PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
9925781-8	1999	Furthermore, the scFv fragment, but not the Fv fragment, gives rise to premature interface formation, indicated by the fluorescence spectra and a much higher transient binding of 8-anilino-1-naphthalene sulfonate.	8-anilino-1-naphthalenesulfonic acid	179-212	immunglobulin heavy chain variable region	Homo sapiens	17-21
9914513-3	1999	In addition, ATP induces a conformational change in GroEL characterized by (a) the appearance of a reversible low temperature endotherm in the DSC profiles of the protein, and (b) an enhanced binding of the hydrophobic probe 8-anilino-naphthalene-1-sulfonate (ANS), which strictly depends on ATP hydrolysis.	8-anilino-1-naphthalenesulfonic acid	225-258	heat shock protein family D (Hsp60) member 1	Homo sapiens	52-57
9914513-3	1999	In addition, ATP induces a conformational change in GroEL characterized by (a) the appearance of a reversible low temperature endotherm in the DSC profiles of the protein, and (b) an enhanced binding of the hydrophobic probe 8-anilino-naphthalene-1-sulfonate (ANS), which strictly depends on ATP hydrolysis.	8-anilino-1-naphthalenesulfonic acid	260-263	heat shock protein family D (Hsp60) member 1	Homo sapiens	52-57
9914513-4	1999	The similar sensitivity to K+ of the temperature range where activation of the GroEL ATPase activity, the low temperature endotherm, and the increase of the ANS fluorescence are abserved strongly indicates the existence of a conformational state of GroEL during ATP hydrolysis, different from that generated on ADP or AMP-PNP binding.	8-anilino-1-naphthalenesulfonic acid	157-160	heat shock protein family D (Hsp60) member 1	Homo sapiens	249-254
9988521-6	1998	The changes in CRP structure induced by GuHCl were monitored using both intrinsic Trp fluorescence as well as the fluorescence of an extrinsic probe, 8-anilino-1-Naphthalenesulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	150-186	catabolite gene activator protein	Escherichia coli	15-18
10410396-3	1999	The dependence of spectral properties of Mg2+ and NH4+ salt of 8-anilino-1-naphthalenesulfonic acid (Mg-(ANS)2 and NH4-ANS, respectively) on the dye concentration and solvent composition was investigated by means of steady-state and time-resolved fluorescence spectroscopy.	8-anilino-1-naphthalenesulfonic acid	63-99	mucin 7, secreted	Homo sapiens	41-44
9799517-1	1998	Solvent-induced equilibrium unfolding of a homodimeric class sigma glutathione transferase (GSTS1-1, EC 2.5.1.18) was characterized by tryptophan fluorescence, anisotropy, enzyme activity, 8-anilino-1-naphthalenesulfonate (ANS) binding, and circular dichroism.	8-anilino-1-naphthalenesulfonic acid	189-221	hematopoietic prostaglandin D synthase	Homo sapiens	92-99
9988521-6	1998	The changes in CRP structure induced by GuHCl were monitored using both intrinsic Trp fluorescence as well as the fluorescence of an extrinsic probe, 8-anilino-1-Naphthalenesulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	188-191	catabolite gene activator protein	Escherichia coli	15-18
9988521-10	1998	The denaturation of CRP monitored by ANS fluorescence reveals the existence of two additional processes.	8-anilino-1-naphthalenesulfonic acid	37-40	catabolite gene activator protein	Escherichia coli	20-23
8168536-8	1994	Both kinetic measurements and fluorescence measurements of PLA2-bound 8-anilino-1-naphthalene sulfonate in the presence of varying amounts of heparin showed that a heparin molecule bound about seven molecules of PLA2.	8-anilino-1-naphthalenesulfonic acid	70-103	phospholipase A2 group IIA	Homo sapiens	59-63
7696312-1	1995	The equilibrium binding of the non-substrate ligands 8-anilino-1-naphthalene sulfonate and bromosulfophthalein to porcine class pi glutathione S-transferase (pGSTP1-1) was studied using a variety of techniques (size-exclusion HPLC, steady-state fluorescence, second-derivative spectroscopy, and chemical modification of cysteines).	8-anilino-1-naphthalenesulfonic acid	53-86	glutathione S-transferase kappa 1	Homo sapiens	131-156
8043585-0	1994	Ligand interaction between urokinase-type plasminogen activator and its receptor probed with 8-anilino-1-naphthalenesulfonate.	8-anilino-1-naphthalenesulfonic acid	93-125	plasminogen activator, urokinase	Homo sapiens	27-63
8043585-4	1994	Here we report that intact uPAR binds the low molecular weight fluorophore 8-anilino-1-naphthalenesulfonate (ANS) to form a 1:1 stoichiometric complex and that the resulting enhancement of the ANS fluorescence probes the functional state of uPAR as judged by several independent criteria.	8-anilino-1-naphthalenesulfonic acid	75-107	plasminogen activator, urokinase receptor	Homo sapiens	27-31
8043585-4	1994	Here we report that intact uPAR binds the low molecular weight fluorophore 8-anilino-1-naphthalenesulfonate (ANS) to form a 1:1 stoichiometric complex and that the resulting enhancement of the ANS fluorescence probes the functional state of uPAR as judged by several independent criteria.	8-anilino-1-naphthalenesulfonic acid	75-107	plasminogen activator, urokinase receptor	Homo sapiens	241-245
8043585-4	1994	Here we report that intact uPAR binds the low molecular weight fluorophore 8-anilino-1-naphthalenesulfonate (ANS) to form a 1:1 stoichiometric complex and that the resulting enhancement of the ANS fluorescence probes the functional state of uPAR as judged by several independent criteria.	8-anilino-1-naphthalenesulfonic acid	109-112	plasminogen activator, urokinase receptor	Homo sapiens	27-31
8043585-4	1994	Here we report that intact uPAR binds the low molecular weight fluorophore 8-anilino-1-naphthalenesulfonate (ANS) to form a 1:1 stoichiometric complex and that the resulting enhancement of the ANS fluorescence probes the functional state of uPAR as judged by several independent criteria.	8-anilino-1-naphthalenesulfonic acid	109-112	plasminogen activator, urokinase receptor	Homo sapiens	241-245
8043585-4	1994	Here we report that intact uPAR binds the low molecular weight fluorophore 8-anilino-1-naphthalenesulfonate (ANS) to form a 1:1 stoichiometric complex and that the resulting enhancement of the ANS fluorescence probes the functional state of uPAR as judged by several independent criteria.	8-anilino-1-naphthalenesulfonic acid	193-196	plasminogen activator, urokinase receptor	Homo sapiens	27-31
8043585-4	1994	Here we report that intact uPAR binds the low molecular weight fluorophore 8-anilino-1-naphthalenesulfonate (ANS) to form a 1:1 stoichiometric complex and that the resulting enhancement of the ANS fluorescence probes the functional state of uPAR as judged by several independent criteria.	8-anilino-1-naphthalenesulfonic acid	193-196	plasminogen activator, urokinase receptor	Homo sapiens	241-245
8043585-5	1994	First, the uPAR-mediated increase in ANS fluorescence can be titrated by uPA as well as by its receptor binding derivatives (the amino-terminal fragment and the growth factor-like module).	8-anilino-1-naphthalenesulfonic acid	37-40	plasminogen activator, urokinase receptor	Homo sapiens	11-15
8043585-5	1994	First, the uPAR-mediated increase in ANS fluorescence can be titrated by uPA as well as by its receptor binding derivatives (the amino-terminal fragment and the growth factor-like module).	8-anilino-1-naphthalenesulfonic acid	37-40	plasminogen activator, urokinase	Homo sapiens	11-14
8043585-6	1994	Second, an anti-uPAR monoclonal antibody, capable of preventing uPA binding, can also titrate the uPAR-dependent ANS fluorescence whereas other antibodies not interfering with uPA binding are unable to exert this effect.	8-anilino-1-naphthalenesulfonic acid	113-116	plasminogen activator, urokinase receptor	Homo sapiens	16-20
8043585-6	1994	Second, an anti-uPAR monoclonal antibody, capable of preventing uPA binding, can also titrate the uPAR-dependent ANS fluorescence whereas other antibodies not interfering with uPA binding are unable to exert this effect.	8-anilino-1-naphthalenesulfonic acid	113-116	plasminogen activator, urokinase	Homo sapiens	16-19
8043585-6	1994	Second, an anti-uPAR monoclonal antibody, capable of preventing uPA binding, can also titrate the uPAR-dependent ANS fluorescence whereas other antibodies not interfering with uPA binding are unable to exert this effect.	8-anilino-1-naphthalenesulfonic acid	113-116	plasminogen activator, urokinase receptor	Homo sapiens	98-102
8043585-6	1994	Second, an anti-uPAR monoclonal antibody, capable of preventing uPA binding, can also titrate the uPAR-dependent ANS fluorescence whereas other antibodies not interfering with uPA binding are unable to exert this effect.	8-anilino-1-naphthalenesulfonic acid	113-116	plasminogen activator, urokinase	Homo sapiens	64-67
8043585-7	1994	Third, the dissociation profile of uPA-uPAR complexes as a function of increasing concentrations of guanidine hydrochloride closely parallels the loss of the ANS binding site in uPAR.	8-anilino-1-naphthalenesulfonic acid	158-161	plasminogen activator, urokinase	Homo sapiens	35-38
8043585-7	1994	Third, the dissociation profile of uPA-uPAR complexes as a function of increasing concentrations of guanidine hydrochloride closely parallels the loss of the ANS binding site in uPAR.	8-anilino-1-naphthalenesulfonic acid	158-161	plasminogen activator, urokinase receptor	Homo sapiens	39-43
8043585-7	1994	Third, the dissociation profile of uPA-uPAR complexes as a function of increasing concentrations of guanidine hydrochloride closely parallels the loss of the ANS binding site in uPAR.	8-anilino-1-naphthalenesulfonic acid	158-161	plasminogen activator, urokinase receptor	Homo sapiens	178-182
8043585-8	1994	Finally, liberation of the NH2-terminal domain from uPAR by limited chymotrypsin cleavage after Tyr87 leads to a loss of both enhanced ANS fluorescence and high-affinity uPA binding.	8-anilino-1-naphthalenesulfonic acid	135-138	plasminogen activator, urokinase receptor	Homo sapiens	52-56
8043585-8	1994	Finally, liberation of the NH2-terminal domain from uPAR by limited chymotrypsin cleavage after Tyr87 leads to a loss of both enhanced ANS fluorescence and high-affinity uPA binding.	8-anilino-1-naphthalenesulfonic acid	135-138	plasminogen activator, urokinase	Homo sapiens	52-55
7798180-11	1994	The aggregation of myosin molecules upon pressurization was concomitant with an increase in hydrophobicity, which was measured spectrofluorometrically with 8-anilino-1-naphthalene sulfonate, a probe for apolar binding sites.	8-anilino-1-naphthalenesulfonic acid	156-189	myosin heavy chain 14	Homo sapiens	19-25
9054543-9	1997	DTT-IL-6 appears to form a partially unfolded and highly aggregated conformation under all conditions studied, as showed by a high propensity to self-associate (demonstrated using a biosensor employing surface plasmon resonance), and an increased ability to bind the hydrophobic probe 8-anilino-1-naphthalenesulfonic acid.	8-anilino-1-naphthalenesulfonic acid	285-321	interleukin 6	Mus musculus	4-8
8706705-6	1996	There was an increase in the fluorescence intensity of a hydrophobic fluorescent probe, 8-anilino-1-naphthalenesulfonate, in the presence of MBP-CD38.	8-anilino-1-naphthalenesulfonic acid	88-120	myelin basic protein	Homo sapiens	141-144
8706705-6	1996	There was an increase in the fluorescence intensity of a hydrophobic fluorescent probe, 8-anilino-1-naphthalenesulfonate, in the presence of MBP-CD38.	8-anilino-1-naphthalenesulfonic acid	88-120	CD38 molecule	Homo sapiens	145-149
8563628-4	1995	In the present study, CPL measurements were applied using the intrinsic tryptophan fluorescence of alpha-LA as well as the fluorescence of 8-anilino-1-naphthalenesulfonic acid (ANS) bound to alpha-LA.	8-anilino-1-naphthalenesulfonic acid	139-175	lactalbumin alpha	Bos taurus	191-199
8563628-4	1995	In the present study, CPL measurements were applied using the intrinsic tryptophan fluorescence of alpha-LA as well as the fluorescence of 8-anilino-1-naphthalenesulfonic acid (ANS) bound to alpha-LA.	8-anilino-1-naphthalenesulfonic acid	177-180	lactalbumin alpha	Bos taurus	191-199
8563628-8	1995	This transition was interpreted as being a result of a change of the alpha-LA tertiary structure, which resulted in a loss of asymmetry of the environment of both the tryptophan residues and the ANS hydrophobic binding sites.	8-anilino-1-naphthalenesulfonic acid	195-198	lactalbumin alpha	Bos taurus	69-77
7548000-7	1995	Modification of uPAR alone led to a parallel reduction in the potential to bind pro-uPA and 8-anilino-1-naphthalenesulfonate, an extrinsic fluorophore reporting on the accessibility of a hydrophobic site involved in uPA binding.	8-anilino-1-naphthalenesulfonic acid	92-124	plasminogen activator, urokinase receptor	Homo sapiens	16-20
7548000-7	1995	Modification of uPAR alone led to a parallel reduction in the potential to bind pro-uPA and 8-anilino-1-naphthalenesulfonate, an extrinsic fluorophore reporting on the accessibility of a hydrophobic site involved in uPA binding.	8-anilino-1-naphthalenesulfonic acid	92-124	plasminogen activator, urokinase	Homo sapiens	16-19
7702745-1	1994	The binding of the apolar fluorescent dye 8-anilinonaphthalene-1-sulfonate (ANS) to bovine serum albumin (BSA), phospholipase A2 (PLA2), ovalbumin, lysozyme, cobrotoxin and N-acetyltryptophanamide was used to assess the factors affecting the efficiency of energy transfer from Trp residues to the ANS molecule.	8-anilino-1-naphthalenesulfonic acid	42-74	albumin	Homo sapiens	91-104
7702745-1	1994	The binding of the apolar fluorescent dye 8-anilinonaphthalene-1-sulfonate (ANS) to bovine serum albumin (BSA), phospholipase A2 (PLA2), ovalbumin, lysozyme, cobrotoxin and N-acetyltryptophanamide was used to assess the factors affecting the efficiency of energy transfer from Trp residues to the ANS molecule.	8-anilino-1-naphthalenesulfonic acid	42-74	phospholipase A2 group IB	Homo sapiens	130-134
7702745-1	1994	The binding of the apolar fluorescent dye 8-anilinonaphthalene-1-sulfonate (ANS) to bovine serum albumin (BSA), phospholipase A2 (PLA2), ovalbumin, lysozyme, cobrotoxin and N-acetyltryptophanamide was used to assess the factors affecting the efficiency of energy transfer from Trp residues to the ANS molecule.	8-anilino-1-naphthalenesulfonic acid	76-79	albumin	Homo sapiens	91-104
7702745-1	1994	The binding of the apolar fluorescent dye 8-anilinonaphthalene-1-sulfonate (ANS) to bovine serum albumin (BSA), phospholipase A2 (PLA2), ovalbumin, lysozyme, cobrotoxin and N-acetyltryptophanamide was used to assess the factors affecting the efficiency of energy transfer from Trp residues to the ANS molecule.	8-anilino-1-naphthalenesulfonic acid	76-79	phospholipase A2 group IB	Homo sapiens	112-128
7702745-4	1994	Fluorescence enhancement of ANS in PLA2-ANS complex increased upon addition of Ca2+ or change of the buffer to acidic pH, resulting in a higher efficiency of energy transfer from Trp residues to ANS.	8-anilino-1-naphthalenesulfonic acid	28-31	phospholipase A2 group IB	Homo sapiens	35-39
7702745-4	1994	Fluorescence enhancement of ANS in PLA2-ANS complex increased upon addition of Ca2+ or change of the buffer to acidic pH, resulting in a higher efficiency of energy transfer from Trp residues to ANS.	8-anilino-1-naphthalenesulfonic acid	40-43	phospholipase A2 group IB	Homo sapiens	35-39
7702745-4	1994	Fluorescence enhancement of ANS in PLA2-ANS complex increased upon addition of Ca2+ or change of the buffer to acidic pH, resulting in a higher efficiency of energy transfer from Trp residues to ANS.	8-anilino-1-naphthalenesulfonic acid	40-43	phospholipase A2 group IB	Homo sapiens	35-39
7702745-5	1994	There was limited ANS fluorescence enhancement with ovalbumin, lysozyme, cobrotoxin, and N-acetyltryptophanamide and a less efficient quenching in Trp fluorescence.	8-anilino-1-naphthalenesulfonic acid	18-21	lysozyme	Homo sapiens	63-71
8168536-8	1994	Both kinetic measurements and fluorescence measurements of PLA2-bound 8-anilino-1-naphthalene sulfonate in the presence of varying amounts of heparin showed that a heparin molecule bound about seven molecules of PLA2.	8-anilino-1-naphthalenesulfonic acid	70-103	phospholipase A2 group IIA	Homo sapiens	212-216
34838360-5	2022	Site-specific HSA binding fluorophores, 8-anilinonaphthalene-1-sulfonic acid (1,8-ANS), warfarin and dansyl-L-proline were used to investigate the specific binding sites of Gen X on HSA.	8-anilino-1-naphthalenesulfonic acid	40-76	GEN1 Holliday junction 5' flap endonuclease	Homo sapiens	173-176
2161434-7	1990	8-Anilino-1-naphthalene sulfonic acid bound to calmodulin, studied spectrofluorometrically, was not displaced by retinoic acid.	8-anilino-1-naphthalenesulfonic acid	0-37	calmodulin 1	Homo sapiens	47-57
8280758-8	1994	8-Anilinonaphthalene-1-sulfonate (ANS), a strongly fluorescing dye, binds to the thyroxine binding sites on TTR.	8-anilino-1-naphthalenesulfonic acid	0-32	transthyretin	Homo sapiens	108-111
8280758-8	1994	8-Anilinonaphthalene-1-sulfonate (ANS), a strongly fluorescing dye, binds to the thyroxine binding sites on TTR.	8-anilino-1-naphthalenesulfonic acid	34-37	transthyretin	Homo sapiens	108-111
8280758-9	1994	T4 and 3,5,3"-L-triiodothyronine (T3) shifted the fluorescence emission maximum and intensity of an ANS-TTR solution toward the spectrum obtained from uncomplexed ANS.	8-anilino-1-naphthalenesulfonic acid	100-103	transthyretin	Homo sapiens	104-107
8280758-9	1994	T4 and 3,5,3"-L-triiodothyronine (T3) shifted the fluorescence emission maximum and intensity of an ANS-TTR solution toward the spectrum obtained from uncomplexed ANS.	8-anilino-1-naphthalenesulfonic acid	163-166	transthyretin	Homo sapiens	104-107
8280758-11	1994	Retinol failed to quench the emission intensity of the ANS-TTR complex, while 13-cis-retinoic acid was less effective than all-trans retinoic acid.	8-anilino-1-naphthalenesulfonic acid	55-58	transthyretin	Homo sapiens	59-62
34080430-10	2021	By using intrinsic and 8-anilino-1-naphthalene sulfonate fluorescence titration experiments, we found that POMs bind to S100A9 with a Kd of ca.	8-anilino-1-naphthalenesulfonic acid	23-56	S100 calcium binding protein A9	Homo sapiens	120-126
34151917-5	2021	The PI gel with the ANS monomers using bovine serum albumin (BSA) as a template showed the selective adsorption of BSA and the fluorescence intensity increased due to the adsorption of BSA.	8-anilino-1-naphthalenesulfonic acid	20-23	albumin	Homo sapiens	46-59
34356542-3	2021	We investigated the binding of the small ligand 8-anilinonaphthalene-1-sulfonic acid (ANS) to the multifunctional protein bovine serum albumin (BSA) at ambient and low temperatures and at high pressure conditions, in the presence of ions associated with the surface and subsurface of Mars, including the chaotropic perchlorate ion.	8-anilino-1-naphthalenesulfonic acid	48-84	albumin	Homo sapiens	129-142
34356542-3	2021	We investigated the binding of the small ligand 8-anilinonaphthalene-1-sulfonic acid (ANS) to the multifunctional protein bovine serum albumin (BSA) at ambient and low temperatures and at high pressure conditions, in the presence of ions associated with the surface and subsurface of Mars, including the chaotropic perchlorate ion.	8-anilino-1-naphthalenesulfonic acid	86-89	albumin	Homo sapiens	129-142
35282732-3	2022	Cofactor activity (CA) was measured using 8-anilinonaphthalene-1-sulfonic acid as a fluorescent probe of C3b integrity.	8-anilino-1-naphthalenesulfonic acid	42-78	complement C3	Homo sapiens	105-108
2611263-7	1989	In the presence of PMPS- or DEP-modified TFIIIA or Zn2+-depleted TFIIIA, the fluorescence emission maximum of the hydrophobic probe, 8-anilinonaphthalenesulfonate, was blue-shifted by 30 nm, while only less than a 10-nm blue shift was observed in the presence of either the 7S particle or TFIIIA.	8-anilino-1-naphthalenesulfonic acid	133-162	general transcription factor 3A L homeolog	Xenopus laevis	65-71
2611263-7	1989	In the presence of PMPS- or DEP-modified TFIIIA or Zn2+-depleted TFIIIA, the fluorescence emission maximum of the hydrophobic probe, 8-anilinonaphthalenesulfonate, was blue-shifted by 30 nm, while only less than a 10-nm blue shift was observed in the presence of either the 7S particle or TFIIIA.	8-anilino-1-naphthalenesulfonic acid	133-162	general transcription factor 3A L homeolog	Xenopus laevis	65-71
6654860-5	1983	The fluorescence of the complex of CRP and 8-anilino-1-naphthalene sulfonate (ANS) changed with change of the pH, suggesting that CRP may show pH-dependent conformational change.	8-anilino-1-naphthalenesulfonic acid	43-76	C-reactive protein	Homo sapiens	35-38
2611263-7	1989	In the presence of PMPS- or DEP-modified TFIIIA or Zn2+-depleted TFIIIA, the fluorescence emission maximum of the hydrophobic probe, 8-anilinonaphthalenesulfonate, was blue-shifted by 30 nm, while only less than a 10-nm blue shift was observed in the presence of either the 7S particle or TFIIIA.	8-anilino-1-naphthalenesulfonic acid	133-162	general transcription factor 3A L homeolog	Xenopus laevis	41-47
3385211-7	1988	The modification of the CRP structure was confirmed by the change in the fluorescence spectrum of 8-anilino-1-naphthalene sulfonate complexed with CRP, the increased susceptibility of CRP to proteolytic enzymes and the altered reactivity to anti-CRP mAb.	8-anilino-1-naphthalenesulfonic acid	98-131	C-reactive protein	Homo sapiens	24-27
3385211-7	1988	The modification of the CRP structure was confirmed by the change in the fluorescence spectrum of 8-anilino-1-naphthalene sulfonate complexed with CRP, the increased susceptibility of CRP to proteolytic enzymes and the altered reactivity to anti-CRP mAb.	8-anilino-1-naphthalenesulfonic acid	98-131	C-reactive protein	Homo sapiens	147-150
3385211-7	1988	The modification of the CRP structure was confirmed by the change in the fluorescence spectrum of 8-anilino-1-naphthalene sulfonate complexed with CRP, the increased susceptibility of CRP to proteolytic enzymes and the altered reactivity to anti-CRP mAb.	8-anilino-1-naphthalenesulfonic acid	98-131	C-reactive protein	Homo sapiens	147-150
3385211-7	1988	The modification of the CRP structure was confirmed by the change in the fluorescence spectrum of 8-anilino-1-naphthalene sulfonate complexed with CRP, the increased susceptibility of CRP to proteolytic enzymes and the altered reactivity to anti-CRP mAb.	8-anilino-1-naphthalenesulfonic acid	98-131	C-reactive protein	Homo sapiens	147-150
3338992-3	1988	The extrinsic fluorescent probe 8-anilino-1-naphthalene sulfonate (ANS) and factor B were found to act as competitive ligands in binding to C3b.	8-anilino-1-naphthalenesulfonic acid	32-65	complement C3	Homo sapiens	140-143
3338992-3	1988	The extrinsic fluorescent probe 8-anilino-1-naphthalene sulfonate (ANS) and factor B were found to act as competitive ligands in binding to C3b.	8-anilino-1-naphthalenesulfonic acid	67-70	complement C3	Homo sapiens	140-143
3338992-4	1988	Thus, complex formation between C3b or C3(H2O) and factor B could be monitored by the quenching of C3b/C3(H2O)-dependent ANS fluorescence upon the addition of B.	8-anilino-1-naphthalenesulfonic acid	121-124	complement C3	Homo sapiens	32-35
3338992-4	1988	Thus, complex formation between C3b or C3(H2O) and factor B could be monitored by the quenching of C3b/C3(H2O)-dependent ANS fluorescence upon the addition of B.	8-anilino-1-naphthalenesulfonic acid	121-124	complement C3	Homo sapiens	39-46
3338992-4	1988	Thus, complex formation between C3b or C3(H2O) and factor B could be monitored by the quenching of C3b/C3(H2O)-dependent ANS fluorescence upon the addition of B.	8-anilino-1-naphthalenesulfonic acid	121-124	complement C3	Homo sapiens	99-102
3338992-4	1988	Thus, complex formation between C3b or C3(H2O) and factor B could be monitored by the quenching of C3b/C3(H2O)-dependent ANS fluorescence upon the addition of B.	8-anilino-1-naphthalenesulfonic acid	121-124	complement C3	Homo sapiens	103-110
3948999-3	1986	8-Anilino-1-naphthalenesulfonate fluorescence, pyrene excimer fluorescence and diphenylhexatriene fluorescence polarisation studies indicate that seminalplasmin binds to the spermatozoal membranes, and leads to an increase in the fluidity of both the plasma and the acrosomal membranes.	8-anilino-1-naphthalenesulfonic acid	0-32	caltrin	Bos taurus	146-160
6333249-4	1984	In addition, the quantum yield of intrinsic (tryptophan/tyrosine) fluorescence decreased by 33% after reduction and alkylation, and the affinity of the hydrophobic fluorescent probes 8-anilino-1-naphthalenesulfonate and 6-(p-toluidino)-2-naphthalenesulfonate for vWF was reduced 2.5-fold.	8-anilino-1-naphthalenesulfonic acid	183-215	von Willebrand factor	Homo sapiens	263-266
2583190-0	1989	Ligand-induced conformational changes of citrate synthase studied with the fluorescent probe 8-anilinonaphthalene 1-sulfonate.	8-anilino-1-naphthalenesulfonic acid	93-125	citrate synthase	Homo sapiens	41-57
2583190-1	1989	Experiments are presented which show that oxaloacetate and analogs thereof with (R)-malate substructure, on interaction with citrate synthase linked to synthase 8-anilinonaphthalene 1-sulfonate (ANS), induce identical conformational changes of a characteristic magnitude.	8-anilino-1-naphthalenesulfonic acid	161-193	citrate synthase	Homo sapiens	125-141
2583190-1	1989	Experiments are presented which show that oxaloacetate and analogs thereof with (R)-malate substructure, on interaction with citrate synthase linked to synthase 8-anilinonaphthalene 1-sulfonate (ANS), induce identical conformational changes of a characteristic magnitude.	8-anilino-1-naphthalenesulfonic acid	195-198	citrate synthase	Homo sapiens	125-141
2583190-2	1989	A conformational change of lower magnitude is also produced on binding of CoASH or ATP to citrate synthase.ANS and is completed on addition of oxaloacetate.	8-anilino-1-naphthalenesulfonic acid	107-110	citrate synthase	Homo sapiens	90-106
6654860-5	1983	The fluorescence of the complex of CRP and 8-anilino-1-naphthalene sulfonate (ANS) changed with change of the pH, suggesting that CRP may show pH-dependent conformational change.	8-anilino-1-naphthalenesulfonic acid	43-76	C-reactive protein	Homo sapiens	130-133
6654860-5	1983	The fluorescence of the complex of CRP and 8-anilino-1-naphthalene sulfonate (ANS) changed with change of the pH, suggesting that CRP may show pH-dependent conformational change.	8-anilino-1-naphthalenesulfonic acid	78-81	C-reactive protein	Homo sapiens	35-38
6654860-5	1983	The fluorescence of the complex of CRP and 8-anilino-1-naphthalene sulfonate (ANS) changed with change of the pH, suggesting that CRP may show pH-dependent conformational change.	8-anilino-1-naphthalenesulfonic acid	78-81	C-reactive protein	Homo sapiens	130-133
6654860-7	1983	Ca2+-dependent change of the fluorescence of the complex of CRP and ANS was also detected.	8-anilino-1-naphthalenesulfonic acid	68-71	C-reactive protein	Homo sapiens	60-63
6220003-1	1983	The conformational basis for the loss of C3b functional site expression following its conversion to iC3b by the regulatory proteins factor H and factor I has been examined by a number of spectroscopic techniques including the fluorescence of the extrinsic probe 8-anilino-1-naphthalenesulfonate, circular dichroism, and UV absorption difference spectroscopy.	8-anilino-1-naphthalenesulfonic acid	262-294	complement C3	Homo sapiens	41-44
6220003-5	1983	The most dramatic effect was seen in the 8-anilino-1-naphthalenesulfonate fluorescence system where the fluorescence enhancement observed upon conversion of C3 to C3b, which presumably reflects an increase in surface hydrophobicity, is lost upon further cleavage of the molecule to iC3b.	8-anilino-1-naphthalenesulfonic acid	41-73	complement C3	Homo sapiens	163-166
6897780-7	1982	The bindings of 125I-T3 and of 125I-T4 to sera in the presence of 8-anilino-1-naphthalene sulfonic acid to block binding to TBG (non-treated sera) were markedly higher in the two patients before therapy than those in ten normal controls (11.8% and 52.3% in Case 1, 46.8% and 21.5% in Case 2, and 5.9 +/- 0.6% and 4.1 +/- 0.5% (mean +/- SD) in the controls, respectively).	8-anilino-1-naphthalenesulfonic acid	66-103	serpin family A member 7	Homo sapiens	124-127
6822544-7	1983	Furthermore, the calmodulin-induced increase in phosphorylation, but not that produced by phospholipids, was blocked by 8-anilino-1-naphthalenesulfonate.	8-anilino-1-naphthalenesulfonic acid	120-152	calmodulin 1	Homo sapiens	17-27
6250577-7	1980	Direct binding studies using equilibrium dialysis demonstrated that CaM can bind four to six molecules of 9AC or two to three molecules of Ans in a calcium-dependent manner.	8-anilino-1-naphthalenesulfonic acid	139-142	calmodulin 1	Homo sapiens	68-71
6282830-5	1982	The deglycosylated hCG was stable in the lyophilized form and retained its quaternary structure as revealed by the fluorescence probe 8-anilino 1-naphthalene sulfonic acid, receptor binding, and immunological activities.	8-anilino-1-naphthalenesulfonic acid	134-171	hypertrichosis 2 (generalised, congenital)	Homo sapiens	19-22
6250577-4	1980	The fluorescence intensity of 8-anilino-1-naphthalenesulfonate (Ans) was enhanced 27-fold with an emission maximum shift from 540 to 488 nm in the presence of CaM and Ca2+.	8-anilino-1-naphthalenesulfonic acid	30-62	calmodulin 1	Homo sapiens	159-162
6250577-4	1980	The fluorescence intensity of 8-anilino-1-naphthalenesulfonate (Ans) was enhanced 27-fold with an emission maximum shift from 540 to 488 nm in the presence of CaM and Ca2+.	8-anilino-1-naphthalenesulfonic acid	64-67	calmodulin 1	Homo sapiens	159-162
7110142-9	1982	Thus, by taking advantage of this HSOC method, whole IgG or its Fab molecules possessing very strongly hydrophobic binding sites to promote high quantum yields of 8-anilinonaphthalene-1-sulfonate (ANS) fluorescence can be easily separated.	8-anilino-1-naphthalenesulfonic acid	163-195	FA complementation group B	Homo sapiens	64-67
7110142-9	1982	Thus, by taking advantage of this HSOC method, whole IgG or its Fab molecules possessing very strongly hydrophobic binding sites to promote high quantum yields of 8-anilinonaphthalene-1-sulfonate (ANS) fluorescence can be easily separated.	8-anilino-1-naphthalenesulfonic acid	197-200	FA complementation group B	Homo sapiens	64-67
7298649-6	1981	The extent of the increase in 8-anilino-1-naphthalene sulfonate fluorescence correlates roughly with the loss of antithrombin activity and with the extent of protein aggregation as determined by high pressure liquid chromatography.	8-anilino-1-naphthalenesulfonic acid	30-63	serpin family C member 1	Homo sapiens	113-125
7225369-3	1981	The probe 8-anilinonaphthalene-1-sulfonate (ANS) was found to bind to both monomeric and dimeric IgA with comparable affinity.	8-anilino-1-naphthalenesulfonic acid	10-42	CD79a molecule	Homo sapiens	97-100
7225369-3	1981	The probe 8-anilinonaphthalene-1-sulfonate (ANS) was found to bind to both monomeric and dimeric IgA with comparable affinity.	8-anilino-1-naphthalenesulfonic acid	44-47	CD79a molecule	Homo sapiens	97-100
6250577-10	1980	The Ca2+-dependent binding of the phosphodiesterase to CaM-Sepharose was also inhibited by TFP, 9AC, and Ans.	8-anilino-1-naphthalenesulfonic acid	105-108	calmodulin 1	Homo sapiens	55-58
4734234-0	1973	Analysis of the circular dichroism of the complexes of 8-anilino-1-naphthalenesulfonate with bovine serum albumin.	8-anilino-1-naphthalenesulfonic acid	55-87	albumin	Homo sapiens	100-113
956145-1	1976	The interaction of hydrophobic probes, 8-anilinonaphthalene-1-sulfonate (ANS) and 4-benzoylamido-4"-aminostilbene-2, 2"-disulfonate (MBAS), with pig heart lipoamide dehydrogenase [NADH: lipoamide oxidoreductase, EC 1.6.4.3] was investigated.	8-anilino-1-naphthalenesulfonic acid	39-71	dihydrolipoamide dehydrogenase	Sus scrofa	155-178
956145-2	1976	When ANS or MBAS was mixed with the apoenzyme of lipoamide dehydrogenase, the fluorescence quantum yield, of each dye was enhancedd markedly and the emission maxima concurrently shifted to the blue.	8-anilino-1-naphthalenesulfonic acid	5-8	dihydrolipoamide dehydrogenase	Sus scrofa	49-72
956145-6	1976	These results indicate that ANS or MBAS bound to the apoenzyme of lipoamide dehydrogenase is situated in a hydrophobic region of the apoenzyme molecule.	8-anilino-1-naphthalenesulfonic acid	28-31	dihydrolipoamide dehydrogenase	Sus scrofa	66-89
1033907-2	1976	The fluorescence probe ANS(8-anilino-1-naphthalenesulfonic acid) was employed as a reporter group molecule for circular dichroism and fluorescence measurements in order to investigate the effects of stearic acid and sodium dodecylsulfate on the conformation of bovine and human serum albumin.	8-anilino-1-naphthalenesulfonic acid	27-63	albumin	Bos taurus	278-291
1182114-2	1975	Human chorionic gonadotropin (hCG) self-associates to form higher molecular weight species in the presence of the fluorescence probe 8-anilino-1-naphthalenesulfonate (ANS).	8-anilino-1-naphthalenesulfonic acid	133-165	chorionic gonadotropin subunit beta 5	Homo sapiens	6-34
1182114-2	1975	Human chorionic gonadotropin (hCG) self-associates to form higher molecular weight species in the presence of the fluorescence probe 8-anilino-1-naphthalenesulfonate (ANS).	8-anilino-1-naphthalenesulfonic acid	167-170	chorionic gonadotropin subunit beta 5	Homo sapiens	6-34
27060481-0	2016	Thermodynamic analysis of ANS binding to partially unfolded alpha-lactalbumin: correlation of endothermic to exothermic changeover with formation of authentic molten globules.	8-anilino-1-naphthalenesulfonic acid	26-29	lactalbumin alpha	Bos taurus	60-77
33466888-4	2021	In this study, to reveal the rate-limiting step of the ANS-protein binding process, protein concentration-dependent measurements of the ANS fluorescence of lysozyme and bovine serum albumin were performed, and the binding constants were analyzed.	8-anilino-1-naphthalenesulfonic acid	55-58	lysozyme	Homo sapiens	156-164
33466888-4	2021	In this study, to reveal the rate-limiting step of the ANS-protein binding process, protein concentration-dependent measurements of the ANS fluorescence of lysozyme and bovine serum albumin were performed, and the binding constants were analyzed.	8-anilino-1-naphthalenesulfonic acid	55-58	albumin	Homo sapiens	176-189
33466888-4	2021	In this study, to reveal the rate-limiting step of the ANS-protein binding process, protein concentration-dependent measurements of the ANS fluorescence of lysozyme and bovine serum albumin were performed, and the binding constants were analyzed.	8-anilino-1-naphthalenesulfonic acid	136-139	lysozyme	Homo sapiens	156-164
32433863-3	2020	Here, we detail the positive cooperativity between ANS and orthosteric Cdk2 inhibitors dinaciclib and roscovitine, which increase the affinity of ANS toward Cdk2 5-fold to 10-fold, and the relatively noncooperative effects of ATP.	8-anilino-1-naphthalenesulfonic acid	146-149	cyclin dependent kinase 2	Homo sapiens	71-75
32433863-3	2020	Here, we detail the positive cooperativity between ANS and orthosteric Cdk2 inhibitors dinaciclib and roscovitine, which increase the affinity of ANS toward Cdk2 5-fold to 10-fold, and the relatively noncooperative effects of ATP.	8-anilino-1-naphthalenesulfonic acid	146-149	cyclin dependent kinase 2	Homo sapiens	157-161
31612448-4	2020	Here we describe the characterization beta-casein nanocarriers encapsulating a model hydrophobic compound, 8-anilino-1-naphthalenesulfonic acid, and the natural bioactive curcumin using the Malvern NanoSight NS300.	8-anilino-1-naphthalenesulfonic acid	107-143	casein beta	Homo sapiens	38-49
28025170-9	2017	The C154F and C154Y variants induced secondary and tertiary structural changes in AKR1C3 at 40  C as reflected by their altered circular dichroism and 8-anilinonaphthalene-1-sulfonate fluorescence characteristics.	8-anilino-1-naphthalenesulfonic acid	151-183	aldo-keto reductase family 1 member C3	Homo sapiens	82-88
33935286-5	2022	We demonstrated that synthetic FABP7 ligand 6 displayed a high affinity against FABP7 with Kd value of 209 nM assessed in 8-anilinonaphthalene-1-sulfonic acid (ANS) assay; ligand 6 improved U251 cell survival via disrupting the FABP7-Syn interaction.	8-anilino-1-naphthalenesulfonic acid	122-158	fatty acid binding protein 7	Homo sapiens	31-36
33935286-5	2022	We demonstrated that synthetic FABP7 ligand 6 displayed a high affinity against FABP7 with Kd value of 209 nM assessed in 8-anilinonaphthalene-1-sulfonic acid (ANS) assay; ligand 6 improved U251 cell survival via disrupting the FABP7-Syn interaction.	8-anilino-1-naphthalenesulfonic acid	122-158	fatty acid binding protein 7	Homo sapiens	80-85
33935286-5	2022	We demonstrated that synthetic FABP7 ligand 6 displayed a high affinity against FABP7 with Kd value of 209 nM assessed in 8-anilinonaphthalene-1-sulfonic acid (ANS) assay; ligand 6 improved U251 cell survival via disrupting the FABP7-Syn interaction.	8-anilino-1-naphthalenesulfonic acid	122-158	fatty acid binding protein 7	Homo sapiens	80-85
33935286-5	2022	We demonstrated that synthetic FABP7 ligand 6 displayed a high affinity against FABP7 with Kd value of 209 nM assessed in 8-anilinonaphthalene-1-sulfonic acid (ANS) assay; ligand 6 improved U251 cell survival via disrupting the FABP7-Syn interaction.	8-anilino-1-naphthalenesulfonic acid	160-163	fatty acid binding protein 7	Homo sapiens	31-36
33935286-5	2022	We demonstrated that synthetic FABP7 ligand 6 displayed a high affinity against FABP7 with Kd value of 209 nM assessed in 8-anilinonaphthalene-1-sulfonic acid (ANS) assay; ligand 6 improved U251 cell survival via disrupting the FABP7-Syn interaction.	8-anilino-1-naphthalenesulfonic acid	160-163	fatty acid binding protein 7	Homo sapiens	80-85
33935286-5	2022	We demonstrated that synthetic FABP7 ligand 6 displayed a high affinity against FABP7 with Kd value of 209 nM assessed in 8-anilinonaphthalene-1-sulfonic acid (ANS) assay; ligand 6 improved U251 cell survival via disrupting the FABP7-Syn interaction.	8-anilino-1-naphthalenesulfonic acid	160-163	fatty acid binding protein 7	Homo sapiens	80-85
32433863-0	2020	Cooperativity Between Orthosteric Inhibitors and Allosteric Inhibitor 8-Anilino-1-Naphthalene Sulfonic Acid (ANS) in Cyclin-Dependent Kinase 2.	8-anilino-1-naphthalenesulfonic acid	70-107	cyclin dependent kinase 2	Homo sapiens	117-142
32433863-0	2020	Cooperativity Between Orthosteric Inhibitors and Allosteric Inhibitor 8-Anilino-1-Naphthalene Sulfonic Acid (ANS) in Cyclin-Dependent Kinase 2.	8-anilino-1-naphthalenesulfonic acid	109-112	cyclin dependent kinase 2	Homo sapiens	117-142
32433863-2	2020	We have previously discovered that 8-anilino-1-naphthalene sulfonic acid (ANS) binds an allosteric pocket in cyclin-dependent kinase 2 (Cdk2).	8-anilino-1-naphthalenesulfonic acid	35-72	cyclin dependent kinase 2	Homo sapiens	109-134
32433863-2	2020	We have previously discovered that 8-anilino-1-naphthalene sulfonic acid (ANS) binds an allosteric pocket in cyclin-dependent kinase 2 (Cdk2).	8-anilino-1-naphthalenesulfonic acid	35-72	cyclin dependent kinase 2	Homo sapiens	136-140
32433863-2	2020	We have previously discovered that 8-anilino-1-naphthalene sulfonic acid (ANS) binds an allosteric pocket in cyclin-dependent kinase 2 (Cdk2).	8-anilino-1-naphthalenesulfonic acid	74-77	cyclin dependent kinase 2	Homo sapiens	109-134
32433863-2	2020	We have previously discovered that 8-anilino-1-naphthalene sulfonic acid (ANS) binds an allosteric pocket in cyclin-dependent kinase 2 (Cdk2).	8-anilino-1-naphthalenesulfonic acid	74-77	cyclin dependent kinase 2	Homo sapiens	136-140
32433863-3	2020	Here, we detail the positive cooperativity between ANS and orthosteric Cdk2 inhibitors dinaciclib and roscovitine, which increase the affinity of ANS toward Cdk2 5-fold to 10-fold, and the relatively noncooperative effects of ATP.	8-anilino-1-naphthalenesulfonic acid	51-54	cyclin dependent kinase 2	Homo sapiens	157-161
30496735-4	2019	We developed FABP3 ligands, which bind to the fatty acid binding domain of FABP3, using an 8-Anilinonaphthalene-1-sulfonic acid (ANS) assay with a recombinant FABP3 protein.	8-anilino-1-naphthalenesulfonic acid	91-127	fatty acid binding protein 3, muscle and heart	Mus musculus	13-18
30496735-4	2019	We developed FABP3 ligands, which bind to the fatty acid binding domain of FABP3, using an 8-Anilinonaphthalene-1-sulfonic acid (ANS) assay with a recombinant FABP3 protein.	8-anilino-1-naphthalenesulfonic acid	91-127	fatty acid binding protein 3, muscle and heart	Mus musculus	75-80
30496735-4	2019	We developed FABP3 ligands, which bind to the fatty acid binding domain of FABP3, using an 8-Anilinonaphthalene-1-sulfonic acid (ANS) assay with a recombinant FABP3 protein.	8-anilino-1-naphthalenesulfonic acid	91-127	fatty acid binding protein 3, muscle and heart	Mus musculus	75-80
30496735-4	2019	We developed FABP3 ligands, which bind to the fatty acid binding domain of FABP3, using an 8-Anilinonaphthalene-1-sulfonic acid (ANS) assay with a recombinant FABP3 protein.	8-anilino-1-naphthalenesulfonic acid	129-132	fatty acid binding protein 3, muscle and heart	Mus musculus	13-18
30496735-4	2019	We developed FABP3 ligands, which bind to the fatty acid binding domain of FABP3, using an 8-Anilinonaphthalene-1-sulfonic acid (ANS) assay with a recombinant FABP3 protein.	8-anilino-1-naphthalenesulfonic acid	129-132	fatty acid binding protein 3, muscle and heart	Mus musculus	75-80
30496735-4	2019	We developed FABP3 ligands, which bind to the fatty acid binding domain of FABP3, using an 8-Anilinonaphthalene-1-sulfonic acid (ANS) assay with a recombinant FABP3 protein.	8-anilino-1-naphthalenesulfonic acid	129-132	fatty acid binding protein 3, muscle and heart	Mus musculus	75-80
30023748-4	2017	In lysozyme, the location of the drug was ascertained by competitive binding assay with 8-anilino-1-naphthalenesulfonate, and this was further examined with molecular docking simulation.	8-anilino-1-naphthalenesulfonic acid	88-120	lysozyme	Homo sapiens	3-11
27191938-4	2016	The interaction of HFB1 with hydrophobic sites on the surface of luciferase was confirmed by extrinsic fluorescence studies using 8-anilino-1-naphthalenesulfonic acid (ANS) as a hydrophobic reporter probe.	8-anilino-1-naphthalenesulfonic acid	130-166	complexin 2	Homo sapiens	19-23
27385129-2	2016	ANS complexation by cyclodextrins or bovine serum albumin (BSA) results in a nonhomogeneous solvation shell that is reflected by nonsynchronous variation of fluorescence properties.	8-anilino-1-naphthalenesulfonic acid	0-3	albumin	Homo sapiens	44-57
27191938-4	2016	The interaction of HFB1 with hydrophobic sites on the surface of luciferase was confirmed by extrinsic fluorescence studies using 8-anilino-1-naphthalenesulfonic acid (ANS) as a hydrophobic reporter probe.	8-anilino-1-naphthalenesulfonic acid	168-171	complexin 2	Homo sapiens	19-23
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	11-44	cyclin dependent kinase 2	Homo sapiens	51-55
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	11-44	cyclin dependent kinase 2	Homo sapiens	94-98
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	46-49	cyclin dependent kinase 2	Homo sapiens	51-55
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	46-49	cyclin dependent kinase 2	Homo sapiens	94-98
26685215-8	2016	Second, in 8-anilino-1-naphthalene-sulfonate (ANS)-CDK2 fluorescence assays, preincubation of CDK2 with salicylic acid dose-dependently quenched the fluorescence due to ANS.	8-anilino-1-naphthalenesulfonic acid	169-172	cyclin dependent kinase 2	Homo sapiens	94-98
26349210-8	2015	The equilibrium unfolding mechanism of dihydrofolate reductase proteins using guanidine hydrochloride as a denaturant in the presence of various types of osmolytes has been monitored using loss in enzymatic activity, intrinsic tryptophan fluorescence and an extrinsic fluorophore 8-anilino-1-naphthalene-sulfonic acid as probes.	8-anilino-1-naphthalenesulfonic acid	280-317	dihydrofolate reductase	Homo sapiens	39-62
25837412-4	2015	With the aid of hydrophobic ANS experiments, subdomain IIA and alpha1beta2 interface of albumin and hemoglobin, respectively, were found to be preserved high-affinity for imidacloprid.	8-anilino-1-naphthalenesulfonic acid	28-31	albumin	Homo sapiens	88-95
23090025-2	2013	Conformational changes on ovalbumin at various concentrations of glyoxal, ethylene glycol (EG) and polyethylene glycol-400 (PEG-400) were investigated by fluorescence spectroscopy, circular dichroism, attenuated total reflection Fourier transform infra red spectroscopy, 8-anilino-1-naphthalenesulfonic acid and thioflavin T assay.	8-anilino-1-naphthalenesulfonic acid	271-307	ovalbumin (SERPINB14)	Gallus gallus	26-35
25457456-6	2015	FABP4 binding affinities for chemically synthesized FTAP1 and FTAP3 were measured using FABP4 and 8-anilino-1-naphthalene sulfonic acid.	8-anilino-1-naphthalenesulfonic acid	98-135	fatty acid binding protein 4, adipocyte	Mus musculus	0-5
25314129-6	2014	The binding of phenethyl ferulate and the selected compounds to TTR were confirmed by the 8-anilino-1-naphthalenesulfonic acid displacement and X-ray crystallography.	8-anilino-1-naphthalenesulfonic acid	90-126	transthyretin	Homo sapiens	64-67
24531481-6	2014	The resulting structure of Hyp-1, a pathogenesis-related class 10 (PR-10) protein from the medicinal herb St John"s wort, reveals the binding modes of the fluorescent probe 8-anilino-1-naphthalene sulfonate (ANS), providing insight into the function of the protein in binding or storing hydrophobic ligands.	8-anilino-1-naphthalenesulfonic acid	173-206	phosphate regulating endopeptidase homolog X-linked	Homo sapiens	27-32
24531481-6	2014	The resulting structure of Hyp-1, a pathogenesis-related class 10 (PR-10) protein from the medicinal herb St John"s wort, reveals the binding modes of the fluorescent probe 8-anilino-1-naphthalene sulfonate (ANS), providing insight into the function of the protein in binding or storing hydrophobic ligands.	8-anilino-1-naphthalenesulfonic acid	208-211	phosphate regulating endopeptidase homolog X-linked	Homo sapiens	27-32
23397429-8	2013	Structural changes of microtubule and tau protein, as an essential microtubule-associated protein for tubulin assembly, were detected via circular dichroism spectroscopy, intrinsic fluorescence, and 8-anilino-1-naphthalenesulfonic acid fluorometry.	8-anilino-1-naphthalenesulfonic acid	199-235	microtubule associated protein tau	Homo sapiens	38-41
25224297-7	2015	The relative surface hydrophobicity of standard and released insulin from the SMGel was estimated using 8-anilino-1-naphthalene sulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	143-146	insulin	Homo sapiens	61-68
24729012-4	2014	Cyt c exists as molten globule (MG) at 60% PEG-400 and 40% TFE as confirmed by far-UV CD, attenuated total reflection Fourier transform infrared spectroscopy, Trp environment, 8-anilino-1-naphthalene-sulfonic acid (ANS) binding and blue shift in the soret band.	8-anilino-1-naphthalenesulfonic acid	176-213	cytochrome c, somatic	Homo sapiens	0-5
24729012-4	2014	Cyt c exists as molten globule (MG) at 60% PEG-400 and 40% TFE as confirmed by far-UV CD, attenuated total reflection Fourier transform infrared spectroscopy, Trp environment, 8-anilino-1-naphthalene-sulfonic acid (ANS) binding and blue shift in the soret band.	8-anilino-1-naphthalenesulfonic acid	215-218	cytochrome c, somatic	Homo sapiens	0-5
24732569-7	2014	The affinity of synthesized TAP1 was measured using FABP4 and 8-anilino-1-naphthalene sulfonic acid.	8-anilino-1-naphthalenesulfonic acid	62-99	transporter 1, ATP-binding cassette, sub-family B (MDR/TAP)	Mus musculus	28-32
18652480-8	2008	In a manner similar to that of known helix bundle apolipoproteins, apoA-V(1-146) induced a relatively small enhancement in 8-anilino-1-naphthalenesulfonic acid fluorescence intensity.	8-anilino-1-naphthalenesulfonic acid	123-159	apolipoprotein A5	Homo sapiens	67-73
22893598-2	2012	The assay is based on the specific interaction of CDK2 with the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS), which binds to a large allosteric pocket adjacent to the ATP site.	8-anilino-1-naphthalenesulfonic acid	86-119	cyclin dependent kinase 2	Homo sapiens	50-54
22893598-2	2012	The assay is based on the specific interaction of CDK2 with the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS), which binds to a large allosteric pocket adjacent to the ATP site.	8-anilino-1-naphthalenesulfonic acid	121-124	cyclin dependent kinase 2	Homo sapiens	50-54
22893598-3	2012	Hit compounds that displace ANS directly or indirectly from CDK2 are readily classified as ATP site binders or allosteric ligands through the use of staurosporine, which blocks the ATP site without displacing ANS.	8-anilino-1-naphthalenesulfonic acid	28-31	cyclin dependent kinase 2	Homo sapiens	60-64
22893598-3	2012	Hit compounds that displace ANS directly or indirectly from CDK2 are readily classified as ATP site binders or allosteric ligands through the use of staurosporine, which blocks the ATP site without displacing ANS.	8-anilino-1-naphthalenesulfonic acid	209-212	cyclin dependent kinase 2	Homo sapiens	60-64
22391886-6	2012	Stability of released insulin was investigated by 8-anilino-1-naphthalenesulfonate probe.	8-anilino-1-naphthalenesulfonic acid	50-82	insulin	Homo sapiens	22-29
22306744-10	2012	And the results, showed by ANS binding and fluorescence quenching, indicated that a pH-dependent conformational change of Tim23(IMS) occurs, and at pH 4 and 3, it forms a compact structure.	8-anilino-1-naphthalenesulfonic acid	27-30	translocase of inner mitochondrial membrane 23	Homo sapiens	122-132
21291269-5	2011	In an effort to identify potential target sites for disruption of the CDK-cyclin interaction, we probed the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS) with human CDK2 and cyclin A using fluorescence spectroscopy and protein crystallography.	8-anilino-1-naphthalenesulfonic acid	130-163	proliferating cell nuclear antigen	Homo sapiens	74-80
21291269-5	2011	In an effort to identify potential target sites for disruption of the CDK-cyclin interaction, we probed the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS) with human CDK2 and cyclin A using fluorescence spectroscopy and protein crystallography.	8-anilino-1-naphthalenesulfonic acid	130-163	cyclin dependent kinase 2	Homo sapiens	181-185
21291269-5	2011	In an effort to identify potential target sites for disruption of the CDK-cyclin interaction, we probed the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS) with human CDK2 and cyclin A using fluorescence spectroscopy and protein crystallography.	8-anilino-1-naphthalenesulfonic acid	130-163	cyclin A2	Homo sapiens	190-198
21291269-5	2011	In an effort to identify potential target sites for disruption of the CDK-cyclin interaction, we probed the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS) with human CDK2 and cyclin A using fluorescence spectroscopy and protein crystallography.	8-anilino-1-naphthalenesulfonic acid	165-168	proliferating cell nuclear antigen	Homo sapiens	74-80
21291269-5	2011	In an effort to identify potential target sites for disruption of the CDK-cyclin interaction, we probed the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS) with human CDK2 and cyclin A using fluorescence spectroscopy and protein crystallography.	8-anilino-1-naphthalenesulfonic acid	165-168	cyclin dependent kinase 2	Homo sapiens	181-185
21291269-5	2011	In an effort to identify potential target sites for disruption of the CDK-cyclin interaction, we probed the extrinsic fluorophore 8-anilino-1-naphthalene sulfonate (ANS) with human CDK2 and cyclin A using fluorescence spectroscopy and protein crystallography.	8-anilino-1-naphthalenesulfonic acid	165-168	cyclin A2	Homo sapiens	190-198
21291269-6	2011	ANS interacts with free CDK2 in a saturation-dependent manner with an apparent K(d) of 37 muM, and cyclin A displaced ANS from CDK2 with an EC(50) value of 0.6 muM.	8-anilino-1-naphthalenesulfonic acid	0-3	cyclin dependent kinase 2	Homo sapiens	24-28
21291269-6	2011	ANS interacts with free CDK2 in a saturation-dependent manner with an apparent K(d) of 37 muM, and cyclin A displaced ANS from CDK2 with an EC(50) value of 0.6 muM.	8-anilino-1-naphthalenesulfonic acid	118-121	cyclin A2	Homo sapiens	99-107
21291269-6	2011	ANS interacts with free CDK2 in a saturation-dependent manner with an apparent K(d) of 37 muM, and cyclin A displaced ANS from CDK2 with an EC(50) value of 0.6 muM.	8-anilino-1-naphthalenesulfonic acid	118-121	cyclin dependent kinase 2	Homo sapiens	127-131
21291269-8	2011	Binding of ANS is accompanied by substantial structural changes in CDK2, resulting in a C-helix conformation that is incompatible for cyclin A association.	8-anilino-1-naphthalenesulfonic acid	11-14	cyclin dependent kinase 2	Homo sapiens	67-71
21291269-8	2011	Binding of ANS is accompanied by substantial structural changes in CDK2, resulting in a C-helix conformation that is incompatible for cyclin A association.	8-anilino-1-naphthalenesulfonic acid	11-14	cyclin A2	Homo sapiens	134-142
21291269-9	2011	These findings indicate the potential of the ANS binding pocket as a new target site for allosteric inhibitors disrupting the interaction of CDKs and cyclins.	8-anilino-1-naphthalenesulfonic acid	45-48	cyclin dependent kinase 2	Homo sapiens	141-145
21517007-7	2011	Similarly, by employing 8-anilino-1-naphthalenesulfonic acid as fluorescence probe, the binding affinity of BPA with TTR and TBG was measured to be 3.10 x 10(5) and 5.90 x 10(5) L/mol, respectively.	8-anilino-1-naphthalenesulfonic acid	24-60	transthyretin	Homo sapiens	117-120
21517007-7	2011	Similarly, by employing 8-anilino-1-naphthalenesulfonic acid as fluorescence probe, the binding affinity of BPA with TTR and TBG was measured to be 3.10 x 10(5) and 5.90 x 10(5) L/mol, respectively.	8-anilino-1-naphthalenesulfonic acid	24-60	serpin family A member 7	Homo sapiens	125-128
20466559-1	2010	The effect of reversible removal of HCO(3)(-) on structural re-arrangements in the Mn-stabilizing protein (MSP) of photosystem II, isolated from pea leaves, was studied using measurements of characteristic alterations in fluorescence of hydrophobic probe 8-anilino-1-naphthalene-sulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	255-292	microseminoprotein beta	Homo sapiens	107-110
20466559-1	2010	The effect of reversible removal of HCO(3)(-) on structural re-arrangements in the Mn-stabilizing protein (MSP) of photosystem II, isolated from pea leaves, was studied using measurements of characteristic alterations in fluorescence of hydrophobic probe 8-anilino-1-naphthalene-sulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	294-297	microseminoprotein beta	Homo sapiens	107-110
19594832-7	2009	Equine lysozyme acquired a partially unfolded conformation in ELOA, as evident from its ability to bind hydrophobic dye 8-anilinonaphthalene-1-sulfonate.	8-anilino-1-naphthalenesulfonic acid	120-152	elongin A	Homo sapiens	62-66
19016256-7	2008	However, comparison of MS and fluorescence data at variable pH for BLG and myoglobin (Mb) suggests that conformation-specific ANS binding to proteins is detectable by ESI-MS only inside well-structured cavities of folded structures, like the BLG calyx and apoMb heme pocket.	8-anilino-1-naphthalenesulfonic acid	126-129	myoglobin	Homo sapiens	75-84
18645209-4	2008	Circular dichroism spectral measurements and 8-anilino-1-naphthalenesulfonic acid fluorescence measurements of lipid-free apoA-I ascribed this accelerated rHDL formation to the conformational change of the protein into a rather hydrophobic alpha-helical structure under acidic conditions.	8-anilino-1-naphthalenesulfonic acid	45-81	apolipoprotein A1	Homo sapiens	122-128
18485727-0	2008	Binding of 8-anilino-1-naphthalenesulfonate to lecithin:cholesterol acyltransferase studied by fluorescence techniques.	8-anilino-1-naphthalenesulfonic acid	11-43	lecithin-cholesterol acyltransferase	Homo sapiens	47-83
18485727-5	2008	In the later case, not only ANS but also tryptophan (Trp) residues of LCAT is being excited.	8-anilino-1-naphthalenesulfonic acid	28-31	lecithin-cholesterol acyltransferase	Homo sapiens	70-74
18485727-9	2008	The lifetime of ANS bound to LCAT was above 16 ns which is characteristic for it being in a hydrophobic environment.	8-anilino-1-naphthalenesulfonic acid	16-19	lecithin-cholesterol acyltransferase	Homo sapiens	29-33
18485727-10	2008	The ANS labeled LCAT fluorescence anisotropy decay revealed the correlation time of 42 ns with a weak residual motion of 2.8 ns.	8-anilino-1-naphthalenesulfonic acid	4-7	lecithin-cholesterol acyltransferase	Homo sapiens	16-20
18485727-1	2008	The solvatochromic fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS) has been used to study the hydrophobicity and conformational dynamics of lecithin:cholesterol acyltransferase (LCAT).	8-anilino-1-naphthalenesulfonic acid	37-69	lecithin-cholesterol acyltransferase	Homo sapiens	149-185
18485727-11	2008	These characteristics of ANS labeled LCAT fluorescence show that ANS is an excellent probe to study conformational changes of LCAT protein and its interactions with other macromolecules.	8-anilino-1-naphthalenesulfonic acid	25-28	lecithin-cholesterol acyltransferase	Homo sapiens	37-41
18485727-11	2008	These characteristics of ANS labeled LCAT fluorescence show that ANS is an excellent probe to study conformational changes of LCAT protein and its interactions with other macromolecules.	8-anilino-1-naphthalenesulfonic acid	25-28	lecithin-cholesterol acyltransferase	Homo sapiens	126-130
18485727-11	2008	These characteristics of ANS labeled LCAT fluorescence show that ANS is an excellent probe to study conformational changes of LCAT protein and its interactions with other macromolecules.	8-anilino-1-naphthalenesulfonic acid	65-68	lecithin-cholesterol acyltransferase	Homo sapiens	37-41
18485727-11	2008	These characteristics of ANS labeled LCAT fluorescence show that ANS is an excellent probe to study conformational changes of LCAT protein and its interactions with other macromolecules.	8-anilino-1-naphthalenesulfonic acid	65-68	lecithin-cholesterol acyltransferase	Homo sapiens	126-130
18485727-1	2008	The solvatochromic fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS) has been used to study the hydrophobicity and conformational dynamics of lecithin:cholesterol acyltransferase (LCAT).	8-anilino-1-naphthalenesulfonic acid	37-69	lecithin-cholesterol acyltransferase	Homo sapiens	187-191
18485727-1	2008	The solvatochromic fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS) has been used to study the hydrophobicity and conformational dynamics of lecithin:cholesterol acyltransferase (LCAT).	8-anilino-1-naphthalenesulfonic acid	71-74	lecithin-cholesterol acyltransferase	Homo sapiens	149-185
18485727-1	2008	The solvatochromic fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS) has been used to study the hydrophobicity and conformational dynamics of lecithin:cholesterol acyltransferase (LCAT).	8-anilino-1-naphthalenesulfonic acid	71-74	lecithin-cholesterol acyltransferase	Homo sapiens	187-191
18485727-2	2008	The ANS to LCAT binding constant was estimated from titrations with ANS, keeping a constant concentration of LCAT (2 microM).	8-anilino-1-naphthalenesulfonic acid	4-7	lecithin-cholesterol acyltransferase	Homo sapiens	11-15
18485727-2	2008	The ANS to LCAT binding constant was estimated from titrations with ANS, keeping a constant concentration of LCAT (2 microM).	8-anilino-1-naphthalenesulfonic acid	4-7	lecithin-cholesterol acyltransferase	Homo sapiens	109-113
18485727-2	2008	The ANS to LCAT binding constant was estimated from titrations with ANS, keeping a constant concentration of LCAT (2 microM).	8-anilino-1-naphthalenesulfonic acid	68-71	lecithin-cholesterol acyltransferase	Homo sapiens	11-15
18336901-5	2008	Fluorescence studies of 8-anilino-1-naphthalenesulfonic acid (ANS) binding demonstrated that bovine beta-CN is doted with larger effective hydrophobic surfaces at all studied temperatures than camel beta-CN.	8-anilino-1-naphthalenesulfonic acid	24-60	casein beta	Bos taurus	100-107
18336901-5	2008	Fluorescence studies of 8-anilino-1-naphthalenesulfonic acid (ANS) binding demonstrated that bovine beta-CN is doted with larger effective hydrophobic surfaces at all studied temperatures than camel beta-CN.	8-anilino-1-naphthalenesulfonic acid	62-65	casein beta	Bos taurus	100-107
16878982-11	2006	Using stopped-flow fluorescence spectroscopy in the presence of 8-anilino-1-naphthalene sulfonic acid (ANS), we show that RBP populates a state with molten-globule-like characteristics early in refolding.	8-anilino-1-naphthalenesulfonic acid	64-101	retinol binding protein 4	Homo sapiens	122-125
16741984-0	2006	Elucidating the binding thermodynamics of 8-anilino-1-naphthalene sulfonic acid with the A-state of alpha-lactalbumin: an isothermal titration calorimetric investigation.	8-anilino-1-naphthalenesulfonic acid	42-79	lactalbumin alpha	Homo sapiens	100-117
16741984-1	2006	Isothermal titration calorimetry has been used to demonstrate that the heat profile associated with the binding of 8-anilino-1-naphthalene sulfonic acid (ANS) with the acid induced molten globule state (A-state) of alpha-lactalbumin (alpha-LA) is different from that with the native and denatured states of the protein.	8-anilino-1-naphthalenesulfonic acid	115-152	lactalbumin alpha	Homo sapiens	215-232
16741984-1	2006	Isothermal titration calorimetry has been used to demonstrate that the heat profile associated with the binding of 8-anilino-1-naphthalene sulfonic acid (ANS) with the acid induced molten globule state (A-state) of alpha-lactalbumin (alpha-LA) is different from that with the native and denatured states of the protein.	8-anilino-1-naphthalenesulfonic acid	115-152	lactalbumin alpha	Homo sapiens	234-242
16741984-1	2006	Isothermal titration calorimetry has been used to demonstrate that the heat profile associated with the binding of 8-anilino-1-naphthalene sulfonic acid (ANS) with the acid induced molten globule state (A-state) of alpha-lactalbumin (alpha-LA) is different from that with the native and denatured states of the protein.	8-anilino-1-naphthalenesulfonic acid	154-157	lactalbumin alpha	Homo sapiens	215-232
16741984-1	2006	Isothermal titration calorimetry has been used to demonstrate that the heat profile associated with the binding of 8-anilino-1-naphthalene sulfonic acid (ANS) with the acid induced molten globule state (A-state) of alpha-lactalbumin (alpha-LA) is different from that with the native and denatured states of the protein.	8-anilino-1-naphthalenesulfonic acid	154-157	lactalbumin alpha	Homo sapiens	234-242
16741984-3	2006	ANS binds to the A-state of alpha-LA at two independent binding sites that remain nearly the same in the temperature range of 10-35 degrees C. The number of moles of ANS binding at site 1 at 10 degrees C is 14.0+/-0.2 and remains nearly the same with rise in temperature.	8-anilino-1-naphthalenesulfonic acid	0-3	lactalbumin alpha	Homo sapiens	28-36
16741984-3	2006	ANS binds to the A-state of alpha-LA at two independent binding sites that remain nearly the same in the temperature range of 10-35 degrees C. The number of moles of ANS binding at site 1 at 10 degrees C is 14.0+/-0.2 and remains nearly the same with rise in temperature.	8-anilino-1-naphthalenesulfonic acid	166-169	lactalbumin alpha	Homo sapiens	28-36
17907278-6	2007	To obtain further insight into the nature of the interaction between cyt c and the bilayers, the competition between 8-anilino-1-naphthalene sulfonate (ANS) and the protein for the same binding sites in liposomes was monitored by fluorescence.	8-anilino-1-naphthalenesulfonic acid	117-150	cytochrome c, somatic	Homo sapiens	69-74
16878982-11	2006	Using stopped-flow fluorescence spectroscopy in the presence of 8-anilino-1-naphthalene sulfonic acid (ANS), we show that RBP populates a state with molten-globule-like characteristics early in refolding.	8-anilino-1-naphthalenesulfonic acid	103-106	retinol binding protein 4	Homo sapiens	122-125
16951992-2	2006	8-Anilinonaphthalene- 1-sulfonate fluorescence measurement showed that KChIP4.1 and KChIP2.2 possessed one and two types of Ca2+-binding sites, respectively, and only one type of Mg2+-binding site was noted in the two KChIP proteins.	8-anilino-1-naphthalenesulfonic acid	0-33	potassium voltage-gated channel interacting protein 2	Homo sapiens	84-90
16877768-3	2006	At 30% (v/v) methanol, rmethuG-CSF has ANS binding ability.	8-anilino-1-naphthalenesulfonic acid	39-42	colony stimulating factor 2	Homo sapiens	31-34
15757902-5	2005	Disruption of the N-capping motif of helix 9 in hGST A1-1 alters the conformational dynamics of the C-terminal region and, consequently, the features of the H-site to which hydrophobic substrates (e.g. 1-chloro-2,4-dinitrobenzene (CDNB)) and nonsubstrates (e.g. 8-anilino-1-naphthalene sulfonate (ANS)) bind.	8-anilino-1-naphthalenesulfonic acid	262-295	glutathione S-transferase alpha 1	Homo sapiens	48-57
16548517-0	2006	Temperature dependence of benzyl alcohol- and 8-anilinonaphthalene-1-sulfonate-induced aggregation of recombinant human interleukin-1 receptor antagonist.	8-anilino-1-naphthalenesulfonic acid	46-78	interleukin 1 receptor antagonist	Homo sapiens	120-153
16190664-0	2005	Influence of binding of sodium dodecyl sulfate, all-trans-retinol, and 8-anilino-1-naphthalenesulfonate on the high-pressure-induced unfolding and aggregation of beta-lactoglobulin B.	8-anilino-1-naphthalenesulfonic acid	71-103	beta-lactoglobulin	Bos taurus	162-182
16599727-0	2006	Temperature dependence of solvation dynamics and anisotropy decay in a protein: ANS in bovine serum albumin.	8-anilino-1-naphthalenesulfonic acid	80-83	albumin	Homo sapiens	94-107
16599727-1	2006	Temperature dependence of solvation dynamics and fluorescence anisotropy decay of 8-anilino-1-naphthalenesulfonate (ANS) bound to a protein, bovine serum albumin (BSA), are studied.	8-anilino-1-naphthalenesulfonic acid	82-114	albumin	Homo sapiens	148-161
16599727-1	2006	Temperature dependence of solvation dynamics and fluorescence anisotropy decay of 8-anilino-1-naphthalenesulfonate (ANS) bound to a protein, bovine serum albumin (BSA), are studied.	8-anilino-1-naphthalenesulfonic acid	116-119	albumin	Homo sapiens	148-161
15757902-5	2005	Disruption of the N-capping motif of helix 9 in hGST A1-1 alters the conformational dynamics of the C-terminal region and, consequently, the features of the H-site to which hydrophobic substrates (e.g. 1-chloro-2,4-dinitrobenzene (CDNB)) and nonsubstrates (e.g. 8-anilino-1-naphthalene sulfonate (ANS)) bind.	8-anilino-1-naphthalenesulfonic acid	297-300	glutathione S-transferase alpha 1	Homo sapiens	48-57
14645081-6	2003	The presence of an equilibrium intermediate form was shown by the interaction of beta-lactoglobulin with 8-anilino-1-naphthalene sulfonic acid, a probe widely used to detect molten-globule states of proteins.	8-anilino-1-naphthalenesulfonic acid	105-142	beta-lactoglobulin	Bos taurus	81-99
15248769-2	2004	The folding process of the acylphosphatase from Sulfolobus solfataricus (Sso AcP) has been followed, starting from the fully unfolded state, using a variety of spectroscopic probes, including intrinsic fluorescence, circular dichroism, and ANS binding.	8-anilino-1-naphthalenesulfonic acid	240-243	acylphosphatase	Saccharolobus solfataricus	27-42
15102861-11	2004	8-Anilino-1-naphthalene sulfonic acid binding data showed solvation of the myristoyl group in the presence of Ca(2+), which could be attributed to the myristoyl-dependent conformational changes in NCS-1.	8-anilino-1-naphthalenesulfonic acid	0-37	neuronal calcium sensor 1	Homo sapiens	197-202
15826078-0	2005	Influence of binding of sodium dodecyl sulfate, all-trans-retinol, palmitate, and 8-anilino-1-naphthalenesulfonate on the heat-induced unfolding and aggregation of beta-lactoglobulin B.	8-anilino-1-naphthalenesulfonic acid	82-114	beta-lactoglobulin	Bos taurus	164-184
15826078-6	2005	It was also noted that holding a beta-LG solution with added SDS or ANS promoted the formation of a hydrophobically associated non-native dimer.	8-anilino-1-naphthalenesulfonic acid	68-71	beta-lactoglobulin	Bos taurus	33-40
10521270-0	1999	8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions.	8-anilino-1-naphthalenesulfonic acid	0-37	cytochrome c, somatic	Equus caballus	77-89
14592407-7	2003	However, the binding of ANS with KChIP1 is no longer observed after removal of EF-hands 3 and 4.	8-anilino-1-naphthalenesulfonic acid	24-27	potassium voltage-gated channel interacting protein 1	Homo sapiens	33-39
12917433-8	2003	However, fluorescence measurements of 8-anilino-1-naphthalenesulfonic acid binding to apoE indicated that apoE4 has more exposed hydrophobic surface compared with apoE3 mainly due to the different tertiary organization of the C-terminal domain.	8-anilino-1-naphthalenesulfonic acid	38-74	apolipoprotein E	Homo sapiens	86-90
12917433-8	2003	However, fluorescence measurements of 8-anilino-1-naphthalenesulfonic acid binding to apoE indicated that apoE4 has more exposed hydrophobic surface compared with apoE3 mainly due to the different tertiary organization of the C-terminal domain.	8-anilino-1-naphthalenesulfonic acid	38-74	apolipoprotein E	Homo sapiens	106-111
12646385-13	2003	Studies with the fluorescent probe, 8-anilino-1-naphthalene sulfonic acid, indicated an increase in apoE hydrophobic surface exposure upon decreasing the pH to 3.0.	8-anilino-1-naphthalenesulfonic acid	36-73	apolipoprotein E	Homo sapiens	100-104
12217698-6	2002	In contrast, incubating alpha-synuclein at low pH (pH 4.0 or pH 5.0) resulted in the rapid formation of turbid suspensions characterized by strong ANS binding, reduced thioflavin-T binding and reduced seeding efficiency.	8-anilino-1-naphthalenesulfonic acid	147-150	synuclein alpha	Homo sapiens	24-39
12059774-2	2002	The conformational states of GroEL were monitored by protein intrinsic fluorescence, 8-anilino-1-naphthalene sulfonate fluorescence, and far-UV CD measurements.	8-anilino-1-naphthalenesulfonic acid	85-118	GroEL	Escherichia coli	29-34
12059775-1	2002	Using the hydrophobic fluorescent dye 8-anilino-1-naphthalenesulfonic acid (8-ANS), a hydrophobic site on the surface of the protein globule of angiotensin-converting enzyme (ACE) from bovine lung was found.	8-anilino-1-naphthalenesulfonic acid	38-74	angiotensin I converting enzyme	Bos taurus	144-173
12059775-1	2002	Using the hydrophobic fluorescent dye 8-anilino-1-naphthalenesulfonic acid (8-ANS), a hydrophobic site on the surface of the protein globule of angiotensin-converting enzyme (ACE) from bovine lung was found.	8-anilino-1-naphthalenesulfonic acid	38-74	angiotensin I converting enzyme	Bos taurus	175-178
12059775-1	2002	Using the hydrophobic fluorescent dye 8-anilino-1-naphthalenesulfonic acid (8-ANS), a hydrophobic site on the surface of the protein globule of angiotensin-converting enzyme (ACE) from bovine lung was found.	8-anilino-1-naphthalenesulfonic acid	76-81	angiotensin I converting enzyme	Bos taurus	144-173
12059775-1	2002	Using the hydrophobic fluorescent dye 8-anilino-1-naphthalenesulfonic acid (8-ANS), a hydrophobic site on the surface of the protein globule of angiotensin-converting enzyme (ACE) from bovine lung was found.	8-anilino-1-naphthalenesulfonic acid	76-81	angiotensin I converting enzyme	Bos taurus	175-178
12059775-4	2002	Shielding of the ACE hydrophobic site due to the complex formation with 8-ANS or Triton X-100 resulted in pronounced stabilization of the enzyme against the action of water radiolysis products during gamma-irradiation of dilute solutions of ACE.	8-anilino-1-naphthalenesulfonic acid	72-77	angiotensin I converting enzyme	Bos taurus	17-20
12059775-4	2002	Shielding of the ACE hydrophobic site due to the complex formation with 8-ANS or Triton X-100 resulted in pronounced stabilization of the enzyme against the action of water radiolysis products during gamma-irradiation of dilute solutions of ACE.	8-anilino-1-naphthalenesulfonic acid	72-77	angiotensin I converting enzyme	Bos taurus	241-244
11418618-8	2001	Affinity capillary electrophoresis experiments showed increased specific interactions of the nonnative beta(2)-microglobulin conformation with the dyes 8-anilino-1-naphthalene sulfonic acid and Congo red.	8-anilino-1-naphthalenesulfonic acid	152-189	beta-2-microglobulin	Homo sapiens	103-124
11953433-4	2002	In this study we first show that Bet v 1 in the native state is able to bind the fluorescent probe 8-anilino-1-naphthalenesulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	99-135	delta/notch like EGF repeat containing	Homo sapiens	33-36
11953433-4	2002	In this study we first show that Bet v 1 in the native state is able to bind the fluorescent probe 8-anilino-1-naphthalenesulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	137-140	delta/notch like EGF repeat containing	Homo sapiens	33-36
11953433-5	2002	ANS binds to Bet v 1 with 1:1 stoichiometry, and NMR data indicate that binding takes place in the cavity.	8-anilino-1-naphthalenesulfonic acid	0-3	delta/notch like EGF repeat containing	Homo sapiens	13-16
11432739-9	2001	Similarly, fluorescent dye binding experiments with 8-anilino-1-naphthalene sulfonate revealed increased exposure of apoE3 NT hydrophobic interior as a function of decreasing pH.	8-anilino-1-naphthalenesulfonic acid	52-85	apolipoprotein E	Homo sapiens	117-122
11277996-3	2001	The enhancement of SecA ATPase activity correlated well with the increase in 8-anilino-1-naphthalene-sulfonic acid binding of SecA, suggesting that increased exposure of hydrophobic residues stimulates the enzyme activity.	8-anilino-1-naphthalenesulfonic acid	77-114	ATPase	Escherichia coli	24-30
10924344-3	2000	The glucose dehydrogenase was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	57-94	hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase	Homo sapiens	4-25
10924344-3	2000	The glucose dehydrogenase was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS).	8-anilino-1-naphthalenesulfonic acid	96-99	hexose-6-phosphate dehydrogenase/glucose 1-dehydrogenase	Homo sapiens	4-25
10521270-0	1999	8-anilino-1-naphthalene sulfonic acid (ANS) induces folding of acid unfolded cytochrome c to molten globule state as a result of electrostatic interactions.	8-anilino-1-naphthalenesulfonic acid	39-42	cytochrome c, somatic	Equus caballus	77-89
10521270-2	1999	We have carried out a systematic investigation on the effect of ANS, a charged hydrophobic fluorescent dye, on structural properties of acid-unfolded horse heart cytochrome c at pH 2.0 by a combination of optical methods and electrospray ionization mass spectroscopy (ESI MS).	8-anilino-1-naphthalenesulfonic acid	64-67	cytochrome c, somatic	Equus caballus	162-174
10521270-5	1999	To understand the mechanism of ANS-induced folding of acid-unfolded cytochrome c, comparative ESI MS, soret absorption, and tryptophan fluorescence studies using nile red, a neutral hydrophobic dye, and ANS were carried out.	8-anilino-1-naphthalenesulfonic acid	31-34	cytochrome c, somatic	Equus caballus	68-80
10521270-6	1999	These studies suggested that, at low pH, electrostatic interactions between negatively charged ANS molecules and positively charged amino acid residues present in acid-unfolded cytochrome c are probably responsible for ANS-induced folding of acid-unfolded protein to partially folded compact state or molten globule state.	8-anilino-1-naphthalenesulfonic acid	95-98	cytochrome c, somatic	Equus caballus	177-189
10521270-6	1999	These studies suggested that, at low pH, electrostatic interactions between negatively charged ANS molecules and positively charged amino acid residues present in acid-unfolded cytochrome c are probably responsible for ANS-induced folding of acid-unfolded protein to partially folded compact state or molten globule state.	8-anilino-1-naphthalenesulfonic acid	219-222	cytochrome c, somatic	Equus caballus	177-189
10194351-8	1999	To further probe the molecular organization of lipid-free apoA-I, its effect on the fluorescence properties of 8-anilino-1-naphthalenesulfonic acid (ANS) was determined.	8-anilino-1-naphthalenesulfonic acid	111-147	apolipoprotein AI	Gallus gallus	58-64
10194351-8	1999	To further probe the molecular organization of lipid-free apoA-I, its effect on the fluorescence properties of 8-anilino-1-naphthalenesulfonic acid (ANS) was determined.	8-anilino-1-naphthalenesulfonic acid	149-152	apolipoprotein AI	Gallus gallus	58-64
10194351-9	1999	Human and chicken apoA-I induced a similar increase in ANS fluorescence quantum yield, in keeping with the hypothesis that these proteins adopt a similar global fold in the absence of lipid.	8-anilino-1-naphthalenesulfonic acid	55-58	apolipoprotein AI	Gallus gallus	18-24