PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 2576285-5 1989 p-Anisidine and p-nitroaniline induced swelling of the tubular epithelial cells and a significant elevation in urinary NAG activities in rats, which was also caused by p-chloronitrobenzene. 4-nitroaniline 16-30 O-GlcNAcase Rattus norvegicus 119-122 2531734-3 1989 The scu-PA solution was activated by plasmin to two-chain u-PA, which releases p-nitroaniline from pyro-Glu-Gly-Arg-pNA, and the optical density at 405 nm was measured. 4-nitroaniline 79-93 plasminogen Homo sapiens 37-44 2525884-2 1989 para-Nitroaniline released by the cleavage of the lysine - p-nitroaniline bound by plasmin was derivatized to its diazonium salt and subsequently coupled to N-1-napthylethylenediamine in situ to form a diazoamino of an intense red color at the site of the plasminogen activator. 4-nitroaniline 0-17 plasminogen Homo sapiens 83-90 2531734-3 1989 The scu-PA solution was activated by plasmin to two-chain u-PA, which releases p-nitroaniline from pyro-Glu-Gly-Arg-pNA, and the optical density at 405 nm was measured. 4-nitroaniline 79-93 plasminogen activator, urokinase Homo sapiens 6-10 2461739-6 1988 Serine proteinases with suitable specificity are able to remove 4-nitroaniline or 7-amino-4-methylcoumarin from these aprotinin derivatives while at the same time resynthesis of the reactive-site peptide bond occurs. 4-nitroaniline 64-78 pancreatic trypsin inhibitor Bos taurus 118-127 3603422-1 1987 A chromogenic substrate, H-D-Phe-Pip-Arg-pNA (S-2238) is a highly specific substrate to thrombin and releases p-nitroaniline (pNA) by the action of thrombin. 4-nitroaniline 110-124 prolactin induced protein Homo sapiens 33-36 3415222-2 1988 The method is based on the aminopeptidase activity of these bacteria, which cleaves L-alanine-p-nitroanilide to yield p-nitroaniline, which is easily determined spectrophotometrically. 4-nitroaniline 118-132 carboxypeptidase Q Homo sapiens 27-41 3038961-6 1987 C1q reduced the activation of HF by ellagic acid, as measured by the release of p-nitroaniline from the synthetic substrate H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide dihydrochloride, an effect inhibited by monoclonal anti-human C1q murine IgG and by digestion of C1q by collagenase. 4-nitroaniline 80-94 complement C1q A chain Homo sapiens 0-3 3038961-6 1987 C1q reduced the activation of HF by ellagic acid, as measured by the release of p-nitroaniline from the synthetic substrate H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide dihydrochloride, an effect inhibited by monoclonal anti-human C1q murine IgG and by digestion of C1q by collagenase. 4-nitroaniline 80-94 coagulation factor XII Homo sapiens 30-32 3038961-6 1987 C1q reduced the activation of HF by ellagic acid, as measured by the release of p-nitroaniline from the synthetic substrate H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide dihydrochloride, an effect inhibited by monoclonal anti-human C1q murine IgG and by digestion of C1q by collagenase. 4-nitroaniline 80-94 complement C1q A chain Homo sapiens 238-241 3038961-6 1987 C1q reduced the activation of HF by ellagic acid, as measured by the release of p-nitroaniline from the synthetic substrate H-D-prolyl-L-phenylalanyl-L-arginine-p-nitroanilide dihydrochloride, an effect inhibited by monoclonal anti-human C1q murine IgG and by digestion of C1q by collagenase. 4-nitroaniline 80-94 complement C1q A chain Homo sapiens 238-241 3603422-1 1987 A chromogenic substrate, H-D-Phe-Pip-Arg-pNA (S-2238) is a highly specific substrate to thrombin and releases p-nitroaniline (pNA) by the action of thrombin. 4-nitroaniline 110-124 coagulation factor II, thrombin Homo sapiens 88-96 3603422-1 1987 A chromogenic substrate, H-D-Phe-Pip-Arg-pNA (S-2238) is a highly specific substrate to thrombin and releases p-nitroaniline (pNA) by the action of thrombin. 4-nitroaniline 110-124 coagulation factor II, thrombin Homo sapiens 148-156 6838889-4 1983 Poly(ethylene glycol) and bovine albumin, two additives frequently employed to eliminate enzyme loss from adsorption to cuvette and dilution vessel surfaces, also induce shifts in the p-nitroaniline spectrum. 4-nitroaniline 184-198 albumin Bos taurus 33-40 2876048-1 1986 Dipeptidyl-peptidase IV (EC 3.4.14.5) can be assayed relatively specifically in crude biological material by determination of the initial rate of formation of 4-nitroaniline from aminoacyl-proline-4-nitroanilides. 4-nitroaniline 159-173 dipeptidyl peptidase 4 Homo sapiens 0-23 6348027-4 1983 The protease shows a preference for leucine in the carboxylic side of the scissile bond of the substrate, cleaving the B-chain of oxidized bovine insulin only at the Leu15-Tyr16 bond and liberating p-nitroaniline from L-pyroglutamyl-L-alanyl-L-alanyl-L-leucine-p-nitroanilide. 4-nitroaniline 198-212 insulin Bos taurus 146-153 6210467-3 1982 To measure plasmin generation in these samples, we used the chromogenic plasmin substrate D-Val-Leu-Lys-p-nitroanilide, which liberates p-nitroaniline upon cleavage. 4-nitroaniline 136-150 plasminogen Homo sapiens 11-18 6210467-3 1982 To measure plasmin generation in these samples, we used the chromogenic plasmin substrate D-Val-Leu-Lys-p-nitroanilide, which liberates p-nitroaniline upon cleavage. 4-nitroaniline 136-150 plasminogen Homo sapiens 72-79 7377131-3 1980 The thrombin remaining after neutralization by AT-III cleaves a chromophore, p-nitroaniline, from the substrate, which can then te quantified in a spectrophotometer. 4-nitroaniline 77-91 coagulation factor II, thrombin Homo sapiens 4-12 7377131-3 1980 The thrombin remaining after neutralization by AT-III cleaves a chromophore, p-nitroaniline, from the substrate, which can then te quantified in a spectrophotometer. 4-nitroaniline 77-91 serpin family C member 1 Homo sapiens 47-53 497395-4 1979 The assays of factor Xa and thrombin, which measure the initial rate of p-nitroaniline release from S-2222 and S-2238, respectively, showed similar small amounts of factor Xa (4-34 ng/ml) and thrombin (12-76 ng/ml) in the activated and unactivated concentrates. 4-nitroaniline 72-86 coagulation factor X Homo sapiens 14-23 497395-4 1979 The assays of factor Xa and thrombin, which measure the initial rate of p-nitroaniline release from S-2222 and S-2238, respectively, showed similar small amounts of factor Xa (4-34 ng/ml) and thrombin (12-76 ng/ml) in the activated and unactivated concentrates. 4-nitroaniline 72-86 coagulation factor II, thrombin Homo sapiens 28-36 436285-2 1979 The thrombin generated is allowed to split the chromogenic substrate Tol-Gly-Pro-Arg-pNA yielding p-nitroaniline. 4-nitroaniline 98-112 coagulation factor II, thrombin Homo sapiens 4-12 598749-3 1977 By the action of factor Xa, p-nitroaniline (pNA) is split form the peptide bond. 4-nitroaniline 28-42 coagulation factor X Homo sapiens 17-26 598749-3 1977 By the action of factor Xa, p-nitroaniline (pNA) is split form the peptide bond. 4-nitroaniline 44-47 coagulation factor X Homo sapiens 17-26 1168550-6 1975 Reaction of oxytocinase on the substrate liberated p-nitroaniline, the absorption of which is measured at 410nm. 4-nitroaniline 51-65 leucyl and cystinyl aminopeptidase Homo sapiens 12-23 32814078-1 2020 Snake venom prothrombin activators such as Ecarin are readily assayed by continuous spectrophotometric monitoring of p-nitroaniline production in a one step assay containing prothrombin and a p-nitroanilide peptide substrate for thrombin. 4-nitroaniline 117-131 coagulation factor II, thrombin Homo sapiens 15-23 30981774-0 2019 Voltammetric determination of nitro compound 4-nitroaniline in aqueous medium at chitosan gelified modified carbon paste electrode (CS@CPE). 4-nitroaniline 45-59 carboxypeptidase E Homo sapiens 135-138 30981774-1 2019 A sensitive, selective and reproducible electrochemical method has been established for the electroanalysis of 4-nitroaniline (4-NA) using a carbon paste electrode modified with a chitosan solution gelled in acetic acid (CS@CPE). 4-nitroaniline 111-125 carboxypeptidase E Homo sapiens 224-227 30981774-1 2019 A sensitive, selective and reproducible electrochemical method has been established for the electroanalysis of 4-nitroaniline (4-NA) using a carbon paste electrode modified with a chitosan solution gelled in acetic acid (CS@CPE). 4-nitroaniline 127-131 carboxypeptidase E Homo sapiens 224-227 30981774-7 2019 The repeatability of the current peak registered at CS@CPE was performed at a level of 0.5muM 4-NA employing one sensor on the same day for eight measurements. 4-nitroaniline 94-98 carboxypeptidase E Homo sapiens 55-58 30696905-4 2019 The GGT-catalyzed hydrolysis of GPNA produced cytotoxic effects in lung cancer A549 cells, resulting from the release of metabolite p-nitroaniline (PNA) rather than from the inhibition of Gln uptake. 4-nitroaniline 132-146 gamma-glutamyltransferase 2, pseudogene Homo sapiens 4-7 30696905-4 2019 The GGT-catalyzed hydrolysis of GPNA produced cytotoxic effects in lung cancer A549 cells, resulting from the release of metabolite p-nitroaniline (PNA) rather than from the inhibition of Gln uptake. 4-nitroaniline 33-36 gamma-glutamyltransferase 2, pseudogene Homo sapiens 4-7 30107333-0 2018 Enhanced photocatalytic activity of CdS/SnS2 nanocomposite with highly-efficient charge transfer and visible light utilization for selective reduction of 4-nitroaniline. 4-nitroaniline 154-168 CDP-diacylglycerol synthase 1 Homo sapiens 36-39 30107333-0 2018 Enhanced photocatalytic activity of CdS/SnS2 nanocomposite with highly-efficient charge transfer and visible light utilization for selective reduction of 4-nitroaniline. 4-nitroaniline 154-168 sodium voltage-gated channel alpha subunit 11 Homo sapiens 40-44 30107333-6 2018 Resultantly, the prepared CdS/SnS2 composites exhibit high conversion efficiency and selectivity on 4-nitroaniline (4-NA) reduction in the aqueous solution containing ammonium formate under visible light irradiation, which can reduce almost all 4-NA within 12 min. 4-nitroaniline 100-114 CDP-diacylglycerol synthase 1 Homo sapiens 26-29 30107333-6 2018 Resultantly, the prepared CdS/SnS2 composites exhibit high conversion efficiency and selectivity on 4-nitroaniline (4-NA) reduction in the aqueous solution containing ammonium formate under visible light irradiation, which can reduce almost all 4-NA within 12 min. 4-nitroaniline 100-114 sodium voltage-gated channel alpha subunit 11 Homo sapiens 30-34 30107333-6 2018 Resultantly, the prepared CdS/SnS2 composites exhibit high conversion efficiency and selectivity on 4-nitroaniline (4-NA) reduction in the aqueous solution containing ammonium formate under visible light irradiation, which can reduce almost all 4-NA within 12 min. 4-nitroaniline 116-120 CDP-diacylglycerol synthase 1 Homo sapiens 26-29 30107333-6 2018 Resultantly, the prepared CdS/SnS2 composites exhibit high conversion efficiency and selectivity on 4-nitroaniline (4-NA) reduction in the aqueous solution containing ammonium formate under visible light irradiation, which can reduce almost all 4-NA within 12 min. 4-nitroaniline 116-120 sodium voltage-gated channel alpha subunit 11 Homo sapiens 30-34 30107333-6 2018 Resultantly, the prepared CdS/SnS2 composites exhibit high conversion efficiency and selectivity on 4-nitroaniline (4-NA) reduction in the aqueous solution containing ammonium formate under visible light irradiation, which can reduce almost all 4-NA within 12 min. 4-nitroaniline 245-249 CDP-diacylglycerol synthase 1 Homo sapiens 26-29 30107333-6 2018 Resultantly, the prepared CdS/SnS2 composites exhibit high conversion efficiency and selectivity on 4-nitroaniline (4-NA) reduction in the aqueous solution containing ammonium formate under visible light irradiation, which can reduce almost all 4-NA within 12 min. 4-nitroaniline 245-249 sodium voltage-gated channel alpha subunit 11 Homo sapiens 30-34 30107333-8 2018 The 4-NA are effectively photo-reduced by the synergistic effect of electrons and CO2- radicals. 4-nitroaniline 4-8 complement C2 Homo sapiens 82-85 28502180-4 2017 We show the performance of the films for SERS detection of bacterial quorum sensing molecules in culture medium, and catalytic properties are demonstrated through the reduction of 4-nitroaniline. 4-nitroaniline 180-194 seryl-tRNA synthetase 2, mitochondrial Homo sapiens 41-45 29408178-4 2018 D-MAP is cleaved by FAAH with an 8-fold larger specificity constant than the previously reported octanoyl-analog Oc-MAP (Vmax/Km of 1.09 and 0.134 mL min-1 mg-1, respectively), with both MAP derivatives possessing superior substrate properties and much increased aqueous solubility compared to the respective p-nitroaniline compounds D-pNA and Oc-pNA. 4-nitroaniline 309-323 fatty acid amide hydrolase Homo sapiens 20-24 29411223-3 2018 In this assay, the activation of factor X leads to the formation of activated thrombin from prothrombin and the eventual hydrolyses of a thrombin chromogenic substrate which contains a p-nitroaniline leaving group. 4-nitroaniline 185-199 coagulation factor II, thrombin Homo sapiens 78-86 29411223-3 2018 In this assay, the activation of factor X leads to the formation of activated thrombin from prothrombin and the eventual hydrolyses of a thrombin chromogenic substrate which contains a p-nitroaniline leaving group. 4-nitroaniline 185-199 coagulation factor II, thrombin Homo sapiens 95-103 28405804-5 2017 In this present study, we found that the specific tPA inhibitor, plasminogen activator inhibitor-1 (PAI-1; 0.25 muM), significantly (p < 0.0001) inhibited 4-nitroaniline release, by 97.74% during the 10-min duration of the assay, which is indicative of tPA protease inhibition. 4-nitroaniline 158-172 serpin family E member 1 Homo sapiens 65-98 28405804-5 2017 In this present study, we found that the specific tPA inhibitor, plasminogen activator inhibitor-1 (PAI-1; 0.25 muM), significantly (p < 0.0001) inhibited 4-nitroaniline release, by 97.74% during the 10-min duration of the assay, which is indicative of tPA protease inhibition. 4-nitroaniline 158-172 serpin family E member 1 Homo sapiens 100-105 26915974-3 2016 We found that HuBChE contributed up to 90% of the yellow p-nitroaniline product in a standard endotoxin assay through the catalytic hydrolysis of Ac-IEAR-pNA with a rate constant of 0.016 min(-1) and a Km of 2.9 mM in potassium phosphate buffer pH 7.0 at 24 C. Thus, endotoxin concentrations for native BChE are artificially high in the kinetic chromogenic assay. 4-nitroaniline 57-71 butyrylcholinesterase Homo sapiens 16-20 26585348-1 2016 Water soluble perylenediimide based fluorophore salt, N,N"-bis(ethelenetrimethyl ammoniumiodide)-perylene-3,4,9,10-tetracarboxylicbisimide (PDI-1), has been used for selective fluorescence sensing of picric acid (PA) and 4-nitroaniline (4-NA) in organic as well as aqueous medium across wide pH range (1.0 to 10.0). 4-nitroaniline 237-241 peptidyl arginine deiminase 1 Homo sapiens 140-145 26585348-3 2016 Addition of picric acid (PA) and 4-nitroaniline (4-NA) into PDI-1 in DMF/aqueous solution selectively quenches the fluorescence. 4-nitroaniline 33-47 peptidyl arginine deiminase 1 Homo sapiens 60-65 26585348-1 2016 Water soluble perylenediimide based fluorophore salt, N,N"-bis(ethelenetrimethyl ammoniumiodide)-perylene-3,4,9,10-tetracarboxylicbisimide (PDI-1), has been used for selective fluorescence sensing of picric acid (PA) and 4-nitroaniline (4-NA) in organic as well as aqueous medium across wide pH range (1.0 to 10.0). 4-nitroaniline 221-235 peptidyl arginine deiminase 1 Homo sapiens 140-145 26585348-3 2016 Addition of picric acid (PA) and 4-nitroaniline (4-NA) into PDI-1 in DMF/aqueous solution selectively quenches the fluorescence. 4-nitroaniline 49-53 peptidyl arginine deiminase 1 Homo sapiens 60-65 26585348-6 2016 Interestingly, PDI-1 showed selective fluorescence sensing of PA and 4-NA across wide pH range (1.0 to 10.0). 4-nitroaniline 69-73 peptidyl arginine deiminase 1 Homo sapiens 15-20 26585348-7 2016 Selective fluorescence sensing of PA and 4-NA has also been observed with trifluoroacetate (PDI-2), sulfate (PDI-3) salt of PDI-1 as well as octyl chain substituted PDI (PDI-4) without amine functionality. 4-nitroaniline 41-45 peptidyl arginine deiminase 2 Homo sapiens 92-97 26585348-7 2016 Selective fluorescence sensing of PA and 4-NA has also been observed with trifluoroacetate (PDI-2), sulfate (PDI-3) salt of PDI-1 as well as octyl chain substituted PDI (PDI-4) without amine functionality. 4-nitroaniline 41-45 peptidyl arginine deiminase 3 Homo sapiens 109-114 26585348-7 2016 Selective fluorescence sensing of PA and 4-NA has also been observed with trifluoroacetate (PDI-2), sulfate (PDI-3) salt of PDI-1 as well as octyl chain substituted PDI (PDI-4) without amine functionality. 4-nitroaniline 41-45 peptidyl arginine deiminase 1 Homo sapiens 124-129 26585348-7 2016 Selective fluorescence sensing of PA and 4-NA has also been observed with trifluoroacetate (PDI-2), sulfate (PDI-3) salt of PDI-1 as well as octyl chain substituted PDI (PDI-4) without amine functionality. 4-nitroaniline 41-45 peptidyl arginine deiminase 4 Homo sapiens 92-95 26585348-7 2016 Selective fluorescence sensing of PA and 4-NA has also been observed with trifluoroacetate (PDI-2), sulfate (PDI-3) salt of PDI-1 as well as octyl chain substituted PDI (PDI-4) without amine functionality. 4-nitroaniline 41-45 peptidyl arginine deiminase 4 Homo sapiens 170-175 26585348-8 2016 These studies suggest that PA and 4-NA might be having preferential interaction with PDI aromatic core and quenches the fluorescence. 4-nitroaniline 34-38 peptidyl arginine deiminase 4 Homo sapiens 85-88 26579994-6 2016 Of the 14 PPD-positive test patients, 5 (36%) reacted to 4-nitroaniline and 9 (64%) reacted to 4,4"-azodianiline. 4-nitroaniline 57-71 4-hydroxyphenylpyruvate dioxygenase Homo sapiens 10-13 26579994-7 2016 CONCLUSION: The results show that 4-nitroaniline and 4,4"-azodianiline, formed during oxidation of PPD, are potent sensitizers. 4-nitroaniline 34-48 4-hydroxyphenylpyruvate dioxygenase Homo sapiens 99-102 26579994-8 2016 PPD-sensitized patients react to a high extent to concentrations equimolar to PPD of 4-nitroaniline and 4,4"-azodianiline. 4-nitroaniline 85-99 4-hydroxyphenylpyruvate dioxygenase Homo sapiens 0-3 26579994-8 2016 PPD-sensitized patients react to a high extent to concentrations equimolar to PPD of 4-nitroaniline and 4,4"-azodianiline. 4-nitroaniline 85-99 4-hydroxyphenylpyruvate dioxygenase Homo sapiens 78-81 27509965-6 2016 Caspase3, 8 and 9 activities were enhanced as evidenced by colorimetric detection of paranitroaniline tagged with a substrate for each caspase. 4-nitroaniline 85-101 caspase 3 Homo sapiens 0-8 16305214-2 2005 Simulations are carried out for a model charge-transfer optical dye (p-nitroaniline) in SPC/E water in a range of temperatures down to the point of solvent ideal glass transition. 4-nitroaniline 69-83 proline rich protein gene cluster Homo sapiens 88-91 26134908-4 2015 The synthetic substrates contain a caspase cleavage site conjugated to a fluorochrome, such as 7-amino-4-methylcoumarin (AMC), or a chromophore, such as p-nitroaniline (pNA), for colorimetric detection. 4-nitroaniline 169-172 Death caspase-1 Drosophila melanogaster 35-42 25296712-3 2015 Caspase-3 activity assay was performed by measuring the pNA (p-nitroaniline) to indirectly reveal the catalytic activity of caspase-3. 4-nitroaniline 56-59 caspase 3 Homo sapiens 0-9 25296712-3 2015 Caspase-3 activity assay was performed by measuring the pNA (p-nitroaniline) to indirectly reveal the catalytic activity of caspase-3. 4-nitroaniline 61-75 caspase 3 Homo sapiens 0-9 25261660-5 2014 The activity of caspase3 was examined by the absorbance of p-nitroaniline (pNA) production. 4-nitroaniline 59-73 caspase 3 Homo sapiens 16-24 25261660-5 2014 The activity of caspase3 was examined by the absorbance of p-nitroaniline (pNA) production. 4-nitroaniline 75-78 caspase 3 Homo sapiens 16-24 25095637-9 2014 In case of cathepsin B activity, measured as the level of released p-nitroaniline, decreased, 2 weeks and 6 weeks after the surgical treatment in both cases of disease severity. 4-nitroaniline 67-81 cathepsin B Homo sapiens 11-22 24798487-4 2014 In this method, p-nitroaniline (pNA) released from Asp-Glu-Val-Asp-pNA by caspase-3 enzyme reaction was measured with DPV by using a glassy carbon electrode. 4-nitroaniline 16-30 caspase 3 Homo sapiens 74-83 24798487-4 2014 In this method, p-nitroaniline (pNA) released from Asp-Glu-Val-Asp-pNA by caspase-3 enzyme reaction was measured with DPV by using a glassy carbon electrode. 4-nitroaniline 32-35 caspase 3 Homo sapiens 74-83 25796931-4 2014 It was shown that the 4-nitroanilin thus obtained can be purified from the co-extracted substances on a L 40/100 mcm silicagel column. 4-nitroaniline 22-35 ubiquitin A-52 residue ribosomal protein fusion product 1 Homo sapiens 104-112 22388283-5 2012 DPP IV activity was determined by measuring the cleavage of chromogenic free 4-nitroaniline from Gly-Pro-p-nitroanilide at 405 nm with an ELISA plate reader. 4-nitroaniline 77-91 dipeptidyl peptidase 4 Homo sapiens 0-6 21793589-0 2011 Solvent-induced shift of the lowest singlet pi pi* charge-transfer excited state of p-nitroaniline in water: an application of the TDDFT/EFP1 method. 4-nitroaniline 86-100 thioredoxin domain containing 11 Homo sapiens 139-143 21793589-1 2011 The combined time-dependent density functional theory effective fragment potential method (TDDFT/EFP1) is applied to a study of the solvent-induced shift of the lowest singlet pi pi* charge-transfer excited state of p-nitroaniline (pNA) from the gas to the condensed phase in water. 4-nitroaniline 218-232 thioredoxin domain containing 11 Homo sapiens 97-101 21793589-1 2011 The combined time-dependent density functional theory effective fragment potential method (TDDFT/EFP1) is applied to a study of the solvent-induced shift of the lowest singlet pi pi* charge-transfer excited state of p-nitroaniline (pNA) from the gas to the condensed phase in water. 4-nitroaniline 234-237 thioredoxin domain containing 11 Homo sapiens 97-101 17623271-1 2007 The interactions of 2-nitroaniline (2-NA), 3-nitroaniline (3-NA) and 4-nitroaniline (4-NA) with bovine serum albumin (BSA) have been investigated by means of fluorescence spectrometry, synchronous fluorescence spectrometry and UV absorption spectrometry under the simulative physiological conditions. 4-nitroaniline 69-83 albumin Homo sapiens 103-122 17623271-1 2007 The interactions of 2-nitroaniline (2-NA), 3-nitroaniline (3-NA) and 4-nitroaniline (4-NA) with bovine serum albumin (BSA) have been investigated by means of fluorescence spectrometry, synchronous fluorescence spectrometry and UV absorption spectrometry under the simulative physiological conditions. 4-nitroaniline 85-89 albumin Homo sapiens 103-122 17541560-5 2007 The methods are based on the fluorescence enhancement following casein-FITC proteolysis and the visible absorbance of the p-nitroaniline (pNA) produced by BAPNA hydrolysis, with both reactions catalysed by BACE-1. 4-nitroaniline 122-136 beta-secretase 1 Homo sapiens 206-212 17541560-5 2007 The methods are based on the fluorescence enhancement following casein-FITC proteolysis and the visible absorbance of the p-nitroaniline (pNA) produced by BAPNA hydrolysis, with both reactions catalysed by BACE-1. 4-nitroaniline 138-141 beta-secretase 1 Homo sapiens 206-212 19071694-1 2007 The adsorption processes and electrochemical behavior of 4-nitroaniline (4-NA) adsorbed onto glassy carbon electrodes (GCE) have been investigated in aqueous 0.1M nitric acid (HNO(3)) electrolyte solutions using cyclic voltammetry (CV). 4-nitroaniline 57-71 aminomethyltransferase Homo sapiens 119-122 19071694-1 2007 The adsorption processes and electrochemical behavior of 4-nitroaniline (4-NA) adsorbed onto glassy carbon electrodes (GCE) have been investigated in aqueous 0.1M nitric acid (HNO(3)) electrolyte solutions using cyclic voltammetry (CV). 4-nitroaniline 73-77 aminomethyltransferase Homo sapiens 119-122 19071694-2 2007 4-NA adsorbs onto GCE surfaces, and upon potential cycling past -0.2V, is transformed into the arylhydroxylamine (ArHA) derivative which exhibits a well-behaved pH dependent redox couple centered at 0.32V at pH 1.5. 4-nitroaniline 0-4 aminomethyltransferase Homo sapiens 18-21 16526657-0 2006 Structure and dipole moments of the two distinct solvated forms of p-nitroaniline in acetonitrile/CCl4 as studied by infrared electroabsorption spectroscopy. 4-nitroaniline 67-81 C-C motif chemokine ligand 4 Homo sapiens 98-102 16526657-1 2006 Structure and dipole moments of the two distinct solvated forms of p-nitroaniline (pNA) in acetonitrile/CCl4 have been studied by infrared electroabsorption spectroscopy. 4-nitroaniline 67-81 C-C motif chemokine ligand 4 Homo sapiens 104-108 16526657-1 2006 Structure and dipole moments of the two distinct solvated forms of p-nitroaniline (pNA) in acetonitrile/CCl4 have been studied by infrared electroabsorption spectroscopy. 4-nitroaniline 83-86 C-C motif chemokine ligand 4 Homo sapiens 104-108 12214864-4 2002 Kallikrein, a serine protease, catalyzes the splitting of p-nitroaniline (pNA) from its substrate H-D-Pro-Phe-Arg-pNA (S-2302). 4-nitroaniline 58-72 kallikrein related peptidase 4 Homo sapiens 0-10 14982931-2 2004 To explore the biochemical and structural bases for the adenosine phosphoramidate hydrolase activity of rabbit Hint, we synthesized novel substrates linking a p-nitroaniline group to adenylate (AMP-pNA) and inhibitors that consist of an adenosine group and 5"-sulfamoyl (AdoOSO(2)NH(2)) or N-ethylsulfamoyl (AdoOSO(2)NHCH(2)CH(3)) group. 4-nitroaniline 159-173 adenosine 5'-monophosphoramidase HINT1 Oryctolagus cuniculus 111-115 12628481-6 2003 Inhibition of HNE release was measured by p-nitroaniline formation. 4-nitroaniline 42-56 elastase, neutrophil expressed Homo sapiens 14-17 12214864-4 2002 Kallikrein, a serine protease, catalyzes the splitting of p-nitroaniline (pNA) from its substrate H-D-Pro-Phe-Arg-pNA (S-2302). 4-nitroaniline 74-77 kallikrein related peptidase 4 Homo sapiens 0-10 11846431-2 2002 The reactions mainly consisted of formation of a h-TM and h-thrombin complex, activation of h-protein C by the complex and digestion of substrate by activated h-protein C. Linear time-dependent formation of p -nitroaniline from the substrate, S-2366, was observed up to 12 min during measurement of the activity of urinary h-TM (uh-TM) reference material by the standard method. 4-nitroaniline 207-222 coagulation factor II, thrombin Homo sapiens 60-68 10420604-2 1999 Here we demonstrate that MEKC differentiates between the enzymatic activities of Pseudomonas aeruginosa elastase (PsE) and human leukocyte elastase (HLE) or cathepsin G (Cat G) in assays using the chromogenic peptide substrates Suc-Ala-Ala-Ala-NA or Suc-Ala-Ala-Pro-Phe-NA, respectively (where Suc = succinyl and NA = 4-nitroaniline/u-nitroanilide). 4-nitroaniline 318-332 elastase, neutrophil expressed Homo sapiens 129-147 11524009-1 2001 The steady-state kinetics of a full-length and truncated form of the type 2 human methionine aminopeptidase (hMetAP2) were analyzed by continuous monitoring of the amide bond cleavage of various peptide substrates and methionyl analogues of 7-amido-4-methylcoumarin (AMC) and p-nitroaniline (pNA), utilizing new fluorescence-based and absorbance-based assay substrates and a novel coupled-enzyme assay method. 4-nitroaniline 276-290 methionyl aminopeptidase 2 Homo sapiens 109-116 11524009-1 2001 The steady-state kinetics of a full-length and truncated form of the type 2 human methionine aminopeptidase (hMetAP2) were analyzed by continuous monitoring of the amide bond cleavage of various peptide substrates and methionyl analogues of 7-amido-4-methylcoumarin (AMC) and p-nitroaniline (pNA), utilizing new fluorescence-based and absorbance-based assay substrates and a novel coupled-enzyme assay method. 4-nitroaniline 292-295 methionyl aminopeptidase 2 Homo sapiens 109-116 11058845-4 2000 Results for p-nitroaniline are compared with Hartree-Fock (HF), density functional (DFT) and Moller-Plesset (MP2) supermolecular calculations and with HF and DFT periodic calculations. 4-nitroaniline 12-26 tryptase pseudogene 1 Homo sapiens 109-112 11058845-5 2000 The HF and DFT methods fail to predict the stability of the p-nitroaniline crystal but the results of the experimental charge-density approach (ECDA) are in good agreement with both MP2 interaction energies and the experimental lattice energy. 4-nitroaniline 60-74 tryptase pseudogene 1 Homo sapiens 182-185 10576103-8 1999 At input concentrations of 4-NA up to 4.5 mM and a hydraulic retention time of 3.5 hours a high degradation rate of 1.1 mmol 4-NA L-1 h-1 and 90 ... 95% DOC removal were achieved. 4-nitroaniline 27-31 L1 cell adhesion molecule Homo sapiens 130-133 10576103-8 1999 At input concentrations of 4-NA up to 4.5 mM and a hydraulic retention time of 3.5 hours a high degradation rate of 1.1 mmol 4-NA L-1 h-1 and 90 ... 95% DOC removal were achieved. 4-nitroaniline 125-129 L1 cell adhesion molecule Homo sapiens 130-133 10441360-7 1999 Addition of partially denatured cytochrome c (most likely in the molten globule state) to the mixture of enzyme- and substrate-containing liposomes resulted in the release of one of the hydrolysis products, p-nitroaniline, to the outer phase of the fused liposomes, indicating that the enzymatic reaction occurred during the liposome fusion process. 4-nitroaniline 207-221 cytochrome c, somatic Homo sapiens 32-44 9882013-5 1998 Mutagenicity and/or cytotoxicity of these gamma-glutamyl derivatives were restored upon reaction with GGT, with concomitant release of PH, and p-nitroaniline (PNA). 4-nitroaniline 143-157 inactive glutathione hydrolase 2 Homo sapiens 102-105 9795931-3 1998 Kallikrein was estimated by means of the cleavage of the chromogenic tripeptide Val-Leu-Arg-p-nitranilide (SIGMA) and absorbance measuring of p-nitraniline at 405 nm. 4-nitroaniline 142-155 kallikrein related peptidase 4 Homo sapiens 0-10 9882013-5 1998 Mutagenicity and/or cytotoxicity of these gamma-glutamyl derivatives were restored upon reaction with GGT, with concomitant release of PH, and p-nitroaniline (PNA). 4-nitroaniline 159-162 inactive glutathione hydrolase 2 Homo sapiens 102-105 7714663-2 1995 Its hydrolysis by DPPIV resulted in the formation of a chromophore, 2,6-dibromophenol-indo-p-xylenol, and its maximal absorption wavelength (600 nm) was longer than that of p-nitroaniline (415 nm) released from conventional substrate, glycyl-L-proline p-nitroanilide (Gly-Pro-pNA). 4-nitroaniline 173-187 dipeptidyl peptidase 4 Homo sapiens 18-23 9047311-7 1997 Substrates which contain an ANSN leaving group had a higher affinity for tPA than substrates with p-nitroaniline or 7-amino-4-methylcoumarin leaving groups. 4-nitroaniline 98-112 plasminogen activator, tissue type Homo sapiens 73-76 7509511-1 1993 A method is described by which the time-course of thrombin generation in plasma can be obtained from a continuous optical density recording of p-nitroaniline (pNA) production in a 2:3 diluted plasma. 4-nitroaniline 143-157 coagulation factor II, thrombin Homo sapiens 50-58 7509511-1 1993 A method is described by which the time-course of thrombin generation in plasma can be obtained from a continuous optical density recording of p-nitroaniline (pNA) production in a 2:3 diluted plasma. 4-nitroaniline 159-162 coagulation factor II, thrombin Homo sapiens 50-58 8251746-2 1993 Leukotriene A4 hydrolase (LTA4H) is an epoxide hydrolase, catalyzing the hydration of LTA4 to LTB4, and also acts an aminopeptidase, with the ability to cleave amides of p-nitroaniline. 4-nitroaniline 170-184 leukotriene A4 hydrolase Homo sapiens 0-24 8251746-2 1993 Leukotriene A4 hydrolase (LTA4H) is an epoxide hydrolase, catalyzing the hydration of LTA4 to LTB4, and also acts an aminopeptidase, with the ability to cleave amides of p-nitroaniline. 4-nitroaniline 170-184 leukotriene A4 hydrolase Homo sapiens 26-31 1975183-1 1990 The localization of gamma-glutamyl transferase (GGT) in the intact rat liver was studied by a new approach in which the chromogenic gamma-glutamyl donor substrate of GGT gamma-glutamyl-p-nitroanilide is perfused through the portal vein to yield p-nitroaniline, which is monitored spectrophotometrically. 4-nitroaniline 245-259 gamma-glutamyltransferase 1 Rattus norvegicus 20-46 8280677-4 1993 Kallikrein is easily detected by its reaction with the chromogenic substrate H-D-Pro-Phe-Arg-pNA, which releases p-nitroaniline, revealed by its absorption at 405 nm. 4-nitroaniline 113-127 kallikrein related peptidase 4 Homo sapiens 0-10 1505501-2 1992 The method is based on the enzymatic release of p-nitroaniline (PNA) from two specific synthetic oxytocinase substrates, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN), respectively. 4-nitroaniline 48-62 leucyl and cystinyl aminopeptidase Homo sapiens 97-108 1505501-2 1992 The method is based on the enzymatic release of p-nitroaniline (PNA) from two specific synthetic oxytocinase substrates, S-benzyl-L-cysteine-p-nitroanilide (BCN) and L-leucine-p-nitroanilide (LN), respectively. 4-nitroaniline 64-67 leucyl and cystinyl aminopeptidase Homo sapiens 97-108 18965228-2 1991 It is based on the hydrolysis of a synthetic chromogenic substrate during the stopped-flow period, yielding p-nitroaniline, which is then preconcentrated on a C-18 column. 4-nitroaniline 108-122 Bardet-Biedl syndrome 9 Homo sapiens 159-163 1975183-1 1990 The localization of gamma-glutamyl transferase (GGT) in the intact rat liver was studied by a new approach in which the chromogenic gamma-glutamyl donor substrate of GGT gamma-glutamyl-p-nitroanilide is perfused through the portal vein to yield p-nitroaniline, which is monitored spectrophotometrically. 4-nitroaniline 245-259 gamma-glutamyltransferase 1 Rattus norvegicus 48-51 1975183-1 1990 The localization of gamma-glutamyl transferase (GGT) in the intact rat liver was studied by a new approach in which the chromogenic gamma-glutamyl donor substrate of GGT gamma-glutamyl-p-nitroanilide is perfused through the portal vein to yield p-nitroaniline, which is monitored spectrophotometrically. 4-nitroaniline 245-259 gamma-glutamyltransferase 1 Homo sapiens 166-169 1975183-3 1990 Infusion of glutathione (GSH), the natural substrate of GGT, was shown to markedly reduce or to abolish the formation of p-nitroaniline without entering the liver cells, indicating the existence of a GGT ectoactivity accessible to the sinusoidal circulation. 4-nitroaniline 121-135 gamma-glutamyltransferase 1 Rattus norvegicus 56-59 1975183-3 1990 Infusion of glutathione (GSH), the natural substrate of GGT, was shown to markedly reduce or to abolish the formation of p-nitroaniline without entering the liver cells, indicating the existence of a GGT ectoactivity accessible to the sinusoidal circulation. 4-nitroaniline 121-135 gamma-glutamyltransferase 1 Rattus norvegicus 200-203 1968221-9 1990 gamma-Glutamyl transpeptidase (EC 2.3.2.2) activity was 5-fold elevated in H69AR cells, compared with H69 cells (2.50 +/- 0.44 versus 0.46 +/- 0.21 nmol of p-nitroaniline/min/mg of protein) (p less than 0.01), whereas GSH-S-transferase (EC 2.5.1.18) activity was 10-fold higher (201.98 +/- 43.62 versus 19.77 +/- 1.72 nmol of 1-chloro-2,4-dinitrobenzene/min/mg of protein in H69AR and H69 cells, respectively) (p less than 0.01). 4-nitroaniline 156-170 inactive glutathione hydrolase 2 Homo sapiens 0-29 34771354-6 2021 Following the pseudo-first-order kinetics, 5 mg of the catalyst led to a 90% conversion of 4-NP with the mass-normalized rate constant (km1) of 6.94 x 10-3 min-1 mg-1, while the corresponding value acquired for 4-NA was 7.2 x 10-3 min-1 mg-1, despite the trace amount of Re in the heterogenous catalyst. 4-nitroaniline 211-215 CD59 molecule (CD59 blood group) Homo sapiens 231-241