the mitochondrial TPP transporter (MTPPT; product of the SLC25A19 gene). Thiamine Pyrophosphate 125-128 solute carrier family 25 (mitochondrial thiamine pyrophosphate carrier), member 19 Mus musculus 242-250 26457526-0 2015 High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate. Thiamine Pyrophosphate 100-122 transketolase Lactobacillus salivarius UCC118 55-68 26457526-6 2015 The crystal structures of the megaplasmid-encoded transketolase with and without the enzyme cofactor thiamine pyrophosphate have been determined. Thiamine Pyrophosphate 101-123 transketolase Lactobacillus salivarius UCC118 50-63 25715703-2 2015 There is a specific high-affinity regulated carrier-mediated uptake system for TPP in human colonocytes (product of the SLC44A4 gene). Thiamine Pyrophosphate 79-82 solute carrier family 44 member 4 Homo sapiens 120-127 26212886-7 2015 Thiamin and ThDP regulate malate dehydrogenase isoforms and pyridoxal kinase. Thiamine Pyrophosphate 12-16 malic enzyme 1 Homo sapiens 26-46 24637331-2 2014 In Arabidopsis thaliana, THI1 protein has been associated with the synthesis of the thiazole ring, a finding supported by the identification of a thiamine pyrophosphate (TPP)-like compound in its structure. Thiamine Pyrophosphate 146-168 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) Arabidopsis thaliana 25-29 25316705-10 2015 CONCLUSION AND IMPLICATIONS: The cardiotoxic effect of doxorubicin originated from the inhibition of TPK enzyme resulting in insufficient production of thiamine pyrophosphate. Thiamine Pyrophosphate 152-174 thiamin pyrophosphokinase 1 Rattus norvegicus 101-104 25670766-4 2015 These phenotypes were complemented by germinating the seeds of transketolase-overexpressing lines in media containing either thiamine pyrophosphate or thiamine. Thiamine Pyrophosphate 125-147 Transketolase Arabidopsis thaliana 63-76 25670766-6 2015 When transketolase-overexpressing plants were supplemented with thiamine or thiamine pyrophosphate throughout the life cycle, they grew normally and the seed produced from these plants generated plants that did not have a growth or chlorotic phenotype. Thiamine Pyrophosphate 76-98 Transketolase Arabidopsis thaliana 5-18 24590690-5 2014 Though it was long thought that ThTP was synthesized by a ThDP:ATP phosphotransferase, more recent studies indicate that it can be synthesized by two different enzymes: (1) adenylate kinase 1 in the cytosol and (2) FoF1-ATP synthase in brain mitochondria. Thiamine Pyrophosphate 58-62 thiamine triphosphatase Homo sapiens 32-36 25267444-3 2014 In contrast, transketolase and phosphoketolase make use of the bioorganic cofactor thiamin diphosphate to cleave the preceding C2-C3 bond of ketose phosphates. Thiamine Pyrophosphate 83-102 transketolase Homo sapiens 13-26 24858398-3 2014 Hybrid nanocomplexes composed of chitosan, protamine, lecithin, and thiamine pyrophosphate were prepared for systemic delivery of survivin (SVN) siRNA. Thiamine Pyrophosphate 68-90 baculoviral IAP repeat-containing 5 Mus musculus 130-138 24637331-2 2014 In Arabidopsis thaliana, THI1 protein has been associated with the synthesis of the thiazole ring, a finding supported by the identification of a thiamine pyrophosphate (TPP)-like compound in its structure. Thiamine Pyrophosphate 170-173 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) Arabidopsis thaliana 25-29 24379411-4 2014 Here we report on the molecular identification of the colonic TPP uptake system as the product of the SLC44A4 gene. Thiamine Pyrophosphate 62-65 solute carrier family 44 member 4 Homo sapiens 102-109 24767541-1 2014 1-Deoxy-D-xylulose 5-phosphate (DXP) synthase catalyzes the formation of DXP from pyruvate and D-glyceraldehyde 3-phosphate (GraP) in a thiamin diphosphate-dependent manner, and is the first step in the essential pathway to isoprenoids in human pathogens. Thiamine Pyrophosphate 136-155 GRB2 related adaptor protein Homo sapiens 95-123 24767541-1 2014 1-Deoxy-D-xylulose 5-phosphate (DXP) synthase catalyzes the formation of DXP from pyruvate and D-glyceraldehyde 3-phosphate (GraP) in a thiamin diphosphate-dependent manner, and is the first step in the essential pathway to isoprenoids in human pathogens. Thiamine Pyrophosphate 136-155 GRB2 related adaptor protein Homo sapiens 125-129 24767541-8 2014 These results suggest a role of these residues in promoting GraP binding, which in turn facilitates decarboxylation, and also highlight interesting differences between DXP synthase and other thiamin diphosphate-dependent enzymes. Thiamine Pyrophosphate 191-210 GRB2 related adaptor protein Homo sapiens 60-64 24452394-6 2014 Recently, it has been shown that the thiamine coenzyme, thiamine pyrophosphate reduces PDK-mediated phosphorylation of PDH. Thiamine Pyrophosphate 56-78 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 119-122 24379411-5 2014 We cloned the cDNA of SLC44A4 from human colonic epithelial NCM460 cells, which, upon expression in ARPE19 cells, led to a significant (p < 0.01, >5-fold) induction in [(3)H]TPP uptake. Thiamine Pyrophosphate 180-183 solute carrier family 44 member 4 Homo sapiens 22-29 23688082-4 2014 Three cofactors are required for the activity of AHAS: thiamine diphosphate (ThDP), Mg2+ and flavin-adenine dinucleotide (FAD). Thiamine Pyrophosphate 55-75 ilvB acetolactate synthase like Homo sapiens 49-53 23632005-5 2014 TPP, especially at a dosage of 20 mg/kg, significantly reduced TBARS and MPO levels that increase with cisplatin administration compared with the thiamine group, while TPP significantly increases GSH and SOD levels. Thiamine Pyrophosphate 0-3 myeloperoxidase Rattus norvegicus 73-76 23688082-4 2014 Three cofactors are required for the activity of AHAS: thiamine diphosphate (ThDP), Mg2+ and flavin-adenine dinucleotide (FAD). Thiamine Pyrophosphate 77-81 ilvB acetolactate synthase like Homo sapiens 49-53 24114639-1 2013 INTRODUCTION: The activity of erythrocyte transketolase induced by thiamine pyrophosphate and normalized to age of the patient is a marker of thiamine metabolism disturbances with pathological consequences in the central and peripheral nervous system. Thiamine Pyrophosphate 67-89 transketolase Homo sapiens 42-55 23914308-1 2013 This review is focused on three types of enzymes decarboxylating very different substrates: (1) Thiamin diphosphate (ThDP)-dependent enzymes reacting with 2-oxo acids; (2) Pyridoxal phosphate (PLP)-dependent enzymes reacting with alpha-amino acids; and (3) An enzyme with no known co-factors, orotidine 5"-monophosphate decarboxylase (OMPDC). Thiamine Pyrophosphate 117-121 pyridoxal phosphatase Homo sapiens 193-196 23692511-9 2013 These results suggest that the Pdc1p phosphorylation dependent on SIT4 occurs at residues that change the apparent affinity for TPP and pyruvate. Thiamine Pyrophosphate 128-131 indolepyruvate decarboxylase 1 Saccharomyces cerevisiae S288C 31-36 23692511-9 2013 These results suggest that the Pdc1p phosphorylation dependent on SIT4 occurs at residues that change the apparent affinity for TPP and pyruvate. Thiamine Pyrophosphate 128-131 type 2A-related serine/threonine-protein phosphatase SIT4 Saccharomyces cerevisiae S288C 66-70 23440198-1 2013 A transketolase reaction was catalyzed by cyanide ion under prebiotic conditions instead of its modern catalyst, thiamine pyrophosphate (TPP). Thiamine Pyrophosphate 113-135 transketolase Homo sapiens 2-15 23440198-1 2013 A transketolase reaction was catalyzed by cyanide ion under prebiotic conditions instead of its modern catalyst, thiamine pyrophosphate (TPP). Thiamine Pyrophosphate 137-140 transketolase Homo sapiens 2-15 23261987-8 2013 In view of these results, it is unlikely that TKTL1 may be a ThDP-dependent protein capable of catalyzing the transketolase reaction, as hypothesized previously. Thiamine Pyrophosphate 61-65 transketolase like 1 Homo sapiens 46-51 24114639-14 2013 Abnormalities in transketolase activity, when expressed in three modalities: basal activity, normalized transketolase activity ratio and the activity after the stimulation with thiamine pyrophosphate may be differentiated as thiamine-dependent or resulting from posttranslational modification. Thiamine Pyrophosphate 177-199 transketolase Homo sapiens 17-30 23804074-3 2013 Overexpression of thiamine pyrophosphokinase (the enzyme that converts thiamine into its active form, thiamine pyrophosphate) hypersensitizes parasites to oxythiamine by up to 1,700-fold, consistent with oxythiamine being a substrate for thiamine pyrophosphokinase and its conversion into an antimetabolite. Thiamine Pyrophosphate 102-124 thiamine pyrophosphokinase Mus musculus 18-44 23804074-3 2013 Overexpression of thiamine pyrophosphokinase (the enzyme that converts thiamine into its active form, thiamine pyrophosphate) hypersensitizes parasites to oxythiamine by up to 1,700-fold, consistent with oxythiamine being a substrate for thiamine pyrophosphokinase and its conversion into an antimetabolite. Thiamine Pyrophosphate 102-124 thiamine pyrophosphokinase Mus musculus 238-264 23342634-2 2012 Experimental data on the regulation of transketolase activity with thiamin pyrophosphate (TPP) and its anticoenzyme analogues are presented. Thiamine Pyrophosphate 67-88 transketolase Rattus norvegicus 39-52 23341335-6 2013 Our model suggests that in Arabidopsis, the THIC promoter and the thiamin-pyrophosphate riboswitch act simultaneously to tightly regulate thiamin biosynthesis in a circadian manner and consequently sense and control vital points of core cellular metabolism. Thiamine Pyrophosphate 66-87 thiaminC Arabidopsis thaliana 44-48 22659053-1 2012 Thiamin pyrophosphokinase (TPK) converts thiamin to its active form, thiamin diphosphate. Thiamine Pyrophosphate 69-88 thiamin pyrophosphokinase 1 Oncorhynchus mykiss 0-25 22659053-1 2012 Thiamin pyrophosphokinase (TPK) converts thiamin to its active form, thiamin diphosphate. Thiamine Pyrophosphate 69-88 thiamin pyrophosphokinase 1 Oncorhynchus mykiss 27-30 23342634-2 2012 Experimental data on the regulation of transketolase activity with thiamin pyrophosphate (TPP) and its anticoenzyme analogues are presented. Thiamine Pyrophosphate 90-93 transketolase Rattus norvegicus 39-52 23342634-6 2012 The kinetics research findings illustrate the nonidentity of enzyme active sites with respect to TPP binding with transketolase. Thiamine Pyrophosphate 97-100 transketolase Rattus norvegicus 114-127 22404710-1 2012 In Saccharomyces cerevisiae, genes involved in thiamin pyrophosphate (TPP) synthesis (THI genes) and the pyruvate decarboxylase structural gene PDC5 are transcriptionally induced in response to thiamin starvation. Thiamine Pyrophosphate 70-73 indolepyruvate decarboxylase 5 Saccharomyces cerevisiae S288C 144-148 22426856-3 2012 Comparative genomic analysis indicated that two members of the mitochondrial carrier family (MCF) in Arabidopsis (At5g48970 and At3g21390) and two in maize (GRMZM2G118515 and GRMZM2G124911) are related to the ThDP carriers of animals and Saccharomyces cerevisiae. Thiamine Pyrophosphate 209-213 mitochondrial thiamine diphosphate carrier 1 Zea mays 157-170 22426856-4 2012 Expression of each of these plant proteins in a S. cerevisiae ThDP carrier (TPC1) null mutant complemented the growth defect on fermentable carbon sources and restored the level of mitochondrial ThDP and the activity of the mitochondrial ThDP-dependent enzyme acetolactate synthase. Thiamine Pyrophosphate 62-66 thiamine transporter TPC1 Saccharomyces cerevisiae S288C 76-80 22426856-4 2012 Expression of each of these plant proteins in a S. cerevisiae ThDP carrier (TPC1) null mutant complemented the growth defect on fermentable carbon sources and restored the level of mitochondrial ThDP and the activity of the mitochondrial ThDP-dependent enzyme acetolactate synthase. Thiamine Pyrophosphate 195-199 thiamine transporter TPC1 Saccharomyces cerevisiae S288C 76-80 22426856-4 2012 Expression of each of these plant proteins in a S. cerevisiae ThDP carrier (TPC1) null mutant complemented the growth defect on fermentable carbon sources and restored the level of mitochondrial ThDP and the activity of the mitochondrial ThDP-dependent enzyme acetolactate synthase. Thiamine Pyrophosphate 195-199 thiamine transporter TPC1 Saccharomyces cerevisiae S288C 76-80 22803947-3 2012 On the basis of the revealed differences in structures of these proteins, we assume it is unlikely that TKTL1 can be a thiamine diphosphate-dependent protein capable of catalyzing the transketolase reaction. Thiamine Pyrophosphate 119-139 transketolase like 1 Homo sapiens 104-109 22214485-5 2012 RESULTS: The expression of Arabidopsis genes involved in the thiamine diphosphate biosynthesis pathway, including that of THI1, THIC, TH1 and TPK, was analyzed for 48 h in seedlings subjected to NaCl or sorbitol treatment. Thiamine Pyrophosphate 61-81 thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) Arabidopsis thaliana 122-126 22214485-7 2012 The changes in these gene transcript levels were further found to correlate with increases in thiamine and its diphosphate ester content in seedlings, as well as with the enhancement of gene expression for enzymes which require thiamine diphosphate as a cofactor, mainly alpha-ketoglutarate dehydrogenase, pyruvate dehydrogenase and transketolase. Thiamine Pyrophosphate 228-248 Transketolase Arabidopsis thaliana 333-346 22214485-5 2012 RESULTS: The expression of Arabidopsis genes involved in the thiamine diphosphate biosynthesis pathway, including that of THI1, THIC, TH1 and TPK, was analyzed for 48 h in seedlings subjected to NaCl or sorbitol treatment. Thiamine Pyrophosphate 61-81 thiaminC Arabidopsis thaliana 128-132 22214485-5 2012 RESULTS: The expression of Arabidopsis genes involved in the thiamine diphosphate biosynthesis pathway, including that of THI1, THIC, TH1 and TPK, was analyzed for 48 h in seedlings subjected to NaCl or sorbitol treatment. Thiamine Pyrophosphate 61-81 thiamin biosynthesis protein Arabidopsis thaliana 134-137 21288652-3 2011 TPP is a relevant cofactor for transketolase (TK), alpha-ketoglutarate dehydrogenase (alphaKDH), and pyruvate dehydrogenase (PDH), all these enzymes are fundamental for glucose metabolism. Thiamine Pyrophosphate 0-3 transketolase Homo sapiens 31-44 21612515-4 2011 Thiamine is converted to thiamine diphosphate (TDP) by thiamine diphosphokinase (TDPK). Thiamine Pyrophosphate 25-45 thiamin pyrophosphokinase 1 Homo sapiens 55-79 21612515-4 2011 Thiamine is converted to thiamine diphosphate (TDP) by thiamine diphosphokinase (TDPK). Thiamine Pyrophosphate 25-45 thiamin pyrophosphokinase 1 Homo sapiens 81-85 21672592-0 2011 Acetyl-CoA metabolism in amprolium-evoked thiamine pyrophosphate deficits in cholinergic SN56 neuroblastoma cells. Thiamine Pyrophosphate 42-64 HPS3, biogenesis of lysosomal organelles complex 2 subunit 1 Mus musculus 7-10 21672592-2 2011 However, particular alterations in distribution of key acetylcholine precursor, acetyl-CoA, in the cholinergic neuron compartment of thiamine pyrophosphate-deficient brain remain unknown. Thiamine Pyrophosphate 133-155 HPS3, biogenesis of lysosomal organelles complex 2 subunit 1 Mus musculus 87-90 21672592-3 2011 Therefore, the aim of our work was to find out how amprolium-induced thiamine pyrophosphate deficits (TD) affect distribution of acetyl-CoA in the compartment of pure cholinergic neuroblastoma SN56 cells originating from murine septum. Thiamine Pyrophosphate 69-91 HPS3, biogenesis of lysosomal organelles complex 2 subunit 1 Mus musculus 136-139 21501384-1 2011 The product of the ARO10 gene from Saccharomyces cerevisiae was initially identified as a thiamine diphosphate-dependent phenylpyruvate decarboxylase with a broad substrate specificity. Thiamine Pyrophosphate 90-110 phenylpyruvate decarboxylase ARO10 Saccharomyces cerevisiae S288C 19-24 21288652-3 2011 TPP is a relevant cofactor for transketolase (TK), alpha-ketoglutarate dehydrogenase (alphaKDH), and pyruvate dehydrogenase (PDH), all these enzymes are fundamental for glucose metabolism. Thiamine Pyrophosphate 0-3 transketolase Homo sapiens 46-48 21288652-3 2011 TPP is a relevant cofactor for transketolase (TK), alpha-ketoglutarate dehydrogenase (alphaKDH), and pyruvate dehydrogenase (PDH), all these enzymes are fundamental for glucose metabolism. Thiamine Pyrophosphate 0-3 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 101-123 21288652-3 2011 TPP is a relevant cofactor for transketolase (TK), alpha-ketoglutarate dehydrogenase (alphaKDH), and pyruvate dehydrogenase (PDH), all these enzymes are fundamental for glucose metabolism. Thiamine Pyrophosphate 0-3 pyruvate dehydrogenase phosphatase catalytic subunit 1 Homo sapiens 125-128 20667822-1 2010 The crystal structure of human transketolase (TKT), a thiamine diphosphate (ThDP) and Ca(2+)-dependent enzyme that catalyzes the interketol transfer between ketoses and aldoses as part of the pentose phosphate pathway, has been determined to 1.75 A resolution. Thiamine Pyrophosphate 54-74 transketolase Homo sapiens 46-49 21370850-1 2011 Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. Thiamine Pyrophosphate 39-58 ilvB acetolactate synthase like Homo sapiens 0-26 21370850-1 2011 Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. Thiamine Pyrophosphate 39-58 ilvB acetolactate synthase like Homo sapiens 28-32 21370850-1 2011 Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. Thiamine Pyrophosphate 60-64 ilvB acetolactate synthase like Homo sapiens 0-26 21370850-1 2011 Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. Thiamine Pyrophosphate 60-64 ilvB acetolactate synthase like Homo sapiens 28-32 21552434-2 2011 TPP is the cofactor of metabolically important enzymes such as pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, branched-chain alpha-keto acid dehydrogenase, transketolase and 2-hydroxyphytanoyl-CoA lyase. Thiamine Pyrophosphate 0-3 oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide) Mus musculus 87-120 20667822-1 2010 The crystal structure of human transketolase (TKT), a thiamine diphosphate (ThDP) and Ca(2+)-dependent enzyme that catalyzes the interketol transfer between ketoses and aldoses as part of the pentose phosphate pathway, has been determined to 1.75 A resolution. Thiamine Pyrophosphate 54-74 transketolase Homo sapiens 31-44 20667822-1 2010 The crystal structure of human transketolase (TKT), a thiamine diphosphate (ThDP) and Ca(2+)-dependent enzyme that catalyzes the interketol transfer between ketoses and aldoses as part of the pentose phosphate pathway, has been determined to 1.75 A resolution. Thiamine Pyrophosphate 76-80 transketolase Homo sapiens 31-44 20667822-1 2010 The crystal structure of human transketolase (TKT), a thiamine diphosphate (ThDP) and Ca(2+)-dependent enzyme that catalyzes the interketol transfer between ketoses and aldoses as part of the pentose phosphate pathway, has been determined to 1.75 A resolution. Thiamine Pyrophosphate 76-80 transketolase Homo sapiens 46-49 20667822-5 2010 The cofactor and substrate binding sites of human TKT bear high resemblance to those of other TKTs but also feature unique properties, including two lysines and a serine that interact with the beta-phosphate of ThDP. Thiamine Pyrophosphate 211-215 transketolase Homo sapiens 50-53 20188835-5 2010 Benfotiamine administration increases the levels of intracellular thiamine diphosphate, a cofactor necessary for the activation transketolase, resulting in the reduction of tissue level of AGEs. Thiamine Pyrophosphate 66-86 transketolase Homo sapiens 128-141 20504042-1 2010 Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate-dependent enzyme that catalyzes the condensation of pyruvate with either another pyruvate molecule (product acetolactate) or 2-ketobutyrate (product acetohydroxybutyrate) as the first common step in the biosynthesis of branched-chain amino acids in plants, bacteria, algae, and fungi. Thiamine Pyrophosphate 39-58 ilvB acetolactate synthase like Homo sapiens 28-32 20583149-9 2010 SLC25A19 has been described as a mitochondria inner membrane transporter for both deoxynucleotides and thiamine pyrophosphate (TPP). Thiamine Pyrophosphate 103-125 solute carrier family 25 member 19 Homo sapiens 0-8 20583149-9 2010 SLC25A19 has been described as a mitochondria inner membrane transporter for both deoxynucleotides and thiamine pyrophosphate (TPP). Thiamine Pyrophosphate 127-130 solute carrier family 25 member 19 Homo sapiens 0-8 20583149-10 2010 The biochemical phenotype of MCPHA may be attributable to decreased activity of the three mitochondrial enzymes that require TPP as a cofactor: pyruvate dehydrogenase, alpha-ketoglutarate dehydrogenase, and branched chain amino acid dehydrogenase. Thiamine Pyrophosphate 125-128 solute carrier family 25 member 19 Homo sapiens 29-34 20673211-3 2010 The amount of active transketolase in the isolated protein preparation was correlated with the content of thiamine diphosphate (ThDP) determined in the same preparation. Thiamine Pyrophosphate 106-126 transketolase Homo sapiens 21-34 20673211-3 2010 The amount of active transketolase in the isolated protein preparation was correlated with the content of thiamine diphosphate (ThDP) determined in the same preparation. Thiamine Pyrophosphate 128-132 transketolase Homo sapiens 21-34 20673211-4 2010 Induced optical activity, facilitating studies of ThDP binding by the apoenzyme and measurement of the transketolase reaction at each stage, was detected by circular dichroism spectroscopy. Thiamine Pyrophosphate 50-54 transketolase Homo sapiens 103-116 20121944-7 2010 Reconstituted DmTpc1p transports thiamine pyrophosphate and, to a lesser extent, pyrophosphate, ADP, ATP and other nucleotides. Thiamine Pyrophosphate 33-55 Thiamine pyrophosphate carrier protein 1 Drosophila melanogaster 14-21 20121944-9 2010 The main role of DmTpc1p is to import thiamine pyrophosphate into mitochondria by exchange with intramitochondrial ATP and/or ADP. Thiamine Pyrophosphate 38-60 Thiamine pyrophosphate carrier protein 1 Drosophila melanogaster 17-24 19589233-6 2009 Coadministration of folate or its analogues, such as folinate and 5-methyltetrahydrofolate, substrates for both PCFT/HCP1 and RFC1, significantly suppressed the methotrexate influx at pH 5.5, whereas thiamine pyrophosphate, an inhibitor for RFC1 alone, exerted no significant effect. Thiamine Pyrophosphate 200-222 solute carrier family 46 member 1 Rattus norvegicus 112-121 19589233-6 2009 Coadministration of folate or its analogues, such as folinate and 5-methyltetrahydrofolate, substrates for both PCFT/HCP1 and RFC1, significantly suppressed the methotrexate influx at pH 5.5, whereas thiamine pyrophosphate, an inhibitor for RFC1 alone, exerted no significant effect. Thiamine Pyrophosphate 200-222 solute carrier family 19 member 1 Rattus norvegicus 126-130 19420697-0 2009 Inhibition of branched-chain alpha-ketoacid dehydrogenase kinase by thiamine pyrophosphate at different potassium ionic levels. Thiamine Pyrophosphate 68-90 branched chain keto acid dehydrogenase kinase Homo sapiens 14-64 19420697-1 2009 Inhibition of branched-chain alpha-ketoacid dehydrogenase kinase (BDK) by thiamine pyrophosphate (TPP) was analyzed at two potassium ion (K(+)) concentrations. Thiamine Pyrophosphate 74-96 branched chain keto acid dehydrogenase kinase Homo sapiens 14-64 19420697-1 2009 Inhibition of branched-chain alpha-ketoacid dehydrogenase kinase (BDK) by thiamine pyrophosphate (TPP) was analyzed at two potassium ion (K(+)) concentrations. Thiamine Pyrophosphate 74-96 branched chain keto acid dehydrogenase kinase Homo sapiens 66-69 19420697-1 2009 Inhibition of branched-chain alpha-ketoacid dehydrogenase kinase (BDK) by thiamine pyrophosphate (TPP) was analyzed at two potassium ion (K(+)) concentrations. Thiamine Pyrophosphate 98-101 branched chain keto acid dehydrogenase kinase Homo sapiens 14-64 19420697-1 2009 Inhibition of branched-chain alpha-ketoacid dehydrogenase kinase (BDK) by thiamine pyrophosphate (TPP) was analyzed at two potassium ion (K(+)) concentrations. Thiamine Pyrophosphate 98-101 branched chain keto acid dehydrogenase kinase Homo sapiens 66-69 19420697-3 2009 These results suggest that BDK is less sensitive to TPP inhibition under physiological TPP and K(+) concentrations. Thiamine Pyrophosphate 52-55 branched chain keto acid dehydrogenase kinase Homo sapiens 27-30 19420697-3 2009 These results suggest that BDK is less sensitive to TPP inhibition under physiological TPP and K(+) concentrations. Thiamine Pyrophosphate 87-90 branched chain keto acid dehydrogenase kinase Homo sapiens 27-30 18490188-2 2009 In its active form, thiamin pyrophosphate (TPP), it is a co-enzyme for several enzymes, including transketolase. Thiamine Pyrophosphate 20-41 transketolase Homo sapiens 98-111 18490188-2 2009 In its active form, thiamin pyrophosphate (TPP), it is a co-enzyme for several enzymes, including transketolase. Thiamine Pyrophosphate 43-46 transketolase Homo sapiens 98-111 19290028-1 2009 Transketolase (TK), a thiamine diphosphate (ThDP)-dependent enzyme, catalyzes several key reactions of non-oxidative branch of pentose phosphate pathway. Thiamine Pyrophosphate 22-42 transketolase Homo sapiens 0-13 19290028-1 2009 Transketolase (TK), a thiamine diphosphate (ThDP)-dependent enzyme, catalyzes several key reactions of non-oxidative branch of pentose phosphate pathway. Thiamine Pyrophosphate 22-42 transketolase Homo sapiens 15-17 19290028-1 2009 Transketolase (TK), a thiamine diphosphate (ThDP)-dependent enzyme, catalyzes several key reactions of non-oxidative branch of pentose phosphate pathway. Thiamine Pyrophosphate 44-48 transketolase Homo sapiens 0-13 19290028-1 2009 Transketolase (TK), a thiamine diphosphate (ThDP)-dependent enzyme, catalyzes several key reactions of non-oxidative branch of pentose phosphate pathway. Thiamine Pyrophosphate 44-48 transketolase Homo sapiens 15-17 19290028-3 2009 Both ThDP and bivalent cations are strictly needed for TK activation, just like that for all ThDP-dependent enzymes. Thiamine Pyrophosphate 5-9 transketolase Homo sapiens 55-57 19290028-3 2009 Both ThDP and bivalent cations are strictly needed for TK activation, just like that for all ThDP-dependent enzymes. Thiamine Pyrophosphate 93-97 transketolase Homo sapiens 55-57 19364324-1 2009 In this work, we investigated the rate of formation of the central intermediate of the transketolase reaction with thiamine diphosphate (ThDP) or 4"-methylamino-ThDP as cofactors and its stability using stopped-flow spectroscopy and circular dichroism (CD) spectroscopy. Thiamine Pyrophosphate 115-135 transketolase Homo sapiens 87-100 19364324-1 2009 In this work, we investigated the rate of formation of the central intermediate of the transketolase reaction with thiamine diphosphate (ThDP) or 4"-methylamino-ThDP as cofactors and its stability using stopped-flow spectroscopy and circular dichroism (CD) spectroscopy. Thiamine Pyrophosphate 137-141 transketolase Homo sapiens 87-100 19364324-6 2009 These data suggest that transketolase in the complex with the NH2-methylated ThDP exhibits dihydroxyethyl-4"-methylamino-ThDP-synthase activity. Thiamine Pyrophosphate 77-81 transketolase Homo sapiens 24-37 19111488-5 2009 In addition, an interaction analysis of its cofactor (thiamine pyrophosphate) and a binding site description were carried out, suggesting the substrate channel already identified in yeast Transketolase. Thiamine Pyrophosphate 54-76 transketolase Homo sapiens 188-201 19013460-3 2008 Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. Thiamine Pyrophosphate 11-14 branched-chain-2-oxoacid decarboxylase THI3 Saccharomyces cerevisiae S288C 0-5 19013460-3 2008 Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. Thiamine Pyrophosphate 73-76 branched-chain-2-oxoacid decarboxylase THI3 Saccharomyces cerevisiae S288C 0-5 19187232-3 2009 AHAS requires the cofactors thiamine diphosphate (ThDP), Mg(2+) and FAD for activity. Thiamine Pyrophosphate 28-48 chlorsulfuron/imidazolinone resistant 1 Arabidopsis thaliana 0-4 19187232-3 2009 AHAS requires the cofactors thiamine diphosphate (ThDP), Mg(2+) and FAD for activity. Thiamine Pyrophosphate 50-54 chlorsulfuron/imidazolinone resistant 1 Arabidopsis thaliana 0-4 19187232-10 2009 Unlike the structures of Arabidopsis thaliana AHAS in complex with the classic disubstituted sulfonylureas, where ThDP is broken, this cofactor is intact and present most likely as the hydroxylethyl intermediate. Thiamine Pyrophosphate 114-118 chlorsulfuron/imidazolinone resistant 1 Arabidopsis thaliana 46-50 19140682-2 2009 Addition of 3-PKB resulted in the appearance of two transient intermediates formed consecutively, the first one to be formed a predecarboxylation ThDP-bound intermediate with lambda(max) at 477 nm, and the second one corresponding to the first postdecarboxylation intermediate the enamine with lambda(max) at 437 nm. Thiamine Pyrophosphate 146-150 AKT serine/threonine kinase 1 Homo sapiens 14-17 19140682-3 2009 The time course of formation/depletion of the PKB-ThDP covalent complex and of the enamine showed that decarboxylation was slower than formation of the PKB-ThDP covalent adduct. Thiamine Pyrophosphate 50-54 AKT serine/threonine kinase 1 Homo sapiens 46-49 19140682-3 2009 The time course of formation/depletion of the PKB-ThDP covalent complex and of the enamine showed that decarboxylation was slower than formation of the PKB-ThDP covalent adduct. Thiamine Pyrophosphate 156-160 AKT serine/threonine kinase 1 Homo sapiens 152-155