PMID-sentid	Pub_year	Sent_text	compound_name	comp_offset	prot_official_name	organism	prot_offset
18799624-2	2008	Here, we show that increasing the level of myristoylated, alanine-rich C kinase substrate (MARCKS), a prominent substrate of protein kinase C and a phosphatidylinositol-4,5-diphosphate [PI(4,5)P2] sequestration protein highly expressed in the brain, enhanced branching and growth of dendrites both in vitro and in vivo.	pi(4,5)p2	186-195	myristoylated alanine rich protein kinase C substrate	Homo sapiens	43-89
18799624-2	2008	Here, we show that increasing the level of myristoylated, alanine-rich C kinase substrate (MARCKS), a prominent substrate of protein kinase C and a phosphatidylinositol-4,5-diphosphate [PI(4,5)P2] sequestration protein highly expressed in the brain, enhanced branching and growth of dendrites both in vitro and in vivo.	pi(4,5)p2	186-195	myristoylated alanine rich protein kinase C substrate	Homo sapiens	91-97
18545987-9	2008	Acute application of PI(4,5)P2 and a PIP2 scavenger indicates that the mutation N251S renders the kinase PIP5K2A inactive.	pi(4,5)p2	21-30	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	105-112
18574245-1	2008	Phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2) is the endogenous lipid regulating TRPV1.	pi(4,5)p2	41-50	transient receptor potential cation channel subfamily V member 1	Homo sapiens	87-92
18490450-6	2008	In addition, phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) breakdown also leads to the solubilization of EFA6-PH.	pi(4,5)p2	52-61	pleckstrin and Sec7 domain containing	Homo sapiens	109-113
18490450-7	2008	Direct binding measured by surface plasmon resonance gives an apparent affinity of approximately 0.5 microm EFA6-PH for PI(4,5)P2.	pi(4,5)p2	120-129	pleckstrin and Sec7 domain containing	Homo sapiens	108-112
18490450-8	2008	Moreover, we observed in vitro that the catalytic activity of EFA6 is strongly increased by PI(4,5)P2.	pi(4,5)p2	92-101	pleckstrin and Sec7 domain containing	Homo sapiens	62-66
18490450-9	2008	These results indicate that the plasma membrane localization of EFA6-PH is based on its interaction with PI(4,5)P2, and this interaction is necessary for an optimal catalytic activity of EFA6.	pi(4,5)p2	105-114	pleckstrin and Sec7 domain containing	Homo sapiens	64-68
18490450-9	2008	These results indicate that the plasma membrane localization of EFA6-PH is based on its interaction with PI(4,5)P2, and this interaction is necessary for an optimal catalytic activity of EFA6.	pi(4,5)p2	105-114	pleckstrin and Sec7 domain containing	Homo sapiens	187-191
18524949-5	2008	Despite the high degree of identity shared between the active sites of PTEN and Ci-VSP, Ci-VSP dephosphorylates not only the PTEN substrate, phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3], but also, unlike PTEN, phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	259-268	voltage-sensor containing phosphatase	Ciona intestinalis	83-86
18524949-5	2008	Despite the high degree of identity shared between the active sites of PTEN and Ci-VSP, Ci-VSP dephosphorylates not only the PTEN substrate, phosphatidylinositol 3,4,5-trisphosphate [PI(3,4,5)P3], but also, unlike PTEN, phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	259-268	voltage-sensor containing phosphatase	Ciona intestinalis	91-94
18524949-8	2008	Ci-VSP with a G365A mutation no longer dephosphorylates PI(4,5)P2 and is not capable of inducing depolarization-dependent rundown of a PI(4,5)P2-dependent potassium channel.	pi(4,5)p2	56-65	voltage-sensor containing phosphatase	Ciona intestinalis	3-6
18524949-9	2008	These results indicate that Ci-VSP is a PI(3,4,5)P3/PI(4,5)P2 phosphatase that uniquely functions in the voltage-dependent regulation of ion channels through regulation of PI(4,5)P2 levels.	pi(4,5)p2	52-61	voltage-sensor containing phosphatase	Ciona intestinalis	31-34
18220422-4	2008	Several independent studies reported that PI(4,5)P2 enhances PTEN phosphatase activity, but the reasons for this enhancement are currently being debated.	pi(4,5)p2	42-51	phosphatase and tensin homolog	Homo sapiens	61-65
18220422-5	2008	In this study, PTEN bound to PI(4,5)P2-bearing vesicles has increased alpha-helicity, providing direct spectroscopic proof of a conformational change.	pi(4,5)p2	29-38	phosphatase and tensin homolog	Homo sapiens	15-19
18220422-7	2008	On the basis of experiments with two mutant PTEN proteins, it is shown that PI(4,5)P2 induces this conformational change by binding to the PTEN N-terminal domain.	pi(4,5)p2	76-85	phosphatase and tensin homolog	Homo sapiens	44-48
18220422-7	2008	On the basis of experiments with two mutant PTEN proteins, it is shown that PI(4,5)P2 induces this conformational change by binding to the PTEN N-terminal domain.	pi(4,5)p2	76-85	phosphatase and tensin homolog	Homo sapiens	139-143
18220422-8	2008	Using PTEN protein and a 21-amino acid peptide based on the PTEN N-terminus, we tested all natural phosphatidylinositol phosphates and found preferential binding of PI(4,5)P2.	pi(4,5)p2	165-174	phosphatase and tensin homolog	Homo sapiens	6-10
17890681-1	2007	Previously, ceramide-1-phosphate (C1P) and phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] were demonstrated to be potent and specific activators of group IVA cytosolic phospholipase A2 (cPLA2alpha).	pi(4,5)p2	82-91	phospholipase A2 group IVA	Homo sapiens	161-187
18220422-8	2008	Using PTEN protein and a 21-amino acid peptide based on the PTEN N-terminus, we tested all natural phosphatidylinositol phosphates and found preferential binding of PI(4,5)P2.	pi(4,5)p2	165-174	phosphatase and tensin homolog	Homo sapiens	60-64
17890681-1	2007	Previously, ceramide-1-phosphate (C1P) and phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] were demonstrated to be potent and specific activators of group IVA cytosolic phospholipase A2 (cPLA2alpha).	pi(4,5)p2	82-91	phospholipase A2 group IVA	Homo sapiens	189-199
17890681-3	2007	Indeed, surface plasmon resonance and surface dilution kinetics demonstrated that C1P was a more potent effector than PI(4,5)P2 in decreasing the dissociation constant of the cPLA2alpha-phosphatidylcholine (PC) interaction and increasing the residence time of the enzyme on the vesicles/micelles.	pi(4,5)p2	118-127	phospholipase A2 group IVA	Homo sapiens	175-185
17890681-5	2007	Furthermore, PI(4,5)P2 activated cPLA2alpha with a stoichiometry of 1:1 versus C1P at 2.4:1.	pi(4,5)p2	13-22	phospholipase A2 group IVA	Homo sapiens	33-43
17890681-6	2007	Lastly, PI(4,5)P2, but not C1P, increased the penetration ability of cPLA2alpha into PC-rich membranes.	pi(4,5)p2	8-17	phospholipase A2 group IVA	Homo sapiens	69-79
17538019-2	2007	We found that phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) was generated at sites of N-cadherin-mediated intercellular adhesion and was a critical regulator of intercellular adhesion strength.	pi(4,5)p2	53-62	cadherin 2	Homo sapiens	90-100
17825837-0	2007	pH-dependent binding of the Epsin ENTH domain and the AP180 ANTH domain to PI(4,5)P2-containing bilayers.	pi(4,5)p2	75-84	clathrin interactor 1	Homo sapiens	34-38
17825837-0	2007	pH-dependent binding of the Epsin ENTH domain and the AP180 ANTH domain to PI(4,5)P2-containing bilayers.	pi(4,5)p2	75-84	synaptosome associated protein 91	Homo sapiens	54-59
17538019-3	2007	Immunostaining for PI(4,5)P2 or transfection with GFP-PH-PLCdelta showed that PI(4,5)P2 was enriched at sites of N-cadherin adhesions and this enrichment required activated Rac1.	pi(4,5)p2	78-87	cadherin 2	Homo sapiens	113-123
17538019-3	2007	Immunostaining for PI(4,5)P2 or transfection with GFP-PH-PLCdelta showed that PI(4,5)P2 was enriched at sites of N-cadherin adhesions and this enrichment required activated Rac1.	pi(4,5)p2	78-87	Rac family small GTPase 1	Homo sapiens	173-177
17538019-7	2007	Catalytically inactive PIP5KIgamma or a cell-permeant peptide that mimics and competes for the PI(4,5)P2-binding region of the actin-binding protein gelsolin inhibited incorporation of actin monomers in response to N-cadherin ligation and reduced intercellular adhesion strength by more than twofold.	pi(4,5)p2	95-104	gelsolin	Homo sapiens	149-157
17538019-7	2007	Catalytically inactive PIP5KIgamma or a cell-permeant peptide that mimics and competes for the PI(4,5)P2-binding region of the actin-binding protein gelsolin inhibited incorporation of actin monomers in response to N-cadherin ligation and reduced intercellular adhesion strength by more than twofold.	pi(4,5)p2	95-104	cadherin 2	Homo sapiens	215-225
17538019-8	2007	Gelsolin null fibroblasts transfected with a gelsolin severing mutant containing an intact PI(4,5)P2 binding region, demonstrated intercellular adhesion strength similar to wild-type transfected controls.	pi(4,5)p2	91-100	gelsolin	Homo sapiens	0-8
17538019-8	2007	Gelsolin null fibroblasts transfected with a gelsolin severing mutant containing an intact PI(4,5)P2 binding region, demonstrated intercellular adhesion strength similar to wild-type transfected controls.	pi(4,5)p2	91-100	gelsolin	Homo sapiens	45-53
17538019-9	2007	We conclude that PIP5KIgamma-mediated generation of PI(4,5)P2 at sites of N-cadherin contacts regulates intercellular adhesion strength, an effect due in part to PI(4,5)P2-mediated regulation of gelsolin.	pi(4,5)p2	52-61	cadherin 2	Homo sapiens	74-84
17538019-9	2007	We conclude that PIP5KIgamma-mediated generation of PI(4,5)P2 at sites of N-cadherin contacts regulates intercellular adhesion strength, an effect due in part to PI(4,5)P2-mediated regulation of gelsolin.	pi(4,5)p2	52-61	gelsolin	Homo sapiens	195-203
17538019-9	2007	We conclude that PIP5KIgamma-mediated generation of PI(4,5)P2 at sites of N-cadherin contacts regulates intercellular adhesion strength, an effect due in part to PI(4,5)P2-mediated regulation of gelsolin.	pi(4,5)p2	162-171	cadherin 2	Homo sapiens	74-84
17538019-9	2007	We conclude that PIP5KIgamma-mediated generation of PI(4,5)P2 at sites of N-cadherin contacts regulates intercellular adhesion strength, an effect due in part to PI(4,5)P2-mediated regulation of gelsolin.	pi(4,5)p2	162-171	gelsolin	Homo sapiens	195-203
17371834-0	2007	Missing-in-metastasis and IRSp53 deform PI(4,5)P2-rich membranes by an inverse BAR domain-like mechanism.	pi(4,5)p2	40-49	BAR/IMD domain containing adaptor protein 2	Homo sapiens	26-32
17302440-0	2007	Differential regulation of WASP and N-WASP by Cdc42, Rac1, Nck, and PI(4,5)P2.	pi(4,5)p2	68-77	WASP actin nucleation promoting factor	Homo sapiens	27-31
17302440-0	2007	Differential regulation of WASP and N-WASP by Cdc42, Rac1, Nck, and PI(4,5)P2.	pi(4,5)p2	68-77	WASP like actin nucleation promoting factor	Homo sapiens	36-42
17290217-4	2007	We demonstrate here that AP-2 complex directly interacts with phosphatidylinositol 4-phosphate 5-kinase gamma661 (PIP5Kgamma661), the major PI(4,5)P2-producing enzyme in the brain.	pi(4,5)p2	140-149	transcription factor AP-2, alpha	Mus musculus	25-29
16799990-5	2006	Treatment with PI(4,5)P2 or a phospholipase C inhibitor, U73122, resulted in decreased mRNA expressions of albumin and hepatocyte nuclear factor 4 (HNF-4) in hepatocytes.	pi(4,5)p2	15-24	hepatocyte nuclear factor 4, alpha	Rattus norvegicus	119-146
17254962-2	2007	Using a three-dimensional model of epithelial morphogenesis, report that the phosphatase PTEN and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] regulate the GTPase Cdc42 and the kinase aPKC to generate the apical plasma membrane domain and maintain apical-basolateral polarity.	pi(4,5)p2	139-148	cell division cycle 42	Homo sapiens	170-175
16842970-3	2007	PTEN antagonises PI3K by degrading PIP3 to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	82-91	phosphatase and tensin homolog	Homo sapiens	0-4
16888807-10	2007	These results suggest a model in which mPIP5K-Ibeta is surrounded by PI(4)P, but is unable to catalyze its conversion to PI(4,5)P2 unless PA is bound.	pi(4,5)p2	121-130	phosphatidylinositol-4-phosphate 5-kinase, type 1 beta	Mus musculus	39-51
16801950-6	2006	PIP5K2A is involved in the biosynthesis of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), one of the key metabolic crossroads in phosphoinositide signalling.	pi(4,5)p2	82-91	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	0-7
16669615-1	2006	Phospholipase Cbeta (PLCbeta) enzymes are activated by Galpha q and Gbetagamma subunits and catalyze the hydrolysis of the minor membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	183-192	G protein subunit alpha q	Homo sapiens	55-63
16799990-5	2006	Treatment with PI(4,5)P2 or a phospholipase C inhibitor, U73122, resulted in decreased mRNA expressions of albumin and hepatocyte nuclear factor 4 (HNF-4) in hepatocytes.	pi(4,5)p2	15-24	hepatocyte nuclear factor 4, alpha	Rattus norvegicus	148-153
16566581-4	2006	In particular, the NHS-biotin modification approach was used to identify the lysine residues that are exposed to the solvent in free Gag and are protected from biotinylation by direct protein-ligand or protein-protein contacts in Gag complexes with PI(4,5)P2 and/or RNA.	pi(4,5)p2	249-258	Pr55(Gag)	Human immunodeficiency virus 1	230-233
16460838-3	2006	Interaction of PI(4,5)P2 with gelsolin and Wiscott-Aldrich syndrome protein (WASP) is critical for podosome assembly/disassembly and actin ring formation in osteoclasts.	pi(4,5)p2	15-24	gelsolin	Mus musculus	30-38
16460838-3	2006	Interaction of PI(4,5)P2 with gelsolin and Wiscott-Aldrich syndrome protein (WASP) is critical for podosome assembly/disassembly and actin ring formation in osteoclasts.	pi(4,5)p2	15-24	Wiskott-Aldrich syndrome	Mus musculus	43-75
16460838-3	2006	Interaction of PI(4,5)P2 with gelsolin and Wiscott-Aldrich syndrome protein (WASP) is critical for podosome assembly/disassembly and actin ring formation in osteoclasts.	pi(4,5)p2	15-24	Wiskott-Aldrich syndrome	Mus musculus	77-81
15870270-3	2005	Here we show that the LIM protein Ajuba contributes to the cellular regulation of PI(4,5)P2 levels by interacting with and activating the enzymatic activity of the PI(4)P 5-kinase (PIPKIalpha), the predominant enzyme in the synthesis of PI(4,5)P2, in a migration stimulus-regulated manner.	pi(4,5)p2	82-91	PDZ and LIM domain 5	Mus musculus	22-25
16476663-1	2006	An essential step in Drosophila phototransduction is the hydrolysis of phosphatidylinositol 4,5 bisphosphate PI(4,5)P2 by phospholipase Cbeta (PLCbeta) to generate a second messenger that opens the light-activated channels TRP and TRPL.	pi(4,5)p2	109-118	no receptor potential A	Drosophila melanogaster	122-141
16476663-1	2006	An essential step in Drosophila phototransduction is the hydrolysis of phosphatidylinositol 4,5 bisphosphate PI(4,5)P2 by phospholipase Cbeta (PLCbeta) to generate a second messenger that opens the light-activated channels TRP and TRPL.	pi(4,5)p2	109-118	no receptor potential A	Drosophila melanogaster	143-150
16186103-8	2005	Infusion with p110beta/p85alpha or p110gamma PI3K in the presence of PI(4,5)P2 also restored I(Ca,L) density to wild-type levels.	pi(4,5)p2	69-78	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta	Mus musculus	14-22
16186103-8	2005	Infusion with p110beta/p85alpha or p110gamma PI3K in the presence of PI(4,5)P2 also restored I(Ca,L) density to wild-type levels.	pi(4,5)p2	69-78	phosphoinositide-3-kinase regulatory subunit 1	Mus musculus	23-31
16186103-8	2005	Infusion with p110beta/p85alpha or p110gamma PI3K in the presence of PI(4,5)P2 also restored I(Ca,L) density to wild-type levels.	pi(4,5)p2	69-78	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma	Mus musculus	35-44
16120646-3	2005	We determined here whether phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] regulation of gelsolin is required for collagen internalization.	pi(4,5)p2	66-75	gelsolin	Homo sapiens	91-99
16120646-5	2005	PI(4,5)P2 accumulated around collagen during internalization and was associated with gelsolin.	pi(4,5)p2	0-9	gelsolin	Homo sapiens	85-93
16120646-9	2005	We conclude that separate functions of gelsolin mediate sequential stages of collagen phagocytosis: Ca2+-dependent actin severing facilitates collagen binding, whereas PI(4,5)P2-dependent regulation of gelsolin promotes the actin assembly required for internalization of collagen fibrils.	pi(4,5)p2	168-177	gelsolin	Homo sapiens	39-47
16120646-9	2005	We conclude that separate functions of gelsolin mediate sequential stages of collagen phagocytosis: Ca2+-dependent actin severing facilitates collagen binding, whereas PI(4,5)P2-dependent regulation of gelsolin promotes the actin assembly required for internalization of collagen fibrils.	pi(4,5)p2	168-177	gelsolin	Homo sapiens	202-210
15790568-5	2005	In the present structure-function analysis, deletion of EF1 and EF2 of the N-terminal four EF-hand domains caused marked reduction of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2)-hydrolyzing activity in vitro and loss of Ca2+ oscillation-inducing activity in mouse eggs after injection of RNA encoding the mutant.	pi(4,5)p2	173-182	natriuretic peptide receptor 3	Mus musculus	64-67
16472657-0	2006	Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts.	pi(4,5)p2	10-19	actin like 6A S homeolog	Xenopus laevis	28-33
16472657-3	2006	We have developed methods to study Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts.	pi(4,5)p2	45-54	actin like 6A S homeolog	Xenopus laevis	63-68
16472657-4	2006	In this chapter, we describe detailed procedures to prepare Xenopus egg extracts, Cdc42, and PI(4,5)P2 for use in actin assembly experiments.	pi(4,5)p2	93-102	actin like 6A S homeolog	Xenopus laevis	114-119
15870270-3	2005	Here we show that the LIM protein Ajuba contributes to the cellular regulation of PI(4,5)P2 levels by interacting with and activating the enzymatic activity of the PI(4)P 5-kinase (PIPKIalpha), the predominant enzyme in the synthesis of PI(4,5)P2, in a migration stimulus-regulated manner.	pi(4,5)p2	82-91	ajuba LIM protein	Mus musculus	34-39
15870270-3	2005	Here we show that the LIM protein Ajuba contributes to the cellular regulation of PI(4,5)P2 levels by interacting with and activating the enzymatic activity of the PI(4)P 5-kinase (PIPKIalpha), the predominant enzyme in the synthesis of PI(4,5)P2, in a migration stimulus-regulated manner.	pi(4,5)p2	237-246	PDZ and LIM domain 5	Mus musculus	22-25
15574849-5	2004	Our biochemical studies reveal that although both At5PTase12 and At5PTase13 exhibit phosphatase activity toward only Ins(1,4,5)P3, At5PTase14 hydrolyzes phosphate from PI(4,5)P2, PI(3,4,5)P3 and Ins(1,4,5)P3 with the highest substrate affinity toward PI(4,5)P2.	pi(4,5)p2	168-177	Endonuclease/exonuclease/phosphatase family protein	Arabidopsis thaliana	65-75
15870270-3	2005	Here we show that the LIM protein Ajuba contributes to the cellular regulation of PI(4,5)P2 levels by interacting with and activating the enzymatic activity of the PI(4)P 5-kinase (PIPKIalpha), the predominant enzyme in the synthesis of PI(4,5)P2, in a migration stimulus-regulated manner.	pi(4,5)p2	237-246	ajuba LIM protein	Mus musculus	34-39
15870270-5	2005	Reintroduction of Ajuba into these cells normalized PI(4,5)P2 levels.	pi(4,5)p2	52-61	ajuba LIM protein	Mus musculus	18-23
15870270-6	2005	Localization of PI(4,5)P2 synthesis and PIPKIalpha in the leading lamellipodia and membrane ruffles, respectively, of migrating Ajuba(-/-) MEFs was impaired.	pi(4,5)p2	16-25	ajuba LIM protein	Mus musculus	128-133
15870270-8	2005	Thus, in addition to its effects upon Rac activity Ajuba can also influence cell migration through regulation of PI(4,5)P2 synthesis through direct activation of PIPKIalpha enzyme activity.	pi(4,5)p2	113-122	ajuba LIM protein	Mus musculus	51-56
15758165-7	2005	Expression of a membrane-targeted inositol 5-phosphatase domain of synaptojanin 1 eliminated PI(4,5)P2 from the membrane and abolished secretion.	pi(4,5)p2	93-102	synaptojanin 1	Bos taurus	67-81
15852009-0	2005	PI(4,5)P2 regulates the activation and desensitization of TRPM8 channels through the TRP domain.	pi(4,5)p2	0-9	transient receptor potential cation channel subfamily M member 8	Homo sapiens	58-63
15738269-1	2005	The interaction of talin with phosphatidylinositol(4) phosphate 5 kinase type I gamma (PIPKI gamma) regulates PI(4,5)P2 synthesis at synapses and at focal adhesions.	pi(4,5)p2	110-119	phosphatidylinositol-4-phosphate 5-kinase type 1 gamma	Homo sapiens	30-85
15738269-1	2005	The interaction of talin with phosphatidylinositol(4) phosphate 5 kinase type I gamma (PIPKI gamma) regulates PI(4,5)P2 synthesis at synapses and at focal adhesions.	pi(4,5)p2	110-119	phosphatidylinositol-4-phosphate 5-kinase type 1 gamma	Homo sapiens	87-98
15465916-8	2004	Strikingly, in cells expressing Arf6/Q67L, Gag was redirected to the PI(4,5)P2-enriched vesicles and HIV-1 virions budded into these vesicles.	pi(4,5)p2	69-78	ADP ribosylation factor 6	Homo sapiens	32-36
15465916-8	2004	Strikingly, in cells expressing Arf6/Q67L, Gag was redirected to the PI(4,5)P2-enriched vesicles and HIV-1 virions budded into these vesicles.	pi(4,5)p2	69-78	Pr55(Gag)	Human immunodeficiency virus 1	43-46
15465916-9	2004	These results demonstrate that PI(4,5)P2 plays a key role in Gag targeting to the plasma membrane and thus serves as a cellular determinant of HIV-1 particle production.	pi(4,5)p2	31-40	Pr55(Gag)	Human immunodeficiency virus 1	61-64
14585928-5	2003	ARF6(T27N) led to a depolarization-dependent reduction in the levels of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] with a time course that paralleled the inhibition of secretion.	pi(4,5)p2	111-120	ADP-ribosylation factor 6	Mus musculus	0-4
14764604-3	2004	Our data suggest that a phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) binding motif at the N terminus of PTEN serves the dual role of localizing the enzyme to the membrane and regulating its activity.	pi(4,5)p2	63-72	phosphatase and tensin homolog	Homo sapiens	109-113
15041641-4	2004	Here, we use the finite difference Poisson-Boltzmann method to test this hypothesis by calculating the electrostatic free energy of lateral sequestration of PI(4,5)P2 by membrane-adsorbed basic peptides: Lys-7, Lys-13, and FA-MARCKS(151-175), a peptide based on MARCKS(151-175).	pi(4,5)p2	157-166	myristoylated alanine rich protein kinase C substrate	Homo sapiens	226-232
15041641-4	2004	Here, we use the finite difference Poisson-Boltzmann method to test this hypothesis by calculating the electrostatic free energy of lateral sequestration of PI(4,5)P2 by membrane-adsorbed basic peptides: Lys-7, Lys-13, and FA-MARCKS(151-175), a peptide based on MARCKS(151-175).	pi(4,5)p2	157-166	myristoylated alanine rich protein kinase C substrate	Homo sapiens	262-268
14585928-7	2003	These results indicate that ARF6 is required to sustain adequate levels of PI(4,5)P2 during periods of increased PI(4,5)P2 metabolism such as regulated secretion.	pi(4,5)p2	75-84	ADP-ribosylation factor 6	Mus musculus	28-32
14585928-7	2003	These results indicate that ARF6 is required to sustain adequate levels of PI(4,5)P2 during periods of increased PI(4,5)P2 metabolism such as regulated secretion.	pi(4,5)p2	113-122	ADP-ribosylation factor 6	Mus musculus	28-32
12857747-8	2003	Hence, the interaction between PI(4,5)P2 and PTEN requires specific, ionic interactions with the phosphate groups on the inositol ring as well as hydrophobic interactions with the fatty acid chains, likely mimicking the physiological interactions that PTEN has with the polar surface head groups and the hydrophobic core of phospholipid membranes.	pi(4,5)p2	31-40	phosphatase and tensin homolog	Homo sapiens	45-49
12857747-11	2003	These results suggest a model in which a PI(4,5)P2 monomer binds to PTEN, initiates an allosteric conformational change and, thereby, activates PTEN independent of membrane binding.	pi(4,5)p2	41-50	phosphatase and tensin homolog	Homo sapiens	144-148
12857747-3	2003	Binding of di-C8-phosphatidylinositol 4,5-P2 (PI(4,5)P2) to PTEN enhances phosphatase activity for monodispersed substrates, PI(3,4,5)P3 and PI(3,4)P2.	pi(4,5)p2	46-55	phosphatase and tensin homolog	Homo sapiens	60-64
12857747-3	2003	Binding of di-C8-phosphatidylinositol 4,5-P2 (PI(4,5)P2) to PTEN enhances phosphatase activity for monodispersed substrates, PI(3,4,5)P3 and PI(3,4)P2.	pi(4,5)p2	46-55	peptidase inhibitor 3	Homo sapiens	141-150
12857747-5	2003	Activation by exogenous PI(4,5)P2 is more apparent with PI(3,4)P2 as a substrate than with PI(3,4,5)P3, probably because hydrolysis of PI(3,4)P2 yields PI(4)P, which is not an activator.	pi(4,5)p2	24-33	peptidase inhibitor 3	Homo sapiens	56-65
12857747-5	2003	Activation by exogenous PI(4,5)P2 is more apparent with PI(3,4)P2 as a substrate than with PI(3,4,5)P3, probably because hydrolysis of PI(3,4)P2 yields PI(4)P, which is not an activator.	pi(4,5)p2	24-33	peptidase inhibitor 3	Homo sapiens	135-144
12857747-8	2003	Hence, the interaction between PI(4,5)P2 and PTEN requires specific, ionic interactions with the phosphate groups on the inositol ring as well as hydrophobic interactions with the fatty acid chains, likely mimicking the physiological interactions that PTEN has with the polar surface head groups and the hydrophobic core of phospholipid membranes.	pi(4,5)p2	31-40	phosphatase and tensin homolog	Homo sapiens	252-256
12857747-11	2003	These results suggest a model in which a PI(4,5)P2 monomer binds to PTEN, initiates an allosteric conformational change and, thereby, activates PTEN independent of membrane binding.	pi(4,5)p2	41-50	phosphatase and tensin homolog	Homo sapiens	68-72
12670959-2	2003	Both the MARCKS protein and a peptide corresponding to the effector domain (an unstructured region that contains 13 basic residues and 5 phenylalanines), MARCKS-(151-175), laterally sequester the polyvalent lipid PI(4,5)P2 in the plane of a bilayer membrane with high affinity.	pi(4,5)p2	213-222	myristoylated alanine rich protein kinase C substrate	Homo sapiens	9-15
12670959-2	2003	Both the MARCKS protein and a peptide corresponding to the effector domain (an unstructured region that contains 13 basic residues and 5 phenylalanines), MARCKS-(151-175), laterally sequester the polyvalent lipid PI(4,5)P2 in the plane of a bilayer membrane with high affinity.	pi(4,5)p2	213-222	myristoylated alanine rich protein kinase C substrate	Homo sapiens	154-160
12670959-4	2003	Measurements of cross-relaxation rates in two-dimensional nuclear Overhauser enhancement spectroscopy NMR experiments show that the five Phe rings of MARCKS-(151-175) penetrate into the acyl chain region of phosphatidylcholine bilayers containing phosphatidylglycerol or PI(4,5)P2.	pi(4,5)p2	271-280	myristoylated alanine rich protein kinase C substrate	Homo sapiens	150-156
12670959-7	2003	The deep location of the MARCKS peptide in the polar head group region should enhance its electrostatic sequestration of PI(4,5)P2 by an "image charge" mechanism.	pi(4,5)p2	121-130	myristoylated alanine rich protein kinase C substrate	Homo sapiens	25-31
12486109-4	2002	We report that mutation of the PH domains of yeast and mammalian PLD enzymes generates catalytically active PI(4,5)P2-regulated enzymes with impaired biological functions.	pi(4,5)p2	108-117	glycosylphosphatidylinositol specific phospholipase D1	Homo sapiens	65-68
12670959-1	2003	Electrostatic interactions with positively charged regions of membrane-associated proteins such as myristoylated alanine-rich C kinase substrate (MARCKS) may have a role in regulating the level of free phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) in plasma membranes.	pi(4,5)p2	241-250	myristoylated alanine rich protein kinase C substrate	Homo sapiens	99-144
12670959-1	2003	Electrostatic interactions with positively charged regions of membrane-associated proteins such as myristoylated alanine-rich C kinase substrate (MARCKS) may have a role in regulating the level of free phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) in plasma membranes.	pi(4,5)p2	241-250	myristoylated alanine rich protein kinase C substrate	Homo sapiens	146-152
12486109-5	2002	Disruption of the PH domain of mammalian PLD2 results in relocalization of the protein from the PI(4,5)P2-containing plasma membrane to endosomes.	pi(4,5)p2	96-105	phospholipase D2	Homo sapiens	41-45
12486109-9	2002	Studies with PLD2-derived peptides suggest that this binding involves a previously identified polybasic motif that mediates activation of the enzyme by PI(4,5)P2.	pi(4,5)p2	152-161	phospholipase D2	Homo sapiens	13-17
12486109-10	2002	By comparison, the PLD2 PH domain binds PI(4,5)P2 with lower affinity but sufficient selectivity to function in concert with the polybasic motif to target the protein to PI(4,5)P2-rich membranes.	pi(4,5)p2	40-49	phospholipase D2	Homo sapiens	19-23
12486109-10	2002	By comparison, the PLD2 PH domain binds PI(4,5)P2 with lower affinity but sufficient selectivity to function in concert with the polybasic motif to target the protein to PI(4,5)P2-rich membranes.	pi(4,5)p2	170-179	phospholipase D2	Homo sapiens	19-23
12492148-3	2002	Binding of the fluorescent dynamin II to giant unilamellar vesicles, in the presence and absence of PI(4,5)P2, was directly observed using two-photon fluorescence microscopy.	pi(4,5)p2	100-109	dynamin 2	Homo sapiens	27-37
12119359-3	2002	Here we have identified and functionally characterized a conserved binding site for PI(4,5)P2 within mu2-adaptin, the medium chain of the clathrin adaptor complex AP-2.	pi(4,5)p2	84-93	adaptor related protein complex 1 subunit mu 2	Homo sapiens	101-104
12119359-3	2002	Here we have identified and functionally characterized a conserved binding site for PI(4,5)P2 within mu2-adaptin, the medium chain of the clathrin adaptor complex AP-2.	pi(4,5)p2	84-93	transcription factor AP-2 alpha	Homo sapiens	163-167
12119359-4	2002	Mutant mu2 lacking a cluster of conserved lysine residues fails to bind PI(4,5)P2 and to compete the recruitment of native clathrin/AP-2 to PI(4,5)P2-containing liposomes or to presynaptic membranes.	pi(4,5)p2	72-81	adaptor related protein complex 1 subunit mu 2	Homo sapiens	7-10
12119359-4	2002	Mutant mu2 lacking a cluster of conserved lysine residues fails to bind PI(4,5)P2 and to compete the recruitment of native clathrin/AP-2 to PI(4,5)P2-containing liposomes or to presynaptic membranes.	pi(4,5)p2	140-149	adaptor related protein complex 1 subunit mu 2	Homo sapiens	7-10
12119359-6	2002	We suggest that PI(4,5)P2 binding to mu2-adaptin regulates clathrin-mediated endocytosis and thereby may contribute to structurally linking cargo recognition to coat formation.	pi(4,5)p2	16-25	adaptor related protein complex 1 subunit mu 2	Homo sapiens	37-40
9624178-4	1998	Cells overexpressing MSS4 contain an elevated level specifically of PI(4,5)P2, whereas mss4 mutant cells have only approximately 10% of the normal amount of this phosphorylated phosphoinositide.	pi(4,5)p2	68-77	1-phosphatidylinositol-4-phosphate 5-kinase	Saccharomyces cerevisiae S288C	21-25
11273133-5	2000	Particle motility induced by ARF6 involved Arp2/3 complex, tyrosine kinase activity, phospholipase D (PLD) and D3-phosphoinositides, but not phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	180-189	ADP ribosylation factor 6	Homo sapiens	29-33
10589680-1	1999	Synthesis of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a signaling phospholipid, is primarily carried out by phosphatidylinositol 4-phosphate 5-kinase [PI(4)P5K], which has been reported to be regulated by RhoA and Rac1.	pi(4,5)p2	52-61	ras homolog family member A	Homo sapiens	215-219
10589680-1	1999	Synthesis of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a signaling phospholipid, is primarily carried out by phosphatidylinositol 4-phosphate 5-kinase [PI(4)P5K], which has been reported to be regulated by RhoA and Rac1.	pi(4,5)p2	52-61	Rac family small GTPase 1	Homo sapiens	224-228
10589680-5	1999	PLD2 similarly translocates to the ruffles, as does the PH domain of phospholipase Cdelta1, indicating locally elevated PI(4,5)P2.	pi(4,5)p2	120-129	phospholipase D2	Homo sapiens	0-4
10545132-11	1999	Three synthetic isoforms of PIP2, PI(4,5)P2, PI(3,4)P2 and PI(3,5)P2, activated GIRK channels with similar potencies.	pi(4,5)p2	34-43	potassium inwardly rectifying channel subfamily J member 3 S homeolog	Xenopus laevis	80-84
11926815-9	2002	Circular dichroism spectroscopy revealed a conformational change caused by PI(4,5)P2 binding to the catalytic region of PLD.	pi(4,5)p2	75-84	glycosylphosphatidylinositol specific phospholipase D1	Homo sapiens	120-123
12224553-4	2002	Additionally, we will discuss how phosphoinositides, such as PI(4,5)P2, small GTPases thought to control the actin cytoskeleton, such as Rho, Rac, and Cdc42, or membrane trafficking, such as Rab GTPases and ARF proteins, and different kinases may participate in the functional connection of actin and endocytosis.	pi(4,5)p2	61-70	cell division cycle 42	Homo sapiens	151-156
11747431-6	2001	We also show that yeast cofilin can differentiate between various phosphatidylinositides, and mapped the PI(4,5)P2 binding site by using a collection of cofilin mutants.	pi(4,5)p2	105-114	cofilin	Saccharomyces cerevisiae S288C	24-31
11747431-6	2001	We also show that yeast cofilin can differentiate between various phosphatidylinositides, and mapped the PI(4,5)P2 binding site by using a collection of cofilin mutants.	pi(4,5)p2	105-114	cofilin	Saccharomyces cerevisiae S288C	153-160
11747431-9	2001	The PI(4,5)P2-binding site overlaps with areas that are important for F-actin binding, explaining why the actin-related activities of cofilin/ADF are inhibited by PI(4,5)P2.	pi(4,5)p2	4-13	cofilin	Saccharomyces cerevisiae S288C	134-145
11747431-10	2001	The biological roles of actin and PI(4,5)P2 interactions of cofilin are discussed in light of phenotypes of specific yeast strains carrying mutations in residues that are important for actin and PI(4,5)P2 binding.	pi(4,5)p2	34-43	cofilin	Saccharomyces cerevisiae S288C	60-67
11747431-10	2001	The biological roles of actin and PI(4,5)P2 interactions of cofilin are discussed in light of phenotypes of specific yeast strains carrying mutations in residues that are important for actin and PI(4,5)P2 binding.	pi(4,5)p2	195-204	cofilin	Saccharomyces cerevisiae S288C	60-67
11401853-5	2001	These data indicate that PI(4,5)P2 synthesis rates and other parameters of CCh-stimulated inositol phospholipid turnover are muscle length-dependent and provide evidence that supports the hypothesis that length-dependent beta1-integrin signals may exert control on CCh-activated PI(4,5)P2 synthesis.	pi(4,5)p2	25-34	integrin subunit beta 1	Homo sapiens	221-235
11401853-5	2001	These data indicate that PI(4,5)P2 synthesis rates and other parameters of CCh-stimulated inositol phospholipid turnover are muscle length-dependent and provide evidence that supports the hypothesis that length-dependent beta1-integrin signals may exert control on CCh-activated PI(4,5)P2 synthesis.	pi(4,5)p2	279-288	integrin subunit beta 1	Homo sapiens	221-235
10660303-3	2000	The activities of both PLD isoforms are dependent on phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and our sequence analysis suggested the presence of a pleckstrin homology (PH) domain in PLD1, although its absence has also been daimed.	pi(4,5)p2	92-101	glycosylphosphatidylinositol specific phospholipase D1	Homo sapiens	23-26
10660303-5	2000	Furthermore, PLD1 bound specifically and with high affinity to lipid surfaces containing PI(4,5)P2 independently of the substrate phosphatidylcholine, suggesting a key role for the PH domain in PLD function.	pi(4,5)p2	89-98	phospholipase D1	Homo sapiens	13-17
10660303-5	2000	Furthermore, PLD1 bound specifically and with high affinity to lipid surfaces containing PI(4,5)P2 independently of the substrate phosphatidylcholine, suggesting a key role for the PH domain in PLD function.	pi(4,5)p2	89-98	glycosylphosphatidylinositol specific phospholipase D1	Homo sapiens	13-16
10660303-6	2000	Importantly, a glutathione-S-transferase (GST) fusion protein comprising GST and the PH domain of PLD1 (GST-PLD1-PH) also bound specifically to supported lipid monolayers containing PI(4,5)P2.	pi(4,5)p2	182-191	phospholipase D1	Homo sapiens	98-102
10660303-6	2000	Importantly, a glutathione-S-transferase (GST) fusion protein comprising GST and the PH domain of PLD1 (GST-PLD1-PH) also bound specifically to supported lipid monolayers containing PI(4,5)P2.	pi(4,5)p2	182-191	phospholipase D1	Homo sapiens	104-115
10196179-9	1999	In contrast, the E41K mutation, which confers transforming activity to native Btk, caused significant membrane localization of BtkPH-GFP with characteristics indicating its possible binding to PI(4,5)P2.	pi(4,5)p2	193-202	Bruton agammaglobulinemia tyrosine kinase	Mus musculus	78-81
10085097-6	1999	In contrast, PI-PLDc preferentially utilizes PI(3)P as substrate, followed by, in sequence, PI(3,4,5)P3, PI(4)P, PI(3,4)P2, and PI(4,5)P2.	pi(4,5)p2	128-137	phospholipase D2	Rattus norvegicus	16-20
8999863-3	1997	A glutathione S-transferase fusion protein containing the pleckstrin homology (PH) domain of mSos1 bound specifically and tightly to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) with a Kd of 1.8 +/- 0.4 microM.	pi(4,5)p2	172-181	SOS Ras/Rac guanine nucleotide exchange factor 1	Mus musculus	93-98
9550703-3	1998	Members of this family contain a carboxy-terminal pleckstrin homology (PH) domain which, in the case of GRP-1, has been shown to bind the second messenger phosphatidylinositol 3,4,5-trisphosphate (PIP3) in preference to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and phosphatidylinositol 3,4-bisphosphate (PI(3,4)P2) in vitro [3,4].	pi(4,5)p2	259-268	glutamine repeat protein 1	Mus musculus	104-109
9195925-12	1997	However, E54K shows a dramatically increased rate of (PI(4, 5)P2)-stimulated PI(4,5)P2 hydrolysis (interfacial Vmax for PLC delta1 = 4.9 +/- 0.3 micromol/min/mg and for E54K = 31 +/- 3 micromol/min/mg) as well as PI hydrolysis (Vmax for PLC delta1 = 27 +/- 3.4 nmol/min/mg and for E54K = 95 +/- 12 nmol/min/mg).	pi(4,5)p2	77-86	phospholipase C delta 1	Homo sapiens	120-130
9195925-12	1997	However, E54K shows a dramatically increased rate of (PI(4, 5)P2)-stimulated PI(4,5)P2 hydrolysis (interfacial Vmax for PLC delta1 = 4.9 +/- 0.3 micromol/min/mg and for E54K = 31 +/- 3 micromol/min/mg) as well as PI hydrolysis (Vmax for PLC delta1 = 27 +/- 3.4 nmol/min/mg and for E54K = 95 +/- 12 nmol/min/mg).	pi(4,5)p2	77-86	phospholipase C delta 1	Homo sapiens	237-247
9188725-8	1997	The data are consistent with the idea that the PH domain of PLC-delta1, but not the beta isozymes, directs the catalytic core to membranes enriched in PI(4,5)P2 and is subject to product inhibition.	pi(4,5)p2	151-160	phospholipase C delta 1	Homo sapiens	60-70
8999863-5	1997	Substitution of Arg452 within the PH domain with Ala had only a slight effect on binding to PI(4,5)P2, whereas substitution of Arg459 severely compromised the ability of the mSos1 PH domain to bind to PI(4,5)P2 containing vesicles.	pi(4,5)p2	201-210	SOS Ras/Rac guanine nucleotide exchange factor 1	Mus musculus	174-179
8999863-6	1997	Purified full-length mSos1 and mSos1 complexed with Grb2 were also tested for binding to various phosphoinositol derivatives and demonstrated a specific interaction with PI(4,5)P2, although these interactions were weaker (Kd = approximately 53 and approximately 69 microM, respectively) than that of the PH domain alone.	pi(4,5)p2	170-179	SOS Ras/Rac guanine nucleotide exchange factor 1	Mus musculus	21-26
8999863-6	1997	Purified full-length mSos1 and mSos1 complexed with Grb2 were also tested for binding to various phosphoinositol derivatives and demonstrated a specific interaction with PI(4,5)P2, although these interactions were weaker (Kd = approximately 53 and approximately 69 microM, respectively) than that of the PH domain alone.	pi(4,5)p2	170-179	SOS Ras/Rac guanine nucleotide exchange factor 1	Mus musculus	31-36
8999863-6	1997	Purified full-length mSos1 and mSos1 complexed with Grb2 were also tested for binding to various phosphoinositol derivatives and demonstrated a specific interaction with PI(4,5)P2, although these interactions were weaker (Kd = approximately 53 and approximately 69 microM, respectively) than that of the PH domain alone.	pi(4,5)p2	170-179	growth factor receptor bound protein 2	Homo sapiens	52-56
8999863-7	1997	These findings suggest that the PH domain of mSos1 can interact in vitro with phospholipid vesicles containing PI(4,5)P2 and that this interaction is facilitated by the ionic interaction of Arg459 with the negatively charged head group of PI(4,5)P2.	pi(4,5)p2	111-120	SOS Ras/Rac guanine nucleotide exchange factor 1	Mus musculus	45-50
8394081-7	1993	Thrombin stimulation of genistein-pretreated cells intensified this tendency, i.e. a further increase in the amount of PI(4)P and a decrease in the amount of PI(4,5)P2 in an inversely proportional manner.	pi(4,5)p2	158-167	coagulation factor II, thrombin	Homo sapiens	0-8
8942652-1	1996	We measured the ability of sphingomyelin (SPM) to inhibit phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] hydrolysis catalyzed by human phospholipase C-delta 1 (PLC-delta 1) in model membranes and detergent phospholipid mixed micelles.	pi(4,5)p2	97-106	phospholipase C delta 1	Homo sapiens	138-161
8810277-6	1996	Pleckstrin-mediated inhibition of PI3Kgamma is overcome by excess Gbetagamma and is restricted to PI(4,5)P2 as substrate, i.e. pleckstrin does not inhibit phosphorylation of PI()P or PI.	pi(4,5)p2	98-107	pleckstrin	Homo sapiens	0-10
8810277-7	1996	Consistent with this, activation of protein kinase C by exposure of platelets to beta-phorbol diester (to increase endogenous pleckstrin phosphorylation) prior to platelet lysis causes inhibition of Gbetagamma-stimulatable PI3K activity only with respect to PI(4,5)P2 substrate.	pi(4,5)p2	258-267	pleckstrin	Homo sapiens	126-136
1320642-5	1992	When neutrophils were preincubated with C5a for 5 min before stimulation with formyl peptide, [32P]PI(4,5)P2 hydrolysis was unchanged, and [32P]PA production and O2- formation were slightly enhanced compared with controls stimulated with formyl peptide in the absence of C5a.	pi(4,5)p2	99-108	complement C5a receptor 1	Homo sapiens	40-43
34864549-5	2022	Intriguingly, liposome binding assays using various phospholipids and PA species revealed that AP180 most strongly bound to 1-stearoyl-2-docosahexaenoyl-PA (18:0/22:6-PA) to a comparable extent as phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), which is known to associate with AP180.	pi(4,5)p2	236-245	synaptosomal-associated protein 91	Mus musculus	281-286
34569608-5	2022	Spacing of palmitoyl groups within three doubly palmitoylated EFR3B "lipoforms" affects both its interactions with TMEM150A, a transmembrane protein governing formation of a PI4KIIIalpha complex functioning in rapid PI(4,5)P2 resynthesis following PLC signaling, and its partitioning within liquid-ordered and -disordered regions of the plasma membrane.	pi(4,5)p2	216-225	EFR3 homolog B	Homo sapiens	62-67
34569608-5	2022	Spacing of palmitoyl groups within three doubly palmitoylated EFR3B "lipoforms" affects both its interactions with TMEM150A, a transmembrane protein governing formation of a PI4KIIIalpha complex functioning in rapid PI(4,5)P2 resynthesis following PLC signaling, and its partitioning within liquid-ordered and -disordered regions of the plasma membrane.	pi(4,5)p2	216-225	transmembrane protein 150A	Homo sapiens	115-123
34864207-6	2022	ORP2, the closest relative of ORP1, is mainly cytosolic, but also targets PI(4,5)P2-rich endosomal compartments.	pi(4,5)p2	74-83	oxysterol binding protein like 2	Homo sapiens	0-4
34864207-6	2022	ORP2, the closest relative of ORP1, is mainly cytosolic, but also targets PI(4,5)P2-rich endosomal compartments.	pi(4,5)p2	74-83	oxysterol binding protein like 1A	Homo sapiens	30-34
34864207-7	2022	Our latest data suggest that ORP2 transfers cholesterol from LE to recycling endosomes (RE) in exchange for PI(4,5)P2, thus stimulating the recruitment of focal adhesion kinase (FAK) on the RE and cell adhesion.	pi(4,5)p2	108-117	oxysterol binding protein like 2	Homo sapiens	29-33
34864207-7	2022	Our latest data suggest that ORP2 transfers cholesterol from LE to recycling endosomes (RE) in exchange for PI(4,5)P2, thus stimulating the recruitment of focal adhesion kinase (FAK) on the RE and cell adhesion.	pi(4,5)p2	108-117	protein tyrosine kinase 2	Homo sapiens	155-176
34864207-7	2022	Our latest data suggest that ORP2 transfers cholesterol from LE to recycling endosomes (RE) in exchange for PI(4,5)P2, thus stimulating the recruitment of focal adhesion kinase (FAK) on the RE and cell adhesion.	pi(4,5)p2	108-117	protein tyrosine kinase 2	Homo sapiens	178-181
34864207-8	2022	FAK activates phosphoinositide kinase on the RE to enhance PI(4,5)P2 synthesis.	pi(4,5)p2	59-68	protein tyrosine kinase 2	Homo sapiens	0-3
34864207-9	2022	ORP2 in turn transfers PI(4,5)P2 from RE to LE, thus regulating LE tubule formation and transport activity.	pi(4,5)p2	23-32	oxysterol binding protein like 2	Homo sapiens	0-4
34883117-1	2022	Plasma-membrane-specific localization of Gag, an essential step in HIV-1 particle assembly, is regulated by the interaction of the Gag MA domain with PI(4,5)P2 and tRNA-mediated inhibition of non-specific or premature membrane binding.	pi(4,5)p2	150-159	Pr55(Gag)	Human immunodeficiency virus 1	41-44
34883117-1	2022	Plasma-membrane-specific localization of Gag, an essential step in HIV-1 particle assembly, is regulated by the interaction of the Gag MA domain with PI(4,5)P2 and tRNA-mediated inhibition of non-specific or premature membrane binding.	pi(4,5)p2	150-159	Pr55(Gag)	Human immunodeficiency virus 1	131-134
34922306-1	2022	Phosphatidyl inositol (4,5)-bisphosphate (PI(4,5)P2) plays several key roles in human biology and the lipid kinase that produces PI(4,5)P2, PIP5K, has been hypothesized to provide a potential therapeutic target of interest in the treatment of cancers.	pi(4,5)p2	42-51	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	140-145
34922306-1	2022	Phosphatidyl inositol (4,5)-bisphosphate (PI(4,5)P2) plays several key roles in human biology and the lipid kinase that produces PI(4,5)P2, PIP5K, has been hypothesized to provide a potential therapeutic target of interest in the treatment of cancers.	pi(4,5)p2	129-138	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	140-145
33771931-3	2022	PI(4,5)P2 normally restricted to the proximal segment redistributed to the entire length of cilia in Inpp5e knockout mice with a reduction in PI(3,4)P2 and elevation of PI(3,4,5)P3 in the dendritic knob.	pi(4,5)p2	0-9	inositol polyphosphate-5-phosphatase E	Mus musculus	101-107
34821358-3	2022	The recently characterized lipid transfer protein TMEM24 dynamically localize to ER-PM contact sites and provide phosphatidylinositol, a precursor of PI(4)P and PI(4,5)P2, to the plasma membrane.	pi(4,5)p2	161-170	C2CD2 like	Homo sapiens	50-56
34947862-8	2021	We show that optimized protocol allows for the visualization of PI(4,5)P2 and PI4P at PM in activated PLTs, which could also be modulated by OCRL and PI4KIIIalpha inhibitors.	pi(4,5)p2	64-73	OCRL inositol polyphosphate-5-phosphatase	Homo sapiens	141-145
34846128-2	2021	Previous studies have established that phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is required for Kir3.4 function.	pi(4,5)p2	78-87	potassium inwardly rectifying channel subfamily J member 5	Homo sapiens	105-111
34846128-7	2021	The binding of PI(4,5)P2 modified with a fluorophore to Kir3.2 can be enhanced by other lipids, such as phosphatidylcholine.	pi(4,5)p2	15-24	potassium inwardly rectifying channel subfamily J member 6	Homo sapiens	56-62
34846128-9	2021	In contrast, the D223N mutant of Kir3.4 that mimics the sodium-bound state exhibited stronger binding for PI(4,5)P2, particularly for those with 18:0-20:4 acyl chains.	pi(4,5)p2	106-115	potassium inwardly rectifying channel subfamily J member 5	Homo sapiens	33-39
34855407-2	2022	Colloidal probe force spectroscopy in conjunction with tethered particle motion monitoring showed that in the absence of Ca2+ the binding of syt-1 to membranes depends on the presence and content of PI(4,5)P2.	pi(4,5)p2	199-208	synaptotagmin 1	Homo sapiens	141-146
34855407-4	2022	Fusion of single unilamellar vesicles equipped with syt-1 and synaptobrevin 2 with planar pore-spanning target membranes containing PS and PI(4,5)P2 shows an almost complete suppression of stalled intermediate fusion states and an accelerated fusion kinetics in the presence of Ca2+, which is further enhanced upon addition of ATP.	pi(4,5)p2	139-148	synaptotagmin 1	Homo sapiens	52-57
34855407-4	2022	Fusion of single unilamellar vesicles equipped with syt-1 and synaptobrevin 2 with planar pore-spanning target membranes containing PS and PI(4,5)P2 shows an almost complete suppression of stalled intermediate fusion states and an accelerated fusion kinetics in the presence of Ca2+, which is further enhanced upon addition of ATP.	pi(4,5)p2	139-148	vesicle associated membrane protein 2	Homo sapiens	62-77
34478732-0	2021	TXNIP interaction with GLUT1 depends on PI(4,5)P2.	pi(4,5)p2	40-49	thioredoxin interacting protein	Homo sapiens	0-5
34478732-0	2021	TXNIP interaction with GLUT1 depends on PI(4,5)P2.	pi(4,5)p2	40-49	solute carrier family 2 member 1	Homo sapiens	23-28
34478732-6	2021	We found that GLUT1 lipid nanodiscs and TXNIP interact in a 1:1 ratio and that this interaction requires phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 or PIP2).	pi(4,5)p2	144-153	solute carrier family 2 member 1	Homo sapiens	14-19
34478732-6	2021	We found that GLUT1 lipid nanodiscs and TXNIP interact in a 1:1 ratio and that this interaction requires phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 or PIP2).	pi(4,5)p2	144-153	thioredoxin interacting protein	Homo sapiens	40-45
34947862-6	2021	Here, we show that the intracellular pools of PI(4,5)P2 and PI4P can be detected by the established staining protocol, and these pools can be modulated by inhibitors of OCRL phosphatase and PI4KIIIalpha kinase.	pi(4,5)p2	46-55	OCRL inositol polyphosphate-5-phosphatase	Homo sapiens	169-173
34563650-6	2021	In hepatocytes, alpha-TTP catalyzes the vectorial transport of alpha-Toc from the endocytotic compartment to the plasma membrane (PM) by targeting phosphatidylinositol phosphates (PIPs) such as PI(4,5)P2.	pi(4,5)p2	194-203	alpha tocopherol transfer protein	Homo sapiens	16-25
34758297-0	2021	PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane.	pi(4,5)p2	19-28	peripheral myelin protein 2	Homo sapiens	0-4
34758297-0	2021	PMP2/FABP8 induces PI(4,5)P2-dependent transbilayer reorganization of sphingomyelin in the plasma membrane.	pi(4,5)p2	19-28	peripheral myelin protein 2	Homo sapiens	5-10
34758297-4	2021	A biochemical assay demonstrates that PMP2 is a phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2)-binding protein.	pi(4,5)p2	87-96	peripheral myelin protein 2	Homo sapiens	38-42
34758297-5	2021	PMP2 induces the tubulation of model membranes in a PI(4,5)P2-dependent manner, accompanied by the modification of the transbilayer membrane distribution of lipids.	pi(4,5)p2	52-61	peripheral myelin protein 2	Homo sapiens	0-4
34758297-8	2021	A mutation in PMP2 associated with CMT increases its affinity for PI(4,5)P2, inducing membrane tubulation and the subsequent transbilayer movement of lipids.	pi(4,5)p2	66-75	peripheral myelin protein 2	Homo sapiens	14-18
34655614-3	2021	Here we show that Smad2 selectively and tightly binds phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) in the PM.	pi(4,5)p2	93-102	SMAD family member 2	Homo sapiens	18-23
34655614-4	2021	The PI(4,5)P2-binding site is located in the MH2 domain that is involved in interaction with the TGF-beta receptor I that transduces TGF-beta-receptor binding to downstream signaling proteins.	pi(4,5)p2	4-13	transforming growth factor alpha	Homo sapiens	133-141
34655614-5	2021	Quantitative optical imaging analyses show that PM recruitment of Smad2 is triggered by its interaction with PI(4,5)P2 that is locally enriched near the activated TGF-beta receptor complex, leading to its binding to the TGF-beta receptor I.	pi(4,5)p2	109-118	SMAD family member 2	Homo sapiens	66-71
34655614-5	2021	Quantitative optical imaging analyses show that PM recruitment of Smad2 is triggered by its interaction with PI(4,5)P2 that is locally enriched near the activated TGF-beta receptor complex, leading to its binding to the TGF-beta receptor I.	pi(4,5)p2	109-118	transforming growth factor alpha	Homo sapiens	163-171
34655614-6	2021	The PI(4,5)P2 binding activity of Smad2 is essential for the TGF-beta-stimulated phosphorylation, nuclear transport and transcriptional activity of Smad2.	pi(4,5)p2	4-13	SMAD family member 2	Homo sapiens	34-39
34655614-6	2021	The PI(4,5)P2 binding activity of Smad2 is essential for the TGF-beta-stimulated phosphorylation, nuclear transport and transcriptional activity of Smad2.	pi(4,5)p2	4-13	transforming growth factor alpha	Homo sapiens	61-69
34655614-6	2021	The PI(4,5)P2 binding activity of Smad2 is essential for the TGF-beta-stimulated phosphorylation, nuclear transport and transcriptional activity of Smad2.	pi(4,5)p2	4-13	SMAD family member 2	Homo sapiens	148-153
34827112-8	2021	We showed that PI(4,5)P2-dependent TREK-2 activity occurs when the conditions for PI(4,5)P2/Ca2+ nanocluster formation are met.	pi(4,5)p2	15-24	potassium two pore domain channel subfamily K member 10	Rattus norvegicus	35-41
34827112-8	2021	We showed that PI(4,5)P2-dependent TREK-2 activity occurs when the conditions for PI(4,5)P2/Ca2+ nanocluster formation are met.	pi(4,5)p2	82-91	potassium two pore domain channel subfamily K member 10	Rattus norvegicus	35-41
34663803-4	2021	Using the C. elegans early embryo as our model system, we show that the evolutionarily conserved lipid transfer proteins, PDZD-8 and TEX-2, act together with the PI(4,5)P2 phosphatases, OCRL-1 and UNC-26/synaptojanin, to prevent the build-up of PI(4,5)P2 on endosomal membranes.	pi(4,5)p2	245-254	Rho-GAP domain-containing protein	Caenorhabditis elegans	186-192
34663803-4	2021	Using the C. elegans early embryo as our model system, we show that the evolutionarily conserved lipid transfer proteins, PDZD-8 and TEX-2, act together with the PI(4,5)P2 phosphatases, OCRL-1 and UNC-26/synaptojanin, to prevent the build-up of PI(4,5)P2 on endosomal membranes.	pi(4,5)p2	245-254	Synaptojanin	Caenorhabditis elegans	197-203
34273353-0	2021	Binding to PI(4,5)P2 is indispensable for secretion of B cell clonogenic HIV-1 matrix protein p17 variants.	pi(4,5)p2	11-20	family with sequence similarity 72 member B	Homo sapiens	94-97
34298060-2	2021	For many retroviruses, Gag-PM interaction is dependent on phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	97-106	Pr55	Human T-cell leukemia virus type I	23-26
34298060-3	2021	However, it has been shown that for human T-cell leukemia virus type 1 (HTLV-1), Gag binding to membranes is less dependent on PI(4,5)P2 than HIV-1, suggesting that other factors may modulate Gag assembly.	pi(4,5)p2	127-136	Pr55	Human T-cell leukemia virus type I	81-84
34298060-3	2021	However, it has been shown that for human T-cell leukemia virus type 1 (HTLV-1), Gag binding to membranes is less dependent on PI(4,5)P2 than HIV-1, suggesting that other factors may modulate Gag assembly.	pi(4,5)p2	127-136	Pr55(Gag)	Human immunodeficiency virus 1	192-195
34541033-4	2021	In CDCP1low DU145 and PC3 prostate cancer cells, detachment-activation of FAK occurs through local production of PI(4,5)P2.	pi(4,5)p2	113-122	protein tyrosine kinase 2	Homo sapiens	74-77
34541033-5	2021	PI(4,5)P2 is generated by the PIP5K1c-201 splicing isoform of PIP5K1c, which contains a unique SRC phosphorylation site.	pi(4,5)p2	0-9	phosphatidylinositol-4-phosphate 5-kinase type 1 gamma	Homo sapiens	30-37
34541033-5	2021	PI(4,5)P2 is generated by the PIP5K1c-201 splicing isoform of PIP5K1c, which contains a unique SRC phosphorylation site.	pi(4,5)p2	0-9	phosphatidylinositol-4-phosphate 5-kinase type 1 gamma	Homo sapiens	62-69
34541033-5	2021	PI(4,5)P2 is generated by the PIP5K1c-201 splicing isoform of PIP5K1c, which contains a unique SRC phosphorylation site.	pi(4,5)p2	0-9	SRC proto-oncogene, non-receptor tyrosine kinase	Homo sapiens	95-98
34624591-1	2021	Phosphatidylinositol(4,5)bisphosphate (PI(4,5)P2) produced by phosphatidylinositol phosphate 5 kinase (PIP5K) plays not only as a precursor of second messengers in the phosphoinositide signal transduction, but also multiple roles influencing a variety of cellular activities.	pi(4,5)p2	39-48	phosphoinositide kinase, FYVE type zinc finger containing	Mus musculus	62-101
34624591-1	2021	Phosphatidylinositol(4,5)bisphosphate (PI(4,5)P2) produced by phosphatidylinositol phosphate 5 kinase (PIP5K) plays not only as a precursor of second messengers in the phosphoinositide signal transduction, but also multiple roles influencing a variety of cellular activities.	pi(4,5)p2	39-48	phosphoinositide kinase, FYVE type zinc finger containing	Mus musculus	103-108
34624591-6	2021	The present study provides evidence suggesting that PI(4,5)P2 locally synthesized by PIP5K in LDs is involved in the lipid transfer between lipid droplets (LDs) and the endoplasmic reticulum, which eventually regulates ovarian progesterone production through control of multiple dynamic activities of LDs.	pi(4,5)p2	52-61	phosphoinositide kinase, FYVE type zinc finger containing	Mus musculus	85-90
34273353-6	2021	Binding of Pr55Gag to PI(4,5)P2 was indispensable for allowing the unconventional secretion of both wild-type p17 and vp17s.	pi(4,5)p2	22-31	family with sequence similarity 72 member B	Homo sapiens	110-113
34124795-0	2021	ORP2 couples LDL-cholesterol transport to FAK activation by endosomal cholesterol/PI(4,5)P2 exchange.	pi(4,5)p2	82-91	oxysterol binding protein like 2	Homo sapiens	0-4
34267198-4	2021	Further investigation of ARHGEF3 reveals distinct structural requirements for its binding to PI(4,5)P2 and PI(3,5)P2, and functional relevance of its PI(4,5)P2 binding.	pi(4,5)p2	93-102	Rho guanine nucleotide exchange factor 3	Homo sapiens	25-32
34267198-4	2021	Further investigation of ARHGEF3 reveals distinct structural requirements for its binding to PI(4,5)P2 and PI(3,5)P2, and functional relevance of its PI(4,5)P2 binding.	pi(4,5)p2	150-159	Rho guanine nucleotide exchange factor 3	Homo sapiens	25-32
34239035-5	2021	We found that betaA3/A1-crystallin binds to phosphatidylinositol transfer protein (PITPbeta) and that betaA3/A1-crystallin deficiency diminishes phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), thus probably decreasing ezrin phosphorylation, EGFR activation, internalization, and degradation.	pi(4,5)p2	183-192	ezrin	Homo sapiens	220-225
34239035-5	2021	We found that betaA3/A1-crystallin binds to phosphatidylinositol transfer protein (PITPbeta) and that betaA3/A1-crystallin deficiency diminishes phosphatidylinositol 4,5-biphosphate (PI(4,5)P2), thus probably decreasing ezrin phosphorylation, EGFR activation, internalization, and degradation.	pi(4,5)p2	183-192	epidermal growth factor receptor	Homo sapiens	243-247
34239035-6	2021	We propose that betaA3/A1-crystallin acquired its RPE function before evolving as a structural element in the lens, and that in the RPE, it modulates the PI(4,5)P2 pool through PITPbeta/PLC signaling axis, coordinates EGFR activation, regulates ezrin phosphorylation and ultimately the cell polarity.	pi(4,5)p2	154-163	phosphatidylinositol transfer protein beta	Homo sapiens	177-185
34111256-3	2021	Recently, we used an optogenetic approach in TM to regulate the 5-phosphatase, OCRL, which contributes to regulating PI(4,5)P2 levels.	pi(4,5)p2	117-126	OCRL, inositol polyphosphate-5-phosphatase	Mus musculus	79-83
34124795-0	2021	ORP2 couples LDL-cholesterol transport to FAK activation by endosomal cholesterol/PI(4,5)P2 exchange.	pi(4,5)p2	82-91	protein tyrosine kinase 2	Homo sapiens	42-45
34124795-3	2021	In vitro, cholesterol enhances membrane association of FAK to PI(4,5)P2 -containing lipid bilayers.	pi(4,5)p2	62-71	protein tyrosine kinase 2	Homo sapiens	55-58
34124795-4	2021	In cells, ORP2 stimulates FAK activation and PI(4,5)P2 generation in endomembranes, enhancing cell adhesion.	pi(4,5)p2	45-54	oxysterol binding protein like 2	Homo sapiens	10-14
34124795-5	2021	Moreover, ORP2 increases PI(4,5)P2 in NPC1-containing late endosomes in a FAK-dependent manner, controlling their tubulovesicular trafficking.	pi(4,5)p2	25-34	oxysterol binding protein like 2	Homo sapiens	10-14
34124795-5	2021	Moreover, ORP2 increases PI(4,5)P2 in NPC1-containing late endosomes in a FAK-dependent manner, controlling their tubulovesicular trafficking.	pi(4,5)p2	25-34	NPC intracellular cholesterol transporter 1	Homo sapiens	38-42
34124795-5	2021	Moreover, ORP2 increases PI(4,5)P2 in NPC1-containing late endosomes in a FAK-dependent manner, controlling their tubulovesicular trafficking.	pi(4,5)p2	25-34	protein tyrosine kinase 2	Homo sapiens	74-77
34124795-6	2021	Together, these results provide evidence that ORP2 controls FAK activation and LDL-cholesterol plasma membrane delivery by promoting bidirectional cholesterol/PI(4,5)P2 exchange between late and recycling endosomes.	pi(4,5)p2	159-168	oxysterol binding protein like 2	Homo sapiens	46-50
34124795-6	2021	Together, these results provide evidence that ORP2 controls FAK activation and LDL-cholesterol plasma membrane delivery by promoting bidirectional cholesterol/PI(4,5)P2 exchange between late and recycling endosomes.	pi(4,5)p2	159-168	protein tyrosine kinase 2	Homo sapiens	60-63
35490898-3	2022	For many retroviruses, Gag-PM interactions are mediated by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2); however, previous studies suggested that HTLV-1 Gag-PM interactions and therefore virus assembly are less dependent on PI(4,5)P2.	pi(4,5)p2	98-107	Pr55	Human T-cell leukemia virus type I	23-26
35490898-3	2022	For many retroviruses, Gag-PM interactions are mediated by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2); however, previous studies suggested that HTLV-1 Gag-PM interactions and therefore virus assembly are less dependent on PI(4,5)P2.	pi(4,5)p2	98-107	Pr55	Human T-cell leukemia virus type I	158-161
35490898-3	2022	For many retroviruses, Gag-PM interactions are mediated by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2); however, previous studies suggested that HTLV-1 Gag-PM interactions and therefore virus assembly are less dependent on PI(4,5)P2.	pi(4,5)p2	229-238	Pr55	Human T-cell leukemia virus type I	23-26
35490898-3	2022	For many retroviruses, Gag-PM interactions are mediated by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2); however, previous studies suggested that HTLV-1 Gag-PM interactions and therefore virus assembly are less dependent on PI(4,5)P2.	pi(4,5)p2	229-238	Pr55	Human T-cell leukemia virus type I	158-161
35451158-6	2022	The lipid transfer activity of SYT1 is highly dependent on PI(4,5)P2 , a signal lipid accumulated at the PM under abiotic stress.	pi(4,5)p2	59-68	synaptotagmin A	Arabidopsis thaliana	31-35
35609603-6	2022	Through reconstitution and epithelial cell biology experiments, we show that Arf6-mediated recruitment of the lipid kinase PIP5K1C rapidly converts phosphatidylinositol 4-phosphate (PI(4)P) to PI(4,5)P2, driving exocyst recruitment and membrane tethering.	pi(4,5)p2	193-202	ADP ribosylation factor 6	Homo sapiens	77-81
35521907-6	2022	The X and Y catalytic domains are responsible for the catalysis of the phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) substrate to produce the Ca2+-mobilising messenger, inositol 1,4,5-trisphosphate (IP3), while the XY linker plays multiple roles in the unique mode of PLCzeta action.	pi(4,5)p2	110-119	phospholipase C zeta 1	Homo sapiens	272-279
35605664-5	2022	Here we used fluorescence-based assays to demonstrate a phosphatidylinositol phosphate (PIP)-selective mechanism by which StarD4 can preferentially extract sterol from liposome membranes containing certain PIPs (especially, PI(4,5)P2 and to a lesser degree PI(3,5)P2).	pi(4,5)p2	224-233	StAR related lipid transfer domain containing 4	Homo sapiens	122-128
35609603-6	2022	Through reconstitution and epithelial cell biology experiments, we show that Arf6-mediated recruitment of the lipid kinase PIP5K1C rapidly converts phosphatidylinositol 4-phosphate (PI(4)P) to PI(4,5)P2, driving exocyst recruitment and membrane tethering.	pi(4,5)p2	193-202	phosphatidylinositol-4-phosphate 5-kinase type 1 gamma	Homo sapiens	123-130
35534464-6	2022	PM localization of PARD3 is impaired by depletion of PM PI(4,5)P2 in epithelial cells, whereas expression of the PM-targeting exocyst-docking region of PARD3 induces osteosarcoma cells to show epithelial-like morphological changes, suggesting that PI(4,5)P2 regulates epithelial characteristics by recruiting PARD3 to the PM.	pi(4,5)p2	248-257	par-3 family cell polarity regulator	Homo sapiens	152-157
35568951-1	2022	BACKGROUND: Inositol Polyphosphate-5-Phosphatase B (INPP5B), a inositol 5-phosphatase, plays an important role in many biological processes through phosphorylating PI(4,5)P2 and/or PI(3,4,5)P3 at the 5-position.	pi(4,5)p2	164-173	inositol polyphosphate-5-phosphatase B	Homo sapiens	12-50
35568951-1	2022	BACKGROUND: Inositol Polyphosphate-5-Phosphatase B (INPP5B), a inositol 5-phosphatase, plays an important role in many biological processes through phosphorylating PI(4,5)P2 and/or PI(3,4,5)P3 at the 5-position.	pi(4,5)p2	164-173	inositol polyphosphate-5-phosphatase B	Homo sapiens	52-58
35416932-3	2022	Here, we report that PI(4,5)P2 regulates TRPC3 in three independent modes.	pi(4,5)p2	21-30	transient receptor potential cation channel subfamily C member 3	Homo sapiens	41-46
35416932-5	2022	PI(4,5)P2 interacts with lipid site 1 to inhibit TRPC3 opening and regulate access of DAG to the pore lipid site 2.	pi(4,5)p2	0-9	transient receptor potential cation channel subfamily C member 3	Homo sapiens	49-54
35416932-7	2022	Notably, the activation and regulation of TRPC3 by PI(4,5)P2 require recruitment of TRPC3 to the ER/PM junctions at a PI(4,5)P2-rich domain.	pi(4,5)p2	51-60	transient receptor potential cation channel subfamily C member 3	Homo sapiens	42-47
35416932-7	2022	Notably, the activation and regulation of TRPC3 by PI(4,5)P2 require recruitment of TRPC3 to the ER/PM junctions at a PI(4,5)P2-rich domain.	pi(4,5)p2	51-60	transient receptor potential cation channel subfamily C member 3	Homo sapiens	84-89
35277468-0	2022	Septins tune lipid kinase activity and PI(4,5)P2 turnover during G-protein-coupled PLC signalling in vivo.	pi(4,5)p2	39-48	Phospholipase C at 21C	Drosophila melanogaster	83-86
35476976-2	2022	The basal activity of TRPV5 is balanced through activation by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and inhibition by Ca2+-bound calmodulin (CaM).	pi(4,5)p2	101-110	transient receptor potential cation channel subfamily V member 5	Homo sapiens	22-27
35476976-5	2022	Using cryoelectron microscopy (cryo-EM), we show that low pH inhibits TRPV5 by precluding PI(4,5)P2 activation.	pi(4,5)p2	90-99	transient receptor potential cation channel subfamily V member 5	Homo sapiens	70-75
35476976-7	2022	In addition, we demonstrate that PI(4,5)P2 is the primary modulator of channel gating, yet PKA controls TRPV5 activity by preventing CaM binding and channel inactivation.	pi(4,5)p2	33-42	transient receptor potential cation channel subfamily V member 5	Homo sapiens	104-109
35437696-5	2022	Knockdown of Skittles, a phosphatidylinositol(4)phosphate 5-kinase, which produces PI(4,5)P2, abolished slit diaphragm formation and led to strongly reduced endocytosis.	pi(4,5)p2	83-92	skittles	Drosophila melanogaster	25-66
35319770-7	2022	We further show that the mTORC2-Rab35 axis plays an essential role in the defense against hypotonic stress by promoting the degradation of the actin cortex through the up-regulation of PI(4,5)P2 metabolism, which facilitates the apical tethering of sphingomyelin-loaded vesicles to relieve plasma membrane tension.	pi(4,5)p2	185-194	CREB regulated transcription coactivator 2	Mus musculus	25-31
35319770-7	2022	We further show that the mTORC2-Rab35 axis plays an essential role in the defense against hypotonic stress by promoting the degradation of the actin cortex through the up-regulation of PI(4,5)P2 metabolism, which facilitates the apical tethering of sphingomyelin-loaded vesicles to relieve plasma membrane tension.	pi(4,5)p2	185-194	RAB35, member RAS oncogene family	Homo sapiens	32-37
35277468-1	2022	Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) hydrolysis by phospholipase C (PLC) is a conserved mechanism of signalling.	pi(4,5)p2	39-48	Phospholipase C at 21C	Drosophila melanogaster	64-79
35277468-1	2022	Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) hydrolysis by phospholipase C (PLC) is a conserved mechanism of signalling.	pi(4,5)p2	39-48	Phospholipase C at 21C	Drosophila melanogaster	81-84
35277468-2	2022	Given the low abundance of PI(4,5)P2, its hydrolysis needs to be coupled to resynthesis to ensure continued PLC activity; however, the mechanism by which depletion is coupled to resynthesis remains unknown.	pi(4,5)p2	27-36	Phospholipase C at 21C	Drosophila melanogaster	108-111
35277468-3	2022	PI(4,5)P2 synthesis is catalyzed by the phosphorylation of phosphatidylinositol 4 phosphate (PI4P) by phosphatidylinositol 4 phosphate 5 kinase (PIP5K).	pi(4,5)p2	0-9	skittles	Drosophila melanogaster	102-143
35277468-3	2022	PI(4,5)P2 synthesis is catalyzed by the phosphorylation of phosphatidylinositol 4 phosphate (PI4P) by phosphatidylinositol 4 phosphate 5 kinase (PIP5K).	pi(4,5)p2	0-9	skittles	Drosophila melanogaster	145-150
35277468-4	2022	In Drosophila photoreceptors, photon absorption is transduced into PLC activity and during this process, PI(4,5)P2 is resynthesized by a PIP5K.	pi(4,5)p2	105-114	Phospholipase C at 21C	Drosophila melanogaster	67-70
35277468-4	2022	In Drosophila photoreceptors, photon absorption is transduced into PLC activity and during this process, PI(4,5)P2 is resynthesized by a PIP5K.	pi(4,5)p2	105-114	skittles	Drosophila melanogaster	137-142
35277468-5	2022	However, the mechanism by which PIP5K activity is coupled to PI(4,5)P2 hydrolysis is unknown.	pi(4,5)p2	61-70	skittles	Drosophila melanogaster	32-37
35277468-7	2022	Depletion of PNUT, a non-redundant subunit of the septin family, enhances dPIP5KL activity in vitro and PI(4,5)P2 resynthesis in vivo; co-depletion of dPIP5KL reverses the enhanced rate of PI(4,5)P2 resynthesis in vivo.	pi(4,5)p2	104-113	peanut	Drosophila melanogaster	13-17
35277468-7	2022	Depletion of PNUT, a non-redundant subunit of the septin family, enhances dPIP5KL activity in vitro and PI(4,5)P2 resynthesis in vivo; co-depletion of dPIP5KL reverses the enhanced rate of PI(4,5)P2 resynthesis in vivo.	pi(4,5)p2	189-198	peanut	Drosophila melanogaster	13-17
35277468-8	2022	Thus, our work defines a septin-mediated mechanism through which PIP5K activity is coupled to PLC-mediated PI(4,5)P2 hydrolysis.	pi(4,5)p2	107-116	Septin 1	Drosophila melanogaster	25-31
35277468-8	2022	Thus, our work defines a septin-mediated mechanism through which PIP5K activity is coupled to PLC-mediated PI(4,5)P2 hydrolysis.	pi(4,5)p2	107-116	skittles	Drosophila melanogaster	65-70
35277468-8	2022	Thus, our work defines a septin-mediated mechanism through which PIP5K activity is coupled to PLC-mediated PI(4,5)P2 hydrolysis.	pi(4,5)p2	107-116	Phospholipase C at 21C	Drosophila melanogaster	94-97
35311809-2	2022	PLCbeta activity results in the hydrolysis of the membrane lipid phosphatidylinositol 4,5 bisphosphate (PI(4,5)P2) leading ultimately to the activation of transient receptor potential (TRP) and TRP like (TRPL) channels.	pi(4,5)p2	104-113	no receptor potential A	Drosophila melanogaster	0-7
35311809-2	2022	PLCbeta activity results in the hydrolysis of the membrane lipid phosphatidylinositol 4,5 bisphosphate (PI(4,5)P2) leading ultimately to the activation of transient receptor potential (TRP) and TRP like (TRPL) channels.	pi(4,5)p2	104-113	transient receptor potential-like	Drosophila melanogaster	194-202
35311809-2	2022	PLCbeta activity results in the hydrolysis of the membrane lipid phosphatidylinositol 4,5 bisphosphate (PI(4,5)P2) leading ultimately to the activation of transient receptor potential (TRP) and TRP like (TRPL) channels.	pi(4,5)p2	104-113	transient receptor potential-like	Drosophila melanogaster	204-208
2851103-7	1988	On ELISA, AM-2 and AM-212 bound only to PI(4,5)P2 and did not cross-react with other acidic phospholipids including PI(4)P.	pi(4,5)p2	40-49	adrenomedullin 2	Homo sapiens	10-14
35433697-6	2022	FGF2 translocation across the membrane is initiated through sequential interactions with the Na,K-ATPase, Tec kinase, and phosphoinositide PI(4,5)P2 at the inner plasma membrane leaflet.	pi(4,5)p2	139-148	fibroblast growth factor 2	Homo sapiens	0-4
35433697-7	2022	Whereas the first two are auxiliary factors of this pathway, the interaction of FGF2 with PI(4,5)P2 triggers the core mechanism of FGF2 membrane translocation.	pi(4,5)p2	90-99	fibroblast growth factor 2	Homo sapiens	80-84
35433697-7	2022	Whereas the first two are auxiliary factors of this pathway, the interaction of FGF2 with PI(4,5)P2 triggers the core mechanism of FGF2 membrane translocation.	pi(4,5)p2	90-99	fibroblast growth factor 2	Homo sapiens	131-135
35433697-8	2022	It is based on a lipidic membrane pore that is formed by PI(4,5)P2-induced oligomerization of FGF2.	pi(4,5)p2	57-66	fibroblast growth factor 2	Homo sapiens	94-98
35204765-6	2022	Recently, we demonstrated that HBD-2 binds strongly to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), a key mediator of defensin-induced cell death and an instructional messenger during cell migration.	pi(4,5)p2	94-103	defensin beta 4A	Homo sapiens	31-36
35044298-6	2022	We show that the binding of FCHo2 on cellular membranes promotes PI(4,5)P2 clustering at the boundary of cargo receptors and that this accumulation enhances clathrin assembly.	pi(4,5)p2	65-74	FCH and mu domain containing endocytic adaptor 2	Homo sapiens	28-33
35041619-5	2022	Further research revealed that OSBPL2 was located at the base of kinocilia in hair cells (HCs) and primary cilia in supporting cells (SCs), and functioned in the maintenance of ciliogenesis by regulating the homeostasis of PI(4,5)P2 on the cilia membrane.	pi(4,5)p2	223-232	oxysterol binding protein-like 2	Mus musculus	31-37
35041619-6	2022	OSBPL2 deficiency led to a significant increase of PI(4,5)P2 on the cilia membrane, which could be partially rescued by the overexpression of INPP5E.	pi(4,5)p2	51-60	oxysterol binding protein-like 2	Mus musculus	0-6
35041619-6	2022	OSBPL2 deficiency led to a significant increase of PI(4,5)P2 on the cilia membrane, which could be partially rescued by the overexpression of INPP5E.	pi(4,5)p2	51-60	inositol polyphosphate-5-phosphatase E	Homo sapiens	142-148
35121178-0	2022	Biochemical and NMR studies reveal specific interaction between STIMATE C-tail and PI(4,5)P2 or PI(3,4,5)P3-containing membrane.	pi(4,5)p2	83-92	STIM activating enhancer	Homo sapiens	64-71
35121178-4	2022	Here using liposome pulldown assay, we show that STIMATE-CT can specifically interact with PI(4,5)P2 or PI(3,4,5)P3-containing membrane.	pi(4,5)p2	91-100	STIM activating enhancer	Homo sapiens	49-56
33933440-2	2021	The nuclear receptor Steroidogenic Factor-1 (SF-1, NR5A1) binds the signaling phosphoinositides PI(4,5)P2 (PIP2) and PI(3,4,5)P3 (PIP3), and our previous crystal structures showed how the phosphoinositide headgroups regulate SF-1 function.	pi(4,5)p2	96-105	splicing factor 1	Homo sapiens	4-49
34054517-7	2021	Lipid-protein overlay experiments show that TRPM4 directly interacted with several anionic phospholipids, including PI(4,5)P2.	pi(4,5)p2	116-125	transient receptor potential cation channel, subfamily M, member 4	Mus musculus	44-49
34054517-8	2021	Depletion of PI(4,5)P2 with either wortmannin or PGE2 abrogated the stimulatory effects of exogenous cholesterol on TRPM4 activity, whereas exogenous PI(4,5)P2 (diC8-PI(4,5)P2, a water-soluble analog) increased the effects.	pi(4,5)p2	13-22	transient receptor potential cation channel, subfamily M, member 4	Mus musculus	116-121
34054517-9	2021	These results suggest that cholesterol stimulates TRPM4 via a PI(4,5)P2-dependent mechanism.	pi(4,5)p2	62-71	transient receptor potential cation channel, subfamily M, member 4	Mus musculus	50-55
33743346-3	2021	We previously reported that phospholipase C-related catalytically inactive protein (PRIP), a phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] binding protein, regulates PI3K/AKT signaling by competitively inhibiting substrate recognition by PI3K.	pi(4,5)p2	132-141	phospholipase C like 1 (inactive)	Homo sapiens	28-82
33743346-3	2021	We previously reported that phospholipase C-related catalytically inactive protein (PRIP), a phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] binding protein, regulates PI3K/AKT signaling by competitively inhibiting substrate recognition by PI3K.	pi(4,5)p2	132-141	phospholipase C like 1 (inactive)	Homo sapiens	84-88
33743346-3	2021	We previously reported that phospholipase C-related catalytically inactive protein (PRIP), a phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] binding protein, regulates PI3K/AKT signaling by competitively inhibiting substrate recognition by PI3K.	pi(4,5)p2	132-141	AKT serine/threonine kinase 1	Homo sapiens	175-178
33947796-1	2021	The dual-specificity phosphatase PTEN functions as a tumor suppressor by hydrolyzing PI(3,4,5)P3 to PI(4,5)P2 to inhibit PI3K-AKT signaling and cellular proliferation.	pi(4,5)p2	100-109	phosphatase and tensin homolog	Homo sapiens	33-37
33947796-1	2021	The dual-specificity phosphatase PTEN functions as a tumor suppressor by hydrolyzing PI(3,4,5)P3 to PI(4,5)P2 to inhibit PI3K-AKT signaling and cellular proliferation.	pi(4,5)p2	100-109	AKT serine/threonine kinase 1	Homo sapiens	126-129
33933440-2	2021	The nuclear receptor Steroidogenic Factor-1 (SF-1, NR5A1) binds the signaling phosphoinositides PI(4,5)P2 (PIP2) and PI(3,4,5)P3 (PIP3), and our previous crystal structures showed how the phosphoinositide headgroups regulate SF-1 function.	pi(4,5)p2	96-105	nuclear receptor subfamily 5 group A member 1	Homo sapiens	51-56
33933440-2	2021	The nuclear receptor Steroidogenic Factor-1 (SF-1, NR5A1) binds the signaling phosphoinositides PI(4,5)P2 (PIP2) and PI(3,4,5)P3 (PIP3), and our previous crystal structures showed how the phosphoinositide headgroups regulate SF-1 function.	pi(4,5)p2	96-105	splicing factor 1	Homo sapiens	45-49
33924946-0	2021	Species-Specific Regulation of TRPM2 by PI(4,5)P2 via the Membrane Interfacial Cavity.	pi(4,5)p2	40-49	transient receptor potential cation channel subfamily M member 2	Homo sapiens	31-36
33924946-3	2021	Our aim was to analyse the functional effect of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), commonly referred to as PIP2, on different TRPM2 orthologues.	pi(4,5)p2	87-96	transient receptor potential cation channel subfamily M member 2	Homo sapiens	142-147
33879605-6	2021	Applying PAR2 agonist initiated depletion of PI(4,5)P2, which then recovered during rapid receptor desensitization, giving way to endocytosis.	pi(4,5)p2	45-54	F2R like trypsin receptor 1	Homo sapiens	9-13
33879605-9	2021	Knock-down of beta-arrestin revealed that endogenous beta-arrestin normally doubles the rate of PIP5-kinase (PIP5K) after PAR2 desensitization, boosting PI(4,5)P2-dependent formation of clathrin-coated pits (CCPs) at the PM.	pi(4,5)p2	153-162	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	96-107
33879605-9	2021	Knock-down of beta-arrestin revealed that endogenous beta-arrestin normally doubles the rate of PIP5-kinase (PIP5K) after PAR2 desensitization, boosting PI(4,5)P2-dependent formation of clathrin-coated pits (CCPs) at the PM.	pi(4,5)p2	153-162	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	109-114
33879605-9	2021	Knock-down of beta-arrestin revealed that endogenous beta-arrestin normally doubles the rate of PIP5-kinase (PIP5K) after PAR2 desensitization, boosting PI(4,5)P2-dependent formation of clathrin-coated pits (CCPs) at the PM.	pi(4,5)p2	153-162	F2R like trypsin receptor 1	Homo sapiens	122-126
33879605-12	2021	Taken together, beta-arrestins can coordinate potentiation of PIP5K activity at CCPs to induce local PI(4,5)P2 generation that promotes recruitment of PI(4,5)P2-dependent endocytic machinery.	pi(4,5)p2	101-110	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	62-67
33879605-12	2021	Taken together, beta-arrestins can coordinate potentiation of PIP5K activity at CCPs to induce local PI(4,5)P2 generation that promotes recruitment of PI(4,5)P2-dependent endocytic machinery.	pi(4,5)p2	151-160	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	62-67
33920953-5	2021	The attenuation of endogenous PI(4,5)P2 levels by the activation of Danio rerio voltage-sensitive phosphatase (Dr-VSP) decreased the Cl- currents of TMEM16A(ac) but not the TMEM16A(a) isoform, which was abolished by the co-expression of PIP 5-kinase type-1gamma (PIPKIgamma).	pi(4,5)p2	30-39	transmembrane phosphatase with tensin homology	Danio rerio	68-109
33920953-5	2021	The attenuation of endogenous PI(4,5)P2 levels by the activation of Danio rerio voltage-sensitive phosphatase (Dr-VSP) decreased the Cl- currents of TMEM16A(ac) but not the TMEM16A(a) isoform, which was abolished by the co-expression of PIP 5-kinase type-1gamma (PIPKIgamma).	pi(4,5)p2	30-39	transmembrane phosphatase with tensin homology	Danio rerio	111-117
33920953-7	2021	Finally, we also confirmed that PI(4,5)P2 resynthesis is essential for TMEM16A(ac) recovery from Dr-VSP-induced current inhibition.	pi(4,5)p2	32-41	transmembrane phosphatase with tensin homology	Danio rerio	97-103
33918018-0	2021	The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus.	pi(4,5)p2	80-89	myosin phosphatase Rho interacting protein	Homo sapiens	20-25
33688074-3	2021	Here we show that, similar to the recently discovered polybasic polarity proteins such as Lgl and aPKC, Dlg also contains a positively charged polybasic domain that electrostatically binds the PM phosphoinositides PI4P and PI(4,5)P2 Electrostatic targeting by the polybasic domain contributes significantly to the PM localization of Dlg in follicular and early embryonic epithelial cells, and is crucial for Dlg to regulate both polarity and tumorigenesis.	pi(4,5)p2	223-232	atypical protein kinase C	Drosophila melanogaster	98-102
34007035-8	2021	We show that PI(4,5)P2 is required to recruit various endocytic proteins, including AP2-micro, to the plasma membrane, and thus to regulate clathrin-mediated endocytosis.	pi(4,5)p2	13-22	Integrase-type DNA-binding superfamily protein	Arabidopsis thaliana	84-87
34007035-9	2021	Finally, we find that inducible PI(4,5)P2 perturbation impacts the dynamics of the actin cytoskeleton as well as microtubule anisotropy.	pi(4,5)p2	32-41	actin-12	Arabidopsis thaliana	83-88
33841102-6	2021	One of these, the enzyme PLCgamma2 that hydrolyzes the PIP species PI(4,5)P2, displays altered expression in AD and PD and is currently being investigated as a potential therapeutic target.Perhaps unsurprisingly, neurodegenerative conditions exhibiting PIP dyshomeostasis also tend to show alterations in aspects of microglial function regulated by these lipids.	pi(4,5)p2	67-76	phospholipase C gamma 2	Homo sapiens	25-34
33841102-6	2021	One of these, the enzyme PLCgamma2 that hydrolyzes the PIP species PI(4,5)P2, displays altered expression in AD and PD and is currently being investigated as a potential therapeutic target.Perhaps unsurprisingly, neurodegenerative conditions exhibiting PIP dyshomeostasis also tend to show alterations in aspects of microglial function regulated by these lipids.	pi(4,5)p2	67-76	prolactin induced protein	Homo sapiens	55-58
33730593-0	2021	Synaptotagmin-1 interacts with PI(4,5)P2 to initiate synaptic vesicle docking in hippocampal neurons.	pi(4,5)p2	31-40	synaptotagmin I	Mus musculus	0-15
33730593-3	2021	Phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) can interact with Syt1 and SNAREs to contribute to vesicle exocytosis.	pi(4,5)p2	39-48	synaptotagmin I	Mus musculus	68-72
33730593-5	2021	Furthermore, we demonstrate that PI(4,5)P2 is the membrane partner of Syt1 to initiate SV docking, and disrupting their interaction could abolish the docking initiation.	pi(4,5)p2	33-42	synaptotagmin I	Mus musculus	70-74
33657369-0	2021	Hedgehog signaling and Tre1 regulate actin dynamics through PI(4,5)P2 to direct migration of Drosophila embryonic germ cells.	pi(4,5)p2	60-69	hedgehog	Drosophila melanogaster	0-8
33657369-0	2021	Hedgehog signaling and Tre1 regulate actin dynamics through PI(4,5)P2 to direct migration of Drosophila embryonic germ cells.	pi(4,5)p2	60-69	Trapped in endoderm 1	Drosophila melanogaster	23-27
33657369-0	2021	Hedgehog signaling and Tre1 regulate actin dynamics through PI(4,5)P2 to direct migration of Drosophila embryonic germ cells.	pi(4,5)p2	60-69	Actin 79B	Drosophila melanogaster	37-42
33657369-5	2021	We show that Tre1 is required for polarized accumulation of F-actin, PI(4,5)P2, and dPIP5K.	pi(4,5)p2	69-78	Trapped in endoderm 1	Drosophila melanogaster	13-17
32997199-5	2021	ENTH binds to membranes in a PI(4,5)P2-dependent manner but only induces curvature in the presence of PS.	pi(4,5)p2	29-38	clathrin interactor 1	Homo sapiens	0-4
33659254-8	2021	We further demonstrated that PI(4,5)P2 could rescue CFTR related defective intracellular GLUT4 transportation, and CFTR could regulate PI(4,5)P2 cellular level through PIP5KA, suggesting PI(4,5)P2 is a down-stream signal of CFTR.	pi(4,5)p2	135-144	cystic fibrosis transmembrane conductance regulator	Mus musculus	115-119
33659254-8	2021	We further demonstrated that PI(4,5)P2 could rescue CFTR related defective intracellular GLUT4 transportation, and CFTR could regulate PI(4,5)P2 cellular level through PIP5KA, suggesting PI(4,5)P2 is a down-stream signal of CFTR.	pi(4,5)p2	135-144	cystic fibrosis transmembrane conductance regulator	Mus musculus	52-56
33659254-8	2021	We further demonstrated that PI(4,5)P2 could rescue CFTR related defective intracellular GLUT4 transportation, and CFTR could regulate PI(4,5)P2 cellular level through PIP5KA, suggesting PI(4,5)P2 is a down-stream signal of CFTR.	pi(4,5)p2	135-144	cystic fibrosis transmembrane conductance regulator	Mus musculus	115-119
33659254-8	2021	We further demonstrated that PI(4,5)P2 could rescue CFTR related defective intracellular GLUT4 transportation, and CFTR could regulate PI(4,5)P2 cellular level through PIP5KA, suggesting PI(4,5)P2 is a down-stream signal of CFTR.	pi(4,5)p2	135-144	cystic fibrosis transmembrane conductance regulator	Mus musculus	115-119
33688074-3	2021	Here we show that, similar to the recently discovered polybasic polarity proteins such as Lgl and aPKC, Dlg also contains a positively charged polybasic domain that electrostatically binds the PM phosphoinositides PI4P and PI(4,5)P2 Electrostatic targeting by the polybasic domain contributes significantly to the PM localization of Dlg in follicular and early embryonic epithelial cells, and is crucial for Dlg to regulate both polarity and tumorigenesis.	pi(4,5)p2	223-232	discs large 1	Drosophila melanogaster	104-107
33574036-0	2021	A conserved PI(4,5)P2-binding domain is critical for immune regulatory function of DOCK8.	pi(4,5)p2	12-21	dedicator of cytokinesis 8	Homo sapiens	83-88
33574036-3	2021	We show that the DOCK homology region (DHR)-1 domain of DOCK8 binds specifically to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and is required for its recruitment to the plasma membrane.	pi(4,5)p2	123-132	dedicator of cytokinesis 8	Homo sapiens	56-61
33574036-7	2021	Thus, our study uncovers a critical role of DOCK8 in coupling PI(4,5)P2 signaling with Cdc42 activation for immune regulation.	pi(4,5)p2	62-71	dedicator of cytokinesis 8	Homo sapiens	44-49
33548224-7	2021	The absence of LRP1 reduces the levels of both PIP5KL1 and PIP5K1beta in the plasma membrane, and also lowers phosphatidylinositol(4,5) bisphosphate (PI(4,5)P2) levels in hepatocytes.	pi(4,5)p2	150-159	low density lipoprotein receptor-related protein 1	Mus musculus	15-19
33659254-8	2021	We further demonstrated that PI(4,5)P2 could rescue CFTR related defective intracellular GLUT4 transportation, and CFTR could regulate PI(4,5)P2 cellular level through PIP5KA, suggesting PI(4,5)P2 is a down-stream signal of CFTR.	pi(4,5)p2	29-38	cystic fibrosis transmembrane conductance regulator	Mus musculus	52-56
33659254-8	2021	We further demonstrated that PI(4,5)P2 could rescue CFTR related defective intracellular GLUT4 transportation, and CFTR could regulate PI(4,5)P2 cellular level through PIP5KA, suggesting PI(4,5)P2 is a down-stream signal of CFTR.	pi(4,5)p2	29-38	solute carrier family 2 (facilitated glucose transporter), member 4	Mus musculus	89-94
33659254-8	2021	We further demonstrated that PI(4,5)P2 could rescue CFTR related defective intracellular GLUT4 transportation, and CFTR could regulate PI(4,5)P2 cellular level through PIP5KA, suggesting PI(4,5)P2 is a down-stream signal of CFTR.	pi(4,5)p2	135-144	cystic fibrosis transmembrane conductance regulator	Mus musculus	52-56
33659254-8	2021	We further demonstrated that PI(4,5)P2 could rescue CFTR related defective intracellular GLUT4 transportation, and CFTR could regulate PI(4,5)P2 cellular level through PIP5KA, suggesting PI(4,5)P2 is a down-stream signal of CFTR.	pi(4,5)p2	135-144	cystic fibrosis transmembrane conductance regulator	Mus musculus	115-119
33548224-8	2021	These data indicate that LRP1 recruits PIP5KL1 and PIP5K1beta to the plasma membrane for PI(4,5)P2 biosynthesis.	pi(4,5)p2	89-98	low density lipoprotein receptor-related protein 1	Mus musculus	25-29
33548224-8	2021	These data indicate that LRP1 recruits PIP5KL1 and PIP5K1beta to the plasma membrane for PI(4,5)P2 biosynthesis.	pi(4,5)p2	89-98	phosphatidylinositol-4-phosphate 5-kinase-like 1	Mus musculus	39-46
33548224-8	2021	These data indicate that LRP1 recruits PIP5KL1 and PIP5K1beta to the plasma membrane for PI(4,5)P2 biosynthesis.	pi(4,5)p2	89-98	phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha	Mus musculus	51-61
33548224-9	2021	The lack of LRP1 reduces lipid kinase expression, leading to lower PI(4,5)P2 levels thereby decreasing the availability of this lipid metabolite in the cardiolipin biosynthesis pathway to cause cardiolipin reduction and the impairment in mitochondria homeostasis.	pi(4,5)p2	67-76	low density lipoprotein receptor-related protein 1	Mus musculus	12-16
33548224-10	2021	Taken together, the current study identifies another signaling mechanism by which LRP1 regulates cell functions: Binding and recruitment of PIP5KL1 and PIP5K1beta to the membrane for PI(4,5)P2 synthesis.	pi(4,5)p2	183-192	low density lipoprotein receptor-related protein 1	Mus musculus	82-86
33548224-10	2021	Taken together, the current study identifies another signaling mechanism by which LRP1 regulates cell functions: Binding and recruitment of PIP5KL1 and PIP5K1beta to the membrane for PI(4,5)P2 synthesis.	pi(4,5)p2	183-192	phosphatidylinositol-4-phosphate 5-kinase-like 1	Mus musculus	140-147
33548224-10	2021	Taken together, the current study identifies another signaling mechanism by which LRP1 regulates cell functions: Binding and recruitment of PIP5KL1 and PIP5K1beta to the membrane for PI(4,5)P2 synthesis.	pi(4,5)p2	183-192	phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha	Mus musculus	152-162
33479206-4	2021	Alt-RPL36 partially localizes to the endoplasmic reticulum, where it interacts with TMEM24, which transports the phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) precursor phosphatidylinositol from the endoplasmic reticulum to the plasma membrane.	pi(4,5)p2	152-161	ribosomal protein L36	Homo sapiens	4-9
33479206-4	2021	Alt-RPL36 partially localizes to the endoplasmic reticulum, where it interacts with TMEM24, which transports the phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) precursor phosphatidylinositol from the endoplasmic reticulum to the plasma membrane.	pi(4,5)p2	152-161	C2CD2 like	Homo sapiens	84-90
33479206-5	2021	Knock-out of alt-RPL36 increases plasma membrane PI(4,5)P2 levels, upregulates PI3K-AKT-mTOR signaling, and increases cell size.	pi(4,5)p2	49-58	ribosomal protein L36	Homo sapiens	17-22
32418222-9	2020	Phosphatidylinositol 4-kinases such as PI4K2A mediate the first step in the main metabolic pathway that generates PI4P, PI(4,5)P2 and PI(3,4,5)P3 .	pi(4,5)p2	120-129	phosphatidylinositol 4-kinase type 2 alpha	Homo sapiens	39-45
33408002-0	2021	Ethanol inhibits Kv7.2/7.3 channel open probability by reducing the PI(4,5)P2 sensitivity of Kv7.2 subunit.	pi(4,5)p2	68-77	potassium voltage-gated channel subfamily Q member 2	Homo sapiens	17-26
33408002-0	2021	Ethanol inhibits Kv7.2/7.3 channel open probability by reducing the PI(4,5)P2 sensitivity of Kv7.2 subunit.	pi(4,5)p2	68-77	potassium voltage-gated channel subfamily Q member 2	Homo sapiens	17-22
33408002-4	2021	Even though our studies revealed that the inhibitory effects of ethanol on the Kv7.2/7.3 channel was diminished by the increase of plasma membrane phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), the molecular mechanism of ethanol on Kv7.2/7.3 channel inhibition remains unclear.	pi(4,5)p2	186-195	potassium voltage-gated channel subfamily Q member 2	Homo sapiens	79-88
33408002-4	2021	Even though our studies revealed that the inhibitory effects of ethanol on the Kv7.2/7.3 channel was diminished by the increase of plasma membrane phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), the molecular mechanism of ethanol on Kv7.2/7.3 channel inhibition remains unclear.	pi(4,5)p2	186-195	potassium voltage-gated channel subfamily Q member 2	Homo sapiens	236-245
33481235-1	2021	Phosphatidylinositol 4-phosphate 5-kinase (PIP5K) is an enzyme that converts phosphatidylinositol 4-phosphate [PI4P] to phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	159-168	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	0-41
33481235-1	2021	Phosphatidylinositol 4-phosphate 5-kinase (PIP5K) is an enzyme that converts phosphatidylinositol 4-phosphate [PI4P] to phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	159-168	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	43-48
33481235-3	2021	In general, to investigate an enzymatic activity of PIP5K, the amount of incorporated [P32] ATP into PI(4,5)P2 fraction is measured in in vitro reconstitution experiments.	pi(4,5)p2	101-110	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	52-57
33334886-5	2021	We demonstrate that TRE are enriched in both phosphatidic acid (PA) and phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), supporting the idea of MICAL-L1 recruitment by PA and Rab10 recruitment via PI(4,5)P2.	pi(4,5)p2	111-120	MICAL like 1	Homo sapiens	146-154
33334886-5	2021	We demonstrate that TRE are enriched in both phosphatidic acid (PA) and phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), supporting the idea of MICAL-L1 recruitment by PA and Rab10 recruitment via PI(4,5)P2.	pi(4,5)p2	111-120	RAB10, member RAS oncogene family	Homo sapiens	177-182
33334886-5	2021	We demonstrate that TRE are enriched in both phosphatidic acid (PA) and phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), supporting the idea of MICAL-L1 recruitment by PA and Rab10 recruitment via PI(4,5)P2.	pi(4,5)p2	199-208	MICAL like 1	Homo sapiens	146-154
33172987-0	2020	Fission yeast Opy1 is an endogenous PI(4,5)P2 sensor that binds the PI5-kinase Its3.	pi(4,5)p2	36-45	Opy1p	Saccharomyces cerevisiae S288C	14-18
32418222-7	2020	The catalytic activity of PI4K2A in patient fibroblasts was severely reduced and lipid mass spectrometry showed that particular acyl-chain pools of PI4P and PI(4,5)P2 were decreased.	pi(4,5)p2	157-166	phosphatidylinositol 4-kinase type 2 alpha	Homo sapiens	26-32
33093276-0	2020	Dual regulatory effects of PI(4,5)P2 on TREK-2 K+ channel through antagonizing interaction between the alkaline residues (K330 and R355-357) in the cytosolic C-terminal helix.	pi(4,5)p2	27-36	potassium two pore domain channel subfamily K member 10	Homo sapiens	40-46
33574036-7	2021	Thus, our study uncovers a critical role of DOCK8 in coupling PI(4,5)P2 signaling with Cdc42 activation for immune regulation.	pi(4,5)p2	62-71	cell division cycle 42	Homo sapiens	87-92
33408002-7	2021	Finally, ethanol selectively facilitated the kinetics of Kv7.2 current suppression by voltage-sensing phosphatase (VSP)-induced PI(4,5)P2 depletion, while it slowed down Kv7.2 current recovery from the VSP-induced inhibition.	pi(4,5)p2	128-137	potassium voltage-gated channel subfamily Q member 2	Homo sapiens	57-62
33408002-8	2021	Together our results suggest that ethanol regulates neuronal activity through the reduction of open probability and PI(4,5)P2 sensitivity of Kv7.2/7.3 channels.	pi(4,5)p2	116-125	potassium voltage-gated channel subfamily Q member 2	Homo sapiens	141-148
33119550-3	2021	INPP5K hydrolyses phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) to phosphatidylinositol 4-phosphate (PI(4)P) and INPP5K mutations cause muscular dystrophy by unknown mechanisms.	pi(4,5)p2	57-66	inositol polyphosphate-5-phosphatase K	Homo sapiens	0-6
33119550-3	2021	INPP5K hydrolyses phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) to phosphatidylinositol 4-phosphate (PI(4)P) and INPP5K mutations cause muscular dystrophy by unknown mechanisms.	pi(4,5)p2	57-66	inositol polyphosphate-5-phosphatase K	Homo sapiens	117-123
33119550-5	2021	Reduced PI(4,5)P2 turnover on autolysosomes in Inpp5k-/- muscle suppresses autophagy and lysosome repopulation via ALR inhibition.	pi(4,5)p2	8-17	inositol polyphosphate-5-phosphatase K	Homo sapiens	47-53
33172987-6	2020	However, overexpression of Opy1 resulted in cytokinesis defects, as might be expected if it sequestered PI(4,5)P2 Our results highlight the evolutionary divergence of dual PH domain containing proteins and the need for caution when interpreting results based on their overexpression.	pi(4,5)p2	104-113	Opy1p	Saccharomyces cerevisiae S288C	27-31
32101066-3	2020	This review discusses recent findings on the role of PI(4,5)P2 and G-proteins in the modulation of TRPM8 upon receptor activation.	pi(4,5)p2	53-62	transient receptor potential cation channel subfamily M member 8	Homo sapiens	99-104
33199590-0	2020	Allosteric modulation of alternatively spliced Ca2+-activated Cl- channels TMEM16A by PI(4,5)P2 and CaMKII.	pi(4,5)p2	86-95	anoctamin 1	Homo sapiens	75-82
33199590-3	2020	We find that two splice variants of TMEM16A show different sensitivity to endogenous PI(4,5)P2 degradation, where TMEM16A(ac) displays higher channel activity and more current inhibition by PI(4,5)P2 depletion than TMEM16A(a).	pi(4,5)p2	85-94	anoctamin 1	Homo sapiens	36-43
33199590-3	2020	We find that two splice variants of TMEM16A show different sensitivity to endogenous PI(4,5)P2 degradation, where TMEM16A(ac) displays higher channel activity and more current inhibition by PI(4,5)P2 depletion than TMEM16A(a).	pi(4,5)p2	85-94	anoctamin 1	Homo sapiens	114-121
33199590-3	2020	We find that two splice variants of TMEM16A show different sensitivity to endogenous PI(4,5)P2 degradation, where TMEM16A(ac) displays higher channel activity and more current inhibition by PI(4,5)P2 depletion than TMEM16A(a).	pi(4,5)p2	85-94	anoctamin 1	Homo sapiens	114-121
33199590-3	2020	We find that two splice variants of TMEM16A show different sensitivity to endogenous PI(4,5)P2 degradation, where TMEM16A(ac) displays higher channel activity and more current inhibition by PI(4,5)P2 depletion than TMEM16A(a).	pi(4,5)p2	190-199	anoctamin 1	Homo sapiens	36-43
33199590-3	2020	We find that two splice variants of TMEM16A show different sensitivity to endogenous PI(4,5)P2 degradation, where TMEM16A(ac) displays higher channel activity and more current inhibition by PI(4,5)P2 depletion than TMEM16A(a).	pi(4,5)p2	190-199	anoctamin 1	Homo sapiens	114-121
33199590-3	2020	We find that two splice variants of TMEM16A show different sensitivity to endogenous PI(4,5)P2 degradation, where TMEM16A(ac) displays higher channel activity and more current inhibition by PI(4,5)P2 depletion than TMEM16A(a).	pi(4,5)p2	190-199	anoctamin 1	Homo sapiens	114-121
33199590-8	2020	In sum, our study reveals the following: differential regulation of alternatively spliced TMEM16A(ac) and (a) by plasma membrane PI(4,5)P2, modification of these effects by channel phosphorylation, identification of the molecular sites, and mechanistic explanation by in silico simulation.	pi(4,5)p2	129-138	anoctamin 1	Homo sapiens	90-97
32897354-4	2020	Here we show that Vav2-SH2 domain is a lipid-binding module that can recognize PI(4,5)P2 and PI(3,4,5)P3 lipids weakly but specifically.	pi(4,5)p2	79-88	vav guanine nucleotide exchange factor 2	Homo sapiens	18-22
32897354-5	2020	The specific lipid-binding site in Vav2-SH2 domain was identified by NMR chemical shift perturbation experiments using the head groups of PI(4,5)P2 and PI(3,4,5)P3, both of which bind to Vav2-SH2 with millimolar binding affinities.	pi(4,5)p2	138-147	vav guanine nucleotide exchange factor 2	Homo sapiens	35-39
32897354-5	2020	The specific lipid-binding site in Vav2-SH2 domain was identified by NMR chemical shift perturbation experiments using the head groups of PI(4,5)P2 and PI(3,4,5)P3, both of which bind to Vav2-SH2 with millimolar binding affinities.	pi(4,5)p2	138-147	vav guanine nucleotide exchange factor 2	Homo sapiens	187-191
32998886-3	2020	By combining nuclear magnetic resonance, neutron reflectometry, and molecular dynamics simulation, we show that binding of multiple PI(4,5)P2 molecules to the ASAP1 PH domain (i) triggers a functionally relevant allosteric conformational switch and (ii) maintains the PH domain in a well-defined orientation, allowing critical contacts with an Arf1 mimic to occur.	pi(4,5)p2	132-141	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1	Homo sapiens	159-164
32914503-1	2020	Oxysterol-binding protein-related protein 2 (ORP2), a cholesterol-PI(4,5)P2 countercurrent transporter, was recently identified as a novel regulator of plasma membrane (PM) cholesterol and PI(4,5)P2 content in HeLa cells.	pi(4,5)p2	66-75	oxysterol binding protein like 2	Homo sapiens	0-43
32914503-1	2020	Oxysterol-binding protein-related protein 2 (ORP2), a cholesterol-PI(4,5)P2 countercurrent transporter, was recently identified as a novel regulator of plasma membrane (PM) cholesterol and PI(4,5)P2 content in HeLa cells.	pi(4,5)p2	66-75	oxysterol binding protein like 2	Homo sapiens	45-49
32914503-1	2020	Oxysterol-binding protein-related protein 2 (ORP2), a cholesterol-PI(4,5)P2 countercurrent transporter, was recently identified as a novel regulator of plasma membrane (PM) cholesterol and PI(4,5)P2 content in HeLa cells.	pi(4,5)p2	189-198	oxysterol binding protein like 2	Homo sapiens	0-43
32914503-1	2020	Oxysterol-binding protein-related protein 2 (ORP2), a cholesterol-PI(4,5)P2 countercurrent transporter, was recently identified as a novel regulator of plasma membrane (PM) cholesterol and PI(4,5)P2 content in HeLa cells.	pi(4,5)p2	189-198	oxysterol binding protein like 2	Homo sapiens	45-49
32914503-2	2020	Here, we investigate the role of ORP2 in endothelial cell (EC) cholesterol and PI(4,5)P2 distribution, angiogenic signaling, and angiogenesis.	pi(4,5)p2	79-88	oxysterol binding protein like 2	Homo sapiens	33-37
32914503-6	2020	We demonstrate that ORP2 depletion increases the PM PI(4,5)P2 coincident with altered F-actin morphology, and reduces both VEGFR2 and cholesterol in buoyant raft membranes.	pi(4,5)p2	52-61	oxysterol binding protein like 2	Homo sapiens	20-24
32914503-10	2020	Our data identifies ORP2 as a novel regulator of EC cholesterol and PI(4,5)P2 homeostasis and cholesterol-dependent angiogenic signaling.	pi(4,5)p2	68-77	oxysterol binding protein-like 2	Mus musculus	20-24
32970792-5	2020	We show that Inp1 mediates peroxisome retention via an N-terminal domain that binds PI(4,5)P2 and a C-terminal Pex3-binding domain, forming a bridge between the peroxisomal membrane and the plasma membrane.	pi(4,5)p2	84-93	Inp1p	Saccharomyces cerevisiae S288C	13-17
32919786-1	2020	Loss-of-function mutations in the OCRL gene, which encodes the phosphatidylinositol [PI] 4,5-bisphosphate [PI(4,5)P2] 5-phosphatase OCRL, cause defective endocytosis and proximal tubule dysfunction in Lowe syndrome and Dent disease 2.	pi(4,5)p2	107-116	OCRL, inositol polyphosphate-5-phosphatase	Mus musculus	34-38
32919786-1	2020	Loss-of-function mutations in the OCRL gene, which encodes the phosphatidylinositol [PI] 4,5-bisphosphate [PI(4,5)P2] 5-phosphatase OCRL, cause defective endocytosis and proximal tubule dysfunction in Lowe syndrome and Dent disease 2.	pi(4,5)p2	107-116	OCRL, inositol polyphosphate-5-phosphatase	Mus musculus	132-136
32998886-2	2020	Binding of the pleckstrin homology (PH) domain of the ArfGAP With SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) to membranes containing phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is key for maximum GTP hydrolysis but not fully understood.	pi(4,5)p2	180-189	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1	Homo sapiens	54-108
32998886-2	2020	Binding of the pleckstrin homology (PH) domain of the ArfGAP With SH3 domain, ankyrin repeat and PH domain 1 (ASAP1) to membranes containing phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is key for maximum GTP hydrolysis but not fully understood.	pi(4,5)p2	180-189	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1	Homo sapiens	110-115
32998886-3	2020	By combining nuclear magnetic resonance, neutron reflectometry, and molecular dynamics simulation, we show that binding of multiple PI(4,5)P2 molecules to the ASAP1 PH domain (i) triggers a functionally relevant allosteric conformational switch and (ii) maintains the PH domain in a well-defined orientation, allowing critical contacts with an Arf1 mimic to occur.	pi(4,5)p2	132-141	ADP ribosylation factor 1	Homo sapiens	344-348
32998886-4	2020	Our model provides a framework to understand how binding of the ASAP1 PH domain to PI(4,5)P2 at the membrane may play a role in the regulation of ASAP1.	pi(4,5)p2	83-92	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1	Homo sapiens	64-69
32998886-4	2020	Our model provides a framework to understand how binding of the ASAP1 PH domain to PI(4,5)P2 at the membrane may play a role in the regulation of ASAP1.	pi(4,5)p2	83-92	ArfGAP with SH3 domain, ankyrin repeat and PH domain 1	Homo sapiens	146-151
32844214-2	2020	As a result, the functions of PI(4,5)P2 can be explored in two separate and distinct roles - as a substrate for phospholipase C (PLC) and phosphoinositide 3-kinase (PI3K) and as a primary messenger, each having unique properties.	pi(4,5)p2	30-39	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta	Homo sapiens	138-163
32532587-3	2020	Moreover, ligand-bound TRPM8 structures have uncovered unforeseen binding sites for both cooling agonists and membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	164-173	transient receptor potential cation channel subfamily M member 8	Homo sapiens	23-28
32535200-3	2020	The PI 5-phosphatase SHIP2 is a critical enzyme modulating PI(3,4,5)P3, PI(4,5)P2 and PI(3,4)P2 content in the cell.	pi(4,5)p2	72-81	inositol polyphosphate phosphatase like 1	Homo sapiens	21-26
32532587-4	2020	These complex structures unveil the molecular basis of cooling agonist sensing by TRPM8 and the allosteric role of PI(4,5)P2 in agonist binding for TRPM8 activation.	pi(4,5)p2	115-124	transient receptor potential cation channel subfamily M member 8	Homo sapiens	148-153
32826291-4	2020	Phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] becomes enriched at WPB-plasma membrane contact sites at the time of fusion, most likely downstream of phospholipase D1-mediated production of phosphatidic acid (PA) that activates phosphatidylinositol 4-phosphate (PI4P) 5-kinase gamma.	pi(4,5)p2	41-50	phospholipase D1	Homo sapiens	155-171
32356558-6	2020	Synj1+/- mice contain elevated 5"-phosphatase substrate, PI(4,5)P2, particularly in the midbrain neurons.	pi(4,5)p2	57-66	synaptojanin 1	Mus musculus	0-5
32811866-9	2020	The results strongly suggest that the regulation of ENaC is primarily exerted through the control of PI(4,5)P2 production by type-I phosphatidylinositol-4-phosphate 5-kinase (PIP5KI), which in turn is controlled by phosphatidic acid (PA), the product of the DGK reaction.	pi(4,5)p2	101-110	diacylglycerol kinase beta	Homo sapiens	258-261
32356558-7	2020	Moreover, pharmacological elevation of membrane PI(4,5)P2 in cultured neurons impairs SV endocytosis, specifically in midbrain neurons, and further exacerbates SV trafficking defects in Synj1+/- midbrain neurons.	pi(4,5)p2	48-57	synaptojanin 1	Mus musculus	186-191
32593716-7	2020	Global depletion of PI(4,5)P2 on the cell surface blunted insulin-stimulated Akt phosphorylation and abolished insulin effects in promotion of the docking and fusion of GLUT4 vesicle with the plasma membrane.	pi(4,5)p2	20-29	AKT serine/threonine kinase 1	Homo sapiens	77-80
32649209-4	2020	By variation of the lipid composition, we elucidated the role of PI(4,5)P2, cholesterol and an asymmetric sarcolemma composition for Bin1-N-BAR binding and sarcolemma tubulation.	pi(4,5)p2	65-74	bridging integrator 1	Homo sapiens	133-137
32649209-5	2020	Our results indicate that Bin1-N-BAR binding is low in the absence of PI(4,5)P2, and it is affected by additional changes in negative head group charge and lipid acyl chain composition.	pi(4,5)p2	70-79	bridging integrator 1	Homo sapiens	26-30
32752131-3	2020	Gag binding to the PM is regulated by cellular factors, including PM-specific phospholipid PI(4,5)P2 and tRNAs, both of which bind the highly basic region in the matrix domain of Gag.	pi(4,5)p2	91-100	Pr55(Gag)	Human immunodeficiency virus 1	0-3
32752131-3	2020	Gag binding to the PM is regulated by cellular factors, including PM-specific phospholipid PI(4,5)P2 and tRNAs, both of which bind the highly basic region in the matrix domain of Gag.	pi(4,5)p2	91-100	Pr55(Gag)	Human immunodeficiency virus 1	179-182
32593716-7	2020	Global depletion of PI(4,5)P2 on the cell surface blunted insulin-stimulated Akt phosphorylation and abolished insulin effects in promotion of the docking and fusion of GLUT4 vesicle with the plasma membrane.	pi(4,5)p2	20-29	insulin	Homo sapiens	111-118
32593716-7	2020	Global depletion of PI(4,5)P2 on the cell surface blunted insulin-stimulated Akt phosphorylation and abolished insulin effects in promotion of the docking and fusion of GLUT4 vesicle with the plasma membrane.	pi(4,5)p2	20-29	solute carrier family 2 member 4	Homo sapiens	169-174
32593716-8	2020	Furthermore, by development of a novel optogenetic module to selectively modulate PI(4,5)P2 levels on the GLUT4 vesicle docking site, we identified an important regulatory role of PI(4,5)P2 in controlling of vesicle docking process.	pi(4,5)p2	82-91	solute carrier family 2 member 4	Homo sapiens	106-111
32593716-8	2020	Furthermore, by development of a novel optogenetic module to selectively modulate PI(4,5)P2 levels on the GLUT4 vesicle docking site, we identified an important regulatory role of PI(4,5)P2 in controlling of vesicle docking process.	pi(4,5)p2	180-189	solute carrier family 2 member 4	Homo sapiens	106-111
32593716-9	2020	Local depletion of PI(4,5)P2 at the vesicle docking site promoted GLUT4 vesicle undocking, diminished insulin-stimulated GLUT4 vesicle docking and fusion, but without perturbation of insulin signaling propagation in adipocytes.	pi(4,5)p2	19-28	solute carrier family 2 member 4	Homo sapiens	66-71
32593716-9	2020	Local depletion of PI(4,5)P2 at the vesicle docking site promoted GLUT4 vesicle undocking, diminished insulin-stimulated GLUT4 vesicle docking and fusion, but without perturbation of insulin signaling propagation in adipocytes.	pi(4,5)p2	19-28	insulin	Homo sapiens	102-109
32593716-9	2020	Local depletion of PI(4,5)P2 at the vesicle docking site promoted GLUT4 vesicle undocking, diminished insulin-stimulated GLUT4 vesicle docking and fusion, but without perturbation of insulin signaling propagation in adipocytes.	pi(4,5)p2	19-28	solute carrier family 2 member 4	Homo sapiens	121-126
32593716-10	2020	Our results provide strong evidence that cell surface PI(4,5)P2 plays two distinct functions on regulation of the exocytic trafficking of GLUT4 in adipocytes.	pi(4,5)p2	54-63	solute carrier family 2 member 4	Homo sapiens	138-143
32593716-11	2020	PI(4,5)P2 not only regulates the proper activation of insulin signaling in general but also controls GLUT4 vesicle docking process at the vesicle-membrane contact sites.	pi(4,5)p2	0-9	insulin	Homo sapiens	54-61
32593716-11	2020	PI(4,5)P2 not only regulates the proper activation of insulin signaling in general but also controls GLUT4 vesicle docking process at the vesicle-membrane contact sites.	pi(4,5)p2	0-9	solute carrier family 2 member 4	Homo sapiens	101-106
32439160-5	2020	Here, PI(4,5)P2 was detected in the cell surface of non-permeabilized cells by anti-PI(4,5)P2 antibodies and the pleckstrin-homology (PH) domain of PLCdelta1 that specifically binds PI(4,5)P2.	pi(4,5)p2	6-15	phospholipase C delta 1	Homo sapiens	148-157
32593716-0	2020	Dual Function of PI(4,5)P2 in Insulin-Regulated Exocytic Trafficking of GLUT4 in Adipocytes.	pi(4,5)p2	17-26	insulin	Homo sapiens	30-37
32593716-0	2020	Dual Function of PI(4,5)P2 in Insulin-Regulated Exocytic Trafficking of GLUT4 in Adipocytes.	pi(4,5)p2	17-26	solute carrier family 2 member 4	Homo sapiens	72-77
32593716-2	2020	It has been implicated that phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is involved in insulin-regulated GLUT4 translocation in adipocytes.	pi(4,5)p2	67-76	insulin	Homo sapiens	93-100
32593716-2	2020	It has been implicated that phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] is involved in insulin-regulated GLUT4 translocation in adipocytes.	pi(4,5)p2	67-76	solute carrier family 2 member 4	Homo sapiens	111-116
32593716-5	2020	By combination of TIRFM imaging, we were able to monitor the temporal-spatial-dependent PI(4,5)P2 regulation on discrete steps of GLUT4 translocation in adipocytes.	pi(4,5)p2	88-97	solute carrier family 2 member 4	Homo sapiens	130-135
32593716-6	2020	We found that the plasma membrane localized PI(4,5)P2 is crucial for proper insulin signaling propagation and for insulin-stimulated GLUT4 vesicle translocation in 3T3-L1 adipocytes.	pi(4,5)p2	44-53	insulin	Homo sapiens	76-83
32593716-6	2020	We found that the plasma membrane localized PI(4,5)P2 is crucial for proper insulin signaling propagation and for insulin-stimulated GLUT4 vesicle translocation in 3T3-L1 adipocytes.	pi(4,5)p2	44-53	insulin	Homo sapiens	114-121
32593716-6	2020	We found that the plasma membrane localized PI(4,5)P2 is crucial for proper insulin signaling propagation and for insulin-stimulated GLUT4 vesicle translocation in 3T3-L1 adipocytes.	pi(4,5)p2	44-53	solute carrier family 2 member 4	Homo sapiens	133-138
32593716-7	2020	Global depletion of PI(4,5)P2 on the cell surface blunted insulin-stimulated Akt phosphorylation and abolished insulin effects in promotion of the docking and fusion of GLUT4 vesicle with the plasma membrane.	pi(4,5)p2	20-29	insulin	Homo sapiens	58-65
32686865-3	2020	Previous studies have demonstrated that the level of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in the membrane may potentially modulate Abeta production.	pi(4,5)p2	92-101	amyloid beta precursor protein	Homo sapiens	144-149
32686865-4	2020	Given that PI(4,5)P2 is produced by type 1 phosphatidylinositol-4-phosphate 5-kinases (PIP5Ks), we sought to determine whether the level of PIP5K type Ialpha (PIP5K1A) can affect production of Abeta by modulating the lipid composition of the membrane.	pi(4,5)p2	11-20	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	159-166
32439160-7	2020	Furthermore, blocking cell surface PI(4,5)P2 by the addition of anti-PI(4,5)P2 antibody or the PH domain of PLCdelta1 inhibited cell attachment, spreading, and migration.	pi(4,5)p2	35-44	phospholipase C delta 1	Homo sapiens	108-117
32376619-2	2020	Gag polyprotein precursors (Pr55Gag) are targeted to the PM and their binding is mediated by the interaction of myristoylated matrix domain and a PM-specific phosphoinositide, the phosphatidylinositol-(4,5)-bisphosphate (PI(4,5)P2).	pi(4,5)p2	221-230	Pr55(Gag)	Human immunodeficiency virus 1	28-35
32376619-3	2020	The major synthesis pathway of PI(4,5)P2 involves the activity of phosphatidylinositol 4-phosphate 5-kinase family type 1 composed of three isoforms (PIP5K1alpha, beta and gamma).	pi(4,5)p2	31-40	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	150-161
32376619-8	2020	Our findings demonstrated that, within the PIP5K1s family, only the PI(4,5)P2 pools produced by PIP5K1alpha and gamma are involved in Pr55Gag PM targeting process.IMPORTANCE PM specificity of Pr55Gag membrane binding is mediated through the interaction of PI(4,5)P2 with the MA basic residues.	pi(4,5)p2	68-77	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	96-107
32376619-8	2020	Our findings demonstrated that, within the PIP5K1s family, only the PI(4,5)P2 pools produced by PIP5K1alpha and gamma are involved in Pr55Gag PM targeting process.IMPORTANCE PM specificity of Pr55Gag membrane binding is mediated through the interaction of PI(4,5)P2 with the MA basic residues.	pi(4,5)p2	68-77	Pr55(Gag)	Human immunodeficiency virus 1	134-141
32376619-8	2020	Our findings demonstrated that, within the PIP5K1s family, only the PI(4,5)P2 pools produced by PIP5K1alpha and gamma are involved in Pr55Gag PM targeting process.IMPORTANCE PM specificity of Pr55Gag membrane binding is mediated through the interaction of PI(4,5)P2 with the MA basic residues.	pi(4,5)p2	68-77	Pr55(Gag)	Human immunodeficiency virus 1	192-199
32376619-8	2020	Our findings demonstrated that, within the PIP5K1s family, only the PI(4,5)P2 pools produced by PIP5K1alpha and gamma are involved in Pr55Gag PM targeting process.IMPORTANCE PM specificity of Pr55Gag membrane binding is mediated through the interaction of PI(4,5)P2 with the MA basic residues.	pi(4,5)p2	256-265	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	96-107
32376619-8	2020	Our findings demonstrated that, within the PIP5K1s family, only the PI(4,5)P2 pools produced by PIP5K1alpha and gamma are involved in Pr55Gag PM targeting process.IMPORTANCE PM specificity of Pr55Gag membrane binding is mediated through the interaction of PI(4,5)P2 with the MA basic residues.	pi(4,5)p2	256-265	Pr55(Gag)	Human immunodeficiency virus 1	134-141
32376619-8	2020	Our findings demonstrated that, within the PIP5K1s family, only the PI(4,5)P2 pools produced by PIP5K1alpha and gamma are involved in Pr55Gag PM targeting process.IMPORTANCE PM specificity of Pr55Gag membrane binding is mediated through the interaction of PI(4,5)P2 with the MA basic residues.	pi(4,5)p2	256-265	Pr55(Gag)	Human immunodeficiency virus 1	192-199
32376619-9	2020	It was shown that overexpression of a PI(4,5)P2-depleting enzyme strongly impaired plasma membrane (PM) localization of Pr55Gag However, cellular factors that control PI(4,5)P2-production required for Pr55Gag-PM targeting have not yet been characterized.	pi(4,5)p2	38-47	Pr55(Gag)	Human immunodeficiency virus 1	120-127
32376619-10	2020	In this study, by individually inhibiting PIP5K1 isoforms, we elucidated a correlation between PI(4,5)P2-metabolism pathways mediated by PIP5K1 isoforms and the targeting of Pr55Gag to the PM of TZM-bl HeLa cells.	pi(4,5)p2	95-104	Pr55(Gag)	Human immunodeficiency virus 1	174-181
32156686-7	2020	Furthermore, depleting PI(4,5)P2 in pip5k1 pip5k2 mutants inhibits fungal pathogen development and causes disease resistance, independent of cell death-associated defenses and involving impaired host susceptibility.	pi(4,5)p2	23-32	phosphatidylinositol-4-phosphate 5-kinase 1	Arabidopsis thaliana	36-42
32413098-2	2020	INPP4B dephosphorylates phospholipids at the 4th position of the inositol ring and inhibits AKT and PKC signaling by hydrolyzing of PI(3,4)P2 and PI(4,5)P2, respectively.	pi(4,5)p2	146-155	inositol polyphosphate-4-phosphatase, type II	Mus musculus	0-6
32188273-11	2020	Finally, knockdown increased hepatic PI(4,5)P2 levels in vivo and in HepG2 cells, while TMEM55B overexpression in vitro decreased PI(4,5)P2.	pi(4,5)p2	130-139	phosphatidylinositol-4,5-bisphosphate 4-phosphatase 1	Homo sapiens	88-95
32188273-13	2020	Notably, TMEM55B overexpression effect was reversed by incubation with PI(4,5)P2.	pi(4,5)p2	71-80	phosphatidylinositol-4,5-bisphosphate 4-phosphatase 1	Homo sapiens	9-16
32188273-14	2020	Conclusions: These findings indicate a role for TMEM55B in regulating plasma cholesterol levels by affecting PI(4,5)P2-mediated LDLR lysosomal degradation.	pi(4,5)p2	109-118	phosphatidylinositol-4,5-bisphosphate 4-phosphatase 1	Homo sapiens	48-55
32188273-14	2020	Conclusions: These findings indicate a role for TMEM55B in regulating plasma cholesterol levels by affecting PI(4,5)P2-mediated LDLR lysosomal degradation.	pi(4,5)p2	109-118	low density lipoprotein receptor	Homo sapiens	128-132
32086008-2	2020	ORP5 anchors at the endoplasmic reticulum (ER)-PM contact sites and acts as a PI(4)P and PI(4,5)P2/phosphatidylserine (PS) exchanger.	pi(4,5)p2	89-98	oxysterol binding protein-like 5	Mus musculus	0-4
32467162-0	2020	PI(4,5)P2-dependent regulation of exocytosis by amisyn, the vertebrate-specific competitor of synaptobrevin 2.	pi(4,5)p2	0-9	vesicle associated membrane protein 2	Homo sapiens	94-109
32345612-2	2020	Calcium influx through TRPV1 has been shown to activate a calcium-sensitive phospholipase C (PLC) enzyme and to lead to a robust decrease in phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] levels, which is a major contributor to channel desensitization.	pi(4,5)p2	180-189	transient receptor potential cation channel subfamily V member 1	Homo sapiens	23-28
32345612-3	2020	Diacylglycerol (DAG), the product of the PLC-catalyzed PI(4,5)P2 hydrolysis, activates protein kinase C (PKC).	pi(4,5)p2	55-64	proline rich transmembrane protein 2	Homo sapiens	87-103
32345612-3	2020	Diacylglycerol (DAG), the product of the PLC-catalyzed PI(4,5)P2 hydrolysis, activates protein kinase C (PKC).	pi(4,5)p2	55-64	proline rich transmembrane protein 2	Homo sapiens	105-108
32415087-0	2020	BIG1 controls macrophage pro-inflammatory responses through ARF3-mediated PI(4,5)P2 synthesis.	pi(4,5)p2	74-83	ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)	Mus musculus	0-4
32415087-0	2020	BIG1 controls macrophage pro-inflammatory responses through ARF3-mediated PI(4,5)P2 synthesis.	pi(4,5)p2	74-83	ADP-ribosylation factor 3	Mus musculus	60-64
32415087-6	2020	In bone marrow-derived macrophages or THP-1 cells, BIG1 deficiency caused an inhibited ARF3 activation, which reduced PI(4,5)P2 synthesis and the recruitment of TIRAP to the plasma membrane through inhibiting the activation of PIP5K induced by LPS, and eventually resulted in the inhibitory activity of TLR4-MyD88 signaling pathway.	pi(4,5)p2	118-127	ADP ribosylation factor guanine nucleotide exchange factor 1	Homo sapiens	51-55
32415087-6	2020	In bone marrow-derived macrophages or THP-1 cells, BIG1 deficiency caused an inhibited ARF3 activation, which reduced PI(4,5)P2 synthesis and the recruitment of TIRAP to the plasma membrane through inhibiting the activation of PIP5K induced by LPS, and eventually resulted in the inhibitory activity of TLR4-MyD88 signaling pathway.	pi(4,5)p2	118-127	ADP ribosylation factor 3	Homo sapiens	87-91
32167537-4	2020	For example, by applying simple changes in the membrane potential, Danio rerio VSP (Dr-VSP) has been used effectively to manipulate PI(4,5)P2 in mammalian cells with few, if any, side effects.	pi(4,5)p2	132-141	transmembrane phosphatase with tensin homology	Danio rerio	67-82
32167537-4	2020	For example, by applying simple changes in the membrane potential, Danio rerio VSP (Dr-VSP) has been used effectively to manipulate PI(4,5)P2 in mammalian cells with few, if any, side effects.	pi(4,5)p2	132-141	transmembrane phosphatase with tensin homology	Danio rerio	84-90
32167537-5	2020	In the present study, we report an enhanced version of Dr-VSP as an improved molecular tool for depleting PI(4,5)P2 from cultured mammalian cells.	pi(4,5)p2	106-115	transmembrane phosphatase with tensin homology	Danio rerio	55-61
32167537-9	2020	This modified Dr-VSP (CiDr-VSPmChe L223F, or what we call eVSP) induced more drastic voltage-evoked changes in PI(4,5)P2 levels, using the activities of Kir2.1, KCNQ2/3, and TRPC6 channels as functional readouts.	pi(4,5)p2	111-120	transmembrane phosphatase with tensin homology	Danio rerio	14-20
32167537-9	2020	This modified Dr-VSP (CiDr-VSPmChe L223F, or what we call eVSP) induced more drastic voltage-evoked changes in PI(4,5)P2 levels, using the activities of Kir2.1, KCNQ2/3, and TRPC6 channels as functional readouts.	pi(4,5)p2	111-120	potassium voltage-gated channel subfamily Q member 2	Homo sapiens	161-166
32167537-9	2020	This modified Dr-VSP (CiDr-VSPmChe L223F, or what we call eVSP) induced more drastic voltage-evoked changes in PI(4,5)P2 levels, using the activities of Kir2.1, KCNQ2/3, and TRPC6 channels as functional readouts.	pi(4,5)p2	111-120	transient receptor potential cation channel subfamily C member 6	Homo sapiens	174-179
32156686-7	2020	Furthermore, depleting PI(4,5)P2 in pip5k1 pip5k2 mutants inhibits fungal pathogen development and causes disease resistance, independent of cell death-associated defenses and involving impaired host susceptibility.	pi(4,5)p2	23-32	phosphatidylinositol-4-phosphate 5-kinase 2	Arabidopsis thaliana	43-49
31628071-13	2020	We asked whether SKIP and SHIP2 could have an impact on nuclear PI(4,5)P2.	pi(4,5)p2	64-73	inositol polyphosphate phosphatase like 1	Homo sapiens	26-31
32115406-5	2020	Mechanistically, osmotic stress induces PI(4,5)P2 plasma membrane enrichment by activating the PIP5K family, allowing for NF2 plasma membrane recruitment and subsequent downstream Hippo pathway activation.	pi(4,5)p2	40-49	NF2, moesin-ezrin-radixin like (MERLIN) tumor suppressor	Homo sapiens	122-125
32056006-3	2020	Phosphatidylinositol-4,5-bisphosphat (PI(4,5)P2)-dependent pathways are involved in the regulation of ABCB1 function.	pi(4,5)p2	38-47	ATP binding cassette subfamily B member 1	Homo sapiens	102-107
32056006-4	2020	The protein Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a pivotal regulator of PI(4,5)P2 and inactivated in many CRC cancers via genetic deletion or hyperphosphorylation.	pi(4,5)p2	93-102	myristoylated alanine rich protein kinase C substrate	Homo sapiens	12-57
32056006-4	2020	The protein Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a pivotal regulator of PI(4,5)P2 and inactivated in many CRC cancers via genetic deletion or hyperphosphorylation.	pi(4,5)p2	93-102	myristoylated alanine rich protein kinase C substrate	Homo sapiens	59-65
32198492-0	2020	Crystal structure of human PLD1 provides insight into activation by PI(4,5)P2 and RhoA.	pi(4,5)p2	68-77	phospholipase D1	Homo sapiens	27-31
32087576-6	2020	Expression of AtTSPO but not its N-terminally truncated variant enhanced phospholipase C activity and depleted PI(4,5)P2 from the plasma membrane and its enrichment in Golgi membranes.	pi(4,5)p2	111-120	TSPO(outer membrane tryptophan-rich sensory protein)-like protein	Arabidopsis thaliana	14-20
32214225-0	2020	The Na,K-ATPase acts upstream of phosphoinositide PI(4,5)P2 facilitating unconventional secretion of Fibroblast Growth Factor 2.	pi(4,5)p2	50-59	fibroblast growth factor 2	Homo sapiens	101-127
31825720-5	2020	To understand the underlying cause, we dissected the behavior of individual components of the Galphaq/PLCbeta/PI(4,5)P2 pathway during retraction and correlated these with the retraction of the membrane and cytoskeletal elements impacted by calcium signaling.	pi(4,5)p2	110-119	G protein subunit alpha q	Rattus norvegicus	94-101
31825720-8	2020	This study uncovers a novel mechanochemical connection between Galphaq/PLCbeta /PI(4,5)P2 that couples calcium responses with neural plasticity.	pi(4,5)p2	80-89	G protein subunit alpha q	Rattus norvegicus	63-70
32111929-0	2020	Single-molecule imaging of PI(4,5)P2 and PTEN in vitro reveals a positive feedback mechanism for PTEN membrane binding.	pi(4,5)p2	27-36	phosphatase and tensin homolog	Homo sapiens	97-101
32111929-3	2020	Here we developed an in vitro single-molecule imaging assay with various lipid compositions and use it to demonstrate that the enzymatic product, PI(4,5)P2, stabilizes PTEN"s membrane-binding.	pi(4,5)p2	146-155	phosphatase and tensin homolog	Homo sapiens	168-172
32111929-4	2020	The dissociation kinetics and lateral mobility of PTEN depended on the PI(4,5)P2 density on artificial lipid bilayers.	pi(4,5)p2	71-80	phosphatase and tensin homolog	Homo sapiens	50-54
32111929-7	2020	These results suggest that PI(4,5)P2-mediated positive feedback and PTEN-induced PI(4,5)P2 clustering may be important for anterior-posterior polarization.	pi(4,5)p2	81-90	phosphatase and tensin homolog	Homo sapiens	68-72
31825720-3	2020	In response to stimulation by neurotransmitters such as acetylcholine, calcium responses in cells are mediated by the Galphaq/phospholipase Cbeta (PLCbeta)/phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) signaling pathway.	pi(4,5)p2	195-204	G protein subunit alpha q	Rattus norvegicus	118-125
31913757-4	2020	TGFbeta induced rapid decreases in PI(4,5)P2 at the plasma membrane (PM) with increases in PI(4)P, followed by increases in PI(3,4)P2, in a TGFbeta receptor kinase ALK5-dependent manner.	pi(4,5)p2	35-44	transforming growth factor alpha	Homo sapiens	0-7
31913757-4	2020	TGFbeta induced rapid decreases in PI(4,5)P2 at the plasma membrane (PM) with increases in PI(4)P, followed by increases in PI(3,4)P2, in a TGFbeta receptor kinase ALK5-dependent manner.	pi(4,5)p2	35-44	transforming growth factor alpha	Homo sapiens	140-147
31913757-6	2020	Knockdown of synaptojanin1 abolished TGFbeta-induced PI(4,5)P2 decreases and PI(4)P increases.	pi(4,5)p2	53-62	synaptojanin 1	Homo sapiens	13-26
31913757-6	2020	Knockdown of synaptojanin1 abolished TGFbeta-induced PI(4,5)P2 decreases and PI(4)P increases.	pi(4,5)p2	53-62	transforming growth factor alpha	Homo sapiens	37-44
31913757-7	2020	Interestingly, PI3K-C2alpha KD abolished not only TGFbeta-induced PI(3,4)P2 increases but also TGFbeta-induced synaptojanin1 recruitment to the PM, PI(4,5)P2 decreases, and PI(4)P increases.	pi(4,5)p2	148-157	phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha	Homo sapiens	15-27
31913757-7	2020	Interestingly, PI3K-C2alpha KD abolished not only TGFbeta-induced PI(3,4)P2 increases but also TGFbeta-induced synaptojanin1 recruitment to the PM, PI(4,5)P2 decreases, and PI(4)P increases.	pi(4,5)p2	148-157	transforming growth factor alpha	Homo sapiens	95-102
31913757-7	2020	Interestingly, PI3K-C2alpha KD abolished not only TGFbeta-induced PI(3,4)P2 increases but also TGFbeta-induced synaptojanin1 recruitment to the PM, PI(4,5)P2 decreases, and PI(4)P increases.	pi(4,5)p2	148-157	synaptojanin 1	Homo sapiens	111-124
31640987-2	2019	The Gag-membrane interaction is mediated by binding of Gag"s N-terminal myristoylated matrix (MA) domain to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	147-156	Pr55(Gag)	Human immunodeficiency virus 1	4-7
31640987-2	2019	The Gag-membrane interaction is mediated by binding of Gag"s N-terminal myristoylated matrix (MA) domain to phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	147-156	Pr55(Gag)	Human immunodeficiency virus 1	55-58
31537645-10	2019	EGF induced hydrolysis of nuclear PI(4,5)P2 by the intranuclear PLCdelta4, rather than by PLCgamma1.	pi(4,5)p2	34-43	epidermal growth factor	Homo sapiens	0-3
31511376-10	2019	Notably, introduction of a heterologous multimerization domain restored PI(4,5)P2-dependent PM-specific localization for 29/31KR Gag-YFP, suggesting that the blocking of PM binding is more readily reversed than that of intracellular membrane binding.	pi(4,5)p2	72-81	Pr55(Gag)	Human immunodeficiency virus 1	129-132
31511376-12	2019	The interaction between the MA highly basic region (HBR) of Gag and the PM-specific lipid PI(4,5)P2 is critical for Gag localization to the PM.	pi(4,5)p2	90-99	Pr55(Gag)	Human immunodeficiency virus 1	60-63
31511376-12	2019	The interaction between the MA highly basic region (HBR) of Gag and the PM-specific lipid PI(4,5)P2 is critical for Gag localization to the PM.	pi(4,5)p2	90-99	Pr55(Gag)	Human immunodeficiency virus 1	116-119
31683182-1	2019	Recently there has been a flurry of interest in the regulation of the homo-dimeric calcium-activated chloride channel ANO1 (also known as TMEM16A) by phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2).	pi(4,5)p2	191-200	anoctamin 1	Homo sapiens	118-122
31683182-1	2019	Recently there has been a flurry of interest in the regulation of the homo-dimeric calcium-activated chloride channel ANO1 (also known as TMEM16A) by phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2).	pi(4,5)p2	191-200	anoctamin 1	Homo sapiens	138-145
31683182-2	2019	These recent studies show that upon Ca2+ binding, PI(4,5)P2 cooperates to maintain the conductive state of ANO1.	pi(4,5)p2	50-59	anoctamin 1	Homo sapiens	107-111
31537645-10	2019	EGF induced hydrolysis of nuclear PI(4,5)P2 by the intranuclear PLCdelta4, rather than by PLCgamma1.	pi(4,5)p2	34-43	phospholipase C delta 4	Homo sapiens	64-73
31515451-4	2019	ANO1 currents in excised inside-out patches activated by 270 nM Ca2+ at +100 mV are increased by exogenous PI(4,5)P2 with an EC50 = 1.24 microM.	pi(4,5)p2	107-116	anoctamin 1	Homo sapiens	0-4
31432619-6	2019	Rab35 additionally regulates ciliary PI(4,5)P2 levels and interacts with Arl13b.	pi(4,5)p2	37-46	RAB35, member RAS oncogene family	Homo sapiens	0-5
31515451-5	2019	The effect of PI(4,5)P2 is dependent on membrane voltage and Ca2+ and is explained by a stabilization of the ANO1 Ca2+-bound open state.	pi(4,5)p2	14-23	anoctamin 1	Homo sapiens	109-113
31515451-6	2019	Unbiased atomistic MD simulations with 1.4 mol% PI(4,5)P2 in a phosphatidylcholine bilayer identified 8 binding sites with significant probability of binding PI(4,5)P2 Three of these sites captured 85% of all ANO1-PI(4,5)P2 interactions.	pi(4,5)p2	48-57	anoctamin 1	Homo sapiens	209-213
31515451-6	2019	Unbiased atomistic MD simulations with 1.4 mol% PI(4,5)P2 in a phosphatidylcholine bilayer identified 8 binding sites with significant probability of binding PI(4,5)P2 Three of these sites captured 85% of all ANO1-PI(4,5)P2 interactions.	pi(4,5)p2	158-167	anoctamin 1	Homo sapiens	209-213
31515451-6	2019	Unbiased atomistic MD simulations with 1.4 mol% PI(4,5)P2 in a phosphatidylcholine bilayer identified 8 binding sites with significant probability of binding PI(4,5)P2 Three of these sites captured 85% of all ANO1-PI(4,5)P2 interactions.	pi(4,5)p2	158-167	anoctamin 1	Homo sapiens	209-213
31515451-8	2019	PI(4,5)P2 is stabilized by hydrogen bonding between amino acid side chains and phosphate/hydroxyl groups on PI(4,5)P2 Binding of PI(4,5)P2 alters the position of the cytoplasmic extension of TM6, which plays a crucial role in ANO1 channel gating, and increases the accessibility of the inner vestibule to Cl- ions.	pi(4,5)p2	0-9	anoctamin 1	Homo sapiens	226-230
31515451-9	2019	We propose a model consisting of a network of 3 PI(4,5)P2 binding sites at the cytoplasmic face of the membrane allosterically regulating ANO1 channel gating.	pi(4,5)p2	48-57	anoctamin 1	Homo sapiens	138-142
31144242-5	2019	Here, by combining high-resolution microscopic analyses and in vitro reconstitution of highly purified organelles or artificial liposomes, we demonstrate that peroxisomes form membrane contacts with the ER through the interaction between peroxisomal PI(4,5)P2 and ER-resident extended synaptotagmin-1, 2 and 3 (E-Syts).	pi(4,5)p2	250-259	extended synaptotagmin 1	Homo sapiens	276-309
31484968-3	2019	Here, we investigated whether phospholipase C (PLC)-related catalytically inactive protein (PRIP), a metabolic modulator of PI(4,5)P2, regulates PI(4,5)P2-mediated cytokinesis.	pi(4,5)p2	124-133	phospholipase C like 1 (inactive)	Homo sapiens	92-96
31484968-3	2019	Here, we investigated whether phospholipase C (PLC)-related catalytically inactive protein (PRIP), a metabolic modulator of PI(4,5)P2, regulates PI(4,5)P2-mediated cytokinesis.	pi(4,5)p2	145-154	phospholipase C like 1 (inactive)	Homo sapiens	92-96
31484968-6	2019	Importantly, PI(4,5)P2 accumulation at the cleavage furrow, as well as the localisation of RhoA and phospho-myosin II regulatory light chain to the cleavage furrow, were reduced in PRIP-silenced cells.	pi(4,5)p2	13-22	phospholipase C like 1 (inactive)	Homo sapiens	181-185
31484968-9	2019	Collectively, our results indicate that PRIP is a component at the cleavage furrow that maintains PI(4,5)P2 metabolism and regulates RhoA-dependent progression of cytokinesis.	pi(4,5)p2	98-107	phospholipase C like 1 (inactive)	Homo sapiens	40-44
31455163-3	2019	Here, we present a minimal mathematical model of ENaC, CFTR and ASL regulation that sheds light on the control of ENaC by the short palate lung and nasal epithelial clone 1 (SPLUNC1) protein and by phosphatidylinositol 4,5-biphosphate (PI(4,5)P2).	pi(4,5)p2	236-245	BPI fold containing family A member 1	Homo sapiens	174-181
31420534-4	2019	Mechanistically, WHAMM is recruited to the autolysosome membrane through its specific interaction with PI(4,5)P2.	pi(4,5)p2	103-112	WASP homolog associated with actin, golgi membranes and microtubules	Homo sapiens	17-22
31515451-2	2019	ANO1 is activated by elevation of cytosolic Ca2+ and modulated by phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	105-114	anoctamin 1	Homo sapiens	0-4
31515451-3	2019	Here, we describe a closely concerted experimental and computational study, including electrophysiology, mutagenesis, functional assays, and extended sampling of lipid-protein interactions with molecular dynamics (MD) to characterize PI(4,5)P2 binding modes and sites on ANO1.	pi(4,5)p2	234-243	anoctamin 1	Homo sapiens	271-275
31009484-3	2019	The Dyn1 PH domain has been shown to bind to lipids with a preference for PI(4,5)P2 and it is considered central to the function of Dyn1 in endocytosis.	pi(4,5)p2	74-83	dynein heavy chain	Saccharomyces cerevisiae S288C	4-8
31127000-0	2019	G alphaq Sensitizes TRPM8 to Inhibition by PI(4,5)P2 Depletion upon Receptor Activation.	pi(4,5)p2	43-52	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	20-25
31127000-4	2019	TRPM8 requires the plasma membrane phospholipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2 or PIP2] for activity.	pi(4,5)p2	87-96	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	0-5
31127000-6	2019	Here, we show that supplementing the whole-cell patch pipette with PI(4,5)P2 reduced inhibition of TRPM8 by activation of Galphaq-coupled receptors in mouse dorsal root ganglion (DRG) neurons isolated from both sexes.	pi(4,5)p2	67-76	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	99-104
31127000-8	2019	PI(4,5)P2 also reduced inhibition of TRPM8 by activation of heterologously expressed muscarinic M1 receptors.	pi(4,5)p2	0-9	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	37-42
31127000-9	2019	Coexpression of a constitutively active G alphaq protein that does not couple to PLC inhibited TRPM8 activity, and in cells expressing this protein, decreasing PI(4,5)P2 levels using a voltage-sensitive 5"-phosphatase induced a stronger inhibition of TRPM8 activity than in control cells.	pi(4,5)p2	160-169	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	251-256
31127000-10	2019	Our data indicate that, upon GPCR activation, G alphaq binding reduces the apparent affinity of TRPM8 for PI(4,5)P2 and thus sensitizes the channel to inhibition induced by decreasing PI(4,5)P2 levels.SIGNIFICANCE STATEMENT Increased sensitivity to heat in inflammation is partially mediated by inhibition of the cold- and menthol-sensitive transient receptor potential melastatin 8 (TRPM8) ion channels.	pi(4,5)p2	106-115	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	96-101
31127000-10	2019	Our data indicate that, upon GPCR activation, G alphaq binding reduces the apparent affinity of TRPM8 for PI(4,5)P2 and thus sensitizes the channel to inhibition induced by decreasing PI(4,5)P2 levels.SIGNIFICANCE STATEMENT Increased sensitivity to heat in inflammation is partially mediated by inhibition of the cold- and menthol-sensitive transient receptor potential melastatin 8 (TRPM8) ion channels.	pi(4,5)p2	106-115	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	341-382
31127000-10	2019	Our data indicate that, upon GPCR activation, G alphaq binding reduces the apparent affinity of TRPM8 for PI(4,5)P2 and thus sensitizes the channel to inhibition induced by decreasing PI(4,5)P2 levels.SIGNIFICANCE STATEMENT Increased sensitivity to heat in inflammation is partially mediated by inhibition of the cold- and menthol-sensitive transient receptor potential melastatin 8 (TRPM8) ion channels.	pi(4,5)p2	106-115	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	384-389
31127000-10	2019	Our data indicate that, upon GPCR activation, G alphaq binding reduces the apparent affinity of TRPM8 for PI(4,5)P2 and thus sensitizes the channel to inhibition induced by decreasing PI(4,5)P2 levels.SIGNIFICANCE STATEMENT Increased sensitivity to heat in inflammation is partially mediated by inhibition of the cold- and menthol-sensitive transient receptor potential melastatin 8 (TRPM8) ion channels.	pi(4,5)p2	184-193	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	96-101
31127000-10	2019	Our data indicate that, upon GPCR activation, G alphaq binding reduces the apparent affinity of TRPM8 for PI(4,5)P2 and thus sensitizes the channel to inhibition induced by decreasing PI(4,5)P2 levels.SIGNIFICANCE STATEMENT Increased sensitivity to heat in inflammation is partially mediated by inhibition of the cold- and menthol-sensitive transient receptor potential melastatin 8 (TRPM8) ion channels.	pi(4,5)p2	184-193	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	341-382
31127000-10	2019	Our data indicate that, upon GPCR activation, G alphaq binding reduces the apparent affinity of TRPM8 for PI(4,5)P2 and thus sensitizes the channel to inhibition induced by decreasing PI(4,5)P2 levels.SIGNIFICANCE STATEMENT Increased sensitivity to heat in inflammation is partially mediated by inhibition of the cold- and menthol-sensitive transient receptor potential melastatin 8 (TRPM8) ion channels.	pi(4,5)p2	184-193	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	384-389
31342668-4	2019	In the present study, we used two approaches to examine the effect of changing plasma membrane phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 ) levels on the activities of two electrogenic Na-Pi cotransporters, NaPi-IIa and NaPi-IIb.	pi(4,5)p2	134-143	solute carrier family 34 member 1	Homo sapiens	213-221
31342668-4	2019	In the present study, we used two approaches to examine the effect of changing plasma membrane phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2 ) levels on the activities of two electrogenic Na-Pi cotransporters, NaPi-IIa and NaPi-IIb.	pi(4,5)p2	134-143	solute carrier family 34 member 2	Homo sapiens	226-234
31061173-4	2019	Here, we identified Anoctamin 8 (ANO8) as a key tether in the formation of the ER/PM junctions that is essential for STIM1-STIM1 interaction and STIM1-Orai1 interaction and channel activation at a ER/PM PI(4,5)P2-rich compartment.	pi(4,5)p2	203-212	anoctamin 8	Homo sapiens	20-31
31061173-4	2019	Here, we identified Anoctamin 8 (ANO8) as a key tether in the formation of the ER/PM junctions that is essential for STIM1-STIM1 interaction and STIM1-Orai1 interaction and channel activation at a ER/PM PI(4,5)P2-rich compartment.	pi(4,5)p2	203-212	anoctamin 8	Homo sapiens	33-37
31084006-4	2019	Rather than driving membrane association of Gag, the presence of PI(4,5)P2 and the myristoyl group were found to anchor Gag at the membrane by decreasing the rate of desorption.	pi(4,5)p2	65-74	Pr55(Gag)	Human immunodeficiency virus 1	120-123
31084006-5	2019	Specifically, the interaction with PI(4,5)P2 allows Gag to overcome electrostatic repulsion with negatively charged lipids at the membrane surface.	pi(4,5)p2	35-44	Pr55(Gag)	Human immunodeficiency virus 1	52-55
31084006-6	2019	This behavior was verified by measuring the binding properties of Gag mutants in the matrix domain of Gag, which prevented anchoring to the membrane either by blocking interaction with PI(4,5)P2 or by preventing exposure of the myristoyl group.	pi(4,5)p2	185-194	Pr55(Gag)	Human immunodeficiency virus 1	66-69
31084006-6	2019	This behavior was verified by measuring the binding properties of Gag mutants in the matrix domain of Gag, which prevented anchoring to the membrane either by blocking interaction with PI(4,5)P2 or by preventing exposure of the myristoyl group.	pi(4,5)p2	185-194	Pr55(Gag)	Human immunodeficiency virus 1	102-105
31084006-7	2019	The presence of tRNA was found to inhibit Gag association with the membrane by specifically blocking the PI(4,5)P2 binding region, thereby preventing exposure of the myristoyl group and precluding subsequent anchoring of Gag to the membrane.	pi(4,5)p2	105-114	Pr55(Gag)	Human immunodeficiency virus 1	42-45
31084006-8	2019	While Gag likely samples all membranes, only the anchoring provided by the myristoyl group and PI(4,5)P2 allows Gag to accumulate at the membrane.	pi(4,5)p2	95-104	Pr55(Gag)	Human immunodeficiency virus 1	112-115
31091439-1	2019	Insulin stimulates the conversion of phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) to phosphatidylinositol-3,4,5-trisphosphate (PI(3,4,5)P3), which mediates downstream cellular responses.	pi(4,5)p2	76-85	insulin	Homo sapiens	0-7
31091439-3	2019	Here, we show that the loss of PIP4Ks (PIP4K2A, PIP4K2B, and PIP4K2C) in vitro results in a paradoxical increase in PI(4,5)P2 and a concomitant increase in insulin-stimulated production of PI(3,4,5)P3.	pi(4,5)p2	116-125	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	39-46
31091439-3	2019	Here, we show that the loss of PIP4Ks (PIP4K2A, PIP4K2B, and PIP4K2C) in vitro results in a paradoxical increase in PI(4,5)P2 and a concomitant increase in insulin-stimulated production of PI(3,4,5)P3.	pi(4,5)p2	116-125	phosphatidylinositol-5-phosphate 4-kinase type 2 beta	Homo sapiens	48-55
31091439-3	2019	Here, we show that the loss of PIP4Ks (PIP4K2A, PIP4K2B, and PIP4K2C) in vitro results in a paradoxical increase in PI(4,5)P2 and a concomitant increase in insulin-stimulated production of PI(3,4,5)P3.	pi(4,5)p2	116-125	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	61-68
31091439-6	2019	Our work uncovers an allosteric function of PIP4Ks in suppressing PIP5K-mediated PI(4,5)P2 synthesis and insulin-dependent conversion to PI(3,4,5)P3 and suggests that the pharmacological depletion of PIP4K enzymes could represent a strategy for enhancing insulin signaling.	pi(4,5)p2	81-90	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	66-71
31091453-3	2019	PLEKHA4 oligomerizes into clusters at PI(4,5)P2-rich regions of the plasma membrane and recruits the Cullin-3 (CUL3) E3 ubiquitin ligase substrate adaptor Kelch-like protein 12 (KLHL12) to these assemblies.	pi(4,5)p2	38-47	pleckstrin homology domain containing A4	Homo sapiens	0-7
31127000-13	2019	Here, we propose that direct binding of G alphaq both reduces TRPM8 activity and sensitizes the channel to inhibition by decreased levels of its cofactor, PI(4,5)P2 Our data demonstrate the convergence of two downstream effectors of receptor activation, G alphaq and PI(4,5)P2 hydrolysis, in the regulation of TRPM8.	pi(4,5)p2	155-164	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	62-67
31127000-13	2019	Here, we propose that direct binding of G alphaq both reduces TRPM8 activity and sensitizes the channel to inhibition by decreased levels of its cofactor, PI(4,5)P2 Our data demonstrate the convergence of two downstream effectors of receptor activation, G alphaq and PI(4,5)P2 hydrolysis, in the regulation of TRPM8.	pi(4,5)p2	155-164	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	310-315
31127000-13	2019	Here, we propose that direct binding of G alphaq both reduces TRPM8 activity and sensitizes the channel to inhibition by decreased levels of its cofactor, PI(4,5)P2 Our data demonstrate the convergence of two downstream effectors of receptor activation, G alphaq and PI(4,5)P2 hydrolysis, in the regulation of TRPM8.	pi(4,5)p2	267-276	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	62-67
31127000-13	2019	Here, we propose that direct binding of G alphaq both reduces TRPM8 activity and sensitizes the channel to inhibition by decreased levels of its cofactor, PI(4,5)P2 Our data demonstrate the convergence of two downstream effectors of receptor activation, G alphaq and PI(4,5)P2 hydrolysis, in the regulation of TRPM8.	pi(4,5)p2	267-276	transient receptor potential cation channel, subfamily M, member 8	Mus musculus	310-315
31339948-6	2019	In yeast, the subcellular localization of SopF is dependent on the activity of Mss4, a phosphatidylinositol 4-phosphate 5-kinase that generates PI(4,5)P2 from PI(4)P, indicating that membrane recruitment of SopF requires specific phospholipids.	pi(4,5)p2	144-153	1-phosphatidylinositol-4-phosphate 5-kinase	Saccharomyces cerevisiae S288C	79-83
31091438-4	2019	We find that PIP4K function at the plasma membrane enhances class I phosphoinositide 3-kinase (PI3K) activity, although the catalytic ability of PIP4K to produce phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] at the plasma membrane is dispensable for this regulation.	pi(4,5)p2	201-210	Phosphatidylinositol 5-phosphate 4-kinase	Drosophila melanogaster	13-18
31022885-7	2019	On the other hand, intracellular applications of PI(4,5)P2 recovered Ca2+-sensitivity of desensitized TRPM4, but its effect on TRPM5 was negligible.	pi(4,5)p2	49-58	transient receptor potential cation channel subfamily M member 4	Homo sapiens	102-107
31022885-8	2019	From these results, the Ca2+-binding sites of TRPM4 and TRPM5 were shown to be formed by the same amino acid residues by functional analyses, but the impact of PI(4,5)P2 on the regulation of TRPM5 seemed to be smaller than that on the regulation of TRPM4.	pi(4,5)p2	160-169	transient receptor potential cation channel subfamily M member 4	Homo sapiens	46-51
31022885-8	2019	From these results, the Ca2+-binding sites of TRPM4 and TRPM5 were shown to be formed by the same amino acid residues by functional analyses, but the impact of PI(4,5)P2 on the regulation of TRPM5 seemed to be smaller than that on the regulation of TRPM4.	pi(4,5)p2	160-169	transient receptor potential cation channel subfamily M member 5	Homo sapiens	56-61
31022885-8	2019	From these results, the Ca2+-binding sites of TRPM4 and TRPM5 were shown to be formed by the same amino acid residues by functional analyses, but the impact of PI(4,5)P2 on the regulation of TRPM5 seemed to be smaller than that on the regulation of TRPM4.	pi(4,5)p2	160-169	transient receptor potential cation channel subfamily M member 5	Homo sapiens	191-196
31009484-9	2019	Together our data indicate that the lipid binding capacity of the Insert B region of Vps1 contributes to the ability of the protein to associate with membranes and that its capacity to interact with PI(4,5)P2 is important in facilitating endocytic scission.	pi(4,5)p2	199-208	dynamin-like GTPase VPS1	Saccharomyces cerevisiae S288C	85-89
30590084-4	2019	ORP2 showed specific affinity for PI(4,5)P2, PI(3,4,5)P3 and PI(4)P, with suggestive Kd values in the muM range.	pi(4,5)p2	34-43	oxysterol binding protein like 2	Homo sapiens	0-4
30692200-5	2019	A proteomics screen revealed that focal adhesion kinase bound PI(4,5)P2, biochemical assays disclosed that focal adhesion kinase is preferentially activated by weak TCR signals and is required for optimal Treg induction, and further biochemical experiments revealed how TCR signaling strength regulates AKT activation.	pi(4,5)p2	62-71	AKT serine/threonine kinase 1	Homo sapiens	303-306
30692198-4	2019	Using a combination of biochemical, imaging, and cell biologic studies, combined with molecular dynamics and analytical theory, we test the hypothesis that the lateral distribution of PI(4,5)P2 within lipid membranes and native plasma membranes alters the capacity of PI(4,5)P2 to nucleate actin assembly in brain and neutrophil extracts and show that activities of formins and the Arp2/3 complex respond to PI(4,5)P2 lateral distribution.	pi(4,5)p2	184-193	actin related protein 2	Homo sapiens	382-386
30695232-7	2019	Upregulation of PI(4,5)P2 was observed in SKIP-depleted U-251 MG cells compared to control cells, but only when cells were adhered to fibronectin.	pi(4,5)p2	16-25	fibronectin 1	Homo sapiens	134-145
30783101-0	2019	Allosteric enhancement of ORP1-mediated cholesterol transport by PI(4,5)P2/PI(3,4)P2.	pi(4,5)p2	65-74	oxysterol binding protein like 1A	Homo sapiens	26-30
30670192-0	2019	The N-terminal homology (ENTH) domain of Epsin 1 is a sensitive reporter of physiological PI(4,5)P2 dynamics.	pi(4,5)p2	90-99	clathrin interactor 1	Homo sapiens	25-29
30670192-0	2019	The N-terminal homology (ENTH) domain of Epsin 1 is a sensitive reporter of physiological PI(4,5)P2 dynamics.	pi(4,5)p2	90-99	epsin 1	Homo sapiens	41-48
30670192-8	2019	ENTH-GFP specifically recognized membrane PI(4,5)P2 without interacting with IP3, as demonstrated by dialysis of cells with the messenger through a patch pipette.	pi(4,5)p2	42-51	clathrin interactor 1	Homo sapiens	0-4
30670192-9	2019	Utilizing Ci-VSP to titrate PI(4,5)P2 levels, we found that ENTH-GFP had low PI(4,5)P2 affinity.	pi(4,5)p2	28-37	clathrin interactor 1	Homo sapiens	60-64
30670192-9	2019	Utilizing Ci-VSP to titrate PI(4,5)P2 levels, we found that ENTH-GFP had low PI(4,5)P2 affinity.	pi(4,5)p2	77-86	clathrin interactor 1	Homo sapiens	60-64
30670192-10	2019	Accordingly, ENTH-GFP was highly sensitive to PLCbeta-dependent PI(4,5)P2 depletion, and in contrast to PLCdelta1-PH, overexpression of ENTH-GFP did not attenuate GqPCR signaling.	pi(4,5)p2	64-73	clathrin interactor 1	Homo sapiens	13-17
30670192-11	2019	Taken together, ENTH-GFP detects minute changes of PI(4,5)P2 levels and provides an important complementation of experimentally useful reporters of PI(4,5)P2 dynamics in physiological pathways.	pi(4,5)p2	51-60	clathrin interactor 1	Homo sapiens	16-20
30670192-11	2019	Taken together, ENTH-GFP detects minute changes of PI(4,5)P2 levels and provides an important complementation of experimentally useful reporters of PI(4,5)P2 dynamics in physiological pathways.	pi(4,5)p2	148-157	clathrin interactor 1	Homo sapiens	16-20
30783101-4	2019	Structures of the lipid-binding domain of ORP1 (ORP1-ORD) in complex with cholesterol or PI(4,5)P2 display open conformations essential for ORP function.	pi(4,5)p2	89-98	oxysterol binding protein like 1A	Homo sapiens	42-46
30783101-4	2019	Structures of the lipid-binding domain of ORP1 (ORP1-ORD) in complex with cholesterol or PI(4,5)P2 display open conformations essential for ORP function.	pi(4,5)p2	89-98	oxysterol binding protein like 1A	Homo sapiens	48-56
30765691-2	2019	Phospholipase C (PLC)s degrade phosphatidylinositol-4, 5-bisphosphate (PI(4,5)P2) lipids and regulate multiple cellular events.	pi(4,5)p2	71-80	perlecan (heparan sulfate proteoglycan 2)	Mus musculus	17-20
30765691-4	2019	Mechanistically, PI(4,5)P2 lipids directly interact with TAK1 at W241 and N245, and promote its activation.	pi(4,5)p2	17-26	mitogen-activated protein kinase kinase kinase 7	Mus musculus	57-61
30755645-0	2019	Differential PI(4,5)P2 sensitivities of TRPC4, C5 homomeric and TRPC1/4, C1/5 heteromeric channels.	pi(4,5)p2	13-22	trpc4	Danio rerio	40-45
30755645-0	2019	Differential PI(4,5)P2 sensitivities of TRPC4, C5 homomeric and TRPC1/4, C1/5 heteromeric channels.	pi(4,5)p2	13-22	transient receptor potential cation channel, subfamily C, member 1	Danio rerio	64-71
30755645-2	2019	Since phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) maintains TRPC4 and TRPC5 channel function, the Galphaq-PLC pathway inhibits channel activity by depleting PI(4,5)P2.	pi(4,5)p2	45-54	trpc4	Danio rerio	66-71
30755645-2	2019	Since phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) maintains TRPC4 and TRPC5 channel function, the Galphaq-PLC pathway inhibits channel activity by depleting PI(4,5)P2.	pi(4,5)p2	45-54	transient receptor potential cation channel, subfamily C, member 5a	Danio rerio	76-81
30755645-2	2019	Since phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) maintains TRPC4 and TRPC5 channel function, the Galphaq-PLC pathway inhibits channel activity by depleting PI(4,5)P2.	pi(4,5)p2	163-172	guanine nucleotide binding protein (G protein), q polypeptide	Danio rerio	104-111
30755645-7	2019	Thirdly, we determined putative PI(4,5)P2 binding sites based on a TRPC4 prediction model.	pi(4,5)p2	32-41	trpc4	Danio rerio	67-72
30755645-10	2019	We conclude that TRPC1 channel function as a regulator in setting PI(4,5)P2 affinity for TRPC4 and TRPC5 that changes PI(4,5)P2 sensitivity.	pi(4,5)p2	66-75	transient receptor potential cation channel, subfamily C, member 1	Danio rerio	17-22
30755645-10	2019	We conclude that TRPC1 channel function as a regulator in setting PI(4,5)P2 affinity for TRPC4 and TRPC5 that changes PI(4,5)P2 sensitivity.	pi(4,5)p2	66-75	trpc4	Danio rerio	89-94
30755645-10	2019	We conclude that TRPC1 channel function as a regulator in setting PI(4,5)P2 affinity for TRPC4 and TRPC5 that changes PI(4,5)P2 sensitivity.	pi(4,5)p2	66-75	transient receptor potential cation channel, subfamily C, member 5a	Danio rerio	99-104
30755645-10	2019	We conclude that TRPC1 channel function as a regulator in setting PI(4,5)P2 affinity for TRPC4 and TRPC5 that changes PI(4,5)P2 sensitivity.	pi(4,5)p2	118-127	transient receptor potential cation channel, subfamily C, member 1	Danio rerio	17-22
30755645-10	2019	We conclude that TRPC1 channel function as a regulator in setting PI(4,5)P2 affinity for TRPC4 and TRPC5 that changes PI(4,5)P2 sensitivity.	pi(4,5)p2	118-127	trpc4	Danio rerio	89-94
30755645-10	2019	We conclude that TRPC1 channel function as a regulator in setting PI(4,5)P2 affinity for TRPC4 and TRPC5 that changes PI(4,5)P2 sensitivity.	pi(4,5)p2	118-127	transient receptor potential cation channel, subfamily C, member 5a	Danio rerio	99-104
30581148-0	2019	ORP2 Delivers Cholesterol to the Plasma Membrane in Exchange for Phosphatidylinositol 4, 5-Bisphosphate (PI(4,5)P2).	pi(4,5)p2	105-114	oxysterol binding protein like 2	Homo sapiens	0-4
30581148-3	2019	Interestingly, ORP2-mediated enrichment of PM cholesterol was coupled with the removal of phosphatidylinositol 4, 5-bisphosphate (PI(4,5)P2) from the PM.	pi(4,5)p2	130-139	oxysterol binding protein like 2	Homo sapiens	15-19
30581148-4	2019	ORP2 overexpression or deficiency impacted the levels of PM cholesterol and PI(4,5)P2, and ORP2 efficiently transferred both cholesterol and PI(4,5)P2in vitro.	pi(4,5)p2	76-85	oxysterol binding protein like 2	Homo sapiens	0-4
30581148-4	2019	ORP2 overexpression or deficiency impacted the levels of PM cholesterol and PI(4,5)P2, and ORP2 efficiently transferred both cholesterol and PI(4,5)P2in vitro.	pi(4,5)p2	141-150	oxysterol binding protein like 2	Homo sapiens	0-4
30581148-4	2019	ORP2 overexpression or deficiency impacted the levels of PM cholesterol and PI(4,5)P2, and ORP2 efficiently transferred both cholesterol and PI(4,5)P2in vitro.	pi(4,5)p2	141-150	oxysterol binding protein like 2	Homo sapiens	91-95
30581148-5	2019	We determined the structure of ORP2 in complex with PI(4,5)P2 at 2.7 A resolution.	pi(4,5)p2	52-61	oxysterol binding protein like 2	Homo sapiens	31-35
30581148-6	2019	ORP2 formed a stable tetramer in the presence of PI(4,5)P2, and tetramerization was required for ORP2 to transfer PI(4,5)P2.	pi(4,5)p2	49-58	oxysterol binding protein like 2	Homo sapiens	0-4
30581148-6	2019	ORP2 formed a stable tetramer in the presence of PI(4,5)P2, and tetramerization was required for ORP2 to transfer PI(4,5)P2.	pi(4,5)p2	114-123	oxysterol binding protein like 2	Homo sapiens	0-4
30581148-6	2019	ORP2 formed a stable tetramer in the presence of PI(4,5)P2, and tetramerization was required for ORP2 to transfer PI(4,5)P2.	pi(4,5)p2	114-123	oxysterol binding protein like 2	Homo sapiens	97-101
30581148-7	2019	Our results identify a novel pathway for cholesterol delivery to the PM and establish ORP2 as a key regulator of both cholesterol and PI(4,5)P2 of the PM.	pi(4,5)p2	134-143	oxysterol binding protein like 2	Homo sapiens	86-90
30470711-3	2019	This process was dependent on both PI(4,5)P2-mediated recruitment of FGF2 at the inner leaflet and heparan sulfates capturing FGF2 at the outer plasma membrane leaflet.	pi(4,5)p2	35-44	fibroblast growth factor 2	Homo sapiens	69-73
30672465-5	2019	We show that PAR3 posterior plasma membrane exclusion depends on PAR1 and an endocytic mechanism relying on RAB5 and PI(4,5)P2.	pi(4,5)p2	117-126	bazooka	Drosophila melanogaster	13-17
30194087-4	2019	Except for inositol polyphosphate 5-phosphatase A, they all catalyze the dephosphorylation of PI(4,5)P2 and/or PI(3,4,5)P3 at the 5- position.	pi(4,5)p2	94-103	inositol polyphosphate-5-phosphatase A	Homo sapiens	11-49
30309983-1	2018	For most retroviruses, including HIV-1, binding of the Gag polyprotein to the plasma membrane (PM) is mediated by interactions between Gag"s N-terminal myristoylated matrix (MA) domain and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) in the PM.	pi(4,5)p2	228-237	Pr55(Gag)	Human immunodeficiency virus 1	55-58
30309983-1	2018	For most retroviruses, including HIV-1, binding of the Gag polyprotein to the plasma membrane (PM) is mediated by interactions between Gag"s N-terminal myristoylated matrix (MA) domain and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) in the PM.	pi(4,5)p2	228-237	Pr55(Gag)	Human immunodeficiency virus 1	135-138
29458182-8	2018	Based upon mutually exclusive interactions of FGF2 with PI(4,5)P2 versus heparan sulfates, an assembly/disassembly pathway has been proposed to be the underlying principle of directional transport of FGF2 across the plasma membrane.	pi(4,5)p2	56-65	fibroblast growth factor 2	Homo sapiens	46-50
30404949-5	2019	Cytohesin-1 isoforms, differing by the inclusion of an evolutionarily conserved three-nucleotide microexon in the pleckstrin homology domain, display differential affinity for PI(4,5)P2 (triglycine) and PI(3,4,5)P3 (diglycine).	pi(4,5)p2	176-185	cytohesin 1	Homo sapiens	0-11
30305398-10	2018	Cellular PI(4,5)P2 depletion increased the sensitivity of TRPM7 channels to the inhibitor 2-aminoethyl diphenyl borinate, which acidifies the cytosol.	pi(4,5)p2	9-18	transient receptor potential cation channel subfamily M member 7	Homo sapiens	58-63
30305398-13	2018	We conclude that the internal Mg2+-, polyamine-, and pH-mediated inhibition of TRPM7 channels is not direct but, rather, reflects electrostatic screening and resultant disruption of PI(4,5)P2-channel interactions.	pi(4,5)p2	182-191	transient receptor potential cation channel subfamily M member 7	Homo sapiens	79-84
29458182-8	2018	Based upon mutually exclusive interactions of FGF2 with PI(4,5)P2 versus heparan sulfates, an assembly/disassembly pathway has been proposed to be the underlying principle of directional transport of FGF2 across the plasma membrane.	pi(4,5)p2	56-65	fibroblast growth factor 2	Homo sapiens	200-204
30542582-3	2018	Here, we present a systematic study of the binding mechanism and specificity of a native agonist, phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and two of its variants, PI(3,4)P2 and PI(3,4,5)P3, on inwardly rectifying potassium channel Kir2.2, using molecular dynamics simulations and free energy perturbations (FEPs).	pi(4,5)p2	137-146	potassium inwardly rectifying channel subfamily J member 12	Homo sapiens	241-247
30420721-1	2018	Inositol polyphosphate multikinase (IPMK) is a member of the IPK-superfamily of kinases, catalyzing phosphorylation of several soluble inositols and the signaling phospholipid PI(4,5)P2 (PIP2).	pi(4,5)p2	176-185	inositol polyphosphate multikinase	Homo sapiens	0-34
30420721-1	2018	Inositol polyphosphate multikinase (IPMK) is a member of the IPK-superfamily of kinases, catalyzing phosphorylation of several soluble inositols and the signaling phospholipid PI(4,5)P2 (PIP2).	pi(4,5)p2	176-185	inositol polyphosphate multikinase	Homo sapiens	36-40
29987362-3	2018	Interestingly, PTEN shares remarkable homology to voltage-sensitive phosphatases (VSPs) that dephosphorylate D5 and D3 sites of PI(4,5)P2, PI(3,4)P2, and PI(3,4,5)P3.	pi(4,5)p2	128-137	phosphatase and tensin homolog	Homo sapiens	15-19
29987362-8	2018	Combining TI/ET mutations with an A126G exchange in the active site removed major sequence variations between PTEN and VSPs and resulted in D5 activity toward PI(4,5)P2 and PI(3,4,5)P3 of PTENCiV.	pi(4,5)p2	159-168	phosphatase and tensin homolog	Homo sapiens	110-114
30333133-7	2018	Localized phosphorylation is achieved through an intricate coincidence detection mechanism that requires the membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and the apically localized ezrin kinase, lymphocyte-oriented kinase (LOK, also known as STK10) or Ste20-like kinase (SLK).	pi(4,5)p2	163-172	serine/threonine kinase 10	Homo sapiens	243-246
30333133-7	2018	Localized phosphorylation is achieved through an intricate coincidence detection mechanism that requires the membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and the apically localized ezrin kinase, lymphocyte-oriented kinase (LOK, also known as STK10) or Ste20-like kinase (SLK).	pi(4,5)p2	163-172	serine/threonine kinase 10	Homo sapiens	262-267
30333133-7	2018	Localized phosphorylation is achieved through an intricate coincidence detection mechanism that requires the membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and the apically localized ezrin kinase, lymphocyte-oriented kinase (LOK, also known as STK10) or Ste20-like kinase (SLK).	pi(4,5)p2	163-172	STE20 like kinase	Homo sapiens	272-289
30333133-7	2018	Localized phosphorylation is achieved through an intricate coincidence detection mechanism that requires the membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and the apically localized ezrin kinase, lymphocyte-oriented kinase (LOK, also known as STK10) or Ste20-like kinase (SLK).	pi(4,5)p2	163-172	STE20 like kinase	Homo sapiens	291-294
30305626-3	2018	Both phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and calmodulin (CaM) have been shown to directly bind to TRPV5 and activate or inactivate the channel, respectively.	pi(4,5)p2	44-53	transient receptor potential cation channel subfamily V member 5	Homo sapiens	112-117
30542582-4	2018	Our results demonstrate that the major driving force for the PI(4,5)P2 specificity on Kir2.2 comes from the highly organized salt-bridge network formed between the charged inositol head and phosphodiester linker of PI(4,5)P2.	pi(4,5)p2	61-70	potassium inwardly rectifying channel subfamily J member 12	Homo sapiens	86-92
30542582-4	2018	Our results demonstrate that the major driving force for the PI(4,5)P2 specificity on Kir2.2 comes from the highly organized salt-bridge network formed between the charged inositol head and phosphodiester linker of PI(4,5)P2.	pi(4,5)p2	215-224	potassium inwardly rectifying channel subfamily J member 12	Homo sapiens	86-92
30012834-2	2018	SHIP2 (also known as INPPL1) is a phosphoinositide 5-phosphatase for phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2].	pi(4,5)p2	171-180	inositol polyphosphate phosphatase like 1	Homo sapiens	0-5
30012834-2	2018	SHIP2 (also known as INPPL1) is a phosphoinositide 5-phosphatase for phosphatidylinositol (3,4,5)-trisphosphate [PI(3,4,5)P3] and phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2].	pi(4,5)p2	171-180	inositol polyphosphate phosphatase like 1	Homo sapiens	21-27
30108272-3	2018	Here, we suggest that the regulation of TRPC1/4 and TRPC1/5 heterotetrameric channels by the Galphaq-PLCbeta pathway is self-limited and dynamically mediated by Galphaq and PI(4,5)P2.	pi(4,5)p2	173-182	transient receptor potential cation channel subfamily C member 1	Homo sapiens	40-47
30108272-3	2018	Here, we suggest that the regulation of TRPC1/4 and TRPC1/5 heterotetrameric channels by the Galphaq-PLCbeta pathway is self-limited and dynamically mediated by Galphaq and PI(4,5)P2.	pi(4,5)p2	173-182	transient receptor potential cation channel subfamily C member 1	Homo sapiens	52-59
30108272-3	2018	Here, we suggest that the regulation of TRPC1/4 and TRPC1/5 heterotetrameric channels by the Galphaq-PLCbeta pathway is self-limited and dynamically mediated by Galphaq and PI(4,5)P2.	pi(4,5)p2	173-182	G protein subunit alpha q	Homo sapiens	93-100
30108272-5	2018	Simultaneously, Galphaq-coupled PLCbeta activation leads to the breakdown of PI(4,5)P2, which inhibits activity of TRPC1/4 and 1/5 channels.	pi(4,5)p2	77-86	G protein subunit alpha q	Homo sapiens	16-23
29967034-5	2018	We establish that CHMP2B, both full-length and with a C-terminal deletion (DeltaC), preferentially binds to membranes containing phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	168-177	charged multivesicular body protein 2B	Homo sapiens	18-24
29980590-2	2018	PLC hydrolyzes the minor membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], and continued signaling requires the resynthesis and availability of PI(4,5)P2 at the plasma membrane.	pi(4,5)p2	79-88	Phospholipase C at 21C	Drosophila melanogaster	0-3
29980590-2	2018	PLC hydrolyzes the minor membrane lipid phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], and continued signaling requires the resynthesis and availability of PI(4,5)P2 at the plasma membrane.	pi(4,5)p2	160-169	Phospholipase C at 21C	Drosophila melanogaster	0-3
29980590-7	2018	Depletion of PI4KIIIalpha results in impaired electrical responses to light, and reduced plasma membrane levels of PI4P and PI(4,5)P2 Depletion of the conserved proteins Efr3 and TTC7 [also known as StmA and L(2)k14710, respectively, in flies], which assemble PI4KIIIalpha at the plasma membrane, also results in an impaired light response and reduced plasma membrane PI4P and PI(4,5)P2 levels.	pi(4,5)p2	124-133	Phosphatidylinositol 4-kinase III alpha	Drosophila melanogaster	13-25
29980590-7	2018	Depletion of PI4KIIIalpha results in impaired electrical responses to light, and reduced plasma membrane levels of PI4P and PI(4,5)P2 Depletion of the conserved proteins Efr3 and TTC7 [also known as StmA and L(2)k14710, respectively, in flies], which assemble PI4KIIIalpha at the plasma membrane, also results in an impaired light response and reduced plasma membrane PI4P and PI(4,5)P2 levels.	pi(4,5)p2	377-386	Phosphatidylinositol 4-kinase III alpha	Drosophila melanogaster	13-25
29980590-8	2018	Thus, PI4KIIIalpha activity at the plasma membrane generates PI4P and supports PI(4,5)P2 levels during receptor activated PLC signaling.	pi(4,5)p2	79-88	Phosphatidylinositol 4-kinase III alpha	Drosophila melanogaster	6-18
29980590-8	2018	Thus, PI4KIIIalpha activity at the plasma membrane generates PI4P and supports PI(4,5)P2 levels during receptor activated PLC signaling.	pi(4,5)p2	79-88	Phospholipase C at 21C	Drosophila melanogaster	122-125
29887380-7	2018	When bound to the membrane, FCHSD2 activates actin polymerization by a mechanism that combines oligomerization and recruitment of N-WASP to PI(4,5)P2, thus promoting pit maturation.	pi(4,5)p2	140-149	FCH and double SH3 domains 2	Homo sapiens	28-34
29887380-7	2018	When bound to the membrane, FCHSD2 activates actin polymerization by a mechanism that combines oligomerization and recruitment of N-WASP to PI(4,5)P2, thus promoting pit maturation.	pi(4,5)p2	140-149	WASP like actin nucleation promoting factor	Homo sapiens	130-136
30108272-5	2018	Simultaneously, Galphaq-coupled PLCbeta activation leads to the breakdown of PI(4,5)P2, which inhibits activity of TRPC1/4 and 1/5 channels.	pi(4,5)p2	77-86	transient receptor potential cation channel subfamily C member 1	Homo sapiens	115-122
29563216-1	2018	Arf6/ARF-6 is a crucial regulator of the endosomal phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) pool in endocytic recycling.	pi(4,5)p2	90-99	ADP-ribosylation factor 6	Caenorhabditis elegans	5-10
29563216-5	2018	PI(4,5)P2 levels and the endosomal labeling of the ARF-6 effector UNC-16 were significantly elevated in sac-1 mutants, suggesting that SAC-1 functions as a negative regulator of ARF-6.	pi(4,5)p2	0-9	phosphatidylinositol-3-phosphatase SAC1	Saccharomyces cerevisiae S288C	104-109
29563216-5	2018	PI(4,5)P2 levels and the endosomal labeling of the ARF-6 effector UNC-16 were significantly elevated in sac-1 mutants, suggesting that SAC-1 functions as a negative regulator of ARF-6.	pi(4,5)p2	0-9	phosphatidylinositol-3-phosphatase SAC1	Saccharomyces cerevisiae S288C	135-140
29563216-9	2018	BRIS-1 knockdown resulted in a significant reduction in PI(4,5)P2 levels in SAC-1-depleted cells.	pi(4,5)p2	56-65	phosphatidylinositol-3-phosphatase SAC1	Saccharomyces cerevisiae S288C	76-81
29469807-4	2018	GRAMD2a, but not GRAMD1a, co-localizes with the E-Syt2/3 tethers at ER-PM contacts in a PIP lipid-dependent manner and pre-marks the subset of PI(4,5)P2-enriched ER-PM MCSs utilized for STIM1 recruitment.	pi(4,5)p2	143-152	extended synaptotagmin 2	Homo sapiens	48-54
29472386-0	2018	PI(4,5)P2 controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites.	pi(4,5)p2	0-9	oxysterol binding protein like 5	Homo sapiens	58-62
29472386-3	2018	Regulation of the ORP5/8 attachment to the PM by both phosphoinositides provides a powerful means to determine the relative flux of PI4P toward the ER for PS transport and Sac1-mediated dephosphorylation and PIP 5-kinase-mediated conversion to PI(4,5)P2 Using this rheostat, cells can maintain PI(4,5)P2 levels by adjusting the availability of PI4P in the PM.	pi(4,5)p2	244-253	oxysterol binding protein like 5	Homo sapiens	18-24
29472386-3	2018	Regulation of the ORP5/8 attachment to the PM by both phosphoinositides provides a powerful means to determine the relative flux of PI4P toward the ER for PS transport and Sac1-mediated dephosphorylation and PIP 5-kinase-mediated conversion to PI(4,5)P2 Using this rheostat, cells can maintain PI(4,5)P2 levels by adjusting the availability of PI4P in the PM.	pi(4,5)p2	244-253	SAC1 like phosphatidylinositide phosphatase	Homo sapiens	172-176
29472386-3	2018	Regulation of the ORP5/8 attachment to the PM by both phosphoinositides provides a powerful means to determine the relative flux of PI4P toward the ER for PS transport and Sac1-mediated dephosphorylation and PIP 5-kinase-mediated conversion to PI(4,5)P2 Using this rheostat, cells can maintain PI(4,5)P2 levels by adjusting the availability of PI4P in the PM.	pi(4,5)p2	294-303	oxysterol binding protein like 5	Homo sapiens	18-24
29472386-3	2018	Regulation of the ORP5/8 attachment to the PM by both phosphoinositides provides a powerful means to determine the relative flux of PI4P toward the ER for PS transport and Sac1-mediated dephosphorylation and PIP 5-kinase-mediated conversion to PI(4,5)P2 Using this rheostat, cells can maintain PI(4,5)P2 levels by adjusting the availability of PI4P in the PM.	pi(4,5)p2	294-303	SAC1 like phosphatidylinositide phosphatase	Homo sapiens	172-176
29549788-3	2018	To characterize the interactions of wild type (WT) and DeltaMA Gag proteins with PI(4,5)P2 and other acidic phospholipids, we have employed a set of lipid biosensors as probes.	pi(4,5)p2	81-90	Pr55(Gag)	Human immunodeficiency virus 1	63-66
29363700-9	2018	Our studies demonstrate that membrane PI(4,5)P2 concentrations as low as 2.5-5 muM can trigger helix-coil conformational changes in gelsolin relevant for triggering regulatory processes in the cell.	pi(4,5)p2	38-47	gelsolin	Homo sapiens	132-140
29920471-6	2018	Extended-synaptotagmin 1 (E-Syt1) is recruited to the ER/SR-PM junction and anchors to the PM lipid phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) in a SOC-dependent manner.	pi(4,5)p2	139-148	extended synaptotagmin 1	Homo sapiens	0-24
29920471-6	2018	Extended-synaptotagmin 1 (E-Syt1) is recruited to the ER/SR-PM junction and anchors to the PM lipid phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) in a SOC-dependent manner.	pi(4,5)p2	139-148	extended synaptotagmin 1	Homo sapiens	26-32
29920471-7	2018	The subsequent strengthening of the ER/SR-PM connection by E-Syt1 facilitates the phosphatidylinositol (PI) transfer protein, Nir2, to supplement PI, a PI(4,5)P2 substrate, for the generation of IP3 and the propagation of Ca2+ signaling.	pi(4,5)p2	152-161	extended synaptotagmin 1	Homo sapiens	59-65
29920471-7	2018	The subsequent strengthening of the ER/SR-PM connection by E-Syt1 facilitates the phosphatidylinositol (PI) transfer protein, Nir2, to supplement PI, a PI(4,5)P2 substrate, for the generation of IP3 and the propagation of Ca2+ signaling.	pi(4,5)p2	152-161	phosphatidylinositol transfer protein membrane associated 1	Homo sapiens	126-130
29563576-4	2018	We observed that dephosphorylation of PI(4,5)P2 by the phosphatase increased the TCR/CD3 complex PM lateral mobility prior stimulation.	pi(4,5)p2	38-47	CD3 antigen, epsilon polypeptide	Mus musculus	85-88
29563576-6	2018	Using state-of-the-art biophotonic approaches, we further showed that PI(4,5)P2 dephosphorylation strongly promoted the CD3epsilon cytoplasmic domain unbinding from the PM inner leaflet in living cells, thus resulting in an increased CD3 availability for interactions with Lck kinase.	pi(4,5)p2	70-79	CD3 antigen, epsilon polypeptide	Mus musculus	120-123
29563576-6	2018	Using state-of-the-art biophotonic approaches, we further showed that PI(4,5)P2 dephosphorylation strongly promoted the CD3epsilon cytoplasmic domain unbinding from the PM inner leaflet in living cells, thus resulting in an increased CD3 availability for interactions with Lck kinase.	pi(4,5)p2	70-79	lymphocyte protein tyrosine kinase	Mus musculus	273-276
29563576-7	2018	This could significantly account for the observed effects of PI(4,5)P2 dephosphorylation on the CD3 phosphorylation.	pi(4,5)p2	61-70	CD3 antigen, epsilon polypeptide	Mus musculus	96-99
29500467-10	2018	The model suggests control strategies where the activities of the enzyme phosphoinositide 4-phosphate 5-kinase I (PIP5KI) or the PI4K + PIP5KI + DVL protein complex are decreased and cause an efficacious reduction in PI(4,5)P2 levels while avoiding undesirable alterations in other phosphoinositide pools.	pi(4,5)p2	217-226	dishevelled segment polarity protein 1 pseudogene 1	Homo sapiens	145-148
29223474-6	2018	SCDI mediated by SARAF depends on the location of the STIM1-Orai1 complex within a PI(4,5)P2-rich microdomain.	pi(4,5)p2	83-92	store-operated calcium entry associated regulatory factor	Homo sapiens	17-22
29223474-6	2018	SCDI mediated by SARAF depends on the location of the STIM1-Orai1 complex within a PI(4,5)P2-rich microdomain.	pi(4,5)p2	83-92	stromal interaction molecule 1	Homo sapiens	54-59
29223474-6	2018	SCDI mediated by SARAF depends on the location of the STIM1-Orai1 complex within a PI(4,5)P2-rich microdomain.	pi(4,5)p2	83-92	ORAI calcium release-activated calcium modulator 1	Homo sapiens	60-65
29277655-3	2018	Here we have studied the regulation of TMEM16A by phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), cholesterol, and fatty acids using patch clamp, biochemistry and fluorescence microscopy.	pi(4,5)p2	89-98	anoctamin 1	Homo sapiens	39-46
29277655-4	2018	We found that depletion of membrane PI(4,5)P2 causes a decline in TMEM16A current that is independent of cytoskeleton, but is partially prevented by removing intracellular Ca2+.	pi(4,5)p2	36-45	anoctamin 1	Homo sapiens	66-73
29277655-9	2018	Thus, PI(4,5)P2 is required for TMEM16A function while cholesterol directly and indirectly via a PI(4,5)P2-independent mechanism regulate channel function.	pi(4,5)p2	6-15	anoctamin 1	Homo sapiens	32-39
29440432-8	2018	Using an activity-based screen for ubiquitination targets, we identified multiple DTX1 substrates including PI5P4Kgamma, a lipid kinase involved in PI(4,5)P2 production.	pi(4,5)p2	148-157	deltex E3 ubiquitin ligase 1	Homo sapiens	82-86
29440432-13	2018	Additionally, they support a model where DTX1 controls Notch1 endosomal sorting decisions by controlling PI5P4Kgamma-mediated production of PI(4,5)P2.	pi(4,5)p2	140-149	deltex E3 ubiquitin ligase 1	Homo sapiens	41-45
29447222-10	2018	We developed a kinetic model to describe the observed reaction kinetics, which allowed us to i) distinguish between membrane-recruitment and allosteric activation of PTEN by PI(4,5)P2, ii) account for the influence of the biosensor on the observed reaction kinetics, and iii) demonstrate that all of these mechanisms contribute to the kinetics of PTEN-mediated catalysis.	pi(4,5)p2	174-183	phosphatase and tensin homolog	Homo sapiens	166-170
29440432-13	2018	Additionally, they support a model where DTX1 controls Notch1 endosomal sorting decisions by controlling PI5P4Kgamma-mediated production of PI(4,5)P2.	pi(4,5)p2	140-149	notch receptor 1	Homo sapiens	55-61
29447222-4	2018	One key enzyme that regulates PI(3,4,5)P3 levels at the plasma membrane is phosphatase and tensin homologue deleted on chromosome 10 (PTEN), which dephosphorylates PI(3,4,5)P3 through hydrolysis to form phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	242-251	phosphatase and tensin homolog	Homo sapiens	134-138
29447222-5	2018	It has been reported that PI(4,5)P2 is involved in positive feedback in the PI(3,4,5)P3 hydrolysis by PTEN.	pi(4,5)p2	26-35	phosphatase and tensin homolog	Homo sapiens	102-106
29426367-6	2018	Phosphatidylinositol 4,5-bisphosphate in PIP5K1alpha siRNA-mediated knockdown cells was also measured by the PI(4,5)P2 Mass ELISA Kit.	pi(4,5)p2	109-118	phosphatidylinositol-4-phosphate 5-kinase, type 1 alpha	Mus musculus	41-52
29415713-4	2018	RESULTS: Using biosensors for PI4P and PI(4,5)P2, the two most abundant PIPs at the plasma membrane, we reveal that meristem functions are associated with a stereotypical PIP tissue-scale pattern, with PI(4,5)P2 always displaying a more clear-cut pattern than PI4P.	pi(4,5)p2	39-48	proline iminopeptidase	Arabidopsis thaliana	72-75
29361542-7	2018	We document that GBF1 binds phosphoinositides, preferentially PI3P, PI4P and PI(4,5)P2, and that lipid binding requires the HDS1 domain.	pi(4,5)p2	77-86	golgi brefeldin A resistant guanine nucleotide exchange factor 1	Homo sapiens	17-21
29217618-3	2018	We have found earlier that upon LPS binding, CD14 triggers generation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a lipid controlling subsequent proinflammatory cytokine production.	pi(4,5)p2	112-121	toll-like receptor 4	Mus musculus	32-35
29217618-3	2018	We have found earlier that upon LPS binding, CD14 triggers generation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], a lipid controlling subsequent proinflammatory cytokine production.	pi(4,5)p2	112-121	CD14 antigen	Mus musculus	45-49
29217618-9	2018	Detailed studies of 17ODYA-labeled and immunoprecipitated proteins revealed that LPS induces S-palmitoylation, hence activation, of type II phosphatidylinositol 4-kinase (PI4KII) beta, which phosphorylates phosphatidylinositol to phosphatidylinositol 4-monophosphate, a PI(4,5)P2 precursor.	pi(4,5)p2	270-279	toll-like receptor 4	Mus musculus	81-84
29222176-4	2018	Using liposome-based assays, we show that Ca2+ binding to C2C promotes E-Syt1-mediated membrane tethering by releasing an inhibition that prevents C2E from interacting with PI(4,5)P2-rich membranes, as previously suggested by studies in semi-permeabilized cells.	pi(4,5)p2	173-182	extended synaptotagmin 1	Homo sapiens	71-77
29447222-10	2018	We developed a kinetic model to describe the observed reaction kinetics, which allowed us to i) distinguish between membrane-recruitment and allosteric activation of PTEN by PI(4,5)P2, ii) account for the influence of the biosensor on the observed reaction kinetics, and iii) demonstrate that all of these mechanisms contribute to the kinetics of PTEN-mediated catalysis.	pi(4,5)p2	174-183	phosphatase and tensin homolog	Homo sapiens	347-351
29353240-0	2018	PIP4K2A regulates intracellular cholesterol transport through modulating PI(4,5)P2 homeostasis.	pi(4,5)p2	73-82	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	0-7
29353240-3	2018	We found that knockdown of phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) reduced peroxisomal PI(4,5)P2 levels, decreased lysosome-peroxisome membrane contacts, and increased accumulation of lysosomal cholesterol in human SV-589 fibroblasts.	pi(4,5)p2	112-121	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	27-81
29353240-3	2018	We found that knockdown of phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) reduced peroxisomal PI(4,5)P2 levels, decreased lysosome-peroxisome membrane contacts, and increased accumulation of lysosomal cholesterol in human SV-589 fibroblasts.	pi(4,5)p2	112-121	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	83-90
29353240-5	2018	These results suggest that PIP4K2A plays a critical role in intracellular cholesterol transport by upregulating PI(4,5)P2 levels in the peroxisomal membrane.	pi(4,5)p2	112-121	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	27-34
28916189-0	2018	Phosphoinositide 5-phosphatase activities control cell motility in glioblastoma: Two phosphoinositides PI(4,5)P2 and PI(3,4)P2 are involved.	pi(4,5)p2	103-112	synaptojanin 2	Homo sapiens	0-30
28815958-9	2017	Both EXO70A1 and PATL3 were shown to bind PI4P and PI(4,5)P2 in vitro.	pi(4,5)p2	51-60	exocyst subunit exo70 family protein A1	Arabidopsis thaliana	5-12
29359392-5	2018	Recently, we have shown that HBD-3 can enter mammalian cells and bind to inner membrane phosphoinositide 4,5-bisphosphate [PI(4,5)P2], an important second lipid messenger of PLC and PI3K-Akt pathways.	pi(4,5)p2	123-132	defensin beta 103B	Homo sapiens	29-34
29359392-5	2018	Recently, we have shown that HBD-3 can enter mammalian cells and bind to inner membrane phosphoinositide 4,5-bisphosphate [PI(4,5)P2], an important second lipid messenger of PLC and PI3K-Akt pathways.	pi(4,5)p2	123-132	AKT serine/threonine kinase 1	Homo sapiens	187-190
29359392-6	2018	In this study, we report that the interaction of HBD-3 with PI(4,5)P2 is important for PI3K-Akt-NF-kappaBeta-mediated induction of tumor necrosis factor and interleukin-6.	pi(4,5)p2	60-69	defensin beta 103B	Homo sapiens	49-54
29359392-6	2018	In this study, we report that the interaction of HBD-3 with PI(4,5)P2 is important for PI3K-Akt-NF-kappaBeta-mediated induction of tumor necrosis factor and interleukin-6.	pi(4,5)p2	60-69	AKT serine/threonine kinase 1	Homo sapiens	92-95
29359392-6	2018	In this study, we report that the interaction of HBD-3 with PI(4,5)P2 is important for PI3K-Akt-NF-kappaBeta-mediated induction of tumor necrosis factor and interleukin-6.	pi(4,5)p2	60-69	interleukin 6	Homo sapiens	157-170
28916189-6	2018	Two other PIs, PI(4,5)P2 and PI(3,4)P2, are produced or used as substrates of PI 5-phosphatases (OCRL, INPP5B, SHIP1/2, SYNJ1/2, INPP5K, INPP5J, INPP5E).	pi(4,5)p2	15-24	OCRL inositol polyphosphate-5-phosphatase	Homo sapiens	97-101
28916189-6	2018	Two other PIs, PI(4,5)P2 and PI(3,4)P2, are produced or used as substrates of PI 5-phosphatases (OCRL, INPP5B, SHIP1/2, SYNJ1/2, INPP5K, INPP5J, INPP5E).	pi(4,5)p2	15-24	inositol polyphosphate-5-phosphatase B	Homo sapiens	103-109
28916189-6	2018	Two other PIs, PI(4,5)P2 and PI(3,4)P2, are produced or used as substrates of PI 5-phosphatases (OCRL, INPP5B, SHIP1/2, SYNJ1/2, INPP5K, INPP5J, INPP5E).	pi(4,5)p2	15-24	inositol polyphosphate-5-phosphatase D	Homo sapiens	111-118
28916189-6	2018	Two other PIs, PI(4,5)P2 and PI(3,4)P2, are produced or used as substrates of PI 5-phosphatases (OCRL, INPP5B, SHIP1/2, SYNJ1/2, INPP5K, INPP5J, INPP5E).	pi(4,5)p2	15-24	synaptojanin 1	Homo sapiens	120-127
28916189-6	2018	Two other PIs, PI(4,5)P2 and PI(3,4)P2, are produced or used as substrates of PI 5-phosphatases (OCRL, INPP5B, SHIP1/2, SYNJ1/2, INPP5K, INPP5J, INPP5E).	pi(4,5)p2	15-24	inositol polyphosphate-5-phosphatase K	Homo sapiens	129-135
28916189-6	2018	Two other PIs, PI(4,5)P2 and PI(3,4)P2, are produced or used as substrates of PI 5-phosphatases (OCRL, INPP5B, SHIP1/2, SYNJ1/2, INPP5K, INPP5J, INPP5E).	pi(4,5)p2	15-24	inositol polyphosphate-5-phosphatase J	Homo sapiens	137-143
28916189-6	2018	Two other PIs, PI(4,5)P2 and PI(3,4)P2, are produced or used as substrates of PI 5-phosphatases (OCRL, INPP5B, SHIP1/2, SYNJ1/2, INPP5K, INPP5J, INPP5E).	pi(4,5)p2	15-24	inositol polyphosphate-5-phosphatase E	Homo sapiens	145-151
28916189-10	2018	The two PI 5-phosphatases SHIP2 or SKIP could be localized at the plasma membrane and can reduce either PI(3,4,5)P3 or PI(4,5)P2 abundance.	pi(4,5)p2	119-128	inositol polyphosphate phosphatase like 1	Homo sapiens	26-31
28916189-11	2018	In the glioblastoma 1321 N1 cells, SHIP2 controls plasma membrane PI(4,5)P2 thereby participating in the control of cell migration.	pi(4,5)p2	66-75	inositol polyphosphate phosphatase like 1	Homo sapiens	35-40
29229838-2	2017	The first step in PM phosphoinositide synthesis is the conversion of phosphatidylinositol (PI) to PI4P, the precursor of PI(4,5)P2 and PI(3,4,5)P3 This conversion is catalyzed by the PI4KIIIalpha complex, comprising a lipid kinase, PI4KIIIalpha, and two regulatory subunits, TTC7 and FAM126.	pi(4,5)p2	121-130	tetratricopeptide repeat domain 7A	Homo sapiens	275-279
29073094-2	2017	Membrane phosphoinositides, especially phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], regulate the activities of many actin-binding proteins (ABPs), including profilin, cofilin, Dia2, N-WASP, ezrin, and moesin, but the underlying molecular mechanisms have remained elusive.	pi(4,5)p2	78-87	cofilin 1	Homo sapiens	173-180
29068313-4	2017	By combining this compound with electrophysiological measurements in mouse adrenal chromaffin cells, we show that PI(4,5)P2 uncaging potentiates exocytosis and identify synaptotagmin-1 (the Ca2+ sensor for exocytosis) and Munc13-2 (a vesicle priming protein) as the relevant effector proteins.	pi(4,5)p2	114-123	synaptotagmin I	Mus musculus	169-184
29068313-4	2017	By combining this compound with electrophysiological measurements in mouse adrenal chromaffin cells, we show that PI(4,5)P2 uncaging potentiates exocytosis and identify synaptotagmin-1 (the Ca2+ sensor for exocytosis) and Munc13-2 (a vesicle priming protein) as the relevant effector proteins.	pi(4,5)p2	114-123	unc-13 homolog B	Mus musculus	222-230
28923975-5	2017	Membrane curvature, PI(4,5)P2, and PI(3)P signals are needed for SNX9 assembly via its PX-BAR domain, whereas signaling through Cdc42 is activated by PI(4,5)P2 alone.	pi(4,5)p2	20-29	sorting nexin 9	Homo sapiens	65-69
28923975-5	2017	Membrane curvature, PI(4,5)P2, and PI(3)P signals are needed for SNX9 assembly via its PX-BAR domain, whereas signaling through Cdc42 is activated by PI(4,5)P2 alone.	pi(4,5)p2	150-159	cell division cycle 42	Homo sapiens	128-133
28799166-4	2017	FGF2 membrane translocation is mediated by the ability of FGF2 to oligomerise and to insert into the plasma membrane in a PI(4,5)P2 -dependent manner.	pi(4,5)p2	122-131	fibroblast growth factor 2	Homo sapiens	0-4
28799166-4	2017	FGF2 membrane translocation is mediated by the ability of FGF2 to oligomerise and to insert into the plasma membrane in a PI(4,5)P2 -dependent manner.	pi(4,5)p2	122-131	fibroblast growth factor 2	Homo sapiens	58-62
29073094-2	2017	Membrane phosphoinositides, especially phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], regulate the activities of many actin-binding proteins (ABPs), including profilin, cofilin, Dia2, N-WASP, ezrin, and moesin, but the underlying molecular mechanisms have remained elusive.	pi(4,5)p2	78-87	diaphanous related formin 2	Homo sapiens	182-186
29073094-2	2017	Membrane phosphoinositides, especially phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], regulate the activities of many actin-binding proteins (ABPs), including profilin, cofilin, Dia2, N-WASP, ezrin, and moesin, but the underlying molecular mechanisms have remained elusive.	pi(4,5)p2	78-87	WASP like actin nucleation promoting factor	Homo sapiens	188-194
29073094-2	2017	Membrane phosphoinositides, especially phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], regulate the activities of many actin-binding proteins (ABPs), including profilin, cofilin, Dia2, N-WASP, ezrin, and moesin, but the underlying molecular mechanisms have remained elusive.	pi(4,5)p2	78-87	moesin	Homo sapiens	207-213
29020973-6	2017	This effect was independent of the cAMP/PKA pathway and was mediated by the activation of the PI(4,5)P2/PLC pathway, a pathway which we have described to be triggered by the alphaMSH-dependent MC1R stimulation.	pi(4,5)p2	94-103	perlecan (heparan sulfate proteoglycan 2)	Mus musculus	104-107
28871046-0	2017	Loss of OCRL increases ciliary PI(4,5)P2 in Lowe oculocerebrorenal syndrome.	pi(4,5)p2	31-40	OCRL, inositol polyphosphate-5-phosphatase	Mus musculus	8-12
28871046-2	2017	Mutations in OCRL, an inositol polyphosphate 5-phosphatase that dephosphorylates PI(4,5)P2, cause Lowe syndrome.	pi(4,5)p2	81-90	OCRL, inositol polyphosphate-5-phosphatase	Mus musculus	13-17
28871046-6	2017	Accumulation of ciliary PI(4,5)P2 was pronounced in mouse embryonic fibroblasts (MEFs) derived from Lowe syndrome mouse model as well as in Ocrl-null MEFs, which was reversed by reintroduction of OCRL.	pi(4,5)p2	24-33	OCRL, inositol polyphosphate-5-phosphatase	Mus musculus	140-144
28871046-6	2017	Accumulation of ciliary PI(4,5)P2 was pronounced in mouse embryonic fibroblasts (MEFs) derived from Lowe syndrome mouse model as well as in Ocrl-null MEFs, which was reversed by reintroduction of OCRL.	pi(4,5)p2	24-33	OCRL, inositol polyphosphate-5-phosphatase	Mus musculus	196-200
29020973-6	2017	This effect was independent of the cAMP/PKA pathway and was mediated by the activation of the PI(4,5)P2/PLC pathway, a pathway which we have described to be triggered by the alphaMSH-dependent MC1R stimulation.	pi(4,5)p2	94-103	melanocortin 1 receptor	Mus musculus	193-197
29020973-14	2017	Meanwhile, 3 M3-FBS (3 M3), as an inducer of PI(4,5)P2/PLC pathway, reproduced the alphaMSH proliferative effect.	pi(4,5)p2	45-54	perlecan (heparan sulfate proteoglycan 2)	Mus musculus	55-58
28871048-4	2017	Under specific experimental conditions, secretion of IL-1beta and FGF2 is triggered by phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]-dependent formation of pores across the plasma membrane.	pi(4,5)p2	126-135	interleukin 1 beta	Homo sapiens	53-61
28871048-4	2017	Under specific experimental conditions, secretion of IL-1beta and FGF2 is triggered by phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2]-dependent formation of pores across the plasma membrane.	pi(4,5)p2	126-135	fibroblast growth factor 2	Homo sapiens	66-70
28871048-5	2017	However, the underlying mechanisms are different, with FGF2 known to directly interact with PI(4,5)P2, whereas in the case of IL-1beta secretion, it is proposed that the N-terminal fragment of gasdermin D interacts with PI(4,5)P2 to form the pore.	pi(4,5)p2	92-101	fibroblast growth factor 2	Homo sapiens	55-59
28871048-5	2017	However, the underlying mechanisms are different, with FGF2 known to directly interact with PI(4,5)P2, whereas in the case of IL-1beta secretion, it is proposed that the N-terminal fragment of gasdermin D interacts with PI(4,5)P2 to form the pore.	pi(4,5)p2	220-229	gasdermin D	Homo sapiens	193-204
28710365-9	2017	Collectively, PRIP regulates the production of PI(3,4,5)P3 from PI(4,5)P2 by PI3K, and the suppressor activity of PRIP in PI(4,5)P2 metabolism regulates the tumour migration, suggesting PRIP as a promising target for protection against metastatic progression.	pi(4,5)p2	122-131	phospholipase C like 1 (inactive)	Homo sapiens	114-118
28970821-10	2017	Since Syntenin-2 binds to PI(4,5)P2, we further tested whether the PI(4,5)P2 metabolic pathway might govern Syntenin-2 expression.	pi(4,5)p2	26-35	syndecan binding protein 2	Homo sapiens	6-16
28761175-4	2017	Exogenous addition of PI(4,5)P2 or phosphatidylinositol-4-phosphate 5-kinase (PIP5K) expression mimicked W13-generated-tubules which are inhibited by active Rac1.	pi(4,5)p2	22-31	Rac family small GTPase 1	Homo sapiens	157-161
28761175-6	2017	The results indicate that phospholipase C and ROCK1 are the main Rac1 effectors that impair plasma membrane invagination and tubule formation, essentially by decreasing PI(4,5)P2 levels and promoting cortical actomyosin assembly respectively.	pi(4,5)p2	169-178	Rho associated coiled-coil containing protein kinase 1	Homo sapiens	46-51
28761175-6	2017	The results indicate that phospholipase C and ROCK1 are the main Rac1 effectors that impair plasma membrane invagination and tubule formation, essentially by decreasing PI(4,5)P2 levels and promoting cortical actomyosin assembly respectively.	pi(4,5)p2	169-178	Rac family small GTPase 1	Homo sapiens	65-69
28722655-4	2017	FGF2 membrane translocation is thermodynamically driven by PI(4,5)P2-induced membrane insertion of FGF2 oligomers.	pi(4,5)p2	59-68	fibroblast growth factor 2	Homo sapiens	0-4
28722655-4	2017	FGF2 membrane translocation is thermodynamically driven by PI(4,5)P2-induced membrane insertion of FGF2 oligomers.	pi(4,5)p2	59-68	fibroblast growth factor 2	Homo sapiens	99-103
28722655-6	2017	Vectorial translocation of FGF2 across the membrane is governed by sequential and mutually exclusive interactions with PI(4,5)P2 and heparan sulfates on opposing sides of the membrane.	pi(4,5)p2	119-128	fibroblast growth factor 2	Homo sapiens	27-31
28722655-7	2017	Based on atomistic molecular dynamics simulations, we propose a mechanism that drives PI(4,5)P2 dependent oligomerization of FGF2.	pi(4,5)p2	86-95	fibroblast growth factor 2	Homo sapiens	125-129
28710365-5	2017	Overexpression of the PRIP pleckstrin homology domain, a PI(4,5)P2 binding motif, in MCF-7 cells caused significant suppression of cell migration.	pi(4,5)p2	57-66	phospholipase C like 1 (inactive)	Homo sapiens	22-26
28710365-8	2017	PI3K binding to PI(4,5)P2 was significantly inhibited by recombinant PRIP in vitro, and thus the activity of PI3K was downregulated.	pi(4,5)p2	16-25	phospholipase C like 1 (inactive)	Homo sapiens	69-73
28710365-9	2017	Collectively, PRIP regulates the production of PI(3,4,5)P3 from PI(4,5)P2 by PI3K, and the suppressor activity of PRIP in PI(4,5)P2 metabolism regulates the tumour migration, suggesting PRIP as a promising target for protection against metastatic progression.	pi(4,5)p2	64-73	phospholipase C like 1 (inactive)	Homo sapiens	14-18
28793264-0	2017	Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells.	pi(4,5)p2	41-50	insulin 1	Rattus norvegicus	90-95
28710365-9	2017	Collectively, PRIP regulates the production of PI(3,4,5)P3 from PI(4,5)P2 by PI3K, and the suppressor activity of PRIP in PI(4,5)P2 metabolism regulates the tumour migration, suggesting PRIP as a promising target for protection against metastatic progression.	pi(4,5)p2	122-131	phospholipase C like 1 (inactive)	Homo sapiens	114-118
28634262-0	2017	RASSF4: Regulator of plasma membrane PI(4,5)P2.	pi(4,5)p2	37-46	Ras association domain family member 4	Homo sapiens	0-6
28377218-1	2017	G-protein gated inwardly rectifying potassium (GIRK or Kir3) channels play a major role in the control of the heart rate, and require the membrane phospholipid phosphatidylinositol-bis-phosphate (PI(4,5)P2) for activation.	pi(4,5)p2	196-205	potassium inwardly rectifying channel subfamily J member 3 L homeolog	Xenopus laevis	47-51
28377218-4	2017	Here we employ planar lipid bilayers to investigate the crosstalk between PI(4,5)P2 and cholesterol, and demonstrate that these two lipids act synergistically to activate Kir3.4* currents.	pi(4,5)p2	74-83	potassium inwardly rectifying channel subfamily J member 5 L homeolog	Xenopus laevis	171-177
28634262-6	2017	https://doi.org/10.1083/jcb.201606047) demonstrate that RAS association domain family 4 (RASSF4) positively influences PI(4,5)P2 synthesis through ARF6-dependent regulation of PIP5K.	pi(4,5)p2	119-128	Ras association domain family member 4	Homo sapiens	56-87
28634262-6	2017	https://doi.org/10.1083/jcb.201606047) demonstrate that RAS association domain family 4 (RASSF4) positively influences PI(4,5)P2 synthesis through ARF6-dependent regulation of PIP5K.	pi(4,5)p2	119-128	Ras association domain family member 4	Homo sapiens	89-95
28634262-6	2017	https://doi.org/10.1083/jcb.201606047) demonstrate that RAS association domain family 4 (RASSF4) positively influences PI(4,5)P2 synthesis through ARF6-dependent regulation of PIP5K.	pi(4,5)p2	119-128	ADP ribosylation factor 6	Homo sapiens	147-151
28634262-6	2017	https://doi.org/10.1083/jcb.201606047) demonstrate that RAS association domain family 4 (RASSF4) positively influences PI(4,5)P2 synthesis through ARF6-dependent regulation of PIP5K.	pi(4,5)p2	119-128	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	176-181
28574338-2	2017	PM association of the main structural protein Gag depends on its myristoylated MA domain and PM PI(4,5)P2.	pi(4,5)p2	96-105	Pr55(Gag)	Human immunodeficiency virus 1	46-49
28574338-3	2017	Using a novel chemical biology tool that allows rapidly tunable manipulation of PI(4,5)P2 levels in living cells, we show that depletion of PI(4,5)P2 completely prevents Gag PM targeting and assembly site formation.	pi(4,5)p2	80-89	Pr55(Gag)	Human immunodeficiency virus 1	170-173
28574338-3	2017	Using a novel chemical biology tool that allows rapidly tunable manipulation of PI(4,5)P2 levels in living cells, we show that depletion of PI(4,5)P2 completely prevents Gag PM targeting and assembly site formation.	pi(4,5)p2	140-149	Pr55(Gag)	Human immunodeficiency virus 1	170-173
28574338-4	2017	Unexpectedly, PI(4,5)P2 depletion also caused loss of pre-assembled Gag lattices from the PM.	pi(4,5)p2	14-23	Pr55(Gag)	Human immunodeficiency virus 1	68-71
28507506-0	2017	Differential Regulation of PI(4,5)P2 Sensitivity of Kv7.2 and Kv7.3 Channels by Calmodulin.	pi(4,5)p2	27-36	calmodulin 3b (phosphorylase kinase, delta)	Danio rerio	80-90
27932583-2	2017	Gag recognizes phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2], a PM-specific phospholipid, via the highly basic region (HBR) in its N-terminal matrix (MA) domain.	pi(4,5)p2	56-65	Pr55(Gag)	Human immunodeficiency virus 1	0-3
28507506-5	2017	Current inhibition after transient depletion of the essential Kv7 co-factor phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) by activating Danio rerio voltage sensitive phosphatase (DrVSP) was blunted by co-expressing CaM1234 or the CaM sponge.	pi(4,5)p2	115-124	calmodulin 3b (phosphorylase kinase, delta)	Danio rerio	219-222
28507506-6	2017	In addition, CaM-dependent potentiation was occluded by tonic elevation of PI(4,5)P2 levels by PI(4)P5-kinase (PIP5K) expression.	pi(4,5)p2	75-84	calmodulin 3b (phosphorylase kinase, delta)	Danio rerio	13-16
28507506-8	2017	Sensitivity to PI(4,5)P2 depletion of Kv7.2/3 channels was increased after expression of CaM1234 or the CaM sponge, while that of homomeric Kv7.3 was unaltered.	pi(4,5)p2	15-24	calmodulin 3b (phosphorylase kinase, delta)	Danio rerio	89-92
28507506-9	2017	Altogether, the data reveal that apo-CaM influences PI(4,5)P2 dependence of Kv7.2, Kv7.2/3, and of Kv7.3 channels in a subunit specific manner.	pi(4,5)p2	52-61	calmodulin 3b (phosphorylase kinase, delta)	Danio rerio	37-40
28167678-6	2017	We propose that lipid remodeling mediated by the TORC2-Ypk1/Ypk2-Lem3 axis is a backup mechanism for PM anchoring of Rho1 after PM stress-induced acute degradation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], which is responsible for Rho1 localization under normal conditions.	pi(4,5)p2	206-215	serine/threonine protein kinase YPK1	Saccharomyces cerevisiae S288C	55-59
28167678-6	2017	We propose that lipid remodeling mediated by the TORC2-Ypk1/Ypk2-Lem3 axis is a backup mechanism for PM anchoring of Rho1 after PM stress-induced acute degradation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], which is responsible for Rho1 localization under normal conditions.	pi(4,5)p2	206-215	putative protein kinase YPK2	Saccharomyces cerevisiae S288C	60-64
28167678-6	2017	We propose that lipid remodeling mediated by the TORC2-Ypk1/Ypk2-Lem3 axis is a backup mechanism for PM anchoring of Rho1 after PM stress-induced acute degradation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], which is responsible for Rho1 localization under normal conditions.	pi(4,5)p2	206-215	Lem3p	Saccharomyces cerevisiae S288C	65-69
28167678-6	2017	We propose that lipid remodeling mediated by the TORC2-Ypk1/Ypk2-Lem3 axis is a backup mechanism for PM anchoring of Rho1 after PM stress-induced acute degradation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], which is responsible for Rho1 localization under normal conditions.	pi(4,5)p2	206-215	Rho family GTPase RHO1	Saccharomyces cerevisiae S288C	117-121
28154160-5	2017	We propose a three-step model for TULP3/TUB-mediated ciliary trafficking, including the capture of diverse membrane cargo by the tubby domain in a phosphoinositide 4,5-bisphosphate (PI(4,5)P2)-dependent manner, ciliary delivery by intraflagellar transport complex A binding to the TULP3/TUB N terminus, and subsequent release into PI(4,5)P2-deficient ciliary membrane.	pi(4,5)p2	182-191	TUB like protein 3	Homo sapiens	34-39
28154160-5	2017	We propose a three-step model for TULP3/TUB-mediated ciliary trafficking, including the capture of diverse membrane cargo by the tubby domain in a phosphoinositide 4,5-bisphosphate (PI(4,5)P2)-dependent manner, ciliary delivery by intraflagellar transport complex A binding to the TULP3/TUB N terminus, and subsequent release into PI(4,5)P2-deficient ciliary membrane.	pi(4,5)p2	182-191	TUB bipartite transcription factor	Homo sapiens	40-43
28154160-5	2017	We propose a three-step model for TULP3/TUB-mediated ciliary trafficking, including the capture of diverse membrane cargo by the tubby domain in a phosphoinositide 4,5-bisphosphate (PI(4,5)P2)-dependent manner, ciliary delivery by intraflagellar transport complex A binding to the TULP3/TUB N terminus, and subsequent release into PI(4,5)P2-deficient ciliary membrane.	pi(4,5)p2	182-191	TUB like protein 3	Homo sapiens	281-286
28154160-5	2017	We propose a three-step model for TULP3/TUB-mediated ciliary trafficking, including the capture of diverse membrane cargo by the tubby domain in a phosphoinositide 4,5-bisphosphate (PI(4,5)P2)-dependent manner, ciliary delivery by intraflagellar transport complex A binding to the TULP3/TUB N terminus, and subsequent release into PI(4,5)P2-deficient ciliary membrane.	pi(4,5)p2	182-191	TUB bipartite transcription factor	Homo sapiens	287-290
28154160-5	2017	We propose a three-step model for TULP3/TUB-mediated ciliary trafficking, including the capture of diverse membrane cargo by the tubby domain in a phosphoinositide 4,5-bisphosphate (PI(4,5)P2)-dependent manner, ciliary delivery by intraflagellar transport complex A binding to the TULP3/TUB N terminus, and subsequent release into PI(4,5)P2-deficient ciliary membrane.	pi(4,5)p2	331-340	TUB like protein 3	Homo sapiens	34-39
28154160-5	2017	We propose a three-step model for TULP3/TUB-mediated ciliary trafficking, including the capture of diverse membrane cargo by the tubby domain in a phosphoinositide 4,5-bisphosphate (PI(4,5)P2)-dependent manner, ciliary delivery by intraflagellar transport complex A binding to the TULP3/TUB N terminus, and subsequent release into PI(4,5)P2-deficient ciliary membrane.	pi(4,5)p2	331-340	TUB bipartite transcription factor	Homo sapiens	40-43
28473699-2	2017	OCRL1 encodes an inositol polyphosphate 5-phosphatase which preferentially dephosphorylates phosphatidylinositide 4,5 bisphosphate (PI(4,5)P2).	pi(4,5)p2	132-141	OCRL inositol polyphosphate-5-phosphatase	Homo sapiens	0-5
28177616-3	2017	Here, we study the direct interaction of Ca2+ with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2), the main lipid marker of the plasma membrane.	pi(4,5)p2	90-99	carbonic anhydrase 2	Homo sapiens	41-44
28177616-4	2017	Electrokinetic potential measurements of PI(4,5)P2 containing liposomes reveal that Ca2+ as well as Mg2+ reduce the zeta potential of liposomes to nearly background levels of pure phosphatidylcholine membranes.	pi(4,5)p2	41-50	carbonic anhydrase 2	Homo sapiens	84-87
28177616-5	2017	Strikingly, lipid recognition by the default PI(4,5)P2 lipid sensor, phospholipase C delta 1 pleckstrin homology domain (PLC delta1-PH), is completely inhibited in the presence of Ca2+, while Mg2+ has no effect with 100 nm liposomes and modest effect with giant unilamellar vesicles.	pi(4,5)p2	45-54	phospholipase C delta 1	Homo sapiens	69-92
28177616-5	2017	Strikingly, lipid recognition by the default PI(4,5)P2 lipid sensor, phospholipase C delta 1 pleckstrin homology domain (PLC delta1-PH), is completely inhibited in the presence of Ca2+, while Mg2+ has no effect with 100 nm liposomes and modest effect with giant unilamellar vesicles.	pi(4,5)p2	45-54	carbonic anhydrase 2	Homo sapiens	180-183
28222177-3	2017	In the autoinhibited conformation the FERM domain associates with the catalytic domain of FAK and PI(4,5)P2 binding to the FERM domain plays a role in the release of autoinhibition, activating the enzyme.	pi(4,5)p2	98-107	protein tyrosine kinase 2	Homo sapiens	90-93
28222177-4	2017	An in silico model of FAK/PI(4,5)P2 interaction suggests that residues on the catalytic domain interact with PI(4,5)P2, in addition to the known FERM domain PI(4,5)P2 binding site.	pi(4,5)p2	26-35	protein tyrosine kinase 2	Homo sapiens	22-25
28222177-4	2017	An in silico model of FAK/PI(4,5)P2 interaction suggests that residues on the catalytic domain interact with PI(4,5)P2, in addition to the known FERM domain PI(4,5)P2 binding site.	pi(4,5)p2	109-118	protein tyrosine kinase 2	Homo sapiens	22-25
28222177-6	2017	Mutations designed to disrupt the putative PI(4,5)P2 binding site were engineered into FAK.	pi(4,5)p2	43-52	protein tyrosine kinase 2	Homo sapiens	87-90
28222177-8	2017	The catalytic domain of FAK exhibited PI(4,5)P2 binding in vitro and binding activity was lost upon mutation of putative PI(4,5)P2 binding site basic residues.	pi(4,5)p2	38-47	protein tyrosine kinase 2	Homo sapiens	24-27
28222177-8	2017	The catalytic domain of FAK exhibited PI(4,5)P2 binding in vitro and binding activity was lost upon mutation of putative PI(4,5)P2 binding site basic residues.	pi(4,5)p2	121-130	protein tyrosine kinase 2	Homo sapiens	24-27
28209843-4	2017	A lipid-binding module in TMEM24 transports the phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] precursor phosphatidylinositol between bilayers, allowing replenishment of PI(4,5)P2 hydrolyzed during signaling.	pi(4,5)p2	87-96	C2CD2 like	Homo sapiens	26-32
28231468-1	2017	Synaptojanin 1 (SJ1) is a major presynaptic phosphatase that couples synaptic vesicle endocytosis to the dephosphorylation of PI(4,5)P2, a reaction needed for the shedding of endocytic factors from their membranes.	pi(4,5)p2	126-135	synaptojanin 1	Homo sapiens	0-14
28231468-1	2017	Synaptojanin 1 (SJ1) is a major presynaptic phosphatase that couples synaptic vesicle endocytosis to the dephosphorylation of PI(4,5)P2, a reaction needed for the shedding of endocytic factors from their membranes.	pi(4,5)p2	126-135	synaptojanin 1	Homo sapiens	16-19
28209843-4	2017	A lipid-binding module in TMEM24 transports the phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] precursor phosphatidylinositol between bilayers, allowing replenishment of PI(4,5)P2 hydrolyzed during signaling.	pi(4,5)p2	173-182	C2CD2 like	Homo sapiens	26-32
28143933-8	2017	A combination of MYO6+ mutagenesis and siRNA-mediated depletion of MYO6 binding partners demonstrates that motor activity and binding to endosomal membranes mediated by GIPC and PI(4,5)P2 are crucial for filopodia formation.	pi(4,5)p2	178-187	myosin VI	Homo sapiens	17-21
28143933-8	2017	A combination of MYO6+ mutagenesis and siRNA-mediated depletion of MYO6 binding partners demonstrates that motor activity and binding to endosomal membranes mediated by GIPC and PI(4,5)P2 are crucial for filopodia formation.	pi(4,5)p2	178-187	myosin VI	Homo sapiens	67-71
28035047-5	2017	LYCAT silencing selectively perturbed the levels and localization of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] and phosphatidylinositol-3-phosphate and the membrane traffic dependent on these specific PIPs but was without effect on phosphatidylinositol-4-phosphate or biosynthetic membrane traffic.	pi(4,5)p2	108-117	lysocardiolipin acyltransferase 1	Homo sapiens	0-5
27998989-6	2017	INPP5E"s phosphoinositide substrates PI(4,5)P2 and PI(3,4,5)P3 accumulated at the transition zone (TZ) in Hedgehog-stimulated Inpp5e-/- cells, which was associated with reduced recruitment of TZ scaffolding proteins and reduced Smoothened levels at cilia.	pi(4,5)p2	37-46	inositol polyphosphate-5-phosphatase E	Mus musculus	0-6
27998989-6	2017	INPP5E"s phosphoinositide substrates PI(4,5)P2 and PI(3,4,5)P3 accumulated at the transition zone (TZ) in Hedgehog-stimulated Inpp5e-/- cells, which was associated with reduced recruitment of TZ scaffolding proteins and reduced Smoothened levels at cilia.	pi(4,5)p2	37-46	inositol polyphosphate-5-phosphatase E	Mus musculus	126-132
28063499-3	2017	It is of interest that PI(4,5)P2 is also a critical cofactor for the activation of the PA-generating enzyme, phospholipase D (PLD).	pi(4,5)p2	23-32	glycosylphosphatidylinositol specific phospholipase D1	Homo sapiens	109-124
28063499-3	2017	It is of interest that PI(4,5)P2 is also a critical cofactor for the activation of the PA-generating enzyme, phospholipase D (PLD).	pi(4,5)p2	23-32	glycosylphosphatidylinositol specific phospholipase D1	Homo sapiens	126-129
28035047-6	2017	The acyl profile of PI(4,5)P2 was selectively altered in LYCAT-deficient cells, whereas LYCAT localized with phosphatidylinositol synthase.	pi(4,5)p2	20-29	lysocardiolipin acyltransferase 1	Homo sapiens	57-62
28035047-7	2017	We propose that LYCAT remodels the acyl chains of PI, which is then channeled into PI(4,5)P2 Our observations suggest that the PIP acyl chain profile may exert broad control of cell physiology.	pi(4,5)p2	83-92	lysocardiolipin acyltransferase 1	Homo sapiens	16-21
28035047-7	2017	We propose that LYCAT remodels the acyl chains of PI, which is then channeled into PI(4,5)P2 Our observations suggest that the PIP acyl chain profile may exert broad control of cell physiology.	pi(4,5)p2	83-92	prolactin induced protein	Homo sapiens	127-130
29020859-1	2017	Abstract: Phosphatidylinositol 4-phosphate 5-kinase type 1 gamma (Pip5k1c) generates phosphatidylinositol 4,5-bisphosphate, alsoknown as PI(4,5)P2 or PIP2.	pi(4,5)p2	137-146	phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma	Mus musculus	10-64
29020859-1	2017	Abstract: Phosphatidylinositol 4-phosphate 5-kinase type 1 gamma (Pip5k1c) generates phosphatidylinositol 4,5-bisphosphate, alsoknown as PI(4,5)P2 or PIP2.	pi(4,5)p2	137-146	phosphatidylinositol-4-phosphate 5-kinase, type 1 gamma	Mus musculus	66-73
27418354-3	2016	Here, we show that stimulation of murine peritoneal macrophages with LPS induces biphasic accumulation of PI(4,5)P2 with peaks at 10 and 60-90 min that were still seen after silencing of TLR4 expression.	pi(4,5)p2	106-115	toll-like receptor 4	Mus musculus	187-191
28008947-0	2016	Self assembly of HIV-1 Gag protein on lipid membranes generates PI(4,5)P2/Cholesterol nanoclusters.	pi(4,5)p2	64-73	Pr55(Gag)	Human immunodeficiency virus 1	23-26
27418354-4	2016	In contrast, the PI(4,5)P2 elevation was abrogated when CD14 was removed from the cell surface.	pi(4,5)p2	17-26	CD14 antigen	Mus musculus	56-60
27881774-5	2016	An in vitro assay showed that the region I, region II, and region I+II (D51/E52/E55/D166A) mutants markedly reduced the attachment between syt-1 and t-SNARE-carrying vesicles in the absence of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	232-241	synaptotagmin I	Mus musculus	139-144
27881774-5	2016	An in vitro assay showed that the region I, region II, and region I+II (D51/E52/E55/D166A) mutants markedly reduced the attachment between syt-1 and t-SNARE-carrying vesicles in the absence of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	232-241	vesicle transport through interaction with t-SNAREs 1B	Mus musculus	151-156
28138522-1	2016	Phosphatidylinositol 4-phosphate 5-kinase (PIP5K), a representative member of the phosphatidylinositol phosphate kinase (PIPK) family, is a major enzyme that biosynthesizes the signaling molecule PI(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) in eukaryotic cells.	pi(4,5)p2	196-205	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	0-41
28138522-1	2016	Phosphatidylinositol 4-phosphate 5-kinase (PIP5K), a representative member of the phosphatidylinositol phosphate kinase (PIPK) family, is a major enzyme that biosynthesizes the signaling molecule PI(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) in eukaryotic cells.	pi(4,5)p2	196-205	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	43-48
27650500-3	2016	We report that an evolutionarily conserved cluster of histidine residues located in the C-terminal cytoplasmic domain between two phosphatidylinositol 4,5-bisphosphate binding sites (PI(4,5)P2) of NHE1 confers pH-dependent PI(4,5)P2 binding and regulates NHE1 activity.	pi(4,5)p2	183-192	solute carrier family 9 member A1	Homo sapiens	197-201
27418354-7	2016	With an increasing amount of CD14 and concomitant decrease of TLR4, 2 peaks of PI(4,5)P2 accumulation appeared, eventually approaching those found in LPS-stimulated cells expressing CD14 alone.	pi(4,5)p2	79-88	CD14 antigen	Mus musculus	29-33
27418354-7	2016	With an increasing amount of CD14 and concomitant decrease of TLR4, 2 peaks of PI(4,5)P2 accumulation appeared, eventually approaching those found in LPS-stimulated cells expressing CD14 alone.	pi(4,5)p2	79-88	toll-like receptor 4	Mus musculus	62-66
27418354-7	2016	With an increasing amount of CD14 and concomitant decrease of TLR4, 2 peaks of PI(4,5)P2 accumulation appeared, eventually approaching those found in LPS-stimulated cells expressing CD14 alone.	pi(4,5)p2	79-88	CD14 antigen	Mus musculus	182-186
27418354-8	2016	Mutation of the signaling domain of TLR4 let us conclude that the receptor activity can modulate PI(4,5)P2 accumulation in cells when expressed in high amounts compared with CD14.	pi(4,5)p2	97-106	toll-like receptor 4	Mus musculus	36-40
27418354-10	2016	Inhibition of PLC and PI3K or overexpression of PI4K IIalpha that produces PI(4)P promoted PI(4,5)P2 elevation in LPS-stimulated cells.	pi(4,5)p2	91-100	perlecan (heparan sulfate proteoglycan 2)	Mus musculus	14-17
27418354-12	2016	Taken together, these data suggest that LPS-induced accumulation of PI(4,5)P2 that maximizes TLR4 signaling is controlled by CD14, whereas TLR4 can fine tune the process by affecting the PI(4,5)P2 turnover.	pi(4,5)p2	68-77	toll-like receptor 4	Mus musculus	93-97
27418354-12	2016	Taken together, these data suggest that LPS-induced accumulation of PI(4,5)P2 that maximizes TLR4 signaling is controlled by CD14, whereas TLR4 can fine tune the process by affecting the PI(4,5)P2 turnover.	pi(4,5)p2	68-77	CD14 antigen	Mus musculus	125-129
27418354-12	2016	Taken together, these data suggest that LPS-induced accumulation of PI(4,5)P2 that maximizes TLR4 signaling is controlled by CD14, whereas TLR4 can fine tune the process by affecting the PI(4,5)P2 turnover.	pi(4,5)p2	187-196	toll-like receptor 4	Mus musculus	139-143
27578150-5	2016	The application of these two complementary approaches helped us to reveal that matrix protein specifically recognizes the PI(4,5)P2 molecule by the residues K20, K25, K27, K74, and Y28, while the residues K92 and K93 stabilizes the matrix protein orientation on the membrane by the interaction with another PI(4,5)P2 molecule.	pi(4,5)p2	122-131	keratin 20	Homo sapiens	157-160
27578150-5	2016	The application of these two complementary approaches helped us to reveal that matrix protein specifically recognizes the PI(4,5)P2 molecule by the residues K20, K25, K27, K74, and Y28, while the residues K92 and K93 stabilizes the matrix protein orientation on the membrane by the interaction with another PI(4,5)P2 molecule.	pi(4,5)p2	122-131	keratin 25	Homo sapiens	162-165
27578150-5	2016	The application of these two complementary approaches helped us to reveal that matrix protein specifically recognizes the PI(4,5)P2 molecule by the residues K20, K25, K27, K74, and Y28, while the residues K92 and K93 stabilizes the matrix protein orientation on the membrane by the interaction with another PI(4,5)P2 molecule.	pi(4,5)p2	122-131	keratin 27	Homo sapiens	167-170
27578150-5	2016	The application of these two complementary approaches helped us to reveal that matrix protein specifically recognizes the PI(4,5)P2 molecule by the residues K20, K25, K27, K74, and Y28, while the residues K92 and K93 stabilizes the matrix protein orientation on the membrane by the interaction with another PI(4,5)P2 molecule.	pi(4,5)p2	122-131	keratin 74	Homo sapiens	172-175
27650500-3	2016	We report that an evolutionarily conserved cluster of histidine residues located in the C-terminal cytoplasmic domain between two phosphatidylinositol 4,5-bisphosphate binding sites (PI(4,5)P2) of NHE1 confers pH-dependent PI(4,5)P2 binding and regulates NHE1 activity.	pi(4,5)p2	183-192	solute carrier family 9 member A1	Homo sapiens	255-259
27650500-3	2016	We report that an evolutionarily conserved cluster of histidine residues located in the C-terminal cytoplasmic domain between two phosphatidylinositol 4,5-bisphosphate binding sites (PI(4,5)P2) of NHE1 confers pH-dependent PI(4,5)P2 binding and regulates NHE1 activity.	pi(4,5)p2	223-232	solute carrier family 9 member A1	Homo sapiens	197-201
27650500-3	2016	We report that an evolutionarily conserved cluster of histidine residues located in the C-terminal cytoplasmic domain between two phosphatidylinositol 4,5-bisphosphate binding sites (PI(4,5)P2) of NHE1 confers pH-dependent PI(4,5)P2 binding and regulates NHE1 activity.	pi(4,5)p2	223-232	solute carrier family 9 member A1	Homo sapiens	255-259
27650500-4	2016	A GST fusion of the wild type C-terminal cytoplasmic domain of NHE1 showed increased maximum PI(4,5)P2 binding at pH 7.0 compared with pH 7.5.	pi(4,5)p2	93-102	solute carrier family 9 member A1	Homo sapiens	63-67
27650500-8	2016	These data identify a molecular mechanism for pH-sensitive PI(4,5)P2 binding regulating NHE1 activity and suggest that the evolutionarily conserved cluster of four histidines in the proximal cytoplasmic domain of NHE1 may constitute a proton modifier site.	pi(4,5)p2	59-68	solute carrier family 9 member A1	Homo sapiens	88-92
27487483-4	2016	Our results show that two palladin domains [immunoglobulin (Ig) 3 and 34] interact with the head group of PI(4,5)P2 with moderate affinity (apparent Kd=17muM).	pi(4,5)p2	106-115	palladin, cytoskeletal associated protein	Homo sapiens	26-34
27487483-8	2016	Our results provide biochemical proof that PI(4,5)P2 functions as a moderator of palladin activity and have also identified residues directly involved in the crosslinking activity of palladin.	pi(4,5)p2	43-52	palladin, cytoskeletal associated protein	Homo sapiens	81-89
27487483-8	2016	Our results provide biochemical proof that PI(4,5)P2 functions as a moderator of palladin activity and have also identified residues directly involved in the crosslinking activity of palladin.	pi(4,5)p2	43-52	palladin, cytoskeletal associated protein	Homo sapiens	183-191
27601656-0	2016	In B cells, phosphatidylinositol 5-phosphate 4-kinase-alpha synthesizes PI(4,5)P2 to impact mTORC2 and Akt signaling.	pi(4,5)p2	72-81	CREB regulated transcription coactivator 2	Mus musculus	92-98
27650500-8	2016	These data identify a molecular mechanism for pH-sensitive PI(4,5)P2 binding regulating NHE1 activity and suggest that the evolutionarily conserved cluster of four histidines in the proximal cytoplasmic domain of NHE1 may constitute a proton modifier site.	pi(4,5)p2	59-68	solute carrier family 9 member A1	Homo sapiens	213-217
27706148-6	2016	HomoFRET between full-length PKCalpha molecules is observed when in solution with both calcium and liposomes containing either diacylglycerol (DAG) or phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	190-199	protein kinase C alpha	Homo sapiens	29-37
27601656-0	2016	In B cells, phosphatidylinositol 5-phosphate 4-kinase-alpha synthesizes PI(4,5)P2 to impact mTORC2 and Akt signaling.	pi(4,5)p2	72-81	AKT serine/threonine kinase 1	Homo sapiens	103-106
27440886-3	2016	The binding and localization of HIV-1 Gag to the PM are mediated by the interaction between the matrix (MA) domain, specifically the highly basic region, and a PM-specific acidic phospholipid, phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	232-241	Pr55(Gag)	Human immunodeficiency virus 1	38-41
27291418-4	2016	Here we report that two members of the Transient Receptor Potential Vanilloid (TRPV) ion channel family, TRPV5 and TRPV6 lack a positively charged residue in the TM4-TM5 loop that was shown to interact with PI(4,5)P2 in TRPV1, which shows high affinity for this lipid.	pi(4,5)p2	207-216	transient receptor potential cation channel subfamily V member 5	Homo sapiens	105-110
27528604-3	2016	CAPS binds phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and SNARE proteins and is proposed to promote SNARE protein complex assembly for vesicle docking and priming.	pi(4,5)p2	50-59	calcium dependent secretion activator	Rattus norvegicus	0-4
27528604-5	2016	However, the active form of CAPS bound to PC12 cell membranes or to liposomes containing PI(4,5)P2 and Q-SNARE proteins was mainly dimer.	pi(4,5)p2	89-98	calcium dependent secretion activator	Rattus norvegicus	28-32
27283411-0	2016	Inhibition of TREK-2 K(+) channels by PI(4,5)P2: an intrinsic mode of regulation by intracellular ATP via phosphatidylinositol kinase.	pi(4,5)p2	38-47	potassium channel, subfamily K, member 10	Mus musculus	14-20
27283411-12	2016	The inhibition of TREK-2 by unspecific electrostatic quenching, extensive dephosphorylation, or sustained hydrolysis of PI(4,5)P2 suggests the existence of dual regulatory modes that depend on PI(4,5)P2 concentration.	pi(4,5)p2	120-129	potassium channel, subfamily K, member 10	Mus musculus	18-24
27283411-12	2016	The inhibition of TREK-2 by unspecific electrostatic quenching, extensive dephosphorylation, or sustained hydrolysis of PI(4,5)P2 suggests the existence of dual regulatory modes that depend on PI(4,5)P2 concentration.	pi(4,5)p2	193-202	potassium channel, subfamily K, member 10	Mus musculus	18-24
27819060-1	2016	Phosphoinositide 3-kinase (PI3K) generation of PI(3,4,5)P3 from PI(4,5)P2 and the subsequent activation of Akt and its downstream signaling cascades (e.g. mTORC1) dominates the landscape of phosphoinositide signaling axis in cancer research.	pi(4,5)p2	64-73	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta	Homo sapiens	0-25
27819060-1	2016	Phosphoinositide 3-kinase (PI3K) generation of PI(3,4,5)P3 from PI(4,5)P2 and the subsequent activation of Akt and its downstream signaling cascades (e.g. mTORC1) dominates the landscape of phosphoinositide signaling axis in cancer research.	pi(4,5)p2	64-73	AKT serine/threonine kinase 1	Homo sapiens	107-110
27819060-1	2016	Phosphoinositide 3-kinase (PI3K) generation of PI(3,4,5)P3 from PI(4,5)P2 and the subsequent activation of Akt and its downstream signaling cascades (e.g. mTORC1) dominates the landscape of phosphoinositide signaling axis in cancer research.	pi(4,5)p2	64-73	CREB regulated transcription coactivator 1	Mus musculus	155-161
27222577-9	2016	For zebrafish VSP, our modeling revealed that 3-phosphatase activity against PI(3,4,5)P3 is 55-fold slower than 5-phosphatase activity against PI(4,5)P2; thus, PI(4,5)P2 generated more slowly from dephosphorylating PI(3,4,5)P3 might never accumulate.	pi(4,5)p2	143-152	transmembrane phosphatase with tensin homology	Danio rerio	14-17
27222577-9	2016	For zebrafish VSP, our modeling revealed that 3-phosphatase activity against PI(3,4,5)P3 is 55-fold slower than 5-phosphatase activity against PI(4,5)P2; thus, PI(4,5)P2 generated more slowly from dephosphorylating PI(3,4,5)P3 might never accumulate.	pi(4,5)p2	160-169	transmembrane phosphatase with tensin homology	Danio rerio	14-17
27217553-0	2016	Osmoregulatory inositol transporter SMIT1 modulates electrical activity by adjusting PI(4,5)P2 levels.	pi(4,5)p2	85-94	solute carrier family 5 member 3	Homo sapiens	36-41
27217553-5	2016	We found that overexpression of the Na(+)/myo-inositol cotransporter (SMIT1) and myo-inositol supplementation enlarged intracellular PI(4,5)P2 pools, modulated several PI(4,5)P2-dependent ion channels including KCNQ2/3 channels, and attenuated the action potential firing of superior cervical ganglion neurons.	pi(4,5)p2	133-142	solute carrier family 5 member 3	Homo sapiens	70-75
27217553-5	2016	We found that overexpression of the Na(+)/myo-inositol cotransporter (SMIT1) and myo-inositol supplementation enlarged intracellular PI(4,5)P2 pools, modulated several PI(4,5)P2-dependent ion channels including KCNQ2/3 channels, and attenuated the action potential firing of superior cervical ganglion neurons.	pi(4,5)p2	168-177	solute carrier family 5 member 3	Homo sapiens	70-75
27272733-2	2016	Here we present evidence that EHBP-1 associates with tubular endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] enriched membranes through an N-terminal C2-like (NT-C2) domain, and define residues within the NT-C2 domain that mediate membrane interaction.	pi(4,5)p2	110-119	EH (Eps-15-homology) domain Binding Protein family	Caenorhabditis elegans	30-36
27291418-4	2016	Here we report that two members of the Transient Receptor Potential Vanilloid (TRPV) ion channel family, TRPV5 and TRPV6 lack a positively charged residue in the TM4-TM5 loop that was shown to interact with PI(4,5)P2 in TRPV1, which shows high affinity for this lipid.	pi(4,5)p2	207-216	transient receptor potential cation channel subfamily V member 6	Homo sapiens	115-120
27291418-4	2016	Here we report that two members of the Transient Receptor Potential Vanilloid (TRPV) ion channel family, TRPV5 and TRPV6 lack a positively charged residue in the TM4-TM5 loop that was shown to interact with PI(4,5)P2 in TRPV1, which shows high affinity for this lipid.	pi(4,5)p2	207-216	transient receptor potential cation channel subfamily V member 1	Homo sapiens	220-225
27291418-5	2016	When this positively charged residue was introduced to either TRPV6 or TRPV5, they displayed markedly higher affinities for PI(4,5)P2, and were largely resistant to inhibition by PI(4,5)P2 depletion.	pi(4,5)p2	124-133	transient receptor potential cation channel subfamily V member 6	Homo sapiens	62-67
27291418-5	2016	When this positively charged residue was introduced to either TRPV6 or TRPV5, they displayed markedly higher affinities for PI(4,5)P2, and were largely resistant to inhibition by PI(4,5)P2 depletion.	pi(4,5)p2	124-133	transient receptor potential cation channel subfamily V member 5	Homo sapiens	71-76
27291418-5	2016	When this positively charged residue was introduced to either TRPV6 or TRPV5, they displayed markedly higher affinities for PI(4,5)P2, and were largely resistant to inhibition by PI(4,5)P2 depletion.	pi(4,5)p2	179-188	transient receptor potential cation channel subfamily V member 6	Homo sapiens	62-67
27291418-5	2016	When this positively charged residue was introduced to either TRPV6 or TRPV5, they displayed markedly higher affinities for PI(4,5)P2, and were largely resistant to inhibition by PI(4,5)P2 depletion.	pi(4,5)p2	179-188	transient receptor potential cation channel subfamily V member 5	Homo sapiens	71-76
27291418-6	2016	Furthermore, Ca(2+)-induced inactivation of TRPV6 was essentially eliminated in the G488R mutant, showing the importance of PLC-mediated PI(4,5)P2 depletion in this process.	pi(4,5)p2	137-146	transient receptor potential cation channel subfamily V member 6	Homo sapiens	44-49
26212011-4	2016	We show that the decrease in PI(4,5)P2 level under non-stimulated conditions inhibits PTEN activity leading to the aberrant activation of the oncoprotein Akt.	pi(4,5)p2	29-38	phosphatase and tensin homolog	Homo sapiens	86-90
27118725-2	2016	One-step treatment with PI 4-kinase IIIbeta (PI4Kbeta) yielded PI 4-phosphate (PI4P), while a multistep enzymatic cascade of PI4Kbeta followed by PIP 5-kinase produced PI-4,5-bisphosphate (PI(4,5)P2 or PIP2).	pi(4,5)p2	189-198	phosphatidylinositol 4-kinase beta	Homo sapiens	45-53
27118725-2	2016	One-step treatment with PI 4-kinase IIIbeta (PI4Kbeta) yielded PI 4-phosphate (PI4P), while a multistep enzymatic cascade of PI4Kbeta followed by PIP 5-kinase produced PI-4,5-bisphosphate (PI(4,5)P2 or PIP2).	pi(4,5)p2	189-198	phosphatidylinositol 4-kinase beta	Homo sapiens	125-133
26775587-8	2016	The small GTPase ADP-ribosylation factor 1 (Arf1), for example, is known to stimulate synthesis of PI4P and PI(4,5)P2 on the Golgi to regulate protein and lipid sorting.	pi(4,5)p2	108-117	ADP ribosylation factor 1	Homo sapiens	17-42
26775587-8	2016	The small GTPase ADP-ribosylation factor 1 (Arf1), for example, is known to stimulate synthesis of PI4P and PI(4,5)P2 on the Golgi to regulate protein and lipid sorting.	pi(4,5)p2	108-117	ADP ribosylation factor 1	Homo sapiens	44-48
26212011-4	2016	We show that the decrease in PI(4,5)P2 level under non-stimulated conditions inhibits PTEN activity leading to the aberrant activation of the oncoprotein Akt.	pi(4,5)p2	29-38	AKT serine/threonine kinase 1	Homo sapiens	154-157
26657293-0	2016	Human beta-defensin 3 contains an oncolytic motif that binds PI(4,5)P2 to mediate tumour cell permeabilisation.	pi(4,5)p2	61-70	defensin beta 103B	Homo sapiens	6-21
26923189-6	2016	We examined whether the depletion of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is responsible for the muscarinic suppression of CaV2.3 currents by using two methods: the Danio rerio voltage-sensing phosphatase (Dr-VSP) system and the rapamycin-induced translocatable pseudojanin (PJ) system.	pi(4,5)p2	76-85	caveolin 2	Danio rerio	136-140
26923189-7	2016	First, dephosphorylation of PI(4,5)P2 to phosphatidylinositol 4-phosphate (PI(4)P) by Dr-VSP significantly suppressed CaV2.3 currents, by 53 +- 3%.	pi(4,5)p2	28-37	transmembrane phosphatase with tensin homology	Danio rerio	86-92
26923189-7	2016	First, dephosphorylation of PI(4,5)P2 to phosphatidylinositol 4-phosphate (PI(4)P) by Dr-VSP significantly suppressed CaV2.3 currents, by 53 +- 3%.	pi(4,5)p2	28-37	caveolin 2	Danio rerio	118-122
26923189-9	2016	The results suggest that CaV2.3 currents are modulated by the M1 receptor in a dual mode-that is, potentiation through the activation of PKC and suppression by the depletion of membrane PI(4,5)P2.	pi(4,5)p2	186-195	caveolin 2	Danio rerio	25-29
26826186-0	2016	SHIP2 controls plasma membrane PI(4,5)P2 thereby participating in the control of cell migration in 1321 N1 glioblastoma cells.	pi(4,5)p2	31-40	inositol polyphosphate phosphatase like 1	Homo sapiens	0-5
26826186-3	2016	Although the preferred SHIP2 substrate is PI(3,4,5)P3, PI(4,5)P2 can also be dephosphorylated by this enzyme to phosphatidylinositol 4-phosphate (PI4P).	pi(4,5)p2	55-64	inositol polyphosphate phosphatase like 1	Homo sapiens	23-28
26826186-5	2016	In different glioblastoma cell lines and primary cultures, SHIP2 staining at the plasma membrane partly overlaps with PI(4,5)P2 immunoreactivity.	pi(4,5)p2	118-127	inositol polyphosphate phosphatase like 1	Homo sapiens	59-64
26826186-7	2016	Therefore, SHIP2 controls both PI(3,4,5)P3 and PI(4,5)P2 levels in intact cells.	pi(4,5)p2	47-56	inositol polyphosphate phosphatase like 1	Homo sapiens	11-16
26826186-9	2016	Regulation of PI(4,5)P2 and PI4P content by SHIP2 controls 1321 N1 cell migration through the organization of focal adhesions.	pi(4,5)p2	14-23	inositol polyphosphate phosphatase like 1	Homo sapiens	44-49
26826186-10	2016	Thus, our results reveal a new role of SHIP2 in the control of PI(4,5)P2, PI4P and cell migration in PTEN-deficient glioblastoma 1321 N1 cells.	pi(4,5)p2	63-72	inositol polyphosphate phosphatase like 1	Homo sapiens	39-44
26724696-4	2016	Genetic and biochemical approaches showed that increased metabolism of PI(4,5)P2 reduces the activity of the Pho85 kinase by increasing the levels of the DPIP isomer 1-IP7.	pi(4,5)p2	71-80	cyclin-dependent serine/threonine-protein kinase PHO85	Saccharomyces cerevisiae S288C	109-114
26992353-6	2016	Native PI(4,5)P2 enhanced MA binding to liposomes designed to mimic non-raft-like regions of the membrane, suggesting the possibility that binding of the protein to disordered domains may precede Gag association with, or nucleation of, rafts.	pi(4,5)p2	7-16	Pr55(Gag)	Human immunodeficiency virus 1	196-199
26862217-0	2016	RdgBalpha reciprocally transfers PA and PI at ER-PM contact sites to maintain PI(4,5)P2 homoeostasis during phospholipase C signalling in Drosophila photoreceptors.	pi(4,5)p2	78-87	retinal degeneration B	Drosophila melanogaster	0-9
26862217-7	2016	We propose that RdgBalpha proteins maintain PI(4,5)P2 homoeostasis after PLC activation by facilitating the reciprocal transport of PA and PI at ER-PM membrane contact sites.	pi(4,5)p2	44-53	retinal degeneration B	Drosophila melanogaster	16-25
26657293-6	2016	The binding of HBD-3 to PI(4,5)P2 was shown to be critical for mediating cytolysis of tumour cells, suggesting a conserved mechanism of action for defensins across diverse species.	pi(4,5)p2	24-33	defensin beta 103B	Homo sapiens	15-20
26620550-0	2016	PTPRN2 and PLCbeta1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling.	pi(4,5)p2	76-85	protein tyrosine phosphatase receptor type N2	Homo sapiens	0-6
26476105-0	2016	n-3 polyunsaturated fatty acids suppress CD4(+) T cell proliferation by altering phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] organization.	pi(4,5)p2	122-131	CD4 antigen	Mus musculus	41-44
26476105-2	2016	We have previously demonstrated that n-3 PUFA decrease the amount of phosphatidylinositol-(4,5)-bisphosphate, [PI(4,5)P2], in CD4(+) T cells, leading to suppressed actin remodeling upon activation.	pi(4,5)p2	111-120	CD4 antigen	Mus musculus	126-129
26476105-5	2016	CD4(+) T cells enriched in n-3 PUFA also exhibited a depleted plasma membrane non-raft PI(4,5)P2 pool as detected by decreased co-clustering of Src(N15), a non-raft marker, and PH(PLC-delta), a PI(4,5)P2 reporter.	pi(4,5)p2	87-96	CD4 antigen	Mus musculus	0-3
26476105-6	2016	Incubation with exogenous PI(4,5)P2 rescued the effects on the non-raft PI(4,5)P2 pool, and reversed the suppression of T cell proliferation in CD4(+) T cells enriched with n-3 PUFA.	pi(4,5)p2	26-35	CD4 antigen	Mus musculus	144-147
26476105-7	2016	Furthermore, CD4(+) T cells isolated from mice fed a 4% docosahexaenoic acid (DHA)-enriched diet exhibited a decrease in the non-raft pool of PI(4,5)P2, and exogenous PI(4,5)P2 reversed the suppression of T cell proliferation.	pi(4,5)p2	142-151	CD4 antigen	Mus musculus	13-16
26476105-7	2016	Furthermore, CD4(+) T cells isolated from mice fed a 4% docosahexaenoic acid (DHA)-enriched diet exhibited a decrease in the non-raft pool of PI(4,5)P2, and exogenous PI(4,5)P2 reversed the suppression of T cell proliferation.	pi(4,5)p2	167-176	CD4 antigen	Mus musculus	13-16
26620550-0	2016	PTPRN2 and PLCbeta1 promote metastatic breast cancer cell migration through PI(4,5)P2-dependent actin remodeling.	pi(4,5)p2	76-85	phospholipase C beta 1	Homo sapiens	11-19
26620550-4	2016	We find that PTPRN2 and PLCbeta1 enzymatically reduce plasma membrane PI(4,5)P2 levels in metastatic breast cancer cells through two independent mechanisms.	pi(4,5)p2	70-79	protein tyrosine phosphatase receptor type N2	Homo sapiens	13-19
26620550-4	2016	We find that PTPRN2 and PLCbeta1 enzymatically reduce plasma membrane PI(4,5)P2 levels in metastatic breast cancer cells through two independent mechanisms.	pi(4,5)p2	70-79	phospholipase C beta 1	Homo sapiens	24-32
26620550-6	2016	Reduction in plasma membrane PI(4,5)P2 abundance by these enzymes releases the PI(4,5)P2-binding protein cofilin from its inactive membrane-associated state into the cytoplasm where it mediates actin turnover dynamics, thereby enhancing cellular migration and metastatic capacity.	pi(4,5)p2	29-38	cofilin 1	Homo sapiens	105-112
26620550-6	2016	Reduction in plasma membrane PI(4,5)P2 abundance by these enzymes releases the PI(4,5)P2-binding protein cofilin from its inactive membrane-associated state into the cytoplasm where it mediates actin turnover dynamics, thereby enhancing cellular migration and metastatic capacity.	pi(4,5)p2	79-88	cofilin 1	Homo sapiens	105-112
26471078-1	2015	Dephosphorylation of four major C-terminal tail sites and occupancy of the phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2]-binding site of PTEN cooperate to activate its phospholipid phosphatase activity and facilitate its recruitment to plasma membrane.	pi(4,5)p2	114-123	phosphatase and tensin homolog	Homo sapiens	141-145
26552690-2	2016	CDP-DAG is required for the synthesis of phosphatidylinositol; the parent molecule whence all seven phosphoinositides including the signaling molecules PI4P, PI(4,5)P2, and PI(3,4,5)P3 are derived.	pi(4,5)p2	158-167	cut like homeobox 1	Homo sapiens	0-3
26723017-2	2015	Inositol polyphosphate 5-phosphatase E (INPP5E) converts PI(4,5)P2 into PI4P and is required for proper ciliary function.	pi(4,5)p2	57-66	inositol polyphosphate-5-phosphatase E	Mus musculus	40-46
26723017-7	2015	The fact that Skittles expression phenocopies the dInpp5e mutants confirms a central role for PI(4,5)P2 in the regulation of dTULP, Inactive, and NOMPC localization.	pi(4,5)p2	94-103	king tubby	Drosophila melanogaster	125-130
26723017-7	2015	The fact that Skittles expression phenocopies the dInpp5e mutants confirms a central role for PI(4,5)P2 in the regulation of dTULP, Inactive, and NOMPC localization.	pi(4,5)p2	94-103	no mechanoreceptor potential C	Drosophila melanogaster	146-151
26416531-3	2015	Our prior cellular and structural studies demonstrated that the signaling phosphatidylinositols PI(4,5)P2 (PIP2) and PI(3,4,5)P3 (PIP3) bind and regulate SF-1 (Steroidogenic Factor-1, NR5A1), a close homolog of LRH-1.	pi(4,5)p2	96-105	nuclear receptor subfamily 5 group A member 1	Homo sapiens	154-158
26416531-3	2015	Our prior cellular and structural studies demonstrated that the signaling phosphatidylinositols PI(4,5)P2 (PIP2) and PI(3,4,5)P3 (PIP3) bind and regulate SF-1 (Steroidogenic Factor-1, NR5A1), a close homolog of LRH-1.	pi(4,5)p2	96-105	nuclear receptor subfamily 5 group A member 1	Homo sapiens	160-182
26416531-3	2015	Our prior cellular and structural studies demonstrated that the signaling phosphatidylinositols PI(4,5)P2 (PIP2) and PI(3,4,5)P3 (PIP3) bind and regulate SF-1 (Steroidogenic Factor-1, NR5A1), a close homolog of LRH-1.	pi(4,5)p2	96-105	nuclear receptor subfamily 5 group A member 1	Homo sapiens	184-189
26416531-3	2015	Our prior cellular and structural studies demonstrated that the signaling phosphatidylinositols PI(4,5)P2 (PIP2) and PI(3,4,5)P3 (PIP3) bind and regulate SF-1 (Steroidogenic Factor-1, NR5A1), a close homolog of LRH-1.	pi(4,5)p2	96-105	nuclear receptor subfamily 5 group A member 2	Homo sapiens	211-216
26432644-2	2015	Previously, we reported that Ca(2+)-dependent activation of phospholipase Ceta2 (PLCeta2) catalyzes PI(4,5)P2 hydrolysis, which affected vesicle exocytosis by regulating the activities of the lipid-dependent priming factors CAPS (also known as CADPS) and ubiquitous Munc13-2 in PC12 cells.	pi(4,5)p2	100-109	calcium dependent secretion activator	Rattus norvegicus	224-228
26432644-2	2015	Previously, we reported that Ca(2+)-dependent activation of phospholipase Ceta2 (PLCeta2) catalyzes PI(4,5)P2 hydrolysis, which affected vesicle exocytosis by regulating the activities of the lipid-dependent priming factors CAPS (also known as CADPS) and ubiquitous Munc13-2 in PC12 cells.	pi(4,5)p2	100-109	calcium dependent secretion activator	Rattus norvegicus	244-249
26432644-2	2015	Previously, we reported that Ca(2+)-dependent activation of phospholipase Ceta2 (PLCeta2) catalyzes PI(4,5)P2 hydrolysis, which affected vesicle exocytosis by regulating the activities of the lipid-dependent priming factors CAPS (also known as CADPS) and ubiquitous Munc13-2 in PC12 cells.	pi(4,5)p2	100-109	unc-13 homolog B	Rattus norvegicus	266-274
26471078-2	2015	Our investigation of the mechanism by which phosphorylation of these C-terminal sites controls the PI(4,5)P2-binding affinity and catalytic activity of PTEN resulted in the following findings.	pi(4,5)p2	99-108	phosphatase and tensin homolog	Homo sapiens	152-156
26471078-6	2015	Third, dephosphorylation of all four sites significantly decreases the affinity of PTEN for PI(4,5)P2.	pi(4,5)p2	92-101	phosphatase and tensin homolog	Homo sapiens	83-87
26471078-7	2015	Since PI(4,5)P2 is a major phospholipid co-localizing with the phospholipid- and phosphoprotein-substrates in plasma membrane, we hypothesise that the reduced affinity facilitates PTEN to "hop" on the plasma membrane to dephosphorylate these substrates.	pi(4,5)p2	6-15	phosphatase and tensin homolog	Homo sapiens	180-184
26446256-4	2015	After 5-10 min of LPS stimulation, CD14 underwent prominent clustering in the plasma membrane, accompanied by accumulation of PI(4,5)P2 and type-I phosphatidylinositol 4-phosphate 5-kinase (PIP5K) isoforms Ialpha and Igamma (encoded by Pip5k1a and Pip5k1c, respectively) in the CD14 region.	pi(4,5)p2	126-135	CD14 molecule	Homo sapiens	35-39
26446256-0	2015	LPS-induced clustering of CD14 triggers generation of PI(4,5)P2.	pi(4,5)p2	54-63	CD14 molecule	Homo sapiens	26-30
26682807-8	2015	The Mm-VSP phosphatase domain, fused to the VSD of a nonmammalian VSP, was also functional: activation resulted in PI(4,5)P2 depletion that was sufficient to inhibit the PI(4,5)P2-regulated KCNQ2/3 channels.	pi(4,5)p2	115-124	cathepsin G	Mus musculus	7-10
26682807-8	2015	The Mm-VSP phosphatase domain, fused to the VSD of a nonmammalian VSP, was also functional: activation resulted in PI(4,5)P2 depletion that was sufficient to inhibit the PI(4,5)P2-regulated KCNQ2/3 channels.	pi(4,5)p2	115-124	cathepsin G	Mus musculus	66-69
26682807-8	2015	The Mm-VSP phosphatase domain, fused to the VSD of a nonmammalian VSP, was also functional: activation resulted in PI(4,5)P2 depletion that was sufficient to inhibit the PI(4,5)P2-regulated KCNQ2/3 channels.	pi(4,5)p2	170-179	cathepsin G	Mus musculus	7-10
26682807-8	2015	The Mm-VSP phosphatase domain, fused to the VSD of a nonmammalian VSP, was also functional: activation resulted in PI(4,5)P2 depletion that was sufficient to inhibit the PI(4,5)P2-regulated KCNQ2/3 channels.	pi(4,5)p2	170-179	cathepsin G	Mus musculus	66-69
26682807-8	2015	The Mm-VSP phosphatase domain, fused to the VSD of a nonmammalian VSP, was also functional: activation resulted in PI(4,5)P2 depletion that was sufficient to inhibit the PI(4,5)P2-regulated KCNQ2/3 channels.	pi(4,5)p2	170-179	potassium voltage-gated channel subfamily Q member 2	Homo sapiens	190-195
26446256-2	2015	Here, we show that CD14 controls generation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] in response to LPS binding.	pi(4,5)p2	86-95	CD14 molecule	Homo sapiens	19-23
26446256-5	2015	Clustering of CD14 with antibodies, without LPS and TLR4 participation, was sufficient to trigger PI(4,5)P2 elevation.	pi(4,5)p2	98-107	CD14 molecule	Homo sapiens	14-18
26446256-3	2015	In J774 cells and HEK293 cells expressing CD14 exposed to 10-100 ng/ml LPS, the level of PI(4,5)P2 rose in a biphasic manner with peaks at 5-10 min and 60 min.	pi(4,5)p2	89-98	CD14 molecule	Homo sapiens	42-46
26446256-6	2015	The newly generated PI(4,5)P2 accumulated in rafts, which also accommodated CD14 and a large portion of PIP5K Ialpha and PIP5K Igamma.	pi(4,5)p2	20-29	CD14 molecule	Homo sapiens	76-80
26446256-6	2015	The newly generated PI(4,5)P2 accumulated in rafts, which also accommodated CD14 and a large portion of PIP5K Ialpha and PIP5K Igamma.	pi(4,5)p2	20-29	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	104-116
26446256-8	2015	Taken together, these data indicate that LPS induces clustering of CD14, which triggers PI(4,5)P2 generation in rafts that is required for maximal pro-inflammatory signaling of TLR4.	pi(4,5)p2	88-97	CD14 molecule	Homo sapiens	67-71
26446256-8	2015	Taken together, these data indicate that LPS induces clustering of CD14, which triggers PI(4,5)P2 generation in rafts that is required for maximal pro-inflammatory signaling of TLR4.	pi(4,5)p2	88-97	toll like receptor 4	Homo sapiens	177-181
26509711-10	2015	These results suggest that IRBIT forms signaling complexes with PIPKIalpha and NBCe1-B, whose activity is regulated by PI(4,5)P2.	pi(4,5)p2	119-128	S-adenosylhomocysteine hydrolase-like 1	Mus musculus	27-32
25843208-4	2015	This review discusses recent advances in understanding the molecular components that tether the ER and plasma membrane to form the ER/PM microdomains in which PI(4,5)P2 is enriched, and how dynamic targeting of the Orai1-STIM1 complex to PI(4,5)P2-poor and PI(4,5)P2-rich microdomains controls the activity of Orai1 and its regulation by Ca(2+) that is mediated by SARAF.	pi(4,5)p2	238-247	ORAI calcium release-activated calcium modulator 1	Homo sapiens	215-220
25843208-4	2015	This review discusses recent advances in understanding the molecular components that tether the ER and plasma membrane to form the ER/PM microdomains in which PI(4,5)P2 is enriched, and how dynamic targeting of the Orai1-STIM1 complex to PI(4,5)P2-poor and PI(4,5)P2-rich microdomains controls the activity of Orai1 and its regulation by Ca(2+) that is mediated by SARAF.	pi(4,5)p2	238-247	stromal interaction molecule 1	Homo sapiens	221-226
25843208-4	2015	This review discusses recent advances in understanding the molecular components that tether the ER and plasma membrane to form the ER/PM microdomains in which PI(4,5)P2 is enriched, and how dynamic targeting of the Orai1-STIM1 complex to PI(4,5)P2-poor and PI(4,5)P2-rich microdomains controls the activity of Orai1 and its regulation by Ca(2+) that is mediated by SARAF.	pi(4,5)p2	238-247	ORAI calcium release-activated calcium modulator 1	Homo sapiens	215-220
25843208-4	2015	This review discusses recent advances in understanding the molecular components that tether the ER and plasma membrane to form the ER/PM microdomains in which PI(4,5)P2 is enriched, and how dynamic targeting of the Orai1-STIM1 complex to PI(4,5)P2-poor and PI(4,5)P2-rich microdomains controls the activity of Orai1 and its regulation by Ca(2+) that is mediated by SARAF.	pi(4,5)p2	238-247	stromal interaction molecule 1	Homo sapiens	221-226
25754030-4	2015	This review summarizes the literature on this topic and provides a detailed and critical discussion on the experimental evidence for the various effects of phosphatidylinositol 4,5-bisphosphayte [PI(4,5)P2 or PIP2] on TRPV1.	pi(4,5)p2	196-205	transient receptor potential cation channel subfamily V member 1	Homo sapiens	218-223
26183781-1	2015	HIV-Tat has been demonstrated to be secreted from cells in a phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2)-dependent manner.	pi(4,5)p2	100-109	tyrosine aminotransferase	Homo sapiens	4-7
26183781-4	2015	Although membrane binding of HIV-Tat did not strictly depend on PI(4,5)P2 but, rather, was mediated by a range of acidic membrane lipids, a functional interaction between PI(4,5)P2 and HIV-Tat was critically required for efficient membrane pore formation by HIV-Tat oligomers.	pi(4,5)p2	64-73	tyrosine aminotransferase	Homo sapiens	33-36
26183781-4	2015	Although membrane binding of HIV-Tat did not strictly depend on PI(4,5)P2 but, rather, was mediated by a range of acidic membrane lipids, a functional interaction between PI(4,5)P2 and HIV-Tat was critically required for efficient membrane pore formation by HIV-Tat oligomers.	pi(4,5)p2	171-180	tyrosine aminotransferase	Homo sapiens	33-36
26183781-4	2015	Although membrane binding of HIV-Tat did not strictly depend on PI(4,5)P2 but, rather, was mediated by a range of acidic membrane lipids, a functional interaction between PI(4,5)P2 and HIV-Tat was critically required for efficient membrane pore formation by HIV-Tat oligomers.	pi(4,5)p2	171-180	tyrosine aminotransferase	Homo sapiens	189-192
26183781-4	2015	Although membrane binding of HIV-Tat did not strictly depend on PI(4,5)P2 but, rather, was mediated by a range of acidic membrane lipids, a functional interaction between PI(4,5)P2 and HIV-Tat was critically required for efficient membrane pore formation by HIV-Tat oligomers.	pi(4,5)p2	171-180	tyrosine aminotransferase	Homo sapiens	189-192
26202220-3	2015	By exposing semi-intact cells to buffers of variable Ca(2+) concentrations, we found that binding of E-Syt1 to the PI(4,5)P2-rich PM critically requires its C2C and C2E domains and that the EC50 of such binding is in the low micromolar Ca(2+) range.	pi(4,5)p2	115-124	extended synaptotagmin 1	Homo sapiens	101-107
25754030-5	2015	We conclude that PI(4,5)P2 and potentially its precursor PI(4)P are positive cofactors for TRPV1, acting via direct interaction with the channel, and their depletion by Ca(2+)-induced activation of phospholipase Cdelta isoforms (PLCdelta) limits channel activity during capsaicin-induced desensitization.	pi(4,5)p2	17-26	transient receptor potential cation channel subfamily V member 1	Homo sapiens	91-96
26312755-6	2015	Here we identify key residues involved both in Ysc84 actin and lipid binding, and demonstrate that its actin binding activity is negatively regulated by PI(4,5)P2.	pi(4,5)p2	153-162	Ysc84p	Saccharomyces cerevisiae S288C	47-52
26312755-6	2015	Here we identify key residues involved both in Ysc84 actin and lipid binding, and demonstrate that its actin binding activity is negatively regulated by PI(4,5)P2.	pi(4,5)p2	153-162	actin	Saccharomyces cerevisiae S288C	103-108
26305592-8	2015	Increasing ciliary PI(4,5)P2 levels or conferring the ability to bind PI(4)P on Tulp3 increases the ciliary localization of Tulp3.	pi(4,5)p2	19-28	TUB like protein 3	Homo sapiens	124-129
26190144-4	2015	Upon INPP5E inactivation, PI(4,5)P2 accumulates at the ciliary tip whereas PI4P is depleted.	pi(4,5)p2	26-35	inositol polyphosphate-5-phosphatase E	Homo sapiens	5-11
25280637-4	2015	PI(4,5)P2directly regulates vesicle exocytosis by recruiting and activating PI(4,5)P2-binding proteins that regulate SNARE protein function including CAPS, Munc13-1/2, synaptotagmin-1, and other C2 domain-containing proteins.	pi(4,5)p2	0-9	small NF90 (ILF3) associated RNA E	Homo sapiens	117-122
25995263-1	2015	UNLABELLED: HIV-1 Gag, which drives virion assembly, interacts with a plasma membrane (PM)-specific phosphoinositide, phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2].	pi(4,5)p2	159-168	Pr55(Gag)	Human immunodeficiency virus 1	18-21
25995263-2	2015	While cellular acidic phospholipid-binding proteins/domains, such as the PI(4,5)P2-specific pleckstrin homology domain of phospholipase Cdelta1 (PHPLCdelta1), mediate headgroup-specific interactions with corresponding phospholipids, the exact nature of the Gag-PI(4,5)P2 interaction remains undetermined.	pi(4,5)p2	73-82	phospholipase C delta 1	Homo sapiens	122-156
25995263-2	2015	While cellular acidic phospholipid-binding proteins/domains, such as the PI(4,5)P2-specific pleckstrin homology domain of phospholipase Cdelta1 (PHPLCdelta1), mediate headgroup-specific interactions with corresponding phospholipids, the exact nature of the Gag-PI(4,5)P2 interaction remains undetermined.	pi(4,5)p2	73-82	Pr55(Gag)	Human immunodeficiency virus 1	257-260
25995263-3	2015	In this study, we used giant unilamellar vesicles (GUVs) to examine how PI(4,5)P2 with unsaturated or saturated acyl chains affect membrane binding of PHPLCdelta1 and Gag.	pi(4,5)p2	72-81	Pr55(Gag)	Human immunodeficiency virus 1	167-170
25995263-8	2015	Likewise, Gag bound to the liquid phase when PI(4,5)P2 had DO-acyl chains.	pi(4,5)p2	45-54	Pr55(Gag)	Human immunodeficiency virus 1	10-13
25995263-11	2015	Altogether, these results revealed different roles for PI(4,5)P2 acyl chains in membrane binding of two PI(4,5)P2-binding proteins, Gag and PHPLCdelta1.	pi(4,5)p2	55-64	Pr55(Gag)	Human immunodeficiency virus 1	132-135
25995263-11	2015	Altogether, these results revealed different roles for PI(4,5)P2 acyl chains in membrane binding of two PI(4,5)P2-binding proteins, Gag and PHPLCdelta1.	pi(4,5)p2	104-113	Pr55(Gag)	Human immunodeficiency virus 1	132-135
25995263-12	2015	Notably, we observed that nonmyristylated Gag retains the preference for PI(4,5)P2 containing an unsaturated acyl chain over DP-PI(4,5)P2, suggesting that Gag sensitivity to PI(4,5)P2 acyl chain saturation is determined directly by the matrix-PI(4,5)P2 interaction, rather than indirectly by a myristate-dependent mechanism.	pi(4,5)p2	73-82	Pr55(Gag)	Human immunodeficiency virus 1	42-45
25995263-12	2015	Notably, we observed that nonmyristylated Gag retains the preference for PI(4,5)P2 containing an unsaturated acyl chain over DP-PI(4,5)P2, suggesting that Gag sensitivity to PI(4,5)P2 acyl chain saturation is determined directly by the matrix-PI(4,5)P2 interaction, rather than indirectly by a myristate-dependent mechanism.	pi(4,5)p2	73-82	Pr55(Gag)	Human immunodeficiency virus 1	155-158
25995263-12	2015	Notably, we observed that nonmyristylated Gag retains the preference for PI(4,5)P2 containing an unsaturated acyl chain over DP-PI(4,5)P2, suggesting that Gag sensitivity to PI(4,5)P2 acyl chain saturation is determined directly by the matrix-PI(4,5)P2 interaction, rather than indirectly by a myristate-dependent mechanism.	pi(4,5)p2	128-137	Pr55(Gag)	Human immunodeficiency virus 1	42-45
25995263-12	2015	Notably, we observed that nonmyristylated Gag retains the preference for PI(4,5)P2 containing an unsaturated acyl chain over DP-PI(4,5)P2, suggesting that Gag sensitivity to PI(4,5)P2 acyl chain saturation is determined directly by the matrix-PI(4,5)P2 interaction, rather than indirectly by a myristate-dependent mechanism.	pi(4,5)p2	128-137	Pr55(Gag)	Human immunodeficiency virus 1	155-158
25995263-13	2015	IMPORTANCE: Binding of HIV-1 Gag to the plasma membrane is promoted by its interaction with a plasma membrane-localized phospholipid, PI(4,5)P2.	pi(4,5)p2	134-143	Pr55(Gag)	Human immunodeficiency virus 1	29-32
25995263-16	2015	Using a novel giant unilamellar vesicle-based system, we found that PI(4,5)P2 with an unsaturated acyl chain recruited PHPLCdelta1 and Gag similarly, whereas PI(4,5)P2 with saturated acyl chains either recruited PHPLCdelta1 but not Gag or sorted these proteins to different phases of vesicles.	pi(4,5)p2	68-77	Pr55(Gag)	Human immunodeficiency virus 1	135-138
25995263-16	2015	Using a novel giant unilamellar vesicle-based system, we found that PI(4,5)P2 with an unsaturated acyl chain recruited PHPLCdelta1 and Gag similarly, whereas PI(4,5)P2 with saturated acyl chains either recruited PHPLCdelta1 but not Gag or sorted these proteins to different phases of vesicles.	pi(4,5)p2	68-77	Pr55(Gag)	Human immunodeficiency virus 1	232-235
25231975-3	2015	TRPM4 Ca(2+) sensitivity is enhanced by calmodulin (CaM) and phosphathydilinositol 4, 5-bisphosphate (PI(4,5)P2) binding, as well as, under certain conditions, PKC activation.	pi(4,5)p2	102-111	transient receptor potential cation channel subfamily M member 4	Homo sapiens	0-5
26123195-11	2015	Overall, our data indicate that TRPM3 is a phosphoinositide-dependent ion channel and that decreasing PI(4,5)P2 abundance limits its activity.	pi(4,5)p2	102-111	transient receptor potential cation channel subfamily M member 3	Homo sapiens	32-37
25349201-6	2015	Furthermore, phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2], an activator of ROMK channels, was detected mainly in the microvilli under resting conditions along with the kinase responsible for PI(4,5)P2 synthesis, phosphatidylinositol-4-phosphate 5-kinase, type I gamma [PI(4)P5K I gamma], which may explain the low basal open probability and increased sensitivity to tetraethylammonium observed here for this channel.	pi(4,5)p2	52-61	potassium inwardly-rectifying channel, subfamily J, member 1	Mus musculus	80-84
25349201-6	2015	Furthermore, phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2], an activator of ROMK channels, was detected mainly in the microvilli under resting conditions along with the kinase responsible for PI(4,5)P2 synthesis, phosphatidylinositol-4-phosphate 5-kinase, type I gamma [PI(4)P5K I gamma], which may explain the low basal open probability and increased sensitivity to tetraethylammonium observed here for this channel.	pi(4,5)p2	196-205	potassium inwardly-rectifying channel, subfamily J, member 1	Mus musculus	80-84
25280637-4	2015	PI(4,5)P2directly regulates vesicle exocytosis by recruiting and activating PI(4,5)P2-binding proteins that regulate SNARE protein function including CAPS, Munc13-1/2, synaptotagmin-1, and other C2 domain-containing proteins.	pi(4,5)p2	0-9	calcyphosine	Homo sapiens	150-154
25280637-4	2015	PI(4,5)P2directly regulates vesicle exocytosis by recruiting and activating PI(4,5)P2-binding proteins that regulate SNARE protein function including CAPS, Munc13-1/2, synaptotagmin-1, and other C2 domain-containing proteins.	pi(4,5)p2	0-9	unc-13 homolog A	Homo sapiens	156-166
25280637-4	2015	PI(4,5)P2directly regulates vesicle exocytosis by recruiting and activating PI(4,5)P2-binding proteins that regulate SNARE protein function including CAPS, Munc13-1/2, synaptotagmin-1, and other C2 domain-containing proteins.	pi(4,5)p2	0-9	synaptotagmin 1	Homo sapiens	168-183
25280637-6	2015	The SNARE protein syntaxin-1 also binds to PI(4,5)P2, which promotes clustering, but an activating role for PI(4,5)P2in syntaxin-1 function remains to be fully characterized.	pi(4,5)p2	43-52	small NF90 (ILF3) associated RNA E	Homo sapiens	4-9
25280637-7	2015	Similar principles underlie polarized constitutive vesicle fusion mediated in part by the PI(4,5)P2-binding subunits of the exocyst complex (Sec3, Exo70).	pi(4,5)p2	90-99	exocyst complex component 1	Homo sapiens	141-145
25280637-7	2015	Similar principles underlie polarized constitutive vesicle fusion mediated in part by the PI(4,5)P2-binding subunits of the exocyst complex (Sec3, Exo70).	pi(4,5)p2	90-99	exocyst complex component 7	Homo sapiens	147-152
25713054-8	2015	PIP5KIbeta mutants whose dimerization was impaired showed a severe decrease in PI(4,5)P2 production and plasma membrane delocalization, although their association to lipid monolayers was unaltered.	pi(4,5)p2	79-88	phosphatidylinositol-4-phosphate 5-kinase type 1 beta	Homo sapiens	0-10
25461777-5	2015	These studies provide insights in the cellular regulation of PTEN, its interaction with PI(4,5)P2 in the inner plasma membrane and the pathway by which its substrate, PI(3,4,5)P3, accesses the PTEN catalytic site.	pi(4,5)p2	88-97	phosphatase and tensin homolog	Homo sapiens	61-65
26150791-11	2015	Thus, Kir6.2, a channel activated by PI(4,5)P2 and PI(4)P was insensitive to VSP.	pi(4,5)p2	37-46	potassium inwardly rectifying channel subfamily J member 11	Homo sapiens	6-12
26150791-12	2015	Surprisingly, despite comparable PI(4,5)P2 affinity as determined by Ci-VSP, the Kv7 and Kir channel families strongly differed in their sensitivity to receptor-mediated depletion of PI(4,5)P2.	pi(4,5)p2	183-192	killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4	Homo sapiens	81-92
25263454-4	2015	Here, we show that these enzymatically competent PTEN mutants fail to translocate to the plasma membrane where PTEN converts PIP3 to PI(4,5)P2.	pi(4,5)p2	133-142	phosphatase and tensin homolog	Homo sapiens	49-53
25263454-4	2015	Here, we show that these enzymatically competent PTEN mutants fail to translocate to the plasma membrane where PTEN converts PIP3 to PI(4,5)P2.	pi(4,5)p2	133-142	phosphatase and tensin homolog	Homo sapiens	111-115
25940347-1	2015	During the late stages of the HIV-1 replication cycle, the viral polyprotein Pr55(Gag) is recruited to the plasma membrane (PM), where it binds phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and directs HIV-1 assembly.	pi(4,5)p2	183-192	Pr55(Gag)	Human immunodeficiency virus 1	82-85
25940347-4	2015	Rab27a also controls PI(4,5)P2 levels at the virus-containing compartments of macrophages.	pi(4,5)p2	21-30	RAB27A, member RAS oncogene family	Homo sapiens	0-6
25940347-6	2015	We conclude that by directing the trafficking of PI4KIIalpha-positive endosomes toward the PM, Rab27a controls PI(4,5)P2 production and, consequently, HIV-1 replication.	pi(4,5)p2	111-120	RAB27A, member RAS oncogene family	Homo sapiens	95-101
25898165-4	2015	Here we show that the membrane-binding domains of two conserved clathrin adaptors, Sla2 and Ent1, co-assemble in a PI(4,5)P2-dependent manner to form organized lattices on membranes.	pi(4,5)p2	115-124	Src like adaptor 2	Homo sapiens	83-87
25918361-2	2015	Here, we used the ability of the TRPV1 ion channel to discriminate between PI(4,5)P2 and PI(4)P to localize the region of TRPV1 sequence that interacts directly with the phosphoinositide.	pi(4,5)p2	75-84	transient receptor potential cation channel subfamily V member 1	Homo sapiens	33-38
25918361-2	2015	Here, we used the ability of the TRPV1 ion channel to discriminate between PI(4,5)P2 and PI(4)P to localize the region of TRPV1 sequence that interacts directly with the phosphoinositide.	pi(4,5)p2	75-84	transient receptor potential cation channel subfamily V member 1	Homo sapiens	122-127
25918361-7	2015	Rather, conversion of a PI(4,5)P2-selective channel to a PI(4)P-selective channel indicates that a structured phosphoinositide-binding site mediates the regulation of TRPV1 activity and that the amino acid at position 721 likely interacts directly with the moiety at the 5" position of the phosphoinositide.	pi(4,5)p2	24-33	transient receptor potential cation channel subfamily V member 1	Homo sapiens	167-172
25852680-3	2015	Despite the extensive biochemical and in vitro studies of Gag and MA binding to membranes over the last two decades, the discovery of the role of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in Gag binding to the PM has sparked a string of studies aimed at elucidating the molecular mechanism of retroviral Gag-PM binding.	pi(4,5)p2	185-194	Pr55(Gag)	Human immunodeficiency virus 1	199-202
25852680-3	2015	Despite the extensive biochemical and in vitro studies of Gag and MA binding to membranes over the last two decades, the discovery of the role of phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in Gag binding to the PM has sparked a string of studies aimed at elucidating the molecular mechanism of retroviral Gag-PM binding.	pi(4,5)p2	185-194	Pr55(Gag)	Human immunodeficiency virus 1	199-202
25781982-8	2015	However, TRPV1 currents were inhibited by simultaneous breakdown of PI(4)P and PI(4,5)P2.	pi(4,5)p2	79-88	transient receptor potential cation channel subfamily V member 1	Homo sapiens	9-14
25781982-12	2015	In conclusion, ASICs and TRPV1 have different sensitivities toward membrane phospholipids, such as PI(4)P, PI(4,5)P2, and AA, although they have common roles as proton sensors.	pi(4,5)p2	107-116	transient receptor potential cation channel subfamily V member 1	Homo sapiens	25-30
25941825-1	2015	Membrane targeting by the Gag proteins of the human immunodeficiency viruses (HIV types-1 and -2) is mediated by Gag"s N-terminally myristylated matrix (MA) domain and is dependent on cellular phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	232-241	gag protein	Feline immunodeficiency virus	26-29
25941825-1	2015	Membrane targeting by the Gag proteins of the human immunodeficiency viruses (HIV types-1 and -2) is mediated by Gag"s N-terminally myristylated matrix (MA) domain and is dependent on cellular phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	232-241	gag protein	Feline immunodeficiency virus	113-116
25941825-7	2015	Depletion of PI(4,5)P2 from the plasma membrane of FIV-infected CRFK cells inhibited production of FIV particles, indicating that, like HIV, FIV hijacks the PI(4,5)P2 cellular signaling system to direct intracellular Gag trafficking during virus assembly.	pi(4,5)p2	13-22	gag protein	Feline immunodeficiency virus	217-220
25869668-6	2015	Sac2/INPP5F and OCRL may cooperate in the sequential dephosphorylation of PI(4,5)P2 at the 5 and 4 position of inositol in a partnership that mimics that of the two phosphatase modules of synaptojanin.	pi(4,5)p2	74-83	Vps52p	Saccharomyces cerevisiae S288C	0-4
25860611-4	2015	We show dynamic membrane contacts between peroxisome and lysosome, which are mediated by lysosomal Synaptotagmin VII binding to the lipid PI(4,5)P2 on peroxisomal membrane.	pi(4,5)p2	138-147	synaptotagmin 7	Homo sapiens	99-116
25898165-4	2015	Here we show that the membrane-binding domains of two conserved clathrin adaptors, Sla2 and Ent1, co-assemble in a PI(4,5)P2-dependent manner to form organized lattices on membranes.	pi(4,5)p2	115-124	solute carrier family 29 member 1 (Augustine blood group)	Homo sapiens	92-96
25568335-4	2015	At phagocytic cups, Rab31 is first recruited during the phosphoinositide transition from PI(4,5)P2 to PI(3,4,5)P3, and it persists on PI(3)P-enriched phagosomes.	pi(4,5)p2	89-98	RAB31, member RAS oncogene family	Homo sapiens	20-25
25305077-3	2015	Loss of OCRL-1, a phosphoinositide-5-phosphatase, leads in Lowe patients" fibroblasts to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) accumulation, with defects in F-actin network, alpha-actinin distribution and ciliogenesis, whereas fibroblasts of Dent-2 patients are still uncharacterized.	pi(4,5)p2	128-137	OCRL inositol polyphosphate-5-phosphatase	Homo sapiens	8-14
25608530-4	2015	Here, we identify a member of the TMEM150 family as a functional homologue of Sfk1 in mammalian cells and demonstrate a role for this protein in the homeostatic regulation of PI(4,5)P2 at the plasma membrane.	pi(4,5)p2	175-184	transmembrane protein 150A	Homo sapiens	34-41
25608530-4	2015	Here, we identify a member of the TMEM150 family as a functional homologue of Sfk1 in mammalian cells and demonstrate a role for this protein in the homeostatic regulation of PI(4,5)P2 at the plasma membrane.	pi(4,5)p2	175-184	Sfk1p	Saccharomyces cerevisiae S288C	78-82
25305077-3	2015	Loss of OCRL-1, a phosphoinositide-5-phosphatase, leads in Lowe patients" fibroblasts to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) accumulation, with defects in F-actin network, alpha-actinin distribution and ciliogenesis, whereas fibroblasts of Dent-2 patients are still uncharacterized.	pi(4,5)p2	128-137	actinin alpha 1	Homo sapiens	186-199
25670203-2	2015	We found that activation of TRPV1 channels with capsaicin either in dorsal root ganglion neurons or in a heterologous expression system inhibited the mechanosensitive Piezo1 and Piezo2 channels by depleting phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and its precursor phosphatidylinositol 4-phosphate [PI(4)P] from the plasma membrane through Ca(2+)-induced phospholipase Cdelta (PLCdelta) activation.	pi(4,5)p2	246-255	transient receptor potential cation channel subfamily V member 1	Homo sapiens	28-33
25518934-3	2015	Hyperosmotic shock promotes calcineurin binding to and dephosphorylation of the PI(4,5)P2 phosphatase synaptojanin/Inp53/Sjl3 and causes dramatic calcineurin-dependent reorganization of PI(4,5)P2-enriched membrane domains.	pi(4,5)p2	80-89	phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53	Saccharomyces cerevisiae S288C	115-120
25518934-3	2015	Hyperosmotic shock promotes calcineurin binding to and dephosphorylation of the PI(4,5)P2 phosphatase synaptojanin/Inp53/Sjl3 and causes dramatic calcineurin-dependent reorganization of PI(4,5)P2-enriched membrane domains.	pi(4,5)p2	186-195	phosphatidylinositol-3-/phosphoinositide 5-phosphatase INP53	Saccharomyces cerevisiae S288C	115-120
25659909-3	2015	A highly basic region of MA targets Gag to the plasma membrane via specific binding to phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	126-135	Pr55(Gag)	Human immunodeficiency virus 1	36-39
25659909-12	2015	Our findings elucidate the mechanism whereby an MA mutant defective in PI(4,5)P2 binding can be rescued and highlight the ability of MA to influence Env glycoprotein function.	pi(4,5)p2	71-80	endogenous retrovirus group K member 20	Homo sapiens	149-152
25670203-2	2015	We found that activation of TRPV1 channels with capsaicin either in dorsal root ganglion neurons or in a heterologous expression system inhibited the mechanosensitive Piezo1 and Piezo2 channels by depleting phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and its precursor phosphatidylinositol 4-phosphate [PI(4)P] from the plasma membrane through Ca(2+)-induced phospholipase Cdelta (PLCdelta) activation.	pi(4,5)p2	246-255	piezo type mechanosensitive ion channel component 1	Homo sapiens	167-173
25670203-2	2015	We found that activation of TRPV1 channels with capsaicin either in dorsal root ganglion neurons or in a heterologous expression system inhibited the mechanosensitive Piezo1 and Piezo2 channels by depleting phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] and its precursor phosphatidylinositol 4-phosphate [PI(4)P] from the plasma membrane through Ca(2+)-induced phospholipase Cdelta (PLCdelta) activation.	pi(4,5)p2	246-255	piezo type mechanosensitive ion channel component 2	Homo sapiens	178-184
26542627-0	2015	TRPM3 joins the ranks of PI(4,5)P2 sensitive ion channels.	pi(4,5)p2	25-34	transient receptor potential cation channel subfamily M member 3	Homo sapiens	0-5
25429968-1	2015	The lipid phosphatase activity of the tumor suppressor phosphatase and tensin homolog (PTEN) is enhanced by the presence of its biological product, phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	187-196	phosphatase and tensin homolog	Homo sapiens	87-91
25704028-1	2015	The human ether-a-go-go-related gene (HERG) potassium current (IHERG) has been shown to decrease in amplitude following stimulation with Gq protein-coupled receptors (GqRs), such as alpha1-adrenergic and M1-muscarinic receptors (alpha1R and M1R, respectively), at least partly via the reduction of membrane phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	346-355	potassium voltage-gated channel subfamily H member 2	Homo sapiens	38-42
25704028-2	2015	The present study was designed to investigate the modulation of HERG channels by PI(4,5)P2 and phosphatidylinositol4-phosphate 5-kinase (PI(4)P5-K), a synthetic enzyme of PI(4,5)P2.	pi(4,5)p2	81-90	potassium voltage-gated channel subfamily H member 2	Homo sapiens	64-68
25704028-2	2015	The present study was designed to investigate the modulation of HERG channels by PI(4,5)P2 and phosphatidylinositol4-phosphate 5-kinase (PI(4)P5-K), a synthetic enzyme of PI(4,5)P2.	pi(4,5)p2	171-180	potassium voltage-gated channel subfamily H member 2	Homo sapiens	64-68
25704028-2	2015	The present study was designed to investigate the modulation of HERG channels by PI(4,5)P2 and phosphatidylinositol4-phosphate 5-kinase (PI(4)P5-K), a synthetic enzyme of PI(4,5)P2.	pi(4,5)p2	171-180	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	95-135
25704028-2	2015	The present study was designed to investigate the modulation of HERG channels by PI(4,5)P2 and phosphatidylinositol4-phosphate 5-kinase (PI(4)P5-K), a synthetic enzyme of PI(4,5)P2.	pi(4,5)p2	171-180	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	137-146
25704028-7	2015	These results suggest that the current density of IHERG is closely dependent on the membrane PI(4,5)P2 level, which is regulated by PI(4)P5-K and GqRs and that replenishing PI(4,5)P2 by PI(4)P5-K recovers IHERG.	pi(4,5)p2	93-102	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	132-141
25704028-7	2015	These results suggest that the current density of IHERG is closely dependent on the membrane PI(4,5)P2 level, which is regulated by PI(4)P5-K and GqRs and that replenishing PI(4,5)P2 by PI(4)P5-K recovers IHERG.	pi(4,5)p2	93-102	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	186-195
25704028-7	2015	These results suggest that the current density of IHERG is closely dependent on the membrane PI(4,5)P2 level, which is regulated by PI(4)P5-K and GqRs and that replenishing PI(4,5)P2 by PI(4)P5-K recovers IHERG.	pi(4,5)p2	173-182	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	186-195
25251606-4	2015	Using mice lacking the ASM gene (ASMko), a model system in NPDA research, we report here that high sphingomyelin levels in mutant neurons lead to low synaptic levels of phosphoinositide PI(4,5)P2 and reduced activity of its hydrolyzing phosphatase PLCgamma, which are key players in synaptic plasticity events.	pi(4,5)p2	186-195	sphingomyelin phosphodiesterase 1, acid lysosomal	Mus musculus	23-26
25425643-0	2015	Molecular determinants of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) binding to transient receptor potential V1 (TRPV1) channels.	pi(4,5)p2	65-74	transient receptor potential cation channel subfamily V member 1	Homo sapiens	87-118
25425643-0	2015	Molecular determinants of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) binding to transient receptor potential V1 (TRPV1) channels.	pi(4,5)p2	65-74	transient receptor potential cation channel subfamily V member 1	Homo sapiens	120-125
25425643-8	2015	Molecular dynamics simulations indicate that PI(4,5)P2 binding induces conformational rearrangements of the structure formed by S6 and the TRP domain, which cause an opening of the lower TRPV1 channel gate.	pi(4,5)p2	45-54	transient receptor potential cation channel subfamily V member 1	Homo sapiens	187-192
25997346-6	2015	Supported lipid bilayers on glass coverslips that contain phosphatidylserine and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) make actin bundles termed filopodia-like structures that contain fascin and have vasodilator-stimulated phosphoprotein (VASP) at their growing tips, indicating that these resemble filopodia growing from the plasma membrane.	pi(4,5)p2	120-129	fascin actin-bundling protein 1 S homeolog	Xenopus laevis	196-202
25997346-6	2015	Supported lipid bilayers on glass coverslips that contain phosphatidylserine and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) make actin bundles termed filopodia-like structures that contain fascin and have vasodilator-stimulated phosphoprotein (VASP) at their growing tips, indicating that these resemble filopodia growing from the plasma membrane.	pi(4,5)p2	120-129	vasodilator stimulated phosphoprotein S homeolog	Xenopus laevis	212-249
25997346-6	2015	Supported lipid bilayers on glass coverslips that contain phosphatidylserine and phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) make actin bundles termed filopodia-like structures that contain fascin and have vasodilator-stimulated phosphoprotein (VASP) at their growing tips, indicating that these resemble filopodia growing from the plasma membrane.	pi(4,5)p2	120-129	vasodilator stimulated phosphoprotein S homeolog	Xenopus laevis	251-255
25251606-5	2015	In addition, mutant neurons have reduced levels of membrane-bound MARCKS, a protein required for PI(4,5)P2 membrane clustering and hydrolysis.	pi(4,5)p2	97-106	myristoylated alanine rich protein kinase C substrate	Mus musculus	66-72
25251606-6	2015	Intracerebroventricular infusion of a peptide that mimics the effector domain of MARCKS increases the content of PI(4,5)P2 in the synaptic membrane and ameliorates behavioral abnormalities in ASMko mice.	pi(4,5)p2	113-122	myristoylated alanine rich protein kinase C substrate	Mus musculus	81-87
25256292-0	2014	TRPV4 channel activity is modulated by direct interaction of the ankyrin domain to PI(4,5)P2.	pi(4,5)p2	83-92	transient receptor potential cation channel subfamily V member 4	Homo sapiens	0-5
25378404-3	2014	The Ca(2+) sensitivity is known to be regulated by calmodulin and membrane phosphoinositides, such as phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2).	pi(4,5)p2	141-150	calmodulin 1	Rattus norvegicus	51-61
25378404-8	2014	Mutations of the acidic amino acids near and in the TRP domain, which are conserved in TRPM2, TRPM5, and TRPM8, deteriorated the Ca(2+) sensitivity in the presence of Co(2+) or PI(4,5)P2 but hardly affected the sensitivity to Co(2+) and PI(4,5)P2.	pi(4,5)p2	177-186	transient receptor potential cation channel, subfamily M, member 2	Rattus norvegicus	87-92
25378404-8	2014	Mutations of the acidic amino acids near and in the TRP domain, which are conserved in TRPM2, TRPM5, and TRPM8, deteriorated the Ca(2+) sensitivity in the presence of Co(2+) or PI(4,5)P2 but hardly affected the sensitivity to Co(2+) and PI(4,5)P2.	pi(4,5)p2	177-186	transient receptor potential cation channel, subfamily M, member 5	Rattus norvegicus	94-99
25378404-8	2014	Mutations of the acidic amino acids near and in the TRP domain, which are conserved in TRPM2, TRPM5, and TRPM8, deteriorated the Ca(2+) sensitivity in the presence of Co(2+) or PI(4,5)P2 but hardly affected the sensitivity to Co(2+) and PI(4,5)P2.	pi(4,5)p2	177-186	transient receptor potential cation channel, subfamily M, member 8	Rattus norvegicus	105-110
25378404-8	2014	Mutations of the acidic amino acids near and in the TRP domain, which are conserved in TRPM2, TRPM5, and TRPM8, deteriorated the Ca(2+) sensitivity in the presence of Co(2+) or PI(4,5)P2 but hardly affected the sensitivity to Co(2+) and PI(4,5)P2.	pi(4,5)p2	237-246	transient receptor potential cation channel, subfamily M, member 2	Rattus norvegicus	87-92
25378404-8	2014	Mutations of the acidic amino acids near and in the TRP domain, which are conserved in TRPM2, TRPM5, and TRPM8, deteriorated the Ca(2+) sensitivity in the presence of Co(2+) or PI(4,5)P2 but hardly affected the sensitivity to Co(2+) and PI(4,5)P2.	pi(4,5)p2	237-246	transient receptor potential cation channel, subfamily M, member 5	Rattus norvegicus	94-99
25378404-8	2014	Mutations of the acidic amino acids near and in the TRP domain, which are conserved in TRPM2, TRPM5, and TRPM8, deteriorated the Ca(2+) sensitivity in the presence of Co(2+) or PI(4,5)P2 but hardly affected the sensitivity to Co(2+) and PI(4,5)P2.	pi(4,5)p2	237-246	transient receptor potential cation channel, subfamily M, member 8	Rattus norvegicus	105-110
25517631-0	2014	Translocation between PI(4,5)P2-poor and PI(4,5)P2-rich microdomains during store depletion determines STIM1 conformation and Orai1 gating.	pi(4,5)p2	22-31	stromal interaction molecule 1	Homo sapiens	103-108
25517631-0	2014	Translocation between PI(4,5)P2-poor and PI(4,5)P2-rich microdomains during store depletion determines STIM1 conformation and Orai1 gating.	pi(4,5)p2	22-31	ORAI calcium release-activated calcium modulator 1	Homo sapiens	126-131
25517631-0	2014	Translocation between PI(4,5)P2-poor and PI(4,5)P2-rich microdomains during store depletion determines STIM1 conformation and Orai1 gating.	pi(4,5)p2	41-50	stromal interaction molecule 1	Homo sapiens	103-108
25517631-0	2014	Translocation between PI(4,5)P2-poor and PI(4,5)P2-rich microdomains during store depletion determines STIM1 conformation and Orai1 gating.	pi(4,5)p2	41-50	ORAI calcium release-activated calcium modulator 1	Homo sapiens	126-131
25517631-4	2014	STIM1-Orai1 must be in a PM/ER microdomain tethered by E-Syt1, stabilized by septin4 and enriched in PI(4,5)P2 for STIM1-SARAF interaction.	pi(4,5)p2	101-110	stromal interaction molecule 1	Homo sapiens	0-5
25517631-4	2014	STIM1-Orai1 must be in a PM/ER microdomain tethered by E-Syt1, stabilized by septin4 and enriched in PI(4,5)P2 for STIM1-SARAF interaction.	pi(4,5)p2	101-110	ORAI calcium release-activated calcium modulator 1	Homo sapiens	6-11
25517631-4	2014	STIM1-Orai1 must be in a PM/ER microdomain tethered by E-Syt1, stabilized by septin4 and enriched in PI(4,5)P2 for STIM1-SARAF interaction.	pi(4,5)p2	101-110	stromal interaction molecule 1	Homo sapiens	115-120
25517631-4	2014	STIM1-Orai1 must be in a PM/ER microdomain tethered by E-Syt1, stabilized by septin4 and enriched in PI(4,5)P2 for STIM1-SARAF interaction.	pi(4,5)p2	101-110	store-operated calcium entry associated regulatory factor	Homo sapiens	121-126
25517631-5	2014	Targeting STIM1 to PI(4,5)P2-rich and -poor microdomains reveals that SARAF-dependent SCDI is observed only when STIM1-Orai1 are within the PI(4,5)P2-rich microdomain.	pi(4,5)p2	19-28	stromal interaction molecule 1	Homo sapiens	10-15
25517631-5	2014	Targeting STIM1 to PI(4,5)P2-rich and -poor microdomains reveals that SARAF-dependent SCDI is observed only when STIM1-Orai1 are within the PI(4,5)P2-rich microdomain.	pi(4,5)p2	19-28	store-operated calcium entry associated regulatory factor	Homo sapiens	70-75
25517631-5	2014	Targeting STIM1 to PI(4,5)P2-rich and -poor microdomains reveals that SARAF-dependent SCDI is observed only when STIM1-Orai1 are within the PI(4,5)P2-rich microdomain.	pi(4,5)p2	19-28	ORAI calcium release-activated calcium modulator 1	Homo sapiens	119-124
25517631-5	2014	Targeting STIM1 to PI(4,5)P2-rich and -poor microdomains reveals that SARAF-dependent SCDI is observed only when STIM1-Orai1 are within the PI(4,5)P2-rich microdomain.	pi(4,5)p2	140-149	stromal interaction molecule 1	Homo sapiens	10-15
25517631-5	2014	Targeting STIM1 to PI(4,5)P2-rich and -poor microdomains reveals that SARAF-dependent SCDI is observed only when STIM1-Orai1 are within the PI(4,5)P2-rich microdomain.	pi(4,5)p2	140-149	store-operated calcium entry associated regulatory factor	Homo sapiens	70-75
25517631-5	2014	Targeting STIM1 to PI(4,5)P2-rich and -poor microdomains reveals that SARAF-dependent SCDI is observed only when STIM1-Orai1 are within the PI(4,5)P2-rich microdomain.	pi(4,5)p2	140-149	ORAI calcium release-activated calcium modulator 1	Homo sapiens	119-124
25517631-6	2014	Notably, store depletion results in transient localization of STIM1-Orai1 in the PI(4,5)P2-poor microdomain, which then translocates to the PI(4,5)P2-rich domain.	pi(4,5)p2	81-90	stromal interaction molecule 1	Homo sapiens	62-67
25517631-6	2014	Notably, store depletion results in transient localization of STIM1-Orai1 in the PI(4,5)P2-poor microdomain, which then translocates to the PI(4,5)P2-rich domain.	pi(4,5)p2	81-90	ORAI calcium release-activated calcium modulator 1	Homo sapiens	68-73
25517631-6	2014	Notably, store depletion results in transient localization of STIM1-Orai1 in the PI(4,5)P2-poor microdomain, which then translocates to the PI(4,5)P2-rich domain.	pi(4,5)p2	140-149	stromal interaction molecule 1	Homo sapiens	62-67
25517631-6	2014	Notably, store depletion results in transient localization of STIM1-Orai1 in the PI(4,5)P2-poor microdomain, which then translocates to the PI(4,5)P2-rich domain.	pi(4,5)p2	140-149	ORAI calcium release-activated calcium modulator 1	Homo sapiens	68-73
25491356-1	2014	UNLABELLED: The matrix (MA) domain of HIV-1 mediates proper Gag localization and membrane binding via interaction with a plasma-membrane (PM)-specific acidic phospholipid, phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2].	pi(4,5)p2	213-222	Pr55(Gag)	Human immunodeficiency virus 1	60-63
25491356-11	2014	IMPORTANCE: MA basic residues in the HIV-1 structural protein Gag interact with phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] and RNA.	pi(4,5)p2	121-130	Pr55(Gag)	Human immunodeficiency virus 1	62-65
25491356-15	2014	In this study, by comparing diverse retroviral MA domains, we elucidated a strong correlation among PI(4,5)P2 dependence, susceptibility to RNA-mediated inhibition, and cellular behaviors of Gag.	pi(4,5)p2	100-109	Pr55(Gag)	Human immunodeficiency virus 1	191-194
24998886-3	2014	Among acidic phospholipids, PI(4,5)P2, a PM-specific phosphoinositide, is essential for proper HIV-1 Gag localization to the PM and efficient virus particle production.	pi(4,5)p2	28-37	Pr55(Gag)	Human immunodeficiency virus 1	101-104
25350771-0	2014	BIN1 membrane curvature sensing and generation show autoinhibition regulated by downstream ligands and PI(4,5)P2.	pi(4,5)p2	103-112	bridging integrator 1	Homo sapiens	0-4
25350771-7	2014	Furthermore, we found that the MC-S&G ability of full-length BIN1 is inhibited on membranes lacking PI(4,5)P2.	pi(4,5)p2	104-113	bridging integrator 1	Homo sapiens	65-69
25350771-8	2014	Addition of PI(4,5)P2 in the membrane activates BIN1 to sense and induce membrane curvature.	pi(4,5)p2	12-21	bridging integrator 1	Homo sapiens	48-52
25350771-10	2014	Addition of SH3 domain ligand (such as PRD peptides), as well as addition of the water-soluble PI(4,5)P2 analogue, can both enhance the MC-S&G ability of BIN1 on membranes without PI(4,5)P2, indicating that the key to activate BIN1 is to disrupt the exon10-SH3 interaction.	pi(4,5)p2	95-104	bridging integrator 1	Homo sapiens	158-162
25350771-10	2014	Addition of SH3 domain ligand (such as PRD peptides), as well as addition of the water-soluble PI(4,5)P2 analogue, can both enhance the MC-S&G ability of BIN1 on membranes without PI(4,5)P2, indicating that the key to activate BIN1 is to disrupt the exon10-SH3 interaction.	pi(4,5)p2	184-193	bridging integrator 1	Homo sapiens	158-162
25313406-7	2014	Similar to Sec3p, the actin-independent localization of Exo70p requires a synergistic interaction with the phosphoinositide PI(4,5)P2.	pi(4,5)p2	124-133	exocyst complex component 1	Homo sapiens	11-16
25313406-7	2014	Similar to Sec3p, the actin-independent localization of Exo70p requires a synergistic interaction with the phosphoinositide PI(4,5)P2.	pi(4,5)p2	124-133	exocyst complex component 7	Homo sapiens	56-62
24389605-0	2014	Structural basis of PI(4,5)P2-dependent regulation of GluA1 by phosphatidylinositol-5-phosphate 4-kinase, type II, alpha (PIP5K2A).	pi(4,5)p2	20-29	glutamate ionotropic receptor AMPA type subunit 1	Homo sapiens	54-59
24389605-0	2014	Structural basis of PI(4,5)P2-dependent regulation of GluA1 by phosphatidylinositol-5-phosphate 4-kinase, type II, alpha (PIP5K2A).	pi(4,5)p2	20-29	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	63-120
24389605-0	2014	Structural basis of PI(4,5)P2-dependent regulation of GluA1 by phosphatidylinositol-5-phosphate 4-kinase, type II, alpha (PIP5K2A).	pi(4,5)p2	20-29	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	122-129
24389605-6	2014	We defined the region K813-K823 of GluA1 as critical for the PI(4,5)P2 effect by performing an alanine scan that suggested PI(4,5)P2 binding to this area.	pi(4,5)p2	61-70	glutamate ionotropic receptor AMPA type subunit 1	Homo sapiens	35-40
24389605-6	2014	We defined the region K813-K823 of GluA1 as critical for the PI(4,5)P2 effect by performing an alanine scan that suggested PI(4,5)P2 binding to this area.	pi(4,5)p2	123-132	glutamate ionotropic receptor AMPA type subunit 1	Homo sapiens	35-40
24389605-7	2014	A PIP strip assay revealed PI(4,5)P2 binding to the C-terminal GluA1 peptide.	pi(4,5)p2	27-36	glutamate ionotropic receptor AMPA type subunit 1	Homo sapiens	63-68
25130443-7	2014	Phosphoinositides such as PI(4,5)P2 were also concentrated at this level together with HIV-1 Gag.	pi(4,5)p2	26-35	Pr55(Gag)	Human immunodeficiency virus 1	93-96
25130443-9	2014	Mobility of PHPLCdelta , a specific probe for PI(4,5)P2 , was reduced > threefold at the level of TNT basis or tip compared with the cell body.	pi(4,5)p2	46-55	chromosome 16 open reading frame 82	Homo sapiens	101-104
25130443-10	2014	CONCLUSION: Our study identified the TNT tip as an active zone of actin cytoskeleton reorganisation with the presence of ezrin, Exo70, N-WASP and PI(4,5)P2 .	pi(4,5)p2	146-155	chromosome 16 open reading frame 82	Homo sapiens	37-40
25256292-4	2014	Among the phosphoinositides, phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) most potently binds to the TRPV4 ARD.	pi(4,5)p2	68-77	transient receptor potential cation channel subfamily V member 4	Homo sapiens	106-111
25256292-5	2014	The crystal structure of the TRPV4 ARD in complex with inositol-1,4,5-trisphosphate, the head-group of PI(4,5)P2, and the molecular-dynamics simulations revealed the PI(4,5)P2-binding amino-acid residues.	pi(4,5)p2	103-112	transient receptor potential cation channel subfamily V member 4	Homo sapiens	29-34
25256292-6	2014	The TRPV4 channel activities were increased by titration or hydrolysis of membrane PI(4,5)P2.	pi(4,5)p2	83-92	transient receptor potential cation channel subfamily V member 4	Homo sapiens	4-9
25256292-7	2014	Notably, disease-associated TRPV4 mutations that cause a gain-of-function phenotype abolished PI(4,5)P2 binding and PI(4,5)P2 sensitivity.	pi(4,5)p2	94-103	transient receptor potential cation channel subfamily V member 4	Homo sapiens	28-33
25256292-7	2014	Notably, disease-associated TRPV4 mutations that cause a gain-of-function phenotype abolished PI(4,5)P2 binding and PI(4,5)P2 sensitivity.	pi(4,5)p2	116-125	transient receptor potential cation channel subfamily V member 4	Homo sapiens	28-33
25256292-8	2014	These findings identify TRPV4 ARD as a lipid-binding domain in which interactions with PI(4,5)P2 normalize the channel activity in TRPV4.	pi(4,5)p2	87-96	transient receptor potential cation channel subfamily V member 4	Homo sapiens	24-29
25256292-8	2014	These findings identify TRPV4 ARD as a lipid-binding domain in which interactions with PI(4,5)P2 normalize the channel activity in TRPV4.	pi(4,5)p2	87-96	transient receptor potential cation channel subfamily V member 4	Homo sapiens	131-136
24834965-5	2014	KEY RESULTS: PI(4,5)P2 and other phospholipids were shown to bind directly to TMEM16A isolated from whole pulmonary artery (PA) and TMEM16A-eGFP expressed in HEK293 cells.	pi(4,5)p2	13-22	anoctamin 1	Homo sapiens	78-85
24834965-5	2014	KEY RESULTS: PI(4,5)P2 and other phospholipids were shown to bind directly to TMEM16A isolated from whole pulmonary artery (PA) and TMEM16A-eGFP expressed in HEK293 cells.	pi(4,5)p2	13-22	anoctamin 1	Homo sapiens	132-139
24843134-10	2014	The decrease in PM PI(4,5)P2 when Sac1 is recruited to the Golgi suggests that the Golgi contribution is ongoing and that PI(4,5)P2 production may be coupled to important cell biological processes such as membrane trafficking or lipid transfer activity.	pi(4,5)p2	19-28	SAC1 like phosphatidylinositide phosphatase	Homo sapiens	34-38
24556334-11	2014	To illustrate the effect of phosphoinositide segregation on protein binding, we show that binding of the tumor suppressor protein PTEN to PI(5)P and PI(4,5)P2 is enhanced in the presence of cholesterol.	pi(4,5)p2	149-158	phosphatase and tensin homolog	Homo sapiens	130-134
25049397-3	2014	Here, using a multidisciplinary approach, including biochemical, biophysical, structural, computational, and cell biology approaches, we provide a detailed view of a multistep activation mechanism of FAK initiated by phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	256-265	protein tyrosine kinase 2	Homo sapiens	200-203
25049397-5	2014	We find PI(4,5)P2 induces clustering of FAK on the lipid bilayer by binding a basic region in the regulatory 4.1, ezrin, radixin, moesin homology (FERM) domain.	pi(4,5)p2	8-17	protein tyrosine kinase 2	Homo sapiens	40-43
25049397-6	2014	In these clusters, PI(4,5)P2 induces a partially open FAK conformation where the autophosphorylation site is exposed, facilitating efficient autophosphorylation and subsequent Src recruitment.	pi(4,5)p2	19-28	protein tyrosine kinase 2	Homo sapiens	54-57
25049397-6	2014	In these clusters, PI(4,5)P2 induces a partially open FAK conformation where the autophosphorylation site is exposed, facilitating efficient autophosphorylation and subsequent Src recruitment.	pi(4,5)p2	19-28	SRC proto-oncogene, non-receptor tyrosine kinase	Homo sapiens	176-179
25049397-7	2014	However, PI(4,5)P2 does not release autoinhibitory interactions; rather, Src phosphorylation of the activation loop in FAK results in release of the FERM/kinase tether and full catalytic activation.	pi(4,5)p2	9-18	protein tyrosine kinase 2	Homo sapiens	119-122
25049397-8	2014	We propose that PI(4,5)P2 and its generation in focal adhesions by the enzyme phosphatidylinositol 4-phosphate 5-kinase type Igamma are important in linking integrin signaling to FAK activation.	pi(4,5)p2	16-25	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	78-119
25049397-8	2014	We propose that PI(4,5)P2 and its generation in focal adhesions by the enzyme phosphatidylinositol 4-phosphate 5-kinase type Igamma are important in linking integrin signaling to FAK activation.	pi(4,5)p2	16-25	protein tyrosine kinase 2	Homo sapiens	179-182
24742366-8	2014	The efficacy of this compound in living cells was validated through its property to enhance actin nucleation at the cell cortex, a PI(4,5)P2 dependent process, and to inhibit PI(4,5)P2 dephosphorylation by OCRL (both overexpressed and endogenous enzyme).	pi(4,5)p2	175-184	OCRL inositol polyphosphate-5-phosphatase	Homo sapiens	206-210
24843134-4	2014	Depleting PI(4)P at the PM with a recruitable 4-phosphatase (Sac1) results in a decrease of PI(4,5)P2 measured by electrical or optical indicators.	pi(4,5)p2	92-101	SAC1 like phosphatidylinositide phosphatase	Homo sapiens	61-65
24979808-3	2014	Using a heterologous expression system in which human PTEN-GFP is expressed in Dictyostelium cells, we identified mutations in the membrane-binding regulatory interface that increase the recruitment of PTEN to the plasma membrane due to enhanced association with PI(4,5)P2.	pi(4,5)p2	263-272	phosphatase and tensin homolog	Homo sapiens	54-58
24979808-3	2014	Using a heterologous expression system in which human PTEN-GFP is expressed in Dictyostelium cells, we identified mutations in the membrane-binding regulatory interface that increase the recruitment of PTEN to the plasma membrane due to enhanced association with PI(4,5)P2.	pi(4,5)p2	263-272	phosphatase and tensin homolog	Homo sapiens	202-206
24843134-10	2014	The decrease in PM PI(4,5)P2 when Sac1 is recruited to the Golgi suggests that the Golgi contribution is ongoing and that PI(4,5)P2 production may be coupled to important cell biological processes such as membrane trafficking or lipid transfer activity.	pi(4,5)p2	122-131	SAC1 like phosphatidylinositide phosphatase	Homo sapiens	34-38
24662006-5	2014	The PH domain of AKT (protein kinase B, PKB) (v-AKT murine thymoma viral oncogene homolog) binds to PI(4,5)P2 and PI(3,4,5)P3, followed by phosphorylation of the Thr308 and Ser473 regulatory sites.	pi(4,5)p2	100-109	thymoma viral proto-oncogene 1	Mus musculus	17-20
24662006-5	2014	The PH domain of AKT (protein kinase B, PKB) (v-AKT murine thymoma viral oncogene homolog) binds to PI(4,5)P2 and PI(3,4,5)P3, followed by phosphorylation of the Thr308 and Ser473 regulatory sites.	pi(4,5)p2	100-109	thymoma viral proto-oncogene 1	Mus musculus	40-43
24662006-5	2014	The PH domain of AKT (protein kinase B, PKB) (v-AKT murine thymoma viral oncogene homolog) binds to PI(4,5)P2 and PI(3,4,5)P3, followed by phosphorylation of the Thr308 and Ser473 regulatory sites.	pi(4,5)p2	100-109	AKT serine/threonine kinase 1	Homo sapiens	48-51
24813885-5	2014	Accordingly, we found that inhibiting the formation of PI(5)P and PI(4,5)P2 specifically inhibits tumor necrosis factor (TNF)-mediated necroptosis but not apoptosis.	pi(4,5)p2	66-75	tumor necrosis factor	Homo sapiens	98-119
24813885-5	2014	Accordingly, we found that inhibiting the formation of PI(5)P and PI(4,5)P2 specifically inhibits tumor necrosis factor (TNF)-mediated necroptosis but not apoptosis.	pi(4,5)p2	66-75	tumor necrosis factor	Homo sapiens	121-124
24904548-0	2014	Tsg101 regulates PI(4,5)P2/Ca(2+) signaling for HIV-1 Gag assembly.	pi(4,5)p2	17-26	tumor susceptibility 101	Homo sapiens	0-6
24768049-0	2014	PI(4,5)P2 produced by the PI4P5K SKTL controls apical size by tethering PAR-3 in Drosophila epithelial cells.	pi(4,5)p2	0-9	skittles	Drosophila melanogaster	33-37
24768049-0	2014	PI(4,5)P2 produced by the PI4P5K SKTL controls apical size by tethering PAR-3 in Drosophila epithelial cells.	pi(4,5)p2	0-9	bazooka	Drosophila melanogaster	72-77
24768049-5	2014	PI(4,5)P2 is essentially regulated by the PI4P5 kinase Skittles (SKTL), whereas neither the phosphatase PTEN nor the PI(4,5)P3 kinase DP110 lead to loss of apical-basal polarity.	pi(4,5)p2	0-9	skittles	Drosophila melanogaster	65-69
24768049-6	2014	By inactivating SKTL and thereby strongly reducing PI(4,5)P2 levels in a single cell of the epithelium, we observe the disassembly of adherens junctions, actin cytoskeleton reorganization, and apical constriction leading to delamination, a process similar to that observed during epithelial-mesenchymal transition.	pi(4,5)p2	51-60	skittles	Drosophila melanogaster	16-20
24768049-7	2014	We provide evidence that PI(4,5)P2 controls the apical targeting of PAR-3/Bazooka to the plasma membrane and that the loss of this polarized distribution is sufficient to induce a similar cell shape change.	pi(4,5)p2	25-34	bazooka	Drosophila melanogaster	68-73
24768049-7	2014	We provide evidence that PI(4,5)P2 controls the apical targeting of PAR-3/Bazooka to the plasma membrane and that the loss of this polarized distribution is sufficient to induce a similar cell shape change.	pi(4,5)p2	25-34	bazooka	Drosophila melanogaster	74-81
24610942-3	2014	PI(4,5)P2 serves as a fundamental regulator of E-cadherin transport, and PI(4,5)P2-generating enzymes are important signaling relays in these pathways.	pi(4,5)p2	0-9	cadherin 1	Homo sapiens	47-57
24798734-6	2014	PI(4,5)P2-mediated contractility is counteracted by PI(3,4,5)P3 and the zygotic gene bottleneck, which acts by limiting myosin recruitment during plasma membrane expansion.	pi(4,5)p2	0-9	zipper	Drosophila melanogaster	120-126
24904548-0	2014	Tsg101 regulates PI(4,5)P2/Ca(2+) signaling for HIV-1 Gag assembly.	pi(4,5)p2	17-26	Pr55(Gag)	Human immunodeficiency virus 1	54-57
24904548-11	2014	Gag expression was also accompanied by increased PI(4,5)P2 accumulation at the plasma membrane, a condition favoring store refilling capacity.	pi(4,5)p2	49-58	Pr55(Gag)	Human immunodeficiency virus 1	0-3
24599956-2	2014	Here we show that the signaling lipid phosphoinositide 4,5-bisphosphate (PI(4,5)P2) has opposite effects on the function of TRPV1 ion channels depending on which leaflet of the cell membrane it resides in.	pi(4,5)p2	73-82	transient receptor potential cation channel subfamily V member 1	Homo sapiens	124-129
24599956-3	2014	We observed potentiation of capsaicin-activated TRPV1 currents by PI(4,5)P2 in the intracellular leaflet of the plasma membrane but inhibition of capsaicin-activated currents when PI(4,5)P2 was in both leaflets of the membrane, although much higher concentrations of PI(4,5)P2 in the extracellular leaflet were required for inhibition compared with the concentrations of PI(4,5)P2 in the intracellular leaflet that produced activation.	pi(4,5)p2	66-75	transient receptor potential cation channel subfamily V member 1	Homo sapiens	48-53
24599956-5	2014	The asymmetry-dependent effect of PI(4,5)P2 may resolve the long standing controversy about whether PI(4,5)P2 is an activator or inhibitor of TRPV1.	pi(4,5)p2	34-43	transient receptor potential cation channel subfamily V member 1	Homo sapiens	142-147
24186361-4	2014	Synaptojanin 1 (Synj1), a lipid phosphatase mediating the breakdown of PI(4,5)P2, has been shown to play a role in synaptic vesicle recycling and receptor trafficking in neurons.	pi(4,5)p2	71-80	synaptojanin 1	Mus musculus	0-14
24821989-0	2014	PI(4,5)P2 regulates myoblast fusion through Arp2/3 regulator localization at the fusion site.	pi(4,5)p2	0-9	Actin-related protein 2	Drosophila melanogaster	44-48
24821989-6	2014	Regulators of actin branching via Arp2/3 colocalize with PI(4,5)P2 in vivo and bind PI(4,5)P2 in vitro.	pi(4,5)p2	57-66	Actin-related protein 2	Drosophila melanogaster	34-38
24821989-6	2014	Regulators of actin branching via Arp2/3 colocalize with PI(4,5)P2 in vivo and bind PI(4,5)P2 in vitro.	pi(4,5)p2	84-93	Actin-related protein 2	Drosophila melanogaster	34-38
24821989-7	2014	Manipulation of PI(4,5)P2 availability leads to impaired fusion, with a reduction in the F-actin focus size and altered focus morphology.	pi(4,5)p2	16-25	Actin 79B	Drosophila melanogaster	89-96
24186361-4	2014	Synaptojanin 1 (Synj1), a lipid phosphatase mediating the breakdown of PI(4,5)P2, has been shown to play a role in synaptic vesicle recycling and receptor trafficking in neurons.	pi(4,5)p2	71-80	synaptojanin 1	Mus musculus	16-21
24186361-7	2014	To this end, we developed a screening assay for Synj1 based on detection of inorganic phosphate liberation from a water-soluble, short-chain PI(4,5)P2.	pi(4,5)p2	141-150	synaptojanin 1	Mus musculus	48-53
24022404-2	2014	Protein kinase C (PKC) and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] are obligatory for native TRPC1 channel activation in vascular smooth muscle cells (VSMCs) but how PKC and PI(4,5)P2 act together to induce channel gating remains unresolved.	pi(4,5)p2	66-75	transient receptor potential cation channel subfamily C member 1	Homo sapiens	103-108
24434583-0	2014	A Pil1-Sle1-Syj1-Tax4 functional pathway links eisosomes with PI(4,5)P2 regulation.	pi(4,5)p2	62-71	lipid-binding protein PIL1	Saccharomyces cerevisiae S288C	2-6
24434583-0	2014	A Pil1-Sle1-Syj1-Tax4 functional pathway links eisosomes with PI(4,5)P2 regulation.	pi(4,5)p2	62-71	Tax4p	Saccharomyces cerevisiae S288C	17-21
24434583-5	2014	Defects in PI(4,5)P2 regulation led to eisosome defects, and we found that the core eisosome protein Pil1 can bind to and tubulate liposomes containing PI(4,5)P2.	pi(4,5)p2	11-20	lipid-binding protein PIL1	Saccharomyces cerevisiae S288C	101-105
24434583-5	2014	Defects in PI(4,5)P2 regulation led to eisosome defects, and we found that the core eisosome protein Pil1 can bind to and tubulate liposomes containing PI(4,5)P2.	pi(4,5)p2	152-161	lipid-binding protein PIL1	Saccharomyces cerevisiae S288C	101-105
24434583-6	2014	Mutations in components of the Pil1-Sle1-Syj1-Tax4 pathway suppress the growth and morphology defects of TORC2 mutants, indicating that eisosome-dependent regulation of PI(4,5)P2 feeds into signal transduction pathways.	pi(4,5)p2	169-178	lipid-binding protein PIL1	Saccharomyces cerevisiae S288C	31-35
24434583-6	2014	Mutations in components of the Pil1-Sle1-Syj1-Tax4 pathway suppress the growth and morphology defects of TORC2 mutants, indicating that eisosome-dependent regulation of PI(4,5)P2 feeds into signal transduction pathways.	pi(4,5)p2	169-178	Tax4p	Saccharomyces cerevisiae S288C	46-50
24413173-2	2014	Here, we show that cytosolic phospholipase A2epsilon (cPLA2epsilon, also known as PLA2G4E) is targeted to the membrane compartments of the clathrin-independent endocytic route through a C-terminal stretch of positively charged amino acids, which allows the enzyme to interact with phosphoinositide lipids [especially PI(4,5)P2] that are enriched in clathrin-independent endosomes.	pi(4,5)p2	317-326	phospholipase A2 group IVE	Homo sapiens	19-52
24413173-2	2014	Here, we show that cytosolic phospholipase A2epsilon (cPLA2epsilon, also known as PLA2G4E) is targeted to the membrane compartments of the clathrin-independent endocytic route through a C-terminal stretch of positively charged amino acids, which allows the enzyme to interact with phosphoinositide lipids [especially PI(4,5)P2] that are enriched in clathrin-independent endosomes.	pi(4,5)p2	317-326	phospholipase A2 group IVE	Homo sapiens	54-66
24413173-2	2014	Here, we show that cytosolic phospholipase A2epsilon (cPLA2epsilon, also known as PLA2G4E) is targeted to the membrane compartments of the clathrin-independent endocytic route through a C-terminal stretch of positively charged amino acids, which allows the enzyme to interact with phosphoinositide lipids [especially PI(4,5)P2] that are enriched in clathrin-independent endosomes.	pi(4,5)p2	317-326	phospholipase A2 group IVE	Homo sapiens	82-89
24531551-0	2014	Lipotoxic disruption of NHE1 interaction with PI(4,5)P2 expedites proximal tubule apoptosis.	pi(4,5)p2	46-55	solute carrier family 9 (sodium/hydrogen exchanger), member 1	Mus musculus	24-28
24531551-2	2014	The Na+/H+ exchanger NHE1 regulates proximal tubule cell survival through interaction with phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], but pathophysiologic triggers for NHE1 inactivation are unknown.	pi(4,5)p2	130-139	solute carrier family 9 (sodium/hydrogen exchanger), member 1	Mus musculus	21-25
24531551-5	2014	LC-CoAs and the cytosolic domain of NHE1 directly interacted, with an affinity comparable to that of the PI(4,5)P2-NHE1 interaction, and competing LC-CoAs disrupted binding of the NHE1 cytosolic tail to PI(4,5)P2.	pi(4,5)p2	105-114	solute carrier family 9 (sodium/hydrogen exchanger), member 1	Mus musculus	36-40
24531551-5	2014	LC-CoAs and the cytosolic domain of NHE1 directly interacted, with an affinity comparable to that of the PI(4,5)P2-NHE1 interaction, and competing LC-CoAs disrupted binding of the NHE1 cytosolic tail to PI(4,5)P2.	pi(4,5)p2	105-114	solute carrier family 9 (sodium/hydrogen exchanger), member 1	Mus musculus	115-119
24531551-5	2014	LC-CoAs and the cytosolic domain of NHE1 directly interacted, with an affinity comparable to that of the PI(4,5)P2-NHE1 interaction, and competing LC-CoAs disrupted binding of the NHE1 cytosolic tail to PI(4,5)P2.	pi(4,5)p2	105-114	solute carrier family 9 (sodium/hydrogen exchanger), member 1	Mus musculus	115-119
24531551-5	2014	LC-CoAs and the cytosolic domain of NHE1 directly interacted, with an affinity comparable to that of the PI(4,5)P2-NHE1 interaction, and competing LC-CoAs disrupted binding of the NHE1 cytosolic tail to PI(4,5)P2.	pi(4,5)p2	203-212	solute carrier family 9 (sodium/hydrogen exchanger), member 1	Mus musculus	36-40
24409153-3	2014	Different Kir isoforms display distinct specificities for the activating PIPs but all eukaryotic Kir channels are activated by PI(4,5)P2.	pi(4,5)p2	127-136	killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4	Homo sapiens	10-13
24409153-3	2014	Different Kir isoforms display distinct specificities for the activating PIPs but all eukaryotic Kir channels are activated by PI(4,5)P2.	pi(4,5)p2	127-136	killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4	Homo sapiens	97-100
24409153-5	2014	Recent crystal structures of eukaryotic Kir channels in apo and lipid bound forms reveal one specific binding site per subunit, formed at the interface of N- and C-terminal domains, just beyond the transmembrane segments and clearly involving some of the key residues previously identified as controlling PI(4,5)P2 sensitivity.	pi(4,5)p2	305-314	killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4	Homo sapiens	40-43
24470487-0	2014	PLC-mediated PI(4,5)P2 hydrolysis regulates activation and inactivation of TRPC6/7 channels.	pi(4,5)p2	13-22	transient receptor potential cation channel subfamily C member 6	Homo sapiens	75-80
24470487-1	2014	Transient receptor potential classical (or canonical) (TRPC)3, TRPC6, and TRPC7 are a subfamily of TRPC channels activated by diacylglycerol (DAG) produced through the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) by phospholipase C (PLC).	pi(4,5)p2	221-230	transient receptor potential cation channel subfamily C member 3	Homo sapiens	55-61
24470487-1	2014	Transient receptor potential classical (or canonical) (TRPC)3, TRPC6, and TRPC7 are a subfamily of TRPC channels activated by diacylglycerol (DAG) produced through the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) by phospholipase C (PLC).	pi(4,5)p2	221-230	transient receptor potential cation channel subfamily C member 6	Homo sapiens	63-68
24470487-1	2014	Transient receptor potential classical (or canonical) (TRPC)3, TRPC6, and TRPC7 are a subfamily of TRPC channels activated by diacylglycerol (DAG) produced through the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) by phospholipase C (PLC).	pi(4,5)p2	221-230	transient receptor potential cation channel subfamily C member 7	Homo sapiens	74-79
24470487-2	2014	PI(4,5)P2 depletion by a heterologously expressed phosphatase inhibits TRPC3, TRPC6, and TRPC7 activity independently of DAG; however, the physiological role of PI(4,5)P2 reduction on channel activity remains unclear.	pi(4,5)p2	0-9	transient receptor potential cation channel subfamily C member 3	Homo sapiens	71-76
24470487-2	2014	PI(4,5)P2 depletion by a heterologously expressed phosphatase inhibits TRPC3, TRPC6, and TRPC7 activity independently of DAG; however, the physiological role of PI(4,5)P2 reduction on channel activity remains unclear.	pi(4,5)p2	0-9	transient receptor potential cation channel subfamily C member 6	Homo sapiens	78-83
24470487-2	2014	PI(4,5)P2 depletion by a heterologously expressed phosphatase inhibits TRPC3, TRPC6, and TRPC7 activity independently of DAG; however, the physiological role of PI(4,5)P2 reduction on channel activity remains unclear.	pi(4,5)p2	0-9	transient receptor potential cation channel subfamily C member 7	Homo sapiens	89-94
24470487-4	2014	Measurements made at different levels of receptor activation revealed a correlation between the kinetics of PI(4,5)P2 reduction and those of receptor-operated TRPC6 and TRPC7 current activation and inactivation.	pi(4,5)p2	108-117	transient receptor potential cation channel subfamily C member 6	Homo sapiens	159-164
24470487-4	2014	Measurements made at different levels of receptor activation revealed a correlation between the kinetics of PI(4,5)P2 reduction and those of receptor-operated TRPC6 and TRPC7 current activation and inactivation.	pi(4,5)p2	108-117	transient receptor potential cation channel subfamily C member 7	Homo sapiens	169-174
24470487-10	2014	In conclusion, our studies demonstrate a fundamental role for PI(4,5)P2 in regulating TRPC6 and TRPC7 activity triggered by PLC-coupled receptor stimulation.	pi(4,5)p2	62-71	transient receptor potential cation channel subfamily C member 6	Homo sapiens	86-91
24470487-10	2014	In conclusion, our studies demonstrate a fundamental role for PI(4,5)P2 in regulating TRPC6 and TRPC7 activity triggered by PLC-coupled receptor stimulation.	pi(4,5)p2	62-71	transient receptor potential cation channel subfamily C member 7	Homo sapiens	96-101
24756708-5	2014	The N- and C- termini have residues and regions that are sites for phosphorylation/dephosphorylation and PI(4,5)P2 binding, which regulate TRPV1 sensitivity and membrane insertion.	pi(4,5)p2	105-114	transient receptor potential cation channel subfamily V member 1	Homo sapiens	139-144
24560147-4	2014	Gq protein-coupled receptors of the plasma membrane activate phospholipase C (PLC) which cleaves the minor plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) into the second messengers diacylgycerol and inositol 1,4,5-trisphosphate, leading to calcium release, protein kinase C (PKC) activation, and PI(4,5)P2 depletion.	pi(4,5)p2	168-177	proline rich transmembrane protein 2	Homo sapiens	282-298
24560147-4	2014	Gq protein-coupled receptors of the plasma membrane activate phospholipase C (PLC) which cleaves the minor plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) into the second messengers diacylgycerol and inositol 1,4,5-trisphosphate, leading to calcium release, protein kinase C (PKC) activation, and PI(4,5)P2 depletion.	pi(4,5)p2	168-177	proline rich transmembrane protein 2	Homo sapiens	300-303
24560147-4	2014	Gq protein-coupled receptors of the plasma membrane activate phospholipase C (PLC) which cleaves the minor plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) into the second messengers diacylgycerol and inositol 1,4,5-trisphosphate, leading to calcium release, protein kinase C (PKC) activation, and PI(4,5)P2 depletion.	pi(4,5)p2	321-330	proline rich transmembrane protein 2	Homo sapiens	282-298
24560147-4	2014	Gq protein-coupled receptors of the plasma membrane activate phospholipase C (PLC) which cleaves the minor plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) into the second messengers diacylgycerol and inositol 1,4,5-trisphosphate, leading to calcium release, protein kinase C (PKC) activation, and PI(4,5)P2 depletion.	pi(4,5)p2	321-330	proline rich transmembrane protein 2	Homo sapiens	300-303
24022404-2	2014	Protein kinase C (PKC) and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] are obligatory for native TRPC1 channel activation in vascular smooth muscle cells (VSMCs) but how PKC and PI(4,5)P2 act together to induce channel gating remains unresolved.	pi(4,5)p2	184-193	transient receptor potential cation channel subfamily C member 1	Homo sapiens	103-108
24022404-3	2014	The present study reveals that myristoylated alanine-rich C kinase substrate (MARCKS) protein coordinates activation of TRPC1 channels by PKC and PI(4,5)P2.	pi(4,5)p2	146-155	myristoylated alanine rich protein kinase C substrate	Homo sapiens	31-76
24022404-3	2014	The present study reveals that myristoylated alanine-rich C kinase substrate (MARCKS) protein coordinates activation of TRPC1 channels by PKC and PI(4,5)P2.	pi(4,5)p2	146-155	myristoylated alanine rich protein kinase C substrate	Homo sapiens	78-84
24022404-3	2014	The present study reveals that myristoylated alanine-rich C kinase substrate (MARCKS) protein coordinates activation of TRPC1 channels by PKC and PI(4,5)P2.	pi(4,5)p2	146-155	transient receptor potential cation channel subfamily C member 1	Homo sapiens	120-125
24022404-4	2014	TRPC1 channels and MARCKS form signaling complexes with PI(4,5)P2 bound to MARCKS; in this configuration TRPC1 channels are closed.	pi(4,5)p2	56-65	transient receptor potential cation channel subfamily C member 1	Homo sapiens	0-5
24022404-4	2014	TRPC1 channels and MARCKS form signaling complexes with PI(4,5)P2 bound to MARCKS; in this configuration TRPC1 channels are closed.	pi(4,5)p2	56-65	myristoylated alanine rich protein kinase C substrate	Homo sapiens	19-25
24022404-4	2014	TRPC1 channels and MARCKS form signaling complexes with PI(4,5)P2 bound to MARCKS; in this configuration TRPC1 channels are closed.	pi(4,5)p2	56-65	myristoylated alanine rich protein kinase C substrate	Homo sapiens	75-81
24022404-4	2014	TRPC1 channels and MARCKS form signaling complexes with PI(4,5)P2 bound to MARCKS; in this configuration TRPC1 channels are closed.	pi(4,5)p2	56-65	transient receptor potential cation channel subfamily C member 1	Homo sapiens	105-110
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	146-155	transient receptor potential cation channel subfamily C member 1	Homo sapiens	14-19
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	146-155	transient receptor potential cation channel subfamily C member 1	Homo sapiens	59-64
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	146-155	transient receptor potential cation channel subfamily C member 1	Homo sapiens	59-64
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	146-155	myristoylated alanine rich protein kinase C substrate	Homo sapiens	161-167
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	146-155	transient receptor potential cation channel subfamily C member 1	Homo sapiens	59-64
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	169-178	transient receptor potential cation channel subfamily C member 1	Homo sapiens	14-19
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	169-178	transient receptor potential cation channel subfamily C member 1	Homo sapiens	59-64
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	169-178	transient receptor potential cation channel subfamily C member 1	Homo sapiens	59-64
24022404-5	2014	Activators of TRPC1 channels induce PKC phosphorylation of TRPC1 proteins, which causes dissociation of TRPC1 subunits from MARCKS and release of PI(4,5)P2 from MARCKS; PI(4,5)P2 subsequently binds to TRPC1 subunits to induce channel opening.	pi(4,5)p2	169-178	transient receptor potential cation channel subfamily C member 1	Homo sapiens	59-64
24022404-6	2014	Calmodulin acting at, or upstream of, MARCKS is also required for TRPC1 channel opening through a similar gating mechanism involving PKC and PI(4,5)P2.	pi(4,5)p2	141-150	calmodulin 1	Homo sapiens	0-10
24022404-6	2014	Calmodulin acting at, or upstream of, MARCKS is also required for TRPC1 channel opening through a similar gating mechanism involving PKC and PI(4,5)P2.	pi(4,5)p2	141-150	myristoylated alanine rich protein kinase C substrate	Homo sapiens	38-44
24022404-6	2014	Calmodulin acting at, or upstream of, MARCKS is also required for TRPC1 channel opening through a similar gating mechanism involving PKC and PI(4,5)P2.	pi(4,5)p2	141-150	transient receptor potential cation channel subfamily C member 1	Homo sapiens	66-71
24022404-8	2014	Moreover, our data provide evidence that PI(4,5)P2 is a gating ligand of TRPC1 channels.	pi(4,5)p2	41-50	transient receptor potential cation channel subfamily C member 1	Homo sapiens	73-78
26437838-5	2014	Recent studies reveled that PM-specific phospholipid PI(4,5)P2 plays an important role in directing Gag to the PM through its interaction with MA.	pi(4,5)p2	53-62	Pr55(Gag)	Human immunodeficiency virus 1	100-103
24302762-0	2013	Structural insights into the Ca2+ and PI(4,5)P2 binding modes of the C2 domains of rabphilin 3A and synaptotagmin 1.	pi(4,5)p2	38-47	rabphilin 3A	Homo sapiens	83-95
24302762-0	2013	Structural insights into the Ca2+ and PI(4,5)P2 binding modes of the C2 domains of rabphilin 3A and synaptotagmin 1.	pi(4,5)p2	38-47	synaptotagmin 1	Homo sapiens	100-115
24302762-5	2013	Structural comparisons between C2A domains of rabphilin 3A and synaptotagmin 1 indicated the presence of a key glutamic residue in the polybasic cluster of synaptotagmin 1 that abolishes the interaction with PI(4,5)P2.	pi(4,5)p2	208-217	rabphilin 3A	Homo sapiens	46-58
24302762-5	2013	Structural comparisons between C2A domains of rabphilin 3A and synaptotagmin 1 indicated the presence of a key glutamic residue in the polybasic cluster of synaptotagmin 1 that abolishes the interaction with PI(4,5)P2.	pi(4,5)p2	208-217	synaptotagmin 1	Homo sapiens	63-78
24302762-5	2013	Structural comparisons between C2A domains of rabphilin 3A and synaptotagmin 1 indicated the presence of a key glutamic residue in the polybasic cluster of synaptotagmin 1 that abolishes the interaction with PI(4,5)P2.	pi(4,5)p2	208-217	synaptotagmin 1	Homo sapiens	156-171
23926047-4	2013	Syndapin colocalizes with phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) at the cleavage furrow, where it directly interacts with a contractile ring component, Anillin.	pi(4,5)p2	65-74	Syndapin	Drosophila melanogaster	0-8
24052255-1	2013	Recent studies link synaptojanin 1 (synj1), the main phosphoinositol (4,5)-biphosphate phosphatase (PI(4,5)P2-degrading enzyme) in the brain and synapses, to Alzheimer disease.	pi(4,5)p2	100-109	synaptojanin 1	Mus musculus	20-34
24052255-1	2013	Recent studies link synaptojanin 1 (synj1), the main phosphoinositol (4,5)-biphosphate phosphatase (PI(4,5)P2-degrading enzyme) in the brain and synapses, to Alzheimer disease.	pi(4,5)p2	100-109	synaptojanin 1	Mus musculus	36-41
24052255-8	2013	These effects are partially dependent upon elevated PI(4,5)P2 with synj1 down-regulation.	pi(4,5)p2	52-61	synaptojanin 1	Mus musculus	67-72
24052255-9	2013	In summary, our data suggest a novel mechanism by which reduction of a PI(4,5)P2-degrading enzyme, synj1, improves amyloid-induced neuropathology and behavior deficits through accelerating cellular Abeta clearance.	pi(4,5)p2	71-80	synaptojanin 1	Mus musculus	99-104
24052255-9	2013	In summary, our data suggest a novel mechanism by which reduction of a PI(4,5)P2-degrading enzyme, synj1, improves amyloid-induced neuropathology and behavior deficits through accelerating cellular Abeta clearance.	pi(4,5)p2	71-80	amyloid beta (A4) precursor protein	Mus musculus	198-203
24044355-4	2013	We found that tetramerization of the STIM1 K-rich domain is necessary for efficient binding to PI(4,5)P2-containing PM-like liposomes consistent with an oligomerization-driven STIM1 activation.	pi(4,5)p2	95-104	stromal interaction molecule 1	Homo sapiens	37-42
24044355-4	2013	We found that tetramerization of the STIM1 K-rich domain is necessary for efficient binding to PI(4,5)P2-containing PM-like liposomes consistent with an oligomerization-driven STIM1 activation.	pi(4,5)p2	95-104	stromal interaction molecule 1	Homo sapiens	176-181
24044355-7	2013	These distinct features of the STIM2 K-rich domain cause an increased affinity for PI(4,5)P2, consistent with the lower activation threshold of STIM2 and a function as regulator of basal Ca2+ levels.	pi(4,5)p2	83-92	stromal interaction molecule 2	Homo sapiens	31-36
24044355-7	2013	These distinct features of the STIM2 K-rich domain cause an increased affinity for PI(4,5)P2, consistent with the lower activation threshold of STIM2 and a function as regulator of basal Ca2+ levels.	pi(4,5)p2	83-92	stromal interaction molecule 2	Homo sapiens	144-149
24044355-8	2013	Concomitant with higher affinity for PM lipids, binding of CaM (calmodulin) inhibited the interaction of the STIM2 K-rich domain with liposomes in a Ca2+ and PI(4,5)P2 concentration-dependent manner.	pi(4,5)p2	158-167	calmodulin 1	Homo sapiens	59-62
24044355-8	2013	Concomitant with higher affinity for PM lipids, binding of CaM (calmodulin) inhibited the interaction of the STIM2 K-rich domain with liposomes in a Ca2+ and PI(4,5)P2 concentration-dependent manner.	pi(4,5)p2	158-167	calmodulin 1	Homo sapiens	64-74
24044355-8	2013	Concomitant with higher affinity for PM lipids, binding of CaM (calmodulin) inhibited the interaction of the STIM2 K-rich domain with liposomes in a Ca2+ and PI(4,5)P2 concentration-dependent manner.	pi(4,5)p2	158-167	stromal interaction molecule 2	Homo sapiens	109-114
24265857-6	2013	Conversely, ectopic overexpression of PIP4K2alpha would consume PI5P to produce PI(4,5)P2 and we found that overexpressing PIP4K2alpha decreased cell migration speed.	pi(4,5)p2	80-89	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	38-49
24265857-6	2013	Conversely, ectopic overexpression of PIP4K2alpha would consume PI5P to produce PI(4,5)P2 and we found that overexpressing PIP4K2alpha decreased cell migration speed.	pi(4,5)p2	80-89	phosphatidylinositol-5-phosphate 4-kinase type 2 alpha	Homo sapiens	123-134
24314079-6	2013	In particular, we find that TRPV1 is more selective for PI(4,5)P2 than PI(4)P and activated by extremely low membrane mole fractions of PIPs.	pi(4,5)p2	56-65	transient receptor potential cation channel subfamily V member 1	Homo sapiens	28-33
24123515-7	2013	Phospholipase C-mediated hydrolysis of the phosphoinositide PI(4,5)P2 , a necessary cofactor for P2X4 channel function, underlies this inhibitory crosstalk.	pi(4,5)p2	60-69	purinergic receptor P2X 4	Homo sapiens	97-101
23897088-5	2013	We further show that depletion of Nir2 substantially reduces the PI(4,5)P2 levels at the plasma membrane and concomitantly GF-stimulated PI(3,4,5)P3 production.	pi(4,5)p2	65-74	phosphatidylinositol transfer protein membrane associated 1	Homo sapiens	34-38
23861394-3	2013	Here we analyzed the ability of one peripherally associated membrane protein, annexin A2 (AnxA2), to induce the formation of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2)-rich domains in giant unilamellar vesicles (GUVs) of complex lipid composition.	pi(4,5)p2	164-173	annexin A2	Homo sapiens	78-88
23861394-3	2013	Here we analyzed the ability of one peripherally associated membrane protein, annexin A2 (AnxA2), to induce the formation of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2)-rich domains in giant unilamellar vesicles (GUVs) of complex lipid composition.	pi(4,5)p2	164-173	annexin A2	Homo sapiens	90-95
23861394-4	2013	AnxA2 is a cytosolic protein that can bind PI(4,5)P2 and other acidic phospholipids in a Ca(2+)-dependent manner and that has been implicated in cellular membrane dynamics in endocytosis and exocytosis.	pi(4,5)p2	43-52	annexin A2	Homo sapiens	0-5
23861394-5	2013	We show that AnxA2 binding to GUVs induces lipid phase separation and the recruitment of PI(4,5)P2, cholesterol and glycosphingolipids into larger clusters.	pi(4,5)p2	89-98	annexin A2	Homo sapiens	13-18
23861394-7	2013	All AnxA2 derivatives inducing PI(4,5)P2 clustering are also capable of forming interconnections between PI(4,5)P2-rich microdomains of adjacent GUVs.	pi(4,5)p2	31-40	annexin A2	Homo sapiens	4-9
23861394-7	2013	All AnxA2 derivatives inducing PI(4,5)P2 clustering are also capable of forming interconnections between PI(4,5)P2-rich microdomains of adjacent GUVs.	pi(4,5)p2	105-114	annexin A2	Homo sapiens	4-9
23417604-4	2013	A shortage of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] in Galphaq (Q209L) may be responsible for reduced TRPC4 activity.	pi(4,5)p2	53-62	G protein subunit alpha q	Homo sapiens	67-74
23552101-7	2013	PIP5K1B encodes phosphatidylinositol 4-phosphate 5-kinase beta type I (pip5k1beta), an enzyme functionally linked to actin cytoskeleton dynamics that phosphorylates phosphatidylinositol 4-phosphate [PI(4)P] to generate phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	258-267	phosphatidylinositol-4-phosphate 5-kinase type 1 beta	Homo sapiens	0-7
23552101-7	2013	PIP5K1B encodes phosphatidylinositol 4-phosphate 5-kinase beta type I (pip5k1beta), an enzyme functionally linked to actin cytoskeleton dynamics that phosphorylates phosphatidylinositol 4-phosphate [PI(4)P] to generate phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	258-267	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	71-81
23552101-8	2013	Accordingly, loss of pip5k1beta function in FRDA cells was accompanied by decreased PI(4,5)P2 levels and was shown instrumental for destabilization of the actin network and delayed cell spreading.	pi(4,5)p2	84-93	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	21-31
23552101-8	2013	Accordingly, loss of pip5k1beta function in FRDA cells was accompanied by decreased PI(4,5)P2 levels and was shown instrumental for destabilization of the actin network and delayed cell spreading.	pi(4,5)p2	84-93	frataxin	Homo sapiens	44-48
23843517-6	2013	Maximal pharmacological TRPV1 stimulation led to a robust decrease of both PI(4,5)P2 and its precursor PI(4)P in sensory neurons.	pi(4,5)p2	75-84	transient receptor potential cation channel, subfamily V, member 1	Mus musculus	24-29
23843517-7	2013	Attenuating the decrease of either lipid significantly reduced desensitization, and simultaneous reduction of PI(4,5)P2 and PI(4)P independently of PLC inhibited TRPV1.	pi(4,5)p2	110-119	transient receptor potential cation channel, subfamily V, member 1	Mus musculus	162-167
23853710-4	2013	PI(4,5)P2 levels at the apical membrane regulate Dia localization in both the MDCK cyst model and in Drosophila tubular epithelia.	pi(4,5)p2	0-9	diaphanous	Drosophila melanogaster	49-52
23853710-5	2013	An N-terminal basic domain of Dia is crucial for apical localization, implying direct binding to PI(4,5)P2.	pi(4,5)p2	97-106	diaphanous	Drosophila melanogaster	30-33
23838183-5	2013	Furthermore, we showed that the Alzheimer"s disease-linked protein presenilin-2 and the channel protein ORAI2 prevented overload of ER Ca(2+) and that feedback from Ca(2+) to phosphatidylinositol 4-kinase and PLCdelta (phospholipase Cdelta) may regulate the abundance of the plasma membrane lipid PI(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to control Ca(2+) extrusion.	pi(4,5)p2	297-306	presenilin 2	Homo sapiens	67-79
23838183-5	2013	Furthermore, we showed that the Alzheimer"s disease-linked protein presenilin-2 and the channel protein ORAI2 prevented overload of ER Ca(2+) and that feedback from Ca(2+) to phosphatidylinositol 4-kinase and PLCdelta (phospholipase Cdelta) may regulate the abundance of the plasma membrane lipid PI(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to control Ca(2+) extrusion.	pi(4,5)p2	297-306	ORAI calcium release-activated calcium modulator 2	Homo sapiens	104-109
23908690-0	2013	Loss of PI(4,5)P2 5-Phosphatase A Contributes to Resistance of Human Melanoma Cells to RAF/MEK Inhibitors.	pi(4,5)p2	8-17	zinc fingers and homeoboxes 2	Homo sapiens	87-90
23908690-0	2013	Loss of PI(4,5)P2 5-Phosphatase A Contributes to Resistance of Human Melanoma Cells to RAF/MEK Inhibitors.	pi(4,5)p2	8-17	mitogen-activated protein kinase kinase 7	Homo sapiens	91-94
23417604-4	2013	A shortage of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] in Galphaq (Q209L) may be responsible for reduced TRPC4 activity.	pi(4,5)p2	53-62	transient receptor potential cation channel subfamily C member 4	Homo sapiens	114-119
23680464-3	2013	The main kinases involved in the production of PI(4,5)P2 are the type I phosphatidylinositol 4-phosphate 5-kinase (PIP5K) family, which consist of three isozymes, alpha, beta and gamma.	pi(4,5)p2	47-56	phosphoinositide kinase, FYVE type zinc finger containing	Mus musculus	115-120
23564459-1	2013	Kir2.1 channels are uniquely activated by phosphoinositide 4,5-bisphosphate (PI(4,5)P2) and can be inhibited by other phosphoinositides (PIPs).	pi(4,5)p2	77-86	potassium inwardly rectifying channel subfamily J member 2	Homo sapiens	0-6
23564459-6	2013	However, 96-99% of PIP binding localizes to the first cluster, which corresponds to the general PI(4,5)P2 binding location in recent Kir crystal structures.	pi(4,5)p2	96-105	prolactin induced protein	Homo sapiens	19-22
23424177-2	2013	Cargo binding by AP-1 is activated by the small GTPase Arf1, while AP-2 is activated by the phosphoinositide PI(4,5)P2.	pi(4,5)p2	109-118	FosB proto-oncogene, AP-1 transcription factor subunit	Homo sapiens	17-21
23595985-4	2013	We show that the kindlin 3 PH domain has binding affinity for phosphoinositide PI(3,4,5)P3 over PI(4,5)P2.	pi(4,5)p2	96-105	FERM domain containing kindlin 3	Homo sapiens	17-26
23595985-6	2013	A splice variant, kindlin 3-IPRR, has a four-residue insert in the PH domain at a critical site that influences phosphoinositide binding by enhancing binding to PI(4,5)P2 as well as by binding to PI(3,4,5)P3.	pi(4,5)p2	161-170	FERM domain containing kindlin 3	Homo sapiens	18-27
23424177-2	2013	Cargo binding by AP-1 is activated by the small GTPase Arf1, while AP-2 is activated by the phosphoinositide PI(4,5)P2.	pi(4,5)p2	109-118	transcription factor AP-2 alpha	Homo sapiens	67-71
23589871-5	2013	In liposomes containing PI(4,5)P2 as the sole phosphoinositide, actin polymerization requires transducer of Cdc42 activation-1 (toca-1).	pi(4,5)p2	24-33	cell division cycle 42	Homo sapiens	108-113
23434911-0	2013	Low hippocampal PI(4,5)P2 contributes to reduced cognition in old mice as a result of loss of MARCKS.	pi(4,5)p2	16-25	myristoylated alanine rich protein kinase C substrate	Mus musculus	94-100
23434911-5	2013	Consistent with a cause-effect relationship, raising MARCKS levels in the brain of old mice led to increased synaptic membrane clustering of PI(4,5)P2 and to PLCgamma activation.	pi(4,5)p2	141-150	myristoylated alanine rich protein kinase C substrate	Mus musculus	53-59
23392104-3	2013	New evidence, focused on C. elegans RAB-10 in polarized epithelia, points to a key role of RAB-10 in the regulation of endosomal phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2) levels.	pi(4,5)p2	168-177	Ras-related protein Rab-10	Caenorhabditis elegans	91-97
23392104-5	2013	Part of the effect of RAB-10 on endosomal PI(4,5)P2 is through its newly identified effector CNT-1, a predicted GTPase activating protein (GAP) of the small GTPase ARF-6/Arf6.	pi(4,5)p2	42-51	Ras-related protein Rab-10	Caenorhabditis elegans	22-28
23392104-5	2013	Part of the effect of RAB-10 on endosomal PI(4,5)P2 is through its newly identified effector CNT-1, a predicted GTPase activating protein (GAP) of the small GTPase ARF-6/Arf6.	pi(4,5)p2	42-51	Ankyrin_rpt-contain_dom domain-containing protein;Truncated ctn-1	Caenorhabditis elegans	93-98
23392104-5	2013	Part of the effect of RAB-10 on endosomal PI(4,5)P2 is through its newly identified effector CNT-1, a predicted GTPase activating protein (GAP) of the small GTPase ARF-6/Arf6.	pi(4,5)p2	42-51	ADP-ribosylation factor 6	Caenorhabditis elegans	164-169
23392104-5	2013	Part of the effect of RAB-10 on endosomal PI(4,5)P2 is through its newly identified effector CNT-1, a predicted GTPase activating protein (GAP) of the small GTPase ARF-6/Arf6.	pi(4,5)p2	42-51	ADP-ribosylation factor 6	Caenorhabditis elegans	170-174
23392104-7	2013	In C. elegans we found that RAB-10, CNT-1 and ARF-6 are present on the same endosomes, that RAB-10 recruits CNT-1 to endosomes, and that loss of CNT-1 or RAB-10 leads to overaccumulation of endosomal PI(4,5)P2, presumably via hyperactivation of endosomal ARF-6.	pi(4,5)p2	200-209	Ras-related protein Rab-10	Caenorhabditis elegans	92-98
23392104-7	2013	In C. elegans we found that RAB-10, CNT-1 and ARF-6 are present on the same endosomes, that RAB-10 recruits CNT-1 to endosomes, and that loss of CNT-1 or RAB-10 leads to overaccumulation of endosomal PI(4,5)P2, presumably via hyperactivation of endosomal ARF-6.	pi(4,5)p2	200-209	Ras-related protein Rab-10	Caenorhabditis elegans	92-98
23392104-8	2013	In turn this leads to over-recruitment of PI(4,5)P2-dependent membrane-bending proteins RME-1/Ehd and SDPN-1/Syndapin/PACSIN.	pi(4,5)p2	42-51	Receptor Mediated Endocytosis	Caenorhabditis elegans	88-93
23392104-8	2013	In turn this leads to over-recruitment of PI(4,5)P2-dependent membrane-bending proteins RME-1/Ehd and SDPN-1/Syndapin/PACSIN.	pi(4,5)p2	42-51	SynDaPiN (synaptic dynamin binding protein) homolog	Caenorhabditis elegans	102-108
23392104-9	2013	Conversely, in arf-6 mutants, endosomal PI(4,5)P2 levels were reduced and endosomal recruitment of RME-1 and SDPN-1 failed.	pi(4,5)p2	40-49	ADP-ribosylation factor 6	Caenorhabditis elegans	15-20
23589871-5	2013	In liposomes containing PI(4,5)P2 as the sole phosphoinositide, actin polymerization requires transducer of Cdc42 activation-1 (toca-1).	pi(4,5)p2	24-33	formin binding protein 1 like	Homo sapiens	128-134
23497128-8	2013	RESULTS: Initially, confocal microscopic analysis demonstrated that PI(4,5)P2 not only co-localized with NS3, but it also co-localized with VP5, one of the outer capsid proteins of BTV.	pi(4,5)p2	68-77	KRAS proto-oncogene, GTPase	Homo sapiens	105-108
22595022-0	2012	Preso regulation of dendritic outgrowth through PI(4,5)P2-dependent PDZ interaction with betaPix.	pi(4,5)p2	48-57	FERM and PDZ domain containing 4	Homo sapiens	0-5
23442912-1	2013	Phosphatase and tensin-homolog deleted on chromosome 10 (PTEN) is a tumor-suppressor protein that regulates phosphatidylinositol 3-kinase (PI3-K) signaling by binding to the plasma membrane and hydrolyzing the 3" phosphate from phosphatidylinositol (3,4,5)-trisphosphate (PI(3,4,5)P3) to form phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2).	pi(4,5)p2	334-343	phosphatase and tensin homolog	Homo sapiens	57-61
23442912-1	2013	Phosphatase and tensin-homolog deleted on chromosome 10 (PTEN) is a tumor-suppressor protein that regulates phosphatidylinositol 3-kinase (PI3-K) signaling by binding to the plasma membrane and hydrolyzing the 3" phosphate from phosphatidylinositol (3,4,5)-trisphosphate (PI(3,4,5)P3) to form phosphatidylinositol (4,5)-bisphosphate (PI(4,5)P2).	pi(4,5)p2	334-343	phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta	Homo sapiens	108-137
23443536-0	2013	PI(4,5)P2 5-phosphatase A regulates PI3K/Akt signalling and has a tumour suppressive role in human melanoma.	pi(4,5)p2	0-9	AKT serine/threonine kinase 1	Homo sapiens	41-44
23405144-6	2013	The inhibition of the phosphatidylinositol 4,5-bisphospahte [PI(4,5)P2]-PLC pathway, using neomycin and U73122, suppressed the phot2-mediated chloroplast accumulation and avoidance responses, without affecting movement responses controlled by phot1.	pi(4,5)p2	61-70	phospholipase C1	Arabidopsis thaliana	72-75
23405144-11	2013	These results demonstrate the importance of PIs in chloroplast movements, with the PI(4,5)P2-PLC pathway involved in phot2 signaling while PI3K and PI4K are required for the phot1- and phot2-induced accumulation response.	pi(4,5)p2	83-92	phospholipase C1	Arabidopsis thaliana	93-96
22869721-7	2012	Consistent with negative regulation of ARF-6 by RAB-10 and CNT-1, we found overaccumulation of endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in cnt-1 and rab-10 mutants and reduced endosomal PI(4,5)P2 levels in arf-6 mutants.	pi(4,5)p2	144-153	ADP ribosylation factor 6	Homo sapiens	39-44
22869721-7	2012	Consistent with negative regulation of ARF-6 by RAB-10 and CNT-1, we found overaccumulation of endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in cnt-1 and rab-10 mutants and reduced endosomal PI(4,5)P2 levels in arf-6 mutants.	pi(4,5)p2	144-153	solute carrier family 28 member 1	Homo sapiens	59-64
22869721-7	2012	Consistent with negative regulation of ARF-6 by RAB-10 and CNT-1, we found overaccumulation of endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in cnt-1 and rab-10 mutants and reduced endosomal PI(4,5)P2 levels in arf-6 mutants.	pi(4,5)p2	144-153	solute carrier family 28 member 1	Homo sapiens	158-163
22869721-7	2012	Consistent with negative regulation of ARF-6 by RAB-10 and CNT-1, we found overaccumulation of endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in cnt-1 and rab-10 mutants and reduced endosomal PI(4,5)P2 levels in arf-6 mutants.	pi(4,5)p2	144-153	RAB10, member RAS oncogene family	Homo sapiens	168-174
22869721-7	2012	Consistent with negative regulation of ARF-6 by RAB-10 and CNT-1, we found overaccumulation of endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in cnt-1 and rab-10 mutants and reduced endosomal PI(4,5)P2 levels in arf-6 mutants.	pi(4,5)p2	144-153	ADP ribosylation factor 6	Homo sapiens	225-230
22869721-7	2012	Consistent with negative regulation of ARF-6 by RAB-10 and CNT-1, we found overaccumulation of endosomal phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in cnt-1 and rab-10 mutants and reduced endosomal PI(4,5)P2 levels in arf-6 mutants.	pi(4,5)p2	205-214	ADP ribosylation factor 6	Homo sapiens	39-44
22869721-9	2012	Our studies identify a RAB-10-to-ARF-6 regulatory loop required to regulate endosomal PI(4,5)P2, a key phosphoinositide in membrane traffic.	pi(4,5)p2	86-95	RAB10, member RAS oncogene family	Homo sapiens	23-29
22869721-9	2012	Our studies identify a RAB-10-to-ARF-6 regulatory loop required to regulate endosomal PI(4,5)P2, a key phosphoinositide in membrane traffic.	pi(4,5)p2	86-95	ADP ribosylation factor 6	Homo sapiens	33-38
22730382-0	2012	Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2)-dependent oligomerization of fibroblast growth factor 2 (FGF2) triggers the formation of a lipidic membrane pore implicated in unconventional secretion.	pi(4,5)p2	39-48	fibroblast growth factor 2	Homo sapiens	79-105
22730382-0	2012	Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2)-dependent oligomerization of fibroblast growth factor 2 (FGF2) triggers the formation of a lipidic membrane pore implicated in unconventional secretion.	pi(4,5)p2	39-48	fibroblast growth factor 2	Homo sapiens	107-111
22595022-0	2012	Preso regulation of dendritic outgrowth through PI(4,5)P2-dependent PDZ interaction with betaPix.	pi(4,5)p2	48-57	Rho guanine nucleotide exchange factor 7	Homo sapiens	89-96
21762810-0	2011	Sprouty2 regulates PI(4,5)P2/Ca2+ signaling and HIV-1 Gag release.	pi(4,5)p2	19-28	sprouty RTK signaling antagonist 2	Homo sapiens	0-8
22183723-4	2012	Pharmacological and molecular interventions suggest that depletion of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is most likely the critical event for VMI in all three channels.When the PLC catalytic signal was vigorously activated through overexpression of the muscarinic type-I receptor (M1R), the inactivation of macroscopic TRPC currents was greatly accelerated in the same rank order as the VMI, and VMI of these currents was attenuated or lost.	pi(4,5)p2	109-118	cholinergic receptor muscarinic 1	Homo sapiens	269-300
22183723-7	2012	These results demonstrate that TRPC3/C6/C7 channels are differentially regulated by depletion of PI(4,5)P2, and that the bimodal signal produced by PLC activation controls these channels in a self-limiting manner.	pi(4,5)p2	97-106	transient receptor potential cation channel subfamily C member 3	Homo sapiens	31-36
22243752-0	2012	NMDA receptor-mediated PIP5K activation to produce PI(4,5)P2 is essential for AMPA receptor endocytosis during LTD. NMDA receptor activation leads to clathrin-dependent endocytosis of postsynaptic AMPA receptors.	pi(4,5)p2	51-60	phosphoinositide kinase, FYVE-type zinc finger containing	Homo sapiens	23-28
21813603-2	2011	HIV-1 MA has a phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] binding pocket; depletion of this phospholipid from the plasma membrane compromises Gag membrane association and virus budding.	pi(4,5)p2	56-65	Pr55(Gag)	Human immunodeficiency virus 1	151-154
21813603-3	2011	We used multiple methods to examine the possible role of PI(4,5)P2 in Gag-membrane interaction of the alpharetrovirus Rous sarcoma virus (RSV).	pi(4,5)p2	57-66	Pr76 polyprotein precursor	Rous sarcoma virus	70-73
21538148-1	2011	Phosphatidylinositol 4-phosphate 5-kinase (PI(4)P5K) is a type I lipid kinase that generates the lipid second messenger phospholipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and functions downstream of RhoA in actin organization.	pi(4,5)p2	172-181	phosphatidylinositol-5-phosphate 4-kinase type 2 gamma	Homo sapiens	0-51
21538148-1	2011	Phosphatidylinositol 4-phosphate 5-kinase (PI(4)P5K) is a type I lipid kinase that generates the lipid second messenger phospholipid phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and functions downstream of RhoA in actin organization.	pi(4,5)p2	172-181	ras homolog family member A	Homo sapiens	211-215
20886905-3	2010	A combination of in vivo, in vitro, and structural studies have shown that Gag targeting and assembly on the PM are mediated by specific interactions between the myristoylated matrix [myr(+)MA] domain of Gag and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	251-260	Pr55(Gag)	Human immunodeficiency virus 1	75-78
21861907-5	2011	Additionally, activation of TRPV1 by LAs involves PLC and PI(4,5)P2-signalling.	pi(4,5)p2	58-67	transient receptor potential cation channel subfamily V member 1	Homo sapiens	28-33
21316588-0	2011	Synaptojanin 1-mediated PI(4,5)P2 hydrolysis is modulated by membrane curvature and facilitates membrane fission.	pi(4,5)p2	24-33	synaptojanin 1	Homo sapiens	0-14
21316588-3	2011	Using cell-free assays with liposomes of varying diameters, we show that the major synaptic phosphoinositide phosphatase, synaptojanin 1 (Synj1), acts with membrane curvature generators/sensors, such as the BAR protein endophilin, to preferentially remove PI(4,5)P2 from curved membranes as opposed to relatively flat ones.	pi(4,5)p2	256-265	synaptojanin 1	Homo sapiens	122-136
21316588-3	2011	Using cell-free assays with liposomes of varying diameters, we show that the major synaptic phosphoinositide phosphatase, synaptojanin 1 (Synj1), acts with membrane curvature generators/sensors, such as the BAR protein endophilin, to preferentially remove PI(4,5)P2 from curved membranes as opposed to relatively flat ones.	pi(4,5)p2	256-265	synaptojanin 1	Homo sapiens	138-143
21316588-5	2011	Our study raises the possibility that geometry-based mechanisms may contribute to spatially restricting PI(4,5)P2 elimination during membrane internalization and suggests that the PI(4,5)P2-to-PI4P conversion achieved by Synj1 at sites of high curvature may cooperate with dynamin to achieve membrane fission.	pi(4,5)p2	104-113	synaptojanin 1	Homo sapiens	221-226
21316588-5	2011	Our study raises the possibility that geometry-based mechanisms may contribute to spatially restricting PI(4,5)P2 elimination during membrane internalization and suggests that the PI(4,5)P2-to-PI4P conversion achieved by Synj1 at sites of high curvature may cooperate with dynamin to achieve membrane fission.	pi(4,5)p2	180-189	synaptojanin 1	Homo sapiens	221-226
21658948-0	2011	The inositol 5-phosphatase dOCRL controls PI(4,5)P2 homeostasis and is necessary for cytokinesis.	pi(4,5)p2	42-51	Oculocerebrorenal syndrome of Lowe	Drosophila melanogaster	4-26
21658948-0	2011	The inositol 5-phosphatase dOCRL controls PI(4,5)P2 homeostasis and is necessary for cytokinesis.	pi(4,5)p2	42-51	Oculocerebrorenal syndrome of Lowe	Drosophila melanogaster	27-32
21658948-7	2011	We demonstrate that dOCRL is associated with endosomes and that it dephosphorylates PI(4,5)P2 on internal membranes to restrict this phosphoinositide at the plasma membrane and thereby regulates cleavage furrow formation and ingression.	pi(4,5)p2	84-93	Oculocerebrorenal syndrome of Lowe	Drosophila melanogaster	20-25
21321070-0	2011	Voltage- and temperature-dependent activation of TRPV3 channels is potentiated by receptor-mediated PI(4,5)P2 hydrolysis.	pi(4,5)p2	100-109	transient receptor potential cation channel subfamily V member 3	Homo sapiens	49-54
20935082-7	2011	Because AGs are known to reduce PI(4,5)P2 availability by sequestration, inhibition of KCNQ4 may be involved in the action of AGs on OHCs.	pi(4,5)p2	32-41	potassium voltage-gated channel subfamily Q member 4	Homo sapiens	87-92
20886905-3	2010	A combination of in vivo, in vitro, and structural studies have shown that Gag targeting and assembly on the PM are mediated by specific interactions between the myristoylated matrix [myr(+)MA] domain of Gag and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	251-260	Pr55(Gag)	Human immunodeficiency virus 1	204-207
20496925-3	2010	Recent studies indicated that d-myo-phosphatidylinositol (PI) 4,5-bisphosphate (PI(4,5)P2) regulates Pr55(Gag) localization and assembly.	pi(4,5)p2	80-89	Pr55(Gag)	Human immunodeficiency virus 1	106-109
19632983-8	2009	Together, our findings lend support to the view that synaptotagmin functions by binding in a trans configuration whereby the C2A domain binds to the synaptic vesicle and the C2B binds to the PI(4,5)P2-enriched plasma membrane.	pi(4,5)p2	191-200	secretoglobin family 2B member 3, pseudogene	Homo sapiens	174-177
19158393-2	2009	Phosphatidylinositol-4-phosphate 5-kinase 1alpha (PIP5K1alpha) is critical in synthesizing phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2].	pi(4,5)p2	130-139	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	50-61
19026719-0	2009	Myelin basic protein co-distributes with other PI(4,5)P2-sequestering proteins in Triton X-100 detergent-resistant membrane microdomains.	pi(4,5)p2	47-56	myelin basic protein	Rattus norvegicus	0-20
19824478-4	2009	In vitro, proteins that sever or depolymerize actin filaments, such as gelsolin, villin, cofilin and profilin, are inactivated by PI(4,5)P2.	pi(4,5)p2	130-139	gelsolin	Homo sapiens	71-79
19824478-4	2009	In vitro, proteins that sever or depolymerize actin filaments, such as gelsolin, villin, cofilin and profilin, are inactivated by PI(4,5)P2.	pi(4,5)p2	130-139	cofilin 1	Homo sapiens	89-96
19158393-5	2009	Knockdown of PIP5K1alpha in human keratinocytes blocked Cao-induced increases in the binding of PI(3,4,5)P3 to PLC-gamma1; PLC-gamma1 activity; levels of PI(4,5)P2, IP3, and Cai; and induction of keratinocyte differentiation markers.	pi(4,5)p2	154-163	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	13-24
19158393-8	2009	These results indicate that after Cao stimulation PIP5K1alpha is recruited by the E-cadherin-catenin complex to the plasma membrane where it provides the substrate PI(4,5)P2 for both PI3K and PLC-gamma1.	pi(4,5)p2	164-173	phosphatidylinositol-4-phosphate 5-kinase type 1 alpha	Homo sapiens	50-61
19158393-8	2009	These results indicate that after Cao stimulation PIP5K1alpha is recruited by the E-cadherin-catenin complex to the plasma membrane where it provides the substrate PI(4,5)P2 for both PI3K and PLC-gamma1.	pi(4,5)p2	164-173	cadherin 1	Homo sapiens	82-92
19158393-8	2009	These results indicate that after Cao stimulation PIP5K1alpha is recruited by the E-cadherin-catenin complex to the plasma membrane where it provides the substrate PI(4,5)P2 for both PI3K and PLC-gamma1.	pi(4,5)p2	164-173	phospholipase C gamma 1	Homo sapiens	192-202