PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
9415710-8	1997	Functional regulation of NHE by alpha 1-ARs was determined by alpha 1-AR agonist changes in intracellular pH (pHi) in cells grown on coverslips and loaded with 2",7"-bis(2-carboxyethyl)-5(6)carboxyfluorescein-acetoxymethyl ester.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	160-228	glucose-6-phosphate isomerase 1	Mus musculus	110-113
12126866-3	2002	Here we show that superfusion with 0.1-1 mM valproate induced a moderate intracellular acidification of BCECF-AM-loaded CA3-neurons (hippocampal slices, guinea pig) which was measured as the difference between intracellular pH before (baseline pH(i)) and during valproate treatment (deltapH(i)).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	104-112	carbonic anhydrase 3	Cavia porcellus	120-123
10706990-2	2000	In this study we demonstrate that application of the weak acid propionate (2.5-20 mM) transiently lowers intracellular pH (pH(i)) of BCECF-AM loaded CA3-neurones in hippocampal slices.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	133-141	carbonic anhydrase 3	Homo sapiens	149-152
9776724-7	1998	pHi was measured by epifluorescence with the acetoxymethyl ester form of the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5,6-carboxyfluorescein (BCECF-AM).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	144-152	glucose-6-phosphate isomerase	Homo sapiens	0-3
9260984-4	1997	pHi was determined by fluorescent digital imaging using 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	56-125	glucose-6-phosphate isomerase	Rattus norvegicus	0-3
9260984-4	1997	pHi was determined by fluorescent digital imaging using 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	127-135	glucose-6-phosphate isomerase	Rattus norvegicus	0-3
9087585-2	1997	Muscles were loaded with the fluorescent dye 2"-7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester for simultaneous measurement of pHi and contractility.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	45-114	glucose-6-phosphate isomerase	Homo sapiens	147-150
9232246-4	1997	Using three different assays of cellular toxicity (propidium iodide, BCECF-AM and Trypan Blue), the PAPA complexes caused minimal cellular toxicity.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	69-77	pappalysin 1	Homo sapiens	100-104
8677271-4	1996	pHi in piglet cerebral microvascular endothelial cells in primary culture was monitored using the pH-sensitive fluorescent dye BCECF (2",7"-bis-2-carboxyethyl-5(6)-carboxy-fluorescein acetoxymethyl ester), with dual wavelength fluorescence spectroscopy.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	134-203	glucose-6-phosphate isomerase	Homo sapiens	0-3
7529821-2	1994	The mean intracellular pH in area CA1 of rat hippocampal slices was monitored fluorescently after loading the cells with the dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	129-137	carbonic anhydrase 1	Rattus norvegicus	34-37
7671167-4	1995	Examination of intracellular pH (pHi) using the double excitation of the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) revealed a significantly lower pHi in Hyp/Y mouse osteoblasts compared with +/Y mouse osteoblasts (7.01 +/- 0.03 n = 10 versus 7.15 +/- 0.04 n = 8, respectively; P < 0.05).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	90-159	glucose-6-phosphate isomerase 1	Mus musculus	33-36
7671167-4	1995	Examination of intracellular pH (pHi) using the double excitation of the pH-sensitive dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) revealed a significantly lower pHi in Hyp/Y mouse osteoblasts compared with +/Y mouse osteoblasts (7.01 +/- 0.03 n = 10 versus 7.15 +/- 0.04 n = 8, respectively; P < 0.05).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	90-159	glucose-6-phosphate isomerase 1	Mus musculus	202-205
8082942-2	1994	From each strain Na(+)-H+ exchange activity was determined in lymphocytes using the pH-sensitive fluorescent dye 2",7"-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) by measuring the recovery rate of cytosolic pH (pHi) after intracellular acidification.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	113-182	glucose-6-phosphate isomerase	Rattus norvegicus	242-245
25890268-7	2015	Intracellular pH (pHi) was measured using fluorescent pH-indicator BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	67-75	glucose-6-phosphate isomerase	Homo sapiens	18-21
1328110-3	1992	We used the pH-sensitive fluorescent probe BCECF-AM to measure pHi.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	43-51	glucose-6-phosphate isomerase	Bos taurus	63-66
24213040-3	1989	A linear relationship was established between pHi and the 490/450 fluorescence ratio of BCECF-AM over the pH range 6.0 to 8.0 using the ionophore nigericin.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	88-96	glucose-6-phosphate isomerase	Homo sapiens	46-49
30033048-4	2018	MATERIALS AND METHODS: In current studies, intracellular pH (pHi) and NHE1 activity were analyzed using the pHi-sensitive dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	126-134	solute carrier family 9 member A1	Homo sapiens	70-74
30033048-4	2018	MATERIALS AND METHODS: In current studies, intracellular pH (pHi) and NHE1 activity were analyzed using the pHi-sensitive dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	126-134	glucose-6-phosphate isomerase	Homo sapiens	108-111
29672298-4	2018	METHODS: In this study, we used proton indicator BCECF-AM to analyze the rate of pHi recovery from acidosis in the IH model of rat neonatal cardiomyocytes.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	49-57	glucose-6-phosphate isomerase	Rattus norvegicus	81-84
1742019-2	1991	Using the fluorescent indicator, 2",7"-bis-2-(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester, we report here the first measurements of pHi and buffering power (beta) in single, isolated, uterine smooth muscle cells.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	33-103	glucose-6-phosphate isomerase	Rattus norvegicus	146-149
29995171-5	2018	For fluorometric recordings, the slice-neurons were loaded with the pHi-sensitive dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	86-94	glucose-6-phosphate isomerase	Homo sapiens	68-71
29756391-6	2018	In this study, we used fluorescent microscopy in transiently cultured PASMCs from distal pulmonary arteries of the rat and the pH-sensitive dye, BCECF-AM, to measure changes in resting pHi and NHE activity induced by exposure to exogenous ET-1 (10-8  mol/L) for 24 h. Cell migration and proliferation in response to ET-1 were also measured using Transwell assays and BrdU incorporation, respectively.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	145-153	endothelin 1	Rattus norvegicus	239-243
27035646-5	2016	In the current study, pHi and NHE activity were analyzed using the pHi-sensitive dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	85-93	glucose-6-phosphate isomerase	Homo sapiens	22-25
27035646-5	2016	In the current study, pHi and NHE activity were analyzed using the pHi-sensitive dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	85-93	solute carrier family 9 member C1	Homo sapiens	30-33
27035646-5	2016	In the current study, pHi and NHE activity were analyzed using the pHi-sensitive dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	85-93	glucose-6-phosphate isomerase	Homo sapiens	67-70
26577834-5	2015	The pHi was measured by microspectrofluorimetry with the pH-sensitive dye, BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	75-83	glucose-6-phosphate isomerase	Homo sapiens	4-7
23029469-6	2012	Thus, we used fluorescent microscopy in transiently (3-5 days) cultured rat PASMCs and the pH-sensitive dye, BCECF-AM, to measure changes in basal pH(i) and NHE activity induced by increasing concentrations of ET-1 (10(-10) to 10(-8) M).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	109-117	endothelin 1	Rattus norvegicus	210-214
25047106-10	2014	pH regulation capacity of ae3-/- and WT cardiomyocytes was assessed in cultured cells loaded with the pH-sensitive dye, BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	120-128	solute carrier family 4 (anion exchanger), member 3	Mus musculus	26-29
24454947-4	2014	Using a novel BCECF-AM based assay developed in this study, 8Br-cAMP was shown to decrease the pH of Mct1 vesicles after 25 minutes.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	14-22	solute carrier family 16 member 1	Rattus norvegicus	101-105
25350285-9	2014	Further, AXT3 cells expressing VrNHX1 survived under low pH environment and displayed vacuolar alkalinization analyzed using pH sensitive fluorescent dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	154-162	sodium/hydrogen exchanger 2-like	Vigna radiata	31-37
23080155-5	2012	Using the ratiometric indicators BCECF-AM and fura-2-AM, we found that a hypercapnic stimulus evoked a decrease in pHi and an increase in [Ca(2+)]i.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	33-41	vasoactive intestinal peptide	Danio rerio	115-118
14999730-3	2004	The lipophilic nature of BCECF-AM suggests it may be a substrate for other drug efflux transporters such as P-glycoprotein (P-gp) and the breast cancer resistance protein (BCRP).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	25-33	phosphoglycolate phosphatase	Bos taurus	108-122
15964895-4	2005	pHi was measured using the pH-sensitive fluorescent dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	56-64	glucose-6-phosphate isomerase 1	Mus musculus	0-3
15964895-4	2005	pHi was measured using the pH-sensitive fluorescent dye BCECF-AM.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	56-64	glucose-6-phosphate isomerase 1	Mus musculus	0-2
15796199-3	2005	The rhodamine 123 accumulation of the P-glycoprotein (P-gp)-expressing cells increased up to 46.4, while 2,7"-bis(2-carboxyethyl)-5(6)-carboxy-fluorescein acetoxymethyl ester (BCECF-AM) accumulation of the MRP-expressing cells increased up to 1.6, in fluorescence activity ratio (FAR).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	105-174	ATP binding cassette subfamily C member 1	Homo sapiens	206-209
15796199-3	2005	The rhodamine 123 accumulation of the P-glycoprotein (P-gp)-expressing cells increased up to 46.4, while 2,7"-bis(2-carboxyethyl)-5(6)-carboxy-fluorescein acetoxymethyl ester (BCECF-AM) accumulation of the MRP-expressing cells increased up to 1.6, in fluorescence activity ratio (FAR).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	176-184	ATP binding cassette subfamily B member 1	Homo sapiens	38-52
15796199-3	2005	The rhodamine 123 accumulation of the P-glycoprotein (P-gp)-expressing cells increased up to 46.4, while 2,7"-bis(2-carboxyethyl)-5(6)-carboxy-fluorescein acetoxymethyl ester (BCECF-AM) accumulation of the MRP-expressing cells increased up to 1.6, in fluorescence activity ratio (FAR).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	176-184	ATP binding cassette subfamily B member 1	Homo sapiens	54-58
14999730-3	2004	The lipophilic nature of BCECF-AM suggests it may be a substrate for other drug efflux transporters such as P-glycoprotein (P-gp) and the breast cancer resistance protein (BCRP).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	25-33	phosphoglycolate phosphatase	Bos taurus	124-128
14999730-3	2004	The lipophilic nature of BCECF-AM suggests it may be a substrate for other drug efflux transporters such as P-glycoprotein (P-gp) and the breast cancer resistance protein (BCRP).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	25-33	ATP binding cassette subfamily G member 2	Bos taurus	138-170
14999730-3	2004	The lipophilic nature of BCECF-AM suggests it may be a substrate for other drug efflux transporters such as P-glycoprotein (P-gp) and the breast cancer resistance protein (BCRP).	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	25-33	ATP binding cassette subfamily G member 2	Bos taurus	172-176
14751869-11	2004	In BCECF-AM (10 microm)-loaded cells, both CPA (100 pM-1 microm) and NECA (100 pm-10 microm) elicited concentration-dependent decreases in intracellular pH (pH(i)), with EC(50) values of 3.1+/-0.3 and 6.0+/-0.3 nm, respectively.	2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester	3-11	carboxypeptidase A1	Homo sapiens	43-46