PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 25301938-9 2014 Fourth, differential positioning of 17alpha-hydroxypregnenolone offers a mechanism whereby allosteric binding of cytochrome b5 might selectively enhance the lyase reaction. 17-alpha-Hydroxypregnenolone 36-63 cytochrome b5 type A Homo sapiens 113-126 29710837-5 2018 Molecular dynamics (MD) simulations showed that V366M creates a &ldquo;one-way valve&rdquo; and suggests a mechanism for dual activities of human CYP17A1 where, after the conversion of pregnenolone to 17OH-pregnenolone, the product exits the active site and re-enters for conversion to dehydroepiandrosterone. 17-alpha-Hydroxypregnenolone 209-226 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 154-161 28387522-7 2017 Finally, differences in the hydrogen-bond pattern of the substrates were detected both in the CYP17A1-Cpd I and CYP17A1-POA complexes, with the former found to be more pivotal for the hydroxylation site than the latter, suggesting a possible explanation for the slower conversion of CYP17A1 for 17alpha-hydroxyprogesterone over 17alpha-hydroxypregnenolone. 17-alpha-Hydroxypregnenolone 328-355 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 94-101 28387522-7 2017 Finally, differences in the hydrogen-bond pattern of the substrates were detected both in the CYP17A1-Cpd I and CYP17A1-POA complexes, with the former found to be more pivotal for the hydroxylation site than the latter, suggesting a possible explanation for the slower conversion of CYP17A1 for 17alpha-hydroxyprogesterone over 17alpha-hydroxypregnenolone. 17-alpha-Hydroxypregnenolone 328-355 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 112-119 28387522-7 2017 Finally, differences in the hydrogen-bond pattern of the substrates were detected both in the CYP17A1-Cpd I and CYP17A1-POA complexes, with the former found to be more pivotal for the hydroxylation site than the latter, suggesting a possible explanation for the slower conversion of CYP17A1 for 17alpha-hydroxyprogesterone over 17alpha-hydroxypregnenolone. 17-alpha-Hydroxypregnenolone 328-355 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 112-119 26668369-2 2015 The multifunctional cytochrome P450 17A1 (CYP17A1) first catalyzes the typical hydroxylation of its primary substrate, pregnenolone (PREG) and then also orchestrates a remarkable C17-C20 bond cleavage (lyase) reaction, converting the 17-hydroxypregnenolone initial product to dehydroepiandrosterone, a process representing the first committed step in the biosynthesis of androgens. 17-alpha-Hydroxypregnenolone 234-256 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 20-40 26668369-2 2015 The multifunctional cytochrome P450 17A1 (CYP17A1) first catalyzes the typical hydroxylation of its primary substrate, pregnenolone (PREG) and then also orchestrates a remarkable C17-C20 bond cleavage (lyase) reaction, converting the 17-hydroxypregnenolone initial product to dehydroepiandrosterone, a process representing the first committed step in the biosynthesis of androgens. 17-alpha-Hydroxypregnenolone 234-256 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 42-49 26668369-2 2015 The multifunctional cytochrome P450 17A1 (CYP17A1) first catalyzes the typical hydroxylation of its primary substrate, pregnenolone (PREG) and then also orchestrates a remarkable C17-C20 bond cleavage (lyase) reaction, converting the 17-hydroxypregnenolone initial product to dehydroepiandrosterone, a process representing the first committed step in the biosynthesis of androgens. 17-alpha-Hydroxypregnenolone 234-256 cytokine like 1 Homo sapiens 179-182 22285649-1 2012 The biosynthesis of androgens requires multiple steps and during the conversion of pregnenolone to 17alpha-hydroxypregnenolone and dehydroepiandrosterone (DHEA) by CYP17a1. 17-alpha-Hydroxypregnenolone 99-126 cytochrome P450, family 17, subfamily a, polypeptide 1 Mus musculus 164-171 23620596-8 2013 Significantly, the CYP17A1/b5 interaction is stronger when the hydroxylase substrate pregnenolone is present in the CYP17A1 active site than when the lyase substrate 17alpha-hydroxypregnenolone is in the active site. 17-alpha-Hydroxypregnenolone 166-193 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 19-26 23620596-8 2013 Significantly, the CYP17A1/b5 interaction is stronger when the hydroxylase substrate pregnenolone is present in the CYP17A1 active site than when the lyase substrate 17alpha-hydroxypregnenolone is in the active site. 17-alpha-Hydroxypregnenolone 166-193 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 116-123 22951291-4 2012 The 17,20-lyase activity of CYP17A1 additionally promotes the conversion of 17-OHP and 17alpha-hydroxypregnenolone to androgens in the classical Delta(4) and Delta(5) pathways. 17-alpha-Hydroxypregnenolone 87-114 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 28-35 24671419-6 2014 Notably, titration of b5 to CYP17A1 pregnenolone induced a set of conformational states closely resembling those of CYP17A1 17alpha-hydroxypregnenolone without b5, providing structural evidence consistent with the reported ability of b5 to selectively enhance 17,20-lyase activity. 17-alpha-Hydroxypregnenolone 124-151 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 28-35 24671419-6 2014 Notably, titration of b5 to CYP17A1 pregnenolone induced a set of conformational states closely resembling those of CYP17A1 17alpha-hydroxypregnenolone without b5, providing structural evidence consistent with the reported ability of b5 to selectively enhance 17,20-lyase activity. 17-alpha-Hydroxypregnenolone 124-151 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 116-123 23874725-2 2013 Type 2 HSD3B catalyzes the conversion of pregnenolone, 17alpha-hydroxypregnenolone and dehydroepiandrosterone to progesterone, 17alpha-hydroxyprogesterone and androstenedione in the human adrenal cortex and the gonads but the exact regulation of this enzyme is unknown. 17-alpha-Hydroxypregnenolone 55-82 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 Homo sapiens 7-12 14504283-5 2003 However, mutation E305G lacks 17,20-lyase activity for the conversion of 17alpha-hydroxypregnenolone to DHEA, which is the dominant pathway to C19 steroids catalyzed by human CYP17 (the delta5-steroid pathway). 17-alpha-Hydroxypregnenolone 73-100 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 175-180 15640159-1 2005 The enzyme CYP17 primarily regulates androgen production by mediating four reactions: conversion of pregnenolone and progesterone to 17-hydroxypregnenolone and 17-hydroxyprogesterone, respectively (17alpha-hydroxylase activity), followed by conversion of the 17-hydroxylated steroids to dehydroepiandrosterone and androstenedione, respectively (17,20-lyase activity). 17-alpha-Hydroxypregnenolone 133-155 cytochrome P450 family 17 subfamily A member 1 L homeolog Xenopus laevis 11-16 16103714-8 2005 Finally, 17-OH pregnenolone is converted to DHEA by the 17,20 lyase activity of P450c17. 17-alpha-Hydroxypregnenolone 9-27 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 80-87 21586563-7 2011 Using 17-hydroxypregnenolone as the P450c17 substrate, the Michaelis constant for 17,20 lyase activity using N-27 POR or N-27 POR-G3H6 was 1.92 or 1.89 mum and the maximal velocity was 0.041 or 0.042 pmol steroid per picomole P450 per minute, respectively. 17-alpha-Hydroxypregnenolone 6-28 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 36-43 21586563-7 2011 Using 17-hydroxypregnenolone as the P450c17 substrate, the Michaelis constant for 17,20 lyase activity using N-27 POR or N-27 POR-G3H6 was 1.92 or 1.89 mum and the maximal velocity was 0.041 or 0.042 pmol steroid per picomole P450 per minute, respectively. 17-alpha-Hydroxypregnenolone 6-28 cytochrome p450 oxidoreductase Homo sapiens 114-117 21586563-7 2011 Using 17-hydroxypregnenolone as the P450c17 substrate, the Michaelis constant for 17,20 lyase activity using N-27 POR or N-27 POR-G3H6 was 1.92 or 1.89 mum and the maximal velocity was 0.041 or 0.042 pmol steroid per picomole P450 per minute, respectively. 17-alpha-Hydroxypregnenolone 6-28 cytochrome p450 oxidoreductase Homo sapiens 126-129 21559465-6 2011 Mamld1 knockdown did not influence the concentrations of pregnenolone and progesterone but significantly reduced those of 17-OH pregnenolone, 17-OH progesterone, dehydroepiandrosterone, androstenedione, and testosterone in the culture media. 17-alpha-Hydroxypregnenolone 122-140 mastermind-like domain containing 1 Mus musculus 0-6 21184804-11 2011 SULT2A1 encodes the enzyme sulfotransferase 2A1 which is responsible for the sulfonation of deoxycorticosterone and dehydroepiandrosterone as well as pregnenolone and 17-hydroxypregnenolone in human adrenal glands. 17-alpha-Hydroxypregnenolone 167-189 sulfotransferase family 2A member 1 Homo sapiens 0-7 16595705-7 2006 The effect of substrate on the redox potential for each P450c17 was measured in the presence of pregnenolone, progesterone, 17alpha-hydroxypregnenolone and 17alpha-hydroxyprogesterone. 17-alpha-Hydroxypregnenolone 124-151 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 56-63 12631293-6 2003 Our results showed that P450c17 possesses a 16-ene-synthase activity able to transform pregnenolone into 5,16-androstadien-3 beta-ol, without the formation of the precursor 17-hydroxypregnenolone. 17-alpha-Hydroxypregnenolone 173-195 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 24-31 12693981-4 2003 Highly purified cytochromes P45017alpha were used for determination of enzyme activity and specificity in relation to progesterone, pregnenolone, 17alpha-hydroxyprogesterone, and 17alpha-hydroxypregnenolone with registration of the kinetics of reaction product formation using HPLC. 17-alpha-Hydroxypregnenolone 179-206 cytochrome P450, family 17, subfamily a, polypeptide 1 Rattus norvegicus 28-39 12464253-4 2003 Ovine CYP17 expressed in HEK 293 cells converts progesterone to 17-hydroxyprogesterone and pregnenolone to dehydroepiandrosterone via 17-hydroxypregnenolone. 17-alpha-Hydroxypregnenolone 134-156 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 6-11 11867265-1 2002 The 17 alpha-hydroxylase and 17,20-lyase activities of P450c17 lead to the production of 17 alpha-hydroxypregnenolone (17 alpha-OH-Preg) and dehydroepiandrosterone (DHEA), respectively, in different tissues. 17-alpha-Hydroxypregnenolone 89-117 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 55-62 12423360-1 2002 Human cytochrome P450 17alpha-hydroxylase (CYP17) catalyses not only the 17alpha-hydroxlation of pregnenolone and progesterone and the C17,20-side chain cleavage (lyase) of 17alpha-hydroxypregnenolone, necessary for the biosynthesis of C21-glucocorticoids and C19-androgens, but also catalyses the 16alpha-hydroxylation of progesterone. 17-alpha-Hydroxypregnenolone 173-200 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 43-48 12573809-6 2002 Finally, 17-OH pregnenolone is converted to DHEA by the 17,20 lyase activity of P450c17. 17-alpha-Hydroxypregnenolone 9-27 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 80-87 11600513-9 2001 Furthermore, those girls with a low glucose/insulin ratio (<7) had higher body mass index, fasting insulin, free T, and ACTH-stimulated 17-hydroxypregnenolone and lower fasting IGF-binding protein-1 and SHBG than those girls with a glucose/insulin ratio greater than 7. 17-alpha-Hydroxypregnenolone 139-161 insulin Homo sapiens 44-51 10973654-2 2000 DESIGN: Fourteen adult females whose ACTH-stimulated 17-hydroxypregnenolone (17OH-PREG) levels were elevated (>/= 2.3 SD). 17-alpha-Hydroxypregnenolone 53-75 proopiomelanocortin Homo sapiens 37-41 10651755-9 2000 The highest ACTH-stimulated 17-hydroxypregnenolone levels, 147, 339 and 351 nmol/l, were found in those patients with mutations in the HSD3B2 gene. 17-alpha-Hydroxypregnenolone 28-50 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 Homo sapiens 135-141 9582502-10 1998 Furthermore, mIL-1 alpha (10 ng/ml) decreased conversion of (22R)-hydroxycholesterol to testosterone while the conversion of pregnenolone, 17-hydroxypregnenolone, dehydroepiandrosterone and androstenedione to testosterone were not affected. 17-alpha-Hydroxypregnenolone 139-161 interleukin 1 alpha Mus musculus 13-24 10406484-1 1999 Polyclonal antiserum against 3beta,17alpha-dihydroxypregn-5-en-20-one-19-O-(carboxymethyl )-oxime bovine serum albumin (17alpha-hydroxypregnenolone-19-CMO:BSA), was raised in rabbits. 17-alpha-Hydroxypregnenolone 120-147 albumin Oryctolagus cuniculus 105-118 9888582-1 1998 Human P450c17 catalyzes the 17alpha-hydroxylation of pregnenolone to 17OH pregnenolone and of progesterone to 17alpha-OH progesterone; the same P450c17 enzyme also catalyzes 17,20 lyase activity on the same active site, converting 17OH-pregnenolone to DHEA. 17-alpha-Hydroxypregnenolone 231-248 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 6-13 9888582-1 1998 Human P450c17 catalyzes the 17alpha-hydroxylation of pregnenolone to 17OH pregnenolone and of progesterone to 17alpha-OH progesterone; the same P450c17 enzyme also catalyzes 17,20 lyase activity on the same active site, converting 17OH-pregnenolone to DHEA. 17-alpha-Hydroxypregnenolone 231-248 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 144-151 9890062-8 1998 Finally, 17-OH pregnenolone is converted to DHEA by the 17,20 lyase activity of P450c17. 17-alpha-Hydroxypregnenolone 9-27 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 80-87 9460762-5 1998 By using the pulse-chase technique, TTN was found to stimulate the conversion of [3H]pregnenolone into various steroids, including 17-hydroxypregnenolone, 5 alpha-dihydrotestosterone and 17-hydroxyprogesterone, in a dose-dependent manner. 17-alpha-Hydroxypregnenolone 131-153 titin Homo sapiens 36-39 9281894-6 1997 EGF has also got a suppressive effect on 17 a-hydroxylase enzyme; a key enzyme which is necessary for the hydroxylation of pregnenolone and progesterone in the ovary Hydroxy pregnenolone is a precursor of androstendione, while 17 a-hydroxyprogesterone is a precursor of testosterone. 17-alpha-Hydroxypregnenolone 166-186 epidermal growth factor Homo sapiens 0-3 8751729-7 1996 MAIN OUTCOME MEASURES: Basal E2 and androgen levels as well as dexamethasone-suppressed, ACTH-stimulated 17 alpha-hydroxyprogesterone, 17 alpha-hydroxypregnenolone, and androgen levels before and after ovarian suppression. 17-alpha-Hydroxypregnenolone 135-163 proopiomelanocortin Homo sapiens 89-93 8404664-6 1993 ANF, BNP, and CNP stimulated the production of intermediate precursors of testosterone biosynthesis, which included progesterone, 17 alpha-hydroxy progesterone, androstenedione, pregnenolone, 17 alpha-hydroxy pregnenolone, and dehydroepiandrosterone sulfate. 17-alpha-Hydroxypregnenolone 192-221 natriuretic peptide type A Mus musculus 0-3 7581961-1 1995 Exaggerated adrenal response (ExAR), i.e. hypersecretion of both 17-hydroxypregnenolone (170HPreg) and 17-hydroxyprogesterone(17OHP) in response to adrenocorticotropic hormone (ACTH) stimulation, is frequently found in women with polycystic ovary (PCO) syndrome who had precocious adrenarche. 17-alpha-Hydroxypregnenolone 65-87 proopiomelanocortin Homo sapiens 148-175 7557440-8 1995 Transient expression of the chicken ORF in COS-7 cells demonstrated coupled dehydrogenase and isomerase activities of 3 beta-HSD on three different substrates; pregnenolone, 17 alpha-hydroxypregnenolone and dehydroepiandrosterone. 17-alpha-Hydroxypregnenolone 174-202 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Homo sapiens 118-128 8948449-13 1995 17 alpha-Hydroxypregnenolone was, however, cleaved into the corresponding androgen but only in the presence of cytochrome b5. 17-alpha-Hydroxypregnenolone 0-28 cytochrome b5 type A Homo sapiens 111-124 8226877-4 1993 The 3 beta-HST had an apparent molecular mass of 33 kDa and utilized pregnenolone, 17-hydroxypregnenolone, and dehydroepiandrosterone as substrates. 17-alpha-Hydroxypregnenolone 83-105 fibroblast growth factor 4 Rattus norvegicus 11-14 8404664-6 1993 ANF, BNP, and CNP stimulated the production of intermediate precursors of testosterone biosynthesis, which included progesterone, 17 alpha-hydroxy progesterone, androstenedione, pregnenolone, 17 alpha-hydroxy pregnenolone, and dehydroepiandrosterone sulfate. 17-alpha-Hydroxypregnenolone 192-221 natriuretic peptide type B Mus musculus 5-8 8404664-6 1993 ANF, BNP, and CNP stimulated the production of intermediate precursors of testosterone biosynthesis, which included progesterone, 17 alpha-hydroxy progesterone, androstenedione, pregnenolone, 17 alpha-hydroxy pregnenolone, and dehydroepiandrosterone sulfate. 17-alpha-Hydroxypregnenolone 192-221 natriuretic peptide type C Mus musculus 14-17 1730815-8 1992 Similarly, insulin treatment resulted in a greater than 100% rise in the difference from baseline in the serum molar ratio of 17 alpha-hydroxypregnenolone to dehydroepiandrosterone 30-60 min after ACTH (P less than 0.004), whereas no change in this difference was observed after the saline infusion (P = 0.71). 17-alpha-Hydroxypregnenolone 126-154 insulin Homo sapiens 11-18 8235158-10 1993 It is concluded that 3 beta HSD is frequent in hirsute women and that its diagnosis requires the determination of ACTH stimulated 17 hydroxypregnenolone values and 17 hydroxypregnenolone/17 hydroxyprogesterone ratio. 17-alpha-Hydroxypregnenolone 164-186 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Homo sapiens 21-31 1438282-6 1992 Addition of purified rat liver cytochrome b5 stimulated the 17,20-lyase reaction for the conversion of 17-hydroxypregnenolone to dehydroepiandrosterone, and addition of purified rat NADPH-cytochrome P450 reductase enhanced the formation of omega--1 metabolites from lauric and arachidonic acids. 17-alpha-Hydroxypregnenolone 103-125 cytochrome b5 type A Rattus norvegicus 31-44 8396144-6 1993 The expected enzymatic activities of human P450c17 were reconstituted by addition of purified rat liver NADPH-cytochrome P450 reductase, giving turnover numbers of 8.0 nmol/min/nmol P450 for pregnenolone, 6.5 nmol/min/nmol P450 for progesterone, 0.06 nmol/min/nmol P450 for 17 alpha-hydroxypregnenolone, and no detectable activity for 17 alpha-hydroxyprogesterone. 17-alpha-Hydroxypregnenolone 274-302 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 43-50 8396144-6 1993 The expected enzymatic activities of human P450c17 were reconstituted by addition of purified rat liver NADPH-cytochrome P450 reductase, giving turnover numbers of 8.0 nmol/min/nmol P450 for pregnenolone, 6.5 nmol/min/nmol P450 for progesterone, 0.06 nmol/min/nmol P450 for 17 alpha-hydroxypregnenolone, and no detectable activity for 17 alpha-hydroxyprogesterone. 17-alpha-Hydroxypregnenolone 274-302 cytochrome p450 oxidoreductase Rattus norvegicus 104-135 8396144-6 1993 The expected enzymatic activities of human P450c17 were reconstituted by addition of purified rat liver NADPH-cytochrome P450 reductase, giving turnover numbers of 8.0 nmol/min/nmol P450 for pregnenolone, 6.5 nmol/min/nmol P450 for progesterone, 0.06 nmol/min/nmol P450 for 17 alpha-hydroxypregnenolone, and no detectable activity for 17 alpha-hydroxyprogesterone. 17-alpha-Hydroxypregnenolone 274-302 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 43-47 8396144-6 1993 The expected enzymatic activities of human P450c17 were reconstituted by addition of purified rat liver NADPH-cytochrome P450 reductase, giving turnover numbers of 8.0 nmol/min/nmol P450 for pregnenolone, 6.5 nmol/min/nmol P450 for progesterone, 0.06 nmol/min/nmol P450 for 17 alpha-hydroxypregnenolone, and no detectable activity for 17 alpha-hydroxyprogesterone. 17-alpha-Hydroxypregnenolone 274-302 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 121-125 8396144-6 1993 The expected enzymatic activities of human P450c17 were reconstituted by addition of purified rat liver NADPH-cytochrome P450 reductase, giving turnover numbers of 8.0 nmol/min/nmol P450 for pregnenolone, 6.5 nmol/min/nmol P450 for progesterone, 0.06 nmol/min/nmol P450 for 17 alpha-hydroxypregnenolone, and no detectable activity for 17 alpha-hydroxyprogesterone. 17-alpha-Hydroxypregnenolone 274-302 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 121-125 8456898-11 1993 Patients with an exaggerated dehydroepiandrosterone or 17-hydroxypregnenolone increment had higher circulating dehydroepiandrosterone sulfate levels but similar basal total and free testosterone, sex hormone-binding globulin, luteinizing and follicle-stimulating hormone concentrations, basal or stimulated androstenedione, dehydroepiandrosterone/androstenedione, and 17-hydroxypregnenolone/17-hydroxyprogesterone than their less responsive counterparts. 17-alpha-Hydroxypregnenolone 55-77 sex hormone binding globulin Homo sapiens 196-224 1331012-6 1992 In contrast, the inhibition of testosterone synthesis produced by either IL-2 or INF gamma plus TNF alpha could be reversed by 17 alpha-hydroxypregnenolone and dehydroepiandrosterone, but not by 22-hydroxycholesterol (P < 0.01). 17-alpha-Hydroxypregnenolone 127-155 interleukin 2 Mus musculus 73-77 1331012-6 1992 In contrast, the inhibition of testosterone synthesis produced by either IL-2 or INF gamma plus TNF alpha could be reversed by 17 alpha-hydroxypregnenolone and dehydroepiandrosterone, but not by 22-hydroxycholesterol (P < 0.01). 17-alpha-Hydroxypregnenolone 127-155 tumor necrosis factor Mus musculus 96-105 1730815-9 1992 Again, the insulin-induced change in this steroid ratio was due to a relative increase in precursor (17 alpha-hydroxypregnenolone) and decrease in product (dehydroepiandrosterone) responsiveness to ACTH. 17-alpha-Hydroxypregnenolone 101-129 insulin Homo sapiens 11-18 34483146-10 2022 Rapid ACTH injection resulted in elevations in the deoxycorticosterone, corticosterone, aldosterone, and 17-hydroxypregnenolone levels, but not in the cortisol level. 17-alpha-Hydroxypregnenolone 105-127 proopiomelanocortin Homo sapiens 6-10 34662099-3 2021 An important branch point in human androgen production is catalyzed by cytochrome P450 CYP17A1 and involves an initial Compound I-mediated hydroxylation at the 17-position of either progesterone (PROG) or pregnenolone (PREG) to form 17-hydroxy derivatives, 17OH-PROG and 17OH-PREG, with approximately similar efficiencies. 17-alpha-Hydroxypregnenolone 271-280 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 87-94 1955079-5 1991 The expressed protein was similar in size to 3 beta-HSD present in H540 Leydig tumor cell homogenate and human placental microsomal 3 beta-HSD, as detected by immunoblot analysis, and catalyzed the conversion of pregnenolone to progesterone, 17 alpha-hydroxypregnenolone to 17 alpha-hydroxyprogesterone, and dehydroepiandrosterone to androstenedione. 17-alpha-Hydroxypregnenolone 242-270 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Rattus norvegicus 45-55 1955079-5 1991 The expressed protein was similar in size to 3 beta-HSD present in H540 Leydig tumor cell homogenate and human placental microsomal 3 beta-HSD, as detected by immunoblot analysis, and catalyzed the conversion of pregnenolone to progesterone, 17 alpha-hydroxypregnenolone to 17 alpha-hydroxyprogesterone, and dehydroepiandrosterone to androstenedione. 17-alpha-Hydroxypregnenolone 242-270 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Rattus norvegicus 132-142 1964539-7 1990 A significant increase of serum hormone levels after ACTH administration could be observed for the following hormones: cortisol, 17 alpha-hydroxyprogesterone, 17 alpha-hydroxypregnenolone and dehydroepiandrosterone. 17-alpha-Hydroxypregnenolone 159-187 proopiomelanocortin Homo sapiens 53-57 2167211-13 1990 Furthermore, IL-2 significantly inhibited the conversion of 20-OH-cholesterol, 22-OH-cholesterol, pregnenolone, progesterone, 17 alpha-hydroxypregnenolone, and 17 alpha-hydroxyprogesterone to testosterone but did not alter the conversion of dehydroepiandrosterone and androstenedione to testosterone. 17-alpha-Hydroxypregnenolone 126-154 interleukin 2 Homo sapiens 13-17 35287432-6 2022 First, we report studies of solvent isotope effects for the N202S CYP17A1 mutant in the presence of 17OH-PREG and 17OH-PROG, which suggest that the ferric peroxo species is the predominant catalytically active intermediate in the lyase step. 17-alpha-Hydroxypregnenolone 100-109 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 66-73 6315754-6 1984 The initial product in human cells, before or after induction with ACTH, was 17-hydroxypregnenolone, which was then converted about equally to cortisol (via 17-hydroxyprogesterone and 11-deoxycortisol) and dehydroepiandrosterone sulfate (via dehydroepiandrosterone). 17-alpha-Hydroxypregnenolone 77-99 proopiomelanocortin Homo sapiens 67-71 35366770-5 2022 This P450c17 cytochrome gene is a critical steroidogenesis regulator which performs two distinct activities: 17 alpha-hydroxylase activity (converting pregnenolone to 17-hydroxypregnenolone and progesterone to 17-hydroxyprogesterone, these precursors are further processed to provide glucocorticoids and sex hormones) and 17, 20-lyase activity (which converts 17-hydroxypregnenolone to DHEA). 17-alpha-Hydroxypregnenolone 167-189 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 5-12 35366770-5 2022 This P450c17 cytochrome gene is a critical steroidogenesis regulator which performs two distinct activities: 17 alpha-hydroxylase activity (converting pregnenolone to 17-hydroxypregnenolone and progesterone to 17-hydroxyprogesterone, these precursors are further processed to provide glucocorticoids and sex hormones) and 17, 20-lyase activity (which converts 17-hydroxypregnenolone to DHEA). 17-alpha-Hydroxypregnenolone 360-382 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 5-12 3220046-2 1988 Her baseline and ACTH-stimulated plasma 17-hydroxy pregnenolone, dehydroepiandrosterone and dehydroepiandrosterone sulfate were increased whereas plasma 17-hydroxy progesterone and androstenedione were normal and responded poorly to ACTH. 17-alpha-Hydroxypregnenolone 40-63 proopiomelanocortin Homo sapiens 17-21 6327404-7 1984 Seven of 16 patients (34%) had exaggerated responses of serum DHEA-S and of 17-OH pregnenolone to ACTH stimulation. 17-alpha-Hydroxypregnenolone 76-94 proopiomelanocortin Homo sapiens 98-102 35225789-3 2022 Because Cyp17a1 plays key roles in hydroxylation of pregnenolone and progesterone (P4), and converts 17alpha-hydroxypregnenolone to dehydroepiandrosterone and 17alpha-hydroxyprogesterone to androstenedione, we hypothesize that the unexpected phenotype in cyp17a1-/-;androgen receptor (ar)-/- zebrafish may be mediated through an augmentation of progestin/nuclear progestin receptor (nPgr) signaling. 17-alpha-Hydroxypregnenolone 101-128 cytochrome P450, family 17, subfamily A, polypeptide 1 Danio rerio 8-15 35225789-3 2022 Because Cyp17a1 plays key roles in hydroxylation of pregnenolone and progesterone (P4), and converts 17alpha-hydroxypregnenolone to dehydroepiandrosterone and 17alpha-hydroxyprogesterone to androstenedione, we hypothesize that the unexpected phenotype in cyp17a1-/-;androgen receptor (ar)-/- zebrafish may be mediated through an augmentation of progestin/nuclear progestin receptor (nPgr) signaling. 17-alpha-Hydroxypregnenolone 101-128 androgen receptor Danio rerio 255-283 35225789-3 2022 Because Cyp17a1 plays key roles in hydroxylation of pregnenolone and progesterone (P4), and converts 17alpha-hydroxypregnenolone to dehydroepiandrosterone and 17alpha-hydroxyprogesterone to androstenedione, we hypothesize that the unexpected phenotype in cyp17a1-/-;androgen receptor (ar)-/- zebrafish may be mediated through an augmentation of progestin/nuclear progestin receptor (nPgr) signaling. 17-alpha-Hydroxypregnenolone 101-128 androgen receptor Danio rerio 285-287 2848596-7 1988 One of the steroidogenic enzymatic steps stimulated by ACTH pretreatment was that of 17 alpha-hydroxylase/17, 20-lyase, since conversion of pregnenolone and 17 alpha-hydroxypregnenolone to DHAS was increased in a dose-dependent manner. 17-alpha-Hydroxypregnenolone 157-185 proopiomelanocortin Homo sapiens 55-59 2846083-3 1988 When phospholipase A2 was included, the 5-ene steroids 17-hydroxypregnenolone and dehydroepiandrosterone (DHEA) were formed in greater quantities, and the production of 4-ene steroids was reduced indicating that the conversion of 5-ene steroids to the 4-ene configuration was inhibited by phospholipase A2 treatment. 17-alpha-Hydroxypregnenolone 55-77 phospholipase A2 group IB Rattus norvegicus 5-21 2982902-5 1985 However, since post-dex baseline concentrations of adrenal steroids were lower, the delta to ACTH was significantly greater for the major adrenal secretory products, 17 alpha-hydroxypregnenolone (3 beta, 17 alpha-dihydroxypregn-5-ene-20-one), dehydroepiandrosterone, and cortisol (F). 17-alpha-Hydroxypregnenolone 166-194 proopiomelanocortin Homo sapiens 93-97 6749579-6 1982 Metabolic studies of GnRH-treated cultures revealed that LH-stimulated androsterone and 5 alpha-androstane-3 alpha, 17 beta-diol were decreased by 90%; androstenedione, testosterone and DHEA were decreased by 70%; 17 alpha-hydroxypregnenolone and 17 alpha-hydroxyprogesterone were decreased by 50%; pregnenolone was unchanged; and progesterone was increased 40%. 17-alpha-Hydroxypregnenolone 214-242 gonadotropin releasing hormone 1 Rattus norvegicus 21-25 6291906-9 1982 Studies on the metabolism of steroid hormones revealed that PRL decreased LH-stimulated androsterone and 5 alpha-androstane-3 alpha, 17 beta-diol accumulation by 99%, androstenedione by 94%, testosterone by 90%, dehydroepiandrosterone by more than 80%, pregnenolone and 17 alpha-hydroxyprogesterone by 80%, 17 alpha-hydroxypregnenolone by 84%, and progesterone by 71%. 17-alpha-Hydroxypregnenolone 307-335 prolactin Rattus norvegicus 60-63 641438-3 1978 5-Unsaturated-3beta-HSD was characterized with pregnenolone, 17alpha-hydroxypregnenolone and dehydroepiandrosterone as substrates; oestradiol-17beta and testosterone were used for the investigation of 17beta-HSD activity. 17-alpha-Hydroxypregnenolone 61-88 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Rattus norvegicus 14-23 6274585-5 1981 The levels of cortisol, pregnenolone, 17-hydroxypregnenolone, dehydroepiandrosterone, androstenedione and dihydrotestosterone were significantly suppressed by dexamethasone and stimulated by ACTH in all phases of the cycle, and before and after DMPA administration. 17-alpha-Hydroxypregnenolone 38-60 proopiomelanocortin Homo sapiens 191-195 6257501-5 1981 After 2 days of estradiol treatment, the maximum hCG stimulation of androstenedione, testosterone, 17 alpha-hydroxypregnenolone, and 17 alpha-hydroxyprogesterone production by ovarian cells was inhibited by 90%; pregnenolone production was unchanged, while progesterone production was increased by 30%. 17-alpha-Hydroxypregnenolone 99-127 chorionic gonadotropin subunit beta 5 Homo sapiens 49-52 422695-2 1979 C21 steroids: progesterone, 16 alpha-hydroxyprogesterone, 17 alpha-hydroxyprogesterone, 20 alpha-dihydroprogesterone, delta 5-pregnenolone, delta 5-pregnenolone sulfate, and 17-hydroxy delta 5-pregnenolone. 17-alpha-Hydroxypregnenolone 174-205 TBL1X/Y related 1 Homo sapiens 0-3 190251-0 1977 Plasma pregnenolone and 17-OH-pregnenolone in patients with adrenal tumors, ACTH excess, or idiopathic hirsutism. 17-alpha-Hydroxypregnenolone 24-42 proopiomelanocortin Homo sapiens 76-80 239964-8 1975 The Michaelis constants (Km) for NAD were 50 muM, 33 muM and 14 muM, respectively for the dehydrogenation of pregnenolone, 17alpha-hydroxypregnenolone, androstenediol and dehydroepiandrosterone. 17-alpha-Hydroxypregnenolone 123-150 latexin Homo sapiens 45-48 239964-8 1975 The Michaelis constants (Km) for NAD were 50 muM, 33 muM and 14 muM, respectively for the dehydrogenation of pregnenolone, 17alpha-hydroxypregnenolone, androstenediol and dehydroepiandrosterone. 17-alpha-Hydroxypregnenolone 123-150 latexin Homo sapiens 53-56 239964-8 1975 The Michaelis constants (Km) for NAD were 50 muM, 33 muM and 14 muM, respectively for the dehydrogenation of pregnenolone, 17alpha-hydroxypregnenolone, androstenediol and dehydroepiandrosterone. 17-alpha-Hydroxypregnenolone 123-150 latexin Homo sapiens 53-56 32638280-10 2021 There was also a positive correlation between IL-18 or IL-1beta and 17-alpha-hydroxypregnenolone, 17-alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA), DHEA sulfate (DHEAS), androstenedione, testosterone, dihydrotestosterone, progesterone, corticosterone, 11-deoxycorticosterone, and the ratio of DHEAS/cortisol. 17-alpha-Hydroxypregnenolone 68-96 interleukin 18 Homo sapiens 46-51 178853-8 1975 The conversion of pregnenolone to 17 alpha-hydroxypregnenolone and dehydroepiandrosterone was increased by ACTH. 17-alpha-Hydroxypregnenolone 34-62 proopiomelanocortin Homo sapiens 107-111 32638280-10 2021 There was also a positive correlation between IL-18 or IL-1beta and 17-alpha-hydroxypregnenolone, 17-alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA), DHEA sulfate (DHEAS), androstenedione, testosterone, dihydrotestosterone, progesterone, corticosterone, 11-deoxycorticosterone, and the ratio of DHEAS/cortisol. 17-alpha-Hydroxypregnenolone 68-96 interleukin 1 alpha Homo sapiens 55-63 32215889-11 2020 CYP17A1 activity of p.G478S was 13 and 58% against progesterone and 17-hydroxypregnenolone, respectively. 17-alpha-Hydroxypregnenolone 68-90 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 0-7 31369102-7 2020 CYP17 activity, as assessed by 17-hydroxypregnenolone/pregnenolone ratio, increased by 60% (P = 0.0389). 17-alpha-Hydroxypregnenolone 31-53 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 0-5 32007561-9 2020 The structurally similar 3,11diOH-DHP4 and alfaxalone showed a greater distance between C17 and the heme group compared to the natural substrate, 17alpha-hydroxypregnenolone potentially allowing more orientational freedom and facilitating the conversion of the C11-oxy C21 to C11-oxy C19 steroids. 17-alpha-Hydroxypregnenolone 146-173 cytokine like 1 Homo sapiens 88-91 32007561-9 2020 The structurally similar 3,11diOH-DHP4 and alfaxalone showed a greater distance between C17 and the heme group compared to the natural substrate, 17alpha-hydroxypregnenolone potentially allowing more orientational freedom and facilitating the conversion of the C11-oxy C21 to C11-oxy C19 steroids. 17-alpha-Hydroxypregnenolone 146-173 aldo-keto reductase family 1 member C4 Homo sapiens 261-264 32007561-9 2020 The structurally similar 3,11diOH-DHP4 and alfaxalone showed a greater distance between C17 and the heme group compared to the natural substrate, 17alpha-hydroxypregnenolone potentially allowing more orientational freedom and facilitating the conversion of the C11-oxy C21 to C11-oxy C19 steroids. 17-alpha-Hydroxypregnenolone 146-173 TBL1X/Y related 1 Homo sapiens 269-272 32007561-9 2020 The structurally similar 3,11diOH-DHP4 and alfaxalone showed a greater distance between C17 and the heme group compared to the natural substrate, 17alpha-hydroxypregnenolone potentially allowing more orientational freedom and facilitating the conversion of the C11-oxy C21 to C11-oxy C19 steroids. 17-alpha-Hydroxypregnenolone 146-173 aldo-keto reductase family 1 member C4 Homo sapiens 276-279 30241348-3 2018 Pregnenolone, progesterone, 17-OH-pregnenolone, and 17-OH-progesterone increased C21 steroid (5-pregnan-3,20-dione, 5-pregnan-3,17-diol-20-one, 5-pregnan-3-ol-20-one) formation in the backdoor pathway, and demonstrated a trend of stimulating dihydroepiandrosterone or its precursors in the backdoor pathway in LNCaP and 22Rv1 cells. 17-alpha-Hydroxypregnenolone 28-46 TBL1X/Y related 1 Homo sapiens 81-84 31509771-1 2019 Cytochrome P450 17A1 (CYP17A1) catalyses the 17alpha-hydroxylation and 17,20 lyase reactions to convert pregnenolone to 17alpha-hydroxypregnenolone (17OHP) and subsequently the androgen dehydroepiandrosterone (DHEA). 17-alpha-Hydroxypregnenolone 120-147 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 0-20 31509771-1 2019 Cytochrome P450 17A1 (CYP17A1) catalyses the 17alpha-hydroxylation and 17,20 lyase reactions to convert pregnenolone to 17alpha-hydroxypregnenolone (17OHP) and subsequently the androgen dehydroepiandrosterone (DHEA). 17-alpha-Hydroxypregnenolone 120-147 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 22-29 30229581-6 2018 And the activities of 17alpha-hydroxylase and 17,20-lyase of CYP17A1 were evaluated by measuring the conversion of progesterone to 17alpha-hydroxyprogesterone and of 17alpha-hydroxypregnenolone to dehydroepiandrosterone, respectively. 17-alpha-Hydroxypregnenolone 166-193 cytochrome P450 family 17 subfamily A member 1 Homo sapiens 61-68