PMID-sentid Pub_year Sent_text compound_name comp_offset prot_official_name organism prot_offset 8752124-6 1996 In contrast to CT105, A beta increased LDH release only slightly even at 50 microM but significantly inhibited 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction at submicromolar concentrations. thiazolyl blue 111-171 amyloid beta precursor protein Homo sapiens 22-28 8920943-1 1996 Tumor necrosis factor alpha mediated cell death in L929 cells correlates with a late increase in reduction of the superoxide scavenger 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), suggesting an increase in MTT reduction per viable cell. thiazolyl blue 135-195 tumor necrosis factor Mus musculus 0-27 8917366-3 1996 Furthermore, a cell to cell adhesion analysis by a colorimetric assay using the dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) demonstrated that CBA-CEACD3 is able to bind CEA-producing cells to CD3-expressing cells, suggesting that both arms of CBA-CEACD3 are simultaneously working and can retarget T-cells to the tumor. thiazolyl blue 84-145 CEA cell adhesion molecule 3 Homo sapiens 174-177 8863516-2 1996 A modified 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, in which a yellow redox dye, MTT, is reduced to purple formazan, is very sensitive to the effect of A beta. thiazolyl blue 11-71 amyloid beta precursor protein Homo sapiens 185-191 7536800-5 1995 The IL-2 receptors were found to be functional because the viability and growth activity of LPS-treated microglia, but not untreated controls, increased in response to recombinant mouse IL-2 as determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay and bromodeoxyuridine uptake experiment, respectively. thiazolyl blue 210-270 interleukin 2 Mus musculus 4-8 8603996-6 1996 LPS-mediated damage to endothelial cells was assayed using the MTT tetrazolium assay. thiazolyl blue 63-78 interferon regulatory factor 6 Homo sapiens 0-3 9816099-3 1996 Growth of SCCHN cell lines incubated in the presence of various concentrations of IL-4 was measured in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assays and by cell counts. thiazolyl blue 103-163 interleukin 4 Homo sapiens 82-86 8679222-5 1996 In parallel experiments, TNF alpha, rTNF alpha-p55, and htr-9 induced human TSMC proliferation as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. thiazolyl blue 114-175 tumor necrosis factor Homo sapiens 25-34 8679222-5 1996 In parallel experiments, TNF alpha, rTNF alpha-p55, and htr-9 induced human TSMC proliferation as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. thiazolyl blue 114-175 tumor necrosis factor Rattus norvegicus 36-40 7536800-5 1995 The IL-2 receptors were found to be functional because the viability and growth activity of LPS-treated microglia, but not untreated controls, increased in response to recombinant mouse IL-2 as determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay and bromodeoxyuridine uptake experiment, respectively. thiazolyl blue 210-270 interleukin 2 Mus musculus 186-190 7699497-2 1995 PATIENTS AND METHODS: Tumor necrosis factor (TNF) activity in synovial fluid from 27 patients with temporomandibular disorders (TMDs) was studied using the MTT tetrazolium WEHI 164 clone 13 cell cytotoxicity assay. thiazolyl blue 156-171 tumor necrosis factor Homo sapiens 45-48 7519747-2 1994 Low concentrations of TNF (1-100 U/ml) suppressed the growth of SF-188 assessed by cell count, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, and thymidine incorporation assay, but not that of LN-382. thiazolyl blue 95-156 tumor necrosis factor Homo sapiens 22-25 7866991-3 1995 We examined the cytotoxicity of SPM-D in small and non-small cell lung cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony assays. thiazolyl blue 95-155 myosin heavy chain 7 Homo sapiens 32-37 8013384-6 1994 TGF beta s did not have any effect on cell proliferation, as determined by cell counting; however, at 0.1 ng/ml and higher concentrations, TGF beta s significantly reduced the metabolic activity of stromal cells, as determined by colorimetric 3-(4,5-dimethylthiazol-2-yl)2, 5-diphenyltetrazolium bromide assay (P < 0.05). thiazolyl blue 243-303 transforming growth factor beta 1 Homo sapiens 139-147 1392061-3 1992 The proliferation-stimulating activity of bFGF was determined by measuring the amount of formazan formed by the mitochondrial enzymes from a tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), instead of counting the viable cell numbers or measuring the incorporation of [3H]-thymidine. thiazolyl blue 159-219 fibroblast growth factor 2 Bos taurus 42-46 8402679-6 1993 The effect of increased GST Yc activity on the sensitivity of the transfected clones to various cytotoxic agents was assessed by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell survival assay. thiazolyl blue 139-199 hematopoietic prostaglandin D synthase Rattus norvegicus 24-27 1402552-2 1992 Optimal responsiveness to EGF was obtained in confluent cultures of primary pig thyrocytes cultured with EGF or biological samples containing EGF for either 24 or 48 h. Dehydrogenase activity was determined by measuring the production and then the release of a coloured formazan product produced by reduction of a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide substrate added to the cells. thiazolyl blue 314-374 epidermal growth factor Sus scrofa 26-29 7906263-5 1994 In the present study, the cytotoxic activity of dFUrd in vitro and dFUrd combined with daunorubicin (DNR) was assessed with the (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium) bromide assay in cells with increased P-gp 170 expression versus controls. thiazolyl blue 128-190 phosphoglycolate phosphatase Homo sapiens 221-225 8286523-1 1993 Swine interleukin 2 (IL-2) activity was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay using murine IL-2 dependent cell line (CTLL-2). thiazolyl blue 51-112 interleukin 2 Mus musculus 6-19 8286523-1 1993 Swine interleukin 2 (IL-2) activity was assayed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay using murine IL-2 dependent cell line (CTLL-2). thiazolyl blue 51-112 interleukin 2 Mus musculus 21-25 8515059-4 1993 IL-2 was quantitated by the rate of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) metabolism with the use of a murine CD4+ IL-2-dependent T lymphocyte line, and dose-response effects of each neuropeptide were examined over a broad concentration range (10(-14)-10(-6) M) encompassing that regarded as physiologic. thiazolyl blue 36-96 interleukin 2 Mus musculus 0-4 2403021-6 1990 IL-2 activity was assayed by assessing its ability to support proliferation of selected IL-2-dependent T cell line, measured by colorimetric method which is based on the ability of viable cells to cleave MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide). thiazolyl blue 209-272 interleukin 2 Mus musculus 0-4 1827689-4 1991 We here describe a shorter assay in which IL-2 and IL-3 secretion during MLR was assessed by adding the respective lymphokine-dependent cell lines either to the MLR supernatants or directly to the original MLR cultures, using the colorimetric (3-[4,5 Dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide) (MTT) technique or the 3H-thymidine incorporation assay. thiazolyl blue 244-304 interleukin 2 Homo sapiens 42-46 1827689-4 1991 We here describe a shorter assay in which IL-2 and IL-3 secretion during MLR was assessed by adding the respective lymphokine-dependent cell lines either to the MLR supernatants or directly to the original MLR cultures, using the colorimetric (3-[4,5 Dimethylthiazol-2-yl]-2.5-diphenyltetrazolium bromide) (MTT) technique or the 3H-thymidine incorporation assay. thiazolyl blue 244-304 interleukin 3 Homo sapiens 51-55 2208141-12 1990 BBS (0.5-50 nM) demonstrated a significant dose-dependent growth effect on MC-26 cells in vitro, in terms of [3H]thymidine and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide uptake; these studies indicate that the BBS/GRP binding sites on MC-26 cells may serve as functional receptors and mediate the growth effects of BBS on MC-26 cells. thiazolyl blue 127-187 gastrin releasing peptide Mus musculus 232-235 2403021-6 1990 IL-2 activity was assayed by assessing its ability to support proliferation of selected IL-2-dependent T cell line, measured by colorimetric method which is based on the ability of viable cells to cleave MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide). thiazolyl blue 209-272 interleukin 2 Mus musculus 88-92 33810180-3 2021 The in vitro results indicated a protective effect of C11 (500, 1000, and 2500 ng/mL) on astrocytes under trophic stress conditions in the MTT (3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide) test. thiazolyl blue 144-205 polymerase (RNA) III (DNA directed) polypeptide K Mus musculus 54-57 33778168-5 2021 In addition, BP treatment was found to have a cytoprotective activity against Abeta-induced cell cytotoxicity as shown by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell toxicity assay. thiazolyl blue 126-186 amyloid beta precursor protein Homo sapiens 78-83 24763891-5 2014 The effect of FGF10 and anti-FGF10 on human keratinocyte HaCaT cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). thiazolyl blue 100-160 fibroblast growth factor 10 Homo sapiens 14-19 34539833-5 2021 It was demonstrated that knockdown of miR155HG markedly inhibited OC cell viability, migration and invasion while promoting apoptosis, as indicated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, wound healing, Transwell and western blot assays. thiazolyl blue 151-211 MIR155 host gene Homo sapiens 38-46 34789124-9 2021 In addition, cell viability in the presence of the recombinant GST-UCMA protein was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell adhesion assay. thiazolyl blue 102-162 upper zone of growth plate and cartilage matrix associated Mus musculus 67-71 34783160-6 2022 The effects of LINC01224 on cell proliferation and apoptosis in melanoma were assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), colony formation, and flow cytometry assays. thiazolyl blue 90-150 long intergenic non-protein coding RNA 1224 Homo sapiens 15-24 34624670-3 2021 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the cytotoxic activity of Ir1 and Ir1Lipo on HepG2, SGC-7901, BEL-7402, HeLa, B16, A549 and normal NIH3T3 cells. thiazolyl blue 4-64 immune response 1 Mus musculus 123-126 34624670-3 2021 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the cytotoxic activity of Ir1 and Ir1Lipo on HepG2, SGC-7901, BEL-7402, HeLa, B16, A549 and normal NIH3T3 cells. thiazolyl blue 4-64 immune response 1 Mus musculus 131-134 34761587-4 2021 Further, effects of Keap1 on the cell proliferation and apoptosis were evaluated through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and flow cytometry assay, respectively. thiazolyl blue 94-155 kelch like ECH associated protein 1 Homo sapiens 20-25 34438219-3 2021 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method was used to detect the cytotoxic activity of Ir1, Ir2 and Ir3, the complexes show highly active against B16 cells with IC50 values of 0.3 +- 0.2 muM, 3.7 +- 0.2 muM and 4.6 +- 1.1 muM, respectively. thiazolyl blue 0-60 nischarin Homo sapiens 119-122 34426145-3 2021 The cytotoxicity of Ir1-3 toward cancer cells was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. thiazolyl blue 61-121 nischarin Homo sapiens 20-25 34082012-4 2021 The functions of YBX1, miR-148a-3p and Runx3 were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, wound healing, transwell, flow cytometry, xenograft model and Western blotting analyses. thiazolyl blue 62-122 Y-box binding protein 1 Homo sapiens 17-21 34082012-4 2021 The functions of YBX1, miR-148a-3p and Runx3 were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, colony formation, wound healing, transwell, flow cytometry, xenograft model and Western blotting analyses. thiazolyl blue 62-122 RUNX family transcription factor 3 Homo sapiens 39-44 34281099-5 2021 The viability of HGF cells was determined using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT), neutral red uptake, and trypan blue assay after treatment with different concentrations of CHX and ME (0.3125 to 10 microg/mL). thiazolyl blue 48-110 hepatocyte growth factor Homo sapiens 17-20 34233701-4 2021 CILP function is assessed by cell cycle assay, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry, beta-galactosidase staining, and the detection of ECM-related molecules aggrecan, collagen type I, collagen type II, MMP-3, and MMP-9 expression is accomplished by qRT-PCR. thiazolyl blue 47-107 cartilage intermediate layer protein Homo sapiens 0-4 34157827-2 2021 Materials and Methods: The cytotoxic effects and protective properties of S. verbenaca on human monocytic leukemia cells (THP-1) was studied using thiazolyl blue tetrazolium bromide assay. thiazolyl blue 147-181 GLI family zinc finger 2 Homo sapiens 122-127 35428675-4 2022 si-DUSP8 or dusp8 overexpression vector was transiently transfected, and the effects of si-DUSP8 on apoptosis, cell viability and cell migration of drug-resistant cell lines were investigated by flow cytometry, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) and Transwell assays, and its regulation mechanism finally explored. thiazolyl blue 216-277 dual specificity phosphatase 8 Homo sapiens 3-8 34193694-6 2021 The function role of Rasal2 in NSCLC cells were measured by small interfering RNA (si-RNA), immunostaining, transwell assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 128-188 RAS protein activator like 2 Homo sapiens 21-27 35428675-4 2022 si-DUSP8 or dusp8 overexpression vector was transiently transfected, and the effects of si-DUSP8 on apoptosis, cell viability and cell migration of drug-resistant cell lines were investigated by flow cytometry, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide) and Transwell assays, and its regulation mechanism finally explored. thiazolyl blue 216-277 dual specificity phosphatase 8 Homo sapiens 91-96 35125056-3 2022 Furthermore, after recombinant human PEDF treatment, the PDLF viability and osteogenic differentiation were examined using the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay, alkaline phosphatase (ALP) activity assay, Von Kossa staining, Alizarin red staining, real-time quantitative polymerase chain reaction (qRT-PCR), and immunoblotting. thiazolyl blue 127-188 serpin family F member 1 Homo sapiens 37-41 35267786-4 2022 The resultant elastin"s toxicity was analyzed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium assay and primary skin irritation test. thiazolyl blue 60-120 elastin Bos taurus 14-21 35291911-4 2022 The influence of ARAP1-AS1 on LUAD cell malignant behaviors was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony formation assay, Transwell invasion assay and wound healing assay. thiazolyl blue 77-137 ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 Homo sapiens 17-22 35291911-4 2022 The influence of ARAP1-AS1 on LUAD cell malignant behaviors was evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony formation assay, Transwell invasion assay and wound healing assay. thiazolyl blue 77-137 prostaglandin D2 receptor Homo sapiens 23-26 35045800-7 2022 Overexpression of lncRNA CASC2 decreased the viability of human fibroblast-like synoviocytes (HFLSs) and induced apoptosis, as revealed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry analyses. thiazolyl blue 148-208 cancer susceptibility 2 Homo sapiens 25-30 34338241-5 2022 The antitumour activity of ACPP-p21Ras scFv on A549 cells and H1299 cells in vitro was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, scratch wound healing, plate cloning and apoptosis assays. thiazolyl blue 101-161 immunglobulin heavy chain variable region Homo sapiens 39-43 2558980-2 1989 NAD[P]H + phenazine + O2 was found to reduce nitroblue tetrazolium, iodonitrotetrazolium, and thiazolyl blue in a manner sensitive to agents that dismutase O2-., viz., SOD and Mn(II). thiazolyl blue 94-108 superoxide dismutase 1 Homo sapiens 168-171 3490518-1 1986 Mosmann"s method for measuring the number of viable cells with a tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT), was modified to make it possible to measure a large number of interleukin 2 (IL-2) samples at one time with less labor and more accuracy. thiazolyl blue 83-144 interleukin 2 Mus musculus 214-227 3141500-4 1988 Concentrations of PMA and ionomycin that maximally inhibited proliferation stimulated maximal lymphokine secretion and increased mitochondrial activity as assessed by measurement of cleavage of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium-bromide. thiazolyl blue 194-255 peroneal muscular atrophy Mus musculus 18-21 3490518-1 1986 Mosmann"s method for measuring the number of viable cells with a tetrazolium salt, 3-(4,5-dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide (MTT), was modified to make it possible to measure a large number of interleukin 2 (IL-2) samples at one time with less labor and more accuracy. thiazolyl blue 83-144 interleukin 2 Mus musculus 229-233 33151037-10 2021 Besides, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays proved that the CS-Zn(II) MNs possess brilliant cytocompatibility. thiazolyl blue 13-73 citrate synthase Homo sapiens 103-105 7258187-1 1981 A two-stage procedure using MTT tetrazolium for the demonstration of aminotransferases (GPT and GOT) either singly or together was developed. thiazolyl blue 28-43 glutamic--pyruvic transaminase Homo sapiens 88-91 41650-0 1979 A new assay for diaphorase activity in reagent formulations, based on the reduction of thiazolyl blue. thiazolyl blue 87-101 dihydrolipoamide dehydrogenase Homo sapiens 16-26 41650-2 1979 The dye thiazolyl blue tetrazolium bromide is reduced in the presence of NADH and diaphorase to yield a colored formazan, which as maximum absorbance at 560 nm. thiazolyl blue 8-42 dihydrolipoamide dehydrogenase Homo sapiens 82-92 33676362-5 2021 MiR-524 mimic was employed to investigate the impact of miR-524 on proliferation, migration, and invasion using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and transwell assays. thiazolyl blue 112-173 microRNA 524 Homo sapiens 0-7 33960240-4 2021 The effect of expression of DFF40 under the control of cytomegalovirus (CMV) or survivin promoter on viability of cancerous and normal cells was determined by MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide] assay. thiazolyl blue 164-224 DNA fragmentation factor, beta subunit Mus musculus 28-33 34035814-4 2021 The regulatory mechanism of miR-552 was explored by (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) MTT Assay, and Transwell assays. thiazolyl blue 52-114 microRNA 552 Homo sapiens 28-35 33694172-8 2021 Sub-cytotoxic concentration in the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test for both BV-2 WT and Acox1-/- cell lines was 5.10-1 microg/ml. thiazolyl blue 35-96 acyl-Coenzyme A oxidase 1, palmitoyl Mus musculus 123-128 33327965-4 2020 The molecular mechanisms of miR-4310 expression and its effects on cell proliferation, migration, and invasion were explored using 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide, Transwell chamber, Boyden chamber, and western blot analyses, as well as its effect on tumorigenesis was explored in vivo in nude mice. thiazolyl blue 131-192 microRNA 4310 Homo sapiens 28-36 33841100-9 2021 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and calcium imaging analysis showed that the IRE1alpha inhibitor, 4mu8c, reduced Abeta-induced cytotoxicity. thiazolyl blue 0-60 endoplasmic reticulum to nucleus signaling 1 Homo sapiens 112-121 33497181-9 2021 Concentrations of 2.5 x 1010 to 80 x 1010 CAT-SiNPs/mL not only proved to be nontoxic in cell cultures using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay but also conferred cell protection when cells were exposed to 1 mM hydrogen peroxide (H2O2). thiazolyl blue 113-173 catalase Rattus norvegicus 42-45 33415068-3 2020 In this study, we demonstrated that HHIP overexpression significantly suppressed the proliferation and invasion of AGS cells evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and transwell assays, respectively. thiazolyl blue 138-198 hedgehog interacting protein Homo sapiens 36-40 33517264-5 2021 MATERIALS AND METHODS: The cytotoxicity of human hepcidin against myeloma cells was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and DNA fragmentation assays. thiazolyl blue 96-157 hepcidin antimicrobial peptide Homo sapiens 49-57 33390818-6 2021 Subsequently, the impact of CENPF expression on cell proliferation, cell cycle, apoptosis, colony formation was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), flow cytometric analysis and colony formation assay, respectively. thiazolyl blue 128-189 centromere protein F Mus musculus 28-33 32935389-8 2021 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and apoptotic assays showed that SOX11 downregulation inhibited cell viability and induced apoptosis in cardiomyocytes. thiazolyl blue 0-60 SRY-box transcription factor 11 Homo sapiens 94-99 32360175-4 2021 METHODS: Viability of GroEL-treated osteoblasts was analyzed with 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide. thiazolyl blue 66-126 heat shock protein family D (Hsp60) member 1 Homo sapiens 22-27 33332155-7 2020 Further WB (Western Blot), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and flow cytometry confirmed that XIST could reverse the expression of AVEN and ameliorate HG-induced apoptosis. thiazolyl blue 32-92 X inactive specific transcript Homo sapiens 129-133 33828376-7 2020 SF1 was screened for cytotoxicity activity toward HeLa, SiHa, and normal cells (NIH) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. thiazolyl blue 94-155 splicing factor 1 Homo sapiens 0-3 33011625-8 2020 In vitro MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cytotoxic activity data showed that PdL1 was the most potent complex against MCF7 breast cancer cells; its IC50 value is lower than that of cisplatin. thiazolyl blue 14-74 CD274 molecule Homo sapiens 112-116 33245102-4 2020 Moreover, FAM225A knockdown significantly weakened HepG2/SOR cells resistance to sorafenib treatment by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. thiazolyl blue 109-169 family with sequence similarity 225 member A Homo sapiens 10-17 32818379-6 2020 It is also found that ulvan greatly reduces Abeta-induced cytotoxicity by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 78-138 amyloid beta precursor protein Rattus norvegicus 44-49 33023532-5 2020 Sensitivity of the cell lines in response to chemotherapy after TIMP-2 knock down was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and 5-Ethynyl-2"-deoxyuridine (EdU) assays. thiazolyl blue 102-162 TIMP metallopeptidase inhibitor 2 Homo sapiens 64-70 31989649-5 2020 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay showed selective cancer cell death depending on the CD44 expression in a time-dependent manner. thiazolyl blue 4-56 CD44 molecule (Indian blood group) Homo sapiens 121-125 31989649-5 2020 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay showed selective cancer cell death depending on the CD44 expression in a time-dependent manner. thiazolyl blue 58-61 CD44 molecule (Indian blood group) Homo sapiens 121-125 32909483-5 2020 RESULTS: We found that as the concentration of PTHrP increased, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT, a tetrazolium salt) colorimetric absorbance values (A values) decreased. thiazolyl blue 64-126 parathyroid hormone-like hormone Rattus norvegicus 47-52 32052444-4 2020 Effects of KIF18B expression on biological behaviors of HCC cells were detected by clone formation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and transwell assays. thiazolyl blue 100-160 kinesin family member 18B Homo sapiens 11-17 32668390-8 2020 By conducting 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), scratch test, and Transwell and flow cytometric analyses, miR-203 was witnessed to restrain SCL-1 cell proliferation, migration, and invasion while accelerating their apoptosis. thiazolyl blue 14-75 microRNA 203a Homo sapiens 141-148 32735017-4 2020 The effect of TRIM52 on LPS-induced inflammatory injury was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry, and enyzme-linked immunosorbent assay. thiazolyl blue 76-136 tripartite motif containing 52 Homo sapiens 14-20 32567557-1 2020 MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is widely used in biology as a probe for cell viability by virtue of its ability to generate deposits of insoluble formazan at sites of intense oxidoreductase activity. thiazolyl blue 5-65 thioredoxin reductase 1 Homo sapiens 211-225 32237054-6 2020 Data from MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) and flow cytometry assays showed that the decreased cell viability and induced cell apoptosis by LPS treatment were also reversed by miR-495 over-expression. thiazolyl blue 15-75 microRNA 495 Homo sapiens 210-217 32727779-6 2020 MATERIALS AND METHODS: PRDX2 inhibition of alcohol-induced apoptosis in L02 hepatocytes was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, fluorescence microscopy, flow cytometry, western blotting and hematoxylin and eosin staining. thiazolyl blue 104-164 peroxiredoxin 2 Mus musculus 23-28 31904145-6 2020 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that upon transfection with SMO shRNA, cell growth was decreased and drug sensitivity to cisplatin was upregulated. thiazolyl blue 0-60 smoothened, frizzled class receptor Homo sapiens 102-105 32760277-2 2020 We found that in vitro ICA or BMP-2 treatment is able to increase osteoblast proliferation, which was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). thiazolyl blue 114-174 bone morphogenetic protein 2 Homo sapiens 30-35 32211853-5 2020 The effect of miR-1323 on BC cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assay. thiazolyl blue 66-126 microRNA 1323 Homo sapiens 14-22 32575426-3 2020 Methods: The primary human Schwann cell (hSC) was characterized, and the proliferation rate of hSC supplemented with various concentrations of HT was determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 165-225 fucosyltransferase 1 (H blood group) Homo sapiens 95-98 31898360-10 2020 Further analysis by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay indicated a reduction in the survival and proliferation rates of SW480 cells overexpressing GATA4-miR1, but no impact was observed on apoptosis progression, as indicated by Annexin-V flow cytometry. thiazolyl blue 20-82 GATA binding protein 4 Homo sapiens 181-186 31898360-10 2020 Further analysis by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay indicated a reduction in the survival and proliferation rates of SW480 cells overexpressing GATA4-miR1, but no impact was observed on apoptosis progression, as indicated by Annexin-V flow cytometry. thiazolyl blue 20-82 fibronectin type III and SPRY domain containing 1 Homo sapiens 187-191 31943292-4 2020 The cytotoxicity of asparaginase in SCC-9 (tongue squamous cell carcinoma) and HaCaT (human keratinocyte) cell lines was evaluated with MTT cell viability assay. thiazolyl blue 136-139 asparaginase Homo sapiens 20-32 32056303-8 2020 RESULTS: MTT and flow cytometry analyses showed that miR-22 repressed MC3T3-E1 cell viability and promoted cell apoptosis. thiazolyl blue 9-12 microRNA 22 Mus musculus 53-59 32456005-9 2020 The result of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay suggested that the silver SPE on C-2 was non-cytotoxic toward L929 fibroblast cells proliferation. thiazolyl blue 19-80 complement component 2 (within H-2S) Mus musculus 121-124 31987787-5 2020 The effects of HASPIN knockdown on cell proliferation, clone-formation ability and apoptosis were determined by MTT, clone formation assay, flow cytometry and Human Apoptosis Antibody Array in vitro. thiazolyl blue 112-115 histone H3 associated protein kinase Homo sapiens 15-21 32425721-5 2020 Overexpression of miR-520f promoted cell proliferation by using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation, anchorage-independent growth assay, and 5-bromo-2-deoxyuridine assays. thiazolyl blue 68-129 microRNA 520f Homo sapiens 18-26 32032743-9 2020 Flow cytometry and MTT assay indicated cell death reduction and viability elevation effect of hsa-miR-766 in SW480 cells after its overexpression. thiazolyl blue 19-22 microRNA 766 Homo sapiens 94-105 31960754-7 2020 These results provide new pharmacological evidence that ALP facilitates neuroprotection via prevention of neuronal oxidative stress and promotion of cell survival signaling pathways.Abbreviations: ABTS: 2,2"-azino-bis-(3-ethylbenzothiazoline-6-sulfonicacid); AD: Alzheimer"s disease; ALP: polysaccharide extracts isolated from Annona muricata leaves; ARE: antioxidant response element; DPPH: 1,1-diphenyl-picrylhydrazyl; DCFH-DA: 2",7"-dichlorofluorescin diacetate; ECL: electrochemiluminescence; ERK: extracellular regulated kinase; FBS: Fetal bovine serum; FITC: fluorescein isothiocyanate; FRAP: ferric reducing antioxidant power; HO-1: Heme oxygenase-1; JNK: c-jun N-terminal kinase; MAPKs: mitogen-activated protein kinases; MDA: malondialdehyde; MMP: mitochondrial membrane potential; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide; NQO1: NAD(P)H:quinine oxidoreductase 1, Nrf2: nuclear factor-E2-related factor 2; PD: parkinson"s disease; PI3K: phosphatidylinositol-3kinase; PVDF: polyvinylidene difluoride; ROS: reactive oxygen species; SOD: Superoxidedismutase; TPTZ: tripydyltriazine. thiazolyl blue 791-794 alopecia, recessive Mus musculus 56-59 31960754-7 2020 These results provide new pharmacological evidence that ALP facilitates neuroprotection via prevention of neuronal oxidative stress and promotion of cell survival signaling pathways.Abbreviations: ABTS: 2,2"-azino-bis-(3-ethylbenzothiazoline-6-sulfonicacid); AD: Alzheimer"s disease; ALP: polysaccharide extracts isolated from Annona muricata leaves; ARE: antioxidant response element; DPPH: 1,1-diphenyl-picrylhydrazyl; DCFH-DA: 2",7"-dichlorofluorescin diacetate; ECL: electrochemiluminescence; ERK: extracellular regulated kinase; FBS: Fetal bovine serum; FITC: fluorescein isothiocyanate; FRAP: ferric reducing antioxidant power; HO-1: Heme oxygenase-1; JNK: c-jun N-terminal kinase; MAPKs: mitogen-activated protein kinases; MDA: malondialdehyde; MMP: mitochondrial membrane potential; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide; NQO1: NAD(P)H:quinine oxidoreductase 1, Nrf2: nuclear factor-E2-related factor 2; PD: parkinson"s disease; PI3K: phosphatidylinositol-3kinase; PVDF: polyvinylidene difluoride; ROS: reactive oxygen species; SOD: Superoxidedismutase; TPTZ: tripydyltriazine. thiazolyl blue 796-855 alopecia, recessive Mus musculus 56-59 32431515-5 2020 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was implemented to detect cell proliferation and the half maximal inhibitory concentration (IC50) of cisplatin (DDP) in vitro. thiazolyl blue 0-60 translocase of inner mitochondrial membrane 8A Homo sapiens 185-188 32373947-5 2020 The functions of miR-489 were analyzed by performing functional assays, such as MTT (3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays and transwell assays. thiazolyl blue 85-147 microRNA 489 Homo sapiens 17-24 31605575-7 2020 It was worth mentioning that in vitro [methylthio tetrazole (MTT) study in the MG63 cells] and in vivo (anticancer study in the MG63 xenograft model) assays both revealed that FA/MOF/DOX nanoparticles possessed stronger anticancer capability than free DOX or MOF/DOX nanoparticles. thiazolyl blue 61-64 lysine acetyltransferase 8 Homo sapiens 176-186 32149762-6 2020 The survival of HK-2 cells was determined using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) and Annexin V-Light 650/propidium iodide (PI) assays. thiazolyl blue 48-110 hexokinase 2 Homo sapiens 16-20 32007591-13 2020 MTT and flow cytometry results demonstrated that AS1411 aptamer could enhance both toxicity and cellular uptake in nucleolin overexpressing cell lines (P<0.05). thiazolyl blue 0-3 nucleolin Mus musculus 115-124 32056164-5 2020 The function of miR-498 was investigated through MTT and transwell assays. thiazolyl blue 49-52 microRNA 498 Homo sapiens 16-23 31566725-3 2020 Meanwhile, cell migration and cell proliferation with SDF-1 treated were examined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and transwell permeable assay, respectively. thiazolyl blue 87-147 C-X-C motif chemokine ligand 12 Homo sapiens 54-59 32197031-6 2020 The role of CTGF in the proliferation, migration, and invasion of gliomas were respectively identified by methylthiazoletetrazolium assay, Transwell and Boyden assay in vitro. thiazolyl blue 106-131 cellular communication network factor 2 Homo sapiens 12-16 32012442-0 2020 Amino Alcohol Acrylonitriles as Activators of the Aryl hydrocarbon Receptor Pathway, An Unexpected MTT Phenotypic Screening Outcome. thiazolyl blue 99-102 aryl hydrocarbon receptor Homo sapiens 50-75 31702994-8 2020 Annexin V analysis and MTT assay showed that miR-6165 overexpression was followed by increased apoptosis and reduced the viability rate of SW480 cells. thiazolyl blue 23-26 microRNA 6165 Homo sapiens 45-53 31650323-3 2020 METHODS: This study investigated COL1A1-014 for its biological function at both cellular and animal levels, using MTT, flow cytometry, colony formation and transwell assays. thiazolyl blue 114-117 collagen, type I, alpha 1 Mus musculus 33-39 31718978-8 2020 PB1-1-ConA and PB1-1-HSA were characterized with the lowest cytotoxicity during the MTT assay, and thus should be more suitable for live imaging applications than PB1-ConA and PB1-HSA. thiazolyl blue 84-87 submaxillary gland androgen regulated protein 3A Homo sapiens 0-5 31718978-8 2020 PB1-1-ConA and PB1-1-HSA were characterized with the lowest cytotoxicity during the MTT assay, and thus should be more suitable for live imaging applications than PB1-ConA and PB1-HSA. thiazolyl blue 84-87 submaxillary gland androgen regulated protein 3A Homo sapiens 15-20 31718978-8 2020 PB1-1-ConA and PB1-1-HSA were characterized with the lowest cytotoxicity during the MTT assay, and thus should be more suitable for live imaging applications than PB1-ConA and PB1-HSA. thiazolyl blue 84-87 submaxillary gland androgen regulated protein 3A Homo sapiens 0-3 31718978-8 2020 PB1-1-ConA and PB1-1-HSA were characterized with the lowest cytotoxicity during the MTT assay, and thus should be more suitable for live imaging applications than PB1-ConA and PB1-HSA. thiazolyl blue 84-87 submaxillary gland androgen regulated protein 3A Homo sapiens 15-18 31620995-6 2020 The result of an MTT assay confirmed the excellent biocompatibility of electroactive CNF/Gel scaffolds. thiazolyl blue 17-20 NPHS1 adhesion molecule, nephrin Homo sapiens 85-88 31742430-4 2020 The influence of SCUBE3 on the breast cancer cell proliferation, invasion, and migration was detected using methyl thiazolyl tetrazolium, wound healing, colony formation, and transwell assay. thiazolyl blue 108-136 signal peptide, CUB domain and EGF like domain containing 3 Homo sapiens 17-23 31860779-3 2020 Using human enterocytic-like Caco-2 cells, we have previously shown that Cd may induce a concentration and time-dependent increase in 3-(4,5-dimethyl-2-thiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MTT)-reducing activity in differentiated cultures with correlation to ERK1/2 activation. thiazolyl blue 134-197 mitogen-activated protein kinase 3 Homo sapiens 275-281 31917985-6 2020 Further, the cytotoxic effect of the synthesized Au NPs against MCF-7 cell lines was assessed by MTT assay with IC50 value of 250 mug mL-1. thiazolyl blue 97-100 L1 cell adhesion molecule Mus musculus 134-138 31922225-5 2020 In the present study, the effects of CDC25A on cell proliferation and migration were analyzed using cell cycle, MTT and Transwell assays. thiazolyl blue 112-115 cell division cycle 25A Homo sapiens 37-43 31704243-4 2020 This study elucidated that increased expression of TSGA10 in manipulated human umbilical vein endothelial cells (HUVECs) decreased the proliferation and migration of these cells as affirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and wound healing tests, respectively. thiazolyl blue 193-253 testis specific 10 Homo sapiens 51-57 32050643-5 2020 In addition, our in vitro gain-of-function study in three different HCC cell lines revealed that overexpression of MFG-E8 promoted the proliferation and migration of HCC cells, as determined by RT-qPCR, MTT assays, and wound healing analyses. thiazolyl blue 203-206 milk fat globule EGF and factor V/VIII domain containing Homo sapiens 115-121 32063193-8 2020 The MTT assays were used to find optimal concentrations of formaldehyde and AAT. thiazolyl blue 4-7 serpin family A member 1 Homo sapiens 76-79 32071459-11 2020 RESULTS: MTT assay showed that 1-100 mumol/L aspirin or meloxicam significantly promoted the proliferation of hDPC in a concentration dependent manner (P<0.05). thiazolyl blue 9-12 decapping mRNA 2 Homo sapiens 110-114 31669650-5 2020 An MTT assay of TU212 and Hep-2 cells showed increased cell proliferation after transfection with overexpressed CASC9 and decreased cell proliferation after transfection with silenced CASC9. thiazolyl blue 3-6 cancer susceptibility 9 Homo sapiens 112-117 31669650-5 2020 An MTT assay of TU212 and Hep-2 cells showed increased cell proliferation after transfection with overexpressed CASC9 and decreased cell proliferation after transfection with silenced CASC9. thiazolyl blue 3-6 cancer susceptibility 9 Homo sapiens 184-189 31809037-5 2020 MTT assays in low-density lipoprotein receptors (LDLRs) overexpressing SMMC-7721 human liver cancer cells showed ApoE density-dependent antitumor potency of ApoE-Ms-SF, in which 7.5% ApoE led to the best antitumor effect (IC50: 8.5 vs 23.3 mug/mL for free SF). thiazolyl blue 0-3 apolipoprotein E Homo sapiens 113-117 31809037-5 2020 MTT assays in low-density lipoprotein receptors (LDLRs) overexpressing SMMC-7721 human liver cancer cells showed ApoE density-dependent antitumor potency of ApoE-Ms-SF, in which 7.5% ApoE led to the best antitumor effect (IC50: 8.5 vs 23.3 mug/mL for free SF). thiazolyl blue 0-3 apolipoprotein E Homo sapiens 157-161 31809037-5 2020 MTT assays in low-density lipoprotein receptors (LDLRs) overexpressing SMMC-7721 human liver cancer cells showed ApoE density-dependent antitumor potency of ApoE-Ms-SF, in which 7.5% ApoE led to the best antitumor effect (IC50: 8.5 vs 23.3 mug/mL for free SF). thiazolyl blue 0-3 apolipoprotein E Homo sapiens 157-161 31802650-4 2020 METHODS: MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide) was used to detect cell viability, immunofluorescent staining was used to detect autophagy puncta, and luciferase reporter system was used to determine binding ability between miR-34a and NEAT1 or putative targets. thiazolyl blue 9-12 microRNA 34a Homo sapiens 256-263 32013999-4 2020 The function of miR-192 was investigated through MTT, Transwell, and dual-luciferase reporter assays. thiazolyl blue 49-52 microRNA 192 Homo sapiens 16-23 31802650-4 2020 METHODS: MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide) was used to detect cell viability, immunofluorescent staining was used to detect autophagy puncta, and luciferase reporter system was used to determine binding ability between miR-34a and NEAT1 or putative targets. thiazolyl blue 9-12 nuclear paraspeckle assembly transcript 1 Homo sapiens 268-273 31916393-17 2020 Furthermore, according to the results of the MTT assay, SOX11 promoted the proliferation of BMSC (1.064 +- 0.093 vs 0.747 +- 0.090, P < 0.001). thiazolyl blue 45-48 SRY-box transcription factor 11 Rattus norvegicus 56-61 31661371-4 2020 Moreover, the in vitro experiments showed that overexpression of miR-519d-3p suppressed cell proliferation and induced cell cycle G0/G1 phase arrest using MTT and flow cytometry assays in glioma cell lines, U87 and U251. thiazolyl blue 155-158 microRNA 519d Homo sapiens 65-73 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 224-227 cyclin D1 Homo sapiens 59-64 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 224-227 microRNA 519d Homo sapiens 68-76 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 224-227 microRNA 519d Homo sapiens 135-143 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 224-227 cyclin D1 Homo sapiens 166-171 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 224-227 cyclin D1 Homo sapiens 173-182 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 229-291 cyclin D1 Homo sapiens 59-64 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 229-291 microRNA 519d Homo sapiens 68-76 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 229-291 microRNA 519d Homo sapiens 135-143 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 229-291 cyclin D1 Homo sapiens 166-171 31661371-8 2020 Taken together, these data demonstrate that suppression of CCND1 by miR-519d-3p might be a therapeutic target for glioma.Abbreviations miR-519d-3p: microRNA-519d-3p; CCND1: Cyclin D1; ATCC: American Type Culture Collection; MTT: 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; PI: propidium iodide; WT: wild type; MUT: mutant type; SD: standard deviation. thiazolyl blue 229-291 cyclin D1 Homo sapiens 173-182 31881456-6 2020 Besides, the MTT assay suggested that the selected compounds could inhibit the proliferation of LSD1-overexpressed cancer cells. thiazolyl blue 13-16 lysine demethylase 1A Homo sapiens 96-100 32141531-11 2020 Results of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays and transwell assays indicated that miR-449a overexpression significantly repressed OS cell proliferation, invasion, and migration. thiazolyl blue 16-77 microRNA 449a Homo sapiens 122-130 31860165-4 2020 By knocking down and overexpressing DANCR, we assessed its significance in regulating viability (MTT assay), migration/invasion (Transwell assay), epithelial-mesenchymal transition (Western blot), stemness (mammosphere formation assay and Western blot), and production of inflammatory cytokines (qRT-PCR and ELISA) of breast cancer cells in vitro, as well as xenograft growth in vivo. thiazolyl blue 97-100 differentiation antagonizing non-protein coding RNA Homo sapiens 36-41 32027261-6 2020 MTT assay showed that the proliferation level of HuT 78 cells transfected with miRNA-145 mimic was significantly lower than that of blank control and miRNA-NC group (P<0.05). thiazolyl blue 0-3 microRNA 145 Homo sapiens 79-88 32038231-4 2019 Methods: We assessed the cytotoxicity of ATLIII and IFN-gamma on lung cancer cells by MTT. thiazolyl blue 86-89 interferon gamma Mus musculus 52-61 31575111-13 2020 Both freshPRP and FD-PRP significantly induced osteoblast proliferation in MTT cell viability assays. thiazolyl blue 75-78 proline rich protein 2-like 1 Rattus norvegicus 10-13 31714543-4 2020 In vitro MTT experiments strongly verified that the combination of temsirolimus with polymeric CA4-NPs exhibited an additive toxicity to 4T1 cells. thiazolyl blue 9-12 carbonic anhydrase 4 Mus musculus 95-98 31715371-4 2020 However, we got a completely opposite result that CA8 promoted RCC progression, those are CA8 overexpression promoted the proliferative and migratory ability of Caki-1 and 769-P cells in vitro as determined with MTT and transwell assay, and CA8 overexpression could also promote Caki-1 xenograft growth in BALB/C-nu/nu mice. thiazolyl blue 212-215 carbonic anhydrase 8 Homo sapiens 90-93 31715371-4 2020 However, we got a completely opposite result that CA8 promoted RCC progression, those are CA8 overexpression promoted the proliferative and migratory ability of Caki-1 and 769-P cells in vitro as determined with MTT and transwell assay, and CA8 overexpression could also promote Caki-1 xenograft growth in BALB/C-nu/nu mice. thiazolyl blue 212-215 carbonic anhydrase 8 Homo sapiens 90-93 32716350-5 2020 The function of miR-629 was investigated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and Transwell assays. thiazolyl blue 49-110 microRNA 629 Homo sapiens 16-23 31957818-11 2020 MiR-149 overexpression significantly suppressed the OC cell proliferation, invasion, and migration as determined by functional assays, including MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays and transwell assays. thiazolyl blue 145-148 microRNA 149 Homo sapiens 0-7 31957818-11 2020 MiR-149 overexpression significantly suppressed the OC cell proliferation, invasion, and migration as determined by functional assays, including MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays and transwell assays. thiazolyl blue 150-211 microRNA 149 Homo sapiens 0-7 32016976-4 2020 RESULTS: MTT assay showed that miR-200c could inhibit the proliferation of MDA-MB-231 and BT-549 cells. thiazolyl blue 9-12 microRNA 200c Homo sapiens 31-39 32072885-5 2020 Afterward, the retrieved rTC-1 was used in MTT assay against mouse colon adenocarcinoma, hepatocellular carcinoma, chondrosarcoma, mouse melanoma, and breast adenocarcinoma cell lines to investigate its probable anticancer application. thiazolyl blue 43-46 Serum cholesterol level QTL 22 Rattus norvegicus 25-30 32072885-9 2020 The IC50 results of MTT assay revealed that rTC-1 has cytotoxic effect on C26-A and SW1353 cancerous cell lines. thiazolyl blue 20-23 Serum cholesterol level QTL 22 Rattus norvegicus 44-49 31789346-8 2019 MTT, apoptosis and cell cycle assay results revealed that trophoblast cells transfected with miR-126a-3p mimic or si-ADAM9 exhibited higher proliferative activity and a lower apoptotic rate compared with the blank group (all P&lt;0.05). thiazolyl blue 0-3 membrane associated ring-CH-type finger 8 Rattus norvegicus 93-96 31789346-8 2019 MTT, apoptosis and cell cycle assay results revealed that trophoblast cells transfected with miR-126a-3p mimic or si-ADAM9 exhibited higher proliferative activity and a lower apoptotic rate compared with the blank group (all P&lt;0.05). thiazolyl blue 0-3 ADAM metallopeptidase domain 9 Rattus norvegicus 117-122 31647948-5 2019 Inhibitory rate and IC50 for DDP were detected using MTT method after DDP treatment. thiazolyl blue 53-56 translocase of inner mitochondrial membrane 8A Homo sapiens 29-32 31915594-7 2019 Results: The MTT assay showed that recombinant protein FAM172A isoform 1 (FAM172A-1) could induce HepG2 cell proliferation at the concentration of 10-100 ng/mL, while protein FAM172A isoform 3 (FAM172A-3) was at the concentration of 80-100 ng/mL. thiazolyl blue 13-16 family with sequence similarity 172 member A Homo sapiens 47-62 31915594-7 2019 Results: The MTT assay showed that recombinant protein FAM172A isoform 1 (FAM172A-1) could induce HepG2 cell proliferation at the concentration of 10-100 ng/mL, while protein FAM172A isoform 3 (FAM172A-3) was at the concentration of 80-100 ng/mL. thiazolyl blue 13-16 family with sequence similarity 172 member A Homo sapiens 55-62 31915594-7 2019 Results: The MTT assay showed that recombinant protein FAM172A isoform 1 (FAM172A-1) could induce HepG2 cell proliferation at the concentration of 10-100 ng/mL, while protein FAM172A isoform 3 (FAM172A-3) was at the concentration of 80-100 ng/mL. thiazolyl blue 13-16 family with sequence similarity 172 member A Homo sapiens 74-81 31570818-5 2019 The regulatory relationships between miR-21 and PTEN/PDCD4 were further validated by real-time PCR, western blot, MTT assay, and flow cytometry. thiazolyl blue 114-117 microRNA 21 Rattus norvegicus 37-43 31570818-5 2019 The regulatory relationships between miR-21 and PTEN/PDCD4 were further validated by real-time PCR, western blot, MTT assay, and flow cytometry. thiazolyl blue 114-117 phosphatase and tensin homolog Rattus norvegicus 48-52 31111481-5 2019 Moreover, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay results revealed that increased miR-200c expression and cisplatin can more inhibited the proliferation of KATOIII cells. thiazolyl blue 10-70 microRNA 200c Homo sapiens 109-117 31791536-5 2019 The Nok-si cell viability was evaluated by using a 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay (MTT), morphology by scanning electron microscopy (SEM), and proliferation and adhesion by using a confocal microscope. thiazolyl blue 51-112 serine/threonine/tyrosine kinase 1 Homo sapiens 4-7 31791536-5 2019 The Nok-si cell viability was evaluated by using a 3-(4.5-dimethylthiazole-2-yl)2.5-diphenyl tetrazolium bromide assay (MTT), morphology by scanning electron microscopy (SEM), and proliferation and adhesion by using a confocal microscope. thiazolyl blue 120-123 serine/threonine/tyrosine kinase 1 Homo sapiens 4-7 31702814-10 2019 An MTT assay revealed that miR-217 inhibitor significantly increased the viability and decreased the apoptosis of high glucose-induced ARPE-19 cells. thiazolyl blue 3-6 microRNA 217 Homo sapiens 27-34 31839043-2 2019 METHODS: The cDNA of TFPI-2 was obtained by genetic chemical synthesis, the TFPI-2 gene and the linear vector fragment were ligated and inserted into the multiple cloning site of PEGFP-N1 vector, and the eukaryotic expression vector PEGFP-N1-TFPI-2 was transfected SHI-1 cells, then the obtained SHI-1 cells was observed by fluorescence microscopy; MTT assay was used to detect the effect of TFPI-2 gene on the relative growth rate of SHI-1 cells at the different time-point; RT-PCR was used to detect TFPI-2 mRNA expression levels in the cells of each group before and after TFPI-2 transfection; TFPI-2 protein expression was detected by Western blot. thiazolyl blue 349-352 tissue factor pathway inhibitor 2 Homo sapiens 21-27 31839059-6 2019 RESULTS: MTT assay results showed that the proliferation capacity of cells in DIS3 over-expression group was significantly reduced, as compared with the DIS3 over-expression-empty vector group at the same time point (24, 48 or 72 h) (P<0.05, P<0.01). thiazolyl blue 9-12 DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease Homo sapiens 78-82 32009796-5 2019 The function of TP73-AS1 in lung adenocarcinoma progression was estimated by MTT assay, EdU assay, flow cytometry, Western blot, wound-healing assay and transwell assay. thiazolyl blue 77-80 tumor protein p73 Homo sapiens 16-20 31545954-9 2019 Furthermore, tomatidine below 8 muM showed more than 80% of cell viability in MTT assay. thiazolyl blue 78-81 latexin Homo sapiens 32-35 31933795-3 2019 The proliferation of thyroid cancer stem cells after IL-6 treatment was assessed by the MTT method. thiazolyl blue 88-91 interleukin 6 Homo sapiens 53-57 31661774-5 2019 MTT assay was used to detect the effect of eleutheroside B on proliferation of human HK-2 cells in presence and in absence of cisplatin. thiazolyl blue 0-3 hexokinase 2 Homo sapiens 85-89 31655798-5 2019 Our in vitro colony-formation and MTT assays showed that cell viability, number, and the size of clonogenicities were all lower in the presence of T5224, RSPO2, or AZD536 than in controls. thiazolyl blue 34-37 R-spondin 2 Homo sapiens 154-159 32010307-5 2020 When VEGFR2 was inhibited by axitinib, VEGFR2 protein expression was determined by western blotting, and the cell viability was assessed by MTT analysis. thiazolyl blue 140-143 kinase insert domain receptor Homo sapiens 5-11 31894331-7 2020 Light microscopy, MTT and flow cytometric assays revealed that SIRT3 overexpression inhibited growth and promoted apoptosis in NCI-H446 human small cell lung cancer cells. thiazolyl blue 18-21 sirtuin 3 Homo sapiens 63-68 31883226-5 2020 The effect of miR-4317 on proliferation was evaluated through 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2Htetrazolium bromide (MTT) assay. thiazolyl blue 127-130 microRNA 4317 Homo sapiens 14-22 32071790-6 2020 aSyn cytotoxicity was assessed by the MTT and vital-dye exclusion methods, observed at the beginning of the sub-culture of low-aSyn overexpressing neurons when cells can barely proliferate exponentially. thiazolyl blue 38-41 synuclein alpha Homo sapiens 0-4 31940916-5 2020 An MTT assay showed that ABCG2 overexpression significantly desensitized both the cancer cells and ABCG2 transfected-HEK293 cells to tivantinib and that this drug resistance can be reversed by ABCG2 inhibitors. thiazolyl blue 3-6 ATP binding cassette subfamily G member 2 (Junior blood group) Homo sapiens 25-30 31907045-6 2020 The in vitro MTT assay indicated that the cytotoxicity of DSF/SPC-NSps was enhanced remarkably compared to free DSF against the 4T1 cell line. thiazolyl blue 13-16 sparse coat Mus musculus 62-65 31654889-2 2020 Methyl thiazolyl tetrazolium (MTT) assays demonstrated that G1 and G3 exhibited excellent biocompatibility against HEK293 epithelial cells while G2 had an obvious cytotoxicity. thiazolyl blue 0-28 proline rich protein BstNI subfamily 3 Homo sapiens 60-69 31654889-2 2020 Methyl thiazolyl tetrazolium (MTT) assays demonstrated that G1 and G3 exhibited excellent biocompatibility against HEK293 epithelial cells while G2 had an obvious cytotoxicity. thiazolyl blue 30-33 proline rich protein BstNI subfamily 3 Homo sapiens 60-69 31957835-7 2020 After interfering in the expression of LINC01093, the results of MTT and colony forming assays revealed that the proliferation ability of NSCLC cells was weakened, according to the findings in the flow cytometry, the cells were arrested in G1/0 phase, the transwell assay results manifested that the cell migration and invasion abilities were weakened, and the results of the Western blotting suggested the changes in the expressions of molecular markers in the Akt signaling pathway. thiazolyl blue 65-68 long intergenic non-protein coding RNA 1093 Homo sapiens 39-48 31956370-5 2020 Moreover, the function of TRIM67 in cultured NSCLC cells was evaluated by MTT, colony formation, and Transwell assays. thiazolyl blue 74-77 tripartite motif containing 67 Homo sapiens 26-32 31750632-6 2020 Meanwhile, the modulatory influence of miR-522 on cell apoptosis and viability of Hela cells was also detected by flow cytometry and MTT assay. thiazolyl blue 133-136 microRNA 522 Homo sapiens 39-46 31897183-7 2020 The Celigo Cell Counting method, MTT and colony formation assay revealed that the proliferation rates of lung cancer cells were significantly suppressed when POLR1B was silenced by lentivirus-mediated RNA interference. thiazolyl blue 33-36 RNA polymerase I subunit B Homo sapiens 158-164 31897197-7 2020 MTT and colony formation assay results demonstrated that MALAT1 promoted GC cell proliferation. thiazolyl blue 0-3 metastasis associated lung adenocarcinoma transcript 1 Homo sapiens 57-63 31897213-5 2020 The regulatory mechanism of miR-431 was investigated using MTT, Transwell and luciferase reporter assay. thiazolyl blue 59-62 microRNA 431 Homo sapiens 28-35 32002394-7 2019 Biological activity of GM-CSF on HL-60 cells was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 65-125 colony stimulating factor 2 Homo sapiens 23-29 32002394-7 2019 Biological activity of GM-CSF on HL-60 cells was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 127-130 colony stimulating factor 2 Homo sapiens 23-29 32002394-10 2019 The biological activity of purified GM-CSF on HL-60 cell line was assessed by MTT assay, and the specific activity of produced GM-CSF was determined as 1.2 x 104 IU/mug. thiazolyl blue 78-81 colony stimulating factor 2 Homo sapiens 36-42 31858549-5 2019 Then, the function of SNHG14 was detected through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, colony formation assay, and transwell assay in vitro. thiazolyl blue 50-53 small nucleolar RNA host gene 14 Homo sapiens 22-28 31858549-5 2019 Then, the function of SNHG14 was detected through MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay, colony formation assay, and transwell assay in vitro. thiazolyl blue 55-116 small nucleolar RNA host gene 14 Homo sapiens 22-28 31788043-10 2019 MTT and Annexin V-fluorescein isothiocyanate/propidium iodide assays demonstrated that CLDN1 silencing significantly inhibits proliferation and enhances apoptosis induced by 5-FU treatment in Hep/5FU cells, compared with non-silenced Hep/5FU cells. thiazolyl blue 0-3 claudin 1 Homo sapiens 87-92 31771152-4 2019 MTT assay showed significant inhibition of MCF-7 cells viability with the IC50 value of 13.04 +- 1.03 microg/mL after 48 h. The compound induced down-regulation of anti-apoptotic Bcl-2 protein and increase in the pro-apoptotic Bax/Bcl-2 ratio in MCF-7 cells. thiazolyl blue 0-3 BCL2 apoptosis regulator Homo sapiens 179-184 31771152-4 2019 MTT assay showed significant inhibition of MCF-7 cells viability with the IC50 value of 13.04 +- 1.03 microg/mL after 48 h. The compound induced down-regulation of anti-apoptotic Bcl-2 protein and increase in the pro-apoptotic Bax/Bcl-2 ratio in MCF-7 cells. thiazolyl blue 0-3 BCL2 associated X, apoptosis regulator Homo sapiens 227-230 31771152-4 2019 MTT assay showed significant inhibition of MCF-7 cells viability with the IC50 value of 13.04 +- 1.03 microg/mL after 48 h. The compound induced down-regulation of anti-apoptotic Bcl-2 protein and increase in the pro-apoptotic Bax/Bcl-2 ratio in MCF-7 cells. thiazolyl blue 0-3 BCL2 apoptosis regulator Homo sapiens 231-236 31814886-13 2019 MTT indicated that the overexpression of miR-136-5p dramatically inhibited the proliferation of B-CPAP cells. thiazolyl blue 0-3 microRNA 136 Homo sapiens 41-48 31807022-3 2019 The effect of GOF-mutant SHP2 on proliferation was measured using the MTT assay, flow cytometry, colony formation assay, and soft agar assay. thiazolyl blue 70-73 protein tyrosine phosphatase non-receptor type 11 Homo sapiens 25-29 31685029-3 2019 METHODS: The underlying mechanisms and anticancer effect of C-2 in bladder cancer cells were investigated by MTT, western blotting, immunoprecipitation and immunofluorescence assays. thiazolyl blue 109-112 complement component 2 (within H-2S) Mus musculus 60-63 31499260-6 2019 Their in vitro cytotoxicities were screened against human oral squamous cell carcinoma (OSCC) cell lines (HSC-2) and human mesenchymal normal oral cells (HGF) via 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) test. thiazolyl blue 163-223 hepatocyte growth factor Homo sapiens 154-157 31602243-6 2019 The effects of miR-877 overexpression on cervical cancer cell proliferation and invasion were evaluated using MTT and Transwell cell invasion assays. thiazolyl blue 110-113 microRNA 877 Homo sapiens 15-22 31983096-10 2019 MTT assay showed that miR-532-3D remarkably suppressed the proliferation of NSCLC cells. thiazolyl blue 0-3 microRNA 532 Homo sapiens 22-29 31611991-4 2019 The knockdown of NSE markedly decreased the proliferation of NCI-H209 cells, as indicated by MTT assay (P<0.05). thiazolyl blue 93-96 enolase 2 Homo sapiens 17-20 31699188-1 2019 Objective To explore the role of multidrug resistance gene-1(MDR1)gene in methotrexate(MTX)resistance in patients with rheumatoid arthritis(RA).Methods Fibroblast-like synoviocytes(FLS)from RA patients were infected with recombinant adenovirus Ad-EGFP-MDR1 in vitro to obtain MDR1 over-expressed RA FLS.The transcription level of MDR1 gene and the expression level of its coding product P-glycoprotein(P-gp) rotein were detected by real-time PCR and Western blot analysis.The efflux function was verified by rhodamine 123 efflux assay.The resistance to MTX was detected by MTT assay.Results RA FLS were infected with recombinant adenovirus Ad-EGFP-MDR1;72 hours later,the particles size in MDR1 over-expressed RA FLS increased,the cell volume became larger,and the growth rate decreased.The transcription level of MDR1(1.4325+-0.3924 vs.0.0650+-0.0070;t=6.035,P=0.004),the expression level of P-gp protein(1.8667+-0.2857 vs. 0.9367+-0.0551;t=5.536,P=0.005),and the ability of extracellular rhodamine 123(979.43+-196.81 vs.1680.06+-147.04;t=-4.940,P=0.008) in MDR1 over-expressed RA FLS were significantly higher than those of negative virus control RA-FLS,and the survival rate of MDR1 over-expressed RA FLS was significantly increased at each concentration of MTX(P<0.05).Conclusion The high expression of MDR1 can affect the efflux ability to MTX by up-regulating the expression of P-gp,thus enhancing the drug resistance to MTX in RA FLS. thiazolyl blue 573-576 ATP binding cassette subfamily B member 1 Homo sapiens 61-65 31749617-4 2019 The cytotoxicity of alpha-syn/SWCNTs co-incubated with vitamin K1 against SH-SY5Y cells was also carried out by MTT, LDH, and caspase-3 assays. thiazolyl blue 112-115 synuclein alpha Homo sapiens 20-29 31802893-7 2019 The effect of ROS1 knockdown on cell proliferation, cell cycle distribution, cell apoptosis and metastasis in vitro was evaluated by MTT, colony formation, flow cytometric analysis, wound healing and Transwell invasion assays. thiazolyl blue 133-136 ROS proto-oncogene 1, receptor tyrosine kinase Homo sapiens 14-18 31749697-7 2019 We found that for the P284T variant of POR, the cytochrome c reduction activity was reduced to 47% of the WT and MTT reduction was reduced to only 15% of the WT. thiazolyl blue 113-116 cytochrome p450 oxidoreductase Homo sapiens 39-42 31966076-2 2020 Fifty pairs of NSCLC and para-cancerous tissues were collected to identify the expression level of miR-15a in NSCLC, para-cancerous tissue, and cell lines A549, H1299, H1975 and BEAS-2B by real-time fluorescence quantitative PCR (RT-PCR); A549 cells were transfected with miR-15a mimic; the MTT assay was performed to detect the role of miR-15a transfection in proliferation of A549 cells, the wound healing assay was carried out to identify the role of miR-15a in migration of A549 cells; Transwell invasion assay was conducted to analyze the role of miR-15a in invasion of A549 cells; western blotting was carried out to find the effect of miR-15a on Smad3 expression, and Spearman"s rank correlation was used to analyze the correlation between miR-15a and Smad3 expression. thiazolyl blue 291-294 microRNA 15a Homo sapiens 99-106 31572520-6 2019 Androgen-independent human prostate cancer cell lines PC3 and DU145 were transfected with pim-1-targeted or control shRNA, and MTT results revealed that pim-1 knockdown significantly inhibited PC3 and DU145 cell viability in a time-dependent manner (P<0.01). thiazolyl blue 127-130 Pim-1 proto-oncogene, serine/threonine kinase Homo sapiens 153-158 31572529-5 2019 Subsequently, Reg3A protein was added to the culture media at a final concentration of 50 ng/ml in the presence or absence of eckol for 24 h. The cytotoxicity and proliferative capacity of the SW1990 cells was determined using an MTT and flow cytometry analysis. thiazolyl blue 230-233 regenerating family member 3 alpha Homo sapiens 14-19 31243804-4 2019 In the present study, miR-579 was overexpressed in U251 and A-172 cell lines by using lentil vector, and its effect on cellular behavior such as proliferation and migration was investigated by the cell cycle, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Annexin V, colony formation, Transwell and wound healing assays. thiazolyl blue 209-269 microRNA 579 Homo sapiens 22-29 31696481-10 2019 MTT assay and transwell assay indicated that miR-26a up-regulation significantly repressed PTC cell viability, invasion, and metastasis. thiazolyl blue 0-3 microRNA 26a-1 Homo sapiens 45-52 31998458-8 2019 Results: MTT assay showed that ANGPTL8 inhibits proliferation of HepG2 cells moderately in a time-independent manner. thiazolyl blue 9-12 angiopoietin like 8 Homo sapiens 31-38 30955440-5 2019 The anti-osteosarcoma activity of CTLs evoked by DCs loaded with Hsp70-PCs was determined using Thiazolyl Blue Tetrazolium Bromide (MTT) assay. thiazolyl blue 96-130 heat shock protein family A (Hsp70) member 4 Homo sapiens 65-70 31933762-5 2019 The effect of miR-145 on cervical cancer proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 73-133 microRNA 145 Homo sapiens 14-21 31933762-5 2019 The effect of miR-145 on cervical cancer proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 135-138 microRNA 145 Homo sapiens 14-21 31357249-8 2019 Additionally, the effect of MAGL on tumor growth in EC was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide , cell cycle and western blotting assay in vitro. thiazolyl blue 71-131 monoglyceride lipase Homo sapiens 28-32 31541003-4 2019 The influences of miR-367 expression on cell proliferation, migration and invasion were analyzed using MTT and transwell assays respectively. thiazolyl blue 104-107 microRNA 367 Homo sapiens 18-25 31572671-6 2019 MTT proliferation, colony formation, migration and invasion assays and immunoblotting assay in NCI-H358 and A549 cell lines suggested that OTUD7B enhances EGF-induced Akt signal transduction and promotes lung cancer cell proliferation and migration. thiazolyl blue 0-3 OTU deubiquitinase 7B Homo sapiens 139-145 31662769-3 2019 The MTT assay was used to evaluate the proliferative activity of SMMC-7721 cells treated with EIB. thiazolyl blue 4-7 serine (or cysteine) peptidase inhibitor, clade B, member 1b Mus musculus 94-97 31934100-4 2019 PATIENTS AND METHODS: miR-130a mimics was used to up-regulate miR-130a expression in Doxorubicin-resistant MCF-7/Adr breast cancer cell line, followed by MTT assay and colony formation to determine cell viability and relative colony number. thiazolyl blue 154-157 microRNA 130a Homo sapiens 22-30 31336010-5 2019 In vitro, cell growth curves and MTT (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) assays showed that SALL4 promoted the cell proliferation of cervical cancer cells. thiazolyl blue 38-99 spalt like transcription factor 4 Mus musculus 120-125 31502580-5 2019 Functional experiments showed that miR-425-5p mimic inhibited DDP resistance as evidenced by a promoted apoptosis rate (flow cytometry) and suppressed cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay) and expressions of MDR1 andMRP1 (western blotting) in DU145/DDP and PC3/DDP cells. thiazolyl blue 167-227 microRNA 425 Homo sapiens 35-42 31695369-3 2019 Also, the cytotoxicity of alpha-syn amyloid either alone or with CeO2 NPs against neuron-like cells (SH-SY5Y) was examined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry, and quantitative real-time polymerase chain reaction (Bax and Bcl-2 gene expression) assays. thiazolyl blue 129-189 synuclein alpha Homo sapiens 26-35 30989725-5 2019 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to estimate the effect of miR-34a on the apoptosis of osteoblast. thiazolyl blue 0-60 microRNA 34a Homo sapiens 102-109 31273338-6 2019 The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) assay and flow cytometry showed that the decreased TAZ increased NPC cell paclitaxel chemosensitivity. thiazolyl blue 9-69 tafazzin, phospholipid-lysophospholipid transacylase Homo sapiens 122-125 31505885-5 2019 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cell viability assay found that miR-21 overexpression induced drug resistance to topoisomerase inhibitors (SN-38, doxorubicin, and etoposide/VP-16). thiazolyl blue 4-65 microRNA 21 Homo sapiens 104-110 31552220-6 2019 Here, we identified five molecules active on recurrent Y537S ERalpha polymorphism by employing in silico virtual screening on commercial databases of molecules, complemented by ER-transactivation and MTT assays in MCF7 and MDA-MB-231 breast cancer cells expressing wild type or mutated ERalpha. thiazolyl blue 200-203 estrogen receptor 1 Homo sapiens 61-68 31388510-10 2019 KLF5 overexpression in HCT116 stable cell line showed significantly better cell viability by increasing cyclinD1 and b-catenin compared to control cells in MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, suggesting that KLF5 mediates cell survival. thiazolyl blue 161-221 Kruppel like factor 5 Homo sapiens 0-4 31388510-10 2019 KLF5 overexpression in HCT116 stable cell line showed significantly better cell viability by increasing cyclinD1 and b-catenin compared to control cells in MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, suggesting that KLF5 mediates cell survival. thiazolyl blue 161-221 Kruppel like factor 5 Homo sapiens 246-250 30912183-4 2019 Furthermore, the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay showed that miR-448 inhibited cell viability in NSCLC. thiazolyl blue 22-82 microRNA 448 Homo sapiens 102-109 30975469-7 2019 Significantly, NETO2 knockdown promoted the radiotherapy in vitro, as evidenced by the further reduced cell proliferation and metastasis in NPC cells using 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide (MTT), colony formation and transwell analysis. thiazolyl blue 156-219 neuropilin and tolloid like 2 Homo sapiens 15-20 31646811-5 2019 The biological role of miR-489 on cell proliferation, cell cycle and apoptosis were determined in PANC-1 cells by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and flow cytometry after transfection with miR-NC, miR-489 mimics and si-AKT3. thiazolyl blue 114-117 microRNA 489 Homo sapiens 23-30 31646811-5 2019 The biological role of miR-489 on cell proliferation, cell cycle and apoptosis were determined in PANC-1 cells by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay and flow cytometry after transfection with miR-NC, miR-489 mimics and si-AKT3. thiazolyl blue 114-117 membrane associated ring-CH-type finger 8 Homo sapiens 23-26 30175946-4 2019 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on Neuro-2a neuroblastoma cell line showed comparably low cytotoxicity of Bcf-PLGA-NPs as compared with aqueous solution of Bcf at reported Cmax values of the drug. thiazolyl blue 5-65 sperm beat cross frequency Mus musculus 147-150 30849536-6 2019 Furthermore, results from Wound healing assay, Transwell assays, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)assay and 5-ethynyl-2"-deoxyuridine (EdU) assay revealed that miR-99b-3p played a promoting role in migration, invasion and proliferation of HCC cells. thiazolyl blue 65-125 microRNA 99b Homo sapiens 193-200 31555025-9 2019 The results of MTT test showed a significant decrease in PC3 and DU145 cell survival rate at 50, 100, and 500 mug/mL concentrations (P < 0.05). thiazolyl blue 15-18 chromobox 8 Homo sapiens 57-60 31298385-4 2019 The functions of miR-22 in melanoma cell proliferation, migration and invasion were investigated with functional assays, including MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and transwell assay. thiazolyl blue 136-197 microRNA 22 Homo sapiens 17-23 31115014-3 2019 The effects of KLF5 inhibition on cell proliferation and apoptosis were assessed by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and flow cytometer, respectively. thiazolyl blue 84-146 Kruppel like factor 5 Homo sapiens 15-19 32039714-11 2019 Besides, MTT and wound-healing assays revealed that LINC01420 inhibited cell migration in human melanoma cell line A375, but does not influence on cell viability. thiazolyl blue 9-12 negative regulator of P-body association Homo sapiens 52-61 31114994-5 2019 Then, the biological functions of DUXAP10 in prostate cancer cells, proliferation, migration, and apoptosis were studied by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, colony-formation assay, cell cycle analysis, transwell migration assay, wound healing assay, and cell apoptosis assay, respectively. thiazolyl blue 128-188 double homeobox A pseudogene 10 Homo sapiens 34-41 30317561-10 2019 5-Ethynyl-20-deoxyuridine and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed that overexpression of miR-208b promoted the proliferation of cattle primary myoblasts. thiazolyl blue 30-90 microRNA 208b Bos taurus 128-136 30317663-7 2019 To diagnose cytotoxic effect of HMGA2 siRNA and other components of transfection process, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was applied. thiazolyl blue 90-150 high mobility group AT-hook 2 Homo sapiens 32-37 30784209-5 2019 We further showed that compared with the empty vector, MALAT1 knockdown had significantly reduced the capacity for angiogenesis, which was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT), scratching, cell cycle and immunofluorescent staining. thiazolyl blue 151-203 metastasis associated lung adenocarcinoma transcript 1 Homo sapiens 55-61 31010200-4 2019 Furthermore, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the cytoprotective effects of 3-BDB were mediated by the activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB, Akt) signaling. thiazolyl blue 17-77 mitogen-activated protein kinase 1 Homo sapiens 167-204 31010200-4 2019 Furthermore, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that the cytoprotective effects of 3-BDB were mediated by the activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB, Akt) signaling. thiazolyl blue 17-77 mitogen-activated protein kinase 1 Homo sapiens 206-209 30556210-5 2019 Real-time polymerase chain reaction, Western blot analysis, computational analysis, and luciferase assay were conducted to establish a myocardial infarction associated transcript (MIAT) signaling pathway, whose role in the pathogenesis of AHL was further validated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and flow cytometry. thiazolyl blue 268-329 myocardial infarction associated transcript (non-protein coding) Mus musculus 180-184 30556210-5 2019 Real-time polymerase chain reaction, Western blot analysis, computational analysis, and luciferase assay were conducted to establish a myocardial infarction associated transcript (MIAT) signaling pathway, whose role in the pathogenesis of AHL was further validated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and flow cytometry. thiazolyl blue 268-329 cadherin 23 (otocadherin) Mus musculus 239-242 30915769-6 2019 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell activity assay kit was applied to determine the effects of miR-21 on the proliferation of VSMCs through regulating the Akt/ERK signaling pathway after the ERK signaling pathway inhibitor PD98059 and AKT inhibitor MK-2206 were given. thiazolyl blue 4-64 microRNA 21 Rattus norvegicus 135-141 30719181-5 2019 GRP94-knockdown (GRP94-KD) OS cells were generated using short hairpin RNAs, and the response to chemotherapy was assessed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. thiazolyl blue 137-197 heat shock protein 90 beta family member 1 Homo sapiens 0-5 30514177-5 2019 The effects of BAG3 knockdown on cell proliferation, cell invasion, and apoptosis were analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the in vitro 2-chamber invasion assay, and flow cytometry in BAG3 short hairpin RNA (shRNA)-transfected cells and control cells. thiazolyl blue 103-163 BAG cochaperone 3 Homo sapiens 15-19 31957415-3 2019 The cytocompatibility of the collagen-fibronectin gel was determined using MTT test and staining with PKH-26. thiazolyl blue 75-78 fibronectin 1 Rattus norvegicus 38-49 30366615-5 2019 Following transfection of miR-221 into cultured bovine mammary gland epithelial cells, inhibition of cell proliferation and reduced viability of these cells were observed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry analysis. thiazolyl blue 181-241 microRNA 221 Bos taurus 26-33 30216108-3 2019 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and Transwell assays showed that SCC-S2 significantly promoted the proliferation and invasion. thiazolyl blue 5-65 TNF alpha induced protein 8 Homo sapiens 100-106 30468461-7 2018 The effect of FOXA1 on apoptosis of HeLa cells was detected by using thiazolyl blue tetrazolium bromide (MTT) method. thiazolyl blue 69-103 forkhead box A1 Homo sapiens 14-19 30575916-4 2018 The effects of CXCL13 inhibition on cell proliferation and apoptosis were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and flow cytometry, respectively. thiazolyl blue 86-147 C-X-C motif chemokine ligand 13 Homo sapiens 15-21 30323244-3 2018 MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-di phenyltetrazolium bromide] and cell colony formation assays revealed that irisin significantly inhibited the growth of MIA PaCa-2 and Panc03.27 in a dose-dependent manner. thiazolyl blue 5-68 fibronectin type III domain containing 5 Homo sapiens 117-123 30610810-5 2018 The 3-(4, 5-dimethylthiazol-2-yl)-2), 5-diphenyltetrazolium bromide (MTT) assay was used to detect the antitumor effect of NaF on THP-1 cells in vitro. thiazolyl blue 4-67 C-X-C motif chemokine ligand 8 Homo sapiens 123-126 30280783-3 2018 The proliferation of lncRNA LET was detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide). thiazolyl blue 53-114 NPTN intronic transcript 1 Homo sapiens 21-31 31949552-9 2018 Significantly, Hoechst/PI double staining and an MTT assay proved integrin beta1 could contribute to cellular survival and 5FU resistance under translational control of eIF4E in these cells. thiazolyl blue 49-52 integrin subunit beta 1 Homo sapiens 66-80 30135397-5 2018 The entry of propidium iodide caused by CPE was well correlated with the loss of cell viability monitored by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. thiazolyl blue 113-173 cpe Clostridium perfringens 40-43 31938389-9 2018 Function of PANDAR on tumor cell proliferation, migration, and invasion was further evaluated by MTT and Transwell assays in vitro. thiazolyl blue 97-100 promoter of CDKN1A antisense DNA damage activated RNA Homo sapiens 12-18 30004169-3 2018 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cell proliferation and transwell assays revealed that miR-874-3p and miR-874-5p significantly inhibit EOC cell proliferation, migration, and invasion. thiazolyl blue 0-61 microRNA 874 Homo sapiens 122-129 30004169-3 2018 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cell proliferation and transwell assays revealed that miR-874-3p and miR-874-5p significantly inhibit EOC cell proliferation, migration, and invasion. thiazolyl blue 0-61 microRNA 874 Homo sapiens 137-144 29729269-3 2018 Here, we showed that LL-37 reduced human osteoblast-like MG63 cell viability assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and increased plasma membrane permeability determined by measuring intracellular Ca2+ levels and lactate dehydrogenase (LDH) release. thiazolyl blue 93-153 cathelicidin antimicrobial peptide Homo sapiens 21-26 30041403-8 2018 Finally, 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide (MTT) assays showed that Neuroglobin is an important neuroprotectant that protects SK-N-SH cells from TNF-alpha-induced decrease in cell viability. thiazolyl blue 9-69 neuroglobin Homo sapiens 95-106 29991107-8 2018 The effect of NGF on glioblastoma cell line (U87-MG) was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 70-132 nerve growth factor Homo sapiens 14-17 29793337-9 2018 The results of both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and antitumor efficiency elucidated that the DOX/TSMCN under NIR irradiation had a synergistic therapeutic effect for cancer. thiazolyl blue 20-80 NOC2 like nucleolar associated transcriptional repressor Homo sapiens 142-145 30168485-4 2018 Methods: The effect of human insulin and S961 on growth, proliferation rate and clonogenic potential of breast cancer cells was evaluated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] assay and clonogenic assay. thiazolyl blue 146-205 insulin Homo sapiens 29-36 29670371-6 2018 The effects of FSIP1 knockdown on tumor cells were assessed using colony formation, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and flow cytometry (FCM) apoptosis assays in vitro and BALB/c nude mouse xenograft model in vivo. thiazolyl blue 84-144 fibrous sheath-interacting protein 1 Mus musculus 15-20 29488611-7 2018 Finally, it was revealed that miR-205-5p restricted the proliferation ability of CRC cells through inhibiting PTK7, which was determined using colony forming and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. thiazolyl blue 162-222 microRNA 205 Homo sapiens 30-37 29488611-7 2018 Finally, it was revealed that miR-205-5p restricted the proliferation ability of CRC cells through inhibiting PTK7, which was determined using colony forming and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. thiazolyl blue 162-222 protein tyrosine kinase 7 (inactive) Homo sapiens 110-114 31938202-5 2018 RESULTS: The optical density value of A375/H19+ cells increased after incubation with MTT reagent for 12 h (P < 0.05), and the transwell assay showed that the average penetration rate of A375/H19+ cells significantly increased (P < 0.001). thiazolyl blue 86-89 H19 imprinted maternally expressed transcript Homo sapiens 43-46 29630117-5 2018 The effect of URG4 on CNE4 and HONE1 cells viability was determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). thiazolyl blue 72-133 upregulator of cell proliferation Homo sapiens 14-18 29573252-7 2018 The inflammatory responses and relevant cellular responses in IEC-6 cells influenced by IGFBP-3 expression were evaluated by western blotting, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and reactive oxygen species (ROS) measurement. thiazolyl blue 143-203 insulin-like growth factor binding protein 3 Rattus norvegicus 88-95 29513007-4 2018 The cytotoxicity of BPP toward OS cell lines U2OS and MG-63 was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 74-134 sushi repeat containing protein X-linked 2 Homo sapiens 20-23 31938202-5 2018 RESULTS: The optical density value of A375/H19+ cells increased after incubation with MTT reagent for 12 h (P < 0.05), and the transwell assay showed that the average penetration rate of A375/H19+ cells significantly increased (P < 0.001). thiazolyl blue 86-89 H19 imprinted maternally expressed transcript Homo sapiens 195-198 31938192-6 2018 MTT assay showed that the optical density of the SOCS3 group was significantly higher than that of the control and blank groups. thiazolyl blue 0-3 suppressor of cytokine signaling 3 Rattus norvegicus 49-54 29307194-5 2018 The derivative TP-disulfide-CR7 (TP-S-S-CR7) containing a disulfide linkage between TP and R7 possesses less toxicity at various concentrations on the immortal human keratinocyte (HaCaT) cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay compared with free TP. thiazolyl blue 200-260 teratocarcinoma-derived growth factor 1 pseudogene 7 Homo sapiens 28-31 29307194-5 2018 The derivative TP-disulfide-CR7 (TP-S-S-CR7) containing a disulfide linkage between TP and R7 possesses less toxicity at various concentrations on the immortal human keratinocyte (HaCaT) cell line by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay compared with free TP. thiazolyl blue 200-260 teratocarcinoma-derived growth factor 1 pseudogene 7 Homo sapiens 40-43 29139396-3 2018 No obvious toxicity of the PF127-FA@Ag2S nanoprobe was found in standard 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and colony-formation assay, indicating good biocompatibility and safety. thiazolyl blue 73-135 angiotensin II receptor type 1 Homo sapiens 36-40 29303982-5 2018 In this study, the cytotoxic effects of the curcumin analog DK1 was investigated in both U-2OS and MG-63 osteosarcoma cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell death was microscopically examined via acridine orange/propidium iodide (AO/PI) double staining. thiazolyl blue 135-195 immunoglobulin heavy diversity 5-12 Homo sapiens 60-63 28177128-5 2017 The SGK1 inhibitor SI113 induced a significant reduction of endometrial cancer cells viability, measured by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. thiazolyl blue 113-174 serum/glucocorticoid regulated kinase 1 Homo sapiens 4-8 31938116-5 2018 In CD4+ T cells isolated from SF of active RA patients, LSD1 downregulation significantly increased cell proliferation, as shown by MTT assay. thiazolyl blue 132-135 lysine demethylase 1A Homo sapiens 56-60 30014744-3 2018 Osteosarcoma cell growth affected by miR-149 was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assays. thiazolyl blue 62-122 microRNA 149 Homo sapiens 37-44 29466794-3 2018 METHODS: The effect of FOXO1 gene on cellular function and apoptosis and its underlying mechanisms were investigated in cultured QBC939 cells by the methylthiazoletetrazolium (MTT) assay, western blot, DCFDA mitochondrial membrane potential, and ATP content measurement. thiazolyl blue 149-174 forkhead box O1 Homo sapiens 23-28 29466794-3 2018 METHODS: The effect of FOXO1 gene on cellular function and apoptosis and its underlying mechanisms were investigated in cultured QBC939 cells by the methylthiazoletetrazolium (MTT) assay, western blot, DCFDA mitochondrial membrane potential, and ATP content measurement. thiazolyl blue 176-179 forkhead box O1 Homo sapiens 23-28 31938087-6 2018 The MTT assay showed that cell proliferation was significantly inhibited by AQP-5-siRNA transfection compared to that in NS-siRNA-transfected cells (P < 0.05). thiazolyl blue 4-7 aquaporin 5 Homo sapiens 76-81 31938088-4 2018 MTT assay was performed to detect the SHI-1/CXCR4i cell proliferative rate. thiazolyl blue 0-3 C-X-C motif chemokine receptor 4 Homo sapiens 44-49 30099456-2 2018 METHODS: The MTT (3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was used to detect hLEC -viability. thiazolyl blue 18-80 C-C motif chemokine ligand 16 Homo sapiens 107-111 29345189-12 2018 Results from 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, flow cytometry, and Transwell assay demonstrated that the inhibition of Pax6 expression in MG-63 cells could reverse the cell biological effects induced by the inhibition of miR-19 expression. thiazolyl blue 13-73 paired box 6 Homo sapiens 147-151 28822783-8 2017 Furthermore, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and lactate dehydrogenase (LDH) assays showed that hsa-miR-132-5p attenuates CYP1A2-mediated, lansoprazole-enhanced, flutamide-induced hepatic cell toxicity. thiazolyl blue 13-73 cytochrome P450 family 1 subfamily A member 2 Homo sapiens 158-164 31966378-4 2017 In this article, we examined artemin (ARTN) promoted both cell proliferation and metastasis of human LSCC cells Hep-2 by siRNA mediated ARTN knocking down using MTT assay, cell migration assay and cell invasion assay. thiazolyl blue 161-164 artemin Homo sapiens 29-36 28981607-5 2017 Then, the effects of SLC39A7 knockdown on colorectal cancer cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide, colony-forming assay and flow cytometry. thiazolyl blue 83-145 solute carrier family 39 member 7 Homo sapiens 21-28 28916337-3 2017 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that Ang II induced podocyte death. thiazolyl blue 0-60 angiotensinogen Homo sapiens 85-91 28278681-4 2017 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test showed a dose-dependent viability reduction (r < -0.95; p < 0.01) (range 5-80 muM). thiazolyl blue 0-60 latexin Homo sapiens 156-159 31966378-4 2017 In this article, we examined artemin (ARTN) promoted both cell proliferation and metastasis of human LSCC cells Hep-2 by siRNA mediated ARTN knocking down using MTT assay, cell migration assay and cell invasion assay. thiazolyl blue 161-164 artemin Homo sapiens 38-42 28849232-6 2017 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), bromodeoxyuridine (BrdU), colony formation and cell cycle assays showed that overexpression of miR-330-5p markedly inhibited cell growth. thiazolyl blue 4-64 microRNA 330 Homo sapiens 167-174 28675377-7 2017 Biocompatibilities of the NZF and NZF@Alb NPs were confirmed by in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay against RAW 264.7 cells. thiazolyl blue 73-133 albumin Mus musculus 34-41 28606919-5 2017 Cell proliferation ability and cytotoxicity of inhibiting SOX9 were assessed by 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) and clonogenic assays. thiazolyl blue 80-141 SRY-box transcription factor 9 Homo sapiens 58-62 27689466-9 2017 Meanwhile, down-regulation of ERalpha inhibited E2-induced protective effect on cisplatin toxicity as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. thiazolyl blue 116-176 estrogen receptor 1 Homo sapiens 30-37 28732161-6 2017 The cytotoxicity of MNP-G2/MTX using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide based assay and MCF-7, HeLa, and Caov-4 cell lines revealed that MNP-G2/MTX was more active against the tumor cells than the free drug in a mildly acidic environment. thiazolyl blue 40-100 metaxin 1 Homo sapiens 166-176 27779824-4 2017 Spontaneous and concanavalin A or lipopolysaccharide (LPS)-induced cell proliferation was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric method. thiazolyl blue 102-162 toll-like receptor 4 Mus musculus 54-57 31966711-3 2017 Overexpressed ERbeta significantly inhibited cell proliferation in these two prostate cancer cell lines using MTT assay. thiazolyl blue 110-113 estrogen receptor 1 Homo sapiens 14-20 28758931-7 2017 The effect of the EGFR blockade was assessed by treating the cells with gefitinib, and analyzed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Western Blot (WB). thiazolyl blue 105-165 epidermal growth factor receptor Homo sapiens 18-22 28538837-6 2017 The effect of HDAC2 knockdown on cell proliferation was then assessed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 73-134 histone deacetylase 2 Homo sapiens 14-19 28100780-8 2017 Consistently, TrkC-miR2 overexpression promoted SW480 cell survival, which was detected by flow cytometry, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, and crystal violate analysis. thiazolyl blue 112-172 neurotrophic receptor tyrosine kinase 3 Homo sapiens 14-18 28415822-5 2017 Treatment of human HepG-2 cells with GRN A inhibited cancer cell growth as well as migration and invasion of cancer cells as analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazoliumbromide (MTT) and Scratch wound healing assay as well as Transwell experiments. thiazolyl blue 141-201 granulin precursor Homo sapiens 37-40 28732161-6 2017 The cytotoxicity of MNP-G2/MTX using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide based assay and MCF-7, HeLa, and Caov-4 cell lines revealed that MNP-G2/MTX was more active against the tumor cells than the free drug in a mildly acidic environment. thiazolyl blue 40-100 metaxin 1 Homo sapiens 20-30 31966695-5 2017 Pretreatment with AUDA (0.5-8 microM) dose-dependently inhibited TNF-alpha-induced proliferation of HASMCs as revealed by the MTT assay and the decreased expression of Cyclin D1 and beta-tubulin. thiazolyl blue 126-129 tumor necrosis factor Homo sapiens 65-74 29071036-4 2017 Results: The MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyl tetrazolium bromide) assay showed significant decrease in HGF cell viability in both hand instruments only and combination treated teeth specimens compared to control specimens (P<0.05), 24 hours after cell seeding. thiazolyl blue 18-81 hepatocyte growth factor Homo sapiens 120-123 28691641-8 2017 The cell proliferation was reduced following SPRY4-IT1 overexpression in SKOV3/HO8910 cells based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays. thiazolyl blue 101-161 SPRY4 intronic transcript 1 Homo sapiens 45-54 28485806-5 2017 MATERIALS AND METHODS: The cell proliferation, migration and invasion of SGC-7901 were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, Transwell assay and cell invasion assay. thiazolyl blue 101-162 sarcoglycan beta Homo sapiens 73-76 28488543-4 2017 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry assays demonstrated that transfection with small interfering RNA to silence FOXA1 (si-FOXA1) in ovarian cancer cell lines decreased cell proliferation and induced apoptosis and S-phase arrest. thiazolyl blue 0-60 forkhead box A1 Homo sapiens 156-161 28488543-4 2017 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry assays demonstrated that transfection with small interfering RNA to silence FOXA1 (si-FOXA1) in ovarian cancer cell lines decreased cell proliferation and induced apoptosis and S-phase arrest. thiazolyl blue 0-60 forkhead box A1 Homo sapiens 166-171 28485781-3 2017 After treated by hTERT ASODN the proliferation of the fibroblasts was measured by cell count and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay method, the apoptosis was analyzed by flow cytometry (FCM), and the expression of hTERT and bcl-2 mRNA were observed by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). thiazolyl blue 102-162 telomerase reverse transcriptase Homo sapiens 17-22 28475004-5 2017 Cell viability, colony formation capacity, and phosphorylation of Janus kinase 2 and signal transducer and activator of transcription 3 in treated SGC-7901 and BGC-823 cells were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, colony formation assay, and western blot analysis, respectively. thiazolyl blue 191-251 Janus kinase 2 Homo sapiens 66-135 28243117-5 2017 Using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry, we demonstrated that expression of AIM2 inhibited the viability and increased the apoptosis rate of CRC cells, and cell cycle analysis suggested that AIM2 blocked cell cycle transition from G1 to S phase. thiazolyl blue 6-66 absent in melanoma 2 Homo sapiens 134-138 28462147-6 2017 In vitro anticancer activity on DU-145 and PC-3 human prostate cancer cell lines was evaluated by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assay. thiazolyl blue 99-163 chromobox 8 Homo sapiens 43-47 30108807-2 2017 The cytotoxicity of LIM was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 48-108 PDZ and LIM domain 5 Mus musculus 20-23 28243117-5 2017 Using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry, we demonstrated that expression of AIM2 inhibited the viability and increased the apoptosis rate of CRC cells, and cell cycle analysis suggested that AIM2 blocked cell cycle transition from G1 to S phase. thiazolyl blue 6-66 absent in melanoma 2 Homo sapiens 249-253 27981550-5 2016 The effects of TRIM59 knockdown on cell proliferation were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assays. thiazolyl blue 71-131 tripartite motif-containing 59 Mus musculus 15-21 27780761-5 2017 In vitro inhibition of Cathepsins B, H and L is correlated well with in vitro studies when tested using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay on HepG2 cells, hepatocellular carcinoma cell line. thiazolyl blue 109-169 cathepsin L Homo sapiens 23-44 26758454-5 2017 In this study, we observed that IGF-1 can salvage the hippocampal neurons in an autophagy-independent manner after 150-muM NMDA exposure using thiazolyl blue tetrazolium bromide (MTT), lactate dehydrogenase (LDH), Western blot assay, and transmission electron microscopy. thiazolyl blue 143-177 insulin like growth factor 1 Homo sapiens 32-37 28176933-10 2017 A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytocompatibility assay in Human Foetal Osteoblast Cell Line hFOB 1.19 showed that ANC can safely support osteoblast proliferation up to 48 h whereas PTH-ANC can safely support the proliferation at 72 h and beyond due to the sustained slow release of PTH 1-34. thiazolyl blue 2-62 parathyroid hormone Homo sapiens 213-216 29130985-3 2017 HMF decreased the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by anti-CD3/CD28 antibody-stimulated mouse spleen cells. thiazolyl blue 31-91 CD3 antigen, epsilon polypeptide Mus musculus 106-109 29130985-3 2017 HMF decreased the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by anti-CD3/CD28 antibody-stimulated mouse spleen cells. thiazolyl blue 31-91 CD28 antigen Mus musculus 110-114 27909722-5 2017 Immunosuppressive activity of CD4+CD25- Treg was measured via thiazolyl blue in an MTT assay. thiazolyl blue 62-76 CD4 molecule Homo sapiens 30-33 27909722-5 2017 Immunosuppressive activity of CD4+CD25- Treg was measured via thiazolyl blue in an MTT assay. thiazolyl blue 62-76 interleukin 2 receptor subunit alpha Homo sapiens 34-38 27879679-5 2016 Further, H-TL1 attenuated the cytotoxicity of TNF-alpha in L929 cells as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. thiazolyl blue 91-152 tumor necrosis factor Mus musculus 46-55 27899975-5 2016 In addition, the effects of the isolated LP-15 RCC1 protein"s functional domain on the viability and apoptosis of human lung cancer A549 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, transmission electron microscopy, flow cytometry and Hoechst 33258 staining. thiazolyl blue 160-220 regulator of chromosome condensation 1 Homo sapiens 47-51 27840577-5 2016 However, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) cell proliferation assays demonstrate that the demethylase activity is required for cell viability. thiazolyl blue 9-69 methyl-CpG binding domain protein 2 Homo sapiens 123-134 27159062-4 2016 The viability of the HCE-2 cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 56-116 carboxylesterase 2 Homo sapiens 21-26 27432288-3 2016 Using 3-4,5-dimethylthiazol-2-yl-2,5 diphenyl tetrazolium bromide, wound healing and flow cytometry assays, it was determined that overexpression of miR-199a-3p in Ntera-2 cells caused suppression of cell growth and migration. thiazolyl blue 6-65 microRNA 199a-1 Homo sapiens 149-160 27511117-11 2016 Consistently, overexpression of PIK3CA-premir1 in HCT116 and SW480 cells resulted in significant reduction of the sub-G1 cell distribution and apoptotic cell rate, as detected by flowcytometry, and resulted in increased cell proliferation, as detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 255-315 phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha Homo sapiens 32-46 27433833-7 2016 Moreover, (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay indicates that GGH increased PC-12 cell viability from 36% to 63%, and neurotoxicity partly triggered by ROS decreased. thiazolyl blue 11-72 gamma-glutamyl hydrolase Rattus norvegicus 100-103 27698867-5 2016 H19 siRNA treatment markedly inhibited the proliferation of keloid fibroblasts, as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (P=0.008). thiazolyl blue 95-155 H19 imprinted maternally expressed transcript Homo sapiens 0-3 27431620-6 2016 The biological function of DKK2 in prostate cancer was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and transwell invasion assays. thiazolyl blue 74-134 dickkopf WNT signaling pathway inhibitor 2 Homo sapiens 27-31 27630541-4 2016 Exposure of cultured brain microvascular endothelial cells (bEND3 cells) to oxygen-glucose deprivation (OGD) also induced ENOPH1 upregulation, which was accompanied by increased cell death and apoptosis reflected by increased 3-(4, 5-Dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide formation, lactate dehydrogenase release and TUNEL staining. thiazolyl blue 226-289 enolase-phosphatase 1 Mus musculus 122-128 27174747-4 2016 Incubation of GCs with either rcGH or conditioned media, containing predominantly a 15-kDa GH isoform, showed that both significantly increased proliferation as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, proliferating cell nuclear antigen (PCNA) quantification and ((3)H)-thymidine incorporation ((3)H-T) assays in a dose response fashion. thiazolyl blue 179-239 growth hormone Gallus gallus 32-34 27579889-4 2016 Our results revealed that beta2-GPI dose-dependently decreased the VEGF-induced increase in endothelial cell proliferation, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and the bromodeoxyuridine (BrdU) incorporation assays. thiazolyl blue 134-194 apolipoprotein H Homo sapiens 26-35 27579889-4 2016 Our results revealed that beta2-GPI dose-dependently decreased the VEGF-induced increase in endothelial cell proliferation, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and the bromodeoxyuridine (BrdU) incorporation assays. thiazolyl blue 134-194 vascular endothelial growth factor A Homo sapiens 67-71 27123081-4 2016 The selective killing effect of Ad-VEGFp-CDglyTK was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry (FCM) in vitro and by xenograft studies in vivo. thiazolyl blue 65-125 vascular endothelial growth factor A Homo sapiens 35-40 27349269-4 2016 Functionally, senescence-associated beta-galactosidase staining, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), clonogenic and Transwell assays showed that in addition to acting as a pioneer factor, FOXA1 was significantly upregulated in senescent EC cells. thiazolyl blue 65-125 forkhead box A1 Homo sapiens 220-225 27364790-7 2016 Cell viability was tested after treatment with Lenti-NRARP-shRNA using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 71-131 NOTCH regulated ankyrin repeat protein Homo sapiens 53-58 27287327-8 2016 The 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide and would healing assays, respectively, indicated that the secreted KiSS1 peptide remarkably inhibited HT-29 cell proliferation and migration. thiazolyl blue 4-65 KiSS-1 metastasis suppressor Homo sapiens 134-139 27357419-4 2016 Additionally, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to detect the drug-resistance phenotype changes of cancer cells associated with upregulation or downregulation of miR-20a. thiazolyl blue 14-74 microRNA 20a Homo sapiens 198-205 26865218-3 2016 METHODS AND RESULTS: Coadministration of quercetin and valinomycin (50 muM quercetin/0.05 muM valinomycin) reduced intracellular reactive oxygen species content and increased cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) of Caco-2 cells compared to valinomycin-only (0.05 muM) treatment. thiazolyl blue 191-251 latexin Homo sapiens 71-74 25882880-6 2016 The results of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide and colony formation assay indicated that upregulated fascin expression could enhance cholangiocarcinoma cell proliferation. thiazolyl blue 15-75 fascin actin-bundling protein 1 Homo sapiens 130-136 26254808-8 2015 RESULTS: Ad-shRRM1 effectively downregulated RRM1 mRNA and protein in both types of NSCLC cells and significantly reduced the percentage of viable cells as detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay (p<0.005). thiazolyl blue 168-228 ribonucleotide reductase catalytic subunit M1 Homo sapiens 14-18 26633557-4 2015 The effects of FAT4 silencing on cell proliferation, migration, and invasion were assessed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) assay, and migration and invasion assays in gastric cancer cell lines in vitro and in a mouse xenograft model in vivo. thiazolyl blue 103-155 FAT atypical cadherin 4 Mus musculus 15-19 26715855-7 2015 Furthermore, we overexpressed NRBP1 in MCF-7 and MDA-MB-231 breast cancer cell lines and found NRBP1 upregulation-inhibited cell proliferation by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 152-212 nuclear receptor binding protein 1 Homo sapiens 30-35 26715855-7 2015 Furthermore, we overexpressed NRBP1 in MCF-7 and MDA-MB-231 breast cancer cell lines and found NRBP1 upregulation-inhibited cell proliferation by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 152-212 nuclear receptor binding protein 1 Homo sapiens 95-100 27022281-9 2016 We experimentally verified that one of these novel agents, prenylamine, induced HCC cell apoptosis using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, an acridine orange/ethidium bromide stain, and electron microscopy. thiazolyl blue 105-165 HCC Homo sapiens 80-83 26998071-4 2016 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) results revealed that the proliferation of MKN28 cells was notably increased following upregulation of the expression of miR-196b (P<0.01). thiazolyl blue 0-60 microRNA 196b Homo sapiens 188-196 26708577-6 2016 The functional impact of miR-20b-5p on cell proliferation, cell migration and apoptosis in the 786-O and ACHN RCC cell lines, was determined by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, a scratch assay and flow cytometry. thiazolyl blue 147-207 microRNA 20b Homo sapiens 25-32 27725440-4 2016 Interestingly, Mrp3 siRNA or probenecid treatment also resulted in increased cytotoxicity, as evidenced by decreased 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-reducing activity of Neuro2a, with a concomitant increase in release of lactate dehydrogenase. thiazolyl blue 117-177 prolactin family 2, subfamily c, member 4 Mus musculus 15-19 29023018-7 2016 The barriers to proliferation through the test of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, barriers to invasion through Boyden chamber assay and the membrane polycarbonate, ELISA analysis and Western blotting analysis on the obstacle Akt signal transduction pathways and transcription factor of nuclear factor-kappa B (NF-kappaB). thiazolyl blue 55-115 AKT serine/threonine kinase 1 Homo sapiens 267-270 29023018-10 2016 RESULTS: This study showed that the average cell growth inhibition SP-C1 based on the time and concentration using the MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. thiazolyl blue 124-185 signal peptidase complex subunit 1 Homo sapiens 67-72 26269116-7 2016 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and EdU approaches showed that CDX2 overexpression markedly suppressed pancreatic adenocarcinoma cell proliferation. thiazolyl blue 0-60 caudal type homeobox 2 Mus musculus 98-102 26463665-7 2015 The TNF-mediated chemosensitization was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay. thiazolyl blue 54-114 tumor necrosis factor Homo sapiens 4-7 26176363-4 2015 Under challenge with H2O2, loss of cell viability was observed in cultured rat NSCs, and treatment with selective mGluR4 agonist VU0155041 conferred protective effects against the loss of cellular viability in a concentration-dependent manner, as shown by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. thiazolyl blue 256-317 glutamate receptor, ionotropic, AMPA4 (alpha 4) Mus musculus 114-120 26252641-6 2015 The proliferation of gastric cancer cells was significantly inhibited in TCTN1 knockdown cells, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays. thiazolyl blue 113-173 tectonic family member 1 Homo sapiens 73-78 26410776-8 2015 Furthermore, the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide) assay established the cytotoxicity of the glycated- HSA towards human liver carcinoma (HepG2) cell lines via the production of reactive oxygen species. thiazolyl blue 22-85 albumin Homo sapiens 139-142 26198640-6 2015 Similarly, results from a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed a larger reduction of viability of C4-2-EV cells compared with C4-2-DN cells when treated with TRAIL-TZD, thus suggesting that C4-2-DN cells were more apoptosis-resistant. thiazolyl blue 26-86 TNF superfamily member 10 Homo sapiens 195-200 26165988-9 2015 The 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and colony formation assay showed that ECRG4 overexpression suppressed the proliferative capacity of laryngeal cancer cells in vitro. thiazolyl blue 4-65 ECRG4 augurin precursor Homo sapiens 111-116 25983001-3 2015 PrP(C) overexpression was shown to increase cell proliferation in anchorage-dependent and anchorage-independent manners, as shown by more viable cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, more colonies formed in soft agar assay and increased resistance to anoikis in poly-2-hydroxyethyl methacrylate-coated surface. thiazolyl blue 154-214 prion protein Homo sapiens 0-6 25833696-3 2015 In this study, we used specific si-RNA to downregulate RPS15A expression in A549 cells and found that decreased RPS15A expression significantly inhibited cell proliferation and survival, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays. thiazolyl blue 204-264 ribosomal protein S15a Homo sapiens 112-118 25179429-6 2015 HEYL expressional analysis and functional characterization were assessed by 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyltetrazolium bromide assays, flow cytometry, RT-qPCR, western blotting and methylation-specific PCR. thiazolyl blue 76-136 hes related family bHLH transcription factor with YRPW motif like Homo sapiens 0-4 26305684-5 2015 For this purpose, H9C2 cells were treated with recombinant irisin produced in yeast cells (r-irisin) and in HEK293 cells (hr-irisin) for examining its effects on cell proliferation by MTT [3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and on gene transcription profiles by qRT-PCR. thiazolyl blue 189-250 fibronectin type III domain containing 5 Homo sapiens 59-65 26157144-6 2015 NR4A1 overexpression in MIN6 cells conferred resistance to cell loss induced by TG or PA, as assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, and TUNEL assays indicated that NR4A1 overexpression also protected against ER stress-induced apoptosis. thiazolyl blue 110-170 nuclear receptor subfamily 4, group A, member 1 Mus musculus 0-5 26271217-10 2015 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that PGFS were non-cytotoxic against bone marrow stromal cells (bMSCs) after culture for up to 72 h. These results suggest that PGFS could be promising scaffolds for bone regeneration applications. thiazolyl blue 5-65 aldo-keto reductase family 1 member C3 Homo sapiens 88-92 26271217-10 2015 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that PGFS were non-cytotoxic against bone marrow stromal cells (bMSCs) after culture for up to 72 h. These results suggest that PGFS could be promising scaffolds for bone regeneration applications. thiazolyl blue 5-65 aldo-keto reductase family 1 member C3 Homo sapiens 211-215 26205793-5 2015 FasL treatment to HeLa cells resulted in a concentration-dependent induction of cell death, and treatment with low concentrations of DC (0.1-2 microg/mL) significantly (p < 0.001) attenuated the FasL-induced cell death as measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 237-297 Fas ligand Homo sapiens 0-4 26205793-5 2015 FasL treatment to HeLa cells resulted in a concentration-dependent induction of cell death, and treatment with low concentrations of DC (0.1-2 microg/mL) significantly (p < 0.001) attenuated the FasL-induced cell death as measured by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 237-297 Fas ligand Homo sapiens 198-202 25815776-7 2015 Functional investigations demonstrated that the overexpression of miR-509-3p inhibited the migration and proliferation of the RCC cells, as determined by wound scratch and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. thiazolyl blue 172-232 microRNA 5093 Homo sapiens 66-76 25920913-5 2015 The effects of RNAi-mediated CDKL1 downregulation on A375 and MV3 cell proliferation and colony-formation ability were detected by methylthiazoletetrazolium (MTT) assay and colony-formation assay. thiazolyl blue 131-156 cyclin dependent kinase like 1 Homo sapiens 29-34 25920913-5 2015 The effects of RNAi-mediated CDKL1 downregulation on A375 and MV3 cell proliferation and colony-formation ability were detected by methylthiazoletetrazolium (MTT) assay and colony-formation assay. thiazolyl blue 158-161 cyclin dependent kinase like 1 Homo sapiens 29-34 25738567-5 2015 A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay demonstrated that the viability of human microglia and oligodendrocytes were significantly reduced in a time- and dose-dependent manner following treatment with the ADAM17 antagonist, TNF protease inhibitor 2. thiazolyl blue 2-62 ADAM metallopeptidase domain 17 Homo sapiens 234-240 25645193-2 2015 METHODS: The protection and underlying mechanisms were detected in CYP2E1 overexpression primary rat hepatocytes by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, alamar blue assay, CYP2E1 inhibition assay and glutathione assay. thiazolyl blue 116-176 cytochrome P450, family 2, subfamily e, polypeptide 1 Rattus norvegicus 67-73 25962180-4 2015 The biological functions of miR-138 and Bag-1 (Bcl-2-associated athanogene-1) on cell proliferation were examined using 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide and apoptosis assays. thiazolyl blue 120-180 BCL2-associated athanogene 1 Mus musculus 47-76 26125866-6 2015 The effects of overexpression of PTEN and KRT10 on cisplatin resistance of ovarian cancer cells were examined using the 3(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) and TdT-mediated dUTP nick-end labeling (TUNEL) assays in vitro. thiazolyl blue 120-178 phosphatase and tensin homolog Homo sapiens 33-37 25672943-8 2015 Cell proliferation was evaluated by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay at 0, 12, 24, 48 and 72 h. The results demonstrated that A2BR was the predominant AR subtype present in hypoxia-stimulated fibroblasts. thiazolyl blue 38-98 adenosine A2b receptor Mus musculus 162-166 25967609-3 2015 Moreover, cytotoxicity of aggregated insulin was monitored on SH-SY5Y cell line in the presence and absence of black seeds extract using standard 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) and reactive oxygen species (ROS) assay kit. thiazolyl blue 146-206 insulin Homo sapiens 37-44 25652471-5 2015 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and annexin V/propidium iodide (PI) staining assays demonstrated that evodiamine or MTDH short hairpin RNA (shRNA) significantly inhibited proliferation of A549 cells via induction of apoptosis. thiazolyl blue 0-61 metadherin Homo sapiens 152-156 25836616-5 2015 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to examine changes in the viability of HepG2, Bel-7402, Bel-7404 hepatoma cell lines associated with up-regulation or down-regulation of miR-27a. thiazolyl blue 0-60 microRNA 27a Homo sapiens 207-214 25947066-4 2015 FASN inhibition"s impact on cell viability, apoptosis, and fatty acid metabolism was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide assay, cell death detection enzyme-linked immunosorbent assay, immunoblotting, and (18) F-fluoromethylcholine uptake measurement, respectively. thiazolyl blue 97-157 fatty acid synthase Homo sapiens 0-4 25659740-4 2015 Furthermore, the Cu(II) and Co(II) complexes showed photocytotoxicity toward two selected tumor cell lines MCF-7 and A549 by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) method, and the Cu(II) complex exhibits higher photocytotoxicity than Co(II) complex against each of the selected cell lines, this result is identical with their DNA binding ability order. thiazolyl blue 125-184 mitochondrially encoded cytochrome c oxidase II Homo sapiens 28-34 25798266-3 2015 The viability of the SCC-4 and SCC-25 OSCC cell lines was assessed by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). thiazolyl blue 95-155 MAU2 sister chromatid cohesion factor Homo sapiens 21-26 25542240-2 2015 In the present study, in vitro growth inhibition (8-23 %) at different concentrations (6-24 mug/ml) of MCL was observed against Ehrlich ascites carcinoma (EAC) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 169-229 C-type lectin domain family 4, member d Mus musculus 103-106 25613063-7 2015 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that the ARNT2 overexpression inhibited cell viability, while ARNT2 knockdown promoted cell growth in NSCLC cell lines HCC827 and A549. thiazolyl blue 0-60 aryl hydrocarbon receptor nuclear translocator 2 Homo sapiens 89-94 25367796-7 2015 On one hand, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assay demonstrated that inhibition of Bag5 exacerbated Abeta1-42-related cell death. thiazolyl blue 13-74 BCL2-associated athanogene 5 Mus musculus 151-155 26501801-6 2015 Our previously synthesized benzoxazole (XT2B) and benzamide (XT5) derivatives were tested with in vitro 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, which revealed significant inhibitory activity on PAR1. thiazolyl blue 104-164 coagulation factor II thrombin receptor Homo sapiens 229-233 25234195-5 2015 Results from the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay showed that Ang II induced podocyte death in a concentration-dependent manner. thiazolyl blue 17-79 angiotensinogen Homo sapiens 104-110 25550793-5 2014 Using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) to detecting the influence of miR-29 on the increased proliferation caused by TGF-beta1 on the human orbital fibroblasts. thiazolyl blue 6-68 transforming growth factor beta 1 Homo sapiens 153-162 25377251-5 2014 Using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay and Trypan blue exclusion assay, Rcan1-1L overexpression was found to markedly reverse cell growth inhibition induced by hypoxia. thiazolyl blue 10-70 regulator of calcineurin 1 Homo sapiens 114-119 24526410-4 2014 Here we report that the silencing of GRP78 expression affects both cell growth and invasiveness of Ishikawa and AN3CA cells, analyzed by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and transwell migration assay, respectively. thiazolyl blue 142-203 heat shock protein family A (Hsp70) member 5 Homo sapiens 37-42 25170107-13 2014 ABC-11 cells were sensitive to crizotinib and next-generation ALK inhibitors (ceritinib and AP26113), as determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 121-181 ALK receptor tyrosine kinase Homo sapiens 62-65 24927112-4 2014 By using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), migration and gelatin sponge-chorioallantoic membrane assays, it was demonstrated that when miR-137 was overexpressed, cell viability and migration decreased. thiazolyl blue 13-73 microRNA 137 Homo sapiens 173-180 24833088-7 2014 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony formation assay showed that diversin knockdown inhibited glioma cell growth. thiazolyl blue 0-60 ankyrin repeat domain 6 Homo sapiens 106-114 25009600-4 2014 Furthermore, ERalpha expression in differentiating 3T3-L1 cells was significantly inhibited by 48 h treatment with 50 muM genistein, which was selected based on the results of cytotoxicity assays on 3T3-L1 preadipocytes [lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assays]. thiazolyl blue 253-313 estrogen receptor 1 (alpha) Mus musculus 13-20 24924414-5 2014 Effect of miR-204 on cell viability of SGNs was first examined using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. thiazolyl blue 74-135 microRNA 204 Homo sapiens 10-17 25183280-3 2014 Then, interleukin-1beta and interferon-gamma induced cell death and insulin secretion were measured in rat insulinoma (RIN) cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and ELISA kit, respectively. thiazolyl blue 133-193 interferon gamma Rattus norvegicus 28-44 25331474-4 2014 The effects of Klotho proteins on proliferation, migration and adhesiveness of HUVEC were determined by thiazolyl blue tetrazolium bromide (MTT), scratches, Transwell and cell adhesion assays respectively. thiazolyl blue 104-138 klotho Homo sapiens 15-21 24851048-7 2014 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that BSA-LTB NPs exhibited higher cytotoxic activity than non-targeted NPs (BSA NPs) in SMMC-7721 cells. thiazolyl blue 4-64 lymphotoxin beta Homo sapiens 93-96 24940057-5 2014 The results of the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and confocal laser scanning microscopy indicate that the MSN@Alg microspheres were successfully uptaken by HepG2 without apparent cytotoxicity. thiazolyl blue 19-80 moesin Homo sapiens 150-153 24691657-3 2014 The apoptotic effects of Unc5D in SK-N-BE (p53-/-) and SH-SY5Y (p53+/+) cells were measured by an 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide solution assay. thiazolyl blue 98-157 unc-5 netrin receptor D Homo sapiens 25-30 24650096-4 2014 The molecular mechanisms of ENO1 expression and its effects on cell growth, migration and invasion were also explored by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, Transwell chamber assay, Boyden chamber assay, Western blot and in vivo tumorigenesis in nude mice. thiazolyl blue 121-182 enolase 1, alpha non-neuron Mus musculus 28-32 25063057-5 2014 RESULTS: Anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles significantly inhibited the proliferation of Bel7402 in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay while had almost no effects on L02 cells. thiazolyl blue 115-175 kinase insert domain receptor Homo sapiens 14-21 25063057-5 2014 RESULTS: Anti-VEGFR-2 ScFv-As2O3-stealth nanoparticles significantly inhibited the proliferation of Bel7402 in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay while had almost no effects on L02 cells. thiazolyl blue 115-175 immunglobulin heavy chain variable region Homo sapiens 22-26 24750314-7 2014 The effect of serpin B1 on HaCaT proliferation was analyzed by RNA interference experiments, methylthiazoletetrazolium assay and flow cytometry. thiazolyl blue 93-118 serpin family B member 1 Homo sapiens 14-23 24361215-7 2014 Furthermore, the effect of human DNA methyltransferase 1 knockdown on proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 101-161 DNA methyltransferase 1 Homo sapiens 33-56 24754348-5 2014 The indirect cytotoxicity evaluation based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated that both the neat and the nAg-embedded PVP hydrogels were non-toxic to mouse fibroblasts (L929). thiazolyl blue 46-106 neuroblastoma amplified sequence Mus musculus 150-153 25206849-3 2014 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that transient axonal glycoprotein-1 did not inhibit the proliferation of U251 cells, but promoted cell viability. thiazolyl blue 0-60 contactin 2 Homo sapiens 79-110 24553109-4 2014 In vitro, infection with Ad-hTERT-E1a-HN could inhibit the growth of EC-109 cells significantly and also protect normal human liver cell line L02 from growth suppression in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. thiazolyl blue 173-233 telomerase reverse transcriptase Homo sapiens 28-33 25533827-7 2014 The cytotoxicity of PYY toward the MiaPaCa-2 cell was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; the cell morphology and structure changes were observed under inverted microscope and transmission electron microscope respectively. thiazolyl blue 67-127 peptide YY Homo sapiens 20-23 24485831-4 2014 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted at 24 hours to quantify SCC4 cell survival rates after MTX-ALA-PDT treatment. thiazolyl blue 4-64 MAU2 sister chromatid cohesion factor Homo sapiens 109-113 24401476-5 2014 Furthermore, by transwell migration, invasion assays, and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, we found that exogenous CCL17 concentrations paralleled the invasive potential of cytotrophoblasts to some extent, with no significant effect on cell proliferation. thiazolyl blue 62-122 C-C motif chemokine ligand 17 Homo sapiens 154-159 25605145-8 2014 Consistently, MRP1-silent cells exhibited decreased resistance to 3- (4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and DDP, suggesting reversal of MDR in these tumor cells. thiazolyl blue 66-129 ATP binding cassette subfamily C member 1 Homo sapiens 14-18 24368600-4 2013 Using reverse transcription polymerase chain reactions and western blots, we found that overexpression of CUL4A in MCF7 and MDA-MB-468 cells up-regulated MDR1/P-gp expression on both the transcription and protein levels, which conferred multidrug resistance to P-gp substrate drugs, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. thiazolyl blue 300-360 cullin 4A Homo sapiens 106-111 23777252-6 2013 In addition, a plasmid expressing the RhoBTB2 gene was transfected into human osteosarcoma (HOS) cell using Lipofectamine 2000, and the effects of RhoBTB2 on HOS cell were investigated using 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazoliumbromide assay and flow cytometry. thiazolyl blue 191-247 Rho related BTB domain containing 2 Homo sapiens 38-45 24026460-3 2013 Neuron viability, apoptosis and caspase-8 and -3 activities were assessed by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 4",6-diamidino-2-phenylindole (DAPI) and terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL) and spectrophotometric assays, respectively. thiazolyl blue 94-154 caspase 8 Rattus norvegicus 32-48 24038138-5 2013 Exogenously expressed Prg1 rescued the cells from death and restored the loss of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) activity induced with Prg1 siRNA. thiazolyl blue 81-141 phospholipid phosphatase related 4 Rattus norvegicus 22-26 24038138-5 2013 Exogenously expressed Prg1 rescued the cells from death and restored the loss of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) activity induced with Prg1 siRNA. thiazolyl blue 81-141 phospholipid phosphatase related 4 Rattus norvegicus 170-174 23160374-8 2013 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay conducted with breast cancer cell lines (MCF-7 and T47D) demonstrated that sumoylation of CLOCK stimulated cell growth and increased the proportion of S phase cells in the cell cycle. thiazolyl blue 0-60 clock circadian regulator Homo sapiens 163-168 24222249-13 2013 Human laryngeal carcinoma Hep-2 cells treated with 0.3125-10 microg/mL RPL24 for 24 h displayed significant cell growth inhibition (P < 0.05; N = 6) in assays using 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide compared with that in control (untreated) cells. thiazolyl blue 168-229 ribosomal protein L24 Homo sapiens 71-76 23765166-6 2013 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide results also showed that VEGF significantly enhanced the IC50 of the cells treated with adriamycin. thiazolyl blue 0-61 vascular endothelial growth factor A Homo sapiens 87-91 23877317-5 2013 MTT assay [(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] was used to evaluate the growth inhibition induced by TGM2 inhibitor. thiazolyl blue 12-72 transglutaminase 2, C polypeptide Mus musculus 128-132 24023832-6 2013 The effect of c-fos protein expression was examined in transfection experiments and in a 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide cell viability assay. thiazolyl blue 89-151 Fos proto-oncogene, AP-1 transcription factor subunit Homo sapiens 14-19 23907512-8 2013 Thiazolyl blue tetrazolium bromide assay, Hoechst33342/Propidium Iodide staining and Western blot assay showed that Tat-CRMP2 pretreatment increased cell viability compared with the control group against NMDA exposure by decreasing the cleavage of CRMP2. thiazolyl blue 0-34 tyrosine aminotransferase Homo sapiens 116-119 23907512-8 2013 Thiazolyl blue tetrazolium bromide assay, Hoechst33342/Propidium Iodide staining and Western blot assay showed that Tat-CRMP2 pretreatment increased cell viability compared with the control group against NMDA exposure by decreasing the cleavage of CRMP2. thiazolyl blue 0-34 dihydropyrimidinase like 2 Homo sapiens 120-125 23636893-3 2013 LIF (0.5 and 1 ng/ml) increased PC12 cell viability and significantly attenuated AMA-induced cell death as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 123-183 LIF, interleukin 6 family cytokine Rattus norvegicus 0-3 23553940-7 2013 Knockdown of Srxn1 resulted in decreased cell viability and increased cellular damage as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and lactate dehyrogenase analysis, respectively. thiazolyl blue 103-164 sulfiredoxin 1 Rattus norvegicus 13-18 23913382-6 2013 A 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide assay demonstrated that IL-6/IL-2 can promote lymphocyte proliferation and differentiation. thiazolyl blue 2-62 interleukin-6 Ailuropoda melanoleuca 87-91 23913382-6 2013 A 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide assay demonstrated that IL-6/IL-2 can promote lymphocyte proliferation and differentiation. thiazolyl blue 2-62 interleukin-2 Ailuropoda melanoleuca 92-96 23591743-3 2013 Hepa1-6 cells were treated with 0 to 1,000-microg/ml concentrations of Trx-JZTX-III; this was demonstrated to affect cell viability, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide (MTT) assay. thiazolyl blue 154-215 thioredoxin 1 Mus musculus 71-74 23596351-7 2013 The impact of miR-141 re-expression on 769-P cells was analyzed using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and colony-forming assay. thiazolyl blue 70-130 microRNA 141 Homo sapiens 14-21 23886271-3 2013 The effects of COX-2 silencing on the proliferation, cell cycle and migration of SaOS2 cells were assessed by thiazolyl blue tetrazolium bromide, flow cytometry and migration assays respectively. thiazolyl blue 110-144 mitochondrially encoded cytochrome c oxidase II Homo sapiens 15-20 23291272-10 2013 In function, flow cytometry, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, 5-ethynyl-2"-deoxyuridine (EdU) incorporation assays and animal transplantation assays demonstrated that HBXIP-enhanced cell proliferation of breast cancer through upregulating LMO4 in vitro and in vivo. thiazolyl blue 29-89 late endosomal/lysosomal adaptor, MAPK and MTOR activator 5 Homo sapiens 196-201 28920323-8 2013 The cell cycles, apoptosis, and proliferation of the nm23-H1 transfactant were also detected by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT assay) and flow cytometry (FCM). thiazolyl blue 96-156 NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens 53-60 23073851-4 2013 The influence of miR-203 on the viability of 95-D lung cancer cells was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 88-149 microRNA 203a Homo sapiens 17-24 21429770-6 2013 The biological effects of siRNA-PSCA on PC-3M cells were investigated by examining the cell proliferation through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell cycle distribution through flow cytometry, and migration and invasion potencies through transwell invasion assay upon the PSCA silencing. thiazolyl blue 114-174 prostate stem cell antigen Homo sapiens 32-36 23121049-6 2013 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability and lactate dehydrogenase (LDH) cell death assays revealed that GPR37 was protective against all three toxins in differentiated cells. thiazolyl blue 0-60 G protein-coupled receptor 37 Homo sapiens 146-151 23660215-5 2013 The inhibition rates on proliferation of CIK cells and Cur associated with CIK cells after addition of FasL monoclonal antibody were detected by (thiazolyl blue tetrazolium bromide) MTT. thiazolyl blue 146-180 Fas ligand Homo sapiens 103-107 23164231-3 2013 Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, we observed that the stimulating osteoblast proliferation activity of LF in vitro decreased with increasing iron saturation level at 100 and 1,000 mug/mL. thiazolyl blue 10-70 lactotransferrin Mus musculus 154-156 23560043-5 2013 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that 200 microg/mL PAb inhibits the proliferation of ARH-77 cells by over 50% within 48 h. Flow cytometric assay indicated that PAb treatment significantly increases the number of apoptotic cells compared with other treatments (52.1% vs. NS, 7.3% or control rabbit IgG, 9.9%). thiazolyl blue 0-60 low density lipoprotein receptor adaptor protein 1 Homo sapiens 127-130 23807847-3 2013 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry analysis showed that CTD-NSVs could significantly enhance in vitro toxicity against human breast cancer cell line MCF-7 and induce more significant cell-cycle arrest in G0/G1 phase. thiazolyl blue 0-60 CTD Homo sapiens 107-110 22627469-8 2013 Moreover, cells treated with agTTR exhibited decreases in metabolic activity relative to TTR- or non-TTR-treated cells (p < 0.05) as assessed by reduction of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). thiazolyl blue 166-226 transthyretin Homo sapiens 31-34 22430047-7 2012 Through an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, the drug designated as J-4 had no obvious cytotoxicity in vitro. thiazolyl blue 11-71 immunoglobulin kappa joining 4 Homo sapiens 102-105 23000305-5 2012 HK2 cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. thiazolyl blue 35-96 hexokinase 2 Homo sapiens 0-3 23181744-5 2012 Ku80-specific siRNA or control scramble siRNA was transfected into A549/DDP cells, then cell sensitivity to cisplatin was examined by 3-(4,5-dimethylthia-zol-2-yl)-2,5-diphenyltetrazolium bromide assay and apoptosis was assessed by flow cytometric analysis. thiazolyl blue 134-195 X-ray repair cross complementing 5 Homo sapiens 0-4 22510844-9 2012 Surprisingly, changes to EGFR expression affected cell proliferation activities less significantly as determined by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, an anchorage-independent growth assay, and cell cycle analysis. thiazolyl blue 118-178 epidermal growth factor receptor Homo sapiens 25-29 22462603-9 2012 3-(4,5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay showed reduced spore viability when incubated in E8-tap1 fruit extracts. thiazolyl blue 0-61 suberization-associated anionic peroxidase 1 Solanum lycopersicum 120-124 22791813-8 2012 The resulting increase in catecholamine production was associated with significant inductions in the phosphorylation of signaling proteins ERK, CREB, Src and AKT, upregulated protein expression of nAChR subunits alpha3, alpha4, alpha5 and alpha7 and increased responsiveness to nicotine in 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide and cell migration assays. thiazolyl blue 290-352 mitogen-activated protein kinase 1 Homo sapiens 139-142 22761458-7 2012 The cell viability and apoptosis in ESC treated with MIS was measured by methylthiazoletetrazolium assay and annexin V analysis. thiazolyl blue 73-98 anti-Mullerian hormone Homo sapiens 53-56 22373584-3 2012 The alterations of the biological characteristics of RKIP-transfected MDA-MB-231 cells were analyzed using the following approaches: a growth curve, a 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) assay, bromodeoxyuridine (BrdU) staining and a cell migration assay. thiazolyl blue 151-211 phosphatidylethanolamine binding protein 1 Homo sapiens 53-57 22328001-6 2012 The impact of CIP2A on proliferation and drug resistance was evaluated using the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide test. thiazolyl blue 81-142 cellular inhibitor of PP2A Homo sapiens 14-19 22270613-6 2012 The result of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay indicated that LIF protected podocytes against high glucose-induced cytotoxicity. thiazolyl blue 14-74 LIF interleukin 6 family cytokine Homo sapiens 96-99 22551380-4 2012 The effect of Klotho protein was examined with transfection experiments, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, senescence-associated beta-galactosidase staining, and cell-cycle analysis. thiazolyl blue 73-133 Klotho Rattus norvegicus 14-20 22411957-10 2012 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide and wound healing assay revealed that knockdown of HOXA10 in GC cells by short interfering RNA transfection significantly increased viability and motility relative to the negative control, indicating that HOXA10 expression inhibits cell growth and motility. thiazolyl blue 0-62 homeobox A10 Homo sapiens 114-120 22753710-3 2012 MATERIALS AND METHODS: In vitro study: Huh7 proliferation was assayed by 3H-thymidine incorporation and by thiazolyl blue tetrazolium bromide (MTT) assay. thiazolyl blue 107-141 MIR7-3 host gene Homo sapiens 39-43 22411704-6 2012 Rhotekin knock-down reduced 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) activity and increased the cell death 72 hr after transfection in neurons. thiazolyl blue 28-88 rhotekin Homo sapiens 0-8 21902544-4 2012 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was employed to measure the cellular proliferation after treatment with various concentrations of recombinant human VEGF-C (rhVEGF-C). thiazolyl blue 4-64 vascular endothelial growth factor C Homo sapiens 187-193 22344630-7 2012 MTT assay showed negative correlation between MIS exposure time and the viability of cervical cells (P=0.008). thiazolyl blue 0-3 anti-Mullerian hormone Homo sapiens 58-61 22142686-4 2012 Also, in vitro assays of proliferation, migration and expression of matrix metalloproteinase-2 (MMP-2) in human aortic smooth muscle cells (HASMCs) were carried out using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, transwell boyden chamber method and gelatin zymography, respectively. thiazolyl blue 171-231 matrix metallopeptidase 2 Homo sapiens 96-101 22417743-7 2012 Proliferative effect of human recombinant IL-22 (rIL-22) on FLS was assessed by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a yellow tetrazole) and CFSE dilution (Carboxyfluorescein succinimidyl ester) assays. thiazolyl blue 85-147 interleukin 22 Homo sapiens 42-47 22417743-7 2012 Proliferative effect of human recombinant IL-22 (rIL-22) on FLS was assessed by MTT (3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a yellow tetrazole) and CFSE dilution (Carboxyfluorescein succinimidyl ester) assays. thiazolyl blue 85-147 interleukin 22 Rattus norvegicus 49-55 21822301-5 2012 The effect of miR-335 expression on gastric cancer cell proliferation was estimated by the 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay. thiazolyl blue 91-152 microRNA 335 Homo sapiens 14-21 22430528-5 2012 Pretreatment with XG significantly attenuated the cell viability reduction induced by Abeta exposure in a dose dependent manner which was testified by 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase release assay. thiazolyl blue 151-211 amyloid beta precursor protein Homo sapiens 86-91 20594620-5 2012 Further study revealed that Abeta oligomer even at 1 muM accelerated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) formazan exocytosis in mature oligodendrocytes, and, more significantly, inhibited myelin sheet formation after induction of in vitro differentiation of OPCs. thiazolyl blue 69-129 amyloid beta precursor protein Rattus norvegicus 28-33 22002459-6 2012 Treatment with the cytohesin inhibitor SecinH3 or with an ARNO siRNA prevented VEGF-dependent Akt activation, assessed by detection of phosphorylated Akt, and proliferation of endothelial cells in vitro, measured by methylthiazoletetrazolium (MTT) reduction. thiazolyl blue 216-241 cytohesin 2 Mus musculus 58-62 22002459-6 2012 Treatment with the cytohesin inhibitor SecinH3 or with an ARNO siRNA prevented VEGF-dependent Akt activation, assessed by detection of phosphorylated Akt, and proliferation of endothelial cells in vitro, measured by methylthiazoletetrazolium (MTT) reduction. thiazolyl blue 216-241 vascular endothelial growth factor A Mus musculus 79-83 22002459-6 2012 Treatment with the cytohesin inhibitor SecinH3 or with an ARNO siRNA prevented VEGF-dependent Akt activation, assessed by detection of phosphorylated Akt, and proliferation of endothelial cells in vitro, measured by methylthiazoletetrazolium (MTT) reduction. thiazolyl blue 243-246 cytohesin 2 Mus musculus 58-62 22002459-6 2012 Treatment with the cytohesin inhibitor SecinH3 or with an ARNO siRNA prevented VEGF-dependent Akt activation, assessed by detection of phosphorylated Akt, and proliferation of endothelial cells in vitro, measured by methylthiazoletetrazolium (MTT) reduction. thiazolyl blue 243-246 vascular endothelial growth factor A Mus musculus 79-83 22561491-3 2012 The effects of SPATA12 on tumor cell proliferation and colony formation was detected by 3-(4.5-dimethylthiazol-2- yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and colonyforming assays, respectively. thiazolyl blue 88-150 spermatogenesis associated 12 Homo sapiens 15-22 22052201-7 2012 All results from the (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) MTT assay, the proliferation curve assay and the soft agar colony formation assay showed that miR-483 promoted the proliferation of hepatocellular carcinoma cells. thiazolyl blue 21-82 microRNA 483 Mus musculus 178-185 22694166-2 2012 In this study, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to investigate the growth inhibitory effect of 16-DHP. thiazolyl blue 15-75 dihydropyrimidinase Homo sapiens 141-144 21947487-3 2011 The results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays showed that miR-191 could promote cell growth and suppress apoptosis of MGC803 cells. thiazolyl blue 15-75 microRNA 191 Homo sapiens 116-123 21963769-8 2012 Methylthiazoletetrazolium (MTT) assay showed that the purified LL-37-haFGF also had a distinct mitogenic activity in NIH 3T3 cells. thiazolyl blue 0-25 cathelicidin antimicrobial peptide Homo sapiens 63-68 21963769-8 2012 Methylthiazoletetrazolium (MTT) assay showed that the purified LL-37-haFGF also had a distinct mitogenic activity in NIH 3T3 cells. thiazolyl blue 27-30 cathelicidin antimicrobial peptide Homo sapiens 63-68 21956400-7 2012 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide dye assay revealed that HNSCC cell proliferation increased upon stimulation of the cells with CypA, whereas cisplatin-induced cell death decreased after stimulation. thiazolyl blue 4-76 peptidylprolyl isomerase A Homo sapiens 183-187 21706234-8 2011 Walnut extract reduced Abeta-mediated cell death assessed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction, and release of lactate dehydrogenase (membrane damage), DNA damage (apoptosis) and generation of ROS in a concentration-dependent manner. thiazolyl blue 66-126 amyloid beta precursor protein Rattus norvegicus 23-28 21920637-6 2011 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the in vitro antitumor effects of the SCP 1 on human breast cancer cell line, T-47D. thiazolyl blue 4-64 synaptonemal complex protein 1 Homo sapiens 137-142 22340175-5 2011 METHODS: AEG-1 was ectopically overexpressed in breast cancer MCF-7 cells and its biological effects on the proliferation and invasion of MCF-7 cells were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and invasion assays. thiazolyl blue 166-226 metadherin Homo sapiens 9-14 21107573-3 2011 METHODS: The GGTI-treated cells were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, flow cytometric analysis, transwell chamber assays, and immunofluorescent staining. thiazolyl blue 49-110 protein geranylgeranyltransferase type I subunit beta Homo sapiens 13-17 21834715-6 2011 The effect of MED19 shRNA on Aspc-1 and Panc-1 cell proliferation was evaluated by methylthiazoletetrazolium assay, BrdU incorporation assay, colony formation assay, and flow cytometry assay. thiazolyl blue 83-108 mediator complex subunit 19 Homo sapiens 14-19 21777146-5 2011 In gain-of-function experiments, the authors found that hsa-miR-125a-5p suppressed proliferation and induced apoptosis in A549 cells by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and flow cytometry, respectively. thiazolyl blue 141-201 microRNA 125a Homo sapiens 60-68 21959454-3 2011 Tests used included a cell viability test, an alkaline phosphatase activity test, and a Western blot analysis.Cultures growing in the presence of FGF-2 showed an increased value for 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay and a decreased value for alkaline phosphatase activity. thiazolyl blue 182-242 fibroblast growth factor 2 Mus musculus 146-151 20111888-4 2011 LYP effectively inhibited ES-2 cell growth as estimated by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay and the trypan blue dye-exclusion test. thiazolyl blue 63-123 protein tyrosine phosphatase non-receptor type 22 Homo sapiens 0-3 21122942-6 2011 We also showed that treating MDCK cells with ERK1/2 inhibitor PD98059 or AKT inhibitor LY294002 diminished cytoprotection against cell death by glycine, as determined by assessment of lactate dehydrogenase release and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide activity. thiazolyl blue 218-278 mitogen-activated protein kinase 1 Canis lupus familiaris 45-51 21572341-4 2011 Knockdown of GLI2 in 3 MB cell lines resulted in decreased cell number and viability, as determined by the MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay; knockdown of GLI1 had no effect. thiazolyl blue 112-172 GLI family zinc finger 2 Homo sapiens 13-17 21289293-11 2011 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to study the function of S100A4 induction in colon cancer cells under the condition of hyperosmotic stress; the results suggest that S100A4 induction contributes to cell survival. thiazolyl blue 4-64 S100 calcium binding protein A4 Homo sapiens 219-225 21671657-6 2011 Lactate dehydrogenase and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays revealed that the Rev peptide-AuNP nanocomposites exhibited exceptionally high cytotoxic effects toward mouse ovarian surface epithelial cell lines, relative to the effects of equal doses of the free Rev peptide. thiazolyl blue 26-86 REV3 like, DNA directed polymerase zeta catalytic subunit Mus musculus 112-115 21633597-5 2011 IL-6 induced endothelial cell proliferation was measured with 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazoliumbromide assay, in vitro angiogenesis was determined with endothelial cell tube formation assay in Matrigel, and IL-6-induced angiogenesis in vitro was measured with Matrigel plug assay. thiazolyl blue 62-124 interleukin 6 Homo sapiens 0-4 21438722-4 2011 METHODS: We used reverse transcriptase polymerase chain reaction (PCR) to assay expression of GCSF and GCSF receptor (GCSFR) genes; immunostaining and flow cytometry to assay the presence of GCSFR on cell surfaces; bromodeoxyuridine (BrdU) incorporation measurement to monitor DNA synthesis; and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay to monitor cell proliferation. thiazolyl blue 296-356 colony stimulating factor 3 receptor Homo sapiens 191-196 21539450-3 2011 The effect of UHRF1-shRNA on breast cancer cell proliferation was examined using methylthiazoletetrazolium, bromodeoxyuridine, and colony formation assays. thiazolyl blue 81-106 ubiquitin like with PHD and ring finger domains 1 Homo sapiens 14-19 21542687-9 2011 IC(50) values, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium assay, showed superior antiproliferative activity of transferrin-conjugated NPs over unconjugated NPs and native nutlin-3a, owing to enhanced cellular uptake by cancer cells. thiazolyl blue 32-85 transferrin Homo sapiens 139-150 21126853-4 2011 After assessing knockdown efficiency and specificity by real-time polymerase chain reaction and Western blot, the effect of FHL1 knockdown on cell proliferation and apoptosis was evaluated by 3- (4,5-Dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide assay and flow cytometry, respectively. thiazolyl blue 192-254 four and a half LIM domains 1 Rattus norvegicus 124-128 21142943-1 2011 In this study, the protective effects of 17 Korean native plants against amyloid beta peptide (Abeta)-induced oxidative stress were screened using the 2",7"-dichlorofluorescin diacetate assay and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 200-260 amyloid beta (A4) precursor protein Mus musculus 95-100 20812778-7 2011 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) values indicated higher cell viability in samples cultured with HGF compared to CGF (P = 0.024). thiazolyl blue 0-61 hepatocyte growth factor Mus musculus 132-135 20862766-4 2010 Cellular succinate dehydrogenase (SDH) activity was estimated using the MTT method (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole), and activities were normalized to Teflon controls. thiazolyl blue 84-144 aminoadipate-semialdehyde synthase Mus musculus 9-32 20619442-7 2010 To examine the function of ADORA2B, we applied an ADORA2B-selective antagonist (MRS1754) to the colon carcinoma cells, which significantly inhibited cell growth in a dose-dependent manner as demonstrated with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell proliferation assay. thiazolyl blue 211-271 adenosine A2b receptor Homo sapiens 50-57 21467633-4 2011 The cellular response to TGF-beta1 by hPDL cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Western blot analysis. thiazolyl blue 72-132 transforming growth factor beta 1 Homo sapiens 25-34 21467633-4 2011 The cellular response to TGF-beta1 by hPDL cells was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Western blot analysis. thiazolyl blue 72-132 programmed cell death 1 Homo sapiens 38-42 21169258-6 2010 In addition, the AFP expression- and hypoxia-dependent in vitro cytotoxicity of Ad-HRE(6)/hAFPDelta19 and Ad-HRE(12)/hAFPDelta19 was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cytopathic effect assay. thiazolyl blue 147-207 alpha fetoprotein Homo sapiens 17-20 20862766-4 2010 Cellular succinate dehydrogenase (SDH) activity was estimated using the MTT method (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole), and activities were normalized to Teflon controls. thiazolyl blue 84-144 aminoadipate-semialdehyde synthase Mus musculus 34-37 20564047-7 2010 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays demonstrated that oligomeric PrP induced significant damage in and apoptosis of neuroblastoma SK-N-SH cells. thiazolyl blue 0-60 prion protein Homo sapiens 122-125 20878108-5 2010 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) analysis demonstrated that treatment with different concentrations of human UII (10(-9), 10(-8), 10(-7) and 10(-6) M) for 48 h significantly increased the number of A549 cells. thiazolyl blue 0-61 urotensin 2 Homo sapiens 144-147 20649598-3 2010 EXPERIMENTAL APPROACH: Cytotoxicity of CA-4 was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, propidium iodide (PI) staining assay and clonogenic survival assay. thiazolyl blue 60-120 carbonic anhydrase 4 Mus musculus 39-43 20152939-6 2010 Studies with a non-binding substrate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) suggest changes in activity toward cytochrome c and CYP2B1 reflect alterations in the route of electron transfer caused by the mutations. thiazolyl blue 38-98 cytochrome c, somatic Homo sapiens 140-152 21054985-6 2010 The chemosensitivity of BGC823-S100P cell line to oxaliplatin was detected by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. thiazolyl blue 78-130 S100 calcium binding protein P Homo sapiens 31-36 20693714-4 2010 The expression of HDAC7 in cell lines was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry, the nude mice modle, and vascular endothelial cells 2-dimensional tubulogenesis in vitro and in vivo. thiazolyl blue 56-116 histone deacetylase 7 Mus musculus 18-23 19286191-6 2010 The in vitro proliferative ability of CD147/EMMPRIN-deficient cells was determined by a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. thiazolyl blue 88-148 basigin (Ok blood group) Homo sapiens 38-43 19286191-6 2010 The in vitro proliferative ability of CD147/EMMPRIN-deficient cells was determined by a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. thiazolyl blue 88-148 basigin (Ok blood group) Homo sapiens 44-51 20333786-4 2010 The effect of PDGFRL protein on CRC HCT-116 cells was detected by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT), clone counting, cell cycle, and wound healing assay. thiazolyl blue 66-125 platelet derived growth factor receptor like Homo sapiens 14-20 20397263-4 2010 The relation between the responses of cell proliferation and migration to S1P and S1P5 expression was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and migration assay, respectively. thiazolyl blue 115-176 sphingosine-1-phosphate receptor 5 Homo sapiens 82-86 19909012-3 2009 Analysis with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry (FCM) demonstrated that overexpression of hARD1 was associated with increased proliferation of MCF-7 cell, a human breast cancer cell line. thiazolyl blue 14-74 N-alpha-acetyltransferase 10, NatA catalytic subunit Homo sapiens 142-147 19914370-2 2010 METHODS: In this study, we determined the effect of TGP on the proliferation of human vascular endothelial cells through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and fluorescence-activated cell sorting analysis. thiazolyl blue 121-181 transglutaminase 4 Homo sapiens 52-55 20136436-3 2010 The data showed that the antiproliferative effect of TSL-1 on H441 cells is prominent using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 96-156 TSL Homo sapiens 53-56 20211059-8 2010 NT4-p53(N15)-Ant on HepG2 cells was measured by a colorimetric 3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 63-124 neurotrophin 4 Homo sapiens 0-3 20211059-8 2010 NT4-p53(N15)-Ant on HepG2 cells was measured by a colorimetric 3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 63-124 tumor protein p53 Homo sapiens 4-7 20211059-8 2010 NT4-p53(N15)-Ant on HepG2 cells was measured by a colorimetric 3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 63-124 solute carrier family 25 member 6 Homo sapiens 13-16 19728025-5 2009 The growth inhibition of SP2/0 cell proliferation induced by artesunate (ART) treatment was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method and the rate of apoptosis and cell cycle changes induced by ART were analyzed by flow cytometry. thiazolyl blue 107-167 Sp2 transcription factor Mus musculus 25-28 19631377-5 2009 IC(50) doses as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay showed the superior antiproliferative activity of EGFR-Rapa-NPs over unconjugated nanoparticles and native rapamycin due to higher cellular uptake on malignant breast cancer cells. thiazolyl blue 30-83 epidermal growth factor receptor Homo sapiens 146-150 19631377-5 2009 IC(50) doses as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay showed the superior antiproliferative activity of EGFR-Rapa-NPs over unconjugated nanoparticles and native rapamycin due to higher cellular uptake on malignant breast cancer cells. thiazolyl blue 30-83 transcriptional regulating factor 1 Homo sapiens 151-155 19546835-5 2009 RESULTS: A 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and fluorescence activated cell scanning (FACS) revealed that REG4-overexpressing cells were resistant to gamma-radiation but showed a modest resistance to gemcitabine. thiazolyl blue 11-73 regenerating family member 4 Homo sapiens 148-152 19593667-8 2009 It also observed that PXR, activated by rifampicin or knocked down via short hairpin RNAs, could enhance or reduce cells resistance to the chemotherapeutic agents through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 171-231 nuclear receptor subfamily 1 group I member 2 Homo sapiens 22-25 19487294-7 2009 Additionally, down-regulation of vHNF1 expression in OV207 and TOV-21G cells increased cisplatin- or paclitaxel-mediated cytotoxicity as determined by both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and clonogenic assays and this effect was reversed by down-regulation of HSulf-1. thiazolyl blue 156-216 HNF1 homeobox B Homo sapiens 33-38 19491398-9 2009 MTT (3-(4,5-di meth yl thi azol-2-yl)-2,5-diphenyltetrazolium bromide) assays and fluorescent-activated cell sorter analysis revealed that ZBTB5 stimulated both cell proliferation and cell cycle progression, significantly increasing the number of cells in S-phase. thiazolyl blue 5-69 zinc finger and BTB domain containing 5 Homo sapiens 139-144 19347411-5 2009 When cell proliferation was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, we noticed a small but significant increase in growth rates with PTEN reduction. thiazolyl blue 45-105 phosphatase and tensin homolog Homo sapiens 179-183 19483313-6 2009 Isoproterenol attenuated Ang II-induced cell proliferation, as evidenced by the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay. thiazolyl blue 80-141 angiotensinogen Rattus norvegicus 25-31 19548270-9 2009 Furthermore, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed that SPAG8 inhibited the proliferation of the stable cells. thiazolyl blue 13-73 sperm associated antigen 8 Homo sapiens 98-103 19192169-4 2009 METHODS: We detected the effect of conditioned medium (CM) from human HCC cells on the activation of human HSC line, LX-2 by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and reverse transcriptase-polymerase chain reaction (RT-PCR). thiazolyl blue 127-187 fucosyltransferase 1 (H blood group) Homo sapiens 107-110 19270721-3 2009 Enhancement of cancer cell death using a combination of ZD55-IL-24 and cisplatin was assessed in several cancer cell lines by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cytopathic effect (CPE) assay. thiazolyl blue 130-190 interleukin 24 Homo sapiens 61-66 19326568-5 2009 Moreover, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and [3H] thymidine incorporation assay, it was found that exposure to CTN-986 for 4 days dose-dependently increased the proliferation of the cultured hippocampal neural progenitor cells (NPCs). thiazolyl blue 16-77 calcitonin/calcitonin-related polypeptide, alpha Mus musculus 160-163 19257909-7 2009 The effects of Id2 and its mutants on cell proliferation were determined by [3H]-thymidine incorporation assay and the 3- [4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) dye method. thiazolyl blue 119-182 inhibitor of DNA binding 2 Homo sapiens 15-18 19243318-4 2009 METHODS: Using cultured rat dopaminergic neuroblasts (N27 line), tPA-induced cytotoxicity was quantitated by an MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and flow cytometry using propidium iodide DNA staining. thiazolyl blue 117-177 plasminogen activator, tissue type Rattus norvegicus 65-68 18971960-8 2009 MiTF-negative melanoma cells survived more poorly under ROS stress than the MiTF-positive cells based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Trypan blue staining. thiazolyl blue 105-165 melanocyte inducing transcription factor Homo sapiens 0-4 18818047-3 2009 MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay showed that QA1 and QA3 weakly inhibited the growth of tumor cells. thiazolyl blue 5-66 major histocompatibility complex, class I, E Homo sapiens 86-89 19124461-10 2009 Cellular proliferation of Panc-1 cells was inhibited by INSM1 overexpression demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, soft agar colony formation, as well as tumor growth in a nude mouse model. thiazolyl blue 93-153 INSM transcriptional repressor 1 Homo sapiens 56-61 19276370-5 2009 Knockdown of Twist1 gene expression in the rat choroid plexus epithelial cell line Z310 significantly reduced proliferation as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and cell invasion in a Matrigel assay, whereas cell migration was not affected. thiazolyl blue 139-199 twist family bHLH transcription factor 1 Rattus norvegicus 13-19 18971960-8 2009 MiTF-negative melanoma cells survived more poorly under ROS stress than the MiTF-positive cells based on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Trypan blue staining. thiazolyl blue 105-165 melanocyte inducing transcription factor Homo sapiens 76-80 19026164-4 2008 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and trypan blue assays found that the cells transfected with a nm23- H1-expressing plasmid had higher viability and stronger resistance to oxidative stress. thiazolyl blue 0-60 NME/NM23 nucleoside diphosphate kinase 1 Homo sapiens 124-128 19076793-7 2009 We chose Candida albicans, Aspergillus fumigatus and Trichophyton rubrum strains as experimental targets to evaluate the inhibition of hyphal growth activity of AMCase by the disc diffusion susceptibility test, macrodilution method and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. thiazolyl blue 241-301 chitinase acidic Homo sapiens 161-167 18978578-7 2008 The proliferating activity of rat NP cells stimulated with leptin was also evaluated by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and by immunostaining for proliferation cell nuclear antigen (PCNA). thiazolyl blue 94-154 leptin Rattus norvegicus 59-65 18949367-6 2008 Using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, western blot, Hoechst 33258 staining assay and flow cytometry, we found that over-expression of UCH-L1 in MCF7 cells induced apoptosis. thiazolyl blue 6-67 ubiquitin C-terminal hydrolase L1 Homo sapiens 177-183 18941464-3 2008 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and crystal violet assays demonstrated that Cyr61 was essential for the proliferation of PCa cells. thiazolyl blue 0-60 cellular communication network factor 1 Homo sapiens 111-116 18367439-8 2008 Conversely, NSC-34 cell smn overexpression by adenoviral gene transfer decreased staurosporine-induced caspase-3 elevation and mitigated induced cell toxicity as assessed by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. thiazolyl blue 174-236 survival motor neuron 1 Mus musculus 24-27 18800168-1 2008 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) reduction assay is a frequently used and easily reproducible method to measure beta-amyloid (Abeta) toxicity in different types of single cell culture. thiazolyl blue 4-64 amyloid beta precursor protein Rattus norvegicus 164-169 18021219-6 2008 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays showed that cell growth inhibition directly correlated to the level of activated c-Met detected in each cell line (r =-0.87, P = 0.012). thiazolyl blue 0-60 MET proto-oncogene, receptor tyrosine kinase Homo sapiens 155-160 18404163-4 2008 When transfected into the prostate cancer cell line PC3, DZ2 was found to strongly inhibit the expression of Aurora kinase A examined by western blot analysis, and thus suppressed cell growth, arrested the progression of cell cycle, induced cell apoptosis and attenuated cell migration, as measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay, flow cytometry and Boyden chamber assay. thiazolyl blue 302-354 aurora kinase A Homo sapiens 109-124 18636193-5 2008 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay indicated that overexpression of S100P sensitized OVCAR3 cells for chemotherapeutic drugs (paclitaxel, oxaliplatin, 5-fluorouracil, etoposide and epirubicin) induced cytotoxicity more than vector-only controls. thiazolyl blue 0-52 S100 calcium binding protein P Homo sapiens 98-103 18465096-6 2008 Time-dependent effects of varying doses of EPO (0.1 to 50 IU/ml) treatment during lipopolysaccharide (LPS)-mediated cytotoxicity was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide test. thiazolyl blue 145-205 erythropoietin Homo sapiens 43-46 18187583-6 2008 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assays demonstrated that EGFR and k-Ras mutations were related to erlotinib sensitivity, whereas TS and DHFR expression were related to pemetrexed sensitivity. thiazolyl blue 0-52 epidermal growth factor receptor Homo sapiens 78-82 18187583-6 2008 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assays demonstrated that EGFR and k-Ras mutations were related to erlotinib sensitivity, whereas TS and DHFR expression were related to pemetrexed sensitivity. thiazolyl blue 0-52 KRAS proto-oncogene, GTPase Homo sapiens 87-92 18175960-3 2008 Gallic acid (0.1, 1 microM) showed a concentration-dependent inhibition on Abeta (25-35) (10 microM)-induced apoptotic neuronal death, as assessed by a 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33,342 staining. thiazolyl blue 152-214 amyloid beta precursor protein Rattus norvegicus 75-80 18175932-4 2008 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) and flow cytometry assays demonstrated that leptin significantly promoted myoblast proliferation and increased cell accumulation in the S + G2/M phase, in a dose-dependent manner. thiazolyl blue 0-61 leptin Homo sapiens 112-118 17932440-6 2007 3-(4,5- dimethylthiazole-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay was to investigate the effects of PAF on ASMC proliferation and to confirm its optimum concentration for action. thiazolyl blue 0-62 PCNA clamp associated factor Rattus norvegicus 109-112 17958740-4 2007 METHODS: The effect of thrombin on cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. thiazolyl blue 75-136 coagulation factor II, thrombin Homo sapiens 23-31 17804744-5 2007 Using multiple cellular and molecular approaches such as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, gene transfection, flow cytometry, real-time reverse transcription-PCR, Western blotting, migration, invasion, and angiogenesis assays, we found that down-regulation of FoxM1 inhibited cell growth, decreased cell migration, and decreased invasion of pancreatic cancer cells. thiazolyl blue 57-117 forkhead box M1 Homo sapiens 294-299 17635674-4 2007 Exposure of LC neurons to recombinant-TNF-alpha resulted in decreased metabolic activity, an indicator of reduced neuron viability [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay], and increased apoptosis (terminal deoxynucleotidyl transferase-mediated DNA nick end labeling assay). thiazolyl blue 132-192 tumor necrosis factor Rattus norvegicus 38-47 17764703-1 2007 When cardiomyocytes were subjected to hypoxia, tumor necrosis factor-alpha (TNF-alpha; 3-50 ng/ml) or adenosine (1-100 microM), decreased hypoxic damage as was detected by lactate dehydrogenase (LDH) release, MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) absorbance, ROS (reactive oxygen species) measurement or desmin immunostaining. thiazolyl blue 214-274 tumor necrosis factor Homo sapiens 47-74 17764703-1 2007 When cardiomyocytes were subjected to hypoxia, tumor necrosis factor-alpha (TNF-alpha; 3-50 ng/ml) or adenosine (1-100 microM), decreased hypoxic damage as was detected by lactate dehydrogenase (LDH) release, MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) absorbance, ROS (reactive oxygen species) measurement or desmin immunostaining. thiazolyl blue 214-274 tumor necrosis factor Homo sapiens 76-85 17383127-2 2007 In this study, the effect of KIOM-79 on HG or S100b-induced VEGF expression was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, RT-PCR, ELISA, and Western blot on human RPE cells. thiazolyl blue 99-160 S100 calcium binding protein B Homo sapiens 46-51 17441962-3 2007 Using quantitative real-time polymerase chain reaction and Western blot, we found that overexpression of CD147 in MCF7 cells up-regulated MDR1, MMP2, and MMP9 on both transcription and expression levels, which promoted tumor cells metastasis and conferred them multidrug resistance to P-gp substrate drugs, as determined by in vitro invasion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 352-412 basigin (Ok blood group) Homo sapiens 105-110 17441962-3 2007 Using quantitative real-time polymerase chain reaction and Western blot, we found that overexpression of CD147 in MCF7 cells up-regulated MDR1, MMP2, and MMP9 on both transcription and expression levels, which promoted tumor cells metastasis and conferred them multidrug resistance to P-gp substrate drugs, as determined by in vitro invasion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 352-412 ATP binding cassette subfamily B member 1 Homo sapiens 138-142 17511985-4 2007 The present study was designed to investigate the effect of 17beta-estradiol on the angiotensin II-induced proliferation and collagen synthesis in cultured cardiac fibroblasts by using real-time polymerase chain reaction (PCR), Western blot and 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyl-tetrazolium bromide proliferation assay. thiazolyl blue 245-307 angiotensinogen Rattus norvegicus 84-98 17695534-3 2007 Antineoplaston A10 displayed anti-proliferative action inhibiting cell growth in a dose- and time-dependent manner in vitro as measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and clonogenic assays. thiazolyl blue 139-199 immunoglobulin kappa variable 6D-21 (non-functional) Homo sapiens 15-18 17409520-4 2007 Here, the possible effects of a recombinant buckwheat trypsin inhibitor (rBTI) on the induction of apoptosis of the human K562 cell line were investigated by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assays and flow cytometric analysis. thiazolyl blue 163-223 cysteine rich secretory protein LCCL domain containing 2 Homo sapiens 54-71 17439729-2 2007 METHODS: The effect of insulin/5-FU combination treatment on the growth of Eca 109 and Ls-174-t cells was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. thiazolyl blue 119-180 insulin Homo sapiens 23-30 17543181-6 2007 The effects and mechanisms of the cyclin L2 in cell growth, cell cycle analysis and apoptosis were studied by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), flow cytometry or Western blot, respectively. thiazolyl blue 110-171 cyclin L2 Homo sapiens 34-43 17444856-4 2007 Wild-type RASSF1A cDNA was introduced into SMMC-7721; chemosensitivity to cisplatin, mitomycin and fluorouracil were analyzed using 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay. thiazolyl blue 132-194 Ras association domain family member 1 Homo sapiens 10-17 17351395-3 2007 The effect of anti-transketolase-like-1 small interfering RNA on cell proliferation and cell cycle in the human colon cancer cell line cells was detected by flow cytometry and 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide. thiazolyl blue 176-236 transketolase Homo sapiens 19-32 17135250-6 2007 Moreover, HCT116 colon cancer cells exhibited a p53-dependent sensitivity to PpIX in a dose-dependent manner, as was demonstrated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and fluorescence-activated cell sorter (FACS) analysis of cell cycle profiles. thiazolyl blue 136-196 tumor protein p53 Homo sapiens 48-51 16912864-6 2007 METHODS: Proliferation of HT-29 colon cancer cells in response to leptin was assessed by 3-[4, 5-dimethylthiazol-2-y-l]-2, 5-diphenyltetrazolium bromide (MTT) assay, and apoptosis was quantified by enzyme-linked immunosorbent assay (ELISA) for intracellular nucleosomes. thiazolyl blue 89-152 leptin Homo sapiens 66-72 17454250-4 2007 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability decreased significantly (p < .05) from 0.00005 to 0.05 mg/ml after 24 h. The proinflammatory mediators of inflammation cytokines interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-alpha, IL-10, and IL-1beta were also assessed. thiazolyl blue 5-65 C-X-C motif chemokine ligand 8 Homo sapiens 234-238 17454250-4 2007 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability decreased significantly (p < .05) from 0.00005 to 0.05 mg/ml after 24 h. The proinflammatory mediators of inflammation cytokines interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-alpha, IL-10, and IL-1beta were also assessed. thiazolyl blue 5-65 tumor necrosis factor Homo sapiens 240-273 17454250-4 2007 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability decreased significantly (p < .05) from 0.00005 to 0.05 mg/ml after 24 h. The proinflammatory mediators of inflammation cytokines interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-alpha, IL-10, and IL-1beta were also assessed. thiazolyl blue 5-65 interleukin 10 Homo sapiens 275-280 17454250-4 2007 MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cell viability decreased significantly (p < .05) from 0.00005 to 0.05 mg/ml after 24 h. The proinflammatory mediators of inflammation cytokines interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-alpha, IL-10, and IL-1beta were also assessed. thiazolyl blue 5-65 interleukin 1 beta Homo sapiens 286-294 17157826-3 2007 The first method takes advantage of the unique ability of extracellularly applied Abeta oligomers to rapidly induce the exocytosis of formazan formed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). thiazolyl blue 153-213 amyloid beta precursor protein Homo sapiens 82-87 16978655-3 2006 Catechin and epicatechin inhibited 10 microM Abeta (25-35)-induced neuronal cell death at a concentration of 10 microM, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. thiazolyl blue 144-205 amyloid beta precursor protein Rattus norvegicus 45-50 17979707-2 2007 Clonogenic assay in soft agar and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed that many endometrial and ovarian cancer cell lines were sensitive to the growth inhibitory effect of HDAC inhibitors (HDACIs), although normal endometrial epithelial cells were viable after the treatment with the same doses of HDACIs that induced growth inhibition of endometrial and ovarian cancer cells. thiazolyl blue 34-94 histone deacetylase 9 Homo sapiens 211-215 17159501-4 2007 The results showed that GC-1 inhibited growth of a panel of human cancer cell lines in vitro by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide and sulforhodamine B assays. thiazolyl blue 96-156 olfactomedin 4 Homo sapiens 24-28 16769811-3 2006 We determined the presence and functionality of BMPR1A by examining BMP-induced phosphorylation and nuclear translocation of SMAD1; transcriptional activity via a BMP-specific luciferase reporter; and growth characteristics by cell cycle analysis, cell growth, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide metabolic assays. thiazolyl blue 265-325 bone morphogenetic protein receptor type 1A Homo sapiens 48-54 17063121-7 2006 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tests demonstrated that at doses ranging from 0.2 to 2 microg/mL, this fusion protein specifically killed CD28-overexpressing Jurkat cells but even at doses of 2 microg did not kill CD28-negative Hut28 cells. thiazolyl blue 0-60 CD28 molecule Homo sapiens 167-171 16895977-1 2006 In the mouse leukemic monocyte cell line RAW 264.7, the vacuolar-type (H(+))-ATPase (V-ATPase) inhibitors bafilomycin A1 and concanamycin A induced nitric oxide (NO) production through the expression of inducible nitric-oxide synthase mRNA and its protein and decreased cell growth and survival as determined by 3-(4,5-dimethyl(thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 312-373 ATPase, H+ transporting, lysosomal V0 subunit D2 Mus musculus 85-93 16885370-8 2006 The results revealed that Med1-null MEFs are more sensitive to 5IdU than wild-type MEFs in both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays. thiazolyl blue 96-156 mediator complex subunit 1 Mus musculus 26-30 16765919-6 2006 Interestingly, overexpression of TRPC1 increases TRPC1 protein levels and also protected SH-SY5Y neuroblastoma cells against salsolinol-mediated cytotoxicity as determined by 3,[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 175-235 transient receptor potential cation channel subfamily C member 1 Homo sapiens 33-38 16769811-3 2006 We determined the presence and functionality of BMPR1A by examining BMP-induced phosphorylation and nuclear translocation of SMAD1; transcriptional activity via a BMP-specific luciferase reporter; and growth characteristics by cell cycle analysis, cell growth, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide metabolic assays. thiazolyl blue 265-325 bone morphogenetic protein 1 Homo sapiens 48-51 16316652-4 2006 Both the 3-(4,5-dimethylthiazol-2yl-)-2,5-diphenyl tetrazolium bromide and trypan blue staining assays also showed the quercetin-mediated facilitation of TNF-alpha-induced cytotoxicity in the cells. thiazolyl blue 9-70 tumor necrosis factor Mus musculus 154-163 16497458-4 2006 SCR, over a concentration range of 10-50 microg/ml, inhibited 10 microM Abeta (25-35)-induced neuronal cell death, which was measured by a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. thiazolyl blue 139-200 amyloid beta precursor protein Rattus norvegicus 72-77 16497702-6 2006 Indeed, overexpression of p65 enhanced curcumin-mediated apoptosis as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) assay and poly(ADP-ribose) polymerase (PARP) cleavage. thiazolyl blue 82-141 RELA proto-oncogene, NF-kB subunit Homo sapiens 26-29 16630105-8 2006 In vitro, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide results showed that c-ski could reverse the inhibitory role of Smad3 on fibroblast proliferation. thiazolyl blue 10-70 SMAD family member 3 Homo sapiens 134-139 16403434-6 2006 The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay demonstrates that f-adiponectin inhibits prostate cancer cell growth drastically at subphysiological concentrations. thiazolyl blue 4-65 adiponectin, C1Q and collagen domain containing Homo sapiens 92-103 16292251-5 2006 Astrocytes overexpressing Hsp70-WT, -K71E, or -381-640 were all significantly protected from 4 h combined oxygen-glucose deprivation and 24 h reperfusion when assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay or propidium iodide staining and cell counting (P < 0.05). thiazolyl blue 171-231 heat shock protein family A (Hsp70) member 1B Rattus norvegicus 26-31 16409727-5 2005 The effect of BDNF on the in vitro proliferation of HMCLs (RPMI 8226, U266, KM3) was examined with 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. thiazolyl blue 99-161 brain derived neurotrophic factor Homo sapiens 14-18 17073723-8 2006 Using the method of methylthiazoletetrazolium, the mitogenic activity on Balb/c 3T3 cells of the purified hEGF was comparable to that of commercial hEGF. thiazolyl blue 20-45 epidermal growth factor Homo sapiens 106-110 16626551-7 2006 The A(490nm) values obtained by MTT measurements were similar among groups in the absence of Ang II but decreased significantly in pAT1a-shRNA1 and pAT1a-shRNA2 treated VSMCs in the presence of Ang II. thiazolyl blue 32-35 angiotensinogen Rattus norvegicus 194-200 16271076-5 2005 In vitro cytotoxic effects of Lep were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-based viability assay. thiazolyl blue 54-114 leptin Homo sapiens 30-33 16140321-7 2005 3-[4, 5-Dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay showed that basal level of mitochondrial function was lower in MnSOD(+/-) cardiomyocytes than in NTg or TgH, and that MnSOD(+/-) was more sensitive to ADR. thiazolyl blue 0-62 superoxide dismutase 2, mitochondrial Mus musculus 138-143 16211296-7 2005 The RQV of MGMT in these cells treated with platinum compounds obviously decreased, and these cells were more sensitive to ACNU than the control cells based on colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 173-233 O-6-methylguanine-DNA methyltransferase Homo sapiens 11-15 16227413-4 2005 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay indicated that YC-1 suppresses growth of PC-3 cells in a concentration-dependent and time-dependent manner. thiazolyl blue 0-61 RNA binding motif single stranded interacting protein 1 Homo sapiens 83-87 15930289-4 2005 In addition, forced overexpression of CYP2J2, and CYP BM3F87V or addition of epoxyeicosatrienoic acids (EET) in cultured carcinoma cell lines in vitro markedly accelerated proliferation by analyses of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cell accounts, and cell cycle analysis, and protected carcinoma cells from apoptosis induced by tumor necrosis factor alpha (TNF-alpha) in cultures. thiazolyl blue 201-261 cytochrome P450 family 2 subfamily J member 2 Homo sapiens 38-44 16115953-7 2005 Moreover, cyclin D1-overexpressing cells exhibited significantly reduced chemosensitivity and a higher survival rate upon cisplatin treatment, as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and clonogenic assays, respectively. thiazolyl blue 160-220 cyclin D1 Homo sapiens 10-19 15911748-4 2005 MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay with A549 cells showed that Cyp-GRD was not cytotoxic up to 100 muM in cell culture. thiazolyl blue 5-65 peptidylprolyl isomerase G Homo sapiens 101-104 16045007-5 2005 The effect of suppressing beta-catenin by RNAi on cell proliferation was quantified by methylthiazoletetrazolium (MTT) assay. thiazolyl blue 87-112 catenin beta 1 Homo sapiens 26-38 16045007-5 2005 The effect of suppressing beta-catenin by RNAi on cell proliferation was quantified by methylthiazoletetrazolium (MTT) assay. thiazolyl blue 114-117 catenin beta 1 Homo sapiens 26-38 16045007-8 2005 MTT showed that the cell number of beta-catenin expression suppression cell clones decreased significantly (P < 0. thiazolyl blue 0-3 catenin beta 1 Homo sapiens 35-47 15943915-0 2005 A continuous spectrophotometric assay for NADPH-cytochrome P450 reductase activity using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. thiazolyl blue 89-149 cytochrome p450 oxidoreductase Homo sapiens 42-73 15883728-2 2005 Cytotoxicity assay using 3(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) was carried out with deoxynivalenol (DON), T-2 toxins and zearalenone (ZON) on CaCo-2, HEp-2 and HeLa cell lines. thiazolyl blue 25-83 solute carrier family 25 member 5 Homo sapiens 133-136 15943915-3 2005 In this report, reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by CPR has been assessed as a method for monitoring CPR activity. thiazolyl blue 29-89 cytochrome p450 oxidoreductase Homo sapiens 99-102 15943915-3 2005 In this report, reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) by CPR has been assessed as a method for monitoring CPR activity. thiazolyl blue 29-89 cytochrome p450 oxidoreductase Homo sapiens 148-151 15837750-3 2005 EXPERIMENTAL DESIGN: Long-term clinical outcome in 45 t(12;21)+ ALL patients was related to mRNA expression of TEL, AML1, TEL-AML1, and AML1-TEL, determined by real-time quantitative PCR, and the in vitro sensitivity to prednisolone, vincristine, and L-asparaginase, using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. thiazolyl blue 273-333 ETS variant transcription factor 6 Homo sapiens 111-114 15837745-9 2005 The SDF-1-mediated cell proliferation was analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 54-114 C-X-C motif chemokine ligand 12 Homo sapiens 4-9 15448167-11 2004 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays and cell cycle analysis revealed that, in addition to having an additive effect on ERK1/2 activation, cotreatment with IGF-1 and LHRH also had an additive effect on cell proliferation. thiazolyl blue 0-60 insulin like growth factor 1 Homo sapiens 187-192 15960268-5 2005 The cytotoxity of MDR1-AS was tested using morphological observation and 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl tetrazolium bromide assay. thiazolyl blue 73-137 ATP binding cassette subfamily B member 1 Homo sapiens 18-22 29662387-5 2005 Methods: The concentrations and biological activity of soluble TNF-alpha protein present in oviductal fluid (OF) and uterine fluid (UF), in the normal and LPS treated animals, were determined by enzyme-linked immunosorbent assay and 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyltetrazolium bromide assay on L929 cells, respectively. thiazolyl blue 234-294 tumor necrosis factor Mus musculus 64-73 15688034-6 2005 The HGF/SF-mediated protection of DU-145 and MDCK cells against ADR (demonstrated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays) was abrogated by the IkappaB-alpha super-repressor. thiazolyl blue 93-153 hepatocyte growth factor Homo sapiens 4-10 15598681-5 2005 The [(3)H]thymidine incorporation and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays revealed that treatment with GnRH-II suppresses cell proliferation of ovarian cancer cells. thiazolyl blue 38-98 gonadotropin releasing hormone 2 Homo sapiens 141-148 15448167-11 2004 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays and cell cycle analysis revealed that, in addition to having an additive effect on ERK1/2 activation, cotreatment with IGF-1 and LHRH also had an additive effect on cell proliferation. thiazolyl blue 0-60 gonadotropin releasing hormone 1 Homo sapiens 197-201 15585621-2 2004 EXPERIMENTAL DESIGN: Susceptibility of HCC cell lines to TRAIL and/or 5-FU was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 91-151 TNF superfamily member 10 Homo sapiens 57-62 15269152-4 2004 Growth-inhibitory effects of decorin in vitro were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test, Western blot, and fluorescence-activated cell-sorting analysis. thiazolyl blue 63-123 decorin Homo sapiens 29-36 15192088-4 2004 In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays, HeLa cells treated with 5 ng/ml of TGF-beta for 24 h showed proliferation, whereas SiHa cells showed growth inhibition under the same conditions. thiazolyl blue 3-63 transforming growth factor beta 1 Homo sapiens 107-115 15256434-4 2004 Compared with control cells, metallothionein 1X (MT1X)-transfected AGS cells showed a 1.4-fold higher irinotecan IC(50) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and tended to form more colonies. thiazolyl blue 123-183 metallothionein 1X Homo sapiens 29-47 15217495-3 2004 METHODS: KPL-1 cell growth was assessed by colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; cell cycle progression and mode of cell death were examined by flow cytometry; and levels of expression of p53, p21Cip1/Waf1, cyclin D1, Bax, and Bcl-2 proteins were examined by Western blotting analysis. thiazolyl blue 56-116 pleckstrin homology domain containing B1 Homo sapiens 9-14 15256434-4 2004 Compared with control cells, metallothionein 1X (MT1X)-transfected AGS cells showed a 1.4-fold higher irinotecan IC(50) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and tended to form more colonies. thiazolyl blue 123-183 metallothionein 1X Homo sapiens 49-53 15252144-7 2004 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays of siRNA-treated cells demonstrated 7- to 12.4-fold reduction of paclitaxel resistance in the lines treated with the synthesized siRNA of ABCB1 and 4.7- to 7.3-fold reduction of paclitaxel resistance in the cell lines transfected with siRNA of ABCB1 expressing vectors. thiazolyl blue 0-60 ATP binding cassette subfamily B member 1 Homo sapiens 206-211 15252144-7 2004 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays of siRNA-treated cells demonstrated 7- to 12.4-fold reduction of paclitaxel resistance in the lines treated with the synthesized siRNA of ABCB1 and 4.7- to 7.3-fold reduction of paclitaxel resistance in the cell lines transfected with siRNA of ABCB1 expressing vectors. thiazolyl blue 0-60 ATP binding cassette subfamily B member 1 Homo sapiens 312-317 15126879-2 2004 MATERIALS AND METHODS: The cytotoxic effect of combined treatment with AS clusterin ODN and gemcitabine on in vitro KoTCC-1 growth was examined using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. thiazolyl blue 159-219 clusterin Homo sapiens 74-83 15135888-4 2004 Treatment with IL-1alpha or IL-6 caused a dose-dependent increase in survival as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. thiazolyl blue 97-158 interleukin 1 alpha Homo sapiens 15-24 15135888-4 2004 Treatment with IL-1alpha or IL-6 caused a dose-dependent increase in survival as measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. thiazolyl blue 97-158 interleukin 6 Homo sapiens 28-32 14966912-9 2004 The transfection of antisense VEGF(165) gene resulted in the inhibitory effect on the proliferation of hepatocarcinoma cells by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and the death of all hepatocarcinoma cells on day 6 after transfection. thiazolyl blue 128-190 vascular endothelial growth factor A Homo sapiens 30-34 15033710-2 2003 3-Nitropropionic acid (3-NP) mediated a dose-dependent decrease in cell viability in GT1-7puro cells, as determined by following the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction, which was significantly prevented in GT1-7bcl-2 cells. thiazolyl blue 138-198 retinoic acid induced 1 Mus musculus 85-88 15033710-2 2003 3-Nitropropionic acid (3-NP) mediated a dose-dependent decrease in cell viability in GT1-7puro cells, as determined by following the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction, which was significantly prevented in GT1-7bcl-2 cells. thiazolyl blue 138-198 retinoic acid induced 1 Mus musculus 248-251 12960112-4 2003 RESULTS: Ad.mda-7 caused a dose-dependent reduction in the proliferation of glioma cells in 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. thiazolyl blue 92-152 interleukin 24 Rattus norvegicus 12-17 14614897-4 2003 ApoE4, but not apoE3, also significantly increased neurotoxicity, as evidenced by enhanced lactate dehydrogenase release (by 53%) and reduced 3-(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide levels (by 32%). thiazolyl blue 142-202 apolipoprotein E Homo sapiens 0-5 12899799-6 2003 Thiazolyl blue (MTT) method was used to detect the growth of CD8(+) T cell. thiazolyl blue 0-14 CD8a molecule Homo sapiens 61-64 12594242-5 2003 In addition, NOS3 overexpression impaired neuronal Mt function as demonstrated by the reduced levels of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide and nicotinamide adenine dinucleotide (reduced form)-tetrazolium reductase activities and MitoTracker Red fluorescence. thiazolyl blue 104-164 nitric oxide synthase 3 Homo sapiens 13-17 14686785-3 2003 Overexpression of CYP2D6 protected both actively dividing and differentiated cells against the toxic effects of 1-methyl-4-phenylpyridinium ion at the concentration range of 20-40 microM, as assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 207-267 cytochrome P450, family 2, subfamily d, polypeptide 4 Rattus norvegicus 18-24 12836169-4 2003 P/M supply to control and A beta-treated neuronal cultures increases cellular reducing power, as indicated by the ability to reduce the dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). thiazolyl blue 140-200 amyloid beta precursor protein Rattus norvegicus 26-32 12855658-9 2003 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxicity analysis of both MAGE2 and MAGE6 transfectants demonstrates a 4-fold increase in resistance to paclitaxel and 2-fold increase in resistance to doxorubicin but not to other drugs, such as topotecan and cisplatin, through a nonmultidrug resistance-1 mechanism. thiazolyl blue 0-60 MAGE family member A2B Homo sapiens 91-96 12855658-9 2003 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxicity analysis of both MAGE2 and MAGE6 transfectants demonstrates a 4-fold increase in resistance to paclitaxel and 2-fold increase in resistance to doxorubicin but not to other drugs, such as topotecan and cisplatin, through a nonmultidrug resistance-1 mechanism. thiazolyl blue 0-60 MAGE family member A6 Homo sapiens 101-106 12725918-3 2003 Exposure of the cells to amyloid beta-protein(1-40) (Abeta(1-40)) decreased the extent of [3-(4,5)-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) reduction activity and loss of neuronal integrity, but these effects were not prevented by Ac-DEVD-CHO, a caspase-3 inhibitor. thiazolyl blue 90-144 amyloid beta precursor protein Rattus norvegicus 53-58 12657185-3 2003 METHODS: Gastrin-induced influence on overall growth was assayed in vitro by means of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium colorimetric assay for human U373 and rat C6 gliomas and for rat 9L gliosarcoma. thiazolyl blue 90-142 gastrin Homo sapiens 9-16 12649192-6 2003 Hence, we targeted ERCC1 by antisense RNA methodologies, and we show that we could sensitize a relatively sensitive A2780 cell line and also the highly resistant OVCAR10 cell line to cisplatin by expressing antisense ERCC1 RNA in them as measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. thiazolyl blue 252-312 ERCC excision repair 1, endonuclease non-catalytic subunit Homo sapiens 19-24 12649192-6 2003 Hence, we targeted ERCC1 by antisense RNA methodologies, and we show that we could sensitize a relatively sensitive A2780 cell line and also the highly resistant OVCAR10 cell line to cisplatin by expressing antisense ERCC1 RNA in them as measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. thiazolyl blue 252-312 ERCC excision repair 1, endonuclease non-catalytic subunit Homo sapiens 217-222 11696816-7 2001 RESULTS: ICAD transfected ACHN cells inhibited DNA degradation after cisplatin treatment but not control vector only transfected ACHN cells, whereas a similar degree of apoptotic cell death induced by cisplatin in ICAD and control vector only transfected ACHN cells was observed on 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Western blot analysis of poly(ADP-ribose) polymerase protein. thiazolyl blue 282-342 DNA fragmentation factor subunit alpha Homo sapiens 9-13 12393269-0 2002 Amyloid beta-peptide alters the distribution of early endosomes and inhibits phosphorylation of Akt in the presence of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). thiazolyl blue 119-179 AKT serine/threonine kinase 1 Homo sapiens 96-99 12393269-1 2002 Amyloid beta-peptide (Abeta) effectively inhibits the cellular reduction activity of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) in a variety of cultured cells. thiazolyl blue 85-145 amyloid beta precursor protein Homo sapiens 22-27 12234988-10 2002 In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays in receptor-bearing Chinese hamster ovary cells, the secretin receptor was shown to have growth-inhibitory effects. thiazolyl blue 3-63 secretin receptor Cricetulus griseus 124-141 12124431-3 2002 NAC fibrils decreased the activity of reducing 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) in cortical neurons more markedly (IC(50) = 5.6 microm) than in astrocytes (IC(50) approximately 50 microm). thiazolyl blue 47-107 synuclein alpha Homo sapiens 0-3 12065652-8 2002 In cell culture experiments, fAbeta disrupted by NDGA were less toxic than intact fAbeta, as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 109-169 FA complementation group B Homo sapiens 29-35 12011054-6 2002 The peptides effectively inhibited growth of two types of transformed cells overexpressing different erbB receptors, T6-17 and 32D, in standard MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and cell viability assays. thiazolyl blue 149-209 epidermal growth factor receptor Homo sapiens 101-105 12019172-11 2002 The growth suppression seen in HDAC5-overexpressing cells appears to be attributable partly to a reduced growth rate as revealed by cell growth assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and mainly to spontaneous apoptosis as shown by DNA fragmentation ELISA and morphological appearance. thiazolyl blue 156-216 histone deacetylase 5 Homo sapiens 31-36 11788422-2 2002 Palmitate-induced cell death, assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, was enhanced by carnitine, a cofactor needed for palmitate transport into mitochondria via CPT-1. thiazolyl blue 46-107 carnitine palmitoyltransferase 1A Gallus gallus 206-211 11348451-6 2001 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays showed significantly higher values in cultured skin substitutes incubated with insulin at incubation days 14 and 28 compared to negative control or the 10 ng per ml insulin-like growth factor I condition. thiazolyl blue 0-61 insulin Homo sapiens 154-161 11423547-5 2001 These non-fibrillar A beta aggregates displayed much weaker Congo Red birefringence, and in separate cell culture experiments, were less toxic than self beta-aggregates, as demonstrated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. thiazolyl blue 189-249 amyloid beta precursor protein Homo sapiens 20-26 11294760-4 2001 The results showed that ET-1 effectively attenuated Dox-induced acute cardiomyocyte cytotoxicity (24-h incubation with Dox) evaluated by in vitro cell toxicity assay [3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay and lactate dehydrogenase release]. thiazolyl blue 167-228 endothelin 1 Rattus norvegicus 24-28 11410727-4 2001 RESULTS: In ethanol-exposed, insulin-stimulated PNET2 central nervous system-derived human neuronal cells, reduced viability was associated with increased Mt DNA damage, reduced Mt mass (manifested by reduced Mt protein expression and MitoTracker Green fluorescent labeling), and impaired Mt function (manifested by reduced levels of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide activity, cytochrome oxidase-Complex IV, Subunit II expression, and MitoTracker Red fluorescence). thiazolyl blue 334-394 insulin Homo sapiens 29-36 11742594-3 2001 Toxicity of the A-beta (1 - 40) towards PC12 cells was measured with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT). thiazolyl blue 69-129 amyloid beta precursor protein Rattus norvegicus 16-22 11137176-1 2001 The effects of long-term exposure of a pancreatic beta cell line, INS-1, to major free fatty acids (FFA; palmitic acid, oleic acid and linoleic acid) and leptin on insulin secretion and cell viability by C,N-diphenyl-N"-4,5 dimethylthiazol 2-yl tetrazolium bromide (MTT) assay were examined. thiazolyl blue 204-264 leptin Rattus norvegicus 154-160 11279283-0 2001 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) causes Akt phosphorylation and morphological changes in intracellular organellae in cultured rat astrocytes. thiazolyl blue 0-60 AKT serine/threonine kinase 1 Rattus norvegicus 74-77 11223020-5 2001 Furthermore, only the pH 5.8 Abeta aggregates induced significant apoptotic death of PC12 cells, as indicated by a decrease in 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) reduction and an increase in phosphatidylserine externalization. thiazolyl blue 127-186 amyloid beta precursor protein Homo sapiens 29-34 10871651-4 2000 IFN-gamma and increasing doses of TNF-alpha resulted in decreasing viability of trophoblast on uncoated as well as fibronectin-coated dishes, as shown by 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assays, but for each TNF/IFN treatment condition viability on fibronectin was higher (P < 0.001). thiazolyl blue 154-216 interferon gamma Homo sapiens 0-9 11211874-3 2000 The cell proliferation activity of the recombinant FGF8 (rFGF8) was measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazoliumbromide (MTT) assay. thiazolyl blue 80-142 fibroblast growth factor 8 Rattus norvegicus 51-55 11211874-3 2000 The cell proliferation activity of the recombinant FGF8 (rFGF8) was measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazoliumbromide (MTT) assay. thiazolyl blue 80-142 fibroblast growth factor 8 Rattus norvegicus 57-62 11118037-4 2000 Transfection of DLC-1 into 5 HCC cell lines (two with DLC-1 deletion and three with intact DLC-1) showed significant growth inhibition in these two HCC cell lines with deleted DLC-1 with both 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and colony formation assays but not in three other HCC cell lines with intact DLC-1. thiazolyl blue 192-252 DLC1 Rho GTPase activating protein Homo sapiens 16-21 10909968-4 2000 A 72-h exposure of RINm5F control cells to interleukin-1beta (IL-1beta) alone or a combination of IL-1beta, tumor necrosis factor-alpha, and gamma-interferon resulted in a time- and concentration-dependent decrease of cell viability in the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity assay. thiazolyl blue 240-301 interleukin 1 beta Rattus norvegicus 43-60 10909968-4 2000 A 72-h exposure of RINm5F control cells to interleukin-1beta (IL-1beta) alone or a combination of IL-1beta, tumor necrosis factor-alpha, and gamma-interferon resulted in a time- and concentration-dependent decrease of cell viability in the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity assay. thiazolyl blue 240-301 interleukin 1 beta Rattus norvegicus 62-70 10909968-4 2000 A 72-h exposure of RINm5F control cells to interleukin-1beta (IL-1beta) alone or a combination of IL-1beta, tumor necrosis factor-alpha, and gamma-interferon resulted in a time- and concentration-dependent decrease of cell viability in the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity assay. thiazolyl blue 240-301 interleukin 1 beta Rattus norvegicus 98-106 10909968-4 2000 A 72-h exposure of RINm5F control cells to interleukin-1beta (IL-1beta) alone or a combination of IL-1beta, tumor necrosis factor-alpha, and gamma-interferon resulted in a time- and concentration-dependent decrease of cell viability in the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity assay. thiazolyl blue 240-301 tumor necrosis factor Rattus norvegicus 108-135 10871651-4 2000 IFN-gamma and increasing doses of TNF-alpha resulted in decreasing viability of trophoblast on uncoated as well as fibronectin-coated dishes, as shown by 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assays, but for each TNF/IFN treatment condition viability on fibronectin was higher (P < 0.001). thiazolyl blue 154-216 tumor necrosis factor Homo sapiens 34-43 10871651-4 2000 IFN-gamma and increasing doses of TNF-alpha resulted in decreasing viability of trophoblast on uncoated as well as fibronectin-coated dishes, as shown by 3-[4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assays, but for each TNF/IFN treatment condition viability on fibronectin was higher (P < 0.001). thiazolyl blue 154-216 fibronectin 1 Homo sapiens 115-126 10454051-5 1999 Mitracarpus treatment also resulted in a good ( > 93%) survival rate for the CCl4-intoxicated hepatocytes as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. thiazolyl blue 128-189 C-C motif chemokine ligand 4 Rattus norvegicus 80-84 10452234-7 1999 We should therefore pay close attention to the effect of P-glycoprotein when treating cancer patients, especially if both the inhibition rates of DOX and MMC are low based on the findings of an MTT assay. thiazolyl blue 194-197 ATP binding cassette subfamily B member 1 Homo sapiens 57-71 10350568-1 1999 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay has been widely used for evaluating amyloid beta protein (Abeta) toxicity. thiazolyl blue 0-60 amyloid beta precursor protein Rattus norvegicus 141-146 9832130-1 1998 Perhaps the most reproducible early event induced by the interaction of amyloid beta peptide (A beta) with the cell is the inhibition of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. thiazolyl blue 146-206 amyloid beta precursor protein Homo sapiens 94-100 9067460-4 1997 beta(25-35), an active sequence of A beta, showed dose-dependent reduction of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide dye (MTT) reductivity, and chlorpromazine (CPZ), promethazine or trifluoperazine restored it at micromolar concentration. thiazolyl blue 82-143 amyloid beta precursor protein Rattus norvegicus 35-41 9742949-5 1998 IGFBP-2 secreting clones displayed reduced conversion of thiazolyl blue when compared to negative clones or non-transfected parental 293 cells (P < 0.01). thiazolyl blue 57-71 insulin like growth factor binding protein 2 Homo sapiens 0-7 9809588-1 1998 Alzheimer"s disease amyloid beta protein (Abeta) inhibits cellular reduction of the dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). thiazolyl blue 88-148 amyloid beta precursor protein Rattus norvegicus 42-47 9675425-9 1998 We found that cellular reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) could be inhibited 30-40% by A beta in undifferentiated cells. thiazolyl blue 36-97 amyloid beta precursor protein Rattus norvegicus 133-139 9375659-2 1997 An early indicator of A beta toxicity is the inhibition of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction to MTT formazan, a widely used assay for measuring cell viability. thiazolyl blue 68-128 amyloid beta precursor protein Homo sapiens 22-28 9815713-8 1997 Growth of NPC cells treated with Ad5CMV-p53 was observed to be significantly inhibited when determined by either the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or clonogenic assay. thiazolyl blue 117-178 tumor protein p53 Homo sapiens 40-43 9789077-4 1998 In the present study we show that both Abeta and submicromolar concentrations of free cholesterol alter the trafficking of a population of intracellular vesicles that are involved in the transport of the reduced form of the tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT formazan), the formation of which is a widely used cell viability assay. thiazolyl blue 240-301 amyloid beta precursor protein Homo sapiens 39-44 9620466-2 1998 A novel procedure for the detection of b5R activity in human hemolysates was developed, in which b5R monoclonal antibodies dot-blotted on nitrocellulose membrane was used to capture and enrich b5R from hemolysates, and the captured b5R activity was subsequently visualized with the substrate 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide. thiazolyl blue 292-351 cytochrome b5 reductase 3 Homo sapiens 39-42 9620466-2 1998 A novel procedure for the detection of b5R activity in human hemolysates was developed, in which b5R monoclonal antibodies dot-blotted on nitrocellulose membrane was used to capture and enrich b5R from hemolysates, and the captured b5R activity was subsequently visualized with the substrate 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide. thiazolyl blue 292-351 cytochrome b5 reductase 3 Homo sapiens 97-100 9620466-2 1998 A novel procedure for the detection of b5R activity in human hemolysates was developed, in which b5R monoclonal antibodies dot-blotted on nitrocellulose membrane was used to capture and enrich b5R from hemolysates, and the captured b5R activity was subsequently visualized with the substrate 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide. thiazolyl blue 292-351 cytochrome b5 reductase 3 Homo sapiens 97-100 9620466-2 1998 A novel procedure for the detection of b5R activity in human hemolysates was developed, in which b5R monoclonal antibodies dot-blotted on nitrocellulose membrane was used to capture and enrich b5R from hemolysates, and the captured b5R activity was subsequently visualized with the substrate 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide. thiazolyl blue 292-351 cytochrome b5 reductase 3 Homo sapiens 97-100 9851639-3 1998 The cytotoxic activity of the conjugate, which was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test in the growth inhibition of the neuroblastoma cell line with NCAM expression, was maintained at 65.8% to 94.5% compared with the same concentration of MMC solution. thiazolyl blue 67-127 neural cell adhesion molecule 1 Homo sapiens 200-204 9359461-3 1997 Abeta (1 nM-10 microM) did not cause cell death, but potently inhibited the cellular redox activity as determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay. thiazolyl blue 117-177 amyloid beta precursor protein Rattus norvegicus 0-5 9216088-3 1997 The time-dependent self-assembly of monomeric A beta into fibers was simultaneously monitored by electron microscopy, circular dichroism spectroscopy, analytical ultracentrifugation, and A beta-mediated cellular toxicity using the reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to measure cell viability. thiazolyl blue 244-304 amyloid beta precursor protein Homo sapiens 46-52 9109514-2 1997 In the present study, we found that A beta potently inhibits cellular redox activity of cultured astrocytes, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide reduction assay. thiazolyl blue 130-190 amyloid beta precursor protein Homo sapiens 36-42 9010260-5 1997 Stimulation of MC with IL-1beta and TNF-alpha caused remarkable toxicity to these cells, measured by the MTT test (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide cleavage, specific cytotoxicity 41.5 +/- 20.3%), and much less prominent MC lysis (3H-thymidine release, specific cytolysis 11.5 +/- 5.3%). thiazolyl blue 115-175 interleukin 1 beta Rattus norvegicus 23-31 9010260-5 1997 Stimulation of MC with IL-1beta and TNF-alpha caused remarkable toxicity to these cells, measured by the MTT test (3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide cleavage, specific cytotoxicity 41.5 +/- 20.3%), and much less prominent MC lysis (3H-thymidine release, specific cytolysis 11.5 +/- 5.3%). thiazolyl blue 115-175 tumor necrosis factor Rattus norvegicus 36-45